LOCUS       DASRYE010000001         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_159117,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000001 DASRYE010000000
VERSION     DASRYE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159117"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFF98_00005"
     CDS             <1..254
                     /locus_tag="VFF98_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62035.1"
                     /translation="WVYRQNWQRETDTAAPAGKGERRLSVTIMATAGDRARVSLTEMG
                     AAMPLPASHPPVASAPAQIIALRALPLVILRPIRIGSSA"
     gene            complement(306..614)
                     /locus_tag="VFF98_00010"
     CDS             complement(306..614)
                     /locus_tag="VFF98_00010"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZU62036.1"
                     /translation="MAQDLPHSGPDPVLASYRASIDNIDAALIHMLAERFRITKAVGE
                     HKATHGLPASDPGREDRQIERLRHLAEDAQLDPDFGEKFLRFIISEVIRHHEQAKSEA
                     "
     gene            736..1758
                     /locus_tag="VFF98_00015"
     CDS             736..1758
                     /locus_tag="VFF98_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZU62037.1"
                     /translation="MSANTLPTTGPESAPPPALDSLLALTAPGMACVNAVIRERMASD
                     IPLIPELADHLISSGGKRMRPMLTVASAMLVGFAGDAQYKLAAAVEFIHTATLLHDDV
                     VDGSDQRRGKAAANIVFGNPATVLVGDFLFSRSFELMVEARSLDVLQILANASAVIAE
                     GEVDQLTAQRVVETTEAHYLSIISAKTAALFAAACRIAAVAAGRGPAEEAALDAYGRY
                     LGVAFQLADDAIDYASQSAEMGKDQGDDFRDGKMTLPVILAYADGDAEERRFWQDAIA
                     GFANGDAELARAVAIINRHGAVERTLARARDYAEKAIAALAVFAPGPMRAAMEEAALF
                     AVSRRY"
     gene            1755..>3856
                     /gene="hrpB"
                     /locus_tag="VFF98_00020"
     CDS             1755..>3856
                     /gene="hrpB"
                     /locus_tag="VFF98_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HZU62038.1"
                     /translation="MSRAGEAPLPIAAVLPDLLAALRQGSSAVLIAPPGAGKTTAVAP
                     ALLAEPWCKGRILLLSPRRVAARAAAEHMAEMLGEEAGQTVGYLTRLDSKTSGKTRIV
                     VMTEAIFVATILADPELTGVSAVLFDEAHERHLDSDLGLAMAVETASVLREDLRLLPM
                     SATIDGARFAALLGGAPVIESAGRAYPLAIRWRGGAAEKRVEEQMASAILAAWREEEG
                     DILAFLPGVAEITRTAERLAERLPDTPILPLHGQCEPAAQRAAIRRDGTGRRRIVLAT
                     SIAETSLTLDGVRVVVDSGLARRPEFDKAAGVTRLVTTRASQAAAAQRAGRAARQGPG
                     VAWRLWEEAAHAGRPPFEAPEILTSDLAPLALTLAQWGAGDVAAMAWIDPPPPAAMAA
                     ARAKLASLGALDGAGRITDHGRAMASLPMGPNEAHMLLYAAAHGAQEEAARLALLLQE
                     RDLGGRGDDLLARLERWARERGGKAESARQMARGWAKRARALVAAGRGDGVPTGILLA
                     EAFPDNLAKRRDASGEEWLTAGGRGLVLDPASPLARAEYLAVGAAQGSARGARITSGI
                     ALTEAEVLKHLAHRIERRSALRWVADEARVEALLEQRLGAITFARGPDPAPDRAAIVA
                     FLCARIRAEGLGLLPLGKASQALLKRARYAGLAAFAEDALLADLEGWAGPHLHRRLDQ
                     VQAGALHQALADRLGWEG"
BASE COUNT          524 a         1303 c         1434 g          595 t
ORIGIN      
        1 cctgggtcta tcgccagaac tggcagcgcg agaccgacac cgccgcgccc gccggcaagg
       61 gcgagcggcg cctgtccgtc accatcatgg ccaccgcggg cgatcgcgca cgcgtcagcc
      121 tgaccgaaat gggcgcggcc atgccactgc ccgccagcca cccgccggtg gccagcgcgc
      181 cggcccagat catcgccctc agggccttgc cactcgtcat cttgcgcccc atccgcattg
      241 gctcatccgc ctaggcttct ccgcaaggca ccctagggcc ctttgcggag ggcgcaaggg
      301 gctgttcagg cttcgctttt ggcctgctcg tggtggcgga tcacttcgct gatgatgaag
      361 cgcaggaatt tctcgccgaa atcggggtcg agctgggcat cctcggccag gtggcgcagc
      421 cgctcgatct gccggtcctc gcggccgggg tcggaagcgg gcaggccatg ggtggccttg
      481 tgctcgccca ccgccttggt aatgcgaaag cgctcggcca gcatgtggat gagggcagca
      541 tcgatattgt cgatactggc gcgatagctg gccagcaccg ggtcgggccc ggaatggggg
      601 agatcttgtg ccatgccaga gcgctagcac gcgggcgccg cggtttccag accgcagctc
      661 tttagccggg cggtttggcc gttatcgacg ccttaggact tgccttcgcg cgcgggccgg
      721 cgcaatgcgc gctcgatgag cgccaacacc cttcccacca ccggacccga atccgccccg
      781 ccccccgcgc tggattccct gctggcgctg acggcgccgg gcatggcctg tgtcaacgcg
      841 gtgatccgcg agcgcatggc cagcgacatc ccgctgatcc ccgaactggc ggaccatctg
      901 atctccagcg gcggcaagcg catgcgcccg atgctgacgg tggccagcgc gatgctggtc
      961 ggctttgcgg gcgatgcgca atacaagctc gccgccgcgg tggaattcat ccacaccgcc
     1021 accctgctgc atgatgatgt ggtggatgga tcggaccagc ggcgcggcaa ggcggcggcc
     1081 aatatcgtct ttggcaatcc ggccacggtg ctggtgggcg atttcctgtt ctcgcgcagc
     1141 ttcgagctga tggtggaagc gcgcagcctt gatgtgctgc agatcctggc caacgcctcg
     1201 gcggtgatcg ccgagggcga ggtggaccag ctgaccgccc agcgcgtggt ggaaaccaca
     1261 gaggcgcatt acctctcgat catctcggcg aaaaccgccg cgctgtttgc cgccgcctgc
     1321 cgcattgccg cggtggccgc cgggcgcggg ccggccgagg aagccgcgct cgatgcctat
     1381 ggccgttatc tgggcgtggc cttccagctg gccgacgatg ccatcgacta tgcctcgcaa
     1441 agcgccgaaa tgggcaagga ccagggcgat gatttccgcg atggcaagat gaccctgccg
     1501 gtgatcctgg cctatgccga tggcgatgcc gaggagcgcc gcttctggca ggatgccatt
     1561 gccggctttg ccaatggcga tgccgaactg gcccgggcgg tggcgatcat caaccgccat
     1621 ggcgcggtgg agcgcacgct ggcgcgcgcg cgtgactatg ccgagaaggc gatcgcggcg
     1681 ctggcggtgt ttgcgcccgg cccgatgcgc gcggcgatgg aagaggcggc gctgttcgcg
     1741 gtgtcgcgcc gctattgagc cgggctgggg aggcgccgct gccgattgcg gcggtgctgc
     1801 ccgatctgct ggcggcgctg cggcagggct catcagcggt gctgatcgcc ccgcccggcg
     1861 ccggcaagac caccgccgtt gcccccgccc tgctggccga accctggtgt aaggggcgca
     1921 tcctgctgct gtcgccccgc cgcgtggccg cccgtgccgc ggccgagcac atggccgaga
     1981 tgctgggcga ggaagccggg cagacggtgg gctatcttac ccggctcgat tcgaagacca
     2041 gcggcaaaac ccgcatcgtg gtgatgaccg aggcgatctt cgttgccacc attctcgctg
     2101 atcctgagct gacgggcgtt tcggcggtgt tgttcgacga ggcgcatgag cggcatctcg
     2161 attcggatct ggggctggcg atggcggtgg aaacggccag cgtgctgcgc gaggatctgc
     2221 gcctgctgcc gatgtcggcc actatcgatg gcgcgcgttt tgccgcgctt ttgggcggcg
     2281 ccccggtgat cgagagcgcg gggcgcgcct atccgctggc gatccgctgg cggggcgggg
     2341 ctgcggaaaa gcgcgtggaa gagcagatgg ccagcgccat ccttgccgcc tggcgcgagg
     2401 aggaggggga tatcctcgcc ttcctgccgg gcgttgccga aatcacccgc actgccgagc
     2461 ggctggccga gcgcttgccc gacacgccga tcctgccgct gcatggccag tgcgagccgg
     2521 ccgcccagcg cgcggccatc cgccgcgatg gcaccgggcg gcggcgcatc gtgctggcca
     2581 ccagcattgc cgaaacctcg ctgacgctgg atggcgtgcg ggtggtggtc gattcggggc
     2641 tggcccgccg gccggagttc gacaaggccg ccggggttac ccggctggtc accacgcgcg
     2701 ccagccaggc ggccgccgcc cagcgcgccg gccgcgccgc gcggcagggg ccgggcgtgg
     2761 cctggcgcct gtgggaagag gccgcccatg ccgggcgccc gcccttcgag gcgccggaaa
     2821 tcctcaccag cgatctggcg ccgctggcgc tcacgctggc gcagtggggt gcgggcgatg
     2881 tggccgccat ggcctggatc gatccgccgc cgccggccgc catggccgcc gcccgggcca
     2941 agctcgccag ccttggcgcg ctggatgggg ccgggcgcat caccgatcat ggccgcgcca
     3001 tggccagcct gccgatgggg ccgaatgagg cgcatatgct gctttatgcc gccgctcatg
     3061 gggcgcagga ggaagccgcg cgcctggccc tgctgctgca ggagcgcgat cttggcgggc
     3121 gcggcgatga tttgctggcc cggctggaac gctgggcgcg cgagcgtggc ggcaaggcgg
     3181 aaagcgcccg ccagatggcg cgcggctggg cgaagcgcgc gcgggcgctg gtggcggcgg
     3241 gccggggcga tggcgtgccc accggcatcc tgctggccga ggccttcccc gacaatctgg
     3301 ccaagcgccg cgatgcctcg ggcgaggaat ggctgacggc agggggcagg ggcctcgtgc
     3361 tcgatccggc cagcccgctg gcccgggcgg aatatctggc ggtgggcgcg gcgcaaggca
     3421 gcgccagggg cgcgcgcatc accagcggca tcgcgctgac cgaagccgaa gtgctgaagc
     3481 atctggccca ccggatagag cgccgcagcg ccctgcgctg ggtggccgat gaggcgcggg
     3541 tggaagctct gctggagcag cggctgggcg ccatcacctt tgcccgcggg ccggatccgg
     3601 cgcccgatcg cgccgctatc gtcgctttcc tgtgcgcgcg gatccgggcc gaggggctgg
     3661 gcctgctgcc gctgggcaag gccagccagg cgctgttgaa gcgggcgcgt tatgccgggt
     3721 tggccgcctt tgccgaggac gcgctgctgg ccgatctgga aggctgggcc gggccgcatc
     3781 tgcaccgccg gctcgatcag gtgcaggccg gcgccttgca tcaggcgctg gcggaccggc
     3841 tgggctggga gggcaa
//
LOCUS       DASRYE010000002         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_95, whole
            genome shotgun sequence.
ACCESSION   DASRYE010000002 DASRYE010000000
VERSION     DASRYE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1144
                     /gene="crtY"
                     /locus_tag="VFF98_00025"
     CDS             125..1144
                     /gene="crtY"
                     /locus_tag="VFF98_00025"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HZU62039.1"
                     /translation="MPGRWKQYEVRFPRLGRIIHTPYRSLSGRRLDAALRESLPADSL
                     LANACVTRLDATSLTLENGRTLTAGVVIDARGASAFPGLTGGWQKFLGQLVRLERPHG
                     ITTPMLMDGRVPQRDGFRFQYILPFSADTLFIEETYYTTDPALDAATLRRRIADYLQL
                     SGFAVAELLEEEQGLLPVVARGRLEALRQGLGTDRPEAAPAIGLRAGLFHPLTGYSLP
                     AAAETALLVADLPELTPARLHRALRHHTRAHWQRGAYCRMLARMLFGATRGGRRFKVF
                     ERFYRLDEGLIERFYAGRLTGRDKLRIVSGKAPVPVSRAIASLLGGGLPLARLDAPQA
                     AKEGE"
     gene            1145..2626
                     /locus_tag="VFF98_00030"
     CDS             1145..2626
                     /locus_tag="VFF98_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HZU62040.1"
                     /translation="MKKACVIGAGFGGLALAIRLQSAGIATTIVEARDRPGGRAYVWQ
                     REGFTFDAGPTVITDPAALEELWALSGQRLADDVELLPVMPFYRLHWADGTTFDYSND
                     EVQLRAQIARMAPGDLAGYEDFARYAAEVYEEGYVKLGHVPFLDFASMVKAAPALLRH
                     QAWRSVYAKVASCVKSEKLRQALSFHTLLVGGNPMATSSLYALIHKLEKDGGVWWARG
                     GTNRLVAGMVALFERLGGTLRLDDKVRQIHTIGTRATEVETASGWRERFDAVASNADI
                     MHTYRDLLVGTPRGAAQAQKLARKSWSPSLFVVHFALEGSWPGIPHHMILFGPRYRGL
                     LADIFDHGVLPRDMSIYLHHPSVTDPSMAPEGKSTFYALVPVAHMGKLTIDWAEVGPL
                     LARRVLDEVGRRLIPDIHDRVITSFHYAPADFAQDLGAYMGSAFSLEPLLTQSAWFRA
                     HNRDDVIENLYLVGAGTHPGAGIPGVVGSAKATAGLMIEDLRL"
     gene            2651..3232
                     /locus_tag="VFF98_00035"
     CDS             2651..3232
                     /locus_tag="VFF98_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZU62041.1"
                     /translation="MKRIAVYCGSATPADPRYIELARQVGETLARRGIGLVYGGGRLG
                     LMGAVADAALAAGGEVIGVIPVALSRAEVAHHGCTRLIEVETMHERKAAFVEFADGFL
                     TLPGGVGTMDELWEAVSWAQLGYHAKPVGLLNAFGFYDTLLAFARHMGEVGFVRPAHQ
                     GILLAESELDLLLARMAAHEPIAPIIRMDPSTL"
     gene            3229..4173
                     /locus_tag="VFF98_00040"
     CDS             3229..4173
                     /locus_tag="VFF98_00040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZU62042.1"
                     /translation="MILPRAQLVAAARASIAAGSKSFAAASALFGRTTRERVWLLYAW
                     ARAADDIADGQVLGGALAAPADEAANLARIRRLTALALAGEPTGDAAFDALGVVAAEC
                     GISAVHTGDVITGFALDAAGWRPQTELELLGYCYHVAGAVGVLMALVMGVPPEDHDTL
                     DRACDLGIAFQLGNILRDIAEDHAGGRCYLPAQWLAEAGIAEADLMAPQHRPALAGMA
                     ARLGARAQGYEASARVGAARLPLRCRWAVLSAAGIYGAIARQVVRRGPAAWNSRVTTS
                     NAAKLGFVVLGLAQALLPARSCPPVPYARAQLARAALS"
     gene            complement(4166..>5079)
                     /locus_tag="VFF98_00045"
     CDS             complement(4166..>5079)
                     /locus_tag="VFF98_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62043.1"
                     /translation="GLACSGLMVLPATIIRDRLEGDAMARMQSTVAMVFMIVPMLAPS
                     VGQAVLAVAGWRWIFGMMAVLGLAVAGWAFVRLPETLRPEFRQSIVPLQILRTMIHVV
                     TLREAFGYVFAMAGVQGAMFAYINSSQQLVAEHFGAGARFPLIFAGMAAMMASANFVN
                     SRIVERFGARRVSHTALLIAIVTSLTQLALASRPGETLWQFVPVMTVNMCMLGFLGAN
                     FSSIALQPFARNAGAAASGQSFVRMVLAAGLGSLVGQAYDGTARPLAMTLVGTAVVGL
                     ALVLVSERGRLFRRVLPPGSRREMGFS"
BASE COUNT          773 a         1766 c         1758 g          782 t
ORIGIN      
        1 ccgcccgaaa acgagacggc tgccggccat gctgccccgc cggcgccgac attgccgata
       61 ttgccgatat tgccgacatt gccggccctg gcgctgctga tggcggcgcc tttcggcccg
      121 cccgatgccg gggcgctgga agcaatatga agtgcgcttt ccgcgcctgg gtcggatcat
      181 tcacacgccc tatcgcagcc tttccggccg ccggctggat gcggccctgc gcgaaagcct
      241 gccggccgac agcctgctgg ccaacgcctg cgtcacccgc ctcgatgcca ccagcctgac
      301 gctggaaaac gggcgcaccc ttaccgccgg cgtggtgatc gatgcgcgcg gggccagcgc
      361 ctttccgggg ctgacgggcg gctggcagaa attcctgggc cagctagtgc ggctggaacg
      421 gccccatggc atcaccacgc cgatgctgat ggatggccgc gtgccccagc gcgatggctt
      481 ccgcttccag tatatcctgc cgttcagcgc cgacacgctg ttcatcgagg aaacctatta
      541 caccaccgat cccgcgctgg acgcggccac gctgcgccgc cgcatcgccg attatctgca
      601 gctgagcggc tttgccgtgg ccgagctgct ggaagaggaa caggggctgc tgccggttgt
      661 cgccagaggg cggctggagg ccctgcgcca ggggctgggc accgaccggc ccgaggccgc
      721 cccggcgatc ggcctgcgcg cggggctgtt ccatccgctc accggctatt cgctgccggc
      781 cgcggccgaa accgcgctgc ttgtcgctga cctgcccgag ctgacccctg cccgcctgca
      841 ccgcgccctg cgccaccaca cccgcgccca ctggcagcgc ggcgcctatt gccggatgct
      901 ggcgcgcatg ctgttcgggg ccacgcgcgg cggccgccgc ttcaaggtct tcgagcgctt
      961 ctaccggctg gacgaggggc tgatcgagcg cttctacgcc gggcggctga cagggcgcga
     1021 caaactgcgt attgtgtcgg gcaaggcacc ggtgccggtc agccgggcga tcgccagcct
     1081 gctgggcggc ggcctgccgc tggcccggct ggatgcgccg caggccgcca aggaaggtga
     1141 ataggtgaag aaagcctgtg tgatcggggc cggatttggc gggctggcgc tggccatccg
     1201 cctgcaatcg gccggcatcg ccaccaccat cgtcgaagcg cgggaccggc cgggcgggcg
     1261 cgcctatgtc tggcagcgcg agggcttcac cttcgatgcc ggccccaccg tcatcaccga
     1321 tccggcagcc ctggaggaat tgtgggcgct ttcgggccag cgcctggccg atgatgtgga
     1381 gctgctgccg gtgatgccgt tctaccggct gcactgggcc gatggcacca cgttcgacta
     1441 ttcgaacgac gaggtgcagc tgcgcgccca gatcgcccgc atggcccccg gagacctcgc
     1501 cggctacgag gatttcgccc gctatgccgc cgaggtctac gaggaaggct atgtgaagct
     1561 gggccatgtg cccttcctcg attttgccag catggtgaag gcggccccgg cgctgctgcg
     1621 ccatcaggcg tggcgctcgg tctatgccaa ggtggcctcc tgcgtgaaat cggagaagct
     1681 gcgccaggcg ctgtcgttcc acacgctgct ggtgggcggc aatcccatgg ccaccagcag
     1741 cctctatgcg ctgatccaca agctggaaaa ggatggcggg gtgtggtggg ccaggggcgg
     1801 caccaaccgg ctggtggccg gcatggtggc cctgttcgag cggctgggcg gcaccctccg
     1861 gctggacgac aaggtgcgcc agatccacac catcggcacc cgcgccaccg aggtggaaac
     1921 cgccagcggc tggcgcgagc gtttcgatgc agtggccagc aacgccgata tcatgcacac
     1981 ctaccgcgac cttctggtcg gcaccccgcg cggggccgcc caggcgcaga agctggcacg
     2041 caagagctgg tcgcccagcc tgttcgtcgt ccatttcgcg ctggaaggca gctggccggg
     2101 cattccccat cacatgatcc tgttcggccc gcgctatcgc ggcctgctgg ccgatatttt
     2161 cgaccatggc gtgctcccgc gcgacatgtc gatctatctg caccaccctt ccgtcaccga
     2221 tccttcgatg gcgcccgagg gcaagagcac tttctacgcg ctggtgccgg tggcccatat
     2281 gggcaagctg acgatcgact gggccgaggt gggcccgctg ctggcccggc gcgtgctcga
     2341 tgaagtgggc cgccgcctca tccccgatat ccacgaccgg gtgatcacca gcttccatta
     2401 tgcccctgcc gatttcgcgc aggatctcgg cgcctatatg ggcagcgcct tcagcctgga
     2461 gccgctgctg acccagagcg cctggtttcg cgcccacaac cgcgacgatg tgatcgaaaa
     2521 cctctatctg gtgggcgcgg gcacccatcc gggggcgggc attcccggcg ttgtcggcag
     2581 cgccaaggcc accgccgggc tgatgatcga ggacttgcgc ctgtaacgcc aagccggcac
     2641 taaggcggtc atgaaacgca ttgcagtcta ttgtggctcg gccacgcccg cagatccccg
     2701 ctatatcgaa ctggcccgcc aagtgggcga aacgctggcc cggcgcggca ttggcctggt
     2761 ctatggcggc gggcggctgg ggctgatggg ggcggtggcc gatgccgcgc tggcggccgg
     2821 cggcgaggtg atcggggtga tcccggtggc gctttcgcgc gcggaagtgg cgcatcatgg
     2881 ctgcacccgc ctgatcgagg tggaaaccat gcatgagcgc aaggccgcct tcgtggaatt
     2941 tgccgatggc ttcctcaccc tgcccggcgg cgtgggcacg atggacgagc tgtgggaagc
     3001 ggtgagctgg gcgcagctgg gctatcatgc caagccggtg ggcctgctca acgccttcgg
     3061 cttttacgac acgctgctgg cctttgcccg ccacatgggc gaggtgggct ttgtgcggcc
     3121 ggcccatcag ggcatcctgc tggccgaaag cgagctggac ctgctgctgg cccgcatggc
     3181 cgcgcatgag ccgatcgcgc cgatcatccg gatggatccg agcacgttgt gatcctgccg
     3241 cgcgcgcagc tggtggcggc cgcgcgggcc agcatcgcgg ccggttccaa aagctttgcc
     3301 gccgccagcg ccctgtttgg ccggaccacg cgtgagcggg tgtggctgct ctatgcctgg
     3361 gcccgcgcgg cagacgacat tgccgacggg caggtgctgg gcggcgcgct ggcagcgccg
     3421 gcggatgaag cggccaacct tgcccgcatc cgccggttga ccgctctcgc gctggcgggc
     3481 gagccgaccg gcgatgcggc cttcgacgcg ctgggcgtgg tggcggccga atgcggcatc
     3541 agcgccgtgc acaccggcga tgtgatcacc ggctttgcgc tggatgccgc cggctggcgc
     3601 ccgcagacgg agctggaact gctgggctat tgctaccatg tggcgggcgc cgtgggagtg
     3661 ctgatggcgc tggtgatggg cgtgccgccg gaagaccacg acacgctcga tcgcgcctgt
     3721 gacctcggca ttgccttcca gctgggcaac atcctgcgcg atatcgccga ggaccatgcc
     3781 ggcgggcgct gctatctgcc ggcgcagtgg ctggcagagg ccggcattgc cgaggccgac
     3841 ctcatggccc cgcagcaccg cccggcgctg gccggcatgg cggcaaggct gggcgcgcgg
     3901 gcgcagggct atgaggcttc ggcacgggta ggggcggcgc ggctgccgtt gcgctgccgc
     3961 tgggcggtgc tgtcggccgc cggcatctat ggggcgatcg cccgccaggt ggtgcgccgg
     4021 ggccctgccg catggaacag ccgcgttacc acctcaaatg cggccaagct gggctttgtg
     4081 gtgctggggc tggcgcaggc gctgctgccg gcgcgcagct gcccgcccgt gccttacgcg
     4141 cgcgcgcagc tggcccgcgc cgccctcagc tgaagcccat ctcgcggcgg ctgccgggcg
     4201 gcagcacccg gcggaacagg cgcccgcgct cgctgaccag caccagcgcc agccccacca
     4261 cggcggtgcc caccagcgtc atcgccagcg ggcgggcggt gccgtcatag gcctggccaa
     4321 ccagcgagcc caggcccgcg gccagcacca tgcgcacgaa agactggccc gaggcggcag
     4381 cgcccgcatt gcgggcaaag ggctgcagcg cgatggacga gaaattggcg cccagaaagc
     4441 ccagcatgca catgttcacc gtcatcaccg gcacgaattg ccacagcgtc tcgcccggcc
     4501 ggctggccag cgccagctgc gtcagcgagg tgacgatggc aatcagcagc gccgtatgcg
     4561 acacgcgccg cgcgccaaac cgctcgacaa tgcgcgaatt gacgaaattg gcgctggcca
     4621 tcatcgccgc catgccggca aagatcagcg ggaagcgggc gccggcgcca aaatgctcgg
     4681 ccaccagctg ctgcgaggaa ttgatatagg caaacatcgc cccctgcacg ccggccatgg
     4741 caaacacata gccaaacgcc tcgcgcaggg tgacgacatg gatcatcgtg cgcaggatct
     4801 gcagcggcac gatcgactgg cggaattcgg gccgcagcgt ttcgggcagg cgcacgaagg
     4861 cccagcccgc caccgccagc cccagcaccg ccatcatgcc gaagatccac cgccagccgg
     4921 ccaccgccag caccgcctgg ccaacgctgg gcgccagcat cggcacgatc atgaacacca
     4981 tcgccacggt ggattgcatg cgcgccatgg catcgccttc cagcctgtcg cggatgatgg
     5041 tggcaggcag caccatcagg cccgaacagg ccagcccca
//
LOCUS       DASRYE010000003         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_141523,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000003 DASRYE010000000
VERSION     DASRYE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141523"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFF98_00050"
     CDS             <1..1292
                     /locus_tag="VFF98_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU62044.1"
                     /translation="RHLFFAEREAGRIPGLPAGTAARPLRKAAVIGAGTMGGGIAMCF
                     AGAGIPVALIDTTEEALARGLDRVKGNYATSLKRGSISQAQMDERLALISPATARAAA
                     ADADIVIEAVFEDMAVKQEIFADLERQVRPGTVLASNTSALDIDAIAGVLARPEDFVG
                     MHFFSPANVMKLLEVVKGAKTGADAIMTAMAVGRAIGKVPVLSGNCDGFIGNRMVAKR
                     SAQAERLLQQGALPQQVDAALTHLGFPMGPLTTNDMSGLDIGYAIRKRRGTPFPIADA
                     IVESGRLGQKTGAGYYRYEPGDRTPRPNPETEALILATSQQLGITRREIAEAEMVERL
                     LFPLINEGARILEEGIALRPSDVDLVWVNGYGWPRHLGGPMFYADEQGLAYIVGRLRQ
                     FAEETPEDPSLYPARLLIELSVRGESFAHWQKAQSPA"
     gene            1349..2185
                     /locus_tag="VFF98_00055"
     CDS             1349..2185
                     /locus_tag="VFF98_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU62045.1"
                     /translation="MSEENEFLRPIDYSEFDFFTVEIGADGVAVVTMNDPDRLNAVGP
                     HNHWQIEDIWLKLARDERIKVIVLTGAGKAFSAGGDIKLMAQRAQTEYGLKYALRVPM
                     NTMRIWDQILLTPQPIIAAVNGDAIGLGTSLFAFCDMSIVAEDARLGDTHVRVGLVTG
                     DGGSVMWPLLVGPQRAKEYLMRGKLLDGKKAEEIGLVNYAFPREAVLDEALKIAREIA
                     GQPVWAVRWSKAAVNKQLKAQVNLIGELSIAYEAMTMLTHDYKAATTAFANKEKPVFK
                     GY"
     gene            2191..>2530
                     /locus_tag="VFF98_00060"
     CDS             2191..>2530
                     /locus_tag="VFF98_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU62046.1"
                     /translation="MAAYRDTARIATVTAIGAGVIGAGWVAAFLGSGRAVRLFDPAPD
                     AAARAAAHVAAAWPQMAALGLAPDHADWRCRLTTHDSLAEATAGTDFVQESTPERLDL
                     KQALLAELDRL"
BASE COUNT          428 a          825 c          874 g          403 t
ORIGIN      
        1 tgcgccacct gttctttgcc gagcgcgagg ccggccgcat ccccggcctg cccgccggca
       61 ccgcggcccg ccccttgcgc aaggccgcgg tgatcggcgc cggcacgatg ggcggcggca
      121 ttgccatgtg ctttgccggc gccggcatac ccgtggccct gatcgacacc accgaggaag
      181 cgctggcccg cggcctcgac agggtgaagg gcaattacgc cacctcgctc aagcgcggct
      241 cgatcagcca ggcgcagatg gacgagcggc tggcgctgat ctcgcccgcc accgcccgcg
      301 ccgctgccgc cgatgccgat atcgtgatcg aggcggtgtt cgaggacatg gccgtgaagc
      361 aggagatctt cgccgatctg gaacggcagg tgcggcccgg cacggtgctg gcctccaaca
      421 cctcggcgct cgatatcgat gcgatcgccg gcgtgctggc ccggccggaa gatttcgtcg
      481 gcatgcattt cttctcgccg gccaatgtga tgaagctgct ggaagtggtg aagggggcca
      541 aaaccggcgc cgatgccatc atgacggcga tggccgtggg ccgggcgatc ggcaaggtgc
      601 cggtgctttc gggcaattgc gatggtttca tcggcaaccg catggtggcc aagcgctctg
      661 cccaggccga gcggctgctg caacagggcg ccctgccgca gcaggtggat gccgccctca
      721 cccatctggg cttcccgatg ggcccgctca ccaccaacga catgtcgggc ctggatatcg
      781 gctatgccat ccgcaagcgg cgcggcacgc cctttcccat tgccgatgcc atcgtcgaga
      841 gcgggcggct ggggcagaag acgggggccg gctattaccg ctatgaaccg ggcgatcgca
      901 ccccccgccc caacccggag accgaggcgc tgatcctcgc cacatcgcag cagctgggca
      961 tcacccggcg cgagatcgcc gaggccgaga tggtggagcg cctgctgttc ccgctgatca
     1021 acgagggcgc gcgcattctg gaagagggca ttgccctgcg cccgtcggat gtcgatctgg
     1081 tgtgggtgaa cggctatggc tggccgcgcc atctgggcgg gccgatgttc tatgccgatg
     1141 agcaggggct ggcctatatc gtcggccggc tgcgccagtt tgccgaggag acgccggagg
     1201 atccctcgct gtatccggcg cgattgctga tagaactgtc tgtccgcggc gaaagcttcg
     1261 cccattggca aaaggcccag agcccggcct gacaggcccc aaccgacttg ccggccaggc
     1321 aagccccaac cctttccagg agagccccat gagcgaagaa aatgaattcc tccgcccgat
     1381 cgactacagc gagttcgatt tcttcacggt cgagatcggc gcggatggcg tggctgttgt
     1441 caccatgaac gatcccgacc ggctgaacgc ggtgggcccg cacaaccact ggcagatcga
     1501 ggacatctgg ctgaagctgg cgcgcgatga acgcatcaag gtgatcgtgc tgacgggcgc
     1561 cggcaaggcc ttctcggccg gtggcgacat caagctgatg gcccagcgcg cgcagaccga
     1621 atatggcctg aaatatgccc tgcgcgtgcc gatgaacacg atgcgcatct gggatcagat
     1681 cctgctgacg ccgcagccga tcatcgcggc ggtaaacggc gatgccatcg gccttggcac
     1741 ctcgctgttc gccttttgcg acatgagcat cgtggccgag gatgcccggc ttggcgatac
     1801 ccatgtgcgc gtggggctgg tcaccggcga tggcggatcg gtgatgtggc cgctgctggt
     1861 ggggccgcag cgggccaagg aatatctgat gcgcggcaag ctgctggatg gcaaaaaggc
     1921 cgaggagatt ggcctggtca actatgcctt cccgcgcgag gcggtgctgg acgaggcgct
     1981 gaagatcgcc cgcgagatcg ccggccagcc ggtctgggcg gtgcgctggt cgaaggcggc
     2041 ggtgaacaag cagctgaagg cgcaggtcaa cctgatcggc gaactgtcga tcgcctatga
     2101 ggcgatgacc atgctgacgc atgactacaa ggccgccacc acggccttcg ccaataagga
     2161 aaagcccgtt ttcaagggct attgaacagg atggcggcct atcgcgacac ggcgcgcatc
     2221 gccaccgtca ccgccattgg cgcaggggtg atcggcgctg gctgggtggc cgctttcctg
     2281 ggcagcgggc gggcggtgcg gctgttcgat ccggcgcccg atgccgccgc gcgcgccgcg
     2341 gcccatgtgg ccgcggcctg gccgcagatg gccgcgctgg gcctggcgcc cgaccatgcc
     2401 gactggcgct gccgcctgac cacgcacgac agcctggccg aagcgacagc aggcaccgat
     2461 ttcgtgcagg aaagcacgcc cgagcggctc gatctcaaac aggcgctgct ggccgagctg
     2521 gaccggctgg
//
LOCUS       DASRYE010000004         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_176907,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000004 DASRYE010000000
VERSION     DASRYE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176907"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VFF98_00065"
     CDS             <1..160
                     /locus_tag="VFF98_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZU62047.1"
                     /translation="GISGAIQHLAGMKDSKTIIAINKDEDAPIFQVADIGLVADLFSA
                     VPELTGKL"
     gene            complement(203..850)
                     /locus_tag="VFF98_00070"
     CDS             complement(203..850)
                     /locus_tag="VFF98_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HZU62048.1"
                     /translation="MSASDRLEEEVALLRAALAPADARRPQAAIDRHMARLLRLIAPR
                     RRHFIRAYGLLDAAEDAEQACAIALWRAILLYDPQRARFTTLLNWQLRGELQALRQRL
                     RRGRGWQAVSLDLLLEQEESALPGDGAAQAAAEAGAAGVMARQACGRLFEAYAGRLRQ
                     NGRAGPLTEARLGEDRQALWRHLFAGAEDGAARTAPEEVALRRTLRGLRQAAQWP"
     gene            complement(956..1291)
                     /locus_tag="VFF98_00075"
     CDS             complement(956..1291)
                     /locus_tag="VFF98_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62049.1"
                     /translation="MTTAPSRSAFSSSLPVVAAVAGWRCRCAGACSGLQAARCLQESA
                     LALFSGQCGKASAHALMPPLAVLVARQKSREGQMECAALARALAAHDHAAVLDACRRM
                     IELESQQAA"
     gene            complement(1361..2551)
                     /locus_tag="VFF98_00080"
     CDS             complement(1361..2551)
                     /locus_tag="VFF98_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HZU62050.1"
                     /translation="MRFPSGMSGTPDPARPLRWLAGGLLVAMGLVFAIGRRQVAAHPL
                     WQWPLAFAEAALVGGLADWFAVTALFRRPLGLPIPHTAIIPTNRDRLADSLARFVERH
                     FLVPAALARRLRGVDFAALAAGEGAGAGWRAPLRQALGEALAALPEAEVAGLLRDLAA
                     GLAARMDLPALVGARLAAAVGAGRHEDGVAAALRWLGGRLEAHEDLLRAVVHARSINL
                     LHWTGLDEALADRLLDGACRVLAEAVVDPAHPLRRRITGELAELARRLQEDAALRARL
                     AALQAQWLGGASGAALMAALWRGLRGGLAGGLPPLPQLLAEPAARWRFNRHVRRLLVG
                     LASRHGGAIASLVSTTIRSWDSTTLTARMEGAIGRDLQYIRLNGTLVGGLLGLALHAL
                     GLLG"
     gene            complement(2658..2957)
                     /locus_tag="VFF98_00085"
     CDS             complement(2658..2957)
                     /locus_tag="VFF98_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HZU62051.1"
                     /translation="MIENQKIRPAMVIGPLGEPLTLESLPPPTTTRWVVRRKAEVVAA
                     VNGGLLTIDEVCERYGLTLEEFASWQRAVDRSGMQGLRVTRIQHYRDLYERQFKY"
     gene            complement(2954..3238)
                     /locus_tag="VFF98_00090"
     CDS             complement(2954..3238)
                     /locus_tag="VFF98_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62052.1"
                     /translation="MVYLLLTLPGYKGRMDKISTQDPGIGASGAISTGRKTAAQQAGH
                     RFIPQDRAWPAARGRGSSAVNPVALGDFKTNPGISLGSAGPLPTGDRGLQ"
     gene            3585..>3975
                     /locus_tag="VFF98_00095"
     CDS             3585..>3975
                     /locus_tag="VFF98_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZU62053.1"
                     /translation="MSGGVDSSVVAALAAASGAEVIGITLQLYDHGEAVKRKGACCAG
                     DDIRDARAVADRLGIAHYVFDHESSFREEVVERFAEEYLAGRTPIPCIRCNMGPKFTD
                     LFAMARDLGADCLATGHYVRRVAGPAGA"
BASE COUNT          607 a         1478 c         1360 g          530 t
ORIGIN      
        1 cggcatttcc ggcgccatcc agcacctcgc cggcatgaag gacagcaaaa ccatcatcgc
       61 catcaacaag gacgaagacg cacccatctt ccaggtcgca gacatcggcc tcgtcgccga
      121 cctcttctca gccgtgccgg agctgaccgg caagctttga ggcttttgcg ccggcgcggg
      181 gggcttcttc gcgccggcgt gcctagggcc attgcgccgc ctggcgcagg ccgcgcaggg
      241 tgcgccgcag ggccacttct tccggcgcgg tgcgggcggc gccgtcctct gcgccggcga
      301 acaggtggcg ccagagggcc tgacggtcct cgcccagccg tgcctcggtg agggggcctg
      361 cccgaccgtt ctggcgcaac cggccggcat aggcttcgaa caggcgccca caggcctgcc
      421 gcgccatcac cccggcagca cctgcttcag cggcggcctg cgcggcgcca tcgcccggca
      481 gggcgctctc ctcctgctcc agcagcagat ccagcgacac cgcctgccag ccccggcccc
      541 gccgcagccg ctggcgcagc gcctgcaatt cgccgcgcaa ttgccagttg agcagggtgg
      601 tgaagcgggc ccgctggggg tcgtagagca aaatggcgcg ccacagggcg atggcgcagg
      661 cctgttcggc atcttcggcg gcatcgagca gcccgtaggc gcggatgaaa tgccgccgac
      721 gcggcgcgat cagccgcagc aggcgggcca tgtggcgatc tatcgcggcc tgcggacggc
      781 gcgcgtctgc cggcgccagc gcggcgcgca gcagggccac ttcctcctca agcctgtcgc
      841 tggccgacat gtccttgtcc tgtcaaagcc gggcgggccg gcagcaggca aggctaggcg
      901 ccggcggctg aagaaagcgg ccacacgccc ccccgggaaa agccccgatg ggcgctcagg
      961 cggcctgctg gctttccagc tcgatcatcc ggcggcaggc atccagcacg gcggcatgat
     1021 catgcgcggc cagcgcccgg gccagcgctg cgcattccat ctgcccctcg cggcttttct
     1081 ggcgcgccac cagcacggcc agcggcggca tcagcgcatg ggccgacgcc ttgccgcatt
     1141 ggccgctgaa cagggccagc gcgctttcct gcaggcagcg ggccgcctgc agccccgaac
     1201 aggccccggc gcaacggcag cgccagcctg ccaccgccgc cacaaccggc agggaagagg
     1261 aaaaggcaga acggctgggc gcggtggtca tgcctgccat tatagcggcc gggcggcctg
     1321 ttccgtcagg cccggcgctt ttgccccggc gcgcccttgc tcagcccagc aggcccagcg
     1381 catgcagcgc cagccccagc aggccaccca ccagcgtgcc gttgaggcgg atatactgca
     1441 gatcgcggcc aatcgcccct tccatccggg cggtgagcgt ggtgctgtcc cagctgcgga
     1501 tggtggtgga caccagcgag gcgattgccc cgccatggcg gctcgccagc cccaccagca
     1561 ggcgccgcac atgccggttg aaacgccagc gggcagcggg ctcggccaac agctggggca
     1621 atgggggcag gccgccggcc agcccgccgc gcaggccccg ccacagcgcc gccatcagcg
     1681 ccgcgccgga agcaccgccc agccattgcg cctgcagcgc ggcaaggcgg gcgcgcaagg
     1741 cggcatcctc ctgcaaccgg cgggccagtt cagccagctc gccggtgatg cggcggcgca
     1801 aggggtgggc cgggtccacc accgcttcgg ccagcacccg gcaggcgcca tccagcagcc
     1861 ggtcggccag ggcctcgtcc agccccgtcc agtgcagcag gttgatcgag cgggcatgga
     1921 ccaccgcgcg cagcagatcc tcatgcgctt ccagccgccc gcccagccag cgcagggccg
     1981 ccgccacgcc atcctcgtgc cggccagcgc ccaccgcggc cgccagccgg gcgcccacca
     2041 gcgcgggcag atccatccgc gcggccagcc ccgcggccag atcgcgcagc aggccggcca
     2101 cttcggcctc gggcagggcg gccagggcct cgcccagcgc ctggcgcagc ggcgcgcgcc
     2161 agccggcgcc ggcgccctcg cctgccgcca gcgcggcaaa atccaccccg cgcagccgcc
     2221 gcgccagcgc cgccggcacc aggaaatggc gctccacgaa gcgggccagg ctgtctgcca
     2281 gccggtcgcg gttggtgggg atgatcgcgg tgtgggggat gggcaggccc agcgggcggc
     2341 ggaacagcgc cgtcacggca aaccagtcgg cgaggccgcc caccagcgct gcctcggcaa
     2401 aggccagcgg ccattgccac aagggatgcg cggccacctg ccgccggccg atcgcaaaca
     2461 ccagccccat cgccaccagc aggccgcccg ccagccagcg cagcggccgg gccggatcag
     2521 gggtgccgct catgccggat gggaaacgca tggggctgct atgccggcgc gcggcgccct
     2581 tgcgcaaggg ccctcaacgc aaaaggcctc acccggccag cgccggatga gacccccgaa
     2641 cacgcagccc ccggcgttca gtatttgaac tggcgctcgt agagatcgcg atagtgctgg
     2701 atgcgggtga cgcgcaggcc ctgcatgccc gaacggtcca ccgcccgctg ccacgaggcg
     2761 aactcctcca gcgtcagccc atagcgctcg cacacctcgt cgatggtgag caacccgccg
     2821 ttcaccgcgg ccaccacttc tgccttgcgg cgcaccaccc agcgggtggt ggtggggggc
     2881 ggcagggatt ccagcgtgag cggttcaccc agggggccga tcaccatggc tggccggatt
     2941 ttctgattct cgatcattgc aaacctcgat cgccggtcgg aagggggccg gcagagccca
     3001 aggaaatgcc gggattggtt ttgaaatcgc ctaaagcaac agggttaacg gcagatgagc
     3061 cgcgccccct cgccgccggc catgcccgat cctgcgggat aaaccggtgt ccggcctgct
     3121 gtgccgcggt tttgcggccg gtggagatcg ccccgctggc gccaattccg gggtcttgtg
     3181 tagagatctt gtccatgcgc cccttgtagc ctggcaatgt caaaagcagg taaaccatgc
     3241 ttttaccaga tggttacaat ggttaacaag gtgggtggca agccttccgc aacacacccc
     3301 attggccctg cccccgccgt taacccaaag tggcaagatt atgcccggca cacggcaggc
     3361 aatggccggc ggttaaccat tgagcgccgc cgccggccat caccccctcg cgcgcttttg
     3421 catggccgcc gccgctgggc tatggcgggc gcatgtccag cttctgccag cccccgatcc
     3481 ttgccgacgg cccgcccctg ccccctgcca ccctggcgcc agaggcattg gccggcctgt
     3541 tccagctggc cgcgccgctg gccgcgcggc gggtggtggt ggcgatgtcg ggcggggtgg
     3601 attcctcggt ggtggcggcc ctggcggcgg ccagcggcgc cgaggtgatc ggcatcaccc
     3661 tgcagcttta cgatcacggc gaggcggtga agcgcaaggg cgcctgctgc gcgggcgatg
     3721 atatccgcga tgcgcgcgcc gtggccgacc ggctgggcat cgcccattac gtgttcgacc
     3781 atgagtcgtc cttccgcgag gaggtggtgg agcgctttgc cgaggaatat ctggccgggc
     3841 gcacgccgat cccctgcatc cgctgcaaca tggggcccaa attcaccgat ctgttcgcca
     3901 tggcgcgcga cctcggcgcc gattgcctgg ccaccggcca ttatgtgcgc cgcgtggcag
     3961 ggcccgccgg cgccg
//
LOCUS       DASRYE010000005        11686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_230102,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000005 DASRYE010000000
VERSION     DASRYE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230102"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFF98_00100"
     CDS             <1..352
                     /locus_tag="VFF98_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HZU62054.1"
                     /translation="RFRAPDACHGFRMNYINPLTGGPAMPTISTAMRLLPKGFVSAPY
                     RSTAGTVFSVVEGTGVARVGEQVFSLAPRDIFVVPSWFPVTFEAAEDLVLFTYSDQIV
                     QEKLDFFREKRGND"
     gene            345..1034
                     /locus_tag="VFF98_00105"
     CDS             345..1034
                     /locus_tag="VFF98_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU62055.1"
                     /translation="MTEFVFAPAPVMAVPVEGSDKAFPVRRIYCVGRNYLDHIREMGQ
                     DERQPPFFFQKPTDAIVLDGAAIAYPVETRDFQFEIELVLAIGKGGANIAVEDAAAHV
                     FGTAVGIDFTRRDLQLRARESGRPWEPGKSFDASAPIGAIVPLGAGGLPAQGDITLDV
                     NGEQRQKGDLAQLIWNCAEIVAQLSRQYRLEPGDLIYTGTPAGVGPVVAGDRITGKVA
                     GLPDLSVAIMG"
     gene            1136..1519
                     /locus_tag="VFF98_00110"
     CDS             1136..1519
                     /locus_tag="VFF98_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU62056.1"
                     /translation="MTNKIHMGLALLGLAAAQPALAQNGAQNGAHGKALFAQCRACHT
                     DVKGARSTVGPNLSGVVGRKAGSLAGFAYSPAMKAAGIVWTPARIAAYLAAPARAVPG
                     NRMGYAGMASPGDRADLVAYLESLR"
     gene            complement(1498..2724)
                     /locus_tag="VFF98_00115"
     CDS             complement(1498..2724)
                     /locus_tag="VFF98_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62057.1"
                     /translation="MPAQPAIGPVEFVALIAMLMAMSALGVDMMLPALPDIGQALQAP
                     GAGQQQFIITLFGAGFGASQICHGPLIDRFGRRRVLLWALVAYALLNAVAALSMRFGF
                     LLAIRFLSGSAVSGVRVAALAILRDCHAGQAMARMLSLAFMVFMIFPILAPAFGAGIL
                     LIGNWRSIFWCLAVLSLAIFGWVALRLPETLLPEHRRPIRMGAILRGWQATLGHRLSL
                     GYTLATMCLTGAFYGYLGSIQPIMAQCFKRADLLLPVFATSAGVMMAANLLNARLVMR
                     LGARRLSTAAIAIMTAAASLQLLLALQGLETLVLFTLLQALMLASFSLSSANFAAIAM
                     EPMGAVAGTASGLQGFIALTFGAALGALVGHAFDGTIVPLASGFLLAALATLAITAMA
                     GRKRPGPPADQRRLSR"
     gene            complement(2775..3977)
                     /locus_tag="VFF98_00120"
     CDS             complement(2775..3977)
                     /locus_tag="VFF98_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62058.1"
                     /translation="MMNIHTASNAELETRLIERTRALIPVLQARARASEAACSLAAET
                     VADFREAGLFRILQPRSAGGYGLSPHVLCKVVFEAGRGDMSAAWVLFVLALHQYEVSL
                     MDARAQQDIWGSAADALVASSVAPFGRLERVEGGYRLSGRWRFSSGIDHAQWVGVGGL
                     VEVPGRPGKDFRMSLLPVGAVRIDHASWRPFALAGTGSKDFDVDGAFVPEHMTFSLID
                     AHDMIGQEALPASNRYPFWVVFNAVLGAAIIGGTMGGVDEAQEQLRDRLGSADNGEGK
                     QKASDDPFVRARIARARLIVRSAQARYAAIFAEMDEHIARGEPIPVASRMHYIAEFAQ
                     CGRDCEEAMLTLYKTTGGRGLSLDNRMQAILRNVLGGANHIAMNLDPMLQNLGGLLLG
                     GAIPKVLC"
     gene            complement(4104..6473)
                     /locus_tag="VFF98_00125"
     CDS             complement(4104..6473)
                     /locus_tag="VFF98_00125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HZU62059.1"
                     /translation="MTRLIVAGGRLFGLLQGLAGLFLVGNGAQLLARGGSAYYLLAGL
                     ILLGVALGCLRGKAWGGWLYQAFLAGTIGWALWETGADFWPFYARTFLFLVMGIPAGL
                     LLAGLAGGRAPRRRLGGLLIAGIEGLGALAMLLAMFQPHDAIRPDPALPPLAQPAPSG
                     AIHTQDWAHYGNTPEGERHADADQITRANVASLRQAWVYHTGDIPRDYSGTGSEDQNT
                     PLFVAGTVYVCTPHNTVIALDGDTGAERWKYRIDVGDPKKWVRCRGLGYADSAALPAA
                     ARAAGPAPGHADCPRRIFVNTARARLLALDAQTGRPCADFGEGGTVDLTAGIGKVPAD
                     LYKPTSGPTVAGRVVVVGSYVNDNVSTDMPSGVVRAYDVRDGRLVWAFDTGRPEDHGP
                     PRPGTTYTRGSPNVWAPMSYDPATNLVFLPTGNASPDIWGGNRTPAEDRFSSSVVALD
                     AATGRLRWSFQTVHHDLWDYDVPMQPTLTAFPAAGGRLVPAVVFGTKLGQIYVLDRAT
                     GRPLTRVEERPVPAGTISGERYAPRQPLSTGMPAIGTQTLREADMWGMTPFDQLLCRI
                     AFRGMRYTGLFDPPGLDRSLTFPGALGGMNWGGVSVDPRSHTLFVNDLRLAQWYQLFP
                     RRPGVADDTLPFPMSGAPVSLEKGRFFSVFGVPCQKPPYGTMTAIDLATRKIRWQIPL
                     GTIEDTGPLGIRTGLPLPIGMPTIGGSLSTGGGLVFFAGTQDYYLRALDSASGRELWK
                     QRLPVGSQGGPMTYVSPRTGTQYVLITAGGARQSPDRGDYVIAYKLQGR"
     gene            complement(6470..7564)
                     /locus_tag="VFF98_00130"
     CDS             complement(6470..7564)
                     /locus_tag="VFF98_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZU62060.1"
                     /translation="MMKRRAILLALAALAVRQPASAETPGPASGVTTLRLHQMLPADA
                     AFTRLGLQAWARAVEQDAGGRLRIEIHSGMELGGKPADLYDQARDGTVDLSWVVLGYT
                     PGRFPATTTFELPFMVRSARATSQAFQSFCTLYCASAFGDVHVIAWHTHGPALIHASR
                     PIASLDDLRGLRLRPGSAAIGQFLAAAGATTLAMPATQVYEALRSGQIDATTIPWSSE
                     VNTSKPASDILHFHTAAGGPRGLYTQTFALVMNQAAYRRLPAPLRAVIDRHSGLAAAR
                     LFGQAMDESDRLGRTAALAHGDRLITLGPAETARWRQAAQPVIAAWRTAMQARGLEAG
                     RLLARANALLDRAGRNEARHNEAQHNESGR"
     gene            7719..8156
                     /locus_tag="VFF98_00135"
     CDS             7719..8156
                     /locus_tag="VFF98_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU62061.1"
                     /translation="MAITTTAPDTTSQSLLEQIDTGVLLRIHAGAFFGPQIYYKIWSF
                     RGITQFLEMAGFYPAPVWVVLAGVVETLVAIGLVFNIGAKYAALAAIGVLAVAAGAVV
                     RVRGLGWLFNHGGIEYHVFWLLICFYVFVNEWQKRPGLLPRAS"
     gene            complement(8232..9677)
                     /locus_tag="VFF98_00140"
     CDS             complement(8232..9677)
                     /locus_tag="VFF98_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU62062.1"
                     /translation="MYSTQMLIAGQPAPMHGPAFERANPASGKPVNRLTGASPADASA
                     AADAAAAAFAPWSRTGPGMRRDLLLRAEQLMHAREEAIVAAMQAELGAAIGWARFNIH
                     VATGILRECAALTTAITGRTLPSDIPGLTAMTHRRPCGVILSIAPWNGPVILAARAIA
                     MPLACGNTVVLKGSELCPQTHRLVVDCFIDAGLPAGVLNYVVNAPEDAAAVVVTLIAH
                     PAVRRVNFTGSSHVGRIIAEQAGRHLKPCLLELGGKSPLIVLEDADIEEAAHAACFGA
                     FINSGQVCMATDRILVDERVADRFVASLRQRAAALSVGDGRHAQLGPLAMAGAADRLA
                     DLVAEAVALGAQCVLQAPRDGQIFGPVILDRVTPAMRIYHEESFGPIAPISRFTTVEQ
                     AIAMANDSPYGLAASVFGRDIGRALALASQLDAGNRHINMATVHDQPHMPFGGLKDSG
                     YGRFNGPEAIAEFTDTCLTTLRTEPAAHYPF"
     gene            complement(9693..10682)
                     /locus_tag="VFF98_00145"
     CDS             complement(9693..10682)
                     /locus_tag="VFF98_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZU62063.1"
                     /translation="MANPPKLRIDASGIHGVWPILPTPSKPDASDWRAQDTVDLDETA
                     RVVEALIAAGVNGLMSLGTYGEAHSLLWAEKKAFLGCVIETVAGRIPFFAGTTALNTR
                     EVVEQTRAVHDMGASGTMLGVPMWCRADLPTAVQFFHDVTEACPDTALSIYANSEAFK
                     FEFSRPFWAAMGQLPQAVSCKYLGIGLLAADLELAPNLRFLPTECDYYAAARMAPERM
                     TAFWSSAALCGPAPQIALRDRVAAARQSGDWTAAKEVADAMRQADLGLFPRGEFSEFS
                     KFNVGLEKGRMDAAGWMRAGPPRPPYHVIPEEYLAGAARSGKAFAALHARYTV"
     gene            10876..>11686
                     /locus_tag="VFF98_00150"
     CDS             10876..>11686
                     /locus_tag="VFF98_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU62064.1"
                     /translation="MKFLSFEHEGQARWGALAGNGRIVDLTERYPGLRAALAAGPLAG
                     LRVEAAEATLDVEAVRLLPVIPDPARILCIGVNYHSHREEMQRAHSAQPTVFLRLADT
                     QVGHGQALLRPRESEQLDYEGEIAVVIGKAGRRIAPENAWDHVAGYAPYCDATIRDWQ
                     RHGTAQQWTPGKNFPGTGGFGPWLVTRDEIPDGSDLTLETRLNGQIVQQTSMGLLIFS
                     IPELIAYCSTFTRLEPGDVIATGTPGGVGGKRQPPLWMKPGDILEVEVGGVG"
BASE COUNT         1925 a         3852 c         4123 g         1786 t
ORIGIN      
        1 gcgctttcgc gcgcccgatg cctgccatgg cttccgcatg aactacatca acccgctgac
       61 cggcggcccg gccatgccga cgatctccac cgccatgcgc ctgctgccca aaggcttcgt
      121 ctcggcgccc tatcgctcga cggcgggcac ggtgttcagc gtggtggaag gcaccggcgt
      181 tgcccgtgtg ggcgagcagg tcttttccct cgcgcccagg gatatcttcg tcgtgcccag
      241 ctggttcccc gtcaccttcg aggccgccga ggatctcgtc ctcttcacct actcagacca
      301 gatcgtacag gaaaaactcg atttcttccg ggagaagcgt ggcaatgact gagttcgtat
      361 tcgcgcctgc gcccgtgatg gccgtgcccg ttgagggcag cgacaaggcc tttccggtgc
      421 gccgcatcta ttgcgtgggc cgcaactatc tcgatcatat ccgcgagatg gggcaagacg
      481 agcgccagcc gcccttcttc ttccagaagc cgaccgatgc catcgtgctc gatggcgctg
      541 ccattgccta tccggtcgag acgagggatt tccagttcga gatcgagctg gtgctggcca
      601 tcggcaaagg cggggccaac atcgcggtcg aggatgcggc tgcccatgtc tttggcaccg
      661 cggtcggcat cgatttcacc cggcgcgatc ttcagctcag ggcgcgcgag agcggccgcc
      721 cgtgggagcc gggcaaatcc ttcgatgcct cggcccccat cggcgccatc gtgccgctgg
      781 gcgcgggcgg gctgcctgcg cagggcgaca tcactctcga tgtcaatggc gagcagcgcc
      841 agaagggcga tctggcccag ctgatctgga attgcgccga gatcgtcgcc cagctgtcgc
      901 ggcaatatcg tctcgagccg ggcgatctga tctacaccgg cacgccggcc ggtgtcggcc
      961 cggtggtggc aggagacagg atcaccggca aggtggcagg cctgcccgac ctgtcggtcg
     1021 ccattatggg gtgaatggtg gggtgagcgg ggcggcccgc tgcccaaagc tgatccgcat
     1081 cggcctcaac cgcttccacg ctggtgcggg ccgatgcact gcgaaggaat atccgatgac
     1141 gaacaagatc cacatgggtc tggccctgct gggcctggcc gccgcgcagc ccgcgctggc
     1201 ccagaacggg gcccagaacg gggcccacgg caaggcgctc ttcgcccagt gcagggcctg
     1261 ccacaccgat gtgaagggcg cccgctcgac ggttggcccc aacctttccg gcgtggttgg
     1321 ccgcaaggct ggcagccttg ccggttttgc ctattccccg gccatgaagg cggccggcat
     1381 cgtctggacg ccggccagga tcgcggccta tctggcagcg cccgccaggg ccgtgccggg
     1441 caaccgcatg ggctatgccg gcatggccag ccccggcgat cgggccgatc ttgtggccta
     1501 tctggaaagc ctgcgctgat cggcaggggg ccccgggcgt tttctgccag ccattgcggt
     1561 gatcgccagg gtggcaaggg ctgccagcag aaatccgctc gccagcggca cgatggtgcc
     1621 atcgaaggca tggccgacca gcgcgcccag cgcagcgccg aaggtgagcg cgatgaagcc
     1681 ctgcagcccg gatgcggtgc ccgccacagc ccccatcggc tccatggcaa tcgccgcgaa
     1741 attggcgctg gagagggaaa agctcgccag catcagcgct tgcagcaggg tgaagagcac
     1801 aagcgtttcc agcccctgca gcgcaagcag cagttgcagg ctggcggccg cggtcatgat
     1861 ggcgatggcc gcggtgctca accgcctggc gcccagccgc atcaccagcc gcgcgttgag
     1921 cagattggcc gccatcatga caccggcgct ggtggcaaac accggcagca gcaggtccgc
     1981 ccgtttgaag cattgtgcca tgatcggctg gatcgagccc agatagccgt aaaaggcccc
     2041 ggtcaggcac atcgttgcaa gcgtgtagcc cagcgacagc cgatggccca gcgtggcctg
     2101 ccagccacgc aggatggcgc ccatgcggat cggccgccgg tgttctggca acagcgtttc
     2161 gggcaggcgc agggccaccc agccgaaaat ggccagcgaa agcacggcca ggcaccagaa
     2221 gatgctgcgc cagttgccga tcagcaggat gccggcgcca aaggccgggg ccaggatcgg
     2281 aaagatcatg aacaccatga aggccagcga cagcatccgc gccatggcct gcccggcgtg
     2341 gcaatcgcgc aggatcgcca gcgctgccac ccgcacgccc gataccgcgc tgccggagag
     2401 aaagcggatg gcaagcagga agccgaagcg catcgacagg gcggccaccg cattgagcag
     2461 cgcataggcc accagcgccc acagcagcac gcggcgccgg ccgaaacggt cgatcagcgg
     2521 gccgtggcag atctggctgg cgccaaagcc ggcgccaaac agggtgatga tgaattgctg
     2581 ctgcccggcc ccgggcgcct gcagcgcctg gccgatatcg ggcagggcgg gcagcatcat
     2641 gtcgaccccc agggccgaca tggccatcag catggcgatc agcgcgacga attccaccgg
     2701 gccgatggcc ggttgggcgg gcatcgccgg catgggcgcc gatgcagggg cggggggcat
     2761 cgccgcccgc cggctcagca cagcaccttg gggatggccc cgcccagcag cagcccgccc
     2821 aggttctgca gcatcggatc gaggttcatg gcaatgtggt tggccccgcc cagcacgttg
     2881 cgcaagatcg cctgcatgcg gttgtcgagc gacaggccgc ggcctccggt ggtcttgtaa
     2941 agcgtcagca tggcttcctc gcaatcgcgg ccgcattgcg cgaattcggc gatgtagtgc
     3001 atgcgcgatg ccaccggaat cggctcgccc cgggcgatgt gctcgtccat ttcggcgaag
     3061 atggcggcat agcgggcctg ggcgctgcgc acgatcagcc gggcgcgggc gatgcgggca
     3121 cgcacgaagg gatcgtcgct ggctttctgc ttgccttcgc cattgtcggc cgaacccagc
     3181 ctgtcgcgca gttgctcctg cgcctcgtcg acaccgccca tcgtgccgcc gatgatcgcg
     3241 gcgcccagca ctgcgttgaa caccacccag aagggatagc ggttgctggc tggcagcgcc
     3301 tcctggccga tcatgtcgtg ggcatcgatc aggctgaagg tcatgtgctc gggcacgaag
     3361 gcgccatcca cgtcgaaatc cttgctgccg gtgccggcca gggcgaaagg gcgccacgag
     3421 gcatgatcga tccgcaccgc gccaaccggc agcagcgaca tgcggaaatc cttgccgggg
     3481 cggccgggca cttccaccag cccgccaacg cccacccact gggcatgatc gatgccgctg
     3541 gaaaagcgcc agcgcccgct cagccgatag cccccctcga ccctctccag ccggccgaac
     3601 ggggccaccg aggaggccac cagcgcatcg gcggcgctgc cccagatgtc ctgctgcgcg
     3661 cgcgcgtcca tcaggctcac ctcatactgg tggagcgcca gcacgaacag cacccaggcc
     3721 gccgacatgt cgccgcgccc ggcctcgaag acaaccttgc acagcacatg gggcgaaagg
     3781 ccatagccgc cggccgaacg gggctggagg atccggaaca ggcccgcctc gcgaaaatcg
     3841 gcaacggttt cggcggccag cgaacaggcc gcctcgctgg ccctggcgcg ggcctgcaga
     3901 accgggatca gggcgcgggt gcgttcgatc agccgggttt caagctcggc attgctggcg
     3961 gtgtggatat tcatcatgcg tttctctcca tgcccggcat gggccgtgcg ggtgaaccgg
     4021 gcaaaggcta ggcgggaagg ggcgggccgg gcttcatcgg ttcggatgaa ggctggcgca
     4081 atttcgcccg ggcgaggggg cggctatcgg ccctgcagct tgtaggcgat cacgtaatcg
     4141 ccccgatcgg gagactggcg cgcgccgccg gcggtgatca gcacatattg ggtgcccgtg
     4201 cgcggcgaca cataggtcat cggcccgccc tggctgccca ccggcaggcg ctgcttccac
     4261 aattcccgcc cgctggcgct gtcgagcgcg cgcagataat agtcctgcgt gccggcgaag
     4321 aacacaaggc cgccgccggt ggaaagcgag ccgccaatgg tgggcatgcc gatcggcagg
     4381 ggcaggccgg ttctgatgcc cagcggccct gtatcctcga tcgtgccgag cgggatctgc
     4441 cagcggatct tgcgggtggc cagatcgatg gcggtcatcg tgccataggg gggcttctgg
     4501 cagggcacgc caaagaccga gaagaaccgc cccttttcca gcgacaccgg ggcgccgctc
     4561 atcgggaagg gcagggtgtc gtcggcaacg ccgggccggc ggggaaacag ctgataccat
     4621 tgcgccagcc gcaaatcgtt gacgaaaagg gtgtggctgc gcggatcaac ggagacgccg
     4681 ccccagttca tgccgcccag cgcgccgggg aaggtcagcg atctgtcgag gccgggggga
     4741 tcgaacaggc cggtatagcg catgcccctg aaggcgatgc ggcacagcag ctggtcgaac
     4801 ggggtcatcc cccacatgtc ggcctcgcgc agggtttgcg tgccgatggc gggcatgccg
     4861 gtcgacaggg gctggcgcgg cgcatagcgt tccccgctta tcgtgcctgc cggcaccggg
     4921 cgctcctcca cccgggtcaa cgggcggccg gtggcgcgat cgagcacata gatctggccc
     4981 agcttggtgc cgaacaccac ggccggcacc agccgcccgc cggccgcggg aaaggcggtg
     5041 agggtgggct gcatcggcac gtcataatcc cacaggtcgt ggtgcacggt ctggaaggac
     5101 cagcgcaggc ggcccgtggc ggcatcgagc gcaacgacgg acgagctgaa gcggtcttcg
     5161 gccggggtac gattgccgcc ccagatatcg ggcgaggcat tgccggtggg cagaaacacc
     5221 agattggtgg ccggatcata cgacatgggc gcccagacat tgggcgagcc gcgcgtatag
     5281 gtcgttcccg ggcggggcgg gccgtgatcc tccgggcggc cggtgtcgaa ggcccagacg
     5341 agccggccat cgcgcacatc ataggcccgc accacgcccg agggcatgtc ggtggagacg
     5401 ttgtcgttga catagctgcc gaccaccacc acccggccgg ccacggtggg gccggatgtg
     5461 ggtttgtaga ggtctgccgg caccttgccg atccctgctg tcagatcgac cgtgccgccc
     5521 tcgccgaaat ccgcgcaggg cctgccggtt tgcgcatcaa gggcaagcag ccgggccctt
     5581 gcggtgttga cgaagatccg ccgggggcaa tccgcatggc caggcgcggg gccggcggcc
     5641 cttgccgcgg cgggaagcgc ggcgctgtcg gcatagccca gcccgcggca gcgcacccat
     5701 ttcttggggt cgcccacgtc gatccggtac ttccagcgct cggccccggt gtcgccatcc
     5761 agcgcgatga cggtgttgtg cggggtgcag acatagaccg tgccggccac gaacagcggg
     5821 gtgttctggt cctcgctgcc ggtgccgctg taatcgcggg ggatatcgcc ggtgtgatag
     5881 acccatgcct gccgcaggct ggcgacattg gcgcgggtga tctggtcggc atcggcgtgg
     5941 cgctcgccct cgggggtgtt gccgtaatgg gcccagtctt gcgtgtggat ggcgccggaa
     6001 ggggccggct gggcaagggg cggcagcgcc ggatcaggcc ggatcgcatc atgaggctgg
     6061 aacattgcca gcaacatggc cagggcgccc agcccttcga tgccggcgat cagcaggccg
     6121 ccaagccgcc ggcgcggggc ccgcccgcca gccagcccgg ccagcagcag gccggcagga
     6181 atgcccatca ccagaaagag gaaggtgcgg gcatagaacg gccagaaatc agcgccggtt
     6241 tcccacagcg cccagccgat cgtgccggca aggaatgcct gatagagcca gccgccccag
     6301 gccttgccac gcaggcagcc aagggcaacc cccagcagga tcaaccccgc cagcagataa
     6361 taggcactgc ccccacgggc cagcaactgc gccccgttcc ccaccagaaa caggcccgcc
     6421 agcccctgca gcaggccgaa caggcgcccg cccgccacga tcagccgcgt caccggccac
     6481 tctcgttgtg ctgtgcctca ttgtgccggg cctcgttgcg gccagccctg tccagcagcg
     6541 cgttggcgcg ggcaagcagc ctgcctgcct ccagccccct ggcctgcatc gccgtgcgcc
     6601 aggcggcgat caccggctgt gccgcctgcc gccagcgcgc ggtttctgca gggccaagcg
     6661 tgatcaggcg atcgccatgg gccagcgctg ccgtgcggcc caggcgatca ctctcgtcca
     6721 tcgcctggcc gaacaggcgg gcggcggcaa ggccggaatg gcgatcgatc actgcgcgca
     6781 agggggcggg cagccggcga taggccgcct ggttcatcac cagcgcgaag gtttgcgtgt
     6841 agagcccgcg cgggccgccg gccgcggtgt gaaaatgcag gatatcgctg gcaggcttgc
     6901 tggtgttcac ttcggaggac caggggatgg tggttgcatc gatctggccc gatcgcagcg
     6961 cctcatagac ctgggtggcg ggcatcgcca gggtggtggc gccggcggcc gcgaggaact
     7021 ggccgatggc ggccgaaccg gggcgcagcc gcagcccgcg cagatcatcg aggctggcga
     7081 tggggcggct ggcgtggatc agcgcggggc catgcgtgtg ccaggcgatg acgtgaacat
     7141 cgccaaaagc gctggcgcag taaagcgtgc agaagctctg gaaggcctgc gaggtggcgc
     7201 gggccgagcg gaccatgaac ggcagctcga aggtcgtcgt cgcgggaaag cggccgggcg
     7261 tgtagcccag caccacccac gacagatcga ccgtgccatc gcgcgcctga tcgtagaggt
     7321 cggcgggctt gccgcccagt tccatgccgc tgtggatctc gatcctcagc ctgccgccgg
     7381 catcctgctc caccgcgcgg gcccatgcct gcagccccag acgggtgaag gccgcatcgg
     7441 ccggcagcat ctggtgcagg cgcagggtgg tcacgccgga ggccgggccg ggcgtctcgg
     7501 cgctggcggg ctgccgcacc gccagcgcgg ccagcgccag caggatggct cgccgtttca
     7561 tcatgccatg agatccttct gtcgtgttca tctgccctgt ggcgataggg ggatgggcgg
     7621 gcgctgcgcc tcatctgatc ggatgaggcc cctgaagggc tgtggtgcga aggctgcggg
     7681 caagcgggaa agccccgcca gatccaaggg agagcatgat ggcgatcacg acaaccgcgc
     7741 cagacaccac aagccaaagc ctgctggaac agatcgacac gggggtgctg ctgagaattc
     7801 acgccggcgc gtttttcggg ccgcagatct actacaagat ctggagcttc cggggcatta
     7861 cccagtttct cgagatggcc ggcttctatc cggccccggt gtgggtggtg ctggcggggg
     7921 tggtggaaac gctggtggcc atcgggctgg tgttcaacat cggcgcaaaa tatgcggcgc
     7981 tggcggcgat tggcgtgctg gcggtggcgg cgggcgcggt ggtcagggtg cgtggccttg
     8041 gctggctgtt caaccacggc ggcatcgaat atcatgtgtt ctggctgctg atctgtttct
     8101 atgtctttgt gaatgaatgg caaaagcgcc ccggcctgct gcctcgggcc agctagtcgg
     8161 ggcatggctt gcgcccttgt gccggctggc gcaagggcgc aaggggggac ggttttccgc
     8221 ttcaggcggg cctagaacgg ataatgcgcg gccggttccg tgcgcagcgt ggtcaggcag
     8281 gtgtcggtga attcggcgat ggcctcgggc ccgttgaagc gcccgtagcc gctgtctttc
     8341 agcccgccga agggcatgtg cggctgatcg tgcaccgtgg ccatgttgat gtggcgattg
     8401 ccggcatcaa gctggctggc cagcgccagc gcccgcccga tatcacgccc gaacaccgag
     8461 gcggccaggc cataggggct gtcgttggcc atggcgatgg cttgttcgac agtggtgaag
     8521 cggctgatcg gggcgatcgg gccgaagctt tcctcgtgat agatgcgcat tgccggtgtc
     8581 acgcggtcga ggatgacggg gccgaatatc tggccatcgc gcggtgcttg cagaacgcat
     8641 tgcgccccca gcgccacagc ttcggccacc agatccgcca gcctgtctgc cgcgccggcc
     8701 atggccagcg ggcccagctg ggcatggcgg ccatcgccca ccgagagggc cgccgcgcgc
     8761 tggcggaggc tggccacgaa gcggtcggcc acgcgctcgt ccaccagaat gcggtctgtc
     8821 gccatgcaga cctgcccgct gttgatgaag gcgccgaagc aggcagcgtg cgccgcttcc
     8881 tcgatatcgg catcctccag cacgatcagc ggcgatttgc cgcccagttc cagcaggcag
     8941 ggcttcaggt ggcgcccggc ctgctcggcg atgatccgcc cgacatggct cgacccggtg
     9001 aaattgacgc gccgcaccgc cggatgggca ataagcgtga cgaccaccgc tgcggcatct
     9061 tccggcgcgt tgacgacata gttgagcacg ccggccggca gccctgcgtc gatgaagcaa
     9121 tcgaccacca ggcggtgcgt ctgcgggcac agttccgatc ccttgagcac gaccgtgttg
     9181 ccgcaggcca gcggcatggc gatggcgcgc gcggccagga tcaccgggcc attccacggc
     9241 gcgatgctga ggatcacgcc gcaggggcgg cgatgggtca tcgccgtgag gccgggaatg
     9301 tcggagggca gggtgcgccc ggtgatggcc gttgtcagcg ccgcgcattc gcgcaggatg
     9361 ccggtggcga catggatgtt gaaccgggcc cagccgatgg ccgcgcccag ttccgcctgc
     9421 atggccgcca cgatcgcctc ctcgcgcgca tgcatcagct gctcggcgcg cagcagcagg
     9481 tcgcgccgca tgccgggccc cgtgcgggac catggcgcga aggcggcggc ggcggcatcg
     9541 gcagcagcgc tcgcatctgc cggcgaggcg ccggtcagcc ggttgaccgg cttgccgctg
     9601 gcagggttcg cgcgttcgaa ggcggggcca tgcatggggg ccggctggcc ggcgatgagc
     9661 atttgcgtgg aatacatcct cgtccctccc gctcagacgg tgtagcgcgc atgcagggcg
     9721 gcaaaggcct tgcccgagcg tgcggccccg gcgagatatt cttcgggaat gacatgatag
     9781 ggcgggcgag gggggccggc gcgcatccat ccggcggcgt ccatccggcc cttttccagc
     9841 cccacgttga atttcgagaa ttccgagaac tcgccgcggg gaaacaggcc cagatccgcc
     9901 tggcgcatgg catcggccac ttccttggcg gctgtccagt cgccgctctg cctggcggcg
     9961 gccacccggt cgcgcagggc gatctgcggc gccgggccgc acagcgccgc agatgaccag
    10021 aaggcggtca tccgctcggg cgccatgcgc gcggcggcat aataatcgca ttcggtgggc
    10081 aggaagcgca ggttcggggc cagttccaga tccgcggcca gcaggccgat gcccagatat
    10141 ttgcacgaca ccgcttgcgg caactgcccc atcgccgccc agaacgggcg cgagaactcg
    10201 aatttgaacg cctcgctgtt ggcatagatt gacagggcgg tgtcggggca ggcttcggtc
    10261 acatcgtgga agaactgcac ggccgtgggc agatcggcgc ggcaccacat cggcacgccc
    10321 agcatggtgc cggaggcgcc catgtcgtgc accgcgcggg tctgttccac cacctcccgg
    10381 gtgttgagcg cggtggtgcc ggcaaagaag ggaatgcggc cggcgaccgt ttcgatcacg
    10441 cagcccagga aggccttttt ctccgcccac agcaggctgt gcgcctcgcc ataggtgcca
    10501 aggctcatca gcccgttcac gcccgcagcg atcagcgctt ccaccacgcg ggcggtttcg
    10561 tcgagatcga cagtatcctg cgcgcgccag tcggaggcgt ccggtttcga tggcgtgggc
    10621 aggatcggcc agacgccgtg aatgccgctg gcatcgatac gcagtttggg tgggtttgcc
    10681 atgtcctctc ctgtgggtgc cgggcaggcg gatcggcccg ccgggggaat gtggcggcca
    10741 gactaggagg caggcgcgcg ccggccttca tccgatcaga tgatgcgtgg cgcagggcag
    10801 ggccctaacc atccggggca tcggcctgtg atgcgcaagg ctatcggcaa aaagccgcag
    10861 atatgggaaa agggcatgaa atttctgagc tttgagcatg aaggccaggc gcgctggggg
    10921 gcgctggccg gaaacgggcg gatcgtggat ctgacggagc gctatccggg cctgcgggcg
    10981 gctttggcgg ccggccccct tgccgggctg cgcgtggagg cggccgaggc gacccttgat
    11041 gtcgaggcgg tgcggctgct gccggtcatt cccgatccgg ccaggatcct gtgcatcggc
    11101 gtcaattacc acagccaccg ggaggaaatg caacgggcgc acagcgccca gcccaccgtt
    11161 ttcctccgcc tggccgatac gcaggttggc catgggcaag cgctgctgcg cccgcgcgaa
    11221 tccgaacagc ttgattacga aggcgagatc gcggtggtga tcggcaaggc cgggcgccgc
    11281 atcgcgcccg agaatgcgtg ggaccatgtc gccggctatg ccccctattg cgatgccacc
    11341 atccgcgact ggcagcgcca tggcaccgcc cagcagtgga cgcccggcaa gaacttcccc
    11401 ggcaccggcg gctttggccc gtggctggtg acgcgcgacg agatccccga tggcagcgac
    11461 ctgacgctgg aaacgcgcct caacggccag atcgtgcagc agaccagcat ggggctgctc
    11521 atcttcagca tccctgaact gatcgcctat tgctcgacct tcacgcggct ggagccgggc
    11581 gatgtcatcg ccaccggcac gccgggcggg gtgggcggca agcgccagcc gcccttgtgg
    11641 atgaagccgg gcgacattct ggaagtcgag gtcggcggtg tcggca
//
LOCUS       DASRYE010000006         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_230122,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000006 DASRYE010000000
VERSION     DASRYE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230122"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFF98_00155"
     CDS             <1..373
                     /locus_tag="VFF98_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62065.1"
                     /translation="RFRAPDACHGFRMNYINPLTGGPAMPTISTAMRLLPKGFVSAPY
                     RSTAGTVFSVVEGTGVARVGEQAFPLAPRDIFVVPSWFPVTIEAGEDLVLFTYSDQIV
                     QEKLDFFREKRGDGPGDSLAG"
     gene            416..937
                     /locus_tag="VFF98_00160"
     CDS             416..937
                     /locus_tag="VFF98_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZU62066.1"
                     /translation="MEELQARFRATMAHYPAGVVIVTTRDGHGRLQGFTATSFSSLSL
                     APPSILVCLAHAAFCYEAFVTSRHFAINFVGAGHDGLALRFARRGEDKFAGVPVRLTE
                     QGVPLLCEAAATLECATDSHVVSGDHAILVGNVLDAAIAEGSDVLVHYQRRFGHVRFA
                     RAGGDPADHGAVR"
     gene            939..>2561
                     /locus_tag="VFF98_00165"
     CDS             939..>2561
                     /locus_tag="VFF98_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZU62067.1"
                     /translation="MAAEAPAGRAGGWMRRLAMPIGGLIALFGLCIGGAGAWLLFLGG
                     SWFYAAFGPACCVCGVLVARRRPAGVWLWAVIFIATWGWALGEVGADFWPLVPRVAPV
                     MAMGLAILPVAPGLFGGRAGWVAWPLAGGMGLAVVAGIYGMLLPHGAIRPSAQAAGWV
                     RGLPAAAGPEQDWRQYGRTADGQRYAPFDQINAGNVARLKRAWTYRTGDIAQGPLKDE
                     NTPLQVGDTLYVCTPKDRVIALDGDTGQQKWVFDARATGPEFVLCRSLGYADLARGGH
                     GEGRPAAPCTQRLFLTTTDGRLMALDALSGKPCAAFGKAGTVDLRAGLGTFDPRFYFS
                     NSGPLVIENALVVVGARVKDNQSVAEPSGVVRAFDAHSGALVWAWDLGRPDRSAAPAP
                     GKTYTPGTPNMWTTLTYDPALGLVYLPLGNATPDFWGGRRRQVDEAYTDAVVAVDYRT
                     GKERWHYRTMNHDLWDYDLPAPGTLYDMPDGKGGTLPALIQITKRGEIFVLDRRNGRP
                     ITPVVQRPVPTGPAATGDWLAPSQPYSVGMPAI"
BASE COUNT          349 a          800 c          942 g          470 t
ORIGIN      
        1 gcgctttcgc gcgcccgatg cctgccatgg cttccgcatg aactacatca acccgctgac
       61 cggcggcccg gccatgccga cgatctccac cgccatgcgc ctgctgccca aaggcttcgt
      121 ctcggcgccc tatcgctcga cagcgggcac ggtgttcagc gtggtggaag gcaccggcgt
      181 tgcccgtgtg ggcgagcagg ccttccccct cgcgcccagg gatatcttcg tcgtgcccag
      241 ctggttcccc gtcaccatcg aggccggcga ggatctcgtc ctcttcacct actcagacca
      301 gatcgtacag gaaaaactcg atttcttccg ggagaagcgt ggtgatggcc ctggtgatag
      361 cctcgccggc tgatcgccac ccggcccgga tcctgaatgg cagaggggag agacgatgga
      421 agagctgcag gcacgtttca gggccaccat ggcgcattat cccgcaggag tggtgatcgt
      481 tacgacgcgg gatgggcatg gccggttgca agggttcacc gccacgtcct tttcgtcgct
      541 gtcgctggcc ccgccctcga ttcttgtgtg cctggcccat gcggcgttct gctacgaagc
      601 ctttgtcacc tcgcgccatt tcgccatcaa tttcgtgggc gcggggcatg atgggctggc
      661 cttgcgcttt gcccggcgcg gcgaggacaa attcgccggc gtgcctgtcc gcctgacgga
      721 acagggcgtg ccgctgctgt gcgaggcggc cgccacgctg gaatgcgcga ccgacagcca
      781 tgtggtgagt ggcgatcatg cgatactggt tggcaatgtg ctggatgcgg ccatcgccga
      841 gggcagcgat gtgctggtgc attatcagcg ccgcttcggc catgtccgtt tcgcgcgggc
      901 tggtggcgat ccggccgatc atggcgcggt gcgctgagat ggccgccgag gcacccgcgg
      961 gccgggcggg cggctggatg cggcgactgg ccatgccgat cggcgggctg atcgcgctgt
     1021 ttggcctgtg catcggcggg gcgggcgcct ggctgctgtt tctgggcggc agctggtttt
     1081 atgccgcgtt cgggccggca tgctgtgttt gcggtgtgct ggtggcccgg cggcggccgg
     1141 ccggggtctg gctgtgggca gtgatcttca tcgccacatg gggctgggcg ctgggcgagg
     1201 tgggggcgga tttctggccg ctggtgccac gggtggcgcc ggtgatggcg atgggcctgg
     1261 cgatcctgcc ggtggcgccg ggcctgtttg gcgggcgggc cgggtgggtg gcctggccgc
     1321 tggcaggcgg catggggctg gctgtcgtgg cggggatcta tggcatgctg ctgccgcatg
     1381 gggcgatcag gccttctgcg caggcggcgg gctgggtgcg cggcctgcct gccgcggcgg
     1441 ggccagagca ggattggcgg cagtacgggc gcaccgccga tggccagcgc tatgcgccgt
     1501 tcgaccagat caacgcgggc aatgtcgcgc ggctgaaacg ggcctggacc tatcgcaccg
     1561 gcgatatcgc gcaagggccg ctgaaggacg agaacacgcc attgcaggtg ggcgatacgc
     1621 tctatgtctg cacgcccaag gaccgggtca tcgcgctgga tggcgatacc gggcagcaga
     1681 aatgggtgtt cgatgccagg gcgacagggc ccgaattcgt gctttgccgc agccttggct
     1741 atgccgatct ggcccgtggc gggcatgggg aggggcggcc cgctgctccg tgcacccagc
     1801 gccttttcct gaccaccacc gatggccggt tgatggcgct ggatgcgctg tccggcaagc
     1861 cctgcgccgc tttcggcaag gcgggaacgg ttgatctgcg cgcggggctg ggcacattcg
     1921 atccgcgctt ctatttttcc aattcggggc cgctggtgat cgagaatgcc cttgtcgtgg
     1981 tgggcgcgcg ggtgaaggac aaccagtccg tggccgagcc atcgggggtg gtgcgcgctt
     2041 tcgatgcaca cagcggcgct ttggtgtggg cctgggatct gggccgcccc gatcgcagcg
     2101 ccgctcctgc gccgggcaag acctacaccc ccggcacgcc gaacatgtgg accacgctga
     2161 catacgaccc cgcgctgggg ctggtttatc tgcccttggg caatgcaacg cccgatttct
     2221 ggggcgggcg ccgccgccag gtggacgagg cctataccga tgcggtggtg gcggtggatt
     2281 atcgcaccgg caaggagcgc tggcactatc gcacgatgaa ccacgacctg tgggattacg
     2341 acctgccggc tccgggcaca ttgtatgaca tgcccgatgg caagggcggc acgctgcccg
     2401 cgctgatcca gatcaccaaa cggggcgaga tcttcgtgct cgatcggcgc aatggccggc
     2461 cgatcacgcc ggtggtgcag cggccggtgc ccaccggccc tgcggccacg ggcgactggc
     2521 tggcgcccag ccagccctat tcggtgggca tgccggcgat c
//
LOCUS       DASRYE010000007         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_176977,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000007 DASRYE010000000
VERSION     DASRYE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176977"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1001)
                     /locus_tag="VFF98_00170"
     CDS             complement(99..1001)
                     /locus_tag="VFF98_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZU62068.1"
                     /translation="MSAWRHGLHSWRWPLALGGALAAALALSLVQVGENEQAVVLRLG
                     DPVRVINGWQPGSAPGTGTAGLAWRLPLLDRVVRVDRRVLWLAVNQQQVLSADQQRLE
                     VDAYARYRIVDPVRMLRTAGTAARLDEQLAPILATLLRQGLATEGFAALLAAGDGPLP
                     DRLRAALDREARGYGVQVIALGIKGARLPDGAPLDDALARMQTSHEEEATAIRAEGEK
                     NAQMIGADAQAQAARIYAASFGKDPQFYDFYRAMKSYETTLAAPPTGSAEAGQQQGTT
                     TFVLTPDTDYLRQFTGAAGAARGR"
     gene            complement(998..2245)
                     /locus_tag="VFF98_00175"
     CDS             complement(998..2245)
                     /locus_tag="VFF98_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZU62069.1"
                     /translation="MRVFEEWIAKWRSGPKAGRPDGANLAMRPGESGDSGDAPPPGEA
                     PLSKPGSPRQGTPRNPWLPDGDPPEAPPSRRPGALPGALEELFFPRSGPSGGSGKPGG
                     PRAPLRLLPPRGNGKSRALSITVSLVALWLLGTSCHQIDSTGQGLVTRGGRYARVLGP
                     GLGFSLPWPFEQVAITDVAAIHTDALPEGDGEQLMLTADRQLVDLGFAVRWNISDLKR
                     YTYELARPDEALRQQAEAAMHAAVGQMPLAEVMAGTGRARLEETVRARLQQELDAAHA
                     GVAVEGVEIRKADPPARVSEAFRKVAAARQEAGADIARAQAFSQQLVARAEGEAGAFD
                     RIYAQYRLAPEVTCRRMYYETMERVLAGSNKIIAPRGTSITLPLADPARQPAANGDQA
                     GNPPPAARPAPQTAGTPTTGTQP"
     gene            2431..3435
                     /locus_tag="VFF98_00180"
     CDS             2431..3435
                     /locus_tag="VFF98_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZU62070.1"
                     /translation="MTPSQPQPDEAALRALLPAALAGRLQSLRLTGQVASLVLEASGL
                     DAAARGALEAEARAALAGAAGVSEVRVALVADRAEPAPAPAGPLIIAVGSGKGGVGKS
                     TLSANLAVALARGGLKVGLVDADIYGPSQARLLSTEGQRPTAHDSKLVPLASPFGVRV
                     LSMGHLIEPGKAIAWRGPMVSGALGQLIDAHWGGAEVLVVDLPPGTGDVQLTMIQKHK
                     PAGAVIVSTPQDLALIDATRAIQLFETAGVPIIGLVENMAGYACPHCGEVSDPFGTGG
                     AEATAREKGVPFLGRVPLDIAIRTASDAGDPPAAGDGPQGAAFAAIARQVAGWLAARR
                     "
     gene            3446..>4671
                     /locus_tag="VFF98_00185"
     CDS             3446..>4671
                     /locus_tag="VFF98_00185"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZU62071.1"
                     /translation="MAPARPPVAPRLPMAFSRRRLLVGAAAGGGLLVAWALSPRRYPV
                     PVPPAAGEAGFGAWLRIGRDGVVTVAVPQLEMGQGITTLLPQVVAMELGADWRMVAAE
                     PQPVSEIFANPPLARRWAQMWLPQAQRLGPPGLWLARRWAEHRHFSATADGLSLAAYE
                     LPARRAAAAARCLLQMAAARQWGVDWQACEAAGGAIRHGASKLGFGALAEAASRLALP
                     RDVPLRAAPAQDPADPAAVAFPRLDLPAKLAGRYPFAGDIRLPGLLYAAIRHAPVGAA
                     ARLAGHEGARAKGIAGFRQLVEGPDWLAAVADNGWAAEQALAAIAPRFVVQAPADSTR
                     AEAALDHGLKAGTAQPVASAGDAAAHLGAHAFSQRYEVAAAFHAPLETATATARLASG
                     PLGLRGAEVWVASQAPG"
BASE COUNT          565 a         1621 c         1801 g          684 t
ORIGIN      
        1 atcgctgcaa ccgccggcag gcggcggctt tgatacatat tacgagcccc gggcctgaac
       61 cgagtttgaa tgatgcgggc cagggctggc aagcgccttc atcggccgcg cgccgcgccg
      121 gcagcgccgg tgaactggcg cagatagtcg gtgtcgggcg tcagcacgaa ggtggttgtg
      181 ccttgctgct ggccggcctc ggcgctgccg gtcggggggg cggcaagggt tgtctcatag
      241 ctcttcatcg cgcggtagaa atcgtagaac tgcggatcct tgccaaagct tgcggcatag
      301 atgcgggcgg cctgggcctg ggcatcggcg ccgatcatct gggcgttctt ctcgccctcg
      361 gcgcggatgg cggtggcttc ttcctcgtgg ctggtctgca tgcgggccag ggcatcgtcc
      421 agcggggcgc catcgggcag gcgggcgccc ttgatgccaa gggcgatcac ctgcacgcca
      481 tagccgcgcg cttcccggtc gagcgctgcg cgcaggcgat cgggcagcgg gccgtcgccg
      541 gcggccagca gggcggcaaa accctcggtg gccagcccct ggcgcagcag ggtggcaagg
      601 atcggcgcca gctgctcgtc cagccgggcg gcggtgccgg ccgtgcgcag catgcgcacc
      661 ggatcgacga tgcgatagcg ggcataggca tccacctcca gccgctgctg atcggccgag
      721 agcacctgct gctggttgac ggccagccac agcacccgcc ggtccacccg taccacgcgg
      781 tcgagcaggg gcaggcgcca ggcgaggccg gcagtgcctg tgccgggggc agagccgggc
      841 tgccagccgt tgatcacccg caccggatcg cccaggcgca gcaccaccgc ctgctcgttc
      901 tcgcccacct gcaccaggct gagcgccagc gctgctgcca gcgcgccgcc cagcgccagc
      961 ggccagcgcc agctgtgcag cccgtgccgc caggcgctca tggctgggtt ccggtagtcg
     1021 gggtgccggc ggtttgcggg gcgggccggg cggcgggcgg cggattgccg gcctgatcgc
     1081 cattggcggc cggctggcgg gccggatcgg ccagcggcag ggtgatgctg gtgccgcgcg
     1141 gggcgatgat cttgttgctg ccggccagca cgcgctccat cgtctcgtaa tacatgcgcc
     1201 ggcaggtcac ttcgggggcc aggcgatatt gcgcatagat cctgtcgaag gcgccggcct
     1261 cgccctcggc ccgggcgacc agctgctgcg agaaggcctg ggcgcgggcg atatcggcgc
     1321 cggcttcctg ccgggctgcg gccactttgc ggaaggcctc gctcacccgg gcgggcggat
     1381 cggccttgcg gatctccacg ccttctaccg ccacgccggc atgggcggca tccagctcct
     1441 gctgcaggcg ggcgcgcacg gtttcttcca gccgggcgcg gccggtgccg gccatcacct
     1501 cggccagcgg catttgcccc acggcggcgt gcatggcggc ttcggcctgc tggcgcaggg
     1561 cctcgtcggg ccgggccagc tcgtaggtgt agcgcttgag atcgctgatg ttccagcgca
     1621 ccgcaaagcc cagatccacc agttggcggt ctgccgtcag catcagctgc tcgccatcgc
     1681 cttcgggcag cgcatcggtg tggatcgcgg cgacatcggt gatcgccacc tgttcgaacg
     1741 gccagggcag gctgaagcca agccccggcc ccagcacacg ggcatagcgc ccgccgcggg
     1801 tgacgaggcc ctgcccggtg ctgtctatct ggtggcagga ggtgcccagc agccacaggg
     1861 ccacaaggct gacggtgatc gacagggccc ggctcttgcc attgccgcgg ggcggcagca
     1921 ggcgcaaggg cgcgcgcggc ccccccggct tgccagagcc gccgctgggc ccgctgcggg
     1981 ggaaaaacag ctcttccagc gcgccgggca gggcgccggg gcggcggctg gggggcgcct
     2041 cgggcgggtc gccatcgggc agccaggggt tgcgcggggt gccctggcgc gggctgcccg
     2101 gcttggacag gggggcctcg cccggcgggg gcgcatcgcc ggaatcgccg ctttcgcccg
     2161 ggcgcatcgc caggttggca ccgtctggcc gccccgcttt agggccgctt cgccacttgg
     2221 cgatccactc ttcgaacaca cgcatggcgc caatgtatag gcggcccttg gcgaatgaac
     2281 agggggcggc ggcggcggcg caagaaaaca ggccatttcc tgtcgccggc cgcgctgcta
     2341 ggggcggcag ggccggaggc gcgccgggct gccgggcgct tgccgatcaa gacaatctgg
     2401 ccgatcagga caatggagta gctgcccgac atgacgcctt cccaacccca accggatgag
     2461 gccgccctgc gcgccctgtt gcctgcagcc cttgccggcc gcctgcaatc gttgcggctg
     2521 acagggcagg tggccagcct ggtgctggag gcatcggggc tggatgcggc cgcgcgcggc
     2581 gcgctggaag cagaggcgcg cgcagcgctg gccggcgctg ccggggtgag cgaagtgcgc
     2641 gtggcgctgg tggccgaccg ggcagagccg gccccggcgc cggccgggcc gctgatcatc
     2701 gcggtgggtt ctggcaaggg gggcgtgggc aaatccaccc tttcggccaa tctggcggtg
     2761 gcgctggcgc gcggcgggct gaaggtgggg ctggtcgatg ccgatatcta tggcccctcg
     2821 caggcgcggc tgctgtccac cgagggccag cgccccaccg cgcatgacag caagctggtg
     2881 ccgctggcca gcccctttgg cgtgcgcgtg ctgtcgatgg gccatctgat cgagccgggc
     2941 aaggcgattg cctggcgcgg gccgatggtg tcgggcgcgc tgggccagct gatcgacgcg
     3001 cattggggcg gggccgaagt gctggtggtg gatctgccgc cgggcacggg cgatgtgcag
     3061 ctgacgatga tccagaagca caagccggcg ggcgcggtga tcgtctccac gccgcaggat
     3121 ctggcgctga tcgatgccac ccgcgcgatc cagctgttcg agacggcggg cgtgccgatc
     3181 atcggcctgg tcgagaacat ggccggctat gcctgcccgc attgcggcga agtgtccgat
     3241 cctttcggca ccggcggcgc cgaggcgacc gcgcgcgaga agggcgtgcc gttccttggc
     3301 cgggtgccgc tggatatcgc catccgcacg gcctccgatg cgggcgatcc gccggccgcc
     3361 ggcgatggcc cgcagggcgc cgcctttgcc gccattgccc ggcaggtggc cggctggctg
     3421 gccgcccggc gctgagctga gcgcgatggc gcccgcccgc ccgcctgtgg cgccccgcct
     3481 gcccatggcc ttcagccggc gccggttgct ggtgggcgcg gcggccggcg gcggcctgct
     3541 ggtggcctgg gcgctcagcc cgcggcgcta tccggtgccg gtgccgccag cggcgggcga
     3601 ggcaggcttt ggcgcctggc tgcgcatcgg gcgcgacggg gtggttaccg tggccgtgcc
     3661 gcagctggag atggggcagg gcatcaccac cctgttgccg caagtggtgg cgatggaact
     3721 gggcgccgat tggcgcatgg tggccgcaga gccgcagccg gtcagcgaga ttttcgccaa
     3781 tccgccgctg gctcgccgct gggcgcagat gtggctgccg caggcgcagc ggctgggccc
     3841 gccgggcctg tggctggcgc gccgctgggc tgaacaccgg catttttccg ccacggccga
     3901 tggcctgtcg ctggccgctt atgagctgcc cgcgcgccgt gccgctgctg ccgcccgctg
     3961 cctgttgcag atggccgcgg cgcgccagtg gggcgtcgat tggcaggcct gcgaggcggc
     4021 ggggggcgcc atccgccatg gtgccagcaa gctgggcttt ggcgcgctgg ccgaagcggc
     4081 atcgcgcttg gccctgccgc gcgatgtgcc gctgcgggca gcgccagcgc aggatccggc
     4141 cgaccctgct gcggtggcct tcccccggct ggatctgccg gcgaaactgg ccgggcgcta
     4201 tccctttgct ggcgatatcc gcctaccggg cctgctgtat gccgccatcc gccatgcgcc
     4261 ggtgggcgcg gcagcgcggc tggccgggca tgagggggcg cgggccaagg gcatcgccgg
     4321 ctttcgccag ctggtggagg gccccgactg gctggccgcg gtggctgaca atggctgggc
     4381 cgccgagcag gcgctggccg ccatcgcccc gcgcttcgtg gtgcaggccc cggccgacag
     4441 cacccgggcc gaagccgcgc tcgatcatgg cctgaaggcg ggcactgccc agccggtggc
     4501 cagtgccggc gatgcggcgg cccatttggg cgcccatgcc ttcagtcagc gctatgaggt
     4561 ggccgccgcc ttccacgccc cgctggaaac cgccacggcc accgcgcggc tggccagcgg
     4621 gccgctgggc ctgcgcggcg ccgaagtgtg ggtggccagc caggcccccg g
//
LOCUS       DASRYE010000008         8711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_194830,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000008 DASRYE010000000
VERSION     DASRYE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194830"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFF98_00190"
     CDS             <1..571
                     /locus_tag="VFF98_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZU62072.1"
                     /translation="LADCAQGAGKIALPREADMIVLSAHKFGGPIGMGALLLRDLSLL
                     RPTGGQERGYRPGTENLPGILAMAAALEAGAGWLAEAARLRARLEAAITAAGGEIIAS
                     AAPRLATIGACRMPGKPAQVQLIRFDALGFAISAGAACSSGSLRASHVLGAMAVPGAN
                     EVIRISIGRETTEAEIDAFAAAWQEIAGR"
     gene            568..1671
                     /locus_tag="VFF98_00195"
     CDS             568..1671
                     /locus_tag="VFF98_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZU62073.1"
                     /translation="MTYLDYQATTPLAPEARAAMRPWLEDGGFANPHSPHRMGRAAAA
                     AVEVARDQVAALMPPGGRVIFTSGATEAINLAILGRARVGALSRIAVSAIEHAAVLET
                     AAASGAVVDVLPVSPAGLVAADAPIAAGTDLVAVMQVNNEIGTIQPVAALAEQAHAAG
                     ALMLVDAVQGAGKIAAPGTGFGGADMIALSAHKIYGPKGIGALWLRDGVALAPILHGG
                     GQEQGLRSGTLSPALCAGFGAAAALCGKLWQSDAEHVDRLWKRARGALPGWVLNGDDG
                     ARWRGNLNLRRAGLDVARLMSDLRDIAFSAGSACASGSGRPSHVLAALGLSAAEAKAS
                     VRIGFGRYTGEEELARALARIDAAARAQGDLAW"
     gene            1665..1997
                     /locus_tag="VFF98_00200"
     CDS             1665..1997
                     /locus_tag="VFF98_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU62074.1"
                     /translation="MVNVTFITTGGDRVAAAGQPGQRLLELAQAAGMPLEGTCEGQMA
                     CSTCHVIVSPDWFARLPAAAADEEDMLDLAAGVAATSRLSCQITLTEAMDGLTVKIPG
                     ESHNMQGA"
     gene            2049..4349
                     /gene="parC"
                     /locus_tag="VFF98_00205"
     CDS             2049..4349
                     /gene="parC"
                     /locus_tag="VFF98_00205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZU62075.1"
                     /translation="MASDTTDLSAEPDPFDAIVDAPFDSALSERYLVYALSTITARSL
                     PDLRDGLKPVHRRLLWAMRQLRLDPAQAFKKSARVVGDVIGKYHPHGDASVYDAMVRL
                     AQNFALRYPLVEGQGNFGNIDGDNAAAYRYTEARLTRTAMQLMAGLEEGTVNFVPTYN
                     GEEEEPEIFPGLFPNLLANGATGIAVGMATSIPSHNVAEIIDATLLLIDNPRAEHADL
                     MEVFRGPDFPTGGVVVDSPAAISTAYAQGKGAIRVRARFSTGRQEDGSWEESGIEKLA
                     GGQWQLVVSEIPYMVAKGKLIEQLAALIADKKLPILEDVRDESDEQIRIVLVPKSRNV
                     DPDLLKESVFKLTELESRFSLNLNVLDASRTPGVLGLKPLLGEWITAQIDILQRRARH
                     RLEKIAQRLELVEGYILAYLNLDRLIEIIRTEDEPRPVMMAEFGLTERQAEAILNMRL
                     RSLRKLEEMELQRERSALLKEQDELTKLLESPTRQRGRLKRDLAALRKEYAEDTALGR
                     RRTSFAEAAPSREFSLEAMIEKEPVTVILSQRGWIRGAKGHLPLDQDWKFKEGDGPAH
                     ALHAQTTDKLLIACDNGRFYTLGADKLPGARGFGEPLRSMLDIDAEAHVVAIAVYRPE
                     AQFLLAASQGRGFAVEAADLLAETRKGRNVMSVKDGVKLAVIREIPPEHDHVAVIGEN
                     RKLVIFPLSELPVLTKGQGVTLQRYRDGSLGDVTTLRLEEGLSWVMGGETGRTRTEKD
                     LLQWKVARGAVGSFPPQGFPRNNRFS"
     gene            complement(4366..5106)
                     /locus_tag="VFF98_00210"
     CDS             complement(4366..5106)
                     /locus_tag="VFF98_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZU62076.1"
                     /translation="MSHAASTDPAQESFSGLLHAGVRAAADMGDFIAAHPLAQLVLLA
                     MLMVLVGGLLRPSMPTFAGLLRFFGNLGLMGALAYGLVLVAGVWLGMDGLPLPSPAST
                     DAPPRQQSVSGGETRIPLASDGHFWVRARVNGVPRRFLVDTGATLTTLSPDTAEAADV
                     RHKGWGEKVMMQTANGTTSADMAVIDELRVGNVVARHLDAVVAPGLGETNVLGMNFLS
                     RLASWRVEHNTLILVAHHPVAAAKKAAD"
     gene            complement(5103..6284)
                     /locus_tag="VFF98_00215"
     CDS             complement(5103..6284)
                     /locus_tag="VFF98_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZU62077.1"
                     /translation="MAEARLPATAWAARADLARLVGVLGADAARYVGGAVRDSLLGLP
                     VKDVDIATPLAPEHVMQLCAQAHIRALPTGIAHGTITAVLDSGPVEITTLRHDVATNG
                     RHAEVAFTEDWQADAARRDFTINALYADPATGEIFDYFGGLADLARRHVRFIGDAEER
                     IREDHLRILRYFRFQARFGALPADGVAESACAALAATLKGLSRERVGMEMMNLLGLAD
                     PAPTIARMAELGVLAVILPEGEPAALAALVAEEQRQAIAPEAIRRLAALLPADAALAE
                     AIASRFRLSGAQKKRLAQAAARPAAGSAQALAYRLGHAAATDLLLLEGRDARALAGWQ
                     APAFPLKGGQIVARGVAAGPEVARLLQQVEARWVAEGFPDEARVEALLAQALAGAAPA
                     P"
     gene            complement(6277..6870)
                     /locus_tag="VFF98_00220"
     CDS             complement(6277..6870)
                     /locus_tag="VFF98_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZU62078.1"
                     /translation="MSALARRLEQLFADGHAGPTPVLERDGRRRHEGPFKPAAVLIAF
                     TDRPDPGLLLIHRPASMRAHPGQVAFPGGRQDEGEPPVEAALREAQEELGIDPALVQV
                     IGATDLYLTGTGYAVTPVVGVVPADIAITPNPHEVASWFEAPADYVLDPARQLRREVE
                     WQGAMQHYTEILWQGHRIWGVTAGIVRNLSRRLNWHG"
     gene            complement(6867..7412)
                     /locus_tag="VFF98_00225"
     CDS             complement(6867..7412)
                     /locus_tag="VFF98_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HZU62079.1"
                     /translation="MPYEPPPSLAALSLGEITALAEGGRLPPVERWAPAHTGDSEMRI
                     ATDGRWFHQGGEITRPAMVRAFSRLLWRDGVGQHWLVTPQEKLAIAVDDAAFIAVDVT
                     AREDALAFRLNTDETVIAGPDHPIRARGSANHPAIYLGLRHGLEARLNRSTWAQLAEI
                     ALAGEGDAVSSQGARFPLVPA"
     gene            complement(7482..8003)
                     /locus_tag="VFF98_00230"
     CDS             complement(7482..8003)
                     /locus_tag="VFF98_00230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZU62080.1"
                     /translation="MAANSTTLIPLDNVDPALVEALLDRAFEPGRHQRTAYKVREGVD
                     WLPGLSFAAIDDEEHLVGTIQCWPVALTTPEGKAHPMIMVGPVAVLPEVQGAGFGKAL
                     MTGCLAALHPQATLPQVMIGDPEYYGRFWGFTADFTGGWRLPGPWEAQRLLVRAANPA
                     ILPAEGMLGPWQG"
     gene            8114..8707
                     /locus_tag="VFF98_00235"
     CDS             8114..8707
                     /locus_tag="VFF98_00235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZU62081.1"
                     /translation="MSIAIIPGVDEAGRGPLAGPVVAGAVALMGADIAGLADSKKLSA
                     KRRAALEGEIKAQALWAVGVVEAEEIDRINIFAATMKAMALAMAGLAAQLPQAPQLVL
                     IDGNKTPEGRTPGWIWPARAIVGGDATEPAISAASIIAKQHRDAIMAELARAHPAYGW
                     QRNAGYGTAEHLAALSAHGPTVHHRRSFGPVAQLRLL"
BASE COUNT         1351 a         2947 c         3124 g         1289 t
ORIGIN      
        1 gctggccgat tgcgcgcaag gggcgggcaa gatcgccctg ccgcgagagg ccgacatgat
       61 cgtgctttcg gcccacaagt ttggcgggcc gatcggcatg ggcgcgctgc tgctgcgcga
      121 tctgtcgctg ctgcgcccca ccggcgggca ggagcgcggc tatcgcccgg gcacggagaa
      181 cctgccgggt atcctggcca tggccgccgc gctggaagcg ggcgccggct ggctggccga
      241 ggcggcccgg ctgcgcgccc ggctggaagc ggcgatcacc gctgccggcg gcgagatcat
      301 cgccagcgcg gccccgcgcc ttgccacgat cggcgcctgc cgcatgccgg gcaagcctgc
      361 gcaggtgcag ctgatccgct tcgatgcgct gggctttgcc atttcggcag gggcggcctg
      421 ttcctcaggc tcgcttcggg ccagccatgt gctgggggcg atggcggtgc ccggcgccaa
      481 tgaggtgatc cgcatctcta tcggccgcga aaccaccgag gccgagatcg acgcctttgc
      541 cgccgcctgg caggagatcg ccggacgatg acctatcttg actatcaggc caccacgccg
      601 ctggcccccg aggcgcgcgc ggccatgcgg ccctggctgg aggatggtgg ctttgccaac
      661 ccccacagcc cccaccgcat gggccgggcg gcggcagcgg cggtggaagt ggcgcgcgat
      721 caggtggccg cgctgatgcc gccgggcggg cgggtgatct tcaccagcgg ggcgaccgag
      781 gcgatcaacc ttgcgatcct gggccgggcg cgcgtggggg cgctctcgcg catcgcggta
      841 tcggccatcg agcatgccgc cgtgctggaa acggcggcgg ccagcggcgc tgtggtggat
      901 gtgctgccgg tatcgcccgc agggctggtg gcggccgatg cccccatcgc ggccggcacc
      961 gatctggtgg cggtgatgca ggtgaacaac gagatcggca cgatccagcc ggtggccgcc
     1021 cttgccgaac aggcccatgc cgccggcgcg ctgatgctgg tggatgcggt gcagggggca
     1081 ggcaagatcg ccgcgcccgg caccggcttt ggcggggccg acatgatcgc cctttctgcc
     1141 cacaagatct atggccccaa gggcattggc gccttgtggc tgcgcgatgg ggtggcgctg
     1201 gcgccgatcc tccatggcgg cgggcaggaa cagggcctgc gttcgggcac gctgtcgccg
     1261 gccttgtgcg cgggcttcgg ggcggcggcg gcgctgtgcg gcaagctgtg gcaaagcgat
     1321 gcggaacatg tggacaggct gtggaaaagg gcgcgcggcg cgctgccggg ctgggtgctc
     1381 aatggcgatg acggggcgcg ctggcggggc aatctcaacc tcaggcgggc cgggctggat
     1441 gtggcgcggc tgatgagcga tctgcgcgac atcgcctttt cggccggcag cgcctgtgcc
     1501 tcgggctctg gccggcccag ccatgtgctg gccgcgctcg gcctctcggc ggcggaggca
     1561 aaagcctctg tgcgcatcgg tttcgggcgc tatacggggg aggaggaact cgcccgggcc
     1621 ttggcgcgaa tcgatgccgc cgcccgcgcg cagggagatt tggcgtggta aatgtgactt
     1681 tcatcaccac cggcggcgat cgtgtggccg ctgccggcca gcccggccag cgcctgctgg
     1741 aactggccca ggccgcgggc atgccgctgg aaggcacctg cgaggggcag atggcctgtt
     1801 ccacctgcca tgtcatcgtc tcgccagact ggttcgcccg cctgcccgcc gccgctgccg
     1861 acgaggagga catgctggac ctggccgccg gcgttgccgc caccagccgc ctgtcgtgcc
     1921 agatcaccct gaccgaggcg atggacgggc tgactgtcaa aattcccggg gaaagccaca
     1981 atatgcaggg cgcctgaggc ggcgcgctct tggcggaagg ggggcgggcc tgctaggcct
     2041 tgccggccat ggccagcgac acgacagacc tttccgccga acccgatcct ttcgacgcga
     2101 tcgtcgatgc gcctttcgac agtgccctgt cggaacgcta tctcgtctat gcgctgtcca
     2161 ccatcaccgc ccgctctctg ccagacctgc gcgacgggct gaagccggtg caccgccggc
     2221 tgctgtgggc gatgcggcag ttgcggctgg atccggcgca ggccttcaag aaatcggccc
     2281 gtgtggtggg cgatgtcatc ggcaaatatc acccgcatgg cgatgcctcg gtctatgacg
     2341 cgatggtgcg cctcgcccag aatttcgcgc tgcgctatcc gctggtggaa gggcagggca
     2401 atttcggcaa tatcgatggc gataatgccg cggcctatcg ctataccgag gcgcggctga
     2461 cgcgcacggc catgcagctg atggccgggc tggaagaagg cacggtcaat ttcgtgccga
     2521 cctataatgg cgaggaggaa gagcccgaga tcttccccgg cctgttcccc aacctgctgg
     2581 ccaatggcgc caccggcatt gccgtgggca tggccaccag cattcccagc cacaatgtcg
     2641 ccgagatcat cgacgcaacg ctgctgctga tcgacaaccc ccgcgccgaa catgccgatc
     2701 tgatggaagt gttccgcggc cccgattttc ccaccggcgg cgtggtggtg gacagccccg
     2761 cggcgatcag caccgcctat gcccagggca agggcgccat ccgcgtgcgc gcccgctttt
     2821 ccaccggccg gcaggaggat ggcagctggg aggagagcgg catcgagaag ctggccggcg
     2881 gccagtggca gctggtcgtc tccgaaattc cctatatggt ggccaagggc aagctgatcg
     2941 agcagctggc cgccctcatc gccgacaaga agctgccgat cctggaggac gtgcgcgacg
     3001 agagcgatga gcagatccgc attgtgctgg tgcccaagag ccgcaatgtc gatcccgatc
     3061 tcctcaagga gagcgtcttc aagctgaccg agctggagag ccgtttcagc ctcaacctca
     3121 acgtgctgga tgccagccgc acgcccggcg tgctggggct gaagccgctg ctgggcgaat
     3181 ggatcaccgc ccagatcgac atcctccagc gccgcgcccg ccaccggctg gaaaagatcg
     3241 cccagcggct ggagctggtg gaaggctata tcctcgctta tctcaacctc gaccggctga
     3301 tcgagatcat ccgcaccgag gatgagccaa ggccggtgat gatggccgaa ttcggcctga
     3361 ccgagcggca ggccgaggcc atcctcaaca tgcggctgcg cagcttgcgc aagctggagg
     3421 aaatggagct gcagcgcgag cgctccgccc tgctgaagga gcaggacgag ctgaccaagc
     3481 tgctggaaag ccccactcgc cagcgcggcc ggctgaagcg cgatctggcc gccctgcgca
     3541 aggaatatgc cgaggataca gcgcttggcc ggcgccgcac cagctttgcc gaagccgccc
     3601 ccagccgcga attcagcctg gaggcgatga tcgagaagga gcctgtcacc gtcatccttt
     3661 cgcagcgcgg ctggatcagg ggcgccaagg gccatctgcc gctcgatcag gactggaagt
     3721 tcaaggaggg cgacgggccg gcccatgccc tccacgcgca gaccaccgac aagctgctga
     3781 tcgcctgcga caatggccgc ttctacacgc tgggggccga caagctgccc ggcgcgcgcg
     3841 gctttggcga acccttgcgc tccatgctcg atatcgatgc cgaggcgcat gtggtggcga
     3901 tcgccgtcta tcggcccgag gcgcagttcc tgctggcggc cagccagggg cgcggctttg
     3961 ccgtggaagc ggccgacctg ctggccgaaa cccgcaaggg ccgcaatgtg atgagcgtga
     4021 aggatggcgt gaagctggcg gtgatccgcg agatcccgcc cgagcacgac catgtggcgg
     4081 tgatcggcga gaaccgcaag ctggtgatct tcccgctgtc cgaactgccg gtgttgacca
     4141 aggggcaggg ggtgacgctg cagcgctatc gcgatggcag cctgggcgat gtgacgacgc
     4201 tgcggctgga agagggcctt tcctgggtga tgggcggcga gaccggccgc acccgcaccg
     4261 agaaggacct gctgcaatgg aaggtggcgc gcggcgctgt gggcagcttc ccgccgcagg
     4321 gcttcccgcg caacaaccgc ttttcctgag gcgcaaggcg gcgcgctagt ccgccgcctt
     4381 tttcgcggcc gccacggggt ggtgggccac caggatcagc gtgttgtgct ccacccgcca
     4441 gctggccagg cgcgagagga agttcatccc cagcacgttg gtttcgccca gccccggggc
     4501 caccaccgca tcaaggtggc gggccactac attgcccacg cgcagctcgt cgatcaccgc
     4561 catgtcggcg ctggtggtgc cgttggcggt ctgcatcatc actttctcgc cccagccctt
     4621 gtggcgcaca tcggcggcct cggcggtgtc gggggaaagg gtggtgaggg tggcgccagt
     4681 gtcgaccaga aagcggcggg gcaccccgtt cacccgcgcg cgcacccaga aatgcccgtc
     4741 gctggccagc ggaatgcggg tttcgccgcc gctcacgctt tgctggcggg ggggcgcatc
     4801 ggtgctggcg gggctgggca ggggcaggcc atccatgccc agccacacgc cggccaccag
     4861 caccagccca taggccagcg cccccatcag cccaagattg ccgaagaagc gcaacagccc
     4921 ggcaaatgtc ggcatggagg ggcgcagcag cccgcccacc agcaccatca gcatggccag
     4981 caacaccagc tgcgccagcg gatgggcggc gatgaaatca cccatgtcgg cagcggccct
     5041 caccccggcg tggagcaggc cggaaaagct ctcctgtgcg ggatcggtgc tggcggcatg
     5101 gctcatgggg cgggggcggc tccggccagg gcctgtgcca gcagcgcctc gacgcgcgcc
     5161 tcatcgggga agccttctgc cacccagcgc gcttccacct gctgcaacag gcgggcgacc
     5221 tctggcccgg cagccacccc gcgcgccacg atctgcccgc ccttcagcgg aaaagctggc
     5281 gcctgccagc cggccagcgc gcgggcatcg cgtccttcca gcagcagcag atcggtggca
     5341 gcggcatggc ccagccgata ggccagcgcc tgcgcagagc ctgccgcagg gcgggcggcg
     5401 gcctgcgcca gccgcttctt ctgcgcgccc gacaggcgaa agcggctggc aatcgcctct
     5461 gccagcgcgg catcggccgg cagcagcgcg gccaggcggc ggatcgcctc gggggcgatg
     5521 gcctggcgct gctcttcggc caccagcgct gccagcgcgg ccggctcgcc ctcgggcagg
     5581 atcaccgcca gcacgcccag ttcggccatg cgggcgatgg ttggcgccgg gtctgccagg
     5641 cccagcaggt tcatcatctc catccccacc cgctcgcgcg agaggccctt cagcgtggcc
     5701 gccagcgccg cgcaggcgct ttcggccacg ccatcggccg gcagggcgcc aaagcgcgcc
     5761 tgaaagcgga aatagcgcag gatgcgcaga tggtcctcgc ggatgcgttc ctcggcatcg
     5821 ccgataaagc gcacatggcg ccgcgccaga tccgccagcc cgccgaaata gtcgaagatc
     5881 tcgccggtgg caggatcagc atagagggcg ttgatggtga aatcgcggcg ggcggcatcg
     5941 gcctgccagt cctcggtgaa ggccacttcg gcatggcggc cgttggtggc cacatcatgg
     6001 cgcagggtgg tgatctccac cggcccgctg tccagcacgg cggtgatggt gccatgggca
     6061 atgccggtgg gcagggcgcg gatatgggcc tgtgcgcaca attgcatcac atgttcgggc
     6121 gccagcggcg tggcgatatc cacatccttc accggcaggc ccagcaggct gtctcgcacc
     6181 gcgccgccga catagcgcgc cgcatcggcg cccagcacgc ccaccagccg ggccagatct
     6241 gcccgggcgg cccaggcggt ggcgggcagg cgggcctcag ccatgccagt tcagtcggcg
     6301 cgacaggttg cgcacgatgc ccgccgtgac gccccagatg cggtggccct gccagagaat
     6361 ttcggtgtaa tgctgcatgg ccccctgcca ttccacttcg cggcgcaatt gccgggccgg
     6421 atcgagcaca taatcggccg gcgcctcgaa ccagctggcc acttcatgcg ggttgggcgt
     6481 gatggcgata tcggccggca ccacccccac caccggcgtg acggcatagc cggtgccggt
     6541 gagatagagg tctgtggcgc cgatcacctg caccagggca ggatcgatgc ccagctcctc
     6601 ctgcgcctcg cgcagggccg cttccaccgg cggctcgccc tcgtcctgcc ggccgccggg
     6661 aaaggccacc tggcccggat gcgcgcgcat gctggccggg cggtggatca gcagcaggcc
     6721 ggggtcgggc cggtcggtaa aggcgatcag cacggcggcg ggcttgaagg ggccttcgtg
     6781 gcgccggcgc ccgtcgcgct ccagcacggg ggtggggccg gcatggccat cggcaaacag
     6841 ctgctccagc cggcgggcca gcgcgctcat gccggcacca gcgggaagcg ggcgccctgg
     6901 ctgctcaccg catcgccctc gccggcaagg gctatctcgg ccagctgggc ccaggtgctg
     6961 cggttcagcc gcgcttccag cccgtggcgc aggccaaggt agatggccgg atgattggcg
     7021 ctgccccgcg cccgaatggg atgatcgggg ccggcgatca ccgtctcgtc ggtgttgagg
     7081 cggaaggcca gcgcgtcttc acgggcggtc acgtccaccg cgatgaaggc ggcatcgtcc
     7141 accgcgatgg ccagcttttc ctgcggggtg acgagccagt gctggcccac cccatcgcgc
     7201 cacagcaggc gcgagaaggc gcgcaccatc gccgggcggg tgatctcgcc gccctggtgg
     7261 aaccagcgcc cgtctgtcgc aatgcgcatc tcgctgtcgc cggtgtgggc aggcgcccag
     7321 cgctccaccg ggggcaggcg gcccccttcg gcaagagccg tgatctcgcc cagcgaaagg
     7381 gcggcaagcg atggcggcgg ttcgtagggc atgggtctgc tatggatctg cctgcaaggc
     7441 gatgccagat cgcgcccgcc gcggcaagac tggccgaagg atcagccctg ccaggggccc
     7501 agcatgcctt ctgccggcag gatggcggga ttggccgcgc gcaccagcag gcgctgggct
     7561 tcccacgggc cgggcaggcg ccagccgccg gtgaaatcgg ccgtgaagcc ccagaagcgg
     7621 ccgtaatatt ccggatcgcc gatcatcacc tggggcagcg tggcctgcgg gtgcagggcg
     7681 gccaggcagc ctgtcatcag cgccttgcca aagcctgcgc cctgcacttc gggcagcacc
     7741 gccaccgggc ccaccatgat catcggatgg gccttgcctt ccggcgtggt cagcgccacc
     7801 ggccagcact ggatggtgcc caccagatgc tcctcatcgt cgatcgcggc gaagctgagg
     7861 ccgggcagcc agtccactcc ttcgcgcacc ttgtaggcgg tgcgctggtg gcggccgggc
     7921 tcgaaggcac gatcgagcag cgcttcgacg agtgcgggat cgacattgtc gagcgggatg
     7981 agcgtggtgc tgttggcggc cataatggcc gcgccgatag gcgagggggg ggacgggcgc
     8041 aacgtttaat cttgcacagg ggcttgccgg cgcgccgcgg gcatgggcaa gaagtgggca
     8101 tgagcatcag gatatgagca tcgccatcat ccccggggtg gacgaggccg ggcgcggccc
     8161 gctggcaggg ccggtggtgg cgggcgctgt ggcgctgatg ggcgccgata tcgccgggct
     8221 ggccgattcg aagaagcttt cggccaagcg ccgtgccgcg ctggagggcg agatcaaggc
     8281 gcaggcgctg tgggcggtgg gcgtggtgga ggccgaggag atcgaccgca tcaacatctt
     8341 tgccgccacc atgaaggcga tggcgctggc gatggcgggc ctggccgcgc aattgccgca
     8401 agcgccccaa ctggtgctga tcgatggcaa caagacgccc gagggccgca cccccggctg
     8461 gatctggccg gcgcgcgcca tcgttggcgg cgatgcgaca gagccggcga tctcggccgc
     8521 cagcatcatc gccaagcagc accgcgatgc gatcatggcc gagctggccc gcgcccaccc
     8581 ggcctatggc tggcagcgca atgctggcta tggcacggcc gagcatctgg cagcgctcag
     8641 cgcccatggg cccacggtgc atcaccggcg cagttttggc ccggtggcgc agcttcgcct
     8701 gctgtagcgg c
//
LOCUS       DASRYE010000009         7093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_106557,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000009 DASRYE010000000
VERSION     DASRYE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106557"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFF98_00240"
     CDS             <1..191
                     /locus_tag="VFF98_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62082.1"
                     /translation="NGGRRLAVELPQLDAQGRVIGALSTSFIVTAGHDAGKAYAEALA
                     VRDEIARQTPSLAALLAP"
     gene            complement(196..1599)
                     /locus_tag="VFF98_00245"
     CDS             complement(196..1599)
                     /locus_tag="VFF98_00245"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZU62083.1"
                     /translation="MKAPLRRVPWSLTGRLWVGIVLPLSVLAVALGLGGALMIRTSVE
                     TVNDRILSAASRAIADSLSIDEGHLELNLSPGIFGMLEDNARDSVFYAVRRDATVLTG
                     YADLPDIVPQGLGDLQMRFGTAHYHGRDIRIVAEGRRMPGIARPLVIEVAETMKTREA
                     ASRHLLLALFALEAMLVMLTTVLLPLALRWGMQPLRRLHAQMEARDGADLTPLPTDNV
                     PGELRYLVEAFNGMLARLDTALQAMRQFTADASHQMRTPLSILRTHIALLRDASLSDT
                     EARASIADIDEASGRLQRLLVQLLALARADNAAAEELALAPIDLGALARDVAAEHVPA
                     AMAAGIDLQFVGDETPARALGHAALAGELLANLIDNAVRYNHPGGQVQLRIEREGDRL
                     LVVIEDNGPGIPAADRERVFERFTRLRRDGTRSGSGLGLPIARMLAGVVGAQLTLVDS
                     QALGGLRACVAFQAIAP"
     gene            complement(1596..2264)
                     /locus_tag="VFF98_00250"
     CDS             complement(1596..2264)
                     /locus_tag="VFF98_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU62084.1"
                     /translation="MARILLVEDDPLLARGLAASLRASGYTVDTAKDGEMGLMLAQDE
                     PYALISLDIGLPDMDGFEVLRVLRRSGVKTPVLMLTARDAINDRVNGLDLGADDYMLK
                     PFEPVELAARIRALLRRAQGEASPVLTVGKLTIDSARVLAMVDGRDLGLRRREWTVLE
                     RLAARVGKVVAKDRLADEIFSFDEPVTPNTIEVYIGRLRRKLEPDGPEIRTIRGIGYM
                     LIDA"
     gene            2517..3461
                     /locus_tag="VFF98_00255"
     CDS             2517..3461
                     /locus_tag="VFF98_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62085.1"
                     /translation="MLVWSAIDAGKAARLIADFEARFPGVRVEYVELSARDIYARFLA
                     AQHSGQPLPDFLWSSAMDLQIKLVNDGYAQSYLSPEIAHVAPWSNWDNQAWGVTAEPI
                     VFVYNRQLISDRDMPTTHSALADYLARRPAPPRATVATYDLPTSAVGYLYLEQDQQAS
                     HAMWPLLKQMADNQLHVFANAEQIMRDVESGRAAIGYDIIGSYALDETRRDARLGMVL
                     PQDYTLVVSRIAMIPRGAPHAAAARLFLDFLLSRKGQADLAEEFMPSIRKDVPTPPAL
                     AAGEAPQRAIRVGPGLLVDSDQMTRDYVVRRWRQSLAD"
     gene            3545..4090
                     /locus_tag="VFF98_00260"
     CDS             3545..4090
                     /locus_tag="VFF98_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZU62086.1"
                     /translation="MLKPALFCTPLLTAALPIAALLAAPADARPLPLPLARAAMVHPD
                     GSPAGRVTLARRYGHLLLVVDLAGLPPGEHGLHFHAVGTCEGPDFKSAGGHLNPEGHQ
                     HGMLNPMGSHLGDLPNFIADAQGHAQLQIALDQLPDKLLPHLFDADGTAIVVHAAADD
                     YRTDPSGNSGARIACGVLRKP"
     gene            4419..5729
                     /locus_tag="VFF98_00265"
     CDS             4419..5729
                     /locus_tag="VFF98_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZU62087.1"
                     /translation="MEKLSYGAGELASNLSWNMAAGFLLIYYTDVALLPVASLGVLML
                     VTRVLDAIFDPLAGLVVDRTRSRKGKARPYLLYGVVPFGLLFVATFSVPQIGMWGKII
                     YAYVTFTLLGLLYAFLYVPYSALLPMLTRRRDEKTQLGSYRAMATSLASILAYGAALP
                     LINLLGGANRQQGYMLTAAVMAVVTGGLYWLTYANCKERHTRPLSAGLPLRTQFARLL
                     SNRVWLLAFIGGLLIFIKIGVMVSSLAFFVKDVMGRPGLVSVILPLMSVMIFIGGAIS
                     RPVVNRFGIRRGTIGALLVSSLLVAGMAFAEGHVWLFVGLYVLANVVGGIQAAALFIL
                     GADAVDWHEAHYGERNEGLTASGISFGLKVGIAIGAAGTAFALGAAGYDPAHASAPAN
                     TALRWLTYGAPLLLNSLLMLCWSRCGGAGQAQGQSSIPAEHAPA"
     gene            5746..6138
                     /locus_tag="VFF98_00270"
     CDS             5746..6138
                     /locus_tag="VFF98_00270"
                     /inference="COORDINATES: protein motif:HMM:NF040396.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6379 domain-containing protein"
                     /protein_id="HZU62088.1"
                     /translation="MMEHRVVSDDGLIVEEGGCVIDVRLPWYRSLPLSVVEIGQVVVD
                     GQLIDPARITFELEGQRYRLDELRPHTDVWWYVLDSAFLHIAGLPLLAGGEHQVSVTI
                     QVRPPYINGLNRIVKTEKRLKAREGVPA"
     gene            6135..>7093
                     /locus_tag="VFF98_00275"
     CDS             6135..>7093
                     /locus_tag="VFF98_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU62089.1"
                     /translation="MSNSQKGLKLGTTLYSMTPAFHGREYGFEELVRKVAAEGLGPGL
                     EVVGFQSIRGFPVVTDEFADRFKALIEETGLTPSCLGINADLRLDPDRPMSDDESLAY
                     HARQLEAAAKLGFPLVRYQYPAGPAVIERLVPLAERIGVRMGLEIHAPHHTQHPDVLA
                     FRDMYERVGSPMLGFIPDFGATVRAVPPSFFEAQRANGVPEDLITLAQDFWSRDGDNY
                     TRRDLYVAEARQRGFDDRLIMMLVIIFGLFSRAEPESWAEIMPQVIHIHGKFFDFDAN
                     GDEIAVDYARILPVFVQGGYDGFMSSEYEGHMWNDADGFEKIR"
BASE COUNT         1144 a         2325 c         2363 g         1261 t
ORIGIN      
        1 ccaatggcgg caggcggctg gcggttgaac tgccccagct ggatgcgcag ggccgcgtga
       61 tcggcgcgct gagcaccagc ttcatcgtga ccgccggaca tgacgccggc aaggcctatg
      121 ccgaggcgct ggccgtgcgc gacgagatcg cccgccagac cccttcgctg gccgccctgc
      181 tggcgccctg aaggcctagg gggcgatcgc ctggaaggcc acgcaggcgc gcaatccgcc
      241 cagcgcctgg ctgtcgacca gcgtcagctg cgcgcccacc acgccggcca gcatccgcgc
      301 gatgggcagg cccagcccgc tgcccgagcg cgtgccatca cgccgcaggc gggtgaagcg
      361 ttcgaacacc cgctcgcggt ctgctgcggg aatgcccggc ccattgtcct cgatgacgac
      421 gagcaggcga tcgccctcgc gctcgatgcg cagctggacc tggccgccgg ggtggttgta
      481 gcgcacggca ttgtcgatga gattggccag cagctcgccg gccagcgcgg catggccaag
      541 cgcccgggcg ggcgtttcat cgccaacgaa ttgaaggtcg atgccggcgg ccatggcggc
      601 cggcacatgt tcggccgcca catcgcgggc cagcgcgcca aggtcgatgg gggccagcgc
      661 cagttcctcg gcggcggcat tgtcggcgcg ggccagcgcc agcaattgca cgagcaggcg
      721 ctgcagccgg ccgctggcct cgtcgatatc ggcgatcgag gcgcgcgctt ctgtgtcgga
      781 caggctggca tcgcgcagca gggcgatatg ggtgcgcagg atcgagagcg gcgtgcgcat
      841 ctggtgcgag gcatcggcgg tgaactggcg catcgcctgc aaggcggtgt cgagccgggc
      901 cagcatgccg ttgaacgcct ccaccagata gcgcagctcg cccggcacat tgtctgtcgg
      961 caggggggtg aggtcggcgc cgtcgcgcgc ttccatctgg gcgtgcaggc gccgcaaggg
     1021 ctgcatcccc cagcgcagcg ccagcggcag cagcaccgtc gtcagcatca ccagcatcgc
     1081 ctcgagcgcg aacagcgcca gcagcaggtg gcgcgatgcc gcctcgcggg ttttcatggt
     1141 ttcggccacc tcgattacca gcgggcgggc aatgccgggc atccgccgcc cttcggccac
     1201 gatgcggata tcgcgcccgt gatagtgggc ggtgccgaag cgcatctgca gatcgcccag
     1261 cccctgcggc acgatatcgg gcaggtcggc atagccggtc agcaccgtgg catcgcggcg
     1321 cacggcataa aagacactgt cgcgcgcgtt atcctccagc atgccgaaga tgccgggcga
     1381 gaggttgagt tccagatgcc cctcgtcgat cgacagggaa tcggcgatcg cgcgtgaggc
     1441 ggccgagagg atgcggtcgt tcacggtttc caccgaggtt ctgatcatca gcgcgccgcc
     1501 caggcccagc gccaccgcca gcaccgacag cggcagcacg atgccaaccc acaagcgccc
     1561 cgtcaacgac cagggaacac gccgcagcgg cgctttcatg cgtctatgag catatagccg
     1621 atgccgcgga tggtgcggat ttccggcccg tcgggttcca gcttgcggcg caggcggccg
     1681 atatagacct cgatggtgtt gggcgttacc ggctcgtcga agctgaagat ctcgtcggcc
     1741 agccggtcct tggcaacgac cttgcccacg cgcgccgcca gccgctccag cactgtccat
     1801 tcgcgccggc gcaggcccag atcgcgccca tcgaccatcg ccagcacccg ggcggaatcg
     1861 atggtcagct tgcccactgt cagcaccggc gatgcctcgc cctgggcccg gcgcagcagc
     1921 gcgcggatgc gcgcagccag ttcgacaggc tcgaaaggct tgagcatata gtcgtcggcg
     1981 cccagatcca gcccgttcac ccggtcgttg atggcatcgc gcgcggtcag catcagcacc
     2041 ggcgttttca ccccgctgcg gcgcaacacc cgcagcacct cgaagccgtc catgtctggc
     2101 aggccgatat ccagcgaaat cagcgcatag ggctcatcct gagccagcat cagccccatt
     2161 tcgccatcct tggcggtatc gaccgtatag cccgaggcgc gcagcgaagc ggccaatcca
     2221 cgcgccagca acggatcgtc ctcgaccaga agaattcttg ccaccctcgc ccacctttta
     2281 ttgacggtgc catcatagac gaggaaactg cccgcgcaaa cggaaccgtc aggcgttcat
     2341 ggcaggaagg gaaggggtgg gccatgcaaa ggccagggcg gccgtggcgc cgcgcagctt
     2401 gttggccgat actggcgctg ctgggcgctt gtcagccagc gccggaagat ggactgcccg
     2461 cttattaccc ccgcgattat gtcgaacttg tcggtttggc caaggccggc aagcgcgtgc
     2521 tggtctggtc ggcgatcgat gccggcaagg ccgccaggct catcgccgat ttcgaggcgc
     2581 gctttcccgg cgtgcgggtg gaatatgtcg agctttccgc gcgcgatatc tatgcccggt
     2641 ttctcgccgc gcagcacagc ggccagccgc tgcccgattt cctgtggtcc tcggcgatgg
     2701 atctgcagat caagctggtc aacgacggct atgcgcagag ctatctctcg cccgagatcg
     2761 cccatgtcgc cccctggtcg aactgggaca accaggcctg gggggtaacg gcagagccga
     2821 tcgtctttgt ctataatcgc cagctgatca gcgatcggga catgcccacc acccattcgg
     2881 ccctggccga ttatctggcc cggcggcccg ccccgccgcg cgccaccgtt gccacatatg
     2941 atctgcccac ctcggcggtc ggctatctct atctcgagca ggatcagcag gcctcgcacg
     3001 ccatgtggcc gctgctgaag cagatggccg ataatcagct gcatgtcttt gccaatgcgg
     3061 aacagatcat gcgcgatgtg gaaagcgggc gggcggcgat tggctatgat atcatcggct
     3121 cctacgcgct ggacgagaca aggcgcgatg cccggctggg catggtgctg ccgcaggatt
     3181 acacgctggt cgtctcgcgc attgcgatga tcccgcgcgg cgcgccccat gcggcggcgg
     3241 cgcggctgtt tctcgatttc ctgctgtcac gcaaggggca ggcggatctg gccgaagagt
     3301 tcatgccatc gatccgcaag gatgtgccca cgccccccgc gctggccgcg ggcgaggcgc
     3361 cccagcgcgc gatcagggtg ggcccgggcc tgctggtgga cagcgaccag atgacgaggg
     3421 attatgtcgt gcggcgctgg cgccagtctc tggcggatta ggcggggtgg gcggcgatct
     3481 tgccatgcgg ggcgcgctgc cctatccttg ccggcgccct ttccttgctg gcagggagaa
     3541 cgccatgctc aagcccgccc tcttctgcac cccgctgctc accgctgccc tgcccatcgc
     3601 tgccctgctt gccgcccccg ctgatgcgcg gcccttgccg ctgccgctgg caagggcggc
     3661 catggtgcat cccgatggct cgccggcggg cagggtgacg ttggccaggc gctatggcca
     3721 tctgctgctg gtggtggacc tggccggcct gccgccgggc gagcatggcc tgcatttcca
     3781 cgccgtgggc acctgtgagg gccccgattt caaaagcgcc ggcggccacc tcaaccccga
     3841 ggggcaccag cacggcatgc tgaacccgat gggcagccat ctgggcgatc tgcccaattt
     3901 catcgccgat gcgcaagggc acgcgcaatt gcagatcgcc ctcgaccagc ttcccgacaa
     3961 gctgctgccg catctgttcg atgccgatgg cacggcgatc gtcgtccacg ccgccgccga
     4021 tgattaccgc accgacccca gcggcaacag cggcgcccgc atcgcctgcg gcgtgctgcg
     4081 caaaccctga cgagcaggcc cgcacccgga aaattcagcc ggaaaattca gccgtgccct
     4141 tgcgggcgcg tgatcgggcc tgtgcccttg cgaaggggcg gaatcagccc tcatcatcac
     4201 gcaaagtgat gattgatata tcgtgcgctg aattgaggcg cggctgtggc ttctttacag
     4261 aaagcctgga ggcgccaagc agcgccgatc gataaaaatt gaaaaaacaa ggccctgact
     4321 ggatcgcgcg gtcggcggga taatatggga gtggattgct ggtgacctca cgcagctctg
     4381 gatcgacgct ggctaccacg gacccgggtt tgagccaggt ggaaaagctc agctatggtg
     4441 ctggcgagct ggcttccaat ctgtcctgga acatggcggc aggctttctg ctgatctact
     4501 ataccgatgt ggccctgctg ccggtggcaa gcctgggtgt gctgatgctt gtcacccgtg
     4561 tgcttgatgc catcttcgat ccgctggccg ggctggtggt ggatcgcacc cgttcccgca
     4621 agggcaaggc gcggccctat ctgctgtatg gcgtggtgcc ctttggcctg ctgttcgttg
     4681 ccactttcag cgtgccgcag atcgggatgt ggggcaagat catctatgcc tatgtcacct
     4741 tcacgctgct cgggctgctc tatgcctttc tctatgtgcc ctacagcgcc ctgctgccca
     4801 tgctcacccg gcgccgcgac gagaagacgc aattgggcag ctatcgggcc atggcaacct
     4861 cgctggcatc gattttggcc tatggcgcgg cgctgccgct catcaacctg ctgggcgggg
     4921 cgaaccggca gcagggctat atgctcacgg ccgcggtcat ggcggtggtc accggcgggc
     4981 tgtactggct gacctatgcc aattgcaagg agcgccacac ccgcccgctg agcgccggcc
     5041 tgccgctgcg cacgcagttt gcgcggctgc tgtcgaaccg ggtctggctg ctggccttca
     5101 tcggcgggtt gctcatcttc atcaagatcg gcgtcatggt ctccagcctt gccttcttcg
     5161 tgaaggatgt gatgggcagg cccggtctcg tctcggtcat cctgccgctc atgtcggtga
     5221 tgattttcat cggcggggcg atctcgcggc cggtggtgaa ccgcttcggc atccgtcggg
     5281 gcacgatcgg ggcgctgctg gtgtccagcc tgctggtggc gggcatggcg tttgccgagg
     5341 gccatgtctg gctgtttgtc ggcctgtatg tgctggccaa tgtggttggc ggcattcagg
     5401 cggcggcgct gttcatcctg ggggccgatg cggttgactg gcacgaggcc cattacggcg
     5461 agcgcaacga ggggctgacg gcctcgggca tcagctttgg cctcaaggtg gggatcgcca
     5521 tcggcgcggc gggcacggcc tttgccctgg gcgctgccgg ctatgacccg gcccatgcct
     5581 cggcccctgc caacaccgcc ctgcgctggc tgacctatgg cgccccgctg ctgctcaaca
     5641 gcctgctgat gctgtgctgg tcgcgctgcg ggggcgcggg gcaggcgcag gggcaatcat
     5701 caatcccggc cgaacacgcg ccggcatgag aggagctttc gagccatgat ggagcatcgg
     5761 gttgtaagtg acgatgggct catcgtcgag gagggcgggt gtgtgatcga tgtcaggctg
     5821 ccctggtatc gctcgctgcc gctgtcggtg gtggagatcg ggcaggtggt ggtggatggc
     5881 cagctcatcg atccggccag gatcaccttc gaactggaag ggcagcgcta tcgcctggac
     5941 gagctgcgcc cccataccga cgtgtggtgg tatgtgctcg acagcgcgtt cctgcatatc
     6001 gccggcctgc cgctgctggc ggggggcgag caccaggtgt cggtcaccat ccaggtccgg
     6061 ccgccctata tcaacgggct gaaccgcatc gtgaaaaccg aaaaacgcct gaaggcacgc
     6121 gaaggagttc cggcatgagc aattcccaga aggggctgaa gctgggcacg accctctaca
     6181 gcatgacccc ggccttccat ggccgcgaat atggcttcga ggagctggtt cgcaaggtgg
     6241 ccgccgaagg gctggggccc ggcctcgaag tggtcggatt ccagagcatt cgcggctttc
     6301 ccgtcgtgac cgatgaattc gccgatcgct tcaaggcgct gatcgaggaa accgggctga
     6361 cgccttcctg ccttggcatc aatgccgatc tgcggctcga tccggaccgg ccgatgagcg
     6421 atgacgaaag cctggcctat cacgcccgcc agctggaagc ggccgccaaa ctgggctttc
     6481 cgcttgtgcg ctatcaatat cccgcagggc cggcggtgat cgagcggctg gtgccgctgg
     6541 ccgagcggat cggcgtcagg atggggctgg aaatccacgc gccgcaccac acgcagcatc
     6601 ccgatgtgct ggccttccgg gacatgtacg agcgcgtcgg ctcgccgatg ctgggcttca
     6661 tccccgattt tggcgccacc gtgcgcgcgg tgccgcccag cttcttcgag gcccagcgcg
     6721 ccaatggcgt gcccgaggat ctcatcacgc tggcgcagga tttctggtcg cgcgatggcg
     6781 acaattacac ccgccgcgac ctctatgtcg ccgaggcccg ccagcgcggc tttgacgacc
     6841 ggctgatcat gatgctggtc atcatcttcg gcctgttcag ccgggcagag ccggaatcct
     6901 gggccgagat catgccccag gtcatccaca tccacggcaa gttcttcgat ttcgatgcca
     6961 atggcgatga aatcgcggtg gattatgcgc gcatcctgcc ggtcttcgtg cagggcgggt
     7021 atgatggctt catgtccagc gaatatgaag ggcacatgtg gaacgatgcc gatggcttcg
     7081 agaagatccg caa
//
LOCUS       DASRYE010000010         9362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_528,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000010 DASRYE010000000
VERSION     DASRYE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFF98_00280"
     CDS             complement(<1..1112)
                     /locus_tag="VFF98_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZU62090.1"
                     /translation="MLLLLLAYLGGALTILSPCILPVLPFVFTRAGRPFLTNGLPMLA
                     GMAAMFALVASLAALGGSWAVAANHYGRWAALGLCALFGLALLVPALAERMSRPLVAL
                     GGRLSQRAGQGGLGPSLLLGMATGLLWAPCAGPILGLVLTGAALHGANAGTGLLLLAY
                     AAGAATSLALVLAVGGRVLRAMQASLGLGEWLRRALGAAVLLAVAAMALGVDTGLLTR
                     ASVAGTNRVEQGLIARFGLAAPPPPPATATPLAALPGAVAWINSPPLGAQALKGHVVL
                     VDFWTYSCINCLRTLPYMRAWAEKYRAAGLVVIGVHAPEFAFEKDLGNVRAAVRRLGI
                     GFPVAVDNNYALWQAFGNQYWPADYLIDARGQLRAQ"
     gene            complement(1160..1828)
                     /gene="msrA"
                     /locus_tag="VFF98_00285"
     CDS             complement(1160..1828)
                     /gene="msrA"
                     /locus_tag="VFF98_00285"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZU62091.1"
                     /translation="MRGLVALLALLLAAPAMAGPVPVPMPAPVPPAAFPPPPPEAPQP
                     HGRVVAVLAGGCFWGMEGVFEHVKGVREVVAGYAGGTALTAHYDIVSSELTNHAEAVR
                     ITYDPAVVSYGDLLRVYFAVAHDPTDADGQYPDRGRSYRSAVFAQNPAQQALAAAYIA
                     ALARAHVFARPIVTTIEAGPFFPAEAEHQHFMRRHPDHPYIRAWDVARVQRLRQRLPA
                     FYQP"
     gene            complement(1825..2313)
                     /gene="msrB"
                     /locus_tag="VFF98_00290"
     CDS             complement(1825..2313)
                     /gene="msrB"
                     /locus_tag="VFF98_00290"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZU62092.1"
                     /translation="MERRAMLGASLSALWLCVPALARAGGGDSADSAWRLSDAAWRQR
                     LAPAAYAILRQGGTEPPWTSPLLHEHRRGRFACAGCDLALFASDTKFDSGTGWPSFWQ
                     ALPGAVARLADRSYGMERTEVRCHRCAGHLGHLFDDGPRPTGLRYCMNGAALQFHPEA
                     RR"
     gene            2449..4239
                     /gene="recQ"
                     /locus_tag="VFF98_00295"
     CDS             2449..4239
                     /gene="recQ"
                     /locus_tag="VFF98_00295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HZU62093.1"
                     /translation="MHESARALHEALHATFGFSGFRGVQEQVVARVLAGRSTLAVMPT
                     GAGKSLTYQLPAVMLAGTCVVVSPLIALMHDQLRSAQANGIRAASLTSADTNRAHTLE
                     RLLAGELDLLYVAPERASQPHFRDLLAEAQISLFAIDEAHCVSEWGHDFRPDYRLLRG
                     LLDAFPDVPRLALTATADGHTRADILAQLGIAPEGLIVAGFDRPNIRYTITARDNPAQ
                     QLKRLIAETPGPGIVYAQTRAQVERLADTLASTGRPVLPYHAGLATEQRAANQAAFVA
                     SEEMVMVATIAFGMGIDKPDVRFVAHAGLPKSLEAYYQETGRAGRDGDPAVALMLWGA
                     EDFARARQRLGELEEHRIPGERARLDALATLVETAGCRRALLRRHFGEDAPAHCGNCD
                     NCLNPPQVSEMTELARKLLSAVFRTGQSFGLGHLEKVLTGVDDERVRARGHDALSVFG
                     IVGKDEAALLRPLARALQARGSLVATEHGGLALAGDARAILKGEAAVAIALPPRAERK
                     PRRPREAGAAMDNPLDNALFEALRQWRRETASAQGVPPYVIFHDSTLREIAAHRPSSL
                     AELGTLQGVGARKLEAYGAALLQLLAAPEA"
     gene            complement(4455..5984)
                     /locus_tag="VFF98_00300"
     CDS             complement(4455..5984)
                     /locus_tag="VFF98_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZU62094.1"
                     /translation="MDMSAPAPASEGAPASEGPGASDGPGAADLFGMARIGAMYVALA
                     IDSIREVVPYPPALAAIPATMPQLLGAIDLRGVVVPVLDLAALLGAAGPEAGPEAQRP
                     GIIMVVRLRDPGRGDRVVGVGLHEICGVIRLGPESLTRMEVAPGIEAPGGGLVGAGFV
                     SNGRSGVLLDMAGLAALPGIAMAEDRLFSAMAKAQRGEPVLCVAAGDLSLGLPARVIE
                     ATVPCRPVAPSPVEDGLWQARVEYNGVQVPVVDTLRLLGLGRSPPARAIATVLLRMGE
                     GRRVALRIDAVRDMVRVRAGQAAGMQGLAFGRAGLIGGLLPAAPASPEGAAAVPMLLL
                     DGDALVAHGELVRLSHLEERPPAAGVLAPAGGPAVSAGADPAARQPYLICRLGDGHHA
                     VPLAQVTEILGRAACALIDLAEAEGPFRAILSHRGLAIPVLDPAAHLGLGPCGDPAFI
                     LLVSNGERVAGFALQGLCAVERLQARGGRQEGRGLGQVVLTGEGRTCSVLDLAALVPG
                     D"
     gene            complement(5992..8757)
                     /locus_tag="VFF98_00305"
     CDS             complement(5992..8757)
                     /locus_tag="VFF98_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HZU62095.1"
                     /translation="MTMQPLGLDLVGKTAALLADAAIILAFDAQSGLAVAGNEEALAG
                     LGGGDLATCAFAGLFADAPALWERLLAGERLVLSGGLPRADGTFLGLHGMAMRAEDRQ
                     GLIHFLGVRSCQGAGDVALLTHRFEAINHALAICQYSPDGILLAANDHFCASVGMAGA
                     DLVGQPFTALWHGADCPADPAAYWQRFAQGRHDLLVRKHRNAAGQEVWLREVFVPTMD
                     GDRLVSVLSYAINITEEQNARADSASRLLAIDRAFALIEFDLEGRILDANANFLRLMG
                     YTLDELKGAHHRIFCDKDYVTSPAYRQFWKKLGSGEFDQGEYKRIRKDQREVWIQASY
                     NPILDADGHPYKIMKVAMDVTQQRLAAIEADSKLAAIDRSQAVVEFDLAGNVLSANAN
                     FLAAMGYAAEDVLGRHHAMFCDPGFVASEEYAAFWQKLARGEFASGIFRRKGRGGRDV
                     WIRATYNPVFDLDGHVAKIVKFAHDITQSHELAVEQAEKIAAIDRSHAMIEFDLAGNI
                     ITANAQFLAVTGYRLDEIQGRHHRLFCDPDMAASADYANFWERLARGEHVAGEFCRRR
                     KGGEDFWIQASYNPINGADGKPVKVCKIASDITAAKLAASEYQARVAAIGRSLAVIEF
                     DLDGNILSANEHFLSTMGYSLREVVGQHHAIFCSPDYVRSRAYADFWLKLNRGEYHAG
                     RFHRVGKYDRDVWIQATYNPILDLQGKVARIVKYAFDITDQVLLEREIEARAGDLGGL
                     VERLSSSISAISGATIAADGLSRSTHADAEKGHDALGKAIEAIELIQTSAAGIAEIVG
                     IIGEIAGQTNLLAFNAEIEAARAGEHGVGFSVVAGEVRKLAERSSTAARDISRLIDQS
                     IQRINQGTDRSREASQAFTGIVDAVHKTGSAIEDISRSAATQDQVSAEVVVLIQRLAA
                     TASRRPD"
     gene            9054..>9362
                     /locus_tag="VFF98_00310"
     CDS             9054..>9362
                     /locus_tag="VFF98_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62096.1"
                     /translation="MTPHRMGPTTTAVPDGNRAALATAHAALRQGCHFLSARSEGDGS
                     GGHRHARLRWRVAGNHLKELDRFLAVLLAEYAQATGHARPPLTPPIPDSLAPAPDSAE
                     A"
BASE COUNT         1544 a         3453 c         3062 g         1303 t
ORIGIN      
        1 tgctgcgcgc gcagctggcc gcgggcatcg atcagatagt ccgccggcca atactggttg
       61 ccgaaggcct gccacagggc ataattatta tccaccgcca ccggaaagcc gatgcccagc
      121 cgccgcaccg ccgcgcgcac attgcccagg tctttctcga aggcgaattc gggcgcgtgc
      181 acgccgatca ccaccagccc ggcggcgcgg tatttctcgg cccaggcgcg catatagggc
      241 agcgtgcgca ggcagttgat gcagctgtag gtccagaaat cgaccagcac gacatggccc
      301 ttcagcgcct gtgcgccaag cggcgggctg ttgatccagg ccactgcccc cggcagcgca
      361 gccagcggcg tggcggtggc cggcggcggc ggcggggcgg ccaggccaaa gcgggcgatc
      421 agcccctgct ccacccgatt ggtgcccgcc accgacgcgc gggtgagcag ccctgtatcc
      481 acccccagcg ccattgccgc caccgccagc agcaccgcag cccccagcgc ccgccgcagc
      541 cattcgccca gccccagcga ggcctgcatg gcgcgcagca cccgcccgcc caccgccagc
      601 accagcgcca gcgaagtggc cgcccctgct gcataggcca gcagcagcag gccggtgccg
      661 gcattggcgc catgcagggc cgcgccggtc aacaccaggc ccaggatcgg cccggcacac
      721 ggcgcccaca acaggcccgt ggccatgccc agcagcagcg aagggcccag ccccccttgc
      781 ccggccctct gcgacaggcg cccgcccagc gccaccagcg gccggctcat ccgctcggcc
      841 agcgccggca ccagcagcgc caggccaaac agcgcgcaca ggcccagcgc cgcccagcgc
      901 ccgtaatggt tggccgccac cgcccagctg ccgcccagcg ccgcaaggct ggccaccagc
      961 gcgaacatcg ccgccatgcc cgccagcatc ggcaagccat tggtgaggaa gggccgcccg
     1021 gcgcgagtga agacaaaggg cagcaccggc aggatgcagg ggctgaggat cgtcagcgcg
     1081 ccgccgagat aggccagaag cagaagcagc atgaaggggc aagcctgtgc agaagggggg
     1141 gaaggaaagc gggcggggcc taaggctgat agaaggcggg caggcgctgg cgcaggcgct
     1201 gcacccgggc cacatcccag gcgcggatat aggggtgatc ggggtggcgg cgcatgaagt
     1261 gctgatgctc cgcctcggcc gggaagaagg gcccggcctc gatggtggta acgatcggcc
     1321 gggcaaacac atgggcgcgg gcaagcgcgg cgatataggc agcggccagc gcctgctgcg
     1381 ccgggttctg cgcgaagacc gccgagcgat agctgcgccc gcggtcggga tactggccat
     1441 cggcatcggt cggatcatgg gccacggcga aatagacccg cagcagatcg ccataggaca
     1501 ccaccgccgg atcataggtg atgcgcaccg cctcggcatg gttggtcagc tctgaagaga
     1561 cgatgtcgta atgggctgtc agcgcggtgc cgccggcata gcctgccacc acctcgcgca
     1621 cgcccttcac atgctcgaac accccctcca tcccccagaa gcagccgccc gccagcaccg
     1681 ccaccacccg gccatggggc tggggcgctt ctggcggcgg cggcggaaag gcggccggcg
     1741 gcactggcgc gggcatgggc actggcaccg gaccggccat ggcgggcgcg gccagcagga
     1801 gggcaaggag ggccaccagc cccctcatcg ccgcgcctcc gggtggaact gcagggccgc
     1861 gccgttcatg caatagcgca ggcccgtggg gcgcggccca tcatcgaaca gatggcccag
     1921 atggccggcg cagcggtggc agcgcacctc tgtgcgctcc atgccgtagg agcgatccgc
     1981 cagccttgcc accgcgcccg gcagcgcctg ccagaagctg ggccagccgg tgccgctgtc
     2041 gaacttggtg tcagaggcga acagggccag gtcgcagccc gcgcaggcaa agcggccgcg
     2101 ccggtgctcg tgcagcagcg ggctggtcca tggcggctcg gtgcccccct ggcgcaggat
     2161 ggcataggcg gcaggggcca ggcgctggcg ccaggcggca tcgctgaggc gccaggcgct
     2221 atcggcactg tcgccgcccc cggcccgcgc aagggccggc acgcacagcc acagcgccga
     2281 caggcttgct cccaacatgg cacggcgttc catcggcagg gcgcctttcg cggttatccg
     2341 gcgccaagcc tagcaccggg cccggcccgc gcaaaggggc cggttctatg ccgcgccttt
     2401 ctgtgttagc cggccgggat ggatatcgct cccccttccg cgccccccat gcacgagtcc
     2461 gcgcgtgccc tgcacgaggc gctgcacgcc actttcggct tttccggctt tcgcggcgtg
     2521 caggaacagg tggtggcccg cgtgctggcg ggccgctcca ccctggcggt gatgcccacg
     2581 ggcgcgggca aatcgctcac ctatcagctg ccggcggtga tgctggcggg cacctgcgtt
     2641 gtcgtcagcc cgctgatcgc gctgatgcac gaccaattgc gcagcgccca ggccaatggc
     2701 atccgcgccg ccagcctgac cagcgccgac accaaccgcg cccacacgct ggagcgcctg
     2761 ctggccggcg agctggacct cctctatgtc gcccccgagc gcgccagcca gccgcatttc
     2821 cgcgatctgc tggcagaggc gcagatcagc ctctttgcca tcgacgaggc ccattgcgtt
     2881 tccgaatggg gccatgattt ccggccggat tatcgcctgc tgcgcggcct gctcgatgcc
     2941 ttccccgatg tgccgcgcct ggcgctgacc gccaccgccg atggccacac ccgggccgat
     3001 atcctcgccc agctgggcat tgcgccagag gggctcatcg tcgccggctt cgaccggccc
     3061 aacatccgct acacaatcac cgcgcgtgac aatcccgccc agcagctgaa gcggctgatc
     3121 gccgagaccc cgggccccgg catcgtctac gcccagaccc gcgcgcaggt ggagcgcctg
     3181 gccgacacgc tggccagcac cggccgcccc gtgctgccct atcacgccgg ccttgccaca
     3241 gagcagcgcg ccgccaacca ggccgctttc gtggccagcg aggagatggt gatggtggcc
     3301 accatcgcct ttggcatggg catcgacaaa cccgatgtgc gctttgtcgc ccatgccggc
     3361 ctgcccaaat cgctggaggc ctattaccag gaaaccggcc gcgccgggcg cgatggcgat
     3421 ccggccgtgg cgctgatgct gtggggagcg gaggatttcg cccgcgcccg ccagcgcctg
     3481 ggcgagctgg aagagcaccg catccccggc gagcgggcac ggctggatgc gctggccacg
     3541 ctggtggaaa ccgccggctg ccgccgcgcg ctgctgcgcc gccatttcgg cgaggacgcc
     3601 cccgcccatt gcggcaattg cgacaattgc ctcaacccgc cgcaagtgag cgagatgacc
     3661 gaactggcgc gcaagctgct ttcagccgtg ttccgcaccg ggcagagctt tggccttggc
     3721 catctcgaaa aggtgctgac cggggtggac gacgaacgcg tgcgcgcccg cgggcatgat
     3781 gcgctcagcg ttttcggcat tgtcggcaag gacgaggccg cgctgctgcg cccgctggcc
     3841 cgcgccctgc aggcccgcgg cagcctggtg gccaccgaac atggcgggct ggcgctggcg
     3901 ggcgatgcgc gggcgatcct gaagggcgag gcggccgtgg ccatcgccct gccgccccgc
     3961 gccgaacgca agccccgccg cccgcgcgag gcgggcgcgg cgatggacaa tccgctcgac
     4021 aatgcgctgt tcgaggccct gcgccaatgg cggcgcgaga cggccagcgc gcaaggggtg
     4081 ccgccctatg tcatcttcca tgattccacc ctgcgcgaga tcgccgcgca ccggccgtct
     4141 tcgctggcgg aactgggcac gctgcagggc gtgggcgcgc gcaagctgga agcttatggc
     4201 gccgcgctgc tgcaattgct ggcagcgcct gaggcctgag acctgcggcg aaaaaaggcg
     4261 cgccgtcttg ccgggagggc ctggaggcaa gccggcgcgc cccctcctcg cggcgctttt
     4321 tcccgaggcg atcggtggaa aaaacggtgt gcggaaggtg aacattcttc tgcacaggga
     4381 aggtacacac aggctttagg gctttggtta agcctgatgc aggatattca cctatccggc
     4441 cggggccccg cccctcaatc gcccggcacc agcgcggcca gatccagcac ggaacaggtg
     4501 cgcccctcgc cggtcaacac cacctggccc aggccccgcc cctcctgccg gcccccgcgc
     4561 gcctgcagcc gttccaccgc gcacaggccc tgcagggcaa agccggccac ccgctcgcca
     4621 ttgctgacca gcaggatgaa ggccggatcg ccgcaagggc ccagccccag atgagcggcc
     4681 ggatcgagca ccggaatggc cagcccccga tgcgacagga tcgcccggaa aggcccttcg
     4741 gcctcggcca gatcgatcag cgcgcaagct gcccggccaa ggatttccgt gacctgcgcc
     4801 agcggcacgg catggtgccc atcgcccagc cggcagatca gatagggctg gcgcgcggcc
     4861 ggatcggccc cggcagacac agcagggcca ccagccggcg ccagaacgcc cgcggccggc
     4921 gggcgttctt ccagatgcga cagacgcacc agttcgccat gggccaccag cgcatcgcca
     4981 tccagcagca gcatcggcac agcagcggcc ccctccggcg acgccggcgc cgccggcagc
     5041 aggccgccga tcagccccgc ccggccaaag gccaggccct gcatgcccgc cgcctgcccc
     5101 gcgcgcaccc gcaccatatc ccgcaccgca tcgatgcgca gcgccacccg ccgcccctcg
     5161 cccatgcgca gcagcaccgt ggctatggcg cgggcgggcg gagagcggcc cagccccagc
     5221 agccgcagcg tatccaccac cggcacctgc acgccgttat attccacccg cgcctgccac
     5281 agcccgtctt ccaccgggct gggcgccacc gggcggcagg gcaccgtggc ctcgatcacc
     5341 cgcgccggca ggcccaggct gagatcgccc gccgccacgc acagcaccgg ctcgccgcgc
     5401 tgggccttgg ccatggccga aaacagcctg tcctcggcca tggcgatgcc cggcagggcc
     5461 gccagccccg ccatatcgag cagcaccccg ctgcggccat tgctgacaaa accggcgccg
     5521 accaggccgc cccccggcgc ctctatgccc ggcgccactt ccatccgcgt caggctttcc
     5581 ggccccagcc ggatcacccc gcagatctcg tgcaggccca cgcccaccac ccgatcgccc
     5641 cgccccggat cacgcaggcg caccaccatg atgatgcccg gccgctgcgc ctctggccct
     5701 gcctctggcc ccgccgcccc cagcagcgcg gccagatcca gcaccggcac caccacgccg
     5761 cgcaggtcta tcgcgcccag cagctgcggc atggtggccg gaatggccgc cagcgccggc
     5821 ggatagggca ccacctcgcg gatgctgtcg atcgccaggg caacatacat cgcgccaatg
     5881 cgcgccatgc cgaacagatc ggcggcgccc ggcccgtcac tggcgcccgg cccctcgctg
     5941 gcaggcgccc cctcgctggc cggcgccggg gcgctcatgt ccatcgcgcg ctcaatcggg
     6001 ccggcgcgag gcagtggcgg ccaggcgctg gatcagcacc accacctcgg ccgagacctg
     6061 atcctgggtg gcggcagagc ggctgatatc ctcgatggca gagccggtct tgtgcacggc
     6121 atcgacaatg ccggtgaagg cctgcgaggc ctcgcgcgag cgatccgtgc cctggttgat
     6181 gcgctggatg gactggtcga tcagccggct gatatcgcgc gcggcggtgc tggaacgttc
     6241 cgccagcttg cgcacctcgc cggccaccac gctgaagccc acgccatgtt cgcccgcgcg
     6301 cgccgcctcg atctcggcgt tgaaggccag caggttggtc tgcccggcaa tctcgccgat
     6361 aatgccgaca atctcggcaa tgccggcggc cgaggtctgg atcagctcga tcgcctcgat
     6421 cgccttgccc agcgcatcgt gccctttttc cgcatcggca tgggtggagc gcgacagccc
     6481 gtcggcggca atcgtggccc cgctgatcgc cgagatcgac gaggagagcc gttccaccag
     6541 cccgcccaga tcgcccgcgc gggcctcgat ctcgcgctcc agcagcacct ggtcggtaat
     6601 gtcgaaggca tatttgacga tccgcgccac cttgccctgc agatcgagga tggggttgta
     6661 ggtggcctgg atccacacgt cgcggtcgta tttgcccacc cggtggaagc ggccggcatg
     6721 atattcgccg cggttcaact tcagccagaa atcggcatag gcgcggctgc gcacataatc
     6781 gggcgagcag aagatggcat ggtgctggcc caccacctcg cgcagcgaat agcccatggt
     6841 ggagaggaaa tgctcgttgg ccgagaggat gttgccatcc agatcaaact cgatcaccgc
     6901 cagcgagcgg ccaatcgcgg ccacccgcgc ctgatattcg ctggccgcca gcttggccgc
     6961 ggtgatgtcc gaggcgattt tgcacacttt caccggcttg ccatcggcgc cgttgatggg
     7021 gttgtagctg gcctggatcc agaaatcctc gccgcccttg cggcgccggc agaattcgcc
     7081 cgccacatgc tcgccgcgcg ccagccgttc ccagaaattg gcataatcgg cagaagcggc
     7141 catgtcgggg tcgcagaaca ggcggtgatg gcggccctgg atctcgtcga gccgatagcc
     7201 ggtgacggcg aggaactggg cgttggcggt gatgatgttg ccggccagat cgaactcgat
     7261 catcgcatgg ctgcggtcga tcgcggcgat cttctcggcc tgctccaccg ccagctcgtg
     7321 gctctgggtg atgtcgtgag caaatttgac gatcttggcg acatggccat ccagatcgaa
     7381 caccggattg taggtggcgc ggatccacac atcgcgcccg ccgcgcccct tgcggcggaa
     7441 gatgccgctg gcaaattcgc cgcgcgccag cttctgccag aaagcggcat attcctcgct
     7501 ggcgacaaag cccggatcgc agaacatcgc atggtggcgg cccagcacat cttcggccgc
     7561 atagcccatg gcggccagga aattggcatt ggcgctcagc acattgccgg ccagatcgaa
     7621 ttccaccacc gcctggctgc gatcgatggc ggccagcttg ctgtcggcct cgatggcggc
     7681 gagacgctgc tgcgtcacat ccatcgccac cttcatgatc ttgtaggggt ggccatcggc
     7741 atcgaggatg gggttgtagc tggcctggat ccacacctcg cgctgatcct tgcggatgcg
     7801 cttgtattcg ccctgatcga actcgccgct gcccagcttc ttccagaact ggcgataggc
     7861 cgggctggtg acatagtcct tgtcgcagaa gatccggtga tgggcgccct tcagctcgtc
     7921 cagcgtatag cccatcagcc gcaggaagtt ggcgttggca tccagaatgc ggccttccag
     7981 gtcgaattcg atcagcgcaa aggcccggtc gatcgccagc aggcggctgg cgctgtcggc
     8041 ccgcgcgttc tgctcctcgg tgatgttgat cgcataggac agcaccgaga ccagccggtc
     8101 gccatccatc gtcggcacaa acacctcgcg cagccacacc tcctgcccgg ccgcattgcg
     8161 atgcttgcgc accagcagat catgccgccc ttgcgcaaag cgctgccaat aggcggccgg
     8221 atcggcgggg caatccgcgc catgccacag ggcggtgaaa ggctggccca ccagatcggc
     8281 gccggccatg cccacgctgg cgcaaaaatg atcgttggcg gccagcaaaa tgccatcggg
     8341 gctgtattgg caaatcgcca gcgcatggtt gatcgcctcg aagcgatggg taagcagggc
     8401 cacatcgccc gcgccctggc agctgcgcac gcccaggaaa tggatcagcc cctggcggtc
     8461 ttcggcccgc atggccatgc cgtgcagacc cagaaacgtg ccatcggccc ggggcaggcc
     8521 gccgctcagc accagccgct cgccagccag cagccgttcc cacagcgccg gcgcatcggc
     8581 aaacaggccg gcaaaggcgc aagtggccag atcgccgccg cccagcccgg ccagcgcttc
     8641 ctcattgccg gccaccgcca gcccgctttg cgcatcaaac gccaggatga tcgccgcatc
     8701 ggcaagcagg gccgccgtct tgccgaccag atcaagaccc aggggctgca tcgtcattct
     8761 tgtctctccg cacggcaggc ccgctgtgcc gccctgcggt gcctccttaa ggagcggaag
     8821 ttaatttggc cgccgccaga accgaaggaa atcccctaat cccttccttt tcaagctgct
     8881 aaccacaagg cgcgcgccgc ctgggctgcg ctgcgcaccc ggaacaaagg gccagaaaca
     8941 aggcgttgga cgaaaggcgg caattcctgt agggcaaggc taccgtcagc tttctccttc
     9001 aagcgctgcc ggctgagagt tctcatgctc ccgcttggtt cggagcatgg ccgatgaccc
     9061 ctcaccgcat gggccccacc accaccgctg ttcccgatgg caaccgggcc gcactggcca
     9121 ccgcccatgc cgcgctgcgc cagggctgcc acttcctcag cgcccgcagc gagggggacg
     9181 gatcgggcgg gcatcgccat gcccggctgc gctggcgggt ggcgggcaac catctgaagg
     9241 agctggatcg ctttctggcg gtgctgctgg ccgaatatgc gcaggccacc ggccatgccc
     9301 gcccgcccct cacgcccccc atccccgaca gtctggcccc cgcccccgac agcgccgagg
     9361 cg
//
LOCUS       DASRYE010000011        34758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_124174,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000011 DASRYE010000000
VERSION     DASRYE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 34758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34758
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124174"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1762)
                     /locus_tag="VFF98_00315"
     CDS             complement(113..1762)
                     /locus_tag="VFF98_00315"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HZU62097.1"
                     /translation="MTMTATDAATAIATTPFAGQKPGTSGLRKKVKVFAQPHYAENFI
                     QSVFDVVERPAGATLVIGGDGRYHNRQVIQTAIRMAAANGYGRVLVGQGGILSTPAAS
                     HIIRQYGASGGLVLSASHNPGGPEEDFGIKYNIANGGPAPESVTEAIYARTQEIARYL
                     TVEAPDIDLDAIGTQQVAGLAVEVIDPVADYAALMERLFDFGAIRALVADGFAMTFDA
                     MHAVTGPYAREILEKRLGFAPGTVKNGVPLEDFGGHHPDPNMVHAADLFAALFGEGAP
                     DFGAASDGDGDRNLILGRGIFVTPSDSLAMLAANAHLAPAYATGLKGIARSMPTSGAA
                     DRVAARLGIPLYETPTGWKFFGTLLDAGLATICGEESAGTSSDHVREKDGLWAVLLWL
                     NILAVRRQPVAALAAEHWATYGRNYYARHDYEALPTEAANALIAELTASLGSLPGQSF
                     GPLVVAEADSFAYTDPVDGSVSRNQGLRVLFEGGSRIVFRLSGTGTEGATLRVYLERY
                     EPATGRLAEETPAMLADLVAAADAIAGIVRHTGRTGPDVIT"
     gene            complement(1759..3261)
                     /gene="glgA"
                     /locus_tag="VFF98_00320"
     CDS             complement(1759..3261)
                     /gene="glgA"
                     /locus_tag="VFF98_00320"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZU62098.1"
                     /translation="MVLRVLSVASEAVPFIKTGGLADVAGALPAAVAPHGVAMSTLLP
                     GYPQVMARLSAGAFRVLHEWPALMSAPAPPIPARLLLAEPAEEGLAGPLLVLDAPALF
                     VRDGGPYVDTAGRDWADNALRFAAFGRAAADIAGGAVTGLAFDVVHAHDWQAGMALAY
                     LRFAPPVSARPGAHVPPGVMTIHNMAFQGHFGGDVFPQLGLPQAAWAMDGVEYHGGVG
                     FLKAGLEAAARITTVSPTYALEIRSPQFGMGLEGLILSRGDAVRGLINGIDTREWNPA
                     ADPALAAGFSARNFNRRVLNKRALEAEFGLEHEDGPLFIAISRLTWQKGMDVLAECVD
                     QLVQMGGRLALLGSGDAAIVGQLHAAAARHPGRVAVRIGYDEGLSHRMQAGGDAILVP
                     SRFEPCGLTQLYALAYGCVPVVARTGGLADTVVDANLAALKAKVATGIQFDRVDYSGL
                     AGAIARAIHLYRQADAWRSLQKNGLRADFSWASSGGAYASLYREIAPETA"
     gene            complement(3271..4530)
                     /gene="glgC"
                     /locus_tag="VFF98_00325"
     CDS             complement(3271..4530)
                     /gene="glgC"
                     /locus_tag="VFF98_00325"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZU62099.1"
                     /translation="MRDSFGSPLAREAMAYVLAGGRGSRLKELTDNRAKPAVYFGGMS
                     RIIDFALSNAINSGIRRIGVATQYKAHSLIRHMNRAWNFLRPERNESFDILPASQRVS
                     ETQWYEGTADAVYQNIDIIEAHGVRYMVILAGDHIYKMDYEHMLRQHVNSGADVTIGC
                     LVVPKKDASGFGVMAVDTNSVITDFVEKPAHPPEIPGQPGFCLASMGIYVFETQFLFE
                     RLREDAADPASSRDFGKDIIPRIVKHGKAVAHRFSESCVRAAEESEEYWRDVGTLDAY
                     FEANLDLIDVVPALNMYDREWRIWTDQVVASPAKFVHDEEGRRGMAISSLISQDCIVS
                     GAVARRSLLFTGVKMGSWSSVQEGVILPYCNIGRGARLSRVIVDSGVRIPEGLVVGED
                     PELDAKRFRRTENGVCLITKPMIDRLK"
     gene            complement(4549..6717)
                     /gene="glgB"
                     /locus_tag="VFF98_00330"
     CDS             complement(4549..6717)
                     /gene="glgB"
                     /locus_tag="VFF98_00330"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZU62100.1"
                     /translation="MKPPEEAITALLEGTHADPFALLGCHAGPHGTFARVLLPGAEVA
                     EAFSLAGEPLGALALTDGRGLFEGAIAGPPGPVKYRAGAKGTQWWVTDPYSFGPVLGP
                     LDDYLIAEGSHLRLFDKLGAHLITHEGAQGVHFAVWAPNARLVSLVGDFNDWDPCRHP
                     MRRRADIGVWEIFLPDIGAGRAYKYRILGADGTLLPLKADPFALASELRPATASLTAH
                     PVKQDWGDAGHRAHWASVDPRRVPVSIYEVHAGSWQRDENGWFLNWDKLAERLIPYVA
                     DMGFTHIEFLPVSEHPFDPSWGYQTTGLYAPSARFGPPEGFARFVDGAHRAGIGVILD
                     WVPAHFPTDAHGLARFDGTALYEHEDPRQGFHPDWHTAIYNFGRREVASFLVNNALFW
                     AERYHVDGLRVDAVASMLYRDYSRENGEWVPNESGGRENWEAVDFLRSVNRAVHAAQP
                     GILTIAEESTAWPGVTQPAYEAPGRPAALGFGFKWNMGFMHDVLRYFGRDPVHRAWHH
                     EDVTFGLMYAFAENFVLALSHDEVVHGKGSLLAKMAGDDWQQFASLRALYGLMWGYPG
                     KKLLFMGQEFAQRNEWSEARGLDWDLLAAPAHEGVRMLVRDLNRIYRETPALHARDCE
                     GEGFAWLIVDDRHNSVFAWARRAPGARPVAVIANCTPAPRTGYRVPLPADGRWVEIVN
                     TDATAYGGSGMGNGGEVWATDGGAHVTLPPLATIMLEYAG"
     gene            complement(6714..9119)
                     /locus_tag="VFF98_00335"
     CDS             complement(6714..9119)
                     /locus_tag="VFF98_00335"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZU62101.1"
                     /translation="MELAAPPIRALILDVLRHRVGKDERAAKAHDWFSAAALALRDKV
                     IDRWMESTRRTYESGGKRVYYLSLEFLIGRLMRDCLSNMGLTREMEGALREHGFELAQ
                     LEELEPDAALGNGGLGRLAACFMESLASLDIPAYGYGIRYVNGMFRQRIDDGWQVELP
                     ETWLAHGNPWEFDRRESAYRIGFGGEVVEDNSGGVTWHPHEQIEASAIDTPVVGWRGK
                     RVNTLRLWTAWALDPLRLDAFNAGDHFGAMAEQVKATSLVRVLYPADSNPAGQELRLR
                     QEYFFTSASIQDILRRHVQYHGDVRSLPEKAAIQLNDTHPSVAVAELMRLLIDVHGLE
                     FDEAWEITKQTIAYTNHTLLPEALESWPLPLFERLLPRHMQIVYAINSRVLREARKAG
                     MDGAQIAAISLIDEGGERRVRMANLAFVGAHSVNGVAALHTELMKSTVFADLHRLYPA
                     RINNKTNGVTPRRWLQQCNPGLTALIRDGIGEAFLDDTTRLADLNALAEDSDFGERIA
                     EVKRANKVALSNHLKALMGVRLNPDALFDVQIKRIHEYKRQLLNLVETVALYDQIRSH
                     PEKDWVPRVKIIGGKAAASYHNAKLVIKLANDIARRVNSDPSVGGLLKVVFVPNYNVS
                     LAERIIPAADLSEQISTAGMEASGTGNMKFALNGALTIGTLDGANIEIKDCVGDENIF
                     IFGLTADEVAAKRADGYHPRALIEQNRELRQAVNAIASGVFSPDDPDRYRDLMGGLYD
                     HDWFMVAADFEAYAATQRAVDARWEDQASWRSAAIRNIANVGWFSSDRTIGEYAKDIW
                     GVS"
     gene            complement(9419..11512)
                     /locus_tag="VFF98_00340"
     CDS             complement(9419..11512)
                     /locus_tag="VFF98_00340"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZU62102.1"
                     /translation="MLRQYELVERVLAYAPDADEAMLNRAYVYTVQKHGTQKRASGDP
                     YFSHPVEVAGLMTELKLDQDTIITALLHDTVEDTLATTEEIEKLFGPDVARMVDGVTK
                     LSKIEAMSEKERAAENLRKFLLAMSEDLRVLLVKLADRLHNMRTLHFIKSEDKRRRIA
                     RETMDIYAPLAERVGMYEYMREMQLLAFQQLEPEGYATITGRLAQIRESDGGQVDAIA
                     LAISQTLTNAGLRCKVWGREKHPYSIWRKMAERHVNFEAITDIMAFRVLVDNVEDCYR
                     AIGTLHTAWQAIPGRFKDYISTPKANGYKSLHTSLIYEKAMRMEVQIRTWDMHETNEY
                     GLAAHWSYKQGGIKPDGQVGWLRDLLEVLDSSHDPEELLENTKMAIYQDRIFAFTPKG
                     ALFQLPKGATPIDFAYAVHTDLGSMAVGAKINGRHMPLRTPLGNGDVVEIIKSRSASP
                     QLSWLGFAITGKARAAIRRAVRAKERAEIAAIGRKLFDDIAAHLPTKVGKKAIGDALK
                     RLNLKSEDELMAAIGTAKLDDRQVMEALVPGSTSALPPADHWPRHDRTISIRGLTPGI
                     AFQLAECCHPVPGDRIVGLRKPGVGVEVHAISCETLAGGEDREWLDLSWGEASKGAVS
                     RLRVLLYNRPGTLAEVTGIFARNHANIVSLKMAGRDELFGTYEVDLEVHDLAHVSRLV
                     SALRASEAVAQAERL"
     gene            complement(11606..12151)
                     /locus_tag="VFF98_00345"
     CDS             complement(11606..12151)
                     /locus_tag="VFF98_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZU62103.1"
                     /translation="MSRARRSSDWGFPRWRGYESAREAAKVRLCDRAGCTEPGNCPAP
                     KSPNSPERWYFCQAHAAEYNAGWDYFAGLDAEEAASRRKAEEQTSAGYRESAYYEWAG
                     SGDGTRSRDEMRALEVLGLDNDADFDAVKKAWRSRAKEVHPDVRPNDAEAAKAFQALQ
                     LAYEVLRAAEERRQWRGPQAS"
     gene            complement(12295..12822)
                     /gene="pal"
                     /locus_tag="VFF98_00350"
     CDS             complement(12295..12822)
                     /gene="pal"
                     /locus_tag="VFF98_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZU62104.1"
                     /translation="MLSRFPRNRTLILLAVSATALAACAPKPPKQLPPEPGPTVAAAP
                     APAPVPSGPTPGSQADFLASIGGRDTIHFAFDKYNVDDLSVEILKTQAAWLLKYPAKK
                     ATIEGHADERGTREYNIALGERRATAAKNYLVSLGVDASRLTTISYGKERPVDPASTE
                     EAWAKNRRAVTVTID"
     gene            complement(12903..14321)
                     /gene="tolB"
                     /locus_tag="VFF98_00355"
     CDS             complement(12903..14321)
                     /gene="tolB"
                     /locus_tag="VFF98_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZU62105.1"
                     /translation="MTWKFDRLTSPLAAAMLLLAPLLAPVPAGAQDLPPAPAAAPPAP
                     APAAGDQGLSVTVSDDSAWQSLGIDIPAFATPADVPTAASAGSTAQLGRAIAEVITAD
                     LKNNGLFKPVGPGQLPGVPMEQVQAPDFAAWSARGAEMLVHGAVSAGADGQLQVGCYL
                     YDVALKQQRVGDIWNFVAADWRRAAHKCADAIYARLSGESPFFDSRIAYIAETGPKEH
                     RMKRLAIMDSDGANHRFITSGQAMALTPRFSPDYKKIVYLSYLNGAPRIYVYDVAQNT
                     QRLVVSTTNPVIAPRWSPDGKWILYSMSIAGNTNIYRVPAEGGRSQQLTDTPGINVGG
                     SFSPDGGKVVFESDRSGSQQIYVMNADGTDQHRISFFGGKSATPEWSPRGDQIAFTHI
                     GGNLRIAVTSPGGQGLRYLTDSWQDEAPTWAPNGRIIQFFRTEKGAGHTSIWQVDLTG
                     RNERRLPTPVDASDPSWGPVRP"
     gene            complement(14318..15178)
                     /locus_tag="VFF98_00360"
     CDS             complement(14318..15178)
                     /locus_tag="VFF98_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62106.1"
                     /translation="MARLLSRGDERIALTVAVVAHVALLAALVLAPARKAPPTPQRMA
                     VTLADDVGPVSTSPHPEQPAAEDLAPTLGETTPPPQPAPEPQPQPKPQPVPQPQPRPA
                     PVPRPQPHPQPQPKPQPAPRAEARPRPDAFRDAFAKTAEEPHKANRPVGGSRIGADFL
                     KGVTSSQSSTRGQGAPAQAAGPAVRAAIGGAIARQLKQHWRVPQGVDTDQLVTVLAFD
                     LNRDGTLAGPVRVVRQEGITDSNRPQAGLHRDNAIRAVQLAVPFQLPPEYYDTWKHLT
                     AVRFDRNLSQ"
     gene            complement(15204..15692)
                     /locus_tag="VFF98_00365"
     CDS             complement(15204..15692)
                     /locus_tag="VFF98_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU62107.1"
                     /translation="MAMGLHRQGQRGRGSRRAPMAEINVTPLVDVMLVLLIIFMVTAP
                     LLKAGIPISLPDSKAHSLDHQPKEITITMTRDGNVFLDDAPLAPGELEDRLANLPRDA
                     QGQAPLVTLRADKALDYGQVVSLMGELNAAGISQISLVTVAAGGGAGQARPVGARPVS
                     AR"
     gene            complement(15692..16396)
                     /gene="tolQ"
                     /locus_tag="VFF98_00370"
     CDS             complement(15692..16396)
                     /gene="tolQ"
                     /locus_tag="VFF98_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZU62108.1"
                     /translation="MTDLLLLAAGSAAPAHLDPIKLFRDADIVVQVVMAGLVLASIGV
                     WTIIFSFSWRMGALRRRTAAYERDFWQAQDIDRFQAEKSGGRDLPIGKVVSAALGEWR
                     RSANGKVDREGARQRLATAMGVVVAQEADELAGRLTFLATVGSVAPFVGLFGTVWGIM
                     NSFFQIGQAQNSSLAVVAPGISEALFATATGLFAAIPAVIAYNRFSAGVNLFEAALQR
                     FADKFHTSLSRELDQL"
     gene            complement(16393..16866)
                     /locus_tag="VFF98_00375"
     CDS             complement(16393..16866)
                     /locus_tag="VFF98_00375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HZU62109.1"
                     /translation="MSMFPDPPAGLFDGPVHRYAVRAYFEDTDLSGVVYHANYLRWFE
                     RARSDMLRLLEIDQRAAWQAGEGAYAVAEMHLRYLAPARLDDAVLIESRVVDMRAASC
                     RMHQLAFRNGTLLADARVRIGFVGPDGRARRQPDGWRAAFSSLLSSSSSSTTGPQ"
     gene            complement(16922..17314)
                     /locus_tag="VFF98_00380"
     CDS             complement(16922..17314)
                     /locus_tag="VFF98_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZU62110.1"
                     /translation="MPIDPSQPYDPNNIFARILRGEIPCKKVYEDDHALAFHDIAPQA
                     PVHVLVIPKAAYVSWEDFSARAPAEEIGHFVRAVGQVARQLGLAGEGSAWPGYRLLAN
                     VGGDGGQEVPHLHVHLFGGQPLGPMLAR"
     gene            complement(17355..17684)
                     /locus_tag="VFF98_00385"
     CDS             complement(17355..17684)
                     /locus_tag="VFF98_00385"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZU62111.1"
                     /translation="MSDTDLAILARLEGLIAQRRAASPETSYVARLHAKGVKKIAQKV
                     GEEAVEAAIAAVADDRAELVGEAADLLFHLLVLLGARQIPLAEVCALLEQREGTSGIA
                     EKASRSA"
     gene            complement(17810..18574)
                     /gene="hisF"
                     /locus_tag="VFF98_00390"
     CDS             complement(17810..18574)
                     /gene="hisF"
                     /locus_tag="VFF98_00390"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZU62112.1"
                     /translation="MSVRIRVIPCLDVRDGRVVKGVNFVDLVDAGDPVEQARAYDAAG
                     ADELCFLDISASHEGRGTLLDVVRRTAEVCFMPLTVGGGVRSAEDARALLLAGADKVA
                     VNSAAVSRPEVVSDIAEKFGSQCIVASVDARRVAEGRWEIFTHGGRRPTGIDALEHAQ
                     KLARLGAGELLVTSMDGDGTQAGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIE
                     GHASAVLAASIFHFGKHTIAEAHAALRASGLPARQH"
     gene            complement(18578..19309)
                     /gene="hisA"
                     /locus_tag="VFF98_00395"
     CDS             complement(18578..19309)
                     /gene="hisA"
                     /locus_tag="VFF98_00395"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZU62113.1"
                     /translation="MIVFPAIDLKGGQVVRLAEGDMARATVYGDNPAHQAMLFAQAGS
                     QYLHVVDLDGSFAGRAENRAAVEAILQEFPGHVQLGGGIRTAAAVEGWFDLGVSRVVM
                     GTAALKDPQFVKDMARAFPGGIVVAVDARDGFVATEGWADVSDVAVVDLARRFEDAGV
                     ASLLFTDIGRDGLLKGANIEATVDLARAVAIPVIASGGVKGLDDIRLLSLHAHEGIEG
                     VITGRALFEGRLDLAAAIALAAKEG"
     gene            complement(19306..19935)
                     /gene="hisH"
                     /locus_tag="VFF98_00400"
     CDS             complement(19306..19935)
                     /gene="hisH"
                     /locus_tag="VFF98_00400"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZU62114.1"
                     /translation="MADRLALIDYGAGNLHSVANALAAAGADHVAITADPDVVRSADR
                     IVLPGVGSFRACAEGLRAIPGLVEAMDARVRGDGVPFLGICVGMQLLATRGVEHGITR
                     GLGWIPGEVRLIAPEDPAIKVPHMGWNDVVVAQGAPAGGLIVPGEAYFLHSYHFVPEE
                     AGHIAATTAHGAPLVAAVARGNIVGVQFHPEKSQAYGLGLLGRFLEWMP"
     gene            complement(19928..20536)
                     /gene="hisB"
                     /locus_tag="VFF98_00405"
     CDS             complement(19928..20536)
                     /gene="hisB"
                     /locus_tag="VFF98_00405"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZU62115.1"
                     /translation="MRTARIARKTHETDILVELNLDGTGAYDVATGIGFLDHMIEQFS
                     RHSLIDMKVHIKGDLHVDQHHTVEDCAIALGQCVVQALGDKAGIGRYGQVYSPMDEAL
                     ARVALDISGRPWLVWKVPFSQARLGEMDTELFEHWFQSFAQAAGITLHIELLYGQNNH
                     HIIEGVYKGLARAMRSAVELDPRKGGAIPSTKGLLGDGSLHG"
     gene            20697..21197
                     /locus_tag="VFF98_00410"
     CDS             20697..21197
                     /locus_tag="VFF98_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HZU62116.1"
                     /translation="MSNDTPDSLIPYDEIVQDALRAVVGRVLGQVETTGGSLPGNHHF
                     YITFKTHAPGVDIPAHLKARFPDEMTIVLQNKFWELKVAADHFSVGLSFNQVPAKLVI
                     PFAAITAFVDPAVDFGLQFQASAGELEPEPHEEAENDSPEADAPRTEPAGDGSNVVTV
                     DFGRKK"
     gene            21194..21529
                     /locus_tag="VFF98_00415"
     CDS             21194..21529
                     /locus_tag="VFF98_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62117.1"
                     /translation="MSGAGRGKDAAPPAPDSARLLADVLKEQGHKLIDRTVGRVLPGA
                     AAATPPPEAPTKPSSPAERLARRVAGAALMRIASASVPGAILVGGGLVAAHLHRRQRA
                     RRAREADQG"
     gene            21604..22275
                     /gene="gmk"
                     /locus_tag="VFF98_00420"
     CDS             21604..22275
                     /gene="gmk"
                     /locus_tag="VFF98_00420"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZU62118.1"
                     /translation="MAHTDPTLSGKPADTLHRRGLMLILSSPSGAGKTTIARRLLDVD
                     TEIAPSISATTRPPRPGEVDGKDYYFIDQAEFDRMAEASEFYEWATVFGKSYGTPKAH
                     IREGLKQGRDFLFDIDWQGTQQLSQKAGTDVVRVFILPPSLGELRRRLTGRGTDSAEV
                     IAQRMARAQAEISHWDAYDYVVINEDVDACFEQVRQILAAERMKRVRQTGLIPFVRAL
                     LHGEG"
     gene            complement(22425..23405)
                     /locus_tag="VFF98_00425"
     CDS             complement(22425..23405)
                     /locus_tag="VFF98_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62119.1"
                     /translation="MASVALAAGLPAAAHANAVSGISGDISVEAGYNTVGQETFTYTE
                     TGGYSNSWNNGYRYGGVSVDLNGSVALPITKSIGAQIDLGLDHNIAHPTGHWCTYAYE
                     CGATKLDATDVAVHLFTRKPSLGLVGVFAQRTSTTNTNYSGYAGATYYFGGEAQLYVG
                     KLTISGQVSAHHTDYQYYKDRGIQTAGRLTYYVNPHFALTAKAGYEYSDYFGRWYQYY
                     SYSDIETYPSHAWNVGVKAEHQLGHSNVSLLARYDYYGFVSSDTYTESNGSYTWRYNY
                     PYHASRVLVGVKVHLGKHVSLEERDRNGAGLDAVDTTFQELRSLGNNYED"
     gene            23726..24436
                     /locus_tag="VFF98_00430"
     CDS             23726..24436
                     /locus_tag="VFF98_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZU62120.1"
                     /translation="MERPAPTPAAPRTIPLSLFAASLLYGGLCVLAGVLGTKIADLGQ
                     WPVVGHLAVESGIFAFLTLVVLASATAELHGTATANRLVRLGFVPLIVSMLLLMLVIH
                     VVPPAPFWHDQEAFARLLGQGARMQLAGLVSYGTSQTLNVAVFARLAGRGGHGSALWL
                     RAWIASLLSQAVDTMLFITISFAGVHDAATGQPIPILTLMTGQIIAKMLLSTLMVPVL
                     IQLAVLLGRKLDAPCENV"
     gene            24447..25376
                     /gene="argB"
                     /locus_tag="VFF98_00435"
     CDS             24447..25376
                     /gene="argB"
                     /locus_tag="VFF98_00435"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZU62121.1"
                     /translation="MAHPQEPALSLDSLLAKAETLIEALPYLKRYEGRTFVVKYGGHA
                     MGDPALAQDFAEDIVLLKAVGINPVVVHGGGPQIGRMLKALGIESQFVNGLRVTDKAT
                     AEVAEMVLSGAINKELVSWIAKAGGRAVGISGKDGALVTARKVSAQKLGKAVPDPESG
                     EAIVVDLGYVGEPDRIDTSMLDTLSGAGLIPVIAPIAVGNDGETYNINADTMAGGIAA
                     ALGAARLFLLTDVPGVLDKNKTLLTDLTPADIATLAEDGTISGGMIPKLETCVHAVEA
                     GCEAAVVLDGRVPHAMLLEFFTSRGAGTLIRKG"
     gene            25564..25854
                     /locus_tag="VFF98_00440"
     CDS             25564..25854
                     /locus_tag="VFF98_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZU62122.1"
                     /translation="MLYTLIDILNYLIELLRGIVIVQFVLSLLISFNVVNLHNDLVQA
                     IWRAVNVLLEPFLRPLRRIMPDTRPIDFSPMALIVLLSIVQIVLGHLAFSAL"
     gene            25880..26794
                     /gene="folD"
                     /locus_tag="VFF98_00445"
     CDS             25880..26794
                     /gene="folD"
                     /locus_tag="VFF98_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZU62123.1"
                     /translation="MTDTHVAPTAGALIDGKAFAAGLRARVGDLAARFTAEAGRKAGL
                     AVVLVGADPASQVYVGAKHKATVAAGMESFEHRLPADTSQADLLALIEQLNTDPAVDG
                     ILVQLPLPRHLDEQQVIATISPDKDVDGFHVTNAGRLAVGQPGFVPCTPLGCLMLLQD
                     RLGDLAGKEALVIGRSNIVGKPMAQLLLQADCTVTIAHRHTEALAEVCRRADILVVAV
                     GRAEMVKADWVKPGATVIDVGINRIHTADGKTRLVGDVDYAGVAQVAGAITPVPGGVG
                     PMTIAVLLRNTLVAAFRNAGLPLDESAI"
     gene            26794..27645
                     /locus_tag="VFF98_00450"
     CDS             26794..27645
                     /locus_tag="VFF98_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62124.1"
                     /translation="MGSLGGRGQQPGRAPAALGLAALLLLAPPALAAPPLALPPQEEP
                     HATGQLVEGLAAGEPSEVFSAELQMKLRARAKGDRDAIKRFASPEAQVADARQPGHAP
                     LAGRQPPALSQRSAFLAWVSCDGTVALTQGRLPAAGVGEAHSRSYLTVWQRQRKGTPY
                     RFVLDLVSQQGQSPADPDFAEGHVAQCPLHRRGDAGPVGKPGKPSKVQPPAFTADTPE
                     RLTGEAQDHTLRWELTTPADGAPFFALYEKIASAPKEAPAYRPVLALGSLRAAAPLLP
                     PTPAPTL"
     gene            27687..28310
                     /locus_tag="VFF98_00455"
     CDS             27687..28310
                     /locus_tag="VFF98_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZU62125.1"
                     /translation="MVDLFVSAFFTLFVVIDPIGCAPIFAGLTAGTPAAHARGVALRA
                     SLIASLILLAFALGGKPLLAALNIGLDAFRIAGGIMLFLIALEMVFEKRTQKREERAE
                     KALHTPPAEDISVFPMAMPMIAGPGSIATVMLMMGRAQGLAGGAVVLAALACVLAITL
                     VALLAARPIMALLGSRVEAVITRVLGVLLGALAVQYAIDGLRGVFGG"
     gene            complement(28327..28935)
                     /locus_tag="VFF98_00460"
     CDS             complement(28327..28935)
                     /locus_tag="VFF98_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZU62126.1"
                     /translation="MARISIFPLAGAVLYPGLRLPLHIFEPRYRALISDSLARDRRIG
                     MIQPQSGEEGAPLYHVGCLGHIEDVEALPDGRFNIVLEGLARFRVGRELVVSTPFRQV
                     EAELFDEPEAEALAAVERALFEREAHRFADAHGYRVDWEQVQRLDDAALINGVAQIAP
                     FDAASKQVLLEAPKLAERCELLVQLMQFYGKRDSDDNQATLQ"
     gene            complement(28947..29852)
                     /locus_tag="VFF98_00465"
     CDS             complement(28947..29852)
                     /locus_tag="VFF98_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU62127.1"
                     /translation="MASLGLNLDERKAVERFRQTVVEPSMESLVILDFWAEWCGPCKA
                     LTPVLEKVAADYADKGVILAKINVDEEKFIASQFQVRSIPTVYALFQGQPVADLTNAR
                     SESQLKGLLDQLLKQLPVKAGAGAPDIEPLLAMGEEVLDGGDAERAVSIFAQILEMAP
                     ESPAAHAGLIRALIVMGHLEEAEAALAELPEGAGADPAIERARAALALAKDAPEAGEL
                     AQLKAAAEAAPADQEAGFAYANAAFAAGERDAAAATLLRLIAADRAWNEGAARSRLLQ
                     IFEVIGLEDPWVAAQRRKLSTILFG"
     gene            30139..30213
                     /locus_tag="VFF98_00470"
     tRNA            30139..30213
                     /locus_tag="VFF98_00470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30171..30173,aa:Gly,seq:gcc)
     gene            30252..30326
                     /locus_tag="VFF98_00475"
     tRNA            30252..30326
                     /locus_tag="VFF98_00475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30284..30286,aa:Gly,seq:gcc)
     gene            complement(30378..31394)
                     /locus_tag="VFF98_00480"
     CDS             complement(30378..31394)
                     /locus_tag="VFF98_00480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZU62128.1"
                     /translation="MLRDEILAMPSYAQLLARADAPAGSSWGLFGPADQLGTLNFLSR
                     DSVLGGVLAVRSGEAFSLDLRSDAISPSLAPTRQPLEHHIFQRNDFHHDEWIDKLYTQ
                     YGSQIDGLRHIGHPDHGFYNGYDPAEFKPGTELLGIHHFAAAPIAGRGVLIDLARYCA
                     EVLGAPIDHLKGQAIPVALVDEACAAQGAEICPGDIVILRFGWLDYYRHQAPEEIRAN
                     LVQNQFHPGLLQSHDTVAWLWDKRISLIASDNFALECWPAVPDSPFFTREERETGVHG
                     AHAGIMHRAILPLLGLPIGELWDVDALAEACARDRRYDFLLTVSPMPLVGGVGSPANA
                     IALR"
     gene            complement(31457..32236)
                     /locus_tag="VFF98_00485"
     CDS             complement(31457..32236)
                     /locus_tag="VFF98_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU62129.1"
                     /translation="MDDGKDRDYVQSVERCLRVMQAFADGPRFLGLPELAAKTGLSKP
                     TVRRMLLTLKQLGFVTAADAKFALTARMLDLGYAYLSSVDLPAIAQPLMEELTEELKA
                     STALVALDGDDVVYMSRVHRHRISSISLPLGARLPAYVTASGRVLLAGLNDERLEDYL
                     GHARLEPLTFATIADPAALRARLRQVREQGWATVDQELEIERRSAAAPIRDADGHIVA
                     ALSLSCSTLEATMAYMVEQVVPRLVSAAQRISEGLGRPSSR"
     gene            32391..33173
                     /locus_tag="VFF98_00490"
     CDS             32391..33173
                     /locus_tag="VFF98_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62130.1"
                     /translation="MEPDRLKLILLDLEQTLLAFIRKHGINHAEYRRATDLLIATIKE
                     GEESLLPDVFFEAETTNIGNLQRAGSGEAIEGPFYFPGAPLLTSPARLPQRDNEAGDI
                     LFFSGRVTTPAGDPVPGAEIDVWQADARGLYSQIHPGIPDWNLRGRLRTDEEGRYALQ
                     TIMPPPYEIPKNGPTGRVLAALGRHFFRPAHLHVKLRHANLGEMTSQLYFHGDPYLES
                     DVANAVRDDLIVDLVRHASQQALADRKLDQPFFEASYDFVLG"
     gene            33288..34412
                     /locus_tag="VFF98_00495"
     CDS             33288..34412
                     /locus_tag="VFF98_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HZU62131.1"
                     /translation="MSRIDSITTQRLDIALRQPITMSTGTVSRQNILLVRLRDTDGAE
                     GVGEALVMGGPYWGQETIEGVRAVVERHLAPLLIGQSTSSVGAISRQLPRWVRGNAAA
                     RSAVEMAMLDLVGHRSGLPVSALLGGAVRSRIPVAWTLSTGSVETDIAEGERAAAAHG
                     FTRFKLKFGHEAPRADVSRAHAIARHFAGRATIIADVNQGWDEQTAIQMLPRLVDAGL
                     EALEQPLPAHALAAAAELQRRFGLRFIADEAVDGPASVLRLAQAKAAAALSLKPNRDG
                     GLIATRDAAIIAAAAGIQLYGGSMLETSLGTAANLHCYATAPDLSLGCELFGPMRLGE
                     DIATTPVSIEQGHLTLEDTLPGLGVSLDEEKIAFLAARSG"
     gene            complement(34509..>34758)
                     /locus_tag="VFF98_00500"
     CDS             complement(34509..>34758)
                     /locus_tag="VFF98_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZU62132.1"
                     /translation="WRAYDACALIADGARLPDLLVDQGAADGFLDGQLKTPLLEAACQ
                     AAGIPATIRLQQGYDHSYYFISTFMAEHVEWHAQRMKG"
BASE COUNT         5889 a        11853 c        11328 g         5688 t
ORIGIN      
        1 tccgtatccc cctcgaacag gcacagatcc acccagccgg ccagcggcgc gcgcacccca
       61 aagcgggtgt ggccgcccgc cacgctggcg ccggggggaa tatcgtgcat tgtcaggtga
      121 tcacatcggg gccggtgcgg ccggtgtggc gcacaatgcc ggcaatcgca tcggccgcgg
      181 ccaccagatc ggccagcatc gccggcgttt cctccgccag ccggccggtg gcaggctcat
      241 accgctccag atagacgcgc aaggtggccc cttccgtgcc cgtgcccgag aggcggaaga
      301 caatgcggct gcccccttcg aacagcaccc tgaggccctg attacggctg accgagccat
      361 cgaccggatc ggtgtaggca aagctgtcgg cctctgccac caccagcggg ccaaagcttt
      421 ggcccggcag gctgccgagg ctggcggtca gttcggcgat gagggcattg gccgcctctg
      481 tcggcagcgc ctcgtaatcg tggcgggcat aatagttgcg gccataggtg gcccagtgtt
      541 ccgccgccaa agcggccacc ggctggcgcc gcacagcgag gatgttcagc cacagcagca
      601 ccgcccacag cccatccttc tcgcgcacat gatcggaact ggtgcccgcg ctttcctcgc
      661 cgcagatcgt ggcaaggccg gcatccagca gcgtgccgaa gaacttccac cccgtcggcg
      721 tttcgtagag ggggatgccc agccgcgcgg ccacccgatc agcagcgccg ctggtgggca
      781 tggagcgggc aatgcccttc aggcccgtgg cataggccgg cgccagatgg gcattggccg
      841 ccagcatcgc caggctgtcc gacggcgtca cgaagatgcc gcgccccagg atcaagttgc
      901 gatcgccatc gccatcgctg gcggcgccaa aatcgggcgc cccctcgccg aacagggcgg
      961 cgaacagatc ggccgcatgc accatgttgg gatcggggtg atggccgcca aaatcctcca
     1021 gcggcacgcc attcttcacc gtgcccgggg caaagcccag ccgcttttcc aggatctcgc
     1081 gcgcataggg gccggtaacg gcatgcatgg catcgaaggt catggcaaag ccatcggcca
     1141 ccagcgcgcg aatggcgcca aaatcgaaca atcgctccat cagcgcggcg taatcggcca
     1201 ccgggtcgat cacctccacg gcgaggcccg ccacctgctg cgtgccgatc gcatcaaggt
     1261 cgatgtcggg cgcctccacg gtgagatagc gggcgatctc ctgcgtgcgc gcatagatcg
     1321 cctcggtcac gctttcgggc gcggggccgc cattggcgat gttgtacttg atgccgaaat
     1381 cttcctccgg cccgccgggg ttgtggctgg ccgacagcac cagcccgccg ctggcgccat
     1441 attggcggat gatgtggctg gccgccggag tggacaggat gcccccctgc cccaccagta
     1501 cccggccata gccattggcc gcggccatgc ggatcgccgt ctggatcacc tggcggttat
     1561 ggtagcgccc gtcgccgccg atcaccagcg tggcgccggc gggccgttcc accacgtcga
     1621 acacgctctg gatgaaattc tcggcgtaat ggggttgcgc aaacaccttc accttcttgc
     1681 gcaggcccga tgtgcccggc ttctgcccgg caaagggcgt ggtggcgatg gccgtggcgg
     1741 catcggtggc ggtcatggtc atgctgtctc cggtgcgatt tcgcgataaa ggctggcata
     1801 ggcgccgccg ctgctggccc agctgaaatc ggcgcgcagg ccgtttttct gcaggctgcg
     1861 ccaggcgtcg gcttggcggt aaaggtgtat ggcgcgggca atggcgccgg caagcccgga
     1921 ataatccacc ctgtcgaact ggatgccggt ggccaccttc gccttcagcg cggcaaggtt
     1981 ggcatccacc accgtatcgg caaggccgcc ggtgcgcgcc accaccggca cgcagccata
     2041 ggccagcgca tacagctggg tgaggccgca aggctcgaac cggctgggca caaggatggc
     2101 atcgccgcca gcctgcatgc ggtgcgagag gccctcgtca tagccgatgc gcaccgccac
     2161 ccgcccgggg tggcgcgcgg ccgccgcatg cagctggccc acgatcgccg catcgccgct
     2221 gcccagcagc gccagccgcc cgcccatctg caccagctgg tcgacacatt cggccagcac
     2281 atccatgccc ttttgccagg tgagccgcga gatggcgatg aacagcgggc catcctcgtg
     2341 ctccagcccg aactccgcct ccagcgcccg cttgttcagc acccggcggt tgaaattgcg
     2401 ggccgaaaag ccggctgcca gcgccggatc ggcagccggg ttccattcgc gcgtgtcgat
     2461 cccgttgata aggccgcgca ccgcatcgcc gcggctgagg atcagccctt ccagccccat
     2521 gccgaactgc ggcgagcgga tctccagcgc ataggtgggc gagacggtgg tgatgcgcgc
     2581 ggccgcctcc agccccgcct tcaaaaagcc cacgccgcca tgatattcca ccccatccat
     2641 cgcccaggcc gcctgcggca ggcccagctg cgggaacaca tcgccgccga aatggccctg
     2701 gaaggccatg ttgtggatgg tcatcacgcc gggcggcaca tgggcgccgg ggcgcgccga
     2761 caccggcggg gcaaagcgga gataggccag cgccatcccc gcctgccaat catgcgcatg
     2821 caccacatcg aaggcaaggc cggttaccgc gccgccggcg atatcggccg cggcccggcc
     2881 aaaggcggca aagcgcaggg cattatccgc ccagtcgcgc ccggcggtat cgacataggg
     2941 gccgccatcg cggacaaaca gcgccggggc atcgagcacc agcaaggggc cagccagccc
     3001 ctcttccgcc ggctctgcca gcagcaggcg ggccggtatc ggaggggctg gcgcgctcat
     3061 cagcgcgggc cattcatgca gcacccgaaa cgccccggcc gacagccgcg ccatcacctg
     3121 cggataaccc ggcaacaggg tgctcatcgc cacgccatgg ggcgccaccg ccgccggcag
     3181 ggcgcctgcc acatcggcca gccccccggt cttgatgaaa ggcaccgcct cggaggccac
     3241 cgacagaacc ctgagcacca cgcgctctcc ctatttcagc cggtcgatca tcggcttggt
     3301 gatgaggcat acgccatttt cggtgcggcg gaagcgtttt gcgtccagct cgggatcttc
     3361 gcccaccacc agcccttcgg gaatgcgcac gcccgaatcg acgatcaccc gcgacaggcg
     3421 cgccccccgc ccgatgttgc aatagggcag gatcacgccc tcctgcacgc tcgaccagct
     3481 gcccatcttc acgcccgtga acagcagcga gcggcgcgcc accgcgcccg agacaatgca
     3541 atcctggctg atgagggaag agatcgccat gccccggcgc ccctcctcgt cgtggacgaa
     3601 cttggccggg ctcgccacca cctgatcggt ccagatgcgc cattcgcggt catacatgtt
     3661 gagcgcgggc acaacatcga tgagatcgag attggcctcg aaataggcat ccagcgtgcc
     3721 cacatcgcgc cagtattcct cgctttcctc ggccgcgcgc acgcagcttt cggaaaagcg
     3781 atgggccacc gccttgccgt gcttgacgat gcgcgggatg atgtccttgc cgaaatcgcg
     3841 gctggaggca ggatcggccg catcctcgcg caggcgctcg aagaggaatt gcgtttcgaa
     3901 cacatagatg cccatgctgg ccaggcaaaa gccgggctgg ccggggattt cgggcggatg
     3961 tgccggcttt tccacgaagt cggtgatcac gctgttggta tccaccgcca tcacgccaaa
     4021 gccgctggca tcctttttgg gcaccaccag gcagccgatc gtcacatccg cgccgctgtt
     4081 cacatgctgg cgcagcatgt gctcgtaatc catcttgtag atatggtcgc ccgccaggat
     4141 gaccatatag cgcacgccat gcgcctcgat gatgtcgatg ttctggtaga cggcatcggc
     4201 ggtgccctca taccattgcg tttccgacac acgctggctg gcgggcagga tgtcgaagct
     4261 ctcgttgcgt tcaggccgca ggaagttcca cgcccggttc atgtggcgga tcaggctgtg
     4321 ggccttgtat tgggtggcaa cgccaatgcg gcggatgccc gaattgatcg cattcgacag
     4381 ggcaaaatcg atgatccggc tcatgccgcc aaaatagacg gccggcttgg cgcgattgtc
     4441 ggtcagctcc ttcaggcggc tgcccctgcc gcccgccaac acataggcca tcgcttctcg
     4501 tgccagcggt gacccgaacg aatccctcat ccgctactcc tttgcccttc agcccgcata
     4561 ttccagcatg atcgtggcca gcggcggcag ggtgacatgc gcccccccgt ccgttgccca
     4621 cacctctccg ccattgccca tgccagagcc gccataggcc gtggcatcgg tgttgacgat
     4681 ttccacccag cgcccatcgg ccggcagcgg caccctgtag ccggtgcgcg gcgccggcgt
     4741 gcaattggcg atcaccgcca ccggccgcgc gcccggcgcc cgccgcgccc aggcaaacac
     4801 gctgttgtgc cgatcatcga cgatcagcca ggcaaacccc tcgccctcgc aatcgcgcgc
     4861 atggagcgcc ggcgtctcgc gatagatccg gttgagatcg cgcaccagca tgcgcacccc
     4921 ctcatgggca ggggctgcca gcaggtccca atccagcccg cgcgcctcgc tccattcatt
     4981 gcgctgggca aattcctggc ccatgaacaa cagcttcttg cccggatagc cccacatcag
     5041 cccatagagc gcgcgcaggc tggcaaattg ctgccaatca tcgccggcca tcttggccag
     5101 caggctgccc ttgccatgca ccacctcgtc atggctgagc gccagcacga aattctcggc
     5161 aaaggcatac atgaggccaa aggtgacatc ctcgtgatgc caggccctgt gcaccggatc
     5221 gcgcccgaaa tagcgcagca catcgtgcat gaagcccatg ttccacttga agccgaagcc
     5281 cagcgctgcc gggcggccgg gggcttcata ggcgggctgg gtaacgccgg gccaggcggt
     5341 cgattcctcg gcgatggtga ggatgccggg ctgggctgca tgcaccgcgc ggttcacgct
     5401 gcgcaggaaa tccaccgctt cccaattctc gcgcccgccg ctttcgttgg gcacccattc
     5461 gccgttctcg cgcgaatagt cgcggtagag catcgaggcc accgcatcga cgcgcaagcc
     5521 atcgacatga tagcgctcgg cccagaacag cgcgttgttg acgaggaagc tggccacttc
     5581 gcgccggccg aaattgtaga tcgcggtgtg ccaatccggg tggaagccct ggcgcggatc
     5641 ctcatgctca taaagagcgg tgccatcgaa gcgggccagg ccatgggcat cggtggggaa
     5701 atgcgccggc acccaatcga ggatcacccc gatcccggcc cggtgcgcgc catcgacaaa
     5761 gcgggcaaag ccctccggcg ggccgaagcg ggcgctgggg gcataaaggc cggtggtctg
     5821 atagccccag ctgggatcga agggatgctc ggacacgggc aggaactcga tatgggtgaa
     5881 gcccatatcg gcgacatagg ggatcagccg ctcggccaat ttgtcccagt tcaagaacca
     5941 gccattttca tcgcgttgcc agctgccggc atgcacttca tagatgctga ccggcacccg
     6001 ccgcggatcg accgaggccc agtgggcgcg gtgcccggca tcgccccaat cctgcttcac
     6061 cgggtgcgcg gtgaggctgg cggtagccgg gcgcagctcg ctggccaggg caaacgggtc
     6121 tgccttcagc ggcagcagcg tgccatcggc gccaaggatg cggtatttat aggcccgccc
     6181 ggcgccgata tcaggcagga agatctccca cacgccaatg tctgcccggc ggcgcatggg
     6241 atggcggcag ggatcccagt cgttgaaatc acccaccagg ctgaccagcc gcgcattggg
     6301 cgcccagacg gcgaaatgca cgccctgcgc cccctcatgg gtgatgagat gcgcgcccag
     6361 cttgtcgaac aggcgcagat ggctgccctc ggcgatcagg taatcgtcga gcgggcccag
     6421 caccgggccg aaggaatagg ggtctgtcac ccaccattgc gtgcccttcg ccccggcgcg
     6481 atatttcacc ggccccggcg gcccggcgat cgccccttca aacaggcccc gcccatccgt
     6541 cagcgccagc gcgcccagcg gctcgcccgc cagcgagaag gcctcggcca cctcggcgcc
     6601 gggcagcagc acgcgggcaa atgtgccatg gggcccggca tggcacccca gaagggcgaa
     6661 aggatcggcg tgggtgcctt ccagcagggc ggtaatggcc tcttcaggtg gtttcacgaa
     6721 acgccccaga tatcctttgc atattcgcca atggtgcgat cggaggagaa ccagccgaca
     6781 ttggcgatgt tgcgaatggc cgccgaacgc cagctggcct gatcttccca gcgcgcatcc
     6841 accgcgcgct gcgtggcggc ataggcttcg aaatcggccg ccaccatgaa ccagtcgtga
     6901 tcgtaaaggc cgcccatcag gtcgcggtag cgatcgggat cgtcggggct gaacacgccc
     6961 gaggcaatcg cgttcaccgc ctggcgcagc tcgcggttct gctcgatcag cgcgcgcggg
     7021 tgatagccat cggcgcgctt ggcggccact tcatcggcgg tgaggccgaa gatgaagatg
     7081 ttctcgtcgc ccacgcaatc cttgatctcg atattggcgc catccagcgt gccgatggtg
     7141 agcgcgccat tgagcgcgaa tttcatgttg ccagtgcccg aggcttccat gccggcggtg
     7201 gaaatctgct ccgacaggtc ggccgccggg atgatccgct cggccaggct gacattgtag
     7261 ttcggcacga agaccacctt cagcagcccg cccaccgacg gatcggaatt gaccctgcgg
     7321 gcgatatcat tcgccagttt gatcaccagc ttggcgttgt gatagctggc cgccgccttg
     7381 ccgccgatga tcttgacgcg cggcacccag tccttctcgg gatggctgcg gatctggtcg
     7441 tagagcgcta ccgtttccac cagattgagc agctggcgct tgtattcgtg gatgcgcttg
     7501 atctgcacgt cgaacagcgc atcggggttc agccgcacgc ccatcagcgc cttcaggtgg
     7561 ttggacagcg ccaccttgtt ggcccgcttc acctcggcga tgcgctcgcc aaaatcgctg
     7621 tcctcggcca gcgcattgag gtcggcaagg cgcgtggtat cgtcgaggaa ggcctcgcct
     7681 atgccatcgc ggatcagcgc ggtgaggccc gggttgcact gctgcagcca gcggcgcggg
     7741 gtgacgccat tggtcttgtt gttgatgcgg gcgggataga gcctgtgcag atcggcaaag
     7801 acggtggatt tcatcagctc tgtgtgcagc gctgccacgc cgttcaccga atgggcgccc
     7861 acaaaggcca ggttggccat gcgcacgcgc cgctcgccgc cctcgtcgat caggctgatg
     7921 gcggcgatct gggcgccatc catgccggcc ttgcgcgcct cgcgcagcac ccggctgttg
     7981 atcgcataga cgatctgcat gtggcgcggc agcaaccgct cgaacaaggg cagcggccag
     8041 ctttccagcg cttcgggcag cagcgtgtgg ttggtgtagg cgatggtctg cttggtgatc
     8101 tcccaggcct cgtcgaattc cagcccgtgc acatcgatca acaggcgcat cagctcggcc
     8161 accgccaccg aggggtgggt gtcgttcagc tggatggcgg ccttttccgg cagcgagcgc
     8221 acatcgccat gatattgcac atggcggcgc aagatgtcct ggatggaggc ggaagtgaag
     8281 aaatactcct gcctgaggcg cagttcctgg ccggccggat tggaatcggc cggatagagc
     8341 acgcgcacca ggctggtggc cttcacctgc tcggccatgg cgccgaaatg atcgcccgcg
     8401 ttgaaggcat cgagccgcag cggatcgagc gcccaggccg tccacaggcg caaggtgttc
     8461 acccgcttgc cgcgccagcc caccaccggc gtgtcgatcg cgctggcctc gatctgctca
     8521 tgcggatgcc aggtgacgcc gccggaattg tcttccacca cctcgccgcc aaagccgatg
     8581 cgataggcac tctcgcgccg gtcgaactcc cacgggttgc catgggccag ccaggtctcg
     8641 ggcagttcca cctgccagcc atcgtcgatc cgctggcgga acatgccgtt cacatagcgg
     8701 atgccatagc cataggcggg gatatcgagg ctggccaggc tttccatgaa gcaggcggcc
     8761 aggcggccca ggccgccatt gcccagcgcc gcatccggct ccagctcttc cagctgggcc
     8821 agttcgaagc catgctcgcg cagcgcccct tccatctcgc gcgtgaggcc catgttcgac
     8881 aggcaatcgc gcatcagccg gccgatgaga aattccaggc tgagataata gacccgcttg
     8941 ccgccgcttt cataagtgcg gcgggtggat tccatccagc ggtcgatcac cttgtcgcgc
     9001 agggccaggg cagcggcgga aaaccagtca tgcgccttgg ccgcgcgctc gtccttgccc
     9061 acgcggtggc gcagcacatc aaggatcagc gcacggatgg gcggcgcggc cagctccacc
     9121 agcgcgccgc tggcgcctgg ctcgtcagtg gcgggcccgg ggtgggcggg aaagggattg
     9181 gcttcgctca cgaacagaac tcccttggct gcaaggttgc gcgcacccgg ctgccggacg
     9241 cgcgaccggc atcatcctgt tcctcccgcc gaggcgccgc cttgccgtcc ggccagggcc
     9301 ggaccggcga agcaccccct cgccaattcc tccggaaccg aggctaggac cacatcgccc
     9361 ggcccgcaag cgcgcaatcg gccgccccgt tcttttccgc aaaaagccgg cccgcccgct
     9421 acagccgctc tgcctgggcg accgcctcgc tcgcccgcaa ggcactgaca aggcgcgaga
     9481 catgcgccag atcatgcacc tccagatcca cctcataggt gccgaaaagc tcgtcgcgcc
     9541 cggccatctt caggctgacg atattggcat ggttgcgcgc gaaaatgccg gtcacctcgg
     9601 ccagcgtgcc gggccggttg tagagcagca cgcgcagccg gctgaccgcc cccttgctcg
     9661 cctcgcccca cgacaggtcg agccattcgc ggtcctcgcc gccggccagc gtctcgcagg
     9721 aaatggcatg cacttccacg cccacgcccg gcttgcgcag ccccacgatg cggtcgcccg
     9781 gcaccgggtg gcagcattcg gccaactgga aggcgatgcc gggcgtgagg ccgcggatcg
     9841 agatagtgcg atcgtggcgc ggccagtgat cggccggcgg cagggcgctg gtgctgcccg
     9901 gcaccagcgc ttccatcacc tgccggtcgt ccagcttggc ggtgccgatc gccgccatca
     9961 gctcgtcctc gctcttcagg ttcagccgct tcagcgcatc gccgatcgcc ttcttgccca
    10021 ccttggtggg cagatgcgcg gcgatatcat caaacagctt gcggccgatg gcggcgatct
    10081 cggcgcgttc cttggcgcgc accgcccggc ggatggccgc ccgcgccttg ccggtgatgg
    10141 caaagcccag ccacgacagc tgcggcgagg cactgcggct cttgatgatc tccaccacat
    10201 cgccattgcc cagcggcgtg cgcagcggca tgtgccgccc gttgatcttg gcgcccaccg
    10261 ccatgctgcc aaggtccgta tgcacggcat aggcaaagtc gatcggcgtt gcccccttgg
    10321 gcagctggaa cagcgccccc ttgggcgtga aggcgaagat gcgatcctga tagatcgcca
    10381 tcttggtgtt ttccagcagc tcttcagggt cgtggctgga atcgagcact tcgagcaggt
    10441 cgcgcagcca gcccacctgg ccatcgggct tgatgccgcc ctgcttgtag ctccagtggg
    10501 cggccaggcc atattcgttg gtctcgtgca tgtcccaggt gcggatctgc acttccatgc
    10561 gcatcgcctt ctcatagatc agcgaggtgt ggagcgactt gtagccattg gccttggggg
    10621 tggagatata atccttgaag cggccgggaa tggcctgcca ggcggtgtgc agcgtgccga
    10681 tcgcgcgata gcaatcctcc acattgtcca ccagcacgcg gaaggccatg atatcggtga
    10741 tcgcctcgaa attgacgtgg cgctcggcca tcttgcgcca gatcgaatag gggtgcttct
    10801 cgcgccccca caccttgcac ctgaggccgg cgttggtcag cgtctggctg atggcgaggg
    10861 caatcgcatc cacctgcccg ccatcgctct cgcggatctg ggcaaggcgg ccggtgatcg
    10921 tcgcatagcc ttccggctcc agctgctgga aagccagcag ctgcatctcg cgcatatatt
    10981 catacatgcc cacccgctct gccagcgggg catagatatc catggtctcg cgggcgatgc
    11041 ggcggcgctt gtcctcgctc ttgatgaaat gcagcgtgcg catgttgtgc agccggtcgg
    11101 ccagcttgac cagcagcacg cgcagatcct cgctcatcgc cagcaggaac ttgcgcaggt
    11161 tttccgccgc gcgctccttc tcgctcatcg cctcgatctt ggagagcttg gtgacgccat
    11221 cgaccatgcg cgccacatcg gggccaaaga gcttctcgat ctcctcggtg gtggccagcg
    11281 tgtcttccac cgtatcgtgc agcagcgcgg tgatgatcgt gtcctggtcg agcttcagct
    11341 cggtcatcag gccggccact tccaccggat ggctgaaata aggatcgcca ctggcgcgtt
    11401 tttgcgtgcc atgcttctgc acggtataga cataggcgcg gttgagcatc gcttcatccg
    11461 cgtcgggtgc ataggccagc accctttcaa caagttcgta ttggcgcaac atcgtgtcgg
    11521 cactgtgggc gataggccgc ccccagggca agggggaaag ggcgcaaaaa ggccgatcag
    11581 cccgatcggt gctgccgatt gccgcctagg aggcctgcgg gccccgccat tgccggcgtt
    11641 cctctgccgc gcgcagcact tcataggcca gctgcagcgc ctggaaggcc ttggccgcct
    11701 cggcatcatt ggggcgcaca tcggggtgca cttccttggc gcggctgcgc caggccttct
    11761 tcaccgcatc gaaatcggca tcattgtcca gccccagcac ttcaagcgcg cgcatctcgt
    11821 cgcggctgcg ggtgccatcg ccgctgccgg cccattcgta ataggccgat tcgcgatagc
    11881 cggcgctggt ctgctcctcg gccttgcggc ggctggcggc ttcctcggca tcgaggccgg
    11941 cgaaataatc ccagccggcg ttgtattcgg ccgcatgggc ctggcagaaa taccagcgtt
    12001 ccgggctgtt gggcgatttg ggcgcggggc aattgccggg ttccgtgcag ccggccctgt
    12061 cgcacaggcg caccttggcc gcctcgcgcg cgctctcata gccgcgccag cgtggaaacc
    12121 cccagtcact tgatcgtctc gcccggctca tggccgggat gattggcgcg gaatggccgg
    12181 ccatgcaagc cttgcgatgc acgcctgcac gcaccccggc ggccatgccg ccggcatgca
    12241 aaaagcccgc caccttgcgg cagcgggcct ttcgtggcgc agccggccgg ccgatcagtc
    12301 gatcgtcacg gtcaccgccc ggcggttctt cgcccaggct tcctcggtgg aagccggatc
    12361 caccgggcgt tccttgccat aggaaatcgt cgtcaggcgg ctggcatcaa cgcccaggct
    12421 gacgagatag ttcttggcgg cagtggcccg gcgctcaccc agggcgatgt tgtattcgcg
    12481 ggtgccgcgc tcgtcggcat ggccctcgat ggtggccttc ttcgccggat acttgagcag
    12541 ccaggcggcc tgcgtcttca ggatctccac cgagagatca tcgacattat acttgtcgaa
    12601 ggcgaagtgg atggtgtcgc gcccgccgat ggaggcgagg aaatcggcct ggctgccggg
    12661 cgtggggccg ctgggcacag gggccggggc gggcgctgcg gccacggtcg ggcccggctc
    12721 gggcggcagc tgcttgggcg gcttgggcgc gcaggcggcc agcgccgtgg cgctgacagc
    12781 caggaggatc agggtacggt tacgcggaaa acgggagagc atggctaact cctttgttgc
    12841 tggagagagg gggatgcgat cctctccgga tcgtttgtta tacacgaggg taaaacaaga
    12901 cgttacggcc gcaccggccc ccaggagggg tcggatgcgt cgacaggggt gggcaggcgg
    12961 cgctcgttgc gcccggtcag gtccacctgc cagatgctgg tgtggcccgc acccttctcg
    13021 gtgcggaaga actggatgat gcggccgttg ggcgcccagg tgggggcctc atcctgccag
    13081 ctgtcggtca gatagcgcag gccctgcccg cccgggctgg tgaccgcgat gcgcaggttg
    13141 ccgccaatgt gggtgaaggc gatctggtcg ccgcgcgggc tccattccgg cgttgccgat
    13201 ttgccgccga agaagctgat gcggtgctgg tcggtgccgt cggcgttcat cacatagatc
    13261 tgctggctgc ccgaacggtc gctctcgaac accaccttgc cgccatcggg cgagaagctg
    13321 ccgccgacat tgatgccggg cgtgtcggtc agctgctggc tgcggccgcc ttcggcaggc
    13381 acgcgataga tgttggtgtt gccggcaatc gacatcgaat agaggatcca tttgccatcg
    13441 ggcgaccagc gcggggcgat caccggattg gtggtggaaa ccaccagccg ctgggtgttc
    13501 tgcgccacat cgtagacata gatgcgcggc gccccgttga gatagctcaa atagacgatc
    13561 ttcttgtaat cgggcgagaa gcgcggcgtc agcgccatcg cctggccgct ggtgatgaag
    13621 cggtggttgg cgccatcgga atccatgatg gccagccgct tcatgcggtg ttccttgggg
    13681 ccggtctcgg cgatataggc gatgcggctg tcgaagaagg ggctttcgcc ggaaagccgc
    13741 gcatagatcg catcggcgca tttgtgggcc gcgcggcgcc agtcggccgc cacgaagttc
    13801 cagatatcgc ccacgcgctg ctgcttcagc gccacgtcat aaaggtagca gcccacctgc
    13861 agctggccat cggccccggc gctcaccgcg ccatgcacca gcatttcggc gccgcgcgcg
    13921 ctccaggcgg caaaatcggg ggcctgcacc tgctccatcg gcacgccggg caattgcccc
    13981 gggcccaccg gcttgaacag gccattgttc ttgagatcgg cggtgatcac ctcggcgatc
    14041 gcccggccca gctgggcagt gctgccggcg ctggcggcgg tgggcacatc ggccggcgtg
    14101 gcaaaggcgg ggatgtcgat gcccaggctc tgccaggcgc tgtcgtccga cacggtgaca
    14161 gagaggccct gatcgcccgc cgccggggcg ggagctggcg gggccgcagc aggcgcgggc
    14221 ggcagatcct gcgcgccggc cggcaccggg gccagcaggg gagccagcag caacatggcc
    14281 gcagccaggg gagaggtcag cctgtcaaat ttccacgtca ttgcgacaaa ttcctgtcga
    14341 accgcacggc ggtcagatgt ttccacgtat cgtaatattc cggaggcagc tgaaacggaa
    14401 ccgccagttg caccgcgcgg atagcattat cgcgatggag cccggcctgc ggccgattgc
    14461 tgtcggtgat gccttcctgg cgcaccacgc gcacggggcc ggccagcgtg ccatcgcggt
    14521 tcagatcgaa ggccagcacg gtgacgagct ggtctgtatc cacgccctgc ggcacgcgcc
    14581 agtgctgctt cagctggcgg gcaatggcgc cgccgatcgc cgcgcgcacg gcagggccgg
    14641 ccgcctgcgc cggggcgccc tggccgcggg tgctgctctg gctgctggtc acgcctttca
    14701 ggaaatcggc gccgatgcgg ctgccgccca ccgggcgatt ggccttgtgg ggctcttctg
    14761 cggttttggc gaaggcatcg cggaaggcat cgggccgggg ccgggcctca gcccggggcg
    14821 ccggctgggg tttgggctga ggctggggat gcggctgggg ccggggcacg ggcgccggcc
    14881 tgggctgggg ttgaggcacc ggctgcggtt tgggctgggg ttggggttcc ggcgcaggct
    14941 ggggcggcgg cgtggtctcg cccagcgtgg gggccagatc ctcggccgcg ggctgctccg
    15001 ggtggggcga ggtggacacc ggccccacat catcggccag cgttaccgcc atgcgctgag
    15061 gggtgggcgg cgccttgcgc gcgggcgcca gcaccagtgc cgccagcagc gccacatgcg
    15121 ccaccaccgc cactgtgagc gcaatccgct catccccgcg cgagagaagt ctggccatcg
    15181 gttcgtcgct aaattgtatc agcttaacgc gcgctgaccg gccgggcgcc gaccggtcgg
    15241 gcctggcccg ccccgccgcc cgcggccacc gttaccagcg agatctggct gatgccggca
    15301 gcgttcagct cgcccatcag cgagaccacc tggccataat ccagcgcctt gtcggcccgg
    15361 agcgtgacca gcggggcctg cccctgcgca tcgcgcggca gattcgccag ccggtcttcc
    15421 agctcgcccg gcgccagcgg cgcatcgtcg aggaagacat tgccatcgcg cgtcatggtg
    15481 atggtgatct ccttgggctg gtgatccagc gagtgggcct tgctgtcggg caggctgatc
    15541 gggatgccgg ccttcagcag cggcgctgtc accatgaaga tgatcagcag caccagcatc
    15601 acatcgacca gcggcgtgac gttgatctcg gccatcggcg cgcggcggct gccccgcccg
    15661 cgctggccct ggcgatgcag ccccatcgcc atcagagctg atccagctcg cggctgaggc
    15721 tggtgtggaa cttgtcggca aagcgctgga gggcggcctc gaacaggttc acgccggccg
    15781 agaagcggtt gtaggcgatc accgccggaa tggcggcgaa gaggcccgtg gccgtggcga
    15841 acagcgcttc ggaaatgccc ggcgccacta ccgccagcga ggaattctgc gcctggccga
    15901 tctggaagaa gctgttcatg atgccccaca ccgtgccgaa caggccgaca aacggcgcca
    15961 cgctgcccac tgtggccaga aaggtgaggc ggccggccag ctcgtcggct tcctgcgcca
    16021 ccaccacgcc catggccgtg gccaggcgct ggcgggcgcc ttccctgtcc accttgccat
    16081 tggccgagcg gcgccattca cccagcgccg ccgacaccac cttgccgatc ggcagatcgc
    16141 gcccgccgct cttttcggcc tggaaccggt cgatatcctg cgcctgccag aaatcgcgct
    16201 cataggcggc cgtgcggcgg cgcagcgcgc ccatccgcca gctgaaggag aagatgatcg
    16261 tccacacgcc gatgctggcc agcaccagcc ccgccatcac cacctgcacc acgatatcgg
    16321 catcgcgaaa cagcttgatc ggatcgagat gcgccggcgc ggcgctgccg gcggccaaaa
    16381 ggagaagatc ggtcattgcg gccctgttgt ggaggaagaa gaggaggaga ggagagacga
    16441 gaaggcagcg cgccagccat cgggttgccg gcgggcgcgc ccatccggcc cgacaaagcc
    16501 gatgcgcacg cgcgcgtcag ccaatagggt gccgttcctg aacgccagct gatgcatccg
    16561 gcagctggcc gcgcgcatgt ccaccacccg gctttcgatc agcacggcat catccagccg
    16621 cgccggcgcc agatagcgca gatgcatctc tgccaccgca taggccccct cgcccgcctg
    16681 ccaggcggcg cgctggtcga tctccagcag gcgcagcatg tcggaacggg cgcgctcgaa
    16741 ccagcgcaga taattggcgt gatagaccac gcccgacagg tccgtgtcct cgaaataggc
    16801 gcgcacggca tagcggtgga ccggcccgtc gaacaggccg gcagggggat cgggaaacat
    16861 gctcatcggc gccagactag ccatggcgcc gggcccgcgg caagatccaa gacgcaaaag
    16921 ctcaacgcgc cagcatcggc cccagcggct ggccgccaaa cagatgcaca tgcagatgcg
    16981 gcacttcctg cccgccatcg ccgccgacat tggccagcag ccgatagccc ggccaggcag
    17041 acccttcgcc cgccagcccc agctggcgcg ccacctggcc caccgcgcgc acgaaatggc
    17101 cgatctcttc agccggcgcc cgggcagaaa aatcctccca gctgacatag gccgccttgg
    17161 ggatcaccag cacatgcacc ggcgcctgcg gggcgatatc gtggaaggcc agcgcgtgat
    17221 cgtcctcata gaccttcttg caggggatct cgccgcgcag gatcctggcg aagatattgt
    17281 tcgggtcata aggctggctg gggtcgatcg gcatggtcgg ctccttgggg gggtaaacga
    17341 ggctggcggc aggttcaggc ggaacggctg gccttttcgg caatgccgct ggtgccctcg
    17401 cgctgctcca gcagggcgca cacctcggcc agggggatct gcctggcgcc cagcagcacc
    17461 agcaggtgga agaggagatc ggccgcctcg cccaccagtt cggcgcggtc gtcggcgacg
    17521 gcggcaatgg cggcctccac cgcttcctcg cccactttct gggcgatttt cttcaccccc
    17581 ttggcatgca gccgggccac ataggaggtt tcggggctgg cggcgcggcg ctgggcaatg
    17641 aggccctcca gccgggcgag aatggcaaga tcggtatcgc tcatggccgc ttgcgatgcg
    17701 cccaagcgcc gctgcggtca agcgcgcaag cgctgctgcg actacgcgcg caagcgctag
    17761 tgcgactaag cgcccaggcg ctcctgcaat gaagcgcccg ggcgcccggt cagtgctggc
    17821 gggcaggcag gcccgatgcg cgcagcgcgg catgggcctc ggcgatggtg tgcttgccga
    17881 aatggaagat gctggccgcc agcacggcgc tggcatggcc ctcgatcacg ccggccacca
    17941 gatgatcgag attgccaacg ccgccgctgg cgatcaccgg caccgacacc gcatcggcga
    18001 tcgcccgcgt cagcgccaga tcatagccgg cctgggtgcc atcgccatcc atgctggtaa
    18061 ccagcagctc gcccgcgccc agccgggcca gcttctgcgc atgttccagc gcatctatcc
    18121 cggtgggccg gcggccacca tgggtgaaga tctcccagcg cccctcggcc acccggcggg
    18181 catccacgct ggccacgatg cactggctgc cgaatttctc ggcgatgtcg ctcaccacct
    18241 cgggccggga aacggcggcg gaattgaccg ccaccttgtc ggcgccggcc agcagcaggg
    18301 cgcgggcatc ttcggcgctg cgcacgccgc cgcccaccgt cagcggcatg aagcacacct
    18361 cggcggtgcg ccgcaccaca tccagcagcg tgccgcgccc ttcatggctg gcggaaatgt
    18421 cgaggaagca caattcgtcg gcgccggccg catcataggc acgggcctgc tccaccggat
    18481 cgccggcatc caccagatcg acgaaattga cgcccttcac cacgcggcca tcgcgcacat
    18541 caaggcaggg gatgacgcga atgcgcaccg acatggatca gccttccttt gccgccagcg
    18601 cgatcgccgc ggccagatcc agccgcccct cgaacagcgc gcggccggtg atcaccccct
    18661 cgatcccctc atgcgcgtgg agcgagagca agcggatatc atccagcccc ttcaccccgc
    18721 ccgaggcgat caccgggatc gccaccgcgc gggccagatc cactgtcgcc tcgatattgg
    18781 cgcccttcag caggccatcg cggccaatgt cggtgaacag caggctggca acgccggcat
    18841 cctcgaagcg gcgggccaga tccaccaccg ccacatccga cacatccgcc cagccttctg
    18901 tcgccacaaa gccatcgcgc gcatccaccg ccacgacaat gccgccggga aaggcccggg
    18961 ccatgtcctt cacgaattgc gggtctttca gcgcggcagt gcccatcacc acccggctga
    19021 cgcccagatc gaaccagcct tccaccgccg ctgccgtgcg aatgccgccg cccagctgca
    19081 catggccggg aaattcctga aggatcgctt ccaccgccgc gcggttctcg gcccggccgg
    19141 caaacgagcc atcaaggtcc accacatgga gatactggct gcccgcctgg gcaaacagca
    19201 tcgcctgatg ggcggggttg tcgccataca cggtggcgcg ggccatatcg ccctcggcca
    19261 gacggacaac ctgacccccc ttgaggtcaa tggcaggaaa gacgatcacg gcatccactc
    19321 cagaaaacgg cccagcaggc ccagcccata ggcctggctc ttttcggggt ggaactgcac
    19381 ccccacgata ttgccccggg ccacggccgc caccagcggg gcgccatggg cagtggtggc
    19441 agcgatatgg ccggcctctt ccggcacgaa atgataggaa tggaggaaat aggcctcgcc
    19501 cggcacgatc aggccgccgg cgggcgcgcc ttgcgccacc accacatcgt tccagcccat
    19561 gtgcggcacc ttgatcgccg ggtcttcagg cgcgatcagc cgcacttcgc cggggatcca
    19621 gcccagcccc cgggtgatgc catgttccac cccgcgcgtg gccagcagct gcatgcccac
    19681 gcaaatgccc agaaacggca cgccatcgcc gcgcacccgc gcgtccatcg cttccaccag
    19741 cccggggatc gcccgcagcc cttcggcaca ggcgcgaaaa ctgcccaccc ccggcagcac
    19801 gatgcgatcg gcgctgcgca ccacatcggg atcggcggtg atggcgacat gatccgcccc
    19861 cgccgctgcc agcgcattgg ccaccgaatg gaggttgccc gcgccataat cgatcaacgc
    19921 cagccggtca gccatggagc gagccatcgc ccagcaggcc cttggtggag gggatcgccc
    19981 cgcccttccg cggatccagc tccaccgcgc tgcgcatcgc ccgcgccagc cccttgtaga
    20041 cgccctcgat gatatggtgg ttgttctgcc cgtagagcag ctcaatatgg agggtgatgc
    20101 cggcggcctg ggcaaagctc tggaaccagt gctcgaacag ttcggtgtcc atctcgccca
    20161 gccgcgcctg gctgaagggc accttccaca ccagccaggg gcggccggaa atatccagcg
    20221 ccacgcgggc cagcgcctcg tccatcggtg aatagacctg gccatagcgg ccgatgccgg
    20281 ccttgtcgcc cagcgcctgc accacgcact ggcccagcgc aatggcgcaa tcctccaccg
    20341 tgtggtgctg gtccacatgc aggtcgccct tgatgtgcac cttcatgtcg atcagcgaat
    20401 ggcgcgagaa ctgctcgatc atatggtcga gaaagccgat gccggtggcg acatcatagg
    20461 cccccgtgcc atcgaggttc agctcgacga ggatatcggt ttcgtgggtc ttgcgggcaa
    20521 tacgggctgt gcgcatgggc ctagccctat aggccagggg gcgcgccaat caatcccttc
    20581 gttaactggc ccctcgttaa cccggcccct cgttaacccg gcggggcacg ccataaggcc
    20641 cctacggcga atcccccctt gaccggggcg cgcgccggcg tcacttggaa cacacaatga
    20701 gcaacgacac gcccgatagc ctgatccctt atgacgaaat cgtgcaggat gccctgcgcg
    20761 ccgtggtcgg ccgcgtgctg ggccaggtgg aaaccaccgg cggcagcctg ccgggcaacc
    20821 accatttcta catcaccttc aagacccatg cgccgggcgt ggacattccc gcccatctga
    20881 aggcgcgctt ccccgacgag atgacgatcg tgctccagaa caagttctgg gagctgaaag
    20941 tggcggccga tcatttctcg gtgggcctct cgttcaacca ggtgccggcc aagctggtga
    21001 tcccctttgc cgcgatcacc gcctttgtcg atccggcggt ggattttggc ctgcagttcc
    21061 aggcctcggc cggcgagctg gagccagagc cgcacgaaga ggcggaaaat gattcgcccg
    21121 aggccgatgc cccccggaca gaaccggccg gcgacggctc caatgtcgtc accgtcgatt
    21181 tcggccgcaa gaaatgagcg gcgcggggcg aggcaaggat gcagcgcccc cggcgccaga
    21241 cagcgcccgc ctgctggccg atgtgctgaa ggaacagggc cacaagctga tcgaccgcac
    21301 ggtgggccgg gtgctgcccg gcgccgctgc tgcaacaccc ccgccggaag cgcccaccaa
    21361 gccctcctcg ccggccgaac ggctggcccg ccgcgtggca ggggccgcgc tgatgcgcat
    21421 cgcctcggcc tcggtgcccg gcgcgatact ggtgggcggc ggcctggtgg cggcccatct
    21481 gcaccggcgc cagcgcgccc gccgcgcccg cgaggccgat cagggctgat tggccggcgc
    21541 tgatcttgcg ctttttgcca aggcccggct tgattggcgc gcccggctgg cgcaaaagcg
    21601 cgcatggcac acaccgatcc caccctttcc ggcaaacctg ccgacacgct gcaccggcgc
    21661 ggcctgatgc tgatcctctc cagcccctcg ggcgcgggca agacgacgat cgcccgccgc
    21721 ctgctggacg tggacaccga aatcgcccct tcgatctcgg ccaccacccg ccccccgcgc
    21781 cccggcgaag tggacggcaa ggactattac ttcatcgacc aggccgaatt cgaccgcatg
    21841 gccgaagcca gcgaattcta cgaatgggcc accgttttcg gcaaaagcta tggcaccccc
    21901 aaggcgcata tccgcgaagg gctgaagcag ggccgcgatt tcctttttga catcgactgg
    21961 cagggcaccc agcaattgtc gcagaaggcg ggcacggatg tggtgcgcgt gttcatcctg
    22021 ccgcccagcc ttggcgaact gcgccgccgc ctcaccggcc gcggcaccga cagcgccgag
    22081 gtgatcgccc agcgcatggc ccgcgcccag gccgagatca gccactggga cgcctatgac
    22141 tatgtcgtga tcaacgagga tgtggatgcc tgtttcgagc aggtgcgcca gatcctcgcg
    22201 gccgagcgca tgaagcgcgt gcgccagacc ggcctcatcc ccttcgtgcg cgccttgctg
    22261 cacggcgaag gctgaaggcg gggcgccagc gcacagcacc ctgccaaaac caccacgcaa
    22321 aaaggccggg gcatgccccc cggccttttc gtttgagcaa gccgggctgg cacgcagcca
    22381 gccccggcag gcccgccttg ccgcccggtg gcggcccggc aggctcagtc ctcgtagttg
    22441 ttgcccagcg agcgcagctc ctggaaggtg gtgtcaacag catcgaggcc ggcgccgttg
    22501 cgatcacgct cctcgaggct gacatgcttg cccaggtgca ccttcacacc caccagcacg
    22561 cgcgaggcgt ggtaggggta gttatagcgc cacgtgtaag agccattgct ctccgtatag
    22621 gtgtccgagc tgacgaagcc gtagtagtcg tagcgggcca gcaggctgac gttggagtgg
    22681 cccagctggt gctcggcctt cacgcccacg ttccaggcat gcgagggata ggtttcgata
    22741 tcgctgtagc tgtaatactg ataccagcgg ccaaaatagt ccgaatattc gtagccggcc
    22801 ttggcggtca gcgcgaaatg cgggttgacg tagtaggtca ggcggccggc ggtctggatg
    22861 ccgcggtcct tgtagtactg atagtcggtg tggtgcgccg acacctggcc gctgatggtc
    22921 agcttgccga catagagctg ggcttcgccg ccgaagtagt aggtggcgcc tgcatagccg
    22981 ctgtagttgg tgttggtggt cgaggtgcgc tgggcgaaca cgcccaccag gcccaggctg
    23041 ggcttgcggg tgaacaggtg cacggccaca tcggtggcat ccagcttggt ggcgccgcac
    23101 tcataggcgt aggtgcacca gtggccggtg gggtgggcga tgttgtgatc aaggccgagg
    23161 tcgatctggg cgccgatgct cttggtgatc ggcagggcca cgctgccgtt cagatcgaca
    23221 gacacgccgc cgtaacggta gccattgttc cagctattgg aatagccgcc ggtctcggtg
    23281 taagtgaaag tttcctggcc aacagtgttg tagcccgctt caaccgaaat atcgccgctg
    23341 atgcccgaaa cagcattggc atgcgcagca gcaggaaggc cggcggccag agccaccgac
    23401 gccataatgg cggtcttttt cataaattcc gtcgcccctc tgaatgacga atattgagca
    23461 ttcaggctcg atgtacttta tgcacagagc ccggaaagcg gggctcgcct agaatccaga
    23521 ccgccccgag gcaacagcta actaggtata attgcggcac accccatcga tttgaagtct
    23581 tttcaggccg catgtgccat aaatgccaca tagtgtagta ttttacttac agtgctgcaa
    23641 acagtcgcga ttctgacaaa aagccgccgc ccgcgcgccg caaacgcccc ttcccgccag
    23701 ccccctgtgc ggttagagac agcccatgga acgccccgcc ccaacgcctg ccgccccccg
    23761 cacgatcccg ctttcgctgt ttgccgccag cctgctgtat ggcgggctgt gcgtgctcgc
    23821 aggggtgctg ggcaccaaga tcgccgatct ggggcaatgg ccggtggtgg gccatctggc
    23881 agtggaatcg ggcatattcg ccttcctcac gctggtggtg ctggccagcg ccacggccga
    23941 actgcatggc acggccaccg ccaaccggct ggtgcggctg ggctttgtgc cgctgatcgt
    24001 gtcgatgctg ctgctgatgc tggtgatcca tgtggtgccg ccggccccct tctggcacga
    24061 tcaggaagcg tttgcccgcc tgctgggcca gggcgcgcgc atgcagctgg cagggcttgt
    24121 ctcctatggc acttcgcaga cgctgaacgt ggcggtgttt gcgcgcctgg ccgggcgcgg
    24181 cggccatggc agcgcgctgt ggctgcgggc gtggatcgcc tcgctgctca gccaggcggt
    24241 ggatacgatg ctgttcatca ccatcagctt tgccggcgtg catgatgccg ccaccggcca
    24301 gcccattccc atcctcacgc tgatgaccgg gcagatcatc gccaagatgc tgctctccac
    24361 gctgatggtg ccggtgctga tccagctggc ggtgctgctg ggccgcaagc ttgatgcgcc
    24421 ctgcgagaac gtctaaagcg cgcgccatgg cacatccgca agaacccgcc ctctcgctcg
    24481 acagcctgct ggccaaggcc gaaacgctga tcgaggccct gccctatctg aagcgctatg
    24541 aaggccgcac cttcgtggtg aaatatggcg gccatgccat gggcgatccg gcgctggcgc
    24601 aggattttgc cgaggatatc gtgctgctga aggcagtagg catcaatccg gtggtggtgc
    24661 atggcggcgg gccgcagatc ggccgcatgc tgaaggcgct gggcatcgaa tcgcaattcg
    24721 tgaacggcct gcgggtgacc gacaaggcca ccgccgaagt ggccgagatg gtgctgtcgg
    24781 gcgccatcaa caaggaactc gtcagctgga tcgccaaggc cggcggccgg gcggtgggca
    24841 tttccggcaa ggatggcgcg ctggtaaccg cgcgcaaggt gtccgcccag aagctgggca
    24901 aggccgtgcc cgatccggaa agcggcgagg cgatcgtcgt ggatctgggc tatgtgggcg
    24961 agcccgaccg gatcgacacc tcgatgctcg acacgctgtc gggcgccggg ctgatcccgg
    25021 tgatcgcccc catcgcggtg ggcaatgatg gcgagaccta caacatcaac gccgacacca
    25081 tggccggcgg cattgccgcg gcgctgggcg cggcgcgcct gttcctgctg accgacgtgc
    25141 cgggcgtgct cgacaagaac aagaccctgc tgaccgacct gaccccggcc gatatcgcca
    25201 ccctggccga agacggcacg atttcgggcg gcatgatccc caagctggaa acctgcgtgc
    25261 atgcggtgga agcgggctgc gaggcggcgg tggtgctgga tggccgggtg ccccatgcca
    25321 tgctgctgga attcttcacc agccggggcg cgggcacgct gatccgcaag ggttgacggg
    25381 aaaaaggggc cgggcaggcc cccaagcctg ctgatatttc ggataagaga attttatttc
    25441 acgggtgcag gccggccggg cggcaagcct gcggttgccc tgcgccggcc cgcaggctag
    25501 gcaacagcgc gaagacctcc gacagaccgg gcttcccccc ggccagcaaa ggatgcgcct
    25561 gccgtgcttt atacgctgat cgacatactg aactatctga tcgagctgct gcgcggcatt
    25621 gtcatcgtgc aattcgtgct cagcctgctg atcagcttca acgtggtgaa cctgcacaat
    25681 gatctggtgc aggcgatctg gcgcgcggtg aacgtgctgc tggagccttt cctgcgcccc
    25741 ttgcgccgga tcatgcccga tacgcggccg atcgatttct cgccgatggc gctgatcgtg
    25801 cttctctcga tcgtgcagat cgtgctgggc cacctggctt tttccgccct ctgacctccc
    25861 cttttcccga gcaggcttca tgaccgacac acacgttgcc cccaccgccg gcgccctcat
    25921 cgatggcaag gctttcgccg ccggcctgcg cgcccgggtg ggcgatctgg ccgcccgttt
    25981 caccgccgag gccggccgca aggcggggct ggcggtggtg ctggtgggcg cggatccggc
    26041 cagccaggtc tatgtggggg ccaagcacaa ggccaccgtt gccgccggca tggaaagctt
    26101 cgagcaccgc ctgccggccg acaccagcca ggccgatctg ctggcgctga tcgagcagct
    26161 caacaccgat cctgccgtcg atggcattct ggtgcagctg ccgctgcccc gccacctcga
    26221 tgagcagcag gtgatcgcca ccatcagccc cgacaaggat gtcgatggct tccatgtcac
    26281 caatgccggg cggctggcgg tgggccagcc gggctttgtg ccctgcacgc cgctgggctg
    26341 cctgatgctg ctgcaggacc ggctgggcga tctggccggc aaggaagcgc tggtgatcgg
    26401 ccgctcgaac atcgtcggca agccgatggc ccagctgctg ctgcaggccg attgcaccgt
    26461 gaccatcgcc caccgccaca ccgaggcgct ggccgaggtg tgccgccgcg ccgatattct
    26521 ggtagtggcc gtgggccgcg cggaaatggt gaaggccgac tgggtgaagc ccggcgccac
    26581 ggtgatcgat gtcggcatca accgcatcca caccgcagat ggcaagaccc ggctggtggg
    26641 cgatgtcgat tatgccggcg tggcgcaggt ggccggcgcc atcaccccgg tgcccggcgg
    26701 cgtcgggccg atgaccatcg ccgtgctgct gcgcaacacg ctggtggccg ccttccgcaa
    26761 cgccggcctg ccgctggacg aaagcgcgat ctgatgggca gcctcggcgg gcgcgggcag
    26821 cagccggggc gggccccggc agcactgggg ctggcggcac tactgctgct ggcgcccccg
    26881 gcgctggccg ccccgccgct ggcgctgccg ccgcaggaag aaccgcatgc caccggccag
    26941 ctggtggaag ggctggccgc gggcgagccc agcgaggtgt tctcggccga attgcagatg
    27001 aagctgcggg cccgcgccaa gggcgatcgc gatgcgatca agcgctttgc cagcccggag
    27061 gcgcaagtgg cagacgcgcg ccagcccggc cacgccccgc tggccggccg ccagccaccg
    27121 gcgctgagcc agcgctcggc cttcctcgcc tgggtcagct gcgacggcac tgtggcgctg
    27181 acgcaaggcc gcctgccggc cgccggcgtg ggcgaggccc attcccgcag ctatctcacc
    27241 gtgtggcagc gccagcgcaa gggcacgccc tatcgcttcg tgctcgatct ggtgagccag
    27301 cagggccaaa gcccggccga ccccgatttc gccgagggcc atgtcgccca gtgcccgctg
    27361 caccggcggg gcgatgcggg ccctgtcggc aagcccggca agccttccaa ggtgcagccc
    27421 cccgccttca ccgccgacac gcccgagcgc ctgaccggcg aggcgcagga ccacacgctg
    27481 cgctgggaac tgacgacgcc ggccgatggc gcccccttct tcgcgcttta cgagaagatc
    27541 gccagcgccc ccaaggaggc gcccgcctat cgcccggtgc tggcgctggg cagcttgcgg
    27601 gctgccgccc ccctcctgcc gccaacgccc gcccccacgc tttaagccgc cttttcgccg
    27661 gcgggggcag cggctaggct gggggcatgg tggatctctt cgtctcggcc ttcttcaccc
    27721 tgttcgtggt gatcgatccg atcggctgcg cgccgatctt cgccggcctc actgccggca
    27781 cgcctgcggc ccatgcgcgc ggcgtggccc tgcgcgccag cctgatcgcc agccttatcc
    27841 tgctggcctt cgcgctgggg ggcaagccgc tgctggccgc gctcaacatc gggctggatg
    27901 ccttccgcat tgccggcggc atcatgctgt tcctcatcgc gctggagatg gtgttcgaga
    27961 aacgcaccca gaagcgcgag gaacgcgccg aaaaggccct gcacacgccg ccggcagagg
    28021 atatcagcgt gtttcccatg gccatgccga tgatcgccgg gcccggctcc atcgccacgg
    28081 tgatgctgat gatgggccgc gcgcaagggc tggctggcgg cgcggtggtg ctggcggcgc
    28141 tggcctgtgt gctggcgatc acgctggtgg ccctgctggc ggcgcggccg atcatggcgc
    28201 tgctgggcag ccgggtggaa gcggtgatca cccgcgtgct gggcgtgctg ctgggggcgc
    28261 tggcggtgca atatgcgata gacgggctgc gcggggtgtt cggcggctag gggcctttcc
    28321 cgcgccctat tgcagggtgg cctggttgtc gtcgctgtcg cgcttgccgt agaactgcat
    28381 cagctgcacc agcaattcgc agcgctcggc cagtttgggc gcttccagca gcacctgttt
    28441 ggaagcggca tcgaacgggg cgatctgcgc cacgccgttg atcagcgcgg catcgtccag
    28501 ccgctgcacc tgttcccaat cgacgcgata gccatgggca tcggcaaagc gatgcgcctc
    28561 gcgctcgaac agggcgcgct ccaccgcggc cagcgcctcg gcctcgggct cgtcgaacag
    28621 ctcggcctcc acctggcgga agggagtgga gacgaccagc tcgcgcccca cgcgaaagcg
    28681 cgcgaggccc tccagcacga tgttgaagcg cccgtcgggc agcgcctcca catcctcgat
    28741 atggcccagg cagcccacgt gatagagcgg cgccccctcc tcgcccgatt gcggctggat
    28801 catgccgatg cgccggtcac gcgccagcga atccgagatc agcgcccggt agcgcggctc
    28861 gaagatatgc agcggcaggc gcagccccgg atagagcacc gcgccagcca gcggaaagat
    28921 cgagatccgc gccatcgcct gcccgctcag ccgaacagga tggtggaaag cttgcgccgc
    28981 tgggcggcca cccacgggtc ttccaggccg atcacctcga agatctgcag caggcggctg
    29041 cgcgcggcgc cctcgttcca cgcccggtcg gccgcgatca ggcgcagcag ggtggccgcc
    29101 gccgcatcgc gctcgccagc ggcaaaggcg gcattggcat aggcaaagcc ggcttcctga
    29161 tcggcagggg cggcttctgc cgcggccttc agctgggcca gctcgccggc ttcgggcgca
    29221 tccttggcca gcgccagggc ggcgcgcgca cgctcgatcg cgggatcggc gccggcgccc
    29281 tcgggcagtt cggcaagggc ggcctcggcc tcttccagat ggcccatgac gatcagcgcg
    29341 cgaatcagcc cggcatgggc agccggcgat tcgggcgcca tttccaggat ctgggcaaag
    29401 atgctgacgg cccgctcggc atcgccgcca tccagcactt cctcgcccat cgccagcagc
    29461 ggctcgatat cgggagcgcc cgcccccgcc ttcaccggca attgcttgag cagctgatcg
    29521 agcagcccct tcagctgcga ttcgctgcgg gcattggtca ggtctgccac cggctggccc
    29581 tggaacagcg catagaccgt ggggatcgag cgcacctgga actggctggc aatgaacttt
    29641 tcctcgtcca cattgatctt ggccaggatc acgcccttgt cggcataatc ggccgccacc
    29701 ttttccagca ccggggtgag cgccttgcac ggcccgcacc attccgccca gaaatcgagg
    29761 atgaccagcg attccatcga aggctcgacg accgtctggc ggaagcgctc caccgccttg
    29821 cgttcatcga gattgaggcc gagacttgcc accctgttat gctccatacc ttgcctgcca
    29881 gcatgcgctg cgcctgttgc ctatttggac cttgcggcgg atttgtgaag aggcgttgcg
    29941 caaaggggct gggggggcgc cggcatcagc cgcttgggca ggcaggcgga aatggcgctt
    30001 ttccgcaagc tgcacgggct atcgcaaggt ttatctgcat ttgcggcgat ttccctcttg
    30061 gcaaggtgga taagggcagc taaaggcccg cctcaccccc gcagcggcgc ccacaagcgc
    30121 cactgccgaa acgccagagc gggcgtagct caggggtaga gcacaacctt gccaaggttg
    30181 gggtcgaggg ttcgaatccc ttcgcccgct ccacgtttca cccggccatg cacccgcatg
    30241 gccaagcccg agcgggcgta gctcaggggt agagcacaac cttgccaagg ttggggtcga
    30301 gggttcgaat cccttcgccc gctccagttt ttccctttta tcacaagata cgcggcagca
    30361 agcatgctgc ccggcgctta gcgcagggca atcgcattcg ccggggagcc gacaccgccg
    30421 accagcggca tgggcgacac cgtgagcagg aagtcataac gacgatccct ggcgcaggcc
    30481 tcggcaagag catcaacatc ccagagttca ccgatgggca ggcccagcag cggcaggatc
    30541 gcccgatgca tgatcccggc atgggcgcca tgaacgccgg tctcgcgctc ttcgcgtgtg
    30601 aaaaacgggc tgtcaggcac ggccggccag cattccaggg caaaattatc cgaggcgatc
    30661 aagctgatgc gcttgtccca aagccaggcc acggtgtcgt gcgactgaag caggccggga
    30721 tggaactggt tctgcacgag gttggcgcgg atctcctccg gggcctggtg ccgataatag
    30781 tcgagccatc cgaagcgaag gatcacgata tcgcccgggc agatctcggc gccctgcgcc
    30841 gcgcaggcct catcgacgag cgcaaccgga atggcctgcc ccttcaggtg atcgatcggg
    30901 gcccccagaa cctctgcgca gtaacgggcc agatcgatca gcacaccgcg cccggcaatg
    30961 ggcgcggcgg cgaagtgatg gatcccgagc aattcggtgc ccggcttgaa ttcggccgga
    31021 tcatagccgt tgtagaaacc gtgatcagga tggccgatat ggcgcaggcc gtcgatctgc
    31081 gagccatatt gggtgtaaag cttgtcgatc cattcatcgt gatgaaaatc gttgcgctgg
    31141 aagatgtgat gctcgagcgg ctggcgggtg ggtgccagtg acgggctgat tgcatcagag
    31201 cgcaggtcga gcgaaaaggc ttcgcccgac cgaaccgcca agaccccgcc aagaacgctg
    31261 tcgcgagaca ggaagttaag cgtgcccagc tgatctgccg gaccgaaaag gccccaggat
    31321 gacccggccg gcgcatcggc gcgggcaagc agttgcgcgt aggatggcat ggccaggatt
    31381 tcgtctcgaa gcatggctgt ttcccgattg ggcgcgatgg cagaccccca tcgggcatgc
    31441 ttcgatcgcg caaagactag cgcgaagaag gccggcccaa tccttcgctg atgcgctggg
    31501 cggcgctgac gagccgtgga accacctgct ccaccatata ggccatcgtt gcttccagcg
    31561 tggagcatga caacgacagc gcggcaacga tatggccgtc ggcatcgcgg attggcgcgg
    31621 cggccgagcg ccgttcgatt tcaagctcct gatcgacggt tgcccatccc tgctccctca
    31681 cctgtcgcag ccgggcgcgc agcgccgcag gatcggcaat cgtcgcaaag gtgagaggtt
    31741 cgagccgtgc gtgcccgagg tagtcctcaa ggcgctcgtc gttgagacca gccagaagca
    31801 ccctgccgct tgccgtcaca taggccggca ggcgcgcgcc cagcggcagg ctgatgctgc
    31861 tgatccggtg gcgatggaca cggctcatgt aaaccacatc atcgccgtcc agcgcgacaa
    31921 gcgccgtgct ggccttcagc tcttcggtca gttcttccat cagcggctgg gcgatcgcgg
    31981 gcaggtcgac cgaggacaga taggcgtagc ccaggtcaag catcctggcc gtcagggcaa
    32041 acttcgcatc cgcagccgta acgaagccca gttgcttcaa ggtcagcagc atccggcgca
    32101 cagtcggctt ggaaaggccc gtcttggcgg ccagctcggg caggccgagg aagcggggcc
    32161 catccgcaaa ggcctgcatg acacgcaggc accgttccac tgactggaca tagtcgcgat
    32221 ctttcccgtc atccatcttg acgcaatgcc tccttacgca taaccgaata cgtacgcaat
    32281 acggattatc cgtacgaatt gcgtacaaca accagaacag agtggcaagc cggtttcagg
    32341 cgtctccctg cgatcggcgc aaggctgcaa cgaacatgat ggagaggata atggaacccg
    32401 atcgcctcaa gctgatcctg cttgatctcg aacagaccct gctcgctttc atccgcaaac
    32461 atggcatcaa ccacgcggaa taccgccgcg ccaccgacct cctgatcgca acgatcaagg
    32521 aaggcgagga atcgctgctg cccgacgtct tcttcgaagc cgagacgacg aacatcggca
    32581 atctgcagcg ggccggcagc ggcgaagcga tcgaggggcc cttctatttt ccgggcgcgc
    32641 cgctgctcac cagcccggca aggctgcccc agcgcgataa cgaggctggc gacatcctgt
    32701 tcttcagcgg ccgggtcacg acgcctgcgg gagatccggt gcctggggcg gagatcgatg
    32761 tctggcaggc cgatgcgcgc gggctttatt cgcaaatcca tcccggcatt ccggactgga
    32821 acctgcgcgg aaggctgcgg accgacgaag agggccgcta cgcgctgcag acgattatgc
    32881 cgccgcccta cgagatcccc aagaacggtc cgaccgggcg cgtgcttgcc gcgctgggcc
    32941 ggcatttctt ccgccctgcc catctgcacg tcaagctgcg ccatgccaac ctgggcgaga
    33001 tgacctccca gctctatttc cacggcgacc catatcttga aagcgatgtc gccaacgcgg
    33061 tgcgcgacga tctcatcgtg gatcttgtgc ggcacgcctc gcaacaggcc ctggcggaca
    33121 ggaagctcga ccagccgttc ttcgaggcct cctacgattt cgtgctaggc tgaccggcgc
    33181 gctcttgccg ccgcatggcc gcccctgcgc cgcatccgcg acggacagcc gccacaggca
    33241 tgaggccagc accaggccac accccccttg ataaccgggt tcagaccatg tccaggatcg
    33301 acagcatcac cacccagcgc ctcgatatcg cgctgcgcca gcctatcacc atgtcgaccg
    33361 gcaccgtctc caggcagaac atcctgttgg tacggctacg cgacaccgat ggcgccgaag
    33421 gtgtcgggga agcgctggtg atgggcggcc cctattgggg gcaggagacg atcgagggcg
    33481 tgagggccgt cgtcgagcgc catctggcac cactgctgat cggccagtcg acatcctcgg
    33541 tcggcgcgat cagccggcaa ctgccgcgat gggtgcgagg gaacgcggcg gcccgttcgg
    33601 cggtggagat ggccatgctc gaccttgtcg gccatcgctc cggcctgccc gtctcggcct
    33661 tgctgggcgg cgcggttcgc agccgcattc ccgtggcctg gacgctttcc accggtagcg
    33721 tggagaccga tattgccgaa ggcgaaaggg ccgctgcggc ccatggcttc acgcgcttca
    33781 aactcaaatt cgggcacgag gcgccccggg ccgacgtttc gcgggcccat gccatcgcaa
    33841 gacatttcgc aggacgcgcg accatcatcg ccgacgtcaa ccagggatgg gatgagcaga
    33901 ccgccattca gatgctgccg cgcctggtcg acgcgggact ggaagccctg gagcaaccgc
    33961 tccccgcgca cgccctggcc gctgcggctg aactgcaacg ccgtttcggg cttcgcttca
    34021 tcgccgatga ggcggtcgat ggtccagcct cggtccttcg actcgcccag gcaaaggcgg
    34081 cggcagccct gtcgctcaag cccaatcgcg acggcgggct gatcgcaacg cgcgatgccg
    34141 ccatcatcgc agccgcggcc gggatacagc tctacggcgg ttcaatgctg gaaacctcgc
    34201 tgggaaccgc ggccaatctg cactgctacg cgaccgcccc cgacctgtcg ctgggctgcg
    34261 agctcttcgg cccgatgcga ctgggcgagg acatcgcaac cactcctgtg tccatcgaac
    34321 aaggccatct cacgctggaa gacacattgc ccggtctcgg cgtcagcctc gatgaagaga
    34381 agatcgcatt cctcgccgcg cgcagtggct gatttggcaa aagaggtgca ggacgcctgc
    34441 atcatgagac aggggcaccc gagggccgga aacaggcttc ccctgagcgc ccccgcacag
    34501 gcgccggctc accccttcat gcgctgcgca tgccattcga catgttctgc catgaaggtg
    34561 gagatgaaat agtagctgtg gtcgtagccc tgttgcaggc ggatggtggc ggggataccg
    34621 gccgcctggc aggccgcttc cagcagaggc gtcttcagct ggccgtccag gaagccatcg
    34681 gccgcgccct ggtccaccag cagatcgggc aggcgggcgc catcggcgat cagcgcgcag
    34741 gcgtcatagg cgcgccag
//
LOCUS       DASRYE010000012         9812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_124183,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000012 DASRYE010000000
VERSION     DASRYE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124183"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..128
                     /locus_tag="VFF98_00505"
     CDS             12..128
                     /locus_tag="VFF98_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62133.1"
                     /translation="MRRSGNAPELRCYSEADDAATAAAINAACLQAVLADFG"
     gene            complement(152..700)
                     /locus_tag="VFF98_00510"
     CDS             complement(152..700)
                     /locus_tag="VFF98_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU62134.1"
                     /translation="MTPPAAPPAPALLIVEDDPAFGRTLRRSFERRGYRVELITQPEE
                     LPGALARGKFDYAVVDLKLGTASGLACVAALHEHDPAICIVVLTGFASIATAVEAIKL
                     GAAHYLAKPANTDDIEDAFHRREGDPDVPVEGRKTSIKTVEWEYIHSTLAECDFNISE
                     AARRLGMHRRTLARKLEKQQVK"
     gene            complement(697..1983)
                     /locus_tag="VFF98_00515"
     CDS             complement(697..1983)
                     /locus_tag="VFF98_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU62135.1"
                     /translation="MSPPPGQQIAPASKRNMVLLVQLRWIAVAGQLLTIWFAWAMLGV
                     DLPLRQLVAVPLLLALVNIVTMVMTDQRRGYSYLELLGALMLDVEALSWQLYYTGGAT
                     NPFTFLFLLQIVIGAILLHPRWSWIVALVACGDVTFLTFRFRPLALPARFGDGHFQLY
                     LYGSLVCFILIAALMVFLVVKMDSNRRASDAALATLRQQASEEHHIIRMGLLATGAAH
                     ELGTPLASMSVILGDWAHLDGLGPDLAEDVAAMQAELARCKSILSGILMAAGEVRGEN
                     PAVTSVRAFLGEIVADWQGRTEAPIRLVDHFGTDVAIVSDPALRQVIGNVVDNALEVS
                     PTGIEIEAWRRGDALLIDIADHGPGFPRAMLENVGRPYASTKGRAGGGLGLFLVVNVL
                     RKLGGRAEVSNLEGGGALVRLIIPLAALAFAGKKRG"
     gene            complement(1997..2722)
                     /locus_tag="VFF98_00520"
     CDS             complement(1997..2722)
                     /locus_tag="VFF98_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZU62136.1"
                     /translation="MSGPARASKGRSRLVPVAALLLCAALFAALGLWQVKRLAWKEAL
                     IAQVSANTTAAPLDVAALPRPLDPAAQEYRRIRLAGRFVPQATVLVAATSDLGNGYWD
                     MVPLAIDGRAPVYINRGFVPLGSRVGQIAAATPTGRVTVVGLLRKSEPHGSFLRPNRP
                     QDDRWYGRDIAAMAAARHIASEPAWFVDAQAENPAPPAAQAPVPGLTVIAFPNNHLSY
                     ALTWFALCALSLGLAVFIGRGRL"
     gene            complement(2739..3119)
                     /gene="cyoD"
                     /locus_tag="VFF98_00525"
     CDS             complement(2739..3119)
                     /gene="cyoD"
                     /locus_tag="VFF98_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HZU62137.1"
                     /translation="MADHSHAHGHDDHGHGAGFHASLKDYVTGFVLSVILTAIPFWLV
                     MSGAIADKGTAALVITGFAAVQVVVHMIYFLHMNAKVEQGWNLMALIFTLLLVAIVIS
                     GTLWVMFHLNANMMPMPMRTDGIG"
     gene            complement(3119..3733)
                     /gene="cyoC"
                     /locus_tag="VFF98_00530"
     CDS             complement(3119..3733)
                     /gene="cyoC"
                     /locus_tag="VFF98_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HZU62138.1"
                     /translation="MSSAELTGQYYDLHEHDHPEGGSTALGFWIYLMSDCLIFAMLFA
                     TFGVLGGNYAAGPGPKDLFDLPLVAVNTAMLLFSSITYGFAMIAMQSGKKNLVLLWLA
                     ITGLFGAAFLGIELTEFHHLIEEGAGPQRSAFLSAFFTLVGTHGLHVTFGSIWLVTLM
                     VQVGRFGLTTANQRRLACLSLFWHFLDVVWIGVFTFVYLMGVLR"
     gene            complement(3730..5745)
                     /gene="cyoB"
                     /locus_tag="VFF98_00535"
     CDS             complement(3730..5745)
                     /gene="cyoB"
                     /locus_tag="VFF98_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HZU62139.1"
                     /translation="MIAHHPATWSPLLGRLGIDAIPHEPIVIGTFFAVALGGAAVLAL
                     LTYFKLWGYLWKEWFTSVDHKKIGIMYMVLGLIMFLRGFADAVMMRGQQAMAFNGSQG
                     FLTSEHFDQVFTAHGVIMIFFVAMPFVTGLMNYVVPLQIGARDVSFPFLNNFSFWMTV
                     GGAVLVMASLFLGEFARTGWLAYPPLSGLAYSPDVGVDYYIWSLQVAGVGTTLSGVNL
                     IATILKLRAPGMSMMKMPVFTWTALVTNVLIVAAFPVLTVVLALLGLDRTLHTNFFTN
                     DFGGSAMLYVNLIWIWGHPEVYILVLPIFGVFSEITATFCGKRLFGYSSMVYATVVIG
                     VLSYIVWLHHFFTMGSGASVNSFFGISTMVISIPTGAKMFNWLFTMYKGRIRYELPMM
                     WTVAFMLTFVIGGMTGVMLAVPPADFVLHNSLFLIAHFHNVIIGGVVFGVFAAINYWW
                     PKAFGFRLNQFWGKVQFWCWLVGFWVAFTPVYILGLMGVTRRMRVFDDPSLQIWYVIA
                     AFGALLIAAGIGAMLLQFGISIWKRDEYKVEGDPWEGRTLEWSTSSPPPEYNFAFTPK
                     IHELDAWSDMKAKGVARPTEGFRAILMPKNTWAGIVIAGMATACGFAMIWQIWWLAAL
                     SLASIVATAIFHTFNYNRTFHIPAEHVAEVEAARTAQLAALSKGGAA"
     gene            complement(5759..6904)
                     /gene="cyoA"
                     /locus_tag="VFF98_00540"
     CDS             complement(5759..6904)
                     /gene="cyoA"
                     /locus_tag="VFF98_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HZU62140.1"
                     /translation="MSTPISTPLRRIRVAGAAAALLMLSACEHANIFEPSGDVAQRQA
                     HLVLLSTGLMLLIIVPVMLLTAFFAWHFRAGNRDASYDPEFHHSTKVELVVWSAPLAI
                     IIALGSVTWVYTHLLDPFRPLDRLAEGQPVTAADKPLEVEVVALDWKWLFIYPEQKVA
                     SVNELALPTGRPVHFRLTSATVMNSFFVPAIAGQIYAMPGMASQLNAVVNKPGTFRGF
                     SANYSGAGFSQMHFNTLGLEPAAFDKWVADTKAAGGNLDAATYLKLAEPTIAEPVHHF
                     GAVSADLFDKVVNLCVEPGKMCVNQMAMLDARGGTGKAGLFNLAQLTYDKDGRPNLTP
                     LTAARQAQLDQRLNKAFVAALCATGPAPLAAKAPKLTLSQPAPSPLS"
     gene            7099..8442
                     /locus_tag="VFF98_00545"
     CDS             7099..8442
                     /locus_tag="VFF98_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62141.1"
                     /translation="MTNAAYDRAGVATATGDASVSPSDIAIGVIIGRTSEFFDFFVYA
                     IASVLVFPKFFFPFLDPLHATLWSFVIFSLAFIARPVGSFVFMAVDRSWGRAAKLTIA
                     LFMLGGSTAAISFLPDYRTAGTSAIALLALFRLGQGFALGGSWDGLASLLALNAPTNQ
                     RGWYAMIPQLGAPLGLVVASSMFAFFISTLPAEDFLAWGWRFPFFVAFAINVVALFAR
                     LRIVESPEFKRLFESRDLQPEPALITLSTQWRHILIGAFAPLASFALFHMVTVFPLSW
                     VYLYTPEKPVSFLVIETGAAIIGIFAIIISGHLADRLGRRMVLGCSAAAIAAFSGFAP
                     QMLGMGTVGEISFMVSGFALLGLSFGQSSGAVAANFPNRSRYTASALTSDIAWLLGAA
                     FAPLVALLLATDFGLIASGLFLLSGALATLVALWANRELGGARHTGDGDMALSAM"
     gene            8664..8855
                     /locus_tag="VFF98_00550"
     CDS             8664..8855
                     /locus_tag="VFF98_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZU62142.1"
                     /translation="MTKLFSRTSIGRLAGNSKGATAIEYGLIAALIAVAAITAMSGLG
                     GQLSSTFNTTSSAMGSAAS"
     gene            complement(8965..9216)
                     /locus_tag="VFF98_00555"
     CDS             complement(8965..9216)
                     /locus_tag="VFF98_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62143.1"
                     /translation="MDGSRYHRNRGCKAHDNAYGLNGGGTGADRLAADLALFRHMRAQ
                     RDPLALVVLGFTRGFGWFFFNYHGLPWSGQLIRRLFPRY"
     gene            9269..>9812
                     /locus_tag="VFF98_00560"
     CDS             9269..>9812
                     /locus_tag="VFF98_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62144.1"
                     /translation="MAGFKRKLFYLGGFDPRGGRYYHALFAQELARAQGPAWLGDRQR
                     RKGGCGWLAGDGAQPACLCDCTFLAWDDLVRRHWPRGLALGWGLWRAYGGFARHLRWG
                     ALHGARPAALIALLWPGLWLLLAPVEIGLAAAALVALGGVALGAAGGLLLALLWGRGL
                     RQAQAPWLARWLIFNDRCARR"
BASE COUNT         1777 a         3128 c         3300 g         1607 t
ORIGIN      
        1 cgatcctgca cctgcgccgc tcgggcaatg cgccggaact gcgctgctat agcgaggcag
       61 atgatgcggc aaccgcagcg gccatcaatg ccgcctgcct gcaagcggtg ctggccgatt
      121 tcggctgaat gaagcggggc aggggggggg ctcacttcac ctgctgcttc tccagcttgc
      181 gggccagcgt gcgccggtgc atccccagcc ggcgcgccgc ctcggaaatg ttgaaatcgc
      241 attcggccag ggtggagtgg atatattccc actccaccgt cttgatcgag gtcttgcgcc
      301 cttccaccgg cacatccgga tcgccctcgc ggcggtggaa ggcgtcttct atgtcgtcgg
      361 tattggcggg cttggcgaga taatgggccg cccccagctt gatcgcttcc acagcggtgg
      421 cgatgctggc aaagccggtg agcacaacga tgcagatcgc cggatcatgt tcgtgcagcg
      481 ctgccacaca ggcaagcccg ctggccgtgc ccagcttcag atccaccacc gcataatcga
      541 atttgccgcg cgccagtgcc cccggcaatt cctccggctg ggtgatcagc tctacccgat
      601 agccccgcct ttcgaacgaa cggcgcaagg tgcgaccgaa agccgggtcg tcctccacaa
      661 tcagcagggc aggggcgggc ggggcagcgg ggggcgtcat ccgcgctttt tgccggcaaa
      721 ggccagcgct gccaagggaa tgatcagccg caccagggcg ccgccgcctt caaggttgct
      781 cacctcggcg cggccgccca gcttgcgcag cacattcacc accagaaaca gccccagccc
      841 gccgccggcc cgccccttgg tgctggcata aggccggccg acattttcca gcatggccct
      901 ggggaacccc ggcccatgat cggcaatgtc gatcagcagg gcatcgcccc ggcgccaggc
      961 ctcgatctcg atcccggtgg gcgagacttc cagcgcattg tccaccacat tgccgatcac
     1021 ctggcgcagc gccggatcgg agacgatcgc cacatcggtg ccgaaatgat cgacgaggcg
     1081 gatcggcgct tcggtgcggc cctgccaatc ggccacgatc tcgccgagga aggcgcgcac
     1141 gctggtaacc gcggggttct cgccgcgcac ctcgccggcg gccatcagta tgccggagag
     1201 gatcgacttg cagcgcgcca gctccgcctg catcgctgcc acatcctcgg ccagatccgg
     1261 gcccagccca tcaagatggg cccagtcgcc cagaatcacc gacatcgagg ccagcggcgt
     1321 gcccagctca tgggcagcgc ccgtggccag caggcccatg cggatgatgt gatgctcctc
     1381 gctggcctgt tggcgcaagg tggcaagggc cgcgtcgctg gcgcgccggt tgctgtccat
     1441 cttcaccacc agaaacacca tcagcgcggc aatcaggatg aagcagacga ggctgccata
     1501 gagatagagc tggaagtgcc cgtcgccaaa gcgggccggc agcgccagcg ggcggaagcg
     1561 gaaggtgagg aaggtgacat cgccgcaagc caccagcgcg acgatccagc tccagcgcgg
     1621 atgcagcagg atggcgccga tgacgatctg cagcaggaac aggaaggtga agggattggt
     1681 ggcgccgccg gtgtagtaaa gctgccagct cagcgcctcc acatccagca tcagcgcgcc
     1741 cagcagttcc agataggaat agccgcgccg ctggtcggtc atcaccatgg tgacgatatt
     1801 gaccagcgcc agcagcagcg gcaccgccac cagctggcgc agcggcagat ccacccccag
     1861 catcgcccag gcaaaccaga tggtgagcag ctggcccgcc accgcgatcc agcgcaattg
     1921 caccagcagc accatgttgc gcttgctggc gggcgcgatc tgctggccag gtggcgggct
     1981 catggtcaaa gggccgtcac agccggccgc gcccgatgaa caccgccagc cccaggctga
     2041 gcgcacacag cgcaaaccag gtgagcgcat agctcaaatg gttgttcgga aaggcgatga
     2101 cggtgaggcc cggcaccggg gcctgggcgg caggcggtgc ggggttctcc gcctgcgcat
     2161 cgacgaacca cgccggttcg ctggcgatat ggcgggcggc ggccatggcg gcaatgtcgc
     2221 ggccatacca gcggtcgtcc tgcgggcggt tggggcgcag gaaactgcca tgcggctcgc
     2281 tcttgcgcag caggcccacc accgtcaccc tgccagtggg cgtggcggcg gcaatctgcc
     2341 ccacccggct gcccagcggc acgaagccgc ggttgatgta gaccggcgcc cggccatcga
     2401 tggccagcgg caccatgtcc caatagccat tgcccagatc gctggtggcg gccaccagca
     2461 cggtggcctg cggcacaaac cggccggcaa ggcggatgcg gcgatattcc tgcgccgccg
     2521 gatcgagcgg gcggggcagg gcggcgacat ccagcggggc ggcggtggta ttggcgctga
     2581 cctgggcgat cagcgcttcc ttccaggcca ggcgcttcac ctgccacagg cccagcgcgg
     2641 cgaacagggc ggcacacagc agaagggcgg ccaccggcac aagccgggac cgccccttgc
     2701 tggcccttgc ggggccgctc atcaggcgag cctgatcatc agccgatgcc atccgtgcgc
     2761 atgggcatgg gcatcatgtt ggcgttgagg tggaacatca cccacagcgt gccggagatg
     2821 acgatggcca ccagcagcag ggtgaagatc agcgccatca ggttccagcc ctgttccacc
     2881 ttggcgttca tgtggaggaa gtagatcatg tgcaccacca cctgcaccgc ggcaaagccg
     2941 gtgatgacaa gggctgcggt gcccttgtcg gcgatggcgc cgctcatcac cagccagaac
     3001 gggatggcgg tgagaatgac cgacagcacg aagccggtga cataatcctt cagcgaggcg
     3061 tggaagcctg cgccatggcc atgatcgtca tggccgtgag cgtgagaatg gtcggccatc
     3121 agcgcagcac tcccatgagg tagacaaagg tgaaaacgcc gatccacacc acatcgagga
     3181 agtgccagaa cagcgagagg caggcaaggc ggcgctggtt ggcggtggtg aggccaaagc
     3241 ggcccacctg caccatcagc gtcaccagcc agatcgagcc gaaggtgacg tgcaggccgt
     3301 gggtgcccac cagcgtgaag aaggccgaca ggaacgcact gcgctgcggg ccggcgccct
     3361 cttcgatcag gtggtggaac tcggtcagct cgatgccgag gaaggcggcg ccaaacaggc
     3421 cggtgatcgc cagccacagc agcaccaggt tcttcttgcc cgactgcatg gcaatcatgg
     3481 caaagccata ggtgatcgag ctgaacagta gcatcgccgt gttcactgcc accagcggca
     3541 ggtcgaacag gtccttgggg ccggggcctg ccgcataatt gccacccagc acgccaaagg
     3601 tggcgaacag catggcaaag atgaggcagt cgctcatcag gtagatccag aaacccaggg
     3661 cggtggagcc gccctcgggg tgatcatgtt cgtgcaggtc gtagtactga cccgtcagtt
     3721 cggcgctgct catgcggccc ctcccttgct gagcgcggcc agctgggccg tgcgggcggc
     3781 ttccacctcg gcgacatgtt cggccgggat gtggaaggtg cggttgtagt tgaaggtgtg
     3841 gaagatcgcc gtggcgacga tcgaggcaag cgacagcgcc gccagccacc agatctgcca
     3901 gatcatcgca aagccgcaag ccgtggccat gccggcgatg acgatgccgg cccaggtgtt
     3961 cttgggcatg aggatggcgc ggaagccctc ggtggggcgg gccacgccct tggccttcat
     4021 gtcgctccag gcgtccagct cgtggatctt gggggtgaag gcaaagttgt actcaggcgg
     4081 cggcgaggag gtcgaccatt ccagcgtgcg gccctcccag ggatcgcctt ccaccttgta
     4141 ttcgtcgcgc ttccagatcg agatgccgaa ctgcagcagc atggcgccga tgccggccgc
     4201 gatcagcagc gcaccaaaag cggcgatgac ataccagatc tgcagcgagg ggtcgtcgaa
     4261 cacgcgcatg cggcgggtca cgcccatcag gccgaggatg tagaccgggg tgaaggccac
     4321 ccagaagccc accagccagc accagaactg caccttgccc cagaactggt tcaggcggaa
     4381 accgaaggcc ttgggccacc agtagttgat ggcggcgaac acgccgaaca ccacgccgcc
     4441 gatgatgacg ttgtggaagt gggcgatgag gaacagcgag ttgtggagca cgaaatcggc
     4501 cgggggaacg gccagcatca cgccggtcat gccgccgatc acgaaggtga gcatgaaggc
     4561 aacggtccac atcatcggca gttcataccg gatgcggccc ttgtacatgg tgaacagcca
     4621 gttgaacatc ttggcgccgg tggggatcga gatcaccatg gtggagatgc cgaagaagct
     4681 gttgaccgag gcgcccgagc ccatggtgaa gaagtggtgc agccacacga tgtagctgag
     4741 cacgccgatc accacggtgg catagaccat cgaggaatag ccgaacaggc gcttgccgca
     4801 gaaggtggcg gtgatttccg agaacacgcc gaagatcggc aggacgagga tgtagacctc
     4861 ggggtggccc cagatccaga tcaggttgac gtacagcatg gcgctgccgc cgaaatcatt
     4921 ggtgaagaag ttggtgtgga gggtacggtc gaggccgagc agggccagca ccacggtcag
     4981 cacggggaag gcggccacga tcagcacgtt ggtgaccagc gccgtccagg tgaagacggg
     5041 catcttcatc atgctcatgc ccggggcacg cagcttgagg atggtggcaa tgaggttgac
     5101 gcccgaaagc gtggtgccca caccggccac ctgcagcgac cagatgtaat aatcgacacc
     5161 cacgtcaggg ctgtaggcca ggcccgaaag gggcggatag gccagccagc cggtgcgcgc
     5221 gaactcgccg aggaacagcg aggccatcac cagcaccgcg ccgcccaccg tcatccagaa
     5281 cgagaagttg ttcaggaagg ggaaggagac gtcgcgcgcg ccgatctgca gcggcaccac
     5341 atagttcatc aggccggtca caaagggcat ggccacgaag aagatcatga tcacgccatg
     5401 cgcggtgaac acctggtcga agtgttcgga tgtgaggaag ccctggctgc cgttgaaggc
     5461 catcgcctgc tggccgcgca tcatcaccgc atcggcaaag ccgcgcagaa acatgatgag
     5521 gcccagcacc atatacatga tgccgatctt cttgtgatcg acgctggtga accactcctt
     5581 ccagagatag ccccacagct tgaaataggt gagcagcgcc agaacggcag cgccgcccag
     5641 agccacggcg aagaaggtgc cgataacaat cggctcatgc gggatggcat cgataccgag
     5701 ccttcccagc aggggcgacc atgtcgccgg atgatgtgct atcatgggtt tgtcgcaatc
     5761 acgaaagagg agaaggggcg ggctgagaaa gcgtcagctt cggggccttg gcggccagcg
     5821 gcgcggggcc ggtggcgcac agggcagcca cgaaggcctt gttcaggcgc tggtccagct
     5881 gggcctggcg ggcggcggtc agcggggtca ggttcgggcg gccgtccttg tcgtaggtca
     5941 gctgggccag gttgaagagg cccgccttgc cggtgccgcc acgggcatcc agcatcgcca
     6001 tctggttgac gcacatcttg cccggctcga cgcacaggtt gacgaccttg tcgaacaggt
     6061 cggccgacac ggcgccaaag tggtgcaccg gctcggcgat ggtgggctcg gccagcttca
     6121 gataggtggc ggcatcaagg ttgccaccgg ctgccttggt gtcggccacc cacttgtcga
     6181 aagcggccgg ctcaagcccc agcgtgttga aatgcatctg cgagaagccc gcgcccgaat
     6241 agttggccga gaaaccgcgg aaagtgccgg gcttgttgac caccgcgttc agctggctgg
     6301 ccatgccggg catcgcatag atctggccgg cgatggccgg cacgaagaag gagttcatca
     6361 ccgttgccga ggtgaggcgg aaatgtaccg gccggccggt gggcagggcc agttcgttaa
     6421 cgcttgccac cttctgctcg ggatagatga acagccattt ccagtcgagc gccacaactt
     6481 ccacttccag cggcttgtcg gcagcggtca caggctggcc ctcggccagg cgatcgagcg
     6541 ggcggaaggg atcgagcagg tgggtgtaaa cccaggtgac cgagcccagc gcaatgatga
     6601 tcgccagcgg cgccgaccac accaccagct ccaccttggt ggaatggtgg aattccggat
     6661 cgtagctggc atcgcggttg ccggcgcgga agtgccaggc gaagaacgcc gtcagcagca
     6721 tcaccggcac gatgatcagc agcatcaggc ccgtcgacag cagcaccaga tgggcctggc
     6781 gctgggcgac gtcgcccgat ggctcgaaga tgttggcatg ctcgcatgcc gaaagcatca
     6841 gcagcgcggc ggcagcaccg gccacgcgga tgcggcgaag gggggtggat atgggcgtgc
     6901 tcatggtgcg cgcgtatgcc caccttttcg caagtgcgat aggacatttt gtctcaccaa
     6961 tttgattgcc atcaacgacg acttcgggca ccatccatcc agcgaccggg taggcagcct
     7021 caaacctgcg gttcgcgctt cccggcgcgg atcgaacacg cacccacaag agggcgtgac
     7081 acatgtgagg atgccaatat gaccaacgcc gcctacgacc gggccggggt tgccaccgca
     7141 accggagacg cttcggtttc gccatccgat atcgccattg gcgtgatcat cggccgcacc
     7201 tcggagtttt ttgatttctt cgtctatgcc attgcttctg ttctggtatt cccgaagttc
     7261 ttcttcccct tcctcgatcc gctgcatgcc acgctatggt ccttcgtgat cttctcgctg
     7321 gcctttatcg cgcggccggt gggctctttc gtgttcatgg cggtggatcg cagctggggc
     7381 cgcgcggcca agctgacgat cgcgctgttc atgctggggg gcagcaccgc ggcgatctcg
     7441 ttcctgcccg attatcgcac cgcgggcacc agcgcgattg cgctgctggc gctgttccgg
     7501 ctggggcagg gctttgcgct ggggggcagc tgggatgggc tggcctcgct gctggcgctg
     7561 aacgcgccaa ccaaccagcg cggctggtat gcgatgatcc cgcagctggg ggcaccgctg
     7621 gggctggtgg tggccagcag catgttcgcc ttcttcatct ccaccctgcc ggcggaagat
     7681 ttcctcgcct ggggctggcg cttcccgttc ttcgttgcct tcgcgatcaa tgtggtggcg
     7741 ctgtttgccc gcctgcgcat cgtggaatcg cctgaattca agcgcctgtt cgaaagtcgc
     7801 gacctccagc cggaaccggc gctgatcacc ctttccaccc agtggcgcca tatcctcatc
     7861 ggcgcctttg ccccgctggc cagctttgcg ctgttccaca tggtcaccgt gttcccgctc
     7921 agctgggtct atctctacac gcccgaaaag ccggtctcct tcctcgtcat cgaaaccggg
     7981 gcggcgatca tcggcatctt tgccatcatc atctcgggcc atctggcaga ccggctgggc
     8041 cggcgcatgg tgctgggctg ctcggccgcg gcgattgccg cgttcagcgg ctttgccccg
     8101 cagatgctgg gcatgggcac ggttggcgag atcagcttca tggtctcggg ctttgcgctg
     8161 ctggggctct ccttcgggca aagttcgggc gcggtggcgg ccaatttccc caaccgcagc
     8221 cgctacaccg cctcggcgtt gacaagcgac atcgcctggc tgctgggggc cgctttcgcg
     8281 ccgctggtgg cgctgctgct ggcgacggat ttcgggctga tcgcctcggg cctgttcctg
     8341 ctgtcgggcg cgctggccac gctggtggcg ctgtgggcca accgtgaact gggcggcgcg
     8401 cgccacaccg gcgatggcga catggccctc tcggccatgt aggccagcta tccgatccgg
     8461 cggtttcccc cgggagggaa cggggcggga ggcgcagggc gcgctttccc gccccgtatt
     8521 tttggggccc gccggcagcg gggccgcgcg tggaaaactt aaatcccatt agggataacc
     8581 cataggtggg aattaaaaga aatttgagca tcttgtcgct ggccgtcccc aaagcggtcc
     8641 gaagcaacag gatccccgat accatgacca agcttttctc gcgtacgtcg atcggccgcc
     8701 ttgccggcaa cagcaagggc gccaccgcca tcgaatatgg cctgatcgcc gccctgatcg
     8761 ccgttgccgc catcaccgcc atgagcggcc ttggcggtca gctgagctcg accttcaaca
     8821 ccacctccag cgccatgggc agcgccgcct cctgataggc ggcgtgctcc acgcgggccc
     8881 gtttccccgg cgggccctgc ggcaggcaaa caggctggcc ccgggcagca catgcgcccg
     8941 gggccttgcc ctgtggcaag ccggctaata gcgcgggaac aaccggcgga tcagctgccc
     9001 gctccacggc aggccgtgat agttgaagaa gaaccagcca aacccccgcg tgaagccgag
     9061 caccaccagc gccagcggat cgcgctgtgc ccgcatgtgg cgaaaaagcg ccagatcggc
     9121 ggcaagcctg tcggcccccg tgcccccgcc attcaggcca taggcattgt catgcgcctt
     9181 gcagccgcga ttgcggtgat agcgacttcc atccataaac aagcaatatt gccgttcctt
     9241 gcccatggcc tggcagtaca cagcgccaat ggccggtttc aagcgcaaac tcttctatct
     9301 gggcggtttc gatccgcggg gcgggcgcta ttatcatgcg cttttcgcgc aggaactggc
     9361 ccgcgcgcaa gggccggcct ggctgggcga tcggcagcgg cgcaagggcg gctgcgggtg
     9421 gctggccggt gatggcgccc agccggcgtg cctgtgcgat tgcacctttc tggcgtggga
     9481 tgatctggtg cggcggcact ggccgcgcgg gctggcgctg gggtgggggc tgtggcgcgc
     9541 ctatggcggc tttgcccgcc acttgcggtg gggcgctctg cacggcgcgc ggccggcggc
     9601 cttgatcgcg ctgttgtggc cgggcctgtg gctgctgctg gcgccggtgg agattggcct
     9661 tgccgcagcg gcgctggtgg cgctgggcgg ggtggcgctg ggggcggccg gcgggctgct
     9721 gctggcgctg ctgtggggcc gggggctgcg gcaggcgcag gcgccctggc tggcgcgctg
     9781 gctgatcttc aacgatcgct gcgctcgccg cc
//
LOCUS       DASRYE010000013        13800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_159398,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000013 DASRYE010000000
VERSION     DASRYE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159398"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFF98_00565"
     CDS             complement(<1..418)
                     /locus_tag="VFF98_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62145.1"
                     /translation="MSGPPSAPVPSTPAPARNTTPDAKPSRQRLVMLALIAAATLINY
                     LDRSVMGVAKPALVADLHINPAVMGLIFSAFSWTYALAQIPGGLVLDRLGTRLTYALS
                     LGLWSAMTALHAAMTGVAGLISARLALGLAEAPCFPA"
     gene            complement(525..1172)
                     /locus_tag="VFF98_00570"
     CDS             complement(525..1172)
                     /locus_tag="VFF98_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU62146.1"
                     /translation="MGEDAQHLSKRQITGIKTRENIIEVAYREFSEKGLAGARIDEIA
                     EQTQTSKRMIYYHFGSKEGLYRAVLERAYETIRTQETAARFADMDAEQALRAIIGHNF
                     DYHFEHPEFVRLVMNENVLKGEHIAQMPGMASRNRPVIDALRAILDKGVAEGVFRTGI
                     DPVDLHMTISALSFYNVSNRYTFLQNFGVDFAAPASRARRRVQIIDCVMHWVKKG"
     gene            complement(1270..2529)
                     /locus_tag="VFF98_00575"
     CDS             complement(1270..2529)
                     /locus_tag="VFF98_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62147.1"
                     /translation="MTAPANPADFAHAGHSGGLFRSLRNHNYRLWVAGAFVSNIGTWV
                     QRTAQDWLVLTMLTHHSASAVGLVMALQFGPQMLLLPWTGVAADRLDQRRLLMITQGA
                     MGVLALALGVLTVTGMVRLWQVDVFAFLLGCAAAFDAPVRQTFVGQLVAQEELSNAVA
                     LNSTSFNGARMIGPAVAGLIIAATGLGWSFVINGVSFFAVLLALMLMRGAELRPIARA
                     SRRAGGFAEGLRYVAARPDLLAILAMLFLIGTFGLNFALFISTMAVQAFHADARGYGL
                     LSSAMAVGTVSGALLNARAGRLRFAMLTNSAALFGIGCLLAALAPGYWWFAAALVLTG
                     MAALSFTNATNSIMQLASEPAMRGRVQALRLGVALGGTPIGAPIVGFVADHAGPRWAL
                     GVGAASGLAAALIGAWVAPRWFARDQA"
     gene            complement(2526..2963)
                     /locus_tag="VFF98_00580"
     CDS             complement(2526..2963)
                     /locus_tag="VFF98_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU62148.1"
                     /translation="MPNATPETARLAQELRRILGGMRRRLREQSAPGDLSPSQSAVLI
                     RLEQDGPATASALARAEGMRAQSMAPVVAGLECAGLLQAEADPADGRQKLYSVTAACH
                     ELLRQRREARQDWLERTIAARLAPEEQARLLEASALLARIIAP"
     gene            3125..4273
                     /locus_tag="VFF98_00585"
     CDS             3125..4273
                     /locus_tag="VFF98_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HZU62149.1"
                     /translation="MADIRKIETILVDLPTIRPHVLAMATMHRQTLCLVRVHCADGIV
                     GLGEATTIGGLAYGPESPETIKTAIDTYFAPLLAGADATRPAAAMALLGRHVVGNHFA
                     KCAVETALLDAQGQRLGVPLSELLGGRRVEHLPVLWTLASGDTARDIAEAEEMIARRR
                     HRTFKLKIGKRALAGDVAHVAAIKAALGDRASVRVDVNMAWDEPTARRGLAMLADAGC
                     DLVEQPIIRHNREGMARLVALGLLPVMADEALTGPASAMDFARTGAADVFAVKIEQSG
                     GLNAARAVAEIAEAAGIGLYGGTMLEGAIGTIASAHAFATFPSLAWGTELFGPLLLTE
                     EILAEPLAYADFGLALPQGPGLGVALDEDRLARFRRDATTTHHTLQGA"
     gene            4277..4564
                     /gene="catC"
                     /locus_tag="VFF98_00590"
     CDS             4277..4564
                     /gene="catC"
                     /locus_tag="VFF98_00590"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HZU62150.1"
                     /translation="MLFQVEMTVHIPLDFDAAEADRIKAEEKAYSQALQASGEWRHIW
                     RVVGQYANVSIFDVASNQRLHEILMGLPLYRFMEMKVTALCQHPSAIHQEG"
     gene            4572..5357
                     /gene="pcaD"
                     /locus_tag="VFF98_00595"
     CDS             4572..5357
                     /gene="pcaD"
                     /locus_tag="VFF98_00595"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZU62151.1"
                     /translation="MEQITTLDGCRIAWRLEGPEDAPVLVLSNSLGTDHAMWDPQMAA
                     LTADYRVLRYDARGHGASDAPAGAYAIDRLGADVLDLMDALGIARAHFCGLSLGGMVG
                     QWLAHRAPQRLGRLVLACTAAQMPPASAWQARIDGVVAQGMAPLAEASIARWFTPGFA
                     ARAPAAIAPIRAMLLATRPQGYAGCCAAIRDMDQRPTARLNACPTLVIAGEADPATSV
                     EQARFLATSAAEGQLAVLPAAHLANVECPDAFSQLVLDHLRGG"
     gene            complement(5354..6256)
                     /locus_tag="VFF98_00600"
     CDS             complement(5354..6256)
                     /locus_tag="VFF98_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62152.1"
                     /translation="MRDIRSLDLNLLKTLDALLEERNVTRAAARLGLTQPAVSAMLAR
                     LREAFEDPLFVRAQRGIVPTLRATAMAGAVKQVLGDIEGLLRPGAFDPATATFTLTLA
                     ATDYALQVIAVPYLSALRRQAPGIRVAVRPMDERPALALFEQGALDLALMTPASAPPD
                     LHARRLFDETYVCAMREGHPAAAAPELSLDAFCALDHALVSYTGDRFRGVTDEALERL
                     GRQRRVVLSVTSFLVLADVLRASDLVAVAPRRLVAGLGGLALREPPLPVAPFTKLAVW
                     HERCHRDAGHQWARAILFETVGEN"
     gene            6366..6866
                     /locus_tag="VFF98_00605"
     CDS             6366..6866
                     /locus_tag="VFF98_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZU62153.1"
                     /translation="MHEIFWPEDYLPGFCDNFASNEVILADLAAADVWPLLAGAPAWP
                     GYYANAANIRFHDGAGPLLALHTRFSFETFGFPVEAEVVEYAAPALGHPGRIAWHGWA
                     GEGASRLDVHHAWLVEELPGGRLRILTQETQKGAPARDLARARPNPMINGHQDWLDGL
                     VAAARR"
     gene            6909..8027
                     /locus_tag="VFF98_00610"
     CDS             6909..8027
                     /locus_tag="VFF98_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HZU62154.1"
                     /translation="MTALFSPFTLKDVTLRNRIAVPPMCQYSAIDGYVTDWHEAHYAG
                     LARGGAGLVIVEATGVSPDGRITPGCTGLWEDGQVAGMARIARAIAAAGAVPGIQIGH
                     AGRKASAYRPWEGDDHIPAGDPAGWETISPSPIAFGGGLPRVPREMSLADIARVQADF
                     VAAAVRAREAGFRWLELHFAHGYLAQSFFSRHANQRTDAYGGDAEGRGRFLLETLAAV
                     RQVWPENLPLTARFGVIEYDGRDEETLAEAIALSARMRAAGLDFLSVSMGFSTIAAKV
                     PWAPAFFAPVVGQVRAALGMPVGSAWCIDAPADAERAVAEGQMDLVMIARAHLADPHY
                     AYAMALALGVEKPAWATLPAPYAHWLSRYRGPGAGAAQ"
     gene            complement(8032..8982)
                     /locus_tag="VFF98_00615"
     CDS             complement(8032..8982)
                     /locus_tag="VFF98_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HZU62155.1"
                     /translation="MPTARRPFIASLLAAPLWAQAVLAEPFAGTYRLAAQPVAPGVWM
                     VRGADQPIAFANGGAIANAAIIATDAGAVLVDCGPSLAYGRALAALARRLTGQAVALV
                     LITHLHPDHALGAGAFDPAIVAALPGTIADIRRDGPGMTDAMFRLLADWMRGTELAVP
                     GRQVAAGPLVVGGRRLQLLALSGHSQADLAVLDEATGTLIAGDLVFHNRAPATPHATL
                     ATWQQALGTLAATPHRQLIPGHGPLDSTGTAIAQTRDWLAWLAAALAEAADHGLEMTE
                     AGDLPIPAGLAAMAEGRYELQRSVSHFYAALESARLPLIG"
     gene            complement(8982..9716)
                     /locus_tag="VFF98_00620"
     CDS             complement(8982..9716)
                     /locus_tag="VFF98_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZU62156.1"
                     /translation="MSRAAPSAAVNADPLGSPMWAMHAARLFGTAPVRFDGAVRVAMP
                     MIAENQHVFPLTVDARALGTVQRIIVLADLNPIPVAVDFAPMALAPWLALRIKLDQRT
                     PVRAAALGADGVWHVAGGWVDAAGGGCSAPPVSRARGDWAEHLGELRGAAFAHADGTR
                     LKFTVRHPMDTGLVENIPAYNIEEITVRAGGRLLAQMAVQGSVAEDPVFSLLLQGPAS
                     APFALRLRDSNGRVFAGDVPPPAGGH"
     gene            9789..10370
                     /locus_tag="VFF98_00625"
     CDS             9789..10370
                     /locus_tag="VFF98_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU62157.1"
                     /translation="MERRAAIGQIAGWALALALAQAGRAGAEEEALFDAEGYRRARYR
                     APVGRAPAPARQIALADALRLSPGRDALFLDVLPAEGGWRDPASGRWQLAEPHETIPH
                     ALWFAETGRAPVAPLLWAALAARVADVRRRHPQWPVVLFCRADCWMSWNAARRLALSG
                     VAGVFWLAEGIEGWHAAGRALRPAQPEAVPGVI"
     gene            10475..11668
                     /locus_tag="VFF98_00630"
     CDS             10475..11668
                     /locus_tag="VFF98_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU62158.1"
                     /translation="MPDASITLDRAFRFDPYSPLIDADPYPAYRRLRDAFPCFWSEEA
                     GMWVLSRHEDVLNALQNWRLYSSAKGNLIDEFPGRAGATLGSSDPPRHDRLRALIQSA
                     MTKRALDHIVAPARAAARARLAAIDGQEEFDFVQDFGAKVTVDLLFHLFNLPPQDAEE
                     VRANAVLMVQTDPVTRQKSEAHLAAFRWMADYAERLVAARKASPGEDLLSAFITAEID
                     GEKLLDKEVQLTVTTLIMAGIESLSGFMGMFALNMAEFAEARRAICADFALLPDAIEE
                     SLRFNTSAQRFKRCATQDIVLHGQTIRAGDFIMLAYGAANRDERVFAEPDRYDIARKP
                     RRHLGFGGGVHACLGAMLARLACQTVFEEFLRAVPEFTRVDGPLAWVPSSNFRSPKGL
                     RLRRG"
     gene            complement(11692..12153)
                     /locus_tag="VFF98_00635"
     CDS             complement(11692..12153)
                     /locus_tag="VFF98_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU62159.1"
                     /translation="MDIDSRDRKILDLLQRDADMPVFRIAELVALSPSACSRRIAALR
                     EAGYIRRTVAVLNRDKLDLPTTVFVILRTQHSEAWLERFRTVVAGIPEILECHRLTGN
                     FDYIIKIVIRNIAEYDRVYKELIAGITLSDVSGYISMETIKEEWSMPVLAR"
     gene            12335..13411
                     /locus_tag="VFF98_00640"
     CDS             12335..13411
                     /locus_tag="VFF98_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZU62160.1"
                     /translation="MRHQSPQGSTWQAWALASLRAEERRSAGTHLRAVPLVGAAGIRL
                     YLKDESGQPTGSLKHRLARALFAYALCNGWIDAGTLVVDASSGSTAVSEAYFAQLLGL
                     PFVAVMPAGTSAEKCALIERYGGRCHFVAEPGAAYAAAAAIAVEQGGCYLNQFAHAER
                     ATDWRRDNIAADILAQLADEPHPEPRWFVCGAGTGGTATTIGRHLRHAGRASQLCVVD
                     PQASVFHRHFYDRAITTAPGPASVIEGIGRQQLEPSFLPDVVDRMIAVSDADAIAAAR
                     HLAAWTGLSCGPSTGANLQGCLVLIDEMRAAGEQGTVVSLLCDAGSRYLSTSLDPAWC
                     AARGLAGGGHPLLARLMPVPAGAR"
     gene            13476..>13800
                     /locus_tag="VFF98_00645"
     CDS             13476..>13800
                     /locus_tag="VFF98_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU62161.1"
                     /translation="MKTQADYVQARIEEKISLGQLRPGQRVEEMSLAQELGTSRTPVR
                     EALAKLVSAGLLQKSPRQGAFVARLDLAEVLGMLEFSAELAAACARFATRRMSPAERQ
                     ELHAHC"
BASE COUNT         2120 a         4674 c         4862 g         2144 t
ORIGIN      
        1 tggcgggaaa acagggcgcc tcggccagcc ccagcgccag ccgcgccgag atgaggccgg
       61 ccaccccggt catcgccgca tgcagcgccg tcatcgccga ccacaggccc agcgacagcg
      121 cataggtcag ccgcgtgccc agcctgtcga ggacgaggcc gccggggatc tgcgccagcg
      181 cataggtcca tgaaaaggcg gagaagatca gccccatcac ggcggggttg atgtgcaggt
      241 cggccaccag cgcgggcttg gccacgccca tcaccgagcg atcgagatag ttgatcagcg
      301 tggccgcggc gatcagcgcc agcatgacga ggcgctggcg gctgggcttg gcgtcaggcg
      361 tggtgttgcg cgcaggtgcc ggcgtgctgg gcacgggcgc ggatggcggg ccgctcatgg
      421 ctcaaatact ctcccggatt atgctgtctt ggcggcaggc ataatcgagg ggcggggctg
      481 tgccaaggaa tatgtacgaa atggtacatt cccggaaggg tgtgtcagcc cttcttcacc
      541 cagtgcatca cgcaatcgat gatctgcacc cggcgcctcg cccgcgaggc gggggcggca
      601 aaatccaccc cgaaattctg caggaaggtg tagcggttgg agacgttgta gaaggagagg
      661 gcgctgatcg tcatgtgcag gtccacaggg tctatgccgg tgcggaacac gccttcggcc
      721 acgcccttgt cgaggatcgc gcgcagggca tcgatcaccg ggcggttgcg cgatgccatg
      781 ccgggcatct gggcaatgtg ttcgcctttc agcacattct cgttcatcac cagccgcacg
      841 aattcgggat gttcgaagtg atagtcgaaa ttgtggccga tgatggcgcg cagggcctgt
      901 tcggcgtcca tatcggcaaa gcgggcggcg gtttcctgcg tgcggatggt ttcataggcg
      961 cgctccagca cggcccggta aagcccttcc ttgctgccga aatggtaata gatcatccgc
     1021 ttggaggtct gcgtctgctc ggcgatctcg tcgatccggg cgccggccag ccctttttcc
     1081 gaaaactccc ggtaggcaac ctcgatgatg ttctcgcgcg tcttgatgcc ggtgatctgc
     1141 cgcttggaga ggtgttgggc gtcttctccc acttatgctg cgctcccgcg aaaggtggcg
     1201 ggctggcccc gccgtctgcc cgattgccta gcgctgtggg cggcggtgtc aaagcaattg
     1261 tcggcccgtt caggcctgat cgcgggcgaa ccagcgcggt gccacccagg cgccgatcag
     1321 tgccgcggca aggccgctgg ccgcccccac gcccagcgcc cagcggggcc cggcatgatc
     1381 ggcaacaaag cccacgatcg gcgcgccgat gggcgtgccg cccagcgcca cgcccagccg
     1441 cagcgcttgc acgcgcccgc gcatggccgg ctcgctggcc agttgcatga tgctgttggt
     1501 ggcattggtg aaggagaggg cggccatgcc ggtgagcacc agcgctgcgg caaaccacca
     1561 gtagcccggc gccagcgcgg ccagcaggca gccaatgccg aacagggcgg cgctgttcgt
     1621 cagcatggca aagcgcaggc ggccggcccg cgcattcagc agcgcgccgc tcaccgtgcc
     1681 caccgccatg gccgaggaca gcaggccata gccgcgcgca tcggcatgga aggcctgcac
     1741 cgccatggtg gagatgaaca gcgcgaaatt gagcccgaac gtgccgatga ggaacagcat
     1801 cgccagaatg gccagcagat cgggccgcgc ggcgacatag cgcagccctt cggcaaagcc
     1861 gcccgcccgg cgcgaggcgc gggcgatggg gcgcagctct gccccgcgca tcagcatcag
     1921 cgccagcagc accgcgaaga acgatacgcc attgatgacg aacgaccagc ccagcccggt
     1981 ggcggcaatg atcagccccg ccaccgccgg gccgatcatc cgtgcgccgt tgaacgaggt
     2041 ggaattgagc gccaccgcat tggaaagctc ctcctgcgct accagctggc cgacgaaggt
     2101 ctgccgcacc ggcgcgtcga aggcggccgc gcagcccagc aggaaggcaa acacatccac
     2161 ctgccacagc cgcaccatgc cggtcacggt cagcaccccc agcgccagcg ccagcacgcc
     2221 catcgcccct tgcgtgatca tcagcaggcg gcgctggtcg agccggtcgg cggccacgcc
     2281 cgtccacggc agcagcagca tctgcgggcc gaactgcagc gccatcacca gccccacggc
     2341 agaggcgctg tggtgggtca gcatggtcag caccagccag tcctgcgcgg tgcgctgcac
     2401 ccaggtgccg atgttcgaga cgaaggcgcc ggccacccac aggcggtaat tgtggttgcg
     2461 cagcgagcgg aacaggccgc cgctgtggcc ggcatgggca aaatcggccg ggttggcagg
     2521 cgcggtcatg gcgcgatgat ccgggcgaga agggcgcttg cctccagcag gcgggcctgt
     2581 tcctcgggcg ccaggcgggc ggcgatggtg cgctccagcc agtcctgccg ggcctcgcgg
     2641 cgctggcgca gcagctcgtg gcaggcagcc gtcaccgaat agagcttctg gcgcccgtcg
     2701 gcggggtcgg cctcggcctg cagcaggccg gcgcattcca gcccggccac caccggcgcc
     2761 atcgactggg cgcgcatgcc ctcggcgcgg gccagggcag aggcggtggc gggcccgtcc
     2821 tgctccagcc ggatcagcac ggccgactgg ctgggcgaca ggtcgccggg cgccgattgt
     2881 tcgcgcaggc gccggcgcat gccgcccagg atgcggcgca gttcctgtgc gagtcgggcg
     2941 gtttcggggg tggcgttggg catggatggc ctatgccata tattcaggca gactgtaaag
     3001 tttacctgaa gaaattccac gcacagccgc aggcgatacc gtggaggtat ggaggcagac
     3061 cgacatggtc tttcctgaac ggcagccgat ggcctatcga agggcgcagg aaaggatttc
     3121 tatcatggcc gacattcgca agatcgagac gattctcgtc gatctgccca ccatccgccc
     3181 gcatgtgctg gcgatggcca ccatgcatcg gcaaacgctg tgcctggtca gggtgcattg
     3241 cgccgatggt atcgtcgggc tgggcgaggc gaccacgatt ggcggcctgg cctatgggcc
     3301 ggaaagcccc gaaacgatca agaccgcgat cgatacctat ttcgccccgc tgctggccgg
     3361 cgccgatgcc acccgcccgg cggcggccat ggccctgctg gggcgccatg tggtgggcaa
     3421 tcattttgcc aaatgcgcgg tggaaacggc gctgctcgat gcgcagggcc agcggctggg
     3481 cgtgccgctg agcgagctgc tgggcggccg gcgggtggag catctgccgg tgctgtggac
     3541 gctggccagc ggcgataccg cgcgcgacat cgccgaggcc gaggaaatga tcgcccgccg
     3601 ccgccaccgc accttcaagc tgaagatcgg caagcgggcg ctggccggcg atgtggccca
     3661 tgtggcggcg atcaaggcgg cgctgggcga tcgggccagc gtgcgcgtcg atgtcaacat
     3721 ggcgtgggac gagccgaccg cgcgccgggg gctggcgatg ctggccgatg ccggctgcga
     3781 tctggtggag cagccgatca tccgccacaa ccgcgaaggg atggcccggc tggtggcgct
     3841 gggcctgctg ccggtcatgg ccgacgaggc actgaccggc cctgccagcg ccatggattt
     3901 tgcccgcacg ggcgcggccg atgtgtttgc cgtgaagatc gagcaatcgg gcggcctcaa
     3961 cgccgcgcgc gccgtggccg agattgctga ggcggccggc attggcctgt atggcggcac
     4021 catgctggaa ggggcgatcg gcaccattgc ctcggcccat gcctttgcca ccttcccgtc
     4081 gctggcctgg ggcacggaac tgttcggccc gctgctgttg accgaggaga ttctggcaga
     4141 gccgctggcc tatgccgatt tcggcctggc gctgccgcag ggcccgggcc ttggcgtggc
     4201 gctggacgaa gaccgcctgg cccgcttccg ccgcgatgcc accaccaccc accacaccct
     4261 ccagggagcc tgagccatgc tgttccaggt ggaaatgacc gtgcacatcc ccctcgattt
     4321 cgatgccgcc gaggccgatc gcatcaaggc cgaggaaaag gcctattccc aggccctgca
     4381 ggccagcggt gaatggcgcc atatctggcg ggtggtgggc cagtatgcca atgtcagcat
     4441 cttcgatgtg gccagcaacc agcgcctgca cgagatcctg atgggcctgc cgctctatcg
     4501 cttcatggag atgaaggtga cggcgctgtg ccagcacccc tcggccatcc accaggaggg
     4561 ttgagcgggc gatggaacag atcaccacgc tggatggctg ccggatcgcc tggcggctgg
     4621 aggggcccga ggacgcgccg gtgctcgtgc tgtcgaactc gctgggcacc gatcatgcca
     4681 tgtgggatcc gcagatggct gcgctgaccg ccgattatcg cgtgctgcgc tatgatgccc
     4741 gcggccatgg cgcatcggat gcgccggcgg gcgcctatgc gatcgaccgg ctgggcgccg
     4801 atgtgctgga tctgatggat gcgctgggca ttgcgcgggc gcatttctgc gggttgtcgc
     4861 tgggcggcat ggtggggcaa tggctggcgc atcgggcgcc gcagcggttg ggccggctgg
     4921 tgctggcctg cacggccgcg cagatgccgc ccgcctctgc ctggcaggcg cgcatcgatg
     4981 gcgtggtggc gcagggcatg gcgccgctgg ccgaggcctc tatcgcccgc tggttcacgc
     5041 ccggctttgc cgcccgcgcg ccggcggcca tcgcccccat ccgcgccatg ctgctggcca
     5101 cccgcccgca aggctatgcc ggctgctgcg cggccatccg cgatatggac cagcgcccca
     5161 ccgcccgcct caacgcctgc cccacgctgg tgatcgccgg cgaggcggac ccggccacct
     5221 cggtggagca ggcccgcttc ctggccacca gcgcggcgga agggcagctg gcggtgctgc
     5281 cggcggcgca tctggccaat gtggaatgcc cggacgcgtt ctcgcagctg gtgctggatc
     5341 atctgcgcgg cggctagttc tcgcccactg tctcgaacag gatggcgcgg gcccattggt
     5401 ggccggcatc gcggtggcag cgctcgtgcc acacggccag cttggtgaag ggcgccaccg
     5461 gcagcggcgg ctcgcgcagg gccagcccgc caaggcccgc caccagccgg cgcggcgcca
     5521 ccgccaccag atcgctggcg cgcagcacat cggccagcac cagaaagctg gtgaccgaaa
     5581 gcaccacccg ccgctggcgc cccagccgct ccagcgcctc atccgtcacc ccgcgaaagc
     5641 gatcgcccgt ataggaaacc agcgcgtgat cgagcgcgca gaaggcatcg agcgacagct
     5701 ctggcgccgc tgccgccgga tggccctcgc gcatcgcgca gacataggtc tcgtcgaaca
     5761 ggcggcgggc gtggagatcg ggcggggcgc tggccggcgt catcagcgcc agatccagcg
     5821 cgccctgctc gaacagcgcc agcgccgggc gctcgtccat cggccgcacc gccacgcgga
     5881 tgcccggcgc ctgccgcctc agcgccgaca gatagggcac cgcgatcacc tgcagggcat
     5941 aatcggtggc ggcgagggtg agggtgaagg tggccgtggc gggatcaaaa gcgcccggcc
     6001 gcagtagtcc ctctatgtcg cccagcacct gcttcaccgc gcctgccatc gccgttgccc
     6061 gcaaggtggg cacgatgccg cgctgcgcgc gcacgaacag cggatcctcg aaagcctcgc
     6121 gcaggcgcgc cagcatggcg ctgaccgccg gctgggtgag gcccagccgg gcggcggcgc
     6181 gggtcacgtt gcgttcttcc agcagcgcgt cgagcgtttt gagcaggttg agatcgagcg
     6241 atctgatatc acgcatggca atgcccatga tccatatttg cgatttctct tatatcttgc
     6301 gcaggcctat gcgccgccca tccgcccgtc aatggcccgg ggtccggcaa accaaggaca
     6361 tatccatgca cgagatcttc tggccggagg actatctccc cggcttttgc gacaattttg
     6421 cttccaatga agtgatcctg gccgatctgg ccgcggccga tgtgtggccg ctgctggccg
     6481 gcgcaccggc ctggccgggc tattatgcca atgcggccaa cattcgcttc cacgatgggg
     6541 cagggccgct gctggcgctg cacacgcgct tttccttcga gactttcggc tttccggtgg
     6601 aggccgaggt ggtggaatat gcagcgcccg cgctgggcca tccgggccgc attgcctggc
     6661 atggctgggc gggcgagggg gcaagccggc tcgatgtgca ccacgcctgg ctggtggagg
     6721 aattgccggg cgggcgcctg cgcatcctca cccaggaaac ccagaaaggc gcgcccgccc
     6781 gcgatctggc cagagcccgc cccaatccga tgatcaacgg ccatcaggat tggctggacg
     6841 ggctggtggc cgccgcccgc cgctgatccg cttcccccgc cttccccccc aacgcgagtg
     6901 cttgcccgat gacagccctg ttcagcccct tcacgctcaa agacgttacg ctgcgcaacc
     6961 gcatcgccgt gccgccgatg tgccaataca gcgccatcga cggctatgtg accgactggc
     7021 acgaggccca ttacgccggc cttgcccgtg gcggcgccgg gctggtgatc gtcgaggcga
     7081 ccggcgtgtc gcccgatggc cgcatcacgc ccggctgcac cggcctgtgg gaggatgggc
     7141 aggtggccgg catggcccgc atcgcccgcg cgattgccgc ggcaggcgcg gtgcccggca
     7201 tccagatcgg ccatgccggc cgcaaggcca gcgcctaccg cccgtgggaa ggcgacgacc
     7261 acatccccgc tggcgatccg gccggctggg agacgatctc gccttcgccc atcgcctttg
     7321 gcggcggcct gccccgcgtg ccgcgcgaga tgagcctggc cgatatcgcc cgggtgcagg
     7381 ccgatttcgt ggcagcggcg gtgcgcgcgc gtgaggccgg ctttcgctgg ctggagctgc
     7441 atttcgccca tggctatctg gcgcaaagct tcttttcccg ccacgccaac cagcgcacgg
     7501 atgcctatgg cggcgatgcc gaagggcgcg ggcgcttcct cctcgaaacg ctggcggcgg
     7561 tgcggcaggt gtggccggaa aacctgccgc tgacagcccg cttcggggtt atcgaatatg
     7621 acgggcgcga cgaggaaacg ctggccgagg cgattgccct gtctgcccgc atgcgcgctg
     7681 cggggctgga tttcctcagc gtttccatgg gcttttccac gatcgcggcc aaggtgccat
     7741 gggcgccggc cttcttcgcg ccggtggtgg gccaggtgcg cgcggcgctg ggcatgccgg
     7801 tgggctctgc ctggtgcatc gatgcgccgg ccgatgccga aagggcggtg gccgaagggc
     7861 agatggatct ggtgatgatc gcccgcgccc atctggccga tccgcattat gcctatgcga
     7921 tggccttggc gctgggggtg gaaaagcccg cctgggccac cctgccggcg ccctatgccc
     7981 attggctgtc gcgctatcgc gggccggggg cgggggcggc ccagtaagcg gtcagccgat
     8041 aaggggcagg cgggcgcttt ccagcgcggc gtagaaatgc gagacgctgc gctgcagctc
     8101 gtagcgcccc tcggccatgg cggcgaggcc tgcggggatc ggcagatcgc ccgcctcggt
     8161 catttccagc ccgtggtcgg ccgcctcggc cagggcggcg gccagccagg ccagccagtc
     8221 gcgcgtctgg gcgatggcgg tgcccgtgct gtcgagcggg ccgtggccgg ggatcaactg
     8281 gcggtggggc gttgccgcca gcgtgcccag cgcctgctgc caggtggcga gcgtggcatg
     8341 gggcgtggcc ggcgcccggt tgtgaaacac cagatcgccg gcgatcagcg tgccggtggc
     8401 ctcgtccagc acggcgagat cagcctggct gtggccagag agcgccagca gctgcaggcg
     8461 ccgcccgccc accaccagcg gccctgccgc cacctgccgg cccggcaccg ccagctctgt
     8521 gccgcgcatc cagtcggcca gcaggcggaa catggcgtct gtcatgcccg gcccgtcgcg
     8581 ccggatatcg gcaatggtgc cgggcagggc ggcaacgatg gcgggatcga aggcgcccgc
     8641 ccccagcgca tgatcggggt gcagatgggt gatgagcacc agcgccaccg cctggccggt
     8701 gagcctgcgc gccagcgccg ccagcgcccg cccataggcc agcgagggcc cgcaatcgac
     8761 cagcaccgcg ccggcatcgg tggcgatgat cgcggcattg gcgatcgccc cgccattggc
     8821 aaaggcgatc ggctgatcgg ccccgcgcac catccacacc cccggcgcca caggctgggc
     8881 ggccagccga taggtgccgg caaagggctc tgccagcacg gcctgtgccc acaggggcgc
     8941 ggccagcagg ctggcgatga acgggcggcg ggcggtgggc atcagtggcc tcctgcaggc
     9001 ggcggcacat cgccggcaaa cacgcgcccg ttgctgtcgc gcaggcgcag cgcgaagggg
     9061 gcgctggccg ggccctgcag cagcagcgag aacaccggat cttctgccac cgagccctgc
     9121 accgccattt gcgccagcag gcggccgccc gcgcgcacgg ttatttcctc gatgttgtag
     9181 gcggggatat tctcgacaag gccggtgtcc atcgggtggc gcacggtgaa tttcagcctt
     9241 gtgccatcgg catgggcaaa ggcagcgccg cgcaattcgc ccagatgttc cgcccaatcc
     9301 ccccgcgccc ggctgaccgg cggcgccgag cagccaccgc cggccgcatc cacccagccg
     9361 cccgccacat gccacacgcc atctgcgccg agggcggcgg cgcgcaccgg cgtgcgctgg
     9421 tccagcttga tgcgcagcgc cagccagggc gccagggcca tcggcgcgaa atccaccgcc
     9481 accgggatcg ggttgagatc ggccagcacg ataatccgct gcaccgtgcc cagcgcccgc
     9541 gcatccaccg tcagcgggaa gacatgctgg ttttccgcga tcatcggcat ggccacgcgc
     9601 actgcgccat caaagcgcac gggcgctgtg ccaaacagcc tggccgcatg catcgcccac
     9661 atcggcgagc ccagcggatc tgcgttgaca gcagcggaag gggcggcgcg gctcatacca
     9721 aagtggcata ctgcgccgcg ccccgccttt tatattcgac ctaggaacaa ggcagaacgg
     9781 cggcagggat ggagaggcgg gcggcaatcg ggcagatcgc gggctgggcg ctggcattgg
     9841 cactggcgca ggccggccgc gccggcgctg aggaggaagc gctgttcgat gccgagggct
     9901 atcgccgcgc tcgctatcgc gcgccggtgg gccgcgcgcc tgcccctgcc cgccagattg
     9961 cgctggccga tgcgctgcgc ctgagccctg ggcgcgatgc gctgtttctc gatgtgctgc
    10021 cggccgaagg gggctggcgc gatcccgcca gtggccgctg gcaactggcc gagccgcatg
    10081 agacgatccc ccacgccctg tggtttgccg aaaccggccg cgcgccggtg gcgcccctgc
    10141 tgtgggcggc gctggccgcg cgggtggccg atgtgcgccg gcgccatccg cagtggccgg
    10201 tggtgctgtt ttgccgcgcc gattgctgga tgagctggaa tgccgcgcgc cggctggcgc
    10261 tttccggcgt ggccggggtg ttctggctgg ccgaggggat cgagggctgg catgcggccg
    10321 gccgcgcgct gcggcctgcc cagccggaag cggtgcccgg ggtgatttga gtgaaaagta
    10381 caagccattt cccgaaacgc ctctcacgca tttgccacaa tccgcgttaa tccctctcca
    10441 agcctgcatg ggcaggcaat ggagagggcg aataatgcca gatgcatcca tcacgctcga
    10501 cagggcgttc cgctttgatc cctattcccc gctgatcgat gccgatccct atccggccta
    10561 tcggcgcctg cgcgacgcat ttccctgctt ctggagcgag gaggcgggca tgtgggtgct
    10621 cagccggcac gaggatgtgc tgaacgcgct gcagaactgg cggctctatt cctcggccaa
    10681 gggcaatctc atcgacgaat ttcccggccg cgccggggcc acgctgggca gcagcgatcc
    10741 gccacgccac gaccggctgc gcgcgctgat ccagtcggcg atgacgaagc gcgcgcttga
    10801 tcacatcgtg gcgcccgcgc gcgctgccgc ccgcgcccgg ctggccgcga tcgacgggca
    10861 ggaggagttc gatttcgtgc aggatttcgg cgcgaaggtg acggtggacc tgttgttcca
    10921 cctgttcaac ctgccgccgc aggatgccga ggaagtgcgc gccaatgccg tgctgatggt
    10981 gcagacagac cctgtgaccc gccagaagag cgaggcccac ctcgccgcct tccgctggat
    11041 ggccgattat gccgaaaggc tggtggccgc gcgcaaggcc agccccggcg aggatctgct
    11101 ctccgccttc atcaccgccg agatcgatgg cgagaagcta ctcgacaagg aagtgcagct
    11161 tacggttacc acgctgatca tggcggggat tgaatcgctg tcgggcttca tgggcatgtt
    11221 cgccctcaat atggcggaat ttgccgaggc gcgccgggcg atctgcgccg atttcgccct
    11281 gctgcccgat gccatcgagg aaagcctgcg cttcaacacc tcggcccagc gtttcaagcg
    11341 ctgcgccacg caggatatcg tgctgcacgg gcagaccatc cgcgccggcg atttcatcat
    11401 gctggcctat ggcgcggcca accgcgacga gcgcgtcttc gccgagcccg atcgctatga
    11461 catcgcccgc aagccccggc gccacctggg ctttggcggg ggtgtgcatg cgtgcctggg
    11521 cgcgatgctg gcgcggctgg cctgccagac ggtgttcgag gaattcctgc gcgcggtgcc
    11581 ggaattcacc cgggtggatg ggccgctggc ctgggtgccc tcgtcgaact tccgcagccc
    11641 caagggcctg cgcctgcgcc gcggctgagg ggccgggccg gtgctggcct gtcagcgggc
    11701 cagcaccggc atgctccatt cctccttgat ggtttccatc gagatatagc ccgacacatc
    11761 cgacagggtg atgccggcga tcagttcctt gtagacccgg tcgtattcgg cgatgttgcg
    11821 gatgacgatc ttgatgatgt agtcgaaatt gccggtcagc cggtggcatt cgaggatttc
    11881 ggggatgccg gccaccaccg tgcggaagcg ctccagccag gcctcgctgt gctgcgtgcg
    11941 caggatgacg aagacggtgg tcggcagatc cagcttgtcg cggttgagca cggccaccgt
    12001 gcggcggatg tagccggcct cgcgcagggc ggcaatgcgg cgcgagcagg ccgagggcga
    12061 cagcgccacc agctcggcga tgcggaacac cggcatatcg gcatcgcgct gcagcagatc
    12121 gaggatcttg cggtcgcggc tatcgatatc catcgcgaat ctcctgcatg agggggcctt
    12181 ctaggcatcg cacgggcatg cgcaaaagca agcgtgtatg cacgggccat gcaaaagcat
    12241 gcaaaatggc gcccgccttt gcaccccaaa cgcgggcggg caacgctaga ttccgtggcg
    12301 aaggcggcac tggcgccgca gggcaggaac acggatgagg caccaatccc cgcaaggcag
    12361 cacgtggcag gcatgggcgc tggccagcct gcgtgctgaa gagcgccgct cggccggcac
    12421 ccatttgcgc gcagtgccgc tggtgggcgc ggccggaatc cggctctatc tcaaggatga
    12481 atccggccag cccaccggca gcctgaagca ccggctggcc cgcgccctgt tcgcctatgc
    12541 cctgtgcaat ggctggatcg atgccggcac gctggtcgtc gatgcctcct cgggctccac
    12601 cgccgtgtcg gaggcctatt tcgcgcagct gctgggcctg cccttcgtgg cggtgatgcc
    12661 ggccggcact tctgcggaaa aatgcgcgtt gatcgagcgc tatggcgggc gctgccattt
    12721 cgtggcggag ccgggggcgg cctatgccgc tgccgctgcc atcgctgtgg agcagggcgg
    12781 ctgctatctc aaccagttcg cccatgccga gcgcgccacc gactggcggc gcgacaatat
    12841 cgccgccgac atccttgccc agctggccga cgagccccat cccgagccgc gctggttcgt
    12901 gtgcggcgcc ggcaccgggg gcacggccac caccattggc cgccacttgc gccatgccgg
    12961 ccgcgccagc cagctgtgcg tggtggatcc gcaggcctcg gtgttccacc gccatttcta
    13021 cgatcgcgcg atcaccaccg cgcccgggcc cgcctcggtg atcgagggga ttggccggca
    13081 gcagctggag ccttccttcc tgcccgatgt ggtggaccgg atgatcgccg tcagcgatgc
    13141 cgacgcgatt gccgccgcgc gccatctggc ggcctggacg ggcctgtctt gcgggccgtc
    13201 cacgggcgcc aacctgcagg gctgcctggt gctgatcgac gagatgcgcg cggctggcga
    13261 gcagggcacg gtggtcagcc tgctgtgcga tgccggcagc cgctatctgt cgacgagcct
    13321 cgatccggcc tggtgcgcgg cgcgcgggct ggcgggcggc ggccatccgc tgctggcccg
    13381 gctgatgccc gttccggctg gcgcgcgttg acgtggcata catggatata taccgtgcat
    13441 gcaatggcgc tatcagcgcc gcggcagggg gcatgatgaa aacacaggcg gattatgtgc
    13501 aggcccggat cgaggagaag atctcgctgg ggcaattgcg gccgggccag agggtggaag
    13561 agatgagcct ggcgcaggag ctgggcactt cccgcacgcc cgtgcgcgag gcgctggcca
    13621 agctggtgtc ggccggcctg ttgcagaaat cgccgcggca gggggctttc gtggcccggc
    13681 tcgatctggc cgaagtgctt ggcatgctgg agttttctgc cgaactggcg gcggcctgcg
    13741 cgcgctttgc cacgcgccgc atgtcgccgg ccgagcggca ggagctgcac gcccattgcg
//
LOCUS       DASRYE010000014         9763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_212784,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000014 DASRYE010000000
VERSION     DASRYE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212784"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..950
                     /locus_tag="VFF98_00650"
     CDS             180..950
                     /locus_tag="VFF98_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62162.1"
                     /translation="MPQPAYFTAPTGLTIAHIHAAPTDPAAPTLVFLPGYMSDMSGSK
                     ATALFDWAWARGHGCLLLDYSGCGQSEGQFAQGTLSRWRAEVVALIQAQVAGPVVLVG
                     SSMGGWLMLLVAEALPAGKVAALVGIAAAPDFTDWGYAPEQKALLAAGDTVLEPNPYG
                     PEPTPTHAAFWADGQANRLLGRTIRFDGPVRLLQGMADADVPWHTAPALALALRSAQV
                     QVHLVKDGDHRLSRPADIALLLGAVADALAAACSDPPC"
     gene            969..1898
                     /locus_tag="VFF98_00655"
     CDS             969..1898
                     /locus_tag="VFF98_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62163.1"
                     /translation="MPSLAASLFAALLPFVAQASLGSGAGASLDIPKVDRAPNAVNPR
                     HKGEAYLAADLAPRPKSRLDTCLDDTNTDPEAAEQTARAWLQEEKGSKQAEPQLCLGS
                     AEAAMGNWAEARAAFTAGRAIAATSDKVLKARLAAMAGNAALADGDGAGALALIDTAH
                     AEAKALHAPRLAGGMAIDRARALVALGRNEEAVLSLAEAREALPNDAAAWLLSATLAR
                     RMNKLGMAESEIVTAAQLAPTDPAIGLEAGVIAMLAGHEAAARKSWQSAITTAPDSAE
                     AKQARAYLDQLGSAPAAPASAPAPSAAPGRHER"
     gene            1996..3234
                     /locus_tag="VFF98_00660"
     CDS             1996..3234
                     /locus_tag="VFF98_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU62164.1"
                     /translation="MSETKNFQADVIIVGGGHAGAQCAIALRQNGFTGSVAMIGRESE
                     PPYERPPLSKEYFAREKTFDRLYIRPPQFWAEKHVDLHLGREVIAIDPVAKTLDLADG
                     ATATYGKLVWAAGGDPRKLSCAGADLAGVHAVRTREDCDRLMGEIDGGVKHIAVIGGG
                     YIGLEAAAVLTKMGCSVTLLEALPRVLARVAGPELSAFYEAEHRAHGVDLRTGVAVDS
                     LVGEGRVSGVKLADGTIIPADAVIVGIGIVPAVGPLFAAGAAGGNGVDIDAYCRTSLP
                     DVYAVGDCAAFACDFAEGTVMRVESVQNANDQATCVAKAICGEEKPYHAFPWFWSNQY
                     DLKLQTAGLSVGYDTTVLRGDPAARAFSVVYLKAGRVIAIDSVNVVKDYVQGRKLVEA
                     GATPDVARLADTSVPLKELL"
     gene            complement(3254..4339)
                     /gene="queG"
                     /locus_tag="VFF98_00665"
     CDS             complement(3254..4339)
                     /gene="queG"
                     /locus_tag="VFF98_00665"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZU62165.1"
                     /translation="MVNAALNDFKQALFAEARALGFAAVGVAPAAEDAARAARLEAWL
                     EDGRHGQMGWMADRLHHRRSPQGLWPEAASVVALGLSYAPATDPLALADVADRARIST
                     YAQGADYHDVVKKALKALARWMVEQAPRRGLGEIGVKVFVDTAPVMEKPLGAAAGLGW
                     QGKHTNMVSRAHGSWLFLGAIYTTLALPAEAPGADRCGSCSACQQACPTGAFPAPYQL
                     DARRCVSYLTIEHKGPVAEDLRAGLGNRIYGCDDCLAVCPWNKFAATAARHKAFLPRA
                     ELVAPRLADLLALDDAGFRRLFSGSPIKRIGRDRFVRNCLYAAGNSGDGALAAPVAAL
                     LEDGDEAVADAARWALAKLAGRGQGQG"
     gene            4431..5381
                     /locus_tag="VFF98_00670"
     CDS             4431..5381
                     /locus_tag="VFF98_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62166.1"
                     /translation="MSAGASALAIEAHGLVKRFDGTVAVAGVDLAVPQGAIYGVLGPN
                     GAGKTTTLRMLLGIIEPDAGTRRVLGAANPQAMAHAIGYLPEERGLYPTMKAFEAIAF
                     LGALRGQPLAEGRRRALELLEAQGLSHAADRQIRHLSKGMAQQVQLLGTLVHRPQLVV
                     LDEPFSGLDAINQGRLEAMIRDLATGGTTVLFSTHVIAHAERMCDEVAIIAGGRVPFA
                     GAVDVARDRIAPQVMLETRHPDGPWRAALPADARAEGRRWHFALPPSGVEPLLRALIE
                     GEAGIVSLSIERAGLHDAFIAIAGAAAADAMATPAAEEAA"
     gene            5378..6577
                     /locus_tag="VFF98_00675"
     CDS             5378..6577
                     /locus_tag="VFF98_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU62167.1"
                     /translation="MNAPHPHHGRLSLLAAARVIARRDFIAILWSRSFLFFLLGPLFP
                     AVVGLLAGTVGSQANDSIGPPVIGVMMSRADTAAMLAAEADLAPHLADALPQLVPLGA
                     GAPASHAQVAAMISGTPAHPVLAGPGPAIEAWQGPVALLAARADGHAAIRFAPVRLAP
                     TATSTADTSHARLLTAQAAQTLLLLLTMLLAGMVLSNLVEEKANKIIEILAAAIPMDA
                     VFLGKLVAMLGVSLVGIAVWGAAGALLHLAAGQALPALTAPAVGWPLFVALGIAYFAM
                     GYLLLGSVFLAIGSLASTVREVQTLSMPATMMQILVFFFAGYAIARPGSGAELAAMVF
                     PFSSPFTMVARAALQPALWPHVAALGWQALWVALLVRWGAALFRRRVMKSGPQAAPRR
                     RRWFARG"
     gene            6646..8385
                     /locus_tag="VFF98_00680"
     CDS             6646..8385
                     /locus_tag="VFF98_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62168.1"
                     /translation="MPTPVPSGPRHAAWAPVLWLILLLAAGLGAALVDYPIATLYFRL
                     QDRWLAPLSALLLLAGLVAGWAVMRGRVAPRRPGARSMAVLALAVLALGWAGHRWLLA
                     DYDLSRDEQMAVFDAFIYAHGHLVWPLPAAWRGDAPALNLLFMLPVARPVAWVSTYLP
                     IHALLRAGMGLAGAAALTSPLLNAIAVLATWGAARRLFGAAEREGAGLAAVLLATSGQ
                     FLFAGMSAYAMAAHLACNMVWLWLFLADRRRADGAALAVGFLATGLHQPLFHPLFVAP
                     FLLLLAARRRWGRLALFVLAYLPIGLFWLAWPHFTHDLVAGTGSVSQSGTSFAERLRD
                     VLAANTQNLPLMAENLLRFAAWQAPVWGPLALIGGWAAWRQRQGEMLALAAGLVAPAL
                     LMAALLPFQGHGFGYRYLHGVLGNGALLAAWGWVALGDRRQRWRAGLVAALAAGALVL
                     LPVEAGMAHALYAPFAAASARLDASGADYVLIGADEAPLALDLVRNRPDLANRPLRLS
                     TIDIDPMEPLAARLCGAAPAGHPMRVGLPVDSFYAGINAALGMPPAHVADRRFAEDAD
                     AFSDAGCTVAALR"
     gene            complement(8377..8805)
                     /locus_tag="VFF98_00685"
     CDS             complement(8377..8805)
                     /locus_tag="VFF98_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZU62169.1"
                     /translation="MSRETALAPRIRALIDREPLRYLLCGGLCAVVNNIILIGGDGLG
                     LHYALLTAISWALAGSLGYGLNARFTFRQPWRGRAYLRFMGGVALGVPLSLALLALFV
                     SLLRLPMWFAAPASTLLMMVYNYANARLAIMRRLLGKGSA"
     gene            complement(8802..>9763)
                     /locus_tag="VFF98_00690"
     CDS             complement(8802..>9763)
                     /locus_tag="VFF98_00690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62170.1"
                     /translation="GMSLPLQGQPWLSVVMPIHGGATYLGATLASVAAEAPQGVEFLC
                     YDSGADGGQARAIAEAHADQLAIRWADVPDIRPWTAKVNLGVGHASAPHIAILHQDDL
                     WLPGHLAALRASLALAGDAVLSVGPSRLVGPAGQDVGLWRLPLRQGRQQGGDVATRLL
                     TQNTIAVPAPLFRRDAFLAAGGMDDALWYTADWDLWLKLARRGDVFVRAGATTAFRLH
                     GGSLTMTGSRDLADFRQQMERVLDAHAPQLAPPAAALARARASVALNVALAGVSAGDR
                     SALMGALGQVARLGPLGAWRFCQETQIIGRLWPRVKLMLAGKL"
BASE COUNT         1353 a         3465 c         3452 g         1493 t
ORIGIN      
        1 gcggccatcg cgcgcgtgga accatcggga agggtgatcg tgaacatctc ggacatggcg
       61 cccgccttag ccgccggcgc ggcgcggctc aaccttgtat cgcgcggggt atcgcgcagg
      121 ggcccctgcc accttgcggt cttgtcactg gcggcaatcc gtcgcatcac tggtccgcca
      181 tgccccagcc cgcctatttc accgccccca ccgggctcac catcgcccat atccatgccg
      241 cccccaccga tccggccgcg cccacgctgg tgttcctgcc cggctatatg agcgacatga
      301 gcggatcaaa ggccaccgcc ctgttcgact gggcctgggc caggggccat ggctgcctgc
      361 tgctggatta ttccggctgc gggcaaagcg agggccagtt tgcgcaaggc acgctcagcc
      421 gctggcgcgc agaagtggtg gcgctgatcc aggcgcaagt ggcagggccg gtggtgctgg
      481 tgggctcgtc gatggggggc tggctgatgc tgctggtggc agaggcgctg ccggccggca
      541 aggtggccgc gctggtgggc attgccgccg cgcccgattt taccgactgg ggctatgcgc
      601 ccgaacagaa ggccctgctg gccgccggcg acaccgtgct ggagcccaac ccctatgggc
      661 cagagcctac ccccacccat gccgccttct gggccgatgg ccaggccaac cggctgctgg
      721 gccgcaccat ccgctttgat gggccggtgc gcctgctgca gggcatggcc gatgccgatg
      781 tgccctggca caccgccccg gcgctggccc tggcgctgcg ttcggcgcag gttcaggtgc
      841 atctcgtcaa ggacggcgac catcgcctca gccgcccggc cgatatcgcc ctgctgcttg
      901 gggcggtggc cgatgcgctg gcggcggcct gttccgatcc gccctgctga aagagtgttc
      961 gcgcccccat gccctccctt gccgcttccc tgtttgccgc cctcctgccg tttgtggccc
     1021 aggccagcct gggcagcggc gccggcgcca gcctcgatat acccaaggtt gatcgcgcgc
     1081 ccaatgcggt gaacccgcgc cacaagggcg aggcctatct ggccgcggat ctggcaccgc
     1141 ggcccaaaag ccggctcgac acctgccttg atgacaccaa caccgatccc gaagcggccg
     1201 agcagaccgc ccgcgcctgg ctgcaggaag agaagggctc caaacaggca gagccgcagc
     1261 tgtgcctggg cagcgccgag gcggcgatgg gcaattgggc cgaagcgcgc gccgccttca
     1321 ccgccgggcg cgccattgcc gctacttcgg acaaggtgct gaaggcgcgg ctggccgcga
     1381 tggccggcaa tgccgccctt gccgatggcg atggcgccgg cgcgctggcg ctgatcgaca
     1441 ccgcccatgc cgaggccaag gcgctgcacg cgccgcgtct ggccggcggc atggcgatcg
     1501 accgggcccg cgcgctggtg gcgctgggcc gcaacgagga ggcggtgctc tcgctggccg
     1561 aagcgcgcga ggccctgccc aacgatgccg ccgcctggct gctgtcagca acgctggcgc
     1621 ggcggatgaa caagctgggc atggccgaaa gcgagatcgt caccgccgcc cagctggccc
     1681 ccaccgatcc cgccatcggc ctggaggcag gcgtgatcgc catgctggcc gggcatgagg
     1741 cggccgcccg caaaagctgg caatcggcca tcaccaccgc gcccgacagc gccgaggcca
     1801 agcaggcccg cgcctatctc gaccagctgg gcagcgcgcc tgccgcccct gcgtctgccc
     1861 ccgccccctc tgccgccccc ggccggcacg aacgctagaa ggcttgcccc ctgcggccaa
     1921 agcatcacgc ctgtgccggc aagatgcacg cagcgcattg acggcgccgc tcattttgcc
     1981 cacacagctg cgcccatgag cgagacaaaa aatttccagg cggatgtcat catcgtgggc
     2041 ggcgggcatg cgggggcgca atgcgccatt gccctcaggc aaaacggctt taccggcagc
     2101 gtggccatga tcggccgcga gagcgagccg ccttacgagc gcccgccgct ctccaaggaa
     2161 tatttcgccc gcgagaagac cttcgaccgc ctctatatcc gcccgccgca gttctgggcg
     2221 gaaaagcacg tggatctgca tctcggccgc gaagtgatcg ccatcgatcc ggtggccaag
     2281 acgctggatc tcgccgatgg cgccacggcc acctatggca agctggtgtg ggcggccggc
     2341 ggtgatccgc gcaagctctc ctgcgcgggg gcggatctgg ccggcgtgca tgccgtgcgc
     2401 acccgcgagg attgcgaccg gctgatgggc gagatcgatg gcggcgtgaa gcatatcgcg
     2461 gtgatcggcg gcggctatat cgggctggaa gcggcggctg tgctcaccaa gatgggctgt
     2521 tccgtcaccc tgctggaagc gctgccgcgg gtgctggcgc gcgtggcggg gcctgagctt
     2581 tcggcgttct acgaggccga gcaccgcgcc catggggtgg atctgcgcac cggcgttgcg
     2641 gttgattcgc tggtcggaga aggccgggtc agcggcgtga agctggccga tggcacgatc
     2701 atcccggccg atgcggtgat cgtgggcatt ggcatcgtgc cggcggtggg cccgctgttt
     2761 gccgcgggcg ctgccggcgg caatggcgtt gatatcgatg cctattgccg cacctcgctg
     2821 cccgatgtct atgccgtggg cgattgcgcg gcctttgcct gcgattttgc cgagggcacg
     2881 gtgatgcggg tcgaatcggt gcagaacgcc aatgatcagg ccacctgcgt ggccaaggcc
     2941 atctgcggcg aggaaaagcc ctatcacgcc ttcccgtggt tctggtcgaa ccagtatgac
     3001 ctgaagctgc agaccgccgg gctttcggtg ggctatgaca ccaccgtgct gcgcggcgat
     3061 ccggcggcgc gggcgttcag cgtggtctat ctgaaggccg gccgcgtgat cgccatcgac
     3121 agcgtgaatg tggtgaagga ctatgtgcag ggccgcaagc tggtggaagc gggcgccacg
     3181 cccgatgtcg cccggctggc cgacacttcg gtgccgctga aggaactgct gtaaggccag
     3241 gggggcgggg gttttacccc tgcccctggc cccttccggc cagtttcgcc aaggcccagc
     3301 gcgccgcatc ggccacggcc tcgtcgccat cttccagcag cgctgccacc ggcgctgcca
     3361 gcgccccatc gccgctgttg ccggcggcat agaggcagtt gcgcacgaac ctgtcccgcc
     3421 cgatccgctt gatcggcgag ccggagaaca ggcggcgaaa gcccgcatcg tccagcgcca
     3481 acagatcagc caggcgcggc gccacgagtt cggcgcgggg caggaaggcc ttgtggcgcg
     3541 cggccgtggc ggcaaacttg ttccacggac acacggccag gcaatcgtcg cagccataga
     3601 tgcggttgcc cagccctgcg cgcagatcct cggccaccgg ccccttgtgc tcgatggtga
     3661 gataggagac gcagcgccgc gcatccagct gatagggcgc ggggaaagcg ccggtggggc
     3721 aggcctgctg gcaggcgctg cacgagccgc agcgatcagc ccccggcgcc tctgccggca
     3781 gcgccagcgt ggtatagatc gcccccagga acagccagct gccatgcgcg cggctgacca
     3841 tattggtgtg cttgccctgc cagcccaggc ccgcggccgc gcccagcggc ttttccatca
     3901 ccggcgctgt atcgacaaac accttcacgc caatctcgcc cagcccgcgc ctgggggcct
     3961 gctccaccat ccagcgggcc agcgccttca gcgccttttt caccacatcg tggtaatcgg
     4021 ccccttgcgc ataggtggag atgcgcgccc tgtcggccac atcggccagc gccagcggat
     4081 cggtggccgg cgcatagctg aggcccagcg ccaccacgct ggccgcctcg ggccacaggc
     4141 cctgcgggct gcggcggtgg tgcaggcgat cggccatcca gcccatctgg ccgtggcgcc
     4201 catcctccag ccaggcctcc agccgggcgg cgcgcgccgc atcctcggcc gccggcgcca
     4261 cgccaacggc ggcaaaaccc agcgcccgcg cttcggcgaa aagcgcctgc ttgaaatcgt
     4321 ttagggcagc gttaaccaag gctggcaatg atcctgttga gaggcttgcg gctagcgcag
     4381 gcaggtgaac cggcagcagg gcaaccggca ggcaaggggt gtggcaggtg gtgagcgcag
     4441 gagcaagcgc actggcgatc gaggcgcacg gcctcgtcaa gcggttcgac gggacggtgg
     4501 cggtggccgg cgtcgatctc gccgtgccgc aaggggcgat ctatggcgtg ctgggcccca
     4561 atggcgctgg caaaaccacc accttgcgca tgctgctggg cattatcgag cccgatgccg
     4621 gcacgcgccg ggtgctgggc gcggccaacc cgcaggcgat ggcccacgcc atcggctatc
     4681 tgcccgaaga gcgcgggctc tatcccacca tgaaggcctt cgaggcgatc gcctttctgg
     4741 gcgcgctgcg cggccagcca ctggccgaag ggcgccggcg ggcgctggaa ctgctggaag
     4801 cgcagggcct ttcccatgcg gcagaccggc agatccgcca cctttccaag ggcatggcgc
     4861 agcaggtgca attgctgggc acgctggtgc accgcccgca gctggtggtg ctggacgagc
     4921 ccttctccgg cctcgatgcg atcaaccagg ggcggctgga agcgatgatc cgcgatctgg
     4981 cgacaggcgg caccacggtg ctgttttcca cccatgtcat cgcccatgcc gagcgcatgt
     5041 gcgatgaggt ggcgatcatc gccggcggcc gggtgccctt tgccggcgct gtcgatgtgg
     5101 cgcgcgaccg gatcgccccg caggtgatgc tggaaacccg ccaccccgat ggcccctggc
     5161 gcgccgccct gccggccgat gccagggcag aaggccggcg ctggcatttc gccctgccgc
     5221 caagcggggt ggagcccctg ctgcgcgcgc tgatcgaagg ggaagccggc atcgtctcgc
     5281 tgtcgatcga gcgggcgggg ctgcatgatg ccttcatcgc cattgccggg gccgcggcgg
     5341 ccgatgccat ggccaccccc gcagcggagg aagcggcatg aacgcccccc acccccatca
     5401 cggccgcctg tcgctgctgg cggcggcgcg ggtgatcgcc cggcgcgatt ttatcgccat
     5461 cctgtggagc cgcagcttcc tcttcttcct gctggggccg ctgtttccgg cggtggtggg
     5521 cctgctggcg ggcaccgtgg gcagccaggc gaatgacagc atcggcccgc cggttatcgg
     5581 cgtgatgatg agccgggccg acaccgccgc catgctggcc gccgaggccg atctggcgcc
     5641 gcatctggcc gatgcgctgc cgcagctggt gccgctgggc gcgggcgcgc cggccagcca
     5701 tgcgcaagtg gccgcgatga tcagcggcac gccggcccat ccggtgctgg ccggccccgg
     5761 ccccgcgatc gaggcctggc agggcccggt ggcgctgctg gccgcgcgcg ccgatggcca
     5821 tgccgccatc cgctttgccc cggtgcgcct ggcccccacc gccaccagca ccgccgacac
     5881 cagccacgcc cgcctgctaa ccgcgcaagc cgcgcaaacc ctgctgctgc tgctgacgat
     5941 gctgctggcg ggcatggtgc tgtccaatct ggtggaggaa aaggccaaca agatcatcga
     6001 gatcctcgcc gccgccattc ccatggacgc ggtcttcctg ggcaagctgg tggccatgct
     6061 gggggtcagc ctggtgggca ttgcggtgtg gggggcggcg ggcgcgctgc tccatctggc
     6121 ggccgggcag gcgctgcccg cgctcaccgc gccggcggtg ggctggccgc tgttcgtggc
     6181 gctggggatt gcctatttcg ccatgggcta tctgctgctg ggctcggtgt ttctggcgat
     6241 cggctcgctg gccagcaccg tgcgcgaggt gcagaccctg tccatgccgg ccacgatgat
     6301 gcagatcctg gtgttcttct ttgccggcta tgccatcgcc cggccgggca gcggggcgga
     6361 actggcggcc atggtgttcc ccttcagctc gcccttcacc atggtggcgc gcgccgcgct
     6421 gcagccggcg ctgtggccgc atgtggcggc gctgggctgg caggcgctgt gggtggcgct
     6481 gctggtgcgc tggggggcgg cgctgttccg ccgccgggtg atgaaaagcg ggccgcaggc
     6541 agcgccgcgc cgccgccgct ggttcgcgcg cggctgaagg gctggcggcg ggcaggcgcg
     6601 gccgctaagg caggggccat tcgcgccaaa gggggggccc gcgccatgcc aacaccagtg
     6661 ccatcagggc cgcgccatgc ggcatgggcg cctgtcctgt ggctgatcct gctgctggcg
     6721 gcagggctgg gcgcggcgct ggtggattat cccatcgcca cgctctattt ccgcctgcag
     6781 gatcgctggc tggcgccgtt gagcgcgctg ctgctgctgg cgggtcttgt ggcgggctgg
     6841 gccgttatgc gcgggcgcgt ggcgccccgg cggccggggg cgcgttccat ggcggtgctg
     6901 gcgctggcgg tgctggcgct gggctgggcg ggccaccgct ggctgctggc cgattacgac
     6961 ctctcgcgcg atgagcagat ggccgtgttc gacgctttca tctacgccca tggccatctc
     7021 gtctggccgc tgccggcggc gtggcggggg gatgcgccgg cgctcaacct cttgttcatg
     7081 ctgccggtgg cgcggccggt ggcctgggtt tccacctatc tgccgatcca cgcgctgctg
     7141 cgggcgggga tgggccttgc aggcgcggca gcactcacct cgccgctgct gaacgccatc
     7201 gccgtgctgg ccacctgggg ggcggcgcgg cggctcttcg gcgcggcaga gcgcgagggc
     7261 gccggcttgg cggcggtgct gctggccaca tcgggccagt tcctgtttgc cggcatgagc
     7321 gcctatgcca tggccgccca tctggcctgc aacatggtgt ggctgtggct gttcctggcc
     7381 gatcggcggc gggccgatgg cgccgcgctg gcggtgggct tcctcgccac gggcctgcac
     7441 cagccgctgt tccacccgct gtttgtggcg cctttcctgc tgctgctggc ggcgcggcgg
     7501 cgctgggggc ggctggcgct gttcgtgctg gcctatctgc cgatcggcct gttctggctg
     7561 gcctggccgc atttcaccca tgatctggtg gcaggcacag gttctgtcag ccagagcggc
     7621 acctcctttg ccgagcggct gcgcgatgtg ctggccgcca acacccagaa cctgccgctg
     7681 atggccgaaa acctgctgcg ctttgccgcc tggcaggcgc cggtgtgggg gccgctggcg
     7741 ctgatcggcg gctgggcggc ctggcgccag cggcagggcg agatgctggc gctggccgca
     7801 gggctggtgg cgccggcgct gctgatggcc gcgctgctgc cgtttcaggg ccatggcttt
     7861 ggctatcgct atctgcatgg ggtgctgggc aatggcgcgc tgctggcggc ctggggctgg
     7921 gtggcgctgg gcgatcggcg ccagcgctgg cgcgcaggcc tggtggccgc gctggcggcc
     7981 ggcgcgctgg tgctgctgcc ggtggaagcg gggatggccc atgcgctgta tgcccccttt
     8041 gccgcggcca gcgcgcggct ggatgccagc ggcgccgatt atgtgctgat cggcgccgac
     8101 gaggccccgc tggcgctgga tctggtgcgc aaccggccgg atctggccaa ccgccccttg
     8161 cgcctctcca ccatcgacat cgacccgatg gagccgctgg ccgcccgcct gtgcggcgcc
     8221 gcgcccgcag gccatccgat gcgcgtgggc ctgcctgtgg acagcttcta tgccggcatc
     8281 aacgccgccc ttggcatgcc ccccgcccat gtggcagacc gccgcttcgc cgaggatgcc
     8341 gacgccttca gcgatgccgg ctgcacggtg gcggccctac gctgagccct tgcccaacag
     8401 ccgccgcatg atcgccagcc gggcgttggc ataattgtag accatcatca gcagggtgga
     8461 agccggggcg gcaaaccaca tcggcaggcg caacaggctg acaaacagcg ccaacagcgc
     8521 cagcgacagg ggcaccccca gcgccacccc gcccatgaag cgcagatagg cccggccccg
     8581 ccacggctgg cggaaggtga agcgcgcgtt cagcccatag cccagagagc ccgccagcgc
     8641 ccagctgatc gccgtcagca gcgcataatg gaggcccagc ccatccccgc cgatgaggat
     8701 gatgttgttc accaccgcgc acaggccgcc acacagcaga tagcgcagcg gctcgcggtc
     8761 gatcagcgcg cggatgcgcg gggccagtgc cgtctcgcgg ctcacagctt gcccgccagc
     8821 atcagcttca cccgcggcca cagccggccg atgatctgcg tttcctggca gaagcgccag
     8881 gcgcccagcg gccccagccg cgccacctgc cccagcgccc ccatcagcgc cgaacgatcg
     8941 ccggcagaaa cccccgccag cgcgacattc aacgccacag acgcgcgcgc ccgggccagg
     9001 gcggcggccg gcggcgccag ctgcggcgca tgggcatcga gcacccgctc catctgctgg
     9061 cggaaatcgg ccagatcccg gctaccggtc atcgtcagcg agccgccgtg gaggcgaaag
     9121 gccgtggtcg cccctgcccg cacgaacaca tcgccgcggc gcgccagctt cagccacagg
     9181 tcccagtcgg ccgtatacca cagcgcatca tccatgccgc cggccgccag aaaggcatcg
     9241 cggcggaaca gcggcgccgg caccgcgatg gtgttctggg tgagcagcct tgtggccaca
     9301 tcaccgccct gctggcgccc ctgccgcagc ggcaggcgcc acaggccgac atcctgcccc
     9361 gccggcccca ccagccgcga tggccccacc gacagcaccg catcgcccgc cagcgccagc
     9421 gaggcccgca acgccgccag atggcccggc agccacagat catcctgatg gaggatagcg
     9481 atatggggcg cgctggcatg gcccacgccc aggtttacct tggccgtcca cgggcggata
     9541 tcgggcacat cggcccagcg gatcgccagc tggtcggcat gggcttcggc aatggcgcgg
     9601 gcctggccgc catccgcgcc cgaatcatag cagaggaatt ccaccccctg cggcgcctcg
     9661 gcggccacgc tggccagcgt tgcgcccaga taggtggccc cgccatggat cggcatcacc
     9721 acgctcagcc atggctggcc ctgcagcggc aggctcatcc ctg
//
LOCUS       DASRYE010000015        27901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_35778,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000015 DASRYE010000000
VERSION     DASRYE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 27901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27901
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35778"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFF98_00695"
     CDS             <1..856
                     /locus_tag="VFF98_00695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62171.1"
                     /translation="TRAIIAHYAAADARIRPIFRENKGLIASLNQLIAEARAPLIARM
                     DADDICHPDRFARQHAFLAAHPDHGVIGTRTEDMDEHGRLYPPCANPHPLTHADFLAA
                     IAAEGPLLCHPSVMMRRDVVLAVGGYHAAFHHCEDLDLWLRLASLTRIANLPEPLLRY
                     RHYADQVSSRHATQQQVGAAVARLAYQERAEGRPDPTATLAELPPVDALDALFGRPGV
                     SAAVRAKVAPGLLYSLSALRGAGFDIILRYLAEGGRHAGMWRTVLRLLRFGEATRALR
                     LAAALTAR"
     gene            868..2022
                     /locus_tag="VFF98_00700"
     CDS             868..2022
                     /locus_tag="VFF98_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62172.1"
                     /translation="MPTPQIALSSLALPADGPHLRQPPRPVDLRGPNYAARFDATTLF
                     YDVYRAGAHVVLQGPPFLNLQALLAQSAPLAGALAGWWPRARIRPRFKAGEIWWRNGP
                     DHFTLSGPLGTHAIAVQPDGAALFAGRRVIHTLSKDNDIAWIIDWARFYQRHHGADAV
                     LLYDNGSSAYDAAELEETLATACPQMLVRVVSWSFPYGPQGGLAAGIPGIEAPWDSDF
                     CQTGSMQHARHRFLRHARSVLNADIDELVLGPAGQSIFAAAEASRAGFVKFAGRWISA
                     ARPDAAAHTPPRHGHFLWQDSEEAETCPPKWCLVPRPWAHRRCSWSVHNLFGARANRR
                     ISPAFTYRHFKGISTSWKYDRQESTPFTPARFTPDDTLAAALAAADLIPG"
     gene            2068..3894
                     /locus_tag="VFF98_00705"
     CDS             2068..3894
                     /locus_tag="VFF98_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZU62173.1"
                     /translation="MPPETPAAPGSRPARHDGWRTLHRFLPYLWPEGNPALRLRIMVA
                     MVLILAAKVTTLSLPYAYKRAVDAMGVGGPAAGQVALAFILAYAGGRFAGIAFDNLRN
                     IVFERVGQDATRALAEDVFARLHQLSLRFHLARRTGEVTKVIERGTKSIDTMLYFLLF
                     NIAPTVLELAAVAVIFYLHFGPAMVVATALAVALYGLATRWITEWRSHLREKMNRLDG
                     QALARAVDSLLNYETVKYFTAETRERERYAAATRAYADAAVVSENSLGLLNIAQALVV
                     NLLMAAAMGYTVWGWAQGRYTLGDLVFVNTYLTQLFRPLDLLGMVYRTIRQGLIDMAS
                     MFALIDTEVEVRDAPAAPALVVRQPSVVFEEVAFGYEPERQILKGLSFEVPAGAHVAI
                     VGPSGAGKSTIGRLLFRFYDPQAGRILVDGQDIRSVTQASLRAILGIVPQDSVLFNDT
                     LGYNIGYGRDGASQAEIEEAARAAALTGLIERLPKRYATEVGERGLKLSGGEKQRVAI
                     ARTLLKNPRILLLDEATSALDTRTEQDILATLHTLSANRTSIAIAHRLSTIADADRIF
                     VVNDGLLAEAGTHTELLRADGLYADMWARQANEAATLDEAAQ"
     gene            complement(3964..4755)
                     /locus_tag="VFF98_00710"
     CDS             complement(3964..4755)
                     /locus_tag="VFF98_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HZU62174.1"
                     /translation="MKIKFVCAAAAVAFACSSQVAFAEDAPTPEITVTGSAAVVSQYR
                     FRGISQSDNKPAVQAAITVADKSGFYVSFWGSSASAGQSQVNIGGTEIDVYGGYTHAI
                     GKTGITFDGGLYGYLYPGAPAGNYFELYGSLSKAYGPFTVKGGLNWAPDQGYFDAYQG
                     ISHYNMYEYGEISFSPAKLPALTIHTHLGHTGGGFNYTKEYLDYTVGASYKWKNLTLD
                     ASVVGTNVSSDNASNAGFIGSGPGSAYEIYRAAKTAGVVSLTASF"
     gene            complement(4946..5140)
                     /locus_tag="VFF98_00715"
     CDS             complement(4946..5140)
                     /locus_tag="VFF98_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HZU62175.1"
                     /translation="MTMPPDERPVYRLLTGKDDRAFCERVSEALAQGWRLYGSPAMTY
                     DAENACVKVAQAVVWKDWAV"
     gene            complement(5219..6520)
                     /gene="hslU"
                     /locus_tag="VFF98_00720"
     CDS             complement(5219..6520)
                     /gene="hslU"
                     /locus_tag="VFF98_00720"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZU62176.1"
                     /translation="MNDNLTPKAIVAALDAHIVGQAEAKKAVAVALRNRWRRQHLPAA
                     LREEVSPKNILMIGPTGCGKTEISRRLARLADAPFVKVEATKFTEVGYVGRDVEQIGR
                     DLAEEAIRLEKARRREAVREAAARAAQERLLKALVGDSASEATRESFRQRLAEGAMAD
                     VEVEIEVEDTPSAPMEIPGMGGGMGMINLGDMMAKAMGRHQMKRRKMKVADALDKLID
                     EEAEKRMDQDDIARVALADAEANGIVFIDEIDKIAVSDVRGGSVSREGVQRDLLPLIE
                     GTTIATKYGPMKTDHVLFIASGAFHVAKPSDMLPELQGRLPIRVELTALSEADFVRIL
                     SDTRANLVEQYRALLATEEVAVEITPDAVAEIARIAAQVNETVENIGARRLQTVMEKV
                     LEELSFEAEERRGAQVTIDAAYVRQRLDGLASDADLSKYIL"
     gene            complement(6529..7104)
                     /gene="hslV"
                     /locus_tag="VFF98_00725"
     CDS             complement(6529..7104)
                     /gene="hslV"
                     /locus_tag="VFF98_00725"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZU62177.1"
                     /translation="MESTKAGHGLIQWHGTTIIGVRRGGKTVIAGDGQVSMGNTVMKP
                     NARKVRRLGAEGKVIGGFAGATADAFTLFERLERKLEAHRGQLLRAAVELAKDWRTDK
                     YLRNLEALMIVADAESLLVLTGNGDVLEPEAQGDTMIAAIGSGGNYALAAARALADYE
                     TDPEKIARRAMAVAAELCVFTNDRVTVETIG"
     gene            complement(7224..7742)
                     /gene="bamE"
                     /locus_tag="VFF98_00730"
     CDS             complement(7224..7742)
                     /gene="bamE"
                     /locus_tag="VFF98_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZU62178.1"
                     /translation="MQVRFSMRMKLAALAGMALAAGGCATIHDHRGYVIDQALLDSVQ
                     PGVDNRLSVEHTLGRPTFTSQFGTPVWYYVSVDTAQPVARRPHPVKETVLRLTFDDKG
                     NVARVDRAGLDKVVTLKPEKSETPTLGRQRTFLEDLFGNIGTVGAAGLGSGGSGPGGG
                     GGGGGGGGPNGS"
     gene            7927..8493
                     /locus_tag="VFF98_00735"
     CDS             7927..8493
                     /locus_tag="VFF98_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HZU62179.1"
                     /translation="MNHTATATAPAPAGFLSRLLGGLFSRRADPREALRPLWHGVVAS
                     ARRPDWYTSCGIADSLPGRFDAITLVLALVLLRLETEAGQTEAGVRLTELFVEDMDGQ
                     LREAGVGDLVVGKHIGRLMSVLGGRLGAYRAGLAEADDAELHAALTRNVTLTEGSAPQ
                     ALAAATRQLATALAALPAERLLAGEMPA"
     gene            8490..9023
                     /locus_tag="VFF98_00740"
     CDS             8490..9023
                     /locus_tag="VFF98_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZU62180.1"
                     /translation="MSAPEATGEFSRIVDLRQFDQAPTHIAASTAECAALARRFGLVR
                     VEQLEADVACTAEGASVRVTGRVRAHIVQSCAVSAEDLPVTIDEPIAFRFVPSGTPLL
                     ATDEEGIEITAEDCDEIAFDQGCFDLGEAVAQSMALAIDPFATGPEADQVRASGLLGA
                     ENTSPFAALAALTKKED"
     gene            complement(9064..9654)
                     /gene="ssb"
                     /locus_tag="VFF98_00745"
     CDS             complement(9064..9654)
                     /gene="ssb"
                     /locus_tag="VFF98_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZU62181.1"
                     /translation="MAGSVNKVILVGNLGADPEVKSFQSGGRIANLRIATSESWKDRS
                     TGERKERTEWHSVVLQSDGLVGVAERFLRKGSKVYIEGQLRTRKWQDQNGNDRYTTEI
                     SVGGVGGVLTMLDGAPGGGQGGGGRGGDFGGSSGGGYGGGQRGGGSSSGGGDWGRSGG
                     SPGGASGGGSSGGSDWGRTGGSGGGFAEDLDDDIPF"
     gene            complement(9669..11519)
                     /locus_tag="VFF98_00750"
     CDS             complement(9669..11519)
                     /locus_tag="VFF98_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZU62182.1"
                     /translation="MSRRRSAALVGNPNAGKSALFNALTGARQKIANYPGVTVERKGG
                     RMVLPSGEPIELTDLPGSYGLDATSPDEEVTRRVILGELPGEAAPDVLVVVLDASNLE
                     QHLVFAQEVIALGKPVVVALNMVDLAERDGLVIDPGALEGALGVPVIPTVAVRRRGLG
                     ELGEAIARARPAGTRAHLDMAERRLEAHAIADAAILSETRRHRLHARMDRLLLHPWIG
                     PIILFATLFIVFQAVFSGAKPLQDALQAGVDAIGALVRAELPASLWRDLLIDGVLAGV
                     GAVVVFLPQIIILFFFILSLEASGYMARAAFLMDRMMAGVGLSGRSFIPLLSSFACAV
                     PGIMATRSITDPRDRLTTILIAPLMTCSARLPVYAVIIAAFIPNRPLGAVGTLAGLIG
                     LQGLVLFALYVAGIVGAMVVALLLRATLTKGAASGFIMELPKYQLPRAKDLALGLMQR
                     AWIFLRRAGTIIFQVSVVLWLLLNFPRAEPGKDQIDASLAGHIANGLAVVVKPIGFNR
                     DIALSLIPSMAAREVAVSSLATTYAVASSDEEVTGKALAGQLQKHWSLATALAFLAWF
                     VFAPQCMSTIAVTRRETNGWKWPAFMLAYLFGLAYLFAGITYWSAVALGL"
     gene            complement(11516..11839)
                     /locus_tag="VFF98_00755"
     CDS             complement(11516..11839)
                     /locus_tag="VFF98_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZU62183.1"
                     /translation="MALDRLPLRCPARIVAVDWAALVPEEAKRLRALGLDVGARIRVA
                     HKGVFFAADPLAISVGRMIVALRRVHARAMQVEPLPARGPHHHRGRHHGHGGPHHGRH
                     EEQGA"
     gene            complement(11980..12672)
                     /locus_tag="VFF98_00760"
     CDS             complement(11980..12672)
                     /locus_tag="VFF98_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HZU62184.1"
                     /translation="MSAPQTLSPESLPPLEERSLEDLRIALAPALADAAAFDGWSMEA
                     VRAAAARLGVNPDVAAYAFQGGAMAMIAAFTAHVDAAMAAACPVEAMAALSVRGRIAG
                     LIRARLAEQQGREEALRRALAIMALPQNIAAALKLGWVSADAMWRQAGDTAADYNHYT
                     KRATLAAVYAATLAVFVDDKSEGKADTLAFLDRRIAGILRFEKAKARFTPPERVPFSL
                     VRLAGRLRYPAR"
     gene            complement(12669..13298)
                     /locus_tag="VFF98_00765"
     CDS             complement(12669..13298)
                     /locus_tag="VFF98_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZU62185.1"
                     /translation="MLKFARFFRGVPVRAGLALVLAAGLVAPAEAQFSKAYKFLDAVR
                     KKDGDEVNDDLSQPGTTLINTHDSTSGETALHIVTGRRDSTWMRFLIGKGAEVNTHDN
                     RGVTPLQLASNIGFAEGVDILLQNGARVDETDSLGDTPLMAAVQRRDIAMMRVLLKAG
                     ANPDRADNTGRSAREYARENDRSGNLLEEIESYSKQAKVRSTAGYGPHL"
     gene            13397..14029
                     /locus_tag="VFF98_00770"
     CDS             13397..14029
                     /locus_tag="VFF98_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZU62186.1"
                     /translation="MTKPSPFPLARPARRALAALSLAGLALLGACHAKPAADAAGDAP
                     LAGAAIGGDFTLVDKAGHTVRWADFAGHYRIVYFGYTFCPDACPTDLNVAMRGLDRFA
                     KAHPQAADRIRPIFITIDPARDTPAAVGQFAAAFSPRLLGLTGTPDQVAAAAKAFAVY
                     YARGKDTPGGYLMDHSRIAYLFGPKGEAIDMLPVDKGPDAVAQDLARWVK"
     gene            14026..14508
                     /locus_tag="VFF98_00775"
     CDS             14026..14508
                     /locus_tag="VFF98_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HZU62187.1"
                     /translation="MSGPVPFWERPVHTLSREEWEALCDGCGQCCLHKVEDADTGDIY
                     PTNVACKLLDIPSGRCSDYAGRRRQVPDCVRLTPRLAATLPWLPDSCAYRLRAAGEPL
                     PAWHYLVCGDREAVHAAGASVRGKAISEVLAGPLEQHIVWPGEPMEVVAVEDAPSLDD
                     "
     gene            14514..15245
                     /locus_tag="VFF98_00780"
     CDS             14514..15245
                     /locus_tag="VFF98_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZU62188.1"
                     /translation="MRWPRPRPPAAQAPHLELAGQRLPVEIRKLAQARRITLRLARDG
                     SAVRISMPRWAAEAEALRFALSRREWLAAQLARLPRPADPQAPLTHIAWRGERLALQH
                     DPAAPRRIRAAPPALHIGGPADSLKPRLQRWLKAEALRLLAADLAEYCARAGVAQPAL
                     ALSSAQARWGSCSTRGVVRINWRLVMAPDGVRRSVVAHEVAHLVHFDHSPAFHALLAS
                     LFGADLPACDRWLKAHGRTLYLPFG"
     gene            15320..15784
                     /locus_tag="VFF98_00785"
     CDS             15320..15784
                     /locus_tag="VFF98_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62189.1"
                     /translation="MLFKRKGQFWDDVSLSGFIGDFLTVFRQAGRLRWRISALAVACT
                     YAVFSLIIHDEHRIEPRLPTITYITSWEDGRTDAEILAGNRANQRRKEAEAAREAAAD
                     ADVKGMYKAVGRASGLDVDQMERQAKADAAKDAAAAKAKQAALYGAGHTATP"
     gene            15786..16784
                     /gene="ribD"
                     /locus_tag="VFF98_00790"
     CDS             15786..16784
                     /gene="ribD"
                     /locus_tag="VFF98_00790"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZU62190.1"
                     /translation="MASSPPSATDARWLAATARLAMRARPLSRPNPGVAALIVKDGRV
                     IARGWTAPGGRPHAEAVALAAAGARAAGATLYVTLEPCAHDSARGPACADLVAASPLA
                     RVVAGVMDPDPRTAGAGLARIRAAGKQALHLPCPAARASLEGYLMQRLAGRPFVTLKL
                     ATSLDGCIATAAGESQWITGAAARAHTHALRAAADAILVGGGTWRADAPRLDVRLAGL
                     ESRSPARWVLTHGPVGAGWQALAAPEAIHTAMPDVQYLFVEGGAGAAAAFLGAGLVDR
                     VLLYRAPLFIGGGKPCLGDVGLGPLADAHGQWRHADHRQLGKDTLDIYERAPCSPA"
     gene            16769..17386
                     /locus_tag="VFF98_00795"
     CDS             16769..17386
                     /locus_tag="VFF98_00795"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZU62191.1"
                     /translation="MFTGIITAIGTIASVRASGDLHATITSPYPAGGIAIGASIACSG
                     VCLTVVEKGERAGGSWFAVDISGETVARSVPGRWAEGAQLNLEQALKLGEELGGHIVT
                     GHVDGVGQVVAIEPVGESLKFTIAAPAELAPYIAPKGSITVDGVSLTVNDVTDEADGT
                     ARFTLNIIPHTAEVTTIGQLVAGAGVNLEIDVLARYLKRMQALKG"
     gene            complement(17442..17987)
                     /locus_tag="VFF98_00800"
     CDS             complement(17442..17987)
                     /locus_tag="VFF98_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU62192.1"
                     /translation="MERQPVLEGGRLALRPLAPEDWDALYAVASDPLIWEQHPAHDRW
                     REEVFRAFFADALASGGAIVAVDAATGAVIGSSRWDAYDPAGGGAVEIGWTFLARSAW
                     GTGANAAMKRLMLAHAFRYVARVDFRIGEANRRSRIAAERIGARLTERSDVAMMGGQA
                     VRHVIYALERTAFARGLAAKG"
     gene            complement(17996..19174)
                     /locus_tag="VFF98_00805"
     CDS             complement(17996..19174)
                     /locus_tag="VFF98_00805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZU62193.1"
                     /translation="MLTNLQQQPADALLALIKLHNADPREDKIDLGVGVYRTDTGATP
                     VFAAVKAAEKKLLEIQDSKAYLGPEGDMGFVHALMPYIFGQANPTLDGRIEGMQAPGG
                     TGAVRLAVALAKRAGIQRIWMGTPSWPNHAQIAADVGVAIKGFAHATKAGVADMDALR
                     TAIASAEAGDAIMLHGCCHNPTGIDYTPAEWDEIAALIKAKGILPILDLAYQGLGQGM
                     EEDAYGVRRVLAAVPEALIAYSCDKNFGYYRDRVGALYVLAAEASQIGAILSNGHALA
                     RANWSMPPDHGGAVVRLILEDAELTASWLNELDVMRARMNEVRARLGGAGIQGSVDFA
                     PFGTQNGLFSIVPLSPEQVLAMREKHGVYMAGSGRINVAGLTMGNIDKFIAAVADVTA
                     "
     gene            complement(19236..20135)
                     /locus_tag="VFF98_00810"
     CDS             complement(19236..20135)
                     /locus_tag="VFF98_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HZU62194.1"
                     /translation="MSKHSNLHGAASGRAGRLTGLLARRGLAHRGLARAALVAGALGL
                     AVGPALATAAAAQQSYGQQSYGQQAYGAQSGGVDSDLMPTPQASATASPRPAYTGAPS
                     GTAAAPYAGGSGAYQGDPTAAPRYNGYNGAGEPNNRNGTYHEDDLIGAATGVFGKGAH
                     GLADVIKDILKKQGEPNGYIVGREAGGALAVGLRYGSGTLFHKVEGQMPVYWTGPSLG
                     FDVGASAAKTFVLVYNLYNTDDLFHRYGAGEGQAYFIGGFHVSYLRRGDVVLIPVRLG
                     VGLRLGINGGYMKMSRHQNWLPF"
     gene            20396..20487
                     /locus_tag="VFF98_00815"
     tRNA            20396..20487
                     /locus_tag="VFF98_00815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20430..20432,aa:Ser,seq:gct)
     gene            complement(20558..21343)
                     /locus_tag="VFF98_00820"
     CDS             complement(20558..21343)
                     /locus_tag="VFF98_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZU62195.1"
                     /translation="MHNSFKSRVAGQPLFGLWLALADQYCAEVCAGAGFDFLLLDGEH
                     SPVDLRIILGQLQAIAAYDVAPVVRPPTDDPVLIKQLLDIGANNLLIPMVESGAQARR
                     IVASTRYPPEGFRGVGSGIARAARWGNDPSYMATANENICLILQVESVAGLNNLEDIA
                     ATAGVDGVFFGAVDLSASMGLLGKPDHPDVIASIDAAIARVRALGKFAGFLSASPGVS
                     QHFLATGAHFAAVGVDALLLSNAARALVHGFRAKGDPFVPGGY"
     gene            complement(21355..22836)
                     /locus_tag="VFF98_00825"
     CDS             complement(21355..22836)
                     /locus_tag="VFF98_00825"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HZU62196.1"
                     /translation="MPDTNNARRDAAHFIDGAFTCGQSGKSWTNHSPIDGSVIGLVPE
                     GGEAEVAAAVAAAQAALAGPWGRMSVAERTDLLGAVADGITARFNDFLEAECLDTGKP
                     FALASHIDIPRGAANFRLFTDIAKTVATECFSTPTPDGQGALNYGLRRPRGVIAVISP
                     WNLPLLLMTWKVGPALALGNTVVVKPSEDTPLTATLLGEVMQAAGVPRGVYNVVHGLG
                     PASAGEYLTRHPGINGITFTGETRTGAAIMQQAAQGIRPVSFELGGKNPAIIFADADL
                     DKAVEGTMRSVFANCGQVCLGTERVYVERPLFDAFVARMKAGAEGLRPGRPEDATTTL
                     GPLVSAEHKAKVLSYYARAVEQGAQVITGGGVPDMPAALAGGAWVQPTIWTGLADDAD
                     CVTQEIFGPCCHIRPFDTEEEAIRLANASPYGLAAAVWTENLARAHRVAARMEVGICW
                     VNAWFLRDLRTAFGGAKQSGIGREGGVHGLEFYTELTNVCVKL"
     gene            complement(22829..23257)
                     /locus_tag="VFF98_00830"
     CDS             complement(22829..23257)
                     /locus_tag="VFF98_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZU62197.1"
                     /translation="MALSFTHAAITLDAAEAILARAMARSRAMGLRLAIAICDPAGHL
                     VALRRDDGTWPPSIDVAKDKAATSAIFGLSTDDLGAALSYSPALHQGLSQRPGVVLFG
                     GGLPILLGEAVIGGIGASGASEQEERACLTYALEHWTDYA"
     gene            complement(23269..24189)
                     /locus_tag="VFF98_00835"
     CDS             complement(23269..24189)
                     /locus_tag="VFF98_00835"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HZU62198.1"
                     /translation="MAMKGILRPGLVQIRVMDVEAALSHYVDRLGLYKVGSDAGRAFL
                     KGADEFEHHSVTLVEADTPGMDFIAFKVMEEADLDHFEARVRAEGIAVAHIAAGEQPG
                     YGRRISFVAPSGHTIQLFAHAEQSEPKPDLANPDIWPVEPHGMGARRFDHMQLNGVRV
                     PETLDFFTRVLDFYLAEKVDDESGAPIAIWLTCGMKAHDVAFVDGGVPGRMHHAAFEL
                     ADWNAVGHAADIITKYNLSIDLGPTRHGITRGRTIYFFDPSGNRTEVFAEGYTYYPDH
                     PVRVWDKQSLGKGIFYYQRQLNDNFMNVVT"
     gene            complement(24318..25322)
                     /locus_tag="VFF98_00840"
     CDS             complement(24318..25322)
                     /locus_tag="VFF98_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZU62199.1"
                     /translation="MTQTTNLPARPPRDLAMARVAAAAAVAALASVPLWGDASAERIL
                     GEFLVYAALATLWNLLASYGGLLSVGQQAFIGIGGFALYGFTVLAGVPMLAALPLVAL
                     VGLVAAVPMGLLSLRLSGPYFAIGTWAIAEILRLTVLQFPSLGGGSGASLPSDVLHTL
                     GETRAVRSRVVYAVAFAVAAGSVGITFALLRSRWGLGLRALRDSGIAAEAVGVRVKPL
                     KIALFCVVGVMTSLVGAVVVMQKLRVAPDPQFAVVDWTAFVIFMVVIGGYGTIEGPLL
                     GAALFILLRELLADFGTVYQIVLGAIAIVMMVAARKGVWGLARGASRFPLLSVVRRSA
                     "
     gene            complement(25325..26191)
                     /locus_tag="VFF98_00845"
     CDS             complement(25325..26191)
                     /locus_tag="VFF98_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZU62200.1"
                     /translation="MIWVNALVQGLLLGGIYALAASGLALVFGVMRIINMAHGDIIVV
                     AAFLAQVLAVGLGVSPFVVAVPVVLIVAAAGYWLQRLLVNRALGHDPLPALIMTFGLS
                     IILQNVLLSLFSADSRRLDAGPLADLSLAVMPGVAVGVLPLAMFGVAVLALVGLQWVF
                     FHTAAGRAFRASADDAEIARVMGLDTNHVYALVTSIAFGLAALSGIFMGVYGSFDPFI
                     GPSRLIFAFEVIVIGGMGSIYGALMGGILLGVSQALGAQINPQFQQLAGHLVFLAVIV
                     LHPQGVLARSKA"
     gene            complement(26188..26898)
                     /locus_tag="VFF98_00850"
     CDS             complement(26188..26898)
                     /locus_tag="VFF98_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62201.1"
                     /translation="MTEPRLSVSGLSSFYGGFQALFDVSFSIAPGAVLALIGANGAGK
                     TTLLKTLAGVLRPPARSIHHNGACLASRTPAETTRAGVAVVPEGRRLFASLSVEENLL
                     IGAQVKRPGPWTLEAVYALFPALVQRRHHLGPQLSGGQQQMVAIGRALMANPDLLLLD
                     ELSLGLAPVVIEEIYRAFRQIVGPGVSAILVEQDINRALSVSDQFLCLRAGKVVLSGD
                     SRTADQAAIIDAYFGVHA"
     gene            complement(26891..27616)
                     /locus_tag="VFF98_00855"
     CDS             complement(26891..27616)
                     /locus_tag="VFF98_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62202.1"
                     /translation="MSALLELSGLSRSFGALKVTDDVSLAIPEGQAVGIIGPNGAGKS
                     TLFSLITGTLMPDAGQVGFVGQDVSRRPAAWRSRHGIARSFQVPQPFAGMTVFENLLT
                     AASFGGGLAGRAAAQRAHAVLAQTHLAHRADTLAGDLTLLDRKRVELARALATNPRLL
                     LLDEIAGGLTDAECAQLIDIVRDIHAGGVTIVWIEHIVHALMAVVSRLVVLDTGRVVA
                     DGPPQAVISSPAVVALYTGMAHD"
     gene            complement(27613..>27901)
                     /locus_tag="VFF98_00860"
     CDS             complement(27613..>27901)
                     /locus_tag="VFF98_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62203.1"
                     /translation="PPLGFKHALFEVAVDVLRRTTALEPEAIRDAIKTTSLDTIAGHV
                     DWARGPVANVSTTPLVGGQWLAGGRFGVEQKIVFDKDAPAIPVGANLALLS"
BASE COUNT         4622 a        10162 c         8815 g         4302 t
ORIGIN      
        1 cacccgcgcc atcatcgccc attatgccgc tgccgacgcc cgcatccgcc ccatcttccg
       61 cgagaacaag ggcctcatcg ccagcctcaa ccagctgatc gccgaagcgc gcgcgccgct
      121 gatcgcccgg atggacgccg acgacatctg ccacccggat cgtttcgcca ggcagcacgc
      181 cttccttgcc gctcaccccg atcacggcgt tatcggcacc cgcaccgagg atatggacga
      241 gcacggccgc ctctatccgc cttgcgccaa tccccatccc ctcacccatg ccgatttcct
      301 ggccgccatt gcggctgaag ggccgctatt gtgccacccc tcggtgatga tgcggcgcga
      361 tgtggtgctg gccgtcggcg gctatcacgc cgccttccac cattgcgagg atctcgacct
      421 gtggctgcgc ctggccagcc tcacccgcat cgccaacctg cccgagccgc tgctgcgcta
      481 tcgccactat gccgatcagg tgtccagccg ccatgccacg cagcagcagg tgggcgcggc
      541 cgtggcgcgg cttgcctatc aggaacgcgc cgaaggccgc cccgatccca ccgccaccct
      601 ggccgaactg cccccggtag acgcgctcga tgccctgttc ggccgcccgg gcgtaagcgc
      661 ggccgtgcgc gccaaggtag cgccgggcct gctctattcg ctcagcgccc tgcgcggggc
      721 gggcttcgat atcatcctgc gctatcttgc cgaaggcggg cgccacgccg gcatgtggcg
      781 cacggtgctg cgcctgctgc gctttggcga ggccacccgc gccctgcgcc tggccgctgc
      841 ccttaccgcc cgctgatcgc gccgcccatg cccacgcccc agatcgccct cagcagcctt
      901 gccctgccgg cagatggccc gcatctgcgc cagccccccc ggccggtgga tttgcgcggc
      961 cccaactatg ccgcccgctt cgatgccacc accctgttct acgatgtcta tcgcgccggc
     1021 gcccatgtcg tgctgcaggg cccacccttc ctcaacctac aggccctgct ggcgcaatcc
     1081 gccccgctgg ccggtgcgct ggccggctgg tggccgcgcg cgcgcatccg cccccgcttc
     1141 aaggccggcg agatctggtg gcgcaacggg cccgatcatt tcaccctctc cggcccgctg
     1201 ggcacccacg ccatcgccgt gcagcccgat ggcgccgcgc ttttcgccgg ccgccgggtg
     1261 atccacaccc tgtcgaaaga caatgacatc gcctggatca tcgactgggc ccgcttctac
     1321 cagcgccacc acggcgccga tgccgtgctg ctctacgaca atggctccag cgcctatgac
     1381 gccgcagagc tggaagagac gctggccacc gcctgcccgc agatgctggt gcgcgttgtc
     1441 tcctggtcct tcccctatgg cccgcaaggg ggactggcag ccggcatccc cggcatcgag
     1501 gcgccctggg attccgactt ctgccagacc ggctcgatgc agcacgcccg ccaccgcttt
     1561 ttgcgccacg cccgcagcgt gctcaacgcc gatatcgacg agctggtgct gggccctgcc
     1621 ggccagtcga tcttcgccgc cgccgaagcg agccgcgccg gcttcgtcaa attcgccggg
     1681 cgctggatca gcgccgcccg ccccgatgcc gcggcccaca ccccgccccg ccacggccac
     1741 ttcctgtggc aggatagcga agaggccgaa acctgcccgc ccaaatggtg cctggtgccg
     1801 cgcccctggg cgcatcggcg ctgcagctgg tcggtgcaca atctcttcgg cgcgcgggcc
     1861 aaccggcgga tctcccccgc cttcacctat cgccacttca agggcatctc caccagctgg
     1921 aaatacgacc ggcaggaaag cacccccttc acccccgccc gcttcacgcc agacgacacg
     1981 ctggccgcag ccctcgccgc agcggatctg atccccggct gatcgcccgc gattggtttt
     2041 tgccgccgct gcccctacat ggggcccatg ccgcccgaaa cgcccgccgc ccccggttcc
     2101 cgccctgccc gccacgatgg ctggcgcacg cttcaccgct tcctgcccta tctgtggcca
     2161 gaggggaatc cggcgctgcg cctgcgcatc atggtcgcca tggtgctgat cctggcggcc
     2221 aaggtcacca ccctcagcct gccctatgcc tacaagcgcg cggtcgatgc gatgggggtg
     2281 ggcgggccgg ccgcggggca ggtggcgctg gccttcatcc tggcctatgc gggcggccgc
     2341 tttgccggca tcgccttcga caatctgcgc aacatcgtct tcgagcgcgt tggccaggat
     2401 gccaccaggg ccctggccga ggatgtgttt gcccggctgc accagctctc gctgcgcttc
     2461 cacctggccc gccgcaccgg cgaggtcacc aaggtgatcg agcgcggcac caagagcatc
     2521 gacacgatgc tctatttcct gctcttcaac atcgccccca cggtgctgga actggccgcc
     2581 gtggcggtga tcttctatct ccatttcggc ccggcgatgg tggtggccac ggcccttgcg
     2641 gtggcgctct atggcctggc cacgcgctgg atcaccgaat ggcgctcgca cctgcgcgag
     2701 aagatgaacc ggctcgacgg gcaggcgctg gcccgcgcgg tcgattcgct gctgaactac
     2761 gagacggtga agtatttcac cgccgagacg cgtgagcgcg aacgctatgc cgccgccacc
     2821 cgcgcctatg ccgatgcggc ggttgtgtcg gaaaactcgc tgggcctgct gaacatcgcc
     2881 caggcgctgg tggttaacct gctgatggcc gccgccatgg gctacaccgt gtggggctgg
     2941 gcccagggcc gctacacgct gggcgatctc gtcttcgtca acacctatct cacccagctg
     3001 ttccgcccgc tcgatctgct cggcatggtc tatcgcacca tccgccaggg gctgatcgac
     3061 atggccagca tgttcgccct gatcgacacc gaggtggaag tgcgcgatgc gccggcagcg
     3121 cccgcgctgg tggtgcgcca gccctcggtg gtcttcgagg aagtcgcctt cggctacgag
     3181 cccgagcggc agatcctcaa gggcctctcc ttcgaagtgc cggccggcgc ccatgtggcg
     3241 atcgtcggcc cctcgggcgc gggcaagagc acgatcggcc gcctgctgtt ccgcttctac
     3301 gatccgcagg ccggccgcat cctcgtcgac gggcaggata tccgcagcgt cacccaggcc
     3361 tccttgcggg ccatcctggg cattgtgccg caggattcgg tgctgttcaa cgacacgctg
     3421 ggctacaaca tcggctatgg ccgcgatggc gccagccagg ccgagatcga ggaagccgcc
     3481 cgcgcggccg ctctcaccgg cctgatcgaa cgcctgccca agcgctatgc caccgaagtg
     3541 ggcgagcgcg gcctcaagct ttcgggcggc gagaagcagc gcgtggcgat cgcccgcacc
     3601 ctgctcaaga acccgcgcat cctgctgctc gacgaggcca ccagcgcgct cgacacccgc
     3661 accgagcagg atatcctcgc cactctccac acgctttcgg ccaaccgcac cagcatcgcc
     3721 atcgcccacc gcctgtccac catcgccgat gccgatcgca tcttcgtggt gaacgatggc
     3781 ctgctggcag aagcaggcac ccacaccgaa ctgctgcgcg ccgacgggct ctatgccgac
     3841 atgtgggccc gccaggccaa tgaagccgcc acgctggacg aggccgccca ataagcgaaa
     3901 gggccgggct gcccccctgg caacccggcc ccttccttga gaggttcgct gtaaagcgca
     3961 gccttagaag ctggcggtca gcgacaccac gccggcggtc ttggcggcgc gatagatttc
     4021 ataggccgaa cccgggcccg aaccgatgaa gccggcattg ctggcgttat ccgacgacac
     4081 gttggtgccc accaccgaag cgtcgagggt caggttcttc cacttgtagg aagcgccgac
     4141 ggtgtagtcg aggtattcct tggtgtagtt gaagccgccg ccggtgtggc cgaggtgggt
     4201 gtggatcgtc agcgcgggca gcttggccgg gctgaagctg atctcgccat actcatacat
     4261 gttgtagtgg ctgatgccct gataggcatc gaaatagccc tggtccggcg cccagttcag
     4321 gccgcccttc acggtgaagg ggccataggc cttgctcagc gagccgtaga gctcgaaata
     4381 gttgccggcc ggcgcgcccg gatagaggta gccgtagagg ccgccgtcga aggtgatgcc
     4441 ggtcttgccg atggcgtggg tgtagccgcc atagacgtcg atctcggtgc cgccgatgtt
     4501 cacctggctc tggccggcgc tggcgctcga accccagaac gagacataga agccagactt
     4561 gtcggcaacg gtgatcgcgg cctgcacggc gggcttgttg tccgactgcg aaataccgcg
     4621 gaagcgatac tgcgagacaa cagccgcgct gccggtcacg gtgatttccg gagtcggcgc
     4681 gtcttcagcg aaagccacct ggctcgagca tgcgaatgcc acggcagcgg cggcgcagac
     4741 gaatttgatc ttcataagga gcccttttcc ctcttgatgt cgggttcctt cctgcatcct
     4801 ctactgtgca cctctttatc gggtgcagct tgtttaggcg aacaagggct atcagtgctt
     4861 attgcgccgc acaaccacca gcacggccat gcgcgacata tcggccgcat gtgtgccata
     4921 tcggcaacgc catgttgccg tcagatcaaa ccgcccaatc cttccacacc accgcctgcg
     4981 ccacttttac gcaggcattt tcagcatcat acgtcatcgc cggcgaacca tacaggcgcc
     5041 agccctgggc cagcgcctcg gaaacgcgct cgcaaaaggc gcgatcgtcc ttgccggtca
     5101 acaggcgata caccgggcgt tcatctggcg gcatcgtcac agcactttcc cctattggcc
     5161 agcgccggcg ccgcgggttt tccgcaaggc gccggcgccc tcattcaaca cactggcatc
     5221 aaaggatata tttggacagg tcagcatcgc tcgccagccc gtccaggcgc tggcgtacat
     5281 aggcggcatc gatcgtcacc tgcgcgccgc gccgctcctc ggcctcgaac gacagctctt
     5341 ccagcacctt ttccatcacc gtctgcaagc ggcgggcacc gatgttctcc accgtctcgt
     5401 tcacttgcgc ggcgatgcgg gcaatctccg ccaccgcatc cggagtgatc tctaccgcca
     5461 cttcctcggt ggccaacagc gcgcgatact gctccaccag attggcccgc gtgtccgaca
     5521 ggatccgcac gaaatccgcc tcgctcagcg ccgtcagttc cacgcggatg ggcaggcgcc
     5581 cctgcagctc tggcagcatg tcgctgggct tggccacatg gaaggcaccg ctggcgatga
     5641 acagcacgtg gtcggtcttc atcgggccat atttggtggc aatggtggtg ccctcgatca
     5701 gcggcagcag atcgcgctgc acgccctcgc ggctcaccga tccgccgcgc acatcggaaa
     5761 cggcgatctt gtcgatctcg tcgatgaaga cgatgccatt ggcctcggca tcggccagcg
     5821 ccacgcgggc gatgtcgtcc tggtccatgc gcttctcggc ctcctcgtcg atcagcttgt
     5881 cgagcgcatc ggccaccttc atcttgcggc gcttcatctg gtggcgaccc atggccttgg
     5941 ccatcatgtc gcccaggttg atcatgccca tgccgccgcc catgccgggg atctccatcg
     6001 gcgccgaagg ggtatcctcc acctcgatct ccacttccac atcggccatg gcgccctcgg
     6061 ccaggcgctg gcggaagctt tcacgcgtcg cctcgctggc gctatcgccc accagcgcct
     6121 tcagcagccg ctcctgcgcg gccctggcgg ccgcctcgcg cacggcctcc cggcggcggg
     6181 ccttttccag ccggatcgct tcctcggcca gatcgcggcc gatctgctcc acatcgcggc
     6241 ccacatagcc cacctcggtg aacttggtcg cctccacctt cacgaagggc gcatcggcaa
     6301 gccgggcaag ccggcggctg atctcggtct tgccgcagcc cgtcggcccg atcatcagga
     6361 tgttcttggg gctcacttcc tcgcgcaggg cagcgggcag gtgctggcgg cgccagcggt
     6421 tgcgcagcgc caccgccacc gccttcttgg cctcggcctg gccaacgata tgcgcatcca
     6481 gcgcggcgac gatcgccttg ggggtcagat tgtcgttcat gggaaagctc aaccgatcgt
     6541 ctcgaccgtc acgcggtcat tggtgaaaac gcagagttcg gccgccaccg ccatggcgcg
     6601 gcgggcgatc ttctcggggt cggtctcgta atcggccagc gcgcgggccg cggccagcgc
     6661 ataattgccg ccagagccga tcgcggcgat catcgtgtcg ccctgggctt caggttccag
     6721 cacatcgcca ttgccggtca gcaccagcag gctttcggca tcggccacga tcatcagcgc
     6781 ttccaggttg cgcagatatt tgtcggtccg ccagtccttc gccagttcca ccgccgcgcg
     6841 cagcagctga ccccggtggg cctccagctt gcgctccagc cgctcgaaca gggtgaaggc
     6901 atcggcggtg gcgccggcaa agccgccgat caccttgccc tcggcgccca gccggcgcac
     6961 cttgcgcgca ttgggcttca tcaccgtgtt gcccatcgac acctgcccat cgccggcgat
     7021 cacggttttg ccgccgcgcc tcacgccgat gatggtggtg ccatgccact ggatcagccc
     7081 gtggccggct ttcgtgcttt ccatgcggcc gatatgggag gggcggccag tcttttccag
     7141 accgccgccc cattcctttt cacgctatcg gccagagccg cctcgcaaag ggcgccctcg
     7201 catagggccc cgccgccaaa ggctcagctg ccgttggggc cgccaccacc accgccgccg
     7261 ccgccaccgc caggcccgct gccgccgctg ccaaggccgg ccgcgcccac cgtgccgatg
     7321 ttgccgaaca gatcttccag gaaggtgcgc tggcggccca gagtcggcgt ctcggacttt
     7381 tcaggcttca gggtcaccac cttgtcgagc ccggcgcgat ccacccgcgc cacattgccc
     7441 ttgtcatcga aggtcagccg cagcacggtc tccttcaccg gatggggccg gcgggccacc
     7501 ggctgggcgg tatccaccga gacgtaatac cacaccggcg tgccgaactg gctggtgaag
     7561 gtgggccggc ccagcgtgtg ctccaccgac aggcggttat ccacccccgg ctgcaccgaa
     7621 tcgagcagcg cctggtcgat cacatagccg cgatgatcgt ggatggtggc acacccgccc
     7681 gctgccagcg ccatgccggc cagcgccgcc agcttcatgc gcatcgaaaa cctgacctgc
     7741 atcgcgcgtc ttgctccaca ttccttgccg cgcctgctgc agggcttgcc cgcagcttcg
     7801 gctttgccta tcgcctctcg gccttagggc gccttgggcc atatcgcaat ggcgcttggc
     7861 aggcgcccgc ccgaattgcc tgttgcgaaa cgcccgccag aaaggaaaga cggccccgcc
     7921 gcgcccatga accacaccgc cactgccacc gcgccggccc ctgccggctt cctgtcccgc
     7981 ctgctgggcg gcctgttctc ccgccgggca gacccgcgcg aggcgttgcg cccgctgtgg
     8041 catggcgtgg tggccagcgc ccgccggccg gattggtaca ccagctgcgg catcgccgat
     8101 tcgctgcccg gccgcttcga tgcgatcacg ctggtgctgg cgctggtgct gctgcggctg
     8161 gagacagaag cggggcagac cgaggccggc gtgcgcctca ccgaactctt cgtcgaggat
     8221 atggatggcc agctgcgcga ggcgggcgtg ggcgatctgg tggtgggcaa gcatatcggc
     8281 cggctgatga gcgtgctggg cggccgcctg ggcgcctatc gcgcgggcct ggccgaggca
     8341 gacgatgccg aactgcacgc cgccctcacc cgcaatgtca cgttgacaga aggctccgcc
     8401 ccgcaggcgc tggccgctgc cacccgccag ctggccacag cccttgccgc cctccccgca
     8461 gagcgcctgc tggccggcga gatgccggca tgagcgcgcc cgaagccacc ggcgaattca
     8521 gccgcatcgt cgatctgcgc cagttcgacc aagcccccac ccacatcgcc gccagcacgg
     8581 ccgaatgcgc cgcccttgcc cgccgcttcg gcctggtgcg ggtggagcag ctggaagccg
     8641 atgtcgcctg cacggcagaa ggcgccagcg tgcgcgtcac cggccgggtg cgcgcccata
     8701 tcgtgcaaag ctgcgcggtt tcggccgagg acctgcccgt gacgatcgac gagcccatcg
     8761 ccttccgctt cgtgccgagc ggaaccccgc tgctggccac ggatgaggaa ggcatagaga
     8821 tcaccgccga ggattgcgac gagatcgcgt tcgatcaggg ctgcttcgat ctgggcgagg
     8881 ccgtggcgca aagcatggcc ctggcgatag accccttcgc caccgggccc gaggccgatc
     8941 aggtgcgcgc ctctggcctg ctgggcgcgg aaaacaccag cccctttgcc gcgctggctg
     9001 cgttgacaaa gaaggaagac taaaaaagag ggggcctttc gccccctcgt ccgatcttgc
     9061 cggtcagaac ggaatatcgt cgtcgagatc ctcggcgaaa ccgccgcccg aaccacccgt
     9121 gcggccccag tcgctgccgc ccgaggaacc accgcccgac gcgccgcccg gggaaccgcc
     9181 gctgcgcccc cagtcgcccc cgccggacga gctgccgcca ccgcgctgcc cgccgccata
     9241 gccgccgccc gaggaaccgc cgaaatcgcc gccccggccg ccgccaccct ggccgccacc
     9301 gggcgcgcca tccagcatgg tcagcacgcc gcccacgccg cccacgctga tttccgtggt
     9361 atagcggtca ttgccgttct ggtcctgcca cttgcgggtg cgcagctggc cctcgatata
     9421 caccttgctg cccttgcgca gaaaacgctc ggccacgccc accagcccgt cagactgcaa
     9481 caccaccgaa tgccattcgg tgcgctcctt gcgctcgccg gtgctgcggt ccttccagct
     9541 ttcgctggtg gcgatgcgca ggttggcgat gcgcccgccg ctctgaaacg acttcacttc
     9601 cggatcggcc cccagattgc cgaccagaat gaccttgttg acgctgcctg ccatctaacc
     9661 ctcttgcctc aaagtcccag cgccaccgcg ctccaatagg tgataccggc gaagaggtag
     9721 gccaagccga acagataggc cagcatgaag gccggccatt tccacccatt tgtttcacgc
     9781 cgtgtcacag cgatggtgct catgcactgg ggcgcgaaga cgaaccaggc cagaaaggcc
     9841 agcgccgttg ccaggctcca gtgcttctgc agctggccgg ccagcgcctt gccggtcact
     9901 tcctcgtcgc tgctggccac ggcataggta gtggcaagag agctgaccgc cacctcgcgc
     9961 gcggccatcg aggggatcag cgacagcgcg atatcgcggt tgaagccgat gggcttcacc
    10021 accaccgcca gcccgttggc gatatggccg gccagcgagg catcgatctg gtccttgccg
    10081 ggctcggcgc gggggaagtt gagcagcagc cacagcacca ccgacacctg gaagatgatc
    10141 gtgcccgccc ggcgcaggaa gatccacgcc cgctgcatca gccccagcgc cagatccttg
    10201 gcgcgcggca gctggtattt gggcagctcc atgatgaaac cgctggccgc ccccttggta
    10261 agggtggcgc gcagcagcag cgccaccacc atcgccccca cgatccccgc cacatacagc
    10321 gcgaacagca ccagcccctg caggccgatc agccccgcca gcgtgcccac agcgcccagc
    10381 ggccggttgg ggatgaacgc cgcaatgatc accgcataga ccggcagccg ggccgaacag
    10441 gtcatcaggg gggcgatcag gatggtcgtc agccggtcgc gcgggtcggt gatcgagcgg
    10501 gtggccatga tccctggcac cgcgcaggca aagctggata gcagcgggat gaagctgcgc
    10561 cccgaaaggc caaccccggc catcatccgg tccatcaaaa aggccgcgcg cgccatatag
    10621 ccagacgctt caaggctgag gatgaagaag aacaggatga tgatctgcgg cagaaacacc
    10681 accaccgcgc caacgccggc cagcacccca tcgatcaaca gatcgcgcca caggctggcg
    10741 ggcagttcgg cccgcaccag cgcgccaatc gcgtccacgc cggcctgcag ggcatcctgc
    10801 agcggcttgg cgcccgaaaa caccgcctga aacacgatga acagcgtggc aaacaggatg
    10861 atcggcccga tccacggatg cagcagcagc cggtccatcc gcgcatgcag gcggtggcgg
    10921 cgggtttccg acaggatcgc cgcatcagcg atcgcatggg cttccagccg ccgctcggcc
    10981 atgtcgagat gggcgcgggt gccggcgggc cgcgcccggg caatcgcctc gcccagctcg
    11041 ccaaggccgc gccggcgcac ggcaaccgtc gggatcaccg gcacgcccag cgccccttcc
    11101 agcgcgcccg gatcaatcac cagcccgtca cgctcggcca gatcgaccat gttgagcgcc
    11161 accaccaccg gcttgcccag cgcgatcact tcctgcgcga acaccagatg ctgctccagg
    11221 ttgctggcat ccagcaccac caccagcaca tcgggcgccg cctcgccggg cagctcgccc
    11281 aggatcaccc ggcgcgtcac ttcctcgtcg gggctggtgg catccagccc ataggagccg
    11341 ggcaggtcgg tcagctcgat cggctcgccg ctgggcagca ccatgcggcc gcccttgcgc
    11401 tccaccgtca cgcccggata attggcgatc ttctggcggg cgccggtcag cgcgttgaac
    11461 agcgcgcttt tgcccgcatt ggggttgccc accagcgcgg cggaacggcg gcggctcatg
    11521 cgccctgctc ctcatgccgg ccatgatggg gcccgccatg gccatgatgg cgcccgcgat
    11581 ggtgatgggg cccgcgcgcc ggcagcggtt ccacctgcat ggcgcgggca tgcacccggc
    11641 gcagcgccac gatcatccgc cccacgctga tcgccagcgg atcggcggcg aagaacacgc
    11701 ctttatgcgc cacccttatc ctggccccca catccagccc cagcgcgcgc aggcgcttgg
    11761 cttcctcggg caccagcgcg gcccaatcca ccgccacaat gcgcgccggg cagcgcagcg
    11821 gcaggcgatc aagagccacg gttttcacgg ccacagggtt ctgggcaaag ggcgcatcgg
    11881 caggcatcat cgcgcatcgc tgccagattg ccgcaggaat tgcaactggc tcgcaataag
    11941 ctggggcaat ctgtccagtt tcggcctgct atccgggcct taccgggccg gatagcgcag
    12001 ccggcccgcc agacgcacca ggctgaaagg cacccgctcg ggcggggtga agcgggcctt
    12061 ggccttttcg aagcgcagga tgccggcgat tcgcctgtcg aggaaggcca gcgtgtcggc
    12121 cttgccctcg ctcttgtcgt cgacaaacac cgcgagcgtc gccgcataaa ccgccgccag
    12181 cgtcgcccgc ttggtatagt ggttatagtc cgccgcggta tcgcctgcct ggcgccacat
    12241 ggcatcggcg ctgacccagc ccagcttcag cgcggcggcg atgttctgcg gcagggccat
    12301 gatcgccagc gcccggcgca gcgcttcctc gcggccctgc tgctcggcca ggcgcgcgcg
    12361 gatcagcccg gcaatgcgcc cgcgcacaga cagcgccgcc atcgcctcca ccgggcaggc
    12421 cgccgccatc gccgcatcga catgggcggt gaaggccgcg atcatcgcca tcgccccgcc
    12481 ctggaaggca taggccgcca catccgggtt cacccccagc ctggccgcgg ccgcgcgcac
    12541 ggcctccatg ctccagccat caaaggcggc ggcatcggcc agcgccggcg ccagcgcaat
    12601 gcgcagatcc tccagcgacc gctcttccag cggcggcagg gattcaggcg acagggtctg
    12661 gggcgcgctc acaggtgcgg cccatagccg gcggtgctgc gcaccttggc ctgcttggaa
    12721 tagctctcga tctcctcgag cagattgccg ctgcggtcgt tctcgcgcgc atattcgcgg
    12781 gcagagcggc cggtgttgtc ggcccggtcg gggttggcgc cggccttcag cagcacgcgc
    12841 atcatggcga tgtcgcggcg ctgcacggca gccatcagcg gcgtgtcgcc cagcgaatcc
    12901 gtctcgtcca cccgcgcgcc gttctggagg aggatatcca ccccctcggc aaaaccgatg
    12961 ttcgaggcca gttgcagcgg ggtcacgccg cggttgtcat gggtgttcac ttcagcaccc
    13021 ttgccgatga gaaaacgcat ccaggtggag tcgcgccgcc ccgtcacgat gtgcagcgcc
    13081 gtctcgccgc tggtgctgtc gtgggtgttg atcagcgtcg tgccgggctg cgacagatca
    13141 tcgttcacct cgtcgccatc cttcttgcgc acggcatcga ggaatttgta ggccttggaa
    13201 aactgcgcct cggccggggc caccagccct gccgccaaca ccagcgccag ccctgcgcga
    13261 accggcactc cacggaaaaa ccgcgcaaat ttcaacacct cgaacctcct ctggcgaagc
    13321 agcgcccccc agcaccacag agcaacgcaa tcgcaccttg cggggggcgc tttagcagag
    13381 catgaaccgc agcgccatga ccaagcctag ccccttcccc cttgcgcggc ccgcccgccg
    13441 cgcgctggcc gccctttcgc tggccggcct tgccctgctg ggcgcctgcc acgccaaacc
    13501 cgccgccgat gccgccggcg atgcgccgct ggccggcgct gccatcggcg gcgatttcac
    13561 cctggtggac aaggccggcc acaccgtgcg ctgggccgat tttgccggcc actaccgcat
    13621 cgtctatttc ggctacacct tctgccccga tgcctgcccc accgatctca acgtggcgat
    13681 gcgcgggctc gaccgtttcg ccaaggcgca cccgcaggcg gccgacagga tccgcccgat
    13741 cttcatcacc atcgaccccg cgcgcgacac gccggccgcc gtcggccagt ttgccgccgc
    13801 tttcagcccg cgcctgctgg gcctcaccgg cacgcccgat caggtggccg ccgccgccaa
    13861 ggccttcgcc gtctattatg cccgcggcaa ggatacgccc ggcggctatc tgatggacca
    13921 ttcgcgcatc gcctatctct tcggccccaa gggcgaggcg atcgacatgc tgccggtgga
    13981 caagggcccc gatgcggtgg cgcaggatct ggcgcggtgg gtgaaatgag cggccccgtg
    14041 cccttctggg aaaggcccgt ccacaccctc tcgcgcgagg aatgggaggc gctgtgcgat
    14101 ggctgcggcc agtgctgcct gcacaaggtg gaagatgccg ataccggcga tatctacccc
    14161 accaatgttg cctgcaagct gctggatatc ccgtcgggcc gctgctccga ttatgccggg
    14221 cggcgccggc aggtgcccga ttgcgtgcgc ctcacgccgc gcctcgccgc caccctgcct
    14281 tggctgccag acagctgcgc ctatcgcctg cgcgccgccg gagagcccct gcccgcctgg
    14341 cactatctcg tctgcggcga ccgcgaggcg gtgcatgccg ccggcgcctc tgtgcgcggc
    14401 aaggcgattt ccgaagtgct ggcagggccg ctcgaacagc atatcgtctg gccgggcgag
    14461 ccgatggaag tggtggccgt ggaagacgcg ccgtcgctgg acgactgagc gccatgcgct
    14521 ggccgcgccc ccgccccccc gcggcacagg cgccgcatct ggaactggcc ggccagcgcc
    14581 tgccggtgga aatccgcaag ctggcgcagg cccggcggat caccctgcgc ctggcccgcg
    14641 atggcagcgc ggtgcgcatc tccatgccgc gctgggcggc agaggcggag gcgctgcgct
    14701 ttgccctcag ccggcgcgaa tggctggccg cccagctggc ccgcctgccc cggccggcag
    14761 acccgcaggc gccgctcacc cacattgcct ggcggggcga gcggctggcc ctgcagcacg
    14821 atccggccgc cccccgccgc atccgcgcgg cgccccctgc gctccacatc ggcggcccgg
    14881 ccgacagcct gaagccgcgc ctgcaacgct ggctgaaggc cgaggcgctg cgcctgctgg
    14941 cggcagacct ggccgaatat tgcgcccgcg ccggcgtggc gcagccggcc ctggcgctat
    15001 cctcggcgca ggcgcgctgg ggcagctgct ccacccgcgg cgtggtgcga atcaactggc
    15061 ggctggtgat ggcgcccgat ggcgtgcgcc gctcggtcgt ggcgcatgaa gtggcccatc
    15121 ttgtccattt cgaccattcc cccgccttcc atgccctgct cgccagcctg ttcggcgccg
    15181 atctgcccgc ttgcgacaga tggctcaaag cccatggccg cacgctttat ctgcccttcg
    15241 gctaggctat ctgcccctcc agccaggcca tgcacaggca gcgctggcgc tttggcgcaa
    15301 ttgcgcctat tggggcggca tgctgttcaa gcgcaagggc cagttctggg atgatgtcag
    15361 cctgtcgggc ttcatcggtg attttctcac cgtgttccgt caggccgggc ggctgcgctg
    15421 gcggatctcg gcgctggcgg tggcctgcac ctatgcggtc ttctcgctga tcatccacga
    15481 cgagcaccgg atcgagccgc gcctgcccac catcacctat atcaccagct gggaagacgg
    15541 ccgcaccgat gccgaaatcc ttgccggcaa ccgcgccaac cagcgccgca aggaagccga
    15601 agccgcccgc gaagcggctg ccgatgccga tgtgaagggc atgtacaagg ccgtgggccg
    15661 cgcctcgggc ctcgatgtgg accagatgga gcggcaggcc aaggccgatg ccgccaagga
    15721 tgccgccgcg gccaaggcca agcaggccgc cctctatggc gccggccaca ccgccacacc
    15781 ctgaggtggc atccagcccg ccttctgcca cagatgcccg ctggctggcg gccaccgcgc
    15841 gcctggccat gcgcgcgcgg ccgctcagcc gccccaaccc cggggttgcc gcgctgatcg
    15901 tgaaagacgg ccgggtgatc gcccgcggat ggacagcgcc gggcgggcgc ccccatgccg
    15961 aagccgtggc gctggccgcc gccggcgcgc gggccgcagg cgccacgctc tatgtcacgc
    16021 tggagccttg tgcccatgac agcgcgcgcg ggcccgcctg cgccgatctg gtggcggcca
    16081 gcccgctcgc ccgcgtggtg gcaggggtga tggacccgga cccgcgcacc gccggcgcgg
    16141 gcctggcccg catccgcgcc gccggcaagc aggcgctgca cctgccctgc ccggccgccc
    16201 gcgccagcct tgaaggctat ctgatgcagc gcctcgccgg ccggcctttc gtcacgctga
    16261 agctggccac ctcgctcgat ggctgcatcg ccacggctgc gggtgaaagc cagtggatca
    16321 ccggcgctgc cgcccgcgcc cacacccatg ccctgcgcgc cgcggccgat gccattctgg
    16381 tgggcggcgg cacctggcgc gccgatgcgc cccggctgga tgtgcgcctg gccgggctgg
    16441 aaagccgcag ccccgcccgc tgggtgctca cccatgggcc ggtgggcgcc ggctggcagg
    16501 ccctggccgc gccagaagcc atccacaccg ccatgcccga tgtgcaatat ctgttcgtgg
    16561 aagggggagc gggcgctgcc gccgctttcc tcggggcggg ccttgtcgat cgggtgctgc
    16621 tctatcgcgc gccgctgttc attggcggcg gcaagccctg ccttggcgat gtgggccttg
    16681 gcccccttgc cgatgcccat ggccagtggc gccacgccga tcatcgccag cttggcaaag
    16741 acacgctcga catctacgag agggccccat gttcaccggc ataatcaccg ccatcggcac
    16801 tatcgcttcc gtgcgcgcct cgggcgatct gcacgccacc atcaccagcc cctacccggc
    16861 cggcggcatt gccatcggcg cttccatcgc ctgctctggc gtgtgcctga cggtggtgga
    16921 aaagggcgag cgggcgggcg gcagctggtt tgcggtcgat atctcgggcg agacggtggc
    16981 gcgcagcgtg cccggccgct gggccgaggg cgcgcagctg aacctcgaac aggcgctgaa
    17041 gctgggcgag gaactgggcg gccatatcgt caccggccat gtcgatggcg tcggccaggt
    17101 ggtggcgatc gagccggtgg gcgaatcgct gaaattcacc atcgccgccc cggccgaact
    17161 ggccccctat atcgccccca agggctcgat caccgtcgat ggcgtgtcgc tgacggtgaa
    17221 tgacgtgacc gacgaggccg atggcaccgc ccgcttcacg ctcaacatca tcccccacac
    17281 cgccgaggtg acgaccatcg gccagctggt ggcaggcgct ggcgtcaatc tggagatcga
    17341 cgtgctggcg cgctatctga agcgcatgca ggcgctgaag ggctgaggcg gggaagggct
    17401 gaggcgggga agggctgagg cagggcgcca gggcgcccgt ttcagccctt tgcggccagc
    17461 ccccgtgcaa aagcggtgcg ctccagcgca tagatcacat ggcgcacggc ctggccgccc
    17521 atcatcgcca catcgctgcg ctcggtcagc cgggcgccga tgcgttcggc agcgatgcgg
    17581 ctgcgccggt tcgcctcgcc aatgcggaaa tccacgcggg cgacatagcg gaaggcatgg
    17641 gccagcatca gccgcttcat cgcggcattg gcgcccgtgc cccaggcgct gcgggcaagg
    17701 aaggtccagc cgatctcgac agcgccgccg ccggccgggt catacgcatc ccagcgggaa
    17761 gagccgatca ccgcgcccgt cgccgcatcc accgccacta tcgccccgcc actggccagc
    17821 gcatcggcga agaaggcgcg gaacacctcc tcgcgccagc ggtcatgcgc ggggtgctgt
    17881 tcccagatca gcggatcgct ggcaacggca tagagcgcgt cccagtcttc aggcgcaagg
    17941 gggcgcagcg ccagccggcc cccttccaga accggctggc gctccatgcc ggtgatcagg
    18001 ccgtcacatc cgccacggcg gcgatgaact tgtcgatgtt gcccatcgtc aggccggcga
    18061 cgttgatgcg gccggagccg gccatgtaga caccatgctt ctcgcgcatg gccagcacct
    18121 gctcggggct gagcggcacg atcgagaaca ggccgttctg cgtgccgaag ggcgcgaaat
    18181 caaccgagcc ctgaatgccg gccccgccca ggcgggcgcg cacttcgttc atgcgggcgc
    18241 gcatcacgtc cagctcgttc agccagctgg cggtcagctc ggcatcttcc aggatgaggc
    18301 gcaccaccgc gccgccatga tcgggcggca tcgaccagtt ggcccgggcc agcgcatggc
    18361 cgttcgacag gatcgcgccg atctggctgg cctctgccgc cagcacatag agcgcgccga
    18421 cacggtcgcg ataatagccg aagttcttgt cgcagctgta ggcgatcagc gcctcgggca
    18481 cggcggccag cacgcggcgc acgccatagg cgtcctcctc catgccctgg cccaggccct
    18541 gataggccag atccagaatc ggcaggatgc ccttggcctt gatgagggcg gcaatctcgt
    18601 cccactcggc cggggtataa tcgatgccgg tggggttgtg gcagcagcca tgcagcatga
    18661 tggcatcgcc cgcctcggcg ctggcaatgg ccgtgcgcag ggcgtccata tcggcaacgc
    18721 cggccttggt ggcatgggca aagcccttga tcgccacgcc cacatcggcg gcgatctgcg
    18781 cgtggttggg ccagctgggc gtgcccatcc agatgcgctg gatgccggcg cgcttggcca
    18841 gcgccacagc caggcgcaca gcgccggtgc cgccgggggc ctgcatgccc tcgatccggc
    18901 catccagcgt ggggttcgcc tggccgaaga tatagggcat cagcgcatgg acaaagccca
    18961 tgtcgccctc aggcccaaga taggccttgg aatcctggat ctccagcagc ttcttctcgg
    19021 ccgccttcac cgcggcaaac accggcgtgg cgccggtgtc ggtgcggtag acgccgacgc
    19081 cgaggtcaat cttgtcctcg cgcgggtctg cattgtggag cttgatcagc gccagaagcg
    19141 cgtcggcggg ctgttgctgg aggttcgtaa gcatggcccg cgcccttaga cccgcgcgcc
    19201 cgcgcgggca agggcgtttg gcgcaatcgt cccgctcaga aaggcaacca gttctgatgg
    19261 cgcgacatct tcatgtagcc gccattgatg cccagccgca gccccacgcc cagccgcacc
    19321 ggaatcagca ccacatcgcc gcgccgcaga taggaaacat ggaagccgcc gatgaaatag
    19381 gcctgcccct cgcccgcgcc atagcggtgg aagagatcgt cggtgttgta gaggttatag
    19441 accagcacga aggtcttggc ggcgctggcg cccacatcga agcccagcga gggccccgtc
    19501 cagtacaccg gcatctgccc ttccaccttg tggaacagcg tgcccgagcc atagcgcagg
    19561 cccaccgcca gcgcgccgcc cgcctcgcgg ccaacgatat agccattggg ctcgccctgc
    19621 ttcttcagaa tatccttgat gacatcggcc agcccatgcg cgcccttgcc gaacacgccg
    19681 gtggccgcgc cgatgagatc gtcctcgtga taggtgccgt tgcggttatt cggctcgccc
    19741 gcgccattat agccgttgta gcgcggggcg gcggtaggat cgccctgata ggcgccgctg
    19801 ccgccggcat agggcgcggc cgccgtgccg gaaggcgcgc cggtgtaggc cgggcgcggg
    19861 ctggccgtgg cggaagcctg gggcgtgggc atcagatcgc tgtccacccc gccgctttgc
    19921 gcgccatagg cctgctggcc gtaagactgc tggccatagg attgttgcgc agccgccgcc
    19981 gtggccagcg ccggccccac cgccaggccc agcgcccctg ccaccagcgc cgcgcgggcc
    20041 agcccccggt gtgccaagcc ccggcgtgca agcaggcctg tcaggcgccc ggcccggcca
    20101 ctggcggcgc catgaaggtt gctgtgcttg ctcatcatcc tgtctcctgc cgaaccgccg
    20161 gccttgcgcg cctgccctag catgctttgc agcgggggag aatcctcggg caaggcgggc
    20221 atgcgggggc cggcgacagg ccgagtcggt tttgcgacag gccgcatggc gcagggatga
    20281 acgcccctgc cagccccgcc cggccctgat gctgggccac gccaaaataa accgcacaac
    20341 ccctcttgcc gccctgccaa agcgcggtta tagcgcgccc tcgccgctgc gatccggaga
    20401 cgtgggtgag tggctgaaac caacggtttg ctaaaccgtc atactgagaa ttcggtatcg
    20461 agggttcgaa tccctccgtc tccgccagat cccgcctctg gctcttcatc gcaaactggc
    20521 agaaacaggg ctggccaggc cctgctgccc ggaaccctca atatccgcca gggacgaagg
    20581 gatcaccctt tgcacgaaac ccgtgcacca gcgcccgcgc cgcgttggac agcaaaagcg
    20641 catccacgcc caccgccgcg aaatgcgccc cggttgcgag gaaatgctgg ctgaccccgg
    20701 ggcttgccga aaggaagccc gcaaacttgc ccaaagcgcg cactctggcg atcgccgcat
    20761 cgatggaggc gatcacgtcc ggatggtcgg gcttgcccag cagccccatc gaggccgaca
    20821 gatccaccgc gccaaaaaac acgccgtcca ccccggcggt ggcggcaata tcttccagat
    20881 tgttcagccc cgccacgctt tccacctgca gaatgaggca gatgttctca ttggccgtgg
    20941 ccatgtaaga gggatcgttg ccccatcggg cagcgcgggc gatgcctgat cccaccccgc
    21001 gaaacccttc tggcggatag cgggtggagg caacgatgcg ccgggcctgt gcgccgcttt
    21061 ccaccatggg gatcaacagg ttgttggcgc caatgtcgag caactgcttg atcagcaccg
    21121 gatcatccgt tggcgggcgc accaccggcg cgacatcata ggccgcaatc gcctgcaatt
    21181 gcccgaggat gatgcgcaga tcgacaggcg aatgctcgcc atccagcaac aggaaatcaa
    21241 agcccgcccc ggcgcagacc tcggcgcaat attgatccgc cagcgccagc cagaggccga
    21301 acagcggctg gcccgcaacc cgcgacttga agctattgtg catgcccgcc ctttctaaag
    21361 cttcacgcag acattggtga gttcggtgta gaactccagc ccgtgcaccc cgccctcgcg
    21421 gccaatgccc gattgtttgg cgccgccaaa ggccgtgcgc aaatcgcgca ggaaccaggc
    21481 gttgacccag cagatgccca cctccatccg cgccgccacg cggtgcgcgc gggcgaggtt
    21541 ctcggtccac accgccgctg ccaggccata gggcgaggca ttggccaggc gtatcgcctc
    21601 ctcctcggta tcgaacgggc ggatgtggca acaggggccg aagatctcct gcgtcacaca
    21661 atcggcatca tcggcaagcc cggtccagat cgtcggctga acccaggcgc cgccggccag
    21721 ggcggccggc atgtcgggca cgccgccgcc ggtgatcacc tgcgcgccct gctccaccgc
    21781 ccgggcataa tacgacagca ccttggcctt gtgctctgcc gaaaccagcg gccccagcgt
    21841 ggtagtggca tcttccggcc gccccgggcg cagcccttcg gcgccggcct tcatgcgcgc
    21901 cacaaaggca tcgaacagcg gcctttccac atacaccctt tcggtgccca ggcagacctg
    21961 cccgcaattg gcgaagaccg aacgcatcgt gccttccacc gccttgtcga gatcggcatc
    22021 ggcaaagatg atcgccgggt tcttgccgcc cagctcaaag gagaccgggc ggatgccttg
    22081 cgcggcctgc tgcatgatcg ccgcccccgt gcgcgtctcg ccggtgaagg tgatgccgtt
    22141 gatgccggga tggcgcgtga gatattcccc cgccgaggcc gggcccaatc catgcaccac
    22201 attgtagacg ccccggggca cgcccgccgc ctgcatcacc tcgcccagca gcgtggccgt
    22261 cagcggggtg tcctccgacg gcttgaccac caccgtgttg cccagcgcca gcgccgggcc
    22321 cactttccac gtcatcagca acaggggcag gttccacggg ctgatcaccg cgatcacgcc
    22381 gcgcgggcgg cgcaggccat agttgagcgc cccctgccca tcgggcgtgg gggtggaaaa
    22441 acattcggtg gccaccgtct tggcaatgtc ggtaaacagg cggaaattgg ccgcgccgcg
    22501 tgggatgtca atgtgggagg caagggcaaa gggcttgccg gtgtcgaggc attcagcctc
    22561 gaggaaatcg ttgaagcgcg cggtaatgcc atcggccacg gcgcccagca ggtcggtgcg
    22621 ctcggcaacg ctcatgcggc cccagggccc tgccagcgcg gcctgcgcgg cggcaacggc
    22681 ggcggccacc tcggcctcgc ccccttcggg caccaggccg atgacgctgc catcgatcgg
    22741 gctgtgattg gtccagctct tgccagactg gccacaggta aaggcaccat cgatgaaatg
    22801 cgcggcatcg cgccgcgcgt tgttggtatc aggcataatc cgtccaatgt tccagcgcgt
    22861 aggtcaggca tgcgcgctct tcctgttccg aggcgcccga agcgccgatc ccgccaatca
    22921 ccgcctcgcc cagcaggatc ggcaagccgc cgccaaacag caccacgccc ggcctttgcg
    22981 acagcccctg atgcagggcc ggactatagc tgagggccgc ccccagatcg tcggtggaaa
    23041 ggccgaagat ggcgctggtg gcggccttgt ccttcgccac gtcgatcgag ggcggccagg
    23101 tgccatcatc gcgacgcagc gccaccagat gcccggccgg atcgcagatc gcgatggcca
    23161 gacgcaggcc catggcgcgc gatcgtgcca tcgcgcgcgc cagaatggcc tcggccgcat
    23221 caagcgtgat cgcggcatgg gtgaaagaca aggccatggg gttttccttc aggttacaac
    23281 attcatgaaa ttgtcgttca gctggcgctg ataatagaag atgcccttgc ccaggctctg
    23341 cttgtcccac acccgcaccg ggtgatcggg ataataggta tagccttcgg caaagacttc
    23401 ggtgcgatta ccggatggat cgaagaagta gatcgtgcgc ccgcgggtga tgccatggcg
    23461 cgtggggcca agatcgatcg agaggttata cttggtgatg atatcggcgg catggccaac
    23521 ggcattccag tcggccagtt cgaaggcggc gtgatgcatg cgcccgggca ccccgccatc
    23581 cacaaaggcc acatcatgcg ccttcatgcc gcaggtcagc cagatggcga tgggagcgcc
    23641 gctttcatcg tccacctttt cggccagata gaaatccagc acgcgggtga agaaatcgag
    23701 cgtttcgggc acgcgcacgc cattcaactg catgtgatcg aaacggcgcg cgcccatgcc
    23761 atggggctcc accggccaga tatcgggatt ggccagatca ggcttgggct cagactgttc
    23821 ggcatgggcg aaaagctgga tcgtgtggcc cgatggggcg acgaatgaaa tgcgccggcc
    23881 atagccgggc tgctcaccag ccgcgatatg cgcgacagcg atgccctcgg cccgcacccg
    23941 cgcctcgaag tgatcaagat ctgcctcttc catcaccttg aaggcgatga aatccatccc
    24001 cggcgtatcg gcctcgacca gcgtgaccga atggtgctcg aactcgtccg ctcctttaag
    24061 gaaagcccgc cctgcatcgg aaccaacctt gtaaagcccg agacgatcca cataatgcga
    24121 aagcgcggcc tccacatcca tcacgcggat ctgaacaaga ccggggcgca ggataccttt
    24181 catagccatg gcatattttc cctttactgg gccagctgtg accaggtggc ccgaatgaat
    24241 gacggctttc ccccaccagt ttgcggtgat cttgttgtta tgaaatcatt caacgggatt
    24301 gaacggcagc cggccggtca ggccgaccgg cgcacgaccg acagcagcgg gaagcggctt
    24361 gccccccgcg ccagccccca gacccccttg cgcgcagcca ccatcatcac gatggcaatg
    24421 gcccccagca cgatctggta aaccgtgccg aaatccgcca gcagctcgcg cagcaggatg
    24481 aacagcgccg cgcccagcaa gggcccctcg atcgtgccat agccgccgat caccaccatg
    24541 aagatcacga aagcggtcca gtcgaccacg gcgaattgcg gatcgggcgc cacgcgcagc
    24601 ttctgcatca ccaccaccgc gcccaccagg ctggtcatca cccccaccac gcagaacagc
    24661 gcgatcttca gcggcttcac ccgcacgccc accgcctcgg cggcgatgcc gctgtcgcgc
    24721 agggcccgca ggcccagccc ccagcgcgaa cgcagcagcg caaaggtgat ccccaccgag
    24781 cccgccgcca ccgcaaaggc caccgcatag accacccgcg atcgcaccgc gcgcgtctcg
    24841 cccagcgtgt gcagcacatc agagggcagc gaggcccccg aaccgccgcc cagcgagggg
    24901 aattgcagca ccgtcaaccg caggatctcg gcaatcgccc aggtgccgat ggcgaaatag
    24961 gggcccgaca ggcgcagcga gagcagcccc atcggcaccg ccgccaccag ccccaccagc
    25021 gccaccagcg gcaaggcggc cagcatcggc acccctgcca gcacggtgaa gccataaagc
    25081 gcaaagccgc caatgccgat gaaggcctgc tgacccaccg aaagcaggcc gccatagctg
    25141 gccagcaggt tccacaaggt ggccagcgcc gcatagacga ggaattcgcc gagaatgcgc
    25201 tcggcagaag catcgcccca cagcggcacg ctggccagcg ccgccactgc cgctgcggcc
    25261 gccacacggg ccatggccag atcgcggggg ggcctggccg ggaggttggt cgtctgtgtc
    25321 atggtcatgc cttgctgcgc gccagcacgc cttgcgggtg cagcacgatc accgccagaa
    25381 acaccaggtg cccggccagt tgctggaatt gcgggttgat ctgggcgccc agcgcctggg
    25441 acacgcccag caggatgccc cccatcagcg ccccatagat actgcccatg ccgccgatca
    25501 cgatcacctc aaaggcgaag atcagccggc tgggcccgat gaaaggatca aaactgccat
    25561 aaacgcccat gaaaatgccc gaaagcgccg ccagcccgaa agcgatcgag gtgaccagcg
    25621 catagacatg gttggtatcc agccccatca cccgggcgat ttcggcatca tcggcagaag
    25681 cgcgaaaggc ccggcccgcg gcggtgtgaa agaacaccca ctgcagcccc accagcgcca
    25741 gcaccgccac gccaaacatc gccagcggca gcacgcctac cgccacgccc ggcatcaccg
    25801 ccaggctgag atcggccagg ggcccggcgt ccagccgccg gctatctgcc gaaaacagcg
    25861 agagcagcac gttctgcaaa atgatcgaga ggccaaaggt catgatcagc gcgggcagcg
    25921 gatcatggcc cagcgcccgg ttgaccagca gcctttgcag ccaatagccc gccgccgcca
    25981 cgatcagcac caccggcacc gccaccacaa agggcgacac ccccagcccc acggccagca
    26041 cctgcgccag aaaggcggcc accacgatga tatcgccatg cgccatgttg atgatgcgca
    26101 tcaccccgaa aaccagcgcc agccccgatg cagccagcgc atagataccg cccagcaaaa
    26161 gcccctggac aagggcgttg acccagatca tgcgtgaacc ccgaaatagg cgtcgatgat
    26221 ggcggcctgg tcggcggttc tgctgtcgcc cgacagcacc accttgccgg cgcgcagaca
    26281 caggaactga tcggacaccg acagcgcgcg gttgatatcc tgctccacca gaatggccga
    26341 aacccccggc ccgacaatct ggcggaacgc gcgatagatc tcctcgatca ccaccggggc
    26401 cagccccagg ctcagctcat cgagcagcag gagatcggga ttggccatca gcgcccggcc
    26461 gatcgccacc atctgctgct ggccgcccga aagctggggg cccagatggt ggcgccgctg
    26521 caccagggcg ggaaacagcg catagaccgc ctccagcgtc catggcccgg gccgcttcac
    26581 ctgcgcgccg atgaggaggt tctcctccac gctcagcgag gcgaacaggc gccgcccctc
    26641 gggcaccacg gccacgcccg cgcgggtggt ctcggcaggg gtgcggctgg caaggcaggc
    26701 gccattgtga tggatcgaac gcgcgggcgg cctgagcacg ccggccagcg tcttcagcag
    26761 cgtggtcttg ccggcgccat tcgcgccgat cagcgccagc accgcgcccg gcgcgatgct
    26821 gaagctgaca tcgaagaggg cctgaaagcc gccataaaag ctgctcaggc cagaaacgga
    26881 aaggcgcggc tcagtcatgg gccatccctg tgtaaagcgc caccacggcg gggctggaga
    26941 tcaccgcctg gggcgggcca tcggccacca cgcgcccggt atcgagcacc accaggcgcg
    27001 agacgaccgc catcagcgcg tgcacgatat gctcgatcca cacgatggtg acgccgccgg
    27061 catggatgtc gcgcacgatg tcgatcagtt gcgcgcattc ggcatcggtc agcccgccgg
    27121 cgatctcgtc gagcagcagg aggcgcggat tggtggcaag ggcgcgcgcc agttccacgc
    27181 gcttgcggtc cagcagcgtg aggtcgccgg ccagcgtgtc ggcgcggtgg gccagatggg
    27241 tctgggccag caccgcatgg gcgcgctgcg cggccgcgcg gcccgccagg ccgccgccaa
    27301 agctggcggc ggtcagcagg ttttcaaaca ccgtcatccc cgcaaagggc tggggcacct
    27361 ggaacgaacg ggcaatgcca tggcgcgaac gccatgccgc cggccgccgg ctgacatcct
    27421 gcccgacaaa gcccacctgc cccgcatcgg gcatcagcgt gccggtgatc aggctgaaca
    27481 gcgtggactt gcccgcgcca ttggggccga tgatgcccac cgcctgccct tccgggatcg
    27541 ccagcgagac atcatcggtg accttgagcg cgccgaaact gcgcgagagg cccgagagtt
    27601 cgagcagcgc gctcatgaca gcagcgcaag gttggcgccc accggaatgg ccggggcgtc
    27661 cttgtcgaaa acgatcttct gctcgacgcc gaagcggccg ccggccagcc attgcccgcc
    27721 caccagcggc gtggtcgaga cattggccac cggccccctg gcccaatcca catggcccgc
    27781 gatcgtatcg aggctggtgg tcttgatcgc gtcgcggatc gcttcgggtt ccagcgccgt
    27841 ggtgcgccgc agcacatcaa ccgccacctc gaacagggca tgtttgaagc ccagcggcgg
    27901 g
//
LOCUS       DASRYE010000016         4924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_213084,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000016 DASRYE010000000
VERSION     DASRYE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213084"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFF98_00865"
     CDS             <1..617
                     /locus_tag="VFF98_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU62204.1"
                     /translation="LVPADVLIASSTSSLPVSTLQAGLAHGGRCVLGHPFNPVHLIPL
                     VEVGGGAASDPAAVDIAQDFYASLGKQPIRLRREIFGHIGNRLTSAMFREAVRLVAEG
                     YASVADIDKAIRHGPALKWAIQGQFTTFHTSGGAGGLAAFLPKFAPGIVERWATMADP
                     PLTDPALQAQLVRGMEEAAQGRSVEAIAAHQDACLMALLQLLRD"
     gene            727..1713
                     /locus_tag="VFF98_00870"
     CDS             727..1713
                     /locus_tag="VFF98_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZU62205.1"
                     /translation="MTVDAINRPDAEDMSVAFVKPVRELQSCNHLLDDHAALEAFYED
                     QGYILLRGVFDPQSVAEARDAMLAVAAALGLTTPGDPTGTWTGAAFAGGMEESDSYSG
                     IARKLIEHPANLAVMEKVLGEPAVAVPIVQYRTYPPHGPITVVHQDGFYSPGIQDYKP
                     VWVPLSPCDRSMGGLAVAVAQNKRGYFHNIGKPAPYPFPRDAVPDDAWATTDYLPGDV
                     LIVHPYTPHCSLANTSSRLRITFDSRVQSARNPSAIAATVLAVSPTSITVDAGGPAPQ
                     TFSVDKDTFLRPVNPGVREPFESFAEVTRPGMRLVVVRDGNRAVMLRKAAEG"
     gene            complement(1810..2937)
                     /locus_tag="VFF98_00875"
     CDS             complement(1810..2937)
                     /locus_tag="VFF98_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62206.1"
                     /translation="MDFELTDEQKLLGEMVNRFVADNCPFQKREQAQKSALGWSRENW
                     AGLAEMGLLALPFAEADGGFGGGGVEQMIVMEALGRGLVAEPYFATVVLAGGVLRHGA
                     SPAQRARLVPELAAGTHLLALAHAERGLPRHTLEARTTATRSGDGWRIDGRKSAVLHG
                     QSADTLIVSAQMEGGLGLFLVPAGADGLQLAGARGYDGVPVAEIALAGVAVAGDALIG
                     PAGGGAAILESAFAEATAALVAEAVGVMAETLDITVEYLKTRKQFGVAIGSFQALQHR
                     AVEMLMQLELSRSMAILAALALRGDERERSRAIAAAKAQIGKAGRLIGQEAVQMHGAI
                     GITAEYKVGHAFKRLTAIDALLGDRDHHLARLQEMGGLYAA"
     gene            complement(2968..4155)
                     /locus_tag="VFF98_00880"
     CDS             complement(2968..4155)
                     /locus_tag="VFF98_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62207.1"
                     /translation="MHFDFTPDELAFREEVRAFLAAALPPDLRQKMIDREHLSKADIT
                     RWQRLLNARGWAVPHWPVAHGGQDWSPAQRYIFQEEMALAHAPEGSPFNVNMIGPVLC
                     RYGTPQQQARFLAATANLDIWWCQGFSEPGAGSDLASLKTTARREGDHYVLNGQKIWT
                     TQAQHADWMFGLFRTDSSGRKQQGITFLLLDMRTSGITVRPIETIDGEHDANEVFFDD
                     VRVPVENRVGEEGAGWDVARHLLGSERSGIARIGLSKERLLQLRALEQRLAADGSLTA
                     AEQHRLGQKLAEVEVELRALELTQLRVVAADTYGGGAAAAASILKVKGTELQQRMTEL
                     VLELAGPAGLAMPDHHRPAPDPLHDWIATAPPFYFAMRKVSIFGGSNEIQKNIIARTV
                     LGL"
     gene            4330..4761
                     /locus_tag="VFF98_00885"
     CDS             4330..4761
                     /locus_tag="VFF98_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZU62208.1"
                     /translation="MADLDAKEQSGIAFIGGIMGEAFGKAFSEAAEADRFGSAITRMA
                     ASWAFHDAWQHEGLARREKSIAILSALIAMRQPAELKNHVKIGIANGLGAAEIEGLLI
                     QLTPYVGFPCIASATTAVIEALREAGVSPEGAKTSEERGLL"
BASE COUNT          787 a         1770 c         1584 g          783 t
ORIGIN      
        1 ggctggtgcc cgccgatgtg ctgatcgcca gcagcacttc cagcctgccg gtcagcacgc
       61 tgcaggcagg gctggcccat ggcgggcgct gcgtgctggg ccatccgttc aacccggtgc
      121 atctgatccc gctggtggaa gtgggcggcg gcgcggccag cgatccggcg gcggtggata
      181 tcgcccagga tttctacgcc tccctgggca agcagccgat ccgcctcagg cgcgagatct
      241 tcggccatat cggcaaccgg ctgaccagcg cgatgttccg cgaggcggtg cggctggtgg
      301 ccgaaggcta tgccagcgtg gccgatatcg acaaggccat ccgccatggc ccggcgctga
      361 aatgggccat ccagggccag ttcaccacct tccacacatc gggcggggcg ggcgggctgg
      421 ccgccttcct gcccaaattc gcccccggca tcgtggagcg ctgggccacc atggccgatc
      481 cgccgctgac agatccggcg ctgcaggcac agctggtgcg cggcatggaa gaagctgcgc
      541 agggccgcag cgtggaggcc atcgccgccc atcaggatgc ctgcctgatg gcgctgctgc
      601 agctgctgcg cgattgatga ttttcatccg ctagtagatc agaaacatcc ctcaccccgc
      661 cgcctccctg ttgtcttatc ggcgcatcaa cggcgctgct ggcagcgcct tatgggagag
      721 acgaggatga ccgtggacgc catcaaccgg cccgatgccg aagacatgag cgtggccttt
      781 gtgaagccgg tgcgcgagct gcaatcctgc aaccacctgc tggacgacca cgccgcgctg
      841 gaggccttct acgaggatca gggctatatc ctgctgcgcg gtgtgttcga tccgcaatcg
      901 gtggcagaag cgcgcgatgc gatgctggcg gtggccgccg cgctggggct gaccacgccc
      961 ggcgacccca ccggcacctg gacgggcgcc gcctttgccg gcggcatgga ggaaagcgac
     1021 agctacagcg gcattgccag aaagctgatc gagcatccgg ccaatctggc ggtgatggaa
     1081 aaggtgctgg gcgagccggc ggtggcggtg ccgatcgtgc aatatcgcac ctatccgccg
     1141 catgggccga tcaccgttgt ccaccaggac ggcttctaca gccccggcat ccaggactac
     1201 aaaccggtgt gggtgccgct cagcccgtgc gatcgctcga tgggcgggct cgcggtggcc
     1261 gtggcgcaga acaagcgcgg ctatttccac aatatcggca agcccgcgcc ctatcccttc
     1321 ccgcgcgatg ccgtgcccga cgatgcctgg gccaccaccg actatctgcc cggcgatgtg
     1381 ctgatcgtgc acccctacac gccccattgc agcctggcca acacctccag ccggctgcgc
     1441 atcactttcg acagccgcgt gcaatcggcc cgcaacccca gcgccattgc cgccaccgtg
     1501 ctggcggtca gccccacctc gatcacggtg gatgccggcg ggccggcgcc gcagaccttc
     1561 tcggtggaca aggacacctt cctgcgcccg gtgaaccccg gcgtgcgcga acccttcgag
     1621 agctttgccg aagtgaccag gccgggcatg cggctggtgg tggtgcgcga tggcaaccgc
     1681 gcggtgatgc tgcgcaaggc cgccgagggc tgagcgaaaa ggggggcgtt tgccgcgccc
     1741 cccttccccc acggcccctc ttcccggacc ggaactgata gcgcccgcac aggcggcgcc
     1801 gacagcggct caggcggcgt aaagcccacc catctcctgc aggcgcgcca gatggtgatc
     1861 gcgatcgccc agcagcgcgt cgatcgccgt cagccgcttg aaggcatggc ccaccttgta
     1921 ttcggcggtg atgccgatgg cgccatgcat ctgcaccgct tcctgcccga tcagccgccc
     1981 ggccttgccg atctgtgcct tggccgcggc aatggcgcgg ctgcgctcgc gctcgtcgcc
     2041 cctcaacgcc agggcggcca ggatcgccat gctgcgcgac agttccagct gcatcagcat
     2101 ctccaccgcg cggtgctgca gcgcctggaa gctgccgatg gccaccccga actgcttgcg
     2161 cgttttcaga tattccaccg tgatatccag cgtttcggcc atcacgccaa cggcttcggc
     2221 caccagcgcg gcggtggcct cggcaaaggc gctctcgaga atggccgcgc cgccgccggc
     2281 agggccgatc agcgcatcgc cggccaccgc cacgcccgcc aaagcgatct cggccaccgg
     2341 cacgccatca tagccgcgcg cgccggcaag ctggaggcca tcggcgcccg ccggcaccag
     2401 aaacaggccc aacccgccct ccatctgcgc gctgacgatc agcgtatcgg cgctctgccc
     2461 gtgcaggacc gcgctcttgc gcccgtcgat ccgccagcca tcgccgctgc gggtggcggt
     2521 ggtgcgggct tccagcgtgt ggcgcggcag gcccctttcg gcatgggcca gcgccagcag
     2581 atgcgttccg gcggccagtt cgggcacaag gcgcgcgcgc tgggcggggc tggcaccatg
     2641 gcgcagcacg ccgccggcca gcaccacggt ggcgaaatag ggctctgcca ccagcccgcg
     2701 ccccagcgct tccatcacga tcatctgttc cacgccgccg ccaccaaagc cgccatccgc
     2761 ctcggcaaag ggcagggcca gcaggcccat ttcggccagc cccgcccagt tctcgcggct
     2821 ccagcccagt gcgcttttct gggcctgctc gcgcttctgg aacgggcaat tgtcagccac
     2881 gaagcggttc accatctcgc cgagcagctt ctgctcgtcc gtcagttcga aatccatgct
     2941 atctgcttcc tgcttggccg gcccgcatca caggcccagc acagttctgg cgatgatgtt
     3001 cttctggatc tcgttcgagc cgccaaagat cgacaccttg cgcatggcga aatagaaagg
     3061 cggcgcggtg gcgatccagt cgtgcagcgg atcgggcgcg gggcggtggt gatcgggcat
     3121 ggcaaggccg gccgggcctg ccagttccag caccagttcg gtcatccgct gctgcagctc
     3181 ggtgcccttc accttgagga ttgaggcagc ggccgccgcc ccgccgccat aagtatcggc
     3241 ggccaccacg cgcagctggg tcagctccag cgcgcgcagc tccacttcca cctcggccag
     3301 cttctggccc agccggtgct gttcggcggc ggtcaggctg ccatcggcgg ccaggcgctg
     3361 ctccagcgcg cgcaattgca gcagccgctc cttcgacagg ccgatgcggg cgatgccgct
     3421 gcgctcgctg cccaacagat gccgggccac atcccagccc gcgccctcct cgcccacgcg
     3481 gttctccacc ggcacgcgca catcatcgaa gaacacctca ttggcatcgt gctcgccatc
     3541 gatcgtctcg atcgggcgca cggtgatgcc gcttgtgcgc atatccagca gcaggaaggt
     3601 gatgccctgc tgcttgcggc cggaactgtc ggtgcggaac aggccgaaca tccagtcggc
     3661 atgctgggcc tgggtggtcc agatcttctg gccgttcagc acatagtgat cgccctcgcg
     3721 ccgggcggtg gtcttcaggc tggcgaggtc ggatccggcg cccggctcgg aaaagccctg
     3781 gcaccaccag atgtcgagat tggcggtggc ggccaggaag cgcgcctgct gctgcggcgt
     3841 gccatagcgg cacagcaccg gcccgatcat gttgacgttg aagggcgagc cttccggcgc
     3901 atgggccagc gccatttcct cctggaagat atagcgctgc gccggcgacc aatcctgccc
     3961 gccatgggcc accggccaat gcggaaccgc ccagccgcgc gcgttgagca ggcgctgcca
     4021 gcgggtgata tcggccttgc tcagatgttc cctgtcgatc atcttctggc gcagatccgg
     4081 tggcagggcg gccgccagga aggcacgcac ctcctcgcgg aaggccagtt catcgggtgt
     4141 gaaatcgaaa tgcatcgcct gttccccatg cggatcaggc ccaacagccg ccgatgcgcg
     4201 gccctgtcaa cgcgccgccc ccaagccttc acatggccga tggattgccc gcgccggccc
     4261 ttgcggttga cgctgccccg cccccgcccc tacccccggc agacaacaac agggatgaag
     4321 gaacgcccca tggccgatct cgacgcaaag gaacaatccg gcatcgcctt tatcggcggc
     4381 atcatgggcg aggctttcgg caaggccttc agcgaggcgg ccgaggcgga tcgtttcggc
     4441 tctgccatca cccgcatggc cgccagctgg gccttccacg atgcctggca gcacgaaggc
     4501 ctggcccgcc gcgaaaagag catcgccatc ctctcggcgc tgatcgccat gcgccagccg
     4561 gccgagctga agaaccatgt gaagatcggc attgccaatg gccttggcgc ggccgagatc
     4621 gaggggctgc tgatccagct gacgccctat gtcggctttc cctgcatcgc ctctgccacc
     4681 acggcggtga tcgaggccct gcgcgaggcc ggcgtttcgc ccgagggcgc caagacctcg
     4741 gaagagcgcg ggctgctctg agcaccaggg gcggccccat gcacgacatg cacgatatgc
     4801 gcgatcttac ggacagcccg cacgcatccg ggctcgacat caccctctcc cacacgctgc
     4861 ccgaagtgat cgcccatgtc gcccggcgcc atggcgtgcg gcccttcatc atcgacgagg
     4921 cggg
//
LOCUS       DASRYE010000017         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_53619,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000017 DASRYE010000000
VERSION     DASRYE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53619"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..675
                     /locus_tag="VFF98_00890"
     CDS             136..675
                     /locus_tag="VFF98_00890"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62209.1"
                     /translation="MLLVPGHDDAPARRWLANWESRFPEAITVELGLWDRPHRNTWVN
                     RLNLAIHRAGRPVVIVAEGLATMAVAWWAEYEQPGAGGPVRGALLIDPPDVDRPGGDA
                     RLASFPSCPRAALPFAACVLASRSRGPLALRTIRQLAADWGGPFDTLASAAVLAAPSL
                     SPVQSLGQKVLSGWMSATA"
     gene            912..1562
                     /locus_tag="VFF98_00895"
     CDS             912..1562
                     /locus_tag="VFF98_00895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62210.1"
                     /translation="MNRHMSGAAQPGAAEGQEDVLVLTVPGLNNSGPEHWQSLWEQQR
                     GDCLRVELGQWDRPHRNSWVNNLNLAIRAAEGPVYLAAHSLGCLAVAWWAQMEPKAAQ
                     LVAGALLVAPPEVDFTPEDPRLVGFSPTPCAPLPFPSVLVASSNDPYMDAHAARYLAR
                     MWGAEFVNVGRMGHINACSQIGDWPQGQALLDGLIAQGQRLRARQTTTSQGWAARP"
     gene            1569..2273
                     /locus_tag="VFF98_00900"
     CDS             1569..2273
                     /locus_tag="VFF98_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZU62211.1"
                     /translation="MKRALIIRHVPHEGVAGYRAPIEAAGYDVDRIDVTDPAFAGVDL
                     CAPDLLIMMGGPMGVYEADIYPWINCQKRRLAQRLAADRPTLGVCFGAQMMAAALGAH
                     VYAGPRKEVGFHPVRVHDVPDSPLHHVADVPVLHWHGDTFTRPDNVEILASSHVYDHQ
                     AFRRGRNILALQFHAEMGLDPRFDAWIEQWPESVVEAGGTEADLRAAHATHGAGAVAA
                     GRAMIGQWLSGLALSA"
     gene            2330..>2794
                     /locus_tag="VFF98_00905"
     CDS             2330..>2794
                     /locus_tag="VFF98_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62212.1"
                     /translation="MHKLTRSQSQAQTRARLIEAAQQLFMRDGFAATSIDAIAEAAGY
                     SKGAVYSNFGSKEDVFLAALHEGGRANLAGLLEGLGHTRSADEVIEVMAGWADRQAGA
                     GNWTLTILEHARLVQDDAASLRQQERILREAWQRLGSWMVERFPQLGEDAELV"
BASE COUNT          427 a          879 c          993 g          495 t
ORIGIN      
        1 cgctttgttt gagcatagct tcctcttgtc gggacgtatg ccgagtcgac cgacttcacc
       61 caagagggag aggttgtcat ggcttcagaa catcctgtat tcgcgcccca gagtggcgca
      121 gcgcacagcc cgctcatgct gctggtgcct gggcatgatg atgcccccgc ccgccgctgg
      181 ctggccaatt gggaaagccg ctttcccgaa gcaatcaccg ttgaactggg cctgtgggac
      241 aggccgcacc gcaatacatg ggtcaatcgc ctcaatctgg cgattcatcg cgccgggcgg
      301 ccggtggtca tcgtggcgga agggctggcg acgatggcgg tggcctggtg ggctgaatat
      361 gagcagcccg gcgctggcgg gccagtgcgc ggcgccctgc tgatcgatcc gcccgatgtt
      421 gaccggccgg gcggcgatgc gcgcctggcc tctttccctt cgtgtccgcg agcggcgctg
      481 ccgtttgcgg cctgcgtgct ggcctcgcgc agcagggggc cgctggcgct gcgcacgatc
      541 cggcagttgg cggccgattg gggtgggcct ttcgatacgc tggcctcggc ggcggtgctg
      601 gctgcgcctt cgctcagccc ggtgcagtcg ttggggcaga aggtgctgtc gggctggatg
      661 agcgcgacgg cctagcgcct gcttcgccct gactgtgcgg gatctgcgcg gcaaaggcgg
      721 cgcgggccga tacctttgct tttaccgccg acaggccgga ttgcccgaaa tagcgcggct
      781 ttccggcctt gcaaaaactc tatcgaatga attgacttta gcgccttgcc cagggccatc
      841 gccctggcat aggcagaaag gcacgggggc gccgcgcccc ttcatccatc tcgagccggg
      901 ggagtattgc catgaaccga cacatgtctg gcgccgcgca gcctggcgcc gcagaaggac
      961 aggaagacgt gctggtgctg accgtgcccg gcctgaacaa ttccgggcca gagcactggc
     1021 aaagcctgtg ggagcagcag cgcggcgact gcctgcgcgt ggaactgggc cagtgggacc
     1081 gcccgcatcg caacagttgg gtcaacaatc tgaacctggc gatccgcgcg gccgaagggc
     1141 cggtctatct ggccgcgcac agccttggct gtctggcggt ggcgtggtgg gcgcaaatgg
     1201 agccaaaggc tgcgcagctt gtggccggcg ccctgctggt ggccccgccc gaggtggact
     1261 ttacccccga ggacccgcgc cttgtcggct tttcgcccac gccctgcgcg ccactgccct
     1321 ttccctcggt gctggtggcc agcagtaacg atccttatat ggatgcccat gccgcccgct
     1381 atctggcgcg catgtggggg gctgaatttg tgaacgtggg gcgcatgggg catatcaacg
     1441 cgtgcagtca gattggcgac tggccgcaag ggcaggccct gctcgacggg ctgattgcgc
     1501 aggggcagcg cctgcgcgcg cgccagacca ccaccagcca gggctgggcc gcccggccct
     1561 gagacagcat gaaacgcgcg cttatcattc gccatgtgcc gcacgagggg gttgccggtt
     1621 atcgcgcgcc catcgaggcg gcaggatatg atgtcgaccg tatcgacgtg accgatccgg
     1681 cctttgccgg cgttgacctg tgcgcgcccg atctgctcat catgatgggc gggccgatgg
     1741 gggtgtacga ggccgacatc tatccgtgga tcaactgcca gaagcgccgg ctggcccagc
     1801 gccttgcggc tgatcgcccg acgctgggcg tttgttttgg cgcgcagatg atggcagcgg
     1861 cccttggcgc gcatgtctat gccggcccgc gcaaggaggt ggggtttcac ccggtgcgcg
     1921 tgcacgatgt gcccgacagc ccgctgcacc atgtagctga cgtgccggtg ctgcactggc
     1981 atggcgatac cttcacccgc cccgacaatg tcgagattct ggcctcgagc catgtgtacg
     2041 atcatcaggc gttccggcgg gggcgcaaca tactggcgct gcagtttcac gccgaaatgg
     2101 ggctggaccc gcgtttcgac gcctggatcg agcaatggcc cgaaagcgtg gtcgaggccg
     2161 gcggcaccga ggcggacctg cgcgcggccc atgccaccca tggcgctggg gcggtggcgg
     2221 ccgggcgggc gatgattggc cagtggctgt cggggcttgc cttatcggct tgaaatcgct
     2281 tctggctttc gcgggcgact tggccgaaca tggccggccg gagaagcaga tgcacaaact
     2341 gacccgcagc cagagccagg cccagacccg cgcccgcctg atcgaggcgg cgcagcaatt
     2401 gttcatgcgc gatggttttg ccgccacctc gatcgacgcc attgccgagg cggcgggcta
     2461 ttccaagggg gcggtctatt ccaatttcgg cagcaaggag gatgtgttcc tggccgcgct
     2521 gcacgaaggc ggcagggcca atctggccgg cctgctcgag gggctgggcc acacacgcag
     2581 cgctgacgag gtgatcgagg tgatggccgg ttgggccgac cggcaggccg gcgccggcaa
     2641 ctggacgctg accatccttg aacatgcccg gctggtgcag gacgatgctg cctcgctgcg
     2701 ccagcaggag cggatcctgc gcgaggcgtg gcagcggctg gggagctgga tggtcgagcg
     2761 ctttccgcag ctgggcgagg atgccgaact ggtg
//
LOCUS       DASRYE010000018         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_88920,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000018 DASRYE010000000
VERSION     DASRYE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88920"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1065)
                     /locus_tag="VFF98_00910"
     CDS             complement(133..1065)
                     /locus_tag="VFF98_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62213.1"
                     /translation="MLQGSLPAGELGNDIKVAVATAERGVSRRMAGLFSVIVSLAMLV
                     AVAFEVRDLNFREILAMIPHTPGFWMAFAAYYFVPPLTEWAIYRRLWGIPLGGVGALL
                     RKQVSNELLLGYLGEVQFYAWARGKLNMVTAPFGAIKDVTILSALTGNIATLAMLAGA
                     WPLVASGALGMESRTVFMSLGVVLLTSFVILLFRQKLFTLPRRELLIITGMHFSRILA
                     IVGLWALMWHLVLPEVAFEMWLVLATLRMLISRLPLIPNKDVVFAGLAVFLLGHETQI
                     SHLIAMMAGLLPAAHVVVGTIFATADVVTSRSER"
     gene            complement(1247..2023)
                     /locus_tag="VFF98_00915"
     CDS             complement(1247..2023)
                     /locus_tag="VFF98_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU62214.1"
                     /translation="MSKLLPSKLLLASIHDVGPRSEAQVDQLIDIMAPILGGMNMAML
                     VVPDHWGEAPLAQAPAFQAKLRGWAEQGVEMFVHGWFHKDLAQHTGMAAIKARHMTAS
                     EGEFLGLSHAEAARRMADGRKLIEDIIGRPTAGFIAPAWLYGEGARAALAESGFPLAE
                     DHFRVWRPDSGQILTRGPVITWASRSKGRQRSSRFFAALARNALTGQGVARIATHPGD
                     VTVPALVDSIRATYTRFMQGRRAGRYAELLRPSGTVQAAF"
BASE COUNT          388 a          679 c          702 g          303 t
ORIGIN      
        1 ccatcagcgc ggggccggtt tcgcccgcgc ggcacttggc ctcggccttg cccagttcgg
       61 gcgtcgaggg cagggggacg accggcttgg cggcatcggc ggcaaacagg ggcaaaaggg
      121 ccagaagggg gctcatcgct cgctccttga agtgacgaca tcggcggtgg caaagatggt
      181 gccgaccacg acatgggcgg cgggcagcag cccggccatc atcgcgatca ggtgcgagat
      241 ctgggtttca tggcccagca ggaacacggc aagcccggca aagaccacgt ccttgttcgg
      301 aatgagcggc aggcgcgaga tgagcatgcg caaggtggcc agcaccagcc acatctcgaa
      361 ggccacctcg ggcaggacca ggtgccacat cagcgcccac aggcccacaa tcgccagaat
      421 gcggctgaaa tgcatgccgg tgatgatgag cagctcgcgc cggggcaggg tgaagagctt
      481 ttgccggaac agcaggatga cgaaagaggt gagcagcacc acccccagcg acatgaagac
      541 cgtgcgcgat tccatgccca gcgcgcccga ggccaccagc ggccaggcgc ccgccagcat
      601 ggccagcgtt gcgatattgc ccgtgagcgc ggacaggatg gtcacatcct tgatcgcgcc
      661 aaagggggcc gtcaccatgt tcagcttgcc gcgcgcccag gcatagaact gcacctcgcc
      721 cagatagccc agcagcagtt cgttcgacac ctgcttgcgc agcagcgcgc caacgccgcc
      781 cagcgggatg ccccacaggc gccggtaaat cgcccattcg gtgagcgggg gcacgaagta
      841 ataggccgca aaagccatcc agaagcccgg cgtgtgcggg atcatggcca gaatttcgcg
      901 gaagttgagg tcacgcacct cgaaggccac ggccaccagc atggccagcg agacaatcac
      961 gctgaacagc ccggccatac gccgcgacac accgcgttcg gcggtggcca cggcgacctt
     1021 gatatcgtta cccagctcgc ccgcaggcag gctgccctga agcattggtc cagatccttc
     1081 gcccgtgccg gtgccatccg gtttggggcg attgttgttt ccctggcccg acccgttgtt
     1141 ggcgggcggt tcgggaagcg ttgtgtcctc ggtcatggcg ccgccttggc agcgttcggc
     1201 gcgaaaggcc agtaggccct tttcacggcg gaattagggc acataatcag aacgcagcct
     1261 gaacggtgcc cgacgggcgg agcagttcgg cataacgccc ggcgcgccgg ccctgcatga
     1321 agcgggtgta agtggcgcga atcgagtcga caagcgcggg cacggtcacg tcgccgggat
     1381 gagtcgcgat gcgcgccacg ccctgtccgg tcagggcatt gcgggcaagg gcggcgaaaa
     1441 accgcgacga gcgctgccgc cccttgctgc gcgaggccca ggtgatgacc ggcccacggg
     1501 tcaggatctg gccgctgtcg gggcgccaca cgcgaaaatg gtcctcggcc agggggaagc
     1561 ccgattcggc cagcgccgcg cgggcgccct cgccatacag ccaggccggc gcgatgaagc
     1621 cggccgtggg ccgcccgatg atgtcctcga tcagcttgcg cccgtcggcc atgcggcgcg
     1681 cggcctcggc atggctcagg cccaggaact cgccctcgct ggcggtcatg tggcgggcct
     1741 tgatggcagc catcccggtg tgctgcgcca gatccttgtg gaaccagcca tgcacgaaca
     1801 tttccacgcc ctgttcggcc cagccgcgca atttggcctg aaaggccggc gcctgcgcca
     1861 aaggcgcctc gccccagtga tcgggaacca ccagcattgc catgttcatg ccgccaagaa
     1921 tgggggccat gatgtcgatc agctggtcga cctgcgcctc ggagcggggg ccaacatcgt
     1981 ggatcgaggc cagaagaagt ttggaaggca gaagcttgct cattgctctt cctccagcgc
     2041 ggtcagtccg gcagggatcg gccccagcag cg
//
LOCUS       DASRYE010000019        36417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_124652,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000019 DASRYE010000000
VERSION     DASRYE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 36417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36417
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124652"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFF98_00920"
     CDS             <1..974
                     /locus_tag="VFF98_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU62215.1"
                     /translation="SVYEIGQRMCDRFDDVPEGAEDSRLPHVFIAGDACHTHSPKAGQ
                     GMNVSMQDTFNLGWKLAAVLRGQCPPDLLRSYSSERRALAKNLIDFDREWAALLSAAS
                     KNDPANPVDPAQVEAYFVRHGHYTAGTATQYLPSQLTGPADHQALATGFPIGKRFHSA
                     PVVRLADARPMHLGHVVKADGRWRLFLFAPGGDQAAALARLEEACASLRADPASPIRR
                     FTPAGADVDAIFDLRCVLPFPHRGLAPTSLPELLLPAKGPLGLRDYEKAFCVDAAQDI
                     YALRGIDREQGCMVVVRPDQYVAHVLPFGDTAALGAFFGQIFLTPGV"
     gene            1066..2226
                     /locus_tag="VFF98_00925"
     CDS             1066..2226
                     /locus_tag="VFF98_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62216.1"
                     /translation="MKAGSWPSIILIYLFSVCAGGAIGKIIPLVGVLAQALHVGPDRA
                     AWLISAVLPASVVLAPFGGALSDRLGDRRLTAIGLAVMIAANGAQWLATGYGALLAAR
                     LVEGGGFVCLTLGPAAMMMRSTQGARQVAAMSLMSTALPLGVGLSLALAGQAPGARWQ
                     LVPLAHGLVLAALLPLLRLAPAGSGAPAPGTPPPLRWTELLRAPAPRRLAAGVLSASA
                     IMFGLGALYPTYAVAHFGIAPATAAAYGLVTYPASVIGSFMAGALARRHGLGRLGTGG
                     LVALALAGVAVFWPALGLVPSVLLLVGFYLLAGLVGAVGMARLPAVVPSPAAVGITTG
                     LYIQGANAGTMLGAPLLFAAYYGGAFPAVALAILACVALTLACWRFPAPSHR"
     gene            2400..3485
                     /locus_tag="VFF98_00930"
     CDS             2400..3485
                     /locus_tag="VFF98_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZU62217.1"
                     /translation="MGVGNTGYGVFPETDAVGLGVSALRAAVADAGIRFEDIDGVILN
                     RLPYDRFAEVSGLNTPWLQSTEAHGRFSAIALMMAAEALASGQATTIALVYGNDGRSN
                     AMKYGGGAGFWAPWGFTSPGASHAMMWRRHMARFGTTSADLGHIAVAFRDHASRNPQA
                     VMQKPISLEDHQNSRFICDPLHLLDYCLINDGGVAWIMTTPERAKDLRQKPVYVSGFA
                     RQDDFSQNSYAADDFWHPALQQVAAQVYDRAGVSREDIDGLMIYDNFSPTVIFALEGL
                     GFCPQGEGGRFVADGALQLGKGRWPTNTSGGHLSESYMQGWALIAEGVRQLRGEVPPA
                     RQIPDAKALQFCLATPVSASIILRTDA"
     gene            3482..3889
                     /locus_tag="VFF98_00935"
     CDS             3482..3889
                     /locus_tag="VFF98_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZU62218.1"
                     /translation="MTYAKPLPEADKWSGPFFAACREGRLVAQKCDATGRFFFPPAPV
                     SPFTRDEAWSWQEISPRGRIGSFVVMHQKYFAGFAGEVPYPVIEVELEGGVRLVSNIV
                     DLDGRALAVGMAVEAVFVKATDEVTLPLFRLVD"
     gene            3921..5414
                     /locus_tag="VFF98_00940"
     CDS             3921..5414
                     /locus_tag="VFF98_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU62219.1"
                     /translation="MMDLRDRLDPAAELPPHVRQRFYIDGAWRAPRGTERLHLVSPLS
                     EEVILSVPAGTPADMADAVAAAARAFAQGPWPAFTGPERAVFLRRMGQAVARRLDLFG
                     RVWTAQVGVPTTFAQMVLALVPRYFAYYADLAETCAFEDIRPTAQGHARVVREPVGVC
                     ALVLPWNAPLILLAQKLAAALAAGCTVVAKPSPETPLDALLLAECAGEAGLPAGVFNV
                     VPGAREAGEALVGDPRIDKVSFTGSTAAGQHIAALCAGRVARCSLELGGKSAAILCED
                     VDLAAALPALLPVAMPFSGQICFSQTRLLLPASRYDEMLEALVGAIDGITLGDPWEAG
                     TMMGPLAMARQRARVLGYIAQGRAEGARLLCGGDNGGFARGYFVAPTLFADVQPAMAI
                     AREEIFGPVLSVLRYRDEAEAIALANDSAFGLSGTVFTPDLERGTQIARAVRTGNISI
                     NTLQLDPAVPFGGFRQSGIGREGGPEGLAVFQETKAIYLPVLAPHTL"
     gene            5426..6208
                     /locus_tag="VFF98_00945"
     CDS             5426..6208
                     /locus_tag="VFF98_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU62220.1"
                     /translation="MSEEQLVVIEEKGTHAVLRINRPDKRNAMNVAMRQAMLAALEAL
                     KGRFAAIVITGTDLSFCAGLDLKEESALRAAGIAPDPASSWQAVNVAIRAHPAVFIAA
                     VNGIALGGGVTLINVCDLAIASDKAQMGMPEMGFATYPGLAGPSTQYSLPRKRHAWLM
                     FTTERIDGATAAQWGLVNKVLPQADLLAEAEAIAAKIGQFDPTALAISKAAMDRIPVE
                     IDWNGAFNFGVGEIERIRALSSSGDEGLARFSAGERLVGQGK"
     gene            6238..7704
                     /locus_tag="VFF98_00950"
     CDS             6238..7704
                     /locus_tag="VFF98_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU62221.1"
                     /translation="MREYQMYVNGAFRAAQGGRCLPTLMPWTGEAWALIPEASRADVD
                     AAVEAAHAALGGPWRDLPASGRGALLRRLADLLAAHAGHLAEIEVRDNGKLLSEMRAQ
                     MAYLPEVYYYYAGLADKVEGRVPPLARPGHLGFTRLEPVGVVAAITPWNSPLLLAASK
                     IAPALAAGCTVVHKPSEHSSASALEFARLVDEAGFPAGVFNVVTGLGPEAGDALARHP
                     KVARISFTGGTEAGRAINQIAAARFARCDLELGGKSPNIVFADADLDAAVNGVISGIF
                     AASGQTCLAGSRLLLEESIHDAFLDRLLEVARSATVGDPLDPGTCVGPVTTEAQLHKV
                     LAYIDIARKEGARCVLGGARLDRPGWFVAPTIFADVTNDMRIAREEVFGPVLSVLRFE
                     GEAEALRIANDCNLGLAAGVWTSDMGRALRMSERLEAGTVWINTYRMLSVTMPFGGYK
                     DSGTGRENGIEAICANLETKSVFLNHSAAVGNPFVMKL"
     gene            complement(7766..8983)
                     /locus_tag="VFF98_00955"
     CDS             complement(7766..8983)
                     /locus_tag="VFF98_00955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZU62222.1"
                     /translation="MTDLPLTGVSIVDFCWIGAGSYTTKMLADMGADVIKVESRRSLD
                     SLRLAAPYKDGIKGVNRSGYFADRNSSKRSITIDLKTPQGLELVHRLVADADVVANNF
                     RPGTMEKLGVGYAALRAINPGLIYAAMSMNGDEGPDRTMLGYGITIAAITSFLGLSGY
                     PDRPPTGTGTNYPDHVPNPCHGAFAIMAALRHKWRTGQGQQIDMAQTEPMLALMPLPV
                     MDYQANGRVAGRQGNRLAGHAPRGVYPTQGNDRWIAISIATDAQWQALAQILDGALLD
                     RPAWRTAPARAADADAIDTQLAALTRAQEGYGLMARLQAAGVPAGVVQNADDLVERDP
                     QLAHRQHWHYLPHAEMGSALYNGPPFRFASGPVGPRFAAPLLGEHTREILAGRLGLAD
                     AEIDRLIAAEVLA"
     gene            complement(8980..10140)
                     /locus_tag="VFF98_00960"
     CDS             complement(8980..10140)
                     /locus_tag="VFF98_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZU62223.1"
                     /translation="MADLQADPRALAGLRVLELSGLSGAYCGRLFAGLGAAVTLVEPP
                     GGSPMRAVAPFAGDAPGPENSLSFAYYAAGKASLVLDLETAPGRAALHRLAGKADLLI
                     LADHPQAAAVDGAGLCRDHPRLVALRITPYGSSGPYAGFVGDDLTLMAMGGLLTMAGF
                     PGEPPVVAHGGQGLLAADQFAAVAALAALLRAERTGRGELIDVSIQEAIVMALENAAQ
                     TYQLEGRIRQRAGTAARAGTGIYPCRDGEIYLLAGGIGETAMWGHFARWMADEGIAGA
                     ERFTAADWDDTTPAACAAFEALFLPYAARHTKAALYARARAWRVPMAPMSTPVDLLEN
                     PQLSHRGFFVAPGQGPTAGLRMPGAPYKLSATPWRTGPAPALAPAPVIEELP"
     gene            complement(10144..11352)
                     /locus_tag="VFF98_00965"
     CDS             complement(10144..11352)
                     /locus_tag="VFF98_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62224.1"
                     /translation="MNFNLSDEARMIGEMTARFVADELIPLERTVIEREAARGLGGDA
                     LIPPEAEARLNARSKELGLWGIDVEERFGGHGMGALVKCIVTEQMNYSITPFILPPDS
                     PNLQFLQECCKGDQIERYLLPYARGEKKSALALTEASAGADASGIRLKAERRGGKWLL
                     NGEKMWISHARTADFMIVLGVSDKDKGTRGGMTAFLVDKGTPGVTVPQDFSVIGGNFH
                     GYAVNFDDVELDDGQVLGEVGWAFAPLQNRLGIRRMECACWCLGYAQRALDLMIEQAR
                     NRVTFGQPLAERQTVQNWIADSYQEIEMVRMMTYRLAWLLDNPGDDERRSAVRIAAST
                     VKVQATEMATRVVDRAMQCLGGMGLCKELPLEYMFREIRVLRVVEGPSEIHRWLIARE
                     LIRDGRPRDF"
     gene            11498..12448
                     /locus_tag="VFF98_00970"
     CDS             11498..12448
                     /locus_tag="VFF98_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU62225.1"
                     /translation="MTQSRLPPLTALKTFECTGRLLSFTLAAGELGVTPGAVSRQIKV
                     LEDYLGLQLFIRSGREVSLTKAGGTYLAQLTEAFARIRAATSELIGVPEDAPLRVSSS
                     VTFTMRWLMPRLMSFHSRHADSNLQLTMNLAPVDFQRDDLDATIKLGYEDTPHSVVRR
                     LFRADLVPVCSPRLIDQPGGPADYDWLGRHTLLHSSARPDNWLVWLRSVGRPELKGAR
                     QLQFESSSLAYQAAIEGVGIAMAQLPLVLEDLRTGALVLPFPVAVRDTEVYNLIWPDR
                     TPRNRGFLPFRDWILAEAQRTEAEIVDVLARLESGRAGPL"
     gene            12584..13558
                     /locus_tag="VFF98_00975"
     CDS             12584..13558
                     /locus_tag="VFF98_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU62226.1"
                     /translation="MLLDGKVALVTGAGRGLGREHALALAAAGAAVVVNDLGGSNRGG
                     GADAGPAQAVVAEIEAGGGRAIASTSSVSDWAAAGEMVQAAIAAFGTIDIVVNNAGIN
                     RVGLLGEISEADWDLSADVIAKGTLATMNAAVAHWKQAGPKAGRAIINTASPAALHPA
                     PGIGAYCAAKAAVVALSQSAALELAPLGVRVNAIAPMARTRMLEGAPQELLDLMAPRD
                     GFDPNLPEHVAQLVVYLAGPACRFTGRVFGSWGDQLFLFREWDAAFQAGNGNCKWTQE
                     RLATALADFPLTDRRHILIPGGRMEADMPPEPLLALLGQAAAAVVERT"
     gene            13555..14334
                     /locus_tag="VFF98_00980"
     CDS             13555..14334
                     /locus_tag="VFF98_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZU62227.1"
                     /translation="MSAIEIPTAPRRPGEGVRLFAFTVGHVHLPLAFFLEGEAGTICA
                     PVTAFLIDHPTGLALFDTGLGPRFARPPGHPREGFIDLEDGQTMAQSLRKIGVEPEDV
                     RWIIASHFHADHAGGNAQFPNACVIVHAAEVAHAHAMADGDLYCPAEFDTGQPLLRLD
                     GEHDVFGDGTVVVFPTPGHTPGHQSARVQTDGGDIVLTGDCCNMKRSLDELRLPDHCH
                     DAERSLASLRVLRGLQDKGARVFPSHDPQFWAGIAMNVPIR"
     gene            14470..16095
                     /locus_tag="VFF98_00985"
     CDS             14470..16095
                     /locus_tag="VFF98_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU62228.1"
                     /translation="MGEFDYIVVGSGSGGGVVAARLSEVPGVSVLVLEAGGADWNPVF
                     HMGIGFYRNRPAGLNWGFASEPEAHLENRSVDLPQGHVLGGSSSINGKIYSRGHPVDY
                     DQWRQLGCEGWSFAEVLPYFKRAEGSWRGEGPYHGGRGPLKTAPVDARGLLQEELLAA
                     AQSVGHAVVDDLDAAPFGGLSRRSDITVGPRGRRSSTARAYLRPARHRRNLTVLTRAR
                     ATRILFEEGRAVGIAYQRGGQAHVVRARKEVILAGGVYNTARLLLLSGVGPADELADI
                     GIAPLHDLPGVGRNIADHASYMLGFETQGRVSAIRQLRYDRAAFHFLRWLLAGTGLFA
                     TQPLMGQGLFKTMPGLDRPDIQVFFNPIRRDVQLWFPGIRAEQQHRLEAGLILLHPHS
                     RGSLQLASADPHEAPKISLNLFSDPRDVETMLRGVRLVREIYAASPLRDLLRAEVAPG
                     EHLQSDAEIIAALRRGLFTVRHPVGSCRMGTDAMAVVDPQLRVRGLAGLRIADASIMP
                     AIVGGNTNAPTIMIGEKAADLILGRPPLPPAAL"
     gene            16175..17320
                     /locus_tag="VFF98_00990"
     CDS             16175..17320
                     /locus_tag="VFF98_00990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU62229.1"
                     /translation="MGQIWQAQLDHFKAMIEQDIAKGLYYGAAVRVARAGRLVFDETL
                     GHADGAGTQALDRRSVFSIFSMTKAFTNVLILRAIEQGRFALTTKVSEIIPEFAGPPR
                     NEATFFHLLTHTSGWPAVWEVEPEAYQGDFDAVLAGVIARVHGVIAPGQRCDYQPMVN
                     HTLMGEALRRTDPRGRALRDIYREDLFAPLAMHDTALGVRADLQPRHVVPDMRGTLPV
                     ALPGRTKPGRYAMFEEEEAEMPHVGCVSTADDIHRFAEMLRRGGELEGTRILSPRTIA
                     LARRNWTGEMENELYRGLAMSMGARGYPAYIGLGFSLKGDAVVPGQYGTLTGVGTFGN
                     NGAGSTLFWVDPQADATFVCLTAGVMPEWENIKRFQRYSDAVAAAMV"
     gene            17335..18132
                     /locus_tag="VFF98_00995"
     CDS             17335..18132
                     /locus_tag="VFF98_00995"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU62230.1"
                     /translation="MLTYDYKGKVVLVSGAGSGIGRAIAEAFGAAGASVAVADIAPAS
                     AEATAAGIAAAGGTARAFTVDVADEAAAAALVADIVAAFGRLDCAINNAGIEAANLPL
                     AELDSAIWRRVSDVNLSAVFYAMKAQIPQMLTQGGGAIVNTASISGLIGGYNLAAYTA
                     TKHGVIGMTKAAAMDYADTDFTGQGGIRINALCPGLVDTPFIAQLPPGLRKRLIDGIP
                     MRRPARAAEIARAVLWLCSADASYVTGHAMVVDGGASLGGEATRFDD"
     gene            18161..19345
                     /locus_tag="VFF98_01000"
     CDS             18161..19345
                     /locus_tag="VFF98_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU62231.1"
                     /translation="MSALVTEDLPRISANPTDAEYAEARRHGWLATSERGYEVLAYKQ
                     GWEVMSSKAFGIGGGFGNILDQLGLTEGPYRDEWNKIIVCTEHDLRDHMRKPYMHLLK
                     PQQVAKLQGLVRELIHGILDDIKDPTDVDFMKEIGDRLPTQLYCELISAPRAMAPEIY
                     RITNSINPPLITFNKARLKESEEAYWEGMAFLTEHVEARRNNLGDDFTSELIRCEQAG
                     MMRPDETMSVAMSLLIASMDNTMHQLGLSFGTLLEDRSRWEQVLARPSAAPQAAEETF
                     RLCPRFNVITRHAPEDVEMDGFVFPAGSWVMVATRSTGRDEAAYDTPDSFRLGRPAGK
                     ALQFGGSSFSCLGATLARLEISETIRIIAERFPGIRMIGDWHKAVGPMVTECEQLRVS
                     LV"
     gene            complement(19242..20588)
                     /locus_tag="VFF98_01005"
     CDS             complement(19242..20588)
                     /locus_tag="VFF98_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZU62232.1"
                     /translation="MRAISRAISRQGWVMLHRWAGLSTALFLAVAGLTGMALAWEEEI
                     EGWTAPQLLRAAPAHPGQPLRDPAELARAALARHPGMAITYLPLSVPPGRSLDIRVSW
                     PGLAHPPPADELFLNPYTGAELGWRHWGDIGEGWRNLMPFVYRLHYSLLLGDAGELVM
                     GIAALVWMGDCFLGFYLTLPPPAPRGAARKGSWLASKVGWLARWRPAWVVRRHVSAHK
                     RHFDLHRAGGLWVWPVLLVFATSSVSFNLPGVYGPVQRALGGQDGAALLAARALPAPR
                     LHPALDPVAARARGAALAAAAIARAGAQGRPQGASWLWQAPAAGAWIYGFSTSADIPD
                     KGGASRLAFDSDTGQLLGVALAGQAPAANRFTDWIVALHMARVFGPWWQALVSLVGAA
                     VCLLAATGVLIWLKKREARRRRIRPATPAAARIPSPSAPPPCASRQSCECRESARR"
     gene            complement(20596..22773)
                     /locus_tag="VFF98_01010"
     CDS             complement(20596..22773)
                     /locus_tag="VFF98_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZU62233.1"
                     /translation="MRDPFVRAYALPLAGISLLALCAHPALAADAADASTTAADTADA
                     DTAGILVMGHQSAAPVTSATKSSLPIAETPQSISTISAQDIANLGLANLNQSLRYVAG
                     ITPETRGANAEIYDLFKLRGFDAPVYLDGLKQFSSATGYAVPQVDLSRLENIEVLKGP
                     ASALYGQSSPGGLVAMSSKLPLDKDFYGSVAGTYGTYNLYRIDADVGGKLSDAIGVRL
                     YASANGAHTQQSFGARERQTISGAITAKLDARTTLTVLAAYSHDPKNGNYGGAPASGT
                     LFANPNGRISTSFADGEPGDFFRREQAGVTYILAHDLGGGWAVRASGRYQYVGSNLGA
                     IYTTGTVTDPAQTTFARGSYATHETLHNWTFDNQLSGHFTTGPVSHSLLVGVDYQAAH
                     SFEAAGFGSATAINAYAPTYGTQAVPQTPADAGTVYTYDYHQYQTGLYAQDTMSWGGL
                     RLLLSGRHDWARTNDGSSSQSDAKFTGRAGLLYATGLGLAPYASYATSFEPQSGSVLH
                     ADGTIGNAKPSFGKQLEFGLKYQPKGTGILVTAAWFHIEQSNILTAIPNSVYSTQSGK
                     VRSEGFEIEGHVPLPAGFALRAAYGHQKVRTIADAETPANVGQGLIGSGDGNLALNLD
                     WAARGGPLKGLTLGAGLRHVENVYAGAVDGVATYTPAYTLVDALMRYDLGATHPRLAG
                     LDVSLNIANLFDKTYLTSCYLSGVGWCWYGQRRTVQGTLRWHW"
     gene            23092..24660
                     /locus_tag="VFF98_01015"
     CDS             23092..24660
                     /locus_tag="VFF98_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU62234.1"
                     /translation="MPHYDCVIIGSGINGLVCAAMLGKKGLRVAVLERADVAGGCIRT
                     GEITLPGYVHDMLSGFHPLFVSSPAYAALGADLHARGLAYSNAALPTGVLLPDGRWTV
                     MARDRAANIARFEALAPGDGAAFDRAMQAVAADAPLTFALLGEDLWRPAMARKLAGAA
                     WRKGAGGMIAYAGAALASARDWLEADFASDELRALFAPWVLHTGLGPDQAVSGYMAQV
                     IAFALEAAGMPVVVGGSARLVEAFVALIETQGGAVLCGAEAAGIRVAQGRAQGVDLAD
                     GRHFAASKGVFASVTPQALYGRLLPPEAVPVAVARAAQAFRYGRADMQIHLALDRPVA
                     WPAAELADTAMVHLTGGIDDVAQAVAQANAGLLPARPTVVVGQPTAQDPSRVPQGKGL
                     LWLQLQELPRVPRGDALGLLDGTQGWAPGLRDAYADRVMAHVERFLPGLGASVLARQV
                     LGPAELEALNCNLVGGDPYAGDCALDQSFLWRPGAAMRNHHTCVKGLYHIGASTHPGP
                     GLAGTSGFIAAGYL"
     gene            complement(24671..25423)
                     /locus_tag="VFF98_01020"
     CDS             complement(24671..25423)
                     /locus_tag="VFF98_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZU62235.1"
                     /translation="MALIRMILPVPLPDAALAHFAAQIPQHLLRPDMRLEFRGCRAGA
                     SLLDSPCEWSLADAFVLDAGLDAEAAGCDAVCSFTLSDSGLAGLRSCLAIPVVGAGQA
                     ALSLAAQLGRRFSILTMWQPWQQLARETAAKHGLADRLASVRHIATRPDTHELLAGKE
                     EIVFARLEAEARRAIAEDGADVIVLGSTTMHQSHAYLAACLPVPVINPGLAAYKACET
                     LIDLKLAHSKAAWPAPERRPDGLFAPIPALFA"
     gene            complement(25489..27795)
                     /locus_tag="VFF98_01025"
     CDS             complement(25489..27795)
                     /locus_tag="VFF98_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62236.1"
                     /translation="MRHTKRVWLFTASALALAPQLALADTPAASTDNGSGDIVVTAQR
                     REQNLQDVGIAISAFSGDTLKKMGVQSSTDIARLTPGVSISASGGGQDSQFSIRGVTQ
                     NDFSDAIEAPVAVYVDETYIPNLNGQIFGTFDLQRVEVLKGPQGTLFGRNATGGLVHF
                     IPVKPSDKPNAYAEVTYGAYNHVRVEAAIGLPLSDTVQFRVSGLYNRNTGYLKNLYPA
                     GTSSLTAGSTAPQGGAGADLGGENSKAVRAQLQFEPTDRLTVRLTGSYLAQHLSVNPY
                     NSTGTRAVYDSQGRVVNVVFANGDSYGYNAPGKQSLEVSSDWSIPDRNRLFAWDTAVH
                     INYQLDGAEIVAISDYRHFRKDYGTDVDAGPENLIDFISDSVEHSFSQELRIAGTKKG
                     PVRWVAGAYFLDINSLVHNGFKAPSNSVFAAYYPGYAQSGIDLINDVNLKTESISAFA
                     QGEYDFAPKLTLILGGRIIHEHQDYTFDSMAHANTNDYEFTYGPALFQLQDPYANKRN
                     TLMWAGKAQVEYRPAKGTLLYFGVNRGVKGGAYNAQLPDGSASLSPSRIAYNPETLVA
                     YEAGLKTGFWHNMLTLDLSGYYYDYHNYQAFTFSNVSGIVSNNDARNFGMEASLNLRP
                     ARGLNIGLGLSLIDAKVKHVAIATGIYKDVRPTFTPAKTLAAQIGYTLPEQLAGGDIN
                     FNIDGSWRSKVYANIRNFDADIINPYFLANMHVYWNRGPLRLGIGVENMFDERYKTVT
                     YDLATLCGCNEESYGMPRWWNVSLRYTY"
     gene            complement(28001..29140)
                     /locus_tag="VFF98_01030"
     CDS             complement(28001..29140)
                     /locus_tag="VFF98_01030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU62237.1"
                     /translation="MTQTANRMRSKHFKLGTFSSNCSGGMSITNVPERWKASWDENLA
                     LARMVDEAGLDFMLPVARWIGYRGGPSNFQSGVLETMTWAAGLLASTRNIAVIATVHA
                     AAFNPVVAAKQIVTLDQIGHGRAGLNIVAGWNKPEFDALGLNLPDDHESRYAHAQEWF
                     DVLKKLWTSTEPFDFDGSFFRLRGVWSDPLPFNGLPPIINAAGSAQGRAFATRNADYL
                     FTPAIDLARSAPEIEQLKGAAQANGREVEVLTFSHVVCRPTEAEAKAYLQHYAHDYRD
                     VGTLEEVIALQFAHAHSFPHDLLTLIKDRFAAGHGGYPLIGTPEQVADGICALHETGF
                     AGTTLSFVDYVAEFPFFRDNVLPLLEARGLRISDTSSARAPALVG"
     gene            29420..30586
                     /locus_tag="VFF98_01035"
     CDS             29420..30586
                     /locus_tag="VFF98_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZU62238.1"
                     /translation="MAETILIPRTGAVAADDPACFNRLHWAIAPVARARPGDTIIYET
                     RDAFDHQFTPTSPHAAAATVDGRRVHPMTGPVHIEGVERGDALAVTIVDIEPDSYGYT
                     SVVPGFGFLRDVIPGPFLANWKLDRLSATSEQMPGIRVPMAAFPGSIGVLPGVPETAD
                     ALAREGALAEAGGFAPGPEPAEAVPASLFGPGAPYEKEGLRTFGPRECGGNMDVKAMQ
                     VGTTILFPVQVDGAGLWTGDIHYAQGDGEVCGTAIEMDARVTLRAEVIKGGGRNMRFP
                     HIIGGNQLARVAPGRFYAVTGLPVKKAGELPPHLAWLGSAKLAALTNVSEDLTLATRD
                     ALIRIIDWMVENKGLSREQAYVLASVAVDLRIGQLVDVPNFVVTAVLPLDVFED"
     gene            30604..31797
                     /locus_tag="VFF98_01040"
     CDS             30604..31797
                     /locus_tag="VFF98_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62239.1"
                     /translation="MNLQHRWAEANPWLGTGPIPVEPYISPAYYEKEKEKIFKRVWLC
                     VAREAEVPQPGDYKVKRIAAGDTSAIIVRGKDGKVRAFHNMCSHRGNTVVTETGEETY
                     GASRAGVMTCRFHGWVYGADGALKQVPQEGRFFACFDKADNGLAPIHCDIWQGFVFIN
                     LAAQPEESLMSFLGGYADHVAGYDFSALTYHYHYHTMLNCNWKVAHDAFAEAYHVDTI
                     HAGSFPNTFSSGIQNVKLMGPHRTCAVCLTLGANPTPIGTLAAEKARGSLTLRAAESR
                     LPPTINPDRRSDFAFELSVLFPNTLVHVSDGIWFTHQFWPVAHNQTLWEGRYYVQTPQ
                     THSARWALEQAIILQRNAWLEDTATMEDTHRAMQSGAKQVQFLQDDEILIRHGAAVVD
                     QYVQA"
     gene            31808..32182
                     /locus_tag="VFF98_01045"
     CDS             31808..32182
                     /locus_tag="VFF98_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62240.1"
                     /translation="MSEIILPPGFEALAVHAEWALDTPDERHRARLASTPARLEAFYN
                     GILPWLEKILEELDRFPLGALPESHHALFNLAFAVIEVAPHVELYRSSPGVPFAFEEL
                     RFVAAHGGQPTWAGRPPAEMRR"
     gene            32179..32682
                     /locus_tag="VFF98_01050"
     CDS             32179..32682
                     /locus_tag="VFF98_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62241.1"
                     /translation="MNEARMAAMEQRLARLEAVEAICAVVARYAIAADLHNDPALFGA
                     LFAQRAVWEARGFGRHEGRAAILAALAEVGRQTMLWTTHFNVAPLVEPAADLASARCR
                     WYLWELARMADGADGADGAADHWIAGTYDARLIVEGGAWRIAELQLDLRLMTPVAAPA
                     FAPQVAA"
     gene            32679..32993
                     /locus_tag="VFF98_01055"
     CDS             32679..32993
                     /locus_tag="VFF98_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU62242.1"
                     /translation="MSDLRIALATLDADGITCIAGPAGAALVVCLVEGAYYVTDDLCS
                     HGDASLAEGWLEGFDLVCPYHSGAFDVRTGAASRAPCALPVRSYAVRREGEELVIGPA
                     QG"
     gene            complement(33005..34027)
                     /locus_tag="VFF98_01060"
     CDS             complement(33005..34027)
                     /locus_tag="VFF98_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZU62243.1"
                     /translation="MAAAPHRDALAGALQGLYQDPGRAFRTHDPDHVRLHMSQVFCRH
                     SLQVRAEPKGFGFSHTETPLRQLSFNTTIYDGRDGEVWCDIAPCDNIHLIQFSLAGTA
                     EIRHAGAEVLLRPGEMCALDLSLGAKVRFNADYRHFTIKIPTTALTQLIAQDEGRNCP
                     PLQFTPHAVPLVGQAAALGNFVSLLCSEMQGPERGLTHSRMIDTTEQMLMRLLLLSLP
                     NNYYDHHQPAAGPAIPYYIRRAEDFMRANLREPVTLDAIIQAAGISSRSLQAGFRRHR
                     QDTPLGYLKKLRLDSAHRALRAGRGNVTQIALETGFTHLSKFARDYRERFGETPSETL
                     MRGASK"
     gene            34238..34747
                     /locus_tag="VFF98_01065"
     CDS             34238..34747
                     /locus_tag="VFF98_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU62244.1"
                     /translation="MALTNPDAAQPAEAAPASEDHKAGSLDLRLWIRLLGSAKLIEKR
                     LRRNLQDQFGTTLPRFDVLATLERTGQGMTMGALSRALLVSNGNVTSIVRQLHESGMV
                     AVVADPDDRRSAIVSLTPHGRAQFAIYARAHRGWVQEALAGFAPARQRQLFELLGALR
                     ATLRAQDGD"
     gene            complement(34754..36190)
                     /locus_tag="VFF98_01070"
     CDS             complement(34754..36190)
                     /locus_tag="VFF98_01070"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HZU62245.1"
                     /translation="MNESKRIAIAILALGGQGGGVLAEWIQHMARAAGWIAQGTSVPG
                     VAQRTGSTVYYVELARPGADGRAPVMAQMPMPGDVDVVIASELMEAGRALLRGFADKR
                     TTLIGSTHRIYAISEKQVAGDGRGNSAKILAAASERTARFIGFDMAAATIASGSVISA
                     VMFGALAGSGALPFPRAAFEAAIRASGKAVPSNLAGFEAGFAGAGGPLALPAEADSPP
                     PQPTTEAGRALAERAAQSLPAAALPFALPFVLHGLARLMDYQDAAYAALYLERLAAIA
                     ALDAAPFELTRETARHLALWMAYEDTIRVADLKIRASRLERVRAEVRAQSGQILTLTE
                     FFHPRLREVAETLPAGLARRLLASPALSRWLERQLAGGRLAQTTSLRWFLALRLVAAG
                     RRWRRRTLRYGEEQARIEVWLARMASQARHDLALATETARCQRLIKGYGDTFERTLAQ
                     FEAVMAMPAPTAAALAEARSAQLASCPA"
     gene            complement(36204..>36417)
                     /locus_tag="VFF98_01075"
     CDS             complement(36204..>36417)
                     /locus_tag="VFF98_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62246.1"
                     /translation="QDPVSHVDDSCVGCGLCGENAHAAVLCPSFFRARIVTHPARWEK
                     LRARWRQRLTGWLQARDEQARLARAF"
BASE COUNT         5639 a        11869 c        12876 g         6033 t
ORIGIN      
        1 ggtcggtcta tgagatcggc cagcgcatgt gcgaccgctt cgacgatgtg cccgaggggg
       61 ccgaggatag ccgcctgccg catgtgttca tcgccggcga tgcctgccac acccacagcc
      121 ccaaggccgg gcaggggatg aatgtgtcga tgcaggacac cttcaacctg ggctggaagc
      181 tggcggcggt gctgcgcggc cagtgcccgc ccgatctgct gcgcagctat tccagcgagc
      241 ggcgcgcgct ggccaagaac cttatcgatt tcgaccgcga atgggccgcc ctgctgagcg
      301 ctgcctcgaa gaacgatccg gccaatccgg tggatccggc gcaggtggag gcctatttcg
      361 tgcgccacgg ccattacacc gccggcaccg ccacgcaata tctgccctcg cagctgaccg
      421 gccctgccga tcatcaggcg ctggccaccg gctttcccat cggcaagcgc ttccattccg
      481 ccccggttgt tcgcctggcc gatgcccggc cgatgcatct gggccatgtg gtgaaggccg
      541 atgggcgctg gcggctgttc ctgtttgcgc cgggcggcga tcaggctgca gcgctggccc
      601 ggctggagga agcctgcgcc tcgctgcggg ccgatcccgc ctcgccgatc cgccgcttca
      661 cgccggcggg cgcggatgtc gatgcgatct tcgacctgcg ctgcgtgctg cctttccccc
      721 atcgcggctt ggcgcccacc agcctgcccg agctgttgct gccggccaag ggcccgctgg
      781 gcctgcgcga ttatgagaag gccttctgcg tggatgccgc gcaggacatc tatgccctgc
      841 gcggcatcga ccgcgagcag ggctgcatgg tggttgtccg gcccgatcaa tatgtcgccc
      901 atgtgctgcc ctttggcgac acggcggcac tgggcgcctt cttcgggcag atcttcctga
      961 cgcccggcgt ctgaccaacc accccggcgg cgcaatcgac gcaaacagcg cgggcgccgc
     1021 cggatccgca caggctgcgg atcagccaaa ccacgggaga atgtcatgaa agcgggcagt
     1081 tggccgtcga ttatccttat ctatctgttt tcggtgtgcg cgggcggggc gatcggcaag
     1141 atcatcccgc ttgttggcgt tctggcgcag gcgctgcatg tggggccaga cagggccgcc
     1201 tggctgatct cggcggtgct gccggcctcg gtggtgctgg cgccctttgg cggcgccctg
     1261 tctgaccggc tgggcgaccg gcggctgacg gcgattggcc ttgccgtgat gatcgcggcc
     1321 aatggcgcgc aatggctggc caccggctat ggcgctctgc tggcggcgcg gctggtggaa
     1381 gggggcggct ttgtctgcct cacgctgggg ccggcggcga tgatgatgcg cagcacgcaa
     1441 ggcgcccggc aggtggcggc gatgtcgctg atgtccaccg cgctgccgct gggggtgggc
     1501 ctgtcgctgg cgctggcggg gcaggcgccg ggcgcgcgct ggcagctggt gccgctggcg
     1561 catgggctgg tgctggccgc cctgctgccg ctgctgcgcc tggcgcctgc cggcagcggc
     1621 gcgcccgccc cgggcacgcc gccaccgctg cgctggacag agctgctgcg tgcgcctgcc
     1681 ccgcgccgcc tggccgccgg cgtgcttagc gccagtgcca tcatgttcgg cctgggggcg
     1741 ctgtatccca cctatgcggt ggcgcatttc ggcatcgctc ctgccaccgc cgcggcctat
     1801 gggctggtga cctatccggc ctcggtgatc ggcagcttca tggccggggc gctggcgcgg
     1861 cgccatgggc tggggcggct gggcacgggc gggctggtgg cgctggcgct ggcgggcgtg
     1921 gcggtgttct ggccggcgct ggggctggta ccatcggtgc tgctgctggt gggcttctat
     1981 ctgctggccg ggctggtggg cgcggtgggc atggcccggc tgccggcggt ggtgccatcg
     2041 ccggcggcgg tggggatcac caccggcctt tacatccagg gcgccaatgc cggcaccatg
     2101 ctgggagccc cgctgctgtt tgccgcctat tatggcgggg cctttcctgc cgttgcgctg
     2161 gcgatcctgg cctgcgttgc cctcacgctg gcctgctggc gctttccggc accctctcat
     2221 cgttgatctc agctcaagag tgcgcgcctc tttgtggttt gacttgaggg cccgccggca
     2281 accaattggg cccatcgcca caaggcgcac acacggatcg ggcgggcctc tggcaggcgc
     2341 cccggcgggg atgaggaggg catatgccgg attggaatct gcgagacagg gcgtgtatcg
     2401 tgggggtggg caacaccggc tatggcgtgt ttcccgaaac cgatgcggtg gggctgggcg
     2461 tttcggcgct gcgggcggcg gtggccgatg cgggcatccg ctttgaggat atcgacgggg
     2521 ttatcctcaa ccgcctgcca tacgaccgct ttgccgaagt gtccgggctg aacaccccct
     2581 ggctgcaatc gaccgaggcg catggccgct tttccgccat cgcgctgatg atggcggccg
     2641 aggcgctggc ctcgggccag gccaccacca ttgcccttgt ctatggcaat gacgggcgtt
     2701 ccaacgcgat gaaatatggc ggcggcgcgg gcttctgggc gccctggggg ttcacctcgc
     2761 cgggggccag ccatgcgatg atgtggcgcc gccacatggc ccgctttggc accacatcgg
     2821 cagatcttgg ccatatcgcc gtggcctttc gcgatcatgc cagccgcaac ccgcaggcgg
     2881 tgatgcaaaa gccgatctcg ctggaggacc accagaacag ccgcttcatc tgcgatccgc
     2941 tgcaccttct ggattattgc ctgatcaacg atggcggcgt ggcctggatc atgaccacgc
     3001 ccgaacgcgc caaggatctg cgccagaagc cggtctatgt ctcgggcttt gcccggcagg
     3061 atgatttctc gcagaattcc tatgccgccg atgatttctg gcaccccgcc ctgcagcagg
     3121 tggcggccca agtctatgat cgcgcaggcg tgtcccgcga ggacatcgac gggctgatga
     3181 tctatgacaa tttctcgccc acggtgatct tcgcgctgga agggctgggc ttctgcccgc
     3241 agggcgaggg gggccgcttt gtggccgatg gcgcgctgca actgggcaag ggccgctggc
     3301 ccaccaacac cagcggcggc cacctgtcgg aaagctatat gcagggctgg gcgctgattg
     3361 ccgaaggcgt gcgccagctg cgcggcgaag tgccgcccgc ccggcagatc cccgatgcca
     3421 aggcgctgca attctgcctt gccacgcctg tctctgcctc catcatcctg aggaccgacg
     3481 catgacctac gccaagcccc tgcccgaagc cgacaagtgg agcggcccct tcttcgcggc
     3541 ctgcagggaa ggccggctgg tggcgcaaaa atgcgatgcc acgggtcgtt tcttctttcc
     3601 gccggcgccc gtctcgccct tcacccgcga cgaggcgtgg agctggcagg agatttcccc
     3661 gcgcggccgg atcggctcct tcgtggtgat gcaccagaaa tatttcgccg gtttcgccgg
     3721 ggaggtgccc tatccggtga tcgaggtgga gctggaaggc ggcgtgcggc tggtgtcgaa
     3781 catcgtcgat ctggatgggc gcgcgctggc cgtgggcatg gcggtggaag cggtgttcgt
     3841 gaaggcgacc gacgaggtga cgctgccgct gttccgcctg gtggactgaa ccgccccgca
     3901 accataacaa caggagagcg atgatggacc tgcgagaccg gctggacccg gccgccgagc
     3961 tgccgcccca tgtgcgccag cgcttctaca tcgacggggc ctggcgggcg ccgcggggca
     4021 cggaaaggct gcacctcgtc tcgccgctga gcgaagaggt gatcctctcg gttccggccg
     4081 gcacgcccgc cgatatggcc gatgccgtgg cggccgctgc cagggccttt gcgcaagggc
     4141 cctggccggc cttcacgggg ccggaaaggg cggtgttcct gcgccggatg gggcaggcgg
     4201 tggcgcgccg gctggatctg ttcggccggg tgtggacagc gcaggtgggc gtgcccacca
     4261 cttttgcgca gatggtgctg gcgctggtgc cgcgctattt cgcctattac gccgatctgg
     4321 ccgaaacctg cgcctttgag gatatccgcc ccaccgcgca aggccatgcc cgggtggtgc
     4381 gcgagccggt gggcgtgtgc gcgctggtgc tgccgtggaa cgcgccgctg atcctgctgg
     4441 cgcagaagct ggcggcggcg ctggcggcgg gctgcacggt ggtcgccaag cccagcccgg
     4501 aaaccccgct cgatgcgctg ctgctggccg aatgcgccgg ggaggcgggg ctgccggcgg
     4561 gcgtcttcaa tgtcgtgccg ggggcgcgcg aggcgggcga ggcgctggtg ggcgatccgc
     4621 gcatcgacaa ggtgagcttc accggatcga ccgcggctgg ccagcatatc gccgcgctat
     4681 gcgctggccg ggtggcgcgc tgcagcctgg agttgggcgg caaatcggcg gcgatcctgt
     4741 gcgaggatgt tgatctggcc gccgccctgc cggcgctgct gccggtggcc atgcccttca
     4801 gcggccagat ctgcttttcg cagacgcgcc tgctgctgcc cgccagccgc tatgacgaga
     4861 tgctggaagc gctggtcggc gcgatcgatg gcatcacgct gggcgatccg tgggaggcgg
     4921 gcacgatgat gggcccgctg gccatggcgc gccagcgcgc gcgggtgctg ggctatatcg
     4981 cccagggccg cgccgaaggg gcccggctgc tgtgcggcgg cgacaatggc ggctttgccc
     5041 gcggctattt cgtggcgccc acgctgtttg ccgatgtgca gccggccatg gccatcgccc
     5101 gcgaggagat cttcggcccg gtgctctcgg tgctgcgcta tcgcgatgag gccgaagcca
     5161 tcgccctggc caacgacagc gcctttggcc tgtcgggcac ggtgttcacc cccgatctgg
     5221 agcgcggcac gcagatcgcc cgcgcggtgc gcaccggcaa tatcagcatc aacaccctgc
     5281 aactggatcc ggcggtgccg tttggcggtt ttcgccagtc gggcatcggc cgggaaggcg
     5341 ggccggaagg gctggcggtc tttcaggaga ccaaggcgat ctatctgccc gtgctcgccc
     5401 cccacaccct ttgaggaaga acgccatgtc tgaagaacaa ctcgtcgtca tcgaggaaaa
     5461 gggcacccat gcggtgctgc gcatcaaccg gcccgacaag cgcaatgcca tgaatgtggc
     5521 gatgcgccag gccatgctgg cggcgctgga agcgctgaag ggccgctttg ccgcgatcgt
     5581 catcaccggc acggatctgt cgttttgcgc cgggctcgat ctcaaggagg aaagcgccct
     5641 gcgcgcggcc ggcatcgcgc ccgatccggc cagctcgtgg caggcggtca atgtggccat
     5701 ccgcgcccat ccggcggtgt ttatcgcggc ggtgaacggc attgcgctgg gcggcggggt
     5761 cacgctcatc aacgtgtgcg atctggccat cgcttccgac aaggcgcaga tgggcatgcc
     5821 cgagatgggc tttgccacct atccggggct ggcgggccct tccacccaat acagcctgcc
     5881 gcgcaagcgc cacgcctggc tgatgttcac cacagagcgc atcgatggcg ccaccgccgc
     5941 ccagtggggg ctggtgaaca aggtgctgcc ccaggccgac ctgctggccg aggccgaggc
     6001 gattgccgcg aagatcggcc agttcgatcc caccgcgctg gctatcagca aggcggcgat
     6061 ggacaggatt ccggttgaaa tcgactggaa tggcgccttc aacttcggcg ttggcgagat
     6121 cgagcggatt cgcgccctct cttccagcgg agacgagggg ctggcccgct tttccgccgg
     6181 cgagcggctg gtgggccagg gcaaataagc aggccaataa acgggagcag ggcgccgatg
     6241 cgcgaatatc agatgtatgt gaatggcgcg tttcgggcag cgcagggcgg gcgctgcctg
     6301 cccacgctga tgccctggac gggcgaggcc tgggcgctga tccccgaggc cagccgcgcc
     6361 gatgtggacg cggcggtgga ggcggcccat gcggcgctgg gcggaccatg gcgcgatctg
     6421 ccggccagcg ggcgcggcgc cttgctgcgc cggctggccg atctgctggc cgcccatgcc
     6481 ggccatctgg ccgagatcga agtgcgcgac aatggcaagc tgttgagcga aatgcgcgcc
     6541 cagatggcct atctgcccga ggtttactac tattacgccg ggctggccga caaggtggag
     6601 ggccgcgtgc cgccgctggc ccggcccggc catctgggct tcacccggct ggagccggtg
     6661 ggcgtggtgg cggcgatcac cccgtggaat tcgccgctgc tgctggccgc cagcaagatc
     6721 gccccggcgc tggccgccgg ctgcacggtg gtgcacaagc catcggaaca cagttcggcc
     6781 tcggcgctgg aatttgcccg gctggtggat gaggcgggct ttcctgccgg cgtcttcaac
     6841 gtggtgacgg ggctgggccc ggaagcgggc gatgcgctgg cgcgccaccc caaagtggcc
     6901 cgcatcagct tcaccggcgg caccgaggcg gggcgggcga tcaaccagat cgccgcggcc
     6961 cgctttgccc gctgcgatct ggaactgggc ggcaaatcgc ccaacatcgt gtttgccgat
     7021 gccgatctgg atgcggcggt gaacggggtg atttccggca tcttcgccgc ttccgggcag
     7081 acctgcctgg ccggctcgcg gctgctgctg gaggagagca tccacgatgc cttcctcgac
     7141 cggctgctgg aggtggcacg cagcgccacg gtgggcgatc cgctcgaccc cggcacgtgc
     7201 gtcggcccgg tgacgaccga ggcgcagctg cacaaggtgc tggcctatat cgacatcgcc
     7261 cgcaaggaag gcgcgcgctg cgtgctgggg ggcgcgcggc tggatcggcc gggctggttt
     7321 gtggccccca ccatctttgc cgatgtgacc aacgacatgc gcatcgcccg cgaggaagtg
     7381 tttggcccgg tgctctcggt gctgcgcttc gagggcgagg cagaggcgct gcgcatcgcc
     7441 aatgactgca acctgggcct ggccgccggc gtgtggacaa gcgacatggg ccgcgccctg
     7501 cgcatgagcg agcggctgga ggcgggcacg gtctggatca acacctatcg gatgctgagc
     7561 gtgaccatgc cgtttggcgg ctacaaggac agtggcacag gccgcgagaa cggcatcgag
     7621 gcgatctgcg ccaatctgga aaccaagagc gtgttcctca accacagcgc ggcggtgggc
     7681 aatcccttcg tgatgaagct gtaggccccg cgctggcggc aggcagaagg ccccgttccg
     7741 gctggcggaa cggggccttt ctcgttcagg cgagcacttc ggcggcgatc agccggtcga
     7801 tctcggcatc ggccaggccc agccggccgg ccaggatctc acgcgtgtgt tcgcccagca
     7861 ggggggcggc aaagcgcggg cccaccgggc ccgaggcaaa gcggaacggc ggcccgttgt
     7921 agagcgcgct gcccatttcg gcatggggca gatagtgcca gtgctgccgg tgggccagtt
     7981 gcgggtcgcg ctccaccaga tcgtcggcgt tctgcaccac gccggccggc acgcccgccg
     8041 cctgcagccg cgccatcagc ccatagcctt cctgcgcgcg ggtgagggcg gccagctgcg
     8101 tgtcgatcgc gtcggcatcg gccgcgcggg caggggcggt gcgccaggcg ggccgatcca
     8161 gcagggcgcc atccagtatc tgggccagcg cctgccactg cgcatcggtg gcgatgctga
     8221 tggcgatcca gcggtcgttg ccctgcgtgg gatagacacc gcgcggggcg tggcctgcca
     8281 gccggttgcc ctgccgcccg gccacgcggc cattggcctg ataatccatc accggcagcg
     8341 gcatcagcgc cagcatcggc tcggtctgcg ccatgtcgat ctgctggccc tggccggtgc
     8401 gccatttgtg gcgcagcgcc gccatgatgg caaaggcgcc atggcagggg ttgggcacat
     8461 gatcgggata gttggtgccg gtgccggtgg gcggccggtc gggatagccg ctgaggccga
     8521 ggaagctggt gatcgccgcg atggtgatgc catagcccag catggtgcga tcggggccct
     8581 catcgccgtt catcgacatg gcggcataga tgaggccggg gttgatcgcc cgcagcgctg
     8641 catagcccac gcccagcttt tccatcgtgc cggggcggaa gttgttggcc accacatcgg
     8701 catcggccac cagccggtgc accagttcca gcccctgcgg ggttttcaga tctatggtga
     8761 tcgagcgttt ggaactgttg cgatcggcga aatagccgct gcggttcacg cccttgatgc
     8821 catccttgta gggcgcggcc aggcgcaggc tgtcgagcga gcggcggctt tccaccttga
     8881 tcacatcggc gcccatgtcg gccagcatct tggtggtgta ggagccggcg ccgatccagc
     8941 agaaatcgac gatggaaacg ccggtaagag gaagatcggt catggcagct cctcgataac
     9001 gggggcgggg gcaagggcgg gcgcggggcc tgtgcgccag ggggtggcgc tcagcttgta
     9061 gggcgcgccg ggcatgcgca ggccggcggt gggcccttgg ccgggcgcca cgaagaagcc
     9121 gcgatggctc agctgggggt tctccagcag gtccaccggg gtggacatcg gcgccatcgg
     9181 cacccgccat gcgcgcgccc tggcataaag cgcggccttg gtgtggcgcg cggcataggg
     9241 caggaacagc gcttcgaagg cggcgcaggc cgcaggggtg gtatcgtccc agtcggcagc
     9301 ggtgaagcgt tcggccccgg cgatgccttc atcggccatc cagcgggcaa aatggcccca
     9361 catggcggtc tcgccaatgc cgccggccag cagatagatt tcaccatcgc ggcagggata
     9421 gatgccggtg ccggccctgg cggcagtgcc ggcgcgctgg cggatgcggc cttccagctg
     9481 ataggtttgc gcggcatttt ccagcgccat gacgatggct tcctgaatgg agacatcgat
     9541 cagctcgccc cggccggtcc gctcggcgcg cagaagggcg gcaagggcgg ccacggcggc
     9601 aaactggtcg gccgccagca ggccctgccc gccatgggcc accactggcg gctctccggg
     9661 aaagccggcc atcgtcagca ggccgcccat cgccatcagc gtcagatcat cgccgacaaa
     9721 gccggcatag ggcccgctgc tgccataggg cgtgatgcgc agggcaacga ggcgcggatg
     9781 atcccggcac aggcccgcgc catccaccgc ggccgcctgc gggtgatcgg ccaggatcag
     9841 cagatcggcc ttgcccgcca gccggtgcag cgccgcgcgc ccgggcgctg tctccagatc
     9901 gagcacgagg cttgccttgc cggcagcgta ataggcaaag gacaggctgt tttcggggcc
     9961 cggcgcatcg ccggcaaagg gcgccaccgc ccgcatcggc gagccgcccg gcggctccac
    10021 cagcgtgaca gccgcgccga gcccggcaaa cagccggccg caataagcgc ccgacaggcc
    10081 cgacagttcg aggacgcgca ggcccgccag cgcgcgggga tctgcctgga gatcggccat
    10141 ggctcagaaa tcccgcgggc ggccatcgcg gatcagctcg cgggcgatca gccagcggtg
    10201 gatttccgag gggccttcca ccacccgcag cacgcggatc tcgcggaaca tgtattccag
    10261 cggcaattcc ttgcacaggc ccatgccgcc aaggcactgc atcgcccggt ccaccacgcg
    10321 ggtggccatt tcggtggcct gcaccttcac cgtggaagcg gcgatgcgca ccgcgctgcg
    10381 ccgctcatcg tcgcccggat tgtcgagcag ccaggcgagg cgataggtca tcatccgcac
    10441 catctcgatt tcctgatagc tgtcggcgat ccagttctgc acggtctggc gctcggccag
    10501 gggctggccg aaagtgacgc ggttgcgggc ctgctcgatc atcagatcca gcgcgcgctg
    10561 ggcatagccc aggcaccagc aggcgcattc catgcggcgg atgcccagcc ggttctgcaa
    10621 gggcgcgaag gcccagccca cttcgcccag cacttgcccg tcatccagct cgacatcgtc
    10681 gaaattgacc gcatagccgt ggaaattgcc gccgatcacg gaaaaatcct gcggcacggt
    10741 cacccccggc gtgcccttgt cgacgaggaa ggcggtcatc ccgccgcggg tgcccttgtc
    10801 cttgtcggaa acgcccagca cgatcatgaa atcggcggtg cgggcatggc tgatccacat
    10861 cttctcgcca ttcagcagcc atttgccgcc acgccgctcg gccttcaggc ggatgcccga
    10921 ggcatcggcg ccggcgctcg cctcggtcag cgccagcgcc gatttcttct cgccgcgcgc
    10981 atagggcagc agatagcgct cgatctggtc gcccttgcag cattcctgca ggaattgcag
    11041 gttggggctg tcgggcggca ggatgaaggg ggtgatcgaa tagttcatct gctcggtgac
    11101 gatgcatttg acgagggcgc ccatgccatg cccgccaaag cgttcctcca catcgatgcc
    11161 ccacaggccc agctctttcg agcgggcgtt cagccgggcc tcggcttccg gcgggatcag
    11221 cgcatcgccg ccaaggccgc gcgccgcctc gcgctcgatc accgtgcgct ccagcgggat
    11281 cagttcgtcg gcaacgaaac gcgcggtcat ctcgccgatc atgcgcgcct cgtcggatag
    11341 attgaaattc atgtcaggcc tgttcctcat cctgttcttt gcggccaata accgcgggag
    11401 caggcgcaga ggcaaacgaa aacgttgaat caggtttgag ttttacgcac aatgcagccg
    11461 cccccaccgg cgctgccgcc tgccagagaa gatgcccttg acccaatcgc gcctgccccc
    11521 gctcactgcc ctcaaaacct tcgaatgcac cgggcgcctg ctcagcttca cgctggccgc
    11581 aggcgaactg ggggtgacgc cgggcgctgt cagccggcag atcaaggtgc tggaggatta
    11641 cctcggcctg cagctgttca tccgctcggg ccgcgaggtc agcctcacca aggcgggcgg
    11701 cacctatctg gcccagctga cagaggcctt cgcccgcatc cgcgcggcca ccagcgagct
    11761 gatcggcgtg cccgaggatg cgcccctgcg ggtgtcctcc tcggtcacct tcaccatgcg
    11821 ctggctgatg ccgcggctga tgtcgttcca ttcgcgccat gccgacagca acctgcagct
    11881 gacgatgaac ctggccccgg tggatttcca gcgtgacgat ctcgatgcga cgatcaagct
    11941 gggctatgag gatacgcccc attcggtagt gcgcaggctg tttcgcgccg atctggtgcc
    12001 ggtgtgcagc ccgcggctga tcgaccagcc gggcggcccg gccgattacg actggctggg
    12061 gcgccacacc ctgctgcatt cctccgcccg gcccgacaac tggctggtgt ggctgcgctc
    12121 ggtgggccgg ccagagctga agggcgcgcg ccaactgcag ttcgaaagct ccagcctggc
    12181 ctatcaggcc gcgatcgagg gggtgggcat tgccatggcc cagctgccgc tggtgctgga
    12241 ggatctgcgc accggggcgc tggtgctgcc cttcccggtg gcggtgcgcg ataccgaggt
    12301 ctacaatctg atctggcccg atcgcacgcc gcgcaatcgt ggcttcctgc ccttccgcga
    12361 ctggatcctg gccgaggccc agcgcaccga ggccgagatc gtcgatgtgc tggcccggct
    12421 ggagagcggg cgcgccgggc cgctgtgagc agacatcaac acaccactgc aaatctcact
    12481 tgagccgcaa gcccttgcgc cgcacaaccg gcgccagaac aggcgcggcg ggaaaccagc
    12541 cgggctgggc cggccctgct gccggccaga aagggtgaga ggcatgttgc tggatgggaa
    12601 agtggcgctg gtaaccggcg ccgggcgggg attggggcgc gaacatgcgc tggcgctggc
    12661 agcggccggc gcggcggtgg tggtgaatga tctgggcggc tccaaccggg gcggcggcgc
    12721 cgatgcgggg cccgcccagg ccgtggtggc cgagatcgag gcagggggtg ggcgcgccat
    12781 cgccagcacc agcagcgtga gcgactgggc cgccgccggc gagatggtgc aggccgccat
    12841 tgccgctttc ggcactatcg acatcgtggt gaacaacgcc ggcatcaacc gcgtgggcct
    12901 gctgggggaa atctccgagg cggactggga cctgtcggcc gatgtgatcg ccaagggcac
    12961 gctggccacc atgaatgccg ccgtggccca ctggaagcag gccgggccca aggccgggcg
    13021 ggcgatcatc aacaccgcat cgcccgccgc gctgcacccg gcgccgggca ttggcgccta
    13081 ttgcgcggcg aaggcggcgg tggtggcgct cagccagtcg gccgcgctgg agctggcgcc
    13141 gctgggcgtg cgggtgaacg ccatcgcccc catggcccgc acccgcatgc tggaaggcgc
    13201 gccccaggaa ctgctggacc tgatggcccc gcgtgatggg ttcgacccca acctgcccga
    13261 gcatgtggcg cagctggtgg tctatctggc cgggcccgcc tgccgcttca ccggccgggt
    13321 gttcggcagc tggggcgatc agctgttcct tttccgcgaa tgggacgccg ctttccaggc
    13381 cggcaatggc aattgcaaat ggacgcagga acgcctggcc acggcgctgg ccgatttccc
    13441 cctgaccgac cgccgccaca tcctgatccc gggcgggcgg atggaagccg acatgccccc
    13501 cgaaccgctg ctggcgctgc tgggccaggc cgcggccgcc gtggtggagc gcacatgagc
    13561 gccatcgaga tccccaccgc cccgcgccgg ccgggcgagg gggtgcgcct gtttgccttc
    13621 acggtgggcc atgtgcacct gccgctggcc ttctttctgg agggcgaggc gggcaccatc
    13681 tgcgcgccgg tcaccgcttt tctcatcgat catcccacgg gcctggccct gttcgacacc
    13741 ggccttggcc cccgcttcgc ccgcccgccc ggccacccgc gcgaaggctt catcgatctg
    13801 gaagacgggc agacgatggc ccagtcgctg cgcaagatcg gggtggagcc ggaggatgtg
    13861 cgctggatca tcgcctcgca tttccatgcc gatcatgccg gcggcaatgc gcaattcccc
    13921 aatgcctgtg tgatcgtgca tgcggccgaa gtggcccatg cccatgccat ggccgatggc
    13981 gacctctatt gcccggccga attcgacacc ggccagccgc tgctgcgcct tgatggcgaa
    14041 catgatgtgt ttggcgatgg cacggtggtg gtgttcccca cgccgggcca cacgccgggc
    14101 caccagtcgg cgcgggtgca gaccgatggc ggcgatatcg tgctgaccgg cgattgctgc
    14161 aacatgaaac gctcgctcga cgagctgcgc ctgcccgatc attgccacga tgcggaacgc
    14221 tcgctggcct cgctgcgggt gctgcgcggc ctgcaggaca agggcgcgcg ggtgtttccc
    14281 agccatgatc cgcaattctg ggcgggcatc gccatgaatg tgccgatccg ctgaaggggc
    14341 ggtgtgctct cctgtgactt atagtcaacg gttcgctcac aaaattgagt tgaccaccgc
    14401 cgctttggct gtgaattttc ctgcaaacaa cgcgccttag gcgcaggcct acaaggcggg
    14461 atgggatgga tgggcgaatt cgattacatt gtcgtcggct ctggctcggg tggcggggtg
    14521 gtggccgcgc ggctcagcga ggtgcccggg gtctcggtgc tggtgctgga agcgggcggg
    14581 gcagactgga acccggtgtt ccacatgggc atcggcttct atcgcaaccg cccggccggg
    14641 ctgaactggg gctttgcctc agagcctgag gctcatctcg aaaaccgctc tgtcgatctg
    14701 ccgcagggcc atgtgctggg cggctcctcc tcgatcaacg gcaagatcta ttcgcgcggc
    14761 catccggtcg attacgacca gtggcgccag ctgggctgcg agggctggtc gtttgccgag
    14821 gtgctgccct atttcaagcg ggccgagggc agctggcggg gcgaggggcc ctatcatggc
    14881 ggcagggggc cgctgaaaac cgcgccggtg gatgcccgcg gcctgctgca ggaggaattg
    14941 ctggcggccg cgcaatcggt gggccatgct gttgtcgatg atctcgatgc ggcgcctttt
    15001 ggcggccttt cgcggcgcag cgatatcacc gtggggccgc gcgggcgccg ttccagcacg
    15061 gcgcgcgcct atctgcgccc ggcccgccac cgccgcaacc tcaccgtgct gacccgcgcc
    15121 cgggccaccc gcatcctctt cgaggagggc cgcgcggtgg gcattgccta tcagcgtggc
    15181 ggccaggcgc atgtggtgcg cgcgcgcaag gaagtgatcc tggccggcgg ggtgtacaac
    15241 accgcgcggc tgctgctgct ttcgggcgtg gggccggccg acgagctggc cgatatcggc
    15301 atcgccccgc tgcacgatct gcccggcgtt ggcaggaaca tcgccgatca tgccagctat
    15361 atgctgggct tcgagacgca aggccgggta agcgcgatcc gccagctgcg ctacgaccgg
    15421 gcggcctttc atttcctgcg ctggctgctg gcgggcaccg gcctgtttgc cacccagccg
    15481 ctgatgggcc agggcctgtt caaaaccatg cccggcctcg accggcccga catacaggtg
    15541 ttcttcaacc ccatccgccg cgatgtgcag ctgtggtttc ccggcatccg ggcagagcag
    15601 cagcaccggc tggaagcggg gctcatcctg ctgcatccgc acagccgggg cagcctgcaa
    15661 ctggcctcgg ccgacccgca tgaggcgccg aagatcagcc tcaacctgtt ttccgacccg
    15721 cgcgatgtgg agacaatgct gcgcggcgtg cggctggtgc gcgagatcta tgcggcctcg
    15781 ccgctgcgcg atctgctgcg cgccgaagtg gcgccgggcg agcacttgca aagcgatgcc
    15841 gagatcatcg ccgcgctgcg ccgggggctg ttcaccgtgc gccatccggt gggcagctgc
    15901 cgcatgggca cagatgcgat ggcggtggtc gatccacaac tgcgcgtgcg cggccttgcc
    15961 ggcctgcgca tcgccgatgc ctcgatcatg ccggcgatcg tcggcggcaa caccaatgcg
    16021 cccacgatca tgatcggcga aaaggcggcg gatctgatcc tgggccgccc gccactgccg
    16081 cccgcagcgc tttaggccgc gggcacacac ggacataact ggccggccat gccaccgcac
    16141 aaggcggggc aaaagggccg caaggagagg atgaatggga caaatctggc aggcgcagct
    16201 cgatcatttc aaggcgatga tcgaacagga tatcgccaag gggctgtatt atggggcggc
    16261 ggtgcgggtg gcccgcgccg gccgcctggt gttcgacgag acgctgggcc atgccgatgg
    16321 cgccggcaca caggcgctcg atcgccgctc ggtgttcagc atcttctcga tgaccaaggc
    16381 cttcaccaat gtgctgatcc tgcgcgcgat cgagcagggc cgctttgcgc tgaccacgaa
    16441 ggtgagcgag atcatccccg aatttgccgg ccccccgcgc aacgaggcca ccttcttcca
    16501 cctgctgacc cacacctcgg gctggccggc ggtgtgggaa gtggagcccg aggcctatca
    16561 gggcgatttc gacgcggtgc tggcaggcgt tatcgcgcgg gtgcacgggg tgatcgcgcc
    16621 gggccagcgc tgcgactatc agccgatggt gaaccacacg ctgatgggcg aggcgctgcg
    16681 ccgcacagac cccaggggcc gcgccctgcg cgacatctac cgcgaggatc tgttcgcgcc
    16741 gctggccatg catgacacgg cgctgggcgt gcgcgcagac ctccagccgc gccatgtggt
    16801 gcccgacatg cgcggcacgc tgccggtggc gctgcccggg cgcaccaagc cgggccgcta
    16861 tgcgatgttc gaggaggagg aggccgaaat gccccatgtc ggctgcgtgt cgacggccga
    16921 cgacatccac cgctttgccg agatgctgcg ccggggcggc gagctggagg gcacgcgcat
    16981 cctgtcgccg cgcaccatcg ccctggcccg ccgcaactgg accggcgaga tggagaacga
    17041 gctctatcgc ggccttgcca tgtcgatggg ggccaggggg tatccggcct atattggcct
    17101 tggcttctcg ctgaagggcg atgcggtggt gcccggccag tatggcacgc tgacaggggt
    17161 gggcaccttt ggcaacaatg gcgcgggcag cacgctgttc tgggtggatc cgcaagccga
    17221 tgccactttc gtctgcctca ccgccggggt gatgccggaa tgggagaaca tcaagcgttt
    17281 ccagcgctat tccgatgccg ttgcggcggc gatggtctga ggggggcgct ggccatgctg
    17341 acatatgact acaagggcaa ggtggtgctc gtttccggcg cgggatcggg catcggccgg
    17401 gcgattgcgg aggcgtttgg cgcggccggc gccagcgtgg cggtggccga tatcgcgcct
    17461 gccagcgccg aggccacggc ggccggcatt gccgcggccg gcggcaccgc gcgcgccttt
    17521 accgtggatg tggccgatga ggcggccgcg gcggcgctgg tggccgatat cgtcgcggct
    17581 ttcgggcggc tggattgtgc catcaacaat gccggcatag aggccgccaa cctgccgctg
    17641 gcggagctcg acagcgcgat ctggcggcgg gtgagcgatg tgaacctttc ggccgtcttc
    17701 tatgcgatga aggcccagat cccgcagatg ctgacccagg gcggcggggc gatcgtcaac
    17761 accgcctcga tctcgggcct gatcggcggc tataatctgg ccgcctacac cgccaccaaa
    17821 catggcgtga tcggcatgac caaggccgcc gccatggatt atgccgacac cgatttcacc
    17881 ggccagggcg gcatccgcat caacgcgttg tgcccggggc tggtggacac ccccttcatc
    17941 gcccagctgc ccccgggcct gcgcaagcgg ctgatcgatg gcattcccat gcgccgcccg
    18001 gcgcgcgcgg ccgagatcgc ccgcgcggtg ctgtggctgt gttcggccga tgccagctat
    18061 gtcaccggcc atgcgatggt ggtggatggc ggcgcctcgc tgggcggcga ggccacccgt
    18121 ttcgatgact gacccccttt cccatttgga gcatacccct atgagcgcgc ttgtgaccga
    18181 ggatctgccc cggatttccg ctaaccccac cgatgccgaa tatgccgagg cccgccgcca
    18241 tggctggctg gccaccagcg agcggggcta tgaagtgctg gcctacaagc agggctggga
    18301 ggtgatgtcg agcaaggcgt tcggcattgg cggcggcttt ggcaatatcc tcgaccagct
    18361 ggggctgacc gaggggccct accgcgacga gtggaacaag atcatcgtct gcaccgagca
    18421 tgatctgcgc gaccatatgc gcaagcccta catgcacttg ctgaaacccc agcaggtggc
    18481 caagctgcag gggctggtgc gcgagctgat ccacggcatc ctcgatgata tcaaggaccc
    18541 caccgatgtc gatttcatga aagagatcgg cgaccggctg cccacccagc tctattgcga
    18601 gctgatctcg gcgccgcggg ccatggcgcc ggaaatctat cgcatcacca attcgatcaa
    18661 cccgccgctg atcactttca acaaggcgcg gctgaaggaa tcggaagagg cctattggga
    18721 gggcatggcc ttcctgaccg agcatgtgga agccaggcgc aacaatctgg gcgatgattt
    18781 cacttccgag ctgatccgct gcgagcaggc cgggatgatg cggccggacg agacgatgtc
    18841 ggtggcgatg tcgctgctga tcgcctcgat ggacaacacc atgcaccagc tgggcctcag
    18901 cttcggcacg ctgctggagg atcgttcgcg ctgggagcag gtgctggcgc ggcccagcgc
    18961 tgccccccag gcggccgagg aaaccttccg cctgtgcccg cgcttcaacg tcatcacccg
    19021 ccatgcgccc gaggatgtgg agatggatgg ctttgtcttc ccggcgggca gctgggtgat
    19081 ggtggccacc cgctccaccg ggcgcgacga ggccgcctat gacacgcccg acagcttccg
    19141 gctgggccgg ccggcgggca aggcgctgca attcggcggc tcgtccttca gctgcctggg
    19201 ggccacgctg gcccggctgg aaatcagcga gaccatccgc atcatcgccg agcgctttcc
    19261 cggcattcgc atgattggcg actggcacaa ggcggtgggg ccgatggtga cggaatgcga
    19321 gcagctgcgg gtgtcgctgg tctgatccgg cggcggcggg cctcgcgctt cttcagccag
    19381 atcagcacgc cggtggccgc cagcaggcaa accgccgccc ccaccaggct gaccagcgcc
    19441 tgccaccagg ggccgaacac gcgggccatg tgcagcgcca cgatccagtc ggtgaagcgg
    19501 ttggcggcag gggcctggcc ggccagcgcc acgcccagca gctggccggt gtcgctgtcg
    19561 aaggcaaggc gcgaggcgcc gcctttgtcg gggatgtcgg cgctggtgga aaagccgtag
    19621 atccacgcgc cagcggccgg cgcctgccac agccagctgg ccccttgcgg gcggccctgc
    19681 gcgccggccc gggcgatcgc cgctgcggcc agtgccgcgc cgcgcgcgcg ggccgccacc
    19741 ggatccagcg cggggtgcag gcgcggggcg ggcagggcgc gcgcggccag cagggcggcg
    19801 ccatcctgcc cgcccagcgc gcgctgcacc gggccataca cccccggcag gttgaacgac
    19861 acgctggagg tggcaaacac cagcagcacc ggccacaccc acaggccgcc ggcgcggtgc
    19921 aggtcgaaat ggcgcttgtg ggccgagaca tggcgccgca ccacccaggc cggccgccag
    19981 cgggccagcc agccgacttt gcttgccagc cagctgccct tgcgcgccgc cccccggggc
    20041 gccggcggcg gcagggtgag atagaagccg aggaagcaat cacccatcca caccagcgcg
    20101 gcgatgccca tcaccagctc gcccgcatcg cccagcagca gcgaataatg caggcgatag
    20161 acaaagggca tgagattgcg ccagccttcg ccgatatcgc cccaatggcg ccagcccagc
    20221 tcggcgccgg tgtaggggtt gaggaaaagc tcgtcggccg gcggcgggtg ggcaaggccg
    20281 ggccagctca cccggatgtc gaggctgcgg ccgggcggca cgctcagcgg cagataggtg
    20341 atcgccatgc ccgggtggcg ggccagcgcc gcgcgggcca gctcggccgg atcgcgcaag
    20401 ggctggccag gatgggcagg ggcggcgcgc agtagctgcg gcgccgtcca cccctcgatc
    20461 tcctcttccc aggcaagggc catgccggtg aggcccgcca ccgccagaaa cagggccgtg
    20521 gacagccccg cccagcggtg cagcatcacc cagccctgcc ggctgatggc gcggctgatg
    20581 gcgcgcatcg cgggcctacc agtgccagcg cagcgtgccc tgcaccgtgc ggcgctggcc
    20641 ataccagcac cagcccacgc ccgagagata gcagctggtg agataggtct tgtcgaacag
    20701 gttggcgatg ttgagcgaca catccagccc ggccagccgc ggatgggtgg cgcccaggtc
    20761 ataccgcatc agtgcatcga ccagcgtgta ggcgggggtg taggtggcaa cgccatccac
    20821 agcgccggca tagacatttt cgacatggcg caggcccgcg cccagcgtca gccctttcag
    20881 ggggccaccg cgcgccgccc agtcgaggtt gagcgccaga ttgccatcgc ccgaaccgat
    20941 cagcccctgc cccacattgg ccggcgtttc ggcatcggca atggtgcgca ccttctggtg
    21001 gccataggcc gcgcgcaggg caaagccggc cggcagcggc acatggccct cgatctcgaa
    21061 gccttccgaa cgcaccttgc ccgattgggt ggaatagacc gaattgggaa tggcggtgag
    21121 gatgttcgac tgttcgatat ggaaccaggc cgcggtgacg aggatgccgg tgcccttggg
    21181 ctggtatttc aggccgaatt ccagctgctt gccgaaagag ggcttggcat tgccgatggt
    21241 gccatcggca tgcagcacgc tgccgctctg cggctcgaag gaagtggcat agctggcata
    21301 gggggcaagg cccagcccgg tggcatagag cagcccggcg cggccggtga atttggcatc
    21361 gctctggctg ctggagccgt cattggtgcg ggcccagtcg tgccggccgg acagcagcag
    21421 gcgcaggccc ccccagctca tcgtgtcctg cgcatagagg ccggtctgat actggtggta
    21481 gtcgtaggtg tagacggtgc cggcatcggc gggcgtttgc ggcacggcct gcgtgccata
    21541 ggtgggggca taggcgttga tggccgttgc cgagccgaag ccggccgcct cgaaggaatg
    21601 ggcggcctgg taatccacgc ccaccagcag gctgtggctg acagggccgg tagtgaaatg
    21661 gccgctcagc tggttgtcga aggtccagtt gtggagcgtt tcgtgggtgg catagctgcc
    21721 gcgggcaaag gtggtctggg cggggtcggt caccgtgccg gtggtgtaga tggcgccaag
    21781 gttgctgccc acatactggt agcgcccgct ggcgcgcacc gcccagccgc cgccaagatc
    21841 atgggcgagg atataggtga caccggcctg ctcgcggcgg aagaaatcgc ccggctcgcc
    21901 atcggcaaag ctggtgctga tgcgcccgtt ggggttggca aacagcgtgc cggaggccgg
    21961 cgcgccgcca taattgccgt tcttgggatc gtgcgaatag gcggccagca cggtcagcgt
    22021 ggtgcgggca tccagcttgg cggtgatggc gccgctgatc gtctggcgct cgcgcgcgcc
    22081 gaagctttgc tgggtgtggg cgccattggc gctggcataa agcctgacac cgatcgcatc
    22141 cgacagcttg ccgccgacat cggcatcgat gcgatagagg ttgtaggtgc cataagtgcc
    22201 cgccaccgag ccgtagaaat ccttgtcgag cggcagcttg ctgctcatcg ccaccagccc
    22261 gccggggctg gactggccat agagcgccga ggccgggccc ttcagcacct cgatattttc
    22321 cagccgcgac agatccacct gcggcacggc atagccggtg gcgctggaaa actgcttcag
    22381 cccatccaga tagaccggcg catcaaagcc gcgcagcttg aacaggtcgt agatctcggc
    22441 attggcgccg cgcgtttcgg gcgtgatgcc cgccacatag cgcagcgact ggttgagatt
    22501 ggcaaggccg aggttggcga tatcctgcgc cgagatggtg ctgatcgatt gcggcgtttc
    22561 ggcgatcggc aggctgctct tggtggccga ggtgacgggg gcggcgcttt ggtggcccat
    22621 caccagaatg ccggcggtgt cggcatcggc cgtatctgcg gcagtggtac tggcatcggc
    22681 ggcatcggcg gccagcgccg gatgggcgca cagggcaagg agtgagatgc cggcaagcgg
    22741 cagggcatag gcgcgcacga aagggtccct catgagaaag gcgcccggcg gggcgcaagc
    22801 ggcggagtgg gggccggggc cggccgatgg tcaggcggcc agccccgggg gcaggagcga
    22861 cgtggccggt tggtccggcc ctggccattc tgctaatgcg aagagttctc aattgcaagg
    22921 ggaaaacgtc ggccccctgt ctgcgggctg acgctgcatg ccggcgcccg gtggaacggg
    22981 gctgattccc tgttgcgttg cggccggttt tcgccaatca ttgcatatgc aattatatcc
    23041 gatcgcgccg ccgcaaccgg ggcagatggg ctttgccggg ggttttggcc aatgccgcac
    23101 tacgattgcg tgattatcgg ttcggggatc aacgggttgg tgtgcgcggc catgctgggc
    23161 aaaaagggcc tgcgcgtggc cgtgctggaa cgggccgatg tggccggcgg atgcatccgc
    23221 accggcgaga tcaccctgcc gggctatgtg catgacatgc tctcgggctt tcatccgctg
    23281 ttcgtcagct cgccggccta tgcggcgctg ggggcggatc tgcatgcgcg cgggcttgcc
    23341 tatagcaatg cggcgctgcc caccggcgtg ctgctgcccg atggccgctg gacggtgatg
    23401 gcgcgcgaca gggctgccaa tatcgcccgc ttcgaggcgc tggcccccgg cgatggcgcc
    23461 gccttcgacc gggcgatgca ggcggtggcg gccgatgccc cgctcacctt cgcgctgctg
    23521 ggcgaggatc tgtggcgccc ggcaatggcg cgcaagctgg cgggcgcggc ctggcgcaag
    23581 ggggcgggcg ggatgatcgc ctatgcgggc gcggcccttg ccagcgcgcg cgactggctg
    23641 gaggcggatt tcgcgtcaga cgagctgcgc gccctgttcg caccctgggt gctgcacacc
    23701 gggctggggc cggatcaggc ggtgtcgggc tatatggccc aggtgatcgc ctttgcgctg
    23761 gaggcggccg gcatgccggt ggtggtgggc ggcagcgccc ggctggtgga agctttcgtg
    23821 gcgctgatcg agacgcaggg cggcgcggtg ctgtgcggcg ccgaggctgc cggcatccgc
    23881 gtggcgcagg gccgcgcgca aggggtggat ctggccgacg ggcgccattt tgcggcgagc
    23941 aagggcgtgt tcgcctcggt cacgccgcaa gcgctgtatg gccggctgct gccgcccgag
    24001 gcggtgcccg tggccgtggc gagggcagcg caggccttcc gctatggccg ggccgatatg
    24061 cagatccacc tcgcgctcga tcggccggtg gcctggccgg cggcggaact ggcagacacg
    24121 gccatggtcc acctgacggg cgggatagac gatgtggcgc aggccgtggc gcaggccaat
    24181 gccgggctgc tgcccgcccg gcccacagtg gtggtgggcc agcccaccgc gcaggatccc
    24241 tcgcgcgtgc cacagggcaa ggggctgctg tggctgcaat tgcaggaatt gccgcgcgtg
    24301 cccaggggcg atgcgctggg cctgcttgat ggcacgcagg gctgggcgcc gggcttgcgg
    24361 gacgcctatg ccgatcgggt gatggcccat gtggagcggt ttctgcccgg ccttggcgcc
    24421 agtgtgctgg cccgccaggt gctgggcccg gccgagctgg aagcgctcaa ctgcaatctg
    24481 gtggggggcg atccctatgc cggcgattgc gcgctggatc agtccttcct gtggcgcccg
    24541 ggcgcggcga tgcgcaacca ccacacctgc gtgaaggggc tttatcacat cggcgcctcc
    24601 acccatcccg ggccggggct ggcgggcact tcggggttta tcgcggccgg ctatctgtga
    24661 gccggccggt tcaggcaaac agggcgggga tgggggcaaa cagcccgtct ggccggcgtt
    24721 cgggtgcggg ccaggcggcc ttgctgtggg ccagcttcag gtcgatcagc gtttcgcagg
    24781 ccttgtaggc ggccaggcca gggttgatca ccggcaccgg caggcaggcg gccagatagg
    24841 cgtggctctg gtgcatggtg gtggagccca gcacgatcac atcggcgcca tcctcggcga
    24901 tggcgcggcg cgcttccgcc tccagccggg cgaagacgat ctcctccttg ccggccagca
    24961 gctcatgcgt atccgggcgg gtggcgatat ggcgcacgct ggccagccgg tcggccaggc
    25021 cgtgtttggc ggcggtttcg cgggccagct gctgccaggg ctgccacatg gtgaggatgg
    25081 aaaagcgccg ccccagttgc gccgccagcg acagcgccgc ctgccccgcc cccaccaccg
    25141 ggatggcgag gcacgaacgc agccccgcca gccctgaatc gctgagggtg aagctgcaca
    25201 ccgcatcgca gccggccgct tctgcatcca gccccgcatc cagcacgaag gcatcggcaa
    25261 ggctccattc gcaggggctg tcgagcaggc tggcgcccgc ccggcagccg cgaaattcca
    25321 gccgcatatc ggggcgcagc agatgctgcg gaatttgcgc ggcaaaatgg gccagcgcgg
    25381 catcgggcag cggcaccggc aggatcatgc ggataagcgc catggggcag ggccttttct
    25441 ggcgggaggg aaaagggcgg gggccatccg gcgccccgcc ccaaggtgtt agtaagtgta
    25501 gcgcagcgac acgttccacc agcgcggcat gccatagctt tcctcgttgc agccgcacag
    25561 cgtggcgagg tcgtaggtca ctgtcttgta gcgctcgtcg aacatgttct ccacgccgat
    25621 gcccagccgc agcggcccgc ggttccagta gacatgcatg ttggcgagga aataggggtt
    25681 gatgatatcc gcatcgaaat tgcggatgtt ggcatagacc ttcgagcgcc agctgccatc
    25741 gatgttgaag ttgatgtcgc cgccggccag ctgctcgggc agggtatagc cgatctgcgc
    25801 ggccagggtc ttggcgggcg tgaaggtggg gcgcacatcc ttatagatgc cggtggcaat
    25861 ggccacatgc ttcaccttgg catcgatcag cgagaggcca aggccgatgt tcagcccccg
    25921 ggccggccgc aggttgagcg aggcctccat gccgaagttg cgcgcatcat tgttgctgac
    25981 gatacccgac acgttggaga aggtgaaggc ctggtaattg tggtaatcat agtaatagcc
    26041 cgacaggtcc agcgtcagca tgttgtgcca gaagccggtc ttgaggccgg cctcataggc
    26101 caccagcgtt tcgggattat aggcaatgcg gctgggcgag agcgaggccg agccatcggg
    26161 cagctgggcg ttgtaggcgc cgcccttcac gccccggttc acgccgaaat agagcagcgt
    26221 gcccttggcc gggcgatatt ccacctgcgc cttgcccgcc cacatcagcg tgttgcgctt
    26281 gttggcatag ggatcctgca gctggaacag ggccgggcca taggtgaatt cataatcatt
    26341 ggtgttggca tgggccatgg aatcgaaggt gtaatcctgg tgctcgtgga tgatgcgccc
    26401 gcccaggatc agcgtcagct tcggggcgaa atcatattcg ccctgggcaa aggccgagat
    26461 cgattccgtc ttcaggttga catcgttgat gagatcgatg ccgctttgcg catagccggg
    26521 ataataggcg gcaaacactg aattgctggg cgccttgaag ccattgtgca ccagcgagtt
    26581 gatatcgagg aaataggcgc cggccaccca gcgcaccggc ccctttttgg tgccggcgat
    26641 gcgcagttcc tgcgagaagg aatgttccac gctgtcgctg atgaaatcga tgaggttttc
    26701 cggccccgca tcgacatcgg tgccataatc cttgcggaag tggcgataat cggaaatcgc
    26761 cacgatctcg gcgccatcaa gctggtagtt gatgtggacg gccgtgtccc aggcgaagag
    26821 gcggttgcga tcggggatcg accagtcgga ggagacttcc aggctctgct tgccgggcgc
    26881 gttgtagcca taggaatcgc cattggcgaa caccacattc accactcggc cctggctgtc
    26941 gtaaaccgcg cgggtgccgg tggaattata ggggttcacg ctgaggtgct gggccagata
    27001 ggagccggtg aggcgcacgg tcagccggtc ggtcggctcg aactgcaatt gcgcgcgcac
    27061 cgccttgctg ttctcgccgc ccagatccgc gcctgcgccc ccttgcggcg cggtgctgcc
    27121 ggccgtgagg ctgctggtgc cagcgggata gaggttcttc agatagccgg tgttgcggtt
    27181 ataaaggccc gaaacgcgaa actgcaccgt atccgacagc ggcaggccga tcgccgcctc
    27241 cacccgcaca tggttatagg cgccataggt cacctcggca taggcattgg gcttgtcgga
    27301 tggcttcacc gggatgaaat gcaccaggcc gccggtggca ttgcggccga acagcgtgcc
    27361 ttgcgggcct ttcagcactt ccacgcgctg cagatcgaag gtgccgaaga tctggccgtt
    27421 gagattggga atataggttt catcgacata gaccgcgacg ggcgcttcga tggcatcgct
    27481 gaaatcattc tgggtgacgc cgcggatgga gaattgcgaa tcctgcccgc cgccgctggc
    27541 cgaaatcgac acgcccggcg tgagacgggc gatatcggtg gaggattgca cgcccatctt
    27601 cttcagcgtg tcgccggaaa aggcgctgat ggcgatgccc acatcctgca ggttctgctc
    27661 gcgccgctgg gcggtaacga cgatgtcgcc cgagccgttg tcggtgctgg cagcgggcgt
    27721 gtcggccagc gccagctgcg gcgccagcgc cagcgccgat gctgtgaaca gccagactct
    27781 cttggtgtgt ctcatggtgg aactccccag tttcttattg gttgcaaagc cggggtgcgg
    27841 cgatttattt tatgttcaaa atattattgc gcttgcgcac ccctgaacga tcccctcatc
    27901 aacctgtgtg attatgggcg cggctacgcg ctgttatgtg ccctgaatgg caggccgagt
    27961 ggcagggggc cccgtgaaag cggccggccc tgcccccccc tcaaccgacg agcgcgggcg
    28021 ccctggcgct gctggtgtcg ctgatgcgca ggccccgcgc ttccagcagg ggcagcacat
    28081 tgtcgcggaa gaacggaaat tcggcgacat aatcgacgaa cgacaaggtg gtgccggcaa
    28141 agcccgtttc atgcagggcg cagatgccat cggccacctg ctcgggcgtg ccgatcagcg
    28201 gatagccgcc atggccggcg gcaaagcggt ccttgatcag cgtcagcaga tcatgcggga
    28261 aggaatgcgc atgggcaaac tgcagcgcga tcacttcctc cagcgtgccg acatcgcggt
    28321 aatcatgggc atagtgctgg agataggcct tggcctcggc ctctgtcggg cggcacacca
    28381 catggctgaa ggtgagcact tccacctcgc ggccattggc ctgcgccgcg cccttcagct
    28441 gctctatctc gggggcagag cgggcgaggt cgatcgccgg ggtgaacagg taatcggcat
    28501 tgcgcgtggc aaaggcgcgc ccctgggcag agccggccgc attgatgatc ggcggcaggc
    28561 cgttgaacgg cagcggatcg ctccatacgc cgcgcaggcg gaagaagctg ccgtcgaaat
    28621 cgaagggttc ggtgctggtc cacagcttct tcagcacatc gaaccattcc tgcgcatggg
    28681 catagcggct ctcgtggtcg tcgggcagat tgaggcccag cgcgtcgaat tccggcttgt
    28741 tccagcccgc cacgatgttg agcccggcgc ggccatggcc gatctggtcg agcgtgacga
    28801 tctgcttggc ggccaccacc gggttgaagg cggccgcatg cacggtggcg atcaccgcga
    28861 tgttgcgggt ggacgccagc aggccggcgg cccaggtcat ggtttccagc acgccgctct
    28921 ggaagttcga cggaccgccg cgatagccga tccagcgcgc caccggcagc atgaaatcca
    28981 gcccggcctc atccaccatg cgcgccagcg ccaggttctc gtcccagctg gccttccagc
    29041 gctcgggcac attggtgatc gacatgccgc ccgaacagtt ggacgaaaat gttccaagct
    29101 taaaatgttt gctgcgcatg cgattggcgg tctgcgtcat ggaacagctc tttccgtaag
    29161 aaggggggct cgcgccagcc cttgggccgg gcggcgcaag caacaacccc agcaaacccc
    29221 ttcacgcccc cgatggctat cctttgagcg caatgcctag cccggattcg ccacaaactg
    29281 cgcgccctgc ggtgtcgggc cgggcttggc gttcccgggc tagcgcgggg gcgcggccgg
    29341 cggcaggttt tgcccatgcc cggcgcctgc cgctggcccg ccgggccgcc catccttctg
    29401 acataaggca gcaaatccca tggcagagac gattctcatc ccccgcaccg gcgctgtggc
    29461 cgctgatgat ccggcctgtt tcaaccgcct gcactgggcg attgccccgg tggcacgggc
    29521 caggcccggc gacaccatca tctacgaaac gcgcgatgcc ttcgaccacc agttcacccc
    29581 cacctcgccc catgcggcgg cggccacggt ggatggccgg cgcgtgcatc cgatgaccgg
    29641 ccctgtgcat atcgaaggcg tcgagcgggg cgatgcgctg gcggtgacga tcgtcgatat
    29701 cgagcccgac agctatggct acaccagcgt ggtgcccggc ttcggtttcc tgcgcgatgt
    29761 gatccccggc ccgttcctcg ccaactggaa gctggatcgg ctgtcggcca catcggagca
    29821 gatgccgggc atccgcgtgc cgatggcggc ctttcccggc tccatcggcg tgctgcccgg
    29881 cgtgccggaa accgccgatg cgctggcccg cgagggcgcg ctggccgaag ccggcggctt
    29941 tgctcccggc ccggagccgg ccgaagcggt gcccgccagc ctgttcggcc ccggcgcgcc
    30001 ctatgagaag gaaggcctgc gcacctttgg cccgcgcgaa tgcggcggca acatggatgt
    30061 gaaggcgatg caggtgggca ccaccatcct cttccccgtg caggtggatg gcgccggcct
    30121 gtggacgggc gacatccact atgcccaggg cgatggcgag gtgtgcggca ccgccatcga
    30181 gatggacgcg cgcgtgaccc tgcgcgccga ggtgatcaag ggcggcggca ggaacatgcg
    30241 ctttccccac atcatcggcg gcaaccagct ggcgcgggtg gcgccggggc ggttctatgc
    30301 ggttaccggc ctgccggtga agaaggccgg cgaactgccg ccgcatctgg cctggctggg
    30361 cagcgccaag ctcgccgcgc tcaccaatgt gtcggaagac ctgacgctgg cgacgcgcga
    30421 cgcgctgatc cgcatcatcg actggatggt ggagaacaag ggcctcagcc gcgagcaggc
    30481 ctatgtgctg gcctcggtgg cggtggatct gcgcattggc cagctggtcg atgtgcccaa
    30541 tttcgtcgtg acggcggtgc tgccgctcga cgtcttcgaa gattgaggaa aaggaggcac
    30601 cccatgaacc tgcagcatcg ctgggcagag gcaaacccct ggctgggcac cggcccgatc
    30661 ccggtcgagc cctatatctc gccggcctat tacgagaagg aaaaggagaa gatcttcaag
    30721 cgcgtgtggc tgtgcgtggc gcgcgaggcc gaagtgccac agcccggcga ctacaaggtg
    30781 aagcgcattg ccgcgggcga cacatcggcg atcatcgtgc gcggcaagga tggcaaggtc
    30841 cgcgccttcc acaacatgtg ctcgcaccgc ggcaacacgg tggtgaccga aaccggcgag
    30901 gaaacctatg gcgccagccg cgccggcgtg atgacctgcc gcttccacgg ctgggtctat
    30961 ggcgccgatg gcgcgctgaa gcaggtgccg caggagggcc gcttctttgc ctgtttcgac
    31021 aaggccgaca atggcctggc ccccatccat tgcgacatct ggcagggctt tgtcttcatc
    31081 aacctggcag cacagcccga ggaaagcctg atgagcttcc ttggtggcta tgccgatcat
    31141 gtggcgggct atgatttcag cgcgctcacc tatcactacc attaccacac catgctcaac
    31201 tgcaactgga aggtggcgca tgatgccttt gccgaggcct atcatgtcga cacgatccac
    31261 gccggctcct tccccaacac cttttcctcc ggcatccaga acgtgaagct gatggggccg
    31321 caccgcacct gcgccgtgtg ccttacgctg ggcgccaacc ccacgccgat cggcacgctg
    31381 gcggccgaga aggcgcgcgg atcgctgacg ctgcgggccg ccgaatcgcg cctgccgccc
    31441 accatcaacc ccgacaggcg cagcgatttc gccttcgagc tcagcgttct gttccccaac
    31501 acgctggtcc atgtgtcgga tggcatctgg ttcacccacc agttctggcc ggtggcccac
    31561 aaccagaccc tgtgggaagg gcgctattac gtgcagacgc cccagaccca cagcgcgcgc
    31621 tgggcactgg aacaggcgat catcctgcag cgcaacgcct ggctggaaga taccgctacc
    31681 atggaagaca ctcaccgcgc catgcaatcg ggcgccaagc aggtgcagtt cctgcaggat
    31741 gacgagatcc tcatccgcca cggcgcggcc gtcgtcgatc aatatgtgca ggcctgagga
    31801 gagagccatg agcgagatca tcctgccccc gggcttcgag gcgctggccg tgcatgccga
    31861 atgggcgctg gacacgcccg atgaacgcca ccgcgcccgc cttgccagca cgcccgcccg
    31921 gctggaggcc ttctacaacg gcattctgcc ctggctggaa aagatcctgg aagagctgga
    31981 ccgcttcccg ttgggggcgc tgcccgaaag ccaccatgcg ctgttcaacc tggcctttgc
    32041 cgtcatcgag gtggcgccgc atgtggagct gtatcgctcc agcccgggcg tgccctttgc
    32101 cttcgaggaa ctgcgctttg tggcggcgca tggcgggcag cccacatggg ccggccggcc
    32161 gcctgcggag atgcgccgat gaacgaggcg cggatggcgg cgatggagca gcggctggcc
    32221 cggctggaag cggtggaggc gatctgcgcg gtggtggcgc gctatgccat cgcggcggat
    32281 ctccacaatg atccggcgct gtttggcgcg ctgtttgcgc aaagggcggt gtgggaagcg
    32341 cgcggctttg gccggcacga ggggcgcgcg gcaattctgg ccgcgctcgc cgaagtgggc
    32401 cggcagacca tgctgtggac cacccatttc aacgtggcgc cgctggtgga gccggccgca
    32461 gacctcgcct cggcccgctg ccgctggtat ctgtgggaac tggcgcgcat ggccgatggc
    32521 gccgatggcg ctgatggcgc ggccgatcac tggatcgccg gcacctatga cgcccggctg
    32581 atcgtggaag ggggtgcctg gcgcatcgcc gaactgcagc tggacctgcg gctgatgaca
    32641 ccagtggcag cgccggcctt tgccccgcag gtggcggcat gagcgacctg cgcatcgcgc
    32701 ttgccacgct ggatgccgat ggcattacct gcatcgccgg gccggccggg gccgcgctgg
    32761 tggtgtgcct ggtggaaggc gcctattacg tgaccgacga cctgtgcagc catggcgatg
    32821 cctcgctggc ggaaggctgg ctggaggggt tcgatcttgt ctgcccctat cattccggcg
    32881 ctttcgatgt gcgcacgggc gcggccagcc gggcgccctg cgcgctgccg gtgcgcagct
    32941 atgccgtgcg gcgcgagggc gaggaactgg tgatcggccc cgcgcaaggg tgaacggaac
    33001 ggccctattt gctggccccg cgcatcagcg tttcggaagg ggtttcgcca aagcgctcgc
    33061 gatagtcgcg ggcgaatttg ctgagatggg tgaagcccgt ttccagcgcg atctgggtga
    33121 cattgccgcg cccggcgcgc agggcccggt gggcgctgtc cagccgcagc ttcttcagat
    33181 agcccagcgg cgtatcctgc cggtggcggc gaaaaccggc ctgcagcgag cgcgaactga
    33241 tgccggcggc ctggatgatg gcatccagcg tgaccggttc gcgcaggttg gcgcgcatga
    33301 aatcttcggc ccggcggatg tagtagggga tggccgggcc cgctgccggc tggtgatggt
    33361 cgtagtaatt gttgggcagg ctgagcagca gcaggcgcat cagcatctgc tcggtggtat
    33421 cgatcatgcg tgaatgggtg aggccgcgct ctggcccctg catctcgctg cacagcaggc
    33481 tgacgaaatt gcccagcgcc gcggcctggc ccaccagcgg cacggcatgg ggggtgaatt
    33541 gcagcggcgg gcaattgcgg ccctcgtcct gggcgatcag ctgggtgagg gcggtggtgg
    33601 ggatcttgat ggtgaagtgg cgataatcgg cgttgaagcg caccttggcg ccaaggctga
    33661 gatccagcgc gcacatctcg cccgggcgca gcagcacttc ggcgccggcg tggcggatct
    33721 cggcggtgcc ggccagcgag aactggatca ggtggatatt gtcgcaaggg gcgatatcgc
    33781 accacacctc gccgtcgcgc ccgtcgtaga tggtggtgtt gaagctgagc tggcgcagcg
    33841 gcgtttcggt atgggaaaag ccgaagccct tgggctctgc ccgcacctgc aggctgtggc
    33901 ggcagaacac ctggctcata tgcaggcgca catgatcggg atcatgggtg cgaaaggcgc
    33961 gccccggatc ctgatagagg ccttgcaggg cgccggccag cgcatcgcgg tgcggcgcag
    34021 ctgccatggc gccatcggct tgagcaatca tgatgcgtcc cccgaaacgg tcatttcgag
    34081 ttcgcccctg cggatatatt ttatgctgca aataatatcg gatgcggggc ggcctgtcca
    34141 gccccgccat gcatgcgcgc gcggcgatta ttcgggctgg cctttggcgg gtgatttgtt
    34201 ttaatgatga aatagatcgg gcggcaagag gacgaggatg gctttgacaa acccggacgc
    34261 agcgcagccg gccgaggccg cgcccgccag cgaggaccat aaggctggca gcctggattt
    34321 gcgcctgtgg atccgcctgc tgggcagcgc caagctgatc gagaagcgcc tgcgccgcaa
    34381 cctgcaggat cagtttggca ctaccctgcc gcgtttcgat gtgctggcca cgctggagcg
    34441 caccggccag ggcatgacga tgggcgccct ttcgcgcgcg ctgctggttt ccaatggcaa
    34501 tgtcacctcg atcgtgcggc agctgcatga aagcggcatg gtcgccgtgg tggctgatcc
    34561 ggatgaccgg cgctcggcga ttgtcagcct cacgccgcat gggcgggcgc aatttgccat
    34621 ctatgcccgc gcccatcgcg gctgggtgca ggaggcactg gccggctttg cgcccgcccg
    34681 gcagcgccag ctgttcgagc tgctgggggc cctgcgcgcc accttgcgcg cgcaggacgg
    34741 ggactagcgc ccctcaggcc gggcagctgg ccagctgggc gctgcgggcc tctgccagcg
    34801 cggcggcggt gggcgcgggc atcgccatca ccgcctcgaa ctgggccagc gtgcgctcga
    34861 acgtgtcgcc atagcccttg atcagccgct ggcagcgcgc ggtttcggtg gccagcgcca
    34921 gatcgtgccg ggcctggctg gccatgcgcg ccagccacac ctcgatgcgc gcctgttcct
    34981 cgccatagcg cagtgtgcgc cgccgccagc gccgccccgc tgccaccagc cgcagcgcca
    35041 gaaaccagcg caggctggtg gtttgcgcca gccgcccgcc ggccagctgc cgctccagcc
    35101 agcggctgag ggcagggctg gccagcagcc tgcgggcaag gccggcgggc agcgtttcgg
    35161 ccacttcgcg caggcgcgga tggaagaatt cggtgagggt gaggatctgg ccggattgcg
    35221 cgcgcacttc ggcccgcacc cgttcaaggc ggctggcgcg gatcttcaga tcggccacgc
    35281 ggatggtgtc ctcataggcc atccacaggg cgaggtggcg ggcggtctcg cgggtcagct
    35341 caaagggcgc ggcatcgagc gcggcgatgg ccgccagccg ctcgagataa agcgcggcat
    35401 aggcggcatc ctgatagtcc atcagccggg ccaggccatg caggacaaag ggcagggcaa
    35461 aaggcagggc ggcggcaggc aggctttgcg ccgcgcgttc ggccaaggcc cggccggcct
    35521 cggtggtggg ttgggggggc gggctgtcgg cctctgcggg cagcgccagc gggccgcccg
    35581 cgccggcaaa gcctgcctca aagcccgcca gattgctggg cacggccttg ccgctggcgc
    35641 ggatcgccgc ctcgaacgcc gcgcggggga agggcagggc gccgctgccg gccagcgcgc
    35701 caaacatcac cgcgctgatt acgctgccgc tggcgatggt ggccgctgcc atgtcgaagc
    35761 cgatgaagcg ggcggtgcgc tcgctggcgg cggcgaggat tttggcgctg ttgccgcgcc
    35821 cgtcgcccgc cacctgcttt tccgagatgg catagatgcg gtgggtggag ccgatcagcg
    35881 tggtgcgctt gtcggcaaaa ccgcgcagca gggcgcggcc cgcttccatc agttccgagg
    35941 cgatcaccac atcgacatcg cccggcatcg gcatttgcgc catcaccggc gcgcggccat
    36001 cggcgccggg gcgggccagc tcgacataat agaccgtgga gccggtgcgc tgggccacgc
    36061 ccggcaccga tgtgccctgc gcgatccagc ctgccgcgcg ggccatatgc tggatccatt
    36121 ccgccagcac gccgccgccc tggccgccca gcgcaagaat ggcgatggcg atgcgtttgc
    36181 tctcgttcat gggcggaaat ccttcagaag gcacgggcaa ggcgggcctg ttcatcgcgc
    36241 gcctgcagcc agccggtcag ccgctggcgc cagcgggcgc gcagcttctc ccagcgggcc
    36301 ggatgggtga cgatgcgcgc gcggaaaaaa ctggggcaca gcacggcggc atgggcattc
    36361 tcgccgcaca ggccgcagcc cacgcagctg tcgtccacat ggctcaccgg gtcctgg
//
LOCUS       DASRYE010000020        29192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_195380,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000020 DASRYE010000000
VERSION     DASRYE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 29192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29192
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195380"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1250
                     /locus_tag="VFF98_01080"
     CDS             120..1250
                     /locus_tag="VFF98_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62247.1"
                     /translation="MTATASQPTPPPPGPPEEIWFTRCPVPTATGIAYKLGWLSEAFA
                     ADGIALRTLQESGSELARHHYDHRLPTLIREGGNMLAFGARAQGEPTRLIGLTWIEES
                     QLILTRPGTGIARAEDLKGKRLALPGWNRNPIPEHVRGTSIARGMSLAGYKGALASVG
                     LTLDDVTLVETPDATRELAGSGRDIDGEDRRLQRGLGRLWPLQALVDGQVDAIYVKGA
                     SALDAARAAGLDVAVDLDAIPDRRFRVNNGTPRPITVHQSLIDDHFDYLVRFLAITLR
                     AADWVARQPDQLRGILAFETAASPEAVNHAYARLHEGLRPTLDADRLALFDQQKTFLF
                     THGFLDADFPLADWIDPRPLEAAIALNANQPQPLREPAPLPA"
     gene            1286..2026
                     /locus_tag="VFF98_01085"
     CDS             1286..2026
                     /locus_tag="VFF98_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZU62248.1"
                     /translation="MTHGLSKETIAFVRETEADARKAFRWLRETQTTSPSATVGFAVR
                     VPGEDKIVTLAYAGLWAEDPDAPITEVIDFDGNTYWGGKPSGESRYLKIFRKYEDFQA
                     ISHVHAPNLGAYSQAHSELPLLYVPNRRFRTTTHLPVYINRRQSEADFILEAIAADKQ
                     TPGIVEANGGATVWSFKGLRHLVQNIILLEEGARFQVLAAALGGSRPFGPGVLEQQWK
                     IGGLVPADAHVTDDGQILLANPAPQAAE"
     gene            2085..3431
                     /locus_tag="VFF98_01090"
     CDS             2085..3431
                     /locus_tag="VFF98_01090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU62249.1"
                     /translation="MTSPKRQLALAVFLTRHGHHPGAWRQPGSAPTGLPDFAHWARLV
                     QTAERGKLDAAFFADFAGMAGDNTRGIARRHAFLDFEPLTAISALASITRHIGLIATV
                     NTNFEQPYALARRLASADHISGGRVGWNIVSSLDPGAAKSFGITQPATHAERYARADE
                     FVALAKALWDSWEDDAFEGADKASGIYLQADKVHPVHYRGAYYQADAVLDVPRSPQGY
                     PAFAQAGNSEVGRDFAARHANMIYAAAQFIEDAQDFYGDVKRRVVAHGRHPDELKVMP
                     GLSFTLGSSRAEAQDRFAALEEAVDFSGDLNLMGQDVSAYPLDGPLPDLPEPENGKGR
                     WRQLTALARRENLTIRQLFLRFNAVRGHRVLLGTAADIADGMEEWFTAGAADGFNLIP
                     PVLPTGLDAFVDQVVPELQRRGLFRTEYEGRTLRDHLGLARPANPHTARQTVTEPA"
     gene            3454..4536
                     /locus_tag="VFF98_01095"
     CDS             3454..4536
                     /locus_tag="VFF98_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62250.1"
                     /translation="MTQNHLDTLWYTRCPVPTGLGIAIQQGWLGEAFAQRGTQVKSLL
                     ESPDFAVRESHFSHTLANSVRHGGSIPAISAFARGRDTRLIGLSWADETQLILARPES
                     GITTVRELKGRRFGLPNWKQVEIDFSRAQAIRGLENALRLEGLEVADVELVNQDIDSH
                     YSEGAARSIAGTVNWTGRTPSRARANNLEVIALLRGEVDAIFLKGANGAQVAHQFGLT
                     TLIDTGAHPDPLVRSNNGTPRTLAVDGQLIEQHFEAATTIAEQVLRAEAWAHRHPEEV
                     RRYLGREANASEFWVEAAYGADAHLKLNTDLAEQSVAALQDFTQFLHRWGFIPRSFAV
                     ADWIDPRPLAEARSRLQQAAPLAAAE"
     gene            4540..6360
                     /locus_tag="VFF98_01100"
     CDS             4540..6360
                     /locus_tag="VFF98_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62251.1"
                     /translation="MGKPLAVPGAQPAPRQAGAGGHPFLRCLAIYAEMPRRFLLTALL
                     LGLVNAAMLGQQVLVGRAVQEAGRGQLVARLPGGGLDYHRAGWFAAVLLGLAALRGGL
                     QLGAGLLSLSIGQELLTRLRLRILAQIQRLDLAYHWRHGVGEMVTRMTRDADKLRDGL
                     INFWRQMFETGLVVVLSLGLLAGWSVWLALVPGGLFVLGIWLLARETGTLVALDRRIG
                     DAYDAVTQDLSEGVNGVRVIKAFALGQARARRFAAFIDLFRQEARAALAYASRRVPLP
                     QAIIALGHAWVLGCGAVLVARGSLGLGALVAALLVVNNLVLRVENIGRLLQVVADASA
                     SAARIWDVLDARPAILGGPAPVPAGPLGLRLEQVSVAPLGDGGRVLHDVSLALAPGEV
                     VALVGPTGAGKSILASLLPRLVEVDEGRVLVGSDAAGWQDVRGLDLAELRRRVHVLPQ
                     ESFLFSDTLAANLRLSAPAAEDATLIAALEQAAAGDVLPRLAEGLETRLGDRGVTLSG
                     GQRQRICLARALLSGADILVLDDATSALDAATEQRALANIRALRGSGAAPTVLIVSSR
                     LSTILAADRVVMLAAGRIVAQGPHAQLAAGNAAYRALMGI"
     gene            6362..8185
                     /locus_tag="VFF98_01105"
     CDS             6362..8185
                     /locus_tag="VFF98_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62252.1"
                     /translation="MNGFRGDSALSEIELEERLAQKRLGRGVGGRLWPLVAPVRRLIA
                     AVVAIELAQVAVIFARPALIALVIDRGLRHGLQPALVGWACAGLAACWAARFGLGGLS
                     QYAAGLAAIRVLNDLRARVFAHVQTLSVGYFDRTRAGRIIARADRDVDTLEPLLIQGP
                     PELLSAVLRLVVAGGLLWQVAPALLLGLAGVVPLLLGATALFKRISQASWAQVAECRS
                     RFTAHLVESVAGVRLIRQAGRAAENLAAYRRLLDAFNTALIRGNLRSGWFAPFTGLLT
                     TAGVVLVLVIGGRGVALGTLSLGQLTQSLFYVYLFLAPLQEFSDLFERLANGTACAQR
                     IFLLLDTAPDIADAADARDLPRLAGAIRFAGVGFGYVPGRRVLQGLDLAIAPGEVVAI
                     VGPTGHGKSTLVQLLTRFYDVDEGAILLDGHDIRAISEASLRRQVGVVLQDNVLFGGS
                     VLDNLRLARPAASDAELIAAARELGADAVLEALPHGYHTQVGALGGQLSQGQRQLVCL
                     VRAYLADPAVLVLDEATSAVDVATEQRIQRALRRITAGRTSLIIAHRLATIREADRIA
                     VIREGAIVELGPHDALLARGGHYAALYAAYAAPPAPLPAMA"
     gene            8244..9002
                     /locus_tag="VFF98_01110"
     CDS             8244..9002
                     /locus_tag="VFF98_01110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62253.1"
                     /translation="MTDLFDLTGQCALVTGSTRGIGLAIAKALIAAGAKVVISSEDAA
                     DTARVAAELAMPGIACDMREEAALEPLVAGTVAALGGLDILVCNAGITGRPGPFASLD
                     MADYARVMAINLTAQVLLCNHALPHIAARGGGSAILVASLAGLRGNGAINAYALAKAG
                     VAQLARNLAVEWGPRAVRVNALSPGFIATELSKPLLENEAFMARRMGMTPLRRPGRPE
                     EIAGAAVFLASAAGGFVTGHNLVVDGGTLITDGS"
     gene            complement(9007..9321)
                     /locus_tag="VFF98_01115"
     CDS             complement(9007..9321)
                     /locus_tag="VFF98_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HZU62254.1"
                     /translation="MERIAFRMRLKPGMLAEYRRRHDEIWPELAMALKAAGIHDYRIF
                     HDADTDLLFATLLREEGHGMDALPDLPVMRRWWAAMAPLMEVAADDSPRSWPLTEVFL
                     LP"
     gene            complement(9337..10824)
                     /locus_tag="VFF98_01120"
     CDS             complement(9337..10824)
                     /locus_tag="VFF98_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62255.1"
                     /translation="MAPPLSAARPVRLVNYLAYGANDALGAGSMAIVSGWLLLFYTRF
                     CGLSAGQAALIFAAARLIDAVLSPLIGHLSDHMGAHGLGRRFGRRRVFILAAIPLLPS
                     FALMWLPGQGFWYYLVSYVAFEMVYALEIIPYETLAAEMAEDFVTKARMAGSRIIFGQ
                     LSAILAGFAPMWLIGWLGRDNAATFFWMAAVFTGLFMVVAGLLYAFSWERARGAAPMP
                     PGRSAGQVLAGMVADLRATLQVRAFRLHLGMYLGGYISQDIFNATFTFFVLFALGGST
                     AAASGLLGSMYIVQLFAVGLAMQLVLRMGPAPAYRWAALAFAAGVVVLLGGWQAHWRV
                     GAEGLWLAIGLAGLGRGMLNYIPWATYNYMADVDEIITGQRREGSFAGVMTFVRKATQ
                     ALAVALVGMGLEAGGIRPAAAAQGPAAMAVLAAVLGAGTLAMLAGGVAVSRRFRLNGA
                     THGVLMHEIRHLRGGATGPSSAAAQAVVEDLTGCAYARLWGRAPR"
     gene            complement(10824..11936)
                     /locus_tag="VFF98_01125"
     CDS             complement(10824..11936)
                     /locus_tag="VFF98_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZU62256.1"
                     /translation="MAATTHSIPRADVLDAIARLTDNLVNIKDETGEFLLRLEDGRII
                     DTKGWNDWEWTHGVGLFGLWRYYEVTGDAATRRIIEEWFANRLAAGTPTKNINTVAPF
                     LTLANLYELSPDITYLPYLDSWAEWLMAPDGLPKTEEGGFQHIVYNDENPGEMWDDTL
                     MMSVLPLARIGQVLGRPHYVEEARRQFMLHVKYLVDRRTGLWFHGWTFDGRHNFAGAL
                     WARGNCWVTIAIPEFIEMLDLPEGDGLRAFLIETLAAQARALARLQDESGLWHTLLDD
                     PTSYLEASATAGFAFGLLKAVRKRYLPAEYADVALRAIKGVLAHIAPSGELTQVSFGT
                     AMGDSLQFYKDIALTSMPYGQSLAICALGEFLRTYI"
     gene            complement(11998..14364)
                     /locus_tag="VFF98_01130"
     CDS             complement(11998..14364)
                     /locus_tag="VFF98_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62257.1"
                     /translation="MQISSRRGRLLASAIGTLALAHQAQAQTTAPAAAPAASSGAIAD
                     IVVTATRRAERLQDVPVSVTAVTSALISRQNVRDINDLPKLVPGLTLNYGSQPGNNSI
                     NLRGIGTLTNGIAVESDVAIVVDDVPLGFQAEAFKDLVDIERVEVLKGPQSTLFGKSA
                     IAGVLNITTQAPTDHWTGHVTGLVTDDHEWRLGGTIAGPITQNLRVRLTAAHDSWDGN
                     IHNLTDGSRMNGSVGTTVTGKLVWDAGSRLTLSVSPRYNHTDTNCCVTPIRALTPGLY
                     YQGLSQLPASTVLAGINPNDTWNTNVRNDVRAGGISESYGVTWRANEKLEGGLLAGGT
                     LAYIGSVDHYHLRDYQDVDGTDSPFLLYYPASAPSGLDSGARLDGYFTVRSTTQELRL
                     TSGSGPLRYVAGLWFARNSLTRDLARGPVLQYVHYHATTVNETYSLYNDLAWDAAPRF
                     TLVGGFRLNRQLVDYTYANTTASPAFALGGHSSDNAITGKAGAQYHFTRDNMAYFTFS
                     TGYKGQAYDLSSVFNANIAAHSPVAPETARSYEIGSKNSFFHHRLQLNVTGFWTNYSG
                     FQTSSITYLPNGFALSLLQSVGKLRTRGVEMDFTAKPLPRVSLNGAGAFTDARITDFP
                     TGPCYTNQPYVTLASADSIPAPGQCGLLPSGTKIQNLKGGTMNNAPRWKFNIGGQYDL
                     PLEKGYGAFASFAYRWQSKVNFSLNQDPMTVQGAYGVFDATLGASADDGRYKLTFFVN
                     NLFNQHYAVNLGNTASGFSATGVAAQGLIWQPARDSFRYIGVKLDASF"
     gene            14541..15275
                     /locus_tag="VFF98_01135"
     CDS             14541..15275
                     /locus_tag="VFF98_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZU62258.1"
                     /translation="MQTKPLARRTLATELVEELRSQIEAGALVPGNRIPTEKDLVNSY
                     GVSRTVVREAVARLTAEGFLEPRQGSGVFVTMPPPAAFQVTHTELEDLVEVIRFLELR
                     QAVEVEMAGLAALRRSDEDVAAMHACAAQIGREFAAGKSSTEADEELHALIAKASGNQ
                     YFVRFIEFLELRMVPRRNLVTDLNEAQRAAYFSAVDGEHVALIEAIARQDSEAARAAA
                     RQHLENARRQIEDFIARRQPAGEVRA"
     gene            15338..16174
                     /gene="kduI"
                     /locus_tag="VFF98_01140"
     CDS             15338..16174
                     /gene="kduI"
                     /locus_tag="VFF98_01140"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HZU62259.1"
                     /translation="MFAKTYHATHPDMMDAASNQALRDRYLVGGMFATGEVRLNYSHN
                     ERFVIGGAVPGASPLQLPDQSEPASAAGHPFLERREAGIVNIGGAGAITVDGQRFEMG
                     NKECLYVPMGSREVLFEGEGARFYIASVPAHKACPIRHITLAEANPLARGDLANSNQR
                     TIYQLVIPGVCESAQLLLGLTVLEEGSVWNTMPPHLHDRRSEIYLYFDLPAPESRIFH
                     YMGQPEDLRHIVVANEEAVISPPWSVHMGSGTQKYAFIWAMGGENLDYTDMKVLDICQ
                     LK"
     gene            16182..16946
                     /gene="kduD"
                     /locus_tag="VFF98_01145"
     CDS             16182..16946
                     /gene="kduD"
                     /locus_tag="VFF98_01145"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HZU62260.1"
                     /translation="MSFDLTGRVAVVTGANTGIGQGIALALAAAGADIAAVGRTPAAD
                     TAERVQALGRRCALVQADLSSIAPVDAVVAQVLEQLGGLDILVNNAGIIRRADAVDFT
                     EEDWDAVVDTNLKSVFFLAQAAGRHMIARHAATGRRGKIINIASMLSFQGGIRVPSYT
                     ASKAGVAGLTKLLACEWAAHGVNVNAIAPGYIATANTAALQADAARNRQILERIPEGR
                     WGDPADIGGAAVFLASSAADYVQGHVLAVDGGWLAR"
     gene            16943..17977
                     /locus_tag="VFF98_01150"
     CDS             16943..17977
                     /locus_tag="VFF98_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZU62261.1"
                     /translation="MSGPVDATPSGPVVCFGEVLMRLSTPAGQLIADAPALDIAIGGA
                     EANVAVALAAMGHKARFLGRLPANALGLRAQAALAGAGVDVAHCPQAPGRMGLYVLET
                     GASLRPSAITYDRAGSAFASAAPGDFAFAQALEGAALLHLSGITPALGPGGVALARAA
                     IAAAQAAGVPVSFDCNFRANLWSAWDSDPRALLTELIGAASLLFANHRDMTLLLGRPF
                     SGDGPERRREAAEAAFAAFPRLQVMASTARTPLTQTHHRLAARVDLPTARHQTAEINI
                     TDIVDRVGSGDAFAAGVLSAWLEGGDAAAMAGRGLAMNALKHSVRGDWLRLPRSAIEG
                     FCPQPADVQR"
     gene            complement(18054..19466)
                     /gene="lpdA"
                     /locus_tag="VFF98_01155"
     CDS             complement(18054..19466)
                     /gene="lpdA"
                     /locus_tag="VFF98_01155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZU62262.1"
                     /translation="MAEQYDVIVLGSGPGGYVAAIRAAQLGLKTAIVERENLGGICLN
                     WGCIPTKALLRSAEVFHQMQHAKAYGLSATGVSADLAAVVARSRGVAKQLNQGVTHLM
                     KKNKVAVHMGVGKILKPGTLEVTGEKGTETLTAKHIIVATGARARDLPFAKADGERIW
                     TYRHAMTPKEMPTKLLVIGSGAIGIEFASFYNDLGAKVTVVEMLDRIVPVEDKDVSTF
                     LEKSLAKQGMTILTGTGVDGITTSASGIKAKIKGKDGKITEEDFSHVIVAVGVVANTE
                     NIGLEEVGIKTERGIIAIDEHGRTAVKGIWAIGDVTPGPWLAHKASHEGVTAAEAIAK
                     ELGNKEVHPHGLDRNNIPGCTYCHPQIASVGLTEAKAREAGHEVKVGNFPFIGNGKAI
                     ALGEPEGFIKTVFDAKTGELLGAHMIGAEVTELIQGYVVGKTLETTEQELMNTVFPHP
                     TLSEMMHEATLSAYGRAIHF"
     gene            complement(19472..19861)
                     /locus_tag="VFF98_01160"
     CDS             complement(19472..19861)
                     /locus_tag="VFF98_01160"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZU62263.1"
                     /translation="MEPLNLSPAMEPMIRVTAMPADANAYGDIFGGWLLSLMDMGAGL
                     MASRHSRGRAVTIAMDGVQFLKPVKVGDEVSVYGELRKVGRTSMTIAIEAWRRDRFQT
                     EAIKVTEAVFTFVAVDDSGRPRPIVQE"
     gene            complement(19881..21152)
                     /locus_tag="VFF98_01165"
     CDS             complement(19881..21152)
                     /locus_tag="VFF98_01165"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HZU62264.1"
                     /translation="MPIAIKMPALSPTMEQGKLARWLVKVGDEVHSGDIMAEIETDKA
                     TMEFEAVDEGVIVSIDVAEGTDGVAVGTIIAQLAGEDEDASAVPAAAAPAAKPAAAPV
                     AAAPVAAAAVAVKAPVAAGERVVASPLAKRIAAQKGVDLAGIAGSGPNGRIVKADVEG
                     ARPAAAAPAAAPVAAPVAAPAPVAASAGLPDFGIPHEDVALSGMRKTIARRLTESKQQ
                     VPHIYLTVDIKLDALLKLRGELNGALAARGVKLSVNDMLIKALGVALTQVPQCNVAFA
                     GDTLRQYARADVSVAVSIPNGLITPIITDAAGKGLARISTDMADLATRAKAGKLAPSE
                     YVGGTASLSNMGMMGIRQFEAVINPPQGMIMAIGAGEKRPWVLEDGSLGVATAMSATG
                     SFDHRAIDGADGAQLMAAFRELVENPLALLA"
     gene            21334..21789
                     /locus_tag="VFF98_01170"
     CDS             21334..21789
                     /locus_tag="VFF98_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZU62265.1"
                     /translation="MRVYLAILDETEEARAALRFAARRAAKTGGVVHILALVPRQPFV
                     AFAGVQATIEEEARARAEAQAAVAASSVLTEGGFPPHIEVREGEGIKVVREYLAAHAE
                     VAALVLGSARDGAPGPLVTHFSSHGIGDLPCPLMIVPGGLAEADIDRLS"
     gene            complement(21832..24180)
                     /locus_tag="VFF98_01175"
     CDS             complement(21832..24180)
                     /locus_tag="VFF98_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HZU62266.1"
                     /translation="MSRGPTELARGPRSVPGKLPQGLPSAQQVLDFIANAEEEVGKRE
                     IARAFGLKGQEKIQLKALLKDMAEEGLIDGKRTAYHRMGGVPRVCVLRVVEIEDAAPI
                     AVPDVWAPDEAAAPPRLRIVEPRGAGRGGKGPVMKGVGALKVGDRVLARTEELEHGWL
                     AHPMKKLPARAEQLMGVVEIDGAGKPWLSPIDKRERHAPGIADLGEAKEGQLVLAEPV
                     SRSPRAGVKVTAVLGDPLAPKAFSLIAIHKYGIPWNFSNAVLEEAEEAARQPLSAEQR
                     EDLTHLPIVAIDPSDARDHDDAIWAEPDEAEDNKGGWRAVVAIADVSFYVRSGGALDR
                     EARQRGNSVYFPDRVVPMLPEVLSADVCSLKAGAPRAAMVCHLQIDAGGTLKSWRFVR
                     AIVQIAEVVAYEDVQARIDEGRAEAHFVHLWEAWKALNKARERRDPLELELPERRVML
                     DDQGRILNIAPRERLDAHRVVEDFMIAANVAAAKALEAKVQPVVYRIHEPPTREKLIT
                     LKDYLATFGRKLALGQVITPELFNAMLRKIDDEAEKALIMEAVLRSQTQAYYGPRNAG
                     HFGLALGSYAHFTSPIRRYSDVLIHRALVDGYGLEQPAPKGHLPATSGLAARDRTDLA
                     PICDSISMAERRAMEAERETVDRYVAAWLSTRVGEVFECRITGVQRFGLFATIMGLGG
                     DGLVPISVLGEERFAHDEKAQALVGESTGTRFAMGDMLRLRLAEANPLTGALKFEPEE
                     GGGRIERRGAPAPDPSAQKRRGAHLVGRRGRPANIRHQGKRR"
     gene            complement(24161..25723)
                     /locus_tag="VFF98_01180"
     CDS             complement(24161..25723)
                     /locus_tag="VFF98_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZU62267.1"
                     /translation="MNQKTQNAPAIRHALSVTRAEDFAQWYQAVVSEGEMAEESGVRG
                     CMVIKPWGYGIWERIQKVMDARIKDAGVDNCYFPLFIPLSYFSKEADHVEGFAKEMAV
                     VTHHRLIGDGKGGLMPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWRDLPLMVNQWA
                     NVVRWEMRTRMFLRTSEFLWQEGHTAHATCADATAETLRALEMYRSFAEDVLAMPVVA
                     GEKPENERFPGAVATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDREGAQALA
                     HTTSWGVSTRLIGGVIMTHGDDDGLRVPPAIAPHQIIILPMLREDEGDEALLAYCEEL
                     RRALVSQSALAEPIRVLLDKRAGKATQKRWAWVKKGAPVILEIGGRDAANGQVSVLRR
                     DALWRADAKVNFTGQAKEDFVGEAAALLEDIQRSLHAEAAARHAANIRTGITTLAELA
                     TFFAEDQRYPGWVELNWSRPTGAALEQVVEQLKALKLTIRNTALDAAPAEGLCPFTGA
                     PAVERIYVARAY"
     gene            complement(25804..26397)
                     /gene="phaR"
                     /locus_tag="VFF98_01185"
     CDS             complement(25804..26397)
                     /gene="phaR"
                     /locus_tag="VFF98_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HZU62268.1"
                     /translation="MTENASAKDNSVIIKKYANRRLYNTQSSSYITLDHLARMTREGI
                     DFRVLDAKTGTDITHQILTQIIMEEEASGEQMLPVNFLRQLIGMYGNSMQGLIPHYLE
                     ASMEQFRANQMKLRKAFEDSMEANPLARLAQQNIAMFQAAAAAFMPGAEGGVPAKGMD
                     QPGEAAPTAAPAAGKEDLDVLRAQMLEMQKKLDSLTK"
     gene            complement(26498..27544)
                     /locus_tag="VFF98_01190"
     CDS             complement(26498..27544)
                     /locus_tag="VFF98_01190"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZU62269.1"
                     /translation="MVEAPAAPLRLLLDAEALAANWRLMDALSGSAQAGAAVKADAYG
                     LGALRATRVLAAAGCRDFFVAHWQEAAALLDVVPAEQIAVLHGPAGAQDIAFARATGV
                     RPVLNTMAQVRGWAAAGGGACHVMVDTGMSRLGLPAEALGDAGVQALAVDVLLSHLAS
                     AEEETPQNAAQLALWQQARGLVPHRRASLANSAGILLGADYHGDLTRPGLALYGGLAR
                     PELAGRIRQVARPQAALLQLRDVPAGAKVGYNATFTAPVPMRLGVVSLGYADGYLRCW
                     SGRGALLSGGRVLPVVGRVSMDLTIVDLSAAPDLAEGDWLDVAYDLAEASARVGLSPY
                     ELLTVLGRRFSRAA"
     gene            complement(27537..29159)
                     /locus_tag="VFF98_01195"
     CDS             complement(27537..29159)
                     /locus_tag="VFF98_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62270.1"
                     /translation="MTEPARHPDIQPSAADMRLVIAASSMGTVFEWYDFFIYGTLAGI
                     IGKTFFPAGNETLQLLLVWAGFAVGFGFRPLGAVLFGFLGDRLGRKYTFLVTVVLMGV
                     ATAGVGLVPPAASIGMAAPLIVIGLRVLQGLALGGEYGGAAIYVSEHARAERRGFFTS
                     FIQASVVGGFLLSLVVVLSCKAAFAPVQWAGWAWRVPFLISLLLLAVSMWMRLKLSES
                     PVFQAMREEGELAGNPFVESFTFPGNVKRIVVALFGVAAGMTVIWYTGMFSVLSYLKG
                     AMHLEDTAAELLVGVGGSLGAVLYIAAGALSDRVGRKLPIIASYVLTLLFVFPLFHGI
                     GALANPGLAEAARRAPVAVSGPACDYSPFASEQPSDCGKLLADLTALGVTYTRSDAAV
                     LSLTVGGKAQAIAAYPFAQKAARARMLQGWLGAEGYSFAKVTPGPGASVGIIALIVGL
                     VGLAALAFGPQAALLSEMFPPRIRYSSMSIPYHLGTGYFGGFLPLIASYIVARSGNPY
                     AGLWYTWGVVALALMVALWGLKGGRKQEFADG"
BASE COUNT         4690 a        10289 c         9564 g         4649 t
ORIGIN      
        1 attatcacgc agccgcaccg ccggggcccc gccagaacct gtgcatttcc gcccttttcg
       61 cgtttggcac ggctgttgct tttccggtgg atgatccatc atcgggagat gccacgcaga
      121 tgaccgccac cgcttcccag cccacaccgc cgccgcccgg cccgccagag gagatctggt
      181 tcacccgctg cccggtgccc accgccaccg gcatcgccta caagctgggc tggctgagcg
      241 aggcctttgc ggcagatggc attgccctgc gcaccctgca ggaatcgggc agcgagctgg
      301 cccgccacca ttacgaccac cgcctgccca cgctgatccg cgagggcggc aacatgctgg
      361 ctttcggcgc ccgcgcgcag ggcgagccga cccggctgat cggccttacc tggatcgagg
      421 aaagccagct gatcctcacc cgccccggca ccggcatcgc ccgcgccgag gatctgaagg
      481 gcaagcgcct ggcgctgccc ggctggaacc gcaaccccat ccccgaacat gtgcgcggca
      541 ccagcatcgc gcgcggcatg agcctggccg gctacaaggg cgcgctggcc tcggtcggcc
      601 tcacgcttga tgatgtgacg ctggtggaaa cgcccgatgc cacgcgcgag ctggccggat
      661 cgggccgcga catcgacggc gaagaccgcc gcctgcagcg cgggctgggc cggctgtggc
      721 cactgcaggc gctggtcgat ggccaggtgg atgcgatcta tgtgaagggc gccagcgcgc
      781 tggatgcggc gcgcgcggcc gggctggacg tggcggtgga cctcgacgcc atccccgacc
      841 ggcgctttcg cgtcaacaat ggcacgccgc ggccgatcac cgtgcaccag agcctgatcg
      901 acgatcattt cgactatctg gtgcgcttcc tcgccatcac cctgcgcgcg gccgactggg
      961 tggcccgcca gcccgaccag ctgcgcggca tcctcgcctt cgagacagcc gccagccccg
     1021 aggcggtgaa ccacgcctat gcccgactgc acgagggcct gcgccccacg ctggatgccg
     1081 accgcctggc cctgttcgac cagcagaaga ccttcctgtt cacccacggc ttcctcgatg
     1141 ccgatttccc cctggccgac tggatcgacc cgcgcccgct ggaagcggcc atcgcgctga
     1201 acgccaacca gccccagccg ctgcgcgaac ctgcgcccct gcccgcctga acgcctgaac
     1261 ccccgacagc agaaaaggac cgaccatgac ccacggactt tccaaggaaa ccatcgcctt
     1321 cgtgcgtgaa accgaagccg atgcgcgcaa ggccttccgc tggctgcgcg aaacccagac
     1381 cacctcgccc tcggccacgg tgggctttgc cgtgcgcgtg cccggcgaag acaagatcgt
     1441 caccctcgcc tatgccggcc tgtgggccga ggacccggac gcgccgatca ccgaagtgat
     1501 cgactttgac ggcaacacct attggggcgg caagcccagc ggcgaatcgc gctatctgaa
     1561 gatcttcagg aaatacgagg attttcaggc gatcagccat gtccacgcgc ccaacctcgg
     1621 cgcctattcg caggcccaca gcgagctgcc gctgctttat gtgcccaacc ggcgcttccg
     1681 caccaccacc cacctgccgg tctatatcaa ccgccgccag agcgaggccg atttcatcct
     1741 cgaggcgatc gccgccgaca agcagacgcc cggcatcgtc gaggccaatg gcggcgccac
     1801 ggtgtggagc ttcaagggcc tgcgccatct ggtgcagaac atcatcctgc tggaagaggg
     1861 cgcccgcttc caggtgctgg ccgcagcgct gggcggctcg cgccccttcg gcccgggcgt
     1921 gctggagcag cagtggaaga tcggcggcct ggtgccagcc gatgcccatg tgaccgacga
     1981 cggccagatc ctgctcgcca accccgcccc ccaggcggcc gaataagcct tcctgccccc
     2041 ggcgcccatg gtgccggggc ccctccctgc cggagcccac gatcatgacc agcccgaagc
     2101 gccagctggc gctcgccgtt ttcctcaccc gccacggcca tcatcccggc gcctggcgcc
     2161 agcccggcag cgcgcccacc ggcctgcccg atttcgccca ctgggcaagg ctggtgcaaa
     2221 ccgccgagcg cggcaagctg gatgccgcct tcttcgccga tttcgccggc atggcggggg
     2281 ataacaccag gggcattgcc cggcgccacg ccttcctcga tttcgagccg ctgacggcga
     2341 tctcggcgct ggccagcatc acccggcata tcggcctgat cgccacggtg aacaccaatt
     2401 tcgagcagcc ctatgcgctg gcccgccgcc tggcctcggc cgatcacatc agcggcgggc
     2461 gcgtgggctg gaacatcgtc tcctcgctgg atccgggcgc ggccaaaagc tttggcatca
     2521 cccagccggc cacccatgcc gaacgctatg cccgcgccga tgaattcgtg gcgctggcca
     2581 aggccctgtg ggacagctgg gaagacgatg ccttcgaggg cgccgacaag gccagcggca
     2641 tctatctcca ggccgacaag gtgcatccgg tgcattatcg cggcgcctat tatcaggccg
     2701 atgcggtgct cgatgtgccg cgctcgccgc agggctatcc cgcctttgcg caggccggca
     2761 attccgaggt gggccgcgat tttgccgcgc gccatgccaa catgatctat gccgccgccc
     2821 agttcatcga ggacgcgcag gatttctacg gtgatgtgaa gcgccgcgtg gtggcccatg
     2881 gccgccaccc cgacgagctg aaggtgatgc ccggcctctc cttcaccctt ggcagctctc
     2941 gcgccgaagc gcaggaccgc tttgccgcgc tggaagaggc agtggatttt tccggcgatc
     3001 tcaacctgat ggggcaggat gtctcggcct atccgctcga cgggccgctg cccgacctgc
     3061 ccgagcccga aaacggcaag ggccgctggc gccagctgac cgcgcttgcc cggcgcgaga
     3121 acctcaccat ccgccagctg ttcctgcgct tcaacgcggt gcgcggccat cgcgtgctgc
     3181 tgggcaccgc ggccgatatc gccgatggca tggaggaatg gttcaccgcc ggcgctgccg
     3241 atggcttcaa cctgatcccg ccggtgctgc ccacagggct ggatgccttt gtcgatcagg
     3301 tggtgcccga attgcagcgg cgcggcctgt tccgcaccga atatgaaggg cgcaccctgc
     3361 gcgatcatct gggccttgcg cgcccggcca atccgcatac cgcccgccag actgtgacag
     3421 agccggcctg agaccggcca ggggagaacg accatgaccc agaaccatct cgacaccttg
     3481 tggtacactc gctgcccggt gcccaccggg cttggcatcg ccatccagca gggctggctg
     3541 ggcgaggcct tcgcccagcg cggcacgcaa gtgaaatcgc tgctggaatc gccggatttc
     3601 gccgtgcgcg aatcgcattt cagccacacg ctggccaatt ccgtgcgcca tggcggcagc
     3661 attccggcga tcagcgcctt tgcccgcggg cgcgacacgc ggctgatcgg cctgtcgtgg
     3721 gccgatgaga cacagctcat cctcgcccgg ccggaaagcg gcatcaccac cgtgcgcgaa
     3781 ctgaagggcc gccgctttgg cctgcccaac tggaagcagg tggagattga tttctcccgc
     3841 gcccaggcga tccgcgggct ggaaaacgcg ctgcgcctcg aagggctgga agtggccgat
     3901 gtggagctgg tgaaccagga catcgacagc cattattccg aaggcgccgc gcgcagcatc
     3961 gccggcaccg tcaactggac cgggcgcacc ccttcgcgcg cgcgggccaa caacctcgag
     4021 gtgatcgccc tgctgcgcgg cgaggtggac gcgatcttcc tgaagggggc caatggcgcg
     4081 caggtcgccc atcagttcgg cctgaccacg ctgatcgaca ccggcgccca tcccgatccg
     4141 ctggtgcgtt cgaacaatgg cacgccgcgc acgctggccg tggatggcca gctgatcgag
     4201 cagcatttcg aggccgccac caccatcgcc gagcaagtgc tgcgcgccga agcctgggcc
     4261 caccgccacc ccgaggaggt gcgccgctat ctgggccgcg aggccaatgc cagcgagttc
     4321 tgggtggaag cggcctatgg cgccgatgcg catctgaagc tcaacaccga tctggccgaa
     4381 caatcggtgg cagccctgca ggatttcacg cagttcctgc accgctgggg cttcattccg
     4441 cgcagctttg ccgtggccga ctggatcgac ccgcgcccgc tggccgaagc gcgcagccgg
     4501 ctgcaacagg ccgccccgct ggccgcggcc gaataggcca tgggcaagcc actggcggtg
     4561 cctggcgcgc agccggcacc gcggcaggcg ggcgcgggcg ggcatccctt cctgcgctgc
     4621 cttgctatct atgccgaaat gccgcgccgc ttcctgctca ccgcactgct gctggggctg
     4681 gtgaatgcgg ccatgctggg ccagcaggtg ctggtgggcc gggcggtgca ggaggccggg
     4741 cgcgggcaac tggtggccag gctgccgggc ggcgggctgg attatcaccg cgccggctgg
     4801 tttgccgcgg tgctgctggg gctggcggcg ctgcgggggg gcttgcaact gggcgcgggg
     4861 ctgctctcgc tgtcgatcgg gcaggaactg ctgacgcggc tgcgcctgcg cattctggcg
     4921 cagatccagc ggctggatct ggcctatcac tggcgccacg gcgtgggcga gatggtgacg
     4981 cgcatgaccc gcgatgccga caagctgcgc gacgggctca tcaacttctg gcgccagatg
     5041 ttcgagacgg ggctggtggt ggtgctctcg ctggggctgc tggcgggctg gtcggtgtgg
     5101 ctggcgctgg tgccgggggg gctgttcgtt ctgggcatct ggctgctggc gcgcgagacg
     5161 ggcacgctgg tcgcgctcga ccggcggatc ggcgatgcct atgatgccgt cacgcaggat
     5221 ctgtccgagg gcgtcaatgg cgtgcgggtg atcaaggcct ttgccctggg ccaggcccgc
     5281 gcccgccgct ttgccgcctt catcgacctg ttccgccagg aagcccgcgc ggcgctcgcc
     5341 tatgcctccc ggcgcgtgcc gctgccgcag gcgatcatcg cgctgggcca tgcctgggtg
     5401 ctgggctgcg gggcggtgct ggtggcgcgc ggcagcctgg ggctgggcgc gctggtggca
     5461 gcgctgctgg tggtcaacaa tctggtgctg cgggtggaaa atatcggccg gctgctgcag
     5521 gtggtggccg atgccagcgc ctcggccgcg cgcatctggg atgtgctgga tgcccggccg
     5581 gcgatcctgg gcggccccgc ccccgtgccg gccgggcccc tgggcctgcg gctggaacag
     5641 gtctcggtgg cgccgctggg cgatggcggg cgggtgctgc atgatgtctc gctggcgctg
     5701 gcgccgggcg aggtggtggc gctggtgggc cccaccgggg cgggcaagag catccttgcc
     5761 agcctgctgc cgcgccttgt cgaggtggac gagggccgtg tgctggtggg cagcgatgcg
     5821 gccggctggc aggatgtgcg cgggctggat ctggccgagc tgcgccgccg ggtgcatgtg
     5881 ctgccgcagg aaagcttcct cttttccgac acgctggcgg ccaatctgcg cctcagcgcc
     5941 cccgcggccg aggacgccac gctgatcgcc gcgctggagc aggcggccgc cggcgatgtg
     6001 ctgccccgcc tggccgaagg gctggagacg cggctgggcg atcgcggggt cacgctgtcg
     6061 ggcgggcagc gccagcgcat ctgcctggcc cgggcgctgc tgtcgggcgc cgatatcctt
     6121 gtgctcgacg atgccaccag cgccctcgat gccgccaccg aacaacgcgc gctggccaat
     6181 atccgcgccc tgcgcggcag cggcgcggcg cccacggtgc tgatcgtctc cagccgcctg
     6241 tccaccattc tggcggcaga ccgggtggtg atgctggccg ccgggcgcat cgtcgcccag
     6301 ggcccgcacg cgcagctggc cgccggcaat gccgcctatc gcgccttgat ggggatctga
     6361 gatgaacggg tttcgcggcg atagcgccct gtcggaaatc gagctggaag agcggctggc
     6421 gcaaaagcgg ctggggcgcg gcgtgggcgg ccggctgtgg ccgctggtgg cgccggtgcg
     6481 gcggctgatc gcggcggtgg tggcgatcga gctggcgcag gtggcggtga tcttcgcccg
     6541 cccggcgctg atcgccctcg tcatcgacag gggcttgcgc catggcctgc agccggcgct
     6601 ggtgggctgg gcctgcgcgg ggctggcggc gtgctgggcg gcgcgtttcg ggctgggggg
     6661 cctgtcgcaa tatgccgcgg gcctggcggc gatccgcgtg ctgaacgacc tgcgcgcccg
     6721 cgtcttcgcc catgtgcaga cgctgtccgt cggctatttc gaccgcaccc gcgccggccg
     6781 catcatcgcc cgcgccgacc gcgatgtcga tacgctggag ccgctgctga tccagggccc
     6841 gcccgaactg ctgtcggcgg tgttgcggct ggtggtggca ggcggactgc tgtggcaggt
     6901 ggcgccggcg ctgctgctgg ggctggccgg cgtggtgccg ctgctgctgg gggcaacggc
     6961 gctgttcaag cgcatttcgc aggccagctg ggcgcaggtg gccgaatgcc gcagccgctt
     7021 caccgcccat ctggtggaaa gcgtggcggg cgtgcggctg atccgccagg ccggccgcgc
     7081 ggcggaaaac ctggccgcct atcgccgcct gctcgatgct ttcaacaccg cgctcatccg
     7141 cggcaatctg cgctcgggct ggtttgcgcc attcaccggc ctgctcacca cggcaggggt
     7201 ggtgctggtg ctggtgatcg gcgggcgggg cgtggcgctg ggcacgctca gccttggcca
     7261 gctcacgcag agcctgttct acgtctatct gttcctggcc cccttgcagg aattttccga
     7321 cctgttcgag cggctggcca atggcaccgc ctgcgcccag cgcatcttcc tgctgctgga
     7381 taccgccccc gacatcgccg atgccgccga tgcgcgcgat ctgccgcgcc tggccggcgc
     7441 catccgcttt gccggggtgg gctttggcta tgtgccgggg cggcgggtgc tgcaggggct
     7501 ggatctggcc atagcgccgg gcgaagtggt ggcgatcgtg ggccccaccg gccatggcaa
     7561 gagcacgctg gtgcagctgc tcacccgctt ctacgatgtg gacgaaggcg ccatcctgct
     7621 cgacgggcac gatatccgcg ccatttccga ggccagcctg cgccgccagg tgggcgtggt
     7681 gctgcaggat aacgtgctgt tcggcggcag cgtgctcgac aatctgcgcc tggcccggcc
     7741 cgctgccagc gatgccgaac tgatcgccgc cgcgcgcgaa ctgggcgccg acgcggtgct
     7801 ggaagccctg ccccatggct atcacacgca ggtcggcgct cttggcgggc agctcagcca
     7861 ggggcagcgc cagctggtct gcctggtgcg cgcctatctc gccgatccgg ccgtgctggt
     7921 gctcgacgag gcgaccagcg cggtcgatgt cgccaccgaa cagcgcatcc agcgcgccct
     7981 gcgccgcatc accgccgggc gcaccagcct catcatcgcc caccgcctcg ccaccatccg
     8041 cgaggcagac cgcatcgccg tgatccgcga aggcgcgatt gtggaactgg ggccgcatga
     8101 cgcgctgctg gcgcgcggcg ggcattatgc cgcgctgtat gccgcctatg cggcgccccc
     8161 tgcgcccttg cctgcgatgg catagatagg cgccatccag ccttcgggtt tgccatggcg
     8221 cgcagcaccg ctaggatcgc gccatgacag acctgttcga ccttaccggc caatgcgcgc
     8281 tggtcaccgg ctccacccgc ggcattggcc tggcgattgc aaaagcgctg atcgccgccg
     8341 gtgcaaaagt ggtgatctcc agcgaggatg ccgccgatac cgcccgcgtg gcggccgagc
     8401 tggccatgcc cggcatcgcc tgcgacatgc gcgaggaggc cgcccttgaa ccgctggtgg
     8461 caggcacggt ggcagcgctg ggcgggctcg acattctggt gtgcaatgcc ggcatcaccg
     8521 gccgcccggg ccccttcgcc agcctcgaca tggccgatta cgcgcgggtg atggccatca
     8581 acctcacagc gcaggtgctg ctgtgcaacc acgccctgcc gcatatcgcc gcgcgcgggg
     8641 gcggcagcgc cattctggtg gccagccttg ccggcctgcg cggcaatggg gcgatcaacg
     8701 cctatgcgct ggccaaggcc ggggtggccc agctggcacg caatctggcg gtggaatggg
     8761 ggcccagggc cgtgcgcgtc aatgccctgt cgcccggctt catcgccacc gaactgtcga
     8821 aaccgctgct ggagaacgaa gccttcatgg cccgccgcat ggggatgacg ccgctgcgcc
     8881 gccccggccg gccggaagag attgccggcg cggcggtgtt tctggcctct gctgcgggcg
     8941 gttttgtcac cgggcacaat ctcgtcgtcg atggcggcac gctgatcacc gacggcagct
     9001 gagcctttag ggcagcagga acacttctgt cagcggccag ctgcgggggc tgtcatcggc
     9061 cgccacttcc atcagcggcg ccatggccgc ccaccagcgg cgcatcaccg gcagatcggg
     9121 cagcgcatcc atgccatggc cctcctcgcg cagcagggtg gcaaacagca gatccgtgtc
     9181 ggcatcgtgg aagatgcggt aatcatggat gccggccgcc ttcagcgcca tggccagctc
     9241 tggccagatc tcgtcgtggc ggcggcgata ttccgccagc atgcccggct tcagccgcat
     9301 gcgaaaggca atgcgttcca tcctcgtatc tcctcgtcag cgcggggcgc ggccccacag
     9361 gcgggcatag gcacagcccg tcagatcctc gaccactgcc tgtgccgccg ccgagctggg
     9421 ccctgtggcg ccgccgcgca gatggcggat ctcgtgcatc agcacgccat gggtggcgcc
     9481 attcaggcgg aaacgccgcg acaccgccac cccgcccgcc agcatcgcca gcgtgcccgc
     9541 gcccagaacg gccgccagca ccgccatcgc cgccggccct tgcgccgctg cggccgggcg
     9601 gatgccgccc gcttccagcc ccatgcccac cagcgccacg gccagcgcct gggtggcctt
     9661 gcgcacaaag gtcatcacgc cggcaaagct gccctcgcgc cgctggccgg tgatgatctc
     9721 gtcgacatcg gccatgtagt tgtaggtggc ccaggggatg tagttcagca tgccccggcc
     9781 aagccctgcc agcccgatcg ccagccacag cccttcggcg cccacccgcc aatgcgcctg
     9841 ccagccgccc agcagcacca caacgcccgc cgcaaaagcc agcgccgccc agcgataggc
     9901 cggcgccggc cccatccgca gcaccagctg catggccagc cccacggcaa acagctgcac
     9961 gatatacatg ctgcccaaaa ggcccgaggc cgccgccgtg ctgccgccca gcgcgaacag
    10021 cacgaagaag gtgaaagtgg cgttgaaaat atcctggctg atatagccgc ccagatacat
    10081 gcccagatgc agccggaagg cgcgcacctg caaggtggcc ctcaggtctg cgaccatgcc
    10141 ggccagcacc tgcccggcag agcggcccgg cggcatcggc gccgcgcccc gcgcgcgttc
    10201 ccagctgaag gcatagagca gccctgccac caccatgaac agccccgtga acaccgccgc
    10261 catccagaag aaggtggcgg cattgtcgcg ccccagccag ccgatcagcc acatcggcgc
    10321 aaagcccgcc agaatggccg acagctgccc gaagatgatg cgcgatccgg ccatgcgcgc
    10381 cttggtgacg aaatcctccg ccatttcggc cgccagcgtt tcatagggga tgatctccag
    10441 cgcatagacc atctcgaagg cgacatagga gacgagataa taccagaagc cctgccccgg
    10501 cagccacatc agggcaaagc tgggcagcag cgggatcgcc gccagaatga acacccgccg
    10561 ccgcccgaag cgccgcccca gcccatgcgc gcccatatga tccgacaggt gcccgatcaa
    10621 cggcgagagc accgcatcga tcagccgggc cgccgcgaaa atcagcgccg cctgcccggc
    10681 cgacagcccg caaaagcgcg tgtagaacag cagcagccag cccgagacga tcgccatcga
    10741 gcctgccccc agcgcatcat tggcgccata ggccagatag ttgaccagcc gcaccggccg
    10801 cgcggcggaa agcggcggcg ccatcagatg taggtgcgca ggaactcgcc cagcgcgcag
    10861 atggccaggc tctgcccata gggcatggag gtgagcgcga tgtccttgta gaattgcagg
    10921 ctgtcgccca tcgccgtgcc gaagctcacc tgcgtcagct cgccgctggg ggcgatatgg
    10981 gccagcacgc ccttgatggc gcgcagggcc acatcggcat attccgccgg cagatagcgc
    11041 ttgcgcaccg ctttcagcag cccgaaggca aaaccggccg tggccgaggc ttccagatag
    11101 gaggtcggat catcgagcag cgtgtgccac aggccgcttt catcctgcag gcgggccagc
    11161 gcccgcgcct gggcggccag cgtctcgatc aggaaggcgc gcaggccatc gccctcgggc
    11221 aggtccagca tctcgatgaa ttcggggatg gcgatcgtga cccagcaatt gccgcgcgcc
    11281 cacagcgctc cggcgaaatt gtgccggcca tcgaaggtcc agccgtggaa ccacaggccc
    11341 gtgcgccgat cgaccagata tttgacatgc agcatgaact gccgcctggc ctcttccaca
    11401 taatgcggcc ggcccagcac ctggccaatg cgcgccagcg gcagcacgct catcatcagc
    11461 gtgtcgtccc acatctcgcc ggggttctcg tcattgtaga cgatgtgctg gaagccgcct
    11521 tcctcggtct tgggcaggcc atcgggggcc atcagccatt ccgcccagct gtcgagatag
    11581 ggcagatagg tgatgtcggg gctgagctcg taaaggttgg ccagggtgag gaagggggcc
    11641 acggtgttga tgttcttggt gggcgtgccg gcggccaggc ggttggcaaa ccattcctcg
    11701 atgatgcggc gggtggcggc atcgccggtc acctcgtaat agcgccacag gccgaacagg
    11761 ccaacgccat gggtccattc ccagtcgttc cagcccttgg tgtcgatgat gcggccatcc
    11821 tccagccgca gcaggaattc gccggtctcg tccttgatgt tgacgagatt gtcggtcagc
    11881 cgggcgatcg cgtcgagcac atcggcgcgg gggatggaat gggtggtggc agccatggcc
    11941 tgctccgctt ggggggatgg aaaccggcag ggacagcgct gcccctgccg caacatatca
    12001 gaagctggcg tcgagcttca cgccgatata gcggaaactg tctcgcgcgg gctgccagat
    12061 cagcccttgc gcggcaacgc cggtggcgct gaagccggag gcagtgttgc ccaggttcac
    12121 cgcataatgc tggttgaaca ggttgttgac gaagaaggtc agcttgtagc ggccatcatc
    12181 ggcgctggcg ccaagggtgg catcgaacac gccataggcg ccctgcaccg tcatcggatc
    12241 ctggttgagc gagaagttca ccttgctctg ccagcgatag gcaaagctgg caaaggcgcc
    12301 atagcccttc tccagcggca gatcatactg gccgccaatg ttgaacttcc agcgcggcgc
    12361 gttgttcatc gtgccgccct tcaggttctg gatcttggtg ccgctgggca gcaggccgca
    12421 ctggccgggc gcgggaatgc tgtcggcgct ggccagcgtg acatagggct ggttggtata
    12481 gcacgggccg gtgggaaaat cggtgatcct ggcatcggtg aaggcccccg ccccgttgag
    12541 gctgacccgg ggcagcggct tggcggtgaa gtccatctcc acgccgcggg tgcgcagctt
    12601 gcccaccgat tgcagcagcg aaagcgcaaa gccattgggc agataggtga tcgaggaggt
    12661 ctggaagccg ctgtaattgg tccagaagcc ggtcacgttc agctgcaggc gatggtggaa
    12721 gaagctgttc ttcgagccga tttcatagct gcgcgcggtt tccggcgcca ccggcgaatg
    12781 ggcggcgata ttggcgttga acacgctcga caggtcgtag gcctggccct tgtagccggt
    12841 cgagaaggtg aaataggcca tgttgtcacg cgtgaagtga tattgcgcgc cggccttgcc
    12901 ggtgatggca ttgtcgctgc tgtggccgcc cagcgcgaag gccggcgagg cggtggtgtt
    12961 ggcgtaggtg tagtccacca gctggcggtt gaggcggaag ccgcccacca gcgtgaagcg
    13021 gggcgccgca tcccaggcca gatcgttgta gagcgaatag gtctcgttca ccgtggtggc
    13081 gtgatagtgc acatattgca gcaccggccc gcgggcgaga tcacgcgtca gcgaattgcg
    13141 ggcaaaccac aggccggcca cgtagcgcag cgggcccgaa cccgatgtca gccgcagctc
    13201 ctgcgtggtg gagcgcacgg tgaaatagcc atccagccgc gcgccgctat ccagccccga
    13261 aggcgccgag gcgggataat agagcaggaa gggggaatcg gtgccgtcca catcctgata
    13321 atcgcgcagg tgatagtgat cgaccgagcc gatataggcc agcgtgccgc ccgccagcag
    13381 cccgccctcc agcttttcat tcgcccgcca tgtcacgcca tagctttcgg aaatgccgcc
    13441 ggcgcgcaca tcgttgcgca cattggtgtt ccacgtgtcg ttggggttga tgccggcgag
    13501 cacggtgctg gcgggcaatt gcgacaggcc ctggtaatag agccccggcg tcagcgcgcg
    13561 gatgggggtg acgcagcaat tggtatcggt gtggttgtag cgcggcgaga ccgacagggt
    13621 gagccggctg cccgcatccc acaccagctt gccggtaacg gtggtgccca cgctgccgtt
    13681 catgcggctg ccatcggtga ggttgtggat gttgccatcc cacgaatcat gggccgcggt
    13741 cagccgcacg cgcaggttct gggtgatggg accggcaatg gtgccgccca gccgccattc
    13801 gtggtcgtcg gtcaccaggc ctgtcacatg gccggtccag tggtcggtgg gggcctgggt
    13861 ggtgatgttc agcacgccgg caatcgcgct cttgccgaac agcgtgcttt gcgggccctt
    13921 cagcacttcc acccgctcga tatcgacgag atccttgaag gcctcggcct gaaagcccag
    13981 cggcacatca tccaccacga tcgccacgtc ggattccacg gcgatgccgt tggtcagcgt
    14041 gccgatgccg cgcaggttga tcgaattatt gcccggctgc gaaccgtagt tcagcgtgag
    14101 gcccggcacc agcttgggca aatcgttgat atcgcgcacg ttctggcgcg atatcagcgc
    14161 gctcgtcacg gccgtgaccg acaccggcac atcctgcagg cgctcggcgc ggcgggtggc
    14221 ggtgacgacg atatcggcaa tggcaccgct ggatgcggcg ggcgcggccg caggcgctgt
    14281 ggtttgcgcc tgcgcctgat gcgccaaagc cagcgtgccg atcgcagatg cgagcagccg
    14341 cccacgacgg gatgaaatct gcataaccct cacagcccct ctcctcggga tgtcgcttca
    14401 aaccggtctt tgccggacag gcagattcgt agcgaagaaa aatgaggatg acaacaggtc
    14461 atatgatgaa ttatggatgt ctccgtcagc cggcaccaaa ggggtggcca aggcggcccc
    14521 aaccgggata gggaatgagg atgcagacca agccactggc caggcgcacg ctggccacgg
    14581 aacttgttga ggaattgcgc tcgcagatcg aggccggcgc gctggtgccc ggcaaccgga
    14641 tccccaccga gaaggatctg gtgaacagct atggcgtcag ccgcacggtg gtgcgcgagg
    14701 cggtggcccg gctcaccgcc gagggctttc tggaaccgcg ccagggctct ggcgtgttcg
    14761 tcaccatgcc gccgccggcc gccttccagg tcacccatac ggagctggag gatctcgtcg
    14821 aggtgatccg cttcctcgaa ctgcgccagg cggtggaagt ggaaatggcc ggccttgcgg
    14881 ccctgcgccg cagcgacgag gatgtggccg cgatgcacgc ctgcgccgcc cagatcggcc
    14941 gcgaatttgc cgccggcaaa tccagcaccg aggccgatga ggagctccac gcgctgatcg
    15001 ccaaggcttc gggcaaccag tatttcgtgc gtttcatcga atttctcgaa ctgcgcatgg
    15061 tgccccgccg caacctcgtc accgatctca acgaggccca gcgcgccgcc tatttcagcg
    15121 ctgtcgatgg cgagcatgtc gcgctgatcg aggccatcgc ccggcaggac agcgaagccg
    15181 cccgcgcggc agcccgccag catctggaaa acgcccggcg ccagatcgaa gatttcattg
    15241 cccgccgcca gccggccggc gaggtgcgcg cctgagcctt gcgggccggc gcgcgcctcc
    15301 actgcccccc acaacatcat tccgaaaggc tgctgccatg tttgccaaga cctatcacgc
    15361 cacccatccc gacatgatgg atgccgcatc caaccaggca ttgcgcgatc gctatctggt
    15421 gggcggcatg ttcgccacag gcgaggtgcg cctcaactat tcgcataacg agcgtttcgt
    15481 gatcggcggc gcggtgcccg gcgccagccc gctgcaactg cccgaccaga gcgagccggc
    15541 ctcggccgcc ggccacccct tcctcgaacg gcgcgaggcc ggcatcgtca acatcggcgg
    15601 cgccggcgcc atcaccgtcg atggccagcg cttcgaaatg ggcaacaagg aatgcctcta
    15661 tgtgcccatg ggcagccgcg aggtgctgtt cgagggcgaa ggcgcgcgct tctacatcgc
    15721 cagcgtgccg gcccacaagg cctgccccat ccgccacatc accctggccg aggccaatcc
    15781 gctggcgcgc ggcgatctgg ccaattcgaa ccagcgcacg atctaccagc tcgtcatccc
    15841 cggcgtgtgc gagagcgcgc agctgctgct tggcctcacc gtgctggaag agggcagcgt
    15901 gtggaacacc atgccccccc acctgcacga ccgccgcagc gagatctacc tctacttcga
    15961 cctgcccgcc cccgaatcgc gcatcttcca ctacatgggc cagcccgaag acctgcgcca
    16021 catcgtggtg gccaatgagg aagcggtgat cagcccgccg tggtcggtcc acatgggctc
    16081 gggcacgcag aaatatgctt tcatctgggc aatgggcggg gaaaacctcg actataccga
    16141 catgaaggtc ctcgatatct gccagctgaa gtgagggcgc gatgagcttc gatctcaccg
    16201 gccgggtggc cgttgtcacc ggcgccaaca ccggcatcgg ccaaggcatt gccctggcgc
    16261 tggcggcggc cggcgccgat attgccgctg tgggccgcac accggctgca gacaccgccg
    16321 aaagggtgca ggcgcttggc cgccgctgcg cgctggtgca ggcagacctg tcctccatcg
    16381 cccctgtgga cgcggtggtg gcacaggtgc tggaacagct gggcgggctg gatatcctcg
    16441 tcaacaatgc cggcatcatc cgccgcgccg atgcggtgga ttttaccgag gaggactggg
    16501 atgcggtggt ggacaccaac ctcaaatcgg tgttcttcct ggcccaggcc gccggacgcc
    16561 acatgatcgc ccggcatgcc gccaccggcc ggcgcggcaa gatcatcaac atcgcctcga
    16621 tgctcagctt ccagggcggc atccgcgtgc ccagctacac cgccagcaag gcgggcgtgg
    16681 ccgggctgac aaagctgctg gcctgcgaat gggcagcgca tggggtcaac gtcaacgcca
    16741 ttgcgcccgg ctatatcgcc accgccaaca ccgccgccct gcaggccgat gccgcccgca
    16801 accgccagat cctcgagcgc attcccgagg gccgctgggg cgatccggcc gatatcggcg
    16861 gggcggcggt gttccttgcc agctccgccg ccgattatgt gcagggccat gtgctggccg
    16921 tggatggcgg atggctggcc cgatgagcgg cccagtggac gcaacgccga gcggccccgt
    16981 cgtctgcttc ggagaggtgc tgatgcgcct ttccaccccg gccgggcagc tgatcgccga
    17041 tgcgccggcg ctggatatcg ccattggcgg agccgaggcc aatgtggcgg tggcgctggc
    17101 ggcgatgggc cacaaggccc gcttcctcgg caggctgccg gccaatgcgc tgggcctgcg
    17161 ggcgcaggcg gcgctggccg gcgccggggt ggatgtggcg cattgcccgc aggcgcccgg
    17221 ccgcatgggg ctttatgtgc tggaaaccgg cgcctctctg cgcccttcgg cgatcaccta
    17281 cgaccgcgcc ggcagcgcct ttgccagcgc cgccccgggc gatttcgcct ttgcccaggc
    17341 gctggagggc gccgcgctgc tgcatctgtc gggcatcacc ccggcgctgg ggccgggcgg
    17401 cgtggcgctg gcgcgcgcgg ccatcgccgc ggcgcaggca gccggcgtgc cggtctcctt
    17461 cgactgcaac ttccgcgcga acctgtggtc tgcctgggac agcgacccgc gcgccctcct
    17521 taccgaactc atcggcgcgg ccagcctgct gtttgccaac caccgcgaca tgacgctgct
    17581 gctgggccgc cccttcagcg gcgatggccc cgagcgccgg cgcgaggccg ccgaggccgc
    17641 ctttgccgcc ttcccccggc tgcaggtgat ggcctccacc gcccgcaccc ccctcaccca
    17701 gacgcaccac cgcctggccg cccgggtgga cctgcccacc gcccgccacc agaccgccga
    17761 gatcaacatc accgatatcg tcgaccgggt gggcagcggc gatgcctttg ccgccggcgt
    17821 gctctcggcc tggctggaag ggggcgatgc cgccgccatg gccgggcgcg gccttgcaat
    17881 gaacgcgctc aaacattccg tgcggggcga ctggctgcgc ctgccgcgca gcgccatcga
    17941 aggcttctgc ccccagcccg ccgacgtgca gcgctgaacc tgccgcgcgg ggcaggcaaa
    18001 aggggcgcct ccccggccgg gaggcgcccc tttcctgtgc cgctatccgg cgatcagaag
    18061 tggatcgccc ggccataggc cgacagcgtt gcctcatgca tcatttccga cagggtggga
    18121 tgcgggaaca ccgtgttcat cagctcctgc tcggtcgtct ccagcgtctt gccgacgaca
    18181 tagccctgga tcagctcggt cacttccgcg ccgatcatgt gggcgcccag cagctcgccg
    18241 gtcttggcgt cgaacaccgt cttgatgaag ccctccggct cacccagcgc gatggccttg
    18301 ccattgccga tgaaggggaa attgcccacc ttcacttcat ggccggcttc cctggccttg
    18361 gcctcggtca gccccacgct ggcgatctgc gggtggcaat aggtgcagcc ggggatgttg
    18421 ttgcgatcga ggccatgggg gtgcacctcc ttgttgccca gctccttggc gatcgcctcg
    18481 gcagcggtca cgccctcatg gctggccttg tgggcaagcc agggccccgg ggtcacatcg
    18541 ccgatcgccc agatgccctt cacggcggtg cggccgtgtt cgtcgatggc gatgatgccg
    18601 cgctcggtct tgatgcccac ctcttcgagg ccgatgttct cggtgttggc gacaacgccc
    18661 accgccacga tcacatggga gaaatcctcc tcggtgatct tgccatcctt gcccttgatc
    18721 ttggccttga tgccgctggc cgaggtggtg atgccatcaa cgccggtgcc ggtcaggatc
    18781 gtcatgccct gcttggccag cgatttctcg aggaaggtcg agacgtcctt gtcctccacc
    18841 ggcacgatcc ggtcgagcat ttccacaacc gtgaccttgg cgcccagatc gttgtagaag
    18901 ctggcaaatt caatgccgat cgcgcccgag ccgatgacca gcagcttggt cggcatttcc
    18961 ttgggcgtca tcgcatggcg ataggtccag atgcgctcgc catcggcctt ggcaaagggc
    19021 agatcgcggg cgcgcgcgcc ggtcgccacg atgatgtgct tggcggtaag cgtttcggtg
    19081 cccttctcgc cggtcacttc cagcgtgccg ggcttgagga tcttgcccac gcccatgtgg
    19141 acggccacct tgttcttctt catcaggtgg gtcacgccct ggttcagctg cttggcaacg
    19201 ccgcggctgc gcgccacaac cgccgccaga tcggccgaaa caccggtggc cgacaggcca
    19261 taggccttgg catgctgcat ctggtgaaac acctcggccg agcgcagcag cgccttggtc
    19321 gggatgcagc cccagttgag acagatgccg cccagattct cgcgctcgac aatcgctgtc
    19381 ttcagcccca gctgggctgc gcgaatggcc gccacatagc ccccgggacc cgaacccaga
    19441 acgatcacat catattgttc agccacgaaa attactcctg aacgatcgga cgcggacgcc
    19501 cgctgtcatc aaccgcgaca aaggtgaaca ccgcttctgt caccttgatc gcctccgtct
    19561 ggaagcgatc ccggcgccag gcctcgatgg cgatcgtcat cgacgtgcgc cccaccttgc
    19621 gcaactctcc ataaacagag acttcatcgc ccaccttgac aggcttcaga aactgcacgc
    19681 catccatggc gatggtgacc gcccgcccgc gcgaatggcg cgaggccatc agccccgcgc
    19741 ccatgtccat caggctcagc agccagccac caaaaatgtc gccataggca ttggcgtcgg
    19801 ccggcatggc cgtcacccgg atcatcggct ccatcgccgg actgagattc aagggttcca
    19861 ttgccggcat ctccccagcc tcaggccagc agcgccagcg ggttttccac cagctcgcgg
    19921 aaggccgcca tcagctgcgc gccatcggcg ccatcgatgg cgcggtggtc gaagctgccg
    19981 gtggcgctca tcgcggtggc cacgcccagg ctgccgtctt ccagcaccca ggggcgcttc
    20041 tcgccggcgc cgatcgccat gatcatgccc tgcggcgggt tgatcaccgc ctcgaactgg
    20101 cggatgccca tcatgcccat gttgctgagg ctggcggtgc cgcccacata ttcgctgggg
    20161 gccagcttgc cggccttggc gcgggtggcc agatcggcca tgtcggtgct gatcctggcc
    20221 aggcccttgc cggccgcatc ggtgatgatc ggcgtgatca ggccattggg gatgctcacc
    20281 gccacgctca catcggcgcg ggcatactgg cgcagcgtgt cgccggcaaa ggccacgttg
    20341 cactggggca cctgcgtcag cgccacgccc agcgccttga tcagcatgtc gttcaccgac
    20401 agcttcacgc cgcgggcggc cagcgcgcca ttcagctcgc cgcgcagctt cagcagcgca
    20461 tccagcttga tatccaccgt gaggtagatg tgcggcacct gctgcttgct ctcggtcagg
    20521 cggcgggcga tcgtcttgcg catgccggaa agcgcgacat cctcatgcgg gatgccgaaa
    20581 tcaggcaggc cagcggacgc tgccaccggg gcgggcgccg ccacaggggc ggccacaggg
    20641 gcagcagcag gcgcagcggc agccggccgg gcgccttcca catccgcctt gacgatgcgg
    20701 ccattggggc cagagccggc gataccggcc agatccacgc ccttctgcgc ggcaatgcgc
    20761 ttggccagcg gcgaggcaac cacgcgctcg ccagcggcca caggcgcctt cacggcgacg
    20821 gccgcggcgg ccacaggtgc tgcggccacc ggggcggcgg caggctttgc ggcaggcgcg
    20881 gcagcggcgg gcaccgcgct ggcgtcctca tcctcgccgg ccagctgggc gatgatggtg
    20941 cccacggcca cgccatcggt gccttctgcc acgtcgatcg agacgatcac gccctcatcc
    21001 acggcctcga attccatcgt cgccttatcg gtttcaattt cggccatgat atcaccggaa
    21061 tgaacctcat caccaacttt aaccagccag cgggcaagct ttccctgctc catggttggc
    21121 gaaagggcgg gcatcttgat cgcgatcggc atggcgatgt gggtatcctc ttgcgggatg
    21181 gaagggaatc ggggccgttc tggccaaaag aagagtgtac ggcaagatgc ttgcgccgaa
    21241 ttcgattgta gctgatagct gcggcgtaat tcttatgggc ctcgggcggg gatcgccccc
    21301 gccggcagcg gccctgcggc aggagtttcg cacatgcgcg tctatctggc gatcctggac
    21361 gaaaccgagg aagcgcgcgc ggcgctgcgg tttgccgccc gccgcgcggc caaaaccggg
    21421 ggcgtcgtgc atattctggc gctggtgccg cgccagccct tcgtggcctt tgccggcgtg
    21481 caggccacca tcgaggaaga agcgcgcgcc cgcgccgaag cgcaggcggc cgttgccgcc
    21541 agttccgtgc tgaccgaggg cggttttccg ccccatatcg aggtgcgcga gggcgaaggc
    21601 atcaaggtcg tgcgcgaata tctggccgcc cacgcagaag tggccgcgct ggtgctgggc
    21661 tcggcgcgcg atggggcgcc ggggcccttg gtcacgcatt tttccagcca tggcatcggc
    21721 gatctgccct gcccgctgat gatcgtgccg ggcggcctgg ccgaggcgga tatcgaccgg
    21781 ctgagctgaa cgctccggca ggtcgatcgc ccgggccaaa gcgcgccggc cctagcgccg
    21841 cttgccctgg tggcggatat tggccggccg gccgcgccgc cccaccagat gcgcgccccg
    21901 ccgcttctgc gcggaaggat cgggcgccgg cgcgccgcgc ctttcgatgc ggccgccgcc
    21961 ttcctccggc tcgaacttca gcgcgcccgt cagcggattg gcctcggcca ggcgcaggcg
    22021 cagcatatcg cccatggcaa agcgcgtgcc ggtgctttcg cccaccagcg cctgggcctt
    22081 ctcgtcatgg gcaaagcgct cttcgcccag caccgagatc ggcaccagcc catcgccgcc
    22141 cagccccatg atcgtggcaa acaggccaaa gcgctgcacg ccagtgatcc ggcactcgaa
    22201 cacctcgccc acgcgggtag acagccaggc cgccacatag cggtccaccg tctcgcgctc
    22261 ggcctccatg gcgcggcgct cggccatgga aatggaatcg caaatcggcg ccagatcggt
    22321 gcgatcgcgc gcggcgagcc cactggtggc aggcaagtgc cccttgggcg ccggctgctc
    22381 cagcccatag ccatcgacca gcgcgcggtg gatcagcaca tccgaatagc gccggatcgg
    22441 cgaggtgaaa tgcgcatagg aacccagcgc caggccaaaa tggccggcat tcctcgggcc
    22501 ataataggcc tgcgtctggc tgcgcagcac cgcctccatg atcagcgcct tctccgcctc
    22561 atcgtcgatc ttgcgcagca tggcgttgaa cagttcgggc gtgatcacct ggcccagcgc
    22621 cagcttgcgc ccgaaagtgg cgagataatc cttcagcgtg atcagcttct cgcgcgtcgg
    22681 cggctcgtgg atgcggtaga cgaccggctg caccttggct tccagcgcct tggccgcggc
    22741 aacgttggcg gcgatcatga aatcttccac cacccggtgc gcatccagcc gctcacgcgg
    22801 ggcgatgttg aggatgcggc cctgatcgtc cagcatcacc cgccgctcgg gcagttccag
    22861 ctccagcgga tcgcgccgct cgcgcgcctt gttaagcgcc ttccacgcct cccacagatg
    22921 cacgaaatgc gcctcggcgc ggccctcgtc gatgcgcgcc tgcacatcct cataggccac
    22981 cacttcggcg atctgcacaa tggcgcgcac aaaacgccag cttttcagcg tgccgccggc
    23041 atcgatctgc aaatggcaga ccatcgccgc acgcggcgcg ccagccttca gcgaacagac
    23101 atcggccgac agcacctcgg gcagcatcgg caccacgcga tcggggaaat agaccgaatt
    23161 gccgcgctga cgcgcctcgc gatcaagcgc gccgccagag cgcacataga acgacacatc
    23221 ggcaatcgcc accaccgcgc gccagccgcc cttgttgtcc tcggcctcgt cgggctcggc
    23281 ccagatggca tcgtcatggt cgcgcgcatc gctcgggtcg atcgccacga tcggcaggtg
    23341 ggtgaggtcc tcgcgctgct cggcgctcag cggctggcgg gcggcttcct cggcctcttc
    23401 cagcacggca ttgctgaaat tccacggaat gccatatttg tggatggcaa tcaggctgaa
    23461 cgccttgggc gccagcggat cgcccagcac cgccgtcacc ttcacgcccg cgcgcggcga
    23521 gcggctgacc ggttccgcca gcaccagctg gccttccttc gcctcgccca gatcggcaat
    23581 gcccggcgca tggcgctcgc gcttgtcgat cggcgagagc cagggcttgc cggccccgtc
    23641 gatctccacc acgcccatca gctgctcggc ccgtgccggc agcttcttca tcggatgggc
    23701 cagccagccg tgttccagct cctcggtgcg ggccagcaca cggtcgccca ccttcagcgc
    23761 gcccacgccc ttcatcaccg ggcccttgcc gccgcgcccg gcgccgcgcg gctccacgat
    23821 gcgcaggcgc gggggcgctg ccgcctcatc cggcgcccag acatcaggca cggcgatggg
    23881 cgctgcatcc tcgatctcca ccacccgcag cacgcacacc cggggcacgc cgcccatgcg
    23941 gtgataggcg gtgcgcttgc cgtcgatcag cccttcctcg gccatgtcct tcaacagggc
    24001 cttcagctgg atcttctcct gccccttgag gccgaaggcg cgggcgatct cgcgcttgcc
    24061 cacttcctcc tcggcattgg cgatgaaatc cagcacctgc tgggcgctgg gcaggccctg
    24121 gggcagcttg cccggcacgg atctggggcc gcgcgccaac tcagtaggcc cgcgcgacat
    24181 agatgcgctc caccgccggc gcgccggtga aggggcacag cccctcggcg ggcgcggcat
    24241 cgagcgcggt gttgcggatg gtgagcttca gcgccttcag ctgctccacc acctgctcca
    24301 gcgcggcgcc cgtcggccgg ctccagttca gctccaccca gccgggatag cgctggtctt
    24361 cggcaaagaa ggtggccagc tcggccaggg tggtgatgcc ggtgcggata ttggcggcat
    24421 ggcgcgcggc tgcctcggca tggaggctgc gctggatatc ttccagcagg gccgctgcct
    24481 cgcccacgaa atcttccttg gcctggccgg tgaagttcac cttggcatcc gcgcgccaca
    24541 gcgcatcgcg gcgcagcacc gacacctggc cattggccgc atcgcgcccg ccgatttcca
    24601 gaatgaccgg cgcgcccttc ttcacccagg cccagcgctt ctgcgtggcc ttgccggcgc
    24661 gcttgtcgag cagcacacgg atcggctctg ccagcgcgga ctggctgacc agcgcccggc
    24721 gcagctcctc gcaataggcc agcagcgctt catcgccttc atcttcgcgc agcatcggca
    24781 ggatgatgat ctggtgcggc gcgatggccg gcggcacgcg caggccgtca tcatcgccat
    24841 gggtcatgat aacgccgccg atcagccggg tggaaacgcc ccagctggtg gtgtgggcca
    24901 gcgcctgcgc gccctccctg tcctgatagc ggatgccggc ggcctcggca aaaccggtgc
    24961 cgaggtaatg gctggtgccg gcctgcagcg ccttgccatc ctgcatcatc gcctcgatcg
    25021 aataggtggc aacagcgccg gggaagcgct cgttctcggg cttttcgccg gccaccaccg
    25081 gcatcgccag cacatcctcg gcaaagctgc gatacatttc cagcgcgcgc agcgtttcgg
    25141 ccgtggcatc cgcgcaggtg gcatgggcgg tgtgcccctc ctgccagagg aactcgctgg
    25201 tgcgcaggaa catgcgcgtg cgcatttccc agcgcaccac attggcccac tggttcacca
    25261 tcagcggcag atcgcgccag ctttgcaccc agcggctcat cgccgcgccg atcaccgtct
    25321 cgctggtggg ccgcacgatc agcggctctt ccagcttcgc ctcggggtcg ggcatcaggc
    25381 cgcccttgcc atcgccgatc agccggtgat gagtgaccac cgccatctcc ttggcaaagc
    25441 cttccacatg gtcggcctcc ttggagaaat agctcagcgg gatgaacagc gggaagtagc
    25501 agttatcaac gccggcatcc ttgatgcgcg catccatgac cttctggatg cgctcccaga
    25561 tgccatagcc ccagggcttg atcaccatgc agccgcgcac gcccgattcc tcggccatct
    25621 cgccttcgga gacgaccgcc tgataccatt gcgcaaaatc ctccgcgcgg gtgacggaaa
    25681 gggcgtggcg gatggccggg gcgttctgag tcttctggtt catgccagcc cgttagcctg
    25741 cctgagcgcc ggtttcaaac cccaccgctc aggccagctg tgcaagaggc gcgcaagggc
    25801 gccttacttg gtgagcgaat cgagcttctt ctgcatctcg agcatctgcg cgcgcagcac
    25861 atcgagatct tccttgcccg cggcaggcgc cgcggtgggc gcggcctccc cgggctgatc
    25921 catccccttg gccggcacgc cgccttccgc gcccggcatg aaagcagcgg ccgcggcctg
    25981 gaacatggcg atgttctgct gggccaagcg agccagcggg ttggcctcca tgctgtcctc
    26041 gaaggccttg cgcagcttca tctggttggc gcggaactgc tccatgctgg cttccagata
    26101 atgcgggatc agcccctgca tcgaattgcc atacatgccg atcagctggc gcaggaagtt
    26161 caccggcagc atctgctcgc cgctggcttc ttcctccatg atgatctggg tgaggatctg
    26221 gtgggtgata tcggtgcccg tcttggcgtc gagcacgcga aaatcgatgc cctcgcgggt
    26281 catgcgggca aggtggtcca gcgtgatgta gctggaggac tgggtgttat acaggcgccg
    26341 gttggcgtat tttttgatga tgaccgaatt gtccttggcg gaagcatttt ctgtcataag
    26401 ggcctctcct ccctgtgtga tacgcgaggc caagcttgca cccaaaaccg gtgcggtgca
    26461 acaaattgct gcgcagcaac aggccttgcg cgcaagatta tgcggcgcgg ctgaagcgcc
    26521 ggcccagcac cgtcagcagc tcgtagggag acaggcccac gcgcgcgctg gcctcggcca
    26581 gatcataggc cacatccagc cagtcgccct cggcgagatc gggcgcggcc gacagatcga
    26641 cgatggtaag gtccatcgac acccgcccca ccaccggcag cacccgcccg cccgacagca
    26701 gcgccccccg ccccgaccag cagcgcagat agccatccgc atagcccagc gagaccaccc
    26761 ccagccgcat cggcacaggc gcggtgaaag tggcattata gcccaccttt gcccccgccg
    26821 gcacatcgcg cagctgcagc agggcggcct gcgggcgggc cacctggcgg atgcgcccgg
    26881 ccagctccgg ccgcgccagc ccgccataca gcgccagccc cggccgggtg agatcgccgt
    26941 gataatccgc ccccagcaga atgcccgcac tgttggcaag gctcgcccgg cgatgcggca
    27001 caagcccgcg cgcctgctgc cacagcgcca gctgggcagc attctgcggc gtctcctcct
    27061 cggccgaggc gaggtgggag agcagcacat ccaccgccag cgcctgcaca ccggcatcgc
    27121 ccagcgcttc tgccggcagg cccagccggc tcatgccggt gtccaccatc acatggcagg
    27181 cgccgccgcc cgccgccgcc cagccccgca cctgcgccat ggtgttcagc accggccgca
    27241 cgcctgtggc gcgggcaaag gcgatatcct gcgcccctgc cggcccatgc agcacggcga
    27301 tctgctctgc cggcaccaca tccagcaatg cggccgcttc ctgccagtgg gccacgaaga
    27361 aatcgcggca tccggccgcg gccagcaccc gcgtggcgcg caaggcgccc agcccatagg
    27421 catccgcctt caccgccgcc ccggcctgcg cagagcccga cagcgcatcc atcaaccgcc
    27481 agttggcggc cagcgcttcg gcatccagca gcagccgcaa gggggcggca ggcgcctcaa
    27541 ccatcggcaa attcctgctt gcggccgccc ttcagccccc acagcgccac catcagcgcc
    27601 agcgccacca cgccccaggt ataccacaga ccggcatagg gattgccgct gcgtgccacg
    27661 atatagctgg cgatcagcgg caggaagccg ccgaaatagc ccgtgcccag gtgatagggg
    27721 atcgacatgg aggaatagcg gatgcgcggc ggaaacatct ccgacagcag cgcggcctgc
    27781 ggcccgaagg ccagcgccgc caagccaacc agcccgacga tcagcgcgat aatgcccaca
    27841 gaagcgccgg gccccggcgt caccttggca aagctgtagc cttctgcgcc cagccagccc
    27901 tgcagcatcc tggcccgcgc ggccttctgc gcgaagggat aggcggcaat ggcctgcgcc
    27961 ttgccgccca ccgtcaggct cagcacggcg gcatcgctgc gggtataggt cacgcccagc
    28021 gccgtcaggt cggccagcag cttgccgcaa tcgctcggct gctcgctggc aaaggggctg
    28081 taatcgcagg ccgggccgct caccgccacc ggcgcccgcc gggccgcttc tgccagcccc
    28141 ggattggcca gcgcgccaat gccatggaac agcggaaaga cgaacagcag cgtcagcaca
    28201 taggaagcga tgatcggcag cttgcgcccc accctgtccg acagcgcgcc ggccgcgata
    28261 tagagcaccg cccccagcga gccgcccacg cccaccagca gctcggccgc tgtatcctcc
    28321 agatgcatcg cccccttgag ataggagagc acgctgaaca tgccggtata ccagatcacc
    28381 gtcatccccg ccgccacgcc aaacagcgcc acgacaatcc gcttcacatt gccgggaaag
    28441 gtgaaactct cgacaaacgg gttgccggcc agctcgccct cctcgcgcat ggcctgaaac
    28501 accgggcttt ccgaaagctt caggcgcatc cacatcgaga ccgccagcag cagcaggctg
    28561 atcaggaacg gcacgcgcca cgcccagccg gcccattgca ccggcgcaaa agccgccttg
    28621 cagctgagca ccaccaccag gctgagcaga aagccgccaa ccacgctggc ctggatgaag
    28681 ctggtgaaga agccgcgccg ctctgcccgc gcatgttccg acacatagat cgccgcgccg
    28741 ccatattcgc cgcccagcgc cagcccctgc aacacccgaa ggccgatgac gatcaacggc
    28801 gcggccatgc cgatgctggc cgccggcggc accagcccaa cgccggccgt ggccacgccc
    28861 atcagcacca cggttaccag aaacgtgtat ttgcgcccca gccgatcgcc cagaaagccg
    28921 aacagcaccg cccccagcgg ccggaagcca aagcccacgg caaagcccgc ccacaccagc
    28981 agcagctgca gcgtttcatt gccggcgggg aagaaggtct tgccgataat cccggccagc
    29041 gtgccataga tgaagaaatc ataccattcg aacaccgtgc ccatgctgct ggcggcgatc
    29101 accagccgca tgtcggcggc actgggctga atatccggat ggcgtgcagg ctcggtcatt
    29161 ggcgcaatct ccccctctcg tcggccatcg cg
//
LOCUS       DASRYE010000021         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_107233,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000021 DASRYE010000000
VERSION     DASRYE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107233"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..2523
                     /locus_tag="VFF98_01200"
     CDS             295..2523
                     /locus_tag="VFF98_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62271.1"
                     /translation="MGAAPAARAEGEPKAEIPANGEIVVTAQRRAQSVMEVPLAISAI
                     AGAGLASKGIANSVNLAQAVPNLQVSTNFGNAQPNFSMRGVSVANEYNSNQVSPVGVY
                     MDDVYLAARSAHGMGLFDLDRAEVLRGPQGTLFGRNTTGGAINFITRGPKLQGTNGYL
                     DLGYGNFNTFTAQGALETTADNDQLGVRAAVNYVKGDGQFHNVHPGALDPGSQDTLQG
                     RLSLRYRPAGSGLDIRIRAYAGRDNPTSSAVFGPASARQGLDFFSVNEGNIGHFRTSA
                     WGVMANVAYEIHPGLTVTSITSYDGGRLAMQAQSDGTPEDGAYAASAYFPGLYINWHS
                     KFRQFTEETRVNYEHGRLKLVGGAFYGWDRTITDNDFNIGVVPVSATTYLTPGGFFQH
                     YVQNRYSVAAFAQADYEILPRLTLTLGLRETWDKSHYDDGYAYLYAGGALGGTAEVPL
                     ASTVPCYNAAGCAYDPNARYALAEANSALTGRAALSYKFEGGLLAYASYNRGYRSGAF
                     NGGSYTSQSGINYVSPERVNAYELGLKGHLLGRRLTFSAAAFYYDYINQQLQNTLPGP
                     VAILVNAPKSQIYGAEAEVTWRAAPTLSFNGSVGLLHAKYKELTLQGVNLAGQDLPFA
                     PRFTGQIGMDWALYRHGADELTLSPNLAYTGHVYFTPFNLLNASAAQDNAQLQQGGYA
                     KVNAQLVWKHAPFQLRLWVNNAFNARVLAYGLDLRGAGFPYNFLVPANPRSFGLSGRV
                     SF"
     gene            2523..3743
                     /locus_tag="VFF98_01205"
     CDS             2523..3743
                     /locus_tag="VFF98_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU62272.1"
                     /translation="MAADLKNPDLYVEAVPHALFAQLRREEGVYWNAEADGPGFWAVL
                     RYADIVEVSRQPGLYSSAHAHGGHRIFNENEVGLTGAGDSAIGIPFISIDPPVHTQYR
                     KLMMPAVSPARLAGIEARILERCQGLIEAIAPEGPVDLVQALCAPLPLMTLCELFDLP
                     PEVDWRDLYHWTNAFVGEDDPEFRQSPAQMAALLTGFFALTGQLFEARRGRPGADLAT
                     LLANAEIDGAPVAYRDFLANMVLALVGANETTRNSLSHTVVNFAANPDQWDLLRQTPD
                     LVQTAVREMVRHASPVMHMRRTATQDTELGGRRIARGDKVVLWYTAANRDEQVFAEPD
                     RFDITRGNIQHLGFGSGQHVCVGSRLAEMQLRVAFRLLAERVGRFELVGRPRRFRSNF
                     INGLKNLDVKLHPA"
     gene            3770..4654
                     /locus_tag="VFF98_01210"
     CDS             3770..4654
                     /locus_tag="VFF98_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZU62273.1"
                     /translation="MQEALRFNPAGTSEAIISDAIGRGPDMQLGSKGDGWHLCRWRQF
                     VGSYTVPALPEPVYTMHIAGQQHNRTWLDGGWSEYASMPGCATIVPAGQQTGWLIDGE
                     LDVVTLSVSSDQLQKAPAADQFRQMRFAFSDPLGAALTRQVLSELYAPQCAEREVYIG
                     ALVNALKAHILRGANSVTAQEIPTSAFSAYRIHHVMNTILQQPGAQHSLEEMSAVAGL
                     TPSHFCRVFRRATGLSPHQYVMKTRLDKAQQMLHQTDMSIALIAESLGFTSQSHFTRA
                     FRQFSGEAPSDFRKRTRN"
     gene            4659..>5113
                     /locus_tag="VFF98_01215"
     CDS             4659..>5113
                     /locus_tag="VFF98_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU62274.1"
                     /translation="MTMQAGFAHLQLPDMQLRSGEELLPETGRYGLEDPCTGAFFADC
                     PVAGEAAVAAAVAAARRALADPAWRDLAPLARERLLHGLADAMEADLPRLAALEALDC
                     GKPVAIAEAVDVPAAIAWLRAYAGWPGKLMGSAGQLAVTPGAHHVYSRR"
BASE COUNT          836 a         1683 c         1713 g          881 t
ORIGIN      
        1 cggcggcgtg gtggcgcgga tgtagcaagt gggcctctcc catatggtct gcgggttggc
       61 cccgctgctg ccgggggaga aacagtgcgg gggcccgtgg cgcaatggcg gcgcaggaaa
      121 tgtcaagaat cgcagcaggc agggctaaag ccatcgcccg gcgccccggc tacacaggcc
      181 tcaccatgaa gcagaccgct ggcacgagcc ggcggcggat gggaggacag gatgcgttgg
      241 ctggctgaac ggggcttggg tgtgtgtgcg tcggcagtgg cgctgggggt ggccatgggg
      301 gctgccccgg ccgcgcgcgc cgaaggggag cccaaagcgg aaatccccgc caatggcgaa
      361 atcgtggtaa cggcccagcg ccgcgcgcaa tcggtgatgg aggtgccgct ggcgatctcg
      421 gcgattgccg gcgcggggct tgccagcaag ggcatcgcca attcggtgaa cctggcgcag
      481 gcggtgccca acctgcaggt cagcaccaat ttcggcaatg cccagcccaa tttctcgatg
      541 cgcggcgtgt cggtggccaa tgagtataat tcgaaccagg tctcgccggt gggcgtctat
      601 atggacgatg tctatctggc ggcgcgttcg gcccatggca tgggcctgtt cgatctggat
      661 cgcgccgaag tgctgcgcgg gccccagggc acgctgtttg gccgcaacac cactggcggc
      721 gctatcaatt tcatcacgcg cgggcccaaa ttgcagggca ccaacggcta tctcgatctg
      781 ggctatggca atttcaacac tttcaccgcg caaggggcgc tggaaaccac ggccgacaac
      841 gaccagctgg gcgtgcgcgc ggcggtgaat tatgtgaagg gcgacgggca gttccacaac
      901 gtccaccccg gcgcgctcga tccgggcagc caggatacgc tgcagggccg cctgtcgctg
      961 cgctatcggc cggcgggcag cgggctggat atccgcatcc gcgcctatgc cgggcgcgac
     1021 aatcccacct catcggcggt gttcgggccg gcctcggcgc gccaggggct ggatttcttc
     1081 agcgtcaatg aaggcaatat cggccatttc cgcaccagcg cctggggggt gatggccaat
     1141 gtcgcctatg agatccaccc cggcctgacg gtcacctcga tcacttccta tgatggcggg
     1201 cggctggcca tgcaggccca gtcggatggc acgcccgagg acggcgccta tgccgccagc
     1261 gcctatttcc ccggcctcta catcaactgg cattcgaaat tccgccagtt caccgaggaa
     1321 acccgcgtca actacgagca cgggcggctg aagctggtgg gcggcgcctt ctacggctgg
     1381 gatcgcacca tcaccgacaa tgacttcaac atcggcgtgg tgccggtttc tgccaccacc
     1441 tatctgacgc cgggcggctt cttccagcat tatgtgcaga accgctactc cgtggccgcc
     1501 tttgcgcaag ccgattacga gatcctgccg cgcctgacgc tgacgctggg cctgcgcgag
     1561 acatgggaca aatcgcatta cgacgatggc tatgcctatc tctatgccgg cggcgcgctg
     1621 gggggcacgg ccgaagtgcc gctggcttcc accgtgccgt gctacaatgc ggccggatgc
     1681 gcctatgatc ccaatgcgcg ctatgcgctg gcggaggcca attcggcgct gaccgggcgc
     1741 gcggcgctca gctacaagtt cgagggcggg ctgctggcct atgccagcta taatcgcggc
     1801 tatcgctcgg gcgctttcaa cggcggcagc tacacctcgc aaagcggcat caactatgtc
     1861 tcgcccgaaa gggtgaatgc ctatgagctg gggctgaagg gccatctgct gggccgccgg
     1921 ctcaccttct cggccgccgc cttctactat gattacatca accagcagct gcagaacacg
     1981 ctgccgggcc cggttgccat cctcgtcaac gcgcccaaat cccagatcta tggcgccgag
     2041 gcggaagtga cctggcgggc agcgccgacg ctgtccttca acggatcggt gggtctgctg
     2101 catgccaaat acaaggagct gaccttgcag ggggtgaacc tggccggcca ggacctgccc
     2161 tttgccccgc gcttcaccgg ccagatcggc atggactggg cgctctatcg ccatggcgcg
     2221 gatgagctga cgctgtcgcc caacctggcc tatacgggcc atgtctattt caccccgttc
     2281 aacctgctca acgccagcgc cgcgcaggat aatgcccagc tgcaacaggg cggctatgcc
     2341 aaggtgaacg cccagctggt gtggaagcat gcccccttcc agctgcgcct gtgggtgaac
     2401 aatgccttca acgcgcgggt gctggcctat gggctggatc tgcgcggggc gggcttcccc
     2461 tacaacttcc tggtgccggc caatccgcgc agtttcgggc tcagcggcag ggtcagcttc
     2521 tgatggccgc cgatctcaag aaccccgacc tctatgtcga ggcggtgccc catgccctgt
     2581 ttgcgcaact gcgccgcgag gagggcgtct actggaacgc ggaggctgat gggcccggct
     2641 tctgggcggt gctgcgctat gccgatatcg tcgaggtgtc gcgccagccg gggctctatt
     2701 cctcggccca tgcccatggc ggccaccgca tcttcaacga gaacgaggtg gggctgacag
     2761 gcgccggcga cagcgccatc ggcatcccct tcatctcgat cgatccgccg gtgcacacgc
     2821 aatatcgcaa gctgatgatg ccggccgtct cgcccgcccg gctggccggc atcgaggcgc
     2881 gcatcctgga gcgctgccag ggcctgatcg aggcgattgc gccggaaggg ccggttgatc
     2941 tggtgcaggc cctgtgcgcg ccgctgccgc tgatgacgct gtgcgaattg ttcgacctgc
     3001 cgcccgaggt ggactggcgc gacctgtacc actggaccaa cgcctttgtg ggcgaggacg
     3061 accccgaatt ccgccagagc ccggcgcaga tggccgcgct gctcaccggc ttcttcgcgc
     3121 tcaccggcca gctgttcgag gcgcggcggg ggcggccggg ggcggatctg gccaccctgc
     3181 tggccaatgc cgagatcgat ggcgcgccgg tggcctaccg cgattttctc gccaacatgg
     3241 tgctggcgct ggtgggcgcc aatgaaacca cgcgcaattc gctgagccac acggtggtga
     3301 atttcgccgc caaccccgac cagtgggatc tgctgcgcca gacgcccgat ctggtgcaga
     3361 ccgcggtgcg cgaaatggtg cgccatgcca gcccggtgat gcacatgcgc cgcaccgcca
     3421 cgcaggacac cgaactgggc ggccggcgca tcgcccgggg cgacaaggtg gtgctgtggt
     3481 ataccgccgc caatcgcgac gagcaggtgt ttgccgagcc ggatcgcttt gacatcaccc
     3541 gcggaaacat ccagcatctg ggcttcggca gcgggcagca tgtgtgtgtg ggctctcgcc
     3601 tggccgaaat gcagttgcgg gtggcgttcc ggctgctggc agagcgggtg ggccgcttcg
     3661 agctggtcgg ccggccgcgc cggttccggt ccaatttcat caacgggctc aagaatctcg
     3721 acgtgaaatt gcaccccgca tgaagatcaa cgcccttggg agagggatta tgcaagaggc
     3781 actccggttc aacccggccg gcaccagcga ggcgatcatt tccgatgcca tcgggcgtgg
     3841 gccggacatg cagctgggct ccaaaggcga tggctggcac ctgtgccgct ggcgccagtt
     3901 cgtcggctcc tacacggtgc cggcgctgcc agagcccgtt tacacgatgc atatcgccgg
     3961 ccagcagcac aaccgcacct ggctggatgg cgggtggagc gaatatgcct ccatgcccgg
     4021 ctgcgccacc atcgtgccgg ccggccagca gaccggctgg ctgatcgatg gcgagctgga
     4081 tgtggtgacg ctgtcggtct cgtccgacca gctgcagaag gcgcccgcgg ccgatcagtt
     4141 ccgccagatg cgctttgcct tttccgatcc gctgggcgcg gcgctcaccc ggcaggttct
     4201 ctcagagctc tatgccccgc aatgcgccga gcgcgaggtc tatatcggcg cgctggtcaa
     4261 tgcgctgaag gcgcatatcc tgcgcggcgc caattcggtc acggcgcagg aaattcccac
     4321 ctcggccttc tcggcctatc gcatccatca tgtgatgaac accatcctgc agcagcccgg
     4381 cgcgcagcat tcgctggagg agatgtcggc ggtggcaggg ctcacgccct cgcatttctg
     4441 ccgggtgttc cgccgcgcca ccgggctcag cccgcaccag tatgtgatga agacccggct
     4501 cgacaaggcc cagcagatgc ttcaccagac cgacatgtcg atcgcgctga tcgccgaaag
     4561 cctgggcttt accagccaga gccatttcac ccgcgccttc cgccagttct ccggcgaggc
     4621 gcccagcgat tttcgcaagc gaaccaggaa ctaggacaat gaccatgcag gctggctttg
     4681 cccatctgca actgcccgac atgcagttgc ggagcgggga ggaattgctg ccggaaacgg
     4741 gccgctatgg cctggaagac ccatgcaccg gcgcgttttt cgccgattgc ccggtggccg
     4801 gcgaggcggc ggtggcagcg gcggtggcgg ccgcgcggcg ggcgctggcc gatcctgcct
     4861 ggcgtgatct ggccccgctg gcgcgcgagc ggctgttgca tggcctggcc gatgcgatgg
     4921 aggccgacct gccgcgcctt gccgcgctcg aggcgctcga ttgcggcaag ccggtggcga
     4981 ttgccgaggc ggtggacgtg cccgccgcca ttgcctggct gcgtgcctat gccggctggc
     5041 cgggcaagct gatgggcagc gccggccagc tggcggtcac gcccggcgcc caccacgtct
     5101 attcccggcg cga
//
LOCUS       DASRYE010000022        24848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_1160,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000022 DASRYE010000000
VERSION     DASRYE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..880)
                     /gene="panC"
                     /locus_tag="VFF98_01220"
     CDS             complement(32..880)
                     /gene="panC"
                     /locus_tag="VFF98_01220"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZU62275.1"
                     /translation="MQIIEELHPLRRALEALRAEGPVALVPTMGALHEGHLTLVRAAR
                     ARGAQVVASIFVNPRQFAAGEDLEAYPRQLARDAALLEAEGVAVLWAPTVSAMYPAGY
                     ATNVSVAGVSEGLCGGTRPGHFDGVATVVLKLFNQVAPDIALFGEKDWQQLAVIRRMA
                     RDLDLARPAVENIIGVATVREADGLARSSRNAYLSATERQQAACLPAAMHKAIAALQA
                     GTPVPEALAALEADLLAGGFSAVDYAELREADSLAPLATVGTVPARLLVAARIGKARL
                     IDNMAV"
     gene            1005..1928
                     /locus_tag="VFF98_01225"
     CDS             1005..1928
                     /locus_tag="VFF98_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HZU62276.1"
                     /translation="MSDASPPSPGPHRIVFANEKGGTGKSTTAVHVAIALAYLGGRVA
                     AIDLDPRQRTLHRYFENRAETLRRRQISLPMISFGVFNGSTTEELDGLSAAMGAGHDY
                     LIYDTPGRDDVFARHVATQADTLVTPLNDSFVDFDLIGQVDSESFRVRRLSFYAELIW
                     ETRKKRALSTLADNRREMDWVVVRNRTQHIEARNMRRIDGALAELARRVGFRIAQGLS
                     ERVIYRELFPSGLTLLDKGYLGDLGTSHLVARQELRALLSGLSLPLPPFAPAPAAMAP
                     PMGQAPMEQPAMEQAPMEQAGSEPAPVQPYY"
     gene            1950..2261
                     /locus_tag="VFF98_01230"
     CDS             1950..2261
                     /locus_tag="VFF98_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZU62277.1"
                     /translation="MSKLIWLAAIGCLVFKWQTGRWPWQARGALPGRGRGAEQALAHA
                     RALLGLGAGASHGEIIEAHKRLLMIVHPDRGGTSEQVHEATAARDLLLADLARRSAAG
                     Q"
     gene            2266..3735
                     /locus_tag="VFF98_01235"
     CDS             2266..3735
                     /locus_tag="VFF98_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZU62278.1"
                     /translation="MTYRLHPSILREYDIRGICGETLWPADAFVLGWAFARVVAEAGG
                     RRVAVGRDGRLSSPVLEAALAAGLRAGGAAVLPVGQGPTPLLYFAERSTEQVEGGIQV
                     TGSHNPPDHNGFKIVLQGAPFFGADLLRLGQIAEGFDPAVLPEGFGAVWHGLAGGAGV
                     DPILGDADGAGEGGEIFRRYIDALTAALEEAPAGPLAALRVGWDAGNGAAGPAIEALC
                     ARLPGEHHLLYTRVDGHFPHHHPDPTVEENLADLRSLVLSERLDFGVAFDGDGDRLGV
                     IDGHGRVVWGDQLLLLFAADLLESHPGARILADVKTSQGVFDGIAALGGQPLMCETGH
                     SWVKSRMKQAGALLAGEMTGHLFFADRYFGFDDALYAAVRLMAAVARQERSVADMLEA
                     MPVWVNTPELRFAVPAARKAAVVAEVAARLRAEGADVVTIDGLRVRRADGWWLLRASN
                     TQEMLVARAESADAAGLARLLAELDGQLAASGVARAGGH"
     gene            3769..6231
                     /locus_tag="VFF98_01240"
     CDS             3769..6231
                     /locus_tag="VFF98_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HZU62279.1"
                     /translation="MAATDPLPPELAGWFAGRGWRVRGHQLAMLAEADAGRHALLMAD
                     TGAGKTLAGFLPTLAAFCPSRQGAAPVPEGLHTLYVSPLKALAQDVQRGLARPVADLG
                     LPLRIETRSGDTPSDRKARQRARPPHVLLTTPESLSLLLTCPESAEMFASLKRVVVDE
                     LHAFAAGKRGDLLALALARLQVLAPQMRRAGLSATLADVAAARAWLAPGDEAGDVALV
                     TGEAGAAPVVEILEPRGQRVPWAGHAAAWAVPQIYEAIGRHRTTLVFTNTRFLAEYIF
                     QRLWDANADHLPIGLHHGSLSREARARVEAAMAEGRLRALVCTSSLDLGVDWGDVDLV
                     VQMGAPKGSSRLLQRIGRANHRLDQPSKALLVPGNRFEYLEALAARDAVAEGQRDGEG
                     FRPGALDVLAQHVMGLACAGPFDEAALLAEVRSAAPYHWVEEGVWQRVLHFVATGGFA
                     LKAYDRFRRLVRSAHGAWRLAHPEMAARHRLNAGIIVDAEMVEVRFGNGRSLGRVEEG
                     FAASLAPGDTFRFAGLDLELMRLGHAEMQVRAAKRAGAIPSYMGARIPISSHLAARVR
                     AMLARPALWPALPDEVREWLEMQAHVARLPGEEGLLVESFPHAGRHHAVFYTFEGWPA
                     NQSLGMLITRRMEAQGLAPLGFVASDYALAVWGLRPIEAPGALLSADILAEEFLPWVR
                     ESHLLRRAFREVAVISGLVERQQPGQRKSGRQVRFSTDLIYDVLQKYEPDHLLIEAAW
                     ADARQRLTDVARVAEVLERAGRAIEHVPLARVSPLAVPVLTMIGREGLPAGSGEEELL
                     FEAEALAALAMGDMEACAAAPQ"
     gene            complement(6225..7007)
                     /gene="hisN"
                     /locus_tag="VFF98_01245"
     CDS             complement(6225..7007)
                     /gene="hisN"
                     /locus_tag="VFF98_01245"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HZU62280.1"
                     /translation="MKLDADIALAMRLADAAGAAIRPHFRAGLSSERKDDASPVTLAD
                     RAAEEAMRRLLKAEAPRDGIIGEEFGHEGAGATRQWVLDPIDGTAGFLAGRPIFGTLI
                     ALVVDGWPVLGIIDQPILNERWVGASGYQTLLNGKPAHTRACRQLADANIATTGPHYF
                     DEHEGAHFMALAAKTDHRRMVMGGDCYNYAMLASGEVDIICEAGLKLHDWAALVPVVE
                     GAGGTMADWNGDPLHMGSDGHVLALGDPARLEEVVEALACHH"
     gene            complement(7111..8046)
                     /locus_tag="VFF98_01250"
     CDS             complement(7111..8046)
                     /locus_tag="VFF98_01250"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZU62281.1"
                     /translation="MKIMAGNSNLPLARAIAGYLELPLTDAAVRRFADEEVFVEIHEN
                     VRGEDVFVVQSTSYPTNDNLMELLICIDALRRASAKRITAVVPYFGYARQDRKPGPRT
                     PISAKLVANLITTAGADRVLAVDLHAGQIQGFFDIPTDNLFAAPTMAADIQARYGDKD
                     LMVISPDVGGVVRARALAKRLNNAPLAIVDKRRDKPGVSEVMNIIGDVKGRTCIMIDD
                     IIDSGGTLCNAAQALIDAGAASVTAYISHGVLSGAAVSRVSNSALKELVITDTILATE
                     ATQQSDKIRVLTIAPLIGEAIRRIADESSVSSLFD"
     gene            complement(8248..9222)
                     /gene="glpX"
                     /locus_tag="VFF98_01255"
     CDS             complement(8248..9222)
                     /gene="glpX"
                     /locus_tag="VFF98_01255"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZU62282.1"
                     /translation="MTATPASKVLDRVLVLEMVRVTEAAAIASAKLIGRGDEKAADAA
                     AVEAMRAKLNDLYMDGTVVIGEGERDEAPMLYIGEKVGNAIGKGPKIDIALDPLEGTT
                     ICAKAGPNSLAVLAIAEEGGLLNAPDVYMDKLAVGPGYPENIIDLDKSPTENVQAVAA
                     AKGVQPEDIVVCVLDRPRHADLIAELRAIGCGVYLIGDGDVAGVIATTNEETNIDLYM
                     GQGGAPEGVLAAAALRCVGGQFKGRLVFRNEDERLRAKKWGIEDLNKIYDLTELAKGD
                     CIFAATGVTDGSLLKGVKKLKNGRMTTDSVVMRASSGTVRWVKGDRRQ"
     gene            complement(9402..10760)
                     /locus_tag="VFF98_01260"
     CDS             complement(9402..10760)
                     /locus_tag="VFF98_01260"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZU62283.1"
                     /translation="MADSSSPAARPPLRIALAGLGTVGAGVIRLIEANAALIARRAGR
                     PVQVVAVSARERGKDRGVDLSPYAWEDDMTAMAARDDVDVVVELVGGSDGPALTLARN
                     AIRHGKALVTANKAMIAHHGMELAGAAEQAGVALRFEAAVAGGIPVIKGLREGVAANR
                     VERIYGILNGTCNYILSTMEDTGRDFAEVLAEAQAKGYAEADPTFDIEGTDAAHKLSI
                     LSAIAFGARIDFASVDTSGISRILAADIAEANALGYVIRLIGMAESGAESGAESGTGG
                     LFQRVQPHLVPFDNLLAHVDGATNAVVVEGNFSGKLLFQGAGAGDGPTASAVVADIID
                     IARGMDTGFEADMSFSVPVTQLEPLAPANSGARAGCAYIRFLVEDRPGVLAEITAAMR
                     DAGVSIASFSQRGRAEAGGQVLVAMVTHEGPESAVTRALALLEGSASLAGAPLVMQIL
                     GA"
     gene            complement(10863..11705)
                     /locus_tag="VFF98_01265"
     CDS             complement(10863..11705)
                     /locus_tag="VFF98_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62284.1"
                     /translation="MAGPVWCSAAFAAAILGGAVPFARAAPPPPAPAPHAYADIVDLA
                     DSARLVMQAEVRGQATVEPARAPGVKPGWARIYVEARPAVILRGDVPLAESVHYLADV
                     PLDAKGKVPSLKKQQVLLFAHTLARGAEDLQLVAADAQWLADPALVDRVHKAIGDLFA
                     PDAAPPVTAITQALYEPGTLAGEGETQLFLATAKGEPASISVLHQPDQPVHWSVSFSE
                     VVNPDAPPPAHDTLAWYRLACFLPARLPDGINISATPDARLQAERDYRLVLAELGPCG
                     RLRD"
     gene            12022..12672
                     /locus_tag="VFF98_01270"
     CDS             12022..12672
                     /locus_tag="VFF98_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU62285.1"
                     /translation="MAYADQQMSGSRVTALIIVALIHVAVGYALITGLAYSAAKQIIK
                     KVTTVDIKEDKPKPPPPPPPPKQDAPPPPIVAPPPPVNVSVAPPPVQTVSVAPPPAPP
                     VLAPPAPVAPPPPKVAAQRAVPRGNPGDWVTPEDYPSRPLREGVQGVTSFRVTIGPDG
                     RVTGCEITGSSGNDELDKATCTYFARRARFKPAVGSDGQPMTDSYANRVKWVIPKD"
     gene            12759..13508
                     /locus_tag="VFF98_01275"
     CDS             12759..13508
                     /locus_tag="VFF98_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZU62286.1"
                     /translation="MLTEILAAGANSAPQNKFGFMEAMQQGGALSWSIVTILAIFSFG
                     SFFILFTKWADQSKILKQYGNLNAFWKAPTLQEGLAKLDKRSAFRDLVEAGLAAEEQH
                     SKLVDALEAHDWLHGSLARAENSITSYLSRGLPFLATVGSTAPFVGLFGTVVGIYRAL
                     IAIGIAGSASIDKVAGPVGEALIMTAIGLLVAVPAVLAYNFLMSRNKRINEVLSAFSN
                     DVLANINSKGAIKPAVAAAKAAPVAAKPAGK"
     gene            13644..14177
                     /locus_tag="VFF98_01280"
     CDS             13644..14177
                     /locus_tag="VFF98_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU62287.1"
                     /translation="MAMSVGGGGEDKPMSDINTTPLVDVMLVLLIIFLIAVPVAIQTI
                     QKLKIPVMVSQESKDKVENLLLTVATTDEAGHSAGDPGYTGSSRGGECRIYFNNITPV
                     TSDELRDKAYKRLDAIVKREGGAEALRAEPDKVPQVHIRADVNAPWRCVAGAIYNVQV
                     SGYPTVGFISTPVDPNG"
     gene            14208..14666
                     /locus_tag="VFF98_01285"
     CDS             14208..14666
                     /locus_tag="VFF98_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZU62288.1"
                     /translation="MAMSGGKDDGEPMNEMNTTPLIDVMLVLLIMFIITIPVATHAVN
                     IDLPSPSPTPPDIKVEPVKNKVNVTADGKILWDSNPVDQGQLLTLLQQSTQMTPEPEL
                     QFQPEAQAGYDLTAHVLNIIKGSGVTKFGFVGNEQYASFSKAANADKAGE"
     gene            complement(14723..15007)
                     /locus_tag="VFF98_01290"
     CDS             complement(14723..15007)
                     /locus_tag="VFF98_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZU62289.1"
                     /translation="MAIVQTYRLAARADSAAALAAALAELAAALAAAPGTQGTRILRR
                     ADAPAHFLFLEFWDSEEARKAAAPLLPKAAMAALMAALEGKPEVATYADA"
     gene            15073..15534
                     /locus_tag="VFF98_01295"
     CDS             15073..15534
                     /locus_tag="VFF98_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZU62290.1"
                     /translation="MAAALPLRFFFYGTLMGGHGNAGAQVVAAHMRLLGAARAPGRLV
                     GLEEGGGWYPALLPDAGGGQVAGRLYEALPGFGAAQLAAIDRYELCFPEDPGVSEYCR
                     ALVPVAWAGGQGEAQAYLYARALPPGALPIAGGDFARWLADTGRRAFGAAG"
     gene            15686..15889
                     /gene="rpmI"
                     /locus_tag="VFF98_01300"
     CDS             15686..15889
                     /gene="rpmI"
                     /locus_tag="VFF98_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZU62291.1"
                     /translation="MPKLKTKSGVKKRFKLTATGKVKHGVAGKRHRLISHNGKYIRQN
                     RGTDVISDADAKVIKKWAPYGLN"
     gene            15902..16264
                     /gene="rplT"
                     /locus_tag="VFF98_01305"
     CDS             15902..16264
                     /gene="rplT"
                     /locus_tag="VFF98_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZU62292.1"
                     /translation="MSRIKRGVTTRAKHKRILDQAKGYRGRRKNTIRVARQAVEKAGQ
                     YAYRDRKIKKRTFRALWIQRINAAVRAEGLTYSQFINGIKLAGIELDRKALADLAMNE
                     GAIFSAVLAQAKAALPAA"
     gene            16616..17356
                     /locus_tag="VFF98_01310"
     CDS             16616..17356
                     /locus_tag="VFF98_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62293.1"
                     /translation="MDYLGRNQPDLFFSVTGPSTRAVRFTVGSCRIWGIGMPPLGWAR
                     FVGLPAAGTADRVLNGQHEPALARFLPLAQGLYGEERAVERELACLTDFFLALDAGLP
                     PVPDAERIVAVHAALVDHEVSSVAQLVERVGFSHSTLERLCHRAFGFAPKQLLRRQRF
                     IRSLAQYMLDPSLKWIGALDGHYHDQAQFVREFRAFMGMSPRQYAALDLPVLRAATQA
                     RQRLAGAPMQALTGPVSPEPIALGRAGS"
     gene            17443..18531
                     /gene="pheS"
                     /locus_tag="VFF98_01315"
     CDS             17443..18531
                     /gene="pheS"
                     /locus_tag="VFF98_01315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZU62294.1"
                     /translation="MGAEALEKIAAAADLDGLETLRIAYLGKQGSVSALLKTLGAMSP
                     DERQAVGPRIHALREGVTAALAERKAALEGAALDARLAAETVDLSLPAPVAPRGSVHP
                     VSQVMDELAEIFADLGFAVASGPEIEDDWHNFTALNMPESHPARAMHDTFYLPDLTAE
                     GREMVLRTHTSPVQIRAMLAAGGNQPLRIIAPGRVYRSDSDATHTPMFHQVEGLVIGK
                     DIHLGHLKWTLETLLKAFFEREDIVLRLRPSYFPFTEPSVEVDTGYSWQDGRRVVGGS
                     GDAPGHSWMELLGSGMVNAHVLRAGGYDPAEWQGFAFGVGVDRLAMLKYGMNDLRAFF
                     DGDQRWLSHYGFSALDVPTLSAGVGVPA"
     gene            18544..20943
                     /gene="pheT"
                     /locus_tag="VFF98_01320"
     CDS             18544..20943
                     /gene="pheT"
                     /locus_tag="VFF98_01320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZU62295.1"
                     /translation="MKFALSWLKACLDTDAGVDEIAARLNAIGLEVEGVEDPAEKLKG
                     FRVARILTADRHPNADKLQVLSVDTGEGAPLQVVCGAPNARAGLVGVLGLPGAVVPAN
                     GMVLKVAAVRSVESNGMMCSLRELELGEGHDGIIELPEDAPVGTDFASYHGADPVFDV
                     AITPNRPDCMGVYGIARDLAAAGLGTLKPIVAPGIAGSFACPVEIRTEDAEGCPAFYG
                     RVIRGVANGESPAWLQAALASAGQRPISALVDITNYVMLTYGRPAHAYDLARLQGAVV
                     ARRAKAGESCVALNGKTYALDETMTVIADEAGVHDIAGIMGGEHSGCTAETRDVLLEI
                     AYFNPERIAATGRKLNLTSDARGRFERGVDPAFLETGLDLLTGLVLEICGGEASDVVR
                     AGSAPVAPRQVAYDPRLAETLGGVAIAAEEQRRILAALGFAVAEDWTVTAPGWRPDVD
                     GAPDIVEEVVRIHGLDNIASVPLPRVDGVARPTATPAQKLERRLRRAAAARGLNEAVT
                     WSFLPMAEAGHFAAGDLWVLSNPISEDMKAMRPSLLPGLLMAARRNLARGAAGLGLFE
                     IGRRYLRGSDGRSDEKLTLAVALVGEKAARGWDARANGGKAQAFDAFDAKGAALALLA
                     EAGAPVDNLQIMGEAGSQFHPGQSATLRLGPKNVLARFGMLHPATLKAFDIEVPVAVA
                     EIYLDAIPAKKGAAGFARVNYAPPALQAVTRDFAFLLKAEVPAGDLLRAIRGADKANI
                     VAARVFDDFRGAGVAEGEKSLAVEVTLQPGEKSYAEADLKAIAEKVTAAAAKLGAVLR
                     G"
     gene            20956..21840
                     /locus_tag="VFF98_01325"
     CDS             20956..21840
                     /locus_tag="VFF98_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HZU62296.1"
                     /translation="MGDELIAIASGALAARINPLGAELSSLTDAAGAEYMTDADPAYW
                     AGRAPLLFPIVGALAGGHLRHGGKEYALARHGFARRSRFEVVAQTASAVRLLLRDDAA
                     TRVAYPFAFALEMGFALEGLTLRMEARVRNPGGEALPFSFGYHPAFAWPLPGGGAKEA
                     HLIRFAEAEPAPIRRVGAADGLLLPACQPSPVAGRDLALDEGLFAEDAVIWDQLASRA
                     LAYGSETGPWLDIAFPDSPQLGLWQKPGGRFLCIEPWAGHADPEGFAGDLAEKPGIIL
                     LAPGAERAFRMDVSLRPL"
     gene            21852..23408
                     /locus_tag="VFF98_01330"
     CDS             21852..23408
                     /locus_tag="VFF98_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZU62297.1"
                     /translation="MSNASHPSASRRTFAIISHPDAGKTTLTEKLLLNGGAIHLAGEV
                     KARGAARRARSDWMKIEQQRGISVTSSVMTFAREFEGLGHITFNLLDTPGHEDFSEDT
                     YRTLTAVDSAIMVIDAAKGIEPQTRKLFEVCRMRSVPIITFVNKVDREGRSVFEILDE
                     VADALALDVSPQSWPIGMGGTFEGILDLASNRVSRPEGDSREFLGKVETGVALPDEVA
                     EEIELGQAGYPAFDLEAYRHGDLTPVFFGSALKNFGVAELIDAIARIAPPPRPQPSEQ
                     GTIEPTDKEVTGFIFKVQANMDPMHRDRIAFMRLVSGTFKRGMKLTPSALGKPIAIHS
                     PILFFAQDREIADTAEPGDIIGIPNHGTLRVGDTLSEANKVRFTGLPNFAPEILRRVA
                     LKDPTKTKQLRKALDDLSEEGVIQVFYPEIGAQWIVGVVGQLQLDVLISRLEAEYKVQ
                     AMLEPAPFDTARWLKGADNALRDFAEFNKSNLARDRDGAFVFLARSAWDVSYQQERNP
                     ELTFSATKER"
     gene            23631..23969
                     /locus_tag="VFF98_01335"
     CDS             23631..23969
                     /locus_tag="VFF98_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZU62298.1"
                     /translation="MKFIIAIIKPFKLDEVREALGALGVAGMTVSEVKGFGRQKGQTE
                     IYRGAEYSTNMLPKVKIEIAASDELAPRVVETIQQTASTEAIGDGKIFVLDLASATRI
                     RTGETGDTAL"
     gene            23999..>24848
                     /locus_tag="VFF98_01340"
     CDS             23999..>24848
                     /locus_tag="VFF98_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62299.1"
                     /translation="MIRKLICGAGALGTSLFAATTAFAQTAAGAASSAASAVATATAS
                     ATATASAVATPALVDIAKLAADKQSAAEGLLVNKGDTTWMLISSALVLLMSVPALALF
                     YGGLVRTKNMLSLLMQVFMIVSVAALIWVTYGYSMAFTGGSTPFVGGLSKALLMGVSN
                     TTYAATFSNNVYIPEYAYLIFQMTFACITPALIVGSFAERIKFTPLIIFVVLWLTLVY
                     FPMAHMVWYWAGPDFYYTTASTATPDSGYLFTKGALDFAGGTVVHINAGIAGLVGCLY
                     IGKRLGY"
BASE COUNT         3866 a         7958 c         8801 g         4223 t
ORIGIN      
        1 aaggcctatc acggggtgtg agggggggcc ctcacaccgc catattgtcg atcagccggg
       61 ccttgccgat gcgtgctgcc accagcaggc gggcgggcac cgtgccgact gtggccagcg
      121 gcgccaggct gtcggcctcg cgcagttcgg cataatccac cgcgctgaag ccgccggcca
      181 gcaggtctgc ctccagcgcg gccagggctt ccggcacggg cgtgccggcc tggagcgcgg
      241 cgatggcctt gtgcatggcg gcgggcaggc aggcggcctg ctggcgctct gtggccgaga
      301 gataggcatt gcgcgaggag cgggcgaggc catctgcctc gcgcacggtg gcaacgccga
      361 tgatgttttc caccgcgggc cgggccagat caagatcgcg cgccatgcgg cggatcaccg
      421 ccagctgctg ccagtccttc tcgccgaaca gggcgatatc gggcgccacc tggttgaaca
      481 gcttgagcac caccgttgcc accccgtcga aatggccggg ccgggtgccg ccgcacagcc
      541 cctcgctaac gcccgccacc gacacgttgg tggcatagcc ggccggatac atggccgaaa
      601 ccgtgggcgc ccacagcacc gccacgcctt ctgcctccag cagggcggca tcgcgggcca
      661 gctggcgcgg ataggcctcg aggtcctcgc cggcggcgaa ctggcgcggg ttgacgaaga
      721 tcgaggcgac gacctgcgcg ccgcgcgccc tggccgcgcg caccagcgtc agatggcctt
      781 catgcagcgc gcccatggtg ggcaccagcg ccacgggccc ctcggccctc agcgcttcga
      841 gcgcacgccg cagtggatga agctcctcga tgatttgcac gccagcgccc cttacggtag
      901 tttacagttt gtgagtgggg gctctcatgc ctcgccgcgc ctgatatcgc aagccgccct
      961 gtctgggcct caaagggcct gaacctgcga agagagtgtg agccgtgagt gacgcaagcc
     1021 cccccagccc aggcccccat cgcattgttt tcgccaatga aaaaggcgga accgggaaat
     1081 ccaccacggc cgtgcacgtg gccattgcac tggcctatct gggcgggcgg gtggccgcga
     1141 tagacctcga tccgcgccag cgcacgctgc accgctattt cgagaaccgc gccgaaaccc
     1201 tgcgccgccg ccagatttcg ctgccgatga tcagttttgg cgtgttcaat ggctccacca
     1261 ccgaggagct ggatgggctg agcgccgcga tgggcgccgg ccatgattac ctgatctatg
     1321 acacgcccgg ccgcgacgat gtctttgccc gccatgtggc cacccaggcc gatacgctgg
     1381 tgacgccgct gaacgacagt ttcgtcgatt tcgacctcat cggccaggtg gattccgaaa
     1441 gcttccgcgt gcggcgcctg tcgttctatg ccgagctgat ctgggagacg cgcaagaagc
     1501 gcgcgctgag cacccttgcc gataaccgcc gcgagatgga ctgggtggtg gtgcgcaacc
     1561 gcacccagca tatcgaggcg cgcaacatgc gccgcatcga tggcgcgctg gccgaactgg
     1621 cgcggcgggt gggcttccgc attgcccagg gcctgtcgga acgcgtgatc taccgcgagc
     1681 tgttcccctc gggcctcacc ctgctcgaca agggctatct gggcgatctg ggcaccagcc
     1741 atctggtggc ccggcaggaa ttgcgcgcgc tgctctcagg cctcagcctg ccgctgccgc
     1801 ccttcgcgcc ggcgccggcc gccatggcgc cgccgatggg gcaggcgccc atggaacagc
     1861 cggcgatgga acaggcgccc atggaacagg cagggtcaga gcccgcgccc gtgcagccct
     1921 actactgagc cggcggcgca ggcaggccca tgagcaagct gatctggctg gcagcgatcg
     1981 gctgcctggt gttcaaatgg cagaccgggc gctggccgtg gcaggcgcgg ggcgccttgc
     2041 cggggcgcgg ccggggcgca gagcaggcgc tggcccatgc ccgcgcgctg ctgggccttg
     2101 gcgccggggc cagccatggc gagatcatcg aggcgcacaa gcgcctgctg atgatcgttc
     2161 accccgatcg cggcggcacc agcgagcagg tgcatgaggc caccgccgcg cgcgatctgc
     2221 tgctggccga tctggcgcgg cgcagcgctg ccggccagtg atctggtgac ctatcgcctg
     2281 cacccttcca tcctgcggga atatgatatc cgcgggattt gtggcgaaac gctgtggccg
     2341 gccgatgcct ttgtgctggg ctgggccttt gcccgggtgg tggccgaagc gggcgggcgg
     2401 cgcgtggcgg tggggcgcga tgggcggctc agttcgcccg tgctggaagc ggcgctggcg
     2461 gcgggcttgc gggcaggggg cgctgctgtg ctgccggtgg ggcaggggcc cacgccgctg
     2521 ctctatttcg ccgaacggtc aaccgaacag gtggagggcg gcattcaggt aactggcagc
     2581 cacaatcccc ccgatcacaa tggcttcaag atagtattgc agggtgcccc cttcttcggg
     2641 gcggatctcc tccggctcgg gcagatcgcc gaagggttcg atccagctgt cctccccgag
     2701 gggttcggcg cggtgtggca cgggctggct ggcggcgcgg gcgttgatcc gatcctcggg
     2761 gatgccgacg gcgcgggcga aggcggcgag attttccggc gttatatcga tgccctcacc
     2821 gccgcgctgg aggaagctcc ggccggcccg ctggcggccc tgcgcgtggg ctgggatgcc
     2881 ggcaatggcg ctgccggccc ggcgatagag gcgctctgcg cccgtttgcc aggggaacat
     2941 catctgcttt acacccgtgt agacggccat tttcctcacc atcatccaga tccgactgtc
     3001 gaagaaaacc tcgcagattt acgtagcctc gtcctgtcgg aacgcctcga tttcggagtg
     3061 gctttcgatg gcgacggcga ccggctgggg gtgatcgacg ggcacgggcg ggtggtgtgg
     3121 ggcgatcagc tgctgctgct gtttgccgcg gatctgctgg aaagccaccc gggcgcgcgg
     3181 attctggccg atgtgaagac ctcgcaaggc gtgttcgatg gcattgccgc gctgggcggc
     3241 cagccgctga tgtgtgagac agggcacagc tgggtcaaat ccagaatgaa gcaggccggt
     3301 gccctgctgg ccggcgagat gaccggccac ctctttttcg ccgatcgcta tttcgggttt
     3361 gacgatgcgc tgtatgccgc cgtgcggctg atggcggcgg tggcgcggca ggagcgatcg
     3421 gtggccgata tgctggaggc gatgccggtg tgggtgaaca cgccggagct gcgctttgcc
     3481 gtgccggccg cgcgcaaggc ggcggtggtg gccgaagtgg cggcgcggct gcgggccgaa
     3541 ggggccgatg tcgtcaccat cgacgggctg cgggtgcggc gggccgatgg ctggtggctg
     3601 ctgcgcgcct cgaacacgca ggagatgctg gtggcccgcg ccgaaagcgc cgatgcggcc
     3661 ggccttgccc gcctgctggc cgagctggac gggcaattgg ccgcctctgg cgtggcgcgc
     3721 gccggggggc attgatccgt cggcccgggc ccccacattg ggccgatcat ggccgccacc
     3781 gatccccttc cgccagagct ggccggctgg tttgccgggc ggggctggcg cgtgcgcggg
     3841 caccagctgg cgatgctggc cgaggccgat gccggccgcc acgcgttgct gatggccgat
     3901 accggcgccg gcaagacgct ggccggcttt ctgcccacgc tggccgcttt ctgccccagc
     3961 cggcaggggg cggcgccggt gccggaaggg ctgcacacgc tgtatgtctc gccgctgaag
     4021 gcgctggcgc aggatgtgca gcgcgggctg gcccggccgg tggcggatct gggcctgccg
     4081 ctgcgcatcg agacccgcag cggcgacact ccctctgacc gcaaggcccg ccagcgcgcc
     4141 cgcccgcccc atgtgctgct gaccacgccc gaatcgctct cgctgctgct gacctgtccg
     4201 gaaagcgcgg agatgttcgc cagcctgaag cgcgtggtgg tggacgagct gcacgccttt
     4261 gccgccggca agcggggcga tctcctcgcg ctggcgctgg cccggctgca ggtgctggcg
     4321 ccgcagatgc ggcgggcggg cctgtcggcc acgctggccg atgtggcagc ggcccgggcc
     4381 tggctggcgc cgggggacga ggctggcgat gtggcgctgg tgacaggcga ggcaggcgcg
     4441 gcgccggtgg tggagatact ggagccccgg ggccagcgcg tgccttgggc cggccatgcc
     4501 gccgcctggg ccgtgccgca gatctatgag gcgattggcc ggcaccgcac cacgctggtg
     4561 ttcaccaaca cgcggtttct ggccgaatat atcttccagc ggctgtggga tgcgaatgcg
     4621 gatcacctgc cgatcggcct gcaccacggc tcgctcagcc gcgaggcgcg ggcgcgggtg
     4681 gaagcggcca tggccgaggg gcggctgcgg gcgctggtgt gcacgtccag cctcgatctc
     4741 ggggtggatt ggggcgatgt tgatctggtg gtgcagatgg gggcgcccaa gggctcctcg
     4801 cggctgctgc agcgcattgg ccgcgccaac caccggctgg atcagcccag caaggcgctg
     4861 ctggtgccgg gcaatcgctt tgaatatctc gaagcgctgg ccgcgcgcga tgcggtggcc
     4921 gaaggccagc gcgatggcga gggctttcgc cccggcgcgc tggatgtgct ggcccagcat
     4981 gtgatgggcc ttgcctgcgc cggcccgttc gacgaggcgg cgctgctggc cgaggtgcgc
     5041 tctgccgcgc cctatcactg ggtggaggag ggggtgtggc aaagggtcct gcatttcgtg
     5101 gccaccggcg gctttgccct gaaggcctat gaccggttcc gccggctggt gcgcagcgca
     5161 catggcgcct ggcgcctggc ccatcccgag atggccgcgc gccatcggct gaatgcgggg
     5221 atcatcgtcg atgccgagat ggtggaggtg cgctttggca atggccggtc gctgggccgg
     5281 gtggaggagg gctttgccgc ctcgctggcg ccgggcgaca cgttccgctt tgccgggctg
     5341 gaccttgaac tgatgcgcct gggccatgcc gagatgcagg tgcgggcggc caagcgcgcc
     5401 ggtgccatcc ccagctatat gggcgcgcgc attcccatca gcagccatct ggccgcgcgc
     5461 gtgcgggcga tgctggcgcg cccggcgctg tggccggcgc tgcccgacga ggtgcgcgaa
     5521 tggctggaga tgcaggccca tgtggcgcgc ctgccgggcg aggaggggct gctggtggaa
     5581 agcttccccc atgccgggcg ccatcacgcg gtgttctaca cgttcgaggg ctggccggcg
     5641 aaccagtcct tgggaatgct catcacccgg cggatggaag cgcaggggct ggcgccgctg
     5701 ggctttgtgg ccagcgatta tgcgctggcg gtgtggggcc tgcggccgat agaggcgccc
     5761 ggcgccctgc tttcggccga catactggcc gaggaatttc tgccctgggt gcgcgaatcg
     5821 catctgctgc gccgggcctt ccgcgaggtg gcggtgattt ccggcctggt ggagcgccag
     5881 cagcccggcc agcgcaagag cgggcggcag gtgcgctttt ccaccgacct catctacgac
     5941 gtgctgcaga aatacgagcc cgaccacctg ctgatcgagg cggcctgggc cgatgcccgc
     6001 cagcgcctga ccgatgtggc ccgcgtggcc gaggtgctgg agcgggccgg gcgggcaatc
     6061 gaacatgtgc cgctggcccg cgtcagcccg ctggcggtgc cggtgctgac gatgatcggc
     6121 cgcgaggggc tgccggccgg cagcggcgag gaggaactgc tgttcgaggc cgaggcgctg
     6181 gcggcgctgg ccatgggcga catggaagcg tgcgccgccg cccctcagtg atggcaggcg
     6241 agcgcttcca ccacctcttc cagccgcgcc ggatcgccca gcgccagcac atggccatcg
     6301 ctgcccatat gcagcgggtc gccgttccag tcggccatgg tgccgccggc gccttccacc
     6361 accggcacca gcgcggccca gtcgtgcagc ttgaggccgg cctcgcagat gatgtccacc
     6421 tcgccgctgg ccagcatggc atagttgtag cagtcgccgc ccatcaccat gcggcggtgg
     6481 tcggtcttgg cggccagggc catgaagtgg gcgccctcgt gctcgtcgaa ataatggggg
     6541 ccggtggtgg cgatattggc atcggccagc tggcggcagg cgcgggtgtg ggcgggcttg
     6601 ccgttcagca gggtctgata gcccgaggcg ccgacccagc gctcgttgag gatgggctga
     6661 tcgatgatgc ccagcaccgg ccagccatcc accaccagcg cgatcagcgt gccgaagatg
     6721 ggccggccag cgaggaagcc tgccgtgccg tcgatcggat cgagcaccca ctggcgggtg
     6781 gcgccggcgc cttcatggcc gaattcctcg ccgatgatgc catcgcgcgg ggcctcggcc
     6841 ttgagcaggc ggcgcatggc ttcctcggcg gcgcggtcgg ccagcgttac cgggctggca
     6901 tcgtccttgc gttcggagga gaggccggcg cggaaatgcg ggcggatggc agcgcccgcc
     6961 gcatcggcaa ggcgcatggc aagggcgata tcggcatcga gtttcatggc ccctgccttg
     7021 cccgaaaagc gcgcgggcga aaaggggggc tggcacccgc aggcgccagc gccggccttt
     7081 agtcgaacgg gcctttagcc gaacaggccc ttagtcgaac aggctggaga ccgagctttc
     7141 gtcggcgatg cggcggatgg cctcgccgat cagcggggcg atggtgagca cgcggatctt
     7201 gtcggactgc tgggtggcct cggtggcgag gatggtatcg gtgatgacca gttccttcag
     7261 cgccgaattg ctgacccggc tgaccgccgc gcccgacagc acgccatggc tgatataggc
     7321 cgtcacggaa gcggcgcccg catcgatcag cgcctgggcg gcgttgcaca gcgtgccgcc
     7381 cgaatcgatg atgtcgtcga tcatgatgca ggtgcggccc ttcacgtcgc cgatgatgtt
     7441 catcacctcg gagacgcccg gcttgtcgcg gcgcttgtcg acaatggcga gcggcgcgtt
     7501 gttgagccgc ttggccaggg cgcgggcgcg caccacgccg cccacgtccg ggctgatcac
     7561 catcaggtcc ttgtcgccat agcgggcctg gatgtcggcc gccatggtgg gggcggcgaa
     7621 cagattgtcg gtggggatat cgaagaagcc ctggatctgg ccggcgtgca gatccaccgc
     7681 cagcacgcga tcagcgcccg ccgtggtgat cagattggcc accagcttgg ccgagatcgg
     7741 cgtgcggggc ccaggcttgc gatcctgccg ggcatagccg aaatagggca ccaccgcggt
     7801 gatccgcttg gccgaggcgc ggcgcagcgc atcgatgcag atcagcagtt ccatcagatt
     7861 gtcgttggtg gggtagctgg tcgattgcac gacaaagaca tcctcgccgc ggacattctc
     7921 gtggatctcc acgaaaactt cctcatcggc gaagcggcgc acggcggcat ccgtgagcgg
     7981 cagttccaga tagcccgcga tcgcgcgtgc gagcggcagg ttgctgttgc cggccatgat
     8041 cttcattgcg tgtgctctct ttgcttgctg ccccgaacgt ccggtcctcg ggcgaatccc
     8101 ctgggggcga ctcgcatgcc ggcgcgcccg cccttagccg acagtcacgc ttttgcaata
     8161 tgggcgtggg ggcggggcgg cgatccgccc cattttcgca caggaaaacg cgcccccgat
     8221 ttctggcgcc cgattgctga tgcccgatta ctggcggcga tcgcccttca cccagcgcac
     8281 ggtgcccgag gaagcgcgca tcaccacgct gtcggtcgtc atgcggccgt tcttcagctt
     8341 cttcacgccc ttcagcagcg aaccgtcggt cacgccggtg gcggcaaaga tgcaatcgcc
     8401 cttggccagt tcggtgaggt cgtagatctt gttgagatcc tcgatgcccc acttcttggc
     8461 gcgcaggcgc tcgtcctcgt tgcggaacac caagcggccc ttgaactggc cgcccacgca
     8521 gcgcagcgcg gcagctgcca gcacgccttc cggcgcgccg ccctggccca tgtagaggtc
     8581 gatattggtt tcctcattgg tggtggcgat cacgccggcc acatcgccat cgccgatgag
     8641 gtagacgccg cagccgatcg cgcgcagctc ggcgatgaga tcggcatggc gcgggcgatc
     8701 gagcacgcac accacgatat cctcgggctg cacgcccttg gccgcggcca ccgcctgcac
     8761 gttttcggtc gggctcttgt cgaggtcgat gatgttttcg ggatagcccg ggcccaccgc
     8821 cagcttgtcc atataaacgt cgggcgcgtt gagcaggccg ccttcctcgg cgatcgccag
     8881 caccgccagc gagttggggc cggccttggc gcagatggtg gtgccttcca gcgggtcgag
     8941 ggcgatatcg atcttggggc ccttgccgat ggcattgccc accttctcgc cgatataaag
     9001 catcggcgcc tcgtcgcgct cgccctcgcc gatcaccacg gtgccgtcca tgtaaaggtc
     9061 gttcagcttg gcgcgcatgg cttccaccgc ggcggcatcg gcggccttct cgtcgccccg
     9121 gccgatcagc ttggcagaag cgatggcagc ggcttctgtc acgcgcacca tttcgagcac
     9181 gagaacacgg tcaagaacct tgctggcggg ggtagcggtc atatcagtcg caatcctcta
     9241 ttcgcggtgc cacgcatagt tatgcatagc tatgcatgtg gcaaagccgc ttaaacaggc
     9301 cggcgccgct tgtcgagagc ggccagttgg ccgggtcagc cagccgaata acccggcttt
     9361 gttacaatca tccaaataag cgccccgata cgccgcagcg atcaggcgcc caatatctgc
     9421 atcaccagcg gggcgccggc aaggctggcg gaaccttcca gcagcgccag cgcccgggtg
     9481 acggcgcttt cgggcccttc atgcgtgacc atcgccacca gcacctggcc gccggcctcg
     9541 gcgcggccgc gctggctgaa gctggcgatg gaaacgccgg catcgcgcat ggccgcggtg
     9601 atctcggcca gcacgccggg ccggtcttcc acgaggaagc ggatataggc acagccggcg
     9661 cgcgcgccgc tgttggccgg cgccagcggt tccagctggg tgacggggac ggagaagctc
     9721 atgtcggctt caaagccggt gtccatgccg cgggcgatgt cgatgatgtc ggccaccacg
     9781 gccgaggcgg tggggccatc gccggcgccg gcgccctgga acagcagctt gcccgagaaa
     9841 ttgccttcca ccaccactgc attggtggcg ccatcgacat gggcgagcag gttgtcgaac
     9901 ggcacgagat ggggctgcac gcgctggaac aggccgccgg tgccgctttc agccccgctc
     9961 tctgccccgc tttcggccat gccgatcagg cggatgacat agcccagcgc attggcctcg
    10021 gcgatatcgg cggccaggat gcggctgatg ccgctggtgt ctaccgaggc gaaatcgatc
    10081 cgcgcgccaa aggcaatggc cgagaggatg gagagcttgt gcgccgcatc cgtgccctcg
    10141 atatcgaagg tggggtcggc ctcggcatag cccttggcct gggcttcggc gagcacctcg
    10201 gcgaaatcgc ggccggtatc ctccatggtg gagaggatat agttgcaggt gccgttgagg
    10261 atgccataga tccgctcgac gcggttggcc gcaacgccct cgcgcaggcc cttgatgacc
    10321 gggatgccgc cggccaccgc cgcctcgaag cgcagggcaa cgccggcctg ttcggcagcg
    10381 ccggccagtt ccatgccatg gtgggcgatc atcgccttgt tggcggtgac cagcgccttg
    10441 ccgtggcgaa tcgcgttgcg ggccagggtc agcgccgggc catccgagcc gccgaccagt
    10501 tccaccacca catccacgtc atcgcgcgcg gccatggcgg tcatgtcgtc ttcccaggca
    10561 tagggcgaca gatcaacgcc gcgatccttg ccgcgttcgc gcgcggagac ggccaccacc
    10621 tgcaccgggc ggccggcgcg ccgggcaatc agcgcggcat tggcctcgat caggcgaatg
    10681 acacccgcgc ccaccgtgcc aaggccggcc agggcaatgc gcagcggcgg ccgcgcggcg
    10741 ggagaggagc tgtctgccat gatgatccct ttgctgcccg cgccatgcgg gccaagctgc
    10801 aaaaagcgct ccgatagggc cctatggcgc ccttcgcaat ggggaagggc ggcctgcggg
    10861 gctcagtcgc gcaggcggcc gcaggggccc agttcggcca gcaccagccg atagtcgcgc
    10921 tcggcctgga ggcgggcatc gggcgtggcc gagatgttga tgccatcggg caggcgcgcc
    10981 ggcaggaagc aggccagccg ataccaggcc agcgtatcat gcgcaggggg cggcgcatcg
    11041 gggttcacca cctcggagaa ggagaccgac cagtgcaccg gctggtccgg ctggtgcaac
    11101 acgctgatcg aggccggctc gcccttggcg gtggccagga acagctgggt ctcgccttcg
    11161 ccggccagcg tgccgggctc atacagcgcc tgggtgatgg cggtgacagg cggcgcggca
    11221 tccggcgcga acagatcgcc gatcgccttg tgcacgcgat cgaccagcgc gggatcggcc
    11281 agccactgcg catcggcggc caccagctgc aggtcctcgg cgccgcgcgc cagcgtgtgg
    11341 gcgaacagca gcacctgctg cttcttcagc gagggcacct tgcctttggc atccagcggc
    11401 acatcggcca gataatgcac cgattcggcc agcggcacat cgccgcgcaa aatcaccgcc
    11461 ggccgcgctt ccacatagat gcgcgcccag cccggtttca cgccgggcgc gcgggccggc
    11521 tccaccgtgg cctggccgcg cacttcggcc tgcatcacca gccgggccga atcagcaagg
    11581 tcgacaatgt cggcataggc atggggggca ggcgcggggg gtgggggggc tgccctggca
    11641 aaaggaacag ctccgccgag aatcgccgcg gcaaacgctg cgctgcacca tacaggccct
    11701 gccattttca ttgctcaaat ccccggatag tccgcaaaat attttaagcc atggcattaa
    11761 catagctgaa tcgacggtta accccgcact tatgccgata agccgttgca cgggtgccac
    11821 atcgacgtta aacgtcctgc aaaactgggc caaggctgat tttggcccac aataagcatg
    11881 ggggcggcgc tagcgccgcc cttggcattc cggaatttcc ggcagggtgg aatatgagtt
    11941 ttcgcttcag catccggttt gccggaggtt ggggcatggc cgggattttc ccgggggttg
    12001 tgcatggagt gatgtggctg aatggcttac gctgatcaac agatgagcgg ttctagggta
    12061 accgcgctta tcatcgttgc cttgattcac gtcgcagtcg gttacgcgct gatcactggc
    12121 cttgcctatt cggccgccaa gcagatcatc aagaaagtga ccacggtcga catcaaggag
    12181 gacaaaccga aaccgccgcc gccgccgccg ccgccgaagc aggacgcgcc gccgccgccc
    12241 attgtggcgc cgccgcctcc tgtgaacgtg agcgtggcgc cgccgccggt gcaaaccgtg
    12301 tcggttgcgc cgccaccggc tccgccggtg ctggcgccgc ctgcgccggt ggcaccgccg
    12361 ccgcccaagg tggccgcgca acgcgcggtg ccgcgcggca atccgggcga ctgggtgacg
    12421 ccggaagact atccctcgcg tccgctgcgt gaaggcgtgc agggtgtgac cagctttcgc
    12481 gtgacgatcg gccccgacgg ccgcgtgacc ggttgcgaaa tcaccggttc gagcggcaat
    12541 gacgagctgg acaaggcgac ctgcacctat tttgcaaggc gcgcccggtt caagccggcg
    12601 gtgggcagcg atggtcagcc aatgaccgac agctatgcca accgcgtcaa gtgggtcatc
    12661 ccgaaggact gacctgcggc cttatcccaa tcccccctac aggggcgcgg ctgatccacc
    12721 cgccccgctg ttcatctttt tcttgagagg accatcgcat gcttactgaa attctcgccg
    12781 ctggcgccaa ctcggccccg cagaacaagt tcggcttcat ggaagccatg cagcagggtg
    12841 gcgccctgtc gtggtcgatc gtcaccatcc tggcgatctt ctcgttcggt tcgttcttca
    12901 tcctgttcac caagtgggcg gaccagtcga agatcctcaa gcagtatggc aatctgaacg
    12961 ccttctggaa ggccccgacc ctgcaggaag gcctggccaa gctcgacaag cgcagcgcgt
    13021 tccgcgacct cgtcgaagcc ggtctggctg ctgaagagca gcactcgaag ctggttgatg
    13081 ccctggaagc ccatgactgg ctgcacggtt cgctcgcccg cgccgagaac agcatcactt
    13141 cctacctgtc gcgtggcctg cccttcctcg ccaccgtcgg ttcgaccgcg ccgttcgtcg
    13201 gcctgttcgg caccgtggtc ggcatctacc gcgccctgat cgccatcggc atcgccggtt
    13261 cggcctcgat cgacaaggtg gccggccccg tgggtgaagc gctgatcatg accgccatcg
    13321 gtctgctcgt cgccgtgccg gccgtgcttg cctacaactt cctgatgagc cgcaacaagc
    13381 gcatcaacga agtgctgtcg gccttctcga acgatgttct ggcgaacatc aactcgaagg
    13441 gtgcgatcaa gcctgccgtt gctgctgcca aggctgctcc ggtcgccgcc aagccggccg
    13501 gcaagtaatc gcctgatgat ggagcgccgg cgccccttgc ggggcggcgc cgggcccagg
    13561 cgggaagggg tgggccgtgc ggcatgccac gatccgctcc tcccctcccc tgacggggat
    13621 catgcaagga taggaattca cccatggcaa tgtcagtagg cggcggcggc gaagacaagc
    13681 cgatgtccga catcaacacc acaccgctgg tggacgtgat gctggtgctg ctgatcatct
    13741 ttctgatcgc ggtgccggtc gcgatccaga cgatccagaa gctcaagatc ccggtgatgg
    13801 tctcgcaaga atcgaaggac aaggtggaga atctgctgct gaccgtggca accaccgatg
    13861 aagctggcca cagtgccggc gacccgggtt acaccggttc gtcgcgcggg ggcgagtgcc
    13921 ggatctattt caacaacatc acccctgtca cctcggatga actgcgcgac aaggcgtaca
    13981 agcgccttga tgccatcgtg aagcgtgaag gtggcgccga agcactgcgg gccgagcccg
    14041 acaaggtgcc ccaggtgcat atccgtgccg acgtgaacgc gccctggcgc tgcgtggccg
    14101 gtgccatcta caatgtccag gtttcgggct atcccaccgt tggcttcatt tcgaccccgg
    14161 tcgatccgaa cggctgacgc gcgcggcaac ttacaggagc aactcctatg gctatgtcag
    14221 gcggcaagga cgatggcgag ccgatgaacg aaatgaacac gacgccgttg atcgacgtca
    14281 tgctcgttct gctcatcatg ttcatcatca ccattccggt ggccacccat gcggtgaaca
    14341 tcgacctgcc ttcccccagc ccgaccccgc ccgatatcaa ggtggagccg gtgaagaaca
    14401 aggtgaacgt cacggcggat ggcaagatcc tgtgggatag caatcccgtg gatcagggcc
    14461 agctgctgac gctgctgcag cagtcgacgc agatgacccc ggaacccgaa ctgcagttcc
    14521 agcccgaggc ccaggccggt tacgacctga cggctcacgt gctgaacatc atcaagggtt
    14581 cgggcgtgac caagttcggc tttgtcggca acgagcagta tgcctcgttc agcaaggctg
    14641 ccaacgcgga caaggcgggc gaataagccc tcaccttgct tgcgatgcga tgaggggcgg
    14701 agaaatccgc ccctttttcg tgtcaggcgt cggcgtaggt ggcgacttcg ggcttgccct
    14761 ccagcgcggc catgagcgcg gccatggcgg ccttggggag cagcggggcg gcggccttgc
    14821 gggcctcttc gctgtcccag aattcgagga agaggaagtg ggcgggggcg tctgcccggc
    14881 gcaggatgcg ggtgccttgc gtgccggggg cggcggcgag ggctgcggcc agttcggcaa
    14941 gggcggcggc cagcgcggcg gcgctgtcgg cgcgggcggc aagcctgtag gtttgaacga
    15001 tggccatggc attttgctcc caaagaatgc gccaaggttt gcagatggct gccggcgcgg
    15061 caagatgggg gcatggccgc cgctttgccc ttgcgcttct ttttctatgg cacgctgatg
    15121 ggcgggcatg gcaatgccgg cgcgcaggtg gtggccgccc atatgcgcct gctgggcgcg
    15181 gcgcgggcgc cgggccggct ggtggggctg gaggagggcg ggggctggta tccggcgctg
    15241 ctgcctgatg ctgggggcgg gcaggtggcc ggccggctgt atgaggcgct gccgggcttt
    15301 ggcgcggcgc agctggccgc gattgaccgc tatgagctgt gttttcccga ggatccgggc
    15361 gtgagcgaat attgccgtgc gctggtgccg gtggcctggg ccggcgggca gggggaggcg
    15421 caggcctatc tctatgcgcg ggccttgccg cccggggccc tgccgatcgc ggggggcgat
    15481 tttgcccgct ggctggccga taccgggcgc agggcctttg gtgcggcggg ctgagggcgc
    15541 acagtgcttg caattgccgg ccattgccct taagggccgc gccttcgaac ggctcggttg
    15601 agtctccgct ttggggattc gctgtgcgcc tggccaacta gggctgccac ggcgcgcatt
    15661 tccgggccct gcaaaggaga cgaaaatgcc caagctcaag accaagagcg gcgtcaagaa
    15721 gcgcttcaag ctcaccgcca ctggcaaggt gaagcacggc gttgccggca agcgccaccg
    15781 cctgatcagc cacaacggca agtatatccg ccagaaccgt ggtaccgacg tgatttccga
    15841 cgctgatgcg aaggtgatca agaagtgggc gccctacggg ctgaactgag gagactagcc
    15901 gatgtcccgt atcaagcgtg gtgttaccac tcgcgccaag cacaagcgga ttttggatca
    15961 ggcgaagggc tatcgcggcc gtcgcaagaa caccattcgc gttgcccgtc aggccgtcga
    16021 aaaggctggc cagtatgcct atcgcgatcg caagatcaag aagcggacct tccgcgccct
    16081 gtggatccag cgcatcaacg cggctgtccg cgctgaaggc ctgacctatt cgcagttcat
    16141 caacggcatc aagctggccg gtatcgagct ggaccgcaag gccctggccg atctggcgat
    16201 gaacgagggc gcgatcttct cggccgtgct cgctcaggcc aaggctgccc tgccggcggc
    16261 ctgaagcctg cacatggcgc tgccggtgtg atgccggcgg ccatgggcta aacaaaaagg
    16321 cgcgaatcgc ataggcggtt ggcgcctttt ttgcgtccgc aatggtggcg gattatggct
    16381 aagggattgt caatcgggga aatttgtagc aggtgaagga aacaagacag ggggcgcaat
    16441 gcagaaaaga tgcacagtgg aggtcactta ttacccgcct ccgcttgcgc ttagtcccta
    16501 ttttccgaca tttgccctgg ctgaagtcga aatcgacggg ccgggcctga tcaccgatta
    16561 cttgcacccc gaatggggaa cgatccgatt ccatgcagga acgcctgcgc atacggtgga
    16621 ttacctcggg cgtaaccagc cggatctgtt cttttccgta acagggccca gcacccgggc
    16681 ggtgcgcttc acggtgggca gttgccgcat ctggggcatt ggcatgccgc cgctgggctg
    16741 ggcgcggttt gtgggcctgc cggcggcagg cacggccgac agggtgctga acgggcagca
    16801 cgagccggcg ctggcccgct ttctgccgct ggcgcagggg ctgtatggcg aagagcgcgc
    16861 ggtggagcgc gagctggcct gcctgaccga tttcttcctg gcgctggatg ccggcctgcc
    16921 gcccgtgccc gatgccgagc gtattgtggc ggtgcatgca gcgctcgttg atcatgaagt
    16981 gtcgagcgtg gcgcagttgg tggagcgggt gggcttttcg cacagcacgc tggagcgcct
    17041 gtgtcaccgc gctttcggct ttgcgcccaa gcagttgctg cgccgccagc ggtttatccg
    17101 cagcctggcg caatatatgc tcgatccctc gctgaagtgg attggcgcgc tggatgggca
    17161 ttaccacgat caggcgcaat tcgtgcgcga gttccgcgct ttcatgggga tgagcccgcg
    17221 gcaatatgcg gcgctcgatc tgccggtgct gcgggcggcc acgcaggccc gccagcgcct
    17281 ggccggcgcg ccgatgcagg cgttgaccgg ccctgtctcg cccgaaccca ttgcccttgg
    17341 ccgcgccggc tcgtaaggac ggcgcgacag gcgcccgggg cgccggcctg cccctgcacg
    17401 ggcacgcaac aggacggatg tgacgacgtg gactacgagg gcatgggcgc cgaggcgctg
    17461 gagaaaatcg cggcggcggc cgatctggac gggctggaga cgctgcggat cgcctatctg
    17521 ggcaagcagg gttcggtttc ggccctgctg aagacgctgg gcgcgatgag cccggacgag
    17581 cggcaggcgg tgggcccgcg catccacgct ctgcgtgagg gcgtgacggc ggcgctggcc
    17641 gaacgcaagg cggcgctgga aggcgcggcg ctggacgcgc gcctggcggc cgagacggtg
    17701 gacctgtcgc tgccggcccc tgtggcgccg cgcggcagcg tgcacccggt ttcgcaggtg
    17761 atggacgagc tggccgagat cttcgcggat ctgggctttg cggtcgccag cgggccggag
    17821 atcgaggacg actggcacaa tttcaccgcg ctcaacatgc cggaaagcca tccggcccgc
    17881 gcgatgcacg acactttcta cctgcccgac ctgacggcag agggccgcga gatggtgctg
    17941 cgcacgcaca ccagcccggt gcagatccgc gccatgctgg ccgctggcgg caaccagccc
    18001 ttgcgcatca tcgcgccggg ccgcgtctat cgcagcgata gcgatgccac tcacacgccg
    18061 atgttccatc aggtggaagg gctggtgatc ggcaaggata tccatctggg ccatctgaag
    18121 tggacgctgg agacgctgct gaaagccttc ttcgagcgcg aggatatcgt gctgcgcctg
    18181 cgccccagct atttcccctt caccgagcct tcggtcgagg tggacaccgg ctattcctgg
    18241 caggacgggc gccgcgtggt gggcggcagc ggcgatgcgc cgggccattc gtggatggag
    18301 ctgctgggca gcggcatggt caacgctcat gtgctgcggg cagggggcta tgatccggcg
    18361 gaatggcagg gctttgcctt tggcgtgggc gtcgatcgcc tggccatgct gaaatatggg
    18421 atgaatgatt tgcgggcctt cttcgatggc gatcagcgct ggctgtcgca ctatggtttc
    18481 agcgcgctcg acgtgccgac gctgtcggcc ggcgtaggcg tgccggcgta agggggcggg
    18541 gatatgaaat ttgcgctttc ctggctgaag gcctgcctcg acaccgatgc cggtgtggac
    18601 gagatcgccg cccggctgaa tgccatcggg ctggaagtgg aaggggtgga ggacccggcc
    18661 gagaagctga agggcttccg cgtggccagg atcctgaccg ccgatcgcca ccccaatgcc
    18721 gacaagctgc aggtgctgag cgtggacacc ggcgagggcg cgcccttgca ggtggtgtgc
    18781 ggggcgccca atgcgcgggc cgggctggtg ggcgtgctgg ggctgccggg cgcggtggtg
    18841 ccggccaatg gcatggtgct gaaggtggcc gcggtgcgtt cggtcgaatc gaacggaatg
    18901 atgtgctcgc tgcgcgagct ggagctgggt gagggccatg acggcatcat cgagctgccg
    18961 gaggatgcgc ctgtcggcac ggattttgcc agctatcatg gcgccgatcc ggtgttcgat
    19021 gtggcgatca cgcccaaccg gcccgattgc atgggcgttt atggcattgc ccgcgatctg
    19081 gcggcggccg gccttggcac gctgaagccg attgtggcgc ccggcattgc cggcagcttt
    19141 gcctgcccgg tggagatccg caccgaggat gccgagggct gccccgcctt ttatggccgg
    19201 gtgatccgcg gcgttgccaa tggcgagagc ccggcctggc tgcaggcagc gcttgccagc
    19261 gccgggcagc ggccgatctc ggcgctggtg gacatcacca actatgtgat gctgacctat
    19321 ggccgcccgg cccatgccta tgatctggca aggttgcagg gcgcggtggt ggcgcgccgg
    19381 gcaaaggccg gcgaaagctg cgtggcgctg aacggcaaga cctatgcgct ggacgagacg
    19441 atgacggtga tcgccgatga ggcgggcgtg cacgatatcg ccggcatcat gggcggcgaa
    19501 cattccggct gcacggccga gacgcgcgat gtgctgctcg agatcgccta tttcaacccg
    19561 gagcggattg ccgccaccgg ccgcaagctg aacctcacct cggatgcgcg gggccgcttc
    19621 gagcgggggg tggacccggc cttcctcgag accgggctcg acctgctgac cgggctggtg
    19681 ctggagatct gcggcggcga ggcatcggac gtggtgcggg cgggcagcgc gcccgtggcg
    19741 ccgcggcagg tggcctatga tccgcgcctg gccgaaacgc tgggcggggt ggcgattgcg
    19801 gctgaggagc agcggcgcat tctggccgcg ctgggctttg ccgtggccga ggactggacg
    19861 gtgaccgcgc cgggctggcg gcccgatgtg gatggcgcgc ccgatatcgt ggaagaggtg
    19921 gtgcgcatcc acgggctgga caatatcgcc agcgtgcctt tgccgcgggt ggatggcgtg
    19981 gcgcggccga cggcaacgcc cgcccagaag ctggagcggc gcctgcgccg ggcggctgcc
    20041 gcgcgcgggc tgaacgaggc tgtcacctgg tcgttcctgc cgatggcaga ggccgggcat
    20101 tttgccgcgg gcgacctctg ggtgctgtcc aacccgatca gcgaggacat gaaggcgatg
    20161 cgcccctcgc tgctgccggg cctgctgatg gcggcgcggc gcaatctggc gcgaggcgcg
    20221 gccggccttg gcctgttcga gatcggccgg cgctatctgc gcgggagcga tggccgcagc
    20281 gatgaaaagc tgacgctggc cgtggcgctg gtgggcgaga aggccgcgcg cggctgggat
    20341 gcgcgcgcca atggcggcaa ggcgcaggcc ttcgatgcgt ttgatgccaa gggcgcggcg
    20401 ctggcgctgc tggccgaggc gggcgcgccg gtggacaatc tgcagatcat gggcgaggcg
    20461 gggtcgcagt tccatccggg ccagtctgcc accttgcggc tggggccgaa gaatgtgctg
    20521 gcgcgctttg gcatgctgca cccggccacg ctgaaggcct ttgacatcga ggtgccggtg
    20581 gcggtggccg agatctatct cgatgccatt ccggcgaaga agggcgcggc cggctttgcc
    20641 cgggtgaatt atgccccgcc ggcgctgcag gcggtgacgc gtgactttgc cttcctgctg
    20701 aaggccgagg taccggcggg cgatctgctg cgcgccattc gcggggcgga caaggccaat
    20761 atcgtggcgg cccgcgtgtt cgacgatttc cgcggggcgg gcgtggccga gggcgagaaa
    20821 tcgctggcgg tggaagtgac gctgcagccg ggcgagaaga gctatgccga ggccgacctc
    20881 aaggcgattg ccgagaaggt gacggcagcg gcggccaagc tgggcgcggt gctgcgcgga
    20941 taagggagag ggcgcgtggg ggatgagctg atcgcgattg cctcgggcgc ccttgccgcc
    21001 cggatcaacc cgctgggggc ggagctgtcg tcgctgacgg atgcggccgg cgcggaatat
    21061 atgaccgatg ccgatccggc ctattgggca gggcgggcgc cgctgctgtt ccccatcgtg
    21121 ggggcactgg cgggcggcca tttgcgccat ggcggcaagg aatatgcgct ggcccgccat
    21181 ggctttgccc gccgcagccg gttcgaggtg gtggcgcaga cggcatctgc cgtgcgcctg
    21241 ctgctgcgtg atgatgcggc cacgcgggtg gcctatccct ttgccttcgc gctggagatg
    21301 ggctttgcgc tggaggggct gaccttgcgg atggaagcgc gggtgcgcaa tccgggcggg
    21361 gaagcgttgc ctttcagctt tggctatcac ccggcctttg cctggccgct gccgggcggc
    21421 ggcgccaagg aagcgcatct gatccgcttt gccgaggccg agcccgcgcc gatccgccgg
    21481 gtgggggcgg ccgatggcct gttgctgccc gcctgtcagc cgagccccgt ggcgggccgc
    21541 gatctggcgc tggacgaggg gctgtttgcc gaagacgccg tgatctggga ccagctggcc
    21601 agccgggcgc tggcttatgg gtcggaaaca ggcccctggc tcgacatcgc ctttcccgat
    21661 agcccgcagc tgggcctgtg gcagaagccg ggcgggcgct tcctgtgcat cgagccatgg
    21721 gcgggccatg ccgatcctga aggctttgcc ggggatctgg ccgagaagcc cggcatcatc
    21781 ctgctggcgc cgggggcaga gcgcgccttc cgcatggacg tgagcctgcg tccgctgtaa
    21841 gcggcgcggc catgtcgaac gcctctcacc cttccgccag ccgccgcacc tttgccatca
    21901 tctcgcatcc ggacgcgggc aagaccacgc tgaccgaaaa gctgctgctg aacggcggcg
    21961 ccatccatct ggccggcgag gtgaaggcgc gcggcgcggc aaggcgcgcc cggtcggact
    22021 ggatgaaaat cgagcagcag cgcggcattt cggtcacctc ctcggtgatg accttcgcgc
    22081 gcgagttcga ggggctgggg catatcacct tcaacctgct cgacacgccg ggccacgagg
    22141 atttttccga ggacacctac cgcacgctga cggcggttga ttcggcgatc atggtgatcg
    22201 atgccgccaa gggcatcgag ccgcagacga gaaagctgtt cgaggtgtgc cgcatgcgca
    22261 gcgtgccgat catcaccttc gtcaacaagg tggaccgtga ggggcgctcg gtgttcgaga
    22321 tcctcgatga ggtggccgat gcgctggcgc tggatgtcag cccgcagagc tggccgatcg
    22381 gcatgggcgg cactttcgag ggcattctcg atcttgccag caaccgcgtc agccggcccg
    22441 agggcgacag ccgcgaattt ctcggcaagg tggaaaccgg cgtggccctg cccgatgagg
    22501 tggccgaaga gatcgagctg ggccaggccg gctatcccgc cttcgatctg gaggcctatc
    22561 gtcatggcga cctcacgccg gtgttctttg gctcggcgct caagaatttc ggcgtggccg
    22621 agctgatcga cgcgatcgcc cgcatcgcgc cgccgccccg cccccagccc agcgagcagg
    22681 gcaccatcga gccgaccgac aaggaagtga ccggcttcat tttcaaggtg caggccaata
    22741 tggacccgat gcaccgcgac cgcatcgctt tcatgcggct ggtgtcgggc actttcaagc
    22801 gcggcatgaa gctgacgccg agcgcgctgg gcaagcccat cgccatccac tcgcccatcc
    22861 tcttcttcgc gcaggaccgc gagattgccg atacggccga gcccggcgat atcatcggca
    22921 tccccaacca cggcaccttg cgcgtgggcg acacgctgtc ggaggccaat aaggtgcggt
    22981 ttaccggcct gcccaatttc gcgccggaaa tcctgcgccg cgtggccctg aaagacccga
    23041 ccaagaccaa gcagctgagg aaggcgctgg atgacctctc cgaagagggg gtgatccagg
    23101 tgttctatcc cgagatcggc gcgcaatgga tcgtgggcgt ggtgggccag ctgcagctgg
    23161 atgtgctgat cagccggctg gaggcggaat acaaggtgca ggcgatgctg gagccggcgc
    23221 ctttcgacac cgcgcgctgg ctgaagggcg cggacaatgc gctgcgcgat ttcgccgagt
    23281 tcaacaagag caacctggcg cgcgaccgcg atggcgcttt cgtgttcctc gcgcgttcgg
    23341 cctgggatgt gtcctatcag caggagcgca accccgagct gaccttcagc gccaccaagg
    23401 agcgctgagc cgggccgccc ctgcctgccg gcgcggcagg cagggctgat agctgtgcct
    23461 aaattttggg catatgcgca agattcgggc gcctcttttt ccgccctttg tgcagatgcg
    23521 aaggggtggg cggcataaat cccccaattc tcaccaattc gtaaattggc acgccccttg
    23581 cgatgtctcc ttcgccggca aaagggccgg tgggctagaa gggggcaggg atgaagttca
    23641 tcatcgccat catcaagccg ttcaagctgg acgaggtgcg tgaagctctc ggcgcactgg
    23701 gcgtcgcagg catgacggtt tccgaggtca agggctttgg gcggcagaag gggcaaaccg
    23761 agatctatcg tggggccgaa tactccacca acatgctgcc caaggtgaag atcgagatcg
    23821 ccgccagcga cgagctggcg ccgcgtgttg tcgagacgat ccagcagacc gccagcaccg
    23881 aggccatcgg tgacggcaag atcttcgtgc tggaccttgc ttctgcgacc cgcattcgca
    23941 ctggcgaaac cggggatacc gcgctgtgag cgccgccagc ctttggaggg agaccacgat
    24001 gatccgcaag ttgatctgcg gcgctggcgc actgggcact tcgctctttg ccgccaccac
    24061 cgcttttgcg caaacggcag cgggggctgc ctcgtcggcg gccagcgccg ttgccaccgc
    24121 caccgcttct gccaccgcca ccgcatcggc cgttgccacg ccggccctgg tcgacatcgc
    24181 caagctcgct gccgacaagc agagcgcggc tgaaggcctg ctggtgaaca agggtgacac
    24241 cacctggatg ctcatctcct cggcgctggt gctgctgatg agcgtgccgg ccctggcgct
    24301 gttctacggc ggcctggtgc gcaccaagaa catgctgagc ctgctgatgc aggtgttcat
    24361 gatcgtttcg gtggccgcgc tgatctgggt gacctacggc tattcgatgg ccttcaccgg
    24421 cggttcgacg ccgtttgtgg gcggcctgtc gaaggcgctg ctgatggggg tgagcaacac
    24481 cacctatgcc gcgaccttct cgaacaacgt ctacattccc gaatatgcct atctgatctt
    24541 ccagatgacc ttcgcctgca tcacgccggc gctgatcgtg ggctcttttg cggaacgtat
    24601 caagttcacg ccgctgatca tcttcgtggt gctgtggctg accctggtct atttcccgat
    24661 ggcgcacatg gtgtggtact gggctggccc ggacttctat tacaccaccg cctcgaccgc
    24721 cacgcctgac agcggctacc tcttcaccaa gggcgcgctg gactttgccg gcggcaccgt
    24781 ggtgcacatc aacgccggta tcgccggtct ggtgggctgc ctctacatcg gcaagcgtct
    24841 gggctatg
//
LOCUS       DASRYE010000023        10111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_177443,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000023 DASRYE010000000
VERSION     DASRYE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177443"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..511)
                     /locus_tag="VFF98_01345"
     CDS             complement(89..511)
                     /locus_tag="VFF98_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZU62300.1"
                     /translation="MQLHIIARGRIGRSPEAELVDRYVKRITWPCRITELPDRGGTVP
                     PAAGATRRVVLDERGRQISSEELAALLGRWRDEGVRECRFLIGAADGHAQDTRAEADL
                     LLGFGAMTWPHMLARAMLAEQLYRATAILAGHPYHRSG"
     gene            complement(594..944)
                     /gene="rsfS"
                     /locus_tag="VFF98_01350"
     CDS             complement(594..944)
                     /gene="rsfS"
                     /locus_tag="VFF98_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZU62301.1"
                     /translation="MPEAEPGSLHALVLQSLDDDQAQEIVSIPLAGKSAIADHMVIAS
                     GRSTRQVAAMAEKLAERVKHGGFGHARIEGLPAADWVVVDAGDVVVHLFRPEVRSFYN
                     LERMWTFGGSNAPA"
     gene            complement(1129..1830)
                     /locus_tag="VFF98_01355"
     CDS             complement(1129..1830)
                     /locus_tag="VFF98_01355"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZU62302.1"
                     /translation="MGNGFRSGRRAGPRIGLLGGSFNPAHHGHRHISLGALRALGLDE
                     VWWLVSPGNPLKEAAGMAPLAARFASARAAAARAPIRVSAIEREMGTRFTLDTLRALA
                     RRYPARQFIWLMGADNLAQFHRWRGWRQIARIMPVAVIARPGYDATALASPAMAWLRR
                     FRRPLASMAHRADGAHREGWSAPVLVLLAFDPDPRSASAVRLANPGWSATRPLPPGRD
                     DLTHRLISFAGAPGA"
     gene            complement(1842..3128)
                     /locus_tag="VFF98_01360"
     CDS             complement(1842..3128)
                     /locus_tag="VFF98_01360"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZU62303.1"
                     /translation="MSHTETLDSPATESADALVVRLAHAGRAAQAVLARLSPAEKSAA
                     LVAAAEALRRAAPAILAANAEDMANGAARGLTAALLDRLRLDDKRLAGMADAVENVAR
                     LPNPVGQVIGTDTRPNGLVLSRVRVPIGLIGIIYESRPNVTADAAALCLASGNAVLLR
                     GGTEAVHSNRAIHAALVAGLAAHGVPAAAVQLLPTQDRAAVGAMLTAAGLIDMIVPRG
                     GKSLVERVQADARVPVLAHLDGICHTYVHAAADVEMAKAVTLNAKMRRTGICGSMETL
                     LLDAAFPANQAVVAGLIEAGCALRGDARARALDPRIQPASAADWDTEYLDAILSVAVV
                     DGLEAALTHIAAHSSHHTDAILTADEAVAEEFLNRVDSAIVMVNASTQFADGGEFGLG
                     AEIGIATGRLHARGPVALEGLTTYKWQVRGTGQLRP"
     gene            3341..4513
                     /locus_tag="VFF98_01365"
     CDS             3341..4513
                     /locus_tag="VFF98_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZU62304.1"
                     /translation="MDRISARAALVRLRAHFPEREFLMRSEGRVRFIRLSTRLQMQLC
                     AGVAALALGWVGTLATVALLRLHDSFDQQSLLNRAARVESAEKRVGAYRRNLDSVAAD
                     LRRRQDFIEKMVEAHVGELPADHRAGETVSDSSTEAGKAVSKISAAVPEAAPLARMEA
                     RQLAFAEQLTRWADRRATGETDRLRRLGLNPAMLLAAQNNGEAEGGPLLRLATESDGG
                     LDPRFARLGASLARMSALDAGLTRVPQVLPARMDYISSGFGYRADPFTHAGAFHPGLD
                     FRGPTGAPIYAAAPGTVSFAGQRSGYGNCVEVTHGPGLVTRYAHMSAFRARVGQQVVA
                     GQQIGAIGSTGRSTGPHLHFEVRINDRPVNPRPFLEVRLHVPEKIGAGIVAGAADD"
     gene            4461..4883
                     /locus_tag="VFF98_01370"
     CDS             4461..4883
                     /locus_tag="VFF98_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZU62305.1"
                     /translation="MFQKKSAPASWPARQTTDPVATGFSMLGADVAIRGDISASADLH
                     IDGRVEGDIACAALVQGEASEITGAIAAASARLAGTVRGSISVSQLTIQKSARIHGDV
                     HYDQLTIEQGAQVEGRFAQREPAKELPGKGAVPLLAAD"
     gene            complement(4973..5674)
                     /locus_tag="VFF98_01375"
     CDS             complement(4973..5674)
                     /locus_tag="VFF98_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HZU62306.1"
                     /translation="MTQPTPLMPHATASWLVDNTALTFEQIAEFCGLHMLEVQAMADD
                     LASAKYKGRDPVRAGELTNAEIEKGQANPAYRLRMFKAPVTVSRTKGPRYTPVSKRQD
                     KPDGIAWILRHHPELSDAQVSKLIGTTRNTIAAIRDRSHWNIGNINPKDPVTLGLCSQ
                     RELDGMVAKAAKGRSAEDDEADVQRLGSDRDALIEELRAAREAQARAAANAAQEAEAA
                     AWLLAKRAAEAENGE"
     gene            complement(5841..6470)
                     /locus_tag="VFF98_01380"
     CDS             complement(5841..6470)
                     /locus_tag="VFF98_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU62307.1"
                     /translation="MSAALILHEDPISANCYKIRLTAALLGLPLARRAYDIRKGDTRK
                     PEFLRDISPVGSIPVLQVGEGADARFLPESNAACWFLAEGSRLVPQDRFERAETLRWM
                     FFEQYSHEPNVATLRFWLAFVGEANLSAERRGQIALKRQYGEEALALMDRHLAGRDWF
                     VGNAPSLADLVLYAYTHVAEEGGFALAGLGHLRGWLGRVAGLDGYVGMD"
     gene            complement(6467..7474)
                     /locus_tag="VFF98_01385"
     CDS             complement(6467..7474)
                     /locus_tag="VFF98_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZU62308.1"
                     /translation="MIDVLPETMRAVALDGAGGPEVLGIAALALPRPAAGQVLVKVAY
                     AGVNRPDIVQRQGHYPAPAGHSPLLGLEISGHVVALGEGVSEALLGQPVCALVNGGGY
                     AEYCLAEAGHCLPVPAGLPLAEAAAMPETLFTVWHNVFERGWACEGEHLLVHGGTSGI
                     GTMAIQLGKLFGLTVIVTCGGAEKCARALEIGAAHAIDYRAADFVEEVRRITGGRGVE
                     LVLDMVAGDYVARNLKCLADDGRHVTIAVQGGARAEVPMWQVMARRLTLTGSTLRARS
                     NEFKTLVAQELAANVWPLVAAGQLRPVMDEVFPLAEVAAAHARMEQGAHIGKIVLAVA
                     G"
     gene            7594..7806
                     /locus_tag="VFF98_01390"
     CDS             7594..7806
                     /locus_tag="VFF98_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 family protein"
                     /protein_id="HZU62309.1"
                     /translation="MEDEDILPRSGARRDAASLLSGEALDAYSLEELDERIRLLAAET
                     ERVRAHRIKAEAHRRAAEALFRPNAG"
     gene            7960..>10111
                     /gene="clpA"
                     /locus_tag="VFF98_01395"
     CDS             7960..>10111
                     /gene="clpA"
                     /locus_tag="VFF98_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZU62310.1"
                     /translation="MPSFAPSLEKTLHSALGHASERSHEYATLEHLLLALIDDPDAAQ
                     VMQACGVDLSELGDAVRQYLDQEYQSLKTKEKGDPAPTAGFQRVIQRAILHVQSSGKD
                     TVTGANVLVALFSERDSYAVYFLQQQDMSRLDAVSYISHGIGKGGRRIEDKTPKGAET
                     EAPHAEEKPETKSAGKKDSALDQFCVNLNDKALLGKVDPLIGRGPEVDRTIQILCRRS
                     KNNPLYVGDPGVGKTAIAEGLARKIVEGEVPEVLANAVIYSLDMGSLLAGTRYRGDFE
                     ERLKQVVSELEKMPHAILFIDEIHTVIGAGATSGGAMDASNLLKPALSGGTIRCIGST
                     TYKEFRNHFEKDRALLRRFQKIDVNEPSVEDTIKILKGLRTAFEEHHKVKYTPEAIKT
                     AVELSARYINDRKLPDKAIDVIDEVGAMQMLVPPSKRKKTITAREIEAVIATMARIPP
                     KSVSSDDKKVLEHLERDLKRVVFGQDKAIEVLSAAMKLSRAGLRDPDKPIGSFLFSGP
                     TGVGKTEVARRLAEIMGIPLQRFDMSEYMERHSVSRLIGAPPGYVGFDQGGLLTDAID
                     QQPHCVLLLDEIEKAHPDLFNILLQVMDNGRLTDHHGKTVDFRNVVLIMTTNAGASDM
                     ARQGIGFGDVSKQDAGDEAVKNLFTPEFRNRLDAIVPFAYLPTTVVSRVVDKFILQLE
                     LQLADQNVHIQFDAEARAWLGERGYDKLYGARPMA"
BASE COUNT         1676 a         3447 c         3416 g         1572 t
ORIGIN      
        1 aggcgccgcc tggggcgtgg ccgcctgcag ccagccgccc gccagcatca gcgccagcgc
       61 cgccggccag gtgccgcgcc gcaccatctc agccgctccg gtgatagggg tggccggcca
      121 ggatcgccgt ggcgcgatag agctgctctg ccagcatggc gcgggccagc atgtgcggcc
      181 aggtcatcgc gccaaagccc agcagcagat cggcttcggc gcgggtatcc tgcgcatggc
      241 catcggccgc gccgatcagg aagcggcatt cgcgcacgcc ctcgtcgcgc cagcggccca
      301 gcagggcggc cagctcttcc gaggagatct gccggccgcg ctcgtccagc accacgcggc
      361 gggtggcgcc ggcggcaggc ggcaccgtgc cgccgcgatc gggcagttcg gtgatgcggc
      421 agggccaggt gatgcgcttc acatagcgat cgaccagctc tgcctcgggc gaacggccta
      481 tccgcccgcg cgcgatgata tgcagctgca tcttgcccct tcaaccggca tgccggatcc
      541 gcccggggcg gccggcccaa gcggggcagc cccctgccct gcgtggcaga acctcaggcc
      601 ggggcgttgc tgccgccgaa ggtccacatc cgctcgaggt tgtagaagct gcgcacttcc
      661 gggcggaaga gatgcaccac cacatcgccg gcatcaacca ccacccagtc ggccgcgggc
      721 aggccctcga tccgggcgtg gccaaagccg ccgtgcttca cgcgctcggc cagcttttcg
      781 gccatggcgg ccacctggcg ggtggaacgg cccgaggcga tcaccatgtg atcggcaatg
      841 gcgcttttgc cggcaagcgg gatggagacg atctcctgcg cctgatcatc atccagcgac
      901 tggaggacga gggcgtggag cgagcccggc tcggcttcag gcaggggctg ctggccaaga
      961 gctgtgggcg ggatcgggct ggcggcggaa ggcgccgcag aaggtgtggt gtggttcatg
     1021 tagtctggat gcactcctct ctcatggggc gattgtcaag cggtagcgcg tggcgcggtg
     1081 ggcgggctgt ctcgccgtgg ggcaagcgca caggggccgc cgccggcatc atgccccggg
     1141 cgctccggca aaagatatga ggcgatgggt caaatcatcg cgcccgggcg gcagggggcg
     1201 ggttgcgctc catcccggat tggccagacg gacagcgctg gccgaacgcg gatcgggatc
     1261 aaaggccaga agcaccagaa ccggcgcgct ccatccttcc cggtgggccc catcggcccg
     1321 gtgagccatg ctggccagcg gccgccggaa acggcgcagc caggccatcg ccggactggc
     1381 gagggcagtg gcatcatatc ccggccgcgc tatcaccgca acgggcatga tgcgggcgat
     1441 ctgccgccag ccccgccagc ggtggaactg ggccagatta tccgccccca tcaaccagat
     1501 gaactgccgc gccggatagc gccgggccag cgcgcgcagg gtatcgagcg tgaagcgcgt
     1561 gcccatttcc cgctcgatcg cgctgacgcg gatgggcgcg cgggccgccg ctgcccgggc
     1621 gctggcaaag cgggcggcca gcggcgccat gccggcggcc tccttcagcg ggttgccggg
     1681 cgagaccagc caccacacct catccagccc cagcgcgcgc agggcgccca ggctgatgtg
     1741 gcggtggcca tgatgggccg ggttgaaact gccgcccagc aggccgatgc gcggccccgc
     1801 ccgccggccg gatcggaaac cattgcccat gccgccagcg ctcaggggcg caattgcccc
     1861 gtgccgcgca cctgccactt gtaggtcgtc agcccttcca gcgccaccgg gccgcgggca
     1921 tggaggcggc cggtggcaat gccgatctcg gcgcccaggc caaattcgcc gccatcggca
     1981 aattgcgtgc tggcgttgac catgacgatc gccgaatcga cccggttgag gaactcttcg
     2041 gccaccgcct catcggcagt gaggatcgca tcggtgtggt ggctggaatg ggcggcgata
     2101 tgagtcagcg cggcttccag cccatccacc acggcaacag agaggatggc atcgagatat
     2161 tccgtatccc aatccgccgc gctggccggc tggatccgcg gatcgagcgc gcgggcacgg
     2221 gcatcaccgc gcagggcgca gccggcctcg atcagccctg ccaccaccgc ctgattggcc
     2281 gggaaggcgg catcgagcag cagcgtttcc atcgagccgc aaatgccggt gcgccgcatc
     2341 ttggcgttga gcgtgaccgc cttggccatc tccacatcgg cggcggcgtg cacataggtg
     2401 tggcagatgc catcgagatg ggccagcacc ggcacccggg catcggcctg cacgcgctcc
     2461 accaggcttt tgccaccgcg cggcacgatc atgtcgatca ggccggcggc cgtcagcatg
     2521 gcgccaacgg cggcgcggtc ctgcgtgggc agcagctgca cggcggcggc cggcacgcca
     2581 tgggctgcca gccccgccac cagcgcggca tggatcgccc ggttggaatg caccgcttcc
     2641 gtgccgccgc gcagcagcac cgcattgccc gaggccaggc acagggcggc ggcatcggcc
     2701 gtcacattcg gccggctttc gtagatgatg ccgatcaggc cgatgggcac ccgcacgcgg
     2761 ctgagcacca ggccattggg ccgcgtatcc gtgccgatca cctggccgac aggattgggc
     2821 aggcgcgcca cattctccac cgcatcggcc atgccggcca gccgcttgtc gtccagccgc
     2881 agccggtcga gcagggcggc ggtgaggccg cgggccgcgc cattggccat atcctcggca
     2941 ttggcggcaa ggatggctgg cgcggcgcgg cgcagggctt cagcggcggc caccagcgcg
     3001 gccgatttct ccgccggcga caggcgggcc agcacggctt gcgcggcacg gccggcatgg
     3061 gcaaggcgca ccaccagcgc atcggcgctt tcggtggcgg gggaatcgag cgtttcggtg
     3121 tgcgacataa tcttgccgct ctagcaccct gatgcggccc tgtcaggccc aagcggcagg
     3181 aagggcgggc agaggcgcgg cctagcgctc gattcatcgt gcacgagggg cattctggcg
     3241 caatttgacg cagcgccgcc gatggcggat tcgcctgggg gcggcccggc tgttatgggg
     3301 ccggcccgga tcgtaaccaa gtgtttgaaa cggggccact ttggacagga tttcggcaag
     3361 ggccgcgctg gttcgcctgc gcgcccattt tcccgagcgc gagtttctca tgcgctcgga
     3421 agggcgggtg cgctttatcc gcctttccac ccggctgcag atgcagctgt gcgccggcgt
     3481 tgcggccctg gctctgggct gggtgggcac gctggccacg gtggcgctgc tgcgcctgca
     3541 cgacagtttc gaccagcaaa gcctgctgaa ccgcgccgcc cgcgtggaaa gcgccgaaaa
     3601 gcgcgtgggc gcctatcgcc gcaacctcga ttccgtggcg gcggatttgc gccgccggca
     3661 ggatttcatc gagaagatgg tggaggccca tgtgggcgaa ctgcctgcgg accatcgcgc
     3721 cggcgaaacc gtgtccgatt ccagcaccga ggcgggcaag gcggtctcca agatcagcgc
     3781 cgccgtgccc gaggcagccc cccttgcccg catggaagcg cgccaactgg cctttgccga
     3841 acagctgacc cgctgggccg accggcgcgc caccggtgag accgaccgct tgcgcaggct
     3901 gggcctcaac ccggccatgc tgctggccgc gcagaacaat ggcgaggccg agggcggccc
     3961 gctgctgcgc ctggccaccg agagcgatgg cggccttgat ccgcgctttg cccggctggg
     4021 cgccagcctg gcgcggatga gcgcgctgga tgccggactg acccgcgtgc cccaggtgct
     4081 gcccgcgcgg atggactata tctcctcggg ctttggctat cgcgccgatc cgttcaccca
     4141 tgccggcgcc tttcaccccg gccttgattt tcgcgggccc acaggcgcgc cgatctatgc
     4201 cgcggcgccc ggcacggtca gctttgccgg gcagcgttcg ggctatggca attgcgtgga
     4261 agtgacgcat gggccgggcc tggtcacgcg ctatgcgcat atgtcggcct ttcgcgcgcg
     4321 ggtgggccag caggtggtgg ccggccagca gatcggcgcg atcggctcca ccggccgttc
     4381 caccgggccc catttgcatt tcgaggtgcg gatcaacgac cgccccgtca atccccgtcc
     4441 cttcctggag gttcgcttgc atgttccaga aaaaatcggc gccggcatcg tggccggcgc
     4501 ggcagacgac tgaccctgtg gccaccggct tttccatgct gggcgccgat gtggcgatcc
     4561 gcggcgacat ttcggccagt gctgacctcc atatcgacgg ccgggtggag ggcgacattg
     4621 cctgcgccgc gctggtgcag ggcgaggcca gcgagatcac cggggcgatt gccgctgcct
     4681 cggcgcggct ggccggcacg gtgcgcggca gcatctcggt cagccagctg accatccaga
     4741 aaagcgcgcg catccatggc gatgtgcatt acgaccagct gaccatcgag cagggcgcgc
     4801 aggtggaagg ccgctttgcc cagcgcgagc cggcaaagga gctgcccggc aagggcgcgg
     4861 tgccgctgct ggcggcggat taaggcgccg cgcaaggcac cctgccacac acgaaaaggc
     4921 cggccctccc ccaacggaag gccggccttt tgcctgcata aaatgcagaa aattattcgc
     4981 cgttttcagc ctcggcggcg cgcttggcca gcagccaggc ggcggcttcg gcttcctgcg
     5041 cggcattggc ggcagcgcgg gcctgcgcct cgcgggcggc gcgcagttcc tcgatcagcg
     5101 catcgcggtc gctgcccagg cgctgcacat cggcctcgtc atcctcggca ctgcggccct
     5161 tggcggcctt ggccaccatg ccatccagct cacgctgcga gcacaggccc agcgtcaccg
     5221 gatccttcgg gttgatgttg ccgatgttcc agtggctgcg atcgcggatc gcggcgatgg
     5281 tgttgcgggt ggtgccgatc aacttggaga cctgcgcatc cgacagttcg ggatggtggc
     5341 gcaggatcca ggcgatgcca tcgggcttgt cctgccgctt cgacaccggc gtgtagcgcg
     5401 ggcccttggt gcggctgacg gtgaccggcg ccttgaacat cctgagtcgg taagcggggt
     5461 tggcctggcc cttctcgatc tcggcattgg tcagctcgcc cgcgcgcaca gggtcgcggc
     5521 ccttgtattt ggcgctggcg aggtcgtcgg ccatcgcctg cacctcaagc atgtgcaggc
     5581 cgcagaattc ggcgatctgc tcgaaggtga gcgcggtgtt gtcaacaagc cacgatgccg
     5641 tcgcatgggg catcagcggg gtgggctggg tcacgagagc tctccgtcgc ggccgggaaa
     5701 ttgcggcccg aaaagggact tgaaacaaga aggccgcccc ttcacggagc ggcccttcga
     5761 ctgcgcgtga taagcgattt gccccggccc tgcaagaaaa aggcgcgcat tgcctgtaaa
     5821 tcgcttcgaa atcggtgccg tcaatccatg ccgacatagc catcaagccc ggccacccgg
     5881 cccagccagc cgcgcagatg gccaagcccg gccagcgcaa agccgccctc ctcggccaca
     5941 tgggtatagg cataaagcac gagatcggcg aggctggggg cattgcccac aaaccagtcg
     6001 cgcccggcca gatggcggtc catcagcgcc agcgcttcct cgccatactg gcgcttcagg
     6061 gcgatctgcc cgcgccgctc ggccgacaga ttcgcctcgc ccacaaaggc cagccagaag
     6121 cgcaaggtgg ccacgttcgg ctcgtggctg tattgttcga agaacatcca gcgcaaggtt
     6181 tcggcccgct cgaaccggtc ttgcggaacc agccggctgc cctcggccag aaaccagcag
     6241 gccgcgttgc tttcgggcag gaagcgggca tcggccccct cgcccacctg cagcacggga
     6301 atgctgccaa ccgggctgat atcgcgcagg aattccggct tgcgggtatc gcccttgcgg
     6361 atatcatagg cgcggcgcgc cagcggcagg cccagcagcg ccgcggtcag gcggatcttg
     6421 tagcaattgg cggaaatcgg gtcttcgtgc aggatcagcg cggcgctcat ccggccaccg
     6481 ccagcacgat cttgccgata tgggcgccct gttccatccg cgcatgggcg gcggccactt
     6541 cggccagcgg gaacacctcg tccatcaccg ggcgcaattg cccggcggcc accagcggcc
     6601 acacgttggc ggccagttcc tgggccacca gcgtcttgaa ttcattgctg cgcgcccgca
     6661 aggtagagcc ggtcagcgtc agccttcggg ccatcacctg ccacatcggc acttcggccc
     6721 tggcgccgcc ctgcacagcg atcgtcacat ggcggccatc atcggccagg cactttaggt
     6781 tgcgcgccac ataatcgccc gccaccatat cgagcaccag ttccaccccg cgcccgccgg
     6841 tgatgcggcg cacttcctcg acgaaatcgg ccgcgcgata gtcgatcgca tgggccgcgc
     6901 caatctccag cgcgcgggcg catttttccg cccccccgca ggtgacgatg accgtgaggc
     6961 cgaacagctt gcccagctgg atcgccattg tgccgatgcc gctggtgccg ccatgcacca
     7021 gcagatgctc gccctcgcag gcccagccgc gttcgaacac attgtgccac acggtgaaca
     7081 gcgtttcggg catggcggcc gcctcggcca gcggcaggcc ggcgggcacc ggcaggcagt
     7141 ggcccgcttc ggcgaggcaa tattcggcat agccgccgcc gttcaccagc gcgcacaccg
     7201 gctggcccag cagcgcttcc gacaccccct cgcccagcgc caccacatgg ccggaaattt
     7261 ccagccccag cagcggcgaa tggccggccg gcgcgggata atgcccctgg cgctggacga
     7321 tgtcgggccg gttcacgccg gcataggcca ccttcaccag cacctgtccc gccgcgggac
     7381 ggggcagcgc cagagcggca atgcccaaca cctcgggccc gccggcgcca tcaagggcca
     7441 cggcccgcat ggtttcgggc aatacgtcga tcatcagttc gggctcgccc ccggccatct
     7501 gtgcctcgat tgtttccggc tgatacgcct gttacccggt gattagcctt tgagatggtt
     7561 gacagcaagc gcggcgaggg ggatgctgcc cgaatggagg acgaagacat ccttccgcgc
     7621 tccggcgccc gccgggatgc cgccagcctg ctctccggcg aggcccttga tgcctactcg
     7681 ctcgaagagc tggacgagcg catccgcctg ctggctgccg aaaccgaaag ggtgcgtgcc
     7741 catcggatca aggccgaggc ccaccgccgc gccgccgagg cgctgttcag gccaaacgcc
     7801 ggatgagccg cggggccctt ggttcttccc cccctcgtaa tcctgcaagg ggggcccata
     7861 taggcctggt taacctcact cgtcgcatat cttcctctgg cctccacctg gggtcgtgcc
     7921 ccccatctgg catggcgtcc ctcagcgaaa gagccctaga tgcccagttt tgccccaagc
     7981 cttgagaaga ccctgcattc cgcccttggc cacgcttccg agcgcagcca cgaatatgcc
     8041 acgctcgaac atctgctgct ggcgctgatc gacgatcccg atgcggcaca ggtgatgcag
     8101 gcctgcgggg tggatctttc cgaactgggc gatgccgtgc gccagtatct cgaccaggaa
     8161 taccagagcc tgaaaaccaa ggaaaagggc gaccctgccc ccaccgccgg tttccagcgg
     8221 gtgatccagc gcgccatcct gcacgtgcaa tcctcgggca aggatacggt gacgggcgcc
     8281 aatgtgctgg tggcgctgtt ctcggaacgg gattcctatg ccgtctattt cctccagcag
     8341 caggacatga gccggctgga tgcggtgagc tatatcagcc acggcatcgg caagggcggc
     8401 cgccgcatcg aggacaagac ccccaagggc gccgagaccg aggcccccca tgccgaggaa
     8461 aagcccgaga cgaagagcgc gggcaagaag gattccgcgc tcgaccagtt ctgcgtcaac
     8521 ctcaacgaca aggcgctgct gggcaaggtg gatccgctga tcggccgcgg gccggaggtg
     8581 gaccgcacga tccagatcct gtgccgccgt tccaagaaca atccgctgta tgtgggcgac
     8641 ccgggcgtgg gcaagacggc gatcgcggaa ggccttgcgc gcaagatcgt ggaaggcgaa
     8701 gtgcccgagg tgctggccaa tgcggtgatc tattcgctcg acatgggcag cctgctggcg
     8761 ggcacgcgct atcgcggcga ttttgaagag cggctgaagc aggttgtctc cgaactggag
     8821 aagatgcccc atgccatcct cttcatcgat gaaatccaca cggtgatcgg cgccggcgcc
     8881 accagcggcg gggcgatgga tgcttccaat ctgctcaagc cggcgctgag cggcggcacc
     8941 atccgctgca tcggctcgac cacctacaag gaattccgca accacttcga gaaggaccgc
     9001 gcgctgctgc gccggttcca gaagatcgac gtgaacgagc cttcggtgga agacacgatc
     9061 aagatcctca agggcctgcg caccgccttc gaggaacacc acaaggtgaa atacaccccc
     9121 gaggcgatca agaccgcggt ggaactgtcg gcccgctaca tcaacgaccg caagctgccc
     9181 gacaaggcga tcgacgtgat cgacgaggtg ggcgcgatgc agatgctggt gccgccatcg
     9241 aagcgcaaga agacgatcac cgcgcgcgag atcgaggcgg tgatcgccac catggcgcgc
     9301 atcccgccca agagcgtgtc cagcgacgac aagaaggtgc tggaacatct ggagcgcgac
     9361 ctgaagcggg tggtgttcgg gcaggacaag gcgatcgagg tgctgtcggc cgcgatgaag
     9421 ctctcccgcg cggggctgcg cgatcccgac aagccgatcg gcagcttcct gttctccggc
     9481 cccacgggcg ttggcaagac ggaagtggcc cggcggctgg ccgagatcat gggcattccg
     9541 ctgcagcgct tcgacatgtc ggaatatatg gagcggcatt cggtctcgcg gctgattggc
     9601 gcgcctccgg gctatgtcgg cttcgatcag ggcggtttgc tgaccgatgc gatcgaccag
     9661 cagccccatt gcgtgctgct gctcgacgag atcgagaagg cgcatccgga tctgttcaac
     9721 atcctgctgc aggtgatgga caacggccgg ttgaccgatc accacggcaa gacggtggat
     9781 ttccgcaatg tggtgctgat catgaccacc aatgccggcg cttccgacat ggcgcgccag
     9841 ggcatcggct ttggcgatgt gtccaagcag gatgccggcg atgaggcggt gaagaacctg
     9901 ttcacgccgg aattccgcaa ccgcctcgat gcgatcgtgc cctttgccta tctgccgacc
     9961 acggtcgtca gccgggtggt ggacaagttc atcctgcagc tggaactgca gctggccgac
    10021 cagaacgtgc acatccagtt cgatgcggaa gcgcgcgcct ggctgggcga gcgtggctac
    10081 gacaagctct atggcgcgcg gccgatggcc c
//
LOCUS       DASRYE010000024         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_231077,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000024 DASRYE010000000
VERSION     DASRYE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231077"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..824
                     /locus_tag="VFF98_01400"
     CDS             24..824
                     /locus_tag="VFF98_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU62311.1"
                     /translation="MDLDTRILIVDDDEGIRSLISEFLGKHGFATATAADPIEMREML
                     GREVYDLVVLDVMMPREDGLTALRQFGPDAPPVIMLSAVGSDIDRIVGLEMGADDYLA
                     KPCNPRELLARIRTVLRRYGKGAPHPANDDAPIAPPKPRSDAESEPNSGDNLCFGGWR
                     MDLGLRLLFDPEGRLITLSDGEFRLLKAFAEHPRRVLTRDQLLEWSRGEESEHYDRAI
                     DVQLSRLRRKLAVGRGSEDIIRTVRNEGYLFVPAVNREQRDGEVAPPA"
     gene            complement(860..2284)
                     /locus_tag="VFF98_01405"
     CDS             complement(860..2284)
                     /locus_tag="VFF98_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU62312.1"
                     /translation="MKSAACRAKLALGAACLVALAGCKAVGPDYHLPQKAAINLPAAQ
                     GAFLGAAPAGADAKPLPPRWWHLFDAPVLDRLEEQALAANTDLRVAAANLKRAQEATG
                     MAESAHEPDFTASFAGERARLSGESFLLSEPVPVMNVGSGQIAVGYQLDLFGRIRRAV
                     EEARASEQAVAAVQDAVKASIAADVARSYVMACAAGEDIALTDEAIAVEKRRLDVDER
                     LQAGGRLTPLELAASRARLAQVEAVRPAHVARQRAALFRLAYLLGKPPAEYPREVESC
                     EEIPSASGIMPVGDGASLLARRPDVRASERELAAATAQIGVATAAMYPDIRLGAALGS
                     FGKLGDFGTAPANFWNAGGMIDWAFPTHGARARVRAANAGADAALARFDGVVLAALRE
                     TETALSTYAQDLNRQHALAEALHQNELATADIERLRRAGRSPLSAHLGAEQGTISAKA
                     AVSEGKARLANDQVDLFLALGGGW"
     gene            complement(2281..3246)
                     /locus_tag="VFF98_01410"
     CDS             complement(2281..3246)
                     /locus_tag="VFF98_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU62313.1"
                     /translation="MTKLPPLADTLKPAIRLAVTGVVVVAAGFAIKALWDRYEVDPWT
                     RDGRVRADIVQIAPDVPGLVTAVYVVNDQSVKKGDPIFQVDPARYRLALSQAQTTIEQ
                     TRAAITSASAALAEARREAARNHQLGELVPLETTQQSDAKVEADAATLAAAKAAADAA
                     LVARDTAKLNLDRTLVRAPYDGVISEAYVKVGDYVSPGKMVIALFDRATLRIEGYFEE
                     TKLAGVHLGQKATARLMGDGRLVHGHVISIAAGIEDHDRQASANMVPNINPSFSWVRL
                     AQRVPVRIAIDDAPGDLALVAGRTVTVSLERDEQSRRQAAAGARK"
     gene            complement(3243..3464)
                     /locus_tag="VFF98_01415"
     CDS             complement(3243..3464)
                     /locus_tag="VFF98_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HZU62314.1"
                     /translation="MIAEFNLNGVYFASIPITSLLALVATLVLHRLLALVGAYRWIWH
                     AALFDAALFICLWALLVTTSLDHILGKIA"
     gene            complement(3461..>3862)
                     /locus_tag="VFF98_01420"
     CDS             complement(3461..>3862)
                     /locus_tag="VFF98_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZU62315.1"
                     /translation="LTPRLALADQGSPGRRDAEEGLRGLRIALNLAVLQNLRTQMERS
                     PRAAISRVLKTVAAHFGALPPVRRPPGAPAALLEQIDLALGRLAISQEHHRAEAIATL
                     VALRRNLFPQASASTPLPAPPPPPPADGQTA"
BASE COUNT          597 a         1301 c         1375 g          589 t
ORIGIN      
        1 cgatgaaaac gagccgagcc acgatggatc tggatacacg catactgatt gtcgacgacg
       61 atgagggcat tcgcagcctg atatcggaat ttctgggcaa gcatggcttt gccacggcca
      121 ccgcggccga tccgatcgaa atgcgcgaga tgctgggccg cgaggtgtac gatctggtcg
      181 tgctcgacgt gatgatgccc cgggaagacg ggttgaccgc gctgcgtcag tttggcccgg
      241 atgctccgcc ggtgatcatg ctgagcgcgg tgggcagcga cattgaccgc atcgttggcc
      301 tggaaatggg cgccgacgat tatctcgcca agccctgcaa cccgcgcgaa ctgctggcgc
      361 gcatccgcac cgtgctgcgc cgctatggca agggcgcgcc ccatccggcc aatgatgatg
      421 cccccatcgc cccgccaaag ccgcgcagcg atgccgaaag cgagcccaat tcgggcgaca
      481 acctgtgttt tggcggctgg cggatggatc tgggcctcag gctgctgttc gatccggaag
      541 ggcggctgat caccctttcg gatggcgaat tccgcctgct gaaggccttt gccgaacatc
      601 cgcgccgggt gctcacgcgt gatcagctgc tggaatggtc acgcggggaa gagagcgagc
      661 attacgaccg ggcgatcgat gtgcagctct cgcgcctgcg ccgcaagctg gccgtgggcc
      721 gcggcagcga ggatatcatc cgcaccgtgc gcaacgaggg ctatctcttc gtgcccgccg
      781 tcaaccgcga gcagcgcgat ggcgaggtgg cgccgcccgc ctgattttgt gtgtggaggg
      841 gaaggcgcgc gccttcccct taccagccgc cgcccagcgc gaggaacaga tcgacctgat
      901 cgttcgccag ccgggccttg ccttccgaca ccgccgcctt ggccgagatc gtgccctgtt
      961 ccgcgcccag atgggccgaa agcgggctgc ggccggcgcg gcgcaggcgc tcgatatcgg
     1021 cggtggccag ctcgttctgg tgcagggcct cggccagcgc gtgctggcgg ttgagatcct
     1081 gcgcataggt ggaaagggcg gtttccgtct cgcgcagggc cgccagcacc acgccatcga
     1141 agcgggccag cgccgcatcg gccccggcat tggccgcgcg cacccgggcg cgggcgccat
     1201 gggtggggaa ggcccagtcg atcatgccgc cggcattcca gaaattggcg ggcgctgtgc
     1261 caaaatcgcc cagcttgccg aaggagccca gcgccgcgcc caggcggata tcgggataca
     1321 tagcggcggt ggccacgccg atctgggcgg tggcggcggc cagttcgcgc tcggaagcgc
     1381 gcacatcggg gcggcgggcc agcagcgatg cgccatcgcc caccggcatg atgccgctgg
     1441 ccgaggggat ctcctcgcag ctttccacct cgcgcggata ttcggccggc ggcttgccca
     1501 agagataggc caagcggaac agcgccgcgc gctggcgggc cacatgggcc gggcgcaccg
     1561 cttccacctg cgccagccgc gcgcgcgagg cggccagctc cagcggggtg aggcggccac
     1621 cggcctgcag gcgctcgtcc acatcgaggc ggcgcttttc caccgcgatc gcctcgtcgg
     1681 taagggcaat atcctcgccc gccgcgcagg ccatcacata gctgcgcgcc acatcggcgg
     1741 cgatgctggc cttcaccgca tcctgcacgg cggccacggc ctgttcgctg gcgcgcgcct
     1801 cttccaccgc ccggcggatg cggccgaaca gatcgagctg atagcccacc gcgatctgcc
     1861 cgctgcccac attcatcacc ggcacaggct ctgaaagcag gaaggattcg cccgaaagcc
     1921 gcgcccgttc gcccgcgaaa gaggcggtga aatcgggctc atgcgcgctt tcggccatgc
     1981 cggtggcttc ctgcgcgcgc ttgaggttgg ccgcggccac gcgcagatcg gtgttggcag
     2041 ccagcgcctg ctcttccagc cggtcgagca cgggggcatc gaacaggtgc caccagcgcg
     2101 gcggcagtgg cttggcatcg gcgccggcgg gcgcggcgcc caggaaggcc ccttgcgcgg
     2161 ccggcaggtt gatggcggcc ttctgcggca ggtggtaatc cggccccacc gccttgcagc
     2221 cggccagcgc cacgaggcag gccgccccca gcgccagctt tgcccgacag gccgcgctct
     2281 tcacttgcgg gctccggcgg cggcctggcg gcggctctgc tcgtcgcgct ccagcgagac
     2341 ggtgacggtg cggccggcca ccagcgccag atcgccgggg gcatcgtcga tggcaatgcg
     2401 caccggcacg cgctgggcca ggcgcaccca gctgaagctg gggttgatgt tgggcaccat
     2461 gttggcgctg gcctggcggt cgtggtcctc gatgccggcg gcaatggaga tgacatggcc
     2521 atgcaccagc cgcccatcgc ccatcagccg ggcggtggcc ttctggccaa gatgcacgcc
     2581 ggccagcttg gtttcctcga aatagccctc gatgcgcagg gtcgcgcggt cgaacagggc
     2641 gatcaccatc ttgcccgggc tgacgtaatc gcccaccttc acataggctt cggagatgac
     2701 gccatcatag ggcgcgcgca ccagcgtgcg atcgaggttc agtttggcgg tatcgcgcgc
     2761 caccagcgcg gcatcggccg ctgccttggc ggccgccagc gttgccgcat cggcttccac
     2821 cttggcatcg ctttgctggg tggtttccag cggcaccagc tcgcccagct gatggttgcg
     2881 ggcggcctcg cggcgggctt cggccagcgc cgcactggcc gaggtgatcg ccgcgcgggt
     2941 ctgctcgata gtggtttggg cctgcgacag cgccagcctg tagcgcgccg gatcgacctg
     3001 gaagatggga tcgcccttct tcacgctctg gtcgttcacc acgtaaacgg cggtgacaag
     3061 gcccggcaca tccggcgcga tctgcacgat atcggcgcgc acgcgcccgt cgcgcgtcca
     3121 cgggtccacc tcgtagcggt cccacagggc cttgatggca aagccggcgg ccaccaccac
     3181 cacaccggtg acggcaagcc tgatggcagg tttcagcgtg tcggcaaggg ggggcagctt
     3241 ggtcatgcga tctttccgag aatgtgatcg agggaagtgg tcaccagcag cgcccacagg
     3301 cagatgaaca gcgccgcatc aaacagcgcg gcgtgccaga tccagcgata ggcccccacc
     3361 agcgccagca gccggtgcaa caccagcgtg gccaccagcg caagcagcga ggtgatgggg
     3421 atggaggcga aatagacgcc attgaggttg aattcggcga tcatgcggtc tggccgtcgg
     3481 ctggcggtgg agggggcggg gcgggcagcg gggtggaagc gctggcctgc gggaagaggt
     3541 tgcggcgcag cgccaccagc gtggcgatgg cctcggcgcg gtggtgctcc tggctgatgg
     3601 ccaggcggcc cagcgccagg tcgatctgct ccagcagcgc ggcgggcgcg ccgggggggc
     3661 ggcgcaccgg cggcagcgcg ccgaaatggg cggccacggt tttcagcacg cgggaaatgg
     3721 ccgcgcgggg gctgcgttcc atttgcgtgc gcaggttttg cagcacggcc agattcagcg
     3781 cgatgcgcag gccccgcagg ccttcctcgg catcgcgccg gccggggctg ccctgatcgg
     3841 ccagagccag gcgcggggtc aa
//
LOCUS       DASRYE010000025         9014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_213424,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000025 DASRYE010000000
VERSION     DASRYE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213424"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..809)
                     /locus_tag="VFF98_01425"
     CDS             complement(105..809)
                     /locus_tag="VFF98_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU62316.1"
                     /translation="MLEQIAYADRDTQLTGWLARPASAPRAAVLVLPTIANVTPNVER
                     KAAALAEAGYLALIADFYGAPVPSFEASRPLAEALRADPRHYRQRLHAGLATLCALPE
                     AAGLPRAAIGFCMGGQAALELARDGADIAAAVSFHGLLNTEVPAAPGAIKARLLVCHG
                     DADPLAPRAAVQGFWQEMDAAGANWHFHSYGSVLHGFTDPGSDARGVPALGFNASADR
                     QSWAAMHSLFDELFGA"
     gene            complement(802..2133)
                     /locus_tag="VFF98_01430"
     CDS             complement(802..2133)
                     /locus_tag="VFF98_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62317.1"
                     /translation="MSKIPPPLAPRSPLHIADYRRFWLARCCSVLATTGMVVILGYQL
                     YDVARNQYGYSIAQASFQLGVLGFVQFVPLFVLTPLAGVVADRFDRRHVGALSMSIDF
                     LVALALAICTARHWLSLPLLFALAVAHGTARVFVAPSIGAIAPKIVPAELLPRAIALN
                     STAMQVGTIFGPALAGLLFGHAQALPYAVAAMLLGAGAVSISLIRPLPALAANRQTHP
                     LRLVAEGFSYVRHNRFLLGCITLDLFAVLLAGATALLPVYARDILHVGPAGLGQMRAA
                     PAIGATVFALVLSVRPLKHNVGVKMLWAVAAFGLATVVFGLSRWYPLSLAALVVMGAA
                     DMISVFVRGSLVQLATPDDMRGRVGAISGLAISASNELGEMESGMAAALMGAPAAVVV
                     GGIGAVLVTLVWSLLFPELRLARTFSHFGPEGARREEPAAPEHPPQEVPHA"
     gene            complement(2198..2536)
                     /locus_tag="VFF98_01435"
     CDS             complement(2198..2536)
                     /locus_tag="VFF98_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU62318.1"
                     /translation="MSGGAQLSVTELRRSTRHPVSYAVAGEHRQIGEIQLLIANISAH
                     GFMATGQIQIGRGERVVIRLPHIGRIEAHLIWSTEDRAGFQFERIIRPDDFTQLIKTL
                     QPNPRLRRER"
     gene            complement(2709..3926)
                     /gene="tyrS"
                     /locus_tag="VFF98_01440"
     CDS             complement(2709..3926)
                     /gene="tyrS"
                     /locus_tag="VFF98_01440"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZU62319.1"
                     /translation="MSAYQSTLLRLLDERGYIHQLTDAHGLDKLAQTQVVPGYIGFDP
                     TAPSLHVGSLVQIMLLRRLQQAGHKPIVVMGGGTGKIGDPSFKDEARKLMTDEIIAGN
                     VASIRKIFDRFLTFGDGPADAIMVDNAEWLDKLGYIAFLREVGQHFSVNRMLSFDSVK
                     LRLDREQSLSFLEFNYMILQGYDFRELAQRYGCRLQMGGSDQWGNIVNGIELARRMDG
                     TEVYGVTTPLLTTADGAKMGKTMNGAVWLNEDALPAYDFWQYWRNTDDRDVGRFLKLF
                     TDLPLDEIARLEALEGSEINAAKVVLANEVTKLCRGEEAARAAEATAAATFAGGGLGA
                     DLPVLDVPAEGLAIIAALTAIGFTASNGEAKRKVAEGAVKLDDAVITDPALVVHEGKL
                     SLGKKKHGVLRRA"
     gene            4088..4576
                     /locus_tag="VFF98_01445"
     CDS             4088..4576
                     /locus_tag="VFF98_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HZU62320.1"
                     /translation="MRAVSVDWRADGDHFVLGWQVAGAAHLVVPPAVAPARADGLWQT
                     TCFELFLADAGTAYCEFNLSPSTRWAAYRFAAYRDGMAPQEMPQPPQITPGAAGGAVF
                     ACTVRLPAALLAGARAAGLCAVIEEAGGHLSYWALAHGPGQPDFHASACFALAIGAAE
                     AP"
     gene            4582..5784
                     /locus_tag="VFF98_01450"
     CDS             4582..5784
                     /locus_tag="VFF98_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZU62321.1"
                     /translation="MLFGIDRLLADPSLRAPLAGRRVALVAHPASVTAGLVHSLDALA
                     GLGDIRLASAFGPQHGLKGDKQDNMVETGDEIDAALGIPVFSLYGEVRRPTEAMMASA
                     DVFLFDLQDLGCRIYTFVTTLLYLLQAASGTGKAVWVLDRPNPAGRPVEGTLLLPGEE
                     SFVGAGPMPMRHGLTLGEMGHWFIRHFGLDVDYRVITMEGWQPHAAPGHGWPESRIWV
                     NPSPNAASLNMARAYAGTVMLEGATLSEGRGTTRPLEVLFGAPDVAAGAVLAEMRALA
                     PQWLAGCALRECWFTPTFHKHAGQLCSALMVHAEGAFYDHAAFRPWRLQALAFKAIRR
                     LYPDYPLWRDFPYEYEYDRLAIDVINGGPALRQWVDDAAAVPADLEALASHDEARWQE
                     ERAPFLLY"
     gene            complement(5827..7005)
                     /locus_tag="VFF98_01455"
     CDS             complement(5827..7005)
                     /locus_tag="VFF98_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZU62322.1"
                     /translation="MLTGFLEALRGAGIKAGLKEHLLLLEALEKQAIEPTPEAFYYLA
                     RAIYVKDESLIDRFDQVFARVFRGALGESELAAEIPEEWLRAVAEKFLSPEEMEKIQS
                     LGSWEEIMETLKQRLAEQQGRHQGGNKWIGTGGTSPFGNGGYNPEGIRIGGQSRHNRA
                     IKVWEKREFANFDATRELGTRNIKVALKRLRRFAREGAAEELDLDATIQGTARQGWLD
                     IRLRPERHNAVKVLLFLDVGGSMDPYIRLVEELFSAATTEFKNLEFFYFHNCLYEGVW
                     KDNRRRFAERTATWDILHTYGHDYKVVFVGDAAMSPYEISHPGGSVEHWNEEAGGVWL
                     ERVARTYPASVWLNPVPESRWAYMQSTRQVREIIGGRMFALTLEGLDGAMRELSRKRG
                     "
     gene            complement(7013..7459)
                     /locus_tag="VFF98_01460"
     CDS             complement(7013..7459)
                     /locus_tag="VFF98_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZU62323.1"
                     /translation="MSDHLQQGPHEGGCLCGQVRFSVDAAPLGARMCWCRDCQRIASG
                     SATVNVLFPAEAFSFTGEIATYHRSADSGNVVERGFCPECGAQIFSRPLDNPGQPIRL
                     RAGVLDNPNLIAPQAIIWTASAPSWVKFDPDLPAYPKAPPAAPHKI"
     gene            complement(7477..8334)
                     /locus_tag="VFF98_01465"
     CDS             complement(7477..8334)
                     /locus_tag="VFF98_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZU62324.1"
                     /translation="MAEASPRFDSTENYVATEDLKVAVNAAVLLRRPLLVKGEPGTGK
                     TVLAQEIARALDAPLIEWHVKSTTRAHQGLYEYDAVARLRDGQLGDPRVHDIANYIRR
                     GKLWEAFASERLPVLLIDEIDKADIEFPNDLLHELDRMAFDVYETGERIAARERPVVV
                     ITSNNEKELPDAFLRRCFFHYIKFPERETMLSIIDVHFPGIQKVLVHRALDMFYELRE
                     VPGLKKKPSTSELIDWLKLLLAEDMPLDVLASRDGAKAIPPLHGALLKNEADIMLFER
                     LAFMARRGG"
     gene            8564..>9014
                     /locus_tag="VFF98_01470"
     CDS             8564..>9014
                     /locus_tag="VFF98_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62325.1"
                     /translation="MKRGWSQQQLADLSGLSVRTIQRMEQGQPAALESYKALGAAFNV
                     DFAELQEQTVRDMTSDADKTELALAFAHVRAVRKFYFGLFTYVVVMALLAGINLATSP
                     HHLWFVFPLLGWGVGLALRATRVFGLLPWFGPEWEKAQVEKRLGRPLA"
BASE COUNT         1465 a         2966 c         3098 g         1485 t
ORIGIN      
        1 ccccgcctgc cacaccggcg cgcgcagctg cttctatcgc cggctggagg gcgatgggct
       61 ggtgccggtg gagggctgag ccaccccacc gggccagagg cggatcaggc gccgaacagc
      121 tcgtcgaaca ggctgtgcat ggccgcccag ctctgccggt cggcgctggc gttgaagccc
      181 agcgccggca cgccgcgcgc atcgctgccc ggatcggtga agccatgcag cacgctgcca
      241 tagctgtgga agtgccagtt ggcgccggcc gcgtccatct cctgccagaa gccctgcacc
      301 gccgcgcgcg gggccagcgg atcggcatcg ccatggcaca ccagcaggcg ggccttgatg
      361 gcgccggggg cggccggcac ttccgtgttc aaaagcccgt ggaagctgac ggccgcggcg
      421 atatcagcgc catcgcgcgc cagttccagc gctgcctggc cgcccatgca gaagccgatg
      481 gccgcgcgcg gcaggccggc cgcttccggc agggcgcaaa gggtggcaag gccggcatgc
      541 aggcgctggc gatagtggcg cggatcggcg cgcagcgcct ctgccagcgg gcgcgaggcc
      601 tcgaagctgg gcactggcgc gccatagaaa tcggcgatca gcgcaagata gccggcctcg
      661 gccaaggcgg cggccttgcg ctccacattg ggggtaacgt tggcgatggt gggcagcacc
      721 agcacggcgg cgcggggggc gctggccggg cgggccagcc agccggtcag ctgcgtgtcg
      781 cggtcggcat aggcgatctg ttcaagcatg gggcacctct tgcgggggat gttcgggggc
      841 ggcaggctcc tcgcgccggg cgccttcggg gccgaaatgg ctgaatgtgc gggccaggcg
      901 cagttcgggg aacagcaggc tccacaccag cgtcaccagc accgcgccaa tgccgcccac
      961 caccaccgct gccggcgcgc ccatgagggc ggcggccatg cccgattcca tctcgcccag
     1021 ctcgttggag gcggaaatcg ccaggcccga aatggcgccc acccggccgc gcatgtcatc
     1081 cggcgttgcc agctgcacca ggctgccgcg cacgaagacc gagatcatgt cggccgcgcc
     1141 catcaccacc agcgccgcca gcgacagcgg ataccagcgc gacaggccga agaccaccgt
     1201 ggccaggcca aaggcggcca ccgcccacag catcttcacc cccacattgt gcttgagcgg
     1261 ccgcaccgac agcaccagcg caaacaccgt ggcgccaatg gcaggggcgg cgcgcatctg
     1321 gccaagcccg gccggcccca catggaggat atcacgcgca tagaccggca gcagcgcggt
     1381 ggccccggcc agcagcacgg cgaacagatc cagcgtgatg cagcccagga ggaagcggtt
     1441 gtggcgcaca taggagaagc cctcggccac cagccgcagc gggtgcgtct gccggttggc
     1501 ggccagcgct ggcagcgggc ggatcaggct gatcgacacc gcgcccgccc ccagcagcat
     1561 cgccgccacc gcatagggca gcgcctgggc atggccgaac agcaggccgg ccagcgccgg
     1621 gccaaagatg gtgcccacct gcatggcggt ggaattgagg gcaatggcgc gcggcagcag
     1681 ctctgccggc acgatcttgg gagcgatggc gccgatcgag ggggccacga acacccgcgc
     1741 cgtgccatgc gccacggcca gcgcgaacag cagcggcagg ctcagccagt ggcgggcggt
     1801 gcagatggcc agcgccagcg ccaccagaaa atcgatgctc atcgagaggg cgccaacatg
     1861 gcgccggtcg aaccggtcgg ccaccacgcc ggccagcggc gtcagcacga acagcggcac
     1921 aaactgcaca aagcccagca cgcccagctg gaacgaggcc tgggcgatgg aatagccata
     1981 ctggttgcgg gccacatcgt agagctgata gcccagaatc actaccatgc cggtggtggc
     2041 cagcaccgaa cagcagcgcg ccagccagaa gcggcgataa tcggcgatgt gcagcggcga
     2101 gcgcggggcg agaggagggg ggatcttgct cacacagcgc catgtggcac tgcccgccgg
     2161 ccttgccaag cgcttttgcc gccggcccgg tggggtttca gcgttcgcgg cgcaggcggg
     2221 ggttgggctg cagggtcttg atcagctggg taaaatcatc cgggcggatg atccgctcga
     2281 actggaagcc ggcgcgatcc tcggtgctcc agataaggtg ggcctcgatc cggccgatat
     2341 ggggcaggcg gatcaccacc cgctcgccgc ggccgatctg gatctggccg gtggccatga
     2401 agccatgtgc cgagatattg gcgatcagca gctggatctc gcctatctgc cggtgctctc
     2461 ccgccacggc atagctgacc ggatggcgag tcgaccggcg caattcggtg accgaaagtt
     2521 gcgcgccgcc tgacatctga ttgtattccc ttgcttttga aaaccaagcc aagggattgt
     2581 ggcgcaattt tgcaaaggaa ttgtaaaggt tgggtaagaa atcgcggttt tccgcgcggc
     2641 ctgtggcagc tggcgcacgg ctggccgcag gcaagcggcg cagcggcgcg cttgcccgcg
     2701 gcgcagggtc aggcccggcg cagcacgccg tgcttcttct tgcccaggct cagcttgccc
     2761 tcatgcacca ccagcgccgg atcggtgatc accgcatcat ccagtttcac ggcgccttcg
     2821 gccaccttgc gcttggcctc gccgtttgat gcggtgaagc cgatggcggt gagcgcggcg
     2881 atgatggcaa ggccttctgc cggcacatcc agcaccggca gatcagcgcc caggccgccg
     2941 ccggcaaagg tggcggcagc ggtggcctcg gcggcgcgag cggcttcctc gccgcggcac
     3001 agcttggtca cctcattggc cagcaccacc ttggcggcgt tgatctcgct gccttccagc
     3061 gcttccagcc gggcgatctc gtccagcggc agatcggtga acagcttgag gaagcggccc
     3121 acgtcgcggt cgtcggtgtt gcgccagtac tgccagaaat cataggccgg cagcgcgtcc
     3181 tcattcagcc acacagcgcc gttcatcgtc ttgcccatct tcgcgccatc ggcggtggtg
     3241 agcagcggcg tcgtcacgcc ataaacctcg gtgccatcca tccggcgggc cagttcgatg
     3301 ccgttgacga tattgcccca ctggtccgat ccgcccatct gcaggcggca gccatagcgc
     3361 tgcgccagct cgcggaaatc atagccctgg aggatcatgt agttgaattc gaggaaggac
     3421 agcgactgct cgcgatcgag ccgcagcttc accgaatcga acgacagcat gcggttgacc
     3481 gagaaatgct ggcccacctc gcgcaggaag gcgatatagc ccagcttgtc cagccactcg
     3541 gcattgtcga ccatgatggc atcggcaggg ccatcgccaa aggtcaggaa gcggtcgaag
     3601 atcttgcgga tcgaggcgac attgccggcg atgatctcgt cggtcatcag cttgcgcgcc
     3661 tcgtccttga aggagggatc gccgatcttg ccggtgccgc cgcccatcac cacgatgggc
     3721 ttgtggccgg cctgctgcag gcggcgcagc agcatgatct gcaccaggct gcccacatgc
     3781 agcgatggcg cggtgggatc aaagccgata tagccgggca ccacctgcgt ttgcgccagc
     3841 ttgtcgaggc cgtgggcatc ggtcagctgg tggatgtagc cgcgctcgtc gagcaggcgg
     3901 agcagggtgg actggtatgc ggacatgggc tctctctttt cctcgaaggg cgcgcctagc
     3961 ctgatttggc gcggaagaaa agcacatggc cttggggcaa acggacaaag atacagcagt
     4021 tgaagccctc gcggcgccag cgggccattt cgccctggtg tgccatccgg cacggccggc
     4081 aagggccgtg cgcgccgtca gcgtggactg gcgggcggat ggcgatcatt tcgtgctggg
     4141 ctggcaggtg gcgggcgcgg cgcatctggt ggtgccgccg gcggtggcgc ccgcccgcgc
     4201 agatggcctg tggcagacga cctgctttga actgttcctt gccgatgccg gcacggccta
     4261 ctgcgaattc aacctctcgc cctccacgcg ctgggcggcc tatcgctttg ccgcctatcg
     4321 cgatggcatg gcgccgcagg agatgccgca gccgccgcag atcacgccgg gcgctgcggg
     4381 cggcgctgtg tttgcctgca cggtgcggct gccggcggcc ttgctggcgg gggcccgggc
     4441 cgcgggcctg tgcgcggtga tcgaggaggc cggcgggcac ctgtcctact gggcgctggc
     4501 gcatggcccc ggccagcccg atttccacgc aagcgcttgc tttgcgctgg cgattggggc
     4561 agccgaggcg ccatgacgca agtgctcttc ggtatcgacc gcctgctggc agatccttcc
     4621 ctgcgcgcgc cgctggccgg caggcgcgtg gcgctggtgg cgcatccggc ctcggtcacg
     4681 gccggcctcg tccacagcct cgatgcgctg gccgggcttg gcgatatccg cctggcttcc
     4741 gcctttggcc cgcagcacgg gctgaagggc gacaagcagg acaatatggt ggaaaccggg
     4801 gacgagatcg atgccgccct cggcatcccc gtcttcagcc tctatggcga ggtgcgccgg
     4861 cccaccgaag cgatgatggc cagcgccgat gtgttcctgt tcgacctcca ggatctgggc
     4921 tgccgcatct acactttcgt gacgacgctg ctctatctgc tgcaggcggc cagcggcacc
     4981 ggcaaggccg tgtgggtcct ggaccggccc aaccccgccg gccgcccggt ggagggcacg
     5041 ctgctgctgc cgggcgagga aagcttcgtg ggggcagggc ccatgcccat gcgccacggc
     5101 ctcacgctgg gcgagatggg gcactggttc atccgccatt tcggactgga tgtggattat
     5161 cgggtgatca ccatggaagg ctggcagccc catgccgcgc ccggccatgg ctggccggaa
     5221 agccgcatct gggtgaaccc cagccccaat gccgccagcc tcaacatggc gcgcgcctat
     5281 gccggcacgg tgatgctgga aggggcgacg ctgtcggaag ggcggggcac cacgcggccg
     5341 ctcgaagtgc tgtttggcgc gcccgatgtg gcggcagggg cagtgctggc cgaaatgcgg
     5401 gcgctggcgc cgcaatggct ggccggctgc gccctgcgcg aatgctggtt cacccccacc
     5461 ttccacaagc atgccgggca attgtgcagt gcgctgatgg tgcatgccga aggggcgttc
     5521 tacgatcacg ccgcctttcg cccctggcgc ctgcaggcgc tggccttcaa ggcgatccgc
     5581 cggctttatc cggattatcc gctctggcgc gattttccct atgaatatga gtatgaccgc
     5641 ctggcgatcg atgtgatcaa cggcggcccg gccctgcgcc agtgggtgga tgatgccgct
     5701 gctgtgccgg ccgatctcga agcgctggcc agccatgacg aggcccgctg gcaggaggaa
     5761 cgggcgcctt tcctcctcta ttgagggccg cccgtattga ggctagcccg tgcccccgca
     5821 agcgccctag ccgcgcttgc gggacagttc gcgcatcgcc ccatccagcc cttccagcgt
     5881 cagcgcgaac atgcgcccgc cgatgatctc gcgcacctgc ctggtggatt gcatgtaggc
     5941 ccagcggctt tccggcaccg ggttcagcca cacgctggcc ggataggtgc gcgccaccct
     6001 ttccagccac acgccgccgg cctcctcgtt ccagtgctcc acgctgccgc ccggatggct
     6061 gatctcatag gggctcatcg ccgcatcgcc gacgaacacc accttgtaat catgcccata
     6121 ggtgtgaagg atatcccatg tggcggtgcg ctcggcaaag cggcggcggt tgtccttcca
     6181 cacgccctca tagaggcaat tgtggaagta gaagaattcc aggttcttga attcggtggt
     6241 ggcggcggaa aacagctcct ccaccaggcg gatataggga tccatcgagc cgccgacatc
     6301 gaggaacagc agcaccttca ccgcattgtg ccgctctggc cgcaggcgga tgtcgagcca
     6361 gccctgccgc gcggtgccct ggatggtggc atccaggtcc agctcctcgg ccgcgccctc
     6421 gcgggcaaag cggcgcaggc gtttcagcgc caccttgatg ttgcgcgtgc ccagctcgcg
     6481 cgtggcgtcg aaattggcga attcgcgctt ttcccacacc ttgatcgcgc ggttgtggcg
     6541 cgattggccg ccaatgcgga tgccttccgg attatagccg ccattgccga agggcgaggt
     6601 gccgccagtg ccgatccact tgttgccgcc ctggtggcgc ccctgctgct cggccaggcg
     6661 ctgcttcagc gtctccatga tctcctccca gctgccgagg gactggatct tctccatctc
     6721 ctcggggctg aggaactttt cggcgacggc gcgcagccat tcctcgggga tctcggcggc
     6781 cagttcgctc tcgcccagcg cgccgcgaaa cacccgggcg aacacctggt cgaaacggtc
     6841 gatcaggctc tcgtccttca catagatggc gcgggcgaga tagtagaagg cctcgggtgt
     6901 gggctcgatc gcctgcttct ccagcgcttc cagcagcagc agatgctctt tcagcccggc
     6961 cttgatgccg gccccgcgca gcgcttccag aaagccggtc agcatggcgc catcagatct
     7021 tgtggggggc ggccggcggc gccttgggat aggcgggcag atcgggatcg aactttaccc
     7081 aggagggcgc gctggcggtc cagatgatcg cctgcggggc gatgaggttg gggttgtcga
     7141 gcacgccggc gcgcaggcgg atgggctgac ccggattgtc gagcgggcgg ctgaagatct
     7201 gcgcgccgca ttccgggcag aagccgcgtt ccaccacatt gccgctgtcg gcgctgcggt
     7261 ggtaggtggc gatctcgccg gtgaaggaga aggcctcggc cgggaacagc acgttcaccg
     7321 tggccgagcc cgaggcaatg cgctggcaat cgcggcacca gcacatgcgc gcgcccaagg
     7381 gcgctgcatc cactgaaaac cgcacctgcc cgcacaggca gccgccttcg tgggggcctt
     7441 gttgcagatg gtctgacatg tttgctctcc ttgctgtcag ccgccgcgcc gggccatgaa
     7501 ggccagccgt tcgaacagca tgatatcggc ctcgttcttc agcagcgcgc catgcagcgg
     7561 cgggatcgcc ttggcgccat cgcggctggc cagcacgtcg agcggcatgt cctcggccag
     7621 cagcagcttc agccagtcga tcagctcgct ggtggaaggc ttcttcttga ggccgggcac
     7681 ttcgcgcaat tcgtagaaca tgtcgagcgc gcggtgcacc agcaccttct ggatgccggg
     7741 gaaatgcaca tcgatgatcg acagcatcgt ctcgcgctcg gggaacttga tgtagtggaa
     7801 gaagcagcgg cgcaggaagg cgtcgggcag ctccttctcg ttgttgctgg tgatgaccac
     7861 caccggccgc tcgcgcgcgg caatccgctc gccggtttca tagacatcga aggccatgcg
     7921 atccagctcg tggagcaggt cgttgggaaa ttcgatgtcc gccttgtcga tctcgtcgat
     7981 cagcagcacc ggcagccgtt cggatgcgaa ggcctcccac agcttgccgc ggcggatgta
     8041 gttggcgatg tcatgcaccc gcggatcgcc cagctggcca tcgcgcagcc gcgccaccgc
     8101 atcatattca tacaggccct gatgggcgcg ggtggtgctt ttcacatgcc attcgatcag
     8161 cggcgcatcc agcgcgcggg caatctcctg ggccagcacc gttttgccgg tgcccggctc
     8221 gcccttcacc agcaagggcc ggcgcagcag cacggcggca ttcaccgcca ctttcagatc
     8281 ctcggtggca acgtaattct cggtgctgtc aaaccgcggg gatgcttcgg ccattcgttt
     8341 gccctgcctt tcgcgccaga tggttaggaa gcgggccggg cgatggcaag cggcatttct
     8401 gagagggggc gacaggcggt ggcgggtcgc tgtcgggccg ttggcgggtg gctgacgggg
     8461 caccggcggc cattgtcggc cagcagacgt tgcggcaaaa gggcgtttgc ctaggcacgg
     8521 gccccacgag acgaggcgac acatgctgat ccaggaaatg cggttgaaac ggggatggtc
     8581 gcaacagcag ctggccgatc tgagcgggct gagcgtgcgc accatccagc gcatggagca
     8641 gggccagccg gccgcgctcg aatcgtacaa ggcgttgggc gcggcgttca acgtcgattt
     8701 tgccgagctt caggagcaaa ccgtgcgaga catgacaagc gatgccgaca agaccgagct
     8761 ggccctggcc tttgcccatg tgcgggcggt gcggaaattc tatttcggcc tgttcaccta
     8821 tgtggtggtg atggcgctgc tggcggggat caaccttgcc acctcgccgc atcacctgtg
     8881 gttcgtcttc ccgctgctgg gctggggcgt ggggctggcc ctgcgggcaa cgcgggtgtt
     8941 tggcctgctg ccgtggtttg gcccggaatg ggaaaaggcg caggtggaaa agcggctggg
     9001 ccggccgctg gcct
//
LOCUS       DASRYE010000026         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_195736,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000026 DASRYE010000000
VERSION     DASRYE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195736"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..980
                     /gene="lipA"
                     /locus_tag="VFF98_01475"
     CDS             21..980
                     /gene="lipA"
                     /locus_tag="VFF98_01475"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZU62326.1"
                     /translation="MTQAISTAAPAPSADEAQPATQRKPDWIRVKAPVSKEYGKTREL
                     MRGLSLNTVCEEAACPNIGECWTKKHATVMILGDVCTRACAFCNVKTGMPRKVDASEP
                     AHVAEAAAKLGLEHIVITSVDRDDLPDGGAGQFVKVINELRRNTPNTTIEILTPDFRG
                     KMRAAIEAIVAAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKAHNPLIFTKS
                     GVMLGLGEERLEVHQVMDDMRSAQIDFLTMGQYLRPTPKHAEVKEFVTPQAFGAYGAI
                     ARAKGFLQVAASPLTRSSYHAGEDFAQMRAAREAQLARQAAKG"
     gene            988..1419
                     /locus_tag="VFF98_01480"
     CDS             988..1419
                     /locus_tag="VFF98_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HZU62327.1"
                     /translation="MPGIHETRHLPYSPEQMFDLVADVARYGEFLPWVVATRVKSDDG
                     QEMVADMLVGFKALREKFTSRVTKDRPGQISVHYIDGPMRDLDNCWQFRPAADGGCEV
                     EFNVSFTFRNALFERIAGQYFDKAFRKMVEAFEARAAALYG"
     gene            complement(1447..1944)
                     /locus_tag="VFF98_01485"
     CDS             complement(1447..1944)
                     /locus_tag="VFF98_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZU62328.1"
                     /translation="MTDTLIPAEIYELAARVVSENAAQGRKLVLAESCTGGLVASALT
                     EVAGSSAVLEASFVTYSNAMKSRMLGVPADIIDTFGAVSVACAWAMAQGALKHSDADV
                     AVAISGIAGPDGGSAQKPVGLVVFAKAVRGSDEVLAEERQFAPTSRAAVRAEAVKVAL
                     ELLLP"
     gene            complement(1941..2480)
                     /locus_tag="VFF98_01490"
     CDS             complement(1941..2480)
                     /locus_tag="VFF98_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62329.1"
                     /translation="MFLSRSLLLAPLSALLAVIPGVARAAEPPCLTVREFTAVATYAL
                     PDILHGVGAACVPVLGQGAFLAQEGPALADRYAAARPAAWPATRAALARISASQGLAG
                     ALVARLPDATQQQLFSTLVAGVVAEKVPAQRCGLIDQILSLIAPLPPENTAAIIGMAV
                     GLGARSGQARMGKFQVCTE"
     gene            complement(2515..3678)
                     /locus_tag="VFF98_01495"
     CDS             complement(2515..3678)
                     /locus_tag="VFF98_01495"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HZU62330.1"
                     /translation="MTATANLDGRRVAAVVVAAGQGLRAGQAVPKQFAPWRGKPVLRH
                     SVEALARAGVAPMLVCIPEGADAIATAALAGLAGVRFVTGAATRQGSVKAGLEALAAD
                     APDLVLIHDAARPILPQAVITRLVAALGQQPGAIPVLPVVDSLCHAEGGLMGAPAVRE
                     ALRRVQTPQAFRYKDILAAHRAWGDAPNAGDDAQVARAHGLAVALVDGDEALHKLTFA
                     SDFGGDAPALPPVRMGTGYDVHRLEEGEELWLCGVKIDHSHGLSGHSDADVAIHALVD
                     AILGAGALGDIGQHFPPSDPQWKGASSDRFLAHAVQLAGAAGYAIGHVDVTIICEAPK
                     IGPHRNAMRERLAGIMGVDCALVSVKATTTEKLGFTGRGEGIAAQAAATLIRR"
     gene            3755..>3912
                     /locus_tag="VFF98_01500"
     CDS             3755..>3912
                     /locus_tag="VFF98_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZU62331.1"
                     /translation="MTETSPTPQPPVPPAIAPISVGGVMIDCPVVLAPMTGVTDLPFR
                     RMVRRYGSG"
BASE COUNT          659 a         1349 c         1329 g          575 t
ORIGIN      
        1 cggcctatct gccccctgtc atgacacaag ccatcagcac cgcagcgccc gccccttcgg
       61 ccgatgaggc ccagcccgcc acccagcgca agcccgactg gatccgggtg aaggcaccgg
      121 tgagcaagga atatggcaag acgcgcgagc tgatgcgcgg cctctcgctg aacaccgtgt
      181 gcgaggaggc ggcctgcccc aatatcggcg aatgctggac gaaaaagcac gcgacggtga
      241 tgatcctggg cgatgtgtgc acgcgcgcct gcgccttctg caacgtgaag accggcatgc
      301 cccgcaaggt ggacgccagc gagcccgccc atgtggccga agccgctgcc aagctggggc
      361 tggaacatat cgtcatcacc tcggttgacc gtgacgacct gcccgatggc ggcgccggcc
      421 agttcgtgaa ggtgatcaac gaattgcgcc gcaacacgcc gaacaccacg atcgagatcc
      481 tgacgcccga ttttcgcggc aagatgcgcg cggcgatcga ggcgatcgtg gcggcgggtc
      541 cggatgtgta taaccacaat ctggaaaccg tgccgcggct ttatcccact atccgccccg
      601 gcgcgcgcta ttatgcctcg ctgcggctgc tggaagaggt gaaggcccac aatccgctga
      661 tcttcaccaa gagcggcgtg atgctggggc tgggcgagga acgactcgag gtgcatcagg
      721 tgatggacga catgcgcagc gcgcagatcg atttcctgac gatgggccag tatctgcgcc
      781 ccacgcccaa gcacgccgag gtgaaggaat tcgtcacgcc ccaggccttt ggcgcctatg
      841 gcgcgatcgc ccgggccaag ggcttcttgc aagtggcggc cagcccgctc acccgctcgt
      901 cctatcacgc cggcgaggat ttcgcccaga tgcgcgccgc gcgggaagcc cagctggccc
      961 gccaggccgc caagggctga ggcacccatg cccggcatcc acgaaacccg ccatctgccc
     1021 tatagccccg agcagatgtt cgacttggtc gccgatgttg cgcgctatgg cgaatttctg
     1081 ccctgggtgg tggccacccg cgtcaagtct gatgacgggc aggagatggt ggccgacatg
     1141 ctggtgggct tcaaggcgct gcgcgagaag ttcaccagcc gggtgacgaa ggatcgcccc
     1201 ggccagatca gcgtgcatta tatcgacggg ccgatgcgcg atctcgacaa ttgctggcag
     1261 ttccgcccgg cggccgatgg cggctgcgag gtggagttca acgtcagctt caccttccgc
     1321 aatgccctgt tcgagcgcat tgccggccag tatttcgaca aggccttccg caagatggtg
     1381 gaagccttcg aggcgcgggc ggcagcgctt tatggctgag ctgcggccgc ccgcggcctg
     1441 aaacggtcag ggcagcagca gctccagcgc caccttcacg gcctcggcgc gcacggcggc
     1501 gcggctggtg ggggcaaact ggcgctcctc ggccagcact tcgtcgctgc cgcgcacggc
     1561 cttggcaaac accaccagcc ccaccggctt ttgcgcgctg ccgccatcgg gcccggcaat
     1621 gccgctgatc gccacggcca catcggcatc ggaatgcttc agcgcgcctt gcgccatggc
     1681 ccaggcgcac gccaccgaca ccgcgccgaa cgtgtcgatg atatcggccg gcacgcccag
     1741 catgcggctc ttcatcgcgt tggaataggt gacgaagctg gcctccagca cggcggagga
     1801 gccggccacc tcggtcagcg cgctggcgac gaggccgccg gtgcagcttt cggccagcac
     1861 cagcttgcgc ccttgcgcgg cgttctcgct caccacgcgg gcggccagct cgtagatctc
     1921 tgcggggatg agggtgtcag tcattctgtg caaacctgga atttgcccat gcgggcctgg
     1981 ccgctgcgcg cgccaaggcc caccgccatg ccgatgatgg cggccgtgtt ttccggcggc
     2041 agcggggcga tgagggaaag gatctggtcg ataaggccgc agcgctgcgc cggcaccttt
     2101 tcggcaacaa cgccggccac cagcgtggag aacagctgct gctgggtggc atcgggcagg
     2161 cgggccacca gcgcgccggc gaggccctgg ctggcgctga tgcgggcaag ggcggccctg
     2221 gtggcgggcc atgcggccgg ccgcgcggcg gcatagcgat cagccagcgc cgggccttcc
     2281 tgcgccagga aggcgccctg gcccagcacc ggcacgcagg ccgcccccac gccatggagg
     2341 atatccggca gggcataggt ggccaccgcg gtgaattcgc gcaccgtgag gcaagggggc
     2401 tcggccgcgc gggccacccc cgggatcacg gccaacagcg ccgacagcgg cgccagcagg
     2461 agggatctgg acaggaacat ggggggcaat ctcctgtggg cggggcgggt gcgctcagcg
     2521 gcggatcagc gtggcggcgg cctgcgcggc aatgccttcg ccgcgcccgg tgaagcccag
     2581 cttctcggtt gtcgtggctt ttacactgac cagcgcgcaa tcgacaccca tgatcccggc
     2641 caggcgctca cgcatagcat tgcggtgggg gccgatcttg ggcgcctcgc agatgatcgt
     2701 cacgtcgaca tggccgatgg catagcccgc cgcgccggcc agttgcaccg catgggcaag
     2761 gaagcgatcg gaagaagcgc ccttccactg cggatcggaa ggggggaaat gctggccgat
     2821 atcgccaagc gcgccggcgc ccagaatggc atcaaccagc gcatggatcg ccacatcggc
     2881 atcggaatgg ccggaaaggc cgtggctatg gtcgatcttc acgccgcaca gccacagttc
     2941 ctcgccttcc tccaggcggt ggacatcata gcccgtgccc atgcgcacag gcggcagggc
     3001 aggggcatcg ccgccaaaat ccgaggcaaa ggtcagcttg tgcaaggctt cgtctccatc
     3061 caccagggcc accgccaggc catgcgcgcg ggccacctgg gcatcatcgc ccgcattggg
     3121 ggcatcgccc caggcgcggt gcgcggccag aatatcctta tagcggaaag cctgcggggt
     3181 ttgcacccgg cgcagcgcct cacgcacggc aggcgcgccc atcagcccgc cctcggcatg
     3241 gcacaggctg tccaccaccg gcagcaccgg aatggcgccg ggctgctggc ccagcgctgc
     3301 caccagccgg gtgatcaccg cttgcggcag gatcgggcgg gcggcatcgt ggatcagcac
     3361 cagatcgggg gcatcggccg ccagcgcttc cagcccggcc ttcaccgagc cctgcctggt
     3421 ggccgccccg gtgacgaagc gcacgccggc aaggccggcc agcgccgccg tggcaatggc
     3481 atcggcgcct tcgggaatgc acacgagcat gggggccacg ccggccctgg ccagcgcttc
     3541 caccgaatgg cgcagcaccg gcttgccgcg ccagggggca aactgtttgg gaacggcctg
     3601 gccggcgcgc aagccctggc cggcggcgac gaccacggcg gccacgcgcc gcccgtcgag
     3661 attcgctgtc gctgtcatca ggcgggcgcc taatcgcttg ccgcagcccg cgcaatccac
     3721 tatgcgctgc ctgtttttta ggcagcagca gaacatgacc gaaaccagcc ccacgcccca
     3781 gccgcccgtt ccgcccgcca tcgcgccgat cagcgtgggc ggggtgatga tcgattgccc
     3841 ggtggtgctg gcgccgatga cgggtgtgac cgatctgccc ttccgccgga tggtgcgccg
     3901 ctatggctcg gg
//
LOCUS       DASRYE010000027         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_107527,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000027 DASRYE010000000
VERSION     DASRYE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107527"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFF98_01505"
     CDS             complement(<1..992)
                     /locus_tag="VFF98_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU62332.1"
                     /translation="MRKTGEFRRTAGAATMAFALATASAAHAADTDNVPVVTKAGPAT
                     DQAEIPILVMAGNNKSAILPKSSDAFGLGLSQVETPRSSSNVSLAMIDQYSLTTIRDL
                     IAVTPGVFTASFYGVDGSVNIRGEYADNYFLGFKRVENQGTYITPIESALDLEVVRGI
                     ASPAYGTGRAGGYVNFDPRSNRAERFMNGQKAFGELDGAIGTYSMYRGEVEYGAPLHW
                     GDYNAGFDIYYEHLQADQYFYGIHPKSNLLQAGFATELPADWTFKANYQYYRGSGMQG
                     TTGINRITQALVDNGTYTAGHPIAQIVQAGQAYITPADINAIGGVTNYYATSNN"
     gene            complement(1067..2110)
                     /locus_tag="VFF98_01510"
     CDS             complement(1067..2110)
                     /locus_tag="VFF98_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HZU62333.1"
                     /translation="MSTTELRYDSADTLLADEIPVIDLAPFLHGDAAARAGVAAAIAA
                     ACERIGFFYVINHGVPEGLRQAVLEEARGFFARPLAEKMACAPIADGRWCGYLPGRGA
                     AEGTRPGGSLTAEGQATTSVSGGTLQQFNMMRVLPADDANYGNPDLLYQRNPWPAGQP
                     GFVETMVVNQRALLALGAQLMRAFAIALGLEETHFLRLHQSPLATFGLNYYPRPVAGA
                     ASADVGVGAHCDASSFTVLLQDEIGGLEVQTRDGRWIAAPVVPGAYVINIGDTMMGWT
                     NGHFVSTLHRVVNRRGIDRFSATMFMNPDREVTIAPLAPFVSAENPARFQPFANEDYM
                     RRFFVKFYDSMFK"
     gene            complement(2107..3129)
                     /locus_tag="VFF98_01515"
     CDS             complement(2107..3129)
                     /locus_tag="VFF98_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU62334.1"
                     /translation="MNERVSRLLDFLFSREGVMVVVVAALFAGFAASAPHFLESQTQF
                     EILRLTAFTLIIGLPLTALVIAGEVDLSIGSTYGLASVVCAMAIVQWGWNPWAAGLLA
                     AMLGLAVGLVNGWAVVRLKVPSFIQTIGMLSLLRGLALVLTMGLSIDIPASIESRFFG
                     FAGGMLGPVPVQVLWALLAFVLGGVVLRHTVFGAHLYATGGNARAALEMGIRPGRVKL
                     WTFAVLGLSCGLAGALTTGWLRIGAPSTGNGGELSIIAAVIIGGAALSGGVGTALGTL
                     LGAFVVSMLQTGLILSGVQGNWVQVGMGALIVLAAGIEPHFRPDGPLADRLRAFGRTQ
                     AEAGDA"
     gene            complement(3126..3926)
                     /locus_tag="VFF98_01520"
     CDS             complement(3126..3926)
                     /locus_tag="VFF98_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62335.1"
                     /translation="MGWPERLAPSPVAASQRAAPEATGPAVLGARGLSRHFGRVAALE
                     GVDLDLAAGSVTALLGDNGAGKSTLIALLSGVLAPSAGEILIDGAPCRFADPAAARSA
                     GVATVFQKLSLVDDRSIAENLFLGAEPLRFGCFIDRARMQREARSVLGRLHVNLPPVT
                     TAVRALSGGQRQCVAVARTLLRGSRVVILDEPTAALGLRETRRVLDLIARLRDEGRAV
                     LLVSHNMDNVFDVADRALVLRLGRKVADVALAAVSRADLVGLIVGGAA"
     gene            complement(3913..4911)
                     /locus_tag="VFF98_01525"
     CDS             complement(3913..4911)
                     /locus_tag="VFF98_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU62336.1"
                     /translation="MLGAAMALAACSHGDAGKGAAALGPARTVAFAVGTPGSDFVTDI
                     SLGCADAAAMLGWTFRRVLNPQPTPDAHITAIRQAVTARSDVIVTVDWYQAVVDEIAA
                     GQKQGAHFALVNSANNPASLAGLGVPFAGQQPRDNGRVMGQRMAEALKARGLAAGSVL
                     VGNPFPGSANVEERIAGIGEGLASMPGLRMVSFPDGSAQDPVASVGLYRAKVVELGNV
                     VGHAAAGGEMSAVPLLKALSGMAPGAGGRPVVAGWSSSLAQLNMVKAGRIDFALDENL
                     YYQGFLAVFMAWSMIERGLPATSLAAGHSWVEPATVTAMIASYETRRQRAVAYGLA"
     gene            complement(5072..5983)
                     /locus_tag="VFF98_01530"
     CDS             complement(5072..5983)
                     /locus_tag="VFF98_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62337.1"
                     /translation="MLSRFAVYFDEAARHRSIRRASEHLRIAPSAIDRQILKMEEQLG
                     VPLFVRLPQGLQLTAAGEMMVASIRRWRRELRSLEAQIDELRGLRRGEVTLALAEGSG
                     DLAIRGLALFRQRYPGIHCRMTVAASQEIVDLVLRGEVEIGLTFNPPDRRELRVERAM
                     VYQIGAVMLPDHPLADQASTTLADCAAHPLVGPEAGHSLRVVLDRAWELQLGTSPRFV
                     ATANSVSLIRSMVLGGIGLGLLTPIDVASELEQGLLRYVPLEGSNIPLSVLSLVTASG
                     RPLSPPGSLLIRHFTAEAVEDARILDC"
BASE COUNT         1116 a         2208 c         1966 g          930 t
ORIGIN      
        1 taattattcg aggtggcgta atagttggtc accccgccga tggcgttgat atcggctggg
       61 gtgatatagg cctggcccgc ctgcacgatc tgcgcaatcg ggtggccggc ggtgtaggtg
      121 ccattgtcga ccagcgcctg ggtgatgcgg ttgatgccgg tggtgccctg catgccgctg
      181 ccgcgataat actggtaatt ggccttgaag gtccagtcgg cgggcagttc ggtggcaaag
      241 ccggcctgca gcaggttgct cttggggtgg atcccataga aatactggtc ggcttgcagg
      301 tgctcgtaat agatgtcgaa gccggcgtta taatcgcccc agtgcagcgg cgcgccatat
      361 tccacctcgc cgcgatacat gctgtaggtg ccaatggcgc cgtccagctc gccaaaggcc
      421 ttctggccgt tcatgaagcg ctcggcgcgg ttgctgcgcg gatcgaaatt gacatagccg
      481 ccggcccgcc cggtgccata ggccggcgag gcgatgccgc gcaccacttc cagatccagc
      541 gcggattcga tcggcgtgat ataggtgccc tggttttcca cccgcttgaa gccgaggaaa
      601 taattgtcgg catattcgcc gcggatgttc accgatccat ccacgccata aaagctggcg
      661 gtgaacacgc cgggcgtgac ggcgataaga tcgcggatgg tggtgaggct gtattgatcg
      721 atcatcgcca gcgacacatt gctggaagag cgcggcgttt ccacctggct gaggcccagg
      781 ccaaaggcat cgctgctttt gggcaggatg gccgatttgt tgttgcccgc catcaccaga
      841 atcgggatct cggcctggtc tgtcgccggg ccggccttgg tgacaacagg cacgttgtcg
      901 gtgtctgcgg catgggcggc actggccgtg gccagggcaa acgccatcgt tgccgcaccc
      961 gcggtgcggc ggaactcccc tgtttttctc atcttaccct cacattctcg aaaaggctga
     1021 cctggccttt ttcgggccct cccccgggct cagttgcgct gtggcgtcat ttgaacatgc
     1081 tgtcgtagaa tttgacgaag aagcggcgca tatagtcctc gttggcaaag ggctggaaac
     1141 gcgccggatt ttcggccgaa acgaaaggcg ccagcggggc gatcgtcacc tcgcggtccg
     1201 ggttcataaa catggtggcg gaaaagcggt cgatcccccg gcggttgacc acccggtgca
     1261 gggtcgagac gaagtggccg ttggtccagc ccatcatcgt atcgccaatg ttgatgacat
     1321 aggcccccgg caccaccggc gctgcaatcc agcgcccgtc gcgcgtctgc acttccagcc
     1381 cgccgatctc gtcctgcagc aacacggtga agctggaggc atcgcaatgg gcccccacgc
     1441 ccacatcggc agaggctgcc ccggccaccg gccgcgggta ataattcagc ccgaaggtgg
     1501 cgagcgggct ttggtgcagg cgcaggaaat gcgtctcctc cagccccagg gcgatggcaa
     1561 aggcgcgcat cagctgcgcg cccagcgcca gcagcgcccg ctggttcacc accatcgtct
     1621 cgacaaagcc gggctggccg gccggccacg gattgcgctg atagagcaga tcgggattgc
     1681 cataattggc atcgtctgcc ggcagcacgc gcatcatgtt gaactgctgc aaggtgccgc
     1741 cagaaacgct ggtggtggcc tgcccctcgg cggtgagcga gccgcccggc cgcgtgcctt
     1801 cagccgcccc gcgccccggc agatagccgc accagcgccc atcggcgatg ggggcacagg
     1861 ccatcttctc ggccagcggg cgggcaaaga agccgcgcgc ttcctccagc accgcctggc
     1921 gcaagccttc gggcacgcca tggttgatca catagaagaa gccgatccgc tcgcaggcag
     1981 cggcaatggc ggcggccacg cccgcgcgcg ctgccgcatc gccatgaagg aagggggcca
     2041 gatcgatcac cgggatctcg tctgccagca gcgtatcggc gctgtcgtag cgcagttcgg
     2101 tcgtgctcat gcgtcgcctg cctctgcctg ggtgcgcccg aaagcgcgta atctgtcggc
     2161 cagcgggccg tccgggcgga aatgcggctc gatgccggcg gccagcacga tcagcgcgcc
     2221 catgcccacc tgcacccaat tgccctgcac gcccgaaagg atcagcccgg tctgcagcat
     2281 ggagacgaca aaggccccca gcagcgtgcc cagcgccgtg cccacgcccc cgctcagcgc
     2341 cgccccgccg atgatgaccg cggcaatgat cgacaattcg ccgccattgc cggtggaagg
     2401 cgctccaatg cgcagccagc ctgtcgtcaa cgcgccggcc agcccgcaag acaggcccag
     2461 cacggcaaag gtccacagct tcacccggcc cgggcggatg cccatttcca gcgccgccct
     2521 tgcattgccg ccggtggcat agagatgagc gccaaacacc gtgtggcgca gcaccacgcc
     2581 ccccagcaca aaggccagca gcgcccacag cacctgcacc ggcaccggcc ccagcatgcc
     2641 cccggcaaag ccgaagaagc ggctctctat cgaggcggga atgtcgatcg acaggcccat
     2701 cgtcagcacc agcgccagcc cgcgcagcag gctgagcatg ccgatcgtct ggatgaagga
     2761 gggcaccttg aggcgcacca ccgcccagcc gttcaccagc cccaccgcca gccccagcat
     2821 cgccgccagc aggcccgccg cccaggggtt ccacccccat tgcacgatcg ccatggcgca
     2881 caccacgctg gccagcccat aggtggagcc gatcgacaga tcgacctcgc cggcgatcac
     2941 cagcgctgtc agcggcaggc cgatgatcag cgtgaaagcg gtaagccgca ggatctcgaa
     3001 ctgggtctgg ctttcgagga agtggggggc gctggcggca aagccggcaa acagcgcggc
     3061 caccaccacc accatcaccc cttcgcggct gaacaggaaa tccagcaggc gggagacgcg
     3121 ctcgttcatg ccgcccctcc cacgatcagc cccaccagat ccgcccggct gaccgccgcc
     3181 agcgccacat cggccacctt gcgccccagc cgcagcacca gcgcgcggtc ggccacgtcg
     3241 aacacattgt ccatattgtg gctcaccagc agcaccgcgc ggccctcgtc acgcaggcgg
     3301 gcgatgagat cgagcacgcg gcgcgtctcg cgcaggccca gcgccgcggt cggctcgtcc
     3361 aggatcacca cccgcgatcc gcgcagcagg gtgcgcgcca ccgccacgca ttgccgctgc
     3421 ccgcccgaca gcgcccgcac cgccgttgtc accggcggca ggttcacgtg cagccgcccc
     3481 agcaccgagc gcgcctcgcg ctgcatgcgg gcgcgatcga taaagcagcc aaagcgcagc
     3541 ggctctgccc ccaggaacag gttttcagcg atggaacgat catccaccag cgacagcttc
     3601 tggaacaccg tggcgacgcc tgcactgcgc gcggcggccg gatcggcaaa gcggcagggc
     3661 gcgccatcga tcaggatctc gccggcggaa ggggccagca cgcccgaaag cagcgcgatc
     3721 agcgtggatt tgcccgcccc attatcgccc agcagcgctg tcacactgcc tgccgccaga
     3781 tcgagatcga ccccctccag cgccgccacc cggccaaaat ggcgcgacag gccgcgcgcg
     3841 cccagcaccg ccggcccggt ggcctcaggc gcggccctct gcgaggcggc caccggcgag
     3901 ggcgccaggc gctcaggcca gcccatatgc caccgcccgc tgcctgcgcg tttcatagct
     3961 ggcgatcatc gccgtcaccg tcgccggctc cacccagctg tggccggccg ccagcgatgt
     4021 ggccggcagc ccccgctcga tcatcgacca ggccatgaac acggccagaa aaccctggta
     4081 atagaggttc tcgtccagcg cgaaatcgat ccgcccggcc ttcaccatgt tgagctgggc
     4141 gaggctgctg ctccacccgg ccaccaccgg ccggccgcca gcgcccggtg ccatgcccga
     4201 cagcgccttc aatagcggca ccgccgacat ctcgccgccg gccgcggcat ggcccaccac
     4261 attgcccagt tccaccacct tggcccgata gagccccacc gaggccaccg gatcctgcgc
     4321 cgaaccatcg ggaaagctga ccatgcgcag gcccggcatg ctggccagcc cttcgccaat
     4381 gccggcgatc cgctcttcca cattggcaga gccggggaag gggttgccca ccagcacgct
     4441 gccggccgcc agcccccttg ccttcaacgc ctcggccatc cgctggccca tcacccggcc
     4501 attgtcgcgc ggctgctggc cggcgaaagg cacgcccagc ccggccaggc tggcaggatt
     4561 gttggcggaa ttgacgaggg cgaaatgcgc cccctgcttt tgcccggcgg caatctcgtc
     4621 caccaccgcc tgataccagt ctaccgtgac gatgacatcg ctgcgcgccg tgaccgcctg
     4681 gcggatggcg gtgatatggg catcaggcgt gggctggggg ttcagcacgc ggcggaaggt
     4741 ccagcccagc atggcggcgg catcggcaca gcccagcgag atatcggtga cgaaatcgga
     4801 acccggcgtg cccacggcaa aggccaccgt gcgcgccggg cccagcgccg ctgcgccctt
     4861 gccggcatcg ccatggctgc aggcggccag cgccatggcg gcgcccagca tgggtgcgcc
     4921 tgcgaaaagc gcccgcctcg tcatttcatc tgccgcgccc tcggccatgc ccgcttcacc
     4981 ccgatgtcat tgatccagat caatgttgag gattggccgc ggataatcca gcgctaaaaa
     5041 aggcatctcg cattcgcaaa acggcaacag gctaacaatc gagaatgcgg gcatcctcca
     5101 ccgcctcggc ggtgaaatgg cggatcagca gcgatccggg cggcgacagc ggccgccccg
     5161 aggcggtgac cagcgacagc accgagagcg gaatgttgct gccctccagc ggcacatagc
     5221 gcagcagccc ctgctccagc tcgctcgcca catcgatcgg cgtcagcaag ccgaggccga
     5281 tgccgcccag caccatcgag cgaatcaggc tgacgctgtt ggccgtggcc acgaagcgcg
     5341 ggctggtgcc cagttgcaat tcccacgccc gatccagcac cacacgcagc gaatgccccg
     5401 cctccgggcc caccagcgga tgggcggcgc aatcggccag cgtggtggag gcctggtccg
     5461 ccagcggatg atccggcagc atcaccgcgc cgatctgata gaccatggcc cgctccaccc
     5521 gcaattcgcg ccgatcgggc ggattgaagg tgaggccgat ttccacctcg ccgcgcagca
     5581 ccagatcgac gatctcctgc gaggcggcca ccgtcatccg gcagtggatg ccaggatagc
     5641 gctggcggaa cagggcaagg ccgcgaatgg cgagatcgcc gctgccctcg gccagcgcca
     5701 gcgtcacctc gccgcgccgc aggccgcgca attcgtcgat ctgcgcctca aggctgcgca
     5761 attcgcggcg ccagcggcgg atcgaggcca ccatcatctc gccggcggcg gttagttgca
     5821 ggccctgagg cagccgcacg aacagcggca cgcccagttg ctcttccatt ttcaggatct
     5881 gccgatcgat cgccgaaggc gcgatgcgca gatgttccga ggcccgccgg atggagcgat
     5941 ggcgcgcggc ttcgtcaaaa tatacggcaa agcgggaaag catgggcaac atatctcgcg
     6001 cgcgcagccc ggcctggccg ggcgcggatg ggggaggcca aggtggcccc atcgcgctgg
     6061 ccccgtcaac acgtatcccc aaacgcaagc accccccctc gtcgtaaaac gagggggggt
     6121 gcttgcgtgt atgtggtgct tgtgatgggt ttttatacgc gggtagcaat gcctggcgac
     6181 gccctactct tccaacgctt gagcgttagt accattggcg
//
LOCUS       DASRYE010000028        13873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_177567,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000028 DASRYE010000000
VERSION     DASRYE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177567"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFF98_01535"
     CDS             <1..924
                     /locus_tag="VFF98_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62338.1"
                     /translation="AGLAALVFAGLVRWLWPTAPALLASAIVGGMGIAVVQALMPALI
                     RHRAGAQAPALMGFYSTAIMGGAALASFATPRLALAQGWAVAAGIWALPALIGLVIWW
                     LATDNSDEAAPAPPQSPVSRSRRSWLLLSFFGLGTGGYTLVLAWLPPYYTGLGWSAQD
                     AGTLLSALTLAEVAAGIGIAMAVGRFADRRALILLAIGALVAGLLCLCLAPLALAWCA
                     ALLCGLGIGALFPLGLIVAMDHGRDAAQAGQIAGFVQGGGYVLAALLPLIAGVLRQQL
                     SDLAPAWWLMAALCLVLALLAIRLRPGAYLT"
     gene            946..1821
                     /locus_tag="VFF98_01540"
     CDS             946..1821
                     /locus_tag="VFF98_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62339.1"
                     /translation="MAFYTVQERSNYYVSIGEGRPVLLLHGISNSGRAWAPQVPALVA
                     AGYRVIVPDHAGHGASAALSAPFGVQDIADDTEALIGQLGIDRFDLIGLSLGGMVALE
                     LALRHPSRIGRLIAANSFDRTATPAFAAMAAEWARLFRQPHGPVVRLEQNWPSLVSPT
                     FQKTADGLRTWQVWHGIAASADGPSLAHVARGITDFDRADRIGELAMPALFIAGGLDR
                     MSPPALSRAMAEQAPRGAYQLIEGAAHISNVDSADTFTQSMLRFLDPAFLDPAEPTAA
                     DQPLSGSSRGGMLGA"
     gene            1839..2567
                     /locus_tag="VFF98_01545"
     CDS             1839..2567
                     /locus_tag="VFF98_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU62340.1"
                     /translation="MASGLLAGSMNALAGGGSFVTLPALIASGVPSVMANATSSVALY
                     PGGVASAWVYRHGLGPVCRVPLPLMLAVTLSGGLAGALLLLWTPGAVFDRVLPLLLLL
                     ATLMLVFAPKAGGWLRDHVTIGRPMILPMQFVLGLYGGYFGGAVGLMMIAAWSLLQGA
                     DIKALNAPRTLMVTAANTVAIACFAIAGAVAWRSALLVGLGAVAGGYAGAHLGRRLSP
                     ALVRMATLALSALVTASFFIRAYA"
     gene            2647..3045
                     /locus_tag="VFF98_01550"
     CDS             2647..3045
                     /locus_tag="VFF98_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZU62341.1"
                     /translation="MTSRIIHTPDATALERLAHKAVKALPAVFRDHLGDVVFRVQDFA
                     ESAMLQAVGLSDPWALLGLYHGRPFGQHSIWSTGEMPSEIFLFRRPLLEACHQRGVPV
                     EEMVTHVIVHEVGHHFGLSDADMQAIEDEP"
     gene            3268..3429
                     /locus_tag="VFF98_01555"
     CDS             3268..3429
                     /locus_tag="VFF98_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62342.1"
                     /translation="MEWVPVVFITFKLIVFGTGMFFAIKWHYDQDKKKKNEASKQQAP
                     AEQTSDAAE"
     gene            complement(3530..4102)
                     /locus_tag="VFF98_01560"
     CDS             complement(3530..4102)
                     /locus_tag="VFF98_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62343.1"
                     /translation="MTCATFLFAILALLMAPGPTNTLLALAGAQQGWQRALRLIPAEM
                     LGYLATVPLLAWLGATSLARWPLAAPALKGAAAVWVLYLAVRLWRAPGRQGGPARVTR
                     RSVGLTTLLNPKALVVGLILIPAPGRPDFLPRLALFFLVVPLVALVWSGGGALAGRHG
                     SPARTRLIQRLASICLMVLSASLTAAVLRA"
     gene            complement(4197..5075)
                     /locus_tag="VFF98_01565"
     CDS             complement(4197..5075)
                     /locus_tag="VFF98_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU62344.1"
                     /translation="MRSLAVFEAAARHGKFAAAAGELAMTQAGVSQHIAQLESELGVV
                     LFHRRHRGVQLTDAGAKLLDAATDGLHTLARGVAEARRAGSGRTVNILTDFGFAAWWL
                     MPRIAELSELMPQVEIRLVTSQGGSDIGEPDFDLAVLFGGSDWPGCRARRLFAERVYP
                     VCAPAYAQAPLAPAQIADLRLLHLRHHGAQRWFEWDDWFAGMGITPPRRQQELVFSNY
                     QIVLQAVLLGQGVALGWAPLIDELIANGSLIRLCDTPLTSARGYHLIEPLRAAGGTNH
                     AARVADWLWQAERGAA"
     gene            5200..6717
                     /gene="betC"
                     /locus_tag="VFF98_01570"
     CDS             5200..6717
                     /gene="betC"
                     /locus_tag="VFF98_01570"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HZU62345.1"
                     /translation="MSAKPNFLVLMADQMAADVLPFMAQSPVIAPHMGKLAAQGVVFA
                     GACCPSPLCAPSRFSLLSGQLPSRIGAYDNACEFPAETPTIAHYLRAGGWHTILSGKM
                     HFVGPDQLHGFEERLTTDIYPADFSWTPDWTRPDHRPSWYHSMDSVLTAGPTIRTNQI
                     DYDEEVVFTARRKLYDLARARNDRPFFMVASLTHPHDPFAIQQRYWDLYEGVDIPMPR
                     VPFDRAALDPHSARLRHVCGADIDPVRPDQLRAARRAYFGAISFVDDQFGAILDTLAE
                     TGLAENTIVIVCGDHGEMLGERGLWYKMSFFEGAVRVPMVIHAPQRFAPRRVEASVST
                     LDLLPTLLDLAGLPAADAAPVDGRSLLPHLQGSAGPDEALGEYLGEGALAPIVMIRRE
                     GWKYIHSPTDPDQLYHLAADPDERTNLAADPAHAPRIAALRAEVQARWDLPEIAADVL
                     ASQRRRRLVAKANATGQLTHWDHQPPRDASRAYIRAHMDLEQLEAQARFPRVRFD"
     gene            6757..9060
                     /locus_tag="VFF98_01575"
     CDS             6757..9060
                     /locus_tag="VFF98_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62346.1"
                     /translation="MNHALVRALLLAGSVFPALPAHAEAAADTPADTAATGTRDIVVT
                     ATKRAANAQDVPLSISVLGGQAIARAQLRDGIAIARETPNLVAESVMGAAMPRYRLRG
                     IGTNDFAPTATSAVGVYEDEVYISAGSAQSEPLYDLDRVEVLRGPQGSLWGKNTTAGA
                     IHFVTAQPSDTTTGQIRAAYGTDNTQELEGGIGGKLADGLDARVAGVYKHRNGQFFNA
                     YSGQHVGGYDIWDLRGQLRYHIAPGATVTLKVHGGHSFQGQPLGHIGLLAGGADATGY
                     VQSSNPSVLSNAGSDYTGADRFGVDMRLDVDLGGARLIDIAAYEHSSSLIYSDDAANP
                     VNAYDERYGGHSGTFTNELRLQSADSGAFGWIAGLYYLRDKTESFGQLGLYGPVNFGV
                     NGDAYDFHVTTDNVAGFGSLNYQLTPRLKLTAGARFTWERKAIAGKAYEYVTQAGSVY
                     DASVPGLVYVNTATNTFVDTTGAAIAAVPLSRSWSKATWDASADYRISPGAHAFVRVA
                     RGFRSGAYNAYVANPGDLSVYNPETLTSYEGGIKTSWWARKLTLNVTAFHYNISNMQV
                     TVLQSVGSTTQNAAAARVNGFEVEARAHPLAGLSLGLGYGFQASKYTGFANASVPYPI
                     NQGAPLDLSGQSLERAPKHTLNLSGNYEHPLAGGTLFAGTDWRYTSRYRFQVWSDATN
                     TNPAPFLASAATQQLLHDAFSQNDLWLGDARIGWRAANGLEVAVWVKNLTDQVYKTNT
                     FGMFFNRSVSYYPGERRTAGVSASYRF"
     gene            9070..10248
                     /locus_tag="VFF98_01580"
     CDS             9070..10248
                     /locus_tag="VFF98_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62347.1"
                     /translation="MNRRQPWLALAAIGAATAAAHIGNNFTTYLVGGLIDRYHFSPSQ
                     MGVFAMSETLAYAAAMFGVAPRAASCSPRRLALAASTLVVAAQAGSAATSGFALLLAG
                     RVVAGLGFGLMNSAVNLAAGHQDHPARAISTGIMLQTLLYALVNIGLPMMGAAHGVGG
                     MFAALAGLSAVLGLATLRLPDTAAPAAHGQARAAPLPRQGWWVLAAMALFCAGSLAIW
                     PFMERAAHAIGLSAARFGRYQSLATLLSAGGNALLALLAGRIVRRWALTAALLACGLA
                     CAGLTTLTLPGAFAAALVVYNVSWFLAYPLLLGLAYAADPGGRLAVMTTATWLLAESA
                     AAMGAGTIAQTAGSYTIIGPLGLAGCVGALLLAWPLASRADAGQRRLAPPSGQCRQDG
                     "
     gene            10469..11563
                     /locus_tag="VFF98_01585"
     CDS             10469..11563
                     /locus_tag="VFF98_01585"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU62348.1"
                     /translation="MNRNLSIFLDICRLSAALLVFIDHARNLGLWPFFRAIAGIGSEA
                     VAVFFVLSGFIIRFVSKEKHESNFFNYTVARLSRVYPVIIISITIGYLSDFVGIFYAK
                     DSYSDIANMHNGTLSEALADITFTNELWFSHTVFGSNGPYWSMGFEVPYYIVFAFISF
                     TQARLRMVLVAGWAALVGPKILLYFTIWLLGVALYSVIQQMRTRRVALGLGIMLAMLV
                     LFSIYTFILHPMKWIYQNAYEAHSLGSLLPPAMYYAGIGIMFAVIVVGFQWASLGMNL
                     FSPAFERLVRWLAGGSFTVYLVHLPVMHMVLALNPAMKNTRWPAIAGMALVLAICFLA
                     AELGERRKYFYRGLIEAIANRFTRPLREGR"
     gene            complement(11590..11775)
                     /locus_tag="VFF98_01590"
     CDS             complement(11590..11775)
                     /locus_tag="VFF98_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62349.1"
                     /translation="MIAGFAPGDIVLQCGSGVTACHNALAMEIAGLGGARLYPGSWSE
                     WIGDPARPVAVGAGTGA"
     gene            complement(11892..13028)
                     /locus_tag="VFF98_01595"
     CDS             complement(11892..13028)
                     /locus_tag="VFF98_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HZU62350.1"
                     /translation="MQRHTLAPRAGWEQWVEALGLLWHTADGVPYWSESAFYSFSAAE
                     IDRIAEASEALYRLFLAAGQYVIEQRLLAAFGIPDWCVPLIEAAWESQPPALAYGRFD
                     LGLDRDGTPRLFEFNCDTPTSLVEAAVIQWFWKEEVFPAQDQFNRLHEALVARWRAIA
                     PRLPAGPVHFAHVPDRLGEDTLTTAYMMDLAGEAGLATCRLTMRDIGWKSGPGGGFVD
                     LEGAPIGVLCKLYPWEWLVAEPFGRHIADGLCGTLWIEPVWKMIWSNKAILPVLWQLF
                     PGHPNLLWAGHAAPAGAYVRKPLLGREGGNVTIVKDGAVLAANGGRYTGPVVYQAFCD
                     LPGAGGHYPVLGSWVVDGEAVGLGVREGGRITTNRDRFVPHVIS"
     gene            complement(13036..13410)
                     /locus_tag="VFF98_01600"
     CDS             complement(13036..13410)
                     /locus_tag="VFF98_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62351.1"
                     /translation="MSRPIDRNALIALVGLSAGMLAGCGPSDAGQWRVCTDDRGRRVA
                     DADCAPGSTGGGHGFGGSGGGGWRYLSREGAAPAIGDEVSGGTRAPSGGTAEAAPAEG
                     ITRGGFGGMGEGGEGGHGGAGE"
     gene            complement(13500..>13873)
                     /locus_tag="VFF98_01605"
     CDS             complement(13500..>13873)
                     /locus_tag="VFF98_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62352.1"
                     /translation="PAYASSLSQTLPNVARGGIRGEARWNGQWRGRWPLSTGASLRYI
                     GRSVLGVGADQGMTQGGYVVLGANARIQIGRLGLSLLADNLGNVRANTFSFGNPFGLA
                     QGNQITPLRPRTMRFGLDAQF"
BASE COUNT         2261 a         4743 c         4376 g         2493 t
ORIGIN      
        1 gcggggctgg cagcgctcgt ttttgccggg ctggtccggt ggctgtggcc aaccgccccc
       61 gccctgctgg ccagcgccat cgttggcggc atgggcattg ccgtggtgca ggcgctgatg
      121 cccgcgctca tccggcaccg ggcaggcgcg caggcgccgg cgctgatggg cttctattcc
      181 accgcgatca tgggcggcgc ggcgctggcc agctttgcca caccgcgcct tgcgctggcg
      241 cagggctggg ccgtggcggc gggcatctgg gcgctgcccg ccctcatcgg cctggtgatc
      301 tggtggctgg cgacggacaa tagcgacgag gccgcgcccg cgccgccgca aagcccggtc
      361 tcgcgcagcc gcagaagctg gctgctgctg tccttcttcg gcctgggtac ggggggctat
      421 acgctcgtgc tcgcctggtt gccgccctat tatactggcc tcggctggtc ggcgcaggat
      481 gccggcaccc tgctgtccgc cctcacgctg gccgaagtgg cggcgggcat cggcatcgcc
      541 atggccgtcg gccggttcgc agaccggcgt gcgcttatcc tgctggccat tggcgctctg
      601 gtggccgggc tgctctgcct ttgtctggcg ccgctggctc tggcgtggtg cgcggcgctg
      661 ctctgcggcc tgggcatcgg cgcgctgttt ccgctcggcc tgatcgtcgc catggaccat
      721 ggcagggatg ccgcgcaagc cgggcagatc gccggcttcg tgcagggcgg cggctatgtg
      781 ctggcggccc tcctgccgct gatcgccggc gtgctgcgcc agcagctgtc cgatctggcc
      841 ccggcatggt ggctgatggc cgccctgtgc cttgtgcttg cacttttggc catccggctg
      901 cgccccggcg cgtatctcac ctgaaccctg aaaaggaaga cgatcatggc cttttacacc
      961 gtgcaggaac gcagcaatta ctatgtcagc atcggcgaag ggcggcccgt cctgctgctc
     1021 cacggcatca gcaattcggg ccgggcctgg gcgccgcagg tgcccgcgct ggtggcagcc
     1081 ggctatcgcg tgatcgtgcc ggaccatgcc ggccatggcg cttcggccgc cctgtcggcg
     1141 ccgtttggcg tgcaggacat cgccgatgat acagaggcgc tgatcggcca attgggcatc
     1201 gaccggttcg atctcatcgg cctgtcgctg ggcgggatgg tggcgctcga actggcgctg
     1261 cgccacccct cgcgcattgg ccggctgatc gcggcgaaca gtttcgaccg gaccgcaacg
     1321 ccagcctttg ccgccatggc cgcagaatgg gcccgcctgt tccggcagcc ccatggcccg
     1381 gttgtgcggc tggagcagaa ctggccctcg ctggtctctc caacctttca gaaaacggcg
     1441 gacgggctgc gcacctggca ggtgtggcac ggcattgccg ccagcgccga tggcccctcg
     1501 ctggcccatg tcgcgcgtgg gatcaccgat ttcgaccgcg cggatcgcat cggcgagctg
     1561 gccatgccgg ccctgttcat cgccggcggg ctggatcgca tgtcgccgcc cgccctcagc
     1621 cgcgccatgg ccgaacaggc gccccgcggc gcctatcaac tgatcgaggg cgccgcgcat
     1681 atctccaatg tggacagcgc cgacacgttc acgcagagca tgctgcgctt tctggacccg
     1741 gcctttctgg acccggctga gcccaccgcc gccgatcagc cattgtcggg cagcagcaga
     1801 ggcggcatgc tgggcgcatg agcgcacctc tctttctcat ggcatccggg ctgcttgcag
     1861 gcagtatgaa cgccctggcc ggcggcggct ccttcgtcac cctgccggcg ctgatcgcca
     1921 gcggtgtccc ttcagtcatg gccaatgcga ccagcagcgt ggccctctat ccgggcgggg
     1981 tggccagtgc ctgggtgtat cgccacgggc tgggcccggt gtgccgggtg cccctgccgc
     2041 tgatgctggc ggtcaccctg tcgggcggcc ttgccggcgc gctgctgctg ctgtggaccc
     2101 ctggcgcggt gttcgaccgg gtgcttccgc tgctgctgct gctggcaacc ctgatgctgg
     2161 tcttcgcccc gaaggccggt ggttggctgc gcgatcatgt caccatcggc cggccgatga
     2221 tcctgcccat gcagttcgtg ctgggccttt atggcggcta tttcggcggt gccgtcgggc
     2281 tgatgatgat tgcggcatgg agcctgcttc agggcgccga tatcaaggcg ctcaatgcgc
     2341 cgcgcaccct gatggtcacc gcggccaaca ccgtggcgat cgcctgtttc gcgatcgccg
     2401 gtgcggtggc ctggcgctca gcgctgctgg ttggcctggg cgcggtggcg ggcggttatg
     2461 ccggagcgca tctgggccgc cggctttccc ctgcccttgt gcgcatggca acactggcgc
     2521 tgtctgccct ggtgacagcc tcgttcttta tccgggccta tgcctgaagc aggtggccac
     2581 gcaaaggcct caaataccca tcggccaccg gtttcaattt gccccggccg ccccatatga
     2641 tctgccatga ccagcaggat catccacacc cccgatgcca ccgccctgga gcgcctggcg
     2701 cacaaggccg tcaaggcgct tcccgcggtg ttccgcgatc atctggggga tgtggtgttc
     2761 cgcgttcagg attttgccga aagcgccatg ttgcaggccg tcggcctctc cgatccgtgg
     2821 gcgctgctgg ggctctacca tggccgcccc ttcggccagc actcgatctg gtcgaccggc
     2881 gagatgccca gcgagatctt cctgttccgc cgcccgctgc tggaggcctg ccaccagcgc
     2941 ggcgtgccgg tggaagagat ggtcacccat gtgatcgtcc acgaggtggg ccaccatttc
     3001 ggcctctccg atgcggacat gcaggccatc gaggacgagc cctagccatg ggcgaaccgc
     3061 ccgccagcat caaggcttgc gcggcgcagc accccggttg tacgggagcg aaccgcccgt
     3121 cgagtcggtg tttgcgcgga tgctgatccg ggcaagggca tcgccaggac aggcatcggg
     3181 ggttgatctc tgccaccttg caggtttcag cctgagcccg tgttttgagg ccatgcgcct
     3241 cgcgatagac agtgtcaggt aatagccatg gaatgggttc cggtagtctt cattacgttc
     3301 aagctgatcg tgttcggcac tggcatgttc ttcgccatca agtggcatta cgatcaggac
     3361 aagaagaaga aaaacgaagc cagcaagcag caggcaccgg ccgaacagac atctgacgcc
     3421 gcggagtgat cgacgcctgg ctgaagtgct gaacggcggc cagcgaacct cacctgttgt
     3481 tcctgcctag gaacacgggc agaccgctgg ctggcaagcc atgcccgcct caggccctca
     3541 gcaccgccgc ggtgagcgag gcggagagca ccatcaggca gatcgaggcc agccgctgga
     3601 tcaggcgcgt gcgggccggc gagccatggc ggccggccag cgccccgccg ccgctccaca
     3661 ccagcgccac cagcggcacc accaggaaga acagcgccag ccggggcagg aaatcgggcc
     3721 ggcccggcgc cgggatcagg atcaggccca caaccagcgc cttggggtta agcagcgtcg
     3781 tcaggccaac gctgcggcgc gttacccggg ccgggccgcc ctgccgcccc ggcgcccgcc
     3841 acagccgcac ggccagatag agcacccaga ccgccgccgc ccccttcagc gccggcgccg
     3901 ccaggggcca gcgcgcaagg ctggtcgccc ccagccaggc cagcagcgga accgtggcca
     3961 gatagcccag catttcagcc gggatcagcc gcagcgcccg ctgccacccc tgctgcgccc
     4021 cggccagcgc cagcagcgtg ttggtgggcc ccggcgccat cagcagcgcc agaatggcaa
     4081 acagaaaggt ggcacaggtc atcgcgcaaa atccctgcgg ctgggtgtga agcaggtagc
     4141 cccatggggc gccaggcgcc agtgcgcatt gcgccatgtc agctggcggt gggccctcag
     4201 gcagcacccc gttctgcctg ccacagccaa tcggccacgc gcgccgcgtg attggtgcca
     4261 cctgccgcgc gcaacggttc aatcagatga tagccccgcg cgctggtcag cggggtatcg
     4321 cacaggcgga tcaggctgcc gttggcgatc aactcgtcga tcagcggcgc ccagcccagc
     4381 gccaccccct gccccagcaa aaccgcctgc aacacgatct ggtaattgct gaaaaccagt
     4441 tcctgctggc ggcggggcgg cgtgatgccc atgccggcaa accaatcgtc ccattcaaac
     4501 cagcgctggg cgccgtgatg gcgcaaatgc agcaaacgca gatcggcgat ttgcgccggt
     4561 gccaggggcg cctgcgcata agccggcgca cagaccggat agaccctttc ggcaaacagc
     4621 cgccgcgcgc ggcatcccgg ccaatcgctg ccgccaaaca gcaccgccag atcgaaatcc
     4681 ggctcgccga tatccgatcc gccctgcgag gtgacgaggc gaatttccac ttgcggcatc
     4741 agttcggaaa gctcggcgat gcgcggcatc agccaccagg ccgcaaagcc aaaatcggtg
     4801 aggatattga ccgtgcgccc ggagcctgcc cggcgcgcct cggccacgcc ccgcgccagg
     4861 gtgtgcaggc catctgtggc ggcatccagc agtttggcgc cggcatcggt cagctgcacg
     4921 ccgcgatggc ggcggtgaaa cagcacaacg cccagttcgc tctccaactg ggcgatatgc
     4981 tggctgacgc cggcctgggt catcgccagc tcgccggcgg cggcggcgaa tttgccatgg
     5041 cgcgcagccg cctcgaacac cgcaaggctg cgcaacggca ggcgcccaaa gcgtgctggt
     5101 ataaactcca ctttccctac ccttaagaaa acaccggctt caacgcccct accgaaagct
     5161 ctaatgaaac ccaaccattt cactagagaa tataggttga tgtcagcaaa gcccaacttt
     5221 cttgtcctga tggccgatca gatggccgcc gatgtgctgc ccttcatggc gcaatcgccg
     5281 gtgatcgccc cgcacatggg caagctggcc gcacaaggcg tggtgtttgc cggcgcctgt
     5341 tgccccagcc cgctgtgcgc gccctcgcgc ttttcgctgc tttcggggca actgccctcg
     5401 cgcatcggcg cttatgacaa cgcctgcgaa tttcccgcag aaacccccac catcgcgcat
     5461 tatctgcgcg cgggcggttg gcatacgatc ctgtcgggca agatgcattt cgtcggcccc
     5521 gaccagcttc acggctttga ggaacggctg accaccgata tctaccccgc cgacttcagc
     5581 tggacccccg actggacccg gcccgaccac cgccccagct ggtatcactc gatggattcg
     5641 gtgctgaccg ccggccccac cattcgcacc aaccagatcg actatgacga ggaagtggtg
     5701 ttcaccgccc ggcgcaagct ttacgatctt gcgcgcgcgc gcaacgatcg gcccttcttc
     5761 atggtcgcct cgctgaccca cccgcacgac ccttttgcca ttcagcagcg ctattgggat
     5821 ctttacgagg gcgttgatat cccgatgccg cgcgtgccct ttgatcgcgc ggcgctggat
     5881 ccgcattcgg cccggctgcg ccatgtctgc ggtgccgaca tcgatccggt gcgccccgac
     5941 cagctgcgcg ccgcgcgccg cgcctatttc ggcgcgatca gctttgtcga cgaccaattc
     6001 ggcgccatcc tcgacacgct ggccgaaacc gggctggccg agaacaccat cgttatcgtc
     6061 tgcggcgatc acggcgaaat gctgggcgaa cgcgggctgt ggtacaagat gagcttcttc
     6121 gagggcgccg tgcgggtgcc catggtgatc catgcgccgc agcgctttgc cccccggcgg
     6181 gtggaagcca gcgtgtcgac gctcgacctc ttgccaaccc tgctcgatct tgccggcctg
     6241 cccgctgccg atgccgcgcc ggtcgatggc cgcagcctgc tgccgcatct gcagggcagc
     6301 gccggccccg atgaagccct gggcgaatat ctgggcgaag gggcgctcgc ccccatcgtg
     6361 atgatccgcc gagagggctg gaaatacatc cattcgccca ccgatcccga tcagctctac
     6421 caccttgccg cagatcccga cgagcgcacc aaccttgccg ccgacccggc ccatgcgccc
     6481 cgcatcgccg cccttcgcgc cgaagtgcag gcgcgctggg atctgcccga aatcgccgcc
     6541 gacgtgctgg ccagccagcg ccggcgccgg cttgtcgcca aagccaatgc caccggacaa
     6601 ctgacgcatt gggatcatca accgccgcgc gatgcctcgc gcgcgtacat ccgcgcccac
     6661 atggatctcg aacagctgga agcgcaggcg cgctttccgc gtgtgcgttt cgactgatct
     6721 ggcgcgccga tgccaaaaac cagggggatt tttgtgatga accatgctct tgtccgcgcg
     6781 ctgctgcttg ctggcagcgt ttttccggcc ctgccggcgc atgccgaagc agccgccgac
     6841 acgcccgctg atacagccgc caccggcacc cgggacatcg tcgtcaccgc caccaagcgg
     6901 gccgccaacg cgcaggatgt gccgctgtcg atcagcgtgc tgggcggcca ggcgattgcc
     6961 cgggcacaat tacgcgatgg catcgccatt gcccgcgaaa cgcccaacct tgtggccgaa
     7021 tcggtgatgg gcgccgccat gccgcgctat cgcctgcgcg gcatcggcac gaatgacttt
     7081 gccccaaccg cgacctcggc cgtcggcgtc tatgaggatg aggtctatat ttccgccggt
     7141 tcggcccagt cggaaccgct ttacgacctc gaccgggttg aggtgctgcg cgggccgcaa
     7201 ggctcgctgt ggggcaagaa caccaccgca ggcgcgatcc atttcgttac cgcccagcct
     7261 tccgacacca ccacaggcca gatccgcgca gcctatggca cggataacac gcaggagctg
     7321 gaaggcggca tcggcggaaa actggccgat gggctcgatg cgcgcgtagc cggcgtttac
     7381 aagcatcgca atggccagtt cttcaacgca tattccggcc agcatgtcgg cggctacgac
     7441 atatgggatc tgcgcgggca gttgcgctat cacatcgcgc ccggcgcaac cgttacgctc
     7501 aaggtgcatg gcggccacag ctttcagggc cagccgctgg gccatatcgg cctgctggca
     7561 ggcggcgcgg atgccaccgg ctatgtgcaa agcagcaacc cctcggtgct gtccaacgcc
     7621 ggctcggatt acacaggcgc cgatcggttc ggcgtcgata tgcggcttga tgtcgatctg
     7681 ggcggtgccc ggctgatcga tatcgcggcc tatgaacatt ccagctcgct gatctattcc
     7741 gacgatgccg ccaacccggt caacgcctat gacgagcgct atggtgggca ttccggcacg
     7801 ttcaccaacg aattgcgcct gcaatcggcc gatagcggcg cgttcggctg gatcgccggt
     7861 ttgtattatc tgcgcgacaa gaccgaaagc tttggccagc ttggcctcta tggccctgtc
     7921 aactttggcg tgaatggcga tgcctatgat ttccacgtca ccaccgacaa tgtcgcgggt
     7981 ttcggcagcc tcaactatca gttgaccccg cgcctcaagc tgaccgccgg cgcgcgcttc
     8041 acctgggagc gcaaggccat tgccggcaag gcctatgaat acgtcaccca ggcgggcagc
     8101 gtgtatgatg caagcgtgcc ggggctggtc tatgtgaaca cggccaccaa cacttttgtc
     8161 gacaccacag gcgcggccat tgctgccgtg ccgctcagcc ggtcctggag caaggcgacc
     8221 tgggatgcct cggccgatta ccggatcagc cccggcgccc atgcctttgt ccgcgtggcg
     8281 cgcggtttcc gctcgggcgc gtataacgcc tatgtcgcca atcccggcga tctttcggtg
     8341 tataacccgg aaacgctcac ctcttacgaa ggcggcatca agaccagctg gtgggcgcgc
     8401 aagctcacac tcaatgtgac agccttccac tacaacatca gcaacatgca ggtgaccgtt
     8461 ctgcaatctg tgggtagcac cacgcagaat gcagccgccg cgcgggtgaa cggcttcgaa
     8521 gtcgaggcca gggcccatcc gctggcaggc ctgtcgctgg ggctgggcta tggcttccag
     8581 gcctcgaaat acacaggctt tgccaatgcg tccgtgccct atccgatcaa tcagggcgcc
     8641 ccgctcgatc tgagcggaca aagcctggag cgggcgccca agcacacgct caacctgtcg
     8701 ggcaattatg agcatccgct ggcaggcggc acgctgtttg ccggaaccga ctggcgctat
     8761 accagccgtt atcgcttcca ggtgtggtcg gatgccacca acaccaatcc ggcgccgttc
     8821 ctggcaagcg ctgcaacgca gcagttgctg catgacgcct tttcgcagaa cgacctgtgg
     8881 ctgggcgatg cgcgcatcgg ctggcgggcg gccaatgggc tcgaagtggc ggtctgggtg
     8941 aaaaacctca ccgatcaggt ctacaagacc aacaccttcg gcatgttctt caaccgttcg
     9001 gtcagctact atccgggcga aaggcgcacc gccggcgtct cggcctccta ccggttctga
     9061 gggcggggca tgaacaggcg gcagccatgg ctggctctgg cagcgatcgg cgcggcaacg
     9121 gctgccgccc atatcggcaa caatttcaca acctatctgg tgggcggcct gatcgaccga
     9181 taccacttca gcccgtcgca aatgggcgtt tttgcgatga gcgaaacact ggcctacgcc
     9241 gccgccatgt tcggcgtggc gccgcgcgcg gccagctgca gcccgcgccg gctggccctg
     9301 gcagccagca cgctggtggt ggccgcgcaa gccggatcgg ctgccacctc gggctttgcg
     9361 ctgctgctgg ccggccgggt ggtggcgggt ttgggctttg gcctgatgaa cagcgcggtc
     9421 aatctggccg ccgggcatca ggatcaccct gcccgcgcga tttccaccgg catcatgctg
     9481 caaacgctgc tttacgcgct ggtgaatatc ggcctgccga tgatgggagc ggcccacggt
     9541 gtcgggggca tgtttgccgc gctggccggg ctttcggcgg tgttggggct ggccaccctg
     9601 cggctgccgg acacggcggc gcctgcggcc catggccagg cccgggcggc gcccttgccg
     9661 cgccagggct ggtgggtgct ggcggcgatg gcgctgttct gcgccggcag cctggccatc
     9721 tggcccttca tggagcgtgc cgcgcatgcc attggcctgt cggcggcccg gttcgggcgc
     9781 tatcagtcgc tggcaacgct gctgagcgcc gggggcaatg cgctgctggc gctgctggcg
     9841 gggcggatcg tgcggcgatg ggcgctgaca gccgcgctgc tggcctgcgg gctggcctgc
     9901 gcgggattga ccacgctgac gctccctggc gccttcgccg cggcgctggt ggtgtataat
     9961 gtcagctggt ttctggccta tccgctgttg ctggggctgg cctatgcggc agatcccggc
    10021 gggcgccttg ccgtgatgac caccgccaca tggctgctgg ccgaatccgc agccgcgatg
    10081 ggcgcgggca ctatcgccca aacggccggc tcctatacga tcatcggccc gctggggctg
    10141 gcgggatgcg ttggcgcgct gctgttggca tggccactgg ccagccgcgc cgacgccggc
    10201 cagcgccgcc ttgcgccacc ctctggccaa tgccggcaag atggctgagc gcgaacggcc
    10261 tgggcagccc aggggccatt gcagatggcc cggatcaaaa acaccccatg atggtaaata
    10321 tagtgtttaa catccttatc gttgaataat gcaggttatt tctcaaatta taatgaatca
    10381 tccattaata atgatacaag attttaatgt tgctgcaaaa tttcgtttaa gttagcgacc
    10441 ttgctcggca tgcgagccgg ggggcgctat gaatcggaat ctatccattt tccttgatat
    10501 atgccgtctt tccgctgccc ttctggtatt tatagatcat gccagaaact tgggcctgtg
    10561 gccgtttttc agagcgattg ccgggattgg gtcagaagcc gtcgcagttt ttttcgtttt
    10621 gtccggtttt attatcagat ttgtctctaa agaaaaacat gagtcgaatt tcttcaatta
    10681 cacagtagcc agactttccc gagtttaccc ggttattata atatccataa caattggata
    10741 tttatccgat tttgtcggaa ttttttatgc caaggacagt tattcagata ttgccaatat
    10801 gcataatggc actttgtccg aagcgctggc ggatatcaca ttcactaatg aattgtggtt
    10861 ctctcacacc gtattcggct ccaatggccc ctattggtct atgggtttcg aggtgcctta
    10921 ttatatcgtg tttgccttca tctcttttac ccaggcgcgc ctgaggatgg tgttggtagc
    10981 gggatgggcc gcgctggtgg ggccgaaaat cctgctctat ttcacgatct ggctgctggg
    11041 cgtggcgctc tattccgtca tccagcagat gcgcacgcgc cgggttgcgc tggggctggg
    11101 gattatgctg gcgatgctgg tcctcttctc aatctatact ttcatacttc atccgatgaa
    11161 gtggatttat caaaacgcct atgaggctca cagccttggc agcttgctgc ccccggccat
    11221 gtattatgcc gggatcggga tcatgtttgc ggtcattgtg gtcggcttcc aatgggcttc
    11281 gctggggatg aacctgttct ctccggcgtt tgaacggctc gtgcgctggc tggcgggcgg
    11341 cagtttcacc gtttatctgg tgcatctgcc ggtgatgcac atggtgctgg cgctaaatcc
    11401 ggccatgaaa aacacgcgct ggccagcgat cgccgggatg gcgttggtgc tggccatttg
    11461 ttttctggcc gcggaactgg gcgagcgccg caagtatttc tatcgcggcc tgattgaagc
    11521 catcgccaac cgcttcaccc gccccttgcg cgagggacga tgatggcccg cgccccccgg
    11581 tttacggggt cacgccccgg tgcccgcccc caccgccacc gggcgagccg gatcgccgat
    11641 ccattccgac catgagcccg gatagagccg cgcgccgccc agcccagcga tttccatcgc
    11701 cagcgcatta tggcaagccg tcacgcccga gccgcattgc agcacaatat cccccggcgc
    11761 aaagccggcg atcaccgcgc tgaaatcccc ctgcccgccg agcgcggccg gtgctaacag
    11821 atcctcgtcg agatgcagat aatgcgcgcc gggccgccgg atcgagcgcg gcgccgtgat
    11881 gatcggccca ttcagctgat cacatggggc acaaagcgat cgcggttggt cgtgatgcgg
    11941 ccgccttcgc gcacgcccag ccccactgcc tcgccatcga ccacccagct gcccagcacc
    12001 ggataatgcc cccctgcccc cggcagatcg caaaaggcct gatagaccac cggcccggta
    12061 tagcgcccgc cgttggctgc cagcaccgcg ccatccttca cgatggtcac attgcccccc
    12121 tcgcgcccca gcaggggctt gcgcacatag gcccccgccg gcgccgcatg gccggcccac
    12181 agcagattgg gatggccggg aaacagctgc cacagcaccg gcaggatcgc cttgttgctc
    12241 cagatcatct tccacaccgg ctcgatccac agcgtgccgc acaggccatc ggcaatatgc
    12301 cggccgaagg gttcggccac cagccattcc cacggataga gcttgcacag caccccgatc
    12361 ggcgcgcctt ccagatcgac gaaaccgccg ccgggtccgc tcttccagcc aatgtcgcgc
    12421 atggtgaggc ggcaggtggc cagccccgcc tcgccggcca gatccatcat ataggccgtg
    12481 gtcagcgtgt cctcgcccag ccggtcgggc acatgggcga aatgtacagg gcccgcgggc
    12541 agccgcggcg caatcgcccg ccagcgcgcc accagcgctt cgtggaggcg gttgaactgg
    12601 tcctgcgcgg ggaaaacctc ctccttccag aaccattgga tcaccgccgc ctcgaccagc
    12661 gaggtgggcg tgtcgcaatt gaattcgaag agcctgggcg tgccgtcgcg gtccagtccg
    12721 aggtcgaagc ggccataggc cagcgccggc ggctggcttt cccaggccgc ctcgatcagc
    12781 ggcacacacc agtcgggtat gccgaaagcg gccaagaggc gctgttcgat cacatattgg
    12841 ccagcggcca ggaacaggcg gtacaaggcc tcgctggcct cggcgatgcg gtcgatctcg
    12901 gcggcagaga agctgtagaa ggcgctctcg ctccaataag gcacgccatc ggccgtgtgc
    12961 cacaacaggc ccagcgcttc gacccactgc tcccagccag cccgtggcgc aagggtgtgg
    13021 cgctgcatgg ccggcctatt cgccggcgcc gccgtggccg ccctcgccgc cctcgcccat
    13081 gccgccaaag ccgccgcggg tgatgccttc ggccggggcc gcctctgccg tgccgccaga
    13141 cggcgcgcgc gtgccgccgc tgacctcatc gccgattgca ggcgccgccc cctctcgcga
    13201 gagatagcgc cagccgccgc ccccgctgcc gccgaaacca tgcccaccgc cggtggaacc
    13261 gggcgcgcaa tcggcatcag ccacgcgccg ccccctgtca tcggtgcaca cccgccactg
    13321 ccctgcatca gacggcccgc agcctgccag catcccggcg gaaagcccca caagggcaat
    13381 caacgcattg cgatcaatcg gcctggacat gccatcatcc cccttgccca accggttgat
    13441 agatggcgct tcgcgcccat ggcaagccga ttgtggcccg cccctggcaa agggcggcat
    13501 caaaattgcg catccaggcc aaaccgcatc gtgcgcgggc gcagcggcgt gatctggttg
    13561 ccctgcgcca ggccgaaggg gttgccaaag gagaaggtgt tggcccgcac attgcccaga
    13621 ttgtctgcca gcagcgacag gcccaggcgg ccgatctgga tgcgggcatt ggcccccagc
    13681 accacatagc cgccttgcgt catcccctga tccgcgccca cccccagcac cgaccggccg
    13741 atatagcgca agctggcgcc cgtgctcagc ggccagcgcc cgcgccattg cccgttccac
    13801 cgcgcctcgc cgcggatgcc gccgcgtgcc acattgggca gcgtctgcga caggctggag
    13861 gcataggcgg ggg
//
LOCUS       DASRYE010000029         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_107612,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000029 DASRYE010000000
VERSION     DASRYE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107612"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFF98_01610"
     CDS             <1..213
                     /locus_tag="VFF98_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62353.1"
                     /translation="LEREGLVTRQSDPEDGRQWIYALTAQGQAARERHRALKRGWLTG
                     ALSGLEDSEKEALGQALAVLRRLSSQ"
     gene            210..1454
                     /locus_tag="VFF98_01615"
     CDS             210..1454
                     /locus_tag="VFF98_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62354.1"
                     /translation="MSRMERLHPPEAPATGSLRSLRHYNYRLWFGGALVSNIGTWMQR
                     AAQDWLVLTELTHNDARAVGIVMGLQFGPQFLLMPWTGAAADHMDRRRLLMITQAAMG
                     LLALALGIMTVTGSVRLWQVYAFAAVFGAASAFDAPVRQTFVGDLVGEGDLSNAVALN
                     STIFNGARMVGPVVAGLAIGAVGTGWAFVANGVSFLAVLASLAMLRRDQLHGDARPLR
                     KRGSLFEGFRYVAGRPDLRAISWMLFLIGTFGLNFPIFISTMAVSAFHQNAAGYGLLS
                     SAMAVGSVVAGVLSSRRARPDFGILTASAAGFGITCALAALAPSFWTFGAMLVAVGMT
                     ALTFTNSTNSLMQLTSDPAMRGRVMAIRLAIALGCTPLGAPLVGWVANAWGPRWALGM
                     AALSGFLAMLVGLRHGRRVSLP"
     gene            1486..2235
                     /locus_tag="VFF98_01620"
     CDS             1486..2235
                     /locus_tag="VFF98_01620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU62355.1"
                     /translation="MASETVQIETADGTCTAYVLTPEGAGPWPAVIYYMDAFAIRPAV
                     FDMAAHLTAAGYLVLLPDLFYRHGHYDTLVPKDIFAADQFMTVVRPLMGSTDNRKAGG
                     TDTAAFLAYLDTRADVLGDKVGTVGFCMGGGMAITAAGLFPDRVAAAVSFHGGNLATD
                     LPVSPHLLAPQIKAEVLVAGADNDHSYPLDMAERVEAALSEAGVRHKCEIYAGCAHGW
                     MKPDFPIYDEAGTARGWSEALAVFDRNLPRG"
     gene            2390..2584
                     /locus_tag="VFF98_01625"
     CDS             2390..2584
                     /locus_tag="VFF98_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62356.1"
                     /translation="MIHPHDLPQPSARRLRPVPRAQVKAVSLEAQALPAFWRALPLAI
                     LLSCGMWAAIILAAMLVLHR"
     gene            2640..>3249
                     /locus_tag="VFF98_01630"
     CDS             2640..>3249
                     /locus_tag="VFF98_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62357.1"
                     /translation="MSDTPAPPRPRLIDTLRHPAVGVVAITFLALLVSAGVRSAPGVL
                     MVPLQVHFGWDRATISFTAALGIVLYGLVGPFAAAMMLTIGIRRTMMAGLTLMAAATF
                     ASQWMTQSWQYTLSWGVVSGVGSGAVASVLGAAVVNRWFATHRGLVMGLLSASTATGS
                     LVFLPFLAWLTRHGEWMPVVRTVGIACAVLVPVVAWRVPEHPR"
BASE COUNT          438 a         1090 c         1135 g          586 t
ORIGIN      
        1 ctcgaacgcg aagggctggt cacgcgccag agcgacccgg aggacgggcg gcaatggatc
       61 tacgcgctga cagcgcaggg tcaggccgcg cgcgaacgcc accgcgcgct caagcgcggg
      121 tggttgaccg gcgcgctttc ggggctggag gacagcgaga aggaagcgct ggggcaggcg
      181 ctggccgtgc tgcgccggct aagcagccaa tgagccggat ggagcgcctg cacccgcccg
      241 aggcgcccgc caccggcagc ctgcgttcgc tgcgccatta caactatcgc ctgtggtttg
      301 gcggcgcgct ggtgtcgaac atcggcacgt ggatgcagcg cgcggcgcaa gactggctgg
      361 tgctgaccga actgacgcat aatgacgcgc gggcggtggg cattgtcatg ggcctgcaat
      421 ttggcccgca attcctgctg atgccctgga cgggcgcggc ggccgaccac atggaccgac
      481 ggcgcctgct gatgatcacg caggcggcca tgggcctgct ggcgctggcg ctgggcatca
      541 tgacggttac gggcagcgtg cgcctttggc aggtctatgc ctttgccgcc gtgttcggcg
      601 cggcctcggc ctttgacgcg ccggtgcgcc agaccttcgt tggcgatctg gtgggcgagg
      661 gcgatttgtc gaatgcggtg gcgctcaatt ccaccatctt caatggcgca cgcatggtgg
      721 ggccggttgt ggccgggctg gcgattggcg ctgtgggcac gggctgggcc tttgtggcca
      781 atggcgtgtc gtttctggcg gtgctggcct cgctggccat gctgcggcgc gaccaactgc
      841 acggcgatgc ccgccccttg cgcaaacgcg gcagcctgtt cgagggtttc cgctatgtgg
      901 cgggccggcc agacttgcgg gcgatctcgt ggatgctgtt cctcatcggc acgttcggcc
      961 tcaatttccc catcttcatc tcgaccatgg cggtcagcgc ctttcaccag aacgcggccg
     1021 gctatggcct gctcagcagc gcgatggcgg tgggttcggt ggtggccggc gtgctctcct
     1081 cgcggcgggc gcggcccgat ttcggcattc tcacagcgtc tgccgccggc tttggcatta
     1141 cctgcgcgct ggccgcactc gcgccctcgt tctggacgtt tggcgcgatg ctggtggcgg
     1201 tcggcatgac cgcgctgacc tttaccaaca gcaccaacag cctgatgcag ctcacttccg
     1261 acccggcgat gcgcgggcgg gtgatggcca ttcgcctggc catcgcgctg ggctgcacgc
     1321 cgctgggcgc gccgctggtg ggctgggtgg ccaatgcgtg gggcccgcgc tgggcgctgg
     1381 gcatggccgc gctgtcgggt tttcttgcca tgctggtcgg gctgcggcac ggccggcgcg
     1441 tttccctccc ctgatccccc tctccctccc caaaggacct gtcccatggc atccgaaacc
     1501 gttcagatcg aaaccgccga tggcacctgc accgcctatg tgctcacccc cgagggcgcc
     1561 ggcccctggc ccgcggtcat ctactacatg gatgccttcg ccattcgccc ggcggtgttc
     1621 gacatggccg cgcatctgac ggcggccggc tatctggtgc tgctgcccga cctgttctac
     1681 cgccacggcc actacgacac gctggtgccc aaggatatct tcgcggccga ccagttcatg
     1741 accgtggtgc gcccgctgat gggctcgacc gacaaccgca aggcgggcgg caccgatacc
     1801 gccgctttcc tcgcctatct cgacacgcgc gccgatgtgc tgggcgacaa ggtgggcacg
     1861 gtgggcttct gcatgggcgg cggcatggcc atcacggcag ccggcctgtt ccccgatcgc
     1921 gtcgcggcgg cggtcagctt ccacggcggc aatctggcca ccgacctgcc ggtcagcccg
     1981 catctgttgg ccccgcagat caaggccgaa gtgctggtgg ccggcgccga caacgaccat
     2041 tcctatccgc tcgacatggc cgagcgggtc gaggcggcgc tgagcgaggc gggcgtgcgg
     2101 cacaagtgcg agatctatgc cggctgcgcg catggctgga tgaagccgga cttcccgatc
     2161 tatgacgagg ccggcaccgc gcgcggctgg agcgaggcgc tggcggtgtt cgaccgcaac
     2221 ctgccgcgcg gctgaggcca ggatcgagag tgcccggcag cgctgcgtgg tgctgccggg
     2281 caccttcata aaacgacaaa accactcaca gattgcattt caatcgattt agcaaattag
     2341 tcgcgcaggt ccaataaggc gtcaccaccc cacacaggga agcgccacca tgatccaccc
     2401 gcacgacctg ccccagccct ccgcccgccg cctgcgcccc gtgccgcgcg cgcaggtcaa
     2461 ggccgtcagc ctcgaagctc aggccctgcc cgccttctgg cgcgcgctgc cgctggccat
     2521 cttgctcagc tgcggcatgt gggcggccat cattctggcc gcgatgctgg ttctgcaccg
     2581 ctaatcgcgg ccgtgcccaa ggattgacag tcccgccccg ctcgccccac ccttgggcca
     2641 tgagcgacac acctgccccg ccccgccccc ggctgatcga cacgctgcgc cacccggcgg
     2701 tgggcgttgt ggccatcaca tttctggcgc tgctggtgtc ggccggggtg cgctcggcgc
     2761 cgggcgtact gatggtgccg ctgcaagtgc atttcggctg ggaccgggcg acaatctctt
     2821 tcaccgcggc gctgggcatt gtgctgtatg ggctggtggg gccgtttgcg gcggccatga
     2881 tgctgaccat cggcattcgc cgcacgatga tggccgggct gacgctgatg gcggcggcca
     2941 cctttgccag ccagtggatg acccagagct ggcaatatac gctcagttgg ggcgtggtgt
     3001 cgggcgtggg ctcgggcgcg gtggccagcg ttttgggggc agcggtggtg aaccgctggt
     3061 ttgccacgca tcgcgggctg gtcatggggc tattgtcggc cagcacggcc accggatcgc
     3121 tcgtgttcct gccgtttctg gcatggctga cgcgccatgg cgaatggatg ccggtggtgc
     3181 gcacggtggg cattgcctgc gcggtgctgg tgccggtggt ggcatggcgg gtgcccgaac
     3241 acccgcgcg
//
LOCUS       DASRYE010000030         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_72267,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000030 DASRYE010000000
VERSION     DASRYE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72267"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFF98_01635"
     CDS             <1..642
                     /locus_tag="VFF98_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZU62358.1"
                     /translation="DMATVAAVRPQAIRTIARATDGLVFVKTHNALVAHLGTPMIDMS
                     VTAGAVYVVRNPLDVAISYASFMGVSIDRAIELMNTDGSIIENTDKHAYEYRGSWREH
                     VNSWTRRPARQLHVMRYEDMLEKPLEAFTKLGNFLRLGRTRRQIEAAIAASSFEKLQQ
                     QEEEAGFRERPETMERFFRAGKAGQWRDVLCAAQIDAIVAANAEQMARFGYKP"
     gene            complement(667..1704)
                     /locus_tag="VFF98_01640"
     CDS             complement(667..1704)
                     /locus_tag="VFF98_01640"
                     /inference="COORDINATES: protein motif:HMM:NF017003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HZU62359.1"
                     /translation="MKKTALSLLLASALSTPAFAADADQAAPATPAQEAPVTTPRLNV
                     FVEGTYIFNNNANRLPYAQYFGPNVRGQEYTNAYPYTVGSGKGYEISAGVALRVAPHV
                     SLGLAYTGLRTNQSGDTGMFPDFAINTLLAGGRGRGGNPGIATSYNDAHVKTSVAGDV
                     FDFNAGYDVGLGANEVTLIGGFRYAEFHQNTDAWLYDSEGGLVTNAHRRSRFSGVGPE
                     LGLKGEHSFGPGVISLEGSVLGSLLFGEQRSFTNGAWVDSEGYDPLDSHLVAHSHVAK
                     TLDAEVGLTFHGSRERNHGAALTVGYKVNYYGGVVDTANGSSGDGKEFYGKTSADLLV
                     HGVFVRLNYKM"
     gene            complement(1915..3270)
                     /locus_tag="VFF98_01645"
     CDS             complement(1915..3270)
                     /locus_tag="VFF98_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HZU62360.1"
                     /translation="MPAPSRLPNTPLKAFLRQRRAHLAATLRWAAILLPMAMAVGTLC
                     AAFLWSLDAATRARFDHPWLLYLLPLAGGAVGLLYHLAGRSVEAGNNLIVEQIHEPDG
                     GVPLRMAPLIFIGTVATHLCGGSAGREGTAVQLGGSLASTCARLARLDEAGLSLLLMA
                     GIAAGFGAVFGTPLAGAVFALEVLMVGRMEYGALLPCLVAGIVGDWTCRMWGIEHTAY
                     RIAYLAGHTAVAVEPVLLIKAALAGAAFGLVGLVFAEANHTLGSWLKARVPYGPARPL
                     LGGLVVIALVWLVGTRAYLGLGVWSADPAMPTIAGFFTGPVDHWSWALKLLFTLVTLA
                     SGFKGGEVTPLFFIGAGLGNALGALLGAPVDLFAGLGFVAVFAGAANTPLACTIMGLE
                     LFGAAHMLPIAVACFMAYMCSGHSGIYLSQRVAIPKRAGTALRPGATLRDIRAARPPA
                     R"
     regulatory      complement(3294..3381)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(3488..3562)
                     /locus_tag="VFF98_01650"
     tRNA            complement(3488..3562)
                     /locus_tag="VFF98_01650"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3527..3529),aa:Glu,seq:ctc)
     gene            complement(3658..3733)
                     /locus_tag="VFF98_01655"
     tRNA            complement(3658..3733)
                     /locus_tag="VFF98_01655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3697..3699),aa:Glu,seq:ttc)
     gene            complement(3852..>4288)
                     /locus_tag="VFF98_01660"
     CDS             complement(3852..>4288)
                     /locus_tag="VFF98_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZU62361.1"
                     /translation="PQLDALSAWLDERLAGDVARAQTSQSVTLDLAVAPRGLNPALVE
                     LLESAGPYGMGWPGPRVGVGPVRIVKADVVGADHVRLIVRGADGASFKAMAFRAKETA
                     LGSQLLALPKDRALWLAGRARIDDWGERRQAELHIDDAALAD"
BASE COUNT          786 a         1420 c         1411 g          671 t
ORIGIN      
        1 gacatggcaa ccgtggccgc ggtgcgcccg caggccatcc gcaccatcgc ccgggccacc
       61 gacgggctgg ttttcgtgaa gacccacaac gcgctggtag cccaccttgg cacgccgatg
      121 atcgacatgt cggttaccgc gggcgccgtc tatgtggtgc gcaatccgct ggatgtggcg
      181 atttcctatg ccagcttcat gggcgtttcg atcgaccggg cgatcgagct gatgaacacc
      241 gatggcagca tcatcgagaa caccgacaag cacgcctatg aatatcgcgg atcctggcgc
      301 gagcatgtga acagctggac caggcgcccg gcccgccagc tgcatgtgat gcgctatgag
      361 gatatgctgg aaaagccgct ggaggctttc accaagctgg gcaatttcct gcggctgggg
      421 cgcacgcgcc ggcagatcga ggcggcgatc gcggcctctt cattcgagaa gctgcagcag
      481 caggaggagg aggccggctt tcgcgagcgg cccgagacga tggagcgctt ctttcgcgcc
      541 ggcaaggcgg ggcaatggcg cgatgtgctc tgtgcagcgc agatcgacgc cattgttgcc
      601 gccaatgcag agcagatggc ccggttcggt tacaaaccct aggccatctg ccccgcgggc
      661 aggggattac atcttgtagt tgaggcgcac gaagacgccg tgcacgagca gatcggccga
      721 ggtcttgccg tagaactctt tgccatcgcc cgaggagcca ttggcggtgt ccaccacgcc
      781 gccatagtag ttcaccttgt agcccacggt cagcgcggcg ccatgattgc gctcgcgcga
      841 gccgtggaag gtcaggccca cttcggcatc cagcgtcttg gcgacgtggg aatgggccac
      901 cagatgcgaa tccaggggat catagccttc gctgtcgacc cacgcgccat tggtgaagct
      961 gcgctgctcg ccaaacagca ggctgcccag caccgagcct tcgaggctga tgacgccggg
     1021 gccgaagctg tgttcgccct tcaggcccag ctcgggccca acgccgctga agcgcgagcg
     1081 gcggtgggca ttggttacca gcccgccttc cgaatcatac agccaggcat cggtgttctg
     1141 gtggaattcg gcatagcgga agccgccgat cagcgtcacc tcattggcgc ccaggcccac
     1201 gtcatagccg gcgttgaaat cgaacacgtc gccggccacg ctggtcttca catgggcatc
     1261 gttatacgag gtggcgatac ccgggttgcc gccacggccg cggccgccgg ccagcagggt
     1321 gttgatggca aagtcgggga acatgccggt atcgccgctc tggttggtgc gcaggcccgt
     1381 gtaggccagg cccaggctga catgcggggc cacgcgcagg gccaccccgg cgctgatctc
     1441 ataacccttg cccgagccga ccgtgtaggg ataggcgttg gtgtattcct ggccgcggac
     1501 attcggcccg aaatactgcg cgtaaggcag gcgattggcg ttgttgttga agatgtaggt
     1561 gccttcgacg aagacgttga ggcgcggcgt ggtgaccggc gcttcctgtg ccggggtggc
     1621 cggtgccgcc tgatcggcat cggccgcgaa ggccggcgtc gacagggccg aggccagcag
     1681 caggctcagt gcagtctttt tcatggtacc ccccatatcc tcctggcatc cgcctaacgc
     1741 attataactg catcgaaatg caatttttca cctaaaggtg ggcagatgta tgaactattc
     1801 gacccgaata gagggaatta cgtaattgtc caaggaatgc ccgcgcgtgg catcgatagt
     1861 tcgcctgtgt agcaaatatg caacttgcgg tgtgacatcg ggcaacaagc gcgactagcg
     1921 cgctgggggc cgcgccgccc tgatgtcgcg cagggtggcg ccggggcgca gggcggtgcc
     1981 ggcgcgcttg gggatggcca cgcgctgcga caggtagatg ccgctgtggc ccgagcacat
     2041 ataggccatg aagcaggcca ccgcgatcgg cagcatatgc gccgcgccaa acagctccag
     2101 ccccatgatc gtgcaggcca gcggcgtgtt ggcagcgcct gcaaacaccg ccacaaagcc
     2161 cagcccggca aacagatcca ccggcgcgcc cagcagggcg cccagcgcat tgcccagacc
     2221 tgcgccaatg aagaacagcg gtgtcacctc gccgcccttg aagccgctgg ccagcgttac
     2281 cagcgtgaac agcagcttca gcgcccagct ccagtggtcc accgggccgg tgaagaagcc
     2341 ggcgatggtg ggcatggccg gatcagccga ccacacgccc agccccagat aggcccgcgt
     2401 gcccaccagc cacaccagcg cgatcaccac cagcccgccc agcagcggcc gcgccggccc
     2461 atagggtacg cgcgccttca gccagctgcc cagcgtgtgg ttggcctcgg caaacaccag
     2521 ccccacaagg ccgaaggccg cgcccgccag cgccgccttg atcagcagca ccggctccac
     2581 cgccacggcg gtgtggccgg ccagataggc gatgcgatag gcggtatgtt caatgcccca
     2641 catccggcag gtccagtcgc ccacgatgcc ggccaccagg cagggcagca gcgcgccata
     2701 ttccatgcgc cccaccatca gcacttccag cgcgaacaca gcgcctgcca gcggcgtgcc
     2761 gaacaccgcg ccaaagccgg cggcaatgcc ggccatgagc agcaggctga ggcccgcctc
     2821 atccagccgg gcgaggcggg cgcaggtgct ggccaggctg ccgcccagct gcaccgccgt
     2881 gccctcgcgc ccggccgagc cgccgcacag atgcgtggcc accgtgccga tgaagataag
     2941 gggcgccatg cgcagcggca cgccgccatc gggctcgtgg atctgctcga cgatgaggtt
     3001 gttgcccgct tccacgctgc ggccggccag atgatagagc aggcccaccg caccgcccgc
     3061 cagcggcagc agatagagca gccaaggatg atcgaaacgg gcccgcgtgg ccgcatccag
     3121 gctccacagg aaggccgcgc acagcgtgcc caccgccatc gccatcggca gcagaatggc
     3181 cgcccagcgc aaggtggcgg ccagatgggc gcggcgctgg cgcaggaagg ctttcagcgg
     3241 cgtgttgggc aggcgggatg gggcaggcat gattcctcct tggtgccata tcggcaaagg
     3301 caggaatcat cagccttttg caaggcggtt cggcccatag ggatgccccg ggcccggcgg
     3361 aaatccatcg ccttgatgcg gcctatggcg aaaaggcggc tttgcgtcaa gcctggcagg
     3421 ggatgtggga gggggcggat atccgccttg tgctcttgcc gcctgccacg ccgggccggg
     3481 caagagatgg tgacccctac gagaatcgaa ctcgtgtttc agccgtgaga gggccgcgtc
     3541 ctaaccgcta gacgaagggg ccagcaatgt attcgccacg cctgtgccgc gtgggctttt
     3601 tctcttgatc cccatccccc tttgccgctc cacaagggag ctggcaaagg agagggatgg
     3661 tgacccctac gggaatcgaa cccgtgtttc agccgtgaaa gggccgcgtc ctagaccgct
     3721 agacgaaggg gccgttccag cgtcgccgcc ggaggccgcg catctagggg gaggatcgga
     3781 tatggtcaag cacagatttt gcgggaatgt gaaaaaaatg gaatatcatt taaattcata
     3841 tatttgatcc atcaatccgc cagcgcggcg tcgtctatgt gcaattcggc ctggcggcgc
     3901 tcgccccagt cgtcgatgcg ggcgcggccg gccagccaca gcgcgcggtc tttcggcagg
     3961 gccagcagct gcgatcccag cgccgtctcc ttggcgcgga aggccatcgc cttgaagctg
     4021 gcgccatcgg ccccgcgcac gatcagccgc acatggtctg cgcccaccac atcggccttg
     4081 acgatgcgca ccggccccac gcccacgcgc ggccccggcc agcccatgcc ataggggccg
     4141 gcgctttcca gcagctccac cagcgccggg ttcagcccgc gcggggccac ggccaggtcc
     4201 agcgtcacgc tctggctggt ctgcgcgcgg gcaacatcgc cggccaggcg ctcgtccagc
     4261 caggccgaga gcgcatcgag ctgcggcg
//
LOCUS       DASRYE010000031        17653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_142954,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000031 DASRYE010000000
VERSION     DASRYE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142954"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFF98_01665"
     CDS             complement(<1..699)
                     /locus_tag="VFF98_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HZU62362.1"
                     /translation="MHWVTKPLVMAAMVASGMAQAAAAQANDAWSGDGGVDGFYQWQA
                     PLPARAATLLRKAPAPAQDALPQAAQAWRILYTATDFRDARRLVTVSGLVYVPAGRAP
                     RGGWPVIAWAHGTTGIADVCAPSFTGPSTRDKAYLGAWLARGYAVIASDYAGLGTPGV
                     HPYLQYRSEGLSILDGVSAATRALPQLSARRIAVVGQSQGSQAALSAAELAPAHAPHL
                     GIRAVVATGLIAQED"
     gene            complement(683..2245)
                     /locus_tag="VFF98_01670"
     CDS             complement(683..2245)
                     /locus_tag="VFF98_01670"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZU62363.1"
                     /translation="MRDFQAPGRSAAYARQAMVATSHPDAARVGVEVLRAGGSAADAA
                     IAASAVLAVAEPQNAGLGGDTFYIHADATGRISAYNGSGRTPAALDAAAIGTVGLDSV
                     HSVTIPGAVDALLHLHETHGRLPLDRLLAPAIALAAQGWIVQPRVAWDWQASRQRLAA
                     SPSARAFWGRQVSAGARICQPALARTLRDIAVNGREGFYRGETAQGLVRLLRGLGGAH
                     TMADFAAHAGTPVVPIETTYRGLRVLQCPPNGQGMTVLMLLNLLSGLDMGALWQDQPA
                     FAHSFAQACQLAYAERDRWLADPACAQMPLATLIGQPHAARLRALMNPAAMLALPPPI
                     SEPPHRDTVYLACVDADGQAISFINSLFTDFGSAIHDEASGLLLHNRGASFAPFGDHP
                     NALAPAKRPMHTIIPGMVMEGARPLMPFGVMGGHYQAAGQAFVLSAMLDAGLDPQAAL
                     DLPRHFAFNGEVEMESGVPQAVADALAALGHKVRRAARPIGGGQIIRIDHAQGVLIGG
                     SDPRKDGYALGY"
     gene            complement(2245..2997)
                     /locus_tag="VFF98_01675"
     CDS             complement(2245..2997)
                     /locus_tag="VFF98_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU62364.1"
                     /translation="MNRLARKRILFIGGIGHIGAAAVRAILAEGAQVAVADIDAVAGA
                     AFTATLGEEARFFPVDVASEDSIAALMADATAWLGGLDGLCQNAGFLAAGPTDSFAAD
                     LWDKVYAINVRGQFLGVKHALPHLRAAGGGAIVNMSSLAGKWGGGRRAAYSSAKAAVI
                     GMGRSLAVELAADNIRVNTLCPGWIDTPFNRPAIDEIGGDSAVAAMVAATVPMGRQGR
                     PEEVASSFVHLLSDEASYITGQALIIDGGSYC"
     gene            complement(2994..4322)
                     /locus_tag="VFF98_01680"
     CDS             complement(2994..4322)
                     /locus_tag="VFF98_01680"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU62365.1"
                     /translation="MSPQDNTLLTRIGPGTPMGALMRAYWTPACLSAELVAGGAPMRL
                     MLLGEKLIAFRSPDGSVGIMDHACPHRCASLFYGRNEEGGIRCVYHGWKFAADGQCLE
                     QPNVPVENRFEKRLKARAYRVVEAGGVVWAYLGERETHPPMTAIEATLLDAADVRLTC
                     SLRRCNYLQVLEGEIDTSHFGFLHGGAIDAADLDGTDLNALSLADRAPRYHVGETGAG
                     TMYAAYRDTGVAGETYYRVAHYLLPFYTMFPDGDFAANIVLDAAVPMDDEHTMIFTWL
                     YKGRRTGVRRLKDGSPIPGLERDEAYLPNGTGWFDRWRTVPNADNDYLIDREAQSRRS
                     FTGLTGVQLQDQAVVESMGAIVDRTQENLAMSDLMIATTRRRLLRVLRDHQQGLPAPG
                     VDDPAPFQQARSGAFVASSALGWRTAYARQIALAHNPTGVLHAPTQDFQP"
     gene            complement(4319..6031)
                     /locus_tag="VFF98_01685"
     CDS             complement(4319..6031)
                     /locus_tag="VFF98_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZU62366.1"
                     /translation="MSITNPPALDAITLEILLNALRSVADETYVALMRSAYSTNIKER
                     NDHSTALIDPKGRLIALCESAQPIHLSSMLGLTTRMLETWPQGSFRPGDIFIGNDPYA
                     SGGSHLPDINMSMPVFAGDVLLGFMCTIAHHADVGGMAPGSMSGGTEIYQEGLRIPPV
                     RLFEGGRLCEDLFNLILLNVRVPEERRGDYYAQVAACRLGHRRIVELAEARGADTLLA
                     AFDAIVERTRARMARAAAKLPAGTYHFTDVMDDDGKGAFDMPISVRVTAGAPDGRADG
                     RVIFDFAGTSPAVRGNINCPYYATQSSVAYVFRSMIDPECPINQGMLDAMEVIAPEGC
                     MVNAAFPSAVAGRANTCQRIIDTVIGALAPALPQVAVAAANGANTMAVLSGRGADGQR
                     YVYLETVGGGFGGRATKDGKDGVQVHLINTSNLPVEAIETEYPLMVEAYEFIPDTGGA
                     GHHRGGLGLRRVLRPRGHVAMFSGQGERFRNAPWGVFGGREGGRGRFLIRRADGTEEE
                     LPTKPAFVEIHPDEALIVETAGAGGYGDPALRSPEALAEDRASGKFSAAWLARNYPAQ
                     ETKA"
     gene            complement(6028..8076)
                     /locus_tag="VFF98_01690"
     CDS             complement(6028..8076)
                     /locus_tag="VFF98_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZU62367.1"
                     /translation="MSWFVGIDVGGTFTDFTARNSESGRACVHKRPSTPGNPAEAILA
                     GLDELCAAQGIAPAEISRLSHGTTVATNALIQHRGGKLALIVTKGFRDLLEIGRQVRP
                     LIYDFQADHPPALVPRPRRFEAAERITRDGEVIAPLDEGEIDRLVAQVRAAEVDAVAV
                     CLLFAFVNDGHERRLGAALRKALPGVHVSLSSEVQSEFREYERLSTSVLNAYLQPVMA
                     RYLTDLEAGLAERAPECRLGVNQSSGGLISVERARQFPIRTALSGPAAGISGALDIAR
                     RAGVPDVITLDMGGTSADVALIRDYAADMTRDRWVEGYPVRLSAIDIHAVGAGGGSIA
                     WRDADGLLKVGPQSAGAVPGPACYRRGGTLPTVSDANLVLGRLPAGGLLKGSMAMDVA
                     AARQVIGALAAEIDLPAQATALGIIEIVVANMARAVRKISVERGFDPRDFTLMPFGGA
                     GPLHGVAVARALGIRRLLIPPHPGLLCAQGLLASDDRENFVAPMPMPLGDARLPDALD
                     RAVASLHARAERWFAEEEVLAHRRATDVLLDLRYVGQNFELSVPLALAPGEMPDRAAI
                     AAAFHSAHERTYGFHNGAAPIEVVCMRLVATARLPHAAMAGEVPQPNPALAVGEREVV
                     FAREGSVIAPVYAREALHPGDWFTGPAIVDQLDTTILLHPGDRVRVDATHNLLIEVGK
                     "
     gene            complement(8073..9233)
                     /locus_tag="VFF98_01695"
     CDS             complement(8073..9233)
                     /locus_tag="VFF98_01695"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HZU62368.1"
                     /translation="MPQFLSADLALPPLPATPLPSTPLAAAVPPQARALARASIPGWA
                     GYAPTPLLALPGLAGALGLGAVLAKYEAPRFGLGSFKALGGAFGAMALLAGLDEAQRR
                     AATVVTASDGNHGLSVAWGARRLGCACKIYLHEKVAEARADLIRAQGAQVVRVAGNYD
                     DSTHQAKADAEAFGWYLVSDTAIGPEDAAPGLVMAGYTVMVDEICAQIGQQLPTHVFV
                     QGGCGGLAAAVFGALHEAWGTAARFVIVEPERADCLFQSARAGQPVRVEGDLDTVMGG
                     LSVGEVSLSAWETIRATTRDFLTIADADAIAAMQGLGSGQWGDPPLVAGDAGSAGLAG
                     LMAAAQDSAIRAALGLDAGARVLTIVTEGAVDAEGYQALTGLDPAAVAQVRA"
     gene            9533..10483
                     /locus_tag="VFF98_01700"
     CDS             9533..10483
                     /locus_tag="VFF98_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62369.1"
                     /translation="MELGLKRFNGKISDNDLRLLRTFCTVVRHGGFVAAESELQIGLP
                     SISRYIKDLEIRLGLRLCDRGRRGFSLTPEGRQVHEACTKLFENLDSFETLVRDIHAN
                     PAGSLRIGMVDSLTCHPGFPVAHALRAYKTAFPNVFISLVTKTSNLIEQDVLEGALDI
                     GVVFERRHMEQLQYRHLHEEVSYLYCTEDHPLWRDHGGQIADVDLSVFEFVGYPMVRM
                     MEKLGVGGLFNRTATATNMESIAMLIDSDHYLGFLPEHYLKTLWCADRFRAIAPDTFT
                     FSSSISTITRSGALPPLAAGFLAMLEAVRAGADACPVPAA"
     gene            10567..11880
                     /locus_tag="VFF98_01705"
     CDS             10567..11880
                     /locus_tag="VFF98_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62370.1"
                     /translation="MPDSSPAAVAAAPPSHPAAAATHAPGAWLVLGLLTCAYSLSWMD
                     RFLLVILIDPISRDLHVSNTEIGLLTGFGASLLYSLSGFPMGRLADRHRRTRLVACTL
                     GGWSALTGMIGLAVSFPMLAAARFGMAVLSAGCSPSAYSLIADLFAPVRRGMAIALYS
                     LGISFGTFAGLSLGGIVSDQLGWRQAFVWLSVPGLVLALLLGLVLREPARGRFDGNAH
                     EAHRHYTLREAWQTLAAQPAFLAIAMGFGLLSCAASAFENWIPTWMIRAQHLSTTEVG
                     ALSGVAQGLVGIVASLGFGILADRLAARDRRWYVWIPVLTGGAVLPLVLLFFLLPRGA
                     AYGCYMLIEFLISGCSAPLFAACQMLLPARLRAVGMATVLFLLNTVGMGVGPSLTGFL
                     SDHLPAATPAAALGQAISLMQGVGVLGIVVLLAAARRIAPGAGTP"
     gene            11877..12707
                     /locus_tag="VFF98_01710"
     CDS             11877..12707
                     /locus_tag="VFF98_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU62371.1"
                     /translation="MITARACPASARLVEETLHFLRAAAGCGAAMFYWVEPDLSTTAH
                     ALAGLPQAVPQAYRQEIWRHDPLLTARLAQGRRRLAELEQEACRAPADSAAYRAFLAG
                     HGLAGDVEFLFWAGEESGCAAPWGGISLLPLAGEAPPAARWPQLQAIHRYVDLAMRGH
                     AVARRADAHAFLRHRIGLTRRECDLCDLVAVGAGNADIAALLGLTTATVKSYLRRIFD
                     KMGVDSRTALAVRLSALANGGAGQSDQSAMPDQSAMPDQSAMSERKAATSALPAAALS
                     "
     gene            complement(12644..13534)
                     /locus_tag="VFF98_01715"
     CDS             complement(12644..13534)
                     /locus_tag="VFF98_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZU62372.1"
                     /translation="MDLHRFTRDVDAQTLVHALETDGYAIVENLADVAVMDQIIADMQ
                     PFIDHTAYGYDNYLGKKTRRTGALIARSPASHQLIGHPLVLAACKGVMKKATRFQLQL
                     TQIISVYPGSPAQKLHQDETIWDFFPFPLDYQPQCNLLWALSDYTEGNGATRIVPGSQ
                     DQKGRHYTYEESLPAEMPRGSALFYTGKVFHGAGANRSDRVRQALNVTYAAGWLRQEE
                     NQYLTTPIEVARTLPDDLLKLMGYQMGCLGMGYVRDFEDPLVMVRDDGSVPPLGISLL
                     QESAAAGNADVAAFLSDIAD"
     gene            complement(13650..14633)
                     /locus_tag="VFF98_01720"
     CDS             complement(13650..14633)
                     /locus_tag="VFF98_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZU62373.1"
                     /translation="MPDEDTPLRIGLLLLPGFALMGYAALIEALRGANALSGRQLYQW
                     SHISPDGQAVAASSGARVVADHAVGDRLACDRLFVFAGGDPRGLGDAATCAWLRLLAR
                     HGTVLGGVSGGPYVLARAGLLAGRRATIHWEHRPALREAFPDIALESGLFVIDGPIIT
                     CAGGTAAMDLALALIAGDHTEELALRIGEWFVQAGARAGTGPQRGSLAERYGTTHAAL
                     LKALGWLESHIGDKAPRSAVAEAAGLSLRQLDRLCLAHLGASLAETAMAIRLEAAAHR
                     LRTTDLALADIATDCGFASPAHFSRRFRARHGASPAQWRQQHRAPPPHPPK"
     gene            14726..15979
                     /locus_tag="VFF98_01725"
     CDS             14726..15979
                     /locus_tag="VFF98_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZU62374.1"
                     /translation="MRYSLLQLARQALSGHKGWGPAWRDAAPRAAYDVVIVGGGGHGL
                     ATAYYLAKVHKITNVAVIERGWIGGGNAGRNTTIVRANYALPGNEPFYAFALRLWEGL
                     ERELNYNAMVSQRGVVNLFHTDAQRDAFARRGNAMRLHGVDAQLLDAEGVRKMAPFLA
                     FDNARFPILGGLWHDRAGTARHDAVNWGYARAASALGVDIVQNCAVEGFERDASGAVV
                     GVHTSLGPIRAGRVGLAVAGMSGVVAGLAGLRLPIESHVLQAFVTEGLKPLVPYVMNY
                     GMGHFYISQSDKGGLVFGGGLDGYNSYAQRGGLPVVEDVCEAAMAMMPMVGRARILRS
                     WGGIMDMSMDGSPIIDRTPVPGLYLNAGWCIGGFKATPAAGWAFAHLLARDEPHETAR
                     AYRLDRFRTGHLIDEKGAGAQPNLQ"
     gene            15990..16256
                     /locus_tag="VFF98_01730"
     CDS             15990..16256
                     /locus_tag="VFF98_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZU62375.1"
                     /translation="MRIPCPFCGSRDSREFACRGDAAPRRPAGADSDAEAMFDYVYLR
                     DNPAGPIEEYWYHAQGCRQWLVVHRDTRSHAVLGARFAAAGGRA"
     gene            16253..>17653
                     /locus_tag="VFF98_01735"
     CDS             16253..>17653
                     /locus_tag="VFF98_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU62376.1"
                     /translation="MTQPFRLARGGLIDRGESLRFTFDGRCYTGHPGDTLASALLAHG
                     TRLFGRSFKYHRPRGVLSAGSQEPNALVTLRSGARAEPNTRATTTELFDGLEALSQNR
                     WPSLACDLMAVNQLFAPLLVAGFYYKTFMWPARAWEKLYEPAIRRAAGLGALSGQADP
                     DAYAHDHAFCDVLVVGAGPAGLQAALDAARCGLGVVLADEGGLAGGQLLAARGEGAAW
                     AAAALAELVAMPQVRLLMRTSVFGRFDDGEHGAIERLADHLPAPPAGQPRQRLWKILA
                     RRTIMATGATERPIVFDGNDRPGVMLAHAVSTYLNRYGVAPGRCAVVFTAGDSGWQTA
                     ADLLAAGVALAAVIDARAVVDQALQAPVRAAGARVLLGGQVIAARGSPLRQVIAQGSD
                     GRICRFAADLLAMAGGWNPDIALAAHLGHKPRWREDIAAYGLGEAPAGLAAAGAADGI
                     YGTAGVLASGRRAARQA"
BASE COUNT         2668 a         5945 c         6272 g         2768 t
ORIGIN      
        1 atcctcctgc gcgatcagcc cggtggccac aaccgcgcgg atgcccagat gcggggcatg
       61 ggccggcgcc agttcggccg ccgagagcgc cgcctgcgag ccctggctct gcccgacaac
      121 ggcgatgcgg cgggccgaaa gctgcggcag ggcgcgggtg gcagcgctca ccccgtcgag
      181 gatcgacagg ccttcggagc gatattgcag ataggggtgc acccccggcg tgccaaggcc
      241 ggcataatcg ctggcgatca ccgcatagcc gcgcgccagc caggcgccga gataggcctt
      301 gtcccgcgtg gaagggccgg tgaaagaggg cgcgcagaca tcggcgatgc cggtggtgcc
      361 atgggcccag gcgatcaccg gccagccccc gcgcggcgcc cgccccgccg gcacatagac
      421 cagccccgag accgtgacga ggcggcgcgc atcgcgaaaa tcggtggcgg tgtagaggat
      481 gcgccaggct tgcgcggctt gcggcagggc atcttgcgcc ggggccgggg ccttgcgcag
      541 cagcgtggcg gcgcgcgcgg gcagcggcgc ctgccactgg tagaagccat cgacgccgcc
      601 atcaccggac caggcatcat tggcctgcgc agctgccgcc tgcgccatgc ccgatgccac
      661 catcgccgcc atcacgagag gtttagtaac ccagtgcata tccatccttt ctgggatcgc
      721 ttccgccaat cagaacgccc tgtgcatgat cgatgcgaat gatctgcccg ccgccgatcg
      781 ggcgcgccgc gcgccgcacc ttgtggccca gcgctgccag cgcatcggcc acggcctgtg
      841 gcacgccgct ttccatttcc acctcgccgt tgaaggcgaa atggcggggc aggtcgaggg
      901 cggcctgcgg gtccagcccg gcatcgagca tggccgacag cacgaaggcc tggcccgctg
      961 cctgataatg cccgcccatc acgccaaagg gcatcaacgg gcgcgcgcct tccatcacca
     1021 tgccgggaat gatcgtgtgc atcggccgct tggccggcgc cagcgcattg ggatggtcgc
     1081 caaagggggc gaagctggcg ccgcgattgt gcagcaacag gcccgaggcc tcgtcgtgga
     1141 tggcgctgcc gaaatcggtg aagagcgagt tgatgaagct gatcgcctgg ccatcggcat
     1201 cgacgcaggc cagatagacg gtgtcgcggt gcggcggctc gctgatcggc ggcggcaggg
     1261 ccagcattgc tgccgggttc atcagcgccc gcagccgcgc cgcatgcggc tggccgatca
     1321 gcgtggccag cggcatttgc gcgcaggcag gatcggccag ccagcggtcg cgttcggcat
     1381 aggccagctg gcaggcctgg gcaaagctgt gggcaaaggc gggctgatcc tgccagagcg
     1441 cgcccatatc cagccccgag aggaggttga gcagcatcag cacggtcatc ccctgcccgt
     1501 tgggcgggca ttgcagcacg cgcaggcccc gataggtggt ctctatcggc accacggggg
     1561 tgccggcatg ggcggcaaaa tcggccatcg tgtgggcgcc gcccaggccg cgcagcaggc
     1621 gcaccagccc ttgcgcggtc tcgccacgat aaaagccctc gcgcccgttc acggcaatgt
     1681 cgcgcagggt tcgggccagg gctggctggc agatgcgggc gccggcgctc acctgccggc
     1741 cccagaaggc gcgggcgctg gggctggcgg caaggcgctg ccggctggcc tgccaatccc
     1801 atgccacgcg cggctgcacg atccagcctt gcgcggccag cgcgatggcc ggcgccagca
     1861 gccgatcgag cggcaggcgg ccatgggttt cgtgcagatg cagcagcgca tcgacagcgc
     1921 cgggaatggt gaccgaatgg acgctgtcca gccccaccgt gccgatggcg gcggcatcca
     1981 gcgcggcggg cgtgcggcca gagccgttgt aggcgctgat ccggccggtg gcatcggcgt
     2041 ggatgtagaa tgtgtcgccg cccagcccgg cgttctgcgg ttcggccacg gctaggacgg
     2101 cgctggcggc aatggcggca tcggccgcgc tgccgcccgc gcgcagcact tccaccccca
     2161 cccgcgcggc atcgggatgg gaggtggcca ccatcgcctg ccgggcatag gcggcagacc
     2221 ggccgggcgc ctggaaatcg cgcatcagca gtaggatccg ccatcgatga tgagcgcctg
     2281 gccggtgatg tagctggcct cgtcggagag gaggtggaca aagctggagg ccacttcctc
     2341 gggccggccc tggcgcccca tcggcacggt ggcggccacc atggcggcca cggcgctgtc
     2401 gccgccgatc tcgtcgatgg cggggcggtt gaagggcgtg tcgatccagc cggggcacag
     2461 cgtgttgacg cggatgttat ccgctgccag ctccaccgcc agcgagcggc ccatgccgat
     2521 caccgccgcc ttggccgagg aataggccgc ccgcctgccg cccccccatt tgcccgccag
     2581 cgacgacatg ttgacgatgg cgccgccgcc tgccgcgcgc agatgcggca gggcatgctt
     2641 cacccccagg aactggccgc gcacattgat ggcatagacc ttgtcccaga ggtctgcggc
     2701 aaagctgtcg gtggggccgg cggccaggaa gccggcgttc tggcacaggc catcaagtcc
     2761 gcccagccag gcggtggcat cggccatcag cgccgcgata ctgtcctcgc tggcgacatc
     2821 gacggggaag aagcgcgcct cctcgcccag cgttgcggtg aaggcggcgc ctgccaccgc
     2881 gtcgatatcg gccacggcca cctgggcgcc ctcggccagg atcgcccgca cggccgccgc
     2941 gccgatatgg ccgatgccgc cgataaacag gatgcgcttg cgggccaggc ggttcatggc
     3001 tggaaatcct gggtgggggc gtgcagcacg ccggtggggt tgtgggccag ggcaatctgc
     3061 cgggcatagg cggtgcgcca gcccagcgcg ctgctggcca caaaggcgcc cgagcgggcc
     3121 tgctggaagg gggcgggatc atccacgccc ggtgctggca ggccctgctg gtgatcgcgc
     3181 agcacgcgca gcaggcggcg gcgggtggtg gcgatcatca gatctgacat cgccagattt
     3241 tcctgcgtgc gatcgacaat ggcgcccatg ctttccacca ccgcctgatc ctgcaactgc
     3301 acgccggtga ggccggtgaa actgcgccgg ctttgcgcct cgcggtcgat cagatagtcg
     3361 ttgtcggcat tgggcacggt gcgccagcgg tcgaaccagc cggtgccgtt gggcagatag
     3421 gcctcgtcgc gctccagccc ggggatgggc gatccgtcct tcaggcggcg cacgccggtg
     3481 cggcggccct tgtagagcca ggtgaagatc atcgtgtgct cgtcatccat cggcacggcg
     3541 gcatcgagca cgatgttggc ggcaaaatcg ccatcgggaa acatcgtgta gaagggcagc
     3601 aggtaatggg ccacgcggta gtaggtctcg cccgccacgc cggtgtcgcg ataggcggca
     3661 tacatcgtgc cggcgcccgt ttcgcccaca tgatagcgcg gcgcccgatc agccagcgag
     3721 agggcgttga gatcggtccc atccagatcg gccgcatcga tcgcgccgcc atgcaggaag
     3781 ccgaaatgcg aggtgtcgat ctcgccttcc agcacctgca gatagttgca gcggcgcagc
     3841 gagcaggtga ggcgcacgtc tgcggcatcg agcagggtgg cctcgatggc ggtcatcggc
     3901 gggtgcgttt cgcgctcgcc cagataggcc cagaccacgc cgcctgcttc caccacgcga
     3961 taggcgcggg ccttcagccg cttctcgaag cggttttcca ccggcacgtt gggctgttcc
     4021 aggcactggc catcggcggc gaatttccac ccgtgataga cgcagcggat gccgccttcc
     4081 tcgttgcggc catagaacag gctggcgcag cggtgggggc aggcatgatc catgatcccc
     4141 acgctgccat cgggcgagcg gaaggcgatc agcttctcgc ccagcagcat cagccgcatc
     4201 ggcgcgccgc ctgccaccag ctcggccgac aggcaggccg gcgtccagta tgcgcgcatc
     4261 agcgcgccca tgggcgtgcc cgggccaatg cgggtgagca atgtgttgtc ttgcgggctc
     4321 atgccttggt ctcctgcgcg ggatagttgc gggccagcca ggcggcgctg aacttgcccg
     4381 aggcgcggtc ttcggccagc gcttcggggc tgcgcagggc cggatcgcca tagccgccag
     4441 cgcctgccgt ctcgacgatc agcgcctcgt cggggtggat ttccacaaag gcgggcttgg
     4501 tgggcagctc ttcctcggtg ccatcggcgc ggcggatgag gaagcggccc cggccgccct
     4561 ccctgccgcc gaacacgccc catggcgcat tgcggaaacg ctcgccctgg ccggaaaaca
     4621 tcgccacatg gccgcgcggg cgcagcaccc ggcgcaggcc gaggccgccg cgatggtgcc
     4681 cggcgccgcc ggtatcgggg atgaactcat aggcttccac catcagcgga tattccgtct
     4741 cgatggcttc caccggcagg ttggaggtgt tgatgagatg gacctgcacg ccatccttgc
     4801 catccttggt ggcgcgcccg ccaaagccgc cgcccacggt ttccagatag acatagcgct
     4861 ggccatcggc cccgcggccc gacagcaccg ccatcgtgtt ggcgccattg gccgcggcca
     4921 ccgccacttg cggcagcgcc ggggccagcg cgccgatcac cgtgtcgatg atgcgctggc
     4981 aggtgttggc gcggcccgcc accgccgagg ggaaggcggc gttcaccatg cagccttccg
     5041 gcgcgatcac ttccatcgca tcgagcatgc cctggttgat cgggcattcg ggatcgatca
     5101 tcgagcggaa gacataggcc accgaggatt gcgtggcata ataggggcag ttgatattgc
     5161 cgcgcacggc aggcgaggtg ccggcaaaat cgaagatcac cctgccatct gcccggccat
     5221 ccggggcgcc cgctgtcacg cgcacgctga tcggcatgtc gaaggcgccc ttgccgtcgt
     5281 cgtccatcac atcggtgaag tgataggtgc cggcgggcag tttggccgcg gcgcgggcca
     5341 tgcgggcgcg ggtgcgttcc acaatggcgt cgaaggcggc cagcagcgtg tcggcacccc
     5401 tggcctcggc cagctcgacg atgcggcgat ggcccaggcg acaggcggcc acctgcgcgt
     5461 aatagtcgcc ccgccgctct tccggcacgc gcacgttaag gaggatgagg ttgaacagat
     5521 cctcgcacag ccgcccgccc tcgaacaggc ggacaggggg gatgcgcagg ccttcctgat
     5581 agatctcggt gccgcccgac atggaaccgg gcgccatgcc gcccacatcg gcgtggtggg
     5641 caatggtgca catgaaaccc agcagcacat cgccggcaaa caccggcatg ctcatgttga
     5701 tatcgggcag gtgcgatccg ccgctggcat agggatcgtt gccgatgaag atatcgccgg
     5761 gccgaaagct gccctgtggc caggtttcca gcatccgcgt ggtcagcccc agcatcgagg
     5821 agaggtggat cggctgggcg ctttcgcaca gggcaatgag gcgccccttg gggtcgatca
     5881 gcgcggtgga atggtcgttg cgctccttga tgttggtcga ataggccgag cgcatcagcg
     5941 ccacataggt ctcatcggcc accgaacgca gcgcgttgag caggatttcc agcgtgatcg
     6001 catccagcgc cggggggttg gtgatgctca tttgcccacc tcgataagca ggttgtgggt
     6061 ggcatccacg cgcacgcggt cgccggggtg gaggaggatg gtggtgtcga gctggtcgac
     6121 gatggcaggg ccggtgaacc agtcgcccgg atgcagcgcc tcgcgcgcgt agacgggggc
     6181 gatcacgctg ccctcgcggg caaacaccac ttcgcgctcg cccacggcca gcgccgggtt
     6241 gggctggggc acttcgccgg ccatggcggc atggggcagc cgggcggtgg ccaccagccg
     6301 catgcagacc acctcgatcg gcgctgcgcc attgtgaaag ccataggtgc gctcatgcgc
     6361 gctgtggaag gccgcggcga tggcggcgcg atcgggcatt tcgccgggcg ccagcgccag
     6421 cggcaccgac agttcgaaat tctggccgac atagcgcaga tccagcagca catcggttgc
     6481 gcgccggtgg gccagcacct cttcctcggc aaaccagcgc tcggcccgcg catgaaggct
     6541 ggccaccgcc cggtccagcg catcgggcag ccgggcatcg cccagcggca tgggcatggg
     6601 cgccacgaaa ttctcgcggt cgtcgctggc cagcagcccc tgcgcgcaca gcaggcccgg
     6661 atgcggcggg atcagcaggc ggcggatgcc cagcgcgcgg gccaccgcca cgccatgcag
     6721 gggcccggcc ccgccaaacg gcatcagcgt gaaatcgcgc gggtcaaagc cgcgttccac
     6781 cgagatcttg cgcaccgcgc gggccatgtt ggccaccacg atctcgatga tgcccagcgc
     6841 cgtggcctgc gccggcagat cgatctcggc cgccagcgcg ccgatcacct gccgggcggc
     6901 ggccacatcc atggccatgc tgcctttcag caggccgccc gccggcagcc gccccagcac
     6961 cagattggca tccgacacgg tgggcagcgt gcccccgcgc cgatagcagg ccggccccgg
     7021 cacggcgccg gcgctttgcg ggcccacttt cagcagccca tcggcatcgc gccaggcaat
     7081 cgagccgcct cctgccccca cggcatggat atcgatcgcc gagaggcgca ccggatagcc
     7141 ttccacccag cggtcgcggg tcatgtcggc ggcatagtcg cggatcagcg cgacatcggc
     7201 cgaggtgccg cccatgtcga gggtgatgac atcgggcacg ccggcccggc gggcaatatc
     7261 cagcgcgccc gaaatgcccg ccgccggccc tgaaagcgcc gtgcggatgg gaaactggcg
     7321 cgcccgctcc accgagatca gcccgcccga cgactggttg acacccagcc ggcattccgg
     7381 cgcgcgttct gccagcccgg cttccagatc ggtgagatag cgggccatca ccggctgcag
     7441 ataggcgttg agcacgctgg tcgagaggcg ttcgtattcg cgaaactcgc tctgcacctc
     7501 gctgctgagc gagacatgca cgcccggcag cgccttgcgc agcgccgcgc caaggcgccg
     7561 ctcatggcca tcgttcacaa aggcgaacag caggcacacc gccaccgcat ccacttcggc
     7621 cgcgcgcacc tgtgccacca gccggtcgat ctcgccttcg tccagcgggg cgatcacctc
     7681 gccatcgcgg gtgatgcgct cggccgcctc gaagcggcgc gggcgcggaa cgagggcggg
     7741 gggatgatct gcctggaaat catagatcag cgggcgcacc tgccggccga tttccagcag
     7801 atcgcgaaag cccttcgtga cgatcagcgc cagcttgccg ccgcggtgct ggatcagcgc
     7861 gttggtggca acggtggtgc cgtgcgaaag gcggctgatt tcggcagggg caatgccttg
     7921 cgcggcgcac agctcgtcca gcccggccag gatggcctcg gccggattgc cgggcgtgga
     7981 ggggcgcttg tgcacgcagg cgcgcccgct ttcgctgttg cgggcggtga aatcggtgaa
     8041 ggtgccgccc acatcaatgc cgacaaacca gctcatgccc gcacctgtgc aaccgcggcc
     8101 ggatccagcc cggtcagcgc ctgatacccc tcggcatcaa ccgcgccttc ggtaacgatg
     8161 gtgagcacgc gggcaccggc atcaaggccc agcgccgcgc ggatggcgga gtcttgcgcg
     8221 gcggccatca ggccggccag cccggcgctg ccggcatcgc ccgccaccag cggcggatcg
     8281 ccccattgcc cgctgcccag cccctgcatc gcggcgatgg catcggcatc ggcaatcgtc
     8341 aggaaatcgc gcgtggtggc gcggatcgtc tcccatgcgc tgagcgagac ctcgcccacc
     8401 gagaggccgc ccatcaccgt gtcgagatcg ccttccaccc gcaccggctg gccggcccgg
     8461 gccgactgga acaggcaatc ggcccgctcc ggctcgacga tgacgaaacg cgccgccgtg
     8521 ccccaggcct cgtgcagcgc gccgaacacc gccgccgcca gcccgccgca gccgccctgc
     8581 acaaacacat gggtgggcag ctgctggccg atttgcgcgc agatctcgtc aaccatcacc
     8641 gtgtagccgg ccatcaccag cccgggggcg gcatcctcgg gcccgatcgc cgtgtcagac
     8701 acgagatacc agccgaaggc ctcggcatcg gccttggcct ggtgggtgga atcatcgtaa
     8761 ttgccggcca cgcgcaccac ctgcgcccct tgcgcgcgga tgagatcggc ccgcgcctcg
     8821 gccactttct cgtgcagata gatcttgcag gcgcagccca gccgccgcgc gccccaggcc
     8881 accgacaggc catggttgcc atccgatgcg gtgaccaccg ttgccgcccg gcgctgcgcc
     8941 tcgtcgagcc cggccagcag cgccatcgcg ccaaacgccc cgcccagcgc cttgaacgag
     9001 cccaggccaa agcgcggggc ctcatatttg gccagcaccg cccccagccc cagcgcgccg
     9061 gcaaggccgg gcagcgccag cagcggcgtg ggcgcatagc ccgcccagcc gggaatggac
     9121 gcccgggcca gcgcccgcgc ctgcgggggc acggcggcgg ccagcggtgt ggaaggcagc
     9181 ggtgttgcgg gcaggggcgg cagggcaaga tcggcggaaa ggaattgggg catggcgctt
     9241 tcgcaatggg gggcggacgg gagtggcggc gcaataagtt tcatgaatag gcaagtattc
     9301 ttcgcggcca atccaagcat tgcagcatcg ggccggccgg cggcgtgcct gtgccggggg
     9361 tgttggcagc ctggctgatt ggccggatat atcgctgtat cccgcctgaa ttaccggccg
     9421 cgtggcaaat gcacaccccc tgcgcaaagc tgcgccatgg cggccagcat ggcaggaaaa
     9481 gcggcttggc aaatgcggca agcggcaata tcaattgcgc agggagtaaa gtatggaact
     9541 tggcctcaag cgcttcaacg gcaagatcag cgacaatgac ctgcgcctgt tgcgcacgtt
     9601 ttgcacggtg gtgcgccatg gcggctttgt ggcggcggaa agcgaattgc agatcggcct
     9661 gccgtcgatc agccgctaca tcaaggatct ggagatccgg ctgggcctgc gcctgtgcga
     9721 tcgcgggcgg cgcggcttca gcctgacgcc agaggggcgc caggtgcatg aggcctgcac
     9781 caaactgttt gaaaacctcg acagtttcga gacgctggtg cgcgatatcc atgccaatcc
     9841 ggccggttcg ctgcgcattg gcatggtcga ttcgctcacc tgccaccccg gctttccggt
     9901 ggcccatgcc ctgcgcgcct ataaaacggc ctttcccaat gtgttcatct cgctggtgac
     9961 gaaaacctcg aacctgatcg agcaggacgt gctggaaggc gcgctcgata tcggcgtggt
    10021 gttcgagcgg cggcatatgg agcaattgca atatcgccac ctccacgagg aagtgagcta
    10081 tctctattgc accgaggacc accccctgtg gcgcgaccat ggcgggcaga tcgccgatgt
    10141 cgatctgtcg gtcttcgaat tcgtcggtta tccgatggtg cggatgatgg aaaagctggg
    10201 cgtgggcggg ttgttcaacc gcacggccac cgccaccaac atggaatcca tcgccatgct
    10261 gatcgattcc gaccattatc tgggcttcct gcccgaacat tacctgaaaa cgctgtggtg
    10321 cgccgatcgc ttccgcgcca tcgcgccaga cacgttcacc ttctccagca gcatttccac
    10381 catcacccgt tccggcgcgc tgccgccgct ggcggccggc ttcctggcca tgctggaagc
    10441 ggtgcgggcg ggcgcggatg cctgcccggt gccggctgcc tgagcgcgcg ccagatggcg
    10501 caggcggcaa gcaaggttgc gcaatgcgcc atcgaaccgg ggcgcaacaa tcgacttgat
    10561 ggcgccatgc ccgattcatc ccctgccgct gtcgctgccg ccccgccgtc ccatcctgcc
    10621 gctgccgcca cccacgcgcc cggcgcctgg ctggtgctgg gcctgctgac ctgcgcctac
    10681 agcctgagct ggatggaccg tttcctgctc gtcatcctga ttgatccgat cagccgcgat
    10741 ctgcatgtct ccaataccga gatcggcctg ttgaccggct ttggcgcgtc cctgctctat
    10801 tcgctgtcgg gctttccgat ggggcggctg gccgatcggc accgccgcac gcggctggtg
    10861 gcctgcacgc tgggcgggtg gagcgcgctg acagggatga tcggcctggc cgtctccttc
    10921 ccgatgctgg cggccgcgcg ctttggcatg gcggtgctgt cggcgggctg ctcgccatcg
    10981 gcctattcgc tgatcgccga cctgttcgcc ccggtgcggc ggggcatggc catcgcgctt
    11041 tattcgctgg gcatttcctt tggcactttt gccggcctgt cgctgggggg catcgtttcg
    11101 gatcagctgg gctggcggca ggcgtttgtc tggctcagcg tgccggggct ggtgctggcg
    11161 ctgctgctgg ggctggtgct gcgcgagccg gcgcgcgggc gtttcgatgg caatgcccat
    11221 gaggcgcacc gccactacac cctgcgcgag gcctggcaga cgctggccgc ccagccggcc
    11281 tttctggcca ttgccatggg ctttgggctg ctctcctgcg cggcatcggc cttcgagaac
    11341 tggatcccca cctggatgat ccgcgcccag catctgagca cgaccgaagt gggcgcgctg
    11401 agcggcgtgg cgcaggggct ggtggggatt gtcgcctcgc tgggctttgg catcctggcc
    11461 gaccggctgg ccgcgcgcga ccggcgctgg tatgtgtgga tcccggtgtt gaccggcggc
    11521 gcggtgctgc cgctggtgct gctgttcttc ctgctgccgc gcggggcggc ctatggctgc
    11581 tacatgctga tcgaatttct catttcgggc tgttcggcgc cgctgttcgc ggcctgccag
    11641 atgctgctgc ccgcgcgcct cagggcggtg ggcatggcca cggtgctgtt cctgctcaac
    11701 acggtgggca tgggggtggg gccctcgctc accggtttcc tcagcgatca tctgcccgcc
    11761 gccacgccgg ccgcagcgct ggggcaggcg atttcgctga tgcagggagt gggggtgctg
    11821 ggcattgtcg tgctgctggc ggcggcgcgg cggatcgccc ctggtgccgg cacgccatga
    11881 tcaccgcgcg cgcctgcccg gcctcggcgc ggctggtgga ggagacgctc cacttcctgc
    11941 gcgcggcggc cggctgcggc gcggcgatgt tctattgggt ggagccggac ctgtcgacca
    12001 ccgcccatgc gctggcgggc ctgccgcagg cggtgccgca ggcctatcgg caggagatct
    12061 ggcgccatga tccgctgttg acggcgcggc tggcgcaagg ccggcgccgc ctggccgagc
    12121 tggagcagga agcctgccgt gccccggccg acagcgcggc ctatcgcgcc tttctggccg
    12181 gccacgggct ggcgggcgat gtggaattcc tgttctgggc cggcgaggag agtggctgtg
    12241 ccgcgccctg gggcgggatc agcctgctgc cgctggcggg cgaggcgccg ccggccgcgc
    12301 gctggccgca gctgcaggcg atccaccgct atgtcgatct ggcgatgcgc ggccatgcgg
    12361 tggcgcgcag ggccgatgcg cacgcctttc tgcgccatcg catcggcctt acccggcgcg
    12421 aatgcgatct gtgcgatctg gtggcggtgg gggcgggcaa tgccgatatc gccgccctgc
    12481 tggggctgac gacggccacc gtcaaatcct atctgcgccg catcttcgac aagatggggg
    12541 tggacagccg cacggcgctg gcggtgcggc tgtcggctct ggccaatggc ggggctggcc
    12601 agtcggatca gtcggcgatg ccggatcagt cggcgatgcc ggatcagtcg gcgatgtcgg
    12661 agaggaaagc ggcgacatcg gcattgcccg cggccgcgct ttcctgaagc aggctgatgc
    12721 ccagcggcgg cacgctgcca tcatcgcgca ccatcaccag cggatcctcg aaatcacgca
    12781 catagcccat gcccaggcac cccatctgat agcccatcag cttgagcaga tcgtcgggca
    12841 gggtgcgggc cacctcgatc ggcgtggtca gatactggtt ttcctcctgc cgcagccagc
    12901 cggcggcata ggttacgttc agcgcctggc gcaccctgtc ggaacggttg gcgccggcgc
    12961 cgtggaacac cttgccggtg tagaacagcg ccgatccgcg cggcatctcg gccggcaggc
    13021 tttcctcata ggtgtagtgg cggcctttct ggtcctgcga tccgggcacg atgcgggtgg
    13081 cgccatttcc ctcggtatag tcgctcagcg cccacagcag gttgcattgc ggctgataat
    13141 cgagcgggaa ggggaagaaa tcccagatcg tctcgtcctg atgcagcttt tgcgcggggc
    13201 tgccgggata gaccgagatg atctgcgtga gctgcagctg gaagcgggtg gcctttttca
    13261 tcacgccctt gcaggcggcc agcaccagcg ggtggccgat cagctgatgc gaggcggggg
    13321 agcgggcgat cagcgcgccg gtgcggcggg tcttcttgcc gaggtaattg tcgtagccat
    13381 aggcggtatg gtcgatgaag ggctgcatgt cggcgatgat ctggtccatc acggccacat
    13441 cggcgaggtt ctcgacaatc gcatagccat cggtttccag cgcgtgaaca agcgtttggg
    13501 catcgacatc acgggtgaag cggtggagat ccatggggca cttcctggtt gaggcaaggc
    13561 ggcgcgtgcg ccgtcggggc tgaggcaaaa cggcgggtgc gccgctgtgg agccaccaag
    13621 gatctgcccg ggcacagggg ccggcaagcc tactttggag ggtgtggcgg gggagcgcgg
    13681 tgttgctggc gccattgcgc ggggctggcg ccatggcggg cgcgaaagcg gcgcgagaaa
    13741 tgggccgggc tggcaaagcc gcaatcggtg gcaatgtctg ccagcgccag atcggtggtg
    13801 cgcaggcgat gggcggccgc ttccaggcgg atggccatgg cggtttcggc aaggctggcg
    13861 cccagatggg ccaggcacag gcggtccagc tggcgcagcg acaggccggc agcctctgcc
    13921 acggccgatc ggggggcctt gtcgccgata tggctttcca gccagcccag cgccttcagc
    13981 agggcggcat gggtggtgcc atagcgttcg gccaggctgc cgcgctgggg cccggtgccg
    14041 gcccgggcgc cggcctgcac aaaccattcg ccaatgcgca gggccagttc ctcggtatga
    14101 tcgcccgcga tcagcgccag cgccagatcc attgccgctg tgcctccggc gcaggtgatg
    14161 atcggcccgt cgatcacaaa cagcccgctt tccagcgcga tatcggggaa ggcctcgcgc
    14221 agggccgggc ggtgttccca gtggatggtg gcgcgccggc cggccagcag cccggcccgc
    14281 gccagcacat agggcccgcc cgaaacgccg cccagcactg tgccatggcg ggccagcagg
    14341 cgcagccagg cgcaagtggc ggcatcgccc agcccgcgcg gatcgccgcc ggcaaagacg
    14401 aacagcctgt cgcaggccag cctgtcgccc acggcatgat cggccaccac ccgcgccccg
    14461 ctggaagcgg ccaccgcctg cccgtcgggc gagatatggc tccactggta cagctggcgg
    14521 cccgacagcg cattggcccc gcgcagcgct tctatcagcg cggcatagcc catcagcgca
    14581 aagccgggca gcagcagcag gccaatccgc aagggcgtgt cttcatcagg catgggcatg
    14641 tcttaacggg ataagcgaga tcggcgcaag ctgcctagac tgggccctgc cgcacgccat
    14701 cggggcgtgg ggagagaggg tggcgatgcg atattccctg ctgcaactgg cccgccaggc
    14761 gctgtccggc cacaagggct ggggcccggc ctggcgcgat gcggccccgc gcgcggccta
    14821 tgatgtggtg atcgtgggcg gcggcgggca tggcctggcg acagcctact acctcgccaa
    14881 ggtgcacaag atcaccaatg tcgcggtgat cgagcggggc tggatcggcg gcggcaatgc
    14941 cgggcgcaac accaccatcg tgcgggccaa ttatgcgctg cccggcaacg agccgttcta
    15001 cgcttttgcg ctgcgcctgt gggaagggct ggagcgcgag ctgaattaca acgcgatggt
    15061 cagccagcgc ggcgttgtga acctgttcca caccgatgcc cagcgcgatg cctttgccag
    15121 gcggggcaat gccatgcggc tgcatggcgt ggatgcgcaa ttgctggatg cagagggcgt
    15181 gcgcaaaatg gcgcccttcc tggctttcga caatgcccgc ttccccattc tgggcggcct
    15241 ctggcacgat cgcgccggca ccgcgcgcca tgatgcggtg aactggggct atgcccgggc
    15301 tgccagcgcg ctgggggtgg atatcgtgca gaactgcgcg gtggagggtt tcgagcgcga
    15361 tgcctcgggg gcggtggtgg gggtgcacac cagccttggc cccattcgcg ccgggcgggt
    15421 ggggctggcg gtggcgggga tgagcggcgt ggtggcgggc cttgcgggcc tgcgcctgcc
    15481 catcgaaagc catgtgttgc aggcctttgt taccgagggg ctgaagccgc ttgtccctta
    15541 tgtgatgaac tatggcatgg gccatttcta catcagccag tccgacaagg gcgggctggt
    15601 gtttggtggc gggctggatg gctataattc ctatgcccag cgcggcggtc tgccggtggt
    15661 ggaggatgtc tgcgaggcgg cgatggcgat gatgccgatg gtgggccgcg cgcgcatcct
    15721 gcgcagctgg ggcggcatca tggacatgtc gatggatggc tcgcccatca tcgaccgcac
    15781 gccggtgccc gggctctatc tcaacgccgg ctggtgcatc ggcggcttca aggcaacgcc
    15841 ggcggcgggc tgggcctttg cccatctgct ggcccgcgac gagccccatg aaacggcgcg
    15901 cgcctatcgc ctcgaccggt tccgcaccgg ccatctgatc gacgaaaagg gcgccggcgc
    15961 ccagcccaac ctgcaatagg agcccagcct tgcgcattcc atgcccgttt tgcggcagcc
    16021 gcgattcccg cgagtttgct tgccgcggcg atgccgcccc ccgccgcccg gccggcgccg
    16081 acagcgatgc agaagcgatg ttcgactacg tctacctgcg cgacaacccc gccgggccca
    16141 tcgaggaata ttggtatcac gcgcaaggct gccgccagtg gctggtggtg caccgcgaca
    16201 cgcgcagcca tgcggtgctg ggcgcccgct ttgccgccgc tggaggccgg gcatgacgca
    16261 gcccttccgc ctggcccgcg gcgggctgat cgacaggggc gaaagcctgc gcttcacttt
    16321 cgacgggcgc tgctacaccg gccatcccgg cgacacgctg gcctccgcgc tgctggcgca
    16381 tggcacgcgc ctgtttggcc gcagcttcaa atatcaccgc ccgcgcgggg tgctgtcggc
    16441 cggcagccag gaacccaacg cgctggtcac cctgcgcagc ggcgcgcgcg ccgagcccaa
    16501 cacgcgggcc accaccaccg aactgttcga cgggctggaa gcgctgagcc agaaccgctg
    16561 gcccagcctt gcctgcgatc tgatggcagt gaaccagctg ttcgccccgc tgctggtggc
    16621 aggcttctac tacaagacct tcatgtggcc ggccagggcc tgggaaaagc tctatgagcc
    16681 ggccatccgc cgggcggcgg ggctgggggc gctgtcgggt caggcagacc ccgatgccta
    16741 tgcccatgac cacgcctttt gcgatgtgct ggtggtgggg gcggggccgg cggggctgca
    16801 ggcggcgctg gatgcggcgc gttgcggcct tggcgtggtg ctggccgatg aaggggggct
    16861 ggctggcggg caattgctgg cggcccgggg cgagggggcg gcctgggcgg cagcggcgct
    16921 ggccgaactg gtcgccatgc cgcaggtgcg cctgctgatg cgcaccagcg ttttcggccg
    16981 cttcgacgat ggcgagcatg gcgcgatcga gcggctggcc gatcacctgc cggcgccgcc
    17041 cgccggccag ccgcgccagc gcctgtggaa gatcctcgcc cggcgcacga tcatggccac
    17101 cggcgccacc gaaaggccga tcgtctttga tggcaacgac cggcccggcg tgatgctggc
    17161 ccatgccgtc tccacctatc tcaaccgcta tggcgtggcg ccggggcgct gcgcggtggt
    17221 gttcaccgcc ggcgacagcg gctggcagac ggcggcggat ctgctggccg ccggcgtggc
    17281 gctggccgcg gtgatcgatg cgcgcgcggt ggtggatcag gcgttgcagg cgccggtgcg
    17341 cgcggcaggt gcccgggtgc tgctgggcgg gcaggtgatc gccgcgcgcg gcagcccctt
    17401 gcgccaggtg atcgcgcagg gcagcgacgg gcgcatctgc cgctttgccg ccgatctgct
    17461 ggcgatggcc ggcggctgga atcccgatat cgcgctggcc gcccatctgg gccacaagcc
    17521 gcggtggcgg gaggatatcg ccgcttatgg gctgggcgag gcgccggccg ggctggcagc
    17581 ggcgggcgcc gcagatggca tctatggcac cgccggcgtg ctggcctcag gccgccgcgc
    17641 cgcgcggcag gcg
//
LOCUS       DASRYE010000032         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_72350,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000032 DASRYE010000000
VERSION     DASRYE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72350"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2611)
                     /locus_tag="VFF98_01740"
     CDS             complement(<1..2611)
                     /locus_tag="VFF98_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZU62377.1"
                     /translation="MEETTGPRPHAPTHKWRWILPLAALGLMLAWLAAGTWLAPVLIR
                     SMATDWVATRLHKQLALGAIHFDPLRFTLDIDRIAIADRAGARPMVALGHLGVGVAPL
                     SGLGGEWRLTELRLDAPAINAVLRRDGSLNLAELIPPAQGGSTGTTALAIDRLAITGG
                     TLSFLDQSLPGSPHARLAPVNLALSDIHTSRGPDGHLAFSATSQKGEQLALHAALGLA
                     PLAARGDFALAGLRADTLAPFLAGQLPVALGSGRADVAGRFDFAHGPAGLKLDSTLSK
                     AELSDVGLAGGLLKGRLHLASLAVEGGEIHLAGPGWAPTRMSLAARGAVLKDARLEAP
                     ALGAGRALRLGEARLEGLHVDLAGHNADLGSLTLAGLDVPLRRAADGSINLMALVPAT
                     AASAPASASTPASPWHAHLAHFALANAAVHVEDRAVQPASRLEASALSLAIEHIDTDL
                     SRPLPLRFALRLNGAATLSGSGTLTPATTAGTLQLALTGLPLATIMPYLAGLPPLDLR
                     SGTLAAAGRLSLAGSGLQGLRFAGSAGLDHLAVYEKGATSPLVAWNALALAGLAYGPQ
                     GVDIASARLQGVAGHIAIMADKSFNFAPLMATPGAPPVAAPPAASGRPAAAAPAPAPA
                     PAPATSAPAMPIRLHRLMLAGGTLDFADYSITPSFAATISALSGQISNITNAPGAIAA
                     INLQGQVIDRYAPVSVTGTADLFHYDRATDIKLTFSNIELPIFNPYSDVYAGYAIAKG
                     KLSTAFTYHIANRALAAEHHIVIDQLQWGQASASKARVPWPVRLATSLLKDRHGVIRL
                     DVPVRGTLDDPTFRLGPIIWKIIGNVLEKAVTAPFRLIGSLFAGAEKAQFVDFAPGSA
                     QLPAGASESLAALA"
     gene            complement(2803..3429)
                     /locus_tag="VFF98_01745"
     CDS             complement(2803..3429)
                     /locus_tag="VFF98_01745"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZU62378.1"
                     /translation="MHFDLARLAAADRYKLLAATITPRPIAWITSQSASGQRNAAPFS
                     FFNMMSADPPILALGLGRRADGTHKDTAANILATGECVIHLVCRADAEAMNRTCIDAP
                     PGTDELALAGLATLPSLAVAPPRIASAPVAMECRLFQAVEAGQTTIVLAEVLHLHIAD
                     RFVDPVRLHVDAPAMDLVARLHGAGWYGHAPEMFQMARPRWADQAGEG"
     gene            complement(3509..4447)
                     /locus_tag="VFF98_01750"
     CDS             complement(3509..4447)
                     /locus_tag="VFF98_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZU62379.1"
                     /translation="MLSVLATVLPIFALIFAGWALRRGAVFGPEASGALNRFVVYLAL
                     PALILRIVVNARWADIWQPAFVAAFGLGAGAIFALTLALRLRRPRHLADAAIDGLNAA
                     YANTGFMGFPLVLAMLGPAALPPTLAATILTVCVLFALALVLIESGLQGEQHPAHMAL
                     KVAGALVRNPLLVAPVLGALCLAAGLHPPAPVETVLDMLGGAASPAALVALGLFLGER
                     RASQRGETAATALLVALKLLAQPLLTWLLAVRVFALPAPQMHAAVLLAALPTGTGPFM
                     LAEFYRREAGLTARVVLLSTLLSLATVTGLCVLWQA"
     gene            4526..>5108
                     /locus_tag="VFF98_01755"
     CDS             4526..>5108
                     /locus_tag="VFF98_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62380.1"
                     /translation="MIEIRQLRAFVTLAETLHFTRAAERLHITQPPLSRQVAGLEQAL
                     GVRLFDRHSRAVRLTPAGTRLLADARGLLAGLDQACRNAQAAARGELGELAVGFMMHA
                     AYSSVPALARRFVADWPEVRLTLREILPSAIVGAVMAGELDAGITFTPPPQRGLASAV
                     LAREGLCLAVPAGHGLAAGGGPVGAADLAGVPLV"
BASE COUNT          776 a         1741 c         1902 g          689 t
ORIGIN      
        1 tggcaagggc cgccaggctt tcgcttgcgc cggccggcaa ttgcgccgat ccgggcgcga
       61 aatcgacgaa ttgcgccttc tcggcgcctg caaacagcga gccgatcagc cggaacggcg
      121 ccgtgaccgc cttttccagc acattgccga tgatcttcca gatgatcggc cccagccgga
      181 aggtgggatc atccagcgtg ccgcgcaccg gcacatccag ccggatcacc ccgtgcctgt
      241 ccttcagcag cgaggtggcc agccgcaccg gccagggcac ccgcgccttg ctggcgctgg
      301 cctggcccca ttgcagctgg tcgatcacga tatggtgttc ggcggccagg gcgcgattgg
      361 cgatgtgata ggtgaatgcg gtggagagct tgcccttggc gatcgcatag ccggcataga
      421 catcggaata ggggttgaag atcggcagtt cgatgttgct gaaggtcagc ttgatgtcgg
      481 tggcgcggtc atagtggaag agatcggccg tgccggtgac gctgaccggc gcgtagcggt
      541 cgatcacctg gccctggagg ttgatggcgg cgatggcgcc gggggcattg gtgatgttgc
      601 tgatctgccc cgacagcgcg ctgatggtag cggcaaagct gggggtgatg gaataatcgg
      661 cgaaatccag cgtgccgccg gccagcatca gccggtggag gcggatgggc atggcgggcg
      721 cgctggtggc aggggctggc gcgggggcag gcgcgggtgc ggcggctgcg ggccggccgc
      781 tggcagccgg cggggcggcc acgggcggcg cgccgggggt ggccatcagc ggggcgaaat
      841 tgaagctctt gtcggccatg atggcgatat ggccggccac gccctgcagc cgggcactgg
      901 cgatatccac cccttgcggc ccataggcaa ggccagccag cgccagcgcg ttccacgcca
      961 ccagcgggct ggtcgcgccc ttttcataca cggccagatg atcgagcccg gcgctgccgg
     1021 caaagcgcag gccctgcaag ccgctgcctg caaggctgag ccgccctgcc gccgccagcg
     1081 tgcccgagcg cagatcaagc ggcggcaggc cggcaagata gggcatgatc gtggccagcg
     1141 gcaggccggt cagcgccagt tgcagcgtgc cggccgtggt ggccggcgta agcgtgccgc
     1201 tgcccgacag cgtagccgcg ccattcagcc gcagggcaaa gcgcagcggc agcgggcgcg
     1261 acaggtcggt gtcgatatgc tcgatggcca gcgaaagggc gctggcttcc agccggctgg
     1321 ccggttgcac ggcacggtct tcgacatgca cggcggcatt ggcgagggcg aaatgggcca
     1381 gatgcgcgtg ccatggagaa gccggggtgc tggcagaagc cggggcggag gcggcggtgg
     1441 cagggaccag cgccatgaga ttgatcgacc cgtcggccgc gcggcgcagc ggcacatcca
     1501 gcccggccag ggtcaggctg ccgagatcgg cgttgtggcc ggcaagatcg acatgcagcc
     1561 cctccagccg cgcctcgccc agccgcaggg cccggccggc gcccagtgcg ggggcttcca
     1621 gccgggcgtc cttcagcact gcgccgcgcg cggccagcga catgcgcgtg ggcgcccagc
     1681 cgggcccggc cagatggatc tcgccgcctt ccaccgccag gctggccaga tgcagccggc
     1741 ccttcagcag gccgccggca aggcccacat cgctcagctc ggccttggac agggtgctat
     1801 cgagcttcag cccggcgggc ccatgggcaa aatcgaagcg gccggccaca tccgcccgcc
     1861 cgctgcccag cgccaccggc agctggccgg ccagaaaggg cgccagcgtg tctgcccgga
     1921 ggccggcgag ggcgaaatcc ccccgcgcgg ccagcggcgc cagccccagc gcggcgtgaa
     1981 gcgccagctg ctcgcccttc tggctggtgg cgctgaaggc caggtggcca tcggggccgc
     2041 ggctggtgtg gatatcgctg agcgccagat tcaccggggc cagccgcgca tgggggctgc
     2101 cgggcaggct ctggtcgagg aaggagagcg tgccgccggt gatcgccagc cggtcgatcg
     2161 ccagcgccgt ggtaccagtg ctgccgccct gtgccggcgg gatcagctcg gccaggttca
     2221 ggctgccatc gcgccgcagc acggcattaa tcgccggggc atccagccgc agctcggtca
     2281 gccgccattc gccgccaagg cccgacagcg gcgccactcc cacccccagg tggcccagcg
     2341 ccaccatcgg ccgcgcgccc gcccgatcgg caatggcgat gcgatcgata tccagcgtga
     2401 agcgcagcgg atcgaagtgg atggcgccca gcgccagctg cttgtggagg cgggtggcca
     2461 cccagtccgt cgccatgctg cggatcagca ccggcgccag ccaggtgccc gccgccagcc
     2521 aggccagcat caggcccagg gcggccagcg gcaggatcca gcgccacttg tgggtgggcg
     2581 cgtgcgggcg ggggccggtc gtttcttcca tcacttcgca gccccttata ccaccgcggc
     2641 cgccggcaga agcaccacct tggccgcatt tgccgcaagc atcgcacgtg gcgcctctgg
     2701 cgctttgcat ggggcgcttc cggcgctttg caacggcgcc tctggcgctt tgccgccaaa
     2761 gattggccgt ttgatgacgg cgccgacgac gacggggccg cctcagccct ccccggcctg
     2821 atctgcccag cgcgggcggg ccatctggaa catctcgggc gcatggccat accagcccgc
     2881 gccgtgcagc cgcgccacca gatccatggc gggcgcatcc acatgcagcc gcaccggatc
     2941 gacgaagcga tcggcgatgt gaaggtgcag cacctctgcc agcacgatgg tcgtctgccc
     3001 ggcttccacc gcctggaaca gccggcattc catcgccacc ggcgccgagg cgatgcgcgg
     3061 cggcgccacg gccagcgagg gcagggtggc cagcccggcg agcgccagtt cgtcggtgcc
     3121 gggcggggca tcgatgcagg tgcggttcat cgcctcggca tcggcccggc agacgaggtg
     3181 gatcacgcat tcgccggtgg ccaggatgtt ggcggcggtg tccttgtggg tgccatcggc
     3241 cctgcggccc aggcccagcg ccaggatcgg cgggtcggcg ctcatcatgt tgaagaagct
     3301 gaagggggcg gcattgcgct ggccgctggc agactggctg gtgatccaag cgatgggccg
     3361 cggggtgatg gtggcggcca gcagcttgta gcggtcggcg gcggcaaggc gggcaaggtc
     3421 gaaatgcatg gggcagatcc ggcgcggttg ggggccggtg ggtgatggcc gatcacgccg
     3481 gttccgtcca gcccgtcagg ccagcccgtc aggcctgcca gagcacgcac aggcccgtca
     3541 ccgtcgccag cgagagcagg gtggacagca gcacgacacg cgccgtcagg ccggcttcgc
     3601 ggcgatagaa ttcggccagc atgaaggggc cggtgccggt gggcagcgcg gccagcagca
     3661 cggcggcatg catctgcggc gccggcaggg caaacacccg caccgccagc agccaggtga
     3721 gcagcggctg ggccagcagc ttcagcgcta ccagcagcgc ggtggcagcg gtttcgccgc
     3781 gctggcttgc acgccgctcg cccaggaaca ggcccagcgc caccagcgcc gccggcgagg
     3841 ccgccccgcc cagcatgtcg agcacggttt ccacaggcgc cggcggatgc agcccggcag
     3901 ccaggcacag cgcccccagc accggcgcca ccagcagcgg gttgcgcacc agcgcgccgg
     3961 ccaccttcag cgccatgtgg gccgggtgct gctcgccctg caggccgctt tcgatcagca
     4021 ccagcgccag cgcaaacagc acgcagacgg tgaggatggt ggccgccaac gttggcggca
     4081 gggcagccgg ccccagcatc gccagcacca gcggaaagcc catgaagccg gtgttggcat
     4141 aggccgcgtt cagcccgtcg atggcggcat cggccaggtg gcgcgggcgg cgcaggcgca
     4201 gcgccagcgt gagcgcgaag atcgccccgg cgccaaggcc aaaggcggcc acgaaagccg
     4261 gctgccagat atcggcccag cgcgcgttga cgacaatccg caggatcagc gccggcagcg
     4321 ccagatagac gacgaagcga ttgagcgcgc cgctggcctc tggcccgaac accgcgccgc
     4381 ggcgcagcgc ccagccggca aaaatcagcg cgaagatcgg cagcacggtg gcaagaacgg
     4441 aaagcatggc aggggctggc ttatccgctc ggtttgatgc actccaatgc ttttatgggc
     4501 cgccattcat gctcaaaagg tattcatgat cgagatccgc cagctgcgcg cctttgtcac
     4561 cctggccgaa acgctgcatt tcacccgcgc ggcagagcgg ctgcatatca cccagccgcc
     4621 gctcagccgg caggtggcgg ggctggagca ggcgctgggg gtgcgcctgt tcgatcgcca
     4681 ttcgcgcgcg gtgcggttga cgccggccgg cacccgcctg ctggccgatg cgcgcgggct
     4741 gctggcgggg ctggatcagg cctgccgcaa tgcgcaggcc gctgcccggg gcgagctggg
     4801 cgagctggcg gtgggcttca tgatgcatgc cgcctacagc agcgtgccgg cgctggcgcg
     4861 gcgctttgtg gcggattggc ccgaggtgcg gctgaccctg cgcgagattc tgccctcggc
     4921 gattgtcggc gcggtgatgg cgggcgagct ggatgccggc atcaccttca ccccgccgcc
     4981 gcagcgcggg ctggccagcg ccgtgctggc gcgcgagggc ctgtgcctgg cggtgccggc
     5041 ggggcatggg ctggcggccg gtggcgggcc ggtgggcgct gcggatctgg cgggcgtgcc
     5101 gctggtcg
//
LOCUS       DASRYE010000033         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_159948,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000033 DASRYE010000000
VERSION     DASRYE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159948"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFF98_01760"
     CDS             <1..417
                     /locus_tag="VFF98_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU62381.1"
                     /translation="YLARHSAPLDSAEHAVITVMEDQLHLDEPKWLARLLPRARPALR
                     TNNRDVQLEAARAGLGLACLPCYTADAASDLQRVASAGFAPPRTVWLGVHADLRQMPR
                     IRALIEVLDAEFRRQKAQLMPPAAAAFTAPASTPPR"
     gene            complement(386..1363)
                     /gene="virB11"
                     /locus_tag="VFF98_01765"
     CDS             complement(386..1363)
                     /gene="virB11"
                     /locus_tag="VFF98_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HZU62382.1"
                     /translation="MAQSYLDLWLAPLRPVLAQAEVTDIFVNRPGEIWVERLGGSVAP
                     QPCPQLTAPLLWQLARQLAAASDQGISRAHPLLSARLEDGARVQVVAPPATRGDLALA
                     IRKHVAQAPALADYVAGGSFAATRLGQGVGERPVLALPPGDAPAEIAAFLAEMVRQRR
                     NILISGGTASGKTTFLNALLREIPPGERLIAIEDTAELAIPHANAVGLVAVRGPLGEA
                     QVSAEDLLRAALRMRPDRILLGEVRGEEAHTFLRAVNTGHPGSLTTIHADTPAGALEQ
                     LALIILQAGGGLSRADILAYARQVVDVIVQLERRGGVRRISEVVLTRAR"
     gene            complement(1363..2106)
                     /locus_tag="VFF98_01770"
     CDS             complement(1363..2106)
                     /locus_tag="VFF98_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HZU62383.1"
                     /translation="MLPPALPSPAPPAEAARPRGGGAGGAALVVDLTSGAGASTGAAA
                     SADAATADDTARATLIRSRATVIPQGAIIAAVLETPLNSDRPGLARAIVGQDVRGFDG
                     ARVLIPRGSRLVGEFKAQGGGTVSRVLVSWTRLIRPDGVAIRIASPAADGLGGAGIPG
                     TVNAHFLDKFASAVLQSALTVGVNVASTLASSGASTIYLGLPSQSSQIGQQLVPTANR
                     AATVKVPAGAQIAVLVAHDLDFSGTPAVR"
BASE COUNT          345 a          889 c          763 g          279 t
ORIGIN      
        1 tatctggccc gccacagcgc gccgctggac agtgccgaac atgccgtcat caccgtgatg
       61 gaagaccagc tgcaccttga cgagcccaaa tggctggccc gcctgctgcc ccgcgcccgc
      121 ccggccctgc gcaccaacaa ccgcgatgtg cagctggaag cagcccgcgc cgggctgggg
      181 ctggcctgcc tgccctgcta caccgccgat gcggcaagcg atctgcagcg ggtagccagc
      241 gccggctttg ccccgccgcg caccgtgtgg ctgggcgtgc atgccgattt gcgccagatg
      301 ccgcgcatcc gcgcgctgat cgaggtgctg gatgccgaat tccgccgcca gaaggcccag
      361 ctgatgcccc cagcagcggc cgccttcacc gcgcccgcgt caacaccacc tcgctgatcc
      421 gccgcacgcc gccccgccgc tccagctgca cgatcacatc caccacctgc cgcgcatagg
      481 ccaggatatc cgcccgcgag aggcccccgc cggcctgcag gatgatcagc gccagctgct
      541 ccagcgcgcc ggccggcgta tcggcatgga tggtggtgag cgagccggga tgcccggtgt
      601 tcaccgcgcg caggaaggtg tgggcctcct cgccgcgcac ctcgcccagc aggatccggt
      661 ctggccgcat gcgcagggcc gcgcgcagca gatcctccgc gctcacctgc gcctcgccca
      721 gcggcccgcg caccgccacc agccccaccg cattggcatg ggggatcgcc agctcggccg
      781 tgtcctcaat ggcgatcagc cgttcgccgg ggggaatctc gcgcagaagg gcgttgagga
      841 aggtggtctt gccgctggcc gtgccgccgg aaatgaggat gttgcggcgc tggcgcacca
      901 tttcggccag gaaagcggca atctcggccg gcgcatcgcc cggcggcagg gccagcaccg
      961 gccgctcgcc caccccctgc cccagccggg tggcggcaaa gctgccgccg gccacataat
     1021 cggccagcgc cggcgcctgc gccacatgtt tgcggatggc cagcgccaga tcgccgcgcg
     1081 tggccggcgg cgccaccacc tgcacccgcg cgccatcctc cagccgggcc gacagcagcg
     1141 gatgggcgcg gctgatgccc tgatcgctgg ccgccgccag ctgccgggcc aattgccaca
     1201 gcagcggcgc ggtcaactgc gggcagggct gcggcgccac gctgccgccc agccgctcca
     1261 cccagatctc gcccgggcgg ttgacgaaga tgtccgtcac ctccgcctgc gccagaaccg
     1321 gccgcagcgg cgccagccac aggtcgagat agctctgcgc catcaacgca ccgccggcgt
     1381 gccggaaaaa tcaaggtcat gcgccaccag cacggcgatt tgcgcgcccg ccggcacttt
     1441 caccgtggcc gcgcgattgg ccgtgggcac cagttgctgg ccgatctggc tgctttgcga
     1501 tggcaggcca agatagatcg tgctggcccc gctgctggcc agggtggagg ccacattcac
     1561 ccccaccgtc aacgccgatt gcagcaccgc gctggcgaat ttgtcgagga aatgggcatt
     1621 gaccgtgccg ggaatgcccg ccccgcccag cccgtccgcc gctggcgagg cgatgcggat
     1681 agccacgcca tccgggcgga tcagccgggt ccagctcacc agcacgcggc ttaccgtgcc
     1741 gcccccttgc gccttgaatt cacccaccag ccggctgccg cgcgggatca acacgcgggc
     1801 gccatcaaac ccgcgcacat cctgcccgac aatcgcccgc gccaggcccg gccgatcgga
     1861 attgagcggg gtttccagca ccgcggcaat gatcgcccct tgcgggatca ccgtggcgcg
     1921 gctgcggatc agcgtggcac gggcggtgtc atccgccgtg gcggcatcgg cgctggccgc
     1981 agcgccggtg ctggcgcccg cgccgctggt aagatccacc accagcgctg ccccgccggc
     2041 cccgccgcca cgcgggcggg ccgcctctgc cgggggggcc ggcgacggca gggctggcgg
     2101 caacatcggc aacaccggcg gcgataaaac cggggccaaa accggagccg gccgcacaac
     2161 catcacccgc ggcgccacag ggccaggcag cgaggccacc ggccgctccg gcggcaagat
     2221 cagcggcggc ggcgcgggcg gcaggggcaa acccgcagcg gccacgccgc cctgcc
//
LOCUS       DASRYE010000034        12154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_213777,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000034 DASRYE010000000
VERSION     DASRYE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213777"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..401
                     /locus_tag="VFF98_01775"
     CDS             117..401
                     /locus_tag="VFF98_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZU62384.1"
                     /translation="MRIDKLLWFLRLCRSRPLAQALVEQGHIRLNGRRVERSSVTVKA
                     GDVLVVPLPAAVRVIELVALPARRGPASEAQSCYRVLDEARLSPVAAGDS"
     gene            457..795
                     /gene="fdxA"
                     /locus_tag="VFF98_01780"
     CDS             457..795
                     /gene="fdxA"
                     /locus_tag="VFF98_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HZU62385.1"
                     /translation="MTYVVTDACIRCKYMDCVEVCPVDCFYEGENMLVINPSECIDCG
                     VCEPECPAEAILPDTESGLEQWQELNAKFSAEWPNITAKKEVPADADEFKGVDGKFEK
                     FFSEKAGSGD"
     gene            1217..1747
                     /locus_tag="VFF98_01785"
     CDS             1217..1747
                     /locus_tag="VFF98_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZU62386.1"
                     /translation="MTAKAHAFDVGDYVVYPKHGVGRVIELLEQEIAGMKLELYVLRF
                     EKERMTLRVPVNKVESIGMRKLSSDKTLREALDTLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLVAIAEVTRDLFRADDQPEQSYSERQIFEGASSRLARELAAMEKTDEPAALRKIL
                     AILNEHAPKYYDAAAI"
     gene            complement(1805..2752)
                     /locus_tag="VFF98_01790"
     CDS             complement(1805..2752)
                     /locus_tag="VFF98_01790"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZU62387.1"
                     /translation="MIENKLPQILAPDEAGVATAARLLAEGELVALPTETVYGLAARA
                     DSDAAVAAIYRAKGRPDFNPLIVHVADAAAAEALAVLDARARALMAAFWPGPLTIVLP
                     LRAGAGLAPAVTAGLPTVALRCPAHAAMRAVLRAAGLPLAAPSANRSGAVSPTRAEHV
                     ATSLAGRIGWILDGGACPDGLESTIIALRADGGWQILRPGPITAEALTALLGPVTAAA
                     PDAGIEAPGQMTSHYAPGKPVRLHAAAAAGDEFLIGFGALAGDFNLSPTGDLAEAAAR
                     LYAALHAAAASDRPRVAVASVPEAGIGAAINDRLRRAAA"
     gene            2806..4548
                     /locus_tag="VFF98_01795"
     CDS             2806..4548
                     /locus_tag="VFF98_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU62388.1"
                     /translation="MSFTPPTQEQLFAMQVSAGLGELATHPRFAHASTDMVEAIAEGI
                     GAFAQGVWAPTDRAGDTQGAQLREGQVHLPDGFAAAYAAFVEAGWGSISAEEAHGGQG
                     LPTTLAAAVLETLGAANMAFTLLPMLTLGAIEALAHHGSPAQQALYLPKLACGQWSGT
                     MNLTEPQAGSDLGPIRTTATPIAEGPHAGKYRLQGTKIFITWGEHGLAENILHLVLAR
                     LPGAPAGSRGISLFLVPKFLVEPDGTLGARNDVRVMRLEHKLGIHASPTCEMGFGENG
                     ACIGELVGRENEGLKAMFTMMNSARVNVGSQGVQVAEAALQKAMAYAAMRVQSARAGA
                     ADKTPVAIIEHPDVRRMLLRMRALTEGARGLLYYTCGQLDRARLGLAGAQARADLLVP
                     LLKAWGTDTGCAVASLGVQVHGGMGFIEETGAAQHYRDARITPIYEGTNGIQAADFVT
                     RKLGMNAGGVLGALLDEMAAEAAGAPTLAALVADCHEVAQWMLASASLDDRLAGSAPF
                     TAMCATAVAGWQLLRQARAAAAEGTAPAIAQGKPVVARYFLSHILPEAQGLKASAMAG
                     ADLLYALDAAALAG"
     gene            4562..5389
                     /locus_tag="VFF98_01800"
     CDS             4562..5389
                     /locus_tag="VFF98_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HZU62389.1"
                     /translation="MTDRDQLLARIADALERLAPPPPPPVDWLAHDAYLWDGRVGHAV
                     ERLEAPALEQLRGIDAQKTVVVENIRRLAQGHAAHDMLLWGARGMGKSAILRASILAA
                     QAQWPGSVALVQVAPDALPSLTQLFGLLRKVQRHFLVFIDDLGFEENDSAAPRALRSW
                     LEGGVEARPAHVRLAVTSNRRAIVARHMAEQDDPINPRDVVDDKLALADRFGLSIGFH
                     NCSQDDYLAIIAGYAADLGLPFEQADALEWARRRGGRSGRVARHYINELAGRAGRQG"
     gene            complement(5419..6915)
                     /locus_tag="VFF98_01805"
     CDS             complement(5419..6915)
                     /locus_tag="VFF98_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZU62390.1"
                     /translation="MNTALRNTLIALGAVALVGGGAFAWVAHGNPSDQSVELFEGARP
                     KLVDPAPESLPSLSLVKPVGWAAGEGPKAAVGLVVTRFAEGLDHPRTMLTLPNGDVLV
                     AETNAPPGNGPGGITGFVQGLLMGFVGAGEASPNRIVLLRDSHGTGQADQRIVLKAQG
                     LQSPSGMAWQDNTLYIADHDAVLAFDYHPGDAQISGNPRKIMDLPGGGNHWMRNLLLS
                     PDGKLLYVAIGSASNIGERGMEAEKGRAAIYEIDLKTGQHRQFAAGLRNPNGLAWNPS
                     TEELWTTVNERDQLGPDLVPDYLTNVPVGAHYGWPWIYWKSKIDDRVDAPMPDYLTDY
                     VRKPEYALGPHVAALGLAFVTGGQKMGSAFANGAFIARHGSWNRKPPSGYDVVFVRFD
                     ANGNPQGLPVPVLTGFLDGKGNTHGRPVWLAWAKDGALLVTDDTAGIIWRVIAPGAAP
                     AATIKPVETDHMKPLHELVDPSKMTPADVLGGGQTGASFDSDQKVRQH"
     gene            complement(6966..7958)
                     /locus_tag="VFF98_01810"
     CDS             complement(6966..7958)
                     /locus_tag="VFF98_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZU62391.1"
                     /translation="MSEEGATGFRIPQPGPMGTGRRKGPALGRGLEALLGESRREEPV
                     VASHGTDAGAPARRDGVTGGLALLAVADIEPHPHQPRRHFDEAALDELAASIAARGVI
                     QPVIVRPFGNGRYQLVAGERRWRAAQRAHVHEVPALVRDLTEREVMALALIENLQRED
                     LNPIEEARAYQRLAETESMTQAEIARMVDKSRSHVANIQRLLMLPEPVIQLVETGDLS
                     MGHARALIGVDNAETLAQSAVGKQLSVREVEALVRKQQRGDAAPQPRRAARDDKHSPE
                     SADIAMMQNQLEEILGLPVKISVDADPRSGAVTIRYRSLDQLDLICQRLTGGGI"
     gene            complement(7955..8740)
                     /locus_tag="VFF98_01815"
     CDS             complement(7955..8740)
                     /locus_tag="VFF98_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU62392.1"
                     /translation="MIRIAIANQKGGVGKTTTAINMATALAANGWRTLLIDLDPQGNA
                     STGVGVGPQARERSSYDLLIGDEPLADCIIETQFQGLDLVPATVDLSGAEVELVGVGE
                     RAERLRDALAKGGPAAGYDICFIDCPPSLGLLTLNALTAADTLLVPLQCEFFALEGLS
                     QLLQTVERVQQRFNADLGIIGVALTMSDKRNRLTDLVADDVRSCLGQLVFETAIPRNV
                     RLSEAPSHGLPALIYDQHCAGSRAYLSLARELIARLPEKRKAA"
     gene            complement(8737..9327)
                     /gene="rsmG"
                     /locus_tag="VFF98_01820"
     CDS             complement(8737..9327)
                     /gene="rsmG"
                     /locus_tag="VFF98_01820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZU62393.1"
                     /translation="MDSAGLARLERFIALLAEENQRQNLVSAASLADVWRRHIADSAQ
                     LLALVPRETSTPWLDLGTGAGLPGIIIAALRPDAEVVMVESRSRRIAWLNHLCAELGL
                     ERASTIGQRLELVQTRHVGVISARAFAPLDRLLTLSARFSTAATTWLLPKGRSAAQEL
                     QGLNGWHHALTVRPSLTDAEAGIISGHVFGKSGVKR"
     gene            complement(9375..11237)
                     /gene="mnmG"
                     /locus_tag="VFF98_01825"
     CDS             complement(9375..11237)
                     /gene="mnmG"
                     /locus_tag="VFF98_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZU62394.1"
                     /translation="MQQFDIIVVGGGHAGVEAAAVAARMGARTALVSFDLDAIGAMSC
                     NPAIGGLGKGHLVREVDAFDGIIGRAADAGAIHYRMLNRSKGSAVQGPRVQADRKRFK
                     AAVQHLLGQQGDLALVQGEVAALRMAGGRVAGVDLADGTALGAGAVVLCTGTFLGGRL
                     FRGEERMVGGRIGEASAQQLAVQLRAADLPMARLKTGTPPRLDGRTIDWARLEEQPSD
                     AEPWSMSAMGGPQRANPQVFCAITRTNAATHEIIRGGLDRSPLFTGAIGAAGPRYCPS
                     IEDKIHRFADRDSHQIFLEPEGLDTPLVYPNGVSTSLPADVQLAMLRSMEGLERVEMV
                     VPGYAVEYDHIDPRALRPSLELRAIPGLYCAGQINGTTGYEEAAAQGLVAGMHATAAV
                     LQRAPAPLDRANAYMAVMIDDLTLQGVSEPYRMLTARAEYRLRLRANNASTRLTPLAM
                     AAGCVGAGRAQWFAQRQQRKAALEQALEREVGAAELSARGLPARSDLGRLPLREWLRF
                     GGVDMAALSPWIDAAHYADADVLEEVAEDATYAPYLARQERELADLRASEALPLGDGF
                     PYGAIAGLSREMVERLTAARPATLAAAGRVRGITPAALAALLVHARRIATGAEG"
     gene            complement(11295..>12154)
                     /locus_tag="VFF98_01830"
     CDS             complement(11295..>12154)
                     /locus_tag="VFF98_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZU62395.1"
                     /translation="ASLAMAGGQLSRQAGAWRDGVLRLSAVVEALLDFGDEDDVPALP
                     EMFHVELSALGDTLSAWLARPRAEVLREGFRVAIAGPPNAGKSTLFNALVESEAAITS
                     PIAGTTRDVLLRSVGLEGVPFQFIDMAGLREEAGDAIEEIGIARARAALEGADLVLWL
                     GPEGEGPGGAWEIEAQVDRAGHVGKAAPRHRVSAMQGEGMAGLVSALAAAARHAMPAP
                     GEAAVNARQHRLLAEAHLALARCQGQGDLLLVAEDLRAARVAFDRLLGRSTTEDMLDT
                     LFGRFCIGK"
BASE COUNT         2016 a         4224 c         3997 g         1917 t
ORIGIN      
        1 cgccgccgca gggccggcag gaccgccccg ccccgccgcc tgccgatgcg gccgcccgcg
       61 cacaggccga aggcaacccc tttggcgtgc tggcgcggct ggtggcctag ggcgcgatgc
      121 ggatcgacaa gctcttgtgg tttctgcgcc tgtgccgcag ccggccgctg gcccaggcgc
      181 tggtggaaca gggccatatc cgcctgaacg gccgccgggt ggagcgcagt tcggtgacag
      241 tgaaagcggg cgatgtgctg gtggtgccgc tgccggcagc ggtgcgggtg atcgagctgg
      301 tggccctgcc cgcccggcgc ggaccggcca gcgaagcgca aagctgctat cgagtgcttg
      361 acgaggcgcg cctttccccc gtagcagcgg gcgattcttg aacgcgccta ccccggcagg
      421 ggcggctgcc attgcatcga aaggggtttt gcagccatga cctatgtcgt caccgacgcc
      481 tgtatccgct gcaaatacat ggattgcgtc gaagtttgcc cggtggattg cttctacgag
      541 ggcgaaaaca tgctggtgat caaccccagc gaatgcatcg attgcggcgt gtgcgagccg
      601 gaatgcccgg ccgaagccat cctgcccgac accgagagcg gtctggagca gtggcaggag
      661 ctgaacgcca agttctcggc ggaatggccg aacatcaccg ccaagaagga ggttccggcc
      721 gacgccgacg aattcaaggg cgtggatggc aagttcgaga agttcttctc ggaaaaggcc
      781 ggctcgggcg attgatcgcc gggcgccggt gcgttgcccg cgccggcttg ccatgatccc
      841 cgcagggccg gcagctgacc ggctttgcgg cgccagtttg cgtttgccag gggtgccctt
      901 cgccaagggg cgcgggcgcc ccggcaggct tttgcgcctt ttgtgatgag gcgcccggcc
      961 aaagcctgat ccttgcccaa aatggcggga ttctaggctc tggcactttt gtcatgagtg
     1021 tgctatataa tagcgcagcc gaaagggttg gtcgcacatc ccgcttggct ccccccaggg
     1081 tttatccctc acaccatgaa aggcggattc gcggcacccg gttgatcttt cgtatcattc
     1141 cgcaagggat gaggctgatc acctgtgttt cgccagggcg cccacgcgtc gaatccgccg
     1201 agcgaaagga cgatagatga ccgccaaggc tcacgccttc gatgttggag attacgtcgt
     1261 ttaccccaag cacggtgtcg gccgggtgat agagctgctg gagcaggaaa tcgccggcat
     1321 gaagctggaa ttgtatgttc tgcgcttcga gaaggaacgc atgacactgc gcgttccggt
     1381 taacaaggtc gaatcgatcg gcatgcgcaa actgtcgtcc gacaagacgc tgcgcgaggc
     1441 gctcgacacg ctgaaaggca agcccaaggt caagcgcacc atgtggtcgc gccgcgcgca
     1501 ggaatatgag gcaaagatca attccggcga tctggtggcg attgccgaag tgacccgcga
     1561 cctgttccgc gccgacgacc agcccgagca gagctattcc gagcgccaga tattcgaagg
     1621 cgcatcgagc cgcctcgcac gtgaactggc cgccatggaa aagaccgacg agccggccgc
     1681 gctgcgcaag atccttgcga tcctgaacga gcacgccccg aaatactacg acgccgccgc
     1741 gatctgatcg cttgcatcgc caggcacgaa aggccgcccc tcacccgggc ggccttttcg
     1801 cgtttcaggc tgccgcgcgg cgcaggcggt cgttgatggc agcgccgatc cccgcttccg
     1861 gcacgctggc aaccgccacg cgcgggcgat cgctggctgc cgctgcgtgg agcgcggcat
     1921 aaagccgggc cgctgcctcg gcgagatcgc cggtgggtga caggttgaaa tcccccgcca
     1981 gcgcgccaaa gccgatcagg aattcatcgc ccgcggccgc tgccgcgtgc agccgcacgg
     2041 gcttgccggg ggcataatgg ctggtcatct ggccgggcgc ctcgatgccg gcatcagggg
     2101 cggcggcggt gaccgggccc aggagagcgg tgagcgcttc ggcggtgatc ggaccggggc
     2161 gcaggatctg ccagccacca tcggcgcgca gggcgatgat ggtggattcc agcccatccg
     2221 ggcaggcacc gccatccaga atccagccga tccgcccggc cagcgaagtg gccacatgct
     2281 ctgcgcgggt ggggctgaca gcgccgctgc gattggccga cggcgccgcc agcggcaggc
     2341 ccgccgcccg cagcaccgcg cgcatggcgg catgggccgg gcagcgcagc gccaccgtgg
     2401 gcaaccccgc agtgaccgcc ggcgccaagc cagcgccggc acgcagtggc agcacgatcg
     2461 tcagcgggcc gggccagaag gcggccatca gcgcccgggc gcgggcatcc agcacggcca
     2521 gcgcttctgc tgctgccgca tcggccacat gcacgatcag cgggttgaaa tcgggccggc
     2581 ccttggcgcg atagatcgcc gccaccgccg catcgctgtc tgcccgggcg gcaaggccat
     2641 agaccgtctc tgtcggcagg gcgaccagct cgccttcggc cagcaggcgg gcggctgtcg
     2701 ccacgcctgc ctcatccggc gccaaaatct gcggtaactt gttctcgatc atggccaagc
     2761 gctataggcc ttgccaccga atgccaagcg aaaggcgcct gatcgatgtc attcaccccc
     2821 cccacccagg aacagctttt tgccatgcag gtgtctgccg ggctgggcga gctggcgacg
     2881 catccgcgct ttgcccatgc cagcaccgat atggtggaag cgattgccga agggatcggc
     2941 gcctttgcac agggcgtgtg ggcgccaacg gaccgggccg gcgatacgca aggggcgcaa
     3001 ttgcgcgagg gccaggtgca tctgcccgat ggctttgccg cggcctatgc ggcgtttgtg
     3061 gaggcgggct ggggctcgat ttcggcggag gaagcgcatg gcgggcaggg cctgcccacc
     3121 acccttgccg ccgctgtgct ggaaacactg ggggcagcca acatggcctt caccctgctg
     3181 ccgatgctga cgctgggcgc gatcgaggcg ctggcccatc atggcagccc ggcccagcag
     3241 gcgctgtatc tgcccaagct cgcctgtggc cagtggtcgg gcacgatgaa cctgacagag
     3301 ccgcaggccg gcagcgatct ggggcccatc cgcaccaccg ccacgccgat tgccgaaggg
     3361 ccccatgccg gcaaataccg gctgcagggc accaagatct tcatcacctg gggcgagcat
     3421 gggctggccg aaaacatcct ccatctggtg ctggcgcgcc tgcccggggc gccggccgga
     3481 tcgcgcggga tctcgctgtt tctggtgccc aaattcctgg tggagccgga tggcacgctg
     3541 ggcgcgcgca acgatgtgcg ggtgatgcgg ctggagcaca agctgggcat ccacgcctcg
     3601 cccacctgcg agatgggctt tggcgagaat ggcgcctgca tcggcgagct ggtgggccgc
     3661 gagaacgagg gcctgaaggc gatgttcacg atgatgaact cggcccgggt caatgtcggc
     3721 agccagggcg tgcaggtggc cgaggcggcc ttgcagaagg ccatggccta tgccgccatg
     3781 cgggtacaat cggcccgggc gggcgcagcg gacaagaccc cggtggcgat catcgagcac
     3841 cccgatgtgc ggcgcatgct gctgcgcatg cgggcgctga cggaaggcgc gcgcggcctg
     3901 ctctactaca cctgcgggca gctggaccgg gcgaggctgg gccttgccgg agcgcaggcc
     3961 cgcgccgacc tgctggtgcc gctgctgaag gcctggggca cggatacggg ctgcgcggtc
     4021 gccagccttg gcgtgcaggt gcatggcggc atgggcttca tcgaggagac gggcgcggcc
     4081 cagcattacc gcgatgcccg catcaccccc atctacgaag gcaccaacgg cattcaggcg
     4141 gccgatttcg tgactcgcaa gctggggatg aacgcgggcg gcgtgctggg cgccctgctg
     4201 gacgagatgg cggcagaggc cgcaggcgcg cccacgctgg cagcgctggt ggccgattgc
     4261 cacgaggtgg cgcagtggat gctggccagc gccagcctgg acgatcgcct ggccggcagc
     4321 gcgcccttca ccgccatgtg cgccacggcc gtggcaggct ggcaattgct gcggcaggcc
     4381 cgggcggcag cggccgaggg cacggcgccg gccattgcgc agggaaagcc ggtggtggcg
     4441 cgctatttcc tcagccatat cctgcccgag gcgcaagggc tgaaggcctc tgccatggcc
     4501 ggcgccgatc ttctctatgc gctggacgcg gcggcgctgg ctggctagct tccgccgcga
     4561 aatgactgat cgcgatcaac ttctcgcccg catcgccgat gcgctggagc gcctggcgcc
     4621 gccccctccc ccgccggtgg attggctggc gcatgatgcc tatctctggg atggccgggt
     4681 tggccatgcg gtggagcggc tggaggcccc ggcgctggag cagctgcgcg gcattgatgc
     4741 gcaaaaaacc gtggtggtgg aaaacatccg ccgcctggcg cagggccatg ccgcgcatga
     4801 catgctgctg tggggcgcgc ggggcatggg caaatcggcg atcctgcgcg cctctattct
     4861 ggcggcgcag gcccaatggc ccggcagcgt ggcgctggtg caagtggcgc ccgatgccct
     4921 gcccagcctg acccagctgt ttggcctgct gcgcaaggtg caacgccatt tcctcgtctt
     4981 catcgacgat cttggctttg aagagaacga cagcgccgcc ccgcgcgccc tgcgcagctg
     5041 gctggaaggc ggcgtggaag cgcggcctgc ccatgtgcgc ctggccgtca cttccaaccg
     5101 ccgggcgatc gtggcgcgcc acatggccga gcaggacgac cccatcaacc cgcgcgacgt
     5161 ggtggacgac aagctggcgc tggccgatcg cttcggcctt tccattggct ttcacaattg
     5221 tagccaggat gattatctgg cgatcattgc cggctatgcg gcagatctgg gcttgccgtt
     5281 cgaacaggcg gacgcgctgg aatgggcccg gcggcgcggc ggccgatcag gccgcgtggc
     5341 gcgccattac atcaacgaac tggcagggcg ggccggccgg caggggtgat cccccctgcc
     5401 cggcggtggc cttatggctt agtgctggcg caccttctgg tctgaatcga aggaggcgcc
     5461 cgtctgcccg ccgcccagca catcggcggg cgtcatcttg ctcggatcca ccagctcgtg
     5521 cagcggcttc atatggtcgg tttcgaccgg cttgatggtg gcagccggtg ccgcgcccgg
     5581 cgcaatcacc cgccagatga tgccggcggt atcatccgtt accaacagcg cgccatcctt
     5641 cgcccaggcc agccacaccg gccggccatg ggtgttgccc ttgccatcga ggaagcccgt
     5701 aagcaccggc accggcaaac cctgcggatt gccattggca tcgaagcgca cgaacaccac
     5761 gtcatagccc gagggcggct tgcggttcca cgaaccatgc cgggcgatga aagcgccatt
     5821 ggcaaaagcg ctgcccatct tctgtccgcc ggtgacgaaa gccagcccca gcgccgccac
     5881 atgcggcccc agcgcatatt cgggcttgcg tacataatcg gtgaggtaat cgggcatcgg
     5941 cgcatccacc cggtcgtcga tcttcgactt ccagtagatc cacggccagc cataatgggc
     6001 gcccaccggc acgttggtga gataatcggg cacgagatcg gggccaagct ggtcgcgctc
     6061 gttcaccgtc gtccacaatt cctcggtgga ggggttccag gccaggccat tggggttgcg
     6121 caggccagcg gcgaactggc gatgctggcc ggtcttgagg tcgatctcat agatcgccgc
     6181 gcgacccttt tccgcctcca tgccgcgctc gccgatattc gaggcagagc cgatcgccac
     6241 atagagcagc ttgccatcgg ggctgagcag cagattgcgc atccagtggt tgccaccacc
     6301 cggcaaatcc atgattttgc gtggatttcc ggaaatctgc gcgtcgcccg gatggtaatc
     6361 gaaggccagc acggcatcat gatcggcgat gtagagcgtg ttatcctgcc aggccatgcc
     6421 agagggcgat tgcagccctt gcgccttcag cacgatgcgc tggtctgcct ggccggtgcc
     6481 gtggctgtcg cgcagcagca caatcctgtt gggcgaggcc tcgcccgcgc cgacaaagcc
     6541 catcagcagg ccctgcacaa agccggtgat gccgcccggg ccattgcccg gcggcgcgtt
     6601 ggtttcggcc accagcacat cgccattggg cagggtgagc atcgtgcgcg gatgatcgag
     6661 cccttcggca aagcgggtga cgacaaggcc cacggccgcc ttgggcccct cacccgccgc
     6721 ccagcccacc ggctttacca gcgagaggct gggcaggctt tccggcgcgg gatcgaccag
     6781 cttgggccgg gccccctcga acagttccac cgactgatcc gacgggttgc catgggccac
     6841 ccaggcgaag gcacccccgc ccaccagcgc gacagcaccc agagcgatca acgtattgcg
     6901 caaagccgtg ttcatgccag cgggataagc gcttagcccc aagcgggcaa gcgttaaggc
     6961 ccggcttaga tgccgccgcc tgtgaggcgc tggcagatga gatcgagctg atcgaggctg
     7021 cgatagcgga tggtgacggc acccgaacgc ggatcggcat cgacgctgat cttcaccggc
     7081 aggccgagaa tttcttccag ctggttctgc atcatggcga tgtcggcgga ttcggggctg
     7141 tgcttgtcgt cgcgggcggc gcggcgcggt tggggcgctg catcgccgcg ctgctgcttg
     7201 cgcaccagcg cttccacctc gcgcaccgac aattgcttgc ccactgccga ttgcgccagc
     7261 gtttcggcat tgtccacgcc gatcagcgcg cgggcgtggc ccatggagag atcgccggtt
     7321 tccaccagct ggatcaccgg ctcgggcagc atcagcaggc gctggatatt ggcgacatgg
     7381 ctgcgcgact tgtccaccat gcgggcgatt tccgcctgcg tcatgctttc agtctcggcc
     7441 aggcgttgat aggcgcgcgc ctcttcgatg gggttgagat cctcgcgctg gaggttctcg
     7501 atcagcgcca gcgccatcac ttcgcgctcg gtcagatcgc gcaccagcgc gggcacttca
     7561 tgcacatgcg cgcgctgggc ggcccgccag cggcgttcac cggccaccag ctgatagcgg
     7621 ccattgccga aggggcggac gatcaccggc tggatcaccc cgcgcgcggc aatcgaggcg
     7681 gccagctcat caagcgccgc ctcgtcgaaa tggcggcgcg gctggtgggg gtgcggctcg
     7741 atatcggcca cggccagcaa ggccagcccg ccggtcaccc catcgcggcg ggcgggcgcg
     7801 ccggcatctg tgccatggct cgccaccacc ggttcttccc tgcggctttc gcccagcagc
     7861 gcttccagcc cgcggcccag cgccggcccc ttgcgccggc cagtgcccat cgggcccggc
     7921 tggggaatcc tgaaaccggt ggccccctct tcgctcatgc ggctttcctc ttctcgggca
     7981 ggcgggcgat cagctcgcgc gccagcgaca gataggcgcg gctgcccgcg caatgttggt
     8041 cgtagatcag cgccggcagg ccatggctgg gcgcttcgga caggcgcaca ttgcgcggga
     8101 tggccgtttc gaacaccagc tggccgaggc aggagcgcac atcgtcggcc accaaatcgg
     8161 tcagccggtt gcgcttgtcc gacatggtga gcgcaacgcc gatgatgccc agatcggcat
     8221 tgaagcgctg ctgcacccgc tccaccgtct gcagcagctg gctcaaacct tcgagcgcga
     8281 agaactcaca ctgcagcggc accagcagcg tgtcggccgc ggtcagcgcg ttcagcgtca
     8341 gcagacccag cgagggcggg caatcgatga aacagatgtc gtagccggcc gccggcccgc
     8401 ccttggccag ggcatcgcgc agccgctcgg cgcgctcgcc cacacccacc agttccactt
     8461 cggcgcccga cagatccacc gtggccggca ccagatcgag cccctggaac tgggtttcga
     8521 tgatgcaatc ggccagcggt tcatcgccga tcagcaggtc atagctgctg cgttcccgcg
     8581 cttgcggccc cactcccacg cctgtggaag cattgccttg cggatcaaga tcgatcagca
     8641 gcgtgcgcca gccattggcg gccagcgcgg tcgccatgtt gatcgccgtc gtcgttttcc
     8701 ccacgccgcc tttttgattg gcaatggcaa tccggatcat cgcttcactc ccgatttccc
     8761 gaaaacatgg cccgagatga tgccggcttc ggcatcggtt agcgatggtc gcacggtgag
     8821 cgcgtgatgc caaccattta ggccctgcaa ttcctgcgcc gctgaacggc ctttgggcaa
     8881 cagccaagtt gtggcggctg tggaaaaacg tgcggataga gtcagcaaac ggtcaagtgg
     8941 ggcaaaggcc cgcgcagaaa tcacgccaac gtggcgcgtt tgcaccagtt ccagccgctg
     9001 gccgatggtg ctggcgcgct ccagccccag ctctgcgcac aggtgattga gccaggcgat
     9061 tcgccggctg cgcgattcca ccatcaccac ttcggcatcc ggccgcaggg ccgcgatgat
     9121 gattcccggc agaccagcgc ccgtgccaag gtccagccag ggggtggatg tttcacgtgg
     9181 aacaagcgcg agcagctgcg ctgaatcggc gatatggcgc cgccacacat ctgcaaggct
     9241 ggcggcagac accagattct ggcgctggtt ctcctcggcc agcaaggcga tgaaacgctc
     9301 cagccgggca aggccggcgc tatccacatc cggcaggccc gctacaaagg cgcgggcttc
     9361 ctcttcgctt tcgatcatcc ttcagccccc gtggcaatcc gccttgcatg taccagcagc
     9421 gccgccagcg ccgcaggggt gatccccctc acccgcccgg ccgcagccag cgtggcgggg
     9481 cgcgcggctg tcagccgctc caccatctcg cgcgagaggc cggcgatcgc gccataggga
     9541 aaaccatcgc ccagcggcag cgcctcgctg gcgcgcaggt cggccagctc gcgctcctgc
     9601 cgggcgagat agggggcata ggtggcatcc tcggccacct cctccagcac atcggcatcg
     9661 gcataatgag ccgcatcaat ccagggggac agcgccgcca tatccacgcc gccgaagcgc
     9721 aaccattcgc gcaagggcag ccggcccaga tcgctgcgcg ccggcaggcc ccgggcagac
     9781 agttccgccg cgcccacttc gcgttccagc gcctgctcca gcgcggcctt gcgctgctgc
     9841 ctttgggcaa accactgcgc ccgccctgcc cccacgcagc cggccgccat ggccagcggc
     9901 gtcaaccggg tggaggcatt attggcccgc aggcgcaggc gatattcggc ccgcgcggtc
     9961 aacatgcgat agggttcgga cacgccctgc agcgtcagat cgtcgatcat caccgccata
    10021 taggcattgg cccggtcaag cggcgcgggt gcccgctgca gcacggcggc ggtggcatgc
    10081 atgccggcca ccaatccttg cgcggcggct tcctcatagc cggtggtgcc attgatctgc
    10141 ccggcgcaat aaagcccggg gatcgcccgc agctccagac tgggccgcag agcgcgcggg
    10201 tcgatatggt catattccac cgcatagccg ggcaccacca tctccacccg ttccagcccc
    10261 tccatactgc gcagcatggc cagctgcaca tcggccggca ggctggtgga gacgccattg
    10321 ggatagacca gcggtgtatc cagcccttcc ggttccagga agatctggtg gctgtcgcgg
    10381 tcggcaaagc ggtggatctt gtcctcgatg ctggggcaat agcgcgggcc cgccgcgccg
    10441 atcgccccgg tgaacagcgg cgagcgatcc agcccgcccc ggataatctc atgcgtggcc
    10501 gcattggtgc gggtgatcgc gcagaacacc tgcgggttgg cccgctgcgg cccgcccatg
    10561 gcagacatgc tccaaggctc tgcatcggaa ggctgctcct ccagccgggc ccaatcgatg
    10621 gtgcggccat cgaggcgcgg gggcgtgccc gtcttcagcc gcgccatggg cagatcggcc
    10681 gcgcgcaatt gcacggccag ctgctgggca gaagcctcgc caatgcggcc gcccaccatt
    10741 cgctcctcgc cgcggaacag ccggccgccc aaaaaggtgc cggtgcacag caccaccgcc
    10801 cctgccccca gcgccgtgcc atcggccaga tccacaccgg ccacgcgccc gccggccatg
    10861 cgcagcgccg ccacttcgcc ctgcaccagc gccagatcgc cctgctggcc cagcaggtgc
    10921 tgcaccgccg ccttgaagcg cttgcgatcc gcctgcaccc gcggcccctg caccgcgctg
    10981 cccttcgagc gattgagcat gcgataatgg atcgccccgg catccgccgc ccggccgatg
    11041 atcccgtcaa acgcatccac ctcgcgcacg agatggccct tgcccaggcc gccgatcgcc
    11101 ggattgcaac tcatcgcgcc aatggcatcg agatcgaaac tgaccagcgc cgtgcgggca
    11161 cccatgcgcg cggccaccgc cgccgcttcc acgccggcat ggccgccgcc caccacgatg
    11221 atgtcaaact gctgcatgcc tgcccgttag cccagccggg gcttgcggtc aaaacccatg
    11281 ttccacgtgg aacatcactt gccgatgcag aagcgcccga acagcgtatc cagcatatcc
    11341 tcggtagtgg atcggcccag cagccgatcg aacgccaccc gcgccgcacg caaatcttcc
    11401 gccaccagca gcagatcccc ctgcccttgg caacgggcca aggcgagatg cgcttctgcc
    11461 agcagccggt gctgccgcgc attcactgct gcctcacccg gcgccggcat ggcatgtcgc
    11521 gcggccgccg ccagggcgga aaccagacct gccatcccct ccccctgcat ggccgaaaca
    11581 cggtggcgcg gcgcagcctt gcccacatgg ccagcgcggt ccacctgcgc ctcgatctcc
    11641 caggcaccgc ctggcccttc gccctccggc cccagccaca gcaccagatc ggccccctcc
    11701 agcgcggccc tggcccgggc gatgccgatc tcctcgatgg cgtcgcctgc ctcctcgcgc
    11761 aggcccgcca tatcgatgaa ctggaacggc accccctcca gccccacgct gcgcagcaac
    11821 acatcccgcg tagtgccggc gatcggcgat gtgatcgccg cttcgctttc caccagcgcg
    11881 ttgaacagcg tgctcttgcc agcattgggc gggccggcga tcgccacgcg aaaaccttca
    11941 cgcagaacct ctgcccgcgg ccgcgccagc caagcagaga gcgtgtcacc caaggcagaa
    12001 agttccacgt ggaacatttc cggtagcgcg ggcacatcgt cctcatcgcc aaaatcgagc
    12061 aaggcctcca ccacagccga caggcgcaac acgccatccc gccacgcgcc tgcctgccgc
    12121 gacaattgcc cccccgccat cgccaggctg gccc
//
LOCUS       DASRYE010000035        29729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_231553,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000035 DASRYE010000000
VERSION     DASRYE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 29729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29729
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231553"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFF98_01835"
     CDS             complement(<1..395)
                     /locus_tag="VFF98_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62396.1"
                     /translation="MTAPAEHPGAEHHDADLSAWMAVAAGSLGAMMATLDISITNSAL
                     PTIQGAIGASGTEGTWVATSYLVAEIIIIPMSAWLERLFGLRSLLLVAVTLFTLFSMV
                     CGMATTLPQMIIGRVGQGFTGGALIPTAMT"
     gene            complement(392..1549)
                     /locus_tag="VFF98_01840"
     CDS             complement(392..1549)
                     /locus_tag="VFF98_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU62397.1"
                     /translation="MPQSDVPAENASDSAEENGGRIRPGLRKVLLGAGSVLAVAGICG
                     FAYYEIYGRYQQSTEDAYVQADGVTVSPKVAGYVEKVFVVDNQAVKAGDPLVQIDPRD
                     YRAQTAQYAAQIDAAKASATGAEAQIRQQQAAIDKAAADLAVARDQAAYAADQVGRYA
                     PLAATGAETREHLASLKNDAAQARGRADAAAAALASARQAIGSLKAQLAQANAQGEGA
                     QAQLDAANVNLSSTLIRAATDGRVGDKGVRQGQFVQPATRLMTVVPVNQIYITANFKE
                     TQMGLMRPGQPVHVRVDALPGVDILGKVDSVSPGTGAQFSVLPPQNATGNFTKIVQRV
                     PVRVAIYAGPETRQLLVPGMSVEVTVDTRSARDANQRIREEQERHNRAAAQ"
     gene            1636..2064
                     /locus_tag="VFF98_01845"
     CDS             1636..2064
                     /locus_tag="VFF98_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU62398.1"
                     /translation="MKLTDEALITDLGRVYLLMHRRMNRALSAQGLSLARIKTLLCIR
                     RQQGTARAADLADFLDQTPRTVTEMLDGLERDGLITRLPDPDDRRVKRLVLTAQGEQL
                     VTTTEPLRGRIIHEIFTHFAPEERQGLRRALDRFLGELAE"
     gene            2166..4238
                     /locus_tag="VFF98_01850"
     CDS             2166..4238
                     /locus_tag="VFF98_01850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZU62399.1"
                     /translation="MTRLRKGFARFLLLGAAWLPCALPAAEAPTAPAPAPAPAPAHSA
                     ADFAQIPFVENASISPDGLHIAGIFGLDGARRIAILNLFDRSEKPVVVAMPDGTEADD
                     VQWVNRDNIIVRLRMLIPLNLPYVTGPDRVYVTRVVAVNRISHKLTRLLWNMHGLDAA
                     DVVHLPTDGSAEIEIAAQDSIFKEAEFWPTVYRVNVETGQLHMVQAGREDVLEWTADT
                     TGRVRLGMGYEDSTRSFRLLYRGEHADVFHKVDQADTRKRERVIQPLVFLPGGDHALV
                     RHEDEHGHDQIYEMDLISRQDVHPFYTAPEGQWVQHVILSADESTVLGVRLGGGDRAI
                     HWLDPTLAEVQAQFDKAVGNRRAEILSLTPDRKRMLVLLDRADTPGALYFYDIDQGRL
                     QKLAPLNQPLGTTPLAPVRHVSFHARDGLAIEAILTLPPGLPAYKLPIIMLPHGGPWA
                     HDSLDYDYWAQFLAAQGYAVMQPNFRGSTGYGTAFERKGEGQLGLAMQDDITDGLKWA
                     VAQGLADPARACIMGGSYGGYAAMWGAAKDPDLYRCAISIAGVASLRREVNGFGDYLM
                     GNKYRDDWGRMSADFAAVSPANAADRIKAPLLLIHGSKDVTVNPAQSELMASRMRAAG
                     KTFEYLPLPLADHHFGRQPDRQKLLETVAAFLARYNPANSPTIPAAGPANPPPQASAA
                     PAKGAVAQ"
     gene            complement(4232..5611)
                     /locus_tag="VFF98_01855"
     CDS             complement(4232..5611)
                     /locus_tag="VFF98_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU62400.1"
                     /translation="MVCISKRRGSACLSLSVLAGLMGPCGAGSAQAQQVVDAAPAPAA
                     ADSAPQDFALHGQVTVTAQQATGFASPYTGTNSFLPNQLRETVDATLYFGVRPWQGAE
                     LWLNPEVDQGFGVSNTLGVAGFVSGEAYKVGKAAPYVRLQRAFLRQTVQLGGAVQQVD
                     AAANQLRGSTTANRLVFTIGKFSLGDVFDTNRYAHDPRADFFNWSIIETGSFDYAADA
                     WGYTYAAAGEWYQGAWTLRLGLANLSTVPNTTELETNLSQFQLLGEVEHRHTIAGHPG
                     ALRVTVFRNHGRFASLAEASAVAESTGTMPDLATLRRPANRWGGMLNAEQELTDTLGV
                     FLRAGLTDGRYEAYDFTDIDRSLSFGLSLKGTHWGRAADTVGLAGVVNGASKARQRFL
                     ADGGLGILVGDGALAHPGNEWIGEAYYDWQPFSHFNLTADYQLVVNPGYNRDRGPASV
                     LGLRAHLAY"
     gene            complement(5783..7666)
                     /locus_tag="VFF98_01860"
     CDS             complement(5783..7666)
                     /locus_tag="VFF98_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU62401.1"
                     /translation="MRLFSGLSIRQRLIRCLAVLVAVSVVQVSASSFLQWQMRGYATR
                     QKLMADVQRNQMFGDMKHDAIQGDMFRLGDAMQRHDAPARDRAFAGLRQDIDGLTGAY
                     AFVFSQTYPEGLQKRVDDTIGPERDYIERAKAAADRLLTHPEDARGEMDSFTASFDRF
                     EHVQDTLKDALTARMDADSTAADRLMLVALGLMLVTILAGAAALGWAGLFVWREVVEP
                     ITRLTATLQAMAHGDYAEEITGDEAGDEVAQMAAAAAVFRETALAKMMAEMEQEEVVT
                     ALSTGLSKLAAQDLEYRIQIQFPDNYERLRTDFNSAQMALAQAIGSVRVGANSVMKAI
                     TEIRAATDDLSRRNEQQAASLEETAAAMSQVTEGVAETATRAMSVRETIAQAHDEASE
                     GGTVVNRAIEAMAGIERSAQEITSIIDVIDGIAFQTNLLALNAGVEAARAGDAGKGFA
                     VVASEVRALAQRSAAAAQDIKELITTSTGQVDAGVALVGETGALLGKIVARVGEVTGL
                     IGEIAGSAEVQAGSIRQVNGAVGEMDLMTQQNAAMVEETTASTRSLSEEAEQLASLVR
                     TFRTRDVEAREEQAGNVARLRRRRTLASEDMMAPEPQARGGAVAGNLALAPSPADGDW
                     SAF"
     gene            7830..8900
                     /locus_tag="VFF98_01865"
     CDS             7830..8900
                     /locus_tag="VFF98_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62402.1"
                     /translation="MLARLPRPLLLLITLAILAISAWCLTIKPPPIRVAKKGGYTDVH
                     LYKDITEGMRQGQTYYQAAVATQRAHHYPLKPFITVRPPTLVWLAAHLGWRGVDAIAG
                     VLVFIAALCWFGASEIGLSFAERLGITTVVAGASAHAVFSTVLIALHEYLAGICIALA
                     LAGVFAGKHRWGLPLLAAALGLAVRELVLPFVLLALAFALVERRWREAAAWGALVATF
                     GLAMAFHAQAVNALVLPGDITSPGWHAGQGFSAFLKAVIYTSYLAPLPQPVALLMAML
                     PMVGWLALDGRRGAFCCLLVAGYAVMIAFFSRADTFYWGAIMLPWYFIGYVLLPRAVL
                     QLWGVARGKPGSFVIGKKPASL"
     gene            8944..10623
                     /gene="ettA"
                     /locus_tag="VFF98_01870"
     CDS             8944..10623
                     /gene="ettA"
                     /locus_tag="VFF98_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZU62403.1"
                     /translation="MAAQYAFVMKSMTKTFPGAQKPVLNNISLQFYQGAKIGIVGPNG
                     AGKSTLMKIMGGIDTDYTGEAWAGENITVGYLPQEPQLDPTKSVLENVKDGARETADL
                     VDRFNEISNIMADPPEDADFDALMEEMGELQGKIDAVDGWTLDNQLEIAMEALRCPPS
                     DWPVESLSGGEKRRIALTRLLIQKPDILLLDEPTNHLDAESVEWLENHLKEYHGAVLM
                     ITHDRYFLDNVVGWILELDRGKYFPYEGNYSTYLEKKSQRLAQEDREATGRQKAINDE
                     LEWIRAGTKGRQTKSKARIKKFEELVASQENRGPGKAQIVIQVPERLGGKVIEAKNIS
                     KAFGDKVLFNDLSFLLPPGGIVGVIGPNGAGKSTLFKMITGQEKPDSGTIEIGSTVRL
                     GYVDQSRDHLDPTKNVWEEISDGLDYMKVNGFDTSTRAYVGAFNFKGQDQQKNVGKLS
                     GGERNRVNIAKMLKRGGNVLLLDEPTNDLDVETLAALEEAIENFAGCAVVISHDRFFL
                     DRLATHILAFEGNGHVEWFEGNFEAYEEDKRRRLGDAADRASAGAYKKLTR"
     gene            complement(10802..11218)
                     /locus_tag="VFF98_01875"
     CDS             complement(10802..11218)
                     /locus_tag="VFF98_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62404.1"
                     /translation="MRHHHLAATAIAAAHHGLAATLRLATALRPALRLAAALGLATAA
                     MIAATALGLATAMVAAAALGLAATMVAAATLGLAAALTTALAATMFVARAGLGLCHRR
                     GRGHQHGHHESGAGALAQRRRNPGHHRTSYPAGAEM"
     gene            11204..11725
                     /locus_tag="VFF98_01880"
     CDS             11204..11725
                     /locus_tag="VFF98_01880"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZU62405.1"
                     /translation="MVMPHGVSGQPMMNGGNRPGGQPMMNGGNRPGGQPMMNGGNHPG
                     GQPMMNGGNRGPDGRGGPDGRGGPDGRGWSRDWRNDSRYDWRGWREGNRDAFHIGRYY
                     PPYRGYGYRRLGVGFMLDPLFFASSYWIYDPWFYHLPDAYGPYHWVRYYNDALLVDTS
                     TGQVVEVLNDFFW"
     gene            11846..12529
                     /locus_tag="VFF98_01885"
     CDS             11846..12529
                     /locus_tag="VFF98_01885"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZU62406.1"
                     /translation="MFTALRPARPAADRSALERLGSAVRQRLDADPAAWRIPVEAAEI
                     YAIGQFLSAQECTRFIAMVDEVAQPSATFDPSSQQTYRTSFSGDVDREDPFVRMIERR
                     IDDLLGIAPAYGETVQGQRYRPGQEFKAHHDWFYTDAAYWPQEKRHGGQRSWTAMIYL
                     NQVEAGGATVFPDLGVSVTPEPGMLLAWNNATPDGLPNPATLHAAEPVASGVKYVITK
                     WYRTRRWGA"
     gene            complement(12567..14192)
                     /locus_tag="VFF98_01890"
     CDS             complement(12567..14192)
                     /locus_tag="VFF98_01890"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZU62407.1"
                     /translation="MTDNELIAAAQLSKAWPFEEARKLVKRYPQGKRDGDGNLVPVLF
                     ETGYGPSGLPHIGTFQEVLRTTLVRRAYEGLTGGHPTRLVAFSDDMDGLRKVPDNVPN
                     GDLLAANLGKPLSRIPDPFATHDSFAAHNNAMLRAFLDRFGFDYEFVSAADRYNAGDF
                     DEALRGVLRAYDKIMGIMLPTLREERRKTYSPILPVSPTSGVVLQVPVEVVDAEAGLV
                     RFEDASVPGGQIIEHCIFQGGAKLQWKVDWAMRWVALGVDYEMCGKDLTDSVTQSGKI
                     AQALGGRKPEGLIYELFLDEKGEKISKSKGNGLTIEQWLTYGSEESLGFYLFREPKSA
                     KSLHPGIIARAVDEYWQFREKLAVQPLEQQLGNPVWHLLRTNGAGEGAGEGERLPVTY
                     GLLLNLVGVLGASATREQVWSYLANYVEDARAEAHPALDTLVTCALAYNRDFIAPTLQ
                     RRKPEANEAAALAALDEELAATSDDASAEELQNIVYEIGKDPHYGFESLRDWFKALYE
                     TLLGSAQGPRMGSFIALYGIGNTRALIAEALDK"
     gene            14345..17158
                     /locus_tag="VFF98_01895"
     CDS             14345..17158
                     /locus_tag="VFF98_01895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZU62408.1"
                     /translation="MPAPLSLPALHASHSGCWLREKGGPTRPCAKGEAIVAAADTPLL
                     LLNAPLVASRLGYPDLSGLDLLELYAFIHPARFIVPTARGLAQALGIGAAEGGAGSTE
                     TEITDADVPALLQQAAACLIATCAAPDWPEREGAWTALQALTRLRWPWAALLAPHIAQ
                     PDRAERWLFTRLPEWEEAAERPQPAQVTLDESDVLARLAALTGEGAEHRPGQQAYAAA
                     AARIFAPRGHPKAPHMVLAEAGTGTGKTLGYLAPSSLWAQAAGGTVWVSTYTKALQRQ
                     LRRESRRVWADSQPGPAPVVVRKGRENYLCLLNLEDALQGGFTGRPAVLAQLVARWAA
                     YSRDGDMIGGDLPGWLGTLFRQRGITALTDRRGECVYAGCPHYRKCFIERAARASAEA
                     QLVIANHALVMVNAARGKDVAQRPTRIVFDEGHHVFEAADSTFAADLTGAETIELRRW
                     VIGPEKGSRGRRRGLAARLSDIASYDEEGGRAIAAAREAAEALPGDGWLGRVVEGEPS
                     GPLEALLAAVRALVYARDESGGQEAGYALETEAANLDGDVVEAAQTALAALGQLRRPL
                     IQLGLRLEAVIADGPDWLDGQGRARIEGARQSLTWRVELIGAWEALLGGLGGAADPAF
                     VDWLAVDRSDGREFDIGIHRRWLDPMKPFAASVLAPAHGVMLTSATLRDGKHAGAEEG
                     WEAAIARSGAGWLEGPAHLASFPSPFDYAAQAQVLIVTDVPKGDIPALAAAYARLVEA
                     AGGGVLGLFTAIRRLRAVYGRIADRLARAGLPLYAQHVDPIDTGTLVDIFREDPHASL
                     LGTDALRDGVDVPGHSLRLVVMEQVPWPKPSILHRARRLADADGGQAHDDRIIRARLA
                     QAFGRLIRSRGDRGHFVVLSAAFPSRLLSAFPQGTPVLRCTLDEALQRIGGGVSRPQE
                     HAADETDSPPAPHNMPFPGWLPD"
     gene            17158..17727
                     /locus_tag="VFF98_01900"
     CDS             17158..17727
                     /locus_tag="VFF98_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZU62409.1"
                     /translation="MKKLGLFRHAKSDWSDQRARDFDRPLNARGRLGAPLIGRHIRDY
                     AEAEQFRWDRVLASPAVRSAETIELAAAGAGMEAARSFAINWDRRIYLASSAALMDVL
                     REQSDALDAILMVGHNPGLEDLIFDLVPDDGSSPLRDVVEAKFPTAAFAVLELAIAHW
                     QDVEEGCARLVHLMRPRDLDPALGPELHD"
     gene            complement(17763..18209)
                     /locus_tag="VFF98_01905"
     CDS             complement(17763..18209)
                     /locus_tag="VFF98_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZU62410.1"
                     /translation="MVPAAAPALSGAASGSAAGAALPADAKAPDALRNIGEVARLLGV
                     KTHVLRYWESQFPALRPVKLAGGRRYYRPADVALLQQIHTMVHGQGFTLAGARRQLRA
                     AATEGAVEQPLPAAEAPPPGADAGAQAALLAGLRLLRDRLARALDG"
     gene            complement(18257..18592)
                     /locus_tag="VFF98_01910"
     CDS             complement(18257..18592)
                     /locus_tag="VFF98_01910"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HZU62411.1"
                     /translation="MREGRHSRQRGARMRSINTLTRADLAEAINRKMGLSRTESLTMV
                     EAILQHMSDALAAGENVKISGFGTFLLRDKTERVGRNPKTGVEVPITPRRVLTFRASQ
                     MLKQRVSGS"
     gene            complement(18714..19685)
                     /locus_tag="VFF98_01915"
     CDS             complement(18714..19685)
                     /locus_tag="VFF98_01915"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZU62412.1"
                     /translation="MIRRSVLIGTGSALPSRLVSNAEMAELVDTTDEWIVERTGIRQR
                     YVAGEGESTSTLAIAAARAALDAAGIEGASLDLIVLATATPDQTFPATATIVQQALGA
                     NGGIAFDVAAVCSGFLYALGVADSMLRTGMATRALVIGAETFSKILDWEDRTTCVLFG
                     DGAGAIVLEARESEDASAPGVLATKLHADGAHNQLLFVDGGPSTTGTVGKLRMKGREV
                     FRHAVVNLSSVLGEVLDEAGFTPADIDWLVPHQANARILEATARKLGMPTEKVVMTVA
                     EHANTSAASVPLALDRALRDGRIQPGDLVMLEAMGGGFTWGASLIRI"
     gene            complement(19682..20761)
                     /gene="plsX"
                     /locus_tag="VFF98_01920"
     CDS             complement(19682..20761)
                     /gene="plsX"
                     /locus_tag="VFF98_01920"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZU62413.1"
                     /translation="MSVPRIAVDAMGGDDGVRTMVAGVALAVERGLACGLLLVGDRPR
                     IAAEVARHPRLQTAQAQGLLDILHADEVISGEEKPSQALRRARVTSMGLAVDAVKSGK
                     AGAALSGGNTGALMAIAKLALRTMPGIDRPALAALMPTLADSDLVMLDLGANTECDAR
                     NLVQFAIMGAAYSRIVIDSAAPRVRLLNIGTEDIKGTEQLRDAAAVLRGATDLPLRFD
                     GFTEADKLCRGEVDVVVTDGFSGNVALKAMEGTARFVGDLLRRSFASSLRSKLGFLIS
                     KPATELLKHHLDPNNHNGAVFLGLNGVVVKSHGSANAKGVANAIGVTARLLQENITQR
                     IAADLEQLGAENLRMAHKVTLEAHQ"
     gene            complement(20790..20969)
                     /gene="rpmF"
                     /locus_tag="VFF98_01925"
     CDS             complement(20790..20969)
                     /gene="rpmF"
                     /locus_tag="VFF98_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZU62414.1"
                     /translation="MAVPKRKTSPSRRGMRRSHDALKVEAFHECSNCGELKRPHNLCP
                     SCGHYNGREVLAVGL"
     gene            21153..21557
                     /locus_tag="VFF98_01930"
     CDS             21153..21557
                     /locus_tag="VFF98_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZU62415.1"
                     /translation="MLLPTTLCLAAAAAVVNFWLGLRIMRLRMARKIFMGDGADPAIV
                     ARMRAQANFIENTPITLILFGAVEALGGAHLFWGGAWLAPLGAIFMLGRVAHAFGMDG
                     RFKPGRPIGMLTTLFTQIVLAVVAVLASTGRL"
     gene            complement(21592..22275)
                     /locus_tag="VFF98_01935"
     CDS             complement(21592..22275)
                     /locus_tag="VFF98_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU62416.1"
                     /translation="MAASTDPQTDAAQPPLRAVIIPVTAFQQNCTLLWCTKTMKGALV
                     DPGGDLDKLKAAVAQYGVTLEKLLVTHGHMDHCGLAGVLADELALPLEGPEEGDRFWI
                     EGLDNPNTRMGLEGKSFEPTRWLHDGDTVTVGDLVFDVVHCPGHTPGHVVFVHAPSRL
                     ALVGDVLFAGSIGRTDFPKGNQQQLIDSITEKLWPLGKDITFIPGHGPCSTFGHEMLT
                     NPFVGSRYG"
     gene            22401..22913
                     /locus_tag="VFF98_01940"
     CDS             22401..22913
                     /locus_tag="VFF98_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZU62417.1"
                     /translation="MRAPALFLALAALALPQTASAAAAPHLAEQGFGDLARPLPLPYD
                     GAADAHAAIAAARARAAAGGKLLLIDLGGNWCPDCRILAGTLALPDLALFVRAHFEVV
                     TVDVGYFTKNLDIAESYGLGRRPAGVPAVLIVEPKSNRLVNKGHEVALADARTMRPQA
                     IADWLAGWVR"
     gene            complement(22929..23561)
                     /locus_tag="VFF98_01945"
     CDS             complement(22929..23561)
                     /locus_tag="VFF98_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HZU62418.1"
                     /translation="MPIADPRNRLLALADARGVSLAALSALIGRNASYLQQFVRKGSP
                     RKLDEADRRKLARFFGVDEAELGGGQEISHGPGRWVDVARLALDASAGPGAVPQGEAA
                     VGILRFSAAWLRAQGLDPAQVAAMAVAGDSMEPTLRDGDEILVDRRRRPLRDGVYVLR
                     RGDSLLVKRVAASGAGRLLLISDNPAYPPIDLPAGEVVPIGRVVWKGGRL"
     gene            23660..23863
                     /locus_tag="VFF98_01950"
     CDS             23660..23863
                     /locus_tag="VFF98_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62419.1"
                     /translation="MLLHKIEQFLRQSGMPWTKFGRIVAHDPRLVGDMRNGRNPREAM
                     CARIEAFIAAGEAPAAEETPHAF"
     gene            23853..24281
                     /locus_tag="VFF98_01955"
     CDS             23853..24281
                     /locus_tag="VFF98_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62420.1"
                     /translation="MRFDRKPAGQHRADLLAPFSAARPASTLPAAGPQARPRLRHPSL
                     QVLEAMLRLAGPQAQLLSHAERPWASATFSGTRHTITLLFDGAGAVAAAEALIAALPE
                     HEFTIRGQLVADATVSEVLHTLLPAERMVVEADLLLLAEG"
     gene            complement(24328..25794)
                     /gene="cysS"
                     /locus_tag="VFF98_01960"
     CDS             complement(24328..25794)
                     /gene="cysS"
                     /locus_tag="VFF98_01960"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZU62421.1"
                     /translation="MSDAIPLQAPLRLFNSLTRQLEDFAPAHAGEARVYTCGPTVYNY
                     AHIGNMRAYVFADVLGRTLNHKGYRLTHVINITDVGHLTDDADAGEDKLERQAAREAR
                     SIWDIAAHYTAAFKADIDALNIRQPAQWSVATDYVPRMIAFAEKIAARHCYELPTGLY
                     FDVSTVADYGRLARAATEDGEGRIEKVDGKRNAADFAIWRKTPPGETRQMEWDSPWGR
                     GAPGWHLECSVMSGELLGFPFDIHTGGIDHREIHHPNEIAQNQAFCCTGGLDVPANSG
                     AKIWMHNNFLVERSGKMSKSSGEFLRLQLLIDRGYHPLAYRLMCLQAHYRSELEFSWE
                     GLSAALTRLKRLLMAVAQVRAQGAEHAAPGPKFAPLLERFEAAIGDDLATPTALTVLE
                     EAAALKKVPAGEKLAAIAAMDGVLGLGLLSLTRADLRLRPKVASIAETEIEAILAQRQ
                     QARAEKDFARSDALRDALAAQGVDAMDGDPLGWEWKLA"
     gene            complement(25862..26536)
                     /locus_tag="VFF98_01965"
     CDS             complement(25862..26536)
                     /locus_tag="VFF98_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZU62422.1"
                     /translation="MSALSVSEAVATRRSIRAFLDQPVDEAILRRVLEKARMAPSSTN
                     LQPWRGVILAGRPLADLSAALLAADPASQPEYPLSAPETPAHFIARREAIMAERMDAL
                     GIAREDKEGRAAYNRKNYEFFGAPACLFTFVPRVLSPAHWASTALWLQTVMLLLVEEG
                     LGSCPQESLAHFPLPIKQVCGIDDGEYFFWCGLAIGWPDPAAPVNRYARPRVPLEESV
                     SFRGFA"
     gene            complement(26607..28421)
                     /gene="cobT"
                     /locus_tag="VFF98_01970"
     CDS             complement(26607..28421)
                     /gene="cobT"
                     /locus_tag="VFF98_01970"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HZU62423.1"
                     /translation="MAEDTPLDRFKHVLTGAARALAHEPEVEVNWTGDAPVQAGTQFR
                     VPSPGIRLPRAAANEARGAADSFALKLRHHNPALHGRHMPAEPTARACFDAVEQVRYE
                     ALGARAMAGVAANLEAAVERRVAGDPITRAERAGDVPMASALALLLREHLTGQPIPES
                     AAAGVALVRPWIEGRAGGDFARLAENLESQKAFQALALDMLQHLELTEAAPTDDQPEE
                     EGEDDSEDDNEDEQPGDDQQDSPQPMDMAADRQEGGEDGEDEAESEQDDDAEDAEPGE
                     DAEDGMLPTRPNRPWTDDPAGFDYQVFTESFDEVVAAHDLCDEAELKRLRAYLDAQLK
                     GLQGIVTKLANRLQRRLMAQQNRSWDFDQEEGLLDAARLARVVIAPGQSLSYKVERDI
                     EFKDTIVTLLIDNSGSMRGRPISIAAISADVLARTLERCGVKTEILGFTTRAWKGGQS
                     REAWLAAGKPVHPGRLNDLRHIVYKQADEPWRRAKQSLGLMMREGLLKENIDGEALLW
                     AHNRLLARPEDRRILMVISDGAPVDDSTLSVNSAGYLEAHLRKVIEWIEKQSPVQLVA
                     IGIGHDVTRYYRRAVTIMDVEQLGGTMIEQLAGLFEDG"
     gene            complement(28451..29101)
                     /gene="fsa"
                     /locus_tag="VFF98_01975"
     CDS             complement(28451..29101)
                     /gene="fsa"
                     /locus_tag="VFF98_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HZU62424.1"
                     /translation="MKFFVDTAEIADIKELAATGLLDGVTTNPSLIAKSGRDFLEVTK
                     EICALVDGPVSAEVISLDHEGMMREAEILRKIADNVCIKVPLTIDGLKTCKALTSDGT
                     KVNVTLCFSANQALLAAKAGASFISPFIGRHDDNGFDGLDLIRDIRLIYDNYAYETEI
                     LAASIRHGVHVLECAKIGADVMTAPPAVIKGLFKHVLTDKGIEGFLADWAKTGQKL"
     gene            29319..>29729
                     /locus_tag="VFF98_01980"
     CDS             29319..>29729
                     /locus_tag="VFF98_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62425.1"
                     /translation="MASPAPSASLFPDAQPDAPARVRVLVLNAALGALDYKVPPAMQV
                     APGSVVVAPLGPRQILGVVWEAERLEAGEVPAHKLRALLEVLPVPPLAAPLRRLIEWT
                     ADYYCAPLASVVRLALSSSAALRGGRSMREYRLAE"
BASE COUNT         4707 a        10117 c         9989 g         4916 t
ORIGIN      
        1 gtcatcgcgg tggggatcag cgcgccgccg gtaaagccct gccccacccg gccgatgatc
       61 atctgcggca gggtggtggc catgccgcac accatcgaga acagggtgaa cagcgtgacc
      121 gccaccagca gcaggctgcg caggccaaac agccgctcca gccaggccga catcgggatg
      181 atgatgatct ctgccaccag atagctggtg gccacccagg tgccctcggt gccgctggcg
      241 ccgatcgcgc cctgaatggt gggcagcgcc gaattggtga tcgagatgtc gagcgtggcc
      301 atcatcgcgc ccaggctgcc ggcggccacg gccatccagg cggacaggtc cgcatcatgg
      361 tgctcggcgc cgggatgttc ggcaggcgcc gtcattgggc ggcggcccgg ttgtgccgct
      421 cctgctcctc gcggatgcgc tggttggcat cgcgggcgct gcgcgtgtcc accgtcactt
      481 ccacagacat gcccggcacc agcagctggc gcgtttccgg cccggcatag atcgccacgc
      541 gcaccggcac gcgctgcacg atcttggtga aattgccggt ggcattctgc ggcggcagca
      601 ccgagaactg cgcccccgtg cccggcgaga cactgtctac cttgccgagg atatcaacgc
      661 ccggcagcgc atccacccgc acatgcaccg gctggccggg gcgcatcagc cccatctgcg
      721 tttccttgaa attggcggtg atgtagatct ggttcaccgg caccacggtc atcagccggg
      781 tggcgggctg cacaaactgg ccctggcgca cgcccttgtc gcccacgcgg ccatcggtgg
      841 cggcgcggat cagcgtggag gaaaggttca cattggcggc atcgagctgg gcctgggcgc
      901 cctcgccctg cgcattggcc tgggccagct gggccttgag gctgccgatc gcctggcggg
      961 cagaggccag ggcggccgcg gccgcatcgg cgcggccgcg ggcctgggcg gcatcgttct
     1021 tcaggctggc cagatgctcg cgcgtttcgg cgccggtggc ggccagcggg gcatagcggc
     1081 ccacctgatc ggcggcatag gcggcctgat cgcgcgccac ggcgagatcg gcggcggcct
     1141 tgtcgatcgc ggcctgctgc tggcggatct gcgcctcggc gccggtggcg ctggccttgg
     1201 cggcatcgat ctgggcggca tattgcgcgg tctgcgcgcg atagtcgcgc gggtcgatct
     1261 gcaccagcgg gtcgcccgcc ttcaccgcct ggttatcgac gacgaagacc ttctccacat
     1321 agccggccac cttgggcgac acggtaacgc catcggcctg cacataggca tcctcggtgg
     1381 attgctgata gcggccatag atctcgtaat aggcaaagcc acagatgccc gccaccgcca
     1441 gcacgctgcc cgcccccagc agcaccttgc gcagccccgg acggatgcga ccgccattct
     1501 cctcggcaga atcagacgca ttttcggcag gaacatcgga ttggggcacg cagcgaaact
     1561 cacgcttgat cggtcgatgc ccggtcaaat gaacgcagtt gcggaattat gcaagaccat
     1621 ttattaagcc cacttatgaa attgacggac gaagcgctga tcaccgatct gggccgggtg
     1681 tacctgctga tgcaccggcg gatgaaccgc gcgctgagcg cgcagggcct ctcgctggcg
     1741 cggatcaaga cgctgctgtg catccgccgg cagcagggca cggcccgcgc ggcagacctc
     1801 gccgattttc tcgaccagac cccgcgcacc gtgaccgaga tgctcgacgg gctggagcgc
     1861 gacgggctga tcacccgcct gcccgacccg gatgaccgcc gcgtgaaacg cctggtgctg
     1921 accgcgcagg gcgaacagct ggtaaccacc acagaacccc tgcgcggccg catcatccat
     1981 gaaatattca cgcatttcgc gccggaagaa cgccagggcc tgcgccgcgc gctcgaccgt
     2041 tttctgggtg aattggccga ataatggatc tttttattct ttttccgtgt attgcgcagg
     2101 tcacaatgca gtcataaacc ttcctctgca tccggcggtg cgacgccgaa tacaacgggg
     2161 ggtttatgac aagactgcgc aagggctttg cccgttttct gctgctgggt gcggcatggc
     2221 tgccctgcgc cctgccggcg gcagaagcgc ccacggcgcc agcccctgct ccggcccccg
     2281 ctccggccca cagcgcagct gatttcgcgc agatcccctt cgttgaaaac gccagcatct
     2341 cgcccgatgg gctgcatatc gccggcatct tcggcctcga tggcgcccgc cgcatcgcca
     2401 tcctcaacct gttcgacagg agcgagaagc cagtggtggt ggccatgccc gatggcaccg
     2461 aggccgatga cgtgcagtgg gtgaaccgcg ataatatcat cgtgcgcctg cgcatgctga
     2521 tcccgctcaa cctgccctat gtgacagggc cggaccgcgt ctatgtaacg cgggtggtgg
     2581 cggtgaaccg gatcagccac aagctcaccc gcctgttgtg gaacatgcac gggctggatg
     2641 cggccgatgt ggttcacctg cccaccgatg gcagcgccga aatcgagatc gccgcgcagg
     2701 attcgatctt caaggaagcg gaattctggc ccaccgtcta ccgcgtgaat gtggaaacgg
     2761 gccagctgca catggtgcag gccggccgcg aggatgtgct ggaatggacc gcggatacca
     2821 ccggccgggt gcggctgggg atgggctatg aagacagcac ccgcagcttc cgcctgctct
     2881 atcgcggcga gcatgccgac gttttccaca aggtggatca ggccgatacc cgcaagcgcg
     2941 agcgtgtgat ccagccgctg gtgtttctgc ccggcggcga ccatgcgctg gtgcgccacg
     3001 aggacgaaca cggccacgac cagatctacg agatggatct gatcagccgg caggatgtgc
     3061 accccttcta caccgcgccc gagggccagt gggtgcaaca tgtcatcctc tcggccgatg
     3121 agagcaccgt tctgggcgtg cgtctgggcg gcggcgaccg ggcgatacac tggctggacc
     3181 cgacgctggc cgaggtgcag gcgcagttcg acaaggcggt gggcaaccgg cgcgccgaaa
     3241 ttctcagcct cacccccgat cgcaagcgca tgctggtgct gctcgaccgg gccgacacgc
     3301 ccggcgcgct ctatttctac gatatcgacc aggggcggct gcagaagctg gccccgctga
     3361 accagccgct gggcaccacg ccgctggcgc cggtgcgcca tgtgtccttc catgcgcgcg
     3421 acgggctggc gatcgaggcg atcctcaccc tgccccccgg cctgccggcc tacaagctgc
     3481 cgatcatcat gctgccccat ggcggcccct gggcgcatga cagcctggat tacgattatt
     3541 gggcgcagtt cctggcggcg cagggctatg cggtgatgca gcccaacttc cgcggctcca
     3601 ccggctatgg cacggctttc gagcgcaagg gcgagggcca gctgggcctg gccatgcagg
     3661 acgacattac cgacgggctg aaatgggccg tcgcgcaggg gctggccgat ccggcgcgcg
     3721 cctgcatcat gggcggctcc tatggcggct atgcggcgat gtggggggcg gccaaggatc
     3781 ctgatctcta tcgctgcgcc atctcgattg ccggcgttgc cagcctgcgc cgcgaggtga
     3841 acggctttgg cgattatctc atgggcaaca aataccgcga cgactggggc cggatgagcg
     3901 cggatttcgc cgctgtctcc ccggccaatg cggcagaccg gatcaaggcg ccgctgctgc
     3961 tgatccatgg cagcaaggac gtgacggtga accccgccca atccgagctg atggccagcc
     4021 ggatgcgcgc ggccggcaaa accttcgaat atctgcccct gccgctggcc gaccaccact
     4081 ttggccgcca gccagaccgg cagaagctgc tggaaaccgt ggcggcgttt ctcgcccgct
     4141 ataaccccgc caattcgccc accattccgg ccgccgggcc cgccaatccg cccccgcagg
     4201 ccagcgcggc cccggcaaaa ggcgccgtag ctcaataggc cagatgcgcc cgcagcccca
     4261 gcacgctggc agggccccgg tcacggttgt agccggggtt caccaccagc tggtaatcgg
     4321 cggtgaggtt gaaatggctg aagggctgcc agtcgtaata ggcctcgccg atccattcat
     4381 tgccggggtg ggccagcgcg ccatcgccca ccagaatgcc caggccccca tcggccagga
     4441 agcgctggcg cgcctttgag gcgccgttga caacgccggc cagccccacc gtatcggcag
     4501 cgcggcccca atgcgtgcct ttcagcgaca ggccgaacga caggctgcgg tcgatatcgg
     4561 tgaaatcata ggcttcgtag cgcccgtcgg tcagccccgc gcgcaggaac acgcccagcg
     4621 tgtcggtcag ctcctgctcg gcgttcagca tgccgcccca gcggttggcc ggccggcgca
     4681 gggtggccag atcgggcatg gtgccggtgc tttcggccac ggcgctcgcc tcggccaacg
     4741 aggcaaagcg gccgtggttg cgaaacaccg tgacccgcag cgcgccgggg tggccggcga
     4801 tggtgtggcg gtgctccacc tcgcccagca gctggaactg gctgaggttc gtctccagct
     4861 cggtggtgtt gggcaccgtc gagaggttgg ccaggcccag ccgcagcgtc cacgcgccct
     4921 gataccattc gcccgccgcg gcataggtat agccccaggc atcggcggca taatcgaaac
     4981 tgcccgtctc gatgatcgac cagttgaaga aatcggcccg cggatcatgg gcatagcggt
     5041 tggtgtcgaa cacatcgccc agcgagaact tgccgatggt gaagaccagc cggttggccg
     5101 tggtgctgcc gcgcaactgg ttggcggcgg catccacctg ctgcaccgcg ccgcccagct
     5161 gcaccgtctg gcgcagaaag gcgcgctgca gccgcacata gggcgccgcc ttgcccacct
     5221 tgtaggcctc gccgctgaca aagccggcca cgcccagcgt gttcgacaca ccaaagccct
     5281 gatcgacctc ggggttcagc cacagctccg ccccctgcca gggccgcacg ccgaaataga
     5341 gcgtggcatc caccgtctcg cgcagctggt tgggcaggaa gctgttggtg ccggtgtagg
     5401 gcgaggcaaa gccggtggcc tgctgggcgg tgaccgtgac ctggccgtga agggcgaaat
     5461 cctgcggggc gctatcggcg gccgcgggcg caggggctgc atcaaccacc tgctgggcct
     5521 gtgcgctgcc ggccccgcaa ggccccatca gcccggccag aacggacagc gagaggcagg
     5581 cgctgcccct gcgcttcgag atacaaacca tgcaaacccc actcaacggc cgcgcgaagg
     5641 cagccctttc tcctgcgcgg gatcgccgcc cgcgtgattc tgaaccgcgc cgcctatgcc
     5701 gcaatttgcg gccgggcgcg cctgcttatc cgcgggctgc tgatagtttt attacacgcc
     5761 ctgcaaaggc ctgcgcgctg gctcagaacg ccgaccagtc gccatcggcc ggcgatggcg
     5821 ccagcgccag attgccggcc accgcgccgc cccgggcctg tggctctggc gccatcatgt
     5881 cctcgctggc cagcgtgcgg cggcggcgca gccgggcaac attgcccgcc tgttcctcgc
     5941 gcgcttccac atcgcgggtg cggaaggtgc gcaccaggct ggccagttgc tcggcctctt
     6001 cggaaaggct gcgggtggag gcggtggttt cttccaccat ggccgcgttc tgctgggtca
     6061 tcaggtccat ctcgcccaca gcgccgttca cctgccggat cgagccggcc tgcacttcgg
     6121 cgctgccggc gatctcgccg ataaggccgg tcacctcgcc cacccgcgcc acgatcttgc
     6181 ccagcagcgc gccggtttcg cccaccagcg ccacgccggc atccacctgg ccggtggagg
     6241 tggtgatcag ttccttgata tcctgggcag ccgcggcgct gcgctgggcc agggcgcgca
     6301 cctcgctggc caccacggca aagcccttgc cagcgtcgcc cgcgcgcgcg gcttccacgc
     6361 cggcattcag cgccagcagg ttggtctgga aggcgatgcc atcgatcaca tcgatgatgc
     6421 tggtgatttc ctgggcagag cgttcaatgc cggccatcgc ctcgatcgcc cggttgacca
     6481 cggtgccgcc ctcgctggcc tcgtcatggg cctgggcgat cgtctcgcgc acggacatcg
     6541 cccgcgtggc ggtttcggcc acgccttctg tcacctggct catggcggcg gcggtctcct
     6601 ccaggctggc ggcctgctgt tcgttgcggc gcgacaggtc gtcggtggcc gcgcggatct
     6661 cggtgatcgc cttcatcacg ctgttggcgc ccacccgcac cgagccgatg gcctgcgcca
     6721 gcgccatctg cgccgagttg aaatcggtgc gcagccgctc gtaattgtcg gggaactgga
     6781 tctggatgcg atattccaga tcctgggcgg ccagcttgga aaggccggtg ctgagcgcgg
     6841 tgacgacctc ttcctgctcc atctcggcca tcatcttggc cagcgccgtc tcgcggaaga
     6901 cagcggcggc agcggccatc tgggccactt catcgcccgc ttcgtcgccg gtgatctcct
     6961 cggcataatc gccatgggcc atggcctgca gggtggccgt cagccgggtg atcggctcca
     7021 ccacctcgcg ccagacgaac agccccgccc agcccagcgc cgccgcgcct gccaagatcg
     7081 tcaccagcat cagccccagc gccaccagca tcagccggtc ggcagcggtg ctgtcggcat
     7141 ccatgcgggc ggtcagcgcg tctttcagcg tgtcctgcac atgttcgaaa cggtcgaagg
     7201 aggcggtgaa gctgtccatc tcgccgcgcg catcctccgg gtgggtcagc agccggtcgg
     7261 cggcggcctt ggcccgctcg atatagtcgc gctcggggcc gatggtgtcg tccacccgct
     7321 tttgcagccc ttcgggatag gtctggctga agacgaaggc ataggcgccc gtcagcccgt
     7381 cgatatcctg gcgcaggccg gcaaaggccc tgtcgcgcgc gggggcatca tggcgctgca
     7441 tggcatcgcc cagacggaac atgtcgccct ggatggcatc atgcttcatg tcgccgaaca
     7501 tctggttgcg ctgcacatcg gccatcagct tctggcgcgt ggcatagccg cgcatctgcc
     7561 attgcaggaa ggaggacgcg ctgacctgca ccacgctgac cgccaccagc accgcaaggc
     7621 accggatcag ccgctggcga atgctcaatc ccgaaaacag ccgcacatgc aactcccgat
     7681 accaggcccc tggcgggcca catcaccccg atcggccccg ccctagccgg ccgggcgcaa
     7741 aagccgcgtt acccgtgacc ctgtgaaagc ccctagaggc cctgccgaaa gcccttgcct
     7801 tgcggcccac atcgcgccaa ggcggggatg tgcttgctcg cctgccccgc ccgctgctgc
     7861 tgctgatcac gctggcgatc ctcgccatct cggcctggtg cctcaccatc aagccgccgc
     7921 cgatccgcgt ggccaagaag ggcggctaca ccgatgtcca cctctacaag gacatcaccg
     7981 agggcatgcg gcaggggcaa acctattacc aggcggccgt ggccacccag cgcgcccatc
     8041 actatccgct caagcccttc atcaccgtgc gcccgcccac gctggtgtgg ctggccgcgc
     8101 atctgggctg gcggggggtg gatgcgattg ccggcgtgct ggtgttcatc gccgccctgt
     8161 gctggttcgg cgcctcggaa atcggcctca gctttgccga aaggctgggg atcaccacgg
     8221 tggtggcggg cgcatcggcc catgcggtgt tttccaccgt gctgatcgcg ctgcatgaat
     8281 atctcgccgg catctgcatc gcgctggcgc tggcgggcgt gtttgccggc aaacaccgct
     8341 ggggcctgcc gctgctggcc gccgcccttg gccttgccgt gcgcgagctg gtgctgccct
     8401 tcgtgctctt ggcgctggcc tttgcgctgg tggaaaggcg ctggcgcgag gcggcggcgt
     8461 ggggggcgct ggtggcgacc tttggcctgg ccatggcctt tcacgcgcaa gcggtgaacg
     8521 cgctggtgct gccgggcgac atcacctcgc cgggctggca tgcgggccag ggtttttccg
     8581 ctttcctgaa ggcggtgatc tacacctcct atctggcgcc cctgccccag ccggtggcgc
     8641 tgttgatggc gatgctgccg atggtgggct ggctggcgct ggatggccgg cggggcgcct
     8701 tctgctgcct gctggtggcc ggctatgcgg tgatgatcgc cttcttctcg cgggccgaca
     8761 cgttctactg gggggcgatc atgctgccct ggtatttcat cggctatgtg ctgctgccgc
     8821 gcgcggtgct gcagctgtgg ggggtggcgc gcgggaagcc cggttctttc gtgattggca
     8881 agaagccggc cagcctgtag agcgctggcc catacatctc acatcttcgc tggagaccgc
     8941 cacgtggccg cccaatacgc cttcgtcatg aagagcatga cgaaaacctt tcccggtgcg
     9001 caaaagccgg tgctcaacaa catcagcctg cagttctatc agggcgccaa gatcggcatc
     9061 gtgggcccca acggcgcggg caaatccacg ctgatgaaga tcatgggcgg gatcgacacg
     9121 gattacaccg gcgaggcctg ggcgggcgag aacatcaccg tgggctatct gccgcaggag
     9181 ccgcagcttg atcccaccaa gtcggtgctg gaaaacgtca aggatggcgc tcgcgagacg
     9241 gcggatctgg tcgaccgctt caacgagatc agcaacatca tggccgatcc gccggaagac
     9301 gccgatttcg acgcgctgat ggaagagatg ggcgagctgc agggcaagat cgacgcggtg
     9361 gacggctgga cgctggacaa ccagctggag atcgccatgg aagcgctgcg ctgcccgcct
     9421 tccgactggc cggtggaaag cctttcgggc ggtgaaaagc gccgcatcgc gctcacccgc
     9481 ctgctgatcc agaagcccga catcctgctg ctggacgagc ccaccaacca cctcgacgcg
     9541 gaaagcgtgg agtggctgga aaaccacctg aaggaatacc acggcgcggt gctgatgatc
     9601 acccacgacc gctacttcct cgacaatgtg gtgggctgga tcctcgagct cgaccggggc
     9661 aagtacttcc cctacgaggg caattattcc acctatctgg aaaagaagag ccagcgcctg
     9721 gcccaggaag accgcgaggc caccggccgc cagaaggcaa tcaacgacga gctggaatgg
     9781 atccgcgccg gcaccaaggg ccgccagacc aagagcaagg cccgtatcaa gaagttcgag
     9841 gaactggtcg ccagccagga aaaccgtggc cccggcaagg cccagatcgt tatccaggtg
     9901 ccagagcgcc ttggtggcaa ggtgatcgag gcgaagaaca tctccaaggc ctttggcgac
     9961 aaggtgctgt tcaacgacct gtcgttcctg ctgccgccgg gcggcattgt cggcgtgatc
    10021 gggccgaacg gcgcgggcaa gtccaccctg ttcaagatga tcaccggcca ggaaaagccc
    10081 gatagcggca cgatcgaaat cggcagcacc gtgcgcctgg gctatgtcga ccagagccgc
    10141 gaccatctgg acccgaccaa gaacgtctgg gaagaaattt ccgacgggct ggattacatg
    10201 aaggtcaacg gtttcgacac ctcgacgcgc gcttatgtcg gcgccttcaa cttcaagggc
    10261 caggatcagc agaagaacgt cggcaagctg tcgggcggtg agcgcaaccg cgtcaacatc
    10321 gccaagatgc tgaagcgcgg cggcaatgtg ctgctgctgg acgaacccac caacgacctt
    10381 gacgtggaaa cgctggcagc gctggaagag gcgatcgaga acttcgccgg ctgcgccgtg
    10441 gtcatcagcc acgaccgctt cttcctcgac cgccttgcca cgcacatcct tgcctttgag
    10501 ggcaatggcc atgtcgaatg gttcgagggc aacttcgagg cctatgagga agacaagcgc
    10561 cgccgcctgg gcgacgcggc tgaccgcgcc agcgccggcg cctacaagaa gctgacccgc
    10621 tgaggccagc ctggcgccag caagccggca tcgccggcct tcgatgacaa gcctgcatgg
    10681 ccggcgttcc ccgatgggag cgccggccat tcattttggc tatcctgaaa atgaacgcca
    10741 gccggccgca tggttcagct tggcgacagc ggatttcacc tagattgcag cttgtgaggt
    10801 gctacatttc agcgcctgcg gggtaggagg tgcgatgatg gccaggattt cttcttcttt
    10861 gcgcaagggc gccagcgccg ctttcgtggt ggccatgctg atggcctctg ccgcgccgat
    10921 ggcacaggcc cagcccggcc cgggcaacga acatcgtggc ggccagggcg gtggtcaggg
    10981 cggcggccaa ccccagggtg gcggcggcaa ccatggtggc ggccaacccc agggcggcgg
    11041 cggcaaccat ggcggtggcc aaccccaggg cggtggcggc aatcatggcg gcggtggcca
    11101 gccccagggc ggcggccagc ctcagggccg gccgcagggc ggtggccagc cgcaaggtgg
    11161 cggccagccc atgatgggcg gcggcaatcg cggtggcggc cagatggtga tgccgcatgg
    11221 cgtttccggc cagccgatga tgaacggtgg caaccgcccc ggcggccagc cgatgatgaa
    11281 cggcggcaac cgccccggcg gccagccgat gatgaacggc ggcaatcacc ccggcggcca
    11341 gcccatgatg aacggcggca atcgcggccc cgatgggcgc ggcgggccgg atgggcgcgg
    11401 cggccccgat ggccgtggct ggtcgcgcga ttggcgcaat gattcgcgct acgactggcg
    11461 cggctggcgc gagggcaacc gcgatgcctt ccatatcggc cgctattatc cgccctatcg
    11521 cggctatggc tatcgccggc tgggcgtagg cttcatgctc gatccgctgt ttttcgcttc
    11581 cagctactgg atttacgacc cctggttcta tcacctgccc gatgcctatg ggccctatca
    11641 ctgggtaagg tattataacg atgccctgtt ggtggatacg tcaaccggtc aggtggtaga
    11701 agtgctcaac gacttcttct ggtaacccct gccagaagcg gaacgccggt agagtggacc
    11761 ggccgatggc ccccgccagc cgctgccccg cggttgacgg gggcttttcc tttgcgccgg
    11821 gcttgggttg cgctagcctc cggggatgtt caccgccctg cgccctgccc gccccgccgc
    11881 cgaccgctcc gcgctggagc ggctgggcag cgccgtgcgc cagcggctgg atgctgatcc
    11941 tgccgcctgg cgcatcccgg tggaagcggc ggaaatctat gccatcggcc agttcctcag
    12001 cgcacaggaa tgcacccgct tcatcgccat ggtggacgag gtggcgcagc cctctgccac
    12061 tttcgatccc tccagccagc aaacctatcg caccagcttt tccggcgatg tggaccgcga
    12121 ggatcccttc gtgcggatga tcgagcggcg gatcgacgac ctgctgggca tagcgcccgc
    12181 atatggcgag acggtgcagg gccagcgcta tcgcccgggg caggagttca aggcccacca
    12241 cgactggttc tacaccgatg ccgcctattg gccgcaggaa aagcgccatg gcggccagcg
    12301 cagctggaca gcgatgatct acctcaacca ggtggaggca ggcggcgcca cggtgttccc
    12361 cgaccttggc gtctcggtaa cgccggagcc gggcatgctg ctggcctgga acaatgccac
    12421 gcccgatggc ctgcccaacc ccgccacgct gcatgcggca gagccggtgg ccagcggcgt
    12481 gaaatatgtg atcaccaaat ggtatcgcac ccggcgctgg ggagcgtgag cgagcgccag
    12541 ggcccgctga acgccccccg ggcggcttat ttgtccagcg cctcggcgat cagcgcgcgg
    12601 gtgttgccaa tgccataaag cgcgatgaag ctgcccatgc gcggcccctg cgcgctgccc
    12661 agcagcgttt catacagggc cttgaaccag tcgcgcagcg attcaaagcc atagtgcgga
    12721 tccttgccga tctcgtagac gatgttctgc aattcctcag ccgaggcatc gtcgctggtg
    12781 gcggccagtt cctcgtccag cgcggcaagg gcggcggcct cgttggcctc gggcttgcgg
    12841 cgctgcagcg tgggggcgat gaaatcgcgg ttgtaggcca gcgcgcaggt caccagcgtg
    12901 tcgagcgcgg ggtgggcctc ggcgcgggca tcctccacgt aattggccag atagctccac
    12961 acctgctcgc gcgtggccga ggcgcccagc acgcccacca ggttcagcag caggccatag
    13021 gtcaccggca gccgctcgcc ctcgcctgcg ccttcacccg cgccattggt gcgcagcagg
    13081 tgccacaccg ggttgcccag ctgctgctcc agcggctgga cggccagctt ttcacggaac
    13141 tgccaatatt cgtccaccgc gcgggcgatg atgccggggt gcaggctctt ggcgctcttg
    13201 ggctcgcgga agaggtagaa gccgaggctc tcctcgctgc cataggtcag ccattgctcg
    13261 atcgtcaggc cattgccctt cgacttggag atcttctcgc ccttctcgtc gaggaacaac
    13321 tcgtagatca gcccttccgg cttgcggccg cccagggcct gggcaatctt gccgctctgc
    13381 gtcaccgaat cggtgagatc cttgccgcac atttcgtagt ctacgcccag cgccacccag
    13441 cgcatcgccc aatccacctt ccattgcagc ttggcgccgc cctggaagat gcaatgctcg
    13501 atgatctggc ctccaggaac actggcatcc tcaaagcgga cgaggcccgc ctcggcatcc
    13561 accacttcca ccggcacctg caacaccacg ccgctggtgg gcgagacggg caggatgggc
    13621 gaataggtct tgcgacgctc ctcgcgcagg gtgggcagca tgatgcccat gatcttgtca
    13681 taggcgcgca gcacgccgcg cagggcctca tcgaaatcgc ccgcgttata gcgatcggcg
    13741 gccgacacga attcgtaatc gaagccgaag cgatcgagga aggcgcgcag catggcgttg
    13801 ttatgcgcgg caaaactgtc atgcgtggcg aagggatcgg gaatgcggct gagcggcttg
    13861 ccgagattgg ccgccagcag atcgccgttg ggcacattgt cgggcacctt gcgcagcccg
    13921 tccatgtcgt cggaaaaggc gacgaggcga gtggggtggc cgccggtcag cccttcatag
    13981 gcgcggcgca ccagcgtggt gcgcagcact tcctggaaag tgccgatatg cggcaggccc
    14041 gaagggccat agccggtttc gaacagcacc ggcacgaggt tgccatcgcc atcgcgtttg
    14101 ccctgcgggt aacgcttgac gagcttgcgg gcctcttcaa acggccaggc cttggacagt
    14161 tgcgcagcgg cgatcagttc attgtcggtc atgcgcggtg ctttgcgcgg gcgcgcaaga
    14221 tttcgcaagc gggaaggcgt ggatggcgcc cctgcccggc ccgcaatgcg tttactttgc
    14281 cgcgccaagg cgccatcacg cagcagtgac caccccgcct gcctcgcccc cgcctgcccc
    14341 ctcgctgccc gccccgctga gcctgccggc gctgcatgcc agccattccg gctgctggct
    14401 gcgcgaaaag ggcggcccca cgcgcccctg cgccaagggc gaggcgattg tggccgccgc
    14461 cgacacaccg ctgctgctgc tgaacgcgcc gctggtggcc agccggctgg gctatccgga
    14521 tctgtcgggg ctggatctgc tggagcttta tgccttcatc cacccggcgc gcttcatcgt
    14581 gcccaccgcg cgcgggctgg cgcaggcgct gggcattggt gcagccgagg ggggtgccgg
    14641 ctccacggaa accgagatca ccgatgccga tgtgcccgcc ctgctgcaac aggccgccgc
    14701 ctgcctgatc gccacctgcg ccgcgcccga ctggcccgag cgcgagggcg catggacggc
    14761 gctgcaggcg ctgacccggc tgcgctggcc ctgggcggcg ctgctggccc cccatatcgc
    14821 ccagcctgac cgggccgagc gctggctgtt cacccgcctg cccgaatggg aggaagcggc
    14881 cgaacgcccc cagcccgcac aagtgacgct ggacgagagc gatgtgctgg cgcgcctggc
    14941 cgccctcacc ggcgaagggg cggaacatcg ccccggccag caggcctatg ccgcggcggc
    15001 ggcgcgcatc tttgcccccc gcggccaccc caaggcgccg catatggtgc tggccgaagc
    15061 cggcaccggc accggcaaga ccctgggcta tctggcgccc tcctcgctgt gggcgcaggc
    15121 ggccggcggc acggtctggg tgtccaccta caccaaggcg ctgcagcgcc agctgcgccg
    15181 cgaaagccgc cgcgtgtggg ctgacagcca gccgggcccg gccccggtgg tggtgcgcaa
    15241 ggggcgtgag aactatctgt gcctgctgaa cctggaagat gcgctgcagg gcggcttcac
    15301 cggccggccg gcggtgctgg cccagctggt ggcgcgctgg gccgcctata gccgcgatgg
    15361 cgacatgatc ggcggcgatc tgccgggctg gctgggcacg ctgtttcgcc agcgcggcat
    15421 caccgcgctc accgaccggc gcggcgaatg cgtttatgcc ggctgcccgc attatcgcaa
    15481 atgcttcatc gagcgggccg cccgggccag cgccgaggcg cagctggtca ttgccaatca
    15541 tgcgctggtg atggtgaatg cggcgcgcgg caaggatgtg gcgcagcggc ccacgcgcat
    15601 cgtcttcgac gaggggcacc atgtgttcga ggcggccgac agcacctttg ccgcagacct
    15661 gaccggcgcc gagacgatcg agctgcgccg ctgggtgatc gggccggaaa agggcagccg
    15721 cgggcgccgc cggggcctgg ccgcgcgcct gtccgacatt gccagctatg acgaggaagg
    15781 cggccgcgcg attgccgccg cgcgcgaggc ggcagaagcc ctgcccggcg atggctggct
    15841 gggccgcgtg gtggagggcg agccttccgg cccgctggag gcgctgctgg cggcggtgcg
    15901 ggcgctggtt tatgcgcgcg atgaaagcgg cgggcaggaa gcgggctatg cgctggagac
    15961 agaagcggcg aacctcgatg gcgatgtggt ggaagcggcg caaaccgctc tggccgcgct
    16021 tggccagctg cgccgcccgc tgatccagct gggcctcagg ctggaagcgg tgatcgccga
    16081 cgggccggac tggctcgacg ggcagggccg cgcgcggatc gagggggcgc gccagtcgct
    16141 cacctggcgg gtggagctga tcggcgcctg ggaagccttg ctggggggcc tgggcggcgc
    16201 tgccgatccg gccttcgtcg actggctggc ggtggaccgg tccgacgggc gcgagttcga
    16261 tattggcatc caccgccgct ggctggaccc gatgaagccc tttgccgcca gcgtgctggc
    16321 cccggcccat ggcgtgatgc tgaccagcgc caccctgcgc gatggcaagc acgccggcgc
    16381 cgaagagggc tgggaggcgg ccatcgcccg ttccggcgcc ggctggctgg aggggcccgc
    16441 ccatctcgcc agcttcccca gccccttcga ttatgccgcc caagcccagg tgctgatcgt
    16501 gaccgatgtg cccaagggcg atatccccgc gctggcggcg gcctatgcgc ggctggtgga
    16561 ggcagcaggc ggcggcgtgc tgggcctgtt caccgccatt cgccggctgc gcgccgttta
    16621 tggccgcatt gccgataggc tggcccgcgc cggcctgccg ctttatgccc agcatgtcga
    16681 tccgatcgac accggcacgc tggtcgatat cttccgcgag gacccgcatg ccagcctgct
    16741 gggcaccgat gccctgcgcg acggggtgga tgtgccgggc cattcgctgc ggctggtggt
    16801 gatggaacag gtgccctggc ccaagccctc catcctccac cgcgcgcgcc ggctggccga
    16861 tgccgatggc ggccaggcgc atgacgaccg catcatccgc gcgcgcctgg cccaggcctt
    16921 tggccggctg atccgcagcc gcggcgaccg ggggcatttc gtcgtccttt ccgccgcttt
    16981 ccccagccgg ctgctcagcg cctttccgca aggcacgccg gtgctgcgct gcacgctgga
    17041 tgaggcttta caacgcatcg gcgggggtgt ctcacggccg caggagcacg cagccgacga
    17101 gaccgactcg ccgcctgccc cccacaacat gccctttccc ggatggttgc ctgattgatg
    17161 aagaagctcg gcctgttccg ccacgccaaa tccgactggt ctgaccagcg cgcgcgtgat
    17221 ttcgaccgcc cgctcaatgc ccgcggccgg ctgggcgcgc cgctgatcgg ccgccacatc
    17281 cgcgattatg ccgaggccga gcagttccgc tgggaccggg tgctggcctc gcccgccgtg
    17341 cgcagcgccg agacgatcga gctggccgcc gccggcgccg gcatggaagc ggcccgcagc
    17401 tttgccatca actgggaccg gcggatctat ctggccagtt ccgccgcgct gatggatgtg
    17461 ctgcgcgaac agagcgatgc gctggacgcg atcctgatgg tgggccacaa ccccgggctg
    17521 gaagatttga tcttcgacct ggtgcccgat gatggcagca gccccttgcg cgatgtggtg
    17581 gaggccaagt ttcccaccgc cgcctttgcc gtgctggaac tggcgatcgc gcattggcag
    17641 gatgtggaag agggctgcgc gcggctggtg catctgatgc gcccgcgcga tctcgatccg
    17701 gcgctggggc cggaattgca cgactgattg cacgactgat aggggcgccc gatgatcagg
    17761 cgctaaccgt ccagcgcgcg ggccagccgg tcgcgcagca ggcgcaggcc ggccagcagc
    17821 gcggcctgcg ctcccgcatc cgcgccgggc ggcggagcct ctgcggcggg cagcggctgt
    17881 tccaccgcac cctcggtggc agcggcccgc agctggcggc gggcgccggc cagggtgaag
    17941 ccctgcccat gcaccatggt gtggatctgc tgcagcaagg ccacatcggc cggccggtaa
    18001 taacgccgtc cgcccgccag cttcaccggg cgcagcgccg gaaactggct ttcccaatag
    18061 cgcagcacat gcgttttcac gcccagcagg cgcgccactt cgccgatgtt gcgcagggca
    18121 tcaggcgcct tggcatcggc cggcagggca gcgcctgccg cgctgcctga ggctgccccg
    18181 gatagtgccg gcgcggccgc cggcaccatg cccggcaggc cgcccgcatc aggccgcgcg
    18241 ccctgaacct tgcggctcag ctgcccgata cccgctgttt caacatctgg ctggcgcgga
    18301 aggtgagcac cctgcgcggg gtgatcggca cttccacccc ggttttggga ttacggccca
    18361 cgcgctctgt cttgtcgcgc agcaggaacg tgccaaaacc ggaaatcttg acgttttcac
    18421 cggcagccag cgcatcggac atgtgctgca agatggcttc caccatcgtc agcgattccg
    18481 tacgagacaa acccatcttg cgattgatgg cttctgccag atcagcgcgt gtcaacgtgt
    18541 taatggagcg catccttgcc cccctctgcc tagaatggcg accctcccga atctcggaga
    18601 tagcaaatcc tgccgagaaa acaacgcaga acacagggca tcagctgccc ttcggtgcca
    18661 tttcgggtca attcggcaaa aaggaggccc ctttgggcgc taaccattcc cgcttatata
    18721 cggatcaggc tggcacccca ggtgaaaccg ccgcccatcg cctccagcat aaccagatcg
    18781 cccggctgga tgcgcccgtc gcgcagcgcc cggtccagcg ccagcggcac cgaagcggcc
    18841 gaggtgttgg catgttcggc caccgtcatc accaccttct cggtgggcat gcccagcttc
    18901 ctggcggtgg cttccagaat gcgggcattg gcctggtgcg gcaccagcca gtcgatatcg
    18961 gcgggcgtga agccggcctc gtccagcact tcgcccagca cgctcgacag gttgaccacc
    19021 gcatggcgga acacttcgcg gcccttcatg cgcagcttgc ccaccgtgcc cgtggtcgac
    19081 ggcccgccat cgacaaacag cagctggttg tgcgcgccat cagcatgcag cttggtggcc
    19141 agcacgcccg gtgccgaggc atcctcgctc tcgcgcgctt ccagcacgat ggcgccggcg
    19201 ccatcgccaa acagcacgca ggtggtgcga tcttcccaat cgaggatctt gctgaaggtt
    19261 tccgcgccga tcaccagcgc gcgggtggcc atgccggtgc gcagcatcga atccgccacg
    19321 cccagcgcat agagaaagcc cgagcacacc gccgccacat cgaaagcgat gccgccattg
    19381 gcgcccagcg cctgctgcac gatcgtggcg gtggcgggga aggtttgatc cggcgtggcg
    19441 gtggccagca cgatcagatc cagcgaggcg ccctcaatgc cggcggcatc cagcgcggcg
    19501 cgggcggcgg cgatcgccag cgtgctggtg ctctccccct cgcccgcgac atagcgctgg
    19561 cggatgccgg tgcgctcgac gatccattcg tcggtggtgt ccaccagctc ggccatttcg
    19621 gcgttgctga caaggcggct gggcagggcg gagcccgtgc cgatcagaac actgcgacga
    19681 atcactgatg agcctcgagg gtaaccttat gcgccatgcg cagattttcg gcgccaagct
    19741 gctccagatc ggcagcaatg cgctgggtga tgttttcctg cagcagccgc gcggtcacgc
    19801 cgatggcatt ggccacgccc ttggcattgg cgctgccatg gcttttcacc accaccccgt
    19861 tcagccccag gaacaccgcg ccattgtggt tgttcgggtc gagatggtgc ttcagcagtt
    19921 cggtggcggg cttggaaatg aggaagccca gcttggaacg cagcgagctg gcaaaactgc
    19981 ggcgcagcag atcgccgacg aagcgggccg tgccctccat cgccttcagc gccacattgc
    20041 ccgagaaacc gtcggtcacc accacgtcca cttcgccgcg gcacagcttg tcggcctcgg
    20101 tgaagccgtc gaagcgcagc ggcagatcgg tggcgccgcg cagcacggcg gcggcatcgc
    20161 gcagttgctc ggtgcccttg atatcttccg tgccgatgtt gagcagccgc acgcggggcg
    20221 ctgcactatc gatcacgatg cggctgtagg ccgcgcccat gatggcaaac tgcaccagat
    20281 tgcgggcatc gcattcggtg ttggcgccaa ggtcgagcat gaccagatcg ctgtcggcca
    20341 gcgtgggcat cagcgcggcc agcgccggcc tatcgatccc cggcatggtg cgcagggcca
    20401 gcttggcgat cgccatcagc gcgccggtgt tgccaccgct gagcgcggcg cccgccttgc
    20461 cggatttcac cgcatcgacc gccaggccca tggaggtgac acgcgcgcgg cgcagcgcct
    20521 ggctgggctt ctcctcgccg ctgatcactt catcggcgtg gagaatatcc agcaggccct
    20581 gcgcctgtgc agtctgcaag cgggggtggc gcgccacttc tgcggcgatg cggggcctgt
    20641 cacccaccag cagcaggccg caggcaagcc cgcgttccac tgccagggca acgcccgcca
    20701 ccatggtgcg cacgccatcg tcgccgccca tcgcgtccac ggcgatacgc ggcacactca
    20761 tcagcgcatt ccccctgggg tcagatcgat cagaggccga cagccagaac ctcacggcca
    20821 ttgtaatggc cgcaggacgg gcacagattg tgcgggcgct tcagttcgcc acagttcgag
    20881 cactcgtgga aggcttcgac cttcagcgcg tcatggctgc gacgcatgcc gcggcggctc
    20941 ggggaagtct ttctcttggg gacggccatc gcggcacctg ttcctgaaaa tgtataaaaa
    21001 acaccaagcc gcgtcttgca ccatcagcag gcacggcggc agcgaaggcg cgcgtatagc
    21061 gaatttctcg tgcgttgcaa ggggcgtggc gctatcgcgg cgccagcctg cccgatcggg
    21121 cgcgccttga tgccctgtta taaggaaacc ccatgctgct gcccaccacc ttgtgcctgg
    21181 ccgctgccgc cgctgtcgtt aatttctggc tgggcctccg gattatgcgg ctgcgcatgg
    21241 cccgcaagat cttcatgggc gatggcgccg atccggcgat cgtggcgcgc atgcgggccc
    21301 aggccaattt catcgagaac acgccgatca cgctgattct cttcggcgcg gtggaagcgc
    21361 tgggcggggc ccacctgttc tggggcgggg cctggctggc gccgctgggg gcgatcttca
    21421 tgctgggccg ggtggcgcat gcctttggca tggatggccg cttcaagccc ggccggccga
    21481 tcggcatgct gacgacgctg ttcacccaga tcgtgctggc ggtggtggcg gtgctggcca
    21541 gcaccgggcg cctgtgagag ccaaaggggg cgttgcggcg cccccttgcc ttcagccata
    21601 gcggctgccg acaaagggat tcgtcagcat ttcgtggccg aaggtgctgc aagggccatg
    21661 gccggggata aaggtgatgt ccttgcccag cggccacagc ttttcggtga tcgaatcgat
    21721 cagctgctgc tggttgccct tggggaaatc cgtgcggccg atagagccgg caaacagcac
    21781 atcgcccacc agcgccagcc gcgagggcgc atgcacaaac accacatggc ccggcgtgtg
    21841 ccccgggcaa tgcaccacat cgaacaccag atcgcccacc gtcaccgtgt cgccatcgtg
    21901 cagccagcgg gtcggctcga agctcttgcc ctccagcccc atgcgggtgt tggggttgtc
    21961 cagcccctcg atccagaagc ggtcgccctc ttccgggcct tccagcggca gggccagctc
    22021 atcggccagc acgcccgcca gcccgcagtg atccatatgc ccatgggtga cgagcagctt
    22081 ttccagcgtc acgccatatt gcgccaccgc cgccttcagc ttgtccagat cgccgcccgg
    22141 atcgaccagc gcccccttca tcgttttggt gcaccacagc agcgtgcagt tctgctggaa
    22201 agcggtgacc gggatgatga cagcgcgcag cggcggctgg gcggcatctg tttgcggatc
    22261 ggtggaagcg gccatggcgg gggtgaaaga tatccttgcc cggcccgccc catgctggcc
    22321 ggttgcatgc agcagcgcta gcctatagcc cgggggcaat gcaattggcg ctgtgccggc
    22381 gcccccaatg aggacggatg atgcgcgccc ctgccctgtt cctggccctt gccgcgctgg
    22441 ccctgcccca gaccgccagc gctgctgctg ccccccatct ggccgagcaa ggctttggcg
    22501 atctcgcccg gccgctgccg ctgccttatg atggcgcggc cgatgcccat gccgcgattg
    22561 ccgcggcgcg cgcgcgggcg gcggccggcg gcaagctgct gctgatcgac cttggcggca
    22621 actggtgccc ggattgccgc attctggccg gcacgctggc cctgcctgat ctcgccctct
    22681 ttgtgcgcgc ccatttcgag gtggtgacgg tggatgtcgg ctatttcacc aaaaacctcg
    22741 atattgccga aagctatggg ctgggccggc ggccggcggg ggtgccggcg gtgctgatcg
    22801 tggagccgaa gagcaaccgg ctggtcaaca aggggcatga ggtggcgctg gccgatgccc
    22861 gcaccatgcg cccgcaggcc attgccgact ggctggccgg ctgggtgcgc tagagcccgg
    22921 ttggccagct agagccggcc gcctttccat accacgcggc cgatcggcac cacctcgcct
    22981 gcgggcaggt cgatcggcgg gtaggcgggg ttgtcgctga taagcagcag gcggcccgcg
    23041 ccactggccg ccacccgctt caccagcaga ctgtcgcccc ggcgcagcac atagacgcca
    23101 tcgcgcaagg gccggcggcg gcgatcgacg aggatctcgt cgccatcgcg caaggtgggc
    23161 tccatcgaat cgcccgccac cgccatggcc gccacctgcg ccggatccag cccctgcgcg
    23221 cgcaaccagg cggcggaaaa gcgcaggatg cccaccgccg cctcgccctg cggcaccgcc
    23281 cccggccctg ccgaggcatc cagcgccagg cgcgccacat ccacccagcg cccgggcccg
    23341 tgggaaattt cctgcccccc gcccagctcc gcctcatcca ccccgaagaa gcgggcgagc
    23401 ttgcggcgat ccgcctcatc cagcttgcgc gggctgccct tgcgcacgaa ttgctggaga
    23461 tagctggcat tgcgcccgat caacgccgac agcgccgcca ggctgacgcc gcgggcatcg
    23521 gccagcgcca acaggcggtt gcggggatcg gcgatcggca tgggggaaca gtatcatagg
    23581 atttttccta gacaagtagg aaatgccatg agaacaaaat agaaacatta ctttcgcgat
    23641 tcgctaaaag gaggggccga tgctgctgca caaaattgaa cagtttctgc gtcaatcggg
    23701 catgccatgg acgaaattcg ggcggatcgt ggcccatgat ccgcgcctgg tgggcgacat
    23761 gcgcaatggc cgcaacccgc gcgaggcgat gtgcgcgcgc atcgaggcgt tcatcgccgc
    23821 cggcgaagcc cccgctgccg aggagacccc ccatgcgttt tgatcgcaag cctgccggcc
    23881 agcatcgcgc cgatctgctg gcgcccttca gcgccgcccg cccggccagc accttgccgg
    23941 cggcaggccc gcaggcaagg ccgcggctgc gtcatccctc gctgcaggtg ctggaagcga
    24001 tgttgcggct ggccgggccc caggctcagc tgctttcaca tgccgagcgg ccctgggcca
    24061 gcgccacctt ctcgggcacg cggcacacga tcaccctgct gttcgatggc gccggggccg
    24121 tggccgccgc cgaggcgctg atcgccgccc tgcccgagca tgagttcacc atccgcggcc
    24181 agctggtggc cgatgccacg gtgagcgaag tgctgcacac gctgctgccg gccgagcgga
    24241 tggtggtgga ggcagacctg ctgctgctgg ccgaggggtg aggccggatc gccgcggggg
    24301 ggagatgccc cctcgcggca gcccggttca ggccagcttc cactcccaac ccagcggatc
    24361 gccgtccatc gcgtccaccc cttgtgcggc cagtgcatcg cgcagcgcat cggagcgggc
    24421 gaagtccttc tcggcgcggg cctgctggcg ctgggcgaga atggcctcga tctctgtctc
    24481 ggcaatgctg gccaccttgg ggcgcaggcg cagatcggcg cgggtgaggg agaggaggcc
    24541 aagccccagc accccatcca tcgcggcgat cgcggccagt ttctcgccgg ccggcacttt
    24601 cttcagcgcc gctgcctctt ccagcacggt gagcgcggtg ggggtggcca gatcatcgcc
    24661 gatggccgcc tcgaagcgct ccagcagggg ggcgaatttg gggccgggcg cggcgtgttc
    24721 tgccccttgc gcgcgcacct gtgccaccgc catcagcagc cgcttcagcc gggtgagcgc
    24781 ggccgacagc ccttcccagc tgaattccag ctcgctgcga taatgggcct gcaggcacat
    24841 caggcgatag gccagcgggt gatagccgcg atcgatcaac agctgcaggc gcaggaactc
    24901 gcccgagctc ttgctcatct tgccgctgcg ctcgacgagg aaattgttgt gcatccagat
    24961 tttggcgccg ctgttggccg gcacatccag cccgccggtg cagcagaagg cctggttctg
    25021 ggcgatctcg ttggggtggt ggatctcgcg gtggtcgatg ccgccggtgt ggatgtcgaa
    25081 gggaaagccc agcagctcgc ccgacatcac cgaacattcc agatgccagc ccggcgcgcc
    25141 gcggccccag gggctgtccc attccatctg gcgggtctcg cccggcgggg tcttgcgcca
    25201 gatggcgaaa tcggcggcat tgcgcttgcc atccaccttc tcgatgcggc cttcgccgtc
    25261 ctcggtggca gcgcgcgcca ggcggccata atcggcaacg gtcgagacat cgaaataaag
    25321 gccggtgggc agctcgtagc agtgccgcgc ggcgatcttt tcggcaaagg cgatcatgcg
    25381 cggcacataa tcggtggcga ccgaccattg cgccggctgg cggatgttca gcgcatcgat
    25441 atcggccttg aaggcggcgg tgtaatgggc ggcgatatcc cagatcgagc gggcctcgcg
    25501 ggcggcctgg cgctccagct tgtcttcgcc ggcatcggca tcatcggtca gatggccgac
    25561 atcggtgatg ttgatgacat gggtgagcct gtagcccttg tggttgagcg tgcggcccag
    25621 cacatcggca aacacatagg cgcgcatgtt gccgatatgg gcataattat agaccgtggg
    25681 cccgcaggtg tagacgcgtg cctcgcccgc atgggcgggg gcgaaatcct ccagctgccg
    25741 ggtcaggctg ttgaacaggc gcaagggggc ttgaaggggg atggcatcgc tcatccccgg
    25801 cggttggcaa gagcgccggc aaaggtcaag cccctgccgg cgcggcaggc cccttccgct
    25861 ttcaggcgaa accgcggaag ctcacgcttt cctccagcgg cacgcgcggg cgggcatagc
    25921 ggttaaccgg cgctgccgga tcgggccagc cgatggccag gccgcaccag aagaaatatt
    25981 cgccatcgtc tatcccgcag acctgcttga tcggcagcgg gaaatgggcc agcgattcct
    26041 gcgggcagga acccagcccc tcttccacca gcagcagcat caccgtttgc agccacagcg
    26101 ccgtgctggc ccaatgggcc gggctgagca cgcgcggcac gaaagtgaaa aggcaggccg
    26161 gcgcaccgaa gaattcgtaa ttcttgcggt tgtaggcggc gcggccttcc ttgtcctcgc
    26221 gggcgatgcc cagcgcatcc atccgctcgg ccatgatcgc ctcgcgccgg gcgatgaaat
    26281 gcgccggcgt ttcgggcgcg gagagtggat attcgggctg gctggccgga tctgccgcca
    26341 gcagcgcggc cgacagatcg gccagcggcc ggccggccag gatcaccccg cgccagggct
    26401 ggaggttggt gctggaaggg gccatgcgcg ccttttccag cacgcggcgc aaaatcgcct
    26461 cgtccaccgg ctgatcgagg aaggcgcgga tcgagcggcg ggtggccacc gcctcggaga
    26521 cggaaagggc agacatgggc gataaggctc ctggaatggg caggtgttgc cggaacggcc
    26581 gggggcgcgg gaagttcccg ctgccctcac ccgtcctcga acaggccggc gagctgctcg
    26641 atcatcgtgc cgcccagctg ctcgacatcc atgatcgtca ccgcccggcg gtaatagcgc
    26701 gtgacatcat ggccgatgcc gatcgccacc agctgcaccg gcgactgctt ctcgatccac
    26761 tcgatcacct tgcgcagatg cgcttccaga tagccggcgc tgttcacgct gagggtgctg
    26821 tcgtccaccg gcgcaccatc ggagatgacc atcaggatgc gccggtcttc cgggcgggcc
    26881 agcaggcggt tgtgcgccca cagcagcgcc tcgccgtcga tgttctcctt cagcagcccc
    26941 tcgcgcatca tcaggccaag gctctgcttg gcgcggcgcc agggttcatc ggcctgcttg
    27001 tagacgatgt gccgcagatc attgaggcgg cccggatgca cgggcttgcc ggcggccagc
    27061 caggcctcgc ggctttgccc gcccttccag gcgcgggtgg tgaagcccag gatctcggtc
    27121 ttcacgccgc agcgctccag cgtgcgggcc agcacatcgg cgctgatcgc ggcgatggaa
    27181 atgggccgcc cgcgcatcga gccggaattg tcgatgagca gggtgacgat cgtgtccttg
    27241 aactcgatgt cgcgctccac cttgtagctg agcgactggc ccggggcgat caccacgcgg
    27301 gccaggcgcg cggcatcgag caggccctct tcctgatcga aatcccagct gcggttctgc
    27361 tgggccatca gccggcgctg cagccggttg gccagcttgg tgacgatgcc ctgcagcccc
    27421 ttcagctgcg catcgagata ggcgcgcagg cgcttcagct cggcctcgtc gcacaggtca
    27481 tgcgcggcca ccacctcgtc gaagctttcg gtgaaaacct gatagtcgaa gccggccggg
    27541 tcgtccgtcc acgggcgatt gggacgcgtg ggcagcatgc cgtcctcggc atcctcgccg
    27601 ggctcggcat cctcggcgtc gtcgtcctgc tcgctctcgg cctcgtcctc gccatcctcg
    27661 ccgccttcct ggcggtcggc ggccatgtcc atcggctggg gagaatcctg ctggtcgtcg
    27721 ccgggctgtt cgtcctcgtt gtcgtcctca ctgtcgtcct cgccctcttc ctcgggctgg
    27781 tcgtcggtgg gggcggcctc ggtcagctcc agatgctgca gcatgtcgag cgccagcgcc
    27841 tggaaggcct tctggctttc cagattttcg gccagccggg caaaatcgcc gccggcccgc
    27901 ccctcgatcc acgggcgcac cagggcaacg ccggctgccg cgctttcggg gatgggctgg
    27961 ccggtgagat gttcgcgcag cagcagggca agggcgctgg ccatcggcac atcgccggcc
    28021 cgctcggcgc gggtgatcgg atcgccggcc acccgccgct ccaccgcggc ttccagattg
    28081 gcggcaacgc cggccatcgc ccgcgcgccc agcgcctcat agcgcacctg ctcgaccgca
    28141 tcgaaacagg cgcgcgcggt gggctcggcc ggcatgtggc ggccgtggag cgcggggttg
    28201 tgatggcgca gcttcagggc aaagctgtcg gccgcgccgc gcgcctcgtt ggcggcggcg
    28261 cgcggcaggc ggatgcccgg gctgggcacg cgaaactgcg tgccggcctg caccggagca
    28321 tcgccggtcc agttcacctc gacctcgggt tcgtgcgcca gggcgcgcgc ggcgccggtg
    28381 agcacatgtt tgaacctgtc gagcggggta tcctcggcca actgcttttc ccttggtttc
    28441 taaacggcga tcagagcttc tggccggtct tcgcccaatc ggcaaggaag ccttcgatgc
    28501 ccttgtcggt gaggacatgc ttgaacaggc ccttgatgac ggccggcggc gcggtcatca
    28561 catcggcgcc gatcttcgcg cattccagca catgcacgcc atggcggatg gaagcggcga
    28621 ggatctcggt ctcgtaggcg tagttgtcgt agatcaggcg gatgtcgcgg atgagatcga
    28681 ggccgtcaaa gccgttgtcg tcgtgccggc cgatgaaggg cgagatgaac gaggcgcccg
    28741 ccttggccgc cagcagcgcc tgattggccg agaagcacag cgtgacattg accttggtgc
    28801 catccgaggt gagcgccttg caggttttca ggccatcgat cgtcagcggc accttgatgc
    28861 acacattgtc ggcgatcttg cgcaggattt cggcctcgcg catcatgcct tcatggtcga
    28921 ggctgatcac ctcggccgac accgggccat cgacgagggc gcagatttcc ttggtcactt
    28981 ccaggaaatc acggcccgac ttggcgatca ggctggggtt ggtggtcacg ccatcaagca
    29041 ggccggtggc ggccagttcc ttgatgtcgg cgatttcggc ggtgtcgacg aagaatttca
    29101 tgaggcaggt tcctgctgct ggtatggccg gggccgggcc gccgcgcaac tctccttggc
    29161 gtgaggtgca cgcgggggat gggctgcgcg aaggggccgg cggttctgcc cccgcctata
    29221 ggcaggcttt gcgccgaacc caagccccca acgaaaggcg ctccacaaca gcacggctgg
    29281 cgcttgccga tccgcgcccg cctccccata gggaagagat ggccagccct gccccttccg
    29341 cctcgctgtt tcccgatgcc cagcccgatg cgcctgcccg cgtgcgcgtg ctggtgctga
    29401 atgcggcgct gggcgcgctg gattacaagg tgccgccagc catgcaggtg gcgccgggca
    29461 gcgtggtggt ggcgccgctg gggccgcgcc agatcctggg cgtggtgtgg gaggcagagc
    29521 ggctggaggc cggcgaagtg cccgcccaca agctgcgcgc cctgctggag gtgctgccgg
    29581 tgccgccgct ggccgccccc ttgcgccggc tgatcgaatg gacggcggat tattattgcg
    29641 cgccgctggc atcggtcgtg cggctggcgc tatcgtccag cgcggcgctg cggggcgggc
    29701 gcagcatgcg cgaataccgg ctggccgaa
//
LOCUS       DASRYE010000036         9447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_196104,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000036 DASRYE010000000
VERSION     DASRYE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196104"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFF98_01985"
     CDS             <1..435
                     /locus_tag="VFF98_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZU62426.1"
                     /translation="EALALFGLVDILIVNEHELARYAALAGLPAGNELAQAQGLLARA
                     EGAPRRAVVVTLGARGCLAVWREGHVAVPAFPVVPVDTIGAGDCFCGALAALLAEGAT
                     LEAALPIANAAAALCTQTPGAVPAMPTRAAVDAAVDAFVARG"
     gene            413..1045
                     /locus_tag="VFF98_01990"
     CDS             413..1045
                     /locus_tag="VFF98_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZU62427.1"
                     /translation="MLLLPVADLAPLLPPATTALVVVDAQVDFAAPHGRLGRAGVDLS
                     AIAPALARAEGLVAAARAAGVAVAFLRVVTRPQSDSPALQALMARRGMAGGEAICRAD
                     DGGADFYRLAPRSGDITVEKPLFDGFHATDLDAQLRARGIETLVLAGFSTDCCVAATA
                     GSGFHRGYHIFVAGDACATYEAQWQEAALAQMARHCALVVATGAIVAAWA"
     gene            1168..2373
                     /locus_tag="VFF98_01995"
     CDS             1168..2373
                     /locus_tag="VFF98_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62428.1"
                     /translation="MLCALSLLYFLLMATTFNALGLVLPFMVSELNWNWAEAGFGFTL
                     LGLSCGLTGLVPAVMIRRIGVSPTLLSGALLLVAGFVTLSLTHGVWAYDAGTVLLGLG
                     FSACGTVPGVYVISHSFERKSTAIGIYFSSGSLGSVAGPLLFYAVHAHASSWRAYWVI
                     AAVLALALGLFAASFTSVKYATIPNAGNAAPPDPNAWTARAALRTVPFYVIVGAYSVF
                     LLINTTIHSFAAQQLRELGFSDGGTASLLSAAALVGAVGSAVAGVVGEKLSARYLTIL
                     SLVAAAIGSLGLVIGAGWLGMGLFVVGLGIGLGFSYVSTAMLLLDFFGKEPNLELYSM
                     MCMISTSAALGPALGGLMRDRMGNFDAVFVACAGFGLVLAVMVLLLRRPQAPRGLVAA
                     PKVLVSEFG"
     gene            2471..4810
                     /locus_tag="VFF98_02000"
     CDS             2471..4810
                     /locus_tag="VFF98_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZU62429.1"
                     /translation="MEPDRPLRFTTNAFPKEQRHDAWRFALKRFSLVLAAADEASLYG
                     ELIHFRSAAGIDFVRITATAQSLEVDFSQQPDCFWMALVLGGSGRAEGPEGPIELGDG
                     DAICARGDKRALLHLAGEHRLLLMCLPTSLLGQRLKTPLPDNLRRIGAESGVARVFGG
                     MLRSLADTIVEITEDQARPIELALPEFLLASLLDNAPPKAMGGAAGLRAALLERIFQT
                     IEMRLADPNLNYQQVASEHGISPRYLQKLFESINDSFGHYVKVRRLERCRLDMRSPLH
                     VQKSISDILFQWGFNDSASFSRAFREQYGISPREYRKAPAGEEVAAPALRRGRPAGRE
                     ALPAPDAPTDLPADDAEAEADTAALAAPDHPVRHHHLPVSPRTIHWGYFSRNLRPALT
                     ISSGDFVTVETLTHHANDDHERMVEGDAGAESVYRWTAEGKAVERRGAGPMDASALGR
                     GPGEGFGVHICTGPIAVAGAMPGDVIEVRILDLKPRASGNPRFSGKAFGSNAATYWGF
                     HYQDLISEPRNREVITIYEVEAGGERPATAHAVYSFHWTPQVDPSGIRHERYDYPGVP
                     VDPATIERNYDILRNVEIPIRPHFGVIAVAPAHSGLVDSVPPGAFGGNLDNWRTGPGS
                     RVFLPVQVPGALLSLGDPHASQGDSELCGTAIECSMTALIQVIHHPAGDMKGTLRDLD
                     YPLIETDSEWVIMGFSHPDYLKELGEDAQSEIYKRSSIDAAMRDAFRKARRFLMTTKQ
                     LTEDEAISLISVGVDFGISQVANGNWGVHAVIRKALFTA"
     gene            complement(4922..6172)
                     /locus_tag="VFF98_02005"
     CDS             complement(4922..6172)
                     /locus_tag="VFF98_02005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU62430.1"
                     /translation="MLKRLFGALVAAAALCPAAHAAPADKLRADLADLVARHQIPCAE
                     VMIARHGRLLTHIRLGEADIDAHTLLPADAIFRFYSMSKPITSVAILMLAEEGRLRLD
                     DPVARWLPAFGQMRVYAGGGADDMRTVPANRPITIEDLLLHRGGLTYPFMGAGPVQRY
                     YRSHGVQRDTPVGRQPGDAPPAQDLAQLVERLGAAPLLHQPGEKWDYSYSTTVLGAVV
                     EKASGQSLDAFLRQRIFAPLEMKDTGFVITPAQLPRFVALTTATAQGLATSETAAASE
                     YRDPARLRDGGGALAGTTRDYLHFAEMLANGGSWHGRRLLNARSVAAMFTPRLPTGGS
                     GLEDTRFGYGLAIGDAHTAAIGGLPVGAGSWSGSGNSYFFADRRRGLAVVFMTSELTP
                     GPFAARETATRQLVDRGVESLLAP"
     gene            complement(6166..6975)
                     /locus_tag="VFF98_02010"
     CDS             complement(6166..6975)
                     /locus_tag="VFF98_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZU62431.1"
                     /translation="MPLPLTIRPLTRRIGAEIFGLDLALALAPEDVAAVQAALAAHKV
                     VFFRDQQLSPAGLVELGRRFGDLVLHSAVAGLADHPEVVAIHADADSTFVAGEDWHSD
                     LSEHAAPPMGSILYMHTLPETGGDTLFACMEAAYDALSPAMQAFLDPLTALHDANPVY
                     REIFPLVEKTYPKHTHPVVRRHPVTGRKGIFVNASKTTRIHELAKGESDALLAFLFDH
                     VKNPNFQTRFRWQQGSVAFWDNRCTQHFAVWDYFPDVRSGWRVTIAGEAPC"
     gene            complement(7060..8148)
                     /locus_tag="VFF98_02015"
     CDS             complement(7060..8148)
                     /locus_tag="VFF98_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZU62432.1"
                     /translation="MALICEHGTRRNDVRPGALHVLPATPETIHWGYFSPEIKPALTV
                     KSGDLIQAQAVTHHAGDDPALMFDESIRRIFAEVDPATRAPGCHIMTGPIFVEGAEPG
                     DMLEVRYFQMVPRFNYGSNLQASWGHLYEEFGKTERVTIYQLDPNANTASALYAYDIE
                     GDYGAPGRITHCPECDRQAALPGISIPARPHLGTAGVAPAVNSPVSTIPPGLHGGNID
                     NWRIGAGARMYYPVQVKGGLFSIGDPHVSQGDGEISGTAIEASLDVLFQIILRKDFSF
                     PSPLLETQDCWIVHGFGADLDAAMKGASLDMIHLLSEHQGLSRTDAYSLMSVAADFGV
                     TQVVDGTQGIHCRIDRGIFPNKGSVRDL"
     gene            complement(8206..8997)
                     /gene="ygiD"
                     /locus_tag="VFF98_02020"
     CDS             complement(8206..8997)
                     /gene="ygiD"
                     /locus_tag="VFF98_02020"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HZU62433.1"
                     /translation="MHDRPRLPLLFFGHGSPMIALESNSTTATWRAIAQEVGRPRAIL
                     CISAHWLTRGTAVTAMASPPTIHDFGGGFPRALFAKQYPAPGDQRLASRVRDLLAPLP
                     VALDNDWGLDHGTWSVLTHAWPLADVPVVQLSMDVRKTPQEHWQIGQALRPLRDEGVL
                     IIGSGNIVHNLPAMDWHNPEAEPYPWAQRFNAAILSAVVEDRPQAVIDFAALGEDAQL
                     SCPTPDHFWPLLYVLGARHPGEAARLAPDFIQHKSLGMTSIFIGA"
     gene            complement(9015..>9447)
                     /locus_tag="VFF98_02025"
     CDS             complement(9015..>9447)
                     /locus_tag="VFF98_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU62434.1"
                     /translation="GRFYAEVFGWQNDARPEGVYHRMVPGGQFRNPDGTDSAIGNLHI
                     GIFKAENARPHPKPEGVAPRDLATSGRKPRVWILIDDEDSAERILAAAEERGASILWR
                     NHYWAEFNGYNHAFADPWGNEIILWGKAGPNPAIPADFTRE"
BASE COUNT         1406 a         3071 c         3364 g         1606 t
ORIGIN      
        1 gaggcgctgg cgctgttcgg ccttgtcgat atcctcatcg tcaacgagca tgagctggcc
       61 cgctatgccg cgctcgccgg ccttcccgcc ggtaatgagc tggcgcaggc gcagggcctg
      121 ctggcgcggg cggaaggggc gccgcgccgg gcggtggtgg tcacgctggg cgcgcgcggc
      181 tgcctggccg tgtggcgcga ggggcatgtg gcggtgccgg ccttcccggt ggtgccggtg
      241 gatacgatcg gcgcgggcga ttgcttctgc ggcgcgctgg cagcgctgct ggccgaaggc
      301 gccacgctgg aggcggcgct gcccatcgcc aatgcggccg cggccctgtg cacgcaaaca
      361 cccggcgccg tgcccgccat gcccacgcgt gcggcagtgg atgccgcagt ggatgctttt
      421 gttgcccgtg gctgatctgg cgccgctgct gccgcctgcc accacggcgc tggtggtggt
      481 ggatgcgcag gtggattttg ccgcgcccca tggccggctg ggccgggcgg gggtggatct
      541 gtcggccatt gcgccggcgc tggcgcgggc agaggggctg gtggcggcgg cgcgcgcggc
      601 cggggtggcg gtggcgttcc tgcgcgtcgt cacccggcct cagagcgatt cgccagcgct
      661 gcaggcgctg atggcccggc gcggcatggc tggcggcgag gcgatctgcc gggccgatga
      721 tggcggcgcc gatttctatc gcctcgcccc gcgctccggc gacatcaccg ttgaaaagcc
      781 cctgttcgat ggcttccacg ccactgatct tgatgcccaa ttgcgcgccc ggggcataga
      841 gacgctggtg ctggccggct tttccaccga ttgctgcgtg gcggccacgg ccggctcggg
      901 ctttcatcgt ggataccata tctttgtcgc cggcgatgcc tgcgccacct atgaggcgca
      961 gtggcaggaa gccgccctgg cgcagatggc gcgccattgc gcgctggtgg ttgccacagg
     1021 ggccatcgtt gcggcctggg cgtgatgcgg ggccgaacaa tcagggcgcc cctgatcaag
     1081 acctgaccgc cacgctgggc cacactgcgg gcaggtataa ggaaggaccc ggctccaatg
     1141 gtcttcgagg gacgcgcccg tttgatagtg ctttgcgcac tcagcctgct ctattttctg
     1201 ctgatggcca ccaccttcaa cgcgctgggg ctggtgctgc ccttcatggt gagcgagctg
     1261 aactggaact gggcggaggc cggtttcggc ttcaccctgc tgggcctgtc ctgcgggttg
     1321 accgggctgg tgccggcggt gatgatccgc cgcatcggcg tcagccccac tctgctgagc
     1381 ggcgcgctgc tgctggtggc gggctttgtc accctctcgc tcactcatgg cgtttgggcc
     1441 tatgatgccg gcacggtgct gctggggctg ggcttttcgg cctgtggcac ggtgccgggg
     1501 gtctatgtca tctcccattc cttcgagcgc aaatccaccg ccatcggcat ctatttttcc
     1561 agcggcagcc tgggttccgt ggcagggccg ctgctgttct atgcggtgca tgcccatgcc
     1621 agcagctggc gcgcctattg ggtgatcgcg gcggtgctgg cgctggcgct gggcctgttc
     1681 gcggccagct tcacctcggt gaaatatgcc accatcccca atgccggcaa cgccgccccg
     1741 cccgatccta acgcctggac cgcgcgcgcg gctctgcgca cggtgccgtt ctacgtgatc
     1801 gttggcgcct attcggtgtt cctcctcatc aacaccacga tccacagctt tgccgcccag
     1861 caactgcgcg aactgggctt ttccgatggc ggcaccgcca gcctgctgag cgcggcggcg
     1921 ctggtggggg cggtgggctc ggccgtggcg ggcgtggtgg gcgagaagct gtcggcgcgc
     1981 tatctcacca tcctctcgct ggtggcggcc gcgatcggct cgctggggct ggtgattggc
     2041 gcgggctggc tgggcatggg gctgtttgtc gtcgggctgg ggatcgggct tggcttctcc
     2101 tatgtgtcca ccgccatgct gctgcttgat ttcttcggca aggaacccaa tctcgaactc
     2161 tattcgatga tgtgcatgat ctccacctcg gcggcgctgg ggccggcgct gggcgggctg
     2221 atgcgcgaca ggatgggcaa tttcgatgcg gtgttcgtgg cctgtgccgg ctttggcctg
     2281 gtgctggcgg tgatggtgct gctgctgcgc cggccgcagg cgccgcgcgg gctggtggca
     2341 gcgcccaagg tgctggtttc cgaattcggc tgacagggcc cgctgcccgc ccttgaccag
     2401 ccatgacgcc ttcgcctagc ctgctgccac cggcgccaga ccggaccctg gggtagcggg
     2461 cgccgggcag atggagccgg accgaccctt gcgctttacc accaacgcct ttcccaagga
     2521 gcagcgccac gatgcctggc gctttgccct caagcgcttt tcgctggtgc tggccgccgc
     2581 cgacgaggcc agcctctatg gcgagctgat ccatttccgc agcgcggcgg gcatcgattt
     2641 cgtgcggatc accgccacgg cccagtcgct ggaggtggat ttctcgcagc agcccgattg
     2701 cttctggatg gcgctggtgc tgggcggcag cggccgggcg gaagggccgg aggggcccat
     2761 cgagctgggc gatggcgatg ccatctgcgc gcgcggcgac aagcgggcgc tgctgcatct
     2821 ggccggcgaa caccggctgc tgctgatgtg cctgcccacc agcctgctgg gccagcgcct
     2881 gaaaaccccg ctgcccgaca atctgcgccg gattggcgct gaaagcgggg tggcgcgggt
     2941 gtttggcggc atgctgcgct cgctggccga taccatcgtc gagatcaccg aggaccaggc
     3001 gcggccgatc gagctggcgc tgcccgaatt cctgctggcc agcctgctcg acaatgcccc
     3061 gcccaaggcg atggggggcg cggcgggcct gcgcgcggcc ctgctggagc gcatcttcca
     3121 gaccatcgag atgcgcctgg ccgatcccaa cctcaactat cagcaggtgg ccagcgaaca
     3181 cggcatttcg ccgcgctatc tgcaaaagct gttcgaatcg atcaacgaca gtttcggcca
     3241 ttatgtgaag gtgcgccggc tcgaacgctg ccggctcgac atgcgcagcc cgctgcatgt
     3301 gcaaaaatcg atctcggata tcctgttcca gtggggcttc aacgattcgg cctcgttcag
     3361 ccgggctttc cgcgagcaat atggcatttc cccgcgcgaa taccgcaagg cgccggcggg
     3421 cgaggaagtg gcagcgccgg ccctgcgccg gggccgcccg gccggccgcg aggccctgcc
     3481 cgcgcccgat gcgcccaccg acctgccggc cgatgacgcc gaggccgagg ccgacaccgc
     3541 ggccctcgcc gcccccgatc atccggtgcg ccaccatcac ctgccggtca gcccgcgcac
     3601 gatccactgg ggctatttca gccgcaacct gcgccccgcg ctgacgatca gctcgggcga
     3661 tttcgtcacg gtggaaaccc tcacccacca cgccaatgac gatcacgagc gcatggtgga
     3721 aggcgatgcc ggggccgaaa gcgtctaccg ctggaccgcc gagggcaagg cggtggaacg
     3781 gcgcggcgcc gggccgatgg atgccagcgc cctgggccgc ggcccgggcg aaggttttgg
     3841 cgtgcacatc tgcaccgggc ccattgcggt ggccggcgcc atgccgggcg atgtgatcga
     3901 ggtgcgcatc ctcgatctga agccgcgcgc cagcggcaat ccgcgctttt ccggcaaggc
     3961 ctttggcagc aatgccgcca cttactgggg ctttcactat caggatctga tcagcgagcc
     4021 gcgcaaccgc gaggtgatca ccatctatga ggtggaagcc ggcggcgagc ggccggccac
     4081 cgcccatgcc gtctattcct tccactggac cccgcaggta gacccttccg gcatccgcca
     4141 cgagcgctac gactatcccg gcgtgccggt ggacccggcc accatcgagc gcaactacga
     4201 catcctgcgc aatgtggaga tccccatccg cccgcatttc ggcgtgatcg ccgtggcgcc
     4261 ggcccattcc ggcctggtgg acagcgtgcc gcccggcgcc tttggcggca atctcgacaa
     4321 ttggcgcacg gggccgggat cgcgcgtgtt cctgccggtg caggtgcccg gcgcgctgct
     4381 gtcgctgggc gatccccatg cctcgcaggg cgatagcgaa ttgtgcggca cggccatcga
     4441 atgctcgatg acggcgctga tccaggtgat ccaccacccc gccggcgaca tgaagggcac
     4501 gctgcgcgat ctcgattatc cgctgatcga gaccgacagc gaatgggtga tcatgggctt
     4561 ttcccacccg gattatctga aggagctggg cgaggatgcg cagagcgaga tctacaagcg
     4621 ctcgtccatc gatgcggcca tgcgcgatgc cttccgcaag gcccgccgct ttttgatgac
     4681 caccaagcag ctgaccgagg atgaggcgat ctcgctgatc tcggtggggg tggattttgg
     4741 catctcgcaa gtggccaatg gcaattgggg cgtgcatgcg gtgatccgca aggcgctgtt
     4801 cacggcgtga ggatacggcc ccggcccact cccccacccg gccaccctta gggtactatc
     4861 gttgggtggc cgggtggggg agtgggccgg ggcgccgcgc ccgcaagggc ggccagcaaa
     4921 gtcagggcgc cagcaggctt tccacgccgc ggtccaccag ctggcgggtg gcggtctcgc
     4981 gcgccgcgaa ggggccggga gtcagctcgc tggtcatgaa caccacggcc aggccacgcc
     5041 ggcgatcggc gaagaaatag ctgttgcccg agcccgacca gctgcccgcc cccaccggca
     5101 gccccccgat cgccgccgta tgcgcatcgc caatcgccag cccatagccg aagcgcgtgt
     5161 cttccagccc gctgccgccg gtgggcaggc gcggcgtgaa catcgccgcc acgctgcggg
     5221 cgttgagcag ccggcgcccg tgccagctgc cgccattggc cagcatttcg gcaaaatgca
     5281 gatagtcgcg cgtggtgccg gccagcgcgc cgccgccatc gcgcaggcgg gcagggtcgc
     5341 gatattcgct ggcggcggcc gtctcgctgg tggccaggcc ctgggcggtg gcggtggtca
     5401 gcgcgacaaa gcgcggcagc tgcgcggggg tgatgacgaa acctgtgtcc ttcatctcca
     5461 gcggggcgaa gatgcgctgg cgcaggaagg catcgaggct ttgcccgctg gccttttcca
     5521 ccacggcgcc cagcacggtg gtggagtagc tgtagtccca cttctcgccc ggctggtgga
     5581 ggaggggcgc tgcccccagc cgttccacca gctgcgccag atcctgtgcc ggcggcgcat
     5641 cgcccggctg gcggcccacc ggcgtgtcgc gctgcacgcc atggctgcgg taatagcgct
     5701 gcaccggccc tgcgcccatg aagggatagg tgagcccgcc gcgatgcagc agcagatcct
     5761 cgatggtgat cggccggttg gcgggcacgg tgcgcatgtc gtctgccccg ccgccagcgt
     5821 aaacgcgcat ctggccaaag gccggcagcc agcgcgccac cggatcatcc agccgcaggc
     5881 ggccctcttc ggccagcatc aatatcgcca ccgaggtgat cggcttcgac atcgaataga
     5941 agcggaagat ggcatcggcg ggcagcagcg tgtgggcatc gatatcggcc tcgcccaggc
     6001 ggatgtgggt gagcagccgg ccatggcggg cgatcatcac ctcggcgcag gggatctggt
     6061 ggcgggcgac gagatcggcc agatccgcgc gcagcttgtc cgccggcgcc gcatgggcgg
     6121 ccgggcacag ggccgcggcg gcgacaaggg ccccgaacag ccgcttcagc atggcgcttc
     6181 tccggcgatt gtcacccgcc agcccgagcg cacatcgggg aaatagtccc acaccgcgaa
     6241 atgctgggtg cagcggttgt cccagaaggc gacggagcct tgctgccagc ggaagcgcgt
     6301 ctggaaattg gggttcttca catggtcgaa caggaaggcg agcagggcat cgctttcgcc
     6361 cttggccagt tcgtggatgc gggtggtctt ggaggcgttg acgaagatcc ccttgcgccc
     6421 tgtcaccggg tggcggcgca ccaccggatg ggtgtgtttg ggataggtct tttccacaag
     6481 agggaagatc tcgcggtaga cagggttggc atcatgcagc gccgtcagcg gatcgaggaa
     6541 ggcctgcatg gccggcgaga gcgcgtcata ggccgcttcc atgcaggcaa acagcgtgtc
     6601 gcccccggtc tcgggcaggg tgtgcatgta gaggatggag cccatcggcg gggcggcatg
     6661 ttcggaaagg tccgaatgcc agtcctcgcc ggccacgaaa gtgctgtcgg catcggcgtg
     6721 gatggccact acctcggggt gatcggcaag gccggcaacg gcgctgtgca gcaccagatc
     6781 gccaaagcgg cgccccagtt ccaccagccc ggccgggctc agctgctgat cgcggaagaa
     6841 caccaccttg tgggcagcga gcgcggcctg caccgcggcc acgtcttccg gcgccagcgc
     6901 aagggccaga tccagcccga agatttccgc cccgatgcgc cgggtgagcg ggcggatggt
     6961 gaggggaagg ggcatgcgat gctcctttgc aggggccggg ggcgcggcag gcgcctttag
     7021 tgcgacccga aggggcctgc cgccggggca aacggggggt tacagatcgc gcacgctgcc
     7081 cttgttgggg aagatgccgc ggtcgatccg gcagtggatc ccttgcgtgc catccaccac
     7141 ctgggtcacg ccgaaatcgg cggcaacgct catcagcgaa taggcatcgg tgcgcgagag
     7201 gccctgatgt tcgctcagca ggtggatcat gtcgaggctg gcgcccttca tcgccgcatc
     7261 gagatcggcg ccgaagccgt ggacgatcca gcaatcctgg gtttccagca gcggcgaggg
     7321 gaagctgaaa tccttgcgca ggatgatctg gaacagcaca tcgagcgagg cctcgatggc
     7381 ggtgccggaa atctcgccat cgccctggct gacatgcgga tcgccgatgg agaacagccc
     7441 gcccttcacc tgcaccggat aatacatgcg cgcgccagca ccaatgcgcc agttgtcgat
     7501 attgccgcca tgcaggcccg gcggaatggt ggagacaggg ctgttcaccg ccggcgccac
     7561 gcccgccgtg cccagatgcg ggcgggcggg gatggaaatg ccgggaaggg cggcctgccg
     7621 gtcgcattcc gggcaatggg tgatgcgccc cggcgcgcca taatcgccct cgatgtcata
     7681 ggcataaagc gcgctggcgg tgttggcgtt gggatccagc tgatagatcg tcacgcgctc
     7741 ggtcttgccg aattcctcgt agaggtggcc ccagctggcc tgcaggttgg agccatagtt
     7801 gaagcgcggc accatctgga aatagcgcac ttccagcatg tcgccgggtt ctgcgccttc
     7861 cacgaagatg gggccggtca tgatgtggca gcccggcgcg cgggtggccg ggtccacctc
     7921 ggcgaagatg cggcggatgc tttcgtcgaa catcagtgcc gggtcgtcgc cggcgtggtg
     7981 ggtcacggcc tgggcctgga tcaggtcgcc gctcttcacg gtcagcgccg gcttgatctc
     8041 gggcgagaaa tagccccagt ggatggtttc gggggtggcg ggcagaacat gcagcgcgcc
     8101 gggtcgcaca tcgttgcggc gggtgccatg ctcacagatc aatgccatgt gtcctcctgt
     8161 gcctcatgcg tgtcgcgcga agggcgctgc tgaagagttg cgcgctcagg cgccgatgaa
     8221 gatgctcgtc atgccgagcg acttgtgctg gatgaaatcg ggggcaaggc gggcggcctc
     8281 gccggggtgg cgcgcgccca gcacatagag cagcggccag aaatgatcgg gcgtggggca
     8341 ggaaagctgc gcatcttccc ccagcgcggc aaaatcgatc accgcctggg gccggtcttc
     8401 caccacggcg gaaaggatgg ccgcattgaa gcgctgcgcc caggggtagg gttctgcctc
     8461 ggggttgtgc caatccatgg ccggcaggtt gtgcacgata ttgccgctgc cgatgatcag
     8521 cacgccctca tcgcgcaagg ggcgcagcgc ctggccgatc tgccagtgtt cctgcggggt
     8581 tttgcgcaca tccatgctca gctgcaccac cggcacatcg gccagcggcc aggcatgggt
     8641 gagcaccgac caggtgccat gatcgagccc ccagtcgtta tcgagcgcca ccggcagcgg
     8701 cgccagcaga tcgcgcaccc ggctggccag ccgctggtcg ccgggcgcgg gatattgctt
     8761 ggcgaacagc gcgcggggaa agccgccgcc aaaatcatgg atggtgggcg ggctggccat
     8821 ggcggtgacg gcggtgccgc gcgtcagcca gtgggccgag atgcacagga tcgcccgcgg
     8881 ccgccccact tcctgggcga tggcgcgcca ggtggcggtg gtgctgttgc tttccagcgc
     8941 gatcatcggg ctgccatggc cgaagaagag gagcggaaga cggggcctgt cgtgcatcgt
     9001 gcctgtcctg cggcttattc gcgggtgaaa tcggcgggga tggcggggtt gggcccggcc
     9061 ttgccccaca ggatgatctc attgccccag ggatcggcaa aggcgtggtt gtagccgttg
     9121 aactcggccc agtaatggtt gcgccacagg atagaggcgc cgcgttcctc ggcggcggcc
     9181 aggatgcgct cggcgctgtc ctcgtcgtcg atcaggatcc acacgcgcgg cttgcgcccg
     9241 ctggtggcca gatcgcgcgg ggccacgcct tccggtttgg ggtgggggcg ggcgttctcg
     9301 gccttgaaga tgccgatgtg caggttgccg atggcgctgt cggtgccatc gggattgcgg
     9361 aactggccgc cgggcaccat gcggtgatag acgccttcgg ggcgggcatc gttctgccag
     9421 ccgaacacct cggcatagaa acggccg
//
LOCUS       DASRYE010000037         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_213914,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000037 DASRYE010000000
VERSION     DASRYE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213914"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFF98_02030"
     CDS             complement(<1..531)
                     /locus_tag="VFF98_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62435.1"
                     /translation="MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRAL
                     EDLLGVVLFRRTAKGLELTAEASAGLEPLRAGFLQFEESVQAMQAGQSAHVFTLAAPR
                     EIVAPWLAARLAAYRALHPDVRIALVDGEGTDFAEANLDLAIRLAEGPGELEGAQLAP
                     ALRVQVGDAAAGWIGWP"
     gene            complement(560..973)
                     /gene="apaG"
                     /locus_tag="VFF98_02035"
     CDS             complement(560..973)
                     /gene="apaG"
                     /locus_tag="VFF98_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZU62436.1"
                     /translation="MKELFQHAATTAGVTVRVAVNFLPEQSRIEAGKWFWVYHIRIEN
                     DSARTMQLVTRHWRITDGHGAVSLVDGEGVVGEQPVLRPGQSHDYVSGCPLATPNGSM
                     EGHYTFHLTDAAPGEEIDLRVAIPFFPLAAPATAG"
     gene            1123..1608
                     /locus_tag="VFF98_02040"
     CDS             1123..1608
                     /locus_tag="VFF98_02040"
                     /inference="COORDINATES: protein motif:HMM:NF042720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6628 family protein"
                     /protein_id="HZU62437.1"
                     /translation="MPHPSARHPLSPAETPDPLAACLPLPMPAHEDARMLMIAARRMA
                     VHGLHDASAALLVLRHLGIHFRQPLVLMRAFMLELSDATRRPIMVAPCCTPRLTRDEG
                     DILATWSAASHDPAAAMAHLGRLTGGPAPAALLTTGAALANALANAGRALPPWPGAPA
                     D"
     gene            complement(1617..2825)
                     /locus_tag="VFF98_02045"
     CDS             complement(1617..2825)
                     /locus_tag="VFF98_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU62438.1"
                     /translation="MKRTTGQDRTITSKWRQATRAVRGGTWRSEQGETSEAIFMNSGY
                     CYEDAETPAARFAGQAPGMTYSRTQNPSVQMLEERIALLEGAEACRAQASGMAAMSSV
                     LLCQLSAGDHMVIARAAFGPTRWVTDNLLPRFGIAATPVDGRDLDQWKAAIRPNTKVF
                     FFETPANPTMDIVDIRAVSDLAHEHGITVVVDNAFASSALQRPLDHGADVVAYAATKL
                     MDGQGRVLAGAVAGTADFINNKLLPFQRNTGNTLSPFNAWIVLKGLETLDLRANAQAA
                     AAGEVARFLEGRVPSLLYPGLPSHPQHALAMRQMKAAGSIFSFEVAGGREAAMAVLNA
                     LELIDISNNIGDTKSLMTHPASTTHSSLTPETRAAMGVSEGMLRISVGLEDVADLTED
                     LDQALRSIGL"
     gene            2923..4236
                     /locus_tag="VFF98_02050"
     CDS             2923..4236
                     /locus_tag="VFF98_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZU62439.1"
                     /translation="MKAFLARSPARQRDPLGWCAALALAFAALAGWRLGTPGRIYFDE
                     VHYVPAARALLALIPANPEHPLFAKLAIAAAIATLGDQPAVWRLPSLVAGAIGLFAFG
                     RALWWASGRRFATIAGMVLLASDFAWFIQSRIAMLDMVMAGLGLVALGLVAGAIGLPQ
                     GAPAWQRRWRLAGAGLALGLAMGAKWSVLPLCAGLVLAVLAARALAIWRMGGETAATL
                     PIPGISLAELALWLISLPLVAYAATFTPAFFYHSQALAPTIPGLVAWHRYMVALQASV
                     IKPHPYQSHWWQWVIDWRAIWYLYEPVDGIQRGIVLIGNPVSMLAGLPALVWCLWAGW
                     RGRRLDAAAAVGFYALTLGLWVVAHKPVQFYYHYLLPGTFLMAALALALDDIWQAGGP
                     LRWAAPALVAAAVAVFAWFYPIIAAMPLHHGVMSFEDWMWLDSWR"
     gene            complement(4302..>5393)
                     /locus_tag="VFF98_02055"
     CDS             complement(4302..>5393)
                     /locus_tag="VFF98_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU62440.1"
                     /translation="RKNLRPLVERLAKALGDIRWEAIIVDDNSRDGTADEARAINLED
                     PRVRCIQRIGRRGLASAAIEGMCATAAPIVAVMDADHQHDPNLLPGMLAAIESGGYDL
                     AYASRFAEGASTEEWGRPDRVKASGFANALARKVTGVDLSDPMSGYFMLRAETLRADA
                     HRLSGVGFKILLDILATVERPLRVKEFPLNFAARAEGESKLDRTVVFEFLVGLYDKWL
                     GRFIPTRFALFGTVGALGVVVQLGVLWVMLHMVLRERFVVSAWTNDMRFNIGNTVAAL
                     AAMTFNFVLNNELTYSDKRLRGLVPVLRGWGKFALTCSFGLLTNVATAAALKRMHFHD
                     VVAVLVGIVLGSVWNFALSSKFVWGKYGN"
BASE COUNT          816 a         1837 c         1840 g          900 t
ORIGIN      
        1 gggccagccg atccagccgg ccgccgcatc gcccacctgc acccgcaggg ccggcgccag
       61 ctgcgcgccc tccagctcgc ccgggccttc tgccaggcgg atggccagat caaggttggc
      121 ctcggcaaaa tcggtgccct cgccatccac cagggcaatg cgcacatccg gatgcagcgc
      181 gcgataggcc gccagccgcg ccgccagcca gggcgcgaca atctcgcgcg gggcggccag
      241 cgtgaacaca tgggcagact ggcccgcctg catcgcctgc accgattcct cgaactgcag
      301 gaagccggcg cgcaagggtt ccaggcccgc cgaggcctcg gcggtcagct ccagcccctt
      361 ggccgtgcgg cggaacaata ccacgcccag caggtcttcc agcgcgcgga tctgctggcc
      421 aacggcggcc ggcgtcaccg ccagctcgtc ggcggcgcga gtgaaggaga ggtggcgcgc
      481 ggcagcgtcg aacacgcgca gggcattcag cggcagatgg gtccgtttca tgcgcccgcg
      541 ttagcgcaga atgccgcgtt cagccagcgg ttgcgggggc ggccagcgga aagaagggga
      601 tcgccacgcg caggtcaatc tcctcgcccg gagcggcatc ggtgaggtgg aaggtgtaat
      661 gcccctccat gctgccattg ggcgtggcca gcgggcagcc cgaaacataa tcatggcttt
      721 ggccgggacg cagcaccggc tgctcgccca ccacgccctc gccatccacc aggctgaccg
      781 cgccatggcc atcggtgatt cgccagtggc gggtgaccag ctgcatggtg cgggcggaat
      841 cattctcgat ccggatgtga tacacccaga accatttgcc cgcctcgata cgcgattgct
      901 cgggcaggaa attcaccgcc acgcgcacgg tgacgccagc agtggtcgcg gcatgttgga
      961 acagttcctt catggccgca atggtagcct gtggccggcc gcgagacaac agcgcttggc
     1021 gccgggcggc gggcggcctg gcgcaaaagc ggcctgctcg aaaatgctat tgcgagcgat
     1081 tatcatttat gccatccctt gggcctgttc aggagcccgc acatgcctca tccctccgcc
     1141 agacaccccc tctccccggc cgagacgccc gatcccctgg ccgcctgcct gccactgccc
     1201 atgcccgcgc atgaggatgc ccgcatgttg atgatcgccg cccggcgcat ggccgtgcac
     1261 gggctgcatg atgccagcgc cgcgctgctg gtgctgcgcc atctgggcat ccatttccgc
     1321 cagccgctgg tgctgatgcg ggcgtttatg ctggaactgt cggatgccac gcgccggccg
     1381 atcatggtgg cgccctgctg cacgccccgc ctcacccgcg atgaaggcga tatccttgcc
     1441 acctggagcg cggccagcca cgatccggcc gccgccatgg cgcatctggg ccggctgaca
     1501 gggggcccgg cgcccgccgc cctgctgacg acaggcgccg cccttgccaa tgcgctggcc
     1561 aatgccgggc gcgccctgcc gccatggccg ggcgcccctg ccgattgaac gggcgcttac
     1621 agcccgatgg accgcagcgc ctgatcgaga tcctcggtga ggtcagccac atcctccagc
     1681 cccacgctga tccgcagcat gccctcgctc acgcccatgg cggcgcgggt ttccggggtg
     1741 aggctggaat gggtggtgct ggcaggatgc gtcatcaggc tcttggtgtc gccgatattg
     1801 ttcgaaatat cgatgagttc cagcgcgttg agcaccgcca tcgcagcctc gcggccacct
     1861 gccacctcga aggagaagat cgagccggcc gccttcatct ggcgcatcgc cagcgcgtgc
     1921 tgcgggtggc tgggcaggcc cggatagagc aggctgggca ctcgcccttc cagaaagcgc
     1981 gccacttcgc ccgctgccgc ggcctgggca ttggccctga ggtccagcgt ttccagcccc
     2041 ttcagcacga tccacgcgtt gaacggcgag agcgtgttgc cagtgttgcg ctggaagggc
     2101 agcagcttgt tgttgatgaa atcggccgtg ccggccacag cgccagccag cacgcggccc
     2161 tgcccgtcca tcagcttggt ggcggcatag gccaccacat ccgcgccatg atcgagcggg
     2221 cgctggaggg cggaagaggc aaaggcattg tccaccacca cggtgatgcc atgttcatgc
     2281 gccagatcgg aaacggcgcg gatatccacg atatccatcg tcggattggc gggcgtttcg
     2341 aagaagaaca ccttggtgtt ggggcggatg gcggccttcc actggtcaag gtcgcgcccg
     2401 tccaccggcg ttgccgcaat gccgaagcgc ggcagcagat tgtccgtcac ccagcgggtg
     2461 gggccgaagg cggcgcgggc gatcaccata tggtcgccgg ccgaaagctg gcacagcagc
     2521 acgctcgaca tcgccgccat gccgctggcc tgggcgcggc aggcctcggc gccttccagc
     2581 agggcgatgc gctcttccag catctgcacc gaggggttct gcgtgcggct gtaggtcatg
     2641 ccgggggctt ggccggcaaa acgggccgcg ggcgtctctg catcctcgta gcaatagccc
     2701 gagttcatga agatggcttc gctggtctcg ccctgctcgc tgcgccaggt gccgccgcgc
     2761 accgcgcgcg tggcctgacg ccatttggaa gtgatggtgc gatcctgtcc ggtggtgcgt
     2821 ttcatgggcg gttctctagg cctcccggcg ccgaagggca agggcgtggc gggctctgca
     2881 ggggcattgc gatacccccg ccggctgcta tcgtggcgga tgatgaaggc ctttctcgca
     2941 agatcgcccg cgcgccagcg cgatccgctg ggctggtgcg ccgcgcttgc ccttgccttt
     3001 gcggcgctgg ccggatggcg gctgggcacg cccggccgga tctatttcga cgaggtgcat
     3061 tatgtgcccg ccgcccgcgc gctgctggcc ttgatcccgg ccaatccgga acatcccctg
     3121 tttgccaagc tggccattgc cgcggccatt gccacgctgg gcgatcagcc ggcggtgtgg
     3181 cgcctgccct cgctggtggc cggcgccatc ggcctgttcg cctttggccg ggcactgtgg
     3241 tgggccagcg ggcggcgctt tgccacgatt gccggcatgg tgctgctggc cagcgatttc
     3301 gcctggttca tccagagccg catcgccatg ctggatatgg tgatggccgg ccttgggctg
     3361 gtggcgctgg ggctggtggc gggcgccatc ggcctgccgc agggggcgcc ggcatggcag
     3421 cggcgctggc ggctggccgg cgccgggctg gcgctgggcc tggccatggg cgccaaatgg
     3481 agcgtgctgc cgctatgcgc ggggctggtg ctggcggtgc tggccgcgcg cgcgctggcg
     3541 atctggcgca tggggggcga aaccgccgcc accctgccga tccccggcat ttcccttgcc
     3601 gagctggcgc tgtggcttat ctcgctgccg ctggtggctt atgcggccac tttcacgccg
     3661 gccttcttct atcacagcca ggcgctggcg cccaccatcc ccgggctggt ggcctggcac
     3721 cgctatatgg tggcgctgca ggccagcgtc atcaagccgc atccctatca gagccactgg
     3781 tggcaatggg tgatcgactg gcgggcgatc tggtatctct acgagccggt ggatggcatc
     3841 cagcgcggca tcgtcctcat cggcaatccg gtgtcgatgc tggcggggct gccggcgctg
     3901 gtgtggtgcc tgtgggcggg ctggcgcggg cggcggctgg atgcggcagc ggcggtgggt
     3961 ttctatgcgc taaccctcgg cctgtgggtg gtggcgcaca agccggtgca gttctactat
     4021 cactacctgc tgcccggcac cttcctgatg gcagcgctag ccctggcgct cgacgatatc
     4081 tggcaggcgg gcgggccgct gcgctgggcc gcgcccgcgc tggtggcagc ggcggtggcg
     4141 gtgttcgcct ggttctaccc gattatcgcg gcgatgccgc tgcaccacgg cgtgatgagc
     4201 tttgaagact ggatgtggct ggacagctgg cgctgaaacg caaaacggcc ccggcccctg
     4261 cccgcccggt ggcgcggcag gggccggggc cgccgcttat gtcagttgcc gtatttgccc
     4321 cagacgaatt tggagctgag ggcaaagttc cacaccgagc ccagcacgat accgaccagc
     4381 accgccacca catcgtggaa atgcatgcgc ttgagggcag cggcggtggc cacattggtg
     4441 agcaggccga acgagcaggt gagcgcgaac ttgccccagc cgcgcagcac cggcaccagc
     4501 ccgcgcaggc gcttgtcgga ataggtcagc tcgttgttga gcacgaagtt gaaggtcatc
     4561 gccgccagcg ctgccaccgt gttgccgatg ttgaagcgca tgtcgttggt ccaggccgag
     4621 acgacgaagc gttcgcgcag caccatgtgc agcatcaccc acagcacgcc cagctgcacc
     4681 accacgccca gcgcccccac cgtgccgaac agggcaaagc gggtggggat gaagcggccc
     4741 agccacttgt cgtagaggcc aacgaggaac tcgaacacca ccgtgcggtc gagcttgctc
     4801 tcgccctcgg cacgggcggc aaaattcagg gggaattcct tcacgcgcag gggcctttcg
     4861 actgtggcaa gaatatcgag caggatcttg aagcccacgc ccgacaggcg gtgggcatct
     4921 gcacgcaggg tttcggcgcg cagcatgaaa tagccgctca tcggatcgga aagatcaacg
     4981 ccggtcacct tgcgggccag cgcattggca aagccagagg ccttcacccg gtcgggccgg
     5041 ccccattcct cggtgctggc gccttcggca aagcggctgg cataggctag gtcatacccg
     5101 ccgctctcga tcgcggccag catgccgggc agcaggttcg gatcgtgctg gtggtcggcg
     5161 tccatcaccg cgacgatggg ggcggcagtg gcgcacatcc cctcgatcgc cgcgctggca
     5221 aggccccgcc ggccaatccg ctgaatgcag cgcacccgcg gatcttccag attgatcgcc
     5281 cgcgcctcat cggccgtgcc atcccggctg ttgtcgtcca cgataatcgc ctcccagcga
     5341 atatcgccca gcgccttcgc cagcctctcg accagcgggc gcaaattctt gcg
//
LOCUS       DASRYE010000038         7622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_36453,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000038 DASRYE010000000
VERSION     DASRYE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36453"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFF98_02060"
     CDS             <1..472
                     /locus_tag="VFF98_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62441.1"
                     /translation="GGLDVLVNVAGGFVWQPLADDDGTSWPRMFALNALTCRNMCAAA
                     LPALRQAAAGRIVNIGAAGALQAGAGMGSYAASKAAVHKLTESLAAELAGTACTVNAV
                     LPTIIDTPANRADMPGADPGQWVQPQAIADAILFLASPQARAISGALLPLSRGG"
     gene            complement(478..1431)
                     /locus_tag="VFF98_02065"
     CDS             complement(478..1431)
                     /locus_tag="VFF98_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62442.1"
                     /translation="MRILRQQICPATASALPRLMDRLSALEAFVAVAETGSFTRAAAQ
                     RRLSTPMMTLHVARLEEHLGARLFNRTTRRVDLTEEGLRLLPHAREVIAAYGAAEGAL
                     LPGRGLVGRVRIDAPASIGHRRIVPLLAELQAAYPAIAIDLSLGDRGTVFRIDGFDII
                     LRVGEAPLSGWITKPLGETRQLCIAAPAYLARHGTPARPEDVHAHRCLLYASVEAPGG
                     SPWLFARNGRPIRLRPPPAHTFNDGAAILAAARAGLGIAQHLETIARDDLLAGALVPV
                     LLDHVAPAITVSLMSARERHGLPHVRAIMDYLIERVDWQLD"
     gene            1612..2766
                     /locus_tag="VFF98_02070"
     CDS             1612..2766
                     /locus_tag="VFF98_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU62443.1"
                     /translation="MAARLRVGIISANWSLRVHGAAWRRIEGVEVAAICTSRQETAER
                     AAQAFAIPKAYWNHEDMAADPDLDIIDVGTRPSFRFPMVMAALRHGKHVYDALPFAMS
                     LADAEAMRDAQQAAGVVGVVDAQFRWSPPAQQMKAMVEAGFIGRPLGFNVQLMLPLVE
                     HEGKPYAYSAFSEHTDPYYWLAEKSSGGSAWRNFATHALLFLTHLLGPVDEATGMLTT
                     GVPEWFLPNGEVLRPETHDLGSATLRLANGAIGSLQAGWCVPDTEGWRVEIWGDRGRL
                     LLVDNSFGNLQSARLYAGDARLLTYGARSCAPVPIDPAFYAVPGTTTGESGPRDFVAM
                     DWMFSRMADAIRHGTPASPSFAEAAAVQRVVEAVERADAERRWIRMAEMG"
     gene            complement(2801..3571)
                     /locus_tag="VFF98_02075"
     CDS             complement(2801..3571)
                     /locus_tag="VFF98_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62444.1"
                     /translation="MTDSRRRFAGRSVIVTGAGSGIGEAAAHAFAAEGARVALAELDA
                     ARGEAVAQAIRAAGGTALFVPTDATDEASVAAMVAAACAAHGPVRHAFNNVGAARPGS
                     LEGQSLEDWNWTMSISLTSTFLAMKHEIPVMKAHGGGTIVNTASMSGRIFTPSAPPAY
                     AAAKAGVIHLSHYASCAHAADDIRVNAVLPGLTLTPQIEAFFTPEQRRAVAAEHQVIH
                     RCVLPAEIAAAVLFLSSDEAAMITGRGIEVAGGGGHPG"
     gene            complement(3564..4133)
                     /locus_tag="VFF98_02080"
     CDS             complement(3564..4133)
                     /locus_tag="VFF98_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62445.1"
                     /translation="MNADLLARIDRLESQAAIQQLPIRYALAVDGRDIAGWTQLFVPD
                     VDCGRHGRGRAVLAGLIGPALRSFYRSVHQICGHQLAFDDADHAHGIVYCRAEHEEGG
                     KWVVMAIAYFDTYARREGEWFFLRRREKHWYAADWQERPAAPFTGWPAHPAPPGLPQQ
                     FATWADFWAGEDEGLIARLTSQPTGETND"
     gene            complement(4130..5272)
                     /locus_tag="VFF98_02085"
     CDS             complement(4130..5272)
                     /locus_tag="VFF98_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZU62446.1"
                     /translation="MGLIDPLLQPLALRGLVLPNRVVMAPMTRYRSPGGVPTAEVADY
                     YARRAATGLGLILTEGVGLGHYAAVDHPDIPRIASAEQQAGWRCVCDAVHGAGGRIIP
                     QLWHQGPMWNVEYAGAGTGTPMRPSGIWGPADGVISFRPEGRAQSLAPVPAMSESDIA
                     EVIAAYARAARDAVALGADGIAIHAAHGYLIDSFLWDYTNRRDDRWGGDPARRAAFGA
                     AVVRAIRAEVGEARPIVLRFSQFKMQDYRAVLADTPAGLEALLGPLADAGVDLFDASQ
                     RYFDTPTFAGSALNLAGWAKRLTGKASMTVGGVALDKASGSARHVDDSQPSVNNLPLL
                     LERFGRGEFDLVAVGRAILNDPAWFARAVADAPFLPFDPANLERLA"
     gene            complement(5283..6086)
                     /locus_tag="VFF98_02090"
     CDS             complement(5283..6086)
                     /locus_tag="VFF98_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU62447.1"
                     /translation="MGDIVRDEPAEGVCRLTLNRPEALNALTFAMYAELIDHLEAVRY
                     DHDVRVVLLTGAGRGFCAGHDIRAGGTPGWVPEGLGKAQAQRRVMDLLGEIPQRLHAL
                     PQPVICAMNGVAAGAGYALALAADMTLAARSAKFVNAFHNAGSGHEMGFSYLVPRLVG
                     MQRAAELMLTGRAIPAEEAAAMGLVLRCVEDAALMDEALALAEAVMANSPIGIALTKS
                     SMWANVDAPGLAAAIELENRAIFMSQSTADTAEKRQAFLEKRRPRFTGA"
     gene            6276..7505
                     /locus_tag="VFF98_02095"
     CDS             6276..7505
                     /locus_tag="VFF98_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZU62448.1"
                     /translation="MARLPLDTIRILDLTRVLAGPFGSQMLADMGADVIKIERPGAGD
                     DARIFGEPYLRDAQGQPSRENAFYLSVNRNKRSVALNIAHPKGQALIRELVKSCDVVM
                     ENYKVGDLARYGLDYESLKAINPGLIYCSVTGYGQSGPLAGKPGYDAVFQGECGLMSV
                     TGVPDGKPGAGPMKVGPSIIDLFTGLNVANAILAALYHRDAQGGEGQHIDIALLDCGV
                     AALSHYAQIYLTSGEVPVRRGTQGNGGMPTSMFACSDGAIMITSGNDRQYVALCAAVG
                     HPELASHPRFATNVLRVAHRDEITEVFNAIFATNSVAHWLEKLGAAGIPSGPINDLAE
                     VFAYPQAVHRGLRARAPHPLKPDLDVIRSPLHFSATPITDYRAPPMLGEHTRTLLAEE
                     LGLDEAALDALRDEGII"
BASE COUNT         1208 a         2707 c         2594 g         1113 t
ORIGIN      
        1 aggcgggctg gatgtgctgg tgaatgtggc cggcggtttc gtgtggcagc cgctggccga
       61 tgatgatggc accagctggc cgcgcatgtt cgccctgaac gcgctgacct gccgcaacat
      121 gtgcgccgcc gccctgccgg cgctgcgcca ggcggcggcg ggccgcatcg tcaacatcgg
      181 cgcggcaggc gcgctgcagg cgggcgccgg catgggcagc tatgccgctt ccaaggccgc
      241 cgtccacaag ctgaccgaga gcctggcggc tgaactggcc ggcaccgcct gcacggtgaa
      301 cgcggtgctg cccaccatca tcgacacacc ggccaaccgc gccgacatgc ccggcgccga
      361 ccccggccaa tgggtgcagc cgcaggcgat cgccgatgcg atcctgttcc tcgcctcgcc
      421 gcaggcccgc gcgatcagcg gggcattgct gccgctgtcg cgcgggggct gaaagcctca
      481 gtccagctgc cagtccaccc gctcgatcag gtaatccatg atcgcgcgca catgcggcag
      541 gccatggcgc tcgcgcgcgc tcatcagcga gacggtgatc gccggcgcca catgatcaag
      601 cagcaccggc accagcgcgc cggccagcag atcatcgcgg gcgatggttt ccagatgctg
      661 ggcaatgccc agccccgcgc gcgccgccgc cagaatggcc gcgccatcgt tgaacgtgtg
      721 ggccggcggc gggcgcaggc ggatcggccg gccattgcgg gcaaacagcc aggggctgcc
      781 accgggcgcc tccacgctgg catagagcag gcagcgatgg gcatgcacat cctcgggccg
      841 ggcgggggtg ccgtggcggg ccagataggc gggcgcggcg atgcacagct ggcgggtttc
      901 gcccagcggc ttcgttatcc agcccgacag cggcgcctcg cccacgcgca ggatgatgtc
      961 gaacccgtcg atgcggaaca ccgtgcccct gtcgcccagg ctgagatcga tggcaatggc
     1021 gggataggcc gcctgcaatt cggccagcag cggcacgatg cggcgatggc cgatcgaggc
     1081 cggcgcatcg atccgcaccc ggcccaccag cccccgcccc ggcagcagcg cgccttcggc
     1141 cgcgccatag gcggcgatca cctcgcgcgc atggggcagc aggcgcaggc cctcctcggt
     1201 caggtccacc cggcgggtgg tgcggttgaa caggcgcgcg cccagatgct cctccagccg
     1261 ggcgacatgc agcgtcatca tcggggtgga caggcgacgc tgcgccgccg cccgggtgaa
     1321 cgagccggtt tcggccaccg ccacaaaggc ttcaagggcg ctcagccggt ccatcaggcg
     1381 cgggagggcg ctggccgtgg cggggcagat ctgctggcgg aggattcgca aggggccatg
     1441 ggccaagcat tggcgcattg gcgcatcagg tcaaacccgg cgccatcatg cctgtgatga
     1501 gatgccccgc cggcccgatc cgcattgcct gcgccggccg gcagggccca tttgccatcc
     1561 ccatggcacc ggcccatcgg cggccggcaa caccatgggg gagacagggc aatggcagca
     1621 cggctgcgcg tgggcatcat cagcgccaac tggagcctca gggtgcatgg cgcggcctgg
     1681 cggcggatag agggggtgga agtggccgcc atctgcacct cgcgccagga aaccgccgaa
     1741 cgcgcggcgc aggcctttgc catccccaag gcctattgga accacgagga catggccgcc
     1801 gaccccgatc tcgacatcat cgatgtcggc acccggcctt cgttccgctt ccccatggtg
     1861 atggccgcgc tgcgccatgg caagcatgtc tatgatgcgc tgccctttgc catgtcgctg
     1921 gccgatgccg aggcgatgcg cgatgcccag caggccgccg gcgtggtcgg cgtggtcgat
     1981 gcgcagttcc gctggtcgcc gcctgcccag cagatgaagg cgatggtgga ggcaggcttc
     2041 atcggccggc cgctgggctt caacgtgcag ctgatgctgc cgctggtgga gcatgagggc
     2101 aaaccctatg cctacagcgc cttttccgaa cacaccgatc cctattactg gctggccgaa
     2161 aagagcagcg gcggcagcgc ctggcgcaat tttgccaccc atgcgctgct gttcctcacc
     2221 catctgctgg gcccggtgga cgaggccacc ggcatgctga ccactggcgt gcccgaatgg
     2281 ttcctgccca atggcgaggt gctgcgcccg gaaacccacg atctgggcag cgccacgctg
     2341 cgcctggcca atggcgccat cggcagcctg caggcgggct ggtgcgtgcc cgataccgaa
     2401 ggctggcggg tggagatctg gggcgacagg ggccgcctgc tgctggtgga caacagcttc
     2461 ggcaacctgc aatcggccag gctgtatgcg ggcgatgcgc ggctgctcac ctatggcgcg
     2521 cgcagctgtg cgccagtgcc gatcgatccg gccttctatg ccgtgcccgg caccaccacc
     2581 ggcgagagcg gcccgcgcga tttcgtggcg atggactgga tgttcagccg catggccgat
     2641 gccatccgcc atggcacgcc cgcctcgccc agctttgccg aggcggccgc cgtgcagcgc
     2701 gtggtggaag cggtggagcg cgccgatgcc gaaaggcgct ggatccgcat ggccgagatg
     2761 ggctgaggcg cccctctcgc gcgcccgccg ggcggcggct ctagcccgga tgcccgccgc
     2821 cgccggccac ctcgatgccg cgcccggtga tcatcgccgc ctcgtcgctg gataggaaaa
     2881 gcaccgccgc ggcgatctcg gccggcagca cgcagcggtg gatcacctga tgttcggccg
     2941 ccaccgcgcg gcgctgctcg ggcgtgaaga aggcctcgat ctggggcgtg agggtgaggc
     3001 cgggcagcac cgcattcacc cggatatcgt ctgccgcatg ggcgcagctg gcataatggc
     3061 tgagatggat cacccccgcc ttggccgccg cataggccgg cggcgccgag ggcgtgaaga
     3121 tccgccccga catcgaggcg gtgttgacga tggtgccgcc gccatgggcc ttcatcaccg
     3181 ggatctcgtg cttcatcgcc agaaaggtgc tggtgagcga gatgctcatg gtccagttcc
     3241 agtcctccag gctctgccct tccagcgagc cggggcgcgc ggcgccgaca ttgttgaagg
     3301 catggcgcac cggcccgtgg gcggcgcagg cggccgctac catcgctgcc acagaggcct
     3361 cgtcggtggc gtctgtcggc acgaacaggg cagtgccgcc cgccgcacgg atcgcctgcg
     3421 ccaccgcctc gccgcgggca gcatccagtt cggccagggc cacgcgcgcg ccctcggcgg
     3481 caaaggcatg cgccgctgcc tcgccaatgc ccgatccggc gccggtcaca atcacgctgc
     3541 ggcctgcgaa acggcggcgg ctgtcagtca ttcgtctctc ctgtcggttg gctggtgagg
     3601 cgggcgatca gcccttcatc ctcgccggcc cagaaatcgg cccatgtggc aaactgctgc
     3661 ggcaggccgg gcggggcggg atgcgccggc cagccggtga agggggcggc gggtcgctcc
     3721 tgccagtcag ccgcatacca gtgcttctcg cggcggcgca ggaagaacca ttccccctcg
     3781 cgccgggcat aggtgtcgaa ataggcgatc gccatcacca cccatttgcc gccctcctca
     3841 tgctcggccc ggcaatagac gatgccatgg gcatgatcgg catcatcgaa cgccagctgg
     3901 tggccgcaga tctggtgcac cgagcgatag aagctgcgca gcgccgggcc gatcagcccc
     3961 gccagcaccg cgcgcccgcg gccatggcgc ccgcaatcga catcgggcac gaacagctgc
     4021 gtccagccgg cgatatcgcg gccatccacc gccagcgcat agcggatcgg caattgctgg
     4081 atcgccgcct gcgattccag ccggtcgatc cgggcgagca gatccgcgtt catgccagcc
     4141 gctccagatt ggccgggtcg aagggcagga agggggcatc ggccaccgcc cgtgcaaacc
     4201 acgccggatc gttgaggatg gcccggccca ccgccactag atcgaactcg ccccggccaa
     4261 agcgttccag cagcagcggc aggttgttca ccgatggctg gctgtcgtcc acatggcggg
     4321 cgctgccgct cgccttgtcg agcgcgacgc cgcccaccgt catcgatgcc ttgccggtga
     4381 ggcgcttggc ccagccggcc aggttcagcg ccgatccggc aaaggtgggc gtgtcgaaat
     4441 agcgctggct ggcatcgaac agatccacgc cggcatcggc cagcggcccc agcaaggctt
     4501 ccagcccggc cggcgtatcg gccagcacgg cgcggtaatc ctgcatcttg aactgcgaga
     4561 agcgcagcac gatcggccgc gcctcgccca cctcggcgcg gatggcgcgc accaccgccg
     4621 cgccaaacgc tgcccgccgc gccggatcgc cgccccagcg atcgtcgcgc cggttggtat
     4681 agtcccagag gaaactgtcg atcagatagc catgggcggc gtggatggcg atgccatcgg
     4741 cccccagcgc caccgcatcg cgcgcggcgc gggcataggc ggcgatcacc tcggcaatgt
     4801 cgctctcgct catggccggc accggcgcca ggctttgcgc gcgcccttcc gggcggaagg
     4861 agatgacgcc atcggccggc ccccagatgc ccgaggggcg catgggcgtg ccagtgccgg
     4921 cgccggcata ttccacattc cacatcggcc cctgatgcca cagctggggg atgatgcgcc
     4981 cgcccgcgcc atgcaccgca tcgcagacac agcgccagcc ggcctgctgt tcggcgctgg
     5041 cgatgcgcgg gatatcggga tgatccaccg cggcataatg gcccagcccc accccttcgg
     5101 tcaggatcag gccaaggccg gtggcggcac ggcgggcgta ataatcggcc acttcggcgg
     5161 tgggcacgcc gccgggcgag cgatagcgcg tcatcggcgc catcaccacc cggttgggca
     5221 gcaccagccc gcgcagggcc agcggttgca gcagcggatc gatcaggccc atccccctct
     5281 cctcaggcgc cggtgaagcg cgggcggcgc ttttcaagga aggcctgccg cttctcggcg
     5341 gtatcggcgg tggactggct catgaagatc gcccggttct ccagctcgat cgccgccgcc
     5401 agccccggcg catcgacatt ggcccacatc gagctcttgg tcagggcgat gccgatcggg
     5461 ctgttggcca tcaccgcctc ggccaaggcc agcgcctcgt ccatcagcgc tgcatcctcc
     5521 acgcagcgca gcacaaggcc catggccgcg gcttcctcgg cggggatggc gcggcctgtc
     5581 agcatcaatt ctgccgcgcg ctgcatgccc accagccgcg gcaccagata gctgaagccc
     5641 atctcgtggc cgctgccggc attgtggaag gcgttgacga atttggccga acgggccgcc
     5701 agggtcatgt cggcggccag cgccagcgca tagccggcgc ctgcggccac cccgttcatc
     5761 gcgcagatca ccggctgcgg cagggcatgc aggcgctggg ggatctcgcc cagcaggtcc
     5821 atcacccggc gctgggcctg cgccttgccc agcccttcgg gcacccagcc gggcgtgccg
     5881 ccggcgcgaa tgtcgtggcc ggcgcaaaag ccgcgcccgg caccggtcaa cagtaccacg
     5941 cgcacgtcat gatcgtagcg caccgcttcc agatgatcga tcagctcggc atacatggca
     6001 aaggtcagcg cgttcagcgc ctcgggccgg ttcagcgtca accggcacac accctcggcc
     6061 ggctcgtcac gcacgatatc gcccatttgc gtcctcatcc ctggcaggcg ggccctccat
     6121 ggccccgttg ccgccattgt gggtgcattg cccgcccggt ggaacaggca tcgcccgcct
     6181 gccgcgccat atcacaattg tgaacaggcc ggcgcccggc ttgccatcgc gcagggccca
     6241 caggaagcag gagcgcagcc acaacaggac gatccatggc cagactaccg ctcgacacga
     6301 tccgcatcct cgacctcacc cgcgtgctgg ccggcccctt cggatcgcag atgctggccg
     6361 acatgggcgc cgatgtaatc aagatcgagc ggccgggcgc cggcgatgac gcgcgcatct
     6421 tcggcgagcc ctatctgcgc gatgcccagg gccagcccag ccgcgaaaac gccttctacc
     6481 tctcggtcaa ccgcaacaag cgctcggtgg cgctgaacat cgcccatccc aagggccagg
     6541 cgctgatccg cgaactggtg aaaagctgcg atgtggtgat ggaaaactac aaggtgggcg
     6601 atctggcccg ctatggcctc gattacgaga gcctgaaggc gatcaacccc gggctgatct
     6661 attgctctgt caccggctat ggccagagcg ggccgctggc cggcaagccc ggctatgacg
     6721 cggtgttcca gggcgaatgc gggctgatga gcgtgaccgg cgtgcccgat ggcaagcccg
     6781 gcgccgggcc gatgaaagtg ggccccagca tcatcgacct gttcaccggg ctgaatgtgg
     6841 ccaacgccat cctggccgcg ctctatcacc gcgatgccca gggcggtgaa gggcagcata
     6901 tcgatatcgc cctgctggat tgcggggtgg cggcgctgtc gcattatgcg cagatctatc
     6961 tcacctcggg cgaggtgccg gtgcgccgcg gcacgcaggg caatggcggc atgcccacca
     7021 gcatgttcgc ctgcagcgat ggcgcgatca tgatcacctc gggcaacgac cggcaatatg
     7081 tggcgctgtg cgcggccgtt ggccatccgg agcttgccag ccatccgcgc tttgccacca
     7141 atgtgctgcg cgtggcccac cgcgacgaga tcaccgaggt gttcaacgcc atctttgcca
     7201 ccaacagcgt ggcccactgg ctggagaagc tgggcgcggc cggcattccc agcgggccga
     7261 tcaacgatct ggccgaggtt ttcgcctatc cgcaagcggt gcaccggggg ctgcgggcgc
     7321 gggcgcccca tccgctcaag cccgatctcg atgtgatccg cagcccgctg cacttctcgg
     7381 ccacgccgat caccgattat cgcgcgccgc ccatgctggg cgagcacacc cgcacgctgc
     7441 tggccgagga actggggctg gacgaggccg ccctcgatgc cctgcgcgat gagggcatca
     7501 tctaggcggc gacgcctcag gccgggcgcc ccgtcaccag cctgggcacc gccgcgaaga
     7561 aggcgcgccc gcctgcctcc agccggatga attcgctgaa atggccgagg tcgcggcggg
     7621 tg
//
LOCUS       DASRYE010000039        11892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_214328,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000039 DASRYE010000000
VERSION     DASRYE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214328"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VFF98_02100"
     CDS             complement(<1..1171)
                     /locus_tag="VFF98_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU62449.1"
                     /translation="MATRLVRLASTLVIARRLDAGIVGEAALALSLFELIRVLAQVGI
                     GQAIIAAPADALAATCNTARRLFTLWAALLTALQLAVAGGLALAGGPDWQKSAEMLAV
                     LALVYPFTPAGQVQIALAQREGLAGPVSRCAATQTITSNLLSTALLLAWRSPWALVLP
                     RLLTAPLWLAMARRLRPWQALPAAGQAPAAGLLRFGLAVLVAEIWAGLRTQGDNLLIA
                     ALLGARALGTYYFAFNAGVGIVSSLVSAFGMVVFPALCAAPRGAPRAAALERVLIAGL
                     GLFLPLVALQSLAARWYVPIVFGGHWAYAAPLVGILCLSGAPQLVAAITSLWLRAEGR
                     LRQDMAGSALSCVAALGLLALAAALAPPATGLAWAAMGLVAGQGLAALAYHLPILR"
     gene            complement(1441..2199)
                     /locus_tag="VFF98_02105"
     CDS             complement(1441..2199)
                     /locus_tag="VFF98_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62450.1"
                     /translation="MNQSPIAIITGGSRGLGRASVEALARRGVSSIFTYHSNRAAAQA
                     VVAAAEQAGARAAALPLDTGDTAALPGFVEAVEAQLAAWGAARFDYLVNNAGTAHHAA
                     IADVTEADFDALYQVHVKGVFFLTQRLLPLIADGGRIVNISSGLTRFSFPGSCAYAAM
                     KGAVEVMTRYLARELGARSIAVNTVAPGAIETDFSGGRVRDDPAVNRFVAEATALGRV
                     GLPDDIGPMIAALLAPENRWINAQRIEVSGGMVV"
     gene            2303..3199
                     /locus_tag="VFF98_02110"
     CDS             2303..3199
                     /locus_tag="VFF98_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62451.1"
                     /translation="MDRLATLELFTRIVERGSFRAAAASLGVSRPVATAAIKALEQRL
                     GARLLQRTTRHVRPTAEGEAYYRRCLAILADLEAAERDVNGAVAGVVRVDVAGRLARG
                     LLLPALPAFLARHPALTVYLGEGERFVDLVREGVDCVVRAGPLADSDMIARQLGVMEE
                     ITCASPAYLAAHGVPRSPDDLAGHSMIGFVSSRTGHPLPLEFAVAGQMVEVALPARLL
                     VNGAESSAAAARLGLGLMQAPRHGLAGDLAAGALVEVLAEYRPAPTPVALLYPSNRQL
                     SARVRVFADWLVAILAPALAAG"
     gene            complement(3209..4786)
                     /locus_tag="VFF98_02115"
     CDS             complement(3209..4786)
                     /locus_tag="VFF98_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62452.1"
                     /translation="MNVLLQQAIGGQRRFVRLGILCAGIAALAAVGLLALSGWFLAGA
                     ALAGMGGVLAVQAFNYLLPSAAIRAAAILRTGTRYGERMLGHRAALFALAELRMALFG
                     RVAARALADANIGRSGAMANRLGKEVDALEDAVIRQISRPGAWVAAGAGLAAAAILGW
                     PALLVLGLAMAAMRLAGRQMAARLLPAPLAQAAAAHAALQADYADLAGPCADIAVYGL
                     APAMAAALAETARAQDAARRALARAEGLILAGQTAMAALATAALAAVATAPAPLCALG
                     LLGAMAALEGWAALAQTDMRGHQLALAEAHLAAISQAPDPAPMATAASAPALRLCGQQ
                     VAPDARVQLCGPSGAGKTRLAETLVGLRRDAPQDIAVAGSDPRALGLAALRPCFALAA
                     QEAPLMAGSVADNLALARPGLSEAQMWEALHIAVADEVVRALPDGLHQWLGGDGARLS
                     GGQRRRIALARALLAGRPWLVLDEPSEGLDAATEALLAARLEGWLDRTGTGLVLISHR
                     PAMGRLARACIDVAPAG"
     gene            complement(4783..6390)
                     /locus_tag="VFF98_02120"
     CDS             complement(4783..6390)
                     /locus_tag="VFF98_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62453.1"
                     /translation="MSSADTPSLPPTKVDRRAAFWWLVDSAGAVPVAAGVALVVAARL
                     PGAGSAPGPALALLAGGGLLRAAALWRAQVAGAGAAARLQAALRRVLHPALLPSRLMR
                     GRLIGEDMHIAIDAIAATEGLAARFLPLRLASGATPLLIALAVAPASWVASLILLATL
                     VPFILAMALAGGAAATRAQAQHQALARLSGLFVDRLRALPMVLSFAAEPRVTRHLGAA
                     AQEVARRTMAVLAIAFASSAILEFFAALAVALVAVYCGFSLLGLLPFPAPEHLSLWRA
                     FYALALAPEFYLAMRRLAAAYHDKQQGEAAIAAMAAQLAASAPPPPPIAAPLRWQGRA
                     VVLRHGDGTTIGPLDWDWQGPGLHLLTGPTGAGKSSLLLALIGQVPLGAGAIRADGHA
                     FAPGALNHAIGWAGQQVALLPGTLRANLAPGAAHPPSDGAMRPCLEALGLGAMLRARG
                     GLEMAIDHRGSSLSGGERRRIGLARAVLSGRPLLLLDEPTADLDAATARAVRALLAEL
                     ARSRLVIAASHDAALIGMAASVCEVAP"
     gene            6509..8074
                     /locus_tag="VFF98_02125"
     CDS             6509..8074
                     /locus_tag="VFF98_02125"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZU62454.1"
                     /translation="MDMAVIELSRLQFALTAMYHFLFVPLTLGLSFMLVIMESVYVIT
                     KRPIWRVITRFWGRLFGINFVLGVATGLTMEFEFGTNWAYYAHYVGDIFGAPLAIEGL
                     MAFFLEATMVGVMFFGWDRLSRRQHLLVTFLTALGTNLSALWILVANGWMQNPVGASF
                     NPATMRMEVTDFKEVLFNPVAQAKFVHTVSAGYVCAATFVMGISALYLLRGKYTNVAR
                     RSMMVASAFGLASALSVVVLGDESGYALTDNQKMKLAAIEAMWETEPAPAAITLVGLP
                     DIAQRTTHHAVKLPYVMGLIGTRSLSGKIDGIEELVLRADGRIHSGLIAYDAVEKLKQ
                     NRADTAAMAAFEGHKRDIGFALLLKRYVADPRQADDRLILKAAWDTVPNVPVMFFLFR
                     FMAGLGFAFIGFFALAFYCASACRFRKRWFLKLAVCMLPLPWVAIEFGWVLAEIGRQP
                     WAVDGVLPTFLGASSLSVPQVWATIFGFTALYGTLAVIEVRLMIAAIRKGPIEHHEDD
                     GQAEAGFAPLPAE"
     gene            8088..9236
                     /gene="cydB"
                     /locus_tag="VFF98_02130"
     CDS             8088..9236
                     /gene="cydB"
                     /locus_tag="VFF98_02130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZU62455.1"
                     /translation="MHIPLDYETLRLIWWVLLGVLLIGFALTDGYDLGVASLLPFVAR
                     TDEERRQAINAIAPHWEGHQVWLILGGGAIFAAWPFVYAVSFSGFYLAMFLVLAALIL
                     RPVSFKYRSKHADAGWRARWDWALFTGGFVPALVFGVAVGNVLAGAPFRFDSDLRMTY
                     EGTLLGLFTPFTVLAGLLSVAMLAMHGAAWLAVKVADGPVVARARRFGLWAAAASVAL
                     FALGGWMVAHGGMGMRLVGVADAGGPSNPLLTASTFAPGAWLDNFAAHPWMVAAPVAG
                     LLAPLLAMAGIAGRLGWLQLAGSALSITGIIATVGCAMFPFILPSSIDPRSSLTVWNA
                     SSSHLTLFIMLVCTVVLLPMVLAYTAWVMKLLAGRITLAEVRSNPDFY"
     gene            9249..9359
                     /gene="cydX"
                     /locus_tag="VFF98_02135"
     CDS             9249..9359
                     /gene="cydX"
                     /locus_tag="VFF98_02135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HZU62456.1"
                     /translation="MWYFSWILGLGLAVGFGIINGLWHEFNSPIDDDPAI"
     gene            9576..9893
                     /locus_tag="VFF98_02140"
     CDS             9576..9893
                     /locus_tag="VFF98_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU62457.1"
                     /translation="MKPADLTELKALAAHMAGRLKVMSHPERLLMLCRMDEGEVSVNE
                     LVALTGLSQSAVSQHLAMLRAEEVVGVRIDANIRWYRLTDPIVSGIIHALCALCEPQA
                     AQP"
     gene            complement(9922..9997)
                     /locus_tag="VFF98_02145"
     tRNA            complement(9922..9997)
                     /locus_tag="VFF98_02145"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9962..9964),aa:Ile,seq:cat)
     gene            complement(10057..11043)
                     /locus_tag="VFF98_02150"
     CDS             complement(10057..11043)
                     /locus_tag="VFF98_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU62458.1"
                     /translation="MSAPAPGSDVRFSGGPGPRFLLTVDTEEEFDWSRPLERGGHSTD
                     TVARLRKFQEFCEGQGVVPIYLIDYPIAISELAADLLRAPLAAGRAEIGVQLHPWVNP
                     PFEEQVTQFNSFAGNLPYELERAKLMSLRDTIERNFGQTPRIYRAGRYGLGPNTAQIL
                     REAGIAIDTSVRSRFDYSSHGGPNYATRPLTPWWVDEGRTLMELPLTTLFTGHLRRRA
                     QWLYPRLWRLPQMRSVLARLGMMDRIPLTPEGVTIAEALRGVRQAVAEQLPVIVLSFH
                     SPSLSPGHTPYVRSEDDLDKLYDWWRAVFAELRALGACPSSVADIMGSVELA"
     gene            complement(11102..>11892)
                     /locus_tag="VFF98_02155"
     CDS             complement(11102..>11892)
                     /locus_tag="VFF98_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZU62459.1"
                     /translation="PAAGTAPPPPRETGSDRLRQLLHELRTPVNAVQGFAEIIQQQLF
                     GPAPHEYRALAAHIAADSARMLAGFEELERFAKLDSGAMALPAGSGDLFACVAATIAR
                     LEPHSTPRASGFQLGAPPAPEADGTPPLAVALAPAELERLVWRLLATLAANAGPGEVL
                     PVELVATGGYKVLAMALPRTLAGLDDHAFYHAQGSTGPEAPFAGMFGTGFALRLASAE
                     ARAAGGDLLRQGAALQLRLPGLTAAAAPPSHPARGQAVGAAPAA"
BASE COUNT         1782 a         4229 c         4123 g         1758 t
ORIGIN      
        1 ggcgcaggat cggcaggtga taggccagcg ccgccagccc ctggcccgcc accagcccca
       61 tggcggccca ggccagccct gtggccggcg gtgccagcgc cgctgccagc gccagcagcc
      121 ccagcgccgc cacgcaagac agcgcgctgc ccgccatatc ctgccgcagc cgcccttcgg
      181 cgcgcagcca caggctggtg atcgccgcca ccagctgcgg cgctccgctg aggcacaata
      241 tgcccaccag cggcgcggca taggcccagt gcccgccgaa aacgatgggc acataccagc
      301 gcgccgccag cgattgcagt gccaccagcg gcaggaacag gccaaggccg gcgatcagca
      361 cccgctccag cgcggcggcg cggggagcgc cgcgcggcgc cgcgcacagc gccggaaaca
      421 ccaccatgcc aaaggccgat accagcgagg agacgatgcc caccccggcg ttgaacgcga
      481 aataataggt gcccagcgcc cgcgcgccca gcagcgcggc gatcagcaga ttatccccct
      541 gggtgcgcag cccggcccag atttccgcca ccagcaccgc caggccaaag cgcagcagcc
      601 ctgccgccgg ggcctggccg gcggccggca gcgcttgcca ggggcgcagg cggcgggcca
      661 tcgccagcca cagcggcgct gtcaacaggc ggggcagcac cagcgcccag gggctgcgcc
      721 aggccagcag cagcgcggtg gacaggaggt tgctggtgat cgtctgcgtg gcggcgcagc
      781 gtgagacagg gcccgccagc ccctcgcgct gggccagcgc gatctgcacc tggccggccg
      841 gcgtgaaggg atagaccagc gccagcaccg ccagcatttc ggcgcttttc tgccagtccg
      901 gcccgcccgc cagcgccagc ccgcccgcca ccgccagctg cagcgcggtg agcagcgcgg
      961 cccacagggt gaacaggcgc cgggcggtgt tgcaggtggc ggccagcgca tcggccggcg
     1021 cggcgatgat cgcctggcca atgcccactt gcgccagcac ccggatcagc tcgaacaggc
     1081 tgagcgccag cgccgcctcg cccacgatgc cggcatccag ccgccgggcg atcaccagcg
     1141 tgctggccag ccgcaccagc cgcgtggcca tttccgcccc gccccagcga ccgaaatcgc
     1201 acagcagggg ccgcaacccc tgaagccgca ctggaagacc gcgagaaagc atgccgcacc
     1261 ggagtgttat tgcgaatgct tatcggatta acctgtctgg tattaataaa atcctcaaaa
     1321 taaataaatt aaatcagcat ggtgcggtta attccggcgg gctgtccacg gctgcccgaa
     1381 ggcggtggga gagaaggcgg cggcgccttt ccatggttaa ccgccgcgct tccctcacgt
     1441 tcacaccacc atgccgccag acacctcgat gcgctgggcg ttgatccagc ggttctcggg
     1501 cgccagcagg gcggcgatca tcgggccgat gtcgtcgggc aggcccaccc ggcccagcgc
     1561 cgttgcttcg gccacaaagc ggttcacggc cggatcgtcg cgcacgcggc cgccgctgaa
     1621 gtcggtctcg atcgcgccgg gcgccacggt gttgacggca atgctgcgcg cgcccagctc
     1681 gcgcgccaga tagcgcgtca tcacttccac cgcgcccttc atcgccgcat aggcgcagct
     1741 gcccggaaag ctgaaccgtg tgaggccgga ggagatgttc acgatccgcc cgccatcggc
     1801 gatcagcggc agcaggcgct gggtgaggaa gaacacgccc ttcacatgca cctgataaag
     1861 cgcgtcgaaa tcggcctcgg tcacatcggc gatggcggcg tgatgggcgg tgccggcatt
     1921 gttgacgaga tagtcgaagc gggcggcgcc ccaggcggcc agctgcgctt ccacggcctc
     1981 aacgaagccg ggcagggcgg cggtgtcgcc ggtgtcgagc ggcagggcgg cggcccgggc
     2041 gccggcctgt tcggcggcgg ccaccaccgc ctgggccgcg gcgcggttgc tgtgataggt
     2101 gaagatagag gacacgccgc gccgcgccag cgcttccacg ctggcgcggc ccagcccgcg
     2161 gctgccgccg gtgatgatgg cgataggcga ttggttcatg acgaaggctc cttgatgggg
     2221 tgccgctttt tgccaccctt gccccctcgg cgataagaca gggcttgccg gccagattgt
     2281 tctgccgcac aaaacaatca ccatggatcg cctggcaacg ctcgaattgt tcacccgcat
     2341 tgtcgagcgc ggcagctttc gcgccgcggc cgccagcctg ggcgtgtcgc gcccggtggc
     2401 cacggcggcg atcaaggcgc tggaacagcg gctgggcgcg cggctgctgc agcgcaccac
     2461 ccgccatgtg cgccccaccg ccgagggcga ggcctattac cggcgctgcc tggccatcct
     2521 cgccgatctg gaagcggccg agcgcgatgt gaacggggcg gtggccggcg tggtgcgggt
     2581 ggatgtggcc gggcgcctgg cgcgcgggct gctgctgccg gcgctgccgg cctttctggc
     2641 gcgccacccg gcgctcaccg tctatctggg cgagggggag cgttttgtcg atctggtgcg
     2701 cgaaggggtg gattgcgtgg tgcgcgcagg gccgctggcc gacagcgaca tgatcgcccg
     2761 ccagctgggc gtgatggagg agatcacctg cgccagcccg gcctatctgg ccgcccatgg
     2821 cgtgccccgc agcccggatg atctggccgg ccacagcatg atcggcttcg tctcctcgcg
     2881 cacggggcat cctctgccgc tggaatttgc cgtggccggg caaatggtgg aagtggcgct
     2941 gcccgcccgc ctgctggtga acggggcgga aagcagcgcg gccgctgccc ggctggggct
     3001 ggggctgatg caggcgccgc gccacgggct ggccggcgat ctggccgccg gcgcgctggt
     3061 ggaagtgctg gctgaatatc gcccggcgcc aacgccggta gcgctgctct atcccagcaa
     3121 ccgccagctg tcggcccggg tgcgggtgtt tgccgattgg ctggtggcga tactggcgcc
     3181 cgctctggcg gcgggctgaa cggccgggtc agcccgccgg cgccacatcg atgcaagccc
     3241 gcgccagccg gcccatcgcc gggcggtggc tgatgaggac aaggccagtg ccagtcctgt
     3301 ccagccagcc ttccagccgg gccgccagca gcgcctcggt ggccgcatcc agcccctcgc
     3361 tgggctcgtc gagcaccagc cagggccggc cggccagcag cgcccgggcc agcgcgatgc
     3421 gccgccgctg cccgcccgac aggcgcgcgc catcgccgcc cagccactgg tgcaggccat
     3481 cgggcagggc gcgcaccacc tcgtctgcca cagcgatgtg gagcgcttcc cacatctgcg
     3541 cctcgctcag ccccggccgg gcgagggcga gattgtcggc cacgctgccg gccatcagcg
     3601 gggcttcctg cgctgccagc gcgaaacagg ggcgcagcgc cgccagcccc agcgcgcgtg
     3661 gatcgctgcc ggccacggcg atgtcctgcg gggcatcccg cctcaggccc accagcgttt
     3721 cggccagccg cgtcttgccg gcgcccgatg ggccgcacag ctgcacccgc gcatcgggcg
     3781 ccacctgctg gccgcacagg cgcagcgccg gggcgctggc ggcagtggcc atgggcgccg
     3841 gatccggggc ctggctgatc gctgccagat gcgcctcggc cagcgccagc tggtgcccgc
     3901 gcatgtctgt ctgcgcgagg gctgcccagc cttccagcgc ggccattgcc cccaacaggc
     3961 ccagcgcgca cagcggcgcg ggcgctgtgg ccacggcggc gagcgcagcg gtggccagcg
     4021 ccgccatcgc cgtctgcccg gccaggatca gcccttcggc gcgggccagc gctcgccggg
     4081 cggcatcctg cgcgcgggcg gtttcggcca gggcggcggc catcgccggc gccagcccat
     4141 agacggcgat atcggcgcag ggcccggcga gatcggcata atcggcctgc agcgcggcat
     4201 gggcggcggc ggcctgtgcc agcggggcag gcagcaggcg cgcggccatc tgccgcccgg
     4261 ccagccgcat ggcggccatc gccagcccca gcaccagcag cgccggccag cccagtatgg
     4321 ctgccgccgc cagcccggcg cctgctgcca cccaggcgcc ggggcgcgat atctggcgga
     4381 tcaccgcatc ctccagcgcg tccacctcct tgcccagacg gttggccatg gcgccgctgc
     4441 gcccgatatt ggcatcggcc agcgcccgcg ccgccacgcg cccgaacagc gccatgcgca
     4501 gctcagccag cgcgaaaagc gccgcccggt ggcccagcat gcgctcgcca tagcgggtgc
     4561 cggtgcgcaa gatggccgcg gcccggatgg cagcgctggg cagcagatag ttgaaggcct
     4621 gcaccgccag cacgccgccc atccccgcca gcgccgcccc ggccagaaac cagcccgaca
     4681 gcgccagcag ccccaccgcc gccagcgccg cgattcccgc acacaggatg cccagccgca
     4741 cgaagcgccg ctggccgcca atggcctgtt gcagcagcac gttcatggcg ccacctcgca
     4801 gacagaggcg gccatgccga tcagcgctgc atcatggctg gcggcgatca ccagccggct
     4861 gcgggccagc tctgccagga gggcgcgcac ggcccgggcg gtggcggcat caaggtcggc
     4921 ggtcggctcg tcgagcagca gcagcggccg gcccgagagc accgcgcggg caaggccaat
     4981 ccgccgccgc tcgccgcccg aaaggctgga gccgcgatgg tctatcgcca tctccagccc
     5041 gccgcgggcg cgcagcatcg cccccagccc cagcgcttca aggcatggcc gcatggcacc
     5101 atcggacggc ggatgggccg cgcccggcgc cagattggcc ctgagggtgc ccggcagcag
     5161 cgccacctgc tggcccgccc agccgatggc atggttcagc gcgcccggcg caaaggcgtg
     5221 gccatcggcg cggattgcgc cggcgcccag cggcacctgc ccgatcagcg ccagcagcaa
     5281 cgaggatttg cccgcccccg tcggccctgt cagcaggtgc aggccagggc cctgccagtc
     5341 ccagtccagc gggccgatcg tggtgccatc gccatggcgc agcaccaccg cgcggccctg
     5401 ccagcgcagc ggcgcggcga tgggcggcgg tggcggggcg ctggcggcca gctgcgcggc
     5461 catggcggcg atggcggcct cgccctgctg cttgtcgtga taggccgctg ccagccggcg
     5521 catcgccaga tagaattccg gcgccagcgc cagcgcatag aaggcgcgcc acaggctcag
     5581 atgttctggc gcggggaagg gcagcaggcc cagcagcgaa aagccgcaat agaccgccac
     5641 cagcgccacc gccagcgccg cgaagaattc gagaatggcg ctggaggcaa aggcaatcgc
     5701 cagcaccgcc atcgtgcgcc gggccacttc ctgcgcggcg gcgcccagat ggcgcgtcac
     5761 ccgcggctcg gcagcgaagg agagcaccat cggcagcgcc cgcagccggt cgacgaacag
     5821 gcccgacagg cgcgcaaggg cctggtgctg ggcctgcgcg cgggtggccg ccgcgccgcc
     5881 cgccagcgcc atcgccagga taaacggcac cagcgttgcc agcaggatca ggctggccac
     5941 ccagctggcc ggcgccaccg ccagcgcaat gagcagcggc gtcgccccgc tggccagccg
     6001 cagcggcagg aagcgcgccg ccagcccttc tgtcgcggcg atggcatcga tggcgatatg
     6061 catgtcctcg ccgatcagcc ggccgcgcat cagccggctg ggcagcaggg cagggtgcag
     6121 cacgcggcgc agcgctgctt gcagccgtgc cgccgcgccc gcgcctgcca cctgcgcgcg
     6181 ccacagcgcc gccgcccgca gcagcccgcc gcccgccagc agcgccaggg cgggccccgg
     6241 cgcgctgccg gcgcctggca gccgcgccgc caccaccagc gccacgccag cggccaccgg
     6301 caccgcgccc gcactgtcca ccagccacca gaaagcggcg cgcctatcga ctttggtcgg
     6361 ggggagggag ggggtgtctg cgctggacat attaaggaag gcgcatatta gggccctgcg
     6421 taattggcaa gcgagtcctg tcgcatcaac cctttgccga ttgcctctcc cagcgcccct
     6481 ggggcgcgct tccaagggaa catgtccgat ggatatggcg gtcatcgaat tatcccggct
     6541 gcagttcgcg ctgacagcga tgtatcactt tctcttcgtg cccctcacgc tgggcctctc
     6601 cttcatgctg gtgatcatgg agagcgtcta tgtcatcacc aagcggccga tctggcgcgt
     6661 gatcacgcgg ttctggggca ggctgttcgg catcaacttc gtgctgggcg tggcaacggg
     6721 ccttaccatg gagttcgaat tcggcaccaa ctgggcctat tacgcgcatt atgtgggcga
     6781 catcttcggc gccccgctgg cgatcgaggg gctgatggcc ttcttcctcg aggcaacgat
     6841 ggtcggcgtc atgttcttcg gctgggatcg cctcagccgg cgccagcacc tgctcgtcac
     6901 cttcctcacg gcgcttggca ctaacctgtc ggcgctgtgg atcctggtgg ccaatggctg
     6961 gatgcagaac cccgtgggcg ccagcttcaa cccggccacc atgcgcatgg aagtgacaga
     7021 cttcaaggaa gtgctgttca accccgtggc ccaggccaaa ttcgtgcaca ccgtctcggc
     7081 cggctatgtc tgcgccgcca ctttcgtgat gggcatctcg gcgctttacc tgctgcgcgg
     7141 caaatacacc aatgtggcgc gccgctcgat gatggtggcc tcggccttcg ggctggcctc
     7201 ggcgctgtcg gtggtggtgc tgggcgatga gagcggctat gcgctgaccg acaaccagaa
     7261 gatgaagctg gccgccatcg aggcgatgtg ggaaacagag ccggcgccgg ccgccatcac
     7321 cctggtgggc ctgcccgaca ttgcgcagcg caccacccat catgcggtga agctgcccta
     7381 tgtgatgggg ctgatcggca cgcgcagcct gtccggcaag atcgatggca tagaggaact
     7441 ggtgctgcgc gccgatgggc gcatccacag cggcctcatc gcctatgacg cggtggaaaa
     7501 gctgaagcag aaccgcgccg acaccgccgc catggccgcc tttgaaggcc acaagcgcga
     7561 tatcggcttt gccctgctgc tcaagcgcta tgttgctgat ccgcgccagg cggatgaccg
     7621 gctgatcctg aaggcggcat gggacacggt gcccaacgtg ccggtgatgt tcttcctgtt
     7681 ccgcttcatg gcggggctgg gctttgcctt catcggcttt ttcgcgctgg ctttctattg
     7741 cgcctcggcc tgccgctttc gcaagcgctg gttcctcaag ctggcggtgt gcatgctgcc
     7801 gctgccgtgg gtggctatcg aattcggctg ggtgctggcc gagatcggcc gccagccctg
     7861 ggcggtggat ggcgtgctgc ccaccttcct gggcgcctct tcgctgagcg tgccgcaagt
     7921 gtgggccacc attttcggct tcaccgcgct gtatggcacg ctggcggtga tcgaggtgcg
     7981 gctgatgatc gcggccatcc gcaaggggcc gatcgaacac cacgaggacg atgggcaggc
     8041 agaggcgggc tttgccccgc tgcccgccga ataagggagc cagacagatg catatccctc
     8101 tcgactatga aaccctgcgg ctgatctggt gggtgctgct gggcgtgctg ctgatcggct
     8161 ttgcgctgac agatggctat gatctgggcg tggccagcct gctgcccttc gtggcccgca
     8221 ccgacgagga acgccgccag gcaatcaacg ccatcgcccc ccattgggaa ggccatcagg
     8281 tgtggctgat cctgggcggc ggggccatct ttgctgcctg gccgtttgtc tatgcggtca
     8341 gcttttccgg cttctatctg gccatgttcc tggtgctggc cgcgctgatc ctgcggccgg
     8401 tgtccttcaa atatcgctcc aaacatgccg atgcgggctg gcgcgcgcgc tgggactggg
     8461 cgctgtttac cggcggtttc gtgccggcgc tggtgtttgg cgtggcggtg ggcaatgtgc
     8521 tggcaggcgc ccccttccgc ttcgacagcg acctgcgcat gacctacgag ggcaccctcc
     8581 tcggcctgtt cacgcccttc accgtgctgg ccggcttgct gtcggtggcg atgctggcga
     8641 tgcatggcgc ggcctggctg gcggtgaagg tggcagacgg cccggtggtg gcgcgcgcgc
     8701 gccgctttgg cctgtgggcg gcggcggcca gcgtggcgct gttcgcgctg ggcgggtgga
     8761 tggtggcgca tggcggcatg ggcatgcggc tggtgggcgt ggccgatgcc ggcggccctt
     8821 ccaaccctct gttgacggcc agcacctttg cccccggcgc ctggctcgac aatttcgccg
     8881 cccatccctg gatggtggcc gcccctgtgg ccgggctgct ggcgcccttg ctggcgatgg
     8941 cggggatcgc cgggcggctg ggctggctgc agctggccgg atcggcgctg tcgatcaccg
     9001 gcatcatcgc cacggtgggc tgcgcgatgt tccccttcat cctgcccagc tcgatcgatc
     9061 cgcgctccag cctcacggtg tggaatgctt cctccagcca tctcacgctg ttcatcatgc
     9121 tggtttgcac ggtggttctg ctgccgatgg tgctggccta cacggcctgg gtgatgaagc
     9181 tgctggccgg ccgcatcact cttgccgaag tgcgttccaa ccccgatttc tactgaaagg
     9241 acaaggccat gtggtatttt tcctggatcc tggggctggg cctggcggtg ggctttggca
     9301 tcatcaacgg gctgtggcac gaattcaact cgccgatcga cgatgatccg gccatctgat
     9361 tatcccgctg tgtaagcgcc agccccgggc cagccagacc ttcccggcca gccatggcca
     9421 tggtgctccg cttgcgacag gcccccgcat atccccgccg gatatgcggg ggttttgcca
     9481 tgccggccgc ccatctcgac gctgcccgcc ctttcgctta tactggtccg tcatgcgctc
     9541 ttgcgagatg gcaaggcccc aactgcggat tgacgatgaa gcctgccgac ctgaccgaac
     9601 tgaaagccct tgccgcgcac atggccgggc gcctgaaggt gatgagccac cccgagcgct
     9661 tgctgatgct gtgccggatg gacgagggcg aggtcagcgt gaacgagctg gtggcgttga
     9721 ccggcctgtc gcaatcggcg gtgtcgcaac atctggcgat gctgcgcgcg gaagaggtgg
     9781 tgggagtgcg gatcgatgcc aacatccgct ggtatcggct gacggacccg atcgtctcgg
     9841 gcatcatcca tgccctttgc gccttgtgcg agccccaagc ggcgcagcca taggcgcaaa
     9901 aggcggcgca ggtggggaaa atggtgggcc cggcaggatt cgaacccgcg acctagccgt
     9961 tatgagcggc cagctctaac cgctgagcta caggcccacc tggccggccg gcccgtcggc
    10021 cggcggcgtg gccctgcgtt agcgggcaca ggcgcgctag gcaagctcca cactgcccat
    10081 gatatcggca accgagctgg ggcaggcccc cagcgcgcgc aattcggcaa aaaccgcccg
    10141 ccaccagtcg tacagcttgt cgagatcgtc ttccgagcgg acatagggcg tgtgccccgg
    10201 gctgagcgag gggctgtgaa acgacagcac gatcaccggc agctgctctg ccacggcctg
    10261 gcgcacgccg cgcagcgctt cggcaatggt caccccctcg ggcgtcagcg gaatgcggtc
    10321 catcatcccc agccttgcca gcacgctgcg catctgcggc aggcgccaca gccgcggata
    10381 gagccactgc gcccggcggc gcagatggcc ggtgaacagc gttgtcagcg gcagttccat
    10441 cagcgtgcgc ccttcatcca cccaccacgg cgtcagcggg cgggtggcat aattgggccc
    10501 gccatgcgag ctgtagtcga agcgcgaacg caccgatgtg tcgatcgcga tgcccgcctc
    10561 gcgcaggatc tgcgccgtgt tcggccccag cccatagcgg ccggcgcgat agatgcgcgg
    10621 ggtctggccg aaattgcgct cgatcgtgtc gcgcaggctc atcagcttgg cgcgttccag
    10681 ctcgtagggc agattgccgg caaagctgtt gaactgcgtc acctgctcct cgaagggcgg
    10741 gttcacccag gggtgcagct gcacgccgat ctcggcccgg ccggcggcca gcggcgcgcg
    10801 cagcaggtct gcggccagtt cggatatggc gatgggatag tcgatcagat agatcggcac
    10861 cacgccctgg ccctcgcaga attcctggaa cttgcgcagg cgcgccaccg tatcggtgct
    10921 gtggccgccg cgttccagcg ggcggctcca gtcgaattcc tcctcggtgt ccaccgtcag
    10981 caggaagcgg gggccaggcc ccccggaaaa tcgtacatcc gagcccggag cgggcgcaga
    11041 catcagggac gagttaggca agtcgcacct ctgccagacc acgcaggaca catcgaacgg
    11101 ttcatgccgc aggggcggcc cccaccgcct gcccgcgcgc aggatggcta ggcggcgcag
    11161 cggcagcggt caagccgggc aggcgcaatt gcagcgccgc gccctggcgc agcagatcgc
    11221 cgcctgccgc ccgtgcctcg gcgctggcca gccgcagggc aaagccggtg ccaaacatgc
    11281 cggcaaaggg cgcctcgggc ccggtgctgc cctgtgcgtg atagaaggca tgatcatcca
    11341 gccctgccag cgtgcgcggc aaggccatgg ccagcacctt gtagcctccg gtcgccacca
    11401 gctccaccgg cagcacttcg cccgggcccg cattggccgc cagcgttgcc agcaggcgcc
    11461 acaccagccg ctccagctct gccggggcca gcgccacggc cagcggcggc gtgccatcgg
    11521 cctctggggc aggcggcgcg cccagctgga agccgctggc gcgcggcgtg ctgtgcggct
    11581 ccagccgggc gatggtggcg gccacgcagg caaacagatc gcccgatccg gccggcagcg
    11641 ccatcgcccc gctgtccagc ttggcaaagc gctccagctc ttcaaagccg gccagcatgc
    11701 gcgcgctgtc ggccgcgata tgggcggcca gtgcgcgata ttcatgcggc gcggggccaa
    11761 acaactgctg ctggatgatc tcggcaaagc cctgcacggc gttcaccggc gtgcgcaatt
    11821 cgtgcagcaa ctggcgcagg cggtcgctcc ccgtctcgcg cgggggcggg ggggcggtgc
    11881 cggctgccgg cc
//
LOCUS       DASRYE010000040        19298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_89645,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000040 DASRYE010000000
VERSION     DASRYE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19298
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89645"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1371)
                     /locus_tag="VFF98_02160"
     CDS             complement(58..1371)
                     /locus_tag="VFF98_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU62460.1"
                     /translation="MPAVALALRAPLFHTPARRVLEHIPDALVEVDGAGTIIAVGPAL
                     PARVAELAAAGVLTTLPEGHYLLPGMVDLHAHAPQWPQAGRALDVPLEEWLMRHTFPL
                     EARYADLGFAGPVYADLVGSLLAHGTTTAVYFGTVDVAPTVLLAQTCLARGQRALVGK
                     VAMDVPGECPDFYRDADADAALAGTAETIAAIRALPGNAGALVRPVITPRFLPTCSEA
                     LLRGLGDLAEATGAPVQTHCSESDWEVAHGAARFGCSDAAALERFGLLRAHTVLAHCT
                     HLAAGDPALIRAAGAAIAHCPLSNVYFAGEVLPAAPLLAEGLRIGLGSDIAGGHAPSM
                     LEAARFALTAGRVRTRGAMAGAPGVAEALWMATAGGAEALGLATGHFAPGLAFDAIAL
                     DALSPECLAWPGIDTPADVLEKLLRRAGLAQIRHVWVAGRRVAGG"
     gene            complement(1374..2147)
                     /locus_tag="VFF98_02165"
     CDS             complement(1374..2147)
                     /locus_tag="VFF98_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU62461.1"
                     /translation="MNRPLAQRLAPILFIAALLALWEGVVRLNHVPSFILPTPSQIAI
                     RLVEDWPILWPALVYTCGVTAAALIASGVLGLMLAMLMSASAWVERMIYPLAVVLQVT
                     PIVAIAPLIIIWVHDTRLSLLICAFLVAFFPVVANATTGLNSVDGNLRALFTLYGARP
                     WQRLFWLSLPSALPYYLAGLRISGGLALIGAVVAEFVAGTGGTRSGLAFVIVESGYRL
                     SMDRMFAALFLISASGILLYALISRITRALLRHWHESAL"
     gene            complement(2144..2959)
                     /locus_tag="VFF98_02170"
     CDS             complement(2144..2959)
                     /locus_tag="VFF98_02170"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62462.1"
                     /translation="MTRPLLTIEGLAKSWRRPGGGLHEVLAGVSLSVPAEPQFISLLG
                     PSGCGKSTLLRLVAGLDAPSAGRIDWSQAAFEAFGAARPSVGFVFQEPTLMPWASLFE
                     NVRLPLRVAGRSRREAREEVMAALDMVGLADRAGAYPRQLSGGQKMRVSIARALVMRP
                     RVLLMDEPFAALDEITRHRLNDDLLSVWQASGATVLFVTHSVYESVYLSSRIITIGTG
                     GAGPGRIIADRAVDAPYPRDEAFRTSDRYNALCRQVSADLRAASGGALEEVHA"
     gene            complement(2959..4065)
                     /locus_tag="VFF98_02175"
     CDS             complement(2959..4065)
                     /locus_tag="VFF98_02175"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62463.1"
                     /translation="MSWPARAARALDLAFHGGIALFFLGCAAALFSDGGAAGGDHITV
                     GLNWVPETEHCGFFEAEKAGLYKAAGLNVEIVNGGPDVNLPLLVGSGRMNLGIGTSFT
                     TLNMQARGIDAITVASFFQKDPQTLVAHGGQGITTLQDMRGRPIMVGNFARSEFWQFL
                     KATQGFTDDQLRPYDSNPSAFLADKTAIAQGYASQDGVMLGTHMPEGMVSLLLADYGF
                     DNYSQTVFGMRGWIEDHHEEVRRFVKATAEGYRRCTYEPLRPGDETTAMPEILRRNPE
                     HGVPLYVFKHGKLRSMGFIGGGDAARLGIGAMTEARWHHFYQTMVKAGIYSPTLNWRR
                     AYSLDYVTPDNRVMPPAATPPAAQPPAQTPEGGR"
     gene            complement(4062..4730)
                     /locus_tag="VFF98_02180"
     CDS             complement(4062..4730)
                     /locus_tag="VFF98_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU62464.1"
                     /translation="MLILHTSDLLAEAYAARLLASLAGVALEIRHLDIHPGRDHETDA
                     FRVLSPLGRLPVLEGAGAPLADWQAVLCAIATRGDTPARADAPGWWRGADPAFIGWLG
                     MARDLAASAGVARLHEGLGVPADGAALRAAAHALLREVERHLWFAERAGQRWLLAGEG
                     PCAADIACYVHIALCEEGGIARMDYPAIRRWVDRIRHLPGYIVTSGTFAPPAPAPVRQ
                     PVRA"
     gene            complement(4724..5335)
                     /locus_tag="VFF98_02185"
     CDS             complement(4724..5335)
                     /locus_tag="VFF98_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZU62465.1"
                     /translation="MSGRGPWVPVAASADLAPGHVYQTMLAGQGLALWRGDDGAVNVW
                     EDRCPHRGVRFSAGDVVGGELRCVYHAWRFAAGSGACTFIPAQPNAKVPRAIHATPWP
                     VAEVAGLVWTALAAQGAPPADLAGEVLRALPVNRPAAAVAQEAAALMAAHPALRLFVQ
                     PLAADRAVIRGLAAGVDIMAADLLLEAARRRLEDAALMEAAAC"
     gene            complement(5332..6219)
                     /locus_tag="VFF98_02190"
     CDS             complement(5332..6219)
                     /locus_tag="VFF98_02190"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62466.1"
                     /translation="MAEILTGAASGARDPVLWNDWHVIADLAALRAGADFATTLLDVP
                     LLARLGAAGAQARRADGAPLHTEVAYGFLWACLGAPARPLITIAEYAEAGRTIATGGS
                     VGVHVAAPRVVENFLDLGHLGYVHVGYLGEEPETMVHPYRVGPLEGGGVLATGCKVYQ
                     PVASPAAQGGYMVDYQYCVVRPLITCLHKANPVARDAWDVIYLFVQPVSEERSIAHTL
                     MLFAPDGTQPADLRRFQQLIFLQDKPILENQTPRRLPLADGAELPIAADSTSVAYRRY
                     LVAEGLAYGVIPAGARVAA"
     gene            complement(6320..7234)
                     /locus_tag="VFF98_02195"
     CDS             complement(6320..7234)
                     /locus_tag="VFF98_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62467.1"
                     /translation="MFSRFALYFDEVVRAGSIRRASERLRISPSAIDRHMLLMEEELG
                     VRLFERMPQGLRLTAAGEVLITYIRRWRREWGHAVAQIDDLLGLRRGEVTVAMAEGAA
                     DFVARAIAPFRDQYPGIDLNFVTAGASVVGEKVLEGQAEIGLTFNAPEGQAFRVERTM
                     VYQLGAVVAAGHPLAGREEVSFADCADHGLVLPDETLSYRQVLDTIWARTIGGRSRGA
                     VNAGSISLLKALVKGGRGVGMLTRLDVSAEEEAGTLVFVPLAEPNIPLSVLSLISASG
                     RTLSVPASLLLQHMAQTMRDSGVGGIGG"
     gene            7470..9914
                     /locus_tag="VFF98_02200"
     CDS             7470..9914
                     /locus_tag="VFF98_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62468.1"
                     /translation="MREFQNPARRQLWRVAGGSGVARGALVAALGSALALAGTAHAAG
                     ANAADAAAADAAPVQGLGDIVVSASKRETDVQKTPLAVTALTADTLTANHVTDAVDLN
                     GLVPSLVITTSEGFERNIAIRGVGFNVPQNDAAQPSVSYHVDGVYIANPVAMNTNFLD
                     VNHLEVVRGPQGTVYGQNSTGGTVNVVTNQPQLGKFGGFVKFDTGTYALAQFEAAANV
                     PLGDTVAIRAAIQAVHHDGYAVATKVPGTNGHYGLSDENSMHGRLSLLWAPTDRLSLM
                     LRAEYAHANNHETEGKSIYDTDPNPWHQSSDWPGKIAYDNSMFTGTLNYDMGWAKLKA
                     LGSWQMVRQNGSVNEDGLDLAGAIAAYGVTGFDSVHDIEYLYHRSTAKTGEIDLASPA
                     NQMLEWIVGGFYLSSNYRVSYDQYAVDSYPVSGQPTSLQNQIFPAVTNGDNLGFEGAD
                     GNFYYPYFYSISYLTRESYSLYGQATLHLSSRLRLSGGVRYTHDYNSTSICDYACDTP
                     TFVQQSSNAWTGKAGVEYDLARKTMAYFTWSTGFKPGGGNISPTPIVLGYTFKPETVT
                     AYELGAKSTMLGGRLRLNVAGFYYDYSNTQFEAEDAVAYQGGVANIPKLHIYGVEAEA
                     SALLGGGWRFDGNVTIEDSKITSHYMALDNVTGYNIDKQIFASGNSVFYDPGRSADIA
                     ARTAAYFDVYGNQAPNLPHVSATGTLSQKSDLPWGGALTSSLQGEYRSSYVNAVYGDM
                     VINGVHVYRSPAYNLWNVNFTWQPVNPHFKFSASLKNIFNSGAVLGQFTNQFGGEITR
                     QYAPPRRLIVSAAYNF"
     gene            10072..11379
                     /locus_tag="VFF98_02205"
     CDS             10072..11379
                     /locus_tag="VFF98_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZU62469.1"
                     /translation="MAARGTRLPTEILAGCTTFFTMAYIIVVNPSILGLAGLPVPSVA
                     MATCLSAGLACILMGLIADVPLALAPGMGLNAYFSFTVVQQMHVPWQVGLGCVFLSGL
                     AFLVLTAAGIRQMIVAAIPHYLLAAVAAGIGLFIAFVGMKNAGLIVASPATIVALGNL
                     REGGALLAVIGLVLITVLSLLRVRAAILIGIIATTLIGWLTGLATAPATAPSPAGLGA
                     TFLALDIPGALHIGSKGAGIVGLGLVEIVFVFLFVDLFDNIGTLVAVTKRAGLMEADG
                     TIPALNRMLYADALATLAGALLGTSTVTSYVESTAGVESGGRTGIAAIVTGLLFLLAL
                     PLARYAQLVPLSATAPALILVGALMMAPLTEIAWEKAEEAVPAFLTLAMIPLTFSIAN
                     GLAFGITAHALVKIIKREVAARDWLLFVLALLFVIRFAYISAA"
     gene            11389..12972
                     /locus_tag="VFF98_02210"
     CDS             11389..12972
                     /locus_tag="VFF98_02210"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZU62470.1"
                     /translation="MPTLSASSAKGLVVAPHFAAAEAGRAVLAAGGNAVEAAVAVAAT
                     IAVVYPHMNSIGGDGFWLIHRPGQPMTGVDACGRAAAAADLALYAGHATVPWRGPLAA
                     NTVAGALSGWARALEIAGGSLPLESLLATAIAHAEEGIAVTAGGAANAAAKSAELTAC
                     PGAYAAIFEPDGRPLREGEILRQPALAQTLRRLAAEGLDSFYRGALAADIAADLAALG
                     SPVSAADLAAHVAEESLPLASRIAGATLYNHRPPTQGIASLLILTLADRLAKGSEGSF
                     THVHALVEATKQAFLWRDRHCGDPAYMDEAAQDLLDDEPRIAAMAAAIDPARALPWPQ
                     PSQQGDTVWFGAADKDGMVVSLIQSTYFEFGSGLVLPQTGITWQNRGASFRLAESGWN
                     ALKPGRKPFHTLNPALARFDDGRVMAYGTMGGEGQPQTQAAVFSRHVWHGLPLADAIA
                     APRWLLGRTWGEDSTSLKLEEGFAPELYAQLAAAGHQVEILPACNSTMGHAGAVLRHP
                     DGLIEGASDPRCDGMAAAC"
     gene            12966..14216
                     /locus_tag="VFF98_02215"
     CDS             12966..14216
                     /locus_tag="VFF98_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HZU62471.1"
                     /translation="MLSAAHHAAGARATQRCRALGIAPYSDLADALYRAWLTPAHKAA
                     LGALEGWMTEAGMAVRIDAMGNCIGRYEGTAPDAPTLLIASHIDSVKNAGCFDGPLGV
                     MLGVECVSALHAARRRLPFAIEVAAFGDEEGSRFPAAMLTSRAMAGTLDTADLAIADA
                     QGTSVAEALATFGLDIARAAEARLPYTPIAYLEAHIEQGPVLEAEGLALGTVTGIAAQ
                     LRYEVQVEGQAGHAGTTAMPLRRDALAGAAEMIAAIEEIARAAASDVVATVGRIEARP
                     GAANVIPGAVRFTIDVRSGAEGRRDAAAAGILAAIGAIAGRRGLAARWEEINNLPASP
                     CAEPLMALMDSAIASTGQRPFRLVSGAGHDAMVMAALCPVAMLFIRCEKGISHNPAEN
                     VEVADVAAGFAAMMAFIDSLGDTL"
     gene            14273..15460
                     /locus_tag="VFF98_02220"
     CDS             14273..15460
                     /locus_tag="VFF98_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZU62472.1"
                     /translation="MGPGPVNAHPRVLRAMSADLLGQFDPEMTGYMNEVMALYRPIFG
                     TANRWTMLVDGTARAGIEASLVSLVQPGDTVLVVNFGRFGLLLQEILTRIGARIATVD
                     APWGEVVPLEAIRAAAQKVGPKVIATVHGDTSTTMAQPLDGIGAIAREVGALVYVDAT
                     ATIGGMELAADRWGADILTGGLQKCLGGPSGSSPITVSDAAAAHIFTRRHIEKGIRAA
                     DAEDGEGAMIGSNYFDLAMIMDYWSEKRLNHHTEATTMLYGARECARVALAEGLAARY
                     ARHRAAGAAMSAGLQAMGLTLFGDDAHRMTNVTGVYIPAGVDGEAIRTAMRRHFEIEV
                     GTAFGPLVGKIWRLGAMGYNAMKHKVLITLAALEACLAAEGFSLPRGAAVPAALAAWE
                     ASR"
     gene            15457..16368
                     /gene="puuE"
                     /locus_tag="VFF98_02225"
     CDS             15457..16368
                     /gene="puuE"
                     /locus_tag="VFF98_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HZU62473.1"
                     /translation="MSTPNTTPRDLVGYGATPPAAQWPDGARIAVNFVINYEEGAENC
                     VLNGDELSEGFLSDMVGAARHPDRAMAMESLYEYGSRAGFWRLHRLFTGRGLPVTVFG
                     VARALAMNPAAVAAMQAADWEIASHGLRWIDYQYTPEEVERAHIAEAIALHTQVTGSR
                     PQGWYQGRTSPNTARLVAEEGGFLYDADSYADDLPYWDASHGRAQLIVPYSLDANDMK
                     MVALNGFTEGEQFFHYLRDTFDYLHAEGGRMMSVGLHGRIVGRPARAAALARFLDHIA
                     TKQGVWVARRCDIARHWAATHPAPELA"
     gene            16368..16760
                     /gene="hpxZ"
                     /locus_tag="VFF98_02230"
     CDS             16368..16760
                     /gene="hpxZ"
                     /locus_tag="VFF98_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HZU62474.1"
                     /translation="MILNDPTLLAEMQAAFAEYERALMADDVPAMDALFHDAPTTNRF
                     GVGEVLWGAEAIRAFRKGRGGSPQRRLGHLAITTYSTPDGAAFATADVEFFRENSERR
                     GRQSQAWVKFADGWKVVSAHVSLEGNSH"
     gene            16760..17248
                     /gene="uraD"
                     /locus_tag="VFF98_02235"
     CDS             16760..17248
                     /gene="uraD"
                     /locus_tag="VFF98_02235"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZU62475.1"
                     /translation="MPVDFNALSDADFIARYGALFEHSPWVVERAARLRPFADLHEGL
                     MRAVHEASRDEQLALIRAHPELAGKAAIAKELTDASTAEQASAGLDRLTAEEYARFHA
                     LNAAYRAAHAMPFIICVRLTDKAGILAAMAARTALATDDEIATAIAEIGKIVALRLED
                     LA"
     gene            17245..18447
                     /locus_tag="VFF98_02240"
     CDS             17245..18447
                     /locus_tag="VFF98_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZU62476.1"
                     /translation="MTLLSHIDLLEWLNLALRWLHVITGIAWIGASFYFVWLDNHLKP
                     PAGGAVPGGPSGELWSVHGGGFYHNQKYPVAPPRMPDELHWFKWEAYFTWMSGFSLLV
                     LVYYVGAKSFLIDPAKVALSQPQAIGIGLAFLAGGWLVYDGLCRTLARSPRLLGAIWF
                     AVLVGAAYALNLLFNARGAYLHVGAMIGTVMAANVFLIIIPNQRKVVAALVAGETPDP
                     ALGAAGKLRSVHNNYMTLPVLFIMISHHYPMTYGADRPWLVLALLGATGVAIRQVFTL
                     RHKHAPTGRWIALAVVLALASVTYVAAEKASHEEGTAGNVTYADIQPILARHCYACHG
                     AHPTNPAFPSPPLGVKLDTAEHVAAVAPRIKAVAVEAVVMPLGNSTGMTKAERAQIGA
                     WIAAGARP"
     gene            18444..18770
                     /gene="uraH"
                     /locus_tag="VFF98_02245"
     CDS             18444..18770
                     /gene="uraH"
                     /locus_tag="VFF98_02245"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HZU62477.1"
                     /translation="MSSLSTHVLDTASGQPAAGLRVVLSAGGTVLAEGLTDADGRFRG
                     FPPLAAGAYVLTFHAGDYFRAAGVALADPPFLDIVPIHFGAGAEGHYHVPLLIAPYAF
                     STYRGS"
     gene            18772..>19298
                     /locus_tag="VFF98_02250"
     CDS             18772..>19298
                     /locus_tag="VFF98_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU62478.1"
                     /translation="MGEIRFLLNGTQRAIADANPTATLLDWLRYDARLTGSKEGCAEG
                     DCGACTVLVGERTQGDAVQWRAVNACIQFLPMLDGKAVLTVEGLAEGGALAPVQAALV
                     AHHGSQCGFCTPGIVMSLAAKAFGALGTEQASTGDVLQGNLCRCTGYGPILAAGDGLA
                     AAAAPDVAAQLAALAR"
BASE COUNT         3127 a         6768 c         6432 g         2971 t
ORIGIN      
        1 tggcggccgc gctgggccgg ttgcgcgcgc aggccgcagc gcccaagccc tgaagcgtca
       61 gccgcccgcc acccggcgcc cggccaccca cacatggcgg atctgggcaa ggccggcccg
      121 gcgcagcagc ttttccagca catcggccgg cgtgtcgatc cccggccagg ccaggcattc
      181 gggcgacagg gcatcgagcg cgatggcatc gaaggccagg cccggggcga aatggccggt
      241 agccaggccc agcgcctcgg cgccgccggc agtggccatc cacagcgcct ctgccacccc
      301 tggcgcgccg gccatcgcgc cgcgcgtgcg cacccgcccg gcggtgaggg caaagcgcgc
      361 cgcctccagc atcgatggcg catggccgcc ggcaatatcg ctgccaagcc cgatacgcag
      421 cccttcagcc agcaggggcg cggccggcag cacttcgccg gcgaaataga cgttggagag
      481 cgggcaatgg gcaatcgcgg ccccggccgc gcggatcagc gccgggtcgc ctgcggccag
      541 atgggtgcag tgggccagca ccgtgtgggc gcgcagcagg ccaaagcgct ccagcgccgc
      601 cgcatccgag cagccaaacc gcgccgcgcc atgggccact tcccagtcgc tctcactgca
      661 atgggtctgc accggcgcgc cggttgcctc ggccagatcg ccaaggccgc gcagcagcgc
      721 ttcgctgcag gtgggcagga agcgcggggt gatcaccggg cgcaccagcg cgcccgcatt
      781 gcccggcagg gcgcggatcg cagcgatggt ttcggcagtg ccggcaaggg cggcatcggc
      841 atcggcgtcg cggtagaaat cggggcattc gcccggcaca tccatcgcca ccttgcccac
      901 cagcgcgcgc tgcccccggg caaggcaggt ttgcgccagc agcaccgtgg gcgccacatc
      961 caccgtgccg aaatagaccg ccgtggtggt gccatgggcc agcaggctgc ccaccagatc
     1021 ggcatagaca gggccggcaa agccgagatc ggcatagcgc gcctccagcg ggaaggtgtg
     1081 gcgcatcagc cattcctcca gcggcacatc cagcgcgcgg ccggcctgcg gccattgcgg
     1141 cgcatgggcg tgcagatcaa ccatgccggg cagcagataa tgcccctcgg gcagggtggt
     1201 cagcacgccg gccgccgcca gctcggccac cctcgccggc agcgcagggc ccacggcgat
     1261 gatcgtgccg gcgccatcca cttccaccag cgcatcgggg atgtgttcca gaaccctgcg
     1321 cgccggcgtg tggaacagcg gcgcgcgcag cgccagggca acagcaggca tggttacagg
     1381 gcgctttcgt gccagtggcg cagcagggcg cgggtgatcc ggctgatcag cgcatacagc
     1441 aggatgcccg aggccgagat caggaaaagg gcggcaaaca tgcggtccat cgacaggcga
     1501 tagccgcttt cgacgataac gaaggccagg cccgaacgcg tgccccccgt gccggcgaca
     1561 aattccgcta ccaccgcgcc gatcagcgcc agcccgcccg aaatgcgcag gcccgccaga
     1621 taatagggca gcgccgaggg caggctcagc cagaacaaac gctgccaggg ccgcgcgcca
     1681 taaagggtga acagcgcgcg cagattgcca tccacgctgt tcagccccgt ggtggcattg
     1741 gcgacaaccg ggaagaaggc gacgagaaag gcgcagatca gcagcgacag gcgcgtatca
     1801 tgcacccaga tgatgatcag cggggcaatg gcaacgatgg gcgtgacctg cagcaccacc
     1861 gccagcggat agatcatccg ctccacccag gcagaggcgc tcatcagcat cgccagcatc
     1921 agccccagca cgcccgaggc gatcagcgcg gccgccgtta ccccgcaggt gtaaaccagc
     1981 gccggccaca gtatcggcca gtcttccacc aggcggatgg cgatctggct gggcgtgggc
     2041 aggatgaagg agggcacatg gttcagccgc accacccctt cccacagcgc cagcagggcg
     2101 gcaatgaaaa ggatgggggc caggcgctgg gccagcgggc ggttcatgcg tgcacctctt
     2161 cgagggcgcc gccactggcc gcgcgcagat cggcggacac ctggcggcac agggcattgt
     2221 agcggtcgga cgtgcggaag gcctcgtcgc gcgggtaggg cgcgtccacc gcccggtcgg
     2281 cgatgatgcg gccgggcccc gcgccgccgg tgccaatggt gatgatgcgg ctggacagat
     2341 agacgctttc ataaacgctg tgcgtaacga acagcaccgt ggcgccgctg gcctgccaca
     2401 ccgagagcag gtcatcgttc agcctgtggc gggtgatctc gtcgagcgcg gcaaagggct
     2461 cgtccatcag cagcacgcgc gggcgcatca ccagcgcgcg ggcgatggaa acgcgcatct
     2521 tctgcccgcc cgacagctgg cgcggatagg ccccggcgcg atcggccagg cccaccatgt
     2581 ccagcgccgc catcacctcc tcgcgtgcct cgcggcgcga gcggccggcc acccgcagcg
     2641 gcaggcgaac attctcgaac aggctggccc agggcatcag cgtgggttcc tgaaacacga
     2701 agccaacgct ggggcgggcc gcgccaaacg cctcgaaagc ggcctgcgac cagtctatcc
     2761 gcccggcact gggcgcatcc agcccggcaa ccagccgcag cagcgtgctc ttgccgcagc
     2821 ccgaggggcc cagcaggctg atgaactgcg gctctgccgg cacgctcagc gacacgccgg
     2881 ccagcacttc atgcaggccg ccgccggggc gccgccagct cttggccagc ccttcgatgg
     2941 tgagcagggg gcgggtcatc agcggcctcc ttcaggggtt tgggcaggcg gttgggcggc
     3001 aggcggtgtt gccgcaggcg gcatcacgcg attgtccggc gtcacgtaat ccaggctgta
     3061 ggcgcggcgc cagttcagcg tgggcgaata gatgccggcc ttcaccatcg tctggtagaa
     3121 atggtgccag cgcgcctcgg tcatcgcgcc gatgcccagg cgcgcggcat cgccgccgcc
     3181 gataaagccc atgctgcgca gcttgccgtg cttgaagaca tagagcggca cgccatgttc
     3241 ggggttgcgg cgcaggatct cgggcatggc ggtggtctcg tcgccgggcc gcaggggctc
     3301 ataggtgcag cggcgatagc cctcggcggt ggccttcacg aagcggcgca cttcctcgtg
     3361 atggtcctcg atccagccgc gcatgccgaa cacggtctgg ctgtaattgt cgaagccata
     3421 atcggccagc agcaggctca ccatgccctc gggcatgtgg gtgcccagca tcaccccatc
     3481 ctgcgaggca tagccctggg cgatggcggt cttgtcggcc aggaaggcgg aggggttgga
     3541 atcatagggg cgcagctggt cgtcggtgaa gccttgcgtg gctttcagga actgccagaa
     3601 ctcgctgcgg gcgaaattgc ccaccatgat cggccggccg cgcatgtcct gcagggtggt
     3661 gatgccctgc ccgccatggg ccactagcgt ttgcgggtcc ttctggaaga agctggccac
     3721 ggtgatcgca tcgatgccgc gcgcctgcat gttgagcgtg gtgaagctgg tgccgatgcc
     3781 caggttcatc cgccccgagc ccaccagcag cggcaggttc acatccggcc cgccgttgac
     3841 gatctccaca ttgaggccgg cggccttgta gagcccggcc ttctcggcct cgaagaagcc
     3901 gcaatgctcg gtctcgggca cccagttgag gcccacggtg atatgatcgc ccccggccgc
     3961 cccgccatcg ctgaacagcg ccgccgcgca gcccaggaag aacagggcaa tgccgccatg
     4021 aaaggccaga tcaagcgcgc gagcggcgcg ggccggccag ctcatgcgcg caccggctgg
     4081 cgaacgggcg caggggccgg cggcgcgaaa gtgccgctgg tgacgatata gcccggcaga
     4141 tggcggatgc gatccaccca gcggcggatg gcgggataat ccatgcgggc aatgccccct
     4201 tcctcgcaca gggcgatgtg gacatagcag gcgatatcgg ccgcgcaggg cccctcgccc
     4261 gccagcagcc agcgctggcc ggcgcgctcg gcaaaccaca ggtggcgctc cacctcgcgc
     4321 agcagggcat gggccgcggc gcgcaaggcg gcgccatcgg caggcacgcc cagcccctcg
     4381 tgcaggcgcg caacccccgc gctggcggcc agatcgcgcg ccatgcccag ccagccaatg
     4441 aaggccggat ccgcaccgcg ccaccagccg ggcgcatccg ccctggcggg cgtatcgccc
     4501 cgggtagcaa tggcgcacag caccgcctgc caatccgcca gcggggcgcc ggcgccttcc
     4561 agcaccggca gccggccaag cgggctcagc acgcggaagg cgtcggtttc atggtcgcgg
     4621 ccggggtgga tgtcgaggtg gcggatctcc agcgccacgc cggcaaggct ggccagcagc
     4681 cgcgcggcat aggcttcggc gagcagatcg gaggtgtgga ggatcaacat gcggcggcct
     4741 ccatcagggc agcgtcttcc aggcggcggc gggcagcttc cagcagcagg tcggccgcca
     4801 tgatatccac ccctgcggcc agcccgcgga tcaccgcgcg atcagccgcc agcggctgca
     4861 cgaacaggcg caaggccggg tgcgccgcca tcagcgctgc ggcttcctgc gccacggccg
     4921 ccgccgggcg gttcaccggc agggcgcgca gcacctcgcc cgccagatca gccggcggcg
     4981 cgccttgcgc ggcaagggcg gtccacacca gcccggccac ttcggccacc ggccagggcg
     5041 tggcatggat ggcgcggggc actttcgcat tcggctgggc ggggatgaag gtgcaggcgc
     5101 ccgagcccgc ggcaaagcgc caggcatggt agacgcagcg cagctcgccc cccaccacat
     5161 cgcccgccga aaagcgcacg ccgcgatgcg ggcagcggtc ttcccagaca ttgaccgcgc
     5221 catcatcgcc gcgccacagc gccagcccct ggccggccag catcgtctga tacacatggc
     5281 ccggcgccag atccgcgctg gccgccaccg gcacccatgg gccccgcccg ctcatgccgc
     5341 cacccttgcg ccggcgggaa tgacgccata ggccagccct tcggcgacaa gatagcggcg
     5401 ataggccacc gaggtggaat ccgccgcgat cggcagttcg gcgccatcgg ccagcggcag
     5461 gcgcctgggg gtctggtttt cgagaatggg cttgtcctgc aggaagatca gttgctggaa
     5521 gcggcgcaga tcggccggct gcgtgccatc gggggcaaac agcatcagcg tgtgggcgat
     5581 ggaacgctcc tcgctgaccg gctggacgaa gagatagatc acatcccagg catcgcgcgc
     5641 caccggattg gccttgtgga ggcaggtgat cagcgggcgc accacgcaat actgatagtc
     5701 caccatatag ccgccctggg ccgccggcga ggccaccggc tgatagacct tgcagccggt
     5761 tgccagcacg ccgccgcctt ccagcggccc cacgcgatag gggtgcacca tcgtctcggg
     5821 ctcctcgccc agatagccga catggacgta gcccagatgg cccagatcga ggaagttttc
     5881 caccacccgg ggggccgcca catgcacccc caccgaaccg ccggtggcga tggtgcgccc
     5941 ggcctcggca tattcggcaa tggtgatcag cgggcgagcg ggggcgccaa ggcacgccca
     6001 gaggaagcca taggcgacct cggtgtgcag cggcgcgcca tcggcccgcc gcgcttgcgc
     6061 cccggccgcg cccaggcggg ccagcagcgg cacgtccagc agggtggtgg caaaatccgc
     6121 gcctgcgcgc agcgcggcca gatcggcgat cacatgccag tcgttccaca acacaggatc
     6181 gcgcgcgccg cttgcagcgc cagtcaaaat ctcggccatg ttcccgcccc gatattgcaa
     6241 tgcgtcatgg atcgacgatc agggggatcg aagcaaacac agatttatgg catttgcgtt
     6301 cgaattttta taacactgcc tagccgccaa tcccccccac accgctgtcg cgcatggttt
     6361 gcgccatgtg ttgcagcagc agcgaggccg gcaccgacag cgtgcgcccc gaagcggaaa
     6421 tgagcgagag caccgagagc ggaatattcg gctcggccag cggcacgaag accagcgtgc
     6481 cggcctcctc ctcggccgag acatccagtc gggtgagcat gcccacgccc cgcccgccct
     6541 tcaccagcgc cttcagcagg ctgatgctgc cggcattcac cgccccgcgc gaacgcccgc
     6601 cgatggtgcg cgcccagatc gtgtcgagca cctggcgata ggagagcgtt tcatcgggca
     6661 gcaccagccc gtggtcggcg caatcggcaa agctcacttc ctcgcgcccg gccagcggat
     6721 ggccggcggc caccaccgcg cccagctgat agaccatcgt gcgctccaca cggaaggcct
     6781 ggccttccgg cgcgttaaag gtgaggccga tttccgcctg cccttccagc accttctcgc
     6841 ccaccaccga ggccccggca gtgacgaaat tgagatcgat gccgggatac tgatcgcgga
     6901 aaggggcgat cgcacgggca acgaaatcgg ccgcgccctc ggccatggcc acggtcacct
     6961 cgccgcggcg caggcccagc aggtcgtcga tctgcgccac cgcatggccc cattcgcggc
     7021 gccagcggcg gatataggtg atcagcactt cgccggcggc cgtcaggcgc aggccctgcg
     7081 gcatgcgttc aaacaggcgc acgcccagtt cctcttccat cagcagcatg tgccgatcga
     7141 tcgccgaggg cgaaatgcgc agcctttccg aggcgcggcg gatcgagccg gcgcgcacga
     7201 cctcgtcgaa atagagcgcg aaacgggaaa acatcgcgag cctcctgtgc cggcgggcgg
     7261 caggcgggag ggccagcgcc gaccgttcta aaaatcagat cacttttccc gatctattgt
     7321 aatggatttg aacggcagcg ccagatagat tttgctccgt cagcgaagcc gcgtccgacc
     7381 ttgtccctcc tcttccgcgg cgtggcctcg ctggcgcaac agggaatacc gatcagacaa
     7441 cagagcagat catcgaaaag ggggctatca tgagggaatt ccaaaatccg gcgcgccggc
     7501 agttgtggcg cgtggctggc ggttcgggcg tggcccgggg cgctttggtg gcggcgctgg
     7561 gctcggcgct ggcgcttgcg ggcacggccc atgcggcagg ggccaatgcg gctgatgcag
     7621 cggcggccga tgccgcgccc gtgcaggggc tgggcgatat cgtcgtctcg gcttccaagc
     7681 gcgagaccga cgtgcagaag accccgctgg cggtgaccgc gctgaccgcc gacacgctga
     7741 ccgccaacca tgtgaccgat gcggtcgacc tcaacgggct ggtgcccagc ctcgtcatca
     7801 ccacgtcgga aggctttgag cgcaacatcg ccattcgcgg cgtgggcttc aacgtgccgc
     7861 agaacgatgc cgcgcagccc tcggtgtcct accatgtgga tggcgtctac attgccaatc
     7921 cggtggcgat gaacaccaat ttcctcgatg tgaaccacct ggaagtggtg cgcgggccgc
     7981 agggcaccgt ctatggccag aactccaccg gcggcacggt caatgtcgtc accaaccagc
     8041 cgcagctggg caagttcggc ggtttcgtca agttcgacac cggcacctat gcgctggccc
     8101 agttcgaggc cgcggccaat gtgccgctgg gcgataccgt ggcgatccgc gcggcgatcc
     8161 aggcggtgca ccacgatggc tatgccgtgg ccaccaaggt gccgggcacc aatggccatt
     8221 atggcctgtc ggacgagaat tcgatgcatg gccggctcag cctgctgtgg gcgcccaccg
     8281 accgcctctc gctgatgctg cgcgccgaat atgcccatgc caacaaccac gagaccgaag
     8341 gcaagagcat ctacgacacc gatcccaacc cctggcacca gagctccgac tggccgggca
     8401 agatcgcata tgacaactcg atgttcaccg gcacgctgaa ctacgacatg ggctgggcca
     8461 agctgaaggc gctgggcagc tggcagatgg tgcgccagaa cggctcggtg aacgaggacg
     8521 ggctggatct ggccggcgcc attgccgcct atggcgtcac cggcttcgac agcgtgcacg
     8581 acatcgaata tctctaccac cgctccaccg cgaaaacggg cgagatcgac ctcgcctcgc
     8641 cggccaacca gatgctggaa tggatcgtcg gcggcttcta cctgtcgtcg aactatcgcg
     8701 tctcttacga tcagtatgcg gtggacagct atccggtctc gggccagccc acctcgctgc
     8761 agaaccagat cttccccgcc gtcaccaatg gcgacaacct gggcttcgag ggtgctgacg
     8821 gcaatttcta ttacccctat ttctattcga tctcctatct cacgcgcgaa tcctattcgc
     8881 tctatggcca ggccacgctg catctgagca gccgcctgcg cctgtcgggc ggcgtgcgct
     8941 acacgcatga ctacaattcc accagcatct gcgactatgc ctgcgacacg cccaccttcg
     9001 tgcagcagag cagcaatgcc tggaccggca aggccggcgt ggaatatgat ctggcccgca
     9061 agaccatggc ctatttcacc tggtccaccg gcttcaagcc gggcggcggc aacatcagcc
     9121 ccacgcccat cgtgctgggc tataccttca agcccgaaac cgtgaccgcc tacgaactgg
     9181 gcgccaaatc gaccatgctg ggcgggcgcc tgcgcctcaa cgtcgccggc ttctactacg
     9241 actacagcaa cacccagttc gaggcggaag atgccgtggc ctatcagggc ggcgttgcca
     9301 atatccccaa gctgcacatc tatggcgtgg aagcggaagc ctcggcgctg ctgggcggcg
     9361 gctggcgctt cgatggcaat gtgacgatcg aggacagcaa gatcacctcg cattacatgg
     9421 ccctcgacaa tgtcaccggc tacaacatcg acaagcagat cttcgcctcg ggcaattcgg
     9481 tgttctatga tccgggtcgc tcggccgata tcgccgcgcg cacggcggcc tatttcgacg
     9541 tgtatggcaa ccaggccccc aacctgccgc acgtcagcgc caccggcacg ctgagccaga
     9601 aatccgacct gccctggggc ggtgcgctga ccagcagcct gcagggcgaa tatcgctcca
     9661 gctatgtgaa cgcggtctat ggcgacatgg tgatcaatgg cgtccacgtc tatcgctcgc
     9721 cggcctacaa cctgtggaat gtgaacttca cctggcagcc ggtgaacccg catttcaaat
     9781 tctccgcctc gctgaagaac atcttcaatt cgggcgcggt gctgggccag ttcaccaacc
     9841 agttcggcgg cgagatcact cgccaatatg cgccgccgcg ccggctgatc gtctctgccg
     9901 cgtataattt ctgagccttt cccccggtcc accgcggcac cagctcccgc cgcggtggac
     9961 ctcccccgcc aacccctgca tgaagtgctc cacctttcac aacacaggct ctcccgcatg
    10021 acgatcgccc gcccctccct ttccggcccg cgcccccacc ggctgttcga catggccgcg
    10081 cgcggcaccc gcctgcccac cgagatcctg gccggctgca ccaccttctt caccatggcc
    10141 tatatcatcg tggtgaaccc ctcgatcctg gggctggctg gcctgccggt gccttctgtg
    10201 gcgatggcca cctgcctgtc ggcggggctg gcctgcatcc tcatggggct gatcgccgat
    10261 gtgccgctgg cgctggcgcc gggcatgggc ctcaacgcct atttcagctt caccgtggtg
    10321 cagcagatgc atgtgccatg gcaggtgggg ctgggctgcg tgttcctgtc gggcctggcc
    10381 tttctggtgc tcaccgcggc cggcatccgc cagatgatcg tggcggccat tccgcattat
    10441 ctgctggcgg cggtggcggc gggcatcggc ctgttcatcg cctttgtcgg catgaagaac
    10501 gccggcctta tcgtggccag cccggccacc attgtggcac tgggcaacct gcgggaaggc
    10561 ggcgcgctgc tggcggtcat cggcctggtg ctgatcaccg tgctcagcct gctgcgggtg
    10621 cgcgcggcaa tcctcatcgg catcatcgcc accacgctga tcggctggct gaccggcctt
    10681 gccaccgcgc ccgccaccgc gccctcgccc gccggccttg gcgccacctt cctcgcgctc
    10741 gacattcccg gcgcgctgca tatcggcagc aagggggcgg gcattgtcgg gctggggctg
    10801 gtggagatcg tcttcgtctt cctcttcgtc gatctgttcg acaatatcgg cacgctggtg
    10861 gccgtaacca agcgggccgg gctgatggag gccgatggca ccatccccgc gctcaaccgc
    10921 atgctttatg ccgatgcgct ggcaacgctg gccggcgccc tgctgggcac cagcaccgtc
    10981 acgtcctatg tggagagcac ggcgggcgtg gaaagcggcg ggcgcaccgg cattgccgcc
    11041 atcgtcaccg gcctgttgtt cctgctggcg ctgcctttgg cgcgctatgc ccagctggtg
    11101 ccgctctcgg ccacggcccc ggcgctgatc ctggtgggcg cgctgatgat ggcgccgctg
    11161 accgaaatcg cctgggagaa ggccgaggaa gccgtgcccg ccttcctgac gctggcgatg
    11221 atcccgctca ccttctccat cgccaatggc ctggcctttg gcatcaccgc ccatgcgctg
    11281 gtgaagatca tcaagcgcga agtggccgcc agggattggc tgctgttcgt gctggcgctg
    11341 ctgttcgtca tccgtttcgc ctatatttct gccgcctgag gaccaagaat gcccacattg
    11401 tccgcgtctt ccgccaaggg gctggttgtt gccccccatt tcgccgccgc cgaggccggg
    11461 cgggccgtgc tggccgccgg tggcaatgcc gtggaagcgg cggtggcagt ggctgccacc
    11521 atcgccgtcg tctatccgca catgaactcg atcggcggcg acggcttctg gctgatccac
    11581 cgccccggcc agccgatgac cggcgtggac gcctgcgggc gcgcggcagc ggcagctgac
    11641 cttgccctct atgctggcca tgccacggtg ccctggcgcg ggcccctggc ggccaacacc
    11701 gtggccggcg ccctttccgg ctgggccagg gcgctggaga ttgccggcgg cagcctgccg
    11761 ctggaaagcc tgctggccac cgccatcgcc catgccgaag agggcattgc cgtcaccgct
    11821 ggcggcgctg ccaatgcggc ggccaaaagc gccgagctga ccgcctgccc cggcgcctat
    11881 gccgccatct tcgagcccga tggccggccc ctgcgcgaag gcgagatcct gcgccagccg
    11941 gccctggcgc aaaccttgcg ccggctggcg gcagaggggc tggacagttt ctatcgcggc
    12001 gcgctggcgg ccgatatcgc cgcggatctg gccgcgctgg gcagccccgt ttctgcggcg
    12061 gatctggcag cgcacgtggc cgaggaaagc ctgccgctcg cctcgcgcat tgccggcgcc
    12121 acgctctaca accaccgccc gcccacgcag ggcatcgcct cgctgctcat cctcaccctg
    12181 gcagaccggc tggccaaggg cagcgagggc agcttcaccc atgtccacgc cctggtggag
    12241 gccaccaagc aggccttcct gtggcgcgac cgccattgcg gcgacccggc ctatatggac
    12301 gaggccgcgc aggacctgct cgacgatgag ccgcgcatcg ccgccatggc cgccgcgatc
    12361 gacccggcgc gggcgctgcc ctggccccag cccagccagc agggcgatac cgtgtggttt
    12421 ggcgcggccg acaaggacgg catggtcgtc tcgctgatcc agtccaccta tttcgaattc
    12481 ggctctggcc tggtgctgcc ccaaaccggc atcacctggc agaaccgcgg cgcctccttc
    12541 cgcctggccg aaagcgggtg gaacgcgctg aagcccggcc gcaagccctt ccacacgctg
    12601 aacccggcgc tggcccgctt cgacgacggg cgggtgatgg cctatggcac gatgggcggc
    12661 gagggccagc cgcaaaccca ggccgccgtc ttcagccgcc atgtgtggca tggcctgccg
    12721 ctggcagacg cgattgccgc gccccgctgg ctgctgggcc gcacctgggg cgaggacagc
    12781 accagcctca agctggagga aggctttgcg cccgagctct atgcccagct ggccgcggcc
    12841 ggccatcagg tggagatcct gcccgcctgc aattccacca tgggccatgc cggcgccgtg
    12901 ctgcgccacc ccgacgggct gatcgagggc gccagcgatc cgcgctgcga cgggatggcc
    12961 gcggcatgct gagcgcggcc catcacgccg ccggcgcccg cgccacccag cgctgccggg
    13021 cgctgggcat cgcgccctac agcgaccttg ccgatgcgct ctaccgcgcc tggctgaccc
    13081 cggcccacaa ggcggcgctg ggcgcgctgg aaggctggat gacagaggcc ggcatggccg
    13141 tgcgcatcga tgccatgggc aattgcatcg gccgctatga aggcaccgcc cccgatgcgc
    13201 ccaccctcct catcgccagc catatcgaca gcgtgaagaa cgccggctgt ttcgacgggc
    13261 cgctgggcgt gatgctgggc gtggaatgcg tgagcgcgct gcatgccgcg aggcgccgcc
    13321 tgccctttgc catcgaggtg gccgcctttg gcgatgagga aggcagccgc tttcccgccg
    13381 ccatgctgac cagtcgcgcc atggccggca cgctggacac agcagacctt gccattgccg
    13441 atgcgcaggg caccagcgtg gccgaggcgc tggccacttt cggcctcgat atcgcccgcg
    13501 cggccgaggc ccgcctgccc tacacgccga tcgcctatct ggaagcgcat atcgagcagg
    13561 gcccggtgct ggaagccgaa gggctggcgc tgggcaccgt taccggcatt gccgcgcaat
    13621 tgcgctatga agtgcaggtg gaaggccagg ccggccatgc cggcaccacc gccatgccgc
    13681 tgcgccgcga tgcgctggcg ggcgctgccg aaatgatcgc cgcgatcgag gagatcgccc
    13741 gcgcggccgc cagcgatgtg gtggccaccg tcggccggat cgaggcgcgc ccgggggccg
    13801 ccaatgtcat ccccggcgcg gtgcgcttca ccattgatgt gcgctcgggc gcagaaggcc
    13861 ggcgcgatgc cgccgccgcc ggcatcctgg ccgccatcgg cgcgattgcc ggccggcggg
    13921 ggctggccgc gcggtgggaa gagattaaca acctgcccgc ctccccttgc gcagagccgc
    13981 tgatggcgct gatggacagc gccatcgcca gcaccggcca gcgccccttc cgcctggttt
    14041 cgggcgccgg ccatgatgcg atggtgatgg cggccctgtg ccctgtggcc atgttgttca
    14101 tccgctgcga gaagggcatc agccacaatc cggccgaaaa cgtggaagtg gcggatgttg
    14161 ccgccggctt tgccgcgatg atggccttta tcgattccct gggagacacg ctttgactga
    14221 taccttgccg caggacctgt tcggcgagat cgatccgccc cagcgcctgt tgatgggccc
    14281 gggcccggtg aatgcccacc cgcgcgtgct gcgcgcgatg agcgccgacc tgctgggcca
    14341 gttcgacccg gaaatgaccg gctacatgaa tgaggtgatg gcgctgtacc ggcccatctt
    14401 cggcaccgcc aaccgctgga cgatgctggt ggatggcacc gcgcgcgccg gcatcgaggc
    14461 cagcctggtc agcctggtgc agccgggcga tacggtgctg gtggtcaatt tcgggcgctt
    14521 tggcctgctg ctgcaggaaa tcctcacccg catcggcgcg cgcattgcca cggtggatgc
    14581 gccctggggc gaggtggtgc cgctggaagc gatccgcgcg gcagcgcaga aggtggggcc
    14641 caaggtcatc gccaccgttc atggcgacac ttccaccacc atggcccagc cgctcgacgg
    14701 cattggcgcc atcgcgcggg aagtgggcgc gctggtctat gtggatgcca cggccaccat
    14761 cggcggcatg gaactggcgg ccgaccgctg gggcgccgat atcctcaccg gcggcctgca
    14821 gaagtgcctg ggcggcccct cgggctcctc gccgatcacc gtttcggatg ccgccgccgc
    14881 ccacatcttc acccgccgcc acatcgagaa gggcatccgc gccgccgatg ccgaggatgg
    14941 cgagggggcg atgatcggct ccaactattt cgatctggcg atgatcatgg attactggag
    15001 cgagaagcgc ctcaaccacc acaccgaggc gaccaccatg ctttatggcg cgcgcgaatg
    15061 cgcgcgcgtg gcgctggccg aggggctggc ggcgcgctat gcccgccacc gcgcggcggg
    15121 cgctgccatg agcgcgggcc tccaggcgat gggcctcacc ctgtttggcg atgatgccca
    15181 ccgcatgacc aatgtcaccg gcgtctacat ccccgccggc gtcgatggcg aggcgatccg
    15241 caccgccatg cgccgccatt tcgagatcga ggtgggcacc gcctttggcc cgctggtggg
    15301 caagatctgg cggctggggg cgatgggcta caacgccatg aagcacaagg tgctgatcac
    15361 cctggccgcg ctggaagcct gccttgccgc cgagggcttc agcctgcccc ggggcgcggc
    15421 ggtgccggca gcgctggccg cctgggaggc cagccggtga gcacgcccaa cactaccccg
    15481 cgcgatctgg tgggctatgg cgccacgccg ccggccgccc aatggcccga tggcgcgcgc
    15541 atcgctgtca atttcgtcat caactatgag gagggcgcgg aaaactgcgt tctcaatggt
    15601 gatgaactgt cggaaggctt cctttccgac atggtgggcg ccgcccgcca ccccgatcgt
    15661 gcgatggcga tggaaagcct gtatgaatat ggcagccggg ccgggttctg gcggctgcac
    15721 cggctgttca ccgggcgcgg cctgccggtc accgtgttcg gcgtggccag ggccctggcg
    15781 atgaacccgg cggccgtcgc cgccatgcag gccgccgact gggagatcgc cagccatggc
    15841 ctgcgctgga tcgactatca atataccccc gaggaagtgg agcgcgccca tatcgccgag
    15901 gcgatcgcgc tgcacacgca agtgaccggc agccggccgc agggctggta tcaggggcgc
    15961 accagcccca acaccgcgcg ccttgtcgcc gaggaaggcg gctttctcta cgatgccgat
    16021 tcctatgccg atgatctgcc ctattgggat gccagccacg gccgcgccca gctgatcgta
    16081 ccctacagcc tggatgccaa cgacatgaag atggtcgcgc tcaacggctt taccgagggc
    16141 gagcagttct tccattacct gcgcgacaca ttcgactacc tccacgccga aggcgggcgg
    16201 atgatgagcg tgggcctgca tggccgcatc gttggccggc cggcgcgcgc ggccgccctt
    16261 gcccgcttcc tcgaccatat cgccaccaaa cagggcgtgt gggtggcccg gcgctgcgat
    16321 atcgcccgcc actgggccgc cacccacccc gccccggagc ttgcctgatg atcctcaacg
    16381 accccaccct gctggccgaa atgcaggccg cctttgccga atacgaacgc gccctgatgg
    16441 ccgacgacgt gccggcgatg gatgccctgt tccacgatgc ccccaccacc aaccgcttcg
    16501 gcgtgggcga ggtgctgtgg ggcgccgagg cgatccgcgc cttccgcaag ggccgtggcg
    16561 gctcgcccca gcggcggctg ggccatctgg cgatcaccac ctattccacc cccgatggcg
    16621 ccgcctttgc caccgccgat gtggagttct tccgcgagaa cagcgagcgg cgcgggcgcc
    16681 agagccaggc ttgggtgaaa ttcgccgatg gctggaaggt ggtttccgcc catgtcagcc
    16741 tggaagggaa cagccactga tgccggtgga tttcaacgcc ctgtctgacg ccgatttcat
    16801 cgcccgctat ggcgcgctgt tcgaacattc gccctgggtg gtggaacgcg ccgcccgcct
    16861 gcgccccttt gccgatctcc acgaagggct gatgcgcgcg gtgcatgaag ccagccggga
    16921 cgaacagctg gccctcatcc gcgcccaccc cgagctggcc ggcaaggccg ccatcgccaa
    16981 ggagttgacc gatgcctcca ccgccgagca ggcctcggcc gggctcgacc ggctgacggc
    17041 cgaggaatat gcccgcttcc atgcgctgaa cgcggcctac agggccgccc atgccatgcc
    17101 tttcatcatc tgcgtgcgcc tgaccgacaa ggccggcatc ctggccgcca tggccgcgcg
    17161 caccgccctt gccacagacg acgagatcgc caccgccatc gccgagatcg gcaagatcgt
    17221 tgccctgcgc cttgaggacc ttgcatgacc ctcctttccc atatcgacct tctggaatgg
    17281 ctgaatctgg cgctgcgctg gctgcatgtc atcaccggca tcgcctggat cggcgcgtcc
    17341 ttctatttcg tctggctgga taaccacctg aagccgccgg ctggcggcgc ggtgccgggc
    17401 ggcccctcgg gcgagctgtg gtcggtccat ggcggcggtt tctatcacaa ccagaaatac
    17461 cccgtcgccc cgccccggat gcccgacgag ctgcactggt tcaaatggga ggcctatttc
    17521 acctggatga gcggcttctc gctgctggtg ctggtctatt atgtgggggc gaaaagcttc
    17581 ctcatcgatc cggccaaggt ggcgctcagc cagccgcagg cgatcggcat tggcctggcg
    17641 ttcctggcgg gcggctggct ggtctatgac gggctgtgcc gcacgctggc gcgctcgccc
    17701 cggctgctgg gggcgatctg gtttgccgtg ctggtggggg cggcctatgc gctcaacctg
    17761 ctgttcaacg cgcgcggggc ctatctgcat gtgggcgcga tgatcggcac ggtgatggcg
    17821 gccaatgtgt tcctcatcat cattcccaac cagcgcaagg tggtggcagc gctggtggcc
    17881 ggcgaaacgc ccgatccggc gctaggcgcg gcgggcaagc tgcgctctgt gcacaacaac
    17941 tacatgacgc tgccggtgct gttcatcatg atcagccacc actatccgat gacctatggc
    18001 gccgatcggc cgtggctggt gctggcgctg ctgggggcca cgggcgttgc catccgccag
    18061 gtgttcaccc tgcgccacaa gcatgccccc accggccgct ggatcgcgct ggccgtggtg
    18121 ctggcgctgg ccagcgtgac ctatgtggcc gccgaaaagg ccagccacga ggaaggcacc
    18181 gccggcaatg tgacctatgc cgatatccag ccgatcctcg ccagacattg ctatgcctgc
    18241 catggcgccc atccgacgaa cccggccttc ccctcgccgc cgcttggggt taagctggat
    18301 acggccgaac atgtcgccgc tgttgcgccg cgcatcaagg cggtggcggt ggaggccgta
    18361 gtgatgccgc tgggcaattc caccggcatg accaaggccg aacgcgcaca gatcggcgcc
    18421 tggatcgcgg caggagcacg cccatgagca gcctgtccac ccatgttctc gataccgcca
    18481 gcggccagcc ggcggccggc ctcagggttg tgctcagcgc cggcggcaca gtgctggccg
    18541 aggggctgac cgatgccgac gggcgcttca ggggctttcc cccgctggcg gcgggcgcct
    18601 atgtgctcac cttccacgcc ggcgattatt tccgcgcggc cggcgtggcg ctggccgatc
    18661 cgcccttcct cgatatcgtg ccgatccatt tcggcgccgg ggcggaaggg cattaccatg
    18721 tgccgctgct gatcgccccc tatgccttct ccacctatcg gggcagctga gatgggcgag
    18781 atccgcttcc tgctgaatgg cacacagcgc gccatcgccg atgccaaccc caccgccacc
    18841 ctgctcgact ggctgcgcta tgacgcgcgg ctgacgggca gcaaggaagg ctgcgcggaa
    18901 ggcgattgcg gcgcctgcac ggtgctggtg ggcgaacgga cgcagggtga cgcggtgcag
    18961 tggcgggcgg tgaatgcctg catccagttc ctgccgatgc tggatggcaa ggcggtgctc
    19021 actgtggagg ggctggccga agggggcgcg ctggcgccgg tgcaggcagc gctggtggcc
    19081 catcatggca gccagtgcgg cttttgcaca ccgggcatcg tgatgagcct ggccgccaag
    19141 gcctttggcg cgctgggcac agaacaggcc agcaccggcg atgtgctgca gggcaacctg
    19201 tgccgctgca ccggctatgg ccccattctg gcggcaggcg atgggctggc ggcggccgcc
    19261 gcgcccgatg tggcggcaca gcttgccgcc cttgcccg
//
LOCUS       DASRYE010000041        54138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_143679,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000041 DASRYE010000000
VERSION     DASRYE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 54138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54138
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143679"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="hisD"
                     /locus_tag="VFF98_02255"
     CDS             <1..903
                     /gene="hisD"
                     /locus_tag="VFF98_02255"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZU62479.1"
                     /translation="PGGRAAYPSSLLMNAIPAKVAGVGRLVVTTPTPRGQVNPLVLAA
                     AHIAGVDEIWRVGGAQAVGALAYGTARIRPVDVITGPGNAFVAEAKRQLYGVVGIDMV
                     AGPSEILVIADGRNDPRWIAVDLLSQAEHDPAAQSILITDDAGFADAVADAVEAELKL
                     LATEAAARASWETHGVIITVADLAAEAPALANALAAEHVEVATDTPQALFDEIRHAGS
                     VFLGRHTPEAVGDYVAGPNHVLPTGRRARFSSGLSVLDFLKRTSFIQLDDAALAAIGP
                     AAVALADAEGLPAHGRSISVRLGQ"
     gene            916..1353
                     /gene="nusB"
                     /locus_tag="VFF98_02260"
     CDS             916..1353
                     /gene="nusB"
                     /locus_tag="VFF98_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZU62480.1"
                     /translation="MNPRARAAARLAAVQALYQHDMEGTPLASLLDEFHRHRLGAEIE
                     DVQYNEAETAFFDDVVKGFLARREEIDGKLAEKLATGWKLERLDKTMLQVLRAGAYEL
                     IARADVPTASVISEYVDVAHAFFDEREAKFVNGVLDALAKAVR"
     gene            1361..2248
                     /gene="thiL"
                     /locus_tag="VFF98_02265"
     CDS             1361..2248
                     /gene="thiL"
                     /locus_tag="VFF98_02265"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZU62481.1"
                     /translation="MTSEAAFIEALRGLVSTPSARALLDDCAVLPLGTETLVLTHDMI
                     AAGVHFLPDADPADIAWRLVATNMSDLAAKGAEPVGVLLGHMLDEDDTRFLAGLGEAL
                     AHYHAPLLGGDTIAVPGGTRVLGLTAIGRATCPRVPSRTGARAGDGLYITGPVGAAML
                     GLEAIRAGTPDPAASLAYRRPEALMAEGRALAPLVTAMMDVSDGLLLDAARMARASGI
                     TLAIDSAAVPIATPENRRAEALRWGDDYQLLFTAPAGVVLPVPAFAIGRALDAGPAPL
                     LVDGAPAGEEAPLGYQHRG"
     gene            2442..4535
                     /locus_tag="VFF98_02270"
     CDS             2442..4535
                     /locus_tag="VFF98_02270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZU62482.1"
                     /translation="MTLVILAIGLGLLAIVYGFITSRQVLGAPSGNARMQEIAAAIQE
                     GAQAYLKRQYTTIAIAGIVVAAIVGAALGLISATGFVLGALLSGAAGFIGMNVSVRAN
                     VRTAAAAQNGLQAGLTLAFRAGAITGLLVAGLALLAISGYFYYLVAVAGAAPGSRVVI
                     TALTALALGASLVSIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYVVTVGATMVLTALLLKGADALLLPLMSLPLLIGGVCIVTSI
                     IGTYFVRLGRGQNIMGAMYKGFLVTAGLSVVAIWLAISAALGGDMESPIGANAFNGRM
                     LFYCALVGLVITGLIVWITEYYTGTGFRPVRSIARASVTGHGTNVIQGLAISLEATAL
                     PTLVIVAGIIIAYKLAGLIGIAYGATAMLALAGMVVALDAYGPVTDNAGGIAEMAGLD
                     DAVREKTDALDAVGNTTKAVTKGYAIGSAGLAALVLFAAYTTDLREFFPGLSVNFSLE
                     NPYVIVGLLLGALLPYLFGALGMTAVGRAAGDVVVDVRDQFAANPGIMAGTSRPDYAR
                     TVDLVTKAAIKEMVAPSMLPVFAPILVYFLISQVAGRDNGFAALGALLLGVIVGGLFV
                     ALSMTSGGGAWDNAKKYIEDGHYGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPMIKI
                     TNIVALLLLAALAGG"
     gene            complement(4549..6489)
                     /gene="uvrC"
                     /locus_tag="VFF98_02275"
     CDS             complement(4549..6489)
                     /gene="uvrC"
                     /locus_tag="VFF98_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZU62483.1"
                     /translation="MSKPTDPPRDPDSAERFNEDAATSLVRGREKPDLDAGVAAIREV
                     LATLPARPGVYRMQDARGDVLYVGKARALRNRVANYTQVDRLPLRLQRMVSQCRAMTI
                     VTTNSEAEALLLEAQLIKRFRPPYNVLLRDDKSFPFILLRTDHAFPRIQKHRGARRSA
                     GHYYGPFASAGSVNTTINALQKLFLLRSCTDSFMSRRDRPCLLYQIKRCAAPCVERIS
                     AADYAELVRQAKDFLGGKSSAVQRELEAQMAAAAEALDFERAALLRDRLRAATFIQGS
                     QAINAEGLPNADIFALAAKAGQIAIQAFFIRGGQNWGHRAFFPAHTDGLEEGEVLQSF
                     LAQFYEEVPPPRVILLDRALPEAELLAEALREGAGGKVEIATPQRGDRKRLVEQAARN
                     ATEALERRMAESGSKAKIWREMAEFLELPEAPQRVEVYDNSHIQGAHALGAMIVAGPE
                     GFQKGQYRKWNVKEADSNDDFAMMAEMMTRRFGRAMEEDPDRDRGMWPDLVLIDGGKG
                     QMSAVKAALEELGVEDVNLIAIAKGPHHGREGREVFHFPDGREKMLPVNAPVLFLLQR
                     LRDEVHRFVIGAHREKRSRAITASPLDEIPGIGPARKRALLLHFGTASRVRAAALDDL
                     MRAPGVSAAVARTVYDFYHPGG"
     gene            complement(6583..8007)
                     /locus_tag="VFF98_02280"
     CDS             complement(6583..8007)
                     /locus_tag="VFF98_02280"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZU62484.1"
                     /translation="MTATEPDTQPAASHNAPRASGGRRVWRKVALGAAALAVAGGATL
                     GWFLAFPAQAPAAMGRWIEDTTGANPHPVKLLRPAEKPLSVLAQVGQKLFFDPSLSAS
                     GKQSCASCHDPARAFGPPTARDVELGGIAMDQEGLRPPPGLTYLYRQKGFTIGPSTAD
                     LDVPIPLDQLADKAQTQARQVKSAGTPAEASALVPAGGLFWDGRIDMLMDQAMGPMMN
                     PAEMANKDRFAVVDKLRRLPYLAQLGRLFGQHTLDRPEMLFIEAMSALSAYQVESGAF
                     HRFNSKYDAWLEGKERLTHAEMRGLRLFNDPEKGNCAACHLSQPAADGLPPLFTDSEF
                     EALGVPRNPRLKVNRDPAFFDMGLCGPARQDLARQTQYCGMFLTPTLRNSARRGVYFH
                     NGVYHNLRDVLNFYNQRAAHPEKVYPRDAQGRLLIYNDLPAKYHGNIDVADAPFTQKR
                     GDTPPLSDADIDDIIAFLHTLDDR"
     gene            complement(8086..9756)
                     /locus_tag="VFF98_02285"
     CDS             complement(8086..9756)
                     /locus_tag="VFF98_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU62485.1"
                     /translation="MTATAGAALLLAGCHDDNKNTSTDSNTTAQDKLTTTTPIKHLVV
                     IFPENQSFDHYFGTYPQSADPTGEPTQSWTAPSNVAYVNNYLKHPSLLNANPNMTNSL
                     NTSLGYQASYLNPFRIDRAQYNVTSMNHAYLPEQEASDNGLMDAFVYYTGKAATSGSA
                     QFGTKAQVLGYFDGNTVTALWNYAQNYAMSDNAWTDTFGPSTPGAINVISGQNNGAVT
                     STGSAIASSAIADGNGSFSVIGDPDPTGDKCSSTTSATVKMSGQNIGDLLNAAGITWG
                     GFMGGFNLATTNSNNTTGCARSTVSPNTSSTVTDYIPHHAWFQYYGTTANINHTRPSS
                     TAMIGYTETTDSSYGATAINHQYDYNDFVTAVSAGNFPSVSFIKAPAFQDGHPGNSNP
                     LDEQTFITTAINFIMQQSDWKNTAIIIAYDDSDGWYDHQSPTITTSSSDATYDAFTAA
                     GSCTSSSATAGVGLSGKTVNGRCGPGTRTPFLVISPYARQNYVDSTQISQSSIVRFIE
                     DNWLGGKRLGNGSNDAAAGDIRSLFDFTSSPRTGTYLLNTTTGVRTTS"
     gene            10179..10802
                     /locus_tag="VFF98_02290"
     CDS             10179..10802
                     /locus_tag="VFF98_02290"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZU62486.1"
                     /translation="MPIAAPRAHGQVIEIGDDGVVRQRSNTAAVGWTTLPAPGARPAP
                     RAGHGASALPAASVLPAATSLPAGSVEAAGVPEIWRAPFAAAASRYNVSPHLLSALIW
                     QESRWHPQAISPKGAVGLGQLMPATARALAVDPRDPEANIDGAARYLRLMLDTFDGDV
                     VRALAAYNAGAARVARAGGVPAIAETRSYVANILNRVTATTTTGLLK"
     gene            10799..11134
                     /locus_tag="VFF98_02295"
     CDS             10799..11134
                     /locus_tag="VFF98_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZU62487.1"
                     /translation="MIKRFALRARLAALSAASSLLASSPALAQAAGGTDPAGSGPIVA
                     AMGWLQGTLLGNVATAVAVIAVAMVGFMMLTGRMNWRFGATVIIGCFILFGASAIVSG
                     IQATAQATH"
     gene            11151..11426
                     /locus_tag="VFF98_02300"
     CDS             11151..11426
                     /locus_tag="VFF98_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB3"
                     /protein_id="HZU62488.1"
                     /translation="MNRAPVFRALTRPQMFGGVTYSFFVINMAVTTEAFLISHSFWAL
                     PLALVVHGVGYVACLREPRVFDLWLTRVSRCPRVRNWARWGCNSYAP"
     gene            11423..13876
                     /locus_tag="VFF98_02305"
     CDS             11423..13876
                     /locus_tag="VFF98_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HZU62489.1"
                     /translation="MSGGKTASRAKAPAWKGPAAWGPREARAGDRLPYAELADGTTLR
                     LRDGSLMAMIQVPGLPFETEDDEALDHHLGVRETMLRSALDARFVVYHHVLRRRVEVS
                     LDTPGHGAPADPFAALLHSRWQDRLSTRRLFVNEQVLTVLRRPARGKTGWPEKIRRRL
                     AGTSAGPDPADRRALDAAVTALVAALEPYGARVLGSYAGPRGALFSEPLEILSALYNG
                     EMRPVAVPPQGTDIGHHLPYARVSFGLDAIEVAQPSGRSFAAMLAVKDYPDSTRAGLI
                     DNILRLPHELVLTESFAPADRQIARERIDLAIRRLRSADEEAGAERREMLAARDALGA
                     GQVGFGDHHLSLMVRAGDLPALDRAAAAAGAALADLGAVAVREDVNTEPAFWGQFPGN
                     ESYVVRRALISTANAAGFLSLHGMPFGRPEGNHWGPAATVFETTSATPYFFNFHEGDL
                     GNFTVIGPSGSGKTVVLNFLAAQAQKFAPRTVFFDKDRGAEIFLRAIGGHYARIQPGE
                     PTGFNPLAMPDTALNRAFLRDWLGVMLAAEGPEELASIAQAVDACMEAEPAFRRLRHF
                     RELLGGARRPEPGDLPSRLDPWINTGEHAWLFDNAHDALDLDARTLGFDMTALLDTPA
                     LRTPAMLYLFHRIDERLDGTPTMVLIDEGWKALDDPVFAARIRDWLKTLRKRNALVGF
                     ATQSARDALDSRIASAIVEQTATMIFTPNPRARPEDYCEGFGLSAHELELVRQLPAHS
                     RCFLIRHANHSVVTRLDLGGEADILTVLSGREASVRRLDGLRARLGDAPAAWFEALTG
                     TPWPGRADVQAPPLPSAAE"
     gene            13873..15090
                     /locus_tag="VFF98_02310"
     CDS             13873..15090
                     /locus_tag="VFF98_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZU62490.1"
                     /translation="MNACPAMQLDGASLAQTLRGLDCAMGAGTATAFSRLFGSHGALM
                     PALTGLLTLYVGFFGWGLLTGRTRLGLSALTPRMLTIGLVMTFATSWMAYQNVVWQLA
                     VGGPDQIANVIAGGHGSAMNAFADKIEIIFSAIADATHQSAAGAVAASQATADGASRT
                     VSFLSPQGMLWISGLMLLVGTVGVMVTAKIVLAALLTLGPVFIVMALFPATRGLFEGW
                     LKASVASALVPLLTVLVGGAMLSMIAPLIRDALNDSTEQATRGAMMLFLSACVYVALM
                     VLLGRTAAMIVSGWRLPLGQPPAPATVVAAAAAATAAPAITAGIAPSFAQAANPRVRA
                     MMAGLQASAREAADSAGTSAERTERARTLVMGLAPVAASAAASPGDRRLQGVGSRFRP
                     RPDASTPSAKAHP"
     gene            15087..16007
                     /locus_tag="VFF98_02315"
     CDS             15087..16007
                     /locus_tag="VFF98_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HZU62491.1"
                     /translation="MKLDPLSSSALALLAAALPVLAPAASARHARHDARLVPAAGAHD
                     ARIVGRLYDPGVIVTVRGHTGIQTTIEFRADERIDNIAVGDSTEWQVTPNKRASLVFV
                     KPMKASSHTNMTVITDRRTYLFDLVARPGLAPAYILRFSYAPDPVPPPPPTLLPVEAE
                     IARAGSPPPESSPADLHFNWVMSGMRSLWPARLFDDGQSVWLSWPRDAEPPAILRHDP
                     AGGDGPLNYRIAGDYIVVDGVPAQITLKRGKQTATLMAPARLVPGSDAPAGKTPRHHK
                     PQGTVAAPPPAAEAGKDSAAPAAASTGPHP"
     gene            16004..17116
                     /locus_tag="VFF98_02320"
     CDS             16004..17116
                     /locus_tag="VFF98_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HZU62492.1"
                     /translation="MSPAPTPPNRPLPNRPLPSPAGEGDPRLKMDDEALAMASRNAFP
                     VVAQARRRSDTMGLALGALVAVLLGAVTLVSLSRHRTAPAADRLATALPTPAPSATPH
                     PGFTPIALPLPVTPAKAMAGISAVAIAGAASPAMVYDGPSGPAVAAPAAAPAAAHGGE
                     GEGKDKSEDTDQFLTKANSAVDTATAEKMGDPANTITQGTLISAVLETALDSDLPGYV
                     RALVTENVRSFDGSRVLIPRYSRLIGQYKSGLATGQQRAYVVWTRLLRPDGVSVALGS
                     PGIDFGGQSGLTGKVYSHYLQRYGPSVVLSVLTGLATQSGNSVVVSTGNTASSVAAQH
                     DTQIPPTIKVAAGSPIRVFMARDLDFSSVTGAGRKP"
     gene            17113..18093
                     /locus_tag="VFF98_02325"
     CDS             17113..18093
                     /locus_tag="VFF98_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZU62493.1"
                     /translation="MNLPASDTASPPGGVYLNAYLAPLEQWLAREDVTEILVNRPGEV
                     WIEAAGQGMLRHAAPAIDDRHLQRLAEQVARVSHQGINRERPLLGAVLPDGARVQFVA
                     PSATRAHWAMAIRRHRMVELPLSAYDHGPLPPPPTLAGVPDPAADPVGFLHHAVQAKW
                     TVLVSGGTSSGKTTFLNALLGAVPAEERVVVIEDTPEIRLRHANSLGLVAVKGEMGEA
                     KVSTDDLLQASLRLRPDRIVVGELRGRETVSFLRAINTGHPGSFSTIHASSPAGALEQ
                     LALMTMQAGLGLSRHDTLEYARTMIDVIVQLDRAQGQRGIRAIEWLRRPD"
     gene            complement(18139..20775)
                     /locus_tag="VFF98_02330"
     CDS             complement(18139..20775)
                     /locus_tag="VFF98_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62494.1"
                     /translation="MRRPYHLSVSSVLSVLLLPGAALAQTQGSSASAADTASSADTTS
                     SADTTSSADSSAQGGGKRPIVVVAGKIAGQVDAPQAPIVTFDEEDIASYGVSSISDLI
                     DAISAETNSGRGRSSDGPIILVNGQRIASFRELRDYPPEAIRKIEVLPEEVALRYGYA
                     PDQRVVNIILKEHYRASSVEAEYTQPDAGGTSTVKGSARYAKIDKNRRLNIAASVDHT
                     TPMTEATRHVAEAASSQSSVATDPSQADNRTLVSDTRDYKLNATYTIPIGSGPTGGSL
                     AMNGALTRTETDAKNGLNTLTITGAGAGGADVVRTFPGALGTFTRTDEIQAGTSFNKN
                     VGGKWQLSATVDGSHTDAVTRTDDRADTSGITLADPYGTLPALPYAGLAVASTRTDTL
                     TSLVTLMGRPVHGPAGDIALTVKAGFAYTWQEATSTGNTNAANNTSTRFRRGDASTGF
                     NLAIPLTSTREHFGEALGDLTLNVSAGVDKLSDFGWLTNWSTGGNWAITKRFSLQASY
                     INNEAAPSLANLGAAAVTTYNVSTYDFTTGQTVLATVLTGGNAALKKESRHDIKLSMN
                     WTPPFLSKNSNFVVEYFDNHSSNVSASFPLLTAAVEDAYTDRVTRVNGTITQIDERPV
                     TIASEHQSRIRWGINLFGNMGKVVQSRSRFGMDGPPPGGPPPGEGHGGGDGGSKGGSG
                     GGHGAGGSGGPGGGGPGGPGGPGGPGGPGGGPSGPRHEGRWNLSLYHTVQLIDRVQLV
                     DGGAVLDLLHGDAIASTGGVARHSLEIDGGFFYKGFGLRTNTTWTAPTHVDGTGTSAD
                     LRYGALLKINARLFLDFNQQQRVIKAVPFLKNSRMSIMANNLLGSIQKVTNAAGEVPL
                     SYQAPYMDAYGRIIGFELRKMF"
     gene            21016..21261
                     /gene="infA"
                     /locus_tag="VFF98_02335"
     CDS             21016..21261
                     /gene="infA"
                     /locus_tag="VFF98_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041550738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZU62495.1"
                     /translation="MAKEELLEMRGTVVELLPNAMFRVRLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFMPGRGGPGSY"
     gene            21270..21860
                     /locus_tag="VFF98_02340"
     CDS             21270..21860
                     /locus_tag="VFF98_02340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HZU62496.1"
                     /translation="MTTPAAPLQLVLASSSPRRRELVGRLGVEPARIASPDIDETPAR
                     GERPRAYALRLAEEKARAVARAAGEVVLAGDTTVALGRRILPKAESADDVRAMLQLMQ
                     GRRHSCISAISVIDAEGRQRSRLNETVVAFKPLSAEEIEAYVASGEGLGKAGGYAIQG
                     RAEALVRFIAGSHSGVMGLPLFEARALLRAAGLPLG"
     gene            21860..22870
                     /locus_tag="VFF98_02345"
     CDS             21860..22870
                     /locus_tag="VFF98_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HZU62497.1"
                     /translation="MPVEWQVEEGLGETRALLIAHGEAVAARLAWPGALAAGAVIEAP
                     LFHRPKGARWGLARAANGEEVLVDRLPPDASEGATMRLAITRAAMAEQGRLKRALARP
                     TDAPLRPAPPLAETLAETLAGQGLPVRVVRRFPAGLWEEVFAQGWSGEVPFTGGGLLI
                     SPTPAMTLIDIDGTLPPPALALAAAGAVAEAIRRLDIAGSIGIDFPTLAEKAQRRAVD
                     EALAAALADWPHERTAMNGFGFVQIIARLEGPSLVARLARGRARAAALLLLRRAEQAE
                     GPGALLLVAPPALRAALALLPGDMEAQLARRTGRRLIWREDPSLALDAAYAQPLTEDL
                     LP"
     gene            22867..23064
                     /gene="yacG"
                     /locus_tag="VFF98_02350"
     CDS             22867..23064
                     /gene="yacG"
                     /locus_tag="VFF98_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZU62498.1"
                     /translation="MIAPARKCPICGQPRSAAHAPFCSTRCRDRDLLHWLQDGYALPG
                     PAAEDDSEGLPQPAPKDFSTE"
     gene            23150..23225
                     /locus_tag="VFF98_02355"
     tRNA            23150..23225
                     /locus_tag="VFF98_02355"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23183..23185,aa:Phe,seq:gaa)
     gene            complement(23434..23955)
                     /locus_tag="VFF98_02360"
     CDS             complement(23434..23955)
                     /locus_tag="VFF98_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZU62499.1"
                     /translation="MNAPMDSPLFREAMSRLGAAVNLITTDGPAGRAGLTATAVCSVT
                     DAPPTLLVCINRRSGSLATIMANGALAINVLRAHHAGMAARFGNRSLAVDEKFAGGDW
                     CFGESEPPFLGDALVSFLCHIASTVDVGSHCVMYCVVNRVHISDEDDGLFYFGRHYFG
                     LSREKVSLPEQGA"
     gene            24412..24762
                     /locus_tag="VFF98_02365"
     CDS             24412..24762
                     /locus_tag="VFF98_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62500.1"
                     /translation="MPHIVIEYAHTPSAQQAIDGLLDAVIARVRESPIIDIDSLKLRA
                     TPIHHYRVGGGHAPFIHTTVALMRGKDARSINELSTDLFRTIASHFPDIQQITVDVRE
                     MDPAPYRKRGKEHG"
     gene            24918..25550
                     /locus_tag="VFF98_02370"
     CDS             24918..25550
                     /locus_tag="VFF98_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62501.1"
                     /translation="MPNASSGNGQRARRTSTANPSNKKNKMLLRLAGALVDSPGASLE
                     WLAEMAETSRGTLYRIASSREELVVHLRNFVKETLNLVMDSAALETGEPLAVLSSLTR
                     GFLQYPTLCTFSAMSAHMNTPNGEPAVDIEDSYKSRMREFFGTWQKQGYFREDVPTEW
                     LVLFYDHVTQAASEGIRTGRLASAQAEPLVIESFLGGARARQDPAAATQD"
     gene            complement(25576..26406)
                     /locus_tag="VFF98_02375"
     CDS             complement(25576..26406)
                     /locus_tag="VFF98_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZU62502.1"
                     /translation="MTTPIFPELTEAVIAAMADAPDPRLRQIMTSLIRHVHAVVRETR
                     LTEAEWGQAIAFLTAAGQISNDRRQEFILLSDTLGVSMLVDAINHDDGPEVTPSTVFG
                     PFYSGLQPGLAMGASILMRPEAGTPTLVSGIIAGHDGMPIAGALIEVWQTAPNQLYDV
                     QDPDQPEGHLRGTFRTGVDGAYSFQTVLPVSYPIPDDGPVGKMLGALGRHPNRPAHIH
                     FMISAPGHRRLVTHIFIAGDAYLEGDAVFGVKPGLIVAPEKTAEGAVIHYDFRLAQGA
                     "
     gene            complement(26418..27485)
                     /locus_tag="VFF98_02380"
     CDS             complement(26418..27485)
                     /locus_tag="VFF98_02380"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HZU62503.1"
                     /translation="MRAFTYRGNPAHVIFGQGSLSRLGEAIAMVGGTRALLLSTPQQA
                     RQAAKACAGASAIVAHYDGATMHTPTDVTALALEVVQANGADCLVAFGGGSTIGLAKA
                     LALRTDLPQVAIPTTYAGSEMTPVLGQTENGVKTTLSSSKVLPELVLYDVDLTLGLPA
                     AMSGTSGINAIAHAVEGLYARDTNPIMQMMAGAGIKALAGALPKIAANPGDGEARSDA
                     LYGAWLCGAVLGAVGMALHHKLCHALGGSFNLPHAETHAIVLPHAWAYNAPAVPQAAA
                     ALKTALGTEDPARALFELGRAVGAPSGLKALGMPEAGIDKAADIAVANPYWNPRAIER
                     GAIRELIARAWSGLPPETAGA"
     gene            complement(27500..28744)
                     /locus_tag="VFF98_02385"
     CDS             complement(27500..28744)
                     /locus_tag="VFF98_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU62504.1"
                     /translation="MGETCADVVIVGAGHGGAQCAIALRQGGFAGSVAMIGREPEPPY
                     ERPPLSKEYFAREKTFERLYIRPPHFWAEKGVELHLGQEVVAVDPVARLLTLADGATF
                     GFGKLVWAAGGDPRRLSCPGAGLDGIHAVRTRADCDRLMGEIDGGVRDIVVIGGGYIG
                     LEAAAVLTKLGCRVTLLEALPRVLARVAGPELSDFYEREHRAHGVDLRTGAGVEALLG
                     EGRVSAVRLADGSEIPADAVIVGIGIVPAVAPLIAAGAQGGNGVAVDEYCRTSLPGVY
                     AIGDCAAFANPWAGISMRVESVQNANDQALAVARSILGDPQPYRAFPWFWSNQYDLRL
                     QTAGLSVGYDQTVLRGDPMNRSFSVIYLKGGRIIAIDAVNATRDYVQGRKLIEAGLSP
                     DPGRLGDTHTALKDLSCLKELS"
     gene            complement(28769..29317)
                     /locus_tag="VFF98_02390"
     CDS             complement(28769..29317)
                     /locus_tag="VFF98_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62505.1"
                     /translation="MPAEKTNEELWKVISELVAEAGARRVIARYMFLCDVPYPVYEGT
                     LDERIAAIGDCFTEDAVWEGVGGAHAASFGRMEGREAIRAHMRRFYAEVNATQNFNTH
                     YVCLGQVWATAEGAEGIWPQFQPWIYENGTSLLRSSRVRVRFREVGGEWKISHYRTEN
                     LFIADLPDNWTKSQIEKVLLTQ"
     gene            complement(29335..29835)
                     /locus_tag="VFF98_02395"
     CDS             complement(29335..29835)
                     /locus_tag="VFF98_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZU62506.1"
                     /translation="MSAVIEKAPVITLEEAARLIWREAELLDRHQYKPWLKLWTAGGL
                     YIIPADVAAVDYAEVLNIAYDDGPMREMRVKRLLSGFSMSSAPPARTVRTLSRFVEGE
                     AAGAQAIALRAAMIIVEYKYDRTRVLAADVDYRIVREAGELRIDRKVVTFINADDFLH
                     GIGYLL"
     gene            complement(29832..31109)
                     /locus_tag="VFF98_02400"
     CDS             complement(29832..31109)
                     /locus_tag="VFF98_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62507.1"
                     /translation="MGVVSENAGARDFGSLVLEDRVHTSLYTDPQIFEVEVDRIFKST
                     WVWVAHESEVPGPNTFRSSWVGTQPVIVTRDKADKIHVLLNRCRHRASSVCEKKQGKT
                     SVFVCPYHGWSYDMNGALRKVPYADGYAEDFDKSRFGLVSLRTESYNGMIFATFNDDI
                     MPLADYLGPARKWIDLFMKQGAGFGVTTLGEHKFRFPGNWKIQLENTTDSYHFPIVHK
                     TFLDSLDTETENLMDLIGSGGWVEDLGNGHSVAVMMPDLIDLEENLDAPIPERFADLA
                     AELAAEGRDDLDVRRIVRAVGGGGFNLNMFPNIACSMAFFRVLRPIAVNETEIRHVAI
                     GMKGGPAAANRMRMRLHEHFQGPMGFGTPDDAEGWERVQRGAQAGDDVYIMLNRGVAA
                     PEGGPEPGHNAGDASSEVGMRAAYQMWKRMMTA"
     gene            complement(31136..31762)
                     /locus_tag="VFF98_02405"
     CDS             complement(31136..31762)
                     /locus_tag="VFF98_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62508.1"
                     /translation="MALLRLENGEIFTDIARINALIGPMEIGIFSAPADFVAYANDPS
                     TRYDRAFANRLYTDVAPALEALLDEHGFGFQARRIGVYVPPAQPGDAHQFIALFNGDG
                     EAEPAPLRDEDFKAYLVPHRFPVLDFHFCFGGTMAKGLKLENGQEAVIYIFTGEWMRL
                     RPSVLNWPIFQSGSPIVGVSYFAEKENAHGAYDMELHPEYKPKDVASF"
     gene            complement(31875..33053)
                     /locus_tag="VFF98_02410"
     CDS             complement(31875..33053)
                     /locus_tag="VFF98_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62509.1"
                     /translation="MVQSNLFTQDEIKGIRDGFDGVRSSLPPRFYYDEDFYQWEVENI
                     LKKQWLIVGRQEEALNPGDYFTIRMFDEPIVIIRGRDGVLRALINVCQHRFAQIVEEG
                     RGNTKLMVCPFHRWTYNLDGTLQGVSVEQIPGFDKKNCRMPELRLEIWQGFVFINFDP
                     DAEPLAPQYAPIDPIFDAWKVADFQHSYTYEFEAPWNWKLTVEAGMEGYHHVGVHHDR
                     IEGEIPAKNTEEAWGGKACCSYHMWWKDGVPQEYLQPFGTPPGVEGVDWDNDPRVLFG
                     FPTMGTWLNNYQSTYLIFEFGNRVTANRGKICQAFPKWALETPGAEEAVKFQTDFAYQ
                     VQLEDEYACKMVQKGITSKTNGRAVLHPRLEVQMSKFYNWYLDKVLEAEGGAPLKLAV
                     "
     gene            complement(33189..34142)
                     /locus_tag="VFF98_02415"
     CDS             complement(33189..34142)
                     /locus_tag="VFF98_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62510.1"
                     /translation="MPQHKGLRTFDLNLLKTFIAIWDARSLTVAGEQLGLTQPAISHT
                     LKRLREQFDDPLFVRVGNEMVPTEVAVQLYAPLTASLQIVEQALLGQRSFRPAEANRT
                     FHLAMTDVSEYYFLSEILRIVRSQAPNVKIETINLDVTSIHALLKSGKTDIAIGYIPE
                     TEDYTMVPLIYDRHVCIVRKDHPFPGDELTVEDFAGLTFVDVSRRVPGYGVIEQHLSD
                     LHLERNVAARLSHLSVLPEMVRNTDLAALFPASVVSRFVSAGDFRVLGLPFDLPQIPI
                     SVHTHKRFAADPAIAWLRNAISIAFGGSNAAGGERCPSERN"
     gene            complement(34223..35320)
                     /locus_tag="VFF98_02420"
     CDS             complement(34223..35320)
                     /locus_tag="VFF98_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU62511.1"
                     /translation="MTIFVETLELGSAGGLRVAVKDSIDVAGCRTRDGSRAMANVAPA
                     TTNAEVVAHVLGAGCRIIGKTVMHELAFGLSGINDWAGTPVNPGYADLIPGGSSSGSA
                     AAVAAGLADFAIGSDTGGSIRVPAACCGIWGLKPTFGRVSRKGVAPAETSLDCVGPFA
                     RTIGMIEQAMAIIDPGFAPASVVAPRVGLVEVDADPAVTAACRGALVSAGLAMAPAHL
                     ALLEEAFAAGLVIINAETWAAFGGLVETGLLGADVEARLRAAAAVTHEQVAEAESVRA
                     AFTAAVDRLLYDHDALALPTLAGPPPTVAAVRSSGMDLAFSRFVRPFNLSGHPALSLP
                     VAGPAGWPVSLQLVGRKGEEAQLCAIARALA"
     gene            complement(35328..36824)
                     /locus_tag="VFF98_02425"
     CDS             complement(35328..36824)
                     /locus_tag="VFF98_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU62512.1"
                     /translation="MGYQGSFEIDAATRAFLERAPGIVVGGKRIAGRSAARIPVFDPA
                     SGLPIAEIVDAAPEDVNEAIERAHAAFQDGRWRHRTPAERERILFRFAQLIEDNGEAL
                     AQLETLEQGKSIHLSRLFASAGSAQWLRYAAGLTTKIEGRTMELSMPPGPDHWTAYTR
                     REPVGVVAGIAPWNFPMAIATWKFAPALAAGCSVVLKPSEVTPLSALWLAELALEAGV
                     PEDVFNVVTGTGQGAGAALVSHKLIRKITFTGSTATGKAIARAALENMVPTGLELGGK
                     NPAVVLADADLAVAVPGLMGGGFFNQGQVCSASSRIYVEEAIHDELVEALAGAIRSLP
                     VGAGLDPAAQVTPLVSREHKAKVTRYLDDARAKAQVIAGAPVPEGEGYFVSPHIVLDP
                     AETVALKREEVFGPVIAITRVASREEAVALANDTEMGLAASIWTQNLKAAMDLTRQLD
                     AGTVWVNTHSFIDPALPFGGTKQSGLGREFGTDWVNAYTELKSVCIAH"
     gene            complement(36844..38010)
                     /locus_tag="VFF98_02430"
     CDS             complement(36844..38010)
                     /locus_tag="VFF98_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62513.1"
                     /translation="MATKILETSTDGKINASAFNEVAFDHLLRVIEARRDEFDAKSHV
                     PRDVVQLMKAAGLYRAATPACFGGAGVPPHVFLERIERIARVDGSASWVAGFGSANTY
                     YAALPYETQRHIYASSPDQVFAGGLYPAVPAKRVDGGWRVSGRWRFASGCMGADWVAI
                     GLADEGTGLVRMAVSPAAEVKIIPNWDMVGMQGTGSYDTELTDHFIAEDWTCLRGTQP
                     IFDDPLYRYPLLAYQAQVHAASNLGLARAAIEVAAEMSGGKKLMPGAAKLADRAYFRT
                     GLARAEAMLQSCRLYYYDAAEQAWDVILRGDELSTPQRNALRLSATHAAHTAAEVIQL
                     LYRIAGMAAAARSNRLQQLLRDSLVVTQHASLSEATLELSGGALAGVELSPGFL"
     gene            complement(38153..39511)
                     /locus_tag="VFF98_02435"
     CDS             complement(38153..39511)
                     /locus_tag="VFF98_02435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU62514.1"
                     /translation="MAAFARKLWDILAGGEASLVPGVSRAHPVESDKALPSSVDVAVI
                     GGGIIGVSAALYLAKRGFSVAVFEKGAIAGEASGRNHGLIEGKHQDLRLQPLIELSKQ
                     CWRNLDTLTGEETGFREHDIVEFFNGEEELAGAQHWIDQAATIGLSARVASASQVDDL
                     LCGASSAYKYAFHAAGEGIAEPKLAAPAIAMGARKHGALIFQQCAVRGFERLGRGICA
                     IVTERGTVRTGKVILAGGVWTPTFLRSLGIELPMFGGYASIMSLAPFAGGPDLVGVLP
                     PVVVRRELDGGYAVGAIQGVAPILPSQFKYIRQLLPLLAAGPPIAPRLSWSNFWRDLT
                     TPATWRLDRPSPFEKVRILQPETQQPLLDRLLAELRRQRPEFMQAQVREAWSGALVST
                     IDDLPVLSGVSEVPGLFVGTGFTYGFTMGPGAGAVLAQLAAGEDPGIDLSPFQLSRFA
                     QA"
     gene            complement(39514..40515)
                     /locus_tag="VFF98_02440"
     CDS             complement(39514..40515)
                     /locus_tag="VFF98_02440"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU62515.1"
                     /translation="MNAINAVLVGVGFMGRLTARYMIENGIHIVGVVSRTSSQGEDIG
                     TLAGLPVPAGVAVTNDLAAILARGGVDIVVVTTSSDLESLSPIAELSLSHGVNVATIS
                     EEAFFPGTAGVIGQALNAVARANGATLAATGVQDIFWLNLPALLTGAVHRIGEVHCRS
                     NVNLDVYGPALIGQYPIDLTPEGYAKQAAEGEAKALIPFSGIALEAMVAKLGWTPTER
                     SVRHEPIYAEREIVSESLGRTILPGRTAGVTEIYQIETREGIKLEMEFVEKVNAPGET
                     EKITWTFRGEPELTFVTDNFPGVEVTCATLVNRLPDIIAAPAGYLSPSDLANSVIKG"
     gene            complement(40567..41838)
                     /locus_tag="VFF98_02445"
     CDS             complement(40567..41838)
                     /locus_tag="VFF98_02445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU62516.1"
                     /translation="MTKTQHVNSFYAASVASRLVDYPRLAGDIRADVCVLGGGFAGLS
                     TALHLAQKGTRVVLLERNRVGWGASGRNGGHMESLFGHDPDDFEKPIGADRASGMWEL
                     SSEACRLVGEIIARNAIECDMRKGVGHVAHKPKHAEHLRLGAEKFLARGYRKNVKYYD
                     RAELADLIGTEAYYGGFFDMDMVHLNPLQFAVGFAKVVAQAGVQIFEDSDALSYDASG
                     AGVKVRTATGTVMAEKLIIATNANIHRIDRHIAQKIFNAYAWVIATEPLGSARAAALM
                     KERLAVNDTRMFIDFYRLSGDNRLVFGSVSPISLDTEEKRKHVLRKRMLAIYPELADV
                     QIDYAWEGKGAADLKFLPHIGEASPKVYYTLASNVAWAVLNGQLIAEALHGDRSRFEL
                     VAGIETPAVPIGVRGREALVDAYKLYERTLG"
     gene            complement(41901..43082)
                     /locus_tag="VFF98_02450"
     CDS             complement(41901..43082)
                     /locus_tag="VFF98_02450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU62517.1"
                     /translation="MPSLTDGADWIQAVESRVRDGHAPGMVALVERSGQVRIHAAGCK
                     DLASGAPMQPDTLFRIASVSKPVTAVAALMLVDDGLLALDEDLGRWLPELADRRVLRT
                     AAAPLADTVPAKGPVTLRGLLSQTFGLGFDYAGWSSVSSRLQALHISAFNFAGDGDEY
                     MRRIGSLPLLSQPGERWLYHIGIDVAGILIERVAGERLGSFMARRIFEPLGMHDSGFR
                     LDPARERRLASLYAAQGGRYYQIADAPRRTQARPMDQGGSELISTAPDLAKFGRMLLD
                     GGTFQGRRLLAQGLVAEMRRDHVGPQAKQRSPQFPGFWDALGWGLGLCVVQAANHVSP
                     HPGRFGWWGGTGTSLFMDPATDSVLVMLSQRMINDISDSANADAFMRAALAELRSIHC
                     N"
     gene            complement(43087..44232)
                     /locus_tag="VFF98_02455"
     CDS             complement(43087..44232)
                     /locus_tag="VFF98_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62518.1"
                     /translation="MESPRTFGSSWAAYILCPLLLTIGACGLFVAPFVIEVASRIYRL
                     SEAAAGGIVTAEVIFSAFTALGLANVFSRIDVARLCFWALVITVAGNILSCLSYGVAW
                     FVVCRIVAGLSSGVLYASAAYWAAQHHAGVRVMSASFIVASVVYGLGFVVWPSAVLKY
                     GFVPIYGLLVALCLAAAAALAVFGCPRPSLTAEFEQHELEPKPAYALWVLLLMCALAN
                     GGLQMLWSFSLSVAAIRKFDVDTVGIVLGLSGVFNIIGSLLAGFLDRRFGVALPLALG
                     ILLGCLAGLSIGASGAMVWFATGIFLYGMSAFFVMPYLFSAGAALDNQGRAVTLSGFA
                     VYFAGALSPLLGGVIADRISLAAVGWVSAAACFGAAICALVLRSSLD"
     gene            complement(44267..44650)
                     /locus_tag="VFF98_02460"
     CDS             complement(44267..44650)
                     /locus_tag="VFF98_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62519.1"
                     /translation="MSDERGAIETVVFKYINGIRDSDPDLVASAFHAQAVMTGHFGGQ
                     FMVIPNAGAFVADYMRKAPPTHESSPDFEGRIVRVDQYGTLANVAIEENGLEGHDMRS
                     VFILHKIDGAWLIVQKGTWSPGGGS"
     gene            complement(44643..45233)
                     /locus_tag="VFF98_02465"
     CDS             complement(44643..45233)
                     /locus_tag="VFF98_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HZU62520.1"
                     /translation="MKNLYMFFVGGTAPGANIEIHDVQFAVVEKPEEAFAGIANRWFA
                     DQELLHIDVIRRVNWVDGYDVTIGSGPSQGEARLFFVNMGGYFREESFERHAFEVIAA
                     KSEVEAKAIAKSRFFNDHEIPHRDFLMDIDGFLALDKVGEEHIHLIANQYGAAELPSF
                     QGYLKIGGNKFVYDETIDDLVHEDEARLRVEGIGNE"
     gene            complement(45287..47518)
                     /locus_tag="VFF98_02470"
     CDS             complement(45287..47518)
                     /locus_tag="VFF98_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62521.1"
                     /translation="MKNITRSSLSDGVSVAAVLVATMLVSWSSSAIAETAVAETAATS
                     TAEAAPAVGEITVTAQRRSENLQKVPISVTAATAETLEKSNVSSVADLNMLAPSLTFA
                     EEGGWSQPHLRGIGTTATGPGIESAVAVYIDGVYQASMVGGAADFGNVKAVEVINGPQ
                     GTLFGRNATGGLIQIRTLDPSHTASGNATLGYGSYETLTASAYLTGEVTKNLAANVAV
                     DFSDQASGYGRNEATGKDIDKTRKIFIRSKLLWEPGSNLKFTLAGNYGYVRYDPVLAS
                     APGTVSLGGTGTNLRPHDLNGYYDPYGYIRSYGVSLTSEWDLEKVHIESITAFQRTES
                     LTVEDGALTPNPSYAENISLNEPHEQFSQELQFSSPSGGAVTWTAGLYYFHETAKYDP
                     VHLYGGLYYPVTDLIDYATIKTDSFAAYGQATWAITPETHLTGGIRFTTERRSAFLDS
                     QTLEVGSTVPDVTASGHKWFHAPTWRVSLDHSFSPQILAYASYNRGYKSGGFNPGITN
                     YNAVTGKYEVVPYEPEKLDAYEIGVKAQTRDKRFRLNVAGFLYNYSNIQVQSFQTGIL
                     DISNGARARFYGADIAADAVLTPRFSLHAGASLLHATFTYYPNADYTTSIPLSEGGGT
                     AYSTGDATGNRVPFAPKLTITASATYTIPSQVGDFQINTTYNYNSGWNAEPDGRLKQA
                     AYSLLGAQVGWTSKGGKFGITLWGKNLLNTDYAVALFSQYQGDVIQWAPPRTFGATGK
                     VKF"
     gene            complement(48088..48753)
                     /locus_tag="VFF98_02475"
     CDS             complement(48088..48753)
                     /locus_tag="VFF98_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZU62522.1"
                     /translation="MSNAPLNILHVSSSPQPNRSDQQFAGSHSRELTGYFLDRWRETR
                     PNDVIVERDVGVNPPAAITVDWLRGSFSDPAAHDDAIRAALAESDVLAQELLNADVIV
                     IGVAVYNFGVPAQLKAWIDNVVRVGLTFSVTGGEKGLLAPGKRLVIATSAGSRESGDV
                     ESGAGAAIAALREPLSHLGVAHIDAVSLYGALSKKDYFEESLAAARAQISALVADIQK
                     SFA"
     gene            49221..50033
                     /locus_tag="VFF98_02480"
     CDS             49221..50033
                     /locus_tag="VFF98_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate 5-dehydrogenase"
                     /protein_id="HZU62523.1"
                     /translation="MKPLIGRDTRLCMSLAARPGNFGSRFHNRLYELCGLDYVYKSFT
                     TTDLAGAIGGIRALDIRGCAISMPFKEDVIALLDALAPSASAIDSVNTIVNDAGVLTG
                     HNTDYGAVVDLVAGLDRALPFVLRGAGGMGKAVAAALSDSGFRDGTIVARNAAAGQAL
                     ADLYGYRWQAAPAGTAALLINVTPLGMVGADADALAFPEAMIAACEVAFDVVQYPPQT
                     PFLQAAAQMGKRCITGTEVATLQALQQFALYTGVTPSPAQVAEAAQWARAHP"
     gene            complement(50035..52059)
                     /locus_tag="VFF98_02485"
     CDS             complement(50035..52059)
                     /locus_tag="VFF98_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZU62524.1"
                     /translation="MPRIGRPEITFSLTTFAAAMLALGISFLIGLDRPYWAMLTVYIS
                     SQPLAAAALSKGVFRIGGTLIGAAASVLFVPLFVQEPILLSLVMALWVGCCLFASLLD
                     RTPRAYLFMLAGYTAALITFPAVDTPERIFDLAVLRGQEISIGVACSSLLHTLIPAHD
                     FEQAIAASIRGVRGGMNRWIADVLRRPEESAPPDPPGTMAAAMTELRLLATHLRFHPD
                     NGVLGRDAIMAMWDHLVLLLPLVDAIEDRIAQIHRLGGLSPALAELLRALGDWASQAP
                     ADLSASRADIEAALATWHAGLHPQGWEGLLELNLHARLAELVQRIEAAKWLVEHTLHP
                     ATPLPAALRPLLAQRTRRRWHRDGGLALRSAVAAGIGVFGSCLFWIYTAWPEGGVMAM
                     ICAVVCSFFATLDNPRPAQTSWLVWTALSLPIAALYLFAILPRLDTFEMLMAALAPTL
                     VGLGVFLGVPALYARMMTLVVGFCGAIALTDTLNADFAAFLNNNIAQLAGAAAAILTT
                     AMLRVIGVERAVGRILRRNWRDLARLASPRSSVTREEWNSLMVDRAALLAPRLAQLEA
                     VPGHALALSNGLREVRIGLNLIQIGTMSHELGSAGRAALAQVQLRAGALCAAQGRARP
                     ANLHQCRADALRALQDLVALCRLPTANAVERELLNILVGMTRALLPDSQL"
     gene            complement(52062..52532)
                     /locus_tag="VFF98_02490"
     CDS             complement(52062..52532)
                     /locus_tag="VFF98_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZU62525.1"
                     /translation="MSTMSERGAPGDARLYALTNAIQPARRAWQQVAAAALAQSGVSV
                     AVATPVLFVSRLGDGVLQARLAEEIGVHPAALARALASAEQGGLIERRAVPADRRARG
                     VCLTPRGRKLAAHMEARLTERRRAMLGDLPAEDIATTLRVLRLLEERVRAGKGR"
     gene            complement(52589..53302)
                     /locus_tag="VFF98_02495"
     CDS             complement(52589..53302)
                     /locus_tag="VFF98_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZU62526.1"
                     /translation="MRSLGKGIMAGMLLLALAGCQRQEAAMPESRASSAAQAAAPQGP
                     AVHIVALGDSLLAGYGLRTEEAYPPRLEAALRARGINAQVVNAGVSGDTTADGLARLD
                     FTLASQNPPPDLVLLSLGGNDMLRGLPVAQAKANLDAILTRLEDRHIRVVVLGMLAAP
                     NMGPDYAGPFNAMFAQLARKHHAALVPFFLKAVYQRPGLQLPDHIHPTAPGVEAMVAD
                     TIGVVTGALGCGQKAAACP"
     gene            53368..54066
                     /locus_tag="VFF98_02500"
     CDS             53368..54066
                     /locus_tag="VFF98_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU62527.1"
                     /translation="MTHPPSGKSAILARNLTLTLGSGGEATEILRGLDLEVPQGQTVA
                     LLGPSGSGKSSLMAVLTGLERASGGSLAVAGADFAALDEDGLARARRGRIGVVLQAFH
                     LLPTMTALENVMTALELAGTPDAEARAAVELQAVGLGHRLHHYPAQLSGGEQQRVAIA
                     RALAPRPALLFADEPTGNLDAATGAAIIELILARRAQTGATLLIITHDEALAARAQRI
                     IRIADGRIAADELA"
BASE COUNT         9663 a        18227 c        16977 g         9271 t
ORIGIN      
        1 ccgggcggcc gggcggccta tccctcctcg ctgctgatga acgccattcc cgccaaggtg
       61 gcaggcgttg gccggctggt ggtcaccacg cccacgcccc ggggtcaggt gaacccgctg
      121 gtgctggccg ccgcccatat cgccggggtg gacgagatct ggcgcgtggg cggcgcgcag
      181 gcagtgggcg cgctggctta tggcaccgcg cgcatccgcc cggtggatgt gatcaccggc
      241 ccgggcaatg cctttgtggc cgaggccaag cgccagcttt atggcgtggt gggcatcgac
      301 atggtggccg gccccagcga gatcctggtg attgccgatg gccggaacga tccgcgctgg
      361 atcgcggtgg atctgctcag ccaggccgag catgatccgg ccgcgcaatc gatcctcatc
      421 accgatgatg ccggttttgc cgatgccgtg gccgatgccg tggaggccga gctgaagctg
      481 ctggccacag aagcggccgc ccgcgccagc tgggaaacgc atggggtgat catcaccgtg
      541 gccgatcttg ccgccgaagc ccccgcgctt gccaatgccc ttgccgccga gcatgtggaa
      601 gtggcgaccg acacgcccca ggccctgttc gacgaaatcc gccatgccgg ttccgtcttc
      661 cttggccggc acacgccaga ggcggtgggc gattatgtcg ccgggcccaa ccatgtgctg
      721 cccaccgggc gccgcgcgcg cttttccagc gggctgtcgg tgctcgattt tctgaagcgc
      781 accagcttca tccagctaga cgatgccgcc cttgccgcca tcggccccgc cgctgtggca
      841 ctggccgatg ccgaggggct gcccgcccat ggccgctcca tctcggtgcg gctcggacaa
      901 taaggaaccc atgcgatgaa cccgcgcgcc cgcgccgccg cccgccttgc cgccgtgcag
      961 gccctctatc agcacgatat ggagggcacc ccgctcgcca gcctgctgga cgaattccac
     1021 cgccaccgcc tcggcgccga gatcgaggat gtgcagtata acgaggccga aaccgccttc
     1081 ttcgacgatg tggtgaaagg cttcctcgcc cgccgcgagg agatcgacgg caagctggcc
     1141 gaaaagctgg ccaccggctg gaagctggag cggctggaca agaccatgct gcaggtgctg
     1201 cgcgccggcg cctacgagct gatcgcccgc gccgatgtgc ccactgcctc ggtcatcagc
     1261 gaatatgtcg atgtggccca tgccttcttc gacgagcgcg aggccaaatt cgtgaacggc
     1321 gtgctcgacg cgctggccaa ggcggtgcgc tgaggcggcc ctgaccagcg aagccgcctt
     1381 tatcgaggcc ctgcgcggcc ttgtcagcac cccttccgcg cgcgccctgc tggatgattg
     1441 cgcggtgctg ccgctgggca cggaaacgct ggtgctgacg catgacatga tcgccgccgg
     1501 cgtgcatttc ctgcccgatg ccgatccggc cgacattgcc tggcggctgg tggccaccaa
     1561 catgtcggat ctggcggcca agggggcaga gccggtgggc gtgctgctgg gccacatgct
     1621 ggacgaggac gacacccgct tcctggccgg cctgggcgag gcgctggccc attaccatgc
     1681 cccgctgctg ggcggcgaca cgatcgccgt gcccggcggc accagggtgc tcgggctcac
     1741 cgccattggc cgcgccactt gcccgcgcgt gccctcgcgc acgggggcaa gggcgggcga
     1801 cgggctttac atcaccggcc ctgtgggcgc cgccatgctg gggctggagg cgatccgcgc
     1861 cggcaccccc gatccggccg ccagcctggc ctatcgccgg ccagaggcgc tgatggccga
     1921 gggccgcgcg ctggcgccgc tcgtcaccgc gatgatggat gtgtcggacg ggctgctgct
     1981 cgatgccgcg cgcatggccc gggccagcgg catcaccctg gcgatcgaca gcgccgccgt
     2041 gcccatcgcc acgcccgaaa accgccgcgc cgaagcgctg cgctggggcg atgattacca
     2101 gctgctgttc accgcgcccg caggggtggt cctgccggtg cccgcctttg ccatcggccg
     2161 cgcgctggat gccggccctg ccccgctgct ggtcgatggc gcgccagccg gcgaggaagc
     2221 cccgctgggc tatcagcacc ggggctgaac cgggacaaac tgggacaaag cgtccacccc
     2281 cggcgaacac ccttgcgccc gccggccccg ccccctagca tcacgcccgc atgggtattg
     2341 ttcgccaata agaacagacc cgggatgcaa cggcgaccat gccctgcggg gggccatgtt
     2401 ttcgcattcc ccgcaaggcc ggcgacaaaa ggggattgcc tgtgactctt gtcattttag
     2461 caatagggct gggattactg gccatagtct atggttttat taccagccgg caagtgctgg
     2521 gcgcgccatc gggcaatgcc cgcatgcagg aaattgccgc cgccatccag gaaggcgcgc
     2581 aggcctacct caagcggcaa tataccacca tcgccattgc gggcatcgtg gtggcagcga
     2641 tcgtcggcgc ggcgctgggg ctgatcagcg ccacgggctt tgtgctgggg gcgctgctgt
     2701 cgggcgcggc ggggtttatc ggcatgaatg tgtcggtgcg cgccaacgtg cgcacggcgg
     2761 ccgcggcgca gaatgggctg caggcgggcc tcaccctggc ctttcgcgcc ggcgccatca
     2821 ccggcctgct ggtggccggc ctcgccctgc tcgccatctc gggctatttc tattatctgg
     2881 tggcggtggc cggcgccgcg ccgggcagcc gggtggtcat caccgcgctc acggcgctgg
     2941 cgctgggcgc ctcgctggtg tcgatcttcg cgcggctggg cggcggcatc ttcaccaagg
     3001 cggccgatgt cggggccgat ctcgtcggca aggtggaagc cggcatcccc gaggatgatc
     3061 cgcgcaaccc ggccgtcatc gccgacaatg tgggcgacaa tgtgggcgat tgcgccggca
     3121 tggccgccga cctgttcgag acctatgtgg taacggtggg cgccaccatg gtgttgaccg
     3181 ccctgctgct gaagggcgcc gatgccctgc tgctgccgct gatgagcctg ccgctgctga
     3241 tcggcggcgt gtgcatcgtc acctcgatca tcggcaccta tttcgtgcgg ctgggccgcg
     3301 gccagaacat catgggggcg atgtacaagg gcttcctcgt caccgccggc ctctcggtag
     3361 tggccatctg gctggccatt tcggcagcgc tgggcggcga tatggaaagc cccatcggcg
     3421 ccaatgcctt caacgggcgg atgctgttct attgcgcgct ggtggggctg gtgatcactg
     3481 gccttatcgt gtggatcacc gaatattaca ccggcaccgg ctttcgcccg gtgcgctcca
     3541 tcgcccgggc ctcggtcacg gggcatggca ccaatgtgat ccaggggctg gcgatcagcc
     3601 tggaggccac ggccctgccc acgctggtga tcgttgccgg catcatcatc gcctacaagc
     3661 tggctggcct tatcggcatt gcctatgggg caaccgcgat gctggcgctg gccggcatgg
     3721 tggtggcgct ggatgcctat ggcccggtga ccgacaacgc cggcggcatt gccgaaatgg
     3781 ccgggcttga cgatgccgtg cgcgagaaga ccgatgcgct ggatgccgtg ggcaacacca
     3841 ccaaggcggt gacgaagggc tatgccatcg gctcggcggg gctggccgcg ctggtgctgt
     3901 ttgccgccta caccacggac ctgcgcgaat tcttccccgg tctgtcggtg aatttcagcc
     3961 ttgagaaccc ctatgtcatc gttggcctgc tgctgggggc gttgctgccc tatctgttcg
     4021 gcgcgctggg gatgacggcg gtgggccggg cggccggcga tgtggtggtg gatgtgcgcg
     4081 accagtttgc cgccaacccc ggcatcatgg ccggcaccag ccgccccgat tatgcccgca
     4141 cggtggatct ggtgacgaaa gcggcgatca aggaaatggt ggcgcccagc atgctgccgg
     4201 tcttcgcgcc gatcctcgtc tatttcctca tcagccaggt ggccgggcgc gacaatggct
     4261 ttgccgcgct gggggcgctg ctgctggggg tgatcgtggg cgggctgttc gtggcgctgt
     4321 cgatgaccag cggcggcggc gcctgggaca atgccaagaa atatatcgag gatggccatt
     4381 atggcggcaa gggatcagac gcccacaagg cagccgtgac cggcgacacc gtgggcgacc
     4441 cctacaagga caccgctggc ccggccgtca acccgatgat caagatcacc aatatcgtgg
     4501 cgctgctgct gctggcggcg ctggcggggg ggtgaaaggc ccccgccctc acccgccggg
     4561 gtgatagaaa tcatacactg tgcgcgccac cgccgctgac acgccggggg cgcgcatcag
     4621 atcgtcgagc gctgccgcgc gcacccggct ggcggtgccg aaatgcagca gcagcgcgcg
     4681 cttgcgggcc gggccaatgc cggggatctc gtccagcggc gaggcggtga tggcgcgcga
     4741 ccgcttctcg cggtgggcgc cgatgacgaa gcggtgcacc tcgtcgcgca ggcgttgcag
     4801 caggaacagc accggcgcat tcactggcag catcttctcg cgcccgtcgg ggaagtggaa
     4861 cacctcgcgc ccctcgcggc catggtgcgg ccccttggcg atggcgatga ggttgacatc
     4921 ctccaccccc agttcctcca gcgcggcctt caccgcgctc atctgcccct tgccgccatc
     4981 gatcagcacc agatcgggcc acatgccacg atcgcggtcc gggtcctcct ccatcgcccg
     5041 gccgaagcgg cgggtcatca tctcggccat catggcgaaa tcatcgttgc tgtcggcctc
     5101 tttcacattc cacttgcgat actggccctt ctggaagcct tcggggccgg caacgatcat
     5161 cgcgcccagc gcatgggcgc cctggatgtg gctgttatca tacacttcca cgcgctgcgg
     5221 cgcttcgggc agttcgagga attcggccat ctcgcgccag atcttcgcct tgctgccgct
     5281 ttcggccatg cgccgctcca gcgcctcggt ggcgttgcgg gcggcctgtt ccaccagccg
     5341 cttgcgatcg ccgcgctggg gcgtggcgat ctccaccttg ccgccggccc cctcgcgcag
     5401 ggcctcggcc agcagttccg cctcgggcag cgcgcgatcg agcaggatga cgcgcggcgg
     5461 cggcacttcc tcgtagaatt gcgccaggaa gctttgcagc acctcgccct cctccagccc
     5521 gtcggtatgg gcggggaaga aggcgcggtg gccccagttc tgcccgccgc gaatgaagaa
     5581 ggcctggatg gcgatctgcc cggccttggc ggccagcgcg aagatatcgg cattgggcag
     5641 gccctcggcg ttgatcgcct ggctgccctg gatgaaggtg gccgcgcgca gccggtcgcg
     5701 cagcaaggcg gcgcgctcga aatccagcgc ctcggcggcg gcagccatct gcgcttccag
     5761 ctcgcgctgc acggccgagg atttgccgcc gaggaaatcc ttcgcctggc gcaccagctc
     5821 ggcataatcg gccgcgctga tccgctcgac gcaaggcgcc gcgcagcgct tgatctgata
     5881 gagcaggcac ggccggtcgc gccggctcat gaaactgtcg gtgcacgagc gcagcaggaa
     5941 cagcttctgc agcgcgttga tcgtggtgtt gaccgagccc gcgctggcaa aggggccata
     6001 ataatggccg gccgaacgcc gcgcgccgcg gtgcttctgg atgcgcggga aggcatgatc
     6061 ggtgcgcagc aggatgaagg ggaagctttt gtcatcgcgc agcagcacat tgtagggcgg
     6121 gcggaagcgc ttgatcagct gcgcttccag cagcagcgcc tcggcctcgg aattggtcgt
     6181 gacgatggtc atcgcccggc actggctgac catgcgctgc aggcgcagcg gcagccggtc
     6241 tacctgggtg taattggcca cccggttgcg cagcgcccgc gccttgccca catacagcac
     6301 atcgccccgc gcatcctgca tgcgataaac acccggccgc gccggcagcg tggccagcac
     6361 ttcgcggatg gcggcaacgc cggcgtccag atcgggcttt tcgcgcccgc gcaccaggct
     6421 ggtggcggca tcctcgttga aacgctcggc gctgtcaggg tcgcgcgggg ggtctgtggg
     6481 cttcgacatg cgccccggat agacgcaagc cgcgcctttg gggagaggcc caaaggcgcc
     6541 ggcgccggca gacaggcccc ggcgccggaa aaagccccgg gctcagcgat cgtccagcgt
     6601 gtggaggaag gcgatgatgt cgtcgatatc ggcatcgctc agcggcggcg tgtcgccgcg
     6661 cttctgggtg aagggggcat cggctacatc gatgttgcca tgatatttgg ccggcagatc
     6721 attgtagatc agcaggcgcc cctgcgcatc gcgcggatag accttctccg gatgggcggc
     6781 gcgctggttg tagaaattca gcacgtcgcg cagattatga taaacgccat tgtggaaata
     6841 gaccccgcgc ctggcgctgt tgcgcaaggt gggggtgagg aacatgccgc aatattgcgt
     6901 ctgccgggcc agatcctggc gcgccgggcc gcacaggccc atatcgaaga aggccggatc
     6961 gcggttgacc ttgaggcgcg gattgcgcgg cacgcccagc gcctcgaatt cgctgtcggt
     7021 gaacagcggc ggcaggccat cggcggcggg ctggctgagg tggcaggcgg cgcaattgcc
     7081 cttctccggg tcgttgaaca ggcgcaggcc gcgcatttcg gcatgggtca gccgttcctt
     7141 gccctccagc caggcatcat acttgctgtt gaagcggtgg aacgcccccg attcgacctg
     7201 ataggccgac agcgccgaca tcgcctcgat gaatagcatc tcgggccgat ccagcgtgtg
     7261 ctggccaaac agccggccca gctgggcaag atagggcagg cggcgcagct tatccaccac
     7321 ggcgaaacgg tccttgttgg ccatttcggc ggggttcatc atcggcccca tcgcctggtc
     7381 catcagcatg tcgatccgcc cgtcccagaa caggccgccg gcgggcacca gggcggaagc
     7441 ctcggcaggg gtgccggcgc ttttcacttg cctggcctgc gtctgcgcct tgtcggccag
     7501 ctggtcgagc gggatcggca catcgagatc ggccgtggag gggccgatgg tgaagccctt
     7561 ctggcgatag agataggtga gccccggcgg cgggcgcagc ccttcctgat ccatggcaat
     7621 gccgcccagc tccacatcgc gggcagtggg cggaccaaag gcgcgggccg gatcatggca
     7681 gctggcgcag gattgcttgc ccgaggccga cagcgacgga tcgaaaaaca gtttctgccc
     7741 cacctgggcc agcacgctca gcggcttttc ggccgggcgc agcagcttta cggggtgggg
     7801 gttggcgccg gtcgtatcct cgatccagcg gcccatcgcg gcgggcgcct gggcgggaaa
     7861 ggcgaggaac cagcccagcg tggcgccgcc cgccaccgcc agcgcggcag cccccagcgc
     7921 caccttgcgc cagacgcgcc tccctcccga tgcgcggggg gcgttgtggc tggcggcagg
     7981 ttgggtgtca ggctcggtcg cggtcatggc agggctttgc aaaaaagaga tctcccgccg
     8041 gcgcgctggc cggcgggaga agagtagggt ggaaaccggc aggcctcagc tcgtggtgcg
     8101 cacgccggtg gtggtgttca gcagataggt gccggtgcgc ggcgaggagg tgaagtcgaa
     8161 caggctgcgg atatcgccgg cggcggcatc gttcgaacca ttgcccaggc gcttgccgcc
     8221 cagccagttg tcctcgatga agcggacgat cgacgactgc gagatctgcg tgctgtcgac
     8281 ataattctgc cgcgcatagg gcgagatcac caggaagggc gtgcgcgtgc ccgggccgca
     8341 gcggccgttc acggtcttgc ccgaaaggcc gacaccggcg gtggcgctgc tgctggtgca
     8401 gctgccggcg gcggtgaagg catcgtaggt ggcatccgag gaggaagtgg tgatcgtcgg
     8461 cgactggtgg tcgtaccagc cgtcggaatc gtcataggcg atgatgatcg ccgtgttctt
     8521 ccagtccgac tgctgcatga tgaagttgat ggcggtggtg atgaaggtct gctcgtccag
     8581 cgggttcgaa ttgcccgggt ggccatcctg gaaggcgggc gccttgatga agctgaccga
     8641 ggggaagttg cccgcgctga ccgccgtcac gaagtcgttg tagtcatact ggtggttgat
     8701 cgcggtggcg ccatacgagc tgtcggtggt ctcggtatag ccgatcatcg cggtcgagct
     8761 ggggcgggtg tggttgatgt tggcggtggt gccgtaatac tggaaccagg cgtggtgggg
     8821 gatgtagtcc gtcaccgtcg agctggtgtt gggcgacacg gtcgagcgcg cgcagccggt
     8881 ggtgttgttg ctgttggtgg tggccaggtt gaagccgccc atgaagccgc cccaggtgat
     8941 gccggccgca ttcagcaggt cgccgatgtt ctggccgctc atcttgacgg tggcgctggt
     9001 cgtgctcgag cacttgtcgc cggtgggatc agggtcgccg atgaccgaga aactgccgtt
     9061 gccatcggca atggcgctgc tggcaatggc cgagccggtg gaagtgaccg cgccattgtt
     9121 ctggcccgag atcacgttga tggcgcccgg cgtcgaggga ccgaaagtgt cggtccaggc
     9181 gttgtcgctc atcgcgtagt tctgggcata gttccacagg gcggtaacgg tgttgccgtc
     9241 gaaatagccg agcacctgcg ccttggtgcc gaactgggcc gaaccgctgg tggccgcctt
     9301 gccggtgtaa tagacaaagg catccatcag gccgttgtcg ctggcttcct gctcgggcag
     9361 gtaggcatgg ttcatgctcg tcacgttata ttgcgcgcga tcgatgcgga acgggttcag
     9421 atagctcgcc tgatagccaa ggctggtgtt caggctgttc gtcatgttgg ggttggcgtt
     9481 cagcagcgag gggtgcttga ggtagttgtt gacataggca acgttggagg gcgcggtcca
     9541 gctctgggtc ggctcgccgg tggggtcggc cgactgcgga taggtgccga aatagtggtc
     9601 gaacgactgg ttctcgggga agatcaccac cagatgcttg atcggcgtgg tggtggtcag
     9661 cttgtcctgc gcggtggtgt tgctgtcggt gctggtgttc ttgttatcgt cgtggcagcc
     9721 ggccagcagc agggcggcgc ctgcggttgc ggtcaaggcg agtgtcgtca aaagccctgt
     9781 tttgcgtttc atgtgaatag actccagatg cgctagcgcg gcgcgccggt gatctgaaag
     9841 ccccgccggt cagccgctgg aaaatcgggc cacgcccgcg cgccgatcca gcagcccaag
     9901 atgacagggc aatgacgatt tcccaagttt tccattgtct taatagtgac ccattcggcc
     9961 attacaaatc ttgcaacgca aatggccgcg caggtgaaag accttaatcc ggcatgcggc
    10021 ggccgacata aaaccgtcac caaaccgtcg catagcccgc tcgcccaggt tcttcgcaac
    10081 ctgctcaccg cttttgcccc acaccctttc gccaaggaca tctcgtgcgc acaggatcga
    10141 cagcgttggg aggcatgctg gccttgtgct gcagcatcat gcccatcgct gccccccgcg
    10201 cgcatggcca ggtgatcgag atcggcgatg atggcgtggt gcgccagcgc agcaacaccg
    10261 ccgccgtggg ctggaccacc ctgcccgccc ccggcgcacg ccccgccccc cgcgcgggcc
    10321 atggcgccag cgccctgccc gctgccagtg ttcttcccgc tgccacctcc ctacccgccg
    10381 gctccgtcga ggctgccggc gtgccggaga tctggcgcgc gcccttcgcg gcggcggcca
    10441 gccgctacaa tgtcagcccg catctgcttt ccgcgctgat ctggcaggaa agccgctggc
    10501 acccgcaggc gatcagcccc aagggcgcgg tgggcctggg ccagctgatg cccgccaccg
    10561 cccgcgccct ggctgtcgac ccgcgtgatc ctgaggccaa tatcgatggc gcggcgcgct
    10621 atctgcggct gatgctcgac acgttcgacg gcgatgtcgt gcgcgccctt gccgcctaca
    10681 atgccggcgc ggcccgtgtg gcgcgcgctg gcggcgtgcc cgccattgcc gaaacccgtt
    10741 cgtatgtcgc caatatcctc aaccgggtaa cggccaccac caccacagga ttgctcaaat
    10801 gatcaagcgc tttgcccttc gcgcccggct ggcggccctg tctgccgcca gcagccttct
    10861 ggcctcttcg ccggcccttg cccaggcggc cggcggcacc gatccggccg gttccggccc
    10921 catcgttgcc gccatgggct ggctgcaggg cacgctgctg ggcaatgtcg ccacggccgt
    10981 ggcggtgatc gcggtggcga tggtgggctt catgatgctg accgggcgga tgaactggcg
    11041 ctttggcgcc acggtgatca tcggctgctt catcctgttc ggggccagtg ccattgtctc
    11101 gggcattcag gccaccgccc aggccaccca ttgagccggg ctgacagctg atgaaccgcg
    11161 ctccggtttt ccgcgccctc acccggccac agatgttcgg cggggtcacc tacagcttct
    11221 tcgtcatcaa catggcggtg acgaccgagg ccttcctcat ctcgcacagc ttctgggcgc
    11281 tgccgctggc gctggtggtg catggggtgg gctatgtggc gtgcctgcgc gaaccgcgcg
    11341 tgttcgatct gtggctgacg cgggtcagcc gttgcccgcg ggtgcgcaac tgggcccgct
    11401 ggggctgcaa cagctacgcg ccatgagcgg gggcaaaacc gccagccggg ccaaggcgcc
    11461 ggcctggaag ggcccggccg catgggggcc cagggaagcg cgcgcagggg atcggctgcc
    11521 ctatgcggag ctggccgatg gcaccacgct gcgcctgcgc gatggctcgc tgatggcgat
    11581 gatccaggtg ccgggcctgc cgttcgagac agaagacgac gaggcgctcg accatcatct
    11641 gggcgtgcgc gagacgatgc tgcgctctgc gctggatgcc cgcttcgtgg tctatcacca
    11701 tgtgctgcgg cggcgggtgg aggtctcgct cgacaccccc ggccatggcg cgccggcaga
    11761 tccctttgcc gccctgctgc atagccgctg gcaggatcgc ctctccaccc gccggctgtt
    11821 cgtcaacgag caggtgctga ccgtgctgcg ccgcccggcc cgcggcaaaa ccggctggcc
    11881 cgaaaagatc aggcgccgcc tggccggcac cagcgccggc cccgatccgg ccgaccggcg
    11941 cgcgctggat gccgccgtta ccgcgctggt ggcagcgctg gaaccctatg gcgcgcgcgt
    12001 gctgggcagc tatgccggcc cgcgcggcgc cctgttttcc gaaccgctgg aaatcctctc
    12061 ggcgctgtat aatggcgaga tgcgcccggt ggccgtgccg ccccagggca ccgatatcgg
    12121 ccatcacctg ccctatgcgc gggtttcctt cgggctggat gcgatcgagg tggcgcagcc
    12181 atcgggccgc agctttgccg cgatgctggc ggtgaaggat tatcccgaca gcacccgcgc
    12241 cgggctgatc gacaatatcc tgcgcctgcc gcacgagctg gtgctgaccg aaagctttgc
    12301 ccccgccgac cgccagatcg cccgcgagcg gatagatctg gccatccgcc gcctgcgctc
    12361 ggccgacgag gaagcgggcg cggaacggcg cgagatgctg gccgcgcgcg atgcgctggg
    12421 cgcagggcaa gtgggctttg gcgatcatca cctctcgctg atggtgcgcg ccggcgatct
    12481 gcccgcgctc gaccgggcag cggcggcagc gggcgccgcg ctggcggatc tgggcgctgt
    12541 ggccgtgcgc gaggatgtga acacagagcc cgccttctgg ggccagtttc ccggcaacga
    12601 aagctatgtg gtgcgccgcg cgctgatctc cacggccaat gccgccggct tcctctcgct
    12661 gcatggcatg cccttcggcc ggccagaggg caaccactgg ggcccggccg ccaccgtgtt
    12721 cgaaaccacc agcgccaccc cctatttctt caacttccac gagggcgatc tcggcaattt
    12781 caccgtcatc ggcccctcgg gttcgggcaa gacggtggtg ttgaacttcc ttgccgccca
    12841 ggcgcagaaa ttcgcccccc gcaccgtctt cttcgacaag gaccgcggcg ccgagatctt
    12901 cctgcgcgcc attggcggcc attatgcccg catccagccc ggcgagccga ccggcttcaa
    12961 cccgctggcc atgcccgaca ccgcgctgaa ccgcgccttc ctgcgcgact ggctgggggt
    13021 gatgctggcg gcggaagggc ccgaggaact ggccagcatt gcccaggcgg tggatgcctg
    13081 catggaggca gagcccgcct ttcgccgcct gcgccacttc cgcgaattgc tgggcggcgc
    13141 gcgccggccg gaaccgggcg atctgccctc ccggctggat ccctggatca acaccggcga
    13201 acacgcctgg ctgttcgaca acgcccatga cgcgctcgac cttgatgccc gcacgctggg
    13261 cttcgacatg accgcgctgc tcgacacgcc ggcgctgcgc acgcccgcca tgctctacct
    13321 cttccaccgc atcgacgagc ggctggatgg cacccccacc atggtgctga tcgacgaggg
    13381 ctggaaggcg ctggacgacc ctgtctttgc cgcgcgcatc cgcgactggc tgaaaaccct
    13441 gcgcaagcgc aacgcgctgg tgggctttgc cacccaaagc gcgcgcgatg cgctcgacag
    13501 ccgcatcgcc agcgccatcg tcgagcagac cgcgacgatg atcttcaccc ccaacccgcg
    13561 cgcgcggccc gaggattatt gcgaaggctt cggcctttcc gcgcatgagc tggaactggt
    13621 gcgccagttg cccgcccaca gccgctgctt cctcatccgc catgccaatc attcggtggt
    13681 gacgcggctg gatctgggcg gcgaggcgga tatcctcacc gtgctgtcgg gccgcgaggc
    13741 gagcgtgcgc cggctggatg gcctgcgcgc ccggctgggc gatgcacccg ccgcctggtt
    13801 cgaggcgctg accggcaccc cctggcccgg ccgcgcagac gtgcaggccc cccccctgcc
    13861 gagcgctgcc gaatgaacgc ctgccccgcc atgcagctgg atggcgccag ccttgcgcaa
    13921 accctgcgcg ggctggattg cgccatgggg gcgggcacgg ccacggcctt ttcccgcctg
    13981 tttggcagcc atggcgcgct gatgccggcg ttgaccgggc tgctcacgct ttatgtcggc
    14041 ttcttcggct gggggctgct taccgggcgc acccggctgg gcctgtctgc cctcaccccg
    14101 cgcatgctca ccatcggcct ggtgatgacc tttgccacct cgtggatggc ctatcagaac
    14161 gtggtgtggc aactggcggt gggcgggccc gatcagatcg ccaatgtgat cgccggcggc
    14221 cacggatcgg cgatgaacgc ctttgccgac aagatcgaga tcatcttttc cgccattgcc
    14281 gatgccacgc atcaaagcgc ggccggcgcc gtggcggcca gccaggccac cgccgatggc
    14341 gcctcgcgca cggtctcctt cctctcgccc cagggcatgc tgtggatttc cgggctgatg
    14401 ctgctggtgg gcacggtggg ggtgatggtt accgccaaga tcgtgctggc cgccttgctg
    14461 acgctgggcc cggtgttcat cgtgatggcg ctatttccgg ccacgcgcgg gttgttcgag
    14521 ggctggctga aggcctctgt cgccagcgcg ctggtgccct tgctgacggt gctggtgggc
    14581 ggggcgatgc tctcgatgat cgcgccgctg atccgcgatg cgctcaatga ttccaccgag
    14641 caggcgacgc gcggcgcgat gatgctgttc ctgtcggcct gcgtctatgt ggcgctgatg
    14701 gtgctgctgg ggcgcaccgc ggcgatgatc gtctcgggct ggcggctgcc tttgggccag
    14761 cccccggcgc cggccacagt ggtggcggca gcggcagcgg ccaccgccgc gcccgccatc
    14821 accgccggca tcgccccctc ttttgcgcag gccgccaacc cgcgtgtgcg cgccatgatg
    14881 gccggcctcc aggcctctgc ccgcgaggcg gccgacagcg ccggcaccag cgccgaacgc
    14941 accgaaaggg cgcgcacgct ggtcatgggg ctggcgcctg tggcagccag tgccgcggcc
    15001 agccccggcg atcgccgcct gcagggcgtg ggcagccgct ttcgcccccg ccccgatgcc
    15061 tccacccctt ccgccaaggc ccacccgtga agcttgatcc gctctcttcc tccgcgcttg
    15121 ccctgctggc agctgccttg cccgttctgg cgcccgctgc cagcgcccgc catgcacgcc
    15181 acgatgcgcg cctggtgccg gctgccggtg cccatgatgc acggatcgtg ggccgcctct
    15241 atgatccggg cgtgatcgtc accgtgcgcg gccataccgg catccagacg acgatcgagt
    15301 tccgcgccga cgagcggatc gacaatatcg ccgtgggcga ttccaccgaa tggcaggtga
    15361 cgcccaacaa gcgggcctcg ctggtcttcg tcaagccgat gaaggccagc agccacacca
    15421 acatgaccgt catcaccgac cggcgcacct atctgttcga tctggtggcg cggcccgggc
    15481 tggcgccggc ctatatcctg cgcttttcct atgcgcccga tccggtgccg ccgccgccgc
    15541 ccacgctgct gccggtggag gccgagattg cccgggccgg cagcccgccg cccgaaagca
    15601 gcccggccga cctccatttc aactgggtga tgagcggcat gcgcagcctg tggccggccc
    15661 gcctgttcga tgatggccag tccgtctggc tgagctggcc ccgcgatgcc gagccgcccg
    15721 ccatcctgcg ccacgatccg gccggcggcg acgggccgct gaactatcgc atcgcgggcg
    15781 attacatcgt ggtggacggg gtgcccgcgc aaatcaccct gaagcgcggc aagcagaccg
    15841 ccacgctgat ggcccccgcc cgcctggtgc ccggcagcga tgcccccgcc ggcaaaacgc
    15901 cgcgccatca caagccgcaa ggcaccgtgg ccgcccctcc gccagcagca gaggccggca
    15961 aggacagcgc tgcccccgcc gccgccagca caggccccca cccatgagcc ccgcccccac
    16021 accgccgaac cgcccgctgc ccaaccgccc gctacccagc cctgccggcg agggcgatcc
    16081 gcgcctgaag atggatgacg aggcactggc catggccagc cgcaacgctt tcccggtggt
    16141 ggcgcaggcc cggcgccgtt ccgacacgat ggggctggcg ctgggcgcgc tggtggccgt
    16201 gctgttgggc gcggtcacgc tggtctcact cagccgccac cgcaccgcgc ctgcggccga
    16261 ccgcctggcc accgccctgc ccacccccgc gcccagcgcc acgccgcacc ccggcttcac
    16321 gcccattgcc ctgccgctgc cggtcacgcc ggccaaggcg atggcgggca tttcggccgt
    16381 ggcgattgcc ggggccgcct cgccggcgat ggtgtacgac gggccgagcg ggcctgcggt
    16441 ggccgcccct gccgcagccc ctgccgccgc ccacggcggc gagggcgaag gcaaggacaa
    16501 gagcgaggac accgaccagt tcctcaccaa ggccaacagc gcggtggata ccgccaccgc
    16561 cgagaagatg ggcgatccgg ccaacaccat cacgcagggc acgctgatct cggccgtgct
    16621 ggaaaccgcg ctggattccg acctgcccgg ctatgtgcgc gcgctcgtca ccgagaatgt
    16681 gcgcagcttc gatggctcca gggtgctgat cccgcgctat tcgcggctga tcggccaata
    16741 caagtcgggc ctcgccaccg gccagcagcg cgcctatgtg gtgtggacaa ggctcctgcg
    16801 gcccgatggc gtgtcggtgg cgctgggctc gccgggcatt gattttggcg gccagtcggg
    16861 cctcaccggc aaggtctaca gccattacct gcagcgctat ggtccctctg tcgtgctctc
    16921 ggtgctaacg gggctcgcca cccagtcggg caattcggtg gtggtttcca ccggcaacac
    16981 cgcctcttcc gtggccgccc agcacgacac ccagatcccg ccgacgatca aggtggccgc
    17041 cggctcgccg atccgggtgt tcatggcgcg cgatctcgat ttttccagcg tgaccggcgc
    17101 gggccgcaag ccgtgaacct gcccgcgagc gacaccgcca gcccgccggg cggggtctat
    17161 ctcaacgcct atctggcgcc gctggaacaa tggctggcac gcgaggatgt gaccgagatc
    17221 ctcgtcaacc gcccgggcga ggtgtggatc gaggcggccg ggcaaggcat gctgcgccat
    17281 gccgcccccg ctatcgacga ccgccacctg cagcgcctgg ccgagcaggt tgcccgcgtc
    17341 agccaccagg gcatcaaccg cgagcggccg ctgctgggcg cggtgctgcc cgatggcgcg
    17401 cgcgtgcagt tcgtggcgcc ctctgccacg cgggcccatt gggccatggc catccgccgc
    17461 caccgcatgg tcgaactgcc gctttcggcc tatgatcacg gccccctgcc gccgccgccc
    17521 acgctggccg gcgtgcccga tcccgcggcc gatccggtgg gttttctcca ccatgcggtg
    17581 caggcgaagt ggacggtgct ggtttcgggc ggcacctcca gcggcaagac gaccttcctc
    17641 aacgcgctgc tgggcgcggt gccggccgaa gagcgcgtgg tggtgatcga ggatacgccc
    17701 gaaatccgcc tgcgccatgc caacagcctg ggcctggtgg cggtgaaggg cgagatgggc
    17761 gaggcgaagg tctctaccga cgatctgctg caggcctcgc tgcggctgcg gccagaccgg
    17821 atcgtggtgg gcgaattgcg cgggcgcgag acggtgagct tcctcagggc catcaacacc
    17881 ggccatccgg gctcgttctc caccatccat gccagttcgc ccgccggcgc gctggagcag
    17941 ctggctctga tgacgatgca ggccggcctt ggcctcagcc ggcacgacac gctggaatat
    18001 gcccgcacga tgatcgatgt gatcgtgcag ctggaccgcg cgcagggaca aaggggcatc
    18061 cgcgcgatcg aatggctgcg ccggcctgat tgatagctgc gccggccggg ctgatgcccc
    18121 gccggcgccg ccgggccctc agaacatctt gcgcagctcg aagccgatga tccggccata
    18181 ggcatccata tagggcgcct gatagctgag cggcacttcg cccgccgcat tcgtcacctt
    18241 ctggatcgag cccagcaggt tgttcgccat gatcgacatg cggctgttct tgaggaaggg
    18301 cactgccttg atcacccgct gctgctggtt gaaatccagg aacagccgcg cgttgatctt
    18361 gagcagcgcg ccatagcgca gatcggcgct ggtgccggtg ccatcgacat gggtcggcgc
    18421 ggtccaggtg gtgttggtgc gcaggccaaa gcccttgtag aagaagccgc cgtcgatctc
    18481 cagcgagtgg cgcgccacgc cgccggtgct ggcaatcgca tcgccatgca gcagatcgag
    18541 caccgccccg ccatcaacca gctgcacccg gtcgatcagc tgcaccgtgt gatagagcga
    18601 gaggttccag cgcccttcat gccgcggccc gctgggcccg ccgcccgggc caccaggacc
    18661 acccgggccg ccggggccac ccggcccacc gccccccggc ccgccggatc cgccggcacc
    18721 atgccccccg ccgctgcccc ccttgctgcc gccatcgccg ccgccatggc cctcgcccgg
    18781 cggcgggccg cccgggggcg ggccatccat gccgaagcgg ctgcggcttt gcaccacctt
    18841 gcccatgttg ccgaacaggt tgatgcccca gcgaatgcgc gactggtgct cgctggcgat
    18901 cgtcaccggg cgctcgtcga tctgggtgat ggtgccgttc acccgcgtca cgcggtcggt
    18961 ataggcgtct tccacggcgg ctgtcagcag cgggaaggaa gcggacacat tgctggaatg
    19021 attgtcgaaa tattcgacga cgaaattgga gttcttcgac aggaagggcg gcgtccagtt
    19081 catcgacagc ttgatgtcgt ggcggctttc cttcttcagc gccgcattgc cgccggtcag
    19141 cacggtggcc agcaccgtct gcccggtagt gaaatcatag gtcgagacgt tgtaggtggt
    19201 gaccgccgcc gcgcccagat tggccagcga gggcgccgcc tcattgttga tatagctggc
    19261 ctgcaggctg aagcgcttgg tgatcgccca gttgccgccg gtggaccagt tggtcagcca
    19321 gccgaaatcg gagagtttgt cgacccctgc cgagacattg agcgtgagat cgcccagcgc
    19381 ctcgccgaaa tgttcgcgcg tgctggtgag agggatcgcc aggttgaagc cggtggaggc
    19441 atcgccgcgg cggaaacggg tggaggtgtt gttggcggca ttggtgttgc cggtgctggt
    19501 ggcttcctgc caggtatagg caaagccggc cttcaccgtc agcgcgatat cgccggccgg
    19561 cccatgcacc gggcggccca tcagcgtcac caggctggtc agcgtgtcgg tgcgggtgct
    19621 ggccaccgca aggcccgcat agggcagcgc gggcagcgtg ccatagggat cggccagggt
    19681 gatgccgctg gtgtcggcgc ggtcgtcggt gcgggtcacg gcatcggtgt ggctgccatc
    19741 gaccgtggcc gaaagctgcc acttgccgcc aacattcttg ttgaagctgg tgccggcctg
    19801 gatctcgtcg gtgcgagtga aagtgcccag cgcgccgggg aaggtgcgca ccacatcggc
    19861 gccgccggcc cctgcgccgg tgatcgtcag cgtgttgagg ccgttcttcg catccgtctc
    19921 ggtgcgggtg agcgcgccgt tcatcgccag cgagccgccg gtggggccag agccgatggg
    19981 gatcgtgtag gtggcgttca gcttgtagtc gcgcgtgtcg gagaccagcg tgcggttatc
    20041 ggcctggctg gggtctgtcg ccacgctcga ctggctgctg gcggcctctg ccacatggcg
    20101 ggtggcctcg gtcatcggcg tggtgtgatc gaccgaggcg gcgatgttca gccggcggtt
    20161 cttgtcgatc ttggcatagc gggccgagcc cttgacggtg gaagtgccgc cggcatcggg
    20221 ctgggtatat tccgcctcca cagagctggc gcggtaatgc tctttcagga tgatgttcac
    20281 cacccgctga tcgggcgcat agccatagcg cagcgccact tcctcgggca gcacctcgat
    20341 cttgcggatg gcctctggcg gatagtcgcg caattcgcgg aaggaggcga tgcgctggcc
    20401 gttgacgagg atgatcggcc catccgacga gcggccgcgc ccggaattgg tctcggccga
    20461 aatcgcatcg atcagatccg agatcgagct gacgccatag gaagcgatat cctcctcgtc
    20521 gaaagtgacg atgggcgcct ggggggcatc cacctggccg gcgatcttgc ctgccaccac
    20581 cacgatcggc cgcttgccgc cgccttgcgc gctgctgtca gcggaagagg tggtgtcagc
    20641 ggaagaggtg gtgtcggcag aagaggcagt gtcggccgca gaagcgctgc tgccctgcgt
    20701 ttgcgccaag gcggccccgg gcagcagcag caccgacaga accgaagaaa ccgacagatg
    20761 atatggcctg cgcaaggcgc gacactccct tgaatattga tttgttcagg ccccgatccc
    20821 gccctgccgg atgggccatg cgcatgaccg cggcaagaca ggggcgcgta tcattttgtc
    20881 gcatcgctga tacggttttg ccggggcgat tcatcgccgg gtcattaagg ccaagccaag
    20941 gctttccttt tgcaccgctt gcgtctatgg gcagccccga acctccctga cagaccctgg
    21001 aaaccgacag accggatggc aaaagaagaa cttctcgaaa tgcgcggtac ggtagtggaa
    21061 ctgctgccca acgccatgtt ccgcgtgcgc ctcgaaaacg atcacgagat cctcggccac
    21121 accgccggca agatgcgcaa gaaccgcatc cgcgtgctgg tgggcgatga agtgctggtg
    21181 gaactcaccc cttacgacct gaccaagggc cgtatcacct atcgcttcat gcccggccgc
    21241 ggcggccccg gctcctactg agcgggccga tgaccacgcc agcggcgccc ctgcagctgg
    21301 ttctggcctc ttccagcccg cgccggcgcg agcttgtcgg ccggctgggt gttgagcccg
    21361 cccgcatcgc cagccccgat atcgacgaaa cccccgccag gggcgagcgc ccgcgcgcct
    21421 atgcgctgcg cctggccgag gaaaaggccc gggcggtggc gcgcgcggcc ggcgaagtgg
    21481 tgctggccgg cgataccacc gtggcactgg gccggcgcat cctgcccaag gcggaaagcg
    21541 ccgatgatgt gcgcgccatg ctgcagctga tgcagggccg ccggcacagc tgcatctcgg
    21601 cgatcagcgt gatcgatgcc gaagggcgcc agcgcagccg cctcaacgaa accgtcgtcg
    21661 ccttcaagcc gctctcggcc gaggagatcg aggcctatgt cgccagtggc gaagggctgg
    21721 gcaaggccgg cggctatgcc atccagggcc gcgccgaggc gctggtgcgc tttatcgccg
    21781 gcagccattc gggcgtgatg ggcctgcctc tgttcgaggc gcgcgcgctg ctgcgcgctg
    21841 ccggcctgcc gctgggctga tgccggtgga atggcaggtg gaggaagggc tgggcgagac
    21901 ccgcgccctg ctgatcgccc atggcgaggc ggtggccgca aggctggcct ggccgggcgc
    21961 tctggccgcc ggcgcggtga tcgaggcgcc gctgttccat cgccccaagg gcgcccgctg
    22021 gggcctggcc cgcgccgcca acggcgagga agtgctggtg gaccgcctgc cgcccgatgc
    22081 cagcgagggc gccaccatgc gccttgccat cacccgcgcc gccatggccg aacagggccg
    22141 gctgaaacgc gccctcgccc ggccgacaga cgcccccttg cgccccgccc ccccgctggc
    22201 cgaaacactg gccgaaacat tggccggcca gggcctgccg gtgcgcgtgg tgcggcgctt
    22261 tccggcgggg ctgtgggaag aggtctttgc acaagggtgg agcggcgagg tgcccttcac
    22321 cggcggcggc ctgctcatct cgcccacccc ggcgatgacg ctgatcgata tcgacggcac
    22381 cctgccgccg cctgccctgg cgctggcagc ggcaggcgct gtggccgaag cgatccgccg
    22441 gctggatatc gccggctcga tcggcatcga ctttccgacg ctggccgaaa aggcccagcg
    22501 ccgcgcggtg gacgaagcgc tggccgcagc cctggccgat tggccgcatg agcgcacggc
    22561 gatgaacggc tttggctttg tgcagatcat cgcccggctg gagggcccct cgctggtggc
    22621 gcggctggcg cgggggcggg cgcgggccgc tgccctgctg ctgctgcgcc gcgccgaaca
    22681 ggccgaaggg cccggcgccc tgctgctggt ggcgccgcct gccctgcgcg cggcgctggc
    22741 gctgctgccg ggcgatatgg aagcgcaact ggcccgccgc accggccgcc gcctgatctg
    22801 gcgcgaagac ccgtcgctgg cgctcgacgc cgcctatgcc cagcccctta ccgaggatct
    22861 gctgccatga tcgcccctgc ccgcaaatgc cccatctgcg gccagccccg cagcgccgcc
    22921 cacgccccct tctgttccac ccgctgccgc gaccgcgatc tgctgcactg gctgcaggat
    22981 ggctatgccc tgcccggccc tgccgccgag gatgattccg agggcctgcc ccagcctgcc
    23041 cccaaggatt tttccacaga gtgattttcc tgcttgccaa cagcgccgcg cttggccata
    23101 ggcgcccgac caaacgctga caggcggctc caaacgccct cagccagatg cccgggtagc
    23161 tcagttggta gagcacgtga ctgaaaatca cggtgtcggc ggttcgattc cgtccccggg
    23221 caccaccacc aatcatcaga cctctgcacg aaatgccttc gggcatgtgc cacgttccga
    23281 acggtgttcg cgcagacgat ctgaagtctc gtcaggccgg actgcctgat ggccgccatc
    23341 cacaatggca gcgcccggca gccacatgca tcgctgactg cgccctacac gccgcctggc
    23401 gcagcaggat caagaccggg gctaaaaccg tgatcacgcc ccctgttctg gcagggaaac
    23461 cttctcacga gacaatccga aatagtgtct tccaaaataa aacaatccgt catcctcgtc
    23521 ggatatatgc accctgttga ccacacaata catgacacag tggctaccaa catccacggt
    23581 gctggctata tggcatagaa agctgaccaa tgcgtcgccc agaaagggcg gctccgattc
    23641 gccgaaacac cagtcaccac ctgcgaattt ttcatcgacg gcaagcgagc ggtttccaaa
    23701 gcgggctgcc atgccggcat gatgcgcacg caacacattg atagccaatg ccccattggc
    23761 cattatcgtc gccaatgagc cggaacgcct gttgatgcaa accagcagcg tcggcggtgc
    23821 atcggtaaca ctgcacaccg ctgtcgccgt caatccggcc cggcctgccg ggccatccgt
    23881 ggtaatcaaa ttgaccgccg cccccagccg ggacattgcc tcgcgaaaga ggggggaatc
    23941 cattggcgcg ttcataaaaa tccgctccca atatatttta tatatctgag gtgtaattca
    24001 aaaaacactt tcaatgtatt atgcacccat cattcgggtg ccttgtgcca aataccgaaa
    24061 aattcgatat cgccagccat ttcgaagccg tataatccgg cacatgggca tataattcaa
    24121 atttatatta attattttaa aaattatccg aactaatgga gcgatgcatt ggcatgaagg
    24181 cacccattga ttatcgaaat aaccaaatca acacatcgga aatgaccgat tattgttcat
    24241 atatacatta cctgttgacg agcccccgca tccaaggtga atgcaggcgc gggttcatga
    24301 gagtcggcca ggccacacac cgcccgcacg ctggctttca agcggccccc cgcactccca
    24361 ccaacccgtt gcagatataa cgccgcaaaa acgaacagga cgaggcccgc aatgccccat
    24421 atcgtcatcg aatacgcgca tactccatcc gcccagcaag ccatcgatgg cttgctcgat
    24481 gcggttatcg cgcgcgtcag ggaatcgccc ataatcgaca tcgacagcct gaaactgcgc
    24541 gcaacgccca tccatcacta ccgtgtcggc ggcgggcatg ccccgttcat tcacacaacg
    24601 gttgccctga tgcgtggcaa ggatgcgcga tcgatcaacg agctgtccac ggacctgttc
    24661 cgcaccatcg catcgcactt tccagacatt cagcagatta ccgtcgatgt gcgcgaaatg
    24721 gatcccgcgc cttaccgcaa gcggggaaaa gagcatggct gagctgatga tcggcgcccc
    24781 cgtcgatcac ggcgctggcc gaggcgcgca tcgttgacaa acctcgcccg gtgatagaat
    24841 gaggcaattg agtttcattt gaaccaatat gccccaaaca gaacaaacaa tgttccacag
    24901 gagtggattg cagcaaaatg cccaatgcaa gtagcgggaa cggccagcgc gcccggcgca
    24961 catcaaccgc caacccctcg aataaaaaga acaagatgct cctgcgattg gccggcgcgc
    25021 tggtcgattc tccgggggcg agcctggaat ggctggcgga aatggccgaa accagccgag
    25081 gcacgcttta ccggatcgcc agctcacgag aagaactggt tgtgcacctg cgcaacttcg
    25141 tgaaggaaac gctcaacctg gtgatggaca gtgccgctct ggagaccgga gaaccgctgg
    25201 ctgtcctgtc gtcgttgacg cgcggttttc tgcaatatcc aacgctgtgc acgttctccg
    25261 caatgtctgc acatatgaac acgcccaacg gggaacccgc cgtcgacatc gaggactcat
    25321 acaagtcccg catgcgggaa ttcttcggca catggcagaa gcagggctat tttcgcgagg
    25381 atgtcccgac ggaatggctg gttctctttt acgatcacgt cacccaggcc gcctcggaag
    25441 gcatccggac aggcaggctg gccagcgccc aggccgaacc gctcgtgatc gaatccttcc
    25501 tgggcggggc cagggccaga caggatccgg cggccgccac ccaggattaa cccgcggcaa
    25561 ggccccaacc gcccgtcagg cgccttgtgc caggcggaaa tcatagtgga tcacggcccc
    25621 ttcggcggtc ttttccgggg ccacgatcaa gccgggcttc acgccgaaca ccgcatcgcc
    25681 ctcgagatag gcatccccgg cgatgaagat atgcgtcacc agccggcgat gccccggcgc
    25741 cgagatcatg aaatggatat gcgccgggcg gttggggtgg cgccccaagg cccccagcat
    25801 cttgcccacc gggccgtcat ccgggatcgg ataggaaacc ggcagcacgg tctggaagct
    25861 gtaggcccca tccacgccgg tgcggaaggt gccgcgcaga tggccctcgg gctgatcggg
    25921 gtcctgcaca tcgtacagct ggttgggggc cgtctgccat acttcgatca gggcgccggc
    25981 gatgggcatg ccatcatggc cggcgatgat gcctgaaacc agcgtcggcg tgcccgcttc
    26041 cgggcgcatg aggatcgaag cgcccatcgc cagccccggc tgcaggcccg aatagaacgg
    26101 cccgaacacc gtcgagggcg tcacctccgg cccatcatca tggttgatcg catcgaccag
    26161 catcgacacg cccagcgtgt ccgaaagcag gatgaactcc tgccgcctgt cgttgctgat
    26221 ctggcctgcg gcggtgagaa aggcgatcgc ctggccccat tcggcttcag tcagcctggt
    26281 ttcgcgcacc accgcatgca catggcggat cagcgatgtc atgatctgcc ggaggcgcgg
    26341 atcgggcgca tcggccatcg cggcaatcac ggcttcggtc agttcgggaa aaatcggtgt
    26401 cgtcattgca gcgctcctca ggcccctgcc gtctctggcg gcaagccgga ccaggcccgc
    26461 gcgatcagct cgcggatcgc gccgcgctcg atcgcgcgcg ggttccagta agggtttgcc
    26521 acggcaatat ccgcggcctt gtcgatccct gcctcgggca tccccagcgc cttgagcccg
    26581 ctgggggcac caaccgcgcg gccaagctcg aacaaggcgc gcgccggatc ctctgtgccg
    26641 agcgccgttt tcagcgctgc cgccgcctgc ggcacggcgg gcgcgttgta ggcccaggca
    26701 tgtggcagga cgatggcatg cgtctcggcg tggggcagat tgaaactgcc gcccagcgcg
    26761 tggcacagct tgtgatgcaa ggccattccg accgccccca gcaccgcccc gcacagccag
    26821 gcgccataga gggcatcgct gcgcgcctcg ccatcgccag gattggccgc gatcttcggc
    26881 agcgcgccgg ccagcgcctt gatcccggcc cccgccatca tctgcatgat cggattggtg
    26941 tcgcgggcat aaagcccctc caccgcatgg gcgatcgcgt tgatcccgct ggtgcccgac
    27001 atcgccgccg gcaggccaag cgtgagatcg acatcgtaga gcaccagttc aggcagcacc
    27061 ttgctgctgg aaagcgtggt cttcacgcca ttttcggtct gccccagcac cggggtcatt
    27121 tcggaaccgg cataggttgt gggaatggca acctgcggca aatcggtccg cagcgccagc
    27181 gccttggcca gaccgatggt cgacccgcca ccaaaggcga cgagacaatc ggccccattg
    27241 gcctgcacga cctccagcgc cagggccgtg acatccgtcg gcgtatgcat ggtggcgccg
    27301 tcatagtgcg caacgatggc cgaggcgccc gcgcaggctt tggcggcttg ccgcgcctgc
    27361 tgcggtgtgg acagcagcag cgccctggtg ccgcccacca tggcaatcgc ctcgcccagg
    27421 cgagacaggc tcccctgccc gaagatcaca tgggcagggt ttccgcgata ggtaaaggct
    27481 cgcatatcag gcatcgcttt caagacagct ccttgaggca agacaggtcc ttgagggcag
    27541 tatgcgtatc gccaagcctg ccgggatcgg gcgaaagtcc ggcctcgatc agtttccggc
    27601 cctggacata gtccctggtg gcgttgacgg catcgatggc aatgatcctg ccgcccttca
    27661 gatagatcac cgagaagctg cggttcatgg ggtcgccacg cagcacggtc tggtcataac
    27721 caacggagag cccggctgtt tgcaggcgca aatcatattg gttggaccag aaccagggga
    27781 aggcacggta gggctgcggg tcgccgagga tggatctggc aacggcaagg gcctggtcgt
    27841 tggcattctg gaccgattcg acccgcatcg agatcccggc ccaagggttg gcaaaggccg
    27901 cgcaatcgcc gatggcatag accccgggca acgaggtgcg gcaatattca tccaccgcaa
    27961 cgccgttgcc cccttgcgcg ccggcggcga tcagcggggc cacggccggc acaatgccaa
    28021 tgccaacgat caccgcgtcg gcgggaattt cgctgccatc tgccagcctg accgcagaga
    28081 cgcgcccttc gcccagcaac gcctcgacac cagcgccggt gcgcaagtcc accccgtgcg
    28141 cgcgatgctc cctttcatag aaatcggaca gttccggccc ggcgacgcgc gcaagcacgc
    28201 gcggcagcgc ttcgagcaga gtgacccggc agccgagctt ggtgaggacc gccgccgctt
    28261 ccagcccgat ataaccaccg ccgataacga cgatatccct caccccacca tcgatttcgc
    28321 ccatcaaccg atcgcaatcg gcgcgagtgc gcaccgcatg gatgccgtcc agccccgcac
    28381 cggggcagga cagccggcgc ggatcgccgc cggctgccca gacaagcttg ccaaaaccaa
    28441 aggtcgcgcc atctgcgagg gtcagcaacc gggccacggg atcgaccgcg accacttcct
    28501 ggccgagatg cagctccacg cccttttcgg cccagaaatg cggcgggcgg atgtagagcc
    28561 gctcgaaggt cttttcccgg gcgaaatact ccttggaaag cggcggccgc tcataggggg
    28621 gctcgggctc gcggccgatc atggccaccg aaccggcaaa gcccccctga cgcagcgcaa
    28681 tcgcacattg cgccccgcca tgcccggcgc caacgatcac cacgtcggcg caagtctctc
    28741 ccatctgcat cccctgcccc tttatggctt attgtgtcag cagaaccttc tcgatctgcg
    28801 atttggtcca attgtccggc agatctgcga tgaacaggtt ttcggtgcgg taatggctga
    28861 tcttccactc gcccccaacc tcgcggaaac gcacgcgaac gcggctggag cgcaggagcg
    28921 aagtgccgtt ctcataaatc cacggctgga actgcggcca gatcccttcg gcgccctccg
    28981 ccgtcgccca gacctgaccc aggcagacgt aatgggtgtt gaagttctgg gtagcattca
    29041 cctccgcgta gaaacgccgc atgtgcgccc tgatcgcctc gcgcccttcc atgcggccaa
    29101 agcttgcagc atgcgctcca ccgacacctt cccacaccgc atcttcggtg aagcaatcgc
    29161 caatggccgc gatccgctcg tccagtgtgc cctcatagac cggatagggc acgtcgcaga
    29221 ggaacatgta gcgggcgatc acgcgccggg caccggcttc tgccacaagt tcagagatta
    29281 ccttccagag ttcctcgttg gttttttcgg caggcatgga cattactcca gaaattacag
    29341 caaatatcca atgccatgca gaaaatcatc ggcattgata aaggttacca ctttgcgatc
    29401 gatcctcagc tcgccggctt cgcgcacaat gcgataatcg acatcggcgg cgagcacgcg
    29461 ggtccgatcg tatttgtatt cgacgatgat catcgccgcg cgcaatgcga tggcttgcgc
    29521 gccggcagcc tcgccttcga caaagcggga cagcgtgcgc acggtgcgcg cgggcggcgc
    29581 tgaggacatc gagaagccgg agagcaggcg cttcacccgc atctcgcgca tcggcccatc
    29641 atcataggcg atattgagta cctcggcgta gtcgacagcg gcgacatcgg ccggaatgat
    29701 gtagagcccc cctgcggtcc agagcttgag ccagggcttg tactggtggc gatcgagcag
    29761 ttccgcctcc cgccagatca gccgggctgc ctcctcaagg gtaatcaccg gcgctttttc
    29821 gatcaccgca ctcatgccgt catcatcctt ttccacatct ggtaggcggc gcgcatcccc
    29881 acctcggacg aggcatcgcc ggcattgtgc ccgggctctg gccccccttc cggcgccgca
    29941 accccgcgat tgagcatgat gtagacatca tcgcccgcct gcgcgccgcg ctggacgcgt
    30001 tcccagcctt cggcatcatc gggcgtgcca aagcccatcg gcccctgaaa atgctcgtgc
    30061 agtcgcatgc gcatccggtt ggccgcggcc ggcccccctt tcatgccgat ggccacatgg
    30121 cggatttccg tttcattgac cgcaatcggg cgcaacacgc ggaaaaaagc cattgagcag
    30181 gcaatgttgg ggaacatgtt gaggttgaag cccccacccc cgaccgcgcg cacgatcctg
    30241 cgcacgtcca gatcatcgcg cccttcggcg gccagttcgg cagcaagatc cgcgaaacgt
    30301 tccgggatcg gcgcgtcgag gttttcctcc agatcgatca ggtcgggcat catcaccgcg
    30361 accgaatggc cgttgcccag atcctcgacc cagccacccg aaccgatcag gtccatcagg
    30421 ttttccgtct ctgtgtcgag tgaatcgagg aacgtcttgt ggacgatcgg gaaatgatag
    30481 gagtcggtgg tgttttccaa ctggatcttc cagttgcccg gaaaacggaa cttgtgctcg
    30541 cccagcgttg tcacgccaaa gccggccccc tgcttcatga acaggtcgat ccatttgcgc
    30601 gccggcccga ggtaatccgc cagcggcatg atatcatcgt taaaggtagc gaaaatcatg
    30661 ccattatagg attcggtccg cagcgacacg aggccgaacc gggacttgtc gaaatcctcg
    30721 gcatagccat ccgcataggg caccttgcgc agggccccgt tcatatcata actccacccg
    30781 tgataggggc agacaaagac gctggttttg ccctgcttct tttcgcaaac actcgacgca
    30841 cgatgccggc aacggttgag gagaacatgg atcttgtccg ccttgtcccg cgtcacgatc
    30901 accggctggg tgccgaccca tgaactcctg aacgtattgg gccccggcac ttcgctttcg
    30961 tgcgcaaccc agacccaggt ggatttgaaa attctgtcca cctccacctc aaagatctgc
    31021 ggatcggtat aaagcgaagt atgaacgcgg tcctcgagaa ccagcgatcc gaaatcacgc
    31081 gcgccggcgt tttcgctgac cacacccatt ctcattctcc ttcaatcaaa ccgggtcaga
    31141 agcttgcgac atccttcggt ttatattcag ggtgcaattc catgtcgtaa gccccgtggg
    31201 cattttcctt ctcggcaaaa tagctgacac cgacaatcgg gctgccggac tggaaaatcg
    31261 gccaattgag gacgctgggg cgcaggcgca tccattcgcc ggtaaaaata tagatgaccg
    31321 cttcctgccc attttcgagc ttcagcccct ttgccatggt gccgccaaag cagaagtgga
    31381 agtcgagcac gggaaagcgg tgcggcacca gataggcctt gaaatcctcg tcgcgcagcg
    31441 gggccggttc tgcttcacca tccccgttga acaaggcaat gaactggtgc gcgtcaccgg
    31501 gctgggccgg cggaacatag acgccgatgc gccgtgcctg aaagccgaag ccatgctcgt
    31561 cgagcaaggc ctccagcgcg ggcgccacat cggtataaag ccgattggcg aacgcccgat
    31621 cgtaccgggt ggacggatcg ttggcataag cgacaaaatc ggcaggtgca gaaaagatgc
    31681 cgatctccat cggccctatc aaagcgttga tcctggcgat gtcggtaaag atctcaccgt
    31741 tttccagcct gaggagtgcc acgttaaact tccttccaat gttcagtgca aagctccgcc
    31801 atcaccggac ttgcagagcg caagtccggt tcatcgcatc gtggagctgg agaataagcg
    31861 gccgaagcct agccttacac cgccagttta agcggcgcgc caccttccgc ctcgagcacc
    31921 ttatcgagat accagttgta gaacttgctc atctggactt cgaggcgcgg gtgcagaacc
    31981 gcccgaccat tggtcttcga agtgatgccc ttctgcacca tcttgcaggc gtattcgtct
    32041 tcgagctgca cctggtaggc aaaatcggtc tgaaacttga cagcttcttc cgccccgggc
    32101 gtttccagcg cccatttggg aaacgcctgg caaatcttgc cgcgattggc cgtgacgcga
    32161 ttgccgaatt cgaagatgag ataggtgctc tggtaattgt tcagccacgt tcccatcgtc
    32221 ggaaaaccga acagcacccg cggatcattg tcccaatcga caccttccac gcccggcgga
    32281 gtgccaaagg gctggagata ctcctgcggc acgccatcct tccaccacat gtgataggaa
    32341 cagcaggcct tgccccccca ggcctcttcg gtgttcttgg cgggaatctc accctcgatc
    32401 cggtcatgat ggacacctac gtggtgatag ccttccatgc cggcctcaac cgtaagcttc
    32461 cagttccacg gcgcctcgaa ctcatacgta tagctgtgct gaaaatcggc cactttccag
    32521 gcatcgaaaa tcggatcgat cggggcgtat tgcggtgcca gcggttcggc atccgggtcg
    32581 aaattgatga agacaaaccc ctgccagatc tcgagacgaa gctcgggcat ccggcagttc
    32641 ttcttgtcaa acccggggat ctgctcaacc gagacgccct gtaacgtgcc atcgagattg
    32701 taggtccagc ggtggaacgg gcataccatc agcttggtat tgccccgacc ttcctcgaca
    32761 atctgcgcaa agcgatgctg gcacacgttg atcagcgcac gcagcacccc atcacgcccg
    32821 cggatgatca cgatcggctc gtcgaacatc cgaatggtga aataatcgcc agggttcagc
    32881 gcctcttcct ggcggccgac gatcagccac tgcttcttca ggatgttctc gacttcccac
    32941 tggtaaaaat cttcatcata ataaaacctt ggcggaagag agctgcgaac tccatcaaac
    33001 ccgtcacgaa tgcctttgat ttcgtcttgt gtgaacaaat ttgactgaac cataaaacgc
    33061 tccgcccttg tatttatcgt tccaaaatat acattccgga cgatatattg ctaaacatgc
    33121 ccgacggcat aatatgccat cacccccatg cgtcaaatgc atataggtaa ttacatggat
    33181 tacccacttt aattccgttc ggatgggcag cgttctccgc cggccgcatt acttccaccg
    33241 aatgcaatag agatcgcatt gcgcagccag gcgatcgccg ggtctgccgc aaaacgcttg
    33301 tgggtgtgca cgctgatcgg aatctgcggc agatcgaaag gcaaacccag cacccgaaaa
    33361 tcgcccgcgc tgacgaagcg cgaaacgaca ctggccggaa acaaggcagc cagatctgtg
    33421 ttccgcacca tttccggaag cacggaaaga tggctgagcc gcgccgcaac gttccgctcc
    33481 aagtggagat cggacaaatg ctgctcgatc acaccatagc caggaacccg cctggacaca
    33541 tccacgaaag tcagcccggc aaaatcttca accgtcagtt catcgccagg gaaaggatga
    33601 tccttcctga caatgcagac atgccggtcg tatatcaacg gcaccatcgt atagtcttcg
    33661 gtttcgggga tataaccgat ggcgatatcg gtcttgccgg atttaagaag cgcatgaata
    33721 ctggtgacat cgagattgat cgtctcgatt ttcacattgg gcgcctggct gcgaacaatc
    33781 ctcagaattt ccgaaagaaa ataatattcc gaaacatccg tcatcgccag atggaaggtg
    33841 cggttggctt ccgcaggccg aaatgatcgc tgcccaagca acgcctgctc aacgatctgg
    33901 agcgatgcgg tcaacggggc atagagctgg accgcgactt ctgtcggcac catctcgttg
    33961 cccacacgca caaacagcgg gtcgtcgaac tgttcgcgca agcgcttcag cgtatggctg
    34021 atcgccggct gggtaaggcc aagctgctcg cccgcgacag tcaggctgcg cgcatcccag
    34081 atcgcgataa aggtcttgag cagatttagg tcgaatgtcc gaaggccctt gtgttgcggc
    34141 atgtcatttc tcctggacgg agagggcatg tccacaaccc attcgtcaac caccatattt
    34201 cccgctgtgg atcgcgcaac catcaggcaa gggcccgggc aatcgcgcaa agctgcgcct
    34261 cttcgccttt ccggccgaca agctggagcg aaaccggcca tcccgcaggc cccgccacgg
    34321 gcaggctcag cgcaggatgg cccgacaggt tgaaggggcg aacaaagcgc gagaaggcaa
    34381 ggtccatgcc cgaggagcga acggcagcca cggtgggcgg cggcccggcc agcgttggca
    34441 gcgccagtgc gtcatggtcg tagagcagcc gatccaccgc cgcggtgaag gcggcgcgaa
    34501 cgctctcggc ctcggcaacc tgttcatgcg tcaccgcagc tgccgcgcgc agccgcgctt
    34561 ccacatcagc ccccagcaag ccggtctcga ccagcccgcc aaaagccgcc caggtctcgg
    34621 cgttgatgat gaccaatccg gcggcaaacg cttcctccag caacgccaga tgggctggcg
    34681 ccatcgccag gcccgcgctg acgagggcgc cgcgacaggc ggccgtaacg gcaggatcgg
    34741 catcaacctc caccaggccc acgcgcggcg cgacaacgct ggccggcgcg aagcccgggt
    34801 cgatgatggc catcgcctgc tcgatcatgc caatcgtccg ggcgaaaggc ccaacgcagt
    34861 ccagagaggt ttccgccggg gcaacgccct tgcggctgac gcgaccaaaa gtgggcttca
    34921 acccccagat gccgcagcag gcggcgggaa cgcggatcga gccgcccgta tccgagccga
    34981 tcgcaaaatc ggcaaggccg gcggcaacgg ccgccgcact gccgcttgag gagcctccgg
    35041 ggatcaggtc cgcatagccg ggattgaccg gcgttcccgc ccaatcgttg atcccggaaa
    35101 gcccaaaggc caactcatgc atcaccgtct tgccgataat ccggcaacca gccccaagaa
    35161 catgggcgac gacttcggca ttggtcgtgg ccggcgccac attcgccatt gccctgctgc
    35221 catcgcgcgt gcggcagccg gccacatcga tcgaatcctt gaccgccaca cgcaagccac
    35281 ccgcagagcc cagttcgagc gtttcgacaa agatggtcat ggccgcctca gtgcgcgatg
    35341 cagaccgatt tcagctcggt ataagcgttc acccaatcgg tgccgaactc ccgcccgagg
    35401 ccggactgct tggtgccgcc gaacggcagc gccgggtcga tgaagctgtg ggtgttgacc
    35461 cagacagtgc ctgcatcgag ctggcgggtc agatccatcg ccgccttcag gttctgggtc
    35521 cagatgctgg cggcaaggcc catctcggta tcattggcaa gcgccacggc ctcttcccgg
    35581 cttgccaccc tggtgatcgc gatcaccggg ccgaacactt cctcgcgctt cagggcaacg
    35641 gtctcggcag gatcgaggac gatatgcggg ctgacgaaat agccctctcc ttcgggcacc
    35701 ggcgcccccg cgatcacctg cgccttggcc ctggcatcgt cgagatagcg agtgactttg
    35761 gccttgtgct ccctggacac cagcggcgtc acttgcgcgg ccggatcgag ccctgccccc
    35821 accggcagcg acctgatggc acctgccagc gcctcgacca gctcgtcatg gatcgcctcc
    35881 tcgacgtaga tccgcgagga ggccgaacag acctgcccct ggttgaagaa gccgccgccc
    35941 atcagcccgg gcacggccac cgccagatcg gcatccgcca gcaccaccgc cgggttcttg
    36001 ccccccagct ccagcccggt cggcaccatg ttttccagcg cggcacgggc aatggccttg
    36061 cccgtcgccg tggagccggt gaaggtgatc ttgcggatca gcttgtgcga aaccagcgcc
    36121 gcgcccgcgc cctgcccggt gccggtcaca acgttgaaca catcctccgg cacccctgcc
    36181 tccagcgcca gctccgccag ccacagcgcc gagagcgggg tcacttccga cggtttcagc
    36241 accaccgagc acccggccgc aagcgcgggg gcgaatttcc aggtcgcgat cgccatggga
    36301 aagttccacg gcgcgatgcc cgccaccacg ccaaccggct cgcggcgggt ataggccgtc
    36361 caatggtcgg ggccgggggg catcgacagc tccatcgtcc gcccctcgat cttggtggtg
    36421 agcccggcgg cataccgcag ccattgggcc gagccagcac tggcaaacag gcgggagaga
    36481 tggatcgact tgccctgttc cagcgtttcc agctgggcca gcgcctcgcc gttatcctcg
    36541 atcagttgcg caaagcggaa caggatgcgc tcgcgctcgg cgggcgtcct gtggcgccag
    36601 cggccatcct ggaaagcagc atgggcacgc tcgatggcct cgttcacatc ctcgggcgcg
    36661 gcatcgacga tttcggcaat gggcaagcca ctggcgggat cgaacacggg aatgcgcgcg
    36721 gccgaccggc ccgcgatgcg cttgcccccc accacaatgc ccggcgcgcg ttccagaaac
    36781 gcacgcgtcg cggcatcgat ttcaaagctg ccctgatacc ccaccattcg cctccaaatt
    36841 cgttcaaagg aaaccgggcg aaagctcgac gccagccagt gccccaccag acagttccaa
    36901 cgtcgcctcg ctcagcgacg catgttgcgt aaccaccagg gaatcgcgca gcaactgctg
    36961 caggcggttc gaccgggccg ccgccgccat gcccgcgatg cgataaagca gctggatcac
    37021 ttcggccgca gtatgcgctg catgggtggc gctgaggcgc agcgcgttgc gctgaggggt
    37081 ggacaactca tccccccgca agatcacgtc ccacgcctgc tcggcggcat cgtagtaata
    37141 gaggcggcag ctttgcagca tggcctcggc cctggcgagg cccgtgcgga aataggccct
    37201 gtcggcaagc ttggccgccc ccggcatcag cttcttgccg cccgacatct cggccgcaac
    37261 ctcgattgcc gcgcgcgcca gccccaggtt ggacgcggca tgaacctgcg cctgataggc
    37321 aagcagcgga taacgataca gcggatcgtc gaagattggc tgcgtgccgc gcaggcaggt
    37381 ccagtcttcg gcaataaaat gatcggtcag ctcggtgtcg tagctgcccg tgccctgcat
    37441 cccgaccata tcccaattgg gaatgatctt cacttccgca gcagggctca ccgccatgcg
    37501 gacaaggccc gttccctcat ccgccagacc gatcgcaacc cagtctgccc ccatgcaacc
    37561 actggcaaag cgccagcgac cagagaccct ccagccgcca tcgacccgct tggctggcac
    37621 cgcgggatag agcccgccgg caaaaacctg atccgggctg ctcgcataga tatgccgctg
    37681 cgtttcatat ggcagcgccg cataatacgt gttggccgaa ccaaaacccg ccacccagga
    37741 ggccgagcca tcgacccggg cgatccgctc gatccgctcc agaaacacat gcggcggcac
    37801 cccggcacca ccaaaacagg caggcgtggc agcgcgatag aggcccgcag ccttcatcag
    37861 ctgcaccacg tcgcgaggaa catgcgactt cgcatcgaac tcgtcgcgcc gcgcctcaat
    37921 cacccgcagc agatgatcaa atgcaacttc gttgaatgcc gaggcattta ttttgccatc
    37981 cgtcgaagtt tccagaattt tggtcgccat tacaaatccg ctccacggaa atacactgct
    38041 cacatcttta ttgatccact acgggtgatt aaaccaatga aattgcataa ttccacgcat
    38101 aacgccggat aattctcttt tttaaccaga cttatcggga cactgcgccc cgtcacgcct
    38161 gcgcgaacct ggaaagctgg aatggagaga gatcgatccc gggatcttca cccgccgcca
    38221 gctgcgccag aacagcgcca gcgcccggcc ccatggtaaa gccatacgta aagccggtgc
    38281 cgacgaagag acccggcacc tcgctgacgc cggacaggac aggcagatcg tcgatcgtgc
    38341 tgacgagcgc gcccgaccag gcctcgcgca cctgggcttg cataaattct ggccgctggc
    38401 ggcgcagttc ggccagcaac cggtcgagga gcggctgctg agtctcgggc tgcaggatcc
    38461 gcactttctc gaaggggctc ggccggtcga gccgccaggt tgcaggcgtg gtcagatcgc
    38521 gccagaaatt gctccacgac agccgcggcg caatcggcgg gcccgccgcc agcagcggca
    38581 gcaattgacg gatatatttg aattggctgg gcaggatcgg ggccaccccc tgtattgccc
    38641 ccaccgcata gcccccgtcc agttcccggc gcaccacaac cggtggcagc acgccgacga
    38701 ggtcgggccc acccgcgaag ggcgccaggc tcatgatcga ggcatagccc ccgaacatcg
    38761 gcagttcgat acccaggctg cgcaggaagg tgggggtcca gacaccgccc gccaggatca
    38821 ccttgccggt ccgaaccgtg ccgcgttcgg tgacgatcgc gcaaatcccg cggcccagcc
    38881 gctcaaaccc gcgcaccgcg cattgctgga aaatcagggc gccatgtttg cgcgcgccca
    38941 tcgcgatcgc cggcgcggcc agcttgggtt ccgcaatccc ttcgccagct gcatggaatg
    39001 cgtatttgta agccgatgag gcgccgcaaa gcaggtcgtc gacctggctt gcgcttgcaa
    39061 cccgggcgct caaaccgatg gttgccgcct ggtctatcca atgctgcgcg cccgcaagct
    39121 cttcctcacc gttgaagaat tcaacgatgt catgctcgcg aaagccggtt tcctcccccg
    39181 tcaacgtgtc gaggttgcgc cagcactgct tggacaactc gatcaagggc tgcagccgca
    39241 aatcctggtg cttgccctcg atcagcccat ggttgcgacc ggaggcttcg ccggcaatcg
    39301 cgcccttttc gaacactgcg accgagaacc cgcgtttggc cagatacagc gccgccgaaa
    39361 ccccgatgat gccgccgcca atcaccgcga catcaacgga cgacggcaac gccttgtcgc
    39421 tctcgacagg atgcgcccgg gacaccccgg gcacgagcga ggcctccccc ccggccagga
    39481 tatcccacag ctttcgcgca aaagcagcca tcgtcagccc ttgatgaccg aattggcaag
    39541 atcgctcggc gaaagatatc cggccggggc cgcgatgata tccggaagcc ggttcaccag
    39601 cgtggcgcag gtcacttcca cgccggggaa attgtccgtc acgaaagtca gctccggctc
    39661 tcccctgaaa gtccaggtga tcttttcagt ctctcccggc gcattgacct tctcgacgaa
    39721 ctccatctcg agtttgatcc cctcgcgggt ttcgatctga tagatttccg tcacaccggc
    39781 agtgcggccg ggcaggatgg tcctccccag gctttcggat acgatctcgc gctcggcata
    39841 gatcggttcg tggcgcacgc tccgctcggt tggcgtccac cccagctttg ccaccatcgc
    39901 ctccagagcg atgcccgaaa aggggatcag ggccttggct tcgccctcgg cggcctgttt
    39961 tgcatagccc tcgggcgtga ggtcgatcgg atattggccg atcagggccg ggccgtagac
    40021 atcgagattg acgttcgagc ggcaatggac ttcgccgatc cgatgcaccg cgcctgtgag
    40081 cagggccggc agattgagcc agaagatatc ctgcaccccg gttgccgcca gggtggcgcc
    40141 attggcccgc gcgacagcgt tgagcgcctg gccgatcact cctgcggtcc cggggaagaa
    40201 ggcttcttcc gagatggtcg ccacattcac cccatgcgac aagctgagtt cggcaatcgg
    40261 cgacagcgat tccagatcgg aactggtcgt caccaccacg atatccaccc cgcccctggc
    40321 caggattgcg gccagatcgt tggtcaccgc cacccctgcc ggcaccggca atccggccag
    40381 cgttccgata tcctcgccct gcgacgaggt ccggctcacc acgccgacaa tatggatgcc
    40441 gttttcaatc atataccgcg cggtcagacg gcccatgaag ccaaccccga ccagaaccgc
    40501 attaatagcg ttcatcgttc cattcccttg acaacaggtg caatcatccg ggccagccca
    40561 cacggcctag cccaaggttc gctcatacaa tttataggca tcgaccagcg cctcacgccc
    40621 gcgaacgcca atgggaacgg caggggtctc gatccccgcc accagctcga agcgcgaccg
    40681 gtcaccatgc agcgcctcgg cgataagctg cccgttcagc accgcccagg cgacgttgga
    40741 ggccagcgtg taataaacct tggggctggc ctcgccgata tgcggcagga atttgaggtc
    40801 ggccgccccc ttgccctccc aggcataatc gatctgcaca tccgcaagtt cgggatagat
    40861 cgccagcatc cgcttgcgca gcacatgctt gcgcttttcc tcagtatcca ggctgatcgg
    40921 ggaaacgctg ccgaaaacca gccgattgtc cccgctcagg cggtagaaat cgatgaacat
    40981 gcgggtgtcg ttgaccgcca gccgttcctt catcaacgca gcggcgcgcg cgcttcccag
    41041 cggctctgtc gcgatcaccc aggcataggc gttgaagatc ttctgcgcga tgtgccgatc
    41101 gatgcgatgt atattggcat tggtcgcgat gatcagcttc tcggccatca ccgtgccggt
    41161 agcggtcctg actttgacgc cagcgccaga ggcatcgtag gacagcgcat cgctatcctc
    41221 gaatatctga acgccggcct gagcgaccac cttggcaaag cccaccgcga attgcagcgg
    41281 attgagatgg accatgtcca tatcgaaaaa accgccgtaa taggcttcgg tgccgataag
    41341 atcggccagt tccgcacgat cgtaatattt gacgttctta cgatatccgc gcgccaggaa
    41401 cttttccgcc cccagccgca aatgttcggc atgtttcggc ttgtgggcaa catggccaac
    41461 gcccttgcgc atatcgcact cgatggcgtt gcgcgcgatg atctcgccga ccagccggca
    41521 ggcctcgctg ctcaattccc acatgccgct ggcccggtcc gcgccgatcg gcttctcgaa
    41581 atcgtccgga tcgtggccga acaggctttc catgtgcccc ccgttgcgcc ccgaggcgcc
    41641 ccagcccacg cggttgcgtt ccaacagcac caccctggtg cccttctgcg ccagatgcag
    41701 ggccgttgac aggccggcaa agccaccgcc gagcacgcag acatcggcgc ggatatcccc
    41761 ggccaggcgc ggataatcga ccagccgcga tgcgacactc gctgcgtaga aggagtttac
    41821 atgctgtgtt ttcgtcatgg ctctatccgt ctttccgcgg ttgggggcgt caaaattgcc
    41881 aatccgccca ttattattat ttaattacaa tgtattgatc gcaattccgc caaagctgcg
    41941 cgcataaagg catccgcatt cgccgagtcc gaaatgtcgt tgatcatccg ctgggacagc
    42001 atcaccagca cgctgtctgt ggcagggtcc atgaacagcg aggtgccggt tccgccccac
    42061 cagccaaagc ggccgggatg cggcgagaca tggtttgcgg cctgcaccac gcacaggccc
    42121 agcccccagc ccagcgcatc ccagaagccg ggaaactgcg gcgaccgctg cttggcctgc
    42181 gggccaacat ggtcgcgccg catttccgca acaaggcctt gcgcaagcag gcgccgcccc
    42241 tggaaggttc cgccatccag cagcatccgc ccgaatttgg cgaggtctgg cgcggtggaa
    42301 atcagctcgg atccgccctg gtccatcggc cgtgcctgcg tgcggcgcgg cgcatcggcg
    42361 atctggtaat agcgcccgcc ctgcgcggca tagaggctcg ccagccgccg ttcacgcgcc
    42421 gggtcgagcc ggaagccgct gtcgtgcatg cccaggggtt caaaaatgcg ccgcgccatg
    42481 aacgatccca ggcgctcccc cgcaacccgc tcgatcagga taccggcgac atcgatgccg
    42541 atatgataga gccagcgctc gcccggctgg ctgaggagag gcagcgagcc gatgcggcgc
    42601 atatattcat caccgtctcc tgcgaaattg aacgccgaaa tgtgcagcgc ctgcaagcgg
    42661 gacgacacgc tggaccaccc ggcataatcg aaccccaggc cgaaggtctg gctcagcagc
    42721 ccgcgcaagg tgactggccc tttcgcgggc accgtatcgg caagcggggc agcggccgtt
    42781 cgcagcaccc tgcgatcggc cagttcgggc agccaccgcc ccaggtcctc gtcaagcgcc
    42841 agcaggccat cgtccaccag catcagcgcc gcaaccgccg tcacaggctt gctgaccgag
    42901 gcgatgcgaa acagcgtatc aggctgcatc ggcgcgccgc ttgccagatc cttgcagccg
    42961 gcggcatgga tccgcacttg cccggaacgc tcgacaagcg cgaccatccc gggtgcatgg
    43021 ccatcacgga cccggctttc aacggcctgg atccagtcgg cgccatcggt cagcgaaggc
    43081 atcggctcaa tccaggctgg agcgcaatac aagcgcgcag atcgcggcgc cgaagcaggc
    43141 ggctgcgctt acccagccca cggccgccag ggagatccgg tccgcgatca ccccgcccag
    43201 cagcgggctg agcgcaccgg cgaaataaac cgcaaagccc gacagcgtta ccgcgcgccc
    43261 ctgattgtcg agcgcggccc cggcgctgaa cagatagggc atcacgaaga aggccgacat
    43321 gccgtaaagg aagatgcccg tcgcgaacca gaccatcgcg ccgctcgcgc cgatcgacag
    43381 ccccgccagg cagcccagca aaatgccaag cgcgagcggc aacgccactc cgaacctgcg
    43441 atcgagaaaa ccggccaaca gcgaaccgat gatgttgaag acaccggaca ggccaagcac
    43501 gatcccgacg gtatcgacat cgaacttcct gatcgccgcg acactgagcg agaaactcca
    43561 caacatctgc aggccgccgt tggccagcgc acacatcagc aacagcaccc acagcgcgta
    43621 agccggtttg ggctccagct catgctgctc gaactcggcc gtgagcgaag gcctgggaca
    43681 gccaaagacc gcaagcgccg ccgccgccgc caggcacagc gcaaccagca gcccatagat
    43741 cggcacaaag ccgtatttga gcacagccga tggccagacc acaaagccaa ggccataaac
    43801 aacgctggca acgataaagg acgcgctcat cacccgcaca ccggcatgat gctgcgcagc
    43861 ccaataagcc gccgaagcat agagaacgcc gctgctgagg cccgccacga tccggcagac
    43921 aacgaaccag gccacaccat aagaaaggca agacaggata ttgccggcaa cggtgatcac
    43981 cagagcccag aagcacaggc gcgccacgtc gatgcggctg aaaacattgg ccagccccag
    44041 agcggtgaag gccgagaaaa tcacctcggc cgtgacgatg ccaccagcgg cagcttccga
    44101 cagccgatag atgcgcgacg ccacctcgat cacgaacggc gccacgaaca atccgcaggc
    44161 cccgatcgtc aacaacagcg gacaaaggat ataggcggcc caactcgacc caaaggtccg
    44221 gggagattcc atcgctatac ttgccatcga tatcacctcg cctctgtcag ctgccgccac
    44281 ccgggctcca ggtgcccttc tgaacgatca gccatgcacc atcgatcttg tggaggatga
    44341 agacgctgcg catgtcatgc ccctccagcc cgttttcctc gatcgcgaca ttggcaagcg
    44401 tgccatactg atcgacccgg acgatcctgc cctcgaaatc ggggctgctc tcatgcgtcg
    44461 gcggagcctt gcgcatgtaa tcggccacaa aggcgcccgc attggggatc accatgaact
    44521 ggccgccgaa atgccccgtc atcaccgctt gcgcgtgaaa ggcgctggcg accagatcgg
    44581 gatcggagtc gcgaatgcca ttgatgtatt tgaatacgac cgtctcgatc gcgccacgtt
    44641 cgtcactcat tgcctattcc ttccactcgc aacctggctt catcctcatg gacaagatcg
    44701 tcgatcgttt cgtcgtaaac gaacttgttg ccgccgattt tcaaataccc ctgaaaagaa
    44761 ggcagctctg cggcgccata ctggttggca atcaagtgaa tgtgctcctc cccaaccttg
    44821 tccagcgcca gaaatccgtc gatatccatc aggaaatcgc ggtgcggtat ttcatggtcg
    44881 ttgaagaagc gggatttggc gatggccttg gcttccactt cgctcttcgc cgcgatcact
    44941 tcaaacgcat ggcgctcgaa gctttcctcc cggaaatacc cacccatatt gacgaaaaac
    45001 agcctggcct cgccttgcga aggccccgaa ccgatggtca catcatagcc atccacccag
    45061 ttgacgcgac ggatgacatc gatgtgcaaa agctcctggt ccgcaaacca acgatttgct
    45121 attcccgcaa aggcttcttc cggtttttcg acgacagcga actgaacatc gtgaatttcg
    45181 atattcgcgc cgggcgccgt acctcccacg aagaacatgt aaagattttt catcttacat
    45241 ccttctctca agaattatgc caacggttcg ggaagccaga gctctcctag aacttcacct
    45301 tgccagtcgc gccgaaggtg cgtggcggcg cccactggat gacatcgccc tgatactgcg
    45361 agaacagcgc aaccgcataa tcggtgttca gcaggttctt gccccagaga gtgatgccga
    45421 attttccgcc cttcgacgtc cagcccactt gcgcccccag caagctatag gcagcctgct
    45481 tcaggcggcc atcaggctcg gcattccaac cgctgttgta attgtaggtg gtgttgatct
    45541 ggaaatcgcc cacttggctt ggaatggtat acgtcgccga agccgtaatg gtaagtttcg
    45601 gtgcaaacgg aacccggttt cctgtcgcat cgccggtcga atatgccgta ccccctcctt
    45661 ctgacagggg gatcgatgtg gtgtaatcgg cattcgggta ataggtgaag gtcgcatgca
    45721 gcaggcttgc ccccgcatgc aggctgaagc ggggcgtcaa aaccgcatcg gcggcgatgt
    45781 cggcaccata gaagcgggcc cttgccccat tgctgatatc caggattccg gtctggaagc
    45841 tttgcacctg gatattgctg taattgtaga gaaaccccgc gacattcaac cgaaaccgct
    45901 tatcccttgt ctgagccttc acgccgattt cataggcgtc gagcttttcc ggttcatatg
    45961 gcaccacctc atatttgccc gttacggcat tataattcgt gatgccgggg ttaaacccgc
    46021 cacttttata gccacggtta taggaagcat atgccagaat ctgcgggcta aagctgtgat
    46081 cgagcgacac ccgccaggtt ggcgcatgaa accacttgtg cccgctggcc gtcacgtcag
    46141 gaacggtgga gccgacctca agcgtctggg agtcgagaaa agccgaccgc cgctccgtcg
    46201 taaagcggat accgccggtc aggtgggttt cgggcgtgat cgcccaagtg gcctgcccat
    46261 aggcagcaaa ggaatccgtc ttgatcgtcg catagtcgat cagatccgtg accggatagt
    46321 aaagcccccc gtagagatgg accggatcgt attttgcggt ttcgtggaaa taatacaggc
    46381 cggcagtcca tgtcacagct ccaccggatg gcgacgagaa ctgcaactcc tggctgaatt
    46441 gctcatgcgg ctcgttcaga gaaatattct ccgcataaga cggattgggc gtgagcgctc
    46501 catcttccac cgtaagggat tcggttctct ggaaggcggt gatgctctcg atatgaacct
    46561 tttcgagatc ccattcgctc gtcaacgaca cgccatagct gcgaatgtag ccatagggat
    46621 cgtaatagcc attgagatca tgcggccgaa gattcgtgcc cgtgccgcca agcgaaacgg
    46681 tgcccggcgc cgaggccaga accgggtcat accggacata gccgtagttg ccggcaagcg
    46741 tgaacttcag attgctgccc ggttcccaga gcagtttcga acggatgaag atcttgcgcg
    46801 tcttgtcgat gtctttgccg gtcgcctcgt tgcggccgta gccggaggcc tgatcgctga
    46861 aatccaccgc gacattcgcc gcgaggtttt tcgtcacttc cccggtcagg taggcactgg
    46921 cggtcagcgt ttcataggag ccatacccca gggtggcatt gccgctggcc gtgtgcgagg
    46981 gatcgagggt gcggatctgg atcaggccgc ctgtggcatt gcgcccgaac aacgtgcctt
    47041 gcggcccgtt gatgacctcg accgccttca cattgccgaa atcggcagca ccgcccacca
    47101 tcgaggcctg gtaaacgcca tcgatataaa ccgcgaccgc gctttcgatg ccaggcccgg
    47161 tcgccgttgt gccgatcccg cgcagatgcg gctgagacca gccgccttcc tcggcaaacg
    47221 tgaggctggg cgccagcata ttcagatccg caacgctgga aacattcgat ttctcgagcg
    47281 tttcggccgt ggccgcggtc accgaaatcg gcactttctg gagattttcg ctgcggcgct
    47341 gggccgtcac ggtaatctcg cccacggcag gcgccgcctc ggcggtcgac gtcgcggcag
    47401 tctcggcaac cgcagtctcg gcaatggcag atgaggacca ggaaacgagc atggtagcta
    47461 caagcaccgc cgcgacagag actccatccg acagcgaaga tcgcgtaatg tttttcattc
    47521 ttccctcata ttttgtttta ttgaagatgc agtgtgttcg gcgactccaa ccctcgagca
    47581 cataagtctc ttcggacgca atacggaccc ttatttacaa gggtatttgc ccgaaattca
    47641 cgtccgatcc tccacaaaca tcattacatc agggagatgg gcgtgagaac atcgcttttt
    47701 attattctgc ccattcgcac gatcgataca gcatccagcg cgtttacaga tcactttcgc
    47761 agcgacacgg tttacgcatc caaataaatg ccattaattc tctgcattac atatattttg
    47821 agaacgctgg gacgcgacat gccaaatcgc cggacggcta cagctccgac aaaatgcgca
    47881 tcgcagataa gtttttcggc ttcctccaaa tattccaccg aaaccagtgc ggataattta
    47941 cagaaattca ctcccgcacg gcaacgcatc gtcacaatac gtcacaatac aaggaaaaca
    48001 ggagaagcat cggaatgcat ccatctgaaa acacacgctg acagatacat tccgagagaa
    48061 atatattttc aaagcaatat attgctatca agcgaaggat ttttggatat ccgccaccag
    48121 cgcgctgatc tgcgcccgcg ctgcagcgag ggattcctcg aaatagtcct tcttcgagag
    48181 ggccccatag agcgatacag catcgatatg cgccacacca aggtgagata agggctcgcg
    48241 caaagcggcg atggcagcac cggcgccgct ctccacatcc ccgctttccc gggatcctgc
    48301 cgaagtggcg atcaccaggc gcttgcccgg cgccagcagg cccttttccc cgccggtgac
    48361 ggaaaaggtg aggcccacgc gcacgacatt gtcgatccac gccttgagct gggccggcac
    48421 gccgaaattg tagaccgcga ccccgatcac gatcacatcg gcgttcagca gttcctgcgc
    48481 cagcacatcg ctttccgcca atgcggcgcg aatggcatca tcatgcgcgg ccggatcgga
    48541 aaacgacccc ctcaaccagt cgacagtgat cgcggccggc ggattcacgc caacatcgcg
    48601 ctcgacaatc acgtcgttcg gccgggtttc gcgccagcga tcaagaaaat atccggtaag
    48661 ttcacgcgaa tgggaaccgg caaactgttg atcggatcga ttcggctgcg gactggaact
    48721 cacgtgaagg atattcagcg gcgcattcga cacgggtctt tcctttccaa gtcatcccgg
    48781 gaacaataaa aaatctgcct gcccggatcg gatggcgatc gggaaaagac cttcccccgc
    48841 ccgcctccgc cgtcgctttt gcggccctgg ccccgaaggg ttcgagtccg tccaaccgcc
    48901 aaaatggcct gctccacata aaacagaatc gaatcattag tgtcaagatt gtttcatttt
    48961 tttcgaagtt gccccgcttt cgcataagca gataccctca gcaccaaccg caaaacccgg
    49021 ccacatcagc agaaagcacg ccgcccgctc gcgtgatcga tcggcgttga gggcgggcaa
    49081 tgtcgatgca aacgccgcac gcccatcctg gccaggatca attgaggcct gggcctaggc
    49141 acggcatctt tgccgcccta ttcaggtggt tatcggcccg cccttccccc atccgggccg
    49201 gcccgaagaa ggagattgcg atgaagccgc tgatcggacg cgacacccgc ctgtgcatgt
    49261 cgctggcggc aaggccgggc aatttcggct cgcgcttcca taaccggctc tacgagctgt
    49321 gcgggctcga ttatgtctac aaatccttca ccaccacgga tctggccggc gcgatcggcg
    49381 gcatccgcgc gctggatatc cgcggctgcg ccatctcgat gccgttcaag gaggacgtga
    49441 tcgccctgct cgacgcgctt gccccctcgg ccagcgcgat cgacagcgtc aacaccatcg
    49501 tcaacgatgc cggcgtgctc accgggcaca acaccgatta tggcgctgtc gtcgatctgg
    49561 tggccgggct cgacagggcg ctgcccttcg tgctgcgggg cgcgggcggc atgggcaagg
    49621 cggtggctgc cgccctcagc gacagcggct ttcgcgatgg cacgatcgtg gcgcgcaatg
    49681 ccgcggccgg gcaggcgctg gcggatcttt atggctatcg ctggcaggcc gcgcccgcag
    49741 gcacggcagc gctgctgatc aacgttacgc cgctgggcat ggtgggcgcc gatgccgatg
    49801 cgctggcttt ccccgaggcg atgatcgcgg cctgcgaggt ggcgttcgat gtggtgcaat
    49861 atccgccgca aacgcctttc ctgcaggcag cggcgcagat gggcaagcgc tgcatcaccg
    49921 gcaccgaagt ggccaccttg caagccctcc agcaattcgc gctctacacc ggcgtgacgc
    49981 catcgcccgc gcaagtggcc gaggccgcgc aatgggcccg cgcccatccc tgatctaaag
    50041 ctggctgtcg ggcagcaggg cgcgggtcat gccgacaagg atgttgagca gctcgcgctc
    50101 caccgcattg gcggtgggca ggcggcacag ggccaccaga tcctgcagcg cgcgcagtgc
    50161 atcggcgcgg cactggtgca ggtttgccgg ccgggcgcgc ccttgcgcgg cgcatagcgc
    50221 gcccgcccgc agctgcacct gggccagggc ggcgcggccg gcggaaccca gctcatgcga
    50281 catcgtcccg atctggatga ggttgaggcc aatgcgcacc tcacgcaggc cattgctgag
    50341 cgccagcgca tggcccggca ccgcttccag ctgggccagg cgcggcgcca gcagcgcggc
    50401 gcggtccacc atcaggctgt tccattcctc acgcgttacg ctggaacgcg ggctggccag
    50461 ccgcgccaga tcgcgccagt tgcgccgcag gatgcgcccc accgcccgct ccacgccgat
    50521 tacccgcagc atggcggtgg tgaggatggc cgccgccgcc cctgccagct gggcgatgtt
    50581 gttgttgagg aaagcggcga aatcggcgtt cagcgtgtct gtcagggcaa tcgcgccgca
    50641 aaagcccacc accagcgtca tcatccgcgc atagagcgca ggcacgccca gaaacacccc
    50701 cagccccacc agcgtgggcg ccagcgccgc catcagcatc tcgaacgtat ccagccgggg
    50761 caggatggca aagagataga gcgcggcgat cggcaggctg agcgcggtcc acaccagcca
    50821 gctggtctgc gccgggcggg gattgtcgag cgtggcgaag aagctgcaca ccacagcgca
    50881 gatcatcgcc atcaccccgc cttcgggcca ggcggtgtag atccagaaca ggcaggagcc
    50941 aaagacgcca atccccgccg ccaccgccga gcgcagcgcc agcccgccat cgcggtgcca
    51001 gcgccggcgc gtgcgctggg ccagcagcgg gcgcagcgcg gcgggcagcg gggtggccgg
    51061 gtgcagcgtg tgctccacca gccatttcgc cgcctcgatc cgctgcacca gctcggccag
    51121 gcgcgcgtgg aggttcagct ccagcaggcc ttcccagccc tgcgggtgca gaccggcgtg
    51181 ccaggtggcc agcgccgctt cgatatccgc ccgcgaggcg gaaaggtctg ccggggcctg
    51241 gctggcccaa tcgcccagcg ccctcagcag ctccgccagc gccggtgaca ggccgcccag
    51301 ccggtggatc tgggcaatgc ggtcctcgat ggcgtcaacc agcggcagca gcagcaccag
    51361 atgatcccac atcgccatga tcgcatcgcg gcccagcacg ccattgtccg gatggaagcg
    51421 caagtgcgtg gccagcaggc gcagctcggt catcgctgcg gccatggtgc cgggcggatc
    51481 gggcggggcg ctttcctccg gccggcgcag cacatcggca atccagcggt tcatcccgcc
    51541 acgcacaccg cggatgctgg cggcaatcgc ctgctcgaaa tcatgcgcgg ggatcagcgt
    51601 gtgcagcagg ctggaacagg ccacgccaat cgagatctcc tgcccgcgca gcaccgccag
    51661 gtcgaagatc cgctcgggcg tgtccaccgc cgggaaggtg atgagcgcag cggtgtagcc
    51721 ggccagcatg aacagatagg cgcgcggcgt gcgatcaagc agcgaggcga acaggcagca
    51781 gcccacccac agcgccatca ccagcgacag caggatcggc tcctgcacga acaggggcac
    51841 gaacagcacc gatgccgccg ccccgatcag cgtgccgcca atgcggaaca cccccttgga
    51901 cagcgccgcc gccgccagcg gctggctgct gatataaacg gtgagcatcg cccaataggg
    51961 ccggtccagc ccgatcagga aggaaatgcc cagcgccagc atggccgccg caaaggtggt
    52021 gagcgagaag gtgatttccg ggcggccgat gcgcggcatg ctcaacgccc cttgcctgcg
    52081 cgcacccgct cttccagcag gcgcagcacg cgcagcgtgg tggcgatatc ctctgccggc
    52141 agatcgccca gcatggcgcg gcggcgctcg gtcagccggg cttccatatg ggcggccagc
    52201 ttgcgcccgc gcggggtgag gcacacgccg cgcgcgcgcc ggtcggccgg cacggcgcgc
    52261 cgctcgatca gcccgccctg ttcggcgctg gccagggcgc gggccagggc ggccggatgc
    52321 acgccgatct cttctgccag gcgcgcctgc aacacgccat cgcccagccg cgagacgaac
    52381 agcaccggcg ttgccaccgc caccgaaacg cccgattgtg ccagcgcggc cgcggccacc
    52441 tgctgccagg cccgccgcgc cggctggatg gcgttggtca gggcatacag ccgggcatcg
    52501 ccgggcgcgc ccctctccga cattgttgac atggcaacga tctagcccgc cgcccggcaa
    52561 ctggcaaggt ccgccttaat ggccctgctc aggggcaggc cgccgccttc tgcccgcagc
    52621 ccagcgcgcc tgtcaccacg ccgatcgtat cggccaccat cgcttccacg ccgggggcgg
    52681 tggggtggat gtgatcgggc agctgcaggc ccggccgctg atacaccgcc ttcaggaaaa
    52741 agggcaccag cgcggcgtga tgcttgcgcg ccagctgggc gaacatggcg ttgaaggggc
    52801 cggcataatc gggccccatg ttgggcgcgg ccagcatgcc cagcaccacc acgcggatgt
    52861 gcctgtcctc cagcctggtc aggatcgcat cgagattggc ctttgcctgc gccaccggca
    52921 ggccgcgcaa catgtcgttg ccgcccagcg agagcagcac cagatcaggc ggcgggttct
    52981 ggctggcaag ggtgaaatcg aggcgggcaa ggccatcggc ggtggtatcg cccgagacac
    53041 cggcgttgac cacctgcgcg ttgatgccgc gcgcgcgcaa ggcggcctcc aggcgcggcg
    53101 gataggcctc ctcggtgcgc aggccatagc cggccagcaa actgtcgccc agcgcaacga
    53161 tatgcaccgc agggccctgt ggcgcggccg cctgcgcagc gctgctggcg cggctttcgg
    53221 gcatcgccgc ttcctgccgc tggcacccgg ccagcgccag cagcagcatg cccgccataa
    53281 tgcccttgcc caagcttcgc atcgcacacc ctttttctct ggcggcccat cttgccaccg
    53341 cattgcagca tacccataac cgatatcgtg acccaccccc cttctggcaa gagcgccatc
    53401 ctcgcccgca acctcaccct cacgctgggc agcggggggg aggccaccga gatcctgcgg
    53461 ggcctcgacc ttgaagtgcc ccaggggcag acggtggcgc tgctgggccc gtcgggcagc
    53521 ggcaaatcct cgctgatggc cgtgctcacc gggctggagc gggcgagcgg cggcagcctt
    53581 gccgtcgccg gcgcggattt tgcggcgctg gatgaggatg ggctggcgcg ggcccggcgc
    53641 gggcgcatcg gcgtggtgct gcaggccttc cacctgctgc cgacgatgac cgcgctggaa
    53701 aatgtgatga ccgcgctgga actggccggc acccccgatg ccgaagcgcg cgccgccgtg
    53761 gaattgcagg cggtgggcct tggccaccgg ctgcaccact atcccgccca gctttccggc
    53821 ggcgagcagc aacgcgtggc gattgcccgc gcgctggccc cgcgcccggc gctgctgttc
    53881 gccgacgagc ccaccggcaa cctggatgcc gccacgggcg ctgcgatcat cgagctgatc
    53941 ctggcccggc gggcgcaaac cggcgccacc ctgctcatca tcacccatga cgaggcgctg
    54001 gccgcgcgcg cgcagcggat catccgcatt gccgatggcc gcatcgccgc ggacgagctg
    54061 gcgtgagcct ctcctgggcg gcggcctggc gcattgcccg gcgtgaactg tctgcccggt
    54121 tccggggctc gcggctgc
//
LOCUS       DASRYE010000042         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_178113,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000042 DASRYE010000000
VERSION     DASRYE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178113"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /gene="addB"
                     /locus_tag="VFF98_02505"
     CDS             complement(<1..2185)
                     /gene="addB"
                     /locus_tag="VFF98_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HZU62528.1"
                     /translation="MVEGRASARAGGPAHHSPAVYSIAAHRGFADALVAGLIPRYGAG
                     PMGLAGLTLLVPSQRAARTITEAFIRAMGAGGRVAGLLLPRLVAVGDLDLDEALGPLF
                     DPLDAEGIAPAADPTRRWLRLAGLLAAIEGPEVAGGAARLRRAREAARMLDRLQVEGI
                     APDSLLDEEVIALVGEQAEHWRDATRDFLKLHALWRFDCAGRGEVDPPERRNALFAAA
                     AARWQAQPPAGPIVAAGVTSASPALARLLRVVAGLPDGAVILPDFDLAMDEEVWAELG
                     LAGAPAEAGGPAFGSGDAVTHPQYHLKLLLNRMGVARTEVQPWHRAGLAAAPPERARA
                     LSALFLPPQASARWVDLPEPARRLAGVRLMPTAGPAEEARALAILIRQALEEPGKRVA
                     LVTPDRALAGRVVAQLGRWGIAADDSAGQPLTRKAAGRLALLLAEVAAEGAPPVPLIA
                     LLSHPLVGRALAEMPRGQWLEQARALDLALRGPRPDAGLAPLRALVEQISRRQGGEGA
                     ILGWWQAVEAALAPLFALPPEGPLADQIDALILAAEALCGEAVWAEADGRLLGGLLED
                     WRAAAAAEDTVLPLRDLPAALRDGLEEVAVRPAWGGHPRVAIYGLLEARMARADLMLC
                     AGLVEGVWPGAPAPEPLLPPALLRHLGVPGADFRIGLAAHDLAACLGAPQVVLSWAAR
                     DASAPVIPSRFVLRVEAMLGADLAARHHERQALEWAAAIDSRPEAP"
     gene            complement(2178..2900)
                     /locus_tag="VFF98_02510"
     CDS             complement(2178..2900)
                     /locus_tag="VFF98_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZU62529.1"
                     /translation="MSALVSDTAMVMAAGLGKRMRPLTATRPKPLVKVAGRALIDHTL
                     DRLAEAGVARAVVNVHYLADQLEAHLAQRKAPAVVISDERAALLETGGGMARALAQGL
                     LPDPFFCLNSDNVWLDGPRNVFAELSGAWDAQRMDALLLMVRHTGAHNYHGQGDFRLD
                     PEGRVARRAERRVAPFIYTGIQIVSHRLLRDCPAGSFSTNRLWDRAMAEGRLFGLVHT
                     GEWCEVGAPGHIGPTEALLTHG"
     gene            complement(2897..>3791)
                     /locus_tag="VFF98_02515"
     CDS             complement(2897..>3791)
                     /locus_tag="VFF98_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZU62530.1"
                     /translation="RIHKASQSAMLMHAPPPQEDPRPFLAVGGWLAAQGLRPPAVLAE
                     EAGAGWVLLEDFGDARLRDVLDETPALEMPAYAAAIDTLVALRGKAPGPFPPYDMATY
                     LREARLFVEWFCPAQGLAVDAPGWEAAWQEALAPLMARQQPGVAVLRDYHAENIMLLA
                     GDPADQGLLDYQDALVGHPAYDLVSLLQDARRDVAPAVEAAMLARYLAAAGLAGEAES
                     ADFLADYARLGAQRNAKIVGIFVRLWQRDGKQRYLALVPRVWAQMERDLAHPALAPVA
                     RWFEANIPAAIRAAHGGSLAA"
BASE COUNT          571 a         1481 c         1295 g          444 t
ORIGIN      
        1 gcggggcctc ggggcggctg tcgatcgcgg cggcccattc cagcgcctgc ctctcgtggt
       61 ggcgggcggc aaggtctgcg cctagcatcg cttccacccg cagcacgaag cggctgggga
      121 tcaccggcgc cgaggcatcg cgcgccgccc agctgagcac cacctgcggc gcgccaaggc
      181 aggcggccag atcatgcgcg gcaaggccga tgcgaaaatc cgcccccggc acgcccagat
      241 ggcgcagcag cgccggcggc agcagcggct ctggcgcggg cgcccccggc cacacgcctt
      301 ccaccaaccc cgcgcacagc atgagatcgg ccctcgccat gcgcgcttcc agcaagccat
      361 agatcgccac gcgcggatgg ccgccccagg ccggccgcac ggccacttcc tccagcccat
      421 cacgcagggc cgccggcaga tcgcgcaagg gcagcaccgt gtcctcggcg gcagcggccg
      481 cgcgccagtc ttccagcaag ccgcccagca ggcggccatc ggcctcggcc cacaccgcct
      541 cgccgcacag ggcctcggcc gcaaggatca gcgcgtcgat ctggtcggcc agcgggccct
      601 ccggcggcag ggcgaacaag ggcgccagcg ccgcttccac cgcctgccac cagccaagga
      661 tagccccttc cccgccctgg cgccggctga tctgttccac cagcgcgcgc aagggggcaa
      721 ggccggcatc gggccggggc ccgcgcagcg ccagatccag cgcgcgggcc tgttccagcc
      781 actgcccgcg cggcatttcg gccagcgccc ggcccaccag cggatgcgac agcagcgcga
      841 tcagcggcac cggcggcgcc ccttccgcgg ccacttcggc cagcagcagc gccagtcggc
      901 cggccgcctt gcgcgttaac ggctggccgg cggaatcgtc ggcggcaatg ccccagcggc
      961 ccagctgcgc caccacccgg cccgccagcg cccgatccgg cgtgaccagc gccacccgct
     1021 tgcccggctc ttccagcgcc tggcggatca ggatcgccag cgcgcgcgct tcctcggccg
     1081 ggcctgccgt gggcatcagc cgcacgccgg ccaggcggcg cgccggctcg ggcagatcca
     1141 cccagcgggc cgaggcctgc ggcggcagaa acaacgccga cagcgcccgc gcccgttccg
     1201 gcggggccgc ggcaaggccg gcgcggtgcc agggctgcac ttcggtgcgc gccaccccca
     1261 tgcggttgag cagcagcttc aggtggtatt gcggatgggt gacggcatcg ccgctgccaa
     1321 aggccgggcc gcccgcttct gccggagcgc cggcaaggcc cagttcggcc cacacctcct
     1381 cgtccatcgc cagatcgaaa tcagggagga tcaccgcccc atcgggcagg cccgccacca
     1441 cccgcagcag ccgcgccagc gccggcgagg cgctcgtgac gcccgcggcc acgatggggc
     1501 ctgccggcgg ctgggcctgc cagcgggcgg cggcggcggc aaacagggca ttgcgccgct
     1561 cgggcgggtc cacctcgccg cggcctgcgc aatcgaagcg ccacagcgcg tgcagcttca
     1621 ggaaatcgcg cgtggcatcg cgccagtgct cggcctgttc gcccaccagc gcgatcactt
     1681 cctcgtccag caggctgtcg ggcgcgatgc cttccacctg cagccggtcg agcatgcggg
     1741 ccgcctcgcg cgcgcggcgc aggcgggcag cgccgccggc cacttcgggc ccttcgatcg
     1801 ccgccagcag gcccgccagc cgcagccagc gccgggtggg atcggccgcc ggggcaatgc
     1861 cctcggcatc gagcgggtcg aacagcgggc ccagcgcctc gtcgagatcg agatcgccca
     1921 ccgccaccag ccgcggcagc agcagccccg ctacccgccc gccagccccc atcgcgcgga
     1981 tgaacgcctc ggtgatggtg cgcgcggcgc gctggctggg caccagcagg gtgagcccgg
     2041 ccagccccat gggccctgcg ccataacgcg ggatcaaccc tgccaccagc gcatcggcaa
     2101 agccgcgatg ggcggcgatt gaatagaccg cgggggaatg atgcgccggg ccgccagcgc
     2161 gggcgctggc gcggccctca accatgcgtc agcagcgcct ctgtgggacc gatatggccg
     2221 ggcgcgccca cctcgcacca ttcgccggtg tgcaccaggc cgaacaggcg cccctcggcc
     2281 atcgccctgt cccacaggcg gttggtggaa aagctgcctg ctgggcaatc gcgcagcagg
     2341 cggtgcgaga cgatctggat gccggtgtag atgaagggcg ccacccgccg ctcggcccgc
     2401 cgggccacgc ggccttccgg atccagccgg aaatcgccct ggccgtgata attgtgggcg
     2461 ccggtgtggc gcaccatcag cagcagtgca tccatccgct gcgcatccca cgcgcccgac
     2521 agttcggcaa acacattgcg cgggccatcc agccagacat tgtcgctgtt gaggcagaag
     2581 aagggatcgg gcagcagccc ttgcgccagc gcccgggcca tgccgccgcc ggtttccagc
     2641 agggccgccc gctcgtcgga aatgacgact gcgggggcct tgcgctgggc cagatgcgct
     2701 tccagctgat cggccagata atgcacattg accaccgcgc gcgccacgcc tgcctcggcc
     2761 agccggtcca gcgtatggtc gatcagcgcg cggccggcca ctttcaccag cggcttgggg
     2821 cgggtggcgg tcaacgggcg catgcgcttg ccaaggccgg cggccatcac catggcggtg
     2881 tcggaaacca acgcgctcat gcggcaaggc tcccgccatg ggcggcgcgg atggcggcgg
     2941 ggatgttggc ttcaaaccag cgcgccaccg gcgccagcgc cggatgggcc aggtcccgct
     3001 ccatctgcgc ccacacgcgc ggcaccagcg ccagatagcg ctgcttgcca tcgcgctgcc
     3061 acagccgcac gaaaatgccg acgatcttgg cattgcgctg ggcgccaagg cgggcataat
     3121 cggcgaggaa atcagcgctc tccgcctccc ccgcaaggcc ggccgcggcc agatagcgcg
     3181 ccagcatcgc cgcttccacc gccggtgcca catcgcgccg ggcatcctgc aacaggctga
     3241 caagatcata ggccgggtgg cccaccagcg catcctgata atccagcagg ccctgatcgg
     3301 ccggatcgcc cgccagcagc atgatgttct cggcgtgata atcgcgcagc acggcaacgc
     3361 cgggctgctg gcgcgccatc agcggggcca gcgcctcctg ccaggccgcc tcccagccgg
     3421 gcgcatccac cgccagcccc tgcgccgggc agaaccattc cacaaacagc cgcgcctcgc
     3481 gcagataagt cgccatgtcg taaggcggga acggccccgg cgccttgccg cgcagggcca
     3541 ccagcgtgtc gatcgcggcg gcataggcgg gcatttccag cgccggcgtc tcgtccagca
     3601 catcgcgcag gcgcgcatcg ccaaagtctt ccagcagcac ccagcccgcg cccgcttcct
     3661 cggccagcac ggcgggcggg cgcaggccct gtgcggccag ccagccgccc acggccagga
     3721 acgggcgcgg atcttcctgc ggcggcggcg catgcatcag catcgcgctc tggctggcct
     3781 tgtggatgcg g
//
LOCUS       DASRYE010000043         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_108590,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000043 DASRYE010000000
VERSION     DASRYE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108590"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFF98_02520"
     CDS             <1..675
                     /locus_tag="VFF98_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU62531.1"
                     /translation="KSSAARQVAALVAAGVLVWTADRRLAPGPAFAPPPAPLAPGAPA
                     PANREGDPLEAALDSWASGYDPLLARAYGITTRLLRLAREMERQMAASAAREGMRAGD
                     VLLLDALYRVGPPHRATATELKHHFLMTLAGIAKRVDRLEALGFIARPCNPGDGRGRL
                     IALTDAGQAALARMVARDQAAPHICWPMALPDREYAALAAALARADALLGGPGAADAI
                     KERSDY"
     gene            781..1986
                     /locus_tag="VFF98_02525"
     CDS             781..1986
                     /locus_tag="VFF98_02525"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU62532.1"
                     /translation="MRSPPVAARPALQDAGPLSTSALIDSARYVSGCFGAIDPGLAAR
                     VGRLVEWLNDQPPLAAERKAEVELQLRKLLATRLRLAADRARLPGIAQERIERPVFVI
                     GFGRTGTTLLHSLLAEDPELQAPLWWHSHAPSPPPGEVPVVPARIEQAGQDLDRMLHL
                     APGLLTLHPYWDQRGLCPIEDEEIFTLDFQNAYPTLLYRVPALAMLLDAHNAGQAYGF
                     HRQFLQHLQWNQPPRRWALKGIYHQFALDALFAAYPDALCIWPHRDPLEVHPSILAIT
                     SVLYGAITDWAMDFRALGPAFVDSISQSLAATLDNPLVDDPRIVHMRFEDVVADPVAM
                     IRRAYGQWGQAVSPAHEARMRQWLANPANRPDRYGRYTYALEPFGLAEADIERAFRNY
                     RQRFITSAA"
     gene            2001..3275
                     /locus_tag="VFF98_02530"
     CDS             2001..3275
                     /locus_tag="VFF98_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62533.1"
                     /translation="MQMNEDGYFLSSHNPIANIDQRDLERLAMRLFARADVEAACARA
                     GIGWRRVTDRLMPADQMARFADHLSDYCFKCTLLAANSDANRPRVLRVYSEGAQWLGH
                     CVPGAKWGGDNPNNAYRIIPVQAGSRYELSGQRAPSPATYVTFQIVGNTTTSATLASL
                     EQLDMEIAADGSYRLTLDSTPADGRRNHLTIPPGALYLFIRDSMGDWASQAPDALRIR
                     MTSPPTAAPLDEDALAAAAIRNIQQDLFYAYYAQRLFFNGPQMMTPPEGAGSVGGLVT
                     QQGSLGHFTLAADEAVILTASEGGATYRDVVLHDLWLRSLPNRDAQISQTNAQMRADA
                     DGRFTHVIALADPGVYNWLDPCGLHDVLVLYRWQGFPDPAAPGPQLESRKVKLAELEA
                     HLPAGVARVSAAERAAQIAARQAAYDRRFHTG"
     gene            complement(3302..>4811)
                     /locus_tag="VFF98_02535"
     CDS             complement(3302..>4811)
                     /locus_tag="VFF98_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU62534.1"
                     /translation="AGGCLSFAGFAAAVRQLASALVAHGLAHGDRVAIWAPNSAQWIL
                     AAAATESIGAIMVPINTRFKGPEAAFVLAKTRARLLFTVSGFLGQDYAAMLVAACGGP
                     GDGRPAQDLPQLEQIIPLDTPALGTFSQLQAEPALLAARMAAVTPATIADILFTSGTT
                     GQPKGAMHNHGQALWMPAIWNMANDLRAGERMAIVNPFFHSFGYRSGWVSALIAGITI
                     YPLASFDAGALLALIAREKISVLMGPPTLFQGLMEHADFATTDLSSLRVGHTGAAKVP
                     VELIRAGHEVFGFELFLTSFGQTESTALVSVNYPGADAQTIATTVGRPLPGVELRIID
                     SQGQPLPPGESGELLVRGPNVMQGYFEEPEQTAAAIDAEGWLHTGDVGLIDAAGNLRI
                     LDRLKDVVIVGGFNAYPAEIEAVLRTHPAIADVCVIAWPDARMGEVCAACVVPRAGMA
                     LDLAALSAWCRERMANYKVPRHLFLVPAFPRTPLGKVQKFLLRDQVKALAGT"
BASE COUNT          741 a         1687 c         1618 g          765 t
ORIGIN      
        1 aaatcctcgg cggcgcggca ggtggcagcg ctggtggcgg ccggcgtgct ggtgtggacg
       61 gcagaccggc ggctggcgcc cggcccggcc tttgcgcccc cgccggcgcc ccttgcgccc
      121 ggggcccccg cgccggccaa ccgcgagggc gacccgctgg aggccgcgct cgacagctgg
      181 gccagcggtt atgatccgct gctggcgcgc gcctatggca tcaccacccg cctcttgcgc
      241 ctggcccgcg agatggagcg gcagatggcc gcctctgccg cgcgcgaggg gatgcgggcg
      301 ggcgatgtgc tgctgctcga tgcgctctat cgcgtggggc cgccgcatcg cgccaccgcc
      361 accgaactga agcaccactt cctgatgacg ctggccggca ttgccaagcg ggtggaccgg
      421 ctggaggcgc tgggcttcat cgcccggccc tgcaaccccg gcgatggccg cggccggctg
      481 atcgcgctga ccgatgccgg gcaggcggcg ctggcgcgga tggtggcgcg cgatcaggca
      541 gcgccgcata tctgctggcc gatggccctg cccgatcggg aatatgctgc gctggcggcc
      601 gcgctggcgc gggccgatgc cctgctgggc gggccgggcg ctgccgatgc gattaaggaa
      661 cgatcggact attaaacatc gcatctgtgt agatatgcgc gatacttgcg gaaattcttg
      721 ccagacctgg ggccctgttc gaaaatatac gaaaccggga acaccagatt gggagaagat
      781 atgcgctcgc cccctgttgc cgccaggcct gccctgcagg atgccggccc cctttccaca
      841 tccgcgctga tcgacagcgc ccgctatgtc agcggctgtt tcggcgccat cgatccgggg
      901 ctggccgcgc gggtgggccg gctggtggag tggttgaacg atcagccccc gctcgccgcc
      961 gagcgcaagg ccgaggtgga gctgcaattg cgcaagctgc tggccacgcg cctgcgcctg
     1021 gcggccgatc gcgcccgcct gccgggcatt gcccaggaac ggatcgagcg gccggtcttc
     1081 gtcatcggtt ttggccgcac gggcaccacc ctgctccatt cgctgctggc cgaggacccg
     1141 gagctgcagg cgcccctgtg gtggcacagc cacgcgccct cgccgccgcc gggcgaagtg
     1201 ccggtggtgc ctgcgcgcat cgaacaggcc gggcaggatc tcgaccggat gctgcatctg
     1261 gcgccgggcc tgctcacctt gcacccctat tgggatcagc gcggcctgtg cccgatcgag
     1321 gatgaggaga tcttcacgct cgatttccag aacgcctatc ccaccctgct gtatcgcgtg
     1381 ccggcgctgg cgatgctgct ggatgcgcac aatgccggcc aggcctatgg cttccaccgc
     1441 cagttcctgc agcacctgca atggaaccag ccacccaggc gctgggcgct gaagggcatc
     1501 taccaccagt tcgcgctgga tgcgctgttt gccgcctatc ccgatgcgct gtgcatctgg
     1561 ccgcaccgcg atccgctgga agtgcacccc tcgatcctgg cgatcacgtc ggtgctgtat
     1621 ggcgcgatca ccgattgggc gatggatttt cgcgcgctgg gcccggcctt tgtggatagc
     1681 atcagccagt cgctggccgc cacgctcgac aatccgctgg tcgatgatcc gcgcattgtc
     1741 cacatgcgct tcgaggatgt ggtggccgat ccggtggcga tgatccggcg cgcctatggc
     1801 cagtggggcc aggccgtcag ccccgcgcat gaggcgcgca tgcgccagtg gctggccaac
     1861 cccgccaacc ggcccgaccg ttatggccgc tacacctacg cgctcgaacc tttcggcctt
     1921 gccgaggcgg atatcgagcg cgcctttcgc aactatcgcc agcgcttcat caccagcgcg
     1981 gcctgaaggg agacagaccc atgcagatga acgaggatgg ctatttcctc tccagccaca
     2041 accccatcgc caatatcgac cagcgcgacc ttgagaggct ggccatgcgc ctgtttgcgc
     2101 gggccgatgt cgaggccgcc tgtgcgcgcg ccggcatcgg ctggcgccgc gtgacagacc
     2161 ggctgatgcc ggccgaccag atggcccgct ttgccgatca cctctcggac tattgcttca
     2221 aatgcaccct gctggcggcc aacagcgatg ccaaccgccc gcgcgtgctg cgggtataca
     2281 gcgagggagc gcaatggctg ggccactgtg tgcccggcgc caaatggggg ggcgacaatc
     2341 cgaacaatgc ctatcgcatc atccccgtgc aggcgggcag ccgctatgag ctgagcggcc
     2401 agcgggcgcc atcgcctgcc acctatgtca ccttccagat cgttggcaac accaccactt
     2461 cggccacgct cgcctcgctg gaacagctcg acatggagat cgccgccgat ggctcctatc
     2521 gcctcacgct cgattccacc ccggccgacg ggcggcgcaa ccatctcacc attccgcccg
     2581 gcgcgcttta tctcttcatc cgcgattcga tgggcgactg ggccagccag gcccccgatg
     2641 cgctgcgcat ccgcatgacc agcccgccca ccgccgcccc gctcgacgag gacgcgctgg
     2701 ccgccgctgc catccgcaac atccagcagg atctgttcta tgcctattac gcccagcgcc
     2761 tgttcttcaa cgggccgcag atgatgaccc cgcccgaagg cgccggctct gtcggcgggc
     2821 tggtgacgca gcagggcagc cttggccatt tcaccctggc cgccgatgag gcggtgatcc
     2881 ttaccgccag cgaaggcggc gccacctatc gcgatgtggt gctgcatgat ctgtggctgc
     2941 gcagcctgcc caaccgcgat gcccagatca gccagaccaa cgcccagatg cgcgccgatg
     3001 ccgatggccg cttcacccat gtcatcgcgc tggccgatcc gggcgtgtat aactggctgg
     3061 atccttgcgg cctgcatgat gtgctggtgc tctatcgctg gcagggcttt cccgatccgg
     3121 cagcgcccgg cccgcagctg gaaagccgca aggtgaagct ggccgagctg gaggcacatt
     3181 tgccggcggg cgtggcgcgg gtgagcgctg cggagcgcgc cgctcagatc gccgcccgcc
     3241 aggccgccta cgaccggcgc ttccacaccg gctgagggct caggcagcgc tggccggcgc
     3301 tttaggtccc ggccagcgcc ttcacctgat cgcgcagcag gaacttctgc accttgccca
     3361 gcggcgtgcg cggaaaggcg ggcaccagga acaggtggcg cggcaccttg taattggcca
     3421 tgcgctcgcg gcaccaggcg gaaagcgcgg ccagatccag cgccatccct gcgcgcggca
     3481 ccacgcaggc ggcgcaaacc tcgcccatgc gcgcatccgg ccaggcgatc acgcagacat
     3541 cggcgatcgc cgggtgggtg cgcagcaccg cctcgatctc ggccggatag gcgttgaacc
     3601 cgccaacgat caccacatcc ttcagccggt cgaggatgcg caggttgccc gccgcatcga
     3661 tcaggccgac atcgccggtg tgcagccagc cttcggcatc gatggccgcg gcggtctgct
     3721 cgggctcctc gaaatagccc tgcatcacat tggggccgcg caccagcaat tcgccgcttt
     3781 cgccgggcgg caggggctgg ccctggctgt cgatgatccg cagctccacg ccgggcagcg
     3841 ggcggcccac ggtggtggcg atggtctgcg catcggcgcc gggatagttg accgacacca
     3901 gggcggtgct ttccgtctgc ccgaagctgg tgaggaaaag ctcgaagccg aacacctcgt
     3961 ggccggcgcg gatcagttcc accggcacct tggcagcgcc cgtgtgcccc accctcagcg
     4021 aggagaggtc ggttgtggca aaatcggcat gttccatcag cccctgaaag agggtgggcg
     4081 ggcccatcag cacgctgatc ttctcgcgcg cgatcagtgc cagcagcgcg ccggcatcga
     4141 agctggccag cggatagatg gtgatcccgg cgatcagcgc cgacacccag cccgaacgat
     4201 agccgaagct gtggaagaag ggattgacga tggccatccg ctcgcccgcg cgcagatcat
     4261 tggccatgtt ccagatcgcc ggcatccaca gcgcctggcc gtgattgtgc atggcgccct
     4321 tgggctggcc ggtggtgccg ctggtgaaca ggatgtcggc gatggtggcc ggcgttacag
     4381 cggccatgcg cgcggccagc agggcgggct ctgcctggag ctggctgaaa gtgccgagcg
     4441 cgggcgtgtc gagcgggatg atctgttcga gctgcggcag atcctgggcg gggcggccat
     4501 cgcccgggcc gccgcaggcc gccaccagca tggcggcata atcctgcccg agaaaaccgc
     4561 tgacggtgaa cagcaggcgc gcgcgcgtct tggccagcac gaaggcggcc tcggggccct
     4621 tgaagcgggt gttgatcggc accatgatcg cgccgatgct ttcggtggcg gccgccgcca
     4681 ggatccattg cgcgctgttg ggcgcccaga tcgccaccct gtcgccatgg gccaggccat
     4741 gggccaccag cgcgctggcc agctgccgca cggccgcggc aaagccggca aagctgaggc
     4801 agccgcccgc c
//
LOCUS       DASRYE010000044         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_2480,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000044 DASRYE010000000
VERSION     DASRYE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1758)
                     /locus_tag="VFF98_02540"
     CDS             complement(43..1758)
                     /locus_tag="VFF98_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62535.1"
                     /translation="MAYEFGSGGQVYSYPNPLKVQNWFLAGATALSLGAGLGMLLQIR
                     ASLHTMGAGHALGALVLAFVALSVGIYLGFQLMGQLRFLFGRGRPQGLAPQLGPEEQG
                     SSPAAEHYVKEALRQQALDYAEPADPLSGLLYAVMPKLIYAPPPLRGFAERQFQAGLL
                     TISLMLGLGAAIVLGPGGGDGAGGSVSGWIALIFFLIAIPSLWRPQAGAGAAGGINLL
                     SVPQMAGLIGFSVLGPVLLSLAGTHLPRCPIEITPGWLFLLLALALVLHGLFFAAVAR
                     NALPAPSTAVSGLQESWNISTNPALISGEAMRALQEVWRDQIPNRRYTRIDPVVQLHQ
                     RSGNFRGSLVEETQPVPFLTDAPSQQPGHMSADPVLLLAINAYALAMTLWAAAASFAV
                     VSALSQPDLFTASGWPYTLFYWALALAGFDAARRLALRFDFRSQLVWLEMEGSFQSAQ
                     VALGNQFHANLQASSQVVQVEAMTFRLWAADVHSIAFGKDGRRYIYAMMGIPDYAAGL
                     AARLRGFAANQAAVVSLGNSGDVERMSRIHQLNAQMATPPAPGGQIDHGGQFPLPGGP
                     FPPAR"
     gene            complement(1881..2873)
                     /locus_tag="VFF98_02545"
     CDS             complement(1881..2873)
                     /locus_tag="VFF98_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62536.1"
                     /translation="MAGGSKVDTVYLATLVGAERAGRLLAHAGLVPAGAAQGCWPARI
                     DMVDFWRCCAQNILLTNDESHGVAAEPVPRGSLSVLITAAKEADTLAGALRRFVEAAR
                     LVRKECRLSLSRGRGVLRLTVAPARTGSLRAEIYVECFAIVAHCALRWMTGRRLQPVR
                     VRGAALLRDMGGELLDALHAPVARRGEGVSIDYALADLTAPVLPQKYSAWGEAEFANF
                     VAMLGQEEAPLLPRDHAQVLALLERGLSSQEDVAAALRISVPTLRRRLAASNLSFREI
                     SADYRTRRLKDLLATNLPLADIAEQLGLSDERSLRRFARDHLGTAPAHYREAVA"
     gene            3044..5326
                     /locus_tag="VFF98_02550"
     CDS             3044..5326
                     /locus_tag="VFF98_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62537.1"
                     /translation="MAGFSTSAWLGCVALAALAQGQAAHAGESTAPPPPAAIIDDVPI
                     VVTASRRSESLQNVGGQVTALGGAALEQMHASTFNDFATSVPGLSFQANSPTNNLIAI
                     RGVASSTAELGGAVSIYLDDVPIGASTQFGLGSQSFNFNLFDMDRVEVLNGPQGTLYG
                     ANALGGAIKYITAKPQLGKLAAKLELDGSATDHAGTNGAVRAMVNVPLGDMLALRVDG
                     MVQHDSGWGNDPARGLKRLGSSDTAGGRVALLFKPTDELEVRLSAFGQRSKANGLNIG
                     FYNIGNSTPVAGPYSQQFTLDQPSYNSVSLLSGEVSYDFGFAKAVSVTSYQKNHGFYN
                     TDDSVFYGVLVPIYEYLYNSYVPASPYNLYVDTRTNKFSQEFRLQSPSNHHIEWVAGA
                     YYTHEFTGEQVNLLYTASGTGNMPAPYSSYPFYGWLPSHYTEVAGFGDVTAYIGKIFD
                     ITLGIRYSHQDQLYESNIWWLGFGPTLVGSTYVYGTPAYHAGTSSQGVTTYLINPRLH
                     ITRDVMVYGRVSSGFRPGGPNFFLGSSTLPATFQPDKLWNYEIGEKGSFFDHHLTFDI
                     AAYDIEWSSIQTTQNINGINQLVNAGNARVQGVETSFVLKAAPGFTVNGSAAYTDAHL
                     TTTAPVLGVYYTGARLPLSPHFNFALGATYRFTLAGLPTTAQLTDIWTGSRTSGYYGS
                     ATNVYYRMPAYNTVNGSIGFTLPAGFEVDAYVKNLFNSHGQLSANTLNNAVLASAPVP
                     VTLAQPLTGGLTLKYAWGGR"
     gene            5330..>6243
                     /locus_tag="VFF98_02555"
     CDS             5330..>6243
                     /locus_tag="VFF98_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HZU62538.1"
                     /translation="MATPALYDSLFATVETSLGKVRGLVNAGVRIFRGIPYGAPTGGA
                     TRFQPPLPAAPWAGVRDCFAPGHVAPQIPTPITHTYAQLIHFDMAPADGGIGEGCLTL
                     NVFAPTADDGRLRPVMVCLHGGGFAIGSGNAAMYDGTQLAFEQDVVVVAINHRLACFG
                     FLGLAALGADERFEAAGVVGLLDIVLALEWVRANARGFGGDPASVTLFGQSGGGWKVS
                     SLLAMPAARGLFHRAVVQSGSWLAFQPAEQAGALAAALLAQLGLGPTDWPLLLEADMG
                     AILAAQAGVGALAYQPVLHPVHLPHHPAS"
BASE COUNT         1062 a         2184 c         2010 g          987 t
ORIGIN      
        1 cggcgcgctg gtggatgccc ccgcgcctgc ccgcagcgca gcctagcgcg ccggcggaaa
       61 cggcccgccc ggcagcggga actggccgcc atgatcgatc tgcccgccgg gcgcgggcgg
      121 cgttgccatc tgcgcgttca gctggtggat gcggctcatc cgctccacat cgccgctgtt
      181 gcccagcgac accaccgccg cctgattggc cgcaaagccg cgcagccgcg ccgccagccc
      241 ggccgcataa tcggggatgc ccatcatcgc gtagatatag cgccggccat ccttgccaaa
      301 ggcgatggaa tgaacgtctg ccgcccacag gcggaaggtc atcgcttcca cctgcaccac
      361 ctggctgctg gcctggaggt tggcgtggaa ctggttgccc agcgccactt gcgcgctctg
      421 gaagctgccc tccatctcca gccagaccaa ctgcgagcgg aaatcgaagc gcagcgccag
      481 ccgccgcgcg gcatcgaaac cggccagcgc cagcgcccag tagaacagcg tgtagggcca
      541 gcccgaggcc gtgaacagat ccggctggct gagcgccgag acaaccgcga aactggccgc
      601 cgccgcccac agcgtcatcg ccagcgcata ggcgttgatc gccagcagca gcacggggtc
      661 ggcgctcata tggccgggct gctggctggg ggcgtcggtc aggaagggca ccggctgggt
      721 ttcttccacc aggctgccac ggaaattacc ggaacgctgg tgcagctgca ccaccggatc
      781 gatgcgggtg tagcgccggt tggggatctg gtcgcgccac acttcctgca gggcgcgcat
      841 cgcctcgccg gaaatcagcg ccggattggt ggagatgttc cagctttcct gcaggcccga
      901 caccgccgtg gaaggcgccg gcagggcatt gcgcgccact gcggcaaaga acagcccatg
      961 cagcaccagc gccagcgcca gcagcaggaa cagccagccc ggggtgatct cgatcgggca
     1021 gcgcggcaga tgcgtgccgg ccagcgacag cagcaccggc cccagcaccg aaaagccgat
     1081 cagcccggcc atctgcggca cggacagcag gttgatgccg cccgcagcgc ccgcgccggc
     1141 ctgcggccgc cacaggctgg gaatggcgat gaggaagaag atcagcgcga tccacccgct
     1201 gacagagccg cccgcgccat cgccgccgcc cggccccagc acgatggccg cgcccagccc
     1261 cagcatcagg ctgatggtca acaggccggc ctggaactgc ctttcggcaa agccgcgcaa
     1321 gggcggcggg gcatagatca gcttgggcat caccgcatag agcaggcccg aaagcggatc
     1381 ggcgggctcg gcataatcga gcgcctgctg gcgcagcgct tccttcacat aatgttcggc
     1441 ggccgggctg ctgccctgct cttccgggcc cagctgcggc gccagcccct gcgggcggcc
     1501 gcggccaaac aggaagcgca attgccccat cagctgaaag ccgaggtaaa tgcccacaga
     1561 cagcgccaca aaggccagca ccagcgcgcc cagcgcatgg cccgcgccca tcgtgtggag
     1621 gctggcgcgg atctgcagca gcatgccaag gcccgccccc agcgacagcg cggtggcgcc
     1681 ggccaggaac cagttctgca ccttcagcgg attgggatag ctgtagacct gcccaccgct
     1741 gccgaattca tacgccatgc ccgagatacc tcacctgcgc ggatcgcccg ccgcccggga
     1801 taaaccccga aagaacaagg gacgattcgc tgccgcgcgc ctgtttagtg gggggccggc
     1861 gggctgtcga tagaaggctc tcaggccacc gcctcgcggt agtgggcggg cgccgtgccc
     1921 agatgatcac gcgcgaagcg gcgcagcgaa cgctcgtccg acaggcccag ctgctcggca
     1981 atgtcggcca gcggcaggtt ggtggccagc aggtccttca ggcggcgggt gcggtaatcg
     2041 gcggaaatct cgcggaagga cagattgctg gcagccagcc gccggcgcag cgtgggcacg
     2101 ctgatgcgca gggccgcggc cacgtcctcc tgcgaggaaa gcccgcgctc cagcagcgcc
     2161 agcacctggg catggtcgcg cggcagcagc ggcgcttcct cctgccccag catggcgacg
     2221 aaattggcga attccgcctc gccccaggca ctgtatttct gcggcagcac cggcgctgtc
     2281 aggtctgcca gcgcatagtc gatgctcacc ccctcgcccc ggcgcgccac aggggcgtgc
     2341 agcgcatcca gcaattcgcc gcccatgtcg cgcagcagcg ccgcgccgcg cacgcgcacc
     2401 ggctgcaggc gccgcccggt catccagcgc agcgcgcaat gggccacgat ggcaaagcat
     2461 tccacataga tctcggcgcg caggctgccg gtgcgggccg gcgccactgt cagccgcagc
     2521 acgccgcgcc cgcggctgag cgagaggcgg cattccttgc gcaccagcct tgccgcttcc
     2581 acaaagcggc gcagggcgcc ggccagcgtg tcggcctcct tggcagcggt gatcagcacc
     2641 gagaggctgc cgcgcggcac cggctctgcc gccacgccat ggctttcgtc attcgtcagc
     2701 aggatgtttt gcgcgcagca ccgccagaaa tcgaccatgt cgatccgcgc cggccagcag
     2761 ccttgcgccg cgcccgccgg caccagcccg gcatgggcca gcagccggcc ggccctttcg
     2821 gcgcccacca gcgtggccag atagaccgtg tcgaccttgc tgccgcccgc catcatcctc
     2881 tcctggcata cttgtgtttt acagggagat tatgcctgtt gacgcggttg tcaacgagcg
     2941 aaaacagccg ctgcactttg cgcgcttgtg tcctcttcgc cgggccatgc cagcgttatt
     3001 ctggcggcat aacaaaaact gccgacaatc gagggaggat ctcatggcag gcttcagcac
     3061 atccgcatgg cttggctgcg tggcgctggc ggcgctggcg caggggcagg ccgcccacgc
     3121 cggcgaaagc accgcaccgc cgccgcccgc tgccatcatc gacgatgtgc cgatcgtcgt
     3181 caccgccagc cggcgcagcg aaagcctgca gaatgtgggc ggccaggtga ccgcgctggg
     3241 cggcgccgcg ctggaacaga tgcacgcctc caccttcaac gatttcgcca cctcggtgcc
     3301 cggcctcagc ttccaggcca acagcccgac gaacaacctc atcgccatcc gcggcgtggc
     3361 ctcttccacg gcggaactgg gcggggcggt gtcgatctat ctcgacgacg tgccgatcgg
     3421 cgccagcacc cagtttggcc tgggctcgca aagcttcaac ttcaacctct tcgacatgga
     3481 ccgggtggaa gtgctgaacg ggccgcaggg cacgctttat ggcgccaatg cgctgggcgg
     3541 ggcgatcaaa tacatcaccg ccaagcccca gctgggcaag ctggccgcca agctcgagct
     3601 ggatggatcg gccaccgatc atgccggcac caatggcgcg gtgcgggcaa tggtgaatgt
     3661 gccgctgggc gatatgctgg cgctgcgcgt ggatggcatg gtgcagcacg acagcggctg
     3721 gggcaacgat ccggcccgcg ggctgaagcg gctgggcagt tccgataccg ccggcggccg
     3781 cgtggcgctg ctgttcaagc cgaccgacga gctggaagtg cgcctttccg cctttggcca
     3841 gcgcagcaag gccaatggcc tcaacatcgg cttctacaac attggcaaca gcacgccggt
     3901 ggctggcccc tacagccagc aattcacgct tgatcagccg tcttacaatt cggtcagcct
     3961 gctgtcgggc gaggtgagct acgacttcgg ctttgccaag gcggtgtcgg tcacatccta
     4021 tcagaagaac cacggcttct ataataccga cgacagcgtg ttctatggcg tgctggtgcc
     4081 gatctacgaa tatctttaca acagctatgt gcccgccagc ccctataacc tctatgtcga
     4141 cacgcggacc aacaagtttt cccaggaatt ccgcctgcaa tcgcccagca accaccatat
     4201 cgaatgggtg gccggcgcct attacacgca tgagttcacc ggcgagcagg tcaacctgct
     4261 ctacaccgcc agcggcaccg gcaacatgcc cgcaccctat tcctcctatc ccttctatgg
     4321 ctggctgcct agccactaca ccgaagtggc cggctttggc gatgtgaccg cctatatcgg
     4381 caagatcttc gatatcaccc tgggcatccg ctattcgcac caggatcagc tgtatgaatc
     4441 gaacatctgg tggctgggct ttggccccac gctggtgggc agcacctatg tctatggcac
     4501 gccggcctat catgccggca cctccagcca gggcgtcacg acctatctca tcaacccgcg
     4561 cctgcacatc acccgggatg tgatggtcta tggccgggtg tcctcaggct ttcggccggg
     4621 cgggcccaat ttcttcctgg gcagcagcac gctgcccgcc accttccagc ccgacaagct
     4681 gtggaactat gaaatcggcg agaagggcag cttcttcgac caccacctga ccttcgacat
     4741 cgccgcctac gacatcgaat ggagcagcat ccagaccacg cagaacatca atggcatcaa
     4801 ccagctggtg aatgccggca atgcccgcgt ccagggcgtg gaaaccagct tcgtgctgaa
     4861 ggcagcgccc ggcttcacgg tcaatggctc ggccgcctac accgatgccc atctcaccac
     4921 cacagcgccg gtgctggggg tctattacac aggcgcgcgc ctgccgctct cgccgcattt
     4981 caacttcgcg ctgggggcca cctatcgctt caccctggcg ggcctgccca ccaccgccca
     5041 gctgaccgac atctggaccg gcagccgcac ctccggctat tacggcagcg ccaccaacgt
     5101 ctattatcgc atgcccgcct acaacacggt gaacggcagc atcggcttca ccctgccggc
     5161 cggcttcgaa gtggacgcct atgtgaagaa cctgttcaac agccacggtc agctctccgc
     5221 caacacgctg aacaatgcgg tgctggccag tgcgccggtg ccggtcacgc tggcccagcc
     5281 gctcaccggc gggctgacgc tgaaatacgc ctggggcggg cgctgaccca tggccacgcc
     5341 tgccctctac gacagcctgt tcgccacggt ggaaaccagc cttggcaagg tgcgcgggct
     5401 ggtgaatgcc ggcgtgcgga tctttcgcgg cattccctat ggggcgccca ccggcggggc
     5461 gacgcggttt cagccgccgc tgccggccgc gccctgggca ggcgtgcgcg attgctttgc
     5521 ccccggccat gtggcgccgc agatccccac gcccatcacc cacacctatg cccagctcat
     5581 ccatttcgac atggcgccgg ccgatggcgg cattggcgag gggtgcctca cgctcaatgt
     5641 ctttgcgcca acggcggatg atggccggtt gcggccggtg atggtgtgcc tgcatggcgg
     5701 gggctttgcc atcggctctg gcaatgcggc gatgtatgac ggcacccagc tggccttcga
     5761 gcaggatgtg gtggtggtgg cgatcaacca tcggctggcc tgcttcggct ttctggggct
     5821 ggcggcgctg ggggcggatg agcgctttga ggcggcgggc gttgtggggc ttctcgatat
     5881 cgtgctggcg ctggaatggg tgcgcgcgaa tgcgcggggc tttgggggcg atccggcctc
     5941 ggtcaccctg ttcggccagt ccggtggggg gtggaaagtc tcctcgttgc tggcgatgcc
     6001 ggcagcgagg gggcttttcc atcgcgccgt ggtgcaaagc ggatcctggc tggccttcca
     6061 gccggccgag caggcaggcg ccctggccgc cgccctgctg gcccagctgg gccttggccc
     6121 gacagactgg cccctgctgc tggaggccga catgggcgcg atcctggcgg cgcaggcagg
     6181 cgtgggggcg ctggcctatc agccggtgct gcatcctgtg cacctgcccc atcacccggc
     6241 ctc
//
LOCUS       DASRYE010000045        35323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_89803,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000045 DASRYE010000000
VERSION     DASRYE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 35323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35323
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89803"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFF98_02560"
     CDS             complement(<1..863)
                     /locus_tag="VFF98_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62539.1"
                     /translation="MPQPSGAGGLRAWLAVAGVPLLRHQLALARALGVQRLLCLHEAD
                     GPPLGDVEGEAALFGLRVQRAANAHALLAQVSAADEVIVLADGLLARAEVIVPLLEAG
                     PAVLVQPVETGLATGFERIDLNHAAAGAMLLPGRLVSRLADLPADVHVPSALLRVALQ
                     AGVAQKPLPADAAHGGGWALLRDEAAAEQAEAAFLQLHAPDVARQPPGTRLAAWLARR
                     FAPALLASGNNGLSMAVAGVLALALGLVFGWLVFPALALLGLGLAAVLLGAAGVLGRM
                     VAAGLNPGRPL"
     gene            complement(983..1288)
                     /locus_tag="VFF98_02565"
     CDS             complement(983..1288)
                     /locus_tag="VFF98_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZU62540.1"
                     /translation="MVCETGSLDAMIAAAAGDDEALFRELRASFLDSLARQIDLLGRA
                     RCDGNWQVAALRLRGLGASFQAWPLIELAEEALGGAPGEPGVLRRLRALHGQLAGTE"
     gene            1379..3730
                     /locus_tag="VFF98_02570"
     CDS             1379..3730
                     /locus_tag="VFF98_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62541.1"
                     /translation="MAAQEQTETALVAPLIEEAPAPTPEAPALVAEPESPADLPELEA
                     PQWPVRLAQGLALAALLGWTGLALATHLAALADAAQWAAFVGLWAPPALLLVLLVLAV
                     GRLGGGAGPRLLVATRALDAEAARLEQRLSAMNRELSLAREFLASQSRELETLGRLAV
                     ERVNEHGARLAGYVEGSATHVQVLGTVSEAAAHNMERVRQHLPVLTQAARDVANQIGN
                     AGRLAENHVQELVNGFTKLNAFGQAGQSHVQAFQEQTEATLAGLDARVAQLASQLEGR
                     IAAMRGLMADAHGAIAAREEAAGAVLGERIAALQAEIAAARATLDGEEAQSLTSLRAR
                     LSALRDESQTIGRALRDQEHTALGQWRAEAGEMAADIARRQRAHLADAETLAATLDTT
                     RQRLTDIEARLTAIGASDAALDSRLVARLGQVDSRLADTEAALARLGEAGAALVARIE
                     AGAEASQTALPAALAQAAEDLAAYERRVFTLRDAMRDAGERGAALSQSVQASREVLMS
                     AAGGLSGLHDGLAARVQEHAASLGLLRQSLAGLDSEAARIAATSGEALEGALGRLSGG
                     VLDAVAQIELRGARAVNELAERLRSESAAAIDSATEDRARAISAGIAEAARHAATLGS
                     ETAERLRGQLAAVEDLLGHLEQRLTEAREAAEEKVDNDFSRRAALITETLNSAAIDIA
                     KALDADIADTAWAAYLRGDRGIFTRRAVRLLDTAEARAVLLSYERDRAFAEHVNRFIH
                     DFEGMLRQLLSTRDGHALSVTLLSSDMGKLYVALAQAIERLRS"
     gene            complement(3739..4029)
                     /locus_tag="VFF98_02575"
     CDS             complement(3739..4029)
                     /locus_tag="VFF98_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HZU62542.1"
                     /translation="MKWSSMLAVYLLFWVISALFVLPFGVRTHAEVGEPLVPGQADSA
                     PANFRPGRVALRATAIAAVAFALFWLNRQHNWLSVEDISFLHPPASLGSSEG"
     gene            complement(4053..5651)
                     /locus_tag="VFF98_02580"
     CDS             complement(4053..5651)
                     /locus_tag="VFF98_02580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZU62543.1"
                     /translation="MNVNLYGCEGQWLMVDLGMTFSGNEYPGVELVFADLDFIEERRE
                     ALAGIVLTHAHEDHIGAVPYFAADLGVPLYATPFTARLVAAKLEEAGLAGEVELIVID
                     HGDPFRVGPFSIRYVPLAHSIAEGNAVVIDTPFGRIFHTGDWKLDDEPLVGRPASPEE
                     LTRIGDEGVLALVCDSTNVFNPNPSGSEGDVGRAMLEEVRRHKGKRVLVTTFASNVAR
                     LETLGRVARETGRRLCVSGRSLDRIIDAAKASGYLKQLPDLVDVDTAMELPRGEVLIV
                     ATGGQGEPRAALARIAEGNHPISLEAGDVVLFSSRQIPGNEIPIGRIQNQLAAQGITV
                     VTDRQSHIHVSGHPGRPELQALYGWIRPEILVPVHGEERHMREQGRLGKASGIARIVN
                     QKNGDLVRLAPGRPGKFAEVRAGRLVLDGDIIAPADGEAVLMRRRMAHNGVVVVGLAG
                     LSGHGGKHKSHLRIDVLGLGLPLVEDYENFVAEAREDIARALDKKPARRDVEELKEAA
                     RLAARRATQRWCGKRPQVRVMLLD"
     gene            complement(5755..6534)
                     /locus_tag="VFF98_02585"
     CDS             complement(5755..6534)
                     /locus_tag="VFF98_02585"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZU62544.1"
                     /translation="MLLAIDVGNTNVVFALFDGETIKARWRIATDPRRTADEYAVWLM
                     QLLAIEGFDRGAVAQIIISTVVPRALHNLQVLGSKYFDVEPLVAGQGKAGYAIDIDVD
                     DPKSLGADRAVNALGAHARYAGDLIVVDFGTATTIDAIDFNGAYKGGIIAPGLNLSLD
                     ALVGNTAKLPRIAIARPASPSVIGTNTEDQMLIGIYWGYVAMIEGLIARMRRQIGRPA
                     TVVATGGLAVLFNAETGLFDAVDPDLTLNGLALLATRAAAR"
     gene            complement(6574..7284)
                     /locus_tag="VFF98_02590"
     CDS             complement(6574..7284)
                     /locus_tag="VFF98_02590"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZU62545.1"
                     /translation="MISFFNEIDSTNAEWARRLAAGAPSVEGEWLVARRQTAGRGRLG
                     RAWQDGAGNFMGSTLVRPGPGDPPPASLALLTGLAVAEAVAGHVPPPLAPLLKWPNDV
                     LVNGAKLAGVLLEAQGDAVIVGVGVNLASAPLLEGRETVALADFAPPPALDHFAGQMA
                     AAFAAQLASWRAHGLAALVARWQQAALPQGTPLGVTGADGQRIEGRFAGLAEDGAMRL
                     RLADGAIRAIHAGDVALL"
     gene            complement(7292..8749)
                     /gene="nuoN"
                     /locus_tag="VFF98_02595"
     CDS             complement(7292..8749)
                     /gene="nuoN"
                     /locus_tag="VFF98_02595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZU62546.1"
                     /translation="MSWIVSLNLVAAEEILSAAGLVLMLVAAWAGDKSARAISVAAVA
                     ALVAASAFVLQGLGATGAQTSAFYGQYADDGFAAYAKLLIYLAAAVAVIVAPRFFAPR
                     DEMRAEYPVLVLFAALGMGLMVSATDLLTLYIGLELNSLAAYVLAAMLRRDERSAEAG
                     LKYFVLGALASGILLFGMSLTYGFSGSTNFVAIGKALHGQLATGALFGVVFMLVGLAF
                     KISAVPFHMWTPDVYEGSPTPVTTFFATAPKVAALCLLMRVTLVAFGAEVVAWQQIVI
                     ALSLLSIVVGALGAIGQTNLKRLLAYSSINNVGFMLIGLAAATPAGVSAVLVYLFIYV
                     FTSFAGFAAVLLLRDREGEQIESLADIAGLSRTRPALAAVLAMVMFSLAGIPPLFGFW
                     GKFVVFQAAVAAGMVPLAAIGIAASVIGAFYYLKVVKVMYFDDPADKVAPASDVAHNA
                     ILLVSAVVISPVGYLMTNWLGGVAQQAAAALFHTA"
     gene            complement(8749..10290)
                     /locus_tag="VFF98_02600"
     CDS             complement(8749..10290)
                     /locus_tag="VFF98_02600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZU62547.1"
                     /translation="MIGFPILSLMLAVPLVASLVSLALSAQGARWLALGATLVDLVLG
                     VVLWQHYQIGGPQWQFTERLPLFSGFQWALGIDGIALTLIALTVLLMPICIGASWHAI
                     EKRVPEYMAAFLLMETLMIGVFAAQDLYLFYIMFEAGLIPMYLVIGIWGGADRIYASY
                     KFFLYTLLGSVLMLIAMLWMVHEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMP
                     MWPVHTWLPDAHVQAPTAGSVILAGVLLKMGGYGFIRFSLPMFPEASAYFAPLVWGLS
                     MVAVVYTSLVALVQQDMKKLIAYSSVAHMGIVTIGLFSFNRQGLEGSMVVMLAHGLVA
                     GALFLCVGVIYDRLHTREISRYGGLAINMPKYALFFMFFTMASIGLPATANFVGEFLS
                     LAGIYQVSTWAALICTTGIILGAAYMLYLYARVVFGAQKNADAAAMPDLDLREWALLA
                     PLVAATLWMGIYPESFIAPMRADIAALDARLASARPASDAAPTAGTPAPKTEAAANTV
                     EGSAR"
     gene            complement(10319..12385)
                     /gene="nuoL"
                     /locus_tag="VFF98_02605"
     CDS             complement(10319..12385)
                     /gene="nuoL"
                     /locus_tag="VFF98_02605"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZU62548.1"
                     /translation="MHPILFIVFLPLLAAVIAGLGNKAIGKVPAKLVTTGALFAACAL
                     SWSIFCQFLGGHAEVSVTPVLQWVHSADLVFNWELRVDALTAVMLVVITTVSALVHLY
                     SWGYMSEEPDQPRFFAYLSLFTFAMLMLVTANNLVQMFFGWEGVGLASYLLIGFWFQK
                     PSACAAAIKAFVVNRVGDLGFMLGIFGIFLVFHTVDIETILRHAPDYAGSTIGFLGYR
                     VDTLTLLCLLLFVGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRL
                     SPLFQQSETALHVITFIGAATCFFAATVGTTQWDIKRVIAYSTCSQLGYMFFAAGVGA
                     YGASMFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRYYGGLWKKIPLTFVAMMAGTLA
                     ITGVGLAEVGGFAGFYSKDAIIEAAFARGTTLGQFASITGIVAALLTSFYSWRLVFLT
                     FFGKPRWNQSEHIQHAVHDAHGHDDHAHHGHDHDHAHGHHGHEDDGTAGYHPHESPLN
                     MLIPLIVLTLGAVFAGALFNRYFVGIETAGDFWKASTLFFDKELFEAAEHIPAAVKWA
                     PFEVMVAGLVGAWVCYVQVPSIPAAFVKVFKPLHAFFYNKWYFDELYNFLFVRPAFVL
                     GRAFWKLGDIGIIDRFGPNGAAAVVAKGVAGARRLQTGYLTSYALTMLLGLVAAVSWV
                     MWQSVR"
     gene            complement(12391..12696)
                     /gene="nuoK"
                     /locus_tag="VFF98_02610"
     CDS             complement(12391..12696)
                     /gene="nuoK"
                     /locus_tag="VFF98_02610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZU62549.1"
                     /translation="MIGIEHYVAVSSILFVLGVLGIFLNRKNIIIILMAIELILLAVN
                     INLVAFSAFLHDLVGQVFAMFVLTVAAGEAAVGLAILVIYFRSRGSIAVDDVNRMKG"
     gene            complement(12708..13325)
                     /locus_tag="VFF98_02615"
     CDS             complement(12708..13325)
                     /locus_tag="VFF98_02615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZU62550.1"
                     /translation="MIETLAFYLFATLVVASGTFTIFAKNPVHSVLWLILAFFNAAGL
                     MVLAGAEFIAMLLVIVYVGAVAVLFLFVVMMLDIDFATLRAGFMRNMPLGLAIAVVLL
                     AELGMGLGAWHAGGLQLGVADPATAPQAGLSNIQSIGALLYGRYLFLFEGSGIILLVA
                     MIGAIVLTHRQRTGVKTQSIWKQVSRRPADATVNTRPAVGEGVQI"
     gene            complement(13336..13821)
                     /gene="nuoI"
                     /locus_tag="VFF98_02620"
     CDS             complement(13336..13821)
                     /gene="nuoI"
                     /locus_tag="VFF98_02620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZU62551.1"
                     /translation="MSVGQLVKSFTLWEFVKAHALTLKYFFKEKATVNYPFEKNPLSP
                     RFRGEHALRRYPNGEERCIACKLCEAVCPAQAITIEAHPREDGSRRTTRYDIDMTKCI
                     FCGFCQEACPVDAIVEGPNFEYATETREELLYDKAKLLANGDKWERAIAANLEADAPY
                     R"
     gene            complement(13818..14861)
                     /gene="nuoH"
                     /locus_tag="VFF98_02625"
     CDS             complement(13818..14861)
                     /gene="nuoH"
                     /locus_tag="VFF98_02625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZU62552.1"
                     /translation="MQLLEMAGLPHGWAWFLATVIGILAIALPVMLSVALVIYFDRKI
                     WAAMALRRGPNVVGPVGILQSFADGLKVFLQETIVPSAANKGLFLIGPIVTFTVALLA
                     WAVIPFNSGAILANINVGVLYVLAISSLGVYGTIMAGWASNSKYPFFSAMRASAQMIS
                     YEVSIGFIVICVVLWAGSFNMNSIVIAQKGHGLGFINAFACNIFLFPMWVMFFISSLA
                     ETQRAPFDLTEAESELVAGYQTEYSSMSFALFWLGEYGNVLLMCALNATLFWGGYLPP
                     VEWAPLYLIPGWIWLIAKMGFGFFVFSWVKATVPRYRYDQLMRLGWKVFLPVSLLFVF
                     LVSGYLMATGHFA"
     gene            complement(14882..16900)
                     /gene="nuoG"
                     /locus_tag="VFF98_02630"
     CDS             complement(14882..16900)
                     /gene="nuoG"
                     /locus_tag="VFF98_02630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZU62553.1"
                     /translation="MPKVKVDGVEIEVPAGATVLQACELAGKEIPRFCYHERLSIAGN
                     CRMCLVEVKPGPPKPQASCALPASEGQEIRTDSEMVKKAREGVMEFLLINHPLDCPIC
                     DQGGECDLQDQSVAYGRGASRYHEHKRAVTEKYMGPLIRTVMTRCIQCTRCVRFSEEI
                     AGVDEIGALYRGENMQISTYLEKAAKHELSANVIDLCPVGALTNRPYSFEARPWELKK
                     TLGIDVSDAIGANIRIDARGREVLRILPRVNDDVNEEWLSDKGRFLVDGLTKRRLDRP
                     YIRLDGKLVPATWSEALAAVAKIAPGSSVAAVAGDLVDAETLFAAKQLLAALGSTLLE
                     GRQTGLAYDCSNLAAVNFNTTLAGIENADAVLVVGSHVRWEAPLLNTRLRKAVKKGAK
                     VFIIGPEWDPTYKAEFLGADAAILNQLPAHVWAALQEAKRPAIIVGGAGLAKGALEAA
                     LSVSDQLGLVRELEDGSQWNGFNVLHMAASRMASLMLGYAQKGGIADIAAAKPKMLLA
                     LGADEVDFAKFADSMIVYVGHHGDKAAHVADIILPGSAFTEKAGIYVNTEGRVQYADK
                     AVFAPGDAREDWAIIRALADALGVKVGFDSFDALRAALFAFNPAFGQEGLADYGALPK
                     GVTGAAAGVIAYPIADFYLTNPIARASATLQRCSAELLHGETQKEAAE"
     gene            complement(16900..18195)
                     /gene="nuoF"
                     /locus_tag="VFF98_02635"
     CDS             complement(16900..18195)
                     /gene="nuoF"
                     /locus_tag="VFF98_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZU62554.1"
                     /translation="MALADKDRIFTNLYGHQPWNLDAARKRGDWDNTKALLEKGRDAI
                     IQEMKDSGLRGRGGAGFPTGMKWSFMPKESRDGRPSFLVINADESEPGSCKDREIIRH
                     DPHKLIEGALVAGFAMGARAAYIYIRGEYIREAETLFAAVAEAYEAGLVGKNASGSGY
                     DFDVFVHRGAGAYICGEETAMIESIEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIA
                     VAPTILRRGAGWFSSFGRENNRGTKLFQISGHVDRPCVVEEEMSIPFRELIEKHCGGI
                     RGGWDNLLAVIPGGSSVPLVPAAEMIDAPMDFDGLRALGSGLGTAAVIVMDKSTDIVR
                     AISRLSYFYKHESCGQCTPCREGTGWMWRVMERLRVGDASIEEIDMLWNVTKQVEGHT
                     ICALGDAAAWPIQGLIRHFRPEIERRIAQRNGGLQEAAE"
     gene            complement(18199..18867)
                     /gene="nuoE"
                     /locus_tag="VFF98_02640"
     CDS             complement(18199..18867)
                     /gene="nuoE"
                     /locus_tag="VFF98_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZU62555.1"
                     /translation="MADRHLEPDTPELRARWGAFAWTAQNAEKAKEVVARYPEGRQRS
                     AVMPLLDLAQRQVGAETNTQGWLPIPVMEYVATYLDMPIIRVVEVATFYTMYNLVPVG
                     RFHVQVCGTTPCMLRGSDDLIAACHGRGMKAGHVSEDGLWTFTEVECMGNCASAPMVQ
                     INDDNYEDLTVERLDKVLDALARGETPKAGTQEPGRHTVEPAGALSNLTAMVSGNHDY
                     RGEW"
     gene            complement(18867..19199)
                     /locus_tag="VFF98_02645"
     CDS             complement(18867..19199)
                     /locus_tag="VFF98_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62556.1"
                     /translation="MSHADTARAMIAAYNAQDADTYVSFMTEDACEAAYRGAVVREGR
                     EGTRAGLVRMFAEFPENRAEIIDVKALGNYVVFHERVWRTAASEPFDVLAVYTFEGDK
                     CARVEFIR"
     gene            complement(19214..20428)
                     /locus_tag="VFF98_02650"
     CDS             complement(19214..20428)
                     /locus_tag="VFF98_02650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZU62557.1"
                     /translation="MSGLSLEQSPTTGDEIITNYTINFGPQHPAAHGVLRMVTELDGE
                     IVERIDPHVGLLHRGTEKLIEHKTFLQALPYFDRLDYCSPLSMEHSYVLAVEKLLNVE
                     VPLRAQYLRVLFAELTRISNHMMNIGSAVMDVGAMTPMLWFFEIREDLLNFFERASGA
                     RMHSAYYRPGGVHQDVPLKLLVDIGEWLEKRFPELYEDACSLFVDNRIFKQRNVDIGI
                     VTKEDAVRWGFSGPMLRGSGIAWDIRKSQPYDVYERMDFDVPVGTSGDCYDRMMVRVE
                     EVRQSVRIVKQCLAEMPDGPIASTDRKVSPPKRGEMKQSMESLIHHFKLYTEGFHVPA
                     GEVYVATESPKGEFGVYLVSDGGNKPYRCKIRPTAFSHLQAMDYLTKGHMLPDITAIL
                     GAMDIVFGECDR"
     gene            complement(20443..21054)
                     /locus_tag="VFF98_02655"
                     /pseudo
     CDS             complement(20443..21054)
                     /locus_tag="VFF98_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445944.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            complement(21068..21619)
                     /locus_tag="VFF98_02660"
     CDS             complement(21068..21619)
                     /locus_tag="VFF98_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZU62558.1"
                     /translation="MSTQLSPAAAAQAGQLVAPDQAFFNDLNAEVSDKGFLVTNVEDL
                     FHWARTGSLWWMTFGLACCAVEMIHANMPRYDMERFGAAPRASPRQSDVMIVAGTLCN
                     KMAPALRRVYDQMSNPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPT
                     AEALLYGVMQLQRKIRRVGTIER"
     gene            complement(21616..21990)
                     /locus_tag="VFF98_02665"
     CDS             complement(21616..21990)
                     /locus_tag="VFF98_02665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZU62559.1"
                     /translation="MVDLTQYLPILIFLVVALGLSSAFVFLPMAAARLTGSHQPDADK
                     LSEYECGFPAFEDPRSQFDVRFYLVAILFIVFDLEAAFLFPWAVSLKETGWAGWITMM
                     VFLGELVLGLIYAWKKGALDWE"
     gene            22246..23439
                     /locus_tag="VFF98_02670"
     CDS             22246..23439
                     /locus_tag="VFF98_02670"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZU62560.1"
                     /translation="MANISAQDPVVILSYARTPMGGLQGALADASATDLGATAVKAAV
                     DRAGVSPDEIERIYMGCVLPAGLGQAPARQAALKAGLPKSVQATTVNKVCGSGMQTVI
                     MGAEALAAGSIDLVIAGGMESMTNAPYLLKKHRSGARAGHDTAYDHMFLDGLEDAYEG
                     AAMGAFAQITADDYQLTREAQDAYSIESLARAKAAVAEGRFAEEIAPVTLKTRAGEVV
                     VDTDEAPGKGRPDKIPTLKPAFAKDGTITAASSSSISDGAAALVLTRKSIAEAKGLAP
                     VATLISVGAHAQEPRLFTTAPVGAIQSVLDKAGWTVGDVDLFEVNEAFACVAMFAMHD
                     LGIAHDKINVNGGATALGHPIGASGARIITTLIAALKARGLKKGVAALCIGGGEGTAI
                     AVELA"
     gene            complement(23491..23928)
                     /locus_tag="VFF98_02675"
     CDS             complement(23491..23928)
                     /locus_tag="VFF98_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZU62561.1"
                     /translation="MLAPAPPAHATAPSNPPYWASLRVDEVNMRVGPGEDYRINWVYH
                     RAHLPVKVLRVMEGWRLIEDPDGARGWVLAKFLTKERGAYVTASPSADIHAAAAAGSA
                     VLWRAAPGVVGALGPCREGWCHIDIAGKGGFIREDQIWGSGAL"
     gene            24151..25197
                     /locus_tag="VFF98_02680"
     CDS             24151..25197
                     /locus_tag="VFF98_02680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZU62562.1"
                     /translation="MTDTASHPAAAPDRRLATRPRVIVTRRLLPETQSRMAELFDVEL
                     NADDRPMTRAALVDAMARGHVLVPTIADRIDGEMIAEACARATDGPRVGLIANFGAGI
                     EHIDLAAARAHKIIVTNTPGVFTEDTADMTMALIVSVARRFGPGMQVLREGHWGGWAP
                     STLLGHRISGKRLAIIGLGRIGQAVAHRARGFGLQVAYHNRHRLPTAVENMLAVQYEP
                     DLDRLVAEADILSLHCPAGAATRHILSAQRIGAMKPEAYVINTSRGELIDEDALIAAL
                     ESGRLGGAGLDVFEHEPAVSPRLQALPNVVLMPHMGSATFEGRQASGEKVIANIRFWA
                     DGHRPPDQVLEGWA"
     gene            complement(25268..26674)
                     /locus_tag="VFF98_02685"
     CDS             complement(25268..26674)
                     /locus_tag="VFF98_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZU62563.1"
                     /translation="MTFKGLKPILYGGREVWPLVEGGKGVAVSNHASSGAWAAAGGIG
                     TVSAVNADSYDEDGAIIPQIYRALSRAGRHEELIRYAIDGAVEQVRRAYDIASGKGAI
                     NINVLWEMGGAQAVLEGVLEKTRGLVTGVTCGAGMPYKLSEIAARFNVNYLPIVSSGR
                     AFRALWKRAYHKVSELMAAVVYEDPWLAGGHNGLSNAEDPTQPQDPYPRVKALRETMR
                     AEGVSDDVPIIMAGGVWYLREWEHWIDNPELGAIAFQYGTRPLLTEESPIPQAWKDHL
                     RTLEPGDVLLHKFSPTGFYSSAVKNPFLLGLEARSLRQIPYSRVEAGEHTVQLDVGVK
                     GKNFWVAPRDLERARGWVAQGFAHALRTPDDTVVFVTDAEREEIRKDQADCMGCLSHC
                     GFSAWKDHDDWSTGRLADPRSFCIQKTLQDIAHGGEVDRNLMFAGHAAYRFRQDPFYS
                     NNFTPTVKQLVDRILTGD"
     gene            complement(26873..28000)
                     /gene="prfB"
                     /locus_tag="VFF98_02690"
     CDS             complement(26873..28000)
                     /gene="prfB"
                     /locus_tag="VFF98_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZU62564.1"
                     /translation="MRAEGQAHIDRIEAALALVRKFLDWERALRRFDELNARVEDPKL
                     WDNPREAEAVMKERRRLEASIGTVNEIGAEMRDAIEFIELGEMEGDDATIEEGLATLA
                     ALAARADADKVQALLAGEADGCDTYLEVHAGAGGTESQDWAQMLQRMYTRWAERRGFR
                     VELVDYHEGEQAGIKSATLLIKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSV
                     WVYPVIDDNINIEINPADLRIDTYRASGAGGQHVNTTDSAVRITHIPTNIIVASQIDR
                     SQHKNREIAMNMLKARMYEEEMRKREEAASAEHAAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVTSPTPSDVLDGDLDPFMAAALSQKVTGEKVAVEDVD"
     gene            complement(28120..28668)
                     /locus_tag="VFF98_02695"
     CDS             complement(28120..28668)
                     /locus_tag="VFF98_02695"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU62565.1"
                     /translation="MIRFSTLVRPLALAAAALAPLSAHAELAVGAAAPDFATQGAKGG
                     STFSFHLAQALKHGPVVLYFYPKAFTQGCTLEAHAFAEAAKDFHRAGATLLGLSADDL
                     PTLKRFSTEECRSAFPVAVAGRPVIAAYDVALKRDGVDTGLTSRTSYVITREGRVAMA
                     YADMDWHEHVTRTLAAVRALRH"
     gene            complement(28734..31277)
                     /locus_tag="VFF98_02700"
     CDS             complement(28734..31277)
                     /locus_tag="VFF98_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZU62566.1"
                     /translation="MTEREPDETLSYRIRRNASGLLARMNATGAGRWYARRWAHSRPF
                     RLANYALLAMALAYALVWGLVVRNLPSADKLVSYQPPLPTMVRGADGGIIYSYARERR
                     VQLRYVDFPPQLVHAFLSAEDKNFFNHGGVDFVGLGQAVVDYALKFGTGERARGGSTI
                     TQQVAKNILIGNEYSVSRKLKEMLLARRIESVLGKEQILELYLNEIPLGRQSFGVQAA
                     ARAYFGKDVGDLALHEAAFLAILPKAPETYSRAKFADKAIARRNWVLEQMVKNGYISQ
                     AQADNAKAQPLGLVNRHIDSYDPSVGYFVEEVRRRLLDQYGEGADDGPNSVYAGGLWV
                     RTSLDPELQKSAQNALAAGLLRYAAGRGWHGPIAHVELDRDNWQGQLNALNKSVSYQN
                     WRVGLVLQRGGGQGQIGFGDGFTAQLTGIPDGVRVGDVVAAAPVNAGLYAVRIVPGVS
                     GGMLVEAPESGRVLAMAGGFDFGLDSFNRATQAQRQPGSTIKPFVYATALDNGMTPAT
                     MVPDQAFCVFQGAALGQKCFKNYGDEGGGGIHTMRWGLEQSRNLMTVHIASDTGMDKV
                     VGTFKRMGIGTYPPYFSYALGAGETTVAQMVNAYAALADNGLQHDQSLIDYIQDRAGH
                     VIWRADSRKCQGCDMGEWDGKPMPRFAQRGHVVMEPTTAYQVVHMLEGVIIRGTGTAL
                     RDLNLPLFGKTGTTSGPTNVWFVGGQQTMIGGVYMGYDQPRPLGGNVQGGTFAAPIMK
                     QFIADTRPRWSHTPFIAAPGIQMVRVDRVTGQRVFDGTPSDDIKAEVIWEAFKPATAP
                     QRPTREQQLVARRQELLDEIRKGFLVLTGQSGELQKEEAPGDLTQDTGP"
     gene            complement(31355..32233)
                     /locus_tag="VFF98_02705"
     CDS             complement(31355..32233)
                     /locus_tag="VFF98_02705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZU62567.1"
                     /translation="MPRVLSIAIVVLLPVALLALVLTANQVIGSGVLRRDYVVRLQLA
                     PAGGTLGLPRVAGPMDARLPLVVLDAGHGGHDPGAGNGPLQEKALTLALARALAERLL
                     ATGHFRVALTRADDRYLALEERSGIARQLGADLFVSIHADSADAADARGATVYTLSAR
                     GSSEEAEKIAARENGADRVSGVPLRSASASAGALLVDLSQRRSAALSAAFARDVLAAG
                     AGRLGFRARPVQSAAFVVLKTPDVPSVLVEAGYISNAEDAARLASDAGRQAFADALAR
                     AMQAYFAERAGLAGPQ"
     gene            33029..>35323
                     /locus_tag="VFF98_02710"
     CDS             33029..>35323
                     /locus_tag="VFF98_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZU62568.1"
                     /translation="MTTRMLIDARHPEETRVAVLKGNRIEEFDFDSAEHKQIKGNIYL
                     AKVTRVEPSLQAAFVDFGGNRHGFLAFSEIHPDYYQIPKEDREALLAEEAAHAEEEAA
                     LRAVAEGEEDFLGDDALGEGLADDLAGGSVEDAGTLIEVDTSEKDSVATIEAGLVEEG
                     FDESHAAEEAAEDEIGADGRPRRGRRQGRAQSKQADEIRAKRMALRRRYKIQDVIHRR
                     QVLLVQVVKEERGNKGAALTSYLSLAGRYCVLMPNSSHGGGISRKISSVADRKRLKSI
                     IAEMDLPKSMGCIVRTAGLQRTKTEIKRDFDYLARLWDDIRETTLKSSAPALIHSDSD
                     LIKRAIRDIYNREIEEVVVEGDDGYRDARDFMKLLMPSHARRVKHYADPVPLFQRYGA
                     EDQLSAMYDPVVQLKSGGYIVINPTEALVSIDINSGRSTKEHGIEQTAVATNLEAARE
                     ISRQLRLRDMAGLVVIDFIDMEYGSNVRKVEKAMKEALKNDRARIQVGRISSFGLMEM
                     SRQRLRTGVLEATTRACPHCDGSGLVRTAGSAGLSALRLIEDEAAKGKGIIVSLFASQ
                     EAAIYVLNTKRADLAEIEERYGVNVEVIPEGENEGAKMRVVSSGPRNEFVPRFEAIAF
                     EEEEDDFVEEEEDEIEEAEGGSRGDDGEGDGNGRRKRRKRRRGRGRDRREEEAEDGAD
                     EGEAAGDEAEADEAVDGEAADGETGEAVDGGEEGDETPRKRRRRGRRRRGGRERREGA
                     EGAEDGVNEDAGDEAEAAEAEAEAP"
BASE COUNT         6380 a        12002 c        11514 g         5427 t
ORIGIN      
        1 cacagcggcc gccccgggtt cagcccggcc gccaccatgc gccccagcac gcctgccgcg
       61 cccagcagca ccgccgccag ccccagcccc agcagcgcca gcgcgggaaa caccagccag
      121 ccaaacacca gccccagcgc cagcgccagc acgccggcca ccgccatgct gaggccattg
      181 ttgccgctgg ccagcagcgc cggggcaaag cgccgcgcca gccaggcggc caggcgcgtg
      241 cccggcggct ggcgcgccac atcgggcgca tgcagctgca gaaacgccgc ttctgcctgc
      301 tcggccgcgg cctcgtcgcg cagcagggcc cagccgccgc catgggcggc atcggccggc
      361 agcggctttt gcgccacgcc cgcctgcagc gccacccgca gcagcgccga aggcacatgc
      421 acatcggccg gcaaatccgc caggcgcgac accagccggc ccggcagcag catggcgccg
      481 gccgcggcat ggttgaggtc tatccgttca aaacctgtgg cgaggccggt ttccaccggc
      541 tgcaccagca ccgccgggcc cgcttccagc agcggcacga tcacctccgc gcgggccagc
      601 aggccatcgg ccagcacgat cacctcgtcg gccgcgctca cctgcgccag cagggcatgg
      661 gcattggccg cccgctgcac gcgcaggcca aacagcgccg cctccccctc gacatcgccc
      721 agcggcgggc catcggcctc gtgcaggcac agcaggcgct gcacccccag cgcccgggcc
      781 agcgccagct gatggcgcag cagcggcacg ccggccaccg ccagccacgc gcgcagcccc
      841 cccgcgccag acggctgggg caccgtttcc accatcgaaa gcaaggcgat acgcagcagc
      901 tgtctcctcg cgggaaagcg ggaggccgag atgtagaggc cgcatcgccg cctgccaagc
      961 ggtaaccgcc tcggatggcg agctattcgg tgccggccag ctgcccatgc agggcgcgca
     1021 gccggcgcag cacgcccggc tcgccagggg cgccgcccag cgcttcctcg gccagctcga
     1081 tcagcggcca ggcctggaag ctggccccca gcccccgcag ccgcagcgcc gccacctgcc
     1141 agttgccatc gcagcgcgcg cggcccagca ggtctatctg ccgggcaagg ctgtcgagaa
     1201 agctggcgcg caattcgcgg aacagcgctt catcgtcgcc ggccgcggcc gcgatcatcg
     1261 catcaaggct tcccgtttcg cacaccattg cacagctgtg gcacatcagg ggttaaaccg
     1321 gggtttaaac gtgctattca gggccgatca ttcgaaaatg ccgggggaca aggcgaagat
     1381 ggccgcgcag gaacagacgg aaacggcgct ggtggccccg ctgatagaag aggcgcccgc
     1441 gccgacaccc gaagcccccg cgctggtggc cgaaccggaa agccctgccg acctgcccga
     1501 gctggaagcg ccgcaatggc cggtgcggct ggcgcaaggc ttggcgctgg ctgccctgct
     1561 ggggtggacg gggctggcgc tggccaccca tctggccgcg ctggcagatg ccgcgcaatg
     1621 ggccgcgttt gtgggcctgt gggcgccgcc ggcgctgctg ctggtgttgc tggtgctggc
     1681 ggtgggccgg ctggggggcg gcgccgggcc gcgcctgctg gtggccacgc gcgcgctcga
     1741 tgccgaggcg gcgcggctgg agcagcgcct ttcggccatg aaccgcgagc tgagcctggc
     1801 ccgcgaattc ctcgccagcc aatcgcgcga gctggagacg ctgggccggc tggcggtgga
     1861 gcgggtgaac gaacatggcg ccaggctggc cggctatgtg gaaggcagcg ccacccatgt
     1921 gcaggtgctg ggcaccgtca gcgaggcggc ggcccataat atggagcgcg tgcgccagca
     1981 tctgccggtg ctcacgcagg ccgcccgcga tgttgccaac cagatcggca atgccgggcg
     2041 gctggcggaa aaccacgtgc aggaactggt caacggcttc accaagctca acgccttcgg
     2101 gcaggccggg caaagccatg tgcaggcctt tcaggagcag accgaggcca cgctggccgg
     2161 gctggatgcg cgggtggcgc agctggccag ccagctggaa gggcggatcg cggcgatgcg
     2221 cgggctgatg gccgacgccc atggcgccat cgccgcgcgc gaggaagccg ccggcgccgt
     2281 gctgggcgag cggattgccg cgctgcaggc ggaaattgcc gccgcccgcg ccacgcttga
     2341 tggcgaggag gcgcagagtc tcacttcgct gcgcgcgcgc ctctcggccc tgcgcgacga
     2401 aagccagacc atcggccggg ccctgcgcga tcaggaacac acggcgctgg gccagtggcg
     2461 cgccgaggcc ggcgagatgg ccgccgacat cgcccggcgt cagcgcgcac atctggccga
     2521 cgccgaaacg ctggcggcca cgctcgacac cacccgccag cggctgaccg acatcgaggc
     2581 gcggctgacc gccattggcg ccagcgatgc cgcgctcgac agccgcctcg tcgcccggct
     2641 gggccaggtg gacagccggc tggccgatac cgaggcggcg ctggcgcggc tgggcgaggc
     2701 cggcgcggcg ctggtggccc gcatcgaggc cggcgccgag gccagccaga cggccctgcc
     2761 cgcggccctg gcgcaagcgg cggaagatct cgccgcctat gaacgccgcg tgttcaccct
     2821 gcgcgatgcc atgcgcgatg ccggcgagcg cggcgcggcc ctgtcgcaat ccgtgcaggc
     2881 ctcgcgcgag gtgctgatga gcgcggccgg cggcctcagc gggctgcatg acgggctggc
     2941 cgcccgggtg caggaacatg ccgccagcct tggcctgctg cgccagtcgc tcgccgggct
     3001 ggacagcgag gcggcgcgca ttgccgccac ttctggcgag gcgctggaag gggcgctggg
     3061 caggctgtcg ggcggggtgc tcgatgcggt ggcgcagatc gagctgcgcg gcgcacgggc
     3121 agtgaacgag ctggccgagc ggctgcgcag cgaaagcgcc gcggcgatcg acagcgccac
     3181 cgaggaccgc gcccgcgcca tcagcgccgg cattgccgag gccgcccgcc atgccgccac
     3241 gctgggcagc gagacggccg agcgcctgcg cgggcaactg gccgccgtgg aagatctgct
     3301 gggccatctg gagcagcgcc tgacagaagc gcgcgaggcc gccgaggaga aggtggacaa
     3361 cgacttctcg cgccgcgccg cgctcatcac cgaaacgctc aattccgccg cgatcgatat
     3421 tgccaaggcg ctggatgccg atatcgccga caccgcctgg gcggcctatc tgcgcggcga
     3481 ccggggcatc ttcacccgcc gcgcggtgcg cctgctggat accgccgagg cccgcgccgt
     3541 gctgctgagc tatgagcgcg accgcgcctt tgccgagcac gtcaaccgct tcatccatga
     3601 tttcgagggg atgctgcgcc agttgctctc cacccgcgat ggccatgcgc tgagcgtgac
     3661 gctgctgtca tccgacatgg gcaagctgta tgtggccctg gcgcaggcta ttgaacgcct
     3721 gcgcagctga ttgggccttc agccctcgct gctgcccagc gaggcgggcg ggtggaggaa
     3781 gctgatatcc tccacgctca gccagttatg ctggcggttc agccagaaca gcgcgaaggc
     3841 caccgccgct atcgccgtgg cgcgcaaggc cacccggccg gggcggaaat tggccggcgc
     3901 gctgtcggcc tggccgggca ccagcggctc gcccacctcg gcatgggtgc gcacgccgaa
     3961 aggcagcacg aacaaagcgg agatcaccca gaacagcagg tagaccgcca gcatcgagct
     4021 ccacttcatc cggccccctc ccctttatcc ggtcagtcca gcagcatcac ccgcacctgc
     4081 gggcgcttgc cgcaccagcg ctgcgttgcc cggcgggcgg ccaggcgcgc ggcttccttc
     4141 agctcttcca catcgcgccg ggccggcttc ttgtcgagcg cgcgggcaat atcctcgcgc
     4201 gcttcggcca cgaaattctc gtaatcctcc accagcggca ggcccaggcc cagcacgtcg
     4261 atcctgaggt ggctcttgtg cttgccgcca tggccggaaa ggcccgccag ccccaccacc
     4321 accacgccat tatgcgccat gcgccggcgc atcagcaccg cctcgccatc ggccggggca
     4381 atgatgtcgc catccagcac cagccggccg gcgcgcactt cggcaaactt gcccggccgg
     4441 cccggcgcca ggcgcaccag atcgccgttc ttctggttga cgatgcgggc aatgccgctg
     4501 gccttgccca gccggccctg ctcgcgcatg tggcgctcct cgccatgcac gggcacgagg
     4561 atctccggcc ggatccagcc atacagcgcc tgcagttccg gccgcccggg gtggcccgag
     4621 acgtggatgt ggctctgccg gtcggtcacc acggtgatgc cctgcgcggc cagctggttc
     4681 tggatgcggc cgatggggat ctcgttgccg gggatctgcc ggctggaaaa cagcacgaca
     4741 tcgcccgctt ccagcgagat cgggtggttg ccctcggcaa tgcgggcaag ggcagcgcgc
     4801 ggctcgccct ggccgccggt ggccacgatc agcacctcgc cgcgcggcag ctccatcgcg
     4861 gtgtcgacat ccaccagatc gggcagctgc ttcagatagc ccgaggcctt ggccgcatcg
     4921 atgatccgat ccagcgagcg gcccgagacg cacaggcgcc gccccgtctc gcgcgccacc
     4981 cggcccagcg tttccagccg cgcgacgttg gaggcaaagg tcgtcaccag cacacgcttg
     5041 cccttgtggc ggcgcacttc ctccagcatc gcccggccca catccccttc ggaacccgag
     5101 gggttggggt tgaacacatt ggtgctgtcg cacaccagcg ccagcacgcc ctcgtcgcca
     5161 atccgggtca gctcctcggg gctggccggg cggcccacca gcggctcgtc gtccagcttc
     5221 cagtcgcctg tgtggaagat gcggccaaag ggcgtgtcga tcaccaccgc attgccctcg
     5281 gcgatggaat gggccagcgg cacatagcgg atggaaaagg gcccgacgcg gaacggatcg
     5341 ccatgatcga taacgatcag ctccacctca ccggccagcc ccgcttcctc cagcttggcc
     5401 gctaccagcc gcgcggtgaa cggcgtggca tagagcggca cgccaaggtc ggcggcgaaa
     5461 tagggcaccg cgccgatatg atcctcatgc gcatgggtga gcacgatgcc ggccagcgcc
     5521 tcgcggcgct cctcgatgaa atcaaggtcg gcgaacacca gttccacgcc cggatattca
     5581 ttgccactga aggtcatgcc cagatcgacc atcagccact ggccctcgca gccatagaga
     5641 ttgacattca tgccaatctc gccggagccg cccagcgcgc aaaacagcaa ttcgttaccg
     5701 ggaatatgcg caagctttga gatcttacct ctcctgacag ttttcggcgg tggtttagcg
     5761 cgccgccgcg cgagtggcca gcaaggccag gccgttcaat gtaaggtcgg ggtccaccgc
     5821 gtcgaacagg ccggtctcgg cgttgaacag cacggcaagg ccgccggtgg ccaccaccgt
     5881 cgccggccgg ccgatctgcc ggcgcatgcg cgcgatcagc ccctcgatca tcgccacata
     5941 gccccaatag atgccgatca gcatctggtc ttcggtgttg gtgccgatga cgctggggct
     6001 ggcgggccgg gcaatggcaa tgcgcggcag cttggcggtg ttgcccacca gcgcatcgag
     6061 cgacaggttg agccccggcg cgatgatgcc gcccttgtag gcgccgttga aatcgatcgc
     6121 atcgatggtg gtcgccgtgc caaaatcgac gacgatcaga tcgccggcat atctggcatg
     6181 ggcgccaagg gcgttcaccg cccggtctgc ccccagcgat ttgggatcat cgacatcgat
     6241 atcgatggca tagcccgcct tgccctggcc ggccaccagc ggctccacat cgaaatattt
     6301 gctgcccagc acctgcagat tgtgcagcgc gcgcggcacc acggtggaaa tgatgatctg
     6361 cgccacagcg ccgcgatcaa acccctcgat cgccagcagt tgcatcagcc acacggcata
     6421 ttcatcggcc gtgcggcgcg gatcggtggc aatgcgccag cgcgccttga tcgtctcgcc
     6481 gtcgaacagc gcgaagacca cgttggtatt gccgacatca atcgccagaa gcattggtcc
     6541 accccgttta tgcgcaaagc tctgcggctt ttatcacagc agcgcaacat cgcctgcatg
     6601 aatggcacgg attgcgccat cggccaatcg caagcgcatg gcgccatcct cagccaggcc
     6661 cgcaaagcgg ccctcgatgc gctggccatc ggcccctgtc acgcccagcg gcgtgccctg
     6721 cggcagggcg gcctgctgcc agcgggccac cagcgcggcc agcccatgcg cgcgccagct
     6781 ggccagctgc gcggcaaacg ccgccgccat ctggccggca aaatgatcga gcgcgggcgg
     6841 cggggcgaaa tcggccagcg ccacggtttc gcggccttcc agcagcgggg cgctggcaag
     6901 gttcaccccc acccccacga tcaccgcatc gccctgcgct tccagcagca cgccggcaag
     6961 cttggcgccg ttcaccagca catcgttggg ccatttgagc aagggggcca gcggcggcgg
     7021 cacatggccg gccacagctt cggccacggc cagcccggtt aacagcgcaa ggctggccgg
     7081 cggcggatcg cccggccccg gccgcaccag cgtggagccc atgaaattgc ccgcgccatc
     7141 ctgccaggcc cggcccaggc ggccgcgccc ggccgtttgc cggcgggcga ccagccattc
     7201 cccctcaacc gaaggcgcgc cagcagcaag ccggcgcgcc cattcggcgt tggtggaatc
     7261 gatctcgttg aaaaaggaga tcaagcggcg atcacgccgt gtggaacagg gctgcggcgg
     7321 cctgttgggc caccccgccc agccagttcg tcatcagata gcccaccggc gagatgacca
     7381 ccgccgaaac cagcaggatg gcattatgcg ccacatcgct ggccggggcc accttgtcgg
     7441 ccggatcgtc gaaatacatc accttgacga ccttcagata gtagaaggcg ccgatgacgc
     7501 tggcggcgat gccgatggcg gccagcggca ccatgccagc tgccaccgct gcctggaaca
     7561 ccacgaactt gccccagaag ccgaacagcg gcggaatgcc cgccaggctg aacatcacca
     7621 tcgccagcac cgcggccagc gccgggcgcg tgcgcgagag gccggcgata tcggccaggc
     7681 tctcgatctg ctcgccctcg cggtcacgca gcagcagcac ggcggcaaag ccggcaaacg
     7741 aggtgaacac atagatgaac agatagacca gcaccgccga cacgcccgcc ggcgttgccg
     7801 cggcaaggcc gatcagcatg aagccgacgt tgttgatcga cgaataggcg agcaggcgct
     7861 tcaggttcgt ctggccgatg gcgcccagcg cgccgaccac gatcgacagc aacgacaggg
     7921 caatgacgat ctgctgccag gccaccactt cggcgccgaa agccaccagc gtgacacgca
     7981 tgaggaggca gagcgcggcc accttgggcg ccgtggcgaa gaaggtcgtc accggggtgg
     8041 gcgaaccttc ataaacgtcg ggcgtccaca tgtggaaggg cacggcgctg atcttgaagg
     8101 ccaggccgac cagcatgaac accacgccga acagcgcgcc agtggccagc tggccatgca
     8161 gcgccttgcc gatggcaacg aaattggtgc tgcccgagaa gccataggtc aggctcatgc
     8221 cgaacagcag gatgcccgag gccagcgcgc ccagcacgaa gtacttgagg cccgcttcgg
     8281 ccgaacgctc gtcacgacgc agcatcgccg ccagcacata ggccgccagc gagttgagct
     8341 cgaggccgat gtaaagcgtc agcaggtcgg tggccgagac catcagcccc atgcccagcg
     8401 cggcaaacag caccagcacc ggatattcgg cccgcatttc atcgcgcggg gcgaagaagc
     8461 gcggggcgac gatcaccgcc acagcggcgg ccagatagat cagcagcttg gcataagcgg
     8521 caaagccatc gtcggcatac tggccataga aggccgaggt ctgcgcgccg gtggcgccca
     8581 ggccctgcag cacaaaggcc gaggcggcca ccagagcggc cacggccgcc accgagatcg
     8641 cgcgggcgga tttatcaccc gcccaggccg cgaccagcat caataccaag cccgccgcgc
     8701 tgaggatttc ctccgcggcg accaggttga gggacacaat ccagctcatt aacgcgcact
     8761 cccctcaacg gtattggcag cggcttcggt tttcggcgcg ggcgtgccgg ccgtaggggc
     8821 ggcatcgctc gcgggccggg ccgaggccag gcgcgcatcc agcgcggcga tatcggcgcg
     8881 catcggcgcg ataaagcttt cgggatagat gcccatccac agcgtggcgg ccaccagcgg
     8941 cgccagcagg gcccactcgc gcaggtcaag gtcaggcatg gcggccgcat cggcgttctt
     9001 ctgcgcgcca aacaccaccc gggcatagag atacagcatg taggccgcgc cgaggatgat
     9061 gccggtggtg cagatcagcg ccgcccaggt cgacacctga tagatgccgg ccagcgagag
     9121 gaactcgccc acgaagttgg cggtggccgg caggccgatc gaggccatgg tgaagaacat
     9181 gaagaacagc gcatatttcg gcatgttgat ggcaaggccg ccatagcgcg agatctcacg
     9241 ggtatgcagg cggtcgtaga tcacgccgac gcacaggaac agcgcgcccg ccaccaggcc
     9301 gtgcgccagc atcaccacca tcgagccttc caggccctgg cggttgaagg agaacaggcc
     9361 gatcgtgacg atccccatat gcgccaccga ggaataggcg atcagcttct tcatgtcctg
     9421 ctgcacaagg gcaaccaggc tggtgtagac caccgcgacc atcgacaggc cccacaccag
     9481 cggcgcgaaa taggcgctgg cttccgggaa catcggcagg ctgaagcgga tgaagccata
     9541 gccgcccatc ttcagcagca caccggccag gatcaccgaa ccggcggtgg gcgcctgcac
     9601 gtgggcatcg ggcagccagg tgtgcaccgg ccacatcggc atcttcaccg cgaagctggc
     9661 aaagaaggcc agccacagcc aggtctgcgc cttcaccggg aagttgtaga ccatcagcgt
     9721 cgggatatcg gtcgtgccgg cttcatgcac catccacagc atggcgatca gcatcagcac
     9781 cgagcccagc agcgtgtaga ggaagaactt gtagctggcg tagatccgat cggcgccgcc
     9841 ccagatgccg atgacgagat acatcgggat gaggccggct tcgaacatga tgtagaacag
     9901 atagagatcc tgcgccgcga agacgccgat catcagcgtt tccatcagca ggaaggcggc
     9961 catatattcc ggcacgcgct tctcgatcgc atgccagctg gcgccgatgc agatcggcat
    10021 gagcagcacg gtcagcgcga tcagcgtgag cgcgatgcca tcgatgccca gcgcccactg
    10081 gaagccggag aacagcggca gcctttcggt gaactgccac tgcgggccac cgatctggta
    10141 gtgctgccac agcaccacac ccagcaccag atcgacaagg gtggcgccca gcgccagcca
    10201 gcgcgccccc tgtgccgata gcgccaggct gaccagcgat gccaccagcg gcaccgccag
    10261 catcagcgaa aggattggaa aaccgatcat tagctcttag cccttttcgc cgtttgcctt
    10321 accgaaccga ctgccacatc acccagctga ccgcggcaac gaggcccaga agcatcgtca
    10381 gcgcatagct ggtgagataa ccggtctgca gcctgcgcgc gccggccacg cccttggcca
    10441 ccacggcggc agcgccgttg gggccgaagc ggtcgatgat gccgatatcg cccagcttcc
    10501 agaacgcccg gcccagcacg aaggcaggac gcacgaacag gaagttgtac agctcgtcga
    10561 agtaccattt gttgtagaag aatgcgtgga gcggcttgaa caccttgacg aaggccgccg
    10621 ggatcgaggg cacctgcaca tagcagaccc acgcgccgac aagaccggcc accattacct
    10681 cgaagggcgc ccacttcacg gctgccggaa tatgctcggc cgcctcgaat agctccttgt
    10741 cgaagaacag ggtgctggcc ttccagaaat cgccagcggt ttcgatgccg acgaaatagc
    10801 ggttgaacag cgcgccggcg aacaccgccc ccagcgtcag cacgatcagc gggatcagca
    10861 tgttgagcgg gctctcatgc ggatgatagc cggcggtgcc gtcgtcctca tggccgtggt
    10921 ggccatgggc gtgatcgtga tcatggccgt gatgggcatg gtcgtcatgg ccatgggcat
    10981 catgcacggc gtgctggata tgctccgact ggttccagcg cggcttgcca aagaaggtga
    11041 ggaacaccag gcgccacgag tagaagctcg tcagcaaagc ggcaacgatg ccggtgatgc
    11101 tggcgaactg gcccagggtg gtgccgcggg caaaggccgc ctcgatgatc gcgtccttcg
    11161 agtagaagcc ggcaaaaccg cccacttcag ccaggcccac gccggtgatc gccagcgtgc
    11221 cggccatcat cgccacgaag gtcagcggga tcttcttcca caggccgcca tagtagcgca
    11281 tgtcctgctc gtggtgcatc gcatggatga ccgagcccgc acccaggaac agcagcgcct
    11341 tgaagaaggc gtgggtgaac aggtggaaca tcgaggcgcc ataggccccg acgccagcgg
    11401 cgaagaacat gtagcccagc tgcgaacagg tggaataggc gatcacgcgc ttgatatccc
    11461 actgcgtcgt gccgacggtg gcggcaaaga agcaggtggc agcgccgatg aaggtgatga
    11521 cgtgcagcgc cgtctcgctc tgctggaaca gcgggctgag gcggcagacc atgaagacgc
    11581 cggccgtcac catggtggcg gcgtggatca gcgccgacac aggcgtcggg ccttccatcg
    11641 cgtcgggcag ccaggtgtga aggcccagct gcgccgattt gcccatggca cccacgaaca
    11701 gcagcaggca cagcagcgtc agcgtatcca cccggtagcc caggaagccg atggtgctgc
    11761 cggcataatc gggcgcatgg cgcaggatcg tctcgatatc gacggtgtgg aacaccagga
    11821 agatgccgaa gatgcccagc atgaagccca gatcgcccac gcggttgaca acgaaagcct
    11881 tgatcgccgc cgcgcaggcc gagggcttct ggaaccagaa accgatcagc aggtacgagg
    11941 ccaggcccac gccttcccaa ccgaagaaca tctgcaccag gttgttcgcc gtcaccagca
    12001 tcagcatggc aaaggtgaac agcgacagat aggcgaagaa gcgcggctga tccggctcct
    12061 cgctcatgta gccccaggaa tagaggtgga ccagcgccga aacggtggtg atcaccacca
    12121 gcatcaccgc ggtcaacgcg tcaacgcgca gttcccagtt gaagacgaga tcggccgagt
    12181 ggacccactg cagcaccggc gtgaccgaca cttcggcgtg gccgcccaga aactggcaga
    12241 agatcgacca ggagagcgcg caggcggcaa acagcgcgcc cgtcgtcacc agcttggccg
    12301 gcaccttgcc gatggccttg ttgccaaggc cggcgataac cgccgccaac agcggcagaa
    12361 atacgatgaa caggatcgga tgcactggcc ttatcccttc atccggttga cgtcgtccac
    12421 cgcgatggac ccacggctac ggaaatagat gaccagaatg gccagaccca cagctgcctc
    12481 gccggcagcc acggtgagca cgaacatcgc aaacacctgg cccaccagat cgtggaggaa
    12541 ggcgctgaaa gcgacgaggt tgatgttcac cgcgagcagg atcagctcga tggccatcag
    12601 gatgatgatg atgttcttgc ggttgaggaa aatccccaga acgcccagca cgaagaggat
    12661 cgagctgacg gcgacgtaat gttcgatacc gatcatggct gcccccttca gatctgcacg
    12721 ccctcgccca cggcggggcg agtgttgaca gtggcgtctg ccggacggcg cgacacctgc
    12781 ttccagatgc tctgcgtctt cacgcccgta cgctggcgat gggtcagcac gatggcgccg
    12841 atcatggcga ccagcaggat gatccctgag ccttcgaaca ggaagagata gcggccatag
    12901 agcagcgcgc cgatcgactg gatgttgctg aggcccgcct gcggggcggt tgccggatcg
    12961 gccacgccca gttgcagccc gccggcatgc caggcgccaa ggcccatgcc cagttccgcc
    13021 agcagcacca ccgcaatggc caggcccagc ggcatgttgc gcatgaaacc ggcgcgcagc
    13081 gtggcgaaat cgatgtcgag catcatcacc acaaagagga acagcaccgc caccgcgccg
    13141 acatagacga tcaccagcag catggcgatg aactcggcgc cggccagcac catgaggccg
    13201 gccgcgttga aaaaggcgag gatcagccag agcaccgagt ggaccgggtt cttcgcgaag
    13261 atcgtgaagg tgcccgatgc caccaccagc gtggcgaaga gataaaaggc aagagtttca
    13321 atcatggccg cgcccttacc gatagggcgc atcggcttca aggttggcag cgatcgctcg
    13381 ctcccacttg tcgccgttcg ccagaagttt cgccttgtcg tagagcagct cttcgcgcgt
    13441 ttcggtcgcg tattcgaagt tcggcccctc gacgatggca tcgaccgggc aggcttcctg
    13501 gcagaagccg cagaagatgc acttggtcat gtcgatgtcg tagcgggtgg tgcgccggct
    13561 gccatcctcg cgcgggtgcg cctcgatggt gatcgcctgc gccggacaca ccgcctcgca
    13621 cagcttgcag gcaatgcagc gctcctcgcc gttgggataa cggcgcaggg catgctcgcc
    13681 gcggaagcgg ggcgacagcg ggttcttctc gaagggatag ttcaccgtcg ccttctcctt
    13741 gaagaaatac ttcaaggtga gcgcgtgcgc tttcacgaat tcccagaggg tgaagctctt
    13801 gacgagctga ccgacactca tgcgaaatgt cccgtagcca tgaggtagcc gctgacgagg
    13861 aacacgaaca gcagcgagac aggcaggaaa accttccagc ccagacgcat cagctggtcg
    13921 tagcgatacc gcggcacggt ggccttcacc cagctgaaca caaagaaacc aaagcccatc
    13981 ttggcgatca gccagatcca gcccggaatg agatagagcg gcgcccattc caccggcggc
    14041 agataaccgc cccagaacag cgtggcattc agcgcgcaca tcagcagcac gttgccatat
    14101 tcacccagcc agaagagggc gaagctcatc gaggaatatt cggtctgata accggccacc
    14161 agctcgcttt cggcctcggt caggtcgaag ggggcgcgct gggtttccgc caggctcgag
    14221 atgaagaaca tcacccacat cgggaacagg aagatgttgc aggcaaaggc gttgatgaag
    14281 cccagcccat gccccttctg tgcgatgacg atgctgttca tgttgaacga gccggcccac
    14341 agcaccacgc agatgacgat gaagccgatc gagacttcat aggagatcat ctgggccgag
    14401 gcgcgcatgg ccgaaaagaa cggatatttc gagttcgacg cccagccggc catgatcgtg
    14461 ccgtagacac ccagcgagct gatcgccagc acatagagca cgccgacatt gatgttggcg
    14521 agaatggcgc ccgagttgaa cgggatcacc gcccaggcca gcagcgccac cgtgaaggtg
    14581 acgatcgggc cgatgaggaa caggcccttg ttcgccgccg aaggcacgat ggtttcctgc
    14641 aggaacacct tgaggccgtc ggcaaagctc tggaggatgc cgaccgggcc gaccacgttg
    14701 gggccgcgcc gcagcgccat cgccgcccag atcttgcggt cgaaatagat caccagcgcc
    14761 accgacagca tcaccggcag agcgatcgcc agaatgccga tgaccgtggc cagaaaccac
    14821 gcccagccat gcggcaggcc ggccatttcc agcagttgca tcggggtatg cgggaacagg
    14881 gtcattccgc ggcctccttc tgggtttcgc cgtggagcag ttcggccgag cagcgctgca
    14941 gcgtggcgct ggcccgcgcg atcgggttgg tgaggtagaa gtcggcgatc ggataggcga
    15001 tcacgcccgc ggccgcaccg gtaacgccct tgggcagcgc gccgtaatcg gccagccctt
    15061 cctgcccgaa ggccgggttg aaggcgaaca gcgcggcgcg cagcgcatca aagctgtcga
    15121 agccaacctt aacgcccagc gcatcggcca gggcgcggat gatcgcccag tcctcgcgcg
    15181 catcgccggg ggcgaacacc gccttgtcgg catattgcac gcggccctcg gtgttgacgt
    15241 agatgcccgc cttctcggtg aaggccgagc ccggcaggat gatgtcggcg acatgggccg
    15301 ccttgtcgcc atgatggccg acatagacga tcatgctgtc agcaaacttg gcgaaatcca
    15361 cctcgtcggc gcccagcgcc agcagcatct tgggcttggc cgccgcgata tcggcaatgc
    15421 cgcccttctg cgcatagccc agcatcagcg aggccatgcg gctggccgcc atgtgcagca
    15481 cgttgaagcc gttccactgg ctgccatctt ccagctcgcg cacaaggccc agctggtcgc
    15541 tgaccgacag cgccgcttcc agcgcgccct tggccaggcc agcgccgcca acgatgatcg
    15601 ccggccgctt ggcttcctgc agcgccgccc acacatgggc cggcagctgg ttgaggatgg
    15661 ccgcatccgc gccgaggaat tccgccttgt aggtgggatc ccattcgggg ccgatgatga
    15721 acaccttggc gcccttcttc acggccttcc tcaggcgcgt gttgagcagc ggcgcttccc
    15781 agcgcacatg gctgcccacc accagcaccg catcggcatt ctcgatgccg gcgagcgtgg
    15841 tgttgaagtt cacggccgcc aggttcgagc agtcgtaggc caggcccgtc tgccggcctt
    15901 ccagcagggt ggagcccagc gccgccagca gctgcttggc cgcaaacagg gtttcagcat
    15961 ccaccagatc gcccgcgacc gccgccacgc tgctgcccgg cgcgatcttg gcgacagcgg
    16021 ccagcgcctc gctccaggtc gccggcacca gcttgccatc gaggcggata tagggccggt
    16081 cgagccggcg cttggtaagg ccatcgacga ggaagcggcc cttgtcgctc agccattcct
    16141 cgttcacatc gtcgttcacc cgcggcagga tgcgcagcac ttcacggccg cgcgcatcga
    16201 tgcggatgtt ggcgccaatc gcgtccgaca cgtcgatgcc cagggtcttc ttcagctccc
    16261 aggggcgcgc ctcgaaggaa taggggcggt tggtcagcgc gccgaccggg cacagatcga
    16321 tcacattggc cgacagctcg tgcttggcgg ccttttccag ataggtgctg atctgcatgt
    16381 tctcgccgcg atagagcgcg ccgatctcat ccacgccggc aatctcttcc gagaagcgca
    16441 cgcagcgggt gcactggatg cagcgggtca tcaccgtgcg gatcagcggg cccatatact
    16501 tctcggtcac cgcgcgcttg tgctcgtgat aacgcgaggc gccgcggcca taggccacgg
    16561 actggtcctg caggtcgcat tcgccgccct ggtcgcagat cgggcaatca agcgggtggt
    16621 tgatgaggag gaactccatc accccttcgc gcgccttctt gaccatctcg ctgtcggtgc
    16681 ggatttcctg gccctcgctg gccggcagcg cgcagcttgc ctgcggcttg ggcggcccgg
    16741 gcttcacttc caccaggcac atgcggcaat tgccggcaat gctcagccgt tcgtgatagc
    16801 agaagcgggg aatttccttc cccgccagtt cgcaggcctg aagcaccgtg gcgccggccg
    16861 gcacctcgat ctcgacgccg tctaccttga ccttgggcat tactctgctg cctcctgcag
    16921 gccgccattg cgctgggcaa tacggcgctc aatctcgggg cggaaatggc ggatcaggcc
    16981 ctggatcggc caggcggccg catcccccag cgcacagatc gtgtggcctt ccacctgctt
    17041 cgtcacgttc cacagcatgt cgatttcctc gatcgaggca tcgcccacgc gcaggcgttc
    17101 catcacgcgc cacatccagc ccgtgccctc gcggcagggg gtgcactggc cgcagctctc
    17161 atgcttgtag aaatagctga ggcggctgat cgcgcggacg atgtcggtcg acttgtccat
    17221 gacgatgacc gcagcggtgc ccaggccaga acccagcgcg cgcaggccgt caaagtccat
    17281 cggcgcatcg atcatctcgg ccgccggcac cagcggcacc gaggagccgc cggggatcac
    17341 cgccagcagg ttatcccacc cgccgcgaat gccgccgcaa tgcttctcga tcagctcgcg
    17401 gaaggggatc gacatctcct cttccaccac gcagggccga tcgacatggc cggagatctg
    17461 gaacagcttg gtgccccggt tgttctcgcg cccgaaggag gagaaccagc cggcgccgcg
    17521 gcgcaggatg gtgggcgcca ccgcgatcga ttccacattg ttcaccgtcg tcgggcagcc
    17581 ataaaggccg gcgccggcgg ggaacggggg cttcaggcgc ggctggccct tcttgccctc
    17641 gatgctctcg atcatcgcgg tttcttcgcc gcagatgtag gcgccagcgc cgcgatggac
    17701 gaagacgtcg aaatcatagc ccgagccgct ggcgttcttg ccaacaaggc ccgcctcata
    17761 ggcttcggcc acggcggcaa acagggtttc ggcctcgcgg atatattcgc cgcgaatgta
    17821 gatgtaggcc gcgcgggccc ccatcgcaaa gccggccacc agcgcgccct cgatcagctt
    17881 gtgcggatcg tggcggatga tctcgcggtc cttgcaggaa ccgggctcgg attcatcggc
    17941 gttgataacg aggaagctgg gccggccatc gcggctttcc ttgggcatga agctccactt
    18001 catgccggtg gggaagccgg cgccgccacg cccgcgcaga cccgaatcct tcatttcctg
    18061 aatgatcgcg tcgcgcccct tttcgagcaa cgccttggtg ttgtcccagt cgccgcgctt
    18121 gcgggcggca tccaggttcc acggctgatg gccgtagagg ttggtgaaga tgcgatcctt
    18181 atctgcaagg gccatgactt accactcccc ccgataatcg tgattcccgc tcaccatggc
    18241 ggtgaggttc gacagggcgc cggccggctc caccgtgtgg cggccgggtt cctgcgtgcc
    18301 ggccttgggg gtctcgcccc gggccagcgc atcgagcacc ttgtcgagcc gctcgacggt
    18361 gagatcctcg taattgtcgt cgttgatctg caccatcggg gcagaggcgc aattgcccat
    18421 gcattccacc tcggtgaagg tccagaggcc gtcctcgctg acatgcccgg ccttcatgcc
    18481 gcggccatgg caggcagcga tcaggtcgtc cgagccgcgc agcatgcagg gcgtggtgcc
    18541 gcacacctgc acgtggaaac ggcccaccgg caccagattg tacatggtgt agaaagtggc
    18601 gacctcgacc acgcggatga tcggcatgtc gagataggtc gcgacatatt ccatcaccgg
    18661 gatcggcagc cagccttgcg tattggtttc ggcgcccacc tggcgctggg ccagatcgag
    18721 cagcggcatc accgcagagc gctgccgccc ttcgggatag cgggcgacga cttccttggc
    18781 cttttcggcg ttctgcgccg tccaggcaaa ggcgccccag cgggcgcgca gttccggcgt
    18841 atccggttcg agatgacgat cagccattag cggatgaact ccacgcgggc gcacttatcg
    18901 ccctcgaaag tgtagacggc gaggacgtcg aacggctcgg aagccgccgt gcgccacacg
    18961 cgctcgtgaa agacgacgta attgcccagc gccttcacat cgatgatttc cgcgcggttc
    19021 tcggggaact cggcgaacat cctgacgagg ccggccctgg tgccctcacg cccttcgcgc
    19081 accacggcgc cgcgataggc ggcctcgcag gcgtcctcgg tcatgaacga cacgtaagta
    19141 tcggcatcct gggcgttata ggcggcaatc atcgcccttg cggtgtcggc atgcgacagt
    19201 gccagcgccg cgctcaccgg tcacactccc cgaacacgat gtccatcgcg cccagaatgg
    19261 cggtgatatc gggcagcatg tggcccttgg tgaggtaatc catcgcctgc aggtggctga
    19321 aggcggtggg ccggatcttg cagcgatagg gcttgttgcc gccatcgctg acgagataga
    19381 cgccgaattc gcccttgggg ctttcggtgg ccacataaac ctcgcccgcc ggcacgtgga
    19441 agccttcggt gtagagcttg aagtggtgga tcaggctctc catcgactgc ttcatctcgc
    19501 cgcgcttggg gggcgagacc ttgcggtcgg tgctggcgat cgggccatcg ggcatttcgg
    19561 ccaggcactg cttgacgatg cggaccgact ggcgcacctc ttccacgcgc accatcatgc
    19621 gatcatagca atcgccgctg gtgcccaccg gcacgtcgaa gtccatgcgc tcgtaaacgt
    19681 cgtagggctg gctcttgcgg atatcccagg caatgcccga gccgcgcagc atcggccccg
    19741 agaagcccca gcgcacggca tcttccttgg tcacgatgcc gatatcgaca ttgcgctgct
    19801 tgaagatgcg gttgtcgacg aagagcgagc aggcatcctc gtaaagctcg gggaagcgct
    19861 tctccagcca ttcgccgata tcgacgagca gcttgagcgg cacatcctgg tgcacgccgc
    19921 ccgggcgata ataggccgag tgcatgcgcg cgcccgaggc acgctcgaag aagttcagca
    19981 gatcctcgcg gatctcgaag aaccacagca tcggcgtcat cgcgcccacg tccatcaccg
    20041 ccgaaccgat gttcatcatg tggttcgaga tgcgggtcag ctcggcgaac agcacgcgca
    20101 gatattgcgc gcgcagcggc acttccacgt tcagcaattt ttcaactgcc agcacgtagc
    20161 tgtgctccat cgacagcggc gaacaatagt ccagccggtc gaaatagggc agcgcctgaa
    20221 ggaaggtctt gtgttcgatc agcttttccg tgccgcggtg cagcaggccc acatgcggat
    20281 caatgcgctc gacgatctcg ccgtcgagct cggtcaccat gcgcagcacg ccgtgggcgg
    20341 ccggatgctg ggggccgaag ttgatcgtgt agttggtgat gatctcgtcg ccggtggtcg
    20401 gcgactgttc gagggaaagg ccgctcactt cttctcccct tccgcctttt cgtcgcccgg
    20461 cagcacgtat tccgcgcctt cccaggggct catgaagtcg aactggcgca ggtcctgggc
    20521 cagcttcacc ggctcataca ccacccgccg gtcctcttcc gaccagcgca gttcctgata
    20581 gcccgtcagc gggaaatcct tgcggaacgg atggccttca aagccataat cggtgagaat
    20641 gcggcgcagg tccgggttgc cggcgaagat cacgccatac atgtcgtaga cttcgcgctc
    20701 gagccagcct gccaccggcc acagcgtggt cacggtcggc accggcttca cttcatcggt
    20761 cgtcaccttg acgatgatgc ggtggttgcg ggtcaggctc agcagcatgt agacaacgtc
    20821 gaaacgctcg gcccggtccg gatagtcggc gccggcgatc tccatcagct gctggtattc
    20881 atgctcgtcg cgcagggcac gcagggtgtc ttccagcgtg tcgcgcgcaa cggtgatcac
    20941 cacctcgcca tgcgcttcat gggcggccag cacgctggcg cccagagcgg cggtgaggtt
    21001 gtcgatcacg ccttcgttcg aggcgaattt gggggcggaa tgcaaaacgg ccatggtctg
    21061 tttcccctca gcgctcgatc gtgccgacgc ggcggatctt gcgctgcagc tgcatcacgc
    21121 cataaagcag cgcttcagcg gtcggcgggc agccgggcac atagatgtcc accggcacga
    21181 tgcgatcgca gccgcgcacc accgaatagg aatagtggta atacccacca ccattggcgc
    21241 acgagcccat cgagatgacg tacttggggt tcgacatctg gtcgtagacg cggcgcagcg
    21301 ccggggccat cttgttgcac agcgtgccgg ccacgatcat cacgtcgctc tggcgcgggg
    21361 aagcgcgcgg cgcggcgcca aagcgctcca tgtcatagcg cggcatgttg gcgtggatca
    21421 tttccaccgc gcagcaggcc aggccaaagg tcatccacca caaggagccg gtgcgcgccc
    21481 agtggaacag gtcttccaca ttggtgacaa ggaagccctt gtccgacacc tcggcgttga
    21541 ggtcgttgaa gaaggcctga tcgggcgcga cgagctggcc ggcctgagcg gcggcggcag
    21601 gagacaattg cgtgctcatt cccagtccag agctcccttc ttccaggcat agatcagccc
    21661 aagcaccagt tcacccagga acaccatcat ggtgatccag ccggcccagc cggtttcctt
    21721 gaggctgacc gcccagggaa agagaaatgc cgcttcgaga tcgaagacga tgaacaggat
    21781 cgccaccaga tagaaccgca cgtcgaactg gctgcgcgga tcttcgaagg cggggaagcc
    21841 gcattcatat tcgctcagtt tgtcggcgtc gggctggtgg gagccggtga gacgggccgc
    21901 tgccatgggc aggaagacga aggccgatga caatcccagc gcaaccacca ggaagatcag
    21961 aatcgggaga tattgcgtaa gatcgaccaa gggctgactc gcgctactag agtgatggcc
    22021 tgccccctag tcccgcagcc gggctggcgc aagtggaggt tgggccaaga aaatgcctaa
    22081 agcgcaccag ccgttgcgcg gggtggatca ttccccccgc agcggcggat tgccattgcg
    22141 gcggcgcagc attatgccgg tcaagcaggc acgtcaaaca ttcccgattt ttctttcgcg
    22201 aatccgcttc cgacgccctg ccgaacccga gaggacaaga aggccatggc caatatttcc
    22261 gcccaagatc cggttgttat cctgtcttat gcgcgcacgc ccatgggggg cctgcaaggc
    22321 gctttggctg atgccagcgc taccgatctc ggcgccaccg cggtgaaggc cgcggttgat
    22381 cgcgccggcg tttcgcccga tgaaatcgag cgtatctaca tgggctgcgt gctccctgcg
    22441 gggctgggcc aggcgcctgc ccgccaggcg gcgctgaagg cgggcctgcc caaatccgtg
    22501 caggccacca cggtgaacaa ggtctgcggc tcgggcatgc agacggtgat catgggcgcc
    22561 gaggccctgg cggccggcag catcgatctg gtcatcgccg gcggcatgga aagcatgacc
    22621 aacgcgccct acctgctgaa gaagcaccgc tcgggcgcgc gggccggcca cgacaccgcc
    22681 tacgaccaca tgttcctcga tggcctggaa gacgcctatg aaggcgccgc catgggcgcc
    22741 tttgcgcaga tcaccgcgga tgactaccag ctgacccgcg aggcgcagga cgcctattcg
    22801 atcgaatcgc tggcccgcgc caaggcggcc gtggccgaag gccgctttgc cgaagagatc
    22861 gcccctgtca cgctgaagac gcgcgcgggc gaagtggtgg tggataccga cgaggccccc
    22921 ggcaaaggcc ggcccgacaa gatccccacg ctgaagcccg ccttcgccaa ggatggcacg
    22981 atcaccgcgg cctcctcctc gtcgatctcc gacggtgcgg cagcgctggt gctcacccgc
    23041 aagagcattg ccgaggccaa ggggctggcg ccggtcgcca cgctgatctc ggtgggcgcc
    23101 catgcgcagg agccgcgcct gttcaccacc gcccctgtcg gcgccatcca gagcgtgctc
    23161 gacaaggccg gctggacggt tggcgatgtc gatctgttcg aggtgaacga ggcgtttgcc
    23221 tgcgtggcca tgttcgccat gcacgatctg ggcatcgccc atgacaagat caacgtgaac
    23281 ggcggcgcca cggcgctggg ccacccgatc ggcgcttcgg gcgcgcgcat catcaccacg
    23341 ctgatcgccg cgctgaaggc gcgcggcctc aagaagggcg tggcggcgct gtgcatcggt
    23401 ggcggcgaag gcacggccat cgccgtcgaa ctggcctgat aaaacacaag acgcgggcgg
    23461 gggcagcccc ctcgcccgcg ctgctttcgt ttacagggcg ccgctgcccc agatctgatc
    23521 ctcgcggatg aaaccgccct tgccggcgat gtcgatatgg caccagccct cgcggcaggg
    23581 gcccagcgcg ccgacaacgc ccggcgctgc ccgccacagc acggccgaac ccgccgccgc
    23641 cgccgcatgg atatccgccg aggggctggc ggtgacatag gcgccgcgct ccttggtgag
    23701 gaacttggcc agcacccagc cgcgcgcgcc atccgggtcc tcgatcagcc gccagccttc
    23761 catcacccgc agcactttca ccggcagatg cgcccggtga tacacccagt tgatccggta
    23821 atcctcaccc gggcccaccc gcatattcac ctcatccacc ctgagcgagg cccaataggg
    23881 cggattgctg ggcgctgtgg catgcgctgg tggagcgggc gccagcaaca aggctgcggg
    23941 cacaagacag gaaagcgcca gggcgagcgc tggaaggaag ggctgtgggc gtcgcattgc
    24001 cccctcatac cgcgaggcgc accgctgccg caaggccgct gcgcgcaggc atgcacaacc
    24061 atatgcccca gccgcttgac cccgccccac agcaggccca cacaggcccg atccaccctc
    24121 agggccctgc ccgcagatct gccgcagaag atgaccgaca ccgccagcca tcccgccgcc
    24181 gccccggacc gccgccttgc cacccggccg cgcgtgatcg ttacccgccg cctgctgccc
    24241 gagacgcaaa gccgcatggc cgagctgttc gacgtggagt tgaacgccga tgaccggccg
    24301 atgacccgcg ccgcgctggt ggacgccatg gcccgcggcc acgtgctggt gcccaccatt
    24361 gccgaccgca tcgatggcga gatgatcgcc gaggcctgcg ccagggccac cgatggcccg
    24421 cgcgtggggc tgatcgccaa tttcggcgcc ggcatcgaac atatcgacct ggccgccgcc
    24481 cgcgcccaca agatcatcgt caccaacacg cccggcgtct tcaccgaaga caccgccgac
    24541 atgacgatgg cgctgatcgt ctcggtggcg cggcggttcg ggccgggcat gcaggtgctg
    24601 cgcgaagggc actggggtgg ctgggcgccc tccaccttgc tgggccaccg catttcgggc
    24661 aagcgcctgg ccatcattgg cctgggccgc attggccagg ccgtggcgca ccgggcgcgc
    24721 ggttttggcc tgcaggtggc ctatcacaac cgccaccgcc tgcccaccgc tgtggaaaac
    24781 atgctggccg tgcaatatga acccgacctc gaccggctgg tggccgaggc ggacatcctc
    24841 tcgctgcact gcccggcagg ggcggccacg cggcacatcc tctcagccca gcggatcggc
    24901 gcgatgaagc cggaagccta tgtgatcaac accagccggg gcgagctgat cgacgaggat
    24961 gcgctgattg ccgcgctgga aagcggccgg ctgggcggcg ccgggctcga cgtgttcgag
    25021 catgagccgg cggtgagccc gcgcctgcag gccctgccca atgtggtgct gatgccgcat
    25081 atgggcagcg ccactttcga aggccggcag gccagcggcg agaaggtgat cgccaatatc
    25141 cgcttctggg ccgatggcca ccgcccgccc gaccaggtgc tggaaggctg ggcctgaagg
    25201 agctgggcct gaagagaaaa gggaggaccg caaagccctc cccctgatcc ctcaatcgcc
    25261 ggttttctca atcaccggtg agaatgcgat ccaccagctg cttcaccgtg ggggtgaagt
    25321 tgttcgaata gaacggatcc tgccggaagc gataggccgc gtggccggca aacatcaggt
    25381 tgcggtccac ctcgccgcca tgggcgatgt cctgcagggt cttctggatg cagaagctgc
    25441 gcggatcggc caggcgcccg gtcgaccagt cgtcatggtc cttccacgcc gagaagccgc
    25501 agtgcgacag gcagcccatg caatcggcct gatccttgcg gatttcctcg cgctcggcat
    25561 cggtaacgaa aaccaccgtg tcgtcgggcg tgcgcagcgc atgggcaaag ccctgcgcca
    25621 cccagccacg ggcgcgttcc agatcgcgcg gggccaccca gaagttcttg cccttcacgc
    25681 ccacgtcaag ctgcacggtg tgctcgcccg cttccacccg cgaatagggg atctggcgca
    25741 gcgaacgtgc ttccagcccc agcaggaagg ggttcttcac cgccgaggaa tagaagccgg
    25801 tggggctgaa cttgtgcagc agcacatcgc ccggctccag cgtgcgcagg tggtctttcc
    25861 aggcctgcgg gatcgggctt tcctcggtca gcagcgggcg ggtgccatac tggaaggcga
    25921 tggcgcccag ttcgggattg tcgatccagt gctcccactc gcgcagatac cacacgccgc
    25981 cggccatgat gatcggcaca tcgtccgaca caccctcggc gcgcatcgtc tcgcgcaggg
    26041 ccttcacgcg gggataggga tcctgcggct gggtcgggtc ctcggcgttg gaaaggccgt
    26101 tgtggccgcc agccagccac gggtcttcat agaccacggc ggccatcagc tcgctcacct
    26161 tgtgataggc gcgcttccac agggcgcgga aggcccggcc cgaagagacg atcggcaggt
    26221 agttgacgtt gaagcgcgcg gcaatttccg acagcttgta gggcatgccg gcgccgcagg
    26281 tgacccccgt caccagcccg cgggtctttt ccagcacgcc ttccagcacc gcctgggcac
    26341 cgcccatttc ccacagcacg ttgatgttga tggcgccctt gccgctggca atatcatagg
    26401 cgcggcgcac ctgttccacc gcgccatcga tggcatagcg gatcagttcc tcatgccgcc
    26461 cggcgcggga aagcgcgcga tagatctgcg ggatgatcgc gccgtcctcg tcgtagctgt
    26521 cggcgttgac ggccgacacc gtgccgatgc cgccggccgc cgcccaggcg cccgagctgg
    26581 cgtggttgct caccgccacg cccttgcccc cttcaaccag cggccacact tcccggccgc
    26641 catagaggat cggcttcaac cccttgaaag tcatcccagc aaatctttca caacaaaggc
    26701 ttaaccatcg catcttgcgc ccgcgaaggc atgttggcaa ggcccggcaa gcccggtggc
    26761 gatcatcgcc ccccgtttcc caagccgcca tgatacgcgt ttatcgcagg catgcgaata
    26821 aaaaccgcct aggcaagaac acttagcgcc acaaaaacgc cataatgctg cctcaatcga
    26881 cgtcttccac cgccaccttc tcgcccgtca ccttctgcga cagggccgcg gccatgaacg
    26941 gatcaaggtc gccatccagc acatccgacg gcgtcgggct ggtcacgccc gtgcgcagat
    27001 ccttcaccat ctgatagggt tgcagcacat aggagcggat ctggtgcccc cagccgatat
    27061 cggtcttggc ggcgtgttcg gcgctggcgg cttcctcgcg cttgcgcatt tcctcttcat
    27121 acatgcgcgc cttcagcatg ttcatcgcga tttcgcggtt cttgtgctgg ctgcggtcga
    27181 tctggctggc aacgatgatg ttggtgggaa tatgcgtgat gcgcaccgcc gaatcggtgg
    27241 tgttcacatg ctgcccgccc gcgcccgagg cgcgataggt gtcgatgcgc aggtctgccg
    27301 ggttgatctc gatgttgatg ttgtcatcga tcaccggata gacccacacg ctggaaaagg
    27361 acgtgtggcg gcgggccgaa ctgtcgtagg ggctgatgcg caccagccgg tgcacgccgc
    27421 tttccgtctt ggcatagcca taggcgttct cgcccttgat cagcagcgtg gcgctcttga
    27481 tgccggcctg ttcgccctcg tggtaatcca ccagctccac cctgaagccg cggcgctcgg
    27541 cccagcgggt gtacatgcgc tgcagcatct gcgcccagtc ctggctttcg gtgccgccgg
    27601 cgccggcatg cacttccaga taggtgtcgc agccatcggc ctcgccggcc agcagcgcct
    27661 gcaccttgtc ggcatcggcg cgggcggcaa gggcggccaa ggtggcaagg ccttcctcga
    27721 tggtggcgtc atcgccttcc atctcgccca gctcgatgaa ttcgatcgca tcgcgcattt
    27781 ccgcgccgat ctcgttcacc gtgccgatcg aggcttccag ccggcggcgt tccttcatca
    27841 ccgcttcggc ttcccggggg ttgtcccaga gcttggggtc ctcgacgcgc gcgttcagct
    27901 cgtcgaagcg gcgcagcgcg cgctcccagt caagaaactt gcggaccagc gcgagggcag
    27961 cttcgatccg gtcgatatgg gcctgccctt cggcacgcat gggcttaact ccgtcaaaca
    28021 aggcaagcgc cgcgcgcagc ttggcgacgg cgaaaacgcc cgcttagcga aagagccggt
    28081 tttgtaaagc cgccgccgtg gaaatcggcg cgcggcccgt caatgccgca gcgcccgcac
    28141 ggccgccagc gtgcgcgtta catgctcgtg ccagtccata tcggcatagg ccatcgccac
    28201 gcgcccctcg cgggtgatca cataggaggt gcggctggta aggcccgtat ccaccccgtc
    28261 gcgcttcagc gccacgtcat aggcggcgat caccggccgg ccggccaccg ccaccggaaa
    28321 ggccgagcgg cactcctcgg tggaaaagcg cttcagcgtg ggcagatcat cggccgacag
    28381 gcccagcagc gtggcgcccg ccctgtggaa atccttggcc gcctcggcaa aggcgtgggc
    28441 ctccagcgtg cagccctggg tgaaggcctt ggggtagaaa tagagcacca ccggcccgtg
    28501 cttcagcgcc tgggccaggt ggaaggagaa ggtgctgccg cccttggccc cttgtgtggc
    28561 aaaatcgggg gcggcggcgc ccacggccag ttcggcatgg gccgaaagcg gcgccagggc
    28621 ggccgcggcc agcgccagcg ggcggacaag cgtggaaaag cggatcatgc gtgccatctc
    28681 cttatcacgc cggttatcgc gccggccggc ccggccatgt cgtcatacgc cgatcagggc
    28741 ccggtgtcct gcgtcaggtc gccgggggcc tcttccttct gcaattcgcc gctctggccg
    28801 gtcaacacaa ggaagccctt gcggatctcg tcgagcagtt cctgccggcg ggccaccagc
    28861 tgctgctcac gcgtggggcg ctgcggggcg gtggccggct tgaaggcttc ccagatcacc
    28921 tcggccttga tgtcgtcaga aggcgtgcca tcgaacacgc gctggccggt cacccggtcc
    28981 acccgcacca tctggatgcc gggggcggcg atgaagggcg tgtggctcca gcggggccgg
    29041 gtatcggcga tgaactgctt catgatcggc gcggcaaagg tgccgccctg cacattgccg
    29101 cccagcggcc ggggctggtc atagcccatg tacacgccgc cgatcatcgt ctgctggccg
    29161 cccacgaacc acacgttggt ggggcccgag gtcgtgcccg tcttgccgaa cagcggcaag
    29221 ttgagatcgc gcagcgccgt gccggtaccg cggatgatca ccccttccag catgtgcacc
    29281 acctgatagg ccgtggtcgg ctccatcacc acatggccgc gctgggcgaa gcggggcatc
    29341 ggcttgccat cccattcgcc catgtcgcag ccttggcact tgcggctgtc ggcccgccag
    29401 atgacatggc cggcgcggtc ctggatatag tcgatcagcg actggtcgtg ctgcaggcca
    29461 ttgtctgcca gcgcggcata ggcgttcacc atctgcgcca cggtggtttc ccccgccccc
    29521 agcgcatagg agaaataggg cggataggtg ccgatgccca tgcgcttgaa ggtgcccacc
    29581 accttgtcca tgccggtatc gctggcgata tgcacggtca tcagattgcg cgattgttcg
    29641 aggccccagc gcatggtgtg gatgccgccg ccgccttcat cgccatagtt cttgaagcat
    29701 ttctgcccca gcgcggcgcc ctggaacacg cagaaggcct gatcgggcac catggtggcg
    29761 ggcgtcatgc cgttatcgag agccgtggca tagacgaagg gcttgatggt ggagccgggc
    29821 tggcgctggg cttgcgtcgc gcggttgaaa ctgtcgagcc cgaagtcgaa gccgccggcc
    29881 atggccagca cgcggccgct ttccggcgct tccaccagca tgccgccaga cacgccgggg
    29941 acgatgcgca cggcatacag ccccgcgttc accggcgctg cggcaaccac atcgcccacc
    30001 cgcacgccat cgggaatgcc ggtgagctgg gcggtgaagc catcgccgaa gccgatctgc
    30061 ccctgcccgc cgccgcgctg cagcaccagc cccacccgcc agttctgata ggacaccgat
    30121 ttgttgagcg cgttgagctg gccctgccaa ttgtcgcggt ccagctcgac atgggcgatg
    30181 gggccgtgcc agccccggcc cgccgcatag cgcagcaagc cggccgccag cgcgttctgc
    30241 gccgatttct gcagctccgg atcgagcgag gtgcgcaccc acaggccgcc ggcataaacg
    30301 ctgttggggc catcatcggc gccttcgcca tactggtcga gcaggcggcg gcgcacttcc
    30361 tcgacgaaat agccgacgga aggatcatag ctgtcgatat ggcggttgac gaggcccagc
    30421 ggctgggcct tggcattatc cgcctgggcc tggctgatat agccgttctt caccatctgc
    30481 tcgagcaccc agttgcgccg ggcgatcgcc ttgtcggcga atttggcgcg cgaataggtt
    30541 tccggcgcct tgggcagaat ggcgaggaag gccgcctcat gcagcgccag atcgccgaca
    30601 tccttgccga aataggcccg cgcggccgcc tgcacgccaa agctctgccg gcccagcggg
    30661 atctcgttca gatacagctc gaggatctgc tccttgccca gcacgctttc gatgcggcgg
    30721 gccagcagca tttccttcaa tttccggctg acggaatatt cgttgccgat gaggatgttc
    30781 ttggccacct gctgggtgat ggtggagccg ccccgggcgc gctcgccagt gccgaacttc
    30841 agcgcataat cgaccaccgc ctggcccagc ccgacaaaat cgacaccgcc gtggttgaag
    30901 aagttcttgt cctcggccga gaggaaggca tgcaccagct gcggcggaaa atcgacatag
    30961 cgcagctgca cccgccgctc acgcgcatag gaatagatga tgccgccatc agcgccgcgc
    31021 accatggtcg gcagcggcgg ctgataggac accagcttgt cggccgaagg caggttgcgc
    31081 accaccagcc cccacaccag cgcataggcc agcgccatcg ccagcagcgc gtaattggcc
    31141 aggcggaacg gccggctgtg cgcccagcgc cttgcatacc agcgccccgc gccagtggca
    31201 ttcatccgcg ccagcaagcc ggaggcattg cgccggatgc gatagctcag ggtctcgtcg
    31261 ggttcgcgct cggtcatggc cggcgggccc tagcatggcg gggggcgcct tcgccagagg
    31321 agatcgcctt tcgcacgccc gctctcgcgc cggcctattg cggcccggcc agccctgccc
    31381 gctcggcaaa ataggcctgc atggcgcggg ccaacgcatc ggcaaaggcc tggcgccccg
    31441 catcggaggc caggcgcgcg gcatcctcgg cattgctgat atagcccgcc tcgaccagca
    31501 cggagggcac atcgggcgtt ttcagcacca cgaaagcggc cgattgcacc ggccttgccc
    31561 gaaagcccag gcgcccggcg ccggcggcca gcacatcacg cgcaaaggcc gccgacagcg
    31621 ccgcgctgcg ccgctgcgac agatcaacca gcagcgcgcc ggcgctggcg ctggcgctgc
    31681 gcaggggcac gccgctcacc cggtccgccc cgttttcacg cgcggcgatc ttttcggcct
    31741 cctcgctgct gcccctggcc gacagggtgt agacggtggc accgcgggca tcggccgcat
    31801 cggcgctgtc ggcgtggatc gagacgaaga ggtcggcgcc cagttgccgg gcaatgccgc
    31861 tgcgctcctc cagcgccaga tagcggtcgt cggcgcgggt gagcgccacg cggaaatggc
    31921 cggtggccag cagcctttcg gccagcgcgc gggccagcgc cagggtcagc gccttttcct
    31981 gcaaggggcc attgccggcg cccggatcat gcccgccatg gccggcatcc agcaccacca
    32041 gcggcaggcg cgcatccatg ggccctgcca cgcgcggcag gcccagcgtg ccgccggccg
    32101 gcgccagttg caggcgcacc acataatcgc gccgcagaac accgctgccg atcacctggt
    32161 tggccgtgag caccagggcc agcagcgcca ccggcagcag aacgacaatg gcaatcgaga
    32221 gaacacgcgg catcggcgaa caaggccctt ggcaagggag gcatatggca gccccaacgc
    32281 gcagcgggcc gcaagcgcca tggttaatcg cgccgggcgg cgggcgcaat ccttgccgcg
    32341 cgcggcggtg cttcagcgac gcgcccggcg ctgcttcagg gaaacgtgca agaaacttgc
    32401 cggatgggcg cgcggatgct aagggcattg ttcccggagt gggtgcggca aggatcggca
    32461 tgcacgcgcg ttggccagcc atggcccggc gcggcaacag gccttcagat cgcccgctgc
    32521 ccaccccgga tgcccggcgc cattttgccc ggagctgaca ggttgatgca agttatgaaa
    32581 agccgtttgt tgtttgcgtt gccgaagccc ctgccggggc cggctgcgtg gatggcacac
    32641 ggcgcgcaat gcggccttct gcgggctgca ggcgctctgg cgcagcagcg cgctcgccgt
    32701 tccacagggg tggccgggcc gcaatcgaag cgcatttcca aacaaatccc gagaaccatc
    32761 gggtcgcccc gcttcggctg gcttgccaac atgcctatcg cccatccggc cctgtgccgg
    32821 atcaacgcca ttccggcccc gtgccggctt ctcgtccctg acgagaccct caacgtcacc
    32881 cccgtcgcgc ctgagcggaa gctgctgaag cttcgctgcg gcgcgcaacc gtttaccgcc
    32941 aattatcgcg ccctgcggcc ggccgccccg gcaccggcaa cgccttgccc ggcccaaccg
    33001 ccgcaggccc gcgatcggag atatttgaat gacaacgcgt atgctgatcg acgcacgcca
    33061 cccggaagaa acccgggtgg ccgtgctcaa gggcaaccgg attgaagaat tcgatttcga
    33121 ttccgccgaa cacaagcaga tcaagggcaa catctacctt gccaaggtga cccgtgtgga
    33181 gccctcgctc caggcggcgt ttgtggattt tggcggcaat cgccacggtt tcctggcctt
    33241 ctcggaaatc cacccggatt actaccagat ccccaaggaa gaccgcgagg cgctgctggc
    33301 cgaagaggcc gcccatgccg aggaagaagc ggccctgcgc gcagtggccg agggcgagga
    33361 ggatttcctg ggcgatgacg cgctgggcga aggcctggcc gatgatctgg cgggcggctc
    33421 ggtggaagat gccggcacgc tgatcgaggt ggacacctcg gagaaggatt cggtcgccac
    33481 gatcgaggcg ggcctggtgg aagagggctt cgatgaaagc cacgccgccg aggaagccgc
    33541 cgaggacgag atcggcgccg atggccgccc gcgccgtggc cgccgccagg gccgcgcgca
    33601 atccaagcag gccgacgaga tccgcgccaa gcgcatggcc ctgcgccgcc gctacaagat
    33661 ccaggacgtg atccaccgcc ggcaggtgct gctggtgcag gtggtgaagg aagagcgcgg
    33721 caacaagggc gcggcgctga cctcctatct gtcgctggcc ggccgctatt gcgtgctgat
    33781 gcccaattcc agccatggcg gcggcatttc gcgcaagatt tccagcgttg ccgaccgcaa
    33841 gcgcctgaag agcatcatcg ccgagatgga cctgcccaag agcatgggct gcatcgtgcg
    33901 caccgccggc cttcagcgca ccaagaccga gatcaagcgc gacttcgact atctggcccg
    33961 cctgtgggac gatatccgcg agacgacgct gaagagcagc gccccggcgc tgatccattc
    34021 ggacagcgac ctcatcaagc gcgccatccg cgacatctac aaccgcgaga tcgaggaagt
    34081 cgtcgtcgag ggcgatgatg gctatcgcga cgcgcgcgac ttcatgaagc tgctgatgcc
    34141 cagccatgcc cgccgggtga agcattatgc cgatccggtg ccgctgttcc agcgctatgg
    34201 cgcagaagac cagctttcgg cgatgtatga cccggtcgtg cagctgaaga gcggcggcta
    34261 tatcgtcatc aacccgaccg aggcgctggt ctcgatcgac atcaactcgg gccgctccac
    34321 caaggagcat ggcatcgagc agacggcggt tgccaccaac ctggaagccg cgcgcgagat
    34381 ctcgcgccag ctgcgcctgc gcgacatggc cggcctggtg gtgatcgact tcatcgacat
    34441 ggaatatggc tcgaacgtcc gcaaggtcga gaaggccatg aaggaagcgc tgaagaacga
    34501 ccgcgcgcgc atccaggtgg gccgcatctc gtccttcggc ctgatggaaa tgagccgcca
    34561 gcgcctgcgc accggcgtgc tggaagccac cacccgcgcc tgcccgcatt gcgatggttc
    34621 gggcctggtg cgcacggcag gctcggccgg cctttcggcc ctgcgcctga tcgaggatga
    34681 ggccgccaag ggcaagggca tcatcgtctc gctgttcgcc agccaggaag cggcgatcta
    34741 tgtgctcaac accaagcgcg ccgatctcgc cgagatcgag gagcgctatg gcgtcaacgt
    34801 cgaggtgatc cccgagggcg agaatgaggg cgccaagatg cgcgtcgtct cctctggccc
    34861 gcgcaacgaa ttcgtgccgc ggttcgaggc gattgccttc gaggaagagg aagacgattt
    34921 cgtcgaggag gaagaggacg agatcgagga ggccgaaggc ggcagccgcg gcgatgatgg
    34981 cgaaggcgac ggcaatggcc gccgcaagcg ccgcaagcgc cgccggggcc ggggccgcga
    35041 ccgccgtgag gaagaggctg aggacggcgc cgatgagggc gaggccgcgg gcgacgaggc
    35101 cgaagccgac gaggcggtgg acggcgaagc tgctgacggc gagaccggcg aggctgtgga
    35161 cggcggcgag gaaggcgatg agacgccgcg caagcgccgt cgtcgcggtc ggcgccggcg
    35221 gggcgggcgt gagcgccgcg aaggtgcgga aggcgccgag gacggcgtga acgaggacgc
    35281 cggtgacgaa gccgaggccg ccgaggccga agcggaagcc ccg
//
LOCUS       DASRYE010000046        31533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_143960,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000046 DASRYE010000000
VERSION     DASRYE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 31533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31533
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143960"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFF98_02715"
     CDS             <1..1314
                     /locus_tag="VFF98_02715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZU62569.1"
                     /translation="PAPATAPAAPPAPAAAAPVAAAAPAAHAPAAAANAAGAPPAAPA
                     PGQSIRIDLVKLDKLIDTVGELVIAQAMLAQRLQNEGFQSTEELTLLEGLTRDIQENA
                     MSIRAQPIGSVFSRVPRILRELAASTGKHVNLQVFGETTELDKTVIERLGEPLTHLIR
                     NAVDHGIEKPEDRLAKGKSAEGVLTLSAEHRSGRILIKIADDGAGINRARVFAKAVEK
                     GIIGPDAQLSKEEVEALIFAPGFSTAATITNVSGRGVGMDVVRQNVKDLGGRITIESE
                     EDKGTTFILTLPLTLAISDGMIVKVGDQTLVVPLTHVVESLRPDARDVQGLGSQRQML
                     NVRGRFIPVVSVAGSVGARGAVACPQDGVLIVVDTESAGQAALLVDNIWDQRQFVIKS
                     LDTHYRSVDGVAGATILGDGRVALILNVDTLVASAVAAGLGLEAA"
     gene            1311..2207
                     /locus_tag="VFF98_02720"
     CDS             1311..2207
                     /locus_tag="VFF98_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HZU62570.1"
                     /translation="MNAAPPLAAPPLAGAFAKDMPGISPDVYGEADFRAVAQIVREEA
                     GIMLPPGKAMLVYSRLAPLVRASGKGTFKAFIEMIRERPEERRKAVCALTTNHTFFYR
                     EQHHFEHFAHTVRPWLLDELGHGEAVRMWSAGCSSGEEVFSLTMSLLGPDRPAGKALA
                     SRDVRILASDIADHALAKASAATYAEQDIAALPAPLREAWTTSTAGQVTIGPEARGLV
                     QFKRLNLHGEWPMRRPFQVIFCRNVMIYFDQPTKDRLVERFAHQLVPGGFLYIGHSER
                     VSGAAESMLDTVGPTIYRRRMR"
     gene            2204..3244
                     /locus_tag="VFF98_02725"
     CDS             2204..3244
                     /locus_tag="VFF98_02725"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HZU62571.1"
                     /translation="MTIRVLIVDDSATMRAVLAARLSGFPDIQVVGLSCNAAEARAAI
                     KELAPDVVTLDIEMPGMNGLDFLHKIMTLRPTPVIIVSGLTQAGSDVTARALALGALD
                     CYCKTDYAGSAMQDGGKLAEMVRTAAQVNVHRARPVMPAHDAQASALARTGTSGDTKV
                     IAIGASTGGVEALQVLLSGFPADCPPTLIVQHVDARFAPSIARTLDASSPATVCLAEP
                     DMPITRGHIYLAPGDDRHMLLAGTSRLHVKLRNGEPITGHRPSVDALFASVAQRLGAQ
                     AVGILLTGMGADGARGLLAMHQAGAFTIAQDEASCTVFGMPRVAISMGAASLVAPLGR
                     IAQHAFSGKVAA"
     gene            3244..3798
                     /locus_tag="VFF98_02730"
     CDS             3244..3798
                     /locus_tag="VFF98_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HZU62572.1"
                     /translation="MPFDQKQPGPPITRINILQGQARVSAGPRVEFSTVLGSCVATCL
                     FDPEVEVGGMNHFLLAEPPAGAATGDFDEHYGVYLMELLINEMLAHGATKHRLRAHLY
                     GGANLNSGFARIGTRNAEFAHHFLAKERITVVREDLGGIHARRVDFRPARGQVRCRTV
                     ENTLAPEPRPMPRPQKARGDVELF"
     gene            3817..4188
                     /locus_tag="VFF98_02735"
     CDS             3817..4188
                     /locus_tag="VFF98_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU62573.1"
                     /translation="MNKNTRILTVDDSASMRALLNHALTSQGFDVAQAEDGEVALEWL
                     ALNEVDVVITDINMPRLDGFGLIERLRQGNRHRDRPILVLTTESSEEKKTRARQAGAT
                     GWIVKPFDPEKLVAAVRRVAH"
     gene            4211..4651
                     /locus_tag="VFF98_02740"
     CDS             4211..4651
                     /locus_tag="VFF98_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZU62574.1"
                     /translation="MHRELITFEVGGQVFGLDIMAIREIRAWTPTTRLPHVPYYVAGV
                     VNLRGTVLPVIDLAARLGWEATEPTPRHAIIVTQLGAKACGLIVDSVSDIVTISQDQL
                     QPPPVSANDSITHFLEGLAAIDERMVMVLNLAALGDGDQFGEAA"
     gene            4648..4923
                     /locus_tag="VFF98_02745"
     CDS             4648..4923
                     /locus_tag="VFF98_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62575.1"
                     /translation="MMQDAHIAAHLTTVSDILRELAGEIEELGTVLCLEPSLVQRHMG
                     TLQAIDLIAQKQRWLATLLKADCPLSAVSTIGVEALRLRFSPSSGPN"
     gene            complement(5110..5595)
                     /locus_tag="VFF98_02750"
     CDS             complement(5110..5595)
                     /locus_tag="VFF98_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62576.1"
                     /translation="MDKPTVLTMQPGEWRYRLAELCRDCALADAGEQADMLRMARSLF
                     AIAPEDASPADAWPVPGLDEFERLLMVGAWESAAFDLMPPEATCLVSRSGEDSCMASV
                     MLPGLAEEVSSDGDTVAMALTSALAAAISMLNAEDGRVVGLYHEERLSPGVRRAPSAL
                     H"
     gene            complement(5820..6500)
                     /locus_tag="VFF98_02755"
     CDS             complement(5820..6500)
                     /locus_tag="VFF98_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZU62577.1"
                     /translation="MPALIWAVPALAGPPYVTDDPEPTDLHKWEIYNFVSGSHENGIT
                     SADMGVDLNYGGAKDLQLTATLPLHVETGSPLGAGDVELAAKYRFLHQQEGGLLPDVA
                     FFPRVFLPTGRGSTRAQVLLPLWAQKDWGKWSLFGGGGYTLNPGPGQRNYASMGAALS
                     RQLRPGWQMGLEYYAQTRASEDDRPVHGLNIGSQVHIAGPFSWLASFGQGLNRRQTIF
                     YSSLKLDL"
     gene            complement(6681..7685)
                     /locus_tag="VFF98_02760"
     CDS             complement(6681..7685)
                     /locus_tag="VFF98_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU62578.1"
                     /translation="MGKIRNIKELADLAGVSTGTVSRALAGSELISLKTRERIQALAR
                     EHDFRPNVLARNLRIQRTQTIGVLIPLGHEAGQHMSDPFFITMLGLLADALTERGYDL
                     LLSRVVPADDGWLARMTDSGRVDGVIVIGQSDQAGTIDRVAARYRPLVVWGGYRPGQV
                     HCSVGVDNRLGGDLAAGHLIARGCRRIAFLGDARALEIEQRLLGVRDALARAGLEGGL
                     QVLPTALVAQTAREGIGAFLAQASEMPDGIVAASDVIAMSALSALAEQGRAVPGAVRV
                     IGFDGLPLGEQTVPPLSSVRQDIAAGAGHLVSMLLRRIAGEETASVVMTPELVLRGSS
                     "
     gene            7946..8818
                     /locus_tag="VFF98_02765"
     CDS             7946..8818
                     /locus_tag="VFF98_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZU62579.1"
                     /translation="MTDTTQVTSQPASTITADPAPVGERELGAFAKLNAGNAGDFIER
                     LDRMQQYDGFRLYKEASFAAMRLAPGMVVADIGCGAGDDVLKLSHLVGPEGMAIGVDI
                     SADMVEEARKRFAGAPALDFTVAQADSLPFADGSLDAIRADRVLIHVPDPAAAIAEML
                     RVLRPGGRLVLCDPDMVGFWVSSADPMTSQIVSGAIAKSCVNPFLARDMGVMLRDLAL
                     PEVAHHAMTMVTDDFAVVDRVVRFELVAQKIAAAGLLPAGQIEGWLAEQLQRRDEGRF
                     AAGLSVMMACATKG"
     gene            8885..9919
                     /locus_tag="VFF98_02770"
     CDS             8885..9919
                     /locus_tag="VFF98_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU62580.1"
                     /translation="MVTAPVTILADTARVRPADVLAYALALPRLAPLRVPDLPYTALA
                     RLSALAPGALPPLHSFIASDGARLAYRSYGPRAGLQLLLIHGSACFADQLHHLASHIA
                     SQGLAAVHTIDMRGHGQNPRDQHAPANRFAADVAEFALALKRQTPLNRIVIGGHSAGG
                     GLVTQVARLPMARHIAGWLLLAPYLGVATGTVRPFFGGWLTGIDRAGLARALLASLAG
                     RPTGQRPLARFNREAVLHDPRFVRTWGFETVFGFGPGSVAGTGQPYIPASAPVLLLAG
                     DADNCFLPAAYPAALARIAPHGEAVILPGLGHWDILAQPEALAATAGWLQRHWSKGHA
                     FKKEEVANAN"
     gene            9909..10580
                     /locus_tag="VFF98_02775"
     CDS             9909..10580
                     /locus_tag="VFF98_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZU62581.1"
                     /translation="MPIDICKPLPRWGALAALLLPLPALAADDSGWHRGDFNLRLGAA
                     GVMFGESGRVSVAGSPVAGSNITLTDNTTLAGEIEYFPTAHLSVAVNFGIPPQTTVGG
                     AGTLAPLGQLGKVSYGIGAAMLRYHLFAHSRISPFAGVGVGHFFPTATHDGAVTGLRV
                     GTATNVALQGGLDVHVNRHLGVYADVSYAPLKTTATGFILGFPALADVTLNPTIVQGG
                     IAYRF"
     gene            11099..27601
                     /locus_tag="VFF98_02780"
     CDS             11099..27601
                     /locus_tag="VFF98_02780"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZU62582.1"
                     /translation="MTIVLRIASHETGRHVTKKLHKGMNDIVLPAGAHVEVADGDGQA
                     VLRPVKAEFVQGKLILDYAQEDGTVQLRVAGAALEGLDSAGPLELAALHTAFDSTVVA
                     GTDWAGAGWADADWSGADGAGSAGAGGNEDTDDSGHHHHGLSTTLLLLLGLGAAGGVA
                     GAVAGGGGHSGSGGSSATAPATPVLALGTGIGSNGANATEATQASGVVTVNAASGSSV
                     DVTFTNGSHTVTKTVSGTGSAVAVVLTQADLATLGDGTISVSAVAKANGLSSSAASDN
                     FLLDTTAPAAPVLALASDTGSSATDHITSNGSVNLTAESGSSVAITFTNGSHTLTKTV
                     TGTGSAQTITLSSADAATLGDGAISVAAVATDAAGNASTAGTLAYTLETATTVAVTSQ
                     PILVGGNPVLSGTAAAGDTIVLTIAGATYQTTATGGIWSVDTGSAVPVSGTLALNANG
                     TNAVSVQATDAAGNTASASQSLQLVSASSGVVNTDATTVAVQVNAINANVPITINAPN
                     ATSLTITGNTNGSEVDLDIPDKTPGSVDTYPLTLVTNVTASSNPAQPNLLVIDFASAQ
                     DRVVLSSASSIKGFNQIEVRNGTLDLSGVNITGIVDIQINSGVVLTLAQFESLSSLVS
                     LSGLGQLTIHLDTQSQINDLISFLQSPGNLFLMGTTVTVEYGSSNTVISNSTAASLIS
                     AMDYPSIPAIASAISALQSAVSGNTTSIALLISDASTHNGTPTTASIAGLQAALAALQ
                     SEVAQQQLDLEAIQAHNLDSLQTLVTNLSNTVTALTAQVNAQASEIAANTSDIATQAN
                     QIASLLTSVGALQTTVSALQTGNSSNAAAISALQTAQASLAATVSALQTTLQNDIASL
                     EGTNYANATYKDIASLSAQIVALNSAVAALQGLVGQSSVAAQISAAIGHPTNSSGAAT
                     GLWVSIESAISAAAASLQSEINSTNANLAQVIDDSSTHGGTPTLNSIAGLLAAVNVLN
                     GSGAGSVSAQITAAIGHPTNSSGAATGLWAGIESAINAEAATLNAEIAALQGANFNSA
                     TYQDIASISAAITGLQALVGNSSVSSQIAAAINGVTSSFDTLISALHGAGVVLTVAET
                     AALLVSDPAQLLTNYRLVDSYANLLAATQNAADAHVFAYAKTIGFTATSALSVAQVSG
                     LESAIAAIANAAPVSAALSSYSLADTVADLAAGSPAILSGATNITATLGSASLAQVAS
                     VLAATNAGTTVLDAVAGTPAQILALPISAHDTITAITVTGLATAQQAADLEQLASAGR
                     AGSVQLGAVADTAANIEAVIASQNGATFLASASRIDVSGTLSVAEAEQLQAVNANVHI
                     TALADSAASITSALAASQLSLTYVTALSTTDTATIAEASALQAALPGHVSILGITDTS
                     ADIGAAVVAGTLSLTGVGAVTIADGSTADYASAAQGAALGRSSLALAYDVADTSAHYA
                     AAIATYGQGFLAKADNIVLTGAASIAVARSLLGAQNSGTVTIDTINATAAQVTSLNFD
                     AHDIVSHLNVAGLTYGDQTVDLTNVPFSTNLTYNGAALASSYSLLLYLPQPDGVNVDE
                     NGVDQAGEVVIHAGSGNNTFVFTDDLADGTYLPTAYASGSSIDYATYGAGQHGTETLV
                     VIGSVDISNITLNGDINSYDLEALAGSPSQVTLSARQLNMLHNFTGASGGTVINITNA
                     PDPATGTVVVTSLSNNALTQNINFTNVTGVNIGINDNLQIDFLRLLGLSHVTTASGSG
                     SSQAGLVTPVFNTAGGQTYLTTDGTQNGTPISPSFLTNASYNIDMSAAAGLSLGNDGA
                     GHYYLQDSQGNELNLPQTLNGSTLALSPDEISGPGAPLVMGATNGGVLQLTGLVNSAG
                     HAVDVTQVSAPITLSIGQSTDFTSASNPAQITADLAHIAATAGSRITVNNVGGGTPPV
                     TLTLSADQASGLAIAGTGEVKVTGTATGNEDYSALSATTIDLSGVVPNGHSITLPASV
                     PAGHTLILNQAEVNGATIGGSGALNILLNSAAAMDLSHIDTSHLAVSLVVPTGTTATV
                     PSGSNLANFPIIVQSGATLNIAGDVASGKAISGAGTVNVTGTGTVAGGDDFSQITAHT
                     VDLSGYSGAIAHLPALSTSGVTALVLNTAEADFAASAPANALTVPSGTGITVVANGPA
                     SAPGTTLDLSGIHTTGTGTLAVHVTGDTVLASANLNGAPVSVDSNVTLTMPVGAAGGL
                     SISGAGTVDLTGGGAVSADLSHLTTTQVNWDVSENTTFTGTLGTAVVTIDHDVTLSGS
                     AARFAGAQVLAGAVDTGLGQSRGVLSITGAFTNQDFSGVAASLDLSHATSGTINNLTL
                     PTWVAGQALILSYLQGDGLVLDVGAGDARIVNIVADAASDLSGVTSTSGHVTAIIDGT
                     ATFTGTFSSAANASIHFVTGTGGATYTLSLDGHLLDGRTVDGSGTVNVTSLSASTDFS
                     LVAPTILDFSHDGALAVVSGQLSENGAIATLPSLTAGHTLVVTAAEANTLAVSGSGGT
                     VDVSGDVPASTDLTGIAAGIAISFADSTGAQAIIADGQQLTMASDQLTGQTIKSDTAH
                     SASGTLIVQGRGGSGTALTSAADLSAVSANIDLTGLANLVVAGGLLEDATTAAYALTL
                     PALAASQTLLVDAGQLGGDALALGGTGTIDVEGDITTVSVNLSGLGDGVHLSLQDSGG
                     NAIDIGSNLSLTVKAVQASGQAITGAGSLVVVDDTTHAAALVADLSQVTSTIDLRQFA
                     GLHVDGGAALDQLVDATGTLALALPVLASGQVLLLSTAELAGGLPLQVLGTGVVEIIG
                     TLDNQSINLTGIAAGNTVDLDSGALTMNHGAQLTATAGQLSGKGITALDSASTVVVAD
                     AATTPVLTAAAISAALAANPNAAILGNLVDTAANLATLDWTDNAFLFSVHGTVTATTA
                     ATLAETAVLYAINFYRAGMASFSVADTLANFAAAPQWLVEQAVERASVNGGTITVTDP
                     VSLADASALVADNLLFAANNQNQVDGQNWNGITFTVADSAAALAALGAGAAANLQAAL
                     QFGGGSITITGTATAAQASSLAAAYGSLVHYALADSGAALVARHTTDGNWTFLADASA
                     TEVTGSTSAANAITLEGAGALTIDLVTDSAAAIATAIGGHAHFLDSIAGISLTSAVAV
                     STLGSAAAQSVNYVSDTVSNLLGMGISAYTRLTAGAVISATGTANASQAQTLIQNFYN
                     DLNGGGNYPNATTEKSRLLLSIADTAANITALGVADLARLSGAVHVTNAASATQGVEL
                     AWLVANSGAHVYYSVADTAANLTGELTGAMAAYVTGTITATTTATAAEAYALYGLATA
                     SGVSAPVVFTLADSVANIEAAITAHGAGFLAQPGTIVVTDAVSVAEASAILAAAGQNA
                     TVTFTALTDTAADVNGASAGLLAHVSGTITKVLSAASTDLTGVSANIDLRGLATQGVA
                     LDAGTSRATIVINQLVASSGAVATREAYDVLLPTLAATQTLTVDAAELAGSLAAAIAG
                     SGQLVIAGDIAADTDLTGVAAAIGVSFKDSGESGAFGTVALAASAHLTAYDGQISAHA
                     ITGAGTLVVLGGASGGHLSGALDLSTVSASIDLTQALLSVDTAAGAHQGQLVDNGSHV
                     TLPTLTGQTVSLTIAELTGELAISGTGTLDLQGNITSSLDLTYLPATIALSFLDAGDA
                     GGLATVDVSGTAQSQTVLTLTADQASDQFITGNAYATIVLPYAAGAATNLSASTDLAH
                     VFSTDYATNAAWASALQAANAGLAGQIAATTNGVVLTGGNSDGAFETFQFTAQATGTY
                     SFTANFQNNYHGAYSYTDAYGTHIYYPDSDRYYLTTNVNGGGNQVYGWTSASGSTTYT
                     VTMVAGETLSIYLYTPGGRTAGADGALTTPSTLTLSNGGFSAVGGTAAALDVTVGQNT
                     DLTGSADPLAYVSVDQFTLANQAVLTMSGLEASGHLVTGSGSVVIAGNITSGWVDLTQ
                     VAVPLSFTNSVSDEQQLGINGDNWLRLTAAEANGLFTSGGTVNIGGDIADGASALIDL
                     THIQSTLSFEDTATTSRNVGAYTALTTTTTIARNELNTLSYATGWNYGQIYVGSGDGL
                     VLRADQANWHSVSGPGTTYVVGNLDDGDLSLLGITSRLSFWDGIVANPYGGDASEDPQ
                     STGKGSITLSEQSTMEVIGNQLDGNYILGDGSNVVRVYGALGDASHAIDLSHITTSID
                     LAYAYDHNVTLVTGLDGQGNITTNNGSITVGFNALTGGQKTVIAGSISGSYNTLSAYL
                     PENWNPDQTLTIYSRDANDLLLDTRDYSNGSLVLDESGNAVGAKVLIAHVEDLLSNGQ
                     INGDFSEINASLVTLNFGSRQGNVLSYAGSFGSATVLIDDGVNVVMDGAVASGVYFDT
                     QYSGYRWNGSGFGVVGINTLTANADLTNTVNDPASFNLTLPNIYGHVDIDLSYGAAGS
                     AYLPNGATAITWNAALGQFTNGTYAITLPSFDGENGLIVNASQLSGGTGFTLAGSGFV
                     GVEGNIAANLDLTQLSTSFAVYFGSNTTNIRNLAHGQSYPTGWDNWSAYSGTAVNSGV
                     TLTLRPDQIYNNIAISGAGTLALAAGTSAYADEFGNVDLISVTANIDLTGLGTQASSG
                     GISQDGTRDGTVINYREGNSWLNYTAGYTLGNGGYELLLPTLGASQSLTVLADQLAGA
                     NGFNLGGASGALLDIQGSVNSHVSLLNVTTGVTVSFRDGSETGLGAITVNAAGQLLAR
                     VDQLDGHSINGAGTVYAGITGTVPQISTADASALQEMASGHTADLTGITANLDLTALS
                     GTNTLAGLVINGSGQFADGTDATKLVNLPTLIAGQTLTVTALEMKGQLALVEAAQNGG
                     GTIDIQGDISDATGTVDLTHVEDGIAVSFADNANANVMLSGTSAQLIIKAGQASGLTI
                     TGDGSSAAAYNNAGTSASHFTGAAVVLADNDTGTLSTLATANSATVDLTGISADLDLT
                     QLTGLGVDANGALSDGYGKLVLGTSGAAAKPLRAQQTLKVNTAQLASLSAGGTGRVVL
                     NGQAGSIVDVRGNLASGVAIDLTGILGSTISFVDTQDVSANAITVAGTLTMLAEQFDA
                     AGGMKISGTGALWLEATQATTTVNWTDSAQTPAYYISNTSLASTLDVQDSTTLVAAGS
                     GLMPTAMQIKVEASQTLTSGYAYVSGRTIYDAAHQYSYNAQTGTYTETGGTLVVTGSS
                     VSALDLSHVTIGTLDLTQYGGTLFTGLWTDLSDHVLLMNDTQASGHAFTASGDGGALT
                     VMATTPATYHDFSGIGASVNSLLVVGGAADMADAISGANHTLLNVFSHVELNYASGLL
                     TVSADQVNGMTANRFTGTGGLYVRDDAATYHSGAETLYGSNNNDTFAGGGYGDTVDLT
                     GTGNTGGGTDTVLFSTLAGSSGHTVDFSIAGFTTGAGTGHDVLNFSALSAHTAHSGTG
                     DVSAVSLAAVPALLGQEVIVVTDSFGADASGVASVFGAGSNLLNKALAAAGNTDAIFV
                     VALADPNNSGSGAPSVNFGVWHWQEASGGNGYVHAAELTEIGVLHSLNQTQIAAMTSA
                     NVIG"
     gene            27952..29346
                     /locus_tag="VFF98_02785"
     CDS             27952..29346
                     /locus_tag="VFF98_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU62583.1"
                     /translation="MGAQTEGQAPAQAPAAGQALFGPPAPAGTPDGGEDGLDPAASGL
                     PTAEDMQPAAPPQPQPMPLPEAAPPLHTAPAAGFNPELAGLIEEAVHRHPSIASQRAA
                     LRAAGVDVTAAWLTRLPTMSIQFNQYGAIALSRQVTANVDLPLWSNGKIPATIDRARA
                     SRQAQFYRLAETVLDLELEVNQYYHESKRLAEHEQVLLRILAVMDDMVASMRRRVAQE
                     VSPLADLQLASSRRLQVAQQLDLVRAQKASAQAHLAELVLREEVPLAPGFVAPPSWPQ
                     WQLPALTERMITASPQRRRVQAEAQVARDDARITAASRLPGLFASYSYDEVYKHRVGL
                     SVRMQTAGGFSEIFSTRAARLRERASDLQAPAVEQDMKATASNDLVEYDSARARDAGA
                     RELADDSRKITESYVRQFVGGRRTWLDVMNAVREAMSADLDAIDVRYTAAAAAMRILL
                     RSGDTKTIVEGRPK"
     gene            29343..31448
                     /locus_tag="VFF98_02790"
     CDS             29343..31448
                     /locus_tag="VFF98_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62584.1"
                     /translation="MTLPLLDLVAALARRRGARLAPDWQAAVAVDLALDDGEAPWRLS
                     ELAGWEAPVVLAARPKPQDFPLLVYVEALGWALAEQWQGPDHIRAITASGLAEVEWGP
                     GLRLVQLSFPEAAGTGEYPSAFAVFRAAILGRRRMIVDATAATVVINVLALITSIYSM
                     QVYDRVIPRASYDTLWVLTAGMGLALLIDFLIRNTRAFLIDEEASEIDAEVSEYFFAR
                     MQAVRLDARPPGVGTMAGQLRGLEQVRAVMASASLFILADLPFALLFILVMYVIGGNV
                     ALVPLLTFPLAIGAALLFSRMIREDTHKSQVSGNRKNGLLVEALGAAETIKANLGGWH
                     MLAGWNRLVETVHRHDNRVRRWSTLSGSTFNVLQQVAYVGIIVVGVFEIADRQLTMGG
                     LIACTIISGRINGPLVAALPNLIVQWSYARSSLQALDNILRLPSDHPHDRQMLRLAEV
                     RTGLRLANVQFAYPGARSGLEIPGLFINRGEKVGVIGSVGSGKSTLLKVMAGLYAPAL
                     GQVTLDGVEVAQIAEDDLRRRLVCLPQSFELLSGSLRDNLALGIANPNDERLLAAAQR
                     IGLAELVRQHPMGLDLPIAEGGLGLSGGQRALVGLTRLLLTDPAMLLLDEPTANLDPE
                     TELRAMQQLFGHFADRTVVMVTHKPQLLTFVERVLVVVDGRIALDGKTADVLNQLRPK
                     PAPASGREVPDHRPTQEKD"
BASE COUNT         5313 a        11367 c         9959 g         4894 t
ORIGIN      
        1 cccgcccccg ccacagcgcc ggccgcaccg cctgcgccag cggccgcagc gcccgtggcc
       61 gcggctgccc ctgccgcgca tgcccctgct gctgctgcca atgccgccgg tgcgccgccg
      121 gccgcgcccg cccccggcca gtccatccgc atcgatctcg tcaagctcga caagctgatc
      181 gacacggtgg gcgagctggt gatcgcccag gccatgctgg cccagcgcct gcagaacgag
      241 ggcttccagt ccaccgagga gctgaccctg ctggaggggc tgacgcgcga catccaggaa
      301 aacgcgatgt cgatccgcgc ccagcccatc ggcagcgtgt tcagccgcgt gccgcgcatc
      361 ctgcgcgagc tggcggccag caccggcaag catgtcaacc tgcaggtttt cggcgagacg
      421 accgagctcg acaagacggt gatcgagcgc ctgggcgagc cgttgaccca ccttatccgc
      481 aacgcggtcg atcatggcat agaaaagccg gaagaccgcc tcgccaaggg caagagcgcc
      541 gaaggcgtgc tcaccctctc ggccgagcac cgttcgggtc gcattctcat caagatcgcc
      601 gatgatggcg ccggcatcaa ccgcgcgcgg gtgtttgcca aggcggtgga aaagggcatc
      661 atcggccccg atgcccagct ctcgaaggag gaagtggagg cgctgatctt cgcccccggc
      721 ttctccaccg cggccaccat caccaatgtc tcgggccggg gcgtgggcat ggatgtggtg
      781 cgccagaacg tgaaggatct gggcgggcgc atcaccatcg aatcggaaga ggacaagggc
      841 accaccttca tcctcaccct gccgctgacg ctggccattt ccgatggcat gatcgtgaaa
      901 gtgggcgacc agacgctggt ggtgccgctg acccatgtgg tggaaagcct gcgccccgat
      961 gccagggatg tgcaggggct gggcagccag cgccagatgc tgaacgtgcg cggccggttc
     1021 atcccggtgg tgtcggtggc cggctctgtg ggcgcgcgcg gcgccgtggc ctgcccgcag
     1081 gatggcgtgc tgatcgtggt ggataccgaa agcgcaggcc aagccgcgct gctggtcgac
     1141 aatatctggg atcagcggca attcgtcatc aagagcctcg atacgcatta ccgcagtgtc
     1201 gatggcgtgg ccggcgccac catcctgggc gacgggcgcg tggcgctgat cctcaatgtc
     1261 gatacgctgg tggccagcgc cgtggccgcg ggccttgggc tggaggcggc atgaacgcgg
     1321 cccctcccct ggcagcgccg cccttggccg gcgcctttgc caaggacatg cccggcatca
     1381 gcccggatgt ctatggcgag gccgattttc gcgcggtcgc gcagatcgtg cgggaagagg
     1441 cgggcatcat gctgccgccg ggcaaggcga tgctggtcta ttcgcggctg gccccgctgg
     1501 tgcgcgccag cggcaagggc accttcaagg cctttatcga gatgatccgc gagcggcccg
     1561 aagagcgccg caaggcagtg tgcgcgctga cgacgaacca caccttcttc taccgcgagc
     1621 agcaccattt cgagcatttc gcccacaccg tgcggccctg gctgctggac gagctgggcc
     1681 atggcgaagc ggtgcggatg tggtcggcag gctgttccag cggcgaggaa gtgttctcgc
     1741 tgacgatgag cctgctgggc ccggaccggc ccgccggcaa ggccctggcc agccgcgacg
     1801 tgcggatcct ggccagcgac attgccgacc acgcgctcgc caaggccagc gctgccacct
     1861 atgccgaaca ggacatcgcc gccctgcccg cccccttgcg cgaggcctgg acgaccagca
     1921 ccgccggcca ggtgacgatc ggcccggagg cgcgcggcct cgtccagttc aagcgactga
     1981 acctgcatgg ggaatggccg atgcgccggc ccttccaggt gatcttctgc cgcaatgtga
     2041 tgatctattt cgaccagccg acgaaagacc ggctggtgga acgcttcgcc catcaactcg
     2101 tgcccggcgg gttcctctac atcggccatt ccgaacgtgt gtcgggcgcg gccgagagca
     2161 tgctggatac cgtgggcccc accatctatc gcaggaggat gcgttgacga tccgcgttct
     2221 catcgttgac gattcggcca ccatgcgggc cgttctggcc gcccgccttt cgggttttcc
     2281 cgatatccag gtggtgggcc tttcgtgcaa tgccgccgaa gcgcgcgcgg ccatcaagga
     2341 actggccccc gatgtcgtca ccctcgacat cgagatgccg ggcatgaatg gcctcgattt
     2401 tctccacaag atcatgaccc tgcggccaac gccggtgatc atcgtgtcgg gcctcacgca
     2461 ggcgggcagc gatgtcaccg cccgtgcgct ggcgctgggc gcgctcgatt gctattgcaa
     2521 gacagactat gccggcagcg ccatgcagga tggcggcaag ctggccgaga tggtgcgcac
     2581 cgccgcccag gtgaacgtgc atcgcgcccg cccggtgatg cccgcccatg atgcccaggc
     2641 ctctgccctt gcccgcaccg gcaccagcgg cgataccaag gtgatcgcca tcggcgcctc
     2701 caccggcggg gtggaagcgc tgcaggtgct gctctccggc tttccggccg attgcccacc
     2761 cacgctgatc gtgcagcatg tggatgcgcg ctttgcgccc tccatcgcgc gcacgctcga
     2821 tgcttcctcg ccggccaccg tgtgcctggc cgagcccgac atgccgatca cgcgcggcca
     2881 catctatctg gccccgggcg atgaccggca catgctgctg gccggcacca gccgcctgca
     2941 tgtgaagctg cgcaatggcg agccgatcac cggccaccgg cccagcgtgg atgcgctgtt
     3001 tgcctctgtg gcgcagcggc tgggcgcgca ggcggtgggc atcctgctga ccggcatggg
     3061 cgccgatggc gcgcgcggcc ttttggccat gcaccaggcc ggcgccttca ccatcgccca
     3121 ggacgaggcc agctgcaccg tctttggcat gccgcgcgtg gccatttcga tgggagcggc
     3181 cagcctggtc gccccgctcg ggcgcattgc ccaacatgca ttttccggaa aggtggccgc
     3241 gtgatgccct tcgaccagaa acagccgggc ccgccgatca cccgcatcaa catcctgcaa
     3301 ggccaggcgc gggtgtcggc cgggccgcgc gtggaatttt caaccgtgct gggcagttgc
     3361 gtggccacct gcctgttcga tcccgaagtg gaagtgggcg gcatgaacca cttcctgctg
     3421 gcagaacccc ccgcgggcgc tgccaccggc gatttcgacg agcattacgg cgtctatctg
     3481 atggaactgc tgatcaacga gatgctggcc catggcgcca ccaagcaccg cctgcgcgcg
     3541 catctctatg gcggggccaa cctcaattcg ggctttgccc gcatcggcac ccgcaatgcc
     3601 gaatttgccc atcatttcct ggccaaggag cgcatcaccg tggtgcgcga ggatctgggt
     3661 ggcatccatg cccgccgtgt cgatttccgc ccggcgcgcg ggcaagtgcg ctgccgcacg
     3721 gtggaaaaca cccttgcccc cgaaccccgc cccatgccgc gcccgcaaaa ggcccgcggc
     3781 gatgtcgaac tgttctgaag aaagcgagcc gagcccatga acaagaatac ccgtatcctg
     3841 accgtggacg acagcgccag catgcgcgcc ctgctgaacc acgccctcac cagccagggc
     3901 ttcgacgtgg cccaagccga agacggcgaa gtggcgctgg aatggctggc gctgaacgag
     3961 gtggatgttg tcatcaccga tatcaacatg ccccggctcg atggctttgg cctgatcgag
     4021 cgactgcgcc agggcaaccg ccaccgcgac cggccgatcc tggtgctgac cacggaaagc
     4081 tcggaagaga agaagacccg cgcccgccag gccggcgcca ccggctggat cgtcaagccc
     4141 ttcgatccgg aaaagctcgt cgcggcggtg cgccgcgttg cgcattgatg ccccgccaag
     4201 gagacagccc atgcatcgtg aactgatcac attcgaggtc ggcggccagg tctttggcct
     4261 cgacatcatg gccatccgcg agatccgcgc ctggaccccc accacccgcc tgccgcatgt
     4321 gccctattat gtggccggcg tggtgaacct gcgcggcacg gtgctgccgg tgattgacct
     4381 cgccgcccgc ctcggctggg aagccaccga gccgacgccc cgccatgcca tcatcgtcac
     4441 ccagctgggc gccaaggcct gcgggctgat cgtcgattcg gtgagcgata tcgtgacgat
     4501 ctcgcaagac cagctgcagc ccccgccggt cagcgcgaat gacagcatca cccatttcct
     4561 cgaagggctg gccgccatcg acgaacgcat ggtgatggtg ctgaacctgg ccgcccttgg
     4621 cgatggcgat caatttggcg aggccgcatg atgcaggacg cccatatcgc cgcgcacctc
     4681 accaccgtgt ccgatatcct gcgggaactg gccggcgaga tcgaggaact gggcaccgtg
     4741 ctgtgccttg agccatcgct tgtgcagcgc catatgggca ccttgcaggc gatcgatctg
     4801 atcgcccaga agcagcgctg gctggccacg ctgctgaagg ccgattgccc gctctcggcg
     4861 gtcagcacca tcggcgtgga agcgctgcgg ctgcgcttct cgccctccag cgggcccaac
     4921 tgagcggcca tttacgcatt atgcccgggg tggcaggcac ggctcgctaa ccatcgcgtg
     4981 gcataagaaa agggcccgaa ccggccggcc gccttctccc ggcggccagg ccctttccgg
     5041 cccaagcccc gccttgcccc agccgcccgt ccctcttttc cggctggggc acattttttc
     5101 ccgccctgct cagtgcagcg cgctgggggc ccggcgcacc cccggcgaca ggcgctcctc
     5161 gtgatagagc ccgaccacgc gcccgtcctc ggcattcagc atcgagatcg ccgcggccag
     5221 cgccgaagtc agcgccatcg ccaccgtatc gccatccgag ctgacttcct cggccaggcc
     5281 cggcagcatc acgctggcca tgcagctatc ctcgcccgag cgcgagacca ggcaggtggc
     5341 ctcgggcggc atcagatcga aagcggcgct ttcccaggcc cccaccatca gcaaccgctc
     5401 gaactcgtcc agcccgggca ccggccaggc atcggcgggg ctggcatctt ccggcgcaat
     5461 ggcaaacagc gatcgggcca tgcgcaacat gtcggcctgt tcgccggcat cggccagcgc
     5521 gcaatcgcgg cacaattcgg ccagccgata ccgccattct cccggctgca tcgtcagcac
     5581 cgtgggcttg tccatctttt ttatccttag cgaccttatg ccccccatgt ttcgtcgaca
     5641 gcgggggccg gtcaatcaaa cgggcccgcg cggggcccgc caattgtcct tttgcgacac
     5701 atcttacgca gatttgcggg gatggccagc ccggcttcgt tccatatcgg aacgggccgc
     5761 ccctcgaccc ttaccggccc agccgctaca cccccatccc cccgccatcc gcgccgcgat
     5821 cacagatcga gcttgagcga ggaatagaag atcgtctgcc ggcgattgag cccctggcca
     5881 aagctggcca gccaggaaaa cggcccggcg atatgcacct gcgagccgat gttcagccca
     5941 tgcaccggcc ggtcgtcctc gctggcgcgg gtctgggcgt aatattccag gcccatctgc
     6001 cagcccgggc gcagctggcg gctcagcgcc gcgcccatgc tggcgtaatt gcgctggccg
     6061 gggccggggt tcagcgtgta gccgccaccg ccaaacaggc tccacttgcc ccaatccttc
     6121 tgcgcccaca gcggcagcag cacctgcgcc cgggtggaac cgcgcccggt gggcaggaaa
     6181 acccgcggga aaaaggcgac atcgggcagc agcccgcctt cctgctggtg aaggaaccgg
     6241 tatttcgccg ccagttccac atcgcctgca cccagcgggc tgccagtttc cacatgcagc
     6301 ggcagggtgg cggtcaactg caggtccttc gccccgccat agttgaggtc cacccccatg
     6361 tcggcgctgg tgatgccatt ctcatggctg cccgagacga aattgtagat ctcccatttg
     6421 tggagatcgg tcggctcggg gtcatccgtc acataagggg ggccagccag cgccggcacc
     6481 gcccagataa gcgccggcac acacacgccc gcccaagcgg ctttccccgc ccatttccct
     6541 tgcattcctg tctatctccc cgtggcctgt cagccggccc ggcgcccatc ccggagcgcc
     6601 tgctgcccct agcgcggcgc gcagctatca caacgtccaa gtaagctgcg tgacaatggg
     6661 ccaaatgggg ggaagccaaa tcagctcgat ccgcgcagca ccagttcggg ggtcatcacc
     6721 acgctggcgg tttcctcgcc ggcgatgcgg cgcagcagca tgctcaccag atgcccggcg
     6781 ccggcggcaa tatcctgccg cacgctggac agcggcggca ccgtctgctc gcccagcggc
     6841 aggccatcga agccgatcac ccgaaccgcg cccggcaccg cccgcccctg ttcggccagc
     6901 gcgctcaacg cgctcatggc gatcacatcc gatgcggcaa cgatcccgtc gggcatttcg
     6961 gacgcctggg ccaggaaggc gccaatgccc tcgcgcgcgg tttgcgccac cagcgccgtg
     7021 ggcagcacct gcagcccccc ttccagcccg gcgcgcgcca gcgcatcgcg cacgcccagc
     7081 aggcgctgct cgatctccag cgcgcgggca tcgcccagga aggcaatccg ccggcaaccc
     7141 cgggcgatca ggtggccggc cgccagatcg ccgcccagcc ggttatccac ccccaccgag
     7201 caatgcacct gcccgggcct gtagccgccc cacaccacca gcgggcgata gcgcgccgcc
     7261 acccggtcga tcgtgccggc ctgatccgac tggccgatga cgatcacccc atccacccgg
     7321 cccgaatcgg tcatccgcgc cagccagcca tcatcggccg gcaccacccg gctgagcagc
     7381 aggtcatagc cccgctcggt cagcgcatcg gccaaaaggc ccagcatggt gatgaagaag
     7441 ggatcagaca tgtgctgccc ggcttcatgg cccagcggga tcagcacgcc gatcgtctgc
     7501 gtgcgctgga tgcgcaggtt gcgggccagc acattggggc ggaaatcatg ctcgcgcgcc
     7561 agggcctgga tgcgctcgcg ggttttcagg ctgatcagct ccgatccggc cagcgcgcgc
     7621 gacacggtgc cggtggaaac cccggccaga tcggccagtt ccttgatgtt gcgaatcttt
     7681 cccatgcccc cgtgtgcccc cgtggcgcct ctttgcccgc cctgtactat gccatttgtt
     7741 ctgccgcttg gccgccattg caagcggatt tcctttgcaa atggtgtgat tggcacctgc
     7801 cccccatcgc gcgcggcctg tctctggctg gcacaggcgg atgctttgcc accttgcaaa
     7861 acggttgctg gcgggcgtcg aagaagttaa caattcctta cacttgccaa cgcccaatgc
     7921 gttcgcacca aaagggatgc gccccatgac tgacacgacc caagtgacga gccagccagc
     7981 cagcacgatc acggccgatc ctgcccctgt gggcgagcgc gaactgggcg cctttgccaa
     8041 gctgaacgcg ggcaatgccg gcgatttcat cgagcggctc gaccgcatgc agcagtatga
     8101 tgggttccgc ctttacaagg aggccagctt tgccgccatg cggctggcgc cgggcatggt
     8161 ggtggccgat atcggctgcg gcgcgggcga tgacgtgctc aagctgtcgc atctggtggg
     8221 gccggaaggc atggcgatcg gcgtcgatat cagcgccgac atggtggaag aggcgcgcaa
     8281 gcgtttcgcc ggtgcccccg cgctcgattt caccgtggcc caggctgatt cgctgccctt
     8341 tgccgatggc agcctggatg ccatccgcgc cgatcgcgtg ctgatccacg tgcccgatcc
     8401 ggctgctgcc atcgccgaga tgctgcgcgt gctgcgcccc ggcggccggt tggtgctgtg
     8461 cgatcccgac atggtgggct tctgggtgtc gagcgcggat ccgatgacct cgcagatcgt
     8521 ctcgggcgcc atcgccaaga gctgcgtcaa ccccttcctc gcgcgtgaca tgggcgtgat
     8581 gctgcgcgat ctggccctgc ccgaagtggc ccaccatgcg atgaccatgg tgaccgacga
     8641 tttcgccgtg gtggaccgcg tggtgcgctt cgaactggtg gcccagaaga tcgccgcggc
     8701 cggcctgctg cccgccggcc agatcgaggg ctggctggcc gaacagctgc agcgccgcga
     8761 cgagggccgc tttgccgccg gcctcagcgt gatgatggcc tgcgccacca agggctgagg
     8821 cttttcccgc cggaacaggc gcggttgacg aaattctaac catccccgcc ctaatccggg
     8881 gatcatggta accgcgcctg tcaccatcct tgccgatacc gcccgggtgc gcccggccga
     8941 tgtgctggcc tatgcgctgg ccctgccgcg gctggcgccg ctgcgcgtgc ccgatctgcc
     9001 ctacacggcg ctggcccggc tgtcggcgct ggcgcccggt gcgctgccgc cgctccacag
     9061 cttcatcgct tccgatggcg cgcggctggc ctatcgcagc tatggcccgc gcgccgggct
     9121 gcagctgctg ctgatccacg gctcggcctg ctttgccgac cagctgcacc acctggccag
     9181 ccatatcgcc agccagggcc tggccgctgt ccacaccatc gacatgcgcg gccatggcca
     9241 gaacccgcgc gaccagcacg cgccggccaa ccgctttgcc gccgatgtcg ccgaatttgc
     9301 cctggccctc aaacgccaga caccgctcaa ccgcatcgtg atcggcggcc attcggccgg
     9361 cggcggcctg gtcacccagg tggcccgcct gcccatggcc cgccatatcg ccggctggct
     9421 gctgctggcg ccctatctgg gcgtggccac cggcaccgtg cgccccttct tcggcggctg
     9481 gctcaccggg atcgaccgcg caggcctggc ccgcgccctg ctggcaagcc ttgccggccg
     9541 ccccaccggc cagcgcccgc tcgcccgctt caaccgcgag gcggtgctgc atgatccgcg
     9601 cttcgtgcgc acatggggct tcgagacggt gttcggcttt ggccccggct ctgtggcagg
     9661 caccggccag ccctatatac cggccagcgc cccggtgctg ctgctggcgg gcgatgccga
     9721 caactgcttc ctgcccgccg cctatccggc cgcgctggcc cgcatcgccc cccatggcga
     9781 agcggtgatc ctgcccggcc ttggccattg ggacatcctt gcccagcccg aagcgctggc
     9841 cgccaccgcc ggctggctgc agcgccattg gagcaagggc cacgcattca aaaaagaaga
     9901 ggtcgccaat gccaattgat atctgcaagc ccttgccacg atggggcgcg ctggccgcgc
     9961 tgctgttgcc cctgcccgcc cttgccgccg acgacagcgg ctggcaccgg ggagacttca
    10021 acctgcggct gggggccgcc ggcgtgatgt tcggcgaatc cggccgggtg agcgtggccg
    10081 gcagccccgt tgccggcagc aacatcaccc tgaccgacaa caccaccctg gccggcgaga
    10141 tcgaatattt ccccaccgcc catctctcgg tagcggtgaa tttcggcatt ccgccgcaaa
    10201 ccaccgtggg cggcgccggc acgctggcgc cgctgggcca gctgggcaag gtgtcgtatg
    10261 gcattggcgc ggcgatgctg cgctatcacc tgtttgccca cagccgcatc tcgccctttg
    10321 ccggcgtggg cgtgggccat ttcttcccca ccgccaccca tgatggcgcg gtgaccggcc
    10381 tcagggtggg cacggccacc aatgtggcgc tgcagggcgg gctggatgtg catgtgaacc
    10441 gccatctcgg ggtctatgcc gatgtttcct atgccccgct gaagaccacc gccaccggct
    10501 tcatcttggg ctttcccgcg ctggccgacg tcacgctcaa ccccaccatc gtgcagggcg
    10561 gcatcgccta ccgcttctga gcgccaccat gcggaacgcg ctggtttagc cccctcgcgt
    10621 tccgcccttc ctgcgccctg atcatcccgg ccggcggccc tgtggccacc ggcccttggc
    10681 ctgccgcccc tggcgcggcc ccgcgcgcga tcgcgccgcc cccacacccg ccgccgcccc
    10741 cttgcgccgc ccttgcgccc cttgcgccgc cccccttgcg gccggcctgc cacggcgggc
    10801 gaaaaaatct gtgcgaaaca gaaaaatcct gcgggcgccg cgccgccgga tcggccgcgc
    10861 cggccgctgc tgcccgacaa ttgccgtagt cctacgaggg ttgcgattaa aagaaaaaaa
    10921 ccgaaatcat gcaagaaaga cacgcaacac aatgattagg ttaaaatgct tattcacaag
    10981 cattttaagt atttatccgc gttgcaaggt ttttcaggat cggtatcgat ccataaaacg
    11041 cccgaaatgg cgcaatatta cggcctgaaa ccgcgtcgtt ttccataagg atttacacat
    11101 gaccatcgtc ctgcgtatcg cctcgcatga aaccggacgc cacgttacca aaaagctgca
    11161 caagggaatg aacgacatcg ttctgccggc cggcgcgcat gtcgaagtgg cagatggcga
    11221 cgggcaggcg gtcttgcggc cggtgaaggc ggaattcgtc cagggcaagc tgatcctcga
    11281 ttacgcgcag gaggatggca ccgtgcagct gcgcgttgct ggcgccgcgc tcgaggggct
    11341 ggacagcgcc gggccgctgg agctggccgc gctgcacacc gctttcgaca gcaccgtggt
    11401 ggccggcacg gattgggccg gtgccggctg ggccgatgcc gactggagcg gcgccgatgg
    11461 ggccggcagc gcgggcgcgg gcggcaatga ggacacagac gatagcggcc accaccacca
    11521 tggcctgtcc accacgctgc tgttgctgct ggggctgggc gcggcaggcg gtgtggcggg
    11581 cgccgtggcg ggcggcggcg gccattcggg cagtggcggc agcagcgcga ccgcgccggc
    11641 aacgccagtg ctggcgctgg gcacgggcat cggcagcaat ggcgccaatg ccaccgaggc
    11701 cacccaggcc agcggcgtgg tcaccgtcaa cgccgcctcg ggcagcagcg tcgatgtcac
    11761 cttcaccaat ggcagccaca ccgtcaccaa gacggtgagc ggcaccggca gcgcggtggc
    11821 cgtggtgctg acccaggccg atctggcgac gctgggcgat ggcaccatct cggtgagcgc
    11881 agtggccaag gccaatggcc tgtcgagctc ggcggccagc gacaacttcc tgctggacac
    11941 cacagcgcct gccgccccgg tgctggccct tgccagcgac accggcagct cggccaccga
    12001 tcatatcacc agcaatggct cggtgaacct caccgccgag agcggcagca gcgtggcgat
    12061 caccttcacc aatggcagcc acacgctgac caagaccgtg accggcaccg gcagcgccca
    12121 gaccattacc ctgagcagcg ccgatgccgc cacgctgggc gatggcgcga tcagcgtggc
    12181 ggccgtggcc accgatgcgg cgggcaatgc cagcaccgcc ggcacgctgg cctacacgct
    12241 ggagaccgcc actaccgttg ccgtcaccag ccagccgatt ctggtgggcg gcaacccggt
    12301 gctgagcggc accgccgcgg ccggcgacac gattgtgctg acgatcgccg gcgccaccta
    12361 ccagaccacc gcgaccggcg gcatctggtc ggtcgacacc ggctctgccg tgccggtcag
    12421 cggcacgctg gcgcttaacg ccaatggcac caatgccgtc agcgtgcagg ccaccgatgc
    12481 cgccggcaac accgccagcg ccagccagag cctgcagctc gtctcggcca gttcgggcgt
    12541 ggtcaacacc gatgccacca ccgttgccgt gcaggtcaat gcgatcaacg ccaatgtgcc
    12601 gatcaccatc aacgccccca acgccacctc gctgacgatc accggcaaca ccaatggcag
    12661 cgaggtcgat ctcgatatcc ccgacaagac cccgggcagc gtggacacct acccgctgac
    12721 gctggtgacc aatgtgacgg cctccagcaa cccggcccag cccaacctgc tggtgatcga
    12781 ttttgcctcg gcgcaggatc gggtggtgct gtcctcggcc agcagcatca agggcttcaa
    12841 ccagatcgag gtgcgcaatg gcacgctcga tctgtcgggc gtcaacatca ccggcattgt
    12901 cgatatccag atcaattcgg gcgtggtgct cacccttgcg cagttcgaga gcctgtcctc
    12961 gctggtcagc ctgtcgggcc tggggcagct gaccatccat ctcgacacgc aaagccagat
    13021 caacgacctg atcagcttcc tccagagccc gggcaacctg ttcctgatgg gcaccacggt
    13081 gaccgtggaa tatggcagct cgaacacggt gatcagcaac agcaccgcgg ccagcctgat
    13141 cagcgcgatg gattatccct cgatcccggc cattgccagc gcgatcagcg cgctgcaatc
    13201 ggccgtcagc ggcaacacca cctcgatcgc cctgctgatc agcgatgcct ccacccataa
    13261 tggcacgccc accaccgcct ccatcgccgg cctgcaggca gcgctggccg ccctgcaaag
    13321 cgaagtggcc cagcagcagc tggatctgga agcgatccag gcgcacaacc tcgattcgct
    13381 gcagacgctg gtgaccaacc tgtcgaacac ggtgacagcg ctcaccgcgc aggtgaacgc
    13441 ccaggccagc gagatcgccg ccaacaccag cgatatcgcc acgcaagcca accagatcgc
    13501 cagcctgctc accagcgtcg gcgcgctgca gaccaccgtc agcgcgctgc agaccggcaa
    13561 cagcagcaat gccgccgcga tcagcgccct gcaaaccgcg caggccagcc ttgccgccac
    13621 cgtttcggcc ctgcagacca cgctgcagaa cgatatcgcc agcctggaag gcaccaatta
    13681 cgccaacgcc acctacaagg acatcgcctc gctgagcgcg cagatcgttg cgctcaacag
    13741 tgccgttgcc gccctgcagg ggctggtggg gcaaagcagc gtggccgcgc agatcagcgc
    13801 ggcaatcggc catcccacca acagcagcgg cgcggctacg ggcctgtggg tcagcatcga
    13861 aagcgcgatc agcgccgctg ccgccagcct gcaaagcgag atcaacagca ccaacgccaa
    13921 tctggcgcag gtgatcgatg attccagcac ccatggcggc acgcccacgc tcaattccat
    13981 cgccggcctg ctggcggcgg tgaatgtgct gaacggatcg ggcgcgggct cggtctcggc
    14041 gcagatcacc gcggccatcg gccaccccac caacagcagc ggcgcggcca cgggcctttg
    14101 ggcgggcatc gaaagcgcga tcaacgccga agcggccacg ctgaacgccg agatcgccgc
    14161 cctgcagggc gccaatttca acagcgccac ctatcaggat atcgcctcga tcagcgcggc
    14221 catcaccggg ctgcaggcgc tggtgggcaa cagttccgtc tccagccaga ttgccgcggc
    14281 gatcaatggc gtaacgagca gcttcgacac gctgatctcg gccctgcatg gcgccggcgt
    14341 ggtgctgacc gtggccgaaa ccgcagcgct gctggtctcg gacccggccc agctgctgac
    14401 caattaccgg ctggtggaca gctatgccaa cctcttggcg gccacgcaga acgcggccga
    14461 tgcccatgtt tttgcctatg ccaagacgat cggcttcact gccacctcgg cgctgagcgt
    14521 ggcccaggtg agcgggctgg aaagcgcgat tgccgccatt gccaatgccg cgcccgtcag
    14581 cgccgcgctc agcagctaca gcctggccga tacggtggcc gatctggccg cgggcagccc
    14641 ggcgatcctg tccggcgcca ccaacatcac cgccacgctg ggcagcgcca gcctggccca
    14701 ggtggccagc gtgctggcgg ccaccaatgc cggcaccacc gtgctcgatg cggtggcggg
    14761 cacgccggcg cagatcctgg ccctgccgat cagcgcgcac gacacgatca ccgccatcac
    14821 cgtgaccggc cttgccaccg cccagcaggc cgcagacctt gaacagctgg ccagcgccgg
    14881 ccgggccggc agcgtgcagc tgggcgccgt ggccgatacc gccgccaaca tcgaggcggt
    14941 gatcgccagc cagaacggcg ccaccttcct cgccagcgcc agccgcatcg atgtgtcggg
    15001 cacgctgagc gtggccgagg ccgagcagct gcaggcagtg aacgccaatg tgcacatcac
    15061 cgcccttgcc gacagcgccg cctcgatcac cagcgcgctg gccgccagcc agctcagcct
    15121 cacctatgtg acggcgctga gcaccaccga caccgccacc atcgccgagg ccagcgccct
    15181 gcaggccgcc ctgcccggcc atgtctcgat cctgggcatc accgacacct cggccgatat
    15241 cggcgcggca gtggtggccg gcacgctcag cctcacaggc gttggcgccg ttaccatcgc
    15301 cgatggatcc accgccgatt atgccagcgc cgcgcaaggg gcggcgctgg gccgcagctc
    15361 gctggcgctg gcctacgatg tggccgacac atcggcccat tatgccgccg ccatcgccac
    15421 ctatggccag ggcttcctcg ccaaggcgga caatatcgtg ctgacgggcg ccgcctcgat
    15481 cgcagtggcc cgctcgctgc tgggcgcgca gaacagcggc accgtcacca tcgacacgat
    15541 caacgccacc gccgcgcaag tcacctcgct caatttcgac gcgcatgaca tcgtcagcca
    15601 cctcaacgtc gccggcctca cctatggcga ccagacggtg gacctgacca atgtgccctt
    15661 ctccaccaac ctcacctata atggcgcggc gctggccagc agctacagcc tgctgctcta
    15721 tctgccccag cccgatgggg tgaatgtcga cgagaacggg gtcgatcagg ccggcgaagt
    15781 ggtgatccac gccggatcgg gcaacaacac cttcgtcttc accgacgatc tggccgatgg
    15841 cacctatctg cccaccgcct atgccagcgg ctcctcgatc gattatgcca cctatggcgc
    15901 cggccagcac ggcaccgaaa cgctggtggt gatcggctcg gtcgatatct cgaacatcac
    15961 gctgaacggc gatatcaaca gctacgatct ggaagcgctg gccggctcgc cctcgcaggt
    16021 cacgctgtcg gcccgccagc tcaacatgct gcacaatttc accggcgcca gcggcggcac
    16081 ggtgatcaac atcaccaatg cccccgatcc ggccacaggc accgtggtgg tcaccagcct
    16141 cagcaacaac gcgctgacgc agaacatcaa tttcaccaat gtgacgggcg tcaacattgg
    16201 catcaacgac aatctgcaga tcgatttcct gcgcctgctg ggcctttcgc atgtgacgac
    16261 ggcctcgggc tcgggcagca gccaggcggg gctggtcacg ccggtcttca acaccgccgg
    16321 cggccagacc tacctcacca ccgatggcac gcagaacggc acgccgatct cgcccagctt
    16381 ccttaccaac gcctcctaca acatcgacat gagcgcggcc gcgggcctca gcctcggcaa
    16441 tgacggcgcc ggccactact acctgcagga cagccagggc aacgagctga acctgccgca
    16501 aaccctcaac ggcagcacgc tcgccctctc gcccgacgag atctcgggcc cgggcgcgcc
    16561 gctggtgatg ggcgccacca atggcggcgt gctgcagctg accggccttg tcaattccgc
    16621 cggccatgcg gtggacgtga cccaggtgag cgcgccgatc accctgtcca tcggccagag
    16681 caccgatttc accagcgcca gcaaccccgc ccagatcacc gccgatctgg cccatatcgc
    16741 cgccaccgcc ggcagccgca tcacggtcaa caatgtgggc ggcggcacgc cgccggtcac
    16801 gctcacgctc agcgccgatc aggccagcgg cctggccatt gccggcaccg gcgaagtgaa
    16861 ggtgaccggc actgccaccg gcaacgagga ttattccgcc ctttctgcca ccaccatcga
    16921 tctgtcgggc gttgtcccca atggccacag catcaccctg ccggccagcg tgccggccgg
    16981 ccacacgctg atcctcaacc aggccgaagt gaacggcgcc accatcggtg gcagcggcgc
    17041 gctgaacatc ctgctgaaca gcgctgccgc gatggatctc agccatatcg acaccagcca
    17101 cctggcggtc tcgctggtgg tgcccaccgg caccacggcc accgtgcctt ccggctccaa
    17161 cctcgccaat ttccccatta tcgtgcaaag cggcgccacg ctgaacattg ccggcgatgt
    17221 ggcttcgggc aaggcgatct cgggggcggg cacggtcaat gtcaccggca ccggcacggt
    17281 tgcgggcggc gacgatttca gccagatcac cgcccacacc gtcgatctgt cgggctatag
    17341 cggcgctatc gcccatctgc cggcgctgtc caccagcggc gtcaccgctc tggtgctgaa
    17401 caccgccgag gccgatttcg ccgcctctgc cccggccaat gccctcaccg tgcccagcgg
    17461 caccggcatc accgtagtgg ccaatggccc ggccagcgcg ccgggcacca cgctcgacct
    17521 gtcgggcatc cacaccaccg gcaccggcac gctggccgtg catgtgaccg gcgacaccgt
    17581 gctggccagc gccaatctca acggcgcgcc ggtctcggtg gacagcaatg tcacgctcac
    17641 catgccggtc ggcgcggccg gcgggctgtc tatcagcggc gcgggcacgg ttgaccttac
    17701 gggcgggggc gcggtttcgg ccgatctttc gcacctcacc accacccagg tgaactggga
    17761 cgtgagcgag aacaccacct tcaccggcac gctgggcacc gctgtcgtca ccatcgatca
    17821 tgacgtgacg ctgagcggca gcgccgcccg ctttgccggc gcccaggtgc tggccggcgc
    17881 ggtggataca gggctgggcc agagccgggg cgtgctctcc atcaccggcg ccttcaccaa
    17941 ccaggatttc agcggcgttg ccgccagcct cgatctcagc cacgccactt caggcacgat
    18001 caacaacctc accctgccca catgggtggc aggccaggcg ctgatcctct cctacctgca
    18061 gggcgacggg ctggtgctcg atgtcggcgc gggcgatgcg cgcatcgtca acatcgtcgc
    18121 cgatgccgcc tccgacctgt ccggcgtcac ctccaccagc ggccatgtca ccgccatcat
    18181 cgatggcacc gccaccttca ccggcacgtt cagcagcgcg gccaacgcct cgatccattt
    18241 cgtcaccggc acgggcggcg ccacctacac gctcagcctc gatggccacc tgctggatgg
    18301 ccgcacggtg gatggcagcg gcacggtcaa tgtcaccagc ctgtcggcca gcaccgattt
    18361 cagccttgtg gcgcccacca tcctcgattt cagccatgat ggcgcgctgg cggtggtcag
    18421 cggccagctg agcgagaatg gcgcgatcgc caccctgccc agcctgaccg ccggccacac
    18481 gctggtcgtc accgccgccg aggcgaacac gctggcggtg agcggcagcg gcggcacggt
    18541 ggatgtctcg ggcgatgtgc ccgccagcac cgacctcacc ggcattgccg ccggcatcgc
    18601 catcagcttt gccgacagca ccggcgcgca ggcgatcatc gccgatggcc agcagctgac
    18661 catggccagc gaccagctga ccggccagac gatcaagtcc gacaccgccc acagcgcctc
    18721 gggcacgctg atcgtgcagg ggcgcggggg cagcggcacg gccctcacct cggcggccga
    18781 tctctccgcc gtcagcgcca atatcgatct caccggcctt gccaaccttg ttgtcgcagg
    18841 cggcctgctg gaggatgcca ccaccgcggc ctatgccctc accctgcccg cgctggccgc
    18901 cagccagacg ctgctggtcg atgccggcca gctgggcggc gatgcgctgg cgctgggcgg
    18961 caccggcacg atcgacgtgg aaggcgatat caccaccgtc tcggtcaacc tgtcgggcct
    19021 gggcgatggc gtccatctct cgctgcagga cagcggcggc aatgccatcg acatcggcag
    19081 caacctcagc ctgacggtga aggcggtgca ggcctcgggc caggcgatca ccggcgccgg
    19141 cagcctggtg gtggtggacg acaccaccca tgccgcagcg ctggtggccg atctgtccca
    19201 ggtgaccagc acgatcgatc tgcgccaatt tgccgggctg catgtggatg gcggcgccgc
    19261 gctcgatcag ctggtcgatg ccaccggcac gctggcgctg gcgctgccgg tgctggccag
    19321 cggccaggtg ctgctgcttt ccaccgccga gctggctggc ggcctgccgc tgcaggtgct
    19381 gggcaccggc gtggtggaga tcatcggcac gctggataac cagtcgatca acctcaccgg
    19441 catcgccgcc ggcaataccg tcgatctcga cagcggcgcg ctgacgatga accacggcgc
    19501 gcagttgacc gccaccgccg gccagctcag cggcaagggc atcaccgccc tcgacagcgc
    19561 cagcaccgtg gttgtggccg atgccgccac cacgccggtg ttgacggccg ctgccatcag
    19621 cgccgcgctg gcagccaacc ccaatgcggc gatccttggc aatctggtcg acaccgccgc
    19681 caaccttgcc acgctggact ggacggacaa cgccttcctc ttcagcgtgc acggcacggt
    19741 gaccgccacc accgccgcca cgctggccga gacggccgtg ctctacgcca tcaacttcta
    19801 ccgcgccggc atggccagct tctcggtggc cgacacgctg gccaattttg ccgccgcccc
    19861 gcaatggctg gtggaacagg cggtggagcg cgcgtcggtc aatggcggca cgatcaccgt
    19921 caccgatccg gtctcgctgg ctgatgccag cgcgctggtg gcagacaatc tgctctttgc
    19981 cgccaacaac cagaaccagg tggacgggca gaactggaac ggcatcacct tcaccgtggc
    20041 cgacagcgcc gccgcactgg ccgcgcttgg cgccggcgct gcggccaacc tgcaggccgc
    20101 gctgcaattt ggcggcggca gcatcaccat caccggcacg gccacggccg cgcaggcctc
    20161 cagcctggcg gcggcctatg gcagccttgt ccattatgcc ctggccgata gcggcgcggc
    20221 gcttgttgcc cgccacacca ccgatggcaa ctggaccttc ctggccgatg ccagcgccac
    20281 cgaagtgacc ggcagcacca gcgcggccaa tgccatcacg ctggaaggcg ccggcgcgct
    20341 caccatcgat ctggtgaccg acagcgctgc ggccattgcc accgccatcg gcggccacgc
    20401 ccatttcctc gacagcatcg ccggcatcag cctcacctcg gcggtggcgg tcagcacgct
    20461 gggcagcgcg gcggcgcaaa gcgtcaatta tgtctccgac acggtgagca acctcttggg
    20521 catgggcatc tctgcctata cccgcctcac cgccggcgcg gtgatctcgg ccacgggcac
    20581 cgccaatgcc tctcaggcgc agacgctgat ccagaatttc tacaatgatc tcaacggcgg
    20641 cggcaattat cccaatgcca ccaccgaaaa gagccgcctg ctgctgagca ttgccgacac
    20701 cgccgccaac atcaccgcgc tgggcgtggc cgatctggcg cggctgtcgg gcgcggtgca
    20761 tgtcaccaat gcggcgagcg ccacgcaagg ggtggaactg gcctggctgg tcgccaattc
    20821 gggcgcccat gtctattatt cggtggccga caccgccgcc aacctcaccg gcgagctgac
    20881 cggcgccatg gccgcctatg tgaccggcac gatcaccgcc accaccaccg ccacggccgc
    20941 tgaggcctat gccctctatg ggctggccac ggccagcggc gtcagcgccc cggtggtctt
    21001 caccctggcc gacagcgtgg ccaacatcga ggcggcgatc accgcccatg gcgcaggctt
    21061 cctggcccag cccggcacca tcgtcgtgac ggatgccgtc tcggtggccg aggcgagcgc
    21121 cattctcgcc gctgccggcc agaatgccac cgtcaccttc accgcgctga ccgacaccgc
    21181 tgccgatgtg aacggcgcca gcgcgggcct gctggcccat gtcagcggca cgatcaccaa
    21241 ggtgttgagc gcggccagca ccgaccttac cggcgtgagc gccaatatcg acctgcgcgg
    21301 ccttgccacc cagggcgtgg cgctggatgc cggcacatcg cgcgccacca tcgtcatcaa
    21361 ccagctggtg gccagcagcg gcgctgttgc cacgcgtgag gcctatgacg tgctgctgcc
    21421 cacgctggcc gccacgcaga cgctgaccgt cgatgccgcc gagctggccg gcagcctggc
    21481 cgccgccatc gccggcagcg gccagctggt gatcgccgga gacatcgccg ccgacaccga
    21541 ccttaccggc gttgccgccg ccatcggcgt gtccttcaag gatagcggcg aaagcggcgc
    21601 ctttggcacg gtggcgctgg ccgccagcgc ccatctcacg gcctatgacg ggcagatttc
    21661 cgcccatgcg atcaccggcg ctggcacgct ggtggtgctg ggcggggcga gcggcggcca
    21721 tctgtcgggc gcgctcgatc tctccaccgt cagcgccagc atcgatctca cccaggcgct
    21781 gctctcggtc gacacggccg ccggcgcgca tcagggccag ctggtggaca atggcagcca
    21841 tgtcaccctg cccaccctca ccggccagac cgtcagcctg acgatcgccg agctgaccgg
    21901 cgagctggcg atcagcggca ccggcacgct ggacctgcag ggcaacatca ccagctcgct
    21961 cgacctcacc tacctgcccg ccaccatcgc cctctccttc ctcgatgcgg gcgacgccgg
    22021 cggcctggcc acggtcgatg tctcgggcac ggcgcagagc cagacggtgc tcaccctcac
    22081 cgccgatcag gccagcgacc agttcatcac cggcaatgcc tatgccacca tcgtgctgcc
    22141 ctatgcggcg ggcgctgcca ccaacctgtc ggccagcacc gatctggccc atgtgttctc
    22201 caccgattat gccaccaacg cggcctgggc cagcgcgctg caggcggcca atgccggcct
    22261 tgccgggcag atcgccgcca cgaccaatgg cgtggtgctg accggcggca acagcgacgg
    22321 cgccttcgag accttccagt tcaccgccca ggccaccggc acctacagct tcaccgccaa
    22381 tttccagaac aactaccacg gcgcctattc ctataccgac gcctatggca cccacatcta
    22441 ctaccccgac agcgaccgct attacctgac caccaacgtc aatggcgggg gcaaccaggt
    22501 ctatggctgg accagcgcct cgggcagcac cacctatacg gtaacgatgg tggccggcga
    22561 gacgctgtcg atctatctct acacgccagg gggccgcacc gccggcgccg atggggcgct
    22621 gaccacgccc agcacgctga ccctgtcgaa cggcggcttc tcggccgtgg gcggcaccgc
    22681 tgccgcgctc gacgtgacgg tgggccagaa caccgacctc accggctctg ccgatccgct
    22741 cgcctatgtg tcggtcgatc agttcaccct ggccaaccag gccgtgctga cgatgagcgg
    22801 gctggaagcc agcggccatc tcgtcaccgg cagcggatcg gtggtcatcg ccggcaacat
    22861 cacctcgggc tgggtcgatc tcacccaggt ggccgtgccg ctcagcttca ccaattcggt
    22921 cagcgacgag cagcagctgg gcatcaatgg cgacaactgg ctgcgcctca ccgccgccga
    22981 ggccaatggc ctgttcacca gcggcggcac ggtgaacatc ggcggcgata tcgccgatgg
    23041 cgccagcgcg ctgatcgacc tcacccacat ccagtccacc ctctccttcg aggataccgc
    23101 caccacctcg cgcaatgtgg gcgcctatac cgcgctgacc acgacgacga cgatcgcccg
    23161 caacgagctg aacacgctct cctatgccac gggctggaac tacggccaga tctatgtggg
    23221 cagcggcgac gggctggtgc tgcgcgccga tcaggccaac tggcattccg tctcggggcc
    23281 cggcaccacc tatgtggtgg gcaatctgga cgacggcgac ctctcgctgc tgggcatcac
    23341 ctcgcggctc tccttctggg acgggatcgt tgccaacccc tatggcggcg atgccagcga
    23401 ggatccgcag agcaccggca agggctcgat caccctgtcc gagcaatcga cgatggaagt
    23461 gatcggcaac cagctggatg gcaattacat cctgggcgat ggctcgaacg tggtgcgcgt
    23521 ctatggcgcg ctgggcgatg ccagccatgc gatcgacctg tcgcacatca ccaccagcat
    23581 cgaccttgcc tatgcctacg accacaatgt cacgctggtg acagggctgg acgggcaggg
    23641 caacatcacc accaacaacg gctcgatcac ggtgggcttc aacgcgctga ccggcgggca
    23701 gaaaacggtg atcgccggct ccatctcggg cagctacaac acgctgtcgg cctatctgcc
    23761 ggaaaactgg aaccccgacc agacgctgac gatctactcg cgcgatgcca acgacctctt
    23821 gctcgacacg cgcgactaca gcaacggctc gctggtgctg gacgagagcg gcaatgcggt
    23881 gggcgccaag gtgctgatcg cccatgtgga agatctgctc agcaacggcc agatcaacgg
    23941 cgatttctcc gagatcaacg cctcgctggt cacgctcaac ttcggcagcc ggcagggcaa
    24001 tgtgctcagc tatgccggca gcttcggttc ggccacggtg ctgatcgatg atggcgtgaa
    24061 tgtggtgatg gatggcgcgg tggccagcgg cgtctatttc gacacgcaat acagcggcta
    24121 tcgctggaac ggcagcggct ttggcgtggt gggcatcaac accctcaccg ccaatgccga
    24181 ccttaccaac acggtgaacg atccggccag cttcaacctc accctgccca acatctatgg
    24241 ccatgtggat atcgatctca gctatggcgc ggcaggctct gcctatctgc ccaatggcgc
    24301 gaccgcgatc acctggaatg ccgcgctggg ccagttcacc aacggcacct atgcgatcac
    24361 cctgcccagc ttcgatggcg aaaacgggct gatcgtcaac gccagccagc tgtctggcgg
    24421 caccggcttc acgctggccg gcagcggctt tgtgggggtg gaaggcaata tcgccgccaa
    24481 cctcgacctg acccagctca gcaccagctt tgccgtctat ttcggctcga acaccaccaa
    24541 catccgcaat ctggcgcatg gccagtccta tcccaccgga tgggacaact ggtccgccta
    24601 cagcggcacg gcggtcaact cgggcgtcac cctcaccctg cggcccgacc agatctacaa
    24661 caacatcgcc atctcgggcg ccggcacgct ggcgctggcg gcgggcacca gcgcctatgc
    24721 ggatgaattc ggcaatgtcg atctcatctc cgtcaccgcc aacatcgatc tcaccggcct
    24781 tggcacacag gccagcagcg gcggcatcag ccaggatggc acccgcgatg gcacggtgat
    24841 caactatcgc gagggcaaca gctggctgaa ctacaccgcc ggctacacgc tgggcaatgg
    24901 cggctatgaa ctgctgctgc caacgctggg cgccagccag agcctgaccg tgctggccga
    24961 ccagctggcc ggcgccaatg gcttcaacct gggcggcgcc agcggcgcgc tgctggatat
    25021 ccagggctcg gtaaacagcc atgtctcgct gctgaatgtg acgaccggcg tgacggtgag
    25081 cttccgcgat ggcagcgaga cgggccttgg cgccatcacc gtgaatgccg ccggccagct
    25141 ccttgcccgg gtggaccagc tcgacgggca cagcatcaat ggcgccggca ccgtctatgc
    25201 cggcatcacc ggcaccgtgc cgcagatcag caccgccgat gccagcgccc tgcaggaaat
    25261 ggccagcggc cacaccgccg atctcaccgg catcaccgcc aatctcgatc tgacggcact
    25321 gtcgggcacc aacacgctgg cgggcctggt gatcaacggc agcggtcagt tcgccgatgg
    25381 caccgatgcc accaagctgg tcaacctgcc caccctcatc gccggccaga cgctgacggt
    25441 gacggcgctg gaaatgaagg gccagctggc actggtcgag gccgcgcaga acggcggcgg
    25501 cacgatcgac atccagggcg atatttcgga tgccaccggc accgttgatc tcacccatgt
    25561 cgaggatggc atcgccgttt cctttgccga caacgccaat gccaatgtga tgctgagcgg
    25621 caccagcgcc cagctgatca tcaaggccgg gcaggccagc ggccttacca tcaccggcga
    25681 tggcagcagc gcggccgcct ataacaacgc cggcacctcg gccagccact ttaccggcgc
    25741 cgcggtggtg ctggccgaca atgacaccgg caccctttcc accctggcaa cggccaattc
    25801 ggccacggtc gatctcaccg gcatttccgc cgatctcgac ctgacccagc tgaccggcct
    25861 tggcgtggat gccaatggcg ccctgtcgga tggctatggc aagctggtgc tgggcacatc
    25921 gggcgccgcg gccaaaccgc tgcgcgccca gcagacgctg aaggtgaaca ccgcccagct
    25981 tgcctcgctc agcgccgggg gcaccgggcg cgtggtgctg aacgggcagg ccggcagcat
    26041 tgtcgatgtg cgcggcaacc tggcctcggg cgtggcgatc gatctcaccg gcatcctggg
    26101 ctccaccatc tccttcgtcg acacccagga tgtgagcgcc aatgccatca ccgtggccgg
    26161 cacgctgacc atgctggccg agcagttcga cgcggccggc ggcatgaaga tcagcggcac
    26221 cggcgccctg tggctggagg ccacccaggc caccaccacc gtcaactgga ccgacagcgc
    26281 gcaaacgccc gcctactaca tcagcaacac cagccttgcc tccacgctgg atgtgcagga
    26341 cagcaccacg ctggtcgccg ccggcagcgg gctgatgccc accgccatgc agatcaaggt
    26401 ggaagccagc cagacgctga cctcgggcta tgcctatgtc tcgggccgca ccatctacga
    26461 tgccgcccac caatacagct acaacgccca gaccggcacc tacaccgaaa ccggcggcac
    26521 gctggtcgtc accggcagtt ctgtcagcgc gctcgatctc agccatgtca ccatcggcac
    26581 gctggatctc acgcaatatg gcggcacact gttcaccggc ctgtggaccg acctttccga
    26641 ccacgtgctg ctgatgaacg acacccaggc cagcggccat gccttcaccg ccagcggcga
    26701 tggcggcgca ctgacggtga tggccaccac ccctgccacc taccacgatt tcagcggcat
    26761 cggcgccagc gtcaacagcc tgctggtggt ggggggcgcg gccgacatgg ccgatgccat
    26821 cagcggcgcc aaccacacgc tgctcaacgt cttcagccat gtggagctga attatgcgag
    26881 cgggctgctc accgtctcgg ccgatcaggt gaacggcatg accgccaatc gcttcaccgg
    26941 caccggcggg ctttatgtgc gcgatgatgc cgccacctat cacagcggcg ccgaaaccct
    27001 gtatggcagc aacaataacg acaccttcgc cggcggcggc tatggcgaca cggtggatct
    27061 gaccggcacc ggcaacaccg gcggcggcac cgacaccgtg ctcttctcca ccctggccgg
    27121 cagcagcggg cacacggtgg acttctcgat cgccggcttc accaccggcg ccggcaccgg
    27181 ccatgatgtg ctgaacttct cggccctgtc tgcccacacc gcgcatagtg gcacaggcga
    27241 tgtctcggcg gtcagcctgg cagcggtgcc ggccctgctg ggccaggaag tgatcgtggt
    27301 gacagacagc ttcggcgccg atgcctcggg cgtggcctcg gtgttcggcg cgggcagcaa
    27361 cctgctgaac aaggcgctgg ccgccgccgg caacaccgat gcgatcttcg tggtggccct
    27421 ggcagacccc aacaacagcg gcagcggcgc gccttcggtc aacttcggcg tgtggcactg
    27481 gcaggaggcc agcggcggca acggctatgt ccatgccgcc gaactgaccg agattggcgt
    27541 gctgcacagc ctgaaccaga cgcagatcgc ggcgatgacc agcgccaacg tcatcggctg
    27601 atccggcccg ggagcggggc cgccttccgg cgatgccccg cccccggccg acggggcatc
    27661 gcccctgccg caaaatgcaa tcattctcga tctttcgctg cttccccaga ggaggcccgg
    27721 cggcacacaa tccacgccac cgcatgcgat ccgcgcgcac ctggggcgca tttttccgtt
    27781 catttctgat tcaaaacgat tttttggcga atgtggggga tggggcaagt gggaaacggg
    27841 cacaggccaa ccccgttcgg ggcgctgcgg cagggagcgg cacggggagg cacgctggca
    27901 gccgccggat ggctggcgct gacaggcggc atcgcctcgg catcggcgtc aatgggagcg
    27961 caaacggagg ggcaggcgcc agcgcaggcc cccgccgccg ggcaggccct cttcggcccg
    28021 cccgcgcccg ctggcacgcc cgacggcggg gaagacgggc tcgatccggc cgccagcggc
    28081 ctgcccacgg cagaggacat gcaacctgcg gccccgcccc agccccagcc catgccgctg
    28141 ccagaagccg cgccgcccct gcacaccgcc cctgccgccg gcttcaaccc cgaactggcc
    28201 gggctgatcg aggaagcggt gcaccgccat ccttccatcg cctcgcagcg ggcggccctg
    28261 cgcgccgccg gggtggatgt gaccgccgcc tggctcaccc gcctgcccac catgtcgatc
    28321 cagttcaacc agtatggcgc catcgcgctc agccggcagg tcactgccaa tgtcgatctg
    28381 ccgctgtggt cgaacggcaa gatcccggcc acgatcgacc gggcgcgggc cagccggcag
    28441 gcgcagttct atcgcctggc cgaaaccgtg ctcgatctcg agctggaagt gaaccagtat
    28501 taccacgaga gcaaacgcct ggccgagcat gagcaggtgc tgctgcgcat tctggcggtg
    28561 atggacgaca tggtcgcctc catgcgccgc cgggtggcgc aggaagtgag cccgctggcc
    28621 gatctccagc tggccagctc gcgccggttg caggtggcgc agcagctcga tctggtgcgg
    28681 gcgcaaaagg ccagcgccca ggcccatctg gcagagctgg tgctgcgcga ggaggtgccg
    28741 ctggcgccgg gctttgtcgc cccgcccagc tggccgcaat ggcagctgcc agcgctgacc
    28801 gagcggatga tcaccgccag cccgcagcgc cgccgggtgc aggccgaagc gcaagtggcc
    28861 cgcgatgatg cgcggatcac cgccgcctcg cgcctgccgg gcctgttcgc ctcctattcc
    28921 tacgacgagg tctacaagca tcgcgtgggc ctttcggtgc gcatgcagac agcgggcggc
    28981 ttctcggaga tcttctccac ccgcgcggcg cgcctgcgcg aacgggccag cgacctgcag
    29041 gccccggccg tggagcagga catgaaagcc accgccagca acgatctggt cgaatatgat
    29101 tccgcccgcg cccgcgatgc cggcgcgcgc gaactggccg acgacagccg caagatcacc
    29161 gaaagctatg tgcgccagtt cgtcggcggg cggcgcacat ggctcgatgt gatgaatgcc
    29221 gtgcgcgagg cgatgagcgc cgatctcgac gcgatcgacg tgcgctacac cgctgcggct
    29281 gcggccatgc gcattctgct gcgcagcggc gataccaaaa ccattgtgga aggacgaccg
    29341 aagtgacgct gcccctgctg gatctggtgg cggcgctggc ccgccggcgc ggcgcgcgcc
    29401 ttgcgcccga ctggcaggcg gccgttgccg tcgatcttgc gctggacgat ggcgaggcgc
    29461 cctggcggct gagcgaactg gccggctggg aagccccggt ggtgctggcc gcgcgcccca
    29521 agccgcagga ttttcccctg ctggtctatg tggaggcgct gggctgggcg ctggccgaac
    29581 aatggcaggg gcccgatcac atccgcgcca tcaccgccag cggcctggcc gaggtggaat
    29641 gggggccggg gctgcggctg gtgcagctct ccttcccgga agcggcgggc acgggggaat
    29701 atccttcggc cttcgcggtg tttcgcgcgg ccatcctcgg ccggcggcgg atgatcgtcg
    29761 atgcaacggc ggccacggtt gtcatcaatg tgctggcgct gatcacctcg atctattcga
    29821 tgcaggtgta tgaccgggtg atcccgcgcg ccagctacga cacgctgtgg gtgctgacag
    29881 cggggatggg cctggccctg ctgatcgatt tcctcatccg caacacccgc gccttcctga
    29941 tcgacgagga agccagcgag atcgacgcgg aagtgtcgga atatttcttc gcgcggatgc
    30001 aggcggtgcg gctggatgcg cgcccccccg gcgttggcac catggccggc cagctgcgcg
    30061 ggctggaaca ggtgcgcgcg gtcatggcct ctgcctcgct gttcattctg gccgatctgc
    30121 cctttgccct gctgttcatc ctggtgatgt atgtgattgg cggcaatgtg gcgctggtgc
    30181 cgctgctcac cttcccgctg gcaattggcg cggcgctgct gttctcgcgc atgatccgcg
    30241 aggatacgca caagtcgcag gtgtcgggca accgcaagaa cggccttttg gtggaagcgc
    30301 tgggcgcggc cgagacgatc aaggccaatc tgggcggctg gcacatgctg gccggctgga
    30361 accggctggt ggaaaccgtg caccggcacg acaatcgcgt gcgccgctgg tccaccctgt
    30421 cgggcagcac gttcaatgtg ctgcagcagg tggcctatgt cggcatcatc gttgtcggcg
    30481 tgttcgagat tgccgaccgg cagttgacga tgggcgggct gatcgcctgc acgatcatca
    30541 gcgggcggat caacgggccg ctggtggcag cgctgcccaa cctcatcgtg cagtggagct
    30601 atgcgcgctc ctcgctgcag gcgctggaca atatcctgcg cctgccgagc gaccatccgc
    30661 atgaccgcca gatgctgcgc ctggccgaag tgcgcaccgg gctgcggctg gccaatgtgc
    30721 aattcgccta tccgggcgcg cgttcggggc tggagatccc cggcctgttc atcaaccggg
    30781 gcgagaaggt gggggtgatc ggctcggtcg gctcgggcaa atccaccctg ctgaaggtga
    30841 tggcggggct ttatgcgccg gcgctgggcc aggtcacgct cgacggggtg gaagtggcgc
    30901 agatcgccga ggatgacctg cgccgccggc tggtgtgcct gccgcaatcc ttcgagctgc
    30961 tgagcggatc gctgcgcgac aatctggcgc taggcatcgc caatccgaat gacgagcggc
    31021 tgctggccgc tgcccagcgc attggcctgg ccgagctggt gcgccagcat ccgatggggc
    31081 tggacctgcc gattgccgaa ggggggctgg gcctgtctgg cgggcagcgg gcgctggtgg
    31141 ggctgacccg cctgctgttg accgacccgg cgatgctgct gctcgacgag ccgaccgcca
    31201 accttgatcc cgaaaccgaa ttgcgcgcga tgcagcagct gttcggccat ttcgccgacc
    31261 gcacggtggt gatggtgacg cacaagcccc agctgctcac ctttgtcgag cgggtgctgg
    31321 tggttgtcga tggccgcatc gcgctggatg gcaagaccgc ggatgtgctg aaccagctgc
    31381 gccccaagcc ggcgccggcc agcggccgcg aagtgcccga tcatcgcccc acccaggaaa
    31441 aggactgaca gcgccatggc aaacggcagc aatttgcgcc tgatcggcaa tggcgggcgg
    31501 ggccagcgcc accggcgcgc gcccggcccc ggc
//
LOCUS       DASRYE010000047         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_54678,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000047 DASRYE010000000
VERSION     DASRYE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54678"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFF98_02795"
     CDS             complement(<1..271)
                     /locus_tag="VFF98_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU62585.1"
                     /translation="MRDFAGKSAIVTGASRGIGLAVARLLAARGADLVLVARAEAPLR
                     AAAAAICAESGVRCLAVAGDGAQTATAEAAVARALAAFGGLDILAN"
     gene            complement(283..642)
                     /locus_tag="VFF98_02800"
     CDS             complement(283..642)
                     /locus_tag="VFF98_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62586.1"
                     /translation="MACCLLAALLFAHVMATLRRWAVFWGLARPRAGEEADTALGHLR
                     GWLARPAVRVGLACLFALEIGLGGWWVYTRHGAHLYRLADSALARSRGELIVYAPVCT
                     GGRADAGQRVRVALALR"
     gene            complement(645..>2268)
                     /locus_tag="VFF98_02805"
     CDS             complement(645..>2268)
                     /locus_tag="VFF98_02805"
                     /inference="COORDINATES: protein motif:HMM:NF037273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62587.1"
                     /translation="AFAWILFIWWARGVILGPPAVLLGSALLSAAWVGARGGVPAVAW
                     GLPAGVIGLFLWLALQRRRHFAAQQARGAQLNQILARTAPILRDRPVAAADEMPDGQV
                     AEFRRMLDLALQPVDSWTGFAMNDTWQDGALRYQICTMSWNLAFGQYTQLPAFHGYLN
                     QAQHNLIAKHIDRRTWNYWFWESLWGNFQIQKNPVAIDNIMLSGFLGVSLGLFETASG
                     TAPFAAPGALTFRWDEGTAFAYSQETLLEEVVRNFRRYDLGWFPCEPRWIYSMCNLVG
                     RTALALHDARHGSDMIARIGDRFERTMAEEMLMADGRIKVCTSSPFGFTVPSLSGLFG
                     ETWGIRFLTPHAPDQAERLWQVLKQDFIRPAPDGTLDYKLLPLGWDTRKPANFSYDQW
                     PALIPLTMTLWAALEMGDDDIIAATRATITRDHGEAMADAMTWTRANTVRDMVTKGLP
                     EAWRRGPLLAEAAYPEVIVTRAVSDGAALALVLRAGAGPVRTRLGFARLVPGRRYRVV
                     QSGQEITAGADGTATLDFALDRRSLLDLVPVV"
BASE COUNT          384 a          832 c          765 g          287 t
ORIGIN      
        1 tattggccag aatgtcgagc ccgccgaagg cggccagcgc gcgggccacg gcggcctctg
       61 cggtggcggt ctgcgcgccg tctccggcca cggccaggca gcgcacgccg ctctcggcgc
      121 agatggcggc agcggccgcc cgcagcggcg cctcggcccg cgccaccagc accagatccg
      181 ccccccgcgc cgccagcagc cgggccacgg ccaggccaat cccccgcgag gcgccggtga
      241 cgatggcgct tttgccggca aaatcacgca tccccgctcc tttcagcgca gcgccaaagc
      301 cacgcgcacg cgttggccgg catccgcccg gccgccggtg cagacgggcg catacacgat
      361 cagctcgccc cggctgcggg ccagcgcgct atcggccagg cggtagagat gcgcgccatg
      421 gcgggtatag acccaccagc cgcccagccc gatttccagc gcgaacaggc aggccagccc
      481 cacccgcacc gccggccgcg ccagccagcc gcgcaggtgg ccaagcgccg tatccgcctc
      541 ttccccggcg cgtgggcgcg ccagccccca gaacaccgcc cagcgccgca aggtggccat
      601 cacatgagcg aacagcagcg cggccagcag gcagcaggcc atcgctagac caccggcacg
      661 agatcgagca ggctgcgcct gtcgagcgcg aaatcgagcg tggcggtgcc atcggcgcca
      721 gcggtgatct cctgcccgct ctgcaccacg cgatagcgcc ggccgggcac cagccgggca
      781 aagcccagcc gcgtgcgcac aggccccgcc cccgcccgca gcaccagcgc cagcgccgcg
      841 ccatcactca ccgcgcgggt gacgatcact tcgggatagg ccgcctcggc cagcagcggg
      901 ccccggcgcc aggcctcggg caggcccttt gtcaccatgt cgcgcacggt gttggcgcgg
      961 gtccaggtca tcgcatcggc catcgcctcg ccatgatcgc gcgtgatggt ggcgcgggtg
     1021 gcggcgatga tgtcgtcgtc gcccatttcc agcgcggccc acaaggtcat cgtcagcggg
     1081 atcagcgcgg gccactggtc gtaggagaaa ttggccggct tgcgcgtgtc ccagcccagc
     1141 ggcagcagct tgtagtccag cgtgccatcg ggcgcggggc ggatgaaatc ctgcttgagc
     1201 acctgccaca gccgctcggc ctgatcgggc gcgtgggggg tgaggaagcg gatgccccag
     1261 gtctcgccga acaggcccga caggctgggc acggtgaaac cgaaaggcga ggacgtgcac
     1321 accttgatgc ggccatcggc catcagcatt tcctcggcca tggtgcgctc gaagcgatcg
     1381 ccgatgcggg cgatcatatc gctgccatgg cgcgcatcat gcaacgccag cgcggtgcgg
     1441 cccaccagat tgcacatgga atagatccag cgcggctcgc acgggaacca gcccagatcg
     1501 tagcggcgga aattccggac cacctcttcc agcagcgttt cctggctgta ggcaaaggcg
     1561 gtgccctcat cccagcggaa ggtgagcgca ccgggggcgg caaagggcgc ggtgccgctc
     1621 gccgtctcga acaggcccag cgacacgccg agaaagcccg agagcatgat attgtcgatc
     1681 gccacggggt tcttctggat ctggaaattg ccccacaggc tttcccagaa ccagtagttc
     1741 cacgtcctgc ggtcgatatg cttggcgatg aggttgtgct gcgcctggtt gagatagccg
     1801 tggaaggcgg gcagctgcgt atattgcccg aaggccaggt tccacgacat ggtgcagatc
     1861 tgatagcgca gggcgccatc ctgccaggtg tcgttcatgg caaagccggt ccagctgtcc
     1921 accggctgca gcgccagatc gagcatgcgg cgaaattcgg ccacctgccc gtcgggcatt
     1981 tcgtcggcgg cggccacggg ccggtcgcgc aggatgggcg ccgtgcgggc gaggatctgg
     2041 ttcagctgcg ccccgcgcgc ctgctgggcg gcaaaatggc gccgccgctg caaggccagc
     2101 cagaggaaca ggccgatcac ccccgccggc agcccccagg ccaccgccgg cacgccgccg
     2161 cgcgccccca cccaggccgc agacagcagc gcgctgccca gcagcaccgc cggcgggcca
     2221 aggataaccc cgcgcgccca ccagatgaac aggatccagg caaaggcc
//
LOCUS       DASRYE010000048        14472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_19094,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000048 DASRYE010000000
VERSION     DASRYE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19094"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFF98_02810"
     CDS             <1..703
                     /locus_tag="VFF98_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZU62588.1"
                     /translation="AAAEAEHETPTILVKVDHHHAAADPVQQANRASFAAVQAADHVV
                     ISPLAQAYRVALPTPARDGVHNMLTNLREPVVISNCLLQHHFGRAGRSLARLALNTTL
                     GLGGLFDVARHKPFHLHHRANGFANTLGFYGMGAGPYFYLPIVGPTTLRDLAGTVADR
                     VFLSATVGWPFTRLEYRVPSTVLGTLDERLANDPQIRQLRAEKADFYAARRNAYLAQR
                     RAEIAALHRPETDIE"
     gene            809..2353
                     /locus_tag="VFF98_02815"
     CDS             809..2353
                     /locus_tag="VFF98_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62589.1"
                     /translation="MNKTTVKSFFEHHKDRFVLAFLYILIEALTNNPRVFVAARAAKW
                     ESWADQGSYFRSAHAFLQGNLDPAMHWYPLVYALILAPFSWLPPFYAALIPDLALIIG
                     CLVGFQMVAQRFGLSRWQALALFLPATLVYPHTGDSWLVPWTTTPSAAFIWLSLGLAF
                     RLEEGRRAPAEALGLGVLLGLIPLVRPGDALITAMVGLCLLKPLLIDARRWPELGAIC
                     LGGLAVILPYGALHVAIYGLHPSAYMALSGAYGLNLGRLGWKAYLIFIEPRPWYPYGD
                     GVLKRLPWFALGAAGLLVAACRKGRRWQVAVLALPALAYSAVMLAYIDLLPAGLWRFN
                     NIHYFKWIFLLLALFCWTFLRDLKHHPRLSLAAFLCVMLPAALRLEPVPATTGEPARL
                     AVFPAGVTEFADTYFSDIVLEDNAGHQVSSFDFHPVPDQAGLVLAEALRRDFAGNERL
                     RPRLPADHWPVEGAAIRNTARLPAVPQGAIARYRPALALGWPCWLPPYGCPASLPAAS
                     VPAPAP"
     gene            2520..4310
                     /locus_tag="VFF98_02820"
     CDS             2520..4310
                     /locus_tag="VFF98_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HZU62590.1"
                     /translation="MEYNCQTPDYATLIAEARALDTSACTRDLRIAVLADFTTHQLVA
                     VMQALAARRGVRLHVYEAAYDSIESEILDAASGLYAFAPDCVALLAATPKLKGRYYAA
                     ADPAAFAGQAVARFAGLWDGLRAHCPATIIQSTYVQPAERAFGQYEALVPHSLGAAVR
                     EVNRGLAEAARARRGVLLCDADFLAAERGRAAWRDERLWALAKLPCTPDCLPDLAGGV
                     LDAALAAAGAFVKCVVLDLDNTLWGGVIGDDGLAGIALGGFDEGEAFVAFQQFLLSLK
                     RRGILLAVVSKNEEAAARLPFREHPDMVLKEEDIAVFLANWDNKADNIRRVQQVLNIG
                     LDAMVFLDDNPFERQLVRAHVPGVIVPELPEDPVDYLPALAALNLFETASHSDADAAR
                     AGQYREEASRQQAQASFTNIDDYLRSLGMTARLERFAPRSLPRIAQLIQRSNQFNLTT
                     RRHSEEACARLMADAGHAPLTITLADTFGDYGLISVIVLALQGDTVEILDYLMSCRVL
                     KRGVEDLAMNAIFAFAAQNGARQVVGRYLPTAKNRMVEGFYAGFGFTCIDSAAHEAVW
                     AHEVASWRPRAHFIHAEAQEWGAAPQAVAG"
     gene            4325..4567
                     /locus_tag="VFF98_02825"
     CDS             4325..4567
                     /locus_tag="VFF98_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZU62591.1"
                     /translation="MPRDDIAARLTDLLRDFFRQPALALAEETTAADVPGWDSLAHID
                     LMVTIEKAFAIRLSTREVRGMKNIGALLDILAQKTA"
     gene            4592..5974
                     /locus_tag="VFF98_02830"
     CDS             4592..5974
                     /locus_tag="VFF98_02830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZU62592.1"
                     /translation="MGFTDFGFWLALLPVVALFHALPGRWRPAWLLAASLAYFTIESG
                     AYVLLLLASVGWVWAAGHRIARAETDRARLIWLWAGLVPAAGVLVLFKLGDAMPGIAA
                     PLGISYYTFRLIAYLVEVYWDERQLAPGWLDFAAYVSFGPHMVSGPIQRPGDFLPQLA
                     ALRHRLDPARIEGGLQMLLNGLMLKLIIGDRLAPLVNMVDGAPAAYARPAVWLAAVAH
                     LPYLYADFAGYTLIALGLGALFGIAGPPNFNAPFAATNVAEYWRRWHMSLTGWLTDYV
                     FMPLRMATRHWGRAGLVLSIFVNMVLVGLWHRISGTYLVFGLVQGAYVAVYTLWLAPR
                     GGPARGTGQPLVALAKAVCVFVLMDAALIVWFAPALPSAATEARMVLGLLPPGPRLFE
                     ELPEGADIKAACIALVLAIYIGAGAPGMAAIRARLGRHVPGWAWVALKLLLISLLGQD
                     NGGAFIYGHF"
     gene            5961..7121
                     /locus_tag="VFF98_02835"
     CDS             5961..7121
                     /locus_tag="VFF98_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62593.1"
                     /translation="MAISEARPWRTFLLGLLLFWAVDTAIFGSGWYGPHQSPILKTGD
                     FWFTMTDVQQRPADPARDVLVLGHSRMGEGFSFAQVQAEHLGGGLHFVSAIMPGSDFK
                     WWYFQLRALDPAGNRYRAVVIPVNGYLARPDASSMKVAADKGVGPALRPFLALRDWPA
                     FIASFDTPGERASALAVLLFPGHSLGVDAVSWLRSPGGRRAEIASRLALGLKARHEYQ
                     GYGAEDLSGLRFDYASCAVSAWPAQFDLFRQKDSQKYLCRPADPAGETRMAEAYNRYW
                     LQRIADLYRGTHTRVIVVPVPAYGVAMAGVDPIAGAPDIRTGLADAPQSPLLFLPEAP
                     FAALARRANFYDLVHMNRRGRTLFTRALAGAVAQALPGTTPPPPAAPPRPAP"
     gene            complement(7118..7777)
                     /locus_tag="VFF98_02840"
     CDS             complement(7118..7777)
                     /locus_tag="VFF98_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU62594.1"
                     /translation="MTNGATVYLIDDDASLRNSIRFLLASHGRDVIGFPTGEAFLDRQ
                     PGLEPGPILLDVQMTGMDGLMVQRLLLRQGCTWPVIILTGHGNIAMAVRAIKSGAVDF
                     LTKPFARADLLAVLEQADARLADQDAMHNQQEVARHRLCALTVREQQVLNELARGLPN
                     KTIAYDLGISSRTVEIYRANLMRKLGVQSFPDALRIAFAAGMPFAAGKREVAVAWSAL
                     G"
     gene            complement(7774..8967)
                     /locus_tag="VFF98_02845"
     CDS             complement(7774..8967)
                     /locus_tag="VFF98_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU62595.1"
                     /translation="MERGATRDGTASRAKRRKVHLPKLSAAARGDFLRSLMAAFPDAM
                     VLADETGRIVAFSDSEKAMLGYSEADIIGQNIAMLMPSPERQQHGSFIRKYLDTGEKR
                     AIGFDNLVRVQRKNGEIIPVDLSLHEAQVDGKRMFIGFMHNASVREEQRQRLTQLSAE
                     LAHASRMSSMGLLSSAIAHELNQPLTAIRNYVETLAAILQGETAINRATLAEVMRACD
                     HEATRAGEIIRRLRQFISRGETEHTRESLKALVSDAVMLALADGEGIDIRLDIAIAAA
                     TDEVLVDGIQIQQVIFNLVRNALQAMAERPERRLRITSCAHEDMVEVAIEDNGSGIAP
                     ELERKLFSPFTTTKIDGLGLGLSIVKMIVEAHGGRIWVAPSSLGGCGFHFTIPAMQSI
                     REGAT"
     gene            9268..9642
                     /locus_tag="VFF98_02850"
     CDS             9268..9642
                     /locus_tag="VFF98_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62596.1"
                     /translation="MRISYALIAAAGLIVGGTAHADSTSLNAGSISVTAVGSTSSTSC
                     SGGHCGALAGGGAAGGGLAGSSASSTTGHHGTTDTTTAGSFGGVVQGSGSTTSGHGGS
                     ASSSSGGIGIYGAGATASTHSH"
     gene            9829..10875
                     /locus_tag="VFF98_02855"
     CDS             9829..10875
                     /locus_tag="VFF98_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014500277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZU62597.1"
                     /translation="MNRLWTFASTIALVAGSISPAAAQTSTSTSGSNSGAVSGSNSAS
                     SSNPVNTTTTTTNSTSASGASSTSATNSTASNQGNSQTINNNSNYPANQTVKMAPALV
                     APALTTTLTETCMGSTSLGVSVLGFGATGGTTWQDHQCARRLNARELAQTMGDRDAAR
                     ELMCTDEEVFRVYNALGRPCRLKPDGTPNPAYMPPPPPPPAAAPAPVAAMPGPFLVFF
                     DWDKADITPEASATLDKAASAYQQTGMASVQLTGHTDKTGAADYNMALSLRRAESVKV
                     YLAGRGVPRDVMVVHGMGATQPRVQTADGVREVQNRRVEIAFVGPGSAPDTNAPNGDG
                     TPPTEGSGSAKASN"
     gene            complement(11005..12990)
                     /locus_tag="VFF98_02860"
     CDS             complement(11005..12990)
                     /locus_tag="VFF98_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU62598.1"
                     /translation="MSALSDLLLPVARMLDLYRLRTDDPELLRAQFRAFVRNLPLLYF
                     MLACNAMAVVVAYAPFAHPRWCDLFAGFLVLVAGMRGVWWWRRRGVVFTDAQIRRHIR
                     NTNALAGFMAMAFMVWALQLYPYGDAYARGHLVLFLALTQLGSVAGLMPVRSAALAVA
                     TVAMVPFTAYFLFADGGRMAVDAINFGLVGSAILVLFHVQNEAFAALVQSRRDLHLRQ
                     LETQKLSDDNRRIAFTDALSGLPNRRALIARLEDVREGEVAMPDRLAVIFIDLDGFKD
                     INDNYGHEFGDALIRRMGERLRSVCAPGVMLARMGGDEFAVLIAREGAEAFARATADR
                     ILSTLTVPLDIAGKACHVGASIGLACDADGTASPSELLRRADTAMYRVKAEGKGGVLG
                     YDPSFDADRLRRQSIEREIRAGLERGEFSVVYQPLVDAVSGAMVGVEALLRWPGRPGG
                     PLGPDDFIDIAEASGLIQPLGQFVLRRACEEMAGLAPLSLSVNISPAQFRYPGFERGV
                     ADVLVDTGFDPARLQIEITEGYLIDNPARAARAIDAFREMGVGVALDDFGTGFASIGY
                     LQAYGFTCIKIDKTLCANLGGDPRTGMLISGLVFMAKGLDLKVVAEGVETEAQAALLR
                     LAGCHTLQGYLFGRPAPLADLAPWRGRAGQGWQVPGS"
     gene            13267..14121
                     /locus_tag="VFF98_02865"
     CDS             13267..14121
                     /locus_tag="VFF98_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HZU62599.1"
                     /translation="MANPILRQKLASGQFFVVPGLQDMVAAAIANKVGFDIVYGTGYW
                     LTASAWGLPDAGIATYTQMLNRMETLVKTSHGAVIADGDTGYGGLLNVHHTVQGYEAA
                     GVTCIQLEDQEFPKKCGHTPFKRLIPLEDMVDKIKVAVDARQSEDFLIIARTDARQSE
                     GMDGVLRRLEAYDKAGADIIFPEALTSEEEMARACAAFAKPVMANMANGGLTPVPHAS
                     VLAEIGYGFAIYPSLTSLVAATAMEKTLRALKDEGIGEPDELFNFKEFCELIGFDKVW
                     EFERKWAR"
     gene            14262..>14472
                     /locus_tag="VFF98_02870"
     CDS             14262..>14472
                     /locus_tag="VFF98_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62600.1"
                     /translation="MPPPPPPADADAQRTQLEARLAAALIYCGRTYRRQVDRHLAAHQ
                     LSDARALPVMQISRAGNALRQRDLAE"
BASE COUNT         2358 a         5045 c         4571 g         2498 t
ORIGIN      
        1 cgccgccgca gaggccgagc atgaaacgcc gacgatcctc gtcaaggtgg atcatcatca
       61 cgccgcggca gatccggtgc agcaggccaa ccgtgcctcc tttgccgcgg tgcaggcggc
      121 cgatcatgtg gtgatctcgc cgctggccca ggcctatcgc gtcgccctgc ccacacccgc
      181 gcgcgatggg gtgcacaata tgctcaccaa cctgcgcgag ccggtggtga tctccaattg
      241 cctgttgcag catcatttcg gccgggcggg ccgatcgctg gcgcggctgg cgctgaacac
      301 cacgctgggc ctgggcggcc tgttcgacgt ggcccggcac aagcccttcc acctgcacca
      361 cagggccaac ggctttgcca acacgctggg cttttatggc atgggcgcgg ggccctattt
      421 ctatttgccg atcgtggggc cgaccaccct gcgcgatctg gccggcaccg tggccgaccg
      481 ggtgttcctt tccgcaacgg tgggctggcc gttcacccgg ctggaatatc gcgtgccttc
      541 caccgtgctc ggcacgctcg acgaacgcct cgccaacgat ccccagatcc gccagctgcg
      601 cgcggaaaag gccgatttct atgcggccag gcgcaacgcc tatctggccc agcgccgtgc
      661 cgaaatcgcc gccctgcacc ggcccgaaac cgacatcgaa taggcccggt cggccagcct
      721 cagccaattg atctttccac ccaaatctgt agtgagcgca aaattgggcc acccctttgc
      781 gcccacttgc gaaccccggg cagcgacgat gaacaaaacc accgtgaaat ccttctttga
      841 acaccacaag gacagatttg ttctggcgtt tttgtatatc ctcatcgaag cgctcaccaa
      901 caatccgcgg gtattcgttg ccgcccgcgc cgccaaatgg gaaagctggg ccgatcaggg
      961 cagctatttc agatcggccc acgcctttct tcaaggcaac cttgatccgg ccatgcattg
     1021 gtatccgctg gtctatgcgt tgatcctggc gccgttctcc tggctgccgc cgttctatgc
     1081 cgcgctgatc ccggatctgg cgctcatcat cggctgcctc gttggcttcc aaatggtggc
     1141 gcagcgcttt ggcctgtcgc gctggcaggc gctggcgttg tttctgccgg ccacgctggt
     1201 ctatccgcac accggcgatt cctggcttgt gccgtggacg acgacccctt cggcggcctt
     1261 catctggctg tcgctgggcc tggccttccg gctggaggaa gggcgccggg cgccggcaga
     1321 agcgctgggg ctgggcgtgt tgctgggcct gatcccgctg gtgcgcccgg gcgatgcgct
     1381 gatcacggca atggtaggcc tgtgcctgct gaagccgctg ctgatcgatg cgcgccgctg
     1441 gccggaactg ggggcgatct gcctgggcgg gctggccgtt atcctgccct atggcgcact
     1501 gcatgtggcg atctatggcc tgcacccttc cgcttatatg gctttgtcgg gcgcttatgg
     1561 gctgaacctg ggccggctgg gctggaaggc ctatctcatc ttcatcgagc cgcgcccgtg
     1621 gtatccttat ggcgatggcg tgctgaagcg gctgccgtgg tttgcgctgg gcgcggccgg
     1681 gctgctggtg gcggcctgcc ggaaggggcg gcgctggcag gtggcggtgc tggcgctgcc
     1741 ggcgctggcc tacagcgcgg tcatgctggc ctatatcgac ctgttgccgg caggcctgtg
     1801 gcgcttcaac aacatccatt atttcaagtg gatcttcctg ctgctggcgc tcttctgctg
     1861 gaccttcctg cgtgatctga agcaccatcc gcgcctcagc ctggccgcct tcctgtgtgt
     1921 gatgctgccc gccgcgctgc ggctggagcc ggtgccggcc accactggcg agcccgcccg
     1981 gctggcggta ttccccgctg gcgtcaccga atttgccgac acctatttca gcgacatcgt
     2041 gctggaagac aacgccggcc atcaggtcag cagcttcgat ttccaccccg tgcccgatca
     2101 ggccgggctg gtgctggccg aagcgctgcg ccgcgatttt gccggcaacg agcgcctgcg
     2161 cccccgcctg ccggccgacc actggccggt ggagggcgcc gccattcgca acaccgcccg
     2221 cttgcccgcc gtgccgcagg gcgccatcgc ccgctatcgg ccggccctgg cgctgggctg
     2281 gccctgctgg ctgccgccct atggctgccc ggccagcctg cccgcagcgt ccgttccggc
     2341 ccccgcgccc tgattgcgac actaaattac gcaattccac gatcttgcaa cctcgcctca
     2401 atctcgtgaa atctggcggc aacacggcaa tgtcctagcc ctgttctggc tgcgtctgcc
     2461 ccttgccggg ccgggcgatc atccggtccg cccagacaag cgcagagcga ggcatcatcg
     2521 tggagtacaa ctgccagacg ccggactatg ccacgctgat cgccgaggcg cgcgcgctgg
     2581 acaccagcgc ctgcacccgc gacctgcgca ttgccgtgct ggccgatttc accacccatc
     2641 agctggtggc ggtgatgcag gcgctggccg cgcgcagggg cgtcaggctg catgtgtatg
     2701 aggctgctta cgattcgatc gagagcgaga tcctcgatgc cgcctccggc ctctatgcct
     2761 tcgcgcctga ttgcgtggcg ctgctggccg ccacgcccaa gctgaaaggc cgctactatg
     2821 ccgcggccga tccggcggcc tttgccgggc aggcggtggc gcgctttgcc ggcctgtggg
     2881 atgggctgcg cgcccattgc ccggccacca tcatccaatc cacctatgtg cagccggccg
     2941 agcgggcgtt tggccagtat gaagcgctgg tgccccacag cctgggcgcg gccgtgcgcg
     3001 aggtgaacag gggcctcgcc gaagcggccc gggcgcggcg cggcgtgctg ctgtgcgatg
     3061 ccgatttcct cgccgccgaa aggggccgcg cggcctggcg cgacgagcgg ctgtgggcgc
     3121 tggccaagct gccctgcacg cccgattgcc tgcccgatct ggccggcggc gtgctggatg
     3181 cggcgctggc ggcggcgggc gctttcgtca aatgcgtggt gctcgatctc gacaacacgc
     3241 tgtggggcgg ggtgatcggc gatgatgggc tggccggcat cgcgctgggc ggtttcgacg
     3301 agggtgaggc tttcgtcgcc ttccagcagt tcctgctgtc gctgaagcgg cgcgggatcc
     3361 tgctggccgt ggtcagcaag aacgaggaag cggccgcccg cctgcccttc cgcgaacacc
     3421 ccgacatggt tctgaaagag gaggacatcg ccgtcttcct cgccaactgg gacaacaagg
     3481 ccgacaatat ccgccgcgtg cagcaggtgc tgaacatcgg cctcgatgcg atggtcttcc
     3541 tcgacgacaa ccctttcgag cgccagctgg tgcgcgccca tgtgcccggc gtgatcgtgc
     3601 ccgaactgcc ggaagatccg gtggattacc tgcccgcgct ggccgccctc aacctgttcg
     3661 agaccgcctc ccattccgat gccgatgccg cccgcgccgg ccagtatcgc gaggaagcct
     3721 cgcgccagca ggcccaggcc agcttcacca atatcgacga ctacctgcgc tcgctgggga
     3781 tgaccgcccg gctggaacgc tttgcccccc gcagcctgcc gcgcatcgcc cagctgatcc
     3841 agcgctccaa ccagttcaac ctcaccaccc gccgccacag cgaggaagcc tgcgcccggc
     3901 tgatggccga tgccggccac gcccccctca ccatcaccct ggccgacact ttcggcgatt
     3961 atgggctgat ctcggtgatc gtgctggcgc tgcagggcga tacggtggaa atcctcgact
     4021 atctgatgag ctgccgggtg ctgaagcgcg gcgtggaaga tctggcgatg aacgcgatct
     4081 ttgcctttgc cgcgcaaaac ggcgcgcgcc aggtggtggg ccgctatctc cccacggcca
     4141 agaaccgcat ggtggaaggc ttctatgccg gcttcggctt cacctgcatc gacagcgccg
     4201 cgcatgaggc ggtgtgggcg catgaagtgg ccagctggcg cccgcgcgcc catttcatcc
     4261 acgccgaggc ccaggaatgg ggcgcagcgc cgcaagctgt ggccggctga caggagacat
     4321 gcccatgccc cgtgacgaca tcgccgcccg gctgaccgac ctgctgcgcg acttcttccg
     4381 ccagccagcc ctcgccctcg ccgaggaaac caccgcggcc gatgtgcccg gctgggattc
     4441 gctggcccat atcgacctta tggtgacgat cgagaaggcc tttgccatca ggctttccac
     4501 ccgcgaggtg cgcggcatga agaacatcgg cgcgctgctc gatatcctcg cccagaaaac
     4561 cgcctgatcg cgccggagcc agacaccgct catgggcttt accgatttcg gcttctggct
     4621 ggcgctgctg ccggtggtgg cgctgttcca tgcgctgccc gggcgctggc ggccggcctg
     4681 gctgctggcg gcatcgctgg cctatttcac catcgagagc ggcgcctatg tgctgctgct
     4741 gctggcctct gtgggctggg tgtgggcggc aggccaccgc atcgcccgcg ccgaaacaga
     4801 cagggccagg ctgatctggc tgtgggccgg cctggtgccg gcggccggcg tgctggtgct
     4861 gttcaagctg ggcgatgcca tgccggggat cgcggcgccc ctgggcatct cctattacac
     4921 cttccggctg atcgcctatc tcgtcgaggt ctattgggat gagcgccagc tggcgccggg
     4981 ctggctcgat tttgccgcct atgtcagctt tggcccgcat atggtgagcg ggccgatcca
     5041 gcgcccgggg gatttcctgc cgcagctggc ggccctgcgc cacaggctgg atccggcccg
     5101 catcgagggg ggcttgcaga tgctgctgaa cgggctgatg ctcaagctca tcatcggcga
     5161 tcggctggcc ccgctggtca acatggtgga tggcgcccct gccgcctatg cccgcccggc
     5221 ggtgtggctg gcagcggtgg cgcatctgcc ctatctctat gccgattttg ccggctacac
     5281 gctgatcgcg ctgggccttg gcgcgctgtt cggcatcgcc gggccgccca atttcaacgc
     5341 ccccttcgcc gccaccaacg tggccgaata ctggcgccgc tggcacatga gcctgaccgg
     5401 ctggctgacc gattatgtgt tcatgccgct gcgcatggcc acacgccact ggggccgggc
     5461 ggggctggtg ctgtcgatct ttgtcaacat ggtgctggtg gggctgtggc accggatcag
     5521 cggcacctat ctcgtgtttg gcctggtgca gggcgcctat gtcgcggtct acacgctgtg
     5581 gctggcgccg cgcggggggc cggccagagg caccggccag ccgctggtgg cgctggccaa
     5641 ggcggtgtgc gtcttcgtgc tgatggatgc ggcgctgatc gtgtggtttg ccccggccct
     5701 gccctcggcc gccaccgagg cgcgcatggt gctgggcctg ctgccgcccg gcccccgcct
     5761 gttcgaggaa ttgcccgaag gggcagacat caaggccgcc tgcatcgcgc tggtgctggc
     5821 gatctacatc ggcgccggcg cgccgggcat ggcggccatc cgcgcgcgcc ttggccgcca
     5881 tgtgccgggc tgggcctggg tggcgctgaa gctgctgctg atctcgctgc tggggcagga
     5941 taatgggggg gctttcatct atggccattt ctgaggcccg gccctggcgc acgttcctgc
     6001 tgggcctgct gctgttctgg gcggtggata cagcgatctt cggctcgggc tggtatggcc
     6061 cgcaccagtc gcccatcctc aagacgggcg acttctggtt caccatgacc gatgtgcagc
     6121 agcgcccggc cgatcctgcc cgcgatgtgc tggtgctggg ccactcgcgc atgggcgaag
     6181 ggttctcctt cgcgcaggtg caggccgagc atctgggcgg cggcctgcat ttcgtctcgg
     6241 cgatcatgcc gggcagcgat ttcaaatggt ggtatttcca gctgcgcgcg ctcgatccgg
     6301 cgggtaaccg ctatcgcgcg gtggtgatcc cggtgaacgg ctatctggcc cggcccgatg
     6361 ccagttcgat gaaagtggcg gccgacaagg gcgtggggcc agccctgcgc cccttcctgg
     6421 ccctgcgcga ctggcccgct ttcatcgcct ccttcgacac gccgggcgaa cgggcgagcg
     6481 cgctggcggt gctgctgttt cccggccaca gcctgggggt ggatgcggtg agctggctgc
     6541 gctcgccagg cgggcgccgg gccgagatcg ccagccggct ggcgctgggc ctgaaagccc
     6601 gccacgaata tcaaggctat ggcgcagaag acctgagcgg cctgcgcttc gattatgcca
     6661 gctgcgcggt cagcgcctgg ccggcgcagt tcgacctgtt ccgccagaag gattcgcaga
     6721 aatatctgtg ccgcccggcc gatcccgctg gcgaaacccg catggccgag gcttataacc
     6781 gctattggct gcagcgcatc gccgatcttt atcgcggcac gcacacgcgg gtgatcgtgg
     6841 tgccggtgcc ggcctatggc gtggcgatgg cgggcgtgga tccgattgcc ggcgcgcccg
     6901 atatccgcac cgggctggct gatgcaccac aatcgccgct gctattcctg cccgaggcgc
     6961 cctttgccgc cctcgcccgg cgcgccaatt tctacgatct ggtgcatatg aaccggcgcg
     7021 ggcgcacgct gttcacccgg gcgctggcgg gcgctgtggc ccaggccctg cccggcacca
     7081 ccccgccgcc acccgccgcc ccgccacggc ccgcccctta acccagcgcg ctccacgcca
     7141 ccgccacttc gcgcttgccg gcggcaaacg gcatgccggc ggcaaaggcg atgcgcagcg
     7201 catcgggaaa ggattgcacc cccagcttgc gcatcagatt ggcgcgatag atctccaccg
     7261 tgcgcgagct gatgcccaga tcataggcga tggtcttgtt gggcaggccc cgggccagct
     7321 cgttcagcac ctgctgttcg cgcacggtca gggcgcacag gcggtggcgc gccacttcct
     7381 gctggttatg catggcatcc tgatcggcca gccgggcatc ggcctgttcc agcaccgcca
     7441 gcagatcggc ccgggcaaag ggcttggtga ggaaatccac cgccccgctc ttgatcgcgc
     7501 gcaccgccat ggcgatgttg ccgtgcccgg tgaggatgat caccggccag gtgcagccct
     7561 ggcgcagcag caggcgctgc accatcagcc cgtccatccc ggtcatctgc acatccagca
     7621 ggatcggccc cggctccagc cctggctggc ggtcgaggaa ggcctcgccc gtggggaagc
     7681 cgatcacatc gcggccatgg ctggccagaa gaaaacggat cgaattgcgc aggcttgcgt
     7741 cgtcatcgat cagataaacc gtcgcaccat ttgtcatgtc gcgccctccc ttatcgattg
     7801 catcgccgga atggtgaagt gaaaaccgca tccacccaaa gaggaaggcg caacccatat
     7861 ccttccgcca tgggcttcca cgatcatctt cacaatcgat agccccaatc ccaaaccatc
     7921 tatctttgtt gtcgtgaatg gcgagaacag cttcctttcc agttctgggg cgatgccgga
     7981 gccattatcc tctatcgcca cttccaccat atcttcatgt gcgcagctgg tgatgcgcag
     8041 gcgccgctcc ggccgttcgg ccatggcctg cagggcattg cgcaccagat tgaaaatcac
     8101 ctgctggatc tggatgccat cgaccagcac ctcgtcggtg gccgcggcaa tggcgatgtc
     8161 gagccggata tcaatgccct cgccatcggc cagcgccagc atcaccgcat cgctcaccag
     8221 ggccttcagc gattcgcgcg tgtgttcggt ttccccgcgc gagatgaact ggcgcagccg
     8281 gcggatgatc tcgccggcgc gcgtggcctc atggtcgcag gcgcgcatca cctcggccag
     8341 cgtcgcccgg ttgatcgccg tttcgccctg caggatggcc gccagcgttt ccacatagtt
     8401 gcggatggcg gtgagcggct ggttcagttc atgggcaatc gcgctcgaca gcagccccat
     8461 cgacgacatg cgcgaggcat gcgccagttc ggccgaaagc tgggtcaggc gctggcgctg
     8521 ctcctcgcgc acgctggcgt tgtgcatgaa gccgatgaac atgcgtttac catccacctg
     8581 cgcttcgtga agcgacaggt cgaccgggat gatctcgccg ttcttgcgct gcacgcgcac
     8641 cagattatcg aaaccgatgg cgcgtttttc gcccgtatcc aaatatttcc gaataaagga
     8701 tccgtgctgc tggcgttctg gcgaaggcat cagcatggca atattctggc caattatatc
     8761 cgcttcgcta tagcccagca tggctttttc ggaatcgctg aaggcaacga tccggccggt
     8821 ttcgtcagcc agcaccatcg catcgggaaa ggccgccatc agcgagcgca gaaaatcccc
     8881 gcgcgctgcc gcagacagct tcggcaggtg taccttgcgc ctcttcgcac gagacgcagt
     8941 accgtccctc gtcgcccctc tctccatacg ggacaattac catcccctct ctggccgtgg
     9001 ataggttatt atacttatcc atggcggcgc cgccccattc tgaggcaatc gcccgagctc
     9061 cccgccggca tcatacgcaa tatgccgtat gggtacaaac gtataaaaaa gccgcccccg
     9121 ctaactatgt tgaaacccgt agatcgctcc tcgttggagt cgatcctacg aatagcgggg
     9181 cttcctcaca attctattcc cccttcggga atgatgcgga ccatgcgtca tccgcaatcc
     9241 ctgacgcaca accaaggaga gcatgccatg cgtatttcct atgcactcat cgcggctgcc
     9301 gggctgattg tcggtggcac ggcccatgcc gattccacat cgctgaatgc cggctcgatt
     9361 tcggtgacgg ccgtcggctc gaccagctcc acttcctgct cgggcggcca ctgcggcgcg
     9421 ctggcgggcg gcggcgcggc gggcggcggc ctggccggtt cctcggcctc gtccaccacc
     9481 ggccaccacg gcaccaccga caccaccacc gcgggcagct tcggcggcgt cgtccagggc
     9541 agcggttcga ccacttcggg ccatggcggc agcgccagca gcagcagcgg cgggattggc
     9601 atctatggtg ccggcgccac cgccagcacg cactcgcact gagccggccg gcggtcgcag
     9661 gggagggccg ccccctcccc tcaccgctca gcggcccgtg ccgccgcttc aggctccgcg
     9721 ctttctcccg tccgcgccgc ctgatctctg gctcgcccct tattccacca ggccccgatc
     9781 gcggcccgtg acggcgcgct cgggaaaatc ctagtcagga ggattgccat gaacaggctt
     9841 tggacatttg ccagcaccat agcgctggtg gccggcagca tcagcccggc cgcagcgcaa
     9901 accagcacct cgacctcagg ctcgaattcg ggtgcggttt cgggttccaa ctcggcttcc
     9961 tcgtcgaacc ccgtcaacac gacgacgacg acgaccaact ccacctcggc ttcgggcgcc
    10021 agttcgacat cggccaccaa ttcgaccgcg tcgaaccagg gcaactcgca gacgatcaac
    10081 aacaattcga actatccggc caaccagacg gtgaagatgg cccccgccct ggtggcgccg
    10141 gcgctgacca ccaccctcac cgaaacctgc atggggtcca cctcgctggg ggtctcggtg
    10201 ctgggctttg gcgccaccgg cggcaccacc tggcaggatc accagtgcgc ccgccgcctc
    10261 aacgcgcgcg aactggccca gacgatgggc gaccgcgatg ccgcgcgcga gctgatgtgc
    10321 accgacgagg aagtgttccg cgtctataac gccctgggcc gcccctgccg gctgaagccc
    10381 gatggcacgc ccaacccggc ctatatgccg ccgcccccgc cgccgccggc ggcagcgccc
    10441 gcacctgtgg ccgccatgcc cgggcccttc ctcgtcttct tcgactggga caaggccgat
    10501 atcacgcccg aggcctctgc cacgctcgac aaggcggcca gcgcctatca gcagaccggc
    10561 atggccagcg tgcagctcac cggccacacc gacaagacag gcgctgccga ctacaacatg
    10621 gcgctctcgc tgcgccgggc ggaatcggtg aaggtctatc tggccggccg gggtgtgccc
    10681 cgcgatgtca tggtggtgca cggcatgggc gctacccagc cgcgcgtgca gaccgccgat
    10741 ggcgtgcgcg aggtgcagaa ccgccgggtg gagatcgcct ttgtcgggcc tggcagcgca
    10801 cctgacacca acgcgcccaa cggggatggc acccccccga cagaaggcag cggcagcgct
    10861 aaggccagca actaggcggc tgcgcccgag cccccccgag cggccgccga ccggaaagga
    10921 gccatcctca caccatggcc cctttccggt ttttttgcgt gaaccgcaag cccggctttc
    10981 tggcgtgccc gcaagccggc attctcagct gccgggcacc tgccagccct gccccgcccg
    11041 gccgcgccag ggcgccagat cggccagcgg cgccggccgg ccaaacagat agccctgcag
    11101 cgtgtggcag ccggccagcc gcagcagggc ggcctgggcc tctgtttcca ccccttcggc
    11161 caccactttc agatccagcc ccttggccat gaacaccagc cccgagatca gcatgcccgt
    11221 gcgcggatcg ccgcccagat tggcgcacag cgtcttgtcg atcttgatgc aggtgaagcc
    11281 ataggcctgc agatagccga tgctggcaaa gccggtgcca aaatcatcca gcgccacgcc
    11341 cacgcccatt tcgcggaagg catcgatggc gcgcgcggca cgggcggggt tgtcgatgag
    11401 atagccctcg gtgatctcga tctgcaggcg ggccggatca aagcctgtat ccaccagcac
    11461 atcggccacg ccgcgctcga agccgggata gcggaactgg gcgggcgaga tgttgacgct
    11521 gagcgagagc ggggcaaggc cggccatctc ctcgcaggcg cggcgcagca caaactggcc
    11581 cagcggctgg atcaggccgc tcgcctcggc aatatcgatg aaatcatccg ggcccagcgg
    11641 gccgcccggc cgccccggcc agcgcagcag cgcctccacc cccaccatcg ccccgctcac
    11701 cgcatcgacc agcggctgat agacgacgga gaactcgccg cgttccagcc cggcgcggat
    11761 ctcgcgctcg atcgactggc ggcgcaggcg gtcggcatcg aaggagggat catagcccag
    11821 cacgcccccc ttgccctcgg ccttcacgcg gtacatcgcc gtatcggccc ggcgcagcag
    11881 ttccgatggg ctggccgtgc catcggcatc gcaggcaagg ccgatgctgg cccccacatg
    11941 gcaggccttg ccggcaatat cgagcggcac cgtcagcgtg gaaaggatgc gatcggcggt
    12001 ggcgcgggca aaggcctcgg cgccttcgcg ggcgatcagc acggcaaatt catcgccccc
    12061 catgcgcgcc agcatcacgc cgggcgcaca tacggaacgc agccgctcgc ccatccggcg
    12121 gatcagcgca tcgccaaatt catgcccgta attgtcgttg atgtccttga agccgtcgag
    12181 atcgatgaag atcaccgcca gccggtcggg catcgccacc tcgccttcgc gcacatcctc
    12241 cagccgggcg atcagcgcgc gccggttggg caggcccgac agcgcatcgg taaaggcgat
    12301 gcggcggttg tcgtccgaca gcttctgcgt ttccagctgg cgcaggtgca gatcccggcg
    12361 cgattgcacc agcgccgcaa aggcctcgtt ctgcacatgg aagagcacca ggatggcgct
    12421 gcccaccagg ccgaaattga tcgcatccac cgccatgcgg ccgccatcgg caaacaggaa
    12481 ataggcggtg aagggcacca tcgccaccgt ggcgaccgcc agcgcggcgg aacgcaccgg
    12541 catcagcccg gccacgctgc ccagctgcgt caacgccaga aacagcacca gatggccgcg
    12601 cgcataggca tcgccataag gataaagctg cagcgcccac accatgaagg ccatcgccat
    12661 aaacccggcc agcgcattgg tgttgcggat atggcggcgg atctgcgcat cggtgaacac
    12721 caccccgcgc cggcgccacc accacacccc gcgcatgccc gccaccagaa ccagaaaacc
    12781 ggcgaacaga tcgcaccagc gcggatgggc aaagggcgca taggccacca ccaccgccat
    12841 cgcattgcag gccagcatga aatagagcag cggcagattg cgcacaaaag cgcggaactg
    12901 cgccctcagc agttcagggt catcggtgcg caggcggtag agatcgagca tccgcgcaac
    12961 cggcaacagc agatccgaca aagcgctcac ttgcaactca cacagcctcg gggcgagtgc
    13021 ttatccgaca acgccggtaa gattgtctta atccgccccc agcagctctg cgcagaaagg
    13081 cgtaaacccc ttggcaagcg gcccctcgcc cgccctgccc caaaggcggg tggcggggca
    13141 aattgcatgc attggcaagg gcgaaaccct gaaaatacgc cgccacatgc gaagacaccc
    13201 cttttaatgt atacaaatac gcgttaagcg gcagaccaca tcccttccca gcaaaggttc
    13261 cctgccatgg ccaaccccat tctgcgtcaa aagcttgcct ccggtcagtt cttcgtcgtg
    13321 cccggccttc aggacatggt cgccgctgcc atcgccaaca aggtcggctt cgatatcgtc
    13381 tatggcaccg gttattggtt gacagccagc gcctggggcc tgcccgatgc cggcatcgcc
    13441 acctacacgc agatgctcaa ccgcatggaa acgctggtga aaaccagcca tggcgcggtt
    13501 atcgccgatg gcgacaccgg ctatggcggg ctgctcaacg tgcatcacac ggtgcagggc
    13561 tatgaagccg ccggcgtcac ctgcatccag ctggaagacc aggaatttcc caagaaatgc
    13621 ggccacaccc cgttcaagcg gctgatcccg ctggaagaca tggtggacaa gatcaaggtg
    13681 gcggtggacg cgcgccagag cgaggatttc ctcatcatcg cgcgcaccga tgcccgccag
    13741 agcgagggca tggacggcgt gctgcgccgc ctggaagcct atgacaaggc cggcgccgac
    13801 atcatcttcc ccgaagcgct gaccagcgag gaggaaatgg cccgcgcctg cgccgccttt
    13861 gccaagccgg tgatggccaa catggccaat ggcggcctta cccccgtgcc ccatgccagc
    13921 gtgctggccg agatcggcta tggctttgcc atctacccct cgctgaccag cctggtggcc
    13981 gccaccgcca tggagaaaac cctgcgcgca ctgaaggacg agggcattgg cgagcccgac
    14041 gagctgttca acttcaagga gttctgcgag ctgatcggct tcgacaaagt gtgggaattc
    14101 gaacgcaagt gggcccgtta aggcgccttt cgccaaggcg gccggcatgc gcggggacag
    14161 gccggccgcc ttttttgctg cactgcaaaa taatcccgga cacacccctt gcgccgatat
    14221 agatagcacg ctatcgaatt tgcgtaggac gctatcgaat catgcccccc ccgccccccc
    14281 ctgccgatgc ggatgcccag cgcacccaac tggaagcacg ccttgccgcc gcgctgatct
    14341 attgcgggcg cacctatcgc cggcaggtgg acaggcatct ggccgcccat cagctgtcgg
    14401 atgcacgggc gctgccggtg atgcagatct cgcgggcggg caatgcgctg cgccagcgcg
    14461 atctggccga gc
//
LOCUS       DASRYE010000049         9761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_215002,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000049 DASRYE010000000
VERSION     DASRYE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215002"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFF98_02875"
     CDS             complement(<1..931)
                     /locus_tag="VFF98_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62601.1"
                     /translation="MQAQFDAASDAWGRGDCAGALPIYARLAADARIQPGSLPQAAIM
                     VRRGECLVRTGHAPEGEPLLAAGLPRLRAAGADFQAEVVDGENMLGVLAMARWDHDTA
                     LAHFQAALRLEQGIDRLPELMEIAQVTAFDGGTQALAAVEEGLRLSEAKPVPDRREIA
                     QWHWQRAKVLLNQGQAAQARAELLQAQALNGGEKAVVGIADSALFADLAGASMRLQNR
                     NGDAYRYMAASGAGRLANTPFARPVFMEPPACGPDTGLEPEDVAVVEFVIGDDGQVQA
                     AQTVTTAGTYAKAATFARAVSQWQWRPEDVAKMP"
     gene            complement(1167..2498)
                     /locus_tag="VFF98_02880"
     CDS             complement(1167..2498)
                     /locus_tag="VFF98_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZU62602.1"
                     /translation="MSERITRRAIFAQAWPIILGQALVPLVSVVDVAVIGRLSDARNL
                     GGVALGTTILNLVLWTFGFLRMGVTGITAQARGRGEGAEICATLLRALALGLALGAGL
                     LVLAPAIVPASLALMAPPAGVGAAARGFLAMRLLGAPAALGFYAINGWLLGLGRTRLA
                     LGVQAVMNGVNIALDLLFVGLLGMGARGVGIGTTLAEWSALGCGLVAIGRALGPGWRA
                     QLGAVGRARLLDGRAIRRLVAVNVDIMVRTLALLSIFTWFTRSATALGPVPLAANHVL
                     MQMVSISAFVLDGFAFTAEARVGAAIGARSRASLLRAIRLTGEFSLGGGVLFAAGILL
                     VGRDAIAFLTPDPAIRALAGAMLPFCAAAPLVGMASWLLDGIFIGAVAGQALRNAALV
                     ALGLYLLADAALKPLGDAGIWLALLASYAFRAMALGLAVPALLRRVARDKT"
     gene            2726..4378
                     /locus_tag="VFF98_02885"
     CDS             2726..4378
                     /locus_tag="VFF98_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZU62603.1"
                     /translation="MAEDFTWDLEAEVVVLGSGGAAMTAAIAAHDFGAKDVVILERSG
                     MVGGTTAMSGGMLWIPGNHYQLEAGIAESDEDIVTYLDALAPGGLDPDTLGAFMEFGP
                     EMVRYLADKTPVRFHAYADFPDYQPYMPGAKPDGGRSLDNDAFSFERLGKWAARVNPT
                     KMAYPLRGSLMEAVSGSLDAATLAEREKGDYRGLGQALVGALFKAVLDREIPVQFEKR
                     ARKLVKDGDRVIGVVAEDAQGRDFHVRARRGVVIATGGFEWNEMLVKTFLRGPLTGPV
                     SVPENEGDGLLMAIEAGAQLGNMQNAFWQQSVLEFKPQHRAAKPNYMLGSDERARPGA
                     ILVNRAGKRFVNEAANYNAMGKALHAFDAGTHSYANLPYWLIIDQRYKDKYPTFNAQP
                     GGPLPSYMIQADTLEELAQKAGIEADGLVATVARFNDMVRKGHDDDFNRGDNTYDNFY
                     MWGDPAFEAPYRTLGLIDQGPYYAVKMESGALGTAGGPKTNGDAQVLDWHGNPIPGLY
                     AAGNAMAAVLGEAYGGAGGTLGPGLTFGYLAGRHLGSHISNH"
     gene            complement(4461..5963)
                     /locus_tag="VFF98_02890"
     CDS             complement(4461..5963)
                     /locus_tag="VFF98_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HZU62604.1"
                     /translation="MIDRRCAIKLAGAGAAAALVPGGAAMGAAESGQGGGTCLLSTTQ
                     GARWVAQPRPVLEADDGDPFSRSFAVALEDPRQTMRGFGAAFSEMGWAALGALPPAER
                     GKALDQLFGAGARLTHCRMPIGSSDMAHAPYSYAETPGDFALRDFSLGRDTQALVPFI
                     QAAQAREPQLVVWGSPWSPPRWMKTNNAFEMAPGWPGKPGNGLAPDQQRHEGEDAFIQ
                     QPRYFEAYARYFGRYVDAYAQHGIRIAAVMPQNEFNSAQPYVSCVWTPEGLARFLPWL
                     GREMGPRGVDVLLGTLERGNAELVARVLADPAAAAVVRGLGVQWAGRGALADIARRWP
                     QLPVWGSEQECGIGTNDWQYARYTWRALQDYLKHGACVWTYWNMALAEGGQSIAGWPQ
                     NALVAVDAARGRWRLTHDYWALRHTSAFVRPGARYLPTPSFIGYEFQTAFRNPDGSLV
                     VVMLNDMAEPQPRKIAIGHRQIRLSLPADSFTTLVLSAQEMAAAGLPAQG"
     gene            complement(6116..7030)
                     /locus_tag="VFF98_02895"
     CDS             complement(6116..7030)
                     /locus_tag="VFF98_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62605.1"
                     /translation="MRGYDMDLLRSLRVLVEEQSVVSAARRLQISEATMSRNLTRLRE
                     VFADPILVRSGRGMVASAMALALRDRVLALVNDADGLLEGPDIAAMGEMARSFTIRAN
                     DLMVASLADRLMAVFRREAPRCELIFAPETDDLVEDTLRHEQVDIYLGATSTLPPEIR
                     RQTLGRGRMRCVVRADHPILEEGISAEALVRFEHISVSRRGRARGPIDVALRERGLSR
                     RVTLVVPSYHAMVGTMRQSDMLLGLPDMVVERLPLEALGLAAFDYPFPLPVIIGYQAW
                     HPRWDNDPSHKWLRRNIFRMRRDIYCAQ"
     gene            7156..8838
                     /locus_tag="VFF98_02900"
     CDS             7156..8838
                     /locus_tag="VFF98_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62606.1"
                     /translation="MELHLHNPAPTPLAAPPLPAYIPPFGIRTVVGCLGVLLAVHVAG
                     FNEHITEIGLADIAGASHFGHDEGVWFTSTYEAFNIAAMAFTPWFYVTFSIYRFAIFM
                     TAALALFALPAPFMPDMASLCLLRALQGVASGCLPPILMTVMLKYLPPHLRVFGIGGY
                     AMSATFGPNLGGPLEAVLFGQLGWRWLYWETVPLCTLAVAMMAWGLPRDPSHLERFRQ
                     FNWRGFGLGLPGIVLLVTVLYHGERLDWFASPLIVHLTCAGGALFALFIVHEWFHHSP
                     FFRIQFWGNRNIAFALLTLVATLVVCGLLAELPMAFLAEVQGQRPGQIAPVALVVALP
                     QLILLPATAALCNTRRIDCRYVLAIGMGLLALSAFWGSRLSPEWMRGNFYAIQALQVF
                     GEPMVVIPVLMLATMKLGPADGPLISGMVNMMKGLANALTFALLATLLRRRENFHSAM
                     LLAGQGHSANALAGMGDPLAALTANLTPDARINAAAALETFHGYVHEQALTLALADLY
                     LLVCGVALVYMCLTALMPERVFPPSPPAAQPAPTLAPGPDAALPAPALLPAN"
     gene            8853..>9761
                     /locus_tag="VFF98_02905"
     CDS             8853..>9761
                     /locus_tag="VFF98_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU62607.1"
                     /translation="MSFSKPLVLAGASALVLIGGACLADRLLIGDSTTQATDDAYVEA
                     DFTTVAPKVAGRIDKVLVEDNEHVKPGQPLAHIEDADFRAALDMARGDVLAMQGEVAN
                     LEASIARQGAVVAEAAANVQADEASLTFARQNRARYSHLAEGGASPLELRQGAEARAA
                     ETEANRLRDSAAVDGARRQIAVLQAQLEKARGGLLRAEGAARQAELNLSYCTILAPVE
                     GLVGARGLRVGAYVHAGGALMAVVPNQNAYVVANFQETQLTRMKPGQQATIRIDTFPG
                     KLLHAHVDSLAPASEVAFSPIKPDNAT"
BASE COUNT         1615 a         3519 c         3152 g         1475 t
ORIGIN      
        1 ccggcatctt cgccacatcc tccggccgcc actgccattg cgagacagcg cgggcaaagg
       61 tggcggcctt ggcataggtg ccggcagtgg tgacggtctg cgcggcctgc acctgcccgt
      121 catcgccaat cacgaattcg acaaccgcca catcctccgg ctccagcccg gtatcggggc
      181 cgcaggcggg gggctccata aagacgggcc gcgcgaaggg ggtattggcc aaccgcccgg
      241 cgccagaggc agccatgtag cgataggcat ccccgttcct gttctgcaag cgcatggagg
      301 ctccggccag atcggcaaaa agcgccgaat cggcaatgcc gacaaccgcc ttctcgccgc
      361 cattcagggc ctgcgcctgc agcagctcgg cgcgggcctg cgccgcctgg ccctggttca
      421 gcaggacctt ggcgcgctgc cagtgccatt gggcgatttc ccgcctgtcc ggcacgggtt
      481 tcgcctcgga cagccgcaag ccctcctcga cagcggcaag cgcctgcgtg ccgccatcga
      541 acgctgtcac ctgggcgatt tccatcagtt cgggcagccg gtcgatgccc tgttccagcc
      601 tgagcgcggc ctgaaaatgc gcaagcgcgg tatcgtgatc ccagcgcgcc atggccagca
      661 cgcccagcat gttctcccca tccaccactt ccgcctggaa atcggcgcca gccgcgcgca
      721 gccgcggcag gccggccgcc agcaagggct cgccctctgg cgcatggccg gtgcgcacca
      781 ggcattcccc ccgccgcacc atgatcgcgg cctgcggcag cgaacccggc tggatccgcg
      841 catccgccgc cagccgcgca tagatcggca gggcaccagc gcaatcaccg cggccccagg
      901 catcgcttgc cgcatcgaac tgcgcctgca tactggtggc agcgcttccc ctgtcgggtg
      961 caggcgccac agtctgccca ctggcctgtg gctgaacagc aaccgatgcc gcagcgatgg
     1021 cgaacgcgaa tatggccttc acatccactc ccgttttctt atttttgaag ttatcgcaaa
     1081 tatacgcccc tatcgatatt gtcgatcagc tttgcagaaa gcgtattcgc tccagcaatt
     1141 gccgcgcaaa aatatcgaac cactacttag gttttgtccc tggccacccg ccgcagcagc
     1201 gccggcaccg ccagccccag cgccatggcc cggaaggcat agctggccag cagcgccagc
     1261 cagatcccgg catcgcccag cggcttcagc gcggcatcgg ccagcagata gaggcccagc
     1321 gccaccagcg ccgcattgcg cagggcctgg ccggccaccg cgccaatgaa aatgccgtcg
     1381 agcagccagc tggccatgcc caccagcggg gccgccgcgc aaaagggcag catggcgccg
     1441 gccagcgccc ggatcgccgg atcgggcgtg aggaaggcga tggcatcgcg gccgacaagg
     1501 aggatgccgg cggcaaacag cactccgccg cccagcgaaa actcccccgt caggcggatg
     1561 gcgcgcagca ggctggcgcg cgagcgggcg ccgatcgccg cgcccacccg cgcctcggcg
     1621 gtgaaggcaa aaccatcgag cacgaaggcg ctgatgctga ccatctgcat caacacatga
     1681 ttggccgcca gcggcaccgg ccccagcgcc gtggccgagc gggtgaacca ggtgaaaatc
     1741 gacagcaggg ccagcgtgcg caccatgata tccacgttga cggcgaccag ccgccggatc
     1801 gcccgcccgt cgagcaggcg tgcccggccc accgcgccca gctgggcccg ccagcccggc
     1861 cccagcgccc ggccgatcgc cacaaggccg cagccaagcg cgctccattc cgccagcgtg
     1921 gtgccgatgc caacgccgcg cgcgcccatg cccagcaggc cgacgaacag cagatcgagg
     1981 gcaatgttca ccccgttcat caccgcctgc acgcccagcg ccagccgcgt gcgccccagc
     2041 cccagcagcc agccgttgat cgcatagaag ccaagcgctg ccggcgcgcc cagcaggcgc
     2101 atggccagaa acccgcgcgc cgccgcgccc acgcccgcag gcggggccat cagcgccagg
     2161 ctggccggca cgatcgccgg cgccagcacc agcagccccg cgccaagggc cagccccagc
     2221 gccagcgcgc gcagcaatgt ggcgcagatc tcggccccct cgccgcgccc gcgcgcctgc
     2281 gcggtgatgc cggtgacgcc catcctgagg aagccgaagg tccagagcac gaggttgagg
     2341 atggtggtgc ccagcgccac cccgcccaga ttgcgcgcat cgctcagccg gccgatcacc
     2401 gccacatcca ccacgctgac cagcggcacc agcgcctggc ccagaatgat cggccaggcc
     2461 tgcgcgaaaa tggcgcgccg ggtgatcctc tcgctcacgg tgtccctcga tgcatgcgcc
     2521 agccccttgt ttcaggcggc cttctggcac ggcagggctg cggctggcta ccggcaaaac
     2581 ccgcccgccg cgtgttaccc tagaacattg tggagtggcc cgcgcgcgga gggaacctag
     2641 agtggcccgg gcaaacgaga ggcggataaa agcgcaggca gccgacagca gttccggcac
     2701 cagcgcctat cccgggagat ggatgatggc agaagacttc acatgggatc tggaggccga
     2761 ggtcgtcgtt ctcggctctg gtggcgcggc gatgacagcg gcgatcgccg cccatgattt
     2821 cggcgcgaaa gacgtggtga tcctcgaaag gtcggggatg gtgggcggca ccaccgccat
     2881 gtcgggcggc atgctgtgga tcccgggcaa tcactatcag ctcgaggccg gcatcgccga
     2941 atccgacgag gacatcgtca cctatctcga tgccctcgcc cccggcgggc tcgatcccga
     3001 cacgctgggc gccttcatgg aattcggccc cgagatggtg cgctatctgg cggacaaaac
     3061 gccggtgcgc ttccacgcct atgccgattt ccccgattac cagccctata tgcccggcgc
     3121 caagcccgat ggcggccgct cgctcgacaa tgacgccttc tccttcgagc ggctgggcaa
     3181 atgggccgcg cgcgtcaacc ccaccaagat ggcctatcca ctgcgcggca gcctgatgga
     3241 agcggtcagc ggctcgctcg acgcagccac gctggccgag cgcgagaagg gcgattatcg
     3301 cgggctgggc caggcgctgg tcggcgcgct gttcaaggcg gtgctcgacc gcgagatccc
     3361 cgtgcagttc gagaagcgcg cgcgcaagct cgtcaaggat ggcgaccggg tgatcggcgt
     3421 ggtggccgag gatgcccagg gccgcgattt tcacgtgcgc gcccggcgcg gcgtggtgat
     3481 cgccaccggc ggcttcgagt ggaacgagat gctggtgaag accttcctgc gcggccccct
     3541 caccggccct gtcagcgtgc ccgagaacga aggcgatggc ctgctgatgg cgattgaagc
     3601 cggcgcccag ctgggcaaca tgcagaacgc cttctggcag cagagcgtgc tggaattcaa
     3661 gccccagcac cgcgccgcca agcccaacta catgctgggt tccgacgagc gcgcccggcc
     3721 cggcgccatc ctcgtcaacc gcgcgggcaa gcgctttgtc aacgaggcgg ccaactacaa
     3781 cgccatgggc aaggcgctgc acgccttcga tgccggcacc cacagctatg ccaacctgcc
     3841 ctactggctg atcatcgacc agcgctacaa ggacaagtat cccaccttca acgcccagcc
     3901 cggcggcccc ctcccctcct acatgatcca ggccgacacg ctggaggaac tggcccagaa
     3961 agccgggatc gaggccgatg ggctggtggc caccgtggcg cgcttcaacg acatggtgcg
     4021 caagggccat gacgacgact tcaaccgcgg cgacaacacc tacgacaatt tctacatgtg
     4081 gggcgaccct gccttcgagg cgccctatcg cacgctgggg ctgatcgatc agggccctta
     4141 ttatgcggtg aagatggaat cgggcgcgct gggcaccgcc ggcggcccca agaccaatgg
     4201 cgatgcccag gtgctcgact ggcatggcaa ccccatcccc gggctgtatg ccgccggcaa
     4261 tgccatggcg gccgtgctgg gcgaggccta tggcggggcc ggcggcacgc tggggccggg
     4321 cctcaccttc ggctatctgg ccgggcgcca cctgggcagc catatctcga accactgatg
     4381 ggctgatgca tgccggccgg cgcccccgca ccagccggca tgccccccac cacccctgcg
     4441 cgcgcgcctt acgcgcgccc ttacccttgg gcaggcaggc cggccgcggc catctcctgc
     4501 gccgagagca ccagcgtggt gaaactgtct gccggcaggc tgaggcggat ctgccggtgg
     4561 ccgatggcga tcttgcgcgg ctgcggctcg gccatatcgt tcagcatcac caccaccagg
     4621 ctgccatcgg ggttgcggaa ggcggtctgg aactcatagc cgatgaagct gggcgtgggc
     4681 agataacggg cgccgggccg cacgaaggcg ctggtgtggc gcagcgccca atagtcatgc
     4741 gtcaaccgcc agcggccgcg cgccgcatcc accgccacca gcgcattctg cggccagccg
     4801 gcaatgcttt gcccgccttc ggccagcgcc atgttccaat aggtccacac gcaggcgccg
     4861 tgcttcaggt aatcctgcag ggcgcgccag gtgtagcggg catattgcca gtcgttggtg
     4921 ccaatgccgc attcctgctc gctgccccac accggcagct gcggccagcg ccgggcgata
     4981 tcggccagcg cgccgcgccc ggcccattgc acgcccagcc cgcgcaccac ggccgccgcg
     5041 gcaggatcgg ccagcaccct ggcgaccagc tcggcattgc cgcgttccag cgtgcccagc
     5101 agcacatcca ccccgcgcgg gcccatctcg cggcccagcc agggcaggaa gcgggccagc
     5161 ccttccggcg tccacacgca ggagacatag ggctgggccg agttgaactc gttctgcggc
     5221 atcaccgcgg caatgcggat gccatgctgc gcataggcat cgacatagcg gccgaaatag
     5281 cgggcatagg cctcgaaata gcgcggttgc tggatgaagg catcctcgcc ttcgtggcgc
     5341 tgctgatcgg gcgcgaggcc attgcccggc ttgcccggcc agcccggcgc catctcgaag
     5401 gcgttgttcg tcttcatcca gcgcggcggg ctccacggag agccccagac gacaagctgc
     5461 ggctcacgcg cttgcgcagc ctggatgaag ggcaccagcg cctgtgtatc acggccaagg
     5521 gagaagtcgc gcagggcaaa atcgcccggc gtctcggcat agctgtaagg cgcatgggcc
     5581 atgtcgctac tgccgatcgg catgcggcaa tgggtgagcc gggcgccggc gccaaacagt
     5641 tggtcgagcg ccttgccgcg ctcggccggt ggcagggcgc ccagcgcggc ccagcccatc
     5701 tcgctgaaag cggcgccaaa gccgcgcatc gtctggcgcg gatcttccag cgccaccgcg
     5761 aaggaacggc tgaacggatc gccatcatcc gcttccagca ccgggcgcgg ctgcgccacc
     5821 cagcgcgcgc cctgcgtggt ggagagcagg caggtgccac cgccctgccc gctttcggca
     5881 gcgcccatgg ccgcgccccc cggcaccagc gcggcagcag cgccggcccc ggcaagtttg
     5941 atggcacagc ggcggtcgat catggcgggc aggctttctg caaggcggca acatccctgg
     6001 cccgccgccg gcatgcagct gcggcaaacc cttgaattgc acagaatgag aatcgtttac
     6061 atgatgatgc cgcaatcgcc ggtgattgca agcggatgat cgccggccac cccgctcatt
     6121 gcgcgcaata gatatcccgg cgcatgcgga agatgttgcg gcgcagccat ttgtgcgagg
     6181 gatcgttatc ccatcgcggg tgccaggcct gataaccgat gatcaccggc agcgggaagg
     6241 gatagtcgaa agcggccagc cccagcgcct cgagcggcag gcgctccacc accatgtcgg
     6301 gcaggcccag cagcatgtcg gactggcgca tggtgcccac catcgcatga tagctgggca
     6361 ccaccagcgt tacccgccgg ctgagccccc tttcgcgcag cgccacatcg atgggcccgc
     6421 gcgcccgccc ccggcgcgaa acgctgatat gctcgaaacg caccagcgcc tcggccgaga
     6481 tgccttcctc gaggatggga tgatcggccc gcaccacgca gcgcatgcgc ccgcgcccca
     6541 gcgtctgccg gcggatctcg ggcggcaggg tgctggtggc gcccagatag atatccacct
     6601 gctcgtggcg cagcgtgtct tccaccagat catcggtttc cggcgcgaaa atcagctcgc
     6661 agcgtggcgc ctcgcggcgg aacaccgcca tcagccgatc cgccagcgag gccaccatca
     6721 gatcgttggc gcgaatggtg aagctgcgcg ccatttcgcc catggcggcg atatcgggcc
     6781 cctccagcag gccgtcggca tcgttgacca gcgccagcac ccgatcacgc agcgccagcg
     6841 ccatggccga ggccaccatc ccccgccccg agcgcaccag aatgggatcg gcgaagacct
     6901 cgcgcaggcg ggtgaggttg cggctcatcg tcgcctcgct gatctgcagg cgccgcgccg
     6961 ccgacaccac gctctgctcc tcgacgagca ccctgagcga gcgcagcagg tccatgtcat
     7021 atccgcgcat acagtcctcc cggcatgcct cttccatagc gggcaaatcg cgcccgagga
     7081 ccatcaatta cgcaagcctg ccacagcaga actgacggtt ttttccgcat tgcagcaatg
     7141 gaagaagccg cgcccatgga actgcacctg cacaatccgg cgccaacgcc gctggccgcg
     7201 ccgccgctgc ccgcctatat cccgcccttc ggcatccgca cggtggtggg gtgcctgggg
     7261 gtgctgctgg ccgtgcatgt cgccggcttc aacgagcata tcaccgagat cggccttgcc
     7321 gacatcgccg gcgccagcca tttcggccat gacgagggcg tgtggttcac ttccacctac
     7381 gaggccttca acatcgccgc catggccttc acgccgtggt tctatgtcac cttctcgatc
     7441 tatcgcttcg ccattttcat gaccgcggcg ctggcgctgt ttgccctgcc ggcgccgttc
     7501 atgcccgata tggcctcgct gtgcctgctg cgggcgctgc agggggtggc ctcgggctgc
     7561 ctgccgccga tcctgatgac ggtgatgctg aaatatctgc cgccgcatct gcgcgtgttc
     7621 ggcattggcg gctatgccat gagcgccacc ttcggcccca atctgggcgg cccgctggaa
     7681 gcggtgctgt ttggccagct gggctggcgg tggctctatt gggaaacggt gccgctgtgc
     7741 acgctggcgg tggcgatgat ggcctggggc ctcccgcgcg atccgtccca tctcgaacgt
     7801 ttccggcagt tcaactggcg cggctttggc ctgggcctgc cggggatcgt gctgctggtc
     7861 accgtgctct atcatggcga gcggctggac tggttcgcct cgccgctgat cgtccacctc
     7921 acctgcgcag ggggcgcgct gtttgccctg ttcatcgtgc atgaatggtt tcaccacagc
     7981 cccttcttcc gcatccagtt ctggggcaac cgcaacatcg cctttgccct gctgacgctg
     8041 gtggcaacgc tggtggtgtg cgggctgctg gccgagctgc cgatggcctt cctggccgag
     8101 gtgcagggcc agcggcccgg gcagatcgcc cctgtggcgc tggtggtggc gctgccgcag
     8161 ctgatcctgc tgccggcaac ggcggccctg tgcaacaccc gccgcatcga ttgccgctat
     8221 gtgctggcga tcggcatggg gctgttggcg ctgtcagcct tctggggctc gcgcctgtcg
     8281 cccgaatgga tgcgcggcaa tttctacgcg atccaggcgc tgcaggtgtt tggcgagccg
     8341 atggtggtga tcccggtgct gatgctggca acgatgaagc tgggcccggc cgatgggccg
     8401 ctgatttcgg gcatggtcaa catgatgaag gggctggcca atgcgctcac cttcgcgctg
     8461 ctggccacgc tgctgcgccg gcgcgagaac ttccattcgg ccatgctgct ggccggccag
     8521 ggccacagcg ccaatgcgct ggccggtatg ggcgatccgc tggccgccct caccgccaac
     8581 ctcacgcccg atgcccgcat caacgccgcc gccgcgctgg agaccttcca tggctatgtg
     8641 cacgagcagg ccctcaccct ggcgctggcg gatctctatc tcctcgtctg cggtgtggcg
     8701 ctggtttaca tgtgccttac cgcgctgatg cccgagcggg tgttcccgcc cagcccgccg
     8761 gccgcccagc ccgcgcccac tctcgcaccc gggccagatg ccgccctgcc ggctcccgcc
     8821 cttctgcccg ccaactgacg gaacctcgaa ccatgtcctt ttccaagcca ctggtgctgg
     8881 ccggtgccag cgccctcgtg ctgatcggcg gcgcctgcct tgccgaccgc ctgctgatcg
     8941 gcgacagcac cacccaggcc accgacgatg cctatgtcga ggccgatttc accaccgttg
     9001 cccccaaggt ggccggccgc atcgacaagg tgctggtgga agacaacgag cacgtgaagc
     9061 ccggccagcc gctggcccat atcgaggatg ccgattttcg cgccgcgctc gacatggccc
     9121 gcggcgatgt gctggcgatg cagggcgaag tggccaatct tgaggccagc atcgcccggc
     9181 aaggcgcggt ggtggcagaa gcggccgcca atgtgcaggc cgatgaggcc agcctcacct
     9241 ttgcccgaca gaaccgcgcg cgctacagcc atctggccga aggcggcgcc agcccgctgg
     9301 aactgcgcca gggcgccgag gcccgcgccg ccgaaaccga ggccaaccgc ctgcgcgaca
     9361 gcgccgcggt ggatggcgcc cggcgccaga tcgccgtgct gcaggcccag ctggagaagg
     9421 ccaggggcgg cctgctgcgc gctgagggcg ctgccagaca ggcagagctg aacctctcct
     9481 attgcaccat cctcgcgccg gttgaagggc tggtgggcgc gcgcgggctc agggtgggcg
     9541 cctatgtcca tgccggcggc gcgctgatgg cggtggtgcc caaccagaac gcctatgtcg
     9601 tggccaattt ccaggaaacc cagctgaccc gcatgaagcc cggccagcag gccacgatcc
     9661 gcatcgacac tttccccggc aagctgctgc acgcccatgt ggacagcctg gcgccggcca
     9721 gcgaagtggc cttctcgccc atcaagcccg acaatgcgac c
//
LOCUS       DASRYE010000050         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_160720,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000050 DASRYE010000000
VERSION     DASRYE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160720"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1090)
                     /gene="trpS"
                     /locus_tag="VFF98_02910"
     CDS             complement(74..1090)
                     /gene="trpS"
                     /locus_tag="VFF98_02910"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZU62608.1"
                     /translation="MRIVSGIQPTGNLHLGNYLGAIRNWVAMQDEAAATGAELFFFLA
                     DLHAISVGHVPAELAANTREMVAALVACGVDPAKSVLFNQAQVPAHAELQWLLNGTAR
                     IGWLNRMTQFKDKAGKNRDSASVALYTYPVLMAADVLVYNATHVPVGEDQKQHLELAR
                     DVAQKFNNDFCPEEAPLFTLPAPIIPPTAARIMSLRDGTAKMSKSDPSDMSRINLTDD
                     AEAIMNKVKKAKTDPEPLPSEKEGLEGRAEARNLVGIYAAMADVSVEQVLADFGGKGF
                     GAFKPALGELLVEKLSPINARFNALRADRAALDAILHEGAGKARAAAAPVLSAAYAAL
                     GLVR"
     gene            complement(1123..2688)
                     /gene="murJ"
                     /locus_tag="VFF98_02915"
     CDS             complement(1123..2688)
                     /gene="murJ"
                     /locus_tag="VFF98_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZU62609.1"
                     /translation="MNLLKATGTIGGLTLVSRILGLVRDSLFARVVGAGFASDAFLVA
                     FRLPNMFRALFAEGAFASAFIPMFNQKVADPEGQGLASGLDFAEQALSVLLPTLLAMT
                     VLLEAFAWPVTWALSGKFHGVTPAQFGYAVLLSRLTIPYLAFISLVSLLGGILNSLHK
                     FWVNAAAPILLNLTLIVAILLFRSHDPLITARNQALAVSVSGALQLAWLAHACWRNGV
                     SLRPRLPRLTPEVKRLMALIWPAAAGAGAVQINLVISTALAASLLDHGSVTYIYMADR
                     LNQLPLGLIGIGLGTVLLPTISRQLGAGEEAAAMATQNRGLELALALTLPATMGLVFC
                     GEPIAAALFGYGRYSAADTHHTAQALAAFSVGLPSYILVKVLTPGFYARQDTKTPVRY
                     AMIAMAVNLVLNLVLVLPLAHMGPPLATAISSTVNVAMLYATLVKRGHFRPDAQLRQR
                     AWRLALAALTMGAVMAISEGRLMPYVHGGWLMRIGALAVLVGAGVCVYAAATVALGAL
                     GREDLALLLRRRR"
BASE COUNT          508 a          923 c          905 g          386 t
ORIGIN      
        1 agggtacggc cgggtggggg agagggccgg cgctgcaagg aaacgcgccc cgaagggcgc
       61 gcgcctcaca ccatcagcgc accagcccca gcgccgcata ggcggccgac agcaccggcg
      121 ctgccgccgc ccgcgccttg cccgcgccct cgtgcaaaat ggcatcgagc gcggcgcgat
      181 cggcgcgcag ggcattgaag cgggcgttga tcgggctcag cttctccacc agcaattcgc
      241 ccagcgccgg cttgaaggcg ccaaagccct tgccgccaaa atcggccagc acctgctcca
      301 cactcacatc ggccatggcg gcatagatgc ccaccagatt gcgcgcctcg gcccggcctt
      361 ccagcccttc cttctccgag ggcagcggct cggggtcggt cttggccttc ttcaccttgt
      421 tcatgatcgc ctcggcatca tcggtcaggt tgatgcggct catgtcgctg gggtcggatt
      481 tgctcatctt ggcggtgcca tcgcgcagcg acatgatgcg cgcggccgtg ggcgggatga
      541 tcggcgccgg cagggtgaac agcggtgctt cctcggggca gaaatcattg ttgaacttct
      601 gcgccacatc gcgcgccagt tcgagatgct gcttctggtc ctcgcccacc ggcacgtggg
      661 tggcattgta gaccagcaca tcggcggcca tcagcaccgg ataggtgtaa agcgccaccg
      721 aggccgaatc gcggttcttg ccggccttgt ccttgaactg ggtcatgcgg ttcagccagc
      781 cgatgcgggc ggtgccgttc agcagccact gcagctcggc atgggcgggc acctgggcct
      841 ggttgaacag caccgatttg gccggatcca ccccgcaggc caccagcgct gccaccatct
      901 cgcgcgtgtt ggcggccagt tccgccggca catggcccac cgagatggcg tgcagatcag
      961 ccaggaagaa gaacagctcg gcgccggtgg cggcagcctc gtcctgcatc gccacccagt
     1021 tgcggatcgc gcccagataa ttgccaaggt gcagattgcc ggtgggctgg atgccggaaa
     1081 cgatacgcat agaaaagggc tcgcctcgtt ggagaaaaga gttcagcggc ggcggcgcag
     1141 caacagggcc agatcttccc gacccagcgc ccccagcgcg accgttgccg ccgcatagac
     1201 gcaaacgccc gcccccacca gtactgccag cgcgccgatc cgcatcagcc agccgccatg
     1261 cacatagggc atcagccggc cctcgctgat cgccatcacc gcgcccatcg tcagcgcggc
     1321 cagcgccagg cgccaggcgc gctggcgcag ctgcgcatcg ggacggaaat ggccgcgctt
     1381 caccagcgtg gcatacagca tcgccacatt caccgtggag gaaatggcag tcgccagcgg
     1441 cggccccata tgcgccagcg gcagcaccag cacgaggttc agcaccaggt tcaccgccat
     1501 ggcgatcatc gcatagcgca cgggcgtctt ggtgtcctgc cgggcataga agccgggggt
     1561 gagcaccttg acgaggatat agctgggcag gcccaccgag aaggcggcca gcgcctgggc
     1621 ggtgtgatgc gtgtcggccg cgctgtagcg gccatagccg aagagcgccg cggcaatcgg
     1681 ctcgccgcag aagaccaggc ccatggtggc cggcagcgtg agcgccagcg ccagttccag
     1741 cccgcggttc tgcgtggcca tggccgccgc ttcctcgccc gcgcccagct ggcgtgagat
     1801 ggtgggcagc agcaccgtgc caaggccaat gccaataagg cccagcggca gctggttcag
     1861 ccggtcggcc atgtagatat aggtgacaga gccgtgatcg agcaggctgg cggccagcgc
     1921 ggtggaaatc accaggttga tctgcaccgc gcccgcgcct gccgctgccg gccagatcag
     1981 cgccatcagc cgcttcacct cgggcgtgag gcgcggcagg cgcgggcgca gcgaaacgcc
     2041 attgcgccag caggcatggg ccagccaggc cagctgcaac gcccctgaca ccgacaccgc
     2101 cagcgcctgg ttgcgcgcgg tgatcagcgg atcatggctg cgaaacagca ggatggcgac
     2161 aatcagcgtg aggttgagga gaatgggcgc ggccgcattc acccagaact tgtgcagcga
     2221 attgaggatc ccgcccagca gcgacaccag gctgatgaag gccagatagg ggatcgtcag
     2281 ccgcgagagc agcaccgcat agccgaactg cgcaggcgtg acgccatgga acttgcccga
     2341 cagcgcccag gtcaccggcc aggcgaaggc ttccagcagc accgtcatcg ccagcagcgt
     2401 aggcagcagc acggaaagcg cctgttcggc aaaatcgagg cccgaggcca gcccctgccc
     2461 ttccggatcg gccaccttct gattgaacat cgggatgaag gccgaggcaa aggccccttc
     2521 ggcaaacagc gcccggaaca tgttgggcag gcgaaaggcc accagaaagg cgtcggaggc
     2581 aaagcctgcc cccaccacgc gcgcgaacag cgaatcgcgc acaaggccca gaatgcggct
     2641 gacgagcgtg agcccgccga tcgtgccggt ggctttgaga aggttcatgg caactgcttt
     2701 gctgggcacg cgcccgcggc gg
//
LOCUS       DASRYE010000051         8905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_19120,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000051 DASRYE010000000
VERSION     DASRYE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19120"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1489
                     /gene="hemN"
                     /locus_tag="VFF98_02920"
     CDS             173..1489
                     /gene="hemN"
                     /locus_tag="VFF98_02920"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HZU62610.1"
                     /translation="MWPYYPDLLARPVPRYTSFPTAAEFSDAVGGDALDGALAATSGD
                     VSLYVHIPFCEQICWYCGCNTGAANKRARLTSYLDALGREVALVGKKLDGRVRVKRVA
                     FGGGSPNAISPTDFVRLVDAITLHFRLDDPVWSIELDPRTMTHEWGQVLSFVGVTRAS
                     LGVQTFSPTLQQAIGRVQPDSVIEACTGLLRDSGITSLNYDLMYGLPGQTMDDLDQTL
                     ARACALGADRIALFGYAHVPHMLPRQRRIDASALPGQEARFDMAAHGYGVLVGEGYAP
                     VGFDHFARPGDTLAQATTSGRLRRNFQGFTEDQAPVLIGLGASAISAFPDLLVQNEKN
                     AGRYRMLLSQDRLPGAHGVRRSAEDRMLGEIVMGLLCQGSAVVAGERAWEVAAAGLAP
                     FIAHGLVEVNHGRVTIMPGGLPYARSIAACFDPYRQQSARRFSSAV"
     gene            complement(1833..3956)
                     /locus_tag="VFF98_02925"
     CDS             complement(1833..3956)
                     /locus_tag="VFF98_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZU62611.1"
                     /translation="MTDFSSPFRYLRPVALLWQWLALAAAMALSPPARADVPPAQPAI
                     TPAIAPHITATLLAERPARPGETILLAIAMAPAAGWHGYWQNPGDAGFAMRLEWALPA
                     GARVAAPLYTVPETLLVGGLMNHVYQRDYAVLVPFTLPAGAAGSLPVALKAQWLACSA
                     QICVPEEATLHTSVTVGTGAADPRFDGWRAHLPAPLDAPAQLAASGSHLRLAIPFPAG
                     AAIEAPHFFPAADGVVDYAAPQGFARKGDVLILTLARAHNAALPASLPGVLRFNRAGD
                     GLAITTRPGPVPTGGTPLADGTGSTGGFGWLLLAAVVGGLLLNVMPCVFPILSLKALS
                     LARAGESAAQARAEGLAYAAGVVLACVALGAAMLALRAAGEEVGWAFQLQEPGVVAAL
                     LLLAAAITANLAGLFELAVPGFIASGAGATRAGGINGAFATGLLGAFVATPCTGPFMA
                     GAMGAALVLPVGPALALFAGLGLGIALPFLAIAFVPLLRRALPRPGGWMGWLRRLLAL
                     PMGLTALALLWLAGRLGGSVFMAGLGLSAAGLLLVLALIGRWQRRGRAAAGRGLALLA
                     ALGAAAGLLLPGTIAKPAATEADLLAEQPFTDAALAAARAQGRPVFAYFTADWCLTCK
                     VNERLAIETAATRDAFAKAGVVVLKGDWTRRDPAITRYLASQGAAGVPLYVWYPARGG
                     PALVLPQVLASDTLGQLAASSSRPQ"
     gene            4064..5911
                     /locus_tag="VFF98_02930"
     CDS             4064..5911
                     /locus_tag="VFF98_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZU62612.1"
                     /translation="MTQAPARPLSRSFAHRRWLAGLMAGVGLLAGGALQAKPVAHHAA
                     HPVAAAAPAPATPPKLLVAIAVDQFSADLFAQYRQHYSAGMARLLTGAVFPSGFQSHA
                     ATETCPGHSTLLTGVHPARSGIIANNWYDTAAARPDKRIYCAEDDHHPDSDSKNPIVS
                     AVHLKVPTLGEMMKARWPESRNVAVSVKDRAVMMMGGHQTDAAYWLVARGFETYHNQP
                     IGNEALDENASILALLNKGAPAFTPPAWCAPRDRAITTRNGAGVVPSPAALADGPTVV
                     GKGRFALDAGDADGFKVSPRADAATLDLALRLVRAMKLGKGAAPDLLSVSLSATDYIG
                     HAYGTEGMEMCIQQNQLDLALGAFFAQLDALGLDYAVVLTADHGGLDIPERLEDQADP
                     AARRAAAGLSSFALNEDIARELGLTAFLPRREADGTLAIAQPAYPKQLVYAESAFGDY
                     FITHELSEEQRQKVQALLVAKLKASPLVAHVWTKAEIASFPIAHGNPQDWTMEERVRA
                     SWDAQRSGDVFSVLKRTVVPIPEPRPGYTATHGSPWDYDRRVPMLFWRRGYTGFEQPQ
                     PVETVDIAPSLAALVGLAEPDGTFDGRCLDLDGGAKNTCEGAAAVPSRH"
     gene            5911..7143
                     /locus_tag="VFF98_02935"
     CDS             5911..7143
                     /locus_tag="VFF98_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU62613.1"
                     /translation="MDNTRDLVILGGGLVGMTLALAAARQGITSHVVEREDPADLTAE
                     GVDGRASAISSASWNLFRNIGLAEALTPLGCPIDAIAVTDQHKPGRIDFRPDPGAESL
                     GRMYANRDIRLALFEAARHEPAIAWHTHAQVTGRERGPHGVAATLADGQVLKGSLLVG
                     AEGRRSPTREEEGIGLVAWDYRHRAIVTALAHEKPHDNVAWEIFYAAGPFALLPLNDL
                     PEPTARGYRHRSALVWTVPEKDAAGVTALSDATFLAEVARRMEGLFGGIELIARRMAY
                     PLTYQNATRITGERLALVGDAAHGMHPIAGQGLNLGLRDVGALVEVLADGMRLGLEPG
                     DAQLLKRYENWRALDIFSTMSVMDGIVRLFGVPGRKASAIRRLGMAGVQRMPLLKRFF
                     MDEARGLSGHLPALLRAA"
     gene            complement(7163..8137)
                     /locus_tag="VFF98_02940"
     CDS             complement(7163..8137)
                     /locus_tag="VFF98_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62614.1"
                     /translation="MAEDLPNQTRKVLRRRRWRALMVMAGLGFVLGSGGVVALGWGLG
                     LVPGRLAVAPAPLAAPAPLPAASAPGPQGAVEWRVSALEQRLARLDLESAAAEGNAAR
                     AEALLVAFAARRVVERGATLGYLEDQLKLRFGGAQPEAVATIIATARQPVTLDRLAAQ
                     LDAMAPRLANPPSDESVWTRLQRELANLFVLRREAGTPAPDPAFRLDHARMCLREGRV
                     EDAIADVERMPGSAAAAQGWIAGARRYIAVERALDLIETTAVLEPRTLRDNSGNRVVQ
                     PSPLALPPPDAGAEPDEAPATPPAPAAAASSAPLPARTPAPAATGPAK"
     gene            complement(8162..8884)
                     /locus_tag="VFF98_02945"
     CDS             complement(8162..8884)
                     /locus_tag="VFF98_02945"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZU62615.1"
                     /translation="MSLPLILLRPEPGLAASAAAAHAMAPGVEVVAAPLFAVAGVAWA
                     APAPAAVDALLLGSANALRHAGPQLAAFAGKPAYAVGAATAEAARAAGLDVVAVGEGG
                     LQALLAHLAPAHRRLLRLAGEERVELTPPPGVALAEQVVYRLAALPLAGPVALLLQAR
                     ALPGAVVALHSAAAARHFAAECARLGVDRRRIVLATIGRRVATAAGEGWRDILVAHRA
                     EDAALLALALQTCQNPALIQRG"
BASE COUNT         1308 a         3151 c         3177 g         1269 t
ORIGIN      
        1 ggacggggcc gccgcgcgca tcctgatcga cgaggagggc gagggcgccg gccgccctga
       61 aggcgcgggc gcgcccggcc cgcaaggcga agcggggcag atcaggcgct gatcttgccg
      121 tgcttgatcg cgcgcaaaga aaagccccgg ccgctttggc acaccaatgc ccatgtggcc
      181 ttactatccc gacctgctgg cccggcccgt gccgcgctac accagctttc ccaccgccgc
      241 ggagttttcc gatgcggtgg gcggcgatgc gctggatggg gcgctggcgg ccaccagcgg
      301 cgatgtgtcg ctctatgtcc acatcccctt ttgcgagcag atctgctggt attgcggctg
      361 caacaccggc gctgccaaca agcgcgcgcg gctgacctcc tatctcgatg cgctgggccg
      421 cgaagtggcg ctggtgggca agaagctgga tggccgcgtg cgggtgaagc gggtggcctt
      481 tggcgggggc agccccaatg ccatttcgcc caccgatttc gtgcggctgg tggatgccat
      541 cacgctgcat ttccggctgg atgatccggt gtggtcgatc gagctggacc cgcgcacgat
      601 gacgcatgaa tgggggcagg tgctctcctt cgtgggcgtg acgcgggcca gccttggcgt
      661 gcagaccttc tcgcccacgc tgcagcaggc gattggccgc gtgcagcccg acagcgtgat
      721 cgaggcctgc accggcctcc tgcgcgattc cggcatcact tcgctcaact atgatctcat
      781 gtatggcctg ccgggccaga cgatggacga tcttgaccag acgctggcgc gggcctgcgc
      841 gctgggggcc gatcgcatcg cgctgtttgg ctatgcccat gtgccgcaca tgctgccgcg
      901 ccagcgccgc atcgatgcca gcgccctgcc cggccaggaa gcgcgcttcg acatggccgc
      961 gcatggctat ggcgtgctgg tgggcgaggg ctatgcgccg gtgggcttcg accatttcgc
     1021 ccgccccggt gatacgctgg cccaggccac taccagcggc cggctgcggc gcaatttcca
     1081 gggctttacc gaggatcagg cgccggtgtt gatcgggctg ggggcatcgg ccatctcggc
     1141 cttccccgat ctgctggtgc agaacgagaa gaacgccggg cgctatcgca tgctgctctc
     1201 gcaggatcgg ctgccgggcg cccatggcgt gcgccgcagc gccgaggacc ggatgctggg
     1261 cgagatcgtc atgggcctgc tgtgccaggg cagcgcggtt gttgcgggcg agcgggcctg
     1321 ggaagtggcg gcggccggcc ttgcgccctt catcgcccat gggctggtgg aagtgaacca
     1381 cggccgggtg acgatcatgc ccggcggcct gccctatgcg cgcagcattg ccgcctgttt
     1441 cgatccctat cgccagcaat cggcgcggcg tttttcctcg gcggtctagg ccgggttggg
     1501 ccgggcgcgg ggcagcggtt cccggtgcgg cattgcggcc acaccggcac gggaaatgcc
     1561 cgtttgcgtc cccctccttg ctttgccgtt gaccatgcgc tgatttgtcg gcaaacacac
     1621 aaaaaaacag gcggctgaca caatggacag ccgcctaggt ggggagaaag ctctcaaaat
     1681 cgggatggcg acaatggcct gttggcctga tcgccctggc ggtgtggagt gaaccatgcc
     1741 gccgtcttga gagcttatgt atgctgcact gcgggggcac gccttgatcg gcgtcaaacc
     1801 tccgcttgat tgtagtcaaa agcgcgtttt tcttattgcg gccggctgga tgacgccgcc
     1861 agctggccca gcgtgtcgct ggccagcact tgcggcagca ccagcgccgg cccgccccgc
     1921 gccggatacc acacataaag cggcacgccc gccgcgccct ggctggcaag atagcgggtg
     1981 atcgccggat cgcgccgggt ccagtcgccc ttcagcacca ccacgccggc tttggcgaag
     2041 gcatcgcggg tggcggcggt ctcgatggcg aggcgctcgt tcaccttgca ggtaaggcac
     2101 cagtccgccg tgaaataggc gaagacgggg cgcccctgcg cccgcgcggc ggccagcgcg
     2161 gcatcggtaa agggctgctc ggccagaagg tcggcctctg tggccgcggg tttggcgatg
     2221 gtgccgggca gcagcaggcc ggcggccgcg cccagcgctg ccagcagcgc caggccccgg
     2281 ccggccgccg ccctgccgcg ccgctgccag cgcccgatca gcgccagcac cagcagcagc
     2341 cccgctgccg acagccccag cccggccatg aacacagagc cccccagccg gccagccagc
     2401 cacagcaggg ccagcgccgt caaccccatg ggcagggcca gcaggcggcg cagccagccc
     2461 atccagccgc ccgggcgcgg cagggcgcgg cgcagcagcg gcacgaaggc aatggccaga
     2521 aagggcaggg caatgcccag ccccagcccg gcaaacagcg ccagcgccgg ccccaccggc
     2581 agcaccagcg ccgcgcccat ggcgccggcc atgaacggcc cggtgcaggg cgtggcgaca
     2641 aaggcgccca gcagccctgt ggcaaaggcg ccgttgatgc cgccggcccg cgtggccccg
     2701 gcgccgctgg caatgaagcc gggcacggcc agttcgaaca ggccagccag attggcggtg
     2761 atggccgcgg ccagcagcag cagcgcggcc accacgccgg gttcctgcag ctggaaggcc
     2821 cagcccactt cctcgcccgc cgcgcgcagg gccagcatcg ccgcgcccag cgccacgcag
     2881 gccagcacca cacccgcggc ataggccagc ccctcggcgc gggcctgggc ggcgctttcg
     2941 ccggcgcggg ccaggctcag cgccttcagg ctgaggatgg ggaacacgca gggcatcacg
     3001 ttcagcagca gcccgcccac caccgctgcc agcagcagcc agccaaagcc gccggtgctg
     3061 ccggtgccat cggccagggg cgtgccgccg gtgggcacgg ggccgggccg ggtggtgatg
     3121 gccaggccat cgcccgcgcg gttgaagcgc agcacgcccg gcaggctggc gggcagggct
     3181 gcgttatggg cgcgcgccag ggtgaggatc agcacatcgc ccttgcgggc aaaaccttgc
     3241 ggcgcggcat aatccaccac gccatctgcg gctgggaaga aatgcggcgc ctctatggca
     3301 gcgccggcgg ggaaggggat ggccaggcgc aagtgcgacc cgctggcggc cagctgtgcc
     3361 ggcgcatcga gcggggcggg cagatgcgcg cgccagccat cgaagcgcgg atcggccgcg
     3421 cctgtgccca ccgtcacgct ggtgtgcagg gtggcctctt ccggcacgca gatctgcgcc
     3481 gaacaggcca gccactgcgc cttcagcgcc accggcaggc tgcccgccgc gcccgccggc
     3541 agggtgaagg gcaccagcac cgcataatcg cgctggtaga catggttcat cagcccgccc
     3601 accagcagcg tttccggcac ggtatagagc ggggcggcca cgcgggcgcc tgcgggcagt
     3661 gcccattcca gccgcatggc aaagccggca tcgcccgggt tctgccaata gccgtgccag
     3721 ccggccgccg gcgccatggc gatggccagc aggatcgtct cgccggggcg ggcggggcgt
     3781 tcggccagca gggtggcggt gatgtggggc gcgatggcag gcgttatggc cggctgggcc
     3841 ggcggcacat cggcgcgggc cggggggctc agcgccattg cggcggccag cgccagccac
     3901 tgccacaata gcgcaacagg gcgcagatag cgaaaggggc tcgaaaaatc tgtcatcggg
     3961 tgtctcttgc cgccggccct gctgtggggc ttgccttggc caaggtgccc cgccatcacg
     4021 cctgtgccgc cagtgcggcc aatttgcaag gaatgcccgc gccgtgaccc aagcccctgc
     4081 ccgcccgctc tcccgctctt tcgcgcatcg ccgctggctg gcggggttga tggcgggggt
     4141 gggcctgctg gcggggggcg cgttgcaggc aaagccggtg gcgcatcatg ccgcccatcc
     4201 ggtggcagcg gcggcgcctg cgcccgccac gccgcccaaa ctgctggtgg cgatcgcggt
     4261 ggatcagttc tcggccgatc tcttcgccca gtatcgccag cactacagcg ccggcatggc
     4321 gcgcctgctc accggcgcag tctttcccag cggtttccag agccatgccg ccaccgagac
     4381 ctgcccgggc cattccaccc tgctgaccgg cgtgcacccc gcgcgcagcg gcatcatcgc
     4441 caacaactgg tatgacaccg ccgccgcccg ccccgacaag cgcatctatt gcgccgagga
     4501 cgaccaccac cccgatagcg actcgaagaa ccccatcgtc tcggccgtgc atctgaaagt
     4561 gccgacgctg ggtgagatga tgaaggcgcg ctggcccgaa agccgcaatg tcgccgtttc
     4621 ggtgaaggac cgggcggtga tgatgatggg cggccaccag accgatgccg cctactggct
     4681 ggtggcgcgc ggctttgaaa cctatcacaa ccagccgatc ggcaatgagg cgctggatga
     4741 gaacgccagc atcctcgccc tgctgaacaa gggcgcgccc gccttcacgc cgccggcctg
     4801 gtgcgccccg cgtgatcgcg ccatcaccac ccgcaacggc gccggcgtgg tgccaagccc
     4861 tgccgcgctg gccgatgggc cgacggtggt gggcaagggc cgctttgccc tggatgccgg
     4921 cgatgccgat ggcttcaagg tgtcgccccg ggccgatgcg gccacgctgg atctggcgct
     4981 gcggctggtg cgcgcgatga agctgggcaa gggggcggcg cccgatctgc tctcggtctc
     5041 gctctcggcc accgattaca tcggccatgc ctatggcacc gaggggatgg agatgtgcat
     5101 ccagcagaac cagctcgatc tggcgctggg ggccttcttt gcccagctcg atgcgctggg
     5161 cctcgattat gccgtggtgc tgacggccga ccatggcggg ctcgacatcc ccgagcggct
     5221 ggaagaccag gcagatccgg ccgcccgccg cgcggccgcc ggcctgtcca gctttgccct
     5281 caacgaggac attgccaggg agctgggcct gaccgccttc ctgccccggc gcgaggccga
     5341 tggcacgctg gcgatcgccc agccggccta tcccaaacag ctcgtctatg ccgaatcggc
     5401 ctttggcgac tacttcatca cccatgagct gagcgaggag cagcgccaga aggtgcaggc
     5461 cctgctggtg gccaagctga aggccagccc gcttgtcgcc catgtgtgga cgaaggccga
     5521 gatcgccagc ttccccatcg cccatggcaa cccgcaggac tggacgatgg aagagcgcgt
     5581 gcgcgccagt tgggatgccc agcgttcggg cgatgtgttc agcgtgctga agcgcaccgt
     5641 ggtgcccatc cccgagccgc gccccggcta caccgccacc catggcagcc cgtgggatta
     5701 tgaccggcgc gtgccgatgc tgttctggcg gcgcggctac accggctttg aacagcccca
     5761 gccggtggag acggtggaca ttgcccccag tcttgccgcg ctggtgggcc tggcagagcc
     5821 ggacggcacg tttgacgggc gctgcctcga tcttgatggc ggcgcgaaga acacttgcga
     5881 aggggccgcc gcagtgccat cgcggcattg atggataaca cgcgcgatct ggtcattctc
     5941 gggggcggtc ttgtcggcat gacgctggcg ctggccgctg cccggcaggg tatcacttcc
     6001 catgtggtgg agcgggaaga ccccgccgat ctcaccgccg aaggcgtcga tggccgcgcc
     6061 tcggcgatat cgagcgcgag ctggaacctc tttcgcaaca tcggcctggc agaggcgctg
     6121 accccgctgg gctgcccgat cgacgcgatt gccgtgaccg accagcacaa gcccggccgc
     6181 atcgattttc gccccgatcc gggcgcggaa tcgctgggcc gcatgtatgc caaccgcgat
     6241 atccgcctcg ctctgttcga ggcggcgcgc cacgagccgg ccattgcctg gcacacccat
     6301 gcgcaggtga ccgggcgcga gcgcggcccc catggcgttg ccgccacgct ggccgatggg
     6361 caggtgctga aaggcagcct gctggtgggc gccgagggcc gccgcagccc cacccgcgag
     6421 gaagagggca ttggcctggt tgcctgggat taccgccacc gtgcgatcgt gacggcgctg
     6481 gcccatgaaa agccgcatga caatgtcgcc tgggaaatct tctatgctgc cgggcccttt
     6541 gccctgctgc cgctgaacga cctgcccgag ccgaccgcgc gcggctatcg ccaccgcagc
     6601 gcgctggtgt ggacggtgcc ggaaaaggat gccgccggcg tcaccgccct gtcggatgcc
     6661 actttccttg ccgaagtggc gcggcggatg gagggcctgt ttggcgggat cgagctgatc
     6721 gcccggcgca tggcctatcc gctgacctat cagaacgcca cgcgcatcac cggcgagcgg
     6781 ctggcgctgg tgggcgatgc cgcccatggc atgcacccga tcgccggcca ggggctgaac
     6841 ctgggcctgc gcgatgtggg cgcgctggtg gaagtgctgg ccgatggcat gcggctgggg
     6901 ctggagccgg gcgatgcgca attgctcaag cgctatgaga actggcgcgc gctcgatatt
     6961 ttcagcacga tgagcgtgat ggatggcatc gtgcggctgt ttggcgtgcc ggggcgcaag
     7021 gcttcggcca ttcgccggct gggcatggca ggggtgcagc gcatgccgct gctcaagcgc
     7081 ttcttcatgg atgaggcgcg cggcctttcg ggccatctgc cggcgctgct gcgcgcggct
     7141 tgaaggctgt tgctggcccg gcctattttg ccgggccggt ggcggcgggc gccggcgttc
     7201 tggcgggcag cggggcagag ctggccgcag ctgccggggc tggcggcgtt gcgggcgcct
     7261 catccggctc ggcgccggca tcgggcgggg gcagggccag cgggctgggc tgcaccaccc
     7321 ggttgccgct attgtcgcgc agggtgcgcg gctccagcac ggcggtcgtc tcgatcaggt
     7381 cgagcgcgcg ttccaccgcg atgtagcgcc gcgcgccggc gatccagccc tgcgctgccg
     7441 ccgcgctgcc gggcatgcgc tccacatcgg cgatggcatc ttccacccgg ccctcgcgca
     7501 ggcacatgcg ggcatggtcc agccggaagg cgggatcggg cgcgggcgtg ccggcttcgc
     7561 ggcgcagcac gaagagattg gccagctcgc gctgcagccg cgtccacacg ctttcgtcgg
     7621 acgggggatt ggccaggcgc ggggccatgg catccagctg ggctgccagc cggtcgagcg
     7681 tgacgggctg gcgggcggtg gcgatgatgg tggcgaccgc ttccggctgg gcgccgccga
     7741 aacgcagctt cagctggtct tccagatagc ccagcgtggc gccgcgttcc accacccggc
     7801 gggcggcaaa ggccaccagc agcgcttcgg cgcgggcggc attgccttcg gccgccgccg
     7861 attccagatc gagccgggca aggcgctgct ccagcgcgga cacgcgccat tccaccgccc
     7921 cttgcgggcc cggcgcggac gctgcgggca ggggggcggg cgcggccagc ggcgcaggcg
     7981 ccacggccag ccggcccggc accagcccca gcccccagcc cagcgccacc acgccgccgc
     8041 tgcccagcac aaagcccagc ccggccatca ccatcagcgc gcgccagcgc cggcggcgca
     8101 gcaccttgcg tgtctggttg ggtaaatcct cggccatgcg cttgcgtcta tccctctcgc
     8161 cctatccgcg ctgaataaga gcgggattct ggcaggtttg caaggccagc gccagcaggg
     8221 cggcatcctc tgcccggtgg gcaacgagaa tatcgcgcca gccctcgccg gcggccgtgg
     8281 ccacgcggcg gccgatggtg gccagcacga tccgccggcg gtcaacgccc agccgcgcgc
     8341 attccgccgc gaaatggcgg gccgcggcag ccgaatgcag cgccaccacc gcgcccggca
     8401 gcgcccgcgc ctgcagcaac agcgccaccg ggccagccag cggcagcgcc gccagccggt
     8461 agaccacctg ctctgccagc gccacgccgg ggggcggggt cagttccacc cgctcttccc
     8521 cggccaggcg cagcaggcgg cgatgggcag gcgccagatg cgccagcagc gcttgcagcc
     8581 ccccttcgcc caccgccacc acgtcgagcc cggccgcccg cgcggcctcg gcagtggccg
     8641 cgcccacggc ataggcgggc ttgccggcaa aggcggccag ctgggggccg gcatggcgca
     8701 gggcattggc gctgcccagc agcagcgcat ccaccgccgc cggcgcaggg gcggcccagg
     8761 ccacaccggc aaccgcgaac agcggcgctg ccaccacctc caccccgggc gccatggcat
     8821 gggcggccgc cgcgctggcg gccagccccg gctccgggcg cagcaggatc agcggcaggc
     8881 tcacgcgggg atcgggtgcg ggccg
//
LOCUS       DASRYE010000052         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_109134,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000052 DASRYE010000000
VERSION     DASRYE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109134"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /gene="ftsA"
                     /locus_tag="VFF98_02950"
     CDS             <1..1033
                     /gene="ftsA"
                     /locus_tag="VFF98_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZU62616.1"
                     /translation="AGAGLASQIAQVELPIGGRRVEPEDIRHLLLAARDVIQPEGRGV
                     LQAQPAHYTLDGAHGVADPQGLHAQSLGVDIHVLMADAAPLRNLTESVQNAHLQVEEV
                     VGSPVAAGIACLSEEERELGVALVEFGAEVTSVSLFAGGMLVGLACLPSGSGDITDAI
                     ASSFGIRRFQAERLKCVNGSAIATPADHREMIPIAAPGDEAPPPASLHADERGRISRA
                     DLIAVITSRLGLLMEEINKALKAMGYTGVRGAQVVFTGGGAELAGLAEFAQGALGRPV
                     RIGRPVPVKGLPEAHTTPGYSALVGLVLQAAAQPLDIRRLAPPRAVAGERGLQGQARR
                     ILRALRDYF"
     gene            1172..2992
                     /gene="ftsZ"
                     /locus_tag="VFF98_02955"
     CDS             1172..2992
                     /gene="ftsZ"
                     /locus_tag="VFF98_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZU62617.1"
                     /translation="MSINIGPPAVEELRPRITVIGVGGAGGNAIANMIRAEIEGVDFI
                     VANTDAQALNSSIASTRIQLGPHITQGLGAGSRPEVGRAAAEETIAEIEAMLDGVHMC
                     FIAAGMGGGTGTGAAPVIAKMARDRGVLTVGVVTKPFLFEGTRRMRSAEAGIAELQKH
                     VDTLIVIPNQNLFLVAKAETTFKEAFLLADEVLQQGVRSITDLMVMPGLINLDFADVR
                     SVMAEMGKAMMGTGEGEGQNRARDAAEKAIANPLLDGVSMQGAKGVIISIIGGEDMRL
                     MEVDEAANHIRELVDPNANIIWGSAFNPDLEGRIRVSVVATGIEQVASSAEDTARPFN
                     MGAARGPARPAAAPASGPATPAPGYAAPAPGFAPQAPGYAAPSVPETLATPAPSILPP
                     AAPIAAAPGHDEEDPFPSPAAFAARAQAAAYAPQPAPVPRPYLGEQAEPFPAPPQQGG
                     PQVSYGFTPPPAYGAPPAEPLDLGAGHEVPGQGPADRAAQDELLLDVSRLIEEDGHVP
                     SAHGAAPGQGRTRGLASFTQNDGAQGGYGADPRGGMGAAPVPPVAGQGAAPAGGSGGT
                     LFERMANLTHRRRAAEVDEPESEDGPSINIPRFLGRQNNQ"
     gene            3040..>4355
                     /locus_tag="VFF98_02960"
     CDS             3040..>4355
                     /locus_tag="VFF98_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU62618.1"
                     /translation="MVARWIWNRPAAHPAAWAAGAAAGALGGLMMAGPAALAQGLPAG
                     VQGGAAAQGGGSYAVVQPLPVAADPGHALDAALSRLARNPLDPGALVAAGDAALAMGD
                     SDAAIGFYTRAERVAPGNAHARAGLAGARLRAGEPLEAVDLFDRAARDGALSGDQIAD
                     RGLAYDMLGDNGSAQRFYREALANGAGDEAVRRLALSQAMAGDARAVEITLAPLLQRQ
                     DRAAWRTKAFAFAILGREEEAVAVARSTMPTQLAEAIAPYLRFMRRLTPAQQAAAANL
                     GRFPRASDIGQDDPKIAAWAAAHGVKRLALAEASPAAGPPRPEAPAPDKARKPRRPRP
                     ATLPEDADSAPGEPTPSREEPALPPAGAAAAATPAPQPAYPPAPAFSLAPAAPRAPAQ
                     PTVALPPPAAPAPAQPAAAGARQATAFPIVQRRPQAAPASAPVSAPA"
BASE COUNT          611 a         1523 c         1621 g          600 t
ORIGIN      
        1 ggcgggcgcc ggccttgcca gccagatcgc ccaggtggag ctgccgatcg gcgggcgccg
       61 ggtggagccg gaggatatcc gccacctgct gctggccgcg cgcgatgtga tccagcccga
      121 ggggcgcggc gtgctgcagg cgcagccggc gcattacacg ctggatggcg cccatggcgt
      181 ggccgatccg caagggctgc atgcgcagag cctgggggtg gatatccatg tgctgatggc
      241 cgatgccgcc cctttgcgca atctgaccga aagcgtgcag aatgcccatc tgcaggtgga
      301 agaggtggtg ggcagcccgg tggccgccgg cattgcctgc ctcagcgagg aagagcgcga
      361 actgggcgtg gcgctggtgg aatttggcgc ggaagtgacg agcgtgtcgc tgtttgccgg
      421 cggcatgctg gtggggctgg cctgcttgcc gtcgggttcg ggcgatatca ccgatgcgat
      481 cgcctccagc ttcggcatcc gccgcttcca ggccgaacgg ctgaaatgcg tgaacggttc
      541 ggcgattgca acgccggctg atcaccgcga gatgatcccc attgccgcgc ccggcgatga
      601 agcgccgccg cccgccagcc tccatgccga cgagcgcggc cgcatctcgc gcgcggatct
      661 gatcgcggtg atcaccagcc ggctggggct gctgatggaa gagatcaaca aggcgctcaa
      721 agccatgggc tataccggcg tgcgcggcgc tcaggtggtg ttcaccggcg gcggggcaga
      781 gctggccggg ctggccgaat ttgcgcaagg ggcgctgggg cggccggtgc gcattggccg
      841 gccggtgccg gtgaagggcc tgcccgaggc gcatacgact ccgggttatt cggcgctggt
      901 ggggctggtg ttgcaggccg ccgcccagcc gctcgacatt cgccggctgg cgcccccgcg
      961 ggcggtggcg ggcgaacgcg ggctgcaggg gcaggccagg cgtatcctgc gggcactgcg
     1021 tgactatttc tgatcttgtg ggcttgccgc gcgggcagct cgcggaaggt ctgtggacaa
     1081 atggcaaaag cgctttgatt agccgattcg aagcgtgcat tttaagagtg aggcggcccg
     1141 cgtccaaagc ggcaagccag aggagaagcg catgagcatc aatatcggtc ccccggctgt
     1201 cgaggagctg cgtccgcgca tcaccgtgat cggggtgggc ggagctggtg gcaacgccat
     1261 tgccaacatg atccgcgccg agatcgaggg tgtcgatttc atcgtcgcca acaccgatgc
     1321 ccaggcgctc aattcctcca tcgcctccac ccgcatccag ctggggccgc acatcaccca
     1381 ggggctgggc gccggatcgc gccccgaagt gggccgcgcc gctgccgagg aaaccatcgc
     1441 cgagatcgag gcgatgctgg atggcgtgca catgtgcttc atcgcggccg gcatgggcgg
     1501 cggcaccggc accggcgctg ccccggtgat cgccaagatg gcgcgcgatc gcggcgtgtt
     1561 gacggtgggc gtggtgacca agccgttcct cttcgagggc acccgccgca tgcgcagcgc
     1621 cgaggccggc attgccgagc tgcaaaagca tgtggacacg ctgatcgtca ttcccaacca
     1681 gaatctcttc ctggtggcaa aggcggaaac cactttcaag gaagccttcc tgctggccga
     1741 tgaagtgctg cagcagggcg tgcgctcgat caccgacctg atggtgatgc ccggcctcat
     1801 caacctcgac tttgccgacg tgcgctcggt gatggccgag atgggcaagg cgatgatggg
     1861 caccggcgag ggcgagggcc agaaccgcgc ccgcgatgcc gccgaaaagg cgatcgccaa
     1921 cccgctgctc gatggcgtgt cgatgcaggg cgccaagggg gtcatcattt ccatcatcgg
     1981 cggcgaggac atgcgcctga tggaagtgga cgaggccgcc aaccacatcc gcgagctggt
     2041 ggaccccaac gccaacatca tctggggcag cgccttcaac ccggatctgg aagggcgcat
     2101 ccgcgtgtcg gtggtggcca ccggcatcga gcaggtcgcc agctctgccg aggataccgc
     2161 ccgcccgttc aacatgggcg ctgcccgggg ccccgcgcgc ccggcggcgg cccccgcatc
     2221 gggcccggca acgcccgcgc ccggctatgc cgcgccggcc ccgggctttg ccccgcaggc
     2281 ccccggctat gccgcgcctt cggtgcccga aacgctggcc acgccggcgc cttccatcct
     2341 gccgccggca gcgcccatcg cggctgcccc cgggcatgat gaggaggatc cgttcccctc
     2401 gccggccgcc tttgccgccc gcgcgcaggc cgccgcatac gcgccgcagc cagcgccggt
     2461 gccgcgcccc tatctgggcg agcaggccga acccttcccc gcgccgcccc agcagggcgg
     2521 gccgcaggtg agctatggtt tcacgccgcc gccggcctat ggcgcgccgc cggccgagcc
     2581 gctggatctg ggcgcgggcc atgaagtgcc ggggcagggc ccggccgatc gcgccgcgca
     2641 ggacgagctg ctgctcgatg tcagccggct gatcgaggaa gatggccatg tgccttcggc
     2701 gcatggcgca gcgccgggcc agggccgcac ccgcggcctt gccagcttca cccagaacga
     2761 tggggcgcag ggcggctatg gcgccgatcc gcgcgggggc atgggcgcag cgccggtgcc
     2821 gccggtggcc ggccagggcg ccgcgccggc gggcggttcg ggcggcacgc tgttcgagcg
     2881 gatggccaat ctcacccatc gccgccgcgc cgccgaagtg gacgagccgg agagcgagga
     2941 tggcccgtcg atcaacattc cgcgttttct cgggcgccag aacaaccagt aaacggcgcg
     3001 agacaacagc ctgacgccgg gcgaggacga aagcggatca tggttgcgag gtggatttgg
     3061 aacaggccgg cggcgcatcc ggcggcctgg gccgcagggg cggcggcagg ggcgctgggc
     3121 gggctgatga tggccggccc ggccgctctg gcgcagggct tgccggctgg cgtgcagggc
     3181 ggcgcggcgg cgcagggcgg cgggtcctat gccgtggtgc agccgctgcc ggtggcggcc
     3241 gatcctggcc atgcgctgga tgcggcgctc tcgcggctgg cgcgcaatcc gcttgatccg
     3301 ggcgcgctgg tggcggcggg cgatgccgcc cttgccatgg gcgacagcga tgcggctatc
     3361 ggcttttaca caagggcaga acgcgtggcg ccgggcaatg cccatgcgcg ggcggggctg
     3421 gcaggcgcac gcctgcgcgc cggcgagccg ctggaagcgg tggacctgtt cgaccgcgcg
     3481 gcgcgcgatg gcgcgctgag cggcgaccag atcgccgatc gcggccttgc ctatgacatg
     3541 ctgggcgaca atggcagcgc ccagcgcttc taccgcgagg cgctggccaa tggtgcgggc
     3601 gacgaggcgg tacgccggct ggcgctgagc caggcgatgg cgggcgatgc gcgcgcggtg
     3661 gagatcaccc tggcgccgct gctgcagcgg caggaccgcg cggcgtggcg caccaaggcc
     3721 tttgcctttg ccattctcgg ccgcgaggaa gaggcggtgg ccgtggcgcg ctccaccatg
     3781 cccacccagc tggccgaggc gattgccccc tatctgcgct tcatgcggcg gttgaccccg
     3841 gcccagcagg ccgcggcggc caatctgggg cggtttccgc gcgcctcgga tatcggccag
     3901 gatgatccga agattgccgc ctgggccgct gcccatggcg tgaagcggct ggccctggcc
     3961 gaggccagcc cggccgccgg cccgccccgg cccgaagccc ccgcgcccga caaggcgcga
     4021 aagccgcgcc gcccccggcc ggccacgctg cccgaggatg ccgatagcgc cccgggcgag
     4081 cccaccccct cgcgcgagga gccggcgttg ccgccggccg gcgccgctgc ggcggcaacg
     4141 cccgcgccgc agcccgccta tccgcccgcg cccgctttca gcctggcccc ggccgcgccg
     4201 cgcgcgccgg cccagcccac cgtggccctg ccgcccccgg ccgccccggc gcccgcgcaa
     4261 ccggctgcag ccggggcgcg gcaggccacg gccttcccga ttgtgcagcg ccggccccag
     4321 gcagcgccgg cttcggcgcc ggtttcagcg ccggc
//
LOCUS       DASRYE010000053         6515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_126773,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000053 DASRYE010000000
VERSION     DASRYE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126773"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFF98_02965"
     CDS             complement(<1..813)
                     /locus_tag="VFF98_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62619.1"
                     /translation="MARPAAPYRAVLIACDTPVDRRWSGWLTARLRRFRTPHALMRAG
                     VAQRVAPIVQLFASAQGDLSEAACAILAHCDALVVVCSTATPASRWIASAIHHYRETA
                     GRERIFALLAEGEPDQAFPEALLRRAITREREGGLCETVWEEAEPIAADVRPRADEPR
                     RATLRRALLRTAAGLLGVSFDQLARRERHRRLQRQRLIGGLCAGALAAGAALAWWQWD
                     SRQLKRAYYEAYATHWGMPSGRNRVSVATASHRGGTYLIETRDGLVLRLVRLD"
     gene            complement(819..4001)
                     /locus_tag="VFF98_02970"
     CDS             complement(819..4001)
                     /locus_tag="VFF98_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZU62620.1"
                     /translation="MSEPEGHDFRYSAFVSYRHLLPDRQWARWLVERLEVYRTPRPLI
                     VAGAPQRVGYLFRDDDEIPASSDLSHQIDAALASSRHLIVICSRATPASVWVSKEIDS
                     FVALGRAGHILVLLIDGEAQEAIPANLWPLWQAAQASRTQASRAQASRGGGQPADGPH
                     MVDVRHITDRNGAAAHAALLAIAAILLRQAPAALAHFDDRRRLSRRRLMRGAMAAAGG
                     LLALQGLYYWDQHRIKYRYFTDVTAQWGVPVGLGEITAEQASRRTFSIALAYQGGEVV
                     GAKAVNGAGLPMAVDDPSGLFAKGTAQIWLTRTDHRVTAVELLSATGKRDHTEIYAWQ
                     PDGSALVSLVRSGGGLSGGALGEPAVPTANTMPYFPSTIAQYRLSFNAGGQLTQRLFR
                     NAIGAQAAQEDGSVGYAYAYDGRGYLVLRRTILSAAGGEASLDRAAQLRFHVNAAGLG
                     EETDWLDAAGHPVPGDNGYARLVRQFDRAGNVVAERYTDGAGRPAIDKASGAASVRLE
                     LDGHGLRVVQRYFGTGAEPVRNAISGAFVERYRFDGQGRIAAAFWHDRQDRPMITATG
                     AAGQSYAYDARGNRTDAVFLDAAGQPVAGVAGYVREHAEYDDKGNLVELAYQDGQGRP
                     AANARLGYARTRFAYDERGNAVAIRRFGQDGQPVADANGNALYAMVYDDRNYLAEVHF
                     LGPDGKPFAGPEGVAGIITHFDDQGRLTGVSYVDEQGNAALNSSTGTSFVETEYDERG
                     RPIAYRYYDKDQRPVLSTQSGSAAVEISYNDAAHSRTETFLGLRDEPVAGKSPHCASL
                     TIETDPRGNDVLRRCGDGRGGPMRDELGAAMVRWAYDSHDRMISAFYLDEAGHLARIA
                     AGYAGVLIDLDDAGHPIRRRFVDIDGRAAVNRVTGTAGFDESYDALGHMVSRQFVGLQ
                     GQRAVDRPEGYWALRQKYDARGNAVEIRGLDTDGQPARLQAVGAFVVRFGYDAYNWQN
                     EVRYLDLAGHLMLSMNGQGPAHIIQQRNAWRQETGWLNLDAADRPFMPAAGQERFARA
                     TFTYDPSGHQTAATYFDAAGHRIPAPRAGQ"
     gene            complement(4086..6230)
                     /locus_tag="VFF98_02975"
     CDS             complement(4086..6230)
                     /locus_tag="VFF98_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62621.1"
                     /translation="MSGPVLPDLGFTVAITGHRQLAPERAGPLEVSIAAILADVAAAL
                     EQARMASPLDAQRALRLRLVSGMAEGADQIAMRAAKATAPWRAEAILPFGRAAFLQTF
                     RDPAAGAQALDDLLDAETPVLELADWAWHGHADDDRTHEYWRARRYVTLGQMLVRQAD
                     LVIALWRGTPPEGRGGTAEVVAEARRAGVPILWLHPETGDLRSFVADPESPHQFPIGQ
                     PLEASLPGGGAAIRMAVSNVLPGADRARAAAIRTFLEAERRPLWRKADGSEITGSNAW
                     PYAMMMWALLADRALYKWPFVRARRVPDAAGRQRRWRFCVLEIPFWWTTKLGGAPQAG
                     APDLRPAIAGATAADAPLAPFMRHADAVASRLGHHYRSGYIAIFLLAALAVVLAGGWL
                     VWHDGKPGFVALELGAIGLAVYLWARMAPRGLMRIRGHNTHQRWLDARLVSESLRSVR
                     LLSWIGFAGRRPAEAAAPPDGGHETPRPVWTPWLVNAIAACPPMPQGAFTPARIGLVA
                     RALGHVIEDQRQYHHGNGARLEKLHERLDMVGLGGVLLGVVVSLLFLLMWLVAGHPWM
                     AGDCTPEAVQAVLQRPLNSPGLAGAMHAWAARCESDGDREWLHHWGYAAAFFGSIGPA
                     FAAAMASIRYHGDYERFAKRSEETAAVLARLKMRADDLVRRAGSGEAARQAPAPPLFE
                     DLLELTLDTQDILDKDLLDWRFAYAARPMPLP"
     gene            complement(6323..>6515)
                     /locus_tag="VFF98_02980"
     CDS             complement(6323..>6515)
                     /locus_tag="VFF98_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62622.1"
                     /translation="QLAGIGLGQAFAGGNGFGEFCGIHGCASFVNARNALARQAGAVQ
                     PAAGARRKNGQKWIDPAAR"
BASE COUNT         1076 a         2353 c         2136 g          950 t
ORIGIN      
        1 atccagcctg accagccgca aaaccagccc atcgcgcgtt tcgatcaggt aggtgccgcc
       61 ccggtgagag gcggttgcga ccgacacccg gttgcggcca gatggcatgc cccaatgggt
      121 ggcatacgcc tcgtaatagg cgcgcttcaa ttgccggctg tcccattgcc accaggccag
      181 ggcagcgcct gccgccagcg cgccggcgca cagccccccg atcagccgct ggcgctgcag
      241 ccggcgatgc cgctcgcggc gcgccagctg gtcgaacgac acccccagca gcccggccgc
      301 cgtgcgcagc aaggcccggc gcaaggtggc gcggcggggc tcgtcggcac gcgggcgcac
      361 gtctgcggcg atcggttcgg cctcttccca aacggtttcg caaagccccc cttccctttc
      421 acgggtgatc gcccggcgca gcagggcctc ggggaaagcc tggtccggct cgccctcggc
      481 cagcagggcg aagatccgtt cgcggccagc cgtctcgcga taatggtgga ttgcgctggc
      541 aatccagcgc gaggccggcg tggcggtgga acacaccacc accagcgcat cgcaatgggc
      601 cagaatggcg catgccgcct ccgacagatc gccctgcgcg ctggcaaaca gctgcacaat
      661 cggcgccacc cgttgcgcca ccccggcccg catcagcgca tggggggtgc gaaaacggcg
      721 cagccgggcc gtcagccagc ccgaccaccg gcgatccacc ggcgtgtcgc aggcgatgag
      781 aacagcgcga taaggggcgg cggggcgggc catgcgcgtc actgtcctgc cctgggggcc
      841 gggatccggt gtccggcggc atcgaaatag gttgccgccg tctggtggcc gcttgggtca
      901 tatgtgaagg tcgcgcgggc aaagcgctcc tggccggcgg ccggcatgaa gggccggtcg
      961 gcggcatcga ggttgagcca cccggtttcc tgccgccatg cgttgcgctg ctggatgatg
     1021 tgggcggggc cctgcccgtt catgctcaac atgaggtggc cggccagatc gagatagcgc
     1081 acctcgttct gccagttata cgcatcataa ccgaaccgca cgacaaaggc gccgacagcc
     1141 tgcaaccggg ccggctggcc atctgtatcc aggccgcgga tctccaccgc attgccgcgg
     1201 gcatcgtatt tttgccgcag cgcccaatag ccctccggcc gatcgaccgc gcgctggcct
     1261 tgcaggccaa cgaactggcg cgacaccata tggcccaggg catcgtagct ttcatcgaaa
     1321 ccggcggtgc ctgtgacccg gttgaccgcc gcgcgcccgt cgatatcgac gaaacggcgg
     1381 cggatcgggt ggccggcatc gtcaagatcg atgaggacac cggcatagcc cgccgcaatc
     1441 cgcgccagat ggcccgcctc gtccagatag aaggccgaga tcatgcggtc gtggctgtca
     1501 taggcccagc gcaccatcgc cgcccccagc tcgtcgcgca tggggccgcc gcgcccatcg
     1561 ccgcaacgcc gcagcacatc attgccgcgc ggatccgtct cgatggtcag gctggcgcaa
     1621 tgcgggctct tgccggccac cggctcgtcc cgcaggccca gaaacgtctc cgtccggctg
     1681 tgggcggcat cattgtagct gatctcgacg gcggccgagc ccgactgggt ggacagcacc
     1741 gggcgctggt ccttgtcgta gtaacgatag gcgatggggc gcccgcgctc gtcatattcg
     1801 gtttccacaa aagacgtgcc cgtgctgctg ttgagcgcgg cattgccctg ttcatccaca
     1861 taggagacac ccgtcagccg cccctgatcg tcgaaatggg tgatgatccc ggccacgcct
     1921 tcgggccctg cgaaaggctt gccatcgggg cccagaaaat gcacttctgc cagatagttg
     1981 cgatcgtcat aaaccatcgc gtagagggca ttgccgttgg catcggcaac cggctggcca
     2041 tcctggccga accgccggat cgccaccgca ttgccgcgct cgtcataggc aaagcgggtg
     2101 cgtgcatagc ccagccgggc attggcggcc ggccggccct gcccatcctg ataggccagt
     2161 tcgacgaggt tgcccttgtc gtcatactcg gcatgttcgc gcacatagcc cgccacgccg
     2221 gccaccggct ggccggcggc atcgaggaac actgcatcgg tgcggttgcc gcgcgcgtca
     2281 taggcgtagg actggccggc cgcgcctgtg gcggtgatca tcggccggtc ttgccggtcg
     2341 tgccagaagg cggcggcgat gcggccctgc ccatcgaagc ggtaacgctc gacaaaggcg
     2401 cccgagatgg cgttgcgcac aggttccgcc ccggtgccga aatagcgctg gaccacgcgc
     2461 agcccatgcc cgtccagctc cagccggaca gaggccgcgc cgctggcctt gtcgatggcc
     2521 gggcggcccg cgccatcggt atagcgttcg gccacgacat tgcccgccct gtcgaactgg
     2581 cgcaccagcc gggcatagcc attgtcgccc ggcaccggat ggccggcagc atccagccag
     2641 tcggtctcct cgccgaggcc agccgcgttc acatggaaac gcagctgcgc ggccctgtcg
     2701 agcgatgcct cgccgccagc ggcagacagg atcgtgcgcc gcaagaccag atagccccgg
     2761 ccatcatagg cataggcata gcccaccgag ccatcctcct gcgccgcctg cgccccgatg
     2821 gcattgcgaa acaggcgctg ggtcaattgc ccgccggcgt tgaagctcag ccggtattgg
     2881 gcgatggttg aagggaaata gggcatcgtg ttggcggtgg gcaccgccgg ttcgcccaat
     2941 gccccgccgc tcagcccgcc gcccgatcgc accaggctga caagggccga accatccggc
     3001 tgccaggcat agatttcggt atgatcgcgc ttgccggtgg ccgacagcag ctcgaccgcg
     3061 gtgacgcgat gatcggtgcg ggtcagccag atctgggcgg tacccttggc aaagaggccc
     3121 gagggatcat cgaccgccat cggcaggccc gcgccattga ccgccttggc ccccaccacc
     3181 tcgccgccct gataggccag cgcgatgctg aaggtgcggc gcgatgcctg ttcggcagtg
     3241 atctcgccaa ggcccacagg caccccccac tgcgcggtga catcggtgaa atagcggtat
     3301 ttgatccgat gctgatccca ataataaagg ccctgcaagg ccagcagccc gcctgccgcc
     3361 gccatggccc cacgcatcag gcgccggcgg gagaggcgcc gccggtcgtc gaaatgggca
     3421 agcgcggccg gcgcctgccg caaaaggatc gcggcgatgg ccaggagggc ggcatgggcc
     3481 gcagcgccat tcctgtcggt gatgtgccgc acatcgacca tatgcgggcc atctgccggc
     3541 tgccccccgc cccggctggc ctgcgcccgg ctggcctgcg tccggctggc ctgcgcggcc
     3601 tgccacaggg gccacagatt ggcgggaatg gcctcctgcg cctcgccatc gatgagcagg
     3661 acgaggatgt gccccgcccg gcccagcgcc acgaagctgt cgatctcctt gcttacccag
     3721 accgatgccg gcgtggcgcg cgagcagatg acgatcaggt gccggctgga ggccagcgcc
     3781 gcatcgatct ggtgcgacag atccgaactg gcgggaattt catcgtcatc gcggaacaga
     3841 tagcccaccc gctgcggcgc cccggccaca ataagcgggc gcggcgtgcg atagacttcc
     3901 agccgctcca ccagccatcg cgcccattgc cggtctggca ggagatggcg atagctgaca
     3961 aaagcgctgt agcggaaatc atggccttca ggctcgctca tgccggctcc tgacacgcaa
     4021 ggcggtttca cgcgagcatg ctatcaatcc atgtcaaagc aaagtatatc tgcgtatatc
     4081 acgcatcacg gcagcggcat gggccttgcg gcataggcaa agcgccagtc gagcaggtcc
     4141 ttgtcgagga tatcctgcgt atcgagcgtg agttcgagca ggtcttcgaa gagcggcgga
     4201 gcaggcgcct ggcgggccgc ctccccggat ccggcgcggc gcaccagatc atccgcccgc
     4261 atcttcagcc gcgccagcac ggcggcggtc tcctccgatc ttttggcgaa acgctcgtaa
     4321 tcgccatgat agcggataga ggccatggcc gcggcaaagg ccgggccgat gctgccgaag
     4381 aaagcggcgg catagcccca atgatgcagc cattcgcggt cgccatcgct ctcgcaccgg
     4441 gccgcccatg cgtgcatcgc cccggccagg cccgggctgt tcagggggcg ctgcagcacg
     4501 gcctgcacgg cttcgggcgt gcaatcgccc gccatccacg gatgccccgc caccagccac
     4561 atcagcagaa acagcagcga gacgaccacc cccagcagaa cgccccccag gcccaccatg
     4621 tccagccgct catgcagctt ttccagccgg gccccgttgc catggtggta ttggcgctga
     4681 tcctcgatga cgtggcccag cgcccgcgcc accagcccga tccgcgccgg cgtgaaggcg
     4741 ccctgcggca tcggcgggca ggcggcaatg gcgttgacca gccatggcgt ccacaccggg
     4801 cggggcgttt catgtccgcc atcgggcggg gcggccgcct cggcggggcg gcgcccggca
     4861 aagccgatcc agctcagcag gcggacactg cgcaggcttt ccgataccag ccgcgcatcc
     4921 agccagcgct gatgcgtgtt gtgccccctg atgcgcatca gcccgcgcgg cgccatgcgc
     4981 gcccacagat agaccgccag cccgatcgcc cccagctcca gcgccacgaa ccccggcttg
     5041 ccatcatgcc agaccagcca ccccccggcc agcaccaccg ccagcgccgc cagcaggaaa
     5101 atggcgatat agcccgagcg atagtggtgc cccagccggc tggccaccgc atcggcatgg
     5161 cgcatgaaag gcgccagcgg ggcatctgcc gccgttgccc cggcgatggc cgggcgcaga
     5221 tcgggcgcgc ccgcctgagg cgcgccgccc agcttggtgg tccaccagaa ggggatttcc
     5281 agcacgcaga agcgccaccg ccgctggcgc ccggccgcat cgggcacccg ccgcgcccgc
     5341 acgaagggcc atttatacag cgccctgtcg gccagcagcg cccacatcat catcgcatag
     5401 ggccaggcat tgctgccggt gatctcgctg ccatccgcct tgcgccagag cgggcggcgc
     5461 tcggcctcca ggaaggtgcg gatggccgcg gcgcgggccc ggtccgcccc cggcagcacg
     5521 ttgctcaccg ccatgcggat ggccgcccca ccgccgggca gcgatgcttc cagcggctgg
     5581 ccgatgggga attgatgcgg gctttccgga tctgccacaa agctgcgcag atcgcccgtt
     5641 tcggggtgca gccacaggat cggcacgcct gcgcggcgcg cctcggccac cacttcggcg
     5701 gtgccgccgc gcccctcggg cggggtgcca cgccacaggg cgatcaccag atccgcctgc
     5761 cgcaccagca tctgccccag cgtgacatac cgccgcgcgc gccagtattc gtgggtgcgg
     5821 tcatcgtcgg catggccgtg ccaggcccaa tcggccagtt ccagcaccgg cgtttcggca
     5881 tcgagcagat cgtccagcgc ctgcgcgcct gcggccggat cccggaaggt ttgcaggaag
     5941 gccgcgcggc caaagggcag gatcgcctcg gcccgccatg gcgcggtggc tttggccgcg
     6001 cgcatggcga tctggtctgc cccctcggcc atgccggaaa ccagccgcag ccgcagcgcg
     6061 cgctgggcat ccagcgggct ggccatgcgg gcctgctcca gcgcggcggc cacatcggcc
     6121 aggatcgcgg caatactcac ttccagcggc ccggcccgtt cgggtgccag ctggcggtgc
     6181 ccggtgatcg ccacggtgaa gccaagatcg ggcagcacag gcccgctcat gcccgcacgc
     6241 ccggtgccca ccgccacttt gccccgttat tcatgcagat atccgccaca gaatcccccc
     6301 tcggaccgcc cggcaaggtg cctcaccggg cggcagggtc aatccatttt tgcccgtttt
     6361 tacgccgcgc gccagccgca ggctggacgg cgcctgcctg ccgcgctagc gcgttgcgcg
     6421 cattcacgaa ggacgcacag ccatgaatac cacagaactc gccgaagccg ttgccgccag
     6481 cgaaggcctg accaaggccg atgcccgcaa gctgg
//
LOCUS       DASRYE010000054        12321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_197569,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000054 DASRYE010000000
VERSION     DASRYE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197569"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFF98_02985"
     CDS             complement(<1..594)
                     /locus_tag="VFF98_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZU62623.1"
                     /translation="MIKKQFALPLEPAGRAGRIVIGPANEAALAALQAPGNWPFHTAI
                     LFGPARSGKSLLAGWFAAQGLGEAVDDAPSMDEDALFHRWNRAQASGTPLLLTAALQQ
                     GPAPEGVRLLGGCWPVRLPDLGSRLGAALILKIGEPDDTMLAELILAHAEARSLPLAE
                     EAAFYLASRMERHHLDAERVVAVIDRLSLERKAPPAMG"
     gene            complement(606..1952)
                     /locus_tag="VFF98_02990"
     CDS             complement(606..1952)
                     /locus_tag="VFF98_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZU62624.1"
                     /translation="MTQLNASSAPRTALFHQAFALRSRRWLAGAGLAALALAGGIALV
                     GPARLIAQIEGERGIAPVVATGDIEVNGVEVEATGKTGTEARDAGWHQAYEKAWAQVH
                     GPAMGGSALESLVSSVVVEQEQIGPHRYHARLGIVFDRGRAGAYLSDNGAAVRRSAPM
                     LLVPVLYSGGVAQVYEVRGPWQRAWAQFRTGASAIDYVRPNGGGGDSLLLNAGQPSRR
                     SRLWWRDILDQFGASDVLLAEARLERQWPGGPVNASFTARFGPDNQALGSFTLTAPDE
                     AGVPAMLAQAVQRCDQIYTDALNRGQLRPDPTLSLEHPQIDPGLARLIAMGRAAEAQA
                     AAAAAQAAAAFDPLTGAAVPSAPPPEARPEARPSAAAVGVVLQFPTPDARSVDGALAA
                     VRAIGGVQSASIASIALGGSSVMRVNYGGNPADFAAALKAHGWQVSGSGAALRIRR"
     gene            2042..3148
                     /gene="purM"
                     /locus_tag="VFF98_02995"
     CDS             2042..3148
                     /gene="purM"
                     /locus_tag="VFF98_02995"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZU62625.1"
                     /translation="MSEKNETPASYTYEHSGVSIAAGNALVKAIGPLAKSTARPGADA
                     ELGGFGGFFDLKAAGYKDPLLVAGNDGVGTKVKLAIDHDRHDRIGIDLVAMCVNDLIV
                     QGAEPLFFLDYFATGKLENGVAARVVAGIAEGCRMAGCALIGGETAEMPGMYAPGDYD
                     LAGFCVGAVERGEQLTGDKVAEGDVLLGLASSGVHSNGYSLVRRLAADKGWKLDRPAL
                     FDNERLLIDYLIEPTRIYVKTLLPFIRAGRIHALAHITGGGLLENVPRVLPEGLHAHI
                     DADGWQQSRLMAFLQAQGNIEPGEMARTFNCGIGMVLAVAAEEAEALAADLAAAGEQV
                     HRIGTIRAGTRGCTVAGSAETWSARESWEATHNA"
     gene            3141..3722
                     /gene="purN"
                     /locus_tag="VFF98_03000"
     CDS             3141..3722
                     /gene="purN"
                     /locus_tag="VFF98_03000"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZU62626.1"
                     /translation="MPKAKVAVLISGSGTNMAALLYASRAADCPFEIVLVASNNPEAG
                     GLKLAAAEGVATFALPHKGMTRADHDGAMDAAIRAAGAEYVALAGYMRILTPGFVESW
                     AGRMVNIHPSLLPRYKGLHTHSRAIEAGDSHGGVTVHLVTPELDDGPILGQTPVAILP
                     DDTAETLAARVLFAEHQLYAPCLARLVREAGRG"
     gene            complement(3821..4243)
                     /gene="ndk"
                     /locus_tag="VFF98_03005"
     CDS             complement(3821..4243)
                     /gene="ndk"
                     /locus_tag="VFF98_03005"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZU62627.1"
                     /translation="MAVTRTFSIIKPDATRRNLTGAVTAKLEEAGLRVVASKRIHLSK
                     EQAEGFYGVHAERPFFNDLVAFMTSGPVVVQVLEGEDAVKRNRDIMGATNPANAEEGT
                     IRKLFAESIEANSVHGSDSEENAAIEIAFFFKPEEIVG"
     gene            complement(4349..4795)
                     /locus_tag="VFF98_03010"
     CDS             complement(4349..4795)
                     /locus_tag="VFF98_03010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZU62628.1"
                     /translation="MRVDFYLLEQGRPETALPLLAEKALGTGERLLVVAGDGARAEAL
                     SQALWGRRPESFIANGLAGGPADARQPVLLAPDLPEGPAANGAGLVAYADGQWREPPA
                     GTARVLLLFGEEQRGAARDVWRMMKARSGVDLHFWQQQGAGWVKKG"
     gene            complement(4811..6262)
                     /locus_tag="VFF98_03015"
     CDS             complement(4811..6262)
                     /locus_tag="VFF98_03015"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZU62629.1"
                     /translation="MQISFVPAASARLVVLPRGSAGWEADLDPVLVGAGQAQRFAGKG
                     GQIAEAFLPAEAGVVRQALLGVGEESDAARRGLALEKAGAALSARYLTSGEEALALQA
                     AGLSADDVAALLLGLRLRGWRQDAYRTTQKDEEKPSLARIEVVGAPEGAESAWAAAVA
                     VAEGVEFTRTLVTEPANVLYPESFVARAEARYAGTGLALRVLDEADMKALGMGALLGV
                     AQGSVKPPRLLVIQWNGGPAGEPPVALVGKGVTFDTGGISLKPAGGMEDMKWDMGGAG
                     AVAGAMLTLALRGARANVVGICGLVENMPDGNAQRPGDVVTSMNGQTIEVINTDAEGR
                     LVLCDALTFVQREYKPKAVVDLATLTGAIIATLAHEYAGIFSTNDEIAAQLVAAGAGV
                     GEELWRLPMGKAYDKMIDSPIADMKNVGGRFGGSITAAQFLARFIEDGVAWAHLDIAG
                     MVWADKPGATWAKGATGYGVRLLDRFIRDTAEG"
     gene            6469..8787
                     /gene="lptD"
                     /locus_tag="VFF98_03020"
     CDS             6469..8787
                     /gene="lptD"
                     /locus_tag="VFF98_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZU62630.1"
                     /translation="MPAPVPAVPIRLLGASALALVASALPVAARAMGANPATGSNVSG
                     LTSAGPSPTDERAPDLPPLGAKAAPASSDPPIYFEANRVDYDHNTDIAVATGDVFLRR
                     NEQTVRADKVTWNRKTGKIHASGHIHAVDRNGNELLTEEMDLTDDLNQGATDAMLLLL
                     REGGRLAASQGVRQADGTILMTHTNYTGCDVVDSKGCPKTPSWQVTAGHVLYDPNRKL
                     LRFKGARLAFFGVRLVPVPGLEIATDGRAMAGFMVPNLHSTSTNGFEYDQTFYAHIGT
                     NRDIAITGSVFTKVQPMAGIHYRALTDTGAYQITGYITKSPVIPQGGTDAQARDMMRG
                     YIDINGRFQLDPNWSVSFSGRAVTDRTFLTRYNITGEDMLRSTVDVERIDANSYFSIA
                     GWNFETLRTGDTQGTQPIALPEIDYRRRLAAKVLGGTVDLQANSLALTRSAGQDTQRA
                     FVSAQWSLRKLTDWGQQITFTALARGDLYHSTGNYLTTTAVYQGAPGWQSRGVVLGAV
                     DVAWPLVGQAFGGTQVLTPRVQLVAAPNTPNLRLPNEDSRAIELEDVNLFALNRYSGY
                     DRIEDGTRITYGLDWHLERPRWRIIANVGQSYRFKDETNLLPDGTGLGGRLSDIVGRT
                     EIRYRDFFAITHRYRLDKDTMAFRRNEVDATIGSDQDYLEVGYVRLNRQLAGELEDLQ
                     DSNEARVATRVAFARYWSMFGSAVVDMSETNASGQKVGAFQPLRTRLGFSYQSDCFEF
                     DADWRRDYVTIGDAYRGSSFELRFVLKNLGFH"
     gene            9011..10315
                     /locus_tag="VFF98_03025"
     CDS             9011..10315
                     /locus_tag="VFF98_03025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU62631.1"
                     /translation="MLAGASALVAAPLSAQEGDSAAQLNLPEHIQMFGKDNPNLRRAT
                     AVINGDIITGTDVNQRLALIVAANGGKIEPDELERLRQQVLRNLIDETLQIQEAKTAD
                     AAVDDAEVEDSYARVAENNFHQDVKALDAYLAKIGSSPASLKRQIRGELSWQRVLRRN
                     VQPFVNVSQEEVKELLDRMKASKGTEEYRLGEIYLSATTDTRATVSDTANRIMQQLKD
                     GGSFVAYARQYSEASTKAVGGDLGWIRLAQLPTELAAAAKNMSAGQLVGPIELKGGFS
                     ILYLIDKRQVLTADPRDAILSLKQISITFPAKTSDAEANKRAAEFAAAIKGAKGCGGV
                     DAVAAKVGAQVVANDAVKARDLPAQLQSSLLALSLGEATPPFGSVNEGVRMLMLCGRD
                     DPKADNGPSFDQLMSQLEDDRVNKRAQMYLRDLRRDAIIEYN"
     gene            10326..11342
                     /gene="pdxA"
                     /locus_tag="VFF98_03030"
     CDS             10326..11342
                     /gene="pdxA"
                     /locus_tag="VFF98_03030"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZU62632.1"
                     /translation="MGPLILSLGDPAGVGPELILAAWRQRGTGTDTGTGGLPFAVAGG
                     ASVLEQAAAGGVPVAAIASLAEAAAVFDHALPVLALPDEPPAGWTPGAPCAAGARLAL
                     ASLQAGAQLAIAGKAAALVTGPIAKSHLARVGFAFPGQTECVADACGVAAQDAVMMLA
                     GPSLRTVPITVHVALSAVPGLLSAALIERRAAIVAEALTRDFGIARPRLAICGLNPHA
                     GEDGRMGDEESRLIAPAIAALQARGLAATGPHPADALFAPRHRASFDVALCMYHDQAL
                     IPLKTLDFDRGVNVTLGLPIIRTSPDHGTAFAIAGKGLADAGATIAAIAMAADCARRR
                     AAAR"
     gene            11339..12181
                     /gene="rsmA"
                     /locus_tag="VFF98_03035"
     CDS             11339..12181
                     /gene="rsmA"
                     /locus_tag="VFF98_03035"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZU62633.1"
                     /translation="MSQPNLPPPNLPPLREVIAAHGLSAAKALGQNFLLDEQLLARIA
                     AIPGQLAGQDVLEVGPGPGGLTRALLRAGARVTAIEMDRRCLPALAELAEAFPGQLTV
                     IEGDATKIDHAALFAGPYAILANLPYNVGTQLFTGWLGGESWPPRWRSLTLMFQQEVA
                     ERIVAASGSDAYGRLAVLAQWRARPKIAMKVHRSAFTPPPKVMSAIVHVVPAEAPEGI
                     SARRLERITEAAFGQRRKMLRQSLKGLPGALDALDALGIDTARRAETLSIAEFTAIAR
                     QMGS"
BASE COUNT         1983 a         4422 c         4104 g         1812 t
ORIGIN      
        1 gcccatcgcc ggcggcgcct tgcgctccag gctcagccgg tcgatcaccg ccaccacccg
       61 ttcggcatcc agatggtgcc tttccatccg gctggcaaga tagaaggcag cctcttcggc
      121 caaaggcagg ctgcgcgcct cggcatgggc gaggatcagc tcggccagca tggtatcgtc
      181 gggctcgcca attttgagga tcagcgccgc gcccagccgc gagccgagat cgggcaggcg
      241 caccggccag cagccgccca gcagccgcac gccctccggc gccgggccct gctgcagcgc
      301 cgccgtcaac agcagcggcg tgcccgaggc ctgcgcccgg ttccagcggt ggaacagcgc
      361 atcctcgtcc atcgacgggg catcatccac tgcctcgccc agcccttgcg cggcaaacca
      421 gccggccagc aggcttttgc ccgatcgcgc cgggccgaac aggatggcgg tgtgaaacgg
      481 ccagttgccc ggcgcctgca gcgcggccag cgccgcctca ttggccggcc cgataacgat
      541 gcgcccggcc cggcccgccg gttcgagcgg cagtgcgaat tgcttcttga tcatgagtct
      601 gtgtcctagc gcctgatccg cagggccgcg cccgatccgc tcacctgcca gccatgggcc
      661 ttgagcgctg cggcaaaatc ggccgggttg ccgccataat tcacccgcat caccgaactg
      721 ccgcccagcg cgatgctggc gatgctggcc gattgcacgc cgccaatggc gcgcacggcc
      781 gccagcgccc catccaccga gcgggcatcg ggcgtgggaa actgcaacac cacgcccacc
      841 gcggctgccg atggccgcgc ctcgggccgg gcttccggcg ggggggcgga tggcacggca
      901 gcgccggtca gcggatcgaa ggcggcggcg gcctgggcgg cagcggcagc cgcctgcgcc
      961 tcggccgcgc ggcccatggc gatcaaccgg gcaaggccgg ggtcgatctg cggatgctcc
     1021 aggctcagcg tcgggtcggg gcgcagctgg ccacggttca gcgcgtcggt atagatctgg
     1081 tcgcagcgct gcacggcctg ggccagcatc gccggcacgc cggcctcgtc gggcgcggtg
     1141 agggtgaagc tgccgagcgc ctgattatcc ggcccgaacc gcgcggtgaa gctggcgttg
     1201 accgggccac cgggccattg ccgctccagc cgggcctcgg ccagcagcac gtccgacgcg
     1261 ccgaactgat cgaggatatc gcgccaccac aggcggctgc ggcgcgaggg ctggccggca
     1321 ttgagcagca gcgaatcgcc cccgccgcca ttgggccgca cataatcgat agcgctggcc
     1381 ccggtgcgga actgcgccca ggcgcgctgc cagggcccgc gcacttcata cacctgcgcc
     1441 accccgcccg aatagagcac cggcaccagc agcatcggcg ccgagcggcg caccgcggcg
     1501 ccattgtccg acagataggc ccccgcccgc ccgcgatcaa acacgatgcc cagccgggcg
     1561 tgatagcgat gcgggccgat ctgctcctgc tccaccacca ccgaagaaac caggctttcc
     1621 aaggcgctgc cgcccatggc ggggccgtgc acctgcgccc aggccttctc ataggcctgg
     1681 tgccagccag cgtcgcgcgc ttcggtgccg gtcttgccgg tggcctccac ttccacgccg
     1741 ttcacctcga tgtcgccggt ggccaccacc ggggcaatgc cgcgctcgcc ctcgatctgg
     1801 gcgatgaggc gcgccgggcc gaccagcgca atgccgcccg ccagcgccag cgcagccagc
     1861 cccgcccctg ccagccagcg gcgggaacgg agcgcaaagg cctgatgaaa cagggcggtg
     1921 cgcggggctg aagaggcgtt caactgggtc atggtcaaat ttcgccggcc ttttgcccaa
     1981 gccggcatgg aaatccaagc aggaaagcgc taggcgcgcc aaaagccata cagaaagcgc
     2041 catgtccgag aagaacgaaa cccccgcctc ctacacctac gagcattccg gcgtgtcgat
     2101 cgcggccggc aatgcgctgg tgaaagccat cggccccctt gccaaaagca ccgcgcgccc
     2161 cggcgccgat gccgaactgg gcggttttgg cggctttttc gacctgaaag cagccggcta
     2221 caaggatccg ctgctggtgg ccggcaatga tggcgtgggc accaaggtga agctggcgat
     2281 cgaccacgac cggcacgatc gcatcggcat cgaccttgtc gccatgtgcg tcaacgacct
     2341 catcgtgcag ggcgcggaac cgctgttttt cctcgattac tttgccaccg gcaagctgga
     2401 gaacggcgtt gccgcgcgag tcgtggcggg cattgccgag ggctgccgga tggccggctg
     2461 cgcgctgatc ggcggcgaga cggcggaaat gccgggcatg tatgcgccgg gcgactatga
     2521 tctggccggc ttctgcgtgg gcgcggtgga acgcggcgag cagctgaccg gcgacaaggt
     2581 ggcagagggc gatgtgctgc tgggcctggc gtcctcgggc gtccattcca acggctattc
     2641 gctggtgcgc cgcctcgcgg ccgacaaggg ctggaagctc gaccgcccgg ccctgttcga
     2701 caatgagcgc ctgctgatcg actatctgat cgagccgacg cgcatttatg ttaaaacgct
     2761 gctgcccttc atccgcgccg ggcgcatcca tgcgctggcc catatcaccg gcggcggcct
     2821 gctcgaaaac gtgccgcgcg tgctgcccga gggcctgcac gcccatatcg acgccgatgg
     2881 ctggcagcag agccggctga tggccttcct gcaggcgcag ggcaatatcg agccgggcga
     2941 gatggcccgc accttcaatt gcggcatcgg catggtgctg gccgtggcgg ccgaggaagc
     3001 cgaggcgctg gccgcagatc tggccgccgc cggcgagcag gtgcaccgca ttggcaccat
     3061 ccgcgccggc acgcgcggct gcaccgttgc cggcagcgcc gagacctgga gcgcgcgcga
     3121 aagctgggaa gccacccata atgcctaagg cgaaggttgc ggtgctgatc tcgggcagcg
     3181 gcaccaatat ggcggcactg ctctatgcca gccgcgccgc cgattgcccc ttcgagatcg
     3241 tgctggtggc cagcaacaac ccggaggccg gcggcctgaa actggccgcg gccgaaggcg
     3301 tggccacctt cgccctgccc cacaagggca tgacgcgcgc cgatcacgac ggcgcgatgg
     3361 atgccgccat ccgcgccgcc ggcgccgaat atgtggcgct ggccggctat atgcgcattc
     3421 tcacgccggg ctttgtggaa agctgggcgg ggcgcatggt caacatccac cccagcctgc
     3481 tgccgcgcta caagggcctg cacacccaca gccgcgccat cgaggcgggc gacagccatg
     3541 gcggcgtcac cgtccacctc gtcacgcccg agctggatga cgggccgatc ctgggccaga
     3601 cgccggtggc gatcctgccc gatgacacgg ccgaaacgct ggccgcccgc gtgctgtttg
     3661 ccgaacacca gctctatgcg ccctgcctgg cgcggctggt gcgcgaggct ggccggggct
     3721 gaagcgagag ggcccaggcc ctcgcccctt ctcccgcccg caaaacaaaa ccgccggagg
     3781 atcgctcccc cggcggtttt catttgccat cgtggcagga tcagccgacg atttcttccg
     3841 gcttgaagaa gaaggcgatc tcgatcgccg cgttctcttc gctgtccgaa ccgtggaccg
     3901 aattggcctc gatcgattcg gcgaacagct tacggatggt accttcctcg gcgttggcag
     3961 ggttggtggc gcccatgatg tcgcggttgc gcttcacggc gtcttcgcct tccagcacct
     4021 gcaccaccac cgggcccgag gtcatgaagg cgaccaggtc gttgaagaag gggcgctcgg
     4081 cgtgcacgcc atagaagccc tcggcctgtt ccttgctgag gtggatgcgc ttggaagcaa
     4141 ccacgcgcag gcccgcttct tccagcttgg cggtcaccgc gccggtgagg ttgcggcggg
     4201 tggcgtcggg cttgatgatc gagaaggtgc gggtaaccgc catggggtat tcctctgcaa
     4261 aaagggggtt gcgtatgggc gcgcgcccta gcggggaaat cggccagcgg caagggcggg
     4321 aaaggtcgta tccggcccca ggcccggctt atcccttctt cacccagccg gcgccctgct
     4381 gctgccagaa atgcagatcc accccgctgc gcgccttcat catgcgccac acatcgcgcg
     4441 cggccccgcg ctgctcctcg ccaaaaagca gcagcacgcg ggcagtgccg gcgggcggct
     4501 cgcgccactg gccatcggca taggcaacca ggcccgcgcc attggccgcc ggcccctcgg
     4561 gcagatcggg cgccagcagc accggctggc gggcatcggc cggcccaccg gccaggccgt
     4621 tggcgatgaa gctttccggc cggcgccccc acagcgcctg cgacagcgcc tcggcccggg
     4681 cgccatcgcc tgccaccacc agcagccgct cgcccgtgcc cagcgccttt tcggccagca
     4741 gcggcagggc ggtttcgggc cgcccctgct ccagcagata gaaatccacc cgcacgggcc
     4801 ctgccctgcc tcagccttcg gccgtgtcgc ggatgaaacg gtcgagcagg cgcacgccat
     4861 agccggtggc gcccttggcc caggtggcgc cgggcttgtc ggcccacacc atgccggcga
     4921 tatcgagatg cgcccaggcc acgccatcct cgatgaagcg ggccaggaac tgcgccgcgg
     4981 tgatcgagcc gccaaagcgc ccgcccacat tcttcatgtc ggcaatcggg ctgtcgatca
     5041 tcttgtcata ggccttgccc atcggcaggc gccacaattc ctcgccaacg ccggcgccgg
     5101 cggcaacaag ctgcgcggcg atctcgtcgt tggtggagaa gatgccggca tattcatggg
     5161 ccagcgtggc gatgatggcg ccggtcaacg tggcgagatc caccacggcc ttgggcttgt
     5221 attcgcgctg cacgaaggtc agcgcgtcgc acagcaccag gcggccttcg gcatcggtgt
     5281 tgatcacctc gatggtctgg ccgttcatgc tggtgacgac atcgcccgga cgctgggcat
     5341 tgccatcggg catgttttcc accagcccgc agatgcccac cacattggcc ctggcgccgc
     5401 gcagggccaa tgtgagcatg gcgcccgcca cagcgccggc gccgcccatg tcccacttca
     5461 tgtcttccat gccgccggcc ggcttcaggc tgatgccgcc agtgtcgaaa gtgacaccct
     5521 tgccgacgag ggccaccggc ggctcgcccg ccggcccgcc gttccactgg atgaccagca
     5581 gccgcggcgg cttcaccgag ccctgcgcca cgcccagcag cgcgcccatg cccagcgcct
     5641 tcatgtcggc ctcgtccagc acgcgcaggg caaggccggt gccggcatag cgcgcctcgg
     5701 cccgggccac gaagctttcg gggtagagca cattggcggg ctccgtcacc agcgtgcggg
     5761 tgaattccac gccctcggcc acggccaccg ccgctgccca ggcgctctcg gccccctcgg
     5821 gcgcgcccac tacctcgatc cgggcgagcg agggcttctc ctcgtccttc tgggtggtgc
     5881 gataggcatc ctggcgccag ccgcgcaggc gcaggcccag cagcagcgcc gccacatcat
     5941 cggccgaaag ccccgccgcc tgcagcgcca aagcctcctc gccgctggtg agatagcgcg
     6001 cgctcagcgc cgcgcccgcc ttttccagcg ccaggccccg cctggccgca tcgctctcct
     6061 cgcccacccc cagcagcgcc tggcgcacca cgcccgcctc tgccggcagg aacgcctcgg
     6121 cgatctgccc gcccttgccg gcaaagcgct gcgcctggcc cgcgcccacc agcaccggat
     6181 cgagatccgc ctcccagccg gcagaaccgc gcggcagaac gacaagacgg gcagaagcgg
     6241 cgggcacaaa gctgatctgc atggacactc cacgaactgg gcgcgtggca gggggccagc
     6301 gcgccggaag gcactcgcct tcgccagccc ggcacgatat cgcctgggca aaggcaagca
     6361 aggcggattg cagttaggcg cgggcagtgc aataggcaag ccatgctccc taccttgcgg
     6421 ccccgctggc cagccctcga ttgtcagagc cagcgcggtg ttcgcgccat gcccgcgccc
     6481 gtgccggccg tgccgattcg cctgctgggg gccagcgcgc tggcgctggt ggccagcgcc
     6541 ctgccagtgg ccgcccgcgc catgggcgcc aaccctgcca ccggcagcaa tgtcagcggc
     6601 ctcaccagcg ccggcccctc gcccaccgac gagcgcgcgc ctgatctgcc gccgctgggc
     6661 gccaaggccg cgcccgccag cagcgatccg ccgatctatt tcgaggccaa ccgggtcgat
     6721 tacgaccata acaccgatat tgccgtggcc accggcgatg tgttcctgcg ccgcaacgag
     6781 cagaccgtgc gcgccgacaa ggtgacgtgg aaccgcaaga ccggcaagat ccacgcctcg
     6841 ggccatatcc acgcggtgga tcgcaacggc aacgagctgc tgaccgaaga gatggacctg
     6901 accgacgacc tcaaccaggg ggcgacggat gccatgctgc tgctgctgcg cgaaggcggg
     6961 cggctggccg ccagccaggg cgtgcgccag gccgatggca ccattttgat gacccacacc
     7021 aactacaccg gctgcgatgt ggtggacagc aagggctgcc ccaaaacgcc cagctggcag
     7081 gtcacggcgg gccatgtgct gtatgatccc aaccgcaagc tgctgcgttt caagggcgcg
     7141 cggctggcct tctttggcgt gcggctggtg ccggtgccgg ggctggaaat tgccaccgac
     7201 gggcgggcga tggccggctt catggtgccc aacctgcact ccacctcgac caacggcttt
     7261 gaatacgacc agaccttcta tgcccatatc ggcaccaacc gagacatcgc catcaccgga
     7321 tcggtgttca ccaaggtgca gccgatggcc ggcatccact atcgcgcgct gaccgatacc
     7381 ggcgcctatc agatcaccgg ctacatcacc aagagcccgg tcatcccgca gggcggcacc
     7441 gatgcccagg cccgcgacat gatgcgcggc tatatcgaca tcaacggccg cttccagctc
     7501 gatcccaact ggagcgtcag cttctcgggc cgcgcggtaa ccgaccgcac cttcctcacc
     7561 cgctacaaca tcaccggcga agacatgctg cgctccaccg tcgatgtgga acggatcgat
     7621 gcgaacagct atttctcgat cgccggctgg aatttcgaga ccctgcgcac cggcgacacg
     7681 caaggcaccc agcccatcgc cctgcccgag atcgactatc gccggcggct ggcggccaag
     7741 gtgctgggcg gcacggtgga tctgcaggcc aacagcctcg cgctcacccg cagcgccggg
     7801 caggataccc agcgcgcctt tgtcagcgcg caatggtcgc tgcgcaaact gaccgattgg
     7861 ggccagcaga tcaccttcac cgcccttgcc cgcggcgatc tctatcacag caccggcaac
     7921 tatctcacca ccaccgccgt ctatcagggc gcgcccggct ggcagagccg cggcgtggtg
     7981 ctgggggcgg tggatgtggc ctggccgctg gtggggcagg cgtttggcgg cacgcaggtg
     8041 ctcaccccca gggtgcagct ggtggccgcg cccaacacgc ccaacctgcg cctgcccaac
     8101 gaggattcgc gcgcgatcga actggaggat gtgaacctct tcgcgctcaa ccgctattcc
     8161 ggctatgatc gcatcgagga tggcacgcgc atcacctacg ggctcgactg gcatctggag
     8221 cggccgcgct ggcgcatcat cgccaatgtc ggccagtcct atcgcttcaa ggacgagacc
     8281 aacctgctgc ccgatggcac cggccttggc gggcggctgt cggatatcgt cgggcgcacg
     8341 gaaatccgct accgcgattt cttcgcgatc acccaccgct accggctcga caaggacacc
     8401 atggccttcc gccgcaacga ggtggatgcc accatcggct ccgaccagga ttatctggaa
     8461 gtgggctatg tgcgcctgaa ccgccagctg gccggcgagc tggaagacct gcaggacagc
     8521 aacgaggcgc gcgtggccac gcgcgtggcc tttgcccgct actggtcgat gttcggctcg
     8581 gcggtagtgg atatgagcga gaccaatgcc agcgggcaga aggtgggcgc cttccagccg
     8641 ctgcgcacgc gcctgggctt ctcctaccag tccgactgtt tcgaattcga tgccgactgg
     8701 cggcgcgatt atgtgacgat cggcgacgcc tatcgcggca gcagcttcga attgcgcttc
     8761 gtgctcaaga atctgggctt ccactagggc cgggcgcggc gctccggctc cttttcaggc
     8821 ccgcagccgg cggcgcgccg gcaccattgg cccgcaagat ggcccaaggg gggttccggg
     8881 ggggctcagc ttgggttaag ggccgcagat gcagcctggg gctggccttg gggccccccg
     8941 catacagaac aggatcgatg ccaaggtgat gatgcaattt cgcaagcgcg tgtccgggcg
     9001 ggtgatgctc atgctggccg gcgccagcgc cctggtggcc gccccgctga gcgcccagga
     9061 aggcgacagc gccgcccagc tgaacctgcc cgagcacatc cagatgttcg gcaaggacaa
     9121 ccccaacctg cgccgggcca cggccgtgat caacggcgac atcatcaccg gcaccgatgt
     9181 gaaccagcgc ctggcgctga tcgtggcggc caatggcggc aagatcgagc cggacgagct
     9241 ggagcgcctg cgccagcagg tgctgcgcaa cctgatcgac gagacgctgc agatccagga
     9301 agcgaaaacc gccgatgccg cggtggatga tgccgaggtg gaggattcct atgcccgcgt
     9361 ggcggaaaac aacttccacc aggatgtgaa ggcgctggat gcctatctcg ccaagatcgg
     9421 ctcctcgccc gcctcgctca agcggcagat ccggggcgaa ctgtcgtggc agcgggtgct
     9481 gcggcgcaat gtgcagccct tcgtcaatgt cagccaggaa gaagtgaagg aactgctcga
     9541 ccggatgaag gcctcgaagg gcaccgagga ataccggctg ggcgagatct atctttcggc
     9601 caccaccgac acccgcgcca ccgtttccga caccgccaac cgcatcatgc agcagctgaa
     9661 ggatggcggc agcttcgtgg cctatgcgcg gcaatattcc gaagcctcga ccaaggcggt
     9721 gggcggcgat ctgggctgga tccgcctggc ccagctgccg accgaactgg ccgcggccgc
     9781 caagaacatg tcggccggcc agctggttgg cccgatcgag ctgaagggcg gcttctcgat
     9841 cctctatctg atcgacaagc gccaggtgct gaccgccgat ccgcgcgatg cgatcctctc
     9901 gctcaagcag atctcgatca ccttcccggc caagaccagc gatgccgagg ccaacaagcg
     9961 cgccgccgaa tttgccgctg ccatcaaggg cgccaagggc tgcggcgggg tggatgcggt
    10021 ggccgccaag gtcggcgcgc aggtggtggc caacgatgcc gtgaaggcgc gcgatctgcc
    10081 cgcccagctg caaagctcgc tgctggcgct gtcgctgggc gaggcaacgc cgccgttcgg
    10141 ctcggtgaac gagggcgtgc gcatgctgat gctgtgcggc cgcgacgacc ccaaggccga
    10201 caatggcccc agcttcgacc agctgatgag ccagctggag gatgaccgcg tgaacaagcg
    10261 cgcgcagatg tatctgcgcg acctgcgccg cgacgcgatc atcgaatata actgagccgg
    10321 cgctgatggg cccgctgatc ctctcgctgg gcgatccggc cggcgtgggg ccagagctga
    10381 tcctggccgc ctggcgccag cgcggcactg gcacagatac aggcacaggc ggcctgccct
    10441 ttgccgtggc cggcggcgcc agcgtgctgg aacaggcggc ggccggcggc gtgccggtgg
    10501 ccgccatcgc cagcctggcc gaggcagcgg cggtgtttga ccatgccctg ccggtgctgg
    10561 ccctgcccga tgagccgccc gcggggtgga ccccgggcgc cccttgcgcg gcaggcgcgc
    10621 ggctggcgct ggcctctctg caggccggcg cgcagctggc catcgccggc aaggccgccg
    10681 cgctggtgac agggcccatc gccaagagcc atctggcccg cgtgggcttt gcctttcccg
    10741 ggcagacaga atgcgtggcc gatgcctgcg gcgtggcggc gcaagatgcg gtgatgatgc
    10801 tggccggccc ttcgctgcgc acggtgccga tcaccgtgca tgtggcgctg tcggcggtgc
    10861 cgggcctgct gtcggccgcg ctgatcgagc ggcgcgcggc gatcgtcgcc gaggcgctga
    10921 cgcgcgattt cggcatcgcg cggccgcgcc tcgccatctg cggcctcaac ccccatgccg
    10981 gcgaggatgg ccgcatgggc gatgaggaaa gccgcctgat tgcgcctgcc atcgccgccc
    11041 tgcaagcgcg cggcctggcc gccaccgggc cccacccggc cgatgcgctg tttgccccgc
    11101 gccaccgcgc cagcttcgac gtggccctgt gcatgtatca cgatcaggcg ttgatcccac
    11161 tgaaaacgct ggatttcgac aggggcgtga atgtcacact ggggctgccg atcatccgca
    11221 cctcgcccga tcatggcacg gcctttgcca ttgccggcaa ggggctggcc gatgccggcg
    11281 ccactatcgc ggcgattgcc atggccgccg attgcgcccg ccgccgcgcg gctgcccgat
    11341 gagccagccc aacctgcctc cccccaacct gccccccttg cgtgaagtga tcgccgcgca
    11401 tggcctctct gccgccaagg cgctggggca gaacttcctg ctcgacgagc agttgctggc
    11461 ccggatcgcg gccattcccg gccagctggc cgggcaggat gtgctggaag tgggccccgg
    11521 cccgggcggc ctcacccgcg cgctgctgcg cgccggcgcg cgcgtcaccg ccatcgagat
    11581 ggaccggcgc tgcctgcccg cgctggccga actggccgag gccttccccg gccagctgac
    11641 ggtgatcgag ggcgatgcca ccaagatcga ccatgcggcg ctgtttgccg gcccttatgc
    11701 catcctcgcc aacctgccct ataacgtggg cacgcagctg ttcaccggct ggctgggcgg
    11761 cgaaagctgg ccgccgcgct ggcgctcgct gacgctgatg ttccagcagg aagtggccga
    11821 acgcatcgtg gccgccagcg gcagcgatgc ctatgggcgg ctggccgtgc tggcccagtg
    11881 gcgcgcccgg ccgaagatcg ccatgaaggt gcatcgcagc gccttcaccc cgccgcccaa
    11941 ggtgatgagc gcgatcgtcc atgtggtgcc cgcagaagcg cccgagggga tttccgcccg
    12001 gcggctggag cggatcaccg aggccgcctt cggccagcgc cgcaagatgc tgcggcaaag
    12061 cctgaagggc ctgcccggcg cgctggacgc gctggacgcg ctgggcatcg ataccgcccg
    12121 ccgcgccgaa acgctctcca tcgccgaatt taccgccatc gcccggcaga tgggcagctg
    12181 aaccttcagc gccccagcca gccgagcagt tgcagggccg ccagcgcagc ggccaccgcc
    12241 acgatcgtca tcgtcgtgaa ttcgcgccgc agcgagggcg ccgccacggc aggggcagcg
    12301 gcccgttcgg ccagcggcgc c
//
LOCUS       DASRYE010000055         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_160916,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000055 DASRYE010000000
VERSION     DASRYE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160916"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFF98_03040"
     CDS             <1..791
                     /locus_tag="VFF98_03040"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZU62634.1"
                     /translation="PVHRRAQELGIEVRTPASLKGAEEQAAFAALGADVAVVAAYGLI
                     LPRAVLAAPVHGCLNIHASLLPRWRGAAPIHRAILAGDAETGVGIMQMEAGLDTGPVR
                     AEARTPVAGKTTGELTAELAAMGAGLVCRVLGNLAAFPPVPQPAEGITYAHKVDKAEA
                     RLDFTAGAQQVLRQIRAFMPAPGAFFELEGERYKVLAAEPAAPPMPASQPASQPGTTL
                     DDALTIACADGAIRPTTIQRAGKPAMAVADLLRGRPIPAGTRLS"
     gene            788..1537
                     /gene="truA"
                     /locus_tag="VFF98_03045"
     CDS             788..1537
                     /gene="truA"
                     /locus_tag="VFF98_03045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZU62635.1"
                     /translation="MTRFALTLEFDGTPFMGLQRQAHGPSVQQAVEEAVQRITGEAAV
                     LFAAGRTDAGVHALHMRAHVEIAKPIAPFRLMEALNFHLRPDPIAVLDCQVVADDWHA
                     RFSCIGRSYIYRIVNRRAPLTVDLHRAWQVTQPLDAEAMHEAAQALVGRHDFTTFRSV
                     HCQARSPVKTLDRLEVRREGEEVIIETAARSFLHHQVRSMVGALSFVGLGRWRVAQVG
                     EALAACQRQALPLNAPAAGLYFNRALYPEEA"
     gene            complement(1600..2574)
                     /locus_tag="VFF98_03050"
     CDS             complement(1600..2574)
                     /locus_tag="VFF98_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU62636.1"
                     /translation="MLSENRATTILPAPDIELDVRETFGIDIDMKVPAFSIADERVPD
                     RDDSYVFDPDTTLAILAGFAFNRRVMVQGYHGTGKSTHIEQVAARLNWPCIRINLDAH
                     ISRIDLIGRDAIVLKDGLQVTEFREGLLPWALQTPTALVFDEYDAGRPDVMFVIQRVL
                     ETEGKLTLLDQNRVIRPNPWFRLFATANTVGLGDTSGLYHGTQAINQGQMDRWNIVVG
                     LNYLPGAVETDIVTKKVAGLDPKVAADMVKVADLSRQGFINGDISTVMSPRTVITWAQ
                     NTAIFKDPGFAFRLSFLNKCDETERMLVAEYYQRVFGVDLPESVVAKA"
BASE COUNT          436 a          967 c          965 g          457 t
ORIGIN      
        1 cgcccgtgca ccgccgcgcg caggagctgg gaatagaggt gcgcacgccc gccagcctga
       61 agggagcgga ggaacaggcc gcctttgccg cgctgggggc cgatgtggcg gtggtggccg
      121 cctatgggct gattctgccc cgggccgtgc tggcagcgcc ggtgcatggc tgcctcaaca
      181 tccacgcctc gctgctgccg cgctggcggg gggccgcgcc catccaccgg gcgatcctgg
      241 cgggcgatgc ggaaaccggc gttggcatca tgcagatgga agccgggctg gacaccggcc
      301 cggtgcgggc cgaggcgcgc acccccgtgg ccgggaagac caccggcgag ctgaccgccg
      361 agctggccgc catgggcgcc ggcctcgtct gccgcgtgct gggcaatctg gcggccttcc
      421 cgcccgtgcc ccagccggcc gagggcatca cctatgccca caaggtggac aaggccgagg
      481 cccggctgga tttcaccgcc ggcgcccagc aggtgctgcg ccagatccgc gccttcatgc
      541 ccgcccccgg cgccttcttc gagctggagg gggaacgcta caaggtgctg gcggcagagc
      601 ctgccgcgcc gcccatgccc gctagccagc ccgccagcca gcccggcacc acgctggacg
      661 atgcgctgac catcgcctgc gccgatggcg ccatccggcc gaccaccatc cagcgcgccg
      721 gcaagcccgc catggccgtg gccgatctgc tgcgcggccg ccccatccct gccggcacgc
      781 gcctttcatg acccgctttg ccctgacgct ggaatttgac ggcaccccct tcatgggcct
      841 gcagcggcaa gcccatggcc cctctgtgca gcaggcggtg gaagaggcgg tgcagcgcat
      901 caccggcgag gcggccgtgc tgtttgccgc cgggcgcacc gatgccggcg ttcatgccct
      961 ccacatgcgc gcccatgtgg agatcgccaa gccgatcgcc ccgttccggc tgatggaagc
     1021 gctgaacttc cacctgcgac ccgatcccat cgccgtgctc gattgccagg tggtggccga
     1081 tgactggcac gcccgcttct cctgcatcgg ccgcagctac atctaccgca tcgtcaaccg
     1141 ccgcgcgccg ctgacggtgg acctgcaccg cgcctggcaa gtgacccagc cgctggacgc
     1201 agaggccatg cacgaggccg cgcaggcgct ggtcggccgg catgatttca ccaccttccg
     1261 ctcggtgcac tgccaggcgc gaagcccggt gaaaacgctg gaccggctgg aagtgcgccg
     1321 cgagggcgag gaagtgatca tcgagaccgc cgcgcgcagc ttcctgcacc atcaggtgcg
     1381 ctcgatggtg ggggcgctgt ctttcgtcgg gctggggcgc tggcgggtgg cgcaggtggg
     1441 cgaggcgctg gccgcctgcc agcggcaggc cctgccgctc aacgcgcccg cggccgggct
     1501 ctatttcaac cgggcgttgt atcccgaaga ggcgtgaaag gcctggcccg aagaggcgtg
     1561 aaaggccggg ggcaggcacc ctgcccccgg cctttcccat caggccttgg ccaccacgct
     1621 ttcgggcagg tccacgccga acacgcgctg gtaatattcg gccaccagca tgcgctcggt
     1681 ctcgtcgcac ttgttgagga acgacaggcg gaaggcaaag cccggatcct tgaagatcgc
     1741 ggtgttctgc gcccaggtga tcaccgtgcg cgggctcatc acggtggaga tatcgccatt
     1801 gatgaagccc tggcggctga ggtcggccac cttcaccatg tcggcggcca ccttgggatc
     1861 aaggccggcc accttcttgg tgacgatatc ggtttccacc gccccgggca gatagttcag
     1921 gccaaccacg atgttccagc ggtccatctg gccctggttg atcgcctggg tgccgtgata
     1981 aagcccgctg gtatcgccaa ggcccaccgt gttggcggtg gcgaacaggc ggaaccaggg
     2041 gttggggcgg atcacgcggt tctggtcgag cagggtcagc ttgccctcgg tctccagcac
     2101 gcgctggatg acgaacatca cgtccggccg gccggcgtca tattcgtcga acaccagcgc
     2161 ggtcggcgtc tgcagcgccc agggcagcag gccttcgcga aattccgtca cctgcagccc
     2221 gtccttcagc acgatggcat cgcggccgat gaggtcgata cggctgatat gggcatcgag
     2281 gttgatgcgg atgcacggcc agttgaggcg cgctgccacc tgctcgatgt gagtcgactt
     2341 gccggtgcca tgatagccct gcaccatcac ccggcggttg aaggcaaagc ccgccaggat
     2401 cgccagcgtc gtgtccggat cgaacacata gctgtcgtcg cggtcgggca cgcgctcgtc
     2461 ggcgatggaa aaggccggca ccttcatgtc gatatcgatg ccgaaggtct cgcgcacgtc
     2521 gagctcgatg tcgggcgcgg gcaggatggt ggtggcgcgg ttttcggaaa gcatgggtgt
     2581 ggatcttccg tcgtctaacc ttttcgcgcc tatacgggcc tgcgccgcgc tgcaatatgt
     2641 ttggcacaaa gcctgtcacg aatcgcaggc ccagaacgga tatgaggaga ggcccgtgct
     2701 ggtttatggc gcgctgatat cgccctttgt gcgcaaggtg tgcctggtgg ccgaggaaaa
     2761 gggcgcgccc tggcggctgg aagtgcccaa cctcaaggcc cccgcggccg attttgccgc
     2821 cgcca
//
LOCUS       DASRYE010000056         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_250817,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000056 DASRYE010000000
VERSION     DASRYE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250817"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFF98_03055"
     CDS             complement(<1..1454)
                     /locus_tag="VFF98_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU62637.1"
                     /translation="MAETMGTWPLTIPGAAAAAAAQWPDRPALIVGGQEWSFAQLWAD
                     ARAAASAMLACGLGHGDRIAIWAPNGREWILAALGAQALGAAVVPLNTRFKGPEAADI
                     LRRAQVRLLFAPADFLGQDYARLIAGEDLPDLAETIHTDRAFEAFLARGKGAGDPAVD
                     VALAALAPDDISDIIFTSGTTGRPKGALTAHRQVTQIFGDWAVRVDLRAGDRYLIVNP
                     FFHTFGYKAGWVACLLRGATILPMATFDVAETVRQIEQNRISFIPGPPTIYQMLLQEL
                     EGGARRDFASLRVAVTGAAPVPPALVERMRVELGMQNIVNGYGMTECGCIAMTRQGDD
                     NDTIANTCGYPLPGLEVRCVDGQGQDQPAGTPGELWVRGYGVMRGYLDDPAASAEAVD
                     ADGWLHTGDIGTIDAKGYLNITDRLKDMFISGGFNVYPAEVEKLLSAHPAIAMAAVVG
                     VPDARMGEVAKACVVLRAGARLAEGELAAWSREV"
     gene            complement(1471..2673)
                     /locus_tag="VFF98_03060"
     CDS             complement(1471..2673)
                     /locus_tag="VFF98_03060"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZU62638.1"
                     /translation="MREAAIISTARTGVGKAYRGAFNDTEAPRMAAHVVNAAIERAGI
                     DPARIDDVYMGCANQWNTQSYNLGRLAVHGSVLPDSVAGFAMDRKCSSGLNALAFAAR
                     GIIAGEVDCALAGGTENVSLTIDKHYPNFRNRSEWVKQHDPYAYMAMIETAEIVADRY
                     GISREAQDRFAAQSQQRAAAAQEAGAFAAEIVPIALTKALFDKEGHETGKQELTLDRD
                     EGIRAGTTYEALAGLKPVFRGGQVVQEGRHITAGNASQLSDGASAQVLMDLATAQKEG
                     LPVLGLYRGFQVAGCGADEMGIGPVHAIPKLLDRHGLGIDDIGLWEINEAFASQAIYC
                     QERLGIDPAKLNVNGGGIAIGHPFGMTGSRLVGHALIEGRRRGVRYVVVSMCVAGGMG
                     AAGLFELA"
     gene            complement(2760..3239)
                     /locus_tag="VFF98_03065"
     CDS             complement(2760..3239)
                     /locus_tag="VFF98_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZU62639.1"
                     /translation="MTKIFATPAALLGSEGTQLGPTGWLAIDQARVDGFAGVTGDHQW
                     IHVDVARARQGPFGGTIAHGYLTMSLINHFLPELIEVRGFAHAVNVGLDRLRFLAPVL
                     VGARIRAVSEIVSAEAVKGAIQAIVRITIEIEGPDGQPQPRPALVADAISRYFPEGG"
     gene            complement(3243..4028)
                     /locus_tag="VFF98_03070"
     CDS             complement(3243..4028)
                     /locus_tag="VFF98_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62640.1"
                     /translation="MSVPVPAYPAPLGLLKGKGVVVTAAAGTGIGYAVAKRAAEEGAR
                     LLISDYHERRLEEAADRIAAETGAPRPATCVCDVTAEADVQGLRDAALAALGGVDVLI
                     NNAGLGGEVPVVDMTDEQWSRVLDVTLTSLFRMTRAFLPPMQAAGRGVMVNNASVLGW
                     RAQKGQAHYAAAKAGVMAFTRCAALEAAEHGVRINAVAPSLAMHPFLAKVTSEDVLAR
                     LVEREAYGRPAQVWEVANVMLFLASDLSSYMTGEVVAVSSQQA"
     gene            complement(4025..5203)
                     /locus_tag="VFF98_03075"
     CDS             complement(4025..5203)
                     /locus_tag="VFF98_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62641.1"
                     /translation="MKLGFSAGDEAFRAECADWLNGQMAGEFKDIKGITTLTAKAERR
                     REWEQQLGAHRWGCIGWPAAWGGRDATLAQQVIFAEEYARAGVPGRVNHIGIELAGPT
                     ILAFGTEAQKARFLPGIAAGNTIFCQGFSEPGAGSDLASVRTKGRLEEGEWVVNGQKI
                     WTSLAHISDWIFVVTRTGEGSRGPKGLTFLMMPLNQPGIEVRGITQINGDAEFNETFF
                     TDARCPADSLIGNVGDGWKIAMGLLAFERGVSTLGQQMGFRNELDGIIAAARANGAAG
                     DPLIRQRIARAEIGLRLMRYGALRMLSNTDHARIDGAALTYKIQWASWRRNLGELAMD
                     VLGQAGEVAEGAAYEWGMLPNLFLYSRADTIYGGTNQIQRNLIAERGLGMPREPRGDL
                     "
     gene            complement(5200..>5668)
                     /locus_tag="VFF98_03080"
     CDS             complement(5200..>5668)
                     /locus_tag="VFF98_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU62642.1"
                     /translation="ADPAAALAAARATGWIVAAAEALGGAQACLDRTVAYARERVQFG
                     RPIGSFQAYKHRLADMMIEIEQARSAVYWAACALDEGSDEAPLALHAAKSFAADTFFM
                     CAGNMIQLHGGIGFTWEHDAHLFFKRARALQSMLGNGNWHRERIAGMILGDAA"
BASE COUNT          906 a         2016 c         1859 g          887 t
ORIGIN      
        1 atcacctcgc gtgaccaggc ggccagctcg ccttcggcca ggcgggcgcc ggcgcgcagc
       61 accacgcagg ccttggccac ctcgcccatg cgcgcatccg gcacgcccac caccgctgcc
      121 atggcgatcg ccggatgggc cgagagcagc ttttccacct ctgccggata gacattgaaa
      181 ccgccggaaa tgaacatgtc cttcagccgg tcggtgatgt tgagatagcc tttggcatcg
      241 atcgtgccga tatcgccggt gtgcagccag ccatcggcgt ccactgcctc ggcgctggcg
      301 gccggatcat cgagatagcc gcgcatcacg ccatagccgc gcacccacag ttcgccgggg
      361 gtgccggcgg gctggtcctg cccctgcccg tcgacgcagc gcacctccag ccccggcagc
      421 ggatagccgc aggtgttggc gatggtgtcg ttgtcgtcgc cctggcgggt catggcgatg
      481 cagccgcatt cggtcatgcc atagccgttg acgatgttct gcatgcccag ttccacccgc
      541 atgcgctcca ccagcgccgg cggcaccggg gcggcgcctg ttaccgccac ccgcagcgag
      601 gcaaaatcgc gccgcgcgcc gccctccagc tcctgcagca gcatctggta gatggtgggc
      661 gggccgggga tgaaactgat gcggttctgc tcgatctggc gcacggtttc ggccacgtca
      721 aacgtggcca tcggcaggat cgtggcgccg cgcagcaggc aggccaccca gcccgccttg
      781 tagccgaagg tgtggaagaa ggggttgacg atgagatagc gatcgcccgc ccgcagatcg
      841 acccgcacgg cccaatcacc aaagatctgc gtgacctgcc ggtgcgcggt gagcgcaccc
      901 ttgggccggc cggtggtgcc cgaggtgaag atgatatcgc tgatgtcatc gggcgccagg
      961 gcggccagcg ccacatccac ggccggatcg cccgcaccct tgccgcgcgc cagaaaggcc
     1021 tcgaaggcgc ggtctgtgtg gatggtttcg gccagatcgg gcaggtcctc gccggcgatc
     1081 agccgggcat agtcctgccc caggaaatcg gccggggcaa acagcaggcg cacctgcgcc
     1141 cggcgcagga tatcggccgc ctctggcccc ttgaagcggg tgttgagcgg caccaccgcc
     1201 gcgcccagcg cctgcgcgcc cagcgccgcc aatatccatt cccgcccgtt gggggcccag
     1261 atggcaatgc gatcgccatg gccaaggccg caagccagca tcgccgaggc cgccgcgcgg
     1321 gcatcggccc acagctgggc aaagctccat tcctgcccgc ccacgatcag ggcgggccga
     1381 tccggccatt gcgcggccgc cgctgccgcc gccccgggaa tggtaagcgg ccatgtgccc
     1441 atcgtctcgg ccatcgcgcg tcctttccgc tcaggccagt tcgaacaggc cggccgcgcc
     1501 catgccgccc gccacgcaca tggaaacaac gacatagcgc acgccccgcc gccgcccctc
     1561 gatcagcgca tggccgacaa gccggctgcc ggtcatgccg aagggatggc cgatggcgat
     1621 gccgccgccg ttgacgttga gctttgccgg atcgatcccc agccgctcct ggcaatagat
     1681 cgcctgcgag gcgaaggcct cgttgatttc ccacaggccg atatcatcga tgccgaggcc
     1741 atggcggtcc agcagcttgg ggatggcatg gacagggccg atgcccatct cgtcggcccc
     1801 gcagccggcc acctggaagc cgcgatagag ccccagcacc ggcaggcctt ccttctgcgc
     1861 cgtcgccagg tccatcagca cctgcgccga ggcgccatcc gacagctggc tggcattgcc
     1921 ggcggtgatg tggcgccctt cctgcaccac ctgcccgccc ctgaacaccg gcttcagccc
     1981 ggccagcgcc tcataggtgg tgccggcgcg gatgccctcg tcgcggtcga gcgtcagctc
     2041 ctgcttgccg gtttcatggc cttccttgtc gaacagcgcc ttggtcagcg caatcggcac
     2101 gatctctgcg gcaaaggcgc cggcttcctg ggcggcggcg gcgcgctgct ggctctgcgc
     2161 ggcaaagcgg tcctgcgcct cgcggctgat gccatagcgg tcggccacga tctcggccgt
     2221 ctcgatcatc gccatatagg cgtaaggatc atgctgcttc acccattccg agcgattgcg
     2281 gaaattggga taatgcttgt cgatcgtcag cgagacattc tcggtgccgc cggccagcgc
     2341 gcaatccacc tcgccggcga tgatgccgcg cgcggcaaag gccagcgcgt tcagcccgga
     2401 ggaacatttg cgatccatcg caaagccggc cacgctatcg ggcagcaccg agccatgcac
     2461 ggccagtcgg ccaaggttgt agctctgggt gttccactgg ttggcgcagc ccatatacac
     2521 atcgtcgatg cgggccggat cgatgcccgc ccgctcgatc gcggcattca ccacatgggc
     2581 ggccatgcgg ggggcttcgg tgtcgttgaa ggcaccgcga taggccttgc cgacgccggt
     2641 gcgggcggtg gaaatgatgg ctgcttcgcg catggatgcg ctcctctcag atggtgtggg
     2701 ggtgggcggg gccgcgcagg gccccgcatg gccggaaggc cggccgcaac cggcggggtt
     2761 cagccgcctt cggggaaata acggctgatg gcatcggcca ccagggcggg cctgggctgg
     2821 ggctggccgt ctggcccctc gatctcgatg gtgatgcgca cgatcgcctg gatcgccccc
     2881 ttcacggcct cggccgagac aatctcgctg accgcccgga tgcgcgcgcc caccagcacc
     2941 ggcgccagaa agcgcagccg gtcgagcccg acattcaccg catgggcaaa gccgcgcacc
     3001 tcgatcagct cgggcaggaa atggttgatc aggctcatcg tcagatagcc atgggcgatt
     3061 gtgccgccaa aggggccctg cctggcccgc gccacatcga catggatcca ctggtgatcg
     3121 cccgtcaccc cggcaaagcc atccacccgc gcctggtcga tggccagcca gccggtgggg
     3181 cccagttgcg tgccctcgct gcccagcagg gcggcaggcg ttgcgaagat tttcgtcatc
     3241 gctcaggcct gctggctgga aacggcgaca acctcgccgg tcatgtagct ggacagatcg
     3301 ctggcgagga acagcatcac attggccact tcccacacct gcgccgggcg accataggcc
     3361 tcgcgctcca ccagccgggc cagcacgtcc tcgctggtca ccttggccag gaaggggtgc
     3421 atggccaggc tgggcgccac cgcgttgatg cgcacgccat gctctgccgc ttccagcgcg
     3481 gcgcagcggg tgaaggccat caccccggcc ttggcggcgg cataatgggc ctggcccttt
     3541 tgcgcacgcc agcccagcac cgaggcattg ttgaccatca ccccgcgccc tgccgcctgc
     3601 attggcggca ggaaggcgcg agtcatgcgg aacaggctgg tcagcgtcac gtcgagcacg
     3661 cggctccact gctcgtcggt catgtcgacc accggcacct cgccgccaag gccggcattg
     3721 ttgatcagca catccacccc gcccagcgcc gccagcgccg catcgcgcag gccctgcaca
     3781 tcggcctcgg cggtcacgtc gcacacgcag gtggccgggc gcggggcccc ggtttcggcg
     3841 gcgatgcgat cggccgcctc ctcaaggcgc cgctcgtggt aatcgctgat cagcaggcgc
     3901 gcgccctcct cggcggcgcg tttggccacg gcatagccga tgcccgtgcc ggcagcggcg
     3961 gtgacaacca cccccttgcc cttcagcagg cccagcgggg cgggataggc aggaacagga
     4021 acgctcacag gtcacctcgg ggttcgcggg gcatgccaag gccgcgctcg gcaatcaggt
     4081 tgcgctggat ctggttggtg ccgccataga tcgtgtcggc gcgcgaatag aggaacaggt
     4141 tgggcagcat gccccattca taggccgcgc cctcggccac ttcgccggcc tggcccagca
     4201 catccatcgc cagctcgccc agattgcggc gccagctggc ccactggatc ttgtaggtca
     4261 gcgcagcgcc atcgatgcgg gcatgatcgg tgttcgacag catgcgcagc gcgccatagc
     4321 gcatcagccg caggccgatc tcggcccggg cgatgcgctg gcggatcagc ggatcgcccg
     4381 ccgcgccatt ggccctggcc gccgcgatga tgccatccag ctcgttgcga aagcccatct
     4441 gctggcccag cgtggacacg ccccgctcga aggccagcag gcccatggca atcttccagc
     4501 catcgccgac attgccgatc aggctgtcgg ccgggcagcg ggcatcggtg aagaaggtct
     4561 cgttgaattc agcatcgccg ttgatctggg tgatgccgcg cacctcgatg cccggctggt
     4621 tcagcggcat catcaggaag gtgaggccct tggggcccct ggagccttcc ccggtgcggg
     4681 taaccacaaa gatccagtcg gagatatggg caaggctggt ccagatcttc tggccgttga
     4741 ccacccattc gccctcctcc agccggccct tggtgcgcac gctggcaagg tcggatccgg
     4801 cgccaggctc ggaaaagccc tggcagaaga tcgtgttgcc cgccgcgatg ccgggcagga
     4861 agcgcgcctt ctgcgcctcg gtgccaaagg ccaggatggt gggcccggcc agctcgatgc
     4921 cgatatggtt cacccggccc ggcacgcctg cacgggcata ttcctcggca aagatcacct
     4981 gctgggccaa agtggcatcg cgcccgcccc aagccgccgg ccagccaatg cagccccagc
     5041 gatgggcgcc cagctgctgc tcccattccc ggcgccgctc ggccttggcg gtgagcgtgg
     5101 tgatcccctt gatatccttg aattcgccgg ccatctggcc attcagccaa tcggcgcatt
     5161 cggcgcggaa tgcctcatcc ccggcggaaa agcccagctt catgccgcat ctcccaaaat
     5221 catgcccgcg atgcgctcgc ggtgccagtt gccattgccc agcatcgact gcagcgcgcg
     5281 ggcgcgcttg aagaagaggt gggcgtcatg ctcccaggtg aagccgatgc cgccatgcag
     5341 ctggatcatg ttgcccgcgc acatgaagaa agtgtcggca gcaaagctct tggcggcatg
     5401 cagcgccagc ggcgcctcgt ccgatccctc gtccagcgcg caggccgccc aatagaccgc
     5461 cgagcgcgcc tgctcgatct cgatcatcat gtcggccagg cggtgcttgt aggcctggaa
     5521 gctgccgatc gggcggccga actgcacccg ctcacgcgca taggcaacgg tgcgatcgag
     5581 gcaggcctgc gcgccgccca gcgcttctgc ggcggccacg atccagccgg tggcgcgcgc
     5641 cgcggcaagg gcggcggccg gatcggcc
//
LOCUS       DASRYE010000057        17033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_197667,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000057 DASRYE010000000
VERSION     DASRYE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 17033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17033
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197667"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="VFF98_03085"
     CDS             complement(<1..2363)
                     /locus_tag="VFF98_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62643.1"
                     /translation="MPAWAEGAAAGGADQAGDNAAAAKDTAEIIVTAQKRPEKLQEIP
                     VAAAVLSSDTMAHAHVSDLSDINRVVPSVEIKGTFNGRVPYGIRGISTNANEGAIGLT
                     SGVSIQVDGVPVPADSFAVNTITDVSQLEVLKGPQATLGGRTASAGVINFVTYGPTST
                     PHFAFNAMQTDDGEHHVDARASGPITDTLSYSLAGYYQHTPYPVYNLTLGEKSHADSY
                     GVRGKLKWQITPDFDAQVMGHYARSTSRGENFVPIYFTPGAAFFAPPVGLNQSDMFPG
                     YNINYKNTQYASHTQMGSRYQDKDGALVLNWHLGGNTITSTTSYSHEDQWQTQDVFEG
                     NDQNWALEFENFLYDIGKIPSAMVQPFNNQQVAYGYVRQVTEELKIASDAARPVSYIA
                     GFFYSDMTVNQSEFRNWTLNPLAKTNISTTTNYAIYGRLTAKLAEKVSLVGGLRYNWD
                     NIGWNVSEYFDPAHGIWGDGGFATGGFAWNLHDSSTALVGDIALQYKPSRNLMAYASY
                     TRGYKPKAFNTVHDFSTTQDDPSSTDAKSAQPAAQEHIDSFEVGFKSSLLNRHLTFNL
                     AAFYTKYSGYQAQLFDNSQIISVLVLSNADARTAGLEADLTYVKGNTRAQFSAAYVDA
                     KFTRFTGANCYPAQTAAEGCIAVTDSSGNPVYNASGTAVSAQDLTGKSLPDSPKLKLN
                     ASIQQVVPMDRFNVLLGGNISYRTATLLQADQNPATRQPGFALVDLSLGFQTKDEKAS
                     LTFFVNNLTNHYYLTNAEDFFASATTGNYVIGQPARDSHRYFGGRLSV"
     gene            2640..4187
                     /locus_tag="VFF98_03090"
     CDS             2640..4187
                     /locus_tag="VFF98_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU62644.1"
                     /translation="MSSAPNPTAPASSATASSAPATREAAAMSVRHAAVWAMAGQYIS
                     FAIMFANSVIISRFFLTPAEIGLFSIALAASMVAAVLQDFGLSRYIAGLRVVDAEALS
                     RCASVAFLFSFVVAGIIAASAWPMALTYHQKALFPILLIIAASYLFVPLSVVPMALMG
                     RTMSFHGHFCVNVGGAVALAGSALTLSALGLSSFALAWATLIAAIARAAIAQALRPAA
                     PWPLRTDGVRPIMANGAKLTTLYGIGALGSRTPDMILGKILGLVSVGLYSRAAGLSDQ
                     FRMLISGAIGQVFYPAFARIRDAGEPLGPAYLRVCAGYSAIIWPSMAGLALAAGPVVR
                     LLYGPRWEAAAPLLSLISLTEVLLISLPLVSDLPILLGRANRLIALNLVDTAMSVSLM
                     IAGCHWGVQGAAASRLLYGAASLVLYGRFMQGLVGFGVGRLLAIYARSAAAALAAVVP
                     LAATYRFWVSAADISLLQLLLASGAGVLCWLATLALLRHPALDDLLGIARHMAGAVLP
                     RLRVRAS"
     gene            4184..5023
                     /locus_tag="VFF98_03095"
     CDS             4184..5023
                     /locus_tag="VFF98_03095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZU62645.1"
                     /translation="MSIVIAILAHNEERRIAACLASLPLGAPGVEVHVLVNASQDATA
                     AIARQTAPAAHVHDWPEGGKARTWNRFVHEVAPPDGDAYVFVDGDAEVLAGSVEALAA
                     ALAARPPANAAAAFPRNGRHAARYAAEMAASHGLFGDLYALSGAFLRRLRASQLRLPD
                     DLIGDDSLIGALAKLDLGAAAWEDARILPCPAAGFLCEPVRATSPASLALQYRRMRAY
                     ALRRFQNRIISDIMATTGPAGLPRRMASLYRQWLPRLVPRRHPVWWWFDRQALALMRK
                     RAG"
     gene            complement(5035..5232)
                     /locus_tag="VFF98_03100"
     CDS             complement(5035..5232)
                     /locus_tag="VFF98_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62646.1"
                     /translation="MAWRGEPRKVPLFIELGLATGYPGHAVEPYAMAGLRMGPVRLGV
                     VPHVPGLNRTVVVHAMVQLRL"
     gene            5547..7037
                     /locus_tag="VFF98_03105"
     CDS             5547..7037
                     /locus_tag="VFF98_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62647.1"
                     /translation="MQGIVKPAAPAYVSDMPSSTCQSAPPRPGDAPAPPPPAIHLRWV
                     MFLVTAAMFMEILDGSIIVTALPAMARSFGTAPIALDLGISAYMLAVGVFIPVSGWVA
                     DRCGARAVLAGAIALFTLSSVLCGSANSLSAFVALRILQGVAGALMVPVGRLVIMHHT
                     PKDQLMAAMSSLVWPALIAPVLGPPLGGLITLHLGWRWIFYLNLPLGLLAFAATLWLI
                     PPTREEARRPFDWPGFLLCGGATFALLLGLDRISTGADGWALGLIGAGLVAGWATLRH
                     FRRAAAPMIGLGPWAIRTFRMSQRGGSLFRMAIGAAPFLLPLMFQIGFGYDAFHAGLL
                     VLAVFAGNLSTKTVTTQILRRFGYRPVLAVNGTLCAATLGACALLSPATPTWVVVAIL
                     FASGCTRSMQFTAINTVAFADVPRAQMADANGLSNTLGQLTMAAGITLGAMSVHGGGA
                     LAARLGWQAQGADYRIAFLAIAAVALAAVIDALRLPRGAADHFISR"
     gene            complement(7104..8642)
                     /locus_tag="VFF98_03110"
     CDS             complement(7104..8642)
                     /locus_tag="VFF98_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZU62648.1"
                     /translation="MFRDGSNWGRVACALAGTVSALALGAAANAQSVAPDSPNKNETG
                     GPLSSSMPPGASPAKPQPRQGEALPGGQTAPAGLPQAIRWTEDWSKPVAKDAPLMDKI
                     RHIPLGGNDDYYLSLGGEERIYYTDWNHAALGRTKNDSNSPVQSRARLIADLHLSNLF
                     RAYVELGDNREWGNSTATKPNQDQLDVYQAFVDVTVPMGKLGQITVRPGRYEMPLGNG
                     KLMGVREGLNMRFTYQGVRATYILPGQISVDFFTVRPVNIKDGAPFDDGPNHQQHYKG
                     VYVSSAKGLAGLGLGTDLYWYRVDRASATLREGTGVDERDNWGVRFWRRKKGIDFDLE
                     LNKQTGTFAGKTIDAWAVLFEGGYTFDNMPLKPRLGARFNAFSGDGNISDNKAGTFVP
                     ASARLPLISEAAFFNLSNLVDFYPSVTVRPAKNVSVMVGPDFLWRESRADGVYIGSTG
                     ASFAPYGSTHRIGTDLNLEASYQMTKRLSFRLFETYFDPGQAFKAAGGSHGNYFGFLT
                     DLRF"
     gene            complement(8727..10040)
                     /locus_tag="VFF98_03115"
     CDS             complement(8727..10040)
                     /locus_tag="VFF98_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62649.1"
                     /translation="MPMEDPRAIIAREPMHFRQILAIAIATALNALDGFDVLSISFAA
                     PGIAKEWHVTKQALGIVLSMELIGMGVGAFLLGALADRIGRRPTILGCLVVMASGMAL
                     ASSAHDVTTLSAFRLYTGLGIGGMLAATNAIVAECSNAQRRNLAVAIMVGGYPIGAVV
                     GGFIASSLLAATGHWQSVFQFGAGMTVVFIPLVLWLVPETIAFLLHKQPAGALEKINR
                     SMLRQGRPVLAALPPREEDKPGIALAQLFAPGVAGVTVLLCLAYFSHMLTFYFLMKWI
                     PKIVVDMGYAPPMAGSVLVWANVGGATGSVTLSLLTQRFSIRKLLIVLMVGGAVAVTA
                     FGQGLGTLAALSLVACIGGFFTNGATAGLYAVIAQSYPASLRGGGTGLVIGIGRAGAA
                     LGPILAGTLFEAHWPLSEVAAIMGCGTLIGGIAIAMLRYRESEIA"
     gene            complement(10070..11329)
                     /locus_tag="VFF98_03120"
     CDS             complement(10070..11329)
                     /locus_tag="VFF98_03120"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HZU62650.1"
                     /translation="MAQIIGAIASSHTPTIGFAQDAGKHEDPAWKPIFDGYAPVRAWL
                     EEKRPDVLFLIYNDHITSFFFDHYSTFALGVDEAYRPADEGGGARAVPAIAGDAQLSR
                     HIAQSLVTDGFDMSFFRGKGLDHGAFSPLSVLMDYKGGAWPTRIVPLQVGVLQFPIPT
                     ALRCWQLGEALRRAIESYPEDIRVAIVATGGLSHQVHGERCGFNNVGWDEAFLDLLEK
                     DPVALTRMTHAEYAELGGLEGAEVIMWLIMRGALSANVRRVHRSYYLPSMTGIATAIF
                     ENEARPMTAREADAHRTRMGAQLAGAEALAGTYPMTLERSARGFRLNTYLHSLIQPGP
                     RAAFKADEAAALAAADLSDEERQLIATRDWQGLIRYGASFFLMEKLAPIVGQGNLDIY
                     AAMRGESLEDFQKTRNQQINYSVAGQD"
     gene            11522..12478
                     /locus_tag="VFF98_03125"
     CDS             11522..12478
                     /locus_tag="VFF98_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62651.1"
                     /translation="MTDNHIEQDFSPETARPGSHSAVPVPRYILYGEAGTRPDWFVNV
                     EPLDKRCRTRGWVIKPHTHPRFSQIMLVTRGAGMMRVEAEDQPFGPGSVMVVPPHRIH
                     GFHYEQHTRGWVITIENHYFDDLLERAPALRQLMGRFGVTDLPVTVLSGLDGDFQRLM
                     DELDSQQSGSAIGAEIHLLAILLQLYRHWPQSVQAPPPGHSRGELVARFRQLVEAEFR
                     RQPPLPDMAAQLGVSVSQLRAACAQVAGLSPLAILHERLLGEARRCLAYTAQPITAIA
                     ADLGFSEASYFTRFFTRKTGDTPTAWRRGHSFLTGLSERPAA"
     gene            complement(12512..13138)
                     /locus_tag="VFF98_03130"
     CDS             complement(12512..13138)
                     /locus_tag="VFF98_03130"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZU62652.1"
                     /translation="MADARASLMGLWLAGQHGLAGLQAFMAANWLAFGAGQVLVAASG
                     VLPASMIALMAGATYGLGWGLLLSAISTMVGGWLAFALCRTALRERIARYVGHHPRVA
                     RLDEAMTSEGWRLVLLLRVSPVMPFALTSYGLGLTRISSRDFLLGTLASLPALTGYVA
                     LGALGRQGMAVAQGGASIWHWLMLAGGLAVVLYALARVRAALGRVGAA"
     gene            complement(13143..13934)
                     /locus_tag="VFF98_03135"
     CDS             complement(13143..13934)
                     /locus_tag="VFF98_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU62653.1"
                     /translation="MIDGKRVAVVLPAYNAGLTLLRTYREIPMDIVDDVILTDDASKD
                     DTSEIARGLGIHTIVHAKNRGYGGNQKTCYTAALERGADIVVMLHPDYQYSPRLVAAM
                     ASMIASGHYDVVLASRILGKGALAGGMPLYKYIANRALTFAENVLLGQKLSEYHTGYR
                     AWSRQVLETLPLEACSDDFVFDNQMLAQTAWFGFNTGEISCPTRYFEEASSINFRRSC
                     IYGLGVLKTALVYRLARWGLVRPALFHDLSARLKGVGAPAEAAAR"
     gene            14145..15368
                     /locus_tag="VFF98_03140"
     CDS             14145..15368
                     /locus_tag="VFF98_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62654.1"
                     /translation="MSAFYISGPLAFYYHFVRLWGIPPFYHPFDDTDTVMSALRCLRQ
                     GVDVIAANPCDPEARPFDYSPLWLALAVLPVTPAWLNGAGLLVDFGFFASLLLLPVAR
                     DGRAALALALAAVSSCAVLAVERGNNDLVLFVLAALAAVALGRAWGVRIIGYGLLLLA
                     GLLKYYPMAAMAVALREKVGRFVFLTLASLAITGLFVAITWHDLSRALAIIPEGTPFG
                     NMFGAVNLGAGIALDGHLPPVWPYAIRLALTGFAVFWATKRAGEPATRAALATLSARE
                     THFLMVGALLVVAAFFTAQNIGYRVIHLLLVLPALLALRHAGPAARFRHAPKVALLLM
                     WSELWRNRARWLGWHLGGKIGLGVGKYLAFAAKEAMWWWLVIVLASYVLAWLSQGPLC
                     QMAHRLLGGKAAAEG"
     gene            15472..>17033
                     /locus_tag="VFF98_03145"
     CDS             15472..>17033
                     /locus_tag="VFF98_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62655.1"
                     /translation="MKFPALALALPLALLGAGVAGADPAPFDLAGPALRISVSRDGTS
                     LPIAAVPQLAPGDRLTVEAPPPADATARYLLVAAFLRDPTNPPPAGWFARSESWKPAR
                     RGGGPIALTVPAGAHHLVLLLAPATGGDFATLKKAVAARPGAFVRAAQDLEQASLDRR
                     RYDAYLAAIRKISASTPDALARLAPVVASSLHIKINQDCLQRQAELQAACLLDAKQSS
                     VMGGDSTAGTSALSGAATDLALSLSATPAGGLGYYSPYISAIHEILGIFGAMHSAKYQ
                     YIPALGVPDGEALQLALNTPPSFGDPKSVLVAALPEIKPAQPPVPQLQPATPPAPALA
                     CLGGADPLPLALSPLFYATAYGHDLKLALHLPGGQRTELPLSADAARAGLVLAEPARL
                     PAGLSGTISASLHGMWGFTRFAGPEMTLQTAGAWAWQQPEARKAEAGPLLLSGAPAAC
                     VSAITAAGADGIARPVSWKATESGALALTLPASPDRPEPLALTITGPAGTPPARLVVA
                     PPARPAPPAARI"
BASE COUNT         2607 a         5797 c         5639 g         2990 t
ORIGIN      
        1 accgacaggc ggccgccgaa atagcggtgc gaatcgcgtg ccggctggcc gatcacgtaa
       61 ttgccggtgg tggcgctggc gaagaagtcc tccgcattgg tgaggtagta gtggttggtg
      121 aggttgttga cgaagaaggt caggctggcc ttctcgtcct tcgtctggaa gcccagcgac
      181 aggtccacca gggcaaagcc gggctggcgg gtggccgggt tctggtcggc ctgcagcagg
      241 gtggcggtgc gataggagat gttgccgccc agcagcacgt tgaaccggtc catcggcacc
      301 acctgctgga tgctggcgtt cagcttcagc ttggggctgt cgggcaggct cttgccggtc
      361 agatcctgcg cgctcaccgc agtgcccgag gcattgtaca ccggattgcc gctgctgtcg
      421 gtcacggcaa tgcagccttc ggcggcggtc tgcgcagggt agcagttggc gccggtgaag
      481 cgggtgaact tggcatcgac ataggcggcc gagaactgcg cccgggtgtt gcccttcaca
      541 taggtgagat cggcttccag gccggccgtg cgggcatcgg cattcgacag gaccagcacc
      601 gagatgatct gcgaattgtc gaacagctgc gcctgatagc cgctatactt ggtgtagaag
      661 gcggccaggt tgaaggtcag gtggcggttc agcaggctcg acttgaagcc cacctcgaag
      721 ctgtcgatat gttcctgggc cgcaggctgc gccgatttgg catcggtgct gctggggtcg
      781 tcctgggtgg tggaaaaatc gtgtaccgtg ttgaaggcct tgggcttgta gccacgggtg
      841 tagctggcat aggccatcag gttgcggctg ggcttgtatt gcagggcgat atcgcccacc
      901 agcgcggtcg aactgtcgtg caggttccag gcaaagccgc cggtggcaaa gccgccatcg
      961 ccccagatgc catgggccgg atcgaaatat tcgctgacat tccagccgat gttatcccag
     1021 ttgtagcgca ggccgcccac caggctcacc ttttcggcca gcttggccgt caggcggcca
     1081 tagatggcat agttggtggt ggtggagata ttcgtcttgg ccagcgggtt cagcgtccag
     1141 ttgcggaact cgctctggtt cacggtcatg tcggagtaga agaacccggc gatatagctg
     1201 accggccgcg cggcatccga ggcaatcttc agctcttctg tcacctgccg cacatagcca
     1261 taggccacct gctggttgtt gaagggctgc accatcgccg aggggatctt gccgatgtca
     1321 tagaggaagt tctcgaattc cagcgcccag ttctggtcgt tgccctcgaa cacatcctgc
     1381 gtctgccact ggtcctcgtg cgaataggag gtggtggagg tgatggtgtt gccgcccagg
     1441 tgccagttca gcaccagcgc gccatccttg tcctgatagc gcgagcccat ctgggtgtgc
     1501 gaggcatatt gggtgttctt gtagttgata ttgtagccgg ggaacatgtc cgactggttg
     1561 aggcccaccg gcggcgcgaa gaaggcagcg cccggcgtga aatagatcgg cacgaaattc
     1621 tcgccacgcg aggtggagcg ggcataatgg cccatcacct gcgcatcgaa atcgggcgtg
     1681 atctgccact tcagcttgcc gcgcacgcca tagctgtcgg cgtggctctt ctcgcccagc
     1741 gtgaggttat acaccggata gggcgtgtgc tgataatagc ccgcgaggct gtagctcagc
     1801 gtgtcggtga tcggcccgct ggcgcgcgca tccacatggt gctcgccatc gtcggtctgc
     1861 atcgcgttga aggcgaaatg cggcgtgctg gtgggcccat aggtgacaaa gttgatcacg
     1921 cccgccgagg ccgtgcgccc gcccagcgtt gcctgcggcc ccttcagcac ttccagctgc
     1981 gagacatcgg tgatggtgtt gacggcaaag ctgtcggccg gcaccggcac gccatccacc
     2041 tggatcgaca cgcccgaggt gaggccgatg gcgccctcat tggcattggt ggaaatgccg
     2101 cggatgccat agggcacgcg gccgttgaac gtgcccttga tttccaccga cggcaccacg
     2161 cggttgatat ccgacagatc ggacacatgc gcatgcgcca tggtgtcgct gctgagcaca
     2221 gcggcggcaa ccggaatttc ctgaagcttt tcggggcgct tctgcgccgt cacgatgatt
     2281 tccgcggtat ccttggcggc cgcggcatta tcgccggcct gatccgctcc ccctgctgcc
     2341 gccccttcgg cccaggctgg catcgccagc gccagaccca tcgcagatac agcaatcccc
     2401 cacgcaaact gcgcgccctt acgcatgata ttatcctccc ctcgatcctt cgagcttatg
     2461 gcggcaatgc taatcaggca gcggcggcgc gtctatacgg cgaaatcgcg atagtttgga
     2521 tgggcatata ttcacaaagc cgatgcgtgg cagccacgct atcgatggtt tcgctggctg
     2581 cgcgcaggcg gccagggcgg aacattttgt tcattccgcc ctgctagcaa gggcgggcca
     2641 tgtcctctgc ccccaacccc accgcgcccg ccagcagcgc caccgccagc agcgcccctg
     2701 ccacccgcga ggccgccgcg atgagcgtgc gccatgccgc cgtgtgggcg atggcggggc
     2761 agtatatctc ctttgccatc atgttcgcca attcggtgat catctcgcgc tttttcctca
     2821 caccggcgga aatcggcctg ttttccatcg cgctggcggc ctcgatggtg gccgcggtgc
     2881 tgcaggattt cggcctgtcg cgctatatcg ccgggctcag ggtggtggat gcagaggccc
     2941 tgtcgcgctg cgccagcgtg gcgttcctgt tctctttcgt ggtggccggc atcatcgccg
     3001 ccagcgcctg gccaatggcg ctcacctatc accagaaggc gctgttcccg atcctgctga
     3061 tcattgccgc cagctatctg ttcgtgccgc tatcggtggt gcccatggcg ctgatgggcc
     3121 gcacgatgag cttccatggc catttctgcg tcaatgtggg cggcgctgtg gcgctggcgg
     3181 gcagcgcgct cacgctgtcg gcgctggggc tgtcgtcttt cgcgctggcc tgggccacgc
     3241 ttatcgcggc catcgcgcgg gcggccattg cccaggcgct gcgcccggcc gcgccctggc
     3301 cgctgcgcac cgatggcgtg cgcccgatca tggccaatgg cgccaagctg accacgctgt
     3361 atggcattgg cgcgctgggc agccgcacgc ccgacatgat cctcggcaag atcctcggcc
     3421 tggtctcggt cgggctctac agccgcgcgg ccggcctgtc agaccagttc cgcatgctga
     3481 tctcgggcgc catcggccag gtgttctacc ccgccttcgc ccgcatccgc gatgcgggcg
     3541 agccgctggg cccggcctat ctgcgcgtgt gcgcgggcta ttcggcgatc atctggccca
     3601 gcatggccgg gctggcgctg gcggcgggcc cggtggtgcg cctactgtat ggcccgcgct
     3661 gggaagcagc cgcccccctc ctctcgctga tctcgctgac cgaggtgctg ctgatctcgc
     3721 tgccgctggt ctcggatctg ccgatcctgc tgggccgggc caaccggctg atcgccctca
     3781 accttgtcga cacggcgatg tcggtctcgc tgatgatcgc cggctgccac tggggggtgc
     3841 aaggggcggc cgcctcgcgc ctcctctatg gcgcggcctc gctagtgctg tatggccgct
     3901 tcatgcaggg gctggtgggc tttggcgtgg gccggctgct ggcgatctat gcccgcagcg
     3961 cggcggccgc ccttgccgcc gtggtgccgc tggcggccac ctatcgcttc tgggtgagcg
     4021 cggccgacat cagcttgctg cagctgctgc tggcctcggg cgcgggggtg ttgtgctggc
     4081 tggccacgct ggcgctgctg cgccacccgg cgctggatga tctgctgggc attgcccgcc
     4141 acatggccgg cgccgtgctg ccgcgcctgc gggtgcgcgc ctcatgagca tcgtcatcgc
     4201 catcctcgcc cataatgagg aacgccgcat cgccgcgtgc ctggccagcc tgccgctggg
     4261 cgcaccggga gtggaagtgc atgtgctggt gaacgccagc caggatgcca ccgccgccat
     4321 cgcccgccaa accgcccctg ccgcccatgt gcatgattgg cccgagggcg gcaaggcgcg
     4381 cacctggaac cgcttcgtgc atgaagtggc gccgcccgat ggcgatgcct atgtctttgt
     4441 cgatggcgat gccgaggtgc tggccggatc ggtggaagcg ctggctgcgg cgctggccgc
     4501 ccggccgccg gccaatgccg ccgccgcctt cccgcgcaat ggccgccatg ccgcccgcta
     4561 tgccgccgaa atggccgcca gccacgggct gttcggggat ctctatgcgc tttcgggcgc
     4621 tttcctgcgg cgcctgcgcg ccagccagct gcgcctgccc gacgacctca tcggtgacga
     4681 cagcctgatc ggcgcccttg ccaagctgga cctgggcgcg gccgcctggg aggatgcccg
     4741 catcctgccc tgccctgccg ccggcttcct gtgcgagccg gtgcgcgcca cctcgcccgc
     4801 cagccttgcc ctgcaatacc ggcgcatgcg cgcctatgcc ctgcgccgct tccagaaccg
     4861 catcatctcc gacatcatgg ccaccaccgg ccctgccggc ctgccgcggc gcatggccag
     4921 cctctatcgc cagtggctgc cgcgcctggt gccccgccgg cacccggtgt ggtggtggtt
     4981 tgatcggcag gcgctggcat tgatgcgcaa gcgggcgggc tgaaccggcc gccgctagag
     5041 ccgcagctgc accatcgcat gcaccaccac cgtgcggttc agccccggca catggggcac
     5101 cacgcccagc cggaccggcc ccatgcgcag gcccgccatc gcgtaaggct ccaccgcatg
     5161 gccgggatag cccgtggcca gccccagctc gatgaacagc ggcactttgc gcggctcgcc
     5221 ccgccaagcc acctgcgccg aagcccggcg atagctgtta taatagaccc cgcccagaaa
     5281 accgcagcgc ccctcaacat aaaggcctgg attaaagtca ttatagcccg cgcctgtatg
     5341 aacggacgca agatgaaggc caatggcggc gagatcgaaa cagtgcatgc cggttctttt
     5401 tgaaaaggag aaacagcctg cccctaaatc gcccgccgat aattttggaa atacgcggcc
     5461 acaccggcat atacctatgc ccgcatcggc gcggcgcggg cgcccctctc gccttgcaag
     5521 aggcccctgt tacccgcaag ggcctcttgc aagggatagt caagcccgcc gcgccggcct
     5581 atgtctctga tatgccttcc tccacctgcc aatccgcgcc gccccgcccc ggcgatgcgc
     5641 ccgccccgcc gccgcctgcc atccatctgc gctgggtgat gtttctcgtc accgccgcga
     5701 tgttcatgga aatcctcgat ggctcgatca tcgtcaccgc gctgccggcc atggcccgca
     5761 gctttggcac agcgcccatc gcgctcgatc tgggcatcag cgcctatatg ctggccgttg
     5821 gcgtgttcat cccggtcagc ggctgggtgg cggatcgctg cggggcgcgg gcggtgctgg
     5881 ccggcgccat cgccctgttc acgctctcct cggtgctgtg cggcagcgcc aacagccttt
     5941 cggcctttgt ggcgctgcgc atcctgcagg gggtggcggg cgcgctgatg gtgccggtgg
     6001 gccgccttgt catcatgcat cacacgccca aggaccagct gatggcggcg atgtcctcgc
     6061 tcgtctggcc ggcgttgatc gcgccggtgc tggggccgcc gctggggggg ctcatcacgc
     6121 tacatctggg ctggcgctgg atcttctatc tcaacctgcc gctgggcctg ctggcctttg
     6181 ccgccacctt gtggctgatc ccgcccacgc gtgaggaagc gcgccgcccg ttcgactggc
     6241 ccggctttct gctgtgcggc ggcgccactt tcgcgctgct gctggggctc gacaggatca
     6301 gcaccggcgc cgatggctgg gcgctggggc tgatcggcgc ggggctggtg gcaggctggg
     6361 ccacgctgcg ccatttccgc agggcagcag cgccgatgat tggccttggg ccctgggcca
     6421 tccgcacctt ccgcatgtcg cagcggggcg gctcgctgtt tcgcatggcg attggcgcgg
     6481 cgcccttcct gctgccgctg atgttccaga tcggctttgg ctatgatgcc ttccatgccg
     6541 gcctgctggt gctggcggtg tttgccggca acctctccac caaaaccgtg acgacgcaga
     6601 tcctgcgccg ctttggctat cgcccggtgc tggcggtgaa cggcaccttg tgcgcggcaa
     6661 cgctgggggc ctgcgcgctt ctgtcgcccg ccacgcccac ctgggtggta gtggcgatcc
     6721 tgtttgccag cggctgcacg cgttcgatgc agttcaccgc gatcaacacc gtggcctttg
     6781 ccgatgtgcc ccgcgcgcag atggccgatg ccaatggcct atcgaacacg ctgggccagt
     6841 tgaccatggc cgccggcatc acccttggcg ccatgagcgt gcatggtggc ggggcgctgg
     6901 ccgcccggct tggctggcag gcgcaagggg cggattatcg catcgccttc ctcgccattg
     6961 ccgccgtggc gctggctgcg gtgatcgatg cgctgcgcct gccgcgcggc gctgccgatc
     7021 atttcatcag ccgctaaaac gcgaagcggg cgggaaggct gctgcccccc gcccgcctct
     7081 atctgcggcc ctgccggcag cgctcagaag cgcaggtcgg tgaggaagcc gaagtagttg
     7141 ccgtggctgc cgccggccgc cttgaaggcc tggcccggat cgaaataggt ttcgaacagg
     7201 cggaacgaca ggcgcttggt catctggtag ctggcctcga gattgaggtc ggtgccgatg
     7261 cggtgggtgc tgccataggg cgcgaaggaa gcgccggtgg agccgatata gacgccatcg
     7321 gcccggcttt cacgccacag gaagtctggc cccaccatca cgctgacatt cttggccggg
     7381 cgcaccgtca ccgaggggta gaaatcgacg aggttcgaca ggttgaagaa ggccgcctcg
     7441 ctgatcagcg gcaggcgggc cgaagcgggc acgaaagtgc cggccttgtt gtcggagatg
     7501 ttgccatcgc ccgagaaggc gttgaagcgg gcgcccaggc gcggcttcag cggcatgttg
     7561 tcgaaggtgt agccgccctc gaacagcacc gcccaggcat cgatcgtctt gccggcgaaa
     7621 gtgccggtct gcttgttcag ctccaggtcg aagtcgatgc ccttcttgcg gcgccagaag
     7681 cgcacgcccc agttgtcgcg ctcgtcaacg ccggtgccct cgcgcagggt ggcgctggcg
     7741 cggtccacgc gataccagta aaggtcggtg ccaaggccca gccccgccag ccccttggcc
     7801 gagctgacat aaacgccctt gtagtgctgc tggtggttgg ggccatcatc aaagggggcg
     7861 ccatccttga tgttcaccgg gcgcacggtg aagaaatcca cgctgatctg gccgggcagg
     7921 atataggtgg cgcgcacacc ctgataggtg aagcgcatgt tcagcccttc gcgcacgccc
     7981 atcagcttgc cattgcccag cggcatttca tagcggcccg ggcgcacggt gatctggccc
     8041 agcttgccca tcggcaccgt cacatcgacg aaggcctgat agacatcgag ctggtcctgg
     8101 ttgggcttgg tggcggtgct gttgccccat tcgcggttgt cgcccagttc cacataggcg
     8161 cggaagaggt tgctgaggtg caggtcggcg atcaggcgcg cgcggctttg caccgggctg
     8221 ttgctgtcgt tcttggtgcg gcccagcgcg gcatggttcc agtcggtgta gtagatgcgc
     8281 tcctcgccgc ccagcgagag gtagtagtca tcattgccgc ccagcgggat gtggcggatc
     8341 ttgtccatca gcggggcatc cttggccacc ggcttcgacc agtcttccgt ccagcggatg
     8401 gcctgcggca ggccggccgg cgccgtctgc ccgccgggca gcgcctcgcc ctgcctgggc
     8461 tgcggcttgg ccggcgatgc gcccggcggc atcgaggaac tcagcgggcc gccggtctcg
     8521 ttcttgttcg gcgaatcggg tgccaccgat tgcgcattgg ccgcagcgcc cagcgccagt
     8581 gccgataccg tgcccgccag tgcgcaggcc acacgtcccc aattgctccc atcacggaac
     8641 attgcatacc tcccagtaat tgcttgtgaa aaatttgtgt attggggtgg ccggcgatcc
     8701 tgccggccac acccggatcg tttaattcag gcgatctcgc tttcgcgata gcgcagcatg
     8761 gcaatggcga tgccgccgat cagcgtgccg cagcccatga tcgcggccac ttcgctcagc
     8821 ggccaatgcg cctcgaacag cgtgcccgcc aggatcggcc ccagcgccgc gcccgcccgg
     8881 ccaatgccga tgacaaggcc cgtgccgccg ccgcgcagcg atgccggata ggattgcgcg
     8941 atcaccgcat aaaggccggc cgtggcgcca ttggtgaaga agccgccaat gcaggccacc
     9001 agcgacagcg cggccagcgt gcccagcccc tggccaaagg cagtcaccgc cacagcgccg
     9061 cccaccatca gcacgatcag cagcttgcgg atgctgaagc gctgggtcag caggctgagc
     9121 gtgaccgagc cggtggcccc gccgacattg gcccacacca gcacgctgcc tgccatcggc
     9181 ggcgcatagc ccatgtcgac gacgatcttg gggatccact tcatcaggaa gtagaaggtg
     9241 agcatgtggc tgaaataggc caggcacagc agcaccgtca cgccggcaac gccgggggca
     9301 aacagctggg ccagggcaat gccgggcttg tcctcttccc tgggcggcag ggcggccagc
     9361 acggggcggc cctggcgcag catcgagcgg ttgatcttct ccagcgcgcc ggccggctgc
     9421 ttgtgcagca ggaaggcgat ggtctcgggc accagccaca gcaccagcgg gatgaacacc
     9481 accgtcatgc cggcgccaaa ctggaacacc gattgccagt ggccggtggc cgccagcagg
     9541 ctggaagcga tgaagccgcc caccaccgcg ccgatcggat agccgcccac catgatcgcc
     9601 acggccaggt tgcgccgctg ggcgttggaa cattccgcca cgatggcgtt ggtggcggcc
     9661 agcatgccgc caatgcccag cccggtgtac agccggaagg ccgagagggt ggtcacatca
     9721 tgcgcggagg acgccagcgc catgccgctg gccatcacca ccaggcagcc cagaatggtg
     9781 ggccggcggc caatgcggtc ggccagcgcg cccagcagga aggcgcccac gcccatgccg
     9841 atcagttcca tcgacaggac aatgcccagc gcctgcttgg tgacatgcca ttccttggcg
     9901 atgccgggcg ccgcaaagct gatcgacaga acgtcgaaac cgtcgagcgc attgagcgca
     9961 gtggcgatgg caatcgccag tatctggcga aaatgcatcg gctcgcgcgc gatgatggcg
    10021 cgtgggtctt ccatgggcat cctctctctt gcgggccgca gggcggttat cagtcctgcc
    10081 cggccacgga ataattgatc tgctggttgc gggtcttctg aaagtcttcc aggctctcgc
    10141 cgcgcatggc ggcatagata tcgaggttgc cctggccgac gatcggcgcc agcttttcca
    10201 tcaggaagaa gctggcacca taccggatca gcccctgcca gtcacgcgtg gcgatcagct
    10261 ggcgctcctc atcggaaagg tcggccgcgg ccagggctgc ggcctcatcg gccttgaagg
    10321 cggcgcgcgg gcccggctgg atcaggctgt gcagataggt gttgaggcgg aaaccccggg
    10381 cagagcgttc cagcgtcatc ggataggtgc ccgccagtgc ctcggcgccg gccagctgcg
    10441 cgcccatgcg ggtgcggtgc gcgtcggcct cgcgcgcagt catcgggcgc gcctcattct
    10501 cgaagatggc ggtggcgatg ccggtcatgc tgggcaggta atagctgcga tgcaccctgc
    10561 gcacattggc cgacagcgcc ccgcgcatga tcagccacat gatcacctcg gcgccttcca
    10621 gcccgcccag ttcggcatat tcggcatggg tcatgcgggt gagcgcgacc gggtccttct
    10681 ccagcagatc gaggaatgcc tcgtcccagc cgacattgtt gaagccgcag cgctcgccat
    10741 gcacctggtg cgacaggcca ccggtggcca cgattgccac cctgatgtcc tcggggtagc
    10801 tttcgatggc gcggcgcagc gcctcgccca gctgccagca gcgcagcgcg gtgggaatgg
    10861 gaaactgcag cacgcccacc tgcagcggca cgatccgcgt gggccaggcg ccgcccttgt
    10921 agtccatcag caccgacagc ggcgagaagg cgccatgatc cagccccttg ccgcggaaga
    10981 agctcatgtc gaaaccatct gtcaccagcg actgggcgat gtggcgcgac agctgcgcat
    11041 cgcctgcaat ggccggcacg gcgcgcgcgc cgccgccttc gtcggccggg cgataggcct
    11101 cgtccacgcc cagcgcgaag gtggaataat ggtcgaagaa gaaggaagtg atgtgatcgt
    11161 tgtagatcag gaacagcacg tcggggcgct tttcctccag ccaggcgcgc accggggcat
    11221 agccatcgaa gatcggcttc caggccggat cctcatgctt gccggcatcc tgggcgaagc
    11281 cgatggtggg ggtgtgcgaa ctggcgatgg cgccgatgat ctgagccatg atctctctct
    11341 tcagccgatg cggcggccac aggcgggcgc ggccgggcct tcgggcaggg gaatgggggc
    11401 aaattgcagg ggcatggccg ttcgtcttgc atcctcatca cggagcagtg tcttgccatg
    11461 gggccaaagc ccggcgcgcc tgccgattgc gcacccatgc ccgatgggat aggcaggcgc
    11521 catgacggat aatcacattg agcaggactt ttcgccagaa accgcccggc cgggcagcca
    11581 cagcgccgtg cccgtgcccc gctatatcct ctatggcgaa gccggcaccc ggcccgactg
    11641 gttcgtcaat gtcgagccgc tcgacaagcg ctgccgcacc cgcggctggg tgatcaagcc
    11701 ccacacccac ccccgcttca gccagatcat gctggtgacg cgcggcgccg gcatgatgcg
    11761 cgtggaagcc gaggaccagc cctttggccc gggcagcgtg atggtggtgc cgccgcaccg
    11821 gattcacggc ttccattatg aacagcacac gcgcggctgg gtgatcacga tcgagaatca
    11881 ctatttcgac gacctgctgg aacgcgcgcc ggcgctgcgc cagctgatgg gccgctttgg
    11941 cgtgaccgac ctgccggtga ccgtgctgag cgggctggat ggcgatttcc agcggctgat
    12001 ggacgagctg gacagccagc agagcggcag cgccatcggc gccgagatcc atttgctggc
    12061 aatcctgctg cagctttatc ggcactggcc gcaaagcgtg caggcgccgc cgccgggcca
    12121 cagccggggc gaactggtgg cgcggttccg ccagctggtg gaggccgaat tccgccgcca
    12181 gccgccgctg cccgatatgg cagcccagct cggggtcagc gtatcgcagt tgcgggcggc
    12241 ctgcgcgcaa gtggcgggcc tgtcgccgct ggcgatcctg cacgagcggc tgctgggcga
    12301 ggcacgccgc tgcctagcct ataccgccca gccgatcacc gccatcgccg ccgatctggg
    12361 cttttccgaa gcgtcctatt tcacccgctt cttcacccgc aagaccggcg atacccccac
    12421 cgcctggcgc cgcggccaca gcttcctcac cggcctcagc gaacggccgg cagcctagcc
    12481 caagggcctg cggcccagcc caacggcgcc ctcaggccgc gcccacccgc cccagcgccg
    12541 cgcgcacccg cgccagcgca tagagcacca ccgccagccc gccggccagc atcagccagt
    12601 gccagatgct ggccccgccc tgcgccaccg ccatgccctg ccggcccagc gcccccagcg
    12661 ccacatagcc ggtgagcgcg ggcagcgagg ccagcgtgcc cagcaggaaa tcgcggctgc
    12721 tgatccgcgt gaggcccagc ccatagctgg tgagcgcaaa gggcatcacc ggcgagacgc
    12781 gcagcagcag caccagccgc cagccctcgc tggtcatcgc ctcgtccagc cgggccacgc
    12841 gcggatgatg gcccacatag cgggcaatgc gttcgcgcag cgccgtgcgg cacagggcaa
    12901 aggccagcca gccgcccacc atcgtgctga tcgccgacag cagcagcccc cagcccagcc
    12961 cataggtggc ccccgccatc agcgcgatca tcgaggccgg cagcacgccg ctggccgcca
    13021 ccagcacctg gcccgcgcca aacgccagcc agttggccgc catgaaggcc tgcaggccgg
    13081 ccagcccatg ctgccccgcc agccacagcc ccatcagcga cgcgcgcgca tcagccatgc
    13141 aatcagcgcg ccgccgcttc ggccggcgcg cccacgccct tcagccgggc cgacagatca
    13201 tggaacagcg ccgggcgcac cagcccccag cgcgccagcc gatagaccag cgccgttttc
    13261 agcacgccca gcccatagat gcagctgcgg cggaaattga tgctcgaggc ttcctcgaaa
    13321 tagcgcgtgg ggcaggagat ctcgccggtg ttgaagccga accaggcggt ttgcgccagc
    13381 atctggttgt cgaacacgaa atcgtcggaa caggcttcca gcggcagggt ttccagcacc
    13441 tggcggctcc aggcgcgata gccggtgtga tattccgaca gcttctggcc cagcagcacg
    13501 ttttcggcaa aggtcagcgc gcggttggcg atgtatttgt agagcggcat gccgccggcc
    13561 agcgccccct tgcccagaat gcgcgaggcc aacaccacat cataatggcc agaggcgatc
    13621 atgctggcca tcgcggccac cagcctgggc gaatattgat aatccgggtg cagcatgacg
    13681 acgatatcgg cgccgcgttc cagggctgcg gtgtagcagg tcttctggtt gccgccatag
    13741 ccgcggttct tcgcatgaac gatggtgtgg atgccaaggc cgcgagcgat ctcgctggta
    13801 tcgtccttgc tggcatcatc ggtcaggatc acatcgtcga cgatgtccat cgggatctcc
    13861 cgataggtcc gcagcagggt cagccccgca ttataggccg gcagcacaac cgccacgcgc
    13921 ttaccatcaa tcatccagaa ggcctcgtca atcgggtcta ggcaacaagc ccgcttcctg
    13981 tattaacctc gattatcatg tcaatataat tggcggaact acttatggaa atatacgaag
    14041 aacatcccaa tgtaatccgc aaagattgcg caaaagatat gaaacacaaa aaccttctta
    14101 tgaaatattt cccatttgta gcatcaattt tgcttttttc attcatgtct gcattttata
    14161 tcagcgggcc tctggcgttt tattaccatt tcgtccggct gtggggcatt ccaccttttt
    14221 atcacccctt cgatgatacc gacacggtga tgtcggccct gcgctgcctg cgccaggggg
    14281 tggatgtgat cgccgccaac ccttgcgatc cggaggcgcg cccgttcgat tattcgccgc
    14341 tgtggctggc gctggcggtg ctgccggtta cgccggcctg gctcaacggg gccgggctgc
    14401 tggtggattt cggctttttc gccagcctgt tgctgctgcc tgtggcgcgc gatggccggg
    14461 ccgcgctggc gctggcgctg gcggcggttt ccagctgcgc ggtgctggcg gtggaacgcg
    14521 gcaacaatga tctggtgctg tttgtgctgg cagcgctggc ggcggtggcg ctgggccggg
    14581 cgtggggcgt gcgaatcatc ggctatgggc tgctgctgct ggcgggcctg ctcaaatatt
    14641 atccgatggc ggcaatggcg gtggccctgc gcgagaaggt cgggcggttt gtcttcctca
    14701 cgctggcctc gctggcgatc accggcctgt tcgtggcgat cacctggcac gatctgtcgc
    14761 gcgcgctggc gatcattccg gagggcacgc cctttggcaa catgttcggc gcggtgaacc
    14821 tgggcgcggg cattgcgctg gatggccatc tgccaccagt gtggccctat gccatccgcc
    14881 tggcgctcac cggctttgcc gtgttctggg ccaccaagcg cgctggcgag ccagccaccc
    14941 gcgcagcgct cgccacgctt tcggcccgcg agacgcattt tctgatggtc ggcgcgctgc
    15001 tggtggtggc cgccttcttc accgcgcaga acatcggcta ccgggtgatc cacctgctgc
    15061 tggtgctgcc cgcgctgctg gcgctgcgcc atgccgggcc cgcggcgcgc tttcgccatg
    15121 cccccaaggt ggcgctgctg ctgatgtgga gcgaattgtg gcgcaaccgg gcgcgctggc
    15181 tgggctggca tctgggcggc aagatcggcc ttggcgtggg caaatatctg gcctttgccg
    15241 ccaaggaagc gatgtggtgg tggctggtga tcgtgctggc ttcctatgtg ctggcctggc
    15301 tgtcgcaggg gccgctgtgc cagatggcgc accgcctgct gggcggcaag gcggcggccg
    15361 agggctgagc aggcgccatt ttgttcatgt cggatcaggg ggaaggcgtg taaggcggcc
    15421 gggcgggcgc cgcgggggtg gcccgatcag catagcggcg ccttttttgc gatgaaattt
    15481 cctgctcttg cccttgctct cccgctggcc ctgctgggcg ccggcgtggc cggggccgat
    15541 cccgccccgt tcgatctagc cggccctgcc ctgcgcatct cggtcagccg cgatggcacc
    15601 agcctgccga tcgcggccgt gccccagctg gcccccggcg acaggctgac cgtggaagcc
    15661 ccgccgcccg ccgatgccac cgcgcgctat ctgctggtgg ccgccttcct gcgcgatccc
    15721 accaatccgc cgccggccgg ctggttcgcc cgatcggaaa gctggaagcc ggccaggcgc
    15781 ggcggcgggc cgattgcgct caccgtgccg gcaggggccc accatctggt gctgctgctg
    15841 gcgccggcca ccggcggcga tttcgccacg ctgaagaagg cggtggccgc gcggcccggc
    15901 gcctttgtgc gcgccgcgca ggatctcgaa caggcctcgc tcgaccggcg gcgctatgat
    15961 gcctatctcg ccgccatccg caagatctcg gccagcacgc ccgatgcgct ggcgcggctg
    16021 gcgccggtgg tggccagcag cctgcacatc aagatcaacc aggattgcct gcagcgccag
    16081 gcagagctgc aggccgcctg cctgctggat gccaagcaat cctcggtgat gggcggcgac
    16141 agcacggcgg gcaccagcgc gctgtcgggc gcggccactg atctggcgct cagcctcagc
    16201 gcgacgcccg ccggcgggct gggctattac agcccctata tcagcgccat ccacgagatc
    16261 ctcggcattt tcggggcgat gcattcggcg aaataccagt atatcccggc gctgggcgtg
    16321 cccgatggcg aggcgctgca gctggcgctc aacacgccgc cctcctttgg cgatcccaaa
    16381 tcggtgctgg tggcggccct gcccgagatc aagccggccc agccgccggt gccccagctg
    16441 cagccggcaa cgccgcccgc ccccgccctc gcctgcctgg gcggggcaga tccgctgccg
    16501 ctggcgctca gcccgctgtt ctatgccacc gcctatgggc atgatctgaa gctggccttg
    16561 cacctgcccg gcggccagcg caccgaactg ccgctgagcg ccgatgccgc ccgcgccggg
    16621 ctggtgctgg cagaaccggc gcggctgccg gccgggcttt ccggcacgat cagcgccagc
    16681 ctgcatggta tgtggggctt cacccgcttt gccgggccgg agatgaccct gcagaccgcc
    16741 ggcgcctggg cctggcagca gcccgaggca cgcaaggccg aagccggccc gctgctgctg
    16801 agcggcgcgc cggcggcctg cgtcagcgcc atcaccgccg ccggggccga tggcatcgcc
    16861 cgcccggtca gctggaaagc caccgagagc ggcgccctcg ctctcaccct gccggccagc
    16921 cccgaccggc ccgagccgct ggcgctgacc atcaccggcc ctgccggcac gccgcccgcc
    16981 cggctggtgg tggccccgcc cgcccggccg gcgccgcccg ccgcccgcat cag
//
LOCUS       DASRYE010000058        15430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_215459,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000058 DASRYE010000000
VERSION     DASRYE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215459"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFF98_03150"
     CDS             complement(<1..792)
                     /locus_tag="VFF98_03150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62656.1"
                     /translation="MLLLGLVVLAVWLGLAATGFWTCAQRDTRTPGLLTPPLPAVVAV
                     VPARDEADVIARSIGSLLAQDYKGPFRIVLVDDNSSDGTGAIARALDGAERLTVLTGA
                     PLADGWTGKLWAVSQGIAAAGTPDYLWLTDADIEHTPDTLSTLVGIARSGDRRLVSFM
                     AKLHCARPAEKLLIPAFIWFFMMLYPFGWINRRGRFTRRVAGAAGGCVLVKRKTLEAA
                     GGIAAMRGALIDDCTLGALIKKHGPIWLGLTDRSRSIRPYESWREI"
     gene            complement(792..1787)
                     /gene="hpnA"
                     /locus_tag="VFF98_03155"
     CDS             complement(792..1787)
                     /gene="hpnA"
                     /locus_tag="VFF98_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZU62657.1"
                     /translation="MDGQTTIVTGVSGFLGSAVARQLAARGRRLVGLVRASSPRGNLA
                     DFPGELIEVDLRDEAGVARAMAGAGALYHVAADYRLWARDPEEILRNNLAMTRAVMEG
                     ALAAGVGRVVYTSSVATLLPGHGEPSDETRPASPEQAVGIYKRSKVVAERLVEELVAT
                     RGLPAVIVNPSTPIGPRDIKPTPTGRVLVEAANGKMPAYVESGLNLVHVDDVALGHIL
                     AAEKGQIGRRYVLGGEDVTLGQMLADICAMVGRKPPRVKLPRAPLFPLAYAAELGGRI
                     TGKEPFLTLDALRMAGHHMYYSSARARAELGYATRPYQEAIADALAWFRAAGMIG"
     gene            1935..3134
                     /gene="hpnI"
                     /locus_tag="VFF98_03160"
     CDS             1935..3134
                     /gene="hpnI"
                     /locus_tag="VFF98_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZU62658.1"
                     /translation="MVLSVILARAVAGLVVVLALVAIAYQLAALALLRRFFARAAGAA
                     APAHWPAVSLLKPLHGAEPRLGANLASFLAQDYPAPVQMVCGVNALGDPAVGALKALS
                     ARHPGAHISLSTGPRQPGANAKMGNCAAFYPLARHDVLVLSDSDMVVGRDYLARVVAQ
                     LLRPGVGAVSCLYVGRGDAGVWSRIGAAIISWQTMPNMVLGLALGMARPCMGSTIAIR
                     REVLEAIGGFGAFADILADDHAIGAAVHGCGLEVAIPPMLLVHAGAEGSARVLWRQHL
                     RWAATVRDLAGAGHYGSVVTHALALALLAAPCVAVAGLPAAWGAGLVAAALAVRLALA
                     GDVARRMKRLDGAEQLPFWLLPVADIFAFAVFLASWSARKIDWRGLPLTITGSGRIAH
                     SGAPSND"
     gene            3142..4587
                     /gene="hpnJ"
                     /locus_tag="VFF98_03165"
     CDS             3142..4587
                     /gene="hpnJ"
                     /locus_tag="VFF98_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HZU62659.1"
                     /translation="MKRTLFLQAPSFDGYDGGAGARYQMKREVKSFWYPTWIAQPAAM
                     VPGSKLIDAPSHDLSWEDIAHEFDERDLVILHTSTPSFNQDVRTAELIKARNPNIQIA
                     FVGAKVMIEDEKSLAASPAIDLVCREEYDFTCVDLAEGKPLAEIDGITWRAPDGSIVR
                     NKDRKAIEDMDVLPMVSPIYKRDLDSTKYFGGYQRHPYVSFYTGRGCKSRCTFCLWPQ
                     TISGHNYRYRSVPKVIEEVKYILANMPEVKEIFFDDDTLADAHEHVVALSRELRKLGF
                     GTKGFDKTWGCNAKANVPYDVLKEMKAGGCRVLLVGYESGNQKILHNIKKGLRTDVAR
                     QFTKDAHSLGLTIHGTFILGLPGETLETIEETIRYAIELNPHTIQVSLAAPYPGTFLY
                     KQATENGWFDGTDHLLTDGGNQIAQLSYPHLSSAVIFDKVEEFYRRYYFRPSKIASIV
                     GEMLRDWDMMKRRLREGVEFFDFLRRRKEAA"
     gene            4665..5504
                     /gene="hpnK"
                     /locus_tag="VFF98_03170"
     CDS             4665..5504
                     /gene="hpnK"
                     /locus_tag="VFF98_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HZU62660.1"
                     /translation="MTADDFGAALAVNEAVERAHVDGILTAASLMVAGEGAADAVARA
                     RRLPGLGVGLHVVLVEGRPVLPAAQVPALVGPDGQFAPNMVRTAFRIAFSRRARAQMR
                     AEVAAQFAAFAATGLPLDHVNAHKHFHLHPMIAAAILEAGRAHGLAAIRVPREGGGSG
                     LGALLMRWWAGLLGRRWRRAGLATNDQVVGLAVSGAFTPERMRAALASLPEGITEIYT
                     HPATADAYPGSAPGYAYRAELAALVDPVVCKLAGAVARGPFRALATLSCPSGSHSSAI
                     EAA"
     gene            5507..6628
                     /locus_tag="VFF98_03175"
     CDS             5507..6628
                     /locus_tag="VFF98_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZU62661.1"
                     /translation="MFATQLVGLGLMAAGVPPESALESANAVVPVAEAAPAAAPAPVV
                     APASAQPAPAQPAAADTTPIDDPETAAAIHHMACEAVATGDDDEGQLAADAGVDPAQA
                     VCPPPPPTDPDPLKGFNRLSFAISLPIDRYIVRPLAMVYKAVLPAFLRDGIHNALENI
                     YFPTILVNDMLQLRPDLTGKTLMRFFVNSTLGLGGLFDVAKRKPFNTPAHANSFGDTL
                     GLMGFEAGPYLYFPVMGPSSTRDLAGMIADAFTQPVLFDYVSHTHVKTVHQRERSFVT
                     STISLTTAGASALVARGLDERVRADADLKALKAQSVDFYTNMRLSYLQNREAEIAELK
                     TRGGKPADVPAFDEPLEDPGAVKAPQQPPQPQPNSQAKP"
     gene            complement(6632..7672)
                     /locus_tag="VFF98_03180"
     CDS             complement(6632..7672)
                     /locus_tag="VFF98_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZU62662.1"
                     /translation="MTQPASPAAPAPASRPRWHGWAVPAVILVAGLASVAANPGLRSL
                     FAQAARQIDPLAVLVTLPVQLVAILVCTAAQQALNIGVPFRASFLARLTRDAGHNLLI
                     FPPGMGEAIGARMLVLAGGKARAALMLRVLDVAAEVLAELPYMALAGWVVAGFWLGGH
                     GAAGLPSGGGASAAHGWLVAGGMVALVLLLVGLAARRWWRGLPQGHRLRRRIAAEWHL
                     LRREMHRQRTGLPLAIGLHVIGWGLSGVQVWLAAGVFGLHLSLFSALAIESAATSARV
                     ILFFVPGGLVMQEAGAVLAGAALGVPAPMALALSLVLRLRDVAFGAALLLWPAMEWRA
                     RRQQARPRAALA"
     gene            7821..10427
                     /locus_tag="VFF98_03185"
     CDS             7821..10427
                     /locus_tag="VFF98_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZU62663.1"
                     /translation="MYIARLNQAILAAARRPRLVLAGVLALAAAALAVVIGDFDMTTD
                     TGELISASTPWRQDESRVENAFPQLKDSVLIVVDGATPELAEDGASRLAAALADDKAH
                     IVDVKRPDGGPFFDREGLLFASLADVREATRRLIDAQPLLGGLASDPSLHGIAATLAT
                     VADGAAKPGAAPADQLATPLARLSSAIDDRLAGRHVFFSWQRLFADGAGPLSPPIRRV
                     LVVHPRLDFSDLEPGSAAVDAINQTAARLQIDAAHGQHVGITGQVPLADEEFGSIREN
                     IGLVGALMVAAMVVCLWLATRSARMVGAIMGTIAVGLVITLALGLLAVHRLNLISVAF
                     IPLFVGLGVDFGIQVSVRFNAERAAGAEPMQALERMAAAIGEPLLMAAGAIFLALAAF
                     LPTAYIGIAELGVIAGLGMIVAFGLNITLLPALLVLLKPAAPAHRVGWAGAAAADRWL
                     HRHRKGILASFAGAVVATIAALPLVAFDFNPLHLRDPRAPAMATLADLMRDADRTPNT
                     ISILAPDAGQAGALAARLGRLPEVAHAVSIDSFVPEGQPDKLALIQDASLLLDAAINP
                     FDLPTSADDTATQAKLHEAADRLRALAAARPGALGRSAAALAVSFERLAAASPASRAD
                     IAAMLEMPLAITLDKIRASLQAGEVSRASLPADLAADWVAKDGSALVQVTPAGNSLDN
                     RVLTRFTAAVRAQAPHASGLPVATQEAARTVAGAFVQAGVLALLLVSGLLWLVLRSVR
                     EVAFTLAPVVLSGFLTLGSCVLIGQPLNFANIIAFPLLFGVGVAFHIYFVMAWRRGVE
                     NLLQTSLARAVVMSALATGSAFGALWLSHHPGTASMGLILMISLIWTLVCALVFEPAL
                     LGPVHGPAREDV"
     gene            10427..11242
                     /locus_tag="VFF98_03190"
     CDS             10427..11242
                     /locus_tag="VFF98_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU62664.1"
                     /translation="MGAPPATDLAASDAADPRARLLLASIHDVGPRFEREVDQLAERL
                     DGHLGSARFAMLVVPDHWGAAPLLTAGGFQARLRGWADAGVEMFVHGWFHRDMAAHAG
                     LAAFKARHMTASEGEFLGLAQEEAARRMDEGRKLVEDIIGRPAAGFIAPAWLYGPGAR
                     AALATSGFALAEDHLRVWQPGTGRVLARGPVITWASRSKARQRSSRFFAGLARHALRG
                     QQVARIAVHPGDTTVPALLSSIDATIAAFTRAHRPGRYAELLPARPCQVQAAL"
     gene            11239..12255
                     /locus_tag="VFF98_03195"
     CDS             11239..12255
                     /locus_tag="VFF98_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62665.1"
                     /translation="MIERTTQPFGGDEASDRPARAESDMAQMYQHSFAAGEGSPETRL
                     DMGTVEQRQRGSRLAGLFSAAISLAMLVAVAMEIRQLHFREILAMIPRSAPFWLAFAA
                     YYLVPPFTEWTIYRRLWGLPLSGVAALLRKQVSNELLLGYLGEVQFYAWARARLNMVA
                     APFGAIKDVTILSALTGNIATLVMLVGAWPLVMSGQLGMESRTVFMSLGVVLLTSCII
                     LFFRQRLFSLHKRDLLIITGLHFGRIIIIVGLWALMWHLVLPEVELAMWLVLATLRML
                     ISRLPLVPNKDVVFAGVAVFLLGHEAQISHLIAMMAGLLPVTHIVVGAIFASVDLFQS
                     RKES"
     gene            12259..12825
                     /locus_tag="VFF98_03200"
     CDS             12259..12825
                     /locus_tag="VFF98_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HZU62666.1"
                     /translation="MRLSPALLAGLAATSVLAAGPAQAGDKPVVPLPSTPDLGKAEAH
                     CRAGEQGPALLISVVGLKDRAGRLKLEVYPSNDEDFLMDDNVLVYQHKAFRRVEEEVP
                     ASGAVQLCARIPGPGAYSVMLLHDRDSNRKFGWMIDGIGFSANPRLGWHKPKAAATRV
                     MAGTGLTRINIVLNYRHGLGMSPMRDGD"
     gene            12883..14037
                     /locus_tag="VFF98_03205"
     CDS             12883..14037
                     /locus_tag="VFF98_03205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62667.1"
                     /translation="MRIVDVCAFYSPQGGGVRTYVEQKLAIGPQLGHEIIVLAPGDRA
                     EVIDRGPRARIVTIPAPRFPLDRKYWYFNDEPALHAAISGLAPDFLEVSSPWRSAAMA
                     ARWPGTAPRALVMHADPLSAYAYRWLQPLFSRETIDRQFAPFWKHLREMGKSYDRVIC
                     ASRELKERLARGGVSHTSLHPMGIEAGVFSPERRSPALRRRLLALCGLPESARLLIGV
                     GRLSAEKRWPMVVDAVTVASQSMPIGLVLVGQGRQKYRIERQIFGNPHIQLLGVEKDR
                     QKFATLLASADALIHGCEAETFCMAAAEARASGVPVIVPDRGGAAEHARGGAGITYRS
                     GNAVSAAHAALRLLHKPPRVPFADVVTMEDHFAGLFAEYAALLAGRSAAA"
     gene            complement(14057..14506)
                     /locus_tag="VFF98_03210"
     CDS             complement(14057..14506)
                     /locus_tag="VFF98_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZU62668.1"
                     /translation="MPPGGWANLIAEFAVSSLMVVFCVCVHGMGLFGLTRALRSEASS
                     ERLQHIRPLSPRGVAFTLAIVISIIALHGVEIWAFALVYVATGAVRGFESALYFSTIS
                     YSTVGYNDAHITLAWRLLGAFESILGVILLGWSTSFFFRMLSRIDAH"
     gene            complement(14533..>15430)
                     /locus_tag="VFF98_03215"
     CDS             complement(14533..>15430)
                     /locus_tag="VFF98_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HZU62669.1"
                     /translation="GGRPDPRQDRHQRTAARLMRRRAPALALWPALLGAALAMASAAP
                     ARLPAQSAPHAPASAAPVVPAREIRRLPHDPAAFTEGLFIDRGELWESTGMEGRSSIR
                     QVDLESGRVIREARLAPALFGEGIAPWHPAGGKGVGEILSLTWRNGFGFRWNRASLTP
                     KSRFVYAGEGWALTMLGNTLVMSDGTATLRLLDPATLHLRRLLTVTDAGRPVPMLNEL
                     EAVDGEILANIWLTDRIARIDPADGHVIGWIDVAALHQASGATGPDQVANGIAWDAAH
                     RRLYITGKEWPALFEIAPPKGR"
BASE COUNT         2269 a         5228 c         5439 g         2494 t
ORIGIN      
        1 aatctcgcgc cagctttcat acgggcggat cgagcggctg cggtcggtca accccagcca
       61 gatggggccg tgcttcttga taagggcgcc cagcgtgcaa tcgtcgatca gcgcgccgcg
      121 catggcggca atgccgccgg ccgcctccag cgtcttgcgc ttcaccagca cgcagccgcc
      181 cgccgcgccc gccacacggc gggtgaagcg gccccggcgg ttgatccagc cgaaggggta
      241 gagcatcatg aagaaccaga tgaaggcggg gatcagcagc ttttcggccg gccgcgcgca
      301 atgcagcttg gccatgaagc tgaccagccg ccgatcgccg ctgcgggcaa tgcccaccag
      361 cgtggaaagc gtgtcgggcg tgtgctcgat atcggcgtcg gtcagccaga gataatcggg
      421 cgtgccggcc gcggcgatcc cctggctgac agcccagagc ttgccggtcc agccatcggc
      481 cagcggggcg cctgtcagca ccgtcagccg ctctgccccg tccagcgcgc gggcgatggc
      541 gccggtgccg tcggaggaat tgtcgtcgac cagaacgatg cggaacgggc ccttgtagtc
      601 ctgcgccagc aggctgccga tcgagcgggc gatgacatcg gcctcgtcgc gcgcgggcac
      661 tacggccacc actgcgggca gcggcggggt gagcaggccg ggcgtgcgcg tgtcgcgctg
      721 ggcgcaggtc cagaagccgg tggcggcaag gcccagccac accgccagta ccacaaggcc
      781 aagcagcagc atcagccgat catccctgcc gcgcggaacc aggccagcgc atcggcgatc
      841 gcctcctgat aggggcgggt ggcatagccc agctctgccc tggcgcgcgc gctggaataa
      901 tacatgtggt ggccggccat gcgcagcgcg tccagcgtga ggaaaggttc cttgccggtg
      961 atccgcccgc ccagttcggc cgcataggcc agcgggaaca gcggcgcgcg cggcagcttc
     1021 acacgcggcg gcttgcggcc caccatggcg cagatgtcgg ccagcatctg gcccagcgtc
     1081 acatcctcgc cgcccagcac atagcgccgg ccgatctggc ccttctcggc cgccagaata
     1141 tgccccagcg ccacatcatc gacatggacg agattgaggc ccgattcgac ataggccggc
     1201 atcttgccat tggccgcttc caccagcacg cgcccggtgg gggtgggctt gatgtcgcgc
     1261 gggccgatcg gcgtggaggg gttgacgatg accgccggca ggccgcgcgt ggcgaccagc
     1321 tcttccacca gcctttcggc caccactttc gagcgcttgt agatgcccac cgcctgttcg
     1381 gggctggccg ggcgggtctc gtccgagggt tcgccatggc cgggcagcag cgtggcaaca
     1441 gaagaggtgt agaccacgcg gccaacgccg gcggccagcg cgccttccat caccgcgcgg
     1501 gtcatggcaa ggttgttgcg caggatctcc tccggatcgc gcgcccacag gcgatagtcg
     1561 gccgctacgt gatagagagc gccggcgccg gccatcgccc gggccacgcc ggcctcgtcg
     1621 cgcaggtcca cctcgatcag ctcgccgggg aaatcggcca gattgccgcg cgggctgctg
     1681 gcccgcacga ggcccacaag gcggcgcccg cgcgctgcca gctgccgggc gactgccgag
     1741 cccagaaagc ccgatacgcc ggttacaatg gttgtctgcc cgtccacgcc gcgcttccta
     1801 gggggcaaat cgccgcgccg caacacgcca cgtgcagccg ggggcaaatc ggcagcggtt
     1861 gttgcggcag gcggcggcac gcttgcataa agcgcgctgg ccagcggcgc gcaagctggc
     1921 taagggcggg gcccatggtc ttgagcgtga tcctggcacg ggcggtggcg gggctggtgg
     1981 tggtgctggc cctggtggcc attgcctatc agctggccgc gctggcgctg ctgcgccggt
     2041 ttttcgcgcg ggcggcaggg gctgcggcgc cggcgcattg gccggcggtc agcctgctga
     2101 agccgctaca tggggcagag ccgcgtcttg gcgccaatct cgccagcttc cttgcgcagg
     2161 attatcccgc cccggtgcag atggtgtgcg gcgtcaatgc gctgggcgat ccggcggtgg
     2221 gcgcgctgaa ggcgctgagc gcgcgccatc cgggcgccca tatcagcctg tccaccggcc
     2281 cgcgccagcc cggcgccaat gccaagatgg gcaattgcgc cgctttctat ccgctggccc
     2341 gccacgatgt gctggtgctg tcggacagcg acatggtggt ggggcgcgat taccttgccc
     2401 gggtggtggc ccagctgctg cggccgggcg tgggggcggt ttcctgcctt tatgtggggc
     2461 gcggcgatgc cggcgtgtgg agccgcatcg gcgctgccat catcagctgg cagacgatgc
     2521 ccaacatggt gctggggctg gcgctgggca tggcgcggcc ctgcatgggc tccaccattg
     2581 ccatccggcg cgaggtgctg gaggcgattg gcggctttgg cgcctttgcc gatatactgg
     2641 ccgatgatca tgccattggc gcggcggtgc atggctgcgg gctggaagtg gcgatcccgc
     2701 cgatgctgct ggtgcatgcc ggggcggaag gttctgcccg ggtgctgtgg cgccagcatc
     2761 tgcgctgggc ggccaccgtg cgcgatctgg cgggggcggg ccattatggc tctgttgtca
     2821 cccatgcgct ggcgctggcg ctgctggcgg cgccttgcgt ggcggtggcg ggcctgccgg
     2881 ccgcctgggg ggcagggctg gtggcagcgg cgctggcggt gcggctggcg ctggccggcg
     2941 atgtggcccg ccgcatgaag cggcttgacg gcgcggagca attgcctttc tggcttctgc
     3001 ctgtcgcgga tattttcgca ttcgccgtgt ttctggcgag ctggagcgcg cgaaagattg
     3061 attggcgcgg gcttccactt actataacgg gctccggacg catcgcgcat agcggcgccc
     3121 ccagcaatga ttgagagact gatgaagcgg acccttttcc tccaggcccc ctctttcgac
     3181 ggctacgacg gcggtgccgg cgcgcgctat caaatgaagc gtgaggtcaa gagcttctgg
     3241 tatccgacct ggatcgccca gcctgctgcg atggtgcccg gatcgaagct gatcgatgcg
     3301 ccctcgcacg atctctcgtg ggaagacatc gcccatgagt tcgacgagcg cgacctggtg
     3361 attctgcata cctcgacgcc ttcgttcaac caggatgtgc gcacggccga gctgatcaag
     3421 gcccgcaacc ccaacatcca gatcgccttc gtgggcgcca aggtgatgat cgaggacgag
     3481 aagagcctgg ccgccagccc ggcgatcgac ctggtctgcc gcgaggaata tgatttcacc
     3541 tgcgtcgact tggccgaggg caagccgctg gccgagatcg acggcatcac ctggcgcgcg
     3601 cccgatggca gcatcgtgcg caacaaggat cgcaaggcga tcgaggacat ggacgtgctg
     3661 ccgatggtct cgcccatcta caagcgcgat cttgacagca cgaagtattt cggcggctat
     3721 cagcgccacc cctatgtctc gttctacacc ggccgcggct gcaagagccg ctgcaccttc
     3781 tgcctgtggc cgcagacgat ctcggggcat aactaccgct atcgctcggt gcccaaggtg
     3841 atcgaggaag tgaagtacat cctcgccaac atgcccgaag tgaaggaaat cttcttcgac
     3901 gacgacacgc tggccgatgc gcatgagcat gtcgtggcgc tcagccgcga gctgcgcaag
     3961 ctgggctttg gcaccaaggg tttcgacaaa acctggggct gcaacgccaa ggccaacgtg
     4021 ccctatgacg tgctcaagga aatgaaggca ggcggctgcc gcgtgctgct cgtcggctat
     4081 gagagcggca accagaagat cctgcacaac atcaagaagg gcctgcgcac cgatgtggcg
     4141 cgtcagttca ccaaggatgc gcattccctg ggcctgacca tccacggcac cttcatcctg
     4201 ggcctgccgg gcgagacgct ggagacgatc gaggaaacga tccgttacgc catcgagctg
     4261 aacccgcaca ccattcaggt gtcgctggcc gcgccctatc cgggcacgtt cctctacaag
     4321 caggcgaccg agaacggctg gttcgacggc accgatcacc tgctgaccga tggcggcaac
     4381 cagatcgccc agctctccta tccgcacctc tcctccgccg tcatcttcga caaggtggaa
     4441 gagttctatc gccgctacta tttccgcccc agcaagatcg cctcgatcgt gggcgagatg
     4501 ctgcgcgact gggacatgat gaagcgccgc ctgcgcgaag gcgtggagtt cttcgacttc
     4561 ctgcgccgca gaaaggaagc agcctgagca tcacggccgg cgttgcacgc gggcagacgg
     4621 cgcagggtgg tgacgggggg gccggaccgc gcagacgcct gattgtgacc gccgatgatt
     4681 tcggcgccgc actggcggtg aacgaagcgg tggagcgcgc ccatgtggat ggcatcctca
     4741 ccgccgccag cctgatggtg gcaggcgaag gggccgcaga tgccgtggcg cgtgcgcgcc
     4801 gcctgccggg ccttggcgtg gggctgcatg tggtgctggt ggaagggcgg ccggtgttgc
     4861 cggcagccca ggtgccggcg ctggtggggc cagacgggca gtttgccccg aacatggtgc
     4921 gcaccgcctt tcgcattgct ttcagccgcc gcgcgcgcgc gcagatgcgc gccgaggtgg
     4981 ccgcccaatt cgctgccttt gccgccactg gcctaccgct ggatcatgtc aatgcccaca
     5041 agcatttcca cctgcacccg atgatcgccg cggccatcct tgaggccggc cgggcgcatg
     5101 ggctggcggc catccgcgtg ccgcgcgagg gcggcggcag cggccttggc gcgctgctga
     5161 tgcgctggtg ggcggggctt ttgggccggc gctggcgccg cgccgggctg gcgacgaatg
     5221 atcaggtggt gggccttgcc gtcagcggcg ccttcacgcc cgaacggatg cgcgcggcgc
     5281 tggccagcct gcccgagggc attaccgaga tctacaccca tccggccacg gccgatgcct
     5341 atccgggttc ggcgccgggc tatgcctatc gcgcggaact ggccgcgctg gttgatcctg
     5401 tcgtgtgcaa gctggccggc gctgtggcgc gcgggccgtt tcgcgccctt gccaccctgt
     5461 catgcccttc ggggtcacat tcttctgcta tcgaggccgc ctgagcatgt ttgccaccca
     5521 gcttgtcggt cttggtctga tggcagcggg cgtgccgccc gaaagcgccc tcgaaagcgc
     5581 caatgccgtg gtgccggtgg cagaggccgc gcccgcagcg gccccggcgc cggtggtggc
     5641 ccccgcatcg gcccagcccg cgccggccca gcctgccgcc gccgatacca cgccgatcga
     5701 cgatcccgaa acggcggcgg ccatccacca catggcctgc gaggcggttg ccaccggcga
     5761 tgacgacgaa ggccaactgg ccgccgatgc cggcgtggac ccggcccagg cggtctgccc
     5821 gccgccgccg cccaccgatc ccgatccgct gaagggcttc aaccggctga gctttgccat
     5881 ctccctgccg atcgaccgct atatcgtgcg gccgctggcc atggtctaca aggcggtgct
     5941 gcctgcgttc ctgcgtgatg gcatccataa cgcgctggaa aacatctatt tccccaccat
     6001 cctggtgaac gacatgctgc agctgcgccc ggatctcacc ggcaagacgc tgatgcgctt
     6061 cttcgtcaac agcacgctgg ggctgggcgg cctgttcgat gtggccaagc gcaagccgtt
     6121 caacaccccg gcccatgcca acagctttgg cgacacgctg gggctgatgg gcttcgaggc
     6181 gggcccctat ctctatttcc cggtgatggg gccttcttcc acgcgggatc tggccggcat
     6241 gatcgccgat gccttcaccc agccggtgct gttcgattat gtctcgcaca cccatgtgaa
     6301 gacggtgcac cagcgcgagc gctcttttgt caccagcacg atctcgctga ccactgccgg
     6361 cgccagcgcg ctggtggccc gcgggctgga cgagcgcgtg cgcgccgatg ccgatctcaa
     6421 ggcgctgaag gcgcaatcgg tggatttcta caccaacatg cgcctctctt acctgcagaa
     6481 ccgcgaggcg gagattgccg agctgaagac gcgcggcggc aagcccgccg atgtgcccgc
     6541 cttcgacgag ccgctggaag atccgggcgc ggtcaaggcg ccccagcagc cgcctcagcc
     6601 ccagcccaac tcccaggcca agccttaagg gtcaggccag cgcggcccgg gggcgggcct
     6661 gctggcggcg ggcgcgccat tccatcgccg gccacagcag cagcgcggcg ccaaacgcca
     6721 catcgcgcag ccgcagcacc aggctgagcg ccagcgccat cggcgccggc acgcccagcg
     6781 ccgcgcctgc cagcaccgcg cccgcttcct gcatcaccag cccgcccggc acgaagaaca
     6841 ggatgacgcg ggccgaagtg gccgcgctct cgatcgccag cgccgagaac agcgagaggt
     6901 gcaggccaaa cacgccggcg gccagccaca cctgcacgcc cgacaggccc cagccgatga
     6961 catggaggcc aatcgccagc ggcaggccgg tgcgctggcg gtgcatctcg cggcgcagca
     7021 ggtgccattc ggcggcgatg cgccggcgca ggcggtggcc ctgcggcagg ccgcgccacc
     7081 agcgccgcgc cgccagcccc accagcagaa gcaccagcgc caccatgccg ccggccacca
     7141 gccagccatg ggccgccgag gcgccgccgc cagaaggcag cccggccgcg ccgtgcccac
     7201 ccagccagaa gccggccacc acccagccgg ccagcgccat atagggcagc tctgccagca
     7261 cttcggcggc cacatccagc acccgcagca tcagcgccgc gcgggccttg ccgccggcca
     7321 gcaccagcat gcgcgcgccg atcgcctcgc ccatgccggg ggggaagatc agcaggttgt
     7381 gcccggcatc gcgggtgagg cgggcgagga agctggcgcg gaagggcacg ccgatgttga
     7441 gcgcctgctg ggcggccgtg cacaccagaa tggccaccag ctgcaccggc agggtaacca
     7501 gcaccgccag cggatcgatc tgccgggcgg cctgggcgaa caggctgcgc aggcccggat
     7561 tggccgccac cgaagcaagg cccgccacga gaatcaccgc cggcaccgcc cagccatgcc
     7621 agcgcgggcg agaggcgggg gcaggggcgg cagggctggc gggctgggtc atggctttgc
     7681 gccttaggcc gggcggggcg cggctgcaat ggggctgcaa tggggccgca gtggggccgc
     7741 aaggcggcgg gtggcgcgtg gtcgtatagg cgcagcacgg gggttgtcgc gcgggggaat
     7801 ttcggccaag gcggcccccg atgtatatcg cccgtctcaa ccaggccatt ctggccgccg
     7861 cccgccgccc gcggcttgtt ctggccggcg ttctggcgct ggcggcagcg gcgctggcgg
     7921 tggtgatcgg cgatttcgac atgaccaccg ataccggcga gctgatctcg gccagcacgc
     7981 cctggcggca ggatgagagc cgggtggaaa acgccttccc gcagctcaag gattcggtgc
     8041 tgatcgtcgt cgatggcgcc acgcccgagc tggccgagga tggcgccagt cgcctcgctg
     8101 ccgccttggc cgacgacaag gcgcatatcg tcgatgtgaa gcgccccgat ggcggcccct
     8161 tcttcgaccg cgaggggctg ctgttcgcct cgcttgccga tgtgcgcgag gccacccgcc
     8221 ggctgatcga tgcgcagccg ctgctgggcg ggctggcgtc agaccccagc ctgcatggca
     8281 ttgccgccac gctggccacc gtggccgatg gcgccgccaa gcccggcgcg gccccggccg
     8341 accagctggc aacgccgctc gcccgcttgt cctcggcgat cgacgaccgg ctggccgggc
     8401 ggcatgtgtt cttctcgtgg cagcgcctgt ttgccgatgg cgccgggccg ctgagcccgc
     8461 ccatccgccg cgtgctggtg gtgcacccca ggctcgattt cagcgatctg gagcccggct
     8521 ctgccgcggt ggacgcgatc aaccagaccg ccgcgcgcct gcagatcgat gccgcgcacg
     8581 gccagcatgt cggcatcacc ggccaggtgc cgctggccga cgaggaattc ggctcgatcc
     8641 gcgagaatat cgggctggtg ggcgcgctga tggtggccgc catggtggtg tgcctgtggc
     8701 tggccacgcg cagcgccagg atggtgggcg cgatcatggg cacgatcgcg gtggggctgg
     8761 tcattaccct cgcgctgggg ctgctggcgg tgcaccggct gaacctcatt tcggtggcct
     8821 tcatcccgct gttcgtgggg ctgggggtgg atttcggcat tcaggtcagc gtgcgcttca
     8881 atgccgagcg ggccgccggc gccgagccga tgcaggcgct ggaacgcatg gccgcggcca
     8941 ttggcgagcc gttgctgatg gcggcgggcg cgatcttcct cgcgctggcc gcgtttctgc
     9001 ccaccgccta tatcggcatt gccgagctgg gggtgattgc cggccttggc atgatcgtcg
     9061 ccttcgggct gaacatcacg ctgctgccgg cgctgctggt gctgctgaag ccggccgcgc
     9121 ccgcccaccg ggtgggctgg gccggggcgg cggcggccga tcgctggctg caccgccatc
     9181 gcaagggcat tctcgccagc tttgccggcg cggtggtggc caccattgcc gccctgccgc
     9241 tggtggcgtt tgatttcaac ccgctgcacc tgcgcgatcc cagggcgccg gcaatggcga
     9301 cccttgccga tctgatgcgc gatgccgatc gcacgcccaa caccatctcc atccttgccc
     9361 ccgatgccgg gcaggccggc gcgctggccg cgcgccttgg ccgcctgccg gaagtggccc
     9421 atgccgtcag catcgacagt ttcgtgcccg aggggcagcc ggacaagctg gcgctgatcc
     9481 aggatgccag cctgctgctc gatgcggcga tcaacccctt cgacctgccg accagcgccg
     9541 atgataccgc aacgcaggcc aagctgcatg aggcggccga ccggctgcgc gcgctggctg
     9601 ccgcccggcc gggcgcgctg ggccgctctg ccgcggcgct ggcggtcagc ttcgagcggc
     9661 tggccgcggc cagccctgcc agccgcgccg atatcgccgc catgctggaa atgccgctgg
     9721 cgatcacgct cgacaagatc cgcgcctcgc tgcaggccgg cgaggtgagc cgggccagcc
     9781 tgccggccga tctggccgcc gactgggtgg ccaaggacgg cagcgcgctg gtgcaggtga
     9841 caccggccgg caacagcctc gacaaccgcg tgctcacgcg cttcaccgcc gccgtgcggg
     9901 cgcaggcgcc ccatgccagc ggcctgccgg tggcaacgca agaagccgcg cgcaccgttg
     9961 ccggcgcttt tgtgcaggcg ggcgtgctgg cgctgctgct ggtgagcggg ctgctgtggc
    10021 tggtgctgcg ctcggtgcgc gaggtggcct ttaccctggc gccggtggtg ctgtcgggct
    10081 ttctcacgct gggcagctgc gtgctgatcg gccagccctt gaattttgcg aatatcatcg
    10141 catttccgct gttgttcggt gtgggcgtgg cgttccacat ctattttgtc atggcatggc
    10201 ggcgaggcgt ggaaaatctg ttgcagacaa gccttgcccg cgccgtggtg atgagcgcgc
    10261 tggccaccgg cagcgccttt ggcgccctgt ggctgagcca ccaccccggc accgccagca
    10321 tggggctgat cctgatgatc tcgctgatct ggacactggt gtgcgcgctg gtgttcgagc
    10381 cggcgctgct gggcccggtg catgggcccg cccgcgagga tgtgtgatgg gcgcgccccc
    10441 cgccactgac ctggctgcct ctgatgccgc cgatccgcgg gcaaggctgc tgctcgcttc
    10501 gatccacgat gtgggccccc gcttcgagcg cgaggtggac cagctggccg agcggctgga
    10561 tgggcatctg ggcagtgcgc gctttgccat gctggtggtg cccgatcact ggggggcagc
    10621 accgctgctc accgccggcg gctttcaggc caggctgcgc ggctgggccg atgccggggt
    10681 ggagatgttc gtgcatggct ggttccaccg cgacatggct gcccatgccg gccttgccgc
    10741 cttcaaggcc cgccacatga ccgccagcga gggcgaattc ctgggcctgg cgcaggagga
    10801 agccgcccgc cgcatggatg aggggcgcaa gctggtggaa gatatcatcg gccggccggc
    10861 cgccggcttc attgcccctg cctggcttta tggcccgggc gcccgcgcgg cgctggccac
    10921 cagcggcttt gccctggccg aagaccatct gcgcgtgtgg cagccgggca ctggccgcgt
    10981 gctggcgcgc gggccggtga tcacctgggc cagccgcagc aaggcgcgcc agcgttccag
    11041 ccgtttcttt gccggccttg cccgccatgc gctgcgcggc cagcaggtgg cgcgcattgc
    11101 cgtgcacccg ggcgatacca ccgtgccggc gctgctttct tccatcgacg cgaccatcgc
    11161 cgctttcacc cgcgcccacc ggcccggccg ctatgccgaa cttttgccgg cccggccctg
    11221 tcaggttcag gcggcattat gattgagcgc actacgcaac cctttggcgg ggacgaggcc
    11281 agcgacaggc ctgcccgcgc ggaatcggat atggcacaga tgtaccagca cagttttgct
    11341 gccggcgagg gcagcccgga aacccggctc gacatgggca cggtggaaca gcgtcagcgc
    11401 ggatcacgcc tggccggcct gttcagcgcg gcgatctcgc tggccatgct ggtggctgtg
    11461 gcgatggaga tccgccagct gcatttccgt gaaattctgg cgatgatccc gcgttcggcg
    11521 ccgttctggc tggcctttgc cgcctattat ctggtgccgc ctttcaccga atggacgatc
    11581 tatcgccggc tgtggggcct gcccctgtcg ggcgtggcgg cgctgctgcg caagcaggtg
    11641 tcgaacgagc tgctgctggg ctatctgggc gaggtgcagt tctatgcctg ggcgcgcgcg
    11701 cggctgaaca tggtggccgc cccctttggc gccatcaagg atgtgacgat cctttccgcg
    11761 ctcaccggca atatcgccac gctggtgatg ctggtggggg cctggccgct ggtcatgtcg
    11821 ggccagctgg ggatggaatc gcgcacggtg ttcatgtcgc tgggcgtggt gctgctcacc
    11881 agctgcatca tcctgttctt ccgccagcgc ctgttcagcc tgcacaagcg cgatctgctg
    11941 atcatcaccg gcctgcattt tggccgcatc atcatcatcg tgggcctgtg ggcgctgatg
    12001 tggcacctgg tgctgcccga ggtggaactg gcgatgtggc tggtgctggc caccttgcgc
    12061 atgctgatct cgcggctgcc gctggtgccc aacaaggatg tggtgtttgc cggcgttgcg
    12121 gtgttcctcc tcggccatga ggcgcagatc tcgcatctta tcgccatgat ggcggggctg
    12181 ctgccggtca cccatatcgt cgtcggggcg atcttcgcct cggttgatct gttccaatcc
    12241 agaaaggaaa gctgagcgat gcgcctctct cctgcattgc tggccggttt ggcggccacc
    12301 tctgtgctgg ccgcggggcc ggcgcaggct ggcgacaagc cggtggtgcc gctgccctcc
    12361 acgcccgatc tcggcaaggc cgaggcgcat tgccgcgcgg gcgagcaggg cccggcgctg
    12421 ctgatctcgg tggtggggct gaaggaccgc gccggccggc tgaagctgga ggtctatccg
    12481 tcgaacgacg aggatttcct gatggacgac aatgtgctcg tctatcagca caaggccttc
    12541 cgccgggtgg aggaagaagt gccggccagc ggcgctgtgc agctgtgcgc gcgcattccc
    12601 ggccccggcg cctattcggt gatgctgctg cacgaccgcg acagcaaccg caagttcggc
    12661 tggatgatcg atggcatcgg cttttcggcc aatccgcggc tgggctggca caagcccaag
    12721 gcagccgcca cgcgggtgat ggccggcacc ggcctcacgc ggatcaacat cgtgctcaat
    12781 tatcgccacg ggctgggcat gtcgcccatg cgcgacgggg attgatcggc cgcaaggccg
    12841 gatgcccgcc gatctgctga gttgcaacgc cgggagatgc ggatgagaat cgtcgatgtc
    12901 tgcgcctttt attcgcccca gggcggcggc gtgcgcacct atgtcgaaca gaagctggcg
    12961 atcgggccgc agctgggcca cgagatcatc gtgctggcgc cgggagaccg cgccgaggtg
    13021 atcgacaggg gcccgcgcgc gcgcatcgtg acgatcccgg cgccgcgctt cccgctcgat
    13081 cgcaaatact ggtatttcaa cgatgagccc gcgctgcatg cggcgatcag cgggctggcg
    13141 ccggatttcc tggaagtctc ctcgccctgg cgcagcgcgg caatggcggc gcgctggccg
    13201 ggcacggcgc cgcgcgcgct ggtgatgcat gccgatccgc tgtcggccta tgcctatcgc
    13261 tggctgcagc cgctgttctc gcgcgagacg attgaccgcc agttcgcccc tttctggaag
    13321 cacctgcgcg agatgggcaa gagctatgac cgggtgatct gcgccagccg cgagctgaag
    13381 gagcgcctgg cgcgcggcgg cgtgagccac acctcgctgc atccgatggg gatcgaggcg
    13441 ggcgttttct cgccagagcg gcgcagcccg gcgctgcggc ggcgcctgct ggcgctgtgc
    13501 ggcctgccgg aaagcgcgcg gctgctgatc ggcgtgggcc gcctttccgc cgaaaagcgc
    13561 tggccgatgg tggtggatgc cgtcactgtg gccagccagt cgatgccgat cggcctggtg
    13621 ctggtgggcc aggggcggca gaaataccgg atcgagcggc agatcttcgg caacccccat
    13681 attcagctgc tgggcgtgga aaaggaccgg cagaaattcg ccacgctgct ggccagcgcc
    13741 gatgcgctga tccatggctg cgaggcggaa accttctgca tggccgctgc cgaggcgcgc
    13801 gccagcggcg tgccggtgat cgtgcccgac cggggcggcg ctgccgaaca tgcgcgcggc
    13861 ggggcgggga tcacctatcg ttcgggcaat gcggtgtcgg ccgcgcatgc ggcgctgcgg
    13921 ctgctgcaca agccgccgcg cgtgcccttt gccgatgtgg tgacgatgga ggatcatttc
    13981 gccggcctgt tcgccgaata tgcagcgctg ctggccgggc gcagcgcggc ggcctgaagg
    14041 ggcagcgcgg cggcgcttaa tgcgcgtcta tgcggctgag catgcggaag aagaaagagg
    14101 tggaccagcc cagcaggatc acccccagaa tgctttcgaa agcgcccagc aggcgccagg
    14161 ccagggtgat gtgggcatcg ttgtagccca cggtggaata gctgatggtg gagaaataaa
    14221 gcgcgctttc aaagccgcgc accgcgcctg tggccacata gacaagcgcg aaggcccaga
    14281 tctccacccc gtgcagggcg atgatcgaga tgacgatggc cagggtgaag gccaccccgc
    14341 gcggcgataa cgggcggata tgttgcagcc gctcgctgct ggcctcgctg cgcagggcgc
    14401 gggtgaggcc gaacaggccc atgccatgca cgcatacgca aaacaccacc atcagcgagg
    14461 agacggcgaa ttccgcgatg agattggccc atccgcccgg cggcatcgtt aatttgcccc
    14521 ccgcggcatg gttcagcggc ccttcggcgg ggcgatctcg aacagcgccg gccattcctt
    14581 gccggtgatg tagaggcgcc ggtgcgcggc atcccaggcg atgccattgg ccacctgatc
    14641 gggcccggtg gcgccagagg cctggtgcag ggcggccacg tcgatccagc cgatcacatg
    14701 gccatcggcc ggatcgatgc gggcgatgcg gtcggtcagc cagatattgg cgaggatctc
    14761 gccatccacc gcttccagct cgttcagcat gggcaccggg cggccggcgt cggtcaccgt
    14821 cagcaggcgg cgcaggtgaa gcgtggccgg atcgagcagc cgcaaggtgg ccgtgccatc
    14881 cgacatgacc agcgtgttgc ccagcatcgt cagcgcccag ccctcgccgg catagacgaa
    14941 gcggcttttg ggcgtgaggc tggcgcggtt ccagcggaag ccaaagccat tgcgccaggt
    15001 gaggctgagg atctcgccca cacctttacc gcccgccgga tgccaggggg cgatgccctc
    15061 gccgaacagg gccggggcca ggcgcgcctc gcggatcacc cggccgcttt ccagatccac
    15121 ctggcggatg gacgagcgcc cctccatccc ggtgctttcc catagctcgc cgcggtcgat
    15181 gaacaggccc tcggtgaagg cggccggatc atgcggcagg cggcggattt cccgcgcggg
    15241 caccacgggc gcggcgctgg ccggggcgtg gggggcgctt tgcgcgggca ggcgcgccgg
    15301 cgccgcgctg gccatcgcca gggcagcacc caacagggcc ggccacaggg ccagcgccgg
    15361 cgcgcggcgg cgcatcagtc gcgcagcagt tcgttgatgc cggtcttggc gcgggtctgg
    15421 gcgtccaccg
//
LOCUS       DASRYE010000059        11204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_215470,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000059 DASRYE010000000
VERSION     DASRYE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215470"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFF98_03220"
     CDS             <1..480
                     /locus_tag="VFF98_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZU62670.1"
                     /translation="GNAKAVPLPRPLLGSAEPHFSFAGLKSAVLRAHEAGSHAPEDIA
                     AAFQQAAIDCLLDRTRRALAAARALSPVPVSALVVAGGVAANKAIRTALEGLAAAEGL
                     PFTAPPLPLCTDNAAMIAWAGYERFALGQTDGLDVAARPRWPLDPDAAPARGAGVKA"
     gene            477..1508
                     /locus_tag="VFF98_03225"
     CDS             477..1508
                     /locus_tag="VFF98_03225"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZU62671.1"
                     /translation="MSPAIGVIGAGAWGTALAAMLAADGTSVALWARGAALAEEINAQ
                     RTNATYLPGVALPPAICATADLADLAAVPALLAVVPSQFLGGVLAQLPAVPRDLVLCA
                     KGIEAGSHRLMVEVAQEALPGAGLAVLSGPTFAREVACGLPTAVTLACAGGEAQWQRL
                     SPLIARPHFRPYYSADVVGAEVGGAVKNVLAIACGVVEGLALGENARAALIARGFAEM
                     LRFGTALGARGETLSGLCGLGDLVLTCASVASRNYALGKALGAGQSAAEALAGKASVA
                     EGAASAPVLAAMARDRHIAMPIVDAVCRLLRGEAPARQVVSELLARPLRAELAPAAPA
                     DGAPGRSAE"
     gene            1541..3058
                     /locus_tag="VFF98_03230"
     CDS             1541..3058
                     /locus_tag="VFF98_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU62672.1"
                     /translation="MPAADGNCAAQAAGQRDIAALAKGGRTNFFGFLLRLAARLPFLF
                     IAGRLYGAAALGRFASALVIIELTCQLCTLGQKRGIAQRLAEEARHDAHVVADGLVLS
                     GLFAIAVSVVLWFIPEPMFPAGHFTQLDRLLPLSILPYTLTEIALAALAYRFDVGATV
                     KSRSVVEPWTLSIAAGAFYYISPDSGLSLSYIVSIYAAAAAALWPLLRSYGWPSQWRA
                     HPLRIARLAIDTAPLAVADAVEWGTRRLDIAILGIFATPTSVGVYYVAQQVASLPQKL
                     KTSFEPILGPVIARNLKIQNHAAIAAQVCQVGFWITAAQAGIGLALGIPGGGVLGLVG
                     PQFVGGLGALAFLLGAEVGAATSVVSEAALIYIAPMPNLWISVGTIVLQAGLTVAGMW
                     IDRSFALDEMDAAASAAGALMVTLSIASIVKSRLLAHYLKTAITTWRWPLVLASALAS
                     LVGLLACDALPEWAELAFGIPAILGVYCLVIWHFGFGPEDRLLFRRDAARPRQVA"
     gene            3055..3834
                     /locus_tag="VFF98_03235"
     CDS             3055..3834
                     /locus_tag="VFF98_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZU62673.1"
                     /translation="MSGAAGLAGWRGRLRALAAMLAGWLVVAGLGAAADMLWGPGLIA
                     GLQRGADAAIAAAGAGPGITARFVDSHGWQTRHPQLQGVERIDEQRRLLLAQAVAAVP
                     GMGGVRWAAVPAADAGPDAAPPAVADCQSHVQAILATRTIRFAEGRATIDPASRELLD
                     EVAAALRPCAGGVIAITGHTNASGDAETNLALSRERALAVRNALALRGIDLAGLRARG
                     VGSAQPLPGLTADDPANRRIEFSVIAPVSLEPTVVDTPGPY"
     gene            4004..4573
                     /locus_tag="VFF98_03240"
     CDS             4004..4573
                     /locus_tag="VFF98_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62674.1"
                     /translation="MLTLIETNWPVFAAALLFGLLVAYLLWGRARAPRERHRAPDVLD
                     EGAEAARRNQALIDAPSAASALHSAHATEAARVAAPLAGTGPGIMGGIGELLAAAATH
                     EVAAAKDAEGDDLSRLKGVGPKLKARLAELGVTSFAQIAAWQDADIAAIDAQLGSFAG
                     RATRDQWVAQASFLAAGDIAGYEAQFGKL"
     gene            complement(4632..6818)
                     /gene="uvrB"
                     /locus_tag="VFF98_03245"
     CDS             complement(4632..6818)
                     /gene="uvrB"
                     /locus_tag="VFF98_03245"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZU62675.1"
                     /translation="MAELIIRRGLEEPDTSGSFTPHKPARPAKAEDSRPLRVVSDYTP
                     SGDQPTAIAELVEGIAAAEQTQVLLGVTGSGKTFTMAKVIEALGRPALILAPNKILAA
                     QLYGEFKSFFPDNAVEYFVSYYDYYQPEAYVPRSDTYIEKESSVNEAIDRMRHSATRA
                     LLERDDVIIVASVSCLYGIGSVETYSAMIFDLKKGENADQGEIIRKLVALQYKRNDVA
                     FQRGAFRVRGDNLEIFPSHYEDMAWRISFFGDEIEEIAEFDPLTGKPGVKLEKVRVYA
                     NSHYVTPGPTMKQASEAIRFELAERLKELEAEGKLLEAQRLEQRTNFDLEMIAATGSC
                     AGIENYSRFLTGRLPGEPPPTLFEYLPDNALLFIDESHQTVPQIGAMARGDHRRKITL
                     AEYGFRLPSCIDNRPLRFNEWDVMRPQTVAVSATPGGWEMEQAGGVFAEQVIRPTGLI
                     DPPVIIRPVEDQVQDCIAECRKTAEAGYRTLVTTLTKRMAEDLTEFMHEAGLKVRYMH
                     SDVETLERIELIRDLRLGVYDVLVGINLLREGLDIPECGLVCILDADKEGFLRSETSL
                     IQTIGRAARNVDGRVILYADRMTGSMERAIAETDRRRARQQAYNEEHGITPATIKRHI
                     HDIVADTAARDGVLVEIEDDATNNLVGHNLRAYIEELEKRMRNAAADLEFEEAGRLRD
                     EIRRLEASELGLPEGEHKAPLVGRSNEGKPGTRKTRYGKVQKKWGK"
     gene            7001..8434
                     /locus_tag="VFF98_03250"
     CDS             7001..8434
                     /locus_tag="VFF98_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZU62676.1"
                     /translation="MAPSPLSSFDEMIDADGNIRPAYAGYREWLDEQDGKWLQRQSKQ
                     AESFFRKTGITFNVYGEDAGAERLIPFDMVPRIITRDEWSRLAVGIEQRVRALNAFLH
                     DLYHKREIIKAGKLPERMLKANEAFLPQMAGFSPPGGVYTHIVGIDLVRTGPDEFMVL
                     EDNARTPSGVSYMLENRETMMAMFPELFTRVKVQPVSDYPRSLARSLAACAPPACENE
                     RPVVAVLTPGIYNSAYFEHAFLADQMGAELVEGSDLRVVDGRVAMRTTRGWKAIDVLY
                     RRVDDAFLDPLTFNPSSVLGVAGIMDVYRAGGITIANAPGTGISDDKAIYSFMPEIVE
                     FYTGEKPILKNVPTWRCSEEDALKYVLDNLKDLVVKEVHGSGGYGMLIGPTSSKAEIA
                     AFEKKLRAKPDNYIAQPTLSLSTVPIFTKAGLAPRHVDLRPFVLVSPGGINITPGGLT
                     RVALKKGSLVVNSSQGGGTKDSWVLAE"
     gene            8487..9431
                     /locus_tag="VFF98_03255"
     CDS             8487..9431
                     /locus_tag="VFF98_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZU62677.1"
                     /translation="MLGRSAWGIFWMSRYLERAENTARLLDAGFRIALTRGNSTASDE
                     WRSVLVTLGLDEPFAERGREATGPAITDYILRAQDNPTSVLAMMENARTNARMVRTAI
                     TREVWEATNESWMILKDALAKPVREANLGEVLDMIRRQATLVRGAMEGTMLRNDILAF
                     ARAGTYIERADNTARIIDVKYHVLLPSLAWVGSRLDNAQWEMVLRSVAGERAFRWLHA
                     GVLDPRGIAEFLILDARFPRSLAYAYKQLESSMAILSDAYGIETSAHLAVREAVARLA
                     ATNIAGIFDFGLHEFITEAIFTNRQIAEAIAADYRFTE"
     gene            9450..10277
                     /locus_tag="VFF98_03260"
     CDS             9450..10277
                     /locus_tag="VFF98_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZU62678.1"
                     /translation="MRIAISHTTRYRFDEPASYGLQRLRLTPRDCTLQSVVDWHMEIE
                     GGVMEVAYEDHNQNRVALISFGGGAGQVAITCSGVVETRDAAGVAGPHTGFMPLWQLC
                     APTELTRPGPALCALAGRFAAAEPACGTSMIGVLHDLAHEVGEAIAYDKGHTHVGTTA
                     EEALVLGKGVCQDQAHAFIAAVRLLGLPARYVSGYLLMDENAAQEASHGWAEAHVPGL
                     GWVGFDVANDICPDERYVRLAVGADYRDAAPVISQTQGCGEAQLEVAVAVQQRMAMQ"
     gene            10398..11147
                     /locus_tag="VFF98_03265"
     CDS             10398..11147
                     /locus_tag="VFF98_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZU62679.1"
                     /translation="MTYCVGMMLDAGLVLMSDTRTNSGVDNISTFRKMYSWNVPGERI
                     VTLMTSGNLATTQAVVAKLEERTKAPADRSNTLLHAPTMFSIATLIGKLLRETIADRD
                     EDNGLQASGTFSATLILAGQIRGMGTHLYLIYPEGNFIEASFDTPFFQIGETKYGRPI
                     IIRGYDRKMSFEDAVKLMMVSFDSTLKANLSVGMPLDLMVIARDSFEPLHEERITAAD
                     PWYRSISASWSEALRAALDALPDFPFHARGE"
BASE COUNT         1636 a         3627 c         3981 g         1960 t
ORIGIN      
        1 ggcaatgcca aagccgtgcc gctgccgcgc ccgctgctgg gctctgccga gccgcatttc
       61 tccttcgccg gcctgaagag cgcggtgctg cgcgcgcatg aggccggcag ccatgcgccc
      121 gaggacattg ccgccgcctt ccagcaggcc gcgatagact gccttctcga tcgcacccgg
      181 cgcgcccttg cggcggcccg cgcgctcagc ccggtgccgg tttcggcgct ggtggtggcc
      241 ggcggcgtgg cggccaacaa ggcgatccgc acagcgctcg aagggctggc ggcggcagaa
      301 gggctgccgt tcaccgcgcc gccgctgccg ctgtgcaccg acaatgccgc gatgatcgcc
      361 tgggccggct atgaacgctt tgccctgggg cagacggacg ggctggatgt ggccgcccgc
      421 ccgcgctggc cgctggatcc ggatgcggcg cctgcccgcg gggcgggggt gaaggcatga
      481 gcccggccat cggcgtgatc ggcgccggcg cctggggcac ggcgctggcc gccatgctgg
      541 ccgcagatgg cacgtcggtg gcgctgtggg cgcgcggcgc agcgctggcc gaggagatca
      601 atgcgcagcg caccaatgcc acctatctgc ccggcgttgc cctgccgccg gccatttgcg
      661 ccacggcgga tctggcggat ctggccgcgg tgccggcgct gctggcggtg gtgccttcgc
      721 aatttctggg cggggtgctg gcgcaattgc cggcggtgcc gcgcgatctg gtgctgtgcg
      781 ccaaggggat cgaggcgggc agccaccggc tgatggtcga ggtggcgcag gaggcgctgc
      841 cgggcgcggg gctggcggtg ctttcgggcc ccactttcgc gcgggaggtg gcctgtggcc
      901 tgcccaccgc cgtgacgctg gcctgtgctg gcggcgaggc gcagtggcag cgcctgtcgc
      961 cgctgatcgc ccggccgcat ttccgcccct attattccgc cgatgtggtg ggcgccgaag
     1021 tgggcggcgc ggtgaagaac gtgctggcca ttgcctgcgg cgtggtggaa gggctggcgc
     1081 tgggcgagaa tgcgcgcgcg gccctcatcg cccgcggctt tgccgagatg ctgcgttttg
     1141 gcacggcgct gggcgcgcgg ggggaaacgc tttcgggcct gtgcgggctt ggcgatctgg
     1201 tgctcacctg cgcgtctgtg gccagccgca actatgcgct gggcaaggcg ctgggcgccg
     1261 ggcaaagcgc ggccgaggcg ctggccggca aggccagcgt ggccgaaggg gcggccagcg
     1321 ccccggtgct ggcggcgatg gcgcgggatc gccacatcgc catgccgatt gtcgatgccg
     1381 tgtgccggct gcttcggggg gaagcgccgg cgcgtcaggt ggtttcagaa ttgctggcgc
     1441 ggccgttgcg ggccgagctg gcgccggccg cccctgccga tggcgcgccc gggcggagtg
     1501 ccgagtgacc caatcccccc ccctgcctgc cgaccagccg ctgcccgcgg cagacgggaa
     1561 ctgcgccgca caagcggccg gccagcgtga tattgccgcc ctggccaagg gcgggcgcac
     1621 caatttcttc ggcttcctgc tgcggctggc ggcgcgtttg ccgttcctgt tcattgccgg
     1681 gcggctgtat ggcgcggcgg cgctggggcg gtttgcctcg gcgctggtga tcatcgagct
     1741 gacctgccag ctgtgcacgc tgggccagaa gcgcggcatc gcccagcgcc tggcggaaga
     1801 ggcgcgccac gatgcccatg tggtggccga cgggctggtg ctgagcgggc tgtttgccat
     1861 cgcggtcagc gtggtgctgt ggttcattcc cgagccgatg ttcccggccg gccatttcac
     1921 ccagctcgat cggctgctgc cgctgtccat cctgccctac acgctgacgg aaattgccct
     1981 ggcggcgctg gcctatcgct tcgatgtggg ggccacggtc aaatcgcgct cggtggtgga
     2041 gccgtggacg ctgtcgatcg cggccggcgc gttctactat atcagccccg acagcggcct
     2101 gtcgctgtcc tatatcgtct cgatctatgc ggcagcggcc gccgcgctgt ggccgctgct
     2161 gcgctcctat ggctggccca gccagtggcg cgcgcacccc ttgcgcatcg cccgcctggc
     2221 gatagacacc gcgccgctgg ccgtggccga tgcggtggaa tggggcacgc gccggctcga
     2281 tatcgccatt ctgggcattt ttgccacgcc cacttcggtc ggcgtctatt atgtggcgca
     2341 gcaggtggcc agcctgccgc aaaagctgaa gaccagcttc gagccgatcc tgggccctgt
     2401 catcgcccgc aacctcaaga tccagaacca cgccgcgatt gccgcgcagg tgtgccaggt
     2461 gggcttctgg atcaccgctg cgcaggccgg cattggcctg gcgctgggta ttcccggtgg
     2521 cggcgtgctg gggctggtgg ggccgcagtt cgtcggcggg ctgggggcgc tggccttcct
     2581 gctgggcgcg gaagtggggg cggccacctc ggtcgtttcc gaagccgcgc tgatctacat
     2641 cgcgcccatg cccaacctgt ggatctctgt tggcactatc gtgctgcagg cggggttgac
     2701 cgtggccggc atgtggatcg accgcagctt cgcgctcgac gagatggatg ccgccgcctc
     2761 tgccgccggc gcgctgatgg tcacgctgtc gatcgcttcc atcgtcaagt cgcggctgct
     2821 ggcgcattat ctcaaaacgg cgatcaccac ctggcgctgg ccgctggtgc tggcctcggc
     2881 gctggcctcg ctggtgggcc tgctggcctg cgatgccctg ccggaatggg ccgagctggc
     2941 tttcggcatt cccgccattc tgggcgtcta ttgcctggtg atctggcatt tcggcttcgg
     3001 cccggaagac cggctgctgt tccgccgcga tgctgcccgc ccccggcagg tggcatgagc
     3061 ggcgcggccg gcctggccgg ctggcggggc aggctgcgcg cgctggcggc gatgctggct
     3121 ggctggctgg tggtggcagg gctgggcgct gcggcggata tgctgtgggg gccggggctg
     3181 atcgccgggc tgcagcgcgg cgccgatgcg gcgattgctg cggcgggcgc cgggccgggc
     3241 atcaccgccc gctttgttga cagccacggc tggcagaccc gccacccgca attgcagggc
     3301 gtggagcgga tcgacgagca gcgccgcctg ctgctggcgc aggcggtagc cgcggtgccg
     3361 ggcatgggcg gcgtgcgctg ggcagcggtg ccggctgccg atgccgggcc cgatgccgcg
     3421 ccgcctgccg tggctgattg ccagagccat gtgcaggcga ttctggccac ccgcaccatc
     3481 cgctttgccg aagggcgcgc caccatcgat ccggccagcc gcgaattgct ggatgaggtg
     3541 gccgcggccc tgcggccctg cgctggcggg gtgatcgcca tcaccggcca caccaatgcc
     3601 tcgggcgatg cggaaaccaa tctggccctc tcgcgcgagc gggcgctggc ggtgcgcaat
     3661 gcgctggcgc tgcgggggat agaccttgcc ggcctgcgcg cgcgcggggt gggctctgcc
     3721 cagccgctgc cggggctcac cgccgatgat ccggccaacc gccgcatcga gttctcggtg
     3781 atcgcgccgg tcagcctcga gcccaccgtg gttgacacgc ccgggcccta ttaggaggac
     3841 accatgcccc tttgggtgga attgcaggtg ctgctggcct tgacctatgg tgcagggatc
     3901 gccatcggct ggggggtgtg gggccggccg gcggctgccg ccctggcccc gcccgtctga
     3961 ccgcttcccg ccttcccggc tcgccgaaag gatgctgccc ccgatgctga cgttgatcga
     4021 gaccaactgg cccgtttttg ccgccgccct gctgttcggc ctgctggtgg cctatctgct
     4081 gtggggccgc gccagggccc cgcgcgaacg ccaccgcgcc cccgatgtgc tggatgaagg
     4141 cgccgaggcc gcccgccgca accaggcgct gattgatgcg cccagcgccg cttccgccct
     4201 ccacagcgcc catgccacag aggccgcccg cgtggcagcg ccgctggccg gcactggccc
     4261 tggcatcatg ggcggcattg gcgagctgct ggccgccgct gccactcacg aagtggccgc
     4321 cgccaaggat gccgagggcg acgacctctc gcgcctcaag ggcgtggggc ccaagctgaa
     4381 ggcacggctg gccgagctgg gcgtgaccag ctttgcccag atcgctgcct ggcaggacgc
     4441 cgatatcgcc gccatcgatg cccagctggg cagctttgcc ggccgcgcca cgcgcgatca
     4501 gtgggtggcg caggccagct tcctcgccgc tggcgatatc gccggctatg aggcgcagtt
     4561 cggcaaattg tgagatccgg ccggccgggg cagggcgatg gcctccgccc cggcacggcg
     4621 cctgcgcttt ctcacttgcc ccacttcttc tgcaccttgc catagcgcgt cttgcgggtg
     4681 ccgggcttgc cctcgttgct gcggcccacc aggggggcct tgtgctcgcc ttcgggcagg
     4741 cccagctcgc tggcttccag ccggcggatc tcgtcgcgca ggcggcccgc ttcctcgaat
     4801 tcaaggtcgg ccgcagcgtt gcgcatgcgc ttttccagct cctcgatata ggcgcgcaga
     4861 ttgtggccga cgaggttgtt ggtggcatcg tcctcaatct ccaccagcac gccatcgcgc
     4921 gcggcggtat cggcaacaat gtcgtggatg tggcgcttga tggtggccgg cgtgatgccg
     4981 tgttcctcgt tataggcctg ctggcgggcg cggcggcggt cggtttcggc gatggcgcgt
     5041 tccatgctgc cggtcatccg gtcggcatag aggatgaccc ggccatcgac attgcgcgcg
     5101 gcgcggccga tcgtctggat gaggctggtc tctgagcgca ggaagccttc cttgtcggca
     5161 tcgaggatgc acaccagccc gcattcggga atatccagcc cttcgcgcag caggttgatg
     5221 cccaccagca catcatagac acccagccgc aggtcgcgga tcagctcgat gcgctccagc
     5281 gtctccacat cggaatgcat gtagcgcacc ttgaggccgg cctcatgcat gaattcggtg
     5341 aggtcttcgg ccatgcgctt ggtcagcgtc gtcaccagcg tgcgatagcc ggcctcggcg
     5401 gtcttgcggc attcggcgat gcaatcctgc acctggtctt ccaccgggcg gatgatcacc
     5461 ggcggatcga tgaggccggt ggggcggatc acctgctcgg caaagacgcc gccggcctgc
     5521 tccatctccc agccgccggg ggtggcggaa acggccaccg tctgcgggcg catcacatcc
     5581 cattcgttga agcgcagggg ccggttgtcg atgcaactgg gcaggcggaa gccatattcg
     5641 gccagggtga tcttgcggcg atggtcgccc ctggccatcg cgccgatctg cggcacggtc
     5701 tggtggcttt catcgatgaa cagcagggca ttgtcgggca gatattcgaa cagggtgggc
     5761 ggcggctcgc cgggcaggcg gccggtgagg aagcggctgt aattctcgat gccggcgcac
     5821 gagccggtgg cggcgatcat ctcgagatcg aaattggtgc gctgttccag ccgctgggct
     5881 tccagcagct tgccctccgc ttccagctcc ttcagccgct cggccagttc gaagcggatg
     5941 gcctcgctcg cctgcttcat ggtggggccg ggggtgacat agtgcgaatt ggcatagacc
     6001 cgcaccttct cgagcttgac cccgggtttg ccggtcagcg gatcgaattc ggcgatctcc
     6061 tcgatctcgt cgccgaagaa ggagatgcgc caggccatgt cttcgtaatg gctggggaag
     6121 atttccagat tgtcgccgcg cacgcgaaag gcgccgcgct ggaaggcgac atcgttgcgc
     6181 ttgtattgga gggcaacgag cttgcggatg atctcgccct gatcggcgtt ctcgcccttc
     6241 ttgaggtcga agatcatcgc cgaataggtc tcgaccgagc cgatgccata gaggcaggac
     6301 acggaggcaa cgatgatcac atcgtcgcgt tccagcagcg cgcgggtggc tgaatggcgc
     6361 atccggtcga tcgcctcgtt cacgctgctc tctttctcga tataggtatc ggagcgcggc
     6421 acataggctt cgggctggta atagtcgtaa taggagacga aatattcgac ggcgttatcg
     6481 gggaagaagc tcttgaattc gccatagagc tgggcggcga ggatcttgtt gggcgccagg
     6541 atcagcgccg ggcggcccag cgcctcgatc accttggcca tggtgaaggt cttgcccgat
     6601 ccggtcacgc ccagcagcac ctgggtctgc tctgcggcgg cgatgccttc caccagctcg
     6661 gcaatggcgg tgggctggtc gccgctgggg gtatagtcgg agaccacgcg caaggggcgg
     6721 ctgtcttccg ccttggcggg gcgggcgggc ttgtggggcg tgaagctgcc ggaggtgtcg
     6781 ggttcctcca gcccgcggcg gatgatgagt tcggccatgg acgcgaatat ggagggccgc
     6841 gcccgccagc gcaaccggcc ccggcgcagc ggcccccgcc agccggcctg attgtgaccg
     6901 atttgtgcag gctgcaaaaa attgctcgca tgcgcagcaa cagcgcttgg caagcgcgga
     6961 tatttgtagc agcttgggtt taacagacag gatgaggttc atggccccaa gccctctctc
     7021 gtcattcgac gagatgatcg atgccgatgg taatatccgc cccgcctacg ccggctatcg
     7081 tgaatggttg gacgagcagg acggcaagtg gctgcagcgg caaagcaaac aggccgaaag
     7141 cttcttccgc aagacgggca ttactttcaa tgtctatggc gaggatgccg gggcggagcg
     7201 gctgatcccc ttcgacatgg tgccccgcat catcacccgc gatgaatgga gccggctggc
     7261 ggtggggatc gagcagcggg tgcgcgcgct caacgccttc ctccacgatc tttaccacaa
     7321 gcgcgagatc atcaaggccg gcaagctgcc cgagcggatg ctgaaggcca atgaggcctt
     7381 cctgccgcag atggccggtt tctcgccgcc gggcggggtc tacacccata tcgtcggcat
     7441 cgatctggtg cgcaccgggc ctgatgagtt catggtgctg gaagacaatg cccgcacccc
     7501 cagcggggtc tcctatatgc tggagaaccg cgagacgatg atggcgatgt tccccgagct
     7561 gttcacccgg gtgaaggtgc agccggtatc ggattatccg cgcagcctgg cccgttcgct
     7621 ggccgcctgc gcgccgccgg cctgcgagaa cgagcggccg gtggtggcgg tgttgacgcc
     7681 gggcatctac aattcggcct atttcgaaca tgccttcctg gccgaccaga tgggcgccga
     7741 gctggtggaa ggatcggacc tgcgcgtggt ggacgggcgc gtggcgatgc gcaccacccg
     7801 cggctggaag gcgatcgacg tgctttatcg ccgggtcgac gatgcctttc tcgatccgct
     7861 caccttcaac cccagcagcg tgctgggggt ggcgggcatc atggatgtct atcgcgctgg
     7921 cggcatcacc atcgccaatg cgccgggcac cggcatctcg gacgacaagg cgatctattc
     7981 cttcatgccc gagatcgtcg aattctacac aggcgagaag ccgatcctca agaacgtgcc
     8041 cacctggcgc tgcagcgagg aggacgcgct caaatatgtg ctcgacaatc tgaaggatct
     8101 cgtcgtcaag gaagtgcatg gttcgggcgg ctatggcatg ctgatcgggc ccaccagctc
     8161 gaaggccgag atcgccgcct tcgaaaagaa gctgcgcgcc aagcccgaca attacatcgc
     8221 ccagcccacc ctgtcgctgt ccaccgtgcc gatcttcacc aaggccgggc tggcgccgcg
     8281 tcatgtggat ctgcggccct tcgtgctggt ctcgcccggg ggcatcaaca tcacgcccgg
     8341 cggcctcacg cgggtggcgc tgaagaaggg ctcgctggtg gtcaattcca gccagggcgg
     8401 cggcacgaaa gacagctggg tgctggccga atgagtgcgc ggggcatggg ctgctgcggc
     8461 cgggcggcgt ggggagaggt gatctgatgc tgggccgttc cgcctggggc attttctgga
     8521 tgagccgcta tctggagcgg gcggagaaca ccgcccgcct gctggatgcg ggctttcgca
     8581 tcgcgctgac gcggggcaac agcaccgcca gcgacgaatg gcgcagcgtg ctggtgacgc
     8641 tggggctgga cgagcccttt gccgagcgcg gccgcgaggc caccggccct gccatcaccg
     8701 attatatcct gcgcgcgcag gacaacccca ccagcgtgct ggcgatgatg gagaatgcgc
     8761 gcaccaatgc ccgcatggtg cgcaccgcca tcacccgcga ggtgtgggag gccaccaacg
     8821 aaagctggat gatcctgaag gacgcgctgg ccaagcccgt gcgcgaggcc aatctgggcg
     8881 aggtgctgga tatgatccgc cgccaggcca cgctggtgcg cggggccatg gaaggcacga
     8941 tgctgcgcaa cgacatcctc gccttcgccc gcgccggcac ctatatcgag cgggccgaca
     9001 ataccgcgcg catcatcgat gtgaaatacc acgtgctgct gcccagcctg gcctgggtgg
     9061 gctcgcggct ggataatgcg cagtgggaga tggtgctgcg ctcggtggcg ggcgagcggg
     9121 cgttccgctg gctgcatgcg ggcgtgctcg atcccagggg aatcgccgag ttcctgattc
     9181 tcgacgcccg cttcccgcgc agcctggcct atgcctacaa gcagctggaa agcagcatgg
     9241 ccatcctgtc ggacgcctat ggcatcgaaa ccagcgccca tctggccgtg cgcgaggccg
     9301 tggcgcggct ggcggcgacg aatattgccg gaatcttcga tttcggtttg cacgaattca
     9361 ttactgaggc cattttcacc aatcgtcaga ttgccgaggc tatcgccgct gattaccgct
     9421 tcaccgaata acgccgaaag ggctctgtca tgcgcatcgc catcagccac accacgcgct
     9481 atcgcttcga cgagccggcc agctatgggc tgcagcgcct gcgcctgacg ccgagggact
     9541 gcacgctgca aagcgtggtg gactggcaca tggaaatcga gggcggggtg atggaagtgg
     9601 cctatgagga ccacaaccag aaccgggtgg cgctgatctc ctttggcggc ggcgcggggc
     9661 aagtggcgat cacctgttcg ggcgtggtgg aaacgcgcga tgcggcgggc gtggcggggc
     9721 cgcacaccgg tttcatgccg ctgtggcagc tgtgcgcgcc caccgaactg acgcgaccgg
     9781 ggccggcctt gtgcgcgctg gcggggcgct ttgccgcggc agagccggcc tgcggcacca
     9841 gcatgatcgg cgtgctgcac gatctggccc atgaggtggg cgaggcgatc gcttacgaca
     9901 agggccacac ccatgtgggc accaccgccg aggaggcgct ggtgctgggc aagggcgtgt
     9961 gccaggatca ggcccatgcc ttcatcgccg cggtgcgcct gctggggctg ccggcgcgct
    10021 atgtcagcgg ctatctgctg atggacgaga acgccgcgca ggaagccagc catggctggg
    10081 ccgaggcgca tgtgccgggg ctgggctggg tgggctttga tgtggcgaac gacatctgcc
    10141 cggacgagcg ctatgtgcgc ctggcggtgg gggccgatta tcgcgatgcc gcccctgtca
    10201 tcagccagac ccagggctgc ggcgaggcgc agctggaagt ggcggtggcg gtgcagcagc
    10261 gcatggcgat gcaataagcg cgaatccgta agggcttacc gcggggcgcg gggtggattc
    10321 gccaagggcg ggcggttctg ctagggcgcg ctggcgcccg gcggggcgat gatcggcaat
    10381 ctcgagggag cgtattcgtg acttattgcg tgggcatgat gctggacgca gggctggtgt
    10441 tgatgagcga cacccgcacc aattcgggcg tcgacaacat ctccaccttc cgcaagatgt
    10501 atagctggaa tgtgccgggc gagcggatcg tgacgctgat gaccagcggc aacctggcca
    10561 ccacccaggc cgttgtggcc aagctggaag agcgcaccaa ggcgccggcc gatcgcagca
    10621 acacgctgct gcacgcgccc acgatgtttt ccattgccac gctgatcggc aagctgctgc
    10681 gcgagacgat cgccgaccgc gacgaggaca atggcctgca ggcctcgggc accttttcag
    10741 ccacgctgat cctggcaggc cagatccgcg gcatgggcac gcatctctac ctgatctacc
    10801 ccgagggcaa tttcatcgag gccagtttcg acacgccctt cttccagatc ggcgagacga
    10861 aatatggccg gccgatcatc atccgcggct atgaccgcaa gatgagcttc gaggatgcgg
    10921 tgaagctgat gatggtcagc tttgattcga cgctgaaggc caatctgtcg gtgggcatgc
    10981 cgctggatct gatggtgatc gcgcgcgaca gtttcgagcc gctgcacgag gagcggatca
    11041 ccgcggcaga cccgtggtat cgcagcattt cggccagctg gagcgaggcc ctgcgcgccg
    11101 cgctcgatgc cctgcccgat ttccccttcc acgcgcgggg ggaatagggg cgtttgtggc
    11161 gcgcttgcgc ggcgccagcc ctctccccca cccggccacc ccca
//
LOCUS       DASRYE010000060         6629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_74340,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000060 DASRYE010000000
VERSION     DASRYE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74340"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /gene="glgX"
                     /locus_tag="VFF98_03270"
     CDS             <1..1708
                     /gene="glgX"
                     /locus_tag="VFF98_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZU62680.1"
                     /translation="TETRLPMTHGGDGWWRAELPGACKQLAYGFRAQGEWAPERGCWF
                     DPAKLLVDPYARVLDRRFTQSPMLAARGVETGAIVPRALLPGDMPQVPLAAPLFTPGG
                     LIYEVPVRGFTMLHPDVPEAQRGTVAALAHPAVIAHLRRLHVSAVELMPIIAWVDERH
                     LPPLGLGNAWGYNPVAMMALDPGLCPGGVAELRETVAALHAAGIGVLLDIVLNHTGES
                     DVQGGLLSLRGLDDSAYAKAGDGTLINDTGCGNTLDFAQPHVRALALAALRHFVAHCG
                     VDGFRFDLATVLARGPGFAADAPFFAELAADPWLASRVMIAEPWDIGPGGYQLGRFPA
                     HWLEWNDRWRDDVRRFWKGEAGLGELATRLAGSADIFGEQACRSVNFLAAHDGFTLAD
                     TLAYARRHNEANGEQNRDGHGQNHSWNHGAEGPSDDPAILAARAASARAMLGTLFAST
                     GTIMLTAGDEFGRSQQGNNNAYCQDNATSWVDWARRDLALEAHVVQLAATRARWQRQM
                     AQFPRSGAWQRLDGAPMRGADWEGQGSPGFAFLSHDAERPQGFCVDRAARQVALQLPP
                     GP"
     gene            1803..2516
                     /locus_tag="VFF98_03275"
     CDS             1803..2516
                     /locus_tag="VFF98_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZU62681.1"
                     /translation="MKKLLLATALMAFAPLAHAEDSAVRQPAEKPALHYLNPADVLPQ
                     RLYAQPPVAGSTYEKLELDFVRADIASASPERLAQAKWDEEHENPTAFNEVLGRDLTK
                     LPATWDLLITIQDEAYAVSSLAKDAFDRQRPFGLDHALPTCEKVDYAKTGRGYPSGHA
                     TVGYAVGWTLARLMPDIAPGILSRADEYAQSRSLCAAHFPSDTAASKAVAMLVAERLI
                     NDPRLADKIAAARKELSAQ"
     gene            complement(2714..5263)
                     /locus_tag="VFF98_03280"
     CDS             complement(2714..5263)
                     /locus_tag="VFF98_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62682.1"
                     /translation="MNRFSARQWLLASATSLTALGLAAPAFAADAAAANTADNAPITN
                     ADDIVVTATKVNAATPITASVHTFEPQAIVSRSIIEDSVPATADFTTIASLTPGASLV
                     DTGAGPGLGETKLVLRGFQDGQYNITYDGIPFGDSNDPTHHSTSYFPDGTYERIIVDR
                     GPGGATDLGQASYGGNVHLVSRELSDKFFVEGQGAYGSWNTYLLRGTVNSGRISKLGD
                     LKIIAVGEYKQSDTALTGSKGWYANSFVKLEKPLGPDAKLTVLSSYNQNFIHQADGAG
                     GATCTAGSIAGASVNGPYPETDGKNCAAGSQVATYGKQFALVDSSMLASSMWPGLRSD
                     WNWQRKVTDFEEARLQWNINDHISIDNKAYTYFYKNFTVSTDATTLKATGKANESFYG
                     VTPCALTYGGTQCWASATQAVTGTFLTPSSAAGGYGYTNDASLNGHMPGYTKLNQYRQ
                     IGDILEMTIKTSAGIGKLGFWYEHSRSHRYQYDYDFTAGAAAGGLSDGTFNFNIMNQN
                     YDYNVADKSYNTMSNGQYVPSYIKYDERTGWENYQVFGEFALKLLDDTLTLTPGVKVT
                     SYSRTINTPIASQSSRSSQFNHDNYKPVLPYFTANYLLKPNWSFYGQYAKGFLMPSLG
                     NSLESVVQNPTTGAITCPSSGCNLQPTKTKNYQLGTVYAGDRVNVDADVYYIQTSNST
                     SVDPSTNQAVQTAGPAEYQGVEGQISYVLMHGLTAIANGALMSSKDKTTHLWLSQSPN
                     YVATLGAVYGGSRFKLSYLHKFTGRQFADTAQLYRIAPYTKGTLSAAFKLGPAWLGVS
                     VDNVFNDTSVTKLGSSSSTTSPATSAPNTALPVAYYYQSPRSYQVNLKVRY"
     gene            complement(5432..6235)
                     /gene="cobA"
                     /locus_tag="VFF98_03285"
     CDS             complement(5432..6235)
                     /gene="cobA"
                     /locus_tag="VFF98_03285"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZU62683.1"
                     /translation="MTLAQRPHHVLPGTIWLVGAGPGDPELLTRKAERLIGEASVIFY
                     DALVGPGILELAGKGARLVYVGKRSGRHSRDQAGISALLVEAARAGERVVRLKGGDPA
                     IFGRAAEELAEARAAGIACHICPGITAASAAAASLGASLTLRGLARQLSFVTAHARAG
                     EELALDWQRLADAEATLAVYMGKAAAGTVAGQLLGAGMDGATPVAIVENASLPEERII
                     TTRLDLLAITARAALGDGPALLLIGNALRQNDIAPAQGRPATCNTDHPA"
     gene            complement(6232..>6629)
                     /locus_tag="VFF98_03290"
     CDS             complement(6232..>6629)
                     /locus_tag="VFF98_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZU62684.1"
                     /translation="AGDGARGMRRIALRGADGTLAAALYITRHGTLPAREWVAGQLGR
                     EGAGAMEWLAARPSTPQEEAGPLVCLCLGVGEKAIARAVDDIARAADDGACTVAAIGK
                     ATGAGTNCGSCRPAIARLLAAMPEKEAAQ"
BASE COUNT         1032 a         2115 c         2340 g         1142 t
ORIGIN      
        1 tacggaaacc cgcctgccaa tgacccatgg cggcgatggc tggtggcggg cggaattgcc
       61 gggcgcttgc aagcagcttg cctatggctt tcgcgcgcag ggggaatggg cgccggagcg
      121 cggctgctgg ttcgaccccg ccaaattgct ggtggaccca tacgcccgcg tgctggaccg
      181 ccgcttcacc caaagcccga tgctggccgc gcgcggggtg gaaacgggcg ccatcgtgcc
      241 gcgcgcgctg ctgccgggcg atatgccgca ggtgccgctg gcagcgccgc tgttcacgcc
      301 gggcgggctg atctacgaag tgccggtgcg cggcttcacc atgctgcacc ccgatgtgcc
      361 ggaagcccag cgcggcacgg tggcagcgct ggcccatccg gcggtgatcg cccatctgcg
      421 ccgcttgcat gtcagcgcgg tggagctgat gccgatcatc gcctgggtgg atgagcggca
      481 cttgccgccg ctgggcctcg gcaatgcctg gggctataat ccggtggcga tgatggcgct
      541 ggatccgggc ctgtgcccgg gcggggtggc cgaattgcgc gagacggtgg cggcgctcca
      601 tgccgcgggc atcggcgtcc tcctcgatat cgtgctgaac cacacgggcg agagcgatgt
      661 gcagggcggc ctcctttccc tgcgcgggct ggacgacagc gcctatgcca aggctggcga
      721 tggcacgctg atcaacgaca ccggctgcgg caacacgctg gattttgccc agccccatgt
      781 gcgggcgctg gcgctggcgg cgctgcggca ttttgtcgcc cattgcgggg tggatggctt
      841 ccgcttcgat ctggcaacgg tgctggcgcg cgggcccggc tttgccgcag acgcgccctt
      901 cttcgccgaa ctggccgccg acccctggct ggccagccgg gtgatgattg ccgagccctg
      961 ggatattggc ccgggcggct atcagctggg ccgtttccct gcccactggc tggaatggaa
     1021 cgaccgctgg cgcgatgatg tgcgccgctt ctggaagggc gaggcggggc tgggcgagct
     1081 ggccacgcgg ctggccggca gcgccgacat ctttggcgag caggcctgcc gcagcgtcaa
     1141 tttcctggcc gcccatgatg gcttcaccct ggccgacacg ctggcctatg cccggcgcca
     1201 caacgaggcc aatggcgagc agaaccgcga tggccatggg caaaaccaca gctggaacca
     1261 cggcgccgag gggccgagtg acgaccctgc catcctcgcc gcgcgggcgg ccagcgcgcg
     1321 ggcgatgctg ggcacgctgt ttgcctccac cggcaccatc atgctgaccg cgggtgacga
     1381 attcggccgc agccagcagg gcaacaataa cgcctactgc caggataatg ccaccagctg
     1441 ggtggattgg gcgcggcgcg atctggcgct ggaggcgcat gtggtgcagc tggccgccac
     1501 ccgcgcgcgc tggcagcggc agatggcgca gtttccgcgc agcggcgcat ggcagcggct
     1561 ggatggcgcg cccatgcgcg gcgcagactg ggaggggcag ggcagcccgg gctttgcctt
     1621 cctcagccac gatgccgaaa ggccgcaggg cttctgcgtg gaccgggcgg cgcggcaggt
     1681 ggccctgcag ctgccgccgg gcccatagcc gccgaatgac aggcgcctga caggccgggc
     1741 cggcacagtc atacaggcgt catgccagct acctaggcga ggggcaaagg aggactgccc
     1801 cgatgaaaaa gctgcttctt gccactgccc tcatggcctt tgcgccgctt gcccacgccg
     1861 aggacagcgc cgtgcgccag ccggccgaaa agcccgcgct gcactatctg aacccggccg
     1921 atgtgctgcc ccagcgcctt tatgcccagc cgccggtggc cggttccacc tacgagaagc
     1981 tggagctgga tttcgtgcgc gccgatatcg ccagcgccag cccggaacgc ctcgcccagg
     2041 cgaagtggga cgaggagcat gaaaacccca ccgccttcaa cgaagtgctg ggccgcgatc
     2101 tgaccaaact gccggccacc tgggatctgc tgatcaccat ccaggacgag gcctatgccg
     2161 tcagcagcct ggccaaggat gctttcgatc gccagcgccc cttcggtctc gatcatgccc
     2221 tgcccacctg cgagaaggtg gattacgcca agaccggccg cggctatccc agcggccatg
     2281 ccaccgtggg ctatgccgtg ggctggacgc tggcccggct gatgcccgac atcgctccgg
     2341 gcatcctcag ccgcgccgat gaatatgccc agagccgctc gctgtgcgct gcccatttcc
     2401 ccagcgacac cgccgccagc aaggcggtgg ccatgctggt ggccgagcgg ctgatcaacg
     2461 atccgcgcct ggccgataaa attgccgccg cccgcaagga actttccgcg cagtaaggcg
     2521 tttatatggc gcctctccct gtgggaggcg ctgccaagta gaagggccgg gcatgccgtg
     2581 tggcgcgccc ggcctttttg cgtctggcct atttgcgcct gcgggtgggc gccggccccc
     2641 caaagccaag ggccggaagg gattgctccc ttccggcccc cgggttgtgc agcctgtgcg
     2701 gcagcgctgg cgatcagtag cgaaccttga ggttcacctg atagctgcgc ggcgactggt
     2761 agtagtaggc caccggcagg gcggtgttcg gggccgaggt ggccggcgag gtggtgctcg
     2821 aggaagagcc cagcttggtc accgaagtgt cgttgaagac attgtccacg ctcacgccca
     2881 gccaggccgg gcccagcttg aaggcggccg acagcgtgcc cttggtgtag ggcgcgatcc
     2941 ggtagagctg ggcggtatcg gcgaactggc ggccggtgaa cttgtgcagg tagctcagct
     3001 tgaagcggct gccgccataa acggcgccca gcgttgccac atagttgggc gactggctca
     3061 gccacaggtg ggtggtcttg tccttcgagc tcatcagcgc gccattggcg atggcggtga
     3121 ggccatgcat cagcacatag ctgatctgcc cttccacgcc ctgatattca gccgggccag
     3181 cggtctgcac ggcctggttg gtcgagggat caaccgaggt cgagttcgag gtctggatgt
     3241 agtagacgtc ggcatcgacg ttcacccggt caccggcata gacggtgccc agctgatagt
     3301 tcttggtctt ggtgggctgc aggttacagc ccgaggacgg gcaggtgatg gcgcccgtgg
     3361 tggggttctg cacgacggat tccaggctgt tgcccagcga gggcatcagg aagcccttgg
     3421 catactggcc atagaacgac cagttcggct tcagcaggta gttcgccgtg aagtagggca
     3481 gaaccggctt gtaattgtcg tggttgaact ggctggaacg cgaggactgc gaagcgatcg
     3541 gcgtgttgat cgtgcgcgag tagctggtca ccttgacacc gggggtcagg gtcagcgtat
     3601 cgtccagcag cttcagcgcg aattcgccga acacctgata gttttcccag cccgtgcgct
     3661 cgtcatactt gatgtaggac ggaacatact ggccgttcga catggtgtta tacgacttgt
     3721 cggcgacgtt gtagtcgtag ttctggttca tgatgttgaa gttgaacgtg ccgtccgaca
     3781 gaccaccggc cgcagcgcca gcggtgaagt cgtagtcgta ctgatagcgg tgcgagcgcg
     3841 aatgctcgta ccagaagccc agcttgccga tgccggcgct ggtcttgatc gtcatctcga
     3901 ggatatcgcc gatctggcga tactggttca gcttggtgta gccgggcatg tggccgttga
     3961 gcgaggcatc gttggtgtag ccatagccgc cggcagccga gctgggcgtg aggaaggtgc
     4021 cggtgacggc ctgggtggcc gaggcccagc actgggtgcc gccataggtg agggcgcagg
     4081 gcgtcacgcc atagaaggat tcattggcct tgccggtggc cttcagcgtc gtcgcgtcgg
     4141 tcgagacggt gaagttcttg tagaagtagg tgtaggcctt gttgtcgatc gagatgtgat
     4201 cgttgatgtt ccactgcagg cgcgcttctt caaagtcggt caccttgcgc tgccagttcc
     4261 agtcggaacg caggcccggc cacatgctgc tggccagcat cgaggaatcg accagggcaa
     4321 actgcttgcc ataggtggcc acctgcgagc cggcggcgca gttcttgcca tcggtctcgg
     4381 ggtaggggcc gttcacgctg gcaccggcga tgctgccggc ggtgcaggtg gcgccgcccg
     4441 cgccatcggc ctggtggatg aagttctggt tatagctcga cagcacggtc agcttggcat
     4501 cggggcccag cggcttttcc agcttgacga aggagttggc ataccagccc ttcgagccgg
     4561 tcagcgccgt gtccgactgc ttgtattcgc ccaccgcgat gatcttcaga tcgcccagct
     4621 tgctgatgcg gcccgagttg acggtgccgc gcagcagata ggtgttccag ctgccatagg
     4681 cgccctggcc ctcgacgaag aacttgtccg acagttcgcg gctgacgagg tggacgttgc
     4741 cgccatagct ggcctggccc agatcggtgg cgccgccggg gccgcgatcg acgatgatgc
     4801 gctcgtaggt gccatcgggg aaataggagg tggagtggtg ggtgggatcg ttggaatcgc
     4861 cgaaggggat gccatcgtag gtgatgttgt actggccatc ctggaagccg cgcagcacca
     4921 gcttggtttc acccaggccc gggccggcgc cggtgtccac caggctggcg cccggcgtca
     4981 gcgaggcgat ggtggtgaag tcggccgtgg ccggcaccga atcctcgatg atcgagcgcg
     5041 acacgatcgc ctgcggctcg aaggtgtgca ccgaggcggt gatcggcgtg gccgcgttca
     5101 ccttggtggc ggtcacgacg atgtcgtcgg cgttggtgat gggtgcgttg tcggccgtgt
     5161 tggcagcagc ggcatcggca gcaaaggccg gcgcggcaag gccaagagcg gtgagcgagg
     5221 tggcgctggc cagcagccac tggcgcgcgg aaaagcggtt catgaaagcc ccctgaatgg
     5281 aacggatggt ggttccgtga gggcgccctg acaggccttg gtgacgttcc ggcgacgctt
     5341 tgatgaccgt cagggggcag aatgatgact ttagaaagac acttgtccgc cgtgtttgcg
     5401 gaaacctttg cgaaaacctt ggggttttac ctcaggcggg gtggtctgtg ttgcaggtgg
     5461 cggggcgccc ttgcgcgggg gcgatgtcgt tctggcgcaa agcgttgcca atcagcaaca
     5521 gggccgggcc atcgcccagc gcggcgcggg cggtgatcgc cagcagatcg agccgggtgg
     5581 tgatgattcg ctcttccggc aggctggcgt tctcaacaat cgccaccggc gtggccccat
     5641 ccatgcccgc gcccagcaat tgcccggcca ccgtgcccgc cgccgccttg cccatataga
     5701 cggccagcgt ggcctcggca tcggccaggc gctgccagtc cagcgccagt tcctcgccgg
     5761 cgcgggcatg ggcggtgaca aagctgagct ggcgcgccag cccgcgcagg gtgagcgagg
     5821 cgcccaggct ggccgccgcc gcgctggccg cggtgatgcc cgggcagatg tggcaggcga
     5881 tgccggccgc ccgcgcctcg gccagctctt ctgccgcgcg gccgaagatg gcggggtcgc
     5941 cgcccttcag ccgcaccacc cgctcgcccg cgcgggccgc ttccaccagc agcgcgctga
     6001 tgccggcctg atccctggaa tggcggccgg aacgcttgcc gacatagacc agccgcgccc
     6061 ccttgccggc cagctccaga atgccggggc ccaccagcgc gtcgtagaag atcacgctgg
     6121 cctcgccgat cagcctttcg gccttgcggg tcagcagttc ggggtctccg ggcccggcgc
     6181 ccaccagcca gatggtgccg ggcagaacat ggtgcgggcg ctgggcaagg gtcattgtgc
     6241 tgcttccttc tcgggcatgg cggccagcag gcgagcgatc gccggccggc acgagccgca
     6301 attggtgccg gcgcccgtgg ccttgccaat ggccgccacg gtgcaggcgc catcatcggc
     6361 cgcgcgggca atatcgtcca ccgcgcgggc aatcgccttc tcgcccacgc ccaggcacag
     6421 gcacaccagc ggccctgcct cttcttgcgg cgtactgggc cgcgcggcaa gccattccat
     6481 ggcgccagcg ccttcgcggc ccaattggcc ggccacccat tcgcgcgcgg gcagggtgcc
     6541 gtggcgggtg atataaagcg cggctgccag cgtgccatcg gcgccgcgca gggcaatgcg
     6601 gcgcatgccg cgcgcgccat cgcccgctt
//
LOCUS       DASRYE010000061        10445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_127052,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000061 DASRYE010000000
VERSION     DASRYE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127052"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1725)
                     /locus_tag="VFF98_03295"
     CDS             complement(112..1725)
                     /locus_tag="VFF98_03295"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZU62685.1"
                     /translation="MSQEIDVAIVGFGPAGEVLASTLGEAGLRVLVFERWPQPYPLPR
                     LTTLDGECCRVVQATSADVDRGFSESCVQDAAHFVDAEGQPLMRVLYPGQIGGWPARV
                     SIFQPDFERGIADKVAAMANVEVRRGWEVQAIAQDGEGVTLRAAPFDAAVMQGGGQAA
                     GQESETFRARYVVGTDGARSFVRQALGIAVKDFDLHERWLNFDAEIKRPLPAHFGKLA
                     IFMDPARPHMYMPIGERYLRLEFRVMEGESDEQVTAPEIAWDFLTRQHGLGPDDVSIM
                     RQVVYHYHTRLAQDWKVGRVFIAGDAAHTMPPYMGQGGCAAIRDGRNLGWKLVEVLSG
                     RSGEALLDTYQEEREPHLTTLLLASDRLSRMVNTVDPQEAEARNAGMRTGGDRRPPDL
                     PGLAAGVLHRLAADRLGPAAGVMTPQGIIRRGLRWARGDDLLGSGFQIWALGDVAAHL
                     APQTRAWLEARQVALAVFGDPAAAHAAEDTEGVYTAFLRQHGVELLIVRPDFFAFGGG
                     ALTEADSLVADLARQLHAPIGAALARALA"
     gene            1880..2842
                     /locus_tag="VFF98_03300"
     CDS             1880..2842
                     /locus_tag="VFF98_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62686.1"
                     /translation="MTNDTGKIAETSRIDLNLLLIFDVVMIERHVTRASIRLGLTQSA
                     VSNALNRLRTLFDDMLFVKAARGVEPTQRALVLWPEIHAAIAQLQRSVRPGGFDPMTS
                     EIDFRMSMVDLSAALLTPHLYGRMRAESPTVSLSFIPHVPELTAERLVRGEVDFAISI
                     EPPRMAVLETVPLWSERYVVAARRDHPVIGRPVDMEVLCTHPQLLVNLSGAASFLSPI
                     DQYFQERGLRRPIKLVVNQFLVATTMLRESDLIAILPERLVGDAFRQNWLDYQPLPIS
                     LAEAALHLIWHKRNNGLQAMSWLKAKILEATAALNEESRAMVRR"
     gene            complement(2855..4093)
                     /locus_tag="VFF98_03305"
     CDS             complement(2855..4093)
                     /locus_tag="VFF98_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62687.1"
                     /translation="MRPGGNLVHLRLSAMMFLQYFVWGAWFVPLGTYMAAARHFTAGD
                     IGMAFGMQGVGAIIAPLAVGVIADRFMASQKVFGLLHLAAAVAMYLLAGAAGARPFLA
                     MVLVYFVLYMPTLPIANAISFGALRNVEQEFPAIRVFGTIGWICSGLAIGLIPGAATS
                     GLPMGISAIGSLVLGVYAFSLPDVPPRGRAQGSGVMALLGLDILAGMKSRSFWVLIAG
                     STLLVIPLSFYYAFTNPFLSALHARLPLFSLSLEPTGMMVFGQLSETLFIVLLPVLLR
                     HGGIRRIMIAGMLAWVARYLCFAYGFTAAGPITGLLMIGILLHGVCYDFFFVAGQIHV
                     DQAFDPSQRARAQSFLALMTLGVGQVAGSNLAGRIVEAASSGGVVDWRVVWLLPAALA
                     LVVALGFALLFRAEQGKAAG"
     gene            complement(4120..4950)
                     /locus_tag="VFF98_03310"
     CDS             complement(4120..4950)
                     /locus_tag="VFF98_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU62688.1"
                     /translation="MLKLGTTLFSFQLEYVQNRWSLEDCMELAAYTGGDRGVELVGPM
                     HHRSWPDLTPEDERRLKSAFERWELVPTHYGAYTDMSYYHALDARFDFQIRQFKTARQ
                     LGYRIVRIQSPGVDPILERLVGAAEKMGLKLAIEIHVPKMFENPEFQALLAQVRQISS
                     PSLGFIIDTGIFEDVTGVRRFIGEAVDAQPFAPSRPETMLEFMPLIMGLHGKFHRMDK
                     GQIPEVPFERIVSVLAQGRFDGWMSTEFEGGQLGAYSNSFAVVQAHHRLMKTFIARYY
                     "
     gene            complement(4966..5631)
                     /locus_tag="VFF98_03315"
     CDS             complement(4966..5631)
                     /locus_tag="VFF98_03315"
                     /inference="COORDINATES: protein motif:HMM:NF040396.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6379 domain-containing protein"
                     /protein_id="HZU62689.1"
                     /translation="MAMKTQEEQERGRGLMDRRAFGGSLALGAGLMAAPIGLGAQTPL
                     APQAAPGRVAARFPTEYMPAGRAIVRHDGIAGSFTGAQHALTPLINPDHGLRAAICNG
                     EAGLTFQIKETGYRGIPLFAVRGVEVEIDGAAVAQEALTFTLDNHAYRIADLPAQKTV
                     QWWLFDWATIFVPRAGGFAAGSHRLKVRFTYVSIYGAKPTDLAVLSAEEVLPLQPLTP
                     FEA"
     gene            complement(5779..7974)
                     /locus_tag="VFF98_03320"
     CDS             complement(5779..7974)
                     /locus_tag="VFF98_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62690.1"
                     /translation="MLRATTAVLVVCAVPSFAQAQNAASGGADKGASVPEIIVTATRQ
                     ESSAQKTGVALNVYSGETLQQQGVRSVADLSKIDPSLTFNAATGEPYLAIRGVSSADT
                     TEIGDPAVPVARDGFFSNRTFTIDTSLYDLQRIEVLKGPQGTLFGRNAAGGLVSIVTA
                     EPDRKLGGYLNGTLGDYSQRQIEGALNLPVSDRYRFRLAGLYGAHDGYRHLTGIGGRG
                     DDQDVKSLRGKFAFEPVDHLKGLIAVQYDKVGGVGDVFVPTAYGQVPHFTSASSKTFG
                     NFSPTAMDIEAFRLNWRLAYDNLPFGTTLTYLGGYDDSRYHHVLDASTASAAGALTAG
                     SIQQYFQNEAPRTWNHEVRLSSAGDRALTWQLGFFNFQESNRIDSHILYESGLVAGQN
                     LIHFNYGVTTISNAVFGQASYRILDNLKLSAGARWTNDEKTRTGNSTLDITVASGGFA
                     TLPGGAHIVTVTDGYGHLSASRASFHAGLEWQASPHHLVYAKFDDGYKPGGFNSIGAG
                     ATIPYGAETVKAFELGTKNDFFGRHLIVNLDYFHQNFDNYQANQYSNAVVGGGVLVEN
                     TGNAGIDGIEGELRYQSAAFGRLSVNATWLHTRFDNAIAADDQSVNRQIGGNRLPDSP
                     AIVVAASYEHDFDLGFGTLTGHGDVKYSARYYYSIFNFADLASPATTTANLSVSFAPP
                     SKSWSVQAYVRNVTDAVVISSAVRNAISGFNQYEFAAPRTFGVMASYKF"
     gene            complement(8268..9950)
                     /locus_tag="VFF98_03325"
     CDS             complement(8268..9950)
                     /locus_tag="VFF98_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZU62691.1"
                     /translation="MADFDAIVIGSGITGGWAAKELTERGLKVLMIERGPLIEHQSGY
                     VNEMKAPWEMPFHGQGDANLYKREYFIQSKKTMFFNEWNQQHWANDKENPYSEAPGTK
                     FEWYRGYQLGGKSLTWGRQCYRWSDTDFGANKADGHGIDWPIRYADVAPWYDHVEDFI
                     GVSGSAEGLDILPDGHFLPPMELNAGERHVKALVEAQYPGRKLIIGRSANITRPVKDR
                     APCQYRGICNRGCSYGAYFSTQSSTLPAARATGRLTLMTDTVVERIEHDPATGKATGV
                     RTIGTKDRKRGQFTARMIFCNAGTINTVGVLLRSTSERFPHGLGNSSGTLGKYLMDHA
                     TAMSAMALINGVDDRAYYGNRPNNIIMPRYRNIGADREDFLRGFSYQGGAMRRGWKRG
                     GGTAGLGAGLKDQLKGPGGWMMVFGAFAECLPNAANRISLHPTKTDSQGLAQVEIAFS
                     YGPNERAALRKAESDAREMLEAAGATILATTSEPDAGGSAIHEMGGARMGADPAQSVV
                     NAFNQCHDVPNVFVTDGACMSSTACQNPSLTYMALTARAAAAAVSKLQEGAV"
     gene            complement(9950..>10445)
                     /locus_tag="VFF98_03330"
     CDS             complement(9950..>10445)
                     /locus_tag="VFF98_03330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZU62692.1"
                     /translation="GFHAWLASGPGAASPDFLRQIANQVIPRSRTPGAGEVPEVLAFV
                     NRAMPAGLYGGSETTLGALERALDGMVASSRFVKATSADQVKVLTAIDKDAFAHRAEA
                     GQEAAVHALWRCVKEAIVVGYYTSETGGAHELAYELVPGRFDPDVPLGSVPYLSNYWL
                     ENVF"
BASE COUNT         1941 a         3420 c         3316 g         1768 t
ORIGIN      
        1 ccagatcggg cgtgtgatag ccgaaatggc cgagcctctc gatgcggaac ggcctgtcga
       61 gttcgaggcc gccgacaaca taggtcatgg caaaaatcct gtcttgaagg ttcaggccag
      121 ggcccgggca agggctgccc cgatgggggc gtgcaactgg cgagcaagat cggcgacgag
      181 gctatcggcc tcggtcaggg cgccgccgcc aaaggcgaag aaatccgggc gaacgatgag
      241 cagttcgaca ccgtgctggc gcaggaaggc ggtgtagacg ccttctgtat cctcggcggc
      301 atgggccgcg gccggatcgc cgaaaacggc cagcgccacc tggcgcgcct ccagccatgc
      361 ccgcgtttgc ggcgccagat gcgccgcaac atcgcccagc gcccagatct ggaagccgct
      421 gcccagcaga tcatcgccgc gcgcccagcg caggccgcgc cggatgatgc cttgcggggt
      481 catcacgccg gccgccgggc ccagcctgtc ggccgccagg cggtgcagca cgccggcggc
      541 cagccccggc agatcgggcg ggcggcgatc gccccctgtc cgcatgccgg cattgcgcgc
      601 ctcggcctcc tgcggatcga cggtgttgac catgcgcgag aggcgatcgg aggccagcag
      661 cagggtggta aggtgcggct cgcgctcttc ctgataggtg tcgagcaggg cctcgccgct
      721 gcggcccgac agcacctcca ccagcttcca gcccagattg cgcccgtcgc ggatcgccgc
      781 gcagcccccc tggcccatgt agggcggcat ggtgtgcgcc gcatcgccgg cgatgaacac
      841 ccggcccacc ttccagtctt gcgccagacg ggtgtggtag tggtagacga cctgccgcat
      901 gatgctcaca tcatccgggc ccaggccatg ctggcgggtc aggaagtccc aggcgatctc
      961 gggcgcggtc acctgctcgt cgctctcgcc ttccatcacg cggaattcga ggcgcaggta
     1021 gcgctcgccg atcggcatgt acatgtgcgg gcgcgccgga tccatgaaga tggccagctt
     1081 gccgaaatgc gcaggcagcg ggcgcttgat ctcggcatcg aagttcagcc agcgctcgtg
     1141 caggtcgaaa tccttcaccg cgatgcccag cgcctggcgc acgaagctgc gcgcgccatc
     1201 ggtgcccacc acatagcggg cgcggaaggt ttcgctctcc tgcccggcgg cctgcccgcc
     1261 gccctgcatc acggccgcat cgaagggcgc cgcgcgcagg gtcacgcctt cgccatcctg
     1321 cgcgatggcc tgcacttccc agccgcgccg aacctcgaca ttggccatcg ccgccacctt
     1381 gtcggcaatg ccgcgctcga aatcgggctg gaagatcgag acgcgcgccg gccagccgcc
     1441 gatctgcccg ggatagagca cgcgcatcag cggctggccc tcggcatcga cgaaatgggc
     1501 ggcatcctgc acgcagcttt cgctgaaacc gcgatcgaca tcggcgctgg tggcctgcac
     1561 cacccggcag cattcgccat ccagcgtggt caggcgcggc agcggatagg gctgcggcca
     1621 gcgctcgaac accagcaccc gcaaccccgc ctcgcccaag gtcgaggcca gcacttcacc
     1681 cgccggcccg aacccaacga tcgccacatc gatttcctgc gacacctgcc cactcccgtt
     1741 tcatttcagc ttgtggggtc atctttatgg gtgccgaaat cattgaacaa cgcagtcacc
     1801 tatattacaa ttatcacgca aaatgatgat gccggcgatc gatccgatgc gcccggcacg
     1861 ccgccatggg agagaaacgg tgaccaacga caccggcaag atcgcggaaa cgtcacgcat
     1921 cgatctgaac ctgctgctta ttttcgatgt cgtcatgatc gagcggcatg tcacgcgggc
     1981 ctccatccgc ctgggcctga cgcaatcggc ggtcagcaat gcgctcaatc gcctgcgcac
     2041 cctgttcgac gacatgctgt tcgtgaaggc ggcccggggg gtggagccga cccagcgcgc
     2101 gctggtgctg tggcccgaga ttcacgccgc catcgcgcag ctgcaaaggt ctgtccgccc
     2161 cggcggcttt gatcccatga cgagcgagat cgatttcagg atgtcgatgg tcgatctgtc
     2221 cgccgccctg ctcaccccgc acctctatgg caggatgcgg gcggaatcgc ccaccgtcag
     2281 cctgtcgttc attccccatg tgcccgaact gaccgccgag cgcctggtgc gcggcgaggt
     2341 tgattttgcc atcagcatcg agccgccgcg catggccgtg ctggaaaccg tgccgctgtg
     2401 gtcggagcgc tatgtcgtgg ccgcaaggcg cgatcacccg gtgatcggcc ggcctgtcga
     2461 catggaagtg ctgtgcacgc acccgcaatt gctggtcaac ctctcgggcg cggcctcctt
     2521 cctctcgccg atcgaccagt attttcagga aagaggcctc aggcggccga tcaagctggt
     2581 ggtgaaccag tttctggtgg ccaccaccat gctgcgcgaa tccgatctga tcgccatcct
     2641 gccggaacgg ctggtgggcg atgcctttcg ccagaactgg ctcgattacc agccgctgcc
     2701 catctcgctg gcagaagccg cgctgcatct gatctggcac aagcgcaaca acgggctgca
     2761 ggccatgagc tggctgaagg ccaagatcct ggaggcgaca gcggcgctga acgaggaatc
     2821 acgcgcgatg gtgcggcgct gaaaggcgcg gggctcagcc ggcagctttg ccctgttccg
     2881 cgcggaaaag cagggcaaag cccagcgcca caacaagcgc gagggcggcc ggaagcagcc
     2941 agacgacccg ccagtccacc accccgccag aagatgccgc ctcaacgatc cggcccgcca
     3001 gattggaccc tgcgacctgg cccacgccca gcgtcatcag cgccagaaag ctttgcgcgc
     3061 gggcgcgctg gctggggtcg aaggcctgat cgacatggat ctgcccggcg acgaaaaaga
     3121 aatcataaca cacgccatgc agcaggatgc cgatcatcag caggccggtg atcggcccgg
     3181 cggcggtgaa gccataggca aagcacagat agcgggcgac ccaggccagc atcccggcga
     3241 tcatgatccg cctgatcccg ccatggcgca gcaaaacagg cagcagcacg atgaaaagcg
     3301 tttccgaaag ctggccgaag accatcatgc cggtcggctc gaggctgagg gaaaacaggg
     3361 ggagcctggc gtgcagggcg ctcaggaacg ggttggtgaa ggcgtaatag aaggaaaggg
     3421 ggatgaccag caaggtcgag ccggcgatca gcacccagaa cgagcggctt ttcatcccgg
     3481 ccagaatatc aaggcccagc aacgccatca cgcccgatcc ttgcgccctg cccctgggcg
     3541 gcacatcggg caggctgaag gcataaacgc ccagcaccag cgagccgatc gccgatatcc
     3601 ccatcggcag cccgctcgtt gccgcgccgg ggatcaggcc gatggccagg ccagagcaga
     3661 tccagccgat cgtgccgaac acgcggatgg cgggaaattc ctgctcgaca ttgcgcagcg
     3721 cgccaaagct gatggcattg gcaatgggca aggtgggcat gtagagcacg aaatagacca
     3781 gcaccatcgc cagaaagggc cgcgccccgg ccgcccccgc cagcagatac atcgccaccg
     3841 cggcggccag atgcagcagg ccgaaaacct tttgcgacgc catgaaccgg tcggcgatca
     3901 cccccacggc cagcggcgcg atgatagcgc ccaccccctg catgccaaac gccatgccaa
     3961 tatcgccggc cgtgaaatgg cgcgcggccg ccatataggt gcccagcggc acgaaccatg
     4021 cgccccacac gaaatattgc aggaacatca tcgccgacag gcgcagatgc acgaggtttc
     4081 cgccagggcg caagatcggg gcttccgtca gacagagggt cagtaatacc gggcgatgaa
     4141 cgttttcatc agccggtgat gggcctgcac cacggcaaag gaattgctgt aggcgcccag
     4201 ctgcccgccc tcgaactcgg tggacatcca gccgtcgaag cggccctgcg ccagcacgct
     4261 gacgatccgc tcgaacggca cctcggggat ctggcctttg tccatgcggt ggaacttgcc
     4321 gtgcaggccc atgatcagcg gcatgaactc cagcatcgtc tcgggcctgg agggcgcaaa
     4381 gggctgcgca tcgaccgcct cgccgatgaa ccggcgcacg cccgtcacat cctcgaagat
     4441 gccggtatcg atgatgaaac ccagcgaggg cgaggaaatc tgccgcacct gcgccagcaa
     4501 ggcctgaaat tcggggttct cgaacatctt gggcacatgg atctcgatgg ccagcttcag
     4561 ccccatcttc tctgccgcgc ccaccagccg ttcgagaatg ggatccacgc ccggcgactg
     4621 gatccgcacg atgcgatagc ccagctgcct ggcggttttg aactgcctga tctggaaatc
     4681 gaagcgcgca tccagcgcat ggtaatacga catgtcggtg taggcgccat aatgggtggg
     4741 caccagttcc caccgctcga aggcactttt caggcggcgc tcgtcctcgg gcgtcagatc
     4801 cggccaggaa cggtgatgca tggggcccac cagctccaca ccgcgatcgc cgccggtata
     4861 ggcggccagt tccatgcaat cctccagaga ccagcgattt tgcacatatt ccagctggaa
     4921 gctgaacagg gtggtgccaa gtttcaacat gtggggctcc cgcgctcagg cttcgaaggg
     4981 ggtaaggggc tgcagcggca gcacttcctc ggcgctcagc accgcaagat cggtgggctt
     5041 tgcgccatag atgctcacat aggtgaagcg caccttcagc cggtggctgc ccgcagcaaa
     5101 gcccccggcc ctgggcacga agatcgtcgc ccagtcgaac agccaccatt gcaccgtctt
     5161 ctgggcgggc aggtcggcga tcctgtaggc gtggttgtcg agcgtgaagg tcagcgcttc
     5221 ctgcgccacg gcagccccgt cgatctcgac ctcgacgccg cgcaccgcga aaagcggaat
     5281 gccgcgatag ccggtttcct tgatctggaa ggtcagcccg gcctcgccgt tgcagatcgc
     5341 cgcgcgcagg ccatgatcgg gattgatcag cggggtgagc gcatgctggg cgcccgtgaa
     5401 cgagccggca atgccatcat gccgcacgat agcgcggccg gccggcatgt attcggtggg
     5461 gaaacgggcg gccacccggc ccggcgccgc ttgcggggcc agcggcgttt gcgcgccaag
     5521 gccgatgggg gccgccatca ggccggcgcc aagggccagg ctgccgccaa acgcgcggcg
     5581 atccatcagg cccctgccgc gttcctgttc ttcctgtgtt ttcatggcca tgggcgtcat
     5641 cctctgcatg cttttgcggc taaccataaa cggcgggcat ggcgcggcaa tcgcgcctgt
     5701 gcggataatg gtcatcactt gtggtgataa ggggcgccgc cgccgaacag ggcgcccctc
     5761 ctcctgcctg cccttcgatc agaacttgta ggaggccatc acgccaaagg tgcgcggcgc
     5821 ggcaaattca tactggttga agccggagat cgcattgcgc accgcgctgc tgatgacgac
     5881 agcatcggtt acattgcgca cataggcctg caccgaccag cttttcgacg gcggggcgaa
     5941 cgacacggac aggttggccg tggttgtggc gggcgaggca agatcggcga aattgaagat
     6001 cgaatagtaa tatcgggccg aatacttgac atcgccatgg ccagtgagcg tgccaaaacc
     6061 cagatcgaaa tcatgctcgt aggaagcggc cacgacgatg gcgggcgaat ccggcaggcg
     6121 gttgccgccg atctggcggt tcacgctctg atcgtcggcg gcgatggcat tgtcgaaccg
     6181 ggtgtgcagc caggtggcat tcacgctcag ccggccgaag gcagcgctct ggtagcgcag
     6241 ttcgccctcg atgccatcga tcccggcatt gccggtgttc tcgacgagaa cgccgccgcc
     6301 caccaccgcg ttcgaatatt ggttggcctg gtaattgtcg aagttctggt ggaaataatc
     6361 gaggttgacg atgagatggc ggccgaagaa atcattcttc gtgcccagct cgaacgcctt
     6421 cacggtttcc gcgccataag gaatggtcgc gcccgcgccg atcgagttga agccgcccgg
     6481 cttgtagcca tcgtcgaact tggcatagac caaatgatgg gggcttgcct gccactccag
     6541 cccggcatgg aagctggccc tggaggcgct gagatggcca tagccatcgg ttacggtgac
     6601 gatgtgggcg ccgcccggca acgtggcaaa accgcccgag gccaccgtga tatccagcgt
     6661 ggaattgccg gtgcgggtct tctcgtcatt ggtccagcgg gcgccggcag acagtttgag
     6721 attgtcgaga atgcggtagc tggcctggcc gaacacggcg ttggagatgg tcgtcacgcc
     6781 atagttgaag tggatcaggt tctggccggc aaccaggccg gattcataga ggatgtggct
     6841 gtcgatccgg ttgctttcct ggaagttgaa gaagcccaat tgccaggtca gcgcccgatc
     6901 acccgccgag gacaggcgca cttcatggtt ccaggtgcgg ggcgcctcgt tctggaaata
     6961 ctgctggatc gagccggccg tcaacgcccc cgcggcgctg gccgtcgagg catccagaac
     7021 atggtgatag cgcgaatcgt catagccgcc cagataggtc agcgtcgtgc cgaaaggcag
     7081 gttgtcatag gccagccgcc agttcaggcg gaaggcctcg atatccatcg cggtgggcga
     7141 gaaattcccg aaggtcttgc tcgatgcgct ggtgaaatgc ggcacctgcc cataagcggt
     7201 gggcacgaac acatcgccca ccccgccaac cttgtcatat tgcaccgcga tcaggccctt
     7261 gaggtgatcc accggctcga aagcgaactt gccacgcagc gatttgacgt cctgatcatc
     7321 gccccgcccg ccaatgccgg tgaggtggcg atagccatca tgcgcgccat aaaggccggc
     7381 caggcggaag cgatagcgat ccgacaccgg caggttgagc gcgccctcga tctggcgctg
     7441 gctgtaatcg cccagcgtgc cattgagata gccacccagc ttgcgatcag gctcggcagt
     7501 gacgatgctg accaggccgc cggccgcatt gcggccaaac agcgtgccct gcggcccctt
     7561 gagcacttcg atgcgctgca gatcgtagag cgatgtgtcg atggtgaaag tgcggttgct
     7621 gaagaagcca tcgcgcgcca ctggcaccgc gggatcgccg atctcggtcg tatcggccga
     7681 ggagacgccg cgaatggcca gatagggctc gccagtggcg gcgttgaagg tcaggctcgg
     7741 atcgatcttc gacagatccg ccaccgatct cacgccctgc tgctgcagcg tttcgccgct
     7801 gtagacgttc agcgcaacgc cggtcttctg cgcggaggat tcctggcgcg ttgccgtgac
     7861 gatgatttcg ggaacagaag cgcctttatc cgcgccgccc gaagcggcat tttgcgcctg
     7921 cgcgaatgac gggacagcac agaccaccag aacagcagtc gtcgcacgga gcattgccga
     7981 tgatttcatt gatgaaccct tccccatcat ttttgattat ttcgtgaaat tgcgccacat
     8041 ctcttgggag tgactgcatc tgtgcatcac gctgcgcagc aacctcgtac acgcggcgcg
     8101 atcatattct caataatgcc gcggatataa tcgtcatcat tacgagtgat gatctgacgg
     8161 gagagcgccg gcgaaccgcc cggccacaac ggcaaagggc ccggcgtttc ccccgggccc
     8221 ttccttgtgc ctcttgcgtt acgcaggcag gcgcccgcgc gccaagctca gacagcgcct
     8281 tcctgcagct tcgagacagc cgccgccgcc gcccgggccg tcagcgccat ataggtcagg
     8341 gaggggttct ggcaggcggt cgagctcatg caggccccgt cggtgacaaa gacgttcggc
     8401 acgtcgtggc actggttgaa ggcgttcacg accgattggg ccgggtctgc gcccatgcgc
     8461 gcgccgccca tctcgtgaat ggccgagccg cccgcatccg gctcggatgt ggtggcaagg
     8521 atcgtggcgc cggccgcctc gagcatctcg cgcgcatcgc tctcggcctt gcgcagcgcg
     8581 gcgcgctcgt tggggccata gctgaaggcg atctccacct gggccagccc ttgcgaatcc
     8641 gtcttcgtcg gatgcagcga gatccggttg gccgcattgg gcagacattc ggcaaaggcg
     8701 ccaaacacca tcatccagcc gccgggcccc ttcagctgat ccttcagccc tgcccccagc
     8761 cccgccgtgc cgccgccccg tttccagccg cggcgcatcg cgcccccctg gtaggagaag
     8821 ccgcgcagga agtcctcgcg atcagcgccg atgttgcgat agcgcggcat gatgatgttg
     8881 ttgggccggt tgccatagta ggcccggtcg tcgacgccgt tgatcagcgc catcgccgac
     8941 atcgccgtgg catgatccat gagatatttg cccagcgtgc cgctgctgtt gcccagccca
     9001 tgcgggaagc gctccgaggt ggagcgcagc agcacgccca cggtgttgat cgtgccggcg
     9061 ttgcagaaga tcatccgcgc ggtgaactgg ccgcgcttcc tgtctttcgt gccgatggtg
     9121 cgcacgccgg tggccttgcc ggtggccgga tcatgctcga tccgctcgac caccgtgtcc
     9181 gtcatcaggg tcaggcgccc ggttgcgcgg gcggccggca gggtggagga ttgcgtggag
     9241 aaataggcgc catacgagca gccgcggttg cagatccctc gatactggca cggcgcgcga
     9301 tccttcaccg gccgcgtgat gttggcggaa cggccgataa tcagcttgcg ccccggatat
     9361 tgcgcctcga ccagcgcctt gacgtggcgc tcgccggcgt tgagctccat cggcggcagg
     9421 aaatgcccgt ccggcaggat gtcgagcccc tcggcggaac cggaaacgcc gatgaaatcc
     9481 tccacatgat cataccaggg cgccacatcg gcatagcgga tcggccagtc tatgccgtgg
     9541 ccatcggcct tgttggcgcc aaaatcggtg tcggaccagc gatagcactg gcgcccccag
     9601 gtgagcgatt tgccgcccag ctgatagccg cgataccatt cgaatttggt gcccggcgct
     9661 tccgaatagg ggttttcctt gtcgttggcc cagtgctgct ggttccattc attgaagaac
     9721 atcgtcttct tgctctgaat gaaatattcg cgcttgtaga gattggcatc gccctgcccg
     9781 tggaagggca tttcccaggg cgccttcatc tcgttcacat agccgctctg atgctcgatc
     9841 agcggcccgc gctcgatcat cagcaccttg agcccgcgct cggtgagttc cttcgcggcc
     9901 cagccaccag tgatgcccga accgattaca attgcgtcaa aatcagccat cagaacacat
     9961 tctctagcca gtagttcgac agatagggca cgcttccgag cggcacatcg ggatcgaaac
    10021 ggccgggcac cagctcatag gccagctcgt gggcgccgcc ggtctcgctg gtgtaatagc
    10081 ctaccacgat cgcctccttc acgcagcgcc acagggcatg gaccgcggct tcctgccccg
    10141 cttcggcgcg gtgtgcgaaa gcatccttgt cgatcgccgt gagcactttc acctgatcgg
    10201 cgcttgtggc ttttacgaaa cgcgacgaag cgaccatgcc atcaagggcg cgttcaaggg
    10261 cgcccagcgt ggtttcgctg ccgccataga ggccggccgg cattgcccgg ttcacaaagg
    10321 ccagcacctc gggcacctcg ccagcgcccg gcgtccggga tcgggggatc acctgattgg
    10381 cgatctggcg caggaaatcc ggcgaggccg ccccgggccc gctggccagc caggcatgga
    10441 agccg
//
LOCUS       DASRYE010000062        20189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_233117,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000062 DASRYE010000000
VERSION     DASRYE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233117"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VFF98_03335"
     CDS             complement(<1..1313)
                     /locus_tag="VFF98_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HZU62693.1"
                     /translation="MRKNPQAADYRPEPAIAPLAPWLADAVAPARFPAHRLRWRNDRW
                     APAVGLAALSEEAWIAHFGRFEPLEGNLPQPLALRYHGHQFRVYNPQIGDGRGFLFAQ
                     LRDSAGRLLDLGTKGSGTTPWSRRGDGRLTLKGAMREILATEMLEALGANTSKTFSVI
                     ETGEELWRGDEPSPTRSAVLVRLSHGHIRIGSFQRLLALEEAGHMAELVAYCLARFPG
                     PPPPADAPGADQPAVILLHQVVERLADMAASYVAAGFVHGVLNTDNMNISGESFDYGP
                     WRWLPRWEAGFTAAYFDEAGLYAFGRQAEAIRWNCGQLAIALNLLVGDTAPLIAALER
                     FLPLFHAHLARRVRWRLGVESAGGADEEADVALLSALGSAMQESDAMPCHAFHAHRGG
                     RGATGALAAALAPFRPVAGALDHPLWQEAAPPTNHIDEVERLWSA"
     gene            1399..2271
                     /locus_tag="VFF98_03340"
     CDS             1399..2271
                     /locus_tag="VFF98_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62694.1"
                     /translation="MTTGFIDAFWQGPDGLKLHYRDYPGDGGRPPLLCLPGLTRNARD
                     FEALAGRLAGRWRVICPDMRGRGDSDYARNSATYNPMQYVADVNALLEGLEIGRFVAV
                     GTSLGGLMTMILAMVEAGRIAGAVLNDIGPVIEPAGLARIRGYVGQGRNFPTWIHAAR
                     ALAEEQAHAYPDYTLDDWLAMAKRLMVVGGNGRIVFDYDMRIAEPFEKPGGEAGVDMW
                     PGLEALAGRPVLCLRGALSDILSAATLAEMSRRLPEAECLTLPQVGHAPTLAEPAAAA
                     AIDRLLERIAGAGA"
     gene            2268..3434
                     /locus_tag="VFF98_03345"
     CDS             2268..3434
                     /locus_tag="VFF98_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62695.1"
                     /translation="MSVGEEGRPLHILHLHSSFDPGGKELRSVQLINAFGTGASHTIV
                     SALPGAMAAAARIAPGIGWAQGLDFPPLQGRPTPARLRALASAMRGHDLVLTYNFGAM
                     DAVLAHTLFAQIDRLPPLVHHEDGFNQDEAEGLKPLRNWYRRIALGRARALVVPSARL
                     EAIALGPWRQPRGKVVRIANGIATGAFAGKPRPDALPRVIKRPGELWLGTIAGLRPVK
                     NLPAMVRAFAALPEPWQLVILGEGEAREAIRAEALRLDLGHRVHLPGFVAEPAKVAGL
                     FDLFALSSHSEQFPISLVEAMAAGLPVAATDVGDVAGMVAPENRPFVVPAGDEAALAA
                     AFAALAGDAALRARLGAANRARARAEYDEAAMIAAYRLVYGQALGRAGAAPAFP"
     gene            3557..4375
                     /locus_tag="VFF98_03350"
     CDS             3557..4375
                     /locus_tag="VFF98_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU62696.1"
                     /translation="MVLRSSRPQTRADQLAERQAAQNDAFLREVDDALREDNVLGALR
                     RHGKLALAVVVLGLGGLGGWLWWDNHQQDVAAQRSEKLVVALDQIQAGRADMAAPTLA
                     ELAASGVAGPAALARLEQAGIALQQNKADQAATLYAAVAGDAGAPQPYRDFANIRYVN
                     LKFDQLKSEEVVARLKPLAVPGKPWFGAAGELVAMAYLAQGHKELAAPLLGAIAKDTT
                     QPETLRARTRQIAGTLGFDAIDDIAKAPAVPAAAQPAPAPQAAPAPAATAPAKP"
     gene            4411..5778
                     /locus_tag="VFF98_03355"
     CDS             4411..5778
                     /locus_tag="VFF98_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZU62697.1"
                     /translation="MKPFTSSPRKGRSLSRWLALPLLGALAACSHGGPIHTKPNRPAT
                     PTVGNRIPILSNVESAARADTTIADVAVVLPTQTLNPDWAQPGGSASKSYGNLTLADK
                     PHHAWSATIAGTTQRQRLAAAPVVGGGLLYAMDTDGVVHAFDAVKGSHVWQHSFEIMG
                     GEASALFGGGASYDGGKLFVDTGLGDVAALDAKTGKLLWKVHPAGPLRGSPTVAYGMV
                     FVMTQNNEIHALAVEDGKEQWQETASLTATGVFGVAAPAAGQGTVVAGYASGELIAYR
                     YENGRTLWSDALARTSLSTSVGVLTDIDADPIIDRGRIYALGQGGRMAAYELVTGQRI
                     WELNIAGISTPAVAGDWIFTLTDDGKLLCIERNQGKVRWALQLDRFHNMKHKKQPLYW
                     TGPVLAGDKLWLADTAGDLYTVSTMDGVAKKYLSLKQPVSLAPVVAGGILYVLDDSGR
                     ITALR"
     gene            5851..7185
                     /locus_tag="VFF98_03360"
     CDS             5851..7185
                     /locus_tag="VFF98_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine methyltransferase"
                     /protein_id="HZU62698.1"
                     /translation="MAADRPLARCSSAPPAGDVSPWVGLVGLCGLGCWLALCRFWPQV
                     VDGLGLAAPRAVMNGPDAALLGLLCAALPMAAWQLLVDKVHRRPSTGLQWQAPRRWGE
                     GRGDSAVKLLGLWSAWALIGAAYWLGRWYGQGPYLFAMRVIGAAVVPLMLLSIPYVLW
                     LDRHLVERRCGAWHLGALLLGRAGACRAEALKFLRGWAVKGFFSAFMLSIVPGGFAEV
                     VSVDWGLVRAEPARLAGWLTAFLFMVDVQIGTVGYLFTLRPLDAHIRSANPHLDGWLA
                     ALICYPPFVLMNAGGPLDYHAGGADWGFWLAGRPALAWGWGAALVGLTAIYAAATLAF
                     GIRFSNLTWRGTITCGPYRWCRHPAYLSKNLFWWGASLPFLVRHGDAAEGWRNAALLG
                     LTGAVYWWRAKTEERHLLAEDPTYRAYWAWSEAHAPVTRALKQLRRVLFPRG"
     gene            complement(7182..7481)
                     /locus_tag="VFF98_03365"
     CDS             complement(7182..7481)
                     /locus_tag="VFF98_03365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZU62699.1"
                     /translation="MIVVLASFRIPADHVAEARALLPAVIATTLTEEGCQAYDVGEDV
                     LAPGLFRVSERWESRAALAAHAAAPHMAEWGRQRAAMGMSERRVTIFDIAGMEDA"
     gene            complement(7481..8869)
                     /locus_tag="VFF98_03370"
     CDS             complement(7481..8869)
                     /locus_tag="VFF98_03370"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZU62700.1"
                     /translation="MSTRQVSDAQEPFITAIDQLAAPMAAGEKPRARWRIGTEHEKFV
                     YRTADHRAPSYAEPGGIRDLLLGLKAFGWEEIFEDGANGQANVIAMKGADGTVSLEPA
                     GQLELSGAPLVNLHETCAESARHIAQVKAVSEATGTAFLGLGLWPDKTREELPVMPKG
                     RYEIMLRHMPRVGSMGLDMMLRTCTIQTNLDYASEADMVQKFRVSLALQPLATALFAN
                     SPFLEGKPNGFLSYRSHIWSDTDPARTGMLPFVFEEGFGYERYVDYMLDVPMYFVFRD
                     GRYIDAAGQSFRDFLAGKLPALPGELPRESDWIDHLSTAFPEVRLKSFLEMRGADGGP
                     TARICALPALWVGLLYDQAALDAAWDLVKGWDMEAREALRNAAPKLALDAPIPGGKTL
                     RDIAPEVLDIARAGLKARGQRNAIGDDETGYLAPLDDIVRSGKVPAQRLLDLYNGPWG
                     GDLSRIYEEKSF"
     gene            complement(8997..9749)
                     /locus_tag="VFF98_03375"
     CDS             complement(8997..9749)
                     /locus_tag="VFF98_03375"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZU62701.1"
                     /translation="MPATPAWPPRSAPRLFVAPPLAAGAEVAIEGPQAHYLARVMRLG
                     VGDAVILCDDASGEWAARAVSVGKRDVVLACVERLRPREEVPDFTLCAALLKKDRFDL
                     VLEKACELGAARIQPVLTRRCVADRLNMERARATLVEAAEQCARTALPALAEPVKLEA
                     LLKGWPAPRALFFADETGGAPAAPAFAAHAGPAALLTGPEGGFTSEERAAIRALPQAR
                     AITLGPRILRGETAAIGAMALWMGTMGDWQQG"
     gene            9824..10753
                     /gene="ubiA"
                     /locus_tag="VFF98_03380"
     CDS             9824..10753
                     /gene="ubiA"
                     /locus_tag="VFF98_03380"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZU62702.1"
                     /translation="MAGQIVPDSEHRGIMAILPAAARDYASLARLDRPIGWWLLYWPC
                     VWGVALAGGLAASGGARWGLLAWLLLGAVVMRGAGCVLNDIVDADIDIKVERTAQRPV
                     ASGRVSARQAWAWLVALCLVGLVVLLQLHWRAQLVALGSLALVAAYPFMKRITGWPQA
                     WLGLVFTWGAPVGWVEVRGFAGLDVLAALYGGAILWCIGYDTIYACQDREDDALIGIG
                     SSALSLGRHVRAGVLGFYAGALGLWGLALWHVRADPLALLALAPVALHLGWQAITLRP
                     EDGPGALARFRANRFAGLLMALACAVVGSVAAG"
     gene            complement(10750..11811)
                     /locus_tag="VFF98_03385"
     CDS             complement(10750..11811)
                     /locus_tag="VFF98_03385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU62703.1"
                     /translation="MPQAPAPPIAPPAGAPCQTPASQHPARSAQRYASLQMLRAVAAY
                     AVLLFHLGLFRLGYAGVDVFFVLSGFIMGMLGTQEGARAFLLRRLIRIAPLYWAVTLA
                     FCAITAVPGMARHFVLHGADLLRSLLFVPYVNAQGEIWPIVVPGWTLDYEILFYALMG
                     LCLAGCRRAAPQVLVAVLLALVAAGALLQPAAAPAQVLTGPLLLEFLFGLLLCASRPL
                     WGRWEGLVALVLGLAAFAFWAARGEADFTGFHRIATLGLASALLVGGAIGLEQGGAGT
                     AWRPLALLGRAAERMGDASYSLYLLHPLALPFVEKLLHGQPLLRPVVALALSSAVALA
                     AFRWFERPVGKALRRALRA"
     gene            complement(11864..15298)
                     /gene="smc"
                     /locus_tag="VFF98_03390"
     CDS             complement(11864..15298)
                     /gene="smc"
                     /locus_tag="VFF98_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZU62704.1"
                     /translation="MRFRRLKLSGFKSFVEPAELHIEPGLTGVVGPNGCGKSNLLEAI
                     RWVMGEQSSKSLRGAGMEDVIFAGTASRPARSFAEVVLLAETAEGEDLEVVRRIERGA
                     GSAYRLNGRDARQKDVALVFADAATGAHSPALVSQGRIAAVIAARPEERRAMLEEAAG
                     IAGLHVRRRDAEQRLRATEANLARLDDLMAGLDQQIAGLRRQARAAERYRDLSQRVRG
                     AEARVVYARWRAAADAAEAARAEAARTEGAARTAQEAAHAAQTAQAAAATLLAATRDS
                     HFEQRDEVSALGHQVATLSGQLEAARQRLADLDRQHARLEQDRADADRLTLDAAGALT
                     RLETELSEAKIRLAVEEARRPGLVSAQEKAEHTARAAELALAETNARQAALAADWRVA
                     NAEVAAVETRLARLEGEVARHGATRAGLEREGDGEAALAEARARREGASARIADLRTA
                     LATAQARKAEAQAARDAASTAQAAARADLAVVEREHQALAKDRDARARASSAAAGRRL
                     ALDHVRAEPGYERALAAVLGRDAKAPLGAAPPAAEGRFWTGAEAPPPVAGTLAAHVPD
                     APAELAARLALVHVAPADDGRALAPGEWLVTLAGHVRRWDGFVARGEGAAEAARLEAD
                     NRLAALEGQLPPLRAALAAAEMAAASAQAALAEAGPAAIAAERSLAGAAEAERTALRA
                     VDQAEALRERQAQRRADLARVEADLAAQRQQIETELASARAARAGLPGPDSIAAELAS
                     ARAAHETARTALQSAIAGLAGQDQLLAAARERSLSQAAEIRSWQARAGDAARRLAQMQ
                     GRTEEIASERAVVAAKPERLAEALARAQGARAAMAQRLAETEATLAAQEAAARAADAA
                     LANAQEQLASAREARAASAARAEAEDARRAEAARLSGERFQCPPPLLAERFGFAGADV
                     AGPEAEAAAHERLVAERDRLGPVNLVAAEELGEAETRAAQSLREREELAEAVNRLRGS
                     IGNLNREGRERLRAAFEAVDGHFRRLFTTLFQGGQAHLALIDSDDPLEAGLEIFAQPP
                     GKRLQSLTLASGGEQALTAVALIFALFLTNPSPICVLDEVDAPLDDANIERFCDLLEA
                     MVRETDTRYLIVTHNAVTMSRMHRLFGVTMVEKGVSRLVSVDLGGAEQLLAEPVSI"
     gene            complement(15305..16069)
                     /locus_tag="VFF98_03395"
     CDS             complement(15305..16069)
                     /locus_tag="VFF98_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU62705.1"
                     /translation="MMTAHRFVKAPLLAAGALTLALALSACKKDAAPAATGTVSGAPV
                     AAAAPPAGKAWSDVVAATADGGMVMGNPNAPIKLVEYGSLSCPHCAKLANEGMSKLVG
                     TYVASGKVSYEYRSFAIHSVDIPLTVLVRCASPDAFFGLVEQLYTNQDALLTRAQANE
                     AAAKQAASLPPAQRLVAMSDALGFTEFFSARGLATDQAHKCLADTTAAEKVAKEAEAT
                     GAAGIDSTPTLLINGTKQTALTWPELEAALQTAGAR"
     gene            complement(16119..16847)
                     /locus_tag="VFF98_03400"
     CDS             complement(16119..16847)
                     /locus_tag="VFF98_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZU62706.1"
                     /translation="MPLLRVLLALAMAAGLAPAALAQPAPAHHAAPARPVPPPPGGWL
                     AQVSATADGAMVLGNPHAQWRITEYMSYTCPHCAHFEVEGGPPLRLGAIAPGKAALEV
                     RHFLRDPLDLTVALLTHCASGPAFFALHQSFLARQDEWMARAQSATPAQRQRWENGPL
                     AQRTRAIASDLHFYDIMESRGIERPRADRCLANEALAKQLSAQTARAEQAGVSGTPSF
                     ALNGELLAGTHDWATLQLQLNAHM"
     gene            complement(16847..17512)
                     /locus_tag="VFF98_03405"
     CDS             complement(16847..17512)
                     /locus_tag="VFF98_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZU62707.1"
                     /translation="MEPEPPASSPPAPPEGPKPAGRKAKAAGTKGAGKASAGKAKKAP
                     QRPYERPRGGPARAIADLMPDIGRSAFRRFGFVQSSIVTRWPEIVGAAQARVCAPEAI
                     RFPAGERCEGTLQLVVRPGHAPLIAHAESEIIARVNRFFGYNAVARIRLRQGEVAEPP
                     SPARPAQPPALRPVPVELGDSLRDIGDPELRAVLESLARSLAAGNASAGREQAPPAKG
                     KKA"
     gene            17542..18624
                     /locus_tag="VFF98_03410"
     CDS             17542..18624
                     /locus_tag="VFF98_03410"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZU62708.1"
                     /translation="MDSRADLALAPRTDPAALLLAWYDAHARSLPWRAPPGAPPMDAY
                     RVWLSEVMLQQTTVAAVIPYFQRFTQAWPDVLALAAAPEEAVMAAWAGLGYYSRARKL
                     VECARVVAANGGAFPQEEEALLALPGLGPYTAAAVAAIAFGRRAVVVDANVERVVARY
                     GAIGAPLPAAKPAIRAATDALTPAERPGDFAQAMMDLGASLCSVRAPKCLLCPLAGGC
                     AARAAGTAEAYPVKPPKKPKPQRRGRAFWITREGAAGAEVWLVRRPGRGLLGGMRALP
                     DNGWNARADGAGPPPLAGPWQAGPSVAHVFTHFALSLEVAFYSGKEAASLATGAGEWW
                     PLARLDEAGLPSLFARAAMAARAMRA"
     gene            18708..19616
                     /gene="nudC"
                     /locus_tag="VFF98_03415"
     CDS             18708..19616
                     /gene="nudC"
                     /locus_tag="VFF98_03415"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZU62709.1"
                     /translation="MRDTHAPRAVAFAGGVLDRADPVRADPAALAALAAHGRLLLLDG
                     LEPLLGEGGALCSAPVGPDAGELIFLGRDEAGIGWFIAAPDAASASTAPPNPALWEAI
                     ASMAPADLALYGTARSLAGWHARHRFCARCGAPTRLAKGGWQRSCASEACAADHFPRT
                     DPVVIMSVAHEGRLLLGRQPRFPPHRYSALAGFIEPGEAVEEAVAREIWEEAGLRVAD
                     VRYVASQPWPFPSSLMLACHAEALDDAITIDTTELEDARWFTRAEVAEAMAARARGAD
                     GQAFSAPPRHAVAWHLLAHWLEQGAG"
     gene            19727..>20189
                     /locus_tag="VFF98_03420"
     CDS             19727..>20189
                     /locus_tag="VFF98_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62710.1"
                     /translation="MTASGLKSRPSVRTAARPRPAARALAAAPALAVPAALPRPPQAP
                     ARGTLEALLGLLAWWPLPLLLAAIVAVLAWQGWEMMRFRSMAVPGGIAVQTEGTRATI
                     TGGRVYVELPVLVSNSTDSPIREVALWARVYACPSAGAQVADCRLLLSAEQD"
BASE COUNT         2736 a         6852 c         7529 g         3072 t
ORIGIN      
        1 atggcggacc acaggcgctc cacctcgtcg atatggttgg tgggcggggc ggcttcctgc
       61 cacagcggat gatccagcgc gccggccacc gggcggaaag gcgccagcgc cgcggccagc
      121 gcgccagtgg cgccgcgccc gccgcgatgg gcgtggaagg catggcaggg catggcgtcg
      181 ctttcctgca tggcgctgcc aagggcggac agcagcgcca catcggcctc ctcatcggca
      241 ccgcccgcgc tttccacccc cagccgccag cgcacgcgcc gggccagatg ggcgtggaac
      301 agcggcagga agcgttccag cgcggcgatc agcggggcgg tgtcgcccac cagcaggttg
      361 agggcgatgg ccagctgccc gcagttccag cggatggcct cggcctgccg gccaaaggca
      421 tagaggccgg cctcgtcgaa ataggcggcg gtgaagccgg cttcccagcg cggcagccag
      481 cgccaggggc cataatcgaa gctctcgccc gagatgttca tgttgtcggt gttgagcacg
      541 ccgtgaacga agccggcggc cacatagctg gcggccatgt cggccaggcg ttccaccacc
      601 tgatggagca ggatgaccgc cggctggtcg gcgccggggg catcggcggg cggcgggggg
      661 ccgggaaagc gggccaggca ataggccacc agctcggcca tgtggccggc ctcttccagc
      721 gccagcagcc gctggaaact gccgatgcgg atgtgcccgt gcgacaggcg caccagcacc
      781 gccgaacggg tggggctggg ctcgtcgccg cgccacagtt cctcgccggt ctcgatcacc
      841 gagaaggtct tgctggtgtt ggcgcccagc gcttccagca tttcggtggc caggatctcg
      901 cgcatggcgc ccttcagcgt cagccgccca tcgccccggc gcgaccacgg cgtggtgccc
      961 gaacccttgg tgcccagatc cagcaggcgc ccggccgaat cgcgcagctg ggcaaagagg
     1021 aagccgcgcc cgtcgccgat ctgggggttg tagacgcgga actgatggcc atgataacgc
     1081 agcgccagcg gctggggcag attgccttcc agcggctcga aacggccgaa atgggcgatc
     1141 cacgcttcct cggaaagggc cgcgaggccc accgccggcg cccagcgatc gttgcgccag
     1201 cgcagccggt gggcggggaa gcgggcgggg gccaccgcat cggccagcca gggggcaagc
     1261 ggggcgatgg cgggctctgg ccgataatca gcggcttgcg ggtttttgcg catgcggcaa
     1321 taagtggccc tgccgggggg ctggcgcaag gggctatggc ggatggcgcg ctgtcatcgc
     1381 ctgtggaaag tgacgggcat gacgacgggt ttcatcgatg cgttctggca ggggcctgat
     1441 gggctgaagc tgcattatcg cgattatccg ggcgatgggg gccggccgcc gctgctgtgc
     1501 ctgccgggcc tcacgcgcaa tgcccgcgat ttcgaggcgc tggccgggcg gctggcgggc
     1561 cgctggcgcg tgatctgccc cgatatgcgc gggcgcggcg acagcgatta tgcccgcaac
     1621 agcgccacct acaatccgat gcaatatgtg gccgatgtga acgccctgct ggaagggctg
     1681 gagattggcc gctttgtggc ggtgggcaca tcgctgggcg ggctgatgac gatgatcctc
     1741 gccatggtgg aggctggccg cattgccggc gccgtgctga acgacattgg cccggtgatc
     1801 gagccggcgg gcctggcgcg cattcgcggc tatgtggggc aggggcgcaa ttttcccacc
     1861 tggatccatg ccgcccgcgc gctggccgag gagcaggccc atgcctatcc cgactacacg
     1921 ctggacgact ggctggcgat ggccaagcgg ctgatggtgg tgggcggcaa tggccgcatc
     1981 gtgttcgact atgacatgcg catcgccgag cctttcgaga agccgggcgg cgaggcgggg
     2041 gtggatatgt ggcccgggct ggaggcgctg gccggccggc cggtgctgtg cctgaggggc
     2101 gccctgtccg atatcctctc ggccgcaacg ctggccgaga tgagccgccg cctgccagag
     2161 gcggaatgcc tgaccctgcc gcaggtgggc catgccccca cgctggcaga gccggcagcg
     2221 gcagccgcga tcgaccggct gctggaacgt atcgccgggg ccggggcatg agcgtgggcg
     2281 aagagggccg gccgctgcat atcctccacc tgcattccag cttcgatccg ggcggcaagg
     2341 aattgcgctc ggtgcagctg atcaacgcct ttggcacggg cgccagccac accatcgtct
     2401 cggccctgcc gggggcgatg gcggcggccg cgcgcattgc ccccggcatc ggctgggcgc
     2461 aagggctgga ttttccgccg ctgcagggcc ggcccacgcc cgcccggctg cgcgcgctgg
     2521 ccagcgccat gcgcgggcat gatctggtgc tcacctataa tttcggggcg atggatgcgg
     2581 tgctggcgca cacgctgttc gcccagatcg accggttgcc gccgctggtg caccacgagg
     2641 atggcttcaa ccaggatgag gccgaggggc tgaagccctt gcgcaactgg tatcgccgca
     2701 ttgcccttgg gcgggcgcgg gcgctggtgg tgccctcggc ccggctggag gcgattgcgc
     2761 tgggcccctg gcgccagccg cgcggcaagg tggtgcgcat tgccaatggc attgccaccg
     2821 gcgcctttgc cggcaagccc cggcccgatg ccctgccgcg ggtgatcaag cggccgggcg
     2881 aattgtggct gggcacgatc gccggcttgc ggccggtgaa gaacctgccg gccatggtgc
     2941 gcgcctttgc tgccctgcca gagccatggc agctggtgat cctgggcgag ggcgaggcgc
     3001 gtgaggcgat ccgcgccgag gcgctgcggc tggatctggg gcaccgggtg catctgcccg
     3061 gctttgtggc cgagcctgcc aaggtggccg gcctgttcga cctgttcgcg ctgtcgtcgc
     3121 acagcgagca gtttcccatc tcgctggtgg aggcgatggc ggccggcctg ccggtggcgg
     3181 ccaccgatgt gggcgatgtg gccgggatgg tggcgcccga gaatcgcccc ttcgtggtgc
     3241 cggcgggcga tgaggcggcg ctggcggccg cttttgcggc gctggcgggc gatgccgcct
     3301 tgcgtgcccg gctgggggca gccaatcgcg ccagggcccg ggcggaatat gacgaggcgg
     3361 cgatgatcgc tgcctatcgc cttgtttatg gtcaggcttt gggccgcgct ggggcggcgc
     3421 cggcctttcc ctgagggggc ggcccaccat aggcgttgaa atgcccggcc tatccgctaa
     3481 acaccggcca gttttgcctt gtctggcagg aggcctgcgg gcacccttgt ccagttgtta
     3541 agaaagaagc cctgtcttgg tcctgcgttc cagccgtccg caaacccgtg ccgaccagct
     3601 tgccgagagg caggccgcgc aaaacgacgc tttcctgcgc gaggtggacg atgccctgcg
     3661 cgaggataac gtgctgggcg cgctgcgccg ccatggcaag ctggcgctgg cggtggtggt
     3721 gctgggcctt ggcgggctgg gcggctggct gtggtgggat aaccaccagc aggatgtggc
     3781 cgcgcagcgc tcggaaaagc tggtggtggc gctggaccag atccaggccg gccgcgccga
     3841 catggccgcg cccacgctgg ccgaactggc cgcctcgggc gtggcgggcc cggcggcgct
     3901 ggcccggctg gaacaggccg gcattgccct gcagcagaac aaggccgatc aggccgcaac
     3961 gctctatgcc gcggtggccg gcgatgccgg cgcgccccag ccctatcgcg attttgccaa
     4021 tatccgctat gtgaacctga agttcgacca gctgaagagc gaggaggtgg tggcccgcct
     4081 caagccgctg gcggtgccgg gcaagccgtg gtttggcgcg gcgggcgaac tggtggcgat
     4141 ggcctatctg gcgcagggcc acaaggaact ggccgccccc ctgctgggcg ccatcgccaa
     4201 ggataccacc cagcccgaaa cgctgcgcgc ccgcacccgc cagatcgccg gcacgctggg
     4261 cttcgatgcg atcgacgata tcgccaaggc cccggctgtg cccgccgctg cccagccggc
     4321 cccggccccg caggcggccc ctgcgcccgc cgccaccgcc cccgccaagc cctgaacaca
     4381 cacgcttttc cacggatagg acctgagcgc atgaagccct tcacctcttc gccccgcaag
     4441 ggccgcagcc tctcccgctg gctggcgctg ccgctgcttg gcgcgctggc ggcctgttcg
     4501 catggcgggc ccatccacac caagcccaac cgcccggcca cgcccaccgt gggcaaccgc
     4561 atccccatcc tcagcaatgt ggaaagcgcc gcgcgcgccg ataccaccat cgccgatgtg
     4621 gccgtggtgc tgcccaccca gacgctgaac cccgattggg cccagcccgg cggcagcgcc
     4681 agcaagagct atggcaacct gaccctggcc gacaagcccc accacgcgtg gagcgccacc
     4741 atcgccggca ccacccagcg ccagcgcctg gctgccgcgc ccgtggtggg cggcggcctg
     4801 ctctatgcga tggacaccga tggcgtggtg catgccttcg atgccgtgaa gggcagccat
     4861 gtgtggcagc acagcttcga gatcatgggc ggcgaggcat cggcgctgtt tggcggcggc
     4921 gccagctatg atggcggcaa gctgttcgtc gatacagggc tgggcgatgt cgccgcgctg
     4981 gatgccaaga ccggcaagct gctgtggaag gtgcacccgg ccggcccgct gcgcggctcg
     5041 ccgacggtgg cctatggcat ggtcttcgtg atgacgcaga acaacgagat ccatgcgctg
     5101 gccgtggaag atggcaagga gcagtggcag gaaaccgcct cgctcaccgc caccggcgtg
     5161 ttcggcgtgg cagcgccggc ggccggccag ggcacggtgg tggccggcta cgcctcgggc
     5221 gagctgatcg cctatcgcta cgagaacggc cgcacgctgt ggtcggacgc gctggcgcgc
     5281 acctcgcttt ccaccagcgt tggcgtgctg accgatatcg acgccgaccc gatcatcgat
     5341 cgcggccgca tctatgcgct gggccagggc gggcgcatgg ccgcctatga gctggtgacc
     5401 ggccagcgca tctgggagct gaacatcgcc ggcatttcca ccccggcggt ggccggagac
     5461 tggatcttca ccctgaccga cgatggcaag ctgctgtgca tcgagcgcaa ccagggcaag
     5521 gtgcgctggg cgctgcagct cgatcgcttc cacaacatga agcacaagaa gcagccgctc
     5581 tactggaccg gcccggtgct ggccggcgac aagctgtggc tggccgatac ggcgggcgat
     5641 ctctacaccg tctcgacgat ggatggcgtg gcgaagaaat atctctcgct gaagcagccc
     5701 gtgtcgctgg caccggtagt ggccggcggc atcctctatg tgctggacga tagcgggcgg
     5761 atcaccgcgc tgcgttaacc ggccgcaggc ccagggggcg gcgcggcgtg gtttgcgcgc
     5821 ccttaaccct ttggcattag aggggcgatc atggctgccg atcgccccct tgcccgttgt
     5881 tcaagcgcgc cgccggcggg cgatgtgtcg ccctgggtgg ggctggtggg cctgtgcggg
     5941 ctgggctgct ggctggccct gtgccgcttc tggccgcagg tggtggatgg gctgggcctt
     6001 gcagcccccc gcgcggtgat gaacgggccg gatgcggcgc tgctgggcct gctgtgcgcc
     6061 gccctgccga tggccgcctg gcagctgctg gtagacaagg tgcaccgccg gccttccact
     6121 ggcctgcagt ggcaggcgcc gcgccgctgg ggcgaggggc ggggcgacag cgcggtcaag
     6181 ctgctgggcc tgtggagcgc ctgggcgctg attggcgcgg cctattggct ggggcgctgg
     6241 tatggccagg gcccctatct gtttgccatg cgggtgatcg gcgcggcggt ggtgccgctg
     6301 atgctgctgt ccatccccta tgtgctgtgg ctggatcgcc atctggtgga gcggcgctgc
     6361 ggcgcctggc atctgggcgc gctgctgctg ggccgggccg gggcctgccg ggcagaagcg
     6421 ctgaagttcc tgcgcggctg ggcggtgaag gggtttttca gcgccttcat gctctccatc
     6481 gtgccgggcg gctttgccga ggtggtgtcg gtggattggg gactggtacg ggcagagccg
     6541 gcgcggctgg ccggctggct cacggctttc ctgttcatgg tggatgtgca gatcggcacg
     6601 gtgggctatc tgttcaccct gcgcccgctg gatgcgcata tccgcagcgc caatccgcat
     6661 ctcgatggct ggctggccgc gctcatctgc tatccgccgt ttgtgctgat gaatgccggc
     6721 gggccgctgg attatcacgc agggggcgcc gattggggct tctggctggc cgggcggccg
     6781 gcgctggcct ggggctgggg ggcagcgctg gtggggctga cggcgatcta tgcggcggca
     6841 acgctggcct ttggtatccg cttctccaat ctcacctggc gcggcacgat cacttgcggg
     6901 ccctatcgct ggtgccgcca cccggcctat ctgtcgaaga acctgttctg gtggggggcc
     6961 agcctgccct ttctggtgcg ccatggtgat gcggccgagg gctggcgcaa tgccgccctg
     7021 ctggggctga cgggcgctgt ctactggtgg cgggcgaaaa ccgaggagcg ccacctgctg
     7081 gccgaagacc ccacctatcg cgcctattgg gcgtggagcg aggcccacgc cccggttacg
     7141 cgggcgctga agcagctgcg gcgagtgctt ttcccgcggg gttaggcgtc ttccatcccg
     7201 gcgatgtcga agatcgtcac ccggcgctcg ctcatcccca tggcggcgcg ctggcggccc
     7261 cattcggcca tgtgcggggc ggcggcatgg gcggcaagcg cggcgcggct ctcccagcgt
     7321 tccgacacgc ggaagaggcc aggcgccagc acatcctcgc ccacgtcata ggcctggcag
     7381 ccttcttcgg tcagcgtggt ggcgatcacg gcgggcagca gcgcgcgggc ctcggccaca
     7441 tggtctgccg gaatgcggaa gctggcgaga acgacgatca tcagaagctc ttttcctcgt
     7501 agatgcgcga aagatcgccg ccccaggggc cgttgtagag atcgagcagg cgctgcgccg
     7561 gcaccttgcc gctgcgcacg atatcgtcga gcggggcgag atagccggtt tcatcatcgc
     7621 caatggcgtt gcgctggccg cgtgccttca gccccgcgcg ggcgatgtcg agcacttcgg
     7681 gggcgatgtc gcgcagggtc ttgccgccgg ggatgggggc atcgagcgcc agcttgggcg
     7741 cggcattgcg cagggcctcg cgcgcttcca tgtcccagcc cttcaccaga tcccaggccg
     7801 catccagcgc cgcctgatca tagagcaggc ccacccacag cgccggcagc gcgcagatgc
     7861 gcgcggtggg gccgccatcg gcgccgcgca tttcaaggaa gcttttcagc cgcacttcgg
     7921 gaaaggccgt ggagagatgg tcgatccagt cgctctcgcg cggcagttcg ccgggcaggg
     7981 cgggcagctt gccggcgaga aaatcgcgga aggactggcc cgccgcatcg atataccggc
     8041 catcgcggaa gacgaaatac atcggcacgt cgagcatata atcgacatag cgttcgtagc
     8101 cgaagccctc ctcgaagacg aagggcagca tgccggtgcg tgccggatcc gtgtcggacc
     8161 agatgtggct gcgataggaa aggaagccgt tgggcttgcc ttcaaggaag ggcgaattgg
     8221 cgaacagcgc ggtggccagc ggctgcagcg ccaggctcac gcggaacttc tgcaccatat
     8281 ccgcctcgct cgcataatcg aggttggtct ggatggtgca ggtgcgcagc atcatgtcga
     8341 ggcccatgct gcccacgcgc ggcatgtggc gcagcatgat ctcgtagcgg cccttgggca
     8401 tcaccggcag ctcttcgcgg gtcttgtcgg gccacaggcc aaggccgagg aaggcggtgc
     8461 cggtggcctc gctcaccgct ttcacctggg cgatgtggcg ggcgctttcg gcgcaggttt
     8521 catgcaggtt taccagcggc gcgcccgaca gttcgagctg gcccgccggc tccaggctga
     8581 ccgtgccatc agcgcccttc atggcgatga cattggcctg gccattggcg ccgtcttcaa
     8641 agatctcttc ccagccgaac gccttcagcc ccagcagcag atcgcggatg ccgcccggct
     8701 cggcatagga gggggcgcgg tgatcggcgg tgcgatagac gaatttctcg tgctcggtgc
     8761 cgatgcgcca gcgcgcgcgc ggcttttcgc cggcggccat gggggcggcc agctgatcga
     8821 tcgccgtgat gaacggctct tgcgcgtcgg atacctgcct ggtgctcatg gccgctgcga
     8881 tagagggcga aatggccccg cgccagcgca aaatgaacgg gggctgacag gcggatttca
     8941 agagggcggc gcaaattttg ggcggctgca catttgcgct tatggccccc ttgcgctcag
     9001 ccctgctgcc aatcgcccat ggtgcccatc cacagggcca tggcgccgat cgcggccgtc
     9061 tcgccgcgca ggatgcgggg cccaagggtg atggcgcgcg cctgtggcag ggcgcggatg
     9121 gcggcgcgct cctcgctggt gaagccgcct tcggggccgg tgagcaaggc cgccgggccg
     9181 gcatgggcgg caaaggcagg ggcggccggc gcgccgccgg tttcatcggc aaagaacagg
     9241 gcgcgcggcg ccggccagcc tttcagcagg gcctccagct tcaccggttc ggcaagggcg
     9301 ggcagggcgg tgcgggcgca ttgctcggcc gcttccacca gcgtggcgcg ggcgcgctcc
     9361 atgttcagcc ggtcggccac gcagcggcgg gtgagcaccg gctggatgcg cgcggcgccc
     9421 agctcgcagg ccttttccag caccaggtcg aagcggtcct tcttgaggag ggcggcgcac
     9481 agggtgaaat cgggcacctc ttcgcgcggg cgcaggcgtt ccacgcaggc cagcaccaca
     9541 tcgcgcttgc caaccgagac cgcccgcgcc gcccattcgc cgctggcatc atcgcacagg
     9601 atcaccgcat cgcccacgcc caggcgcatc acccgggcga gataatgcgc ctgcgggcct
     9661 tcgatggcca cttcggcgcc cgctgccagc ggcggggcaa cgaacaggcg cggggcagag
     9721 cgcggcggcc aggcgggggt ggcgggcagg tggtcggtca tgcgggaagg ccctagccga
     9781 tgcgtgcgcg cgccgctatg ccccagcccc gaggagactg cagatggccg gccagatcgt
     9841 acctgatagc gagcaccgcg ggatcatggc gatcctgccc gcagccgcgc gcgattatgc
     9901 ctcgctggcc cggctggacc ggccgatcgg ctggtggctg ctctattggc cctgcgtgtg
     9961 gggagtggcg ctggcgggcg ggctggcggc atcgggcggc gcgcgctggg ggctgctggc
    10021 ctggctgctg ctgggcgcgg tggtgatgcg cggggccggc tgcgtgctga acgacattgt
    10081 cgacgccgat atcgacatca aggtggagcg caccgcccag cggccggtgg ccagcgggcg
    10141 ggtgtcggcc cggcaggcct gggcgtggct ggtggcgctg tgcctggtgg ggctggtggt
    10201 gcttttgcaa ttgcactggc gggcgcagct ggtggcgctg ggctctctgg cgctggtggc
    10261 ggcctatccc ttcatgaagc gcatcaccgg ctggccgcag gcgtggctgg ggctggtgtt
    10321 cacctggggc gcgccggtgg gctgggtgga agtgcgcggc tttgccgggc tggatgtgct
    10381 ggccgcgctg tatggcgggg cgatcctgtg gtgcatcggc tatgacacga tctatgcctg
    10441 ccaggaccgc gaggatgatg cgctgatcgg catcggttcc tcggcgctgt cgctggggcg
    10501 gcatgtgcgg gccggggtgc tgggctttta tgcaggggcg ctgggcctgt gggggctggc
    10561 gctgtggcat gtgcgggccg atccgctggc gctgctggcg ctggcgcctg tggcgctgca
    10621 tctgggctgg caggcgatca ccctgcggcc ggaggatggg ccgggggcgc tggcgcggtt
    10681 tcgcgccaat cgctttgctg gcctgttgat ggcgctggcc tgcgcggtgg tgggcagcgt
    10741 ggcagctggc taggcgcgca gggcccggcg cagggccttg cccaccggcc gttcaaacca
    10801 gcggaacgcg gccagcgcca ccgcgctgct gagcgccagc gccaccaccg ggcgcagcag
    10861 cggctgccca tgcagcagct tttccacaaa gggcagcgcc agcgggtgca gcagatagag
    10921 cgaataggac gcatcgccca tccgctctgc cgcgcggccc agcagcgcca gcgggcgcca
    10981 ggcggtgcca gccccgccct gttccagccc gatcgccccg cccaccagca gggcagaggc
    11041 cagccccagc gtggcgatgc ggtgaaatcc ggtgaaatcc gcctctcccc gcgccgccca
    11101 gaaggcaaag gccgccagcc ccagcaccag cgccaccagc ccttcccacc gcccccacag
    11161 cggccggctg gcgcacagca gcaggccgaa caggaattcc agcagcagcg gcccggtcaa
    11221 cacctgcgct ggcgctgccg ccggctgcag cagcgcgccc gccgccacca gcgccagcag
    11281 caccgccacc agcacctgcg gcgcggcgcg gcggcagcct gccaggcaca ggcccatcag
    11341 cgcgtagaac aggatctcgt aatccagcgt ccaccccggc acgacgatcg gccagatctc
    11401 cccttgcgcg ttgacatagg ggacaaacag caaggagcgc aggagatcgg ctccatgcag
    11461 cacgaaatgg cgcgccatgc ccggcaccgc ggtgatggcg cagaaagcca gcgtaaccgc
    11521 ccagtaaagc ggcgcgatgc ggatcagccg gcgcagcagg aaagcgcgcg cgccctcctg
    11581 cgtgcccagc atgcccatga tgaagcccga cagcacgaag aacacatcca ccccggcata
    11641 gcccagccgg aacagcccca gatgaaacag cagcaccgca taggccgcca ccgcccgcag
    11701 catctgcaac gaggcatagc gttgagcgct gcgcgccggg tgctggctgg cgggcgtttg
    11761 gcatggggcg ccggctggcg gggcaatagg cggggcaggg gcttgcggca tggcgccagc
    11821 ctagtcgggc cgcgccggca atgccgcccc aactgcctcc ggatcagatg gataccggct
    11881 ctgccagcag ctgctcggcc ccgcccagat ccacgctgac aagccgcgag acgccctttt
    11941 ccaccatcgt cacgccgaac aggcggtgca tccggctcat cgtcacggca ttgtgggtga
    12001 cgatgaggta gcgcgtgtcg gtctcgcgca ccatcgcttc cagcaggtcg cagaaacgct
    12061 cgatattggc atcgtcgagc ggcgcgtcca cctcgtcgag cacgcagatc ggcgaggggt
    12121 tggtgagaaa cagcgcgaag atcagcgcca ccgccgtcaa cgcctgctcg ccgcccgagg
    12181 cgagggtgag cgattgcagc cgcttgccgg gcggctgggc gaagatctcg aggcccgcct
    12241 ccagcggatc gtccgaatcg atcagcgcca gatgcgcctg cccgccctgg aacagcgtgg
    12301 tgaacagccg gcggaaatgg ccatccaccg cctcgaaggc cgcgcgcagc cgctcgcgcc
    12361 cctcgcggtt gaggttgccg atggagccgc gcagccggtt caccgcctcg gccagctcct
    12421 cgcgctcacg cagggattgg gcggcgcgtg tctccgcctc gcccagctcc tcggcggcga
    12481 cgaggttgac agggcccagc cggtcacgct cggccaccag ccgctcgtgg gcggccgctt
    12541 ccgcctcggg gccggcgaca tcggcgccgg caaagccaaa gcgttcggcc agcagcggcg
    12601 gcgggcactg gaaacgctcg cccgaaaggc gggcggcctc ggcgcggcgg gcgtcttccg
    12661 cctcggcgcg ggcggcgctg gcggcgcggg cctcacgcgc gctggccagc tgttcctggg
    12721 cattggcaag ggcggcatcg gcagcgcggg cggcggcttc ctgcgcggca agggtggcct
    12781 cggtttcggc caggcgctgg gccatggcgg cgcgggcgcc ctgggcgcgg gccagcgctt
    12841 cggccagccg ctccggcttg gcggccacca ccgcgcgttc gctggcgatc tcctcggtgc
    12901 ggccctgcat ctgggcgagg cggcgggcgg catcgccggc gcgggcctgc cagctgcgga
    12961 tctcggcggc ttgcgacagg ctgcgttcac gcgcggcggc cagcagctga tcctgcccgg
    13021 caaggccggc aatggccgat tgcagcgccg tgcgcgcggt ttcatgggcg gcgcgcgcgc
    13081 tggccagttc ggctgcgatg ctgtctggcc cgggcaggcc ggcgcgggcg gcgcgggcgc
    13141 tggccagctc ggtctcgatc tgctggcgct gggcggccag atcggcttcc acccgggcca
    13201 gatcggcgcg gcgctgggcc tgcctttcgc gcagcgcctc ggcctgatcc accgcgcgca
    13261 gggcggtgcg ctcggcctcg gcagcgccgg ccaggctgcg ctcggcagcg atggcggccg
    13321 ggcccgcctc ggccagagcc gcctgcgcgc tggccgctgc catctcggcc gcggcaaggg
    13381 cggcgcgcaa gggcggcaat tgcccctcca gcgcggccag gcggttgtcc gcctccagcc
    13441 gggcggcctc ggccgcgcct tcgccgcgcg ccacaaagcc atcccagcgc cgcacatggc
    13501 cggccagcgt gaccagccat tcgcccggcg ccagcgcgcg gccatcgtct gccggcgcca
    13561 catgcaccag cgcgaggcgg gcggcgagct ctgccggggc atcgggcaca tgggcggcca
    13621 gcgtgccggc cactggcggc ggggcctcgg cgccagtcca gaagcggccc tcggcggcag
    13681 ggggcgcggc gcccagcggc gccttggcat cgcgccccag cacagcggcc agcgcccgct
    13741 catagcccgg ctctgcccgc acatgatcga gcgcaaggcg ccggccggcc gccgcagagc
    13801 tggcgcgggc gcgcgcgtcg cggtctttcg ccagcgcctg atgctcgcgc tccaccacgg
    13861 caagatcggc ccgggcggcg gcctgcgcgg tgctggcggc atcgcgggcg gcttgcgcct
    13921 cggccttgcg ggcctgggcg gtggccagcg cggtgcgcag atcggcgatg cgcgcgctgg
    13981 cgccctcgcg ccgggcgcgg gcttcggcaa gggcggcctc gccatcgcct tcccgctcca
    14041 gcccggcgcg ggtggcgcca tggcgggcca cttcgccttc cagccgggcg aggcgcgttt
    14101 ccaccgccgc cacttcggca ttggccacgc gccagtcggc tgccagcgcg gcttggcggg
    14161 cattggtttc ggccagcgcc agctcggcgg cgcgggcggt gtgctcggcc ttttcctgcg
    14221 cgctgacgag gccggggcgg cgcgcttcct ccaccgcgag gcggattttg gcttccgaca
    14281 gttcggtttc cagccgggtg agggcgccgg cggcatccag cgtcagccgg tcggcatcgg
    14341 cgcggtcctg ctccagccgg gcgtgctggc ggtcgagatc ggccagccgc tggcgggcgg
    14401 cctccagctg gccggagagg gtggcgacct gatggcccag cgcgcttact tcatcgcgct
    14461 gttcgaaatg gctgtcacgc gtggcggcca gcagggtggc ggcggcggcc tgcgcggttt
    14521 gcgcggcatg ggcggcttcc tgcgcggtgc gggcggcgcc ctcggtgcgg gcggcttcgg
    14581 cgcgggcggc ctcggcggca tcggcagcgg cgcgccagcg ggcatagacc acccgcgcct
    14641 cggcgccgcg cacccgctgc gacagatcac gatagcgttc ggccgcgcgg gcctggcgcc
    14701 gcagcccggc aatctgctgg tcgagccctg ccatcaggtc gtccagccgg gccaggttgg
    14761 cctcggtggc gcgcaggcgc tgctcggcat cgcgccggcg cacatggagg ccggcaatgc
    14821 ccgccgcttc ctccagcatg gcgcgccgct cttccggccg cgcggcgatc accgcggcga
    14881 tgcgcccctg cgagaccagc gccggcgaat gggcaccggt ggccgcatcg gcaaacacca
    14941 gcgccacatc cttctgccgg gcatcgcgcc cgttcagccg ataggcgctg cccgcccccc
    15001 gctcgatccg gcggacaact tcaagatcct cgccctctgc ggtttcggcc agcagcacca
    15061 cttcggcaaa gctgcgcgcc gggcggctgg cggtgccggc aaagatcaca tcttccatgc
    15121 cggcgccgcg caggctcttg gagctttgct cgcccatcac ccagcggatg gcttccagca
    15181 gattggactt gccgcagcca ttggggccga caacgccggt cagcccgggt tcgatatgca
    15241 gttccgccgg ctcgacgaag ctcttgaaac ccgagagctt gagccggcgg aaacgcatgg
    15301 gggttcagcg cgcgccggcg gtctgcaggg ccgcttccag ctcgggccag gtcagcgcgg
    15361 tctgcttggt gccgttgatg aggagggtgg gggtggaatc gatgccggcc gcgccggtcg
    15421 cctcggcttc cttggccacc ttttcggcag cggtggtatc ggccaggcac ttgtgcgcct
    15481 gatcggtggc aaggccgcgc gccgagaaga attcggtgaa gcccagcgca tccgacatgg
    15541 ccaccaggcg ctgcgccggc ggcaggctgg cggcctgctt ggcggcggcc tcgttggcct
    15601 gggcgcgggt gagcagggca tcctggttgg tgtagagctg ctccaccagg ccgaagaagg
    15661 catcggggct ggcgcagcgc accagcacgg tcagcgggat atccacgctg tgaatggcaa
    15721 agctgcgata ttcatacgac accttgcccg aggcgacata ggtgccaacc agcttgctca
    15781 tcccctcatt ggccagcttt gcgcaatgcg ggcaagacag cgagccatat tcgaccagct
    15841 tgatcggtgc gttggggttg cccatcacca tgccgccatc ggcggtggcg gccaccacat
    15901 cgctccacgc cttgccggca gggggggcgg ccgcggccac cggcgccccg ctgacggtgc
    15961 ccgtggcagc gggggcagca tccttcttgc aggcgctgag ggccagcgcc agcgtcaggg
    16021 cgccggcggc cagcaggggg gctttcacga aacggtgtgc agtcatcatc tcttgtcctt
    16081 cgcgcagggc aggcttgttc ggggcggcgg gccaggcgtt acatgtgggc gttcagctgc
    16141 agttgcagcg tcgcccagtc atgcgtgccg gccagcagct cgccattgag ggcaaagctg
    16201 ggggtgccgg aaacgccggc ctgttcggcg cgggcggttt gcgcgctcaa ctgcttggcc
    16261 agtgcctcgt tggccaggca gcggtcggcg cgggggcgct ctatgccgcg gctttccatg
    16321 atgtcgtaga agtgcaggtc gctggcgatg gcgcgggtgc gctgggccag cgggccgttt
    16381 tcccagcgct ggcgctgggc gggggtggcc gattgggcgc gggccatcca ttcatcctgc
    16441 ctggccagaa agctctggtg cagggcgaag aaggccgggc cgctggcgca atgggtgagc
    16501 agcgccacgg tgagatcgag cggatcacgc aggaaatggc gcacttccag cgcggccttg
    16561 cccggggcaa tggcgcccag gcgcaagggc gggccgccct ccacctcgaa atgggcgcaa
    16621 tgggggcagg tatagctcat atattcggtg atccgccact gggcgtgcgg attgcccagc
    16681 accatggcgc catcggccgt ggccgacact tgcgccagcc agccaccggg cggcggcggc
    16741 accgggcggg caggggcggc atgatgggcg ggcgcgggct gggccagggc ggcgggcgcc
    16801 aggccggcgg ccatggccag ggcaagaaga acgcgcaaca ggggcatcag gccttttttc
    16861 ctttcgccgg gggggcttgt tcccgtccgg ccgaggcatt gcccgccgcg aggctgcgcg
    16921 caagcgattc cagcacggcg cgcagttccg ggtcgccaat gtcgcgcagc gaatcgccca
    16981 gttccaccgg caccggccgc aaggcgggcg gctgggccgg gcgggcgggg ctgggcggct
    17041 ctgccacctc gccctggcgc aggcggatgc gggccaccgc gttatagccg aaaaagcggt
    17101 tcacccgggc gatgatctcg ctttcggcat gggcgatcag cggcgcatgg ccggggcgca
    17161 ccaccagttg cagcgtgccc tcgcagcgct cgccggcggg aaagcggatg gcctcgggcg
    17221 cgcagacgcg ggcctgggcc gccccgacga tctctggcca gcgggtgacg atgctggatt
    17281 gcacgaagcc gaagcggcga aaggcgctgc ggccgatatc gggcatgaga tcggcaatgg
    17341 cgcgggccgg gccgccgcgc gggcgttcat aggggcgctg aggggccttt tttgcctttc
    17401 ccgcgctggc tttgcccgcc cccttcgtgc ccgccgcctt ggctttgcgg ccggcaggtt
    17461 tgggcccctc tggcggggcg gggggcgaag aggcgggagg ctccggttcc attgtgccgc
    17521 cagccatgcc atagccgccc ggtggattcc agagccgacc tcgcccttgc cccccgcacc
    17581 gatccggccg ccctgttgct ggcctggtat gatgcccatg cccgcagcct gccctggcgc
    17641 gcgccgccgg gggcgccgcc catggatgcc tatcgcgtgt ggctttccga ggtgatgctg
    17701 cagcagacca cggtggcggc cgtcatcccc tatttccagc gcttcaccca ggcctggccg
    17761 gatgtgctgg cgctggccgc cgcgcccgag gaagcggtga tggccgcctg ggcggggctg
    17821 ggctattaca gccgggcgcg caagctggtg gaatgcgcaa gggtagtggc ggccaatggc
    17881 ggtgccttcc cccaggagga ggaggccttg ctggcgctgc ccggccttgg gccctatacg
    17941 gccgcggccg tggcggcgat tgcctttggc cggcgggcgg tggtggtgga tgccaatgtg
    18001 gagcgggtgg tggcccgcta tggcgccatt ggcgcgccgc tgccggcggc aaaaccggcc
    18061 atccgcgccg ctaccgacgc gctgacgcct gccgaacgcc ccggcgattt tgcccaggcg
    18121 atgatggatc tgggcgccag cctgtgcagc gtgcgcgcgc ccaaatgcct gctgtgcccg
    18181 ctggccgggg gctgtgcggc gcgcgcggcc ggcaccgccg aggcctatcc ggtgaagccg
    18241 cccaaaaagc ccaagcccca gcgccgtggc cgcgccttct ggatcacccg cgaaggggcg
    18301 gcaggggccg aggtgtggct ggtgcgccgg ccgggcaggg ggcttttggg cggcatgcgc
    18361 gccctgcccg acaatggctg gaacgctcgc gccgatggcg ccggcccgcc gccgctggcg
    18421 ggcccctggc aggccgggcc cagcgtcgct catgtcttca cccatttcgc cctgagcctt
    18481 gaagtggcgt tctattcggg caaggaagcg gccagcctgg ccacaggggc gggcgaatgg
    18541 tggccgctgg cacggctgga cgaggcggga ctgcccagcc tgtttgcccg ggcggccatg
    18601 gcggcgcggg caatgcgcgc ctgaggggga aggagagacc atgcacagaa gcgatgaaca
    18661 gggcgggccg atctgtcggc caggccgccc cggctggagg atctgccatg cgtgataccc
    18721 atgcgccgcg cgccgtggcc tttgccggcg gcgtgctcga cagggccgat ccggtgcggg
    18781 ccgatcccgc cgcgctggca gcgttggccg cccatggccg gctgctgctg ctcgacgggc
    18841 tggagccgct gctgggcgag gggggcgcct tgtgttcggc cccggtgggc cccgatgccg
    18901 gcgagctgat cttccttggc cgcgacgagg ccggcatcgg ctggttcatc gccgcgccag
    18961 acgcggccag cgccagcacc gcgccgccca accccgccct gtgggaagcg atcgccagca
    19021 tggcgccggc tgatctggcg ctgtatggca cggcgcgcag cctggccggc tggcatgccc
    19081 gccaccgctt ctgcgcgcgc tgcggggcgc ccacgcggct ggccaagggc ggctggcagc
    19141 gcagctgcgc cagcgaggcg tgcgctgccg atcatttccc ccgcaccgat ccggtggtga
    19201 tcatgagtgt ggcgcatgag gggcgcctgt tgctgggccg ccagccgcgc tttccgcccc
    19261 atcgctattc ggcgctggcc ggctttatcg agccgggcga ggcggtggag gaggccgtgg
    19321 cgcgcgagat ctgggaagag gcggggctgc gcgtggccga tgtgcgctat gtggccagcc
    19381 agccctggcc gtttccctcc tcgctgatgc tggcctgtca tgccgaggcg ctggacgatg
    19441 ccatcaccat cgacaccacc gaactggaag atgcccgctg gttcacgcgc gccgaagtgg
    19501 ccgaagcgat ggcagcgcgc gcccgtgggg ccgatgggca ggcgttttcc gcgccgccgc
    19561 gccatgccgt ggcctggcat ctgctggccc attggctgga gcagggcgcg gggtaaacgc
    19621 ctgttaacca caggtggcaa ggccaggata atcgcatttt gcgaaactgg tggccgtgat
    19681 ggttcccaag ggcagcaggc ccgccgggga gcctgaaagg accgccatga ctgcctccgg
    19741 cctcaaatcc aggccttctg tccggaccgc tgcccggcca cgcccggccg cgcgcgcgct
    19801 tgccgcagcg ccagcgctgg cggtgccggc ggccctgccg cgcccgcctc aggcgcccgc
    19861 acgcggcacg ctggaggcct tgctgggcct gctggcatgg tggccgctgc cgctgctgct
    19921 ggcggcgatt gtcgcggtgc tggcatggca gggctgggag atgatgcgct ttcgttcgat
    19981 ggcggtgccc ggcggcattg ccgtgcagac agagggcacc cgcgcgacga tcaccggcgg
    20041 gcgggtgtat gtggagttgc cagtgctggt cagcaacagc accgattcgc cgatccgcga
    20101 ggtggccttg tgggcgcggg tctatgcctg cccttcggct ggcgcgcagg tggccgattg
    20161 ccgcctgctg ctctcggccg agcaggacc
//
LOCUS       DASRYE010000063         8512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_3661,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000063 DASRYE010000000
VERSION     DASRYE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VFF98_03425"
     CDS             <1..1537
                     /locus_tag="VFF98_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU62711.1"
                     /translation="ARRPALAALALGTLLAGCTAGPDYHREVPPAPAAWHASLPPAEA
                     HATTDALRPDWWNLYGDPVLSALVSDVARANLDLKIASVRFQQSTAERTIARAARLPQ
                     VNLDASYARERASPNGVMSLLGTSVNQSSGTIASGQNGFGPVGLTSTTSQSQAFDLPQ
                     YGVSASWEVDLWGHVRREMEVANANLRAAEDDRRAVLVSLMAEAAQDYIALRAVQAQL
                     ALTRDHLALARRSVALTGLRRAEGAASKLDEAEANGLLHGYEARLPALEDEETHLVNA
                     LSFLVGREPGALRAQLASPRAIPPVPAQLPVGLPSDLARRRPDVRAAEEKLHAATASI
                     GVAVAEFYPRLTLSGSLDMQALQWGDLASWSSRQYGMGPAISFPLFQGGRLKGQVRLR
                     RAQQKEAAITLQRTLLKAWQEIDDAMADLASAQRARERLEAAVGENRVALDVAQKQYA
                     QGAGDFLHVLTMQNALLASQSAEVNASANVSTAAARLYRALGGGWEATYPEAAGSPRP
                     KAG"
     gene            complement(1581..2810)
                     /locus_tag="VFF98_03430"
     CDS             complement(1581..2810)
                     /locus_tag="VFF98_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HZU62712.1"
                     /translation="MSKPLGPIPTGYGQVGGVLAVDGLPVSEIVRQAGATPLFAYSSV
                     MLRARVAQLRAAMPAQLAIHYALKANPFAPLLRLMAGLVDGFDIASGGELAMAREAGM
                     AAAHTSFAGPGKRDIELQAAIMAGVTLNLESEGEADRALAVAERLGRTPRLAIRVNPD
                     FDLKGSGMKMGGGAKPFGLDAARVPALARRIAATGAEWRGFHIFAGSQALDAGAIIDT
                     QAQTLALAARLGEAAGLAVPHVNLGGGFGIPYFPGDRPLDIAAVGAALGEGLAHLPDA
                     LAGTAFCIELGRWLVGEAGVYLTRILDRKESHGEVFLVTDGGLHHQLAASGNFGTVVR
                     RNYPVAIASHFAAAPTEEASIVGCLCTPLDRLADKADFPRADVGDLVAVFCAGAYGAS
                     ASPAAFLGQGPAREILL"
     gene            complement(2807..4351)
                     /locus_tag="VFF98_03435"
     CDS             complement(2807..4351)
                     /locus_tag="VFF98_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HZU62713.1"
                     /translation="MPLFASPACPAPRPLDHLAHRGEGTAPALILRDFVLSHQTLKER
                     VSRLAGWLAAQVPQRQARVASWLAKGELACLLPLAAARAGLVHVPVNPLLKRAQVAHI
                     LADSGAALLLATPARAATLAPGDVPEGCTLLDEAAALAGAQAATGLPASAADPGDLAA
                     ILYTSGSTGRPKGVMLSHANLWLGAESVATYLELTACDRLLAVLPLSFDYGQNQLLSA
                     WYAGAAVAPLDYLMPRDVVKAVARHGITTLAAVPPLWVQLAEASWPAESIAPLRRLTN
                     SGGALTEPLVRALRALLPQARLYAMYGLTEAFRSTFLAPELIDSHPTSMGRAIPHAEV
                     LVVNAAGAIAADDEEGELVHCGPLVAQGYWHDAARTAERFRPAPAASTYGGMAVWSGD
                     RVRRDGDGLLYFVGRRDAMIKSAGNRISPQEVEEAALATGLVVEAVALGLADARLGQA
                     VHLVARAVAGREAEAEAELPRRLKQDLPNFMQPAAIHWRTALPIGPNGKIDRTGLAED
                     LNGASV"
     gene            4422..5384
                     /locus_tag="VFF98_03440"
     CDS             4422..5384
                     /locus_tag="VFF98_03440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU62714.1"
                     /translation="MQEQPALAALLAAADAPFDRVAWWQGLVADCGLSPLYASAQVPG
                     GGAALMALQQVEGGLAALVNWYSFRWRVLLGDAALGPALLRALAGGLAGVAGRLTLAP
                     LDEDEAEALAIALRQTGWWVEQSFLHHNHILRPAGRSGAAFLAGRPGAMRSTIRRMGA
                     RMETRIHRAFDADAWAAYEAIYADSWKPVEGSPAFLRHFAEAEGAAGRLRLGLALLEG
                     EPVAAQLWTVEAGTAYIHKLAYRESARRFSPGTVLSAAMFTAAIDEDRVALIDYGTGS
                     EPYKADWTEEARPRYRLTAWRPARPANWPPIARALARRLIRRYP"
     gene            5507..5773
                     /locus_tag="VFF98_03445"
     CDS             5507..5773
                     /locus_tag="VFF98_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU62715.1"
                     /translation="MPEQTDHLLRTILADVLGLPQPVVAGFDEDTGLFGHLPELDSMA
                     VAGLLTEIEDRFGIVIEDDEVDGELLATFGALVAFVTAKRAEAA"
     gene            5778..6446
                     /locus_tag="VFF98_03450"
     CDS             5778..6446
                     /locus_tag="VFF98_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62716.1"
                     /translation="MHAGATRIASWPAPAGGAEMAVLCGPEQAAPLLLLPALFDEGNK
                     LRRFTLDLMRALAGHGIASVLPDLPGSNESLAPLEAQDLGLWRQAMAAAAAHFGAIRG
                     LALRGGALVAPAGLAGRAYAPVAGASLLRQMLRARIIAAHEAGLEESTDALLATGQSQ
                     GLELAGHRLGPRMLADLAAAQPAPALTPIAPGDIPGPGLWLRAEPGENRAQAEALAAV
                     VAGA"
     gene            6443..7234
                     /locus_tag="VFF98_03455"
     CDS             6443..7234
                     /locus_tag="VFF98_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HZU62717.1"
                     /translation="MIRRHFTIPCAGAQLAATLDLPASGAPTSALLLVSGGNEIRAGA
                     FAGQARLAARITAAGHAVLRFDRRGVGDSSGENAGFRHSAEDIAAALAALRAAVPGLC
                     YIVAFGNCDGASALMLAGGAGVNALVLANPWTIEQEDEAAPAPPAALRAHYRARLANP
                     AAIRRLLTGQVAPMALLRSLAAMARPRAPSTNSLAAAMAEGLARFTGPVRILLAGRDR
                     TAQAFLGLWNRKDQRLAHCPGASHAFVEAEAREWLLEQILAALAP"
     gene            complement(7270..8232)
                     /gene="trxB"
                     /locus_tag="VFF98_03460"
     CDS             complement(7270..8232)
                     /gene="trxB"
                     /locus_tag="VFF98_03460"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZU62718.1"
                     /translation="MTTHTTRMLIIGSGPAGLTAAIYGARAGLSPIVVQGLQPGGQLT
                     ITTDVENYPGFRETVQGPWLVQEMQAQAEHVGTRMMWDTIVDIDLAASPFRAVGDSGD
                     VYEGDTLVICTGAQARWLGVEGEQALSGKGVSACATCDGFFYRGKKVVVIGGGNTAVE
                     EALYLTNHSQDVTLIHRRDSLRAEKILQDRLFAHPHIKVLWNKAVERFEGEGGIGGAG
                     LTGVALVDTVTGEKSVEPAQGAFVAIGHAPATELFKGKLELDESGYIIVQPGTPKTAI
                     PGVFAAGDVMDHTYRQAVTAAGTGCMAALDAERFVGELDFARAQ"
BASE COUNT         1260 a         3101 c         2924 g         1227 t
ORIGIN      
        1 ggcccggcgc cccgctcttg ccgcgctggc gctgggcacg ctgctggccg gctgcacggc
       61 agggccagac tatcaccgcg aagtgccccc cgccccggcc gcctggcacg ccagcctgcc
      121 ccccgccgag gcccatgcca cgaccgacgc cctgcggccc gactggtgga acctctatgg
      181 cgatccggtg ctgagcgcgc tggtgagcga tgtggcgcgc gccaatctcg atctcaagat
      241 cgcctcggtg cgcttccagc aaagcaccgc cgagcgcacc attgcccgcg ccgcccgcct
      301 gccgcaggtg aacctcgatg cctcctatgc ccgcgagcgc gccagcccca acggggtgat
      361 gagcctgctg ggcacctcgg tcaaccagag cagcggcacc attgccagcg gccagaacgg
      421 ctttggccct gtcggcctca ccagcaccac cagccagtcc caggctttcg atctgccgca
      481 atatggcgtc tcggcctcgt gggaggtgga tctgtggggc catgtgcggc gcgagatgga
      541 agtggccaat gccaatctgc gcgcggcaga ggatgaccgg cgcgcggtgc tcgtctcgct
      601 gatggccgag gccgcgcagg attacatcgc cctgcgcgcg gtgcaggccc agctggcgct
      661 gacgcgtgac catctggcgc tcgcccggcg cagcgtggcg ttgaccggcc tgcggcgcgc
      721 agaaggcgcg gccagcaagc tggatgaggc cgaggccaat ggtctgctgc atggctatga
      781 agcgcgcctg ccggcgctgg aggatgagga gacccacctt gtcaacgcgc tcagcttcct
      841 ggtcgggcgg gagccggggg ccttgcgcgc gcagctggcc agcccgcggg ccattccccc
      901 ggtgccggcg caattgccgg tgggcctgcc ttccgatctg gcccggcggc gccccgatgt
      961 gcgcgccgcc gaggagaagc tgcatgccgc caccgccagc attggcgttg ccgtcgccga
     1021 attctacccg cggctgacgc tgtcgggcag cctcgacatg caggcgctgc aatggggcga
     1081 tctggccagc tggtcctcgc ggcaatatgg catggggccg gcgatcagct ttccgctgtt
     1141 tcagggcggg cggctgaagg ggcaggtgcg cctgcgccgc gcccagcaga aggaagccgc
     1201 catcaccctc cagcgcacgc tgctgaaggc ctggcaggag atcgacgatg cgatggccga
     1261 tctcgcctcg gcgcaaaggg cgcgcgagcg gctggaggcg gcagtgggtg agaaccgcgt
     1321 ggcgctggat gtggcgcaga agcaatatgc ccagggcgcc ggcgatttcc tccatgtgct
     1381 gaccatgcag aacgccctgc tggccagcca aagcgccgag gtgaacgcca gcgccaatgt
     1441 ctccaccgcc gctgcccgcc tctaccgagc gctgggcggc ggctgggagg ccacctatcc
     1501 cgaagcagcc ggcagccccc gtcccaaggc cggctgatgc gggggcccgg gcgctctccc
     1561 ggccgcccgg gccaaccgtt ttacagcagg atctcgcgcg cggggccctg ccccagaaaa
     1621 gccgccgggc tggcgctggc gccataggcc ccggcgcaga acaccgcgac cagatcgccc
     1681 acatcggcac gggggaaatc agccttgtcg gccagccggt cgagcggggt gcacaggcag
     1741 ccgacgatgc tcgcctcctc tgtgggcgcg gcggcgaaat ggctggcgat ggccaccgga
     1801 tagttgcgcc gcaccaccgt gccgaaattg ccggaagcgg ccagctggtg atgcaggccg
     1861 ccatcggtga cgaggaacac ctcgccatgg ctttccttgc gatcgagaat gcgggtgaga
     1921 taaacccccg cctcgcccac cagccagcgg cccagctcga tgcagaaggc ggtgcccgcc
     1981 agcgcatcgg gcagatgggc caggccctcg cccagcgctg cgcccacagc ggcaatgtcg
     2041 agcggcctgt cccccgggaa ataggggatg ccaaagccgc cgcccaggtt cacatgcggc
     2101 acggccagcc ccgccgcctc gcccagccgg gcggccagcg ccagggtttg cgcctgcgtg
     2161 tcgataatgg cgccggcatc cagcgcctgg ctgccggcaa agatgtgaaa gccgcgccat
     2221 tcggcccctg tcgcggcgat gcgccgggcc agcgccggca cgcgcgccgc atccaggcca
     2281 aacggcttgg cgccgccgcc catcttcatg ccgctgccct tgagatcgaa atcggggttc
     2341 acgcggatgg caaggcgcgg cgtgcgaccc agcctttcgg ccaccgccag cgcccggtcc
     2401 gcctcgccct cgctttcgag gttgagagtg acgccggcca tgatcgccgc ctgcagctcg
     2461 atatcgcgct tgccggggcc ggcaaagctg gtgtgcgcgg ccgccatgcc ggcctcgcgg
     2521 gccatcgcca gttcaccgcc actggcaatg tcgaaaccat cgaccagccc ggccatcagc
     2581 cgcagcagcg gcgcaaaggg attggccttc agcgcataat ggatcgccag ctgagccggc
     2641 atggccgcgc gcaactgcgc cacacgcgcg cgcagcatca ccgaggaata ggcaaacagc
     2701 ggggtggcgc cggcctgccg cacgatctcg ctgaccggca ggccatccac cgccagcacg
     2761 ccgcccacct ggccatagcc tgtgggaatg gggcccaggg gtttgctcat accgatgctc
     2821 cgttcaggtc ttcggcaagg ccggtgcggt cgatcttgcc attggggccg atgggcagcg
     2881 cggtgcgcca gtgaatggcc gccggctgca tgaaattggg caggtcctgc ttcagccggc
     2941 ggggcagctc ggcttccgct tcggcttccc ggccggccac cgcgcgcgcc accagatgca
     3001 ccgcctgccc cagccgcgca tcggccaggc ccagcgccac cgcctcaacc acaaggccgg
     3061 tggccagcgc tgcctcctcc acttcctgcg ggctgatgcg attgccggcc gatttgatca
     3121 tcgcatcgcg ccggcccacg aaatagagca gcccatcgcc atcgcgccgc accctgtcgc
     3181 cgctccacac cgccatgccg ccataggtgg aagccgcagg cgccgggcga aaacgctcgg
     3241 ccgtgcgtgc ggcatcgtgc caatagccct gcgccaccag cggcccgcaa tgcaccagct
     3301 cgccctcctc atcatccgcg gcaatggcgc cggccgcgtt caccaccagc acttcggcat
     3361 gggggatggc gcggcccatg ctggtggggt ggctgtcgat cagctctggc gccaggaagg
     3421 tggagcggaa ggcctccgtc agcccataca tggcatagag gcgcgcctgc ggcaaaagcg
     3481 cgcgcagggc ccgcaccagc ggctcggtca gggcgccgcc gctgttggtc agccggcgca
     3541 aaggggcgat gctttcggcc ggccagctgg cctcggccag ttgcacccac agcggcggca
     3601 cggcggccag cgtggtgatg ccatggcgcg ccaccgcctt caccacatcg cgcggcatca
     3661 gataatcgag cggggccacc gccgccccgg cataccaggc cgacagcagc tggttctgcc
     3721 cataatcgaa gctgagcggc agcaccgcca gcagccggtc acaggccgtc agttcgagat
     3781 aggtggccac gctttcggcc cccagccaca ggttggcatg gctgagcatc acccccttgg
     3841 gccgcccggt ggagccgctg gtgtagagga tggcggccag atcgcccgga tctgcagcgc
     3901 ttgcgggcag gccggtggcc gcctgtgccc cggccagcgc tgcggcttcg tccagcaggg
     3961 tgcagccttc gggcacatcg ccgggggcaa gggtggccgc gcgcgccggc gttgccagca
     4021 gcagggccgc gccgctgtcg gccaggatat gcgccacctg cgcgcgcttc agcagcgggt
     4081 tcaccggcac atgcaccagc cccgcgcgcg cggccgccag cggcagcagg caggccagct
     4141 cgcccttggc cagccagctg gccacccgcg cctgccgctg cggcacctgc gccgccagcc
     4201 agcccgccag ccggctgaca cgctctttta atgtctggtg gctaagcaca aaatcgcgca
     4261 ggatcagggc cggcgcggtg ccctctcccc tgtgcgcaag atgatccagc ggccgggggg
     4321 ccgggcaagc ggggctggcg aacaggggca tggcgataag ggcgatcaat ccggtgaagg
     4381 cgttggggct aaggctggca tatcacgccg atcttgccca agtgcaagag cagccggcgc
     4441 tggccgccct gctggccgcc gccgatgccc cgttcgaccg cgtcgcctgg tggcaggggc
     4501 tggtggcaga ttgtggcctc agcccgctct atgcctcggc gcaggtgccc ggcggcggcg
     4561 cggcgctgat ggccctgcag caggtggaag gcgggctagc ggcgctggtc aactggtaca
     4621 gtttccgctg gcgggtgctg ctgggcgatg cagcgctggg cccggcgctg ctgcgcgcgc
     4681 tggccggggg cctggccggc gttgccgggc gcctcaccct tgccccgctg gacgaggacg
     4741 aggccgaagc gctggccatc gccctgcggc agacaggctg gtgggtggag caaagcttcc
     4801 tgcaccacaa ccacatcctg cgccccgccg gccgcagcgg cgccgctttc ctcgccggcc
     4861 ggccaggggc catgcgcagc accatccgcc gcatgggcgc gcgcatggaa acccgcatcc
     4921 accgcgcctt cgatgccgat gcctgggccg cctatgaagc gatctatgcc gacagctgga
     4981 agcctgtgga aggatcgccc gccttcctgc gccatttcgc cgaggcggaa ggggccgccg
     5041 gccggctgcg gctgggcctg gccctgctgg agggcgagcc cgtggccgcc cagctgtgga
     5101 cggtggaagc cggcaccgcc tatatccaca agctggccta ccgcgagagc gccaggcgct
     5161 tttcccccgg cacggtgctg agcgcggcca tgttcaccgc cgcgatcgac gaggaccgcg
     5221 tggcactgat cgattacggc accggctccg agccctacaa ggccgactgg accgaggagg
     5281 cgcggccgcg ctatcgcctg accgcatggc ggcccgcccg gccggccaac tggccgccga
     5341 tcgcccgcgc gctggcccgc cgcctgatcc gccgctaccc atgagcacgc gcgtggatga
     5401 cgccttgcgc ccgcccgccg agggggctag agcccggcac aaatctgcgg ccgttgccgg
     5461 ggcatccctg cccgccggcc tttgccaaca taaagtcagg ggagccatgc cagagcaaac
     5521 cgatcacctg cttcgcacga tcctcgccga cgtgctgggc ctgccgcagc cggtcgtcgc
     5581 cggatttgac gaggataccg gcctgttcgg ccatctgccc gagctggatt ccatggccgt
     5641 cgctgggctg ctgaccgaaa tcgaggaccg cttcggcatt gtcatcgagg atgacgaggt
     5701 ggatggcgaa ttgctggcca ctttcggcgc gctggtggct ttcgtaacgg caaaacgcgc
     5761 cgaagctgcc tgagcccatg cacgccggcg ccacgcgcat cgccagctgg ccggcccctg
     5821 cgggcggcgc ggaaatggcg gtgctgtgcg ggcccgaaca ggctgccccg ctactgctgc
     5881 tgccggccct gttcgacgag ggcaacaagc tcaggcgttt cacgctggat ctgatgcgcg
     5941 cgctggcggg ccatggcatt gccagcgtgc tgcccgatct gcccggcagc aacgaaagcc
     6001 tcgccccgct ggaagcgcag gatctgggcc tgtggcgcca ggcgatggcg gcagcggcgg
     6061 cccatttcgg cgccatccgg gggctggccc tgcgcggcgg cgcgctggtg gcgcccgcag
     6121 gccttgccgg ccgggcctat gcgccggtgg ccggcgccag cctgctgcgc cagatgctgc
     6181 gcgcccgcat catcgccgcg cacgaggccg ggctggagga gagcacggac gcgctgctgg
     6241 ccaccggcca atcgcaaggg ctggaactgg ctggccaccg gctgggcccg cgcatgctgg
     6301 cggatctggc cgctgcccag cctgcccccg ccctcacgcc catagcgccc ggagacatcc
     6361 ccggccccgg cctgtggctg cgggcagagc ccggcgagaa ccgcgcccag gcagaagcgc
     6421 tggccgctgt ggtggccggc gcatgatccg ccgccatttc accatccctt gcgccggcgc
     6481 gcaactggcc gccacgctgg acctgcccgc cagcggcgcc cccaccagcg ccctgctgct
     6541 cgtctcgggc ggcaacgaga tccgcgcggg cgcctttgcc gggcaggccc ggctggccgc
     6601 ccgcatcacg gccgccggcc acgccgtgct gcgcttcgac cggcgcggcg tgggcgacag
     6661 cagcggcgag aatgccggct ttcgccacag cgccgaggat atcgccgctg cccttgccgc
     6721 cctgcgcgcg gccgtgccgg gcctttgcta cattgtcgct ttcggcaatt gtgatggggc
     6781 cagcgcgctg atgctggccg gcggcgcggg cgtgaacgcg ctggtgctgg ccaacccctg
     6841 gacgatcgag caggaggacg aggccgcccc cgcgcccccg gcggccctgc gcgcccatta
     6901 tcgcgcgcgc ctggccaatc cggcggcgat ccggcggctg ctcaccgggc aggtggcgcc
     6961 gatggcactg ctgcgcagcc ttgccgccat ggcccgcccg cgcgcgcctt ccaccaactc
     7021 gctggccgcc gccatggccg aggggctggc gcgtttcacc ggcccggtgc gcatcctgct
     7081 ggccgggcgc gatcgcaccg cgcaggcctt ccttggcctc tggaaccgca aggatcagcg
     7141 cctggcccat tgccccggcg ccagccacgc ctttgtggag gccgaggcgc gcgaatggct
     7201 gctcgagcag attctggccg cattggcgcc atgaggaagc gaggggatgg cccctcgccc
     7261 cattccttct tactgcgcgc gggcgaaatc cagttcgccc acaaagcgtt cggcatccag
     7321 cgcggccatg cagccggtgc cggccgccgt caccgcctgg cgataggtgt ggtccatcac
     7381 gtcgccggcg gcaaacacgc cggggatcgc cgtcttgggc gtgccgggct gaacgatgat
     7441 gtagccgctc tcgtccagct ccagcttgcc cttgaacagc tctgtcgccg gcgcatggcc
     7501 gatcgccacg aaagcgccct gggccggctc cacgctcttc tcgccggtca ccgtgtccac
     7561 cagcgccacg ccggtcagcc cggcgccgcc gatcccgccc tcgccctcga aacgctccac
     7621 cgccttgttc cacagcacct tgatgtgggg gtgggcaaag aggcggtcct gcaggatctt
     7681 ttccgcccgc agcgaatcgc gccggtggat cagcgtgaca tcctgcgagt gattggtgag
     7741 gtagagcgcc tcttccaccg ccgtgttgcc accgccgatg accaccacct tcttgccgcg
     7801 atagaagaag ccatcgcagg tggcgcaggc cgaaacgccc ttgcccgaca gcgcctgctc
     7861 gccttccacg cccagccacc tggcctgggc gccggtgcag atcaccagcg tgtcgccttc
     7921 atagacatcg cccgaatcgc ccactgcccg gaacgggctg gccgcaaggt cgatatcgac
     7981 gatcgtgtcc cacatcatgc gcgtgcccac atgttctgcc tgggcctgca tttcctgcac
     8041 cagccagggg ccttgcaccg tctcgcgaaa gcctggataa ttctcgacat cggtggtgat
     8101 ggtcagctgc ccgcccggct gcaacccctg cacgacaatg ggcgaaagcc ccgcgcgcgc
     8161 gccataaatc gccgccgtga ggcctgcggg cccggaaccg atgataagca tgcgggtggt
     8221 gtgggtggtc atgtgtggca tatctccgtt gccggggcga gataggcaag cccggccgcg
     8281 aaagccagag gcaagcgggc atctggcgcc aaaagcgggc caaagaaatt ctcgtggcgg
     8341 ggcaattgcc gctacaggtt cacccggcgg taaggttggc catgttaaac cccaatcagc
     8401 gagtaggaat agtaaggcag agcgtgggca gggtatgttt gcggtcgctc ccataagggc
     8461 aactgccatg ccctgctcct ttttggcttg cggcctgctg tggccggcgg cc
//
LOCUS       DASRYE010000064        24833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_74512,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000064 DASRYE010000000
VERSION     DASRYE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 24833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24833
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74512"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFF98_03465"
     CDS             complement(<1..652)
                     /locus_tag="VFF98_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU62719.1"
                     /translation="MRKIIIGLALASSALATPALARDKSWYVEGDAGGVIAEDQTVTS
                     TGSYSGTAAFKAGYDFGGIVGYDFGPFRLESEASYRRVALSKLTTSGGTTYSNSTGLS
                     GSADALSFMLNGLLDFGPDNGLQGFVGGGVGVARVQERGLQSGYGLDDSATGFAWQLL
                     AGIRVPVSKNLDAGLKYRFFRGEGADNFVATDGSGVNARFQSHSLLATLTYNFGAPT"
     gene            complement(807..1265)
                     /locus_tag="VFF98_03470"
     CDS             complement(807..1265)
                     /locus_tag="VFF98_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HZU62720.1"
                     /translation="MSDTISFDSTSPRFGLPLLFAAQSQKEVYVNEALALVDGLIHCA
                     IEGSATTPPSAPADGTAWLVGTGASGDWAGEDGKLACHQAGAWIFVAPCNGMNLLNKA
                     NGQFTHYLNGWQSPALVAAPSGGTVIDSQARTAIAALISALQAAAILPSG"
     gene            complement(1285..3465)
                     /locus_tag="VFF98_03475"
     CDS             complement(1285..3465)
                     /locus_tag="VFF98_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZU62721.1"
                     /translation="MATLVFTALGTLVGGPLGGVLGAMAGREIDNSLFGTDVQGPRLK
                     DLSVSTSTYGTAIARHFGQMRSAGSIIWATDLVEHASTSGGGKSSVSVTTYSYTSSFA
                     VAVASRQILSIGRIWADGQLLRGAAGDLKVGVTLRIYDGSQDQAADPLIAANEGAANC
                     PAFRGIAYVVFEDLQLADYGNRIPSLTFEVFADSGTLTLASLFEGWLEDVDADVPLTG
                     IAGYSSDGAFNATLSQFQPVFPMDCDAGGSGLTLSRQRLQSAPIALGEPAVAAGNQGN
                     FGGKHGFSRKLAPVTENPPRILRYYDIALDYQPGSQRAPGQPLPGQPKSIDLPAAITA
                     LNAFGLIAQTARNTNWARQTIAWRCAQLDPAVAPGAVVSLPDKAGQWRVTEWEWRDTG
                     IELALERLAPTAGTSAAVYAGAASLAADVAVGASALFAYELPWDGTGSNAQTLIQAAV
                     SSASSGWAGAAILVDDEAGALTQISASGRLRSTIGTTQGALPAASPLFFDRASTLDVQ
                     LLASDMALAGATARQLVTGANRALVGAEIIQFAGAAALGGGVWQLSGLLRGRGGTEAA
                     IAGHAAGEPFVLLDSLPVTLDSSQIGADARIAALGLGDASAVEADIVNRGIGLKPLAP
                     VHPNAVANADGSLTLGWTRRARGAWLWLDGVDVALQEESEAYIVCYGPLAAPLAMWST
                     PAAQLNLGASTLATLRGQLANGPFYVRQQGTSGLSTALFLATLS"
     gene            complement(3520..3930)
                     /locus_tag="VFF98_03480"
     CDS             complement(3520..3930)
                     /locus_tag="VFF98_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62722.1"
                     /translation="MPAEAAGAQVAARARALVGAPFRLHGRDPASGLDCVGLVALAIG
                     AGGGAPVAYRLRMREIGPLLAQAAHCGLRPATGAVLPGDVLLLAVGPCQFHLAVAVEG
                     GACVHAHAGLRRVVISPALPEGRRLRHWRLAPDA"
     gene            complement(3930..4745)
                     /locus_tag="VFF98_03485"
     CDS             complement(3930..4745)
                     /locus_tag="VFF98_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HZU62723.1"
                     /translation="MSRVWFSQGLEAVATYWRIARTDGVALGFTTHDADLWFDGVLHQ
                     ACPGMIPAAIRRSAAFDSDSAEVTGAITHAALNGEDLVSGRFDNARVIVGLVDWETLE
                     HQPIYSGSLGQVSAEDGTFVGELLSRKAELARDIVPRTSPTCRADFCGTGCALSAVSY
                     THEASVTAYDSTANAVTLSSDVAAAHLVSGALRWLDGPYAGMAMGIVDAAGSTAVLDT
                     PLDRPLAGVVRVLLLEGCDRTLATCAGRFGNTLNFQGEPFLPGNDLILRYGLA"
     gene            complement(4742..7078)
                     /locus_tag="VFF98_03490"
     CDS             complement(4742..7078)
                     /locus_tag="VFF98_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HZU62724.1"
                     /translation="MACWLASAIDGQETGWIQRFDPRFWTVNFPRPMMAALTNPAADA
                     IRVDATFLTAADLAGLVWESADSLDHPLLAYATNSDYTGATLSFTWTSGGIIALDQVN
                     GPTLTIEGLDASGAAHTWYVRLWNYASGTPTDAAISLPFSALVGGWATSGAGADPVWP
                     AAISRMFISLAAPGYSGAATPLAAAVDGWAQLAGLRCAGAGAMLKIGNVFVPPGGGAI
                     ATSFDDQGNQSPARLVRGLRQLGYRGSVLHYVGMSHHTRLADVGGAYLVGQGGDPLNA
                     PARAWHAEFFQRCGAAGLSPIASLSFEVLAQYCPAGWQQMDSAGNPALTAWEPPSTLL
                     SPANAAAMGWLQQVAAAYAGLLQAAGVAVRVQIGEPWWWITASHGICLYDAAAAAALG
                     GSPVAIADLTQPLTSAQTALLDAAGALLASATAGLVAAVRAAAGTAGAEVLLLVFTPT
                     VLNPATPEAQRANLPLGWAAPAFDRLQVEDYDWLTAGADGLRRQAYQQVDSRLGYPVA
                     QQDYLAGYVPDATQADLWRRVDAGMAEAFARGVHEVFVWALPQVVRDGFVHMASTEDE
                     EDMQAFDDVVYPLPLGLDAHVSPAFSTTITTTASGFEHRASVWRDARLSFDVGPGVRS
                     EGDLGTLIAFFRARRGAARGFRLADPSDFSSNGMTGVPGPADQALGTGDGATASFALV
                     KAYGEAGCQQIRRITRPRAGTVRVSLGGVEQASGWTPGDGGMITFATAPAAGVAVAAG
                     FCFDVPVRFEQDKLEVTGVTFAASEAPSVPVVEIREAV"
     gene            complement(7092..7697)
                     /locus_tag="VFF98_03495"
     CDS             complement(7092..7697)
                     /locus_tag="VFF98_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HZU62725.1"
                     /translation="MSSSSRYTSSTGSTSQSLTIDVRASTQNFAQDIAAMRSSVDGTL
                     LTGFTQAGSTLDTGLTNALAKGVTGFTNLRTTALGVINDIAAQASSNLFASVGSDTGA
                     VASGGLLGVDLVSSLLTGLPGRATGGAVSPGQAYMVGERGPEVFVPTSAGSIAANGSG
                     AARETRVSINVNAPQGSDGPQALQRSSRQIASAMRRALAGN"
     gene            complement(7684..7884)
                     /locus_tag="VFF98_03500"
     CDS             complement(7684..7884)
                     /locus_tag="VFF98_03500"
                     /inference="COORDINATES: protein motif:HMM:NF021092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HZU62726.1"
                     /translation="MSGFGASARRLAGLSARLLGWRPAEFWAATPAELAAILRPEGAP
                     QGPLPLTRAEMNRLMERDHEQL"
     gene            complement(7881..8192)
                     /locus_tag="VFF98_03505"
     CDS             complement(7881..8192)
                     /locus_tag="VFF98_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gene transfer agent family protein"
                     /protein_id="HZU62727.1"
                     /translation="MSGANPWRGEAKLVLEGAERVLRPSFAALVAAEEELGSLFALVE
                     RAGAGHLRLAEVAALFWHCLADAGEMSREAFSEAVLKLGLARAMAPLRALLEQILQGA
                     A"
     gene            complement(8189..8596)
                     /locus_tag="VFF98_03510"
     CDS             complement(8189..8596)
                     /locus_tag="VFF98_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZU62728.1"
                     /translation="MTAQSGAAFLLKITDGASPPNYQTVAGLRTTQLSIAGQAVVITT
                     KDSNGWRDLLNNGGARSISVSAAGVFMGSAAEGRIRGNALAGTIDAYQLCFEDGATMT
                     GAFLVQKLEYAGDFNNERSYTLQLESSGVVVAA"
     gene            complement(8630..9019)
                     /locus_tag="VFF98_03515"
     CDS             complement(8630..9019)
                     /locus_tag="VFF98_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HZU62729.1"
                     /translation="MELPLRAALIGWLAGDAALAGQLNAITEEGPAFTSLPWLAISAS
                     ASVDWSTKTETGYESRIAFELHVRADQPGNAAPLVAAIQAALAAMPRAQAGFTIIGLQ
                     FMRQRAEQLTETTRTVLLEYRFRTIAG"
     gene            complement(9025..9213)
                     /locus_tag="VFF98_03520"
     CDS             complement(9025..9213)
                     /locus_tag="VFF98_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62730.1"
                     /translation="MISATPQPSLEALSEALTEAAATLATAQAQSRQLEASGSPLRWR
                     TASLVWPLFTASLMKTKG"
     gene            complement(9210..9851)
                     /locus_tag="VFF98_03525"
     CDS             complement(9210..9851)
                     /locus_tag="VFF98_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62731.1"
                     /translation="MMRVIVNPAVLPAEALAELKQWLGITTGQDDAPLAALLAAALDV
                     CADFTGLMPLACGCEEMVPLPADWRHMPRADAWQQRRFPKEWPRHNPFALPAEGWLAL
                     DTRPVLAIAEMALVAADGSRTPLDPALWHERVDAEGAGFVRVDQPGRAMRAVVSFTAG
                     LAPDWDHLPQPLRHGIIRLAAHQHRTRESDGAAPLPPASVAALWRPFRRVRLV"
     gene            complement(9990..11132)
                     /locus_tag="VFF98_03530"
     CDS             complement(9990..11132)
                     /locus_tag="VFF98_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HZU62732.1"
                     /translation="MEHDDTAQALDASFDVVARQEAAEQALGALRGEMAEVKSRIDRL
                     GRAPVRPVIGSAAAGGGGEVKSFVDGYLRAGRDGELKSMVIGTPSQAGYLVPTELDKR
                     IAEVLLRVSPIRQVAQVVQTSSSDYRKLISLGGTASGWVSEAAARPETTEPSFAEIVP
                     PHGELYANPSASQQMLDDALFDLEGWLAGEIAREFGRAEGAAFVNGNGSNQPAGFLAA
                     ATSEAPDGSRPFGTLQYVASGNASGFDTSPDLHLIDMVMALNPGHRQGAVWVMNASTL
                     AQIRKLKDTIGDYLWQPGMMDGQPDRLLGYPVIEAADMPNIAAGASPIAFGNFQAGYL
                     ITERTGTRILRDPYSNKPFVNFYATKRTGGQVLDSNAIKLLTIAAS"
     gene            complement(11214..11642)
                     /locus_tag="VFF98_03535"
     CDS             complement(11214..11642)
                     /locus_tag="VFF98_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HZU62733.1"
                     /translation="MTAPQGDAALRFAGYAALFDKRDAGADVIRPGAFARTLAERRRA
                     GQGPLPLYWQHRPDVRIGWIDEASEDARGLRVTARIDNPQGGAAMALKAGKVTGLSFG
                     YRARGFTRDTAGRDLTDIDLFEVSLVSNPMQAGARVHMIA"
     gene            complement(11639..11944)
                     /locus_tag="VFF98_03540"
     CDS             complement(11639..11944)
                     /locus_tag="VFF98_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HZU62734.1"
                     /translation="MREDMLARLIAQGVEQGSDLVTLRALVEEASELGAGRMLARMGL
                     GDARASDDMSELRQLLAAWREARRGAWRVLGEWLMRAVLALLLVLIAFRSDVTELLK"
     gene            complement(11944..13131)
                     /locus_tag="VFF98_03545"
     CDS             complement(11944..13131)
                     /locus_tag="VFF98_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZU62735.1"
                     /translation="MNLLQSLASAFKGKGPRVPLARGGASPWASPWLFADAGISRVPY
                     EYNCAVMRGYLDNPVAQRAVRLVADGIGGAPLMPADPALLALVKATSAGQALMETLTS
                     QLLLHGNAYVQVLKDAEGRPVELFALRPERVTVMPGADGWPCSLSYQVGDKTMVIPLV
                     DGDASPNVIHIRGYHPTDDHYGAGCLSAADQAIATHNAAANWNRSLLENAARPSGALV
                     YDPGNGGGLSPDQFDRLREELNRAFSGSFNAGRPLLLEGGLKWQSLSMSPADMDFASL
                     KAAAARDIALAFGVPPMLLGLPGDATYANYKEANRALWRLTLLPLSAKLFAALTEGLA
                     TWFPEAQLAIDLDRVPALADDRQALWTQVNAASFLTQAEKRELLGFAAQPAGAAPIVD
                     IPV"
     gene            complement(13203..14528)
                     /locus_tag="VFF98_03550"
     CDS             complement(13203..14528)
                     /locus_tag="VFF98_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HZU62736.1"
                     /translation="MSLDERLAWLLGLPDGERHAALAALTREEQRELAWHWRLWARSE
                     QLPPPGDWRCWLVMAGRGFGKTRAGAEWVRAIAQARPEARIALVGASLGEVRAVMVEG
                     ESGLLAIAPPGRRPRYEPSLRRLVWPNGAQALLYSAAEPESLRGPQHSHGWCDEIAKW
                     DNAGMRAEQTWHNLLLGLRLGEDNRVMATTTPRAVPLLLQLLAREGVAVTRGRSEANA
                     AHLPPRFLADMRRAFGRSQWARQELDGELITEQEGALWSRALLERCRESAAASPPARV
                     VIGVDPPASATGDACGIVVVALGTDGLARLLADATLRRAGPERWARAVAEAAAHWRAD
                     RVVAEANQGGAMVESVLRAAELHLPVRLVHARVGKAARAEPVAALYEAGRVRHVGHFP
                     ELEDQLCGLIAGGAYQGPGRSPDRADALVWALSELMLGPRAAPRVRMDF"
     gene            complement(14515..15018)
                     /locus_tag="VFF98_03555"
     CDS             complement(14515..15018)
                     /locus_tag="VFF98_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62737.1"
                     /translation="MVDRSEPIARVRAVPAGKGVRSQWRRVFLAELAATSNVSAAARK
                     ARVSTTKAYELRRADPAFARAWRAALCEGYDHLEMELLRRLRCGEIKPAAGARRSARA
                     FDNATALRLLTAHRETVARERAVQGEEDADAILASINAKLDRMRERQAGRENGGESES
                     EGGDVAG"
     gene            15228..15878
                     /locus_tag="VFF98_03560"
     CDS             15228..15878
                     /locus_tag="VFF98_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZU62738.1"
                     /translation="MLRRLYDWTMAKAEGPSAPAWLALFAFVEASFFPIPPHPVLGLM
                     CLAQPKKAIRFALITTLASVAGGLLGYAIGHFAFALVGEKLLGLLHLTQSFPYAACKL
                     RGDLGFMAIIAKGATPIPFKLVTITAGFVGMPLGKFLLASFISRSISFLIVGVLFRLF
                     GAPIKAWIDRYLGLAVAGFVVLIIAGFAAAALLGHGGGGKADPCTGATLQSLSASA"
     gene            complement(15953..17179)
                     /locus_tag="VFF98_03565"
     CDS             complement(15953..17179)
                     /locus_tag="VFF98_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU62739.1"
                     /translation="MTETREAPVAADLFAKFDPMITLRRDLLASGVTDPFGLVMEKVI
                     SPTVALCNGRETILLGTYNYMGMTFDADVIAAGKQALDDFGAGTTGSRVLNGTYAGHK
                     AVEEALKEFYGMDHAMVFSTGYQANLGIISTIAGKGDYIILDIDSHASIWDGCKLGDA
                     EVVPFKHNDIEAMEKRLKRVPAGAGKLVVLEGVYSMLGDIAPLKEMIRIAKENGAMVL
                     VDEAHSMGFIGPNGRGVAEEQGVLDDVDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLV
                     CRPYVFTASLPPSVVATAATSIRKLMHAGEKRAHLWENSRTLHKGLREAGFQLGTDEP
                     QSAIISVIMPDLERGAAMWEALLHEGLYVNLARPPATPAGMTLLRCSLCALHTAEQVQ
                     TIIGMFQRAGAKAGIL"
     gene            complement(17188..17427)
                     /locus_tag="VFF98_03570"
     CDS             complement(17188..17427)
                     /locus_tag="VFF98_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU62740.1"
                     /translation="MDRAATQAKIAELIGPFNKKGAPLADATTFAGDLEFDSLTVMDF
                     VAAIEDEFDIIISMSQQAEIETYGQLVDAVVKLKG"
     gene            complement(17537..17905)
                     /locus_tag="VFF98_03575"
     CDS             complement(17537..17905)
                     /locus_tag="VFF98_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU62741.1"
                     /translation="MAKRILVVEDNDLNRKLFGDVLKSQGFEVEPVADGREALDKARS
                     FAPDLIIMDIQLPHVSGLELIAAARGDAVLAGVPVLAVTAYAGKGDEDAIRAAGAAGY
                     LAKPVSIGPFMATVKGLLEG"
     gene            17959..18264
                     /locus_tag="VFF98_03580"
     CDS             17959..18264
                     /locus_tag="VFF98_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HZU62742.1"
                     /translation="MRLTILRDPAPVPPDPEALALAALGWVLADDAHAGRFLALTGLA
                     PDDLRPRLADRAFLAEVLDFLLRHEPDVIACAAALGVPPQALGAAFHALSPPCEDYP"
     gene            18264..18881
                     /locus_tag="VFF98_03585"
     CDS             18264..18881
                     /locus_tag="VFF98_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU62743.1"
                     /translation="MARPLIVSDCDEVLLHMVGPFRDWLGEEHGVAFSLQGNDFAHAL
                     RHADSGELLDRAETWRLLDLFFDEAMHRQYPFAGVVEAIATLSAHADIVVLTNLTDRF
                     QAPRARQLAEHGLPLKVYTNQGPKGPALKAILEEYRPARAIFIDDIAQHHGSAAEQTP
                     DVIRLHLCGEPMLAPHIPCAHAAGHAHARIDDWATALPWLLGQLA"
     gene            18909..19376
                     /locus_tag="VFF98_03590"
     CDS             18909..19376
                     /locus_tag="VFF98_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU62744.1"
                     /translation="MTDAEIDARLAALGLTLPVAAAPVAAYVPVVVAGGLAHVSGQLP
                     FVGGELVKGRLGEDVSPEAGQQAAQACGLMILAQLKAALGSLARVERVVKLGAFVSST
                     GDFTGQPAVANGASELMVAVFGEAGKHARSAVGVPVLPLGAAVEVDAIVALSA"
     gene            19464..20612
                     /locus_tag="VFF98_03595"
     CDS             19464..20612
                     /locus_tag="VFF98_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU62745.1"
                     /translation="MTDTLSARLAPGISALPAPAWDALTDGANPFVSHAFLSALEESG
                     SVDTADSHGHTGWRAAPLLIDGPDGHLAAALPAYLKSHSQGEYVFDHSWADAWARAGG
                     EYYPKLQIAVPFTPASGPRLLTRQPALAPALIGAAEALCAQAGLSSAHVTFLEAGDAA
                     LFEAAGWLIRHDIQFHWENRGYASFEDFLGALSSDKRKNLRKERRAAQEGVTIRQLTG
                     AAIRPEHWDAFWAFYQDTGSRKWGQPYLTRAAFTLLGERMADRILLVMAFEADRPIAG
                     ALNFIGGNTLFGRYWGALVDKPFLHFELCYYQAIDAAIALGLQRVEAGAQGGHKLARG
                     YAPVRTTSAHYIAHAGFRAAVADFLARERAGVAADQLQLGAHAPFRKA"
     gene            complement(20635..22701)
                     /locus_tag="VFF98_03600"
     CDS             complement(20635..22701)
                     /locus_tag="VFF98_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU62746.1"
                     /translation="MTAYCPIPAGLRATADLPGQAAVAHLSMDGEGLITFANEVFLSL
                     TGYSAYDIMGCHHSAIIDLRSLRHICMDLGYDVGQVFHGQPGPIPLIGRNREIAWIYG
                     AITPQTPGETLGGALGEAAGFTLIGHDFGETGNRLVDQTGIIRALNQHFLVAEFDIDG
                     TLRAANASFLQAFNYTLGEILGTDHSRLVDPTFAATGEYAAFWNALHYGQSHPIRGLR
                     YGKNQREVWLDAAYYPVPDARGIPCKVVLMGRDLTLKYRIEQEAAENARRAENIDAVT
                     GLENRTAFRRSIQKYFNPINGSKAFPGLLVVFDINAFAACNTTYGHEAGDAILWIVGR
                     RIRDALRRQDMTARIDRDLFAAFIETAPQEEEGQLPLVERILAAIATPIELGREQIVI
                     TASAGVVCGFGWQLARAAGTEDILACAQAALAATKQKGKGGYRLFDAELAQAARRRQM
                     IERDLEAAMLSGEITLHYQPVVSLGSDGLVGYEALARWAHPTLGQIPPPEFIGVAEQS
                     GAIHALGRALLEQAARFIAGRDRALWVAVNVSPLQLQHSDFSSQLRDMARRCGVPPHR
                     IDIEITESASLDTSFNVQENIRALRDHGFSISLDDFGTGFSSLSQLLQLRADRIKIDQ
                     SFTRDCQDSQEKIGIIRSILGLSSAMGMKVVAEGIETPEISALLRELGCDYGQGYLFG
                     RPSPPE"
     gene            complement(22698..24455)
                     /locus_tag="VFF98_03605"
     CDS             complement(22698..24455)
                     /locus_tag="VFF98_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HZU62747.1"
                     /translation="MQHETFPATAQPATEAAGPAAEARIGLSAISRTLDAHFMPAGQT
                     IARLVETTGAVLGGLEGIGRALGDGGNEGDAADHLQAAAQGLHTEPERRARRGGQIAH
                     MRAITERLSLAAAEILRILTVLRFYTVNLKIASAGADEFVEFAEDMNLKLAEGSGHVE
                     AFRRQILAMRAHLNDMCALDEVLAGECARVVPAVPDRLIGHVAQLRCWQQALEALSRG
                     AQASITRLQAGVGRALEAIQIGDITRQRLEHVADACERLDSALADPQLPDAAGTRAHM
                     LRLLAAQLEDLTTDFAAQSGVLLAALDGMAPDCDALLGHGEQQSGLEESGLFLRDLDN
                     CIGAAHGMTSQLQRADAKAGQLSDVVLQVAGALRERVQGIENLRFDVDYMAINVNIRA
                     RRDSRIGRPVAVIADEIRICSQQLAELTQTVSGMADDLGAVSRSFEAQLVAAGGPGVA
                     DTLGAALDTIRSGARDAEAAMAGLDGNAHMIVDAVRAAARDLAICNDLARQLEALNGA
                     LREACGAAQPQPMAEGEHPLAAQLQAMAQTYTMASERAVHNRFLLPGMAALGGAAAAR
                     PGGGNGNDEDDDDLDGVFL"
     gene            complement(24469..>24833)
                     /locus_tag="VFF98_03610"
     CDS             complement(24469..>24833)
                     /locus_tag="VFF98_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HZU62748.1"
                     /translation="HAYIAPGGLHMRIKRSGSNYAIALSEDELVNRHRPSVDVLFNSV
                     AAVAAHNAVGAILTGMGDDGAQGLLEMRQAGARTIAQDEASSVVFGMPKEAIARGAAE
                     RVVPLGQVARELFQACLR"
BASE COUNT         3979 a         9074 c         7991 g         3789 t
ORIGIN      
        1 gggtcggcgc gccgaagttg taggtcagcg tcgccagcag cgagtggctc tggaagcggg
       61 cgttcacgcc gctgccatcg gtcgccacga aattgtcggc cccttcgccg cggaagaagc
      121 gatacttcag acccgcatcg aggttcttgc tcaccggcac gcgaatgcca gccagcagct
      181 gccaggcaaa gccggtggcc gagtcatcga ggccataacc ggactgcaga ccgcgctcct
      241 gcacgcgggc cacgcccacg ccgccgccca caaagccctg caggccattg tcggggccaa
      301 agtccagcag gccgttcagc atgaacgaca gtgcatcggc gctgccggaa aggccggtgc
      361 tattgctgta ggtggtgccg cccgaagtgg tcagcttgct cagcgccaca cggcgatagc
      421 tggcttcgct ttccagacgg aaaggaccga agtcataacc gacgatgcca ccgaaatcat
      481 agccagcctt gaaagcggca gtgcccgaat aggaaccggt gctggtgaca gtctggtcct
      541 cggcaataac gccgccggcg tcaccttcga cataccagga cttgtcgcgc gcgagggctg
      601 gcgtagcgag ggccgaagag gccagcgcca accctatgat gattttgcgc atgtgttttc
      661 cccttttctc aagacagagg ctaccaagtg gcgccacgtc tatatgctta gagtggggtg
      721 cgcaagcatg catgattgta aactgttgca acaatgtcac gcttgttgtg ggatataaca
      781 ccgtttcact ttggtgcgtg tccacctcag ccggaaggca ggatggcagc cgcctgcagg
      841 gccgagatca atgccgcgat ggctgtgcgg gcctgcgaat caatcaccgt gccgccactt
      901 ggcgcggcca ccagggcggg gctttgccag ccattcagat aatgggtgaa ctggccgttg
      961 gccttgttca gcaggttcat cccgttgcag ggcgcaacga agatccacgc gccggcctga
     1021 tggcaggcca gcttgccatc ctccccggcc cagtcgccac tggcgcccgt gcccaccagc
     1081 caggcggtgc catcggccgg ggcggaaggc ggcgtggtgg cgctgccctc gatggcgcaa
     1141 tggatcagcc catccaccag cgccagcgct tcgttcacat agacttcctt ctgggattgc
     1201 gcggcaaaca gcagcggcag gccgaaacgc gggctggtgc tgtcgaagct gatggtatcg
     1261 ctcatgggga aagcctccgg aaaatcagga aagggtggca agaaacagcg cggtggaaag
     1321 gccgctggtg ccctgctggc gcacatagaa cgggccattg gccaattgcc cgcgcaaggt
     1381 ggccagcgtg gatgcgccaa ggttcagctg ggcggccggt gtgctccaca tggccagcgg
     1441 cgctgccagc ggtccatagc agacgatata ggcctcgctt tcctcctgca gcgccacatc
     1501 cacaccgtcc agccacagcc aggccccgcg cgcgcggcgc gtccagccca gcgtcaggct
     1561 gccatcggca ttggccacgg catttggatg caccggcgcc agcggcttca gcccgatgcc
     1621 gcggttgacg atatcggcct ctaccgcgct ggcatcgccc agccccagcg cggcgatccg
     1681 ggcatcggcg ccgatctggc tggaatccag cgtcaccggc aggctgtcga gcagcacgaa
     1741 gggctcgccc gccgcatggc cggcgatcgc cgcttcggtg cccccgcgcc cccgcagcag
     1801 gcccgacagc tgccacaccc cgccccccag cgccgccgcg ccggcaaact ggatgatctc
     1861 tgcccccacc agcgcccggt tggcgcccgt caccagctgg cgggcggtgg cgccggccag
     1921 ggccatgtcg ctggccagca actgcacatc aagggtcgag gccctgtcga agaacagcgg
     1981 cgaggccgcc ggcagcgccc cttgcgtagt gccgatcgtg ctgcgcagcc gcccgctggc
     2041 gctgatctgg gtcagcgcgc cggcctcgtc atccaccagt atcgccgccc ccgcccagcc
     2101 gctgctggcc gaggagaccg ccgcctggat cagcgtctgg gcattgctgc ccgtgccatc
     2161 ccagggcagc tcataagcaa agagcgcgct ggcccccacc gccacatcgg ctgccaggct
     2221 ggccgcgccg gcatagaccg cagcgctggt gcccgccgtt ggcgccagcc gttccagcgc
     2281 cagttcgatg ccggtatcgc gccattccca ttcggtcacg cgccactggc ccgccttgtc
     2341 cggcaggctc accaccgcgc cgggcgccac ggccgggtcc agctgggcgc agcgccaggc
     2401 gattgtctgg cgcgcccagt tggtattgcg ggcggtctgg gcgatcaggc caaaggcgtt
     2461 cagcgcggtg atcgcggccg gcaggtcgat ggatttgggc tggccgggca ggggctggcc
     2521 gggcgcccgc tgtgatccgg gctgataatc cagcgcaatg tcataatagc gcaggatccg
     2581 cggcgggttt tccgtcaccg gcgccagctt gcggctgaaa ccgtgcttgc cgccgaaatt
     2641 gccctggttg cctgccgcca ccgccggctc gcccagcgcg atgggcgcgc tctgcagccg
     2701 ctggcgtgat agcgtcagcc cgctgccacc ggcatcgcaa tccatcggaa acaccggctg
     2761 gaactgcgac agcgtggcat tgaacgcgcc atcgctggaa tagccggcaa tgccggtcag
     2821 cggcacatcg gcatccacat cctccagcca gccctcgaac aggctggcca gcgtcagcgt
     2881 cccgctgtcg gcaaacacct cgaaggtcag cgaggggatg cggttgccat aatcggccag
     2941 ctgcagatcc tcgaacacca cataggcaat gccgcgaaag gcggggcaat tggccgcccc
     3001 ctcattggcg gcgatcagcg gatcggcggc ctggtcctgg ctgccatcat agatgcgcaa
     3061 ggtcacgccc accttgaggt cgccggcggc cccgcgcagc aattgcccat ccgcccagat
     3121 ccgcccgatc gacaggatct gccggctcgc caccgccacc gcgaaagagg aggtgtagct
     3181 gtaggtcgtc accgaaacgc tgctcttgcc gccgcccgac gtactggcat gctccaccag
     3241 atcggtcgcc cagatgatcg agccggcgct gcgcatctgc ccgaaatggc gggcaatcgc
     3301 cgtgccatag gtcgatgtgg acaccgacag gtccttcagc cgcggcccct gcacatcggt
     3361 gccaaacagc gaattgtcga tttcgcgccc ggccattgcg cccagcacgc cgcccagcgg
     3421 cccgccgacc agcgtgccca gggccgtgaa caccaaggtt gccatgaatg cctcgtcggt
     3481 tggaaaggtc aggggggttt gaaaagggaa agaagaaggt caggcatcag gcgcaaggcg
     3541 ccagtgccgc agccgccgcc cctccggcag cgccggcgag atcaccacgc gccgcagccc
     3601 ggcatgggca tgcacgcagg cccccccctc caccgccacc gccagatgaa actggcacgg
     3661 ccccaccgcc agcagcagca catcgcccgg cagcaccgcg cccgtcgccg gcctcagccc
     3721 gcaatgcgcg gcctgtgcca gcagcggccc gatctcgcgc atccgcagcc gataggccac
     3781 cggcgccccg ccgcccgcgc caatggccag cgccaccagc cccacgcagt ccagcccgct
     3841 ggcaggatcg cgcccatgca gccgaaaggg cgcccccacc agcgcccgcg cccgcgccgc
     3901 cacttgcgcg ccggccgcct ctgccggcat caggccagtc catagcgcag gatcaggtca
     3961 ttgcccggca ggaagggctc gccctgaaaa ttcagcgtat tgccaaagcg gccggcgcaa
     4021 gtggccagcg tgcgatcgca tccttccagc agcaacaccc gcaccacgcc cgccagcggc
     4081 cggtccagcg gcgtgtcgag caccgccgtg ctgcccgcgg catcgacgat ccccatcgcc
     4141 atgccggcat agggcccatc cagccagcgc aaggcgccgc tcaccagatg ggctgcggcc
     4201 acgtcgctgc tcagcgtcac cgcattggcc gtgctgtcat aagcggtcac actcgcctca
     4261 tgggtatagc tcacggccga cagcgcgcag cccgtcccgc agaaatcggc gcggcaggtg
     4321 gggctggtgc gcggcacgat atcgcgcgcc agctctgcct tgcgagacag cagctcgccc
     4381 acaaacgtgc catcctcggc gctcacctgc cccaggctgc cgctgtagat cggctggtgc
     4441 tccagcgtct cccagtccac cagccccacg atcacccgcg cattgtcgaa gcggccggaa
     4501 accaggtcct cgccattcag cgcggcatgg gtgatggcgc cggtcacttc ggccgaatcg
     4561 ctgtcgaaag cggcggatcg gcggatcgcg gcggggatca tccccgggca ggcctgatgc
     4621 agcacgccat cgaaccacag gtcggcatca tgggtggtaa agcccagcgc cacgccatcg
     4681 gtgcgggcaa tgcgccaata ggtggccacc gcctccagcc cttgcgaaaa ccacaccctg
     4741 ctcataccgc ctcgcggatt tcgacgacgg gcacgctggg cgcctcgctc gcggcaaagg
     4801 tcacgccggt cacctccagc ttgtcctgct cgaagcgcac cggcacatcg aaacagaagc
     4861 ccgccgccac ggccacgccc gccgctggcg ctgtggcaaa agtgatcatg ccgccatcgc
     4921 ccggcgtcca gccgctggcc tgttccaccc cgcccaggct cacccgcacg gtgccggcgc
     4981 gcggccgggt gatccggcgg atctgctggc agcccgcctc gccataggcc ttcaccagcg
     5041 caaagctggc cgtggcgcca tcgcccgtgc ccagcgcctg atcggcaggc cccggcaccc
     5101 ctgtcatgcc gttcgagctg aaatcgctgg gatcggccag ccggaagccg cgcgcggccc
     5161 cacgcctggc gcggaagaag gcgatcagcg tgcccagatc cccttccgat cgcacccccg
     5221 gccccacatc gaagctgagc cgcgcatcgc gccacacgct ggcccggtgc tcaaagcccg
     5281 aggcggtggt ggtgatggtg gtggaaaagg ccgggctcac atgggcatca aggcccagcg
     5341 gcagcggata gaccacatcg tcgaaggcct gcatgtcttc ctcatcctcg gttgatgcca
     5401 tatgcacaaa gccgtcgcgc accacctgcg gcagagccca cacgaacacc tcgtgcacgc
     5461 cgcgcgcaaa ggcctcggcc atccccgcat ccacccggcg ccacaggtcg gcctgggtgg
     5521 catcaggcac atagccggcc agataatcct gctgcgccac gggatagccc agccggctgt
     5581 ccacctgctg ataggcctgc cggcgcaagc catcggcgcc ggcggtcagc cagtcgtaat
     5641 cctccacctg cagccggtcg aaggcgggcg ccgcccagcc cagcggcagg ttggcgcgct
     5701 gcgcctcggg cgttgccggg ttcagcaccg tgggcgtgaa caccagcagc aacacctcgg
     5761 cgcccgccgt gcccgccgct gcccgcaccg ccgccaccag ccccgccgtg gcgctggcca
     5821 gcaaggcgcc ggcggcatcc agcagggcgg tctgggcgct ggtcaggggt tgggtcaggt
     5881 cggcaatggc caccgggctg ccgcccagcg ccgccgcggc cgccgcatcg taaaggcaga
     5941 tgccatggct ggcggtgatc caccaccatg gctcgccgat ctgcacccgc accgccacgc
     6001 cggctgcctg cagcagcccg gcataggccg ccgccacctg ctgcaaccag cccatcgccg
     6061 ccgcattggc cggcgacagc agggtggagg gtggctccca tgcggtcagc gccggattgc
     6121 cggcactgtc catctgctgc cagccggccg ggcaatattg cgccagcacc tcgaacgaca
     6181 gcgaggcaat cggcgacagc cccgccgctc cgcagcgctg gaaaaactcc gcatgccagg
     6241 cccgcgccgg cgcattcagc ggatcgccgc cctggcccac cagataggcg ccgcccacat
     6301 cggccagccg cgtatggtgg ctcatgccca cataatgcag cacgctgccg cgatagccca
     6361 gctggcgcag gccgcgcacc agccgcgccg ggctctggtt gccctgatca tcaaagctgg
     6421 tggcgatcgc cccgccgccg ggcggcacga acacattgcc gatcttcagc atcgcccccg
     6481 cgcccgcaca gcgcaggccc gcaagctgcg cccagccatc aacggcggcc gccagcggcg
     6541 ttgccgcgcc gctatagcca ggcgctgcca gcgagatgaa catccggctg atcgctgccg
     6601 gccacaccgg atcggccccc gccccgctcg tcgcccagcc gcccaccagc gccgagaaag
     6661 gcaggctgat cgccgcatcg gtgggcgtgc cgctggcata gttccacagc cgcacatacc
     6721 atgtatgggc cgcgccactg gcatccagcc cctcgatcgt cagcgtcggc ccgttcacct
     6781 ggtcgagcgc gatgatcccc cccgatgtcc aggtgaagga cagcgtcgcg ccggtgtaat
     6841 cgctgttcgt cgcataggcg agcagcggat gatcgaggct gtcggcgctt tcccacacca
     6901 gcccggccag atccgcagcg gtcaggaagg tcgcatccac ccggatcgca tcggcggccg
     6961 gattggtcag ggcagccatc atcggccggg ggaagttcac ggtccagaac cgcggatcaa
     7021 aacgctggat ccagccggtt tcctggccgt ctatggcaga agcaagccag caggccatgg
     7081 cgcatctcct ctcaattgcc ggcaagggcg cggcgcatgg cgctggcgat ctgccggctc
     7141 gatcgctgca gggcctgggg gccatcgctg ccttgcggcg cgttcacatt gatggaaacg
     7201 cgggtttcgc gcgcggcccc gctgccattg gcggcgatcg atccggccga ggtcggcacg
     7261 aacacttccg gcccccgctc gcccaccata taggcctgcc cgggcgaaac cgcgccgcct
     7321 gtcgcccgcc cgggcagccc ggtcagcagg ctggagacaa ggtccacccc cagcaggccg
     7381 ccgcttgcca cagcgcccgt atcgctgccc accgaggcaa acaggttgct gctggcttgc
     7441 gcggcaatat cgttgatcac ccccagcgcc gtcgtgcgca ggttggtgaa gcccgtcacg
     7501 cccttggcca gcgcattggt cagccctgta tccagcgtgc tgccggcctg ggtaaagccg
     7561 gtcagcagcg tgccatccac gctgctgcgc atggcggcga tatcctgggc gaaattctgc
     7621 gtgctggcac gcacgtcgat ggtcaggctt tgcgacgtgc tgccggtgct cgatgtatag
     7681 cggctagagc tgctcatggt ctcgctccat caatcgattc atttccgcgc gggtcagcgg
     7741 cagggggccc tggggggcgc cttcggggcg cagtatggcg gccagctcgg ccggcgttgc
     7801 cgcccagaac tcggccggcc gccagcccag cagccgcgcc gaaagccccg ccagccgccg
     7861 cgccgaagcg ccaaagccgc tcacgccgcc ccttgcagga tctgctcgag cagggcgcgc
     7921 aagggcgcca tggccctggc cagccccagc ttcagcacag cctcgctgaa ggcctcgcgg
     7981 ctcatctcgc cggcatcggc caggcagtgc cagaacagcg cggccacctc ggccagccgc
     8041 agatgccccg cgcccgcccg ctccaccagc gcaaacagcg agcccagctc ctcttcggcg
     8101 gccaccagcg cggcaaagct gggccgcagc acgcgctcgg cgccctccag caccagcttc
     8161 gcctcgcccc gccaggggtt ggcgccgctc atgccgccac caccacgccc gagctttcca
     8221 gctgcagggt atagctgcgc tcattgttga aatcgccggc atattccagc ttctgcacaa
     8281 ggaacgcgcc ggtcatcgtg gcgccatcct cgaagcacag ctgataggca tcgatggtgc
     8341 cggccagcgc attgccccgg atccgccctt cggcggcaga gcccatgaac acgccggccg
     8401 cgctcaccga aatggaacgc gcgccgccat tgttgagcag gtcgcgccag ccattgctgt
     8461 ccttggtggt aatcaccacc gcctggccgg cgatggaaag ctgggtggtg cgcagccccg
     8521 ccaccgtctg gtaattgggc gggctggcgc catcggtaat cttcagcaga aaggcggcgc
     8581 cggattgggc ggtcatggca aatctccgtc gcaaaagggt aaaagggggt cagcccgcaa
     8641 tcgtgcggaa gcgatattcc agcagcacgg tgcgggtggt ctcggtcagc tgctcggcgc
     8701 gctggcgcat gaactgcagg ccaatgatgg tgaagccggc ctgcgcgcgc ggcatggcgg
     8761 caagggcggc ctgaatggcg gccaccagcg gggcggcatt gccgggctgg tcggcgcgca
     8821 catgcagctc gaaggcgatg cggctttcat agccggtctc ggtcttggtg ctccagtcca
     8881 cgctggcgct ggccgagatc gccagccagg gcaggctggt gaaggcgggc ccctcctcgg
     8941 taatggcatt gagctggccg gccagcgcgg catcgcccgc cagccagccg atcagcgccg
     9001 cgcgcagggg caattccatg ggccctatcc tttcgtcttc atcaggctgg cggtgaacag
     9061 cggccacacc aggctggccg tgcgccagcg cagcgggctg ccgctggctt ccagctggcg
     9121 gctctgggcc tgggcggtgg caagggtcgc ggcggcctcg gtcagcgctt cgctcagcgc
     9181 ttccagcgaa ggctggggcg tggcgctgat cataccagcc gcacccggcg gaagggccgc
     9241 cacagcgccg ccaccgaagc cggcggcaag ggcgccgcgc catcgctttc acgcgtgcgg
     9301 tgctggtggg cggccaggcg gatgatgcca tggcgcagcg gctggggcag gtggtcccag
     9361 tcgggcgcca ggccggcggt aaagctcacc accgcgcgca tcgcccgccc cggctggtcc
     9421 acgcgcacaa agccggcccc ttcggcatcc acccgctcat gccacagcgc cggatccagc
     9481 ggcgtgcggc tgccgtcggc ggccaccagc gccatctcgg cgatcgccag caccggcctg
     9541 gtatccagcg ccagccagcc ctcggcgggc agggcaaagg ggttgtgcct tggccattcc
     9601 ttgggaaaac ggcgctgctg ccaggcatcg gcgcgtggca tgtggcgcca gtcggccggc
     9661 agcggcacca tttcctcgca gccgcaggcc agcggcatca gcccggtaaa atccgcgcag
     9721 acatcgagag cggcggccag cagcgcggcg agcggcgcat cgtcctggcc ggtggtgatg
     9781 cccagccatt gcttcagctc ggcgagcgcc tctgccggca acaccgccgg gttgacgatt
     9841 acccgcatca tctacggtct ccgttcgctc tatctggaaa aaaggggccc gcgccgccag
     9901 gcaaggcaag cggggcggcg cgggcgcggg cagcggggca gggagggccc cgccgcccgc
     9961 aatgtagccg caaggctggg gccgcggcct cagctggcgg cgatggtcag cagcttgatg
    10021 gcattgctgt ccagcacctg cccgccggtc cgcttcgtcg catagaagtt gacgaagggc
    10081 ttgttgctgt agggatcgcg caggatccgc gtgccggtgc gctcggtgat gagatagccg
    10141 gcctggaaat tgccgaaagc gatcgggctg gcgccagcgg cgatgttcgg catgtcggcc
    10201 gcctcgatca ccggatagcc cagcaggcgg tcgggctggc cgtccatcat gcccggctgc
    10261 cacagatagt cgccgatggt gtccttcagc ttgcggatct gcgccagggt agaggcgttc
    10321 atcacccaca ccgcgccctg gcgatggccc gggttcagcg ccatcaccat gtcgatcaga
    10381 tgcaggtcgg gcgaggtgtc gaagccgctg gcattgccgc tggccacata ttgcagcgtg
    10441 ccgaaggggc ggctgccatc gggcgcctcg ctggtggccg cggccaggaa gccggccggc
    10501 tggttgctgc cattgccgtt gacgaaagcg gcgccctcgg cccggccgaa ttcgcgggcg
    10561 atctcgccgg ccagccagcc ttcgagatcg aacagcgcat catccagcat ctgctggctg
    10621 gccgacgggt tggcatacag ctcgccatgc ggcggcacga tctcggcaaa gctgggctct
    10681 gtcgtttcgg ggcgggcggc cgcctcgctc acccagcccg aggcggtgcc gcccaggctg
    10741 atcagcttgc gatagtccga cgagctggtc tgcaccacct gcgccacctg gcggatcggg
    10801 ctgacccgca gcagcacctc ggcaatccgc ttgtcaagct cggtcggcac cagatagccg
    10861 gcctgcgagg gcgtgccgat caccatcgat ttcagctcgc catcgcggcc cgcccgcaga
    10921 tagccatcga caaagctctt cacctcgccg ccgccgccgg cagccgcgct gccgatcacc
    10981 gggcgcaccg gcgcgcggcc cagcctgtcg atgcgcgatt tcacctcggc catctcgccg
    11041 cgcagcgcgc ccagcgcctg ctcggcggcc tcctgccggg ccaccacatc aaaggaggca
    11101 tccagcgcct gggcggtatc atcatgttcc atggggcttt tacctttcac acaaaaaagg
    11161 ccgccccatc aggcggccac acgaaagaag gaaaacggga gagggcaggg tgctcaggcg
    11221 atcatgtgca cccgcgcccc cgcctgcatc gggttggaaa ccaggctcac ctcgaacagg
    11281 tcgatgtcgg tcaggtcccg cccggccgta tcgcgggtaa agccgcgcgc ccgatagccg
    11341 aaggaaaggc ccgtcacctt gccggccttc agcgccatcg ccgccccgcc ctgggggttg
    11401 tcgatgcgag cggtcacgcg caggccccgg gcatcttcgc tggcctcgtc gatccagccg
    11461 atgcgcacat cgggccggtg ctgccaatag agcggcaagg gcccctggcc agcccgccgc
    11521 cgctcggcca gcgtgcgggc aaaggcgccg gggcggatca catcggcccc ggcatcgcgc
    11581 ttgtcgaaca gcgccgcata gccggcaaag cgcagcgcgg catcgccttg cggggccgtc
    11641 atttcaacag ctccgtcaca tcgctgcgaa aggcgatcag caccagcagc agcgccagca
    11701 ccgcgcgcat cagccattcg cccagcaccc gccacgcccc ccgcctggcc tcgcgccagg
    11761 cggccagcag ctggcgcagc tcgctcatgt cgtcgctggc gcgggcatcg cccagcccca
    11821 tgcgggccag catccgcccc gcgcccagct cgcttgcctc ctccaccagc gcgcgcagcg
    11881 tcaccagatc gctgccctgc tccacccctt gcgcgatcag ccgcgccagc atgtcctcgc
    11941 gcatcatacg gggatgtcca caatcggcgc ggcgccggcc ggctgggcgg caaagcccag
    12001 caattcgcgc ttttccgcct gggtcaggaa gctggcggca ttcacctgcg tccacagcgc
    12061 ctgccggtcg tcggccagcg ccggcacccg gtccaggtcg atcgccagct gcgcctcggg
    12121 aaaccacgtc gccagcccct cggtcagcgc ggcaaacagc ttggccgaca gcggcaacag
    12181 cgtcagccgc cacagcgccc ggttggcctc cttgtaattg gcataggtgg catcgcccgg
    12241 cagccccagc agcatcggcg gcacgccaaa ggccaaggca atatcgcgcg cggcggccgc
    12301 tttcaggctg gcaaaatcca tgtccgccgg gctcatcgac agcgattgcc acttcagccc
    12361 gccctccagc agcagcggcc gcccggcatt gaacgagccc gaaaaggccc tgttcaattc
    12421 ctcgcgcagc cggtcgaact ggtctggcga aagccctccg ccattgcccg gatcatacac
    12481 cagcgcgccc gaaggccgcg ccgcattctc cagcagcgaa cggttccagt tggccgccgc
    12541 attatgggtg gcaatcgcct ggtccgcggc ggaaaggcag cctgcgccat aatggtcgtc
    12601 ggtgggatga tagccgcgga tgtggatcac attgggcgag gcatcgccat ccaccagcgg
    12661 gatgaccatc gtcttgtcac ccacctgata cgacagcgaa catggccagc catcggcgcc
    12721 cggcatcacc gtcactcgct cgggccgcag ggcaaacagc tccaccggcc ggccctcggc
    12781 atccttcagc acctgcacat aggcattgcc atgcagcagc agctggctgg tcagcgtttc
    12841 catcagcgcc tggccggcgc tggtcgcctt caccagcgcc agcagggcag ggtcggccgg
    12901 catcagcggc gccccgccaa tgccatcggc caccagccgc accgcgcgct gggccaccgg
    12961 attgtcgaga tagccgcgca tcaccgcgca gttgtattca tagggcaccc ggctgatgcc
    13021 ggcatcggca aacagccagg gcgaggccca ggggctggca ccgccgcgcg ccagcggcac
    13081 gcgcggcccc ttgcccttga aggcactcgc cagggattgc agcaggttca tcttgtgcac
    13141 ctttcatccg ccgcggcgaa gggcggcttg gctggaaaag aaaaggaaaa cagcagggaa
    13201 gctcagaaat ccatgcgcac acggggcgcc gcgcggggcc ccagcatcag ctcgctcagc
    13261 gcccagacca gcgcatcggc gcggtcgggc gaacggcccg ggccttgata ggccccgccc
    13321 gcgatcaacc cacacagctg gtcctccagc tcgggaaaat ggccgacatg gcgcacccgc
    13381 cccgcctcat acagcgccgc caccggctct gcccgcgccg ccttgcccac ccgcgcatgc
    13441 accagccgca ccggcaggtg caactcggcc gcgcgcaaca cgctttccac catggcgccg
    13501 ccctggttgg cctcggccac cacccggtcg gcgcgccagt gggcggccgc ctctgccacc
    13561 gcgcgggccc agcgctcggg cccggcgcgg cgcaaggtgg catcggccag cagccgcgcc
    13621 agcccatccg tgcccagcgc caccaccacg atcccgcagg catcacccgt ggccgaggcc
    13681 ggcggatcca ccccgatcac cacccgcgcc ggcgggctgg cggcggcgct ttcgcggcag
    13741 cgctccagca gggcgcggct ccacaaggcg ccctcctgct cggtaatcag ctcgccatcc
    13801 agctcttgcc gcgcccattg cgagcggccg aaagcccggc gcatatcggc cagaaagcgc
    13861 ggcggcagat gggccgcatt ggcctcgctg cgcccacggg taaccgccac cccctcgcgc
    13921 gccagcaact gcaacagcag cggcaccgcg cgcggcgtgg tggtggccat cacccgattg
    13981 tcctcgccca gccgcaggcc cagcagcaga ttgtgccagg tctgttccgc ccgcatgccg
    14041 gcattatccc atttggctat ttcgtcgcac cagccatggc tgtgctgagg cccgcgcagg
    14101 ctctccggct cggcggcgga ataaagcagc gcctgcgccc cgttaggcca caccagccgc
    14161 cgcagcgaag gctcatagcg cggccggcgc cccggcgggg caatcgccag caagccgctt
    14221 tcgccctcca ccatcaccgc gcgcacttcg cccaaagagg cccccaccag cgcgatgcgc
    14281 gcctcgggcc gcgcctgggc aatggcgcgc acccattccg ccccggcccg cgtcttgcca
    14341 aaaccgcgcc cggccatcac cagccagcag cgccagtcgc ccggcggcgg caattgctcg
    14401 ctgcgcgccc acagccgcca gtgccaggcc agctcgcgct gttcctcgcg ggtcagcgcg
    14461 gcaagggcgg catggcgctc gccatcgggc aggcccagca gccaggccaa ccgctcatcc
    14521 agcgacatcg ccaccctcgc tctcgctctc gcccccattc tcgcgccccg cctgccgctc
    14581 gcgcatccgg tccagcttgg cgttgatcga ggcgaggatg gcatcggcat cctcctcgcc
    14641 ctgcaccgcc cgctcgcgcg ccaccgtctc gcggtgggcg gtcagcaggc gcagggcggt
    14701 cgcattgtcg aaggcgcggg cagagcggcg ggcgccggcc gccggcttga tctcgccgca
    14761 gcgcagccgg cgcagcaatt ccatctccag atgatcatag ccctcgcaca gggccgcgcg
    14821 ccaggcgcgg gcaaaggccg ggtcggcgcg gcgcagctca taggccttgg tggtggaaac
    14881 ccgggccttg cgcgccgccg cgctcacatt agacgtcgcc gccagctcgg ccagaaacac
    14941 ccggcgccac tggctgcgca cgcccttgcc ggcgggcaca gcgcgcacgc gggcaatggg
    15001 ctcagaacga tccaccatag gaaagcctgt ccatagcaag gggaaggaaa gcgacgcccc
    15061 gcagcacccg gtgccgactc ggtctatcgc gatgttcccg atatgtaccg attgagcgtg
    15121 acgatgtcaa cgaaaatttg ccatatcggt taattgcacc gcacccttgc cgccccgcca
    15181 caccccttct acagcaccga tggaccgcca taaccttggg gaaatcaatg ctccgccgcc
    15241 tctacgactg gaccatggcc aaggcagagg ggcccagcgc ccctgcctgg ctggcgcttt
    15301 tcgcctttgt cgaagccagc ttcttcccga tcccgccgca tccggtgctg gggctgatgt
    15361 gcctggccca gccgaagaag gcgattcgct tcgccctcat caccacgctg gcctcggtgg
    15421 cgggcggcct gctgggctat gccatcggcc atttcgcctt cgcgctggtg ggcgaaaagc
    15481 tgctgggcct gctgcatctc acgcaaagct tcccctatgc cgcctgcaag ctgcgcggcg
    15541 atctcggctt catggccatc atcgccaagg gcgccacgcc catccccttc aagctggtca
    15601 ccatcacggc gggctttgtc ggcatgccgc tgggcaagtt cctgctggcc agcttcatct
    15661 cgcgctcgat cagcttcctc atcgtcggcg tgctgttccg cctgttcggc gcgccgatca
    15721 aggcgtggat cgatcgctac ctgggcctgg ccgtggccgg cttcgtggtg ctgatcatcg
    15781 ccggctttgc cgcggcagcg ctgctcggcc atggcggcgg cggcaaggca gatccgtgca
    15841 ccggcgccac cctgcagagc ctttcggcct ccgcctgaac gcctgaacgc aaaaggggcg
    15901 gggccgctgc aaagccgccc cgcccccttg tggcccgctc aggcctgccg gatcagagga
    15961 tcccggcctt tgcgccggcc cgctggaaca tgccgatgat cgtctgcacc tgctctgccg
    16021 tgtgcagcgc gcacagcgag cagcgcagca gcgtcatgcc cgcaggcgtt gccggcggcc
    16081 gcgccaggtt cacatacagc ccctcgtgca gcagcgcctc ccacatcgcc gcgccccgct
    16141 ccagatcggg catgatcacc gagatgatcg cgctctgcgg ctcatccgtg cccagctgga
    16201 agccggcttc gcgcaggccc ttgtgcagcg tgcggctgtt ttcccacaga tgggcgcgct
    16261 tttcgccggc atgcatcagc ttgcggatcg aggtggcagc ggttgccacc acgctgggcg
    16321 gcaggctggc ggtaaacaca tagggccggc acaccagccg catgatctcg aacttggggt
    16381 ggttcgacac gcagaagccg cccaccgtgc cgaccgactt ggagaaggtg ccgatgatga
    16441 aatcgacatc gtccagcacg ccctgctctt cggccacgcc gcgcccgttg gggccgatga
    16501 agcccatcga atgggcctcg tccaccagca ccatcgcgcc gttttccttg gcgatgcgga
    16561 tcatttcctt cagcggggca atatcgccca gcatggaata gaccccctcc agcaccacca
    16621 gcttgccagc gcccgccggc acgcgcttca ggcgcttttc catcgcctcg atgtcattgt
    16681 gcttgaaggg caccacctcg gcatcgccca gcttgcagcc atcccagatc gaggcatggc
    16741 tgtcgatgtc gaggatgatg taatcgccct tgccggcgat ggtggaaatg atgcccaggt
    16801 tcgcctgata gccggtggag aacaccatgg catggtccat gccatagaac tccttcagcg
    16861 cctcttccac cgccttgtgc ccggcatagg tgccgttcag cacgcggctg ccggtggtgc
    16921 cggcgccaaa atcatccagc gcctgcttgc cggcggcaat cacatcggca tcaaaggtca
    16981 tgcccatgta gttgtaggtg cccagcagga tcgtctcgcg cccgttgcac agggcaaccg
    17041 tcgggctgat caccttttcc atcaccagcc caaaggggtc ggtcacgccg ctggccagca
    17101 ggtcgcggcg cagggtgatc atcgggtcga acttggcgaa caggtcggcg gccacggggg
    17161 cctcgcgcgt ctcggtcatg gttgtcctca gcccttcagc ttcaccaccg catcgaccag
    17221 ctggccatag gtctcgatct cggcctgctg gctcatcgag atgatgatgt cgaactcgtc
    17281 ctcgatcgcg gcgacgaagt ccatcaccgt caggctgtcg aattcaaggt cgccggcaaa
    17341 ggtggtggca tcggcaaggg gggcgccctt cttgttgaag gggccgatca gctcggcgat
    17401 cttggcttgg gtggcggcgc ggtccatcgg tgaggctccg gataaaacga tgaggacggc
    17461 ccttggccga caattgcggc gcaaaagggg cgggcgcggc ataaagtcaa gaggccgcgc
    17521 ccgaagaagg gccggttcag ccctccagca gccccttcac cgtggccatg aacggcccga
    17581 tcgacaccgg ctttgccaga tagccggccg cgcccgccgc gcggatggca tcctcatcgc
    17641 ccttgccggc ataggcggtc accgccagca ccggcacgcc cgccagcacc gcatcgccgc
    17701 gcgccgccgc gatcagctcc agccccgaga catgcggcag ctggatatcc atgatgatga
    17761 gatcgggcgc gaaactgcgc gccttgtcca gcgcctcgcg gccatcggcc accggctcca
    17821 cctcgaagcc ctggctcttc agaacatcgc caaacagttt gcggttgagg tcattgtcct
    17881 cgacaaccag aatacgcttt gccatggggc cttccatcag ccatgcggaa tgtgcccgaa
    17941 tggctttagg cttagtgcgt gaggctgaca attcttcgcg accccgcccc tgttccgccc
    18001 gatccggaag cgctggccct ggccgcgctg ggctgggtgc tggccgatga tgcccatgcc
    18061 gggcgtttcc tggcgctcac cggccttgcg cccgatgatc tgcgcccgcg cctggccgat
    18121 cgcgccttcc tggccgaagt gctcgatttc ctgctgcgcc acgagcccga cgtgatcgcc
    18181 tgcgccgcgg cgctgggcgt gccgccacaa gcgcttggcg ctgccttcca tgccctgtcc
    18241 cccccttgcg aggattaccc ctgatggccc gccccctgat cgtctccgat tgcgacgagg
    18301 tgctgctgca catggtcggc cctttccgtg actggctggg cgaggagcat ggcgtggcct
    18361 tctcgctgca gggcaatgat tttgcccatg ccctgcgcca tgccgacagc ggcgagctgc
    18421 tcgaccgcgc ggaaacctgg cgcctgctcg atctcttctt cgacgaggcg atgcaccgcc
    18481 aatatccctt cgccggcgtg gtggaagcca tcgccacgct ttcggcccat gccgatatcg
    18541 tcgtgctgac gaacctcaca gaccgcttcc aggccccgcg cgcgcgccag ctggcagagc
    18601 acggcctgcc gctcaaggtt tacaccaacc agggtcccaa gggcccggcg ctcaaggcca
    18661 ttctcgagga ataccggccc gcccgcgcca tcttcatcga cgatatcgcc cagcaccacg
    18721 gttctgccgc cgagcagacg ccggacgtga ttcgcctgca tctgtgcggc gagccgatgc
    18781 tggcgccgca tatcccctgc gcccatgccg ccggccacgc ccatgcccgg atcgatgatt
    18841 gggccacggc gctgccctgg ctgctgggcc agctggcctg atctccctct ctcaacagga
    18901 tgacgatgat gaccgacgcc gaaatcgatg cccgcctggc cgccctgggc ctcaccctgc
    18961 cggtggcggc cgcgcccgtg gccgcctatg tgccggtggt ggtggccggc gggctggccc
    19021 atgtctcggg ccagctgcct ttcgttggcg gcgaactggt gaagggccgc ctgggcgagg
    19081 atgtcagccc cgaggccggc cagcaggccg cccaggcctg cggcctgatg atcctggccc
    19141 agctgaaggc cgcgctcggc tcgctcgccc gggtggaacg cgtcgtcaag ctcggcgcct
    19201 tcgtcagctc gacgggtgat ttcactggcc agccggcagt ggccaatggc gcctcggaac
    19261 tgatggtggc ggtgtttggc gaggccggca agcatgcccg cagcgccgtg ggcgtgccgg
    19321 tgctgccgct gggcgccgcg gtggaagtgg atgccatcgt cgccctcagc gcgtgaggcg
    19381 cggctgatct ccggctggcg cgggccccct tcgcgggtgc tggcgccagc cgcccggcgc
    19441 tggcataatc ctcccgcccg ctcatgacag acacgctctc cgcccgcctc gcccccggca
    19501 tctcggccct gcccgcgccc gcatgggatg cgctgaccga tggcgccaac cctttcgtct
    19561 cccacgcctt cctcagcgcg ctcgaggaat cgggcagtgt cgacaccgcc gacagccacg
    19621 gccacaccgg ctggcgcgcc gcccccctgc tgatcgacgg gcccgatggc cacctggcag
    19681 ccgccctgcc ggcctatctc aaaagccatt cccagggcga atatgtgttc gaccatagct
    19741 gggccgatgc ctgggcccgc gcgggcggcg aatattaccc caagctgcag atcgccgtgc
    19801 ccttcacgcc ggcctctggc ccccgcctgc tcactcgcca gccggcgctg gccccggcgc
    19861 tgatcggcgc ggcagaggcc ctgtgcgcgc aggccggcct ctcctctgcc cacgtcacgt
    19921 tcctcgaggc gggcgacgcc gccctgttcg aggccgccgg ctggctgatc cgccacgaca
    19981 tccagttcca ctgggaaaac cgcggctatg cctccttcga ggatttcctc ggcgccctct
    20041 cgtccgacaa gcgcaagaac ctgcgcaagg aacgccgcgc cgcgcaggaa ggcgtcacca
    20101 tccgccagct caccggcgct gccatccgcc cggaacactg ggatgccttc tgggccttct
    20161 atcaggatac cggcagccgc aaatggggcc agccctatct cacccgcgcc gccttcaccc
    20221 tgctgggcga acgcatggcc gatcgcatcc tcctcgtcat ggccttcgag gcagaccggc
    20281 cgatcgccgg cgcgctcaat ttcatcgggg gcaacacgct gttcggccgc tactggggcg
    20341 cgctggtgga caaacccttc ctccatttcg agctgtgcta ttatcaggcg atcgatgccg
    20401 ccatcgccct gggcctgcag cgcgtggaag cgggcgcgca aggcggccac aagctggccc
    20461 gcggctatgc gccggtgcgt accacctcgg cgcattatat cgcccatgcc ggctttcgcg
    20521 cggcggtggc cgatttcctg gcgcgcgagc gggcaggggt ggcggcagac cagctgcagc
    20581 tgggcgctca cgcccccttc cgcaaggcct agaacgcctc attccgccag tgcctcattc
    20641 gggcggcgat ggccggccaa acagatagcc ctggccataa tcgcagccca gctcgcgcag
    20701 cagggcggaa atttccggcg tctcgatccc ttcggccacc accttcatcc ccatcgcgct
    20761 gctcagcccc aggatggaac ggatgatgcc gatcttctcc tgcgaatcct ggcagtcgcg
    20821 ggtaaagctc tggtcgatct tgatccggtc ggcgcgcaat tgcagcagct gcgacagcga
    20881 ggagaagccg gtgccaaaat catcgagcga gatgctgaag ccatgatcgc gcagggcgcg
    20941 gatgttttcc tgcacgttga agctggtatc cagtgaggct gattcggtga tctcgatatc
    21001 gatgcggtgc gggggcacgc cgcagcgccg ggccatatcg cgcagctggc tgctgaaatc
    21061 gctgtgctgc aactgcagcg gcgagacatt caccgccacc cacagcgccc tgtcgcgccc
    21121 ggcgatgaag cgcgcggcct gttccagcag cgcccggccc agcgcgtgga tcgcgccgga
    21181 ttgttcggca acgccaatga actcgggcgg cgggatctgg cccagcgtgg ggtgggccca
    21241 gcgcgccagc gcctcatagc ccaccagccc gtcgctgccc agcgagacga ccggctggta
    21301 atgcagggtg atctcgccag acagcatcgc cgcttccaga tcgcgctcga tcatctggcg
    21361 gcggcgggcg gcctgggcca gttcggcatc gaacaggcga tagccgccct tgcccttctg
    21421 cttggtggcg gccagcgcgg cctgcgcaca ggcgaggata tcctcggttc ccgccgcgcg
    21481 cgccagttgc cagccaaacc cgcacaccac ccctgccgaa gcggtgatga cgatctgttc
    21541 gcggcccagc tcgatcggcg tggcaatcgc cgccaaaatc cgctcgacca gcggcagctg
    21601 gccctcctcc tcctgcggcg cggtctcgat gaaggcggca aacaggtcgc gatcgatccg
    21661 cgcggtcatg tcctggcggc gcagcgcatc gcggatccgg cggccgacga tccacaggat
    21721 ggcatcgccc gcctcatggc cataggtggt gttgcaggcg gcaaaagcgt tgatatcgaa
    21781 caccaccagc agcccgggga acgccttgga gccattgatc gggttgaaat atttctggat
    21841 ggaacggcga aaggcggtgc ggttctccag cccggtcacg gcatcgatgt tttccgcccg
    21901 gcgggcattt tcggccgctt cctgctcgat gcggtatttc agcgtcagat cccggcccat
    21961 cagcaccacc ttgcagggaa tgccgcgcgc atcgggcacc ggataatagg ccgcatccag
    22021 ccacacctcg cgctggttct tgccatagcg cagcccgcgg atcggatggc tctgcccata
    22081 gtgcagcgcg ttccagaagg cggcatattc gccggtggcg gcaaaggtag gatccaccag
    22141 ccgcgaatga tcggtgccca ggatctcgcc cagcgtgtag ttgaaggcct ggaggaagct
    22201 ggcgttggcc gccctcagcg tgccgtcgat atcgaattcg gccaccagga aatgctggtt
    22261 cagcgcgcgg atgatcccgg tctggtccac cagccggttg ccggtttcgc caaaatcatg
    22321 gccgatgagc gtgaagcccg cagcttcgcc cagtgccccg cccagtgtct cgcccggcgt
    22381 ttgcggcgtg atcgcgccat agatccaggc gatctcccgg ttgcgcccga tcagcgggat
    22441 gggccccggc tggccgtgga acacctgccc cacatcatag cccagatcca tgcagatgtg
    22501 ccgcagcgag cgcagatcga tgatcgccga atgatggcag cccatgatgt cataggccga
    22561 atagccggtg agggagagaa acacctcatt ggcaaaggtg atcagcccct cgccatccat
    22621 cgacagatgg gcaacggccg cctggccggg cagatcggcg gttgcgcgca ggcccgccgg
    22681 aatggggcaa taggctgtca caggaacact ccgtccagat cgtcgtcgtc ctcgtcgttg
    22741 ccgttcccgc cgcctggccg ggccgccgcc gcgccgccaa gcgcggccat gcccggcagc
    22801 aggaagcggt tatgcacggc gcgctcgctg gccatggtat aggtttgggc catggcctgc
    22861 agctgggccg ccagcgggtg ctcgccctcg gccatgggct gcggctgggc cgcgccgcag
    22921 gcctcgcgca gcgcgccgtt cagcgcttcc agctggcggg ccagatcgtt gcagatcgcc
    22981 agatcccgcg ccgctgcccg caccgcatcg acgatcatat gcgcattgcc atcaaggccg
    23041 gccatcgccg cctcggcatc gcgcgcgccg ctgcggatgg tatcgagcgc ggcgcccagc
    23101 gtgtcggcca cgcccggccc gccggcggcc accagctgcg cctcgaagct gcggctcacc
    23161 gcgcccagat catcggccat gccgctcacg gtctgcgtca gctcggccag ctgctgcgag
    23221 cagatgcgga tctcatcggc gatcaccgcc accggccggc cgatccggct gtcgcgccgg
    23281 gcgcggatgt tgacgttgat cgccatgtaa tcgacatcga agcgcaggtt ctcgatgccc
    23341 tgcacgcgct cgcgcagcgc ccctgccacc tgcagcacca catcgctgag ctgcccggcc
    23401 ttggcatcgg cgcgttgcag ctggctggtc atgccatggg cagcgccgat gcagttgtcg
    23461 agatcgcgca ggaacaggcc gctttcctcc agcccgctct gctgttcgcc atggcccagc
    23521 agcgcgtcgc aatcgggcgc catgccatcc agcgcggcca gcagcacgcc cgattgggcg
    23581 gcaaaatcgg tggtcagatc ttccagctgg gcggccagca ggcgcagcat atgcgcgcgc
    23641 gtgccggcgg catcgggcag ctgcgggtcg gccagggcgc tgtccagccg ctcgcaggca
    23701 tcggccacat gctccagccg ctggcgggtg atgtcgccga tctggatcgc ctccagcgcg
    23761 cggcccacgc ccgcctgcaa gcgggtgatg ctggcctgcg ccccgcgcga cagcgcttcc
    23821 agcgcctgct gccagcagcg cagctgcgcc acatggccga tcagccggtc gggcacggcg
    23881 ggcaccaccc gggcgcattc gccggccagc acctcgtcca gcgcgcacat gtcgttgaga
    23941 tgggcccgca tcgccaggat ctggcgcctg aaggcctcga catggccgct gccctcggcc
    24001 agcttcaggt tcatgtcctc ggcaaattcg acgaattcat cagcgccggc agaggcgatc
    24061 ttcaggttca ccgtgtagaa gcgcagaacc gtgagaatgc gcaggatctc ggccgcggcc
    24121 agcgacaggc gctcggtgat ggcgcgcatg tgggcgatct ggcccccgcg ccgggcccgc
    24181 cgctcgggct ctgtgtgcag gccctgggcg gcggcctgca gatggtctgc cgcatcgccc
    24241 tcgttgcccc cgtcgcccag cgcccggccg atgccctcca gcccgcccag cactgcgcct
    24301 gtggtttcca ccagccgggc aatggtctgc ccggccggca tgaaatgcgc atccagcgtg
    24361 cggctgatgg ccgacaggcc gatgcgcgcc tcggctgcgg ggccggccgc ctcggtggca
    24421 ggctgggcgg tggcggggaa ggtttcatgt tgcatggttt tgccgccctc agcgcaaaca
    24481 ggcctggaac aattcgcgcg ccacctggcc tagcggcacc acccgttccg cagcgccccg
    24541 cgcgatggct tccttgggca tgccgaacac caccgagctg gcctcgtcct gggcgatggt
    24601 gcgcgcgccg gcctggcgca tttccagcag gccctgcgcg ccgtcgtcgc ccatgccggt
    24661 gaggatcgcg ccaaccgcat tgtgcgcggc gaccgcggcg accgagttga aaagcacgtc
    24721 caccgagggg cggtggcgat ttaccaactc gtcttcggag agagcgatcg catagtttga
    24781 gcccgagcgc ttgatccgca tgtgcaagcc gcccggggcg atgtaggcat gtc
//
LOCUS       DASRYE010000065         5370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_37464,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000065 DASRYE010000000
VERSION     DASRYE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37464"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFF98_03615"
     CDS             <1..197
                     /locus_tag="VFF98_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZU62749.1"
                     /translation="LSADYLSGRARRALGLQLARLGVMAAALFGLAKWGALPLLAGAL
                     GILLARAVVLRRAGAEGVGG"
     gene            194..892
                     /locus_tag="VFF98_03620"
     CDS             194..892
                     /locus_tag="VFF98_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZU62750.1"
                     /translation="MKSSPLATEALFHIGPVPITQPVLATWCIMAVMVAGACWLTRHL
                     RLRPARGQAAMELVTGALDAQIGDVMQADPAPYRPLIGMVFLFVLVANWSGSLPGVEP
                     PTANIETDAALALIVFVATIWHGIRANGVLGYLRGFAEPSWIMLPLNLVEQITRSFSM
                     IIRLFGNVMSGVYVIGVMLSLAGLLVPIPLMALDLLTGAIQAYIFAILACVFIGAAVS
                     EGARPAATTAPPTE"
     gene            897..1139
                     /locus_tag="VFF98_03625"
     CDS             897..1139
                     /locus_tag="VFF98_03625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZU62751.1"
                     /translation="MTIEEISILAAALAVSFGAIGPALGEGRAVAAAMEAIARQPDAA
                     GTISRTLFVGLAMIETMAIYCLVVALLVLFANPFIK"
     gene            1158..1898
                     /locus_tag="VFF98_03630"
     CDS             1158..1898
                     /locus_tag="VFF98_03630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZU62752.1"
                     /translation="MHVDWWTMGLQTVNLLVLMWLLKRFLFAPLTAMVAERQAAANRL
                     LDEAAAARTDAEAARAQAEAARKAADAAREAALAAARAEVAQQREALLEQAQAQAQAL
                     RTEAEAAIARARTAEQAQLAEQAHRLAVEIAARLLAGPAGQLPVAAFLADLEQALAGL
                     PPASRASLARPEAPLQLASAQPLAEAEKAAVAAALARVLGAAVPLAYASDPALIAGLR
                     LTGPLAVAEASLRADLQRVAERLNRHDG"
     gene            1888..3396
                     /locus_tag="VFF98_03635"
     CDS             1888..3396
                     /locus_tag="VFF98_03635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZU62753.1"
                     /translation="MTADPQTGWLDEARARLASTELGGEASSFGRVVHAADGILRIDG
                     LPDARLNELLRFEGGETGLALTLDADSIGAVLLDAGEGVEAGMRAQGTGEVVRVPVGE
                     GLLGRIVDPLGRPLDGGAPITAADHWPIERPAPAIIERDLVSQPVETGVLVVDALFAL
                     GRGQRELIIGDRATGKTAIALDAIINQRDSDMICVYVAVGQRTGAIERAIAAVKAHGA
                     PERCIFVVAGAATSAGLQWIAPFAGFTMAEYFRDRGQHALIVIDDLTKHAATHRELAL
                     LTREPPGREAYPGDIFYLHARLLERAARLSAELGGGSLTALPIAETDAGNLSAYIPTN
                     LISITDGQIVLDQRLFAANQRPAVDVGLSVSRVGGKAQRPALHQVSGRVRLDYAQFLE
                     LEMFTRFGGVPDDRFRATLTRGERIRALLVQPRLRPLRGAEQVALLAALDAGVLDGLA
                     PAAVAALQERIGPALDAAAGDLVAAMQAGHKLEPPALAQLADIVRQLASTPV"
     gene            3407..4261
                     /locus_tag="VFF98_03640"
     CDS             3407..4261
                     /locus_tag="VFF98_03640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HZU62754.1"
                     /translation="MTDRLGDVEARITSVRQLSTVIGAMRGIAAVRSHEANARLAGMR
                     AYAATIGGAIAQALAMVPGGAVPAAASGRRLVIALCAEQGFVGPYNATVLEAARGVLA
                     EGGDLLIVGSRGTGAARERGLAVAETLAMAAHADQLSALANRLADRLFARLQGGAIGE
                     VLLVGARPAAGGGAARVAPVRLLPFDYARFAPASGQRPPLVQLPAAQLLARLADEYVF
                     AQLCEALTLAYAAENEARMRAMIAARENVAHRLEDLGAEARRLRQEAITGEVIELAAG
                     SLGRAEGA"
     gene            4258..4629
                     /locus_tag="VFF98_03645"
     CDS             4258..4629
                     /locus_tag="VFF98_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HZU62755.1"
                     /translation="MKIFFDGGCRPNPGPIETAVVAGGRCWLRTDHGAGDNSDGEWCA
                     LIEALRVARELGVAEPVLLGDSAMVVGQASGQRRVAARFAGHFAAFQALAAGFARVRI
                     RHVGRKQNLAGIALERLHGRI"
     gene            4676..5230
                     /locus_tag="VFF98_03650"
     CDS             4676..5230
                     /locus_tag="VFF98_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZU62756.1"
                     /translation="MARLLLLNKPYGVLSQFTDAGTDSQRPTLSGLVDVPGVYPAGRL
                     DRDSEGLLLLTDDGRLQARIADPRFKLPKTYLVQVEGDPQEAQLAQLRGGLRLKDGPT
                     LPAEAERIASPDLWPRDPPVRFRKSVPDSWIRLTIREGRNRQVRRMTAAVGLPTLRLV
                     RWQIGSWTLDDLPPGSWREVPVPR"
BASE COUNT          708 a         1810 c         2020 g          832 t
ORIGIN      
        1 ggttgagcgc ggattatctc tctggccggg cccgccgggc gctggggctg caactggcgc
       61 ggctgggggt gatggcggcg gcgctgtttg gcctggccaa atggggggcg ctgccgctgc
      121 tggcgggcgc tctcggcatt ctgctggcgc gcgcggtggt gctgcgccgg gcgggggcag
      181 agggggtggg cggatgaaaa gctcgccgct ggccaccgag gccctgttcc acatcggacc
      241 ggtgccgatc acccagccgg tgctggccac ctggtgcatc atggcggtga tggtggcggg
      301 cgcctgctgg ctcacgcggc acctgcgcct gcgcccggcc cgcgggcaag cggcgatgga
      361 gctggtgaca ggcgcgctcg atgcccagat cggcgatgtc atgcaggcag atccggcgcc
      421 ctatcgcccg cttatcggca tggtgttcct gttcgtgctg gtggccaact ggtctggctc
      481 gctgccgggg gtggagccgc ccaccgccaa tatcgaaacc gacgccgcgc tggcgctgat
      541 cgtctttgtc gccaccatct ggcatggcat tcgcgccaat ggggtgctgg gctatctgcg
      601 cggctttgcc gagcccagct ggatcatgct gccgctcaat ctggtggagc agatcacccg
      661 cagcttcagc atgatcatcc gcctgttcgg caatgtcatg agcggggttt atgtgatcgg
      721 cgtgatgctg tcgctggccg ggctgctggt gccgatcccg ctgatggcgc tggacctgct
      781 gacgggggcc atccaggcct atattttcgc cattctcgcc tgcgttttca tcggcgcggc
      841 ggtgagcgag ggcgcaaggc ccgccgccac caccgccccg cccacggagt gaacccatga
      901 cgatcgagga aatcagcata ctggcggcgg cccttgcggt ctctttcggc gccattggcc
      961 cggcgctggg cgaggggcgg gcggtggccg ccgccatgga agcgatcgcc cgccagcccg
     1021 atgccgccgg caccatctcg cgcacgctct tcgtggggct ggcgatgatc gagacgatgg
     1081 cgatctattg cctggtggtg gcgctgctgg tgctcttcgc caaccccttc atcaagtaac
     1141 gacagggcgc gccggtcatg catgtagact ggtggacgat ggggctgcag acggtcaatc
     1201 tgctggtgct catgtggctg ctgaagcgct ttctctttgc gccgctgacg gccatggtgg
     1261 ccgagcggca ggcggccgcc aaccgcctgc tggatgaggc cgccgccgcc cgcaccgatg
     1321 ccgaagcggc cagggcgcaa gcagaagccg cccgcaaggc tgccgatgcc gcgcgcgagg
     1381 ccgctcttgc cgccgcccgc gccgaggtgg cgcagcagcg cgaagccctg ctcgaacagg
     1441 cccaggccca ggcccaggcc ctgcgcacag aggccgaggc cgccatcgcc cgcgcccgca
     1501 cggccgagca ggcgcagctg gccgaacagg cgcatcggct ggcggtggag attgccgcaa
     1561 ggctgctggc agggccggcg ggccaattgc cggtggccgc tttcctggca gatctggaac
     1621 aggcgctggc gggcctgccg ccggcctcgc gcgccagcct tgcccggccg gaggcgccgt
     1681 tgcagctggc cagcgcccag ccgctggccg aggcggaaaa agcggcggtg gcagccgcgc
     1741 ttgcccgcgt gctgggggcg gcggtgccgc ttgcttatgc cagcgatccg gcgctgatcg
     1801 ccggccttcg cctcaccggc ccgctggcgg tggcagaagc cagcctcagg gccgatctcc
     1861 aacgtgtcgc agaaaggctg aaccggcatg acggctgacc cccaaaccgg ctggctcgac
     1921 gaagcccgcg cccggctggc ttccaccgaa ctgggcggcg aggcgagcag ctttggccgg
     1981 gtggtgcatg ccgccgatgg catcctgcgc atcgatggcc tgcccgatgc ccgcctcaac
     2041 gaattgctgc gcttcgaggg cggcgagacc ggccttgccc tgacgctgga tgccgacagc
     2101 atcggcgcgg tgctgctcga tgcgggcgag ggggtggagg ccggcatgcg cgcgcaaggc
     2161 accggcgagg tggtgcgcgt gccggtgggc gaggggctgc tgggccgcat cgtcgatccg
     2221 ctgggccggc cgctggatgg cggcgcgccg atcaccgctg ccgatcactg gccgatcgag
     2281 cgccccgccc cggcgatcat cgagcgcgat ctggtgtcgc agccggtgga gacgggcgtg
     2341 ctggtggttg atgcgctgtt tgccctgggg cgcggccagc gcgagctgat tatcggcgat
     2401 cgcgccaccg gcaagacggc catcgcgctc gatgccatca tcaaccagcg cgacagcgac
     2461 atgatctgcg tttacgtggc ggtgggccag cgcaccggcg ccatcgagcg ggcgattgcc
     2521 gcggtgaagg cccatggcgc gccagagcgc tgcatcttcg tggtggcggg cgcggccacc
     2581 agcgcgggcc tgcaatggat cgcccccttc gccggcttca ccatggccga atatttccgc
     2641 gaccggggcc aacatgcgct gatcgtcatc gacgatctga cgaaacacgc cgccacccac
     2701 cgcgaactgg ccctgctgac gcgtgagccg cccggccgcg aggcctatcc gggcgatatc
     2761 ttctacctcc acgcccgcct gctggaacgc gccgcgcgcc tctcggccga gctgggcggc
     2821 ggttcgctga ccgcgctgcc gatcgccgag accgatgccg gcaatctctc ggcctatatc
     2881 cccaccaacc tcatctcgat caccgacggc cagatcgtgc tcgatcagcg cctgtttgcc
     2941 gccaaccagc ggccggcggt ggatgtcggg ctcagcgtca gccgggtggg gggcaaggcg
     3001 cagcggccgg cgctgcatca ggtgtcgggc cgggtgcggc tggattatgc gcagtttctc
     3061 gagctggaga tgttcacccg cttcggcggc gtgcccgacg accgcttccg cgccacgctg
     3121 accaggggcg agcggatccg cgccctgctg gtgcagccgc gcctcaggcc tctgcgcggg
     3181 gcagagcagg tggcgctgct ggccgcgctg gatgccggcg tgctggatgg gctggcgccg
     3241 gccgcggtgg ccgcgctgca ggaacgcatc ggcccggcgc tggatgcggc ggccggcgat
     3301 ctggtggcgg caatgcaggc cggccacaag ctggagccac cggcgctggc gcagctggcc
     3361 gatatcgtgc gccagctggc cagcacgcca gtatgagcac gcggccatga ccgaccgcct
     3421 gggcgatgtg gaagcgcgca tcaccagcgt gcgccagctc tccaccgtga tcggggcgat
     3481 gcgcggcatt gccgccgtgc gcagccacga ggccaacgcg cgcctcgccg gcatgcgcgc
     3541 ctatgccgcc accattggcg gggccattgc ccaggcgctg gcgatggtgc cggggggcgc
     3601 cgtgcccgcc gctgccagtg gccgccggct ggtgatcgcc ctgtgcgccg agcagggctt
     3661 tgtcggcccc tataacgcta ccgtgctgga agcggcgcgg ggtgtgctgg cagaaggtgg
     3721 cgatcttctc atcgtgggca gcaggggcac gggcgccgcg cgtgaacgcg ggctggcggt
     3781 ggccgaaacg ctggccatgg ccgcccatgc cgatcagctt tcagcccttg ccaaccggct
     3841 ggccgacagg ctgtttgccc ggctgcaggg cggcgccatc ggcgaggtgc tgctggtggg
     3901 cgcgcgcccg gccgccgggg gcggggcggc ccgtgtggcg ccggtgcgcc tgctgccctt
     3961 cgattatgcc cgctttgccc cggcgagcgg gcagcgcccg ccgctggtgc aattgccggc
     4021 ggcgcagttg ctggcgcggc tggcggatga atatgtcttt gcccagctgt gcgaggcgct
     4081 gacgctggcc tatgccgctg aaaacgaggc ccgcatgcgc gcgatgatcg ccgcgcgcga
     4141 gaatgtggcg caccggctgg aggatctggg tgcggaggcg cgccggctgc ggcaggaagc
     4201 gatcaccggc gaggtgatcg agctggccgc tggcagcctg ggccgcgcgg agggggcgtg
     4261 aagatcttct tcgatggcgg ctgccggccc aaccccggcc cgatcgaaac cgcggtggtg
     4321 gccggcggcc gctgctggct gcgcaccgat catggcgcgg gcgacaatag cgatggcgaa
     4381 tggtgcgcgc tgatcgaggc cttgcgcgtg gcgcgcgaac tgggcgtggc agagccggtg
     4441 ctgctgggcg attcggccat ggtggtgggc caggccagtg gccagcggcg agtggcggcg
     4501 cgctttgccg gccatttcgc cgcgtttcag gcgctggctg cggggtttgc gcgggtgcgc
     4561 atccgccatg tgggccgcaa gcagaacctg gccggcatcg cgctggagcg cctgcatggg
     4621 cgaatctgag ggggccggat ttgagctggc ccgccaaagc ggctaagggc cggccatggc
     4681 ccgcctcctt ctgctcaaca agccctatgg ggtgctctcg caattcaccg atgcgggcac
     4741 ggatagccag cggcccacgc tgtcgggcct ggtcgatgtg cccggcgtct atcccgccgg
     4801 ccggctcgac cgcgacagcg agggcctgct gctgctgacc gatgatggcc gcctgcaggc
     4861 gcgcattgcc gatccgcgct tcaagctgcc gaaaacctat ctggtgcagg tggagggcga
     4921 tccgcaggag gcgcagctgg cgcaattgcg tggcggcctg cggctgaagg atggccccac
     4981 cctgcccgcc gaggccgagc gcattgcctc gcccgatctg tggccgcgcg atccgccggt
     5041 gcgttttcgc aaaagcgtgc ccgacagctg gatccgcctg accatccgcg aagggcgcaa
     5101 ccgccaggtg cggcggatga cagcggccgt gggcctgccc accttgcggc tggtgcgctg
     5161 gcagatcggc agctggacgc tggacgacct gccccccggc agctggcggg aggtgccggt
     5221 gccgcgctga gcggccttac aatggcttgc cgcaagccgt ggcgggcagg ccaagcgccg
     5281 ggttggtgag gaaggctgca tcgttcagcg tggctagaaa ggcctgcagc cgggccagct
     5341 gcgccggcgc ataggcgcgg ccgtggcggg
//
LOCUS       DASRYE010000066        11027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_144998,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000066 DASRYE010000000
VERSION     DASRYE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144998"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFF98_03655"
     CDS             <1..659
                     /locus_tag="VFF98_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62757.1"
                     /translation="WVYRQNWQRETDTAAPAGKGERRLSVTIMATAGDRARVSLTEMG
                     AASPAIEETVNADWSCAAPGTQGHWLTSRPLALPLWPGKMWEADYTARTPADPSHTRE
                     TIRQHYHAVGWEEVRVAAGSFWAMRIEEEGEWIGSWNSSAPRAAAASDSPPDAPRACR
                     ATQPLAPLRDSLYGRYHKLYWYAPAAKRWVKLVERYDDINGDPLARSSMELESYRLSS
                     "
     gene            complement(703..2073)
                     /locus_tag="VFF98_03660"
     CDS             complement(703..2073)
                     /locus_tag="VFF98_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU62758.1"
                     /translation="MASVIARRRATGSLSQRMMLAAAAWVMLLLLGGGLALDRALTGL
                     VVRNFDDQLSYMLTAMVASAEIGPDGEVFFNRPLGDQRFLEPNSGLYWQISGHGHEDF
                     PSRSLWDRSLKVPQDHFDALPHIYDSSQFEGEPLRIVERSIILPGSKTVWWFAVAESR
                     AELNGQIERIRTILVQSFMVLGLGLLLMAALQSWYGLIPLRRVRLAIQRMRTTGANQV
                     SDPLPIEVQPLVEELNELLAHNERQAEAARTHAGNLAHALKTPLTVVMNAATARAPDL
                     AETVMREASVMRRQVDHHLARARAVGRRGAGQARAEVWDSAEGVLRAVERLYDRTRFD
                     LDGKRGAAVAVERQDLDEILGNLVENAAKYGGGSVFVTIDAGKADSPLCEVWVEDDGA
                     GIPAAERSRIFDRGARLDTGKPGTGLGLAIVRDVVEIYGGHVSLGESEDLGGLLVRLE
                     LPRVKG"
     gene            complement(2135..2812)
                     /locus_tag="VFF98_03665"
     CDS             complement(2135..2812)
                     /locus_tag="VFF98_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU62759.1"
                     /translation="MRILIVEDEPTLGRQLKSTLEQNGYAVDLSVDGEDGHFMGSTEE
                     YDAVILDLGLPEIDGLTVLGMWRKEGRKFPVLVLTARDSWSDKVAGLDAGADDYLAKP
                     FQTEELIARLRALIRRASGNTSSELTAGPVRLDTRSGRVTLDGEPVKLTAQEYKLLSY
                     LLHHKGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGADVITTIRGLGYSLDD
                     PAPAKRA"
     gene            complement(2918..3226)
                     /locus_tag="VFF98_03670"
     CDS             complement(2918..3226)
                     /locus_tag="VFF98_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZU62760.1"
                     /translation="MNRLTFFRLCSAVLALSPLASVPVHAGPGEAQGEARKERRAGAV
                     LSLREIERRVLPGMSGMQYLGPEFDPEALVYRLKFIRAGHVVFVDVDARSGEVIGQSR
                     "
     gene            3350..5140
                     /locus_tag="VFF98_03675"
     CDS             3350..5140
                     /locus_tag="VFF98_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU62761.1"
                     /translation="MAAAPILSWEGLGLIQGSGWLFRDLDLHIAPRDRLALIGRNGAG
                     KTTLLKLIGGLIDADRGKRSIQPGTRVVILEQEPDFAGCAQLIDYCLAGKDAPPRHEV
                     ESIASQLGIDLTREAASASGGERRRAAICRALASEPDVLLLDEPTNHMDLAAIDWLEG
                     WLQRFNGAFVVISHDRTFLTRLTRATLWLDRGSLRRREVGFGGYEAWEEQVYAEEARA
                     AEKLDARLKLENHWLSRGVTARRKRNQGRLEKLYELRAQRASMLSPQGAAKMQLSSDD
                     TKTKSVIVADGVTKRFGDRTIIRDFSLRIQRGDRIGLVGGNGTGKTTLLKMLTGDMDP
                     DEGTVTLAKTLNGVKIDQQRSLLSPEKRVRDILAEGGDWVDVRGNKKHVQGYLKDFLF
                     DPGLVDAKVGTLSGGERSRLLLAREFARFSNLLVLDEPTNDLDLETLDLLQEVIADYD
                     GTVLIVSHDRDFLDRTVTVTLGLDGSGKVDVVAGGYADWEARRQNRTAPAPAKKPAEK
                     AEAAPPPVMPARKAKLSYKDQRDYDLLPARIEELEGEIGKLETTLHDADLYSKNPARF
                     AQLTAALDKARADKEAAEERWLELAEMVEG"
     gene            complement(5150..5617)
                     /locus_tag="VFF98_03680"
     CDS             complement(5150..5617)
                     /locus_tag="VFF98_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HZU62762.1"
                     /translation="MQRILRERELTSEGALAHPPRAGRRSVTVNVAESPLAWLHARGH
                     LEERLFLAGERLRADWERAGLASSITMRWEPVRAKGQGDGLLPTERQLAARQRFDGAV
                     AAAGRGLADVLWRVVCAGEGLPEAEKTLGWPARSGKLVLRLALDRVAEFYRIG"
     gene            5738..6319
                     /locus_tag="VFF98_03685"
     CDS             5738..6319
                     /locus_tag="VFF98_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU62763.1"
                     /translation="MINRIRDIRRQKGLTLADVAARCVPPTTAQTIGRLETGTRNLSL
                     VWMNRIGAALEVDPELLVRSEGGDSPQLVARLGPGGAEALPNPRDALLPTALSGNDGG
                     GPVVALAVEISAGEYRAGDQIWLRQVGPDSFPRLLNRDVLAPRPGGRFAFGRLLDRDA
                     QRIAILPPGVGQRQIVIDLPPWLAVAEMLIRSL"
     gene            6323..7510
                     /locus_tag="VFF98_03690"
     CDS             6323..7510
                     /locus_tag="VFF98_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62764.1"
                     /translation="MRILSVSTLFPAPGRAGFGRFVAHQMDGFAQTPGADLVMVNPIG
                     LPIWPLSRRAPYAALASCPATSPRAGYTVHHPRFRLWPLVGGTGNPARIARAVLPLAR
                     RLHAEQPFDLVDAQFFFPDGPAAMLVAQALGLPFTIKARGSDIHYWSTRPAALAQMRA
                     AAGQAAALLAVSAALKADMVALGMPEARIHVHYTGLDHARFFPRPQASARADVLAALG
                     LALPAAAPLLLCPGALIAIKGQRLAIAALASVPRAHLLLAGSGSDEPALRALAEGPAT
                     AGRVHFLGQVAPDLMAQAMSAADVVVLPSEREGLANVWIEALASGAPLVIPPIGGAAE
                     VVTTPAAGRLAARDPAAIASAINALLAAPPMREETAAAAARFSWQANAGALAAIWQQA
                     APA"
     gene            complement(7515..8498)
                     /gene="secF"
                     /locus_tag="VFF98_03695"
     CDS             complement(7515..8498)
                     /gene="secF"
                     /locus_tag="VFF98_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZU62765.1"
                     /translation="MRLLKLIPEHTNIRFLKWQWPTTIVSTLLMIGSWVLLATQGLNL
                     GVDFVGGQMIRVTFTQSAEAPIAKLRGEVEALGYGEPIIQTFGAPNAISIRLRLPAGS
                     DKNAGLADAMSKRITATIKADHADARVDGVDSVSGKVSGELGWTALKALGLAAIAISI
                     YIWYRFEWQFGVGAMASLVHDVTLVLGLFAVTQMEFDLTIVAAMLTVIGYSLNDTIVV
                     YDRIRENLKKYRKMAVPELLDLSVNETLSRTVLTSSTMLITLVALLVLGPKVIFGFAA
                     ALILGIFVCTYSSVYMAAPMLIWLKVTAGSFVPTESALDKQERLAREASRP"
     gene            complement(8514..10121)
                     /gene="secD"
                     /locus_tag="VFF98_03700"
     CDS             complement(8514..10121)
                     /gene="secD"
                     /locus_tag="VFF98_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZU62766.1"
                     /translation="MLDFPRWKQIWFWFIALAACAAALPSIFSMSGLAWPQQLPAPRI
                     NLGLDLAGGSHLLLEANPDQVVAQRLEAKEEEVRSALKRSPHAIQFGDISNKDGSLSF
                     MVGDATQVDAAREALLPLTNGAGLTGKRDWDIKVVDTTRFVLTPTKEGIDQAVGAAMD
                     VATDVIRKRIDALGTREPTIIREGQDRIVVQVPGLKDPTALKALLGQTAKLEFKLVDT
                     AAVPADVAQGIAPPGDEIVPFAAGANGASAGAIAVKRLGGIKGDQLADAKQGYNQQTN
                     EPIVSITFNTDGGAKFAQLTSENVGKPFAIVLDGKVLSAPNIREPILGGSAQISGSFT
                     VDSANSLAIALRSGALPVDLKVVEERSVGPDLGADSIHKGIIAMLVGTVAVVALMAFS
                     YGRFGIYANGALIINVLMILGVMAAFNTTLTLPGIAGFVLTIGAAVDANVLINERIRE
                     ERRRGRRVFQAVELGYKEASRTIFDANITHIIAAVLMFAFGSGPIKGFAVVLMIGIVT
                     SVFTAVFLTRMWVAGWLRRARPADINI"
     gene            complement(10307..10645)
                     /gene="yajC"
                     /locus_tag="VFF98_03705"
     CDS             complement(10307..10645)
                     /gene="yajC"
                     /locus_tag="VFF98_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZU62767.1"
                     /translation="MTMPVLSILTASATAGDGPPAWVQFLPLVAMFAIMWFLFLRPQM
                     RQQKDHKTKLEALKKGDNVLTGGGFLAKVVKVDGEYVDLDLGGNLKVRALKSTITDVI
                     PPAGAVVAND"
BASE COUNT         1840 a         3844 c         3635 g         1708 t
ORIGIN      
        1 cctgggtcta tcgccagaac tggcagcgcg agaccgacac cgccgcgccc gccggcaagg
       61 gcgagcggcg cctgtccgtc accatcatgg ccaccgcggg cgatcgcgca cgcgtcagcc
      121 tgaccgaaat gggcgcggcc agcccggcga tcgaggaaac cgtgaacgcc gactggagct
      181 gcgcggcacc cggcacccag ggccactggc tgaccagccg cccgctggcc ctgcccctgt
      241 ggcccggcaa gatgtgggaa gccgattaca ccgcccgcac cccggccgac ccatcccaca
      301 cgcgcgagac gatccgccag cattaccacg ccgtgggctg ggaggaagtg cgcgtggccg
      361 ccggcagctt ctgggcgatg cgcatcgagg aagagggcga atggatcggc agctggaaca
      421 gcagcgcccc gcgcgcggcg gctgccagcg acagcccccc cgatgccccc cgcgcctgcc
      481 gcgccaccca gccgctggcc cccttgcgcg acagcctcta tggccgctac cacaagctgt
      541 actggtatgc gccggccgcc aaacgctggg tgaagctggt ggaacgctac gacgacatta
      601 acggcgatcc gctggcgcgc agcagcatgg aactggaaag ctaccgcctg tcgtcctgac
      661 gcgcctgtct cctgacgcgc ctgtctcctg atacgggggc cttcagccct tcacccgcgg
      721 cagttccagc cgcaccagca gcccgcccag atcctcgctc tcgcccagcg agacgtggcc
      781 gccatagatt tccaccacat cgcgcacgat ggccaggcca aggccggtgc cgggcttgcc
      841 ggtatccagc cgggcgccgc gatcgaagat gcggctgcgc tcggcggcgg gaatgccggc
      901 gccatcatcc tccacccaca cttcgcacaa ggggctgtcg gccttgccgg catcgatggt
      961 gacgaacacg ctgccgccgc catatttcgc cgcgttttcc accagattgc ccaggatctc
     1021 gtccagatcc tgccgctcca ccgccaccgc cgcgccgcgc ttgccatcaa ggtcgaagcg
     1081 cgtcctgtca tagaggcgct ccaccgcgcg cagcacgccc tcggcactgt cccacacttc
     1141 cgcgcgggcc tggccggccc cccgccggcc cacagcgcgc gcccgggcca gatggtgatc
     1201 cacctgccgg cgcatcaccg aggcctcgcg catcaccgtt tcagccagat ccggcgcgcg
     1261 ggccgtggcg gcattcatca ccaccgtcag cggcgttttc agcgcatggg ccaggttgcc
     1321 ggcatgggtg cgcgccgctt ctgcctgccg ctcgttatgg gccagcagct cgttcagctc
     1381 ctccaccagc ggctgcacct cgatcggcag cggatcgctc acctgattgg cgccagtggt
     1441 gcgcatgcgc tggatggcca ggcgcacgcg gcgcaagggg atcaggccat accagctttg
     1501 cagcgccgcc atcagcagca gccccagccc cagcaccatg aagctctgca ccagaatggt
     1561 gcggatgcgc tcgatctggc cgttcagctc ggcgcggctt tcggccacgg caaaccacca
     1621 caccgtcttg ctgccgggca ggatgatcga gcgctcgaca atgcgcagcg gctcgccctc
     1681 gaactggctg ctgtcgtaga tgtggggcag cgcgtcgaaa tggtcctgcg gcaccttcag
     1741 gctgcgatcc cacagcgagc ggctggggaa atcctcgtgc ccatggccgg aaatctgcca
     1801 gtaaaggccc gaattgggct cgagaaagcg ctgatcgccc agcgggcggt tgaagaatac
     1861 ctcgccatcc gggccgatct cggcgctggc gaccatggcg gtgagcatat aggagagctg
     1921 gtcgtcgaaa ttgcgcacga caaggccggt caacgcccgg tcgagcgcga ggccgccgcc
     1981 cagcaacagc agcatcaccc aggccgccgc cgccagcatc atgcgctgcg acagcgagcc
     2041 tgtggcccgc cgccgggcga tcacgctggc catgcgcccc ggccgctggc cctgggccgg
     2101 cgccacatcc gggccggcat cctcggccat gggctcaggc gcgcttggcg ggggcggggt
     2161 catcaaggct gtagccaagg ccccggatgg tggtgatcac atcggcgccc agcttcttgc
     2221 ggatgcgggt gacgaaaacc tcgatcgtgt tcgaatcgcg atcgaaatcc tgatcgtaaa
     2281 tatgctcgat cagctccgtg cgggagacga ccttgccctt gtggtgcagg agataggaca
     2341 gcagcttgta ttcctgcgcg gtcagcttca ccggctcgcc atccagcgtc acccggcccg
     2401 agcgcgtgtc gagccggacc ggccctgcgg tcagctcgct gctggtgttg cccgaggcgc
     2461 ggcggatcag cgcacgcagg cgggcgatca gctcctcggt ctggaacggc ttggcaaggt
     2521 aatcgtcggc cccggcatca agcccggcca ccttgtccga ccagctgtca cgcgcggtca
     2581 gcaccagcac ggggaacttg cgcccctcct tgcgccacat gcccagcacc gtcagcccgt
     2641 cgatctcggg caggcccaga tcaaggatca ccgcgtcata ttcctcggtg gagcccatga
     2701 aatggccatc ctcgccatca accgacagat cgaccgcata gccgttctgt tccagcgtgc
     2761 tcttcagctg gcggcccagc gtgggctcat cctcgacaat caggatccgc atagtttttc
     2821 ctcaattcct tgatcgctga tgaaagccgc aagggccggc gtcaagcctg ccgggggaag
     2881 gccccgccac acccggcgcg accggccagc gcgacggcta gcgggactgg ccgatcacct
     2941 cgcccgagcg ggcatccaca tcgacaaaca ccacatggcc cgcgcggatg aacttcagcc
     3001 ggtagaccag cgcctcgggg tcgaattccg gccccagata ttgcatgcca gacatccccg
     3061 gcagcacccg ccgctcgatc tcgcgcagcg acagcaccgc gcctgcccgc cgttccttgc
     3121 gggcctcgcc ctgcgcctcg ccggggccgg catgcaccgg cacggaagcc agcggcgaaa
     3181 gggcgagaac ggccgagcac agccggaaga aagtgagcct gttcataaaa atcgcagata
     3241 tgctttcaga attgaacgag gggtgaatac tccgcacagc cagcccttgc gcagcccccc
     3301 ctgcgcctgg cccttgcctt ttgccgcctg cccccgctag gggccatgca tggcagctgc
     3361 acctatcctt tcctgggaag ggctgggcct gatccaggga tcaggctggc tctttcgcga
     3421 tctcgacctc cacatcgccc cgcgtgaccg gcttgcgctg atcggccgca atggcgcggg
     3481 caaaaccacg ctgctcaagc tgatcggcgg gctgatcgat gccgacaggg gcaagcgctc
     3541 catccagccg ggcacgcgag tggtgatttt ggagcaggag cccgattttg ccggctgcgc
     3601 gcagctgatc gattattgcc tggccggcaa ggatgccccg ccccgccacg aggtggaaag
     3661 catcgccagc cagctgggca tcgacctcac gcgcgaagca gccagcgcca gcggcggcga
     3721 gcggcgccgc gccgcaatct gccgggcgct ggccagcgag cccgacgtgc tgctgctgga
     3781 cgagccgacc aaccacatgg atctggccgc gatcgactgg ctggagggct ggctgcagcg
     3841 cttcaacggc gcctttgtcg tcatcagcca cgaccgcacc ttcctcaccc gcctcacccg
     3901 tgccacgctg tggctcgatc gcggatcgct gcggcggcgc gaagtgggct ttggcggcta
     3961 tgaggcatgg gaagagcagg tctatgccga ggaggcccgc gcggccgaaa agctggatgc
     4021 caggctgaag ctggagaacc actggctgtc gcgcggggta acggcgcggc gcaagcgcaa
     4081 ccagggccgg ctggaaaagc tgtatgaact gcgcgcccag cgcgcctcca tgctcagccc
     4141 gcagggcgcg gccaagatgc agctctcctc ggacgacacc aagaccaaat cggtgatcgt
     4201 ggccgatggc gtgaccaagc gctttggcga tcgcacgatc atccgcgatt tctcgctgcg
     4261 catccagcgg ggggatcgga ttggccttgt gggcggcaat ggcaccggca aaaccaccct
     4321 gctgaagatg ctgaccggcg atatggaccc cgacgagggc acggtaacgc tggccaaaac
     4381 gctgaacggg gtgaagatcg accagcagcg cagcctgctc agccccgaaa agcgcgtgcg
     4441 cgacatcctc gctgaaggcg gcgactgggt ggatgtgcgc ggcaacaaga agcatgtgca
     4501 gggctatctg aaagacttcc tgttcgatcc gggcctcgtt gatgccaagg tcggcacgct
     4561 ttcgggcggg gaacgttcgc gcctgctgct ggcgcgcgaa tttgcccgct tctccaatct
     4621 cctcgtgctc gacgagccga ccaacgacct cgacctcgaa acgctcgacc tgctgcagga
     4681 agtgatcgcc gattacgacg gcacggtgct gatcgtcagc cacgaccgcg acttcctcga
     4741 ccgcacggtg acggtgacgc tggggctgga tggcagcggc aaggtggatg tggtggccgg
     4801 tggctatgcc gattgggaag cgcgccggca aaaccgcacc gcccctgccc ccgccaaaaa
     4861 gcccgcggaa aaagcagagg ccgcgccgcc cccggtgatg cccgcccgca aggccaagct
     4921 gagctacaag gaccagcgcg actacgacct gctgcccgcc cgcatcgagg aactggaggg
     4981 cgagatcggc aagctggaaa ccacgctcca cgatgccgat ctctacagca agaaccccgc
     5041 ccgcttcgcc cagctcaccg ccgcgctgga caaggcccgc gccgacaagg aagcggccga
     5101 ggaacgctgg ctggaactgg ccgagatggt ggaaggctaa gccccctcct cagccgatgc
     5161 ggtagaattc ggccaccctg tccagcgcca ggcgcaacac cagcttgccc gaacgcgccg
     5221 gccagcccag cgttttctcc gcctcgggca ggccctcacc ggcgcacacc acgcgccata
     5281 gcacatcggc cagccccctg ccggccgccg ccaccgcgcc atcaaagcgc tggcgggcgg
     5341 ccagctggcg ctctgtgggc agcaggccat cgccctggcc cttggcgcgc accggctccc
     5401 agcgcatggt gatggaggag gcaaggccgg cgcgttccca atcggcgcgc agccgctcgc
     5461 cggcgaggaa cagccgctct tccagatggc cgcgggcatg cagccaggcc agcggcgatt
     5521 cggccacatt caccgtcacg ctgcggcgcc cggcccgcgg cggatgcgcc agagcgccct
     5581 cgctggttaa ttcccgttcg cgcaaaatcc tctgcatggc ccgattcccc ttggttcaga
     5641 cggtatagag actcttgcca aatcccccca catgtaggac agtaggtata caccgatctg
     5701 gttagcgcac tgatcacagg cgaggaatac aggcggaatg atcaaccgta tccgtgacat
     5761 tcgcaggcag aagggcctga ccctggccga tgtggccgcg cgctgcgtgc cgcccaccac
     5821 ggcgcagacg atcgggcggc tggaaaccgg cacgcgcaat ctctcgctgg tgtggatgaa
     5881 ccggattggc gcggcgctgg aggtggaccc ggaactgctg gtgcgctccg aaggaggaga
     5941 cagcccgcag ctggtggccc gccttggccc gggcggcgcc gaagccctgc ccaacccgcg
     6001 cgatgccctg ctgcccaccg ccctttccgg caatgatggc ggcggcccgg tggtggcgct
     6061 ggcggtggaa atcagcgccg gcgaatatcg cgcgggcgat cagatctggc tgcggcaagt
     6121 gggcccggac agcttccccc gcctgctcaa ccgcgatgtg ctggcgcccc gcccgggcgg
     6181 ccgcttcgcc ttcggccgcc tgctcgaccg cgatgcccag cgcattgcca tcctgccgcc
     6241 gggcgtgggc cagcgccaga tcgtgatcga cctgccgccc tggctggcgg tggcggaaat
     6301 gctgatccgc agcctttagg cgatgcgcat cctctcggtc agcacgctgt tcccggcgcc
     6361 ggggcgcgcc ggtttcggcc gttttgtcgc ccatcagatg gatggtttcg cgcaaacgcc
     6421 cggcgctgat ctcgtcatgg tgaaccccat cggcctgccg atctggccgc tttcccgccg
     6481 cgcgccctat gccgcgctgg ccagctgccc ggccaccagc ccccgcgccg gctacaccgt
     6541 gcaccacccg cgctttcgcc tgtggccgct ggtggggggc accggcaatc cggcgcggat
     6601 cgcccgggcg gtgctgccgc tggcccgccg cctgcatgcc gagcagccct tcgatctcgt
     6661 cgacgcgcag ttcttctttc ccgatgggcc ggcggccatg ctggtggcgc aggcgctggg
     6721 cctgcccttc accatcaagg cgcgtggatc ggatatccac tactggtcca cccgccccgc
     6781 cgcgctggcg cagatgcggg cggcggcggg ccaggcggcg gctctgctgg ccgtttctgc
     6841 cgcgctgaag gccgatatgg tggcgctggg catgcccgag gcgcgcatcc atgtgcatta
     6901 caccgggctg gaccatgccc gcttcttccc ccgcccgcag gccagtgcgc gcgccgatgt
     6961 gctggccgcg ctgggcctgg cgctgccggc ggccgcgccg ctgctgctgt gcccgggcgc
     7021 gctgatcgcc atcaagggcc agcggctggc gattgccgcg ctggccagcg tgccccgggc
     7081 ccatctgctg ctggccggat cagggtcgga tgagcccgcc ctgcgcgcgc tggcggaagg
     7141 gccggcaacg gcggggcggg tgcatttcct ggggcaagtg gcgcccgatc tgatggcgca
     7201 ggcgatgtct gccgccgatg tggtggtgct gcccagcgag cgcgaggggc ttgccaatgt
     7261 gtggatcgag gcgctggcca gtggcgcgcc gctggtgatc ccgccgatcg gcggtgcggc
     7321 agaggtagtc accacgcctg cggccggccg gctggcagcg cgtgatccgg ccgccattgc
     7381 cagcgcgatc aacgccctgc tggccgcccc gccaatgcgg gaggaaaccg ccgccgccgc
     7441 cgcccgcttc agctggcagg ccaatgccgg ggcgctggcc gccatctggc agcaggcggc
     7501 cccggcctga acggtcaggg gcgcgaggcc tcgcgggcca ggcgctcctg cttgtcgagc
     7561 gcgctttcgg tgggcacgaa gctgcccgcc gtcaccttca gccagatcag catgggcgct
     7621 gccatgtaga ccgacgaata ggtgcacacg aaaatgccca ggatcagcgc ggccgcgaag
     7681 ccgaagatca ccttgggccc cagcaccagc agcgccacca gcgtgatcag catggtggac
     7741 gaggtcagca ccgtgcggct cagcgtttcg ttgaccgaca ggtcgagcag ctccggcaca
     7801 gccatcttgc ggtatttctt gaggttttcg cggatgcggt cgtacaccac gatggtatcg
     7861 ttcagcgaat agccgatcac cgtcagcatc gcggcaacga tcgtcaggtc gaactccatc
     7921 tgcgtcaccg caaaaaggcc cagcaccagc gtcacgtcgt gcaccaggct ggccatggcg
     7981 cccacgccaa actgccattc gaagcgatac cagatgtaga tcgagatggc gatcgcggcc
     8041 agacccagcg ccttcagcgc cgtccagccc agctcgcccg aaaccttgcc cgagaccgaa
     8101 tcgaccccat ccactcgcgc atcggcatga tccgccttga tggtggcggt gatccgcttc
     8161 gacatcgcat cggccagccc ggcgttcttg tcagagccgg ccggcaggcg caggcggatc
     8221 gagatggcat tgggcgcgcc aaaggtctgg atgatcggct cgccatagcc cagcgcttcc
     8281 acctcgccgc gcagcttggc gatgggtgcc tcggcgcttt gggtgaaggt gacacggatc
     8341 atctgcccgc cgacgaaatc gacgcccagg ttcagcccct gcgtggccag cagcacccag
     8401 ctgccgatca tcagcagcgt gctgacgatg gtggtgggcc actgccactt gaggaagcgg
     8461 atgttggtgt gttcgggaat gagtttcaaa aggcgcatgg ggggcccctc ggatcagatg
     8521 ttgatgtcgg cggggcgcgc gcggcgcagc catccggcca cccacatgcg ggtgaggaat
     8581 acggcggtga acaccgaggt gacgatgccg atcatcagca ccacggcaaa gcccttgatg
     8641 ggcccggagc cgaaggcgaa catcagcacg gcggcgatga tatgggtgat gttggcatca
     8701 aagatggtgc ggctggcctc cttgtagccc agctccaccg cctggaacac gcgccgcccg
     8761 cgccgccgct cctcgcggat gcgctcgttg atcagcacgt tggcatccac cgccgcgccg
     8821 atcgtcagca cgaagccggc aatgcccggc agcgtgagcg tggtgttgaa ggcggccatg
     8881 acgcccagga tcatcagcac gttgatgatc agcgcgccat tggcatagat gccgaagcgg
     8941 ccataggaaa aggccatcag cgccaccacc gcaaccgtgc ccaccagcat ggcgatgatg
     9001 cccttgtgga tcgaatccgc gccaaggtca ggcccgacgg agcgttcctc caccaccttg
     9061 agatcgaccg gcagcgcgcc ggaacgcagg gcaatggcca ggctgttggc gctgtccacc
     9121 gtgaagctgc cggagatctg ggcgctgccg cccaggatcg gctcgcggat gttgggggcc
     9181 gagagcacct tgccatccag cacgatggca aagggcttgc caacgttctc gctggtcagc
     9241 tgggcgaatt tggcgccgcc atcggtgttg aaggtgatgg agacgatcgg ctcgttggtc
     9301 tgctggttat agccctgctt ggcatcggcc agctggtcgc ccttgatgcc gcccagccgc
     9361 ttcacggcaa tcgcgccggc gctggcgcca ttggcaccgg cggcaaaggg cacgatctcg
     9421 tcacccgggg gggcgatgcc ctgcgccaca tcggcgggca cggcggcggt gtcgaccagc
     9481 ttgaattcca gcttggcggt ctggcccagc agggccttca gcgcggtcgg gtccttcagc
     9541 cccggcacct ggacgacgat gcgatcctgg ccctcgcgga tgatggtcgg ctcacgcgtg
     9601 cccagcgcgt cgatgcgctt gcggatcacg tcggtggcca catccatcgc cgcgcccacg
     9661 gcctgatcga tgccttcctt ggtgggggtg aggacgaagc gggtggtatc gaccaccttg
     9721 atgtcccagt cgcgcttgcc ggtaaggccg gcgccattgg tcagcggcag cagcgcctcg
     9781 cgggcggcat cgacctgcgt ggcatcgccc accatgaagc tgagcgagcc atccttgttc
     9841 gaaatatcgc cgaactggat tgcgtggggc gagcgcttga gggcggagcg gacctcctcc
     9901 tccttcgctt ccagcctttg cgccaccacc tgatcggggt tggcttccag cagcagatgg
     9961 ctgccgccgg ccagatccag cccgaggttg atgcgcgggg cgggcagctg ctgcggccag
    10021 gcaaggccgc tcatcgagaa gatcgaaggc agggccgccg cgcaggccgc cagcgcgatg
    10081 aaccagaacc agatctgctt ccagcgtgga aaatcaagca ttgtcgggcg gctcggctct
    10141 ctagtgcggg gtgcaggcag ggttggccgg cagccctttc gggtgaggaa ctggtgttca
    10201 aggccccgcc cggcatgcgc cagacggggc cttggcatgg cagatggcga ggcaaccggc
    10261 tttggtcaag cctcgcgcat ggtttgcccg atcagtcgtt ggcggctcag tcgttggcga
    10321 caaccgcgcc ggccggcggg atgacatcgg tgatggtgga cttgagcgcg cgcaccttca
    10381 ggttgccacc cagatccaga tcgacatatt cgccatcgac cttgaccacc ttggcgagga
    10441 agccgccgcc ggtcagcaca ttgtcgccct tcttcagcgc ttcgagcttg gtcttgtggt
    10501 ccttctgctg gcgcatctgc gggcgcagga acaggaacca catgatcgcg aacatcgcca
    10561 ccagcggcag gaactgcacc caggcgggcg gcccgtcgcc ggcagtggcg ctcgcggtca
    10621 ggatggaaag cacaggcatg gtcatcggga aaggatctct cttcaatcgg cgagggcccg
    10681 cccgcaggcc cgaaagccac aggcagacct gttgcacaag caggccgggc atgccccggg
    10741 ccggaaaaaa gcctcttgcc tgatcccccg cacaccggcg ccctgccgcc agcccgcttg
    10801 ccacgggcgc ctagcagcta ttgccggccg tggcaatgaa accgcccccc ggcgcggcgg
    10861 ggtgtgcggc cggatgcggg ccccggcgat ttttttcaaa cccccgcgcc tttttcaaaa
    10921 acaggtgctt gccatgcgcg aaaaccctgc ctataggcgc cgctccggtc gggacgtagc
    10981 gcagcctggt agcgcatcac actgggggtg tgggggtcgg aggttcg
//
LOCUS       DASRYE010000067        10693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_145007,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000067 DASRYE010000000
VERSION     DASRYE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145007"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..699
                     /gene="folE"
                     /locus_tag="VFF98_03710"
     CDS             88..699
                     /gene="folE"
                     /locus_tag="VFF98_03710"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZU62768.1"
                     /translation="MGCPEDDHQESAASAKPPVPENVQEAIRTLIRWAGDDPQREGLI
                     DTPARVARAWLEYCQGYAEDPAVHLSRQFEEVGGYNELVLLKDIPFQSHCEHHMAPII
                     GKAAIAYMPRDRVVGISKLARVLHGYASRLQIQERLTAEVAQCIWDNLKPHGVAVVIE
                     AQHGCMTGRGVRTPGVGMVTSRLIGCFLDDPRSRKEVLSLMGY"
     gene            862..1653
                     /locus_tag="VFF98_03715"
     CDS             862..1653
                     /locus_tag="VFF98_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZU62769.1"
                     /translation="MAGGNRSGRRQALAVFCAAGALGWGGTAQAQYRDTTFIAAEGAD
                     SGVCDIYNPCATFGYALYETVAGGNIIVLDSGNYGNAVIAKSVTIHGEGGQMTVIGAL
                     EVNAAATDKVVIDNLAFEGTYSGGGYAFGVKVDLAKDVLIQNCRFKDYDLSTDEEGGV
                     VIAGSSGSVRLTVNNSTFFANAVGVLVESGASGTSHAKIYNSAFVSNTQAGVRVVGAT
                     NDVTLMGNVLLASPKALDLQSSGVARSYGNNVITSGDAPSALTMY"
     gene            complement(1694..3172)
                     /locus_tag="VFF98_03720"
     CDS             complement(1694..3172)
                     /locus_tag="VFF98_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU62770.1"
                     /translation="MSLFAEYDQYDATGLAELVRERAVSATELVQTALARIDSRNPQL
                     NAVVNRLDRAALAQAASFDDRSAQHATGAFAGVPFLIKDSGIQLSGERTSNGSRFWTD
                     HRAPHDSTITGRYRAAGLVILGFTNTAENGLASETAPIVYGPTLNPWDPSRSAGGSSG
                     GAAVAVAGGLVPVAHATDGGGSIRVPAANTGLFGLKPSRGRNPFGPDIGEGWNGLSVH
                     HVITRSVRDSAALLDATHGAEPGDPYAAPAQKGRFVDALTRPLSPLRIALQTVGHDGT
                     AVDPVVKAAAQDTALLLRDLGHQVEEARPQIDLAAVKRATRIIVAANAANVLATRGQH
                     IGRAATEADVEPITWLWAAEGRRHSAQELAWAVTTIHQLSRAFGRFFERYDVLLTPTL
                     ADPPLRLGTIDMQSTDLDAYFDTLYHHTIFTSVYNCAGLPAATVPLIWSNGLPIGVQI
                     AGPLGAEARLLTVAGQLEAIRPWALKRPPLHESRLTDPAVAG"
     gene            complement(3200..4375)
                     /locus_tag="VFF98_03725"
     CDS             complement(3200..4375)
                     /locus_tag="VFF98_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62771.1"
                     /translation="MTGRTHRLPADAAQVVAFAAITATALLAAPAIAAQLASQFHLPP
                     SAIGFYFTVEQGGMCLAALPALWWLGRVPWRQVAGLAMAAFILANLASAQVSHFAWLV
                     PLRAISALSGGTLMVLSMTLAGRSPERERLFALWTLGQLTVGVALLYALPRLFQLFGL
                     QVLYLLIAALMVASTPLLKPLPAHVQAPARPQPGSRTSLWGAAPGLASVLLYYIGYGG
                     IWPFLSAIARAGGSQPLASGEVLALAGLCGIAGALAAALRARQGGWGVLAPGFGAHLL
                     ALFLLLQAPALLRFAVGACLLKGASNFTLPFLLGKTARLGSDRRLMGMANMAIGGGLA
                     IGPLITGRLIELSGGYTLPISLSAGLILASALLLLPIGQASPTRPRPADPAADPAQA"
     gene            complement(4372..6711)
                     /locus_tag="VFF98_03730"
     CDS             complement(4372..6711)
                     /locus_tag="VFF98_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62772.1"
                     /translation="MPALLSHGLSGAALLVAMAPPAIAGEAAPPAASGADDIVVTAQR
                     RTQTASSLGLAITAVSGETLVEKGITSAEELWKITPGLVVKDSGYGVPNYTLRGVGFE
                     NYFVNSSSTVGIYANEIAIPYPAMSRGSFFDLERVEVLKGPQGDLYGRNTTAGQINLI
                     DRKPTRDVEAGGSLEGGSYSQVNARGYLSGPLADNVQGRLAFAIDTSDGWQHSLSRPT
                     DKPLGAKNEVALRGQINVDLGPDSSLLLRGYWNRDRSDNEALTPVDGRTLGLASAQAR
                     ATTGTVIFSTGDNRAADWSPDFRPHRDNTAIGTSATLNLARGGVKLTAISAFDSFRRY
                     EVNDWDGAAADDSNATNASRITAFSQEVRLANRSADAPVQWIAGTYFSWDRVHEDYRF
                     YMSQSYYATALGIDALNTRYTQTTRSIAGFGHVEARLADRFHLVGGLRYTHETRSFAG
                     CTYDLDGSLANFTNNILIPRVIKPAGLPVPANITQGGCSVYDDRPASPTYGTFSDAPE
                     RLSTGRWMWKGGAQFDAARDVLVYANVSSGFKSGGFNGSNANLKSQESGYSPEELTAY
                     ETGVKANLRRAHLYIDSSFFYYDYRNKQTNGVAVTFVGNITGITNIPRSRVFGIDSLV
                     RWTPLPGLSLQASGTYLDSRILRWQPVSSSSAYPTVLTYNAAGLPLPNTPRWSLSLTP
                     SYTFKVSHDRQVKLALDYYYRSSTSGIGNVYQNIPGYDLVNAEISIAPRNKRWDLTLW
                     TKNLFNRYYYNYAGLPGNADYVRLVGQPITFGVRLGFAL"
     gene            complement(7114..8139)
                     /locus_tag="VFF98_03735"
     CDS             complement(7114..8139)
                     /locus_tag="VFF98_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZU62773.1"
                     /translation="MLTVCVGALEAWQQACEDLAISHSETDRLLKTAQTQTIPLAIPL
                     AIPLAIPLADFVAMLELTATRHAGGPLGWIVGERYDFTWLGEIGQAILSSSTLGAGLQ
                     RFCDYFGLVQDATECALRQTNDTATISYRILAPQIWPRSQDALFTLSIIGQLLRRARG
                     FDWRSVQIALEGSDADLITALRQRTGVPCTGGADTNMIRFPASALGLPLLAAPDARAP
                     DYKALNRHLAQKRRAMDVELRVETEIYRLLGAEHPDHGPDQERIAANLGMSTRTLRRR
                     LAQTDRCFKGILHDCRMKQAAHEFRWHRRVSIAHTALRLGYSEHSALTRAFRSWSGVS
                     PHAFLDR"
     gene            complement(8299..9132)
                     /locus_tag="VFF98_03740"
     CDS             complement(8299..9132)
                     /locus_tag="VFF98_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU62774.1"
                     /translation="MPANLPTNLLRSFVAIVETGSMLGAADKVFLTQSALSLQIKRLE
                     QLLQLPLFHRDGRRLSLTGSGEIMVDYARRILQLQDEAINAVTEGRFAGPARIGMVQD
                     FAETLLSGLLAQFARLHPDAILYSRVAGTVELLELLERDQLDIVLGFAGPGDAHAITR
                     APMGWYGKRDLASAEEVRLAVLEAPCRFRDAAMQALDNAGRPYRITVETPNLTTLRAA
                     VEAGLGITCRTRLFCEDEPLIAADLPELPDVSCIMRTGPALDGPTDYLATLVRETVSL
                     L"
     gene            9350..10027
                     /locus_tag="VFF98_03745"
     CDS             9350..10027
                     /locus_tag="VFF98_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HZU62775.1"
                     /translation="MPTYPPILAQPGADAPLPAAGALSPAPQEAENLAPLHAFVTALG
                     ALLGPGGPQGEEAAQRIRPLLARLIARDDWLPAEFARPGAQRYQQYLLHCDSAERFSI
                     VSFVWAPGQGTPIHDHRVWGLVGVLRGQERAEHFTRAPDGSLRATGSSVLAAGEIEIL
                     SPEAGDIHRVSNALAQAPSVSIHVYGGNIGWVERAIYAPDGREQRFVSGYANSRLPNL
                     WQRPAEQ"
     gene            10024..>10693
                     /locus_tag="VFF98_03750"
     CDS             10024..>10693
                     /locus_tag="VFF98_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU62776.1"
                     /translation="MSAGEISAQAVRQALLARSEIALIDLREEADFALGHPLFAAQIP
                     LRRIALEAPWRLPRKDVPVVVYDNGEGLVAPALAQLRALGFTQGAALAGGLQGWQAAG
                     YELFEDVNSYSKAFGELVEHRRHTPSLPAPDVQALIAQKADIAILDARRFDEYATMSI
                     PGGISTPGAELVLRARAVAPDPDTTIIVNCAGRTRSIIGAQSLINAGLPNRIHALRNG
                     TIGWT"
BASE COUNT         1767 a         3315 c         3831 g         1780 t
ORIGIN      
        1 atccgcccgc ttggtggcga aacggcttga cggggccgac gaactcgtag caaccggcgg
       61 gctgcgagcg caatggagtt gaaacatatg ggttgccccg aggacgatca tcaggaaagc
      121 gccgcatccg ccaagccgcc ggtgccggaa aatgtgcagg aggcgatccg cacgctgatc
      181 cgctgggcgg gcgatgatcc gcagcgcgag ggcctgatcg acacgccggc gcgggtggcg
      241 cgggcgtggc tggaatattg ccagggctat gccgaggatc cggccgtgca tctctcgcgc
      301 cagttcgagg aagtgggcgg ctataacgag ctggtgctgc tgaaggacat ccccttccag
      361 agccactgcg agcatcacat ggcgccgatc atcggcaagg cggccatcgc ctatatgccg
      421 cgtgaccggg tggtggggat cagcaagctg gcgcgggtgc tgcatggcta tgccagccgc
      481 ctgcagatcc aggagcggct gaccgctgag gtcgcccagt gcatctggga caatctgaag
      541 ccccatggcg tggcggtggt gatcgaggcg cagcatggct gcatgaccgg gcgcggcgtg
      601 cgcacgccgg gcgtgggcat ggtgaccagc cggctgatcg gctgcttcct cgatgatccg
      661 cgcagccgca aggaagtgct cagcctgatg ggctattgat ccggcgtggc agcggccggg
      721 ctggcgcggg gcgccggccg gcccgccgcc gcccggcctt aagcctccgc aatggcggcg
      781 atggcgattg cgggcgcggg cctgccggcc gatcccctgc tggcgcccgc aagggggcgc
      841 gccaaggcag gggcatgaag gatggcgggc ggcaatcggt cgggcaggcg gcaggctctg
      901 gcggtgttct gcgcggcggg ggcgctgggc tggggcggca cggcgcaggc gcaataccgt
      961 gacaccacct ttatcgccgc cgaaggggcc gacagcggcg tttgcgacat ttacaacccc
     1021 tgcgccactt tcggctatgc gctgtatgaa acggtggccg ggggcaatat catcgtgctc
     1081 gattccggca attacggcaa tgcggtgatc gccaaatccg ttaccatcca tggcgagggc
     1141 gggcagatga cggtgatcgg cgcgctggaa gtgaacgcgg ccgcgaccga caaggtggtg
     1201 atcgacaatc tggcgttcga gggcacctat tcgggcggcg gctatgcctt cggggtgaag
     1261 gtggatctgg ccaaggatgt gctgatccag aactgccgct tcaaggatta cgacctgtct
     1321 accgacgagg aggggggcgt ggttatcgcc gggtcttcgg gcagcgtgcg gctgacggtg
     1381 aacaacagca cctttttcgc caatgccgtg ggggtgctgg tggaaagtgg cgcctcgggc
     1441 acatcgcatg ccaagatcta caattccgct ttcgtcagca acacccaggc gggcgtgcgg
     1501 gtggtgggcg ccaccaatga tgtcacgctg atgggcaatg tgctgctggc cagccccaag
     1561 gcgctggatc tgcaaagcag cggcgtggcc cgttcctatg gcaacaatgt gatcaccagc
     1621 ggcgatgcgc cctctgccct gaccatgtat tgagcggccc ggggcgaggg gcgcatggcc
     1681 ctgttgatgg cgtctagcct gccactgccg gatcggtcag gcgcgattcg tgtaggggtg
     1741 gccttttgag ggcccagggg cggatggctt ccagctggcc ggccacggtc agcaggcggg
     1801 cttcggcgcc cagggggccg gcaatctgca cgccgatcgg caacccgttc gaccatatca
     1861 gcggcacggt ggccgccggc aggcccgcgc aattgtagac cgaggtgaag attgtgtgat
     1921 gatagagcgt gtcgaaatag gcatcgagat cggttgactg catgtcgatt gtgccaagcc
     1981 tgaggggcgg gtcggcaagg gtgggggtga gcagcacgtc atatcgctcg aagaaacggc
     2041 cgaaagcgcg cgaaagctgg tggatggtgg tgaccgccca ggccagttcc tgggcggaat
     2101 ggcgccgccc ttctgccgcc cacagccagg tgatcggctc gacatcggct tctgtggccg
     2161 cgcggccaat gtgctggccg cgggtggcca gcacattggc ggcattggcc gccacgatga
     2221 tccgcgtggc gcgtttgacc gcggccagat cgatctgcgg ccttgcctcc tccacctggt
     2281 ggcccagatc gcgcagcagc agggcggtgt cctgcgcggc ggccttcacc accggatcga
     2341 ccgccgtgcc atcatggccc accgtctgca gcgcgatgcg cagcggcgag agggggcgcg
     2401 tcagggcatc gacaaagcgg cctttctggg ccggcgcggc atagggatcg ccgggctctg
     2461 cgccatgggt ggcatcgagc agggcggcac tgtcgcgcac cgatcgggtg atgacatgat
     2521 gcaccgacag gccattccag ccctcgccga tatccgggcc gaagggattg cgcccgcgcg
     2581 agggtttcag cccgaacagc ccggtgttgg ccgccggcac gcggatggag ccgccgccgt
     2641 ctgtggcatg ggccaccggc accagcccgc cggccaccgc caccgccgcg cccccgctgg
     2701 agccgccggc gctgcgcgag ggatcccagg ggttgagcgt ggggccgtag acgatggggg
     2761 cggtttcgct ggccagcccg ttttccgccg tgttggtgaa gcccagaatg acaaggcccg
     2821 ccgcgcggta gcgcccggtg atggtggaat catggggcgc gcgatgatcg gtccagaagc
     2881 ggctgccgtt cgaggtgcgc tcgccgctga gctgaatgcc ggaatccttg atgaggaagg
     2941 gcacgccggc aaaggcgccg gtggcgtgct gggcgcttct gtcgtcgaag ctggcggcct
     3001 gggccagcgc ggcgcggtcc agccggttga ccacggcatt gagctgcggg ttgcggctgt
     3061 cgatccgggc gagcgcggtc tgcaccagtt cggttgcgct gacggcccgt tcgcggacca
     3121 gttcggcaag gccggtggca tcatattgat cgtattcggc gaagagggac atggtctgga
     3181 tactccgaga ggcgggggat caggcttgcg cgggatcggc ggcgggatcg gcggggcgcg
     3241 ggcgggtggg cgatgcctgg ccgatgggca gcagcagcag ggccgaggcg aggatgaggc
     3301 cggcgctgag gctgatgggc agggtgtagc cgcccgacag ttcgatcagc cggccggtga
     3361 tcagcgggcc gatcgccagc ccgccgccga tcgccatgtt ggccatgccc atcagccggc
     3421 ggtcgctgcc cagccgggcg gttttgccca gcaggaaggg cagggtgaag ttggaggcgc
     3481 ccttgagcag gcaggcgcct accgcaaagc gcagcagcgc cggcgcctgc agcagcagga
     3541 acagtgccag cagatgcgcg ccaaagcccg gcgccagcac gccccagcct ccctgccgtg
     3601 cccgcagcgc tgccgccagg gcgccggcaa tgccgcacag gcccgccagc gccagtacct
     3661 cgccgctggc aagcggctgg ctgccgcccg cccgcgcgat cgccgagagg aacggccaga
     3721 tgccgccata gccgatgtag tagagcagca cgctggcaag gccgggcgcg gccccccaca
     3781 gcgaggtcct gctgcccggt tgcgggcgcg ccggcgcctg gacatgggcg ggcaggggct
     3841 tcagcagcgg cgtgctggcc accatcagcg cggcgatcag cagatagagc acctgcaggc
     3901 caaacagctg gaacagccgc ggcagggcat agagcagggc cacgcccacc gtcagctggc
     3961 caagcgtcca caaggcgaac aggcgctcgc gctcgggcga gcggccggcc agcgtcatgc
     4021 tcagcaccat cagcgtgccg ccggacagcg cggagatggc ccttagcggc accagccagg
     4081 caaagtgcga cacttgcgcc gaggccagat tggccaggat gaaggccgcc atcgccagcc
     4141 ccgccacctg ccgccagggc acccgcccca gccaccacag cgccggcagg gcggccaggc
     4201 acatgccgcc ctgctcaacc gtgaagtaga agccgatggc agatggcggc aggtggaact
     4261 ggctggccag ctgcgcggcg atggccggcg cggctagcag ggcggtggcg gtgatggccg
     4321 caaaggccac cacctgcgcc gcatcggccg gcaggcgatg cgttctgccg gtcacagcgc
     4381 aaagcccagc cgcacgccaa aggtgatcgg ctggccgacg aggcgcacgt aatcggcatt
     4441 gcccggcagg ccggcataat tgtagtaata gcggttgaag aggtttttcg tccacaaggt
     4501 gagatcccag cgcttgttgc gcggggcgat cgagatttcg gcgttgacga ggtcgtagcc
     4561 ggggatgttc tgatagacat tgccgatgcc cgaggtgctg gagcgatagt aatagtccag
     4621 cgccagcttc acctggcggt catggctgac cttgaacgtg tagctggggg tgagcgacag
     4681 cgaccagcgg ggggtgttgg gcagcggcag gccggcggcg ttgtaggtga ggacggtggg
     4741 ataggcgctg ctggagctga ccggctgcca gcgcaggatc cggctgtcga gataggtgcc
     4801 gctggcctgc agcgaaaggc cgggcagcgg tgtccagcgc accagcgaat cgatgccgaa
     4861 cacgcgggag cgcgggatgt tggtgatgcc ggtgatattg ccgacgaagg tcaccgcaac
     4921 gccgttcgtc tgcttgttgc gatagtcgta atagaagaac gagctgtcga tatagaggtg
     4981 ggcgcggcgc aggttggcct tcacgccggt ttcataggcg gtcagctcct cggggctgta
     5041 tcccgattcc tggctcttga gattggcatt ggagccgttg aacccgcccg atttgaagcc
     5101 cgatgacacg ttggcataga ccagcacatc ccttgccgca tcgaattgcg cgccgccttt
     5161 ccacatccag cggccggtgc tcagccgttc gggggcatcg ctgaaagtgc cataggtggg
     5221 ggaggcgggg cggtcgtcgt agacgctgca gccgccttgc gtgatgttgg ccggcactgg
     5281 caggccggcc ggcttgatca ccctggggat gaggatgttg ttggtgaaat tggcgagcga
     5341 gccgtcgagg tcgtaggtgc atccggcaaa gctgcgcgtc tcatgcgtgt agcgcaggcc
     5401 gcccacgagg tggaagcgat cggccagcct tgcttccaca tggccgaagc cggcgatgct
     5461 gcgggtggtt tgcgtgtagc gcgtgttcag cgcatcgatg cccagcgccg tggcataata
     5521 ggattgcgac atgtagaagc ggtagtcctc gtggacgcga tcccacgaga aataggtgcc
     5581 ggcgatccat tgcaccggcg catcggccga acggttggcc aggcgcactt cctgcgagaa
     5641 ggcggtgatc cggctggcgt tggtggcgtt ggaatcgtct gccgcagcgc cgtcccaatc
     5701 gttcacctca tagcggcgga agctgtcgaa ggcgctgatc gcggtcagct tcacgccgcc
     5761 ccgggcgagg ttgagcgtgg ccgaggtgcc gatagcggta ttgtcgcggt gggggcgaaa
     5821 atccgggctc cagtcggctg cgcggttgtc gccggtggag aagatcaccg tgccggtggt
     5881 ggcgcgcgcc tgtgccgagg caagccccag cgtgcggccg tcaaccggcg tcagcgcctc
     5941 gttgtcggag cggtcgcggt tccagtagcc gcgcagcagc agcgaactgt cgggccccag
     6001 atcgacgttg atctgcccgc gcagggccac ttcattcttg gcgcccagcg gcttgtcggt
     6061 ggggcgcgag aggctgtgct gccagccatc ggaggtgtcg atggcaaagg cgaggcggcc
     6121 ctgcacattg tcggccagcg ggccgctgag atagccgcgc gcgttgacct ggctgtagga
     6181 gcccccctcg agcgagccgc ccgcctcgac atcacgggtt ggcttgcgat cgatcaggtt
     6241 gatctgcccg gcggtggtgt tgcggccata cagatcgccc tgcggccctt tcagcacttc
     6301 cacgcgttcc aggtcgaaaa agctgccgcg gctcatcgcc ggatagggga tggcgatttc
     6361 attggcgtat atgccaacgg tgctggacga attgacgaag tagttttcaa aaccaacgcc
     6421 gcgcaaggtg taattgggca cgccatagcc cgaatccttg accaccagcc ccggcgtgat
     6481 cttccacaat tcttcagccg aagtgatgcc tttctcaacc agtgtttcgc ccgaaacggc
     6541 ggtgatcgcc aggcccaggc tgctggcggt ttgcgtgcgg cgctgcgcgg tgacaacgat
     6601 gtcgtccgcg cctgaggcgg cgggcggggc ggcttctccg gcgatggcag gcggcgccat
     6661 cgccaccagc agcgcggccc ccgacaggcc gtggctgagg agcgccggca tggcccggcg
     6721 ccccttcgcc cggccgttgg cagcgtttgt ccgatattgc tgtcgttcat tcatgtcaga
     6781 tctggcccct gtctttgcgt ctgtttcgcg caatacgaag aaaagcgatg tggcttcgct
     6841 gtttccgggg gcgacgatgg caaggtgaat ggccagaggc ttgacatgat cggccaaaac
     6901 attctggcat attgcctgtt gtttttgtct gatcgagaaa tttgtcgatg gtataaattt
     6961 atatcaacag ctgtttgtga attttcatag gttatatttc gaagtataaa tatctcttct
     7021 gccgttatga cttttgctga taggccccaa ccacacatgg agatcgccgt gctttcggca
     7081 tcgttccacg aaaagggagc gttcctgcca gccctagcgg tcgaggaagg cgtgggggga
     7141 aacgccgctc cagctgcgga acgcgcgggt cagcgcgctg tgttcggaat agcccagccg
     7201 cagcgcggta tgggcgatcg acacgcggcg gtgccagcgg aactcgtgcg cggcctgctt
     7261 catgcggcaa tcgtgcagga tgcccttgaa gcagcggtcg gtttgcgcca gccgccggcg
     7321 caaggtgcgg gtggacatgc ccagattggc cgcgatgcgt tcctgatccg ggccatgatc
     7381 ggggtgttcg gcgcccagca ggcggtagat ttccgtttcc acccgcagtt ccacatccat
     7441 ggcgcggcgt ttctgggcca ggtggcggtt cagcgccttg tagtcggggg cgcgcgcatc
     7501 gggcgcggcc agcagcggca gccccagcgc cgaggcggga aagcggatca tgttggtatc
     7561 cgccccgccg gtgcagggca cgccggtgcg ctggcgcagg gccgtgatca gatcggcatc
     7621 gctgccttca agggcaatct gcaccgagcg ccagtcgaaa ccacgcgcgc gccgcagcag
     7681 ctggccgatg atcgacaggg tgaacagcgc atcctggctg cggggccaga tctgcggggc
     7741 gaggatgcgg tagctgatcg tggcggtgtc gttggtctgg cgcagcgcgc attcggtggc
     7801 atcttgcacc aggccgaaat aatcgcagaa gcgctgcagg cctgcgccca gggtagagct
     7861 ggagagaatg gcctggccga tctcgcccag ccaggtaaaa tcatagcgtt cgcccacgat
     7921 ccagcccagc gggccaccgg catggcgggt ggcggtcagc tccagcatgg ccacgaaatc
     7981 ggctagggga atggccaggg gaatggccag gggaatggcc aggggaatgg tttgcgtctg
     8041 ggccgttttc agcagccggt cggtttcgct gtggctgatg gcgaggtctt cgcaggcctg
     8101 ctgccacgcc tccagggcgc ccacgcatac tgtcagcatc agatgtggct cccggtcccg
     8161 aaatgatttg catcaaattc ctatcatata aataggtatt tggcggggaa gaaaatgttt
     8221 ggaggcatgg ctgtctggct tttggccggg cccgtggcac gggggcgtgg caacgcccgg
     8281 ctcggcgggc aggagggatc agagcaggct gaccgtctcg cgcaccagcg tggcaaggta
     8341 atcggtgggg ccatcaagcg ccgggcccgt gcgcatgatg caggacacat cgggcagttc
     8401 gggcaggtcg gcggcgatca gcggctcgtc ctcgcagaac aggcgggtgc ggcaggtgat
     8461 ccccagcccc gcctccaccg ccgcgcgcaa ggtggtgagg ttgggcgtct cgacggtgat
     8521 gcgatagggc cgcccggcgt tgtccagcgc ctgcatggcg gcatcgcgaa agcggcaggg
     8581 ggcttccagc acggccagcc gcacttcctc ggcgctggcc agatcgcgct tgccatacca
     8641 gcccatgggc gcgcgggtga tggcatgggc atcgcccggg ccggcaaagc ccagcacgat
     8701 gtcgagctgg tcgcgctcca gcagttccag cagttccacc gtgccggcca cgcgcgaata
     8761 gaggatggca tcgggatgca gccgggcaaa ctgcgccagc aggcccgaca gcagggtctc
     8821 ggcgaaatcc tgcaccatgc cgatgcgcgc gggcccggca aagcggcctt ctgtcaccgc
     8881 attgatcgcc tcatcctgca attgcaggat gcgcctggca taatccacca tgatctcgcc
     8941 gctgccggtg agcgagaggc ggcgcccgtc gcggtggaac agcggcagct ggagcagctg
     9001 ttccagccgc ttgatctgca ggctgagcgc cgattgggtg aggaacacct tgtcggcggc
     9061 gcccagcatg gagccggtct caacgatcgc cacgaagctg cgcagcaggt tggtcggcag
     9121 gttggcaggc atcgctctcg cttcatccgg ggggccctgg ctggcctgca taaggcaggg
     9181 ccttatatcc tatcaattta attgagattc ttccggggca agtttttctt gccgggccag
     9241 cccgccccgg cagctgatga gcgctgcgca taagcggctg gcagaactca tttgatcggc
     9301 ccggatcaac gcgctttgct ggccgcctca gccgacagga gagcgcatga tgcccacata
     9361 tccccccatt ctcgcgcagc ccggggcaga cgcgccattg cccgcggcag gcgcgctatc
     9421 gccagccccg caggaggcgg aaaacctggc ccccttgcac gccttcgtca ccgcgctggg
     9481 cgccctgctg gggccaggcg ggccgcaggg cgaggaagcg gcgcagcgca tccgcccgct
     9541 gcttgcccgg ctgatcgccc gtgacgactg gctgccggcc gaattcgccc ggcccggcgc
     9601 gcagcgctat cagcaatatc tgctccattg cgacagtgcc gagcgtttca gcatcgtctc
     9661 cttcgtctgg gcgccggggc agggcacgcc catccacgat catcgcgtct gggggctggt
     9721 aggcgtgctg cgcggccagg agcgggccga gcatttcacc cgcgcgcccg atggcagcct
     9781 gcgcgccact ggcagcagcg tgctggcggc cggcgagatc gagatcctgt cgcccgaggc
     9841 gggcgatatc caccgggtca gcaatgcgct ggcgcaggcg ccttcggtgt cgatccatgt
     9901 ctatggcggc aatatcggct gggtggagcg ggctatctac gcgcccgatg gccgcgagca
     9961 gcgctttgtc tcgggctatg cgaacagccg cctgcccaac ctctggcaaa ggcccgccga
    10021 gcaatgagcg ccggggagat ctctgcgcag gccgtgcgcc aggcgctgtt ggcgcgcagc
    10081 gaaatcgccc tgatagacct gcgcgaggaa gccgatttcg ccctgggcca tccgctgttc
    10141 gccgcgcaaa tcccgctgcg ccggatcgcg ctggaggccc cctggcgcct gccgcgcaag
    10201 gacgtgccgg ttgtcgtcta cgacaatggc gaggggctgg tggccccggc gctggcgcag
    10261 ttgcgggcgc tgggtttcac gcaaggggcg gcgctggccg gcggcctgca gggctggcaa
    10321 gcggccggct atgagctgtt cgaggatgtg aacagctatt ccaaggcctt tggcgagctg
    10381 gtcgaacacc gtcggcacac gcccagcctg cccgcgcccg atgtgcaggc gctgatcgcg
    10441 caaaaggccg atatcgccat cctcgatgcc cgccgcttcg atgaatatgc caccatgagc
    10501 attcccggcg gcatttccac gcccggcgcc gaactggtgc tgcgcgcgcg ggccgtggcg
    10561 cccgatcctg acaccacgat catcgtcaat tgcgcgggcc gcacccgctc gatcatcggt
    10621 gcccagtcgc tgatcaacgc cggcttgccc aaccgcatcc atgcgctgcg caacggcacc
    10681 atcggctgga cgc
//
LOCUS       DASRYE010000068        27105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_233273,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000068 DASRYE010000000
VERSION     DASRYE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233273"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFF98_03755"
     CDS             complement(<1..1080)
                     /locus_tag="VFF98_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZU62777.1"
                     /translation="MVSLVNTVAYLGLEARAVEVQCQLSAGLPGFHLVGLADKAVAES
                     RQRVQAALSAMGLALPPKRITVNLSPADLPKEGAHYDLPIALALLAAMDVVDGEALAD
                     HMVVGELALDGRLAPGAGVLLAALHAAGLGKGLICPADQGAEAGWVGDVPVLAAPDLV
                     SLLNHLRGESGLPPPPAPPPVESAVSAPCLSGVKGQEVARRALEVAAAGGHNLLMCGP
                     PGAGKSLLAACLPGILPPLSAAEALEVSMVASVAGTLAGGRISATRPFRAPHHSASMA
                     AMTGGGARPRPGEVSLAHMGVLFLDELPEFQRAVLDSLRAPLETGAVTVARANGHVTF
                     PARVQLVGAMNPCRCGHLGDAGFACG"
     gene            1294..1428
                     /gene="rpmH"
                     /locus_tag="VFF98_03760"
     CDS             1294..1428
                     /gene="rpmH"
                     /locus_tag="VFF98_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZU62778.1"
                     /translation="MKRTYQPSRLVRARRHGFRARTATVGGRNVLRARRARGRKKLSA
                     "
     gene            1530..1958
                     /gene="rnpA"
                     /locus_tag="VFF98_03765"
     CDS             1530..1958
                     /gene="rnpA"
                     /locus_tag="VFF98_03765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZU62779.1"
                     /translation="MPAPRVLTKRADFLAANNGLRVARPGFVLLVRGNGGAGLRFGIT
                     VTKRIGNAVVRNRMKRRFRALVREMLPAAGLADHDHVLIGREGGVERDFAQLRTELAA
                     ALERARAGKGDPPRKGPRGGAKATPKGRGRPSAPAPSARA"
     gene            1955..2167
                     /gene="yidD"
                     /locus_tag="VFF98_03770"
     CDS             1955..2167
                     /gene="yidD"
                     /locus_tag="VFF98_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZU62780.1"
                     /translation="MIRALLIGLIRLWQWGPSAMMGPTCRFQPSCSAYAIGALRKHGT
                     IKGGWLALKRLLRCHPWGGHGFDPVP"
     gene            2227..4014
                     /gene="yidC"
                     /locus_tag="VFF98_03775"
     CDS             2227..4014
                     /gene="yidC"
                     /locus_tag="VFF98_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZU62781.1"
                     /translation="MNNQRNLILAMLLTGLLLFGWDAAIRYLYPNASKPHVAAAAPSP
                     TASAKPSREGGLASPLDQAAEAQDLAADLKPAGRVTIAAPGLSGSINLAGAVIDDLSL
                     NRHRATLAKDSPAVRLFSPAGTPAQHFAQVGWVSAPGSAGIALPGGNTVWQAAPGAKL
                     TPTTPVDLNWTNPTGQTFTIRLAIDEDYLITISQSVANRGTQQVALQPFALINRTDRT
                     ASQGGWQIHSGPFGAFDGKVSFSPNYKDVVKSGLIANDGKPDWMGFTDIYWMSVLVPA
                     ANVATSDFRDLGNSTYRADVIYQPESIAPGQSATLSTRLFAGAKEATVLDRYAEGSNG
                     QPAIAKFGYAIDWGWFWFFEKPIFALMRSLYHATGNFGIAIILLTLIVRGVMFPVAQR
                     QFASMASMKAIQPKLKALQARYKDDRQKLQEETMKLYKEEGVNPLAGCLPIFLQIPVF
                     FALYKVLMVSIEMRHAPFVAWIKDLSAPDPLHIFNLFGLIPWDPPSYLSLGVLALVLG
                     CTMWLQFRLQPAAPDPAQQQVMSLMPWMMMFVMAPFASGLLIYWITSNILTVGQQRIL
                     YARHPQMRAMVEKERADVAEAARAAHHKK"
     gene            4011..4688
                     /gene="yihA"
                     /locus_tag="VFF98_03780"
     CDS             4011..4688
                     /gene="yihA"
                     /locus_tag="VFF98_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZU62782.1"
                     /translation="MSDAEKLDQTPVDPALVEEARKLFSGRVEFLLSAPALQFLPDPV
                     VPEIALAGRSNVGKSSLLNAITGRKGLARASVTPGRTQELNFFEVGGAAGELPDFRLV
                     DMPGYGFAKAPPAVVEKWKRLVREFLRGRVVLKRCLLLIDSRHGPKPVDIEMMQMLDE
                     AAVGYRIILTKADKVKASELAGAHAAAQAEARKHSAAFPQVAVTSSETGLGIAELRAA
                     VLGDTLV"
     gene            4739..5401
                     /locus_tag="VFF98_03785"
     CDS             4739..5401
                     /locus_tag="VFF98_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU62783.1"
                     /translation="MKLLIGNRRYSSWSLRGWLAARQAGLAFDLEVVPLFGSDPAADH
                     RAHPALAPSAGRVPILWDGDAVVWDSLAIMDYLADKVGRDRFWPKQEAARALARAMVA
                     EMHSGFAPLRRALPMDLQRPPAPLDPAACHQPDAVEGDILRVLALWAEARSRFGAGGP
                     FLFGSFGAADIAFAPVVARFHHYAVPVPGFAQAYMAAVWEHAWLREWAAGAAEETWVI
                     PG"
     gene            5419..6303
                     /locus_tag="VFF98_03790"
     CDS             5419..6303
                     /locus_tag="VFF98_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HZU62784.1"
                     /translation="MTMPGTMLGKLRWACMAAAAAAMAMAGPAMAWGNAGHRMVAEIA
                     EANVPPQVQAEIAQLLAAEAGLGTPACRVHSLADAATWPDCLRGEDWRWAYTFAWHYQ
                     DADVLGHGFDAAAHCTYGNCVTAQIARNRRILADRHLPAAQRLEALAFLAHFVGDIHQ
                     PLHAADHDDDHGGNGVKASWPGDVPSSLHWFWDKAVAERAIATAPGPMVRLYSAEERA
                     RIATGQPADWAQESWQIAHDVVYPQAFGHLPARGEAPPAMVVMSQQQLDADAPIGRQR
                     LVQAGLRLARELTAALGG"
     gene            6373..7530
                     /gene="dapE"
                     /locus_tag="VFF98_03795"
     CDS             6373..7530
                     /gene="dapE"
                     /locus_tag="VFF98_03795"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZU62785.1"
                     /translation="METLVKAGADVAELAEALIACPSVTPAAGLVFDCLEQMLAPLGF
                     AVHRFLAGEAPDGPVENLFAIRQGPEGSRHFAFAGHVDVVPPGEGWTSAPFAPERRGP
                     KGEELLYGRGAVDMKGAIASMVAAVRTVPAEAGTISFIITGDEEGPARFGTLALMDHM
                     RARGLCPDLILVGEPTSVSRLGDMIKVGRRGSVNIWLEVEGQQGHVAYPHLADNPLTR
                     LVALLAELKAMVLDEGTEWFQPSNLEITDVGCGNPATNVIPARASARISIRFNDRHTG
                     ESLADRVSAIAARHGASARAVISGEAFLTPPGAFSEALRTAIHAETGLDAEVSTSGGT
                     SDARFLKDFAPVIEFGLTNATMHKRDEAVAVADLAALARIYAAVTRAGLAG"
     gene            complement(7527..8204)
                     /gene="nth"
                     /locus_tag="VFF98_03800"
     CDS             complement(7527..8204)
                     /gene="nth"
                     /locus_tag="VFF98_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZU62786.1"
                     /translation="MKKADIFEFFRRLAEANPDPQTELAFGNVYQLLVAVTLSAQATD
                     VGVNKATRALFEIVKTPQDMLDLGEEGLKSHIKTIGLFNAKAKNVMAMAAILVRDYGG
                     EVPADRAALETLPGVGRKTANVVLNCAFGAETFAVDTHIFRVGNRTGLAKGKTVLAVE
                     KGLERHVPQPFRVGAHHWLILHGRYICKARRPECWRCPVADLCAYKPKEAEPAKGKAK
                     ASAKAKT"
     gene            complement(8247..9140)
                     /locus_tag="VFF98_03805"
     CDS             complement(8247..9140)
                     /locus_tag="VFF98_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZU62787.1"
                     /translation="MNGGLLRVDLAVCTYRRWSLMECLESIAAQDMRRLVDLRVIVAD
                     NNESPMMAEAVAHAARDLALPITYVHAPAKNISVARNACLDAAEGDWLLFIDDDEAAR
                     PNWLARLLARREEADVIFGVSEALMGAGAPGWMRSGAFHSNRFNGNDKPHNGYTCNVA
                     MRRAAIAGLRFAPELGTVGGEDTLFFHEAMRRGLRFAYAPDAVVTEHIPDHRATLGWL
                     LRRRFRSGQTHQMLLAREGRRAITVVPVAVFKVACCLLAALANGFSRERAAAFLARGW
                     FHCGVVAAALGRRHVQEYAAA"
     gene            complement(9248..9694)
                     /locus_tag="VFF98_03810"
     CDS             complement(9248..9694)
                     /locus_tag="VFF98_03810"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZU62788.1"
                     /translation="MSVGVEREALVAAALAASAAAYAPYSRFHVGAALAFADGTVLAA
                     ANVENASYGLSLCAETVAVARAMAEGRRGGLLAVAVAGGPAGPDGQAVPGAAPVSPCG
                     RCRQVLSELAQLGGTDPVVWSVHAGGVAEHRLSALLPLAFGPANLG"
     gene            complement(9691..10416)
                     /locus_tag="VFF98_03815"
     CDS             complement(9691..10416)
                     /locus_tag="VFF98_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HZU62789.1"
                     /translation="MAKQNRRRTGSARSRGGWRPRLAALALLLVLAGAAWGWWSLRHW
                     QPARGLYPIQGVEMGAGDGVVDWTAIHAIDANFAYVETSAGAFARDPAAERNLEGARA
                     AGLKVGAVHRYDPCQPADRQAANFVTVVPRDARMLPPAIALEDLADSCPVAVSDAAVL
                     SELMTFINEIETHTGKPAILAIGPDFEARYHLSGRINRELWLRRDRLRPSYAGRPFTL
                     WTANSALMTSAGDAPLRWVVAQP"
     gene            complement(10448..10930)
                     /locus_tag="VFF98_03820"
     CDS             complement(10448..10930)
                     /locus_tag="VFF98_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HZU62790.1"
                     /translation="MADARISHIELDEATILWRSADIEQERRIAIFDLIEENSFKPLR
                     AFGAGHEGPYRLRLAVADGRLAMDIAEESGQLLETLVLGLGRFRRPIRDYFAICDSYY
                     QAIRKATAQEIETIDMARRGVHNGAAELLLERLDGKVETDFPTARRLFTLICVLHIRG
                     "
     gene            11090..12622
                     /locus_tag="VFF98_03825"
     CDS             11090..12622
                     /locus_tag="VFF98_03825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZU62791.1"
                     /translation="MAQIEPLLSLAGTAAEPPAPRALPANIEAEAGFLGAVLIDNRVT
                     EELNTPLAPAHFFEPVHGRIYERILQLLDRKAVVTPVTLRPYFEADEALRALGGPAYL
                     ARLTADGQGLIAPKVLAEQIYDLALLRELVRVGRELIDGAMDTSEAVEPLVRIEQAEA
                     ALYQVAEGAGPSQEAASFAAATRVAIQGIEKAFNSGGHISGKTTGLTSVNDRIGGLHD
                     SDLIILAGRPGMGKTSLVTNIAFNTADRYMRDLADGITPEQSVGAPVAFFSLEMSSDQ
                     LATRILAEQSGISSESLRMGKISRQDFHELANASQRLASLPLYIDDTPGLSIAALRAR
                     ARRLKRRHNIGLVVVDYLQLLQGTNRSGNDNRVNEISEISRGLKTLAKELHLPVIALS
                     QLSRAVEQREDKRPMLSDLRESGSIEQDADMVWFVFREDYYVKAKEPKEPSPTDPQPI
                     HDAHMKWAEEMERVNGLAELIVAKQRHGATGKVRLRFESRITKFSDLAPDDLRQNVYD
                     DE"
     gene            12721..13065
                     /gene="rpoZ"
                     /locus_tag="VFF98_03830"
     CDS             12721..13065
                     /gene="rpoZ"
                     /locus_tag="VFF98_03830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZU62792.1"
                     /translation="MARVTVEDCVDKVPNRFDLVLLAAQRAREISGGAELTLERDRDK
                     NPVVALREIAEETVTPRMLKEALITSMQRVLPDDDDEVDEIGSLSQQAEALRITAAAP
                     VRNTSIGGDYDG"
     gene            complement(13164..15116)
                     /gene="ftsH"
                     /locus_tag="VFF98_03835"
     CDS             complement(13164..15116)
                     /gene="ftsH"
                     /locus_tag="VFF98_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZU62793.1"
                     /translation="MNEDQPNGSPWMKSLLLWGGIVLAVLFAVSLFGSRADMGSSQIP
                     YSDFRAKVAEGGVADAQVGEQRITGHYKNGDAFSTVPIPNDTSLPQLLQQAGVKYAGK
                     APDEPNVLVYVLVQALPFMLILAVAFFALRQVQKGGGGGAMGFGKSKAKLLTERAGRV
                     TFADVAGIDEAREELEEIVEFLRDPGRFSKLGGQIPKGALLVGSPGTGKTLLARAIAG
                     EAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGHGLG
                     NSNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQVVVPVPD
                     IEGREQILAVHMKKVPLAPDVEARVIARGTPGFSGADLANLVNEAALLAARRSKRLVA
                     MQEFEDAKDKVMMGAERRSMVMTEEEKKMTAYHEAGHALVSLNEPASDPIHKATIIPR
                     GRALGMVMRLPERDSYSYHRDKMHANLSVAMGGRVAEEIIFGYDKVSSGASGDIQYAT
                     GLARNMVTKWGMSDKLGPLQYEDQQEGYLGMSGSQRLMMSEDTSRLIDSEVRALVDGA
                     HVRATGILKTQEDKLHALAGALLEYETLTGEEIRQVVDTGRLDRPDQPASGTQRPAAI
                     AGTSVPRAGRRFLGGMGGAMPAPLGA"
     gene            complement(15237..15842)
                     /gene="metW"
                     /locus_tag="VFF98_03840"
     CDS             complement(15237..15842)
                     /gene="metW"
                     /locus_tag="VFF98_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZU62794.1"
                     /translation="MNRVSVALRPDLAVIAAHVARAARVLDVGCGDGTLMAALRDERA
                     CDARGLELDAHNAGLCVEKGLSVIQGDADTDLALYPDDSFDYAILSQTLQTTKRPDLV
                     LAELLRIGRRAFVSFPNFAHWRVRMSLLVGGHMPVTRLLPVAWYETPNIHHLTIADFR
                     DLVRAKGLVVEQAWFLKGDALIGPALANLRAEHAVFLLSRG"
     gene            complement(15853..16983)
                     /locus_tag="VFF98_03845"
     CDS             complement(15853..16983)
                     /locus_tag="VFF98_03845"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZU62795.1"
                     /translation="MASAPLLTTSAALDLARPLPLDGGQSLAGVRIAYETYGELAADG
                     GNAILICHATTGDQHVASPHRITGKPGWWARMVGPGKPIDTNRFFVICPNVLGSCMGS
                     TGPASPGPDGRPYGMRFPVITIRDMARAHVALLEALGVERLYAVVGGSMGGMQALSLA
                     ANWPHIPERVLALACTASMPAQNIAFQEVGRQAIIADPDWQGGDYYNTGRSPDAGLAV
                     ARMAAHITYLSEATLAEKFGRRLQNRDAISFGFGADFQVESYLRYQGSSFTGRFDANS
                     YLYITRAMSYFDLAEERFGPGAHGQGRLAEAFAGTSARFCFVSFDTDWLYPTAESRKL
                     AHALNAAGAPVSFMELAAPFGHDSFLLDVPALDRVIAGFLSR"
     gene            17097..18209
                     /gene="hisC"
                     /locus_tag="VFF98_03850"
     CDS             17097..18209
                     /gene="hisC"
                     /locus_tag="VFF98_03850"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZU62796.1"
                     /translation="MTQNPRAQAPAPKPWISQIHAYVPGKSKGHDGRPLIKLSANENP
                     LGTSAVALAARAEAAAPALYPDPDSVALRTAIATLHGLAAERVVMGTGSDELLHIAAQ
                     AYAGPGDEILYVRYGFSVYDIAARRCGAVPVVAPDADYGTDVDALLALVNEKTRVVFV
                     ANPNNPTGSFLAHGEIARLHAGLPADVLLVVDQAYGEYVAPEDDDGALALAEAHQNVL
                     VTRTFSKIYGLAGERLGWATGAPALIDTLNRIRGPFNLSNTAQAMGLAALADQAFVES
                     SRQHNTAERARFAEKLAALGNHGIRPLPSQANFVLILFAGALSAEAAYEGLMAAGYIT
                     RWLPNQGLPQALRITIGTAAQMDEIAAALTAMASGQ"
     gene            18206..19168
                     /locus_tag="VFF98_03855"
     CDS             18206..19168
                     /locus_tag="VFF98_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HZU62797.1"
                     /translation="MSPPTSDRALSDEAPFRKVAIIGLGLQGGSIGLALAAALPAIAT
                     TGYDLSAANRQRAAERGLVGQVCATAPEAVTGADLVIFCVPPGAMGAAAAEVAGALAP
                     DAIVSDVGSSKVLIGAALAEALPAAAAAGRVIPAHPVAGTENSGPDAGFATLFRQRWC
                     IITPPPHVDPAGLARLVALWEAIGARVELMEPAHHDRVLAVTSHLPHLIAYTIVGTAG
                     DLEEVTQSEVIKYSAGGFRDFTRIAASDPTMWRDVFLSNREAVLEMLQRFTEDLTELQ
                     KAIRLGDGAKLFDHFTRTRAIRRSIIEQGQDDARPDFGRSDHAR"
     gene            complement(19197..19913)
                     /locus_tag="VFF98_03860"
     CDS             complement(19197..19913)
                     /locus_tag="VFF98_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU62798.1"
                     /translation="MNPSPTGEAARLSDLLRSLLFYPAFYGITVGMALFIPLVLVLPR
                     GATLRWCSWWAAGQRWCMRWIMGVKVVVEGPMPTRPVLVAMKHESFFEAIDLPVLFRF
                     PVVFAKVELLRLPLWGKAGLRYGLIPVERDQGARALRAMVAAARASVGEGRHLVIFPE
                     GTRVPHGGPAPLQAGFAGLYKLIGLPVVPVAVNSGPLYHRWIKRSGTLIYRFGAEIPP
                     GLPRDEVEARVQAAINALNG"
     gene            complement(19921..20454)
                     /locus_tag="VFF98_03865"
     CDS             complement(19921..20454)
                     /locus_tag="VFF98_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZU62799.1"
                     /translation="MLRRLLALLLVAAVLGFLAFAVTLPRPAGGQPTDAVIVLTGGEG
                     RIPRALDVLRRGWAKRLLVSGVDSQVTAGEFAARYQVSAATMACCITLGYESVDTRSN
                     AREAANWIAAHHARSLRLVTSDWHMRRAAWELSRTIPADVAVVEDAVPTHPSLNMLMV
                     EYSKYLARRLLRLAHRG"
     gene            complement(20458..21405)
                     /locus_tag="VFF98_03870"
     CDS             complement(20458..21405)
                     /locus_tag="VFF98_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HZU62800.1"
                     /translation="MNGFSPAREAAGAPGPAAPPPGRWRRSGRDMLPPGRLSGPMPWM
                     IAIMVGLTVLAAGSGLALRHIAVAAGGALGHGVSVQILEPRADLRQQQAAAVLAALKA
                     EPGVLAARQVPEAELDALIAPWLGGENDPALLPSPALIDVTLDGPASASRLAALRAAA
                     AGHAPQLEVDTQASWLAPVFGAIASLQWLAAGLVVLLGAALCAAVLLAVRTGLGNHRA
                     TLEIVHLLGGTDRQIARVFQRSIALDAALGGAVGLAGGAGALWLVGTRFGVLGAGLVS
                     DGALAWPDWLALAGVPVAGVLLAMATARLTVLAALKKML"
     gene            complement(21402..22142)
                     /gene="ftsE"
                     /locus_tag="VFF98_03875"
     CDS             complement(21402..22142)
                     /gene="ftsE"
                     /locus_tag="VFF98_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZU62801.1"
                     /translation="MVSAPPQTGEIVHFDNVGLRYGSSDPEVLSDLGFSLYAGSFYFL
                     TGASGAGKTSLLKLLYLSQRPTRGLVRLFGIDGVTLPRERLPGLRRRIGVVFQDCRLV
                     PHLSAFDNVALPLRVAGVPESEVARPVADMLEWVGLAGRMQALPATLSGGEQQRVAIA
                     RAVIGRPELLVADEPTGNVDPEMAVKLLRLFEALNRLGTTVVVATHDVHLLHQVQDSL
                     IMRLDRGRLADPTGALRYPPRRPGVGAP"
     gene            22330..23172
                     /locus_tag="VFF98_03880"
     CDS             22330..23172
                     /locus_tag="VFF98_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZU62802.1"
                     /translation="MIIACPACATRYALPDEAIGAAGRTVRCAKCRHSWFQSGPEQPA
                     PPPPAAPVAEPAAPPPSAPPRPAAAVAEAARMPSPEQLREALARTMAAATASQAIPAS
                     ILPTAPAPAEVAESAPAAETPADEPSYFEHEPPFRPRRNPARLWTAAALIFALLSCAA
                     IAAVARFGLPDWVPLATSGFGPAQPDLALSFPPNRQDRRTLPDGTQFFGITGTVTNIG
                     QQRRAVPTLLILLRDDKNRVVYSWEVTAPKRVLAPGESVPIIEAATDIPQTAKFAEIG
                     WKPA"
     gene            23312..23398
                     /locus_tag="VFF98_03885"
     tRNA            23312..23398
                     /locus_tag="VFF98_03885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23346..23348,aa:Leu,seq:gag)
     gene            complement(23540..25153)
                     /locus_tag="VFF98_03890"
     CDS             complement(23540..25153)
                     /locus_tag="VFF98_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62803.1"
                     /translation="MPENTGFALSRRGLLGGAAALAGLPLLEACAGKAGAGNTLIYGI
                     VNDPAFLVAAITTAGEAQWLSPKIADGLLNYTFDLKPRPGLAQAWDFSADSRALTLKL
                     RPGVRWHDGRDFTSHDVAFSALHLWKQFHSRGRMTYAHLAGVDTPDPLTAILRFDKPS
                     PYVISALAANESQVAPRHLYEGRDVLSNPANIAPVGNGPFRFAEWQRGQFLRLERNPD
                     YWDKGKPHVDGIILRILGDSAALSTALETGEIQFTPGVPNGDYQRLAKLPNLRADDRL
                     FPLATSGTGIEFNLDVPKLQDVRVRQAVAHAIDKTFILHTILQGGGVIDKGPISQIYK
                     DFYTDDVPEYPYDPAKAIALLDAAGLKPDASGVRLRFRLSPNSAGGNNDTAKVADYIR
                     SALARVGIAVQVESLDFATFVRRVYTDRDWEVLVNSGQMGPDPVIGMQRWYWSKSFKR
                     GVAFCNGSHYNSPAADHLLETAQVETDPARRRALYAAFSQLVMTDLPRIPLYTSSGGG
                     RLVYSRQLSPLPDTAAGTFGSFADLSLSG"
     gene            complement(25184..26521)
                     /locus_tag="VFF98_03895"
     CDS             complement(25184..26521)
                     /locus_tag="VFF98_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62804.1"
                     /translation="MTQLAIAARPEPLADAVAPSSGQIAQGWGQGPSARYEALAAPFR
                     PLFRRIAEGAVVRDAERRLPFDELEWLKKAGFTRLRLPVALGGLGVSLPELHNLLIEL
                     SAADPNITNILRPHIGFTEDLLNTAHADYARRWLDEIAAGKTFGNGFSELAGNVGTTS
                     ARLVREGEGWRLNGQKYYTTGTLFADWINMGAVDEHGTPKGAVVPRRAPGVEVPDDWN
                     GFGQTLTASGSTLFRDVYLTDDLVKPETKRARYVGGLFQLIHIATLAGIGRAVADEGA
                     QAVAARSRTYGHGNAAEARQDPQVLAVVGRARGGAYAAGAITLKVAEALERVYEANRA
                     GLPAAEEAAGAIADLEINEAVSVVSELVLDAATTIFDALGASGTGRALGLDRHWRNAR
                     TITSHNPRIYRDRIVGAFAVNGTLPVAFGQAPQGGGQEADAQEARNAAAPLAL"
     gene            complement(26573..>27105)
                     /locus_tag="VFF98_03900"
     CDS             complement(26573..>27105)
                     /locus_tag="VFF98_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62805.1"
                     /translation="DARIRLYDREGTSLRVGGNFNGVDAHLRSNGSFVTGTPPWTAGW
                     NIDAATPVGRDEQKLRLTVQYSLTGGSYLTSNVAPYSTVVLSSSSAGNVYGAGVLKNG
                     ELDRLAFKIQYERPHLRNLKVWFSGVVYSGDRFAEMATTSTGFFNNTYTQRGAVYRVA
                     NAQAPFQAEGGVSIDF"
BASE COUNT         4414 a         9633 c         8889 g         4169 t
ORIGIN      
        1 cccgcaggca aagccggcat cgcccagatg gccgcagcgg cacgggttca tcgcccccac
       61 cagctgcacc cgggcgggaa aggtgacatg gccattggcc cgcgccaccg tcaccgcgcc
      121 ggtttccagc ggggcgcgca gcgaatcgag cacggcccgc tggaattcgg gcagttcgtc
      181 gaggaacagc acgcccatat gcgccaggct cacctcgccc gggcgggggc gggcgccgcc
      241 gccggtcatc gcggccatgc tggccgaatg atggggcgcg cgaaacggcc gcgtggcgct
      301 gatgcgcccg cccgccagcg tgcccgcaac cgaggccacc atcgacactt ccagcgcctc
      361 tgccgccgaa agcggtggca aaatgcccgg caggcaggcg gccagcaggc ttttgccggc
      421 ccccggcggc ccgcacatca gcagattgtg cccgccggcc gccgccactt ccagcgcccg
      481 gcgcgccact tcctgcccct tcacgcctga caggcagggg gcgctgacag cgctttccac
      541 aggtggcggg gcgggcggcg gcggcaggcc gctttccccc cgcaaatggt tgagcaggct
      601 gaccagatcg ggcgcggcca gcaccggcac atcgcccacc cagccggcct cggcgccctg
      661 atcggccggg cagatcagcc ccttgcccag ccctgccgca tggagcgctg ccagcagcac
      721 gccggcgccc ggcgccagcc gcccatccag cgccagttcg cccaccacca tatgatcggc
      781 cagcgcctcg ccatccacca cgtccatcgc cgccagcagg gccagcgcga tcggcaggtc
      841 gtaatgggca ccttccttgg gcagatcagc cggcgagagg ttgacggtga tgcgcttggg
      901 cggcagcgcc agccccatcg ccgacagcgc cgcctgcacc cgctggcggc tttcggccac
      961 cgccttgtcg gccagcccca ccaggtggaa accgggaagc ccggccgaaa gctggcattg
     1021 cacctcgacc gcgcgcgctt ccagcccgag ataggccacc gtattgacca ggctgaccac
     1081 ctgcttcccc cgcaagcccc tgaagaatag gtgtatgccc ctaatttcgc cggggttcaa
     1141 agcgcaattg cagccgggcc ccgcagcgaa tcccttgact tgaagccccc ttggccataa
     1201 tggcgcgcct tcacgaaatg gcgctggccg ctcgcggcgg cgccgcatgg gcaccggtcc
     1261 atgcaggcag acacagattt tgagggtttt gacatgaagc gcacctatca gcccagccgg
     1321 ctcgtccgcg cccgccgtca cggtttccgc gcccgcaccg ccactgtggg tggccgcaat
     1381 gtgctgcgcg cccgccgcgc acgtggccgc aagaagctgt cggcctgatt cttccttcag
     1441 ccttcgagca agaccccgcc gggccagcgc ccggtggggt ttgtgctttg gaagaggctg
     1501 aaggcgcggc tgtgccgcca gcgccccggg tgcctgcgcc cagggtgttg acgaagcgcg
     1561 ccgattttct ggcggccaac aatggcctcc gggtagcgcg gccgggcttt gtgctgctgg
     1621 tgcgcggcaa tggcggcgcg ggcctgcgct ttggcatcac cgttaccaag cgaatcggca
     1681 atgccgtggt gcgcaaccgc atgaagcgcc gctttcgcgc gctggtgcgc gagatgctgc
     1741 cggccgccgg cctggctgac cacgaccatg tgctgatcgg ccgcgaaggc ggggtggagc
     1801 gcgattttgc ccagctgcgc accgaactgg ccgccgcgct ggaacgcgcc cgcgccggca
     1861 agggcgatcc gccaaggaag ggcccgcgcg gcggcgccaa ggccacgccc aaaggccgcg
     1921 gccggccatc ggcgcccgcg ccctctgccc gcgcatgatt cgcgccctgc tcatcggcct
     1981 tatccgcctg tggcaatggg gcccttctgc gatgatgggg cccacctgcc gcttccagcc
     2041 cagctgctcg gcctatgcga ttggcgcttt gcgcaagcac ggcacgatta agggtggctg
     2101 gctggcgttg aagcgtctat tgcgctgcca cccttggggc gggcatggct tcgacccggt
     2161 gccctgacag gcccggacag gccgcctgac cctcacccat ttcagagata aacgggaaaa
     2221 cgcattgtga ataaccagcg caacctgatc ctggcgatgt tgctcaccgg gcttttgctg
     2281 ttcggctggg atgccgcgat ccgctatctc tatcccaatg ccagcaagcc gcatgtggcg
     2341 gccgccgcgc ccagccccac ggccagcgcc aagcccagcc gcgagggcgg ccttgccagc
     2401 ccgctggacc aggccgcaga agcgcaggat ctggccgcag acctgaagcc ggccggccgc
     2461 gtgacgattg cagcgccagg cctgtccggc tcgatcaacc tggccggcgc ggtgatcgat
     2521 gatctctcgc tcaaccgcca ccgcgccacg ctggccaagg acagcccggc cgtgcgcctg
     2581 ttctcgcccg ccggcacgcc cgcgcagcat ttcgcgcagg tcggctgggt gagcgcgccc
     2641 ggcagcgccg gcattgccct gcccggtggc aacaccgtgt ggcaggctgc ccccggcgcg
     2701 aagctgaccc cgacgacgcc ggtggacctc aactggacca accccaccgg ccagaccttc
     2761 accatccgcc tggcgatcga cgaggactac ctcatcacca tcagccagag cgtggccaac
     2821 cgcggcaccc aacaggtggc cctgcagccc ttcgcgctga tcaaccgcac cgatcgcacc
     2881 gccagccagg gcggctggca gatccattcc ggccccttcg gcgccttcga cggcaaggtc
     2941 agcttctcgc ccaactacaa ggatgtggtg aagagcggcc ttattgccaa tgatggcaag
     3001 cccgactgga tgggctttac cgacatctac tggatgagcg tgctggtgcc cgcggccaat
     3061 gtcgccacca gcgatttccg cgatctgggc aacagcacct atcgcgccga tgtgatctac
     3121 cagcccgaga gcattgcccc gggccagagc gccacgctgt ccacccgcct gttcgccggc
     3181 gccaaggaag ccaccgtgct ggatcgctat gccgagggca gcaatggcca gccggccatc
     3241 gccaagttcg gctatgcgat cgactggggc tggttctggt tcttcgagaa gccgatcttc
     3301 gcgctgatgc gcagccttta tcatgccacc ggcaatttcg gcatcgccat catcctgctc
     3361 acgctcatcg tgcgcggcgt gatgttcccg gtggcccagc gccagtttgc ctcgatggcc
     3421 tcgatgaagg cgatccagcc caagctgaag gcgctgcagg cccgctacaa ggacgaccgc
     3481 cagaagcttc aggaagagac catgaagctc tacaaggaag agggggtgaa cccgctggcc
     3541 ggctgcctgc cgatcttcct gcagatcccg gtgttcttcg cgctctacaa ggtgctgatg
     3601 gtctcgatcg agatgcgcca tgcgcccttt gtcgcctgga tcaaggatct ctccgcgccc
     3661 gatccgctgc acatcttcaa cctcttcggc ctgatcccct gggatccgcc aagctacctg
     3721 tcgctgggcg tgctggcgct ggtgctgggc tgcacgatgt ggctgcagtt ccgcctgcag
     3781 ccggccgcgc ccgatccggc ccagcagcag gtgatgagcc tgatgccgtg gatgatgatg
     3841 ttcgtgatgg cgccgtttgc ctcgggcctg ctgatctact ggatcacctc gaacatcctc
     3901 accgtggggc agcagcgcat cctttatgcc cgccatccgc aaatgcgcgc gatggtggag
     3961 aaggaacgcg ccgatgtggc agaggcagcc cgcgctgccc atcacaagaa gtgagcgacg
     4021 ccgaaaagct ggaccagacg ccggtggacc cggcgctggt ggaggaagcg cgcaagctgt
     4081 tctcggggcg ggtggaattt ctcctctcgg ccccggcgct gcagttcctg ccggatcctg
     4141 tcgtgccgga aatcgcgctg gcgggccgtt ccaacgttgg caagtccagc ctgctgaacg
     4201 ccatcaccgg ccgcaagggg ctggcgcgcg cctcggtcac gcccggccgc acgcaggagc
     4261 tgaacttctt cgaggtgggc ggggcggccg gcgagctgcc cgatttccgc ctggtggaca
     4321 tgcccggcta tggctttgcc aaggcgccgc ccgcggtggt ggaaaagtgg aagcggctgg
     4381 tgcgcgaatt cctgcgcggg cgggtggtgc tgaagcgctg cctgctgctg atcgattcgc
     4441 gccatggccc caagccggtc gacatcgaga tgatgcagat gctggatgag gcggccgtgg
     4501 gctatcgcat catcctcacc aaggccgaca aggtgaaggc aagcgagctg gccggcgccc
     4561 atgccgccgc ccaggccgaa gcgcgcaaac attccgccgc cttcccgcaa gtggctgtca
     4621 cctcgtcgga aaccgggctt ggcattgccg aattgcgcgc cgccgtgctg ggcgatacgc
     4681 tggtctgagg cagccccttc cccccggctg ccggtgagac agaaggatgc ccccacagat
     4741 gaagctcttg atcggcaacc ggcgctattc cagctggtcc ttgcggggct ggctggcggc
     4801 caggcaggcg ggccttgctt tcgatctgga agtggtgccg ctgttcggct ctgatccggc
     4861 ggcggatcat cgcgcccatc cggcgctggc gccttctgcc ggccgcgtgc ccatcctgtg
     4921 ggatggcgat gccgtggtgt gggacagcct ggccatcatg gactatctgg ccgacaaggt
     4981 gggccgcgac cggttctggc ccaagcagga agccgcccgc gcccttgccc gcgccatggt
     5041 ggccgaaatg cacagcggct ttgccccctt gcgccgcgcc ctgccgatgg acctgcagcg
     5101 cccccccgcc ccgctcgacc ctgccgcctg tcatcagcct gacgcggtgg aaggcgatat
     5161 cctgcgcgtg ctggcgctgt gggcagaggc acgctcgcgc ttcggggccg gcgggccgtt
     5221 cctgttcggc agctttggcg cggccgatat cgcctttgcc ccggtggtgg cgcgttttca
     5281 ccattatgcg gtgccggtgc cgggctttgc ccaggcctat atggcggccg tgtgggaaca
     5341 tgcctggctg cgcgaatggg ccgccggcgc ggcagaggaa acctgggtga tccccggctg
     5401 agacaaggac ggatgacgat gacgatgccg ggaacaatgc tgggcaagct gcgctgggcc
     5461 tgcatggcgg cggccgccgc ggcgatggcg atggccggcc cggccatggc ttggggcaat
     5521 gccgggcacc gcatggtggc cgagattgcc gaggccaatg tgccgccgca ggtgcaggcc
     5581 gagatcgcgc aattgctggc ggccgaagcc ggccttggca cgcccgcctg ccgggtgcat
     5641 tcgctggccg atgccgccac ctggcccgat tgcctgcgcg gcgaagactg gcgctgggcc
     5701 tacactttcg cctggcacta tcaggatgcc gatgtgctgg gccatggctt cgatgccgcc
     5761 gcccattgca cctatggcaa ttgcgtgacg gcgcagatcg cccgcaacag gcgcattctc
     5821 gccgatcgcc acctgccggc cgcgcaaagg ctggaagcgc tggctttcct cgcccatttc
     5881 gtcggcgata tccaccagcc gctgcacgcc gccgatcatg acgacgacca tggcggcaat
     5941 ggcgtgaagg ccagctggcc gggcgatgtg ccctccagcc tgcactggtt ctgggacaag
     6001 gccgtggccg aacgcgccat cgccaccgcg cccggcccga tggtgcggct ctattcggcc
     6061 gaggaacgcg cccgcatcgc caccggccag cccgccgact gggcgcagga aagctggcag
     6121 atcgcccatg acgtggtcta tccgcaggcc ttcggccacc tgcccgcacg cggcgaggca
     6181 ccgccggcca tggtggtgat gagccagcag cagctggatg ccgatgcccc catcggccgg
     6241 cagaggctgg tgcaggcggg cctcaggctg gcgcgggaac tgaccgcggc gctgggcggc
     6301 taaacgccca aagcaccatt ccggcagcgc tgccccttgc ggggggctgc ggttttgcta
     6361 gggccatggg ccatggaaac gctcgtcaaa gccggggccg atgtggccga actcgccgag
     6421 gcgctgatcg cctgccccag cgtcacgcca gccgccgggc tggtgttcga ttgcctggag
     6481 cagatgctgg cgccgctggg ctttgccgtg caccgcttcc tggctggcga ggcgcccgac
     6541 gggccggtgg aaaacctctt cgccatccgc caggggccgg aaggcagccg ccatttcgcc
     6601 tttgccggcc atgtcgatgt cgttcccccc ggcgaggggt ggacaagcgc ccccttcgct
     6661 ccggaacggc gcggccccaa gggcgaagag ctgctctatg gccgcggcgc ggtggacatg
     6721 aagggcgcca tcgccagcat ggtggccgcc gtgcgcacgg tgccggccga ggcgggcacg
     6781 atcagcttca tcatcaccgg cgacgaggaa ggcccggccc gcttcggcac gctggcgctg
     6841 atggaccata tgcgcgcccg gggcctgtgc cccgatctca ttctggtggg cgagcccacc
     6901 tctgtcagcc ggctgggcga catgatcaag gtgggccggc ggggcagcgt gaacatctgg
     6961 ctggaggtgg aaggccagca gggccatgtc gcctatccgc atctggccga taacccgctg
     7021 accaggctgg tggcgctgct ggccgagctg aaggcgatgg tgctggacga gggcaccgaa
     7081 tggttccagc cctcaaacct ggaaatcacc gatgtgggct gcggcaatcc ggccaccaat
     7141 gtgatccccg ccagggccag cgcgcgcatc tccatccgct tcaacgaccg gcataccggc
     7201 gagagcctgg ccgacagggt gagcgccatt gccgcccggc atggcgccag cgcgcgcgcc
     7261 gtgatctcgg gcgaggcgtt cctcaccccg ccgggggctt tttcggaagc cctgcgcacc
     7321 gcgatccacg ccgaaaccgg gctggacgcc gaggtttcca ccagcggcgg cacatcggat
     7381 gcccgcttcc tgaaggattt cgccccggtg atcgaattcg gcctcaccaa tgccaccatg
     7441 cacaagcggg acgaggccgt ggcggtggcg gatctcgccg cgctggcgcg gatctatgcc
     7501 gcggtaaccc gggcggggct ggcgggctag gttttggcct tggcgctggc cttggccttg
     7561 cccttggccg gctctgcctc cttgggcttg taggcgcaca gatccgccac cgggcagcgc
     7621 cagcattccg gccggcgcgc cttgcagatg tagcgcccgt gcaggatcag ccagtggtgc
     7681 gcccccacgc ggaagggctg cggcacatgg cgctccagcc ccttttccac cgccagcacg
     7741 gttttgccct tggccagccc ggtgcggttg cccacgcgga agatatgcgt atccaccgcg
     7801 aaggtttccg ccccgaacgc gcaattgagc acgacattcg ccgtcttgcg gcccacgccc
     7861 ggcagcgttt ccagcgcggc gcggtcggcc ggcacctcgc cgccataatc gcgcacgaga
     7921 atggcggcca tcgccatcac attcttggcc ttggcattga acaggccgat ggtcttgatg
     7981 tggctcttca gcccctcctc tcccagatcg agcatgtcct gcggggtctt cacgatctcg
     8041 aacagggcgc gggtggcctt gttcaccccc acatcggttg cctgggccga cagggtgacg
     8101 gcgaccagca gctgatagac attgccgaag gccagctccg tctgcggatc ggggttggct
     8161 tcggccaggc ggcggaagaa ttcgaagatg tcagcctttt tcatcgcccc tgtgtagagc
     8221 ggaaggcgcg cggaggcgag agcctctcag gccgcggcat attcctgcac atggcggcgg
     8281 cccagcgctg ccgccaccac cccgcaatgg aaccagccgc gcgcgaggaa agccgccgcc
     8341 ctttcccgcg agaagccatt ggccagcgcc gccagcaggc agcacgccac cttgaacacc
     8401 gccaccggca ccaccgtgat cgcccggcgc ccctcgcgcg ccagcagcat ctgatgcgtc
     8461 tggcccgagc ggaagcggcg gcgcagcagc cagcccagcg tggcgcgatg atcggggata
     8521 tgttccgtca ccaccgcatc gggcgcatag gcaaagcgca ggccccggcg catcgcctcg
     8581 tggaagaaca gcgtatcctc gccgcccacc gtgcccagtt cgggggcaaa gcgcaggccg
     8641 gcgatcgccg cccggcgcat cgccacattg caggtatagc cattatgggg cttgtcgttc
     8701 ccgttgaagc gattggaatg gaacgcgccc gagcgcatcc agcccggcgc gcctgccccc
     8761 atcagcgctt ccgacacgcc gaagatcaca tcggcctcct cgcgccgggc cagcaggcgc
     8821 gcaagccagt tcggccgggc cgcctcatca tcgtcgatga acagcagcca atccccctcg
     8881 gcggcatcga ggcaggcatt gcgcgccacc gagatgttct tggccggcgc atgcacatag
     8941 gtgatcggca gggccagatc gcgcgcggca tgggccacgg cctcggccat catcgggctt
     9001 tcgttattgt cggccacgat gaccctgagg tccaccagcc ggcgcatatc ctgcgcggca
     9061 atggattcca ggcattccat cagcgaccag cgccgatagg tgcacaccgc cagatccacg
     9121 cggagcaaac cgccgttcat tcctgccatc tccagcctag ctggcggcag tggtgcctgc
     9181 ccgcgcgcct ttttgcagcc gcagacatat ccggaatgaa tgttttgccc gcgcgggggg
     9241 cccccgctca gcccagattg gccggcccaa aggccagcgg caacaacgcc gagaggcgat
     9301 gttcggccac cccgccggca tgcacgctcc acaccacagg atcggtgccg cccagctgcg
     9361 ccagctcgct caacacctgc cggcagcgcc cgcaggggct aacgggcgcc gcccccggca
     9421 ccgcctggcc atcaggcccg gccggcccgc ccgccaccgc caccgccagc aggccgccgc
     9481 gccgcccctc ggccatggcg cgggcaacag ccaccgtttc ggcgcagagc gaaagaccat
     9541 agctggcatt ttccacattg gccgcggcca gcaccgtgcc atcggcaaag gccagcgccg
     9601 cccccacatg aaagcgcgaa taaggcgcat aggccgcggc cgaggccgcc aacgccgctg
     9661 ccaccagcgc ctcgcgctca acgccaacgc tcatggctgg gccaccaccc agcgcaaggg
     9721 ggcatcgccg gcgctggtca tcagcgcgct attggctgtc cacaaggtga aggggcgccc
     9781 ggcatagctg gggcgcaggc gatcgcgccg cagccacagc tcgcggttga tgcgcccgga
     9841 gaggtgatag cgcgcctcga aatcggggcc gatggccagg atggcgggct tgccggtgtg
     9901 ggtctcgatc tcgttgatga aggtcatcag ctcgctcagc acggcggcat cgctcaccgc
     9961 caccgggcag ctgtcggcca gatcctccag cgcgatggcc ggcggcagca tgcgcgcatc
    10021 gcgcggcacc acggtgacga aattggccgc ctgccggtcg gccggctggc acggatcata
    10081 gcggtgcacc gcgcccacct tgagcccggc cgcgcgcgcg ccttccagat tgcgctcggc
    10141 cgccggatca cgcgcgaagg cgccggccga ggtttcgaca taggcgaaat tggcatcgat
    10201 ggcgtggatc gccgtccagt ccaccacgcc atcgccggcg cccatctcca ccccctggat
    10261 gggatagagg ccgcgtgccg gctgccagtg gcgcaagctc caccagcccc aggcggcgcc
    10321 cgccagcacc agcagcagcg ccagcgccgc cagtctcggg cgccatccgc cccggctgcg
    10381 ggcgctgccg gtgcggcggc ggttttgctt ggccatcggt ttcctgtttg cttgtgctgc
    10441 gcgaatctca gcccctgata tgcagcacgc agatcagcgt aaagaggcgg cgggcagtgg
    10501 gaaaatcggt ttccaccttg ccgtccagcc gttccagcag caattcggcc gcgccattgt
    10561 gcacgccgcg ccgggccatg tcgatcgtct cgatctcctg tgcggtggcc ttgcgaatcg
    10621 cctggtaata ggaatcgcag atggcgaaat aatcgcggat ggggcggcga aagcgcccca
    10681 gccccagcac cagcgtttcc agcagctgcc cgctctcctc ggcgatatcc atggccaggc
    10741 gcccgtcggc cacggccagg cgcaggcgat agggcccttc atgcccggcg ccaaaggcgc
    10801 gcagcggctt gaagctgttc tcctcgatga ggtcgaagat ggcgatgcgc cgttcctgct
    10861 cgatatcggc gctgcgccac aggatcgtcg cttcgtcgag ctcgatatgc gagatgcggg
    10921 catcggccat gggcagtctg tcgcaaatgc gaaaggatag ggcaagaggg gcaaaggcag
    10981 gagagcggcc atggctttat ccatccccgt tatccacaag ggcaagcggc aagttcgcgc
    11041 ccgccagcca ttgccttttg cccccgcccg ggcgcatgag ggtccgctta tggcccagat
    11101 cgaacccctg ctgtcgctgg ccggcactgc tgccgagccc cccgcccccc gcgccctgcc
    11161 cgccaatatc gaggcggaag cgggctttct gggcgcggtg ctgatcgaca accgggtgac
    11221 agaagagctg aacacgccgc tggcccctgc ccatttcttc gagccggtgc atggccgcat
    11281 ttatgagcgg atcctgcaat tgctcgatcg caaggcggtc gtcacgccgg tcacgctgcg
    11341 gccctatttc gaggccgacg aggcgctgcg cgcgctgggg ggcccggcct atctcgcccg
    11401 cctcaccgcc gacgggcagg ggctgatcgc ccccaaggtg ctggccgaac agatctatga
    11461 cctggctctg ctgcgcgagc tggtgcgcgt gggccgcgag ctgatcgatg gcgcgatgga
    11521 cacatcggaa gcggtggaac cgctcgtccg gatcgagcag gccgaggccg cgctctatca
    11581 ggtggccgag ggcgccggcc ccagccagga agccgccagc tttgccgcgg ccacccgcgt
    11641 tgccattcag ggcattgaaa aggcgttcaa ttccggtggc cacatctcgg gcaaaaccac
    11701 cggcctcacc agcgtgaacg accgcatcgg cggcctgcat gattccgacc tcatcatcct
    11761 ggccggccgc cccggcatgg gcaaaacctc gctcgtcacc aacatcgcct tcaacaccgc
    11821 cgatcgctac atgcgcgatc tggccgatgg catcacgccc gaacaatcgg tgggcgcgcc
    11881 ggtggccttc ttcagcctgg aaatgagctc cgaccagctg gccacgcgta tccttgccga
    11941 gcaatcgggc atttcctcgg aaagcctgcg catgggcaag atcagccggc aggatttcca
    12001 cgagctggcc aatgccagcc agcgcctcgc ctcgctgccg ctgtatatcg acgacacgcc
    12061 cggcctttcc atcgccgccc tgcgcgcccg cgcccgccgg ctgaagcggc gccacaatat
    12121 cggcctcgtc gtggtcgact atctgcagct tttgcagggc accaaccgtt ccggcaacga
    12181 caaccgcgtg aacgagatct cggaaatcag ccgcggcctc aagacgctgg ccaaggaact
    12241 gcacctgccg gtgatcgccc tctcgcagct gtcacgcgcg gtggaacagc gcgaggacaa
    12301 gcggccgatg ctctcggact tgcgcgaatc gggctcgatc gaacaggacg ccgatatggt
    12361 gtggttcgtc ttccgcgagg attactatgt gaaggcgaag gagcccaagg agccctcgcc
    12421 taccgatccg cagccgatcc acgatgccca tatgaaatgg gccgaggaga tggagcgggt
    12481 gaacggcctg gccgagctga tcgtggccaa gcagcgccat ggcgccaccg gcaaggtgcg
    12541 gctgcgcttc gaatcgcgca tcaccaagtt cagcgatctg gcccccgacg acctgcgcca
    12601 gaacgtctac gacgacgaat gaggcccccc acgcgcgcga atccttgact ttgcccgcca
    12661 agccccctag cggctgctgt ttagccgaaa ttcccttttc ctgctgaacg gagtgcccgg
    12721 atggcgcgcg ttaccgtcga agattgtgtc gacaaggtcc ccaaccgttt tgacctcgtg
    12781 ctgcttgccg cgcagcgcgc ccgcgaaatc tcgggcggcg ccgagctgac cctcgagcgc
    12841 gaccgcgaca agaacccggt cgtggcgctg cgcgaaatcg ccgaggaaac cgtgaccccg
    12901 cgcatgctga aggaagcgct gatcacctcg atgcagcgcg tgctgcccga tgatgacgac
    12961 gaggtcgacg agatcggctc gctcagccag caggccgagg cgctgcgcat caccgcggcc
    13021 gcgccggtgc gcaacacctc gatcggcggc gattacgacg gctgatcggc aaagcgctgc
    13081 acacaaaaaa gggcggggga aaccccgccc ttttttgtgt gcccagcggc ggcgagagag
    13141 gccctcgccg ccctagccaa ggctcaggcg cccagcgggg ccggcatggc gccgcccatc
    13201 ccgcccagaa accgccgccc ggcccggggc acgctggtgc cggcaatggc agccgggcgc
    13261 tgcgtgccgc tggcgggctg gtcgggccgg tccagccggc cggtatccac cacctggcgg
    13321 atttcctcgc cggtcagcgt ttcatattcc agcagggcgc cggccagcgc gtgcagcttg
    13381 tcttcctggg tcttcaggat gccggtggcg cgcacatggg cgccatccac cagcgcgcgc
    13441 acttcgctgt cgatcagccg gctggtgtct tccgacatca tcagccgctg gctgccgctc
    13501 atgcccagat agccttcctg ctggtcctca tattgcagcg gccccagctt gtcgctcatg
    13561 ccccatttgg tgaccatgtt gcgcgccagc ccggtggcat actggatatc gcccgaagcg
    13621 cccgaggaca ccttgtcgta gccgaagatg atctcttccg ccacgcggcc acccatggcg
    13681 accgacaggt tcgcatgcat cttgtcgcgg tgataggaat agctgtcgcg ctcgggcagg
    13741 cgcatcacca tgccgagggc acggccgcgc ggaatgatgg tggccttgtg gatgggatcg
    13801 gacgccggct cgttcagcga gaccagcgcg tggccggcct cgtgataggc ggtcatcttc
    13861 ttctcttcct cggtcatcac catggagcgg cgttccgccc ccatcatcac cttgtccttg
    13921 gcgtcctcga actcctgcat ggccaccagg cgcttgctgc ggcgagcggc cagcagggcg
    13981 gcctcgttca ccagattggc cagatccgcg cccgaaaagc ccggcgtgcc acgcgcgatc
    14041 acgcgggctt ccacgtcggg cgccagcggc accttcttca tgtggacagc caggatctgc
    14101 tcgcgcccct cgatatcggg caccggcacc accacctgcc ggtcgaaacg gccggggcgc
    14161 agcagcgccg ggtccagcac atcggggcgg ttggtggcgg cgatgatgat gatgccctcg
    14221 ttggcctcaa agccatccat ctcgaccagc agctggttca gcgtctgctc gcgctcgtcg
    14281 ttgctgttgc caaggccatg gccgcgatgg cggccgacag cgtcgatctc gtcgatgaag
    14341 acgatgcagg gcgcattctt cttggcctgc tcgaacatgt cgcgcacgcg gctggcgccg
    14401 acgccgacga acatttcgac aaagtccgaa cccgaaatgg tgaagaaggg cacgcccgcc
    14461 tcaccggcaa tcgcgcgggc cagcagcgtc ttacccgtac ccggcgagcc gaccagcagc
    14521 gcgcccttgg ggatctgccc gcccagcttg gaaaaccggc ccggatcgcg caggaactcg
    14581 acgatctcct ccagctcctc gcgcgcctcg tcgatcccgg ccacatcggc gaaggtgacg
    14641 cggccggcgc gctcggtcag cagcttggcc ttgctcttgc caaagcccat ggcgccgccg
    14701 ccgccgccct tctgcacctg gcgcagggca aagaaggcca ccgccagaat cagcatgaaa
    14761 ggcagcgcct gcaccagcac gtaaaccagc acattgggct catccggcgc cttgccggca
    14821 tatttcacgc cggcctgctg cagcagctgg ggcaggctgg tgtcgttggg gatgggcacg
    14881 gtggagaagg catcgccgtt cttgtagtgg ccggtgatgc gctgctcgcc cacctgggca
    14941 tcggccacgc cgccttcggc caccttggcg cggaaatcgg aataggggat ctggctggag
    15001 cccatatccg cccgcgaacc gaacagcgag acggcaaaca gcaccgccag cacgatgccg
    15061 ccccacaaga gcaggctttt catccagggg gaaccattgg gctggtcttc gttcatcggg
    15121 gcggtccttt caaccggcac aatgtagggt ggccggggcg aatgacaagc cggggcaccc
    15181 gcgcacagat atcgccaaag ccgatttttc cgggcaaaaa gcccaggcgg ctgccctcac
    15241 ccgcgcgaga gcaggaacac cgcatgttcg gcgcgcagat tggccagcgc cgggccgatc
    15301 agcgcatcgc ccttcaaaaa ccaggcctgt tccaccacca gccccttggc ccttaccaga
    15361 tcgcggaaat cggcgatggt gaggtggtgg atgttgggcg tttcatacca ggccaccggc
    15421 agcagccgcg tcaccggcat gtggccgccc accagcaggc tcatgcgcac gcgccaatgg
    15481 gcgaaattgg ggaaggagac aaacgcccgc cggccaatgc gcagcaattc ggccagcacc
    15541 agatcgggcc gcttggtggt ctgcagcgtc tggctgagga tggcatagtc gaaactgtcg
    15601 tcgggataga gggcaaggtc ggtgtcggca tcgccctgga tcaccgacag gcctttttcc
    15661 acgcacagcc ccgcattatg cgcatccagc tccagcccgc gcgcatcgca ggcccgctcg
    15721 tcgcgcaggg ccgccatcag cgtgccatcg ccgcagccca catccagcac cctggccgcg
    15781 cgcgccacat gggcggcgat caccgccaga tcagggcgca gcgccacgga tacgcggttc
    15841 atcacgcccc cctcaccggc tgaggaaccc ggcgatcacc cggtcgagcg cgggcacgtc
    15901 gagcaggaaa ctgtcgtgcc cgaagggcgc ggccagctcc atgaaggaga ccggcgcccc
    15961 ggcggcattc agcgcatggg ccagcttgcg cgattcggcc gtggggtaaa gccagtcggt
    16021 gtcgaaggag acgaagcaga agcgcgccga ggtgccggca aaggcctccg ccagccggcc
    16081 ctgcccatgg gcgccggggc caaagcgttc ttccgccaga tcgaaatagc tcatcgcccg
    16141 ggtgatatag agataggaat tggcatcgaa acgcccggtg aagctgctgc cctgatagcg
    16201 cagatagctt tccacctgga aatcggcgcc aaagccaaag ctgatggcat cgcggttctg
    16261 cagccgccgg ccgaatttct ccgccagcgt cgcttccgac agataggtga tatgagcggc
    16321 catgcgcgcc actgccagcc cggcatcggg gctgcggccg gtgttgtagt aatcgccgcc
    16381 ctgccagtcg ggatcggcga tgatcgcctg gcggcccact tcctgaaagg cgatgttctg
    16441 cgccggcatc gaggcggtgc aggccagcgc cagcacccgc tcggggatat gcggccagtt
    16501 ggccgccagc gacagcgcct gcatgccccc catcgagccg cccaccacgg catagagccg
    16561 ctccaccccc agcgcttcca gcagcgccac atgggcgcgc gccatatcgc ggatggtgat
    16621 gacgggaaag cgcatgccat agggccggcc atcggggcca gggctggcag ggccggtgga
    16681 gcccatgcag ctgcccagca cattggggca gatgacgaag aagcggttgg tgtcgatcgg
    16741 cttgcccggg cccaccatgc gcgcccacca gcccggcttg ccggtgatgc ggtgggggct
    16801 ggccacatgc tgatcgccgg tggtggcatg gcagatgagg atggcattgc cgccatcggc
    16861 cgccagctcg ccataggttt cataggcgat gcgcacgccg gcaaggcttt gcccgccatc
    16921 cagcggcaag gggcgcgcca gatccagcgc ggcgctggtg gttaggaggg gggcgcttgc
    16981 catggttgcg cggaattggg ggcttgtggg cggcgtgtca atgggcgccc ctgttgtttg
    17041 tttcttcagc gcctgtttct tgccggggcg gccaaggcgg attaaggcgc catgccatga
    17101 cacagaaccc gcgcgcccaa gcccctgccc ccaagccctg gatcagccag atccacgcct
    17161 atgtgccggg caagtccaag gggcatgatg gccgcccgct gatcaagctg tcggccaatg
    17221 aaaacccgct gggcaccagc gccgtggcgc tggccgcgcg ggcagaggcc gccgcccccg
    17281 ccctctatcc cgatcccgac agcgtggccc tgcgcacggc catcgccacc ctgcacggcc
    17341 ttgccgcaga gcgcgtggtg atgggcaccg gttctgacga attgctgcac attgccgcgc
    17401 aagcctatgc cggccccggc gacgagatcc tctatgtccg ctatggcttc tcggtctatg
    17461 acattgccgc caggcgctgc ggggcggtgc cggtggtggc gcccgatgcc gactatggca
    17521 ccgatgtcga tgccctgctg gcgctggtga acgagaagac ccgcgtcgtc ttcgtggcca
    17581 accccaacaa ccccaccggc agcttcttgg cgcacggcga gatcgcccgg ctgcatgccg
    17641 gcctgccggc cgatgtgctg ctggtggtcg atcaggccta tggcgaatat gtggcgcccg
    17701 aggatgacga tggcgcgttg gcgctggccg aggcgcatca gaacgtgctc gtcacccgca
    17761 ccttctcgaa gatctatggc ctggccggcg agcgccttgg ctgggccacc ggcgcgcccg
    17821 cgctgatcga cacgctgaac cgcatccgcg ggcccttcaa cctttccaac accgcccagg
    17881 cgatggggct ggcggcgctg gccgatcagg cctttgtgga aagcagccgg cagcacaaca
    17941 cggcagaacg cgcccgcttt gccgaaaagt tggcggcgct gggcaaccat ggcatccgcc
    18001 cgctgcccag ccaggccaat ttcgtgctga tcctctttgc cggcgcgctg tcggccgagg
    18061 cggcttacga ggggctgatg gcggccggct atatcacccg ctggctgccc aatcagggcc
    18121 tgccgcaagc gctgcgcatc accatcggca ccgccgcgca gatggacgag atcgccgctg
    18181 ccctcaccgc catggccagc gggcaatgag cccccccacc tctgaccggg ccctctcgga
    18241 cgaagcgccc tttcgcaagg tcgccatcat cggccttggc ctgcagggcg gctcgatcgg
    18301 gctggcgctg gccgccgccc tgcccgccat cgccaccacc ggctacgacc tttccgccgc
    18361 caaccgccag cgcgcggccg aacgcgggct ggtgggccag gtgtgcgcca ccgcgccgga
    18421 agcggtgacc ggggctgatc tggtcatctt ctgcgtgccg ccgggcgcca tgggggcggc
    18481 cgcggccgaa gtggccggcg cgctggcgcc cgatgccatc gtctccgatg tcggcagcag
    18541 caaggtgctg atcggcgcgg cgctggccga agccctgccc gccgccgccg ctgccggccg
    18601 ggtgatcccc gcccaccctg tcgccggcac cgagaattcc gggcctgatg cgggctttgc
    18661 cacgctgttt cgccagcgct ggtgcatcat cacgccgccg ccccatgtcg atccggcggg
    18721 gctggcgcgg ctggtggcgc tgtgggaggc gatcggcgcg cgggtggagc tgatggagcc
    18781 ggcccaccac gaccgggtgc tggccgtgac cagccacctg ccgcatctca tcgcctacac
    18841 gatcgttggc accgccggcg atctggagga agtgacgcag agcgaagtga tcaaatattc
    18901 ggccggcggc tttcgcgatt tcacccgcat cgccgcctcg gacccgacca tgtggcgcga
    18961 cgtgttcctg tcgaaccgcg aggccgtgct ggaaatgctg cagcgcttca ccgaggatct
    19021 gaccgagctg caaaaggcca ttcgcctggg cgatggcgcc aagctgttcg atcatttcac
    19081 ccgcaccagg gccatccgcc gctcgatcat cgaacagggg caggatgatg cgcggcccga
    19141 tttcggccgc tccgatcacg caagatagca ccgggcgggg gccatccccg ccccgctcac
    19201 ccgttcagcg cgttgatcgc cgcctgcacc cttgcctcca cctcgtcacg cgggaggccc
    19261 ggcgggattt ccgcgccgaa gcgatagatc agcgtgcccg agcgcttgat ccagcggtga
    19321 tagagcggcc cgctgttcac cgccaccggc accaccggca ggccgatcag cttgtagagc
    19381 ccggcaaagc ccgcctgcag cggcgccggc ccgccatggg gcacccgcgt gccctcgggg
    19441 aagatcacaa ggtggcgccc ctcgcccaca gaggcgcggg cagcggccac catcgcgcgc
    19501 agggccctgg cgccctggtc gcgctccacc gggatcaacc catagcgcag ccccgccttg
    19561 ccccacagcg gcaggcgcaa cagctccacc ttggcaaaca ccaccggaaa gcggaacagc
    19621 accggtaggt cgatcgcctc gaagaagctt tcatgcttca tcgccaccag caccgggcgg
    19681 gtgggcatgg gcccttccac caccaccttc acccccatga tccagcgcat gcaccagcgc
    19741 tgcccggccg cccaccagct gcaccagcgc aaggtggccc cgcgcggcag caccagcacc
    19801 agcggaatga acagcgccat ccccaccgtg atgccataga aggcgggata gaacagcagg
    19861 ctgcgcagca ggtctgaaag acgcgccgcc tcccccgtcg ggcttgggtt catgtcaggc
    19921 ttaccccctg tgcgccaggc gaagcagccg ccgggcgaga tatttcgaat attccaccat
    19981 cagcatgttg aggctggggt gggtgggcac ggcatcttcc accaccgcca catcggccgg
    20041 gatggtgcgc gacagttccc aggcggcgcg gcgcatgtgc cagtcggaag tgaccaggcg
    20101 cagcgaacgg gcgtgatggg cggcgatcca gttggccgcc tcgcgcgcgt tgctgcgggt
    20161 gtccaccgat tcgtagccca gcgtgatgca gcaggccatg gtggcggcac tcacctgata
    20221 gcgggcggca aattcgcccg ccgtcacctg cgaatccacg cccgagacca gcagccgctt
    20281 ggcccagccc cgccgcagca catccagcgc gcgcggaatg cgcccctcgc cgccggtcag
    20341 cacgatcacc gcatcggtgg gctggccccc ggccgggcgc ggcagggtga cggcaaaggc
    20401 cagaaagccc agcaccgctg ccaccagcaa cagggcaaga agccgccgca gcatggccta
    20461 gagcatcttc ttcagcgcgg cgagcacggt gagccgcgcg gtggccatcg ccagcagcac
    20521 gcccgccacc ggcacgcccg ccagcgccag ccaatccggc caggccagcg cgccatcgct
    20581 caccagcccc gcgcccagca cgccaaaacg cgtgcccacc agccacagcg cgcccgcccc
    20641 gcccgccagc ccgacagcgc cccccagcgc cgcatccagc gcgatggagc gctggaacac
    20701 gcgggcgatc tgccggtcgg ttccgcccag cagatgcacg atctccagcg tggcgcggtg
    20761 gttgcccagc cccgtgcgca ccgccagcag taccgccgcg cacagcgccg cacccagcag
    20821 caccaccagc ccggcggcca gccactgcag gctggcgatg gcgccgaaaa cgggggccag
    20881 ccagctcgcc tgcgtatcca cttccagctg cggggcgtgg ccggcagccg ccgcgcgcaa
    20941 ggccgccagc cggctggccg aggccgggcc atccagcgtc acatcgatca gcgcggggct
    21001 gggcaacagc gccggatcat tctcgccgcc cagccagggg gcaatcagcg catcaagctc
    21061 ggcctcgggc acctggcgcg cggccagcac gccgggctct gccttcagcg cggccagcac
    21121 ggcagcggcc tgctgctggc gcagatcggc ccgcggctcg aggatctgca cgctgacgcc
    21181 atggcccagc gcgccgccgg ccgccaccgc gatatggcgc agcgccagcc cgctgcccgc
    21241 cgccagcacg gtgagcccca ccatgatggc gatcatccac ggcatcgggc cggaaaggcg
    21301 ccccggtggc agcatgtcgc gcccgctgcg gcgccagcgg ccgggcggcg gtgcggcagg
    21361 gccgggcgcg ccggccgctt ccctggcggg cgaaaagccg ttcatggcgc ccccacgccc
    21421 ggccggcgcg gcggatagcg caaggcgccg gtgggatcgg ccagccgccc ccggtccagc
    21481 cgcatgatca ggctgtcctg cacctgatgg agcaggtgca catcatgggt tgccaccacc
    21541 accgtggtgc ccagccggtt cagcgcctcg aacaggcgca gcagtttcac cgccatctcc
    21601 ggatcgacat tgcccgtcgg ctcgtcggcc accagcagtt cgggccggcc gatcaccgcg
    21661 cgggcaatcg ccacgcgctg ctgctcgccg cccgaaagcg tggccggcag ggcctgcatg
    21721 cgccctgcca ggcccaccca ttccagcata tcggccaccg ggcgcgccac ttcgctttcg
    21781 ggcacgcccg ccacccgcag cggcagcgcc acattgtcga aggccgacag atgcggcacc
    21841 agccggcaat cctgaaacac cacgccaatg cgccggcgca ggcccggcag ccgttcgcgc
    21901 ggcagggtca caccatcgat gccgaacagg cgcaccagcc cgcgcgtggg ccgctgcgac
    21961 agatagagca gtttcagcag cgaggtcttg cccgcgccgc tggcaccggt gaggaaataa
    22021 aagctgccgg cataaaggct aaagcccaga tcgctcagca cttccgggtc cgacgagcca
    22081 tagcgcaggc cgacattgtc gaaatggacg atctcgcccg tctggggcgg cgcgcttacc
    22141 atgcgcggcc tccctgtgaa tatccggcct gtgaatatcc ggcctgtgaa tatccggatc
    22201 gttgtgcgcc agtgcctgcc atgcccgtgg ctatagcggc caatccatgt ggaaaaaaga
    22261 gcattggcgg ccggcttttt ccgcaggtgc cttgtatggc cggccactgc catgcattag
    22321 aatggtgcca tgatcatcgc ctgccccgcc tgcgcgaccc gctatgccct gccggatgaa
    22381 gccatcggcg cggccgggcg cacggtgcgc tgcgcgaaat gccggcacag ctggttccag
    22441 agcgggccgg aacagcccgc cccgcccccg cccgcagcgc ccgtggcaga accggcggcg
    22501 cccccgcctt ccgccccgcc ccggcccgca gcggcagtgg ccgaggcagc ccgcatgccc
    22561 tcgcccgagc agctgcgcga ggcgctggcc cgcaccatgg ccgccgccac cgccagccag
    22621 gccatccccg cctcgatcct gcccacagcg cccgccccgg cagaggtggc cgaaagcgcc
    22681 cctgcggccg agacgcctgc cgacgagccc tcctatttcg agcatgagcc gcccttccgc
    22741 ccccgccgca acccggcccg gctgtggacg gcagcggcgc tgatctttgc cctgctgtct
    22801 tgcgcggcga tcgcggcggt ggcgcgcttt ggcctgcccg actgggtgcc gctggccacc
    22861 agcggctttg gccccgccca gccagacctg gccctctcct tcccgcccaa ccggcaagac
    22921 cggcgcaccc tgcccgatgg cacgcaattc ttcggcatca ccggcacggt caccaatatc
    22981 ggccagcagc gccgcgcagt gcccaccctg ctgatcctgc tgcgcgacga caagaatcgg
    23041 gtggtctaca gctgggaagt gaccgcgccc aagcgggtgc tggcgccggg cgaaagcgtg
    23101 ccgatcatcg aggccgccac cgacattccg caaaccgcca aattcgccga aatcggctgg
    23161 aaaccggcct gacgcgcccg cagcgccagg cctttgcatt tttacccggc attgggcaat
    23221 tgagcgcttg cagagggcag ggtgctttgc taatggcccg ctcctacccg gcggggcggt
    23281 gcttttgggc ccatccttgc tgcctttgga tgcggtcgtg gcggaattgg tagacgcgca
    23341 acgttgaggt cgttgtgggc gaaagcccgt ggaagttcga gtcttctcga ccgcaccaaa
    23401 atttcttgca gaacaggctg gatcctcccc tttcccgggg ccgcgccctg ctgcgcccca
    23461 cacagcaaaa cagcagcacc gccgtttccg gcgcagcgct gctgcctgat gccctgatac
    23521 ctgcttgggc cttgcagcct cagcccgaca ggctgagatc ggcaaagctg ccgaatgtgc
    23581 ccgccgccgt atcgggcagg ggcgaaagct ggcggctata gaccagccgg ccgccgcccg
    23641 acgaggtgta gagcgggatg cgcggcagat cggtcatcac cagctgtgag aaagcggcat
    23701 agagcgcgcg gcgccgcgca gggtccgtct ccacctgggc ggtttccagc agatggtctg
    23761 ccgccgggct gttgtaatgg ctgccattgc agaaagcgac cccgcgcttg aagcttttcg
    23821 accaatacca gcgctgcatg ccgatcaccg gatcggggcc catctgcccg ctgttgacca
    23881 gcacttccca atcccgatcc gtgtagacgc gccgcacgaa agtggcaaaa tccaggcttt
    23941 ccacctgcac cgcaatgccc acgcgggcca gtgcgctgcg gatataatcg gccaccttgg
    24001 cggtatcgtt gttgccgccg gccgaattgg gcgagaggcg aaagcgcagt cgcacgccgc
    24061 tggcatcggg cttcagcccg gcggcatcca gcagggcgat ggccttcgcc ggatcatagg
    24121 gatattcggg cacatcgtct gtgtagaaat ccttgtagat ctgcgagatc ggccccttgt
    24181 cgatcacgcc gccgccctgc aggatggtgt ggaggatgaa ggtcttgtcg atcgcatggg
    24241 cgactgcctg gcgcacccgc acatcctgca gcttgggcac atcgaggttg aactcgatcc
    24301 ccgtgcccga tgtggcgagc gggaagaggc ggtcatcggc cctgaggttg ggcagtttgg
    24361 ccaggcgctg gtaatcgcca ttgggcacgc cgggggtgaa ctggatctcg ccggtttcca
    24421 gcgcggtcga cagggccgcg ctatcgccca ggatgcgcag gatgatgcca tccacatgcg
    24481 gcttgccctt gtcccagtaa tcggggttgc gctccagccg caggaactgc ccgcgctgcc
    24541 attcggcaaa gcggaaaggg ccattgccca ccggcgcgat gttggccgga ttgctcagca
    24601 catcgcggcc ttcatagagg tggcgcgggg ccacttgcga ttcattggcc gccagcgcgc
    24661 tgatcacata gggcgagggc ttgtcgaagc gcaggatggc ggtcagcgga tcgggcgtgt
    24721 ccaccccggc gaggtgggca taggtcatcc gcccgcggct gtggaactgc ttccacaggt
    24781 gcagcgccga gaaggccacg tcatgcgagg tgaaatcgcg gccatcgtgc cagcgcacgc
    24841 ccgggcgcag cttcagcgtg agggcgcggc tgtcggcgct gaaatcccag gcctgggcca
    24901 ggcccggccg cggcttcaga tcgaaggtgt agttcagcag cccgtcggcg atcttgggcg
    24961 acagccattg cgcctcgccg gccgtggtga tcgccgccac cagaaaggcc ggatcattga
    25021 cgatcccgta gatcagcgtg ttgcccgccc ccgccttgcc ggcgcaggct tccagcagcg
    25081 gcaggccggc cagcgccgcc gcgccgccca aaaggccgcg gcggctcagc gcaaatcctg
    25141 tgttttcagg catgatatgc tcccgatcgt aaaagggcgg cggtcaaagc gccagcgggg
    25201 cggcggcatt gcgcgcctct tgcgcgtccg cctcttgccc cccgccctgc ggcgcctggc
    25261 caaaagccac cggcaaggtg ccgttgacgg caaaggcgcc cacgatgcga tcgcgataga
    25321 tgcgcggatt gtgcgaggtg atcgtgcggg cattgcgcca gtgccggtcc agccccagcg
    25381 cgcggccggt gcccgatgcg cccagcgcgt cgaagatggt ggtggccgca tccagcacca
    25441 gctcgctcac cacgctcacc gcctcgttga tttccagatc ggcgatggcg ccggccgctt
    25501 cctcggccgc gggcaagccc gcgcggttgg cctcatacac gcgctccagc gcttctgcca
    25561 ctttcagcgt gatggcgccg gccgcatagg cgcccccgcg cgcccggccc accaccgcca
    25621 gcacctgcgg atcctgccgc gcttcggccg cattgccatg gccataggtg cggctgcggg
    25681 cggccacggc ctgcgcaccc tcatcggcca ccgcccgacc aatgccggcc agcgtggcaa
    25741 tatggatcag ctggaacagg ccgcccacat agcgggcgcg cttcgtctcg ggcttcacca
    25801 gatcgtcggt cagatagaca tcgcggaaca gggtggagcc gctggcggtc agcgtctggc
    25861 caaagccgtt ccagtcgtcg ggcacttcca cgccgggggc ccggcggggc accacggcgc
    25921 ccttgggcgt gccgtgctcg tccaccgcgc ccatgttgat ccagtcggca aacagagtgc
    25981 cggtggtgta gtatttctgg ccattcagcc gccagccctc gccctcgcgc accagccgcg
    26041 cgctggtggt gccaacattg ccggccagtt cggaaaagcc gttgccaaag gtcttgccgg
    26101 cggcaatctc gtccagccag cggcgggcat aatcggcatg ggcggtgttc agcagatcct
    26161 cggtgaagcc gatatgcggg cgcaggatat tggtgatgtt gggatcggct gctgacagtt
    26221 cgatcagcag attatgcagt tccggcaggc tgacccccag cccgcccagc gccaccggca
    26281 ggcgcagcct ggtaaagccg gccttcttca gccattccag ctcgtcgaac ggcaggcggc
    26341 gctcggcatc gcgcaccacc gccccttcgg caatccgccg gaagaggggg cggaacggcg
    26401 cggccagcgc ttcataacgc gcgctgggcc cctgccccca gccttgcgcg atctggccgg
    26461 atgacggggc gacggcatcg gccagaggct caggccgcgc ggcgatggca agctgggtca
    26521 tgggtgcact cctgttttgc gggcggcggc cgggcgccgc ccgcgcttgt cctcaaaaat
    26581 cgatggaaac gccgccctcg gcctggaacg gcgcctgcgc attggccacg cgatagacag
    26641 cgccccgctg cgtgtaggtg ttgttgaaga agccggtgga tgtggtcgcc atttcggcaa
    26701 agcgatcgcc ggaatagacc acgcccgaga accacacttt caggttgcgc agatgcggcc
    26761 gttcatactg gatcttgaag gccaaccggt cgagctcgcc attcttgagc acgcctgccc
    26821 catagacatt gcccgccgag gaagaggaga gcaccaccgt gctgtagggc gcgacattac
    26881 tggtgagata gctgccgccg gtcaggctgt attgcacggt gaggcgcagc ttctgctcgt
    26941 cgcggcccac aggggtggcc gcatcgatgt tccagccggc cgtccacggc ggcgtgccgg
    27001 tgacaaaact gccattgctg cgcagatggg catccacccc gttgaaattg ccccccaccc
    27061 tgaggctggt gccctcgcgg tcgtaaaggc ggatgcgcgc atcca
//
LOCUS       DASRYE010000069        20707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_37600,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000069 DASRYE010000000
VERSION     DASRYE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37600"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1378)
                     /gene="phaZ"
                     /locus_tag="VFF98_03905"
     CDS             complement(158..1378)
                     /gene="phaZ"
                     /locus_tag="VFF98_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZU62806.1"
                     /translation="MLYQGYQAFTDTLAPARWLAGEALRWRDAHGGALARLDWPRRLF
                     ALADVFVQARITHEAPPFGISMVHCGNSAQPVVEEVAVDAPFGRLIHFAKPEITTPQP
                     RMLVVAPLSGHYATLLRHTVEVLLRDHDVYITDWKNARDVPASAGRFGLSEYIDYVIA
                     FLRHLGEGAHLLAVCQPCVPALAAVAIMAQDNDPCQPHTMTLMGGPIDVRAAPTVVNE
                     LAMSHSIDWFADNLISSVPWRYQGAGRRVYPGFLQLSAFMAMNLGRHFGQFRKLYQHL
                     AQGEDADARKISDFYEEYFAVLDLTAEFYLETVEEIFQKAALATGELLHHGRRVDPSA
                     IRRTALLTVEGERDDICAVGQTAAAHLLCSSLRPHLRRHHLQPGVGHYGVFAGSKWEN
                     QVYPQVRNLVLAMS"
     gene            1544..2197
                     /locus_tag="VFF98_03910"
     CDS             1544..2197
                     /locus_tag="VFF98_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZU62807.1"
                     /translation="MTKATLTISSKTYSAWSLRGWLLCRLAGLEVDEQPVTHDANDRA
                     ELLLLSPSVLVPRLTHEGASVWDTLAIAEYLNELYPEAQMLPADRLARAHCRSVSGEI
                     HSGFANLRSALPMNLKVMHKRFPIFSGAKPDIERIETIWQECLARYGGPFLFGKAPTI
                     ADAMYAPVTRRFLSYAVALSAPSAAYCETIANWAPMQEWVAAALKEPEEMVDLDIEF"
     gene            complement(2237..2968)
                     /locus_tag="VFF98_03915"
     CDS             complement(2237..2968)
                     /locus_tag="VFF98_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HZU62808.1"
                     /translation="MEKPIRLNISRRHVLGQGLGLGLCALGTGVVGLALPKSAPAQSF
                     GASDAAPADPPLSGEPITVPAGAHDLTAYERRIVDVARRELERQQALLWRQDIVAVAD
                     FARPSALPRIHFVDMEAGRLRSYLVAHGRGSDPDHSGWLERFSNTVGSEATSRGAYLT
                     CACYEGKYGPSIRLRGLDADNSNALDRAIVMHPAWYAASEMLPRYGKLGRSEGCFAMD
                     PADFSVALNQLAAGRLLFADRIGEG"
     gene            complement(3012..3509)
                     /locus_tag="VFF98_03920"
     CDS             complement(3012..3509)
                     /locus_tag="VFF98_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU62809.1"
                     /translation="MTLAIRPAAPADLPLICQFIRDLAAYERLSHELRFSEAELGRHL
                     FGPRPMAEALIGEIHGLPQGFALFFHSFSTFEGRPGLYLEDLFVRPEARGAGLGKALL
                     LQLAALATARGCARLEWAVLDWNAPSIGFYRALGARPMEEWTVMRVDGDALQRLAAEA
                     GTSAE"
     gene            complement(3550..4113)
                     /locus_tag="VFF98_03925"
     CDS             complement(3550..4113)
                     /locus_tag="VFF98_03925"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZU62810.1"
                     /translation="MLILGLMALAQALMHARPPASAPGASTSLAPNPFADATPDVFHQ
                     SAGAWGNTPPPPPPGSPAQPSGPVTLMRDGRGQFHLTAQVNGQDTDFLVDTGADVVAL
                     TEAQANRLGLNVAPADFRPVLRTASGTAMGAPVHLDRLVLAGRSFADVDAVVIQGAAI
                     NLLGQSALRRLARVELRGDTMVLDPGS"
     gene            complement(4208..5821)
                     /locus_tag="VFF98_03930"
     CDS             complement(4208..5821)
                     /locus_tag="VFF98_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU62811.1"
                     /translation="MAYDYIIVGAGSAGCVLAERLSADGKARVLLLEAGGANRDTMVR
                     MPRGMAKIWMNPRYYWPFPVKQAGQRPAGETWYYGKGLGGSSAVNGTWYYRGQPRDYD
                     AWEAQGNAGWNWAEIERCYAAIEDYHGPAPAAGRGKGGPLEVITSTDDSPLTRALLRA
                     GEQFGLPVLGDVNQPGQAGIGLSQMTVDRKGNRVTAFTAFLGKARHRPNLTIRTGALV
                     QRVLIEQGRATGVLLASGEEIRAGEVILAAGVLTSPKLLQLSGIGPAELLARHNIPVV
                     VNNPAVGANMSEHMMVSLSFRLKGMAGINREFRGWRLYANALRYFLTGTGLMARLVPD
                     VSAMISTTGDASWPDLQLGIAPYSMESSADDKPEAGRGVPEKVPGITVAAFYLRPFGR
                     GRVEIQSADAASPPQVDAGWLADPRDGAAAQRMIRAIRGFVRQEALGAYIGEETVPGP
                     AVSSEEEITRALWWMLSTGLHGTGTCRMGPASGAEGGDAVVDAQLRVHGVSGLRVVDC
                     SIMPTSISGNTNGPAMAVAWRAAELIGQH"
     gene            complement(5834..7150)
                     /locus_tag="VFF98_03935"
     CDS             complement(5834..7150)
                     /locus_tag="VFF98_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZU62812.1"
                     /translation="MGAGISLKERVSYGLGNFGVNLIFGATGAYLMYFYTDVYGVSPA
                     TVASLFLLARGVDALFDPLLGLAIDRTHTRWGKHRPYLLWFALPFAASGVAVFAAPAL
                     SGLAKLAYIYVSYTAVGLLYSCISLPLNSMLPTLTRDVRARNTTNAIRELLGSSATVG
                     VSYAMLPLVGALGGGDQQAGFVRAALLLAVLTLAGVAIAFANTREREAPEASPERLTT
                     RQSLLATRGNWPWIATMLVNLAFWIGFIGHIQSTIYYARDVAGHAEWAPPLMAMMLVI
                     LIGTALSALVANRIGKPATGMIGAGIGAICTAALPLGTSLAWLMAMNALAYAGLGLLG
                     GLLFAFMADAVDFGAWKSGFRAQGFLFAASSFGVKLGMSIGGAAGAWLLARAHYAPGL
                     PASPQVISAITWSHAGLPALSYLAMAAALLLFRFPPDYPRGTEKRA"
     gene            complement(7164..8132)
                     /locus_tag="VFF98_03940"
     CDS             complement(7164..8132)
                     /locus_tag="VFF98_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU62813.1"
                     /translation="MPASPSSATTTQAYAIDLITFFHPSFWGLANEDEVVALGKAEPR
                     PFWDRMLDAVAQSGARGIELTFSPFGWRDTLAAYGSAAGFAEALAARGLQLASGFFAD
                     VAIGGDLETAETRAAYIAEASLYAEYLGSFGVDAMVMGLPMRASWDSQPPQIASFDTA
                     KTIADFCNLLGAATLRHGVQLALHTEAHSLFSLARDIDLILLLTDPVFVGFCPDTAHI
                     LLGGANPVEVVARHKERLIATHWKDATGPAPVRIPIDHQVHLSHRPYFCALGAGKVDW
                     AGWQALLTDARFAGWNIVEIDAVPDPVAEVRRSLAFLGALPVPANQ"
     gene            8541..9656
                     /locus_tag="VFF98_03945"
     CDS             8541..9656
                     /locus_tag="VFF98_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU62814.1"
                     /translation="MALQTFALIGGGPGSFIGPVHRIAAELDGEMRLVAGAFSSSAEK
                     SAAAGAAYGVDPARAYASIADLVAGERARPDPVDFIAIATPNHMHLPAARAALEAGFP
                     VICDKPATATFAQAQELAQIVARAGLPFGLTYTYSGYPLVREARARIAAGALGEIRKV
                     VVEYPQGWLAKPSEGKQAAWRADPSRSGPGGCVADIGVHAFHLAEFISGLQVSEILAD
                     LGTVVPGRQVDDDCQILLRFANGARGALLASQIAVGERNGLAIRLYGEKAGLAWRQEE
                     PNRLWLYHEDGRSELIQAGDPGLGADALRASRTPGGHPEGYLEAFANIYRDFGRLLRG
                     EAGALIPGMADGLRGMALIEQAVALSRAGAGWTAFTV"
     gene            9671..10726
                     /locus_tag="VFF98_03950"
     CDS             9671..10726
                     /locus_tag="VFF98_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU62815.1"
                     /translation="MRNLKGPGLFLAQFAGDNAPFNSLSAIADWAAGLGYKGLQIPSW
                     DARLFDLARAAESQTYCDEITGMLAEKGLAITELSTHLQGQLVAVHPAYDAQFDGFAV
                     PEVRGNPAARQAWATEQVKLAAVASRRLGLAAHASFPGALAWPYVYPWPQRPAGLVED
                     AFDELARRWRPILDVFDENGVDIGFELHPGEDLFDGVTFEMFLERVENHPRANILYDP
                     SHFVLQHLDYLAFIDIYHERIKCFHVKDAELRPSGRSGVYGGYQSWVDRPGRFRSLGD
                     GQVDFAQIFSKMAQYDYAGWAVLEWECALKHPEAGAAEGAPFIARHIIRVTEHAFDDF
                     AAGGADRALNKRIMGID"
     gene            10815..11507
                     /locus_tag="VFF98_03955"
     CDS             10815..11507
                     /locus_tag="VFF98_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU62816.1"
                     /translation="MTFAGLGLMGGLALGLASSLHCLGMCGGIVSSLMLAGGGRRAPG
                     WAGRLARLQGGRVLAYSLLGALCGGLGGAVFGHLAGPVLFLVLRSVAALSLGWVGLTL
                     TGLAPRVPLAERGLALGAAGLRRVQALVPAVLAPVLTGMIWGLMPCGMVYGALAFALV
                     SGSALGGGLVMAGFGLGTLLPVSAAGAGVAWLGQRATGPRMQAALGVVLMGGAAASLW
                     VSPEAVRALCGG"
     gene            11704..12690
                     /locus_tag="VFF98_03960"
     CDS             11704..12690
                     /locus_tag="VFF98_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU62817.1"
                     /translation="MQGRRVVSGPVAGCVQASLRHHTDDVGKCDSRYALSERVDICIC
                     TFRRSDLYKTLESICHQIDTDNLQLKVIIIDNDDVESARPVVDRAIARLKIDCLYLHA
                     PARNISLARNAAFAATSAPYVLCIDDDEVAAPRWVRHMVDALRQSAAGVVIGPVHAVY
                     SPHHPRWMREMEPHSAIPRGNYRAARLTHIGNAGFRGAVIRGRRFDLLLGRSGGEDTE
                     FFSRLRREAVPITYCDEAKVFEPVSEERASMKWLVERHFRIGQTYARSERPAGGGRLP
                     LAARAAAKALACLGLAAANLGHEGRAKCHFLRFVMHAGVLMNALGLPPIELY"
     gene            12703..13440
                     /locus_tag="VFF98_03965"
     CDS             12703..13440
                     /locus_tag="VFF98_03965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU62818.1"
                     /translation="MSLSLAQKLSFRLATGWQQRTMARAVAGPVLSIGFDDAPRSAWT
                     MGGQVLADHGVRATYYVCGGLVDTDGELGTYLTRADLRALVAAGHELGCHTFDHSSAL
                     LAGAGRFAASLEENARWLADFTGGVAPCSFAYPYCHVGLAARRAAGARFHTARAGGRA
                     WNGPVFDPAVLAGWPLEAREGAVDYADLCQAAARAGGWLVLFSHDVSERPGPYGCTPR
                     QLDEVLRAARRAGMAILPAGDVALVRR"
     gene            13902..19772
                     /locus_tag="VFF98_03970"
     CDS             13902..19772
                     /locus_tag="VFF98_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62819.1"
                     /translation="MSTSSANPTVINGSSSSSVISVSGTNVTVGSSTTNTLFTDFHTV
                     GGSGSGGGAGLGGVFYVDSGESLTLVNVSFSSNLAQGGTGGTSTTGGSMNGLVTTSSG
                     TSGAAGSDAASDYSSYNSTNGGPGYAGHTGSAASSGVGGNGGKGGNGSSGSTSSDDII
                     LSALTAAYDLFKAGTTSDTAETYSTFAEEFSALAIEASSGANAGGPTTETLVAGLEAI
                     AATFTKLSTETASQASQDYEQEAAQEAYQIKLTMDAYTAGASGNGGAGGAGGNAGAGS
                     TFFGGGDGGNGGNGGNAVSSTGTTGGGIGGDGGDGGTGGNGGFGAGGGSGGTGGTGGT
                     SASSDNVNGADGSDGAGGTGGFGAGDGSTGSEKNSDGTYGSGGGGGSGYGGAIFVRSG
                     GSLTVTGNSTFYDNATEGGSSENGGAAGDSAGSDIFMMKGSTVTLAPGVGNTITFYGS
                     IADDSAASIDDASYAAGNGASLIVTGGGTVKFLGTNTYTGTTYIDGATLEADNGVGID
                     TLSHVAFNGTGSINNSLSTLDAGVWLTSGTLTRRVGTLDTQISWGGSGGFAATSAGLT
                     LNFGSINGGLGQTLTWGSGSFVPVGSTLIFGSDATDATGVVTLLNNINLNGNQGQIAV
                     YNNASGTAYAVISGNISNGTLLVGDTDYSGTLYLTGTNTLSGLTVQNGTVSTLLGTTV
                     GQLMDPTNGGYLTITGGQVILGGAEKLTSVNLASGTQLTAYGALTTNDITNVGTILDK
                     STLNAGTITNTGSLTVDGAVTSSWINNTGTIDFAVNATTGAITNSSTGAITVGGNLVS
                     GAIGNDGSVIVDGTANTGAVTNTGTFGVKGNATTGALSNSGTMVFDANLTSGAITNSG
                     TFSVTGNASTGAIDNTATGNGVIGGTLTTNGATLINDGTFSVGGNAATGNVTNSGTLT
                     FASTAQIGTLTNSGTAYLDGTTTSGAISNTNLLVQNGAVTVSGTVSNSQGASWVLAAD
                     LTSTSTVTNDGTLWVVGDNDDSTAATRTITTTGFGGASTGYVGLGGLDGTLANTLVID
                     QSGNSTYAGVFSGAGSLVKEGAGTLTLTGASDFTGGLTVAAGTIDTTGGGTFADTLAI
                     TVDKGAELIMGTADLVGSITNAGTLLATANLGLTTLTNSGTATIDAVLAASGNVSNTA
                     DGTLTLASTANAQIAGTLTNAGTLTNNGTLYVDGAVSNSGTLTLASGSTTELASLTNT
                     GTITAYAPLTVVGSYTQNAGTLTAYDGLYTGTLSGSGGTINLAGSTYTLNQTANGTYS
                     GSIVGGGTVDKYGTGTLTLNGAADSFAASQLNIYAGDVTVATANLLDSALNVLIDTAG
                     TLTLEANQTIHDLTGAGTLDIGTSTLTLSDGGEFSGTIDGTGTIDVTDGNFTLNSAIN
                     NTGGTFTVGTDSTVTVGASGSLTTTNLNVTGGTLDLIGKATATTTTLTDGATLHLGNG
                     VDLDESGAETGTLTSTTTYVLGGSAITGNGSISGTVYVGGASAGIVAPGNSPGILSFD
                     NITFGNNSTAEMQVDGTAGAGVTGGNDLIEVSGQLTLTGSSTLAISKSVSNSFALSLG
                     EQIQLFKFSAGKVKGYFGSVTLSDYSGSALYNVATGTLIGLGSYTPASFKAAVSTTAN
                     QAAIMKALMVNDAGGVPQYYGGNLMSYVTSALASNPASVAGVFARWSPEAYAGIMDQM
                     KFSMLDNLPELGGYDKLTPGRTYATGSFNRGGLKGEGQTGYAQNNLRDDGYNVGIAHQ
                     FGFGQLTLSYGHSDGNFYGTYIASRTRGNQVGLGISAPVALGQALRVTGRLAYGDYNS
                     HGTRSTNSGTADFAGVKSNIFVYGAGLEYLKQAGQIRVDTTAEFLGMHERMSGFTEYG
                     AADTLDQMSVERSTHEAYLARLGATVGYTVSPLVEVYAKGTYDHEFGHQMTDITAHVA
                     VEDTAFTVANTGLARDRVSAGGGVKFNLTSRLQLNLDASAGTNASYRYNAAVRFGF"
     gene            19911..20174
                     /locus_tag="VFF98_03975"
     CDS             19911..20174
                     /locus_tag="VFF98_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62820.1"
                     /translation="MPDRMARIALALLAGALAVPAAHADPQPTTEAAAPRDAHTGLAT
                     GKRQHEPVSIHKTVDAATPKAMASPSTPADTAKVKAHSNTNNN"
     gene            complement(20279..20352)
                     /locus_tag="VFF98_03980"
     tRNA            complement(20279..20352)
                     /locus_tag="VFF98_03980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Gln,seq:ctg)
BASE COUNT         3537 a         6791 c         7108 g         3271 t
ORIGIN      
        1 cgctcgacga tggcctgcgc ctgctggaag agcaccgcgt gccggcggtg ctggtggtgg
       61 cgcagaacgg ccggctggcc ggcctgctca cgctcgacag cctggggcgg atggcgctgc
      121 tgggccggct ttcccgctga agaggtcgcg ctgaacttca gctcatcgcc agcaccaggt
      181 tgcgcacctg cggatagacc tggttttccc atttggagcc ggcaaacacg ccataatgac
      241 ccacgcccgg ttgcaggtgg tggcgccgca gatggggccg caggctggaa cacagcagat
      301 gcgccgccgc cgtttgcccc accgcgcaga tatcgtcgcg ctcgccttcc acggtgagga
      361 gggcggtgcg gcggatggcg gagggatcga cccggcggcc atggtgcagc agctcgccgg
      421 tggccagcgc cgccttctgg aagatttcct ccaccgtctc gaggtagaat tccgccgtca
      481 ggtcgagcac ggcgaaatat tcctcgtaga aatccgagat cttgcgggca tcggcgtcct
      541 cgccctgggc caggtgctgg tagagcttgc ggaactggcc gaaatggcgg ccaaggttca
      601 tcgccatgaa ggcgctgagc tggaggaaac cgggatagac ccggcgcccc gccccctgat
      661 agcgccaggg cacgctggag atgagattgt cggcgaacca gtcgatcgaa tgggacatcg
      721 ccagctcgtt caccaccgtg ggggcggcgc gcacgtcgat cgggccgccc atcagcgtca
      781 tcgtgtgggg ctggcagggg tcgttgtcct gcgccatgat ggccacggcc gccagcgccg
      841 gaacgcaagg ctggcacacc gccagcagat gcgccccttc gcccagatgg cgcaggaaag
      901 cgatcacata gtcgatatat tccgacaggc cgaagcggcc ggcgctggcc ggcacatcgc
      961 gcgcgttctt ccagtcggtg atgtagacgt catggtcgcg cagcagcact tccaccgtgt
     1021 ggcgcagcag cgtggcataa tggcccgaca gcggcgccac caccagcatg cgcggctggg
     1081 gcgtggtgat ctcgggcttg gcaaagtgga tcagccggcc aaagggggca tccaccgcca
     1141 cttcctccac caccggctgg gcgctgttgc cgcaatgcac catgctgatg ccgaagggcg
     1201 gcgcctcatg ggtgatgcgc gcctgcacga acacatcggc cagggcaaac agccggcgcg
     1261 gccaatccag ccgggccagc gcgccgccat gggcatcgcg ccagcgcaag gcctcgcccg
     1321 ccagccaccg cgccggggcc agagtgtcgg tgaaagcctg atacccttga tagagcatgc
     1381 aaattcccca aagcggcatg gcttgcctgc catgcgccgc aggcccgtat gccgcgcaat
     1441 tgcccgcgct gcacccgcgc cattttacag gttcatgttg cgctgcatca tggcatattg
     1501 caacctgaat gctgtagcga acggaacggc cggagagcat gcgatgacca aggcgacgct
     1561 gacgatctcg agcaagacct attcggcctg gtccttgcgg ggctggctgt tgtgccggct
     1621 ggcagggctg gaggtggacg agcagccggt gacgcatgac gccaacgacc gggccgaact
     1681 gctgctgctg tcgccctcgg tgctggtgcc gcgcctcacc catgagggcg ccagcgtgtg
     1741 ggacacgctg gccatcgccg aatatctgaa cgagctgtat cccgaggcgc agatgctgcc
     1801 ggccgaccgg ctggcgcgcg cccattgccg ctctgtttcg ggcgagatcc attccggctt
     1861 tgccaacctg cgctcggccc tgccgatgaa cctcaaggtg atgcacaagc ggttcccgat
     1921 cttctcgggc gccaagccgg atatcgagcg gatcgagacg atctggcagg aatgcctggc
     1981 gcgttatggc gggccgttcc tgtttggcaa ggcgcccacc attgccgatg cgatgtatgc
     2041 gccggtcacg cggcgcttcc tgtcctatgc ggtggcgctg tcggcgccta gcgccgccta
     2101 ttgcgagacg atcgccaact gggcgccgat gcaggaatgg gtggccgccg cgctgaagga
     2161 gccggaggag atggtggacc tcgatatcga gttctgaagg ggcggccttt cagccccctc
     2221 cgtcagcccc cctccgtcag ccttcgccga tgcggtcggc gaagagcagg cggccggcgg
     2281 ccagctggtt cagcgccacc gagaaatcgg ccggatccat cgcgaaacag ccttccgagc
     2341 ggcccagctt gccatagcgc ggcagcatct cgctggcggc ataccacgcc ggatgcatga
     2401 cgatggcgcg atccagcgca ttggaattat cggcatccag cccgcgcagg cggatcgagg
     2461 ggccatattt gccctcgtag caggcgcagg tgagataggc cccgcgcgag gtggcttccg
     2521 agcccaccgt gttcgagaaa cgctccagcc agccgctgtg atcgggatca gacccccggc
     2581 catgggcaac cagataggaa cgcagccggc ccgcttccat atcgacaaaa tggatccgcg
     2641 gcagggcgga agggcgggca aaatcggcca cggccacgat atcctggcgc cacagcagcg
     2701 cctgctggcg ttccagctcg cgccgggcca catcgacgat gcgccgttca taggcggtga
     2761 gatcatgggc gccggccggc acggtgatcg gctcgcccga aagcggcgga tcggcaggcg
     2821 cggcatcgct ggcgccaaag gattgcgcgg gcgcgctttt gggcagggcc aggcccacaa
     2881 cccccgtgcc cagggcgcac aaacccaggc cgagcccctg gcccagcaca tgtctacgag
     2941 aaatattcag gcgaatcggc ttttccatgt aacctgtatt aaatgagcca cactagcatc
     3001 cgagtccagc cttactcggc agaagtacct gcttcagcgg cgagtcgttg cagggcgtcg
     3061 ccatccacgc gcatcactgt ccattcttcc atcggccggg cgcccagcgc gcgataaaag
     3121 ccgatcgacg gcgcgttcca gtcgagcacc gcccattcga ggcgcgcgca gccgcgtgcg
     3181 gtggccagcg ccgccagttg cagcagcagc gccttgccca ggcccgcccc gcgcgcttcg
     3241 gggcgcacga agaggtcttc cagatagagg ccggggcgcc cctcgaatgt ggaaaagctg
     3301 tggaagaaaa gggcaaaacc ctgtggaagc ccgtggattt cgccgatcag cgcctcggcc
     3361 atggggcggg gcccgaagag atggcggccc agttccgcct cgctgaagcg cagttcgtgg
     3421 gaaagccgct cataggcggc cagatcgcgg atgaactggc agatcagcgg cagatcggcc
     3481 ggggcggcag ggcggatggc aagcgtcatg ccggcggtgt gggcgcgcgg ggcagggctg
     3541 gcaaggggct cagctgcccg ggtcgagcac catcgtgtcg ccgcgcagtt ccacccgcgc
     3601 cagccgccgc agcgccgatt ggcccagcag gttgatcgcc gcgccctgga tcaccaccgc
     3661 atccacatcg gcaaagctgc gcccggccag caccagccgg tccagatgca caggcgcgcc
     3721 catcgccgtg cccgaggcgg tgcgcagcac cgggcggaaa tcggccggcg ccacgttgag
     3781 gcccagccgg ttggcctgcg cttctgtcag cgccactaca tcggcgccgg tatcgacgag
     3841 gaaatcggtg tcctgcccgt tcacttgcgc ggtgaggtgg aactggccgc gcccgtcacg
     3901 catgagggtg acagggccgc tgggctgggc ggggctgccc ggcggcggcg ggggcggggt
     3961 gttgccccag gcgccggcgc tctggtggaa gacatcgggc gtggcatcgg caaaggggtt
     4021 gggggccagg gaagtggagg cgccgggcgc gctggcgggc gggcgggcgt gcatcaacgc
     4081 ctgcgccagg gccatcagcc cgagaatcag cacaaaggtc cggaagagta gtcgccccat
     4141 ggggcaaata cttaatccgg caattgttat aaaaacgtag acgcattctg tctcaaggcg
     4201 ggccctctca gtgctggccg atcagttccg ctgcccgcca ggccaccgcc atggcggggc
     4261 cgttggtgtt gcccgaaatg ctggtgggca tgatcgagca atcgaccacc ctgaggccgc
     4321 tgaccccatg cacgcgcagc tgcgcatcca ccaccgcatc gccgccctct gccccgctgg
     4381 cgggccccat gcggcaggtg ccggtgccat gaaggcccgt ggacagcatc caccacagcg
     4441 cgcgggtgat ctcctcctcg ctggaaacgg cagggcccgg cacggtttcc tcgccgatat
     4501 aggcgcccag cgcttcctgg cggacaaaac cgcgaatcgc gcggatcatg cgctgggcgg
     4561 cggcgccatc gcgcgggtcg gccagccagc cggcatccac ctgcggcggg ctggccgcat
     4621 cggcagactg gatctccacc cggccccggc cgaaggggcg caggtagaaa gcggcaacgg
     4681 tgatgccggg caccttttcg ggcacgccgc ggccggcctc gggcttgtcg tcggcgctgc
     4741 tttccatcga atagggggca atgcccagct gcagatcggg ccagctggca tcgccggtgg
     4801 tggagatcat cgccgagaca tcgggcacca gccgcgccat cagcccggtg ccggtgagga
     4861 aatagcgcaa ggcattggca tagaggcgcc agccgcgaaa ttcgcggttg atgccggcca
     4921 tgcccttcag ccggaaggag agcgagacca tcatgtgctc gctcatattg gcgcccacgg
     4981 ccgggttgtt caccaccacc gggatgttgt ggcgcgccag cagctctgcc gggccaatgc
     5041 ccgacagttg cagcagcttg gggctggtga gcacgccggc ggccagaatc acctcgcccg
     5101 cgcggatttc ctcgccgctg gccagcagaa cgccggtggc gcggccctgc tcgatcagca
     5161 cgcgctgcac cagcgcgccg gtgcggatgg tgaggttggg ccggtgccgc gccttgccca
     5221 ggaaagcggt gaaagcggtg acgcggttgc ccttgcggtc caccgtcatc tgcgacaggc
     5281 caatgccggc ctgccccggc tgattgacat cgcccagcac cggcaggcca aactgttcgc
     5341 ccgcgcgcag cagggcgcgg gtgagggggg aatcgtcggt gctggtgatc acttccagcg
     5401 ggccgccctt gccgcggccg gcggccggcg ccgggccgtg gtaatcctcg atcgcggcgt
     5461 agcagcgttc gatctcggcc cagttccagc cggcattgcc ctgcgcttcc caggcgtcgt
     5521 aatccctggg ctggccgcga taataccagg tgccgttcac cgccgaggag ccgcccagcc
     5581 ctttgccgta ataccaggtt tcgccagccg ggcgctggcc ggcctgcttc accgggaagg
     5641 gccagtaata ccgcgggttc atccagatct tggccatgcc gcgcggcatg cgcaccatgg
     5701 tatcgcggtt ggcgccgccc gcttccagca gcagcacgcg cgccttgcca tcggccgaga
     5761 gtctttcagc cagcacgcag ccggcagagc cggcgccaac gatgatatag tcgtatgcca
     5821 tgcgaaggat ccttcaggcg cgcttttcgg tgccgcgcgg ataatcaggg gggaagcgga
     5881 acagcagcag ggcggccgcc atcgccagat aggaaagcgc cggcaggccg gcatggctcc
     5941 aggtgatggc gctgatcact tgcgggctgg ccggcaggcc cggggcataa tgggcgcgcg
     6001 ccagcagcca ggcgccggcc gccccgccga tgctcatccc cagcttcacc ccgaagctgg
     6061 aggcggcaaa caggaacccc tgcgcgcgaa agccgctctt ccacgcgccg aaatccaccg
     6121 catcggccat gaaggcaaac agcagcccgc ccagcaggcc cagcccggca taggccagcg
     6181 cgttcatcgc catcagccag gccagcgatg tgcccagcgg cagggcggcg gtgcagatgg
     6241 cgccaatccc ggcgccgatc atgccggtgg ccggcttgcc gatgcggttg gccaccaggg
     6301 cgctgagggc ggtgccgatc aggatcacca gcatcatcgc catcagcggc ggcgcccatt
     6361 cggcatggcc ggccacatcg cgggcataat agatggtgga ctggatatgg ccgatgaagc
     6421 cgatccagaa ggccaggttc accagcatgg tggcaatcca cggccaattg ccgcgcgtgg
     6481 ccagcagcga ttggcgggtg gtcaggcgct cggggctggc ctcgggggct tcgcgctcgc
     6541 gggtattggc aaaggcgatg gcaacgccgg ccagcgtcaa caccgccagc agcagggccg
     6601 cgcgcacaaa gcccgcctgt tgatcccccc cgcccagcgc gcccaccagc ggcagcatgg
     6661 catagctcac ccccaccgtg gccgaggagc cgagcagttc gcggatggca ttggtggtgt
     6721 tgcgcgcgcg cacgtcgcgc gtcagcgtgg gcagcatgga attgagcggc aggctgatgc
     6781 aggaatagag caggcccacc gctgtgtagc tgacatagat ataggccagc ttggccaggc
     6841 ccgacagcgc cggggcggca aacaccgcca cccccgaggc cgcaaagggc agggcaaacc
     6901 acaggagata gggccggtgc ttgccccagc gcgtgtgggt gcgatcgatc gccagcccca
     6961 gcagcggatc gaacagcgca tccaccccgc gcgccagcag gaacagcgag gcaaccgtgg
     7021 ccgggctgac gccatagacg tctgtgtaga aatacatgag ataggcgccg gtggccccga
     7081 agatgaggtt caccccgaaa ttgcccaggc catagctgac ccgttccttc agcgaaatgc
     7141 cggcgcccat ggcttgttgt cccttactgg ttggccggca ccggcagcgc gcccagaaag
     7201 gcgagcgagc ggcgcacttc ggccacagga tcgggcacgg catcgatttc cacgatgttc
     7261 cagccggcaa agcgggcatc tgtcagcagc gcctgccagc cggcccaatc caccttgccc
     7321 gcccccagcg cgcagaaata gggccggtgc gagaggtgca cctggtgatc gatggggatg
     7381 cgcacggggg cagggccggt ggcatccttc cagtgggtgg cgatgaggcg ttccttgtgg
     7441 cgcgccacca cttccaccgg attggcaccg cccagcagga tatgcgccgt atcggggcag
     7501 aagccgacga agaccggatc ggtgagcagc aggatgaggt cgatgtcacg cgccagcgag
     7561 aacagcgaat gggcctcggt gtgcagcgcc agttgcacgc catggcgcag ggtggcagcg
     7621 cctagcaggt tgcagaaatc ggcgatggtt ttggcggtgt cgaagctggc gatctgcggc
     7681 ggctggctgt cccagctggc gcgcatgggc aggcccatca ccatcgcgtc cacgccaaag
     7741 ctgcccagat attcggcata gaggctggcc tcggcgatat aggccgcgcg cgtttcggcg
     7801 gtttccagat cgccgccgat cgccacatcg gcgaagaagc cgctggccag ctgcaggccg
     7861 cgcgcggcca gcgcctcggc aaagccggcg gcagaaccat aggcggccag cgtgtcgcgc
     7921 cagccgaagg gtgagaaggt cagctcgatc ccgcgtgcgc cgctttgcgc cacggcatcc
     7981 agcatgcggt cccagaaggg gcgcggttcg gccttgccca gcgccaccac ttcgtcctca
     8041 ttggccagcc cccagaagga ggggtgaaaa aaagtgatga gatcaatcgc ataagcctgg
     8101 gtagtggtgg ccgaagaggg ggaagctggc attatcggtc ctgtctgcgt gcggcgcctg
     8161 gtggtgcgcg gcggttggcc ggggcagtgt ggggccgcgc ggccgcacta aaagaaatcg
     8221 tttacatatc tatgagccag ggttggccct gtcaagcaag tctcgcgcgc ggcgctgcgg
     8281 ggcgggggcg gggtgcgggt gtttcacagc ggtttcacgg ggatgccggc cgcgcccggc
     8341 cccacgcata cgttcccacc gggcgttttt gccattgcct gccgccgccg gcgatgcaaa
     8401 agctgtggcc aacaccaaaa ggccggggcc ggccgggtgc ggattgatga aatagtttac
     8461 attggccatg ccatggcctt ttccggccct ttgcgccaac cccaccgccg gcccggcaga
     8521 ttgagagaga tggaacacag atggccttgc agaccttcgc gctgatcggc ggcggcccgg
     8581 gcagcttcat cggcccggtg caccgcattg ccgccgaact ggatggcgag atgcggctgg
     8641 tggccggcgc cttcagctcc agcgcggaaa aatcggcggc ggcgggcgcg gcctatgggg
     8701 tggatccggc gcgggcctat gccagcatcg ccgatctcgt ggcgggcgag cgggcgcggc
     8761 ccgatcctgt ggatttcatc gccatcgcca cgcccaacca catgcatctg ccggccgccc
     8821 gggccgcgct ggaagcgggc ttcccggtca tctgcgacaa gccggccacc gccactttcg
     8881 cgcaggcgca ggaactggcg cagatcgtgg cgcgcgccgg cctgcccttt ggcctcacct
     8941 acacctattc cggctatccg ctggtgcgcg aggcgcgcgc gcgcattgcc gccggcgccc
     9001 tgggcgagat ccgcaaggtg gtggtggaat atccgcaggg ctggctggcc aagccttcgg
     9061 aaggcaagca ggcggcatgg cgggcagacc ccagccgctc tggccccggc ggctgcgtgg
     9121 ccgatatcgg cgtgcatgcc ttccatctgg ccgagttcat ctcgggcctg caggtcagcg
     9181 agatcctcgc cgatctgggc acggtggtgc ccggccggca ggtggacgat gactgccaga
     9241 tcctgctgcg ctttgccaat ggcgcgcgcg gcgccctgct ggccagccag atcgccgtgg
     9301 gcgagcgcaa cgggctggcc atccgcctct atggcgagaa ggccggcctt gcctggcggc
     9361 aggaagagcc caaccgcctg tggctttatc acgaggatgg ccgcagcgag ctgatccagg
     9421 cgggcgatcc cggccttggc gccgatgccc tgcgcgccag ccgcacgccc ggcggccacc
     9481 cggaaggcta tctggaggcc tttgccaata tctaccgcga ttttggccgg ctgctgcgcg
     9541 gggaagcggg cgcgctgatc ccgggcatgg ccgatggcct gcgcggcatg gcgctgatcg
     9601 aacaggccgt ggcgctcagc cgcgccggcg ccggctggac cgccttcacc gtctgatcgg
     9661 gagagacagg atgcgtaatc tcaaggggcc gggcctgttc ctggcgcaat tcgccggcga
     9721 caacgccccc ttcaacagcc tttccgccat tgccgactgg gcagcggggc tgggctacaa
     9781 gggcctgcag atccccagct gggatgcgcg cctgttcgat ctggccaggg ccgccgaaag
     9841 ccagacctat tgcgacgaga tcaccggcat gctggccgaa aagggcctgg ccatcaccga
     9901 gctgtccacc catctgcagg gccagctggt ggcggtgcac ccggcttatg acgcgcagtt
     9961 cgacggtttt gccgtgcctg aagtgcgcgg caacccggcc gcccgccagg cctgggcgac
    10021 cgagcaggtg aagctggcgg cggttgcctc gcgccggctg gggctggcgg cgcatgcctc
    10081 cttccccggt gcgctggcct ggccctatgt ctacccttgg ccgcagcgcc cggccgggct
    10141 ggtggaagat gccttcgacg agctggcccg ccgctggcgg ccgattctcg acgtgttcga
    10201 cgagaatggc gtcgatatcg gcttcgagct gcatccgggc gaggatctgt tcgacggcgt
    10261 caccttcgag atgttcctcg agcgggtgga gaaccatccg cgtgcgaaca ttctctacga
    10321 tcccagccat ttcgtgctgc agcacctcga ttacctcgct tttatcgaca tctatcacga
    10381 gcgcatcaaa tgcttccacg tgaaggatgc agagcttcgc ccttcgggtc gctccggggt
    10441 gtatggcggc tatcagagct gggtggaccg gccgggccgg ttccgcagcc tgggcgatgg
    10501 ccaggtggat ttcgcgcaga tcttcagcaa gatggcccag tatgactatg ccggctgggc
    10561 ggtgctggaa tgggaatgcg cgctgaagca cccggaggca ggtgccgctg aaggggcgcc
    10621 cttcatcgcc cgccacatca tccgcgtgac cgaacatgcc ttcgacgatt ttgccgcagg
    10681 cggcgctgat cgcgcgctga acaagcggat catgggcatc gactgacgcg ccgggcgatc
    10741 ggccaagcga ttcagatcaa ggcagggccc gccggccgtg ctatggctgc ggcgatgacg
    10801 aaagcgccgc gatcatgact tttgccggcc ttggcctgat gggcgggctg gcgctgggtt
    10861 tggccagcag cctgcattgc ctgggcatgt gtggggggat tgtctcctcg ctgatgctgg
    10921 cgggcggcgg gcggcgggcg cctggctggg cagggcggct ggcgcggctg cagggcgggc
    10981 gcgtgctggc ctacagcctg ctgggcgcgc tgtgcggcgg gctgggcggc gcggtgtttg
    11041 gccatctggc ggggccggtg ctgtttctgg tgctgcgttc ggtggcggcg ctgtcgctgg
    11101 gctgggtggg gctcacgctt accggccttg cgccgcgcgt gccgctggcc gaacgcgggc
    11161 tggcgctggg cgcggcgggg ctgcggcgtg tgcaggcgct ggtgccggcg gtgctggcgc
    11221 cggtgctcac cggcatgatc tgggggctga tgccttgcgg catggtctat ggcgcgctgg
    11281 cctttgcgct ggtcagtggc tcggcgctgg gcggcgggct ggtgatggcg ggctttggcc
    11341 tgggcacgtt gctgccggtc agtgcggcgg gggcaggcgt ggcctggctg ggccagcggg
    11401 ccaccggccc gcgcatgcag gcggcgctgg gcgtggtgct gatgggcggg gcggcggcct
    11461 cgctctgggt ttcgccggag gctgtgcgcg cgctgtgcgg cggctgagcg gcggttttcg
    11521 gcgccgtttc gcaccagaga tggcccgccc gcgcggttga taagccttgc gaaaagcgct
    11581 gccgcccggt tggcccgggc cagacagggc ctagttgcat ccggaatgaa tgtgttttcc
    11641 ccgcagaatg atagatatct gcgcccggac tgataataac aagacgggat catcggggga
    11701 cttatgcaag gacggcgcgt cgtttccggc ccggtggcgg gctgtgtgca agcatctttg
    11761 cgtcatcaca ccgatgacgt tggcaaatgc gattcccgat atgcgctttc agagcgggtt
    11821 gatatttgta tctgcacctt caggcggagt gatttgtata aaacgctgga gtccatctgc
    11881 catcagattg acacagataa tttacaattg aaagtcatta ttatcgacaa tgacgatgtc
    11941 gagagcgccc ggccggtggt ggacagggcg atcgcccgcc tgaaaatcga ttgtctttat
    12001 ctccacgcgc cggcccgcaa tatatccttg gcgcgcaatg ccgcctttgc cgccaccagc
    12061 gcgccctatg tgctgtgcat cgatgacgac gaggtggccg cgccccgctg ggtgcggcac
    12121 atggtggatg ccttgcggca atcggcggcg ggcgtggtga tcggcccggt gcatgccgtc
    12181 tatagcccgc accacccgcg ctggatgcgc gagatggagc cgcattcggc catcccgcgc
    12241 ggcaattacc gtgccgcgcg cctcacccat atcggcaatg ccggctttcg gggcgcggtg
    12301 atccgggggc ggcgcttcga tctcctgttg gggcgcagcg ggggggagga taccgagttc
    12361 ttctcgcgcc tgcgccgcga agccgtgccg atcacctatt gcgacgaggc caaggtgttc
    12421 gagccggtga gcgaggagcg ggccagcatg aaatggctgg tggaacgcca cttccgcatc
    12481 ggccagacct atgcccggtc ggagcggccg gcaggcggcg ggcggctgcc gctggcggcg
    12541 cgggcggcgg ccaaggcgct ggcctgcctg gggctggcgg cggccaatct gggccatgaa
    12601 gggcgggcca agtgccattt cctgcgcttt gtcatgcatg ccggcgtgct gatgaacgcg
    12661 ctgggcctgc cgccgatcga attgtattga ggggggcggc ggatgagcct ttcgctggcg
    12721 cagaagctgt cgttcaggct ggccacgggc tggcagcagc gcaccatggc gcgggcggtg
    12781 gcagggccgg tgctgagcat cggtttcgac gatgcgccgc gcagcgcctg gaccatgggc
    12841 ggccaggtgc tggccgatca tggcgtgcgg gccacctatt atgtgtgcgg cgggctggtg
    12901 gatacggatg gggagctggg cacctatctc acccgcgcgg atctgcgcgc gctggtggcg
    12961 gccgggcatg agctgggttg ccacacattc gatcacagca gcgccctgct ggcgggggcg
    13021 ggccggtttg ccgcctcgct ggaagagaat gcccgctggc tggcggattt caccggcggc
    13081 gtggcgccgt gcagctttgc ctatccctat tgccatgtgg ggctggcggc gcggcgggcg
    13141 gcgggcgcgc gtttccacac cgcgcgggcg ggcgggcggg cgtggaacgg gcccgtgttt
    13201 gatccggccg tgctggccgg ctggccgctg gaagcgcgcg agggggcggt ggattatgcc
    13261 gacctgtgtc aggcggcggc gcgggcgggc ggctggctgg tgctgttctc gcatgatgtg
    13321 agcgagcggc ccggccccta tggctgcacc ccccggcagc tggacgaggt gctgcgcgcc
    13381 gcgcgccggg cgggcatggc gatcctgcca gcgggcgatg tggcgctggt gcggcgctga
    13441 gcgttttttt cgcgaacatg tcttgtgtgt tgcagggcgc gcgcggccgg gcttggcgat
    13501 gggcgtgcgc aaggcgtgtt tttcgcacaa gcgaggtaac gatgtcggat tgtatctaaa
    13561 gtgtatccgc tacaaatgta aaaaaacgta caatactgaa aattttcggt tttatctgtg
    13621 gaaatctacg gtttccgcgt caaactatta tagtttcatt aaggacattt acgtagcacc
    13681 tgggcgtgcc gaggcgacat taggcataag acaaatgacc agcaggggtt attatgccta
    13741 aggtaaaagc gcgccttctt tcgacgtcca ccatggccgt tagccttgtg ctggctggca
    13801 ttggcactaa tgcactagct gattcagcga ctacttctac agatggtaat accgttgtcg
    13861 tggatggcaa tggaaccacg ggctatgtta ttacatcggc gttgagcaca tcctccgcca
    13921 atcccactgt catcaatggc tcgtcttcca gctcggtcat ttcagtgagt ggcaccaatg
    13981 ttacggtggg ctccagcacg accaacacgc tgttcaccga tttccacacg gtgggcggct
    14041 cgggcagcgg cggcggcgcc gggctgggcg gcgtgttcta tgtcgattcc ggcgagagcc
    14101 tgacgctggt caacgtctcc ttctccagca acctagccca gggcggcacc ggcggcactt
    14161 ccaccacggg cggctcgatg aacggcctgg tgacgacttc gagcggcacc agcggtgctg
    14221 ccggctcgga tgccgcctcc gattattcca gctacaacag cacgaatggc ggcccgggct
    14281 atgccgggca taccggttcg gcagcgtcca gcggcgtggg cggcaatggc ggcaagggcg
    14341 gcaatggctc gagcggctcg acctcgtcgg atgacatcat cctgtcggcg ctgacggcgg
    14401 cctacgatct cttcaaggcc ggaaccacat cggatacggc cgaaacctac agcacctttg
    14461 ccgaagagtt ctcggctctg gcgatcgagg cgtcttcggg ggcgaatgcc ggcgggccga
    14521 ccaccgagac gctggtggcg ggcctggaag ccatcgcggc cactttcacc aagctgtcca
    14581 ccgagaccgc ttcgcaagcc agccaggatt atgagcagga ggccgcgcag gaggcctatc
    14641 agatcaagct gacgatggac gcctacacgg ccggcgcttc gggcaatggc ggtgccggtg
    14701 gtgccggtgg caatgcaggc gcgggcagca ccttctttgg cggcggcgat ggcggcaatg
    14761 gcggcaatgg cggcaatgcg gtgtcttcca cagggacgac cggcggcggc atcggcggcg
    14821 atggcggtga tggcggcacc ggcggcaatg gcggctttgg cgccggcggc ggttcgggcg
    14881 gcacgggcgg cacgggcggc acgtcggcct cgagcgacaa tgtgaacggc gcggacggtt
    14941 cggatggcgc tggcggcacg ggcggctttg gcgccggcga cggttccacc ggcagcgaga
    15001 aaaacagcga tggcacctat ggctcgggcg gcggcggcgg ctctggctat ggcggcgcga
    15061 tcttcgtgcg cagcggcggt tcgctgacgg tgacgggcaa ctccaccttc tacgacaacg
    15121 ccaccgaggg cggcagcagc gagaatggcg gcgcggcggg cgattcggcc ggcagcgaca
    15181 tcttcatgat gaagggctcc accgtgaccc tggccccggg cgtgggcaac accatcacct
    15241 tctacggcag cattgccgac gacagcgccg ccagcatcga cgatgcctcc tatgcggccg
    15301 gcaatggcgc cagcctcatc gtcaccggcg gcggcacggt gaagttcctg ggcaccaaca
    15361 cctacaccgg caccacctac atcgacggcg ccacgctgga agccgacaat ggcgtgggca
    15421 tcgacacgct gagccatgtg gccttcaacg gcaccggctc gatcaacaac agcctctcca
    15481 cgctggatgc cggcgtgtgg ctcaccagcg gcacgctgac gcgccgcgtc ggcacgctcg
    15541 acacgcagat ctcgtggggc ggcagtggcg gctttgcggc cacttcggcc ggcctcacgc
    15601 tgaacttcgg ctcgatcaac ggcggcctcg gccagacgct gacctggggt tcgggcagct
    15661 tcgtgccggt gggcagcacg ctgatcttcg gttcggatgc caccgatgcc accggcgttg
    15721 tcaccctgct caacaacatc aacctcaatg gcaaccaggg ccagatcgcc gtttacaaca
    15781 atgcctcggg cacggcctat gcggtgatct cgggcaatat ctccaacggc acgctgctgg
    15841 tgggcgatac cgattattcc ggcacgctct acctcaccgg caccaacacg ctgtcgggcc
    15901 tgacggtgca gaacggcacg gtgtccaccc tgctgggcac caccgtgggc cagctgatgg
    15961 atcccaccaa cggcggctat ctcaccatca ccggcggcca ggtgatcctg ggcggcgcgg
    16021 aaaagctgac cagcgtcaac cttgccagcg gcacccagct gaccgcctat ggcgcgctga
    16081 cgaccaatga catcaccaac gtcggcacca tcctcgacaa gagcacgctg aacgcgggca
    16141 ccatcaccaa caccggcagc ctgacggttg acggcgcggt gaccagctcg tggatcaaca
    16201 acaccggcac tatcgatttt gccgtcaacg ccaccaccgg cgccatcacc aacagcagca
    16261 ccggcgccat caccgtgggg ggcaacctcg tctcgggcgc tattggcaac gatggttcgg
    16321 tcatcgtcga tggcaccgcc aacaccggcg ctgtcaccaa caccggcacg ttcggggtga
    16381 agggcaatgc caccaccggc gcgctctcga acagcggcac gatggtgttc gatgccaacc
    16441 tcaccagcgg cgccatcacc aacagcggca cgttcagcgt cacgggcaat gcctccaccg
    16501 gcgcgatcga caacaccgcc accggcaatg gcgtgatcgg cggcacgctg accaccaatg
    16561 gcgccaccct catcaatgat ggcaccttct cggtgggtgg caatgcggcc actggcaacg
    16621 tgaccaacag cggcacgctg acctttgcca gcaccgccca gatcggcacg ctgaccaaca
    16681 gcggcactgc ctatctggat ggcaccacca ccagcggggc gatcagcaac accaacctcc
    16741 tcgtccagaa cggggcggtc accgtttcgg gcacggtgag caacagccag ggcgccagct
    16801 gggtgctggc ggccgacctc accagcacca gcaccgtgac caacgatggc acgctgtggg
    16861 tcgtgggcga caatgacgac agcacggcgg ccacccgcac catcaccacc accggctttg
    16921 gcggcgccag caccggctat gtggggctgg gcgggctgga cggcacgctg gccaacacgc
    16981 tggtgatcga ccagtcgggc aacagcacct atgccggcgt cttctcgggc gcaggttcgc
    17041 tcgtcaagga aggcgcgggc acgctgacgc tgaccggcgc cagcgacttc accggcgggc
    17101 tgaccgtggc cgccggcacc atcgacacca ccggcggcgg cacctttgcc gacacgctgg
    17161 cgatcacggt ggacaagggc gccgaactga tcatgggcac ggccgatctg gtcggctcga
    17221 tcaccaatgc cggcaccctg ctggccacgg ccaacctggg cctcaccacg ctgaccaaca
    17281 gcggcacggc caccatcgat gcggtgctcg ccgcctcggg caatgtcagc aatacggcgg
    17341 atggcacgct taccctggcc tccaccgcca atgcgcagat cgccggcacg ctgaccaacg
    17401 ccggcacgct gaccaacaat ggcacgctct atgtcgatgg cgctgtcagc aacagcggca
    17461 cgctgacgct ggccagcggc agcaccaccg aactggccag cctgaccaat accggcacga
    17521 tcaccgccta tgccccgctc accgtggtgg gcagctacac ccagaatgcc ggtacgctga
    17581 ccgcctatga cggcctctac accggcacgc tgtcgggcag cggcggcacc atcaacctgg
    17641 ctggcagcac ctacacgctg aaccagaccg ccaacggcac ctattcgggc agcatcgtgg
    17701 gcggcggcac ggtggacaaa tacggcaccg gcacgctgac gctgaacggc gcggccgaca
    17761 gctttgccgc ctcgcagctg aacatctatg ccggcgatgt gaccgtggcc accgccaacc
    17821 tgctcgacag cgcgctcaac gtgctgatcg acacggccgg cacgctgacc ctggaagcca
    17881 accagacgat ccacgacctg accggcgccg gcacgctcga catcggcacc tcgacgctga
    17941 cgctgagcga tggcggcgag ttcagcggca cgatcgacgg caccggcacc atcgatgtga
    18001 cggatggcaa cttcacgctg aacagcgcca tcaacaacac cggcggcacc ttcaccgtgg
    18061 gcaccgacag cactgtcacc gtgggtgcca gcggcagcct caccaccacc aacctcaatg
    18121 tcaccggcgg cacgctggac ctcatcggca aggccacggc caccaccacc acgctgaccg
    18181 atggcgccac gctgcacctg ggcaatggcg tggacctgga cgagagcggc gccgaaaccg
    18241 gcacgctcac cagcaccacc acctatgtgc tgggcggctc ggcgatcacc ggcaacggct
    18301 cgatctcggg cacggtctat gtcggcgggg ccagcgcggg cattgtcgcg ccgggcaatt
    18361 cgccgggcat cctgtcgttc gacaacatca ccttcggcaa taattccacc gccgaaatgc
    18421 aggtggatgg cacggccggg gcgggcgtta ccggcggcaa cgacctgatc gaggtctccg
    18481 gccagctcac cctcacgggc agctccaccc tggcgatcag caagtcggtg tcgaacagct
    18541 tcgccctgtc gctgggcgag cagatccagc tgttcaagtt ctcggccggc aaggtgaagg
    18601 gctatttcgg ctcggtcacg ctcagcgact atagcggcag cgcgctctac aacgttgcca
    18661 ccggcacgct gatcggcctt ggcagctaca cgccggccag cttcaaggcg gcggtttcca
    18721 ccaccgccaa ccaggcagcg atcatgaagg cgctgatggt gaacgatgcc ggcggcgtgc
    18781 cgcagtacta tggcggcaac ctgatgagct atgtcacgtc ggcgctggcc agcaatccgg
    18841 catcggtggc cggggtcttc gcccgctggt cgcccgaggc ctatgccggc atcatggacc
    18901 agatgaagtt cagcatgctc gacaacctgc cggaactggg cggttacgac aagctgacgc
    18961 cgggccgcac ctatgccacc ggcagcttca accgcggcgg gctgaagggc gagggccaga
    19021 ccggctatgc ccagaacaac ctgcgtgacg atggctataa cgtgggcatt gcccatcagt
    19081 tcggctttgg ccagctcacg ctgtcctatg gccattcgga tggcaatttc tacggcacct
    19141 acatcgcctc gcgcacccgg ggcaatcagg tgggcctggg catctcggcg ccggtggcgc
    19201 tgggccaggc gctgcgggtc accggccgcc tggcctatgg cgactacaac tcgcatggca
    19261 cgcgcagcac caacagcggc acggccgatt tcgccggggt gaagagcaac atcttcgtct
    19321 atggcgcggg gctggaatat ctgaagcagg caggccagat ccgggtggat accacggccg
    19381 aattcctcgg catgcatgag cgcatgagcg gcttcacgga atatggcgcg gccgacacgc
    19441 tcgaccagat gagcgtggag cgcagcaccc acgaggccta tctggcccgc ctgggcgcca
    19501 ccgtgggcta cacggtgtcg ccgctggtcg aagtctatgc caagggcacc tacgaccatg
    19561 agttcggcca ccagatgacc gacatcaccg cccatgtcgc tgtggaggac accgccttca
    19621 ccgtggccaa caccggcctt gcccgcgacc gggtgagcgc tggcggcggc gtgaagttca
    19681 acctcacgtc gcgcctccag ctcaacctcg atgcgagcgc gggcaccaac gccagctatc
    19741 gctataacgc ggcggtgcgc ttcggcttct gatcccggcg cacgcaagaa aaggcccggt
    19801 cgtgcatgca cggccgggcc tttttgcgtc tggtgggggc gttcagtgcc gcttcatcgg
    19861 gccgggcgcc tgatggccgg cagacaacaa gaggacgagg agagggaatc atgcctgacc
    19921 ggatggcaag gatcgcgctg gccctgctgg caggcgcgct ggcggtgccg gccgcccacg
    19981 ccgacccaca gccgacaacc gaggctgccg ccccccgcga tgcccatacg ggccttgcta
    20041 caggcaaacg gcagcacgag cctgtgagca tccacaagac ggttgatgcc gccacgccca
    20101 aagcgatggc aagcccctcc accccggccg acactgccaa ggtgaaggcg catagcaata
    20161 ccaacaataa ttaggacggc tggcctgcgg cacaggcgca aggcccagcc cggcgcacag
    20221 ggcaagcctt gcgcgggatt atccacgtgg ggatagggcc ggatatgcag gaagagtgtg
    20281 gatgccccgc gaggattcga acctcgatag acggaatcag aatccgtagt cttgccgtta
    20341 gacgacgggg catcatcggg agagcggccc tctatgacgg cgaatcggtt tcgtcaagcc
    20401 cttgtcgaca gggcgttccg cggggcgttc ccggcgccct tgccccgcag ccccgggagc
    20461 gctagcatag aggggaaatc tccgccgatg gggcggctga acatctattt cgagtaagca
    20521 agatggcgga tcctcatccg ccggcatcgc gccataaggc gaaggggcac aagggccgca
    20581 ggaagcgaca gtcgcgccgc ggatcagccc agcccccagc cgggcctcag gccggtggaa
    20641 gcgctggcgc cagtggtgcg ccggcggcaa agacgcgtcc cggcgcggcc ggtggcggcc
    20701 gcaagcc
//
LOCUS       DASRYE010000070         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_4010,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000070 DASRYE010000000
VERSION     DASRYE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFF98_03985"
     CDS             complement(<1..366)
                     /locus_tag="VFF98_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZU62821.1"
                     /translation="MRHDRFHGRVRDAGRACQWPGCEEGGEFRAPGPQASGFDGPGAY
                     RWFCLPHVRAFNEGYDYFAGMSAEQILAAQHPVAGWDREARAFSPTAGVDAAPRWADY
                     ADPLEAIAARAADFRRQRQA"
     gene            420..740
                     /locus_tag="VFF98_03990"
     CDS             420..740
                     /locus_tag="VFF98_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZU62822.1"
                     /translation="MTTYTPGYTPGHAPTEGPLAREMRAALEAAFTPAHLAIINDSHH
                     HRGHVGDDASGESHFTVEIEAAAFAGLSRLARQRAVNAALGDIPGGRVHALAIRARAP
                     GEEA"
     gene            803..1567
                     /locus_tag="VFF98_03995"
     CDS             803..1567
                     /locus_tag="VFF98_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZU62823.1"
                     /translation="MMMGIFPGFGLIIGFFALVLVLKSFGINQEYQRGVVFRLGRLAE
                     TKGPGWFWLIPVIDRVVKVDIRVITSALATQETVTRDGVAVKVNAVLWYRVVDAARVV
                     TSVMYWDAAVIQAAETGLRDAIGQSDLDQLLKDRAAINGRLLEMLGVTCQQWGVVVDA
                     VEVKDLDIPEQMQRAIAREAEAIREKRARIIKAEGENVASNMLAEAARTISNSPGALE
                     LRRLQTISEIGAEHNSTIIAMFPVELLEAAKKLAEK"
     gene            1564..2472
                     /locus_tag="VFF98_04000"
     CDS             1564..2472
                     /locus_tag="VFF98_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU62824.1"
                     /translation="MKAPRPVRLAGGITLDVWEEGPEQAPALLFLHGFPESGRAWRHQ
                     IAHLAGRYRCIAPDQRGYGGSSKPAAVADYALPALAGDVLALAEALGIARFALAGHDW
                     GGMVAWAAAIADAARPVAERRIERLVIANAPHPWLFARLLWLDAQQRRASAYIPQLCS
                     PAIDGVLATQGLGPVLLRAFGDGALAAMEVDERRALFARWADPAQALAMVNWYRASGV
                     VVPPAEAPLALPADWQQPAAPTLGLPTLVLWGEADTALVPANLAGLADLCTDLAIERL
                     PGVGHFSPWQAPAAVNAALDRFLLGG"
     gene            complement(2469..3536)
                     /locus_tag="VFF98_04005"
     CDS             complement(2469..3536)
                     /locus_tag="VFF98_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HZU62825.1"
                     /translation="MDIGKAEGRAQAEAAIQWQAQHCDTMQAPLTAGVLRALLPLLAG
                     GSACAQRMRSWPQLTSADAMPLRLAGGLHHLHLSGAEAGLGPVYRGELADQAGIDAAV
                     AAAVARHDAALLRWFDSPPQTNEAGRSASLMAGLMWLAARLGPRFELNEIGASAGANT
                     MIERYAYDLGGVRCGPAASPVLIRPEWRGAPPPAASPDILHIEGCDQAPVNLADPAEA
                     LRLRAFVWAENTQRMARLEAVAALASARPPRLARADAADWVAARLATAQPDDSTRVLY
                     HSIVWQYLPEPTKARITAAMEAAGARATPARRLAWLRLETNRTTFRHELRLRIWAGAG
                     EEEEAFLATAHAHGAWVEWLA"
     gene            3616..4299
                     /gene="hisG"
                     /locus_tag="VFF98_04010"
     CDS             3616..4299
                     /gene="hisG"
                     /locus_tag="VFF98_04010"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZU62826.1"
                     /translation="MSVSQSFAGITFAVPKGRILEEALPLMARAGVVPEADFHNPKSR
                     ALAFACEGADMRLIRVRAFDVATFVAHGAAQVGIVGSDVIEEFAYADLYAPVDLNIGH
                     CRLSVAEPVEKPVAAAAASHLRVASKYPNLTRRHYERQGIQAEVVKLNGAMELAPGLG
                     LSGRIVDLVSTGRTLKENGLVETDQIMQISAHLIVNRAALKTDRRVGALVEAFRALVA
                     QGAAEEAGA"
     gene            4296..>4681
                     /locus_tag="VFF98_04015"
     CDS             4296..>4681
                     /locus_tag="VFF98_04015"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZU62827.1"
                     /translation="MIRLDSRAAGFAEAFARIVADRRESDGDVSSSVTAILADVRARG
                     DDALAELTQRFDGYALASEDDWRITPAACRAAYDALEPALRTALDTAAARIRAYHAAQ
                     LPENRDYTDAAGVRLGAVWRAVDAAGL"
BASE COUNT          707 a         1625 c         1640 g          709 t
ORIGIN      
        1 ggcctgccgc tgccggcgaa aatccgccgc gcgggcggca atcgcctcca gcggatcagc
       61 ataatcggcc caacggggcg ccgcatccac cccggcggtg ggcgagaagg cgcgtgcctc
      121 cctgtcccac ccggccaccg gatgctgggc ggcgaggatc tgctcggcgc tcatgccggc
      181 gaaatagtca tagccctcgt tgaaggcgcg cacatgcggc aggcagaacc agcgataggc
      241 ccccggccca tcaaaccccg aagcctgcgg gccgggcgcg cgaaattcgc cgccctcctc
      301 gcagccgggc cattggcagg cgcggcccgc atcgcgcacc cgcccgtgaa aacgatcatg
      361 tctcatcttc cttacatggc gacagatggg ctagatgaaa agccactgga gacgagaaga
      421 tgaccaccta tacccccggc tatacccccg gccatgcccc cacagaaggc ccgctggccc
      481 gcgaaatgcg cgccgcgctg gaagccgcct tcacgccggc ccacctcgcc atcatcaatg
      541 attcgcacca ccatcgcggc catgtcggcg atgatgccag cggcgaatcg catttcaccg
      601 tggagatcga ggcggccgcc tttgccggcc tttcccgcct ggcccgccag cgggcggtga
      661 atgcggcgct gggcgatatt cccggcgggc gcgtgcatgc gctggcgatc cgcgcccgcg
      721 cgcccggtga agaggcataa ggcagacacc gaactgacac cggcccggcc taaaccggcc
      781 ccgcaaacgc cgaggagacg tgatgatgat gggaattttt ccgggatttg gcctgatcat
      841 cggctttttc gcgctggtgc tggtgctcaa gagcttcggc atcaaccagg aatatcagcg
      901 cggcgtggtg tttcgcctgg gccggctggc agaaaccaag gggccgggct ggttctggct
      961 gatccccgtg atcgaccggg tggtgaaggt ggatatccgc gtcatcacct cggcgctggc
     1021 cacgcaggaa accgtgacgc gcgacggcgt ggcggtgaag gtgaatgcgg tgctgtggta
     1081 tcgcgtggtg gatgcagcgc gcgtggtcac cagcgtgatg tattgggatg cggcggtgat
     1141 ccaggcggcc gaaacgggcc tgcgcgatgc catcggccaa tccgacctcg accagctgct
     1201 gaaggaccgc gcggcgatca acggccgcct gctggagatg ctcggcgtca cctgccagca
     1261 atggggcgtg gtggtcgatg cggtggaagt gaaggacctc gacatccccg agcagatgca
     1321 gcgcgccatc gcccgcgagg ccgaggcgat ccgcgagaag cgcgcccgca tcatcaaggc
     1381 cgagggcgag aatgtggcct ccaacatgct ggccgaggcc gcccgcacta tcagcaacag
     1441 ccccggcgcg ctggaactgc gccgcctgca gacgatctcg gaaatcggcg ccgagcataa
     1501 ttcgacgatc atcgccatgt tcccggtgga actgctggaa gcggcgaaaa agctcgccga
     1561 gaagtgaagg cgccgcggcc cgtccggctg gccggcggca tcacgctgga tgtgtgggag
     1621 gaagggcccg aacaggcgcc cgcgctgctc ttcctccacg gttttcccga aagcgggcgg
     1681 gcctggcgcc accagatcgc ccatctggcc ggccgctatc gctgcatcgc gcccgatcag
     1741 cgcggctatg gcggatcctc gaagccggcg gcagtggccg attatgccct gccggcgctg
     1801 gcgggcgatg tgcttgccct ggcagaagcg ctggggatcg cccgctttgc cctggccggg
     1861 catgattggg gcggcatggt ggcctgggca gcggccatcg ccgatgcggc aaggcctgtg
     1921 gcagagcggc ggatagagcg gctggttatc gccaatgcgc cgcacccctg gctgtttgcc
     1981 cgcctgctgt ggctggatgc acagcagcgc cgggccagcg cctatatccc gcagctgtgc
     2041 tcgcccgcga tagacggcgt gctggccacg cagggcctgg gcccggtgct gctgcgcgcc
     2101 tttggcgatg gcgcgctggc cgccatggag gtggacgagc gccgcgccct gttcgcccgc
     2161 tgggccgatc cggcgcaggc gctggcgatg gtcaactggt atcgcgccag cggcgtggtg
     2221 gtgccgccgg ccgaagcgcc gctggccctg cccgccgact ggcagcaacc cgctgccccc
     2281 acgctgggcc tgcccacgct ggtgctgtgg ggcgaggcgg atacagcgct ggtgccggcc
     2341 aaccttgccg ggctggcgga tctgtgtacc gatctggcca tagagcgcct gcccggtgtg
     2401 ggccatttca gcccctggca agcgcccgca gcggtgaatg cggcgctgga ccgcttcctc
     2461 cttggcggct aggccagcca ctccacccag gcgccatggg catgggcggt ggcaaggaag
     2521 gcttcctctt cttcccccgc ccccgcccag atgcgcaggc gcaattcgtg gcggaaggtg
     2581 gtgcggttgg tctccagccg cagccaggca agccgcctgg caggcgtggc ccgggcgccg
     2641 gcggcctcca tcgcggcggt gatgcgcgcc ttggtcggct cgggcagata ttgccagacg
     2701 atggaatgat agagcacccg cgtgctgtcg tccggctggg cggtggccag gcgggcggcc
     2761 acccagtctg ccgcatcggc gcgcgccaga cgcggcggac gggcgctggc cagcgccgcc
     2821 accgcttcca gccgggccat gcgctgcgtg ttctccgccc agacaaaagc acgcaggcgc
     2881 agcgcctcgg ccggatcggc caggttcacc ggcgcctgat cgcacccctc gatatggagg
     2941 atatcggggc tggcagccgg cggcggggcc ccgcgccatt ccggccggat caacaccggc
     3001 gaggccgccg gcccgcagcg caccccgccc agatcatagg catagcgctc gatcatcgtg
     3061 ttggcgccgg cgctggcgcc gatctcgttc agctcgaagc gggggccaag gcgcgcggcc
     3121 agccacatca gcccggccat gagcgaggcc gatcggcccg cctcattggt ctgcggcggg
     3181 ctatcgaacc agcgcagcag cgccgcatca tggcgcgcca cggcggctgc caccgccgca
     3241 tcgatgcccg cctgatcggc cagctcgccg cgatagaccg ggcccagccc cgcttccgcg
     3301 cccgagagat gcaggtgatg caggccgccg gccagccgca gcggcatcgc atcagcgctg
     3361 gtcagctgcg gccagctgcg catccgctgc gcgcaggcac tgccgcccgc cagcagcggc
     3421 aacagcgcgc gcagcacccc tgccgtcaac ggtgcctgca tcgtgtcgca atgctgcgcc
     3481 tgccactgga tggcggcctc tgcctgtgcg cgcccttcgg cctttccgat atccatgatc
     3541 gcctctttgc gtgagatcgc ttctttgggg ggcaggcggt tgccctttgg ccccgccacg
     3601 cgtaatgccc ggcgcatgtc tgtttcgcaa tctttcgccg gcatcacttt tgcggtgccc
     3661 aagggccgca tcctggagga ggcgctgccg ctgatggcgc gcgcgggcgt ggtgcccgag
     3721 gccgatttcc ataaccccaa atcgcgcgcg ctggcctttg cctgcgaagg ggcggacatg
     3781 cggctgatcc gcgtgcgcgc gttcgatgtg gccacctttg tggcccatgg cgcggcgcag
     3841 gtgggcattg tcggctccga tgtgatcgag gaattcgcct atgcggatct ctacgcgccg
     3901 gtggatctga acatcggcca ttgccgcctg tcggtggcag agccggtgga aaagccggtg
     3961 gccgccgctg ccgccagcca tttgcgcgtg gccagcaaat atcccaacct cacccgccgc
     4021 cactatgaac gccagggcat ccaggccgag gtggtgaagc tgaacggggc gatggagctg
     4081 gcgccgggcc tgggcctgtc gggccgcatc gtcgatctgg tctccaccgg gcgcacgctg
     4141 aaggagaacg ggctggtgga aaccgaccag atcatgcaga tctcggccca tctcatcgtc
     4201 aaccgcgcgg cgctgaagac ggaccggcgc gtgggcgcgc tggtggaggc cttccgcgcg
     4261 ctggtggcgc aaggggcagc cgaggaagcg ggcgcatgat ccggctggac agccgcgcgg
     4321 ccggctttgc cgaagccttc gcccgcatcg ttgccgaccg gcgcgagagc gatggcgatg
     4381 tgtcgagcag cgttaccgct atcctcgccg atgtgcgcgc ccgcggcgat gacgccctcg
     4441 ccgagctgac ccagcgcttc gacggctatg cgctggccag cgaggacgac tggcggatca
     4501 cgcctgcggc ctgccgcgcc gcctatgacg cgctggagcc agccttgcgc accgcgctcg
     4561 acaccgccgc ggcccgcatc cgcgcctatc acgccgccca gctgccggaa aaccgcgact
     4621 ataccgatgc cgccggcgtg cggctgggcg cggtgtggcg ggcggtggat gcggccgggc
     4681 t
//
LOCUS       DASRYE010000071         9932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_161365,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000071 DASRYE010000000
VERSION     DASRYE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161365"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFF98_04020"
     CDS             complement(<1..272)
                     /locus_tag="VFF98_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU62828.1"
                     /translation="MKTQVAIIGAGPSGLLLGHLLKAEGIDCVVLERQTGDYVLGRIR
                     AGVLENITVNLMERLGLDQRLKAEGLIEEGFNLADGERLIRIDVANL"
     gene            complement(447..2084)
                     /locus_tag="VFF98_04025"
     CDS             complement(447..2084)
                     /locus_tag="VFF98_04025"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZU62829.1"
                     /translation="MPPARHALAHALLNNPLTNKGTAFSEQERDEYGLHGLLPPHVGT
                     LSGQIDRRRKVLEGMDSDFHRYAFLRELQDSNEILFHALVQSDLPRMLPLVYTPTVGE
                     GCERFSEIWRRPRGLFISYPNRHLIDDILANPALDRVKVIVVSDGERILGLGDQGAGG
                     MGIPIGKLALYTACAGIHPAEVLPILLDVGTDNPVRRDDPIYIGWQAPRVRGAEYDAF
                     LEDFVAAVERRFPGVLLQWEDFAGKNAYDLLGRYRHRLLSFNDDIQGTAATAVGTLLA
                     AVRKTGVPLAEQRVVLFGAGAAGSGIAEMLVRAIMAEGLSEAEARGRVWALDRDGLIH
                     AGMNNLPPQQVNLARPSAEIEAWPRRSSGLIGLHEVIAHIRPTVLIGASGQKNAFDEG
                     VIRLMAAGVDRPVIFPLSNPVSRAEAHPADILLWSEGRAIVGTGSPFGTPGVTQVNNV
                     YVFPGVGLGALVTAAREITDNMFMAAARRLGTLVDFEHERSSGGLLPAITDLRHVALE
                     VALAVVEQAQEDGVCTVAPEQLTREAIAQRMWAPLYE"
     gene            complement(2236..3906)
                     /locus_tag="VFF98_04030"
     CDS             complement(2236..3906)
                     /locus_tag="VFF98_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU62830.1"
                     /translation="MIGEDISIAEALPGDLLHPPQGEAGTGELHRTLDWKGAFWASSG
                     VPAGVLLTMGGIAATIGQPSWVVWIVSILMGFVQSFVYAEIAGLYPHKSGGASVYGAA
                     AWLPYSKFVAPISVWCNWLAWSPVLTLGTSLGAGYVMASLFAPTAAINNWHITLLDLG
                     FIRSGLTLRVNAVSLVGTAFLLITFALQHQGAARAAYAQKLLGLACLAPLVLVGLVPL
                     ITGDLPQAHLFPLLPLLRDAAGHAHFGTWNAYGLTLLMGAMFGAGWSTYGFETAVCYT
                     REFRDPARDTPRAIIASGLLCLVIFALVPLAFQASLGLSGMLDPAIYDGSGVALALAH
                     IVGGGAVVANVFIAMLVLALLLIVMTSMMGSSRTLYQASVDGWLPRYLSRVNRHGAPT
                     AAMWTDLGFNLVLLLASDYFAVLMISNVCYLLFNFLNLQSGWIHRIDRKGWDRPFRCP
                     TWLLALGTVFGFVNMAFMGAGADVWGQGTLRNGLISAALIVPVFLFRHYVQDKGRFPH
                     ALAEDFAEAGPGGAIATPRRAGVLPWAALAACIAVVALAHHFAQLIPF"
     gene            complement(3903..5570)
                     /gene="betA"
                     /locus_tag="VFF98_04035"
     CDS             complement(3903..5570)
                     /gene="betA"
                     /locus_tag="VFF98_04035"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HZU62831.1"
                     /translation="MPHTDESFDYIIVGAGSAGCVLANRLSASGQHKVLLLEFGGSDR
                     SIYIQMPAALAYPMNTKRWNWHYESEPEAHLNNRRLHCPRGKGLGGSSSINGLVYVRG
                     NPLDFERWEQEGARGWGYAQVLPYFKRAETRKEGGNAYRGGEGPLSTTYGTLENPLNQ
                     AWLDAAREAGYALTEDYNGFRQEGFGRMDMTVRDGERCSSAKAHLYDAVKRPNLKVIQ
                     QALATRVLFEGRRAVGVEYERGGQLHVAKAAREVILSGGPINTPQLLKLSGIGPAEEL
                     ARHGIPVLADRAGVGGNLQDHLEFYFQMACTQPITLFGHANLLGKAKVGLEWLLLKKG
                     LGASNHFETCGFIRSRAGIRYPDIQYHFFPMAINYDGSSLATEHGFQAHVGPMRSKSR
                     GTVTLRSADPREKPVIRFNYMSHPDDWAEMRACVRLTREIFQQPAFAPWRGREIQPGA
                     NCTSDEDIDAFIADKVESALHPSCTARMGSPANPLAVVDPECRVIGVEGLRVVDSSIM
                     PSITTGNLNAPTIMIGEKAADHILGKGLLPPANEPFYVAPDWEVSQR"
     gene            complement(5557..6963)
                     /gene="betB"
                     /locus_tag="VFF98_04040"
     CDS             complement(5557..6963)
                     /gene="betB"
                     /locus_tag="VFF98_04040"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HZU62832.1"
                     /translation="MTHFDTINPATGDILASIRIDGAAEIEAAVARARVAQKEWARLT
                     GTERGRILRRAVDILRARNDELAILEAKDTGKPIQETSAVDILSGADAIEYFANVAAA
                     IAGEHVDLGPAAFGYTRREPLGITAGIGAWNYPIQIACWKSAPALACGNAMIFKPSEL
                     TPLSAVELEKIYIEAGCPEGLFQVVQGFAETGRLLTRHPAIAKISLTGSVPTGRKLMA
                     DAAETLKQVTLELGGKSPLIVFADADLDNAVSGAMLGNFYSAGEVCSNGTRVFVERSV
                     LPAFLEKLKARTEAMILGDPLDPATQMGALINTAHMDKVLGYIEKGKAEGATLVTGGA
                     RVTEGACASGAFVAPTIFADCTDAMTIVREEIFGPVMTVLAFDSEEEVVARANDTEFG
                     LAGAVFTADLTRAHRVIARLEAGTCWINTYNITPIELPFGGYKQSGLGRENSRLAIEH
                     YTRVKSVYVAMEPIDAPY"
     gene            complement(7057..9399)
                     /locus_tag="VFF98_04045"
     CDS             complement(7057..9399)
                     /locus_tag="VFF98_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62833.1"
                     /translation="MAITPAQADEAVPASNPASAPGDIVVTAQHREQKLNDVGIAVSV
                     LGAQQIKNLNITSATDIVRAIPNLHFNAYGSSQVVFNIRGVSQNDYGDQQEPPVAVYQ
                     DDSYSSSITTASFPIFDLARTEVLRGPQGTLFGRNATGGAVQFISAQPTDHTDGYLSA
                     TYGSYNQRIIEGALSGRISDTLTARIAGQYDRDDGYIRDITPGQPNRGADNHWALRGI
                     LKWEPTDDFKARLTVRYAEADHERQAGNYTLSPACPNAQGQGEYLAVGQACSFWGTTN
                     GGTANGYVNPSISPSQGGNPWTTAGTGDSYVNRQQLGISLRLEGKIGAFDVTSITDYQ
                     HLKKFYTEQGDGDPEQVYTGAGSASSEPTACISGSSTLCYPSGTVFNQDAHTSQFTQE
                     LRAATNFGHQYLTFGAFGMVLDGKYSAKYATPFDGYDPQMYFSQRTESFAFFAQDEWK
                     ISDQWKLIGGLRYWHDHKVGNYSGCEYYTGLCVNWGGSGIGYVTGYGSAYSTEGITAT
                     VADTRPSFSGVTARAEVDYKPARDTLIYASYNRGSKSGGFTFSTGTPYASAIVDTLNN
                     IAYKPETLDDYEVGLKTKIGRVSQLNLAAFYYDYHNYQAYMQVGYTQVVRNLPATVKG
                     IEAEFTTRPIRGLTFQANGALQESLVKGVLLPDGITVVNHAMPQAPGFSGSALIRYEF
                     ALLGGTASGQFDASYTGKTCFTVMCAPVEEEPAYHVENLRIGFTPAGGKVDAAFFINN
                     LLNRAYRSYAFDGSQYWGDALSVYAKPRTWGVTLTYHLGR"
     gene            complement(9458..9658)
                     /locus_tag="VFF98_04050"
     CDS             complement(9458..9658)
                     /locus_tag="VFF98_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62834.1"
                     /translation="MAAQAAEFRHFLQKMGGVAGFVLRGLSLQRGPSSGTLPAEEFEE
                     FDEGRLNDSGESHEEHIHRENP"
     gene            complement(9794..>9932)
                     /locus_tag="VFF98_04055"
     CDS             complement(9794..>9932)
                     /locus_tag="VFF98_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZU62835.1"
                     /translation="HLAGMKDSKTIIAINKDEDAPIFQVADIGLVADLFSAVPEIAAK
                     A"
BASE COUNT         1735 a         3215 c         3332 g         1650 t
ORIGIN      
        1 agattggcca catcgatgcg gatcagccgc tcgccatcgg ccaggttgaa gccttcctcg
       61 atcaggccct cggccttcag gcgctggtcg aggccgaggc gctccatcag gttgacggtg
      121 atgttttcca gcacgccggc gcggatgcgg cccagcacat aatcgccggt ctgccgctcc
      181 agcaccacgc aatcgatgcc ctcggccttc agcagatggc ccagcagcag gcccgagggg
      241 cccgcgccaa tgatcgcgac ttgggttttc atggttttct ctccccacgg gatggtgtgg
      301 gccagctttc gcgccggccg cgctgggggg aggaaatgac atgcaaggct ggggggatga
      361 aggcaagcct cgtgcaaagg cttggacttt tcgtgcaggg gcggaaaagg gggcgcgggc
      421 cttgccccgc ccccttccct catcgcttat tcgtaaagcg gcgcccacat ccgctgggcg
      481 atcgcctcac gcgtcagctg ctcgggcgcg acggtgcaca cgccatcttc ctgcgcctgt
      541 tccaccaccg ccagcgccac ttccagcgcc acatggcgca gatcggtgat cgccggcagc
      601 aggccgccgc tcgaccgttc atgctcgaaa tcgaccagcg tgcccaggcg cctcgcggcc
      661 gccatgaaca tgttgtcggt gatctcgcgc gcggcggtga ccagcgcgcc aagccccacg
      721 cccgggaaga cgtagacatt gttcacctgc gtgacgcccg gcgtgccgaa cgggctgccg
      781 gtgccgacga tggcgcggcc ctcgctccac agcaggatgt cggccgggtg cgcctcggcg
      841 cgcgaaacgg ggttcgacag cgggaagatc accggacggt caacgcctgc cgccatcagc
      901 cgtatcacgc cctcgtcaaa cgcattcttc tggcccgagg cgccgatcag cacggtgggc
      961 ctgatgtggg cgatcacctc atgcaggccg atcaggccgc tggaacggcg cggccaggcc
     1021 tcgatctcgg cgctggggcg ggccaggttc acttgctgcg gcggcaggtt gttcatgccg
     1081 gcatggatca gcccgtcgcg gtccagcgcc cagacgcggc cgcgcgcctc tgcttccgaa
     1141 agcccctcgg ccatgatcgc gcgcaccagc atctcggcaa tgccgctgcc ggccgcgccc
     1201 gcgccaaaca gcaccacgcg ctgctcggcc agcggcacgc cggtcttgcg cacggcggcc
     1261 agcagggtgc cgacagcggt ggccgcggtg ccctggatgt cgtcgttgaa ggagagtagg
     1321 cggtggcgat agcggcccag caggtcatag gcgttcttgc cggcaaaatc ctcccactgg
     1381 agcagcacgc cggggaagcg gcgctccacc gcggcgacga aatcttccag gaaggcatca
     1441 tattcggcgc cccgcacgcg cggcgcctgc cagccgatat agatcggatc atcgcggcgc
     1501 acgggattgt cggtgccgac atcgagcagg atcggcagca cttcggccgg gtgaatgccg
     1561 gcgcaggcgg tgtagagcgc cagcttgccg atcgggatgc ccatgccgcc ggcgccctga
     1621 tcgcccaggc ccagaatgcg ctcgccatcg gagacgacga tcaccttcac ccgatccagc
     1681 gccggattgg cgaggatgtc gtcgatgagg tggcggttgg gataggagat gaacaggccg
     1741 cgcgggcggc gccagatttc cgaaaagcgc tcgcagccct cgcccacggt gggcgtgtag
     1801 accagcggca gcatgcgcgg cagatccgat tgcaccagcg cgtggaagag gatctcgttg
     1861 gaatcctgca gctcgcgcag gaaggcatag cggtggaaat cgctgtccat cccttccagc
     1921 accttgcggc ggcggtcgat ctggcccgac agcgtgccga catggggggg cagcaggccg
     1981 tggaggccgt attcgtcgcg ctcctgctcg gaaaaggcgg tgcccttgtt ggtcagcgga
     2041 ttgttgagca gggcatgggc gagcgcatgg cgggctggag gcacgggcga caggttcctt
     2101 gattggggcg gcgcgcgagg aacgcgccgt caaggtgggg ggaggagaga ctgagcccgt
     2161 aggccagcgg gcccgggaag gcaaggcagc ccgcgcgcct tgcttttccc gggccttttt
     2221 cccgcccggc gcccttcaga aagggatcag ctgtgcgaaa tgatgggcca gcgccaccac
     2281 cgcgatgcag gccgccagcg ccgcccaggg cagcacgccc gcccggcgcg gcgtggcgat
     2341 ggcgccgccc ggccccgctt cggcgaaatc ttccgccagg gcgtggggga agcggccctt
     2401 gtcctgcaca tagtggcgga agaggaacac cggtacgatc agcgccgcgc tgatcaggcc
     2461 attgcgcaag gtgccctgcc cccacacatc ggcgcccgcc cccatgaagg ccatgttgac
     2521 aaagccgaac accgtgccca gcgccagcag ccaggtgggg cagcggaagg ggcgatccca
     2581 gcccttgcga tcgatccggt ggatccagcc cgattgcagg ttgaggaaat tgaacaggag
     2641 gtagcacacg ttgctgatca tcagcaccgc gaaataatca gacgccagca gcaggaccag
     2701 gttgaagccc agatccgtcc acatggcggc ggtgggcgcg ccatggcggt tgacgcgcga
     2761 cagatagcgc ggcagccagc catccaccga ggcctgatag agcgtgcgcg aggagcccat
     2821 catcgaggtc atcacgatga gcagcagcgc cagcaccagc atggcgatga acacattggc
     2881 caccaccgcc ccgccgccca cgatatgcgc cagcgccagc gccacgcccg agccatcgta
     2941 gatcgccgga tcgagcatgc cgctgagccc cagcgaggcc tggaacgcca gcggcaccag
     3001 cgcgaagatg accaggcaca atagccccga ggcaatgatc gcgcgcggcg tgtccctggc
     3061 cggatcgcgg aattcacgcg tgtagcacac cgccgtctcg aagccgtagg tggaccagcc
     3121 ggcgccgaac atcgccccca tcagcagcgt caggccatag gcgttccacg tgccgaaatg
     3181 ggcgtggccg gcggcatcgc gcagcagcgg cagcagcggg aagagatgcg cctgcggcag
     3241 atcgccggtg atcagcggca ccagccccac cagcaccagc ggcgccaggc aggcaaggcc
     3301 cagcagcttt tgcgcatagg ccgcccgcgc cgccccctga tgctgcaagg caaaggtgat
     3361 gagcagaaag gccgtgccga ccagcgagac ggcattcacc cgcagggtca acccgctgcg
     3421 gatgaagccc agatcgagca gcgtgatgtg ccagttgttg atcgccgctg tgggggcaaa
     3481 caggctggcc atcacatagc ccgcgccaag gctggtgccg agcgtgagca ccggcgacca
     3541 ggccagccag ttgcaccaca ccgaaatggg cgcaacgaac ttgctgtagg gcagccaggc
     3601 cgccgcgcca tagacagaag ccccgcccga cttgtgcgga tagagcccgg cgatctcggc
     3661 atagacgaag ctttgcacga aacccatgag gatggagacg atccacacca cccagctggg
     3721 ctggccgatg gtggcggcaa tgccgcccat cgtcagcagc acgccggccg gcacgcccga
     3781 ggaggcccag aaggccccct tccagtcaag cgtgcggtgg agttcgcccg tgcccgcctc
     3841 gccctggggc gggtgcagca gatcgcccgg cagcgcctcg gcgatggaga tgtcctcgcc
     3901 gatcatcgct ggctcacctc ccagtcgggc gccacataga agggctcgtt ggcgggcggc
     3961 agcaggccct tgccaaggat atgatcggcc gctttctcgc cgatcatgat ggtgggggca
     4021 ttgaggttgc cagtggtgat cgagggcatg atcgagctgt cgaccacccg gaggccttcc
     4081 acgccgatca cccggcattc cggatcgacc accgccagcg gattggccgg gctgcccatg
     4141 cgcgccgtgc aggaaggatg cagcgcgctt tccaccttgt cggcgatgaa ggcgtcgatg
     4201 tcctcgtcgc tggtgcagtt ggcgccgggc tggatctcgc ggccgcgcca gggggcaaag
     4261 gccggctgct ggaagatctc gcgcgtcagc cgcacgcagg cgcgcatttc tgcccagtcg
     4321 tccgggtggc tcatgtagtt gaagcggatc accggcttct cgcgcgggtc ggccgaacgc
     4381 agggtgacgg tgccgcggct cttggagcgc atcggcccga catgggcctg gaagccatgt
     4441 tcggtggcca gcgacgagcc atcgtaattg atcgccatcg ggaagaagtg atactggata
     4501 tcgggatagc gaatgcccgc ccggctgcgg atgaagccgc aggtttcaaa gtggttggaa
     4561 gcgcccagcc ccttcttcag caacagccat tcgaggccca ccttcgcctt gcccagcagg
     4621 ttggcatggc caaacagggt gatcggctgg gtgcaggcca tctggaaata gaattccagg
     4681 tgatcctgca gattgccgcc cacgcccgcc cggtctgcca gcacggggat gccgtggcgg
     4741 gccagttcct cggccgggcc aatgcccgac agcttgagca gctgcggcgt gttgatcggc
     4801 ccgcccgaaa ggatcacctc gcgcgcggcc ttggccacat gcagctggcc gccgcgctca
     4861 tattccacgc ccaccgcccg gcgcccctcg aacagcacgc gcgtggccag cgcctgctgg
     4921 atgaccttga ggttgggccg cttcaccgca tcatagagat gggccttggc cgaggagcag
     4981 cgttcgccat cgcgcacggt catgtccatg cggccgaagc cctcctggcg gaagccgtta
     5041 taatcttccg tcagcgcata gccggcctca cgcgcggcat cgagccaggc ctggttgagc
     5101 gggttttcca gcgtgccata ggtggtggac aagggcccct cgccgccgcg ataggcattg
     5161 ccgccttcct tgcgggtttc cgcccgcttg aaatagggca gcacctgcgc atagccccag
     5221 ccgcgcgcgc cttcctgctc ccacctttcg aaatccagcg ggttgccgcg cacatagacc
     5281 agcccgttga tcgaggagga accgcccagc cccttgccgc gcgggcaatg cagccggcgg
     5341 ttgttgagat gggcttcggg ctcgctctcg tagtgccagt tccaccgctt ggtgttcatc
     5401 ggataggcaa gggcggcggg catctggatg tagatcgagc ggtccgagcc gccgaattcg
     5461 agcagcagca ccttgtgctg gccgctggcc gaaaggcggt tggccagcac gcagccggcc
     5521 gatcctgccc ccacgatgat atagtcgaag gactcgtcag tatggggcat cgatgggctc
     5581 catggcgaca tagacgcttt tcacgcgcgt gtagtgctcg atggccaggc ggctgttctc
     5641 gcggccaagg ccggattgct tgtagccgcc aaagggcagt tcgatcggcg tgatgttata
     5701 ggtgttgatc cagcaggtgc ccgcttccag ccgggcgatc acccggtggg cgcgggtgag
     5761 atccgccgtg aagacagcgc cggccaggcc gaattcggtg tcattcgcgc gggccaccac
     5821 ttcctcctcg ctgtcgaagg caaggacggt catcaccggg ccgaagatct cctcgcgcac
     5881 gatggtcatc gcatcggtgc aatcggcaaa gatggtgggg gccacaaagg cgccgctggc
     5941 gcaggcgccc tctgtcaccc gcgcgccgcc ggtaacgagc gttgcccctt cggccttgcc
     6001 cttctcgata tagcccagca ccttgtccat atgggcggtg ttgatcagcg cgcccatctg
     6061 cgtggccgga tccagcggat cgcccaggat catcgcctcg gtgcgcgcct tcagcttttc
     6121 caggaaggcc ggcagcaccg aacgttcgac aaagacgcgg gtgccgttgg agcacacctc
     6181 gccggccgaa tagaaattgc ccagcatcgc gccggaaacg gcattgtcga gatcggcatc
     6241 ggcaaagacg atgagcgggc tcttgccgcc cagctccagc gtcacctgct tcagcgtctc
     6301 ggcggcatcg gccatcagct tgcggccggt gggcacggaa cctgtgagcg agatcttggc
     6361 gatcgccgga tgacgggtga gcaggcggcc ggtttcggca aagccctgca ccacctggaa
     6421 caggccttcg gggcagcccg cctcgatata gatcttctcc agctccaccg cgctcagcgg
     6481 ggtcagctcg gacggcttga agatcatcgc attgccgcag gccagcgccg gcgccgattt
     6541 ccagcaggcg atctggatgg gatagttcca cgcgccgatg ccggcggtga tgcccagcgg
     6601 ctcgcgccgg gtgtagccaa aggcggccgg gcccaggtcc acatgctcgc cggcgatggc
     6661 ggcggccaca ttggcgaaat attcgatggc atcggcgccc gacaggatat cgaccgcgct
     6721 ggtctcctgg atcggcttgc cggtgtcctt ggcttccagg atcgccagct cgtcgttgcg
     6781 ggcgcgcagg atatccaccg cccggcgcag gatgcggccg cgctcggtgc cggtgaggcg
     6841 cgcccattcc ttctgggcca cgcgggcccg ggccacggcg gcctcgatct cggccgcgcc
     6901 atcgatgcgg atggaggcca gaatgtcgcc ggtggcggga ttgatggtgt cgaaatgggt
     6961 catgggtatg gctgctcgca aaaaggaaga gagcggccct gtgggcggcg cggggcaaga
     7021 cctgcccgcc cgcgccgccc gccaggaccg atgcgcttac ctgcccagat ggtaggtgag
     7081 agtcaccccc caggtgcgcg gcttggcgta aacgctcagc gcatcgcccc aatactgcga
     7141 cccgtcgaac gcatagctgc gataggcgcg attgagcagg ttgttgatga agaaggcggc
     7201 atccaccttg ccccctgccg gcgtgaagcc gatgcgcagg ttctccacgt gataggccgg
     7261 ctcttcctcc accggcgcgc acatgacggt gaagcaggtc ttgccggtgt agctggcatc
     7321 gaactgaccc gaggccgtgc cgcccagcag cgcgaactca taacggatca gcgcgctgcc
     7381 cgagaagccc ggcgcctgcg gcatggcatg gttcaccacg gtgatgccat ccggcagcag
     7441 cacgcccttc accaggcttt cctgcagggc gccattggcc tggaaggtga ggccgcggat
     7501 cgggcgggta gtgaattcgg cctcgatgcc cttcaccgtg gccggcaggt tgcgcaccac
     7561 ctgggtgtag cccacctgca tataggcctg ataattgtgg taatcgtaat agaaggcggc
     7621 aaggttcagc tggctgaccc ggccgatctt cgtcttcagg cccacttcat agtcgtcgag
     7681 cgtctcgggc ttgtaggcga tgttgttcag cgtatcgacg atggcgctgg catagggcgt
     7741 gccggtgctg aaggtgaagc cgcccgactt ggagccgcgg ttgtagctgg catagatcag
     7801 cgtgtcgcgc gcgggcttgt agtccacctc ggcgcgggcg gtaacgccgc tgaagctggg
     7861 gcgggtgtcg gccaccgtgg ccgtgatgcc ctcggtggaa taggccgagc catagccagt
     7921 gacatagcca atgccgctgc cgccccagtt gacgcacagg ccggtgtaat attcgcagcc
     7981 cgaataattg cccaccttgt gatcgtgcca gtaacgcagg ccgccaatca gcttccactg
     8041 atcggagatc ttccattcat cctgcgcgaa gaaagcaaag ctttcggtgc gctgggagaa
     8101 atacatctgc ggatcatagc catcaaaggg cgtggcatat ttggccgaat atttgccatc
     8161 cagcaccatg ccaaaggcgc cgaaggtgag gtattggtgg ccaaaattgg tggccgcgcg
     8221 cagttcctgg gtgaactggc tggtgtgcgc atcctggttg aacaccgtgc ccgagggata
     8281 gcacagcgtg ctggagcccg agatacaggc ggtcggctcg ctggaagcgc tgccggcgcc
     8341 ggtgtagacc tgctcgggat cgccatcgcc ctgttcggtg tagaacttct tcaggtgctg
     8401 gtaatcggtg atcgaggtaa cgtcgaaggc gccgatcttg ccctcaagcc gcaggctgat
     8461 gcccagctgc tggcggttca cgtaggaatc gccggtgccc gccgtcgtcc agggattgcc
     8521 cccctgcgag gggctgatcg aggggttcac atagccattg gcggtgccgc cattggtggt
     8581 gccccagaag gagcaggcct ggcccacggc cagatattca ccttggccct gcgcattggg
     8641 gcaggccggc gacagcgtgt agttgccggc ctggcgctcg tggtcggcct cggcatagcg
     8701 cacggtgagg cgggccttga aatcgtcggt cggctcccac ttcaggatgc cgcgcagggc
     8761 ccagtggttg tcggcgccgc ggttgggctg gccgggggtg atgtcgcgga tataaccatc
     8821 gtcgcggtcg tactggccgg cgatgcgcgc ggtcagcgtg tcgctgatcc ggcccgacag
     8881 cgcgccctcg atgatgcgct ggttatagct gccataggtg gccgagagat agccgtcggt
     8941 gtggtcggtc ggctgggccg agatgaactg caccgcgccg cccgtggcat tgcggccaaa
     9001 cagcgtgccc tgcggcccgc gcagcacttc ggtgcgggca aggtcgaaga tcgggaagct
     9061 cgccgtggtg atcgacgagg aatagctgtc gtcctggtag acggcaaccg gcggctcctg
     9121 ctggtcgcca taatcgttct gcgacacgcc gcggatgttg aacaccacct ggctggagcc
     9181 ataggcgttg aaatgcaggt tggggatggc ccgcacgata tccgtggcgg atgtgatgtt
     9241 gaggttcttg atctgctgcg cgcccagcac gctgacggca atgccgacat cgttgagctt
     9301 ctgctcgcgg tgctgcgctg tcaccacgat atcgcccggc gcgctggccg gattgcttgc
     9361 cggcacggcc tcgtcggcct gcgcgggtgt aatcgccacg ccacccagcc caccaaccat
     9421 cgcggccagc gcgacaccaa ggcggacatt atggcggcta cggattttcc ctgtggatat
     9481 gctcttcatg gctttctcct gaatcgttca gcctgccctc atcgaactcc tcgaactcct
     9541 ccgccggcaa ggtgcccgag gaggggccgc gctgcaagga aaggccgcgc agaacgaaac
     9601 cggccacacc gcccattttc tgcaaaaaat ggcggaattc agcggcctga gcggccattc
     9661 accacattac ctcaacttat actcttcatt agatttcaaa cagacacccc atgccgcaat
     9721 gcacacaaac aaaaccgccg ccagcaccga aaagggcgct ggcggcggtc tgtctcgcaa
     9781 aaggaaaagc ccctcaggcc ttggcggcga tttccggcac ggctgagaag aggtcggcga
     9841 cgaggccgat gtctgcgacc tggaagatgg gtgcgtcttc gtccttgttg atggcgatga
     9901 tggttttgct gtccttcatg ccggcgaggt gc
//
LOCUS       DASRYE010000072         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_145484,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000072 DASRYE010000000
VERSION     DASRYE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145484"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..86)
                     /locus_tag="VFF98_04060"
     tRNA            complement(10..86)
                     /locus_tag="VFF98_04060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Arg,seq:acg)
     gene            complement(145..221)
                     /locus_tag="VFF98_04065"
     tRNA            complement(145..221)
                     /locus_tag="VFF98_04065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Arg,seq:acg)
     gene            complement(288..728)
                     /locus_tag="VFF98_04070"
     CDS             complement(288..728)
                     /locus_tag="VFF98_04070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HZU62836.1"
                     /translation="MSSRVSPLLVVAVALLRAEGQVLMQQRPPGKAHAGLWEFPGGKL
                     EAGESAEIAACREIAEELGVVLRPADLVPVGFASNGGGLVILLYACRAWEGQPAALEG
                     GDIGWFALVDVAGLAMPPLDYPLAEALLRAAPRVCGEAGHPCAR"
     gene            804..1010
                     /locus_tag="VFF98_04075"
     CDS             804..1010
                     /locus_tag="VFF98_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZU62837.1"
                     /translation="MAVLSFLVRLLKDNKALSAVEYGLICSLIVLAMIAGLQNFASGV
                     INMWNNVATQVSTAVAQANGNAAS"
     gene            1278..1451
                     /locus_tag="VFF98_04080"
     CDS             1278..1451
                     /locus_tag="VFF98_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZU62838.1"
                     /translation="MKFINKFFRNEAGATAIEYGLIAALIAVAAISAMSGLGTQLKST
                     FNTTSSAMAGAAS"
     gene            complement(1597..2544)
                     /locus_tag="VFF98_04085"
     CDS             complement(1597..2544)
                     /locus_tag="VFF98_04085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZU62839.1"
                     /translation="MLSYLEFEKPVAALDARIAELRATAEQGDLDISAEIGRLERKSA
                     EMLESTYKALSPWQKTQVARHPQRPHFLDYVEAIFTDFVPLGGDRVYGEDQAIIGGFA
                     RLGERRVMLIGHEKGHDTATRLKHNFGMGKPEGYRKAIRLMELAGRFGLPVVTLVDTS
                     GAFPGVEAEERGQAEAIARATEACLGLPVPMVAVIVGEGGSGGAVALASAERVLMLEH
                     AVYAVISPEGCASILWRTAERAADAAEAMKVTAQDLLGLKVIDRIVPEPVGGAHRNPA
                     GMARALSKAIDEELTQLSTLSARALQAQREERFLRMGEL"
     gene            complement(2611..3495)
                     /locus_tag="VFF98_04090"
     CDS             complement(2611..3495)
                     /locus_tag="VFF98_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HZU62840.1"
                     /translation="MSRPILAFLAMLSAERGAAANTVEAYGRDLNAAEEVLGDLLEAD
                     ATAIAGLGEAWADMAPASLARRCSALRQFYHFMQEEGWRADDPSPALPRVSPRRPLPR
                     LVGHGEVAALFACAEEEAAGDAPAPLRLLACLELLYGSGLRVSELVGLPVAAVPRDAP
                     FLMLRGKGGVERMVPVSTRARQVLGRWLAVRGGMARADLPFLFPSRGGSGHFTRVRLF
                     QLLRELALRAGVAPERISPHVLRHAFATHLLEGGADLRVLQTLLGHADIATTQIYTHV
                     EASRLVALVNARHPLAER"
     gene            complement(3500..>4829)
                     /locus_tag="VFF98_04095"
     CDS             complement(3500..>4829)
                     /locus_tag="VFF98_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62841.1"
                     /translation="RLDAPEGLDPADFATLRLALLMRMGEAEAARALAQDVDPDNATP
                     AFAQAALEAYVATEDFTGFCPLLEAVGVEAGGKDPRWQVMQGMCDVFAGNGSEGFARL
                     DDPATAGNLAPGDMLLAQKYAGAAGAGRRSITMDWGKTTDMTPWRYGLALATGLTVPA
                     TLMKDAGPRYDYVTASAPMVGLGLRADAADASAGAGILSAAAMVDLYSQIYANDDVTG
                     PPADRALLLRDAYVADTPAARIKAMQGLWEGAADPRGRYARQVLTAYAAARLPASADN
                     ADAAGDLIASMLAAGLDANAQRWAGAVEKGSLGWGLIALAAPGGGQVASADIDSFHNA
                     DKSANARKSAFLVAGLAGLGRIDDATRNNWTHQLNAGLDTRSHWIELIDGAAAVHNQA
                     LVALLAGLGMQGDSWAQMSPRYLYHIVGALSRVGLAAEARMIAAEAVARG"
BASE COUNT          835 a         1737 c         1507 g          750 t
ORIGIN      
        1 ggtggaaaat ggcgcacccg gaacgattcg aacgtccgac cctcagattc gtagtctgat
       61 gctctatcca gctgagctac gggtgcgcgt cttgcactat ggcagggcca aggccccttc
      121 ccttatttcc accgtggtgg aaaatggcgc acccggaacg attcgaacgt ccgaccctca
      181 gattcgtagt ctgatgctct atccagctga gctacgggtg cgtctgggag acgcgcctct
      241 aggaggagtt cgcagggttg gcaagcacaa aatgacacaa tttctgatta tcttgcgcaa
      301 gggtgcccgg cctccccgca aacccttggc gcggcgcgca gcagcgcctc tgccagcgga
      361 taatccagcg gcggcattgc cagccccgcc acgtcgacca gcgcgaacca gccgatatcg
      421 cccccttcca gcgccgccgg ctgcccctcc cacgcccggc aggcatagag caggataacc
      481 agcccgccgc cattgctggc aaagcccacc ggcaccagat cggccgggcg cagcaccacg
      541 cccagctcct cggcgatttc gcggcaggcg gcgatttcgg cagattcgcc cgcttccagc
      601 ttgccaccgg gaaattccca gaggccggca tgggccttgc cgggcgggcg ctgctgcatc
      661 agcacctggc cttctgccct gagcaaagcc acggccacaa ccaacagagg tgaaacgcga
      721 gaggacatgc acgtcgattt cgtgaaaatt tttattaaca gccttgggct atatgccgca
      781 aaattgctct tgggtgagat ttgatggctg tcctttcctt cctggtccgc ctgctgaaag
      841 acaataaggc gctctcggca gtggaatatg ggctgatctg cagcctgatc gtgctggcga
      901 tgatcgccgg cctgcagaat ttcgccagtg gcgtgatcaa catgtggaac aatgtcgcaa
      961 cgcaggtgtc caccgccgtg gcccaggcca acggcaacgc cgccagctga tcgcgccccc
     1021 agcgcgccaa cagcccacac cttggttaac agcctgcgcc ccgcgccggc ggcccgcccg
     1081 cttgcagcgg cagcgcccac gcgcgcgcgc cagcccatcc agactgataa tccggaaaaa
     1141 tacgcaaaat tgcttttacc ccgctcaggc aaaaatcctg ctgacggttt gttaagaact
     1201 tttgcctaga agggcttcac cgcccggtga gacgggtaac aagcggggcg ggcagatcga
     1261 ttttttggag accggacatg aagttcatca acaagttctt ccgcaacgaa gctggcgcca
     1321 ccgcgatcga atacggcctg attgccgccc tcatcgccgt tgccgcgatc tcggccatga
     1381 gcggcctggg cacccagctg aagagcacct tcaacaccac ctcgtcggcc atggctggcg
     1441 ccgcttcgta agaagcgcgc ggctgaacaa gccgggtaga aaaagggcgg cgggacattt
     1501 cccgtcgcct ttttctgtgc ctcctgtgcg ggcgcaaaaa aggctgcgaa gggggcaacc
     1561 cttgcgcagc cttttgaatg ccggtgctgt tggcgcttaa agctcgccca tccgcaggaa
     1621 gcgttcctcg cgctgggcct gcagggcgcg ggctgacagc gtactgagct gggtcagctc
     1681 ctcgtcgatc gccttggaca gcgcacgcgc catgcccgcc gggttgcgat gggcgccgcc
     1741 caccggctcg ggcacgatgc ggtcaatcac tttcagaccc agcaggtcct gcgccgtcac
     1801 cttcatcgcc tcggcggcat cggcggcgcg ctcggccgtg cgccacagaa tcgaggcgca
     1861 gccttccggg ctgatcacgg cataaacggc atgttccagc atcagcacgc gctcggcact
     1921 ggccagcgcc accgcgccgc ccgaaccacc ctcgccaacg atcaccgcca ccatcggcac
     1981 cggcagacca aggcaggcct cggtggcgcg ggcaatcgcc tcggcctggc cgcgctcttc
     2041 ggcttccacg ccgggaaacg cgcccgatgt atccaccagc gtcaccaccg gcaggccaaa
     2101 gcgcccggcc agttccatca ggcggatcgc cttgcgatag ccctcgggct tgcccatgcc
     2161 gaaattatgc ttgaggcggg tggcggtatc atggcccttt tcgtggccga tcagcatcac
     2221 ccggcgctcg cccagccggg caaagccgcc gatgatcgcc tgatcctcgc catagacgcg
     2281 atcgcccccc agcggcacga aatcggtgaa gatcgcctcc acataatcga ggaaatgcgg
     2341 ccgctgcggg tggcgggcca cctgcgtctt ctgccagggg ctcagcgcct tgtaggtgct
     2401 ttccagcatt tcggcgctct tgcgctccag ccggccgatc tcggcggaaa tgtcgagatc
     2461 gccctgttcg gcggtggcgc gcagctcggc gatgcgcgca tcaagggcgg ccaccggctt
     2521 ttcgaattcg agataggaca gcatgccccc cgcctaatcg cccctgtccc tcgcggcaag
     2581 tgcgtaaacg cttcgggcgg tttaagcggc ttaacgttcg gccagcgggt ggcgggcgtt
     2641 caccagcgcc accaggcggc ttgcttccac atgggtgtag atctgggtgg tggcgatatc
     2701 ggcatggccc agcagcgttt gcagcacgcg cagatccgcg ccgccctcca gcagatgggt
     2761 ggcaaaggca tggcgcagca catgcgggct gattcgctct ggcgcaacgc cggcgcgcag
     2821 ggccagttcg cgcagcagct ggaacaggcg cacacgggtg aaatgcccgc tgcccccccg
     2881 cgaggggaag agaaagggca aatccgcccg cgccatgccc ccacgcaccg ccagccagcg
     2941 gcccagcacc tgccgcgccc gggtgctcac cggcaccatc cgttccaccc cgcccttgcc
     3001 gcgcagcatc aggaacggcg catcgcgcgg caccgcggcc accggcaggc caaccagctc
     3061 gctgacccgg aggcccgagc catagagcag ctcaaggcag gccagcagcc gcagcggcgc
     3121 cggcgcatcg cctgccgcct cttcctcggc gcaggcaaac agcgcggcca cctcgccatg
     3181 gcccaccagc cgcggcagcg ggcggcgggg cgacacccgc ggcagcgccg gcgaaggatc
     3241 atccgcccgc cagccctcct cctgcatgaa atggtagaac tggcgcagcg ccgaacagcg
     3301 ccgggcaagg ctggccggcg ccatatcggc ccaggcctcg cccagcccgg caatcgcagt
     3361 ggcatcggcc tccagcaggt cgcccagcac ttcctcggcc gcattcaggt cgcggccata
     3421 ggcctccacc gtgttcgcag ccgcgccccg ctcggccgag agcatggcga ggaaggcaag
     3481 gatcggccgc gaaatggcgc tatccccgcg ccacggcttc ggcagcgatc atccgcgcct
     3541 cggccgccag ccccacgcgg ctgagcgcgc cgacgatgtg gtagagatag cgcgggctca
     3601 tctgcgccca gctgtcgccc tgcatgccca gccccgccag cagcgccacc agcgcctggt
     3661 tgtgcacggc cgccgcgcca tcgatcagtt cgatccagtg gctgcgcgtg tccagcccgg
     3721 cgttcagctg gtgagtccag ttgttgcggg tggcatcatc gatgcgcccc agcccggcaa
     3781 ggcccgccac cagaaaggcg cttttgcggg cattggcgct tttatcggca ttgtggaagc
     3841 tgtcgatatc ggcgctggcc acctgaccgc cgccgggcgc tgccagcgcg atcagccccc
     3901 agcccagcga gcctttctcc accgcgcccg cccagcgctg cgcattggca tccagccctg
     3961 ccgccagcat cgaggcgata agatcgcccg ccgcatcggc attgtcggcg ctggccggca
     4021 ggcgggcggc ggcataggcg gtcaacacct gccgcgcata gcgcccgcgc ggatcggccg
     4081 cgccctccca caggccctgc atcgccttga tgcgcgcggc cggcgtatcg gccacatagg
     4141 catcgcgcag cagcagggcg cggtcggccg gcggcccggt cacgtcatca ttggcgtaga
     4201 tctggctgta gagatccacc atcgccgcgg ccgagagaat gcccgccccc gcgctggcat
     4261 cggccgcatc ggcgcgcagg ccaaggccca ccatcggcgc gctcgccgtc acatagtcgt
     4321 agcgcggccc ggcatccttc atcagcgtgg ccggcaccgt cagcccggtt gccagcgcca
     4381 ggccatagcg ccagggggtc atgtcggtgg tcttgcccca gtccatggtg atggagcggc
     4441 ggccagcgcc ggcggcgccg gcatatttct gggccagcag catgtcgccg ggggccaggt
     4501 tgccggccgt tgccggatca tccagcctgg caaagccttc cgaaccattg ccggcaaaca
     4561 catcgcacat gccctgcatc acctgccagc gcggatcctt gccgcccgcc tccacgccca
     4621 ccgcttccag cagcgggcaa aagccggtga aatcctcggt ggcgacatag gcttccagcg
     4681 ccgcctgggc aaaggcgggc gtggcattgt cagggtccac atcctgggcc agcgcgcggg
     4741 cggcctcggc ctcgcccatg cgcatcagca gggccaggcg cagcgtggcg aaatcggccg
     4801 gatccagccc ctccggcgca tccagccgc
//
LOCUS       DASRYE010000073         8448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_20042,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000073 DASRYE010000000
VERSION     DASRYE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20042"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFF98_04100"
     CDS             <1..326
                     /locus_tag="VFF98_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HZU62842.1"
                     /translation="AYTPMSAGSIDPRWADYAAPEHVAPVAGWLCSDRCRTSGGIYHT
                     GAGHVRRVQLLEGPVHRLADGDIASVMQGIAALPEWSSSFASGAQILPAIAAAATIGA
                     SAHDD"
     gene            316..1272
                     /locus_tag="VFF98_04105"
     CDS             316..1272
                     /locus_tag="VFF98_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU62843.1"
                     /translation="MTIDFTGQVAIVTGAGGGMGRAYALELARRGAAVLVNDYGGSVL
                     GQQDGSPAPAEAVAAEIRDLGGRARACPLAVGSADHARAIVASALEAFGRLDILVNNA
                     GTALAGPFTAHDDAAVERHYAVNLVGGHHLMRAAWPHMAGQRYGRVLNVSSNGALGVG
                     GNAPYAAAKAGMLGLTLDAAIEGRPLGILVNAVMPTSHSRMIEQIPDPATVAWFRTHL
                     HTGQVAAAMAWFLSRACTESGRILLAGGGRLSSLVFAETGEIFTPALAAEDVAARIAE
                     VTDERVLSPVHSQAEAGARYFRHLPWTGESAADFGPDVPRGA"
     gene            complement(1296..2114)
                     /locus_tag="VFF98_04110"
     CDS             complement(1296..2114)
                     /locus_tag="VFF98_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU62844.1"
                     /translation="MPEPSRYLDRLSGKVALVTGAGAEAGEIGIGRAIALLLAREGAG
                     VLCADLDGARAEATAAMIRAGGGQALAMAGDVARPADCAALVEGCVAGLGRIDVLVNN
                     VGIAVPTSLADITLELWNATLAANLTSAMLMSKYAVPHMAGQGGGAIVNISSLAGMRA
                     MGAIAYGPSKAAMAQLSREIGVLHGRQGIRVNTVAPGHVMTPHAMAKVPAEMREARRK
                     VGPLGIEGDAWDVAQAVLFLASDEARFVNGVQLAVDGGVEGIGPLAGHALIGTP"
     gene            2238..3209
                     /locus_tag="VFF98_04115"
     CDS             2238..3209
                     /locus_tag="VFF98_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62845.1"
                     /translation="MADFALLALPGAYLGSVGALTDCFGLARGRVEQMFAGPDRLRMQ
                     TRLHRLSADGAPVVLADGMVLPVDGAIGSQGELAFIWLPAFVAGGRRQMEERIARNQP
                     LLAWLRARHAAGATIGASGAAALLLMAAGLTDKLAVPVPRVLQPLARALFPRQPFEDR
                     LQLVDRGTLLFSGGIGHDLALIVRVMERSLSPAIARWLVSVTGLDGEEGHLLADDQLV
                     ARAQVWIEQHFAGPLNLADLAANLSTSTATLNRRFHRVLGMPPKAYVQQLRLEAATRM
                     LEKSDRPIDRIAEMLGFSDSRLFRAMFRQHTGMTASQWRAAHRAQPA"
     gene            complement(3237..4892)
                     /locus_tag="VFF98_04120"
     CDS             complement(3237..4892)
                     /locus_tag="VFF98_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZU62846.1"
                     /translation="MAADFDEIADFVIVGSGGGSMAAALYLKSIGKQPLILEKTDRFG
                     GSTAMSGGILWVPNNHLLARDGIADSHAAGRTYMDATVGMDAGPGASVARKEAYLRAG
                     PELVRWLEAQGMVWLRTEGWADYYDDRPGGCTRSRSVGAPLLDARLMGPLFDKLRLGP
                     MVMPLPTDKAGVIGLATRTPAGMWEGVKLLFRMWLVKRRGKPILSFGGALQGRMLMLA
                     DSAGVDMRTDHGVVELIEAGGRITGVVAEHGGQRRRIGARDGVLINAGGFSRNAGMRR
                     KYGPQPSSVDWTNANPGDTGEMIEIAERHGAALDLMDQAWWVPGTLPPGNNPGPALMH
                     NTDISKPHCIIVNRQGRRILNESGSYMENGQRLYASGVPAFVILDSRHRQRYAWGYFP
                     PGKTPQAWFDSGFLKVADTLDELARKAGIDPAGLADEVAKFNTYCQTGKDLDFNRGGR
                     GYDNWFGDPTVKPNPNLGQIARGPFYAMELVPGDVGTSGGIVTDEHARALRGDGSVIE
                     GLYATGNSTASVMGRCYPAAGASIGASYIFAWIAAQHAAGRAG"
     gene            complement(4893..6206)
                     /locus_tag="VFF98_04125"
     CDS             complement(4893..6206)
                     /locus_tag="VFF98_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62847.1"
                     /translation="MNQQSKLAVTPDRVPAQAYISRDYVAREKERLWPRVWQMACREE
                     EIPQPGDFYTYDIADESITLVRRPDGRISAWHNVCPHRGRRLTEGCGWMGKVHCRYHG
                     WQWHLDGRPLEVVDRQDWGSLLPDAEIALQPVQVASWGGWVFINMDPAAPSLEVFLGE
                     AKTRLDPYEIDRMRYVWRKRIHLPCNWKTAQEAFMEGYHVQTTHRQLLPYMDDYTYSQ
                     AVGPHAMFGYAPTALFGLPSPRLGNQGGDIRQGLHAFNTQIWETLKATATEEMLAAGA
                     RLMELPETASPMEVYIAFAQFHREEAARAGRPFPDITGEQLMAGGTDWNIFPNLVFLQ
                     QPTNVLFYRARPDGDDPDRCIFEINVLERFAEGQEPRDVVVEVAEDWRQVDWGLILSQ
                     DFSNMEEVQRGMKSRAFAAARPSPLQEVEISNFHRVYHEYLGGEG"
     gene            complement(6232..7386)
                     /locus_tag="VFF98_04130"
     CDS             complement(6232..7386)
                     /locus_tag="VFF98_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU62848.1"
                     /translation="MQFDLTEEQAMFRDSVAAFAQRHLAAGALERAHSDDYPWDVARL
                     MAEQGLIGITVPAEKGGLGGSLMDAVIAIETIAAVCPRSADVVQAGNFGAIRTFAQFA
                     SEAQQAQYLAPLLRGEGLISVAMTEPGAGSAVTELTTTATPDGDGWRINGQKIFTTHG
                     THATVFLVYVRFGPGTGNIGSVLIERGQEGFTFGKPVRFLSGEEWNPLFFDNVHVPAD
                     KVLLGPGGFKKQMAGFNVERIGNTARSLALGRYAFNAAVEHAKTRKQFGQALCAFQGL
                     QWKFAEMKLKLDAAQLLLYRAASNADAGLPSPEETAIAKVACNRAGWDCANEALQVMG
                     GTGYSQDGLVEYCLRRTRGWMIAGGAIEVLLNRIAEGVFDMRFPQGPARS"
     gene            7605..>8448
                     /locus_tag="VFF98_04135"
     CDS             7605..>8448
                     /locus_tag="VFF98_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU62849.1"
                     /translation="MTAINDRISIRIDGDIGYLISHNPPVNALGIAVRQGLVAGLAAL
                     NADPAVRAIVLFCEGRTFFAGADITEFGKPRLSPTLQEVIAPLEASAAPVIAAIHGTA
                     LGGGFEVALGCPFRVGVATARVGLPEIALGLFAGGGGTQRLPRLIGPEAALELVLSGK
                     PVRAAEAQALGILDALGESDPAAVGTAFARRVIDERLPAVPLRLREEKIAATRADPAA
                     FEALAQRLTARSKGQLAPAANVASIRRAFTLPLDEALEIDAAANRELMAGSQSRALRH
                     LFFAE"
BASE COUNT         1270 a         3027 c         2784 g         1367 t
ORIGIN      
        1 ccgcctacac gccgatgtcc gccggcagca tcgatccgcg ctgggccgat tatgccgcgc
       61 ccgaacatgt ggcgccggtg gcaggctggc tgtgcagcga caggtgccgc acatcgggcg
      121 gcatctatca caccggcgcc ggccatgtgc gccgggtgca gctgctggaa gggccggtgc
      181 acaggctggc cgacggcgat atcgccagcg tgatgcaagg catcgccgcc ctgccggaat
      241 ggagcagttc cttcgcctcg ggcgcgcaga tcctgcccgc catcgccgct gccgccacca
      301 tcggagcatc cgcgcatgac gattgatttt accgggcagg tggcgatcgt caccggggcg
      361 ggcggcggca tgggccgggc ctatgcgctg gaactggcgc ggcggggcgc ggcggtgctg
      421 gtgaatgatt atggcggcag cgtgctgggc cagcaggatg gcagcccggc gcccgccgaa
      481 gcggtggccg ccgagatccg cgatctgggc ggccgggccc gggcctgccc gctggcggtg
      541 ggcagcgccg atcatgcccg cgccatcgtc gcctccgcgc tggaggcctt cggccggctg
      601 gacatcctcg tcaacaatgc cggcaccgcc ctggccggcc ccttcaccgc ccatgacgat
      661 gccgcggtgg aacgccatta tgccgtcaac cttgtcggcg gccatcacct gatgcgcgcc
      721 gcctggccgc atatggccgg ccagcgctat ggccgggtgc tcaatgtcag ctccaatggc
      781 gcgctggggg tgggcggcaa tgcgccctat gcggcggcca aggcgggcat gctgggcctc
      841 acgctggatg ccgcgataga ggggcggccg ctgggcattc tcgtcaatgc ggtgatgccc
      901 accagccatt cgcggatgat cgagcagatc cccgatccgg ccacggtggc atggtttcgc
      961 acccatctgc acacagggca ggtggccgcc gccatggcct ggttcctcag ccgcgcctgc
     1021 accgaaagcg ggcgcatcct gctggctggc ggcgggcgcc tctccagcct ggtgttcgcc
     1081 gagacgggcg agatcttcac ccccgcgctg gcggccgagg atgtcgccgc gcgcatcgcc
     1141 gaggtgaccg acgagcgcgt gctcagcccc gttcacagcc aggccgaagc cggcgcccgc
     1201 tatttccgcc acctgccctg gacaggggag agcgccgccg atttcggccc ggatgtgccg
     1261 cgcggcgcct gatgcgggcc ggcaccagcg gccgctcagg gcgtgccgat cagcgcatgg
     1321 ccggccagcg ggccaatccc ttccaccccg ccatccaccg ccaattgcac gccgttcacg
     1381 aagcgcgcct cgtcgctggc caggaacagc accgcctgcg ccacatccca ggcatccccc
     1441 tctattccca gcgggcccac cttgcggcgc gcctcgcgca tctcggcggg caccttggcc
     1501 atggcgtggg gggtcatcac atggccgggg gccaccgtgt tcacccggat gccctggcgg
     1561 ccatgcagca cgccaatctc gcgcgagagc tgcgccatcg ccgctttcga cgggccatag
     1621 gcaatcgccc ccatcgcccg catccccgcc agcgacgaga tgttgacaat ggccccgccg
     1681 ccttgcccgg ccatatgcgg cacggcatat ttgctcatca gcatggcgct ggtgaggttg
     1741 gccgccagcg tggcgttcca cagctccagc gtgatatcgg caaggctggt gggcacggca
     1801 atgccgacat tgttgaccag cacgtcgatc cgccccagcc ccgccacgca gccctccacc
     1861 agcgccgcgc aatcggccgg ccgggcaaca tcgccggcca tcgccagcgc ctggccgccg
     1921 ccggcgcgga tcatcgccgc ggtggcctct gcccgggcgc catcgaggtc ggcgcacagc
     1981 acccctgccc cctcgcgcgc cagcagcagc gcaatcgccc ggccaatgcc gatctcgccg
     2041 gcctcggccc ccgcgccggt gaccagcgcc accttgccgg acagacgatc gagataacgg
     2101 cttggctctg gcatcgcatt ctcccttgcg ccgcccatgc cggcgggcgc ctgttttcgg
     2161 cctgtataac aggcaatcac cgcccccatt gacaggtcgt ttttacgtga ttaccggatg
     2221 tttctgatca tactcccatg gccgatttcg ctcttctcgc gcttccgggc gcctatcttg
     2281 gcagcgttgg cgcgctgacg gattgctttg gcctggcccg cggccgggtg gagcagatgt
     2341 tcgccgggcc agaccggctg cgcatgcaaa cccgcctgca ccggctgtcg gccgatggtg
     2401 cgccggtagt gctggccgat ggcatggtgc tgccggtgga tggcgccatt ggcagtcagg
     2461 gcgagctggc cttcatctgg ctgcccgcct ttgtggccgg cgggcgccgg cagatggaag
     2521 agcgcatcgc ccgcaaccag ccgctgctgg cctggctgcg cgcgcgccac gcagcgggcg
     2581 ccaccattgg cgccagcggg gccgcagcgc tgctgctgat ggccgccggc ctgaccgaca
     2641 agctggccgt gccggtgccg cgcgtgctgc agccgctggc ccgcgcgctg ttcccccgcc
     2701 agcccttcga ggaccggctg cagctggtgg accgcggcac gctgctgttt tccggcggca
     2761 tcggccatga tctggcgctg atcgtgcggg tgatggaacg cagcctgtcg ccggcgattg
     2821 cccgctggct ggtctcggtc accggcctcg atggcgagga aggccatctc ctggccgacg
     2881 accagctggt ggcccgcgcg caggtgtgga tcgagcagca ttttgccggc ccgctcaacc
     2941 ttgccgatct ggctgccaac ctgtccacca gcaccgccac gctgaaccgg cgcttccacc
     3001 gcgtgctggg catgccgccc aaagcctatg tgcagcagct gcggctggag gcggccaccc
     3061 gcatgctgga aaaatccgac cggccgatcg accgcatcgc cgaaatgctg ggcttttccg
     3121 acagccgcct gttccgcgcc atgttccgcc agcacaccgg catgaccgcc agccagtggc
     3181 gggccgcgca tcgcgcccag cccgcctgaa ggcccttcaa cccgcctgaa ggcccttcag
     3241 cccgcccggc ccgcggcatg ctgggccgcg atccaggcga agatatagga cgcgccgatg
     3301 ctggcgcccg ccgccggata gcagcgcccc atcaccgatg cggtggaatt gcccgtggca
     3361 tacagcccct cgatcaccga tccatcgccg cgcagcgccc gggcatgctc atcggtgacg
     3421 atgccgcccg aggtgccgac atcgcccggc accagttcca tcgcatagaa cggcccccgg
     3481 gcgatctggc ccaggttggg gttgggcttc accgtgggat cgccgaacca gttgtcatag
     3541 ccgcgcccgc cgcggttgaa gtcaaggtcc ttgccggtct ggcaataggt gttgaacttc
     3601 gccacttcat cggccagccc ggcagggtct atgccggcct tgcgggccag ctcgtccagc
     3661 gtgtcggcca ccttgaggaa gccgctgtcg aaccaggcct gcggggtctt gccggggggg
     3721 aaatagcccc aggcatagcg ctggcggtgc cggctatcga ggatgacaaa ggccggcacg
     3781 ccgctggcat acagcctttg cccgttctcc atataggagc cgctctcgtt gaggatgcgc
     3841 cggccctggc ggttgacgat gatgcaatgc ggcttggaaa tatcggtgtt gtgcatcagc
     3901 gccgggccgg ggttgttgcc cggcggcaag gtgcccggca cccaccaggc ctgatccatc
     3961 aggtcgagcg cggcgccatg gcgttcggcg atctcgatca tctcgccggt atcgccgggg
     4021 ttggcgttgg tccagtcgac agagctgggc tgggggccat atttgcggcg catcccggca
     4081 ttgcgggaaa agccgccggc gttgatcagc acgccatcgc gcgcgccaat gcgccggcgc
     4141 tgcccgccgt gttcggccac cacgccggtg atccgcccgc ccgcctcgat cagctcgaca
     4201 acgccatggt cggtgcgcat gtccaccccg gcgctgtcgg ccagcatcag catgcgcccc
     4261 tgcagcgcgc cgccaaagct gaggatcggc ttgccccgcc gcttcaccag ccacatgcgg
     4321 aacaacagct tcacgccttc ccacatgccc gccggcgtgc gggtggcaag gccgatcacg
     4381 cccgccttgt cggtgggcag cggcatcacc atcgggccaa ggcgcagctt gtcgaacagc
     4441 gggcccatca gccgcgcatc aagcagcggc gcgcccaccg agcgcgagcg cgtgcagccg
     4501 cccggccggt cgtcgtagta atcggcccag ccttcggtgc gcagccacac catgccctgc
     4561 gcttccagcc agcgcaccag ctctggcccg gcgcgcagat aggcctcctt gcgcgccacc
     4621 gaggcgcccg ggccggcatc catgcccacc gttgcatcca tgtaggtgcg gccggccgca
     4681 tggctgtcgg caatgccatc gcgcgcgagg aggtggttgt tgggcaccca caggatgccg
     4741 cccgacatcg ccgtggaacc gccgaagcgg tccgtcttct caaggatcag cggctgcttg
     4801 ccgatggatt tgagatagag cgccgccgcc atcgaaccgc cgccagaccc cacgatgacg
     4861 aaatcggcga tctcgtcgaa atcggccgcc attcagccct ccccgccgag gtattcatgg
     4921 tagacccggt ggaaattgct gatctccact tcctgcagcg ggctgggccg ggccgcggcg
     4981 aaagcgcgcg atttcatgcc gcgctgcact tcctccatat tgctgaaatc ctgcgacagg
     5041 atcagccccc aatcgacctg gcgccaatcc tcggccacct ccaccaccac atcccttggc
     5101 tcctgccctt cggcgaagcg ttccagcacg ttgatctcga agatgcagcg atcggggtcg
     5161 tcgccatcgg gccgggcgcg atagaacagc acgttggtgg gctgctgcag gaagacaagg
     5221 ttggggaaga tgttccagtc ggtgccgccg gccatcagct gctcgccggt gatatcgggg
     5281 aacgggcggc cggccctggc cgcttcctcg cggtggaact gggcgaaggc gatataaacc
     5341 tccatcgggc tcgcggtttc gggcagctcc atcagccggg cgccggcggc cagcatttcc
     5401 tcggtggcgg tggccttcag cgtttcccag atctgggtgt tgaaggcatg cagcccctgg
     5461 cggatatcgc cgccctgatt gcccagccgg ggcgatggca ggccaaacag cgcggtgggc
     5521 gcatagccga acatggcatg ggggcccacc gcctgcgaat aggtgtaatc atccatatag
     5581 ggcagcagct ggcggtgggt ggtctgcacg tgatagcctt ccatgaaggc ttcctgggcg
     5641 gtcttccagt tgcagggcag gtggatgcgc ttgcgccaca catagcgcat ccggtcgatc
     5701 tcatagggat cgagccgcgt cttcgcctcg cccaggaaga cctccagcga aggcgctgcc
     5761 ggatccatgt tgatgaacac ccagccgccc cagctggcca cctgcaccgg ctgcagggcg
     5821 atctcggcat cgggcagcag gctgccccaa tcctgccggt cgaccacctc cagcgggcgg
     5881 ccatcgaggt gccactgcca gccatgatag cggcaatgca ccttgcccat ccagccgcag
     5941 ccctcggtca ggcggcggcc gcgatgcggg cagacattgt gccaggcgct gatcctgcca
     6001 tcggggcggc gcaccagggt gatcgattca tcggcgatgt cgtaggtgta gaaatcgccc
     6061 ggctggggga tttcctcctc gcggcaggcc atctgccaca cccgcggcca cagccgctcc
     6121 ttttcccggg ccacgtaatc gcgcgagata taggcctgcg ccggcacgcg gtcgggcgtc
     6181 acggcaagct tgctctgctg gttcatcctg tcctctcctt tcgccccggt gtcagcttct
     6241 ggcaggcccc tgggggaatc gcatgtcgaa tacaccctcc gcgatgcggt tcagcaagac
     6301 ctcgatcgcc ccgcccgcga tcatccagcc gcgcgtgcgg cgcaggcaat attccaccag
     6361 cccatcctgg ctgtagccgg tgccgcccat cacctgcagc gcctcattgg cgcaatccca
     6421 gccggcgcgg ttgcaggcca ccttggcaat cgccgtctcc tcgggcgagg gcagccccgc
     6481 atcggcattg ctggccgccc gataaagcag caattgcgcc gcatcgagct tcagcttcat
     6541 ttcggcaaac ttccactgca acccctggaa ggcacacagc gcctggccga actgcttgcg
     6601 ggttttggca tgttccaccg ccgcgttgaa ggcatagcgg cccagcgcca ggctgcgcgc
     6661 ggtgttgccg atgcgctcca cattgaagcc ggccatctgc ttcttgaagc cgcccggccc
     6721 cagcagcacc ttgtcggccg gcacatgaac attgtcgaag aacagcgggt tccattcctc
     6781 gcccgaaagg aagcgcacgg gcttgccgaa agtgaagcct tcctgcccgc gctcgatcag
     6841 caccgagccg atattgccgg tgcccggccc aaagcgcaca taaacgagga acaccgtggc
     6901 atgggtgcca tgggtagtga agatcttctg cccgttgatc cgccagccat cgccatcggg
     6961 cgtggcggtg gtggtcagct cggttacggc agagccggcg cccggttctg tcatcgccac
     7021 cgagatcagc ccctcgccgc gcagcagcgg cgccagatac tgcgcctgct gcgcctcgct
     7081 ggcgaattgg gcgaaggtgc ggatggcgcc gaaattgccc gcctgcacca catcggccga
     7141 ccgcgggcag accgcggcga tcgtctcgat ggcgatcacc gcatccatca gcgagccgcc
     7201 cagcccgcct ttttccgccg gcacggtgat gccgatcagc ccctgttcgg ccatcagccg
     7261 ggccacatcc cagggataat cgtcgctatg ggcccgctcc agcgcgccgg cggccagatg
     7321 gcgctgggcg aaggcggcca cggaatcgcg gaacatcgcc tgttcttcgg tcagatcgaa
     7381 ctgcattttc tcaccccatc cgcgcaggcc ttcccgcgct tctcaaccgc agtcgcctaa
     7441 tgcgcggcag gggctgtgcg aagggcgcgg gccaaccctt cgcattcgtg atgcatcacc
     7501 cccgtttccc gccggtttct tgacagtgcg cgcccctcgc agcttcaaac gcggggcgag
     7561 ccgggccgca agggcgggca tcaacagggc gcaagtattc agccatgaca gcgatcaacg
     7621 acaggatttc catccgtatc gacggcgata tcggatatct catcagccac aatccgccgg
     7681 tcaacgcgct gggcattgcc gtgcgccagg ggctggtggc ggggctggcc gcgctgaacg
     7741 cggatccggc ggtgcgggcc atcgtgctct tctgcgaggg gcgcaccttc tttgccgggg
     7801 cagacatcac cgaattcggc aagccgcgcc tctcgcccac gctgcaggaa gtgatcgccc
     7861 cgctggaagc ctcggccgcg ccggtgatcg ccgcgatcca cggcacggcg ctgggcggcg
     7921 gcttcgaggt ggcgctgggc tgcccgttcc gcgtgggggt ggccaccgcc agggtgggcc
     7981 tgccggaaat tgccctgggc ctgtttgccg gcggcggcgg cacccagcgc ctgccccggc
     8041 tgatcggccc ggaagcggcg ctggaactgg tgctgtcggg caagccggtg cgggcggcag
     8101 aagcgcaggc gctgggcatt ctcgatgcgc tgggcgaaag cgatccggcg gcggtgggca
     8161 ctgcctttgc ccgccgggtg atcgacgagc gcctgccggc cgtgccgctg cgcctgcgcg
     8221 aggagaagat cgccgccacc cgcgccgatc cggctgcctt cgaggccctg gcccagcggc
     8281 tgaccgcgcg cagcaagggc cagctggcgc cggcggccaa tgtcgcctcg atccgccgcg
     8341 cgttcaccct gccgctcgac gaggcgctgg agatcgatgc cgccgccaac cgcgagctga
     8401 tggccggcag ccagtcgcgc gcgctgcgcc acctgttctt tgccgagc
//
LOCUS       DASRYE010000074        11242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_128105,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000074 DASRYE010000000
VERSION     DASRYE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128105"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFF98_04140"
     CDS             complement(<1..301)
                     /locus_tag="VFF98_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62850.1"
                     /translation="MEAQITLLRADLTFPESPRWHEGHVWCSDDHRVLRVSPAGEVQV
                     MARLDSHVLGIQCHDGALFAAIAHQRRIVRIDAQRQVTDHADLSPLFDRPMNEFVF"
     gene            complement(313..1074)
                     /locus_tag="VFF98_04145"
     CDS             complement(313..1074)
                     /locus_tag="VFF98_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62851.1"
                     /translation="MTSETIEQRLDAMTHEIDRLKAITAIQNTMSHYETVHCSGEIHR
                     TTECFALWRPDCTAEVAGWGCFVGTDTVKEFWGRQNRMDPRGAIFFHTLASPAIEVAG
                     DGKTAKATWASPGFEGARKDGDPLAFWCWGKYGADFIRNPATGEWKIWHLKWFRTIRN
                     HFNTPWPEESHQVMVGTINLGDHFADLNRVPSAFHRPYQSEEVAHPFPVTPQPYADYD
                     GNFHWMFGGAEMERQYGLDLPDYSRHYNAAFPDKI"
     gene            complement(1096..3480)
                     /locus_tag="VFF98_04150"
     CDS             complement(1096..3480)
                     /locus_tag="VFF98_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62852.1"
                     /translation="MPTCRRHLLTSVMVWPMLAATAHAQSSAAATPPGADAEIIVTAQ
                     YKGQSVQQVPLAITALSGATLEQRGLTNVSAVSAAVPNVLIAPTTNGPAVNLYIRGVG
                     QDDTTPAMEPGVGVYVDDVYYPTLVGSQLELLDLSRLEVLKGPQGTLAGRNSMGGALK
                     LYTKDPSGRRDAYVSVEYGSYNSVNLRAGTNFTLLPDALFARITGFYRSSEGYVTDYD
                     YNCTHKGSLGSFTATGANCVIGHDGGVNIGGIRGALLYDQGNGLKVRLSGEYIRQRNE
                     PNASTLLATAGNDGVGDNAPSQNGVPYSYSGTSAWAGSPFIAYSPYAGIAAGDSFTHS
                     PYVNYANYCNSSGRSGTNSYAGYCLSRKNNIDSYTASLAIDYRVAEKLTLKSITALSG
                     YKADYTDDGDKSPLFTDLTDTQFDNHYLSQEVRLSGTLRRLDFTVGGIYTDQATVQGG
                     TVDFSTFAMQQNDRIPLTTGALFAHADWRVAGGLHLIGGLRWTHEEKNYIFRRATING
                     QPNSTVPALIGFDNYTPAAYSGSHTDYRLALQYAFSHDIMAYVQTATGFKGGGVNPRP
                     FSVAQALPFGQEKLTAYEAGVKSMLFGRRLKLNAAIFFNDYTGKQLGITTCTIGLGPC
                     NVILNAGNVRESGQEVEFDLHPVPALSLHGSASWLQSHYTYLTSDAIGSGLTYGMAIP
                     YQIRSKFNLGADYRLDLGRAGSLTPSVDYAHQTGFYTTTVNIPVVGYNPAYGVWSARL
                     SWKNREGDLEVYVRAENLGDAVYYYNNYPATINMAVTGAIAPPREVFAGIKKTF"
     gene            complement(3573..4352)
                     /locus_tag="VFF98_04155"
     CDS             complement(3573..4352)
                     /locus_tag="VFF98_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62853.1"
                     /translation="MSETEWRRLERIVAAQQHELERAQAVTAIQNCMGRYETVHITPA
                     EIGRSGECFALWRADCSVEVSDWGCFFGGEAIDGFWRSMTAPDLRGGIFWHALATPVI
                     QVAGDGRTAKVTWMSPGFETMPPLQAGEEAKSFWCWGKYACDFIRHPETGAWKIWHMK
                     WFRTIRSDFAKSWYEDSRNTLAGMPGPVPYDHANRQPSVFHRPYQFDEVPHPFPIDPE
                     PYATYDGNFRWIYGGAEYEARYGVRHPDYARLYHVNYPNAV"
     gene            4580..5785
                     /locus_tag="VFF98_04160"
     CDS             4580..5785
                     /locus_tag="VFF98_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62854.1"
                     /translation="MTDRTPANGAIPRRPGLLQAAILIAAVIAGTMPMVIGPVLPAIQ
                     RHFSPAPGVALLVPMLVTTPSLITALLAGIMGLLSDKWGRRRLLIAAIILYGLAGTAP
                     LYLDNLPLIVATRALVGVAEAGAMTVSTIMIGDYFSGQRRDRIIAAQTTLASVSGIAI
                     SLMGGWLGEWGWRGPFLVYGAMVLLLPPMLLWLWEPARARRQQAAPAHLQGLATPGFI
                     GALAVSFLIGLGLMLIPVNLAYMLGEAGIGDPAAAGRVYAMACLGTVSGTLLFAHGLA
                     GRTAVALQFALCAALCAIGTAAIGQAHTLWALTGWSVLTSLGIGISLPAGTSWVLGML
                     SPAHRGRGVGLYMAAQFIGAFISPLIVVPLALWLHSYAKAVQTSAWVFAPLAAYALLS
                     QPALRWRAR"
     gene            complement(5755..6687)
                     /locus_tag="VFF98_04165"
     CDS             complement(5755..6687)
                     /locus_tag="VFF98_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62855.1"
                     /translation="MQSVQCNDANSWLGFLGEDRPIKAAMRAYGRFFAEGQIISSGSL
                     RLVRTHSTPHEISLTPPRLSRNAHMLSMVIQCAGRTEMRVGNHGWALSPGDWCVNGIN
                     ENCHLSHCGETEHLVVTIPKSALQLDEARIARLGRRHFAGNQGLSRLMCDMAMGQFRE
                     APYMGTQAANASSACLLGLMRSVLSEEATASARGHARGELVEAIKDFIGHNLTNAMLS
                     IDVIARELGYSKRYLHMAFSQGMPGTTISDHILNERLLACEQDLLYFSGAARPVSEIG
                     QAWGFVDPAHFSRVFRNRYGVPPSVYRARHRKAG"
     gene            complement(6845..6921)
                     /locus_tag="VFF98_04170"
     tRNA            complement(6845..6921)
                     /locus_tag="VFF98_04170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6885..6887),aa:Arg,seq:tct)
     gene            7032..7775
                     /locus_tag="VFF98_04175"
     CDS             7032..7775
                     /locus_tag="VFF98_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZU62856.1"
                     /translation="MSHEPHLRHVDASALAHKPMRYFDFMMAAFVSILLLSNVIGAGK
                     VARVNLPIIGDWPFGAGILFFPLGYLLGDILTEVYGYARARRCVWVGFFALLFMAFMS
                     WVVVSLPPATGWTGQAAYDQVFGQVPRIVLASITAFWGGEFANSFVLARMKVWTRGEK
                     LWTRTIGSTIVGQAVDSIVFYPLAFLGASGWTPALVGKVLITNWALKVGWEVLLTPLT
                     YAVVGFLKRAEGVDVFDEKTNFSPFRTEV"
     gene            complement(7785..8762)
                     /locus_tag="VFF98_04180"
     CDS             complement(7785..8762)
                     /locus_tag="VFF98_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU62857.1"
                     /translation="MDLVNGAGQASRLSVAVLLPAYNEAAAIAQVIADFRAALPDAAI
                     YVYDNNSRDETCAIARAHGAIVRREPRQGKGHVVRRMFADIEADVYIMCDADDTYEAA
                     AAPDMLRRLLDEGLDMVVGARAAVSETAYRPAHEFGNWLLTGLVRTIFGDVFRDMLSG
                     YRVFSRRFVKSFPVMAGGFEIETELTIHALELEMPVAEQRTRFQDRPAGSVSKLNTIR
                     DGVRILWTILALLKQERPMPLFGLIGAALMLLSLGAAWPVVMEYLATGLVPRLPTAVL
                     ATGLMLLGWFALFSGLILDTVTRGRQEAKRLRYLAFPGTHAWQGAGEEG"
     gene            8896..9999
                     /gene="recF"
                     /locus_tag="VFF98_04185"
     CDS             8896..9999
                     /gene="recF"
                     /locus_tag="VFF98_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZU62858.1"
                     /translation="MGLARITLTHFRNHAATCVEGTAGFNLLVGENGAGKTNVLEAIS
                     LLAPGRGLRRAPLADMANRAGEGGFAISAELAGGGLRLGTGTAPDRPGRRLVRINGAD
                     ASALALSEWLALAWLTPAMDRLFADSPGARRRFMDRMALAIAPAHATHAARTEAALRE
                     RNRLLAAEHAPDPAWLDAVEAQLAEAGAALTAGRARLVEALAALIERLPPSPFARPVL
                     ALAPGGPDEAGALAEALRAARPRDRAAGRSLTGPQRDDLLVTMAAKNQPAADCSTGEQ
                     KAMLIAITLAHAELAAMLDRAGDAPPRARLLLLDEVAAHLDPIRRGALFERLAHSGAQ
                     VWLTGTEPAPFADLQGRAACWRVTEGRVLPITL"
     gene            complement(9989..>11242)
                     /locus_tag="VFF98_04190"
     CDS             complement(9989..>11242)
                     /locus_tag="VFF98_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-3-deoxy-D-manno-oct-2-ulosonic acid
                     transferase"
                     /protein_id="HZU62859.1"
                     /translation="PATCELALVAALLGRPLEVAGAGPYAGLAQGPQGLLAVLAREVI
                     LPWRYACPFTGAEMGALAAARLLGQWRRLIDGNRPVGAIHGVARWKRGTLDALLWAGR
                     GRPPYGGQGKGRVLVWQARTPARVLARLAGPVGEIEDGPIRSAGLGAHCVPPLSIVVD
                     ELGPYFDPARESGLERLLARAAFPPDLLARAAALRGAIVAGGVGKYGAGGGALAAWPA
                     DGRRRVLVVGQVADDRAMALGGAGCTNLALLQAARRLEPQAHIIWRPHPDVVAGLRPG
                     ALPPDQVRALADAEAAEAPIAGLLAGADAVHVISSLAGFEALLRGCAVTVHGLPFYAG
                     WGLTRDLAITPARRRRRLALDELVAAALILYPRYLDPLTRLPCPPEVLVARLVAGVGQ
                     RGLGWPLVALRRWQGRLRRWLAARA"
BASE COUNT         1814 a         3879 c         3680 g         1869 t
ORIGIN      
        1 cgaagacgaa ctcgttcatc ggccggtcga acagggggct gaggtcggca tggtcggtga
       61 cctgccgctg cgcgtcgatg cggacgatcc ggcgctgatg ggcgatggcg gcgaacagcg
      121 cgccatcatg gcactgtatc cccagcacat ggctgtccag cctcgccatg acctgcacct
      181 cgcccgcggg cgagacgcgc agaacgcggt gatcatcgga acaccagaca tggccctcat
      241 gccagcgcgg gctttcggga aaggtcaggt ccgcgcgcag cagcgtgatc tgtgcctcca
      301 tggccggctc catcagatct tgtcggggaa ggcggcgttg tagtggcggc tgtaatccgg
      361 cagatcgagg ccatattgcc gctccatctc cgcgccgccg aacatccagt ggaagttgcc
      421 gtcgtaatcc gcatagggct gcggggtgac cgggaaggga tgggcaacct cctcgctctg
      481 ataggggcgg tggaaggcgg agggcacgcg gttcagatcc gcaaagtggt cgccaaggtt
      541 gatcgtgccg accatcacct gatggctttc ttccggccag ggcgtgttga aatggttgcg
      601 gatggtgcgg aaccatttga gatgccagat tttccattcg cccgttgccg ggttgcggat
      661 gaagtccgcg ccatatttgc cccagcacca gaaggccagc ggatcgccat ccttgcgcgc
      721 gccttcaaag ccgggggagg cccaggtggc cttggcggtc ttgccatcgc cggccacctc
      781 gatggcgggc gaggccagcg tgtggaagaa gatcgcgccg cgcgggtcca tcctgttctg
      841 gcggccccag aattccttca ccgtatcggt gccgacgaag cagccccagc cggccacctc
      901 ggcggtgcaa tcaggccgcc acagggcgaa acattcggtg gtgcggtgga tctcgcccga
      961 gcaatgcacg gtttcataat gcgacatggt gttctggatg gcggtgatcg ccttcagccg
     1021 gtcgatctcg tgcgtcatgg cgtcgaggcg ctgctcgatg gtttcactgg tcatggaaat
     1081 gcctttggtt gagggtcaga aggtcttctt gattccggcg aagacttcgc gcgggggcgc
     1141 aatggcgccg gtcacggcca tgttgatggt cgcgggatag ttgttgtagt aatacaccgc
     1201 atcacccagg ttctcggcgc ggacatagac ttccagatcg ccctcgcggt tcttccagct
     1261 caaccgcgcg ctccacacgc cataggccgg gttgtagccg acgaccggga tgttgaccgt
     1321 ggtggtgtag aagccggtct ggtgggcata gtccacgctg ggcgtcaacg agcccgcgcg
     1381 gcccagatca agccggtaat cggcgcccag gttgaacttg ctgcggatct gataggggat
     1441 ggccatgcca taggtcaggc cgctgccgat cgcgtcgctg gtgagatagg tgtagtgcga
     1501 ttgcagccag ctggccgagc catgcagcga gagggccggc accggatgca ggtcaaactc
     1561 cacctcctgc ccgctttcgc gcacattgcc cgcgttgagg atgacattgc aggggcccaa
     1621 accgatggtg caggtggtga tgcccagctg cttgccggtg taatcgttga agaagatcgc
     1681 cgcgttgagt ttcagcctgc ggccgaacag catggacttc acccccgctt cataggcggt
     1741 cagcttttcc tggccgaagg gcagcgcctg ggccacgctg aaggggcgcg gattgacgcc
     1801 gccccccttg aagccggtcg ccgtctgcac ataggccatg atgtcgtggc tgaatgcata
     1861 ttgcagcgcc aggcggtagt cggtgtgcga gccgctgtag gcagcgggcg tgtaattgtc
     1921 gaagccgatg agcgccggga cggtgctgtt gggctggccg ttgatggtgg cgcggcggaa
     1981 gatgtagttc ttctcctcat gcgtccagcg caagcccccg atcagatgca gcccgcccgc
     2041 cacgcgccag tcggcatggg cgaagagcgc gccggtggtc agcggaatgc ggtcgttctg
     2101 ctgcatggcg aaggtggaga aatcgaccgt gccgccctgc accgtcgcct gatcggtgta
     2161 gatgccgccc accgtgaaat cgagccggcg caaggtgcca ctcagccgca cttcctgcga
     2221 gagatagtgg ttgtcgaact gggtgtcggt cagatcggtg aacaggggcg atttatcgcc
     2281 atcatcagtg taatcggcct tgtagccaga cagggcggtg atcgatttca gcgtcagctt
     2341 ctccgccacc cggtaatcga tggccagcga tgccgtgtag ctgtcgatgt tgttcttgcg
     2401 cgacaggcaa tagcccgcat aggaattggt tccgctgcgg ccactgctgt tgcaatagtt
     2461 tgcgtagttg acataggggc tgtgcgtaaa actgtcgccc gcggcaatgc cggcataggg
     2521 cgaataggca atgaagggcg aaccggccca ggcgctggtg cccgaatagc tgtagggcac
     2581 gccattctgg ctcggcgcat tatcgcccac gccatcattg ccggcggttg ccagcagggt
     2641 ggaggcattc ggctcgttgc gctggcggat atattcgcca gacagcctga ctttcagccc
     2701 attgccctga tcgtagagca atgcgccccg aatgccaccg atgttgacgc cgccatcatg
     2761 gccgatcaca caattggcgc cggtcgcggt aaagctgccc aggctgccct tgtgcgtgca
     2821 attatagtca taatcggtaa cgtaaccctc gctgctgcga tagaaaccgg tgatgcgcgc
     2881 gaagagcgca tcgggcagca gcgtgaaatt ggttcctgcg cgcaggttga cgctgttgta
     2941 gctgccatat tccaccgaaa cataagcatc gcgccggccc gagggatcct tggtgtagag
     3001 cttgagcgcc ccgcccatcg aattgcgccc ggccagcgtg ccctgcggcc ccttgagcac
     3061 ttccagacgg ctgagatcga gcagttcgag ctgcgatccc accagcgtgg ggtaatagac
     3121 atcatcgaca taaacgccca cgcccggctc catcgccggc gtcgtgtcat cctgccccac
     3181 gccgcggatg tagaggttga ccgccgggcc attggtggtc ggcgcgatca gcacattggg
     3241 cacggcggcc gaaacggcgc tgacattggt gagcccgcgc tgctccagcg tggcgccgga
     3301 cagggcggtg atggccagcg gcacctgctg caccgattgc cccttgtact gcgcggtgac
     3361 gatgatttcg gcatcggccc cgggcggcgt ggcggccgct gaggattgcg catgggctgt
     3421 ggcggccagc atcggccaga ccatcacgct ggtgagcaga tggcgacgac aagtcggcat
     3481 gatgagtatc ctttccctga gtgagcgccc gctttgcgcg ggtcgccaag gccagccggc
     3541 cgcatggggc cggcgcgtcg cacgccgccg cttcagacgg cgttcgggta gttcacatga
     3601 tagagccggg cataatcggg gtgccgcacg ccatagcgcg cctcatactc cgccccgcca
     3661 tagatccagc ggaagttgcc gtcgtaggtg gcatagggtt cgggatcgat cgggaaggga
     3721 tgcggcacct cgtcaaactg gtagggccgg tggaagaccg agggctgccg gttggcatga
     3781 tcatagggca caggccccgg catgccggcc agcgtgttgc ggctgtcctc gtaccagctt
     3841 ttggcaaaat cgctgcggat ggtgcgaaac cacttcatgt gccagatctt ccacgcaccg
     3901 gtttccgggt ggcggatgaa atcgcaggca tatttgcccc agcaccagaa gctcttcgcc
     3961 tcctctccgg cctgcagcgg cggcatggtt tcaaagccgg gcgacatcca cgtcaccttg
     4021 gccgtgcgcc catcgcctgc cacctggatc actggcgttg ccagcgcgtg ccagaagatg
     4081 ccgccgcgca gatccggcgc ggtcatgctg cgccagaagc cgtcgatcgc ctcgccgccg
     4141 aagaagcagc cccagtcgga cacctccacg ctgcaatccg cgcgccacag ggcaaagcat
     4201 tcgccgctcc ggccgatttc ggcgggggtg atgtgcacgg tttcatagcg ccccatgcag
     4261 ttctggatgg cggtgacagc ctgcgcccgc tccagttcat gctgctgggc ggccacgatc
     4321 cgctccagcc ttctccattc ggtttcgctc atgtcggccc ctcctgacgc ctacttatcc
     4381 cgcgcgcacc ggcggctcca tggctgggaa cgaagtcctc ttggagcata gggaactgcg
     4441 gcggagcggc cccggcgcgc catttccccg tggtccaacc ggcatttccc ccggcgacat
     4501 ggccgcgcgc gccgggggca tgctaggctg gccgccagca agccgggaga gccggcacaa
     4561 gacatgaggg agagatggat tgaccgatcg aacgcccgcc aatggcgcaa tcccccggcg
     4621 gcccgggctg cttcaggcgg cgatcctgat cgcggcggtc atcgcgggca ccatgcccat
     4681 ggtgatcggc ccggttctgc ccgcgatcca gcgccatttc agccccgccc cgggggtggc
     4741 gctgctggtt cccatgctgg tgacaacgcc ttcgctgatc acggccctgc tggccgggat
     4801 catgggcctg ctctcggaca aatggggccg gcgcaggctg ctgatcgcag cgatcatcct
     4861 ctatggcctg gccggcaccg ccccgctcta cctcgacaat ctgcctctca tcgtcgccac
     4921 gcgcgcattg gtgggcgtgg ccgaggccgg cgccatgacg gtcagcacga tcatgatcgg
     4981 cgactatttc tccggccagc ggcgcgaccg catcatcgcg gcgcaaacca cgctggcctc
     5041 cgtctcgggc attgcgatca gcctgatggg cggatggctg ggggaatggg gctggcgcgg
     5101 gccgttcctg gtgtatggcg ccatggtgct gctgctgccg cccatgctgc tgtggctgtg
     5161 ggaaccggcc agagcgcgcc gccagcaagc agcgcccgcc catctgcagg gccttgccac
     5221 accagggttc atcggcgctc tggcggtttc ctttctcatc gggctggggc tgatgctgat
     5281 cccggtcaac ctggcctata tgcttggcga ggcggggatc ggcgatccgg ccgccgcggg
     5341 gcgggtctat gccatggcct gccttggcac ggtctcgggc acgctgctgt ttgcccatgg
     5401 gctggcgggg cgaacggcgg tggcgctcca gttcgccctg tgcgccgcgt tgtgcgcgat
     5461 cggcacggca gcgatcggcc aggcgcatac gctatgggcc ctcactggct ggtcggtgct
     5521 gaccagcctt ggcatcggca tctcgctgcc cgccggaacg agttgggtgc tgggcatgct
     5581 atcgcccgcg catcgcggcc ggggcgtggg cctctacatg gcggcgcagt tcatcggcgc
     5641 cttcatcagc ccgctgatcg tggtgccgct ggcgctttgg ctgcactcct atgcaaaggc
     5701 ggtgcagacc agcgcctggg tcttcgcgcc gctggcggcc tatgccctgc tgtctcagcc
     5761 cgccttgcga tggcgcgcgc gatagaccga cggcggcacc ccataccggt tgcgaaagac
     5821 ccggctgaaa tgggccgggt cgacaaagcc ccaggcctgc ccgatctcgc tgaccgggcg
     5881 agccgcgccg gaaaaataga gcaggtcctg ctcgcaggcc aacaggcgct cgttcagaat
     5941 gtggtcggag atggtcgttc ccggcatgcc ctggctgaag gccatgtgga gatagcgttt
     6001 gctatagccc agttcacgcg cgatgacatc gatcgagagc atcgcattgg tcagattgtg
     6061 cccgatgaaa tccttgatcg cctccaccag ttcgccgcgt gcatggcccc gggccgaggc
     6121 tgtggcctcc tcgctcagca cgctgcgcat caggcccagc aggcaggcgc tcgaggcgtt
     6181 ggccgcttgc gtgcccatgt aaggcgcctc gcggaactgg cccatggcca tatcgcacat
     6241 cagccgggac aacccctgat tgcccgcgaa atggcgccgg ccaaggcgcg cgatgcgggc
     6301 ctcatcaagc tgcagcgccg atttggggat ggtcaccacc agatgctcgg tttcgccgca
     6361 gtggctgagg tggcagttct cgttgatgcc gttgacgcac cagtcgccgg gcgacagggc
     6421 ccatccatgg ttgcccacgc gcatttcggt gcggcccgcg cattgaatga ccatcgagag
     6481 catatgggca ttgcgcgaca gccggggcgg tgtgagcgag atttcatgcg gcgtggaatg
     6541 cgtgcgcaca agccgcaacg agccgctgct gatgatctgc ccttccgcga aaaagcggcc
     6601 ataggcccgc atcgccgcct tgatcgggcg gtcctcaccg aggaaaccca gccagctgtt
     6661 cgcatcattg cactgcacgc tctgcatgcg atgtcctcca cccgatattt ttatctcata
     6721 agccccactc tacccgacag ctttacagcc gcaagccgaa tgacaggcgc ccctgatggg
     6781 gaccggatgg cgatgcaccg ccgcctctgg cgtaaaggcg gcggcgcgcg gttctggagc
     6841 gaaatggcgc gcccgacagg gttcgaacct gtgaccccaa gcttagaagg ctcgtgctct
     6901 atccagctga gctacgggcg cgcaaaccct tgccccaata gccctctctt gcacggggcg
     6961 taaagccggt tactgtgcgc cgcgtcattt cttgatgaca ccgcccttcc cagcccttcg
     7021 gatcccccct catgagccac gagccccacc tgcgccacgt tgatgccagc gcccttgccc
     7081 acaagccgat gcgctatttc gatttcatga tggcggcctt tgtctccatc ctgctgctgt
     7141 ccaatgtgat cggcgcgggc aaggtggcca gggtcaacct gcccatcatt ggcgactggc
     7201 cgtttggcgc gggcatcctg ttcttcccgc tgggctatct gctgggcgat atcctcaccg
     7261 aggtctatgg ctatgcccgc gcgcggcgct gcgtgtgggt gggctttttc gcgctgctgt
     7321 tcatggcctt catgagctgg gtggtggtct cgctgccgcc ggccacaggg tggacggggc
     7381 aggccgccta tgatcaggtg tttggccagg tgccgcgcat cgtgctggcc agcatcaccg
     7441 ccttctgggg cggcgaattc gccaattcct tcgtgctggc gcgcatgaag gtgtggaccc
     7501 gcggcgaaaa gctgtggacc cgcacgatcg gctccaccat cgtcggccag gcggtggaca
     7561 gcatcgtctt ctaccccctc gccttcctcg gcgcatcggg ctggacaccg gcgctggtgg
     7621 gcaaggtgct gatcacgaac tgggcgctga aagtgggctg ggaagtgctg ctcaccccgc
     7681 tcacctatgc ggtggtgggc ttcctcaagc gggccgaggg cgtggatgtg ttcgacgaga
     7741 aaaccaattt ctcacccttc cgcaccgagg tctgaggggg ccgttcagcc ttcctcgccg
     7801 gcgccctgcc aggcatgggt gccgggaaag gccagatagc gcagccgctt ggcctcctgc
     7861 cgcccgcgcg tgacggtgtc gagaatcaac cccgagaaca gcgcgaacca gcccagcagc
     7921 atcagccctg tggccagcac ggcggtgggc aggcgcggca ccagcccggt ggccagatat
     7981 tccatcacca ccggccaggc cgcccccagc gacagcagca tcaacgccgc cccgatcagg
     8041 ccaaacagcg gcatcggccg ctcctgcttc aacagcgcca gaatggtcca caagatgcgc
     8101 acgccatcgc ggatggtgtt gagcttgctg accgagccgg ccggccggtc ctggaagcgg
     8161 gtgcgctgtt cggccaccgg catttccagc tccagcgcgt ggatggtcag ctccgtctcg
     8221 atctcgaagc cgccggccat caccgggaag ctcttcacga agcggcgcga aaacacccgg
     8281 tagccgctca gcatatcgcg gaagacatcg ccgaaaatcg tccgcaccag ccctgtcagc
     8341 agccagttgc caaattcatg ggccgggcga taggccgtct cgctcaccgc ggcgcgcgcg
     8401 cccaccacca tgtccagccc ctcgtccagc aggcggcgca gcatatcggg cgcggcggcg
     8461 gcctcatagg tgtcgtcggc atcgcacatg atatacacat ccgcctcgat atcggcgaac
     8521 atgcggcgca ccacatggcc cttgccctgg cgcggctcgc gccggacgat ggcgccatgg
     8581 gcgcgggcga tggcgcaggt ctcgtcgcgg ctgttgttgt cgtagacata gatcgccgca
     8641 tcgggcagcg ccgcgcggaa atcggcaatc acctgcgcga tggcggccgc ctcgttatag
     8701 gccggcagca acaccgccac agacagccgg ctcgcctgcc cagccccatt caccagatcc
     8761 atcgccaaac ccatcatcca caagctgaaa tgcgcccgcg ggctgatagc acacgccagc
     8821 cctttgtgaa gtggcgagcg cgcccgccat ccggccctat ggcgccccgc ccacacaatc
     8881 gctagagcag cgcgcatggg gctggcgcgc atcactctca cgcattttcg caaccatgcc
     8941 gccacctgcg tggaaggcac cgccggcttc aacctgctgg tgggcgaaaa tggcgcgggc
     9001 aaaaccaatg tgctggaagc gatcagcctg ctggcgccgg gccggggcct gcgccgcgcg
     9061 ccgctggccg atatggccaa ccgcgccggc gagggcggct ttgccatcag cgccgaactg
     9121 gccgggggcg gcctccggct gggcaccggc accgcgcccg atcggcccgg ccgccggctg
     9181 gtgcgcatca acggcgccga tgccagcgcg ctggcgcttt ccgaatggct ggcgctggcc
     9241 tggctgaccc cggcgatgga ccgcctgttt gccgacagcc cgggcgcacg ccgccgcttc
     9301 atggatcgca tggcgctggc gatcgcgccg gcccatgcca cccatgccgc ccgcaccgag
     9361 gcggccctgc gcgagcgcaa ccgcctgctg gcggccgagc acgcccccga tccggcctgg
     9421 ctggatgcgg tggaagccca gctggccgag gcgggcgccg cgctcaccgc cggccgggcg
     9481 cggctggtcg aggcgctggc cgcgctgatc gagcggctgc ccccctcgcc ctttgcccgc
     9541 ccggtgctgg cgctggcccc cggcgggcca gacgaggccg gcgccctggc cgaggccctg
     9601 cgcgccgccc gcccgcggga ccgcgccgcc ggccgcagcc tgaccggccc ccagcgcgac
     9661 gatctgctgg taacgatggc cgccaagaac cagcccgccg ccgattgctc caccggcgag
     9721 cagaaggcga tgctgatcgc catcaccctg gcccatgccg aactggccgc catgctcgac
     9781 cgcgccggcg atgcgccgcc ccgcgcgcgg ctgctgctgc tggatgaagt ggccgcccat
     9841 ctcgatccca tccgccgcgg tgccctgttc gagcggctgg cccacagcgg cgcgcaagtg
     9901 tggctgaccg gcaccgaacc tgcccccttt gccgatcttc agggccgcgc ggcctgctgg
     9961 cgggtgacag aagggcgcgt gctgccgatc acgctctagc ggccagccag cggcgcagcc
    10021 gcccttgcca gcgccgcagc gccaccagcg gccagcccag gccccgctgc cccacgcccg
    10081 ccaccagccg cgccaccagc acttcgggcg ggcaaggcag ccgcgtcagc ggatcgagat
    10141 agcgcggata gaggatcagc gcggccgcca ccagctcgtc cagcgccaac cggcggcgac
    10201 ggcgggcggg cgtgatcgcc agatcgcgcg tcaaccccca gccggcatag aaaggcaggc
    10261 catgcaccgt caccgcgcag ccccgcagca aggcctcaaa acccgccagc gagctgatca
    10321 catgcaccgc atcggccccc gccagcaggc cggcgatcgg tgcttcggcc gcctcggcat
    10381 cggccagcgc ccgcacctga tcgggcggca gggcgcccgg gcgcaggccc gccaccacat
    10441 cgggatgcgg ccgccagatg atatgcgcct gcggctccag ccggcgcgcg gcttgcagca
    10501 gggcgaggtt ggtgcagccc gcgccgccca gtgccatcgc ccggtcatcc gccacctggc
    10561 ccaccaccag cacccggcgt cggccatcag ccggccaggc cgccaaagcg cccccgcccg
    10621 cgccatattt ccccaccccg ccagccacga tcgccccccg caaggccgcc gcccgcgcca
    10681 gcagatccgg cgggaaagcg gcccttgcca gcaaccgctc cagcccgctt tcgcgcgccg
    10741 gatcgaaata cggccccagc tcgtccacca cgatcgacag cggcggcacg caatgcgccc
    10801 ccagccccgc cgagcggatc ggcccatcct cgatctcgcc cacagggccc gccagccgcg
    10861 ccagcacccg cgccggcgtg cgcgcctgcc acaccagcac ccggcccttg ccctgcccgc
    10921 cataaggtgg ccgcccccgg cccgcccaca gcagcgcatc cagcgtgcca cgcttccacc
    10981 gcgccacgcc atggatggcc cccaccgggc gattgccatc gatcagccgg cgccactgcc
    11041 ccagcagccg cgccgccgcc agcgcgccca tctccgcccc cgtgaacggg caggcatagc
    11101 gccagggcag gatcacctcg cgcgccagca ccgccagcag cccctgcggc ccttgcgcca
    11161 gcccggcata ggggcccgcc cccgccacct ccagcggccg gcccagcaag gcggccacca
    11221 gcgccagctc gcaagtggcg gg
//
LOCUS       DASRYE010000075         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_199063,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000075 DASRYE010000000
VERSION     DASRYE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199063"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1030)
                     /locus_tag="VFF98_04195"
     CDS             complement(77..1030)
                     /locus_tag="VFF98_04195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU62860.1"
                     /translation="MTDTAKADTLDLTLLLITHNEARHIERAIANVRPIAREVVVVDS
                     FSTDDTVERARAAGARVLQNPFVSHARQFNWGLDNAGITTGWVMRLDADEVLEPELIA
                     ELRATLPALPADVVGLNIDRKHIFMGRWIRHGGRFPLRLLRIWRLGHGRVEDRWMDEH
                     VVISGGRTLTLKGRFADWNLNDLTFFTDKHNKYATREAIERLNAKFGLFARPHEEAPA
                     DLPRQAQIKRWVKQTIYNRLPFWLGPLAYVLFRLTVQLGFLDGYQGVIYHVLQGFWYR
                     FLVGAKTVEFERELAGCPDNAARLARLAQLTGLPLAAAGQA"
     gene            complement(1178..1789)
                     /locus_tag="VFF98_04200"
     CDS             complement(1178..1789)
                     /locus_tag="VFF98_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62861.1"
                     /translation="MSRRPPASAPVPAPDRASEAVIARCRAEIESGLKQRLRRLLLPF
                     VRRRHKLAEVGEGFQWPRKGDMGLAPGSRIGRYAYLGAGFEAHGQVVVGDLCMISAGM
                     KIVGADHLYDEVGNPSRLAFPDVPRPITVFEADVFTGQRVTVIEGLRIGRGAVIGSGA
                     VVTKDVAPYTVVAGVPAREIRRRFTAEDEARHRAIVEPVAVSS"
     gene            complement(1793..2833)
                     /locus_tag="VFF98_04205"
     CDS             complement(1793..2833)
                     /locus_tag="VFF98_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU62862.1"
                     /translation="MDFTFYADPDPIDGIVALDSGLLKRFVPSACRYWRHLIWQPNAV
                     KVALERPGYDAIVYLASPYFLSTWVGAALARLRGIRVLFWGHGWLHAEPPGKAWLRRT
                     YFALANKMLVYAPRAVKLGARAGYPAERIKVVYNSLDVAKADAVIARIEDGSLQGVEP
                     AALFAHPDRPVVICTARLIDLCRFDLLIDAAAQLARRGRPVNVLLVGNGPERAALEAQ
                     AKALGVDVHFYGACYDEDVLGQLLYKADLTVSPGKIGLTAMHTLMYGTPALTHDNFDQ
                     QMPEVEALMADGVGALFSLEVPGDLADKIEAWFARPASRAETRALCREEIRRHWTPDV
                     QVRQIVAAIQEV"
     gene            complement(2940..4055)
                     /locus_tag="VFF98_04210"
     CDS             complement(2940..4055)
                     /locus_tag="VFF98_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU62863.1"
                     /translation="MSARFDIALYANGASHHVCDLLDALAGRPQVGAVTLITEAAVGE
                     DRLKLGWKGEIVGAYSQIEAPTAAEVEAFVAASGEDSFHIFVGMRHLRCVTEGISACI
                     RHGRRFAIMHETRVSEGLAGLARYVQSWLTEGRIRRHGIVLAIGAHGPKWFLRTGYRP
                     KQIFPFAYFIASHAPVAAAPRGQGGPVRIGYVGRLEDAKGLPEVLEMAALVQGPFELL
                     IVGGGTWAPACEELASRDSRVRYLGVLPVEQVQQFMSTLDVLVQPSRTVDDGWGVVVS
                     EALLNGVAVVASARVGASACLADPVRGRIVPPRRPQALADAIGDMAAGGAFSQEFRNL
                     RQQWALSHLTGDVGADYLLQIIGSLHSNVKRPKSFLL"
BASE COUNT          813 a         1492 c         1247 g          687 t
ORIGIN      
        1 ttggtcttgc tgtaatccaa tacgttcatt gcgcgccccc agtgccgctt taacgtgccg
       61 ctttaacggt gccgcttcag gcttgcccgg ccgcggccag cggcagcccg gtcaattgcg
      121 caagccgcgc aagccgcgcg gcattatccg ggcagccagc cagctcgcgc tcgaattcca
      181 ccgttttggc gcccaccagg aagcgatacc agaagccttg cagcacatgg tagatcaccc
      241 cctgatagcc atccagaaag cccagctgca ccgtcagccg gaacaataca taggccagcg
      301 gccccagcca gaacggcagg cggttgtaga tcgtctgctt cacccagcgc ttgatctgcg
      361 cctggcgcgg cagatcggcc ggcgcttcct catgcgggcg ggcgaacagg ccgaatttgg
      421 cattcagccg ctcgatcgcc tcgcgggtgg cgtatttgtt gtgcttgtcg gtgaagaagg
      481 tgagatcgtt gaggttccag tcggcaaagc gccccttcag ggtcagcgtg cgcccgccgc
      541 tgatcaccac atgctcatcc atccagcggt cttccacccg gccgtgcccc agccgccaga
      601 tgcgcagcag ccgcaaggga aagcgcccgc catggcggat ccagcggccc atgaagatgt
      661 gcttgcgatc gatgttgagg cccaccacat cggccggcag cgccggcagc gtggcgcgca
      721 gttcggcgat cagttccggc tccagcactt cgtcggcatc cagccgcatc acccagccgg
      781 tggtgatgcc ggcattgtcg aggccccagt tgaactggcg cgcatggctg acgaaggggt
      841 tttgcagcac gcgcgcgccg gcggcccggg cgcgctccac ggtatcgtcg gtggagaagc
      901 tgtccaccac caccacctcg cgggcgatgg ggcgcacatt ggcgatggcg cgctcgatat
      961 ggcgcgcctc gttatgggtg atgagcagca aggtcaggtc gagcgtgtcg gccttggcag
     1021 tatcggtcat ctgtactctt tcgatccgcg cgtcgcagcc cccaccgcca cgcctggtgc
     1081 ccgttgcccg cagcagcaac ggggcatggc cagcaaacaa cctgccacgg acaggaggcg
     1141 ccggccaagt ataattacat atttttattt gatgaaccta tgacgacacc gccaccggtt
     1201 cgacgatcgc gcgatggcgg gcctcgtcct cggcggtgaa gcggcggcgg atttcgcgcg
     1261 caggcacgcc tgccaccacc gtgtagggcg ccacatcttt cgtcaccacc gcgcccgagc
     1321 cgatcaccgc gccccgcccg atccgcaagc cctcgatcac cgtcacccgc tggccggtga
     1381 acacatcggc ctcgaacacg gtaattggcc gcggcacatc gggaaaggcc aggcgcgacg
     1441 gattgcccac ctcgtcatag agatgatcgg cgccgacgat cttcatgccg gccgagatca
     1501 tgcacagatc gcccaccacc acctgcccat gcgcctcgaa gcccgcgccc agataggcat
     1561 agcggccaat gcggctgccc ggtgccagcc ccatgtcgcc cttgcgcggc cactggaacc
     1621 cctcgcccac ctcggccagc ttgtggcggc ggcgcacaaa cggcagcagc agccggcgca
     1681 ggcgctgctt cagcccgctt tcgatctcgg cccggcagcg cgcgatcacc gcctcgctgg
     1741 cacgatcggg cgcgggcacg ggggcgctgg cgggcgggcg tctcgacacc tgtcaaacct
     1801 cctgaatggc ggccacgatc tggcgcacct gcacatcggg ggtccagtgc cggcggattt
     1861 cctcgcggca cagggcgcgg gtttcggcgc ggcttgccgg ccgggcaaac cacgcctcga
     1921 tcttgtcggc cagatcgccc ggcacctcca ggctgaacag cgcgcccacg ccatcggcca
     1981 tcagcgcttc cacctcgggc atctgctggt cgaaattgtc atgcgtcagc gcaggcgtgc
     2041 catacatcag cgtgtgcatc gccgtcaggc cgatcttgcc gggcgaaacg gtgagatcgg
     2101 ccttgtagag caattgcccc agcacatcct catcataaca ggcgccatag aaatgcacat
     2161 ccacgcccag cgccttggcc tgggcttcca gcgccgcgcg ctcgggccca ttgcccacca
     2221 gcagcacgtt gaccgggcgc ccgcgccggg ccagctgcgc tgcggcatcg atcagcaggt
     2281 cgaaacggca cagatcgatc agccgcgccg tgcagatcac caccggccga tccggatggg
     2341 caaacagcgc cgccggttcc accccctgca ggctgccatc ctcgatccgc gcgatcaccg
     2401 catcggcctt ggccacatcc aggctgttgt aaaccacctt gatccgctct gccggatagc
     2461 ccgcccgcgc ccccagcttc accgcccgcg gggcatagac cagcatcttg ttggccaggg
     2521 cgaaataggt gcggcgcagc caagccttgc cgggcggctc tgcatgcagc cagccatgcc
     2581 cccagaacag cacgcgaatg ccgcgcagcc gcgccagcgc cgcccccacc caggtggaca
     2641 ggaaataggg gctggcgaga tagacgatcg catcatagcc cggccgctcc agcgccacct
     2701 tcacggcatt gggctgccat atcaggtgcc gccaatagcg acaggccgaa ggcacgaagc
     2761 gcttcaacag ccccgaatca agcgcaacga tgccgtcgat cggatcgggg tctgcgtaaa
     2821 aagtaaaatc cacatccgca gatgcatcca gagcgcacat caccgcacgt cgataatgcg
     2881 gaaggaaatg gtaaacgatg gcaacccgca taggcatctc tattcgtgac gtaagcctct
     2941 cataagagaa agctttttgg tctttttaca ttactatgaa ggctgccaat gatttgcaat
     3001 agataatctg cgcccacatc tcctgtcaga tgtgacagcg cccattgctg gcgcagattg
     3061 cgaaattcct gcgaaaaagc gccgccggcg gccatatcgc cgatggcatc ggcaagcgcc
     3121 tgcgggcggc gcggcggcac aatccgccca cgcacggggt ctgccaggca ggcagaggcc
     3181 cccacccgcg cgctggccac aaccgccacg ccattcagca acgcctcgct gaccaccacg
     3241 ccccagccat cgtccaccgt gcgcgagggc tgcacaagca catccagcgt ggacatgaat
     3301 tgctgcacct gctcgaccgg cagcaccccc agatagcgca cgcgcgaatc gcgactggcc
     3361 agttcctcgc aggccggcgc ccacgtgccg ccccccacga tcagcaattc gaacggccct
     3421 tgcacaagcg ccgccatttc cagcacctcg ggcaggccct tggcatcctc cagccggccg
     3481 acatagccga tccgcaccgg cccaccctgc ccgcgcggcg ccgccgccac aggcgcatgg
     3541 gacgcgataa aatacgcaaa aggaaagatt tgtttcggcc gatagcccgt gcgcaaaaac
     3601 catttcgggc catgcgcgcc aatcgccagc acaatgccat gccggcggat gcgcccttcc
     3661 gttaaccaac tctgcacata gcgcgccagc cccgccagcc cctcgctcac gcgggtttca
     3721 tgcatgatgg caaagcggcg gccatggcgg atacaggccg atatcccctc ggtcacgcag
     3781 cgcagatgcc gcatgccgac aaagatatgg aaggaatcct cccccgaagc ggccacgaac
     3841 gcctccactt cggcagcggt tggcgcctcg atctggctat acgccccgac gatctcgccc
     3901 ttccagccca gcttcaggcg atcctcgccc accgcggcct cggtgatcag cgtgaccgcc
     3961 cccacctgcg gtcgccccgc cagcgcatcg agcagatcgc atacgtgatg cgaagcgcca
     4021 ttggcataca aggcgatatc aaagcgggca gacattattg acccctatac caataactat
     4081 tgagagcgcc tactagccat caatcatttt tgtattgata ttggtattgg tagtaattgt
     4141 agccgtagta gtatgagtat tggttgcctc tgcggagggg gtcgaacttg gtgaggacgc
     4201 cgccgacgac attggagttg tgggcgcgca ggcggcgca
//
LOCUS       DASRYE010000076        27385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_110729,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000076 DASRYE010000000
VERSION     DASRYE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 27385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27385
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110729"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFF98_04215"
     CDS             complement(<1..910)
                     /locus_tag="VFF98_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU62864.1"
                     /translation="MIGLLWRRAGQVTNSLVFRIGLLLALGLILASSAALGVAMQVHW
                     HDLLTMRNEQVVASSADIAQRLQRDPDHTEELLSTGRILGARDAPQSWQMSQPDEDLG
                     ELLRARLGPASAAQAMSMPHEQCFPYFDMRLKAAGTSDFALPDCWFVRFRDPVGHWRA
                     LSIDLPTIRHPNSFPNAIYISLLVLACASLAFLVTSLLAWPLRRLTEAAHTFSLSVQP
                     DAIPEVGPREVKSALVTFNLMQQRIRAGFTERTHMLGAIAHDLQTPLTRMRLRVEQID
                     EEALRGRLIADLSVMQRLVRDGLDLAR"
     gene            complement(907..1659)
                     /locus_tag="VFF98_04220"
     CDS             complement(907..1659)
                     /locus_tag="VFF98_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU62865.1"
                     /translation="MMLAPRDATAAAARILVAEDDADIRELIIAQLERETYEILPAAS
                     VAEVRAALAAAGTVGGRIDLIILDLNLPDGDGLDLCRALRADGYEGAIIMVTARDAAI
                     DRVLGLELGADDYLTKPFEPRELLARVRNWLRRLGGPAGAQPRNARKAHIGPWQVDLY
                     HRRLIAPDARLVMLSSAEFRLLQRFLDTPNQVLSREELLPERGATVAFDRSIDLQIHR
                     LRQKLASEPGGDDLILTVRNEGYVLAGEVVFE"
     gene            1885..3054
                     /locus_tag="VFF98_04225"
     CDS             1885..3054
                     /locus_tag="VFF98_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU62866.1"
                     /translation="MTPARPTFSPLSPAGLAPIGLGLAASLALVLAVAGCSGAPPQQA
                     GPTQVGVITLAAQPLTLSNELTGRTSAVAIADVRPQVDGVIRDRLFAEGSLVRAGQPL
                     YQIDARLYKASLDTAAAQLESAQATLADADAKAKRYRALPDPQAVSAQDMDDTLATAR
                     TARASVHQYSATVETARVNLEYTRVLAPITGRIGRSTYTKGALVSASQTTALATITQL
                     DPIYVDITQSAAEWLRLRQALAKGSILPTSTSATLKLEDGTTYGRPGSIEFTEVAVDE
                     SAGTVTLRARFPNPDGLLLPGMFVRVATPQGNLPRAILAPQQGITRDAKGNATALVVN
                     RAGKVEQRNVVADRAIGTSWLVTSGLAAGDQLIVEGTSKVHAGDPVHATQVKLGQ"
     gene            3059..6226
                     /locus_tag="VFF98_04230"
     CDS             3059..6226
                     /locus_tag="VFF98_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU62867.1"
                     /translation="MGISKFFVGRPIFAWVIAIAIMLAGIGSILSLPVAQYPDVAPPA
                     VTITATYPGANAQTLDSSVTQVIEQQLTGIDNLLYFSSSSSSAGTATITATFQKGTDP
                     DIAQVQVQNKVQQANSRLPTEVQQQGLVVTKSNSDFLMVVGLYDETDKATDADIADYL
                     VSNLQDDIARVNGVGQVQVFGSQYAMRIWLDPEKLAARQLIPSDVTSAITAQNVDLSA
                     GQIGARPAVAGQQITAVVQAKSRLQTPDQFRAIVVKTLTGGSVVRLGDVARVEMGQEN
                     YTMSARLSGHPAAGLALQLAPGADALKTAAAVKTEVARLTTHLPAGYQVTFARDSTDF
                     VKLSIREVIETLVIAVVLVVVVMYLFLQSWRATLVPAIAVPVVLLGTFGVLALFGYSI
                     NTLTLFGMVLAIGLLVDDAIVVVENVERIMAEEGLPPREATIKSMGEIGGALVGIALV
                     LSAVLLPMAFFGGSTGVIYRQFSVTIVAAMALSVVVALVLSPALTATMLKAGDHDPLE
                     RPGLFGRFNRWFEDMTGRYVNATGKVIARRALHFGVFGAITLVLGLLFWRLPTGFLPE
                     EDQGIAMVVFSLPPGATEERSAKVRQAITDHFLQNEQNSVRKVMAVTGFSFFGTGQNT
                     GMAFIDLLPFEERGKASQSASAVVGRAFGAFASLRDALVIPLTPPAISGLGQSNGFSF
                     ELRNSGNLSQTEFAALRDQLLDAARKDPKLAQVRSGLLPDAPQLRVTLDDTKLNVLGV
                     AESDATSTLSTAWGGTYVDDFIDRGRVKKVYVEGDAGARMTPEDLARWQVRGTTSTTS
                     ISAMVPFSAISTTSWEKGPDTLSRFNGLPAYEIDGQAASGYSSGDAMREMEKLQAQLP
                     AGTSYAWSGLSFQENQASGQAMGLYAISVLVVFLCLAALYESWSVPLAVLLVIPLGVI
                     GAVLAVTLRGLDNNIYFQVGLLTTIGLSAKNAILIVEFAEAARRRGVPVLEAALNGAK
                     VRLRPILMTSLAFIAGVFPLAVATGAGAQSRIAIGTAVLGGMFTATALAIFYVPMFFL
                     AVAQLFHPAAPAASDPAASDHEDHA"
     gene            6226..7605
                     /locus_tag="VFF98_04235"
     CDS             6226..7605
                     /locus_tag="VFF98_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU62868.1"
                     /translation="MRRALLITAALLPLAGCNLAPHAATPALPTPQQWPQGAAYGPLE
                     EGSPALPWRQVAGDARLVKVIEMALSSSRSLREQVAALAEARAQYHIQRAALLPTVDA
                     SASGSVTRGIANQANNADSYSVSAGASSFEIDLFGRLRNEAKASFESYLASQSGLKST
                     RLSLVSDVATAWLTYAADKDTLTAAEGTLDSARKTLELERGLFKSGLASGSDVASAIA
                     TVEGAASDVAADITTLAQDRNALDLLVGAHVADELLPDTLDGLDHAISAPPAGLPSEV
                     LRQRPDVVEAEHTLKGTYATIGAARAAFFPAITLTSSVGLASTALSQLFTHGAAQWNV
                     APSASVPLLGGTTGANLAYAKAQRDYALAAYELAVQTAFRDVANALARRGTIADQRAA
                     QDKQMLAYRRAWELADGQRRAGTGTYLAALVAQRSYYSAQQTRITAQLADLSNRFTLY
                     AAVGADPSL"
     gene            complement(7609..8841)
                     /locus_tag="VFF98_04240"
     CDS             complement(7609..8841)
                     /locus_tag="VFF98_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU62869.1"
                     /translation="MSTPATAALDAALRRIAATDREIRAWTHLAPAAARAAAAAQDRG
                     EGPTGPLAGIPIGIKDVIDVAAMPTSHNSPLGLAAPAIADAPCVATLRAAGALILGKT
                     DTTEFAAAGRNAATANPHDGARTPGGSSSGSAAAVAAGHVPLALATQTGGSTIRPASF
                     CGIHALKPTWGQISREGAKLYAASLDTIGLYASSLAGLDLLAETYDFAPPPPPRASLR
                     IGLCRTPYWPRAEAETQAAMADAAARLAAAGAVVEEVALPAACDGLEEAFMAILFREG
                     AAAFLNLARAEPALLHADFHQRVAARARYPDEALRAAYDLTVTARIAVEAAMAGFDAF
                     LAPSAPGVAPLGRGPGNPIFNQMWTQLHLPVVHLPLYRAQGGLPLGLSLVAPRFADRA
                     LLAAAARVEALLGRDRPL"
     gene            8929..10125
                     /locus_tag="VFF98_04245"
     CDS             8929..10125
                     /locus_tag="VFF98_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62870.1"
                     /translation="MSSPSHSLKQALISTTLVQVLATAIALALTPIAPRAAHDFGIDP
                     HYIGFQISLIYCAGAFASAASGTLIHRHGAVAMEVLSLALFGGGLLLLATARLPLGML
                     GSVLIGVGYGLQNPASSQLLNQACPPEKRSVVFSIKQAGVPLGALLASLALPWLDGLI
                     GWRLALVIGAIGPVALILHILRAHRHEAHRRRPGGAFLPGLIEEQRLVSREPRLRVLA
                     LLGLLYSAVQLSLSAYIVLMLVDNRGWPLVSAAAMGGLLQGFGALGRVFWGWLADRTR
                     AGLAILALIGLGSSAALVALPLLGALPAALQGVLLCALGFFLSGWNGVAMAEIARSAP
                     LAQTGRVMGGALVYTFLGVMLGPASFAFLYEALGRYDSTFAAVALATTLGALAAAWRH
                     LAERHA"
     gene            complement(10216..11295)
                     /gene="rutA"
                     /locus_tag="VFF98_04250"
     CDS             complement(10216..11295)
                     /gene="rutA"
                     /locus_tag="VFF98_04250"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HZU62871.1"
                     /translation="MDLGVFVPIGNNGWLISKNSPQYMPSFELNKAIVQKAEGYGFDF
                     ALSMIKLRGFGGETRFWDYNLESFTLMAGLAAVTTKIKLFATTAVLTLPPAIAARMAI
                     TIDSIAPGRFGLNIVSGWAKGEYAQMNLWPGDDYFGYRYDYSEEYVRVMQELWEQGSS
                     DFKGQYFQMDDCKLGPQPSGKIEIVGAGQSERGMDFVATYGDYNFVTCGGINKPFQHQ
                     PTVEKLVEASQRSGRDVGAYILCMVIMGETDEEAFAKWEHYKAGADMGALAWMADQGS
                     KDTTGDANSTSRHINLPEGAVNFNIGTFIGSHATVARLLDEAASIPGTKGIMLTFDEF
                     LTGLDEFATKVQPLMQCRAHIAEPA"
     gene            complement(11313..12707)
                     /locus_tag="VFF98_04255"
     CDS             complement(11313..12707)
                     /locus_tag="VFF98_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU62872.1"
                     /translation="MSAAQALASLDLVAQAEAIAEGAFTAEALTEAALAAAHARADLN
                     AFVAIAGAPALEAARAVDLARARGQVLGPLAGVPLAHKDMFYRQGRVSGCGSKIRAGF
                     VAGETCPLLARLDAAGAIDIGRLHMAEFAMGPTGHNAHLGRCRNPWAGAAITGGSSSG
                     SGAAVGARIVAGALGSDTGGSVRLPAAICGVVGLKPTHGRLGQQGMMGLSESMDTAGP
                     LARSARSVARLMTVIAGDGEDFAQGLRGDLAGLTIGLPVAYYADGLAAEVAAAFAEAV
                     RVLQGLGARVIEVAMPDQAHLAAMAGLVWAPEAAALHLDWLRDRPQDYGEQVRNRLLH
                     GLAMPAATYVRARRLRSADLAAMLAGPLARCDILATPTLRMPTPGGEAAGANAGAAMD
                     KVVGDLSALTRPISYLGLPALSTPMGFDGAGLPLGLQLIGAPRAESLLLGAAHAFERA
                     TDWLARPLPPLPST"
     gene            complement(12704..12913)
                     /locus_tag="VFF98_04260"
     CDS             complement(12704..12913)
                     /locus_tag="VFF98_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62873.1"
                     /translation="MSAARQWLAAALGLADSAITPEWEARCLAHWQADGAVLARGVAA
                     LGAEAFFDCPAASAEALLAGKARQS"
     gene            complement(13139..14149)
                     /locus_tag="VFF98_04265"
     CDS             complement(13139..14149)
                     /locus_tag="VFF98_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU62874.1"
                     /translation="MANIKDIAAKAGVSIATVSRALRQPNLVKEKTAQRIEAAIRDLD
                     YKPNMLAASLRRQKADAVIIAVPDIANPFTAAFVQGVENVARDSGTKVLLAITEGRED
                     LLDSHYEMVAGKQADGMILLDLQLPGVLRDRPADAAPLPIVLACEYQDGSNQPRVRVN
                     DLEAAADAAMHIAQLGHTRIACISGPANQRMSRDRQRGFRLGLRRFGLDLPEPMVAGG
                     DYSLLSGVEGARCLLDRGEPFTALLCENDEMALGAIHAIAARGLRVPQDISVIGIDDF
                     RFSEFSNPALTTVALPTTLIGEQAMRLMLDFGLDADGACREVILPHQLVVRESTARPT
                     EG"
     gene            14558..16672
                     /locus_tag="VFF98_04270"
     CDS             14558..16672
                     /locus_tag="VFF98_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62875.1"
                     /translation="MLHKWSLAAGVALASIMPALAHAETPAASAQASSGLGEIVVTAE
                     KRESTASKTPIAMTVASGADLIRAGVADVNGLSNIAPTLNIAQNNQNTMITIRGISSR
                     DYTETGNPAVAVSIDNFFLQNGTALNVGFYDLDRVEVLRGPQGTLYGRNASAGAINIQ
                     TAKPTKKLEGSVSLGYGYKNALLVEGMINLPVTDNLQVRAAFSVHNRDGYRNNGSLST
                     GNENVSKGGNDDVSQSARLHVMWEPTSNFTALVTGEYTHVGGVGPVIKGILADQVNGD
                     GTLKIGSTTNWQLSNQSYIDITQKTIRGTLTYDLGFAKLTYIGGYRQQHILHGNDQDG
                     GTTYSYAFPTDGKTDTENHELRIASNGNTRFGWQGGLYYFQDGGQALTYFQVLGNPVY
                     NYYTFNYLTNAQSFAAYGQVYYKIFDNLKLSGGARYTEEYKNQLGYSIIAGTYTDVNW
                     KYHGGKDTYHVGLDWQVDPHHLVYAKFDTGFKTGGVQNLRSYGPESIKAYEVGAKNRL
                     FGNTVELNIDAFYYDYSQLQVQQNDPETAISYIYNAGKARDYGVEFEGRWAITPNDRL
                     DANINYVNARYTDFVNTGVQYAGHALPQSPNWALGGGFSHDFELKTGAKVTARVQTRY
                     ESKSYFGFENANYELQKAYTKTDLILSYTPLGKGFSASAFIYNLENSTILTDSEAAGY
                     AGGYLVQFADPRTYGVRLNYSF"
     gene            complement(16797..17939)
                     /gene="ispG"
                     /locus_tag="VFF98_04275"
                     /gene_synonym="gcpE"
     CDS             complement(16797..17939)
                     /gene="ispG"
                     /locus_tag="VFF98_04275"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZU62876.1"
                     /translation="MSDHNPGLRPWRDIARRKSRQIMVGKVAVGGDAPISVQTMTNTL
                     TSDARATIDQIRRCEEAGADIIRVSCPDVESTTAFREIARAAQVPLVADIHFHYKRAL
                     EAADAGAACLRINPGNIGSADRVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPC
                     PEALVESALDHIKLLQDHDFHDYKVAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAG
                     GLIGGTVKSAIGIGNLLWAGIGDTLRVSLSAPPEEEVRVGFEILKSLGLRTRGVRVVS
                     CPSCARQGFDVVKTVSALEDRLQHIKTPISLSVLGCVVNGPGEARETDIGITGGGNGK
                     HMVYLSGVTDHHVQSEDMLDHIVRLVEAKAAEIDAANAAKEAEAAE"
     gene            complement(18165..19052)
                     /locus_tag="VFF98_04280"
     CDS             complement(18165..19052)
                     /locus_tag="VFF98_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZU62877.1"
                     /translation="MTPSDAAERLLQTAEGVTLRLRLAGRGTRLGALLLDLALQGLVL
                     GALALALLGIARGLGGGKAAGGALQALFVLWLLASFVLRNAWFLGFELGPRGATPGKR
                     MLGLRIATRDGSRLTADRVLARNLLREVELSAPLAVPGLLGTSGGAHGLGWSMAAGLA
                     WLVLFAGLPLFNRDRLRPGDIVAGTWVVEAPHRRLAAALSVPQGPAGGPYRFSAAELA
                     AYGEYELGVLEKVLREGAPAPMAAVAGAIAARIGWHPPADAEEIHAFLLAYYTQLRGR
                     LEAGMQLGHRRASKHAPPR"
     gene            complement(19049..20107)
                     /locus_tag="VFF98_04285"
     CDS             complement(19049..20107)
                     /locus_tag="VFF98_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZU62878.1"
                     /translation="MKAFWRDLAARWRAGAPAVPEGWQASLGSDRFRLAREADWARLE
                     RIVGLLEKGRPRAIADADLLALPGLYRQAASALAVARGTMLDAAVQAYLEALVQRAWF
                     QLYGPNIGLGGWLAGFLAGGWPRAVRALAVDIAICLIVLALGTAVGWLLCAADPQWFG
                     ALVGDMAQGRNPAASPAALRATLFGHTQESGLSVLAAYLFGHNAQIALLEFALGFVVG
                     VPSLLLLVQNAALLGAFLWLFAGAGLLPEAAGWLAVHGTTELFALVLAAAAGLHMGRA
                     VAFPGRAGVLEAVSAAGRRAALVMLGVVAMLFVAGLLEGFARQLIEPTAARMALGGLM
                     LVLWLAYFGLVGRGGEAP"
     gene            complement(20104..21465)
                     /locus_tag="VFF98_04290"
     CDS             complement(20104..21465)
                     /locus_tag="VFF98_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZU62879.1"
                     /translation="MALRALFPSRPSRPGRPALLPTARAVLLLALLAPAALLLAAVRA
                     DLWQLAPALGLAMGLLVLADAALAAGASADWRIDMAGEAEVGGALPIALDVAGGGPAG
                     GIAAALAVGPLLAPGGVLRLALRRDGSGRARGQALCHPARRGLAEVESLALRRTGPLG
                     LGACQRRWPVARALRILPSLAVVRSPALATYLKDARAGEIARRLRGEGSQFETMAEFQ
                     PGMDRRRIDWKVSARHGRLMAREYEAERNNQIVFAFDCGGAMCEDLGRLTRLDRAIAA
                     ALAAGWVALREGDRVGLFGFAAQVELATPLYGQGGFARLRAAAAELAYRAEEPNFTLA
                     LASLVAHLRRRSLVVVFSDFTDPTSATMMVESIGRLLARHRVLFVTLEDAGLEEMARG
                     APVDLGALARAVGAQGLLDERARVLARLRQAGVEVLEAPAGQLDMRLIDTYLGLRARG
                     GVA"
     gene            complement(21476..22450)
                     /locus_tag="VFF98_04295"
     CDS             complement(21476..22450)
                     /locus_tag="VFF98_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZU62880.1"
                     /translation="MSDAEHLPLAALTELAGAIRAETARAVVGQADVVDHLLIALLAR
                     GHVLLEGPPGTAKTFLARCFAAASGLDFGRIQFTPDLMPGDILGANLFNFQTSQFTLT
                     RGPIFCELLLADEINRTPPKTQAALLEAMQERGVTLDGQRHALPDRFLVVATQNPVES
                     QGTYPLPEAQLDRFLFKLALPYPALEEEVRIVSRFGAGTGAESPADHGIVPVADGARL
                     AAAARALAGVQLAAPVADYIARLIRATRQSADIAVGASPRAGVLMAQAARARAALDGR
                     DYVLPDDVKALAPAVLRHRLTLSPAAEIEGKAAEALVAGFIEATEAPR"
     gene            complement(22447..23679)
                     /locus_tag="VFF98_04300"
     CDS             complement(22447..23679)
                     /locus_tag="VFF98_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZU62881.1"
                     /translation="MSGQGPFSGKAALALVAAMGALLLGLAWLAGAAPAQVPAAGAHA
                     GGRGLDGYAGLATLLEVAGYPVAVGSSAGFGASEGSGLLVLTPPAEADGRAIQRRVMA
                     HLPHGPVLVVLPKWQTAAAQGASGAAPGWVWLTGLALPGWKGFADDVAIGVAPAAPPV
                     IGPEGTGAVLPRADLALAGTGAALAPLVTDGAGRVLAAAYRPGWVPPRAPPLILVFEP
                     DLLNNAGLAHAENAALAEALVRRALAGRQGAARAIGFDLALNGLGRPTNLVALAFTPP
                     FLAATLCLLLAGLGVGWRGFVRLAPPERALAAPANGRRLLVANMAALILRARRWRVLG
                     APYADGAAARLARALGLARSLPPPAQQAALARLLAARGRPDLDLPATLEALAAPHGEG
                     ALLQAAQQLHTIERTLTR"
     gene            complement(23676..24380)
                     /locus_tag="VFF98_04305"
     CDS             complement(23676..24380)
                     /locus_tag="VFF98_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62882.1"
                     /translation="MRDAIATSAATSAAMARPDPAALAHWRALRAHGEIQFAPLPPPA
                     PYKPPFWLEWLVKALEWLARLFARLFGPAGHALGGAWPWMASLAVVLGAGLVLAAGWW
                     LVRRWPARRRHALADKAAPWAPDAAEAAALLADAESLAAAGAFDEAVHLLLARSVAQI
                     AAARPGLLHPATTARELAGQPRLPAAARMAFGLIAARVEQCRYALRPLALADWQAARA
                     AYADFAQVPLAEGAAR"
     gene            complement(24494..25393)
                     /locus_tag="VFF98_04310"
     CDS             complement(24494..25393)
                     /locus_tag="VFF98_04310"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZU62883.1"
                     /translation="MHSFAEPALIQVKTLTDLAETDPARAVAFQGAPGCNSHRAALEY
                     DPACLPLPCFSFEDALDAVKEGRADRAMIPIENSQHGRVADIHFLLPESGLAIVGEHF
                     LAIEHALMALPGARGPFAAAYSHPQALGQSRHYLRARGITPLAYADTAGAAAFIAEQG
                     DPAAAAIAPALAAQLYGLDIVEHNVEDAADNTTRFVVLARAPRDPAELAGQVTMTTLI
                     FEVRNIPAALYKALGGFATNGVNMTKLESYQKGASFAATTFYADIVGRPGDPAVDRAI
                     EEAAFHCKNLRLLGSYPQARPRG"
     gene            25512..26123
                     /locus_tag="VFF98_04315"
     CDS             25512..26123
                     /locus_tag="VFF98_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HZU62884.1"
                     /translation="MTGRFNTIAGWVLGSGIVALGLASLSAHYFSADKPERPETMGYA
                     IEGVAGGAKGPAEVPLEVLLAKADPAKGQEVFQKCASCHTITQGGANGIGPNLYGILG
                     DAIAQGRGGFAFSDGLKKHSGKWDFKELNTWLTNPRAYADGTKMTFAGLDDAADRANV
                     LAYLNKQGSNLPLPAPKADAKPAAGDAKPAAGDAAPAASSAAK"
     gene            complement(26182..26859)
                     /locus_tag="VFF98_04320"
     CDS             complement(26182..26859)
                     /locus_tag="VFF98_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZU62885.1"
                     /translation="MTRGVKDLGQRLKSAKKRTASSARWLQRQLNDPYVKKARADGYR
                     SRAAYKLIELDDKYALLKGARRVVDLGVAPGGWTQVVRARAPQARVVGIDLLPTDPID
                     GAILFQMDFMADEAPDALMDALGGLPDLVLSDMAANTVGHKQTDHLRTMGLVETAADF
                     AIQNLGKGGAFVAKVFAGGTDPGLLAILKQHFTTVKHAKPPASRKDSSEWYVIAQGFK
                     GRAAPAD"
     gene            complement(26856..>27385)
                     /locus_tag="VFF98_04325"
     CDS             complement(26856..>27385)
                     /locus_tag="VFF98_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HZU62886.1"
                     /translation="RDWVSVPWGVVSLYETCTGPEDTREQRLDRYTRMRAMVAESLAG
                     FAGRVAPWRAHERPLRLLGTSGTVTTLASLHLDLPQYDRRAVDGAIVPAPAMRAISER
                     LSGMSVAERRGLPCIGRERADLVVAGCAILDAILDLWPAERLGVADRGIREGILRGLI
                     NVDGRPGAAGEGEKQ"
BASE COUNT         4475 a         9734 c         9107 g         4069 t
ORIGIN      
        1 cgcgggccag atccagccca tcgcgcacca gccgctgcat caccgacaga tcggcgatca
       61 gccggccgcg cagcgcctcc tcgtcgatct gctccaccct gaggcgcatg cgcgtcagcg
      121 gcgtctgcag atcatgggcg atggcgccca gcatgtgggt gcgctcggta aagccggcgc
      181 ggatgcgctg ctgcatcagg ttgaaggtga ccagcgcgct cttcacctcg cgcgggccca
      241 cctcggggat ggcatcgggc tgcaccgaaa gcgagaaggt gtgggcggcc tcggtcaacc
      301 ggcgcagcgg ccaggccagc aggctggtga caaggaaggc caggctggcg caggccaaca
      361 ccagcaggct gatgtagatg gcattgggga aggaattggg gtggcggatg gtcggcaggt
      421 cgatcgacag cgcccgccag tggcccaccg gatcgcgaaa gcgcacgaac cagcaatcgg
      481 gcagggcaaa atcggacgtg ccggccgcct tcagccgcat gtcgaaatag gggaagcact
      541 gttcatgcgg catcgacatc gcctgcgcgg cagaagccgg ccccagccgc gcgcgcagca
      601 attcgcccag atcctcgtcg ggctggctca tctgccagct ttgcggcgca tcgcgcgcgc
      661 ccaggatgcg gccggtggac agcagttcct cggtgtggtc gggatcgcgc tgcaggcgct
      721 gggcgatatc ggcgctgctg gccaccacct gctcgttgcg catcgtcagc agatcgtgcc
      781 agtgcacctg catggccacc cccagcgccg ccgaggaggc caggatcagc cccagcgcca
      841 gcagcaggcc aatgcggaac accagcgagt tggtgacctg cccggcccgg cgccacaaca
      901 ggccgatcat tcgaacacca cctcgccggc cagcacatag ccttcgttgc gcacggtgag
      961 gatgaggtcg tcgccgcccg gctcgctggc cagcttctgg cgcaggcggt ggatctgcag
     1021 gtcgatcgag cggtcgaagg ccacggtggc gccgcgctcg ggcagcagct cctcgcggct
     1081 gagcacctgg ttgggcgtgt cgagaaagcg ctgcagcagg cggaattcgg ccgaggagag
     1141 catcaccagc cgggcatccg gggcgatcag ccggcggtga tacaaatcca cctgccaggg
     1201 gccgatatgc gccttgcggg cattgcgcgg ctgggcccct gccggcccgc ccaggcggcg
     1261 cagccagttg cgcacgcggg ccagcagctc gcgcggctcg aacggcttgg tgagatagtc
     1321 gtcggcgccc agttccagcc ccagcacccg gtcgatcgcg gcatcgcgcg cggtcaccat
     1381 gatgatcgcg ccttcatagc catcggcgcg cagggcccgg cacagatcca gcccgtcgcc
     1441 atcgggcagg ttcagatcga ggatgatgag atcgatccgc ccgcccaccg tgcccgccgc
     1501 cgccagcgcc gcgcgcactt cggccacaga ggcggccggc aggatctcgt aggtctcgcg
     1561 ctccagctgg gcgatgatca gctcgcgaat atcggcgtca tcctcggcaa cgagaatgcg
     1621 ggcggcagcc gctgtggcat cgcgaggggc taacatcatc tccggaactt ggccttcatg
     1681 ggaaccttgg cgcgtgagta tagggcccgg gcgaggccct gtctgcgccc tgaaacgcaa
     1741 tgaaacatga aactacgcag ccgaaccttg caatacactc tggtgctggc aaaccctgtc
     1801 tgttcgacgc atgcagcaat tcgagaagcg tctgccagca ggccggatgc gatctctgat
     1861 tcgcttgacg accaaggtag cccgatgacc cctgcgcgcc ccactttctc tcccctttcg
     1921 cccgccggcc tggcgcccat tggccttggc ctggccgcca gcctggcgct ggtgctggct
     1981 gttgcgggct gttccggcgc gccgccgcag caggcggggc ccacgcaggt gggggtcatc
     2041 accctggccg cccagccgct caccctgtcg aacgagctga ccggccgcac ctcggccgtg
     2101 gccatcgccg atgtgcgccc ccaggtcgat ggcgtcatcc gcgaccggct gtttgccgaa
     2161 ggctcgctgg tgcgtgccgg ccagccgctc taccagatcg acgcgcggct ctataaggcc
     2221 tcgctcgaca ccgccgctgc ccagctggaa agcgcgcagg caacgctggc cgatgccgat
     2281 gccaaggcga agcgctatcg cgccctgccc gatccgcagg ccgtttccgc ccaggacatg
     2341 gacgacacgc tggccaccgc ccgcaccgcg cgcgccagcg tgcaccaata cagcgccact
     2401 gtggaaaccg cgcgggtgaa cctggaatat acccgcgtgc tggcgcccat caccgggcgc
     2461 atcgggcgct ccacctatac caagggcgcg ctggtttcgg ccagccagac caccgcgctg
     2521 gccaccatca cccagctcga tccgatctat gtggatatca cccaaagcgc ggccgaatgg
     2581 ctgcgcctgc gccaggcact ggccaaggga tcgatcctgc ccaccagcac cagcgccacg
     2641 ctgaagctgg aggatggcac gacctatggc cgccccggca gcatcgaatt taccgaagtg
     2701 gcggtggatg aaagcgccgg cacggtaacc ttgcgcgccc gcttccccaa ccccgacggg
     2761 ctgctgctgc ccggcatgtt cgtgcgggtg gcaacgccgc agggcaatct gccgcgcgcc
     2821 attctggccc cccagcaggg catcacccgc gatgccaagg gcaatgccac cgccctggtg
     2881 gtgaaccgcg cgggcaaggt ggaacagcgc aatgtggtgg ccgatcgggc cattggcacc
     2941 agctggctgg tcacttccgg ccttgccgcc ggcgaccagc tgatcgtcga gggcaccagc
     3001 aaggtgcacg cgggcgatcc cgtgcatgcc acgcaagtga aactgggcca gtaaacccat
     3061 gggtatcagc aagttcttcg ttggccggcc gatcttcgcc tgggtcatcg cgattgccat
     3121 catgctggcg ggcatcggct cgatcctgtc gctgccggtg gcgcaatatc ccgatgtggc
     3181 gccgcctgcc gtcaccatca ccgccaccta tcccggcgcc aatgcgcaaa cgctcgacag
     3241 ttcggtgacg caggtgatcg agcagcagct caccggcatc gacaatctgc tctatttctc
     3301 ctccagctcg tcgagcgcgg gcacggccac catcaccgcc accttccaga agggcaccga
     3361 ccccgacatc gcccaggtgc aggtgcagaa caaggtgcag caggccaatt cgcgcctgcc
     3421 caccgaagtg cagcagcagg gccttgtcgt caccaagtcg aacagcgact tcctgatggt
     3481 ggtgggcctc tacgacgaga ccgacaaggc caccgatgcc gacatcgccg actatctcgt
     3541 ctccaacctg caggatgata tcgcccgtgt gaacggcgtg ggccaggtgc aggtgtttgg
     3601 cagccaatat gccatgcgca tctggctgga tccggaaaag ctggccgccc gccagctcat
     3661 cccctccgat gtgaccagcg ccatcaccgc gcagaacgtc gacctgtcgg ccggccagat
     3721 cggcgcgcgc ccggctgtgg ccggccagca gatcaccgcc gtggtgcagg ccaaatcgcg
     3781 cctgcaaacc cccgatcagt tccgcgccat cgtggtgaaa acgctgaccg gcggctctgt
     3841 ggtgcggctg ggcgatgtgg cccgggtgga aatgggccag gaaaactaca cgatgagcgc
     3901 gcgcctgtcg ggccatccgg ccgcgggcct ggcgctgcag ctggcgcccg gcgccgacgc
     3961 gctgaagacc gccgccgccg tgaaaaccga agtggcccgc ctgaccaccc acctgcctgc
     4021 cggctatcag gtgacattcg cccgcgattc caccgatttc gtcaagctgt cgatccgcga
     4081 ggtgatcgag acgctggtga tcgcggtggt gctggtcgtc gtcgtcatgt atctcttcct
     4141 gcagagctgg cgggccacgc tggtgccggc aattgccgtg ccggtggtgc ttttgggcac
     4201 gtttggcgtg ctggcgctgt ttggctattc gatcaacacg ctcaccctgt tcggcatggt
     4261 gctggcgatc ggcctcctgg ttgatgacgc catcgtggtg gtggagaatg tcgagcggat
     4321 catggccgag gaaggcctgc ccccgcgtga ggccaccatc aaatccatgg gcgagatcgg
     4381 cggcgcgctg gtgggcattg ccctggtgct gtcggcggtg ctgctgccga tggctttctt
     4441 cggcggctcc accggggtga tctaccgcca gttctcggtg acgatcgtgg cggcgatggc
     4501 gctgtcggtg gtggtggcgc tggtgctgtc gccggcgctc acggccacca tgctgaaggc
     4561 gggcgaccat gatccgctgg agcggccggg cctgttcggc cgcttcaacc gctggttcga
     4621 ggacatgacc ggccgctatg tcaacgccac cggcaaggtg atcgcccgca gggcgctgca
     4681 tttcggcgtg tttggcgcca tcaccctggt gctgggcctg ttgttctggc gcctgcccac
     4741 cggcttcctg cccgaggaag accagggcat cgccatggtg gtgttcagcc tgccgccggg
     4801 tgccacggaa gagcgttccg ccaaggtgcg ccaggcgatc accgaccatt tcctgcagaa
     4861 cgagcaaaac tctgtgcgca aggtgatggc ggtgaccggc ttcagcttct ttggcaccgg
     4921 gcagaacacc ggcatggcct tcatcgacct gctgcccttc gaggagcgcg gcaaggcctc
     4981 gcaaagcgcc agtgcggtgg tgggccgggc gtttggcgcc tttgccagcc tgcgcgatgc
     5041 gctggtcatc ccgctgacgc cgccggcgat ctcgggcctg ggccagtcga acggcttctc
     5101 cttcgagctg cgcaattcgg gcaatctgtc gcagacggaa tttgccgcgc tgcgcgacca
     5161 gctgctggat gccgcccgca aggatccgaa actggcgcag gtgcgctcgg gcctgctgcc
     5221 cgatgccccg cagctgcgcg tgacgctcga cgataccaag ctcaacgtgc tgggcgtggc
     5281 cgaaagcgat gccacctcca ccctctccac cgcctggggc ggcacctatg tcgacgattt
     5341 catcgaccgc ggccgcgtga agaaggtcta tgtggaaggc gatgccggcg cccgcatgac
     5401 gccggaagac ctcgcccgct ggcaggtgcg cggcaccact tccaccacca gcatcagcgc
     5461 catggtgccc ttctcggcaa tttccaccac cagctgggaa aagggccccg acacgctgag
     5521 ccgcttcaac ggcctgcccg cctatgaaat cgatggccag gcggccagcg gctattcctc
     5581 gggcgatgcc atgcgcgaga tggaaaagct gcaggcgcaa ttgcccgccg gcacctccta
     5641 tgcctggagc ggcctctcct tccaggaaaa ccaagccagc gggcaagcga tgggcctcta
     5701 tgcgatctcg gtgctggtgg tgttcctgtg cctggccgcg ctttacgaaa gctggagcgt
     5761 gccactggcc gtgctgctgg tgatcccgct gggggtgatc ggcgcggtgc tggcggtgac
     5821 cttgcgcggg ctggacaaca acatctactt ccaggtcggc ctgctcacca ccattggcct
     5881 ttccgccaag aacgccatcc tcatcgtgga atttgccgaa gcggcccgcc ggcgcggcgt
     5941 gccggtgctg gaagcggcgc tgaacggcgc caaggtgcgc ctgcggccca tcctgatgac
     6001 ctcgctggcc ttcattgccg gcgtgttccc gctggcggtg gccacaggcg ctggcgcgca
     6061 aagccgcatt gccatcggca cggcggtgct gggcggcatg ttcaccgcca ccgcactggc
     6121 catcttctac gtgccgatgt tcttcctggc ggtggcccag ctgttccatc ccgccgcccc
     6181 cgctgcttct gaccccgctg cttctgacca cgaggaccac gcctgatgcg ccgcgccctt
     6241 ctgataaccg cagcgctgct gccgctggcc ggttgcaacc tggcgcccca cgccgccacg
     6301 cctgccctgc ccacgcccca gcaatggccg caaggcgccg cctatggccc gctggaagag
     6361 ggcagcccgg cgctgccctg gcggcaggtg gcgggcgatg cgcggctggt gaaggtgatc
     6421 gagatggcgc tgtcctccag ccggtcgctg cgcgagcagg tggccgccct tgccgaggcg
     6481 cgcgcgcaat atcacatcca gcgcgccgcc ctgctgccca cggtggatgc ctcggcttcg
     6541 ggctctgtca cccgcggcat tgccaaccag gccaacaatg ccgacagcta cagcgtctcg
     6601 gccggcgcca gcagcttcga gatcgacctg ttcggccgcc tgcgcaacga ggccaaggcc
     6661 agcttcgaga gctatctggc cagccagtcg ggcctcaaat ccacgcgcct ctcgctggtt
     6721 tccgatgtcg ccacggcctg gctcacctat gccgccgaca aggacacgct gaccgccgcc
     6781 gaaggcacgc tcgacagcgc ccgcaaaacg ctggagctgg aacgcggcct gttcaaatcg
     6841 ggcctggcca gcggatcgga tgtggccagc gccatcgcca cggtggaagg ggcggccagc
     6901 gatgtggcgg ccgatatcac caccctggcg caggacagga acgcgctcga cctgctcgtg
     6961 ggcgcccatg tggccgacga actgctgccc gacacgctgg acgggctgga tcatgcgatc
     7021 agcgcgccgc cggcgggcct gccatccgaa gtgctgcgcc agcggcccga tgtggtggag
     7081 gcagagcaca cgctgaaggg cacctatgcc accatcggcg cggcgcgggc ggccttcttc
     7141 ccggccatca ccctcacctc ctcggtgggc ctggccagca ccgcgctctc acagctgttc
     7201 acccatggcg cggcgcagtg gaatgtggcg ccatcggcca gcgtgccgct gctgggcggc
     7261 accaccggcg ccaacctcgc ctatgccaag gcccagcgcg actatgcgct ggccgcctat
     7321 gagctggcgg tgcagacggc gtttcgcgat gtggccaatg cgctggcccg gcgcggcacc
     7381 atcgccgacc agcgcgccgc gcaggacaag cagatgctgg cctatcgccg cgcctgggaa
     7441 ctggccgatg gccagcgccg ggcgggcacg ggcacctatc tggcagcgct agtggcccag
     7501 cgcagctatt attccgccca gcagacgcgc atcaccgccc agctggccga tctcagcaac
     7561 cgcttcacgc tttatgcagc ggtgggggca gatcccagcc tgtaaggcct agaggggccg
     7621 atcgcgcccc aacagcgctt ccacccgggc ggcggcggcc agcagcgccc ggtcggcaaa
     7681 gcgcggcgcc accaggctga ggcccagcgg caggccccct tgcgcgcgat aaagcggcag
     7741 gtggacaacc ggcaggtgca gctgtgtcca catctggttg aagatggggt tgcccggccc
     7801 gcgccccagt ggcgccacgc ccggcgcgct gggggcaagg aaggcatcga aaccggccat
     7861 cgccgcttcc actgcgatgc gggcggtgac ggtaaggtcg taggcggcgc gcaaggcctc
     7921 gtcgggatag cgggcgcggg cggccacccg ctggtggaaa tcggcatgca gcagggccgg
     7981 ttccgcccgg gccaggttga ggaaggcggc cgccccctcg cggaacagga tcgccatgaa
     8041 ggcctcctcc agcccgtcgc aggcggcagg cagcgccacc tcttccacca ccgccccggc
     8101 agcggcgagg cgggcggcgg catcggccat ggcggcttgg gtttccgcct cggcgcgggg
     8161 ccagtagggc gtgcggcaca ggccgatgcg caagctggcg cggggcggcg gcggcggcgc
     8221 gaaatcatag gtttcggcca gcagatcgag gccggcaagg ctgctggcat aaaggccgat
     8281 cgtatcgagg ctggcggcat aaagcttggc cccctcgcgc gagatctggc cccatgtggg
     8341 cttcagcgcg tggatgccgc agaagctggc cgggcggatg gtggagccgc cggtctgcgt
     8401 ggcgagcgcc agcggcacat ggcccgccgc caccgccgcc gccgagccag aggacgagcc
     8461 gcccggcgtg cgggcgccat catgcgggtt ggcagtggcg gcattgcggc cggcggcggc
     8521 aaattctgtg gtatccgtct tgcccaggat cagtgcgccg gctgcccgca aggtggccac
     8581 gcaaggcgca tcggcgatgg cgggcgcggc cagccccagc ggggaattgt ggctggtggg
     8641 catggccgcc acgtcgatca cgtccttgat gccgatgggg atgccggcca gcgggccggt
     8701 gggcccctcg ccccgatcct gcgcggcggc ggcagcgcga gcggcggccg gggccagatg
     8761 cgtccaggcg cggatctcgc ggtcggttgc ggcaatgcgg cgaagggccg cgtccagcgc
     8821 ggcggtggca ggcgtgctca tgggctgcat gctatcacgg cgggccttca tgtaaacatt
     8881 ttcattggcg gttgcacggg ggcttaaagg cctctatcgc attgcagcat gtcctcccct
     8941 tcccatagcc tgaagcaggc gctgatcagc accacgctgg tgcaggtgct ggccactgcc
     9001 atcgcgctgg cgctcacgcc cattgccccg cgcgcggcgc atgatttcgg catcgatccg
     9061 cattacatcg gctttcagat cagcctcatc tattgcgccg gcgcctttgc ctcggccgct
     9121 tcgggcacgc tgatccaccg gcacggggcg gtggcgatgg aagtgctgtc gctggcgctg
     9181 tttggcggcg ggctgctgct gctggccacg gcgcggctgc cgctgggcat gctgggctcg
     9241 gtgctgatcg gcgtgggcta tggcttgcag aacccggcct cgtcgcaatt gctcaaccag
     9301 gcctgcccgc cggaaaagcg cagcgtggtg ttctcgatca agcaggcggg ggtgccgctg
     9361 ggggcgctgc tggccagcct ggcgctgccc tggctggatg ggctgatcgg ctggcggctg
     9421 gcgctggtga tcggcgccat cggccctgtg gcgctcatcc tccacatcct gcgcgcgcac
     9481 cggcatgaag cgcaccggcg gcggccgggc ggcgctttcc tgcccggcct gatcgaggag
     9541 cagcggctgg tgtcgcgcga gccgcgcctg cgcgtgctgg cgctgctggg gctgctctat
     9601 tcggcggtgc agctttcgct ttcggcctat atcgtgctga tgctggtgga taatcgcggc
     9661 tggccgctgg tcagcgcggc ggcgatgggc ggcctgttgc agggctttgg cgcgctgggc
     9721 cgggtgttct ggggctggct ggctgatcgc acgcgggccg gccttgccat tctggcgctg
     9781 atcggccttg gcagcagcgc ggcgctggtg gcgctgccgt tgctgggggc gctgccggcc
     9841 gccctgcaag gggtgctgct gtgcgcgctg ggctttttcc tgtcgggctg gaatggcgtg
     9901 gcgatggccg aaatcgcccg cagcgcgccg cttgcgcaaa ccggccgggt gatgggcggg
     9961 gcgctggtct acacctttct gggcgtgatg ctggggccgg ccagctttgc cttcctctat
    10021 gaagcgctgg gccgctacga cagcaccttt gccgccgtgg cgctggccac cacgctgggc
    10081 gcgctggccg cggcctggcg ccatctggcc gagcgccacg cctgaaaaaa acaccgggcc
    10141 tgaaagaaca cctgcccccg cgcaaggcga gggcaggtgt cagttcccgt tcgggcgggg
    10201 tggccgggaa aggggttatg cgggctcggc aatatgcgcg cggcactgca tcagcggctg
    10261 caccttggtg gcaaattcat cgaggccggt gaggaattcg tcgaaagtca gcatgatgcc
    10321 cttggtgccg gggatgctgg cggcctcatc cagcaggcgg gcaacggtcg catggctgcc
    10381 gatgaaggtg ccgatgttga agttcaccgc gccctcgggc aggttgatgt ggcgcgaggt
    10441 ggaattggca tcgccggtcg tgtccttgct gccctggtcg gccatccagg ccagcgcgcc
    10501 catatcggca ccggccttgt aatgctccca cttggcgaag gcttcctcgt cggtctcgcc
    10561 catgatcacc atgcacagga tataggcccc cacatcgcgg cccgaacgct gcgaggcctc
    10621 caccagcttt tccaccgtgg gctggtgctg gaagggcttg ttgatgccgc cgcaggtgac
    10681 gaaattatag tcgccatagg tggcgacaaa atccatcccc cgttccgact ggcctgcgcc
    10741 aacgatctcg atcttgccgg aaggctgcgg ccccagcttg caatcgtcca tctggaaata
    10801 ctggcccttg aagtcggacg agccctgttc ccacagctcc tgcatcacgc ggacatattc
    10861 ctcggaatag tcgtagcgat agccgaaata atcatcgccc ggccacaggt tcatctgggc
    10921 atattcgccc ttggcccagc ccgagacgat gttgaggcca aagcggcccg gcgcgatcga
    10981 atcgatggtg atcgccatgc gcgcggcaat cgccggcggc agggtgagca cggcggtggt
    11041 ggcaaacagc ttgatcttgg tagtgacagc cgccagcccc gccatcagcg tgaagctttc
    11101 cagattataa tcccagaagc gcgtctcgcc gccaaagccg cgcagcttga tcatcgagag
    11161 cgcgaaatcg aagccatagc cctccgcctt ctggacgatc gccttgttca gttcgaagct
    11221 gggcatgtat tgcggggaat tcttggaaat cagccagcca ttgttgccga tcggcacgaa
    11281 aacgccaaga tccatggaag tctcctgtaa aatcaggtgg aagggagagg aggaagcggg
    11341 cgggccagcc agtcggtggc ccgttcaaag gcatgggcgg cgcccagcag caggctttcg
    11401 gcgcgggggg cgccgatcag ttgcaggccc agcggcaggc cggcaccatc aaagcccatc
    11461 ggcgtggaca gcgctggcag gcccagatag gaaatcggcc gggtgagcgc ggacagatcg
    11521 cccaccacct tgtccatcgc cgccccggca ttggcgccgg cggcctcgcc accgggcgtg
    11581 ggcatgcgca gggtgggcgt ggccaggata tcgcagcggg ccagcggccc tgccagcatc
    11641 gccgccagat cggcgctgcg cagccgccgc gcgcgcacat aggtggccgc cggcatggcc
    11701 agcccatgca gcaggcggtt gcgcacctgc tcgccataat cctgcgggcg gtcgcgcagc
    11761 cagtcgaggt ggagggcggc cgcttccggc gcccagacca gccccgccat cgccgccaga
    11821 tgcgcctgat cgggcatcgc cacctcgatc acccgcgcgc ccagcccctg cagcacccgc
    11881 accgcctcgg caaaggcggc ggccacttcg gctgccagcc cgtcggcata ataggccacc
    11941 ggcaggccga tggtgaggcc agccagatca ccccgcaacc cttgcgcgaa atcctcgcca
    12001 tcgccggcga tcaccgtcat cagccgggcc acgctgcggg ccgagcgggc cagcggccct
    12061 gccgtgtcca tgctttcgga aaggcccatc atgccctgct ggcccagccg gccatgagtg
    12121 ggcttcaacc ccaccacgcc gcagatcgcc gccggcagcc gcaccgagcc gcccgtgtca
    12181 gagcccagcg cgccggcgac gatccgcgcc cccaccgccg cgcccgagcc cgaggacgag
    12241 ccgccggtga tcgccgcgcc cgcccagggg ttgcggcagc ggccgaggtg ggcattgtgg
    12301 ccggtggggc ccatggcgaa ttcggccatg tgcagccggc cgatgtcgat ggcgccggcc
    12361 gcatccagcc gcgccagcag cgggcaggtt tcgcctgcca caaagccggc gcggattttg
    12421 gaaccgcagc ccgacacgcg gccctggcga tagaacatgt ccttgtgggc cagcggcacg
    12481 ccggccagcg ggcccagcac ctgcccccgg gcgcgggcca gatccaccgc gcgggccgct
    12541 tccagcgccg gcgcgccggc gatcgccacg aaggcgttga gatcggcgcg ggcatgggcg
    12601 gcggcaaggg cggcttcggt cagcgcctcg gcggtgaagg cgccttcagc gatggcctcg
    12661 gcctgcgcca ccagatcgag gctggcgagg gcttgcgcgg cgctcatgat tggcgcgcct
    12721 tgccggccag cagggcctcg gcagaggcgg cggggcaatc aaagaaggcc tctgccccca
    12781 gcgccgccac gccgcgcgcc agcaccgcgc catcggcctg ccaatgggcc aggcagcgcg
    12841 cttcccattc cggcgtgatg gcgctgtcgg ccaggcccag cgctgcggcc agccactgtc
    12901 gggccgcgct catggcaggc gcacgcccac ggcggcgcct gcctgcaggc acaggtatag
    12961 cttgggcaaa gcccgcataa atccctcatg ttttgctgtg gcgccccttg ccgggcgcgg
    13021 gcatcggcac gtctggaaac gtttacaaag atgccttgcg cggggccact gtcaagcccc
    13081 tatttgctgc gcatgcgaat gcgggggtaa acgccccccc gctctcagcc cccggctctc
    13141 agccctcggt ggggcgggcg gtgctttcgc gcaccaccag ctggtgcggc aggatcacct
    13201 cgcggcaggc gccatcggca tcgaggccaa aatccagcat cagccgcatc gcctgctcgc
    13261 cgatcagcgt ggttggcagc gccaccgtcg tcagcgccgg attgctgaat tcggagaagc
    13321 ggaaatcatc gatgccgatc acgctgatat cctgcggcac gcgcaggccc cgggcggcga
    13381 tggcatggat ggcgcccagc gccatctcgt cattctcgca caaaagcgcg gtgaacggct
    13441 cgccccggtc gagcaggcag cgcgcccctt ccacgcccga cagcagcgaa taatcgccgc
    13501 ctgccaccat tggctcgggc agatccaggc cgaaacggcg cagccccagg cgaaagccgc
    13561 gctgccggtc gcggctcatg cgctggttgg ccgggccgga aatgcaggcg atgcgcgtgt
    13621 ggcccagctg ggcgatatgc atggcggcat cggccgccgc ttccagatcg ttcacccgca
    13681 cgcgcggctg gttgctgcca tcctgatatt cgcaggccag cacgatgggc agcggggctg
    13741 cgtcagcggg ccgatcacgc agcacgccgg gcagctgcag gtcgagcagg atcatgccat
    13801 cggcctgctt gccggccacc atctcgtaat ggctgtcgag caggtcctcg cgcccctcgg
    13861 tgatcgccag cagcaccttg gtgccgctgt cgcgcgccac attttccacc ccctgcacga
    13921 aagcggcggt gaacgggttg gcgatatccg gcacggcgat gatcaccgca tcagccttct
    13981 gccggcgcag cgaggcggcc agcatgttgg gcttgtaatc gagatcacgg atggccgcct
    14041 cgatgcgctg ggcggtcttt tccttcacca gattgggctg gcgcagcgcg cgcgagacgg
    14101 tggcgatgga aaccccggcc ttggcagcga tatccttgat attggccatg gcttttccct
    14161 ccccagcagc ggctcttctg tggccgatcc ggcggctgcc agcaagaggg cagcccccgg
    14221 cccaggcgca gcgcaccaaa gttctatgcg cttaggccaa gggcggtgct acggcaagcg
    14281 cgatgccctt cgttttcacc cgcttctccg ccgggcggcg gaaacaggcc gatcaagccc
    14341 caccgcatgg gcctggccgc gcccggccca gtgaggattg ccagcaacac atgggccccc
    14401 acgcctccat gctccaaccc cgcaatggcc cattatcata atgatatata tagataatat
    14461 cattttttat cagaccacat cgcttcccgg ttgcaattgc ccgcaaagcc catattctga
    14521 tgaaaaggtt tacacataac aatgaggggt ctcaacgatg ttgcataaat ggagcctggc
    14581 cgccggtgtg gcgctggcca gtatcatgcc cgcgcttgcc catgccgaaa cgcccgccgc
    14641 cagcgcccag gccagcagcg gccttggtga aatcgtggtg acggccgaaa agcgcgagag
    14701 cacggcctcg aaaacgccga tcgccatgac cgtggccagc ggcgccgatc tgatccgcgc
    14761 cggtgttgcc gatgtgaacg ggctgagcaa tatcgccccc acgctgaaca tcgcgcagaa
    14821 caaccagaac acgatgatca ccatccgcgg catttccagc cgcgactaca ccgaaaccgg
    14881 caatccggcg gtggcggtca gcatcgacaa cttcttcctg cagaacggca ccgcgctcaa
    14941 cgtcggcttc tacgatctcg accgggtgga agtgctgcgc ggcccccagg gcacgcttta
    15001 tggccgcaat gcctcggccg gcgccatcaa catccagacc gccaagccca ccaagaagct
    15061 cgagggctcg gtctcgctgg gctatggcta caagaacgcg ctgctggtgg agggcatgat
    15121 caacctgccc gtgaccgaca atctgcaggt gcgcgcggcc ttctcggtgc ataaccgcga
    15181 tggctatcgc aacaacggtt cgctcagcac cggcaatgaa aacgtatcca agggcggcaa
    15241 tgacgatgtc agccaatccg cccgcctgca tgtgatgtgg gagccgacca gcaatttcac
    15301 cgcccttgtc acgggcgaat acacccatgt tggcggcgtg ggcccggtca tcaagggcat
    15361 tctggccgat caggtgaacg gcgatggcac gctgaagatc ggcagcacca ccaactggca
    15421 gctgtcgaac cagtcctata tcgacatcac ccagaagacc atccgcggca cgctgaccta
    15481 cgacctgggc tttgccaagc tgacctatat cggcggctat cgccagcagc acatcctgca
    15541 tggcaacgac caggatggcg gcaccaccta cagctatgcc ttccccaccg acggcaagac
    15601 cgacaccgaa aaccacgaat tgcgcatcgc ctccaacggc aacacgcgct ttggctggca
    15661 gggcggcctg tactacttcc aggatggcgg ccaggcgctt acctatttcc aggtgctggg
    15721 caatccggtg tataactact acaccttcaa ctacctcacc aatgcgcagt ccttcgctgc
    15781 ctatggccaa gtctactaca agatcttcga caatctgaaa ctgtcgggcg gcgcgcgcta
    15841 taccgaggaa tacaagaacc agctgggcta cagcatcatc gccggcacct acaccgatgt
    15901 gaactggaag tatcacggcg gcaaggacac ctaccatgtc ggcctcgact ggcaggtgga
    15961 tccgcatcac ctcgtctatg ccaagttcga caccggcttc aagaccggcg gcgtgcagaa
    16021 cctgcgctcc tatgggccgg aatcgatcaa ggcctatgaa gtgggcgcca agaaccgcct
    16081 gttcggcaac acggtggaac tgaacatcga cgccttctac tacgactatt cccagctgca
    16141 ggtgcagcag aacgaccccg aaaccgccat ttcctatatc tacaacgccg gcaaggcgcg
    16201 cgattacggc gtggaattcg aaggccgctg ggcgatcact cccaacgacc ggctggacgc
    16261 caacatcaac tacgtgaacg cccgttacac cgatttcgtc aacaccggcg tgcaatatgc
    16321 cgggcacgcc ctgccgcaat cgcccaactg ggcgctgggt ggcggcttct cgcatgattt
    16381 cgaactgaag accggcgcca aggtgaccgc gcgcgtgcag acgcgctatg aaagcaaatc
    16441 ctatttcggc tttgaaaacg ccaattacga gctgcagaag gcctatacca agaccgacct
    16501 gatcctcagc tacacgccgc tgggcaaggg cttctcggcc tcggccttca tctacaacct
    16561 cgagaattcc accattctca ccgattcgga agcggcgggc tatgccggcg gctatctggt
    16621 gcaattcgcc gatccgcgca cctatggcgt gcggctgaac tacagcttct gacggtcccc
    16681 ttttcccgca gaagctgcaa aaggcagggc ccggtcagcg atgaccgggc cctgttgcct
    16741 tggggaaggc tttgaaaagc cgggggcccg ccaaagcaag ccccccgccg gccggattat
    16801 tccgccgctt cggcttcctt ggcggcattg gccgcgtcga tctcggccgc cttggcttcc
    16861 accagcctga cgatgtgatc gagcatgtcc tcgctctgca catggtggtc ggtcacgccc
    16921 gagagataga ccatgtgctt gccattgccg ccgccggtga tgccgatgtc ggtctcgcgc
    16981 gcctcgcccg gcccgttcac cacgcagccc agcaccgaca gcgagatcgg cgtcttgatg
    17041 tgctgcagcc ggtcttccag cgccgacacc gtcttcacca cgtcaaagcc ctgccgcgcg
    17101 caggaggggc agctcaccac gcgcacgccg cgggtgcgca ggcccaggct cttcaggatc
    17161 tcgaagccca cccgcacttc ctcttccggc ggcgcggaaa gcgagacgcg cagcgtatcg
    17221 ccaatgccgg cccacaggag gttgccaatg ccgatggcgc tcttcaccgt gccgccgata
    17281 aggccgccag cctcggtgat gccgaggtgc agcgggcaat ccaccgcttc cgccaggccc
    17341 atataggccg ccacggcgag gaacacgtca gacgccttca ccgccacctt gtagtcgtgg
    17401 aagtcatggt cctgcagcag cttgatatgg tcgagcgcgc tttccaccag cgcctcgggg
    17461 caaggctcgc catatttttc gagcaggtcc ttctcgaggc tgccggcatt cacgccgatg
    17521 cggatggcac agccattagc cttggccgcg cgcaccactt cggccacgcg atcggcgctg
    17581 ccgatgttgc cggggttgat gcgcaggcac gccgcgcccg catcggccgc ttccagcgcg
    17641 cgcttgtagt ggaagtggat atcggccacc agcggcacct gggctgcgcg ggcgatctcg
    17701 cggaaggcgg tggtgctttc cacatccggg cacgacacgc ggatgatatc ggcgccggct
    17761 tcctcgcagc ggcggatctg gtcgatcgtc gccctggcat cgctggtcag cgtgttggtc
    17821 atggtctgca cgctgatcgg cgcatcgccg cccacggcca ccttgccgac catgatctgg
    17881 cggctcttgc ggcgggcgat gtcccgccag gggcgcaggc cggggttatg gtcagacatg
    17941 gcttactccg tagcaatcgg gaaggatgac atcagaaaca gggctggggc gccaagcgac
    18001 cgaccgggcc gaaagcggca agggatgcgg atcgaggggc agatcgggcg ccatcgagcg
    18061 cgcctatagc gcatccgcgc ccccccgcaa atgcgccatc gcgctggcgc ggtttttcat
    18121 ggtgcttgcg cggttttttg ccctgctggc gggggtttgc cggctcagcg gggcggggcg
    18181 tgcttgctgg cgcggcggtg gcccagctgc atgcccgctt ccagccggcc gcgcagctgg
    18241 gtgtaatagg ccagcaggaa ggcgtggatt tcctcggcat cggcgggcgg gtgccagccg
    18301 attctcgccg cgatcgcccc ggccacggcc gccatcggcg ctggcgcccc ttcgcgcagc
    18361 accttttcca gcacgcccag ctcatattcg ccataagcgg ccagttccgc cgcgctgaag
    18421 cgatagggcc caccggccgg cccctgcggc acggaaagcg ccgccgccag ccgccggtgc
    18481 ggcgcttcca ctacccaggt gccggccacg atatcgccgg gccgcagccg atcgcggttg
    18541 aacaagggca ggccggcaaa cagcaccagc caggccaggc ccgccgccat gctccagccc
    18601 agcccatgcg cgcccccgct ggtgcccagc agcccgggca ccgccagcgg cgcgctcaat
    18661 tccacctcgc gcagcagatt gcgggccagc acccggtcgg ccgtcagccg cgatccatcg
    18721 cgcgtggcga tgcgcaggcc cagcatgcgc ttgcccggcg ttgccccgcg cggccccagc
    18781 tcgaagccca gaaaccaggc attgcgcagc acgaagctgg ccagcagcca cagcacgaac
    18841 agcgcctgca acgcgccgcc cgccgccttg ccgccgccca gcccccgggc aatgcccagc
    18901 agcgccagcg ccagcgcccc cagcaccagc ccctgcagcg ccagatccag cagcaacgcc
    18961 cccagccgcg tgccgcgccc ggcaagccgc aggcgcaagg tcacgccctc ggcggtttgc
    19021 aacaggcgct cggctgcgtc ggacggggtc atggggcctc ccctccccgg cccacaaggc
    19081 cgaaataggc cagccacagc accagcatca gcccgcccag cgccatgcgg gcagcggtcg
    19141 gctcgatcag ctggcgggca aagccttcca gcagcccggc cacgaacagc atcgccacca
    19201 cccccagcat caccagcgcc gcccgccgcc cggcggccga gaccgcctcc agcacgcccg
    19261 cccggccggg aaaggccacc gcccgcccca tatgcagccc cgccgctgcc gccagcacca
    19321 gcgcgaacag ctctgtcgtg ccatgcaccg ccagccagcc ggccgcctcg ggcagcaggc
    19381 cggcgccggc aaacagccac aggaaagccc ccagcagcgc cgcattctgc accagcagca
    19441 gcaggctggg cacgcccact acaaagccca gcgcaaattc cagcagggcg atctgggcat
    19501 tatggccaaa cagataggcc gccagcacgg acagcccgct ctcctgcgtg tggccaaaca
    19561 gggtggcgcg cagggccgcg gggctggcgg cggggttgcg cccttgggcc atatcgccca
    19621 ccagcgcgcc aaaccattgc ggatcggccg cgcacaacag ccagcccacc gccgtgccca
    19681 gcgccagcac gataaggcaa atggcgatat ccaccgccag cgcccgcacc gcccggggcc
    19741 agccgcccgc cagaaaaccc gccagccagc cgcccagccc gatattgggg ccatacagct
    19801 ggaaccaggc gcgctgcacc agcgcttcca gataggcctg cacggcggca tccagcatgg
    19861 tgccgcgcgc caccgccagc gccgaggccg cctgccgata aagccccggc agcgccagca
    19921 gatcggcatc ggcaatggcg cgggggcggc ctttttccag caggccgacg atgcgctcca
    19981 gccgggccca gtcggcctcg cgggcgaggc gaaagcggtc ggagcccagg cttgcctgcc
    20041 agccttccgg cacggccggc gcgccagcgc gccagcgcgc ggccagatcg cgccagaacg
    20101 ccttcatgcc acccctccgc gcgcgcgcag gcccagatag gtatcgatca gccgcatatc
    20161 gagctggccg gccggcgctt ccagcacctc caccccggcc tggcgcagcc gggccagcac
    20221 gcgggcgcgt tcatccagca agccttgcgc gcccaccgcc cgggccagcg cgcccagatc
    20281 caccggcgcc ccccgcgcca tctcttccag ccctgcatcc tccagcgtga cgaacagcac
    20341 ccggtggcgc gccagcagcc ggccaatgct ttccaccatc atcgtcgcgc tggtgggatc
    20401 ggtgaaatcg gaaaacacca ccaccagcga gcggcggcgc agatgcgcca ccaggctggc
    20461 aagggcgagg gtgaaattgg gctcctcggc gcgataggcc agctctgcgg cggccgcgcg
    20521 caggcgggca aagccgccct ggccataaag gggcgtggcc aactccactt gcgcggcaaa
    20581 gccgaacagg cccaccctgt cgccctcgcg cagggccacc cagccggcgg caagggctgc
    20641 ggcaatcgcc cggtccagcc gcgtcaacct gcccagatcc tcgcacatcg ccccgccgca
    20701 atcgaaagcg aagacgatct ggttgttgcg ctctgcctca tattcgcggg ccatcaaccg
    20761 gccgtggcgg gccgacactt tccagtcgat ccgccggcga tccatgcccg gctggaattc
    20821 ggccatcgtc tcgaactggc tgccctcgcc gcgcaggcgc cgggcgatct cgcccgcccg
    20881 cgcatccttg agataggtcg ccagcgcggg cgagcgcacc acggccaagc tgggcagaat
    20941 gcgcaaggcc cgcgccaccg gccagcggcg ctggcaggcg ccaaggccca gcgggccggt
    21001 gcggcgcagg gccaggcttt ccacctcggc cagcccgcgc cgggccgggt ggcacagggc
    21061 ctgcccgcgc gcccggccgc tgccatcgcg ccgcagcgcc aggcgcagca cgccgcccgg
    21121 cgccagcagc gggccaaccg ccagcgccgc cgcgatgccg cccgccgggc cgccgccggc
    21181 cacgtccagc gcgatgggca gggcgccgcc cacttcggcc tcgccggcca tgtcgatccg
    21241 ccaatccgcg ctggcgccgg cggccagcgc agcatcggcc agcaccagca gccccatcgc
    21301 cagccccagc gccggcgcca gctgccacag atccgcgcgc accgccgcca gcagcagcgc
    21361 cgccggcgcc agcagcgcca gcagcagcac cgcgcgggcg gtgggcagca gcgccggccg
    21421 gccgggccgg ctggggcggc tggggaaaag ggcgcgcagg gccatcagcg ggcgctcagc
    21481 gcggggcctc ggtggcctcg atgaagccgg ccaccagcgc ttctgccgcc ttgccctcga
    21541 tctcggcggc cgggctcagc gtcaggcggt ggcgcagcac ggcaggggcc agcgccttca
    21601 catcatcggg cagcacataa tcgcggccat cgagcgccgc gcgcgcccgc gcggcctgcg
    21661 ccatcagcac gcccgcgcgc ggcgaggcgc ccacggcgat atcggcgctc tgccgggtgg
    21721 cgcggatcag ccgggcgatg taatcggcca ccggcgctgc cagctgcacg ccggccagcg
    21781 cgcgcgcggc cgcggccagc cgggcgccat cggccactgg cacgatgcca tgatcggccg
    21841 ggctttccgc cccggtgccg gcgccaaacc ggctgacgat gcgcacttcc tcctccagcg
    21901 ccggataggg cagcgccagc ttgaacagga aacgatccag ctgggcctcg ggcagcggat
    21961 aggtgccctg actttccacc gggttttgcg tggccaccac cagaaagcgg tctggcaggg
    22021 catggcgctg gccatccagc gtcacgccgc gttcctgcat ggcttccagc agggcggcct
    22081 gggtcttggg gggggtgcgg ttgatctcgt cggccagcag cagttcgcag aagatcggcc
    22141 cgcgggtgag ggtgaactgg ctggtctgga agttgaacag gttggcgccc agaatatcgc
    22201 ccggcatcag atcaggggtg aactggatgc ggccgaaatc cagcccgctg gcggcggcaa
    22261 agcagcgggc aaggaaggtc ttggcggtgc ccggcgggcc ttccagcagc acatggccgc
    22321 gcgccagaag ggcgatcagc agatggtcca ccacatcggc ctggcccacc acggccctgg
    22381 cggtttcggc gcggatggcg ccggccagct ctgtcagggc ggccaggggc aggtgttcgg
    22441 catcgctcat cgcgtcagcg tcctttcgat ggtgtggagc tgctgcgcgg cctgcagcag
    22501 ggcgccctcg ccatggggcg cggccagcgc ctccagcgtg gcgggaagat cgaggtcggg
    22561 ccggccgcgg gccgccagca ggcgggccaa agcggcctgt tgcgccggcg gcggcaggct
    22621 gcgcgccagc cccagcgccc gcgccagccg cgccgcagcg ccatcggcat agggcgcgcc
    22681 cagcacccgc caacgccgcg ccctgaggat aagcgcggcc atgttggcca ccagcaggcg
    22741 gcggccattg gcaggcgctg ccagcgcccg ctcgggcggg gccagccgca cgaacccgcg
    22801 ccagcccacc cccaaccccg ccagcagcag gcacaaggtg gcggccagaa acggcggggt
    22861 gaaggccagc gccaccagat tggtcggccg gccaaggcca ttgagggcca gatcgaagcc
    22921 gatggcgcgc gctgccccct gccggccggc cagcgcccgg cgcaccagcg cctcggccag
    22981 ggcggcgttt tcggcatggg caaggccggc gttgttcaaa agatccggct cgaagacgag
    23041 gatcagcggc ggcgccctgg gcggcaccca gccgggccga taggccgccg ccagcacccg
    23101 cccggcgcca tctgtcacca gcggggccag cgccgcgccg gtgccggcca gggccagatc
    23161 ggcgcggggc agcacggcgc cggtgccttc cggcccgatc accggcgggg ccgccggcgc
    23221 cacgccaatc gccacatcat cggcaaagcc tttccagccg ggcagggcca gcccggtcag
    23281 ccacacccag ccgggcgccg cgccgctggc cccctgggcg gcggccgtct gccatttggg
    23341 cagcaccacc agcaccggcc catgcggcag atgcgccatc acccgccgct ggatcgcccg
    23401 cccgtcagcc tcggccggcg gggtgagcac cagcaggccg ctgccttcgc ttgcgccaaa
    23461 gcccgcgctg ctgccgacag ccaccggata gcccgccacc tccagcaagg tggcaaggcc
    23521 ggcatagcca tcgagcccgc gcccgccggc atgggcgccg gctgccggca cctgcgcggg
    23581 cgcggcgccc gccagccagg ccagccccag cagcagggcg cccatcgccg ccaccaatgc
    23641 tagcgcggcc ttgccgctga aggggccctg cccgctcatc gagccgcgcc ctcggccagc
    23701 ggcacctgcg cgaaatcggc ataagcggcg cgcgcggcct gccagtctgc cagcgccagc
    23761 gggcgcaggg cataacggca ctgctccacc cgcgcggcga tgaggccaaa agccatgcgc
    23821 gccgctgccg gcagccgcgg ctggccggcc agttcgcgcg cggtggtggc cgggtgcagc
    23881 aggccggggc gggcagcggc gatctgcgcc acgctgcgcg ccagcagcag atgcaccgcc
    23941 tcgtcgaacg cgccggccgc cgccaggctt tcggcatcgg ccagcagagc ggcggcttcg
    24001 gcggcatcag gtgcccaggg cgcggccttg tcggccaggg cgtggcggcg gcgcgccggc
    24061 cagcgccgca ccagccacca gccagctgcc agcaccagcc ctgcccccag caccaccgcc
    24121 aggctggcca tccacggcca ggccccgccc agcgcatggc cggccgggcc gaacaggcgg
    24181 gcaaacagcc gcgccagcca ctccagcgcc ttcaccagcc attccagcca gaacggcggc
    24241 ttgtagggcg caggcggcgg cagcggggca aactggatct cgccatgggc cctcagcgcc
    24301 cgccaatggg ccagcgccgc cggatcgggc cgggccatgg ccgccgatgt ggcagcggat
    24361 gtggcaatgg catcacgcaa gcggcaggcc cccttggacg cctaggtggc atggcgccca
    24421 agcacttgca aggggtggca aaaaccaccc gccttgcccc cgcggccgtt cagccctgcc
    24481 tgtaaccagg caatcagccg cgcgggcggg cctgcggata gctgcccaga aggcgcaggt
    24541 tcttgcagtg gaaagcggct tcctcgatcg cccggtccac cgccggatcg cccggccggc
    24601 caacgatatc ggcatagaag gtggtggcgg caaagctggc ccccttctga tagctttcca
    24661 gcttggtcat gttcacgcca ttggtggcaa aaccgcccag cgccttgtag agcgcggcgg
    24721 gaatattgcg cacctcgaag atcagcgtgg tcatcgtcac ctgcccggcc agttccgccg
    24781 gatcgcgggg cgcgcgggcc agcaccacga agcgggtggt gttgtcggcc gcatcttcca
    24841 cattatgctc gacgatatcg aggccataga gctgcgctgc cagcgccggg gcaatggcag
    24901 cggcggccgg atcgccctgc tcggcgatga aggcggcagc gccggccgta tcggcatagg
    24961 ccagcggcgt gatgccgcgc gcgcgcagat aatggcgcga ctggcccagc gcctgcgggt
    25021 gcgaataggc cgcggcaaac ggcccgcgcg cgcccggcag cgccatcagc gcatgttcga
    25081 tcgccaggaa atgctcgccc acgatggcaa ggccgctttc gggcagcagg aaatggatat
    25141 cggccacgcg cccgtgctgc gaattctcaa tcgggatcat cgcccggtcg gcgcggccct
    25201 ctttcaccgc atccagcgcg tcttcaaagc tgaagcaggg cagcggcagg caggccggat
    25261 catattccag cgcggcgcgg tgtgaattgc agcccggcgc gccctgaaag gcgaccgcgc
    25321 gggcaggatc ggtttcggca agatcggtca atgttttgac ctggatcaga gcgggttcgg
    25381 caaagctgtg catagcaatt ccggcgctta ggccggtgca ggcgatcccg caatggctct
    25441 tgcgcttggt ttggtggctc tctacaccga ggcaaaattt tttcgcaggt ttttggggcg
    25501 gggctttatc gatgacaggt cgtttcaata ccatcgccgg ttgggttctg ggttcgggca
    25561 ttgtggcgct gggccttgcc agcctctcgg cccattattt ctcggccgac aagcccgagc
    25621 gccccgagac catgggctat gccatcgagg gcgtggcggg cggggccaag ggcccggccg
    25681 aagtgcctct ggaagtgctg ctggccaagg cagacccggc caagggccag gaagtgttcc
    25741 agaagtgcgc ctcgtgccac accatcaccc agggcggcgc caatggcatc gggcccaacc
    25801 tctatggcat tctgggcgat gccattgccc aggggcgcgg cggcttcgcc ttctccgacg
    25861 ggctgaagaa gcatagcggc aagtgggact tcaaggagct gaacacctgg ctcaccaacc
    25921 cgcgcgccta tgccgatggc accaagatga ccttcgccgg cctggatgac gcggccgacc
    25981 gcgccaatgt gctggcctat ctgaacaagc agggctccaa cctgccgctg ccggcgccca
    26041 aggcggatgc caagcccgct gcaggcgatg ccaagccggc ggctggcgat gcggccccgg
    26101 ccgcctccag cgcagcaaag taaaaaggcg ggcggcgagg gggttcttct cccccctcgc
    26161 ccgcttttgc gcgcgggctg ctcagtccgc cggcgccgcg cggcccttga agccctgcgc
    26221 gatcacatac cactccgagg aatccttgcg gctggccggc ggcttggcgt gtttcaccgt
    26281 ggtgaaatgc tgcttcagaa tcgccagcag gcccggatcc gtgccgccgg caaacacctt
    26341 ggccacaaag gccccgccct tgcccaggtt ctggatggca aaatccgccg ccgtttccac
    26401 cagccccatc gtgcgcagat ggtcggtctg cttgtggccc acggtgttgg cggccatgtc
    26461 ggaaagcacg agatcgggca ggccgcccag cgcgtccatc agcgcatcgg gggcttcatc
    26521 ggccatgaaa tccatctgga acaggatcgc cccgtcgatc gggtcggtgg gcagcagatc
    26581 tatgcccaca acgcgggcct gcggggccct ggcgcgcacc acctgcgtcc acccgcccgg
    26641 cgccacgccc agatcgacca cgcgcctggc gcccttcagc agggcatatt tgtcgtccag
    26701 ctcgatcagc ttgtaggccg cgcggctgcg atagccatcg gcccgggcct tcttcacata
    26761 gggatcgttc aactggcgct gcagccagcg ggccgaggaa gcggtgcgct tctttgccga
    26821 tttcagccgc tggccgaggt ctttcacgcc gcgcgtcatt gcttctctcc ctcgccggcg
    26881 gcgcctggcc ggccatccac attgatcagc ccgcgcaaga tgccctcgcg gatgccgcga
    26941 tccgccacgc ccagccgctc ggccggccac agatcgagaa tcgcatcgag aatggcgcag
    27001 cccgccacga ccagatcggc ccgctcgcgc ccgatgcagg gcaggccccg ccgctctgcc
    27061 accgacatgc cagacaatcg ctcgctgatc gcccgcatgg ccggcgccgg cacgatcgcc
    27121 ccatccaccg cgcgccggtc gtattgcggc agatcgaggt ggaggctggc gagggtcgtc
    27181 accgtgccgc tggtgcccag caggcgcagc ggccgctcat gcgcgcgcca gggcgccacc
    27241 cgcccggcaa aaccagccag gctttccgcc accatggcgc gcatgcgggt gtagcgatca
    27301 aggcgctgct cgcgcgtgtc ttcgggcccg gtgcaggttt cgtagagcga gacgacgccc
    27361 cagggcacgc tcacccaatc gcgga
//
LOCUS       DASRYE010000077         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_20449,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000077 DASRYE010000000
VERSION     DASRYE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20449"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFF98_04330"
     CDS             complement(<1..550)
                     /locus_tag="VFF98_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62887.1"
                     /translation="MRIAYFVHDVQDAAVVRRTGLLRDEGLEVTVAGFRRRALLAGEP
                     GRQGAVDLGLTRDGRLLRRVLSVLGHVLRPGRIAAVAAGADVLVARNLEMLVLARRVR
                     RPGQRLVYECLDIHRLLLGMGPASRLLHRIEDWALQGTDLLVISAPAFLNSYFRARRG
                     YRGAALLVENKVPAAEARLPAPA"
     gene            1042..2487
                     /locus_tag="VFF98_04335"
     CDS             1042..2487
                     /locus_tag="VFF98_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZU62888.1"
                     /translation="MAFGRAASGLSLLAAGLLGAAPAARAGDLIEALAKAYAGNPDIE
                     EARAQLRAVDETVQIARAPGLPSIATSVRYQYSVNPGLNNYTSPTRTFAADAQVSIPI
                     YQGGSVRNAIRAARTRVNGGRHDLEAVETNIFAEVVTAYMDVLRDESVVDLNQRNVAA
                     LSVNLKATEARFAHGELTRTDVAQSVERLNLARGVLHTAEVNLATSREKYVQLVGSPP
                     DQLTQPPPLANLPAGADDAVEAAMQTNPDLLSAQESAKAAGFDAKVAGSARLPKIAVV
                     GTYEYDNYFNTYGQLYAPYYYQFDKISQVTVQASFPLFQGGQPAAEQRKARATQSAAL
                     EHANSVERQVVQATHASFIAWRASDNIIEANHRAVEAAQQSLDGVRAENKIGDRTILD
                     VLNAEQELLNAQVQLVAARHDAYVAAFNLLAAMGRVTPRDLGFDESQIYDPAANYRRV
                     RGSIWDWGKGPKPVAVSSGTAAVTMQNGDVK"
     gene            2558..3145
                     /locus_tag="VFF98_04340"
     CDS             2558..3145
                     /locus_tag="VFF98_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU62889.1"
                     /translation="MRLMFKACGRGGLALALAGAAMASGACGVAQAGEPAQPAALAGY
                     VLGADDKVHINVFGEEKLTGDYTVTSEGIISFPLIGSVPAAGRSLGDVQEEIRRRLAD
                     GYLLDPRVAIEVGTFRSLYILGEVNKPGEYPYRTNVTLEQVVATAGGYTYRANRRQVL
                     LRHLGEEKDEKLKLGEAMALHLQPGDTVRVPERFF"
     gene            complement(3229..3819)
                     /locus_tag="VFF98_04345"
     CDS             complement(3229..3819)
                     /locus_tag="VFF98_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZU62890.1"
                     /translation="MVDIIMAMAMIVVFTPVMILISIAIFLTDPGPILFRQNRLGRDG
                     AYFPCLKFRTMVVDAERQLQRILDSNPEARRSWELDHKLKKDPRITPIGTFLRRSSLD
                     ELPQLFNVLLGDMSLVGPRPISDAERVRYGRYFQDYCSVRPGITGLWQISGRNDVSYR
                     RRVTLDVVFARNISLELYLRIAVMTVPAVLFARGSY"
     gene            complement(4309..4458)
                     /locus_tag="VFF98_04350"
     CDS             complement(4309..4458)
                     /locus_tag="VFF98_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62891.1"
                     /translation="MHAPARAAGEDGIDVMEARPYPLPLRALIWLGAAASSWGLLARA
                     LGWIG"
     gene            4946..5413
                     /locus_tag="VFF98_04355"
     CDS             4946..5413
                     /locus_tag="VFF98_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62892.1"
                     /translation="MATVNGVTVAADTWGVVVGTGNGDHISGGSGNDTLEGTGGNDIY
                     YGGAGNDTFIITASSLAASSGTTDNINALAVIFDFQGAGTWTSTNNDFLALTGFGAGS
                     TLTFDHYGGTQTGALDVTQQYYTVHDTTTGNNYTIYIHSLDGSQIKAGDYAFY"
BASE COUNT         1059 a         1782 c         1717 g          893 t
ORIGIN      
        1 gggcaggtgc tggcagccgc gcctctgccg ccggcacctt gttttccacc agcagcgccg
       61 ccccgcgata gccccgccgc gcgcggaaat agctgttgag gaaggccggc gcgctgatca
      121 ccagcagatc cgtgccttgc agcgcccaat cctcgatccg gtggagcagc cggctggccg
      181 gccccatgcc cagcagcagc cggtgaatat ccaggcattc atacaccagc cgctggcccg
      241 gccggcgcac ccggcgcgcc agcaccagca tttcgaggtt gcgcgccacc agcacatcgg
      301 cgccggccgc cactgccgcg atgcgccccg gccgcagcac atggcccagc accgagagca
      361 cccggcgcag cagccggcca tcccgcgtga ggcccagatc aaccgcgccc tgccgccccg
      421 gctcccccgc cagcagggcc ctgcggcgaa agcccgccac cgtcacctcc agcccctcgt
      481 cgcgcaggag gccggtgcgg cgcaccacgg cggcatcctg cacatcatgt acgaaatagg
      541 cgatccgcat gggcagctct ccgcgggaac gtgcgatggg cgggggtggc gagcgacgct
      601 gatgcggata cgccccctgc ccgatcgcgg gtttcaggaa tgccttgcgg gtgctgccca
      661 atttccgccg ccatcgcagc ccgatctgca tcggaacccg atggaacggc gggcattctg
      721 atccgaacct gcggcaaagc cgggtacggc cgaaaagccc gcccgcccgc ctgcccgccc
      781 acctgtccgt ttgcgccgaa atgaagccat cgcgctgcac tgcacaccgc gcatttgccc
      841 ggcttgccgg cgctggtgcg cccgctggcc ggtagcggcc gcgcaaaggc tccagaacgg
      901 aaggaaggcc tgtggaatcc ccgtaaggag acggcaagaa tcccccgcgc acccaagccc
      961 tgtctggcaa ggaaaaccgg gatcatgtcc tttcgccgcc gcaattgctc tcaccgccct
     1021 tgcatcgtgc ctgccggccc tatggctttt ggcagggcgg cttccggcct gtcgctgctg
     1081 gcggcagggc tgctgggggc cgcgcctgcc gcccgggccg gagacctgat cgaggcgctg
     1141 gccaaggcct atgccggcaa ccccgatatc gaggaagcgc gcgcgcagct gcgcgcggtg
     1201 gacgagaccg tgcagatcgc ccgggcgccg ggcctgccca gcatcgccac cagcgtgcgc
     1261 tatcaatatt cggtgaaccc gggcctcaac aactacacca gccccacccg cacctttgcc
     1321 gccgatgcgc aggtctccat ccccatctat cagggcggca gcgtgcgcaa cgccatccgc
     1381 gccgcgcgca cccgcgtgaa cggcgggcgc cacgatctgg aagcggtgga aaccaatatc
     1441 ttcgccgaag tggtgacggc ctatatggac gtgctgcgcg atgaatcggt ggtcgatctc
     1501 aaccagcgca acgtggccgc gctcagcgtg aacctgaagg cgaccgaggc ccgcttcgcc
     1561 catggcgagc tgacgcggac cgatgtggcg caatcggtgg agcggcttaa cctggcacgc
     1621 ggggtgctgc acacggccga agtgaacctg gcgaccagcc gcgagaaata tgtgcagctg
     1681 gtgggcagcc cgcccgacca gctgacccag ccgccgccgc tggccaacct gccggcaggc
     1741 gccgatgatg cggtggaagc cgcgatgcag accaaccccg acctgctatc ggcgcaggaa
     1801 agcgccaagg ccgcggggtt cgacgcgaaa gtggccggtt ctgcccggct gcccaagatc
     1861 gccgtggtcg gcacctatga gtatgacaat tacttcaata cctatgggca gctctacgcg
     1921 ccctattact atcagttcga caagatctcg caggtaacgg tgcaggccag ctttccgctg
     1981 tttcagggcg ggcaacccgc cgccgaacag cggaaagctc gcgccaccca atccgccgcg
     2041 ctcgaacatg cgaacagtgt ggagcggcag gtggtgcagg ccacccatgc ctccttcatt
     2101 gcctggcgcg catcggacaa catcatcgag gccaaccacc gcgccgtgga agccgcgcag
     2161 cagagcctgg atggcgtgcg ggccgaaaac aagatcggcg atcgcaccat cctcgatgtg
     2221 ctgaatgccg agcaggaact gctgaacgcg caggtgcagc tggtggccgc caggcatgac
     2281 gcctatgtcg ccgccttcaa cctgctggcc gcgatgggcc gggtaacgcc gcgcgatctc
     2341 ggtttcgacg aatcgcagat ttacgatccc gccgccaatt atcgccgcgt gcgcggcagc
     2401 atctgggatt ggggcaaggg ccccaagccg gttgccgtgt cgagcgggac ggccgctgtc
     2461 accatgcaga atggcgatgt gaaatagcgt tttatggccc ggcagatggg ggcgtgcgcc
     2521 ttgtgccgcc gggcgccaac agacaatgga ggtagctatg cgattgatgt tcaaggcgtg
     2581 cggccggggc gggctggctt tggctcttgc cggcgcggca atggcaagcg gcgcttgcgg
     2641 cgtggcgcag gcgggggagc cggcccagcc ggcagcgctg gccggctatg tgctgggcgc
     2701 cgatgacaag gtgcacatca atgtgttcgg cgaagagaag ttgaccggcg actatacggt
     2761 gacgagcgag ggcatcatct cctttccgct gatcggcagc gtgccggccg ccgggcgcag
     2821 cctgggcgat gtgcaggagg aaatccgccg gcgcctggcc gatggctatc tgctcgatcc
     2881 gcgcgtggcg atcgaggtgg gcacgttccg ctcgctctac attctggggg aagtgaacaa
     2941 gccgggcgaa tatccctatc gcaccaatgt cacgctggaa caggtggtgg caacggccgg
     3001 tggttacacc tatcgggcca atcgccggca agtgctgctg cgccatctgg gcgaggagaa
     3061 ggacgaaaag ctgaagctgg gcgaggccat ggcgctgcat ctgcagccgg gcgacacggt
     3121 gcgggtgccg gaacgcttct tctgaagctt ccccccctgc ccccacgcga aaagggccgg
     3181 agcgtttgcc ccggcccttt ttcgttgccc ccttgcggtg gtgcctggtc agtaggagcc
     3241 gcgcgcgaag agcaccgccg gcacggtcat cacggcgatg cgcagataaa gttccagcga
     3301 aatgttgcgg gcaaagacca catccagcgt cacccgccga cgatagctga catcgttgcg
     3361 cccgctgatc tgccacaggc cggtgatgcc gggccgcacc gagcaataat cctgaaaata
     3421 gcggccatag cgcacgcgct ccgcatcgct gatcgggcgc gggccgacaa ggctcatatc
     3481 ccccagcaag acgttgaaca gctgcggcaa ttcatccagg ctgctgcggc gcaaaaaagt
     3541 gccgatgggc gtgatgcgcg gatctttctt cagcttgtga tccagctccc aactgcggcg
     3601 cgcttccgga ttactgtcga ggatgcgctg caattgccgc tccgcatcga cgaccatggt
     3661 gcggaatttg aggcagggga aataggcgcc atcgcggccc agccggttct ggcgaaacag
     3721 gatcgggccc ggatcagtca ggaagatggc gatagatata agtatcatta cgggtgtaaa
     3781 aaccactatc atcgccatcg ccatgatgat atccaccatg cgtatgagat aatcatcatg
     3841 gacagccgca tttcctgagt gaacgggccg agatgaaacg gcaaatttca ggtcaacagc
     3901 gttttgcatg atgtgccctc tattgtcgtt gccggcaatt tgccgtcacc ttgtcgtatg
     3961 aatgacggaa ggtgattttg cagatgctaa gtaagctagc tgcaattagc ctctctgacg
     4021 agaagccggc gggcatagaa aaatcggaac ccgattggtt tgggccgccc atgggcctga
     4081 aacgggcggc catggcgcga tctgcggcgc ggtggcgggg gaataaaccg gctgcgggca
     4141 agcaaaggcc gcccatgctg ccaggggcgg cacccttccc tatttagttc aaaaacggat
     4201 acagataccg catagatacg tggtctgcgg gggcgacacc ccctccacag ggccgattat
     4261 gttgcaatgc agcacaaggg aatcgccgcg ctcaggccgc cagggccctc agccgatcca
     4321 gcccagcgcc ctggccagca agccccaact cgatgccgcc gcgcccagcc agatcagcgc
     4381 ccgcagcggc aggggatagg gccgcgcctc catcacgtcg atgccatcct ctccggccgc
     4441 ccgggccggc gcatgcatct gccccgcgca aagcgcgccc tggcaggctt gcggaagcgg
     4501 cgagcgggaa tgggaggaag catttgtcat ggacgtactc acacggggga aacgcttggc
     4561 aagcattact ctacaggact taataagcgt taataagccc attctgcatt cgcgcctaac
     4621 cacggcgggc gcctgccaac cggccgggcg tgcgggctgg ccccggccct gttggcgggc
     4681 cctgccggct tgcccctctt tggatcaaaa gcaatgactt gtattccatc acctattcaa
     4741 ttttcataag tagatcatct catcgagatc ggctttttat cgatccaaat cagattaatc
     4801 tatgtgcatc cgcaatattt attgaagacg aaatggttta tcgactttct ccgggcaagc
     4861 aaaacgcctg cccacaccgt aacggtctga tctaggtgtt ttatctgatg agactgttct
     4921 gtgcgacacg gcaaggagaa atagcatggc aactgtaaac ggggtgaccg ttgcagccga
     4981 cacctggggt gtcgtggttg gcaccggcaa cggggaccac atcagcggcg gcagtggcaa
     5041 tgacacgctg gaaggcaccg gcggcaacga catctattat ggcggcgccg gcaacgacac
     5101 cttcatcatc actgcgtcct cgctcgctgc atcctccggc acgaccgaca acatcaacgc
     5161 gctggcggtg atcttcgact tccagggcgc cggcacctgg acgagcacca acaacgactt
     5221 cctcgcgctg acgggcttcg gcgccggctc gacgctgacc ttcgaccatt acggcggcac
     5281 gcagaccggc gcgctggacg tgacccagca gtattacact gtgcacgaca ccacgaccgg
     5341 caacaactac acgatctaca tccactcgct ggatggcagc cagatcaagg cgggtgacta
     5401 cgccttctat tgagccgaac agaggcctgc gcccgcccca cgaaaggggc g
//
LOCUS       DASRYE010000078         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_251751,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000078 DASRYE010000000
VERSION     DASRYE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251751"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1162)
                     /locus_tag="VFF98_04360"
     CDS             complement(35..1162)
                     /locus_tag="VFF98_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU62893.1"
                     /translation="MNVTSREHTGAPGDIGADIGADVGLVSATSADGRPVLSFDTDAH
                     HQLGPRARALVFTDPRSRALLPLLERVAPSTANVLVAGETGTGKELVARYVHALSARA
                     DQPFVAVNCGAFAEALVEGELFGYERGAFTGAHIARPGWFEAANGGTLFLDEVGDLPL
                     SLQVKLLRVLQEREVVRIGARVPIRLDVRVIAATNVDLRRAVDEGRFRADLFYRLQVV
                     TVPLLPLRERRADILPLARHFLHAYGDRLQPGRLELTPSAEEALFRHGWPGNIRELEN
                     AIHRATLICQDGRIGVADLALGGEGLPRPADASGGPEEAVRALVVRLLQDGHADLLDR
                     IIATTVSETYHLCGANQIKTAEALGVTRNVIRTYLKNLGLI"
     gene            complement(1269..2309)
                     /locus_tag="VFF98_04365"
     CDS             complement(1269..2309)
                     /locus_tag="VFF98_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU62894.1"
                     /translation="MKQAGLPEAIWMMRCPVPAASGVAWALGWMDEAFGRDGIAVRRV
                     LEDGLYPVVEDPDVQARHFFREGGNIQPIAARVRGAPTRVIGLTWIDERQVILVRPGK
                     RVQEPADLRGLRFALPGFARSRGESIARGMALHGIISALGLGGLTLDDVRLVEVPAPP
                     TERASPQGMRRLWLGLEWLAAGRVDAVYVKGAAAADIAARLGLEVALELDAYPSRLAR
                     INHGTPRPILVHEHMIEHHFELVVRFLATSLRAADWAARNPAGLRPILAREMFSQEAG
                     VATAYRREGHLALHPDLSAERIDLLRSQAQFLWSHGFLDGQLDVDGWIDPAPLAEAMA
                     RRAAAHSPCPAL"
     gene            2623..>3081
                     /locus_tag="VFF98_04370"
     CDS             2623..>3081
                     /locus_tag="VFF98_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62895.1"
                     /translation="MHHISRIPPAVSALPAPADDSPAGRHAFSRRQVSGGALGLLALG
                     LAGGLAGCRRGGAGLDVVNLAAIAYPYKGGQVLNGLNGVVEEQGWLKGELGKRGIALN
                     FVPVPTAVGAPQINEGFSGRKIDFASYGDFPAIIADAGGVPLKLVAPAGQG"
BASE COUNT          471 a         1103 c         1053 g          454 t
ORIGIN      
        1 gccccgccat gccgggcgcg ggcgcggggg cggttcagat cagccccagg ttcttcagat
       61 aggtgcggat gacattgcgc gtcaccccca gcgcctcggc ggtcttgatc tggttggcgc
      121 cgcacagatg gtaggtctcg ctgaccgtgg tggcgatgat gcggtcgagc aggtccgcat
      181 ggccatcctg cagcaggcgc accactagcg cgcgcaccgc ctcttccggc ccgccgctgg
      241 catcggcggg gcgcggcagg ccctcgccgc ccagcgccag atcggccacg ccaatgcggc
      301 catcctggca gatcagcgtg gcgcggtgga tggcgttttc cagctcgcgg atattgcccg
      361 gccagccatg gcgaaacagc gcttcctcgg ccgagggggt aagctccagc cggccgggct
      421 gcagccggtc gccataggca tggaggaaat ggcgggccag cggcaggata tcggcccgcc
      481 tttcgcgcaa gggcagcagc ggcacggtga ccacctgcag gcgatagaac aggtctgccc
      541 ggaagcgccc ctcgtccacc gcccggcgca gatcgacatt ggtggccgcg atcacccgca
      601 catccagccg gatcggcacc cgcgcgccaa tgcgcaccac ctcgcgttcc tgcagcacgc
      661 gcagcagctt cacctgcagc gagagcggca gatcgcccac ctcgtcgagg aacagcgtgc
      721 cgccattggc tgcctcgaac cagccgggcc gggcgatgtg agcgccggtg aaggcgccgc
      781 gctcatagcc gaacagctcg ccttccacga gcgcctcggc aaaggccccg caattgaccg
      841 cgacaaaagg ctgatccgcc cgcgcgctga gcgcatggac atagcgcgcc accagttcct
      901 tgccggtgcc ggtttcgccc gccaccagca cattggcggt gctgggcgcc acccgttcaa
      961 gcagcggcag cagcgcgcgc gaacgcggat cggtgaagac cagcgcgcgg gcgcgcgggc
     1021 ccagctggtg gtgggcgtcg gtatcgaagc tcagcaccgg gcggccatcg gcgctggtgg
     1081 ccgataccaa gccgacatcg gcgccgatat cggccccaat atcgcccggc gccccggtat
     1141 gctcgcgcga ggtcacgttc atcggttctc ccctgtccac aggcaggata acagttttat
     1201 tcctagttca ttggtagaaa ttagtgtcgg gggcctagct gggcttttca cagggcgggg
     1261 caggcgcttc acagggcagg gcaggggctg tgggcggccg cgcgccgggc catggcctcg
     1321 gcaagcgggg ccgggtcgat ccagccatcc acatccagct gcccgtcgag aaagccgtgg
     1381 ctccacagga actgggcctg gctgcgcaac aggtcgatcc gctcggccga gagatccggg
     1441 tgcagggcca ggtgcccctc gcggcgatag gcggtggcaa cgccggcttc ctgcgagaac
     1501 atctcacgcg cgaggatcgg gcgcagcccc gccgggttgc gcgctgccca gtctgccgcg
     1561 cgcaggctgg tggcaagaaa ccgcacgacc agctcgaaat gatgctcgat catgtgttca
     1621 tgcaccagga tcgggcgcgg cgtgccgtgg ttgatgcgcg ccagccggct gggataggca
     1681 tccagctcca gcgccacttc cagccccagc cgggcggcga tgtcggcagc tgccgcgcct
     1741 ttcacgtaaa ccgcatcgac acgccccgcc gccagccatt ccagccccag ccacagccgg
     1801 cgcatgcctt gagggctggc cctttcggtg gggggcgcgg gcacttccac cagccgcaca
     1861 tcatcgagcg tgagcccgcc cagccccagc gcgctgatga tgccatgcag cgccatgccc
     1921 cgggcaatcg attcgccccg gctgcgggca aagccgggca gggcaaagcg caggccccgc
     1981 aggtctgccg gctcctgcac gcgcttgccc gggcgcacca ggatcacctg ccgctcgtcg
     2041 atccaggtga ggccgatgac gcgggtgggc gcgccgcgca cgcgcgcggc gatgggctgg
     2101 atattgcccc cttcgcggaa gaagtgccgg gcctgcacat cggggtcctc aaccaccgga
     2161 tagagcccgt cttccagcac ccggcgcacg gcaatgccat cgcggccaaa ggcctcgtcc
     2221 atccagccca gcgcccaggc cacgcctgat gccgccggca ccgggcaacg catcatccag
     2281 atcgcctcgg gcaggcctgc ttgcttcatc aacatccgct ctcccttgat ggccggggga
     2341 agaggccccg tgccatcgct gcatcacgcc gcccggcaag caagagccgg gccaccctgc
     2401 gccttcctca gccttcccgc gcgcgcgggg cccggcaggc gccagccggc gccccggctg
     2461 cgcataaaac cccagcgcgc tggtgatttt tgcgcagccc gccacccctc gcaccggcaa
     2521 aagcgcgcaa tcgcgcgccc cgcgcggttt ggcacagccg atgcagggca gtggcgcgct
     2581 gcggcgcatt cggtttcctt tcaggcaagg ctggcttttc ccgtgcacca catctcccgc
     2641 attccccctg ccgtttccgc cctgccggcc ccggctgacg acagccccgc aggccgccac
     2701 gccttttccc gccggcaggt gtcaggtggg gcgctggggc tgctggcgct ggggctggct
     2761 ggcgggctgg cggggtgccg gcggggcggg gcggggctcg atgtcgtcaa ccttgcggcc
     2821 atcgcctatc cctacaaggg cgggcaggtg ctcaatggcc tcaatggcgt ggtcgaggag
     2881 cagggctggc tgaagggcga gctgggcaag cgcggcattg ccctcaattt cgtgccggtg
     2941 cccaccgctg tcggcgcgcc gcagatcaac gagggctttt ccggcaggaa gatcgatttt
     3001 gcctcctatg gcgatttccc cgcgatcatt gccgatgccg gcggcgtgcc gctgaagctg
     3061 gtggcgccgg cggggcaggg g
//
LOCUS       DASRYE010000079         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_75608,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000079 DASRYE010000000
VERSION     DASRYE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75608"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFF98_04375"
     CDS             <1..524
                     /locus_tag="VFF98_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZU62896.1"
                     /translation="KAARLAFRAPPLRRSGPLSAAERAAAIALAGVAEEQVAEAFHGD
                     NGPGWQVLRLHSAAAVLAARPAPHAPANTDIGLLGPWPADAPEADGAAWELRGLFSLP
                     TGALGEDPVTGSLNAAVACHLFATGQAAGDYVAAQGRCVGAAGRIHVHRDAEGIWIGG
                     DVAMVARGGQLAG"
     gene            complement(535..1380)
                     /locus_tag="VFF98_04380"
     CDS             complement(535..1380)
                     /locus_tag="VFF98_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HZU62897.1"
                     /translation="MIASAALLPQAAGFHVGHPDVVMLGSLPATGAFTARARLLAGRI
                     DTRALVEEGRASLGVGGALTFVFRYGAVVSFVEEEAPVQRIDAALRPYLRDPVSAIEV
                     ETADIAIARQGADKIGGEGQILLADAGEARLTLVAIVLARSVVLSRDEVLVSQAFERI
                     APLVEGLRATGRTRFPIRQAMRLVGDVLAARHRVMASVQADERPDVLWDNPELDRLYA
                     RLEAEYELDERAEVLGRKFEALGDFAEVLLNIIQDKRAFRLELAIIFLIMFEIVLALM
                     NMHWW"
     gene            complement(1435..2331)
                     /locus_tag="VFF98_04385"
     CDS             complement(1435..2331)
                     /locus_tag="VFF98_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU62898.1"
                     /translation="MTAPCLTPDRPSLLPAPDWGALLPYAALAGSVLSVCLGTSFAKQ
                     LFPLIGAPATVAYRVGFSALVLMLVFRPWRLSLRRAEGLAVLRYGAAIGLMNFCFYMA
                     LRSIPLGLALAIEFLGPLSVSLIHARRPAHFAAVGLAVAGLSLLLPRPQGGPALDPVG
                     VGFALAAAVFWGSYILFGKRTGQLPAGAAVALGMGTAACLVVPVGVATAGAAMFSLPL
                     AAMGLVAAVLSSAVPYSLEMVALKRLPARHFGVLMSVEPAVGALAGALLLGEHLAGLQ
                     WLAVGLVVAASAVSVLAAGKAH"
     gene            2416..2898
                     /locus_tag="VFF98_04390"
     CDS             2416..2898
                     /locus_tag="VFF98_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU62899.1"
                     /translation="MAETVTLDPFDRRIIEIVRRHNLEPARAIAEAVGLSESAVLRRL
                     RRLRAAGVIVADVALIDPARLAPSITIHVLVELNQTGLRMEQAFARKLEGRAEVVGAW
                     NVTGRTDFLLTVAVPSIEAYQRFSDEVLASDDNVRGFETLVSLREIVRHDPLRAGLPA
                     "
     gene            complement(2895..4283)
                     /locus_tag="VFF98_04395"
     CDS             complement(2895..4283)
                     /locus_tag="VFF98_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU62900.1"
                     /translation="MSPLILRADHIITMDPARTVLADGAVLVTQGGRIGAVGPAAEVI
                     AAHPGVAVRALGPRVLMPGLINTHAHSGLLRGTAEGLPVWEWLRQYIDPMHRVVNPHE
                     AEVAARLCYAESLLAGTTTIVDMWRHMEGAAAAASDLGLRAVLVPYVAEHPVHDYFET
                     LDSNEALIRRWHGGAGGRVQVWVGMEHMFYAVPEAWQRAMAMARDHGVGFHTHSNESR
                     FDVEEAGRRYGLRPIAAMERFGLLDAPRTLLAHCVWLDDAEIAILAARGIGVAHNPVS
                     NMKLASGAAPVERLRQAGVAVGLGTDGEKENNNFDMFEEMKTASLLAKLSGLDAGALD
                     AWSVCAMATIDGARALGMADEIGSLEVGKAADLIALRCDTPRMTPLLGGAGGNLHHNI
                     VHAVRGGDVDLTMVAGRVVVEKGRLVDGDLAAIIGAVNAAVPGLFARRAAWLAAHPTI
                     DALSGDAPGSDA"
     gene            complement(4391..5317)
                     /locus_tag="VFF98_04400"
     CDS             complement(4391..5317)
                     /locus_tag="VFF98_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62901.1"
                     /translation="MSGPRLLGRAAAYFEEVARCGSIRRAAESLHISASAIDRQIIDL
                     EADLGVALFERSPRGLRLTAAGEILLGAVRRWRRDLARSLTQIDDLRGLRRGEVTIGL
                     VEGCIDFLAGGIGSFRAAYPGIAYRLLVAGGPRVCEMVAAQEVDFALVFNPPPANHAL
                     HVEKTLIYQIGAVVRADDPLAARAEIAFAEIAAHPLILPDDSLSLSPVVQQAAARLIG
                     SPLRGCAVANSVAGMKALVRAGVGVGVMYAVDACEEIASGAFRFLPFSEPNLPVSALS
                     VITASGRSLAMPARLMVGHFAGVMAGAGVPVV"
     gene            complement(5373..6497)
                     /locus_tag="VFF98_04405"
     CDS             complement(5373..6497)
                     /locus_tag="VFF98_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HZU62902.1"
                     /translation="MRQVALYTHERTFWHATGMQALFLPVGGWVQPPAGGVGADTPES
                     KRRILSLLSASGLVEQMRTPRAQPATRADLARVHTQRYLDAFAGASAAGWGDLGDVAP
                     FGPGAYEIACLSAGLAKAAVADVLSGEVERAYALCRPAGHHCLPDRSMGFCLLCNIPI
                     AIEAARASHGLGRVAVVDWDVHHGNGTQAVYYERGDVLTISLHQERCFPPGYTGEADR
                     GAGAGLGANLNIPLPPGCGHAAYLAALDEIVLPALERFAPELIIIASGLDANGLDPLA
                     RMLLHSGTYRAMTARLCALADRLCGGRLVVVHEGGYSEAYVPFCGHAVAEALLETASP
                     VVDPAQAMIEAQQPAGATLAFQLETVAQLAQGWRESWKSG"
BASE COUNT         1107 a         2446 c         2150 g          850 t
ORIGIN      
        1 agaaggccgc ccgcctcgcc ttccgggcgc cgccgctgcg ccgttcgggg ccgctgtcgg
       61 cggcagagcg ggccgcggcc atcgcgctgg ccggcgtggc cgaggaacag gtggcagaag
      121 ccttccatgg cgacaacggc cccggctggc aggtgctgcg gctgcattcg gccgccgccg
      181 tgctggccgc ccgcccggcg ccccatgccc ccgccaacac cgacatcggc ctgctgggcc
      241 cctggccggc cgatgcgcca gaggccgatg gcgccgcctg ggaactgcgc ggcctgttca
      301 gcctgcccac cggcgcgctg ggcgaagacc cggttaccgg cagcctcaac gcggcggtgg
      361 cctgccatct gtttgccacg ggccaggcag cgggcgatta tgtggcggcg cagggccgct
      421 gcgtgggcgc tgccggccgc atccatgtgc accgcgatgc agaaggcatc tggatcggcg
      481 gcgatgtggc gatggtggcg cgcggcgggc agctggccgg ctgagccatg ccgcctacca
      541 ccagtgcatg ttcatcagcg ccagcacgat ctcgaacatg atgaggaaga tgatcgccag
      601 ctccagccgg aaggcgcgct tgtcctggat gatgttgagc agcacctcgg caaaatcgcc
      661 cagcgcctcg aacttgcggc ccagcacctc ggcgcgctcg tccagctcgt attccgcttc
      721 cagccgggca tagagccggt ccagctcggg attgtcccag agcacgtcgg ggcgttcgtc
      781 ggcctgcacg ctggccatca cccggtggcg cgcggccagc acatcgccca ccagccgcat
      841 cgcctggcgg atgggaaagc gcgtgcgccc ggtggcgcgc aggccctcca ccagcggcgc
      901 gatgcgctcg aaagcctgcg acaccagcac ttcatcgcgt gaaagcacca cgctgcgggc
      961 cagcacgatg gccaccagcg tcagccgcgc ctcgccggca tcggccagca ggatctgccc
     1021 ctcgccgccg atcttgtcgg ccccctggcg ggcaatggcg atgtcggccg tctccacctc
     1081 gatggcgctc accggatcgc gcagataagg gcgcagcgcc gcatcgatgc gctgcaccgg
     1141 cgcctcttcc tcgacaaagc tgaccaccgc gccatagcgg aacacgaaag tgagcgcccc
     1201 gcccaccccc agcgaagcgc gcccctcttc caccagcgcg cgcgtgtcga tccgcccggc
     1261 cagcagccgc gcgcgggcgg tgaaggcgcc ggtggccggc aggctgccca gcatcaccac
     1321 atccgggtgg cccacatgga aacccgcggc ttgcggcaag agggcggcgc tggcgatcat
     1381 ggctggcttt ctgctggctt tggcccctgc cctagcacgg ccgctgtgac agcatcaatg
     1441 cgccttgccc gcagccagca ccgagaccgc cgaggccgcc accaccagcc ccaccgccag
     1501 ccattgcagc ccggccagat gttcgcccag cagcagcgcg ccggccagcg cgcccaccgc
     1561 cggctcgacg ctcatcagca cgccgaaatg gcgagcgggc aggcgcttca gcgccaccat
     1621 ctccagcgaa tagggcaccg cgctcgataa caccgcggcc accagcccca tcgccgccag
     1681 cggcagcgag aacatcgccg ccccggccgt ggccacgccc accggcacca ccaggcaggc
     1741 cgccgtgccc atgcccagcg ccaccgccgc gcccgccggc aactggccgg tgcgcttgcc
     1801 aaagaggatg tagctgcccc agaacaccgc ggccgccagc gcaaagccca cccccaccgg
     1861 atccagcgcc ggcccgccct gcggccgcgg cagcagcagc gacaggcccg ccaccgccag
     1921 ccccaccgcg gcgaaatgcg ctggccttcg cgcatggatc agcgacacgc tgagcggccc
     1981 gagaaactcg atcgccagcg ccagccccag cgggatgctg cgcagggcca tgtagaaaca
     2041 gaagttcatg aggccgatcg ccgcgccata gcgcagcacc gccagccctt ccgcccgcct
     2101 gagcgacagc cgccaggggc ggaacaccag catcagcacc agcgccgaaa agcccacccg
     2161 ataggccacc gtggccggcg cgccgatcag cggaaacagt tgtttggcaa aggaagtgcc
     2221 caggcacacc gacagcaccg agccggcaag cgcggcatag ggcagcagcg ccccccaatc
     2281 cggggcaggc agcagcgagg ggcgatcagg ggtcaggcaa ggcgcggtca tggcctggct
     2341 atagccaagg agggctggcg gatatcggcg aaatcaggca tgatttgccg gaaacaggcg
     2401 gatacgtgaa acagaatggc ggaaacagtc accctcgatc ccttcgaccg gcgaatcatc
     2461 gagatcgtcc ggcggcataa tctggaaccg gcgcgcgcca tcgccgaggc ggtcggcctg
     2521 tcggaaagcg cggtgctgcg gcggctgcgg cggctgcggg cggcgggcgt gatcgtggcc
     2581 gatgtggcgc tgatcgatcc ggcgcggctg gccccttcca tcaccatcca tgtgctggtg
     2641 gagctgaacc agaccgggct gcggatggag caggcctttg cccgcaagct ggaagggcgc
     2701 gccgaggtgg tgggcgcctg gaacgtgacc gggcgcaccg atttcctgct gacggtggcg
     2761 gtgccctcga tcgaggccta ccagcgcttt tccgacgagg tgctggcgtc tgacgacaat
     2821 gtccgcggct ttgaaacgct ggtcagcctg cgcgagatcg tgcgccacga tcccttgcgg
     2881 gcggggctgc ccgcctaggc gtcgctgccc ggcgcatcgc cactcaatgc gtcaatggtg
     2941 gggtgggcgg ccagccaggc cgcgcggcgg gcaaacaggc ccggcacggc cgcgttcaca
     3001 gcgccaatga tcgcggccag atcgccatct accaaccggc ctttctccac caccacgcgg
     3061 ccggccacca tcgtcagatc gacatcgccg ccgcgcaccg catggacgat attgtggtgc
     3121 agattgccgc ccgccccgcc cagcagcggg gtcatgcgcg gggtgtcgca gcgcagggcg
     3181 atcagatcgg ccgccttgcc cacttccagc gagccgatct cgtcggccat gcccagcgcc
     3241 cgcgcgccat cgatcgtggc catcgcgcac accgaccagg catccagcgc gccggcatcc
     3301 agccccgaga gcttggccag cagcgaggcc gtcttcatct cctcgaacat gtcgaaattg
     3361 ttgttttcct tctcgccatc ggtgcccagc cccaccgcca cgccggcctg gcgcagcctt
     3421 tccaccggcg ccgcgccgct cgccagcttc atgttgctga caggattatg cgccacgcca
     3481 atgccgcgcg cagccaggat ggcgatctcg gcatcgtcca gccacacgca atgggccagc
     3541 agcgtgcgcg gggcatccag caggccaaag cgttccatcg cggcgatcgg ccgcaggcca
     3601 tagcggcgcc cggcttcctc cacatcgaag cggctttcat tgctgtgggt gtgaaaaccc
     3661 acgccatgat cgcgcgccat cgccatcgcc cgctgccagg cctcgggcac ggcatagaac
     3721 atgtgctcca tgcccaccca cacctgcacg cggccgcccg ccccgccgtg ccagcggcgg
     3781 atcagcgcct cgttgctgtc cagcgtctcg aaataatcat gcacggggtg ctcggccaca
     3841 tagggcacca gcaccgcgcg caggcccaga tcgctggcgg cagcggccgc gccttccatg
     3901 tggcgccaca tgtcgacgat ggtggtggtg ccggccagca gcgattcggc atagcacagc
     3961 cgcgcggcca cttcggcctc atgcgggttc accacccggt gcatcggatc gatatactgg
     4021 cgcagccatt cccacaccgg cagcccttca gccgtgccgc gcagcaggcc ggaatgggca
     4081 tgggtgttga tcagccccgg catcagcacc cgcggcccca gcgcccgcac cgccacgccc
     4141 ggatgggcag cgatcacctc ggccgccggg cccaccgcgc caatccgccc gccctgcgta
     4201 accagcaccg cgccatcggc cagcacggtg cgcgccggat ccatggtgat gatgtgatcg
     4261 gcgcgcagga taaggggcga catgggcaaa atctccgtga aagcaggccc ggcctagccg
     4321 ccccccgtgc gcgcttccat cagcatttgt gcagcatgcc gttcgcaaaa agcgagcgcc
     4381 ggcgcccccc tcacaccacc ggcacgccgg cgcccgccat caccccggcg aaatggccca
     4441 ccatcagccg ggccggcatc gccaggctgc gccccgatgc ggtgatgacc gacagcgccg
     4501 agaccggcag gttgggctcg ctgaagggca ggaagcgaaa ggcgccgctg gcgatctcct
     4561 cgcaggcatc gacggcatac atcaccccca cccccacgcc cgcgcgcacc agcgccttca
     4621 tccccgccac gctgttggcc acagcgcagc cgcgcaaggg gctgccgatg aggcgcgcgg
     4681 ccgcctgctg caccaccggg ctcagcgaca ggctgtcgtc cggcaggatc aggggatggg
     4741 cggcgatttc ggcaaaggcg atctcggcgc gcgcggccag cggatcatcc gcgcgcacca
     4801 ccgcgccgat ctggtagatc agcgtttttt ccacatgcag cgcatggttg gccggcggcg
     4861 ggttaaacac cagcgcaaaa tccacctcct gcgcggccac catctcgcac acgcgcgggc
     4921 cgccggccac cagcagccga taggcaatgc ccggataggc ggcgcgaaag ctgccaatgc
     4981 cgccggcgag gaaatcgatg cagccctcga ccaggccgat cgtcacctcg ccccggcgca
     5041 ggccgcgcag gtcgtcgatc tgggtgaggc tgcgggccag atcgcgacgc cagcggcgca
     5101 ccgcgcccag caggatctcg cccgccgctg tcaaccgcag gccgcgcggg ctgcgctcga
     5161 acagggccac gcccagatcc gcctccagat cgatgatctg ccggtcgatc gccgaggcgg
     5221 agatgtgcag gctttcggcc gcccggcgga tcgatccgca acgggccact tcctcgaaat
     5281 aggcggcggc gcggccaagc aggcgtggac cggacatgct ccctcatttt gcgaacgcaa
     5341 ggacgcctgt ttgccgccaa tccgccgcgc tgtcatccgc ttttccagct ctcccgccag
     5401 ccctgcgcca gctgcgccac cgtttccagc tggaaagcca gggtagcgcc ggccggctgc
     5461 tgcgcctcga tcatcgcctg cgccggatcg accacgggcg aggcggtttc cagcagcgct
     5521 tctgccaccg catggccgca aaagggcaca taggcttccg aatagccccc ttcatgcacc
     5581 accaccagcc gcccgccaca cagcctgtcg gccagcgcgc acagccttgc cgtcatcgcc
     5641 cgataggtgc ccgaatggag cagcatgcgc gccagcggat ccagcccgtt ggcatccaac
     5701 ccgctggcga tgatgatcag ctcgggcgcg aaacgctcga gcgcgggcag aacgatctcg
     5761 tccagcgccg ccagataagc cgcatgcccg cagcccggcg gcagcggaat gttgaggttg
     5821 gcccccagcc ccgcgcccgc gccgcgatcc gcctcgccgg tgtagccggg cggaaagcag
     5881 cgctcctgat gcagcgagat ggtcagcaca tcgccgcgct cgtaatagac cgcctgggtg
     5941 ccgttgccgt ggtgcacgtc ccagtccacc accgccacgc ggcccaggcc atggctggcg
     6001 cgggccgcct caatggcgat ggggatattg cacagcaggc aaaaacccat ggagcgatcg
     6061 ggcaggcaat ggtggccggc cgggcggcac agcgcatagg cccgctccac ctcgcccgac
     6121 agcacatcgg ccaccgccgc cttggccagg cccgcagaaa ggcaggcgat ctcgtaggcg
     6181 ccggggccaa acggggcgac atcgccaaga tcgccccagc cggcagcgct ggccccggca
     6241 aaggcatcca gatagcgctg cgtgtgcacg cgggcgagat cggcgcgagt ggccggctgc
     6301 gcgcgggggg tgcgcatctg ctcgacaagg cccgaggcgc tgagcagcga gaggatgcgg
     6361 cgcttggatt ccggcgtatc ggcccccacc ccgcccgccg gcggctgcac ccagccgccc
     6421 accggaagga acagggcctg catgccggtg gcgtgccaga aggtgcgttc gtgcgtgtaa
     6481 agggcaacct ggcgcatgaa aactctcttg ttgcgagaag acataaggag ggggcggcgg
     6541 ctttcgggga acc
//
LOCUS       DASRYE010000080         7320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_56622,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000080 DASRYE010000000
VERSION     DASRYE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56622"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..789
                     /locus_tag="VFF98_04410"
     CDS             259..789
                     /locus_tag="VFF98_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62903.1"
                     /translation="MSFKDTLLALMTPLLAPFLTLALAGGAHAEGAAKPAATAPVAEA
                     DTAEWPEAWFEIFKLAPGKQEAFVRRIALEDEAEKAVGRPPIELYFHEDGADWDVLLL
                     KPEPARPLTEAEEAVLKAKRRALGMPSGPAYFVDIRQLIAAHTDTKAEGPISAAQWLA
                     RLDSWRLAHPAPAPGR"
     gene            971..3133
                     /locus_tag="VFF98_04415"
     CDS             971..3133
                     /locus_tag="VFF98_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62904.1"
                     /translation="MARSKNLAAIASLPLLAAGLGWANQALAADADSAGKDDTIIVTA
                     QKANQTHVEQGGSLGVLGNLSGLDAPFAVKSYDSALILNQQPLTLGQVLDNDPSVRTT
                     YGYGNASEQYVIRGFALYGEDIAIDGLYGITPRQLQSPELYDQIQVLNGASAFLFGAA
                     PGGSALGGTVNLEPKRATMKPINRVTVNYISGSQVGGSFDFGRRFGSDGALGVRINGS
                     GRWGDDAINDEYRASQVLGLGVDFKRGNLRLSLDLAYQREKVRHMRPMVQVYDVSLPL
                     APSDPAENYGQPWNYTTLRDIFGLFKAEYDITKDFTLYGQFGAKNSHEAGQYQSLYIY
                     NSAAGTGVATGSFIPRKDDNMAEQVGIRARLVTGPISHKLSLGISAIQQSERTAFGFG
                     NFGGTATSLYTPVAVPVPDFSYFPGGNISNPYPITRLQQFSMFVSDTMSVADGKLLVT
                     VGARRQKIHQDNYGYYDSGALDSYYAASKTTPVIGVVVHPVKALSVYFNRIAGLSQGG
                     TAPINSAYSNSGQSFAPYSTTQYEVGSKLEIGHYSASLAFYTVEKPNYASAVQGTQYL
                     YVVDGVSRHRGIEFTFNAEPVKGLRLISGFSVVDAKLHGSSEDGKTELGVPKFTANAN
                     VEWDMPYIRGFTLTGRVIHTASQFYDEANTMAIPEWTRYDLGARYVFTAHDLPVTLRF
                     NVDNVANKSYWASTYGTYGGQLVQGMPRTFKASATIDF"
     gene            complement(3404..6757)
                     /locus_tag="VFF98_04420"
     CDS             complement(3404..6757)
                     /locus_tag="VFF98_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU62905.1"
                     /translation="MARASILAGVALLALAAPLPAAAHHRSPLAGLADPDQPPHAPPP
                     RHGHRHPAAATPPQAPSQRLPMAPARELAYTVHSGTWTSLDVAPDGQTILFDMLGHLF
                     AMPAAGGTARQLSFGLGFDSQPTFSPDGRFIAFVSDRSGADNLWVARADGSQPRQITR
                     NDDDTAFVSPAWSADGQALIVSRYRADYNHVELWRIGLDGASQLIVPVRNGPDSHRPD
                     WQSSLGATVSPDGRHVYFARHTGGLNYDEQDSWSILRRDLATGEEVAVVAPPAGRSAK
                     HYTYFRPQLSPDGTQLAYITRTDGGNELRLRNLADGTDRLIATRLDPDALEAAIWLDI
                     APRYAFTPDGKAIILARGGQILRIALDQPAAPTPLPFTAAMRVAVGPSTRQTLRESDG
                     PVNARLIQAPAVSPDGRQVAFMALGRLYVAAVAPGATPRLLATGADPVAQPAWSPDGR
                     QIAFVSWNEAAGGQLWRMTADGSAPPLRVSALPAYYTTPVFTPDGSGLLVLRSAAAAR
                     QKAVFEFGNARPDELLRFALADVDPQAGKVIARGNFGGRPHFAGDPGEVLLLDGRGLT
                     GVRLADGARRPLAEVLGPGYYFEDGDVPVSDILLSPDRRWLLVQSVEQAYLLPMPQGG
                     KPGPAHLDITEPAAHALRLTREGADYLQWAGSDIVWSLGSSLFRLGADKAAAGLTTPD
                     LVLPLPVSAARAVPQGTLVLRGGRVLTMAHGDAVIERADIVITGNRIAAIGPEGSLPI
                     PAGATLRDVSGKTILPGFVDEHDHIGEVRRGLLSTDDWGLKARLAYGVTTSFDPSTLS
                     IDMLSYQDMLDAGLMLGPRLRSTGPAIFSMERITSLDDARAVLARYRDFYRLGNIKEY
                     RTGNRRVREWLAMATREAGLQPTTEGALSLKLDLSQIIDGYANNEHALAAVPLGEDVI
                     GLLAAMHTGYSATLSVTNSGPPAGEWFGAVEDPASDAKLRHFWPQSAIEAKLDAHDWI
                     PLRRFAFPAIAGEVAKAAAGGAVVGMGAHGEVPGIGFHWEMEAHQLGGMAPMAILHAA
                     TAGSAEVIGRLADLGTLEPGKLADLVVLDEDPRGDVRKARSLALVMRGGTLYRAETLD
                     ELWPAAQPAAPASFPAHRPGETEHWLGAP"
     gene            complement(6757..>7320)
                     /locus_tag="VFF98_04425"
     CDS             complement(6757..>7320)
                     /locus_tag="VFF98_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU62906.1"
                     /translation="LPLAIETERESDIRTALALAARLKLRLVLVGAAEGWRAADAIAA
                     AHVPVVLNPFANLPTSFDELGSRLDNAAILAAHGVTVAIGKAGGGIEESYNAGLALRE
                     GAGLAVANGLPHATGLAAIITAPRAIWGLPAPTLTAGAPADIVVWDGDPLEPATNARA
                     VIIEGQEANLANRQRALAEHYLGGERR"
BASE COUNT         1180 a         2405 c         2591 g         1144 t
ORIGIN      
        1 ccatggggca gccggaggaa ggcaaggcag ggggctggtg tgccgcgcgc ttggctctgt
       61 ggcaccctcc agaattagcg ggaaggctgg gccaaggaaa gagtcgccac cgggccatgg
      121 gcgccgggcg tcacggggcc agctggcccg gtgaagctgc gcgaactggc ggtttaccgc
      181 aaggggcgcc gcggcgcgaa tggggcctgc cttgcggatc caccgcaggt tcgcacaggt
      241 tcaaaaaagg gggtttgcat gtctttcaag gatacgctgc tggcgctgat gacgccgctt
      301 ctggcgccgt ttctgacgct ggccctggcc ggcggggccc acgccgaagg ggcggcaaag
      361 cccgccgcca ccgcccctgt ggccgaggcc gacactgccg aatggcccga ggcctggttc
      421 gagatcttca agctggcgcc gggcaagcag gaggccttcg tgcgccggat cgcgctggag
      481 gatgaggccg aaaaagccgt gggccgcccg cctatcgagc tgtatttcca cgaggatggc
      541 gccgattggg atgtgctgct gctgaagccc gagccggcca ggccgttgac ggaggccgag
      601 gaagcggtgc tgaaggccaa gcgccgggcg ctgggcatgc ccagtggccc ggcctatttc
      661 gtcgacatcc gccagctgat cgccgcccac accgacacca aggccgaagg gcccatttcg
      721 gccgcgcaat ggctggcccg gctggatagc tggcggctgg cccatccggc gccggcgccg
      781 ggccgctagg gcagccgccg gtggccctga ggcgggcgcc aattggtccc gcaaagcttg
      841 ccggattgga tgtttcgcat tgaccaatgc ggagagactt tggccaccag cacaacaatg
      901 agggtcgtcc ggctggcatg ggatatccgc cagcgtcagc cgggccagga agataataag
      961 ggggtttact atggcaagat cgaagaattt ggcggccatt gccagcctgc cgctgctggc
     1021 tgcaggcttg ggctgggcaa atcaggcgct ggccgccgat gccgacagcg ccggcaagga
     1081 cgacacgatc atcgtcaccg cccagaaggc caaccagacg catgtggaac agggcggttc
     1141 gctgggcgtg ctgggcaacc tgtcggggct ggatgcgccc tttgcggtca agagctatga
     1201 ttcggcgctg atcctcaacc agcagccgct cacccttggg caggtgctcg acaacgaccc
     1261 ctcggtgcgc accacctatg gctatggcaa tgcttccgag cagtatgtga tccgcggctt
     1321 tgccctttat ggcgaggata tcgccatcga cgggctctat ggcatcacgc cgcgccagtt
     1381 gcagtcgccc gaactgtatg accagatcca ggtgctgaac ggcgccagcg ccttcctgtt
     1441 tggcgcggcg cccggtggct cggcgctggg cggcacggtg aacctggagc ccaagcgcgc
     1501 cacgatgaag ccgatcaacc gcgtgacggt gaactatatc tcgggcagcc aggtgggcgg
     1561 ctcgttcgat tttggccgcc gcttcggttc ggatggcgcg ctgggcgtgc gcatcaacgg
     1621 ttctggccgc tggggcgatg atgccatcaa cgatgaatat cgcgcctcgc aggtgctggg
     1681 cctgggcgtg gatttcaagc gcggcaatct gcgcctgtcg ctcgatctgg cctatcagcg
     1741 cgagaaggtg cgccacatgc gcccgatggt gcaggtgtat gatgtgagcc tgccgctggc
     1801 cccttcggat cccgcggaaa actatggcca gccgtggaac tacaccacgc tgcgcgatat
     1861 cttcggcctg ttcaaggccg agtatgacat caccaaggac ttcaccctct acggccagtt
     1921 cggcgccaag aacagccacg aggccggcca gtaccagtcg ctctacatct acaacagcgc
     1981 ggccggcacc ggcgttgcca ccggctcctt catcccgcgc aaggacgaca acatggccga
     2041 gcaggtgggc atccgcgccc gccttgtcac cgggccgatc agccacaagc tgagcctggg
     2101 catctctgcc atccagcaga gcgagcgcac cgctttcggc ttcggcaatt ttggcggcac
     2161 ggccaccagc ctctacacgc cggtggccgt gccggtgccc gatttcagct atttccccgg
     2221 cggcaacatc agcaatccct atccgatcac ccgcttgcag cagttctcga tgttcgtgtc
     2281 ggatacgatg agcgtggccg atggcaagct gttggtgacc gtgggcgccc gccgccagaa
     2341 gatccaccag gacaattatg gctattacga cagcggcgcg ctcgattcct attatgccgc
     2401 ttccaaaacc acgccggtga tcggtgtggt ggtccacccg gtgaaggcgc tgtcggtcta
     2461 tttcaaccgc atcgccggcc tcagccaggg cggcacggcg ccgatcaatt cggcctacag
     2521 caattcgggc cagtctttcg cgccctattc caccacgcag tatgaagtgg gcagcaagct
     2581 ggagatcggc cattattcgg cctctctcgc cttctacacg gtggagaagc cgaattacgc
     2641 atcggcggtg cagggcacgc aatatctcta tgtggtcgat ggcgtctcgc gccaccgcgg
     2701 catagagttc accttcaatg ccgagccggt gaaggggctg cggctgatct cgggcttctc
     2761 ggtggtggat gccaagctgc atggcagcag cgaggatggc aagaccgagc tgggcgtgcc
     2821 caagttcacc gccaatgcca atgtggaatg ggatatgccc tatatccgcg gcttcacgct
     2881 gaccggccgg gtgatccaca ccgccagcca gttctacgac gaggccaaca ccatggcgat
     2941 ccccgagtgg acccgctatg atctgggcgc gcgctatgtg ttcaccgcgc atgacctgcc
     3001 ggtgacgctg cgcttcaacg tcgataatgt agcgaacaag tcctattggg cctccaccta
     3061 tggcacctat ggtggccagc tggtgcaggg catgccgcgc accttcaagg cgtctgccac
     3121 gatcgacttc tagggcattt tcagcaccat ggccgggcgg gcccttttgc ccggccatgg
     3181 tgcgcacgca ggccgcctct ggcggttggc acaagggccg ctttcagcgg ctggcacgaa
     3241 ggccgctttc gggtcgcacc aggggcgcct tcaggggcag gcaggcatcg cttccgggcg
     3301 gtgcctgcct gccctttttg cggcccggca aaggcgcgcc caaaagaagg gccgcaagcg
     3361 cccaagcgcc gcggcccaag gtgcaggaaa acaaaccgcg gttctagggc gcgcccagcc
     3421 agtgttccgt ctcgccgggg cggtgggcgg ggaagctggc gggggcggcg ggctgtgctg
     3481 cgggccacag ctcgtccagc gtctcggcgc gatagagcgt gccgccgcgc atcaccaggg
     3541 ccaggctgcg ggccttgcgt acatcgccgc gcgggtcttc atccagcact accagatctg
     3601 ccagcttgcc gggctccagc gtgccaaggt cggccaggcg gccgatcact tcggcagagc
     3661 ctgccgtggc cgcgtggagg atcgccatgg gggccatgcc gcccagctgg tgcgcttcca
     3721 tttcccagtg gaagccgatg ccgggcactt cgccatgggc gcccatgccc accaccgcgc
     3781 cgccggcggc ggccttcgcc acctcgccgg cgatcgccgg gaaggcaaag cggcgcagcg
     3841 ggatccagtc atgcgcgtcc agcttggcct cgatggcgct ttgcggccag aaatggcgca
     3901 gcttggcatc gctggccggg tcttccaccg cgccaaacca ctcgcccgcc ggcggcccgc
     3961 tgttggtgac ggacagcgtg gcggaatagc ccgtgtgcat ggccgccaga aggccgatca
     4021 catcctcgcc cagcggcacg gcggccagcg catgctcgtt gttggcatag ccatcgatga
     4081 tctggctgag atcgagcttg aggctgagcg cgccctcggt ggtgggctgc agcccggcct
     4141 cacgcgtggc catcgccagc cattcgcgca cgcggcggtt gccggtgcga tattccttga
     4201 tattgcccag cctgtagaaa tcgcgatagc gcgccagcac cgcgcgggca tcgtccagcg
     4261 aggtgatccg ttccatcgag aagatggcag ggccggtgga gcgcaggcgt ggccccagca
     4321 tcagcccggc atccagcatg tcctgatagg aaagcatgtc gatgctgagg gtggaggggt
     4381 cgaagctggt ggtcacgcca taggccaggc gcgccttcag cccccagtcg tcggtggaaa
     4441 gcaggccccg gcgcacttcg ccgatatggt cgtgctcgtc gacaaagccg ggcaggatgg
     4501 tcttgccgct gacatcgcgc agggtggcgc cggcggggat gggcaggctg ccctcggggc
     4561 cgatggcggc aatgcggttg ccggtgatga cgatatccgc ccgctcgatc accgcatcgc
     4621 catgggccat cgtcaacacc cggccgccgc gcagcaccag cgtgccctgc ggcacggcgc
     4681 gggccgcgct gaccggcagc ggcagcacga gatcgggcgt ggtgaggccc gcggcggcct
     4741 tgtctgcccc caggcggaac aggctgctgc ccagcgacca gacgatatcg ctgccggccc
     4801 actggaggta atcggcgcct tcccgcgtca gccgcagggc atgggcggca ggttcggtaa
     4861 tgtcgagatg cgcagggccg ggcttgccgc cctgtggcat cggcagcaga taggcctgct
     4921 ccaccgattg caccagcagc cagcgccggt cggggctgag gaggatgtcg ctcaccggca
     4981 catcgccatc ctcgaaataa tagcccgggc ccagcacttc ggccagcggg cggcgggcgc
     5041 catcggccag gcgcacccct gtcagcccgc gcccgtcgag cagcagcacc tcgccgggat
     5101 cgccggcgaa atgcgggcgc ccgccaaaat tgccgcgggc gatcaccttg cctgcttgag
     5161 gatccacatc ggccagggca aagcgcaaca gctcgtcggg ccgggcattg ccgaattcga
     5221 acaccgcctt ctgccgcgcc gccgccgccg agcgcagcac cagcaggccg ctgccatcgg
     5281 gcgtgaacac gggcgtggtg taataggcag gcagggccga gacgcggagc ggcggcgcgc
     5341 tgccatctgc ggtcatgcgc cacagctgcc cgccggccgc ctcgttccag ctgacaaagg
     5401 cgatctgccg gccatccggg ctccatgccg gctgggcgac aggatcggcg ccggtggcca
     5461 gcaggcgcgg cgtggcaccc ggcgccactg ccgccacata gagccggccc agcgccatga
     5521 aggccacttg ccggccatcg ggcgagacgg cgggggcctg gatcagccgg gcgttcaccg
     5581 gcccgtcgct ttcgcgcagg gtctgccggg tggaggggcc aacggccacg cgcatggccg
     5641 cggtgaaggg cagcggcgtg ggggcggccg gctggtccag cgcgatgcgc aggatctggc
     5701 cgccgcgcgc aaggatgatc gccttgccat cgggcgtgaa ggcatagcgc ggggcgatat
     5761 ccagccagat cgccgcttcc agcgcatcgg gatcgagccg ggtggcgatc agccggtcgg
     5821 tgccatcggc caggttgcgc aggcgcagct cgttgccgcc atcggtgcgg gtgatatagg
     5881 ccagctgcgt gccatcgggc gaaagctgcg ggcggaaata ggtgtagtgt ttggccgagc
     5941 ggccggccgg cggggccacc accgccactt cctcgccggt tgccagatcg cgccgcagga
     6001 tcgaccagct gtcctgctcg tcatagttga ggccgccggt gtggcgggcg aaatagacat
     6061 ggcggccatc gggcgagacg gtggcgccaa ggctgctctg ccagtcgggg cggtggctgt
     6121 cggggccatt gcgcaccggc acgatcagct ggctggcgcc atcgaggccg atgcgccaca
     6181 gttccacatg gttgtaatcg gcccgatagc ggctgacgat cagcgcctgg ccatccgcgc
     6241 tccaggcggg cgagacgaag gcggtgtcat cgtcgttgcg ggtgatctgg cggggctggc
     6301 tgccatcggc gcgggccacc cacagattgt cggcgcccga gcgatcggag acgaaggcga
     6361 tgaagcggcc atcgggcgag aaggtgggct ggctgtcgaa gccgaggccg aagctgagct
     6421 ggcgggcggt gccccctgcg gccggcatgg cgaagagatg gcccagcatg tcgaacagga
     6481 tggtctggcc atcgggcgcc acatccagcg aggtccaggt gccggaatgg acggtgtagg
     6541 ccagctcgcg ggcaggcgcc atcggcaggc gctggctggg ggcctggggc ggggtggcag
     6601 cggccgggtg gcggtggcca tggcgaggcg gcggggcgtg gggcggctgg tcggggtcgg
     6661 caaggccggc cagcgggctg cggtggtggg cggcggccgg cagcggggct gccagggcca
     6721 gcagcgccac gccggcaagg atagaagcgc gggccatcag cggcgctccc cgcccagata
     6781 atgctcggcc agcgcccgct ggcggttggc gagattggct tcctgcccct cgatgatcac
     6841 cgcgcgggca ttggtggccg gctccagcgg atcgccatcc cacaccacga tatcggccgg
     6901 ggcgccggcg gtgagcgtgg gcgccggcag gccccagatg gcgcgcggcg cggtgatgat
     6961 ggcggccagc cctgtggcat ggggcaagcc attggccacc gccagcccgg ccccttcgcg
     7021 cagggcaagg ccggcattgt agctttcctc gatgccgccg cccgccttgc cgatcgccac
     7081 cgttacgccg tgcgcggcca ggatcgcggc attgtcgagc cggctgccca gctcgtcgaa
     7141 gctggtgggc aggttggcaa aggggttcag caccaccggc acatgggccg cggcaatcgc
     7201 atcggccgcg cgccagccct cggccgcgcc caccagcacg aggcgcagct tcagccgcgc
     7261 ggccagcgcc agagcggtgc ggatgtcgct ttcgcgctcc gtctcgatgg ccagtggcag
//
LOCUS       DASRYE010000081         9130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_161958,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000081 DASRYE010000000
VERSION     DASRYE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161958"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFF98_04430"
     CDS             complement(<1..483)
                     /locus_tag="VFF98_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZU62907.1"
                     /translation="MDCERIALNHSFGGEQGVYTHQSSATGTAMTFSVFVPPHAAGAR
                     LPVLFYLSGLTCTHANVTEKGEYRRACAEQGVIFVAPDTSPRGEDVADDEAYDFGKGA
                     GFYVNATEAPWAAHYRMRDYIEAELPALIAAQFPMADMSRQGITGHSMGGHGALTVAL
                     R"
     gene            776..3145
                     /locus_tag="VFF98_04435"
     CDS             776..3145
                     /locus_tag="VFF98_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HZU62908.1"
                     /translation="MARLEHRRQRIRNIAVVALSTSALLAPAAAYAQCSQSGTTYTCT
                     GTTTSFSTNSTANAAVTVNSGATVIAPVTIQGDSSTLTNAGSITNTGAYYAVTMGTNA
                     TVTNTGSITSTGTSSGSGAIYVGDGSTVTNSGTLTAYSGTPVVTFGTNGTFNNTTSAA
                     TAVTGNIYYGSSTSSVSANGTFKNENVSYGFTGNVYSYGGLTFNNQGLYSGNVYMYMV
                     SNSDSVAFTNGTLTSGGTYDTNTTFTGIYYTQGGGTFVNNGTAYLYTGSTIGSNLAYQ
                     AGNTATVTNNGILYVGTTAAPAQLTIYGNYVQSSSGTLNIAIIPAGASTTSAGTNYSQ
                     LYTTGTATLSGTLNVNVTAGFYKDGTTFNVVDALKGITNNGATFTVNSGSPPIFLNFT
                     SLGVQTVSGTEQAYQVEVTHNGYASTMTSLGGSANQIAVAGAFDYKNSAKTTGLLATA
                     NANPAGDEATLLGQLDVLDLNDAKVALNTLSPEGYLAYASALRDQANAFARQVDLRMT
                     DQNSKHPEDGWWFNFLGAFDTASTASTTGGYRTRDHLFGVYAGYDLSGPHHVIGIAAS
                     ISWDSLHYALNSLTGTNRDFAFAVYGAQNFGALRLSGQLAYNLGHMSATHTTTLGDYT
                     RTGKGKAGENMFKATAQLGYDVKLGGYKLEPFVGIDFNKGQVNSFTETDAGAANLTVG
                     AIKADRTEALFGASVNRDHGVFRPYLKALYREKISGSDATTVSAYLDGDNSTAFSVNG
                     LGLGKGEFDANAGINWVFDDAGALFVGYQGTYRSSYQSHGISLGLRLEF"
     gene            3326..4126
                     /locus_tag="VFF98_04440"
     CDS             3326..4126
                     /locus_tag="VFF98_04440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HZU62909.1"
                     /translation="MSGLKSTAAARRLSVRLARDAEDLAAVQRLRWQVFFAEMGANGT
                     SFDPTERFDRDAYDEVCDHLLVIDEDRDWPESVVGTYRLLRESVAERHGGFYSAGEFD
                     LTRLIHREKEEGDTRELLELGRSCVLPAYRTSATISMLWRGIGEYIAANRIAMMFGCA
                     SFTGTDPDLYAPALSYLYHQHLAPEERRPVVHPGKGVAMERLARGSYDERRAMLQLPP
                     LVKGYMRVGAKFGNGVYIDHDFNTVDVCVLMPVDLIADRYLTRFSAAA"
     gene            complement(4181..4966)
                     /locus_tag="VFF98_04445"
     CDS             complement(4181..4966)
                     /locus_tag="VFF98_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZU62910.1"
                     /translation="MGRALVMGWRGFRAALGSALLVIFAWMWLAWPHKATKRAAVVRA
                     WRALLWGFRIDVVVKGKAAALPGTLYVANHVSWTDIVVIGRVLDAPFVAKGDVRGWPI
                     IGTLTTAYGCVYIDRERRAGAGRQAEDVGAHLARSGELVLFAEGTTGLGNEVLPFRTS
                     LFALVPQNEAGEARVQPITLRYTARDGSPLSPADRREVAWIGDDEMLPHMKALARRGG
                     LRVEVWFEEPVTGSAAGRGRKDLAAASRARIAERLASFPENAG"
     gene            complement(5047..6042)
                     /locus_tag="VFF98_04450"
     CDS             complement(5047..6042)
                     /locus_tag="VFF98_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU62911.1"
                     /translation="MPITRTLAGRDVNPVGLGCMNVSWAYAAPPPPEVAARLLHLALD
                     LGYNHLDTARLYGGGTNELLIGEALAGRRQDFFLASKMGLFAEGGKRWVDCRPETIRA
                     ECEKSLKALQTDHIDLYYMHRRDFAVPIEECAGAMGELIAEGKIGGYGLSEMSAATLR
                     AAHAVTPVTAVQTEYSLWTRNPELGVLAATAELGVALVAFSPLARGVLANGVRNPGQL
                     EEKDLRRTHPRFNGENWPTNLALVDAFNAIAGEEGATPAQLALAWVLSRGDHVHVIPG
                     TTNPDHLRENIARADWRPADAVLARIDGLINRHTVAGPRYPAAMQATMDTEEFAD"
     gene            complement(6187..7134)
                     /locus_tag="VFF98_04455"
     CDS             complement(6187..7134)
                     /locus_tag="VFF98_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU62912.1"
                     /translation="MRIALAGAGAFGEKHLDGLKNIPGVEITSIISRTGEQAAAVAAK
                     YGAKHSSTELDDALARDDVDAVILCTPTQMHAAQAIACMKAGKHVQVEIPLADSWADA
                     QAVLAVQQETGKVAMVGHTRRFNPSHQYVHNQITAGAFNIQQMDVQTYFFRRTNINAK
                     GEARSWTDHLLWHHAAHTVDLFAYQAGRIVAANAVEGPIHPVLGIAMDMSIQLKSETG
                     AICTLSLSFNNDGPLGTFFRYIGDTGTYIARYDDLVDGRENAIDVSQVDVSMNGIELQ
                     DREFIAAIREGREPNSSVQKVFDCYRVLGELEVQLNAAK"
     gene            complement(7198..8040)
                     /locus_tag="VFF98_04460"
     CDS             complement(7198..8040)
                     /locus_tag="VFF98_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HZU62913.1"
                     /translation="MARITAGVASSHVPLLGVAVDQNKTQDPYFKPIFDGYEWTKAWE
                     KAEKPDVVVLVYNDHASFFDMKIVPTFAIGTAERFGICDEGWGPRPVPEVEGHADLGW
                     HIAQSLILDDFDMTVVSEMDVDHGLTVPLSMMFGQPEAWPCKVIPLAVNVVTYPVPSG
                     NRCWLLGEAIRRAVESYPEDLNVQIWGTGGMSHQLQGPRAGLLNAEWDNRFMDLLIGE
                     SEEARWIEHIEYLRETGSEGIEMVMWLIMRGALGKATKCLHRHYHIPCSNTAIGHLVL
                     EPAE"
     gene            complement(8042..8461)
                     /gene="ligA"
                     /locus_tag="VFF98_04465"
     CDS             complement(8042..8461)
                     /gene="ligA"
                     /locus_tag="VFF98_04465"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HZU62914.1"
                     /translation="MTDQAPTQDIHAYLAEFDDIPGTRVYTAARARKGYWLNQFAMSL
                     MKPENRERWRADEAAYLADWPMSEEQKAAVLARDYNRMLDLGGNIYFLSKVFSTDGLS
                     FLQAVSTMTGMSVEDYSAMMNAGGRSPEGVRSIKGKC"
     gene            complement(8519..>9130)
                     /locus_tag="VFF98_04470"
     CDS             complement(8519..>9130)
                     /locus_tag="VFF98_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU62915.1"
                     /translation="GCNLNPDPGGGHFKHPPLTNDYWFPLYEKMVELDVPAMIHVSGS
                     CNPAMHATGAYYIAADTIAFMQLLEGDLFSRFPTLRFIIPHGGGAVPYHWGRYRGLAD
                     MLKKPSLDTHLMNNIFFDTCVYHQPGINLLADVIDNKNILFGSEMVGAVRGIDPTTGF
                     YFDDTKRYVDALDISDAERRAIFEGNARRVFPRLDAQLKARGL"
BASE COUNT         1595 a         3099 c         2865 g         1571 t
ORIGIN      
        1 gcgcagcgcc accgtcaacg cgccatggcc gcccatggaa tggccggtga tgccctgccg
       61 gctcatatcc gccatgggga actgcgcggc gatcagcgcg ggcagttccg cctcgatata
      121 gtcgcgcatg cggtaatggg ccgcccaggg cgcttctgtg gcgttgacat aaaagcccgc
      181 gcccttgccg aaatcatagg cctcgtcatc ggccacatcc tcgccgcgcg gagacgtgtc
      241 gggcgccacg aaaataacgc cctgttcggc gcaggcgcgg cgatattcgc ccttttccgt
      301 cacattggca tgggtgcagg tgaggcccga cagatagaac agcaccggca gccttgcccc
      361 tgcggcatgc ggcggcacga agacggaaaa ggtcatcgcc gtgccggtgg cgctgctctg
      421 gtgggtatat accccctgct ccccgccgaa gctgtggttc agggcaatcc gttcgcaatc
      481 catcctgccg gtctccttgg agcggccctg ccgggccggg cgttcatcct gcaagcgcct
      541 taggttgctg catgcgccgg tgtaaagcga gcgcgcccgg ccgctggcgc tgcagccctg
      601 caacaatttc cgacaccccc tcggcccacc gcagatgtcg ccgctattgc cgcatttttg
      661 cgttgccttc gcgtcacaaa ccgggcagca gacgggccga cgcccggaag ggggctcgta
      721 tcaggatcat ttcgcccgca accgggcgac aaggcaaacg aggacgtaac gggacatggc
      781 caggcttgag catcgcaggc agcgtatccg caatattgcc gttgtcgcgc tgtcgacatc
      841 ggcgctgctg gcgccggcgg ccgcctatgc ccagtgcagc caatcgggca ccacctacac
      901 ctgcaccggc accaccacct cgttcagcac caacagcacc gccaatgccg ctgtcacggt
      961 gaattcgggc gccacggtga tcgctcctgt caccatccag ggtgattcca gcacgctcac
     1021 caacgccggc tcgatcacca acaccggcgc ctattatgcc gtgacgatgg gcaccaacgc
     1081 caccgtcacc aacaccggct cgatcacctc caccggcacc tcgtcgggct cgggcgcgat
     1141 ctatgtgggc gatggcagca ccgtgaccaa cagcggcacg ctgaccgcct atagcggcac
     1201 gccggttgtc accttcggca ccaacggcac gttcaacaac accaccagcg ccgccaccgc
     1261 cgtcaccggc aacatctatt atggttccag caccagctcg gtgtcggcca acggcacgtt
     1321 caagaacgag aacgtgtcct atggcttcac cggcaacgtc tattcctatg gcgggctgac
     1381 cttcaacaac cagggcctct attcgggcaa cgtgtacatg tacatggtgt cgaacagcga
     1441 cagcgtggcc ttcaccaacg gcacgctgac ctcgggcggc acctacgaca ccaacaccac
     1501 cttcaccggc atctactaca cgcagggcgg cggcaccttc gtcaacaacg gcaccgccta
     1561 tctctacacc ggcagcacca tcggctccaa cctggcctat caggccggca acaccgccac
     1621 ggtgacgaac aacggcatcc tctatgtggg caccaccgcc gcgccggcgc agctgaccat
     1681 ttatggcaat tacgtgcaga gctcgagcgg cacgctgaac atcgccatca tccccgccgg
     1741 cgccagcacc acttcggccg gcaccaacta cagccagctc tacaccaccg gcaccgccac
     1801 cctttccggc acgctgaacg tcaacgtcac cgccggcttc tacaaggatg gcaccacctt
     1861 caacgtcgtc gatgcgctga agggcatcac caacaacggc gccaccttca cggtgaacag
     1921 cggctcgccg ccgatcttcc tcaacttcac ctcgctgggt gtgcagacgg tgagcggcac
     1981 cgagcaggcc tatcaggtgg aagtgacgca taacggctat gccagcacga tgacctcgct
     2041 gggcggcagc gccaaccaga tcgccgttgc cggcgccttc gactacaaga acagcgcgaa
     2101 gaccaccggc ctgctggcca ccgccaatgc caatccggcg ggcgatgagg ccaccttgct
     2161 gggccagctc gacgtgctgg atctcaacga tgccaaggtg gcgctcaaca cgctgtcgcc
     2221 ggaaggctat ctggcctatg cctcggcgct gcgcgatcag gccaatgcct tcgcccgcca
     2281 ggtggacctg cggatgaccg accagaactc gaagcacccc gaggatggct ggtggttcaa
     2341 cttcctgggc gccttcgaca ccgcctccac cgcttccacc accggcggct atcgcacccg
     2401 cgatcacctg ttcggcgtct atgccggcta tgatctgtcg ggcccgcatc acgtgatcgg
     2461 catcgccgcc agcatctcgt gggattcgct gcattatgcg ctgaacagcc tgaccggcac
     2521 caaccgcgac tttgcctttg ccgtctatgg cgcgcagaat ttcggggcac tgcgcctgtc
     2581 gggccagctg gcctataacc tgggccacat gtctgccacc cacaccacca cgctgggcga
     2641 ctatacccgc accggcaagg gcaaggcggg cgagaacatg ttcaaggcca ccgcgcagct
     2701 gggctatgac gtgaagctgg gcggctacaa gctggagcct ttcgtcggca tcgacttcaa
     2761 caagggccag gtcaacagct tcaccgagac cgacgccggc gctgccaacc tcaccgtggg
     2821 cgccatcaag gcagaccgca ccgaggccct gttcggcgct tcggtcaacc gcgatcatgg
     2881 cgtgttccgg ccctatctga aggcgctcta ccgcgagaag atcagcggca gcgacgccac
     2941 caccgtctcg gcctatctcg atggcgacaa cagcaccgcc ttctcggtga acgggctggg
     3001 cctgggcaag ggcgaatttg atgccaatgc cggcatcaac tgggtgttcg acgatgccgg
     3061 cgcgctgttc gtgggctatc agggcaccta tcgttccagc taccagtcgc acggcatcag
     3121 cctgggcctt cgcctggagt tctgagggcc tggccggcgc ttgcgggggc aggcgccggc
     3181 cggcatcgca ggcctgcaac atgcctgcaa caggggctgc cgggccgggg ggcttgtcgg
     3241 agcggcccct gtctgtcatg aaatcatcac gtcgcagaaa aagatctgtc acaagttctt
     3301 gctagggatg ggcgcaggag gctccatgtc aggtctgaaa tccactgctg cagcgcgtcg
     3361 cttgtctgtc cgcctcgccc gggatgcgga ggatctggcc gctgtccagc gcctgcgctg
     3421 gcaggtgttc ttcgccgaaa tgggcgccaa tggcaccagt ttcgacccca ccgagcggtt
     3481 cgaccgcgat gcctatgacg aggtgtgcga tcacctgctg gtgatcgacg aggaccgtga
     3541 ctggccggaa tcggtggtcg gcacctatcg cctgctgcgc gagagcgtgg ccgaacgcca
     3601 tggcggtttc tattccgccg gcgaattcga cctcacccgc ctgatccacc gcgaaaagga
     3661 agagggcgac acgcgcgagc tgctggaact gggccgttcc tgcgtgttgc cggcctatcg
     3721 caccagcgcc accatctcga tgctgtggcg cggcattggc gaatatatcg cggccaaccg
     3781 catcgccatg atgttcggct gcgcctcttt caccggcacc gatcccgatc tctatgcgcc
     3841 ggccctctcc tacctctatc accagcatct ggcgcccgaa gagcgccggc cggtggtgca
     3901 ccccggcaag ggcgtggcga tggagcgtct ggcccgcggc agctatgacg agcgccgcgc
     3961 catgctgcag ctgccgccgc tggtgaaggg ctatatgcgg gtgggcgcca aattcggcaa
     4021 tggcgtctat atcgaccatg atttcaacac ggtcgacgtg tgcgtgctga tgccggtgga
     4081 tcttatcgcg gatcgctatc tcacccgctt cagcgccgcg gcctgacagc gcgcctgaca
     4141 gcaagcgcgc gggagggggc aaacgccccc tcgccccgtt tcagccggcg ttttcgggaa
     4201 agctggccag ccgctcggca atgcgggcgc ggctggcggc ggccagatcc ttgcggccac
     4261 ggcccgccgc agagcctgtc accggctctt caaaccacac ttccacgcgc agaccgccac
     4321 gccgggccag cgccttcatg tgcggcagca tctcgtcatc gccgatccac gccacctcgc
     4381 gccggtcggc cggcgacagg ggcgaaccat cgcgcgcagt gtagcgcagg gtgatcggct
     4441 gcacccgcgc ctcgccggcc tcgttctgcg gcaccagggc aaacaggctg gtgcggaagg
     4501 gcagcacttc gttgccaagg ccggtggtgc cctcggcaaa cagcaccagc tcgccgctgc
     4561 gggccagatg ggcgcccaca tcctcggcct ggcggccggc gcccgcgcgc cgctcgcggt
     4621 cgatatagac gcagccatag gccgtcgtca gcgtgccgat gatcggccag ccgcgcacat
     4681 cgcccttggc cacgaaaggc gcatccagca cccggccaat caccacgata tccgtccacg
     4741 agacatggtt ggcgacatag agcgtgcccg gcagcgccgc agccttgccc ttcaccacca
     4801 catcgatgcg gaagccccac aagagcgccc gccacgcccg caccacagcg gcgcgcttgg
     4861 tggccttgtg gggccaggcc agccacatcc aggcaaaaat caccagtaag gccgagccca
     4921 gcgccgccct gaagccccgc cagcccatca ccaatgccct gcccatattt gcccaagccc
     4981 ccctatgccg tataacgccg ggcctgacag cacgccccgc cgcccggcgc aagggggtgc
     5041 tggcgatcag tcggcgaatt cctctgtatc catcgttgcc tgcatggcgg cgggatagcg
     5101 ggggccggcc acggtgtggc ggttgatcaa cccgtcgatc cgggccagca cggcgtctgc
     5161 ggggcgccag tcggcgcggg cgatattctc gcgcaggtga tcggggttgg tggtgcccgg
     5221 gatcacatgc acgtgatcgc cccggctgag cacccaggcc agcgccaatt gcgccggcgt
     5281 tgcgccctct tccccggcaa tggcgttgaa ggcatcgacc agcgccaggt tcgtcggcca
     5341 gttctcgccg ttgaagcgcg gatgggtgcg gcgcaggtcc ttctcttcca gctgccccgg
     5401 attgcgcacg ccattggcca gcacgccgcg cgccagcggg ctgaacgcca ccagcgccac
     5461 gcccagctcg gccgtggccg ccagcacgcc cagttcaggg ttgcgcgtcc acagcgaata
     5521 ttccgtctgc acagcggtaa caggggtgac ggcatgggcc gcgcgcaggg tggccgcgct
     5581 catttccgaa aggccatagc cgccgatctt gccctcggcg atcagctcgc ccatggcgcc
     5641 ggcgcattcc tcgatcggca cggcaaaatc gcggcggtgc atgtagtaga ggtcgatatg
     5701 gtcggtctgc agggccttca ggctcttctc gcattcggcg cggatggtct cgggccggca
     5761 atccacccag cgcttgccgc cctcggcaaa caggcccatc ttgctggcca ggaagaaatc
     5821 ctgccgccgc ccggccaaag cctcgccgat cagcagctcg ttggtgccgc cgccatagag
     5881 gcgcgcggta tcaagatggt tgtagcccag atccagcgcc agatgcagca gccttgccgc
     5941 cacttccggc ggcggcgggg cagcataggc ccagctgaca ttcatgcagc caaggccaac
     6001 ggggttcaca tcgcggccgg ccagtgtgcg ggtgatgggc atggcggata tccttggctg
     6061 ataaaaggaa tgcaggggtg tggccccctg catcccataa cgccttcgcg cgcaagccca
     6121 atcaacagct gcggcccgaa ggcggcagcg ccgcgagagc ccaggcggcc ctgcggcgcc
     6181 cggtgtttac ttcgcggcgt tcagctgcac ttccagctcg cccagcacgc gatagcagtc
     6241 gaacaccttc tgcacgctgg aattgggctc gcggccctcg cggatggcgg cgatgaattc
     6301 gcggtcctgc agctcgatgc cgttcatcga cacatcgacc tggctgacat cgatggcatt
     6361 ctcgcggcca tcgacgagat cgtcgtagcg ggcgatatag gtgccggtgt cgccgatgta
     6421 gcggaagaag gtgcccagcg ggccatcatt gttgaacgac agcgacaggg tgcagatggc
     6481 gcccgtctcg ctcttcagct ggatcgacat gtccatggcg atgcccagca ccggatggat
     6541 cgggccctcg acggcattgg cagccacgat cctgcccgcc tgataggcga acagatccac
     6601 cgtgtgggcg gcgtggtgcc acagcaggtg gtcggtccag ctgcgggcct cgcccttggc
     6661 gttgatgttg gtgcggcgga agaaataggt ctgcacatcc atctgctgga tgttgaacgc
     6721 gcccgcggtg atctggttgt gcacatactg gtggctgggg ttgaagcggc gggtgtggcc
     6781 caccatcgcc accttgccgg tttcctgctg gacggccagc accgcctggg catcggccca
     6841 gctgtcggcc agggggattt ccacctgcac atgcttgccc gccttcatgc aggcgatcgc
     6901 ctgggcggcg tgcatctggg tgggggtgca caggatcacc gcatccacat catcgcgcgc
     6961 cagcgcatcg tccagctcgg tggaggaatg cttggcgcca tatttggcgg ccacggccgc
     7021 ggcctgctcg ccggtgcggc tgatgatcga ggtgatctcc accccgggga tgttcttcag
     7081 gccatcgagg tgcttttcac cgaaggcgcc ggcgcccgcc agggcaatgc gaatgggatc
     7141 ggacatgggt gttctccaca agagggaagg ggcggccctg cggccacccc tgtgggatta
     7201 ttctgccggt tcgagcacca gatgcccgat cgcggtgttg ctgcagggga tgtgatagtg
     7261 gcggtggagg catttggtgg ccttgcccag cgccccgcgc atgatcagcc acatcaccat
     7321 ctcgatgcct tcgctgccgg tctcgcgcag atattcgata tgctcgatcc agcgcgcttc
     7381 ctcgctctcg ccgatcagca ggtccatgaa gcggttgtcc cactcggcgt tgagcaggcc
     7441 tgcgcgcggg ccctgcagct ggtggctcat gccgcccgtg ccccagatct gcacgttgag
     7501 atcttccgga tagctttcca ccgcgcggcg gatcgcctcg cccagcagcc agcatcggtt
     7561 gcccgagggc accggatagg taacgacatt gaccgccagc gggatgacct tgcacggcca
     7621 tgcctcgggc tggccgaaca tcatcgagag cggcacggtg aggccatgat cgacatccat
     7681 ttcggaaacg acggtcatgt cgaaatcgtc gaggatcagc gattgggcga tgtgccagcc
     7741 cagatcggca tggccctcca cctcgggcac cgggcgcggg ccccagccct cgtcgcaaat
     7801 gccgaagcgc tcggccgtgc cgatggcgaa agtgggcacg atcttcatgt cgaagaagct
     7861 ggcatggtcg ttgtagacca gcacgacgac atcgggcttc tcggcctttt cccaggcctt
     7921 ggtccattca tagccatcga agatcggctt gaaataggga tcctgggtct tgttctggtc
     7981 caccgccacg cccagcaggg gcacgtggct ggaagcgaca cctgcggtta tccgcgccat
     8041 ctcaacactt ccccttgatc gagcgcacgc cctcgggcga gcggccgccg gcgttcatca
     8101 tcgccgaata gtcttccacg ctcatgccgg tcatcgtcga gacggcctgc aggaagctga
     8161 ggccatcggt cgagaagacc ttggagagga aatagatgtt gccgcccaga tccagcatgc
     8221 ggttatagtc gcgcgccagc acggccgcct tctgctcctc gctcatcggc cagtcggcca
     8281 gataggcagc ctcgtcggcg cgccagcgct cgcggttttc cggcttcatc aggctcatgg
     8341 cgaactggtt cagccaatag cccttgcgcg cgcgcgcggc ggtgtagacg cgggtgccgg
     8401 ggatatcgtc gaactcggcg agataggcgt ggatgtcttg cgtgggggcc tggtcggtca
     8461 tgccacctct cctcattgtg ggcccggcag cgcgcggccg ccgggctcca tcgcattctt
     8521 acaggccgcg cgccttcagc tgggcatcca gccgcgggaa cacccggcgg gcattgccct
     8581 cgaagatggc gcggcgttcg gcatccgaga tatccagcgc atcgacatag cgcttggtat
     8641 catcgaaata gaagccggtg gtgggatcga tgccgcgcac cgcgcccacc atctcgctgc
     8701 cgaacaggat gttcttgttg tcgatcacat cggccagcag gttgatgccc ggctggtgat
     8761 agacgcaggt gtcgaagaag atgttgttca tcagatgcgt gtcgagcgac ggcttcttca
     8821 gcatgtcggc caggccccga tagcggcccc agtgataggg caccgcgccg ccgccatgcg
     8881 ggatgatgaa gcgcagcgtg gggaagcggc tgaacagatc gccttccagc agctgcatga
     8941 aggcgatggt gtcggcggcg atgtaatagg cgccggtggc atgcatggcc gggttgcagc
     9001 tgcccgagac gtggatcatc gccggcacgt ccagttccac catcttctcg tagaggggga
     9061 accagtagtc gttcgtcagc ggcgggtgct tgaagtggcc gccgccggga tcggggttga
     9121 ggttgcagcc
//
LOCUS       DASRYE010000082         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_91985,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000082 DASRYE010000000
VERSION     DASRYE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91985"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFF98_04475"
     CDS             <1..250
                     /locus_tag="VFF98_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZU62916.1"
                     /translation="WRAYDACALIADGARLPDLLVDQGAADGFLDGQLKTPLLEAACQ
                     AAGIPATIRLQQGYDHSYYFISTFMAEHVEWHAQRLKG"
     gene            338..2578
                     /locus_tag="VFF98_04480"
     CDS             338..2578
                     /locus_tag="VFF98_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62917.1"
                     /translation="MAASAYSGTAHASADTAAPAGAGEIVVTAQKREQNVQKVGIAIT
                     ALGKETLAAIGRQDVTALAGQVPDLAVNQYSPTITVFNIRGISQNDFTDAQEAPIAFY
                     EDEVYVASLGAITGMNFDLERVEVLRGPQGTLFGRNATGGLVQFVSAKPTARPEAFLT
                     LTTGSYGQLATEGAVSGPISDAVRARLSFTTNNEGGYIRNTMGPSLGNAKFFALRGQI
                     TADLSPVDRLNIKLQVLRNAHERSGGLYSWVASYPDPNNHLLGDYAANVPGGNPGAAY
                     TGAAQGQDANGYANHSGNPFVQSENRVPFFDRTYWDASARYTHDLGWAELASITDYQH
                     LAKDYGEDTDMSPNDAFNYDTHQRMYQISQEVRLSGRQGGLNWVGGVYWLKIHTANQY
                     QIDSPVTAYPGLGLLNYGGTQDTNSLATFAQLEYAISPKVSVIGGLRYSHDKKHIEFA
                     DYANGVLMFDWATSFPDVAGLRYATYDNWSGKAEIDYKPAGNVLIYASVNRGTKSGGF
                     GTVSSPTPYTDSAAVSEATGRPLVQAIPFGQEVLTNWEGGYKLTLLNHTTHFNGSVFF
                     YNYQGYQAFQNLGINQYISNHQAYVHGAEFDFNARPVAGLYLGAFLTLLSTRVKAIVL
                     PDGTLADRQLPQAPKTSIGWQARYEFPAGPGRLALSTDWKYESGQYFETNNAPVDFQP
                     AHTVGNVRVSYALAEGKYELAAFLNNVTDRHYLVYSLDLSSLLGASTQTYARPRTWGL
                     SLTARY"
     gene            complement(2631..3254)
                     /locus_tag="VFF98_04485"
     CDS             complement(2631..3254)
                     /locus_tag="VFF98_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU62918.1"
                     /translation="MTENDKKPAGTRRRLSPEARREQIVMQTRRLFCDRGHAGFSLRE
                     VARACGVNLSAVQHYFPGKDTLISEMINHSVTAYARDMHALVDSLGHDPTGQLLSTIE
                     YLISLNNEPDSARFFINLWSFAHEDATGAKYLVDLYQPMIDWLMRCIRRLLPDLPEEE
                     IAMKANLILSTADGLLSAGMLGDCYFKDGPKPSPRQYAEKVLQFALK"
     gene            3340..4545
                     /locus_tag="VFF98_04490"
     CDS             3340..4545
                     /locus_tag="VFF98_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62919.1"
                     /translation="MTKWQPGFSLPQHQYIAPEQFRADVDALGRTQWLLADHESRIPE
                     AGDWFTLDWADEQILLIRGEDGIIRGFYNVCRHRGSRLCLGASGQAKMLICPYHAWGY
                     DLQGRLKGAPLMPAAFDKSAHNLKPVHVEVYEGLVFVNFAPGEPPAFAAFTARFDAYF
                     RPFDLKATRIACRHTIPTAANWKLVVENFFECYHCKPSHPTYCAVHDPLKLLALGAGP
                     GSGAAELVAQYAGKLSAWEAKRSKAGQGTGMWADEACTPWLQSAAHAPINEDFQTETL
                     DGRPVGPLLGSLAGFDGGQMAGVFNVLSTVLINNDHAVLFRIVPRGPLQTDFEVIWLV
                     SPAFDMADAAALDRLVEVWSVTAAEDKQITEDNQAGVLSAAYQPGPLSAQEARIGDFG
                     RWYQRLLQA"
     gene            4556..5716
                     /locus_tag="VFF98_04495"
     CDS             4556..5716
                     /locus_tag="VFF98_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62920.1"
                     /translation="MALRTGSELTLMLLVGLLGVYAAPVEINSIILHLHDDPIRAGGL
                     STIELLALGGVAIASGALADRAGPRQVVLGGALIAGLAQSASMAVASLAGLLALRLMA
                     GLGAGLVYGCACRHANGCRRPSAVFGLAMLCANLLFAGILAVLPALDARFGLAGLFAA
                     LAGVTFAAWLLLAALMRPGAGRGPAEVGAGVEAGTRARAAFVLAGAGGLFLASVFLSN
                     LGLSMVWTSASRLGVENGLSQSWIAYALALSTLANVAGAGLAAMAALLCGPRPMLLAG
                     LAAGAVAAVLILFAARPQIYVLALCLYGAAYLFIIPFQITMAMHLSPRGHLSAITGGL
                     GLMSYGCGPLAGGVAAAMGAYRLVGVACALLCLCAALAVVLVREPRLQPALG"
     gene            complement(5750..6655)
                     /locus_tag="VFF98_04500"
     CDS             complement(5750..6655)
                     /locus_tag="VFF98_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU62921.1"
                     /translation="MELRHLRYFLAVAEERNFTRAAARVGIGQPPLSQQIRDLEAEVG
                     TPLFRRLPQGAELTAAGRAFEAAIARIPGQVADAVRAAQRAARGEIGALRVGFTGSAA
                     MNPIVPATIRTFRHRAPDVALTLSESNTTTLIAGLRDGSIDVAFIRPSESDLQGLHAL
                     ALLAEPLLAAVPTGHPALGQDGAAPIDLRALAQDHFILTPRAIGPCLFDAAISLCEAA
                     GFTPRLGQAAPQIASVLSLVAATEGVSIVPASMRQLSLEGVAYREISHVSATVRLSLA
                     HMSSNRSPLVQRFLAVGHDLIATGA"
     gene            6732..7991
                     /locus_tag="VFF98_04505"
     CDS             6732..7991
                     /locus_tag="VFF98_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62922.1"
                     /translation="MKNAALCKGDWAGGHGPATSTADEQDGFRRGSPAYRRASLALFL
                     GGFATFSLLYCVQPLLPEFARDYHVAPAVSALALSLTTGALALTIFLTGAVSQGLPRR
                     PLMFTSMALAAGCNLLAALAPSWPVLLLARLTEGAVLGGVPAVAMAYLAEEVHVGDLG
                     RAMGLYVAGTAFGGMMGRVGMGLLVEYGSWRMAMALLGVLGLLAAAGFAALLPASVRF
                     RARRGSSLRQHATQWARHLRHGDMARLFGIGFILTSVFVTLFNYAGFRLIAPPFRLGQ
                     TGVSLIFLSYILGMVASPWAGQLADRHGRRGPLVGMLLVMGLGAVVTLSGSLVLITLG
                     ILLVTVGFFGAHAIASGWVGELAGAAKGHAASLYLLFYYVGSSITGSSGGWLWQRYGW
                     WGIVALAVVLALGGIWLAQGVKARADA"
BASE COUNT         1371 a         2582 c         2900 g         1611 t
ORIGIN      
        1 ctggcgcgcc tatgacgcct gcgcgctgat cgccgatggc gcccgcctgc ccgatctgct
       61 ggtggaccag ggcgcggccg atggcttcct ggacggccag ctgaagacgc ctctgctgga
      121 agcggcctgc caggcggccg gcatccccgc caccatccgc ctgcaacagg gctacgacca
      181 cagctactat ttcatctcca ccttcatggc agagcatgtg gaatggcatg cgcagcgcct
      241 gaaggggtga atgaaaggac taaaaacgca aaacagggag aattttatgt gcagtataaa
      301 taagggggtg acatatcatt cattgatttt gtgcgctgtg gcggcaagcg catattcggg
      361 cacggcccat gcctctgccg atacggctgc cccggcgggg gcgggcgaga ttgtcgttac
      421 cgcgcaaaag cgcgagcaga acgtccagaa ggtgggcatt gccattaccg cgctgggcaa
      481 ggaaaccctt gctgccattg gccgccagga tgtgacagcg ctggcagggc aggtgcccga
      541 tctggcggtc aaccagtatt cgccgacgat caccgtgttc aacattcgcg gcatatcgca
      601 aaatgatttc accgatgcgc aggaggcgcc catcgccttc tacgaggatg aggtctatgt
      661 cgcctcgctg ggggcgatca cgggcatgaa cttcgacctg gagcgggtcg aggtgttgcg
      721 ggggcctcag ggcaccttgt tcgggcgcaa cgccacaggc gggctggtcc agttcgtttc
      781 ggccaagcca accgcccggc cggaagcctt tctcacgctc accaccggat cctatgggca
      841 gctggcgaca gagggcgctg tcagcgggcc gatcagcgat gcggtgcgcg cgcgcctgtc
      901 cttcaccacg aataacgaag gcggctatat ccgcaacacc atgggcccca gccttggcaa
      961 tgcgaagttc tttgccctgc gcgggcagat caccgccgat ctgtcgccgg tggacaggct
     1021 gaacatcaag ctgcaggtgt tgcgcaatgc gcatgaacgc tcaggtggcc tttacagttg
     1081 ggtggccagc tatccggatc cgaataacca cctgctgggc gattatgcgg ccaacgtgcc
     1141 gggcggcaat ccgggggcgg cctatacggg ggcggcgcaa gggcaggatg ccaacggcta
     1201 tgccaatcac tcgggcaacc ccttcgtgca gtcggagaac cgggttccct ttttcgaccg
     1261 cacctattgg gatgcaagtg cgcgctacac gcatgatctg ggctgggcgg agctggcctc
     1321 gattaccgat tatcagcacc tggccaagga ttatggcgag gataccgata tgagccccaa
     1381 tgatgccttc aattacgata cgcatcagcg catgtatcag atatcgcagg aagtgcgcct
     1441 ctcgggccgg cagggcgggt tgaactgggt tggcggcgtg tattggctga agatccacac
     1501 cgccaatcaa tatcagatcg acagcccggt gacggcctat cccggccttg gccttctgaa
     1561 ctatggcggc acgcaggaca ccaactccct ggccaccttt gcccagctgg aatatgcgat
     1621 cagccccaag gtgtcggtga ttggcggcct gcgctactcg cacgataaaa agcacataga
     1681 attcgccgat tatgccaatg gcgtgctgat gttcgactgg gcgacatcct tcccggacgt
     1741 ggcggggctg cgctatgcca cttacgacaa ctggtcaggc aaggccgaga tcgactacaa
     1801 gccggccggc aatgtgctga tctatgccag tgtcaaccgc ggcaccaagt ccggcggttt
     1861 tggcacggtg tcctcgccca cgccctatac cgacagcgcc gctgtttcgg aggccacagg
     1921 ccggccgctg gtgcaggcca tcccctttgg ccaggaagtg ttgaccaatt gggaaggggg
     1981 ctacaagctc acgttgctca accacaccac ccatttcaac ggttcggtat ttttctataa
     2041 ttaccaaggg tatcaggcat tccagaacct tggcatcaac cagtatatct cgaaccatca
     2101 ggcctatgtg catggcgccg agttcgactt caacgcgcgg ccggtggccg gcctctatct
     2161 gggggctttc ctcaccctgc tctccaccag ggtgaaggcg atcgtgttgc ccgatggcac
     2221 gctggccgat cgccaactgc cccaggcgcc gaaaacctcg atcggctggc aggcgcgcta
     2281 tgaatttccg gccggccccg ggcgcctcgc cctgagtacc gactggaagt atgaatccgg
     2341 ccagtatttc gagacgaaca acgcccctgt tgatttccag cccgcgcata cggtgggcaa
     2401 tgtgcgggtg agctacgcgc tggccgaggg caaatatgag ctggccgcct tcctcaacaa
     2461 tgtcaccgac cgccactatc tcgtttacag cctcgacctg tcgagcctgc ttggcgcgtc
     2521 tacgcaaacc tacgcccgcc cgcgcacatg ggggctgagc ctgaccgcca gatattgatc
     2581 gtgacgccgg agcagggcca tgcagatggc attgctccgg cccatgcgcc ttacttcagg
     2641 gcaaattgca gcaccttttc ggcatattgc cggggcgagg gcttgggccc atccttgaaa
     2701 tagcaatcgc ccagcatccc ggcagagagc agcccatcgg ccgtcgacag gatgaggttg
     2761 gccttcatgg cgatctcctc ctcgggcagg tccggcagga ggcgccggat gcatcgcatc
     2821 agccagtcga tcatcggctg gtacaggtcg acaagatatt tcgcgcctgt cgcatcctcg
     2881 tgggcaaacg accacaggtt gatgaaaaaa cgcgcggaat caggctcgtt gttgagcgat
     2941 atcagatatt ctatggtcga cagcaattgc ccggtcggat cgtggcccag gctgtccacc
     3001 agcgcatgca tgtccctcgc ataggccgtc accgagtggt tgatcatctc ggagatgaga
     3061 gtgtccttgc cgggaaaata atgttgcacc gccgacaggt tcacgccgca ggccctggcc
     3121 acctcgcgca gggaaaagcc ggcatggccg cgatcgcaaa acagccggcg cgtttgcatg
     3181 acgatctgtt cgcgccgggc ttcgggagac aggcggcggc gcgtgccggc cggcttttta
     3241 tcattttcgg tcatgtctgt tctttaggcc gaaatgcggc ttgtcaaaac catcctttgc
     3301 agcttcatat aggtcaagtg acctttaagg agatgcggca tgacaaagtg gcagccgggc
     3361 ttcagcctgc cccaacacca gtatattgcg ccagaacagt tccgggccga tgtggacgcc
     3421 ctgggccgca cgcaatggct gctggcagac catgaaagcc gcattcccga agccggcgac
     3481 tggttcacgc tggattgggc agacgagcag atcctgctga tccgcggcga ggatgggatc
     3541 atccgcggtt tttacaatgt gtgccgccat cgtggatcgc gcctttgcct tggcgccagc
     3601 gggcaggcaa agatgctgat ctgcccctac cacgcctggg gctatgatct gcagggccgg
     3661 ctgaagggcg ccccgctgat gcccgccgca ttcgacaaat ccgcccacaa cctcaagccg
     3721 gtgcatgtcg aggtgtatga ggggctggtg tttgtcaatt tcgcgccggg cgagccaccc
     3781 gcgtttgccg ccttcaccgc gcgcttcgat gcctatttcc ggccgttcga cctcaaggcg
     3841 accaggatcg cctgccgcca caccattccc actgcggcga actggaagct tgtggtcgaa
     3901 aatttcttcg aatgctatca ctgcaagcca tcgcacccca cctattgcgc ggtgcatgat
     3961 ccgctgaaat tgctggcgct gggggccggg cccggttcgg gcgctgcgga acttgtcgcg
     4021 caatatgccg gcaagctttc cgcctgggaa gcaaagcgca gcaaggcggg ccagggcacc
     4081 ggcatgtggg cggatgaggc gtgcacgccg tggctgcaat cagcggccca tgcgcccatc
     4141 aacgaggatt tccagaccga aacgctggat ggcaggccgg tggggccgct gctgggcagc
     4201 ctggccgggt tcgatggcgg gcagatggcg ggcgtgttca atgtgctgtc cacggtgctg
     4261 atcaacaatg atcatgccgt gctgttccgc attgtgccgc gcgggcccct gcagaccgat
     4321 ttcgaagtga tctggctggt cagcccggct ttcgacatgg cggatgcggc cgcgctcgat
     4381 cggcttgtcg aggtgtggtc cgtcacggcc gccgaggaca agcagatcac cgaggacaat
     4441 caggccggcg ttctttccgc cgcctatcag cccgggccct tgtctgccca ggaagcccgg
     4501 atcggcgatt tcgggcgctg gtatcaacgg ctgctgcaag cctgaggagg tgggcatggc
     4561 gcttcgcacc ggatcggaac tgacgctgat gctgctggtt ggcctgctcg gggtctatgc
     4621 ggcgccggtc gagatcaaca gcatcatcct gcacttgcac gatgatccga tcagggcggg
     4681 ggggctgtcc acgatcgagt tgctggcgct gggcggtgtg gcgatcgcca gcggggccct
     4741 ggccgatcgg gccgggcccc gccaggtggt gctgggcggc gcgctgatcg cggggctggc
     4801 ccagagcgcg tcgatggcgg tggccagtct ggctggcctg ctggcgctgc ggctgatggc
     4861 tggcctgggg gccgggctgg tctatggctg cgcctgccgg catgcgaacg gctgcaggcg
     4921 gccctctgcg gtgtttggct tggccatgct gtgcgccaac ctgctgtttg ccggcatatt
     4981 ggcggttctg ccggctcttg atgcgcgctt tggcctggcc ggcctgttcg ccgctttggc
     5041 cggcgtgacc tttgcggcat ggctgttgct ggcggcgctg atgcggcccg gggcggggcg
     5101 cgggcctgcc gaagttggtg ccggtgttga ggccgggact agggcgcggg cggcttttgt
     5161 gctggcgggg gcggggggcc tgtttctggc ttcggtgttc ctgtcgaacc tgggcctttc
     5221 gatggtgtgg acatcggcca gccggctcgg ggtggaaaac ggcctgtcgc aaagctggat
     5281 cgcctatgcg ctggcgctgt cgaccctggc caatgtggcg ggggcggggc tggcggcgat
     5341 ggcggcgctg ctgtgcgggc caaggccaat gttgctggcc gggctggcag cgggggcggt
     5401 ggcggcggtg ctgatcctgt ttgccgcccg gccgcagatc tatgtgttgg ccctgtgcct
     5461 ctatggcgcg gcctatctgt tcatcatccc gttccagatc accatggcga tgcatctgag
     5521 cccgcgcggc catctgtcgg caataacggg cgggttgggg ctcatgtcct atggttgcgg
     5581 cccgctggcc ggcggcgttg cggcagcgat gggggcctat cgcctggtgg gcgttgcctg
     5641 tgcactgctg tgcctctgcg cggctttggc tgtcgtgctt gtgcgcgagc cccggctgca
     5701 gccggcgttg ggctgatcgg gcggcctgcc atatgtggcc tggcgccagc tacgcgccgg
     5761 tggcgatcaa atcatgcccc acggccagaa aacgctggac aaggggcgag cggttgctgc
     5821 tcatatgcgc cagcgacagc cgcacagtgg cagagacatg gctgatctcg cgataggcca
     5881 ccccttcgag cgagagctgg cgcatggagg ccggcacgat cgatacccct tcggtggctg
     5941 ccaccagcga cagcacagag gcgatctgcg gcgcggcctg ccccagccgg ggcgtgaaac
     6001 ctgccgcttc gcacaggctg atcgccgcat cgaacaggca ggggccaatg gcgcgcgggg
     6061 tcaggatgaa gtggtcctgc gccagggccc tgaggtcgat gggggcggcg ccatcctgcc
     6121 ccagcgccgg gtgcccggtg ggcaccgctg ccagcagcgg ttccgccagc agcgccaggg
     6181 catgcaggcc ctgcagatcg ctctcgctgg gccggatgaa ggcgacatcg atgctgccat
     6241 cgcgcagccc ggcgatcagc gtggtggtgt tgctttcgct cagcgtcagc gccacatcgg
     6301 gcgcgcgatg gcggaaggtg cggatggtgg ccggaacgat cgggttcatc gccgccgatc
     6361 cggtaaagcc cacgcgcagg gccccgatct cgccgcgcgc cgcgcgctgg gccgcgcgca
     6421 cggcatcggc cacctggccg ggaatgcggg cgatggccgc ctcgaaggcc cggccggcgg
     6481 cggtcagctc ggcgccctgg ggcaggcggc gaaagagcgg cgtgcccacc tctgcctcaa
     6541 gatcgcggat ctgctggctg aggggtggct gaccgatccc cacccgggcg gccgcgcggg
     6601 tgaagttccg ctcctcggcc acagcgagaa aatagcgcag gtgacgcagt tccatgccat
     6661 ctccaaaagc tatcgataat gcctctacca tatattggac caaccggagg ggccggtcca
     6721 tatgggcgcg catgaaaaat gctgcactct gcaagggcga ttgggccggt gggcacgggc
     6781 ctgccaccag cacggctgat gaacaggatg gcttccggcg gggcagcccg gcctatcggc
     6841 gcgccagcct ggcgctgttc ctgggtggct ttgccacctt ctcgctgctc tattgcgtgc
     6901 agccgctgct gcctgaattt gcgcgtgatt atcatgtcgc accggcggtc agcgcattgg
     6961 cgctatcgct gacgacaggg gcgctggcgt tgacgatttt cctcacgggc gctgtttcgc
     7021 agggcctgcc gcggcgcccg ctgatgttca cttccatggc gctggcggca ggctgcaacc
     7081 tgctggcggc gctggcgccc agctggccgg tgctgttgct ggcgcggttg accgagggcg
     7141 cggtgctggg gggcgtgccg gcggtggcga tggcctatct ggccgaagag gtgcatgtgg
     7201 gcgatctggg ccgcgccatg gggctttatg tggcgggcac ggcctttggc ggcatgatgg
     7261 ggcgggtcgg catggggctg ctggtcgaat atggctcgtg gcgcatggcg atggcgctgc
     7321 tgggcgtgct ggggctgctg gcggcggcag gctttgcggc gctgctgccg gcttcggtac
     7381 gtttccgcgc caggcggggg agcagcctgc gccagcatgc cacgcaatgg gcccggcatt
     7441 tgcggcatgg cgatatggcg cgcctgttcg gcatcggctt catcctcacc agcgtattcg
     7501 taacgctgtt caactatgcc ggcttccgcc tcatcgcgcc gcccttccgc ctggggcaga
     7561 cgggggtcag cctgatcttc ctcagctata ttctgggcat ggtggcatcg ccctgggctg
     7621 ggcagctggc cgaccggcac ggccgccgcg ggccgctggt gggcatgctg ctggtgatgg
     7681 ggctgggggc ggtggtcacg ctttcgggat cgctggtttt gataacgctg ggcatattgc
     7741 tggtaaccgt gggctttttc ggcgcgcatg cgatcgccag cggctgggtg ggtgaattgg
     7801 cgggggcggc caaggggcac gctgcctcgc tctatctgct gttctattat gtcggctcca
     7861 gcatcaccgg gtcgagcggc gggtggctgt ggcagcgcta tggctggtgg gggattgtgg
     7921 cgcttgccgt ggttctggcg ctgggcggga tctggctggc gcaaggcgtg aaggcgcgtg
     7981 cggatgcgtg atcaccgcgc ccggtcctgc cggccggcca cgcaccttga ggcatgctgt
     8041 gccaaacggg gacaaggcgc ggcagggcaa ggcctgggta agagcgcggc ggcccactgg
     8101 ccagaacctg ccggaacgat cgcccggatt gcggcaatta cgggccaaag cctttagatt
     8161 atcaggcatt atgcccctcg ggcggcgcgg cgccggctgt gcggggcgcg cccttcggcg
     8221 ctcaaaacaa acaggaaacc gcttggcaat ggagcggata tgcgccgccg gtccgcccgg
     8281 gcagacagtc ttggcaaacc ctatcttaac ccttttaact catagcggaa aatgaattgc
     8341 cctgggtgtc ccgctgcggg gcgccggggt ggccggcctt ccttggggat gaatgcaatg
     8401 ccaaccgtga aatccaccct gtgggccctg gcggccgtgg cggtgctggc gctgtcgggc
     8461 tgtg
//
LOCUS       DASRYE010000083        40982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_216572,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000083 DASRYE010000000
VERSION     DASRYE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 40982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40982
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216572"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFF98_04510"
     CDS             <1..281
                     /locus_tag="VFF98_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU62923.1"
                     /translation="AKGFWKKRPKDIAGIGLVYARVNPRTTDRERELLAAGKSATVQT
                     YEASGEVYYGFQFGPLMVRPNVQYINHVGGSSRYSDAIVGGCTIRLTV"
     gene            complement(326..2200)
                     /locus_tag="VFF98_04515"
     CDS             complement(326..2200)
                     /locus_tag="VFF98_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU62924.1"
                     /translation="MINAIATVSLKGTLQEKLEAAASAGFRAVEIFENDLIGAPQRPA
                     EIRRMMADLGLACVLYQPFRDFEGMPGALRQRAFDRAERKFDLMGELGTDRILVCSNC
                     SPQSLGERQRIIDDFAELGERAGRRAIHVGYEALAWGRHIYDHRDVWEIVKAVDHPHV
                     GIILDSYHSLSRRIPSDSIRAIPGHKITFVQLADAPLLDMDLLYWSRHFRNLPGQGGF
                     DLPAYVAEILRTGYDGPLSLEIFNDRFRSHSPRMVAEDGYRSLEYVRDAARRLLGQPT
                     TMPAAQKITGTEFVEFTAAGEEAQRLADTFASLGFAQEGSHRTKKVTRWRQGAVNLVI
                     NAEPRGFANTYRTVYGTSICAIGVRVADVEAVLARAAALGIRRFTPEGRPGRLAMPAL
                     RGVGGSLVYLIGEDASESLWAREFDGPHQPIDTAAPALSGIDHLASVVHNDEFLSWQL
                     YWRALFGLEALSEQDVIDPSGLVHSQALQTADGAFRVTINGSDARETLSARFLAQGMT
                     GGFQHIAFATADAAATSAALAARSACLLEIPANYYDDLAARFGFSQAETEAMQAGHLL
                     FDADAAGHRYTQLYSRAFDRRFFFEFVERGDGYTGLGAPNAGVRLAAQTRFRPAEPVD
                     "
     gene            2511..4994
                     /locus_tag="VFF98_04520"
     CDS             2511..4994
                     /locus_tag="VFF98_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU62925.1"
                     /translation="MGQSVPAAVVWGAAWALATGPGLAGGAAAAPAGDVLAPEEARVI
                     LVTAAAAGKTAQDSALAVSQVDPQAIARFTPRSQAEALRLIPGLNLQDTAGPGGNANI
                     GVRGIPVSTGGAEYVALQEDGLPVVLFGDMLFGNNDYWLRLDNNVARVEAVRGGSAST
                     FASQAPGAVINYVSNTGERAGGGFSLSKALDYRETRIDADYGGPLGDHLRFHIGGYAI
                     TGGGPLHYGFAAEHGYQIKANLTRSFADGGGYVRISLKRLDDREPTDQNMPTIATLAG
                     NTITGFANLPGIDARQFTPIGLYNASFPMLNGSGQLANVPAQGIHPVATALGAEVQRR
                     LGAHLTFTNRFRWTAMKGQFAFFFSGVRATSTVAANGASLRYAAGPALGGTYAGALVN
                     AGTYVNTSMDDMGSLANDLVLGGQASLGRLGLAVHLGWFHMRQKIAAHWAVANGYLSA
                     TSSANPVPLDLFAADGTQLAANGIADYVDHWGDNGAASQRHYRLAYTDDAPYGELAAD
                     IGRLSLDASLRVDLLRATGAVYQSARASGTLAVSDALGTALLPYALPSATPGTLLAYG
                     RSYASWSAGGLYRLGAGTNLFMRASRGGRFNADRVAVNPLNFTAAGGLSAGGAAVAVN
                     YVAQQEVGLKRAGGAGAFAYHLEATFFRAQVVEHNYDFTAPTRGLSPFVDALYHAHGL
                     ELSGEVRRGGFVLAGYVDYTVARDLARNLPAYALPRWNVLLAPAYDAGVAAAGFSLSG
                     QSAFYIANQRVTAPGTFFVNAFVALRPRARVEIGLNANNLFNALGYRANNAGLGPPGE
                     QGVLAGNQAIFNNSIVAGRSLTAALRYRF"
     gene            complement(4982..6361)
                     /locus_tag="VFF98_04525"
     CDS             complement(4982..6361)
                     /locus_tag="VFF98_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU62926.1"
                     /translation="MAQPLPAATGPAHKRRLSLLRILEMNLGFFGLQFAFGLQQGQMA
                     PILSWLGAGEARLPMLQLAGPVTGLLVQPLVGALSDATCWRLGRRLPYVLAGAVLTSL
                     GLALMPFSTSLLMAASLLWLIDAGANTTMEPYRAYIADRLDAGAQPTGFLVQAAFTGL
                     AQSLAYLTPSLLLYQLGVSGSASAHHLPAITRASFLVGAVLSLATVTWSVWRVPEEPL
                     PPADRLPAGAPLGAIWNALRTILRALCAMPAPMRQLAAMSLFQWYGAMIYWSYVALAI
                     GRTAYATADKTSAAFRAAVLASQQLGAVYNLVACAAGFAMVPLARRLGAGRLHRAAMV
                     AMGLGLLALPLAGAGSNAAPPLFSALALAELGRHLLLLGPALGLGLGWASMMGLPPLL
                     VARAVPRQQIGVYMGLYNGMIVVPMLLHALSFGWVYRVVLGGDPARAIALAGMLMLAA
                     ALATPAQKR"
     gene            complement(6466..7104)
                     /locus_tag="VFF98_04530"
     CDS             complement(6466..7104)
                     /locus_tag="VFF98_04530"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HZU62927.1"
                     /translation="MTTPQHALNTALARCPLVAILRGVRPDEVEAIGEALVEAGFTLI
                     EVPLNSPDPLESISRLARRLGPAAVVGAGTVLTTGDVAAVAAAGGQLIVSPDSNPAVI
                     RASVAAGLVSLPGFFTPTEGFAALAAGAHGLKLFPAEGASPAYLKAQRAVLPKGLPVL
                     AVGGVTPDNLGAWMAAGANGAGLGSALYAPGLSAGEVGARAAAFVAALGKAE"
     gene            complement(7101..7970)
                     /locus_tag="VFF98_04535"
     CDS             complement(7101..7970)
                     /locus_tag="VFF98_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HZU62928.1"
                     /translation="MNGQLIAIDWGTTNRRAYLLDGDGTVLHTRRDAMGLLSVPAGGF
                     ADEVAAIRVDLGDLPVLLAGMVGSARGWVNVPYLPCPAGLADLAGALHWVEAGRTAIV
                     PGLADPAGDVMRGEEVQLLGAVAAGLAPDNALLCQPGTHCKWARMAEGRVAGFATAMT
                     GELFALLKAHSLLAEFLTGEVADGEAFRAGVRAGLAGRLTSHLFRVRGSALLGLRKVE
                     DSAAYTSGLLIGHDVAGAALREGDPVHILADPQLGALYASAVHLAAGQPVLLDSHAGF
                     IAGITRIWKASQA"
     gene            complement(7972..9549)
                     /locus_tag="VFF98_04540"
     CDS             complement(7972..9549)
                     /locus_tag="VFF98_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HZU62929.1"
                     /translation="MTLSAIDTAMVVAYGCFIFALAQWVSRSKPGEAENSAGYFLAGR
                     ALPWWAIGASLIAANISAEQIVGTAGSGYAIGLAISSYEWMAAATLLIVGKFFLPVFL
                     KNRITTMPEFLEARFGPTIRTVMALFWLALYIFVNLTSILWLGSVAVTQVAGVNQDVA
                     LAGLGLFALAYQIRGGLKAVALTDIVQVTLLVIGGLVVSGMALGQLGAGAGIAAGFAT
                     LVARVPDHFHMILSPGNPHYKDLPGLSVLIGGMWIANLSYWGFNQYIIQRTLAAKSLA
                     EAQRGIVFAAFLKLLMPMIIVLPGIAAVLLTPNLAKPDQAYPAMMHLLPSGLLGLVFA
                     ALTAAIIASTASKINSIATIFTLDLYRRKIAPAASEAQLVRVGRIAACVATALGILAA
                     RPLLGGLDQAFQYIQEFSGFVTPGITVIFLTGLFWPRATEAGALAGAVASVVLSALFR
                     FGGIAALAAVPFMNRMGIVFAASLALTVVVSLARPAPAREGPGIMAGVHFGTSRGFAF
                     ASALVVAILVVLYAVWW"
     gene            9636..10358
                     /locus_tag="VFF98_04545"
     CDS             9636..10358
                     /locus_tag="VFF98_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZU62930.1"
                     /translation="MDSDADDGRGGGLGRNLTYGLLDHLGRQIVTGAFETTPFPTEAE
                     LSEQHGVSRSVTREAVKMLTAKGLLTARPRQGTSVQPVASWNLFDADVLRWLLERKFS
                     VDLLAQFNQLRAAIEPAAAALAAEAADARALALIEAGFARMEAADRGEDDGLDADIAF
                     HLAILRASGNPFFMQFRELVATALRTSIRFTNRIVGRTADLAAHGAVKEAICARDPAR
                     AQQAMAAIIGDVMSLIAEQRAG"
     gene            complement(10394..11266)
                     /locus_tag="VFF98_04550"
     CDS             complement(10394..11266)
                     /locus_tag="VFF98_04550"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZU62931.1"
                     /translation="MAEWTILPRPAPGGPRRDRLGEGITCVAAQNALYWVDILGQRVH
                     RFGLADNAYAEWQMPEMTGWLLERANGQGFIAGLQSGFHALSLAPFSLTPLAAPEAHL
                     PANRMNDACVDAQGRIWAGTMRADGSGEEGALYRLDPDLAWQRMDAPYAIANGPALSP
                     CGTLLYHTDSAKGLVYRFALQEDGTLGPRQLFLTFPPEWGSPDGMTVDAEGGLWIAHW
                     GAGCVSRFTPEGARERWIDLPASQITKPCFAGPGYTRLFLTSAADGVDEPLAGSVFEV
                     EAGVAGLAPHRFGG"
     gene            complement(11279..13075)
                     /locus_tag="VFF98_04555"
     CDS             complement(11279..13075)
                     /locus_tag="VFF98_04555"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HZU62932.1"
                     /translation="MSESPHKPQLRSRAWFDNPASPDMTALYLERYLNYGLSIEELQS
                     NRPIIGIAQTGSDLSPCNRHHLTLAQRVKDGIREAGGIPIEFPLHPIQETGKRPTAGL
                     DRNLAYLGLVETLYGYPIDGVVLTIGCDKTTPACLMAAATVNIPAIALSVGPMLNGHH
                     KGERTGSGTIVWKARQMLAAGEIDYKGFVQLVASSAPSTGYCNTMGTATTMNSLAEAL
                     GMSLPGNAAIPAPYRDRAESAWHTGRQIVEMVRADRKPSDILTRAAFLNAIRVNSAIG
                     GSTNAPIHLNAIARHVGVELSLADWEEQGADIPLLVNLQPAGEYLGEDYYRAGGVPAV
                     MGELYRAGLLDGSALNANGRTLAENVGSATIEDEAVIRPLSAPLKPAAGLTVLSGNLF
                     EAAVMKKSVISAAFHARYLADPQDPEAFEGHVVVFDGPEDYHARIDDPALGITGRSIL
                     VIRGAGPIGYPGGAEVVNMRPPAALIRAGIDALPCIGDGRQSGTSGSPSILNASPEAA
                     AGGGLALLRTGDVVRIDLRARQVNLLVSEEEMARRRAEEAAAPFTGPDHQTPWHEISR
                     QITGQFAGGAVIESAVKYQRIAQTKGLPRDSH"
     gene            13238..14170
                     /locus_tag="VFF98_04560"
     CDS             13238..14170
                     /locus_tag="VFF98_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU62933.1"
                     /translation="MGGGAVRLGLVGLGKIARDQHVPAIAANPRFALVATASPHGGLD
                     GVAAHADLATMLGAGHGLDAVALCTPPAVRAGLARLALEAGLHVLLEKPPAAGITQVA
                     DLEALAARGGCSLLTTWHSRETAAVDAARAWLAGRRVEAVRVIWREDVRRWHPGQDWL
                     LAAGGFGVFDPAINALSILTHILPEPLALESAELGIPDNREAPMEARLRLRHGGVAAV
                     DCALSILPGGAQQWDIAIDTDAGALLLSEGGHRLAVDGTAQAFDANAEYARLYARFAG
                     MIDAGTRDVDARPLALVADAMMLGGRYRLPAFAF"
     gene            14190..16670
                     /gene="galA"
                     /locus_tag="VFF98_04565"
     CDS             14190..16670
                     /gene="galA"
                     /locus_tag="VFF98_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HZU62934.1"
                     /translation="MLPLRRRAVLAGSLAAGAAMGAAPLARAALPSAGPVLRRATRLA
                     EGWRFHLGHASDPARDFGFGAWQRSFAKPGAELTSAAAADFDDSGWEAVSVPHDWAAG
                     LPYAPPAQVPKGKEDFAAAHGFRAIGRDFPGHSVGWYRLKLPLGKADRGRAVWLEFDG
                     VFRASMVIVNGYHAGGSTSGYAPFRVDIADFLNDDDTPNQLVVRVDASLGEGWFYEGA
                     GIYRHVTLVSAAAQHVPQWGVCVRASPRGTGAEVAVATDITNAAAHPAMVALRQRVIA
                     ADGQVVAESESAPAPLAAGAATTLAQALAFPAARLWSPEDPALYRLETTVLTDGTPVD
                     RVETRFGVRSLRFSGREGFVLNGRPVKLKGVCNHQDHAGVGTAIPDALHAWRLGVMRE
                     MGANAWRSAHNPPAEALLDLCDAQGMMMIAETRLNTTSPEGMDELDRMILSARNHPSV
                     ILWSVGNEEGHQGTPRGARLSAGLVARARALDPTRQTTQAMDSGWYEEGAARVVDVVG
                     FNYRTDQIPAFKAKYPDVPVIGTETASTVATRGAYANDPAAHVVRAYDTEHPWWAANA
                     EGWWPIVAANPGIAGGFIWTGFDYRGEPTPYPAWPSVSSYFGVVDICGFPKDNYHYYR
                     AWWRPDLPQVHLLPHWTWPGREGQPIEVWAHGNCAAVELVLNGRGLGRQAMPANGHLV
                     WQVPYAAGVLEARGLDAAGRLVAQDSRATAGAPAGVRLLASKPRLAADGADVAVIRAE
                     ICDAAGRPVPTARTRLDFALEGPARLLGTGNGNPTDTTPDHAASRPAFNGLAQAIVQS
                     TGATGAIQLAVSGEGVAPARLSLFAHAL"
     gene            16706..17776
                     /locus_tag="VFF98_04570"
     CDS             16706..17776
                     /locus_tag="VFF98_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HZU62935.1"
                     /translation="MSLGLASAALLAASGLHLGADLSFTNEMEACGAHFRENGKSADP
                     FALLKAHGGNIVRVRLWNAPATPYSAYADVLKTITRAHAAGLDVLLDFHYSDDWADGD
                     KQIPPAAWAELSTDAQVQALHDYTAVILDRLAAAGQLPQFVQVGNETNPEMLGGRKGG
                     KIDWARNARLINAGVAAVRAAAKAHGQPIRVMLHIAQPENVIGWFDEATAAGVMDYDI
                     IGISYYAKWSRYNLSGLGKVIDAAHRRYGADVWVVETAYPFTTAAADDTTNLLGADSA
                     IPGYPVSPEGQARYLEDLTRVVVANGGTGVVYWAPDWVSTGCKTRWGTGSSWENAAWF
                     DIAHHEALPALRFLGEDYGAKK"
     gene            complement(17879..20521)
                     /locus_tag="VFF98_04575"
     CDS             complement(17879..20521)
                     /locus_tag="VFF98_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU62936.1"
                     /translation="MIRIPIHTTALSGTLALALVAGAMPARAADATTNTGADTGTDTG
                     ADNPAAQPPAAPDIIVTGLRASLRDAIQAKRSSATITETISSKDIGVLPDVTIADELA
                     RLPGVNATRDRGNDSQASVRGLGPRLVLGLVNGREVASSEPDRNVRWEIYPSETVSSV
                     TVYKSQGADLIAGGVAATIDIRTIRPLDYVGPKLTMRAGALYNDAGIKIPNYDNLGAR
                     GSIQYVTRLTDTLGLSVGGTYQRQRNGYSSFQGWGYNNADAGSSPPTYNGQTVYAPWG
                     AQTEVKALTETRWSTSAALQWKDDEHWDVNADFLYSDVKIDENQFQQWYGGWGDWGGT
                     IPDSLYTAGGFTLAGRDIVAATIPNWGTTVTNVIAKYTEDKNLLATGLNARYRAGDLT
                     VKIDGSYSQARRTNTWAAQEWNVWPQVLSYNTAAGVVPSVSTATDLAGSGQSYAGSAL
                     TGPQKLVDTLGAMQVDASYKLHGGLVSAIDAGLRYSSRIKSFTSLIGGTVNLTGTPSF
                     SSFNVAGGGFSTPSLIYANYDAISSLNLAGATSDPSQYWRVRESDVEGYLMAELAGAV
                     GAVPFSGNVGVRLVNVNTGSSAFQGLTTTAGTTYAPIYAPGHYFRALPSLNLNFDLTH
                     ELKLRAGVSRVMSRPPLDELRASQNLQYQPPSVLSGTAGNPNLKPFMATQGDLSLEYY
                     FHKDGVLALAGYYKDVDSNVGYTQYPVNIGGSTYAITGPANGKGGYIAGMEFALSTPF
                     WFVPGLQHFGLYTNVALVTSNLKEQAPSYNPFPAVGLADFTGEFDLWYSAHGVDARVA
                     VKHHSPYTVIFGWDATKLTRLASETTVGASISYALTKQVSLRFQANNLTNQAARFYYD
                     NDPNELARYEKYGRSYLADVTFKY"
     gene            complement(20653..21642)
                     /locus_tag="VFF98_04580"
     CDS             complement(20653..21642)
                     /locus_tag="VFF98_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU62937.1"
                     /translation="MVVRIKDVAAAAQVSPATVSRVLAGRAVDEAMRARVLAAVEATG
                     YRPNLAARRLRSRHTNTIGLIVADIRNPFFTAVSRAVEHLAYARGLRVILANTDEDPE
                     KEAMYLQLMQEERVTGVILAATRAGLDQLARQPFDFPTVLIDRTAPHVMADQVVLDNG
                     AMAGLLVDHLAASGHRRIAGLFGAASATGLERQLGYEAAMARAGLAAQAVPVPHGAGA
                     TERIVSALLTAPQPPEALIASNGVMLTAALRAVRALGAAAPPGLAMAGFDNEPWMDCL
                     STGLAVIEQPVEEIGRSAMAMLMDRLEHAEAPVRKVVLRGQLVLREAGQGTPG"
     gene            21801..24365
                     /gene="ptsP"
                     /locus_tag="VFF98_04585"
     CDS             21801..24365
                     /gene="ptsP"
                     /locus_tag="VFF98_04585"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZU62938.1"
                     /translation="MTLEALAIGVAPKDLIRIGAEVAGKEATIRQLGQMLVAAGCVSA
                     GYDESMVRREGLANTFLGAGIAIPHGLGEDKGLVRRDGIAVLQVPGGVEWNPGQVAHL
                     VVGIAANSDSHIAILRRLTRLIQDEARLAALFATSDAGEIAAALNEDGAPATAPAATD
                     YAEQRAWVLDYPTGLHARPATLWSEAAKAAGVPLRVRHGAESADPRSLVGLLQLGLKA
                     GDALTLSAQGPGAAEALDRFVALIASLSAREQAEAERAAAKRAAARPARGWQPAGDLA
                     FIPGVAASPGLAIGRIHVLAAAELDVPDRPVGLAEGGALLNEALVRTRAQMKAMVDDT
                     ARRLGPADAAIFAAQAALLEDTDLITLTCQLMVEGHGVAWAWHEAVQRMAGQLSALGN
                     PVLAARAADFRDVGRRVLEQIDPALGTGSLADLPDEPAILVAGDLSPSDTAALDVARV
                     AGLATALGGPTSHSAILARTLGLPSVVAAGAEVLGLAPGSLAILDGDAGRLWLAPDEA
                     DIASARAFLAAAAQRRAAEEAERGQPAITADGTRIDIAANVNRPDQVPFALAQGAEGV
                     GLMRTEFLFLERGHSPDEAEQAAIYAEMAAALEGRPLIVRALDIGGDKQVPHLDLPQE
                     DNPFLGVRGARLLLRRPDLLEPQLRALYRAAKAGGQFAIMFPMITSVSELLALRARCE
                     AIRAELAAPVLPIGIMIEVPAAAVLADVLAEHADFFSIGTNDLTQYALAIDRQNPELA
                     SEADSLHPAVLRLIAMTVAGAQKHNRWVGVCGGIAGDPFGAALLAGLGVSELSMTPRD
                     IPAVKARLRGADLAQLALLAARAQACAGPDAVRALDAQAQAQAQAQAQAPAAGEAA"
     gene            24365..25333
                     /gene="pfkB"
                     /locus_tag="VFF98_04590"
     CDS             24365..25333
                     /gene="pfkB"
                     /locus_tag="VFF98_04590"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HZU62939.1"
                     /translation="MRLLTLTLNPAIDLTISLDHLVPGEVHRARASRSDPGGKGINVA
                     SVLADWGLQPAAHGLLGRDNAAPFEALFAAKGIADKCLRVAGATRTNLKLVDAADTTD
                     INMDGPAITPEEAAAALAGVVAEAGPDALVVLAGSLPAACPPDTYAEAVAALRGRGAR
                     VLLDTSGAALQKALAAAVLPDVIKPNRRELADWAGRPLATMGDVLRVAGDLRARGVAL
                     VVVSMGAEGALFVGAGGALLARLAAGPLASTVGAGDAMVAGLAAALAEGADLPRMARL
                     ATAFAVAKLGRAGPHLPERACVEALADEVTIAAAETIGAGVRGEDE"
     gene            25330..27015
                     /locus_tag="VFF98_04595"
     CDS             25330..27015
                     /locus_tag="VFF98_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HZU62940.1"
                     /translation="MSRIWAVVNAAGAGVSGVLAAEALRKAARARGQDIEIELITEHG
                     AVNPITPAAVPAGAPVLWVGDAEDGHLLAARGAAVAIGEVLADPAGTLTAALAAAPAA
                     AQPMRIVAVTSCPTGIAHTFMAAEGLTEGARQLGHAIRVETQGSVGAGTPLSAEEIAA
                     ADIVIIAADREVDRSRFAGKRVFAGSTKPAIAGGARLITQALAEASLQAAAPGADAPA
                     PRADKPGPYKHLMTGVSFMLPFVVAGGLLIAMAFALGGIHANDEAAAGTLAHALFTIG
                     AKGGFALIVPALAGYIAFAIADRPGLAPGMIGGMLAANIGAGFLGGIAAGFIAGYAVA
                     GINRALPLPRNLAGLKPVLILPLLGSLVTGLAMLYAVGQPVAAVLAFLTEWLRGMQGS
                     SALLLGLILGAMMAFDLGGPVNKAAYAFSVGLISAQVYTPMAATMVAGMTPPLAVALA
                     TRLFANRFTPDEREAAGATAVLGLAFVSEGAIPFAARDPLRMIPSLMAGSALAGAISM
                     AAGVELKVPHGGIFVLPIPGAVSHVALYAAALVAGTVFSTLLIGLLKRPAAAA"
     gene            27078..28475
                     /locus_tag="VFF98_04600"
     CDS             27078..28475
                     /locus_tag="VFF98_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU62941.1"
                     /translation="MTVRHLAATLLAAAALAVPLRPALAEAAHAAAADSSADNADAAA
                     PDPQALPMPPAAQWQPLARQGISLSLSYTGEAAGNPTGGLRHASALTNQFYMGADADL
                     NTMLGWQGAAIHFAVTDRRGPSLSNTALGNNTSVQELWGTQNTHLAIATIEQKLWHGA
                     VDVEIGRSQANIHFLNSPIYCNFQSNSTCGNPTMVFKDSNLTYFPASSWMGHVKVTLA
                     PDWFAHAGVYEVNPERKAANDHGWSLSTAGATGVVVPWEAGYAHEGGDGRAAAHYILG
                     GWFDRADYADPLRDGNGGIAVLTGQAPATRHGRSGLYFRFDRQITRPVAGSQRGLSLF
                     GIVMANTSGQVEESSFWELGLLQTGTFKHREADTLGFMINDQRFSDLAMQRMAAARAL
                     AGGSDPVHRHEYMMELTYGLQVASAIRVSPNIQYILHPDNTGAPYRTTNTPDTLVLGL
                     KFTIDALGLARMGLR"
     gene            28664..30946
                     /locus_tag="VFF98_04605"
     CDS             28664..30946
                     /locus_tag="VFF98_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HZU62942.1"
                     /translation="MRFISKIRVAALAVPAGTLGLPALALADCSTSGTTVTCTGTTSG
                     FSTTTADTAITVNSGATVVGPLAASGDGSTLTNAGTIYESSAVYAVQMGDDATVTNTG
                     TISSTSSSSGAGAISVGADSTVYNHGSLTAESGTYAVSFGSNGTFYQESDATAELTGN
                     INFGTNTGSNIASLYNYDTTYGIYGNVYASGNVQVYNDGIFEGNFYQTAVAGSSTVTF
                     DNGSAATYVGIIQTGNNTTLTNEGTMYLYSGSVIGSYYSSGTDGTSSVTNSGTLYVGT
                     VSAPAALDIYGDFTQTSAGTLNMAISGTGSAGTTYSQIYTSGTATLSGTLNINTSSGY
                     VADSTVYKLIDAVGGISGNFTSVTYNGNSSSLFLDWDIIGAVSSGSGSEQYYEVSATH
                     TGYANAMRSNGGTNNQIAVASGLDPLISIANADTTSGEATLLGEVDALNLAQAKVFLN
                     SVSPEGYLAYATALRDQANMFARTVDQRMTDQNSKHPEDGWWFTTMAQFDFASTASTQ
                     GGYRTRDHMFGLAAGYDLSGPNHVIGLATSISWDSLHYALNSLNGTNRDWAVALYGAQ
                     NFGRLRLYGQIAYNMGHLSTTKTITIGDYTWTANARAPEHLFKATANLGYTWSLGQYK
                     LEPFGGIDFNSGRVAGFTEYNAGAADLTVSAMSASHTDVLAGVNVTRNKGIFRPYFKM
                     AYRRQLTGDRNTVSAYFDGQSSGTFSVTGLPVGRDEIDPSAGINWVFDDAGSLYVGYQ
                     GTIRSNYNSEGINLGIRLEF"
     gene            complement(30986..32101)
                     /locus_tag="VFF98_04610"
     CDS             complement(30986..32101)
                     /locus_tag="VFF98_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZU62943.1"
                     /translation="MPSSDILFRPLRIGTLELANRIVMAPMTRSFAPQGVPGAANAAY
                     YRRRAEGGVGLILSEGTVVNRPAARNDPGIPFFHGEAALAGWQGVIDEVHAAGGKMGP
                     QLWHTGATKSFQTEWVPEAPVESPSGLNAPGDPRGVAMSEADIADTIAAFAAAAADAR
                     RLGFDTVELHGAHGYLIDEFFWAGTNLRTDRYGGTTIAERARFAAEVVAATRAAVGPD
                     FPIIIRLSQWKQQDYAARLATSPEELGQWLTPLVEAGADVLHCSQRRFWEPEFPEIDG
                     ANGLNFAGWAKKLTGATTISVGSVGLSGEFIAAFGGEASTSVGLGQLVERMERDEFDL
                     IAVGRALISDAQWAAKVRAGDATGLKGFDASALAELV"
     gene            32201..32566
                     /locus_tag="VFF98_04615"
     CDS             32201..32566
                     /locus_tag="VFF98_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU62944.1"
                     /translation="MHVCQHFSVDCPSRQLFDQIADKWSMMVLTVLDAGPMRFNAIRR
                     HLEGVTQKALTQCLRRLERNGLISRRVIARSPVAVEYAITALGCSLHRPFKALYGWTL
                     ANMAEVEAARRAFDARQAA"
     gene            complement(32571..34295)
                     /locus_tag="VFF98_04620"
     CDS             complement(32571..34295)
                     /locus_tag="VFF98_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZU62945.1"
                     /translation="MRHLSLPARLLRPVLHLARRLSADNRGNVLMIMGFAIVPMIFAV
                     GFGIDYSRAMKLQTQINAAADAATLSVVSATAMQQSQGSLSNAQFQAQLIAQMEANFA
                     AQTGNLTGLSGSIAYGSGTTVTLTGTLNNGRTAVLNWTAQSKNVFAGILGASTLTISG
                     TSSAGATGAPYINFYLMLDNSPSMLLPSTSAGLSAIATATSATTNAPYGCEFACHEQN
                     PNGGKPYVYNGSTSIFLLSNYYSSGTTTNYYKVNTSTGAVYNSSGSQLSGYLYCSSSG
                     GVYQATRCGSGWGGTQASSSNQVAGYWADGYWLTHNYTILFPSGSTVNLRIDDERSAA
                     QALIPFAQTTAKNNSVAYQMQVFKFDWTHTYNSQTTPVTALTSAMADVKNMTASQIPD
                     LWAAQDFWYSNNWPTSSYSNGDQGSEFLNMLTYMNNLMPAPGDGSTASSPQQVLFLVT
                     DGMSDEAVSGSRTVSPLNASDLAVCSTIKSRGIKIAILYTQYLPDSLDGDSWSQSVVA
                     KYLPAPSNYTSTTAGTTDQVATALQSCASTGGNGSALFYQVATDQSISTALNALFALT
                     VQSAHLVQ"
     gene            complement(34316..34978)
                     /locus_tag="VFF98_04625"
     CDS             complement(34316..34978)
                     /locus_tag="VFF98_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62946.1"
                     /translation="MAARLPWRWPIRLPAALARLAHSRRAVAVVEFAIALPVLIALYM
                     LAFVMSDMIACSRKVTITARALTDLATRSAYLSTNSSNAAYIGTLMAAAQQVLAPYAA
                     SNAVITLSQVQVVDSTHVIVVWSQSYSGSTLSSGYTAGTSITTTSGLIPSLLLPSGST
                     KQSICTTATSLTGDGGCLIYGEVRYAYTPSFWYGSASTITFYQPIIMSPRLSASIALV
                     TS"
     gene            complement(34978..35547)
                     /locus_tag="VFF98_04630"
     CDS             complement(34978..35547)
                     /locus_tag="VFF98_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZU62947.1"
                     /translation="MRDSRGNAALEFAFVAPAFIALVVAIFQIAFIFLAQQQLETATE
                     GAARYILTGQAQNGAWSAATFRQKTCALLPAFMSCSNLYVDINTVTSYAQASTSQPLM
                     SASTYTVTTSSAAGATCTTSSVAAGSADANGITAAFNYCTGTQGAIVQLRLLYIWPTL
                     SAPLGFTLANGGTNQRYLLSTSVLKTEGY"
     gene            35836..36408
                     /locus_tag="VFF98_04635"
     CDS             35836..36408
                     /locus_tag="VFF98_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZU62948.1"
                     /translation="MPPAELLLIRHAPVLADGRLAGRRDVAAVLPDAAVLAAVRALAG
                     PVDRVIASPARRCRATAAALWPEAALVEDARLWEQDFGAWEGLPFADLPDLGDLAPAA
                     LAGHAPPGGESFADLCARTAPALEAWAALPGRSALVAHAGTVRAALALALGAAAPALA
                     FRIDPLSLTRLQPCGGQGWAVGGVNLVAAC"
     gene            36402..37382
                     /gene="cbiB"
                     /locus_tag="VFF98_04640"
     CDS             36402..37382
                     /gene="cbiB"
                     /locus_tag="VFF98_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HZU62949.1"
                     /translation="MLSALAFHAAALLVALALDALLGWPAWLYARIGHPVSWLGRLIA
                     AADCACNREGAAFALRRAAGVAVALGVIGLAAGAGWGLAGLVAGLVPAGWPGVVLLGA
                     LCWPLLAAGSLHAHVAAVARPLAAGDMAGARQAVAMIVGRDPDRLDAAGIARAAAESL
                     AENTSDGVIAPLFWGLLFGLPGIAGYKAVNTLDSMIAYRTTRHRAFGWAAARIDDAAN
                     LLPARLTGLFYALASGRPWAALRCMAADARHHRSPNAGWPEAAMAGALGIRLSGPRLY
                     AEGMSQDRWLNGAAPDPVAADLAAALALFGRVVGLAALGLALLAVGVGGI"
     gene            37382..38380
                     /gene="cobD"
                     /locus_tag="VFF98_04645"
     CDS             37382..38380
                     /gene="cobD"
                     /locus_tag="VFF98_04645"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HZU62950.1"
                     /translation="MRDHGGNLDAARARFGGEGWIDLSTGINRVPYPLPPLPAEAWTA
                     LPTASARAALLQAAAAAYRTTAPMVALAGAQAAIQLLPQVVQGRHARVLAPTYNEHAA
                     CLAAAGWQVAQVADAADLAGADIAVVVNPNNPDGRALPPEALLALAGQVGLLVVDESF
                     ADPVPDLSVAPWAGAGVDQGRLLVLRSFGKFYGLAGVRLGFALGDGAVVAALDALSGP
                     WPVSGPALAVGAQALADGAWAAATSARLAAEGPRIDALAAGFGWQLAGGTALFRLYDT
                     DDAEAAQAHLARQRIWSRIFPWSPRLIRLGLPGDAAEWQRLAEALAPPTPASRASG"
     gene            complement(38352..38879)
                     /locus_tag="VFF98_04650"
     CDS             complement(38352..38879)
                     /locus_tag="VFF98_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62951.1"
                     /translation="MKDTFDLEAEVRDLAARRDIGRALMRYMRGQDRLDAALHRSAFH
                     PDAWVDCGLMAGSAAEFVDFAQDLLAGMEGTQHIIAQTDIDVRGDAASGEIYFYAWHR
                     LLQEGEAQDLMVAGRYVDEYACRDGEWRILKRREIIDWARTDAASDGFLAEHPYLPRG
                     QRCGADFSQTRDWPV"
     gene            complement(38888..40300)
                     /locus_tag="VFF98_04655"
     CDS             complement(38888..40300)
                     /locus_tag="VFF98_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU62952.1"
                     /translation="MLDAIRAHGLPDAQGEANLASARCPAPSVQEIVAKDALPPPPVL
                     AHEEPAYLGSAPVSVDRYYDQAIFAAEIEKIWKKSWQWVCREDHIPEPGDYYTYEVAH
                     LSYLVVRQEDGSVKGFVNSCLHRSTKFKRTEGFGSAGEIRCPYHGWTWHNDGALKAVP
                     CAWDFGHVCADKTALPEVRVGHWGGFIFINPALEGPSLAEFLAPLPEHFRDWDIDKRF
                     VELHVAKELPCNWKTAQEAFLESYHVLETHPQLMKGVGDANVQYDIHSDHVTRFYAAS
                     GINSPHLAQPLSEQELVSTMILGDRSVLDPGLAVAEGESARTVMARFLRQVMGEKYGC
                     DLSRFSDSEMIDTIEYHVFPNMVLFPGISLPMAYRFRPIGLDPNRTLFEILFLRPSPD
                     QGAAPEPAEPYRVREEESYASAPGFDPGMGAVYDQDTDNLRAQQEGFRASATGVQQLG
                     NYQEVRIRHFQQVVDKYLSA"
     gene            complement(40404..>40982)
                     /locus_tag="VFF98_04660"
     CDS             complement(40404..>40982)
                     /locus_tag="VFF98_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62953.1"
                     /translation="ESPAAGEAPPLALLLDNPAEIPADAALNFTLKASGEERFTGREV
                     VEVSTADGTASTRLTLGSGLTLVDPAAMVASLTPARALGASAFGTLRARLVRAGVAGD
                     WLNLGTLVRLPRLKRLSCPATGPCTLAGEGLYLLASLSATADFDKATSVAEGYPGFSL
                     PVPRPGTDGVLYVRLHDAPEVVNRLRAAPAGR"
BASE COUNT         6358 a        13482 c        14508 g         6634 t
ORIGIN      
        1 tggccaaggg tttctggaag aagcggccga aggatattgc cggcatcggc ctggtttacg
       61 ctcgggtgaa cccgcgcacc accgaccgcg agcgcgagct gctggccgcc ggcaaatccg
      121 ccaccgtgca gacctatgag gccagcggcg aggtctatta cggcttccag ttcggcccgc
      181 tcatggtgcg gcccaatgtg caatatatca accatgtggg cggcagcagc cgctattccg
      241 acgcgatcgt cggcggctgc acgatccgcc tgacggtgtg aggcaagagg gggcgggggg
      301 cgggggcgtt aacccccgct tcctctcaat ccaccggttc tgccgggcgg aagcgggttt
      361 gcgcagccag gcgcacgccg gcattggggg cgccaaggcc ggtgtagcca tcgccgcgtt
      421 cgacgaattc gaagaagaag cgccggtcga aggcgcggct gtaaagctgg gtgtagcggt
      481 ggccggcggc atcggcatcg aacagcaggt ggccggcctg catggcctcg gtctcggcct
      541 ggctgaagcc gaagcgggcg gccagatcgt cgtaatagtt cgccgggatc tccagcaggc
      601 aggcgctgcg ggcggcaagg gcggcgctgg tggcggcggc atcggcggtg gcaaaggcga
      661 tgtgctggaa gccgccggtc atcccctgcg cgaggaagcg ggccgaaagc gtctcgcgcg
      721 cgtccgatcc gttgatggtg acgcgaaagg cgccatcggc cgtttgcagc gcctggctgt
      781 ggacgaggcc cgaagggtcg atcacatcct gctcgctcag cgcttccagc ccgaacaggg
      841 cgcgccaata gagctgccac gacaggaatt cgtcattgtg caccaccgag gcgaggtgat
      901 cgatgcctga aagcgccggg gccgcggtgt cgatcggctg gtgggggccg tcaaattcgc
      961 gggcccacag gctttcgctg gcatcctcgc cgatgagata gaccaggctg ccgcccaccc
     1021 cgcgcagcgc cggcatggcc agccgcccgg gccgcccttc cggcgtgaag cggcggatgc
     1081 ccagcgccgc cgcgcgcgcc agcaccgctt ccacatcggc cacccgcacg ccgatcgcgc
     1141 agatcgaggt gccgtagacg gtgcgatagg tgttggcaaa gccgcgcggc tcggcgttga
     1201 tgacgaggtt taccgcgccc tggcgccagc gcgtcacctt cttggtgcgg tggctgcctt
     1261 cctgggcaaa gcccaggctg gcgaaagtgt cggccagcct ttgtgcttcc tcgccggcgg
     1321 cggtgaattc gacgaattcg gtgccggtga tcttttgcgc tgcgggcatg gtggtgggct
     1381 ggcccagcag gcgcctggcc gcatcgcgca catattccag cgagcgatag ccatcctcgg
     1441 ccaccatccg cggcgaatgg gagcggaacc ggtcgttgaa gatttccagc gagagcgggc
     1501 catcatagcc ggtgcgcagg atctcggcca cataggcggg cagatcgaag ccgccctggc
     1561 cgggcaggtt gcggaaatgg cggctccagt agagcaggtc catgtcgagc agcggcgcat
     1621 cggccagctg cacgaaggtg atcttgtggc cgggaatggc gcggatgctg tcgctgggga
     1681 tgcggcgcga gagcgagtgg tagctgtcga ggatgatgcc gacatggggg tggtccaccg
     1741 ccttgacgat ctcccacaca tcgcggtgat cgtagatgtg gcggccccag gccagcgctt
     1801 catagccgac atggatggcg cggcgcccgg cgcgttcgcc cagttcggcg aaatcgtcga
     1861 tgatgcgctg gcgctcgccc agcgactggg gcgagcagtt ggagcagacg aggatgcgat
     1921 cggtgcccag ctctcccatc aggtcgaact tgcgctcggc ccggtcaaac gcgcgctggc
     1981 gcagggcgcc gggcatcccc tcgaaatcgc ggaacggctg gtagagcacg caggccaggc
     2041 ccagatctgc catcatccgg cggatttcgg ccgggcgctg gggcgcgccg atcagatcgt
     2101 tctcgaagat ctcgaccgcg cggaagccgg cgctggcggc cgcctccagc ttctcctgca
     2161 aggtgccctt cagcgagacg gtggcgatgg cgttgatcat ggctgggctc tcccgtttgg
     2221 ctggcgctgg tggccggggc ggattttagg ccagcggggc ggcgggaaaa gaggggtaga
     2281 ccggctttcc cataccattg tgttatgggt gagggggtaa ggttatttac ttatcttttg
     2341 gccgggatat gctttgtgat tgggtgaata gatatagaat tatctttttt ctctgcaaat
     2401 aattttttgc aatcgttatt tatacatcga cgcattattc ttttaagcgg tgaattgcgg
     2461 taagctttct ggcgcgcaga ggagatggct ggcgcgcaag ggggtggccg atgggccaat
     2521 ctgttcccgc agcggttgtg tggggagctg catgggcgct ggcaacgggg cccgggctgg
     2581 caggcggggc ggcggcagcg ccggcgggcg atgtgctggc gccggaggag gcgcgcgtca
     2641 tcctcgttac cgccgctgcc gccggcaaaa ccgcgcagga cagcgccctt gccgtctccc
     2701 aggtcgatcc gcaggcgatc gcccgcttca ccccccgctc gcaggccgag gcgctgcgcc
     2761 ttatccccgg cctcaacctg caggatacgg ccgggcccgg cggcaatgcc aatatcggcg
     2821 tgcggggcat tccggtcagc actggcgggg cggaatatgt ggcgctgcag gaagatggcc
     2881 tgccggtggt gctgtttggc gacatgctgt tcggcaacaa tgactattgg ctgcggctgg
     2941 acaataatgt ggcgcgggtg gaagcggtgc gcggcggctc tgcctccacc tttgccagcc
     3001 aggcgcccgg cgcggtgatc aactatgtgt cgaacacggg ggagcgggcc gggggcgggt
     3061 tttcgctctc caaggcgctg gattatcgcg aaacccggat cgatgccgat tatggcgggc
     3121 cgctgggcga tcatctgcgc ttccacatcg gcggctatgc gatcaccggc ggcgggccgc
     3181 tgcattatgg ctttgccgcc gagcatggct atcagatcaa ggccaatctc acccgcagct
     3241 ttgcagatgg cggcggctat gtgcggatca gcctgaagcg gctggacgac cgcgagccga
     3301 ccgaccagaa catgccgacc atcgccacgc tggcgggcaa cacgattacc ggctttgcca
     3361 atttgcccgg catcgatgcg cgccagttca ccccgatcgg cctttataac gccagctttc
     3421 ccatgctgaa tggcagcggg cagctggcca atgtgccggc gcagggcatc cacccggtgg
     3481 ccaccgcgct gggcgccgag gtgcagcggc ggctgggcgc ccatctcacc ttcaccaacc
     3541 gcttccgctg gacggcgatg aagggccagt ttgccttctt cttcagcggc gtgcgggcca
     3601 cgtccactgt ggcggccaat ggcgccagcc tgcgctatgc ggcggggcca gcgctggggg
     3661 gcacttatgc cggggcgctg gtcaatgccg gcacttatgt gaacaccagt atggacgaca
     3721 tgggcagcct ggccaatgat ctggtgctgg gcggccaggc gtcgctgggc aggctgggcc
     3781 tggcggtgca tctgggctgg ttccacatgc gccagaagat cgctgcccac tgggccgtgg
     3841 ccaatggcta tctcagcgcc accagcagcg ccaatccggt gccgctggat ctgtttgccg
     3901 ccgatggcac gcaactggcg gccaatggca ttgccgatta tgtcgatcac tggggcgaca
     3961 atggcgcggc cagccagcgc cactatcgcc tcgcctatac cgacgatgcg ccctatggcg
     4021 agctggcggc cgatatcggc cggctgagcc tggatgccag cctgcgcgtc gatctgttgc
     4081 gggcgacagg ggcggtgtat caatctgccc gggccagtgg cacgctggcg gtcagcgatg
     4141 cgctgggcac ggcgctgctg ccctatgcgc tgccttccgc cacgccgggc acgctcctcg
     4201 cctatgggcg ttcctatgcc agctggtcgg ccggcgggct ctatcggctg ggggcgggca
     4261 ccaacctgtt tatgcgggcc agccggggcg ggcgcttcaa tgccgatcgg gtggcggtca
     4321 atccgctcaa tttcacagcc gccggcgggc tgtcggccgg gggggcggcg gtggcggtga
     4381 attatgtggc ccagcaggaa gtggggctga agcgcgcagg cggagcgggc gcctttgcct
     4441 atcatctgga agccaccttc ttccgcgccc aggtggtgga gcataattac gatttcaccg
     4501 cccccacgcg cggcctcagc ccgtttgtcg atgcgctgta tcatgcccat gggttggaac
     4561 tgtcgggcga ggtgcggcgg gggggcttcg tgctggcggg ctatgtcgat tatacggtgg
     4621 cgcgcgatct ggcgcgcaac ctgcccgcct atgcgctgcc gcgctggaat gtgctgctgg
     4681 cgccggccta tgatgcgggg gtggcggccg ccggcttcag cctctcgggc cagtctgcct
     4741 tctatatcgc caaccagcgg gtgacagcgc cgggcacctt cttcgtcaac gcttttgtgg
     4801 cgctgcgccc gcgcgcgcgg gtggagatcg ggctgaacgc caacaacctg ttcaacgcgc
     4861 tgggctaccg ggccaacaat gccgggctgg gcccgccggg tgaacagggc gtgctggcgg
     4921 gcaaccaggc gatcttcaac aattccatcg tggccgggcg cagccttaca gcggcgctgc
     4981 gctatcgctt ctgagccggc gtggcgaggg ctgcggccag catcagcatg ccggccagcg
     5041 cgatggcccg cgccggatcg ccccctagca ccacgcgata gacccagccg aagctgagcg
     5101 cgtgcagcag catgggcaca acgatcatcc cattgtaaag gcccatgtaa acgccgatct
     5161 gctggcgcgg cacggcgcgg gccaccagca atggcggcag ccccatcatg ctagcccagc
     5221 ccagccccag ccccagcgcc gggcccagca gcagcaagtg gcggcccagt tcggcaaggg
     5281 caagggcgct gaagagcggc ggcgccgcgt tgctgccggc gcccgccagc ggcagggcca
     5341 gcaagccaag gcccatggcc accatcgccg cccggtgcag ccgccccgcc cccagccggc
     5401 gtgccagcgg caccatggca aagcccgccg cgcaggcgac aagattatac accgcgccca
     5461 gctgctggct ggccagcacc gcggcgcgaa aggccgcgct ggtcttgtcg gccgtggcat
     5521 aggcggtgcg gccgatcgcc agcgccacat aggaccagta gatcatcgcg ccataccact
     5581 ggaacaggct catcgccgcc aactggcgca tgggggcggg catggcgcac agcgccctca
     5641 agatggtgcg cagcgcgttc cagatggcgc ccagcggcgc gccggctggc aggcggtcgg
     5701 ccgggggcag cggctcctct ggcacgcgcc acaccgacca cgtgaccgtc gccagcgaca
     5761 gcaccgcgcc caccaggaag ctggcgcggg tgatggcggg caggtgatgg gcgctggcgc
     5821 tgccgctcac ccccagctga tagagcagca gcgagggggt gagataggcc aggctctgcg
     5881 ccagcccggt aaaggcggcc tgcacgagaa agccggtggg ctgcgcgcct gcatccagcc
     5941 ggtcggcgat ataggcgcga tagggctcca tcgtggtgtt ggcgccggca tcgatcagcc
     6001 acagcaggct ggcggccatc agcaggctgg tggaaaaggg catcagcgcc agccccaggc
     6061 tggtgagcac cgcgcccgcc agcacatagg gcaggcgccg ccccagccgc cagcaggtgg
     6121 catcggaaag cgcgcccacc agcggctgca cgagaagccc ggtgacgggc cctgccagct
     6181 gcagcatcgg cagccgcgcc tcgcccgccc ccagccacga gaggatcggc gccatctgcc
     6241 cctgctgcag cccgaaagcg aattgcaggc cgaaaaagcc gaggttcatt tccaggatgc
     6301 gcagcagcga caggcggcgc ttgtgcgcgg ggcctgtcgc tgcggggagg ggctgggcca
     6361 tgggcatcct ttgcggcggg cagtgccacc gggcagcgcc gctgcccagc atggcccgcc
     6421 ttggccgcca agggaaagca aagaatgcgc cgctgtgggc gcagcttatt ccgctttgcc
     6481 gagcgctgcg acaaaggcgg ccgcccgcgc gcccacttcg ccggccgaaa gccccggcgc
     6541 atagagcgcc gagcccagcc ccgcgccatt ggccccggcg gccatccagg cgcccagatt
     6601 gtctggcgtg acgccgccca ccgccagcac cggcaggccc ttgggcagca ccgcgcgctg
     6661 ggctttgaga taggccgggc tggccccttc ggcggggaac agcttcagcc cgtgggcgcc
     6721 ggcggcaagg gcggcaaagc cttcggtggg cgtgaagaag cccggcagcg agaccagccc
     6781 ggccgccacg ctggcgcgga taacggccgg attgctgtcg ggcgagacga tcagctgccc
     6841 gccggccgcg gccacggcag ccacatcccc ggtggtgaga acggtgcccg cgcccaccac
     6901 cgcggccggc cccaggcgcc gcgccagccg gctgatcgat tccagcggat cgggcgaatt
     6961 gagcggcact tcgataaggg tgaagccggc ttccaccagc gcctcgccga tcgcttccac
     7021 ctcatcgggg cgcaccccgc gcaggatggc caccagcggg cagcgggcaa gggcggtgtt
     7081 caatgcgtgt tgcggcgtgg tcatgcttgg ctcgctttcc agatgcgggt gatgccggcg
     7141 atgaaaccgg cgtggctgtc gagcaggaca ggctgcccgg cggccaggtg aacggcgctg
     7201 gcataaagcg cgcccagctg cggatcggcg aggatatgca ccggatcgcc ctcgcgcagg
     7261 gcagcgccgg ccacatcatg gccgatcagc aggcccgatg tgtaggccgc gctgtcttcc
     7321 accttgcgca ggcccagcag ggcgctgccg cgcacgcgaa agaggtggct ggtcaaccgc
     7381 ccggccaggc ccgcccgcac gccggcgcga aacgcctcgc catcggccac ttcgccggtg
     7441 aggaattcgg ccagcaggct gtgggccttc agcagggcga acagctcgcc ggtcatcgcc
     7501 gtggcaaagc cggccacgcg gccttctgcc atgcgcgccc atttgcaatg ggtgcccggc
     7561 tggcacagca gcgcattatc gggcgccagg ccggcggcaa cggcgcccag cagctgcact
     7621 tcctcgccgc gcatcacatc gccggccgga tcggccaggc ccggcacgat ggccgtgcgc
     7681 cccgcttcca cccagtgaag ggcgccggcc agatcggcca agccggccgg gcacggcaga
     7741 tagggcacat tcacccaccc ccgcgccgag cccaccatgc ccgccagcag caccggcaga
     7801 tcgccaaggt ctacccggat ggcggccact tcatcggcaa agccgccggc cggcacggaa
     7861 agcaggccca tcgcatcgcg ccgggtgtgg agcaccgtgc catcgccatc cagcagatag
     7921 gcgcggcggt tggtggtgcc ccagtcgatg gcaatcagct gtccgttcat ttcaccacca
     7981 caccgcataa agcaccacca gaatggccac caccagcgcg ctggcaaagg caaagccgcg
     8041 gctggtgccg aaatggacac cggccatgat gcccggccct tcacgcgccg gcgccgggcg
     8101 ggccagcgag acgaccaccg tgagcgccag cgaggcggca aagacgatcc ccatgcggtt
     8161 catgaaggga acagcggcaa gcgccgcgat gccgccaaag cggaacagcg ccgaaagcac
     8221 cacgctggcc acagcgccgg ccagcgcgcc cgcttctgtg gcgcggggcc agaacaggcc
     8281 ggtgaggaag atcaccgtga tgccgggcgt gacgaagccg gaaaactcct gaatatactg
     8341 gaacgcctga tccagcccgc ccagcagcgg gcgcgcggcc agaatgccaa gcgcggtggc
     8401 cacgcaggca gcaatgcggc ccacccgcac cagctgcgcc tcgctggcgg ccggcgcgat
     8461 cttgcggcga tagagatcga gcgtgaagat ggtggcgatg gaattgatct tgctggcggt
     8521 agacgcgatg atcgccgctg tcagcgcggc aaacaccagc cccagaaggc cgctgggcag
     8581 caggtgcatc attgccggat aggcctgatc gggcttggca aggttggggg tcagcagcac
     8641 cgcggcaatg cccggcagca cgatgatcat cggcatcagc agcttgagga aagcggcaaa
     8701 gacaatgccc cgctgcgcct cggccagcga tttggcagcc agcgtgcgct ggatgatata
     8761 ctggttgaag ccccaatagg agaggttggc gatccacatg ccgccgatca gcacggaaag
     8821 gccgggcagg tccttgtagt gcggattgcc gggcgagagg atcatgtgga aatgatcggg
     8881 cacgcgggcc accagcgtgg caaagccggc ggcgatgccg gcccccgcgc ccagctggcc
     8941 cagcgccatg cccgagacca ccagcccgcc gatcaccagc agcgttacct gcacgatatc
     9001 ggtgagcgcc accgccttga ggccgccgcg gatctgatag gccagggcaa acaggcccag
     9061 gcccgccagc gccacatcct ggttgacacc ggccacctgg gtgaccgcga cagagcccag
     9121 ccacaggatc gaggtgaggt tgacgaagat gtagagcgcc agccagaaca gtgccatcac
     9181 tgtgcggata gtggggccaa agcgcgcttc cagaaattcc ggcatggtgg tgatgcggtt
     9241 cttcaggaac accggcagga agaacttgcc aacgatcagc agggtggccg cggccatcca
     9301 ctcgtaagag gagatggcaa ggccgatggc atagcccgat cctgccgtgc cgacgatctg
     9361 ttcggccgaa atattggcgg cgatcagcga ggcgccgatc gcccaccacg gcagcgcgcg
     9421 gccggccagg aaatagccgg cgctgttttc cgcctcgccg ggtttgcttc tgctcaccca
     9481 ttgcgccagg gcaaagatga agcacccata ggccaccacc atggccgtat cgatggcaga
     9541 cagggtcatt cgtatgcctt tcctctcgtg ccccttgcca aacggggggc tggtctattt
     9601 atatgattaa acggaatctc gaagggaaca ggccaatgga cagtgatgca gacgacggcc
     9661 gcggcggcgg gctgggccgc aatctgacct atggcctgct cgaccatctg ggccgccaga
     9721 tcgtgaccgg cgcctttgaa accacgccct ttcccaccga ggcggaactg tcggagcagc
     9781 atggcgtcag ccgctctgtc acgcgtgagg cggtgaagat gctgaccgcc aagggcctgc
     9841 tgaccgcccg cccgcgccag ggcaccagcg tgcagccggt ggccagctgg aacctgttcg
     9901 atgccgatgt gctgcgctgg ctgctggagc gcaagttctc ggtcgatctg ctggcgcagt
     9961 tcaaccagct gcgcgcggcg atcgagccgg ccgcggccgc ccttgccgca gaagcggccg
    10021 atgcgcgcgc gctggcgctg atcgaggccg gctttgcccg catggaagcg gccgatcgcg
    10081 gcgaggacga cgggctggat gccgatatcg cctttcatct cgccatcctc cgggccagcg
    10141 gcaatccctt tttcatgcag ttccgcgaac tggtggcaac ggcgctgcgc acctcgatcc
    10201 gcttcaccaa ccgcatcgtt ggccgcacgg cagatctggc cgcccatggc gcggtgaagg
    10261 aggcgatctg cgcgcgcgat ccggcgcggg cgcagcaggc gatggcggcg atcatcggcg
    10321 atgtgatgag cctgatcgcc gagcagcgcg ccggttgatt ggggccggct gatccggaag
    10381 aaggccgcat cgctcagcct ccgaagcggt ggggggcaag gccggccacg cccgcctcca
    10441 cctcgaacac gctgccggcc agcggctcgt ccaccccgtc ggccgcgctg gtgaggaaca
    10501 ggcgggtgta gccggggccg gcaaagcagg gcttggtgat ctggctggcg ggcaggtcta
    10561 tccagcgttc acgcgcgcct tcgggtgtga agcggctgac gcagccggcg ccccaatggg
    10621 cgatccacag gcccccctcg gcatccaccg tcatgccatc gggcgaaccc cattcgggcg
    10681 gaaaggtgag gaacagctgg cgcgggccca gcgtgccatc ctcctgcagg gcaaagcgat
    10741 agacgaggcc tttggcgctg tcggtgtgat agaggagggt gccacagggc gagagggccg
    10801 ggccattggc aatggcatag ggcgcatcca tccgctgcca ggccagatcc ggatccagcc
    10861 ggtagagcgc gccttcctcg ccgctgccat cggcgcgcat ggtgccggcc cagatgcggc
    10921 cttgcgcatc cacgcaggca tcgttcatcc ggttggccgg cagatgcgcc tcgggcgcgg
    10981 ccagcggcgt gagcgaaaag ggcgccaggc tgagcgcatg gaagcccgat tgcagcccgg
    11041 cgataaagcc ctggccattg gcgcgttcca gcagccagcc ggtcatctcg ggcatctgcc
    11101 attcggcata ggcgttatcc gccaggccaa agcggtgcac ccgctggccc aggatatcca
    11161 cccagtagag cgcgttttgc gccgccacac aggtgatccc ctcgcccagc cggtcacggc
    11221 gcgggccgcc cggggcgggg cggggcagga tggtccattc ggccatggct ttatcccttt
    11281 agtggctgtc gcgcggcagg cccttggtct gggcgatgcg ctgatatttc acggcgcttt
    11341 cgatcaccgc gccgccggcg aattgcccgg tgatctggcg ggaaatctcg tgccaggggg
    11401 tctggtggtc ggggccggtg aagggggcgg cggcttcctc ggcgcggcgg cgggccatct
    11461 cctcctcgct gacgagcagg ttgacctgcc gcgccctgag gtcgatgcgc accacatcgc
    11521 ccgtgcgcag cagggccagc ccgccgccgg ccgctgcctc ggggctggcg ttgaggatgg
    11581 acgggctgcc gctggtgccc gactggcgcc cgtcgccaat gcagggcagg gcatcgatgc
    11641 cggcgcggat cagcgcggcc ggcgggcgca tgttgaccac ctcggccccg cccggatagc
    11701 cgatgggccc ggcgccgcgg atcaccagga tagagcgacc ggtgatgcca agggcgggat
    11761 catcgatgcg ggcgtggtaa tcctcggggc catcaaacac cacgacatgg ccctcgaagg
    11821 cttccggatc ctgcggatcg gccagatagc gggcgtggaa ggctgcggag atcacgctct
    11881 tcttcatcac cgccgcctcg aacagattgc ccgacagcac ggtgaggccg gccgccggct
    11941 tcagcggggc agacagcggg cggatcaccg cctcatcctc gatggtggcg ctgccgacat
    12001 tctcggccag cgtgcggcca ttggcgttca gggcgctgcc atccagcagg ccggcgcgat
    12061 aaagctcgcc catcaccgcc ggcacgccgc cggcgcggta atagtcctcg cccagatatt
    12121 cgcccgccgg ttgcaggttc accagcagcg gaatgtcggc cccctgttcc tcccaatcgg
    12181 ccagcgacag ttccaccccc acatggcggg cgatggcgtt caggtggatg ggcgcgttgg
    12241 tgctgccgcc gatcgccgaa ttgacacgga tggcattgag gaaggccgcg cgggtcagga
    12301 tatcggaagg cttgcgatcg gcgcgcacca tctcgacgat ctggcggccg gtgtgccagg
    12361 cgctttcggc ccggtcgcga tagggggcgg ggatggcggc attgcccggc aggctcatgc
    12421 ccagcgcttc ggccaggctg ttcatcgtgg tggcggtgcc catcgtgttg caatagccgg
    12481 tgctgggggc cgaggaggcg accagctgga caaagccctt gtagtcgatc tcgccggcgg
    12541 ccagcatctg ccgggccttc cacacgatgg tgccgctgcc ggtgcgctcg cccttgtggt
    12601 ggccgttcag catggggccc accgaaaggg cgatggccgg gatgttcacc gtggccgctg
    12661 ccatcaggca ggccggcgtc gtcttgtcgc agccgatggt cagcaccacg ccatcgatcg
    12721 gatagccata gagtgtttcc accagcccca gataagccag gttgcgatca agcccggcgg
    12781 tgggccgctt gccggtttcc tggatcgggt gcagcggaaa ttcgatggga atgccgccgg
    12841 cctcgcggat gccatccttc acccgctggg ccagcgtcag atggtggcgg ttgcagggcg
    12901 agaggtcgga gccggtctgg gcgatgccga tgatcggccg gttcgactgc agctcctcga
    12961 tcgacaggcc atagttgaga tagcgttcga gatagagcgc ggtcatgtcg ggcgaggcag
    13021 ggttgtcgaa ccacgcgcgc gagcgaagct gcggcttgtg ggggctctcg gacatgaact
    13081 caggctcctt cttgcccatt gtggcaggcc gggccgactc cggttctggc ggtatcggct
    13141 tgcggttgac ctctataagc atatatatca tataaaagta aagcggagga tgtcaggcat
    13201 gggtgtgggc atgggtaatg gtgtgggtgg cgggggtatg ggtggtggtg ccgtgcggct
    13261 ggggctggtg gggctgggca agatcgcccg cgatcagcat gtgccggcca ttgccgccaa
    13321 tccgcgcttt gcgctggtgg ccacggccag cccgcacgga ggcctggatg gcgtggccgc
    13381 ccatgccgat ctggccacta tgctgggcgc cgggcacggg ctggatgcgg tggcgctgtg
    13441 cacgccgccg gcggtgcggg cgggcctggc gcggctggcg ctggaggcgg ggctgcatgt
    13501 gctgctggaa aagccgccgg ccgccggcat cacccaggtc gccgatctgg aggcgctggc
    13561 cgcgcgtggc gggtgcagcc tgctgaccac ctggcacagc cgcgagacgg ccgccgttga
    13621 tgccgcgcgc gcctggctgg cggggcggcg ggtggaagcg gtgcgggtga tctggcgcga
    13681 ggatgtgcgc cgctggcacc cggggcagga ttggctgctg gccgccggcg gctttggcgt
    13741 gttcgatccg gcgatcaatg cgctctccat cctcacccat atcctgcccg agccgctggc
    13801 gctggaaagc gcggagctgg gcattcccga taaccgcgag gcgccgatgg aagcgcgcct
    13861 gcgcctgcgc catggcgggg tggcggcggt ggattgtgcg ctgtcgatcc tgccgggcgg
    13921 cgcccagcaa tgggatatcg ccatcgacac cgatgccggc gccctgctgc tgagcgaggg
    13981 cggccaccgg ctggcggtgg atggcactgc gcaggccttc gatgccaatg ccgaatatgc
    14041 ccgcctctat gcccgctttg ccggcatgat cgatgccggc acgcgcgatg tggatgcacg
    14101 gccgctggcg ctggtggccg atgcgatgat gctgggcggg cgctatcgcc tgccggcctt
    14161 tgcgttctga gcgggcgatg agcaccccct tgctccccct tcgccgccgc gctgtgctgg
    14221 cagggtctct ggcggctggt gccgcgatgg gggcagcgcc gctggcgcgc gcggccctgc
    14281 cgtcggcggg cccggtgctg cgccgcgcaa cgcggctggc cgagggctgg cgctttcatc
    14341 ttggccatgc cagcgatccg gcgcgcgatt tcggttttgg cgcctggcag cgcagcttcg
    14401 ccaagcccgg cgccgagctg accagcgctg ctgccgccga tttcgacgac agcggctggg
    14461 aagccgtctc ggtgccgcat gactgggccg cggggctgcc ctatgcgccg cccgcccagg
    14521 tgcccaaggg caaggaggat tttgccgccg ctcatggttt tcgcgccatc gggcgggatt
    14581 ttccgggcca ttcggtgggc tggtatcggc tgaagctgcc gctgggcaag gccgacaggg
    14641 gccgcgcggt gtggctggaa ttcgatggcg tgttccgcgc cagcatggtc atcgtcaacg
    14701 gctatcatgc cggcggcagc accagcggct atgccccttt ccgcgtcgat atcgccgatt
    14761 tcctcaatga tgacgacacc cccaaccagc tggtcgtgcg cgtggatgcc agccttggcg
    14821 agggctggtt ctatgaaggc gccggcatct accgccatgt gacgctggtg tcggccgccg
    14881 cgcagcatgt gccgcaatgg ggcgtgtgcg tgcgcgccag cccgcggggc accggtgccg
    14941 aggtggcggt ggcaacggat atcaccaatg ccgcagccca tccggcgatg gtggcgctgc
    15001 gccagcgggt gatcgccgcc gatggccagg tggtggccga aagcgagagc gcgcccgccc
    15061 cgctggccgc cggcgctgcc accacgctgg cgcaggcgct ggcctttccc gctgcccgcc
    15121 tgtggtcgcc cgaggatccg gcgctttatc ggctggagac caccgtgctg acagatggca
    15181 ccccggtgga ccgggtggag acgcgctttg gcgtgcgttc actgcgcttc agcggacgcg
    15241 agggctttgt cctcaacggc cggccggtga agctgaaggg cgtgtgcaac catcaggatc
    15301 atgccggcgt tggcactgcc attcccgatg cgctgcacgc ctggcggctg ggggtgatgc
    15361 gcgagatggg cgccaatgcc tggcgcagcg cccataatcc gccggccgag gcgctgctgg
    15421 acctgtgcga tgcgcaaggg atgatgatga tcgccgaaac ccggctgaac accaccagcc
    15481 cggaagggat ggacgagctg gaccggatga tcctgtcggc gcgcaaccat cccagcgtca
    15541 tcctgtggtc ggtcggcaat gaggaggggc atcagggcac cccgcgcggc gcgcgcctgt
    15601 cggccgggct ggtggcgcgg gccagggcgc tcgatcccac gcggcaaacc acccaggcga
    15661 tggacagcgg ctggtatgag gaaggggccg cgcgcgtggt cgatgtggtg ggcttcaact
    15721 atcgcaccga ccagatcccc gccttcaagg cgaaataccc cgatgtgccg gtgatcggca
    15781 ccgaaaccgc cagcacagtg gccacccgcg gcgcctatgc caatgatccg gccgctcatg
    15841 tggtgcgcgc ctacgacacc gagcacccct ggtgggccgc caatgccgag ggctggtggc
    15901 cgatcgtggc ggccaatccc ggcattgccg gcggcttcat ctggaccggc ttcgactatc
    15961 gcggcgagcc gacgccctat ccggcctggc cctcggtttc ctcctatttc ggcgtggtgg
    16021 atatctgcgg cttccccaag gacaattacc actactaccg cgcctggtgg cggcccgatc
    16081 tgccgcaggt gcatctgctg ccacactgga catggccggg ccgcgagggc cagccgatcg
    16141 aggtgtgggc gcatggcaat tgcgcggcgg tggagctggt gttgaacggg cgcgggctgg
    16201 gccgccaggc aatgccggcc aatggccatc tcgtctggca ggtgccctat gcggccgggg
    16261 tgctggaagc gcgcgggctg gatgcggcag gccgcctggt ggcgcaggac agccgcgcca
    16321 ccgccggcgc gccggccggc gtgcggctgc tggccagcaa gccgcggctg gcggccgatg
    16381 gcgccgatgt ggcggtgatc cgcgccgaga tctgcgatgc cgccggccgc ccggtgccca
    16441 ccgcccgcac gcgcctggat tttgcgctgg aaggccccgc gcgcctgctg ggcaccggca
    16501 atggcaaccc caccgacacc acccccgatc atgccgccag ccgcccggcc ttcaacggtc
    16561 tggcgcaggc gatcgtgcaa tccaccgggg ccacaggcgc catccagctt gccgtcagcg
    16621 gcgagggggt ggcgccggcc cggctctcgc tcttcgctca cgcgctctga ccctcttcgc
    16681 tcacgcgctc tgaaaggcat catccatgtc ccttggcctt gcttctgccg ccctgctggc
    16741 cgcctctggc ctgcatctgg gcgccgatct gtccttcacc aacgagatgg aggcctgcgg
    16801 cgcccatttc cgcgaaaatg gcaaaagcgc tgatcccttc gcgctgctga aggcgcatgg
    16861 cggcaatatc gtgcgggtgc ggctgtggaa tgcgccggcc acgccctatt cggcctatgc
    16921 ggatgtgctg aagacgatca cccgcgccca tgcggccggc ctcgatgtgc tgctcgattt
    16981 ccattattcc gacgattggg ccgatggcga caagcagatc ccgccggccg cctgggctga
    17041 gctttccacc gatgcgcagg tgcaggccct gcacgactac accgccgtca tcctcgaccg
    17101 gctggccgcg gccgggcagc tgccgcaatt cgtgcaggtg ggcaacgaga cgaaccccga
    17161 gatgctgggc gggcgcaagg gcggcaagat cgactgggcg cgcaatgccc gcctcatcaa
    17221 cgccggcgtt gccgccgtgc gcgcggcggc caaggcgcat gggcagccga tcagggtgat
    17281 gctgcatatt gcccagccgg aaaacgtgat cggctggttc gacgaggcga ccgctgccgg
    17341 ggtgatggat tacgacatca tcggcatcag ctattatgcc aaatggtcgc gctataacct
    17401 gtcgggcctg ggcaaggtga tcgatgccgc ccaccgccgc tatggcgccg atgtgtgggt
    17461 ggtggaaacc gcctatccct tcaccaccgc ggcggccgac gataccacca accttctggg
    17521 cgccgattcg gcgatacccg gctatccggt cagccccgag gggcaggcgc gctatctgga
    17581 ggatctgacc agggtggtgg tggccaatgg cggcacgggg gttgtctatt gggcgccgga
    17641 ttgggtgtcc accggctgca agacgcgctg gggcaccggc tccagctggg agaatgccgc
    17701 ctggttcgat atcgcccacc acgaggcgct gccggcgctg cgcttcctgg gcgaggatta
    17761 cggcgcgaag aagtgagggg gcggggcgcg ctgctgcccg ccccttttcc ttgcccccgg
    17821 cacagcaaaa gggccgcgat tcgatcgcgg ccctttcgtt tggcgccagg ctgatggctc
    17881 agtatttgaa cgttacgtcg gccagatagg aacggccgta tttctcgtag cgggccagtt
    17941 cgttggggtc gttgtcgtag tagaagcggg cggcctggtt ggtgaggttg ttggcctgga
    18001 agcgcagcga cacctgcttg gtgagcgcat aggagatgct ggcgcccacc gtcgtctcgc
    18061 tggccaggcg cgtgagcttg gtggcatccc agccgaagat caccgtgtag gggctgtggt
    18121 gcttcaccgc cacgcgggca tccacgccat gggcggaata ccacaggtcg aactcgccgg
    18181 tgaaatcggc caggcccacg gccgggaagg ggttgtagct gggcgcctgt tccttgaggt
    18241 tcgaggtgac cagcgcgaca ttggtgtaaa gcccgaaatg ctgcagcccc ggcacgaacc
    18301 agaacggggt ggaaagcgca aattccatgc cggcgatata gccgcccttg ccattggcag
    18361 ggccggtgat ggcataggtg ctgccgccga tgttcaccgg atattgcgtg tagccgacat
    18421 tgctgtcgac atccttgtag tagccggcca gcgccagcac gccatccttg tggaagtaat
    18481 attccaggct gagatcgcct tgcgttgcca tgaagggctt gaggttgggg ttgccggccg
    18541 tgcccgacag cacgctgggc ggctggtatt gcaggttctg gctggcgcgc aattcgtcga
    18601 gcggcgggcg gctcatcacg cgtgaaacgc cggcgcgcag cttcagctca tgggtcaggt
    18661 cgaagttgag gttgaggctg ggcagggcgc ggaaataatg gcccggcgca tagatcggcg
    18721 cataggtggt gccggcggtg gtggtgagcc cctgaaacgc gctggagccg gtgttcacat
    18781 tgaccagccg cacgccgaca ttgccgctga acggcaccgc gcccacagcg cccgccagct
    18841 cggccatcag atagccttcc acatcgcttt cgcgcacccg ccagtattgg ctcggatcag
    18901 atgtggcgcc ggccaggttc agcgaggaga tcgcatcgta attggcgtag atcaggctgg
    18961 gcgtgctgaa gccgccgccg gccacgttga aggaggagaa gctgggcgtg ccggtgaggt
    19021 tgaccgtgcc gccgatcagc gaggtgaagc tcttgatgcg gctggaatag cgcaggcccg
    19081 catcaatcgc gctgaccagc ccgccatgca gcttgtagct ggcatccacc tgcatcgcgc
    19141 ccagcgtgtc caccagcttt tgcgggccgg tgagggcaga gccggcatag gactggccgc
    19201 tgccggcaag atcggtggcg gtggacacgc tgggcaccac gccggcggcg gtgttgtagc
    19261 tgagcacctg gggccacacg ttccattcct gcgcggccca ggtgttggtg cggcgggcct
    19321 gcgaatagga tccgtcgatc ttcacggtga gatcgccggc gcggtagcgg gcgttgaggc
    19381 cggtggcgag gaggttcttg tcctcggtgt atttggcgat gacgttggta acggtggtgc
    19441 cccagttggg gatggtggcc gccacgatat cgcgcccggc cagcgtgaag ccgccggcgg
    19501 tgtagagcga atcggggatc gtgccgcccc agtcgcccca gccgccatac cattgctgga
    19561 actggttctc gtcgatcttc acgtcggaat agaggaagtc ggcgttcacg tcccagtgct
    19621 cgtcgtcctt ccactgcagc gcggccgagg tgctccagcg cgtttcggtc agcgccttca
    19681 cttcggtctg cgcgccccag ggggcataga cggtctggcc gttataggtg ggcgggctgc
    19741 tgccggcatc ggcattgttg tagccccagc cctggaagga ggaatagccg ttgcgctggc
    19801 gctgataggt gccgcccaca gagaggccca gcgtgtcggt cagccgggtg acatactgga
    19861 tgctgccgcg cgcgcccaga ttgtcgtaat tggggatctt gatgccggca tcattgtaga
    19921 gcgcgccggc gcgcatcgtc agcttggggc cgacatagtc gagcgggcgg atggtgcgga
    19981 tgtcgatcgt ggcggcaacg cccccggcga tgagatcggc cccttgcgac ttgtagacgg
    20041 tgaccgagga gaccgtttcg gacggataga tctcccagcg cacattgcgg tcgggctcgg
    20101 aagaggcgac ctcgcggccg ttcaccagcc ccagcaccag ccgcgggccc aggccgcgca
    20161 ccgaggcctg gctgtcgttg ccgcgatcgc gcgtggcgtt gacgccgggc aggcgggcca
    20221 gctcgtcggc gatcgtcaca tcgggcagca cgccgatgtc cttggaggag atcgtctcgg
    20281 tgatggtggc cgagctgcgc ttggcctgga tggcatcgcg cagcgaggcg cgcaggcccg
    20341 tgacgatgat gtcgggcgcg gcgggcggct gggcggccgg gttatcggcg ccggtgtcgg
    20401 tgccagtgtc agcgccggtg ttggtggtgg cgtcggcggc gcgcgccggc atggcgccgg
    20461 ccaccagcgc aagcgccagc gtgccagaca gagcggtggt gtgaataggt atgcgaatca
    20521 cgaaatccct cccgttcgga tccttcgtct catttatctt ataaataggg tcaggcgtga
    20581 gggtgtgtca agacgatctg ctattttgca gatatatctc cgtaattcag caatatatgg
    20641 cagtgtgccg ggtcagccgg gggtgccttg cccggcctcg cgcagcacca gctggccgcg
    20701 cagcaccacc ttgcgcacag gcgcttcggc atgttccagc cggtccatca gcatggccat
    20761 ggcgctgcgg ccgatctcct cgaccggctg ttcgatgacg gcaaggccgg tggaaaggca
    20821 atccatccag ggctcgttgt cgaagccagc catggccagg cccggcggcg cggcggcgcc
    20881 cagcgcccgc accgcgcgca gggctgccgt cagcatcacg ccgttggagg cgatcagcgc
    20941 ctcgggcggc tgcggcgcgg tcagcagggc ggagacgatg cgttctgtgg cgccggcgcc
    21001 atggggcacg ggcaccgcct gagcagccag ccccgcgcgc gccatcgccg cttcatagcc
    21061 cagctgccgc tccagcccgg tggcgctggc cgcgccgaac aggccggcga tccgccgatg
    21121 gccactggcg gccagatggt ccaccagcag gccggccatc gcgccattgt cgagcaccac
    21181 ctgatcggcc atcacatgcg gcgctgtgcg gtcgatcagc acggtgggga agtcgaaagg
    21241 ctggcgggcc agctggtcga gcccggcgcg ggtggcggca aggatcaccc cggtcacccg
    21301 ctcttcctgc ataagctgca gatacatcgc ttctttttcg gggtcctcgt cggtgttggc
    21361 aaggatcacg cgcaggcccc tcgcataggc cagatgttcc accgcgcggc tgacggcggt
    21421 gaagaagggg ttgcggatat cggccacgat cagcccgatg gtgttggtgt ggcgcgatcg
    21481 caggcggcgc gcggccaggt tggggcggta tccggtggct tccaccgccg ccagcacgcg
    21541 cgcgcgcatc gcctcgtcca ccgcccggcc ggcaagcacg cgcgacaccg tggccggcga
    21601 cacctgcgct gccgcggcaa catccttgat ccgcaccacc atcgggaaat cgatctcctc
    21661 tcttttctct gcctatcctc accatggccg aacaaaaagc gcaagggcaa agcatttacg
    21721 ggattcgtct tgacaagaac gtgagaaatc gatttctgac gcatgatctg aaagcagagc
    21781 aagcatagag agggtggcag atgacgctgg aggcgttggc aatcggcgtt gcgccgaagg
    21841 atcttatccg tatcggcgcc gaagtggcgg gcaaggaagc gacgattcgc cagctgggcc
    21901 agatgctggt ggccgccggc tgcgtgagcg cgggctatga cgagagcatg gtgcgccgcg
    21961 aggggctggc caacactttc ctgggcgccg gcatcgccat cccccacggg ctgggcgagg
    22021 acaaggggct ggtacgccgc gatggcatag ccgtgctgca ggtgcccggc ggggtggaat
    22081 ggaaccccgg ccaggtggcc catcttgtcg tcggcatcgc cgccaattcg gatagccaca
    22141 ttgccatcct gcgccgcctc acccgcctca ttcaggatga ggcgcggttg gccgccctgt
    22201 tcgccaccag cgatgccggc gaaatcgccg ccgcgctgaa cgaggatggt gcgcccgcca
    22261 ccgcgcctgc cgccaccgat tatgccgagc agcgggcctg ggtgctcgat tatcccaccg
    22321 gcctccacgc ccgcccggcc acgctgtgga gcgaggccgc caaggccgcc ggcgtgccgc
    22381 tgcgcgtgcg ccatggcgcc gaaagcgctg atccgcgcag tctggtcggc ctgctgcagc
    22441 tggggctgaa ggcgggcgat gcgctcaccc tttccgccca ggggcccggc gctgccgagg
    22501 cgctggaccg cttcgtcgcc ctcatcgcca gcctcagcgc gcgcgagcag gccgaggccg
    22561 agcgcgccgc tgccaagcgc gccgccgccc ggcccgcgcg cggctggcag ccggccggcg
    22621 atctggcctt catccccggc gttgccgcca gcccgggcct ggccatcggc cgcatccatg
    22681 tgctggccgc cgccgagctg gacgtgcccg accggcccgt gggcctggcc gagggcgggg
    22741 cgctgctgaa cgaggctttg gtgcgcaccc gcgcccagat gaaggcgatg gtggacgaca
    22801 ctgcccgccg cctggggccg gccgatgcgg cgatctttgc cgcccaggcc gccctgctgg
    22861 aggataccga ccttatcacc cttacctgcc agctgatggt ggagggccat ggcgtggcct
    22921 gggcctggca tgaggcggtg cagcgcatgg cggggcaatt gtcggcgctg ggcaatccgg
    22981 tgctggccgc gcgcgcggcc gatttccgcg atgtggggcg ccgggtgctg gaacagatcg
    23041 atccggcgct gggcaccggc tcgctcgccg atctgcctga tgagccggcc attctggtgg
    23101 cgggcgatct ttcgccctcg gatacggccg cgctggatgt ggcgcgggtg gccggccttg
    23161 ccacggcgct gggcgggccc acatcgcaca gcgccattct ggcgcgcacg ctgggcctgc
    23221 cttcggtggt ggcggccggc gctgaagtgc tggggctggc gccgggcagc ctggccatcc
    23281 tcgatggcga tgccggccgg ctgtggctgg ccccggacga agccgatatc gccagcgccc
    23341 gcgccttcct tgccgccgcc gcccagcgcc gcgccgccga ggaggccgag cgcggccagc
    23401 ccgccatcac cgccgatggc acccggatcg acatcgccgc caatgtgaac cggcccgatc
    23461 aggtgccctt cgcccttgcg caaggggccg aaggggtggg gctgatgcgc accgaattcc
    23521 tcttcctcga acgcggccac agccccgacg aggcggaaca ggccgccatc tatgccgaga
    23581 tggccgcagc gctggaaggc cgcccgctga tcgtgcgcgc gctcgatatc ggcggcgaca
    23641 agcaggtgcc ccatctcgat ctgccgcagg aggacaaccc cttcctgggt gtgcgcggcg
    23701 cacgcctgct gctgcgccgg ccagacctgc tggagccgca gctgcgcgcg ctctatcgcg
    23761 cggcaaaggc gggcgggcag tttgccatca tgttcccgat gatcacctcg gttagcgagc
    23821 tgctggccct gcgcgcccgc tgcgaggcga tccgcgccga gctggcagcg cccgtgctgc
    23881 cgatcggcat catgatcgag gtgccggccg ccgcggtgct ggccgatgtg ctggccgaac
    23941 acgccgattt cttctcgatc ggcaccaatg acctcaccca atatgccctg gcgatagacc
    24001 ggcagaaccc ggaactggcg agcgaggcgg acagcctgca cccggcggtg ctgcgcctta
    24061 tcgccatgac ggtggccggt gcccaaaagc acaaccgctg ggtgggcgtg tgcggcggca
    24121 tcgctggcga tcccttcggc gcggcgctgt tggcggggct gggcgtgtcg gaactgtcga
    24181 tgaccccgcg ggatattccc gccgtgaagg cgcggctgcg cggcgccgat ctggcgcagc
    24241 tggcattgct ggcggcccgg gcgcaggcct gcgccgggcc cgatgcggtg cgcgcgctgg
    24301 atgcgcaggc gcaggcgcag gcacaggcac aggcacaggc gccggcagcg ggggaggcgg
    24361 cctgatgcgc ctgctcaccc tcaccctcaa cccggcgata gaccttacca tctcgctcga
    24421 tcatctggtg ccgggcgaag tgcaccgggc gcgggccagc cgcagcgatc ccggcggcaa
    24481 gggcatcaac gttgccagcg tgctggccga ctgggggctg cagccggccg cccacggcct
    24541 gctggggcgc gacaatgccg cgccgttcga agcgctgttc gccgccaagg gcattgccga
    24601 caaatgcctg cgcgtggccg gcgccacccg caccaacctc aagctggtgg atgcggccga
    24661 caccaccgac atcaacatgg atggccccgc catcacgccc gaggaagccg ccgccgcgct
    24721 ggccggggtg gtggccgagg ccggccccga tgcgctggtg gtgctggctg gcagcctgcc
    24781 tgccgcctgc ccgcccgata cctatgccga ggcggtggcc gccttgcgcg gcaggggcgc
    24841 gcgggtgctg ctcgatacca gcggggcggc gctgcaaaag gcgctggcgg ccgctgtgct
    24901 gcccgatgtg atcaagccca accggcgcga actggccgat tgggccggcc gcccgctggc
    24961 caccatgggc gatgtgctgc gcgtggcggg cgatttgcgc gcgcgcgggg tggcgctggt
    25021 ggtggtgtcg atgggagccg aaggcgcgct gttcgtgggt gccgggggcg ccttgctggc
    25081 gcggctggcg gcgggcccct tggccagcac ggtgggcgcg ggcgatgcga tggtggccgg
    25141 ccttgccgcc gcccttgccg agggcgccga tctgccccgc atggcgcgcc tggccacggc
    25201 ctttgcggtg gccaagctgg gccgcgcggg gccccatctg ccggaacggg cctgcgtgga
    25261 agcgctggcc gatgaagtga cgattgccgc ggccgaaacc atcggcgcag gcgtgcgagg
    25321 agaggacgaa tgagccggat ctgggcggtg gtgaatgccg ccggggcagg ggtgtcgggc
    25381 gtgttggcgg ccgaggcgct gcgcaaggcg gcccgcgcgc gcgggcagga tatcgagatc
    25441 gagctgatca ccgagcatgg cgcggtgaac cccatcacgc ccgcagccgt gcccgccggc
    25501 gcgccggtgc tgtgggtggg cgatgcggag gacggccacc tgctggcggc gcgcggcgcg
    25561 gccgtggcga tcggcgaggt gctggcagac cctgccggca cgctgaccgc cgccctggct
    25621 gcggcgccgg ctgcggcgca gccaatgcgc atcgttgccg tcaccagctg ccccaccggc
    25681 attgcccaca ccttcatggc cgccgaaggg ctgaccgagg gcgcgcgcca gctgggccat
    25741 gccatccgcg tggaaacgca aggctcggtg ggcgccggca cgccgctttc cgccgaggag
    25801 attgccgcgg ccgatatcgt catcatcgcg gcggatcgcg aagtggatcg cagccgcttt
    25861 gccggcaagc gcgtgtttgc cggcagcacc aagccggcca tcgccggcgg cgcccggctg
    25921 atcacccagg ccctggccga agccagcctg caagccgcgg cccccggcgc cgatgcgccc
    25981 gccccccggg cagacaagcc cggcccctac aagcatctga tgaccggcgt ttccttcatg
    26041 ctgcccttcg tggtggccgg cggcctgctg atcgccatgg ccttcgcgct gggcggcatc
    26101 cacgccaacg acgaggccgc cgccggcacc ctggcccacg ccctgttcac cattggcgcc
    26161 aagggcggct ttgctctcat cgtgccggcg ctggccggct atatcgcctt tgccattgcc
    26221 gaccggcccg gccttgcccc cggcatgatc ggcggcatgc tggcggccaa catcggcgcg
    26281 ggctttctgg gcggcatcgc ggcgggcttc attgccggct atgcggtggc ggggatcaac
    26341 cgcgcgctgc cgctgccgcg caatctggcg ggcctgaagc cggtgttgat cctgccgctg
    26401 ctgggctcgc tcgtcacggg cctggccatg ctgtatgcgg tgggccagcc ggtggccgcg
    26461 gtgctggctt tcctcaccga atggctgcgc ggcatgcagg gcagcagcgc gctgctgctg
    26521 gggctgatcc tgggcgcgat gatggccttc gatctgggcg ggccggtgaa caaggcggcc
    26581 tatgcctttt cggtggggct gatctcggcg caggtctaca cgccgatggc cgccaccatg
    26641 gtggccggca tgaccccgcc gctggccgtg gcgctggcca cccgcctgtt cgccaaccgc
    26701 ttcacgcccg acgagcgcga ggccgccggg gccaccgccg tgctggggct ggcgttcgtc
    26761 tcggaagggg cgatcccctt tgccgcgcgc gatcccttgc ggatgatccc ctcgctgatg
    26821 gccggctcgg cgctggccgg cgccatttcc atggccgccg gggtggaact caaggtgccc
    26881 catggcggca tcttcgtgct gccgatcccg ggcgctgtca gccatgtggc gctctatgct
    26941 gccgcgctgg tggccggcac ggtgttcagc accctgctga tcggcctgtt gaagcggcct
    27001 gccgccgccg cctgaatgcc cgctgtttgc cagaggcggt aagcccgccc cggccgttat
    27061 acgagagagg aatacccatg accgttcgcc atctggcggc caccctgctg gccgctgccg
    27121 cccttgctgt gccgctgcgc ccggcgctgg ccgaagcggc ccacgccgcc gccgccgaca
    27181 gcagcgccga taatgccgat gccgccgcgc ccgatcccca ggcgctgccg atgccgcctg
    27241 ccgcccagtg gcagccgctg gcgcgccagg gcatcagcct ctcgctcagc tacaccggcg
    27301 aggcggccgg caaccccacc ggcggcctgc gccacgccag cgcgctgacc aaccagttct
    27361 acatgggcgc ggatgccgat cttaacacca tgctgggctg gcagggcgcg gcgatccatt
    27421 tcgccgtgac cgaccggcgc ggcccctcgc tcagcaacac ggcgctgggc aacaatacct
    27481 cggtgcagga actgtggggc acgcagaaca cccatctggc catcgccacc atcgaacaga
    27541 agctgtggca tggcgcggtg gatgtggaga ttggccgcag ccaggccaat atccatttcc
    27601 tcaactcgcc gatctattgc aacttccagt cgaactccac ctgcggcaac ccgacgatgg
    27661 tgttcaagga cagcaacctc acctatttcc ccgcatccag ctggatgggc catgtgaagg
    27721 tgacgctggc gccagactgg ttcgcccatg ccggcgtcta tgaagtgaac cccgagcgca
    27781 aggcggccaa cgaccatggc tggagcctgt ccaccgccgg cgccaccggc gtggtggtgc
    27841 cgtgggaggc cggctatgcc catgagggcg gcgacgggcg ggcggccgcg cactacatcc
    27901 tgggcggctg gttcgaccgc gccgattatg ccgatccgct gcgcgacggc aacggcggca
    27961 tcgccgtgct caccggccag gcgccggcca cccgccatgg ccgcagcggc ctctatttcc
    28021 gcttcgaccg gcagatcacc cgcccggtgg ccggctcgca gcggggcctc tcgctgttcg
    28081 gcatcgtcat ggccaacacc agcgggcagg tggaggaaag cagcttttgg gaactgggcc
    28141 tgctgcagac cggcaccttc aaacaccgcg aggccgacac gctgggcttc atgatcaacg
    28201 accagcgctt ctcggatctg gcgatgcagc gcatggcggc cgcgcgggcc ctggccggcg
    28261 gcagcgatcc tgtccaccgc cacgaataca tgatggagct gacctatggc ctgcaagtgg
    28321 ccagcgccat ccgcgtctcg cccaacatcc agtatatcct ccaccccgac aataccggcg
    28381 cgccctatcg caccaccaac acgcccgaca cgctggtgct gggcctcaag ttcaccatcg
    28441 atgcgctggg gctggcgcgg atgggcctgc gctgaaggcg gccaaaggcg ccccgccccc
    28501 ttgcgcaaaa gcggcgcaaa gcgctgcggg caggggggca gggctgcctt gtgggcgcaa
    28561 atttgccact aaataaaggc aatattcgag aaattcccag cgcacaggaa cgccataccg
    28621 atgccgcaga tcgggggcgg tcctctcagc tggaatttga cgaatgcgtt ttatctcgaa
    28681 gatccgggtc gctgcgctgg cggttccggc cggtactctg ggcttgccgg cgctggcgct
    28741 ggccgattgt tccacctctg gcaccaccgt cacctgcacg ggcacgacat cgggcttctc
    28801 caccaccacc gccgacacgg cgatcaccgt caattcgggc gccacggtgg tggggccgct
    28861 ggccgcatcg ggcgatggca gcacgctgac gaatgccggc accatctacg aaagctcggc
    28921 cgtctatgcg gtgcagatgg gcgatgatgc caccgtcacc aacaccggca cgatctcgtc
    28981 caccagctcg tcctcgggcg ccggggccat ctcggtgggg gccgacagca ccgtttataa
    29041 ccacggttcg ctgaccgccg aaagtggcac ctatgccgtc tccttcggca gcaacggcac
    29101 cttctaccag gaaagcgatg ctaccgccga gctgaccggc aacatcaatt tcggcaccaa
    29161 caccggcagc aacatcgcca gcctctacaa ttacgacacc acctacggca tctatggcaa
    29221 tgtctatgcc tcgggcaatg tgcaggtcta taacgacggc atcttcgagg gcaatttcta
    29281 ccagaccgcg gttgccggca gcagcaccgt cactttcgac aatggctcgg cggccaccta
    29341 tgtcggcatc atccagaccg gcaacaacac caccctcacc aatgagggga cgatgtacct
    29401 ctattcgggc agcgtcatcg gctcctatta ttcctcgggc accgatggca cctccagcgt
    29461 gacgaacagc ggcacgcttt atgtcggcac cgtcagcgcg ccggccgcgc tcgacatcta
    29521 tggcgatttc acccagacct cggccggcac gctcaacatg gcgatcagcg gcaccggttc
    29581 tgccggcacc acctacagcc agatctacac ctcgggcacc gccaccctgt cgggcacgct
    29641 caacatcaac acctcctccg gctatgtggc cgacagcacc gtctacaagc tgatcgatgc
    29701 ggtgggcggc atttccggca atttcacctc cgttacctac aatggcaatt ccagctcgct
    29761 gttcctcgac tgggatatca tcggcgcggt ctcctcgggc agcggttcgg aacaatatta
    29821 cgaggtgagc gccacccaca ccggctatgc caatgccatg cgcagcaatg gcggcaccaa
    29881 caaccagatc gccgtggcca gcggccttga tccgctgatc agcatcgcca atgccgatac
    29941 caccagcggc gaggccaccc tgctgggcga ggtggatgcg ctgaacctgg cgcaggccaa
    30001 ggtgttcctc aattcggtga gcccggaagg ctatctggcc tatgcaacgg cgctgcgcga
    30061 tcaggccaac atgttcgccc gcacggtgga ccagcgcatg accgaccaga actcgaagca
    30121 ccccgaggat ggctggtggt tcaccaccat ggcccagttc gatttcgctt ccaccgcttc
    30181 cacccagggc ggctatcgca cgcgcgatca catgtttggc ctggccgccg gctatgatct
    30241 ttccggcccc aaccatgtga tcggcttggc caccagcatc tcgtgggatt cgctgcatta
    30301 tgcgctgaac agcctcaacg gcaccaaccg cgattgggcg gtggcccttt atggcgcgca
    30361 gaatttcggc cggctgcggc tttatgggca gatcgcctat aacatgggcc acctgtccac
    30421 caccaagacg atcaccatcg gcgactatac ctggaccgcc aacgcccgcg cgcccgaaca
    30481 tctgttcaag gccaccgcca accttggcta tacgtggagc ctgggccaat acaagctgga
    30541 gccctttggc ggcatcgact tcaattccgg ccgggtggca ggcttcaccg aatataacgc
    30601 gggcgctgcc gatctcaccg tctcggcgat gagcgccagc cacaccgatg tgctggccgg
    30661 cgttaacgtc acgcgcaaca agggcatctt ccgcccctat ttcaagatgg cctaccgccg
    30721 ccagctgacc ggcgaccgca acaccgtctc ggcctatttc gacgggcaga gctcgggcac
    30781 tttctcggtg acgggcctgc cggtgggccg cgacgagatc gatccctcgg cgggcatcaa
    30841 ctgggtgttt gatgatgccg gttcgctcta tgtcggctat cagggcacga tccgctcgaa
    30901 ctacaattcg gaaggcatca acctgggcat ccgcctcgaa ttctgagcct gtggggagcg
    30961 gggtggcccc gctcccccgg ttggttcaaa ccagctcggc cagcgccgag gcatcgaagc
    31021 ccttgaggcc ggtggcatcg cccgcgcgca ccttggcggc ccattgcgca tcgctgatca
    31081 gcgcccggcc cacggcgatg aggtcgaact cgtcgcgctc catgcgttcc accagctggc
    31141 caaggcccac gctggtggag gcctcgccgc caaaggccgc gatgaattcg cccgacaggc
    31201 cgacagagcc gacgctgatc gttgtggcgc cggtcagctt cttggcccag ccggcgaaat
    31261 tgaggccgtt ggcgccgtcg atctcgggga attccggctc ccagaagcgc cgctgcgagc
    31321 aatgcagcac atccgcgccc gcttccacca gcggcgtcag ccactggccc agctcttccg
    31381 ggctggtggc caggcgcgcg gcataatcct gctgcttcca ctggctgagg cggatgatga
    31441 tgggaaaatc cgggcccacc gcggcgcggg tggcggccac cacttcggcg gcaaagcggg
    31501 cgcgctcggc aatggtggtg ccgccatagc gatcggtgcg cagattggtg ccggcccaga
    31561 agaattcgtc gatcagatag ccatgggcgc catgcagctc caccgtgtcg aagcccagcc
    31621 gcctggcatc ggccgcggcc gcggcgaagg cggcgatggt gtcggcgatg tcggcctcgc
    31681 tcatcgccac cccgcgcgga tcgcccggcg cgttgaggcc cgaggggctt tccaccggcg
    31741 cctcgggcac ccattcggtc tggaagcttt tggtggcgcc ggtgtgccac agctgcgggc
    31801 ccatcttgcc gccggcggca tgcacctcgt cgatcacgcc ctgccagccg gccagcgcgg
    31861 cttcgccatg gaagaaggga atgcccggat cattgcgcgc ggcaggccgg ttcaccaccg
    31921 tgccttccga caggatgagg cccacgccgc cttcggcgcg gcggcggtaa taggcggcat
    31981 tggccgcgcc cggcacgccc tggggggcaa aggagcgggt catcggcgcc atgacgatgc
    32041 ggttggccag ttccagcgtg ccgatgcgca aggggcggaa gaggatatcg gaagagggca
    32101 tgggggatgt ccgttgctga agggaggcct ttaggtataa tcgggaaacc tggtgtcaat
    32161 aaggcacctc aaggcgccca ggtataccga aggaaaccgc atgcacgtct gccagcactt
    32221 ttccgtggat tgccccagcc ggcaattgtt cgaccagatc gccgacaaat ggtcgatgat
    32281 ggtgctgacg gtgctggatg ccggccccat gcgcttcaac gccatccgcc gccatctgga
    32341 aggggtgacg caaaaggcgc tgacccaatg cctgcgccgg ctggagcgca acgggctgat
    32401 ctcgcgccgg gtgatcgcgc gctcgccggt ggcggtggaa tatgcgatca ccgcgctggg
    32461 ctgctcgctg caccggccgt tcaaggcgct ctatggctgg acgctggcga acatggccga
    32521 agtggaagcg gcccgccgcg ctttcgacgc gcggcaggcc gcctgagcga tcactgcacc
    32581 agatgggcgc tttgcaccgt cagcgcgaac agcgcgttga gcgcggtgga aatgctctgg
    32641 tcggtggcca cctgatagaa cagcgccgaa ccattgccgc cggtggaggc gcagctttgc
    32701 agggcggtgg ccacctggtc ggtcgtgccg gcggtggtgg aggtgtagtt gctgggggcg
    32761 ggcagatatt tggccaccac cgattgcgac caggaatcgc catcgagcga atcgggcaga
    32821 tattgcgtgt agaggatggc gatcttgatg ccgcggctct tgatcgtgct gcacacggcc
    32881 agatcgctgg cgttgagcgg gctgaccgtg cggctgccgc tgaccgcctc gtcggacatg
    32941 ccatcggtga cgaggaagag cacttgctgc gggctgctgg cggtggaccc atcgcccggc
    33001 gctggcatca gattgttcat gtaggtcagc atgttgagga attccgagcc ctggtcgccg
    33061 ttcgaatagg aggaagtggg ccagttgttc gaataccaga aatcctgcgc cgcccacaga
    33121 tcggggatct ggctggcggt catgttcttc acatcggcca tggcactggt cagcgcggta
    33181 acgggcgtgg tctggctgtt gtaggtgtgg gtccagtcga atttgaacac ctgcatctga
    33241 taggccacgc tgttgttctt ggcggtggtc tgggcaaagg ggatcagcgc ctgggcggcc
    33301 gatcgctcgt cgtcgatgcg caggttcacc gtcgatcccg aagggaacag gatggtgtaa
    33361 ttatgcgtca gccaatagcc gtcggcccaa tagcctgcca cctggttgct ggaactggcc
    33421 tgggtgccgc cccagccgct gccgcagcgc gtggcctgat agacgccgcc gctgctgctg
    33481 caatagagat agcccgacaa ctggctgccg ctgctgttat aaaccgcgcc ggtgctggtg
    33541 ttcaccttgt agtagttggt ggtggtgccc gaggaataat agttcgacag caggaagatg
    33601 ctggtgctgc cattatacac atagggcttg ccgccattgg ggttctgctc gtggcaggca
    33661 aattcgcagc catagggggc gttggtggtg gcactggtgg ccgtggcgat ggccgacagc
    33721 ccggcgctgg tggagggcag cagcatcgag ggcgaattgt cgagcatcag gtagaaattg
    33781 atgtagggcg cgccggtggc accggccgag gacgtgccgc tgatcgtcag cgtggaggcg
    33841 ccgaggatgc cggcgaacac gttcttcgac tgggctgtcc agttgagcac cgccgtgcgc
    33901 ccgttgttga gcgtgccggt gagcgtgacg gtggtgcccg agccataggc gatgctgccc
    33961 gaaagcccgg tgagattgcc tgtttgcgcg gcgaaattgg cttccatctg ggcgatcagc
    34021 tgggcctgga actgggcatt gctgaggctg ccctgggatt gctgcatggc ggtggccgac
    34081 accaccgaca aggtggccgc atcggccgcg gcgttgatct gcgtctgcag cttcatcgcc
    34141 cggctgtagt ctatgccaaa gcccaccgcg aagatcatcg gcacgatggc aaagcccatg
    34201 atcatcagca cattgccgcg attgtcggcg ctgaggcgcc gggccaggtg gaggacgggg
    34261 cgcagcaggc gggcaggaag ggacaggtgg cgcatggcaa gggtccgaag ggcgatcagg
    34321 aggtgaccag cgcaatgctg gcggacagcc gcgggctcat gatgatcggc tggtagaagg
    34381 tgatggtgct ggcagagcca taccagaagc tgggcgtgta ggcgtagcgc acctcgccat
    34441 agatcaggca gccgccatcg ccggtaaggc tggtggcggt ggtgcagatg ctttgcttgg
    34501 tgctgccgga aggcagcagc aggctgggga tgaggccgct ggtggtagtg atcgaggtgc
    34561 cggcggtata gccgctcgac agggtggagc cggaatagct ctggctccac acgacgatga
    34621 catgggtgct gtccaccacc tgcacctggc tcagcgtgat taccgcattg ctggcggcat
    34681 agggcgccag cacctgctgg gcggcggcca tcagcgtgcc gatataggcg gcattgctgc
    34741 tgttggtgga aagataggcg ctgcgggtgg caaggtctgt cagcgcgcgg gcggtgatcg
    34801 ttaccttgcg gctgcaggcg atcatgtcgg acatgacaaa ggccagcatg tagagcgcga
    34861 tgagcaccgg cagggcaatg gcaaattcca ccaccgccac cgcccgtcgg ctgtgcgcca
    34921 gccgggccag ggccgcgggc agccggatgg gccagcgcca gggcaggcgg gcggccatca
    34981 gtagccctcc gttttcagca ccgaggtgga cagcagatag cgctggttgg tgccgccatt
    35041 ggccaaggtg aagcccagcg gggcagacag ggtgggccag atgtagagca ggcgcagctg
    35101 cacgatcgcc ccttgcgtgc cggtgcaata gttgaaggcg gcggtgatgc cattggcatc
    35161 ggcgctgccg gccgccacgg agctggtggt gcaggtggcg ccggccgcgc tgctggtggt
    35221 taccgtgtag gtgctggcag acatcagcgg ctggctggtg ctggcctggg catagctcgt
    35281 caccgtgttg atatcgacat agagattgct gcaggacatg aaggccggca gcagcgcgca
    35341 ggtcttctgg cggaaggtgg ccgcgctcca ggcgccgttc tgggcctggc cggtgaggat
    35401 atagcgcgcc gcgccctcgg tggcggtttc cagctgctgc tgggccagga agatgaaggc
    35461 gatctggaag attgccacta ccagcgcaat gaaggccggc gcgacaaagg cgaattccag
    35521 cgccgcgttc ccgcgcgaat cgcgcatgcg ggcaaggctt gcccatctgc cgcaaatgct
    35581 gaaaatatcc cgcctccgtg agcgtttttt gtgcaaaacc cagcgctatg cctagcagcc
    35641 aatggttaag cggcgcttgt cgaagggcgg ggaaaggccc gggggagggg cgcgcgttca
    35701 atcttgcgcc gggcccggcc cgccgctagg ccgccccgaa ccatggcgca gatgagggga
    35761 gcagcgcccc gtccggccgg cgcccggata acacgggccc ggataacagg cgccgggata
    35821 acaggagatt gccgcttgcc acctgccgaa ctgctgctga tccgccatgc gccggtgctg
    35881 gccgatggcc ggctggccgg gcggcgcgat gtggcagcgg tgctgcccga tgcggcagtg
    35941 ctggcggcgg tgcgggcgct ggccgggccg gtggaccggg tgatcgccag ccccgcgcgc
    36001 cgctgccggg ccaccgccgc cgccctctgg cccgaggccg cgctggtgga ggatgcgcgc
    36061 ctgtgggagc aggatttcgg cgcgtgggaa ggcctgccgt ttgccgatct gccggatctg
    36121 ggcgatctgg cgccggctgc gctcgccggc cacgccccgc cggggggcga aagctttgcc
    36181 gatctgtgcg cccgcaccgc gcccgcgctg gaggcatggg cggccctgcc cgggcgcagc
    36241 gcccttgttg cccatgccgg cacggtgcgc gcggctttgg cgctggcgct gggggcggcg
    36301 gccccggcgc tggccttccg catcgatccg ctctcgctca cccggctgca gccttgcggg
    36361 ggccagggct gggcggttgg cggggtcaat ctggtggcgg catgctgagc gcgctcgcct
    36421 ttcatgccgc cgccctgctg gtggcgctgg cgctggatgc gctgctgggc tggccggcct
    36481 ggctttatgc ccgcattggc catccggtca gctggctggg ccggctgatc gctgcggccg
    36541 actgtgcctg caaccgcgag ggcgccgctt ttgcgctgcg gcgcgcggcg ggcgtggcgg
    36601 tggcgctggg ggtgatcggg ctggcggccg gggctggctg gggccttgcc gggctggtgg
    36661 ccgggctggt gccggcgggc tggccgggcg tggtgttgct gggggccctg tgctggccgc
    36721 tgctggcggc gggttcgctc catgcccatg tggcggcggt ggcgcggccg ctggcggcgg
    36781 gcgatatggc cggcgcgcgc caggcggtgg cgatgatcgt cgggcgcgat cctgatcggt
    36841 tggatgcggc cggcattgcc cgcgcggccg ccgagagcct ggcggaaaac acctctgacg
    36901 gggtgatcgc gccgctgttc tgggggctgc tgttcggcct gccgggcatc gccggctaca
    36961 aggcggtcaa cacgctggat tcgatgatcg cctatcgcac cacgcgccac cgcgccttcg
    37021 gctgggccgc cgcgcggatc gacgatgccg ccaacctgct gcccgcgcgg ctgacaggcc
    37081 tcttctatgc gctggccagc ggtcggccat gggcggcgct gcgctgcatg gcggccgatg
    37141 cgcgccatca ccgctcgccc aatgccggct ggcccgaggc ggcgatggcc ggcgcgctgg
    37201 gcattcgcct gtcgggcccg cgcctctatg ccgaggggat gagccaggat cgctggctga
    37261 acggcgccgc gcccgatccg gtggcggccg atctggccgc ggcgctggcg ctgtttggcc
    37321 gggtggtggg gctggcggcg ctggggctgg cgctgctggc ggttggcgtg ggggggatct
    37381 gatgcgcgat catggcggca atctcgatgc ggcgcgggcg cgctttggcg gcgaggggtg
    37441 gatagacctg tccaccggca tcaaccgcgt gccctatccg ctgccgccgc tgccggccga
    37501 ggcgtggacg gcgctgccca ccgccagcgc ccgcgctgcc ctgctgcagg ccgccgccgc
    37561 cgcctatcgc accacagcgc cgatggtggc gctggccggc gcgcaggcgg cgatccagct
    37621 gctgccgcaa gtggtgcagg gccggcatgc ccgcgtgctg gcgcccacct acaacgagca
    37681 tgccgcctgc cttgccgctg ctggctggca ggtggcgcaa gtggccgatg cggccgatct
    37741 ggccggcgcc gatatcgccg tggtggtgaa ccccaacaac cccgatggcc gcgccctgcc
    37801 gccagaggca ctgctggcgc tggccgggca ggtgggcctg ctggtggtgg acgaaagctt
    37861 tgccgatccg gtgccggacc tgtcggtggc gccgtgggcg ggggcgggcg tggatcaggg
    37921 gcggctgctg gtgctgcgct ctttcggcaa gttctatggg ctggcggggg tgcggctggg
    37981 ctttgcgctg ggcgatggcg cggtggtggc agcgctcgat gcgctgtcgg ggccctggcc
    38041 ggtgtcgggg ccggcgctgg cggtgggcgc gcaggcgctg gccgatggcg cctgggcggc
    38101 cgccaccagc gcgcgcctgg ccgccgaagg tccgcgcatc gatgcgctgg cggccggctt
    38161 tggctggcag ctggccggcg gcaccgcgct gttccgcctg tatgacacag atgacgccga
    38221 ggccgcgcag gcgcatctcg cccgccagcg catctggagc cggatcttcc cctggtcgcc
    38281 ccggctgatc cggctgggcc tgcccggcga tgcggcggaa tggcagcgcc tggccgaagc
    38341 cctggcgccg cctacaccgg ccagtcgcgc gtctggctga aatcggcgcc gcaccgttgc
    38401 ccgcgcggca ggtagggatg ttcggccagg aagccatcgc tggccgcatc cgtgcgcgcc
    38461 cagtcgatga tctcgcgccg cttcaggatg cgccattcgc catcacggca ggcatattca
    38521 tcgacatagc gcccggccac catcagatcc tgcgcttcgc cttcctgcag caggcggtgc
    38581 caggcgtaga aatagatctc gccgctggcc gcatcgccgc gcacatcgat gtcggtctgg
    38641 gcgatgatgt gctgggtgcc ctccatgccg gccagcagat cttgcgcgaa atcgacgaat
    38701 tcggccgcgc tgccggccat cagcccgcaa tccacccagg catccgggtg gaaggcggaa
    38761 cggtgcagcg cggcatccag ccggtcctgc ccgcgcatat agcgcatcag cgccctgccg
    38821 atatcgcgcc gggcggccag atcgcgcact tcggcttcca gatcgaatgt gtctttcatc
    38881 atgcccctca tgccgacaga tacttgtcca ccacctgctg gaagtggcgg atgcgcactt
    38941 cctgataatt gcccagctgc tgcacgccgg tggcgctggc gcggaaacct tcctgctggg
    39001 cgcgcagatt gtcggtatcc tgatcataga ccgcgcccat gccgggatcg aagccgggcg
    39061 ccgaggcata gctttcctcc tcgcgcacgc ggtagggttc ggccggctct ggcgctgcgc
    39121 cctgatcggg gctgggccgc aggaacagga tctcgaacag ggtgcggttc ggatccagcc
    39181 cgatggggcg gaagcgatag gccatcggca gcgaaatgcc ggggaacagc accatgttgg
    39241 ggaagacgtg atattcgatc gtgtcgatca tctcgctgtc ggaaaagcgc gagaggtcgc
    39301 agccatattt ctcgcccatc acctggcgca ggaagcgggc catcaccgtg cgcgcgcttt
    39361 cgccctcggc cacggcaagg cccggatcga gcaccgagcg atcgcccagg atcatcgtgg
    39421 aaaccagctc ctgctcgctg agcggctggg ccagatgcgg cgaattgatg ccgctggccg
    39481 catagaagcg cgtcacatgg tcggaatgga tgtcgtattg cacattggca tcgcccacgc
    39541 ccttcatcag ctgggggtgg gtttccagca cgtgatagga ttcgaggaag gcctcctgcg
    39601 cggtcttcca gttgcagggc agctccttgg ccacatgcag ttcgacaaag cgcttgtcga
    39661 tgtcccagtc gcggaaatgt tcgggcaggg gcgcgaggaa ttctgcgagc gagggcccct
    39721 cgagtgccgg gttgatgaag atgaagccgc cccaatggcc cacgcgcact tcgggcaggg
    39781 ccgttttatc cgcgcagaca tggccgaaat cccacgcgca gggcaccgct ttcagcgcgc
    39841 catcattgtg ccacgtccag ccgtgatagg ggcagcggat ctcgccggca gagccgaagc
    39901 cctcggtgcg cttgaacttg gtggagcggt gcaggcagga attgacgaag cccttcacgc
    39961 tgccatcctc ctgccgcacg acgagatagg acagatgcgc cacctcatag gtgtaatagt
    40021 cgcccggctc ggggatatgg tcctcgcggc acacccattg ccagctcttc ttccagatct
    40081 tctcgatttc ggccgcgaag atcgcctggt cgtaatagcg atcgacgctg accggcgcgc
    40141 tgccgagata ggccggctct tcatgggcca gcaccggcgg cgggggcagg gcatccttcg
    40201 ccacgatctc ctgcacggaa ggggccgggc agcgggcgct ggcaaggttg gcctcgccct
    40261 gcgcatcagg caggccatgg gcgcggatgg catcaagcat cgggtctctc ctgggcgtgc
    40321 cgtggtaaac cggcagttgt gcctgtttta tggagagggc gccggccaag aacctcgcgt
    40381 ttgtgctagg aatggagcgg gcttcagcgc ccggcggggg cagcgcgcag gcggttcacc
    40441 acttcgggcg catcatgcag ccgcacatag agcacgccat cggtccccgg ccgcggcacc
    40501 ggcaggctga agccgggata gccctcggcc acgctggtcg ccttgtcgaa atccgccgtg
    40561 gcagacagcg aggcgagcag atagagcccc tcgcccgcca gcgtgcaggg gccggtggcc
    40621 gggcaggaga ggcgcttcag ccggggcagg cgcaccagcg tgcccaggtt cagccagtct
    40681 ccggcaacgc ccgcgcgcac cagccgcgcc cgcaaggtgc caaaggccga tgcccccagc
    40741 gccctggccg gcgtgaggct ggccaccatc gctgcgggat cgaccagcgt gaggccgctg
    40801 cccagcgtga ggcgggtgct ggcggtgcca tcggcggtgc tcacttccac cacttcgcgg
    40861 ccggtgaaac gctcctcgcc gctggccttc agggtgaagt tgagcgcggc atcggcgggg
    40921 atctcggccg gattgtcgag cagcagggcc agcggcggcg cctcgcccgc tgccgggctt
    40981 tc
//
LOCUS       DASRYE010000084        16641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_216614,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000084 DASRYE010000000
VERSION     DASRYE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16641
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216614"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFF98_04665"
     CDS             complement(<1..225)
                     /locus_tag="VFF98_04665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014694.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the epsilon subunit is part
                     of the catalytic core of the ATP synthase complex; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZU62954.1"
                     /translation="MSGLIALAISTPERLVVEAEVRSVRAMDESGSFGLLPGHADLLT
                     VLPPSVLRWRDAAGAEGFCALRGGVLTMTGG"
     gene            complement(222..1658)
                     /gene="atpD"
                     /locus_tag="VFF98_04670"
     CDS             complement(222..1658)
                     /gene="atpD"
                     /locus_tag="VFF98_04670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZU62955.1"
                     /translation="MTEAVSEQGEARGTVVAVRGAVVDIRFEGGLPLLEEAIRIALPA
                     GAILAEVQAHLDPHTVRAIALAPTTGLARGTPALATGAALTAPVGDAVLGRLLTVSGD
                     VGDGGAPLAEDTPRWPIHRAPPPLAPSSGDTERFITGIKVIDLLTPLAQGGKAAMFGG
                     AGVGKTVLVMELIHAMVSSYQGLSVFAGVGERSREGHEMLLDMRGSGVIERTVLVYGQ
                     MNEPPGARWRVPMTALTIAEYFRDAQHRNVLLLMDNVFRFVQAGAEVSGLLGRLPSRV
                     GYQPTLASEVAALQERIAPAGGAAITAIEAVYVPADDFTDPAVTTIAAHIDSMIVLSR
                     SMAAEGMYPAVDPIASSSVLLDKDVVGEEHARTAIEVRRALEHYRELQDVISLLGMEE
                     LGAQDRLIVGRARRLQRFLTQPFTVTEAFTGTPGRSVALADTIAGCRAILDGECDSWR
                     ESSLFMIGTLAEARAKEEAANAAEGVPA"
     gene            1810..2538
                     /locus_tag="VFF98_04675"
     CDS             1810..2538
                     /locus_tag="VFF98_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZU62956.1"
                     /translation="MVRLFHISDLHFGAEDRAALGWFAQVVAEEKPCGIICTGDLTMR
                     GTVREFNAAADYLGRLAAPVHIEPGNHDMPYYWEMGARLRHPFRRFGALRAKAHRALP
                     LADVAVVGLSTVVPAQWRLNWSKGCVRETVLATALHELAHAGPQALKLVACHHPLRDA
                     DTHGSGSTRGGGRALAQLAAAGAHAVLSGHVHDPFDMMTQTPCGPIRMIGAGTLSERT
                     RASRPSFNQLDWDGTLRVTARHHG"
     gene            complement(2603..4123)
                     /locus_tag="VFF98_04680"
     CDS             complement(2603..4123)
                     /locus_tag="VFF98_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZU62957.1"
                     /translation="MADMVTIREEDLIESVADALQYISYYHPMDYIRALGDAYEAEQG
                     PAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFIQWGQNCRLESEKSLQDVVDEGVR
                     RAYLNPENKLRASVLADPAFTRRNTKDNTPCVLHVDMVKGSKIHVDVAAKGGGSENKS
                     KFKMMNPSDSIVDWVLEMLPQMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEQIDMA
                     QLKERGPQNDLEALRIEIFDKVNALGIGAQGLGGLATILDVKIMDAPCHAAGKPVAMI
                     PNCAATRHAHFTLDGSGPSYLETPKLDEWPKVHWAPDAAAKKVNLDTLTAEEVQSWKQ
                     GDRLLLNGKMLTGRDAAHKRIADMLAKGEELPVEFKGRVIYYVGPVDPVRDEVVGPAG
                     PTTATRMDKFMDTMLGLGLLACVGKAERGPAATQSIANHKSAYLMAVGGAAYLVARAI
                     KASKVVGFADLGMEAIYEFTVEDMPVTVAVDSEGKNVHQLAPAVWKDKIAKEHLLA"
     gene            4481..5077
                     /locus_tag="VFF98_04685"
     CDS             4481..5077
                     /locus_tag="VFF98_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZU62958.1"
                     /translation="MTTTDTAHASFDTARRAMIDSQLRPSGVNEPWVLTAMAATPRED
                     YVPESARAAAYIDRALPLGQGRFLAPAVVQGRLLGEAAPTQADRVLVITGGTGYLTAL
                     VSQLAPNVDAVDAADLSAIPGSGYSLIIIDGAIEVLPDALADKLAEGGRIATGLLEGG
                     VTRIATGEKLAGKVVLVALSDLPVPVLPEFAAPKHWSF"
     gene            5080..6555
                     /locus_tag="VFF98_04690"
     CDS             5080..6555
                     /locus_tag="VFF98_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZU62959.1"
                     /translation="MRAAARVSCRLAILALGASPGAGHAEDLRAALAAAYAANPTLAA
                     ARETLRATDEGVAIARADGLPSASASVSETENLVQSQLSLGDAPRALALAGTLSVPVY
                     SGGAVRNGMKAADRRVLAGRADLAGSESGLFAQVVAAYMDVRLGEETLRLNRAEVETL
                     RQTQRATADRFHVGEVTKTDLAQAQARLALAEGSLRSAEANLVQARETYGQLVGHAPA
                     ALADPPPLPGLPASVEEAEAHALADNPDLAAARERSRAAAYDTRAAGAARLPKVSLFA
                     DAGYSDYLGGLKVLGYPSAAFPQSFTSADVGVRATIPLYQGGRPGAQIRQAEAREGAA
                     QDNEIAAERQVIAQLRSAYASWQAARAIIAYNETAVAAARLGLQGVRAENGVGNRTVL
                     DILNAEQELVSAQVQLATARRNAYVAGFSVLAAMGRAGARDLGLDGGALYDPALHYRQ
                     MRRSLGDAPGHQGASAPPQPASTPARTTVDTPPQTGTIEGG"
     gene            6630..7184
                     /locus_tag="VFF98_04695"
     CDS             6630..7184
                     /locus_tag="VFF98_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HZU62960.1"
                     /translation="MRQPGEPAVEDLLESIKKVIARDNQVTGQAGARAGGAEQRRLGS
                     SAGLAAAHDLRGLAQAAPATAFADDVLDLAMSAQFLDDHHEDDMHNYGHDNHGHDDGD
                     EEDKALIAPEARASMRESLAALAMLAQPGKAPQIVRSGETSLEELTRELLRPALAQWL
                     DEHLPALVESMVAAEIARIVGKKA"
     gene            7265..7696
                     /locus_tag="VFF98_04700"
     CDS             7265..7696
                     /locus_tag="VFF98_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZU62961.1"
                     /translation="MAKPLPHQLDSQRYPHRHLVPTRFSDLDPNNHINNVALAGIIED
                     GRVRLHRDAGWYAPELGVQIMLASLTVEYLAQSHYPLDITVLSGVGRVGRSSLDVEQL
                     VMQEGLCVAYARSVVVFVEDGAAVPLPGDVLARLDRWKLTQ"
     gene            7693..8076
                     /locus_tag="VFF98_04705"
     CDS             7693..8076
                     /locus_tag="VFF98_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZU62962.1"
                     /translation="MTARQEITRAEDALAKLAPGHDGPVRHIFLCAEPQKGECCTPDV
                     GSAAWAYLKKRCKQLGLAEAGIHRSKADCLKLCAAGPVAVVWPEGVWYHSCTAPVLER
                     ILQEHLIGGVPVAEYRLHAPCKAGS"
     gene            complement(8073..8435)
                     /locus_tag="VFF98_04710"
     CDS             complement(8073..8435)
                     /locus_tag="VFF98_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62963.1"
                     /translation="MPRPDPLPPPKARPSPLDNPAVAAHAWARFRRLMRLMFLVTTGT
                     VLIALALLYKQVGLVSVHFYIAVAVGISFAMLLTAGLMGLVFLSAGTGHDGAVGTPAE
                     PGDEDKDENRDSDEDRDA"
     gene            8550..8978
                     /locus_tag="VFF98_04715"
     CDS             8550..8978
                     /locus_tag="VFF98_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZU62964.1"
                     /translation="MTIARLLERRTSPVIFCSIHTPVREAVTLLAERRIGAVPVMDGD
                     AVAGIFSERDVIYQLGARGPGMLDLNLGQVMTSPAITVSPDTGVLEALSLITRRRIRH
                     LPVLRGGRMVGFISIGDLVKYRIDLIEAEAEAMRTYIQTA"
     gene            9053..9385
                     /gene="erpA"
                     /locus_tag="VFF98_04720"
     CDS             9053..9385
                     /gene="erpA"
                     /locus_tag="VFF98_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZU62965.1"
                     /translation="MSEMAITLSPSAAARVAWIAEKQGQPAILRLAVEGGGCSGFQYR
                     FGLAEAVAEDDTVSETDGVKLVVDAVSLDLVAGCVVDYVESLGGAAFRVENPQATAGC
                     GCGSSFAV"
     gene            9396..10169
                     /gene="xth"
                     /locus_tag="VFF98_04725"
     CDS             9396..10169
                     /gene="xth"
                     /locus_tag="VFF98_04725"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZU62966.1"
                     /translation="MKIASFNINGIKARLPRLLEWLAETRPAVACLQEIKTADEGFPA
                     EEFEKIGYQAIWHGQKGFNGVAILADGVQPVEVRRGLAGEPEDEQSRYLEADVFGLRV
                     AGIYLPNGNPQPGPKFDYKLRWMERLRARMAEIAAEEVPAIVTGDFNVIPRDCDIWSP
                     KAMADDALMQPQSRDAYATLMHDGWTDAIATLNPGGGVWTYWDYQAGAWQKDHGFRID
                     HALLSPELADRLVAAGVDKAHRGREKASDHAPIWVELRD"
     gene            complement(10257..10889)
                     /locus_tag="VFF98_04730"
     CDS             complement(10257..10889)
                     /locus_tag="VFF98_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HZU62967.1"
                     /translation="MRFIFNKASLITLAATLAVIAFGAAGSGAAAQKNATALAQAEPV
                     NLLPQAGATSAAQEQTLSGQPEAHDDASASEDLASLVAEAPQADALPKELNCLASAVY
                     YEARNETLSGQLAVARVIVARSHSGRFPASYCGVVFQHGQFSFVHNGNLPETSHASHA
                     WRNAAAIAAIADKGSWDSPVEGALYFHARRVSPPWHRERIARVDNHVFYR"
     gene            complement(11085..11426)
                     /locus_tag="VFF98_04735"
     CDS             complement(11085..11426)
                     /locus_tag="VFF98_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HZU62968.1"
                     /translation="MSARLPAGVEVSALIRRVQQEGGFATVLARGEAEAGTILLVLLE
                     RGAPARAVERMPMLDGTRAWQESAREDTDNTGKFTEWVERRHRQDPDLWVLELDIPSP
                     ERFIELSSPAG"
     gene            complement(11423..11926)
                     /locus_tag="VFF98_04740"
     CDS             complement(11423..11926)
                     /locus_tag="VFF98_04740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZU62969.1"
                     /translation="MAGPETAGHAAAPGAAGHWRALENLYATAPINRLFCSRLLIPGE
                     GVARIEFEVEPSCYHAAGAAHGTIYFKMLDDAAFYAANTLVTDRFLLTTSFNLHFSRP
                     VKAGPLVAEGRWISGRRRVLVGEARLIDAEGEEVGRGTGTFMRSQFALSGLSGYAETL
                     AGGTAGE"
     gene            complement(11928..12389)
                     /locus_tag="VFF98_04745"
     CDS             complement(11928..12389)
                     /locus_tag="VFF98_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZU62970.1"
                     /translation="MTGLFTLLPDAVGIIRADSKAAVVEQLAARFASVYGLDEALVRQ
                     RIEERERLGSTGFGRGVAIPHARIGDLPKPVAAFFRLAGPVAFESADDMPVDLVFGLL
                     SPEQAGAAHLHALAAISRLMRDEAMRLALLEAPGDEALYALLANVIDRDAA"
     gene            complement(12679..13242)
                     /gene="raiA"
                     /locus_tag="VFF98_04750"
     CDS             complement(12679..13242)
                     /gene="raiA"
                     /locus_tag="VFF98_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZU62971.1"
                     /translation="MDIRVSGHQMETGEALKAHASQRLSAIVDKHFNKALSSVVTFSK
                     APAGSFRCDIVTHVRQGLILKGAGLAHDAHQSLDQAAEKIDTQLRRYKRRLNDRHEQT
                     AHAIRAEDAAYTIFVEPEPEVEEHSDAPLVIAEMRVDVPEATVSDAVMMLDLRNTNAL
                     LFKNAGTGTYNMVYRRGDGSIGWVEPS"
     gene            complement(13273..13959)
                     /gene="dnaQ"
                     /locus_tag="VFF98_04755"
     CDS             complement(13273..13959)
                     /gene="dnaQ"
                     /locus_tag="VFF98_04755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZU62972.1"
                     /translation="MREIIFDTETTGLDPKTGDRLVEIGCIEMINRVVSGATFHAYFN
                     PGRDMPAEAERVHGLSEAFLADKPKFAERVAELLDFIGDSPLVAHNAGFDFGFLNAEL
                     DICGLPHVSRDRMVDTVAIARAKHPGAKNSLDALCTRYGIDRSHRTKHGALLDAELLA
                     QVYVELTGGRQIGLELVEDTVVEAVAAVSSLPEKQRPLRPARPHAASPEEIAAHAAFL
                     ETVKQPLWLE"
     gene            complement(14024..14623)
                     /gene="coaE"
                     /locus_tag="VFF98_04760"
     CDS             complement(14024..14623)
                     /gene="coaE"
                     /locus_tag="VFF98_04760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415511.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZU62973.1"
                     /translation="MTRPYVLGLTGSIGMGKSAVAAMLADLGAPVFDADATVRAMQGP
                     GGPLLPAIEAAFPGTTGADGVDRAALGAAVFGQPAALARLEGIVHPAVAAARAAFLAA
                     HGDAPLVVFDIPLLFEKGRAAEIDSVAVVSAPPALQRQRVLARPGMSAEKFAAILAHQ
                     MPDAEKRALADHVIDTGTSLAATQAAVAALYRQLARPVR"
     gene            complement(14623..15492)
                     /locus_tag="VFF98_04765"
     CDS             complement(14623..15492)
                     /locus_tag="VFF98_04765"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZU62974.1"
                     /translation="MSTDAQLAWHALPPLARACAEVIGDPIAQSKSPLIHRFWLARLG
                     LAADYRHAHVLAGDVPAYIAARRADPLWRGCNITMPHKQAVMPLLDRIDPLAARIGAV
                     NTVVRGEDGELSGFNTDAPGFMEPLAGLSGRALVLGAGGAARAIVLALADAGFALTLA
                     ARDAAKAAAVLAELAPGGPHQPAPLAAFAQGGAGAFDLVVNASPLGMVGQPALAFDLA
                     HLRPGGTVYDIVTAPAVTPLLAAARARGLATVDGLAMLIGQADYAFRRFYGARPDRAD
                     GDAALRALLAGAR"
     gene            complement(15489..16106)
                     /locus_tag="VFF98_04770"
     CDS             complement(15489..16106)
                     /locus_tag="VFF98_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZU62975.1"
                     /translation="MTVPDLTPAAPALWLASQSASRRAMLDNAGLAYTARPAAVDERA
                     LEAAMADAAPADVALALAEAKAQALSGLPAGDLVLGSDSLVVCDGRRFDKPASRADAA
                     SHLAFFSGKALALHSAAALVRDGAVIWRHADVARLTVRPLSPAFIEAYLDVEWPAVAG
                     CVGVFRMEGPGIQLFAQVDGHHFTILGMPLLPVLGALRQFGVLLA"
     gene            complement(16103..>16641)
                     /locus_tag="VFF98_04775"
     CDS             complement(16103..>16641)
                     /locus_tag="VFF98_04775"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HZU62976.1"
                     /translation="SKGRPGRQHTLDAAYFARVEAIQFTIAHDDGIAWEDWEEADIVL
                     AGVSRTSKTPTSIYLANRGFKVANIPIVVESPPPSALFGLKSPLVVGLTTAPERLIQV
                     RRNRLLTLNQSPDTAYVDMERVAREVQLARRMFADNGWPVIDVTRRSIEETAAAIINL
                     LKEHQVHPGLGQSGSKPI"
BASE COUNT         2785 a         5715 c         5531 g         2610 t
ORIGIN      
        1 cccgccggtc atcgtcaaca ccccaccgcg cagggcgcaa aagccttccg cccccgccgc
       61 atcgcgccag cgcagcaccg agggcggcag cacggtcaac agatcggcat ggcccggcaa
      121 caggccaaag ctgccgctct cgtccatcgc gcgcacggag cgcacctcgg cttccaccac
      181 cagcctttcg ggcgtggaga tggcaagggc gataaggccg ctcatgcagg cacgccctcg
      241 gcggcattgg ccgcctcttc cttggcgcgc gcctcggcca gcgtgccgat catgaacagc
      301 gagctttccc gccagctgtc gcattcgcca tcgagaatgg cgcggcagcc ggcgatggtg
      361 tcggccagcg cgacagagcg gccgggcgtg ccggtgaagg cctcggttac cgtgaagggc
      421 tgggtgagga agcgctgcag gcggcgcgcc cggccaacga tcagccgatc ctgcgcgccc
      481 agttcctcca tgcccagcag cgagatgaca tcctgcagct cgcggtaatg ttccagcgcg
      541 cggcgcacct cgatggcggt gcgggcatgt tcctcgccca ccacatcctt gtcgagcagc
      601 accgaggagg aagcgatggg atccaccgcc ggatacatcc cctcggccgc catggagcgc
      661 gaaaggacga tcatcgaatc gatatgggcg gcaatcgtcg tcaccgccgg atcggtgaaa
      721 tcatcggccg gcacataaac cgcctcgatc gcggtgatcg ccgcgccgcc ggcgggcgca
      781 atgcgttcct gcagcgcggc cacttcgctg gcaagcgtgg gctgatagcc aacgcgcgag
      841 ggcagccgcc ccagcaggcc cgacacttcc gcccccgcct gcacgaagcg gaagacatta
      901 tccatcagca gcagcacgtt cctgtgctgc gcatcgcgga aatattcggc gatggtcagc
      961 gccgtcatcg gcacgcgcca gcgggcgccg ggcggctcgt tcatctggcc atagaccagc
     1021 accgtgcgct cgatcacccc cgatccgcgc atgtcgagca gcatctcgtg cccctcgcgc
     1081 gagcgttcgc caacgccggc aaacaccgac aggccctgat aggaactgac catggcgtgg
     1141 atcagctcca tcaccagcac cgtcttgccc acgcccgccc cgccgaacat cgccgccttg
     1201 ccgccctggg ccagcggggt cagcaaatcg atcaccttga tgccggtgat gaaacgctcc
     1261 gtatcgccgg aggaaggagc cagcggcggc ggtgcgcggt ggatcggcca gcgcggggtg
     1321 tcctcggcca gcggcgcgcc gccatcgccc acatcgcccg acacggtgag cagccggccc
     1381 agcaccgcat cgcccaccgg cgccgtgagc gccgcgcccg tggccagcgc cggggtgccg
     1441 cgggcaaggc cggtggtggg ggcaagggca atggcgcgca ccgtgtgcgg atcgagatgg
     1501 gcctgcacct cggcaaggat ggcgccagcg ggaagcgcga tgcggatcgc ttcctccagc
     1561 agcggcaggc cgccctcgaa gcggatatca accaccgccc cgcgcacggc caccaccgtg
     1621 ccgcgtgctt cgccctgctc gctcacagcc tcggtcatag gtgctgtccg actcctgctg
     1681 gcggccatcg tcggcccgcg ctaagccttt gtcaatcatc ggtgaacctg cattgattca
     1741 ggtcatcgcg gccacgcccc tggcccatgc ccccttgccc ttgccggcat cttctgcttg
     1801 gcccggggca tggtcaggct gttccacatc tccgatctgc atttcggcgc cgaggatcgc
     1861 gccgcgcttg gctggtttgc ccaggtagtg gccgaggaaa agccctgcgg catcatctgc
     1921 accggcgatc ttaccatgcg cggcaccgtg cgcgaattca acgcggcggc cgattatctg
     1981 ggccggctgg cagcgcctgt gcatatcgag ccgggcaacc acgacatgcc ctattactgg
     2041 gaaatgggcg cccggctgcg ccacccgttc cgccgctttg gcgccctgcg ggcaaaggcc
     2101 caccgcgccc tgccgctggc cgatgtggcg gtggtgggcc tgtccaccgt ggtgccggcg
     2161 caatggcggc tcaactggtc gaaaggctgc gtgcgcgaaa ccgtgctggc caccgccctg
     2221 cacgaactgg cccacgccgg cccgcaggcg ctgaagctgg tggcctgcca ccacccctta
     2281 cgcgatgccg acacccatgg cagcggctcc acccggggcg gcggccgcgc gctggcgcag
     2341 ctggcggcgg ccggggccca tgcggtgctc tcgggccatg tgcatgatcc gttcgacatg
     2401 atgacccaaa cgccctgtgg ccccatccgc atgatcggcg ccggcaccct gtcggaacgc
     2461 acgcgcgcca gccgcccctc gttcaaccag ctggactggg atggcacgct gcgcgtgacc
     2521 gcgcgccacc acggctgatg caaaaagggc gggaaagcca aagccccccg cccctttcgc
     2581 agatcaaggc atcgccggcg gatcaggcca gcagatgctc cttggcgatc ttgtccttcc
     2641 acacggccgg ggccagctgg tggacattct tgccctcgga atcgaccgcg accgtgaccg
     2701 gcatgtcttc cacggtgaat tcatagatcg cttccatgcc gagatcggca aagccgacca
     2761 ccttgctggc cttgatggcg cgggccacca gataggcagc gccgcccacc gccatcagat
     2821 aggcgctctt gtggttggcg atggactggg tggcagcggg cccacgctct gccttgccga
     2881 cacaggccag caggccaagg ccgagcatcg tgtccatgaa cttgtccatg cgggtggcgg
     2941 tggtggggcc ggcagggcca accacctcgt cgcgcaccgg gtccaccggg ccgacatagt
     3001 agatcacccg gcccttgaac tcgaccggca gctcctcgcc cttggccagc atgtcggcga
     3061 tgcgcttgtg ggcagcgtcg cggccggtca acatcttgcc gttcagcagc aggcggtcac
     3121 cctgcttcca gctctgcact tcttccgccg tcagcgtgtc gaggttgacc ttcttggccg
     3181 ccgcatcggg cgcccagtgc accttaggcc attcatccag cttgggcgtt tcgagatacg
     3241 aaggcccgct gccatccagc gtgaaatggg cgtggcgggt ggcggcgcaa ttggggatca
     3301 tcgccaccgg cttgccggct gcatggcagg gcgcatccat gatcttcaca tcgaggatgg
     3361 tggcaaggcc gcccagccct tgcgcgccga tgcccagcgc attcaccttg tcgaagatct
     3421 cgatgcgcag ggcttccaga tcgttctggg ggccgcgctc tttcagctgg gccatatcga
     3481 tctgttccat cagcgccttc ttggccagca gcacgcaatg ctccgccgtg ccgccgatgc
     3541 cgatgcccag catgcccggc gggcaccagc cggcgcccat ctggggcagc atttccagca
     3601 cccaatcgac gatcgagtcg ctggggttca tcatcttgaa cttcgacttg ttctcgctgc
     3661 cgccgccctt ggccgccacg tccacgtgga ttttgctgcc cttcaccatg tccacatgca
     3721 gcacgcaagg ggtgttgtcc ttggtgttgc ggcgggtgaa ggcgggatcg gccagaaccg
     3781 aggcgcgcag cttgttctcg gggttgagat aggcgcggcg cacgccctca tccaccacgt
     3841 cctgaagcga cttttccgat tccaggcggc agttctggcc ccactggatg aagacgttga
     3901 cgatgccggt gtcctggcag atcggccggt ggccttcggc gcacatgcgg ctgttcgtca
     3961 ggatctgggc gatggcatcc ttggcggccg ggccctgttc ggcctcatag gcatcgccca
     4021 gcgcgcggat atagtccatc gggtggtaat agctgatgta ttggagcgca tcggcgacgc
     4081 tctcgatcag atcttcctcg cggatggtta ccatatcggc cattgcttgc gctcctcagg
     4141 gcgaccgggc ggcaaacggg gcttgcccgg caaggtagtg gggcgaccct ccagccgggc
     4201 cgcgggcgaa agacaggaag gcgcgccaga gccggcgtct gcctgtgtcg gcgcccccca
     4261 taggcgcgtt tgggcctgcc cgtcaaaagg ccaatgcacc gccatcgacc tttggatagg
     4321 atggcccgcg ccagaccccg cgcgtgccac gcaagccagc caccagaaag cggcacacac
     4381 gcgcgtcaaa acccttggct ttgcgccctt gcttggcata gaagcgcgcc aaagccgcgc
     4441 cgcaccagcc cggccccagc ggcaaaaggg actgaagcct atgaccacga ctgacaccgc
     4501 ccacgccagc ttcgacaccg cccgccgcgc catgatcgac agccagctgc gccccagcgg
     4561 cgtgaacgag ccctgggtgc tgaccgccat ggccgccacc ccgcgcgagg attatgtgcc
     4621 cgaaagtgcg cgcgcggccg cctatatcga tcgcgccctg ccgctgggcc agggccgctt
     4681 tctggcgccg gccgtggtgc agggccgcct gctgggcgaa gccgccccca cccaggccga
     4741 tcgcgtgctg gtgatcaccg gcggcaccgg ctatctcacc gcgctggtca gccagctggc
     4801 gcccaatgtg gatgcggtgg atgcggccga tctttccgcc attcccggca gcggctattc
     4861 gctgatcatc atcgacggcg ccatcgaagt gctgcccgat gccctggccg acaagctggc
     4921 cgagggcggg cgcattgcca ccggcctgct ggaaggcggc gtgacccgca ttgccaccgg
     4981 cgagaagctg gccggcaagg tggtgctggt ggcgctgtcg gatctgccgg tgccggtgct
     5041 gccggaattt gccgcgccca agcactggtc gttctgacca tgcgcgctgc ggcgcgcgta
     5101 tcctgccggc tggctattct ggcccttggc gcaagccccg gggccggcca tgcggaggat
     5161 ctgcgcgccg ccctggccgc cgcctatgcg gccaacccca cgctggcggc cgcgcgcgaa
     5221 accctgcgcg ccactgacga gggcgtggcc attgcccgcg ccgatggcct gccatctgcc
     5281 agcgccagcg tctcggaaac cgaaaatctg gtgcagagcc agctgagcct gggcgatgcg
     5341 ccgcgcgcgc tggcgctggc gggcacgctg tcggtgccgg tctattccgg cggcgcggtg
     5401 cgcaacggaa tgaaggcggc agaccggcgg gtgctggccg gccgcgccga tctggccgga
     5461 tcggaaagcg gcctgtttgc gcaagtggtg gccgcctaca tggatgtgcg gctgggcgag
     5521 gaaacgctgc ggctgaaccg cgccgaggtg gaaacgctgc ggcaaaccca gcgcgccact
     5581 gccgatcgct tccacgtggg cgaggtgacg aagaccgacc tggcccaggc ccaggcgcgg
     5641 ctggcgctgg ccgagggcag cctgcgcagc gccgaggcca atctggtgca ggcgcgcgag
     5701 acctatggcc agctggtggg ccacgcgcct gcagccctgg ccgatccgcc gccgctgccg
     5761 ggcctgccgg ccagcgtgga agaggccgag gctcatgccc tggccgacaa ccccgacctg
     5821 gccgccgccc gcgaacgcag ccgcgccgcc gcctatgaca cgcgcgccgc cggcgctgcc
     5881 cgcctgccga aggtgagcct gttcgccgat gccggctaca gcgattatct cggcgggctg
     5941 aaggtgctgg gctatccctc ggcggccttt ccgcaaagct tcacctctgc cgatgtgggg
     6001 gtgcgggcca ccattccgct ctatcagggc gggcggcccg gcgcccagat ccgccaggca
     6061 gaagcgcgcg aaggcgccgc gcaagacaat gagatcgccg ccgaacggca ggtgatcgcc
     6121 cagctgcgca gcgcctatgc cagctggcag gccgcccgcg ccatcatcgc ctataatgaa
     6181 accgccgtgg ccgccgcccg gctggggctg cagggcgtgc gcgcggaaaa tggcgtgggc
     6241 aaccgcaccg tgctcgacat cctcaacgcc gagcaggaac tggtaagcgc gcaagtgcag
     6301 ctggccaccg cccggcgcaa tgcctatgtc gccggcttca gcgtgctggc ggcgatgggc
     6361 cgggccggcg cgcgcgatct ggggctggat ggcggcgcgc tgtacgatcc ggcgctgcat
     6421 tatcgccaga tgcgccggtc cctcggcgat gcgccgggcc atcagggcgc ctctgccccg
     6481 ccgcagcccg ccagcacccc tgcgcgcaca accgttgaca cgccccccca aacagggaca
     6541 atcgagggtg ggtgatgtga ctctctttta gggattcgcc gataagcaga agcggagtga
     6601 tcgtgtatcg cctccaggag aattgggcca tgcgccagcc aggtgaacca gcggtggaag
     6661 accttcttga atcgatcaag aaggtcatcg cgcgcgataa tcaggtgacc gggcaggcag
     6721 gcgcacgggc aggcggagcc gagcagcgcc gcctgggcag cagcgccggc cttgccgccg
     6781 cgcatgatct gcgcggcttg gcgcaggccg cccctgccac cgcctttgcc gacgacgtgc
     6841 tggatctggc catgtctgcc cagttcctcg acgaccatca cgaggatgac atgcacaatt
     6901 acgggcacga caatcatggg cacgacgatg gcgatgagga agacaaggcg ctgatcgccc
     6961 ccgaggcgcg cgcctcgatg cgcgaatcgc tggcggcgct ggccatgctg gcccagcccg
     7021 gcaaggcccc gcagatcgtg cgcagcggcg aaacctcgct ggaggagctg acccgcgagc
     7081 tgctgcgccc ggcgctggcc caatggctgg atgaacacct gcccgcgctg gtagaaagca
     7141 tggtggccgc cgagatcgcc cgcatcgttg gcaagaaggc ctgagccccc gctgaaaggc
     7201 cagctgccgg cccgggcagg ctttgccatg ggcggcagac caccttccgg aaagccccct
     7261 tgccatggcc aaacccttgc cccaccagct ggatagccag cgctatccgc accgccatct
     7321 ggtgcccacg cgcttcagcg atctggatcc caacaaccat atcaacaatg tggcgctggc
     7381 cggcatcatc gaggatggcc gggtgcggct gcaccgcgat gccggctggt atgccccgga
     7441 gctgggggtg cagatcatgc tcgccagcct gacggtggaa tatctggcgc aatcgcacta
     7501 tccgctggat atcaccgtgc tttcgggcgt ggggcgggtg ggccgctcca gcctcgatgt
     7561 ggagcagctg gtgatgcagg aagggctttg cgtggcctat gcccgctctg tggtggtgtt
     7621 cgtggaggac ggggcggcag tgcccctgcc cggggacgtg ctggcccggc tggacagatg
     7681 gaagctgacg caatgaccgc aaggcaggag atcacccgcg ccgaagacgc gctggccaag
     7741 ctggcgcccg gccatgacgg gcccgtgcgc cacatcttcc tgtgcgccga gccgcagaag
     7801 ggcgaatgct gcacgcctga tgtgggcagc gcggcctggg cttacctcaa gaagcgctgc
     7861 aagcagctgg ggctggccga ggccggcatc caccgctcca aggcggattg cctgaagctg
     7921 tgcgcggcag ggccggtggc ggtcgtctgg ccggaaggcg tgtggtatca cagctgcacc
     7981 gcgccggtgc tggaacgcat tttgcaggag catctgatcg gcggcgtgcc ggtggcggaa
     8041 tatcgcctgc acgccccctg caaggcgggc agctaggcgt ccctgtcctc gtcgctgtcc
     8101 ctgttctcgt ccttgtcctc gtccccgggc tcggccgggg tgcccacggc gccatcatgg
     8161 cccgtgccgg cagacaggaa caccagcccc atcagccccg cggtgagcag catggcaaag
     8221 gaaatgccca ccgccaccgc gatatagaaa tgaacggaga ccagccccac ctgcttgtag
     8281 agcagcgcca gggcaatcag caccgtgccg gtggtgacca ggaacatcag ccgcatcagc
     8341 cgccgaaacc gcgcccaggc atgggccgcc accgccggat tatccagcgg ggaagggcgc
     8401 gccttgggcg ggggcagggg atctggccgg ggcatgtgct tcagatgcgc gccgcagcgc
     8461 cgcttggcaa gcgccggttt tctgcgctct ggcgcgggcg ggcggggcgt gtcatacttc
     8521 gtgtccacaa gataatcagg agagttgcca tgacgattgc ccgtctgctg gagcgccgta
     8581 cctcgcctgt cattttttgc agtattcaca cgcctgtacg cgaagcggtg acgcttctgg
     8641 cagagcggcg aatcggcgcc gtgccggtaa tggatggcga tgctgtggcc ggcatctttt
     8701 ccgaacgcga tgtgatctat caactgggcg cgcgcgggcc gggcatgctc gatctcaacc
     8761 tggggcaggt gatgacctcg ccggcgatca ccgtcagccc ggataccggc gtgctggaag
     8821 cgctgagcct gatcacccgc cggcgcatcc gccatctgcc ggtgctgcgc ggcgggcgca
     8881 tggtgggctt catctccatc ggcgatctgg tgaaataccg catcgacctg atcgaggccg
     8941 aagccgaagc catgcgcacc tatatccaga cggcctgacc cgtccaccag gccggctgga
     9001 agggcgcgcc ggcaccttgc gatagcgggt cggcgcgcct acattcagag gcatgagcga
     9061 aatggctatc accctatctc cttccgccgc cgcgcgcgtg gcgtggatcg ccgaaaagca
     9121 gggccaaccg gccattctgc gactggccgt ggaaggcggc ggctgctccg gcttccaata
     9181 tcgcttcggc ctggcagaag ccgtggccga ggacgatacc gtgagcgaaa ccgatggcgt
     9241 gaagctggtg gtagatgcgg tcagcctcga tctggtggcg ggctgcgtgg tggattatgt
     9301 ggaaagcctt ggcggcgccg ctttccgcgt ggaaaacccc caggccactg ccggttgcgg
     9361 ttgcggatcg agcttcgctg tctaaaccgg ctggcatgaa aatcgccagc ttcaacatca
     9421 acgggatcaa ggcccgtttg ccccgcctgc tggaatggct ggcggaaacc cgccccgccg
     9481 ttgcctgcct gcaggaaatc aaaaccgccg acgagggctt tcccgccgag gaattcgaga
     9541 agatcggcta tcaggcgatc tggcacgggc agaagggctt caacggcgtg gctatcctgg
     9601 ccgatggcgt gcagcctgtg gaagtgcgcc gcgggctggc gggcgagccc gaagacgagc
     9661 aatcgcgcta tctggaggcc gatgtgtttg gcttgcgggt ggcgggcatc tatctgccca
     9721 atggcaaccc gcagcccggc cccaagttcg attacaagct gcgctggatg gagcggctgc
     9781 gcgcccgcat ggccgagatt gccgccgagg aagtgccggc gatcgttacc ggcgatttca
     9841 acgttatccc gcgcgattgc gacatctgga gccccaaggc catggccgat gacgcgctga
     9901 tgcagccgca atcacgcgat gcctatgcca cgctgatgca tgatggctgg acagatgcca
     9961 tcgccacgct gaaccctggc ggcggcgtgt ggacctattg ggactatcag gcgggcgcct
    10021 ggcagaagga ccacggcttt cgcatcgacc atgcgctgct ctcgcccgaa ctggcggaca
    10081 ggctggtggc cgccggcgtg gacaaagccc atcgcgggcg cgaaaaggcc agcgaccatg
    10141 cgccgatctg ggtggaactg cgcgactgac gcccagcgct ggcgccgtcc acgaaaaagc
    10201 cgccaacggg aagccccgcg gcggcttttt tcataggtgc ccctgttctc gccggatcag
    10261 cgatagaaca cgtgattgtc cacccgggcg atccgctcgc ggtgccaggg gggggaaacg
    10321 cggcgggcat ggaaatacag cgcgccttcc accgggctat cccagctgcc cttgtcggca
    10381 atggcggcga tggccgcggc attgcgccag gcgtggctgg catggctggt ttccggcagg
    10441 ttgccgttgt gcacgaagga gaactggccg tgctggaaca ccacgccgca atagctggcg
    10501 gggaaacggc cggaatgcga gcgggcgacg atcacacgcg ccacggccag ctggccgctc
    10561 agcgtttcgt tgcgcgcttc gtagtagacg gcgctggcaa ggcagttcag ttccttgggc
    10621 agggcatctg cctgcggagc ctcggcaacg aggctggcca gatcctcgga agcgctggca
    10681 tcgtcatgcg cttcgggctg gcccgaaagg gtttgctcct gcgcggcgga ggtggctccg
    10741 gcctggggca gcaggttcac aggttccgct tgggccagcg cagtggcatt tttctgcgcg
    10801 gcggcgcccg aaccagcggc gccaaaagcg atcacggcga gggttgcggc cagggtgatc
    10861 aggctggcct tgttgaagat gaaacgcatg aatcgtcttg ttcaggcggt ggacaagccc
    10921 gccgcaggag gggctggccg aaacatgcga tccccgctgt ttccgctcgc cacaaatcct
    10981 gccggccgtc ccccgtctgc gcgcttgcct cgccggctgg aatgcctgac taagccgccc
    11041 acgcaaagat ggagccggct ctctgacggc aggggcccct cgagtcaacc cgccggggat
    11101 gacaattcga tgaaacgttc agggcttggg atgtccagtt ccagcaccca cagatccggg
    11161 tcctggcggt ggcggcgctc cacccactcc gtaaatttac ctgtattatc cgtatcttcc
    11221 cttgccgatt cctgccaggc gcgggtgcca tccagcatcg gcatgcgctc caccgcacgg
    11281 gcgggcgcgc ccctttccag cagcaccagc aggatcgtgc cggcctctgc ctcgcccctg
    11341 gccagcacgg tggcaaagcc gccttcctgc tgcacccggc ggatcagggc agatacttcc
    11401 acccccgcag gcagcctggc gctcattccc cggcggtgcc gccagccagc gtttcggcat
    11461 agcccgaaag gcccgacagg gcgaattgcg agcgcatgaa ggtgcccgtg ccgcggccca
    11521 cttcctcgcc ctcggcatcg atcagccggg cctcgcccac cagcacccgg cgccggccgc
    11581 tgatccagcg cccttcggcc accagcggcc cggccttgac cggccggctg aaatggaggt
    11641 tgaacgaggt cgtcagcagg aaccgatcag tgaccagcgt gttggccgca tagaaagcgg
    11701 catcatccag catcttgaaa tagatcgtgc catgggcggc accggcggca tgatagcacg
    11761 aaggctccac ctcgaactcg atgcgtgcca ccccttcgcc agggatcagc agacgcgagc
    11821 agaacagccg gttgatcggc gccgtggcat agaggttttc cagcgcccgc cagtggccgg
    11881 cagcgccagg cgcagccgcg tgcccggccg tctcaggccc agccatctca ggccgcgtcc
    11941 cggtcgatca cattggcgag cagggcataa agcgcctcgt cgcccggcgc ttccagcagg
    12001 gccaggcgca tggcttcatc gcgcatcagc cgcgagatcg ccgccagcgc atgaagatgg
    12061 gccgcgcccg cctgttcggg cgagagcagg ccaaagacca gatccaccgg catatcgtcg
    12121 gccgattcga aagccaccgg gcctgccagg cggaagaagg ccgccaccgg cttgggcaga
    12181 tcgccgatgc gcgcgtgggg aatggccacg ccccggccaa agccggtgct gcccagtctc
    12241 tcgcgctctt cgatccgctg gcgcaccagc gcttcgtcta gcccatagac cgaggcaaac
    12301 cgcgccgcca actgctcgac gaccgccgct ttgctatcgg cccggataat cccgaccgca
    12361 tcgggaagga gggtgaacaa cccagtcatc gccaaatcgc aacaagcctt ttcttcgcgc
    12421 ggaggacggc gcccgaataa ttgggcacgg cccgctgccg gttatccatt ttgccgcgcc
    12481 gcgccagccc aaaactgccg caagcgtgca aattttcctc ctggcgaatg acctggtcct
    12541 tggaacagac cgctttatct ggccactata ttcgggtttt cactgtatca ccgcaaaccc
    12601 gccttgccaa acaaaccgca aggccgggcg ccgattggcg ggtgatgttc aacccgccaa
    12661 ccgatctggc agcctggctc agctgggttc aacccagccg atcgagccat cgccgcggcg
    12721 gtaaaccata ttataggtgc ccgttccagc gtttttgaac agcaaggcgt tggtgttcct
    12781 gaggtccagc atcatcaccg cgtcagagac ggtggcctcg ggcacgtcca cgcgcatttc
    12841 ggcgatcacc agcggcgcat cggaatgctc ctccacttcc ggttcgggct cgacgaagat
    12901 ggtataggcc gcatcttcgg cgcggatggc gtgagcggtc tgctcgtggc ggtcgttcag
    12961 ccggcgcttg tagcggcgca gctgggtgtc gatcttctcg gcggcctggt cgaggctctg
    13021 atgggcatca tgggcaagcc cggcgccctt caggatcagc ccctggcgca catgggtcac
    13081 gatatcgcag cggaaggagc cggccggcgc cttgctgaac gtgaccaccg aggacagcgc
    13141 cttgttgaaa tgcttgtcga caatcgcgga caaccgctgc gaggcatgag ccttgagcgc
    13201 ctcaccggtt tccatctggt ggcccgaaac gcgaatgtcc atagcgctac tccttctgac
    13261 ttatgttctt cgtcactcca gccacagggg ctgtttcacc gtttcgagga aggcggcatg
    13321 ggccgcgatt tcctccgggc tggccgcatg ggggcgggca gggcgcaagg gccgctgctt
    13381 ttccggcagg ctggacacgg ccgcaaccgc ctccaccacc gtatcctcca ccagttccag
    13441 accgatctgc cggccgccgg tgagttcgac atacacctgg gccagaagtt cagcatcaag
    13501 cagcgcgccg tgcttggttc tatgactgcg atcaataccg tagcgagtgc acagggcatc
    13561 gagcgaattc ttcgcgccgg ggtgcttggc gcgcgcgatc gccacggtgt cgaccatgcg
    13621 gtcgcggctg acatggggca ggccgcagat atccagctcg gcattgagga agccgaaatc
    13681 gaagccggca ttatgcgcca ccagcggcga atcgccgatg aaatcgagca gctcggccac
    13741 cctttcggca aacttcggct tgtcggcgag gaaggcttcc gacaggccat gcacccgctc
    13801 ggcctcggca ggcatatcgc gcccggggtt gaaataggca tggaaggtcg cgccgctgac
    13861 gacccggttg atcatctcga tgcaaccgat ttccacgagc cgatcccccg ttttcggatc
    13921 gaggccggtg gtttcggtgt cgaagatgat ctcacgcatt tgcatggtta ttgacctgtg
    13981 cgcggccggt tacaagccgg attctggcac tagggagatg tgcttatcgc acggggcgcg
    14041 ccagctgccg gtaaagcgcg gcaacggccg cctgcgtggc cgcaaggctg gtgccggtgt
    14101 cgatcacatg atcggccagc gcgcgttttt cggcatcggg catctggtgg gcaaggatgg
    14161 cggcgaattt ctcggcgctc atgcccgggc gggccagcac gcgctggcgc tgcagggcgg
    14221 gcggggcaga aactaccgct accgagtcga tttccgccgc gcggcccttc tcgaacaaca
    14281 gggggatatc gaagaccacc agcggcgcat cgccatgcgc ggcaaggaac gccgcccgcg
    14341 ccgccgccac ggccgggtgc acaatgccct ccagccgggc cagtgccgca ggctggccga
    14401 acaccgccgc gcccagcgct gcgcggtcca ccccatcagc gccggtggtg ccggggaagg
    14461 cggcctctat cgccggcagc agcgggccgc ccgggccctg catggcgcgc acggtggcat
    14521 cggcatcgaa caccggcgcg cccagatcgg ccagcatcgc tgccaccgcc gatttgccca
    14581 tgccgatgga gccggtgagc cccagcacat aggggcgcgt catcaccgtg cgcctgccag
    14641 cagcgcgcgc aaggcggcat cgccatcggc gcgatcgggc ctggcgccat agaagcggcg
    14701 gaaagcataa tctgcctggc cgatcagcat cgccagccca tccaccgtgg ccagcccgcg
    14761 cgcccgcgcc gcggccagca agggcgtgac cgccggcgcg gtgacgatat catagaccgt
    14821 gccaccgggg cgcaaatggg ccagatcgaa ggccagcgcg ggctggccca ccatgcccag
    14881 cgggctggca ttgaccacca gatcgaacgc ccccgcgccg ccttgcgcaa aagcggccag
    14941 gggcgctggc tgatgcggcc caccgggcgc cagttcggcc agcaccgcgg ccgccttggc
    15001 ggcatcgcgc gcggcaaggg tgagggcaaa gccggcgtcg gccagggcca gcacgatcgc
    15061 ccgcgcagcc ccgcctgcgc ccagcaccag cgcccggccg gaaaggccgg ccagcggttc
    15121 catgaagccc ggcgcatcgg tgttgaagcc cgagagctcc ccatcctcgc cgcgcaccac
    15181 cgtgttcacc gcgccgatgc gggcggccag cgggtctatc ctgtcaagca ggggcatcac
    15241 cgcctgcttg tgcggcatgg tgatattgca gccccgccac agcggatccg cccgccgcgc
    15301 ggcgatatag gccggcacat cgcccgccag cacatgggcg tggcgataat ccgccgccag
    15361 gcccagccgg gccagccaga agcgatgaat gagcggcgat ttcgactggg cgatgggatc
    15421 gccgatcact tcggcacagg cgcgcgccag cggcggcagg gcgtgccagg ccagctgggc
    15481 atccgtgctc atgccagcag caccccgaac tggcgcagcg cccccagcac cggcagcagc
    15541 ggcatgccca gaatcgtgaa gtgatggcca tcgacctgcg cgaacagctg gatgcccggc
    15601 ccttccatgc gaaacacgcc cacgcagccg gccaccgccg gccattcgac atcgagatag
    15661 gcctcgatga aagccgggct cagcgggcgc acggtcaacc gggccacatc ggcatggcgc
    15721 cagatcacgg cgccatcgcg caccagcgcg gcggcggaat gcagcgccag cgccttgccg
    15781 gaaaagaagg cgagatggct ggcggcatcg gcgcggctgg ccggcttgtc gaaccggcgg
    15841 ccatcgcaca ccaccagcga atcgctgccc agcaccagat cgcccgccgg caggcccgac
    15901 agcgcctgcg ccttcgcctc ggccagcgcc agcgccacat cggcaggcgc tgcatcggcc
    15961 atcgccgctt ccagcgcgcg ctcgtccacc gcggcggggc gggcggtgta ggccaggccc
    16021 gcattatcca gcatggcccg gcgcgaggcg ctttgcgagg ccagccacag ggcaggggcg
    16081 gcaggggtca ggtcgggcac ggtcaaatcg gcttgcttcc gctttgccca aggccaggat
    16141 gcacctgatg ctccttgagc aggttgatga tcgccgctgc cgtctcctcg atcgagcggc
    16201 gggtgacatc gatcaccggc cagccattat cggcaaacat gcggcgggca agctgcactt
    16261 cgcgggccac ccgctccata tccacatagg cggtatcggg cgactggttg agcgtgagca
    16321 ggcggttgcg gcgcacctgg atcagccgct cgggcgcggt ggtcagcccc accaccagcg
    16381 gcgatttgag gccgaacagg gccgaaggcg gcgggctttc caccacgatg gggatattgg
    16441 ccaccttgaa gccgcgattg gccagataga tgctggtggg cgttttcgac gtgcgcgaca
    16501 cgcctgccag cacgatatcg gcttcctccc aatcttccca ggcaatgcca tcatcatggg
    16561 cgatggtgaa ctggatcgcc tccacccggg cgaaataggc cgcatccagc gtgtgctggc
    16621 ggcccggccg gcccttggat t
//
LOCUS       DASRYE010000085         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_111513,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000085 DASRYE010000000
VERSION     DASRYE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111513"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2664
                     /locus_tag="VFF98_04780"
     CDS             <1..2664
                     /locus_tag="VFF98_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU62977.1"
                     /translation="GKAALGEEELTRRRLVQDWTVRPLLRSISGVAEINSQGGFARQY
                     QILVNPDRLRHYEVSVADVFQAVARNNANAGGGVLPQMAEQYLIRGQGLVKSEEDLRA
                     IVIRESGNQPVYVRDVAEVKIGPGVREGAVIKDGATESVGGVVMMMAGGNAKQVVSRI
                     KEKVDEINARGMLPDGLQIKPYYDRSELVDAALHTVTKVLMEGIVLVVVVLLLFLGDI
                     RSSLIVVATLVLTPLLTFLCMNRLGISANLMSLGGLAIAIGLMVDGSVVVVENAFARL
                     GEAAHSGESRVRIILSAVVEVATPVIFGVGIIILVFLPLMTLEGMEGKMFAPLAYTIA
                     IALAISLALSLTLTPVLSSYLLKAPAHGGDHDTRLIAWMKRRYLAMLGWALGHERKTV
                     LYALGILAGTLCLFPLLGTAFIPEMKEGSLVPGINRVPNISLDESIRIEMEAMKLVMQ
                     VPGVKSAVSGVGRGESPADPQGPNESTPIVSLKPRAEWPKGWNQDDIADAMREKLKAI
                     PGIQIVMAQPISDRVDEMVTGVRSDIAVKVFGDDLGQLKATAEQIARVAGGMKGAEDL
                     RIEKISGQQYLSIDIDRQALARYGLNVADVNDVIETAIGGKAATEIFEGERRFPAVVR
                     LPAEYRGNIQAIADLLVFAPGGAQVRLADIAHIAVEDGPAQISREHARRRIVVGMNVK
                     GRDLGGFVAELQKKVGAEVKLPEGYVLEWGGQFENMQRAMGHLMIIVPITVAAIFFLL
                     FVLFNSLRLATLIITVLPFASIGGVIGLFVSGEYLSVPASVGFIALWGIAVLNGVVLV
                     SYIRKLREEGLSVREAVEKGATARFRPVMMTATVALLGLIPFLFSTGPGAEVQRPLAI
                     VVIGGLITSTLLTLLVLPTIYRFFDSEPLEA"
     gene            2713..3042
                     /locus_tag="VFF98_04785"
     CDS             2713..3042
                     /locus_tag="VFF98_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZU62978.1"
                     /translation="MIEIRAIIRPARLETLRQALRQMPEFPGMTVLKVEGCSAPWVEQ
                     AEPAGIRRELLDYTPKAMVIMVAPEDAAERLCATIHRVAHTGRMGDGLIWTGEVRHFM
                     RIATQPE"
     gene            complement(3083..3832)
                     /locus_tag="VFF98_04790"
     CDS             complement(3083..3832)
                     /locus_tag="VFF98_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU62979.1"
                     /translation="MTIRCQQQGAVRLLTIDRPAASNALDAATSAALDAAITAAEADE
                     ATGAVVLTGAGPRAFCAGMDLKEAARIGAGHGLIAGRGFGGITERQRTKPLIAAVNGA
                     AVAGGFEIVLACDIVVAAEGAPMGLAEVKHGLFAFAGGIQRLARQVPRATALAMILTG
                     ELVPARRLYDLGVVSEVVPAGEVLPRALAIATAMLAHSWPAIANGKLLYELACGMETG
                     QALAMGRAWGMATLERAGAPLPGAPDPAEPR"
     gene            complement(3829..4383)
                     /locus_tag="VFF98_04795"
     CDS             complement(3829..4383)
                     /locus_tag="VFF98_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU62980.1"
                     /translation="MADLEARITRLEAESQIRQLIAHYCFDIDDRHIAAIGRLFTADG
                     VLRSADGVMYAEGPEAIIAQYHRRFDVLGPGHHFMHDVQIDFVGDGTQEATGRVSGHA
                     ELMRKGQMMIAALRYADRYRRTAEGWKFAERVISFLYYVPVEDYPGILLTPDRNRAYD
                     RPMPADFPEPLASWQAYHQNGRRP"
     gene            4602..5186
                     /locus_tag="VFF98_04800"
     CDS             4602..5186
                     /locus_tag="VFF98_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU62981.1"
                     /translation="MPKKVDHARRRLDIARVAEGLVYDKGMDGLTMRDVARGMGCSLS
                     VILHYFESKLDLLVFTHRLVRHRAEAGLVKALEEGQPARACFEHLLPLDEQRWRDWYT
                     WLAFWGMSSQAGEVASEHVAATVESADIFLRIIRHGQARGEIDTAFDAAALAIDMQIL
                     CNGLAGLVAQGREAWPAERQLAHFRRHYAALIRR"
BASE COUNT          915 a         1816 c         1925 g          962 t
ORIGIN      
        1 ggcaaggccg cgcttggcga ggaagagctg acccgccgcc gcctggtgca ggactggacc
       61 gtgcgcccgc tgctgcgctc catctcgggc gtggccgaga tcaactcgca gggcggcttt
      121 gcccggcaat atcagatcct cgtcaacccc gaccggctgc gccactatga ggtgagcgtg
      181 gccgatgtgt tccaggcggt ggcgcgcaac aatgccaatg ccggcggcgg cgtgctgccg
      241 cagatggccg agcaatatct catccgcggc caggggctgg tgaaaagcga ggaggacctg
      301 cgcgccatcg tcatccgcga aagcggcaac cagccggtct atgtgcgcga tgtcgccgag
      361 gtgaagattg gccccggcgt gcgcgagggc gcggtgatca aggatggcgc cacggaaagc
      421 gtgggcggcg tggtgatgat gatggccggc ggcaatgcca agcaggtcgt cagccgcatc
      481 aaggagaagg tggacgagat caacgccagg ggcatgctgc ccgatggcct gcagatcaaa
      541 ccctattacg accgttccga gctggtcgat gccgcgctcc acaccgttac caaggtgctg
      601 atggaaggca ttgtgctggt ggtggtggtg ttgctgctgt tcctcggcga tatccgctcc
      661 tcgctgattg tggtggcaac gctggtgctc acgccgctgc tcaccttcct gtgcatgaac
      721 cggttgggca tatcggccaa tctgatgagc cttggcggcc ttgccattgc cattggcctc
      781 atggtcgatg gatcggtggt ggtggtggaa aacgcctttg cccggctggg cgaggcggcc
      841 cacagcggcg agagccgggt gcgtatcatc ctgtcggcgg tggtggaagt ggcaacgccg
      901 gtgatctttg gcgtgggcat catcatcctc gtcttcctgc cgctgatgac gctggagggg
      961 atggagggca agatgtttgc cccgctggcc tataccatcg ccattgccct ggcgatttcg
     1021 ctggcgctca gcctcacgct cacgccggtg ctctcgtcct atctgctgaa ggcgccggcc
     1081 catggcggcg atcatgacac gcggctgatc gcctggatga agcggcgcta tctggcgatg
     1141 ctgggctggg cgctgggcca tgaacgcaag acggtgctgt atgcgctggg cattctggcc
     1201 ggcacgctgt gcctcttccc gctgctgggc acggccttca tccccgagat gaaggagggc
     1261 tcgctggtgc cgggcatcaa ccgggtgccg aatatctcgc tcgatgaatc gatccgcatc
     1321 gagatggagg cgatgaagct ggtcatgcag gtgcccggcg tcaaatcggc ggtgtcgggc
     1381 gtggggcggg gcgaaagccc ggccgatccg caagggccca atgaatcgac ccccatcgtc
     1441 agcctgaagc cccgcgccga atggcccaag ggctggaacc aggacgatat cgccgatgcc
     1501 atgcgcgaga agctgaaggc cattcccggc atccagatcg tcatggccca gcccatttcc
     1561 gaccgcgtgg acgagatggt gaccggcgtg cgctcggata tcgccgtgaa ggtgtttggc
     1621 gatgatctgg gccagctgaa ggccaccgcc gagcagatcg cccgcgtggc cggcggcatg
     1681 aagggcgccg aggatctgcg catcgagaag atcagcggcc agcaatatct ctcgatcgac
     1741 atcgatcggc aggcgctggc ccgctatggc ctcaacgtgg ccgatgtgaa cgatgtgatc
     1801 gagacggcga tcggcggcaa ggcggccacc gagatcttcg agggcgagcg gcggttcccc
     1861 gccgtggtgc gcctgccggc cgaatatcgc ggcaatatcc aggcgattgc cgatctgctg
     1921 gtgtttgccc ccggcggcgc gcaggtgcgc ctggccgata tcgcccatat cgccgtggag
     1981 gatggccccg cccagatcag ccgcgaacat gcccgccgcc gcatcgttgt gggcatgaat
     2041 gtgaaggggc gcgatcttgg cggctttgtt gccgagctgc agaagaaggt gggcgcggag
     2101 gtgaagctgc ccgaaggcta tgtgctggaa tggggcggcc agttcgagaa catgcagcgc
     2161 gccatgggcc atctgatgat catcgtgccg atcaccgtgg cggcgatctt cttcctgctg
     2221 ttcgtgctgt tcaattcgct gcggctggcc acgctgatca tcaccgtgct gccgtttgcc
     2281 tccattggcg gggtgatcgg cctgttcgtc tcgggcgaat atctctcggt gccggcatcg
     2341 gtgggcttta tcgcgctgtg gggcattgcg gtgctgaacg gggtggtgct ggtctcctat
     2401 atccgcaagc tgcgcgagga gggcctctcg gtgcgcgagg cggtggagaa gggcgccacg
     2461 gcccgtttcc gcccggtgat gatgaccgcc accgtggcgc tgctggggct tatccccttc
     2521 ctcttctcca cagggccggg ggccgaggtg cagcggccgc tggcgatcgt ggtgatcggc
     2581 gggctgatca cttccaccct gctcaccctg ctggtgctgc ccaccatcta ccgcttcttc
     2641 gacagcgaac cgctggaagc ctgaccggtg cttcatttct tacagggccc catttcccac
     2701 agggagagac acatgatcga gatccgggcg attatccgcc ccgccaggct ggaaaccctg
     2761 cgccaggcac tgcgccagat gccggaattt ccgggcatga cggtgctgaa ggtggagggc
     2821 tgcagcgccc cctgggtgga gcaggcagag ccggccggca tccgccgcga actgctggat
     2881 tataccccca aggccatggt catcatggtg gcgccggaag atgccgcaga gcgcttgtgc
     2941 gccaccatcc accgcgtggc ccacaccggc cgcatgggcg acgggctgat ctggaccggc
     3001 gaggtgcgcc atttcatgcg catcgccacc cagccggagt aaactggggc ccgaatgagc
     3061 cgggggccgg agtgagccgg gatcagcgcg gttcggcggg atcaggggcg ccgggaaggg
     3121 gcgccccggc ccgctccagc gtggccatgc cccaggccct gcccatggcc agcgcctggc
     3181 cggtttccat gccgcaggcc agctcataca gcagcttgcc attggcgatc gccggccagc
     3241 tatgggccag catcgctgtg gcgatcgcca gcgcgcgggg cagcacctcg cccgccggca
     3301 ccacttcgct gaccacgccc agatcataga ggcgccgcgc cggcaccagc tcgccggtca
     3361 ggatcatggc aagggcggtg gcgcgcggca cctgccttgc cagccgctgg atgccgccgg
     3421 caaaggcgaa caggccgtgt ttcacttccg ccaggcccat cggcgcgcct tctgcggcca
     3481 caacgatatc gcaggccagc acgatctcga agccgcccgc caccgccgcc ccgttcaccg
     3541 cggcgatcag cggcttggtg cgctgccgct cggtgatgcc gccaaagccg cgcccggcaa
     3601 tcagcccgtg gccggcgccg atgcgggcgg cctccttcag gtccatgccg gcgcaaaagg
     3661 cgcgcgggcc cgcgcccgtc aacaccacgg cgccagtggc ttcatccgcc tcggcggcgg
     3721 tgatcgctgc atcaagcgcg gccgaagtgg cggcatcgag cgcattgctg gcggccggcc
     3781 ggtcgatcgt cagcagccgc accgcgcctt gctgttggca gcggatggtc atggccggcg
     3841 cccgttctgg tgataggcct gccagctggc cagtggctcg gggaaatcgg ccggcatcgg
     3901 ccggtcatag gcgcggttgc gatcgggcgt gaggagaatg ccgggatagt cctccaccgg
     3961 cacgtaatag aggaaagaga tcacccgctc ggcgaatttc cagccctcgg ccgtgcggcg
     4021 atagcgatcg gcatagcgca gcgcggcgat catcatctgc cccttgcgca tcagctcggc
     4081 atggccggaa acgcgccccg tggcctcctg cgtgccatcg ccgacaaaat cgatctgcac
     4141 atcatgcatg aagtggtggc ccggccccag cacgtcgaag cggcggtgat attgggcgat
     4201 gatcgcctcg ggcccctctg catacatcac cccatcggcc gagcgcagca cgccatctgc
     4261 ggtgaacagc cggccgatcg cggcgatatg gcgatcgtcg atatcgaagc agtaatgggc
     4321 gatcagctgg cggatctgcg attccgcttc cagccgggtg atgcgcgctt ccagatcggc
     4381 catgctctct cttctcgttc cggcgccggt tggcggggag cataaccggc ccggtttaca
     4441 aaacaaacag aaatgttttt aatagggtgc cgccggtggc cgcctcgcca aatggcgagg
     4501 gcgcgcgtgc gcggcctttc ccgccggggc caagggccgc tatgctggcc cgcttcactg
     4561 ccccgcttca tcgcccatgc aacagacgag acactgccac catgcccaag aaggtcgatc
     4621 atgcccggcg ccgcctcgat atcgcgcggg tggccgaagg gctggtctat gacaagggga
     4681 tggatgggct gaccatgcgt gatgtggccc gcggcatggg ctgcagcctg tcggtcatcc
     4741 tccattattt cgaaagcaag ctcgatctgc tggtcttcac ccaccggctg gtgcgccacc
     4801 gcgccgaggc gggcctggtg aaggcgctgg aagagggcca gccggcgcgc gcctgtttcg
     4861 agcacctgct gccgctggac gagcagcgct ggcgcgactg gtacacctgg ctggccttct
     4921 ggggcatgtc ctcgcaggcg ggcgaggtgg ccagcgaaca tgtggccgcc acggtggaat
     4981 cggcggatat cttcctgcgc atcatccgcc acggccaggc ccggggcgag atcgacaccg
     5041 ccttcgatgc cgccgccctg gcgatcgaca tgcagatcct gtgcaatggc cttgccggcc
     5101 ttgtggccca gggccgcgaa gcctggccgg cagagcgcca actggcccat ttccgccgcc
     5161 actacgcagc gttgatacgc agatgagagc agcaccagcc cgctccccca cccggccacc
     5221 caacgatagt accctagggg tggccgggtg ggggagcggg ctggtgctgc tgcgcccgtg
     5281 agggcgccag ggcaagcacc gggtggggga gtgggccggt gccgctttct caaacgctaa
     5341 tcttttggcc agtcctttat cgaacagttt tcgataacat ccacaccgaa gcatggatcc
     5401 ggccgccggc aaggcgctgc cgcagcgcca tgcaagggga aaggatgtca tgagcaacag
     5461 acccatccat cgccggcaag tgctggccgg cgccggtgcc ggccttgtgg gcgctgccgt
     5521 tgccaccacc gccaccaccg ccaccagcgc ctctgccgcc ggcggggacg atcactggga
     5581 catggaagcc gatatcgtct gcgtcggctc gggcgcgg
//
LOCUS       DASRYE010000086         8539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_5365,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000086 DASRYE010000000
VERSION     DASRYE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5365"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFF98_04805"
     CDS             complement(<1..888)
                     /locus_tag="VFF98_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZU62982.1"
                     /translation="MNADALNASAETAPDRLDADNRLTGDFVRRVREALEDGEAGAVY
                     DLAEGLHPADIADLFEAIPADLRGALADALTDLMGGEVFAELNEHVREALVDELAAAD
                     IADIAEEMETDDAVALIEDLAPEEQRAVLAEMEPEERAAIAAALAFPEESAGRLMSRD
                     VVAVGQHMQVGELIDFLRTRDDLPTEFWEVFIVDALHHPVGTCMLSWILRAPHNVVLG
                     DVMQRDQTLIPVTMDQEEVALRFQKYALISAAVVDESGRLVGQITVDDVLHIIQEEAG
                     EDALALSGAGDGDINEPLLL"
     gene            1128..1595
                     /locus_tag="VFF98_04810"
     CDS             1128..1595
                     /locus_tag="VFF98_04810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU62983.1"
                     /translation="MADEFLTFTIDTGNGTSDVVIKLRPDLAPGHVARIKELAGEGFY
                     DGVKFHRVIPGFMAQGGCPNGTGTGGSDKPDLQAEFNDEPHVRGVCSMARTSYPHSAN
                     SQFFICFDDATFLDRQYTVWGIVESGMETIDALPKGEPPRAPGKIVKATVSAA"
     gene            1751..3637
                     /locus_tag="VFF98_04815"
     CDS             1751..3637
                     /locus_tag="VFF98_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU62984.1"
                     /translation="MFARLKISTLVIVSSLGLAAVVATSAVSTSLGLKSSGERLTYVI
                     TNSLPSINALSRISVMAEVARVRAARVALADGSVASEKDFDDLTKAIGVVEKGLKDYI
                     PLISDAHEQGEYDTVARKWQVEADRLHEIERLARDGHTAEARTLYRGPLVQEAKALRD
                     GMDAEIQYNVDLANQQSEAATASFSQTFYLAVGLGGLALAMAVGMLVLFRQRVSAPLG
                     RLTDTMQEMARGQLDLVVPGAEKGDELGDIARALDGIKRSITERSRAEAEVQLSVQKQ
                     VTGALEEALGALKAGELTHRIRDAFPSEYEVLRSDFNATLASLAEQISEVSRSSAAVR
                     TGAAEIAAAAQDLARRTEAQAATLGETAGTVKELTGAVSEATDAAQGASTAAQDTEKE
                     ASTSGELMREAVAAMNSISATSDKMRSIVEIIDGISFQTNLLALNAGVEAARAGDAGR
                     GFAVVASEVRNLAERSAEAAKEIGALIVNSGQEVRHGVQMVSQTQASLERIVGKASDL
                     ASVIAGISHTASRQSSAITQVNSVIAELDRATQQNAALVEESTAASHSLATESERLSQ
                     VVSRFAFTEGSHARQPAAMPASGGAVPMPARARPARNSAPAPVPMSQGNAALAATSED
                     WSEF"
     gene            complement(3726..4613)
                     /locus_tag="VFF98_04820"
     CDS             complement(3726..4613)
                     /locus_tag="VFF98_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU62985.1"
                     /translation="MPPVRRLPPLRALEAFVRIVRLGSAKAAANELGLSPSALSRRVG
                     ALEDFVGKRLFTRQHQAMKLTDEGQIFYAIVSPKLEELAVAVESQMEQHNVMRLYLGV
                     PPLFAGQRLFPRLPELRKLHPRLHIDIDTGPQLEDRLGDSLDAAIVLSKVPDSAFHAV
                     RLDHNKVYAFAARRIADELGPAPDISRLSRQTFLIQTDLPDSFDAWKVALGLEKLEPA
                     AIDHIDSAQLIITGANQGLGIAIMHSDNFQRAGEETLVKLYDADVESPYSYWFVCRPR
                     ALESRPVRIFHDWLVNAHL"
     gene            complement(4613..5515)
                     /locus_tag="VFF98_04825"
     CDS             complement(4613..5515)
                     /locus_tag="VFF98_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZU62986.1"
                     /translation="MGESGNGEPDSKRSLWRAVWRLFDSSEGDQSLRAQIEGVINEHE
                     GESQATSANGDLLPIERQMLRNLLHFSEHTADDVAIPRGAIMALPLAASWAEVVAAFA
                     EHGHSRLPVHGETLDEVVGMMLMKDVFARLATGDGPPADWHALMRQPLFVPTARGALD
                     VLADMRQSRVHLAVVLNEYSGTEGIITLEDLVEEIVGEVEDEHDDAPTELLHALPDGL
                     WEADARAELDDVAERVDARLADVEEAVDTLGGLAFLLAGHVPQVGETLAHDSGWILEI
                     TDGDERHVKRLRLHPPLSATQGEG"
     gene            complement(5536..6051)
                     /gene="ybeY"
                     /locus_tag="VFF98_04830"
     CDS             complement(5536..6051)
                     /gene="ybeY"
                     /locus_tag="VFF98_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZU62987.1"
                     /translation="MLNLEIDIEGPWPAGTDWADLAFAAAEAAARVAPELANERLFAS
                     LLFTSDDEVHTLNREWRAKDKPTNVLSFPMLEREDLLDLAADGPPEMLGDIALAQETC
                     AREAAEKGIALEAHASHLIIHGLLHLAGLDHEISADDARAMEQLEINALALIGIADPY
                     GDHGDPQGVTS"
     gene            complement(6140..7186)
                     /locus_tag="VFF98_04835"
     CDS             complement(6140..7186)
                     /locus_tag="VFF98_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZU62988.1"
                     /translation="MARKHSRAHDETTSDVAPFPPAGISRPHRRARTELEFEEATVLG
                     ALFGQFDANLVLLENRLGVYISARGNRIQIEGAPDAVARAREVLQGLHKRLLSGQDID
                     AGAVEALISMSSEPVLEGIISGDAERPPIMIRTRKKTIVPRSAMQIAYMQALARDDII
                     FALGPAGTGKTYVAVAQAVAQLINGSVQRLILSRPAVEAGERLGFLPGDMKEKVDPYL
                     RPLYDALYDCMPPEQVERRIASGEIEIAPIAFMRGRTLADAFVILDEAQNTTPAQMKM
                     FLTRFGQNSRMVVCGDPKQVDIPGGPAMSGLGDAVRRLEGVEGIAMVRFGSADVVRHP
                     IVGRIVDAYEGEEA"
     gene            complement(7340..>8539)
                     /gene="miaB"
                     /locus_tag="VFF98_04840"
     CDS             complement(7340..>8539)
                     /gene="miaB"
                     /locus_tag="VFF98_04840"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZU62989.1"
                     /translation="EADLVVLNTCHIREKAAEKVYSDIGRLKREDGTSPLIAVAGCVA
                     QAEGEEIMARAPSVRMVVGPQAYHRLPEMLKAAQGGARVTDTDMPADAKFAALPPRRR
                     TGATAFLTVQEGCDKFCTYCVVPYTRGAEISRPFHDLVAEAERLVEAGAREITLLGQN
                     VNAWTGENAKGQPVGLDGLVRALARLPNLARIRYTTSHPNDMTDALIAAHGEVDKLMP
                     YLHLPVQSGSNRILKAMNRSHTVESYLALLEKVRAVRPDIALSGDFIVGFPGETEADF
                     ADTLALVDQVKYAASFSFKYSPRPGTPAATMAEQIAPAVMDERLQRLQAAIIAGQIAF
                     NQASLGRETDVLVEKRGRHAGQWLGKSPWLQSVHFEGEAAVGDLVRVELVQAGPNSLA
                     GRLIGGA"
BASE COUNT         1414 a         2883 c         2869 g         1373 t
ORIGIN      
        1 gagcagcagc ggctcgttga tatcgccatc gcccgcgccc gacagcgcca gcgcgtcctc
       61 tccggcctct tcctggatga tgtgcagcac atcgtccacc gtgatctggc ccaccaggcg
      121 gccgctttca tccaccaccg cggccgagat cagcgcatat ttctggaagc gcagcgccac
      181 ctcttcctgg tccatcgtca ccgggatcag cgtctggtcg cgctgcatca catcgcccag
      241 caccacattg tgcggcgccc gcaaaatcca gctgagcatg caggtgccca ccggatggtg
      301 cagcgcatcg acgatgaaca cttcccagaa ctcggtcggc agatcatcgc gcgtgcgcag
      361 gaaatcgatc agctcgccca cctgcatgtg ctggcccacg gccaccacat cgcggctcat
      421 caagcgaccg gcgctctctt ccgggaaggc gagggcggca gcgatcgcgg cgcgctcctc
      481 cggctccatc tcggccagca ccgcgcgctg ttcctcgggc gccagatcct cgatcagggc
      541 gaccgcgtcg tcggtctcca tctcctcggc gatatcggcg atatcggccg cggccagctc
      601 gtcgaccagc gcctcgcgca catgctcgtt cagctcggcg aagacctcgc cgcccatgag
      661 gtcggtcagc gcatcggcca gcgccccgcg cagatcggcc gggatcgcct cgaacagatc
      721 ggcgatatcg gccgggtgca gcccttcggc cagatcgtag acagcgcccg cctcgccgtc
      781 ctccagcgcc tcgcgcacgc ggcgcacgaa atcgccggtc aggcggttgt cggcatccag
      841 ccggtcgggc gcggtctcgg cgctggcgtt gagggcgtcg gcgttcatcg ctttcaaggc
      901 tctagggggc tggcgggcaa atgcaagcgg gcaggcagcc cggccggcgc caggggcagc
      961 tggcacgaag ggcgcttccc tttcgcgcca gctggcctat agggcgcaaa ccacgccgga
     1021 aaggggcccc tggtgggcct gtttcggcca acccgatttc cccggcggca aggccggggc
     1081 atgccaatct tccccggcgg caaggccggg gcatgaagga gcaagccatg gctgacgaat
     1141 tcctcacctt caccatcgac accggcaatg gcacgagcga tgttgtcatc aagctgcgcc
     1201 ccgatctggc cccgggccat gtggcgcgca tcaaggaact ggccggcgaa ggcttttacg
     1261 atggcgtgaa gttccaccgc gtgatccccg gcttcatggc gcagggcggc tgccccaacg
     1321 gcaccggcac cggcggctcc gacaagcccg acctgcaggc cgaattcaac gacgagcccc
     1381 atgtgcgcgg cgtgtgcagc atggcgcgca ccagctatcc gcattcggcc aacagccagt
     1441 tcttcatctg cttcgatgat gccaccttcc tcgatcgcca gtacacggtg tggggcattg
     1501 tggaatcggg catggaaacg atcgacgcgc tgcccaaggg cgagccgccg cgcgcgcccg
     1561 gcaagatcgt caaggccacc gtttcggcgg cctgaggcct gcccggcgcc ctgcggggcg
     1621 ccacttcggg caccgtggta aacgaaagct ttaccgcggt gcccggctgc ttgcggaaca
     1681 ggccatcagc ctgtccttga actttctggc ctaagccggg gccatcccac ggcttgccag
     1741 aaagacggat atgttcgcac ggctcaagat cagcacgctt gtcattgtca gctccctggg
     1801 gctggccgcg gtggttgcca cctcggcggt gtccaccagc ctgggcctga agagctcggg
     1861 cgagcgactg acctatgtga tcaccaattc gttgcccagc atcaacgcgc tgtcgcgcat
     1921 ttcggtgatg gccgaagtgg cgcgcgtgcg ggccgcgcgg gtggcgctgg ccgatggcag
     1981 cgtggccagc gagaaggatt tcgacgatct gaccaaggcc atcggcgtgg tcgagaaggg
     2041 gctcaaagac tatatcccgc tgatttccga tgcccatgag cagggcgaat atgacaccgt
     2101 ggcaaggaaa tggcaggtgg aggccgatcg cctgcacgag atcgagcgcc tggcgcgcga
     2161 tggccacacg gccgaggccc gcacgcttta tcgcggcccg ctggtgcagg aggccaaggc
     2221 cctgcgcgac gggatggatg ccgagatcca gtataacgtc gatctggcca accagcagag
     2281 cgaggcggcc acggccagct tcagccagac cttctacctg gccgtgggcc ttggcgggct
     2341 ggcgctggcg atggcggtgg gcatgctggt gctgtttcgc cagcgggtgt cggcgccgct
     2401 gggccggctg accgacacga tgcaggaaat ggcccgcggc cagctcgatc tggtcgtgcc
     2461 gggcgccgag aagggcgacg agctgggcga tattgcccgc gcgctcgatg gcatcaagcg
     2521 ttccatcacc gagcgcagcc gggccgaggc ggaagtgcag ctttctgtgc agaagcaagt
     2581 gaccggcgcg ctggaagagg cgctgggcgc gctgaaggcc ggcgagctga cgcaccgcat
     2641 ccgcgatgcc ttccccagcg aatatgaagt gctgcggtct gatttcaacg ccacgctggc
     2701 ctcgctggcc gagcagattt ccgaagtgtc gcgctcgtcg gcggcggtgc gcacgggggc
     2761 ggcggaaatt gccgcggccg cgcaggatct ggcccgccgc accgaagcgc aggcggcaac
     2821 cctgggcgag acggccggca ccgtgaagga actgaccggc gccgtttccg aggcgaccga
     2881 tgccgcccag ggcgccagca ccgccgcgca ggacaccgaa aaggaagcca gcaccagcgg
     2941 cgagctgatg cgcgaggcgg tggccgcgat gaactcgatc tcggcgacat cggacaagat
     3001 gcgctcgatc gtcgagatca tcgacggcat ctccttccag accaacctct tggcgctgaa
     3061 tgccggcgtg gaagcggcgc gcgcgggcga tgccggccgc ggctttgcgg tggtggccag
     3121 cgaagtgcgc aatctggccg agcgcagcgc ggaagcggcc aaggaaatcg gcgcgctgat
     3181 cgtcaattcc gggcaggaag tgcgccatgg cgtgcagatg gtcagccaga cgcaggcctc
     3241 gctggaacgc atcgtgggca aggccagcga tctggccagc gtgatcgccg gcatctcgca
     3301 caccgccagc cgccagtcga gcgcgatcac ccaggtgaac agcgtgatcg ccgagctgga
     3361 ccgcgccacc cagcagaatg ccgcgctggt ggaggaatca accgccgcct cgcattcgct
     3421 ggccaccgaa tcggagcgcc tgtcgcaagt ggtcagccgc tttgccttca ccgaaggcag
     3481 ccatgcccgc cagcctgcgg ccatgccggc cagcgggggg gccgtgccca tgcccgcccg
     3541 cgcccgcccg gcccgcaaca gcgccccggc gcccgtgccg atgagccagg gcaatgccgc
     3601 ccttgccgcc accagcgagg actggtccga attctgatcc ctggaggggg cggggcgcga
     3661 cgggggcaac cccatcgcgc cccgcttgcg tttgggccgc gggccgctgg caggcagcag
     3721 cccgtttaaa ggtgggcgtt caccagccaa tcgtggaaga tgcgcaccgg ccggctttcc
     3781 agcgcccgcg ggcggcacac gaaccagtag ctgtaggggc tttccacatc ggcatcatac
     3841 agtttcacca gcgtttcctc gccggcgcgc tggaaattgt cggaatgcat gatggcaatg
     3901 cccagcccct gattggcgcc ggtgatgatc agctgggccg aatcgatatg gtcgatcgcc
     3961 gccggctcca gcttttccag gcccagcgcc accttccacg catcgaaact gtcgggcagg
     4021 tcggtctgga tgaggaaggt ctggcgcgag aggcgcgaga tgtcgggcgc cgggcccagc
     4081 tcgtcggcga tgcggcgggc ggcaaaggca taaaccttgt tatgatccag ccgcacggca
     4141 tgaaaggcgc tgtcgggcac cttggagagc acgatggcgg catccagcga atcgcccagc
     4201 cggtcttcca gctgggggcc ggtgtcgatg tcgatatgca ggcgcgggtg cagcttgcgc
     4261 agttcgggca ggcgcggaaa caggcgctgg ccggcaaaca gcggcggcac gcccagataa
     4321 agccgcatca cattgtgctg ctccatctgc gattccaccg ccaccgccag ctcttccagc
     4381 ttgggtgaga cgatggcgta gaagatctgg ccttcatcgg tcagcttcat cgcctgatgc
     4441 tggcgggtga acaggcgctt gccgacgaaa tcctccagcg cgcccacccg gcgcgagagg
     4501 gcagaggggc tgaggcccag ctcgttggcg gcggccttgg ccgaacccag gcggacaatg
     4561 cgcacaaagg cttcaagcgc gcgcagcggc ggcagacggc gaaccggagg catcagcctt
     4621 ctccttgcgt ggcggaaagc ggggggtgca ggcgcagccg cttcacatgg cgctcgtcgc
     4681 catcggtgat ctcgaggatc cagccgctgt cgtgagccag cgtttcgccc acctgcggca
     4741 catggccggc caacagaaag gcgaggccgc ccagcgtgtc caccgcctct tccacatcgg
     4801 ccaggcgcgc atccacccgc tcggccacat cgtccagctc ggcgcgggca tcggcttccc
     4861 acaggccatc gggcagggca tgcagcagct cggtgggggc atcgtcatgc tcatcctcca
     4921 cctcgccgac gatctcttcc accagatcct ccagcgtgat gatgccttcc gtgccggaat
     4981 attcgttcag caccacggcc agatgcaccc ggctctggcg catgtcggcc agcacatcca
     5041 gcgcgccgcg ggcggtgggc acgaacagcg gctggcgcat cagcgcgtgc cagtcggcag
     5101 gcgggccatc gccggtggcc agccgcgcga acacatcctt catcagcatc atgccgacca
     5161 cttcatccag cgtctcgcca tgcacgggca ggcgcgaatg gccatgttcg gcaaaggcgg
     5221 ccaccacttc ggcccagctg gccgccagcg gcagggccat gatcgccccg cgcgggatcg
     5281 ccacatcatc ggcggtgtgt tcgctgaaat ggagcaggtt gcgcagcatc tgccgctcga
     5341 tcggcaacag gtcgccattg gcgctggtgg cctggctttc gccctcatgc tcgttgatga
     5401 cgccttctat ctgcgcgcgc agcgactggt cgccctcgct gctgtcgaac agccgccaca
     5461 ccgcccgcca cagcgagcgt ttactgtccg gctctccatt gccagactca cccatccggg
     5521 ccgcatcggc caccactagc ttgtcactcc ttggggatcc ccatggtccc catatgggtc
     5581 ggctatgcct atcagcgcaa gcgcgtttat ttcaagttgt tccatcgccc gcgcatcatc
     5641 cgccgaaatt tcatggtcca gcccggccag atgcagcagg ccatggatga tgaggtggct
     5701 ggcatgggct tccagcgcaa tgcccttctc ggccgcctcg cgcgcgcagg tttcctgggc
     5761 cagggcaata tcgcccagca tttcgggcgg gccatcggcg gccagatcga gcaggtcctc
     5821 gcgctccagc atggggaagg agagcacgtt ggtgggcttg tccttggcgc gccactcgcg
     5881 gttcagcgtg tgcacttcgt catcgctggt gaacagcagg ctggcgaaca ggcgctcgtt
     5941 ggccagttcg ggcgccacgc gggcggcggc ctcggcggcg gcaaaggcca ggtcggccca
     6001 gtcggtgccg gcaggccagg ggccctcgat gtcgatctca aggttgagca tggcgcaagc
     6061 ccttagaggg gaattgcgcg attggcgagc gccaagaggg ccgcgaggga ggataagccc
     6121 ccctcgcgga cggggccggt caggcctctt ccccctcata ggcatcaaca atccgcccga
     6181 cgatcggatg gcgcaccaca tcggcgctgc caaagcgcac catggcaatg ccttccaccc
     6241 cttccagccg gcgcacggca tcgccaaggc cgctcatggc gggcccgccg gggatgtcca
     6301 cctgctttgg gtctccgcac accaccatgc ggctgttctg gccgaagcgg gtgaggaaca
     6361 tcttcatctg ggcgggcgtg gtgttctgcg cctcgtcgag gatgacgaag gcatcggcca
     6421 gcgtgcgccc gcgcatgaag gcgatcggcg cgatctcgat ctcgcccgag gcgatgcgcc
     6481 gttccacctg ctcgggcggc atgcaatcat acagcgcgtc gtagagcggg cgcaggtaag
     6541 ggtccacctt ctccttcatg tcgccgggca ggaagcccag ccgctcgccc gcttccaccg
     6601 ccgggcggct gaggatgagg cgctgcacgc tgccgttgat caactgcgcc acggcctgcg
     6661 ccacggcgac ataggttttg ccggtgccgg ccgggcccag ggcgaagatg atatcgtcgc
     6721 gcgccagtgc ctgcatatag gcgatctgca tggcgctgcg cggcacgatg gttttcttgc
     6781 gcgtgcggat catgatcggc gggcgctcgg catcgccgct gatgatccct tcgagcaccg
     6841 gctcgctcga catcgagatc agcgcctcga ccgcgccggc atcgatatcc tggccggaaa
     6901 gcaggcgctt gtgcaggccc tgcagcactt cgcgggcgcg ggccacggca tcgggcgccc
     6961 cttcgatctg gatgcggttg ccgcgggccg agatatagac cccgagccgg ttttccagca
     7021 gcaccagatt ggcatcgaac tggccgaaca gggcgcccag cacggtcgcc tcctcgaatt
     7081 ccagctctgt gcgggcgcgg cggtgcgggc gggagatgcc tgcgggcggg aagggcgcca
     7141 catccgatgt ggtctcatcg tgggcgcggg aatgcttgcg tgccatgcgc tccttttctg
     7201 cggccggaat cggaagaagg cccaccggct gcaggcaaag tctatgtcag cgcccctgcc
     7261 aggcaaagcg ggaatggcgc gggcggctgt ggataaatca gccggtgtcg atcaggcgcc
     7321 gccaatcagc cggcgttgat caggcgccgc caatcaggcg gccggccagc gaattggggc
     7381 cggcctgcac cagttccacc cgcaccagat cgcccacggc cgcctcgccc tcgaaatgca
     7441 ccgattgcag ccagggcgat ttgcccagcc attgcccggc atggcgcccg cgcttttcca
     7501 ccagcacgtc ggtctcgcgg ccaaggctgg cctggttgaa ggcgatctgc ccggcgatga
     7561 tcgccgcctg caggcgctgc agccgctcgt ccatcaccgc gggcgcgatc tgttcggcca
     7621 tggtggcagc gggcgtgccg gggcggggcg aatatttgaa gctgaaggaa gcggcgtatt
     7681 tcacctgatc caccagcgcc agcgtatcgg cgaaatcggc ttccgtctcg cccgggaagc
     7741 ccacgatgaa atcgcccgac agcgcgatat cgggccgcac cgcgcgcacc ttttccagca
     7801 gcgccagata gctttccacc gtgtggctgc ggttcatcgc cttgaggatg cggttgctgc
     7861 ccgattgcac cggcagatgc agatagggca tcagcttgtc cacctcgcca tgggcggcga
     7921 tgagcgcgtc ggtcatgtcg ttggggtggc tggtggtgta gcggatgcgg gccagattgg
     7981 gcaggcgggc aagggcgcgc accagcccgt caagccccac aggctggccc ttggcattct
     8041 cgccggtcca ggcgttcaca ttctggccga gcagggtgat ctcgcgcgcg ccggcttcca
     8101 ccagcctctc ggcttctgcc acaagatcat ggaaggggcg cgatatttcc gcgccgcgcg
     8161 tatagggcac cacgcaatag gtgcagaatt tgtcgcagcc ttcctgcacg gtgaggaaag
     8221 cggtggcgcc ggtgcggcgg cgggggggca gggcggcaaa cttggcgtcg gccggcatgt
     8281 cggtgtcggt cacgcgggcg ccgccctggg cggccttcag catctcgggc aggcgatgat
     8341 aggcctgcgg gcccaccacc atgcgcaccg aaggcgcgcg cgccatgatt tcctcgccct
     8401 cggcctgggc cacgcagccg gccaccgcga tcagcgggct ggtgccatcc tcgcgcttca
     8461 ggcggccgat gtcggaatag accttctcgg cggccttctc gcggatatgg caggtgttga
     8521 gcacgacgag atcggcctc
//
LOCUS       DASRYE010000087        32769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_146623,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000087 DASRYE010000000
VERSION     DASRYE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 32769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32769
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146623"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="dnaN"
                     /locus_tag="VFF98_04845"
     CDS             complement(<1..791)
                     /gene="dnaN"
                     /locus_tag="VFF98_04845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZU62990.1"
                     /translation="MKATLERATLLRCLGHVQSVVERRNTIPILSNVLIEAGGDGSLK
                     VMATDLDLQVVEFMSAVSVEAPGSITVSAHLLFDIARKLPDGAQVQLQTAENRLVVKA
                     GRSRFTLPTLPRDDFPVIVEGDLPTSFELPAATLAQLIDRTRFAISTEETRYYLNGIF
                     LHVTEDELKGAATDGHRLARFTLARPDGAEGMPDVIVPRKAVAELRKLLEEALDTAVQ
                     IDLSASKIRFTLAGQNGVVLTSKLIDGQFPDYTRVIPTGNDKLLRL"
     gene            complement(1110..1850)
                     /gene="fabG"
                     /locus_tag="VFF98_04850"
     CDS             complement(1110..1850)
                     /gene="fabG"
                     /locus_tag="VFF98_04850"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZU62991.1"
                     /translation="MFSLEGMTALVTGASGGIGSAVARALAGQGARLALSGSNADKLE
                     AFAAELGGDHVCVVANLADKDSVEALVPAALEKLGKLDILVNNAGITRDNLAMRMKDE
                     EWEAVIRLNLEGAFRLMRAATKPMMKARFGRIVNITSVVGATGNPGQVNYAAAKAGLV
                     GASKSLAQELASRNVTVNCVAPGFIRTAMTDVLPDAQKEALNARIPMGRMGEGEDIGA
                     AVAYLASKEAGYVTGQTLHVNGGMAMFA"
     gene            complement(1850..2803)
                     /gene="fabD"
                     /locus_tag="VFF98_04855"
     CDS             complement(1850..2803)
                     /gene="fabD"
                     /locus_tag="VFF98_04855"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZU62992.1"
                     /translation="MRAFVFPGQGSQKVGMGVELAEASAIAREVFQEVDEALGQNLLR
                     LMAEGPEDQLTLTENAQPAIMANAIATLRVAEAEGGITLADKADFVAGHSLGEYTALC
                     AAGAFSLADTARLLKLRGQAMQAAVPVGVGAMAALLGADIDKATALAQAAAVAPDGTS
                     EVCAVANDNDPGQVVLSGHRGAIERAIALVKEHGIKRGVALPVSAPFHCPLMQPAADA
                     MAEALAKTAPGALRVALYANVTADVVTDPAVVQRLLVEQVCGRVRWRESVIAMQAAGV
                     AEFVELGGKVLGPMIGRSVKDVKVTSVVTMADIEALLKEIA"
     gene            complement(2838..3671)
                     /locus_tag="VFF98_04860"
     CDS             complement(2838..3671)
                     /locus_tag="VFF98_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZU62993.1"
                     /translation="MTIRIAICAPATPITPEDVARAQAVADSVGGVELNVHPQCFLAE
                     GHFAGSDAQRLEAFLDCAHDPGTDAVWFARGGYGSNRIAGAAVARLGAAAAGKVFLGY
                     SDMGYLLGALYRHRIGRPCHGPMLADGRRQEGAAALARALAFLKGEGGGLEPSLAGST
                     HPAVAFNLTTLAMLVGTPLMPGLAGHVVMVEEVSEYLYAVDRLFFHATAHLAGIAGLR
                     LGRVSDVPENDRPFGASAEAIAQDWCARHAIPFLGAADIGHDADNRIVPFGLAPARVR
                     Q"
     gene            3727..5142
                     /locus_tag="VFF98_04865"
     CDS             3727..5142
                     /locus_tag="VFF98_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZU62994.1"
                     /translation="MADSALTSHPWFFCGIGGSGMLPLALILKGLGAEVAGSDRGRDQ
                     GLTPEKFAWLEGLGLRLFPQDGSGLAAPDQVLVASAAVEDSVPEVVRAQQLGCPRMSR
                     AELLARLFNAAPARIAIGGTSGKSTVTGMTGWILTATGRDPTIMNGAVMKNFATPEAP
                     FASARVGQGSLFVSEVDESDGSIALYQPTVAVLSNVSLDHKSLEELRALFGAFLARAD
                     IGVVNLDNAEAAALAPQARRLVSFGIDSAAADLGVVPGSIVEGATQIAATLVDRRCGA
                     THALALRVPGRHNLANALAALAAASAAGVPLAEAVAALGAYAGLARRFDIVGTSPSGI
                     TVIDDFGHNPDKVAATLATLKAHEGRVIAFFQPHGYGPLRQMGAELAATFARLLGPGD
                     EAILCDPVYFGGTVDRSLGAERIVALIGEAGGHARHIPARADVATHIAAVARPGDRIV
                     VMGARDDSLSRFARELLAALP"
     gene            complement(5223..6221)
                     /locus_tag="VFF98_04870"
     CDS             complement(5223..6221)
                     /locus_tag="VFF98_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62995.1"
                     /translation="MPLCDAGRLNGLVALADAVALIQAGAPLAIAGRPEALAQLPQGR
                     WIAGTTPYFMTAEGGQVIDATRVFVTDLSGLGGVSVGTYDADSLHLISEEAPENGFAL
                     AIIPFQSAVHERFAREASTYPQAFLKPTVGWIAGFDLAEAGGHALVYDGLTGTAHANR
                     VAVMHVELPEEYLPQVEIVNLFEADGVDVIRFEEAGFAPAQVVVNGQSQLFADYVRGR
                     GLADGRLPLVGDYAGARVNASIRAVEGDSVSLYAPVFPGINYAFARPVADYAAAFRDQ
                     LEATPHEGALWSCNCILNFLYGELEGKAIGGVAGPVTFGEIAYQLVNQTLVQLRAI"
     gene            complement(6208..6423)
                     /locus_tag="VFF98_04875"
     CDS             complement(6208..6423)
                     /locus_tag="VFF98_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62996.1"
                     /translation="MALPSKSAPCWLKLADGGVKRLKTTNLGVQMLAQRLELSPMSKA
                     DKAGEFFNFFTKWEKGLGNEIAQLASL"
     gene            6632..7024
                     /locus_tag="VFF98_04880"
     CDS             6632..7024
                     /locus_tag="VFF98_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62997.1"
                     /translation="MLLNSMADAVVYVPRAERVSFDMLVRYRLAAMRATVWLKNLTCG
                     GARIEGMALATGDVLALYLPSLKPKNARVAWTVGTSAGLEFERPLHPDIFESLVLRHA
                     RPRERTETDRLLALDGGPALPAAALVPA"
     gene            7262..7651
                     /locus_tag="VFF98_04885"
     CDS             7262..7651
                     /locus_tag="VFF98_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU62998.1"
                     /translation="MPRHFTFAATLALLSLPLAACSAGNVHTNRGFAALSKTHYADAE
                     SELKLALQDNPNDPFALLNMGAVLQNTGRGQQAVPYYLRVQAEAKHVYPQRTSGDTSA
                     RHSLAEIATQNLTKLAANDSSIRVTAN"
     gene            complement(7727..9151)
                     /locus_tag="VFF98_04890"
     CDS             complement(7727..9151)
                     /locus_tag="VFF98_04890"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZU62999.1"
                     /translation="MSLSRPIIAELVTLYEAAVATLRADIAAFADHGTLPPPDRHLER
                     AWCYPELRISYAGGVIRPDYARAFGRLVHAGAYATTITRPALFADYLAEQLDLLMADY
                     EVDVTVGRSAQPIPFPYVLDAAAGISARPIDLAQHFPTTDLAEIGDELADGAFLPGEA
                     RPLSLFDGPRTDFSLARLAHYTGTNPEHFQRFILFTNYHRYVDEFVDWAGGQLGQNGY
                     EKLSGAGGLLVEKPVDNARALLSDTAWRRHQMPAYHLTRADRGGISLVNIGVGPSNAK
                     TICDHLAVMRPEAWLMIGHCGGLRDTQRIGDYVLAHAYLRDDHVLDPVLPPEIPIPAI
                     AEVQLALAHAAELVSGETGANLKKRMRTGTVVTTDDRNWELRYTASARRLSLSRAVGI
                     DMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEEAIAAHLKIGTTTC
                     ELLRAEGPRLHSRKLRAFNEPAFR"
     gene            complement(9273..9872)
                     /gene="rplI"
                     /locus_tag="VFF98_04895"
     CDS             complement(9273..9872)
                     /gene="rplI"
                     /locus_tag="VFF98_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZU63000.1"
                     /translation="MQIILLERIEKLGTIGDVVTVKDGYARNFLLPNKKALRANEANR
                     KVFEAQRAEIEARNAARREEAQGEAGSVDGKQAVLIRAASNAGQLYGSVSVRDIADAL
                     NTEGAKVTKAMIVLERPIKTIGIFDVRVALHPEVSVMVKVNVARSPDEAELQTQGVNV
                     IDALFDNEVGGFTEAYDPNAEPGEIPAELLDEPAEGAEG"
     gene            complement(9885..10109)
                     /gene="rpsR"
                     /locus_tag="VFF98_04900"
     CDS             complement(9885..10109)
                     /gene="rpsR"
                     /locus_tag="VFF98_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZU63001.1"
                     /translation="MARAFFRRRKSCPFSGKNAPKIDFKDVRLLQGFMSERGKIVPSR
                     ITAVSAKKQRELSQAIKRARHVGLLPYLVK"
     gene            complement(10113..10475)
                     /gene="rpsF"
                     /locus_tag="VFF98_04905"
     CDS             complement(10113..10475)
                     /gene="rpsF"
                     /locus_tag="VFF98_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZU63002.1"
                     /translation="MPLYEHVFLARQDLSQSQVDALAATATEIIEANGGKVTKTETWG
                     LKNLAYKIKRNRKAHFVMLNVEAPAGVVAELERQIGINEDIIRYITVRVDELETGPSV
                     QMRKNDRERSRRREREEA"
     gene            complement(10642..11421)
                     /locus_tag="VFF98_04910"
     CDS             complement(10642..11421)
                     /locus_tag="VFF98_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU63003.1"
                     /translation="MTLEVTSRFLPGFDGALLAVHEAGPAEGRPVLLLHGLFSTAEVN
                     WVKYGHAGKLAAAGFRVIMPDLRGHGRSAAPHAPEAWPEDVLVKDVLALAPQLGLAPG
                     GFDLGGFSLGARTSVRSVLAGLQPARLILGGMGLEGLAGWARRSAFFIDAIDRYGTIK
                     HGDPAYVAQQFMKAQKIDLVAARLLLHSVDDTEPDDLIALKQPVLVVCGDEDLDNGSA
                     PRLARLLVHARYVEVPGTHMSSVTRPELGDALLAFLAEKPA"
     gene            complement(11430..12188)
                     /locus_tag="VFF98_04915"
     CDS             complement(11430..12188)
                     /locus_tag="VFF98_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HZU63004.1"
                     /translation="MRYQLHGTTMQTVAIDLARGDSLYSQSATMAWMTDGVRMDTHTG
                     GGLMAGLKRMFSGSGLFVTEFTAETDSHIAFAPRFPGQILPVELAPGQALICRKETFL
                     CAQGSVALDIFFQQRLGAGFFAGEGFILQKVTGPGVVFLDLSGEVVERNLAPGERLHV
                     HAGHIGMQDPTVQVDIRMLRGFRNILFGGEGLFLATLTGPGRVWLQSMPIINLAEEIG
                     RYLPREGGDTSGRIGAGAAGAVMGGLLGGLLGNE"
     gene            complement(12355..13380)
                     /locus_tag="VFF98_04920"
     CDS             complement(12355..13380)
                     /locus_tag="VFF98_04920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZU63005.1"
                     /translation="MGYRVVVVGATGNVGREMLNILAEREFPIDEIAAVASPRSQGTE
                     IDFGETGKKLKVQNIEHFDFTGWDIALFSAGSGPTKIYAPKAAAAGCVVIDNSSLYRM
                     DPDVPLIVPEVNPEAIDGYKAKNIIANPNCSTAQMVVALKPLHDKAKIKRVVVATYQS
                     VSGAGKEGMDELFEQSRAIFVGDPVEPKKFTKQIAFNVIPHIDVFLDDGFTKEEWKMV
                     AETKKILDPKIKLTATCVRVPVFIGHSEALNIEFENEISAEEAQDILREAPGIMLVDK
                     REDAGYVTPVECVGDYATFVSRVREDSTVENGLALWCVSDNLRKGAALNAVQIAELLG
                     RRHLKKG"
     gene            complement(13461..15620)
                     /locus_tag="VFF98_04925"
     CDS             complement(13461..15620)
                     /locus_tag="VFF98_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZU63006.1"
                     /translation="MADAHPPLDFARVFASPALAGSAPVQPRIAPGGGWVAVLRPREG
                     ERSRHDLWGWERASGQWRMLVDSTRLEEGAALSEAEKMRRERLRLGERRGILSFGWTR
                     DGTALVVPTPGALWLAGLDGALERLDTGTGAIDARLSPAGGYVSYLAEGRLWVAPRGR
                     LASAITPAESADTVRWGEAEFVAQEEMARLSGYWWAPDDSRLAVQRFDESGVAEVLRA
                     SIGGEGTAMVRQRYPAAGTANAAVALWLMAPDGSDAVRVDLGEGADVYLARVDWAPDG
                     RALYVQRQDRAQGRLDMLKVDPATGAASLLFTEEAAPGHWINLSDAYRFLANGRLVWW
                     SERDGHGHLWLLADGAWRQLTDGAWDVTDLAGVDEAGERLFFAATRDDVLAQQIYALD
                     YSTPGAGPERLTQDGFVHAASMDAAGQAMIVTRHGPDQPPQAYLADGQGTRLAWLAEN
                     RVEGNHPYAPHRAAHRLPQFGTLEAEDGTTLHWRMILPEMVAGQRYPVFFEHYGGPHV
                     QLVQRGWTGALAQAIVAMGYIHFQIDNRGSAHRGVAFESAIRMAMGTPEVADQRAGAR
                     YLQGLPFVDPGKIATYGWSYGGYLTLKMLAADQGLYAAGIAGAPVTRWQLYDTHYTER
                     YLGDPRLVPEVYDRANALDGLTALADPLLLIHGLADDNVVFAHSAEAIARLQANAQAF
                     EMMLYPGQAHGVGGPGVSEHLWNTIFRFLARHGVTPP"
     gene            complement(15725..16096)
                     /gene="rplS"
                     /locus_tag="VFF98_04930"
     CDS             complement(15725..16096)
                     /gene="rplS"
                     /locus_tag="VFF98_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZU63007.1"
                     /translation="MNLIQQIEAEEIAKAAKEIPEFRPGDTVRVGVKVIEGERTRVQA
                     YEGVVIARSNRGINSNFTVRKMSFGEGVERVFPLYSPNIDSIAVVRKGVVRRAKLYYL
                     RGRTGKRARIAERRDTRGQEG"
     gene            complement(16093..16839)
                     /gene="trmD"
                     /locus_tag="VFF98_04935"
     CDS             complement(16093..16839)
                     /gene="trmD"
                     /locus_tag="VFF98_04935"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZU63008.1"
                     /translation="MAFAATVLTLYPEMFPGPLGVSLAGRALAEGKWTLDPVHIRSFA
                     EDKHRTVDDTPAGGGAGMVLKVDILAKAIDHARASHPDCPVVAMTPRGKPLTQARVRE
                     LAAGPGVTILCGRFEGFDERIFAGRQVEEISVGDIVLSGGEPAALMLLDACIRLLDGV
                     MGAASSGDEESFESGLLEYPHYTRPTEWEGHMIPEVLRSGDHGKIAAWRKQQALADTR
                     LRRPDLWERHSGVRGQSASGARQETKDLGQ"
     gene            complement(16843..17385)
                     /gene="rimM"
                     /locus_tag="VFF98_04940"
     CDS             complement(16843..17385)
                     /gene="rimM"
                     /locus_tag="VFF98_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070962.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZU63009.1"
                     /translation="MAGGRPERMIPMAAVAAAHGVAGEVRLKLFGESAAGLKRYKAFN
                     GGALSPTALRDDGKGGAIARFAEIADRTAAEKLRGTLLSVPRSALPPLAEGEYYHADL
                     IGLAAVSDAGAALGVVVAVENFGAGDIVEIEAPAAAGAKPARFMVPMRPDAVPAWDEE
                     RLIVAAAYADNDADAGAQEG"
     gene            complement(17385..17759)
                     /gene="rpsP"
                     /locus_tag="VFF98_04945"
     CDS             complement(17385..17759)
                     /gene="rpsP"
                     /locus_tag="VFF98_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZU63010.1"
                     /translation="MSVSLRLSRGGSKKRPYYKIVIANSTAPRDGAFLEQVGTYNPLL
                     AKDDENRVRLIEDRVRYWLSVGAQPTDRVARLLDKAGVKERKATNNPNKAEPGKKAKE
                     RAEEKAEKAAAAAEAAAEAAAE"
     gene            complement(17794..19269)
                     /gene="ffh"
                     /locus_tag="VFF98_04950"
     CDS             complement(17794..19269)
                     /gene="ffh"
                     /locus_tag="VFF98_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZU63011.1"
                     /translation="MFDNLSDRLGGVFDKLRGRGALKEQDVRDAMREVRIALLEADVA
                     LPVVRRFIDEVTEKAIGQNVLKSVTPGQQVVKIVNDALVAMLGGEDVPGLDLAATPPV
                     VIMMVGLQGSGKTTSTAKLGKFIREKLGKKVLMASLDVNRPAAQEQLAVLGTQVDVAT
                     LPIIAGQTPTAIAERAVNSAKLQAVDVLLLDTAGRLHVDAALMDEMKAVAAIASPKET
                     LLVVDSLAGQDAVNVAQSFAQNVALTGVILTRLDGDARGGAALSMRAVTGKPIKFAGM
                     GEKLDQLEVFSPGRIAGRILGMGDVVSLVEKAAAAVEADEAERLAQRMAKGQFDMTDL
                     QTQLRQMQKMGGLGMLAGMLPGMKKAKAAMDASGMSDRTLLRMDAIVSSMTPKERAKP
                     DLLNAKRKIRIAKGAGVQVQDVNKLIKMHQEMERAMKQIKKMGGLKGLAAMFGGGGGL
                     GGLGGGGAAGGVPGGGLPGGLGGMPGLGGGGAPDFMKFLKK"
     gene            complement(19463..19538)
                     /locus_tag="VFF98_04955"
     tRNA            complement(19463..19538)
                     /locus_tag="VFF98_04955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19503..19505),aa:Ala,seq:ggc)
     gene            complement(19566..19641)
                     /locus_tag="VFF98_04960"
     tRNA            complement(19566..19641)
                     /locus_tag="VFF98_04960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19606..19608),aa:Ala,seq:ggc)
     gene            complement(19853..20170)
                     /locus_tag="VFF98_04965"
     CDS             complement(19853..20170)
                     /locus_tag="VFF98_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HZU63012.1"
                     /translation="MVKPEWGAKHGCPKCGTRFYDLGKDDPVTCIECGYAWTPEPVLK
                     SKQPIPYEEIQKKEGPEEADVDLADEDLDIDDDGDSPDNDVDLGGDDDLGVGGHDEEP
                     DEV"
     gene            20403..21704
                     /gene="aroA"
                     /locus_tag="VFF98_04970"
     CDS             20403..21704
                     /gene="aroA"
                     /locus_tag="VFF98_04970"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZU63013.1"
                     /translation="MPAGALAGTIRVPGDKSISHRSIMLGALALGETRVTGLLEGEDV
                     MSTAAAMRAMGATIEKQANGEWVIHGVGVGGLLQPETALDMGNSGTSTRLLMGLVASH
                     AITATFIGDASLSKRPMGRVITPLSEMGAQFTASEGGRLPLTMRGAQPAVPITYRLPV
                     ASAQVKSAVLLAGLNTPGITTVIEPVPTRDHSERMLQGFGADLWVEEDAGERVIRLRG
                     EAELTGQTIEVPGDPSSAAFFIVAALIVPGSDVLIENVGLNPTRAGIITVLRQMGGQI
                     EEVSPRIVGGEPVADLRVRHSALKGIVVDPAIVPSMVDEFPVFFVAAALAEGRTITTG
                     LEELRVKESDRISAMHAALALAGAATEETADGLIIDGTGGAPLRGGANTPVVTHLDHR
                     IAMSMAIAGLVSTAGVEVDDTGPIATSFPNFLALLATLTGA"
     gene            21708..22004
                     /locus_tag="VFF98_04975"
     CDS             21708..22004
                     /locus_tag="VFF98_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZU63014.1"
                     /translation="MALVPAIPEALATAAGFAGMALCVGAYAYTTAYTGRPRGPNPYL
                     QHGGNLAGALLLVVSLLVDTNPASLVLESVWALIAATGLLRALMAGKAAQRSGA"
     gene            22001..22624
                     /locus_tag="VFF98_04980"
     CDS             22001..22624
                     /locus_tag="VFF98_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZU63015.1"
                     /translation="MIIAVDGPTASGKGTIAKALAARFGLPHLDTGLLYRAVGRQVAL
                     QGGDPDSPVDALAACGFADALLADPELRSEASGGLASRVSVHHDVRAALYERQRAFAT
                     QAGGAVLDGRDIGTVIAPEAEVKLFVIASVAARAERRHAEMLAQGRDVALAAIAADLE
                     ARDRRDATRAEAPLRAAVDAITLDTSTLGREEAIAAAIAAVRQRCPA"
     gene            22874..24571
                     /gene="rpsA"
                     /locus_tag="VFF98_04985"
     CDS             22874..24571
                     /gene="rpsA"
                     /locus_tag="VFF98_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZU63016.1"
                     /translation="MSIPSRDDFAALLDESLGGAASGGFEGRVVKGTVTAIENDKAVI
                     DVGLKSEGRVALREFAAPGQPHGLKVGDEVEVYVDRVENADGEAMLSRDRARREAAWD
                     KLESEFGEGKRVEGVIFGRVKGGFTVDLDGAVAFLPGSQVDIRPVRDVTPLMDVPQPF
                     QILKMDRRRGNIVVSRRAVLEETRAEQRSGLIQNLSEGQILDGQVKNITDYGAFVDLG
                     GIDGLLHVTDMSYKRVNHPSEVIAIGDTVRVQIVRINHETQRISLGMKQLESDPWDGV
                     AAKYPVGAKLSGVVTNITEYGAFVELEPGIEGLVHVSEMSWTKKNVHPGKIVSTSQEV
                     EVMVLEVDSDKRRISLGLKQAQQNPWEAFAERHPVGSTVEGEVKNATEFGLFIGLDGD
                     VDGMVHMSDIAWGISGEDALALHRKGEQVSAIVLDVDVEKERISLGMKQLEKGAPTGG
                     NSAGSNLRRNEVVTVTVLEVRDGGIEVQAGEDGATGFIKRSDLGRDRDEQRPDRFQVG
                     QKIDAMVTGFDRSKKPTFSIKARQLHEEKEAVEQYGSSDAGASLGDILGAALKASKN"
     gene            24725..25501
                     /gene="gloB"
                     /locus_tag="VFF98_04990"
     CDS             24725..25501
                     /gene="gloB"
                     /locus_tag="VFF98_04990"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZU63017.1"
                     /translation="MPLLIHQFPCLADNYGFLVHDPASGETVAIDTPDAEEYLRQAEA
                     QGWAITQIWNTHWHRDHAGGNLAIKAATGARITAPAGDAAKIEGVDRVVDQGDSVAIG
                     ACTGHVLNVGGHTLGHVAYHLPGAAVDGGDIAFVGDALFALGCGRMFEGEAAQFWASL
                     QRLKALPGATWLYCAHEYTASNARFARHADPDNAALATYAAEVDGKRADGLPTVPFPL
                     ARELVTNPFLRADDPALQAHWGGGDAVATFAALREAKNGF"
     gene            complement(25702..26244)
                     /locus_tag="VFF98_04995"
     CDS             complement(25702..26244)
                     /locus_tag="VFF98_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63018.1"
                     /translation="MADAASLFLLAAGANAGEAAEPSALGLAPGAWVALAMVVLLLVA
                     LKAKVPGVITKGLDGKIAEIRKQLDEAKALRAEAEELRAQYARRIANAEKDAAEMLAH
                     AKSEAEAIIAKAEASTTEVIARREKIAADKIEAAERAALAELQQKTAQAAAGAARSLI
                     AAGYGVAQDKAQVDEAIARL"
     gene            complement(26237..26728)
                     /locus_tag="VFF98_05000"
     CDS             complement(26237..26728)
                     /locus_tag="VFF98_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZU63019.1"
                     /translation="MPQIAQLSETYASQIFWLLLFFGFTFFVVGRGMVPKVMATVASR
                     EARIAEDLAAAEAARIAAEQDEVHSQRDGARQRAEAQAVIAKAKHDAALATEARLAEA
                     AGKVDGRIAEADARIAHARAAALGEIETVAAEAASDIASKIAGLSVDAGQARAAVKEI
                     LHG"
     gene            complement(26927..27157)
                     /locus_tag="VFF98_05005"
     CDS             complement(26927..27157)
                     /locus_tag="VFF98_05005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZU63020.1"
                     /translation="MEPASAKLIGAGLAAIGAGIAAIGVGNVFGSFLESALRNPGAAD
                     GQQGRLFIGFAAAELLGLLSFVVAMILIFVAK"
     gene            complement(27266..28021)
                     /locus_tag="VFF98_05010"
     CDS             complement(27266..28021)
                     /locus_tag="VFF98_05010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZU63021.1"
                     /translation="MQQFTIEPILGSNWQLAGHNIAFTNSALWMAIVTIALWAFVAGG
                     MKRALVPGRWQMAVESFTGFIDSLLAANIGPAGKKYVPYIFSLFMFILFSNLLGLLPL
                     SLVGIHPFTVTSHFTVTGVLAIISFAIVLIVGFGKHGLHFFSLFVPHGTPLPMIPLIF
                     CIELVSFLVRPFSLGLRLFVAMMAGHVLLEVLSSFVITSANSGVLWGGVVGLPSFALM
                     IAIQALEILVGGIQAYVFALLTSLYLNDAENLH"
     gene            complement(28146..28454)
                     /locus_tag="VFF98_05015"
     CDS             complement(28146..28454)
                     /locus_tag="VFF98_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZU63022.1"
                     /translation="MDESRPSGGPDSEEARLAALDERLRAVREREAARNQPKGHATDA
                     NYRLGSKVLAELLGGIGGGALVGWTIDRLAGTAPWGLLVMMFLGIIVAFRNIIRISGG
                     "
     gene            28963..29670
                     /gene="radC"
                     /locus_tag="VFF98_05020"
     CDS             28963..29670
                     /gene="radC"
                     /locus_tag="VFF98_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZU63023.1"
                     /translation="MAHPLPDRIAAAAHDPTGHRARLRKRLLEGGQDALADHELIEYL
                     LATAIPRRDTKPLARTLLARFGSLAGIFNADPAALAGHPGMGESSAAAVHVVAVAARR
                     LGRARVSEKPVLGSWQALISYLTIDMAHLTVERVRVLYLDTKNRLIRDEHVTDGSVDS
                     AEIHPREVIHTAMNLGAAGLILVHNHPSGNPEPSRADIQITLRIAEAGRNLGITVHDH
                     VIIGREGHVSLRAKGLI"
     gene            29887..30939
                     /locus_tag="VFF98_05025"
     CDS             29887..30939
                     /locus_tag="VFF98_05025"
                     /inference="COORDINATES: protein motif:HMM:NF020704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HZU63024.1"
                     /translation="MVIRSGTSISAQRNRTAAAPQPRRNVLIEGEHPMVVLEDLAARP
                     RPDAHIIVLANEKGGVGKSTVAFHTCMALCDQGFKVAAVDLDFRQQSLARVLAFREAT
                     ARRLGIELPSPQYTAMHHTSGALLCQELARVGWHADYIVIDVAGHDSPLARRAIALAN
                     TLVTPVNSSFVDLDLLGRFNPSTQELLGPGFFANMVNELREERLRRDMGTIDWVVMQN
                     RVRRGISNNQARVDGALQCLAPRAGFRLGQGLTERVAYRELFLLGLTHLDLRHIPDLS
                     HMRAQARDEIFGLLEDMGLPRPDAPGAPGARALDEAAHLAAEALAAAQAQSSLAHSSQ
                     GSTTRALAAHRSEASA"
     gene            31106..32419
                     /gene="purB"
                     /locus_tag="VFF98_05030"
     CDS             31106..32419
                     /gene="purB"
                     /locus_tag="VFF98_05030"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZU63025.1"
                     /translation="MVPRYARPAMTAIWEPEARYGIWFEIEAHATDKLGELGVVPASA
                     GKALWDWWATGPKIDVAAIDAIEAVTKHDVIAFLTWVAEQVGDEARFMHQGMTSSDVL
                     DTTLNVQLVKAADLLLEDLDALLAAIKRRALEHKYTPTIGRSHGIHAEPTTFGKKLAE
                     AFAEFTRCRARLVAARAEVATCAISGAVGTFANIDPGVEEYVAEKMGLEVEPVSTQVI
                     PRDRHAMFFATLGVIASSIERLAVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPV
                     LTENLTGLARMVRSAVVPAMENVALWHERDISHSSVERFIGPDATITLDFALARLTGV
                     VDKLLIYPARMQKNLDRMGGLVHSQRVLLALTQAGISREDSYRLVQRNAMKVWESDGQ
                     LSLLELLKADPEVAAKVPPAELEEKFNLDYHFKAVDRIFERVFGA"
     gene            32548..>32769
                     /locus_tag="VFF98_05035"
     CDS             32548..>32769
                     /locus_tag="VFF98_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HZU63026.1"
                     /translation="MQFVRTGITVVVLAALVVFSLNNWTPVEVRIWDGLVLDTRLPAL
                     MLLSWLVGWLPMWLVSKAARWRLKRRIALL"
BASE COUNT         5518 a        11432 c        10431 g         5388 t
ORIGIN      
        1 agccgcagca gcttgtcatt gccggtgggg atcacgcggg tgtaatcggg gaactgcccg
       61 tcgatcagct tgctggtcag caccacgccg ttctggccgg ccagggtgaa gcggatcttg
      121 ctggccgaca ggtcgatctg cacggcggtg tccagcgcct cttccagcag cttgcgcagc
      181 tcggccacgg ctttcctggg cacgatcaca tcgggcatgc cttcggcgcc atccgggcgg
      241 gccagggtga agcgggccag gcgatggcca tcggtggcag cccccttcag ctcatcttcc
      301 gtcacatgca ggaagatgcc gttgaggtag tagcgcgttt cctcggtgct gatggcaaag
      361 cgggtgcgat cgatcagctg ggccagcgtt gccgccggca gttcaaagct ggtcggcaga
      421 tcgccttcca cgatcaccgg gaaatcatcg cgcggcaggg tgggcagggt gaagcggctg
      481 cggccggcct tcaccaccag gcggttctcg gccgtctgca gctgcacctg cgcgccatcg
      541 ggcagcttgc gggcgatatc gaacagcaaa tgcgccgata cggtgatcga accgggcgct
      601 tccacgctca ccgcgctcat gaattccacc acctgcaggt cgaggtcggt ggccatcacc
      661 ttgagcgaac catcgccgcc cgcctcgatc agcacgttgg agaggatggg gatcgtgttg
      721 cgtcgttcca ccaccgattg cacatggccg aggcagcgca gcaaagtggc gcgttccagt
      781 gtcgccttca tgccagattg cccttcatca atgtcccaat cccagcctca aggcctgcgg
      841 cccttgcggt gccggcgcgg cgcggttgcc catgcgaaaa cggcccctgc gcggaatcgc
      901 cggcccgttt tccacgaaat tcggaccacc ttccttaacg caagccccgc cctcggcaag
      961 ccttatcctg aaggccgcgc gcgtttctgg tgataattct gcgggaaatc ggggatatgc
     1021 aggcaaatgc ccccgcatgc cggggcagcg ggggcatttt ctctcaggcg aggatcgtgc
     1081 ctgctgtcag gcgaacatcg cccaggccgt caggcgaaca tagccatgcc gccgttcaca
     1141 tgcagcgtct gcccggtcac atagccggct tccttgctgg cgagataggc caccgccgcg
     1201 ccaatatcct cgccctcgcc catgcggccc atggggatgc gcgcgttcag cgcctccttc
     1261 tgggcatcag gcagcacgtc ggtcatcgcc gtgcggatga agccgggggc cacgcagttc
     1321 accgtcacgt tgcggctggc cagttcctgc gccaggctct tgctggcgcc gacaaggccg
     1381 gccttggccg cggcatagtt cacctggccc ggattgccgg tggcgcccac cacggatgtg
     1441 atgttgacga tgcggccgaa gcgggccttc atcatcggct tggtggcagc gcgcatcagg
     1501 cggaaggcgc cttcgaggtt gaggcggatc accgcttccc attcctcatc cttcatccgc
     1561 atggccagat tgtcacgcgt gatgccggca ttgttgacga ggatatccag cttgcccagc
     1621 ttttcgagcg ctgccggcac cagcgcttcc acgctgtcct tgtcggccag attggccacc
     1681 acgcacacat gatcaccgcc cagctcggcg gcaaaggcct ccagcttgtc ggcattgctg
     1741 cccgagagcg ccaggcgggc gccctgcccg gccagtgccc tggccaccgc gctgccgatg
     1801 ccccccgatg cgcccgtgac gagcgcggtc atcccttcca gcgaaaacat catgcgatct
     1861 ccttcagcag cgcctcgatg tcggccatcg tcaccacgct ggtcactttc acatccttca
     1921 cgctgcggcc gatcatcggc cccagcacct tgccgcccag ctcgacgaat tcggcaacgc
     1981 cggccgcctg catggcgatc acgctctcgc gccagcgcac gcggccacac acctgctcca
     2041 ccagcaggcg ctgcactacg gccgggtctg tcaccacatc ggcggtgaca ttggcataga
     2101 gcgccacgcg cagggcgccg ggcgccgttt tcgccagcgc ctcggccatg gcatcggcgg
     2161 cgggctgcat cagcgggcag tggaacgggg ccgacaccgg cagcgccacg ccgcgcttga
     2221 tgccatgctc cttcaccagc gcgatggccc gctcgatcgc gcccctgtgg cccgaaagga
     2281 caacctggcc gggatcattg tcgttggcca cggcgcacac ttcgctggtg ccatcaggcg
     2341 ccacggcggc ggcctgcgcc agcgcggtgg ccttgtcgat atcggcgccc agcagcgctg
     2401 ccatcgcccc cacgcccacc ggcacggcgg cctgcatggc ctgcccgcgc agcttcagca
     2461 gccgcgccgt atcggccagc gagaaagcgc cggcagcgca cagcgcggta tattcgccca
     2521 ggctgtgacc ggccacgaaa tcggccttgt cggccagcgt gatgccgccc tcggcctcgg
     2581 ccacgcgcag ggtggcgatg gcattggcca tgatcgccgg ctgggcgttc tcggtcaacg
     2641 tcagctggtc ctcgggccct tcggccatga ggcgcagcag gttctgcccc agcgcctcgt
     2701 ccacttcctg gaacacctcg cgggcaatgg cgctggcctc ggcaagctct acgcccatgc
     2761 cgaccttctg gctgccctgc cccggaaaca caaatgcgcg cataaatcgc tagtccctta
     2821 cagtctggcg cgcgggccta ttgccttacg cgcgccggcg caaggccgaa gggcacgatc
     2881 cggttatccg catcatggcc gatatcggcc gcgcccagaa aggggatggc atggcgcgcg
     2941 caccagtcct gcgcgatggc ctcggcactg gcgccaaacg ggcggtcgtt ctcgggcaca
     3001 tcggaaaccc ggcccagccg caggccggca atccctgcaa gatgggccgt ggcatggaag
     3061 aacagcctgt ccaccgcgta gagatattcg ctcacttcct ccaccatcac cacatggcca
     3121 gccaggcccg gcatcagcgg cgtgccgacc agcatcgcca gcgtggtgag gttgaaggcc
     3181 acggcaggat gggtgctgcc ggccagcgag ggctccagcc ccccgccctc gcccttcagg
     3241 aaggccagtg cgcgggccag cgctgccgcc ccctcctgcc gccgcccgtc ggccagcatt
     3301 ggcccatggc agggccggcc gatgcggtgg cgatagagcg cgcccaaaag atagcccatg
     3361 tcggaatagc cgaggaagac cttgcccgcc gctgccgccc ccagccgcgc caccgccgcg
     3421 ccggcaatgc ggttgctgcc atagccgccg cgggcaaacc acacggcatc cgtgcccgga
     3481 tcatgggcgc aatcgaggaa ggcttccagc cgctgcgcgt cgctgccggc aaaatggccc
     3541 tcggccagga agcattgcgg gtgaacgttc agctccaccc cgcccacgct atcggccacg
     3601 gcctgggcac gggccacatc ctcgggcgtg atcggcgtgg cgggggcgca gatggcaatg
     3661 cggatcgtca tggcgcctgc aatagcccgt tgcgcccatc cccgccaccg cctaggcgtg
     3721 gccgccatgg cagacagcgc gctcacttcg catccgtggt ttttctgtgg catcggcggc
     3781 tccggcatgt tgccgctggc gctgatcctg aaggggctgg gggccgaggt ggccggctct
     3841 gaccgggggc gcgatcaggg gctgacgccg gagaaattcg cctggctgga agggctgggc
     3901 ctgcgcctgt tcccgcagga tggcagcggc cttgcggcgc ccgatcaggt gctggtggcc
     3961 tctgccgccg tggaagacag cgtgcccgaa gtggtgcgcg cgcagcaact gggctgcccg
     4021 cgcatgagcc gggccgaact gctggcccgc ctgttcaacg ccgcgcccgc ccgcatcgcc
     4081 atcggcggca ccagcggcaa gagcacggta accggcatga ccggctggat cctcaccgcc
     4141 acgggccgcg accccactat catgaacggc gcggtgatga agaatttcgc cacgccagag
     4201 gccccctttg ccagcgcgcg ggtgggccag ggcagcctgt tcgtctctga agtggacgag
     4261 agcgatggct ccatcgcgct ctatcagcca acggtggcgg tgctctccaa tgtcagcctc
     4321 gatcacaaga gcctggagga attgcgcgcc ctgttcggtg cctttctggc gcgggcggat
     4381 atcggtgtcg tcaatctcga caatgccgag gccgccgccc ttgccccgca ggcgcgccgc
     4441 ctcgtcagct ttggcataga cagcgctgcg gccgatcttg gcgtggtgcc cggcagcatc
     4501 gttgaagggg caacgcagat cgccgccacc cttgtcgacc ggcgctgcgg cgctactcat
     4561 gcgctggcct tgcgcgtgcc gggccgccac aatctggcca atgcgctggc cgcccttgcc
     4621 gcggccagcg cagccggcgt gccgctggca gaagcggtgg cagcgctggg cgcctatgcc
     4681 ggccttgccc ggcggttcga cattgtcggc accagcccat ccggcatcac cgtgatcgac
     4741 gatttcggcc acaaccccga caaggtcgcc gccaccctgg ccacgctgaa agcgcatgaa
     4801 ggtcgcgtga tcgccttctt ccagccgcat ggctatggcc cgctgcggca gatgggggcg
     4861 gaactggccg ccactttcgc gcgtcttttg gggccggggg atgaagcgat cctgtgcgat
     4921 ccggtctatt tcggcggcac ggtcgatcgc agcctgggcg cagagcgcat tgtcgcgctg
     4981 atcggcgagg ccggcggcca tgcccggcac atccccgcgc gggccgatgt ggccacccat
     5041 atcgccgccg tggcccggcc tggcgatcgc atcgtggtga tgggcgcgcg tgacgacagc
     5101 ctcagccgct ttgcccgcga gctgctggcc gccctgccct gagcgccgaa cacacgcatg
     5161 ccggcctgcc cccgcccaac gccaggaaca ggccgacacc gtttgcaaaa cgccctgcgg
     5221 cgttaaatcg cccgcagctg caccagcgtc tggttcacca gctgataggc gatctcgccg
     5281 aaagtgacag ggccggccac gccgccgatc gccttgcctt ccagctcgcc atagaggaag
     5341 ttgaggatgc agttgcacga ccacagcgcg ccctcatgcg gggttgcctc cagctgatcg
     5401 cggaaagcgg ccgcataatc ggccaccggc cgggcaaagg catagttgat gcccgggaac
     5461 accggcgcat agaggctgac actgtcgccc tccaccgcgc ggatcgaggc attgacgcgc
     5521 gcgccggcat aatcgcccac cagcggcagg cggccatcgg caaggccgcg gccccgcaca
     5581 taatcggcaa acagctggct ctggccgttc accaccacct gcgcgggggc aaagccggct
     5641 tcctcgaagc ggatgacatc cacgccatcg gcctcgaaca ggttgacgat ctctacctgc
     5701 ggcagatatt cctcgggcag ttccacatgc atcaccgcca cgcggttggc atgggcggtg
     5761 ccggtcaggc catcatagac cagcgcatgg ccgcccgcct cggccagatc gaagccggcg
     5821 atccagccca cggtgggctt gaggaaggcc tgcggatagg tcgacgcctc ccgcgcgaaa
     5881 cgttcgtgca cggccgactg gaacgggatg atcgccagcg caaagccgtt ttccggcgct
     5941 tcctcgctga tgaggtgcag gctgtcggca tcataggtgc caacgctgac gccgcccagg
     6001 cccgacaggt cggtcacaaa gacgcgcgtc gcatcgatca cctggccgcc ctcggcggtc
     6061 atgaaatagg gggtggtgcc ggcaatccag cggccctggg gcagctgggc cagcgcttcg
     6121 gggcggccgg caatggccag cggcgcgccg gcctggatca gcgccacggc atcggccagc
     6181 gccaccagcc cgttcaggcg gccggcgtca cagagaggca agctgagcga tttcattgcc
     6241 gagacccttt tcccatttgg tgaagaagtt gaagaattcg ccggccttgt cggccttcga
     6301 catcgggctc agttccagcc gctgggccag catctgcacg ccaagattag tggttttcag
     6361 tcgcttaacg ccgccgtcgg ccagctttag ccaacagggg gcactcttgc tcggaagcgc
     6421 catgggcctt tcatcctcca ggtgaaaacc ggcgcgtgcc ggcaaccgcg ctgttctagc
     6481 gcggcaggcc ttgcccccgg gttcgcgcaa gtcctcgtag aaacgcgcaa gtctgcgagg
     6541 ctggaaacgc gccagtctgc gcgccgccct taaaccccgt tgcgccgcgc cgttcatcac
     6601 tgcggcaagc attcaaccgg ggagaactta catgttgctg aattccatgg ccgatgcagt
     6661 ggtctatgtg ccccgggccg aaagggtcag cttcgacatg ctggtgcgct atcgccttgc
     6721 ggccatgcgc gccacggtgt ggctgaagaa cctcacttgc ggcggcgccc ggatcgaggg
     6781 gatggcgctg gccaccggcg atgtgctggc gctctacctg ccctcactca agcccaagaa
     6841 cgcccgggtg gcctggacag tgggcaccag cgccggcctc gaattcgaac gcccccttca
     6901 ccccgacatc ttcgaaagcc tggtgctgcg ccacgcccgc ccgcgcgaac gcaccgaaac
     6961 cgatcgcctg ctggcgctgg atggcggccc ggccctgccg gcagcagcgc ttgttcccgc
     7021 ataacgcctg gccctgcgca actttcggct taacgcccgc tgcctggggc gggtggatcg
     7081 gcgtttcagc gcggcctgcg taatatttcc ttaattttgc aaatatccgc cataaccatc
     7141 gcataattat tatctcgaaa tttgataaac cccaatttac gcaagattaa gtatgtttac
     7201 ttaatcaaat ttatgggggc gccactcaca acgctcccag acatcggttg aggggtttac
     7261 catgccacgc cactttactt tcgctgccac tctggcactg ctctcgctgc cgcttgccgc
     7321 ctgttcggcc ggcaatgtcc acaccaaccg cggtttcgcc gccctcagca agacgcatta
     7381 cgccgatgcc gagagcgagc tgaagctggc cctgcaggac aatcccaacg atcccttcgc
     7441 gctgctgaac atgggcgcgg tgctgcagaa caccggccgc gggcagcagg ccgtgcccta
     7501 ttacctgcgc gtgcaggccg aggccaagca tgtctacccg cagcgcacct cgggcgacac
     7561 ttcggcgcgc cactcgctgg ccgaaatcgc cacccagaac ctgaccaagc tggccgccaa
     7621 cgacagctcg atccgcgtca ccgcgaactg agccataagg ccacacaaca aaccccgcgc
     7681 gccggctaga ccagcgcgcg gggtttgtcg tttctgcggc ccgcgcctag cggaacgcgg
     7741 gctcgttgaa ggcgcgcagc ttgcggctgt gcaggcgcgg cccttcggcg cgcagcaatt
     7801 cgcaagtggt ggtgccaatc ttgaggtgag cggcaatcgc ctcctcgtag aagcggttgg
     7861 cctggccggg cagcttgatc tcgccatgca gcggcttgtc cgacacgcac agcaaggtgc
     7921 catagggcac gcggaagcga tagccctgcg cggcgatcgt cgcgctttcc atatcaatcc
     7981 ccaccgcccg gctgagcgac agccgccggg ccgaggcggt atagcgcagc tcccagttgc
     8041 ggtcgtcggt cgtcaccacc gtgccggtgc gcatgcgctt cttgaggttg gcgccggtct
     8101 ccccgctcac cagttcggcg gcatgggcca gcgccagctg cacctcggca atggcgggaa
     8161 tggggatttc cggcggcagc accggatcca gcacatgatc atcgcgcaga taggcatggg
     8221 ccagcacata atcgccgatc cgctgcgtat cgcgcaggcc cccgcaatgg ccgatcatca
     8281 gccaggcctc cgggcgcatc accgccagat ggtcgcagat cgtcttggcg ttggacgggc
     8341 cgacaccgat gttgaccagg ctgatcccgc cccggtccgc ccgggtgagg tgataggcgg
     8401 gcatctggtg gcgccgccag gcggtatccg acagcaaagc gcgagcgtta tccacaggct
     8461 tttccaccag caatcccccg gcgcccgaca gcttttcata gccgttttgc cccagttgcc
     8521 caccggccca atcgacgaat tcatcgacat agcggtgata gttggtgaac aggataaagc
     8581 gctggaaatg ttcaggattg gtgccggtgt aatgggccag gcgggccagc gagaaatccg
     8641 tgcgcgggcc atcgaacagc gatagcggcc gcgcttcgcc aggcagaaac gcgccatcgg
     8701 ccagctcgtc gccaatctcg gccagatcgg tagtggggaa atgctgcgcc agatcgatcg
     8761 gccgcgccga aatgcccgcg gccgcatcca gcacataggg gaagggaatg ggctgggcag
     8821 accggcccac cgtcacgtcc acctcgtaat cggccatcag cagatccagc tgctcggcca
     8881 gataatcggc aaacagcgcc gggcgggtga tcgtggtggc ataggcgccg gcatgcacca
     8941 gccggccaaa ggcgcgcgca taatccgggc ggatcacccc gccggcatag ctgatgcgca
     9001 attccggata gcaccaggcg cgttccagat ggcgatcggg cggcggcagc gtgccatggt
     9061 cggcaaaggc ggcgatatcg gcgcgcagcg tggccaccgc ggcttcataa agggtgacga
     9121 gttcggcgat gatcggtcgg gaaaggctca tggcagcgat cctagcgcgg ccctgcggcg
     9181 ctggaaaggc ccgccccaaa ggaaaagggc gcctttccct tgcggaaaaa gcgccctttt
     9241 tctgtccgtt gccggaaagc tgatcggcag gatcagccct cggcgccctc ggcgggctcg
     9301 tccagcagct cggccgggat ctcgccgggt tcggcattgg ggtcataggc ttcggtgaag
     9361 ccgccaacct cgttgtcgaa cagcgcgtcg atcacgttga cgccctgggt ctgcagctcg
     9421 gcctcgtcgg gcgagcgagc aacgttcacc ttgaccatca ccgacacttc cgggtgcagc
     9481 gcaacgcgca cgtcgaagat gccgatggtc ttgatcgggc gctccagcac gatcatcgcc
     9541 ttggtcacct tggcgccttc ggtgttcagc gcatcggcga tatcgcgcac cgacaccgaa
     9601 ccatacagct ggccggcgtt ggaagcagcg cggatcagca ccgcctgctt gccatcaacc
     9661 gaaccggcct cgccctgggc ttcctcgcgg cgggccgcat tgcgggcctc gatctcggcg
     9721 cgctgggctt cgaacacctt gcggttcgcc tcattggcgc gcagggcctt cttgttgggc
     9781 agcaggaagt tgcgggcgta gccatccttc acggtgacaa cgtcgccaat cgtgcccagc
     9841 ttctcgatac gttcgaggag aatgatctgc atgtctcttc tcccttactt cacgaggtag
     9901 ggcagcaggc ccacgtggcg ggcgcgcttg atggcctggc tcagctcacg ctgcttcttg
     9961 gccgaaacgg cggtgatgcg cgaaggcacg atcttgccgc gttccgacat gaagccctgg
    10021 agcaggcgca cgtccttgaa gtcgatcttg ggagcgttct tgcccgagaa cgggcagctc
    10081 ttgcggcggc ggaaaaatgc acgagccatg gatcaagcct cctcgcgttc gcggcgacgg
    10141 ctgcgctcgc ggtcgttctt gcgcatctgc accgacgggc cggtttccag ctcgtcgacc
    10201 cgcacggtga tgtagcggat gatgtcttcg ttgatgccga tctggcgctc cagctcggca
    10261 acgacgccgg caggggcttc cacgttcagc atcacaaagt gcgccttgcg gttgcgcttg
    10321 atcttgtagg cgaggttctt gaggccccag gtctcggtct tggtgacctt gcccccgttg
    10381 gcttcgatga tctcggtggc ggtagcggcc agagcatcaa cctggctctg gctgagatcc
    10441 tggcgcgcca ggaacacgtg ctcgtaaagc ggcacggcag catccttcat ctgttgtaac
    10501 cgatcgctgg cttgtccgca ccattgcggg gcccctccgg ccttcgtcgc ggcagtcttg
    10561 ggcggggggc gcgaagcccc gcttgccatg aaggggcggc ctttagcgcg ctggcgccaa
    10621 aaggcaagcc cccaatcgcg ttcaggccgg cttttcagcc agaaaggcca gcagcgcatc
    10681 gcccagttcg ggccgggtca cgctgctcat atgggtgccg ggcacttcca catagcgggc
    10741 atgcaccagc agccgggcaa gcctgggcgc cgagccattg tcgagatcct catcgccgca
    10801 gaccaccagc accggctgct tcagcgcgat cagatcgtcc ggctcggtat cgtcgaccga
    10861 atgcagcagc aggcgggcgg ccaccagatc gatcttctgc gccttcatga actgttgcgc
    10921 cacataggcc ggatcgccat gcttgatggt gccatagcgg tcgatcgcat cgatgaagaa
    10981 ggcgctgcgc cgcgcccagc cggccagccc ttccagcccc atgccgccca ggatcagccg
    11041 cgccggctgc aacccggcca gaacagagcg caccgaagtg cgcgcgccca gcgaaaagcc
    11101 gcccagatca aacccgcccg gcgccagccc cagctgcggc gccagcgcca gcacatcctt
    11161 caccagcaca tcttccggcc aggcttcggg cgcatgcggg gcagcgctgc ggccatggcc
    11221 gcgcagatcc ggcatgatca cccggaagcc cgccgccgcc agcttgccgg catggccata
    11281 tttcacccaa ttcacctcgg cggtggaaaa caggccatgc agcaacagca ccggccgccc
    11341 ttctgccggc cccgcctcat gcacggccag cagcgcgcca tcgaaaccgg ggagaaagcg
    11401 gctcgtcact tcaagcgtca tggttcatcc tattcattgc ccagaaggcc gcccaacagg
    11461 ccgcccatca ccgcgcccgc cgcgcccgcg ccgatgcggc ccgaagtgtc gccgccctcg
    11521 cgcggcaggt aacggccgat ttcctcggcc aggttgatga tcggcatgga ttgcagccac
    11581 acccggccag gcccggtgag cgtggccagg aacagcccct cgccgccgaa gagaatgttg
    11641 cgaaagccgc gcagcatgcg gatatccacc tgcacggtag gatcctgcat gccgatgtgg
    11701 ccggcatgca catgcagccg ctcgcccggc gccagattgc gctccaccac ctcgccagac
    11761 aggtcgagga agaccacgcc cggccccgtc accttctgga ggatgaagcc ttcgccggca
    11821 aagaagcccg cgcccagccg ctgctgaaag aaaatgtcga gcgcaaccga accctgcgcg
    11881 cacaggaagg tttccttgcg gcagatcagc gcctggcccg gcgccagttc caccggcagg
    11941 atctgcccgg gaaagcgcgg cgcaaaggcg atgtggctgt cggtctcggc ggtgaattcg
    12001 gtgacgaaca ggccgctgcc ggaaaacatg cgcttgaggc ccgccatcaa cccgccgccg
    12061 gtgtgggtgt ccatccgcac gccgtcggtc atccaggcca tggtggcgct ctggctgtag
    12121 aggctgtcgc cgcgggccag atcgatggcc acggtctgca tggtggtgcc gtgaagttga
    12181 tagcgcatgt gtgccccctt gccgggccct tgccaatgcc ggggcccttc ccgccagcgc
    12241 ctgtgccaca gcgcccccta agggcggatg aacaggcgcc ggcgaaggcg gggcaggaga
    12301 aggcggcgcc ctcccctgcc cgcaggctgt gtcagccctt cttgggtgct ggcgtcagcc
    12361 cttcttcagg tgccggcggc ccagcagttc ggcgatctgc accgcgttga gcgccgcgcc
    12421 cttgcgcagg ttatccgaca cgcaccacag cgccaggccg ttctccacgg tggaatcctc
    12481 gcgcacgcgg ctgacgaaag tggcataatc gcccacgcat tccaccggcg tcacgtagcc
    12541 agcatcctcg cgcttgtcga cgagcatgat gcccggcgcc tcgcgcagga tatcctgggc
    12601 ctcttcggcc gagatctcgt tctcgaactc gatgttcagc gcctcggaat ggccgatgaa
    12661 caccggcacg cgcacgcagg tcgcggtcag cttgatcttg gggtcgagga tcttcttggt
    12721 ctcggccacc atcttccact cttccttggt gaagccatca tccaggaaga catcgatatg
    12781 cgggatcacg ttgaaggcga tctgcttggt gaacttcttg ggctccaccg gatcgcccac
    12841 gaagatcgcg cggctctgct cgaacagctc gtccatgcct tccttgccgg cgcccgaaac
    12901 ggactggtag gtggccacga ccacgcgctt gatcttggcc ttgtcgtgca gcggcttcag
    12961 ggccaccacc atctgcgcgg tggagcagtt ggggttggcg atgatgttct tggccttgta
    13021 gccgtcgatc gcctcggggt tcacctcggg cacgatcagc ggcacgtccg gatccatgcg
    13081 atagagcgag gaattgtcga tcaccacgca gccggcagcg gccgccttgg gcgcatagat
    13141 cttcgtcggg ccggagccgg ccgaaaacag cgcgatatcc cagccggtga agtcgaaatg
    13201 ctcgatgttc tgcaccttga gcttcttgcc ggtctcgccg aagtcgatct cggtgccctg
    13261 cgagcggggg ctggccaccg cggcgatctc gtcgatcggg aattcccgct cggccaggat
    13321 gttcagcatt tcgcggccca cattgcccgt ggcgcccacg acgactaccc gataacccat
    13381 cgtttctact ccataggccc cggcaaaggg gccgccttgg cgcggccgag acgcatgccg
    13441 catcactcac atttggcgtt ttacggcggc gtcactccat gccgtgccag aaagcggaag
    13501 atcgtgttcc agaggtgctc ggaaacgccg ggccctccta ctccatgcgc ctgcccggga
    13561 taaagcatca tctcgaaggc ttgcgcattt gcctgcagcc gggcgatcgc ctcggcgcta
    13621 tgggcaaaca ccacattgtc gtcggccagg ccgtggatca gcagcagcgg atcggcaagc
    13681 gctgtcagcc cgtccagcgc gttggcgcgg tcatacacct cgggcaccag gcgcggatcg
    13741 cccagatagc gctcggtgta atgggtgtcg taaagctgcc agcgggtgac cggcgccccg
    13801 gcaatgccgg cggcatagag cccctgatcg gccgccagca tcttgagcgt gagatagccg
    13861 ccataagacc agccataggt ggcgatcttg cccggatcga caaagggtag cccctgcaga
    13921 tagcgcgcgc cggcccgctg gtcggccact tcgggggtgc ccatggccat gcggatggcg
    13981 ctttcaaacg ccacgccgcg gtgggcgctg ccgcgattgt cgatctggaa atggatatag
    14041 cccatggcca cgatggcctg cgccaaagcg cccgtccagc cgcgctgcac cagctgcaca
    14101 tgggggccgc cgtaatgctc gaagaacacc ggatagcgct ggcccgccac catctcgggc
    14161 aggatcatcc gccagtgcag cgtggtgcca tcctctgcct ccagcgtgcc gaattgcggc
    14221 aggcggtggg cggccctatg gggcgcatag ggatgattgc cctccacccg gttttccgcc
    14281 agccaggcca ggcgcgtgcc ctgcccgtcg gccagatagg cctgcggcgg ctgatccggc
    14341 ccgtggcgcg tgacgatcat cgcctgcccg gccgcatcca tgctggcggc atgcacgaag
    14401 ccatcctgcg tcagccgctc gggcccggcg ccgggggtgc tataatcgag cgcatagatc
    14461 tgctgggcca gcacatcatc gcgggtggcg gcgaaaaaca gccgctcgcc cgcctcgtcc
    14521 acgccggcca gatcggtcac gtcccaggcg ccatcggtca gctggcgcca ggcgccatcg
    14581 gccagcagcc acagatggcc atggccatcg cgctcgctcc accagaccag ccggccattg
    14641 gccaggaagc gataggcatc cgaaaggttg atccagtggc cgggcgcggc ttcctcggtg
    14701 aacagcaggc tggcagcgcc cgtggccgga tccaccttca gcatgtcgag ccgcccctgc
    14761 gcgcgatcct gccgctgcac atagagcgcg cggccatccg gcgcccaatc gacgcgggcg
    14821 agatagacat ccgccccctc gcccagatca acgcgcaccg catcgctgcc atcgggcgcc
    14881 atcagccaca gcgccacagc ggcattggcc gtgccggcag ccggatagcg ctggcgcacc
    14941 atggccgtgc cctcgccgcc gatgctggcg cgcagcactt ctgccacgcc gctctcgtcg
    15001 aaccgctgaa cggccaggcg gctgtcatcc ggcgcccacc aatagcccga gaggcgcgcc
    15061 atctcttcct gcgcaacaaa ttccgcctcg ccccagcgca ccgtatcggc gctttccgca
    15121 ggggttatag cagaggcgag ccggccccgg ggcgccaccc acagccgccc ttcagccaga
    15181 taggaaacat aaccgccggc agggctcagc cgcgcatcga tcgcgcccgt gccggtatcc
    15241 agccgctcca gcgcgccatc gaggcccgcc agccacaggg cgccgggcgt tggcaccacc
    15301 agcgccgtgc catcgcgcgt ccagccgaag gagaggatgc cgcgccgctc gcccaggcgc
    15361 aggcgctcgc ggcgcatctt ctccgcctcg ctcagcgccg cgccttcttc cagccgggtg
    15421 gaatccacca gcatccgcca ctggccgctg gccctttccc agccccacag atcatgccgg
    15481 ctgcgctccc cctcgcgcgg gcgcagcacc gccacccagc cgccgccggg ggcaatgcgc
    15541 ggctgcaccg gcgcgctgcc ggccagcgcc gggctggcaa acacccgcgc gaaatcgagc
    15601 gggggatggg catcggccat ggggaaaacg gcctttcgaa cgggattggg cgcagaaaaa
    15661 aggcgccccg gctgtgggcc agggcgcctt ctcgaaacgg atgatccgtc tgtcgggcgg
    15721 ggccttagcc ctcctggccg cgggtgtcgc gacgctcggc aatacgggcg cgtttgccgg
    15781 tgcggccacg caggtagtac agcttcgcac gacgcaccac gcccttgcgg acgacggcaa
    15841 tcgaatcgat gttgggcgaa tagagcggga acacacgctc cacgccttcg ccgaacgaca
    15901 tcttgcgcac ggtgaagttc gagttgatgc cacggttcga acgggcgatc accacgcctt
    15961 cataggcctg aacgcgggtg cgctcgcctt cgatcacctt cacgcccacg cgcacggtgt
    16021 cgccggggcg gaattcgggg atctccttgg cggccttggc gatttcctcg gcctcgatct
    16081 gctgaatcag gttcattgac ccaaatcctt cgtttcctgc cgcgcgccag aggcagactg
    16141 gccccgaacg ccactgtggc gctcccaaag gtccggcctg cgtaaccgtg tatcggcaag
    16201 cgcctgttgt ttccgccagg cggcgatctt gccgtgatcc cccgatcgca gcacttcggg
    16261 gatcatatgc ccttcccact cggtgggccg ggtgtagtgc ggatattcga ggaggccgct
    16321 ttcgaacgac tcctcatccc cactggaagc cgcgcccatt acgccgtcaa gcaggcgaat
    16381 gcaagcatca agcagcatca gcgcggccgg ttccccgccc gacaggacga tgtcgcccac
    16441 gctgatctct tccacctgcc ggccggcgaa aatccgctcg tcaaagccct cgaaccggcc
    16501 gcacaggatg gtcacccccg gccccgccgc cagttcgcgc acgcgcgcct gggtcagcgg
    16561 cttgccgcgc ggggtcatcg ccaccaccgg gcaatcggga tggcttgcgc gggcatgatc
    16621 gatggccttg gccagaatat ccaccttcag caccatgccg gcgccgccac cggcgggggt
    16681 gtcgtccacc gtgcggtgtt tgtcttcggc aaagctgcgg atgtgcacgg gatcgagggt
    16741 ccacttgccc tcggccagcg cgcggccggc cagagacacg ccgagcgggc ccggaaacat
    16801 ctcgggatag agcgtcagca ccgttgcggc aaaagccatg gctcagccct cttgcgcgcc
    16861 ggcgtcggca tcattgtcgg cataggcggc agcgacgatc agccgctcct cgtcccacgc
    16921 gggcaccgca tcgggccgca tcggcaccat gaagcgggca ggcttggccc ccgcggctgc
    16981 cggcgcctcg atctcgacga tatcgccggc gccgaaattc tccaccgcca ccaccacgcc
    17041 cagcgccgcg ccggcatccg acaccgcggc caggccaatg agatcggcat ggtaatattc
    17101 gccctcggcc agcggcggca gagcgctgcg cggcacggac aatagcgtgc cccgcagttt
    17161 ttccgccgcc gtccggtcgg ctatttcggc aaagcgggca atcgcgccgc ccttgccgtc
    17221 gtcacgcagg gccgtgggcg aaagcgcgcc gccattgaac gccttgtagc gtttgaggcc
    17281 ggccgcgctt tcgccaaaca gcttcaggcg cacctcgccc gccaccccat gcgcagcggc
    17341 caccgccgcc atggggatca tgcgttcggg gcgcccgcca gccattactc ggcagccgcc
    17401 tcggcagccg cctcagccgc ggcagcagcc ttctcggcct tctcctcggc gcgttccttg
    17461 gccttcttgc cgggctccgc cttgttcggg ttgttggtgg ccttgcgctc cttcacgccg
    17521 gccttgtcga gcaggcgggc aacgcggtcg gtcggctggg cgccaacgct cagccagtag
    17581 cgcacgcggt cctcgatgag gcgcacgcgg ttttcatcgt ccttggccag cagcgggttg
    17641 taggtgccca cctgctccag gaaggcacca tcgcgcgggg cggtcgaatt ggcgatgacg
    17701 atcttgtaat aggggcgctt cttggaaccg ccacgcgaca gacgcagaga aaccgacata
    17761 gttctacctt tccactctaa attcttgcga aacttacttt ttcagaaact tcatgaaatc
    17821 gggcgcgcca ccgccgccaa ggccgggcat accgcccagc ccgccgggca ggccgccacc
    17881 gggcacgccg ccggcagcgc cgccgccgcc aagaccaccc agcccgccgc cgccgccgaa
    17941 catcgcggcc aggcccttca gcccgcccat cttcttgatc tgcttcatcg cgcgctccat
    18001 ttcctggtgc atcttgatga gcttgttcac atcctgaacc tgcacgccgg cgcccttggc
    18061 gatgcggatc ttgcgcttgg cgttcagcag atcgggcttg gcgcgctcct tgggcgtcat
    18121 cgagctgacg atggcatcca tgcgcagcag cgtgcgatcg ctcatgccgc tggcatccat
    18181 cgccgccttg gccttcttca tgcccggcag catgcccgcc agcatgccca gcccacccat
    18241 cttctgcatc tggcgcagct gggtctgcag gtcggtcatg tcgaactggc ccttggccat
    18301 gcgctgggcc aggcgctcgg cctcgtcggc ctccaccgcc gcggccgcct tctccaccag
    18361 gctgacgaca tcgcccatgc ccaggatgcg cccggcaatg cggccggggc tgaacacttc
    18421 cagctggtcg agcttttcgc ccatgccggc aaacttgatc ggcttgcccg tcaccgcgcg
    18481 catcgacagc gccgcgccgc cgcgcgcatc gccatccagc cgggtgagga tcacaccggt
    18541 cagcgccacg ttctgggcaa agctctgggc aacgttcacc gcatcctggc cggccagcga
    18601 atcgacgacc agcagcgttt ccttgggcga ggcgatggcg gccaccgcct tcatctcgtc
    18661 catcagcgcg gcatcgacat ggaggcggcc ggcggtatcc agcagcagca catccaccgc
    18721 ctgcagcttc gcggaattga ccgcgcgctc ggcaatcgcc gtcggggtct ggccggcgat
    18781 gatcggcagc gtggccacat ccacctgcgt gcccagcacg gccagctgct cctgcgcggc
    18841 cgggcggttg acgtcgagcg acgccatcaa caccttcttg cccagcttct cgcgaatgaa
    18901 cttgccgagc tttgccgtgc tggtggtctt gcccgagccc tgcaggccga ccatcatgat
    18961 caccaccggc ggcgttgcgg ccagatccag gcccggcaca tcctcgccgc ccagcatcgc
    19021 caccagcgca tcgttgacga tcttgacgac ctgctggccc ggcgtgaccg acttcagcac
    19081 gttctggcca atggcctttt ccgtcacctc gtcgatgaag cggcgcacca ccggcagggc
    19141 cacatcggct tccagcaggg caatgcgcac ctcgcgcatg gcatcgcgca cgtcctgctc
    19201 cttgagcgca ccgcgcccac gcagcttgtc gaacacaccg ccaaggcgat cggacagatt
    19261 gtcgaacatg cctcacgcac tcctgccggg ccggaatacc ccaagcccat cacgccaaaa
    19321 acgccggtgg gcgaaacctc gccgaccagc gccgaaccac cggccccaaa gggccaaagc
    19381 catgatagcg aaaaacgccg gcggacgaaa cctcgtcggc cagcgtatcg cctgatgaca
    19441 agcggtacat cgttgtatcc gttggtggag cctagcggga tcgaaccgct gacctcctgc
    19501 atgccatgca ggcgctctcc cagctgagct aaggccccat accacgggta tatatatgtt
    19561 tcgaatggtg gagcctagcg ggatcgaacc gctgacctcc tgcatgccat gcaggcgctc
    19621 tcccagctga gctaaggccc cataatccat tcaaaaacca aggaaattca gtctgggaga
    19681 ctgtctttcc cgtcccgcct catcgtcggg aggcgcccca ctaggccggt ccctttgcct
    19741 tggcaagcac ttttttcatc atgccgaacc aaaagcgttt tttgccagcc ccggcgccgg
    19801 cagccggccg accaaggcgc ccgaaaagcg ccccggccgg ccggaaacgg gatcacacct
    19861 cgtcgggttc ctcgtcgtgg ccgcccacgc ccaggtcgtc gtcgccgcca aggtcaacgt
    19921 cgttgtcggg cgaatcgccg tcgtcgtcga tgtcgagatc ctcatcggcc agatcgacat
    19981 cggcctcctc ggggccctcc ttcttctgga tttcctcgta aggaatgggc tgcttcgact
    20041 tcagcaccgg ctcgggcgtc caggcatagc cgcattcgat gcaagtaacg ggatcgtcct
    20101 tgcccagatc gtaaaagcgg gtgccgcatt tcgggcaacc gtgcttggcg ccccattctg
    20161 gcttgaccat gctcttcctc gctaggggcg cctcgcgcgc gccctttctt caaccactcg
    20221 ggacataccg aatacgatag cggcggcgcc gaagcgcctc cttctcggcc gcgcgccttg
    20281 ccatagtgcg cccgcgctgt caaaagccgc gcccgagggg gcctttggcg ccccgccgtt
    20341 cccaccagat ccggaaaccc ttcaccgtga gcgcttctgc caatcccagc ccccgccgtt
    20401 tcctgcctgc aggggccctt gccggcacca tccgcgtgcc gggcgacaag tcgatctcgc
    20461 atcgctcgat catgctgggc gcgctggcgc tgggcgaaac ccgcgtgacg ggcctgctgg
    20521 aaggcgagga tgtgatgtcc accgccgctg ccatgcgcgc catgggcgcc accatcgaga
    20581 agcaggccaa tggcgaatgg gtgatccatg gcgtgggcgt gggcgggctg ctgcagccgg
    20641 aaaccgcgct cgacatgggc aattcgggca cctccacccg gctgctgatg gggctggtgg
    20701 ccagccacgc catcaccgcc accttcatcg gcgatgcctc gctttccaag cggccgatgg
    20761 gccgggtgat cacgccgctc tccgaaatgg gcgcgcagtt caccgccagc gaaggtggcc
    20821 gcctgccgct gacgatgcgc ggcgcccagc ccgccgtgcc gatcacctat cgcctgccgg
    20881 tggccagcgc ccaggtgaag agcgccgtgc tgctggccgg gctgaacacg ccgggcatca
    20941 ccacggtgat cgagccggtg cccacgcgcg atcattccga acgcatgctg cagggcttcg
    21001 gcgccgatct gtgggtggag gaagacgccg gcgagcgcgt gattcgcctc cgcggcgagg
    21061 cggaactgac cggccagacg atcgaggtgc cgggcgatcc ctcctcggcc gctttcttca
    21121 tcgtggccgc gctgatcgtg ccgggcagcg atgtgctgat cgagaatgtc ggcctcaacc
    21181 ccacccgcgc cggcatcatc accgtgctgc gccagatggg cgggcagatc gaggaagtgt
    21241 ccccgcgcat cgttggcggc gagccggtgg cggatctgcg cgtgcgccat tccgcgctga
    21301 agggcattgt ggtggatccg gccatcgtgc cttccatggt ggacgaattc ccggtgttct
    21361 tcgtcgccgc cgcgctggcc gaggggcgca cgatcaccac cggccttgag gaactgcgcg
    21421 tgaaggagag cgaccgcatc agcgccatgc atgccgccct tgccctggcg ggcgccgcca
    21481 ccgaggagac cgcagatggc ctcatcatcg atggcaccgg gggcgcgcct ttgcgcggcg
    21541 gggccaacac gccggtcgtc acccatctcg atcaccgcat cgccatgagc atggccattg
    21601 ccggccttgt cagcaccgcc ggcgtggaag tggatgatac cggccccatc gccaccagct
    21661 tccccaattt cctggccctg ctggccacgt tgacgggcgc ctgagccatg gccctggttc
    21721 ccgccatccc cgaagcgctg gccacggccg ccggctttgc cggcatggcc ctgtgcgtgg
    21781 gcgcctatgc ctataccacg gcctacaccg gccggccgcg cgggcccaac ccctatctcc
    21841 agcatggcgg caatctggcc ggcgcgctgc tgctggtggt ctcgctgctg gtggatacca
    21901 accccgcctc gctggtgctg gaaagcgtgt gggcgctgat cgccgccacc gggctgctgc
    21961 gcgcgctgat ggcgggcaag gctgcgcaaa ggagcggggc atgatcatcg ccgtcgatgg
    22021 gcccaccgct tccggcaagg gcacgatcgc caaggcgctg gccgcccgtt tcggcctgcc
    22081 gcatctggat acggggctgc tctatcgcgc cgtgggccgg caggtggcgc tgcagggcgg
    22141 agatccggat tcacccgtag atgcgctggc cgcctgcggc tttgccgatg ccctgctggc
    22201 cgacccggaa ctgcgcagcg aggccagcgg cggcctggcc tcgcgcgtgt cggtacacca
    22261 cgatgtgcgc gcggcgctgt atgaacgcca gcgcgccttt gccacccagg ccggcggcgc
    22321 cgtgctcgac gggcgggata tcggcacggt gatcgcgccc gaggccgagg tgaagctgtt
    22381 cgtcatcgcc agcgtggctg cccgggcaga gcggcgccat gccgaaatgc tggcgcaggg
    22441 gcgcgatgtg gcgctggccg ccatcgccgc cgaccttgag gcgcgcgacc ggcgcgatgc
    22501 cacccgcgcc gaagccccct tgcgcgccgc ggttgatgcg atcaccctcg acacttccac
    22561 actgggccgc gaggaagcga ttgccgctgc cattgccgcc gttcgccagc gctgcccggc
    22621 ctgacggcgg agatcaacgc cactcgccgc aaaatccttg cttttggcgg gccattgccc
    22681 taggccgcgc ccgttccgaa cggggctgcg gccctgccag gaatgctgcg gggcgcatcc
    22741 ggcccccgcg gcggttcggc cctttttggc ccgccacaaa cccttcacgg gggccgcatg
    22801 gtgcggatgg cggaggaaag accggcggag aaaaccgcct ggccggaaac atcgatagac
    22861 tgaggaatat cccatgagca ttccctcgcg cgacgatttt gccgcgcttc tggacgaatc
    22921 gctgggcggc gccgccagcg gcggctttga aggccgcgtg gtgaagggca ccgttaccgc
    22981 cattgaaaac gacaaggccg tgatcgacgt gggcctgaag agcgagggcc gcgtggccct
    23041 gcgcgaattc gccgcgcccg gccagcccca cggtctgaag gtgggcgacg aggtcgaagt
    23101 ctatgtcgac cgcgtcgaga acgccgatgg cgaagccatg ctgagccgcg accgcgcccg
    23161 ccgcgaagcc gcctgggaca agctggagag cgagttcggc gaaggcaagc gcgttgaagg
    23221 cgtgatcttc ggccgcgtga agggcggctt cacggttgac ctcgacggcg ccgtggcgtt
    23281 cctccccggc tcgcaggtcg acatccgccc cgtgcgcgac gtgaccccgc tgatggacgt
    23341 gccccagccc ttccagatcc tgaagatgga tcgccgccgc ggcaacatcg tcgtgtcgcg
    23401 ccgcgccgtg ctggaagaga cccgcgccga gcagcgttcg ggcctgatcc agaacctttc
    23461 ggaaggccag atcctcgacg gtcaggtcaa gaacatcacc gactacggcg ccttcgtgga
    23521 cctgggcggc atcgacggcc tgctgcatgt caccgacatg agctacaagc gcgtcaacca
    23581 cccgagcgaa gtgatcgcca tcggcgacac cgtgcgcgtg cagatcgtgc gcatcaacca
    23641 cgagacgcag cgcatcagcc ttggcatgaa gcagcttgag agcgatccgt gggatggcgt
    23701 ggccgccaag tatccggtcg gcgcgaagct gtcgggcgtg gtcaccaaca tcaccgaata
    23761 tggcgccttc gtcgagctgg agccgggcat cgagggcctc gtccacgtgt ccgagatgag
    23821 ctggaccaag aagaacgtcc accccggcaa gatcgtctcg acctcgcagg aagtcgaagt
    23881 gatggtgctg gaggtcgatt ccgacaagcg ccgcatcagc ctcggcctca agcaggccca
    23941 gcagaacccc tgggaagctt tcgccgagcg tcacccggtc ggctcgaccg tggaaggcga
    24001 agtcaagaac gccaccgaat tcggcctgtt catcggtctc gatggcgacg tcgacggcat
    24061 ggtccacatg tcggacatcg cctggggcat ctcgggcgag gacgcgctgg ccctgcaccg
    24121 caagggcgag caggtttcgg ccatcgtgct tgacgttgat gtcgagaagg agcgcatctc
    24181 gctcggcatg aagcagctgg agaagggcgc gcccaccggc ggcaactcgg ccggcagcaa
    24241 cctgcgccgc aacgaggttg tcaccgtgac cgtgctggaa gtgcgcgatg gcggcatcga
    24301 ggtgcaggcc ggcgaagatg gtgccaccgg cttcatcaag cgttcggacc tgggccgcga
    24361 ccgcgacgag cagcgccccg accgcttcca ggttggccag aagatcgacg cgatggtgac
    24421 cggcttcgac cgttcgaaga agcccacctt ctcgatcaag gcccgccagc tgcacgagga
    24481 aaaggaagcc gtggaacagt atggttcctc ggatgccggc gcctcgctgg gcgacatcct
    24541 gggcgccgcg ctgaaggcca gcaagaactg agcctgtcag cctgacgcct gaacgaaagg
    24601 cccgccggaa gcgatcttcc ggcgggcttt ttcgtgcgcc cggcacagct ggccgcaagc
    24661 cggctttgcg cgcgggcgct ttcatgcgct aaagggggcc gattttgcaa aggcttgccg
    24721 tatcatgccc cttctcatcc accagttccc ctgcctggcc gacaattacg gcttcctcgt
    24781 ccacgatccc gccagcggcg agacggtggc gatcgacacg cccgatgcag aggaatatct
    24841 gcgccaggcc gaagcgcagg gctgggccat cacccagatc tggaacaccc actggcaccg
    24901 cgatcatgcc ggcggcaatc tggcgatcaa ggccgccacc ggcgcgcgca ttaccgcgcc
    24961 cgcgggcgat gccgcgaaga tcgaaggcgt ggaccgtgtc gtcgatcagg gcgacagcgt
    25021 ggcgatcggc gcctgcaccg gccatgtgct gaatgtgggc gggcacacgc tgggccatgt
    25081 cgcctatcac ctgccgggcg cggcggtgga tggcggtgat atcgcctttg tcggcgatgc
    25141 gctgttcgcg ctgggctgcg ggcggatgtt cgagggcgag gcggcgcagt tctgggccag
    25201 cctgcagcgg ctgaaagccc tgcccggcgc cacctggctc tattgcgcgc atgaatatac
    25261 cgccagcaac gcccgcttcg cccgccatgc cgatcccgac aacgccgccc ttgccactta
    25321 tgccgccgag gtggacggca agcgcgccga tggcctgcca accgtgccct tccccctcgc
    25381 ccgcgagctg gtgaccaacc ccttcctgcg cgccgatgat ccggccctgc aggcgcactg
    25441 gggcggcggc gatgcggtgg ccacctttgc cgccctgcgt gaggcgaaga acgggttttg
    25501 aggagaggat acgagggtta acaccctcgc gccataaaac ctccggcggt gcaaggctgc
    25561 gtgacgccga tacattggcg cgcacatcaa caatatcggg agcgcgaggg tgttaaccct
    25621 cgcatccctc cccttccccc tgacaacaaa aagccccgca caatggcggg gcttctgttc
    25681 tgtccggcaa ggaaagcagg atcagaggcg agcgatcgcc tcgtccacct gcgccttgtc
    25741 ctgggcgacg ccatagccgg cggcgatcag gctgcgggcg gcaccagcgg cggcctgggc
    25801 cgtcttctgc tgcagctcgg ccagcgcggc gcgttcggcc gcctcgatct tgtcggcggc
    25861 gatcttctcg cggcgggcga tcacctcggt ggtgctcgct tcggccttgg cgatgatcgc
    25921 ctcggcttcc gatttggcat gggccagcat ttcggccgca tccttttcgg cattggcgat
    25981 gcggcgggca tattgcgcgc gcagctcttc ggcttcggcg cgcagggcct tggcttcatc
    26041 aagctgcttg cggatctcgg cgatcttgcc gtcgaggccc ttggtgatca cgcccggcac
    26101 cttggccttc agcgccacca gcagcagcac gaccatcgcc agcgccaccc aggcgcccgg
    26161 ggccaggccc agcgccgaag gttcggccgc ttcgccggca ttggcgccgg cagccagcag
    26221 gaacaggctt gcagcatcag ccatggagga tctccttgac ggcggcgcgg gcctgaccgg
    26281 catcgacaga gaggccggca atcttgctgg caatgtcgct ggcggcttcg gcggccacgg
    26341 tttcaatctc gcccagcgcg gcagcgcggg catgggcgat gcgggcatcg gcttcggcaa
    26401 tgcggccatc gaccttgccg gcggcctctg ccaggcgcgc ttcggtggca agagcggcat
    26461 cgtgcttggc cttggcgatc accgcctggg cttcggcacg ctggcgcgca ccatcgcgct
    26521 gcgagtggac ttcgtcctgc tcggcagcga tgcgggcggc ttcggcggcg gccagatcct
    26581 cggcgatgcg cgcctcacgc gaggcgacgg tggccatcac cttgggcacc ataccgcggc
    26641 cgacaacaaa aaaggtgaaa ccgaaaaaca gcaacagcca gaagatctgg ctggcatagg
    26701 tttcggagag ctgagcgatc tgaggcatgg ctgcatccgc tgcgtttggg gcccctgggc
    26761 ggcaagctgt ggcttgcaaa ccttcagggc catggcgcgc tgggcgcgaa aaacatggga
    26821 gaaaggagcg ggcagcgggt ttgcaccacg ctgccctcgc cctcttcccg tggccctgtg
    26881 ggccagcctg gccaggccag gccgcccgca agggccaggc ggcgaattac ttcgccacga
    26941 agatcaggat catggccacc acgaaggaga gcaggcccag aagttcggcg gcagcgaagc
    27001 cgatgaacag gcggccctgc tggccatcgg cagcgcccgg gttgcgcagg gcgctttcca
    27061 ggaacgaacc gaacacgtta cccacaccga tggcggcgat gccggcaccg atcgcggcca
    27121 gaccagcgcc gatcagcttc gcgcttgcag gttccatttc cataactcct tttcctatcg
    27181 ggaaattgtt gatccggttg cccggatatc acgcattact caggtcgaaa acttgacgag
    27241 atgtgccccc gcctcaagcc gtatctcagt gcaggttttc cgcatcgttg aggtaaagcg
    27301 aggtcaggag agcgaacaca taggcctgga taccgcccac caggatctcc agcgcctgga
    27361 tggcgatcat cagcgcaaag ctgggcaggc caaccacacc gccccacagc acgcccgaat
    27421 tggcgctggt gatgacgaag ctcgacagca cttccagcag cacgtggccc gccatcatcg
    27481 ccacgaacag tcgcaggcca aggctgaagg gacgcacgag gaaggagacc agctcgatgc
    27541 agaagatcag cgggatcatc ggcagcggcg tgccatgcgg cacgaacagg ctgaagaaat
    27601 gcagcccatg cttgccaaag ccgacgatca gcacgatcgc gaaggagatg atcgccagca
    27661 cgccagtcac ggtgaaatgg ctggtgacgg tgaagggatg gatgccgacc aggctgagcg
    27721 gcagcaggcc cagcaggttg gagaagagga tgaacatgaa cagcgagaag atgtagggca
    27781 catacttctt gccggccggg ccaatattgg cggccagcag gctgtcgata aagccggtga
    27841 agctttccac cgccatctgc cagcggccgg gcaccagcgc gcgcttcatc ccgcctgcca
    27901 caaaggccca cagcgcgatg gtgacaatgg ccatccacag cgccgaattg gtaaaggcga
    27961 tattatggcc ggccagttgc caattcgagc caaggatcgg ctcgatggtg aactgctgca
    28021 tcggatcgac ggtgccttcg tggcctgcca ctgtattgcc cctctttaaa cctgactaga
    28081 acgctggcag ataatgccat tggccgcctt gcgcccccac cggggcagcc tcaccgacaa
    28141 atagcctatc cgcctgaaat tcgaatgata ttcctgaagg caacgataat tccgaggaac
    28201 atcatcacca acaaacccca aggcgccgtt ccagccagcc ggtctatcgt ccagcccacc
    28261 agcgcgccgc ccccgatccc gcccagaagt tccgcaagca ccttgctgcc cagccggtaa
    28321 ttggcatcgg tagcatggcc cttgggctgg ttgcgcgccg cctcgcgctc gcgcacggca
    28381 cgcagccgtt catcgagcgc ggccagacgc gcctcctcac tgtctggccc cccggatggc
    28441 cggctttcgt ccatgcctgc tcccgttctg gtttcatcac cgtcaaacgg acaacaagcc
    28501 aatcgcgcag ttgcccgggg cgaggagccc cttaggcggg ggtggccccc aagtcaaccg
    28561 ctggaaggcc cgccatccgc ccccggaatt cagccattgg tctagcttgc cggaaggatg
    28621 cgggggccct gtgccctgct cgaatcacgg caatggcggc gctttgcgca ttggtatgca
    28681 caaacaccgc cgccgccggc ccgcagcaac ccgccggcag cggctgacat gccccgccga
    28741 attgcttatg cataagccct aatacctatt taaccggggc ccttatcaac cccaacccca
    28801 tcgccagaga ggacgcatca ggtggccagc ccagacccgc cccaagatcc cgccggccag
    28861 cacgccctcg gcgaagaggc gcccgatctg ttcccgcgcc ttggcgaggc gccctccgcc
    28921 cgcttccagg ccgagggcgt ggcctcgccc catgccgctg gcctggccca ccccctgccc
    28981 gatcgtatcg ccgcggccgc gcatgatccc accggccacc gcgcccggct gaggaagcgc
    29041 ctgctggaag gcgggcagga tgcgctggcc gatcacgagc tgatcgaata cctgctggcc
    29101 accgccatcc cccggcgcga caccaagccg ctggcgcgca cgctgctggc ccgcttcggc
    29161 agcctggcgg gcatcttcaa tgccgatccg gcggcgctcg ccggccaccc cggcatgggc
    29221 gaaagcagcg cggcggcggt gcatgtggtg gcggtggcgg cacggcggct gggccgggcc
    29281 cgcgtgagcg aaaagccggt gctgggcagc tggcaggcgc tgatctccta tctcaccatc
    29341 gacatggccc atctgacggt ggaacgcgtg cgcgtgctct atctcgacac caagaaccgc
    29401 ctgatccgcg acgagcatgt gaccgatggc tctgtggatt cggccgagat ccacccgcgc
    29461 gaggtgatcc acacggcaat gaacctgggc gcggccgggc tgatcctggt ccacaaccac
    29521 ccctcgggca accccgagcc cagccgcgcc gatatccaga tcaccctgcg catcgccgag
    29581 gcggggcgca atctgggaat cactgtgcac gatcatgtca tcatcgggcg cgaagggcat
    29641 gtttccttgc gtgccaaggg gctgatataa caaattcgtt acaattgccg gccatcaacg
    29701 aagcgattaa ccacaactgc ctttattctg ttgcgcgata ttaaccacgg caggccaatc
    29761 aattcgccgc gaccgcgtgg catgggcgcc atttaggtaa aaaccccatt gccaaatgcg
    29821 gaatttaccc taatcctgct ctcccgggtg cagcggacca tctgagatgg ttatgagggc
    29881 aaatccgtgg ttattcgttc cggcacaagc atttccgccc agcgcaaccg caccgcggcc
    29941 gcgccccagc cccgccgcaa tgtgctgata gagggcgagc atccgatggt ggtgctggaa
    30001 gatctggccg cccgcccgcg ccccgatgcc cacatcatcg tgctggccaa cgaaaagggc
    30061 ggcgtgggca aatccaccgt ggccttccac acctgcatgg cgctgtgcga tcagggcttc
    30121 aaggtggccg cggtcgatct cgatttccgc cagcaatcgc tggcccgggt gctggccttc
    30181 cgcgaggcaa ccgcccgccg gctggggata gagctgccca gcccgcaata caccgccatg
    30241 caccacacca gtggcgcgct gctgtgccag gaactggcgc gggtgggctg gcatgcggat
    30301 tatattgtca tcgatgtggc cgggcatgat tcgcccctgg cccggcgcgc catcgccctg
    30361 gccaacacgc tggtcacgcc ggtcaacagc tcctttgtcg atctcgatct gctgggccgc
    30421 ttcaaccctt ccacgcagga actgctgggc ccgggcttct ttgccaatat ggtgaacgaa
    30481 ctgcgcgagg agcggctgcg gcgcgacatg ggcacgatcg actgggtggt gatgcagaac
    30541 cgcgtgcggc gcggcatcag caacaatcag gcgcgggtgg atggggcgct gcaatgcctg
    30601 gcgccgcgcg cgggcttccg cctgggccag gggctgacgg aacgcgttgc ctatcgcgag
    30661 ctgttcctgc tgggcctcac ccatctcgat ctgcgccata tccccgatct gtcgcacatg
    30721 cgcgcccagg cgcgggacga gatcttcggc ctgctggaag acatgggcct gccccgcccc
    30781 gatgcccccg gcgcccccgg cgcgcgcgcg ctggacgagg ccgcccatct cgcagccgaa
    30841 gcactggccg ccgcgcaggc gcaatcctcg ctggcccatt cctcgcaggg ctctaccacc
    30901 cgggcgctgg ccgcccaccg cagcgaggcc agcgcctaac ccgccagcac aggccgcgct
    30961 tgcctcttgc ctttctgccc cccacctcct agcgagcagc gcttggccat gccggcatgc
    31021 cggccgctgg cccatctggc ggcgcgcccg cccgatgctg cctgttttcc cgcgcgcccg
    31081 cgctacagac ttcggagagt ttgccatggt cccccgctac gcccgccccg cgatgaccgc
    31141 gatctgggag ccggaagccc gctatggcat ctggttcgag atcgaggccc atgccaccga
    31201 caagctgggc gagctgggcg tggtgccggc cagcgccggc aaggccctgt gggactggtg
    31261 ggcgaccggc ccgaagatcg acgtggccgc gatcgatgcc atcgaggcgg tcaccaagca
    31321 tgacgtgatc gccttcctca cctgggtggc agagcaggtg ggcgatgaag cccgcttcat
    31381 gcaccagggc atgaccagct ccgacgtgct cgacaccacg ctgaatgtgc agctggtgaa
    31441 ggccgccgat ctcctgctag aagacctcga cgcgctgctg gccgccatca agcgccgcgc
    31501 gctggagcac aagtataccc ccaccatcgg ccgcagccac ggcatccatg ccgagccgac
    31561 gacgtttggc aagaagctgg ccgaggcctt cgccgaattc acccgctgcc gcgcccgcct
    31621 ggtggccgcc cgcgcggaag tcgccacctg cgccatttcg ggcgccgttg gcacctttgc
    31681 caacatcgat cccggcgtgg aagaatatgt ggccgaaaag atgggcctcg aggtggagcc
    31741 cgtctccacc caggtgatcc cgcgcgatcg ccacgcgatg ttctttgcaa cgctgggcgt
    31801 catcgccagc tcgatcgagc gcctggcggt ggaaatccgc cacctccagc gcaccgaagt
    31861 gctggaggcg gaggaatatt tctcgcccgg ccagaagggt tcgagcgcca tgccgcacaa
    31921 gcgcaacccg gtgctgaccg agaacctgac gggccttgcc cgcatggtgc gcagcgccgt
    31981 ggtgccggcc atggaaaacg tcgccctgtg gcacgagcgc gacatctcgc actcctcggt
    32041 ggagcgtttc atcggccccg atgccaccat cacgctcgat ttcgccctcg cccgcctgac
    32101 cggcgtggtc gacaagctgc tgatctatcc ggcacgcatg cagaagaacc tggatcgcat
    32161 gggcggcctt gtccactcgc agcgcgtgct gctggcgctg acccaggccg gcatcagccg
    32221 cgaggacagc tatcgcctcg tgcagcgcaa cgccatgaag gtgtgggaga gcgacggtca
    32281 gctgagcctg ctcgaactgc tgaaggccga tccggaagtg gccgccaagg tgccgccggc
    32341 agagctggaa gagaagttca acctcgacta ccacttcaag gcggtggatc gcattttcga
    32401 gcgcgtcttc ggcgcctgac ccctggcttt ggggcaagcg gggcgctgct ttacggcaag
    32461 cgcgccccct tcccttcccc gccaaagccc cctagcatgg ccgccagggc cgtgtgaccg
    32521 gcccacccgc gcgaggaggc acgcatcatg cagtttgtgc gcaccggcat caccgttgtc
    32581 gttctggcag ccctcgtcgt cttttcgctc aacaactgga ccccggtgga agtgcggatc
    32641 tgggacgggc tggtgctcga cacgcgcctg cccgcgctga tgctgctgtc gtggctggtg
    32701 ggctggctgc cgatgtggct ggtgtccaag gcggcgcgct ggcggctgaa gcggcgcatc
    32761 gccctgctg
//
LOCUS       DASRYE010000088        19124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_216716,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000088 DASRYE010000000
VERSION     DASRYE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19124
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216716"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /gene="thrS"
                     /locus_tag="VFF98_05040"
     CDS             <1..1941
                     /gene="thrS"
                     /locus_tag="VFF98_05040"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZU63027.1"
                     /translation="PADVAAAIGPGLAKAALAARIDGELVDLTRPFTADCTLALITAK
                     DEAEALELARHDYAHVLAEAVQALFPGTQITFGPATDDGFYYDFAPAQVDGKARPFTD
                     EDLPAIEAQMRQIIAANKPLRREVWSRAQLISRWQQQGESFKAEWAAELRGDEELTVY
                     WSGDDWLDMCRGPHLPATGKLDPAAFKLTRVSGAYWRGDQKNAMLSRIYGTGWLNKKQ
                     LDAHLTRLEEAARRDHRKLGAEMDLFHLQAEAHGSVFWHPKGFRIYRELEAYMRRAID
                     GAAYREVKTPQVMDARQWEQSGHWGKYRENMFVIPDEAPNTEDEGPIVSDDAEWMALK
                     PMNCPAHVLIFRQGIKSYRDLPLRLYENGCCHRNEPHGALHGLMRVRQFTQDDAHIFC
                     REDQIVAEVQAFCELADRIYKHFGFTYSIKLALRPEKRFGTEEMWDKAEEELRDAVVR
                     AGLATAEYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQSDRVLPERLDASYIGE
                     DGEKHRPVMLHRAIFGSYERFIGILIEHFAGKLPVWLAPVQAVVATIVSDADDYAREA
                     VAQLRAAGIRVEADTRNEKINYKVREHSLAKVPFLLVVGKREAEEGTVAIRRLGEQAQ
                     QVVPLAEAIALLRAEGTAPDLV"
     gene            2102..2968
                     /locus_tag="VFF98_05045"
     CDS             2102..2968
                     /locus_tag="VFF98_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HZU63028.1"
                     /translation="MTRLELLRRRHAPARPEEATGARARSSALPQAKADMTRHQITRQ
                     RHEIRRRRLTVSDIALLGPAMRRITFQSDELHDFLSASPDDHIKLFFPAPGADKPLMR
                     DYTPRRFDTTAGTLVIDFALHEAGPATRWAEGARVGDTLDIGGPRGSAVVPDDFDWYL
                     LVGDETALPAIGRRVEELRAGVPVITAVVLNEEAGRQSFATRAAWQGLWAYRAGAPSG
                     GADDAARLIAALAGLDLPAGEGFVFIAGETEMARTLRSYVLDTLGHPREWLKAAGYWT
                     RGEADSHGRIED"
     gene            complement(2985..3137)
                     /locus_tag="VFF98_05050"
     CDS             complement(2985..3137)
                     /locus_tag="VFF98_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63029.1"
                     /translation="MQKPLAPHLATARPRAIAMRAAAHAGQEALRLCIVYGCAAALAL
                     AGLRLF"
     gene            complement(3173..4159)
                     /locus_tag="VFF98_05055"
     CDS             complement(3173..4159)
                     /locus_tag="VFF98_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZU63030.1"
                     /translation="MTQWRFPPEWHPQEWLWIGFPHDAEEWPGFLAKAQEQIAAFASA
                     VADSGQKVRLLVRDEANATRARALCSSAVAIEQRAYGDIWLRDTGPLVLTDGQGNRRA
                     QRFGFNGWGGKYLMEGDQTIGAEIAADAGLAVASADWILEGGAIDVDGAGLVAVTEQV
                     LLNPNRNPHLSRGEIEARLLADLGQQRVLWLGDGLINDHTDGHVDNLARFVAPNTLAV
                     PVPTGADDPNAAIYADTKARAEAAGVRVCPVPSPGLVERDGRVEPASYMNFAITSHLV
                     VVPVYGTAQDAAGVAAIAALFPDRQTVGIMADAVLAGGGSFHCSSQQMPTAG"
     gene            4222..6951
                     /locus_tag="VFF98_05060"
     CDS             4222..6951
                     /locus_tag="VFF98_05060"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HZU63031.1"
                     /translation="MTTLSSPPAADWAGALARARAFAPFLARALDRFPALEALLAAGD
                     GEGALAFARAAGADAADIGTALRRERLALAAALAVGDLAGAFPLARVTGELSALADRA
                     LDAAIAHAIRSRVPGAEPGGFVALALGKQGAGELNFSSDIDPILLYDPTRLPHRPREE
                     PAEAAVAIAREVVRLLAHIDGEGYVFRVDLRLRPASEISPLALPVEAALTHYESSALA
                     WERAAFIRARSAAGDVAMGEAFLAQIRPFVWRKSLDFGAIAEIGRLTRRIRAAHGKVA
                     LPGPGFDVKRGRGGIREVEFFAQVHQLIHGGRRPELRVKGTRESLDALAAAGLISAED
                     AAAMGTAYDRLRQIEHRLQMVSDQQTHALPKTMAAIDAVAQLDGLADGAALIAELTTL
                     TEAVGARYDRLIAASAPEEPGRPAAPVAGMALEEELARLGFADPPGLAARIDGWRGGA
                     IRALRSEASLAALDAIQPALLAALAQAPEPDRALTRWEQLIARLPSAVNLFRLLEARP
                     GLLGQLADVLALAPPLADALARRADLLDALIDASALDLPGDVASLAADFGRLEAGEDY
                     QRLLDRVRRKVGDARFALGVQLIEGRHDPLDISAALARVAEAAIAVLAGATIAEFEAA
                     HGRVPGGELLILGLGRLGGGRLTHASDLDLIYLFTGDFQAESDGRRPLGATLYFNRLA
                     SRISAALSVPTAEGALFEVDTRLRPQGTQGPLAVSLASFARYQRQDAWTWEHMALIRA
                     RALFGSAPARAALAAIIAEVLAAPRDPHKLAADVLEMRTRMAEHKQPRGALDVKLLRG
                     GLVDAEFLAHFLQLRGQVALLPDMGGALRALAAAGLLPADVAGAHDVMARLLVASRLL
                     APDGEVPAPAPAAVLARSCGQGDSVELQAAVLAARKVVASAWAQAFNQTIEVT"
     gene            6951..7424
                     /locus_tag="VFF98_05065"
     CDS             6951..7424
                     /locus_tag="VFF98_05065"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU63032.1"
                     /translation="MAEFPSVGDALPDIALAGPDGAVLRPADFAGRKLVLFFYPKDDT
                     PGCTTENKDFSALSAAFAAAGVALLGISKDSPAKHAKFAAKHGLSAPLASDAEEGGLS
                     DALGIWKEKSMYGRTYMGMERSTYLVDADGKIARIWNKVKVKGHAEEVLAAARSL"
     gene            7517..8287
                     /locus_tag="VFF98_05070"
     CDS             7517..8287
                     /locus_tag="VFF98_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZU63033.1"
                     /translation="MAAALGLADPRAKLMAARGVARDWRLGRLAFAFPAAMPDRPARP
                     AAPELLPANRMPKRGRGGSERARIALWHALAHIEFVAIDLALDMAGRFGAQMGPAFVG
                     DFLGVAADEALHYALIERHLGTMGSHYGALPAHDGLWEAAAATAHDVAARLAIVPMVL
                     EARGLDVTPQTLERVRAAGDETGARILTRILADEIRHVRYGTIHFQDWCESAGKAPEI
                     TWQDLVARYFRGLVKPPFNDSARAAAGLSRGYYCALAT"
     gene            8474..9169
                     /locus_tag="VFF98_05075"
     CDS             8474..9169
                     /locus_tag="VFF98_05075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZU63034.1"
                     /translation="MSAGAMLAMALASVPVAHANAANADITAPLRAAQGGQPAADNGD
                     QAFRQLFASWRAVENGSLSSTVPGFSAHAVSIPSRMPVEGVHLTSDFGMRWHPILGGR
                     REHKGVDLAGPVGTPVHATADGTVARADWFSGYGLYIQLEHGGNLETRYGHMSRLNVA
                     AGQYVHKGDVIGYIGTTGRTTGPHLHYEVRIDGVAVNPVPYMQGEGSGQLPSGADAVS
                     AVQAGPAEADGDD"
     gene            complement(9327..10223)
                     /gene="galU"
                     /locus_tag="VFF98_05080"
     CDS             complement(9327..10223)
                     /gene="galU"
                     /locus_tag="VFF98_05080"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZU63035.1"
                     /translation="MQNTKPIKPVRKAVFPVAGLGTRFLPATKAIPKEMLPIIDRPLI
                     QYAVDEAREAGIEQLIFVTGRGKTAIVEHFDTAFELESTMRERGKSLDILEPTRIQPG
                     NLVTVRQQVPMGLGHAIWCARAIIGDEPFAIFLPDELMIGKPGCMKQMVEAYNELGGN
                     FISVLEVPREEVSSYGVIAPGAKLDGFGGALTEVTGLVEKPKVEAAPSNLIISGRYIL
                     QPEVMDVLHTQGKGAGGEIQLTDAMARMIGEQPFHAVTFAGKRYDCGSKTGFVEATLA
                     LALERPDMADDVRAVAKKLLGL"
     gene            10339..11622
                     /gene="murA"
                     /locus_tag="VFF98_05085"
     CDS             10339..11622
                     /gene="murA"
                     /locus_tag="VFF98_05085"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZU63036.1"
                     /translation="MDKIIIRGGQKLAGSVPVSGAKNAALTLLPCALLTEEPLTLRNL
                     PRLADVDGFQHLMNQFGVSTAIAGARPEDFGRVMTLQATRLTSSTAPYDLVRKMRASI
                     LVLGPLLARAGEATVSLPGGCAIGNRPIDLHLKALEAFGARIELAAGYVRAIAPDGGL
                     PGGTYAFPVVSVGATENALMAAVLARGTCRLTNAAREPEIVDLCNLLLAMGAEIDGIG
                     TSDLVIRGVDRLHGATYQVMPDRIEAGSYACAAAITGGEVELRGARPEQMEATIHALR
                     EAGVHVEVRPHGLFVAADGPLRPVTLSTAPYPGFATDMQAQLMALLTRAEGTSVLTET
                     IFENRYMHVPELNRMGANITTQGRTAIVRGVPRLTGAEVMATDLRASMSLVIAGLAAE
                     GETQVHRLYHLDRGYERLEEKFALLGAEIERVGGE"
     gene            complement(11676..11972)
                     /locus_tag="VFF98_05090"
     CDS             complement(11676..11972)
                     /locus_tag="VFF98_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63037.1"
                     /translation="MRKLILALGATAVVAMSPVGISSAEARPHHAKRVYKECRKSSGT
                     TGLIAGGATGALVGHALLGGPVGIAAGAVGGAFAGRGVDRAMTAKKRCHYYTRG"
     gene            complement(12054..12320)
                     /locus_tag="VFF98_05095"
     CDS             complement(12054..12320)
                     /locus_tag="VFF98_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZU63038.1"
                     /translation="MATPYHPPVKRSVEIAGHRTSVSLEPLFWDWLRAAAEAEGLPIN
                     ALVARIDAERIAAPQPPGLAGAIRLWLAARARPAAAAALPTPPG"
     gene            complement(12432..13154)
                     /gene="phbB"
                     /locus_tag="VFF98_05100"
     CDS             complement(12432..13154)
                     /gene="phbB"
                     /locus_tag="VFF98_05100"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HZU63039.1"
                     /translation="MTRIAIVTGGTRGIGEAISLALKADGVTVIANYGGNVEKAKAFT
                     DATGIPAYRWDVGDHQATLDGIAQVAADFGPVDIVVNNAGITRDGVLQKMTFDDWNEV
                     IRINLGGCFNMAKATFPGMRERGWGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAKYGVTVNAIAPGYIDTEMVAAVPAPVLEKIVAKIPVGRLGHAEEIARAVSFL
                     CSEDAGFVTGSTMSINGGQHMY"
     gene            complement(13349..14167)
                     /locus_tag="VFF98_05105"
     CDS             complement(13349..14167)
                     /locus_tag="VFF98_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63040.1"
                     /translation="MLNRAAPQAPAEPRPAGAGRSVPVQQGGFQPALVMGAAVCLLAL
                     PSAVLAFSSRLEQAPGESSANEGLRSFAPGRVDPHLARTIASSPTGPGPAFRFTPAGL
                     STRPDRSVTVAVRVDDATARTIIVRGPAQPRPGMNAAVLRIAPAAYNLGIARGNQGFA
                     AAGNGLALGADSHRADTPDISGFYISDGTRGQASPRLTPRITLDDKDHTGRSPHPLDP
                     NGDQSVGVGGSYAITRNFDVTAGVRYSQDRDRLKPLSDGKADSQAVFVGTQFHF"
     gene            14462..14971
                     /locus_tag="VFF98_05110"
     CDS             14462..14971
                     /locus_tag="VFF98_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HZU63041.1"
                     /translation="MTAAQASGHPIFRASLRGGVLCAALLALAGCSNGHLAFKNPIHI
                     KHHRENRHKPDLPPSDVTTIGVNSFLWRASLDVLSFMPLVKADSNGGVIVTDWYVNPA
                     NPAERMKITVTILDKDLRSDALRVAASRQVQQAGGWVEAPVQAATVQKLEDVILTKAR
                     DLRRDTIIQ"
     gene            15121..17637
                     /gene="leuS"
                     /locus_tag="VFF98_05115"
     CDS             15121..17637
                     /gene="leuS"
                     /locus_tag="VFF98_05115"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZU63042.1"
                     /translation="MSERFDPATADARWQSVWDEAQSFRADDASTKPRSYVLEMFPYP
                     SGRIHIGHVRNYTMGDVLARFKRMQGFEVLHPMGWDAFGMPAENAAMEKGVHPGGWTR
                     DNIANMKAQLKRLGFALDWSREIATCEPDYYGQEQALFLDLYAAGLVYRKESAVNWDP
                     IDCTVLANEQVIDGRGWRSGALVEKRKLNQWFLKITDFADELLDGLSTLEHWPEKVKL
                     MQENWIGKSQGLQFAFQLSNGASLEVYTTRPDTIFGASFVAVAADHPVAQAIGTDEAR
                     DFIALCKQGGTTAAELETAEKLGFDTGLTARHPFTGADLPVYIANFVLMDYGTGAIMA
                     VPGHDQRDFDFATKYALPITRVVAASAEDAARPFGTEAEAGDGVLVNSGFLDGLPVAE
                     AKRAVIARAEAEGWGEGKTVWRLRDWGVSRQRYWGTPIPFVHCEVCGVVPVPKKQLPV
                     TLPEDVDFSVPGNPLDRHPTWKHVDCPQCGHAARRETDTLDTFVDSSWYFLRFASQPA
                     DRPFDPAEIARWLPVGQYIGGIEHAILHLLYARFWTRALARIGKIEVAEPFASLFTQG
                     MVTHETYSRVDAGNGQPIWYAPGEVARDGNGATLIADGAPVEIGRVIKMSKSKKNVVD
                     PDEIVAKYGADAIRWFMLSDSPPERDLPWSEAGIEGCGKFVQRLWRLFGQYDGAATGE
                     DKAVDRKAHQTIAAVAGDIESLGFNKAVARIYELTGVVEKAAPSASRSAAIKVVLLLI
                     APMMPHLAEEAFAAMGGAGLVADAAWPAVDPALLVDDEVTIAIQLNGKLRDTLVAPKG
                     AAKDRLEAMALANAKVVAALDGATPKKVIVVPDRLVNIVV"
     gene            17634..18152
                     /gene="lptE"
                     /locus_tag="VFF98_05120"
     CDS             17634..18152
                     /gene="lptE"
                     /locus_tag="VFF98_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZU63043.1"
                     /translation="MKRIAGLAALALAGASLSACGLHPLYEGGGHAAAASGLAGVQIT
                     PIQGRAGWLMSAALADRVQARGSARYRLDVKLEDKLEGLGILSNDTVSRERRTLRARY
                     QLVDLATGAILLDQTAGSDEGIDPTSSEYATVAGEQTALENLTRVVADRIVANLAVAL
                     RARAAHPPAQGQ"
     gene            18149..>19124
                     /locus_tag="VFF98_05125"
     CDS             18149..>19124
                     /locus_tag="VFF98_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZU63044.1"
                     /translation="MKASQREFAGAAPRAAQGCGVFFLCGPDEASVQDAAGLIASLLP
                     DAGERVEMTGAELRRDPVRLGDEARSSSLFGGSRHIWVRSSGEEAHDAIANLIASDVP
                     FAPVVVQAPGATDKGKTAKLLERREDALVAMFYPPDLRSVTDAVRRMADGAGLRMTSE
                     LAEQIARAAGLDTRLAQGEVTKLALYLDADPQRPRTADAAAFAEVGARTEEEGMMQLV
                     NAALGGDGPALAGELRRMAELGLNGVAVLLACERRAAQLAQMAARLGPGGDVRAFVAA
                     ERQARRIFWKDEADIARQLRIWRGRALERLAERLLGLHRALLANSQSAD"
BASE COUNT         2924 a         6330 c         6731 g         3139 t
ORIGIN      
        1 cctgccgatg tggccgccgc gatcggcccc ggccttgcca aggcggcgct ggccgcccgc
       61 atcgatggcg agctggtgga tctcacccgc cccttcacag ccgattgcac cctggcgctg
      121 atcaccgcga aggacgaggc cgaggcgctg gagctggccc gccacgatta tgcccatgtg
      181 ctggcagagg ccgtgcaggc gctgttcccc ggcacgcaga tcacctttgg cccggccacg
      241 gatgatggct tctactatga tttcgcgccc gcccaggtgg acggcaaggc gcgccccttt
      301 accgacgaag acctgcccgc catcgaggcg cagatgcgcc agatcatcgc cgccaacaag
      361 cccctgcgcc gcgaggtgtg gagccgcgcg cagctgatca gccgctggca gcagcagggc
      421 gaaagcttca aggccgaatg ggccgccgaa ctgcggggcg atgaagaact caccgtctac
      481 tggagcgggg acgactggct ggacatgtgc cgcgggcccc acctgccggc caccggcaag
      541 ctcgaccctg ccgccttcaa gctcacccgc gtgtcgggcg cctattggcg gggcgaccag
      601 aagaacgcca tgctcagccg catctacggc accggctggc tcaacaagaa gcagcttgat
      661 gcccatctga cgcggctgga agaggccgcc aggcgcgacc accgcaagct gggcgccgag
      721 atggacctgt tccacctgca ggcagaagcc catggcagcg tgttctggca ccccaagggc
      781 tttcgcatct accgcgagct ggaagcctat atgcgccgcg cgatcgatgg cgcggcctat
      841 cgggaagtga agaccccgca ggtgatggat gcccgccagt gggaacagtc gggccactgg
      901 ggcaaatacc gcgagaacat gttcgtcatc cccgacgagg cgcccaatac cgaggatgag
      961 ggccccatcg tcagcgacga tgccgagtgg atggcgctga agccgatgaa ctgcccggcc
     1021 catgtgctga tcttccgcca gggcatcaag agctatcgcg atctgccgct gcgcctctat
     1081 gagaacggct gctgccaccg caacgagccg catggcgcgc tgcatggcct gatgcgcgtg
     1141 cgccagttca cccaggatga tgcccacatc ttctgccgcg aggaccagat cgtggccgaa
     1201 gtgcaggcct tctgcgaact ggccgaccgc atctacaagc acttcggctt cacctattcg
     1261 atcaagctgg cgctgcgccc ggaaaagcgc ttcggcaccg aggagatgtg ggacaaggcc
     1321 gaggaagagc tgcgcgatgc ggtggtgcgc gcggggctgg ccacggccga atatggctgg
     1381 gaagaactgc ccggcgaggg cgccttctat gcccccaagc tggaatggca cctcaccgat
     1441 gccatcggcc gcacctggca ggtgggcacc atccagtccg atcgcgtgct gcccgagcgg
     1501 ctggatgcca gctacatcgg cgaggatggc gagaagcacc ggccggtgat gctgcaccgc
     1561 gcgatcttcg gctcctatga gcggttcatc ggcattctga tcgagcattt cgccggcaag
     1621 ctgccggtgt ggctggcgcc ggtgcaggcg gtggtggcca ccatcgtttc cgatgccgat
     1681 gattatgccc gcgaggccgt ggcgcaattg cgcgccgccg gcatccgcgt ggaagccgat
     1741 acccgcaacg agaagatcaa ctacaaggtg cgcgagcatt cgctggccaa ggtgccgttc
     1801 ctgctggttg tcggcaagcg cgaggccgaa gagggcacgg tggccatccg ccggctgggc
     1861 gagcaggcgc agcaggtggt gccgctggcc gaggccatcg ccctgctgcg ggccgagggc
     1921 acggcgcccg atctcgtttg atctgaccct tatccacgac acatgggaac ggcgcctgcg
     1981 ggcgccgttt tcgttggcgc ttgcggatct ttcgcaaaaa agcgtgagtc gctcttgcta
     2041 aataatgcac aagatatatc ttgattgcac cgagacgatt cgcaagaagg aaccgccccg
     2101 catgacccgc cttgaactgc tgcgccgccg ccatgcaccg gcccgcccgg aagaggcgac
     2161 aggggcccgc gcgcgatcgt ccgctcttcc ccaagcgaaa gccgatatga cccgccacca
     2221 gatcacccgc cagcgccatg aaatccgccg ccgccgcctc accgtcagcg atattgccct
     2281 gctgggcccg gccatgcgcc gcatcacctt ccaaagcgac gagttgcatg atttcctcag
     2341 tgcctcgccc gatgatcaca tcaagctgtt cttccccgcg cccggcgccg acaagccgct
     2401 gatgcgcgat tatacgccgc gccggttcga caccaccgcc ggcacgctgg tgatcgattt
     2461 tgccctgcat gaggcaggcc ctgccacccg ctgggccgag ggcgcccggg tgggcgacac
     2521 gctggatatc ggcggcccgc gcggttctgc cgtggtgccc gatgatttcg actggtatct
     2581 gctggtgggc gatgaaaccg cgctgcccgc cattggccgc cgggtggagg aattgcgcgc
     2641 cggcgtgccg gtgatcaccg ctgtggtgct gaacgaggag gccggccgcc agagctttgc
     2701 cacccgcgct gcttggcagg gcctgtgggc ctatcgcgcc ggcgcgccca gcggcggggc
     2761 cgatgatgcc gcccggctga tcgccgcgct ggccgggctg gatctgccgg cgggcgaggg
     2821 ctttgtcttc atcgccggcg agacggagat ggcccgcacc ctgcgcagct atgtgctgga
     2881 tacgctgggc cacccgcgcg aatggctgaa agccgccgga tactggaccc gcggcgaggc
     2941 cgattcgcac ggccgcatcg aggattgatg gaggctggcg gccctcagaa cagcctgagg
     3001 ccggccagcg ccagcgccgc cgcgcagcca tagacgatgc acaggcgcag cgcttcctgc
     3061 ccggcatggg cggcagcgcg catggcgatg gcacgggggc gggcggtggc aaggtgcggg
     3121 gcgaggggct tttgcatgat gcaaaagctg cgccccaaac cctaaggaag cgttaaccgg
     3181 ccgtgggcat ctgctggctg gagcaatgga agctgccgcc ccctgccagc accgcatcgg
     3241 ccatgatccc caccgtctgc cgatcgggga acagggcggc gatggccgcc acgcccgccg
     3301 catcctgcgc cgtgccatag accggcacga cgacgagatg gctggtgatg gcaaaattca
     3361 tgtagctggc cggctccacc cggccgtcgc gctccaccag cccgggcgag ggcaccgggc
     3421 acacgcgcac accggccgcc tcggcgcggg ctttggtgtc ggcatagatg gcagcgttag
     3481 ggtcgtcggc gccggtgggc acgggcaccg ccagcgtgtt gggcgccaca aaccgcgcca
     3541 gattgtcgac atggccgtcg gtatggtcgt tgatcagccc gtcgcccagc cacagcaccc
     3601 gctgctggcc cagatccgcc agcagccgcg cctcgatctc cccgcgtgag aggtgggggt
     3661 tgcggttggg gttcagcagc acctgctcgg tgacggcgac aagcccggcg ccatccacgt
     3721 cgatcgcccc gccttccagg atccagtcgg cgctggccac ggccaggccg gcatcggcgg
     3781 cgatctcggc gccgatcgtc tggtcgccct ccatcagata tttgccgccc cagccgttga
     3841 agccgaagcg ctgcgcccgg cggttgccct ggccatcggt cagcaccagc ggccctgtgt
     3901 cgcgcagcca gatatcgcca taggcgcgct gctcgatggc aacggcgctg ctgcacaggg
     3961 cgcgggcgcg ggtggcattg gcctcatcgc gcaccagcag gcgcaccttc tggccgctgt
     4021 cggccacggc gctggcaaaa gcggcgatct gctcctgcgc cttggccaga aagcccggcc
     4081 attcctcggc atcatgcggg aagccgatcc agagccactc ttgcgggtgc cattcggggg
     4141 ggaagcgcca ttgggtcatg gcccgcccca tagccgcgcg cgcgccttgc gggaagcgaa
     4201 agagcagcgc acaagcgggc gatgaccacg ctttcttccc cgcctgcggc cgattgggcc
     4261 ggcgccctgg cccgggctcg cgcgtttgcg ccgtttctgg cccgcgcgct cgatcgcttt
     4321 ccggcgctgg aggccctgct ggcggcgggc gatggcgagg gggcgctggc gtttgcccgg
     4381 gccgccggcg ccgatgcggc cgatatcggc acggcgctgc ggcgcgagcg gctggcgctg
     4441 gccgcggcgc tggcggtggg cgatctggcc ggtgcctttc cgctggcgcg ggtgacgggc
     4501 gaactgtcag cgctggccga cagggcgctg gatgcggcga ttgcccatgc catccgcagc
     4561 cgcgtgccgg gggcagagcc gggcggtttc gtggcgctgg cgctgggcaa gcagggggcg
     4621 ggcgagctga acttctcctc cgacatcgac cctatcctgc tgtacgatcc cacccgcctg
     4681 ccccaccgcc cgcgtgagga gccggcggag gccgcggtgg ccatcgcccg cgaggtggtg
     4741 cgcctgctgg cccatatcga tggcgagggc tatgtcttcc gggtggatct gcgcctgcgg
     4801 cccgccagcg agatcagccc gctggcgctg ccggtggaag ccgccctcac ccattacgaa
     4861 agctcggccc tggcgtggga gcgggcggct ttcatccgcg cgcgttcggc cgctggcgat
     4921 gtggcgatgg gcgaggcgtt tctggcccag atccgcccct tcgtctggcg caagagcctc
     4981 gatttcggcg ccattgccga gatcggccgg ctgacgcgcc gcatccgcgc ggcccatggc
     5041 aaggtggcgc tgccgggccc gggctttgat gtgaagcgcg ggcgcggcgg catccgcgag
     5101 gtggaattct tcgcccaggt gcaccagctg atccacggcg gccgccgccc ggaactgcgc
     5161 gtgaagggca cgcgcgaaag ccttgacgcg ctggccgcgg cgggcctgat ctcggccgag
     5221 gatgccgccg ccatgggcac cgcctatgac cgcctgcgcc agatcgagca ccgcctgcag
     5281 atggtgagcg accagcagac ccacgccctg cccaagacga tggccgcgat cgatgccgtg
     5341 gcgcagctgg atgggctggc cgatggcgcc gcgctgatcg ctgagctgac caccctcacc
     5401 gaggcggtgg gcgcgcgcta tgaccggctg atcgccgcca gcgcgcccga ggagcccggc
     5461 cgcccggccg cgccggtggc cggcatggcg ctggaggagg agctggcgcg gctgggcttt
     5521 gccgatccgc ccggccttgc cgcccgcatc gatggctggc gcggcggggc gatccgggcg
     5581 ctgcgttcgg aagcctcgct ggccgcgctc gatgccattc agccggcgct gctggcggcg
     5641 ctggcccagg cgccggaacc tgatcgcgcg cttacccgct gggagcaact gatcgcccgc
     5701 ctgcccagcg ccgtgaacct cttccgcctg ctggaggcgc ggccgggcct gctgggccag
     5761 ctggccgatg tgctggcgct ggcgccgccg ctggccgatg cgctggcccg ccgggccgat
     5821 ctgctcgacg cgctgatcga tgcgagcgcg ctggatctgc cgggcgatgt ggccagcctg
     5881 gcggcggatt ttggccggct ggaggccggc gaggattatc agcgcctgct cgacagggtg
     5941 cgccgcaagg tgggcgatgc ccgctttgcg ctgggcgtgc agctgatcga ggggcggcac
     6001 gatccgctcg atatttccgc ggcgctggcg cgcgtggcag aagcggcgat tgccgtgctg
     6061 gcgggcgcca ccatcgccga attcgaggct gcccatggcc gggtgccggg cggcgagctg
     6121 ttgattctgg gccttggccg gctgggcggc gggcggctca cccatgcctc ggatcttgat
     6181 ctcatctatc tgttcaccgg cgatttccag gccgaatcgg atggccgccg gccgctgggc
     6241 gccacgcttt atttcaaccg cctggccagc cggatcagcg cggcgctgtc tgtgcccacg
     6301 gccgaaggcg cgctgttcga ggtggacacc cgcctgcgcc cgcaaggcac gcaagggccc
     6361 ctggcggtga gcctcgccag ctttgcccgg tatcagcggc aggatgcctg gacctgggag
     6421 catatggcgc tgatccgcgc gcgggcgctg ttcggctcgg ccccggcgcg cgcggcgctg
     6481 gcggcgatca tcgccgaagt gctggccgcc ccgcgtgatc cgcacaagct ggccgcagac
     6541 gtgctggaga tgcgcacccg catggccgaa cacaagcagc cgcgcggcgc gctggatgtg
     6601 aagctgctgc gcggcgggct ggtggatgcc gaatttctgg cccatttcct gcaattgcgc
     6661 ggccaggtgg cgctgctgcc ggatatgggg ggggccttgc gggcgctggc ggcggccggg
     6721 ctgctgccgg ccgatgtggc cggcgcccat gatgtgatgg cgcggctgct ggtggcctcc
     6781 cggctgctgg cgcccgatgg cgaagtgccg gccccggcgc cggcggcggt gctggcgcgc
     6841 tcttgcgggc agggcgatag cgtggaattg caggccgcgg tgctggccgc ccgcaaggtg
     6901 gtggccagcg cctgggcgca agccttcaac cagacgatag aggtgacatg atggccgaat
     6961 ttccgagcgt gggcgatgcg ctgcccgata tcgccctggc aggcccggat ggcgccgtgc
     7021 tgcgcccggc cgattttgcc ggccgcaagc tggtgctgtt cttctacccc aaggacgaca
     7081 cccccggctg caccaccgag aacaaggatt tctccgccct gtctgccgca tttgccgcgg
     7141 ccggcgtggc gctgctgggc atttcgaagg acagccccgc caaacatgcc aaatttgccg
     7201 ccaagcacgg gctgagcgcg cccctggcct ccgatgccga ggaaggtggc ctttccgatg
     7261 cgctgggcat atggaaagag aagagcatgt atggccgcac ctacatgggc atggagcgta
     7321 gcacctatct ggtggacgcc gacgggaaga tcgcacggat ttggaacaag gtgaaagtga
     7381 agggccacgc cgaggaggtg ctggcggcag cgcgcagcct gtgaagccgg cggttggcga
     7441 tgcccctgcc gttggcgatg cccctgccgt tggcgatgcg cctgccgttg gcgatgcgcc
     7501 tgccgttggc gatgcgattg ccgctgccct gggcctggcc gatccgcgcg ccaagctgat
     7561 ggcggcgcgg ggcgtggcgc gcgattggcg gctgggccgg ctggccttcg cctttcccgc
     7621 cgccatgccc gatcgccccg cccggccggc cgcgcccgaa ctgctgccgg ccaaccgcat
     7681 gcccaagcgc gggcgcggcg gatcggagcg ggcgcgcatc gccttgtggc atgcgctggc
     7741 ccatatcgaa ttcgtggcga tcgatctggc gctggatatg gcgggccgat tcggcgcgca
     7801 gatgggcccg gccttcgtgg gtgatttcct cggcgtggcg gcggacgagg cgctgcatta
     7861 cgcgctgatc gaacgccatc ttggcacgat gggcagccat tatggtgcgc tgcccgcgca
     7921 tgatgggttg tgggaagcgg cggcggccac cgcccatgat gttgccgcgc ggctggccat
     7981 cgtgccgatg gtgctggaag cgcgcggact ggacgtaacc ccgcagacgc tggagcgcgt
     8041 gcgcgcggcc ggcgatgaaa ccggcgcgcg catcctcacc cgcatcctgg cggacgagat
     8101 ccgccatgtt cgttacggca cgattcactt tcaggactgg tgcgaaagcg ccggcaaagc
     8161 gccggaaatc acctggcagg atctggtggc gcgctatttt cgcggattgg ttaagccgcc
     8221 gttcaacgat tcagcgcgcg ctgctgccgg tttgtcgcgc gggtactatt gcgcgcttgc
     8281 aacataagcg cctccccggt tagccctgtg gcaggacgga agggggggcc tttccatcca
     8341 acgcaatatg tctctacggg ctcctctgcg ggcgtccgcc aaggtcgcat gcgtgctgtt
     8401 cctcaccggg atggttcgcc aaaaatccag gatggtatct cgcgtggtcg caatttcgat
     8461 gcttgcaagg ggaatgtccg ccggcgccat gctggcgatg gccctggcct cggttcctgt
     8521 ggcgcatgcc aacgctgcca atgccgatat caccgcgccg ctgcgcgccg cccagggcgg
     8581 ccaaccggcg gcggataatg gcgatcaggc cttccgccag ctgttcgcct cgtggcgcgc
     8641 ggtggaaaat ggctcgctca gctccaccgt gccgggtttt tcggcccatg cggtgtcgat
     8701 cccctcgcgc atgccggtgg aaggcgtgca cctcacttcc gatttcggca tgcgctggca
     8761 cccgatcctg ggcggccggc gtgagcacaa gggggttgat ctggccggcc ctgtgggcac
     8821 cccggtgcat gccacggcgg atggcacggt ggcccgggcg gattggttca gcggctatgg
     8881 cctctacatc cagctggaac atggcggcaa tctggaaacc cgctatggcc acatgtcgcg
     8941 cctcaacgtg gcggcgggcc agtatgtcca caagggcgat gtgatcggct atatcggcac
     9001 caccggccgc accaccggcc cgcaccttca ttacgaagtg cgcatcgatg gcgtggcggt
     9061 gaacccggtg ccctatatgc agggcgaggg cagcggccag ctgccgtcgg gcgcggatgc
     9121 ggtttcggcc gtgcaggccg gccctgccga ggctgacggc gacgattgat cgcgccggcc
     9181 cacaggtttt tcgctttcag gaggcggggg atgcaccatc ccccgccttt ttcgtgcccc
     9241 cgcctttttc gtggccgcct ggcgccggca tatgaaaaag gcggccctgt ggggggccgc
     9301 ctttcattga aggattggcg agaagatcag aggcccagca acttcttggc caccgcgcgc
     9361 acatcatcgg ccatgtcggg ccgttccagc gcgagggcca gcgtcgcttc gacaaagccc
     9421 gtcttcgagc cgcagtcgta gcgcttgccg gcaaaggtga cggcatggaa cggctgctcg
     9481 ccgatcatcc gcgccatggc atcggtcagc tggatttcgc cgccggcgcc cttgccctgg
     9541 gtgtgcagca catccatcac ttcgggctgc aggatatagc ggccggaaat gatgaggttg
     9601 gagggcgctg cctccacctt gggcttttcc acaaggccgg tcacttcggt cagcgcgccg
     9661 ccgaacccgt cgagcttggc gcccggcgcg atcacgccat aggaggacac ttcctcgcgc
     9721 ggcacttcga gcacgctgat gaaattgccg cctagctcgt tataggcttc caccatctgc
     9781 ttcatgcagc ccggcttgcc gatcatcagc tcgtcgggca ggaagatggc gaagggttca
     9841 tcgccgatga tggcgcgcgc gcaccagatg gcatggccca ggcccatcgg cacctgctgg
     9901 cgcacggtga ccagattgcc cggctggatg cgggtgggct cgaggatgtc gaggctcttg
     9961 ccgcgctcgc gcatcgttga ttcgagttcg aaggcggtat cgaaatgttc gacaatcgcc
    10021 gtcttgccgc ggccggtcac aaagatcagc tgctcgattc cggcctctcg ggcttcatcc
    10081 accgcatatt ggatcagcgg ccgatcaata atcggcaaca tttccttggg gatcgccttt
    10141 gtggcgggaa gaaacctggt gccaagcccc gcaacgggga acacggcctt gcgtacgggt
    10201 ttgatcggct tggtattttg catgggttcc tctattgcac cccgacgttt catatgggtt
    10261 aaaacctaag cgtgcgtcga aggcaagttt ggccttcgcc atatttgcgt ggggccgcaa
    10321 gtccgctaag gggccataat ggacaagatc attattcgtg gtggccagaa gctggctggt
    10381 tccgttcccg tttccggcgc caagaatgcg gcgctcacgc tgctgccctg cgcgctgctg
    10441 accgaagagc cattgaccct gcgcaacctg ccgcgcctgg ccgatgtcga cggcttccag
    10501 catctgatga accagttcgg cgtgtccacc gcgattgccg gcgcccggcc cgaggatttc
    10561 ggccgggtga tgaccctgca ggccacccgc ctcacttctt ccaccgcgcc ttacgatctg
    10621 gtgcgcaaga tgcgcgcctc gattctcgtg ctgggcccgc tgctggcccg cgccggcgag
    10681 gcgaccgtgt cgctgcccgg tggctgcgct atcggcaacc gcccgatcga cctgcatctg
    10741 aaggcgctgg aagcctttgg cgcccgcatc gagctggccg ccggctatgt ccgcgcgatc
    10801 gcacccgatg gcggcctgcc gggcggcacc tatgccttcc cggtggtgtc ggtgggggcc
    10861 acggaaaatg cgctgatggc cgctgtgctg gcgcgcggca cctgccggct gaccaatgcc
    10921 gcgcgcgagc cggaaatcgt tgacctgtgc aacctgctgc tggcgatggg cgccgagatc
    10981 gacggcatcg gcacgtcgga tctggtgatt cgcggggtcg accggctgca tggcgccact
    11041 tatcaggtga tgccggatcg catcgaggcg ggctcctatg cttgcgcggc ggcgatcacc
    11101 ggcggcgagg tggagctgcg cggcgcccgg cccgagcaga tggaagccac catccatgcc
    11161 ctgcgcgagg cgggcgtgca tgtggaagtg cgtccgcatg ggttgtttgt ggcggccgat
    11221 ggcccgctgc ggccggtcac gctgtccacc gcgccctatc ccggcttcgc gaccgacatg
    11281 caggcccagc tgatggccct gctgacccgg gccgagggca ccagcgtgct gaccgagacg
    11341 atcttcgaga accgctacat gcatgtgccc gagctgaacc gcatgggcgc caacatcacc
    11401 acccagggcc gcacggcgat cgtgcgcggg gtgccgcggc tgacgggcgc cgaagtgatg
    11461 gcaacggatc tgcgcgcctc gatgagcctg gtgatcgcgg ggcttgccgc cgagggcgag
    11521 acgcaggtcc accgcctcta ccacctcgac cgcggctatg agcggctgga agagaaattc
    11581 gccctgctgg gcgccgagat cgagcgcgtc ggcggggagt gagccggagc ggaccgggcg
    11641 cggggacgga ccgcccccgg cacgccccgg ccctgtcagc cgcgggtata gtagtggcag
    11701 cgcttcttgg cggtcatcgc gcggtcaacg ccgcggccgg caaaggcgcc gcccaccgcg
    11761 cccgcggcaa tgcccaccgg cccgcccagc agcgcatggc ccaccagcgc gccggtggcg
    11821 ccgccggcga tcagcccggt ggtgccgctg gatttgcggc attccttgta gacccgcttg
    11881 gcatgatgcg ggcgcgcctc ggccgatgaa atgcccaccg gcgacatggc gacaacggca
    11941 gtggcgccca gcgccagaat cagcttacgc atgtttcagc ctcctctttt tgcgtggcgc
    12001 ctatgaagcc tgccgggctc taacccagca tgaacgaaag cggggggccg cgttcagcct
    12061 ggcggcgttg gcagggcggc ggccgcagcg ggccgggcgc gggcggccag ccacaggcgg
    12121 atggcgccgg ccaggcccgg cggctggggc gcggcaatgc gctctgcatc gatgcgggcc
    12181 accagcgcgt tgatcggcag gccctcggcc tcggccgcgg cgcgcagcca gtcccagaac
    12241 aatggctcga ggctgaccga ggtgcggtgg ccggcgatct ccaccgagcg tttgacgggc
    12301 gggtgatagg gggtggccat ggccggatgc tagcaggccc gggcagggcg cgaaaagggg
    12361 ggcggcacaa gcaaaacggg gagaaaggct gccctttccc cccgcctgta tctgttcggc
    12421 cgcggccggg atcaatacat gtgctggccg ccgttgatgc tcatcgtcga gccggtgacg
    12481 aagccggcat cttccgagca gaggaaggag accgcgcggg cgatttcctc ggcatggccc
    12541 agccggccca ccgggatctt cgcgacgatc ttttccagca ccggggcggg cacggcggcc
    12601 accatctcgg tgtcgatata gcccggcgca atggcgttca ccgtcacgcc atatttcgcg
    12661 ccttcctggg ccagcgcctt ggtgaagccg tgaatgcccg atttggccgc ggcatagttc
    12721 acctggccat attgccccgc ctgcccgttg atcgagccga tgttgacgat gcggccccag
    12781 ccgcgttcgc gcatgccggg gaaggtggcc ttggccatgt tgaagcagcc gcccagattg
    12841 atgcggatca cctcgttcca gtcgtcgaag gtcatcttct gcagcacgcc atcgcgggtg
    12901 atgccggcat tgttgacgac gatatccacc gggccgaaat cagccgccac ctgggcgatg
    12961 ccatccagcg tggcctgatg gtcgcccacg tcccagcggt aggcggggat gccggtggca
    13021 tcggtgaacg ctttggcctt ttcgacattg ccgccataat tggcaatcac ggtgacgcca
    13081 tcagccttca gcgccaggct gatcgcctcg ccaatgccgc gtgtgccccc ggttacgatc
    13141 gcgatgcgtg tcacaaaacc cctccatact cgaacggtgg tgaggcaagg ctaggtcagt
    13201 cttctggcgg cttcaagaca acttccatcc aattagaaat atttgatttt gccgcgccgc
    13261 aacattaatt cgcaagtgca gcatggaaat gcgcatcttg cgaatgtcac gcggtttcag
    13321 ccctgcgcca tgcccttgtc gggggtattc agaagtggaa ctgcgtgccc acgaacaccg
    13381 cctggctgtc ggccttgccg tcagacagcg gcttcagccg gtcgcggtcc tgcgagtagc
    13441 gcacgccggc ggtgacatcg aagttgcggg tgatggcata gctgccgccc acgcccacgc
    13501 tctgatcgcc attgggatcg agcgggtggg ggctgcggcc ggtgtggtcc ttgtcgtcca
    13561 gcgtgattcg cggggtgagg cgcgggcttg cctggccgcg cgtgccatcg ctgatgtaga
    13621 agccggagat gtcgggcgtg tcggcgcggt ggctgtcggc gcccagcgcc agcccgttgc
    13681 cggccgcggc aaagccctgg ttgccccgcg cgatgcccag attataagcc gccggcgcaa
    13741 tccgcagcac cgccgcattc atgcccgggc gcggctgggc gggcccgcgc acgatgatcg
    13801 tgcgggcggt ggcatcatcc acccgcactg ccacggtgac cgagcgatcc ggccgggtgg
    13861 acaggcccgc cggcgtgaag cggaaggccg ggccgggccc tgtggggctg ctggcgatgg
    13921 tgcgggccag atgcggatcg acccggccgg gggcaaaact gcgcaggcct tcattggcgc
    13981 tgctttcgcc gggggcctgt tccagccggc tggaaaaggc cagaacggcg ctgggcaggg
    14041 ccagcaggca caccgccgcg cccatcacca gcgcgggctg gaagccgccc tgctgcaccg
    14101 gcacgctgcg gcctgcgcct gccgggcgcg gctctgccgg tgcctgcggt gctgccctgt
    14161 tcaacatgcc catgccttaa gccgacccac gccctaaacc gaaaatcccc cgcgcggcct
    14221 ggctgtgccg gccgcccggg tgcgcgcaca ataggccttt tcctgccccg cgccaagccg
    14281 gcgctgcggc agctcgacgc gtatcgcccc ttgccgcgcc gtttgcggca gctatagagg
    14341 cttctgcgag tgtctgtgcg agggccgtcg cgggcaggca gggcgttcgc gccttcgttg
    14401 tgcccggggg cggtgcggcg gcaggcacag ggattttgta acaacgggat tagccaagac
    14461 gatgactgct gcccaagctt ccggccatcc gattttccgc gcgtcgcttc ggggcggcgt
    14521 gctttgcgcg gcgctgctgg ccctggccgg gtgcagcaac gggcatctgg ccttcaagaa
    14581 ccccatccac atcaagcatc accgcgaaaa ccgccacaag cccgatctgc cgcccagcga
    14641 tgtgacgacg atcggcgtca attccttcct gtggcgcgcc agcctcgatg tgctgagctt
    14701 catgccgctg gtgaaggccg acagcaatgg cggcgtgatc gtgaccgact ggtatgtgaa
    14761 cccggccaac cctgccgagc gcatgaagat caccgtgacg atcctcgaca aggatctgcg
    14821 ttcggatgcg ctgcgggtgg cggccagccg gcaggtgcag caggccggcg gctgggtgga
    14881 ggcgccggtg caggcggcca ctgtccagaa gctggaggat gtgatcctga ccaaggcgcg
    14941 cgatctgcgc cgcgacacga tcatccagta agcgcccggc cctccgcggg gggcaggcag
    15001 atatttggcc gatggcccgc cttgcgccag cgcggggcgg gctgtggctt taaaaggggc
    15061 ggccggcgct gcccggcaag cgccggcaag caggccggac cagacaggac agatggcaag
    15121 atgagtgagc gtttcgatcc ggcaaccgca gatgcccgct ggcaatccgt gtgggacgag
    15181 gcgcaaagct tccgcgccga cgatgcgagc accaagccgc gttcctatgt gctggaaatg
    15241 tttccctatc cttccgggcg catccatatc ggccatgtgc gcaactacac gatgggcgat
    15301 gtgctggccc gcttcaagcg gatgcagggc tttgaagtgc tgcacccgat gggctgggac
    15361 gcgtttggca tgccggccga aaacgccgcc atggaaaagg gcgtgcaccc cggcggctgg
    15421 acgcgtgaca acatcgccaa catgaaggcg cagctcaagc gcctgggctt cgcgctggac
    15481 tggagccgcg agatcgccac ctgcgagccg gactattacg gccaggaaca ggcgctgttc
    15541 ctcgaccttt atgccgccgg cctggtctat cgcaaggaaa gcgcggtcaa ttgggatccg
    15601 atcgactgca cggtgctggc caacgagcag gtgatcgacg ggcgcggctg gcgctctggc
    15661 gcgctggtgg aaaagcgcaa gctcaaccag tggttcctga agatcaccga ttttgctgac
    15721 gagctgctcg atggcctctc cacgctggag cactggcccg agaaggtgaa gctgatgcag
    15781 gaaaactgga tcggcaagag ccagggcctg caatttgcct tccagctgtc gaacggcgcc
    15841 agcctcgagg tgtataccac ccggcccgac acgatctttg gcgccagctt tgtggcggtg
    15901 gccgccgatc atccggtggc gcaagcgatc ggcaccgatg aagcgcgcga tttcattgcc
    15961 ctgtgcaagc agggcggcac caccgcggca gagctggaaa ccgccgaaaa gctgggcttt
    16021 gacaccggcc ttaccgcccg gcaccccttc accggggcgg atctgccggt ctatatcgcc
    16081 aatttcgtgc tgatggatta tggcaccggc gcgatcatgg cggtgccggg ccatgaccag
    16141 cgcgatttcg atttcgccac caaatatgcc ctgccgatca cccgcgtggt ggccgccagc
    16201 gccgaggatg ctgccaggcc cttcggcacc gaggcggagg ctggcgatgg cgtgctggtc
    16261 aattccggtt tcctggacgg cctgcccgtg gctgaggcca agcgggcggt gattgcccgc
    16321 gccgaggcgg aaggctgggg cgagggcaag acggtgtggc gcctgcgcga ctggggcgtt
    16381 tcgcgccagc gctattgggg cacgccgatt ccctttgtgc attgcgaagt gtgcggcgtg
    16441 gtgccggtgc ccaagaagca gctgccggtc accctgcccg aggatgtcga tttctcggtg
    16501 ccgggcaatc cgctcgatcg ccatcccacg tggaagcatg tcgattgccc gcaatgcggc
    16561 catgccgccc ggcgcgagac cgacacgctc gacacttttg tcgattccag ctggtatttc
    16621 ctgcgctttg ccagccagcc ggccgatcgc cccttcgatc cggccgagat cgcccgctgg
    16681 ctgccggtgg ggcagtatat cggcgggatc gagcatgcga tcctccacct gctctatgcc
    16741 cgcttctgga cccgcgcgct ggcccgcatc ggcaagatcg aggtggcaga gccgtttgcc
    16801 agcctgttca cccagggcat ggtaacgcat gagacctaca gccgcgtgga tgccggcaat
    16861 ggccagccga tctggtatgc gccgggcgaa gtggcgcgtg atggcaatgg cgccacgctg
    16921 atcgccgatg gtgcgccggt ggagatcggc cgcgtcatca agatgtcgaa gtcgaagaag
    16981 aacgtggtgg atccggatga gatcgtggcc aaatatggcg ccgatgcgat ccgctggttc
    17041 atgctgtccg acagcccgcc cgagcgcgac ctgccgtgga gcgaggcggg catcgagggc
    17101 tgcggcaagt tcgtgcagcg gctgtggcgc ctgttcggcc agtatgacgg ggctgccacc
    17161 ggcgaggaca aggcggtgga ccgcaaggcc caccagacca tcgccgcggt tgccggcgat
    17221 atcgaatcgc tgggcttcaa caaggccgtg gcgcgcattt acgagctgac cggcgtggtg
    17281 gaaaaggccg cccccagcgc cagccgcagc gcggccatca aggtggtgct gctgctgatc
    17341 gcgccgatga tgccgcatct ggccgaggaa gcctttgccg ccatgggcgg ggcagggctg
    17401 gtggctgatg ccgcctggcc ggcggtggat ccggcgctgc tggtggatga tgaggtgacc
    17461 attgccatcc agctgaacgg caagctgcgc gacacgctgg tggcccccaa gggcgccgcg
    17521 aaggacaggc tggaagcgat ggcgctggcc aatgccaagg tggtggccgc gctggatggg
    17581 gcaacgccga aaaaggtcat tgtcgtgccc gaccggctag tgaacatcgt cgtatgaagc
    17641 gaatcgccgg ccttgccgct cttgcccttg cgggcgccag cctttccgcc tgcgggctgc
    17701 atccgctgta tgaagggggc ggccatgccg ccgctgccag cggccttgcc ggggtgcaga
    17761 tcacgcccat ccagggccgc gccggctggc tgatgagcgc cgctctggcc gatcgcgtgc
    17821 aggccagggg cagcgcgcgc tatcggctgg atgtgaagct ggaggacaag ctcgaaggcc
    17881 tcggcattct ctccaacgat accgtcagcc gcgagcggcg caccttgcgc gcgcgctatc
    17941 agctggtgga tctggccacc ggcgccatcc tcctcgacca gacggcgggt tctgacgagg
    18001 gtatcgaccc gacttcctcg gaatatgcca ccgtggccgg cgagcagacg gcgctggaaa
    18061 acctcacccg cgtggtggcc gaccggatcg tggcgaacct ggccgtggcg ctgcgggcaa
    18121 gggccgcgca tccgccggcc caggggcagt gaaggccagc cagcgcgaat ttgccggcgc
    18181 cgccccccgt gcggcgcagg gctgcggcgt gttcttcctg tgcgggccgg atgaggccag
    18241 cgtgcaggat gcggcgggcc tgatcgcctc gctgctgccc gatgccggcg agcgagtgga
    18301 gatgaccggc gccgagctgc gccgcgaccc ggtgcggctg ggcgatgagg cgcgttcctc
    18361 ctcgctgttt ggcggcagcc ggcatatctg ggtgcgctct tccggcgagg aagcgcatga
    18421 tgccatcgcc aacctcatcg ccagcgatgt gccctttgcc ccggtggtgg tgcaggcgcc
    18481 cggcgccacc gacaagggca agaccgccaa gctgctggaa cgccgcgagg atgcgctggt
    18541 ggcgatgttc tatccgccgg atttacgttc ggtaacggat gcggtgcggc ggatggccga
    18601 tggcgcgggc ctgcgcatga ccagcgagct ggccgagcag atcgcccggg cggcggggct
    18661 cgacacgcgg ctggcgcagg gcgaggtgac caagctggcg ctctatctcg atgccgatcc
    18721 gcagcggccg cgcaccgccg atgccgcagc ctttgccgaa gtgggcgcgc gcaccgagga
    18781 agaggggatg atgcagctgg tcaacgcggc gctgggcggc gatgggccgg cgctggcggg
    18841 cgaattgcgc cgcatggccg agctggggct gaacggcgtg gcggtgctgc tggcgtgcga
    18901 gcggcgggcc gcgcaactgg cgcagatggc cgcgcggctg gggccgggcg gcgatgtgcg
    18961 cgcctttgtg gcggcagagc ggcaggcgcg gcggatcttc tggaaggatg aggccgatat
    19021 tgcccgccag ctgcgcatct ggcgcggccg cgcgctggaa cggctggcgg agcgtctgct
    19081 tggccttcac cgcgccctgc tggccaacag ccagagcgcc gacc
//
LOCUS       DASRYE010000089        11153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_199937,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000089 DASRYE010000000
VERSION     DASRYE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199937"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFF98_05130"
     CDS             <1..1116
                     /locus_tag="VFF98_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZU63045.1"
                     /translation="RAALAAANAAADAAHLRARRLEQEAASAGAEADRAAHEAAAIAA
                     RVQQAEADITAAEAHAAVIAGQSRALEADLARRQQPLIQLTAALQRLSRRPLVLSLLR
                     PGTLADTVHSRALIAATLPRISAETAGIRRQLAALRALRGQAQEAAAALRRHQGELAA
                     ERGRLGTLEGARRLAARDAHAGADREAEQADALAAHARNLGDLVAGLNAAAALSDRLA
                     RLPGPVLRPAAGGASALPAQSAGPEVAAAPQRFALPVTGRLIAGFGGASQGGASQGLS
                     IAARAGAEAAAPAAGKVGFAGPYPGYGQIVIIDHAGGWTSLVTGLAAVDVRAGDAVLA
                     GAPLGVAGPGHPVVGYELHRAGGAVNPLDYLAAGLPG"
     gene            1223..2563
                     /locus_tag="VFF98_05135"
     CDS             1223..2563
                     /locus_tag="VFF98_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZU63046.1"
                     /translation="MGVKTAALLRAGLLLGAVAALPVTTAGLAAVDGRIAPQFGRLFT
                     VYQLVKANYVDPTDDDKLVKGAIDGMLASLDPHSSYLEGASLERLDTMIDGSYAGLGL
                     SVVMDDSAVKVVSPMRGSPAEKAGVKAGDYITHLDGKLIYGGDLDEAVAQMRGPAGTQ
                     IRLTIYRPGRDQPFDVTVTRGVIELRPVTSSVDGTIGIITVNEFSRDVGKEAWQAIQD
                     MRKQTGGRLSGLVLDLRSNPGGALDEAVALGDLFITKGDIVSQRGRRPSENIVYRAET
                     VFPGDAAAGLPLIVLIDAGSASASEIVAGALQDDHRALVMGERSFGKGSVQTMIRLDK
                     DHAVKLTTARYYTPSGRSVQEGGIQPDVRVPQITDPDARKRNEDAVRESDLRGHLVNE
                     AGVDDKKLEADKTPDPRFKMSAEELKTKGITDFQLWYALQTLRHAANGVVVPRR"
     gene            2583..3068
                     /locus_tag="VFF98_05140"
     CDS             2583..3068
                     /locus_tag="VFF98_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZU63047.1"
                     /translation="MMASSQGKAHLVALAVPVGALGGALAFQYFGGLYPCEMCWWQRY
                     AHMAALLLAVVAIVLPGARRLFIGLAGLALVAAAVVSAYHAGVEYGWWKGLTACTSDI
                     DFAAGGDPLKAILDSPVVRCDVVQWKLFGISLAGYDFLISGLAALAVFALLARSTKEQ
                     N"
     gene            3068..3595
                     /locus_tag="VFF98_05145"
     CDS             3068..3595
                     /locus_tag="VFF98_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HZU63048.1"
                     /translation="MANPLSRKLDRILRVDQAGEYGAVRIYAGQMAVMGSRGPHAGKI
                     AHMQEQEVDHREQFDRLLAARGGRPTALQPIWHVAGFALGAATALISPEAAMACTAAI
                     ETEIDAHYTRQLEELEPGEDDELEALITRARDEEREHRDTALASGAEQAPAYPLLSGA
                     IRLGCKVAIALSERI"
     gene            3744..4367
                     /locus_tag="VFF98_05150"
     CDS             3744..4367
                     /locus_tag="VFF98_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63049.1"
                     /translation="MRQFAPRRHRPAAALAGALFALAGAFAALPAHAQGTNADGGSDK
                     VNMVIIYGDDKCPESHGDQITVCARKAESERYRIPEPFRNTSGPDNEAWTNRVIAYER
                     VGAAGTQSCSPVGAGGWTGCSGQFIKNAYAEKKAETDVNFQKMISDARAKRMEGVDAE
                     AVETQKRVEAAEKAYQERERARQDAAEATGKKANDGLSEPLPGAQGK"
     gene            complement(4467..5270)
                     /gene="fabI"
                     /locus_tag="VFF98_05155"
     CDS             complement(4467..5270)
                     /gene="fabI"
                     /locus_tag="VFF98_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZU63050.1"
                     /translation="MTGLMQGKRGLIMGLANDKSLAWGIAQQLHAQGAEMAFSFQGEA
                     LEKRVRPLAESLGSDFLIDCDVSDMEALDAAFAALKERWGTIDFIVHAIGFSDKRELR
                     GRYYDTSLDNFLLTMNISAYSLVAVTKRAIELMPNGGSILTLTYYGAEKVMPHYNVMG
                     VAKAALETSVKYLANDCGPANIRVNAISAGPIKTLAASGIGDFRYILKWNELNAPLRR
                     NVTIEDVGGSGLYFLSDLSSGVTGEIHHVDGGYNVVGMKQEDAPDIALG"
     gene            complement(5267..6202)
                     /locus_tag="VFF98_05160"
     CDS             complement(5267..6202)
                     /locus_tag="VFF98_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZU63051.1"
                     /translation="MRGGGPAGSHARAGRHWAVARRVVASVWRDGFIHAGNLAYMAIL
                     ALFPFFVVISALFTLVGEGRQRAASVDAFLAALPPVVAQVLDPVGRAVIDARHGWLLW
                     AGGLISLWTVSSLIETLRDILRRAYGITPARAFWLYRLISTGMIMGAVVMLLVSLYAQ
                     VVMSTAEELVRSRLDGLGWLWGHVSLSRMATTLALFGSIYVLFLGLTPGAYRRRRYPK
                     WPGALLVTLWWALVSEGLPVLLRLSFKYDLTYGSLAGVMITLFFFWLVGLGMVTGAEL
                     NAALAATPERRSSLGRTPDADEDAGPPNEDKVVDE"
     gene            complement(6213..7178)
                     /locus_tag="VFF98_05165"
     CDS             complement(6213..7178)
                     /locus_tag="VFF98_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZU63052.1"
                     /translation="MAQDPYATLGVARGASEKDIKSAYRKLAKELHPDHNQDNPKAAE
                     RFSEVTRAYDLLSDKDKRARFDRGEIDIDGNPAFAGGAGFGGGGFGGGQRGFRPEDFG
                     GGGAGVDIGDIFEGLFGGRGPGAGMGGGLGGGGGFGGRGRQAPRGADVRYRLAVSLPD
                     AAIAASQRLTLSDGKTIDLKLPAGLEDGIQMRLAGKGEPGPGGAGDALVTIQIQPHPF
                     FTRDGDDIRLDLPVGLGEAVHGAKVKVPTVEGAVMLSVAAGSSSGRVLRLKGRGFTRK
                     DGTRGDQLVKLEIALPAAGSAAAQDLAQRLEGWTDSADIRARLGS"
     gene            7290..7922
                     /gene="pdxH"
                     /locus_tag="VFF98_05170"
     CDS             7290..7922
                     /gene="pdxH"
                     /locus_tag="VFF98_05170"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZU63053.1"
                     /translation="MTDEREAIREAVTGTDPMALFDAWFAEARAKEPNDPEAVALATA
                     TPGAAPSVRMVLMKDHGPHLGDGAGFVFYTNSHSRKGGELLGNPQAAMLFHWKSLRRQ
                     IRIEGSIAPVPDEMADAYFASRSRDSQIGAHASQQSQPLASRARFIADIAGVVTRYPL
                     GKVPRPPHWTGFCLKAEAIEFWMDRPFRLHERRRYTRDGAGGWASTLLYP"
     gene            7938..8864
                     /locus_tag="VFF98_05175"
     CDS             7938..8864
                     /locus_tag="VFF98_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZU63054.1"
                     /translation="MSGSHDSHALSRSAAFASIATASLLLGMKGWAAWTTGSTAMLGS
                     LADTGLDLVASIATLLGVWVAGQAPDENHRFGHGKAEAISAMFQVVLIALSAAALAVH
                     AVQQLFTGERTTDAGDGILASGVAIAATLALLAWQRHVLARTRSIAIATDHMHYQADL
                     MLNGAVIVALLLDQYAGFTRADPLFGIGIALWLAYGAWEAGQEAIEQLMDHEWHDDKK
                     QRFIEVLARHPELRGVHDLRTRTSGNRDFVQFHVWVDGSMSVTEAHRVMDEIEDKLHA
                     EFPDVEILIHPDPEGHVDERGVAAEDILPHTA"
     gene            complement(8898..10031)
                     /locus_tag="VFF98_05180"
     CDS             complement(8898..10031)
                     /locus_tag="VFF98_05180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZU63055.1"
                     /translation="MVPRLPFGFRLIAPLALLPALIGCHGAQPAQGPLSAEALGAVVA
                     HPGPSRQALARAVDALFTEKGLGETRALLVVSKGQLVAERYAPGFGKDTRFIGWSMSK
                     TVTGLLIGMLVSDGQLRLDMGAPIPAWQRPGDPRGEITLRQLLQMRSGLAHVENSDPP
                     QLADTARMLFLDGRDNMAAYAESQPLVAEPGKRWVYSTATSVILADIATRALTDSADA
                     ALRRRVLDEYFHTRLTQPLGLKSMVAEYDAAGTMLGGSMIYATARDWGRLGEFLRQGG
                     RASGTQVVPVDWIRFMTEPSPANPGYGGQLWINRPQSTGKEELFPGRAPASLFALIGH
                     LGQYVLVSPDQQLVVVRLGKSTEEERVAVRDRLADIVELFPAR"
     gene            10061..10456
                     /locus_tag="VFF98_05185"
     CDS             10061..10456
                     /locus_tag="VFF98_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63056.1"
                     /translation="MATIKLKRQVWAGGGLAGLLAALRRRVPWGMRFGLGLAVAGLLL
                     AGGWLLPGLLAKVPLATAYSARVACACRYVAGRDLASCRQDLEAGTELVRLTEDAASH
                     TITARMLLFWAAHATYTPGPGCVLEPWKR"
     gene            complement(10476..>11153)
                     /locus_tag="VFF98_05190"
     CDS             complement(10476..>11153)
                     /locus_tag="VFF98_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HZU63057.1"
                     /translation="GAELHRAVDPARDQSYFLYATTEAQLDYLRFPLGGMEKPQVRAL
                     AQHFGLRVAAKPDSQDICFVPDGDYASVVRRVRPEAGMGGAIVHAETGAVLGEHKGII
                     HYTVGQRRGLEIGGQAEPLYVVGIDAAQARVLVGPRRLLAVGAARITETNRIGPLDAA
                     EPLCVKVRSLARPVPAVLAGPLGDGAETLIRFAGPEYGVAPGQAAVLYAGDRVVGGGW
                     IAETFAA"
BASE COUNT         1778 a         3825 c         3845 g         1705 t
ORIGIN      
        1 cgcgccgccc ttgccgccgc caatgccgcg gccgatgccg cccatctgcg cgcccgccgg
       61 ctggagcagg aggccgcttc ggccggcgcc gaggccgacc gtgccgcgca tgaagccgcc
      121 gccatcgccg cccgcgtgca gcaggccgag gccgacatca ccgccgccga ggcgcacgcg
      181 gccgtcatcg ccgggcagag ccgggcgctg gaggcggatc tcgcccgccg ccagcagccg
      241 ctcatccagc tgaccgccgc gttgcagcgc ctgtcgcgcc ggccgctggt gctgtccctg
      301 ctgcgcccgg gcacgctggc ggatacggtg cacagccgcg cgctgatcgc cgctaccttg
      361 ccgcgcattt ccgccgaaac ggccggcatc cgccgccagc tggcggccct gcgcgcgctg
      421 cgtggccagg cgcaggaggc cgccgcggcc ctgcgccgcc atcagggcga gctggcggca
      481 gagcgcggcc gcttgggcac gctggaaggg gcccgccgcc tggccgcccg cgatgcccat
      541 gccggcgccg accgcgaggc cgagcaggcc gatgcgctgg ctgcccatgc ccgcaatctg
      601 ggcgatctgg tggccggcct caatgccgcc gctgccctgt cagaccggct ggcgcggctg
      661 ccggggccgg tgctgcgccc ggcggcgggc ggcgcgtctg ccctgccggc gcagagcgcc
      721 gggcctgagg tggccgccgc gccccagcgc tttgccctgc cggtaacggg ccggctgatc
      781 gccggttttg gcggggcctc gcaagggggg gcctcgcagg ggctcagcat cgccgcgcgg
      841 gcaggggccg aggcggccgc gcccgctgcc ggcaaggtgg gttttgccgg gccctatccg
      901 ggctatggcc agatcgtcat catcgatcat gccggcggct ggacaagcct ggtgacaggc
      961 cttgccgcgg tggatgtgcg ggcgggcgat gcggtgctgg caggcgcccc gctgggcgtt
     1021 gccgggcccg gccatccggt tgtcggctac gagctgcacc gggcgggcgg ggcggttaac
     1081 ccgctggatt atctggccgc aggattgccc ggatgagggc cattgtgctg gccaaggggc
     1141 accgcaaggt gcaaggcaag cctagcgctt gccggccgca ggcgctaggc ttgcggccag
     1201 gtttgagcag gaaaggctga gaatgggtgt gaagactgcc gcgctgctgc gtgccggctt
     1261 gctgctgggg gccgtggctg ccctgcctgt caccacggcg gggctggcag cggtggatgg
     1321 ccgcatagcg ccgcaattcg ggcggttgtt caccgtctat cagctggtca aggccaatta
     1381 tgtcgatccc acggatgacg acaagctggt gaagggggcg atcgatggca tgctggccag
     1441 cctcgatccg cattcctcct atctcgaagg cgccagcctc gagcggctcg acacgatgat
     1501 cgacggttcc tacgcagggc ttggcctcag cgtggtgatg gacgacagcg cggtgaaggt
     1561 ggtctcgccc atgcgcggca gcccggcgga aaaggccggc gtgaaagcgg gcgattacat
     1621 cacgcaccta gatggcaagc tgatctatgg cggcgacctt gacgaggcag tggcgcagat
     1681 gcgcggcccc gccggcaccc agatccgcct caccatctat cgcccggggc gcgaccagcc
     1741 cttcgacgtg acagtgacgc gcggggtgat cgaactgcgc ccggtcacct cctcggtcga
     1801 tggcacgatc ggcatcatca ccgtcaacga attcagccgc gatgtcggca aggaggcctg
     1861 gcaggccatc caggacatgc gcaagcagac cggcgggcgc ctgtcgggcc tggtgctgga
     1921 tctgcgctcc aacccgggcg gcgcgctgga tgaggcggtg gcgctgggcg atctgttcat
     1981 caccaagggc gatatcgtct cgcagcgcgg ccgccgcccg tcggaaaaca tcgtctaccg
     2041 cgccgagacg gtgttccccg gcgatgccgc cgcgggcctg ccgctgatcg tcttgatcga
     2101 tgccggctct gccagcgcca gcgagatcgt tgccggcgcc ctccaggatg atcaccgcgc
     2161 cctggtcatg ggcgagcgca gcttcggcaa gggcagcgtg cagacgatga tccggctgga
     2221 caaggaccat gccgtgaagc tgaccactgc gcgctactac acgccttcgg gccgtagcgt
     2281 gcaggaaggc ggcatccagc ccgatgtgcg cgtgccgcag atcaccgatc ccgatgcgcg
     2341 caagcgcaac gaggatgccg tgcgcgaaag cgacctgcgc ggccatctcg tcaacgaggc
     2401 cggggtggac gacaagaagc tggaagccga caagaccccc gacccccgct tcaagatgag
     2461 cgccgaggaa ctgaagacca agggcatcac cgatttccag ctgtggtatg ccttgcaaac
     2521 cttgcggcat gcggcaaatg gcgtggtggt gccgcgccgc tgaggcgctg cttgaggaga
     2581 cgatgatggc ttccagtcag ggcaaggcgc atctggtggc gctggcggtg ccggtgggcg
     2641 cgctgggcgg cgcgctggcc ttccagtatt tcggcgggct gtatccttgc gagatgtgct
     2701 ggtggcagcg ctatgcccat atggcggcgc tgttgctggc ggtggtggcg attgtgctgc
     2761 cgggcgcgcg gcggctgttc attggcctgg ccggcctggc gctggtggca gcggcggtgg
     2821 tttcggccta tcatgccggg gtggaatatg gctggtggaa ggggcttacc gcctgcacca
     2881 gcgatatcga ttttgccgcg ggcggcgatc cgctgaaggc cattctcgat tcgcccgtgg
     2941 tgcgctgcga tgtggtgcag tggaagctgt tcggcatttc gctggccggc tatgatttcc
     3001 tcatctcggg gctggcggcg ctggcggtgt tcgccctgct ggcccgctcg accaaggagc
     3061 agaactgatg gccaaccctc tctcgcgcaa gctcgatcgc attctccggg tcgatcaggc
     3121 gggcgaatat ggcgcggtgc gcatctatgc cgggcagatg gcggtgatgg gatcgcgcgg
     3181 gccccatgcc ggcaagatcg cccatatgca ggagcaggag gtggatcacc gcgagcagtt
     3241 cgaccggctg ctggccgcgc gcggcggccg gcccacggcg ctgcagccga tctggcatgt
     3301 ggccggcttt gcgctggggg cggcaacggc gctgatcagc ccggaagccg ccatggcctg
     3361 caccgctgcc atcgagaccg agatcgacgc ccactacacc cgccagctgg aagagctgga
     3421 gccgggcgag gacgacgagc tggaagcgct gatcacccgc gcccgcgacg aggagcgcga
     3481 gcaccgcgac accgcccttg ccagcggcgc cgagcaggcc ccggcctatc cgctgttgtc
     3541 gggcgcgatc cggctgggct gcaaggtggc gatcgccctt tccgaacgca tctgatctgc
     3601 gcctttttgc cctgtgcctt tttgcgctga cgcaagcgcg cctgcttgcg cttcagctat
     3661 cattcacggc cccgctctcc tatagcgggc cacaggggcg ccggcccttc cacacacgcc
     3721 tttcgcagcc caaggaacgt ttcatgcgcc agttcgcccc gcgccgccat cgccctgccg
     3781 cagcccttgc cggcgcgctt ttcgcccttg ccggcgcctt tgcggcgctg cccgcccatg
     3841 cccagggcac caatgccgat ggcggttccg acaaggtgaa catggtcatc atctatggcg
     3901 atgacaaatg cccggaatcg catggcgacc agatcaccgt ctgcgcccgc aaggccgaga
     3961 gcgagcgcta tcgcatcccc gagcccttcc gcaacacctc cgggcccgac aacgaagcct
     4021 ggaccaaccg cgtgatcgcc tatgagcgcg tgggcgctgc cggcacgcag agctgctcgc
     4081 cggtgggcgc gggcgggtgg acgggctgtt cgggccagtt catcaagaac gcctatgccg
     4141 agaagaaggc cgaaaccgac gtcaacttcc agaagatgat ctcggatgcg cgggccaagc
     4201 gcatggaagg cgtggatgcc gaggcggtcg agacgcaaaa gcgcgtggaa gcggccgaaa
     4261 aggcctatca ggagcgcgag cgcgcccgcc aggatgccgc cgaggccacc ggcaagaagg
     4321 ccaatgacgg gctgagcgag ccgctgccgg gcgcccaggg caagtaaccg gcgccaaggc
     4381 acgcaaaaag ggcggcgggg cacacacccc gccgcccttt ttgctgcagc gccgttaggc
     4441 cagggttcga tcaggccaag gcccgattaa cccagggcaa tatccggcgc gtcttcctgc
     4501 ttcatgccca ccacattgta gccgccatcc acatggtgga tctcgccggt cacgcccgag
     4561 gacaggtccg agaggaaata gaggcccgag ccgccgacat cctcgatcgt cacattgcgg
     4621 cgcagcgggg cgttcagctc gttccacttg aggatatagc ggaaatcgcc gatgccgctg
     4681 gcggccaggg tcttgatcgg gccggccgag atggcgttga cgcggatgtt ggcgggcccg
     4741 caatcattgg ccagatattt cacgctggtt tccagcgccg ccttggccac gcccatcacg
     4801 ttgtaatgcg gcatcacctt ctcggcgcca taataggtga gggtgaggat cgagccgcca
     4861 ttgggcatca gttcgatggc gcgcttggtc acggccacca ggctgtaggc cgagatgttc
     4921 atcgtcagca ggaaattgtc gaggctggtg tcgtagtagc ggcccctcag ctcgcgcttg
     4981 tcggaaaagc cgatggcgtg gacgatgaaa tcgatcgtgc cccagcgttc cttcagcgcg
     5041 gcaaaggccg cgtccagcgc ttccatgtcc gacacatcgc aatcgatgag aaaatcgctg
     5101 cccaggcttt cggccagcgg gcgcacgcgc ttttccagcg cctcgccctg gaaggagaag
     5161 gccatttcgg cgccctgtgc gtgcaactgc tgggcaatgc cccaggccag cgacttgtcg
     5221 ttggcaaggc ccatgatcag gcctcgttta ccctgcatca atccggtcat tcgtctacaa
     5281 ccttgtcctc atttggtggg ccggcgtctt cgtcggcgtc tggcgtgcgg cccaggcttg
     5341 atcgtcgctc gggcgtggcg gccagcgcgg cgttcagttc ggctcccgtt accatcccta
     5401 agcccaccag ccagaaaaag aaaagcgtga tcatcacgcc ggccaggctg ccgtaggtga
     5461 gatcgtattt gaagctgagg cgcagcagca ccggcagccc ctcgctgacc agcgcccacc
     5521 acagcgtgac cagcagcgcg cccggccatt tgggatagcg ccgccgccga taggcgcccg
     5581 gcgtaagccc cagaaacagc acgtagatag agccgaacag ggccaaggtg gtggccatgc
     5641 gcgagaggct gacatggccc cacagccagc ccagcccatc cagccggctg cgcaccagtt
     5701 cctcggcggt gctcatcacc acctgcgcgt agagcgagac cagcagcatc accaccgcgc
     5761 ccatgatcat gccggtggag atcagccgat agagccagaa ggcccgcgcc ggggtgattc
     5821 cataagcgcg gcgcagaatg tcgcgcaggg tttcgatcag gctggagacc gtccacaggc
     5881 tgatcaggcc gcccgcccac agcagccagc catggcgcgc atcgatcacc gcgcggccca
     5941 ccggatcgag cacctgcgcc accaccggcg gcagcgcggc cagaaaggca tccaccgagg
     6001 ccgcccgctg ccgcccttcg cccaccagcg tgaacagcgc gctgatcacc acgaagaagg
     6061 ggaacagcgc caatattgcc atataggcga ggttgccggc gtggatgaag ccatcgcgcc
     6121 acacgctggc caccacccgg cgggccacgg cccagtgccg ccccgcccgg gcatggctgc
     6181 cggccggccc cccgccgcgc atcgatctgg cctcagctgc ccagccgggc gcggatatcg
     6241 gcgctgtcgg tccagccttc cagccgctgg gcgaggtctt gcgccgccgc gctgccggcg
     6301 gccggcaggg caatctccag cttcaccagc tggtcgccgc gggtgccatc cttgcgggtg
     6361 aagccgcggc ccttgaggcg cagcacccgg ccagaggaac tgcccgcggc aacgctcagc
     6421 atcaccgcgc cttccactgt gggcaccttc accttggcgc catgcaccgc ctcgcccagg
     6481 cccaccggca gatcgaggcg gatatcatcg ccatcgcggg tgaagaaggg gtgcggctgg
     6541 atctggatcg tcaccagcgc atcgcccgcc ccgcccgggc ccggctcgcc cttgccggca
     6601 aggcgcatct ggatgccatc ctcaaggcct gctggcagct tcaggtcgat cgtcttgcca
     6661 tcagacaggg tgaggcgctg gctggcggcg attgccgcat cgggcagcga gaccgccagg
     6721 cgatagcgca catcggcccc gcgcggcgcc tgccggccgc ggccgccaaa gccgccgccg
     6781 ccacccaggc ccccgcccat tccggccccg ggcccgcgcc cgccaaacag gccctcgaag
     6841 atatcgccga tatcgacacc cgcgccgccg ccgccaaaat cctccggccg aaagccgcgc
     6901 tggccgccgc cgaaaccgcc accgccaaag cccgccccgc cggcaaaggc ggggttgccg
     6961 tcgatgtcga tctcgccgcg gtcgaagcgg gcgcgcttgt ccttgtccga gaggaggtca
     7021 taggcgcgcg tcacttccga gaagcgctcg gcggccttgg ggttgtcctg gttgtggtcg
     7081 gggtgcagtt ccttggccag cttgcgatag gcggacttga tgtccttctc gctggcgccg
     7141 cgcgccacgc ccagggttgc ataaggatct tgtgccatgg cgcatagcta ggtgcccggg
     7201 cgggcgcctg caagagccag atcgtactgg ccatccagag cattctcttc taacgcgggc
     7261 ggcgaacgca atgccttggg gaagcgcaga tgactgacga gcgcgaagcc atccgggagg
     7321 cggtgaccgg caccgatccg atggccctgt tcgatgcctg gttcgccgag gcgcgcgcaa
     7381 aagaacccaa cgatcctgaa gccgtggcgc tggccaccgc cacgccgggc gcggcccctt
     7441 cggtgcgcat ggtgctgatg aaggaccacg ggccccatct gggcgatggc gccggctttg
     7501 tgttctacac caacagccac agccgcaagg gcggggaact gctgggcaac ccgcaggcgg
     7561 ccatgctgtt ccactggaag agcctgcgcc ggcagatccg catcgagggc agcatcgccc
     7621 ccgtgcccga cgagatggcc gatgcctatt tcgccagccg cagccgcgat tcgcagatcg
     7681 gcgcccatgc cagccagcaa tcgcagccgc tggccagccg ggcgcgcttc attgccgaca
     7741 tcgccggtgt cgtcacccgc tatccgctgg gcaaggtgcc ccgcccgccg cactggacag
     7801 gcttctgcct gaaggccgag gcgatcgaat tctggatgga ccgccccttt cgcctgcacg
     7861 agcgccggcg gtatacgcgc gatggcgccg gcggctgggc cagcaccctg ctctatccct
     7921 gaaaggggcc tgatccgatg agtggttcac acgattcgca tgcgctcagc cgcagcgccg
     7981 cctttgccag catcgccacg gccagcctgc tgctggggat gaagggctgg gcggcgtgga
     8041 cgacaggctc caccgccatg ctgggcagcc tggccgatac cgggctcgat ctggtcgcct
     8101 cgatcgccac cttgctgggc gtgtgggtgg cagggcaggc gccggatgag aatcaccgct
     8161 tcggccatgg caaggccgag gccatctcgg cgatgttcca ggtggtgctg atcgccctgt
     8221 cggccgcggc gctggcggtg catgcggtgc agcagctgtt caccggcgag cgcaccacag
     8281 atgccggcga tggcatactg gcctctggcg tggcgattgc ggccacgctg gcgctgctgg
     8341 cctggcagcg ccatgtgctg gcgcgcaccc gctccatcgc cattgccacc gaccatatgc
     8401 attatcaggc cgatctgatg ctgaacgggg cggtgatcgt ggcgctgttg ctcgatcaat
     8461 acgccggctt cacccgggcc gatccgctgt ttggcatcgg cattgcgctg tggctggcct
     8521 atggcgcctg ggaagccggg caggaggcca tcgagcagct gatggaccat gagtggcacg
     8581 acgacaagaa gcagcgcttc atcgaggtgc tggcccgcca cccggaattg cgcggcgtgc
     8641 atgatctgcg cacgcgcacc agcggcaacc gcgatttcgt gcagttccac gtgtgggtgg
     8701 atggcagcat gagcgtgacc gaggcccacc gcgtgatgga cgagatcgag gacaagctgc
     8761 acgccgaatt tcccgatgtg gaaatcctca tccatcccga tccggaaggg catgtggatg
     8821 aaaggggcgt ggcggccgag gatatcctgc cgcacacggc ctgatttgca ggcggcgccg
     8881 gcccgcgccc cttggtctca acgcgccggg aacagctcga cgatatcggc cagccggtcg
     8941 cgcacggcca cgcgctcttc ctcggtggat ttgcccagcc gaacgaccac cagctgctga
     9001 tcgggcgaga ccagcacata ttgccccaga tggccgatca gcgcgaacag gctggccggc
     9061 gcgcggccgg gaaacagctc ttccttgccg gtgctttgtg ggcggttgat ccacaattgc
     9121 ccgccatagc cggggttggc ggggctgggc tcggtcatga agcggatcca gtccaccggc
     9181 accacctgcg taccgctggc cctgccgccc tggcgcagga actcgcccag ccggccccag
     9241 tcacgcgcgg tggcatagat catcgagccg cccagcatgg tgccggccgc atcatattcg
     9301 gccaccatcg atttcagccc cagcggctgg gtgaggcggg tgtggaaata ttcgtcgagc
     9361 acgcgccggc gcagggcggc atcggcactg tcggtcagcg cgcgggtggc gatatcagcc
     9421 aggatcacgc tggtggcggt ggaatagacc cagcgcttgc ccggctctgc caccagcggc
     9481 tggctttcgg cataggcggc catattgtcg cgcccgtcga ggaagagcat gcgcgcggta
     9541 tcggccagct gcggcggatc gctgttttcc acatgggcca ggcccgatcg catctgcaac
     9601 agctggcgca gggtgatctc gccgcgcgga tcgcccgggc gctgccaggc gggaatcggc
     9661 gcgcccatgt cgagccgcag ctgcccgtcg gaaaccagca tgccgatgag aaggcccgtc
     9721 accgtcttcg acatcgacca gccgatgaag cgggtgtcct tgccgaagcc gggggcatag
     9781 cgttcggcca ccagctggcc tttggagacg accagcaggg cgcgcgtttc gcccagcccc
     9841 ttttcggtga acagcgcatc caccgcccgc gccagcgcct ggcgcgaagg gccgggatgg
     9901 gccaccaccg cccccagcgc ctctgccgaa aggggcccct gcgccggctg ggcaccatgg
     9961 cagccgatca gggctggcag cagcgccagg ggcgcgataa ggcggaagcc gaaaggcagg
    10021 cggggcacca tgggcgtgtc catgcgcaag cgggcaggca atggcaacca taaagctgaa
    10081 gcgacaggtg tgggcaggcg gcgggctggc ggggctgttg gcggcactgc gccggcgggt
    10141 gccctggggg atgcgattcg ggctggggct ggccgtggcg gggctgttgc tggcgggcgg
    10201 gtggctgctg ccgggcctct tggccaaggt gccgctggcc acggcctata gcgcccgggt
    10261 ggcctgcgcc tgccgctatg tggcggggcg ggatctggcc agctgccggc aggatctgga
    10321 agcgggcacc gaactggtgc gcctgacaga ggatgccgcc agccacacga tcaccgcgcg
    10381 catgctgctg ttctgggcgg cccacgccac ctacacgccc ggccccggct gcgtgctgga
    10441 gccgtggaag cgctgatcgg agtcagccgg gcgaatcagg cggcgaaggt ctcggcgatc
    10501 cagccgccgc ccaccaccct gtcgccggca taaagcactg cggcctggcc gggcgccacg
    10561 ccatattcgg ggccagcaaa gcggatcagc gtttcggcgc catcgcccag cggccctgcc
    10621 agcactgccg gcaccggccg ggccagcgaa cgcaccttca cacacagcgg ctctgcggca
    10681 tccagcgggc cgatgcggtt cgtctcggtg atgcgcgcgg cgcccaccgc cagcagccgg
    10741 cgcggcccca ccagcacgcg cgcctgcgcc gcatcgatgc ccaccacata cagcggctct
    10801 gcctgcccgc cgatctccag cccccggcgc tggcccacgg tgtagtggat gatgcccttg
    10861 tgctcgccca gcaccgcgcc cgtttcggca tggacaatgg cgccgcccat gcccgcctct
    10921 ggccgcaccc gccgcacgac gctggcataa tcgccatcgg gcacgaagca gatatcctgc
    10981 gaatcgggct tggcggccac cctgaggcca aaatgctggg ccagcgcgcg cacctgcggc
    11041 ttttccatgc cgcccagcgg aaagcgcaga taatcgagct gggcctcggt ggtggcataa
    11101 aggaaatagg attgatcgcg cgccggatcc acggcgcggt gcagctcggc gcc
//
LOCUS       DASRYE010000090         4768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_252246,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000090 DASRYE010000000
VERSION     DASRYE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252246"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1161)
                     /gene="rpoH"
                     /locus_tag="VFF98_05195"
     CDS             complement(250..1161)
                     /gene="rpoH"
                     /locus_tag="VFF98_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZU63058.1"
                     /translation="MTSDSNLPATPAAPSTEAGLNRYLAEIKKFPVLTPEQEYMLAKR
                     YAEHQDPEAARQLVTSHLRLVAKIAMGYRGYGLPMSELIAEGNIGLMQGVKKFEADRG
                     FRLATYAMWWIKASIQEFILRSWSLVKIGTTAAQKKLFFNLRRMKKNIDAYEDSDLHP
                     DDVKHIATHLGVPEAEVVAMNRRMMMGGDASLNVSLNEEGEGQWQDMLADAGPLQDET
                     VADAEEARWRHAVLTEAMASLNERERAILIQRRLVDEPQTLEELSQTYNVSRERVRQI
                     EVRAFEKLQKALLKLATDKRLLAGAGA"
     gene            complement(1205..2245)
                     /locus_tag="VFF98_05200"
     CDS             complement(1205..2245)
                     /locus_tag="VFF98_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZU63059.1"
                     /translation="MNQGETLLQGVIGTPGRLDRALAEASALSRERVKTLLAEGRVEV
                     GGRPAGNGSAKVAQGLAFAIRLPAPAPLAAEAQDIPLVIAHEDEHLIVVDKPAGLVVH
                     PAAGNPDGTLVNALLHHCAGRLSGIGGVARPGIVHRIDKDTSGLLVVAKSDAAHEGLA
                     RQFADHSIERAYLCLTAGVPMPPQGTLRGIIARSDRDRKKMALLPEGARLPAEGGRGK
                     HAITHYRLREALAGAALVECRLETGRTHQVRVHMASIGHALLGDPVYGSASKSLRTLL
                     AQLGFARQALHATVLGFIHPVSGETLRFASDLPADMRELLARLGSLHADQPGFFLPEP
                     PSGAWPPPRDAA"
     gene            2262..2660
                     /locus_tag="VFF98_05205"
     CDS             2262..2660
                     /locus_tag="VFF98_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZU63060.1"
                     /translation="MDVTLARGIIATLLAEAARAHPQEACGLLLGPAQGPIRAVVPCA
                     NVAADPARHFEIDPAALIAAHRAARGGGPAVLGYYHSHPEGPPVPSATDRAMACGDGR
                     IWAIVGEGRLRCWRDGPLGFEALSTREIDG"
     gene            2726..3397
                     /locus_tag="VFF98_05210"
     CDS             2726..3397
                     /locus_tag="VFF98_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HZU63061.1"
                     /translation="MTSDMIDRTEALDLASLLCSRMCHDLLSPVGAMTNGLELLADEQ
                     DPDMRRRCFELLEQSAASTAAKLKFFRLAFGAAGGFGDSVAVAEARALIEGLIAGREK
                     LELNWQITVDALPKPAVKTVLNLAMIAIEALVRGGRVDVAAELRQDLEVPATEIVVRA
                     IGPRIAFDPVVGQALEGKLAPEELSSRTAPARMLANIAARLGGVLQFAQVPEALVLGA
                     VLPLA"
     gene            3413..4153
                     /locus_tag="VFF98_05215"
     CDS             3413..4153
                     /locus_tag="VFF98_05215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZU63062.1"
                     /translation="MDELITREVPSPNWNARQLPISMVVLHYTGMRSAAEAIARLTDP
                     AAEVSAHYLIDEDGTVLRMVAEEHRAWHAGRSYWRGITDVNSASIGIELVNPGHEFGY
                     RPFTDAQMEALIPLLAGIIARHNIEPANVVGHSDVAPARKADPGELFEWETLARYGLA
                     MATPAPRIRLLHENPGAFFLSLERYGYDIADTEAAVTAFQRRFRPRIIDGQIDGEVGA
                     LLFELLLARDTDARDSLDPDCDDSDFPF"
     gene            4217..4664
                     /gene="rnpB"
                     /locus_tag="VFF98_05220"
     ncRNA           4217..4664
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFF98_05220"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          736 a         1575 c         1631 g          826 t
ORIGIN      
        1 cgccggcgct ggccgcggcc accagccggt cgccccattc ggccatgtcg gcttccacgg
       61 cctgggccag cgtggcggca atttccgcgc ccagcgcctc gaattgttcg cgggcaagat
      121 cgcggtcgat cgtctggatc atcggtgggg tggctttctg tggggggaag gggcagccgg
      181 caccgcttcg ccaggcgaag ggcgccggcc cctgtgtgcc ggcaaatccc ccaacaaaag
      241 gttaccgctt caggcgccgg cgccggccag caggcgcttg tcggtcgcca gcttcagcag
      301 cgccttctgc agcttttcaa aagcgcgcac ctcgatctgg cgcacccgct cgcggctgac
      361 gttataggtc tgcgaaagtt cctccagcgt ctgcggctcg tccaccagcc ggcgctggat
      421 gaggatggcc cgctcgcgct cgttgaggct ggccatcgcc tcggtcagca ccgcatggcg
      481 ccagcgcgct tcctcggcat cggccacggt ctcgtcctgc agcgggccgg catcggccag
      541 catgtcctgc cactggccct cgccttcctc gttgaggctg acattgaggc tggcatcgcc
      601 gcccatcatc atgcgccggt tcatcgccac cacttcggcc tcgggcacgc cgagatgggt
      661 ggcgatgtgc ttcacatcgt cggggtggag gtcgctgtcc tcataggcgt cgatgttctt
      721 cttcatccgc cgcaggttga agaacagctt cttctgcgcc gccgtggtgc cgatcttcac
      781 caggctccag ctgcgcagga tgaattcctg gatgctcgcc ttgatccacc acatggcata
      841 ggtggccagg cgaaagcccc tgtcggcctc gaatttcttc acgccctgca tcaggccgat
      901 attgccctcg gcgatcagct cgctcatcgg caggccatag ccgcgatagc ccatggcgat
      961 cttggccacc agcctgaggt ggctggtcac aagctggcgc gcggcctcgg gatcctgatg
     1021 ctcggcatag cgcttggcga gcatgtattc ctgctcgggc gtgagcaccg ggaacttctt
     1081 gatttccgcc agatagcggt tcagcccggc ctctgtggag ggggctgctg gcgtcgccgg
     1141 caggttgctg tcgctggtca tcggcatggt cctcgtcaaa tgtcagctca cccttgaaag
     1201 acccttaagc ggcatccctc ggcggtggcc acgcgccgga gggcggttcc ggcagaaaaa
     1261 aaccgggctg gtcggcatgt aaactcccca gccgggcaag cagttcccgc atatcggcgg
     1321 gaagatcgct ggcgaaccgc agagtctcgc cagaaacggg gtggataaac cccagcacgg
     1381 tggcatgcaa tgcctggcgg gcaaagccca gctgcgccag cagggttcta agcgatttgg
     1441 atgcgctgcc atatacggga tcacccaata gcgcatggcc gatggacgcc atgtgaacgc
     1501 gcacctgatg ggtgcgcccg gtttccagcc ggcattccac cagcgcggct ccggccagcg
     1561 cctcgcgcag gcggtaatgg gtgatggcat gcttgccgcg gccgccttct gccggcaggc
     1621 gcgcgccttc gggcagcagg gccatcttct tgcggtccct gtccgatcgg gcgatgatgc
     1681 ccctgagggt gccctggggc ggcatgggca cgcccgctgt caggcacaga taggcccgct
     1741 cgatcgaatg gtcggcaaac tggcgggcca ggccttcgtg cgcggcatcg ctcttggcca
     1801 ccaccagcag gcccgatgtg tccttgtcga tccggtggac gatgcccggc cgcgccaccc
     1861 cgccaatgcc ggaaaggcgt ccggcgcaat ggtgcagcag cgcgttcacc agcgtgccgt
     1921 cggggttgcc ggcggccggg tgcaccacaa gccccgcggg cttgtccacc acgatcaggt
     1981 gctcgtcctc atgggcgatg acgaggggaa tatcctgcgc ctcggcggcc agcggggcag
     2041 gggcgggcag gcggatggca aaggccagcc cctgcgccac cttggcagag ccattgccgg
     2101 ccggccggcc gcccacttcc acccggccct cggccagcag cgtcttcacc cgctcgcgcg
     2161 acagcgcgct cgcctcggcc aaagcgcggt cgagacggcc gggcgtgccg atgacgcctt
     2221 gcaacagtgt ttccccctgg ttcattaccc tccacatggg catcgacgtg acgcttgcaa
     2281 ggggcattat cgccacactt ctggcagaag cggcgcgcgc gcacccgcaa gaggcctgcg
     2341 gattgctgct ggggccggca caggggccga ttcgcgcggt ggtgccttgc gccaatgtcg
     2401 cggccgatcc ggcccgccat ttcgagatcg accccgcagc gctgatcgcc gcccaccgcg
     2461 cggcaagggg tggcgggccg gcggtgctgg gctattacca ttcgcacccc gaagggccgc
     2521 cggtgccttc tgccaccgat cgcgccatgg cctgcggcga cgggcggatc tgggcgattg
     2581 tgggcgaggg gcggctgcgt tgctggcgcg atggcccctt aggcttcgag gcgctttcca
     2641 cgcgcgagat tgacggctag gacagcacgc ctcccggcca tatctttctg caattttgta
     2701 tgcgccgaag tgtgaaagac ctgcgatgac gagcgatatg attgaccgca ccgaggccct
     2761 tgatctggcc agcctgctgt gttcgcgcat gtgccatgat ctgctctcgc cggtgggggc
     2821 gatgaccaac gggctggaac tgctggccga cgagcaggat ccggacatgc gtcgccgctg
     2881 ctttgaactg ctggagcaga gcgcggcttc caccgccgcc aagctcaagt tctttcgcct
     2941 ggcctttggc gcggccggcg gctttggcga cagcgtggcc gtggccgagg cccgcgcgct
     3001 gatcgagggg ctgatcgccg ggcgcgagaa gctggagctg aactggcaga tcaccgtgga
     3061 tgcgctgccc aagccggcgg tgaaaaccgt gctcaacctg gcgatgatcg ccatcgaggc
     3121 gctggtgcgc ggcgggcgcg tggatgtggc ggccgaactg cgccaggatc ttgaggtgcc
     3181 ggcgaccgag atcgtcgtgc gcgcgattgg cccgcgcatt gccttcgatc cggtggtggg
     3241 ccaggcgctg gaaggtaagc tggcgcccga ggagttgtcg agccgcacgg cgcctgcgcg
     3301 gatgctggcc aatatcgctg cccggctggg cggggttctg caatttgcgc aagtgcccga
     3361 ggcgttggtg ctgggggcgg tgttgccgct ggcctgagcg gaaggaggcg gcatggacga
     3421 gctgatcacc cgcgaagtgc catcgcccaa ctggaacgcg cgccagttgc cgatcagcat
     3481 ggtggtgctg cattacaccg gcatgcgcag cgcggcagag gcgatcgccc gcctcaccga
     3541 tccggccgcc gaagtctccg cccattatct catcgacgag gatggcacgg tgctgcgcat
     3601 ggtggccgag gaacaccgcg cctggcatgc cgggcgcagc tattggcgcg gcatcaccga
     3661 tgtcaattcg gccagcatcg gcatagagct ggtgaacccg ggccatgaat tcggctatcg
     3721 ccccttcacc gatgcgcaga tggaagcgct gatcccgctg ctggccggca ttatcgcccg
     3781 ccacaatatc gagccggcca atgtggtggg ccattccgat gtggcgccgg cgcgcaaggc
     3841 ggatccgggc gaattgttcg aatgggaaac gctggcccgc tatggcctgg cgatggccac
     3901 gcccgctccg cgcatccgcc tgctgcacga gaaccccggc gccttcttcc tcagccttga
     3961 gcgctatggc tatgacattg ccgatacaga agcggccgtc actgccttcc agcgccgctt
     4021 tcgcccgcgc attatcgacg ggcagatcga tggcgaggtg ggcgcgctgc tgttcgaact
     4081 gctgctggcg cgcgacaccg atgcgcgcga ttcgctcgac cctgattgcg acgacagcga
     4141 ttttcccttc tgagcggggg cccttcttgc atcccgcttg ccgcgctggc gccccggtgc
     4201 taacgcgcgc cccacaccag ggggccgggc agccgcgtca tcgcttgcga tgccgaggaa
     4261 agtccgggct ccacgaaatg agggtggcgg gtaacgcccg cccggtgggg cgcctgccgc
     4321 aaggcagggg ccgaagccga gggacagtgc cacagagagc agaccgccaa ggggccgcaa
     4381 ggccctgcgg caagggtgaa agggtgcggt aagagcgcac cgcggggctg gtaacagcac
     4441 cggcatggta aaccccaccc ggagcaagac cgaatagggg cggggcgcct tcgcaaggag
     4501 gccggcctgt ttcgagccga ggccgcaagg cctgtcgccg cccgggttgg ttgctggagc
     4561 gtgcggagca atccgccgcc tagaggaatg gctgccgctg gctgccgcgt ccgcaaggat
     4621 acgagcagcc ttcacagaac ccggcttaca ggccccctgg tgttttcggt tgtgttttcg
     4681 gttcaagctg agcttgcggc tccggccctc tcccccaccc ggccactctt agggtactat
     4741 cgttgggtgg ccgggtaggg gagagggc
//
LOCUS       DASRYE010000091         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_21398,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000091 DASRYE010000000
VERSION     DASRYE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21398"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1099
                     /locus_tag="VFF98_05225"
     CDS             305..1099
                     /locus_tag="VFF98_05225"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU63063.1"
                     /translation="MKEEIASARLGLFVCGAQKGGTSALAAYLRQHPGLSPPPAKELH
                     FFDDEARCWARPDYTALEAGFAPADGRLRYECTPAYGFWRPALARIAAYHPAARLVFV
                     LRDPFHRAWSHWCMEYARGAESLPFAEAIRAGRRRLRGRGGLDPAWRAWSYVERGFYG
                     LQLARARGHFPAAQILCLRSEDLAADPAGTLARLAGFAGLAPFPPLAPLRVHEAPRRA
                     WPCAPTEADRALVMAELAADLADCTRLSGLDTGGWGTCLPAVRSPA"
     gene            complement(1083..2090)
                     /locus_tag="VFF98_05230"
     CDS             complement(1083..2090)
                     /locus_tag="VFF98_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZU63064.1"
                     /translation="MQPGTCRRWPLAAAFAPLALVAGAPPLAAQTESAPAQSALAAGQ
                     DTVSINALTSSPQPASTDPQAATLAGPPAPAIHWLAEASGGIADRDGGPRGGFAVTGL
                     SRKLGDYYLRGAFTAYRSTLERSDTALPSTYLVGTLGGGGRFGHWVFDGWASYGRQQY
                     GEINGATGPRSSPFHSSPYWALGADFGRILRLGPHWYATPTLTLTYTYSRLLHPGYHN
                     DGTDDFQSSEPALTGIAAIRLDHTLGRAQQSLIGLGYSHHATSNGLSTVTLLGFDTQS
                     GHAVPELGVLHRRDGWEELAGNATLRLTHRLWLDLSVVRSFGAAAGDTTTGSAGFRLG
                     F"
     gene            complement(2094..>3070)
                     /locus_tag="VFF98_05235"
     CDS             complement(2094..>3070)
                     /locus_tag="VFF98_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU63065.1"
                     /translation="AVAPAPEPFPNSWQLRVMAWFACSLLIVSPLAWLFARRIVRPLQ
                     GFARAAEALGRDPSTTIMPLSGPAEIGRAAYAFNQMQNRLRAFVDDRTAMVGAISHDL
                     RTPLTRLRFRIEDAPDGLRDGLLKEVTEMEQMISQVITFIRDASTPGPRETLDLADLV
                     AGTVRDARLIGGRVELEPSAHAPVEVDPVGMRRLLGNLLENAVKYGEHARVRVAVRGD
                     EAVAEVIDDGPGIPMDELDHVFEPFYRTEAARKSGKPGTGLGLAVCRSIARAHGGDVA
                     LARRADGFVAQVRVPLVFDRRKGLDVPPRAEPVAPAPAPAAPIVRRAA"
BASE COUNT          438 a         1033 c         1139 g          460 t
ORIGIN      
        1 cgacgggaca tgggcaagtt cctcgattct tctgcgttgg ttgcacgggt cgcccgatgg
       61 cggcgtttgc tccctaaagg caggagcggc cccaaaaggc aagccccggc aaacgaggag
      121 cagcttgggc aagctggctc gcgcggccgg ggcgtggcag gccatatagg cgatctgcgt
      181 ggattcttca actggcgctg aaaagggggg gatggatccg tttcggcggg gccgggcggg
      241 caatggctcc atcctgcggg gcatcgcttg gcggcgggcg ggggcggggc taaccccttg
      301 acggatgaag gaggagattg ccagcgcccg cctgggcctg ttcgtctgcg gagcgcagaa
      361 agggggcacc agcgcgctgg ctgcctatct gcgccagcat cccggcctct cccccccgcc
      421 cgccaaggag ctgcattttt tcgacgatga ggcgcgctgc tgggcccgcc cggattacac
      481 cgcgctggaa gccggctttg ccccggcaga tggccggctg cgctatgaat gcacgccggc
      541 ctatggcttc tggcggccgg cgctggcgcg cattgcggcc tatcatccgg ccgcgcggct
      601 ggtgtttgtg ctgcgcgatc cgtttcacag ggcctggtcg cactggtgca tggaatatgc
      661 ccggggcgca gaaagcctgc cctttgccga ggcgatccgc gctggccggc ggcggctgcg
      721 cgggcggggc gggctggatc cggcgtggcg ggcgtggtcc tatgtggagc gggggttcta
      781 tgggctgcag ctggcgcggg cgcgggggca tttcccggcg gcgcagatcc tgtgcctgcg
      841 atcggaagat ctggcggcgg atccggccgg cacgcttgcg cgcctggccg gtttcgccgg
      901 gctggcgccc tttccgcccc ttgcgccctt gcgcgtgcat gaagcgccgc gcagggcctg
      961 gccgtgcgct cccacagagg cggaccgggc gctggtgatg gccgagctgg ccgcggacct
     1021 ggccgattgc acgcggcttt cggggctgga taccggcggc tggggcacct gcctgcccgc
     1081 cgtcagaagc ccagcctgaa gccggcgctg ccggtggtgg tgtcgcccgc ggccgcgcca
     1141 aagctgcgca ccacgctcag atccagccac aggcgatgcg tgaggcgcaa ggtggcgttg
     1201 ccggccagct cctcccaccc gtcgcgccgg tgcagcacgc ccagttccgg cacggcatgg
     1261 ccgctttgcg tgtcaaaccc cagcagcgtc acggtggaca ggccattgct ggtggcatgg
     1321 tgcgaatagc caaggccgat caggctttgc tgcgcgcggc ccagcgtatg gtccagccgg
     1381 atggcggcaa tgccggtcag tgccggctcg gacgactgga aatcatccgt gccatcattg
     1441 tgatagcccg ggtggagcag gcggctgtag gtgtaggtga gcgttagcgt gggggtggca
     1501 taccagtgcg gccccagccg caggatgcgc ccgaaatccg cccccagcgc ccaatagggg
     1561 ctgctgtgga aagggctgct gcgcgggccg gtggcgccat tgatctcgcc atattgctgc
     1621 cggccatagc tggcccagcc atcgaacacc caatggccaa agcgcccgcc gccgcccagc
     1681 gtgcccacca gataggtgga gggcagggcg gtgtcgctgc gttccagcgt gctgcgatag
     1741 gcggtgaagg cgccgcgcag atagtaatcg cccagcttgc ggctgagccc tgtcacggca
     1801 aagccgccgc gcgggccgcc atcgcgatcg gcgatgccgc cgctggcttc ggccagccag
     1861 tggatggcgg gcgccggcgg gccggcaagg gtggcggcct gcgggtcggt gctggctggc
     1921 tgggggctgc tggtcagcgc gttgatcgac accgtgtcct gcccggcggc aagggcggat
     1981 tgggcgggcg cgctttccgt ttgcgcggcc agtggcggcg cgccggcaac aagggccaac
     2041 ggggcgaaag cggcggccag cggccaccgg cgacaggtgc cgggctgcat ggatcaggcc
     2101 gcgcgccgca cgattggcgc ggccggcgcg ggggccgggg ccacaggctc ggcgcgggga
     2161 ggcacatcca gccccttgcg ccggtcgaac accagcggca cgcgcacttg tgccacaaag
     2221 ccatctgctc gccgcgccag cgccacatcg ccgccatggg cccgcgcgat ggagcggcac
     2281 accgccagcc ccagcccggt gcccggcttg ccggatttgc gcgccgcctc ggtgcgatag
     2341 aagggctcga acacatggtc gagctcgtcc atcgggatgc ccgggccatc gtcgatcacc
     2401 tcggccaccg cctcatcgcc gcgcacggcc acccgcacgc gcgcatgctc gccatatttc
     2461 accgcgtttt cgagcagatt gcccagcagg cggcgcatcc ccaccggatc cacctcgaca
     2521 ggggcgtggg cgcttggctc cagctccacc cggccgccga tcagccgcgc atcgcgcact
     2581 gtgccggcca ccagatcggc caggtccagc gtctcgcgcg ggccgggggt ggaagcatcg
     2641 cggatgaagg tgatgacctg gctgatcatc tgctccatct cggtcacttc cttcagcagc
     2701 ccatcgcgca ggccatcggg ggcatcctcg atgcggaagc gcagccgcgt gaggggggtg
     2761 cgcagatcgt ggctgatggc ccccaccatc gccgtccggt cgtcgacaaa ggcgcgcagg
     2821 cggttctgca tctggttgaa ggcataggcc gcgcggccaa tctccgccgg gccggaaagc
     2881 ggcatgatgg tggtggacgg atcgcgcccc agcgcttcgg cggcgcgggc aaagccctgc
     2941 agcgggcgca cgatgcgccg cgcaaacagc caggccagcg ggctgacgat cagcagcgag
     3001 caggcaaacc aggccatcac cctgagctgc cagctgttgg ggaacggctc tggcgcgggg
     3061 gccacggcca
//
LOCUS       DASRYE010000092         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_235169,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000092 DASRYE010000000
VERSION     DASRYE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235169"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFF98_05240"
     CDS             <1..609
                     /locus_tag="VFF98_05240"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZU63066.1"
                     /translation="PMHKIKANVDKQLAACGEAPFYTLGPLTTDVAPGYDHITSAIGA
                     AMIGWFGTAMLCYVTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAKLRDDAL
                     SRARFDFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKSAHFCSMCGPKFCSMKITQEVR
                     DFAAKQNAPIETFAAAEEAEQGMAQMAEKYREGGDLYMPAAE"
     gene            676..1077
                     /locus_tag="VFF98_05245"
     CDS             676..1077
                     /locus_tag="VFF98_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63067.1"
                     /translation="MVSTPLPDLAIRLARAAFNRALAAGDVAAIGPLLAPAALLVTGT
                     DSAVISGRKAQILAWKREFAAPGRTTYTRTPETITVSPVEPIAMEHGRWTGTDADGLP
                     LASGTYAAKWRNLAPGGAQWVIEAEIYVTLG"
     gene            complement(1086..1682)
                     /locus_tag="VFF98_05250"
     CDS             complement(1086..1682)
                     /locus_tag="VFF98_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZU63068.1"
                     /translation="MSRISKIDHTDWDPELRAITHADEASVLERGTLRIMAHKPETAK
                     GFVRFFAALKAERSLPERLLELVRLRIAYHNQCRSCMAIRYAGPAAAGVDEALVCTLE
                     RPMEADDLTPAEKLAIDYGERLATNHLSLDDAFHDRLRAHFTEAQIVELGMFCAVCVG
                     FGRLGASWDMVEELPQAFRQRDGGPIAPWQQAEVIPVR"
     gene            complement(1856..2140)
                     /locus_tag="VFF98_05255"
     CDS             complement(1856..2140)
                     /locus_tag="VFF98_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63069.1"
                     /translation="MIKSLFAAAALIAVPAIAQAEEAKSTFTYQGVDYTYTAAQEGNV
                     KVLRGTALNGTAPFELRVTKSGVSGTFDSQPVNFPLREVKHIDAADIAVR"
     gene            complement(2160..2399)
                     /locus_tag="VFF98_05260"
     CDS             complement(2160..2399)
                     /locus_tag="VFF98_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63070.1"
                     /translation="MIRHRGAISRRHIRLRQKNIRHTGLAFTGTSVCFGADLIGKQGI
                     VAHFAMEMPFNAFACNCRSHMLRCNKITGARGNHS"
     gene            complement(2438..3391)
                     /locus_tag="VFF98_05265"
     CDS             complement(2438..3391)
                     /locus_tag="VFF98_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HZU63071.1"
                     /translation="MPAPCRPRIATLTMNPAIDGACEAEAVFPTHKIRTHAERYDPGG
                     GGINVARVLARLGHAVEVAYLAGGATGALLDELLDQHGLERCRIPIHDHTRQSLSVFE
                     RSTGREFRFVPDGPLLAESEWQAAFDHCLALGREGGPHGWLVASGSLPRGVPVDFYAR
                     LAQGLAGSGLRLAVDTSGPALAAAMAAGGLFLAKPSQGEFEALVGRKLENAEAVGRAA
                     LELVRAGKAAHVAVSMGHEGAVLAHAGGVLIHPALDVPVKSATGAGDSFVAGLLHGFL
                     LGEEAAGALRWAMAAGAAAVLNPGTDLCHPEDLQRLHASLA"
     gene            complement(3427..3744)
                     /locus_tag="VFF98_05270"
     CDS             complement(3427..3744)
                     /locus_tag="VFF98_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HZU63072.1"
                     /translation="MSDDDYVYDEESGEWMPAAELAARRAASAQASGGQIEVRDAVGN
                     LLADGDQVTLIKDLDVKGAGQTLKRGTLIKSIRLTGDAQEIDCKFDGIKGLVLRAEFV
                     KKR"
BASE COUNT          637 a         1361 c         1334 g          674 t
ORIGIN      
        1 ccgatgcaca agatcaaggc caacgtcgac aagcagctgg ccgcctgcgg cgaggcgccc
       61 ttctatacgc tggggccgct gaccaccgac gtcgcgccgg gctatgacca catcacgtct
      121 gcgatcggtg cagccatgat cggctggttc ggcacggcga tgctctgcta tgtcaccccc
      181 aaggagcacc tgggcctgcc cgaccgtgac gatgtgaagg tgggcgtggt gacgtacaag
      241 ctggccgccc atgcggcgga cctcgccaag ggccacccgg ccgccaagct gcgcgacgat
      301 gccctcagcc gcgcccgttt cgacttccgc tggcgcgacc agttcaacct cagcctcgat
      361 cccgatacgg ccgagcagta tcacgaccag acgctgccgg ccgaaggggc caaatcggcg
      421 catttctgct ccatgtgcgg ccccaaattc tgctcgatga agatcacgca ggaagtgcgc
      481 gattttgccg cgaagcagaa cgccccgatc gagaccttcg ccgcggccga ggaggccgag
      541 cagggcatgg cgcaaatggc cgagaaatac cgcgagggcg gcgatctcta catgccggcg
      601 gccgaatagt ctgcccttgg cacaggcgcc gcgcggcagg gcttctcttg cccgcggcgc
      661 ctgtgtagag cccggatggt gagcacgccc ctaccagacc ttgccatccg cctggcccgc
      721 gccgccttca accgcgcttt ggctgccggc gatgtggccg ccatcggccc gctgctggcg
      781 cctgccgcgc tgcttgtcac cggcacggac agtgcggtga tcagcggccg caaggcgcag
      841 atccttgcct ggaagcgcga atttgccgcg cccgggcgca ccacctacac ccgcacgccc
      901 gaaaccatca cggtgagccc cgtagagccc atcgccatgg agcacggccg gtggaccggc
      961 accgatgccg acggcctgcc gctggcctct ggcacctatg cggccaaatg gcgcaatctg
     1021 gcgccgggcg gcgcgcaatg ggtgatcgag gcggagatct acgtgacgct ggggtgaggt
     1081 gggcttcagc gcaccgggat cacctcggcc tgctgccagg gggcaatggg gccgccatcg
     1141 cgctggcgga aggcctgggg cagttcctcc accatatccc acgaggcgcc cagccggcca
     1201 aagcccacgc acaccgcgca gaacatcccc agctcgacga tctgcgcctc ggtgaaatgc
     1261 gcgcgcagcc tgtcgtgaaa ggcatcgtca aggctgagat ggttggtggc cagccgctcg
     1321 ccataatcga tcgccagctt ttcggccggg gtcaggtcgt cggcttccat cgggcgttcc
     1381 agcgtgcata ccagcgcctc gtccactccg gcggcggccg ggccggcata gcggatggcc
     1441 atgcagctgc ggcactggtt gtgataggca atgcgcagcc gcaccagttc cagcagccgt
     1501 tcgggcaggc tgcgctcggc cttcagcgcg gcgaagaagc gcacgaagcc cttggccgtc
     1561 tcgggcttgt gggccatgat ccgcaaggtg ccgcgctcca gcacgctggc ttcgtcggca
     1621 tgggtgatgg cgcgcagttc cgggtcccag tcggtatggt cgatcttgct gatgcgagac
     1681 atgtgctctc ccctttttcg ggagaggata gcaggctggc gcctgtgccc cacctgccgg
     1741 aaagcctagc atccatgcag caaaacgccc ccgccggcat gggcaggggc gctttgctga
     1801 ggcctttgct gaggccaagg gagggcggcg gggcaggccc cgcgcggccg ctctcttagc
     1861 gcacggcgat gtcggcagcg tcgatgtgct tcacttcgcg cagcgggaag ttcaccggct
     1921 ggctgtcgaa ggtgcccgac acgccgctct tggtgacgcg cagctcgaag ggggcggtgc
     1981 cgttcagcgc ggtgccgcgc agaaccttga cattgccttc ctgggcggcg gtataggtat
     2041 agtccacacc ctgataggtg aaggtggact tggcttcctc ggcctgcgcg atggcgggca
     2101 cggcgatgag ggcagcggcg gcaaacagcg acttgatcat ggcaatatcc cctgattggt
     2161 taggaatgat tgcctcgggc acccgttatc ttgttgcatc gcagcatatg gcttcggcag
     2221 ttgcaggcaa atgcattaaa gggcatttcc attgcaaaat gcgcaacaat gccctgcttg
     2281 ccgataaggt cggcgccaaa acagacactt gtgccggtga acgccagccc ggtgtggcgg
     2341 atattttttt gccgcaaacg tatgtgccgc cggctgatcg cgccgcgatg gcgaatcata
     2401 tgccatttcg gtttccatat tggccctgga taaccattca ggcgaggctg gcgtgcagcc
     2461 tctgcagatc ttccgggtgg cacaggtctg tgccggggtt gagcacggcg gcagcgcctg
     2521 cggccatcgc ccagcgcagg gcgccagccg cctcttcgcc cagcaggaag ccgtggagca
     2581 ggccggcgac gaaactgtcg cccgcgccgg tggcgctttt caccggcaca tccagcgccg
     2641 ggtggataag cacaccgccc gcatgtgcca gaacggcacc ttcatgcccc atgctgaccg
     2701 ccacatgcgc cgccttgccc gcgcgcacca gttccagcgc ggcgcggccc acggcctcgg
     2761 cattttccag cttgcggccc accagcgcct cgaattcgcc ctggctgggc ttggccagga
     2821 acaggccgcc ggcggccatg gccgcggcca gcgccggccc gctggtatcc actgccagcc
     2881 gcaggccgct gccggccagc ccctgcgcca gccgggcgta gaaatccacc ggcacccccc
     2941 gcggcagcga gccggaggcc accagccagc catgcggccc cccttcgcgg cccagcgcca
     3001 ggcaatggtc gaaggcggcc tgccattcgc tctcggccag cagcgggcca tcgggcacga
     3061 agcggaattc gcgcccggtg ctgcgctcga acacgctgag cgactggcgg gtgtgatcgt
     3121 ggatggggat gcggcagcgt tccagcccgt gctgatccag caattcgtcg agcagggcgc
     3181 ccgtggcccc gcccgccaga taggccactt ccaccgcatg gcccagccgc gccagcaccc
     3241 gcgccacatt gatccccccg ccgcccggat catacctttc ggcatgggtg cggatcttgt
     3301 gggtggggaa caccgcctcg gcctcgcagg cgccatcgat ggcagggttc atcgtcagcg
     3361 tggcgatgcg gggccggcaa ggggcgggca tgcgggggtg ttcctgctgg gctgcggcgg
     3421 gttgcctcag cgcttcttca cgaattcggc gcgcagcacc agccccttga tgccatcgaa
     3481 cttgcagtcg atctcctgcg catcgcccgt cagccggatc gacttgatga gcgtgccgcg
     3541 cttcagcgtc tggccggcgc ccttcacgtc gaggtccttg atcagcgtca cctgatcgcc
     3601 atcggcgagg aggttgccca ccgcatcgcg cacctcgatt tgcccgccgg atgcctgcgc
     3661 actggctgcg cgccgcgccg ccagttcggc cgccggcatc cattcgccgc tctcttcgtc
     3721 atacacgtaa tcgtcgtcgc tcatctgtgc ctcccgtggc gtgtgggccg cccatgccag
     3781 cttgcggggg gcagcgccag tcttctgcgc aaaaggggta ttctgcgcaa aggcgggcgt
     3841 taaccccttt gtcaggctgg gttcacttgg ccattgctac gtaaagggcg aatgcgttgc
     3901 aggaggattt gagatggcac gccggaaggt tgacagggtg gggctggcgt tgggcacggg
     3961 ggcctgcgcg ctgatgttgg cggcaagccc ggtgctgggc gcggcc
//
LOCUS       DASRYE010000093         8692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_146926,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000093 DASRYE010000000
VERSION     DASRYE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146926"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="VFF98_05275"
     CDS             complement(<1..2397)
                     /locus_tag="VFF98_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZU63073.1"
                     /translation="MAVLGPTNTGKTHLAIERLCAHSSGAIGFPLRLLAREVYEKVCA
                     IKGRERVALITGEERIEPPGARWLLCTAEAMPARADLAFMALDEVQLCADRERGHVFT
                     DRLLNMRGREETMLLGSSVMEPMVKALVPEAEIISRPRFSTLSHAGAKKLSRIPPRSV
                     IVAFSAEQVYAVAEMLRRFRGGAAVVMGALSPETRNKQVALYQAGEVDYLVATDAIGM
                     GLNLDVGHVAFAGLSKFDGVRQRRLTPTEMAQIAGRAGRHQRDGTFGTLAGSGGHDAE
                     FGEDEVFAIEEHRFAPLTRLFWREAEPRFDTLARLIADLEAPPLLPELAPAPQAIDLA
                     VLKILAEDPAIARGLQLPGQIRRFWHVCSLPDFRNQGAETHARFVARLWEELSRGTLA
                     SDHAPRAISALDNTAGDIDTLQGRIAAIRSWAYICQRPDWVLNRSEMAERARGVEARL
                     SDALHGRLTERFVNRRTTVLMRRLGPDAALLPVLLEGEDVLVEGEKLGSLRGFRFRVD
                     PVARLADQKLLLAAAEKHLPQLLAVRAQELIAALKQPAGRRGAKQGDRGQGEVALAPG
                     AVLWRGEPIARLEAPRGGIRPQPRLDPALERVPPAEREALAQALAAWLGRKLGPLAPL
                     ARLEEASRAEESGPELRALLIRLVEAGGVIARAGSGVDRLDKGRREGLRRLGVKVGAL
                     DLFVPAMVQPAALRLWAMLAGRDGPPPGHAAVLEAAAGTVPFGYRRCARQWLRVDVAE
                     GLLRGAHALRMAAGQRAFVIDPEPALAQGITTAALAHLLREAGFRASSARRLAPDEHG
                     P"
     gene            2619..4079
                     /locus_tag="VFF98_05280"
     CDS             2619..4079
                     /locus_tag="VFF98_05280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZU63074.1"
                     /translation="MDQLSSSAAALALPRARLFRAQPPRTGLAGWLEKRRIATDLARD
                     VGSAPWWRGLGLVMGCSAGALMLLPGLAPLPGLAASPLASAQLAEWRAGATRPLAMGG
                     AIGRIGLPGPFARPTDHAAERPRLDLVATLGEGDTLADALRRSGVGPADMAQALALVK
                     PALPAEGLAPGTRIAITLGPRADDNAPRRLDGLQLHAGFAEDLALSRQGGTLVLARHA
                     LAVDATPLHIRGVVGEGGLYRSARAQGVPAGAMQQYLEALDAHFSIDEVRPGDVFDLV
                     VTSQRAADGQSRLGNVLYAGLEQAGRPRTQLVRWGGEGQFFDALAPADTTASAGLVLP
                     VAGARITSSFGMRYHPILGYERMHSGTDLAAPWGSPVYAVADGYVEYAGWHGGHGNYL
                     RLAHGGGVETGYGHLSRYAVASGMRVRAGQVIGYVGSTGLSTGAHLHYEVFQSGRAID
                     PMSVHFIVRAGVDPREQAVFRARLAALLRLPVKTQG"
     gene            4153..5160
                     /gene="hemB"
                     /locus_tag="VFF98_05285"
     CDS             4153..5160
                     /gene="hemB"
                     /locus_tag="VFF98_05285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZU63075.1"
                     /translation="MTQTITAPFPALRPRRLRATAWSRALHRETVLTPADLIWPLFVT
                     EGQGVEEPIASLPGVSRWSVDGMVARAKEAAAAGIPCLALFPNTQPERRSHDGAEALN
                     PDNLMCRAIRAIRDALGDGIGILTDVALDPYTAHGQDGLIDEEGYVLNDATVEVLVGQ
                     SLNQAHAGADIIAPSDMMDGRIAAIRDALEGASLPNVQIMSYAAKYASAFYGPFRDAV
                     GSRGLLKGDKKTYQMDPGNTEEALREVAMDLAEGADSVMVKPGLPYLDIARRVKDQFG
                     VPVFAYQVSGEYAMIEAAVAAGAADRDAMVLETLLAFKRAGCSGVLTYHALHAARLLG
                     A"
     gene            5166..5756
                     /locus_tag="VFF98_05290"
     CDS             5166..5756
                     /locus_tag="VFF98_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZU63076.1"
                     /translation="MTIHSQDITIAPYDGVWPKIHSSAFIAPGCRIIGHVEIGPDVSI
                     WYNCVIRGDANRIRIGARSNIQDGTVVHCDSPKPRRPDGFPCLIGEDVLIGHMAMVHG
                     CTLEDRSFVGLGAIVMDGCTLETEAMLAAGALLSPGKVIPSRQMWMGRPAAFARTLND
                     EQVADMHAGVQRYVGYGRRHKESVAEGRVGYGAPER"
     gene            5737..6030
                     /locus_tag="VFF98_05295"
     CDS             5737..6030
                     /locus_tag="VFF98_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZU63077.1"
                     /translation="MARPSVDLPPADAVMALADDQGRFALRVTPGARVEGLAIDSGRL
                     VAKVRAKPEDGKANAAVIALLARALDVAPSALTLLRGATSREKQIGLVGGRRA"
     gene            complement(6027..6668)
                     /locus_tag="VFF98_05300"
     CDS             complement(6027..6668)
                     /locus_tag="VFF98_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZU63078.1"
                     /translation="MRHIDPERRLLAIALAALAGFVDASGFVGHGGYFLSFMSGNTTR
                     LAVDLAGAGPSALVPAGIIASFVGGVALGTAWADRAQGRKRKALLLVAVGLALWAAAW
                     ADGHGLPGPAIAAMASAMGALNTTFQRDGEVAIGLTYMTGALVKIGQGLGGWLGGHRR
                     AGWNGWAALWSALASGGLAGAVLALRAPWCTGWLAGGWALLAAAQAHRISRKT"
     gene            complement(6768..7184)
                     /locus_tag="VFF98_05305"
     CDS             complement(6768..7184)
                     /locus_tag="VFF98_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HZU63079.1"
                     /translation="MSDTQAPSPEAPAAPATPFDKLGGMPVLQAIADRFYDLMDSDPA
                     YAELRAMHAPDLAAMRRSLPGFLAGWAGGPRDWFEANPGKCMMSLHKPFTITRATAGQ
                     WAEAMRRAIEDVAPADQQLAQAMAGVLEEMALGMAR"
     gene            7327..8343
                     /locus_tag="VFF98_05310"
     CDS             7327..8343
                     /locus_tag="VFF98_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZU63080.1"
                     /translation="MRLFPALLRRAAAVLAMVAIAGQAASAAPRALPVGRCLNLGNHL
                     DTGDAQDKRLAAEDFHNIRAAGFTTVRLPVNWAKYSDPVGRGHGISPLWMARVSAMVD
                     AALDAGLNVILDSHHFDALHGDPAAASPWLAALWYQIGWQLADRPTTRLWFEIDNEPS
                     DRLTNANLMATLGPALAAIRQSNPDRPVLIGGENWSNVESLASLPLPADAHVYPTFHY
                     YTPMGFTHQGASWVRPPQPAGRSFGTPADMARLAADAERVRQFIARTGRVPVLGETGV
                     FTSVPLPQRAAYTRAVYQAFAPQGVGVCEWAYTNTFAFYDWRARQWLPGLLEAIGLKP
                     GKAR"
     gene            complement(8423..>8692)
                     /gene="clpA"
                     /locus_tag="VFF98_05315"
     CDS             complement(8423..>8692)
                     /gene="clpA"
                     /locus_tag="VFF98_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443729.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZU63081.1"
                     /translation="YGARPMARLIQEKVKKPLAEELLFGKLAHGGEVHVTVKPGEDER
                     PALAFELTPAAPKVVKPARGRKAASSAPEASGGKDEADESEGSAE"
BASE COUNT         1275 a         3132 c         2961 g         1324 t
ORIGIN      
        1 cggcccatgc tcatcaggcg ccagccgccg ggcagaggaa gcgcgaaagc ccgcctcgcg
       61 cagcaggtgc gccagcgccg ccgtggtgat cccctgcgcc agcgccggct cagggtcgat
      121 cacgaaggcg cgctggccgg ctgccatgcg cagggcatgg gcgccgcgca gcagcccctc
      181 ggccacatcc acccgcagcc actggcgcgc gcatcggcga tagccgaaag gcacagtgcc
      241 cgccgcagct tccagcaccg cagcatggcc cggcggcggg ccatccctgc cggccagcat
      301 cgcccacagc cgcagcgccg ccggctgcac catcgccggc acgaacagat ccagcgcccc
      361 caccttcacg cccagccgcc gcaaaccctc gcgccggccc ttgtcgagcc gatccacccc
      421 gctgcccgcg cgggcgatca ccccgcccgc ttccaccagg cggatcagca aggcgcgcaa
      481 ttccgggccg gattcctccg cgcggctggc ttcttccagc cttgcgagcg gcgccagcgg
      541 ccccagcttg cgccccagcc aggcagccag cgcctgcgcc agcgcctcgc gctcggccgg
      601 cggcacgcgc tccagcgcgg gatcgaggcg cggctgcggg cgaatgccac cgcgcggcgc
      661 ctccagccgg gcaatcggct cgccccgcca caacaccgcg ccgggcgcca gcgccacttc
      721 gccctgcccc ctgtcgccct gtttcgcgcc gcgccgcccg gccggctgct tcagcgcggc
      781 gatcaattcc tgcgcgcgca ccgccagcaa ttgcggcagg tgcttttcgg cggcggccag
      841 cagcagcttc tgatcggcca gccgcgccac cggatccacc cggaagcgaa agccgcgcag
      901 gctgcccagc ttctcgccct ccaccagcac atcctcgcct tccagcagca ccggcagcag
      961 cgccgcatcc ggccccagcc ggcgcatcag cacagtggtg cgccggttga cgaaacgctc
     1021 cgtcagccgc ccgtgcagcg catccgacag gcgcgcttcc accccccggg cgcgctcggc
     1081 catctcgctg cggttcagca cccagtcggg ccgctggcag atataggccc agctgcggat
     1141 ggcggcgatc cggccctgca gcgtgtcgat atcgcccgcc gtattgtcga gcgcgctgat
     1201 cgcccggggc gcatggtcgc tcgccagcgt gccgcgagac agttcctccc acagccgcgc
     1261 cacgaagcgg gcatgggttt cggccccctg gttgcggaaa tcgggcagcg agcagacatg
     1321 ccagaagcgg cggatctgcc ccggcaattg cagcccgcgc gcaatggccg gatcttccgc
     1381 caggatcttc agcacggcaa ggtcgatcgc ctgcggggcg ggcgccagtt cgggcagcag
     1441 gggcggcgct tccagatcgg cgatcagcct ggccagcgtg tcaaaacgcg gctccgcctc
     1501 gcgccagaac aggcgggtga ggggggcaaa gcggtgttcc tcgatggcaa agacctcgtc
     1561 ctcgccaaat tcggcatcat ggccgccgct gccggccagc gtgccaaagg tgccatcgcg
     1621 ctggtggcgc ccggcgcgcc cggcgatctg cgccatttcc gtgggcgtca gccgcctttg
     1681 ccgcacgcca tcgaacttgg agaggccggc aaaggccaca tgccccacat caaggttaag
     1741 ccccatgccg atggcatcgg tggccaccag ataatccacc tcgcccgcct gatagagcgc
     1801 cacttgcttg ttgcgcgttt cgggtgaaag cgcgcccatc accaccgccg cgccgccgcg
     1861 aaagcgccgc agcatttcgg ctacggcata aacctgctcg gccgagaagg cgacgatgac
     1921 gctgcgcggc ggaatgcgcg agagcttttt cgcgccggca tggctgagcg tggaaaagcg
     1981 cggccggctg atgatctcgg cctcgggcac cagcgccttc accatcggct ccatcaccga
     2041 ggagccgagc agcatcgtct cttcacgccc gcgcatgttg agcagcctgt cggtaaacac
     2101 atggccgcgc tcgcggtcgg cgcacagttg cacctcgtcc agcgccatga aggccagatc
     2161 ggcgcgggcc ggcatcgcct ctgcggtgca cagcagccag cgcgcgccgg gcggctcgat
     2221 ccgctcctcg ccggtgatca gcgccacccg ctcgcgcccc ttgatcgcgc agaccttctc
     2281 atacacctcg cgcgccagca gccgtagcgg aaagccgatg gcgccagagg aatgggcaca
     2341 caggcgctcg atcgccagat gggtcttgcc ggtgttggtg gggcccagca cggccatcac
     2401 gcggccatcg ccggaagggc cctgcgggcg ggcatgaggg cgcggggcag cccttggggc
     2461 gggggcatgt ctggccattg ccaagccaat ctggcatcgc ccatggcaga ctgcaaggcc
     2521 ccctgcggcc aacgggcgtt agcgcaggat taaccctgct ggtgcaccaa gggcatgaac
     2581 ggcccgcacc ccctgcgggt gcttcgggaa caccgcgttt ggatcagctg tcttcctctg
     2641 ctgctgccct ggccctgccg cgggcccggc tgtttcgcgc ccagccgccg cgcacggggc
     2701 tggccggctg gctggagaag cgccgcatcg ccaccgatct ggcccgcgat gtcggcagcg
     2761 cgccgtggtg gcgcgggctg gggctggtga tgggctgctc ggccggcgcg ttgatgctgc
     2821 tgccggggct ggcgccgctg ccgggcctgg ccgccagccc gctggccagc gcccagctgg
     2881 ccgaatggcg cgccggcgcc acccgcccgc tggcgatggg tggcgcaatc ggccggatcg
     2941 gcctgcccgg cccctttgcc cggcccaccg atcatgccgc cgaacgcccc cgcctcgatc
     3001 ttgtcgccac gctgggcgag ggggatacgc tggccgatgc cctgcgccgc tctggcgtgg
     3061 gcccggccga tatggcccag gcgctggcgc tggtgaagcc ggccctgccg gcagaggggc
     3121 tggcgccggg cacccgcatc gccatcaccc tgggcccgcg cgccgatgac aacgccccgc
     3181 gccgacttga tggcctgcaa ttgcacgccg gctttgcaga ggatctggcg ctcagccggc
     3241 aggggggcac gctggtgctc gcccgccatg cgctggcggt ggatgccacc cccttgcaca
     3301 tccgcggcgt ggtgggtgaa ggcgggctct atcgctcggc ccgggcgcaa ggggtgccgg
     3361 cgggcgccat gcagcaatat ctcgaagcgc tcgatgccca tttcagcatc gatgaagtgc
     3421 gccccggcga tgtcttcgat cttgtcgtca ccagccagcg cgcggccgat gggcaaagcc
     3481 ggctgggcaa tgtgctctat gccgggctgg agcaggccgg ccgcccgcgc acccagctgg
     3541 tgcgctgggg cggcgagggc cagttcttcg acgcgctggc cccggcagac accaccgcca
     3601 gcgccgggct ggtgctgccg gtggccggcg cgcggatcac ctcgtccttc ggcatgcgct
     3661 atcaccccat ccttggctac gaacggatgc attcgggcac cgatctggcc gccccctggg
     3721 gctcgccggt ctacgcggtg gccgatggct atgtggaata tgccggctgg catggcggcc
     3781 atggcaatta tctgcgcctg gcccatggcg gcggggtgga gacaggctat ggccatctca
     3841 gccgctatgc ggtggcctcg ggcatgcggg tgcgggcggg ccaggtgatc ggctatgtcg
     3901 gttccaccgg cctctccacc ggcgcgcatc tgcactacga agtgttccag tcgggccggg
     3961 cgatcgatcc gatgagcgtg catttcatcg tgcgcgccgg ggtggacccg cgcgagcagg
     4021 cggtgttccg cgcccggctg gcagcgctcc tgcggctgcc ggtcaaaacg caagggtaat
     4081 tgtgtttttt gcgcggcggg ctgctgggcg gccggccttt tcgcggggcg atcaaagggc
     4141 tagaagccgc gcatgaccca gaccatcacc gcgccttttc cggcgcttcg ccctcgccgc
     4201 ctgcgcgcca ccgcctggag ccgcgcgctg caccgcgaaa ccgtgttgac cccggccgat
     4261 ctcatctggc cgctgttcgt caccgagggg cagggcgtgg aagaacccat cgccagcctg
     4321 ccgggcgtgt cgcgctggtc ggtcgatggc atggtggcgc gggcgaagga agcggcggcg
     4381 gccggcattc cctgccttgc cctcttcccc aacacccagc cggaacggcg cagccatgat
     4441 ggcgccgagg cgctcaaccc cgacaatctc atgtgccgcg ccatccgcgc catccgcgat
     4501 gcgctgggcg atggcatcgg catcctcacc gatgtcgcgc tcgatcccta caccgcccac
     4561 gggcaggatg ggctgatcga cgaggaaggc tatgtgctga acgatgcgac ggtggaagtg
     4621 ctggtgggcc agagcctgaa ccaggcccat gccggcgccg atatcatcgc cccgtctgat
     4681 atgatggatg gtcgcatcgc cgccatccgc gatgcgctgg aaggggccag cctgcccaat
     4741 gtgcagatca tgtcctatgc cgccaaatat gccagcgcct tctatggccc gttccgcgat
     4801 gcggtgggca gccggggcct gctgaagggc gacaagaaga cctatcagat ggatccgggc
     4861 aacaccgagg aagccctgcg cgaagtggcg atggatctgg ccgaaggcgc cgacagcgtg
     4921 atggtgaagc cgggcctgcc ctatctcgac atcgcccgcc gggtgaagga ccagttcggc
     4981 gtgccggtgt tcgcctatca ggtgtcgggc gaatatgcga tgatcgaggc ggccgtggcg
     5041 gccggcgctg ccgaccgcga tgcgatggtg ctggaaacct tgctggcctt caagcgggcg
     5101 ggctgttcgg gcgtgctcac ctatcatgcg ctgcatgcag cgcgcctgct cggagcctaa
     5161 gcgccatgac cattcacagc caggacatca ccatcgcccc ctatgatggc gtgtggccca
     5221 agattcattc cagcgccttc atcgcccccg gctgccggat catcggccat gtggagatcg
     5281 ggccggatgt gtcgatctgg tataattgcg tgatccgcgg cgatgccaac cgcatccgca
     5341 tcggcgcgcg cagcaatatc caggatggca ccgtcgtcca ttgcgacagc cccaagccca
     5401 ggcgccccga cggttttccc tgtctgatcg gcgaggatgt gctgatcggc catatggcga
     5461 tggtgcatgg ctgcacgctg gaagaccggt ccttcgtggg gctgggcgcc atcgtcatgg
     5521 atggctgcac gctggagaca gaagccatgc tggcggccgg cgcgctgctg tcgccgggca
     5581 aggtgatccc ttcgcgccag atgtggatgg gccgcccggc cgccttcgcc cgcacgctga
     5641 acgatgaaca ggtggccgat atgcacgccg gtgtgcagcg ttatgtcggc tatggccggc
     5701 gccacaagga atcggtggcg gaaggccgcg tgggctatgg cgcgcccgag cgttgacctg
     5761 ccaccggccg atgcggtgat ggcgctggcc gatgatcagg gccgctttgc cctgcgcgtc
     5821 actcccggtg cgcgggtgga agggctggcg atcgatagcg ggcgcctggt ggccaaggtg
     5881 cgggccaagc cggaagacgg caaggccaat gccgcggtga tcgccctgct ggcccgcgcg
     5941 ctggatgtgg cgcccagcgc gctcaccctg ctgcgcggcg ccacctcgcg cgagaagcag
     6001 attggcctgg tgggcggccg ccgggcctag gttttccggc tgatgcggtg ggcctgggcg
     6061 gctgccagca gcgcccagcc gccggccagc cagccagtgc accagggcgc ccgcagcgcc
     6121 agcacggccc ctgccagtcc gcctgaagcc agcgcgctcc acagcgctgc ccagccgttc
     6181 cagcccgccc ggcgatggcc ccccagccag ccgcccagcc cctggccgat cttcaccagc
     6241 gcgccggtca tgtaggtaag gccgatcgcc acttcgccat cgcgctggaa ggtggtgttc
     6301 agtgccccca tcgcgctggc catggcggct atggccgggc ccggcagccc atgcccgtcg
     6361 gcccaggccg ccgcccacag tgccagcccc accgccacca gcagcagcgc cttgcgcttg
     6421 cggccttgcg cccggtcggc ccaggcagtg cccagcgcca ccccgcccac aaagctggcg
     6481 ataatgcccg ccggcaccag cgccgaaggc cccgcgcccg ccagatccac cgccagccgc
     6541 gtggtgttgc ccgacatgaa ggagaggaaa tagccgccgt ggccgacaaa gccgctggca
     6601 tcgacaaagc ctgccagcgc agcgagggca atcgccagca ggcgccgttc aggatcgata
     6661 tggcgcatgg gcgcagctta ggccaaccgg ccgttggcgg gcaagcgcgt ggtttatcgc
     6721 gcgatgcctc cccgcgtggt ttaccgcgcc atgccccccc gcgcggttta ccgcgccatc
     6781 cccagcgcca tttcctccag cacgccggcc attgcctgcg ccagttgctg gtctgccggg
     6841 gccacatcct caatggcgcg gcgcatcgcc tcggcccatt ggccggcggt ggcccgggtg
     6901 atggtgaaag gcttgtgcag gctcatcatg catttgccgg ggttggcctc gaaccagtcg
     6961 cgcgggccgc cggcccagcc ggccaggaag cccggcaacg aacggcgcat cgccgccaga
     7021 tccggcgcat gcatggcgcg cagctcggca taggcggggt cgctgtccat caggtcgtag
     7081 aagcggtcgg caatcgcctg caacaccggc atgccgccca gcttgtcgaa aggcgtggcg
     7141 ggcgctgcgg gggcttctgg ggaaggggct tgggtgtcag acaaaggggc atctcccggg
     7201 ggcggaattc ttcttgcagc tggctagcat gcaagggtca ggcaaggatt gatcatggtc
     7261 aagcgcgtgg caatcgcctg gcgcatcggc tagcgctgct ggcgccattt gccggggatc
     7321 tgttccatgc gcctgtttcc tgcccttttg cgccgcgctg ccgctgtgct tgccatggtg
     7381 gccatcgccg ggcaggcggc atctgccgcg ccgcgcgcgc tgccggtggg ccgctgcctc
     7441 aatctgggca accacctcga tacgggcgat gcgcaggaca agcgccttgc ggccgaggat
     7501 ttccacaata tccgcgcggc cggcttcacc accgtgcggc tgccggtgaa ctgggcgaaa
     7561 tattccgatc cggtgggccg cggccatggc atcagcccgc tgtggatggc ccgcgtctcg
     7621 gccatggtgg atgcggcgct ggatgccggc ctcaatgtca tcctcgattc ccaccatttc
     7681 gatgcgctac atggcgatcc ggcggcggcc agcccgtggc tggcggcgct gtggtatcag
     7741 atcggctggc agctggcaga ccggcccacc acccgcctgt ggttcgagat cgacaacgag
     7801 cccagcgacc ggctgaccaa cgccaacctg atggccacgc tgggcccggc gctggcggcc
     7861 atccgccagt ccaacccgga tcgcccggtg ctgatcggcg gcgagaactg gagcaatgtc
     7921 gaatcgctgg ccagcctgcc gctgccggcc gatgcccatg tctatcccac cttccattat
     7981 tacacgccga tgggcttcac ccatcagggc gccagctggg tgcgcccgcc acagcccgcc
     8041 ggccgcagct ttggcacccc cgccgacatg gcccgcctgg ccgctgatgc cgagcgggtg
     8101 cgccagttca tcgcgcgcac gggccgcgtg ccggtgctgg gcgagacagg ggtgttcacc
     8161 agcgtgccgc tgccccagcg cgcggcctac acaagggcgg tctaccaggc cttcgcgccg
     8221 cagggcgtgg gcgtgtgcga atgggcctat accaacactt tcgccttcta cgactggcgg
     8281 gcccggcaat ggctgccggg cctgctggaa gccatcgggc tgaagccggg caaggcgcgg
     8341 taagcgaaaa agggcgggtc aaaaaagggg cggcaggccc gaaagcccgc cgcccctttg
     8401 cgttttgcct tgcgggcagg ccttattcgg cgctgccctc gctttcatcg gcctcatcct
     8461 tgccgccaga ggcttccggg gcagaggatg ccgccttgcg gccgcgggca ggcttcacca
     8521 ccttgggcgc ggccggcgtc agctcgaagg cgagggcagg gcgctcgtct tcgcccggct
     8581 tcaccgtcac atgcacttcg ccgccatggg ccagcttgcc gaacagcagc tcctcggcca
     8641 gcggcttctt caccttctcc tgaatgaggc gggccatcgg ccgcgcgcca ta
//
LOCUS       DASRYE010000094         6384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_179965,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000094 DASRYE010000000
VERSION     DASRYE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179965"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="VFF98_05320"
     CDS             <1..1805
                     /locus_tag="VFF98_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63082.1"
                     /translation="QPSVRVYQDGVLLLGGGAYFYDLDRVEVLRGPQGTLYGRGAVAG
                     AVNIVSRKPVFQNEGFAQLGLANYGQIDAQAMINAPLSDKLAVRVAGNFLRHDGYFNN
                     GQSSASEGSVRGQMLFKTEDVSVLLAATHYQNDSTAPGKVIVTASDPYVHGWTTPEAP
                     GGYTKGQYTEVHGQLDWKINGVTLTYIPSYATFDSYSVQLLQYGRYNGKEIGNQPYHR
                     VHTQELRLAHEAGGLQVQGGLFYYNEQYAYQYLNLAPNSAAGTGMDAYGYNAASNGTH
                     QVFDLTSAAIFAQGTYALADALRLTLGGRYTYDKVNQLYEGNHQDITPGSNPVVAPFD
                     EHWTHFDWKVRIEGDLTPRNLLYASVTTGYRPGTSTIGVAFQPETVTTYEAGSKNSFL
                     HGALVANLSGFYSHYPVYQVVQTYTLANGDLRAYIPQVPATFYGLEAELIARPTRHDT
                     LNLSGSWLRATFDGNLTSVNPTTQVATTYLTKGKALPHAPTWHVTAAYQHLFPLRSGA
                     EIALSGDLTYQSAQTTDYDGSNYPAPNPLYLQPSTTEYNASLTFSSPSKRYRLTVYGK
                     NLGNIIYKLQANVAPGVEYVNDPRSFGARFSAKF"
     gene            complement(1881..3062)
                     /locus_tag="VFF98_05325"
     CDS             complement(1881..3062)
                     /locus_tag="VFF98_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU63083.1"
                     /translation="MAGLAMSPVLDNVAQAGRGRPDLLRLNTLLLHLYDQPHGTRAGW
                     WSCIEHLRKHFGAEQAAFIIRRRLSGDPTELIISGEEPCPEARQMFLDRYSAIDPFSN
                     LPPERACTVQDILSPDEWRASEFFRKYLSPQRLVQVMGADIDISRERVMAFRICRSPG
                     AGEFSDQDREEFQFLLPHLRRIAETRIRHSTLEASDRIFTDLLDCMELGAIVLDQAGE
                     VLKINPTAERMLARREGVTLANGALRALNAEDNRHFLKLIRQALASSRQGGELIGTIG
                     LKRRENEQLLGVAVRSLASEGAVTGIRQATVAVLLGGGRGHEHISESALKSLFSLTNA
                     ETRLAIALMRGATIDEVAGQMGVSRNTIRTHLNGLFLKTGTNRQSEVIRALLGSIASI
                     C"
     gene            3281..3565
                     /locus_tag="VFF98_05330"
     CDS             3281..3565
                     /locus_tag="VFF98_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63084.1"
                     /translation="MSIFAVEQVMWDVSRDGRMAEACRTDPGDFLAGYRLDAQEAQAI
                     ASLDIAGLSAWGINPMLLMQAWNALVGPDAIDEYLARMNGAGQARKADHG"
     gene            3558..4391
                     /locus_tag="VFF98_05335"
     CDS             3558..4391
                     /locus_tag="VFF98_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HZU63085.1"
                     /translation="MAELVAGFLLPHVPLIKSSTAAAPAGQRSRVFAAFDEISALLRA
                     RAVDTVIIIGSDHCSLFGSECLPPCLIGTGDVRGPLEDWLGLERQDLPSHPALARHII
                     DTGLVDGIDWSVSRNLLVDHAIMIPFHYVVRPNPGQRIIPVYLNAGGDPALPSWRAAR
                     MGESIAAAVRSWPGDERVAILGTGGLSHWVGMARMGTVNEAWDRRIIALEEAGDLAAL
                     IAIADADILAEAGNGALEIKNHICAMAAMPGSRARLIAYEPVPAWISGCGFMELQSAA
                     A"
     gene            4608..5909
                     /locus_tag="VFF98_05340"
     CDS             4608..5909
                     /locus_tag="VFF98_05340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU63086.1"
                     /translation="MALSRPLAAAEGCVFLIEDSVALTLLLKERIESATSCPVHGYKS
                     YAEAREALKSRRPALAIAGVDLPDAPRGEILDLLADHDIPTILFTTIIDEKIRQRMAR
                     PNMLDYLVKGPGESVEKIIALVGQATQGHAIPVLLVDDSSSERAALAAILRRQNCQVF
                     EAAAASEALEILTQHEDIELILTDYNMPDMDGQHLTRRIRQMRPADRVRVIGISSSSD
                     RGLSAAFLKAGAADFIYRPFIDEEVQCRVAHNIETLQQIKRLRYLAERDLLTGLFNRR
                     AFFEHAYAALEGLPGGDARGWIGILDIDHFKQVNDTYGHDAGDKVLKEVAGVLAAVAG
                     REGFLAARFGGEEFILMLLRRDHGQVMALCEEVRSRIAALTIPYEASAVHVTASIGLA
                     GIQQGESLDNNLNAADQMLYMAKAGGRNQVVSDAALYGLAG"
     gene            complement(5929..6189)
                     /locus_tag="VFF98_05345"
     CDS             complement(5929..6189)
                     /locus_tag="VFF98_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63087.1"
                     /translation="MDFDDQMRRYFGAAELGEVTPAVLASGMERLEVDFGLEKDRGRR
                     FAMWALMHILGNAPDLDVAFKDPADQDAARDFMDMLARLNEA"
     gene            complement(6248..>6384)
                     /locus_tag="VFF98_05350"
     CDS             complement(6248..>6384)
                     /locus_tag="VFF98_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZU63088.1"
                     /translation="GKFKVKQSPAREGRNPSTGATIQIAASRKLGFAPAKAVKDKING
                     "
BASE COUNT         1118 a         1999 c         2104 g         1163 t
ORIGIN      
        1 agcagccctc ggtgcgcgtc tatcaggatg gcgtgctgct gctggggggc ggggcctatt
       61 tctacgatct tgatcgggtg gaagtgctgc gcgggccgca gggcacgctg tatgggcgcg
      121 gggcggtggc cggggcggtc aatatcgtct cgcgcaagcc ggtcttccag aacgagggct
      181 ttgcccagct gggcctggcc aattacggcc agatcgatgc ccaggcgatg atcaatgccc
      241 cgctgagcga caagctggcc gtgcgggtgg cgggcaattt cctgcgccac gacggctatt
      301 tcaacaatgg ccagagctct gccagcgagg gcagcgtgcg cgggcagatg cttttcaaga
      361 ccgaggatgt gtcggtgctg ctggccgcca cccattacca gaatgacagc acggcaccgg
      421 gcaaggtgat cgtgaccgcc agcgatccct atgtgcatgg ctggacgacg ccggaagcgc
      481 cgggcggcta cacgaagggg caatataccg aagtgcatgg ccagctggac tggaagatca
      541 acggcgttac cctgacctat atcccgtcct atgccacgtt cgacagctat agtgtgcagc
      601 ttctgcaata tggccgatat aatggcaagg aaatcggcaa ccagccctat caccgggtgc
      661 atacgcagga actgcgcctg gcgcatgagg ccggtgggtt gcaggtccag ggcggccttt
      721 tctactataa cgagcaatat gcctatcaat atctcaatct ggcgcccaat tcggctgccg
      781 gcaccgggat ggatgcctat ggctacaacg ccgcttccaa cggcacgcat caggtgttcg
      841 acctgacctc ggcggcgatc ttcgcgcaag gcacctatgc ccttgccgat gctctgcgcc
      901 tgaccctggg ggggcgctac acctacgaca aggtcaacca gctttacgaa ggcaatcatc
      961 aggacatcac gccgggcagc aatccggtcg tggccccctt cgatgaacac tggacccatt
     1021 tcgactggaa ggtgcggatc gagggcgatc tcacgccccg caacctgctc tatgcctcgg
     1081 taacgaccgg ctatcggccg ggcaccagca ccatcggcgt ggccttccag cccgaaacgg
     1141 tgacgaccta tgaagcgggc agcaagaata gcttcctgca tggcgcgctg gtggccaatc
     1201 tttcggggtt ctacagccat tatccggtct atcaggtggt gcagacctat actctggcca
     1261 acggcgatct cagggcctat atcccgcagg tgcccgccac tttctatggc ctcgaggccg
     1321 agctgattgc ccggcccacc cggcacgata cgctcaacct gtcgggatcc tggctgcggg
     1381 cgacgttcga cggcaatctg acctcggtca acccgacgac gcaggtggca acgacctacc
     1441 tcaccaaggg caaggcgctg cctcatgcgc caacctggca cgtgacggcg gcctatcagc
     1501 atctcttccc gctgcgcagc ggcgccgaaa tcgccttgtc gggcgatctg acctatcaga
     1561 gcgcgcagac caccgattat gacggcagca actatcccgc ccccaatccg ctctaccttc
     1621 agccatcgac gacggaatac aacgcctcgc tgaccttctc ttcgccgagc aagcgttaca
     1681 ggctgacggt ttacgggaaa aatctgggca atatcatcta caagctgcag gccaatgtgg
     1741 cccccggggt ggaatatgtg aatgatccgc gcagctttgg cgcgcgtttc tcggccaaat
     1801 tctgatccgg cggcgcgagg acggaagaag agggggcggt gcgaggggcg ccgctccctt
     1861 tcttgtgccc agcacagcag tcagcagatc gaggcgatgc tgcccagcag ggcacgaatg
     1921 acctcgctct ggcggttggt gccggtcttc aggaacaggc cgttcagatg ggtgcggatg
     1981 gtgttgcggc tgacgcccat ttgcccggcg acctcatcga tcgtggcccc gcgcatcagc
     2041 gcgatcgcca ggcgggtttc ggcgttggtc agggaaaaga ggcttttgag cgcgctctcg
     2101 gagatatgtt cgtggccccg gccgccgccc agcagcacgg caacggtggc ctggcggatg
     2161 ccggtgaccg cgccctcgct ggccaggctg cgcacggcca cgcccagcaa ctgctcgttt
     2221 tcgcggcgct tcagccctat cgtgccgatc agctcgccgc cctggcgcga ggaggccagc
     2281 gcctggcgga tgagcttgag gaaatggcgg ttgtcttcgg cattgagagc gcgcagcgcg
     2341 ccattggcca gggtcacgcc ttcgcgccgg gccagcatgc gttcggcggt ggggttgatt
     2401 ttcagcactt ccccggcctg atccagcacg atcgcgccca gctccatgca atccagcaga
     2461 tcggtgaaga tccggtcgct tgcttccagc gtggagtgcc tgatccgggt ttccgcgatc
     2521 cggcgcagat gcggcagaag gaactggaat tcctcgcggt cctggtcgga aaattcgccg
     2581 gcgccggggg agcggcagat gcgaaacgcc atcacccgct cccgcgagat gtcgatgtcg
     2641 gcccccatca cctgcacaag cctttgcgga gacaggtatt tgcggaaaaa ttcgctggcc
     2701 cgccattcat ccgggctgag gatatcctgc accgtgcagg cccgctcggg gggcaggttc
     2761 gagaagggat ctatcgccga atagcgatcg aggaacatct gccgcgcttc ggggcacggc
     2821 tcctcgccag agatgatcag ttcggtggga tcgccggaaa ggcgccggcg gatgatgaag
     2881 gcggcctgct cggcgccaaa atgcttgcgt agatgctcga tgcagctcca ccagcccgcg
     2941 cgcgtgccat ggggctgatc gtaaagatgc agcagcaagg tgttgaggcg cagcaggtcg
     3001 ggcctgccgc gacctgcctg cgccacattg tcgagcaccg ggctcattgc cagtcctgcc
     3061 atgccatctc tccctttgcg gatatcccgg gttggcctgc ggcctttttc ccgggattcc
     3121 attttttatg attcatgcaa gattttcgat aatctgaata ttggcgtaat atatgtcaat
     3181 cgagagatgg aggtggcgga tcatccgatc ggatgaggcc gcaggggatc ggcaggggct
     3241 agtcaggcag ccggcatcag ggccgggcaa ggagggcgcg gtgagcattt tcgcggtcga
     3301 acaggtgatg tgggatgtct cgcgcgatgg gcgcatggcc gaggcatgca ggacggatcc
     3361 gggcgatttt ctcgctggct accggctcga tgcgcaggag gcgcaggcga tcgcctcgct
     3421 cgatatcgcc ggcctgtcgg cgtggggcat caacccgatg cttctgatgc aggcctggaa
     3481 cgcgctggtt ggccccgatg cgatcgatga atatctggcg cggatgaatg gcgctggaca
     3541 ggcaagaaag gcagatcatg gctgaactgg tggccggttt tcttctcccc catgtgccgc
     3601 tgatcaaatc cagcaccgcg gctgcccctg ccgggcagcg cagccgggtt ttcgccgctt
     3661 tcgacgagat cagcgctttg ctgcgcgcgc gcgcggtcga cacggtgatc atcatcggca
     3721 gcgatcattg cagcctgttc ggttcggaat gcctgccgcc ctgcctgatc ggcaccggcg
     3781 atgtgcgcgg gccgctggaa gactggctgg ggcttgaacg gcaggatctg cccagccacc
     3841 ccgcgctggc ccggcatatc atcgacaccg gccttgtcga cgggatcgac tggtcggtct
     3901 cgcgcaacct gctggtggat catgcgatca tgatcccctt ccattatgtt gtgcgcccca
     3961 atcccgggca gcggatcatt ccggtctatc tcaatgccgg gggtgatccg gcgcttccgt
     4021 cgtggcgggc ggcgcgcatg ggcgaaagca tcgcggcggc ggtgcgctcc tggccgggcg
     4081 acgagcgtgt ggccatcctg ggcacgggcg ggctgagcca ctgggtgggc atggcccgca
     4141 tgggaacggt gaacgaggcg tgggaccggc gcatcatcgc gctggaagaa gccggcgatc
     4201 tggcggcgct gatcgccatc gccgatgccg atattctggc cgaggcgggc aatggcgcgc
     4261 tggaaatcaa gaaccacatc tgcgccatgg ccgccatgcc cggatcgcgc gccaggctga
     4321 tcgcttacga gccggtgccc gcatggattt ccggctgcgg cttcatggaa ctgcaatccg
     4381 cggctgcatg acctgcgccc cggcgcaggt tttggccggc ccggcgctgg ccggccgctg
     4441 ccgcccatgc cgtttgtgcg gagatgtttg ggagcggcct agggaagttt cctgattatg
     4501 gcgcatgcca ttgccttttc cgttccctgc taggcacggc tgccggctgg ccgatcgggg
     4561 cgtgggagaa tgggtggcgc atgagttgca tcaagcagca ggatcgcatg gccttgagcc
     4621 gccctttggc ggcggccgaa ggctgcgtct tcctgatcga ggattcggtg gcgctgacgc
     4681 tgctgctgaa agagcgcatc gaaagcgcca catcctgccc ggtgcacggc tacaaaagct
     4741 atgccgaagc ccgcgaggcg ctgaaaagtc gccgcccggc gctggcgatt gccggcgtgg
     4801 atctgcccga tgcgccgcgg ggggaaatcc tcgacctgct ggccgatcac gatatcccca
     4861 ccattctctt cacgacgatc atcgacgaga agatccgcca gcgcatggcc cggcccaaca
     4921 tgctggacta tctggtcaag ggcccgggcg aatcggtcga gaaaatcatc gccctggtgg
     4981 gccaggccac ccagggccat gccatccccg tgctgctggt agacgacagc agcagcgaaa
     5041 gggccgcgct ggcggccatt ctgcggcgcc agaattgcca ggtgttcgaa gccgcagcgg
     5101 ccagcgaggc gctggagatc ctcacccagc acgaggacat cgagctgatc ctcaccgact
     5161 acaacatgcc ggacatggat ggccagcatc tcacccggcg catccggcag atgcggccgg
     5221 ccgacagggt gcgggtcatc ggcatctcct cctcatcgga tcgcggcctg tctgccgctt
     5281 tcctgaaggc cggcgcggcc gatttcatct accggccctt catcgatgag gaagtgcaat
     5341 gccgcgtggc ccacaacata gagacgctgc agcagatcaa acgcttgaga tatctggccg
     5401 agcgcgacct gctgaccggg ctgttcaacc gccgggcctt tttcgaacac gcctatgccg
     5461 cgctggaagg cctgccgggc ggcgatgccc gcgggtggat cggcattctc gacatcgacc
     5521 acttcaaaca ggtcaacgac acctatggcc atgacgcggg cgacaaggtg ctgaaggaag
     5581 tggccggcgt gctggcggcg gtggccggcc gcgagggctt tctggccgcg cgcttcggcg
     5641 gcgaggagtt catcctcatg cttttgcgcc gcgatcacgg gcaggtgatg gccctgtgcg
     5701 aggaagtgcg cagccgcatc gcggcgctca ccatccccta tgaggccagc gcggtgcatg
     5761 tcaccgcctc gatcgggctg gccggcatcc agcagggcga aagcctcgac aacaacctca
     5821 atgcggcgga tcagatgctg tatatggcca aggcgggcgg gcgcaatcag gtggtcagcg
     5881 atgccgcgct atacgggctg gcgggctgag gcgctgttct gcccggcgtt atgcctcatt
     5941 caggcgggcg agcatgtcca tgaaatcgcg cgcggcgtcc tgatcggccg ggtccttgaa
     6001 ggccacatcc agatccggcg cattgcccag gatatgcatc agcgcccaca tcgcgaagcg
     6061 gcggccgcga tccttttcca ggccgaaatc aacctccagc cgttccatgc cgctggccag
     6121 cacggcgggc gtcacctcgc ccagctcggc cgcgccgaag tagcggcgca tctgatcgtc
     6181 gaagtccatg cgggcctgct ttcgaatggg gaaggcggcg cccggccggg ccaggcgccg
     6241 gccggcatca gccgttgatc ttgtccttca ccgccttggc cggggcaaag cccagcttgc
     6301 gcgaggcggc gatctggatg gtggccccgg tggaggggtt gcggccttcg cgcgcggggc
     6361 tttgcttgac cttgaacttg ccga
//
LOCUS       DASRYE010000095        15800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_5726,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000095 DASRYE010000000
VERSION     DASRYE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15800
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5726"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFF98_05355"
     CDS             complement(<1..685)
                     /locus_tag="VFF98_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63089.1"
                     /translation="MKLLVTGREGQVVRSLLERAGAGVQVEALGRPALDLAEAAGIAG
                     AIAASFGGSGPDVVIHAAAYTAVDRAEGEEALALAINGAGAGAVAAAAQELGAQLIHI
                     STDYVYDGAKPAPYVESDACAPQGAYGRSKLAGEAAILAAHPGAVIARTAWVYSPFGA
                     NFVKTMLRLASTRAEVGAEVSVVADQLGCPTNALDMADALLVMAARLRADPSAALRGV
                     FHVAGSGEGS"
     gene            complement(682..1740)
                     /gene="rfbB"
                     /locus_tag="VFF98_05360"
     CDS             complement(682..1740)
                     /gene="rfbB"
                     /locus_tag="VFF98_05360"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZU63090.1"
                     /translation="MRTIVTGGAGFIGSALVRHLVLEQQEEVLTIDALTYAGNLASLQ
                     AVEGCANHRFLKADIRDGAAMAAAIASFAPTRILHLAAESHVDRSITGAADFIHTNVV
                     GTFTLLEAARAYWAGLPAAQQAAFRFLHVSTDEVYGSLGAEGLFREDTPYDPSSPYSA
                     SKAASDHLAKAWARTYGLPVVVSNCSNNYGPYHFPEKLIPLTILNALAGRELPVYGTG
                     ENIRDWLYVEDHARALDLIARQGRVGETYNVGGRNERRNIDVVRRICAVLDALAPAAA
                     PREALIRFVTNRPGHDARYAIDATRLETELGWRAQENFDSGIEKTVRWYLDNGWWWQP
                     LRDRYDGARLGLVAEKQA"
     gene            complement(1737..2309)
                     /gene="rfbC"
                     /locus_tag="VFF98_05365"
     CDS             complement(1737..2309)
                     /gene="rfbC"
                     /locus_tag="VFF98_05365"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZU63091.1"
                     /translation="MEFRTFPIAGVIEIVPRRHGDARGYFSEIFREDAFRAAAGDVGF
                     VQENQSLSAKAGTIRGLHFQTAPAGQGKLVRCVAGAILDVAVDIRHGSPTFGQWVAAT
                     LSADNGHQLWVPEGFAHGFCTLLPDTVVCYKVTAYYSPEHDKGLAWNDPAIAIAWPDV
                     ADAETLSAKDRVQPLLADLPALFPLAGAGA"
     gene            2523..3788
                     /locus_tag="VFF98_05370"
     CDS             2523..3788
                     /locus_tag="VFF98_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HZU63092.1"
                     /translation="MPAEPARRFLFLQGPPGPFFRLLGAKLAAAGATCLRLNLNGGDA
                     HDWPRLADGRRTPAWRGPAHRWPPFIEAFLRDHAITDLVLFGDCRPLHMVARQMAQLA
                     GIRVHVFEEGYIRPNWLTLEQGGVNGHSPLLRDAAALLAAGTCLPPPPDLPPIGAHMG
                     RRVADSWGYFGHMLLRAPLYPFHRSHRPGSIVLEGLGWALKYLRRAARQRQTERALAG
                     LAGRPYFLFPLQLSADYQIRAHSPFAHMAQAADHVLASFAAHAPADALLVVKDHPLDA
                     TLAGWGGFLARRARALGLAARIIHVPGGDLAALALMARGVVVVNSTAATFALAEGVAV
                     KALGTALYDLPGITHQGPLASFWAAPQAPDPQLYGSFCRVLHRHCLVRGGLASRSAIA
                     ILVQNAAARLLAPTLAQTAQPRSPIPAAA"
     gene            complement(3808..4173)
                     /locus_tag="VFF98_05375"
     CDS             complement(3808..4173)
                     /locus_tag="VFF98_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HZU63093.1"
                     /translation="MIGQDEARRGGWTGPGDPHTPPLCCGAAWSVNGKIIVNERFLPL
                     LGWAASIMAVAMYLAYIDQIRLNLDGHKGSIIQPLAAVANCSLWSLYGFLRKRREWPI
                     VAANLPGIFFGLAALLTAI"
     gene            complement(4201..5376)
                     /locus_tag="VFF98_05380"
     CDS             complement(4201..5376)
                     /locus_tag="VFF98_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU63094.1"
                     /translation="MGFAQAAKGCGGQRVAVLALLLGTGGCAALPVSGPTGHEVLREA
                     GGGPGQGAAAGLPFQVVELANFASLPPAPPAPPVEQPAGAPLPTDLIGPGDVLDIAIY
                     EAGVTLFAGARGGDAQGAGLTGGAQAERLGGIRVDDAGLVRLPYAGPIRAAGRTPGEL
                     ALQIRAGLKGLSQNPQVVVAISQTIANSVIVGGEVGRPGRLVLATNRETLSDAVALAG
                     GYRGEAKDLAVRVVRAGRALEYRLADVLSAPARDMRVAPGDRIEVLRQPFSFAVMGAA
                     GKVEQMAFSGPGVSLAEAVAMAGGAHPALGDAKAVFVFRFVADGAGGEKPVVYHLDMM
                     RGGAYFLSQRFAMRDKDLLFIGNAAANQPTKLIQLVSQLFTPIVAVESGLVTTGALR"
     gene            5560..6486
                     /locus_tag="VFF98_05385"
     CDS             5560..6486
                     /locus_tag="VFF98_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63095.1"
                     /translation="MALIPPSRLAAIPHLGTFLAGHERAVVGWGRKPSGRRAQRLAGW
                     LGRPVALLEDGLLRSVGRGDPPLSLLVDDIGVYYDAAAPSRMEHSIAAGASPRQAARA
                     LALAAAWRAGGLSKYNHAPDPAALPTGPYVLLADQCRGDLSIAGAQACAASFARMAEA
                     AAVEFPHHALVLKTHPDAHSHGRASCLPPALGAHPRAVPVADGCHPARLIAGAAAVYT
                     VSSQIGFEALIWGRPLRCFGLPFYAGWGLAEEDRPPPARRAAGARLEDLLHAALVVLA
                     RYVDPASGRAWQAEEAFAHVASARRQRARPAG"
     gene            6490..7518
                     /locus_tag="VFF98_05390"
     CDS             6490..7518
                     /locus_tag="VFF98_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63096.1"
                     /translation="MAGRPTTDLYFRNALAPFIAPRWWPVGWPAGQAAPIRACGAHSF
                     SAGRWRVLLREDAPAAMRAALDHAGPLAYLVDDDFAAAASCPALPEAYRQRLAAFARG
                     WHAALLARADVVLAASAPLAARLAGQPGLAPRLRLIEPFWRESPADQAHFAPLTGGAP
                     LRIAHLGSASHGAALAALAPALAALLDRHPACQFTYFARAPVDPALEAHPRARRQQPM
                     AWPEYRRWLARQRFHLALYPLLDNGFDRARSASKLAEHAVAGAVGVYPAGWAPGAALA
                     AAGGALLAPDRVELWPAAMDRLIGQIMQKHHKLAQIAAQAAKTLSINDPCAKQRQIWS
                     NSLDLCLA"
     gene            7515..9290
                     /locus_tag="VFF98_05395"
     CDS             7515..9290
                     /locus_tag="VFF98_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU63097.1"
                     /translation="MNFTSAFVRTVFWRPGRALSALYWQLTGRRKRARNRLRLEMAQS
                     PRAYARWIASVERQEDVLAAAPDRIAQWPTRPLISVVLHLEAGSRQGQVERCLAALRA
                     QCYPEWELVLVQSYDAPPTRSIDVPRLLLAPSRAGSRAQALALGAECARGSHILPIRA
                     EHVLAPTALLHLATALQAQSPAPHLLYGDHDRVDRAGRRHSPWFKPEWNAELALGQDY
                     VSQACLIRRDAARACLPIPPALADAATYALVLAVSALAEAQIVHVPHMLCHCTSGESV
                     NEPAARAAVVERHLAGSGASLRLGSHGTVQVHWPLPDPAPLVSILVPTRDRLDLLRAC
                     ITGVLNATRYPAFEVLIIDNGSQDPATLDYLARIAGNPRVRVLPWHRPFNYSAINNFA
                     AREARGRYLCLLNNDVDVVDDQWLPALMRQAARPHVGAVGAKLLYADGRIQHAGVVVG
                     LGDAAGHAHRFQRDSDCGYFARPHVAHYVSAVTGACLLVEKAKFEAVGGLDEEAFPVA
                     FNDVDLCLKLAAAGYRNVYVPQAVLVHHESASRGHDSLAANRARYAGELAALRARWHT
                     ESFLDPLHHPELERSSETYLIRQPA"
     gene            complement(9287..9934)
                     /locus_tag="VFF98_05400"
     CDS             complement(9287..9934)
                     /locus_tag="VFF98_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU63098.1"
                     /translation="MIRCTGLAKHYPQGRARKQVLAGLDLVVRPGQRLGILGRNGAGK
                     TTLIKLIGGVELPSSGVVERQMSLSWPLGFGGGFQGSLTGFDNARFIARIYGRDYADI
                     RGFVEDFSELGRQLAMPVKTYSAGMKARLAFALSLAIEFDCYLIDEVIMVGDQNFQRK
                     CMAELFEKRADRALILASHAPEMIRRLCDSALLLEGGHGRLFADVEEGLAAYEAL"
     gene            complement(9931..10746)
                     /locus_tag="VFF98_05405"
     CDS             complement(9931..10746)
                     /locus_tag="VFF98_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63099.1"
                     /translation="MAVSHTESHTGARLAAGWRIQRRVIAALMIRELTTRFGRENIGF
                     LWVMAEPLLFATLVGLLWRIMHGVSEHGINVFAFVATGYLPMVLFRNAVNRSVGLFQA
                     NASLMYHRQILIQDFVLVRFCVECIGHMMAYLFIGVVMVALGLFPMPYDLALMLAGWA
                     VYGWFTLAMCCLLAPLSELSPVLEKVMPVSVYLMVPFSGAFTMAAWLAPGARALLLCS
                     PPVHGMEMMRAGLFGPAHQAYYALFYPLAVSGAIMAVGLWLCRQVRRHLVVEA"
     gene            complement(10750..11958)
                     /locus_tag="VFF98_05410"
     CDS             complement(10750..11958)
                     /locus_tag="VFF98_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HZU63100.1"
                     /translation="MTQMVVQEMRRMFTPADGADGGAGPAAVAAGRRRAWPGGLWRHR
                     WMALVVGVPTLLSAIYFGGFAADEYVSQARFVIRGAGQKSAQIATLANLVQTTTPGTA
                     HEQASEVVDFLRSRSALAELGGKADLRAAYGHGGDWLGRYPGPFQADTFEGLYRYYTG
                     KVSARIDPESGIAVLEVRAYRAGDAQAINLALLGAGEGLVNRLNQRAEARAIAESERR
                     VALAEQRLARARLALGAYRNAQALIDPARQAGGALDVSNRLEAEQAALAAQLQLMEKA
                     APANPAIPALRARVAAMAAQAGLQEGRAVGGGGAIAAKLAGYDRLALEQEFATQALAA
                     ANTALEQARSDAARQQFYLERVVEPNRPDWPALPHRWRIVLTVLASSLCLYAIGWMFL
                     VGLLEHAPEE"
     gene            complement(11955..14426)
                     /locus_tag="VFF98_05415"
     CDS             complement(11955..14426)
                     /locus_tag="VFF98_05415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU63101.1"
                     /translation="MSVIDRAADVPAGFDAAWYAARFPDVGLSGLAPAEHYRLIGARI
                     GRPAGPQAALARRPAAGRAEVPFRSFGIIRHCPLISVVIVSFNSGKDLAALLPTLAAQ
                     SWAPLEVIVVENGMEETEALCRAHIPDCRWLRADNPGFAEANNIAVELARGEFIALVN
                     PDTVLAPDCLQQLVEALRMDASAAVAVPKIDFFTPFLRLSVEADAPFSLARADLLAGL
                     DYAKLFVRNGRQEGAQIHSDGDGRLEVDLPHARRALRLELAGGRGLRQARVGLGHAPV
                     ASFVAAPAGRVVACLPYEARHAASARPLVNNAGSGLRADGTPYDRGFGEEDDGRFFSK
                     AYVEALCGCMALVRRVAVMERKLFAPAFFAYFEDSELSAWLRGRGYRILYQPAARVAH
                     RHSESTEEHSPVWRTLVARSGDLYRRLTGGAPLADFAPHYEAGLPPALRQRLEAYDDD
                     VRAAGGGAALVAPGRLKICIYNSYFASMGGGEKHAIDIACALAGLGEVYLASESDFDM
                     AGLEAYFGVDLAHVRKLVSTEVDTHFTAKFDVFINATFRSNLVPAAGRNIYVVSFPHR
                     DVDPAILPAYTFLHNSPYTRDWAGRLWGAHDGRVLLPILGFAGGGARADAKRRQILTL
                     GRFTYDGHCKNHHQILRAFLDVRRRHRDLADWELVVIGSFEASQPSAARYLADLQALA
                     AGAPVQIIANATRAQVAQACAASAIYCHATGLGMPDAAPEKQEHFGIAPYEAMLAGCA
                     PVVHARGGPAAQVAGLADGWTFADEAGLARALAGAMRRHAAGGVDAGAIAAHARALQA
                     GNGAVLAALIDEMRAGAPAPTGGRG"
     gene            complement(14423..>15800)
                     /locus_tag="VFF98_05420"
     CDS             complement(14423..>15800)
                     /locus_tag="VFF98_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63102.1"
                     /translation="RAGVDLGLGWPRLSASTAEEIGAFLAADLRHHQVPAGQVLDNPL
                     ICGWVREAYAILLRWAAGEEAAADYARLDAIRVAFDEAGEGFAGLVARGQAGLLRAGA
                     LAGELAADRALHARAAEEEARARTALAAELAADLAADLARAQAEAQAREGAIALRLAA
                     AEVALAERGRDAEVLAGQLAEAESALRDSRAEAAAAASALAGQAAQLNTARRALAAAE
                     AREQQRFGTLLRAKEELAGALRGLARARAAAAEVAPLQAALAEARGQAAQQAQALEAA
                     LAGQAALASARDAAQQALAQQQAQAVVQAAQQAQAAQAAEAAQAALLADATRAAEEAA
                     QAARARLAELEENLAARFHEMAAMARLLQQAEAAAAARAEEANWLRGLGAVLTCRAGW
                     EGWLPPPLRLRRQHQRLAGAGLFDAAAYLAAHPDVRAAGAEPLRHLLQHGLAEGRCVP
                     GRHEGGAA"
BASE COUNT         2437 a         5962 c         5110 g         2291 t
ORIGIN      
        1 agctgccctc gccgctgccc gccacatgga acacgccgcg caacgccgca gacggatccg
       61 cccgcagccg cgccgccatc accagcagcg catcggccat atccagcgca ttggtggggc
      121 agcccagttg atcggccacc acgctcactt ctgcgcccac ttccgcccgc gtgctggcca
      181 ggcgcagcat cgtcttcacg aaattggcgc cgaacgggct gtagacccag gccgtgcggg
      241 cgatcaccgc gccggggtga gcggccagta tggcagcctc gccggccagc ttgctacggc
      301 cataggcacc ctgcggcgcg caggcatcgc tttccacata gggggccggc ttggcgccat
      361 catagacgta atcggtggag atatggatca gctgcgcgcc cagctcctgc gcggccgccg
      421 ccaccgcgcc cgcgcccgcg ccattgatcg ccagcgccag cgcttcctca ccctcggccc
      481 tgtccaccgc ggtgtaggcg gcggcatgga tcaccacatc gggcccgctg ccgccaaagc
      541 tggcggcgat ggccccggcg atgcccgccg cctcggccag atccagcgcc gggcggccca
      601 gcgcttccac ctgcacgcca gcgccagcgc gttccagcag gctgcgcacc acctggcctt
      661 cgcggcccgt aaccagcagc ttcacgcctg tttctccgcc accagcccca gccgcgcccc
      721 atcatagcga tcgcgcagcg gctgccacca ccagccatta tccagatacc agcgcacggt
      781 cttctcgatg ccgctgtcga aattctcctg cgcgcgccag cccagttccg tctccagccg
      841 ggtggcatcg atggcatagc gggcatcgtg gccaggccgg ttggtcacaa agcggatcag
      901 cgcctcgcgc ggggcagcgg ccggcgccag cgcgtccagc acggcgcaga tgcggcgcac
      961 cacatcgata ttgcgccgct cgttgcgccc gcccacatta taggtctcgc ccacccggcc
     1021 ctgccgcgcg atgagatcca gcgcgcgggc atggtcttcc acatagagcc agtcgcggat
     1081 gttctcgccg gtgccgtaaa ccggcagctc gcgcccggcc agcgcgttca ggatggtgag
     1141 cgggatgagc ttttcgggaa agtggtaagg cccgtaattg ttcgagcaat tgctcaccac
     1201 caccggcagg ccataggtgc gcgcccaggc cttggcgaga tggtcgctgg ccgccttgct
     1261 ggccgaatag gggctggacg gatcgtaggg cgtgtcctcg cggaacaggc cctcggcgcc
     1321 caggctgcca taaacctcgt cggtcgacac atggaggaag cggaaagcgg cctgctgcgc
     1381 tgccggcagc cccgcccaat aggcccgcgc cgcctccagc agggtgaagg tgcccaccac
     1441 attggtgtgg atgaaatccg ccgccccggt gatcgagcga tcgacatggc tttcagccgc
     1501 cagatggagg atgcgcgtgg gggcaaagga agcgatcgcc gcggccatcg ccgcgccatc
     1561 gcggatatcc gccttgagga agcgatggtt ggcacagccc tccaccgcct gcagcgaggc
     1621 caggttgccg gcataggtga gggcatcgat cgtcagcacc tcctcctgct gctccagcac
     1681 cagatggcgc accagcgcgg aaccgatgaa gcccgctccg ccggtcacga tcgtcctcat
     1741 gcgccggctc ctgccagggg gaacagcgcc ggcagatcag ccagcagcgg ctgcaccctg
     1801 tccttggccg acagcgtttc ggcatcggcc acatcgggcc aggcaatggc gatggccgga
     1861 tcgttccacg ccagcccctt gtcatgctcc gggctgtagt aggcggtcac cttgtagcac
     1921 accaccgtat cgggcagcag ggtgcagaag ccatgggcaa agccttccgg cacccacagc
     1981 tggtggccat tgtcggccga aagcgtggcg gccacccatt ggccgaaggt gggcgaacca
     2041 tggcggatat ccaccgccac atcgaggatg gcgcctgcca cgcagcgcac cagcttgccc
     2101 tggccggccg gcgctgtctg gaaatgaagg ccgcggatgg tgccggcctt cgcgctcagg
     2161 ctctggttct cctgcacaaa acccacatcg ccggccgccg cgcggaaggc gtcttcccga
     2221 aagatctcgg aaaaatagcc ccgggcatcg ccatggcggc gcggcacgat ctcgatgacg
     2281 ccggcaatcg gaaaagtcct gaattccatc ttggctgctg ctcgttcctg ccgtttgtgg
     2341 ccccttgcgg gccggcccgc gttctggacg aaggtgcccg cccgcacaag gcgatttgcg
     2401 cggccgggcc agcgctggcc atcgcctata tcgccatatc ggagatgctc tgtctgggcg
     2461 cggggcgccg gcagcggcac actgcgggca tggtggcagc acagcgaaag gccttcttcc
     2521 ccatgcccgc cgaacctgcc cggcgtttcc tcttcctgca gggcccgccc ggccccttct
     2581 tccgcctgct gggcgcaaag ctggccgccg ccggcgccac ctgcctgcgc ctcaacctca
     2641 acggggggga cgcgcatgac tggccgcgcc tggccgatgg ccgccgcacg cccgcctggc
     2701 gcggcccggc ccaccgctgg ccgcccttta tcgaggcttt cctgcgcgat catgccatca
     2761 ccgatctggt gctttttggc gattgccgcc cgctgcacat ggtcgcccgg cagatggcac
     2821 agctggccgg catccgcgtg catgtcttcg aggaaggcta tatccgcccc aactggctga
     2881 cgctggagca aggcggggtg aacggccatt cgccactgct gcgcgatgcc gccgcgctgc
     2941 tggccgccgg cacctgcctg cccccgccgc cagacctgcc gccgatcggc gcccacatgg
     3001 gccgccgcgt ggccgacagc tggggctatt tcggccatat gctgctgcgc gcgccgctct
     3061 atccgtttca ccgctcgcac cgcccgggct cgattgtgct ggaggggctg ggctgggccc
     3121 tcaaatatct gcgccgcgcc gcccgccagc ggcagacgga acgggcgctg gcggggctgg
     3181 ccgggcggcc ctatttcctg ttcccgctgc aattgtcggc agactatcag atccgcgccc
     3241 attcgccctt tgcccatatg gcccaggcgg cagaccatgt gctggccagc tttgccgccc
     3301 atgccccggc tgatgccctg ctggtggtga aggaccatcc gctggatgca acgctggccg
     3361 gctggggcgg cttcctcgcc cggcgggcgc gggcgctggg gctggcggcg cggatcatcc
     3421 atgtgccggg gggcgatctg gcggcgctgg cgctgatggc ccgcggcgtg gtggtggtga
     3481 acagcaccgc ggccactttc gccctggccg aaggggtggc ggtgaaggcg ctgggcacgg
     3541 cgctgtatga tctgcccggc atcacccatc agggcccgct ggccagcttc tgggccgcgc
     3601 cgcaagcgcc cgatccacag ctttatggca gcttctgccg ggtgctgcac cgccattgcc
     3661 tggtgcgcgg cgggctggcc agccgatcgg ccatcgccat tctggtgcaa aacgccgccg
     3721 cccgcctgct ggcgcccacc ctcgcgcaaa ccgcacagcc gcgcagcccc atccccgccg
     3781 cggcatgagc agcgggccgc ctccgggtca gatcgccgtc aacagggcgg ccaggccaaa
     3841 aaagatgccc ggcagattgg ccgccacgat cggccattcc cgccttttgc gcaggaagcc
     3901 ataaaggctc cacaagctgc aattggccac agctgccagc ggttggatga tcgatccctt
     3961 gtggccatcg agattaaggc ggatctgatc gatataggcc agatacatgg ccacagccat
     4021 gatcgacgca gcccagccga gaagcggcaa aaagcgctcg ttcacaatga ttttcccgtt
     4081 cacactccat gctgcaccgc agcataaagg tggggtatgc ggatcgcccg ggcctgtcca
     4141 cccgccgcgc ctcgcttcat cctgcccgat caacgcctca tcgtggcggc gagcctgcgc
     4201 ctagcgcagc gcccctgtcg tcaccaaccc gctttccacc gccacgatcg gcgtgaacag
     4261 ctggctgacc agctggatca gcttggtcgg ctggttggcc gcggcattgc cgatgaacag
     4321 cagatccttg tcgcgcatgg caaagcgctg cgagaggaaa taggccccgc cgcgcatcat
     4381 atccaggtga tagaccaccg gcttctcgcc gcccgcgcca tctgccacaa agcggaagac
     4441 gaacaccgcc ttggcatcgc ccagcgccgg atgggcgccg ccggccatcg ccaccgcttc
     4501 ggccagagag acgccggggc cggaaaaggc catctgctcc accttgccgg ccgcccccat
     4561 caccgcaaag ctgaacggct ggcgcagcac ctcgatgcga tcgcccggtg ccacgcgcat
     4621 gtcgcgcgcg ggcgcgctca gcacatcggc caggcgatat tccagcgccc ggccggcccg
     4681 caccacgcgc accgccagat ccttcgcctc gccgcgatag ccgccggcca gcgccaccgc
     4741 atcgctcagc gtctcgcggt tggtggccag caccagccgg cccggccggc ccacctcgcc
     4801 ccccacgatc acactgttgg caatcgtctg gctgatcgcc accaccacct gcgggttttg
     4861 cgacagaccc ttcagcccgg cgcggatctg cagcgccaat tcccccggcg tgcggccggc
     4921 cgcgcggatc ggcccggcat agggcaggcg cacgaggccg gcatcatcca cccggatgcc
     4981 gcccagccgc tctgcctgcg cgccgccggt caaccccgcg ccctgcgcat cgccgccgcg
     5041 cgcgccggca aacagggtga cgcccgcttc atagatggcg atatccagca catcgcccgg
     5101 gccgatgaga tcggtgggca gtggcgcccc ggcgggctgt tccaccggcg gggcgggcgg
     5161 cgccggcggc aggctggcaa aattcgccag ctccaccacc tgaaagggca ggccggcggc
     5221 cgcgccctgc cccgggccgc ccccagcctc tcgcagcacc tcatgcccgg tgggccccga
     5281 taccggcagc gcggcacaac cgcccgtgcc cagcaacagc gccagcaccg ccacgcgctg
     5341 cccgccacac ccctttgccg cttgcgcaaa ccccatggaa aaccccgctg ccccagccaa
     5401 aatcctctcc cccctgtcta gcagcggcag cggcgggcca gccaatgcag gcgcatccat
     5461 ttaggttctt ctacggaaag ggcgatcctg caggcgcctc caaaaccgag ccactccggg
     5521 ctaggcgggg tggcgccggc cgctagtcta ccggcgcata tggctctcat tcctccctcg
     5581 cgccttgcgg cgatcccgca tctgggcacc ttccttgccg ggcatgagcg ggcggtggtg
     5641 ggctgggggc gcaagccttc gggccggcgc gcgcaaaggc tggcgggctg gctggggcgg
     5701 ccggtcgcgc tgctggaaga tgggctgctc cgctctgtcg ggcggggcga tccgccgctc
     5761 tcgctgctgg tcgatgatat cggcgtttat tacgatgccg cggccccttc gcggatggag
     5821 cacagcattg ccgccggcgc ctcgccccgg caggcggcgc gcgcgctggc gctggcggcg
     5881 gcgtggcggg caggcggcct ttccaaatac aaccacgcgc ccgatccggc ggcgctgccc
     5941 acaggcccct atgtgctgct ggcagaccag tgccggggcg atctgtcgat tgccggggcg
     6001 caggcctgcg ctgccagctt tgcccgcatg gcagaggccg ccgcggtgga atttccccat
     6061 cacgcgctgg tgctgaaaac ccatcccgat gcccacagcc atggccgcgc cagctgcctg
     6121 ccgccggcgc tgggcgccca tccgcgcgcc gtgccggtgg ccgatggctg ccacccggcg
     6181 cggctgatcg cgggggcagc ggcggtctac accgtcagct cgcaaatcgg gttcgaggcg
     6241 ttgatctggg gccggccgct gcgctgcttc ggcctgccct tctatgccgg ctggggcctg
     6301 gccgaggagg accgcccgcc gcccgcccgc cgcgccgccg gggcccggct ggaagatctg
     6361 ctgcatgccg cgctggtggt gctggcgcgc tatgtcgatc cggccagcgg gcgggcgtgg
     6421 caggcggagg aggctttcgc ccatgtcgcc agcgcccgcc ggcagcgcgc ccggccagcg
     6481 ggatagggca tggccggccg gcccaccacc gacctttatt tccgcaacgc gctggcgccc
     6541 ttcatcgcgc cgcgctggtg gccagtgggg tggccggcgg ggcaggcagc gcccatccgc
     6601 gcctgcgggg cgcattcctt cagcgccggg cgctggcggg tgctgctgcg cgaggatgcg
     6661 ccggcagcga tgcgcgccgc gctcgatcat gcagggccgc tggcctatct ggtggacgat
     6721 gattttgccg ccgcggccag ctgccccgcc ctgcccgagg cctatcgcca gcgcctggcc
     6781 gcctttgccc ggggctggca cgccgcgctg ctggcgcggg ccgatgtggt gctggccgcc
     6841 tcggcgccgc tggcagcgcg gctggccggc cagcccggcc ttgccccccg gctgcggctg
     6901 atagaaccgt tctggcggga aagcccggcc gatcaggcgc attttgcccc gctgacaggg
     6961 ggcgcgccct tgcgcatcgc ccatctgggc agcgccagcc atggcgccgc gctggccgcg
     7021 ctggccccgg ccctggccgc cctgctggat cgccacccgg cctgccagtt cacctatttt
     7081 gcccgcgcgc cggtggatcc ggcgctggaa gcccaccccc gcgcccgccg gcagcagccg
     7141 atggcctggc ccgaataccg ccgctggctg gcccggcagc gctttcacct cgccctttat
     7201 ccgttgctgg acaatgggtt cgacagggcg cgctcggcca gcaagctggc ggaacatgcg
     7261 gtggcgggcg cggtgggggt ctatcccgcc ggctgggcgc cgggggcggc gctggcggcg
     7321 gccggcggcg cgctgctggc gcccgacagg gtggaactat ggcccgccgc gatggaccgg
     7381 ctgatcggcc agatcatgca aaaacaccac aagctggcgc aaatcgcggc gcaggcggca
     7441 aagacgttgt cgatcaacga cccctgtgcg aaacaaaggc aaatatggag caacagcctg
     7501 gatctttgcc tcgcgtgaat ttcacttcgg cctttgtgcg cacggtgttc tggcggcccg
     7561 gccgggctct ttcggcgctt tactggcaat tgaccggccg gcgcaaacgc gcgcgcaacc
     7621 ggctgcgact cgaaatggcg caaagcccgc gcgcctatgc ccgctggatc gcctcggtgg
     7681 agcggcagga ggatgtgctg gccgccgccc ccgacaggat cgcgcaatgg ccaacccgcc
     7741 cgctgatctc tgtggtgctg catctggaag cgggcagccg gcaggggcag gtggaacgct
     7801 gcctggcggc gctgcgcgcc cagtgctatc ccgaatggga actggtgctg gtgcaatcct
     7861 atgatgcccc gcccacccgc agcatcgatg tgccgcgcct gctgctggcg cccagccgcg
     7921 ccggcagccg cgcccaggcg ctggcgctgg gggcggaatg cgcgcgcggc agccatatcc
     7981 tgccgatccg cgccgaacat gtgctggcgc ccaccgccct gctgcatctg gccaccgcgc
     8041 tgcaggcgca aagccctgcg ccccacctcc tctatggcga ccatgaccgg gtggaccgcg
     8101 ccggccgccg ccacagcccc tggttcaagc cggaatggaa cgcggaactg gcgctggggc
     8161 aggattatgt ctcgcaggcc tgcctcattc gccgcgatgc cgcgcgcgcc tgcctgccca
     8221 ttccgcccgc gctggccgat gccgccacct atgcgctggt gctggcggtg agcgcgctgg
     8281 ccgaggcaca gatcgtgcat gtgccgcaca tgctgtgcca ctgcaccagc ggcgaaagcg
     8341 tgaacgagcc ggccgcccgc gccgccgtgg tggaacggca cttggccggc agcggcgcca
     8401 gcctgcggct gggcagccat ggcacggtgc aggtgcactg gccgctgccc gatccggcgc
     8461 cgctggtcag cattctggtg cccacgcggg acaggctgga tctgctgcgc gcctgcatca
     8521 ccggcgtgct caacgccacg cgctatcccg ccttcgaagt gctgatcatc gacaatggca
     8581 gccaggatcc ggccacgctc gattatctgg cgcgcatcgc cggcaatccg cgcgtgcgcg
     8641 tactgccctg gcaccggccg ttcaactatt ccgccatcaa caattttgcc gcgcgcgagg
     8701 cccgcgggcg ctatctgtgc ctgctcaaca atgatgtcga tgtggtagac gatcagtggc
     8761 tgccggcgct gatgcggcag gccgcgcggc cccatgtggg ggcggtgggc gccaagctgc
     8821 tctatgccga tggccgcatc cagcatgccg gcgtggtggt ggggctgggc gatgcggccg
     8881 gccatgccca ccgtttccag cgcgacagcg attgcggcta tttcgcccgg ccccatgtgg
     8941 cccactatgt cagcgccgtc accggggcct gcctgctggt ggagaaggcc aagttcgagg
     9001 cggtgggcgg gctggacgag gaggccttcc cggtcgcctt caacgatgtc gacctgtgcc
     9061 tcaagctggc agcggccggt tatcgcaatg tctatgtgcc gcaagcggtg ctggtgcatc
     9121 atgaatccgc cagccgcggg cacgacagcc tggccgccaa ccgcgcccgc tatgccggcg
     9181 agctagccgc cctgcgcgcc cgctggcaca ccgaaagctt cctcgacccg ctgcaccacc
     9241 ccgagctgga acgcagcagc gagacctatc tgatccgcca gcccgcctaa agcgcctcgt
     9301 aagcggccag cccctcttcc acatcggcaa acagccggcc gtgcccgcct tccagcagca
     9361 gcgcgctatc gcacaggcgg cggatcatct cgggcgcgtg gctggccagg atcagcgccc
     9421 ggtcggcccg tttctcgaac agttccgcca tgcatttgcg ctggaaattc tggtcgccca
     9481 ccatgataac ctcgtcgatc agatagcagt cgaactcgat cgccagcgac agggcaaagg
     9541 ccagccgcgc cttcatgccg gccgaatagg tcttcaccgg catcgccagc tgccggccca
     9601 gctcgctgaa atcctccaca aagccgcgga tatcggcata atcgcggcca tagatgcggg
     9661 cgatgaagcg ggcattgtca aaccctgtca ggctgccctg aaagccgccg ccaaagccca
     9721 gcggccagga caggctcatc tgccgctcca ccacgccgct gctgggcagc tccaccccgc
     9781 cgatcagctt gatcagcgtg gtcttgcccg cgccattgcg gcccagaatg cccagccgct
     9841 ggcccgggcg caccaccaga tcaagcccgg ccagcacctg tttgcgggcg cgcccttgcg
     9901 ggtaatgctt ggcaaggccg gtgcagcgga tcatgcttcc accaccagat gacggcgcac
     9961 ctgccggcac agccacaagc ccaccgccat gatcgcgcct gacaccgcca gcggatagaa
    10021 cagcgcgtaa taggcctgat gggccgggcc aaagaggccg gcgcgcatca tttccatccc
    10081 gtgcaccggg ggcgaacaca gcagaagagc ccgcgccccc ggcgccagcc aggccgccat
    10141 ggtgaatgcc cccgaaaacg gcaccatcag ataaacgctg accggcataa ccttctccag
    10201 caccggcgac agctcgctga gcggcgccag caggcagcac atcgccagcg tgaaccagcc
    10261 atagaccgcc cagcccgcca gcatcagcgc cagatcataa ggcatgggaa acaggcccag
    10321 cgccaccatc accacgccaa tgaacagata ggccatcatg tggccgatgc attccacgca
    10381 aaagcgcacc agcacgaaat cctggatgag gatctggcgg tgatacatca ggctggcatt
    10441 ggcctgaaac agccccaccg accggttcac cgcattgcgg aacagcacca tcggcagata
    10501 gccggtggcc acgaaagcga agacgttgat gccatgctcg ctcaccccat gcatgatgcg
    10561 ccacaacagg cccaccagcg tggcaaacag cagcggctct gccatcaccc agaggaagcc
    10621 gatgttctcg cgcccgaagc gggtggtcag ctcgcggatc atcagcgcgg cgatcacccg
    10681 ccgctggatg cgccagccag ccgccagccg ggcgccagtg tgcgattctg tgtgcgatac
    10741 cgccatccct cactcctccg gcgcgtgttc gagcaggccg accaggaaca tccagccgat
    10801 cgcataaagg cacaggctgc tggccagcac cgtcagcacg atgcgccagc gatggggcag
    10861 cgcgggccag tccggccggt tgggctccac cacgcgctca aggtagaatt gctggcgggc
    10921 ggcatcgctg cgggcctgtt ccaacgcggt gttggcggcg gccagcgcct gggtggcaaa
    10981 ttcctgctcc agcgccagcc tgtcatagcc ggcaagcttg gccgcaatcg ccccgccgcc
    11041 gcccaccgcg cggccctcct gcaggccggc ctgggcggcc atggcggcca cccgggcgcg
    11101 caaggcgggg atcgccggat tggcaggggc agccttttcc atcagctgca actgggcggc
    11161 cagcgctgcc tgctcggcct ccagccggtt gctcacatcg agcgcgccgc cggcctgcct
    11221 ggccggatcg atcagcgcct gggcattgcg ataggccccc agcgccagcc gcgcgcgcgc
    11281 cagcctttgc tcggccagcg ccacgcggcg ctcgctctcg gcgatggcgc gcgcctcggc
    11341 ccgctggttc agccggttga ccagcccctc gcccgcgccc agcagggcca ggttgatcgc
    11401 ctgcgcatcc ccggcgcgat aggcccgcac ctccagcacg gcaatgccgc tttccggatc
    11461 gatccgcgcg ctcaccttgc cggtgtagta gcgatagagg ccctcgaacg tatcggcctg
    11521 aaacgggccg ggatagcgcc ccagccaatc gcccccatgg ccataggcag cgcgcagatc
    11581 cgccttgccg cccagttccg ccagcgcgct gcgcgagcgc aggaaatcga ccacttcgct
    11641 ggcctgttca tgggcggtgc cgggcgtcgt cgtctgcacc agattggcca gcgttgcgat
    11701 ctgcgcgctt ttctgccccg ccccacgaat gacaaagcgc gcctgcgaga catattcatc
    11761 cgccgcaaag ccgccgaaat agatcgcgct gagcagcgtg ggcacgccca ccaccagcgc
    11821 catccagcgg tgccgccaca gcccgcccgg ccaagcccgc cggcgcccgg ccgccacggc
    11881 cgcagggccg gcaccgccat ctgctccatc ggccggtgtg aacatccgcc gcatttcctg
    11941 cacaaccatc tgtgtcatcc cctgccccct gttggtgccg gcgcgccggc cctcatctcg
    12001 tcgatcagcg cggccagcac cgccccattg ccggcctgca gggcgcgcgc atgggcggcg
    12061 atcgcgcccg catccacgcc ccctgccgca tggcgccgca tggccccggc cagcgcccgc
    12121 gccaggccgg cctcgtcggc aaaggtccag ccatcggcaa ggccggccac ttgcgccgcc
    12181 ggcccgcccc tggcgtgcac caccggcgca cagccggcca gcatcgcctc atagggggca
    12241 atgccgaaat gctcctgctt ttcaggcgcg gcatcgggca tgccaaggcc ggtggcgtgg
    12301 caatagatcg ccgatgccgc acaggcctgc gccacttgcg cccgcgtggc attggcgatg
    12361 atctgcaccg gcgcgccggc cgccagcgcc tgcagatcgg ccagatagcg cgccgcgctg
    12421 ggctggctcg cctcgaaact gccaatcacc accagttccc aatcggccag atcccggtgc
    12481 cggcgccgca catccagaaa ggcgcgcagg atctggtggt ggttcttgca atggccatca
    12541 taggtgaagc ggcccagcgt gaggatctgc cgccgcttgg catccgcgcg cgcgccgccc
    12601 ccggcaaacc ccaggatcgg cagcagcacg cggccatcat gcgcgcccca cagccgcccg
    12661 gcccaatcgc gcgtgtaggg cgaattgtga aggaaagtgt aggccggcag gatcgccgga
    12721 tcgacatcgc gatgcggaaa ggagacgaca tagatattgc gccccgccgc cggcaccaga
    12781 ttggaacgga aggtggcatt gatgaagaca tcgaatttcg cggtgaaatg cgtgtccact
    12841 tcggtcgaca ccagcttgcg cacatgggca aggtccaccc cgaaataggc ttccagcccg
    12901 gccatgtcga aatcgctctc gctggcaaga tagacctcgc ccagccccgc cagcgcgcag
    12961 gcgatatcga tcgcatgttt ctcgcccccg cccatgctgg cgaaatagct gttgtagatg
    13021 cagatcttca gccgccccgg ggccaccagc gccgccccgc cgcctgccgc ccgcacatca
    13081 tcgtcatagg cttccagccg ctggcgcagg gcggggggca ggcccgcctc ataatggggc
    13141 gcgaaatcgg ccagcggtgc gccccctgtc aaccggcgat agagatcgcc gctgcgcgcc
    13201 accagcgtgc gccacacagg gctgtgctcc tcggtcgatt ccgaatggcg atgggccacg
    13261 cgcgcggccg gctgataaag aatgcgataa ccccgcccgc gcagccaggc cgacagttcg
    13321 ctatcctcga aataggcgaa gaaggcaggg gcaaacagct tgcgctccat caccgccacc
    13381 cgccgcacca gcgccatgca gccacacagc gcctccacat aggccttgct gaagaagcgc
    13441 ccgtcatcct cctcgccaaa gccgcggtca taaggggtgc catccgcgcg caggccgctg
    13501 ccggcattgt tcaccagcgg ccgcgcgctg gccgcatggc gcgcctcata aggcaggcag
    13561 gccaccaccc gccctgctgg cgctgccacg aaactggcca ccggcgcatg gcccagcccc
    13621 acccttgcct gcctgagccc gcgcccgccg gccagttcca gccgaagcgc gcgcctggca
    13681 tggggcaggt ccacctccag ccggccatcg ccatcgctgt ggatctgcgc cccctcctgc
    13741 ctgccattgc gcacgaacag cttggcataa tcgagccccg ccagcagatc cgcccgggcc
    13801 aagctgaagg gcgcatccgc ttccaccgac aggcgcagga aaggcgtgaa gaaatcgatt
    13861 ttgggcacgg ccacggccgc gctggcatcc atccgcagcg cctccaccag ctgttgcagg
    13921 caatcgggcg ccaacaccgt gtccgggttc accagcgcga tgaattcccc ccgcgccagt
    13981 tccaccgcaa tattgttcgc ctcggcaaag ccggggttat ccgcccgcag ccagcggcaa
    14041 tcggggatat gggcgcggca cagggcttct gtctcctcca tgccgttttc caccacgatc
    14101 acttccagcg gcgcccagct ttgcgccgcc agcgtgggca gcagcgcggc cagatccttg
    14161 ccggaattga agctgacgat aaccaccgag atcagcgggc aatggcggat gatgccaaag
    14221 gagcggaacg gcacttcggc ccgccccgcc gccggccgcc tggccagcgc cgcctgcggc
    14281 cccgccggcc ggccgatgcg cgcgccgatc agccggtaat gctctgccgg ggccaggcca
    14341 gacaggccga catcgggaaa gcgcgccgca taccatgccg catcgaagcc cgccggcaca
    14401 tccgccgccc tgtcgatcac gctcatgccg cgcccccttc atggcgcccg ggcacgcagc
    14461 gcccctcggc cagcccatgc tgcaacagat ggcgcagcgg ctccgccccg gcggcgcgca
    14521 catcaggatg ggcggccaga taggccgcgg catcgaacag gcccgcgccg gccagccgct
    14581 ggtgctggcg ccgcaagcgc aagggcggcg gcagccaccc ctcccacccg gcccggcacg
    14641 tgagcaccgc ccccagcccg cgcagccagt tggcctcctc ggcccgtgcg gcagcggcgg
    14701 cctctgcctg ctgcaacagg cgcgccatcg cggccatctc gtggaaacgc gcggccagat
    14761 tttcttccag ctcggccagc cgggcccggg cggcctgcgc ggcttcctct gctgcgcggg
    14821 tggcatcggc cagcagggcg gcctgcgcgg cctctgccgc ctgtgccgcc tgggcctgct
    14881 gtgccgcctg cactaccgcc tgggcctgct gctgggccag cgcttgctgc gctgcatcgc
    14941 gcgcgctggc cagagcggcc tgcccggcca gcgcggcctc cagcgcctgc gcctgctgcg
    15001 ccgcctgccc gcgcgcttca gccagcgccg cctgcaaggg cgccacctcg gccgccgccg
    15061 cccgtgcccg tgccagcccc cgcaaggcgc ccgccagttc ctccttcgcg cgcagcaggg
    15121 tgccaaaccg ctgctgctcg cgcgcttcgg cagcggccag cgcccggcgc gcggtgttca
    15181 actgcgcggc ctgcccggcc agcgccgagg cagccgccgc cgcttcggcg cggctatcgc
    15241 gcagggcgct ctcggcctcg gccaattgcc cggccagcac ttcggcatcg cgcccacgtt
    15301 ctgccaaggc cacttcggca gcggccagcc gcagggcgat ggcgccttca cgcgcctgcg
    15361 cctcggcctg cgctctggca agatcggcgg caagatcggc ggcgagttcg gccgcaaggg
    15421 cggtgcgcgc gcgcgcctcc tcctctgccg cgcgggcatg gagcgcccgg tccgccgcca
    15481 gttcgcccgc cagcgcgccg gcccgcagca gccccgcctg cccccgcgca accaggccgg
    15541 caaacccttc ccccgcctca tcgaaagcca cgcggatcgc atccagccgg gcataatcgg
    15601 ccgctgcctc ctcccccgcc gcccagcgca gcaggatggc ataagcctcg cgcacccagc
    15661 cgcagatcag cggattatcc agcacctggc cggccggcac ctgatggtgg cgcagatcgg
    15721 ccgccagaaa cgcgccaatc tcctcagccg tgctggccga caggcgcggc cagcccagcc
    15781 ccagatccac ccccgcccgc
//
LOCUS       DASRYE010000096         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_38578,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000096 DASRYE010000000
VERSION     DASRYE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38578"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFF98_05425"
     CDS             complement(<1..331)
                     /locus_tag="VFF98_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63103.1"
                     /translation="MAVSTFLWPLLQDGQLIERELLLFALFWFAIGLFDELLIDFIWL
                     ILQVNSKKPARAASPAGLSGRLAGPMAVLIPAWQEAAVIGATISHMLRAWPQGDYRLF
                     IGCYGNDP"
     gene            complement(529..957)
                     /locus_tag="VFF98_05430"
     CDS             complement(529..957)
                     /locus_tag="VFF98_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU63104.1"
                     /translation="MSRFLHSMIRVSDPDQTIRFFALLGLAETRRFSSEQGRFTLIYL
                     ACPGETAEVELTYNWPAEDGSAEAYAGGRNFGHLAFQVEDIYATCQTLADAGITINRP
                     PRDGHMAFVKTPDGISIELLQNGHLEPKEPWLSMPNSGIW"
     gene            1241..2050
                     /locus_tag="VFF98_05435"
     CDS             1241..2050
                     /locus_tag="VFF98_05435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZU63105.1"
                     /translation="MIASLRRLLRLDHARQLPALPPGQRAYAIGDIHGRADLLARLID
                     GIEADDAARNASTPCQTTMILLGDLIDRGPDSAGVIALARAWQQRRPVRILAGNHEEM
                     LLEGLERLEVLRHLLQHGGRETALSFGVDAQAYLKATWEEAQALLRAAVPADVLDFLR
                     SFEDHVQLGDYLFVHAGIRPGVPIEAQTPADLRWIREPFLSCTDSHGAVIVHGHTITD
                     EPALRPNRIGIDTGAFQSGRLTALGLEGTRRWLIEARETDGVISLSHTGLN"
     gene            2089..>3291
                     /locus_tag="VFF98_05440"
     CDS             2089..>3291
                     /locus_tag="VFF98_05440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZU63106.1"
                     /translation="MKIAMVGSGYVGLVSGACFADFGHDVVCIDKDPGKIERLNAGIM
                     PIYEPGLAELVAGNVKAGRLSFTTNLAEGIEGAAAIFIAVGTPSRRGDGHADLSFVYA
                     VAEEVGRNLKTPAVIVTKSTVPVGTGDEVERIIADTGTGVRFAVASNPEFLREGAAIG
                     DFKRPDRIVIGTEDEWAKGVMNEVYRPLFLNKAPILFTARRSSELIKYAANAFLAVKI
                     TFINEMADLCEKVGGNVQDVARGIGMDNRIGSKFLHAGPGYGGSCFPKDTLALLKTAE
                     DYDSPTRIVEAVVKVNDSRKRAMGRKVIEALGGQGEARGKRVALLGLTFKPNTDDMRD
                     APSIAIAQALSDAGVDIAAYDPEGMEQAQPLMPGVAMKENAYAAIEGADAVVIVTEWD
                     AFRALDFER"
BASE COUNT          656 a         1065 c         1025 g          545 t
ORIGIN      
        1 ccggatcatt gccatagcag ccgatgaaca gccgataatc cccctgcggc caggcccgca
       61 gcatatggct gatggtggcg ccgataacgg ccgcttcctg ccaggcggga atcagcaccg
      121 ccattggccc tgcgagtcgg cccgaaagcc ccgcaggtga cgccgcgcgc gcgggctttt
      181 ttgaattaac ttggagaatc agccatataa aatcgatgag caattcatcg aagaggccaa
      241 tcgcaaacca gaaaagcgca aacaggagca attcccgctc aatcagctgt ccatcctgca
      301 acaatggcca gaggaatgtt gaaacagcca tgtctgtcgc ccccacctat ccccccgcat
      361 aggtagatat acttattatt gttcctcgtc cctagcaaca tccttgaaaa gtggattgac
      421 gcatatcaac cgtaggaaaa tgacagatgt gtcagcagta aacatcctgt tcgcagccgc
      481 acaaaatggc ggggagcggc acaacatgcc attccccgcc caatacagtt accagatgcc
      541 gctattgggc atagacagcc acggctcctt cggctcaaga tggccattct gaagcaattc
      601 gatagaaata ccgtccggcg tcttcacgaa ggccatgtgg ccatcgcgcg gggggcggtt
      661 gatcgtgatg ccggcatcgg ccagcgtctg acaggtggca tagatatcct ccacctggaa
      721 ggccagatgg ccgaaattgc gcccgccggc ataggcctcg gcgctgccat cttcggccgg
      781 ccagttatag gtcagctcca cttcggccgt ctcgcccggg caggccaggt agatcagcgt
      841 gaagcggccc tgctcgctgg aaaagcggcg ggtttcggca aggcccagca atgcgaagaa
      901 gcggatcgtc tgatccgggt cggaaacgcg gatcatgctg tgcagaaaac ggctcatcgg
      961 tatatccttg ttgcgggcga acacaataaa acgcggccgc ccatcgggca caacccatca
     1021 ggcacaaaca acccatcagg cacaggcggc gccggctttc atagaaaccc gcaggccccg
     1081 gcgccagttc ccgccgcgcc ggcattctcg cccgcggccc gcatcgtgtt gcaatgcggc
     1141 aagatattcg caagcgcagc caatattcgc aagcgcacct tgacgcccgc aaagacaagg
     1201 gcataaggca tgtcgtagtt gcccctatgg ccttaaaccg atgatcgcat ccctgcgccg
     1261 tctgctgcgc cttgaccacg ccaggcaact gcccgcgctg ccgccgggcc agcgggccta
     1321 tgccatcggc gatatccacg gccgggcgga tctgcttgcc cggctgatcg acgggatcga
     1381 ggccgacgat gccgcgcgca acgccagcac gccctgccag acaacgatga tcctgctggg
     1441 cgacctgatc gaccgcggcc ccgacagcgc cggcgtgatc gccctggccc gggcctggca
     1501 gcagcgccgg ccggtgcgca ttctggccgg caaccacgag gaaatgctgc tggaagggct
     1561 ggagcggctg gaagtgctgc gccacctgct gcaacatggc ggccgcgaga cggcactgtc
     1621 tttcggggtg gatgcccagg cctatctgaa agccacctgg gaagaggcgc aggccctgct
     1681 gcgcgccgcc gtgccggccg atgtgctgga tttcctgcgc agttttgaag atcatgtgca
     1741 gctgggcgac tatctgttcg tgcatgccgg catccgcccg ggcgtgccga tcgaggcgca
     1801 gacgccagcc gacctgcgct ggatccgcga gccgttcctc tcctgcacgg acagtcatgg
     1861 cgctgtcatc gtgcatggcc acacgatcac cgacgaacct gcccttcggc ccaatcgcat
     1921 cggcatcgac accggcgcct tccaatccgg caggctgaca gcgcttggcc tggaaggcac
     1981 ccggcgctgg ctgatcgagg cccgcgagac agacggggtg atctccctct cccacaccgg
     2041 gctcaactga acagcgcccg tatcacagct taacgcaagg agccggtaat gaagatagcg
     2101 atggtcggat cgggctatgt cggactggtg tctggcgcct gctttgccga ctttggccac
     2161 gatgtggtgt gcatcgacaa ggaccccggc aagatcgagc ggctgaatgc cggcatcatg
     2221 ccgatctacg agccgggcct ggccgaactg gtggctggca atgtgaaggc ggggcgcctc
     2281 tccttcacca ccaacctggc cgaggggatc gagggggccg cggccatctt catcgcggtg
     2341 ggcaccccca gccgccgcgg cgatggccat gccgatctct ccttcgtcta tgccgtggcc
     2401 gaggaagtgg gccgcaacct gaaaacgccg gcggtcatcg tcaccaaatc caccgtgccg
     2461 gtgggcacgg gcgacgaggt ggagcggatc attgccgaca ctggcaccgg cgtgcgcttt
     2521 gcggttgcct ccaacccgga attcctgcgc gagggcgcgg ccatcggcga tttcaagcgg
     2581 cccgaccgca tcgtcatcgg caccgaggat gaatgggcca agggggtgat gaacgaggtc
     2641 tatcgcccgc tgttcctcaa caaggcgccg atcctgttca ccgcccgccg ttccagcgag
     2701 ctgatcaaat atgccgccaa cgccttcctc gcggtgaaga tcaccttcat caacgaaatg
     2761 gccgacctgt gcgagaaggt gggcggcaat gtgcaggatg tggcacgcgg catcggcatg
     2821 gataaccgca tcggttccaa gttcctgcat gccggccccg gctatggcgg cagctgcttc
     2881 cccaaggaca cgctggccct gctgaagacg gcggaagact acgacagccc cacgcgcatc
     2941 gtcgaggcgg tggtgaaggt gaacgacagc cgcaagcgcg ccatgggccg caaggtgatc
     3001 gaggcgctgg gcggccaggg cgaggcacgc ggcaagcgcg tggcgctgct gggcctgacc
     3061 ttcaagccca acaccgacga catgcgcgat gcgccctcca tcgccatcgc ccaggcgctg
     3121 agcgatgctg gcgtggacat cgccgcctac gatccggaag gcatggagca ggcccagccg
     3181 ctgatgccgg gcgtggccat gaaggaaaac gcctatgccg ccatcgaggg cgccgatgcg
     3241 gtggtgatcg tcaccgaatg ggatgccttc cgcgcgctgg atttcgagcg g
//
LOCUS       DASRYE010000097         4893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_129415,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000097 DASRYE010000000
VERSION     DASRYE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129415"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFF98_05445"
     CDS             complement(<1..267)
                     /locus_tag="VFF98_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HZU63107.1"
                     /translation="MEADEARDLIEETVEEAKAADAGERAFRARVSVLVGVFALLLAL
                     IHMAGAAAQRDSLLNAIEASDTFAYMQAKTVRETVLLTNANMPGL"
     gene            complement(402..1775)
                     /locus_tag="VFF98_05450"
     CDS             complement(402..1775)
                     /locus_tag="VFF98_05450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZU63108.1"
                     /translation="MAQNWTPDSWKTREARHLPVYNDAEELGRVEQTLAKFPPLVFAG
                     EARALKAELADVAAGKGFLLQGGDCAESFAEFHPDNIRDTFRVLLQMAVVLTFASKQP
                     VVKVGRMAGQFAKPRSAPTETLGGVELPSYFGDIINGADFTPESRRNDPARMLQAYSQ
                     SAATLNLLRAFASGGYANLRQVHKWTLEHIEKSPWGDRFAEMADRIGEALDFMAACGV
                     DPSSVPQLQSTHFYTSHEALLLPYEEALTRRDSLTGDWYDCSAHMLWIGDRTRFDNSA
                     HVEFLKGVGNPIGVKCGPSLEPDMLLRLLDELNPAREGGRITLIARYGHDKVEAGLPK
                     LVRAVQAEGHPVLWSCDPMHGNVVKSDSGYKTRPFERILAEVRGFFAVHRAEGSHAGG
                     IHIEMTGKNVTECTGGLIDITDEALADRYHTHCDPRLNGAQSIELAFLIAEELNKEAQ
                     DRVKAAA"
     gene            complement(1898..4834)
                     /locus_tag="VFF98_05455"
     CDS             complement(1898..4834)
                     /locus_tag="VFF98_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZU63109.1"
                     /translation="MRFPARMPLRLVSALAIVLALPLVPVTSHARAPAAAAQTGASAP
                     SALPPLPDQANPWLYVNSDVPRDKEWTFGKLANGLRWAVRANGVPPGQVSIRIRMDVG
                     SMFEKPEEAGFAHLIEHLVFRQSKYLGQAQAIPTWQRLGATFGTDTNAETSPTQTVFK
                     IDLPDATPASLDESMKLLSGMMIAPNLSESDIRTEVPIVLAEKRERGGQLERIADAMR
                     EVVGAGQPLGDHATIGSVAVLEAAHQEGVRAFHQRWYRPENAAIIISGDADPRLMAAL
                     IGKWFGDWQGVGPHVPVPSFGAPAAPAGADPANPVGETRVVVAPGQQRAITIATLRPW
                     HEKNDTIVYNQGQMIDQVALAIINRRLEARARAGASFLQAQVGQENESRSLDGTFTAI
                     TPIDGDWRAALKAVRAVIADALDTPPSKEEIAREAAEINVVYVSQVEQRRLLPGARVA
                     DDLVGALDIHETVTSPEGMLEVYQKSLPLFTPEAVLEHTRKLFAGAVTRAIYVTPDAA
                     EADAGALAAALRGKVAPDAHARPGSHAISFADLPPIGPAAPAPVSHPSGLLGIEELRF
                     ANGVSVQLWPAHDEPGRVGVRVRFGAGYRAFQPADAAYATLGTMALVGMGEGPLGQEE
                     LDSISTGRKMGFDFSIDDGNFQLSAETRAEDLADQLYLFAEKFAQPRWDAGPLARARA
                     AALQQYDSLASSPQGVLNRDLKALQRGGDWRFGVPSKAALAAVDVAGFKARWAPVLAQ
                     GPIEVQIFGDFDRDKAVAALQRSFGALAPRGDLPAATPTQVGTARPTPTPIVLRHTGD
                     ANQAAAAVSWSTGGGLAGIRESRQLEVLSQVFSNRLLDAMREKEGASYAPQVSSDWPL
                     DLANGGTMTALAQLQPDKVPDFFTTVDAIAADLAARPISADDLARVIEPLRQQIARAT
                     SGTAFFMGQVAGGTRDASRYAALRSLLADYTDVTPERLQQLAGQYLLPGRAWRLEVLP
                     EGAKTR"
BASE COUNT          782 a         1685 c         1623 g          803 t
ORIGIN      
        1 gagcccgggc atgttggcat tggtcagcag caccgtctcg cgcacggtct tggcctgcat
       61 ataggcaaag gtgtcagacg cctcgatggc gttcagcagg ctgtcgcgct gggcggcagc
      121 gccggccatg tggatcagcg ccagaagcag cgcaaacacc cccaccagca ccgacacgcg
      181 ggcgcgaaag gcgcgctctc cggcatcggc cgccttggcc tcttccacgg tttcctcgat
      241 cagatcgcgc gcttcatccg cttccatcgc ctgcccttcc ctcctgcctt ggccggcggg
      301 tgatactgcg ccgatgggca aaggcaatgg ccggaatagg aaaatgcgaa aagggggcag
      361 gcgcgcgcag cccctgcccc ccattcagcc acgcaagcgg atcaggcggc ggccttcacc
      421 cggtcttgcg cttccttgtt cagttcctcg gcgatcagga acgccagctc gatcgactgg
      481 gcgccattga gccgcggatc gcaatgggtg tgatagcggt ccgccagcgc ctcatcggta
      541 atgtcgatca ggccgccggt gcattcggtc acattcttgc cggtcatctc gatgtggatg
      601 ccgccggcat ggctgccctc ggcccggtgc acggcaaaga agccgcgcac ttcggccaga
      661 atgcgctcga aaggccgggt cttgtagccg ctgtcgctct tgaccacgtt gccgtgcatc
      721 ggatcgcagc tccacagcac cgggtggcct tcagcctgca ccgcgcgcac cagtttgggc
      781 aggcccgctt ccaccttgtc atgcccatag cgggcgatga gggtgatgcg gccgccctcg
      841 cgcgcggggt tcagctcgtc gagcaggcgc agcagcatgt ccggctccag gctcggcccg
      901 catttcacgc cgatggggtt gcccaccccc ttcaggaatt cgacatgggc cgaattgtcg
      961 aagcgggtgc gatcgccgat ccacagcatg tgggccgaac agtcatacca gtcgccggtc
     1021 agcgaatcgc ggcgggtcag cgcttcctca tagggcagca gcagcgcctc gtggctggtg
     1081 tagaaatggg tggactgcag ctgcggcacg ctcgacggat cgaccccgca ggccgccatg
     1141 aagtccagcg cttcgccgat gcggtcggcc atttcggcaa accggtcgcc ccacgggctc
     1201 ttctcgatat gctccagcgt ccacttgtgc acctggcgca gattggcata gccgccgctg
     1261 gcaaaggccc gcagcaggtt cagcgtcgcc gcgctctggc tataggcctg cagcatgcgg
     1321 gccggatcgt tgcgccggct ttcgggcgtg aaatccgcac cgttgatgat gtcgccgaaa
     1381 tagctgggca gctccacccc gcccagcgtt tcggtgggcg cgctgcgcgg cttggcgaac
     1441 tggccggcca tgcggcccac cttcaccacc ggctgcttgc tggcaaaggt gagcaccacc
     1501 gccatctgca gcagcacgcg gaaggtgtcg cggatattgt cggggtggaa ttcggcgaag
     1561 ctttcggcgc aatcgccgcc ctgcagcaaa aagcccttgc cggccgccac atcggccagc
     1621 tccgctttca gcgcgcgcgc ttcgccggca aacaccagcg gcggaaactt ggccagggtc
     1681 tgctccaccc ggccgagttc ctcggcgtcg ttatagaccg gaagatgccg cgcctcgcgg
     1741 gtcttccagc tgtcgggggt ccagttctgt gccacttgtc tcgctccttt cagaacgatg
     1801 gcacatagct accccgccgc cagaatccta gtgcatccgc atgcggcatt tgcgattact
     1861 ctgatctggc ggcgctgtgg ccgggttttt accgcgctca gcgggttttg gccccctctg
     1921 gcagcacttc cagccgccat gcccgccccg gcagaagata ctggccagcc agctgttgca
     1981 gccgttcggg cgtgacatcg gtgtaatcgg ccagcaggct gcgcagcgcg gcatagcggc
     2041 tggcatcacg cgtgccgccg gccacctggc ccatgaaaaa ggcggtgccg ctggtggcgc
     2101 gagcgatctg ctggcgcaag ggctcgatca cccgggccag atcatcggcc gagatggggc
     2161 gagcggccag atccgccgcg atcgcatcca ccgtggtgaa gaaatcgggc accttgtcgg
     2221 gctgcagctg ggccagtgcg gtcatcgtgc cgccattggc aaggtccagc ggccagtcgc
     2281 tggatacttg cggggcatag ctggcgcctt ccttctcgcg catggcatcg agcaggcggt
     2341 tggagaacac ctgcgagagc acttcgagct ggcgcgattc gcggatgccg gccaggccgc
     2401 cgccggtgct ccacgacact gccgccgcgg cctggttggc atcgcctgtg tggcgcagca
     2461 caatcggcgt gggcgtgggc cgggcggtgc ccacctgagt gggcgtggcg gccggcagat
     2521 cgccgcgcgg agccagcgcg ccaaagctgc gctgcagggc ggccacggcc ttgtcgcggt
     2581 cgaaatcgcc gaagatctgc acctcgatcg gcccctgcgc cagcaccggc gcccagcgcg
     2641 ccttgaagcc ggccacatcc accgccgcca gcgccgcctt ggaaggcacg ccaaagcgcc
     2701 agtcgccgcc gcgctgcagg gccttgagat cgcgattgag caccccctgc ggcgaggagg
     2761 cgaggctgtc atattgttgc agcgcggccg cccgggcgcg cgcaagcggc cctgcatccc
     2821 agcgcggctg ggcgaatttc tcggcgaaga ggtaaagctg gtcggccaga tcctcggcgc
     2881 gggtttccgc gctcagctgg aaattgccat catcgatgct gaaatcgaag cccatcttgc
     2941 ggccggtgga gatgctgtcc agctcctcct gccccagcgg cccctcgccc atgcccacca
     3001 gcgccatcgt gcccagcgtt gcataggcgg catcggccgg ctggaacgcg cgatagcccg
     3061 cgccaaagcg cacccgcacg cccacccggc ccggctcgtc atgcgcgggc cacagctgca
     3121 cgctcacgcc attggcaaag cgcagttcct cgatgccgag caggcccgag gggtgcgaca
     3181 ccggcgccgg cgcggcaggg ccaatcggcg gcagatcggc aaagctgatg gcatggctgc
     3241 ccgggcgggc atgggcatcg ggcgccacct tgccgcgcag ggccgcggcc agcgcgccgg
     3301 catcggcctc cgccgcatcg ggggtgacat agatcgcccg tgtcaccgcg cccgcaaaca
     3361 gcttgcgggt gtgttccagc accgcctcgg gcgtgaacag cggcagggat ttctgataga
     3421 cctccagcat gccctcgggc gaggtgacgg tctcgtggat atccagcgcg ccgaccagat
     3481 catcggccac gcgcgcgccc ggcagcaggc ggcgctgctc cacctggctt acgtagacga
     3541 cattgatctc ggccgcctcg cgggcgatct cctccttgct gggcggcgtg tcgagcgcat
     3601 cggcgatcac cgcgcgcact gccttcagcg ccgcgcgcca gtcgccatcg atcggcgtga
     3661 tggcggtgaa agtgccatcc agcgagcggc tttcattctc ctggcccacc tgcgcctgca
     3721 ggaaactggc gccagcgcgc gcgcgcgcct ccagccggcg gttgatgatg gccagcgcca
     3781 cctgatcgat catctggccc tggttgtaga cgatggtgtc gttcttctcg tgccaggggc
     3841 gcagggtggc aatggtgatc gcgcgctgct ggccgggggc caccaccacc cgcgtctcac
     3901 ccacaggatt ggccggatcg gcccccgcag gcgctgccgg cgcaccaaac gagggcaccg
     3961 gcacatgcgg ccccacgccc tgccaatcgc caaaccactt gccgatcaac gccgccatca
     4021 gccgcggatc ggcatcgccg ctgatgatga tggccgcgtt ttccggccga taccagcgct
     4081 ggtggaaagc gcgcacgccc tcctgatggg ccgcttccag caccgccacg ctgccgatcg
     4141 tggcatgatc gcccagcggc tggccggccc ccaccacttc gcgcatggca tcggcaatgc
     4201 gctccagctg gccgccgcgc tcgcgctttt cggccagcac gatgggcact tcggtgcgga
     4261 tgtcgctttc cgaaaggttg ggcgcgatca tcatgcccga cagcagcttc atgctttcat
     4321 caaggctggc cggcgtggca tcgggcaggt cgatcttgaa aacggtctgg gtcgggctgg
     4381 tctcggcatt ggtgtcggtg ccgaaagtgg cgcccaggcg ctgccaggtg ggaatcgcct
     4441 gcgcctggcc cagatatttc gactggcgga agaccagatg ctcgatcaga tgggcaaagc
     4501 cggcttcctc gggcttttcg aacatgctgc ccacatccat gcggatgcgg atggagacct
     4561 gccccggcgg caccccattg gcccgcaccg cccagcgcaa gccattggcc agcttgccga
     4621 atgtccactc tttatcgcgc gggacgtcgc tgttgacata gagccagggg ttggcctgat
     4681 cgggcagcgg cggcagcgcc gaaggggccg aggcccccgt ttgcgccgcc gccgccggcg
     4741 cgcgggcatg ggaagtgaca ggcaccagcg gcagggccag aacaatcgcg agggccgaga
     4801 cgaggcgcag cggcatacgg gcaggaaaac gcatgcgcag gatataggcc gcgcgaaaat
     4861 gaaggaccag tgaattgcat gccctgcgcc gtg
//
LOCUS       DASRYE010000098         6946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_129423,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000098 DASRYE010000000
VERSION     DASRYE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129423"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFF98_05460"
     CDS             <1..483
                     /locus_tag="VFF98_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZU63110.1"
                     /translation="HWQTGAAMPAAMAAKLKELRRVDQAYELGEVVAAALLDMAWHSQ
                     QAGSAPTDVAAFERQALAASGLDVAHVPPRYRSSLFRHIWSNDYAAGYYAYLWTEMLA
                     HDAFAWFEAHGGLTRANGQRFADCVLSRGHSADYGAMFRAFAGHDPDVAPMLAARGLK
                     "
     gene            complement(580..870)
                     /gene="rpmB"
                     /locus_tag="VFF98_05465"
     CDS             complement(580..870)
                     /gene="rpmB"
                     /locus_tag="VFF98_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZU63111.1"
                     /translation="MSRICELTGKGRQIGHNVSHANNKTKRVFLPNLQNVTLLSEGLD
                     RSFRFRVSTNGLRSVEHVGGLDNWLLKTSDTKLSPRAVKVKRELAKKAAVAA"
     gene            984..1463
                     /locus_tag="VFF98_05470"
     CDS             984..1463
                     /locus_tag="VFF98_05470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZU63112.1"
                     /translation="MTRWPLPDPMRRALAAAKAAAAAGEVPIGAVIVKGGTVISTAHN
                     LPRTRHDPTAHAEILAIRAAAAALGRERLEDCDIWVSLEPCPMCAGAIAHARLARLYY
                     AAPDPKGGGVAHGARVFDHPQCHHKPEVYAGIGEAEAAALLRDFFRQKRQAGPLTPP"
     gene            complement(1450..2313)
                     /gene="nadC"
                     /locus_tag="VFF98_05475"
     CDS             complement(1450..2313)
                     /gene="nadC"
                     /locus_tag="VFF98_05475"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZU63113.1"
                     /translation="MTAFAIPGFDLAAFVAATLAEDLGTGLAGGGHDVTSESVIPADA
                     RFAGVMDSRDAIVVAGLPIAAAFFRALDPAVEIELLVAEGARVAAGSALMRLAGNARA
                     LLTAERSALNTVQHLSGIATLTRQYVDAMGAAGARLLDTRKTIPGLRHLEKYATRMGG
                     AQNHRMGLWDAAMIKDNHVAVAGGVGPAVARARAAGVASIICEVDELGQIVPALEAGA
                     THLLLDNMGPAMLAEAVALVAGRVPCEASGGVRLDTIGAIAAAGVDYVSVGRLTQSAP
                     AADIGLDFKAV"
     gene            complement(2334..3140)
                     /locus_tag="VFF98_05480"
     CDS             complement(2334..3140)
                     /locus_tag="VFF98_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HZU63114.1"
                     /translation="MTIRTILVDDEKLAIQGLQLRLAKFPDVEIIDTCNNGREAIRKI
                     KTEKPDLVFLDIQMPGFDGFSVVKGVMEIEPPLFVFVTAYQEHAVRAFEANAVNYLMK
                     PVDEDRLADTLDRVRQRLADKRLVEEAEKLKTVLAEVAPDAIDQMPEPTEAEPAGGAR
                     YERLINIKDRGQIFRVDVDTIEHIEAAGDYMCIRTADNSLILRETMKDLERRLDPRVF
                     QRVHRSTIVNLNLVRQVKPHTNGECFLVLESGAQVKVSRSYRDVVARFVH"
     gene            complement(3206..4360)
                     /locus_tag="VFF98_05485"
     CDS             complement(3206..4360)
                     /locus_tag="VFF98_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU63115.1"
                     /translation="MAMLPFQPTPFFANKNKAFWRLQVLGWSGAMLLRALSSLANGQP
                     PAFLVLVLIATINGFSISLILAVVYHQLIGKRPLLTWGATAVILPLAVGLCAFMDGWV
                     IALYRPDSAASFAQLVVGVFYLDATLLGAWSALYYAINFFLRVEEQADQLIRLESQAT
                     SAQLAMLRYQLNPHFLFNTLNSISTLVLLKQTEAANAMLTRLSSFLRYTLVNEPAARV
                     TVAQEVETLELYLGIELMRFEERLRTTFRIDPACEEALLPNLLLQPLVENAIKYAVSP
                     QETGAEITIAASQIGQKLHITVSDTGPGLQGPHSENRLSGMSFDGGEPVSTGVGLANI
                     RDRLAQAYRFDHLFETWEPPEGGFAVTIEIPFETRAQAAAAGGPRAPGAG"
     gene            complement(4373..5104)
                     /locus_tag="VFF98_05490"
     CDS             complement(4373..5104)
                     /locus_tag="VFF98_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZU63116.1"
                     /translation="MADAANRSTPWPLIGALCLMGAGLGVLAGRQWAYSAMGDRAAME
                     QVVHDYILAHPEILPQAVEKLHQQEAGQQLAALRPALESPFPGAVIGNPAGRRVIVEF
                     MDFACGYCRQSVADVDALVAAHPDVKVVVRQLPILTPASADAAKMALAAAEQGKYPAF
                     HRAMFAAGRPDGQSIAAAARAAGLDWPRAQAYAATPGVAAEIARNQQMAQQLGMNGTP
                     SWVVGGQLLGGALGREALENALAAM"
     gene            complement(5140..6513)
                     /locus_tag="VFF98_05495"
     CDS             complement(5140..6513)
                     /locus_tag="VFF98_05495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZU63117.1"
                     /translation="MPRRLRAGRLGLGLLLGLLAAPPALAQSILRDAETEALFHDMTS
                     PLVAAAGLDTRNVDIVLVNDPEINAFVAGGQAVYVNSGLINEASSANEVQGVVAHELG
                     HVVGGHAIDERGAKQAGNISLLSLLLAGAAAAAGGGAAAMGVFMAGQQAAMGSYLSYT
                     RGEEEAADAAGAKFLSTAGISGRGSVEFFHKLLNLENRYGLTRTEEDSFWSTHPMTDD
                     RIQFLQDTYEKDPAWNKPKDPQLEARFQRVKAKLYGYLAEPADTLRAYPEYMTGDPAR
                     YARAYAWHKEGFLDKAMGETDALLAHAPDDPYFLELKGQILLEAGKPAEALEPLRRAT
                     QLTNNQPLIATTFGHALMALDESDPNHPHLAEARRVLKAAVARDRENPFAWYVLGEVY
                     SAMGDTPRARLASAEQQSLSGNMVEAMRNAQAALAALPPGSADALRAQDIAMEARTAM
                     ARRKKGK"
     gene            complement(6607..>6946)
                     /locus_tag="VFF98_05500"
     CDS             complement(6607..>6946)
                     /locus_tag="VFF98_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63118.1"
                     /translation="AARAALPFEAEGPAPADGAGRGALFVYRGVLLPFAGEDGAVCRV
                     LAVLGWKEVAEPSLAATLMAELAADLAGLALAEPLLPADPAAHVLGWEALMAEAPAAA
                     PALLRLRQIA"
BASE COUNT         1159 a         2434 c         2252 g         1101 t
ORIGIN      
        1 cactggcaaa ccggcgctgc catgcccgcc gccatggccg ccaagctgaa ggagctgcgc
       61 cgggtggatc aggcctatga gctgggcgag gtggtggccg cagccctgct cgacatggcc
      121 tggcacagcc agcaggccgg cagcgccccc accgatgtcg ccgcttttga acgccaggcg
      181 ctggccgcca gcgggctgga tgtggcgcat gtgccgccgc gctatcgctc cagcctgttc
      241 cgccacatct ggtcgaacga ttatgccgcc ggctattacg cctatctgtg gacggagatg
      301 ctggcccatg acgccttcgc ctggttcgag gcgcatggcg gcctgacccg cgccaacggg
      361 cagcgctttg ccgattgcgt gctcagccgc gggcattcgg ccgattatgg cgcgatgttc
      421 cgcgcctttg ccgggcatga tccggatgtg gcgccgatgc tggccgcgcg cgggctgaaa
      481 tagcccgccg gccccgggcg ctggcgccca cagcaaaagg cgcgaggggg ctcaaacgcc
      541 accccgcgcc tttttggctg aaagcctgtg cgctgcctgt caggcggcga cagcggcctt
      601 cttggccagc tcgcgcttca ccttcacggc gcgcgggctg agcttggtgt ccgaggtctt
      661 cagcagccag ttgtcgaggc cgccaacatg ctcaaccgaa cgcaggccgt tggtcgacac
      721 gcggaaccgg aagctgcgat ccaggccttc cgacagcagg gtaacgttct gcaggttggg
      781 caggaacacg cgcttggtct tgttgttggc gtggctgacg ttgtggccga tctggcgacc
      841 cttgccggtc agttcgcaaa tacgggacat gggcttctcg cttgaaaatc gtggttccgt
      901 tgccggaaga aggggggccc ttaccgattc ggtgccgaat cgtcaagttg cgcatgtgcc
      961 ttccacgcgc tagcgctgca ggtatgaccc gctggccgct tcccgatccc atgcgccggg
     1021 cgcttgccgc cgccaaagcc gccgctgcgg ccggcgaagt gcccatcggc gcggtgatcg
     1081 tcaagggcgg cacggtgatt tccacagccc acaacctgcc gcgcacccgc cacgatccca
     1141 ccgcccatgc cgagatcctg gcgattcgcg ctgccgctgc cgcgcttggc cgcgaaaggc
     1201 tggaagattg cgacatatgg gtaagcctgg agccctgccc gatgtgcgcg ggcgccatcg
     1261 cccatgcccg cctcgcccgg ctctactatg ccgcgcccga tcccaagggc ggcggcgtgg
     1321 cccatggcgc ccgcgtgttc gaccaccccc agtgccacca caagccggaa gtctatgccg
     1381 gcatcggcga ggcggaagcg gccgcgctgc tgcgcgattt cttccgccag aagcgccagg
     1441 ccgggcccct tacaccgcct tgaaatccag cccgatatcg gccgccggcg cgctctgcgt
     1501 cagccgcccc accgagacat aatcgacgcc cgccgcggca atcgccccga tcgtgtcgag
     1561 ccgcacgccg ccgctcgcct cgcaaggcac ccggccagcc accagcgcca ctgcctcggc
     1621 cagcatcgcg gggcccatat tgtcgagcag cagatgggtg gcgcccgctt ccagcgccgg
     1681 cacaatctgg cccagctcgt ccacctcgca gatgatgctg gccacgcccg ccgccctggc
     1741 ccgcgccacc gccggcccca cgccgccggc caccgccacg tgattgtcct tgatcatcgc
     1801 cgcatcccac aggcccatgc ggtggttttg ggcgccgccc atgcgggtgg catatttttc
     1861 cagatgccga aggcccggga tggtcttgcg cgtgtcgagc aggcgggccc cggccgcgcc
     1921 catggcatcg acatactggc gcgtcagcgt ggcgatgccc gagaggtgct gcacggtgtt
     1981 gagcgcgctg cgctcggccg tcagcagagc gcgggcattg ccggcaaggc gcatcagcgc
     2041 gctgcccgcc gccacccgcg cgccttctgc caccagcagc tcgatctcca ccgccggatc
     2101 cagcgcgcgg aagaaggccg cggcaatcgg caggccggcc accacgatgg catcgcggct
     2161 gtccatcacg ccggcaaagc gcgcatcggc agggatcacg ctttcgctgg tgacatcatg
     2221 gccgccgccg gcaaggcccg tgcccagatc ctcggcaagg gtggcagcga caaaggcggc
     2281 aaggtcgaaa cccggaatgg caaaggcggt catgccgcag cccttagcct gccttagtgc
     2341 acgaagcgag ccaccacatc gcgataggac cgcgaaacct tcacctgcgc gcccgattcc
     2401 agcacaagga aacattcgcc attggtatgc ggctttacct gccgcaccag attgaggttg
     2461 acgatggtgg agcggtgcac ccgctggaac acgcgcggat ccagccggcg ttccagatcc
     2521 ttcatcgtct cgcgcaggat cagcgaattg tcggcggtgc ggatgcacat gtaatcgccc
     2581 gccgcctcga tatgctcgat cgtgtccaca tcgacgcgga agatctggcc gcgatccttg
     2641 atgttgatca gccgctcata gcgcgcgccg ccggcaggct cggcctccgt gggctcgggc
     2701 atctggtcga tggcatcggg cgccacttcg gccagcaccg tcttcagctt ttccgcctct
     2761 tccaccaggc gcttgtcggc caggcgctgg cgcacccggt cgagcgtgtc ggcgaggcga
     2821 tcctcgtcca ccggcttcat cagatagttc accgcattgg cctcgaaggc gcgcacggca
     2881 tgttcctgat aggcggtgac gaaaacgaac agcggcggct cgatctccat cacccccttc
     2941 accaccgaga agccatcgaa gccgggcatc tggatgtcga ggaaaacaag gtcgggcttt
     3001 tcggtcttga tcttgcggat ggcctcgcgg ccattgttgc aggtgtcgat gatctcgaca
     3061 tcgggaaact ttgccaggcg cagctgcagc ccctgtatcg ccagcttctc atcgtcgacg
     3121 aggatggtgc ggatggtcat gggggatggt cctgtatcga agaagggtaa ggatgtggcc
     3181 ccaagcgggc cgattgcaag caccttcagc cggcaccggg cgcacggggg ccaccggccg
     3241 ccgcagcctg ggcgcgggtt tcgaagggaa tctcgatggt cacggcaaag ccgccctcgg
     3301 gcggctccca ggtttcgaag aggtgatcga accgataggc ctgggccagc cggtcgcgga
     3361 tattggccag gccaacgccg gtggaaaccg gctcgccgcc atcaaagctc atacccgaga
     3421 ggcggttttc gctgtggggc ccctgcaggc ccggcccggt atccgacacg gtgatatgca
     3481 gcttctggcc gatctggctg gcggcaatgg tgatctcggc gccggtttcc tgcggcgaga
     3541 cggcatattt gatggcgttt tccaccaacg gctgcagcaa cagattgggc agcagcgcct
     3601 cctcgcaggc cggatcgatg cggaaagtgg tgcgcagccg ctcctcaaag cgcatcaact
     3661 caatgccgag ataaagctcc agcgtctcca cttcctgcgc caccgtcacg cgggcggccg
     3721 gctcgttcac cagcgtgtag cgcaggaagg aggagaggcg cgtgagcatc gcattggccg
     3781 cttccgtctg cttcagcagc accagggtgg aaatggaatt gagcgtgttg aacaggaaat
     3841 gcgggttcag ctgatagcgc agcatggcca gctgggcgct ggtggcctgg ctttccaggc
     3901 ggatcagctg gtcggcctgc tcttccacgc gcaggaagaa gttgatcgcg taatacagcg
     3961 ccgaccacgc ccccagcagc gtggcatcca gatagaacac gccgaccacc agctgggcaa
     4021 agctggccgc gctgtcgggc cgatagagcg cgatcaccca gccatccatg aaagcgcaca
     4081 ggcccaccgc cagcggcagg atcaccgccg tcgcccccca ggtgagcagc ggccgcttgc
     4141 cgatcagctg gtgatagacc accgccagaa tgagcgagat ggaaaagccg ttgatcgtgg
     4201 cgatcagcac cagcaccaga aaggccggcg gctgcccatt ggccagcgac gacaacgccc
     4261 tgagcaacat cgcgcctgac cagccgagca cttgcaggcg ccagaaagcc ttgttcttgt
     4321 tagcgaaaaa cggtgtcggc tggaacggaa gcatggccat ggggcaatcg gcctacatcg
     4381 ccgccagcgc gttttccagc gcctcgcggc ccagcgcgcc gcccagcagc tggccgccca
     4441 ccacccagct gggcgtgccg ttcatgccca gctgctgggc catctgctgg ttgcgggcga
     4501 tctctgcggc aacgccgggc gtagcggcat aggcctgcgc gcgtggccaa tcgagcccgg
     4561 ctgcgcgcgc ggctgccgca atgctctgcc cgtcgggccg gccggcggcg aacatggcgc
     4621 ggtggaaggc gggatatttg ccctgttcgg ccgctgccag cgccattttg gcggcatcgg
     4681 cgctggccgg cgtcaggatc ggcagctggc gcaccaccac cttcacatcc ggatgggccg
     4741 ccaccagcgc atccacatcg gccacgctct gccggcaata gccgcaggca aaatccatga
     4801 actccacaat cacccgccgc cccgccggat tgccgatcac cgcgccgggg aagggcgatt
     4861 ccagcgccgg gcgcagggcg gccaattgct ggccggcttc ctgctggtgc agcttttcca
     4921 ccgcctgcgg caggatttcg ggatgggcga ggatatagtc atgcaccacc tgctccatgg
     4981 cggcgcgatc gcccatcgcc gaataggccc actgccgccc ggccagcacc cccagccccg
     5041 cccccatcag gcacagggcg ccgatcaacg gccatggtgt ggaacgattg gcggcatcgg
     5101 ccatgaaaaa aactcctcct cctgcgcagg gcgggcagct tacttgcctt ttttccgccg
     5161 ggccatagcc gtgcgggcct ccatggcgat atcctgcgcg cgcagggcat cggcagagcc
     5221 cggcggcagg gcggcaaggg cggcctgcgc attgcgcatc gcctccacca tattgcccga
     5281 cagcgattgc tgctcggccg aggcgaggcg cgcgcgcggc gtatcgccca tggcgctgta
     5341 gacctcgccc agcacatacc aggcaaaggg gttctccctg tcccgcgcga cagccgcctt
     5401 cagcacccgg cgcgcctcgg ccagatgggg gtggttgggg tcgctctcgt ccagcgccat
     5461 cagcgcatgg ccgaaagtgg tggcgatcaa cggctggttg ttggtcagct gggtggcccg
     5521 gcgcagcggt tccagcgcct cggcgggctt gccggcctcc agcaggatct ggcccttcag
     5581 ttccaggaaa tagggatcat cgggcgcatg ggccagcagc gcatcggtct cgcccatcgc
     5641 cttgtcgagg aagccctcct tgtgccaggc ataggcgcgg gcatagcggg ccggatcgcc
     5701 ggtcatatat tcgggatagg cgcgcaacgt gtcggccggc tctgccagat agccatagag
     5761 cttggccttc acccgctgga aacgcgcttc cagctgcggg tccttcggct tgttccaggc
     5821 cgggtccttc tcgtaggtgt cctgcaggaa ctggatgcgg tcgtccgtca tcgggtgcgt
     5881 cgaccagaag ctgtcttcct cggtgcgggt gaggccatag cgattttcca gattcagcag
     5941 cttgtggaag aattccaccg agccgcgccc ggaaatgccc gcggtggaga ggaatttggc
     6001 gccagccgca tcggccgcct cttcctcgcc gcgggtatag gacagatagc tgcccatcgc
     6061 cgcctgctgg ccggccatga acacgcccat cgccgccgcg ccgccgccgg ccgccgccgc
     6121 cgcgcccgcc agcagcagcg acagcagcga gatattgccg gcctgcttgg cgccgcgctc
     6181 gtcgatcgca tggccgccca ccacgtggcc caactcgtgc gccaccacgc cctgcacctc
     6241 attggcgctg ctggcctcgt tgatgaggcc gctgttgaca tagaccgcct gcccgccagc
     6301 gacaaaggcg ttgatctccg gatcgttaac gagaacgata tcgacattgc gcgtgtcgag
     6361 cccggcggcg gccaccagcg ggctggtcat gtcgtgaaac agcgcctcgg tctcggcatc
     6421 gcgcaggatg gattgcgcca gcgccggcgg cgcggccagc agccccagca acaggcccag
     6481 ccccagccgc cccgcacgca ggcgccgggg caacaaaagg gcatgtcgaa cggcaagaat
     6541 cgcaggcgca gtcacaggcc agcgctagca catagctggc tgaatggcgc ctgaagcctg
     6601 ccgcccctag gcaatctgcc gcaagcgcag cagggccggc gccgcagcgg gcgcctcggc
     6661 catcagcgct tcccagccca gcacatgcgc cgccggatcg gccggcagca gcggctctgc
     6721 cagcgccagc ccggccagat ccgccgccag ttcggccatc agcgtggcgg ccagcgatgg
     6781 ctctgccact tccttccagc ccagcaccgc cagcacccgg cacaccgcgc catcctcccc
     6841 ggcaaagggc agcagcacac cgcgatagac aaacagcgcc ccgcggcccg ccccatcggc
     6901 aggcgcgggc ccctcggcct cgaacggaag ggccgcgcgg gccgcc
//
LOCUS       DASRYE010000099         6705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_162444,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000099 DASRYE010000000
VERSION     DASRYE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162444"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFF98_05505"
     CDS             <1..332
                     /locus_tag="VFF98_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU63119.1"
                     /translation="TRARLRAEYDARLSAVVGEVEALLAQYAQISAQLERLRQELPAA
                     RTAARQAAAALHAGTIDERAFADLAANRLAREQEFTSLQTAALDRQIALQTLTGDGLP
                     TITGTPQ"
     gene            329..1369
                     /locus_tag="VFF98_05510"
     CDS             329..1369
                     /locus_tag="VFF98_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63120.1"
                     /translation="MNRPALLAPALLAPALLALAGCSAADAPADPQPSVAVSTARVVR
                     GALPETVIAYGTASPSPAGVETLGFAQPGQITAVMVSAGLPVRKGQPLLTFAPAASVR
                     ASYAQAQNALHAAQVQESATRQLLARQLATTDQLAQAERALADARASLQGLAADSAGS
                     ASTTLTAPFDGVVATLGIARGERSPAGAPLLTLARADALIVSAGLDPADSAHVSAGQG
                     VQLQRLSGGTQVAGHVLRVAGALDPKTRLVPVEIAFPAGALMPGEAVRAQIATRPVAG
                     WLVPHRAVVTADGPPHVFEDRAGKASAVPVAILLATDSGDVVDGALDPARPVITDGAY
                     QLRDGDRLRRGQ"
     gene            1369..4404
                     /locus_tag="VFF98_05515"
     CDS             1369..4404
                     /locus_tag="VFF98_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63121.1"
                     /translation="MLETLLRTQSRAFVLIAMAAALAGLVAAFGLPVGLFPQVAFPRV
                     VVDLDSGSRPADQTALAVTRPVEEAIRALPGVQDVRSETSRGTAQISIDFGWGRDMVA
                     STLLVDTAIARILPGLPAGTQYNVRRMDPTVFPIISYALESDRDPRALQDLAKYQITP
                     LLSSIPGLARVGVQGGETSEVEVLADPHRLADHGMALADLAQAIRAGNVLSAVGQVQD
                     RGRLSLVIADHSAVNSAQIGDIVVKADPAGVVRVRDVASVQDGSVPQWLRIVEDGRPA
                     VLFNIYEQPDGNAVQIAQDVHQRLQALRLPPGVRLVNWYDQSELVTQSAASVRDAVLI
                     GLVLAAIVLLAFLKSWRVTLVAVIVVPATLAATVLVLAVLGMSFNIMTLGGIAAAVGL
                     LIDDVIVMVEHIARRAGAPSADGTPMGAAAVLPAAREFMAPLMGSSLATLIVFLPLSF
                     LTGVTGAFSKALSITMAAALAISWAMTAFVVPVLVRALVDFETWHDPAAGADGRLERA
                     HGALLARLSRRPALLLAAAAVLLGLGYAGYANVPTGFMPRVDEGGFVMDYYTPPGTSL
                     DETGRQVGQIDALLRANRDVLTFSRRLGTGMGGDLGQTYHGDYFVKLKPGHARPTEEV
                     AAAIAAEVARQVPGVDVEVAQLMEDLIGDLTAVPQPIEVKLFAADPAVLYGQAQKVAG
                     LISAIPGVIEVKDGVRLAGDALDVRIDPVRAGLESVAPADIEAQMNAALFGTVATSLA
                     QPGKAVDVRVRLPEALRLSQADLAHLPIRATDGHVFPLGRVATIAAVAGQPQIARENL
                     EPMVAVTGRIQGRGMGAAVADVTRLLDRPGTLAPGVRYELGGLYQQQQIAFNGLIRVF
                     GAALMAELILLVVLYRGLVLPLIIIGCALLSTTAVFTGLWLTGVDLNITALMGMTMII
                     GIGTEMAIFYVSEYEELAGHMPRGEAAWQAARGRLRPITMTTLAAILTLLPLAFAIGQ
                     GSGIQQPLAIAIIAGLLLQYPLVLLVMPVLVRLALPGQR"
     gene            complement(4505..4594)
                     /locus_tag="VFF98_05520"
     tRNA            complement(4505..4594)
                     /locus_tag="VFF98_05520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4558..4560),aa:Ser,seq:cga)
     gene            4751..5131
                     /gene="hspQ"
                     /locus_tag="VFF98_05525"
     CDS             4751..5131
                     /gene="hspQ"
                     /locus_tag="VFF98_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HZU63122.1"
                     /translation="MNRATFFSPQAGRQISAPEASRARFGIGDVVRHRQFGFRGVIFD
                     IDPVFANSEEWYESIPQNSRPPREQPFYHLFAEGEEASYIAYVSQQNLVADGQAGPVD
                     HPSLPQIFETFDSGRYRLRQRLSH"
     gene            complement(5164..6027)
                     /locus_tag="VFF98_05530"
     CDS             complement(5164..6027)
                     /locus_tag="VFF98_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63123.1"
                     /translation="MAAPQATARVTPALAQFVPHGQPIIHGVNRLGLWTLYMKEVRRF
                     FKVQTQTIWAPAITTLLFLVIFTVAMGRGNRMVLGVNFATFVAPGLIVMGMMQNAFAN
                     ASFSFLAGKIQGTIVDYLMPPLSTGELMLALAGGAVTRGVLVGLALAGAMLVWPGVSL
                     SVAHPWAVVWFGLMGTLMLAFVGLLSSIWAEKFDHNAAVTNFVIQPLSLLSGTFYVID
                     NLAPSFQAVSRANPFFYVISGFRYGFLGQSDIGTTNAQVVQGALVLALLNGLLGALVY
                     GLLRRGWKLKS"
     gene            6124..>6705
                     /locus_tag="VFF98_05535"
     CDS             6124..>6705
                     /locus_tag="VFF98_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HZU63124.1"
                     /translation="MSWTDERIEKLTKMWEGGATASQIAEELGGVSRNAVIGKAHRLG
                     LKSRPSPVKANAPGEEPESAPAPRPAPRPPEASPPPPPVQAAPSPAPRPAPPAPAPQA
                     AAPAPAPAASTAPAAPASDSPPAPRIVSVGPGGFLRQGPGEQQAPIPPAPPRRLVPAK
                     PSPDIADKTSLLDLNDRICRWPVGHPGEPDFHFC"
BASE COUNT         1003 a         2418 c         2274 g         1010 t
ORIGIN      
        1 ccacccgcgc ccgcctgcgc gccgaatatg acgcgcgcct gtcggccgtg gtgggcgagg
       61 tggaggcgct gctggcccaa tacgcccaga tcagcgccca gctggaacgc ctgcgccagg
      121 aactgcccgc cgcccgcacc gccgcccgcc aggctgcagc ggccctccac gccggcacga
      181 tcgacgagcg cgcctttgcc gatctggccg ccaaccgcct ggcccgcgaa caggaattca
      241 ccagcctgca aaccgcagcg ctcgaccgcc agatcgccct acagacgctg actggcgacg
      301 gcctgcccac catcaccgga accccgcaat gaaccgcccc gccctgcttg ctcccgccct
      361 gctcgcccct gccctgctgg cccttgccgg ctgcagcgcg gccgatgcgc ctgccgatcc
      421 ccagcccagc gtcgctgtca gcaccgcgcg ggtggtgcgc ggcgcgctgc ccgaaaccgt
      481 tattgcctat ggcaccgcca gcccctcgcc cgccggggtg gaaacgcttg gctttgccca
      541 gcccgggcag atcacagcgg tgatggtctc ggccgggctg ccggtgcgca agggccaacc
      601 gctgctcacc ttcgcgccgg ccgccagcgt gcgcgccagc tatgcccagg cgcagaacgc
      661 gctgcatgcc gcgcaggtgc aggaaagcgc cacccggcag ctgctcgccc ggcaactggc
      721 caccaccgac cagctggcgc aggcagagcg ggcccttgcc gatgcccgcg ccagcctgca
      781 gggcctggcc gccgatagcg ccggcagcgc cagcaccacc ctcaccgccc ccttcgatgg
      841 cgtggtggca acgcttggca tcgcccgggg cgagcgcagc ccggccggcg ccccgctgct
      901 caccctggcc cgcgccgacg cgctgatcgt gagcgccggc ctcgacccgg ccgacagcgc
      961 ccatgtcagc gccgggcagg gcgtgcagct gcagcgcctg tcgggcggga cgcaggtggc
     1021 gggccatgtg ctgcgcgtgg ccggcgcgct cgatcccaaa acccggctcg tcccggtcga
     1081 gatcgccttt cccgccggcg cgctgatgcc cggcgaagcg gtgcgggccc agatcgccac
     1141 gcggccggtg gccggctggc tggtgccgca ccgggccgtg gtgacggccg atggcccccc
     1201 gcatgtgttc gaggacaggg cgggcaaggc cagcgccgtg ccggtggcca tcctgctggc
     1261 caccgatagc ggcgatgtgg tggatggcgc gctcgatccg gcgcggccgg ttatcaccga
     1321 tggcgcctat caactgcgcg atggcgatcg cctgcggcgg gggcagtgat gctggagact
     1381 ttgctgcgca cgcaatcgcg cgctttcgtg ctgatcgcca tggccgccgc gctggccggg
     1441 ctggtggccg cctttggcct gccggtgggc ctgttcccgc aagtcgcctt cccgcgcgtg
     1501 gtggtcgatc tcgattccgg cagccgcccg gccgaccaga ccgcgctggc cgttacccgc
     1561 ccggtggagg aagccatccg cgccctgccc ggcgtgcagg atgtccgttc cgaaaccagc
     1621 cgcggcacgg cgcagatttc catcgatttc ggctggggcc gcgacatggt ggccagcacg
     1681 ctgctggtcg ataccgccat cgcgcggatc ctgccgggcc tgcccgccgg cacgcaatac
     1741 aatgtgcggc ggatggaccc gacggtgttc ccgatcatct cctatgcgct ggaatccgac
     1801 cgcgacccgc gcgcgctgca ggatctggcc aaatatcaga tcaccccgct gctctcctcc
     1861 atcccgggcc ttgcccgcgt gggcgtgcag ggcggcgaga ccagcgaggt ggaagtgctg
     1921 gccgatcccc accgcctggc cgaccatggc atggcgctgg ccgatctggc gcaggcgatc
     1981 agggcgggca atgtgctgtc cgctgtgggc caggtgcagg atcgcgggcg cctctcgctg
     2041 gtgatcgccg accacagcgc ggtgaacagc gcccagatcg gcgatatcgt ggtgaaagca
     2101 gaccccgccg gcgtggtgcg cgtgcgcgat gtggccagcg tgcaggatgg cagcgtgccg
     2161 caatggctgc gcatcgtgga agacgggcgg ccggcagtgc tgttcaacat ctatgaacag
     2221 cccgatggca atgccgtgca gatcgcgcag gatgtgcacc agcgcctgca ggccctgcgc
     2281 ctgccccccg gcgtgcgcct cgtcaactgg tacgaccaga gcgagctggt gacgcaatcg
     2341 gcggccagcg tgcgcgatgc ggtgctgatc gggctggtgc tggccgcgat cgtgctgctg
     2401 gccttcctga agagctggcg cgtcacgctg gtggcggtga tcgtggtgcc ggcaacgctg
     2461 gccgccacgg tgctggtgct ggcggtgctg ggcatgagct tcaacatcat gacgctgggc
     2521 ggcatcgccg cggccgtggg cctgctgatc gacgatgtga tcgtgatggt ggaacatatc
     2581 gccaggcgcg ccggcgcgcc cagcgcggat ggcacgccca tgggggcggc cgccgtgctg
     2641 cctgccgcgc gcgaattcat ggcgccgctg atgggctcca gcctggccac gctaatcgtc
     2701 ttcctgccgc tctccttcct caccggggtc accggggcct tttccaaggc gctgtcgatc
     2761 accatggcgg ccgctcttgc catcagctgg gcgatgaccg ctttcgtggt gccggtgctg
     2821 gtgcgcgcgc tggtcgattt cgagacgtgg cacgatccgg ccgccggcgc cgatggccgg
     2881 ctggagcgcg cccatggcgc cctgctggcc cgcctgtcgc gccggccggc gctgctgctg
     2941 gcggcagctg cggtgctgct ggggctgggc tatgccggct atgccaatgt gcccaccggc
     3001 ttcatgccca gggtggatga gggcggcttc gtgatggatt actacacgcc gccgggcacc
     3061 tcgctggatg aaaccgggcg ccaggtgggc cagatcgatg cgctgctgcg cgccaaccgg
     3121 gatgtgctca ccttctcgcg ccggctgggc acgggcatgg gcggcgatct cgggcagacc
     3181 tatcatggcg actatttcgt caagctgaag cccggccatg cccggcccac agaagaggtg
     3241 gcggccgcca ttgccgccga agtggcgcgg caggtgcccg gtgtcgatgt cgaggtggcg
     3301 cagctgatgg aggacctgat cggcgacctg accgccgtgc cccagccgat cgaggtcaag
     3361 ctgttcgcgg ccgatcctgc cgtgctttat gggcaggcgc aaaaggtggc cgggctcatc
     3421 tcggccatcc ccggggtgat cgaggtgaag gatggcgtgc ggctggccgg cgatgcgctg
     3481 gacgtgcgga tagacccggt gcgcgccggg ctggaaagcg tggccccggc cgatatcgag
     3541 gcgcagatga atgccgccct gttcggcacg gtggccacct cgctggccca gccgggcaag
     3601 gcggtggatg tgcgcgtgcg cctgcccgag gcgctgcgcc tcagccaggc cgatctggcc
     3661 cacctgccga tccgcgccac agatggccat gtcttcccgc tgggccgggt ggccacgatc
     3721 gcggcggtgg ccggccagcc ccagatcgcg cgtgagaacc tggagccgat ggtggccgtg
     3781 accggccgca tccaggggcg cggcatgggc gcggcggtgg ccgatgtgac acggctgctg
     3841 gaccggcccg gcacgctggc ccccggcgtg cgttatgaac tgggcggcct ctaccagcag
     3901 cagcagatcg ccttcaacgg gctgatccgg gtgttcggcg cggcgctgat ggccgagctg
     3961 atcctgctgg tggtgctcta tcgcgggctg gtgctgccgc tcatcatcat cggctgcgcg
     4021 ctgctgtcca ccacggcggt gttcacgggg ctgtggctga caggggtgga tctcaacatc
     4081 accgcgctga tggggatgac gatgatcatc ggcatcggca ccgaaatggc gatcttctac
     4141 gtgtcggaat atgaggaact ggccggccac atgccgcgcg gcgaggccgc ctggcaggcc
     4201 gcgcgcggcc gcctgcggcc gatcaccatg accacgctgg ccgccatcct caccctgctg
     4261 ccgctggcct ttgccatcgg ccagggatcg ggcatccagc aaccgctggc cattgccatc
     4321 atcgccgggc tgctgctgca atatccgctg gtgctgctgg tgatgccggt gctggtgcgc
     4381 ctggccctgc ccggccagcg ctgaggaagg cccaaaagcc ccgggcggcg ctggctgccc
     4441 ggggctttgg cgcagcccgg caatgccggc ttatccctgc aaaatcagca tgtctgtaag
     4501 gaagtggcgg agaggcaggg attcgaaccc tggataccct tgcgagtatg ccgcatttcg
     4561 agtgcggtgc attcgaccac tctgccacct ctccgcgagg tcttcggccg gggctgcaaa
     4621 gcggctccgg ccaggtcagg agcgcgcccc tagcccatgg cgcgcacctt gccaaccccc
     4681 aaatgcggct ggcttgcgaa ttttaccggc ctcgcttgtg ggcccgggct gcccccccta
     4741 tatttgcctc atgaatcgtg cgaccttctt ctcgccccag gccggccggc agatctcggc
     4801 ccccgaggct tcccgtgcgc gctttggcat tggcgatgtg gtgcgccacc ggcaattcgg
     4861 ctttcgcggg gtcatcttcg atatcgatcc ggtcttcgcc aacagcgagg aatggtacga
     4921 atcgatcccg cagaacagcc gccccccgcg cgagcagccc ttctatcacc tcttcgccga
     4981 gggcgaggaa gccagctaca tcgcctatgt cagccagcag aatctggtgg ccgacgggca
     5041 ggccggcccg gtcgatcacc cctcgctgcc gcagatcttc gagacttttg acagcgggcg
     5101 ctatcgcctg cgccagcgcc tcagccactg agcacgggag cggggccctg aggccccttc
     5161 ccgtcagctc ttcagcttcc agccccggcg cagcaggcca tacaccagcg cccccagcag
     5221 cccgttcagc agcgccagca ccagcgcgcc ctgcaccacc tgcgcattgg tggtgccaat
     5281 gtcgctctgc cccagaaagc catagcggaa gcccgagatc acatagaaaa acgggttcgc
     5341 ccggctgacc gcctgaaacg agggggccag attgtcgatc acataaaacg tgcccgacag
     5401 cagcgagagc ggctggatga caaaattggt gaccgccgcg ttatggtcga acttttccgc
     5461 ccagatcgac gacagcaggc cgacaaaagc cagcatcagc gtgcccatca ggccaaacca
     5521 caccaccgcc caggggtgcg ccaccgagag ggacaccccc ggccacacca gcatcgcgcc
     5581 ggccagcgcc agccccacca gcacgccgcg cgtcaccgcg ccgcccgcca gcgccagcat
     5641 cagctcgcct gtggacagcg gcggcatcag ataatccaca atggtgccct ggatcttgcc
     5701 ggccaggaaa ctgaagctgg cattggcaaa ggcattctgc atcatgccca tcacgatcag
     5761 cccgggcgcc acgaaagtgg cgaaattcac ccccagcacc atgcggttgc cgcgccccat
     5821 cgccacggtg aaaatcacca gaaacaacag ggtggtgatc gccggcgccc agatcgtctg
     5881 ggtctgcacc ttgaagaaac ggcgcacctc tttcatatag agggtccaca ggcccagccg
     5941 gttcaccccg tggatgatgg gctggccatg cggcacaaac tgcgccagag ccggagtaac
     6001 acgcgcggtg gcctgggggg cggccatgtg gggttgatca gccatgctgc ggcgactatc
     6061 ggggtctatc gcccaaggca agcggcgcga gggcccgtgg ggcccctttt gaatggaata
     6121 tcgatgagct ggaccgacga acgcatcgaa aagctgacca agatgtggga gggcggtgcc
     6181 accgcctcgc agatcgccga ggaattgggc ggcgtctcgc gcaatgccgt gattggcaag
     6241 gcccaccggc tgggcctcaa atcgcgccca tcgccggtga aggccaatgc ccccggcgag
     6301 gaaccggaga gcgcgcccgc gccccggcca gcgccgcgcc cacccgaggc cagcccgcca
     6361 ccgccgccgg tgcaggcagc gcccagcccg gccccgcgcc cggcgccccc tgcccctgcc
     6421 ccgcaggcag cagcccccgc gccggcaccc gccgccagca cggcccctgc cgcgcccgcc
     6481 agcgattcgc cgccggcgcc gcgcatcgtc tcggtggggc ccggcggctt cctgcgccag
     6541 ggcccgggcg aacagcaggc ccccatcccg ccggcgccgc cgcgccgcct ggtgccggcc
     6601 aagcccagcc ccgacatcgc cgacaagacg agcctgctcg atctcaacga ccggatctgc
     6661 cgctggccgg tgggccaccc cggcgagccc gatttccact tctgc
//
LOCUS       DASRYE010000100         9550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_200370,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000100 DASRYE010000000
VERSION     DASRYE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200370"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFF98_05540"
     CDS             complement(<1..869)
                     /locus_tag="VFF98_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU63125.1"
                     /translation="MAARALPRGSVPLALAVAVSVALLFAFAPARRARPAAAGLALSA
                     PLPASVPPGVVLRIGDPVDSWIFQHLGWEKQLPFRIQWAEITGGPGVTEAFNAHALDV
                     GFGANVPPVHARWSGIPVRIIGFRQFAPGHAPLYVFGLAPGAGIRTIADLKGKRIAFS
                     RSQVQAQVVLETLAAAGIDRRDVSLVELPSSIGGDVYTNALASREVDAAPLGGGVIAY
                     HYLTKFADRGAAVLAHPPFPDDGLTLYTPESVLEDPGKAAALSVFVRYWGKAQAWANA
                     HPEELARGYYMGVR"
     gene            complement(881..1783)
                     /locus_tag="VFF98_05545"
     CDS             complement(881..1783)
                     /locus_tag="VFF98_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU63126.1"
                     /translation="MSLSGLYGRSDDAESLATLQRALDLGITFFDTATGYGQGHNERL
                     FGRALGARRKDIVLASKFLHRQGERRIDAREAVEASLARLGVESIDIYYLHRVDSEIP
                     VEESIGQLGRLVEEGKIGGVGISEASADQLRRGHATYPITALQSEYSLWSRDVETEIL
                     PTARALGIGFVGFSPLGRGFLAGALPSDADDRRNIHPRYREEAIAANARRRRTIEEVA
                     QRLGATPAQVCIAWVQAKGVVPIPGTRHIRHLEDNWAANDLALDAPTIAELDQAFPHG
                     TTAGDRFPPDQMKHVPVPPAPVSA"
     gene            1990..4311
                     /locus_tag="VFF98_05550"
     CDS             1990..4311
                     /locus_tag="VFF98_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63127.1"
                     /translation="MHSTVRSGFSAIALAGALAALPAQATEAGATTAEATTAEAAPAP
                     GDDGAEIKVTARHQVERAQDIPLSLSVVSGAGLERTGGYTIADLQQRTPSLTAYNSNP
                     RNSSVGMRGIGVSSASDGLDSSVGVYVDNVYFGRPGMALQDLIDVDRVEVLRGPQGTL
                     FGRNSSAGVLNITTRAPAFQWGATAEVSGGNYGYHQERFSLTGPIVKDLLAFRVTGFD
                     TERDGVTPNLATGRAANSINRQGGRAQLLLTPGSRLKIRLIGDYSRERDSCCVSVIRN
                     VLPASTSSGTSRTLQALAALGWTPTASSTSVAWDAPQSMRTDQWGTSLQADWDLGFAT
                     ATSVTAYRYWHFDAYQESDYTPFDILDRNIAITRDRQVSQELRLASDPARRLSWQVGA
                     YYFHQQLRDHYILNQFGSAASAFFTAYARTSNPSAAAVVVAPGSQYLDDVYTTVDSFA
                     TFGQANFRLTPRLTITGGLRYTHDQRDGISASSTTGTPYASTSIPFNYNVSVKGNNVS
                     WLASLSWKPAGDILAYASASTGYKGAGLNLNSSTSTGTPLILQPEKVHNYEAGVKQQL
                     FGHRVTLNIDGYWTLLNGLQANIYPTNGGKSYLANVGNVRARGIEVDGDWKVLPGLTL
                     SFNGSFNDTRYSSYHNAPCPVGGASVCDLTGRRVYQSPKWVGNAILDYRFDAGHEVTP
                     YFVARYAYRSAMYGTVDDSAYGIVPGYGLASFRVGASFARRYDASLWVDNAFDKTYYE
                     NMNALAVVGAGTYGYGGKLGAPRTFGGTIRATF"
     gene            4346..5428
                     /locus_tag="VFF98_05555"
     CDS             4346..5428
                     /locus_tag="VFF98_05555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU63128.1"
                     /translation="MSQTSEFLWYIPNVVQGGHRGDPVFHGHNSLETLTSHALALEQH
                     GWGGALIGTGWGRPDTFTLATAITARTTRFEPLVAIRPGYWQPAHLASAAATLDHLGG
                     GRLRINIVSGRDDLAAYGDSEGNQAERYGRTKEFMRLVRRLWTEDAVDFAGAHFQVHQ
                     STVEPRIAVRGDRRHPRLYFGGASAAAEQVAATEADVQLFWGEPLADVAERIARLKAL
                     SRDLDRDLAPLQFGLRITTLIRDTSAQAWADAEAKVAALKAEHAQPERHQPDEHRRAA
                     AVGQQRLLDLQARGEVLDDNLYTTPGRYGAGGAATTWLVGSAEEVAASLRKYRDLGIT
                     HFVLSDTPYLQEIAHQGDQLLPLLRD"
     gene            complement(5401..6828)
                     /locus_tag="VFF98_05560"
     CDS             complement(5401..6828)
                     /locus_tag="VFF98_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU63129.1"
                     /translation="MRLRPILAMALPVALAGCNLAPAYHPPAIAAPAQWRPQPGWAPA
                     SPQDSLPRGAWWAVFGDPVLDDLEGRLDGANPDLQAALARYDGAAAYAQQARGALLPG
                     VDGQGSATRNRQSDERPLRGSGQPDLYTADQANGIASYEPDLWGRLRNELAARRDLAQ
                     ASDADRAALRLSLEAQLASAYFALRGLDADLELLAGTVAAYRQSLTLTQTLYSGKIAA
                     QMDVSRAQVQWENARAMEADLAASRDRMADAIAVLAGAAPSTFTLAAAPHLAGLPAIP
                     AGLPATLLERRPDIAAAERAAAAANARIGVARAAFYPAISFDAAGGLMSQGTDPFKAG
                     DLFWSLGPSISLPLFTGGRLQGQLAGARAQWAEAAAHYRSVALNAFREVEDQRALMAR
                     LAQEEGFSASASTAARQTVAATTQLYAQGATSYLEVVTAQTALLNAQRAALDIRTRRL
                     VASVGLIRALGGGWSAAQSRSSGSS"
     gene            complement(6825..7910)
                     /locus_tag="VFF98_05565"
     CDS             complement(6825..7910)
                     /locus_tag="VFF98_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63130.1"
                     /translation="MAAIAVLVAAGGLASRAATARRNAAWAQAAAIPTVALARMETGP
                     QTAITLPGTVQPFRKAQIYARVNGYLKAWTADIGSDVRAGQTLALVDTPDLDQQRAQA
                     RGDLAMAQANARLADLTASRWHALQASGAVSQQVIDEKLGDAQAKRAVVQAAQARLRG
                     IEALVAYKRIASPFDGIVTARKTDIGALINAGASGQELFEVADLSRLRIYVQVPQTLA
                     ATLKVGDSAQITLPEQPGTTHPATIAAISHALDAATRTMLIQLQAPNPGGRIGAGAYC
                     QVLFAAAASGSAPRLPATAIIATNTGAEVAVLGPGNVVAIRPVRLGHDDGHAVEVVAG
                     LAPGERVIDSPPETLRNGDHVRLGNGA"
     gene            complement(7997..>9550)
                     /locus_tag="VFF98_05570"
     CDS             complement(7997..>9550)
                     /locus_tag="VFF98_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63131.1"
                     /translation="LGFLGCSALSLLLVPLLGQDFFPPLDGGQIRLHMRAPTGTRIEE
                     TTRIADSVGTAIRQILPPGEVAGVVANIGLSVSGINMAYDNAGTIGTGDATIQVALSP
                     DHGATAGHVQRLREELPRRFPGTSFDFLPADMVSQILNFGSPAPIDLQIVGADTAADR
                     AFANAMLARIRQIPGITDARIQQAFAAPQLDVDINRAMAGMVGLTEKDAANAMLTTYA
                     GSTQVAPTYWLNPKTGVSYPVSIQTPQRDVASLEDLNNTAISASGSAAPQLLRNLGSI
                     RRAPADAVVSHYNVRTVIDIYASPQNRDLGAVARDVQQVAADMRASLPKGATLALNGQ
                     IATMNSAYSQLYMGLAAAIVLIFLLIVVNFQSWLDPLVIVLGLPTALAGIAWMLFACE
                     TPLSVPALTGAIMCMGVATANSILVIAFARERLAAGASALEAARAAGAARFRPVLMTA
                     TAMVLGMVPMAIEPGQNMPLGRAVIGGLVFATCATLVLVPALFALMHGRAPRHPPAHR
                     QDACDTPER"
BASE COUNT         1487 a         3275 c         3302 g         1486 t
ORIGIN      
        1 cgcacgccca tgtaatagcc gcgcgccagc tcctcggggt gggcattggc ccaggcctgc
       61 gccttgcccc aatagcgcac gaaaacgctg agcgcggccg ccttgccggg atcttccagc
      121 acgctttcgg gggtgtagag cgtcagcccg tcatccggga aggggggatg ggccagtacc
      181 gccgcgccgc gatcggcgaa tttggtgaga tagtgatagg cgatcacgcc gccgcccaga
      241 ggcgctgcat ccacctcgcg gctggccagc gcattggtgt acacatcgcc gccgatgctg
      301 gagggcagct cgaccaggct gacatcgcgc cggtctatgc cggcggccgc cagcgtttcc
      361 agcaccacct gcgcctgcac ctggctgcgg ctgaaggcga tgcgcttgcc cttcaaatcg
      421 gcgatggtgc ggatgccggc gccgggcgcc aagccaaaca cataaagcgg cgcatggccc
      481 ggcgcaaatt gccgaaagcc gatgatgcgc acgggaatgc cgctccaccg ggcgtgcacc
      541 ggcggcacat tggcgccaaa gcccacgtcc agcgcatggg cgttgaaagc ctcggtgacg
      601 cccggcccgc cggtgatctc ggcccactgg atgcggaagg gaagctgctt ttcccagccc
      661 agatgctgga agatccagct gtccaccgga tcgccgatgc gcagcaccac gcccggcggc
      721 acgctggccg gcagcggcgc cgacagcgcc agccctgccg ccgccggcct ggcccgccgc
      781 gccggggcaa aggcgaacag cagcgcgacg gacacggcga cggccagcgc caggggcacc
      841 gatccgcgcg gcagggctct ggcggccatg gccgggcccg tcaggccgag acgggcgccg
      901 gcggtaccgg cacatgcttc atctggtcgg gcgggaagcg gtcgcctgcg gtggtgccgt
      961 gggggaaggc ctggtcaagc tcggcaatgg tcggcgcatc cagcgccaga tcattggcgg
     1021 cccaattgtc ttccagatgg cggatgtgac gcgtgccggg gatcggcacc acgcccttgg
     1081 cctgcaccca ggcgatgcac acctgcgccg gcgttgcccc cagcctttgc gccacttcct
     1141 cgatggtgcg ccggcgccgg gcattggcgg cgatggcttc ctcgcggtag cgcgggtgga
     1201 tgttgcggcg gtcgtcggca tcgctgggca gggcgccggc cagaaagccc cggcccagcg
     1261 gcgaaaagcc cacaaagccg atgcccaagg cgcgcgcggt gggcaggatc tcggtttcca
     1321 catcgcggga ccacagcgaa tattcgcttt gcagcgcggt gatcggatag gtggcgtggc
     1381 cgcggcgcag ctggtcggcg ctggcctcgg aaatgccgac gccgccgatc ttgccttcct
     1441 ccaccagccg gcccagctgg ccgatcgatt cctccaccgg gatctcgctg tccacccggt
     1501 gcaggtagta gatgtcgatg ctctcgacgc ccagccgcgc gaggctggcc tccacggcct
     1561 cgcgcgcgtc gatgcggcgc tcgccctggc ggtggaggaa cttgctggcc agcacgatat
     1621 ccttgcgccg ggcgccaagc gcgcggccga acaggcgctc gttgtggccc tggccgtagc
     1681 cggtggcggt gtcgaagaag gtgatgccca gatccagcgc gcgctgcagg gtggcgagcg
     1741 attctgcatc gtccgagcgg ccatagaggc ccgaaaggct catccccccg agcccctgct
     1801 tgctgacttc gaatgctccg atgcggggca tgacttctct cccaactggc tgaatgatgg
     1861 cgcctctttc gggcaggccg gaggcgggtg ccacatgcga aaactttagc ggcgcttata
     1921 atatctatct tccagatagg aattatcaat ttaagcgagg gggccaagcc atccaggggg
     1981 cgcagccaca tgcacagcac agtaagatcc ggattttcgg ccattgctct ggccggggcg
     2041 ctggccgcgc tgccggcaca ggccaccgaa gccggcgcca ccactgccga agccaccacc
     2101 gccgaagccg cgcccgcccc gggcgatgac ggcgcggaaa tcaaggtcac cgcgcgccat
     2161 caggtggagc gcgcccagga tattccgctt tcgctgtcgg tcgtctcggg cgccgggctg
     2221 gagcgcaccg gcggctacac catcgccgat ctccagcagc gcacgcccag cctcaccgcc
     2281 tacaacagca atccgcgcaa ttcctcggtg ggcatgcgcg gcatcggcgt ttccagcgcg
     2341 tccgacgggc tggattcctc ggtcggcgtc tatgtcgaca atgtctattt cggccggccg
     2401 ggcatggccc tgcaggatct gatcgatgtg gaccgggtgg aagtgctgcg cgggccccag
     2461 ggcacattgt ttggccgcaa cagttcggcc ggcgtgctca acatcaccac ccgcgcgccg
     2521 gccttccagt ggggcgccac ggcggaagtg tcgggtggca attatggcta tcatcaggag
     2581 cgcttcagcc tcaccgggcc gatcgtgaaa gacctgctgg ccttccgcgt caccggtttc
     2641 gacaccgagc gcgacggcgt gacgcccaac cttgccaccg gccgcgcggc caacagcatc
     2701 aaccgccagg gcggccgcgc gcaactgctg ctgacgccgg gcagcaggct gaagatccgc
     2761 ctcatcggcg attactcgcg cgagcgggac agctgctgcg tcagcgtgat ccgcaacgtg
     2821 ctgccggcca gcacttccag cggcaccagc cgcaccctgc aggcgctggc cgcccttggc
     2881 tggaccccca ccgccagcag caccagcgtt gcctgggacg cgccgcaatc catgcgcacc
     2941 gaccagtggg gcacatcgct gcaggccgat tgggatctgg gctttgccac ggccacctcg
     3001 gtcacggcct atcggtattg gcatttcgac gcctatcagg aatccgatta cacgccgttc
     3061 gacatccttg atcgcaatat cgccatcacc cgcgaccggc aggtgtcgca ggaattgcgg
     3121 ctggcttccg atccggcccg gcgcctgtca tggcaggtgg gggcctatta cttccaccag
     3181 caattgcgcg atcactatat cctcaaccag ttcggcagcg cggcctcggc cttcttcacc
     3241 gcctatgcgc gcaccagcaa cccgtctgcg gcagccgtgg tggtggcgcc gggctcgcaa
     3301 tatctcgatg atgtctacac aacggtcgac agtttcgcca ctttcggcca ggccaacttc
     3361 cgcctgacgc ccaggctgac gatcaccggc ggcctgcgct acacgcatga ccagcgcgat
     3421 ggcatctcgg ccagttccac caccggcacg ccttatgcct ccacctcgat cccgttcaat
     3481 tacaacgtct cggtgaaggg caacaatgtc tcgtggctgg ccagcctgtc gtggaagccg
     3541 gccggcgaca tccttgccta tgcctccgcc tcgaccggct acaagggcgc cggcctcaac
     3601 ctcaattcct ccaccagcac cggcacgccg ctgatcctgc agccggaaaa ggtgcataat
     3661 tacgaggccg gggtgaagca gcagctgttc ggccaccgtg tcacgctcaa catcgatggc
     3721 tactggacgt tgctcaatgg cctgcaggcc aacatctatc ccaccaacgg cggcaagtcc
     3781 tatctcgcca atgtcggcaa tgtgcgcgcc cgcggcatcg aggtggatgg cgactggaag
     3841 gtgctgccgg gcctcaccct ctcgttcaac ggctcgttca acgacacgcg ctacagcagc
     3901 tatcacaacg ccccctgccc ggtgggcggg gcatcggtgt gcgatctcac cgggcggcgg
     3961 gtctatcagt cgcccaagtg ggtgggcaat gccatcctcg attatcgctt cgatgccggc
     4021 cacgaggtga cgccctattt cgttgcccgc tatgcctatc gttccgccat gtatggcacg
     4081 gtggacgaca gcgcctatgg catcgtgccg ggctatgggc tggccagctt tcgcgtcggc
     4141 gccagctttg cccgccgcta cgacgcctcg ctgtgggtcg ataacgcttt cgacaagacc
     4201 tactacgaga acatgaacgc cctcgccgtc gtgggggccg gcacctacgg ttatggcggc
     4261 aagctgggcg cgccgcgcac ctttggcggc accatccgcg ccactttctg atcctccccc
     4321 catctcctgc cagacgagaa tgcacatgag ccagacaagc gaattcctgt ggtatatccc
     4381 caatgtggtg caaggcgggc atcgcggcga tccggtgttt catggccaca acagcctcga
     4441 gacgctgacc agccacgcgc tggcgctgga gcagcacggt tgggggggcg cgctgatcgg
     4501 caccggctgg gggcggcccg atacgttcac gctggccact gcgatcactg cgcgcaccac
     4561 ccgtttcgag ccgttggtgg ccattcgccc gggctattgg cagccggcgc atctggcctc
     4621 tgcggcggcc acgctggatc atctgggcgg cgggcggctg cgcatcaaca tcgtctcggg
     4681 ccgcgacgac ctggccgcct atggcgacag cgagggcaac caggccgagc gctatggccg
     4741 cacgaaggag ttcatgcggc tggtgcgccg gctgtggacg gaagacgcgg tggattttgc
     4801 cggcgcccat tttcaggtgc accaatccac cgtggagccg cgcattgccg tgcggggcga
     4861 caggcggcat ccgcggctct atttcggcgg cgcttcggcc gcggccgagc aggtggcggc
     4921 caccgaggcc gatgtgcagc tgttctgggg cgagccgctg gccgatgttg ccgaacgcat
     4981 cgcccggctg aaggcgctga gccgggatct cgaccgggac cttgctccgc tgcagtttgg
     5041 cctgcgcatc accacgctga tccgcgatac cagcgcgcag gcctgggccg atgccgaggc
     5101 caaggtggca gcgctgaagg ccgagcacgc ccagcccgaa cgccaccagc ccgacgagca
     5161 ccgccgcgcc gccgctgtcg gccagcagcg gctgctggat ctgcaggcgc gcggcgaagt
     5221 gctggacgac aacctctaca ccaccccggg ccgctatggc gcgggcgggg cggccaccac
     5281 ctggctggtc ggctcagctg aggaagtggc ggcctccttg cgcaaatacc gcgatctggg
     5341 gatcacccat ttcgtcctgt cggacacgcc ctacctgcag gaaatcgccc atcagggcga
     5401 tcagctgctg ccgctgctgc gcgactgagc cgcgctccag ccgccgccca gcgcgcggat
     5461 cagccccacg ctggccacca gccggcgggt gcggatatcc agagcggcgc gctgggcgtt
     5521 tagcagggcg gtctgggcgg tgacgacttc gaggtagctg gtggcgccct gggcataaag
     5581 ctgggtggtg gcggccaccg tctgccgggc ggcggtgctg gcggaggcgg aaaagccctc
     5641 ctcctgcgcc agccttgcca tcagcgcgcg ctggtcctcc acctcgcgaa aggcgttgag
     5701 cgccacgctg cggtaatggg cggcggcctc ggcccattgc gcgcgggcgc ccgccagctg
     5761 cccttgcagc cgcccgccgg tgaacagcgg caggctgatc gagggcccca gcgaccagaa
     5821 cagatcgccc gccttgaagg gatcggtgcc ctggctcatc aacccgcctg cggcatcgaa
     5881 gctgatcgcc ggatagaaag cggcccgcgc cacgccgatg cgggcattgg cggcggccgc
     5941 ggcgcgctcg gccgcggcga tatcgggccg gcgttcgagc agggtggcgg gcaggccggc
     6001 gggaatggcg ggcaggccgg ccagatgcgg cgctgctgcc agcgtgaagg tggaaggggc
     6061 agcgcctgcc agcacggcga tggcatcggc catccggtcg cggctggcgg ccagatcggc
     6121 ctccatggcc cgggcattct cccactgcac ctgcgcgcgc gagacatcca tctgcgcggc
     6181 gatcttgccg ctgtagagcg tctgcgtcag cgtgaggctc tggcgatagg cggccaccgt
     6241 gccggccagc agctccagat cggcatcgag gccgcgcagc gcgaaatagg cgctggccag
     6301 ctgcgcttcc aggctgaggc gcagcgcggc gcgatcggca tcgcttgcct gtgccagatc
     6361 gcggcgggcg gccagttcgt tgcgcagcct gccccacagg tccggctcgt aggaggcgat
     6421 gccgttggcc tgatcggcgg tatacaggtc gggctggccg ctgccgcgca ggggccgctc
     6481 gtcagactgg cggttgcgcg tggcgctgcc ttgcccatcc acgccgggca gcagcgcgcc
     6541 cctggcctgc tgggcatagg cggccgcgcc atcgtagcgg gcaagggcgg cctgcagatc
     6601 ggggttggcg ccatccagcc ggccttccag atcatccagc accggatcgc cgaacaccgc
     6661 ccaccaggcg ccgcgtggca ggctgtcttg cgggctggcc ggggcccagc cgggctgggg
     6721 gcgccactgt gcgggcgcgg cgatggccgg cggatgatag gccggggcca ggttgcaacc
     6781 cgccaaagcc acaggcagcg ccatcgccag gatgggcctc agcctcacgc cccgttcccc
     6841 agccgcacat gatcgccgtt gcgcagcgtt tcgggcgggc tgtcgatcac gcgctcgccg
     6901 ggcgccaggc cggccaccac ttccaccgca tggccatcgt cgtggcccag cctgaccggc
     6961 cggatcgcca ccacattgcc cggccccagc accgccacct cggcgccggt gttggtggcg
     7021 atgatggccg tggccggcag gcgcggcgcc gaaccgcttg ctgctgcggc aaacagcacc
     7081 tggcaatagg cgcccgcgcc aatccggccg cccgggttgg gcgcctgcag ctggatcagc
     7141 atggtgcgcg tggccgcatc cagcgcgtgg gaaatggcag cgatggtggc agggtgggtg
     7201 gtgccgggct gctcgggcag ggtgatttgc gcgctatcgc ccactttcag cgtggcggcc
     7261 agcgtttgcg gcacctgcac atagatgcgc aggcggctga gatcggcaac ctcgaacaat
     7321 tcctgcccgc tggcgccggc gttgatcagc gcgccgatat cggtcttgcg cgcggtgacg
     7381 atgccatcga aggggctggc gatgcgcttg taggccacca gcgcctcgat cccgcgcagc
     7441 cgggcctggg ctgcctgcac caccgcgcgc ttggcctggg catcgcccag cttctcgtcg
     7501 atcacctgct gcgagacggc gcccgaggct tgcagcgcgt gccagcggct ggcggtaaga
     7561 tcggccagcc gggcattggc ctgcgccatg gcgagatcgc cccgcgcctg tgcccgctgc
     7621 tggtcgaggt cgggcgtgtc gaccagcgcc agcgtttgcc cggcgcgtac atcgctgccg
     7681 atatcggccg tccacgcctt gagatagccg ttcacccgcg catagatctg cgccttgcgg
     7741 aaaggctgca cggtgcccgg cagggtgatg gcggtttgcg gcccggtttc catccgtgcc
     7801 agcgcaacgg tggggatggc cgcggcctgg gcccaggcgg cattgcgccg cgccgtggcc
     7861 gcgcgcgagg caaggccgcc tgccgccacc agcacggcga tggccgccac gccggatccg
     7921 gcggcgatga gggcccgctt gccgggccct gtgcgaggca gtgtcgttgt aggatgcggt
     7981 gtcgtcatcg tggccctcat ctctcaggcg tgtcgcaggc gtcttggcgg tgggcgggcg
     8041 gatggcgcgg cgcgcggcca tgcatcagcg cgaaaagcgc cggcaccagc accagcgtgg
     8101 cgcaggtggc aaacaccagc ccaccgatca ccgcccggcc cagcggcatg ttctgccccg
     8161 gctcgatcgc catcggcacc atgcccagca ccatggcggt ggcggtcatc agcaccgggc
     8221 ggaagcgggc ggcgccggcc gcgcgggcgg cctccagcgc gctggccccg gccgccagcc
     8281 gttcacgcgc gaaggcgatg acgagaatgg aattggcggt tgccacgccc atgcacatga
     8341 tcgccccggt gagcgccggc acggacagcg gcgtttcgca ggcaaacagc atccaggcga
     8401 tgcccgccag cgccgtgggc aggcccagca cgatcaccag cggatcgagc caggactgga
     8461 agttcaccac gatcagcagg aagatcagca cgatggcggc ggcaaggccc atatagagct
     8521 ggctgtaggc gctgttcatc gtggcgatct ggccgttgag ggcaagggtg gcgcccttgg
     8581 gcaggctggc gcgcatatcg gccgccacct gctgcacatc gcgggccaca gcgcccagat
     8641 cgcggttttg cgggctggcg taaatgtcga tcaccgtgcg cacattgtag tgcgagacca
     8701 cggcatcggc gggcgcgcgg cggatgctgc ccagattgcg cagcagttgc ggcgcggcgc
     8761 tgccggaagc cgatatggcg gtgttgttca gatcctccag gctcgccaca tcgcgctggg
     8821 gtgtctggat cgagacggga taggagacgc ccgttttggg gttcagccaa taggtgggcg
     8881 ccacctgcgt gctgccggca taggtggtga gcatggcatt ggcggcatcc ttttcggtaa
     8941 ggccgaccat gcccgccatc gcccggttga tatcgacatc gagctggggc gcggcaaagg
     9001 cctgctgtat gcgcgcatcg gtaatgcccg ggatctgcct gatgcgcgcc agcatggcat
     9061 tggcaaaggc ccgatcggca gcggtatcgg cccccacgat ctgcaggtcg atcggcgcgg
     9121 gcgagccgaa attgaggatc tggctgacca tgtcggccgg caggaaatcg aagctggtgc
     9181 cggggaagcg gcgcggcagt tcctcgcgca ggcgctgcac atggccggcg gtggcgccat
     9241 gatcgggcga gagggcaacc tggatcgtcg catccccggt gccgatagtg ccggcgttgt
     9301 cgtaggccat gttgatgccg ctgacgctga ggccgatgtt ggccaccacg cccgccactt
     9361 cgcccggcgg caggatctgg cggatggcgg tgcccacgct gtcggcgatg cgggtggtct
     9421 cctcgatgcg ggtgccggtg ggcgcgcgca tgtggaggcg gatctggccg ccatcgagcg
     9481 gcgggaagaa atcctgcccc agcagcggaa ccagcagcag cgacagcgcc gaacagccaa
     9541 ggaagccgag
//
LOCUS       DASRYE010000101         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_6015,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000101 DASRYE010000000
VERSION     DASRYE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6015"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFF98_05575"
     CDS             complement(<1..527)
                     /locus_tag="VFF98_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU63132.1"
                     /translation="MLPAAPLPPAVPAVWQGDVVILGAGAAGLMCAGQAGQAGHRVLV
                     VDHAAQPGAKILISGGGRCNFTNLHTAPDRYLSQNPHFARSALSRYGPRDFLALVESH
                     GIAWHEKTLGQLFCDSSARQILAMLLEECAKGQVEIALGAPLGEVGHADGRFHLTFGG
                     RRAEAPRLVIATGGPS"
     gene            608..781
                     /locus_tag="VFF98_05580"
     CDS             608..781
                     /locus_tag="VFF98_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63133.1"
                     /translation="MRTRLSICQRKRRYSTQAEAIAAALAAPFPLRPYRCDRCLGYHL
                     TGRLKGKRRLPGG"
     gene            complement(789..1856)
                     /locus_tag="VFF98_05585"
     CDS             complement(789..1856)
                     /locus_tag="VFF98_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZU63134.1"
                     /translation="MDLSARLGLDIPLFQAPMAGVATPALAACVSNAGGLGALGLGAS
                     DAAAARAMITELRARTARPFGVNLFTHASPQADPVREAAWINALAPLFARFGAAPPAQ
                     LHSPYRSLADDPEMVAMLLAEKPAVISFHFGLPPAEVLAALRTTGALLMASATSLAEA
                     RAIAAAGLDAVIAQGIEAGGHRGIFEPAGADDALGTFALTRLLVREGALPVVAAGGIM
                     DGAGIAAALALGAEAAQLGTAFIACPESAADAAYRAALAGPGAQHTRLTAAISGRPAR
                     ALGNRFTALAEELGTLAIPAYPIAYHAGKALNAAAKAAGEDGYGAQWAGQGAPLARPL
                     PTAELVALLRAELAAAQAGPR"
     gene            2153..2242
                     /gene="kdpF"
                     /locus_tag="VFF98_05590"
     CDS             2153..2242
                     /gene="kdpF"
                     /locus_tag="VFF98_05590"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZU63135.1"
                     /translation="MNLPLILAGATALGLVAYLLAVLMRPEKF"
     gene            2256..>3697
                     /gene="kdpA"
                     /locus_tag="VFF98_05595"
     CDS             2256..>3697
                     /gene="kdpA"
                     /locus_tag="VFF98_05595"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZU63136.1"
                     /translation="MTFSGWALILVFVAAIVALARPLGLWLFALYEGRRTPLHRLLGP
                     VERGFYRLAGINPDAQQGWRGYALHMLMFQVVTVLATYALLRAQAVLPLNPRGLAGLP
                     VDLSMNTAISFATNTNWQAYAGESRLSNLSQMLALAPHNFLSAATGIAVAFALFRGFA
                     RAGGSGSGAKPAGLGNFWADITRITLYLLLPLCAFYALVMVACGVPQTLASTIDLTTL
                     EGAHQTLVVGPVASQEAIKMLGTNGGGFFNANSAHPFENPTAFVNFLQMLSIFAIGVG
                     LTYCFGKAVGDVRQGRAILAAMMILFLIGVGITYWQEAGGNPILHHAGLAGPNLEGKE
                     TRFGVAASALFAVVTTAASCGAVNAMHDSLMPLGGLVPMFNMELGEIVVGGVGSGIYG
                     FLLFALLAVFIAGLMVGRTPEYVGKKIESREVKLAVLAIVVLPLAILGGTALASVLAA
                     GLAGPAVKGPHGFSEILYAFTSATANNGSA"
BASE COUNT          516 a         1265 c         1297 g          619 t
ORIGIN      
        1 gagggcccgc cggtggcgat caccagcctg ggcgcttcgg cccgccgccc gccgaaggtg
       61 aggtgaaagc gcccgtcggc atggccaacc tcgcccagcg gcgcgcccag cgcgatttcc
      121 acctgcccct tggcgcattc ctccagcagc atcgccagga tctgccgggc cgaactatcg
      181 cagaacagct ggcccagcgt cttctcgtgc caggcgatgc cgtggctttc caccagcgcc
      241 agaaaatcgc gcgggccata gcggctgagc gccgagcggg caaaatgcgg gttttgcgag
      301 agataacggt cgggcgcggt gtgcaggttg gtgaaattgc agcgcccgcc gccggaaatg
      361 aggatcttgg cgccgggctg ggcggcatgg tccaccacca gcacgcggtg gccggcctgc
      421 ccggcttggc ctgcgcacat caacccggcc gcgcccgcgc ccagaatgac gacatcgccc
      481 tgccagacgg cgggaacagc gggcggcaaa ggggcggcag gcaacatggc cgcggcccat
      541 agccgacagc gcgcccgccg gccagagtgc ttgcctgcgc gccgatccgc ggctagcccg
      601 gcccatcatg cgcacccggc tcagcatctg ccagcgcaag cggcgctatt ccacccaggc
      661 cgaggcgatt gccgcggcgc tggcggcgcc tttccccttg cgcccctatc gctgcgacag
      721 gtgccttggc tatcacctca ccgggcggct gaagggcaag cgccgcctgc cgggcggctg
      781 agccagcctc agcgcgggcc tgcctgcgcc gctgccagtt cggcgcgcag cagggccacc
      841 agctcagctg tcggcagcgg gcgggccagc ggcgcgcctt gcccggccca ttgcgcgcca
      901 tagccatcct cgccggcggc cttggcggcc gcgttcagcg ccttgccggc gtgataggcg
      961 ataggataag cggggatggc cagcgttccc agttcctcgg ccagcgccgt gaagcggttg
     1021 cccagcgcgc gcgccggccg gcccgagatc gccgctgtca gccgcgtgtg ctgcgcgccg
     1081 ggcccggcca gcgccgcgcg ataggccgca tcggctgcgc tttccgggca ggcgatgaag
     1141 gcggtgccca gctgggcggc ctctgccccc agcgccagcg cggcggcgat gcccgcccca
     1201 tccatgatgc cgccggccgc caccacgggc agcgccccct cgcgcaccag caggcgggtg
     1261 agggcgaagg tgcccagcgc atcgtctgcg cctgcgggct cgaagatgcc gcgatggccg
     1321 cccgcctcga tcccttgcgc gatcaccgca tcaaggccgg cagcggcaat cgcccgcgcc
     1381 tcggccagcg aggtggcgct ggccatcagc agggcgccgg tggtgcgcag cgcagccagc
     1441 acctctgccg gcggcaggcc gaaatggaag ctgatgacgg ccggcttttc agccagcagc
     1501 atggccacca tctccgggtc gtcggccaag ctgcgatagg ggctgtgcaa ctgcgcgggc
     1561 ggggccgcgc caaagcgagc gaacagcggc gccagcgcgt tgatccacgc cgcctcgcgc
     1621 acaggatcgg cttgcgggct ggcatgggtg aagaggttga cgccgaaggg ccgcgcggtg
     1681 cgggcgcgca gttcggtgat catcgcccgg gcagcggcgg catcgctggc gcccagcccc
     1741 agcgcgccca gcccgcccgc gtttgacacg caggccgcca gcgcgggcgt ggccacaccg
     1801 gccatcggcg cctgaaacag cgggatatca aggcccagcc ttgcggaaag atccatctct
     1861 tgcggctcct caaccatccc gtccgccgcc ttgcataggc cggcggggcc ggcgcgccaa
     1921 gagtctctgg cctttatgct ttctttatgc ggcgggccgg ctttgccgtg gatattttac
     1981 gccgcacagg cgcattgggc aggcggacca accggccttt gccccatgcg gcaagggccc
     2041 catccgcaag gtgaatggca tgaccgatct tctctggctg ggcttgctgg cggcgctggg
     2101 cggcgccagc ctggtgttcc tgcgcctgtg cgatgcggcc tgagggcgct gcatgaacct
     2161 ccccctcatc ctggccggcg ccactgcgct gggccttgtc gcctatctgc tggccgtgct
     2221 gatgcggccg gaaaaattct gaaaggaagc cgccgatgac cttctccggc tgggcgctga
     2281 tccttgtctt cgtggctgcc atcgtggcgt tggccaggcc tttgggcctg tggctgtttg
     2341 cgctttatga aggccgccgc acgccgctgc accgcctgct gggcccggtg gagcgtggct
     2401 tctaccggct ggccggcatc aaccccgatg cgcagcaggg ctggcgcggt tatgcgttgc
     2461 acatgctgat gtttcaggtg gtaacggtgt tggccaccta tgcgctgctg cgcgcgcagg
     2521 cggtgctgcc gctcaatccg cgcgggctgg cggggctgcc ggttgatctg tcgatgaaca
     2581 cggcgatctc ctttgccacc aacaccaact ggcaggccta tgccggcgag agcaggctgt
     2641 cgaacctgtc gcagatgctg gcgctggcgc cgcataattt cctttcggcc gccaccggca
     2701 ttgcggtggc ctttgcgctg ttccgcggct ttgcgcgggc cggcgggagc gggagcgggg
     2761 ccaagcctgc ggggctgggc aatttctggg ccgatatcac ccgcatcacg ctgtatctgc
     2821 tgctgccgct gtgcgccttc tatgcgctgg tgatggtggc ctgcggcgtg ccgcagacgc
     2881 tggccagcac gatcgatctc accacgctgg aaggcgcgca tcaaacgctg gtggtgggcc
     2941 cggtggccag ccaggaagcg atcaagatgc tgggcactaa tggcggcggc tttttcaacg
     3001 ccaattcggc ccatccgttc gagaacccca ccgcttttgt caatttcctg cagatgctgt
     3061 cgatcttcgc cattggcgtg gggctcacct attgctttgg caaggcggtg ggcgatgtgc
     3121 ggcagggccg ggccattctg gccgcgatga tgatcctttt cctcatcggc gttggcatca
     3181 cctattggca ggaggcgggc ggcaacccca tcctgcacca cgcggggctg gccgggccca
     3241 atctggaagg caaggaaacc cgctttggcg tggccgcctc cgccctgttc gcggtggtga
     3301 caacggcggc cagctgcggc gcggtcaacg ccatgcatga cagcctgatg ccgctgggcg
     3361 gcctggtgcc catgttcaac atggaactgg gcgagatcgt cgtcggcggg gtgggctcgg
     3421 gcatttatgg cttcctgctg tttgccctgc tggcggtgtt cattgccggg ctgatggtgg
     3481 ggcgcacgcc cgaatatgtc ggcaagaaga tcgagagccg cgaggtgaag ctggcggtgc
     3541 tggcgatcgt ggtgctgccg ctggccattc tgggcggcac ggcgctggcc tcggtgctgg
     3601 cagcggggct ggcaggcccg gcggtgaagg gcccccatgg cttttccgag atcctttatg
     3661 ccttcacttc ggccacggcc aacaatggct cggcctt
//
LOCUS       DASRYE010000102         7620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_252573,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000102 DASRYE010000000
VERSION     DASRYE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252573"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFF98_05600"
     CDS             complement(<1..1096)
                     /locus_tag="VFF98_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU63137.1"
                     /translation="MRLISPFSARGRLLPVLLPALLAGCASVPHETPVLKPLDEAVLG
                     PAPTPTPAPAAPLAADWWHALGDPQLDRIMADAFAHSPTLADAEARIRAARAVLAMEK
                     GPLAPQITGSAGTTQERFSAKYIYPAPYGGGWFWDSEAAVSLNWSLDLAGRQKAMVAA
                     VRAAGDALVLDAAAARVSLGGAVAEAYVNLARAERQLAIAREFTASRTASLEVVRNRR
                     TNQLASDLDVASAETLLAEARQAEIRAAGNRALMVHALAALAGHGADYYATIGAPTLQ
                     LEGALPVPQLLPADLLGRRPDILAARANVAAARAGVRSAQAAFYPDVNLTAFVGASAL
                     GIGSLFTSQAITAGAGPALSLPIFEGGALKG"
     gene            complement(1227..2153)
                     /locus_tag="VFF98_05605"
     CDS             complement(1227..2153)
                     /locus_tag="VFF98_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63138.1"
                     /translation="MAHLPDLEAWAIFAKVAECGSFSRAGEELGLAKATVSKAITRLE
                     TRLSTSLFHRTTRKLSLTESGRLSLARASRILADGSAIEADVLEEAASPRGLVRIATI
                     NAVGAEVIAPVLPDFLRAYPDIELDLVLTENQVDVVAEGFDVAIVVDLGTTPTTARAT
                     HLSSLRRPLVAAPALIARHGLPTRPEDLARYPTVAPSHIPGSTEWHFQRAGTDPIAMR
                     IYPRFRANHADALLPALVGGVGIGLIAEMFIGHHLRDGRLVELLPEWASAPSPLYLIT
                     PPGRSRPARVRVLLDFLRHHIAQQDWLELSRP"
     gene            complement(2224..3102)
                     /locus_tag="VFF98_05610"
     CDS             complement(2224..3102)
                     /locus_tag="VFF98_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63139.1"
                     /translation="MSDRIVSWHGAPSKPAYTPPAGAIDAHCHVFGPMAQFPFSPKAK
                     YLPEDAGPDRLFALRDHLGFSRNVIVQASCHGTDNAATLNGIALSQGKARGVAVVDPA
                     ISDAEIAALHAGGMRGIRFNFLKRLVDNAPKDKFLEVAARIPQDWHIVIYFEADILEE
                     LRPFIAALPRLVVVDHMGRPDVTQGPDGADMKAFRALLDSREDIWFKPTCPDRLDLAG
                     GDPWNNFAAAVAPLVADYADRCIWGTDWPHPNMEKTIPDDGHIVDMIPRIAPTAELQQ
                     KLLVTNPMRLYWPEEL"
     gene            complement(3099..4160)
                     /locus_tag="VFF98_05615"
     CDS             complement(3099..4160)
                     /locus_tag="VFF98_05615"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HZU63140.1"
                     /translation="MTTSLSAPCMWMRGGTSKGGYFLAHDLPADTATRDAFLLGVMGS
                     PDPRQIDGLGGADPLTSKVAVVSSSTREGIDVDYLFLQVFVDQAIVTDAQNCGNILAG
                     VGPFAIERGLVAATGDETRVAIFMENTGQVAIATVQTPGGKVTYAGEAKIDGVPGAHA
                     PVPLEFRDTAGSSCGALLPSGNAVDVVNGVPVTLIDNGMPCVVMKAADVGITGYEDRA
                     WLDANTELKARIEAIRLAVGETMKLGDVREKSVPKMMLVAPPKNGGAVTVRSFIPHRA
                     HATIGVLGAVSVATACLLPGSPAAEVAEIPEGARKTLSVEHPTGEMTCVLETDESGAV
                     KSAALLRTARKLMDGVVFG"
     gene            complement(4242..4916)
                     /gene="ligK"
                     /locus_tag="VFF98_05620"
     CDS             complement(4242..4916)
                     /gene="ligK"
                     /locus_tag="VFF98_05620"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HZU63141.1"
                     /translation="MGGIVVQNIARADASVIDGLAACGVATVHEAQGRKGLLASYMRP
                     IYRGARIAGSAVTISAAPGDNWMVHVAIEQLQAGDILLLAPTSPCEDGYFGDLLATSA
                     QARGCRGLVIDAGVRDVRDLTEMHFPVWSKAIYAQGTVKNTIGSVNVPVICANQLVNA
                     GDVIVADDDGVVVVRREEAAEVLEKAQAREANEAAKREKLAAGVLGLDIYSMRERLEK
                     EGLKYV"
     gene            5107..6276
                     /locus_tag="VFF98_05625"
     CDS             5107..6276
                     /locus_tag="VFF98_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63142.1"
                     /translation="MDKKAAPERPLNIRHFAALAATVRHGSLTRAARSVNLTQPALTQ
                     AIAGLEASLGVTLFERGPQGMAATEPALLMAPRAEAAIAHVGSPRVTGTQLRALLALA
                     RAGSYAAAAEATGLSTASLHRAVADLQVALGQRLVDRRGHHVLLTAAGQRRARGFSLA
                     MAELRAGLAEVAAWQGKAAGRIVIGAMPLSRARWLPEAVNRFITGRPGVDIVVVEGSH
                     AELAGPLRDGEIDLMLGALRGEGETDGLVEEAVFEDQPVVVMRAGHPLLAQGEPYARL
                     LDYPWILPGSETPLRRHWEAMLRALGHVPPHVSVECGSTMALRQLLLTGEGLTLLSPE
                     QVAVELRAGLLATLAPPLRFARTIGITTRAGWRPTGAQAQMVALLRFCGAEVDGA"
     gene            6376..7188
                     /locus_tag="VFF98_05630"
     CDS             6376..7188
                     /locus_tag="VFF98_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HZU63143.1"
                     /translation="MTGVIALVGYGEAGSTFARAGDWPAQTGVWDVQAERRAVAGADG
                     LRAASSAAEALAGADLVLSLVTADSALPAARDYAALLKPGAIWADGNSVAPETKRAAA
                     AAIEAAGGRYVDVAILAPVHPARLNVPLLLAGAAAGAAQALLADLGFAHIRIVGAEVG
                     RASAIKMIRSVMVKGTEALTAEMMLAAQAAGVVDEVLASLDASEKPRPWAEKSAYNIE
                     RMVTHGLRRAAEMDESAKTLEGLGVEPMMTRGTVLRQRDMAGRTDRLIEEKE"
     gene            7196..>7620
                     /locus_tag="VFF98_05635"
     CDS             7196..>7620
                     /locus_tag="VFF98_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63144.1"
                     /translation="MTMVIDCHGHYTVLPKAHDAWREEQKAAFKAGTAAPAYPAISDD
                     EIRETIEANQLRLIRERGADMTIFSPRASAMAPHVGDQSVAVKWAQVCNDLIGRVAAL
                     YPEVFAPVCMLPQSPEADLTSSIAELERCVTQMGFIGCNL"
BASE COUNT         1171 a         2535 c         2740 g         1174 t
ORIGIN      
        1 tgcctttcaa cgcgccgccc tcgaagatcg gcaggctgag cgcggggccg gcgccggccg
       61 tgatcgcctg gctggtgaac agcgagccga tgcccagcgc cgaggcgccg acaaaggcgg
      121 tgaggttcac atccggatag aaggccgcct gggccgagcg cacgcccgcg cgcgcggccg
      181 ccacattggc gcgggcggcc aggatgtcgg gccggcggcc cagcagatcg gccggcagca
      241 gctgcggcac cggcagggcg ccttccagct gcagggtggg cgcgccgatg gtggcgtaat
      301 aatcggcgcc atggccggca agggcggcca gcgcatgcac catcagcgcg cggttgcccg
      361 cggcgcggat ctcggcctgg cgggcttcgg ccagcagggt ttcggcgctg gcgacatcga
      421 ggtcgctggc cagctggttg gtgcggcggt tgcgcaccac ttccagcgag gcggtgcgcg
      481 aggcggtgaa ttcgcgggcg atggccagtt gccgctcggc gcgggcgagg ttcacatagg
      541 cttcggccac agcgccgccc agcgagacgc gcgcggccgc cgcatccagc accagcgcat
      601 cgcccgccgc gcgcacggcc gccaccatcg ccttctgccg cccggccaga tcgagcgacc
      661 agttgagcga gacggccgcc tcgctatccc agaaccagcc gccgccatag ggggcggggt
      721 agatgtattt ggccgagaag cgttcctgcg tggtgccggc gctgccggtg atctgcggcg
      781 ccagcgggcc cttttccatc gccagcacgg cgcgggcggc gcggatgcgg gcctcggcat
      841 cggccagggt ggggctgtgg gcgaaggcat cggccatgat ccggtcgagc tgaggatcgc
      901 ccagcgcgtg ccaccagtcg gccgccagcg gcgcggcggg ggcgggggtt ggggtggggg
      961 cagggcccag caccgcctcg tccagcggct tcagcaccgg cgtttcatgc ggcacgctgg
     1021 cgcagccggc cagcaatgcc ggcagcagca cgggcagcag ccggcccctg gcagaaaacg
     1081 gagaaataag gcgcatcaat tgtgtccgat caggcgagca ggcaataata accctgttta
     1141 ttattcttgc cgcctgtcca taagtggcgg tcgggtgcgt gattatcccc cattgcgcca
     1201 catttgtgtt tccattcgcg ctataatcag gggcgggaca gttccagcca gtcttgctgg
     1261 gcgatgtgat ggcgcagaaa atcgagcagc accctgaccc gcgccgggcg cgaacggccc
     1321 ggcggcgtga tgagatagag cgggctgggc gccgaggccc attccggcag cagttccacc
     1381 agccggccat cgcgcaggtg gtggccgatg aacatttcgg cgatgaggcc aatgcccacc
     1441 ccgcccacca gcgccggcag cagcgcatcg gcatggttgg cgcgaaagcg cggatagatg
     1501 cgcatggcga tcggatctgt gccggcgcgc tggaaatgcc attcggtgct gccggggatg
     1561 tggctggggg ccacggtggg atagcgggcc agatcctcgg gccgggtggg caggccgtgg
     1621 cgggcgatca gcgccggggc ggccaccagc ggccggcgca ggctggacag gtgcgtggcg
     1681 cgcgcggtgg tgggcgtggt gcccagatcg acgacgatgg cgacatcgaa gccttcggcc
     1741 accacatcca cctggttttc ggtaagcacc agatccagct cgatatcggg ataggcgcgc
     1801 aggaaatcgg gcagcaccgg ggcgatcacc tcggcgccca cggcgttgat cgtggcgatg
     1861 cgcacaaggc cgcgcgggct ggccgcctct tccagcacat cggcctcgat cgcgctgcca
     1921 tcggcgagga tgcggctggc gcgggccagg ctgaggcggc cgctttcggt gagcgagagc
     1981 ttgcgcgtgg tgcggtggaa caggctggtg gagaggcgcg tttccagccg ggtgatggcc
     2041 ttggaaacgg tggccttggc caggcccagt tcctcgccgg cgcggctgaa gctgccgcat
     2101 tcggccactt tggcgaagat cgcccaggct tcgaggtcgg gcagatgcgc catcggcgcc
     2161 aaggctagca gagaaggccg gcagatgcga gggggctgcc cccgcgcatc tgcctgctct
     2221 attttacagt tcttcgggcc agtagagccg catcgggttg gtgacgagca gcttctgctg
     2281 cagctcggcg gtgggcgcga tgcgcgggat catgtcgacg atatggccat catcggggat
     2341 ggtcttttcc atgttggggt gcggccaatc cgtgccccag atgcagcggt cggcataatc
     2401 ggccaccagc ggcgccacgg cggcggcgaa attgttccac ggatcgccgc cggccagatc
     2461 cagccgatcc gggcaggtgg gcttgaacca gatgtcctcg cggctgtcga gcagggcgcg
     2521 gaaggccttc atgtcggcgc catcggggcc ctgggtcaca tcggggcggc ccatgtggtc
     2581 caccaccacc agccgcggca gggcggcgat gaaggggcgc agctcctcca gaatgtcggc
     2641 ctcgaaatag atgacgatgt gccagtcttg cgggatgcgg gcggccactt ccaggaactt
     2701 gtccttgggg gcattgtcca ccagccgctt caggaagttg aagcgaatac cgcgcatgcc
     2761 gccggcgtgc agcgcagcga tctcggcatc ggaaatggcc ggatccacca ccgccacgcc
     2821 gcgcgccttg ccctgcgaga gggcaatgcc gttcagcgtt gcggcattgt cggtgccatg
     2881 gcacgaggcc tgaacgatga cattgcgcga aaagcccaga tgatcgcgca gggcaaacag
     2941 cctgtctggc ccggcatcct cgggcagata tttggccttg gggctgaagg ggaactgcgc
     3001 catcgggcca aacacatggc aatgggcatc aatggcgccg gccggcggcg tgtaggccgg
     3061 cttcgacggg gcgccatgcc agctgacaat acggtcgctc atccgaacac aactccatcc
     3121 atcagtttgc gcgcggtgcg cagcagggcg gcgcttttga cagcgcccga ttcatcggtt
     3181 tccagcacgc aggtcatctc gccggtgggg tgctccaccg agagcgtctt gcgcgcgcct
     3241 tcggggatct cggccacttc ggccgcgggc gagcccggca gcaggcaggc cgtggccacc
     3301 gacaccgccc ccagcacgcc gatggtggca tgcgcgcggt gcggaatgaa gctgcgcacg
     3361 gtaaccgcgc cgccgttctt gggcggggcc accagcatca tcttgggcac ggatttctcg
     3421 cgcacatcgc ccagcttcat ggtttcgccc acggcgaggc gaatggcctc gatccgcgct
     3481 ttcagctcgg tgttggcatc cagccacgcg cggtcctcat agccggtgat gccgacatcc
     3541 gcggccttca tcaccacgca gggcatgcca ttgtcgatca gcgtgaccgg cacgccgttc
     3601 accacatcca ccgcattccc gctgggcagc agcgcgccgc aggaggagcc cgccgtgtcg
     3661 cgaaattcca gcggcaccgg ggcatgggcg cccggcacgc cgtcgatctt cgcctcgccc
     3721 gcgtaagtca ccttgccgcc cggcgtctgc accgtggcga tggccacttg cccggtattt
     3781 tccatgaaga tcgccacgcg ggtttcatcg ccggtggccg ccaccagccc gcgctcgatg
     3841 gcaaaggggc caacgccggc caggatgttg ccgcagttct gcgcatccgt cacgatcgcc
     3901 tgatcgacga agacctgcag gaacaggtag tccacatcga tgccctcacg ggtggagctg
     3961 gacaccaccg ccaccttgct ggtcagcgga tcggcgccgc ccagcccgtc gatctggcgc
     4021 ggatcggggc tgcccatcac gcccagcagg aaggcatcgc gcgtggcggt atcggcgggc
     4081 agatcgtggg cgaggaaata gccgcccttc gaggtgccgc cgcgcatcca catgcagggg
     4141 gcggaaagag aggtggtcat ccggagatcc tttgcggggg aggcagatgc gggagaaaca
     4201 ggcggcgcca gggggcaggc agccccttgg cgcccccggc atcagacgta cttcaggcct
     4261 tccttctcca gccgctcgcg catgctgtag atgtcgaggc cgagcacgcc ggcggccagc
     4321 ttctcgcgct tggccgcttc gttggcctcg cgggcctggg ccttttccag cacttcggcg
     4381 gcttcctcgc ggcgcaccac caccacgccg tcatcgtcgg cgacgatcac gtcgccggcg
     4441 ttcaccagct ggttggcgca gatcaccggc acgttcaccg agccgatggt gttcttcacg
     4501 gtgccctgcg cgtagatcgc cttggaccac acggggaagt gcatctcggt caggtcgcgc
     4561 acatcgcgca cgccggcatc gatgaccagg ccccggcagc cgcgcgcctg ggcgctggtg
     4621 gccagcaggt cgccgaaata gccgtcttcg caagggctgg tgggggccag cagcaggata
     4681 tcgccggcct gcagctgctc gatcgccaca tgcaccatcc agttgtcgcc cggggcggcc
     4741 gaaatggtga cggcagaacc ggcaatgcgc gcgccgcgat agatgggccg catgtagctg
     4801 gcgagcaggc ccttgcgccc ctgcgcctcg tgcacggtgg ccacgccgca ggcggccagg
     4861 ccgtcgatca cgctggcatc ggcgcgggca atgttctgga caacgatacc gcccatttct
     4921 catatctcct tcattgggca ggccctgcca gaaccaaggc aagggccggc cgcatctagc
     4981 gcgtcccgag gctcgtgccg caggttttgc ctgctggccg cttctggccg gctccatgcg
     5041 cgcgggcgca gccggccacc aatgggaagt tggcagggcg cataagattt tgctaaggct
     5101 tgccccatgg acaagaaagc cgcgccagaa cggccactca atatccggca tttcgcggcc
     5161 ctggccgcca ccgtgcgcca tggcagcctg acgcgcgccg cgcgcagcgt gaacctgacc
     5221 cagccagcgc ttacccaggc catcgccggg ctggaagcca gccttggcgt caccctgttc
     5281 gagcgggggc cgcagggcat ggccgccacc gagccggcgc tgctgatggc cccgcgcgcg
     5341 gaagcggcga tcgcccatgt cggcagcccg cgggtcaccg gcacgcaatt gcgcgccctg
     5401 ctggcgctgg cgcgggccgg ttcctatgcg gccgcggccg aggccaccgg cctttccacc
     5461 gccagcctgc accgggcggt ggccgatctg caggtggcgc tggggcagcg gctggtggac
     5521 aggcgcgggc atcatgtgct gctcaccgcg gccggccagc ggcgcgcgcg cggcttcagc
     5581 ctggccatgg ccgaactgcg cgcgggcctt gccgaagtgg ccgcctggca gggcaaggcg
     5641 gccgggcgca tcgttatcgg cgccatgccg ctgtcgcgcg cgcgctggct gccagaggcg
     5701 gtcaaccgct tcatcaccgg ccggccgggg gtggatatcg tggtggtgga aggcagccat
     5761 gccgaactgg ccggcccctt gcgcgatggc gagatcgacc tgatgctggg cgccctgcgc
     5821 ggcgagggcg agaccgacgg gctggtggaa gaggccgtgt tcgaggacca gccggtggtg
     5881 gtgatgcgcg ctggccatcc gctgctggcg cagggcgagc cctatgcccg gttgctcgat
     5941 tacccgtgga tcctgcccgg atcggaaacg ccgctgcgcc gccattggga agccatgctg
     6001 cgcgcgctgg gccatgtgcc gccgcatgtt tcggtggaat gcggatcgac catggcgctg
     6061 cggcaattgc tgctgacagg cgaagggctg acgctgctca gccccgaaca ggtggcggtg
     6121 gaactgcgcg ccggcctgct ggccacgctg gcgccgccct tgcgctttgc ccgcaccatc
     6181 ggcatcacca cgcgggccgg ctggcggccc accggcgcgc aggcgcaaat ggtggcgctg
     6241 ctgcgcttct gcggcgcgga ggtggatggc gcctgagggc ccattcgcga attgttatgg
     6301 atgtgcgcgt cattcgattg gcgcgtttgc gcggctttgc ccatggcctt gggccgattg
     6361 gcgaggaggc gtgaagtgac aggcgtaatc gctctggtcg gctatggcga ggcgggcagc
     6421 acctttgccc gcgcgggcga ctggccggcg caaacaggcg tgtgggatgt gcaggcggag
     6481 cggcgggctg tggccggcgc cgatggcctg cgcgcggctt cctcggctgc agaggcgctg
     6541 gccggggccg atctcgtgct cagcctggtg actgccgaca gcgcgctgcc ggccgcgcgc
     6601 gattatgccg cgctgctgaa gccgggcgcg atctgggccg atggcaattc cgtggcgccc
     6661 gaaaccaagc gcgccgctgc cgctgccatc gaggcggccg gcgggcgcta tgtcgatgtg
     6721 gccattctgg cgccggtgca cccggcgcgg ctcaacgtgc cgctgctgct agcaggcgct
     6781 gccgccggcg cggcgcaggc gctgctggct gatctgggtt ttgcccatat ccgcatcgtg
     6841 ggggcagaag tgggccgggc cagcgcgatc aagatgatcc gctcggtgat ggtgaagggc
     6901 accgaggcgc tgaccgccga gatgatgctg gctgcccagg ccgcaggcgt ggtggacgaa
     6961 gtgctcgcca gcctggatgc cagcgagaag cccaggccct gggccgaaaa atccgcctat
     7021 aacatcgagc ggatggtgac gcatggcctg cgccgcgccg ccgagatgga cgaatccgcc
     7081 aagaccctgg aaggccttgg cgtggagccg atgatgacgc gcggcacggt gctgcgccaa
     7141 cgtgacatgg ccggccgcac agaccggctg atcgaggaaa aggagtgatc cgcccgtgac
     7201 catggtgatc gactgccacg gccattacac cgtgctgccc aaggcgcatg acgcctggcg
     7261 cgaggagcag aaggcggcct tcaaggccgg cactgccgcg cctgcctatc ccgccatctc
     7321 cgacgacgag atccgcgaga cgatcgaggc caaccagctg cgcctcatcc gcgagcgcgg
     7381 cgccgacatg accatcttca gcccgcgcgc cagtgccatg gccccccatg tgggcgacca
     7441 gagcgtggcg gtgaagtggg cgcaggtgtg caacgacctc atcggccgcg tcgccgcgct
     7501 ctaccccgag gtgttcgcgc cggtctgcat gctgccgcag agccccgagg cggatctcac
     7561 ctcctcgatc gccgagctgg agcgctgcgt cacgcagatg ggcttcatcg gctgcaacct
//
LOCUS       DASRYE010000103        13303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_93011,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000103 DASRYE010000000
VERSION     DASRYE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93011"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1958
                     /locus_tag="VFF98_05640"
     CDS             51..1958
                     /locus_tag="VFF98_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HZU63145.1"
                     /translation="MASASTPAMAQPRSAPAAAVPAPALPAAQDKPWPAAPITLAVDA
                     TDTERGLFRTTETIALPAGTTRITLVLPRWTPGDHKPSLGLGQLADLHFTAHGEELRW
                     QRDPVEFAAFHVEVPAGTSELVASFVHTSPLQASEGQILMTPDMLNLEWWHMALYPAG
                     YFVRQIPIRPSVKLPAGWQAASALDGATRAGDTITYGLTDFETLVDSPVFAGSHARRL
                     DLGEGIALNLFADKPDQLEISASHIAAYKALASEAVSLFGARHFDHYDLLLGLSDKLG
                     AVGLEHHRSSENTMLPRALVDWAEMDWDRNVIPHELTHSWNGKFRRPADLWTPDYTTP
                     MQDSLLWVYEGQTQFWGHVLAARAGVQSKDVVLGMIASNAGQFTQWPGRGWRAVADTT
                     NDPLITGRRPLPFGSIERGEEYYTEGMLMWLEVDQTLRQGTHGARGLDDFARAFFGVR
                     DGDWGDLTYTRADVVATLNTIMPYDWAGFLTARIDQPGRPAPLAGIEMGGYRLVWKDE
                     PNPFDKGRAAQSHMLGLGNALGITLDKDGRVVGTLWGSPAFTAGIVTGAKVIAVNGTA
                     YEAEALKKAITAAKGSDKPIELTVQRGDKVQTIAIPYHDGLRWPWLERAAPGKAPTGL
                     DQLLAPRRKGA"
     gene            1958..2611
                     /locus_tag="VFF98_05645"
     CDS             1958..2611
                     /locus_tag="VFF98_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HZU63146.1"
                     /translation="MAAGRAWLLPPRFAGFMALMAIGVALALPLVERPSQALLAGFDA
                     GVLGFLASLVPLVRKRSAADMRAHASANDSGRLGMLAITGVLMLVIATGVAVELPDAR
                     RESGPAHVAALVLVLASLVLAWLFSNVVYALHYAHMYYRGAAEGGLGFPHHNDQPHAP
                     DYWDFLYFSLTIGMAFATSDVTIGRGDIRRIVTLHGAAAFFYNLIVLAFTVNVTAGG"
     gene            2756..3289
                     /gene="ppa"
                     /locus_tag="VFF98_05650"
     CDS             2756..3289
                     /gene="ppa"
                     /locus_tag="VFF98_05650"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZU63147.1"
                     /translation="MDISKISVGKNPPENLNVIIEVPTGGEPVKYEFDKESGALFVDR
                     ILHTPMRYPANYGFVPHTLSPDGDPLDALVVARSPFVPGSVVRARPIAVLNLEDEAGG
                     DEKLLCVPDDKTFPYYANVAEKEDLPDIISQQIEHFFTHYKDLESEKWVRVGTWGNAE
                     EARKIVLEAIERYQAAK"
     gene            3432..3743
                     /locus_tag="VFF98_05655"
     CDS             3432..3743
                     /locus_tag="VFF98_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63148.1"
                     /translation="MGDMQKGTTASVEPFPALAGSSVVTDELNSIWAMLKSACPAHAQ
                     ISFLFDGQLHVHIDVQKREDVIAVKTVLATLGLGLFHTITSGGTPHHPFFHRISARVN
                     R"
     gene            complement(3765..5111)
                     /locus_tag="VFF98_05660"
     CDS             complement(3765..5111)
                     /locus_tag="VFF98_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZU63149.1"
                     /translation="MLSKDEARDKAQALIERAIRAGADAADGVYIADSAESVGVRLGR
                     LEDVSRSESEHIGLRVFVGRRSASIGSSDLSDAALAELAARAIDMARAAPEDPYAGLA
                     PEERLLKAPPVDLDLIDAEEPTPAQLRALAEAAEEAARAIAGVTNSEGAGASSSSAVM
                     ALATSHGFAGAYAGTSRSVSASVVAGEGSGMQRDYGWRAARHGEDLPAPDAIGRIAGE
                     RAVARLNPGRLKSGPMPVVFDPRVGGSLVGHLVGAMSGAGIARRASFLLGREEEQLFD
                     SAIRIIDDPLRARGLRSRPFDGEGLPTAPRDLVAGGRLTGWLMESASARQLGLAPTGH
                     ASRGGNGAPGVAPSNLHLAAGTASVAALIADIEEGVLVTELIGHGVNGVTGDYSRGAS
                     GLRIVRGEIAGPVAEFTIAGNLVDMFRALSVADDLEWHRTINVPTIRIDGMMVAGD"
     gene            complement(5241..5672)
                     /locus_tag="VFF98_05665"
     CDS             complement(5241..5672)
                     /locus_tag="VFF98_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63150.1"
                     /translation="MLELGAGSMTGTIAHRAVEQEQGWLLQGQEEASPPASWPRRMSD
                     NTAYALLAYTSLQIFVTIGALRDGSSSLLPYLALVLLVVGILPACHWFEQRWSGLGAE
                     NACDPALAGRFRRDRAMLWALALGMPFAITGLIGALGLLLG"
     gene            5931..6713
                     /gene="lptB"
                     /locus_tag="VFF98_05670"
     CDS             5931..6713
                     /gene="lptB"
                     /locus_tag="VFF98_05670"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZU63151.1"
                     /translation="MTDTLQLSPSDAGAATGDDVFSGLEVVSIAKSYDGRSVLSDISL
                     RVAKGEVMGLLGPNGAGKTTCFYSIMGLVRPDSGRILLDGVDITRLPMYRRSILGLGY
                     LPQETSIFRGLSVEQNIACVLELMEPDAAIRAAELERLLEEFGLTRLRTSPAMALSGG
                     ERRRCEIARALAARPSIMLLDEPFAGIDPLSIADIRHLVHDLKERGIGVLITDHNVRE
                     TLDICDRACIIYGGQVLFAGSPEALVADENVRRLYLGEGFTL"
     gene            6767..8278
                     /gene="rpoN"
                     /locus_tag="VFF98_05675"
     CDS             6767..8278
                     /gene="rpoN"
                     /locus_tag="VFF98_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZU63152.1"
                     /translation="MAMTPRLDLRQTQSLVMTPQLQQAIKLLALSNLEVETFIGEALE
                     GNPLLEVGAGEAAPPEDALPEEARRTSLESSPVDQLIGEGRAGDDRPLDIDPAALDTD
                     RDTGDAASRPAMADGLAEGLAREGGSGEAPGIDERGAGEETLAAHLHAQIGAVARGPR
                     ALFIARFLIDHLDEAGYLATPLRELAETLDVELAEVEAALALVQLLDPTGVGARSLAE
                     CIALQAREADRYDPAMARLIDHLDLVARGEFARLRRMCGVDEEDFADMLAELRGYDPK
                     PGLRFGGGAAAAVVPDLLITARADGGWNVAINQATLPRLMVNRSYYVELKGACAGKEA
                     RGWLSEKLADANWLVKALDQRQKTILKVASAIVRAQDGFFRNGVSALKPLTLRAVAET
                     VSLHESTVSRVTSNKYLACDKGTFELKYFFTSGVASADGEGAVSAEAVKAAIRQLIDA
                     EDPKAILSDDALVDLLKAKGFDLARRTVAKYREAIGLGSSVQRRRQKALAGLR"
     gene            complement(8285..8953)
                     /locus_tag="VFF98_05680"
     CDS             complement(8285..8953)
                     /locus_tag="VFF98_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZU63153.1"
                     /translation="MPQPSYPVLALLAAKARFYPAFNEPSAIAKQPLQGRVTITREGI
                     LGDEQADRRYHGGPDKALHHYAFDHHASWAQSWGEALPLGQPGGFGENIATTGLVEEQ
                     VLLGDRFRLGTALIEVSHGRQPCFKLNHRFNRASVVAEIVKTGRCGWYYRVIEEGHCE
                     VGAGVDLRLEQVAEGLAQWPVARLFRVLVGGGHKADSAALAELAALPQLAEAWRTRAR
                     ELQG"
     gene            complement(8973..9581)
                     /locus_tag="VFF98_05685"
     CDS             complement(8973..9581)
                     /locus_tag="VFF98_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZU63154.1"
                     /translation="MLKAELIERAARFRSAGEDPSRQLADCPALVLNADFTPLSYYPL
                     SLWPWQTAIKAVFLERVDIIANYQREIHSPSWSMKLPSVIALRQYVRPSEFPAFTRFN
                     VFLRDKFSCQYCGSPHHLTFDHVVPRRLGGRTSWENIVAACSPCNLKKGGRTPAEAAM
                     PLLMWPIRPTSWQLQERGRAFPPNYLHETWRDWLYWDVELEA"
     gene            9712..10548
                     /gene="gluQRS"
                     /locus_tag="VFF98_05690"
     CDS             9712..10548
                     /gene="gluQRS"
                     /locus_tag="VFF98_05690"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZU63155.1"
                     /translation="MTITRFAPSPNGALHLGHAFAAIVAHDLARARAGHFRLRIEDID
                     GARSRPAMAAQALDDLAWLGLAWDGLVVRQSARLGAYQAAADALRGQGLLYPCSCTRA
                     EIAAAATAHGPDGPLYPGTCRGRAAAAGVPPEAIAWRLDMAAATRLAGPLAWEDEIAG
                     PQVATPGIFGDIVLMRRDAPASYHLAATLDDAADGITLVTRGRDLFAASHVHRLLQAL
                     LGLPVPRWHHHALIVEADGQKLAKRRGSPGLADLRLAGADGAALAADLRAGRLPAGLS
                     LA"
     gene            10586..10816
                     /locus_tag="VFF98_05695"
     CDS             10586..10816
                     /locus_tag="VFF98_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63156.1"
                     /translation="MSWLLGLVIAVLMALVVGALVKGVIAFLRTTREDLETHQGAGPS
                     PMQLRQNEMMFARIKYQALAIVVVAILLAATR"
     gene            10849..11418
                     /locus_tag="VFF98_05700"
     CDS             10849..11418
                     /locus_tag="VFF98_05700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZU63157.1"
                     /translation="MVKLNKIYTRTGDDGTTGLVDGSRSPKHAPRMTAIGAVDEVNSA
                     LGLACVALAGSAYAAPLARIQNDLFDLGADIATPEADFAPSPMALRIVPGQVAWLESE
                     IDGLNADLAPLTSFILPGGSEAAARVHLARAIARRAERDLCAAAAEVAINPQALAYLN
                     RLSDYLFVLARAINQGASGDVLWVPGASR"
     gene            11496..12365
                     /locus_tag="VFF98_05705"
     CDS             11496..12365
                     /locus_tag="VFF98_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU63158.1"
                     /translation="METVAVIGAGQMGAGIAQTAAQAGLAVLLSDISLELAEKAKAKI
                     GKGLDRLVAKDKLSAEAATALLGRITPVGDYAPLAGADIIIEAATEREDIKLKIFEAA
                     GKVLKADGILATNTSSISITRMSRAAPDQGRFIGMHFFNPVPVMKLVEVIPGLATSAQ
                     TTAATVALGEKMGKEVVLAQDDPGFVVNRIFVPFLNEAMFVLGNGTGSVADIDKGVKL
                     GLAHPMGPLELADFIGLDTLLEIMKVYLTETGDSKYRPAPLLQRYVAAGWLGRKTGRG
                     FYDYSGDTPVPTR"
     gene            complement(12375..12653)
                     /locus_tag="VFF98_05710"
     CDS             complement(12375..12653)
                     /locus_tag="VFF98_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63159.1"
                     /translation="MLIPRPRLNQVFKSRWMALLWAGAMLLSAYFTVEEVAGRQQAPA
                     ESSAAADSDAASAASQLQAILPADSASSAAPARSPWAPDTSTDTPAGR"
     gene            complement(12686..>13303)
                     /locus_tag="VFF98_05715"
     CDS             complement(12686..>13303)
                     /locus_tag="VFF98_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZU63160.1"
                     /translation="VGASDNLARGRSTFMVEMVETAAILSQAGPRSFVILDEVGRGTS
                     TYDGLALAWAVAEAVHGLNRCRCLFATHYHELARLAESCEALSLHHVRAREWKGDLVL
                     LHELAEGPAESSFGLAVARLAGVPAGVVARARQVLGRLEKGRAQTGGLAAGLGDLPLF
                     AATMAEPEPAADPLREQLAGIDVDALSPREALDVLYALKAAAGAG"
BASE COUNT         2131 a         4657 c         4447 g         2068 t
ORIGIN      
        1 ttcttcgcca tcgcgcccgc ctcggcctgt gcgccgccag ccttgccttc atcgcttccg
       61 ccagcacccc ggccatggct cagccccgct cggcgccggc agcggccgtg cccgcccccg
      121 ccctgcccgc cgcccaggac aagccctggc ccgccgcccc catcaccctg gcggtggatg
      181 ccaccgacac cgagcgcggc ctgttccgca ccaccgagac cattgccctg cccgccggca
      241 ccacccgcat cacgctggtg ctaccgcgct ggacaccggg cgatcacaag ccctcgctgg
      301 ggctgggcca gctggccgac ctgcatttca ccgctcatgg cgaagaactg cgctggcagc
      361 gcgatccggt ggaatttgcc gccttccatg tggaagtgcc ggccggcacc agcgaactgg
      421 tggccagctt cgtccacacc tcgccgctgc aggccagcga gggccagatc ctgatgacgc
      481 ccgatatgct gaaccttgaa tggtggcaca tggcgctcta tccagcgggc tatttcgtgc
      541 ggcagatccc catccgcccc agcgtgaagc tgccggccgg ctggcaggcg gccagcgcgc
      601 tggatggcgc cacccgcgcg ggcgacacga tcacctatgg cctgaccgat ttcgagacgc
      661 tggtcgattc gccggtgttt gccggcagcc atgcccgccg gctcgatctg ggcgagggca
      721 ttgccctcaa cctctttgcc gacaagcccg accagctgga gatctccgcc agccacatcg
      781 ccgcctacaa ggcgctggcg agcgaggcgg tgtcgctctt cggcgcccgc catttcgacc
      841 attacgacct gctgctgggc ctttccgaca agctgggcgc ggtggggctg gaacatcacc
      901 gctcttccga gaacaccatg ctgccgcgcg cgctggtgga ctgggccgag atggactggg
      961 accgcaacgt gatcccccac gagctgaccc acagctggaa cggcaagttc cgccgcccgg
     1021 ccgacctgtg gacgcccgac tataccaccc ccatgcaaga cagcctgctg tgggtctatg
     1081 aagggcagac gcagttctgg ggccatgtgc tggcggcgcg cgcgggcgta cagtcgaagg
     1141 acgtggtgct gggcatgatc gccagcaatg ccggccagtt cacccagtgg cccgggcgcg
     1201 gctggcgcgc ggtggccgat accaccaacg acccgctgat caccggccgc cgcccgctgc
     1261 ccttcggcag catcgagcgc ggcgaggaat attacaccga aggcatgctg atgtggctgg
     1321 aggtggacca gaccctgcgc cagggcaccc atggcgccag ggggctggat gatttcgccc
     1381 gcgccttctt cggcgtgcgc gatggcgact ggggcgacct cacctatacc cgcgccgatg
     1441 tggtggccac gctgaacacg atcatgccct acgactgggc cggcttcctt accgcccgca
     1501 tcgaccagcc cggccgcccg gccccgctcg ccggcatcga gatgggcggc taccggctgg
     1561 tgtggaagga cgagcccaac cccttcgaca agggccgcgc ggcgcaaagc cacatgctgg
     1621 ggctgggcaa tgcgctgggc atcaccctcg acaaggacgg acgcgtggtg ggcacgctgt
     1681 ggggcagccc ggccttcacc gccggcatcg tcaccggcgc caaggtcatc gccgtcaacg
     1741 gcaccgccta tgaggccgaa gcgctgaaga aggcgatcac cgccgccaag ggcagcgaca
     1801 agccgatcga gctgaccgtg cagcgcggcg acaaggtgca gacgatcgcc atcccctatc
     1861 acgatggcct gcgctggccc tggctggagc gcgccgcccc cggcaaggcg cccaccgggc
     1921 tggaccagct gctggccccc cgccgcaagg gcgcctgatg gccgccggcc gcgcctggtt
     1981 gctgccgccg cgctttgccg gcttcatggc gctgatggcc attggcgtgg cgctggccct
     2041 gccgctggtg gaacggccca gccaggcgct gctggccggc ttcgatgcgg gcgtgctggg
     2101 tttcctcgcc agcctggtgc cgctggtgcg caagcgctct gccgccgaca tgcgcgccca
     2161 tgccagcgcc aacgacagcg gccggctggg catgctggcg atcaccggcg tgctgatgct
     2221 ggtgatcgcc accggcgttg ccgtggaact gcccgatgcc cggcgcgaaa gcggcccggc
     2281 ccatgtggcg gcgctggtgc tggtgctggc cagcctggtg ctggcctggc tgttttccaa
     2341 tgtcgtctat gcgctgcact atgcgcacat gtattatcgc ggcgcggccg agggcggcct
     2401 gggcttcccc caccacaacg accagcccca cgcccccgat tactgggact ttctctattt
     2461 ttcgctgacc atcggcatgg cctttgccac atccgatgtc acgatcgggc ggggcgatat
     2521 ccgccgcatc gtcaccctcc acggggcggc ggcgtttttc tacaacctca tcgtgctggc
     2581 cttcacggtg aatgtgaccg ccggcggctg agatcatcag accttggtgg cagcggcgcc
     2641 atcggctgtt gcaatgcaca aggggcgcgg ctagggcctt ggccccagag gatcgctttt
     2701 ccccaaggag ccgggaccgc aaggccccct gcccttcaac ccagacgacg gtgccatgga
     2761 catcagcaag atcagcgtgg gcaagaaccc gcccgaaaac cttaacgtca tcatcgaagt
     2821 gccgacgggc ggcgagccgg tgaagtatga gttcgacaag gagtcggggg ctctgttcgt
     2881 cgatcgcatc ctgcatacgc cgatgcgcta tccggccaat tacggcttcg tgccgcacac
     2941 gctttcgccc gatggcgatc cgctcgatgc gctggtggtg gcccgctcgc ccttcgtgcc
     3001 gggctctgtg gtgcgcgcgc ggcccatcgc cgtgctgaac ctggaagacg aggccggcgg
     3061 cgatgaaaag ctgctctgcg tgcccgacga caagaccttc ccctattatg ccaatgtggc
     3121 cgagaaggaa gatctgcccg acatcatctc gcagcagatc gagcacttct tcacccacta
     3181 caaggatctg gaatccgaaa agtgggtgcg cgtgggcacc tggggcaatg ccgaggaagc
     3241 ccgcaagatc gtcctcgagg cgatcgagcg ctaccaggcg gccaagtaag cctgccatga
     3301 cagacaagcg gaagggcggg gttttccccg cccttttgtt atgcccccac ccggaaagtc
     3361 acgtagcttt tctctgcccc gccaatggcg tattatcccc taatcagagc ctgcaaggat
     3421 aggaggcctt catgggggac atgcaaaagg ggaccaccgc cagtgtcgag ccgtttccgg
     3481 cgctggccgg atcgagcgtc gtcaccgacg agctgaacag catatgggcc atgctgaaat
     3541 cggcctgccc ggcccacgcg cagatcagct tcctgttcga cgggcagctg catgtgcata
     3601 tcgacgtgca gaagcgcgag gatgtgatcg cggtgaaaac cgtgctggcc acgctggggc
     3661 tggggctgtt tcacaccatc accagcggcg gcacgccgca ccatcccttc ttccaccgga
     3721 tcagcgcgcg ggtgaaccgc tagcagcggc gatggtggag ggcctcagtc gcccgccacc
     3781 atcatcccgt cgatgcggat ggtcggcacg ttgatcgtgc ggtgccattc aagatcgtcg
     3841 gccacgctga gcgcgcggaa catgtcgacc agattgccgg cgatggtgaa ttcggccacc
     3901 ggcccggcaa tctcgccgcg cacgatgcgc agacccgagg cgccgcggct gtaatcgccc
     3961 gtcaccccgt tgacgccatg gccgatcagc tcggtcacca gcacgccttc ctcgatatcg
     4021 gcgatcagcg ccgccaccga ggccgtgcca gcggccagat gcaggttgct gggcgccacg
     4081 cccggcgcac cattgccccc gcgcgaagca tggccggtgg gcgccaggcc cagctggcgg
     4141 gccgaggcgc tttccatcag ccagccggtc aaccggccgc cggccaccag atcgcgcggg
     4201 gcggtgggca ggccctcgcc atcgaagggg cgcgagcgca ggccgcgcgc gcgcagcgga
     4261 tcatcgatga tgcggatggc gctgtcgaac agctgctcct cctcgcggcc gagcaggaag
     4321 ctggcgcgcc gggcgatccc cgccccgctc atcgcgccga cgagatggcc gaccaggctg
     4381 ccccccaccc gcggatcgaa caccaccggc atcggcccgc tcttcagccg gccggggttc
     4441 agccgggcca ccgcccgttc gccggcgatg cggccaatgg catcgggcgc gggcaggtcc
     4501 tcaccatggc gggcggcgcg ccagccataa tcgcgctgca tgccgctgcc ctcgccggcc
     4561 accacgctgg ccgacaccga gcggctggtg ccggcatagg cgccggcaaa gccatggctg
     4621 gtggccagcg ccatcaccgc gctgctggaa gaggcaccgg cgccctcgct gttggtcacg
     4681 ccggcaatgg cgcgggcggc ctcctcggcc gcttcggcaa gggcgcgcag ctgggcgggc
     4741 gtcggctcct cggcatcgat caggtcaagg tcaacgggcg gggccttcag cagccgctct
     4801 tccggggcaa ggccggcata ggggtcttcc ggggcggcgc gggccatgtc gatggcgcgg
     4861 gcggccagtt cggccagcgc cgcatccgac agatccgacg agccgatgct ggccgaacgc
     4921 cggccgacaa agacacgcag gccaatatgc tcgctttccg agcgcgagac atcttccagc
     4981 cggcccaggc gcacgcccac gctttcagcc gaatcggcga tgtagacacc atcggccgca
     5041 tcagctccgg cgcggatggc gcgctcgatc agcgcttgcg ccttgtcgcg ggcttcgtcc
     5101 ttgctgagca tgcggccttg gaactccctt catgccgcgc aggcatgaca caagcgcccg
     5161 gcggcggccc gctctagacc cgcaagggcc gaagggcaat gcgccagaca ggttccccca
     5221 gggccggaag ggcgaaggct tcagcccagc agcaggccca gcgcgccgat cagcccggtg
     5281 atcgcaaaag gcatccccag cgccagcgcc cacagcatgg cccgatcccg ccggaaccgg
     5341 cccgccagcg ccggatcgca ggcattctcg gcacccagcc cgctccagcg ctgctcgaac
     5401 cagtggcagg cgggcaggat gcccaccacc agcaacacca gcgccagata gggcagcagg
     5461 ctgctgctgc catcgcgcag ggcgccaatc gtcacgaaga tctgcaggct ggtataggcc
     5521 agcagcgcat aggcggtatt gtcgctcatc cgccggggcc agctggccgg cgggctggct
     5581 tcttcctgcc cctgcagcag ccagccctgc tcctgctcca ccgcgcgatg ggcgatcgtg
     5641 ccggtcatgc tgcccgcgcc aagctccagc attgccactc tcctgaatca tccgcctgat
     5701 gcggctttga caggaaatta atcaccgctg gccgcgccgc gcaagggcca gcgcaacggc
     5761 ccggcccgcc tgcgccaccc attggcgcgg cccgcaggcc cctggcggca aggcgctggc
     5821 gcgtttcggc cacagtggcc ggccgatcat gcaggaaacc tgccaaattg cttgccccca
     5881 tgccacccgg ccttgccgat tgcgagagcg gctcctaata agcgcgggaa atgactgaca
     5941 cgctccagct ttccccctcc gatgccggcg ctgcaaccgg cgatgacgtg ttcagcgggc
     6001 tggaggtcgt ctccatcgcc aaaagctatg atggccgctc ggtgctcagc gatatttcgc
     6061 tgcgcgtggc caagggcgag gtgatgggcc tgttgggccc caatggcgcc ggcaagacca
     6121 cctgcttcta ttccatcatg gggctggtgc gccccgattc gggccgcatc ctgctggatg
     6181 gcgtggatat cacccgcctc cccatgtatc gccgctcgat cctggggctg ggctatctgc
     6241 cgcaggaaac ctcgatcttc cgcggcctgt cggtggaaca gaacatcgcc tgcgtgctcg
     6301 aactgatgga gccggatgcc gccatccgcg ccgccgaact ggaacggctg ctggaggaat
     6361 tcggcctcac ccggctgcgc accagcccgg ccatggcgct ttcgggcggc gagcggcggc
     6421 gctgcgagat cgcccgggcc ctggccgcgc gcccctcgat catgctgctc gacgaaccct
     6481 tcgccggcat cgacccgctc tccatcgccg atatccgcca cctcgtgcat gatctgaagg
     6541 agcgcggcat cggcgtgctc atcaccgatc ataacgtgcg cgaaacgctg gatatctgcg
     6601 accgcgcctg catcatctat ggcggacagg tgctgtttgc cggcagcccc gaagcgctgg
     6661 tggccgatga gaacgtgcgc cggctctatc tgggcgaggg cttcacgctt tgatcgccgg
     6721 cagcgccccc gcgctggcag ctggccgggc cggataggcg gcggccatgg cgatgacgcc
     6781 gcgcctcgac ctgcgtcaga cccagtcgct ggtgatgacg ccgcagttgc agcaggcgat
     6841 caagctgctg gcgctgtcga acctcgaggt ggaaaccttc atcggcgagg cgctggaggg
     6901 caatccgctg ctggaagtgg gcgcgggcga ggccgccccg ccggaagacg ccctgcccga
     6961 ggaagcccgc cgcaccagcc ttgaaagctc gccggtggat cagctgatcg gcgagggccg
     7021 cgcgggcgac gaccgcccgc tcgacatcga cccggcagcg ctcgacaccg accgcgacac
     7081 cggcgatgcc gccagccggc ccgccatggc cgatggcctg gccgagggcc tggcccgcga
     7141 aggcggcagc ggcgaggccc ccggcatcga cgagcgcggc gcgggcgagg aaacgctggc
     7201 cgcccatctt cacgcccaga tcggcgcggt ggcgcgcggc ccccgcgcgc tgttcatcgc
     7261 ccgcttcctg atcgatcatc tcgacgaggc gggctatctg gccacccctt tgcgcgaact
     7321 ggccgagacg ctggacgtgg agctggccga ggtggaggcc gcgctggcgc tggtgcagct
     7381 gctcgacccg acgggcgtgg gcgcgcgcag cctggctgaa tgcatcgccc tccaggcgcg
     7441 cgaggcggat cgctatgacc cggcgatggc ccggctgatc gaccatctgg atctggtggc
     7501 gcgcggcgaa tttgcccgcc tgcgccggat gtgcggggtg gacgaggagg atttcgccga
     7561 catgctggcg gaattgcgcg gctatgatcc caagccgggc ctgcgttttg gcggcggggc
     7621 ggccgccgcc gtggtgccag atctgctcat caccgcccgc gccgatggcg gctggaacgt
     7681 ggccatcaac caggccaccc tgccccggct gatggtgaac cgcagctatt acgtggagct
     7741 gaagggggcc tgcgccggca aggaagcgcg cggctggctt tccgaaaagc tggccgatgc
     7801 caactggctg gtaaaggcgc tggaccagcg gcagaagacc atcctgaaag tggccagcgc
     7861 catcgtgcgg gcgcaggatg gcttcttccg caacggtgtc tcggcgctga agccgcttac
     7921 cttgcgcgct gtggccgaaa cggtgagcct gcatgaatcg accgtcagcc gcgtcacctc
     7981 gaacaaatat ctggcctgcg acaagggcac cttcgagctc aaatatttct tcacctcggg
     8041 cgttgcctcg gccgatggcg agggggcggt gtcggccgag gcggtgaagg ccgccatccg
     8101 ccagctgatc gatgccgaag accccaaggc gatcctgtcg gacgatgcgc tggtggatct
     8161 gctgaaggcc aaaggctttg acctcgcccg ccgcaccgtg gccaaatacc gcgaggcgat
     8221 cgggctgggc tcgtctgtgc agcggcgccg gcagaaggcg ctggccggcc tgcgctgaac
     8281 cggcctagcc ctgcaattcg cgcgcccgcg tgcgccaggc ctcggccagc tgcggcaggg
     8341 cggccagttc tgccagcgcg gcgctatcag ccttgtggcc gccgcccacc agcacgcgga
     8401 acaggcgcgc caccggccat tgcgccagcc cttcggccac ttgctccagc cgcagatcga
     8461 cgccggcgcc cacctcgcaa tggccctcct cgatcacccg gtaataccag ccgcagcgcc
     8521 cggtcttgac gatctcggcc accacgcttg cccggttgaa gcggtggttc agcttgaagc
     8581 agggctggcg gccatggctc acctcgatca gcgccgtgcc gaggcggaag cgatcgccca
     8641 gcagcacctg ctcctccacc aggccagtgg tggcgatgtt ctcgccaaag ccgccgggct
     8701 ggcccagcgg cagcgcctcg ccccagcttt gcgcccagct ggcatggtga tcgaaggcat
     8761 aatgatgcag cgccttgtct ggcccgccat gatagcgccg gtcggcctgc tcatcgccca
     8821 agatcccctc gcgggtgatc gtcacgcggc cctgcagcgg ctgcttggca atcgccgaag
     8881 gctcgttgaa ggcgggatag aagcgggcct tggcggccag cagcgcaagc acgggatagg
     8941 aaggctgggg catggccgcc atcatggggc gctcaggcct ccagttcaac atcccagtag
     9001 agccagtcgc gccaggtctc gtgcagatag ttgggcggga aggcgcggcc gcgttcctgc
     9061 aattgccagc tggtggggcg gatcggccac atcagcagcg gcatcgccgc ctcggccggg
     9121 gtgcgcccgc ccttcttcag gttgcagggg ctgcaggcgg ccacgatgtt ttcccagctg
     9181 gtgcgcccgc ccagtcgcct cggcaccaca tgatcgaaag tgagatgatg ggggctgccg
     9241 caatattggc aggagaattt gtcgcgcagg aaaacgttga agcgagtgaa ggcgggaaat
     9301 tcggaaggcc ggacatattg cctcagcgcg atcaccgagg gcagtttcat cgaccagctg
     9361 ggcgaatgga tctcgcgctg gtaattggcg atgatatcca ccctttcgag gaacaccgcc
     9421 ttgatcgctg tctgccatgg ccagaggctg aggggataat aggaaagcgg ggtgaaatcc
     9481 gcgttcaaca ccaaagcagg gcaatcggcc agttgccttg agggatcctc ccccgcgctg
     9541 cggaaacgtg ccgcgcgctc gatcagctcg gccttgagca tggcctcctg tgacacaccg
     9601 gaatgacggt ttgacgacag aacatgggcg tttttcagca cgcggggccg cccccgtcaa
     9661 gggtggacgg caagccccct tcggcataat ccacaaggca ggagcatgcc gatgaccatc
     9721 acccgttttg cccccagccc caatggcgcg ctgcatctgg gccatgcctt tgcggcgatc
     9781 gtggcgcatg atctggcccg cgcgcgcgct ggccacttcc gtctgcgcat cgaggatatc
     9841 gatggcgccc gcagccgccc ggcgatggct gcccaggcgc tcgacgatct ggcctggctg
     9901 ggccttgcgt gggatgggct ggtggtgcgc cagtcggccc ggctgggcgc ctatcaggcc
     9961 gcggccgatg ccttgcgggg ccaggggctc ctctatccct gcagctgcac ccgcgccgag
    10021 attgccgcgg ccgccactgc ccatgggccg gatgggccgc tctatcccgg cacctgccgg
    10081 ggccgggcgg cggcggcagg ggttcctccg gaagccatcg cctggcggct cgacatggcg
    10141 gcggccacgc ggctggccgg cccgctcgcc tgggaagacg agatcgccgg cccgcaagtg
    10201 gcaacgcccg gcatcttcgg cgatatcgtg ctgatgcgcc gcgatgcgcc ggccagctat
    10261 cacctcgccg ccacgctgga cgatgcggca gatggcatca cccttgtcac ccgcgggcgc
    10321 gatctgtttg ccgccagcca tgtgcaccgg ctgctgcagg cgctgctggg cctgccggtg
    10381 ccgcgctggc accaccatgc gctgatcgtg gaggccgatg gccagaagct ggccaagcgg
    10441 cgcggatcgc cggggctggc cgatctgcgc ctggccgggg ccgatggcgc ggcccttgcc
    10501 gccgatttgc gcgccggccg cctgcccgct ggcctttccc ttgcctgagg cgtagtctgc
    10561 ggcaaacaac aagggagaga aggccatgtc gtggctgctt gggctggtca tcgcggtgct
    10621 gatggcgctg gtggtgggcg cgttggtgaa gggggtgatc gccttcctgc gcaccacgcg
    10681 tgaggatctg gaaacgcatc aaggggcggg ccccagcccg atgcagctgc gccagaacga
    10741 gatgatgttt gcccgcatca aatatcaggc gctggcgatt gtggtggtgg cgattttgct
    10801 ggcagcgact aggtaaggcc ctcgcccccc cccagcgcga ggataaccgt ggtcaagctc
    10861 aacaagatct acacccgcac cggcgatgat ggcaccaccg gccttgtcga cggatcgcgc
    10921 agccccaagc acgccccgcg catgaccgct atcggcgcgg tggacgaggt gaacagcgcc
    10981 cttggcctgg cctgcgtggc gctggctggc agcgcctatg cagcgccgct ggcccgcatc
    11041 cagaacgacc tgttcgatct gggcgccgat atcgccacgc cggaggccga ttttgccccc
    11101 tcgcccatgg cgctgcgcat cgtgcccggg caggtggcct ggctggaaag cgagatcgac
    11161 gggctgaacg ccgatctcgc cccgctcacc agcttcatcc tgcccggcgg cagcgaggcg
    11221 gccgcgcgcg tgcatctggc ccgcgccatc gcccgccggg ccgagcggga tctgtgcgcg
    11281 gccgcggccg aggtcgcgat caatccgcag gcgctggcct atctcaatcg cctttcagac
    11341 tatcttttcg tactggcgcg cgccatcaac caaggcgcta gtggcgatgt gctgtgggtg
    11401 ccgggggcca gccgctgaac ggtctgcgcc cggccgctgc catgcacatc ggcaaggatc
    11461 tggccagata agcctggaag gagtttgaaa tggcgattga gacggttgca gtgattggcg
    11521 caggccagat gggcgcaggc attgcgcaaa cggcggccca ggcggggctg gcggtgctgc
    11581 tgtccgacat cagcctggag ctggccgaga aggccaaggc caagatcggc aaggggctgg
    11641 accggctcgt cgccaaggac aagctgagcg cagaggccgc cacggcgctg ctgggccgaa
    11701 tcaccccggt gggcgattat gccccgctgg ccggcgccga catcatcatc gaggccgcca
    11761 ccgagcgcga ggacatcaag ctcaagatct tcgaggcggc cggcaaggtg ctcaaggccg
    11821 atggcatcct ggccaccaac acctcgtcga tctcgatcac ccgcatgtcg cgcgcagcgc
    11881 ccgatcaggg ccgcttcatc ggcatgcatt tcttcaaccc ggtgccggtg atgaagctgg
    11941 tggaagtgat ccccggcctt gccacctcgg cgcagaccac cgctgccact gtggccctgg
    12001 gcgagaagat gggcaaggaa gtggtgctgg cgcaggatga ccccggcttt gtcgtcaacc
    12061 gcatcttcgt gcccttcctc aacgaggcga tgttcgtgct gggcaatggc accggcagcg
    12121 tcgctgatat cgacaagggc gtgaagctgg gcctggctca tccgatgggg ccgctggagc
    12181 tggccgattt catcgggctc gacacgctgc tggagatcat gaaggtctat ctgaccgaga
    12241 ccggcgacag caaataccgc cccgccccgc tgctgcagcg ctatgtggcg gccggctggc
    12301 tgggccgcaa gaccggccgc ggcttctacg attattcggg cgacacgccg gtgcccaccc
    12361 gctgaaggcc cccctcagcg cccggccgga gtatcagtcg acgtgtcggg cgcccagggg
    12421 gatctggcag gggcggcgct gctggcgctg tcggccggca ggatcgcctg cagctggctg
    12481 gcagctgatg cggcatcgct gtcggctgcc gcgctgcttt ctgccggggc ctgctgccgg
    12541 ccggccactt cctccacggt gaaataggcg ctgagcagca tggcgccggc ccacagcaac
    12601 gccatccagc ggcttttgaa cacctgattg aggcgggggc gggggatcag catggcccct
    12661 gcctagccgc cggccggtta aggaatcacc ccgcgccggc cgctgccttc agcgcataaa
    12721 gcacatccag cgcctcacgc ggggacagcg catcgacatc gatgccggca agctgctcgc
    12781 gcagcggatc ggcagccggc tctggctctg ccatggtcgc ggcaaacagc ggcagatcgc
    12841 ccagcccggc ggccagcccg ccggtttgcg cccggccctt ctccagccgg cccaatacct
    12901 gccgagcccg cgccaccacg cccgccggca cccctgccag ccgcgccacg gccaggccaa
    12961 acgagctttc cgccggcccc tccgccagct cgtgcagcag caccagatcg cccttccatt
    13021 cgcgcgcgcg cacatggtgc aggctcaagg cctcgcagct ttcggccagc cgggccagtt
    13081 cgtgataatg ggtggcaaac aggcagcggc agcggttgag gccatgcacc gcctcggcca
    13141 ccgcccaggc cagcgccaga ccatcatagg tggaagtgcc gcggcccact tcatcaagga
    13201 tgacaaagct gcgcgggccg gcctgcgaga ggatcgcggc cgtctccacc atttccacca
    13261 tgaaggtgga ccgcccccgc gccagattgt cggacgcgcc aac
//
LOCUS       DASRYE010000104         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_6167,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000104 DASRYE010000000
VERSION     DASRYE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6167"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1139
                     /locus_tag="VFF98_05720"
     CDS             27..1139
                     /locus_tag="VFF98_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZU63161.1"
                     /translation="MAATTHAIPRAQVLDAIARLTDNLVNIKDETGEFLLRLEDGRII
                     DTKGWNDWEWTHGVGLFGLWRYYEVTGDVAARRVVEEWFANRFAAGTPTKNINTVAPF
                     LTLAYLYEQNGDISFLPYFDAWAQWLMAPDGLPKTEEGGFQHIVYNDENPQEMWDDTL
                     MMSVLPLAKIGLLRGRPHYVEEAKRQFLLHVKYLADRRTGLWFHGWTFDGRHNFAQAL
                     WARGNCWVTIAIPEFIEMLDLPADDAVRLFLIETLAAQAKALAALQDESGLWHTLLDD
                     PTSYLEASATAGFAYGLLKAVRKGYLPRSYEAVAIRAIEGVLAHIAPSGELTQVSFGT
                     AMGDTLQFYKDIALTSMPYGQSLAICALGEFLRTYI"
     gene            1151..>2265
                     /locus_tag="VFF98_05725"
     CDS             1151..>2265
                     /locus_tag="VFF98_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63162.1"
                     /translation="MAPLSRPVRLVNYLAYGANDMLGAGSMAIVSGWVLLFYTRFCGL
                     SAGQAAIIFATARVIDAVLSPMIGQISDHLGRTALGRRLGRRRVFILAAIPLLPSFAL
                     MWLPGQTFWYYLVSYVAFEMVYALEIIPYETLAAEMASDYATKARLAGTRILFGQVSA
                     ILAGYAPLWLIGWLGRDNGATFFWMAAIFTVLFMAVAALLYAFSWERPHDPATLPAAP
                     LGPGAILRGLAGDLAATLRVRAFRLHLGMYLGGYISQDIFNATFTFFVLFALGGTTAA
                     ASGLLGGMYVVQLAAVALAIGMALRSSPARAYRLAAMGFGAGTVLLVGGWMAGWRVGA
                     GGLWVAIALAGLGRGMLNYIPWATYNYMADVDEIISG"
BASE COUNT          334 a          744 c          753 g          434 t
ORIGIN      
        1 ttcttctttc cctttcggag tcccccatgg ccgccaccac ccatgccatc ccccgcgcgc
       61 aggtgctcga cgccatcgcc cggctgaccg acaatctggt caacatcaag gacgagaccg
      121 gcgagttcct gctgcgcctc gaggatggcc gcattatcga caccaagggc tggaacgact
      181 gggagtggac gcatggcgtg ggcctgttcg gcctgtggcg ctattacgag gtgacgggcg
      241 atgtggccgc gcgccgcgtg gtcgaggaat ggttcgccaa ccgctttgcc gccggcacgc
      301 ccaccaagaa catcaacacc gtggccccgt tcctgacgct ggcctatctg tacgagcaga
      361 acggcgacat cagcttcctg ccctatttcg acgcctgggc gcagtggctg atggcgcccg
      421 atggcctgcc caaaaccgag gaaggcggct ttcagcacat cgtctataat gacgagaacc
      481 cgcaggagat gtgggacgac acgctgatga tgagcgtgct gccgctggcc aagatcggcc
      541 tgctgcgcgg ccgcccccat tatgtcgagg aggccaagcg ccagtttttg ctgcatgtga
      601 aatatctggc cgaccggcgc acgggcctgt ggttccacgg ctggaccttt gatggccgcc
      661 acaattttgc ccaggccctg tgggcgcgcg gcaattgctg ggtcacgatc gccattcccg
      721 aattcatcga gatgctggac ctgcccgcag acgatgccgt ccgcctgttc ctgatcgaga
      781 cgctggcggc gcaggcaaag gcgctggccg ctttgcagga cgagagcggc ctgtggcaca
      841 cgctgctcga cgaccccacc agctatctcg aggcatcggc caccgccggt tttgcctatg
      901 ggctgctcaa ggccgtgcgc aaaggctatc tgccgcgcag ctacgaagcg gtcgccattc
      961 gcgccatcga gggcgtgctg gcccatatcg cccccagcgg cgagctgacg caagtgagct
     1021 ttggcacggc gatgggcgac acgctgcaat tctacaagga catcgccctt acctcgatgc
     1081 cctatgggca aagcctggcg atctgcgcgc tgggcgaatt cctgcgcacc tacatctgaa
     1141 agcacaggcc atggcgcccc tctctcgccc cgtccggctg gtcaactatc tggcctatgg
     1201 cgcgaacgac atgctgggcg ccggctcgat ggcgatcgtg tcgggctggg tgctgctgtt
     1261 ctacacccgc ttttgcggcc tgagcgccgg gcaggcggcc atcatctttg ccaccgcgcg
     1321 ggtgatcgat gcggtgctct cgcccatgat cgggcagata tcggaccatc tggggcgcac
     1381 ggcgctgggc cggcggctgg ggcggcgccg ggtgttcata ctggcggcca ttccgctgct
     1441 gcccagcttt gcgctgatgt ggctgccggg gcagaccttc tggtactacc tcgtctccta
     1501 cgtcgccttc gagatggtct acgccctcga gatcatcccc tacgagacgc tggccgccga
     1561 aatggccagc gattacgcca ccaaggcgcg gctggcgggc acgcgcattc tgttcgggca
     1621 ggtatcggcc attctggcgg gctatgcgcc gctgtggctg atcggctggc tggggcgcga
     1681 caatggcgcc accttcttct ggatggcggc catcttcacg gtgctgttca tggctgttgc
     1741 ggccctgctc tatgccttca gctgggagcg gccgcatgat ccggccaccc tgccggccgc
     1801 ccccctgggg ccgggcgcca tactgcgcgg cctggccggc gatctggcgg cgaccttgcg
     1861 ggtgcgggcc ttccggctgc atctgggcat gtatctgggc ggctatatca gccaggacat
     1921 cttcaacgcc accttcacct tcttcgtcct ctttgccctt ggcggcacga cggcggcggc
     1981 atcggggctg ctgggcggca tgtatgtggt gcaactggcg gccgttgcgc tggccatcgg
     2041 catggcgctg cgctccagcc cggcgcgggc ctatcggctg gcggccatgg gctttggcgc
     2101 gggcacggtg ctgctggttg gcggatggat ggcgggctgg cgcgtggggg ccggcggcct
     2161 gtgggtggcc atcgcgctgg cggggttggg gcgcggcatg ctcaactaca ttccctgggc
     2221 cacctacaac tacatggccg atgtcgacga gatcatcagc gggca
//
LOCUS       DASRYE010000105        29753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_77143,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000105 DASRYE010000000
VERSION     DASRYE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 29753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29753
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77143"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFF98_05730"
     CDS             complement(<1..440)
                     /locus_tag="VFF98_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU63163.1"
                     /translation="MSGAALDPVALAARLIRRPSVTPKDEGALDVVATALEKLCFICH
                     RLVFADSGGEPIHNLYARHGAGRPNLCFAGHTDVVPVGAREAWSFDPFAAVVRDGELC
                     GRGAVDMKGAIAAFIAAAQRYIDRCQAGVAGSISLLITGDEEGRG"
     gene            complement(437..1279)
                     /gene="dapD"
                     /locus_tag="VFF98_05735"
     CDS             complement(437..1279)
                     /gene="dapD"
                     /locus_tag="VFF98_05735"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZU63164.1"
                     /translation="MTDIAALTSAIEAAWDARDTVTPASADVRADVETALALLDSGAA
                     RVAEKVDGAWHVNQWLKKAVLLSFRLNDNGLMENGMGGAPAWDKVPLKTASWGEDAFR
                     AAGFRAVPGSVVRRGSFIGKGVVLMPSFTNIGAYVGEGTMVDTWATVGSCAQIGKNVH
                     LSGGVGIGGVLEPLQAGPVIIEDNCFIGARSEVVEGVQVGEGAVLSMGVFIGASTKIV
                     DRATGEVHIGRVPPYSVVVPGSLPGKPLPDGTPGPSLYCAVIVKTVDEGTRARTSINE
                     LLRE"
     gene            1440..2675
                     /locus_tag="VFF98_05740"
     CDS             1440..2675
                     /locus_tag="VFF98_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZU63165.1"
                     /translation="MHSLQSLAGMALILLIAFALSSDKRAIRPRVVLAAFALQAGFAA
                     LVLYFPPGNAALQLVSAGVGNLLGYAHAGVMLLFGPLARPEVGGNSFAISSLPVIIFF
                     ASLISVLYYVGIMPLVVRWIGGALEKITGISRLESLCAASNIFLGQSEAPLVIRPYLA
                     GLAPAQLFCVMSVGLAGVAGTILAAYAGLGIRIDYLVAASFMSAPGGICMAKIIMPDP
                     KGAIIEPDEIVLPTSAEQGERPANIIMAASEGAQTGVKLAVAVGAMVLAFVALVALAN
                     GIVGWVGHFFGVPQLSFQLVLGYVFAPVFYLLGTPDWAEAMRAGGMFGTKVVLNEFVA
                     FIDLGQAQGLSARTVGIVTFALCGFANFSSIAIQMAVTGSLAPNQRPVIARLGMKALA
                     AGALSNLMSAALAGLFLGL"
     gene            2681..3259
                     /locus_tag="VFF98_05745"
     CDS             2681..3259
                     /locus_tag="VFF98_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZU63166.1"
                     /translation="MDWEDHWEQLGPPAPPPRARKPGVWTIALVVLGLMGLLLAALHW
                     RGWLAAGRQALAGGAPVTAAADLPRPAPADRDAAHFSLCAGPHRVNCVIDGDTIWYAG
                     EKIRLADINAPEVSDPDCDAEQLLGEKATDRLLALLNMGAFSLVPLPGRDTDVYGRKL
                     RTISRGGQSLGMVLVKEGLAEKWVGFRRDWCD"
     gene            complement(3294..4616)
                     /locus_tag="VFF98_05750"
     CDS             complement(3294..4616)
                     /locus_tag="VFF98_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZU63167.1"
                     /translation="MYPGVPASTDAPQPFHADAALAARVVVVGLGYVGLPLAVALATR
                     FETVGLDIDTRRIAELNTGHDRTGEITPERLAASTLALAAEAAICPPADYYIVTVPTP
                     IDGANRPDLRLVEAASRTVGAMLAAAVAEGRVPVVIYESTVYPGVTEETCAPILEAHS
                     GLVCGRDFFLGYSPERINPGDREHTIDKITKVVSGQTPEVLERVAQLYGAITTGGVFR
                     ASSIKAAEAAKVIENAQRDINIAFMNEIAQIFSKMDISIWDVLAAARTKWNFLPFAPG
                     LVGGHCIGVDPYYLSHRAEQLGMDPRVILSGRGINDGMAFWVAERLHEARGDVPGSVL
                     VLGFTFKENIPDLRNSKVADLVTALGRLGHAVTVHDPHGDPAEARHEYGIPLVAGALE
                     GRRYDLVILAVPHREYLALSPESLRALVAEGGLLADLKGVVAGADWTL"
     gene            4734..5876
                     /gene="wecB"
                     /locus_tag="VFF98_05755"
     CDS             4734..5876
                     /gene="wecB"
                     /locus_tag="VFF98_05755"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZU63168.1"
                     /translation="MTSAPTRPRILLIFGTRPEAIKLFPLVHALRADSRVEARVCVSA
                     QHRGMLDQVLEIAGITPDHDLDLMRAGQSLDSLTAALITGLGRVMDEEQPARVIVQGD
                     TATAMAGALAAYYRKIPVDHVEAGLRSGNIYHPWPEEVNRKIIGGIAALHFAPTETAA
                     AALARENVAAEAIHVTGNTVIDALHWVSARIAAEPQLAAGLAELEARFAGKRIIGVTS
                     HRRENFGGGLENIAEAIRQIAQRPDVAIIFPVHPNPNVRAVMDGALAGLANVAMIEPL
                     DYPHFARLLGLAEIMLTDSGGVQEEAPALGKPVLVMRETTERPEGVAAGTARLVGTDV
                     GTIVSEIFNLLDDKAAYEAMARAHNPFGDGHAARRITEILAGVTGA"
     gene            5873..7174
                     /gene="wecC"
                     /locus_tag="VFF98_05760"
     CDS             5873..7174
                     /gene="wecC"
                     /locus_tag="VFF98_05760"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HZU63169.1"
                     /translation="MKSDVKPDVCVVGLGYIGLPTAAIIARAGCRVLGVDVTQSVVDT
                     INRGEIHIEEVDLDGLVQGVVARGLLSASTAMAPADVFVIAVPTPFDKQHQPDISYVL
                     AAARSVAAVLKPGDLVILESTSPVGTTEEMRDMIAAMRPDLKVPGLTQETPDIAIAYC
                     PERVLPGRILEELTNNDRSIGGITPRCARKALAFYKRFVRGACITTDARSAEMTKLVE
                     NAYRDVNIAFANELSIVADRMGLDVWEVIRLANRHPRVNILNPGPGVGGHCIAVDPWF
                     IVAGAPEETPLIRTARGVNDGKIHHVIARAEALVEAHPQAKVACLGLAFKANIDDFRE
                     SPARLVAATLARRFHERIHIVEPYAAQLPLEFTGTGAKAIDLDEALEECDILIVLVDH
                     DLFRSVPLAERAHKLVYDTRGIWPDQPATGEGAVAALRLAS"
     gene            7274..8134
                     /locus_tag="VFF98_05765"
     CDS             7274..8134
                     /locus_tag="VFF98_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63170.1"
                     /translation="MSDLGRREPVPEGATVLLGGMPRAGTTLLCAMLNEQPGCVALAE
                     PMRPGRHGDGARALADIAAFARGTRRDLLAGRPVVSTGGGAAVDNFVPMAPAGSALRV
                     VEAPLAAWSDASPNAGSLRLFIKQPGLVAALAGALSADYPVYLVLRHPLAVLASWQTV
                     PMAVRAGHMPVAESLRPDLAARLARAGDALGRQVVLLAWLLEAALAHPRGQVLLYEEF
                     VAEPAYWLAPLAGGRWQAGAQRRPHDRLCRYAGQDLARLAHALWPLVGLAQPFWPGFA
                     GDLAEVIAAG"
     gene            8442..9461
                     /locus_tag="VFF98_05770"
     CDS             8442..9461
                     /locus_tag="VFF98_05770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU63171.1"
                     /translation="MKNDILIVDDDLSTIQVFKKMLAEVGSARFATHGAAAIDQIRME
                     APDVILLDAEMPGMTGFEVCQVLKGEAAYADIPVIFVTAHNDHELELRGLEAGAVDFI
                     SKPVNEALLRARVRTHLRLKQMSDELRKIATIDGLTQVGNRRRFDEALQREWARGIRN
                     CEPISLLMIDVDHFKPYNDHYGHPAGDACLRLIAEALGHCATRPADVVCRYGGEEFVV
                     LLPQTPRDGAAHVATRIREAVENLCIPHGRSTVAGQLTVSIGIGAYDWDSPCWSDSAE
                     AGRSTDELRNRRDALVAAADKALYCAKRGGRNQVWMKDIDEAQGAFRVNLEMANVENL
                     SARAR"
     gene            9554..10942
                     /locus_tag="VFF98_05775"
     CDS             9554..10942
                     /locus_tag="VFF98_05775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZU63172.1"
                     /translation="MDLLHPDAAGPRKRALLHGQDARAVDAAAPADRPAGAAGQPAIV
                     TQAAIVTQPAGVAQPAVAPVVSPALPVPPAAVAPDAYAVARADAPAGGEAPPPPSLSE
                     DAALIALSLPVGVVVVDAQGRIVMVNQRLCDMFGYPGADLRGKSLEVLLPERYRQGHN
                     ALRAAYHAAPVARMMGRGRDLTGLRADGMEFPIEIGLGALPTSRGQLVLATVVDITER
                     KRTELQLREANAQLEEFTSVASHDLRSPLRGVSNLIDFILDDYGDTAPVGVLRNLDRV
                     KDRIGRMEQLIEDLLAYARAGRRSAKLELIDLEDIVEEMVRLDPPPEGFRLETDLDVA
                     PFLGSATPLRTVIRNLYTNAFKHHDLKGGVIRIAARGEGDACVIEIGDDGPGIPVLVQ
                     ERVFRLFQTLAGDTAPSGTPRLGGSGLGLAVAKRLVEGHGGRIEVNSPDGVRGATFRI
                     HWPRYMRTDLDD"
     gene            10935..11357
                     /locus_tag="VFF98_05780"
     CDS             10935..11357
                     /locus_tag="VFF98_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU63173.1"
                     /translation="MTEQALSVLVVDDDDITAELVERALRRVEGAFEVISAPDGVSAL
                     AMMRGRPGGVERPYVVLLDLNMPRMNGFEFLDVLRSDPGLRDSVVFILSTSAADGDLR
                     RAYERYVAGYMVKSRVGPQFAQVSRLLRDFSETICLPQ"
     gene            11342..13732
                     /locus_tag="VFF98_05785"
     CDS             11342..13732
                     /locus_tag="VFF98_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU63174.1"
                     /translation="MPAAVIAEAAGPPAGQAAPRILVVDDDEVDREAILRLIARLDRD
                     VQVEAASSIDTAQAHLVGQDFDCIVLDYRLGGHCGLELLPWVAKLQAQVCPIVLVTDA
                     ADEELIVGAMRQGVFDYIPKSGLNLKRLGQVLEHCLAWRTLESDRRQAERRERDEKLS
                     RQREKERSLQVAVEQAENANRAKSQFVAHISHELRTPMNAVIGLTHLLERTGLTDEQT
                     ELVDKLQVAGKSLLDIIQSVLDLSKIEAGEMTLEQAPFSLFTVICEVVALIEPQARSR
                     QLAFALDLPADLPPVLLGDPVRVHRVLMNLLGNAVKFTSAGQVGLAVHALARDAAEIR
                     LRFEISDTGIGISEEAQARLFTPFVQADSSTTRRFGGTGLGLSIVKQLVELMGGQIGV
                     TSAPGAGSTFTFELTLPIADRDLPDVTHHREAEQSQRLEGVRVLLADDSEINVEIARR
                     MLELEGAQVVTAANGREAVDAVMAREGRFDVVLMDVQMPVLDGLNATRRIRSGLGLRQ
                     LPIIALTAGMLTSQRQEALACGMDDYLPKPFEVDDLIDCIRRHMRLSEADAARARSQA
                     QGGAGVAGSAEVAGPAGRPFVLGGASDWPQIAGIDPGLSERRMGGDRELLARLIEKLL
                     ELDEALAAQETTPAADEEEATAQLAAVLHKLKGSSGMLGASALQQQAGSAEAEARAGR
                     LAPARAGLGQLRAGLAQLRASSAAFLAEMAARAAARDEAQAGAGAVELGGEDITRLLR
                     QLRYNDLAALDHFERLAPALRARMGELPWRSMRDHINNLRFDEAAALLAGSAPVAS"
     gene            13828..13901
                     /locus_tag="VFF98_05790"
     tRNA            13828..13901
                     /locus_tag="VFF98_05790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13860..13862,aa:Gly,seq:ccc)
     gene            14067..15299
                     /locus_tag="VFF98_05795"
     CDS             14067..15299
                     /locus_tag="VFF98_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZU63175.1"
                     /translation="MALTDTAIRNAKPHDKEYKLADGGGLYLLITPSGGKLWRLKFRT
                     EGRERKLSIGSYPEISLSDARKRRDEAREQLANGKDPSREKQREKLRSRLQSENTFAG
                     IAKEYCEKRKRDGHKAWAPATAVRSEYLLGLLNGALGRMPIGEIEPADVLAAIRKIEA
                     KGNLESARRTQQLASAVFRYAVATARLSSDPTRDLRGAVASPKVTHYGAITQPLQVGA
                     LLRSIDGYEGMGITKIAMQLAPHVFVRPGELRHAEWSEIDLDKAIWAIPADKTKMRKP
                     HYVPLSRQAVTLLGKAYAITGPMGYVFPALHTRLRPLSENTLNSVLRRLGYTSQEMTA
                     HGFRAMASTLLNESGKWSPDAIERALAHGDSDKVRAAYHRGAHWEERVKMAQWWSDHL
                     DMLRDGADILPLHTRNAS"
     gene            complement(15584..16255)
                     /locus_tag="VFF98_05800"
     CDS             complement(15584..16255)
                     /locus_tag="VFF98_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63176.1"
                     /translation="MRTATRRRHILLAAAGLCAAGQASAQVTTCMGIGPNMVNCNTTG
                     PAPNQTHLLSRISGMIADANDRSIRKHVGELLAAGDCEGASHYALSKGRLELGEQIAQ
                     SCQPYVAPPPRPIQAAPAAPDLATMLSRTAVAIPSMQLLLDDQATLVRAETVGTQLML
                     TMRGSRPRGALSEAARSRLVGALCSHSAFIPLLHYGASVRLVYVDQGGRQNDAVMVTR
                     QICGF"
     gene            16372..16620
                     /locus_tag="VFF98_05805"
     CDS             16372..16620
                     /locus_tag="VFF98_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU63177.1"
                     /translation="MDESAILAELGRRIRAQRKRVGLTQEQLALIAGVDRSYCGSIER
                     GERNITFTVLCRLAAAMEADIAALTTGLPAPVTEMAAR"
     gene            16871..18139
                     /locus_tag="VFF98_05810"
     CDS             16871..18139
                     /locus_tag="VFF98_05810"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU63178.1"
                     /translation="MSIPGVSQAQIEAVLREHLTPSAHITTPERLFGRQKMLTQIQRA
                     FNSEGRHVFIFGDRGVGKTSLAMTAAYLSQSSEHTPIYVLCSGQSTFSDIMVSIGQNA
                     FPPTEFMESKAKSGKLALTAVGFGGTYEAPTPSTISIEAPRDINEACNIIKFINSKRP
                     KQNVIIIDEMERIESQDEKIKIAEFLNSISSVQSNSKFILCGIGQSVEDLLGAHRSAT
                     RKLETIGLERINHSELWKILSTAADALDVSLNTDFLMRASILSDGFPHYVHLIGESLF
                     WAMFDDENDCISATTDHFRSAVKGALSRTEVEHRTAYQKATEKTKNTKEYEISLWALA
                     DKTETRRQLDNIYRDSYLRIMREFYPTEKPMDKTRFNQRLLSLKTQNHGEIIRGFGSG
                     WFSFRETILRGYVRLIAENQDVYLAKDASQ"
     gene            18210..18557
                     /locus_tag="VFF98_05815"
     CDS             18210..18557
                     /locus_tag="VFF98_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63179.1"
                     /translation="MAASEFRWALDAIDRAHWDRIYIDHLPHGACGHCAELLASYLQQ
                     RFGVTADYVCKDFYDNDGVRETSHAWLEWYGLIIDISSDQSNCPAVIVTHQSDLHARG
                     EDELRHAFRVDPA"
     gene            complement(19108..20940)
                     /locus_tag="VFF98_05820"
     CDS             complement(19108..20940)
                     /locus_tag="VFF98_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63180.1"
                     /translation="MKAFTDLNLGFTNAENYRRRANKELLAKYFVRDEYLEKLLDPNV
                     YFLVGEKGTGKTAYSTYLRNNRYKEKHAFAYDVRQTEYQKFLELKRLGHLPLSQYSEV
                     WRTLLLILSATSVLEKSSTPDFLKKFTKLGALKRAIDDFYDNAFAPEIVKILNFVESS
                     ERSTSLMAKYGGLHGGTDSKNKVEIKDSSSTFQTNLLTIRQSFEDAIEALRLDTDHII
                     FIDGIDVRPAEIGYDDYFECVRGLVDAVWSLNNDFLADIKDSRGRIRVVLLVRPDIFL
                     RTGLHNVNTKIRDNSVFLNWSTTYKDYRTSSLFRVADRLLSAQQDEVLESGKAWDHYF
                     PFHAENVRDFQFSSSSVLTSFLAFLRFSYYRPRDINAMLGTMKDILLRRGATPYVSAD
                     DFSDPTFRDAHADYLLGEIRDQLLFYYSQEEFDLFLLFFQYLKGKKKFTYDEFTNAVT
                     EFVAECVSSGKVVPKFFESVDVFLQFLYEQNVICYIETDDERSDSERFIRWCFRERTP
                     SNLSPKVRSHVEYEIFYGLTKALNVGRSIKTKKPNSKREFGTVVGISPDEKFGFIRGG
                     IAQIDHYFQFSQLKAGYSPRVGDRVSYSPAVKYGKLRAHAIQRA"
     gene            complement(21439..22485)
                     /locus_tag="VFF98_05825"
     CDS             complement(21439..22485)
                     /locus_tag="VFF98_05825"
                     /inference="COORDINATES: protein motif:HMM:NF042598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6765 family protein"
                     /protein_id="HZU63181.1"
                     /translation="MDIEFHYYMTYLIATHAGFAPADAATIATASQEVDDNHIAIVVN
                     QGTPEAYASTVSQTMDITKPHHDLRIYPIFHFIPGEPNAPSARRQDGQTSVWMTTPNS
                     PLAQAMLQTALSSGSLHRIGASSHAYVDTWAHQNFIGKDDPLNEMIKPDSLIEEFKQK
                     VFKIGHALANHDPDIPDLIWTDGRLATPTVVNADRFMDAAQHLYAVYCQHTKKPVVSA
                     DVARLVGDLRADIGTPSPTSASPETRISRYKARALQPAYGGHPIPDYREGAWQDAAFA
                     EQRANLGNEIDTWVELHTGLASDVLTVAPMTYTWKNPATRQDTDWFKFQEAVKAHLDE
                     CWGLLVQRIPQAQA"
     gene            complement(22680..23180)
                     /locus_tag="VFF98_05830"
     CDS             complement(22680..23180)
                     /locus_tag="VFF98_05830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU63182.1"
                     /translation="MHIREAKGADLAGILAIYNHAVLHTTAVWSEAPATLEDRHIWLA
                     AKQQRRFPVLVAVAGEEVLGFASYGDFRPWPGYAHSVEHSVYVAPACHRRGVGKALVQ
                     ALIDHAHNAGYHTMIAGIDASNAPSIALHGALGFTETGRLPEVGRKFGRWLDLAFMQK
                     MLNGTR"
     gene            complement(23521..25023)
                     /locus_tag="VFF98_05835"
     CDS             complement(23521..25023)
                     /locus_tag="VFF98_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HZU63183.1"
                     /translation="MSDRIKELFDNAEIIEPQAPLPLRRDTAPPQPYPIKALGKVLAG
                     AALGIMDKVQCPDAIAAQSVLAAAALASQAHANVVHPATKEQRPLSLFMITVAASGDR
                     KSSADKPALDPVRQREAELHDKHKQDKQSYDDARALYDREREAALKMKGPREERKKAL
                     ADLGPPPCPPLMPVLVVTEPTLEGLHKLMAAGEPSMGLFSDEGGGFIGGHAMTEDARL
                     RTATGLSDLWDGKPVKRVRGGEGVMILHGRRLAAHLMAQPGIVEGLLADPLFIQQGLT
                     SRLLVAAPASIAGTRMQRQPKLTTEPALKKYREKMLGLLRHPQPRAGPSDATLLPRSI
                     HLTEPAVEKWKAFADECERELATGGRLEPIKAFGNKLPEHALRIAAVLKLFDDAKAAE
                     ISVDTLGQAVILARYYAEEALRLFEQAAISVELQRAERLLKWLHQTWGRPQVGLSHIY
                     QKGPNSIRTAALARQAMAILEDHRWVVPLADGAKFDGKHHRDAWEVRPQP"
     gene            complement(25016..25996)
                     /locus_tag="VFF98_05840"
     CDS             complement(25016..25996)
                     /locus_tag="VFF98_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZU63184.1"
                     /translation="MSRRPAGNASGLKATVLALLPGLIRQWLPNGELRGDVYFALNPR
                     RQDRHLGSFQINIRTGRWRDHAIDVGGRDAVSLYAYLSTGGDYRAAFTALTSDPLVQA
                     AMAAGAAAPPAKPAKAAKPAADKLALVRRLYANATGLTGMPAATYLQNRGLHRTDAWE
                     TLRASVQHYPGAGACPALLASIDAPDGSLVGLHRTFLTSVGDKLGVANPRLTLGQVRG
                     GAIRLGKASDELIICEGLEDGLTLFQLLDRAVWVAGGAPFLPLMVIPEAVRILTIAAD
                     NDAAGERAARRAADVFGVGGREVRIMRPAPGFKDFNDQLRNIKSKDFDDE"
     gene            complement(25993..26157)
                     /locus_tag="VFF98_05845"
     CDS             complement(25993..26157)
                     /locus_tag="VFF98_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU63185.1"
                     /translation="MHFTFSIAEAARALSLGRTKIYELIDEGRLDTIKIGRRRLIKVA
                     SIRALLEAGK"
     gene            complement(26247..26948)
                     /locus_tag="VFF98_05850"
     CDS             complement(26247..26948)
                     /locus_tag="VFF98_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63186.1"
                     /translation="MVHHIFPPEGKVVIAELSISWVSNRLGHWRWPFPYRRRGNVEYD
                     ERSLNALKECEAILVEDLSSARLRALAHVEGVNIFYEISAEYWKEGASELSLKGRMYD
                     LSPGQSFSERLEGAAVLVTARDAITWLERQGIRDTDPYFPHCLRAPPTPGVPLRKSQY
                     ERNAFIDQAVFRLHRDGAPRGRGAQAKFEEAMRAWYEDNIGKPPKKRTIRNYVREAFR
                     RFHLENEMRLGNQSE"
     gene            complement(27326..28894)
                     /locus_tag="VFF98_05855"
     CDS             complement(27326..28894)
                     /locus_tag="VFF98_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU63187.1"
                     /translation="MSAPTAGAAVSLRAWVAVLGGVVGCFMAGMNVHVTNASLPDVRG
                     ALGASFEEGSWITTAYLVAEIIIIPMTGWLVSVFSMRRVLMVGTGGFVLFSLLCSCAP
                     DIATMIAARALQGAFGGVLIPLSLQIIVSELPPEKHPFGMALFAVSNNVAQAAGPSLG
                     GWLTQACSWRWIFYLQVPPGLLLLAAIGWAMPRAPLRISRLREGDWSGIATMALGLSA
                     LQIMLEEGERQDWFASGFIIAAAGVAAAALIAFTLIELRRPAPLINLRLLGQARFGLA
                     SLMQFVFGAIVFGVVFLVPNYFAETQGYNAAQIGLTMIPYGLVQLVMSFATPRLMRWT
                     SVRTIIIAGFLLMAAGCLMNIHLDGDAAANVIVPSLVVRGIGQSFIVVALGVMAMQGM
                     ARAETGSAAGLFATLRNVGGAIGIALASQLVVERTKFHAARMGEAVTPWSEPLRERMA
                     LAAGVLAGHPPGRHAALDGAAGAPLRQKVLALIAGNLHEQALLLAYSDAFLIAGLCML
                     ACAAASLLLRQERS"
     gene            complement(28891..>29753)
                     /locus_tag="VFF98_05860"
     CDS             complement(28891..>29753)
                     /locus_tag="VFF98_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU63188.1"
                     /translation="GAILARIDPRDYRLKVAQAQGALAVAEAGLAAATARIANLDARA
                     QQQRSTIAQNAAAVTARQAEAQLATQQAERQAHLARQQVTSEEKLQSAQADRRKAAAG
                     WTQARAALAAARAQVQVLATERAAAIAAAAGARGALQQARAGLAAARLDLERTAIRAP
                     VSGIVGQRALRLGQYADIGMPLLALVPMAPYVVANYKETQIDRIRPGQPVTVAVDALG
                     GAVLTGRVDSFAPASGAQFALLPPDNATGNFTKIVQRMPLRIRLDAGQARLGALRPGM
                     SVETSVDTRP"
BASE COUNT         5567 a         9020 c         9611 g         5555 t
ORIGIN      
        1 ccgcggcctt cctcgtcgcc ggtgatcaac aggctgatcg agccggcgac cccggcttgg
       61 caccggtcga tgtaacgctg cgccgccgcg ataaaggcgg caatcgcgcc tttcatgtcg
      121 accgcacccc ggccgcacag ctcgccgtcg cgcacaaccg ccgcaaaagg atcgaacgac
      181 caggcctcac gcgctcctac cggcaccacg tcggtgtgcc cggcaaagca gaggttgggc
      241 cggccggcac catggcgggc atagaggttg tggataggct cgccgccgct gtcggcgaag
      301 accagcctgt ggcagataaa gcacagtttc tccaaagccg tggcgacgac atcgagcgcc
      361 ccctcgtctt tcggggtgac gctcggccgg cggatcagcc gggcggcgag cgccacggga
      421 tcgagtgccg ctccgctcat tcgcgcaaca gctcgttgat cgaggtccgg gcacgcgtgc
      481 cctcgtccac cgtcttgacg atgacggcgc aatagaggct ggggccgggc gtgccatcgg
      541 gcagcggctt gccgggcagg ctgccgggca ccaccaccga atagggcggc acgcggccga
      601 tatgcacttc gcccgtggcg cgatcgacga tcttggtgga ggcgccgatg aacacgccca
      661 tcgaaagcac cgcgccttca cccacctgca cgccttccac cacttcggag cgggcgccga
      721 tgaagcagtt gtcctcgatg atgacggggc cggcctgcag cggctccagc acgccgccaa
      781 tgccaacgcc gcccgacagg tgcacattct tgccgatctg cgcgcagctg ccaacggtgg
      841 cccaggtgtc caccatggtg ccctcgccca cataggcgcc gatgttggtg aaggagggca
      901 tcagcaccac gcccttgccg atgaagctgc cgcggcgcac cacgctgccg ggcactgcgc
      961 gaaacccggc ggcgcggaat gcatcctcgc cccagctggc ggtcttcagc ggcaccttgt
     1021 cccatgccgg ggcgccgccc atgccgtttt ccatgaggcc attgtcgttc aggcggaagg
     1081 agagcagcac cgccttcttc agccactggt tgacgtgcca ggcgccatcc accttctcgg
     1141 ccacgcgggc cgcgcccgaa tcgagcagcg ccagcgcggt ttccacatcg gcgcgcacat
     1201 cggcgctggc aggggtcacc gtgtcgcgcg cgtcccaggc ggcttcgatg gcggaggtga
     1261 gggcggcgat gtcggtcatg cggggtcctt ggacaaaaag ggggcgaaat gattggcccc
     1321 tcgcctagtg cctctgccgc ccaagaacaa gcgctggcgt gatgcgcccc ctcgacgccg
     1381 ccgccgcgcc ccgatacgag aagggcggcg caaacgccac gagggggccc gaacacacaa
     1441 tgcacagcct gcagagcctg gccggaatgg cgctgatcct gttgatcgcc tttgcccttt
     1501 ccagcgacaa gcgggcgatc cggcccaggg tggtgctggc cgcctttgcg ctgcaggccg
     1561 gctttgccgc gctggtgctc tatttcccgc cgggcaatgc ggcgctgcag ctggtgtcgg
     1621 cgggggtggg caatctgctg ggctatgccc atgccggggt gatgctgctg tttggcccgc
     1681 tggcccggcc cgaagtgggc ggcaacagct ttgccatttc ctcgctgccg gtgatcatct
     1741 tcttcgcctc gctgatctcg gtgctttatt acgtggggat catgccgctg gtggtgcgct
     1801 ggatcggcgg cgcgctggag aagatcaccg gcatcagccg gctggaaagc ctgtgcgcgg
     1861 ccagcaacat cttccttggc caatcggaag cgccgctggt gatccggccc tatctggcgg
     1921 gcctggcgcc ggcgcagctg ttctgcgtga tgagcgtggg gctggcgggc gtggcgggca
     1981 ctattctggc cgcctatgcc gggcttggca tccgcatcga ttatctggtg gcggccagct
     2041 tcatgtcggc gccggggggc atctgcatgg ccaagatcat catgccagac cccaagggcg
     2101 cgatcatcga gcctgacgag atcgtgctgc caaccagcgc cgagcagggc gagcggccgg
     2161 ccaacatcat catggccgct tccgaaggcg cgcagacggg ggtgaagctg gcggtggcgg
     2221 tgggcgccat ggtgctggcc tttgtggcgc tggtggcgct ggccaatggc attgtcggct
     2281 gggtggggca tttcttcggt gtgccgcagc tctccttcca gctggtgctg ggctatgtgt
     2341 tcgcgccggt gttctatctg ctgggcacgc ctgactgggc cgaggcaatg cgcgcgggcg
     2401 gcatgttcgg caccaaggtg gtgctgaacg aatttgtcgc cttcatcgat ctggggcagg
     2461 cgcagggcct gtcggcgcgc acggtgggga ttgtcacctt tgcgctgtgc ggctttgcca
     2521 atttcagctc gattgccatc cagatggccg tgaccggcag ccttgcgccc aaccagcgcc
     2581 cggtgatcgc ccggctgggg atgaaggcac tggcggccgg cgcgctctcc aatctgatga
     2641 gcgcggcgct ggcggggctg tttctggggc tttgagggcg gtggactggg aagaccattg
     2701 ggagcagctg ggcccgccgg cgcccccgcc ccgggcccgc aagcccggtg tgtggacgat
     2761 cgcgctggtg gtgctggggc tgatggggct gctgctggcc gcgctgcact ggcgcggctg
     2821 gctggcggcg gggcgccagg cgctggcagg cggcgcgccg gtgacggccg cggccgatct
     2881 gccccgcccc gccccggcag accgggatgc ggcgcatttt tccctttgcg cagggccgca
     2941 ccgggtgaac tgcgtgatcg atggcgacac catctggtat gccggcgaga agatccgcct
     3001 ggccgatatc aacgcgcccg aagtttctga ccccgattgc gatgccgagc agctgctggg
     3061 cgaaaaggcg accgaccggc tgctggcgct gctcaacatg ggtgccttca gcctggtgcc
     3121 gctgccgggg cgcgataccg atgtctatgg ccgcaagctg aggacgatca gccgcggcgg
     3181 gcaaagcctg gggatggtgc tggtgaagga ggggctggcc gagaaatggg tgggcttccg
     3241 gcgcgactgg tgcgactgag gggaagggcg ccttctcccc tcgtcgcgcg cgctcacagc
     3301 gtccagtcgg cgccggccac cacgcctttc agatccgcca gcaggccgcc ttctgccacc
     3361 agcgcgcgca gggactcggg cgagagggcg agatattcgc ggtgcggcac ggccaggatg
     3421 acaagatcgt agcgccgccc ttccagcgcg ccggccacca gcggaatgcc atattcgtgc
     3481 cgcgcctcgg ccggatcgcc atggggatca tgcacggtga ccgcatggcc cagccggccc
     3541 agcgcggtga caagatcggc caccttggaa ttgcgcagat cggggatgtt ctccttgaag
     3601 gtgaagccca gcaccagcac gctgccgggc acatcgccgc gcgcctcgtg caggcgttcg
     3661 gccacccaga aggccatgcc atcgttgatg ccgcggcccg agaggatcac ccgcggatcc
     3721 atccccagct gttcggcgcg atgcgagaga tagtagggat ccaccccgat gcagtggccg
     3781 ccgacaaggc ctggcgcaaa gggcaggaag ttccatttgg tgcgcgcggc cgccagcacg
     3841 tcccagatgg agatatccat cttcgagaag atctgggcga tctcgttcat gaaggcgatg
     3901 ttgatgtcgc gctgggcgtt ctcgatcacc ttggcggcct cggcggcctt gatgctgctg
     3961 gcgcggaaga cgccgcctgt ggtgatcgcg ccatacagct gcgccacgcg ttccagcacc
     4021 tcgggcgttt gtccggagac gaccttggtg atcttgtcga tcgtgtgctc gcggtcgccc
     4081 gggttgatgc gttcggggct gtagccgagg aagaaatcgc gcccgcacac caggccggaa
     4141 tgggcctcga ggatgggcgc gcaggtttct tccgtcacgc cggggtaaac ggtcgattcg
     4201 tagatcacca ccggcacccg cccttcggcc accgccgctg ccagcatggc gcccaccgtg
     4261 cggctggccg cttccaccag ccgcaggtcg ggccggttgg cgccgtcgat gggggtgggc
     4321 acggtcacga tgtagtaatc ggccggcggg cagatcgccg cttcggccgc cagcgccagc
     4381 gtgctggccg cgaggcgctc aggcgtgatc tcgccggtgc ggtcgtggcc ggtgttcaac
     4441 tcggcgatgc ggcgggtgtc gatatcgagg cccaccgtct cgaagcgggt ggccagcgcc
     4501 acggccagcg gcaggcccac atagccaagc cccaccacca ccacgcgggc ggccagcgcc
     4561 gcatcggcat ggaagggctg gggcgcatcg gtgctggcag gaacgccggg atacatcacg
     4621 gtcaaggcgg gtgccctttt tggctgcggg cacggatttc gcgccccttt gccgccattt
     4681 agcgtcaagc ctcttatttc atggttaacc cgtaggaggt tatgcgaagg gccatgacaa
     4741 gcgcacccac ccgcccccgc atcctgttga ttttcggcac ccggcccgag gcgatcaagc
     4801 tgtttccgct ggtgcacgcg ttgcgtgccg attcgcgcgt cgaggcgcgg gtctgcgttt
     4861 cggcgcagca tcgcggcatg ctcgatcagg tgctggagat cgccggcatc acgcccgacc
     4921 atgaccttga cctgatgcgc gcaggccaga gcctcgattc gctgaccgcc gcgctgatca
     4981 ccgggctggg ccgggtgatg gatgaggaac agcccgcccg cgtgatcgtg cagggcgata
     5041 ccgccaccgc catggccggc gcgctggccg cctattaccg caagatcccg gtggaccatg
     5101 tggaagccgg cttgcgttcg ggcaatatct atcacccctg gcctgaggaa gtgaaccgca
     5161 agatcatcgg cggcatcgcc gccctgcatt tcgcccccac cgaaaccgcc gccgctgccc
     5221 ttgcgcgcga gaatgtggcg gcggaggcga tccatgtcac cggcaacacg gtgatcgatg
     5281 cgctgcactg ggtttccgcc cgcattgccg cagagccgca gctggccgcc ggccttgccg
     5341 aactggaagc gcgctttgcc ggcaagcgga tcatcggcgt taccagccac cggcgcgaga
     5401 atttcggcgg cgggctggag aatatcgccg aggccatccg ccagatcgcc cagcggcccg
     5461 atgtggcgat catcttcccg gtgcacccca accccaatgt gcgcgcggtg atggatgggg
     5521 cgctggccgg ccttgccaat gtggcgatga tagagccgct ggattatccg cattttgccc
     5581 gcctgctggg gctggccgag atcatgctca ccgattcggg cggcgtgcag gaagaggcgc
     5641 cggcgctggg caagccggtg ctggtgatgc gcgagacgac cgagcggccc gagggcgtgg
     5701 ctgccggcac ggcgcggctg gtgggcaccg atgtgggcac catcgtttcc gaaatcttca
     5761 atcttctcga cgataaagcc gcttacgagg cgatggcgcg ggcccacaat ccgtttggcg
     5821 atgggcatgc cgcccggcgc atcaccgaaa tcctcgcagg agtgaccggg gcatgaaatc
     5881 tgacgtgaag cccgatgtgt gcgtggtggg cctgggctat atcggcctgc ccacagcggc
     5941 gatcattgcc cgcgcgggct gccgcgtgct gggcgtggat gtgacgcaaa gcgtggtgga
     6001 caccatcaac cgcggcgaga tccatatcga ggaagtggac cttgatggcc tggtgcaggg
     6061 cgtggtggcg cgcgggctgc tttcggccag caccgccatg gcgccggccg atgtgttcgt
     6121 catcgccgtg cccacgccgt tcgacaagca gcaccagccg gatatctcct atgtgctggc
     6181 cgcggcccgc agcgtggcgg cggtgctgaa gccgggcgat ctcgtcatcc ttgaatcgac
     6241 ctcgccggtg ggcaccaccg aggaaatgcg cgacatgatc gcggccatgc gccccgatct
     6301 caaggtgccg ggcctgacgc aggaaacgcc cgacatcgcc atcgcctatt gccccgagcg
     6361 ggtgctgccc ggccgcatcc tcgaagagct gaccaacaac gaccgctcca tcggcggcat
     6421 cacgccgcgc tgcgcgcgca aggcgctggc cttctacaag cgcttcgtgc gcggggcctg
     6481 catcaccacc gatgcgcgca gtgccgagat gaccaagctg gtcgagaacg cctaccgcga
     6541 cgtcaacatc gcctttgcca atgaactgtc gatcgtggca gaccggatgg ggctggatgt
     6601 gtgggaagtg attcgcctgg ccaaccgcca tccgcgcgtc aacatcctga acccggggcc
     6661 cggcgttggc ggccattgca ttgcggtgga tccgtggttc atcgtggccg gcgcgcccga
     6721 ggagacgccg ctgatccgca ccgcgcgcgg ggtgaacgat ggcaagatcc accatgtgat
     6781 cgcccgcgca gaagcgctgg tagaggccca tccgcaagcg aaggtggcct gcctgggcct
     6841 ggccttcaag gccaatatcg acgatttccg cgagagcccg gcgcggctgg tggcggccac
     6901 cctggcccgc cgcttccacg agcggatcca tatcgtcgag ccttatgccg cgcagctgcc
     6961 gctggaattc acaggcaccg gcgccaaggc gatcgatctc gacgaggcgc tggaagaatg
     7021 cgacatcctc atcgtgctgg tcgatcatga tctgttccgc tcggtgccgc tggccgaacg
     7081 cgcgcacaag ctggtttacg acacgcgtgg catctggccc gatcagccgg ccacaggcga
     7141 gggcgcggtg gccgcgctga ggctggccag ctgagcgcct atccttcgga aaccgaggct
     7201 ttggcgcctg cgctgagggg gcaggcgcgc taacccctgc caggcggctg tgcgggccgc
     7261 cgggggaaag cgggtgagcg atctggggcg gagggagccg gtgccggaag gcgccacggt
     7321 gctgctgggc ggcatgccgc gggcgggcac cacgcttttg tgcgccatgc tgaacgaaca
     7381 gccgggctgc gtggccctgg cagagccgat gcgcccgggc cgccatggcg atggcgcccg
     7441 cgcgctggcc gatattgccg cctttgcccg cggcacccgg cgcgatctgc tggccgggcg
     7501 gccggtggtc tccacaggtg gcggggcggc ggtggataat ttcgtgccga tggcgccggc
     7561 cggatcggcg ctgcgcgtgg tggaagcgcc gctggccgct tggtcggatg cttcaccaaa
     7621 cgccgggtcg ctgcgcctgt tcatcaagca gcccgggctg gtggcggcgc tggcaggggc
     7681 gctgtcggcc gattatcccg tctatcttgt cttgcgccat ccgctggcgg tgctggcctc
     7741 gtggcaaacc gtgccgatgg ccgtgcgcgc ggggcatatg ccggtggccg aaagcctgcg
     7801 ccccgatctt gcggcgcggc tggcgcgggc gggcgatgcg ctgggccggc aggtggtgtt
     7861 gctggcctgg ctgctggaag cggcgctggc ccatccgcgc gggcaggtgc tgctctatga
     7921 ggaatttgtg gcagagccgg cttattggct ggcgccgctg gcgggggggc gctggcaggc
     7981 gggggcgcag cgccgcccgc atgatcggct gtgccgctat gccgggcagg atctggcgcg
     8041 gctggcccat gcgctgtggc cgctggtggg gctggcgcag ccgttctggc cgggctttgc
     8101 gggcgatctg gccgaggtga tcgccgccgg gtgagccgca ggtgttaaga atctggccga
     8161 tatctttatg aataagagat tgtacgtatt ctataaaatc gattgtttaa cggacaatac
     8221 gtatggttga cctatcaaat atgcaccaat ttataaacta taagaccttt gtcgaaaggc
     8281 tcttccgcat attgtggggg cgatgaattt ctgtttgccg ggccgtttgc ctgggcgcga
     8341 cggaaaatcg ttggaacata cataatcagg tcgcgcacac aataacgagc cggtttcggt
     8401 gtcattcctg caggtcaggg tcagatcaag agtgcgaagc catgaagaat gatatcctta
     8461 ttgttgacga tgacctgagc acgatccagg tcttcaagaa gatgctggcc gaggtgggca
     8521 gcgcccgttt cgccacgcat ggcgccgccg cgatcgacca gatccgcatg gaggcgcccg
     8581 atgtcatcct gctggatgcc gagatgccgg ggatgactgg ctttgaggtg tgccaggtgc
     8641 tgaagggcga ggcggcctat gccgacattc cggtgatctt cgtcaccgcc cacaacgacc
     8701 acgagctgga actgcggggc ctggaagcgg gcgcggtgga tttcatcagc aagccggtga
     8761 acgaggcgtt gctgcgggcc cgcgtgcgca cccatctgcg cctgaagcag atgagcgacg
     8821 agctgcgcaa gatcgccacg atcgacgggt tgacgcaagt gggcaaccgc cgccggtttg
     8881 acgaggcgct gcagcgcgaa tgggcgcgcg gcatccgcaa ttgcgagccg atcagcttgc
     8941 tgatgatcga tgtcgatcac ttcaagccct ataacgacca ttatggccat ccggcgggcg
     9001 atgcctgcct caggctgatt gccgaggcgc tgggccattg cgccacgcgg ccggccgatg
     9061 tggtgtgccg ttatggcggc gaggaattcg tggtgctgct gccgcagacc ccgcgcgatg
     9121 gcgcggccca tgtggccacg cgcatccgcg aggcggtgga gaacctgtgc atcccgcatg
     9181 gccgctctac cgtggccggg cagctgacgg tgagcatcgg cattggcgcc tacgactggg
     9241 acagcccctg ctggagcgat tccgccgagg ccggccgcag caccgatgaa ctgcgcaacc
     9301 ggcgcgatgc gctggtggcc gcggccgaca aggcgctgta ttgcgccaag cgcggcggcc
     9361 gcaaccaggt gtggatgaag gatatcgacg aggcccaggg cgccttccgg gtgaatctgg
     9421 aaatggccaa tgtcgagaat ctgtcggcgc gggcgcgcta ggcccggcca ttccgccaga
     9481 atggaggtga ccgggctgga cgtgacgtct ggcctggtgc cagttcaggg aagcagaaag
     9541 aaggaagaca ataatggacc ttctgcatcc ggatgccgcc ggcccccgca agcgcgcgtt
     9601 gctgcatggg caggatgccc gcgcggtgga tgcggccgcg ccggcggatc ggccggctgg
     9661 tgcggcgggc cagcctgcca tcgtgaccca ggctgccatc gtgacccagc ctgccggcgt
     9721 ggcccagcct gccgtggcgc ccgtggtttc gcctgctctg cccgtgccgc cggcggcggt
     9781 ggccccggat gcctatgcgg tggcccgcgc cgatgccccg gcggggggag aggcaccgcc
     9841 gccgcccagc ctcagcgagg atgccgcgct gatcgccctg tcgttgccgg tgggggtggt
     9901 ggtggtggat gcgcaggggc gcatcgtcat ggtcaaccag cgcctgtgcg acatgtttgg
     9961 ctatccgggt gcggatctgc gcggcaaatc gctggaagtg ctgctgccgg agcgttatcg
    10021 ccagggccac aatgccttgc gcgcggccta tcacgcagcg ccggtggcgc ggatgatggg
    10081 ccggggccgt gacctcaccg ggctgcgcgc cgatggcatg gaatttccca tcgagatcgg
    10141 gctgggcgcg ctgcccacct cgcgcgggca gctggtgctg gcaacggtgg tggacattac
    10201 cgagcgcaag cgcaccgagc tgcagctgcg cgaggccaat gcccagctgg aggaattcac
    10261 ctcggtggcc tcgcatgatc tgcgctcgcc gctgcgtggg gtgagcaacc tgatcgattt
    10321 cattctggat gattatggcg atacagcgcc cgttggcgtg ctgcgcaacc tggaccgggt
    10381 gaaggaccgc atcggccgga tggaacagct gatcgaggat ctgctggcct atgcgcgggc
    10441 cgggcggcgt tcggccaagc tggagctgat cgatctggag gatatcgtcg aggaaatggt
    10501 gcggctggat ccgccgcccg aaggcttccg gctggaaacc gatctcgatg tggcgccctt
    10561 cctcggctcg gccacgccgc tgcgcacggt gatccgcaac ctctacacca atgccttcaa
    10621 gcatcacgat ctgaagggcg gggtgatccg cattgctgcc cggggcgagg gggatgcctg
    10681 tgtgatcgag attggcgacg acgggccggg cattcccgtg ctggtgcagg aacgggtgtt
    10741 ccgcctgttc cagacgctgg cgggcgatac cgcgccttcg ggcacgccgc ggctgggcgg
    10801 cagcgggctg gggctggccg tggccaagcg gctggtggaa ggccatggcg ggcggatcga
    10861 ggtgaactcg cccgatggcg tgcgcggggc cactttccgc atccactggc cgcgctacat
    10921 gaggacggat ctggatgact gagcaggcgc tgtccgtgct ggtggtggac gatgacgaca
    10981 tcaccgcaga actggtggag cgcgcgctgc gccgggtgga gggcgccttc gaggtgatct
    11041 ccgcgcccga cggggtgagc gcgctggcga tgatgcgcgg ccggccaggc ggggtggagc
    11101 ggccctatgt ggtgctgctg gatctcaaca tgcccaggat gaacggcttt gaatttctcg
    11161 atgtgctgcg gtccgatccg ggcttgcgcg attcggtggt gttcatcctg tccacctcgg
    11221 cggccgatgg cgatctgcgc cgcgcctatg aacgttatgt ggccggttat atggtgaaat
    11281 cgcgcgtggg gccgcaattt gcgcaagtgt cgcggctgct gcgggatttc tctgaaacca
    11341 tatgcctgcc gcagtaatcg ccgaggccgc cggcccgccc gccgggcagg cggccccgcg
    11401 cattctcgtg gtggatgatg acgaggtgga ccgcgaggcg atcctgcggc tgatcgcccg
    11461 gctggaccgc gatgtgcagg tggaggcggc cagcagcatc gataccgccc aggcgcatct
    11521 ggtggggcag gatttcgatt gcatcgtgct ggattaccgg ctgggcggcc attgcgggct
    11581 ggaactgctg ccctgggtgg ccaagctgca ggcgcaggtg tgccccatcg tgctggtgac
    11641 agatgccgcc gacgaggaac tgatcgtggg cgccatgcgc cagggcgtgt tcgattatat
    11701 ccccaaatcc ggcctcaacc tgaagcggct gggccaggtg ctggaacatt gcctggcctg
    11761 gcgcacgctg gaaagcgacc gccgccaggc cgagcggcgc gagcgcgacg agaagctgag
    11821 ccggcagcgc gagaaggaac gctcgctgca ggtggcggtg gagcaggcgg agaacgccaa
    11881 tcgcgccaag agccagttcg tggcccatat cagccatgaa ttgcgcacgc cgatgaatgc
    11941 ggtgatcggc ctgacccacc tgctggagcg caccggcctg accgacgagc agaccgagct
    12001 ggtggacaag ctgcaggtgg ccggcaaaag cctgctcgac atcatccaga gcgtgctcga
    12061 cctctcgaag atcgaggcgg gcgagatgac gctggagcag gcgcccttct cgctgttcac
    12121 ggtgatctgc gaggtggtgg cgctgatcga accgcaggcg cgcagccgcc agctggcctt
    12181 cgcgctggat ctgccggccg atctgccgcc ggtgctgctg ggcgatccgg tgcgggtgca
    12241 cagggtgctg atgaatttgc tgggcaatgc ggtgaagttc accagtgccg gccaggtggg
    12301 cctggcggtg catgcgctgg cgcgcgatgc ggcggaaatc cgcctgcggt tcgagatcag
    12361 cgataccggc atcggcattt ccgaggaagc ccaggcccgg ctgttcaccc cctttgtgca
    12421 ggccgacagt tccaccaccc gccgctttgg cggcaccggg ctgggcctgt cgatcgtcaa
    12481 gcagctggtg gagctgatgg gcgggcagat cggcgtgacc agcgcgccgg gcgcgggcag
    12541 caccttcact ttcgagctga ccctgccgat tgccgaccgc gatctgcccg atgtcaccca
    12601 ccaccgcgag gcagagcaga gccagcggct ggaaggcgtg cgcgtgctgc tggccgacga
    12661 cagcgagatc aatgtggaaa tcgcccgccg catgctggaa ctggaagggg cgcaggtggt
    12721 gacggccgcc aatggccgcg aggcggtgga tgcggtgatg gcgcgcgaag ggcgcttcga
    12781 tgtggtgctg atggatgtgc agatgccggt gctcgacggg ctgaacgcca cgcgccgcat
    12841 ccgcagcggg ctgggcttga ggcagctgcc gatcatcgcg ctcaccgccg gcatgttgac
    12901 cagccagcgg caggaagcat tggcctgcgg catggatgat tatctgccca agccgttcga
    12961 ggtggacgat ctgatcgatt gcatccgccg ccatatgcgc ctcagcgagg ccgatgccgc
    13021 gcgcgcgcgc agccaggcgc aagggggcgc tggcgtggcc ggcagcgccg aggtggcggg
    13081 gccggccggg cggcctttcg tgctgggcgg ggcgagcgac tggccgcaga tcgccggcat
    13141 cgatccgggc ctcagcgaaa ggcggatggg gggcgacagg gaattgctgg cgcggctgat
    13201 cgagaaattg ctggagctgg acgaggcgct ggccgcgcag gaaaccaccc ccgccgccga
    13261 tgaggaggag gccaccgccc agctggcggc ggtgttgcac aagctgaaag gcagctctgg
    13321 catgctgggg gccagcgcgt tgcagcagca ggccggcagc gccgaggccg aggcgcgggc
    13381 cggccggctg gcgccggcgc gggcggggct ggggcaattg cgcgcggggc tggcgcagct
    13441 gcgcgcatca agcgccgcct ttctggccga gatggccgcc cgcgccgctg cgcgcgacga
    13501 ggcgcaggcc ggcgccggcg ctgtggagct ggggggcgag gatatcaccc ggctgctgcg
    13561 ccagctgcgc tacaacgatc tggccgcgct cgatcatttc gagcggctgg ccccggcgct
    13621 gcgcgcgcgg atgggcgaat tgccgtggcg ttccatgcgc gatcacatca acaacctgcg
    13681 cttcgacgag gccgccgccc tgctggccgg cagcgccccg gtggcctcct aggcgcggcg
    13741 caagggagag ggcgccgcaa acccggttga cgcccgcgcg ccaacgtggc actggctgcg
    13801 cctgcacgcg aggcccagcg cccccgggcg ggtgtagctc aatggtagag cagcagcttc
    13861 ccaagctgaa tacgagggtt cgattccctt cacccgctcc acttcttggt tcgccggcgt
    13921 ccgtcggcat ccttgcaaac ctcagaaata cgccatattt taggcctaga tggctgacgg
    13981 catccgcttg cgttcgctgg cagctgcccc ggattggggg cctttatggg gccttttgtc
    14041 cgggggcttt ttaaaaggcc cccaacatgg cactgaccga caccgcgatc cgcaacgcca
    14101 agccccacga caaggaatac aaactggccg atggcggcgg cctttatctg ctgattacac
    14161 cgtcgggcgg caaactctgg cgtctgaagt tcaggacgga agggcgagag cggaagctgt
    14221 ccattggcag ctatcccgag atcagcctga gcgatgcacg taagcgccgc gacgaagcgc
    14281 gagagcaatt ggccaacggc aaggatccgt cccgagagaa gcagcgagag aagctgcgat
    14341 caaggctgca atcggagaac acctttgcgg gtatcgccaa ggaatattgt gagaagcgaa
    14401 agcgtgatgg ccataaggcg tgggcaccag cgactgccgt tcgcagcgaa tacctcctgg
    14461 ggctgctgaa tggcgcgttg gggcggatgc ccatcggcga gatagagccg gcagatgtgc
    14521 ttgcggcgat ccgcaaaatt gaggccaagg gtaatttgga aagcgcacgg cgcacgcaac
    14581 agctcgccag cgcggttttt cgctatgccg tggcgaccgc gcggctatcg tcggacccca
    14641 ctcgcgattt gcgcggcgct gtggcctccc ccaaggtcac ccattatggc gcgattaccc
    14701 aacccctcca agtcggggct ctgctcaggt ctatcgacgg atatgagggc atgggcatca
    14761 ccaagatcgc gatgcagttg gccccgcacg tgtttgtgcg ccctggcgaa ctgcgccacg
    14821 ccgagtggag cgaaattgat ctggacaagg ccatttgggc gatcccggca gacaagacga
    14881 aaatgcgcaa gcctcattac gtgcctctat cgcgtcaggc ggtcacgttg cttggaaagg
    14941 catatgccat caccgggccc atggggtacg ttttccctgc gctccacacc cgcctgcgcc
    15001 cattgagcga gaacacgctt aattccgtgc tgcggcggct gggctatacc agccaggaga
    15061 tgaccgcgca tggatttcgc gccatggcca gtacattgct gaacgaatcg ggcaaatggt
    15121 cgccggacgc catcgagcga gcattggccc atggcgatag cgacaaggtg cgcgcagctt
    15181 atcaccgggg cgcccactgg gaggaacggg tgaaaatggc acagtggtgg tcagatcatt
    15241 tagacatgct gcgagatggg gctgatattc tgccactgca cacacgaaac gcctcgtagg
    15301 tgcgcgaggc gtgccccgcg gcaccgggcc cacccgtttc acggccattt agtaagccta
    15361 tggaggtggt tttaccgggc gtcacggcag tcctcgattt taagtctttt tcaatattaa
    15421 ataacacctt gtggactctt ccaacgcgca aaaagctgac caaaagcatg caatgccatg
    15481 gtcgatggcc agcgcattgc ggggcaaacg gcagggcctg ctaaaaccgc taaagctgct
    15541 aaccgcaacg ggcgtttaaa actgggcggc ggcggccggg ctgctaaaag ccacaaatct
    15601 ggcgcgtcac catcaccgca tcgttctggc gcccaccctg atcgacgtaa accaggcgga
    15661 ccgaggcgcc ataatgcagc agcggtatga aagccgaatg cgagcagagc gcccccacca
    15721 gcctggagcg ggccgcttcc gacagggcac cgcgtgggcg cgagcctctc attgtcagca
    15781 tcaactgcgt gccgaccgtt tccgccctca ccagcgtggc ctgatcgtcc agcagtagct
    15841 gcatggaggg gatggccaca gcggtgcggc tgagcatggt ggccagatcc ggtgcagcag
    15901 gcgcagcctg gatcggccga ggcggcggcg ccacataagg ctggcaggat tgtgcaatct
    15961 gttcgcccaa ttccagccta cccttgctga gggcatagtg gctggctccc tcgcaatcgc
    16021 cagccgccag caattcgccc acatgcttgc ggatcgaacg atcgttcgca tcggcgatca
    16081 tgcctgaaat gcggctcaac aggtgtgttt gattaggcgc tgggccggtg gtgttacagt
    16141 tcaccatatt ggggccgata cccatgcaag tggtgacctg agcactggcc tgtccggcag
    16201 cgcataggcc ggcggcggcc aagagaatat ggcggcggcg cgttgcagtt ctcatggcga
    16261 acatccttcc cctaattagc tgcgctgcgt catggcatac ccctcaaagg ttaagaatga
    16321 ggtatattag tccgtggact gatagtcctc atttcaggcc taacctgccc gatggacgag
    16381 agcgcgattc tcgctgaatt gggcaggcgc ataagagcgc agcgtaagcg cgtgggcctg
    16441 acgcaagagc aactggcgct gatcgcgggc gtagatcggt cctactgcgg ttcaattgag
    16501 cgcggggaac ggaacattac cttcaccgtg ctgtgccggc tggcagccgc aatggaagct
    16561 gacatagcag cgctaacgac agggctgcca gcgcccgtca ccgaaatggc agcaagatag
    16621 acggggcgac tttgggggac actccggaag ggcagctccc agcagcttaa atgtgactag
    16681 cggacattcg agatggcgat ccgggggcgg ctttcggtga ggtctggatc gcttgaacgt
    16741 tggttgctgc aagtaggtga attaagtttg aaaactgaat gccgcctgac gcaagagttt
    16801 tccccattct gcgagcggca atctcttgcg tcaggcggca ttggacgtac attcggaaca
    16861 ggagattcag atgtccattc ctggtgtatc acaagcacag atcgaagctg ttctaaggga
    16921 gcaccttact ccctcggcac atataacaac gccagaacgc ctttttgggc gacaaaaaat
    16981 gctaactcag atccaaagag cattcaattc cgaaggacgc cacgtcttca tttttggaga
    17041 tcggggagtc ggcaagacct cgcttgcgat gactgctgca tatcttagtc agagttcgga
    17101 acatactcca atttatgtgc tctgcagcgg ccagtcgacg ttttctgaca taatggtgtc
    17161 tattggtcag aacgcatttc cgccaactga gtttatggag tccaaggcca agagtgggaa
    17221 actggcttta acagcagtcg gatttggcgg aacatacgaa gcgccgacac catcgacgat
    17281 atcaattgaa gccccaagag atataaacga agcttgcaat ataattaaat ttataaattc
    17341 aaaaagaccc aagcaaaatg tgattataat tgacgaaatg gaaagaatag aaagccaaga
    17401 tgagaagatt aaaattgcag aatttttgaa tagtatttct tctgttcaaa gcaattcaaa
    17461 atttattttg tgcggcattg gacaaagtgt tgaggatctg ttgggggcac ataggtcggc
    17521 aactagaaaa ttagaaacca ttggccttga acgaataaac cattcggaat tatggaaaat
    17581 tctgtctact gctgcagatg ctcttgatgt ttcactgaac accgacttct tgatgcgtgc
    17641 tagtattctg agtgacggct ttcctcatta tgttcacctc atcggagaga gtctcttttg
    17701 ggcaatgttt gacgacgaaa acgattgcat ttccgcgaca actgatcatt ttcgttcagc
    17761 tgtaaagggt gctctgagca ggactgaggt tgaacataga acagcctatc agaaagctac
    17821 cgaaaaaact aaaaatacta aagaatatga gatttccctt tgggcccttg ctgataaaac
    17881 agagactcgt cgtcagcttg ataatatata tcgtgattcc tatttaagaa tcatgcgcga
    17941 attttatcca acggaaaaac ctatggataa gacgcgcttt aaccaacggc ttctcagctt
    18001 aaaaactcaa aaccacgggg aaatcatcag gggctttggt tcaggatggt tctcatttcg
    18061 agaaaccatt cttcgaggtt atgttaggct gatagccgaa aatcaagatg tctatctcgc
    18121 aaaagatgca tctcaatgag cgtcgttgcg ggtgatccag acccacctgc ggacctgatc
    18181 gaggggggca taccgatgcc gacatcttcg tggcggcaag cgaattccgg tgggcgcttg
    18241 atgcgataga tcgggctcat tgggaccgga tctatatcga ccacctcccc catggcgcct
    18301 gcggccactg tgccgagctg cttgcatcgt atcttcaaca gcgtttcggc gtcaccgctg
    18361 actatgtttg caaggatttc tacgacaatg acggagtgcg tgaaaccagc cacgcatggc
    18421 tcgagtggta tgggctaatt atcgatattt cgtccgacca gtccaactgc ccggcggtca
    18481 ttgtaacgca ccagtcagat ctgcacgcac ggggtgagga cgagttgcgc catgccttcc
    18541 gcgtcgatcc ggcgtagtgg gggcagcagc gcgtcaggac tgagctacag tagccggacc
    18601 gaagcgtcgc cattacatcc cgcaaatgat gctacggctt tttgtagacg agcttagcca
    18661 gctttggttc tggcgaggaa ccttcgagtc cggcgagggg agcgttcagc tctggcgtgc
    18721 tagatgaacg aaggcctgct ttcaacgacc agaaaagggg ctttggaccg cagctttgtc
    18781 ggcagtcacg acgacacgtc gggcggggct ggaaccggca gcaggcgacc agctttcgcc
    18841 gttccggtga gatctctgaa atgtccgctt atgccggacc cagtcgttcg cgtgccagag
    18901 ggccgcaggt cggccctgcg cctaactcag ccggtcggct gatattagtg tttgcctagt
    18961 agcggacatt ctttcagctc gatatgaacg ccgtacgcgt ggccaacatc agccatccca
    19021 cccatcacta tagttgcggc aggcaacggt ttcgccgttg ccgccacgag tatcagtttg
    19081 aagacggacg tagaatttga tgccaaatca tgcgcgctgt atcgcgtggg cccgaagctt
    19141 tccgtacttc acggcgggag aatacgatac tcggtcgcca acgcgaggtg aatagcctgc
    19201 cttcagttga gaaaattgaa aataatgatc gatttgagca atgccgcctc tgatgaatcc
    19261 aaatttttca tcaggtgaaa ttccaactac cgttccaaac tccctttttg agttaggctt
    19321 tttcgtttta attgatcttc cgacattgag cgcttttgtt agaccataga atatctcata
    19381 ctctacatgg gacctaactt tcggtgaaag attggacggg gtccgctctc gaaagcacca
    19441 ccggataaac ctttctgaat cactccgttc atcgtcggtt tcgatataac aaattacatt
    19501 ttgctcatac aagaactgta aaaatacatc tacagattca aaaaatttag gaacgacttt
    19561 acctgaagaa acgcattcgg ctacaaattc tgttacagca tttgtaaatt cgtcataggt
    19621 aaactttttt ttgcctttca aatactgaaa gaagagcagg aataggtcaa attcttcttg
    19681 agagtaataa aatagtagtt gatccctaat ttctcctagg agataatccg catgagcatc
    19741 tctaaacgtc ggatcgctaa agtcatcagc gctaacatag ggcgtcgccc cacgacgcaa
    19801 caatatatcc ttcattgttc caagcatcgc gttaatatcg cgcggcctat agtaactaaa
    19861 ccttaaaaat gccaaaaacg aagttaagac actactagaa gagaactgaa agtctctgac
    19921 attttcggca tgaaatggga aataatgatc ccaagcttta ccagattcca gaacttcatc
    19981 ctgctgcgca gatagcaatc tatcagcaac acgaaacaga gaagatgtcc tatagtcctt
    20041 ataagtagta gaccagttca aaaacaccga attatcacgg atttttgtat tgacgttgtg
    20101 caatccagtt ctgaggaata tgtcgggccg gactagaaga acaactcgaa tacgcccccg
    20161 cgaatcttta atgtcagcga gaaagtcgtt gttcagcgac caaaccgcat cgacaagtcc
    20221 tcgtacgcat tcgaagtagt catcatagcc aatctcagct ggccgaacat caataccatc
    20281 aatgaatata atatgatcag tatctaggcg aagcgcttca atcgcatcct caaatgactg
    20341 gcgaatagtt aaaaggtttg tctgaaaagt agagctagag tcctttatct ctactttatt
    20401 tttactgtca gttcctccat gcaatccgcc gtatttagcc atcagtgatg tcgatcgctc
    20461 agaggactca acaaaattga gaatttttac gatctcagga gcgaatgcat tatcgtagaa
    20521 atcatcgatc gctcgcttca gcgcgccaag cttcgtgaac ttttttagaa aatcgggggt
    20581 actcgatttc tccaagacgc tagtggcaga taatatcaat agaagagtgc gccatacttc
    20641 agaatattga cttaaaggaa gatggcccaa cctctttaat tcgagaaatt tttgatattc
    20701 agtttgccta acatcataag cgaaagcatg cttttccttg tatctgttat tccgcagata
    20761 agtcgagtat gccgtttttc ccgttccttt ttctccgacg agaaagtaca catttggatc
    20821 gagcaacttt tcaaggtact cgtcacggac aaaatatttt gcaagcaact ctttgtttgc
    20881 acggcggcga tagttttcag cgttggtgaa ccctagattg agatcagtga atgccttcat
    20941 tgtagccaag ccttccttat gccctaatct ttccaacgtc agtcgtccga cggaatggcg
    21001 gactgcgtac tgcacaaaac cgtttcgggt aaagaggtca gtccctttac cccggttatc
    21061 tcgcgatgcg ttgctcggtc aaacaacaat gaggaatccc gccaagcatt cgcatgatgg
    21121 atgggacact gaaacacgcc ccggcaatgg cagctatcgc gacgttttgt ctccaagcaa
    21181 gacagctcct atcggctaat cccgctcatc cagtggtgtt gcacaaccct atagcctacg
    21241 acggctcatg gtcgtaaaat cgcctttcgg cgaggtatct gcgaccgaca aatttgtccc
    21301 acggccagtc gctccgtcgt tttggaagct ccctgaacga acgccagcgt ttgcaggatg
    21361 gggctaaatc ttcgaacgac cgctctgaag gcggttacgc tgactgagcg ggccgatagc
    21421 ctatgcctat gcctatgcct atgcctgcgc ctgcggaatg cgctgcacca gtaggcccca
    21481 gcattcatcc aagtgcgcct tcaccgcttc ctgaaacttg aaccagtcgg tgtcctgccg
    21541 cgttgcggga ttcttccaag tgtaggtcat cggcgcgacg gtcaagacgt cggaggcaag
    21601 gccggtatgc aattcaaccc aagtgtcgat ctcgttgcca aggttggcgc gctgctcggc
    21661 aaaggcggca tcctgccaag cgccctcacg ataatcgggg atggggtggc cgccataggc
    21721 gggttgcagc gcccgcgctt tgtatcggct gatgcgcgtc tcagggctgg ctgacgttgg
    21781 ggacggcgtg ccgatatcgg cacgcaaatc cccgaccaac ctggccacat cagcggagac
    21841 caccggcttt ttcgtatgct ggcaataaac cgcatacagg tgttgcgcgg catccatgaa
    21901 gcgatcggca ttgacgaccg tgggcgtggc aagccgccca tcggtccaga tcaggtcggg
    21961 aatgtcgggg tcgtgattgg cgagcgcgtg gccgatctta aagaccttct gcttgaattc
    22021 ttcaatcagc gaatccggct tgatcatttc gttgagagga tcatccttgc cgatgaagtt
    22081 ctgatgcgcc caggtgtcga cataggcgtg cgaactggcg ccgatgcggt gcagcgagcc
    22141 cgatgacagc gccgtttgca gcatggcctg cgccagcggg ctgttcggcg tggtcatcca
    22201 gacactggtc tggccatcct gacggcgggc gctgggtgcg ttgggctcgc cggggatgaa
    22261 gtggaagatg gggtagatgc gcaggtcgtg atgcggcttc gtgatgtcca tcgtctgcga
    22321 gacggtgctg gcataggcct cgggcgtgcc ctggttgacc acaatcgcga tgtggttgtc
    22381 gtccacttcc tgtgaggctg tggcaatggt ggccgcatcg gcaggtgcaa aaccggcgtg
    22441 cgtggcaatc agataggtca tgtaataatg gaattcgata tccatggacc accccacaag
    22501 aacgcccggc aagcctcaca cgggccaaga gtgcgttcgt gccatgcccc tgtccatagg
    22561 tcgcgcacaa tttccggagg atacagcatc gctcccgaag tagcagtata cctcatgaat
    22621 caacggtggg tttcggcgcc cagaccagag gctgggaacg gcaactctga aaggcgtttt
    22681 cacctggtgc cattcagcat tttctgcatg aaggcgagat cgagccagcg gccgaatttg
    22741 cgccccactt cgggcaggcg cccggtttcg gtaaagccca aagcgccatg cagcgcgatt
    22801 gagggcgcat tgctggcatc aatgccggcg atcatcgtgt gatagcctgc gttgtgggca
    22861 tgatcgatca gcgcttgcac caaggccttg ccgaccccgc gccgatggca ggcgggagcg
    22921 acatagaccg aatgctcgac cgaatgggca tagcctggcc aagggcgaaa atcgccataa
    22981 gaggcaaagc ctagcacctc ttctcccgcc accgccacca gcaccggaaa gcggcgctgc
    23041 tgcttggcgg ccagccagat atgcctgtct tccagcgttg ccggcgcctc gctccacacc
    23101 gccgtcgtgt gcagaacagc gtgattgtag atcgccagaa tgccggccag atctgcgccc
    23161 ttggcttcgc ggatatgcat ggccctgctt ttcacaaacg cttgataaac acaacagagc
    23221 gacaggcgcc gagcggcagg aggatgggca ggccgacctt catcgcggca ggcgtatcga
    23281 caggcatggc agctgccaca ggcgctgccc tgaggggcgc ctagaaggcc tcctaaggtt
    23341 tggctgcaca cagccccgcc cctgctcatg tggcctcctg gcgccattta gagcatgttg
    23401 atgtcgacag gcccttaaag gggggcatca gtaccggtgc cccagaaggg ataaccgcgc
    23461 gcaaaagagc cggctgacac ctcatgatgt caaccggctc tttcgtggtc tacgtagcgg
    23521 ttacggttgc ggccgcacct cccaagcatc gcggtgatgc ttaccgtcaa acttcgcccc
    23581 atccgctagc ggcacgaccc aacggtggtc ttcaagaatc gccatggctt ggcgcgccag
    23641 ggctgccgtg cggatgctgt tcggcccctt ttggtaaata tgggataagc ccacttgcgg
    23701 cctcccccac gtctgatgca gccatttcag cagcctttcc gcccgctgaa gctcaacaga
    23761 gatagccgcc tgttcgaaga gccgcagcgc ctcttcggca taataacgcg ccaggattac
    23821 ggcctggcca agggtgtcga cgctgatctc ggcggccttg gcatcatcaa acaatttcag
    23881 caccgccgcg atccgcaagg catgctcggg cagcttgttg ccaaatgcct tgatcggctc
    23941 cagccggccg cccgtggcca gttctcgctc acattcatcg gcaaacgcct tccatttctc
    24001 gacggccggc tcagtcaaat gtatggaccg gggcaaaagt gtcgcatcac tcgggccggc
    24061 gcgcggctgc gggtgacgca gaaggcccag catcttttcc cgatacttct tcagcgcagg
    24121 ttcggttgtt aactttggct gccgctgcat ccgcgtgccg gcgatgctgg ccggggccgc
    24181 caccaacaag cgcgatgtca gcccttgctg gatgaaaaga ggatcggcca gcagtccctc
    24241 gacgatgcca ggctgcgcca tcaggtgtgc ggcaaggcgc cgcccgtgca ggatcatcac
    24301 accttcaccg ccacgcacac gtttaactgg ctttccatcc cacagatcgg aaaggccggt
    24361 ggcggtacgc aggcgcgcat cttcggtcat cgcgtgcccg ccgatgaagc cgccaccttc
    24421 atcgctgaac aggcccatgg aaggttctcc tgcggccatc agcttgtgca gcccctccag
    24481 cgtcggctcg gtcaccacca gcacaggcat cagcggcggg cagggaggtg gaccaagatc
    24541 ggccagggcc ttcttccgtt cctcccgggg ccccttcatc ttaagtgctg cctccctctc
    24601 gcggtcgtaa agggcgcggg catcgtcata gctttgcttg tcctgcttat gcttgtcatg
    24661 caattcggct tcccgctggc gcaccggatc gagcgcgggc ttgtcggccg aactcttgcg
    24721 atcgcccgat gccgccaccg tgatcatgaa aagcgataac gggcgctgct ctttggttgc
    24781 agggtgaacc acattcgcat gagcctgaga cgccagggca gcggcggcga gcaccgattg
    24841 ggcggcgatg gcatcggggc actgcacttt gtccataatg cctaatgctg cgccagccag
    24901 caccttaccg agagccttga tggggtaagg ctgtggtgga gctgtatcgc gccggagagg
    24961 gagaggggcc tgcggctcga tgatctcggc gttgtcgaac agttctttga tgcgatcact
    25021 catcgtcgaa atccttggat ttgatgttgc gaagctgatc gttgaaatct ttgaagcccg
    25081 gcgcgggccg catgatgcgc acctccctcc cgcccacgcc gaacacgtcg gcggcacgtc
    25141 gagcggccct ttcgccagct gcatcgttgt cggcggcgat ggtgaggatg cggactgcct
    25201 cggggatcac catcagcggc aggaagggcg cgccgccggc cacccaaacg gccctgtcga
    25261 gcaactggaa cagggtaagg ccgtcctcca gcccctcgca gatgatcagc tcgtccgacg
    25321 ccttgcctaa ccggatggca ccgccccgca cttgcccgag cgtcaagcgc gggttggcca
    25381 cgcccaactt gtcgccaacc gaggtaagaa aggtacggtg caggccgacc agggagccgt
    25441 ctggggcgtc gatggacgcc aacagcgctg ggcaagcgcc tgctccgggg taatgctgta
    25501 cagatgctcg aagcgtctcc caggcgtctg tacggtgcaa ccccctgttt tgcaggtagg
    25561 ttgcggccgg catgccggtt aggcccgtgg cattggcata taagcgtcgc accagggcca
    25621 gcttgtctgc cgccggttta gcggccttag cgggtttagc agggggtgcc gctgcgccgg
    25681 ccgccatggc agcttgcacc agcggatcgc tcgtcaatgc cgtgaaggct gcccggtagt
    25741 caccgccggt ggacaggtag gcatagagtg acacagcgtc gcggccgcct acgtcaatgg
    25801 cgtgatcccg ccatcgaccg gtgcggatgt tgatttggaa cgacccgagg tggcgatcct
    25861 ggcggcgagg gttgagagcg aagtagacat cgcccctcag ttccccgttg gggagccact
    25921 gccggatcag gccgggaaga agcgccagaa cggtagcctt caacccgctt gcgttgcccg
    25981 ccggacgacg gctcatttac cggcctccag cagcgcgcgg atggacgcga ccttaatcag
    26041 ccggcggcgg ccaatcttga ttgtgtccaa ccgaccttca tcgatcagtt cataaatctt
    26101 ggtgcggcca aggctgagcg cgcgcgccgc ctcggcgatg gaaaaagtga agtgcatttg
    26161 tgcctccaac cgccgtgcgc tgattcgtca cggtggttag gggggtggca cgacaggggg
    26221 cattgattgg gcagtttggg caaagtttat tccgactggt tgcccaatct catctcgttt
    26281 tccaggtgaa atcggcgaaa agcttcccgc acgtagttcc gaatggtccg cttttttggg
    26341 ggcttgccga tgttgtcctc ataccaagcg cgcattgcct cctcaaattt ggcttgggcg
    26401 ccgcgccctc gcggggcgcc gtcccgatga aggcggaaaa ccgcctgatc gatgaaggca
    26461 tttcgttcat attgactttt gcgaagcggc acgcccggcg taggaggcgc tctcaagcaa
    26521 tgtggaaagt atgggtcggt atcgcggata ccttgccgct caagccaagt gatcgcatcg
    26581 cgtgcagtga cgaggacagc cgcaccttcc aatctctccg aaaacgattg tccgggagat
    26641 aaatcgtaca tcctcccctt tagagaaagt tccgatgccc cctctttcca atattctgca
    26701 gatatctcat aaaatatatt tacaccctct acgtgagcaa gcgcacgcaa tcttgctgat
    26761 gacaaatctt ctacgagaat cgcctcgcat tctttcaggg catttagaga gcgttcgtca
    26821 tactcgacat tccctctgcg ccgatatggg aatggccatc gccaatggcc caggcgattg
    26881 ctcacccagg agattgaaag ctcggcgata acgaccttgc cctcaggtgg gaaaatatgg
    26941 tgaaccatcg tagcatcccc aatgctctct agtgctcaac ccgtatggta cctcaattcg
    27001 cggtaacgat ccgcgccaca acctaccccc tgtggggacg ggaggcccac actgccaaga
    27061 gtgggtgccg ccgacgacaa gaggtcgcag ccgtgactta aggcttggtt gacagccatg
    27121 gggtggcagc ccaaatttcg ccgccagtcg cttatattcg cctgcaaccg cgatgtttgg
    27181 gggctttatt gggggccttt taaaaaccgc actgaccaag aatggcggaa attaccaatt
    27241 tttacagatc agtgtggcgc acttcacccg tcgatcagct ctgcggcctt ccggtcatgc
    27301 gggaagggtg tggcgcaagt ggtcgtcagg atcgctcctg gcgcagcagc aggctggcgg
    27361 cggcacaggc cagcatgcac aggccagcga tcaggaaggc atcgctgtag gccagcagca
    27421 gcgcctgttc gtggaggttg ccggcaatca gcgcgagcac cttttggcgc agcggtgcgc
    27481 ccgccgcgcc atcaagcgcc gcgtgccggc ccggcgggtg cccggcgagc acgcctgcgg
    27541 ccagcgccat ccgctcgcgc aagggttccg accagggcgt taccgcctcg cccatgcggg
    27601 ccgcatggaa cttcgtccgc tccaccacca gctggctggc cagcgcgatg ccgatcgccc
    27661 cgcccacatt gcgcaaggtg gcaaacaggc ccgcggccga tcccgtctcg gccctggcca
    27721 tgccctgcat cgccatcacc cccagcgcca cgacgatgaa cgactgcccg atcccgcgca
    27781 ccaccagcga gggcacgatg acattggccg ccgcatcgcc atcgaggtgg atgttcatca
    27841 ggcagcccgc cgccatgagg aggaagccgg cgatgatgat ggtccgcacg ctggtccagc
    27901 gcatcaggcg cggcgtggca aacgacatga cgagctggac gaggccatag gggatcatcg
    27961 tcaggccgat ctgcgcggca ttgtagcctt gcgtctcggc gaaataattg ggcaccagaa
    28021 acaccacgcc aaagacaatg gcgccgaaca cgaactgcat caggctggca aggccgaacc
    28081 gggcctggcc cagcaggcgc aggttgatca gcggggccgg gcgccgcagt tcgataagcg
    28141 tgaaggcgat cagcgccgct gccgccacgc cggccgccgc gatgatgaag ccggaggcga
    28201 accagtcctg ccgctcgccc tcttccagca tgatctgcaa ggccgacagg cccagcgcca
    28261 tggtggcgat gccgctccag tcgccctcgc gcaggcggct gatccgcagg ggcgcgcgcg
    28321 gcatcgccca gccgatggcg gccagcagca gcagcccggg cggcacctgc agatagaaga
    28381 tccagcgcca gctgcaggcc tgggtcagcc agccccccag cgacggcccg gccgcctgcg
    28441 cgacattgtt cgagacagcg aacagcgcca tgccgaaagg atgtttttcc ggcggcagtt
    28501 cgctgacgat gatctgcagc gaaagcggga tcaacacccc gccaaaggcc ccctgcaagg
    28561 cgcgcgcggc gatcatggtg gcgatatccg gcgcgcagga gcacaggagg gagaacagca
    28621 cgaagccgcc cgtgcccacc atcagcacgc ggcgcatcga gaacaccgaa accagccagc
    28681 cggtcatcgg gatgatgatg atctcggcca ccagataggc cgtggtgatc cacgagccct
    28741 cctcgaagga ggcgcccagc gcgccgcgca catcgggcag cgaggcattg gtgacatgca
    28801 cgttcatgcc ggccatgaag cagcccacca cgccgcccag caccgctacc caggcccgca
    28861 gcgagacagc tgcgccggcg gttggcgcgc tcatggccgc gtgtccacgc tggtttccac
    28921 cgacatgccc ggccgcaatg cccccagccg cgcctgcccc gcatccagcc ggatgcgcag
    28981 gggcatgcgc tggacgatct tggtgaaatt gcccgtggca ttatcgggcg gcagcagcgc
    29041 gaattgcgcg cccgaggccg gcgcgaaact gtccacccgc cctgtcagca ccgccccgcc
    29101 aagggcatcg acggcgacgg tgaccggctg ccccgggcgg atgcgatcga tctgggtttc
    29161 cttgtaattg gccaccacat agggcgccat cggcaccagc gccagcagcg gcatgccgat
    29221 atcggcatat tggcccaggc gcagggcccg ctggccaacg atcccgctca ccggcgcgcg
    29281 aatggcggtg cgttccagat cgagccgcgc tgccgccagc ccggcacgcg cctgctgcag
    29341 ggccccgcgc gcgccggcgg ccgcggcgat ggcggcggcg cgttcggtag ccagcacctg
    29401 cacctgcgcc cgggctgcgg ccagcgcggc gcgggcctgt gtccatccgg cggcggcctt
    29461 gcggcgatcg gcctgggcgc tttgcagctt ctcctcgctg gtcacttgct ggcgggcgag
    29521 gtgggcctgg cgttctgcct gttgcgtggc cagctgcgcc tcggcctggc gggcggtcac
    29581 ggcggcggca ttctgggcga tggtgctgcg ctgctgctgc gcgcgcgcat cgagattggc
    29641 gatgcgcgct gtggccgcgg caaggccagc ctcggccacc gccagcgcgc cttgggcctg
    29701 cgccaccttc aggcgatagt cgcgcgggtc gatccgggcg aggattgcgc cgg
//
LOCUS       DASRYE010000106         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_93030,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000106 DASRYE010000000
VERSION     DASRYE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93030"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFF98_05865"
     CDS             <1..772
                     /locus_tag="VFF98_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63189.1"
                     /translation="VTLGLAALLFGLSRLGGPGGGGSVAGIGGWGWTGLGVLGLCLFA
                     MVERRQSDPIIPLALFATRARVAALASLALVAGSFMGVAVLMSFYIQQVLAYPPALAG
                     LALLPSTVGSFIGPALTGVLVPRIGARRVWMLGAASLLAGGAVLANLGPQSSYWTSVL
                     PGDFLAGLGLGLSLMPSSNILMYGVPHELNGAAGALNNATMQISAALGATAFNAIFVA
                     CGVAGGVRGYDLAFMAECAAAAVAIILIYSQVRDLPQA"
     gene            813..2213
                     /locus_tag="VFF98_05870"
     CDS             813..2213
                     /locus_tag="VFF98_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU63190.1"
                     /translation="MTFHRLNPITGEVASSAAAMQAADMPAMAATAAAAQPTWGAMGP
                     NARRAVLQQAADALAARKDDFLAAMMGEIGATAGWAMFNLGLAVSMVREAASLTTQIA
                     GEIIPSDKPGCLAMAVKEPVGVILGIAPWNAPIILGVRAIATPLACGNAVILKASEQC
                     PRTHALIIEAFAEAGFPEGLVQCVTNAPADAGDVVGALIDAPEVKRINFTGSTHVGRI
                     IARRAAEHLKPCLLELGGKAPFVVCADANLDEAVKAAAFGAYMNQGQICMSTERIIVV
                     DAVADAFAAKFAGKVKTLTAGDPRLGNTPLGGVVDAKTVAHCHSLIEDALAKGATLLT
                     GGETTLNVVMPAHLVDKVTQDMKLFRDESFGPVVGIIRARDEAHAIELANDTEYGLSA
                     AIFTTDIARGLTLARQIRSGICHINGATVHDEAQMPFGGVGASGYGRFGGRQGIDSFT
                     ETRWITVETQPGHFPI"
     gene            2251..3090
                     /locus_tag="VFF98_05875"
     CDS             2251..3090
                     /locus_tag="VFF98_05875"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HZU63191.1"
                     /translation="MTERAEADTVACTIENRIAWVRYNRPEKRNCMSPKLNRQMMRVL
                     EELEFREDVGVLVLSGEGSAFSAGMDLQEYFRDNEARGLGAIRQSQREAYGWWDRLRW
                     YEKPTIGMVNGWAFGGAYGPLHALDIVVAADEATFGLSEINWGILPGGGASKVAQELM
                     PFRKAIYHAMMGENLTGKQAAEQGLVTESVPAAELKARVTAIAEVLLKKDQNALRATK
                     WAMRRMSEMSFDNAMDYLIRAQEALNGMGGLAARKEATRQFLDEKTFKPGLGAFDTTK
                     IAK"
     gene            3152..>3970
                     /locus_tag="VFF98_05880"
     CDS             3152..>3970
                     /locus_tag="VFF98_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZU63192.1"
                     /translation="MHQPGHAMISRLLQPRSVAVVGASDKPGALGASLLGNLDRAGFA
                     GPVYPINPKRAHIGARACLASVEALPAGVDVAVLAIPRAFVLDTVKGLAQRQVGAAII
                     FAAGFAEEGEAGLAEQREIAAIAAEAGMVIEGPNCLGLVNHVDGVPLTFVETAIRKPA
                     GEAVSVVSQSGAMAAVLGTTLIARAVPLAYSVSTGNEAASGVEDYLDWMVDSPATRVI
                     ALIVEQFRSPGRFLAAARRAREAGKPIVLLHPGKSSAARASAATHTGAMAGDYSL"
BASE COUNT          631 a         1288 c         1410 g          641 t
ORIGIN      
        1 ggttaccctg gggctggccg cgctgctgtt cggcctgtcg cggctgggcg ggccgggggg
       61 cggcggcagc gtggcgggca tcggtggctg gggctggacg gggctgggcg tgctgggcct
      121 gtgcctgttt gccatggtcg agcggcggca gagcgatccg atcatcccgc tggccctttt
      181 tgccacgcgg gcgcgggtgg cggcgctggc cagccttgcc ctcgtggccg gctccttcat
      241 gggcgtggcg gtgctgatga gcttctacat ccagcaggtg ctggcctatc cgccggcgct
      301 ggccgggctg gcgctgctgc ccagcacggt cggctcgttc atcggcccgg cgctgaccgg
      361 cgtgctggtg ccgcgcatcg gcgcgcgccg ggtctggatg ctgggcgcgg ccagcttgtt
      421 ggcaggcggc gccgtgctcg ccaatctggg gccgcaatcg tcctactgga ccagtgtgct
      481 gcccggcgat tttctggccg ggctggggct ggggctcagc ctgatgcctt cgtcgaacat
      541 cctgatgtat ggtgttccgc atgaattgaa cggggccgcc ggcgcgctga acaacgccac
      601 catgcagatc agcgccgcgc tgggcgccac cgctttcaac gcgatcttcg tggcttgcgg
      661 cgtggccggg ggcgtgcgcg gatatgatct ggccttcatg gccgaatgtg ccgccgccgc
      721 ggtggcgatc atcctgattt attcgcaggt tcgcgatctg cctcaggcgt gaggcagggt
      781 tgctgatcgg cgattgctgc tgaggagcag gtatgacatt ccatcgatta aaccccatca
      841 ccggcgaggt ggcttcctct gccgccgcca tgcaggcggc cgatatgccc gccatggccg
      901 ccaccgccgc tgccgcccag cccacctggg gcgcgatggg gccgaacgcg cgccgcgccg
      961 tgctgcaaca ggccgccgat gcgctggctg ccaggaagga cgatttcctc gccgcgatga
     1021 tgggcgagat tggcgccacc gctggctggg ccatgttcaa cctgggcctt gcggtcagca
     1081 tggtgcgcga ggcggcgagc cttaccaccc agatcgccgg cgagatcatc cccagcgaca
     1141 agcccgggtg ccttgccatg gccgtaaagg agccggtggg tgtcattctg ggcatcgcgc
     1201 cgtggaacgc gccgatcatt ctgggcgtac gcgctatcgc cacgccgctg gcttgcggta
     1261 acgccgtgat cctgaaggcc agcgagcagt gcccgcgcac ccatgccctc atcatcgagg
     1321 cctttgccga ggccggcttc cccgaggggc tggtgcaatg cgtgaccaac gcgccggcag
     1381 acgcgggtga tgtggtgggc gcgctgatcg acgcgcctga agtgaagcgc atcaacttca
     1441 ccggcagcac ccatgttggc cggatcatcg ccaggcgcgc ggccgagcat ctcaagccct
     1501 gcctgctgga actgggcggc aaggcgccct tcgtggtctg cgccgatgcc aatctggacg
     1561 aggcggtgaa ggccgcggcc tttggcgcct atatgaacca gggccagatc tgcatgagca
     1621 ccgagcggat catcgtggtg gacgcggtgg ccgatgcctt tgccgccaaa ttcgccggga
     1681 aggtgaagac gctgaccgcc ggcgacccgc gcctgggcaa cacgccgctg ggcggggtgg
     1741 tcgatgccaa aaccgtcgcc cattgccata gcctgatcga ggacgcgctg gccaagggcg
     1801 cgactctgct gaccggcggc gagacgacgc tcaacgtggt gatgcccgcc catctggtcg
     1861 acaaggtgac gcaggacatg aagctgttcc gcgacgagag ctttggcccg gtcgtcggca
     1921 tcatccgcgc ccgcgacgag gctcatgcca tcgaactggc caacgacacc gaatatggcc
     1981 tctcggcggc gatcttcacc accgacatcg ccaggggcct gacgctggcc aggcagatca
     2041 gatcaggcat ctgccacatc aacggggcca ccgtgcatga cgaggcgcag atgcccttcg
     2101 gcggcgtggg cgcttcgggc tatggccgtt tcggcggcag gcagggcatc gacagcttca
     2161 ccgagacgcg ctggatcacg gtggaaaccc agcccggcca tttccccatc tgaccccatc
     2221 tcccccgaca ttccgcaaag gaccgacccc atgaccgaac gcgccgaagc cgacactgtt
     2281 gcctgtacga tcgagaaccg cattgcctgg gtgcgctaca accgcccgga aaagcgcaat
     2341 tgcatgagcc ccaagctcaa ccgccagatg atgcgcgtgc tggaggagct ggaatttcgc
     2401 gaggatgtgg gcgtgctggt gctgagcggc gagggcagtg ccttttccgc cggcatggac
     2461 ctgcaggaat atttccgcga caacgaggcc agggggctgg gcgccatccg ccagagccag
     2521 cgcgaggcct atggctggtg ggatcgcctg cgctggtatg aaaagcccac tatcggcatg
     2581 gtcaatggct gggcgtttgg cggcgcctat gggccgctcc atgcgctcga catcgtggtg
     2641 gcggctgacg aggccacctt tggcctctcg gaaatcaact ggggcatcct gcccggcggc
     2701 ggcgccagca aggtggcgca ggaactgatg cccttccgca aggcgatcta ccacgcgatg
     2761 atgggcgaga acctcaccgg caagcaagcc gccgagcagg gcctggtgac cgaaagcgtg
     2821 cccgccgccg agctgaaggc ccgcgtgacg gcaatcgccg aggtgctgct gaagaaggac
     2881 cagaacgccc tgcgcgccac caaatgggcc atgcgccgaa tgagcgagat gagcttcgac
     2941 aacgccatgg actacctcat ccgcgcgcag gaagcgctga acggcatggg cggccttgcc
     3001 gcgcgcaagg aagccacccg ccagttcctt gatgaaaaga ccttcaagcc cggccttggc
     3061 gccttcgata ccacaaaaat cgccaagtga accacaagcg cgagggccgc gccgccctcg
     3121 cgcgccccct ttcgagagag aatgcccgat catgcatcag cccggacacg ccatgatatc
     3181 acgcctgctt cagccgcgct cggtagcggt ggtgggcgcc agtgacaagc caggggcgct
     3241 gggggcctcg ctgctgggca atctggatcg ggcaggcttt gccgggcctg tctacccgat
     3301 caaccccaag cgggcgcaca tcggcgcgcg ggcctgcctt gccagcgtgg aggcgctgcc
     3361 cgcaggggtg gacgtggcgg tgctggcgat cccgcgcgcc ttcgtgctgg atacggtgaa
     3421 ggggctggcg cagcgccagg tgggggcggc gatcattttt gcggcgggct ttgccgagga
     3481 gggcgaggca ggccttgccg agcagcgcga gattgccgcc atcgccgcag aagcgggcat
     3541 ggtgatcgag gggccgaact gcctgggcct ggtcaaccat gtggacggcg tgccgctgac
     3601 cttcgtggaa accgccattc gcaagccggc gggcgaggcg gtgtcggtgg tgtcgcaatc
     3661 gggggcgatg gcggcggtgc tgggcaccac gctgatcgcg cgggcggtgc cgctggccta
     3721 ttcggtctcc accggcaatg aggcggcgag cggcgtggag gactatctcg actggatggt
     3781 ggacagcccc gcaacacggg tgatcgccct gatcgtcgag cagttccgca gcccggggcg
     3841 ctttctggcg gcggcgcggc gggcgcggga ggcgggcaag ccaatcgtgc tgctgcaccc
     3901 cggcaagagc agcgccgcgc gcgcatcggc ggccacccac accggcgcca tggcgggcga
     3961 ttacagcctg
//
LOCUS       DASRYE010000107        11719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_21987,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000107 DASRYE010000000
VERSION     DASRYE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21987"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFF98_05885"
     CDS             <1..118
                     /locus_tag="VFF98_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZU63193.1"
                     /translation="LHCSRIDGSPLRYNQPNTYMPDLLICRPEHAQHILSLI"
     gene            161..604
                     /locus_tag="VFF98_05890"
     CDS             161..604
                     /locus_tag="VFF98_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU63194.1"
                     /translation="MTENFASLVVQTARLLRRCFDERARRIGMTRPQWQVLSVLAKHE
                     GIHQSGLAEILEVEPMTVARMIDRLQEADLVERRPDPADRRAWRLHATARGLGLVDQL
                     KPLGEETLSLALDGVSEADHAAALRALEQIRANLLRRHTGAPGSD"
     gene            complement(615..1487)
                     /locus_tag="VFF98_05895"
     CDS             complement(615..1487)
                     /locus_tag="VFF98_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZU63195.1"
                     /translation="MTTAAFRPRRSALYLPASNPRAIAKAREVPADIIILDLEDAVAP
                     DQKDTAREAAVAAVYEGGFGHREVAIRCNGLDTPWGAADLAAVARSGADAVLVPKVAS
                     PACIQRYHDALAAAPEQTRLWAMIETCGSVLRLDAIGAMAASTRLSLWIMGTNDLAKE
                     MRARPAADRALFLPFLTMAVAAARAHGLAILDGVCNEFRDLAAFGAEAAQGAALGFDG
                     KSLIHPDQIAPANAVFAPSPADLAWADAVIAAFDLPENAGKGAIRVEGKMTELLHLEE
                     ARRLQAVAQAISAV"
     gene            complement(1702..2199)
                     /locus_tag="VFF98_05900"
     CDS             complement(1702..2199)
                     /locus_tag="VFF98_05900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZU63196.1"
                     /translation="MSDPHIDPALFRQVLGHYPTGVAAITSQGADGTPLALVVGTFTS
                     VSLDPPLVGFLPDKRSSSWPLIEATGHFAVNVLAADQGDLCRQLASAKGAEKFAGVEY
                     HLAEPGVPVLAGAPVAITCRIAWTHDAGDHVFVLGRVQALEVQREADPMLFHRGRYGS
                     FAAGN"
     gene            2467..3609
                     /locus_tag="VFF98_05905"
     CDS             2467..3609
                     /locus_tag="VFF98_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63197.1"
                     /translation="MTDLFQLNDDQLAIQEMARRFTADAITPFAARWDEEHAFPREVV
                     TAAGELGFGSIYVSEESGGIGLGRLEAALIVEAMAYGCPTTAAFISIHNMGAWMIDRF
                     GGAALKERYLPGAISMEKLVSYCLTEPGSGSDAAALKTTARLEGDHYVVNGTKQFISG
                     GGVNDLYVVMVRTGGEGPKGISTLVVEKDTPGVSFGAPEKKLGWNASPTAQVIFEEAR
                     IPVANRLGEEGDGFKFAMAGLDGGRINIGACSLGGAQRCLDEAVQYTKDRKQFGRAIA
                     EFQNTQFTLADMATDLEAARALLYLAAAKLSAGAADKTRFSAMAKKLATDNGSSIVDR
                     ALQLFGGYGYLRDYPIERFWRDLRVHRILEGTNEVMRLIIGRDLLK"
     gene            3626..4669
                     /locus_tag="VFF98_05910"
     CDS             3626..4669
                     /locus_tag="VFF98_05910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU63198.1"
                     /translation="MTDDVIIRREGVAGLLSLNRPKAIHALTHPMCEAMSAALSEWAA
                     DDAVQCIIIDHADGRGFCSGGDINLLRHSALSDEGASGRAFFAAEYRLNHQLFTYGKP
                     VVAFMDGITMGGGVGISQPARFRVATANTRYAMPETGIGLFPDVGGGWYLSRLRGHVG
                     SFLAVTGGRIDGADCVWAGLSTHYLDGEMLAGAKARIAGGEAIAAVLDELAEAPPPSK
                     LAAHAADIARHFGHATLEEILASLAADESAWAQKELAALRTKSPQACKVSLREIAESL
                     QNADFTAEMRMEYRIAARVLVLPDFAEGVRAVIVDKDNAPRWNPATPEGVSEAMIDAI
                     FAPLPAHEEWSPL"
     gene            4666..5448
                     /locus_tag="VFF98_05915"
     CDS             4666..5448
                     /locus_tag="VFF98_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU63199.1"
                     /translation="MNYETILVEQKGAVTLITLNRPQALNALNSTVLEELIAAFAAYE
                     ADESQLCAVITGSGEKAFAAGADIKEMAEKPAASFYKEDFFAGWTSRIVKAVRKPWIA
                     AVNGFALGGGCELAMMADFIIASENARFGQPEIKLGVAPGMGGSQRLTRAVGKAKAMD
                     MCLTGRMMDATEAERAGLVTRVVPLAELLADALKTAETIAAMPPLAAQMNKEMVNLAF
                     ETTLDQGLLAERRMFQVLTATEDKAEGMKAFVEKRPGVWKGR"
     gene            5473..6342
                     /gene="mmsB"
                     /locus_tag="VFF98_05920"
     CDS             5473..6342
                     /gene="mmsB"
                     /locus_tag="VFF98_05920"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HZU63200.1"
                     /translation="MKIGFIGLGNMGGGMAANLAKAGHGVQAFDLAEPALERARENGC
                     STFTTVREAVAGVDVVITMLPNGAIVNSVYTQDVFGHAPAGALLIDCSTIDVATTRTL
                     AEQAAAAGYTMVDAPVSGGIAAANGGTLTFMVGASEAGFAAVEPVLAPMAKAVIHAGP
                     NGSGQAVKMCNNMLLGATMIATCETFKLAERLGLDLQTFFDISSKATGQSWSMTSYCP
                     VPGVGPQSPADNGYQGGFASALMLKDLKLAMAAAEDAGAPVPMGRKAAELYAAFAEAG
                     EGGLDFSAIIKTL"
     gene            complement(6386..7507)
                     /locus_tag="VFF98_05925"
     CDS             complement(6386..7507)
                     /locus_tag="VFF98_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU63201.1"
                     /translation="MADMTGAMGDDSDNPAAESGHPAASAADKARKRYRVTSFDVAAE
                     AGVSQSTVSRALAGDPVVSEATRQRVYEAARKLNYHVDENAARLRTGRTGTLAVVVVS
                     RPGEDAKDFNPFHYTLLGNVCAAASAAGHETLVSFQDTEERLWGLYQEQRKADGLIVI
                     GTAENRPAWDYFRRIAGSGAHMVCWGSPFDELDWVRSDNAEGARLATGHLLAAGYRQI
                     VCVASETSAQRQFKERYDGYAARLAEEGLPARLLPFEEGFSRLEQGRRAGRLLLERRA
                     AEPFDAVFVCCDEMALGVLQSLREAGVQVPAQVGIVGFDGIRAGAHASPPLTSVEPDF
                     HAAGAMLVRRLLATINGQECAKERVPVQLLPRASSQRPA"
     gene            complement(7548..8345)
                     /locus_tag="VFF98_05930"
     CDS             complement(7548..8345)
                     /locus_tag="VFF98_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63202.1"
                     /translation="MAGGRAEGRLAGKRALITGAAGGLGAAMARAMAAEGARLLLGDV
                     NRAGAQALADEICAAQGSPVAIACRHDVTSPESWQAALALAREELGGLSVLVNNAGLG
                     TGGTIESTDLASWRQAFAVNVDGVFLGCQLALPLLRASTPAAIVNVGSVAGVVARSGM
                     APYGASKAAVLMLSRSVALHCAEAGWDVRCNCLMPGFADTPMIDGFRPAPGYPREKLV
                     DRLGAQVPLGRIARPEEIAQGAVFLASDESRYMTGTELKLDGGLSSR"
     gene            complement(8349..8894)
                     /locus_tag="VFF98_05935"
     CDS             complement(8349..8894)
                     /locus_tag="VFF98_05935"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZU63203.1"
                     /translation="MSAPEETMWQGDWITARRRGTWEYVGRARGIRAAVILAVDEANR
                     VILVEQFRVPLGRPCIELPAGLVGDEHEGEAPTAAAARELEEETGYRAARLEVVGEFW
                     SSPGMVTESFTLLRASGLEKVSAGGGVADEAITVHLVPIASLAAFLAERRALGDAIDM
                     KLMMLLGPAVLGLGAFAAQEG"
     gene            9083..9520
                     /gene="mscL"
                     /locus_tag="VFF98_05940"
     CDS             9083..9520
                     /gene="mscL"
                     /locus_tag="VFF98_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZU63204.1"
                     /translation="MLQEFKAFIARGNVLDLAVGVIIGAAFGKIVSSLTDDVLMPLIG
                     KITGGIDFSSKFILLGAIPASYTGSATDYAALKKAGVAMLGYGSFITAIINFLIMAFV
                     IFLIVKGANRLTARSKAEEAPAAPAGPTEVELLAEIRDALKGR"
     gene            9560..9961
                     /gene="ssrA"
                     /locus_tag="VFF98_05945"
     tmRNA           9560..9961
                     /gene="ssrA"
                     /locus_tag="VFF98_05945"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(10032..10268)
                     /locus_tag="VFF98_05950"
     CDS             complement(10032..10268)
                     /locus_tag="VFF98_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63205.1"
                     /translation="MFSHIARLFIIKTRLEAFAVIFALANGAVQRGSVYLTQFPGFGG
                     KLLFAACTAAVFMAGAKILDCLKHEQRRPEPGQG"
     gene            10532..>11719
                     /locus_tag="VFF98_05955"
     CDS             10532..>11719
                     /locus_tag="VFF98_05955"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011318.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HZU63206.1"
                     /translation="MSDLVRRSGLSVDAALARFVEEEVLAPLGRDVPAFWEGFAALVA
                     RLAPRNRALLDKREDLQAQIDAWHQARAGKVLDGAEYRAFLAEIGYLVPEPAPFAVTT
                     EHVDAEIATMAGPQLVVPVLNARFLLNAANARWGSLYDALYGTDALDAPAARAGGFDV
                     ARGAAVIASAKAFLDAAAPLEGLSWAEWDGLSADRPRPPLANPAQLVGRRGDGHKGAL
                     LLAHNGLHIEVVIDATHPIGATDRASIADVILESALTTIVDLEDSIAAVDAEDKLAAY
                     ANWLGVIRGDLADTFEKNGKTITRKLAADRAWTAPDGTAFTLPGRSLLFVRNVGHLMT
                     NPAILMADGAEIPEGIMDAVITSAISAQDLAGLGTYRNSREGSIYIVKPKMHGPEEVA
                     FAGE"
BASE COUNT         1928 a         3888 c         4031 g         1872 t
ORIGIN      
        1 cctccactgc agccgcatcg atggctcccc cctccgctac aaccagccca acacctacat
       61 gccagacctc ctcatctgca ggcccgaaca cgcacaacac atcctcagcc tcatctgacc
      121 gctggcgctg aggaaaaggg caagctatcg cggcagggcg atgacggaaa atttcgcttc
      181 cctggtggtg caaaccgccc gcctcctgcg ccgctgcttt gacgagcgcg cgcggcggat
      241 cggcatgacg cgcccgcaat ggcaggtgct gagcgtgctg gccaagcatg agggcatcca
      301 ccagagcggc cttgccgaga ttctggaagt ggagccgatg accgtggcgc ggatgatcga
      361 ccgcctgcag gaggccgatc tggtggagcg ccgccccgat ccggccgacc ggcgcgcctg
      421 gcgcctgcat gccacggccc gcgggctggg gctggtggat cagctgaagc cgctgggcga
      481 ggagacgctg agcctcgcgc tcgacggggt gagcgaggcg gaccatgccg ccgcgctgcg
      541 cgcgctggag cagatccgcg ccaacctgct gcgccggcac acaggggcgc cgggcagcga
      601 ttgagcagcg ccggttaaac cgctgagatc gcctgcgcca cggcctgcag gcgccgcgct
      661 tcttccagat gcagcagttc ggtcatcttg ccttccacgc ggatggcgcc cttgccggca
      721 ttctcgggca ggtcgaaagc ggcgatcacc gcatcggccc aggccagatc cgccggcgag
      781 ggggcaaaca ccgcattggc aggcgcaatc tggtcggggt ggatcaggct cttgccgtcg
      841 aagcccagcg ccgcgccctg cgctgcctcg gcgccgaagg cggccagatc gcgaaattcg
      901 ttgcacacgc catcgagaat ggcgaggcca tgggcgcggg cggcggccac cgccattgtc
      961 aggaagggca ggaagagcgc ccggtctgcc gccgggcggg cgcgcatttc cttggccaga
     1021 tcattggtgc ccatgatcca cagcgacagg cgggtggaag ccgccatggc gccgatcgca
     1081 tccagccgca gcacgctgcc gcaggtctcg atcatcgccc acaggcgggt ttgttcgggc
     1141 gctgcggcca gcgcgtcatg atagcgctgg atgcaggcgg gcgatgccac cttgggcacc
     1201 agcaccgcat ccgcgcccga gcgcgcgacc gcggcaagat ccgccgcgcc ccagggcgtg
     1261 tccagcccgt tgcagcgaat cgccacttcg cggtggccaa agccgccttc ataaacggcc
     1321 gccaccgcgg cctcgcgcgc ggtgtccttc tggtcgggcg ccacggcatc ctccagatcg
     1381 aggatgatga tatcggccgg cacctcgcgc gccttggcaa tggcgcgcgg gttggaggcg
     1441 ggcagataga gcgcgctgcg gcgcgggcgg aaagcggcgg tggtcatgcg cttgtctcct
     1501 ttggccgggg cgatggcccg ccgggcgcgc gccatcacct gcacaatgga tcaggattgt
     1561 gggcctgcca ggcaagcgcc cgccatgatc ccggtcaacc cccggggcgt tggcccgtgc
     1621 gtgggggccg gccagctgcc ggcttcagct gttggcagca aagctggcgt tttcagctcc
     1681 cggcagcaaa gctggcgttt ttcagttgcc ggcagcaaag ctgccgtagc gcccgcggtg
     1741 gaacagcatg ggatcggcct cgcgctgcac ttccagcgcc tgcacccggc ccagcacgaa
     1801 gacatggtcg ccggcatcat gcgtccaggc gatgcggcag gtgatcgcca ccggcgcgcc
     1861 cgccagcacc ggcacgccgg gctctgccag gtgatattcc accccggcga atttctccgc
     1921 tcccttggcg ctggccagct ggcggcacag gtcgccctgg tctgcggcca gcacattcac
     1981 cgcgaaatgg cccgtcgcct cgatcagcgg ccaggaggac gagcgcttgt cgggcaggaa
     2041 acccaccagc ggcggatcga gcgagaccga cgtgaacgtg cccaccacca gcgccagcgg
     2101 cgtgccatcc gcgccctgcg aggtgatggc ggcaacgccg gtgggatagt gccccagcac
     2161 ctggcggaag agggcggggt caatatgcgg atcgctcacg ttgttgtcct ggttgcgaaa
     2221 ggttggcccg gttgggtggg cgcaaggggg cggcccggcg ccggggtgcc agagcgggcg
     2281 gcttgctgca agcgcgttgt cgcgccatca tgggggcgat gggccggcgc tggctgcaca
     2341 aatgcaggcg tgagcggcgc aaaggcagct gtctcctagc aatctgccta cttgcattgc
     2401 gcgcccatgg gctgataccc gagcccttaa tcgatcgact aaataataac cgggatgccg
     2461 tctgccatga ccgacctgtt ccagctgaac gacgaccagc ttgccatcca ggagatggcc
     2521 cggcgcttca ccgccgatgc catcaccccc ttcgccgccc gctgggacga ggagcacgcc
     2581 ttcccccgcg aggtagtgac ggccgccggc gagctgggct ttggctccat ctatgtcagc
     2641 gaggaaagcg gcggcattgg ccttggccgg ctggaagcgg cgctgatcgt ggaggcgatg
     2701 gcctatggct gccccaccac cgccgccttc atctccatcc acaacatggg cgcctggatg
     2761 atcgaccgct tcggcggggc cgcgctgaag gagcgctatc tgcccggcgc catcagcatg
     2821 gaaaagctgg tgagctattg cctgacagag ccgggctcgg gctcggatgc ggcggcgctg
     2881 aagaccaccg cccggctgga gggcgaccat tatgtagtga acggcaccaa gcagttcatc
     2941 tcgggcggcg gcgtgaatga cctttatgtg gtgatggtgc gcaccggcgg cgagggcccc
     3001 aagggcatca gcacgctggt ggtggaaaag gacacgcccg gcgtctcctt cggcgcgccg
     3061 gaaaagaagc tgggctggaa tgcctcgccc accgcgcagg tgatcttcga ggaggcgcgc
     3121 atccccgtgg ccaaccggct gggcgaggag ggcgacgggt tcaaattcgc catggccggg
     3181 ctggatggcg ggcgcatcaa catcggcgcc tgctcgctgg gcggcgccca gcgctgcctc
     3241 gacgaggcgg tgcaatacac caaggaccgc aagcagttcg gccgcgccat cgccgaattc
     3301 cagaacaccc agttcaccct ggccgatatg gcgaccgacc tcgaagcggc ccgcgcgctg
     3361 ctctatctgg ccgccgccaa gctgagcgcg ggggcggcgg acaagacgcg tttttccgcc
     3421 atggccaaga agctggcgac cgacaatggc agcagcatcg tcgaccgggc gctgcaattg
     3481 ttcggtggtt acggctattt gcgcgattat ccgatcgagc gcttctggcg cgatctgcgc
     3541 gtgcatcgta tccttgaagg caccaatgag gtgatgcgcc tcatcatcgg ccgcgacctg
     3601 ttgaaataag cgagagaagc cagacatgac cgacgatgtg atcatccgcc gcgaaggggt
     3661 ggcgggcctg ctctcgctga accgccccaa ggcgatccat gcgctgaccc acccgatgtg
     3721 cgaggcgatg agcgcggcgc tgagcgaatg ggcggcagat gatgccgtgc aatgcatcat
     3781 catcgaccat gcagacgggc gcggcttctg ttccggcggc gatatcaacc tgctgcgcca
     3841 ttccgcgctg agcgacgagg gcgcctcggg ccgggccttc ttcgccgcgg aatacaggct
     3901 gaaccaccag ctgttcacct atggcaagcc ggtggtggcc tttatggatg gcatcaccat
     3961 gggcggcggc gttggcattt cgcagccggc gcgcttccgc gtggccaccg ccaacacccg
     4021 ctatgccatg ccggaaaccg gcattggcct gttccccgat gtgggcggcg gctggtatct
     4081 ctcgcgcctc aggggccatg tcggcagctt tctggcggtt acgggcgggc ggatcgatgg
     4141 cgccgattgc gtgtgggcgg gcctttccac ccattatctc gatggcgaga tgctggccgg
     4201 cgccaaggcg cgcatcgctg gcggcgaggc gattgccgcc gtgctggacg agctggccga
     4261 ggcgccgccg ccctccaagc tggccgccca tgccgccgat atcgcgcgcc atttcggcca
     4321 tgccacgctg gaggagatcc tcgccagcct ggccgcggac gagagcgcct gggcgcagaa
     4381 ggaactggcc gcgctgagga cgaaaagccc gcaggcctgc aaggtttccc tgcgcgagat
     4441 cgccgaaagc ctgcagaacg ccgatttcac cgccgaaatg cgcatggaat accgcattgc
     4501 cgcgcgcgtt ctggtgctgc ccgattttgc cgaaggggtg cgcgctgtca tcgtcgacaa
     4561 ggacaatgcc ccccgctgga accccgccac gcccgaaggt gtgagcgagg cgatgatcga
     4621 tgcgattttc gcgcccctgc cggcgcatga ggaatggagc ccgctatgaa ttatgaaacc
     4681 atcctggtgg agcagaaggg cgcggtcacg ctgatcacgc tgaaccggcc gcaggcgctg
     4741 aacgcgctga attccaccgt gctggaagag ctgatcgctg ccttcgccgc ttatgaggcc
     4801 gacgagagcc agctgtgcgc ggtgatcacc ggctctggcg agaaggcctt tgccgctggc
     4861 gccgacatca aggagatggc cgaaaagccg gccgccagct tctacaagga agatttcttc
     4921 gccggctgga cgagccgcat cgtcaaggcc gtgcgcaagc cgtggatcgc ggcggtgaac
     4981 ggctttgccc tgggcggcgg ctgcgaactg gcgatgatgg ccgatttcat catcgccagc
     5041 gaaaacgccc gcttcggcca gcccgagatc aagctgggcg tggcccccgg catgggcggc
     5101 agccagcgcc tgacgcgcgc ggtgggcaag gccaaggcga tggacatgtg cctgaccggc
     5161 cgcatgatgg atgccaccga agccgagcgt gcgggccttg tcacccgcgt ggtgccgctg
     5221 gccgagctgc tggccgatgc gctgaagacg gccgaaacca tcgccgccat gccgccgctg
     5281 gccgcccaga tgaacaagga aatggtcaac ctcgctttcg agacgacgct ggaccagggg
     5341 ctgctggccg agcgccgcat gttccaggtg ttgaccgcga cagaagacaa ggcggaaggc
     5401 atgaaggctt tcgtcgaaaa acgccccggc gtgtggaagg gccgctaagc ggcgattttc
     5461 gggagagatg agatgaagat cggctttatc ggccttggca acatgggcgg cggcatggct
     5521 gccaacctgg ccaaggccgg ccatggcgtg caggctttcg acctggccga gccggcgctg
     5581 gaacgcgccc gcgaaaatgg ctgcagcacc tttaccaccg tgcgcgaggc agtggcgggc
     5641 gtggatgtgg tgatcaccat gctgcccaat ggcgccatcg tgaacagcgt ctacacgcag
     5701 gatgtgtttg gccatgcacc ggccggcgcc ctgctgatcg attgctcgac gatcgatgtg
     5761 gccaccaccc gcaccctggc cgaacaggcg gccgcggccg gctacacgat ggtggatgcg
     5821 ccggtgtcgg gcggcattgc ggcggccaat ggcggcacgc tcaccttcat ggtgggggcg
     5881 agcgaggccg gctttgccgc ggtggagccg gtgctggcgc cgatggccaa ggcggtgatc
     5941 catgccggcc ccaacggttc gggccaggcg gtgaagatgt gtaacaacat gctgctgggc
     6001 gcgacgatga tcgccacctg cgagaccttc aagctggccg agcggctggg cctcgacctg
     6061 cagaccttct tcgacatctc gtcgaaggcg accgggcaga gctggtcgat gacctcctat
     6121 tgcccggtgc cgggggtggg gccgcaaagc ccggccgaca atggctatca gggcggcttt
     6181 gccagcgcgc tgatgctgaa ggacctgaag ctggcgatgg ccgccgccga ggatgcgggc
     6241 gcgccggtgc cgatgggccg caaggccgcc gaactttacg ccgcctttgc cgaagcgggc
     6301 gagggcgggc tggatttctc ggcgatcatc aagacgctgt gagccttgcg ggcggggcga
     6361 gggggaggcc cctttcgccc cgtcttcagg ccgggcgctg cgaactggcg cgcggcaaca
     6421 gctgcaccgg cacgcgctcc ttggcgcatt cctggccgtt gatggtggcc agcaggcggc
     6481 gcaccagcat ggcgccggca gcgtggaaat cgggctccac cgaggtgagc ggggggctgg
     6541 catgggcgcc ggcgcggatc ccgtcgaagc cgacgatgcc gacttgtgcg ggcacctgca
     6601 cgcctgcctc gcgcagcgat tgcagcacgc ccagcgccat ctcgtcgcag cacacgaaaa
     6661 ccgcgtcgaa cggttctgcg gcgcggcgtt ccagcagcag gcggccggcg cggcggccct
     6721 gttccagccg gctgaagcct tcctcaaagg gcagcaggcg ggcgggcagg ccttcctcgg
     6781 ccagccttgc ggcatagccg tcgtagcgtt ccttgaactg gcgctgggcc gaggtttccg
     6841 aggcgacgca gacgatctgc cgatagccgg ccgccagcag atggccggtg gccaggcgcg
     6901 cgccctcggc attgtccgat cgcacccagt ccagctcgtc aaacggcgag ccccagcaca
     6961 ccatatgcgc cccgctgccg gcgatgcggc ggaaataatc ccacgccggg cggttttcgg
     7021 cagtgccgat gacgatcagc ccatcggcct tgcgctgttc ctgatagagg ccccacaggc
     7081 gctcctcggt atcctggaag gagaccagcg tttcatgccc cgcggccgag gcggcggcac
     7141 atacattgcc cagcagcgtg tagtggaagg ggttgaaatc cttggcatcc tcgcccgggc
     7201 ggctgaccac caccacggcc agcgtgccgg tgcggccggt gcgcaggcgc gcggcgttct
     7261 cgtccacatg gtaattcagc ttgcgggcgg cttcataaac ccgctgacgc gttgcctcgc
     7321 tgaccaccgg atcgcccgcc agcgcccggc tgaccgtgga ttgcgacacg cccgcctcgg
     7381 ccgccacatc gaaggaggtg acgcgatagc gcttcctggc cttgtcggct gcgctggcgg
     7441 cgggatggcc gctttcggcc gccggattgt cgctgtcgtc ccccatcgcg cctgtcatgt
     7501 cagccattgc ggttgcttgt cctgtggccg gtcggcccat gcctgcgcta gcgggaagag
     7561 aggccgccgt ccagcttcag ctcggtgccg gtcatgtagc ggctttcgtc gctggcgagg
     7621 aagacggcgc cctgggcgat ctcttcgggc cgggcgatgc ggcccagcgg cacctgcgcg
     7681 cccagccggt ccaccagctt ttcgcgcgga tagcccgggg cggggcggaa accgtcgatc
     7741 atcggggtgt cggcaaagcc gggcatcagg cagttgcagc gcacatccca gcccgcctcg
     7801 gcgcagtgca gcgccaccga ccggctgagc atcagcaccg ccgccttgct ggcgccatag
     7861 ggcgccatgc ccgaacgcgc caccacgccc gccaccgagc cgacattgac gatggccgca
     7921 ggcgtgctgg cgcgcagcag cggcagggcc agctggcagc ccaggaacac cccgtcgaca
     7981 ttgaccgcga aggcctgccg ccagctggcc agatcggtgc tctcgatcgt gccgccggtg
     8041 ccaaggccgg cattgttgac cagcaccgag aggccgccca gctcctcgcg cgccagggcc
     8101 agggcagcct gccagctttc cgggctggtg acatcgtggc ggcaggcgat cgccaccggg
     8161 ctgccctgtg cggcgcagat ctcgtcggcc agagcctgcg ccccggcgcg gttgacatcg
     8221 cccagcagca ggcgcgcgcc ctctgccgcc atcgcccggg ccatggccgc gcccagcccg
     8281 ccggccgccc ccgtgatcag cgcccgcttg cccgccagcc ggccttcggc gcgcccgccc
     8341 gccatggctc agccctcctg cgcggcaaag gcgcccaggc ccagcacggc cgggcccagc
     8401 agcatcatca gcttcatgtc gatggcatcg cccagcgccc ggcgctcggc cagaaaggcg
     8461 gcaaggctgg cgatgggcac cagatgcacg gtgattgcct catccgccac gccgccgccc
     8521 gcgctcacct tctccagccc ggaggcgcgc agcagggtga agctttccgt caccatgccc
     8581 ggcgaggacc agaattcgcc caccacctcc agccgggcgg cacgatagcc ggtttcctct
     8641 tccagctcgc gggcggcagc ggcggtgggg gcctcgccct catgttcgtc gcccaccagg
     8701 ccggcgggca gctcgatgca ggggcggccc agcggcacgc ggaactgctc gacgagaatc
     8761 accctgttcg cctcgtccac cgccaggatc acggcggcgc gaatgccgcg cgcgcggccc
     8821 acatattccc acgtgccgcg ccggcgcgcg gtgatccagt cgccctgcca catggtttct
     8881 tccggggcag acatgacagg cgggagcagg gcggggggaa gcggggcggc caagcggggt
     8941 ctcctcacag ggggggattt cgcgcctgcc atagcgcttt gtgcgctcgc agcaaagcat
     9001 ctggcatggg cagagggttt gcctttggcg cgcgctgggc taggcaggga gcagttgtga
     9061 aacgggcgca aagggacaag gcatgctgca ggaattcaag gccttcatcg cgcggggcaa
     9121 tgtgctggat ctggccgtgg gtgtgatcat tggcgccgct ttcggcaaga tcgtctcctc
     9181 gttgacggat gatgtgctga tgccgctgat cggcaagatc accgggggca tcgatttctc
     9241 ctccaaattc atcctgctgg gcgctattcc cgccagctac accggctctg ccacggatta
     9301 tgccgcgctg aagaaggcgg gcgtggcgat gctgggctat ggcagcttca tcaccgcgat
     9361 catcaatttc ctgatcatgg ctttcgtcat cttcctgatc gtgaagggcg ccaaccggct
     9421 gaccgcgcgc agcaaggccg aggaggcgcc tgccgcccct gccggcccca ccgaggtgga
     9481 actgctggcc gagatccgcg atgcgctgaa gggccgctga ggccctgctt cagagaggat
     9541 ggggaaaggg ggcatctgcc cccttttctt ttggcgcggg cgccctatat cgggcggtgc
     9601 cggctttggc cggatatggc gataaactgc ggcgtgcaat aggcgcagcg gacccggggg
     9661 cggtacccgg cggctccacc atcacctctt ccgctggcgg aagaaccctg ttgtcgcagg
     9721 ggtgttgatg gggccgaact aggatcgacg tgtgttgaaa agcgctgttt tcgtccgggc
     9781 tgagtaaccc gttaaaggct caaaactcat aagtgccaac gacaacgaag cacttgctct
     9841 ggctgcgtaa ttctaggacc tagcggtctg aagttacaaa tacagaacac ggttgaaccc
     9901 accgggtaac agaagggaat tcgggggccc ggggcgggcc tagcaacaga accgccccac
     9961 cctgctgcgg gacaggcttg ggccagaggc cccgttgctt ccataatggc cgggaatgcg
    10021 cgaggggggc gtcagccttg gccgggttct ggcctgcgct gttcatgctt caggcaatcg
    10081 aggatcttgg cgccggccat gaacaccgcc gccgtgcagg cggcaaacag cagcttgccg
    10141 ccaaagcccg gaaattgcgt gagatagacg ctgccgcgct gcaccgcgcc attggccagg
    10201 gcaaagatca ccgcaaaggc ttccagccgc gtcttgatga taaacaaccg ggcgatgtgg
    10261 ctgaacatac tcattttctg gcgcctttcc cggcagactt tagcgggggc gggcaggcgc
    10321 cggcaaggga tggataacgc aatgttaacc acaatccgtt ccaaatcggg atcgattgcc
    10381 ccagcggcct gccgtgcccc cccgcctgcc cgcagccggc ttgccaagct ggcgtggata
    10441 ggtcataagc ccttcattcc tcccccggag tctgccctta ttctgccgcg ctggccgcct
    10501 tggccggcgc cgctttatca tggtgagacg aatgagcgat ttggtgagac gttcggggtt
    10561 gtcggtcgat gcggcgctgg cccgctttgt cgaagaggag gttctggcgc ctctcggccg
    10621 cgatgtgccc gccttctggg agggctttgc cgcgctggtg gcgcgcctgg ccccgcgcaa
    10681 ccgcgccctg ctggacaagc gcgaggatct gcaggcgcag attgatgcct ggcaccaggc
    10741 ccgcgccggc aaggtgctgg atggcgcgga atatcgcgcc ttcctggccg aaatcggcta
    10801 tctcgtgccc gagcctgccc cctttgcggt gacgaccgag catgtggatg cggaaatcgc
    10861 caccatggcc gggccgcagc tggtggtgcc ggtgctcaat gcgcgcttcc tgctcaatgc
    10921 cgccaatgcc cgctggggca gcctctatga tgcgctgtat ggcaccgatg cgctggatgc
    10981 gccggcggcc cgcgcaggtg gcttcgatgt ggcgcgaggc gcggcggtta tcgccagcgc
    11041 caaggccttc ctcgatgccg cggcgccgct tgaagggctg agctgggcgg aatgggatgg
    11101 cctttcggcc gatcgcccgc gcccgccgct ggccaatccg gcgcagctgg tgggccggcg
    11161 cggcgatggc cacaagggcg cgctgctgct ggcccataat ggcctgcaca tcgaggtggt
    11221 gatcgatgcc acccacccga tcggcgcgac cgacagggcg agcattgccg atgtcatcct
    11281 cgaatcggcg ctgacgacga ttgtcgatct ggaagattcg atcgcggcgg tggatgccga
    11341 ggacaagctg gccgcctatg ccaactggct gggggtgatc cgcggcgatc tggccgacac
    11401 gttcgagaag aacggcaaga ccatcacccg caagctggcc gccgaccgcg cgtggacggc
    11461 gcccgatggc accgccttca ccctgcccgg gcgcagcctg ctgtttgtgc gcaatgtcgg
    11521 ccatctgatg accaatccgg cgatcctgat ggccgatggc gccgagatcc ccgaggggat
    11581 catggatgcc gtcatcacca gcgcgatcag cgcgcaggat ctggccgggc ttggcaccta
    11641 tcgcaacagc cgcgagggca gcatctatat cgtcaagccc aagatgcacg ggcctgagga
    11701 agtggccttt gccggcgag
//
LOCUS       DASRYE010000108        10930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_129935,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000108 DASRYE010000000
VERSION     DASRYE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129935"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /gene="xrtA"
                     /locus_tag="VFF98_05960"
     CDS             <1..1322
                     /gene="xrtA"
                     /locus_tag="VFF98_05960"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HZU63207.1"
                     /translation="RPSLAALRPQGWARGAALFAAAAGLWLVGRLSAVAVLAEAGAVA
                     MLAASVPFVLGRQVARALAFPLAYMVFLVPVGDEFIPALQTITARITVALVRASQIPA
                     HIDGVFIATPAGLFEIAQACSGAKFLVAMVALGVLAAHLCFAGWRRQVAFVAACAVVS
                     VLANGLRAFATVLIAQWVGAKAAGGIDHVIYGWVFFALVMGGLLAVAWRWFDRPSGPP
                     VLVVPKAGGRPWPVPLACAALMVLALLADLWAFAAEHLPAAMPDHIALPQVAGFAQVP
                     YAPRHPWSPRARGADHRLLGSYADAAGRRVDVFVALYAAQGTGAKPGGFGEGAWNGDQ
                     GWEWEANAAAPPAVRAERLLAADGTLRLAETSYRSGGLLTGSVVALRLATLADRLALR
                     PRAVAVLILSAEAAPGQEAAAHATMAALRRAMGPTGPWLDRVTGTQN"
     gene            1336..3234
                     /locus_tag="VFF98_05965"
     CDS             1336..3234
                     /locus_tag="VFF98_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HZU63208.1"
                     /translation="MCGIAGMFDLAGAARFGVAEVEAMCDAIAHRGPDGAGVWRADGV
                     ALGHRRLSIIDLAGSPQPMVAADGGAVIVFNGEIYNFRALRAELADLGARFHTDGDTE
                     VILAAWRQWGRACLDRLEGMFAFGLYDIASRSLFLARDRLGVKPLFYAALADGRVLFG
                     SELKALMAHPDLPRVVDPLAVEDYLCWGYVPDHRSILAGVRKLPAGHALLLQAGRGLP
                     APTRWWDVSFAERHKGSAEELGEELYRLMDEAVRARMVADVPLGAFLSGGVDSSSVVA
                     LMAGASAAPVKTCSIGFDVAALDETSYAADVARRFATDHRSRQVSAGDYAEISRLIAM
                     FDEPFADASALPTWRVCQLAREEVTVALSGDGADEALAGYRRHRFHAAEERLRGALPA
                     ALRGPLLGTLGRLYPKADWAPRPLRAKSTLLSLATSGEEAYAQALSVTRADLRARLYT
                     PDFARLRGDYRAEDALVALMRGAPARSGLDRAQYADLAFWLPGDILTKTDRASMAVGL
                     EAREPLLDHRLIAFAAGLPEDMRIRAGQGKWLMKETMRRQLPEQILFRPKQGFVTPIA
                     QWFRGPLAEQARGIARASGLGRTGWLDGPRIAAIAEAHIAGRADHSRLLWQLVMLDGA
                     LAHLGLAG"
     gene            3596..4609
                     /locus_tag="VFF98_05970"
     CDS             3596..4609
                     /locus_tag="VFF98_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZU63209.1"
                     /translation="MDRLSSALGHPRDDDAASSDRQAPPGGVERAAGDAALPAFALHG
                     GDEPLIERASQRLAVPEALLNLAQLDVLYDGDRRALWTFMRPTGRPAFNRDLLGDFES
                     WQALIGSHFGPAGLPLDFLLLGSRTPGVFCFGGDLALFQQLIRLRDEAALASYGHRCV
                     RILHRNFHALDLPMLTIGLVQGQALGGGFEALLSFDVLIAERGATFGLPEVMFGLFPG
                     MGAHSLLSRKVGTALADQIILSNRTYKAEDLYEMGLVAQLAEPGEGIATARAFMEKSG
                     RRLAGMVAAKRAMRLAAPVPLSEMEEIVNLWARAALQLTEGDLKLMSRLAATQARNYG
                     AAA"
     gene            complement(4647..6815)
                     /locus_tag="VFF98_05975"
     CDS             complement(4647..6815)
                     /locus_tag="VFF98_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63210.1"
                     /translation="MTPRSLLLSSTPLACALALGALPLAAHAADADADPAAPAAAQTP
                     PPIVVSARRLEAARDSIQPSLGADAYQLTNEAIKALPGGDNQPFSQIALQLPGVAQDG
                     FGQLHVRGDHANLQYRINGTILPEGLSMFGQTMSPRLINRFDLLTGALPAQYGLRTAG
                     VIDVTTKTGLFDNGGTISLYGGSHATLQPSIEYGGSSGATNYFVSASYTHNDLGIENV
                     NSAAHAIHDRTDQGQFFGYVEHIFSDSDKVSLLGGYSNQWFQIPNPDGLTSSGAYSYN
                     GRSAYLSNDLNETQLERTGFGQLAWLHDAGAITVQTAFFARYSSLAYRPDITGELLFN
                     GQAQQAYKSDVAVGLQSDLVWRASGHHTLRFGGYVQHDHSRSDTTTFVFPVDDAGNQS
                     GSAYGITDNAAQNAWMVSLYGQDEWKLTPRLTLNYGLRFDAYSAYRSEHQFSPRVNVV
                     WQPLHGTTLHAGYAHYFSPPPFELVANTTIARFAGTSAAAASTDDTTPFAERQQYFDA
                     GIQQQVARGLMLGMDGYYRISTNLIDEGQFGAPIILTPFNYQKGRIRGVNFYANYNRG
                     PWSAYANFAMSKAEGENIVSSQFSFGSDDLAYIRNHYIYLDHDQTYTGSAGVTYSFRH
                     GDLKGLKLGGSMIYGSGLRRDLVLADGSSVPNGDKLPAYEQFNLSTSYRLEKAGVELR
                     FDIINLFDARYEIRDGTGVGVGAPEWGPRRGFFFGISKDI"
     gene            complement(6934..8079)
                     /gene="zapE"
                     /locus_tag="VFF98_05980"
     CDS             complement(6934..8079)
                     /gene="zapE"
                     /locus_tag="VFF98_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZU63211.1"
                     /translation="MASAGPQPLPHPLPHRVLDRYRALVAAGELQEDAEQAAAAARLD
                     HLQQELEAPATGLLGKLFGKKAAAPRGLYMWGGVGRGKSMLMDLLHDCLAIPQKRRVH
                     FHAFMLEVHELMREIRKEEAGDPIPQLAERIARDLRLLAFDEMVVNNSADAMIMSRLF
                     TALIRERGVVIVTTSNRAPSELYKNGLNREHFLPFIALIEHELDVLTLNGPTDYRLER
                     LAGIDSWHSPLGNAATQRVREVFFRLTDYPPEDAAHVPSADLDLGGRLLHVPKSLKGV
                     AVFSFKRLCGEARGASDYLAVARAYHTVILVGIPRMGPEQRNEAARFVTLIDALYEHK
                     VKLFVTADAEPEDLYEAGDGRFEFDRTISRLTEMQSRDYMALGHGED"
     gene            complement(8072..8491)
                     /locus_tag="VFF98_05985"
     CDS             complement(8072..8491)
                     /locus_tag="VFF98_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63212.1"
                     /translation="MAAAGALQAADPAPAAPATGATKPASAALHKIYVRNVCPRPVQV
                     LIDNAEVEHTWQPHAWFQLKANEATYLTGPDGAKLSQLADHQLYFYAETTDSAEAMRW
                     HGDGPEVQWQNMIYRTARITPVIDKDGDFVLRLTCNG"
     gene            complement(8600..9382)
                     /locus_tag="VFF98_05990"
     CDS             complement(8600..9382)
                     /locus_tag="VFF98_05990"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZU63213.1"
                     /translation="MATFTLPANSKISGKGRVHKAEGAGRVKKFTVYRYDPDSGENPR
                     YDSFEVDLDKCGPMVLDALLKIKNEVDSSLTLRRSCREGICGSCAMNINGRNGLACTT
                     AIEDLKGDVRITPLPHMEVIKDLVPDFTHFYAQYASIRPWLQTVSTTPSGKERLQSPE
                     QREKLDGLYECILCACCSTSCPSYWWNSDKFLGPAILLQAYRWLADSRDEMTGERLDE
                     LEDPFRLYRCHTIMNCANVCPKGLSPARAIAEIKKMQAERHV"
     gene            9530..10495
                     /locus_tag="VFF98_05995"
     CDS             9530..10495
                     /locus_tag="VFF98_05995"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZU63214.1"
                     /translation="MSLRPASDSVAGMKAPPPAALPGPPARLPRSMLAMAVKGGELSV
                     RAKGQPGGLGQDVYHRLMRLSWPRLLGLFMGVFLGFNLVFAVAYWLDPAGLAVAHEDA
                     GLPPYWRDFFFSVHTVATIGYGNIYPADLYANVIVVIEITLGIVYFALATGMAFARFS
                     RPTARMLFSQVLVVHDVAGVPTLMLRAANQRHNMIYSAEARMAVLLDGEVGGVRMRRF
                     VDLPLVRATNPAFALTWTIMHTIDAASPLHPWLASQAEGMDQDIVVILWGVDEVSGQT
                     IHGRWAYGPGDIRWDARFEDIVDVDEGGIRTIDYTRFHATHRMPA"
     gene            complement(10565..>10930)
                     /locus_tag="VFF98_06000"
     CDS             complement(10565..>10930)
                     /locus_tag="VFF98_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZU63215.1"
                     /translation="EAPAEAPVVEAAPEAPAEEPAPKPKRTRRKKAEVAAEAPAETPV
                     VEAAPEAPAEEPAPKPKRTRKKKVEAPAEAVTEAPAAPAVEEAPAPTPASAAEAAEGE
                     GEDGEDGPRKGWWQRTFGA"
BASE COUNT         1620 a         3473 c         3861 g         1976 t
ORIGIN      
        1 ggcggcccag ccttgcggcc ctgcggccgc agggctgggc ccggggggcg gcgctgtttg
       61 cggcggcggc gggcctgtgg ctggtgggcc ggctgtcggc cgtggcggtg ctggctgagg
      121 cgggggcagt ggccatgctg gccgccagtg tgcccttcgt gctgggccgg caggtggcgc
      181 gggcgctggc ttttccgctg gcctatatgg tgtttctggt gccggtgggc gatgaattca
      241 tccctgcgct gcaaacgatc actgcccgca tcaccgtggc gctggtgcgc gccagccaga
      301 tccctgccca tatcgatggc gtgtttatcg ccacgccagc cggcctgttc gagattgccc
      361 aggcctgttc tggcgccaag tttctggtgg cgatggtggc attgggcgtg ctggcggcgc
      421 atctgtgctt tgccggatgg cgccggcagg tggcgttcgt ggcggcttgt gcggtggtgt
      481 cggtgctggc caatggcctg cgcgcctttg ccacggtgct gatcgcccag tgggtggggg
      541 caaaggcggc cggcggcatc gatcatgtga tctatggctg ggtgttcttc gcgctggtga
      601 tgggcgggtt gctggcggtg gcctggcgct ggtttgatcg gccttcgggg ccgccggtgc
      661 tggtggtgcc gaaggccgga ggcaggccat ggccggtgcc gctggcctgt gccgcgctga
      721 tggtgctggc gctgctggca gacctgtggg cctttgccgc cgagcatctg cccgctgcca
      781 tgcccgatca catcgccctg ccgcaggtgg caggctttgc gcaggtgccc tatgcgccgc
      841 gccacccgtg gagcccgcgc gcgcgcggcg ccgatcatcg cttgctgggc agctatgccg
      901 atgcggccgg gcggcgggtg gatgtgttcg ttgcccttta tgccgcgcaa ggcacgggcg
      961 ccaagcccgg cggctttggc gaaggcgcgt ggaatggcga tcagggctgg gaatgggaag
     1021 ccaatgccgc tgccccgccc gctgtgcggg ccgagcgcct gctggccgcc gatggcacct
     1081 tgcgcctggc cgaaaccagc tatcgcagtg gcggcctgct caccggcagc gtggtggccc
     1141 tgcgcctggc cacgctggca gacaggctgg ccctgcgccc gcgggcggtg gccgtgctca
     1201 tcctttcggc agaagcggcg cccggccagg aagccgccgc ccatgccacc atggcggcct
     1261 tgcgccgcgc gatggggccc accggcccct ggctcgaccg ggtgaccggc acgcagaatt
     1321 gaagggactg aacgcatgtg cggcatagcc ggtatgttcg atctggcagg cgcggcgcgc
     1381 tttggcgtgg ccgaggtgga ggccatgtgc gatgcgattg cccatcgggg ccccgatggc
     1441 gccggcgtgt ggcgggccga tggggtggcg ctgggccacc ggcgcctgtc gatcatcgat
     1501 ctggccggat cgccccagcc gatggtggcc gcagatggcg gggcggtgat cgtcttcaac
     1561 ggcgagatct acaatttccg cgcgttgcgg gcagagctgg ccgatctggg cgcgcgcttc
     1621 cacaccgatg gcgataccga agtgatcctg gccgcctggc gccagtgggg ccgcgcctgc
     1681 ctcgatcggc tggagggcat gtttgccttc gggctctacg atatcgccag ccgctcgctg
     1741 tttctggcgc gggatcggct gggggtgaag ccgctgttct atgccgccct cgccgatggg
     1801 cgggtgctgt tcggatcgga actgaaggcg ctgatggccc atcccgatct gccgcgcgtc
     1861 gtcgatccgc tggcggtgga ggattatctg tgctggggct atgtgcccga tcaccgctcg
     1921 atcctggccg gcgtgcgcaa gctgccggcc ggccatgcgc tgctgctgca ggcgggccgg
     1981 gggctgcctg cgcccacgcg ctggtgggat gtctcctttg ccgagcggca caagggcagc
     2041 gccgaggaac tgggcgagga gctttaccgc ctgatggacg aggccgtgcg cgcgcgcatg
     2101 gtggccgatg tgccgctggg cgccttcctc tcgggcgggg tggacagttc cagcgtggtg
     2161 gcgctgatgg cgggcgcttc ggcggcgccg gtgaaaacct gctcgatcgg cttcgatgtc
     2221 gcagcgctcg atgaaacctc ctatgccgcc gatgtcgccc ggcgctttgc caccgatcat
     2281 cgcagccgcc aggtttcggc cggcgattat gccgagatct cccgcctcat cgccatgttc
     2341 gacgagccct ttgccgatgc ctctgccctg ccgacatggc gcgtgtgcca gctggcgcgc
     2401 gaggaagtga cggtggcgct gtcgggcgat ggcgcggatg aggcgctggc cggctatcgc
     2461 cgccaccgct tccatgccgc cgaggagcgc ctgcgcggcg ccctgccggc ggcgctgcgc
     2521 gggcccttgc tgggcacgct ggggcggctt taccccaagg cggattgggc gccgcgcccg
     2581 ctgcgggcca aatccaccct cctgtcgctg gccaccagcg gcgaggaggc ctatgcgcag
     2641 gcgctctcgg tcacccgggc ggatctgcgc gcccggctct acacgcctga ttttgcccgg
     2701 ctgcgcggcg attatcgcgc cgaggacgcg ctggtggcgc tgatgcgcgg cgcgccggcg
     2761 cggtccgggc tggaccgggc gcaatatgcc gacctcgcct tctggctgcc cggcgatatc
     2821 ctcaccaaga ccgatcgcgc cagcatggcc gtggggctgg aagcgcgcga gccgctgctc
     2881 gaccaccggc tgatcgcctt tgccgccggc ctgcccgagg atatgcgcat ccgcgccggc
     2941 cagggcaaat ggctgatgaa ggagaccatg cgccggcaat tgccagagca gatcctgttc
     3001 cgccccaagc agggtttcgt cacgccaatc gcccaatggt tccgcggccc gctggcagag
     3061 caggcgcgcg gcattgcccg cgcttcgggc ctggggcgca cgggctggct ggatggcccc
     3121 cgcatcgccg ccatcgccga ggcgcatatc gccgggcggg cggatcattc gcggctgctg
     3181 tggcagctgg tgatgctgga tggcgcgctg gctcatctgg ggctggcggg ctgagccaca
     3241 cctccggtag accgggcctg cagtaacgcc caggcggcac gctgtgcggg cctttatctg
     3301 atgcggcaat ggcggttggc gtgtcggaaa atcgccgtcg cttctgtgtt attctttaca
     3361 actggcgccg cgccagccct gtacagaaaa cccggaaaag cgccattttc aggaattaac
     3421 agcgatattt caggtatatg ccgcagtggc gagtgcccca ttgcgggacg gagatggttt
     3481 acaaaacgta aaaaacgctt tgcatgtaat tgcgtaaatg gtttaaaaac catgatgtta
     3541 gcaagcgggg cggcaaaaag ggccgaattc cggcaatgct acaggaggcc cggatatgga
     3601 tcggctgtcg agtgcccttg gccacccccg cgacgacgat gcggcgtctt ccgatcggca
     3661 agcgccgccg ggcggcgtgg aacgggccgc cggcgatgcc gcgcttcccg cctttgccct
     3721 gcatggtggc gacgagccgc tgatcgagcg ggccagccag cgcctcgccg tgcccgaggc
     3781 gctgctcaac cttgcccagc tcgatgtgct gtatgatggc gaccggcgcg cgctgtggac
     3841 cttcatgcgc cccaccggcc ggcccgcctt caaccgcgat ctgctgggcg attttgaaag
     3901 ctggcaggcg ttgatcggca gccatttcgg cccggccggc ctgccgctgg atttcctgct
     3961 gctgggcagc cgcacgcccg gcgtgttctg ctttggcggc gatctggcgc tgttccagca
     4021 gctgatccgg ctgcgtgacg aggcggcgct ggcttcctat ggccaccgct gcgtgcgcat
     4081 tctccatcgc aatttccacg cgctcgacct gccgatgctg accatcggcc tggtgcaggg
     4141 ccaggcgctg ggcggcggct tcgaggcgct gctctccttc gatgtgctga tcgccgagcg
     4201 gggggccacc tttggcctgc ccgaagtgat gttcggcctg ttccccggca tgggcgccca
     4261 ttcgctgctg tcgcgcaagg tgggcacggc gctggcggac cagatcatcc tctcgaaccg
     4321 cacctacaag gcggaagatc tgtacgagat ggggctggtg gcccagctgg cagagccggg
     4381 cgaggggatc gccacggcgc gcgccttcat ggaaaaatcg ggccggcgcc tggccggcat
     4441 ggtggccgcc aagcgcgcca tgcgcctggc agcgccggtg ccgctttccg agatggagga
     4501 gatcgtcaac ctgtgggccc gcgccgcgct gcagctcacc gagggcgatc tcaagctgat
     4561 gtcgcgcctg gccgccaccc aggcccgcaa ttacggcgct gcggcctgaa cggggcggaa
     4621 agcacgcttc cgccccggcg gcctgctcag atatccttgc tgatgccgaa gaagaagccc
     4681 cggcgcggcc cccattccgg cgcgcccacg cccacgcccg tgccatcgcg gatttcgtag
     4741 cgcgcgtcga acaggttgat gatgtcaaag cgcagctcca ccccggcctt ttccagccga
     4801 tagctggtgg aaaggttgaa ctgctcatag gcgggcagct tgtcgccatt gggcacgctg
     4861 ctgccatcgg ccagcaccag atcgcgccgc aggcccgagc catagatcat gctgcccccc
     4921 agcttcagcc ccttcagatc gccatggcgg aagctgtagg tgacgccggc tgagccggtg
     4981 taggtctggt cgtggtcgag atagatgtag tggttgcgga tataggcgag atcatccgat
     5041 ccgaagctga actggctgga gacgatattc tcgccctcgg ccttcgacat ggcgaaattg
     5101 gcataggccg accagggccc gcgattgtaa ttcgcataga aattgacgcc gcggatgcgc
     5161 cccttctgat agttgaaggg ggtgaggatg atcggcgcgc cgaactggcc ctcgtcgatc
     5221 agattggtgg agatgcggta gtagccgtcc atgcccagca tcagccccct ggcgacctgc
     5281 tgctggatgc cggcatcgaa atattgctgg cgttcggcga agggggtggt gtcatcggtg
     5341 ctggcggcag cggcgctggt gccggcaaag cgggcgatgg tggtgttggc caccagctcg
     5401 aagggcggcg gcgagaagta atgggcatag ccggcatgca gggtggtgcc atgcagcggc
     5461 tgccacacca cattgacgcg cggcgagaac tggtgctcgc tgcgataggc cgaataggca
     5521 tcgaagcgca ggccataatt cagcgtcagc ctgggcgtca gcttccattc gtcctggcca
     5581 tagaggctga ccatccaggc gttttgcgcg gcattgtcgg tgatgccata ggcgctgccg
     5641 gactggttgc cggcatcgtc caccgggaag acgaaagtgg tggtgtcgct gcgcgaatga
     5701 tcgtgctgca catagccgcc aaagcgcagc gtgtgatggc cgctggcccg ccacaccaga
     5761 tcgctttgca ggcccacggc cacatcgctc ttgtaggcct gctgggcctg gccgttgaac
     5821 agcagctcgc cggtgatatc gggccggtag gccagcgagg aatagcgggc gaagaaggcc
     5881 gtctgcacgg tgatggcgcc ggcatcatgc agccaggcca gctggccaaa gccggtgcgc
     5941 tccagctgcg tctcgttgag gtcgttcgac agataggcgg agcggccgtt gtagctatag
     6001 gcgccagaac tcgtcagccc gtcgggattg gggatctgga accactggtt ggaatagcca
     6061 cccagcaggc tcaccttgtc gctgtccgag aagatatgct cgacatagcc gaagaactgc
     6121 ccctgatcgg tgcgatcatg gatggcatgg gccgcgctgt tgacgttctc gatacccaga
     6181 tcattgtggg tgtagctggc cgagacgaaa tagttggtgg cgcccgaaga gccgccatat
     6241 tcgatgctgg gctgcagcgt ggcatggctg ccgccataca gcgagatggt gccgccattg
     6301 tcgaacaggc cggtcttggt ggtgacatcg atcacgccgg cggtgcgcag gccatattgg
     6361 gcgggcaggg cgccggtcag caggtcgaag cggttgatga ggcgcgggct catcgtctgg
     6421 ccgaacatcg acaggccctc gggcaggatg gtgccgttga tgcgatattg caggttggca
     6481 tgatcgccgc gcacatgcag ctggccaaag ccatcctgcg ccacgccggg caattgcagg
     6541 gcgatctggc tgaagggctg gttgtcgccg ccgggcagcg ccttgatcgc ctcgttggtc
     6601 agctgatagg catcggcgcc aaggctgggc tggatactgt cacgcgcggc ctccagccgg
     6661 cgggcggaga cgacgatggg cggcggcgtt tgggccgcgg cgggggcggc gggatcggca
     6721 tccgcatcgg ccgcatgggc ggccagcggc agggcgccaa gcgccagcgc gcaggccagg
     6781 ggcgtggagg agaggagaag ggagcggggc gtcatggtgt ctcgcttgcc agagaagatg
     6841 cgctgctctt atatgggatg atacaacgta tcaaggggcc aatgtgattt tgtatcatca
     6901 tccagccggc ccaacactcg tctcgtcgga aagctagtcc tcgccgtggc ccagggccat
     6961 gtagtcgcgg ctctgcatct cggtcagccg gctgatcgtg cggtcgaatt cgaagcggcc
     7021 atcgccggcc tcatagaggt cttccggctc tgcatcggcg gtaacgaaca gcttcacctt
     7081 gtgctcgtag agcgcgtcga tcagcgtgac gaagcgggct gcctcattgc gctgttcggg
     7141 ccccatgcgc ggaatgccca ccaggatcac cgtgtgatag gcccgcgcca cggcgagata
     7201 atcgctggcc ccgcgcgcct cgccgcacag ccgcttgaag ctgaacaccg ccacgccctt
     7261 caggcttttg ggcacatgca gcaggcgccc gcccagatcg agatcggccg agggcacatg
     7321 cgctgcatct tccggcggat agtcggtcag gcggaagaac acctcgcgca cgcgctgggt
     7381 ggcggcattg cccagcgggc tgtgccagct gtcgatgccg gccaggcgct ccagccggta
     7441 gtcggtgggg ccgttcagcg tcagcacgtc cagctcatgc tcgatcagcg cgatgaaggg
     7501 caggaaatgc tcgcggttca gcccgttctt gtaaagttcg gaaggggcgc ggttgctggt
     7561 ggtcacgatc accacgccgc gctcgcggat cagcgcggtg aacagccggc tcatgatcat
     7621 cgcatcggcc gaattgttga cgaccatttc gtcgaaagcc agcaggcgca ggtcccgggc
     7681 gatgcgctcg gccagctggg ggatgggatc gcccgcttcc tccttgcgga tctcgcgcat
     7741 cagctcgtgc acttccagca tgaaggcatg gaaatgcacg cgccgcttct gggggatggc
     7801 caggcaatca tgcagcaggt ccatcagcat ggacttgccc cggccgacgc cgccccacat
     7861 gtaaagcccg cggggcgcgg ccgccttctt gccgaacagc ttgccaagaa ggcccgtggc
     7921 cggcgcctcc agctcctgct gcagatggtc gagccgggcg gcggcggcgg cctgctcggc
     7981 atcttcctgc aactcgccgg cggccaccag cgcgcgatag cgatcgagca cgcggtgggg
     8041 caggggatgg ggcagcggct gcggcccggc actagccatt acaggtgagg cgcagcacga
     8101 aatcgccatc cttgtcgatc accggggtga tcctggcggt gcgatagatc atgttctgcc
     8161 actgcacttc gggcccgtcg ccatgccagc gcatggcctc ggcgctgtcg gttgtctcgg
     8221 catagaaata gagctggtga tcagccagct ggctcagctt ggcgccatcg gggccggtga
     8281 gataggtggc ctcattggcc ttcagctgga accaggcatg gggctgccag gtgtgttcca
     8341 cctcggcatt gtcgatcagc acctgcaccg ggcgcgggca gacattgcgc acatagatct
     8401 tgtgcagcgc tgcagaggcc ggctttgtgg ccccggttgc gggcgcagcc ggggccggat
     8461 cggcggcctg cagggcgccg gccgcagcca tcagcccggc gccaaaagcg agaagaagat
     8521 agccgcgcaa cccgtttcct ccgccggcag gggcggaaag cggcagacag cgcctgccgc
     8581 ttcgccccgc cgcttgtcct cagacgtggc gctcggcctg catcttcttg atttcggcaa
     8641 tggcgcgcgc ggggctgagg cccttggggc acacattggc gcagttcatg atggtgtggc
     8701 agcgatagag gcggaaggga tcttccagct cgtcgaggcg ctcgccggtc atctcgtcgc
     8761 ggctgtcggc cagccagcga taggcctgca gcaggatcgc cgggcccagg aacttgtcgg
     8821 aattccacca gtagctgggg cacgaggtgg agcagcaggc gcacaggatg cactcgtaga
     8881 ggccatcgag cttctcgcgc tgttccgggc tctgcagccg ctccttgccc gaaggcgtgg
     8941 tcgaaaccgt ctgcagccag gggcggatcg aggcatattg cgcatagaaa tgggtgaaat
     9001 caggcaccag gtccttgatc acttccatat gcggcagcgg ggtgatgcgc acatcgccct
     9061 tcagatcctc gatcgcggtg gtgcaggcca ggccgttgcg gccgttgatg ttcatcgcgc
     9121 aggaaccgca gatgccctcg cggcacgaac ggcgcagggt gaggctcgaa tcaacctcgt
     9181 tcttgatctt cagcaatgca tcgagcacca tcggcccgca cttgtcgaga tcaacctcga
     9241 agctgtcgta gcgcgggttc tcgcccgagt ccgggtcgta gcgatagacg gtgaacttct
     9301 tcacgcgccc ggccccctcg gccttgtgca cacggccttt gccggagatc ttgctgttgg
     9361 caggaagggt aaaagtggcc atatccgctg ctttccgctg tgaaatccag gggcaggcgg
     9421 cttgctggca aaccgccgac tctgcagccc tcttagcgtc ttggcgcgcg ggggcaagca
     9481 ccctgttgca ttgcggcgga atggataggc gcagagatgc gcaaaggatt tgagcctgcg
     9541 ccccgccagc gatagcgtgg ccggcatgaa agcgccccct cccgccgcct tgccgggccc
     9601 gccggcccgc ctgccccgat ccatgctggc catggcggtg aaggggggcg aattgtcggt
     9661 gcgggccaag gggcagccgg gcgggctggg gcaggatgtc taccaccggc tgatgcgctt
     9721 gtcgtggccg cggctgctgg gcctgttcat gggcgtgttc ctgggcttca accttgtctt
     9781 cgccgtggcc tactggctgg atccggccgg gcttgccgtg gcgcatgagg atgccggcct
     9841 gccgccctac tggcgcgatt tcttcttcag cgtgcacacg gtggccacca tcggctatgg
     9901 caacatctat ccggccgatc tttatgccaa tgtcatcgtg gtgatcgaga tcacgctggg
     9961 catcgtctat ttcgcgctgg ccacgggcat ggcctttgcc cgcttttcgc gccccaccgc
    10021 gcgcatgctg ttcagccagg tgctggtggt gcatgatgtg gcaggcgtgc ccaccttgat
    10081 gctgcgcgcg gccaatcagc gccacaacat gatctattcg gccgaggcgc gcatggcggt
    10141 gctgctggat ggcgaggtgg gcggcgtgcg catgcgccgc tttgtcgatc tgccgctggt
    10201 gcgcgccacc aacccggcct tcgccctgac ctggacgatc atgcacacga tcgacgcggc
    10261 cagcccgctg cacccctggc tggccagcca ggccgagggg atggaccagg atatcgtcgt
    10321 gatcctgtgg ggtgtcgacg aagtgagcgg gcagacgatc catggccgct gggcctatgg
    10381 gccgggcgat atccgctggg atgcccgctt cgaggatatc gtcgacgtcg acgaaggcgg
    10441 catccgtacg atcgactaca cccgcttcca cgccacccac cgcatgcccg cctgaggcgc
    10501 agcaaaacgg gcggcctcca ccaggagacc gcccgttcca ggctatctgc ctttcagcgc
    10561 cggatcaggc gccgaaagtg cgctgccacc agcccttgcg ggggccgtct tcgccatcct
    10621 cgccctcgcc ttcggccgct tcggcggcag aggcgggggt aggggcaggg gcttcttcca
    10681 cggccggggc ggcaggcgcc tcggtcacgg cttcggcggg ggcctccacc ttcttcttgc
    10741 gggtgcgctt gggcttgggc gccggctctt cagccggtgc ttcaggcgca gcttccacaa
    10801 ccggggtttc agccggcgct tcggcggcca cttcggcctt cttgcggcgg gtgcgcttgg
    10861 gcttgggcgc cggctcttcc gccggagctt caggcgcagc ttccacaacc ggggcttcag
    10921 ccggcgcttc
//
LOCUS       DASRYE010000109         6645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_112476,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000109 DASRYE010000000
VERSION     DASRYE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112476"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFF98_06005"
     CDS             <1..435
                     /locus_tag="VFF98_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HZU63216.1"
                     /translation="PAYLARHGTPATPDDLASHACLTFTFADGERRWRFTQGERLAQI
                     RVKGPLRANQAEALKEAALAGFGLVMLPTWLVGEEIAQGLLHPVLAPWHAEIGRPGMA
                     PAQEGAIHALYLPDRKGSPKVRAFVDFLAERFGAPPYWDRAL"
     gene            563..1699
                     /locus_tag="VFF98_06010"
     CDS             563..1699
                     /locus_tag="VFF98_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZU63217.1"
                     /translation="MTDLLFAPTRIGALNLPSRIVMAPLTRSRAAAGNVPSPLAVDYY
                     RQRASAALIITEGAQISPQGQGYAWTPGIHSQAQIDGWARIARAVHQAGGRIVIQLWH
                     VGRVSHSVFQPGGGRPVAPTAMPVPAKAFIPGADGQGTFADVPEPQELTIAGIQMIIA
                     DYVQAARNAIAAGLDGVEVHAANGYLLDQFLNSASNWRTDRYGGSPENRARLLLEVVD
                     AVCAAIGSQRVGVRLAPMGKAFGMDEAQPEALFHHVASELDKRDLAYLHLVEPTVKGR
                     EIAAEADPRAEALMRDIRARFAGPIILAGGYTRRNAEQALADGRGDLIAFGRPFLANP
                     DLPARLRIDAPLNPADPATFYGGGAHGYTDYPTLAEQAAGKLTA"
     gene            1696..2880
                     /locus_tag="VFF98_06015"
     CDS             1696..2880
                     /locus_tag="VFF98_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63218.1"
                     /translation="MTAPASRPMGPRFTFAMAATTGLAVANIYYNQPMLGLIERDLPG
                     AAAGFVPTITQLGYALGLFLLVPLGDLVERRRLICGQFLALAVALAAAALAPGGSALL
                     AGSLLVGALATVAQQIVPLAAHMAPPARRGAVIGQVMAGLLLGILLSRTLAGFVAQQA
                     GWRSMFWLGVPLAVAAGGVLRVLLPVSRPEPGPSYPALLASLWHLWRELPVLRRATCT
                     QALLFAAFSAFWSILALHVQRLGHGPSVAGLFGVVGAAGVFAAPLAGHLADRRGPTAT
                     ITAGTVLAMAGWAIAGLSQGLAGLAIGCIVLDLAVQGALISHQHAVYALRPEARARLN
                     TLFMGGMFLGGALGSALAMAAWLAAGWHGVGLLGSGLAALALVFHHCRGARAGLAATA
                     MG"
     gene            2917..3555
                     /locus_tag="VFF98_06020"
     CDS             2917..3555
                     /locus_tag="VFF98_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63219.1"
                     /translation="MEYIPEMASKQTVYQARGLERRKQLIRACRALLGERHVGQFGLA
                     EVAAVAGMPKTSAYHFFPRMEDLLAALAVDIAQDLLAYLNRPIVGPFAAWSQVVGAFV
                     DRGRAFYGQSRAALEIQLGPHTPADIKNRDRLNDVSVGICLKRHIKQFFIVPNLPSLD
                     DAFFRSIEIADLMFMLSVREHDGLTDFYVEEAARAACAYLELYLPRMLPAVD"
     gene            3625..5586
                     /locus_tag="VFF98_06025"
     CDS             3625..5586
                     /locus_tag="VFF98_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZU63220.1"
                     /translation="MSEPEDATGQPGMDVTRRDFVGGTLLGSGAALLAMGAPGALTGT
                     AHAQTTGLPMTGLGPDWSGPGGVGDYAGLNGNTYKVVNAAHGAIRNQMMDPKLAAATE
                     TGEVYDVVIVGAGISGLCAAYVIRSQRPDAKILLLDQHGIFGGEAKANEFEVDGYHLQ
                     GPQGSTGIVVPFRHARAAGMYTHWARELGYPEDFTFQKAQNLSQPIKVPEDVWTPMNV
                     AWERADTAFYYEGKGMVKNPWRNGFDQAPISEKAKKALLQVEVFRTPPEREDWEQWLD
                     SMTYKQFLLDVVGVPADVIDEVTAYYDPIACCMGCGLGCDAISAYSAYNFLMPGTIGY
                     YRYQNDGADPTDTIYLATFPGGNAIAARKFLKMSNPDAIEGDDTLYGVWKGKVRWDQL
                     DRPGAPHRTRLNATVVEVRHEGRPDRASHARVTYLKDDKLFRVHARAVICAGQQHANK
                     HICRDISPEYREAMNAFQHASILTLNIALRNWRFLDRMGVACVRWFEGFGWWTGLRRN
                     LIVDGHETQPLDPNKPFVLTQYIPFPQPGVPFPQCCTNARMQLFSMSFADIEAATREQ
                     LTKMFGPYGFDWDRDVAGIVANRQGHAYFVGAPGFFFGKEGKPAPRQVLQKPFHRIAF
                     SHSELSGAQMWETAAEEGERAAKQVLALI"
     gene            5599..6102
                     /locus_tag="VFF98_06030"
     CDS             5599..6102
                     /locus_tag="VFF98_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63221.1"
                     /translation="MKSALLMAAALLCAPASAFAQASHSQIAPQNTAEMRAFKAAIRK
                     LYDEKERDFAQGRDDHILNRFYSGDAISFGEGEGISIGRADFKKLYDEVTRQSTVKVR
                     SVYTYVSGTAGWDWANFYVTPKDPAAKPFSFAILFVWAKEGGKWICKGDAFVKGAFAI
                     PAGGGAR"
     gene            6099..6458
                     /locus_tag="VFF98_06035"
     CDS             6099..6458
                     /locus_tag="VFF98_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HZU63222.1"
                     /translation="MKRLILVLALAGAQPAFAQDAAQGRAVFARCQACHVASRGAHAT
                     VGPNLFGVVSRKAGTQAGYDYSPAMKAAGFVWSVDKLDAYLKAPAKLVPGNKMPFFGL
                     PDPKDRLAVIAYLNTLH"
     gene            6472..>6645
                     /locus_tag="VFF98_06040"
     CDS             6472..>6645
                     /locus_tag="VFF98_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HZU63223.1"
                     /translation="MKTRAAVAFAPKTPLEIVEVDLEGPKAGEVLVEIMATGVCHTDA
                     YTLDGFDSEGIFPS"
BASE COUNT         1057 a         2152 c         2211 g         1225 t
ORIGIN      
        1 cccgcctatc tggcccgcca cggcacgccc gccacgccag acgatctggc cagccacgcc
       61 tgcctcacct tcacctttgc cgatggcgag cggcgctggc gtttcacgca gggcgaacgc
      121 ctcgcccaga tccgcgtcaa gggcccgctg cgcgccaatc aggccgaagc gctgaaggag
      181 gcggcgctgg caggcttcgg gctggtgatg ctgcccacat ggctggtggg cgaggagatc
      241 gcccagggcc tgctgcaccc ggtgctggcc ccctggcatg ccgagatcgg ccggcccggc
      301 atggcgcctg cgcaggaagg ggccattcat gccctctacc tgcccgatcg gaagggatcg
      361 cccaaggtgc gcgccttcgt cgattttctg gccgagcgct tcggcgcccc gccctattgg
      421 gaccgggccc tttaatcttt gcgaaatatg caaagatgat cgcgaggatg gcctgattat
      481 caaaatcctc aaacaggtgc agaccgccgg catcaacccg gacacagccc gcacggccct
      541 gtccttcaag gagtgccccc tgatgaccga cctgctcttc gcccccaccc gtatcggcgc
      601 gctcaatctg cccagccgca tcgtgatggc gccgctcacc cgctcgcgcg cggcggcggg
      661 caatgtgccc tcgccccttg ccgtggacta ttaccgccag cgcgcttccg ccgctctcat
      721 catcaccgaa ggcgcccaga tttccccgca aggccagggc tatgcctgga cgccgggcat
      781 ccacagccag gcccagatcg acggctgggc ccgcatcgcc cgggcggtgc atcaggcggg
      841 cgggcgcatc gtcatccagc tgtggcatgt cgggcgggtg tcgcacagcg tgttccagcc
      901 gggcggcggg cggcccgtgg cccccaccgc catgccggtg ccggccaagg ccttcatccc
      961 cggcgccgat ggccagggca cctttgccga tgtgcccgaa ccccaagagc tgaccatcgc
     1021 cggcatccag atgatcatcg ccgattatgt gcaggccgcc cgcaatgcca ttgccgccgg
     1081 gctggatggc gtggaagtgc atgccgccaa tggctatctg ctcgaccagt tcctcaacag
     1141 cgccagcaac tggcgcaccg accgctatgg cggctctccc gaaaaccgcg caaggctgct
     1201 gctggaagtg gtcgatgccg tgtgcgcggc catcggcagc cagcgggtgg gagtgcgcct
     1261 ggccccgatg ggcaaggctt tcggcatgga tgaagcgcag ccggaagcgc tgttccacca
     1321 tgtcgccagc gagctggaca agcgcgatct ggcctatctc catctggtgg agcccacagt
     1381 gaagggccgc gagatcgccg ccgaggccga tccgcgcgcc gaggcgctga tgcgcgatat
     1441 ccgcgcccgt tttgccgggc ccatcattct ggccggcggc tatacccgcc ggaacgccga
     1501 gcaggcgctg gccgatggcc gcggcgatct catcgccttt ggtcgcccct ttctggccaa
     1561 ccccgacctg cccgcccgcc tgcggatcga cgccccgctc aacccggccg atccggccac
     1621 cttctatggc ggcggcgccc atggctacac cgattacccc accctggccg aacaggccgc
     1681 aggaaagctg acagcatgac cgccccggcc agccgcccca tgggcccccg cttcaccttc
     1741 gccatggccg ccaccaccgg ccttgccgtg gccaacatct actacaacca gccgatgctg
     1801 ggcctgatcg agcgcgatct gccgggcgcg gcggccggct ttgtgcccac gataacgcag
     1861 ctgggctatg cgctgggcct gttcctgctg gtgccgctgg gcgatctggt ggaacgccgc
     1921 cggcttatct gcggccagtt cctggcgctg gccgtggcgc tggcggcagc ggcgctggcg
     1981 ccgggcggat cggcgctgct ggcaggctcg ctgctggtgg gggcgctggc cacggtggcc
     2041 cagcagatcg tgccgctggc cgcccatatg gcgccgcccg cccggcgcgg cgcggtgatc
     2101 ggccaggtga tggccgggct gctgctgggc attctgctca gccgcacgct ggccggcttt
     2161 gtggcgcagc aggcgggatg gcgcagcatg ttctggctgg gcgtgccgct ggcggtggcc
     2221 gctggcgggg tgctgcgggt gctgctgccg gtgtcgcggc cggagccggg gccttcctat
     2281 ccggccctgc tggcatcgct gtggcatctg tggcgcgagc tgccggtgct gcgccgcgcc
     2341 acctgcacgc aggccctgct gtttgccgcc ttcagcgcct tctggagcat ccttgccctg
     2401 catgtgcagc ggctgggcca tgggccgtcc gtggcggggc tgttcggggt ggtgggcgcg
     2461 gccggcgtat ttgccgctcc tctggccggc catctggctg accggcgcgg gcccaccgcc
     2521 accatcaccg ccggcaccgt gctggcgatg gccggctggg cgatcgccgg cctgtcgcag
     2581 gggctggcgg ggctggcgat cggctgcatc gtgctggatc tggccgtgca gggcgcgctg
     2641 atctcgcatc agcatgccgt ttatgcgctg cgccccgaag cccgcgcccg gctcaacacg
     2701 ctgttcatgg gcggcatgtt tctgggcggc gcgctggggt cggcactggc gatggcggcc
     2761 tggctggcgg ccggatggca tggcgtgggc ctgctgggca gcgggctggc ggctctggcg
     2821 ctggttttcc accattgccg cggcgcgcgc gccggccttg cggcaaccgc gatggggtag
     2881 ggcggcgggc gctcgggctt gcagcgcagg cgataattgg aatatattcc ggaaatggcg
     2941 agcaaacaga ctgtatatca ggcccgtggg ctggagcggc gcaagcagtt gatccgggcg
     3001 tgccgcgcgc ttctgggcga gcggcatgtc ggtcagttcg ggcttgccga agtggctgcg
     3061 gtggccggca tgcccaagac gtcggcctat cacttctttc cgcgcatgga agatttgctg
     3121 gcggccctgg cggtggatat cgcgcaggac ttgctggctt atctgaaccg gccaattgtg
     3181 gggccgttcg ctgcgtggtc gcaagtggtg ggcgcctttg tcgatcgcgg gcgggcgttc
     3241 tatgggcagt cgcgcgcggc gctggaaatc cagcttggcc cgcacacccc ggcagacatt
     3301 aaaaatcgcg acaggctgaa cgatgtgtct gtcggcatat gccttaaaag gcatataaaa
     3361 cagtttttta tcgttccaaa cctgccctcc ctcgacgatg cctttttccg ctcgatcgag
     3421 atcgccgacc tgatgttcat gctgtcggtg cgcgaacatg atggcttgac cgatttctat
     3481 gtggaggaag cggcgcgggc tgcctgcgcc tatttggagc tgtatctgcc gcgcatgctg
     3541 ccggcggtgg actgaccgct gtgcattgac attggtcgag tgacctataa tctcggaaaa
     3601 aattcgagtt atggagtcgt ctcaatgtcc gaaccggaag atgcaactgg ccagcccggc
     3661 atggatgtca cacgccgcga tttcgttggc ggcacattgc tgggatcggg cgcggcgctg
     3721 ctggcgatgg gcgcgccagg cgccctcacc ggcacggccc atgcccagac caccggcctg
     3781 ccgatgaccg gcctcgggcc ggactggtcg gggccggggg gcgtggggga ctatgccggc
     3841 cttaacggta acacctacaa ggtggtgaat gccgcccatg gggcgatccg caatcagatg
     3901 atggacccca agcttgccgc ggccacagaa accggcgagg tttatgacgt ggtgatcgtg
     3961 ggggcgggca tttcgggcct gtgcgccgcc tatgtcattc gttcccagcg gcccgatgca
     4021 aagatcctgc tgctcgatca gcatggcatc tttggcggcg aggccaaggc caatgaattc
     4081 gaggtggatg gctatcatct gcaggggccg cagggctcca ccggcatcgt cgtgcctttc
     4141 cgccacgcgc gcgctgcggg catgtatacc cactgggcca gggaactggg ctatccggaa
     4201 gatttcacct ttcagaaagc gcagaacctc tcgcagccga tcaaggtgcc ggaggatgtg
     4261 tggacaccga tgaatgtcgc ctgggaacgg gcggacacgg ccttctatta tgaaggcaag
     4321 ggcatggtga agaacccttg gcgcaatggt ttcgatcagg cgccgatatc ggaaaaggcc
     4381 aagaaggcgc tgctgcaggt ggaggtgttc cgcacgccgc cggagcgcga ggactgggag
     4441 cagtggctcg attccatgac atacaagcag tttctgctgg atgtggtggg cgtgccggcc
     4501 gatgtgatcg acgaggtgac ggcctattac gacccgattg cctgctgcat gggctgcggg
     4561 ctgggctgcg atgcgatctc tgcctattcg gcctataatt tcctgatgcc gggcacgatc
     4621 ggctattatc gctatcagaa tgatggcgca gaccccaccg acaccatcta tctggccact
     4681 ttccccggcg gcaatgcgat tgccgcgcgc aagttcctca agatgtcgaa ccccgatgcc
     4741 atcgaagggg atgacacgct gtatggcgtg tggaagggca aggtgcgctg ggatcagctg
     4801 gatcggcccg gcgcgcccca tcgaacccgg ctcaacgcca cggtggtgga agtgcgccat
     4861 gaaggccgcc ccgatcgtgc cagccacgcc cgggtgacct atctgaagga cgacaagctc
     4921 tttcgcgtgc atgcccgggc ggtgatctgc gccggccagc agcatgccaa caaacatatc
     4981 tgccgcgaca tttcccctga atatcgcgag gcgatgaatg ccttccagca tgcctcgatc
     5041 ctcacgctga acattgcgct gcgcaactgg cgcttcctcg acaggatggg ggttgcctgt
     5101 gtgcgctggt tcgaaggttt cggctggtgg acgggcctca ggcgcaacct catcgtcgat
     5161 ggccatgaaa cccagccgct cgatccgaac aagccctttg tgctcacgca gtatattccc
     5221 tttccgcagc ctggcgtgcc atttccccaa tgctgcacca atgcgcggat gcagctgttc
     5281 tcgatgtctt ttgccgatat cgaggcggcc accagggaac agctgacgaa gatgttcggc
     5341 ccctatggct ttgactggga ccgcgatgtg gccggcattg tcgccaaccg gcagggccat
     5401 gcctatttcg tcggcgcgcc gggcttcttc ttcggcaagg agggcaagcc ggcgccgcgc
     5461 caggtgctgc aaaagccctt ccaccgcatt gctttcagcc attccgaatt gtcgggcgcg
     5521 cagatgtggg aaaccgcagc cgaagagggc gagcgcgccg ccaagcaggt tcttgccttg
     5581 atctgaggag ttaattccat gaaatccgcc ctattgatgg ccgcggcgct gttgtgcgct
     5641 ccggccagcg ccttcgcgca agccagccat tcgcagatcg ccccgcagaa taccgccgag
     5701 atgcgcgcct tcaaggccgc catccgcaag ctgtatgacg agaaggagag ggatttcgcg
     5761 caagggcgcg acgatcatat cctcaaccgc ttctattccg gcgatgccat ctcgttcggt
     5821 gaaggcgagg ggatcagcat cggccgggcc gatttcaaga agctctatga cgaagtgacc
     5881 aggcagtcga ccgtcaaggt ccgctctgtc tacacctatg tgagcggcac tgccggctgg
     5941 gactgggcga atttctatgt gacccccaaa gaccccgctg ccaagccctt ctcctttgcg
     6001 atcctgttcg tctgggcgaa ggagggcggc aaatggatct gcaagggcga tgccttcgtg
     6061 aagggggcct ttgccattcc cgcgggggga ggcgcccggt gaagcgcctg atccttgtgc
     6121 tggcgctggc gggtgctcag cccgcctttg cccaggatgc ggcacagggc cgcgccgtct
     6181 ttgccagatg ccaggcctgc catgtggcca gcaggggcgc ccacgccacg gtgggcccca
     6241 atctgttcgg cgttgtgtcg cgcaaggccg gcacgcaggc gggctacgac tattctccgg
     6301 caatgaaggc ggcaggcttc gtctggtcgg tcgacaagct cgatgcctat ctcaaggccc
     6361 ccgccaaact ggtgccgggc aacaagatgc ccttcttcgg cctgcccgat cccaaggatc
     6421 gcctggcggt tatcgcttat ctcaacacat tgcattaaca ggggaataat catgaaaacc
     6481 cgcgctgccg tggcctttgc gcccaagacc cctctggaga tcgtggaggt ggatctggaa
     6541 gggccgaagg ccggcgaggt tctggtggag atcatggcga cgggcgtgtg ccacaccgat
     6601 gcctacacgc tggatggctt cgattcggaa gggattttcc cttcg
//
LOCUS       DASRYE010000110        20017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_200708,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000110 DASRYE010000000
VERSION     DASRYE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20017
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200708"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFF98_06045"
     CDS             complement(<1..575)
                     /locus_tag="VFF98_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZU63224.1"
                     /translation="MSPATIAAIAGRVRAAHSTARAEAAAALARADAYAAIQPAVWIS
                     RLPADQVLAMAAAVDARLAAGEDLPLAGVPFAIKDNIDLAGLATTAGCPAFAYRPDRS
                     ASVVERLLAAGAIPLGKANLDQFATGLNGTRSPYGAPACVTNLAYISGGSSSGSAVAV
                     AAGVVAFALGTDTAGSGRVPAAINGLIGLKP"
     gene            complement(572..1189)
                     /locus_tag="VFF98_06050"
     CDS             complement(572..1189)
                     /locus_tag="VFF98_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HZU63225.1"
                     /translation="MDIAAGPLVLDETVPARAPWMHGVARGQTLEIIDLEGNQAVDFL
                     LYAAADDAERYSAQDTVAMQGNIFLRKGSVLYSSEGNPMMRIVDTNVAYHDTIGGACS
                     CESNTLRYGHHTKAQHACVDNFLEANARAGRGKRDMVSNINWFMNVPVEGDGTLGIVD
                     GISAPGLSVRLEALMDVVAVVSNCPQINNPCNGFNPTAVRMIIRA"
     gene            complement(1196..2047)
                     /locus_tag="VFF98_06055"
     CDS             complement(1196..2047)
                     /locus_tag="VFF98_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HZU63226.1"
                     /translation="MQEGGAQPTGSSTGNPHAARDHARAQGGTRVEAMPVLPASAADG
                     WPQDVADADNVWAETLAAGGYAGKILARGTRLELTDLAGDGCVSLLAFNREMPTERLN
                     VADTVKIQWNGYLGEGRFLLTDMGRVLFSILADEAGTHDTFCGASNAAGNARKYGSGD
                     NYGPHPNARDRFRLGVAKFGLGVRDVHPCITLFKGARIGADGTIAPEIGPFAPGRRVV
                     LRAEMEVIVVLANCPHVLDPRPEYAVSPVRLRAWRGPVAGGDDALRNSTPEALRAFLN
                     TEDYYNR"
     gene            complement(2047..2700)
                     /locus_tag="VFF98_06060"
     CDS             complement(2047..2700)
                     /locus_tag="VFF98_06060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZU63227.1"
                     /translation="MSEAAKLARPMIVGQPVLIVVDVQKGSFAQWEASERLPMLPDRV
                     ERMERVRPLVDAARAAGVPVIFVKEEHRADMVDFGRELDGTENVHCLENSPYTPFAVE
                     ELGMRPDDYTITKRRYSCFFGTDLEILLRGLKAETLILVGGFTDVCVHYTYVDAHQHD
                     YYCRVVEPCTTASSPPAHVAALAAMEYLQKGAVRGLEEMIAGFAAYGAAAPAGQEQG"
     gene            3070..5433
                     /locus_tag="VFF98_06065"
     CDS             3070..5433
                     /locus_tag="VFF98_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63228.1"
                     /translation="MTKTLLQDRGRRRFASKSASYLLGASALLGTAGALLPTTAFAAS
                     AADNSAADNSAADTGAQALPEIVVSAQKRKETARNIPLSVSVFSAEKLEQDHVADFAD
                     LARATPGMAFTNTGNSSNTRISIRGISSSSGSSTVGIYLNDVSLTIPNQFFTGTTLPR
                     LFDLDHVEVLRGPQGTLYGDSSMGGTVKFVTRQPRLNTVEGTVYGGTGATEGGGVNYQ
                     FGGAINLPITQNVALRVAAETGYQSGWMSQVVNGVTEKKNANSERYTAVRATLLWEPA
                     PGLKITPALQYQYTSIGANSIFDLSQPAYTITKQVPEPSHDILMTPSLTIEKSFGDYT
                     LTSITAYTYRKYWRQFDVTSYDSAYVAGYIDPTYGSTYNTIANLPGTFWNADEVHNWS
                     EEVRFTSPSIKPHALSAEWQVGAYLNSLKTVSLDDEYVPGLNSQTEALTGDTVESLIG
                     YTAPSDQIGYFHSDRLLKQVAVFAEGSLMLTNRLKATVGIRQAWAWSSYLMNEGGWLA
                     DGTPAIDRAKSSNSPFTPKFALTYAVSPHASVYGNVSKGFRLGGQNNSLPSYCGSALS
                     SLGLSSDSAKSYGADSLWSYELGAKTGWLGNRLVLNGSLYRIDWHNLQQQINLTSCGY
                     VTTLNAGNARSQGAELELQARLTGTLSFNATASFTSARITQGASGLIAKAGQEVLAVP
                     NRSATFGLAYRKPIAARRSLFASFDWTYTGKSYGSYKATDSDYARPAYWVGDLDIGTE
                     YNGFTLRAFIKNIANDQTIIQRPQVLFISQGLTVQPRTFGVSLSRDF"
     gene            complement(5506..5718)
                     /locus_tag="VFF98_06070"
     CDS             complement(5506..5718)
                     /locus_tag="VFF98_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63229.1"
                     /translation="MTDRIDDDRQLEAALRACRDYLDSRRRVLARAYLGQCMEALESQ
                     KEPPRRKAFCGGDWARRRRQGMAAAA"
     gene            6077..6769
                     /locus_tag="VFF98_06075"
     CDS             6077..6769
                     /locus_tag="VFF98_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU63230.1"
                     /translation="MKIAILDDDPAHLDFATDVLCQAGHSCAPFSQGRQLIRSLHDEH
                     FDLFVLDWNLPDMSGLSVLQWIRNQIGSAPPVLLLTSRVADEDMVEALHAGADDYVSK
                     PCKAQVLQARVLALGRRIQPAGGERAVEGYGDYEFDLAARRVRHKGEQIDITPKEFQL
                     ALLLFRNVNIAVSRAYMLEAVWGMAPTAETRTADIHMSKLRRKMALGPANGYVLSPIY
                     GYGYRLEEISEA"
     gene            6805..8109
                     /locus_tag="VFF98_06080"
     CDS             6805..8109
                     /locus_tag="VFF98_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZU63231.1"
                     /translation="MALAPGMAHAGEPAVTRAEGPRPEIRYTVIHGDTLWHFAATWLV
                     GVSAADELQRLNHIPEPRHMPIGLVLRIPVDLLREEASVAVVEAFSGPVAILRGGHSE
                     PPRIGMQIGEGTGLATGANAFITLRLADRTAVSVPSQSSVSLARLRRQLLTGTLTRQV
                     DVGAGRLHATVTPMQDPASSFRVKTPLTVSAVRGTDFRIEFDDKGLAATTVSTGKVAV
                     GSGPAPRPEASALVPAGFGQATTPSGSSGLVKLLPAPELAAADKPQTGGTLAFAVAPV
                     AGAVQYRAQLARDAGMLDVVEESRAAAPLLRLPPVADGHYTLRLSAVDAQGLEGFTRT
                     YPVERQLRPVTLHMEQLGRGQRLAFRFGWTGGVAGASTWHFQLFRSQSGELPLIDRAA
                     IADAATEVPALPPGDYAWRVGEENGTDADWSPPQRFHIVAGR"
     gene            8112..10385
                     /locus_tag="VFF98_06085"
     CDS             8112..10385
                     /locus_tag="VFF98_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZU63232.1"
                     /translation="MPGADKAAARQLARQWWLALAGTAALVLLALAGHLTTRLDNAFY
                     DALIGRARQPADAGVLLVAIDERSLQQLGPWPWPRSLHARLLRQLAAGGAQAVALDIL
                     FVEPTRPEEDAALAQAMRAGPPAFLPVEFEVPGRDGAPFTLARPIPLLAEAAAGLGHV
                     NLAPDADGTTRRLYLAYQGQGHSWPQLASMLAGARGGTAAAGADLHGTAPVLLRYAGP
                     RGSFPTVSAASLLAGEVPPAMIAGKLVIIGATAAGLGDSYATPASADGSLMPGIEIQA
                     NLLATLRAGRGIAPAGWEAGLAFALAPLLLLMLALRLLPPRWMLVAAGLLVAGVVALS
                     AGLLLAGDLWLAPAPGLLGMALAYPLWTWRRLMAVSHYLSAELEKLDAESDPLERPRP
                     PLPEGDVVARQMGLLRSAIDRERDLRHFLVERIAQMPDAVLVCDLAGAVVLANQGACD
                     LAQALGAQWAGPGAHAAPLLACFTRTGEAPGPLDLPLAGLAEGWEGAVEAAGGRSFAL
                     RAEPQRNAENALVGQVLRIVETSEQVAMQRQREDVLQLLSHDMRAPQAAIIALLEQQG
                     AALPEGLAAQIAGHAGRTLAMADNFVHLSRAELMVFEPEPVDLCAIAQDAADALWPRA
                     RQRGVEVVTTLPDDELWVAGEASLLARALINLLDNAVKFAPAGATVNVTVAAAGGMAC
                     AHVANPGPPIPPAQMARLFERFTRAGSGPARKADGVGLGLAFVHRVATRLGGTIACTS
                     DAAQGTCFTLTLPRLPD"
     gene            complement(10400..12214)
                     /locus_tag="VFF98_06090"
     CDS             complement(10400..12214)
                     /locus_tag="VFF98_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZU63233.1"
                     /translation="MDRRSMIRGTAAGLAMAGLGGAALAGPEPQGAEGARLAALFDRI
                     AGEMLDRSPETVTSLGLDRGARAGAKARLDDRSLAAWQADKARNGAQLAALQAIDRAA
                     LAPRDRANLASVVFQLAVQDRLYRQLPYAGMPYAVSQLTGAYQQIPDFLDSQHSIASA
                     ADAEAYLARLAGFATALDQENEQVRHDAALGVVPPAFILATALQQLRALAGQGSDASN
                     LVQSLARRARAQAIAGDWEARAGAVYTGLVRPALARQIALLESLAPRAGAAAGVGHLP
                     QGGALYALSLQYYTTSTMPPAEIHREGLALVARLSGEADAILRRQGLSQGSVGARMRA
                     LYARPDQLYPNTEAGKAQLLADLAGLVARVEALLPAWFGARPRAHLAIRRVPREIEAG
                     APGGYYQPGALDGSRPGAYYINLRDTAEVPRWSLPTLTFHEGIPGHHLQLSLGQEAGL
                     PLIRKMQFFSGYGEGWALYAEQLAVEMGLYAGDPLGHLGQIHDALFRAVRLVVDSGLH
                     AQGWSRARAIAYYRAILGSPQAAAATEVDRYCVWPGQACSYMLGKLEWLRLREKARRA
                     LGPRFDIRAFHDAGLLPGAVPLPVLAGEIDAYIAANAA"
     gene            complement(12294..13325)
                     /locus_tag="VFF98_06095"
     CDS             complement(12294..13325)
                     /locus_tag="VFF98_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HZU63234.1"
                     /translation="MPKAQLAAFTDTLADFSYGAPAHAGSALRLTVSVFADRALLREE
                     ICEDAEGAGFRLGEVAPLAALHDGTASGLGDVVLVDCPAPDGAALAALARIDMRVAQA
                     GGELVVSTSVDGLDDVFGCLDQSAAQILVAPSRAERVIALGQVLARMPQRKVRELSDE
                     DRLVLLRLTEQVTQIAERIDRLGGPGGGPAALAAAATREGTVARLASTEAEAEAAAEG
                     DKYTRAPRPPLPDPRLVRRIIRQRQLRARYLDGDLFADPAWEMLLDLTAARAEHARVS
                     VTSLCIASGVPPTTALRWIGQMTESGLLQRMEDETDRRRAFITLTDKAADAMARYFAE
                     LGHNAARLV"
     gene            complement(13568..14857)
                     /locus_tag="VFF98_06100"
     CDS             complement(13568..14857)
                     /locus_tag="VFF98_06100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZU63235.1"
                     /translation="MANVTVIGAQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLV
                     VGEKVYKLSLLPSGIVTGTLSIIGNGVVLDPWALKAEVEKLEGQGVVITTENFAIAEN
                     CPLILPMHRDLDGLRETAAGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDALDPQ
                     LDRLCAHHDALRAGFGQPPVDRAALLEELRAIAPFVLQFAQPVWKRLNTVKKAGARIL
                     FEGAQGVLLDVDHGTYPFVTSSNTVSGTAASGSGLGPSATGFVLGIVKAYTTRVGSGP
                     FPTELEDETGQRLGERGHEFGTVTGRKRRCGWFDAVLTRQSCAISGVTGIALTKLDVL
                     DGFEKVKICTGYRLHGKVLDYYPAHAADQAAVEPIYEEMDGWQGTTAGARSWADLPAQ
                     AIKYIQRVQELIETPVALVSTSPEREDTILVRDPFAD"
     gene            complement(15019..16143)
                     /locus_tag="VFF98_06105"
     CDS             complement(15019..16143)
                     /locus_tag="VFF98_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZU63236.1"
                     /translation="MTDTTRDLLPEGLEDRLPQATAAATRITRALMDVFDAHGYDRVQ
                     PPSIEFEAPLASRMAGISPRRMFRFVDPASLGMLALRSDITPQVGRIAVTRLAGAARP
                     LRLAYGGQVLTIKGDGLDPTRERLQLGVELIGADSVAAAAEIVAVAVEALVAAGATGL
                     SVDFTLPDLVDTLAAGPLPLPAGAIDAVRAALDAKNASALVEAGGQAYLPLLYATGAF
                     EPALERLGQLPAGGALEGRIAALRQIAARVAGKARLTLDPTERHGFEYQSWFGFTLFA
                     EGLRGSVGRGGTYRILGAEGTAGEPATGFSLYPDPLIDALAAAEPARAALFLPLGHDE
                     AAAAALRAAGWRTVAALGEGDAAAALGCSHVLQGAKAVLA"
     gene            complement(16267..17859)
                     /gene="serA"
                     /locus_tag="VFF98_06110"
     CDS             complement(16267..17859)
                     /gene="serA"
                     /locus_tag="VFF98_06110"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZU63237.1"
                     /translation="MTETRKPRVLISDKMDPNAARIFTEMGCDVDVITGETPEQLIAR
                     IGDYDGLAIRSSTKVTKEILDAATNLKVIGRAGIGVDNVDIPYASAKGVVVMNTPFGN
                     SITTAEHAIAMIFALARQIPEANEQTQQGKWPKNGFMGVEVTGKVLGLIGAGNIGSIV
                     ASRALGLRMKVVAFDPFLTPERAVELGVEKVELDSLLARADFITLHTPLTADTKNILS
                     AENLAKTKKGVRIVNCARGGLIDEAALKEGLDSGHIAGAALDVFQTEPAKDSPLFGTK
                     NFICTPHLGASTTEAQVNVALQVAEQLGEYLTTGGVTNALNMPSLSAEEAPRLKPYMA
                     LAEKLGSLVGQLTPSAVARISIHTEGAAAELNAKPIVAAVLAGFMKVQSATVNMVNAP
                     FLAKERGLEVREVKTEKEGDYHTLIRVSVKTEAGERSVAGTLFNNREPRLVELFGIKV
                     EADLAGDMMYIVNEDAPGFIGRIGTLLGENAINIGTFNLGRREAGGEAILLLSVDNPV
                     GADVLDAAGKLPGVKRVKALAF"
     gene            complement(17948..19075)
                     /locus_tag="VFF98_06115"
     CDS             complement(17948..19075)
                     /locus_tag="VFF98_06115"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HZU63238.1"
                     /translation="MTVNAPATKPARPFFSSGPCAKPPVYDLAQLATESLGRSHRAKI
                     GKMRLAYCIDLMREILQLPETHRIGIVPGSDTGAFEMAMWTMLGARPVTTLAWESFGE
                     GWVTDVAKQLKLDPTILRADYGQIADLSAIDWSNDVLFTWNGTTSGVRVPNADFIPAD
                     REGLSFADATSAVFAYDIDWSKIDVATFSWQKVLGGEGGHGVLILGPRAVERLETYTP
                     AWPLPKVFRLMSKGKLAEGVFKGETINTPSMLAVEDAIFALEWARGLGGLEGLKARSD
                     ANAAALGKIVDERSWLSHLAADEAIRSKTSVCLSVEGADADFIKKFAAVLEKADAAYD
                     IAGYRDAPAGLRIWCGATVNVEDIEALGPWLDYAYETVKAG"
     gene            19292..19945
                     /locus_tag="VFF98_06120"
     CDS             19292..19945
                     /locus_tag="VFF98_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HZU63239.1"
                     /translation="MIPLILLPLGLLAGCLPQDQGHQPPRPRQQARAYAPSPAGAQCM
                     ADLHQRQAGFAPLPDAYYGGGCATINTVRLLSLHGDTDALAVSNLGPVTCPLADAFAG
                     WARYGVDRAARLALGSPLARIETMGSYNCRNIAGSDHRSAHATADAIDVSGFVLADGR
                     HISVLSQWSAGTAEERYFLRLVHTSACKRFGTVLGPDYNPAHRNHFHLELSAAGFCR"
BASE COUNT         3238 a         7102 c         6526 g         3151 t
ORIGIN      
        1 ctgggcttga ggccgatcag cccgttgatc gcggccggca cccggcccga tccggccgtg
       61 tctgtgccca gcgcgaaggc caccaccccc gctgccaccg ccaccgccga gcccgaggag
      121 gagccccccg aaatataggc cagattggtg acgcaggccg gcgcgccata ggggctgcgc
      181 gtgccattga gcccggtggc gaactgatcg agattggcct tgcccagcgg gatggcgccg
      241 gctgccagca agcgttccac cacgctggcc gagcgatcgg gccgataggc aaaggccggg
      301 cagcccgcag tggtggcaag gccggccagg tcgatattgt ccttgatggc aaagggcacg
      361 ccggccagcg gcagatcctc gccggcggcc agccgggcat ccaccgccgc cgccattgcc
      421 agcacctgat cggccggcag gcggctgatc cacaccgccg gctggatcgc cgcataggca
      481 tcggcccggg ccagcgcggc cgctgcctcg gcgcgggcgg tgctgtgtgc ggcccgcacc
      541 cgcccggcaa tggcggcaat cgtggcaggg ctcatgcgcg gatgatcatc cgcacggcgg
      601 tggggttgaa gccattgcac ggattgttga tctgcgggca gttcgagacc accgccacca
      661 catccatcag cgcctccagc cgcaccgaca ggcccggcgc cgaaatgcca tcgacaatgc
      721 ccagcgtgcc atcgccctcc accggcacat tcatgaacca gttgatattg gagaccatat
      781 cgcgcttgcc gcgcccggcc ctggcattgg cctcgaggaa attgtcgaca caggcgtgct
      841 gcgccttggt gtgatggcca tagcgcagcg tattggattc gcagctgcac gccccgccga
      901 tcgtgtcgtg ataggccacg ttggtatcga caatccgcat catcggattg ccctcgctgg
      961 aatagagcac gctgcccttg cgcaggaaga tgttgccctg catcgccacc gtatcctgcg
     1021 cgctgtaacg ctcggcatca tcggcggcgg catagagcag gaaatcgacc gcctggttgc
     1081 cttccagatc gatgatctcc agcgtttgcc cgcgcgccac gccatgcatc cagggcgcgc
     1141 gcgccggcac ggtttcatca aggacgagag ggcctgctgc aatatccatg gcggttcacc
     1201 tgttgtaata gtcttcggtg ttgaggaagg cgcgcagggc ctctggcgtg ctgttgcgca
     1261 aggcatcatc gccgccggcc accggcccgc gccaggcccg caggcgcacc gggctcaccg
     1321 catattccgg ccgcggatcg agcacatggg ggcaattggc cagcaccacg atcacctcca
     1381 tctcggcccg cagaaccacc cgccggcccg gcgcaaacgg cccgatctcc ggcgcgatcg
     1441 tgccatcggc gccgatccgc gcgcccttga acagggtgat gcaggggtgc acatccctca
     1501 cccccaggcc aaacttggcg acgcccaggc ggaacctgtc acgcgcgttg gggtgggggc
     1561 cgtaattgtc gccgctgcca tatttgcggg cattgccggc cgcgttggaa gcgccgcaga
     1621 acgtatcatg cgtgcctgcc tcatcggcca ggatggagaa gagcacccgg cccatgtcgg
     1681 tgagcaggaa gcggccttcg cccagatagc cgttccactg gatcttcacc gtatcggcca
     1741 cattcagccg ctctgtcggc atttcgcggt tgaaggccag caggctcacg cagccatcgc
     1801 cggcaaggtc ggtcagctcc agccgggtgc cgcgcgccag gatcttgccg gcatagccgc
     1861 ccgcggccag cgtttcggcc cacacattat cggcatcggc cacatcctgc ggccagccat
     1921 cggcagcgct ggccggcagc accggcatcg cctcaacccg ggtgccccct tgcgcccggg
     1981 catgatcgcg cgcggcatgg gggttgccgg tggaggaacc ggtgggctgg gcgccgcctt
     2041 cctgcatcag ccctgctcct gcccggcggg cgctgccgcg ccataggcgg caaagccggc
     2101 gatcatttcc tccagcccgc gcaccgcgcc cttttgcaga tattccatgg ctgccagcgc
     2161 cgccacatgg gccggcggcg aggaagcggt ggtgcagggc tccaccacgc ggcagtagta
     2221 atcgtgttga tgggcatcga cgtaggtgta atgcacgcac acatcggtga agccgcccac
     2281 caggatcagc gtttcggcct tcaggccgcg cagcaggatc tccagatcgg tgccgaagaa
     2341 gcaggaatag cgccgcttgg tgatcgtgta atcatccggc cgcatgccca gctcttccac
     2401 ggcaaagggc gtgtagggcg agttttccag gcaatgcacg ttttccgtgc catccagctc
     2461 gcggccaaaa tcgaccatgt cggcgcggtg ctcctccttc acgaagatca ccggcacacc
     2521 ggccgcgcgc gccgcatcaa ccagcgggcg cacccgttcc atccgttcca cccgatcggg
     2581 cagcatcggc aggcgctcgc tggcttccca ctgcgcaaaa gagcccttct gcacatcgac
     2641 cacgatcagc accggctgcc cgacgatcat cggcctggca agctttgcag cttcactcac
     2701 acattcgccc cttcagcatc tgtcatcgcc tttggatgca cgcgcgcggg cgagccgata
     2761 aaggcatggc tgcacaaatt cagttgttgg ggcgggcaac tattcccggc agcgaggcaa
     2821 tgcccgcagc ccttggttgt gcagccgcga aactaacttt gcctatgccg ccatttgaat
     2881 ctggccgctc tatgcaacgc tgagccatca aaacgcccgc ccccggcccg gatcggccgg
     2941 agaggcgatg gcaaaaaaca aaatcctcgc agggcggaca taaccgcaat gggcgatcgg
     3001 ccaggcaacg gcttgatcgc gccgagaaca cgcgcctacc aaaaggggcg ggaacatggg
     3061 gtattctgta tgaccaagac actgttgcaa gacaggggcc gccgccgttt cgccagcaaa
     3121 agcgccagct atctgctggg cgcgagcgcg ctgctgggca cggcaggcgc cttgctgccc
     3181 accaccgcct ttgccgccag tgcggccgac aattccgccg ccgataattc cgccgccgat
     3241 accggcgcgc aggccctgcc ggaaatcgtc gtctcggcgc aaaagcgcaa ggaaaccgcg
     3301 cgcaacatcc cgctcagcgt ctcggtgttc agcgccgaga agctggagca ggaccatgtc
     3361 gccgattttg ccgacctggc ccgcgccacc cccggcatgg ccttcaccaa caccggcaat
     3421 tcctcgaaca cgcgcatttc catccgtggc atttcctcct cttcgggctc gtccacggtg
     3481 ggcatctatc tgaacgatgt ctcgctcacc attcccaacc agttcttcac cggcaccacc
     3541 ctgccgcggc tgttcgatct ggatcatgtg gaagtgctgc gcgggccgca gggcacgctc
     3601 tatggcgaca gttcgatggg cggcacggtg aaattcgtca cccgccagcc caggctgaac
     3661 acggtggagg gcactgtcta tggcggcacc ggcgccaccg agggcggcgg cgtgaactac
     3721 cagttcggcg gggcgatcaa cctgcctatc acccagaacg tggcgctgcg cgtggcggcc
     3781 gaaaccggct atcagagcgg ctggatgagc caggtcgtca atggcgtgac ggaaaagaag
     3841 aacgccaact ccgagcgcta caccgccgtg cgcgctaccc tgctgtggga gccggcgccg
     3901 ggcctgaaga tcaccccggc gctgcagtat cagtatacca gcatcggcgc gaacagcatc
     3961 ttcgatctct cgcagcccgc ctacaccatc accaagcagg tgccagagcc cagccacgac
     4021 attctgatga ctcccagcct gacgatcgag aagagcttcg gcgattacac gctgacctcg
     4081 atcaccgcct acacctaccg caaatactgg cgccagttcg acgtcaccag ctatgattcg
     4141 gcctatgtcg ccggctatat cgatcccacc tacggctcca cctacaacac catcgccaac
     4201 ctgcccggca ccttctggaa cgccgacgag gtgcacaact ggagcgagga agtgcgcttc
     4261 acctcgccct cgatcaagcc gcatgcgcta tcggccgaat ggcaggtggg ggcctatctc
     4321 aactcgttga aaacggtgag cctggacgat gaatatgtgc ccggcctcaa cagccagacc
     4381 gaggcgctga ccggcgacac ggtcgaatcg ctgatcggct acaccgcgcc ctccgaccag
     4441 atcggctatt tccattccga tcggctgttg aagcaggtgg cggtgtttgc cgaaggatcg
     4501 ctgatgctga ccaaccggct gaaggccacc gtcggcatcc gccaggcctg ggcctggagc
     4561 agctatctga tgaacgaggg cggctggctg gccgatggca cccccgccat cgaccgcgcc
     4621 aaatccagca acagcccctt cacgcccaaa ttcgccctca cctatgccgt atcgccgcat
     4681 gcctcggttt atggcaatgt cagcaagggc ttccggctgg gcgggcagaa caattcgctg
     4741 ccctcctatt gcggcagcgc gctcagctcg ctggggctca gctcggacag cgccaaatcc
     4801 tatggggcgg attcgctgtg gtcgtatgaa ctgggcgcca agaccggctg gctgggcaac
     4861 cggctggtgc tgaacggatc gctctaccgg atcgactggc acaatctgca gcagcagatc
     4921 aacctcacct cctgcggcta tgtcaccacg ctgaacgccg gcaatgcccg cagccagggc
     4981 gcagagctgg aactgcaggc caggctcacc ggcacgctca gcttcaatgc caccgcctcc
     5041 ttcacctcgg cgcgcatcac ccagggcgcc agcgggctga tcgccaaagc cggccaggaa
     5101 gtgctggccg tgcccaaccg cagcgccact ttcggccttg cctatcgcaa gccgatcgcc
     5161 gcgcggcgca gcctcttcgc ctcgttcgac tggacctaca ccggcaagag ctatggcagc
     5221 tacaaggcca ccgattccga ctatgcccgc cccgcctact gggtgggcga tctggatatc
     5281 ggcaccgaat acaacggctt tacgctgcgc gccttcatca agaacatcgc caatgatcaa
     5341 accatcatcc agcgcccgca agtgctgttc atcagccagg ggctgaccgt gcagccgcgc
     5401 accttcggcg tttcgctctc gcgggatttc tgagccggcc cacaggagat cgggcgacgg
     5461 gaatcaggca gcgggaatca ggcagcgggg atcgggcggc gcggctcagg ctgcggcggc
     5521 catcccctgc ctgcggcggc gcgcccagtc gccgccgcag aaggccttgc ggcgcggcgg
     5581 ctccttctgg ctctccagcg cttccatgca ctggcccaga taggcgcgcg ccagcacccg
     5641 ccgacgggaa tcgagataat cgcggcaggc ccgcaaggcc gcttccagct ggcgatcgtc
     5701 atcgatccgg tctgtcatgt ttctctcttt cgtttttgtc tttgggggcg gatccccgcc
     5761 gggcggtgat cgggataccc acaggtttac ggcaaagccc cgatggatgg gcgtaaacaa
     5821 ttgtaagtca tgttttttcc tgttgttaac cactgtgtca gcgggtaatc tgcggtttac
     5881 gtgcaggaaa accgcgcaga gtgtgaacaa ttgtaaagca ccaaggggca tagtcgtccc
     5941 ttgcgcattt tctgctaagg cgcttgtcag actggccatt ttcccttcta gtcgatagga
     6001 ggccccgcgg ttttccgcag ggcctaggca gatcggcagg ccgaacgggt gaatgcacgc
     6061 aggacgcttg gcttcaatga aaatcgcgat cctcgacgac gatccggccc acctcgactt
     6121 tgccacggat gtgctttgcc aggccggcca cagctgcgcg cccttctcgc aggggcgcca
     6181 gctgatccgc agcctgcatg acgaacactt cgatctcttc gtgctcgact ggaacctgcc
     6241 cgacatgtcg ggcctgtcgg tgctgcaatg gatccgcaac cagatcggca gcgcgccgcc
     6301 ggtgctgctg ctgaccagcc gcgtggccga tgaggacatg gtcgaggcgc tccacgccgg
     6361 tgccgatgat tatgtctcca agccctgcaa ggcgcaggtg ctgcaggccc gcgtgctggc
     6421 gctgggccgg cgcatccagc cggccggcgg cgagcgggcg gtggaaggct atggcgatta
     6481 tgaattcgac ctggccgccc gccgcgtgcg ccacaagggc gagcagatcg acatcacccc
     6541 caaggaattc cagctggcgc tgctgctgtt ccgcaatgtc aacatcgccg tctcacgcgc
     6601 ctatatgctg gaagcggtgt ggggcatggc gcccaccgcc gaaacccgca cggccgatat
     6661 ccacatgtcg aagctgcgcc gcaagatggc gctggggccg gccaacggct atgtcctgtc
     6721 gccgatctat ggctatggct atcggctgga ggaaatcagc gaggcatgaa ggggttggcg
     6781 cgtttttggg cggcgggcgc cctggtggcc ctggcgcccg gcatggccca tgccggcgag
     6841 ccggccgtga cccgcgccga aggcccgcgc ccggaaatcc gctacaccgt catccatggc
     6901 gacacgctgt ggcattttgc cgccacctgg ctggtggggg tgagcgcggc cgacgaactg
     6961 cagcggctga accacatccc cgagccgcgc cacatgccca tcgggctggt gctgcgcatc
     7021 ccggtcgatc tgctgcgcga ggaagcctcg gtggcggtgg tggaagcgtt ttcgggcccg
     7081 gtggccatcc tgcgcggcgg ccatagcgag ccgccccgca tcggcatgca gatcggcgag
     7141 ggcaccggcc ttgccaccgg cgccaatgcc ttcatcaccc tgcgcctggc cgatcgcacg
     7201 gcggtttcgg tgccctcgca atccagcgtc agcctggccc ggctgcggcg gcaattgctc
     7261 accggcacgc tcacccgcca ggtcgacgtg ggggccgggc gcctgcatgc caccgtcacg
     7321 cccatgcagg atccggccag cagcttccgg gtgaaaacgc cgctcaccgt ctcggccgtg
     7381 cgcggcaccg atttccgcat cgaattcgac gacaaggggc tggcggccac taccgtcagc
     7441 accggcaagg tggcggtggg cagcggccct gcgccgcgcc ccgaggccag cgcgctggtg
     7501 ccggccggct ttggccaggc caccaccccc agcggatcga gcgggctggt gaagttgctg
     7561 cccgccccgg aactcgccgc cgccgacaag ccgcaaaccg gcggcacgct ggcctttgcc
     7621 gtggcgccgg tggcaggcgc tgtgcaatat cgcgcccagc tggcgcgcga tgccggcatg
     7681 ctcgatgtgg tggaggaaag ccgcgctgcc gcccccctgc tgcgcctgcc gccagtggcc
     7741 gatggccatt acacgctgcg cctctcggcg gtggatgcgc aagggctgga aggcttcacc
     7801 cgcacctatc cggtggagcg gcagttgcgc cccgtcaccc tccatatgga gcagctgggc
     7861 cggggccagc gcctggcctt ccgctttggc tggacgggcg gcgtggccgg cgccagcacc
     7921 tggcatttcc agctcttccg cagccagtcg ggcgagctgc cgctgattga ccgggccgcc
     7981 atcgccgatg ccgccaccga agtgccggca ctgccgccgg gcgattatgc ctggcgcgtg
     8041 ggcgaggaaa acggcaccga cgccgactgg tcgccgccgc agcgcttcca catcgtcgcc
     8101 ggccgttgag cttgcccggg gcggataagg ccgccgcgcg ccagctggcg cggcaatggt
     8161 ggctggcgct ggcgggcacc gcggcgctgg tgctgctggc gctggccggc cacctcacca
     8221 cccggctcga caatgccttc tacgacgcgc tgatcggccg cgcccgccag ccggccgatg
     8281 ccggcgtgct gctggtggcg atagacgaac gcagcctgca gcagctgggc ccctggccct
     8341 ggccgcgatc gctgcatgcc cgcctgctgc ggcaactggc ggcgggcggg gcgcaggcgg
     8401 tggcgctgga tatcctgttt gtcgagccga cccggcctga agaggatgcc gccctggccc
     8461 aggccatgcg cgccgggccg cccgccttcc tgccggtgga atttgaagtg cccgggcgcg
     8521 atggcgcccc tttcaccctg gcgcggccga tcccgctgct ggcagaagcg gccgccggcc
     8581 ttggccatgt gaacctggcc cccgatgccg atggcaccac ccggcggctg tatctggcct
     8641 atcagggcca gggccacagc tggccgcagc tggccagcat gctggccggg gcgcgcggcg
     8701 gcacggcggc ggcaggtgcg gatctccatg gcacagcgcc ggtgctgctg cgctatgccg
     8761 ggccgcgcgg cagttttccc accgtttcag ccgccagcct gctggccggc gaagtgccac
     8821 cggcgatgat cgccggcaag ctggtgatca tcggcgccac cgccgccggg ctgggcgaca
     8881 gctatgccac gcccgccagc gccgatggct cgctgatgcc gggcatcgag atccaggcca
     8941 atctgcttgc caccctgcgc gctggccgag gcattgcgcc ggccggctgg gaggcggggc
     9001 tggctttcgc gctggcgccg ctgctgctgc tgatgctggc gctgcgcctg ctgccgccgc
     9061 gctggatgct ggtggcagcg ggcctgctgg tggccggcgt ggtggcgctg tcggccgggc
     9121 tgctgctggc gggcgatctg tggctggcgc cggcgccggg gctgctgggc atggcgctgg
     9181 cctatccctt gtggacatgg cgacggctga tggcggtcag ccattatctc tcggccgagc
     9241 tggagaagct ggatgccgag agcgacccgc tggagcgccc ccgcccgccg ctgcccgagg
     9301 gcgatgtggt ggcgcgccag atgggcctgc tgcgctcggc catcgaccgc gaacgcgatc
     9361 tgcgccactt cctcgtcgaa cgcatcgccc agatgcccga tgccgtgctg gtgtgcgatc
     9421 tggccggcgc ggtggtgctg gccaatcagg gcgcgtgcga tctggcccag gcgctgggcg
     9481 cgcaatgggc ggggccgggc gcccatgccg ccccgctgct ggcctgtttc acccgcaccg
     9541 gcgaggcgcc cgggccgctc gacctgccgc tggcagggct ggccgaaggc tgggaagggg
     9601 cggtggaggc ggccggcggg cgcagctttg ccctgcgcgc agagccccag cgcaatgccg
     9661 agaacgcgct ggtcggccag gtgctgcgca tcgtcgagac cagcgagcaa gtggccatgc
     9721 agcgccagcg cgaggatgtg ctgcaactgc tcagccacga catgcgcgcc ccccaggccg
     9781 ccatcatcgc cctgctggaa cagcaaggcg ccgccctgcc cgaaggcctt gccgcacaga
     9841 tcgccggcca tgccgggcgc accctcgcca tggccgacaa tttcgtccac ctctcgcgcg
     9901 ccgagctgat ggtgttcgag cccgagccgg tggatctgtg cgccatcgcc caggatgccg
     9961 ccgatgccct gtggccgcgc gcgcgccagc gcggcgtgga agtggtcacc accctgcccg
    10021 acgacgaact gtgggtggcc ggagaggcgt cgctgctggc ccgcgcgctg atcaacctgc
    10081 tcgacaatgc ggtgaaattc gcccccgccg gcgccactgt caacgtcacc gtggcagccg
    10141 cgggcggcat ggcctgcgct catgtggcca accccggccc gcccattccg ccagcccaaa
    10201 tggcccgcct gttcgagcgc ttcacccgcg ccggcagcgg ccctgcccgc aaggccgatg
    10261 gggtggggct ggggctggcc tttgtccacc gcgtggccac ccggctgggc ggcaccatcg
    10321 cctgcaccag cgatgcagca cagggcacct gcttcaccct caccctgccc cgcctgcccg
    10381 actaggcgaa accgggcgtt tacgccgcgt tcgccgcgat ataggcgtcg atctcccccg
    10441 ccagcaccgg cagcggcacc gcgcctggca gcaggccagc atcgtggaac gcgcggatat
    10501 cgaaacgcgg acccagcgcg cgccgggcct tctcgcgcag gcgcagccat tccagcttgc
    10561 ccagcatata gctgcacgcc tgccccggcc acacgcaata gcgatccacc tcggtggcag
    10621 cggcggcctg cgggctgccc agaatggcgc gataataggc aatggcacgc gcccggctcc
    10681 acccttgcgc atgcaggccg ctatccacca ccagccgcac cgcgcggaac agggcatcgt
    10741 ggatctgccc cagatggccc agcggatcgc ccgcataaag gcccatctcc accgccagct
    10801 gctcggcata cagcgcccag ccctcgccat agccggagaa gaactgcatc ttgcggatca
    10861 gcggcagccc cgcctcctgc cccaggctca attgcaggtg gtggccggga atgccctcgt
    10921 gaaacgtcag cgtcggcaac gaccagcgcg gcacctcggc cgtatcgcgc aggttgatgt
    10981 aataggcacc ggggcgcgaa ccatccagcg cgcccggctg gtaatagccg cccggcgccc
    11041 ccgcctcgat ctcgcgcggc acgcggcgga tcgccagatg ggcacggggg cgggcgccaa
    11101 accaggccgg caacagcgct tccacccgcg ccaccagccc ggccagatcg gccagcaatt
    11161 gcgccttgcc cgcctcggta ttggggtaaa gctggtctgg ccgggcatag agcgcacgca
    11221 tccgcgcccc caccgaccct tgcgacaggc cctgccgccg caggatcgca tccgcctcgc
    11281 cgctcagccg cgccaccagt gccagcccct cgcggtggat ctcggccggc ggcatcgtgc
    11341 tggtggtgta atactgcagg gacagcgcat agagcgcccc gccctgcggc agatggccca
    11401 cgcccgccgc cgccccggcg cgcggcgcca ggctttccag caaagcgatc tggcgggcca
    11461 gcgccgggcg caccagccct gtgtaaacgg ccccggcgcg cgcttcccaa tcaccggcga
    11521 tggcctgcgc gcgggcgcgc cgggccagcg attgcaccag attgctggca tcgctgccct
    11581 gccctgccag ggcgcgcagc tgctgcagcg ccgttgccag aatgaaggcc ggcggcacca
    11641 cgcccagcgc cgcatcatgg cggacctgtt cgttttcctg atcgagcgcg gtggcaaagc
    11701 cggccagccg cgccagatag gcctcggcat ccgcggcgct ggcgatgctg tgctggctgt
    11761 cgagaaaatc ggggatctgc tgataggcgc cggtcaactg cgagacggca tagggcatgc
    11821 cggcataggg cagctggcga tagagcctgt cctgcaccgc cagctggaac accaccgacg
    11881 cgaggttggc cctgtcccgc ggggccagcg ccgcccgatc gatcgcctgc aaggcggcca
    11941 gctgcgcgcc attgcgggcc ttgtccgcct gccaggccgc cagcgagcgg tcatccagcc
    12001 gggccttcgc cccggcccgc gcgccgcgat caagccccag gctggtcacc gtttccggcg
    12061 agcggtcgag catctcgcca gcgatccgat caaacagcgc ggccagcctt gccccttcgg
    12121 ccccttgcgg ctccggcccg gccagcgcag caccgccaag gccggccatg gccagccctg
    12181 ccgctgtgcc cctgatcatg ctcctgcggt ccatcggcgg gctccttcgc atgcctgtta
    12241 cggcctgctg catgcctgcc gcaacggccc cgcgcaaggc gcccgccgcc ggctcagacc
    12301 agccgcgccg cgttatgccc cagttcggcg aaatagcgcg ccatggcatc ggcggccttg
    12361 tcggtcaggg tgatgaaggc gcggcggcgg tccgtctcgt cttccatgcg ctgcagcagg
    12421 cccgattccg tcatctggcc gatccagcgc agcgccgtcg tcggcggcac gcccgaggcg
    12481 atgcacaggc tagtgaccga cacgcgcgca tgttcggccc gcgcggcagt cagatccagc
    12541 agcatttccc aggccggatc ggcaaacaga tcaccatcga gatagcgcgc gcgcagctgc
    12601 cgctggcgga tgatgcgccg caccagccgc ggatcgggca gcggcgggcg cggcgcgcgg
    12661 gtgtatttgt cgccctcggc agccgcttct gcctctgcct cggtgctggc caggcgggcc
    12721 acggtgcctt cgcgcgtggc cgcggcggcc agcgcggccg ggccgccgcc gggcccgccc
    12781 agccggtcga tccgctcggc gatctgggtc acctgctcgg tcaggcgcag cagcaccagc
    12841 cggtcctcgt ccgacagttc gcgcaccttg cgctggggca tgcgcgccag cacctggccc
    12901 agcgcgatca cccgctccgc gcggctgggc gccaccagga tctgcgcggc cgactgatcg
    12961 aggcagccga acacatcgtc cagcccgtcc acgctggtgg agacgaccag ctcgccgccg
    13021 gcctgcgcca cgcgcatgtc gatccgcgcc agcgctgcca gcgccgcgcc atcgggcgcc
    13081 gggcaatcca ccagcaccac atcgcccagc cccgacgccg tgccatcatg cagcgcggcc
    13141 agcggcgcca cctcgcccag gcggaagccc gccccttcgg catcctcgca gatttcttcg
    13201 cgcagcaggg cgcggtcggc aaagacggaa acagtcaacc gcaaggcgct gccggcatgg
    13261 gcaggcgcgc catagctgaa atcggcaagc gtgtcggtga aagcagcaag ctgggccttg
    13321 ggcatgatgc gtactcccct gcgttcaata cgcgactata cgtagaacaa agcggaaata
    13381 cgtcaataca cttagagaac attacaggat caaatagatc tattccggag gaatcagatg
    13441 cttggcctgc ctatcggctg gccataaacc cgccccttcc cgcccatcca tacgaaaaag
    13501 ccccgcccgg cgcgaatcgc caggcggggc cttatctccg tttcggatgt aatgccggcc
    13561 cggcgcctca atcggcgaag ggatcgcgca ccagaatcgt gtcctcacgc tcggggctgg
    13621 tggaaaccag cgccaccggc gtctcgatca gttcctgcac gcgctggata tatttgatcg
    13681 cttgcgctgg cagatcggcc cagctgcggg cgcccgccgt ggtgccctgc cagccatcca
    13741 tttcctcata gatcggctcc accgcggcct gatcggcggc atgggcgggg tagtaatcca
    13801 ataccttgcc atgcaggcga tagccggtgc agatcttcac cttttcaaag ccatccagca
    13861 catcaagctt ggtcagcgcg atgccggtca cgccggaaat ggcgcagctc tggcgcgtca
    13921 gcaccgcatc gaaccagccg cagcggcgct tgcggccggt aacggtgccg aattcatggc
    13981 cgcgctcgcc caggcgctgg ccggtttcat cctccagctc ggtggggaac gggcccgagc
    14041 cgacgcgggt ggtgtaagcc ttcacaatgc ccagcacgaa gccggtggcg ctggggccaa
    14101 gcccgctgcc cgaagcggcc gtgccgctca ccgtgttcga cgaagtgacg aagggatagg
    14161 taccatgatc gacatcgagc agcacccctt gcgcgccctc gaacaggatg cgcgcgccgg
    14221 ccttcttcac cgtgttcagc cgcttccaca ccggctgggc aaattgcagc acgaagggcg
    14281 cgatggcgcg cagctcttcc agcagcgcgg cgcggtccac cggcggctgg ccgaaaccgg
    14341 cacgcagcgc gtcgtggtgg gcgcacagcc ggtccagctg gggatccagc gcatccagat
    14401 gggccagatc gcacacgcgg atggcgcggc ggcccacctt gtcttcatag gccgggccaa
    14461 tgccgcggcc ggtggtgccg atcttgccgg cgccggcggc cgtctcgcgc aggccatcca
    14521 gatcgcggtg catcggcagg atcagcgggc aattctcggc gatggcgaaa ttctcggtgg
    14581 tgatcaccac cccctggcct tccagcttct ccacctcggc cttcagcgcc caagggtcca
    14641 gcaccacgcc attgccgatg atcgacagcg tgcccgtgac gatgcccgag ggcagcagcg
    14701 acagcttgta gaccttctcg cccaccacca gcgtgtggcc ggcattgtgg ccgccctgga
    14761 agcgcaccac cgcatcggcg cggctggcca gccagtcgac gatcttgccc ttgccctcgt
    14821 cgccccactg ggcgccaatg acggttacgt ttgccatggt tgcaagcctc cacctgcccg
    14881 gtggatccag aagcgccctt cgacaggact cggcgcagcg gatggaatcg tttgggccgc
    14941 acagggcccg gttgcgcggc ggcgggtagc cgggccgcgc aggggtgtca agcgggccgg
    15001 ccgatcaggc gggggcgatc aggccagcac cgcctttgcg ccctgcagca catggctgca
    15061 gcccagcgcg gccgccgcat ccccctcgcc cagcgctgcc accgtgcgcc agcctgccgc
    15121 gcgcagggcc gccgctgctg cctcatcatg gcccagcggc aggaacagcg ccgcgcgcgc
    15181 cggctctgcg gcggccagcg catcgatcag cggatcggga tagagcgaga agccagtggc
    15241 cggctcaccc gccgtgccct cggcgccaag gatgcgatag gtgccgccgc gccccacaga
    15301 gccgcgcagc ccttcggcaa acagggtgaa gccaaaccag ctctgatatt caaagccatg
    15361 ccgctcggtc gggtccagcg tcagccgggc cttgccggcc acccgcgcgg cgatctggcg
    15421 cagggcggca atccgcccct ccagcgcgcc gcccgccggc agctggccca accgctccag
    15481 cgccggctcg aaagcgccgg tggcatagag cagcggcaga taggcctgac cgcccgcttc
    15541 caccagcgcg ctggcgttct tggcatccag cgcggcgcgc accgcatcga tggcgccggc
    15601 cggcagcggc agcggccctg cggccagcgt gtccaccaga tcgggcaggg tgaaatccac
    15661 cgaaaggcca gtggcgcccg ccgccaccag cgcttccacc gccacggcca cgatctcggc
    15721 ggcggcggcc acgctgtcgg cgccgatcag ctccacgccc agctgcaggc gttcacgcgt
    15781 cgggtccagc ccgtcgccct tgatcgtcag cacctgcccg ccataggcca gacgcagcgg
    15841 ccgcgccgcc cccgccagcc gggtgacggc aatgcgcccc acctgcgggg tgatgtcgga
    15901 acgcagcgcc agcatgccca ggctcgccgg atcgacaaag cggaacatgc ggcgcggcga
    15961 aatgcccgcc atgcggctgg ccagcggcgc ctcgaactcg atcgaggggg gctgcacgcg
    16021 gtcgtagcca tgcgcgtcga acacatccat cagcgcacgg gtgatgcggg tggccgcggc
    16081 cgtggcctgc ggcagacggt cctcaagccc ctcgggcagc agatcgcggg tggtgtcggt
    16141 catggcgcgg gctttagcgc cggtttggcc aaaggggaag ccggatgcgc agagcctgcc
    16201 gccaaacgga aaaaggcgag gccaccgggc cccgcctttt ccttgttcct ggcctggcgg
    16261 ccgcgatcag aaggccagcg ccttcacccg cttcacgccc ggcagcttgc cggcggcatc
    16321 cagcacatcg gcgcccaccg gattgtcaac cgagagcagc aggatcgcct cgccaccggc
    16381 ctcgcggcgg cccaggttga aggtgccgat gttgatggca ttttcaccca gcagcgtgcc
    16441 gatgcggccg atgaagcccg gcgcgtcctc gttgacgata tacatcatgt cgccagccag
    16501 atcggcttcg accttgatgc cgaacagttc gaccaggcgc ggctcgcggt tgttgaacag
    16561 cgtgccggcc accgaacgct cgcccgcctc ggtcttcacc gaaacgcgga tcagcgtgtg
    16621 ataatcgcct tccttctcgg tcttcacttc gcgcacttcc agcccacgct ccttggcgag
    16681 gaacggcgcg ttgaccatgt tcaccgtggc cgactgcacc ttcatgaagc cggccagcac
    16741 cgccgccacg atcggcttgg cgttgagctc tgccgcggcg ccctcggtgt ggatcgagat
    16801 gcgcgccacc gccgaagggg tgagctggcc caccaggctg cccagctttt cagccagcgc
    16861 catgtagggc ttgaggcgcg gggcttcctc agcgctcagc gagggcatgt tgagcgcgtt
    16921 ggtaacgccg ccggtggtga gatattcgcc cagctgctcg gccacctgca gggccacgtt
    16981 gacctgcgcc tcggtggtgc tggcgcccaa gtgcggcgtg cagatgaagt tcttggtgcc
    17041 gaacagcggc gagtccttgg caggctcggt ctggaacacg tcgagcgcgg cgccggcgat
    17101 atggcccgaa tcgaggcctt ccttcagcgc ggcctcgtcg atcaggccac cacgggcgca
    17161 gttgacgatg cgcacaccct tcttggtctt ggccagattt tcagccgaca ggatgttctt
    17221 ggtgtcagcg gtcagcggcg tgtgcagggt gatgaaatcg gcgcgggcca gcaggctgtc
    17281 cagctccacc ttctccacgc ccagttccac ggcgcgctcg ggcgtcagga acgggtcgaa
    17341 ggcgacgacc ttcatgcgca ggccaagggc ccggctggcc acgatgctgc cgatattgcc
    17401 ggcgccgatc aggcccagca ccttgccggt cacttccacg cccatgaagc cgttcttcgg
    17461 ccatttgccc tgctgggtct gctcattggc ctcggggatc tggcgggcga gggcgaagat
    17521 catcgcgatg gcgtgctcgg ccgtggtgat cgagttgccg aacggcgtgt tcatcacgac
    17581 aacgcccttg gcgctggcat aggggatgtc cacgttatca acgccgatgc cggcgcggcc
    17641 gatcaccttc aagttggtgg cagcgtcgag gatctccttg gtcaccttgg tcgagctgcg
    17701 gatagccagg ccatcatagt cgccaatgcg ggcgatcagc tgctcgggcg tctcgccggt
    17761 gatcacgtcc acgtcgcagc ccatctcggt gaagatgcgg gcggcattgg ggtccatctt
    17821 gtcggaaata aggacgcggg gcttgcgggt ctcggtcatg ggaagctctt cctcatgcag
    17881 aattttcaaa gggggaaagc atgggggcgc gcagcaacag gcgcccccat gggcaatcga
    17941 taccggatca accggccttg acggtctcgt aggcgtagtc gagccagggg cccagcgcct
    18001 cgatgtcttc cacgttcacc gtggcgccgc accagatgcg caggccagcc ggcgcatcgc
    18061 gatagccggc gatgtcgtag gcggcatcgg ccttttcgag cacagcggca aacttcttga
    18121 tgaagtcggc atcggcgccc tcaacgctga ggcacaccga ggtcttcgag cggatcgcct
    18181 cgtcggcagc cagatgcgag agccagctgc gctcgtcaac gatcttgccc agcgcggccg
    18241 cattggcatc cgaacgcgcc ttcagccctt ccaggccgcc caggcccctg gcccattcca
    18301 gcgcgaagat cgcatcttcc accgccagca tgctgggggt gttgatcgtc tcgcccttga
    18361 acacgccctc ggccagcttg cccttgctca tcaggcggaa caccttgggc agcggccagg
    18421 ccggcgtata ggtctccagc cgctccaccg cgcgggggcc gaggatcagc acgccatggc
    18481 cgccctcgcc gcccagcacc ttctgccagg agaaggtggc cacgtcgatc ttcgaccagt
    18541 cgatgtcgta ggcaaacacc gccgaggtgg catcggcgaa gctgaggcct tcacgatccg
    18601 ccgggatgaa atcggcattg ggcacgcgca cgccgctggt ggtgccgttc caggtgaaca
    18661 gcacgtcgtt cgaccagtcg atcgcggaga ggtcagcgat ctggccataa tcagcgcgca
    18721 gaatggtggg gtccagcttg agctgcttgg caacatccgt cacccagccc tcgccaaagc
    18781 tttcccaggc cagcgtggtg acggggcgag cgcccagcat cgtccacatc gccatttcga
    18841 aagcgcccgt gtccgaaccg ggcacgatgc cgatgcggtg cgtttcaggg agctgaagga
    18901 tttcgcgcat cagatcgatg caataggcaa ggcgcatctt gccgatcttg gcgcggtgcg
    18961 agcggccgag cgattcggtg gccagctggg caagatcgta gacaggcggc ttggcgcagg
    19021 gccccgaaga aaagaagggg cgagccggct tggtggccgg agcattcaca gtcatgtaga
    19081 ctctcctcac agagagcacg cgcggcgttg ggaccgcgtg gcccgctggc cgctctagag
    19141 gcccgcccgg cagagtcaag cgcccgatgg ggcgggcgat cattttttct aatgcccgcg
    19201 cggcaggccg ccggcttttc cacaagcagc ttggccgcca tggattcccg ccagcgccgc
    19261 tttggcgcac aagggctgca tgacgcgccg gatgatcccc ctcattctgc tgccccttgg
    19321 ccttctcgcc ggctgcctgc cgcaggatca gggccaccag ccgccgcgcc cgcgccaaca
    19381 ggcccgcgcc tatgccccct cgcccgccgg cgcccagtgc atggccgatc tgcaccagcg
    19441 ccaggccggc tttgcccccc tgcccgatgc ctattacggc ggcggctgcg ccaccatcaa
    19501 caccgtgcgc ctgctcagcc tgcacggcga taccgatgcg ctggcggtca gcaatctggg
    19561 ccctgtcacc tgcccgctgg ccgatgcctt tgccggctgg gcgcgctatg gggtggacag
    19621 ggccgcgcgg ctggcgctgg gcagcccgct cgcccggatc gagacgatgg gcagctacaa
    19681 ttgccgcaac atcgccggat cagaccatcg aagcgcccat gccaccgccg atgccatcga
    19741 tgtgtctggc ttcgtgctgg ccgatgggcg gcatatatcg gtgctgagcc aatggagcgc
    19801 gggcacggcc gaggagcgct atttcctgcg cctggtgcac accagcgcct gcaagcgctt
    19861 cggcacagtg ctggggccag actataaccc ggcccaccgc aaccatttcc acctggaact
    19921 ctcggccgcg ggattttgtc gttagtttgg cggcaccagc ccgctccccc acccggccac
    19981 cccacgatag tgccctaggg gtggccgggt gggggag
//
LOCUS       DASRYE010000111        19137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_6378,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000111 DASRYE010000000
VERSION     DASRYE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19137
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6378"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFF98_06125"
     CDS             <1..244
                     /locus_tag="VFF98_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZU63240.1"
                     /translation="EAAQFLKDEAKRGRKKPMVGFIAGRTAPPGRRMGHAGAIVSGGQ
                     GGAEDKIAAMEEAGIRVSPSPSLLGETLAELLKEIA"
     gene            423..3260
                     /locus_tag="VFF98_06130"
     CDS             423..3260
                     /locus_tag="VFF98_06130"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZU63241.1"
                     /translation="MGDESHDFLTDADLNGPQPGPSWQRGNWPQVGGAAENDLTQALD
                     PTILKPAIKEAAKKAGAPLSEAAVEEAAADSIRAQMLIRTYRVRGHLAADLDPLGLSK
                     RDIPADLTPEYHGFVGAALDRKVFLGGTLGLQWATVKEIVAILRANYCGKVGLEYMHI
                     TDLEERKFLQDRMEGADKHIDFTPNGKKAILGAVIRGEQYEKFLGKKYVGTKRFGLDG
                     GESMIPALEAVIKYGGQLGVKEIVYGMAHRGRLNVLANVMAKPYKVIFHEFSGGSANP
                     DDVGGSGDVKYHLGTSTDRDFDGIKVHMSLMPNPSHLETVDPVVLGKVRAYQVFRGDV
                     GEGKHKQVLPVLIHGDAAFAGQGIVWECFGFSGVHGYNTGGCIHFVINNQIGFTTSPQ
                     FSRGSPYPSDVAKGVQAPIIHVNGDDPEAVTFACKFAIDYRQTFGRDIVIDMWCYRRF
                     GHNEGDEPSFTQPLMYAKIRQHKPVSEIYAERLQRDGVIDGGFVGEQVAAFTAHLEEH
                     FDAAKTYQANEADWFGGRWSGFNRPMDPETARRNVPTGIEGKLFDSLGRTLTTVPDHL
                     TVHKTLGRVIDAKREMFTSGEGFDWATGEALAFGALLSEGYAVRLSGQDSGRGTFSHR
                     HAVWVDQNDEHKFIPLSTVPHGRFEVYDSPLSEYGVLGFEYGYASADPKTLVLWEAQF
                     GDFANGAQIMIDQYVASSESKWLRANGLVMLLPHGYEGQGPEHSSARLERYLQLCAQD
                     NMQVCNITTPANYFHVLRRQMHRPFRKPLIIMSPKSLLRHPMAKSKAADFIGDGHFMR
                     ILSDPTPAEDAKTKRVVLCSGKVAYDLIEARDAASLADTQIIRIEQIYPFPGEPLAKR
                     LARMGALEEVVWCQEEPKNNGSWFFVEPFIEDVLKAAKAPVTRARYAGRAASASPATG
                     LAKRHAAEQAALVADALGLSVRSEIRRKQKA"
     gene            3305..4519
                     /gene="odhB"
                     /locus_tag="VFF98_06135"
     CDS             3305..4519
                     /gene="odhB"
                     /locus_tag="VFF98_06135"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HZU63242.1"
                     /translation="MSTEVKVPTLGESVSEATIGQWLKQPGEAVALDEPIASLETDKV
                     AIEVNAPVAGVLTAQLAEVGATVTVSAIIATIEAGAAAAAPAAKPAAAPAAPAAVAPV
                     PVAAAAEATDPAVLSPAVRRAVLEYGIDPSTVKGTGKDGRITKEDVIAAAANKAASPA
                     AAASVPAAPANERREERVKMTRLRQTIAKRLKSAQDTAALLTTFNDVDMSAVIEARAR
                     YKDTFEKKHGVRLGLMSFFAKASCLALKDIPAVNAQVDGDEIVYHDYVDLSVAVSAPN
                     GLVVPVVRNVDKLSFAEIEKAIAGYGKKAKDGALTMDDMKGGTFTISNGGVFGGLMST
                     PIINPPQSAVLGLHRIEDRPVVRNGEIVIRPMMYIALSYDHRIIDGREAVTALKTIKE
                     AIEDPTRLLIDL"
     gene            4542..5942
                     /gene="lpdA"
                     /locus_tag="VFF98_06140"
     CDS             4542..5942
                     /gene="lpdA"
                     /locus_tag="VFF98_06140"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZU63243.1"
                     /translation="MAEYDYDVLVLGAGPGGYVAAIRAAQLGLKVACAEGRATLGGTC
                     LNVGCIPSKALLHGSELFEEAAHGTLAKFGVKTGPVELDLATLQGEKASAVKELTGGI
                     EYLFKKNKVDWLKGYGTFKSAHEVDVAGKVVSAKHVIIATGSSVAPLPGVAVDNDAGV
                     VVDSTGALALDRVPQHLVVIGGGVIGLELGSVWRRLGAQVTVVEFMDQLLPGMDGEVR
                     KEAAKIFKKQGFELKLSTKVTGAVVEGGKATLTLEPAAGGEATTLEADAVLVAIGRRP
                     NTEGLGLENIGLVLNKRGQIETDHDFRTAVDGVWAIGDVIPGPMLAHKAEDEGIAAAE
                     NIAGLTGIVNHDVIPGVVYTSPEIAGVGLTEEAAKERGAIKVGKFPMLANSRAKTNRE
                     PDGFVKVIADAATDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSE
                     AVKEAAMAVLGKPIHI"
     gene            complement(6013..7533)
                     /locus_tag="VFF98_06145"
     CDS             complement(6013..7533)
                     /locus_tag="VFF98_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63244.1"
                     /translation="MWLLLHRQKLIAWATGLALLATALAALCARGAWYDEFYTFYVTR
                     PGLSFAQGWPVWLRDNHPPLFYALAWASNGLGQLHGGQLHGGMLGEGGVGPRRLVNLA
                     FLLGGMGALAWLAVRRAPLRLVLLAYGLGLASNYQTINRVAELRSYALVLVSGAVALV
                     LLADVARPGARVSRSAWAALVAALAVALSVHLVASVVMGALGLAFGLRLLLARDWPAA
                     RALALAGVLAVLPFAASMALQWRTMAANTASFWIPPGLSAARWAVENEVLANLAGNRL
                     LSLAGLAGLALVALRDLRARRLSGEGSLVLTLAGGVALAVALLVALHLHRPFIINRYL
                     VCLHPPLTLALAVGYAALARALRPAAAALLDGALFLATLWALHAHYVATIAQPSWEGT
                     ARAIAAATRACPATLVYANTSWNAPTLDLPPPDNRAVVPFAYGWVAAHHGFALTPIAA
                     RRLSPTCPTLFWTEHVAGQYPSAEAVAQRLREGGFAVPAGQLRRYGEGWVFSVNAR"
     gene            complement(7586..8227)
                     /locus_tag="VFF98_06150"
     CDS             complement(7586..8227)
                     /locus_tag="VFF98_06150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU63245.1"
                     /translation="MAGPDSGALLPPGAEHRGAQRFTLLVRTAKLVIDGREYLCVLRD
                     ASNTGVKVRLFHPLPAQGQIALELGSGERYPMELVWQTSDHAGFRFLGDIDVHRLIED
                     KRDQYPKRQIRMRLGCIARLVAHGHDGPVLLRDISQQGACIETGERLMLRQPVRLEAE
                     GFPPVHAKVCWRHQPRYGLVFEKGFSLEELALHLARLHAAAPATGAVIDRYAG"
     gene            complement(8483..11152)
                     /gene="secA"
                     /locus_tag="VFF98_06155"
     CDS             complement(8483..11152)
                     /gene="secA"
                     /locus_tag="VFF98_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZU63246.1"
                     /translation="MFGKLAKSLFGSSNDRYVKSLDKLVQKIAAFEPAMQALSDAELA
                     GQTVKFRAALAEGATLDSLLPEAFATVREAAVRAMGMRHFDVQMIGGIVLHRGEIAEM
                     KTGEGKTLVGTLAVYLNALEGKGVHVVTVNDYLARRDAETMGVLYNFLGLTVGVIVPN
                     LDEVQRREAYNSDITYATNNELGFDYLRDNMKHTREQMVHRPFNFAIVDEVDSILIDE
                     ARTPLIISGPTDDKSELYVAVDAIVRHLVAEDYEADEKAKNITLTEEGVEKAERLLEN
                     AGLLVGSNLYDIENTQVVHHLDQALKANVMFKRDIDYIVKDGKVVIIDEFTGRMMDGR
                     RWSNGLHQAVEAKEGVKIEPENQTMASITFQNYFRMYPKLSGMTGTAATEAPEFFDIY
                     KMNVVSIPTNVPVQRIDEEDEFYKNTHDKFAAIAKLIREKNEIGQPVLVGTVSIEKSE
                     LLSEFLTKEEVKHNVLNARFHEMEAHIVAQAGRLGAVTIATNMAGRGTDIKLGGNLEF
                     RINDELKDAPEGAERDAAIERIKAEIEAEKQQVLAAGGLAVIGTERHESRRIDNQLRG
                     RSGRQGDPGLSKFYLCLEDDLLRIFGPDTLFSRMMNANLADGEAIGSRWLSKAIETAQ
                     KKVEARNYDVRKQVVEYDDVMNDQRKVIYEQRADIMDAETLDDVVAEMRHDTVNTLVA
                     TACPHGSYPEQWDTEGLKARALELLALEIPLDDWLAEDGIEPDTLEQHISTFADERMA
                     AKQAENAEIWRQVEKSVVLERLDHYWKEHLATLDALRQVVFLRAYAQKTPINEYKQEA
                     FGLFERMLDTIREDVTRILSHAELQFAPPEPALPELPDFLTTHTDPFTGENEAGPPAI
                     PGVDPYADMGLNRNALCPCGSGQKYKHCHGAVA"
     gene            complement(11289..11555)
                     /locus_tag="VFF98_06160"
     CDS             complement(11289..11555)
                     /locus_tag="VFF98_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63247.1"
                     /translation="MLALLAMLTGLLLQVPAGMAMPVGTAPAVAAASLGAARAAVVAA
                     RVQAVAAVRAVAGEQPAWAAARPLPAVAAPAIPAVRLRADRTRE"
     gene            11657..12883
                     /gene="argJ"
                     /locus_tag="VFF98_06165"
     CDS             11657..12883
                     /gene="argJ"
                     /locus_tag="VFF98_06165"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZU63248.1"
                     /translation="MSHAISPLASPFPALAPIAGATARIARAGYKDWGRCDLTFVELD
                     EGTSVAGVFTRNVCCSSEVELGRLNVAQGQARALIVNAGNSNAFTGYRGREAVEQIMD
                     QVAAHLACPREQVFVSSTGVIGVPLPKDKAREGVAAALAAAPCNWEEAANTIGTTDTF
                     AKGASATAIVDGHTVTFSAIIKGSGMIAPDMATMLGYIFTDAAVAPAFLQALLSRAAD
                     ATFNCITVDSDTSTSDTVLAFATGKAGNAPITGADSAGADAFAAALHDVCRQLAQLVV
                     KDGEGAQKFIAITVSGAVSDESARKVGLAVANSPLVKTAIAGEDANWGRVVAAIGKAG
                     QPADRDRLSIGFGGVWAARDGQPLADYDEAPVAAHLKTREITIEIDLGLGEGRATVWT
                     CDLTHGYISINADYRS"
     gene            12888..13514
                     /locus_tag="VFF98_06170"
     CDS             12888..13514
                     /locus_tag="VFF98_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU63249.1"
                     /translation="MLTIWGRLNSHNVKKVVWAAGEAGIAWQRRDMGGSFGYTPAYLA
                     HNPNHLVPMIEEADGFTLWESNAILRYLASAHAPHLWAGDARQKARADKWLDWQFAFA
                     DAQRDGFLQLVRVAEAQRDAAKIAATAAATGRLMAMAEAELARQPFLSGAEFGIADIA
                     MGTYAHTWFTLGFDRAEGGEKAEVPHVRAWHAALIARPAFAAIAIPLS"
     gene            13525..14325
                     /locus_tag="VFF98_06175"
     CDS             13525..14325
                     /locus_tag="VFF98_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZU63250.1"
                     /translation="MTDSLTEAVYHVMADAARRAITPRYQHLGAGEIIEKAADDLVTV
                     ADRESEAILAEGLARILPEAAIVGEEAAFADPAILGRLGEDLCWIIDPLDGTHNFAHG
                     KPPFGILIALSQSGETIGGWIYDVLSGRFCHARRGEGAFIDGEPIRARSSGEEPPIGA
                     ISVLFADAERRAAITAHVAPHYRLVDIPRCAAEQYPRLATGVNDVSIFERTLAWDHAA
                     GVLFLNEAGGKAARFDGSAYRVDDARKGLIGAASPALWEALATRMAEL"
     gene            complement(14363..14683)
                     /gene="trxA"
                     /locus_tag="VFF98_06180"
     CDS             complement(14363..14683)
                     /gene="trxA"
                     /locus_tag="VFF98_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZU63251.1"
                     /translation="MATITVNDASFATDVLGADKPVLVDFWADWCGPCKMIAPALEEI
                     AEELAGQVTIAKVDIMENTSTASQFGVQSIPYLVLFKNGQPVATKLGAAPKSQLKGWI
                     ESVI"
     gene            complement(14737..18330)
                     /gene="addA"
                     /locus_tag="VFF98_06185"
     CDS             complement(14737..18330)
                     /gene="addA"
                     /locus_tag="VFF98_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HZU63252.1"
                     /translation="MSGTQLRPHPLAGNQMRAVDPARTVWLSASAGTGKTQVLSARVL
                     RLLLQPEVRPEQILCLTFTKAGAAEMAERIHSRLAGWVRADPVRLAMELEAIGADIGP
                     ETQARARTLFAAVLDCPGGGLRIDTIHAFSQWLLAAFPREAGLAGGARPLEDQQKRLL
                     ARQVLADLLRDAESGGDAAMLAAVEALALRLGLDGVEGWLMACASARSLWFGPGCWVP
                     PLAPHVARVLGLPADADAAGLAALCADEAFDTRSLRELLAACKGWTDKKGNLTANAEK
                     KANAIGDWLAAPPEARLAGLDLLYKALFTEKDEPSYLASLVKIAPDYGEYCYRVGERI
                     NAIREQRALLALAGWLAPALEVGRRYALAWDAAKAREGVIDFDDQIQLAADLLSRAEM
                     ADWIRYKLDRRIDHVLVDEAQDTNAAQWRIIRAMVGEFFSGEGARTSAWGGPGGKPPP
                     RTLFVVGDYKQAIFGFQGTSPENFARAGAWVKGEMAAATANARQQRGGEEAEGLLELG
                     LERSFRTARPVLDFVDLAMARMGAAQFGLDRPAEPHQGQPRPGYVALWAPVGGRPDEE
                     EEDEPAGEDEGPAVAQNWLSRTDRQMAERLAAQVKAWLADGFALVKGEKEGEARAATP
                     GDIMVLVRKRRDLAGLIVARLHAAGVPVAGVDRLRLAAPLAVRDLVAALRFAAQPLDD
                     LNLASLLVSPLVGWSQAQLLAHGHRPRGTRLWDHIRRSPHAEAGAVREQLAPLLARAD
                     FDTVPALLQWLLVGPWAGRARLLTRLGAEAADPIEELVNAALAHAASATSSLAGFLDW
                     FDGAEGELKREAGNSGDRVRVMTVHGSKGLQAPIVILADAAGDPRLGREGAARVDDPA
                     ALLGGGEPRQMPLPRLRKGERLGPIKAADAAALKAESEEHWRLLYVAMTRAEEALFIG
                     GALARRETAPADDSWFARLADVAEAGGGEGRLDDPLWGWRLEYGARATAPIRRAGAAA
                     SLPLGPALPGWLLRAPAEEPVPPRPLSPSALGEDHSANPPTGMANSADRARAARRGTL
                     MHKLLERLGAVPADARAPLAEAWLARQAGDVEASLRAEIAATALAVIGDARWADLFSP
                     AALAEVPVAAVVAGQVIAGTIDRLLVEEGRIRLVDFKSARRPPTRLDQVPVAILRQMA
                     AYAAALAEAFPGRPVEAALLYTQSPRLIEIPVDVLERHKAGLGLAQESLAL"
     gene            complement(18327..>19137)
                     /locus_tag="VFF98_06190"
     CDS             complement(18327..>19137)
                     /locus_tag="VFF98_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZU63253.1"
                     /translation="TALDRLRADPYQFYAQAILGLARLDPLDSPPTPAWRGTAVHGVL
                     DAWHRAGAPVGGLAQEGAAMLDRLNAHPLTRSLWRPRLLAALEWIDAELLRLKGEGRQ
                     VLATEIRGEIRVDGIRIHGRADRIDRLPEGPLAIVDYKTGKPPSGKQVAEGFALQLGL
                     IGLIAEGGGFKGIGGEAEAFEYWSLSARKGGDGFGWREEPIMEGRKRSGIPRADFLPE
                     TERFLREAIARWLLGAEPFTARLNPDIGGYNDYDQLMRLDEWIGTLEGGAA"
BASE COUNT         3182 a         6726 c         6264 g         2965 t
ORIGIN      
        1 agaggccgcg cagttcctga aggatgaagc caagcgtggc cgcaagaagc cgatggtggg
       61 cttcattgcc gggcgcacgg cgcctccggg ccgccgcatg ggccatgccg gcgcgatcgt
      121 ctcgggcggc cagggcggcg ctgaagacaa gatcgcggcg atggaagaag cgggcatccg
      181 cgtctccccc tcgcccagcc tgctcggcga aaccctcgcc gaactgctga aagaaatcgc
      241 ctgatactgt gatacaccgg gcagggcggc gccttggcgc atccctgccc ggatctgact
      301 gacccggcct tggcgcccct cctcccccct cagcgccaga ccttaaaacc tgccggacgg
      361 ctctattgcg cattgcgtgc cccaggcaca gccggacgca gggcaacaaa aggtgtttcc
      421 ccatgggtga cgaaagccac gattttctga ccgacgccga tctcaacggc cctcagcccg
      481 gccccagctg gcagcgcggc aactggcccc aggtgggagg cgcggctgaa aacgacctca
      541 cccaggcgct cgatcccacc atcctcaagc ccgccatcaa ggaagccgcc aagaaggccg
      601 gcgcgccctt gagcgaggcc gctgtggaag aggccgcggc cgattcgatc cgcgcccaga
      661 tgctgatccg cacctatcgc gttcgcgggc atctggccgc cgatctcgat ccgctgggcc
      721 tttccaagcg cgatatcccc gccgacctca cgccggaata ccacggcttt gtgggcgccg
      781 cgcttgatcg caaggtgttc ctgggcggca cgctgggcct gcagtgggcc acggtaaagg
      841 aaatcgtcgc catcctgcgc gccaattatt gcggcaaggt gggcctggaa tacatgcaca
      901 tcaccgatct tgaggaacgc aagttcctgc aggaccgcat ggaaggcgcc gacaagcaca
      961 tcgatttcac ccccaatggc aagaaggcca tcctgggcgc ggtgatccgc ggcgagcagt
     1021 acgagaagtt cctcggcaag aaatatgtcg gcaccaagcg cttcggcctg gatggcggcg
     1081 aatcgatgat cccggcgctg gaagcggtga tcaagtatgg cggccagctg ggcgtcaagg
     1141 agatcgtcta tggcatggcc catcgcggcc gcctcaacgt tctcgccaat gtgatggcca
     1201 agccctacaa ggtgatcttc cacgaattct cgggcggcag cgccaacccc gacgatgtcg
     1261 gcggctcggg cgatgtgaag tatcacctgg gcaccagcac cgaccgtgat ttcgacggca
     1321 tcaaggtgca catgagcctg atgcccaacc ccagccacct tgaaacggtg gacccggtgg
     1381 tgctgggcaa ggtgcgcgcc tatcaggtgt tccgcggcga tgtgggcgaa ggcaagcaca
     1441 agcaggtgct gccggtgctg atccacggcg atgccgcctt tgccggccag ggcatcgtgt
     1501 gggaatgctt cggcttctcg ggcgtgcatg gctataacac cggtggctgc atccacttcg
     1561 tcatcaacaa ccagatcggc ttcaccacca gcccgcagtt cagccgcggc tcgccctatc
     1621 cttcggatgt ggccaagggc gtgcaggcgc cgatcatcca tgtgaacggc gacgaccccg
     1681 aggccgtcac cttcgcctgc aagttcgcta tcgactatcg ccagaccttt ggccgcgata
     1741 tcgtgatcga catgtggtgc tatcgccgct tcggccataa cgagggcgac gagccttcgt
     1801 tcacccagcc gctgatgtat gccaagatcc gccagcacaa gcctgtctcg gagatctatg
     1861 ccgagcgcct gcagcgcgat ggcgtgatcg acggcggctt cgtgggcgag caggtggctg
     1921 ccttcaccgc ccatctcgaa gagcatttcg atgccgccaa gacctaccag gccaatgagg
     1981 ccgactggtt cggcggccgc tggtcgggct tcaaccggcc gatggacccg gaaaccgcgc
     2041 gccgcaacgt gcccaccggc atcgagggca agctgttcga cagcctgggc cgcacgctga
     2101 ccaccgttcc cgaccacctc accgtccaca agacgctggg ccgcgtgatc gacgccaagc
     2161 gcgagatgtt caccagcggc gagggcttcg actgggccac cggcgaggcg ctggcctttg
     2221 gcgcgctgct gtcggaaggc tatgcggtgc ggctgtcggg ccaggattcg ggccgcggca
     2281 cgttcagcca ccgccacgcg gtgtgggtgg accagaacga cgagcacaag ttcatcccgc
     2341 tttccaccgt gccccatggc cgcttcgagg tgtatgacag cccgctgtcg gaatatggcg
     2401 tgctgggctt cgaatatggc tatgccagcg ccgatcccaa gacgctggtg ctgtgggaag
     2461 cgcagttcgg cgatttcgcc aatggcgcgc agatcatgat cgaccagtat gtggcgtctt
     2521 cggaatcgaa gtggctgcgg gccaacggcc tcgtcatgct gctgccccat ggctatgaag
     2581 gccagggccc cgagcattcc tcggcccgcc tcgagcgcta tctgcagctg tgcgcgcagg
     2641 acaacatgca ggtgtgcaac atcaccacgc cggccaacta cttccacgtg ctgcgccgcc
     2701 agatgcaccg ccccttccgc aagccgctca tcatcatgag ccccaagagc ctgctgcgcc
     2761 acccgatggc caagtcgaag gcggccgatt tcatcggcga cggtcacttc atgcgcatcc
     2821 tctccgaccc gaccccggcc gaagatgcca agaccaagcg cgtggtgcta tgctcgggca
     2881 aggtggccta tgatctgatc gaggcgcgcg acgctgccag cctcgccgac acgcagatca
     2941 tccgcatcga gcagatctac cccttccccg gcgaaccgct ggccaagcgc ctggcacgca
     3001 tgggcgccct ggaagaggtg gtgtggtgcc aggaagagcc gaagaacaac ggctcgtggt
     3061 tcttcgtcga gccgttcatc gaggacgtgc tgaaggcggc caaggcacct gtgacgcgcg
     3121 cccgctatgc cggccgcgcc gcttctgcct cgcccgccac cggccttgcc aagcgccatg
     3181 ccgccgaaca ggccgccctg gtcgccgatg cgctgggcct gtcggtccgc tcggaaatcc
     3241 gccgcaagca aaaggcctga acgcctcgcc ccccgttcca aactgaagat tggaagtccc
     3301 agtcatgtct accgaagtca aagtccccac cctcggtgaa tcggtgagcg aggccacgat
     3361 cggccagtgg ttgaagcagc ccggcgaagc ggttgcgctc gacgagccca tcgccagcct
     3421 cgagaccgac aaggtcgcca tcgaggtcaa cgcgccggtt gccggcgtgc tgaccgccca
     3481 gctggccgaa gtcggcgcga cggtgacggt gagcgccatc atcgccacca tcgaggcggg
     3541 cgctgccgcg gccgcccccg ccgccaagcc ggccgcagcc ccggctgccc ctgccgccgt
     3601 ggccccggtg ccggtggccg ctgccgccga agcgaccgac cctgccgtgc tgagcccggc
     3661 cgtgcgccgc gccgtgctgg aatatggcat cgacccctcc accgtgaagg gcaccggcaa
     3721 ggatggccgc atcaccaagg aagacgtgat cgccgcggcg gccaacaagg cagcctcgcc
     3781 ggccgcggca gcctcggtgc cggccgcgcc cgccaacgag cgccgcgagg aacgcgtcaa
     3841 gatgacgcgc ctgcgccaga ccatcgccaa gcgcctgaag agcgcgcagg acaccgccgc
     3901 cctcctcacc accttcaacg atgtcgacat gtcggcggtg attgaagcgc gtgcccgcta
     3961 caaggacacg ttcgagaaga agcatggcgt gcgcctgggc ctgatgagct tcttcgccaa
     4021 ggcctcgtgc ctggcgctga aggacattcc ggcggtgaac gcccaggtcg atggcgacga
     4081 gatcgtctac cacgattatg tcgatctctc ggtcgccgtc tcggccccca acggcctcgt
     4141 cgtgccggtg gtgcgcaatg tcgacaagct gagctttgcc gagattgaaa aggcgatcgc
     4201 cggctatggc aagaaggcca aggatggcgc ccttaccatg gacgacatga agggcggcac
     4261 cttcaccatc tccaatggcg gcgtgttcgg cggcctgatg agcacgccca tcatcaaccc
     4321 gccccagtcg gccgtgctgg gcctgcaccg catcgaggac cgcccggtgg tccgcaatgg
     4381 cgagatcgtc atccgcccga tgatgtatat cgccctctcc tacgaccatc gcatcatcga
     4441 cggccgcgag gcggtgaccg ccctcaagac catcaaggaa gcgatcgagg atcccacccg
     4501 cctgctgatc gatctttaag gcgcggacga ggagcaagac catggctgaa tacgactacg
     4561 acgtcctcgt ccttggggcg ggccccggcg gttatgtggc tgccattcgc gcagcccagc
     4621 tgggcctgaa ggtggcctgc gccgaagggc gcgccaccct gggcggcacc tgcctcaatg
     4681 tgggctgcat tccctcgaag gcgctgctgc atggcagcga gctgttcgag gaagccgccc
     4741 acggcacgct cgccaagttc ggcgtgaaga cgggcccggt ggagctggac ctggcgaccc
     4801 tgcagggcga gaaggccagc gccgtgaagg agctgaccgg cggcatcgaa tacctcttca
     4861 agaagaacaa ggtggactgg ctgaagggct atggcacctt caagtctgcc catgaggtgg
     4921 atgtggccgg caaggtggtc agcgccaagc acgtgatcat cgccaccggt tcctcggtgg
     4981 cgccgctgcc gggcgtagcg gtggacaatg acgcgggcgt ggtggtcgat tccaccggtg
     5041 cgctggcgct ggaccgggtg ccgcagcacc tggtggtgat cggcggcggc gtgatcggcc
     5101 tggagctggg ctcggtgtgg cggcgcctgg gcgcgcaagt caccgtggtc gagttcatgg
     5161 accagctgct gcccggcatg gacggcgaag tgcgcaagga agcggccaag atcttcaaga
     5221 agcagggctt cgagctcaag ctgtcgacga aggtgacggg cgctgtggtg gaaggcggca
     5281 aggccaccct cacgctggag cccgccgctg gcggcgaggc caccacgctg gaagccgatg
     5341 ccgtgctggt ggcgattggc cgccggccga acaccgaagg gctggggctg gagaacatcg
     5401 gcctcgtgct caacaagcgc gggcagatcg agaccgacca tgacttccgc accgcggtgg
     5461 atggcgtgtg ggcgatcggc gatgtgatcc ccggcccgat gctggcccac aaggccgagg
     5521 acgagggcat tgccgcggcc gaaaacattg ccggcctcac cggcatcgtc aaccatgacg
     5581 tgatccccgg cgtggtctac accagccccg agatcgccgg cgtgggcctg acggaagaag
     5641 ccgccaagga acgcggggcc atcaaggtcg gcaagttccc catgctggcc aacagccgcg
     5701 ccaagaccaa ccgcgagccc gatggcttcg tcaaggtcat cgccgatgcc gcaacggacc
     5761 gcgtgctggg cgtgtggtgc attgcctcgg tggccggcac gatgatcgcc caggccgccc
     5821 aggcgatgga atttggcgcc accagcgaag acatcgccta tacctgccac gcacacccga
     5881 cccattcgga agcggtgaag gaagcggcga tggccgtgct gggcaagccg atccacatct
     5941 gaccggcaag ctgttgcagc gagaacgggc cggcagggtt tgcgccccgc cggcccgttt
     6001 tgtttgttgt gcctatctgg cgttcacgct gaacacccag ccctcgccat agcgccgcaa
     6061 ttgcccggca ggtacggcaa agccgccctc ccgcaggcgc tgggccactg cctcggccga
     6121 gggatattgc ccggcgacat gttccgtcca gaacagggtg gggcaggtgg gcgagaggcg
     6181 gcgggcggcg atcggcgtca aggcaaagcc gtggtgcgcg gccacccagc cataggcaaa
     6241 gggcaccacg gcgcggttgt cgggcggggg caggtccagc gtcggggcat tccagctggt
     6301 gttggcatag accagcgtgg ccgggcacgc tcgcgtggca gcggcaatgg cgcgggcggt
     6361 gccttcccag ctgggctggg cgatggtggc cacataatgg gcatgcagcg cccacaaggt
     6421 ggcaagaaac agcgccccat ccagcagggc tgccgccgcg ggccgcagcg cgcgggccag
     6481 cgcggcatag cccaccgcca gcgccagcgt cagcggcggg tggaggcaga cgagatagcg
     6541 gttgatgatg aaaggccggt gcaggtgcag cgccaccagc agcgccaccg ccagcgccac
     6601 accgcccgcc agcgtgagca ccaggctgcc ctcacccgag agccgccgcg cccgcagatc
     6661 gcgcagtgcc accagcgcca gccccgccag cccggccagg ctgagcagcc gattgccggc
     6721 cagattggcc agcacctcgt tttccaccgc ccagcgcgcg gccgaaaggc ccggcgggat
     6781 ccagaagctg gcggtgttgg cggccatggt gcgccattgc agcgccatgc tggcggcaaa
     6841 gggcagcacc gccagcacgc ccgccagcgc cagcgcccgc gccgccggcc agtcccgcgc
     6901 cagcagcagg cgcaggccaa aggcaagccc cagcgccccc atcaccacgc tggccaccag
     6961 atgcaccgac agcgccactg ccaacgccgc caccagcgcc gcccaggcgc ttcggctcac
     7021 ccgcgcaccc ggccgcgcca catcggccag cagcaccagc gccaccgcgc ccgacaccag
     7081 caccagcgca taggagcgca gttccgccac ccggttgatc gtctggtaat tgctggcaag
     7141 gcccagccca taggccagca gcaccagccg cagcggcgcc cgccgcaccg ccagccaggc
     7201 cagcgccccc atgcccccca gcaggaaggc cagattgacc agccggcgcg gccctacccc
     7261 gccctcgccc agcatgcccc catgcaactg gcccccatgc aactggccaa gcccgttgct
     7321 ggcccaggcc agcgcataga acagcggcgg atgattgtcg cgcagccaca ccggccaacc
     7381 ctgggcaaag gaaaggcccg gccgggtgac atagaaggtg tagaattcgt cataccaggc
     7441 cccgcgcgcg caaagggcgg ccagcgcggt ggccagcagg gcaaggccgg tggcccaggc
     7501 gatcagtttc tgacggtgaa gaagcagcca catgcgcctc ttgtggcgcg aacaggttga
     7561 tttttagaca atggggccaa gcccctcagc cggcgtagcg atcgatcacc gcccctgtgg
     7621 caggggctgc ggcatgcagc cgcgccagat gcagcgccag ctcttccagg ctgaagccct
     7681 tttcgaacac cagcccatag cgtggctggt ggcgccagca caccttggca tgcaccggcg
     7741 gaaagccttc cgcttccagc cgcacgggct ggcgcagcat cagccgctcg cccgtctcga
     7801 tgcaggcgcc ctgctgggaa atatcgcgca gcagcaccgg cccgtcgtgg ccatgggcca
     7861 ccagccgggc gatgcagccc agccgcatgc ggatctgccg cttgggatac tggtcgcgct
     7921 tgtcctcgat caggcggtgc acgtcgatat cgccaaggaa gcggaagccg gcatgatcgc
     7981 tggtctgcca caccagttcc atcggatagc gctcgccgct gcccagttcg agggcgatct
     8041 ggccctgggc cggcaggggg tggaacaggc gcaccttcac gcccgtgttc gaggcatcgc
     8101 gcagcacgca cagatattcg cgcccgtcga tcaccagctt ggccgtgcgc accagcaggg
     8161 tgaagcgctg ggcgccacga tgctctgccc ccgggggcag aagcgccccc gagtcagggc
     8221 cggccacagg gccagtcgtg ttccaggtcc attcttgcga caagcgcgtc acctagccaa
     8281 tcgtaacgat ccgggcagaa agagggcata cctcccccct tcatcccggc gattaccatg
     8341 cctgacaatg gtaaagcggt tgtaaaatat ttcctcggaa aacacctaaa tgtaaaatat
     8401 tcagggttaa tttggttcgt tggcgcaagt gggcgggcgc ccgggccgca gccctgcccg
     8461 ccccccgacc agcccttgcc tgtcaggcca cagcgccatg acaatgcttg tatttctggc
     8521 ccgagccgca ggggcacagg gcgttccggt tcagccccat gtcggcatag ggatcaacgc
     8581 cgggaatggc cggcggcccg gcctcgttct cgccggtgaa gggatcggtg tgggtggtga
     8641 ggaaatcggg cagttcgggc agcgccggct cgggcggggc gaattgcagc tcggcatggg
     8701 agaggatgcg cgtcacatcc tcgcggatgg tgtcgagcat gcgctcgaac aggccgaagg
     8761 cttcctgctt gtattcgttg atcggcgtct tttgcgcata ggcgcgcagg aacaccacct
     8821 ggcgcagcgc atccagcgtg gccagatgct ccttccagta gtggtccagc cgctccagca
     8881 ccacgctctt ttccacctgg cgccagatct cggcgttttc ggcctgcttg gcggccatgc
     8941 gctcgtcggc gaaggtggag atatgctgct ccagcgtgtc gggctcgatg ccatcctcgg
     9001 ccagccagtc gtccagcggg atttccagcg ccagcagttc cagcgcgcgc gccttcagcc
     9061 cctcggtgtc ccactgttcc ggataggaac catgcgggca agcggtggcc accagcgtgt
     9121 tcaccgtgtc gtggcgcatt tcggccacca catcgtccag cgtctcggca tccatgatgt
     9181 cggcgcgctg ctcgtagatg accttgcgct ggtcgttcat cacgtcatca tattcaacga
     9241 cctgcttgcg cacgtcatag ttgcgcgctt ccaccttctt ctgcgccgtc tcgatcgcct
     9301 tcgacagcca gcggctgccg atcgcctcgc catcggcaag gttggcgttc atcatgcgcg
     9361 agaacagcgt gtcggggccg aagatgcgca gcaggtcgtc ttccaggcac aggtagaact
     9421 tcgacaggcc cggatcgccc tggcggcccg aacggccgcg cagctggttg tcgatgcggc
     9481 ggctttcgtg gcgctcggtg ccaatcacgg ccaggccgcc ggcggccagc acctgctgct
     9541 tttccgcctc gatctcggcc ttgatgcgct cgatcgcggc atcgcgctcg gcgccttcgg
     9601 gggcatcctt cagctcgtcg ttgatgcgga attccaggtt gccgcccagc ttgatgtcgg
     9661 tgccgcggcc ggccatgttg gtggcgatgg tcaccgcgcc aaggcggccg gcctgcgcca
     9721 cgatatgcgc ttccatctcg tggaagcggg cgttcagcac attgtgcttc acctcttcct
     9781 tggtgaggaa ttcgctcagc agttccgatt tctcgatcga caccgtgccc accagcaccg
     9841 gctggccgat ctcgttcttc tcgcggatca gcttggcgat ggcagcgaat ttgtcatgcg
     9901 tgttcttgta gaactcgtct tcctcgtcga tccgctgcac cggcacgttg gtggggatgc
     9961 tcaccacgtt catcttgtag atgtcgaaga attcgggcgc ttcggtggcg gccgtgccgg
    10021 tcatgccgga aagcttggga tacatgcgga aatagttctg gaaggtgatc gaggccatgg
    10081 tctggttctc gggctcgatc ttcacgccct ccttggcttc caccgcctgg tgcagcccgt
    10141 tcgaccagcg ccggccatcc atcatgcggc cggtgaattc atcgatgatg acgaccttgc
    10201 cgtccttcac gatatagtcg atgtcgcgct tgaacatcac attggccttc agggcctgat
    10261 cgaggtggtg gacgacctgg gtgttctcga tgtcgtagag gttgctgccc accagcaggc
    10321 cggcattttc cagcagccgc tcggcctttt ccacgccctc ttcggtcagg gtgatgttct
    10381 tggccttctc gtcggcctcg taatcctcgg ccaccagatg gcgcacgatg gcatccaccg
    10441 ccacatagag ctcgctcttg tcgtcggtcg gccccgagat gatcagcggc gtgcgcgcct
    10501 cgtcgatcag gatcgaatcc acctcgtcga cgatggcgaa attgaacggg cggtgcacca
    10561 tctgctcgcg cgtgtgcttc atgttatcgc gcagataatc gaagcccagc tcgttgttgg
    10621 tggcataggt gatgtcggaa ttataggcct cgcggcgctg cacctcatcg aggttgggaa
    10681 cgatcacgcc cactgtcagc ccgaggaaat tgtagagcac gcccatcgtc tcggcatcgc
    10741 ggcgggccag atagtcgttc accgtgacga catgcacgcc cttgccttcc agcgcgttga
    10801 gatagaccgc cagcgtgccc accagcgtct tgccctcgcc ggtcttcatc tcggcgatct
    10861 cgccgcggtg tagcacgata ccgccgatca tctgcacatc gaaatggcgc atgcccatcg
    10921 cccgcaccgc cgcctcgcgc acggtggcaa aggcttcggg cagcaggctg tcgagggtcg
    10981 cgccctcggc gagcgccgcg cggaacttca ccgtctggcc ggccagttcg gcatcgctca
    11041 gcgcctgcat ggcaggctcg aaagcggcga tcttctgcac cagcttgtcg agcgacttga
    11101 cgtaacgatc gttggacgag ccgaacaggc tcttggcgag tttgccgaac atgggatgat
    11161 cctgtgtggg aaaaggcgtg agaaaggcgg catggcggcc agcaggccgg atggccgata
    11221 gcgccgggcg atcagccgcg ccggttcgcc aaaggccaaa aagcaggcaa atgcccgcgc
    11281 aaaaaagctt attcgcgggt gcgatcggcc cgcaggcgca ccgccggaat ggcaggggcg
    11341 gcaacagcgg gcagcggccg ggcagcggcc caggcgggct gctcgccagc caccgcgcgc
    11401 acggcggcca cggcctgcac gcgcgcggcc accaccgccg cccgcgccgc gcccagcgag
    11461 gcggccgcca ccgcgggcgc cgtgcccaca ggcatggcca tgcccgccgg cacctgcagc
    11521 aacaggccgg tcaacatcgc caaaagggca agcatcgcac gcttcataag gggcggatat
    11581 aggttggcaa aaggcgaagg tccagccctg ccggcgaatc cttgcccccg cctggccaac
    11641 aggctaaccc gccgccatgt ctcacgccat ttccccgctc gcctcgccct ttcccgcgct
    11701 tgcgccgatt gccggcgcca ccgcccgcat cgcccgcgcc ggctacaagg actggggccg
    11761 ctgcgacctc accttcgtgg agctggacga gggcaccagc gttgccggcg tgttcacccg
    11821 caacgtgtgc tgttcctccg aggtggagct ggggcggctg aacgtggcgc agggccaggc
    11881 ccgggcgctg atcgtcaacg ccggcaattc caatgccttc accggctatc gcgggcgcga
    11941 ggcggtggag cagatcatgg atcaggtggc cgcccacctt gcctgcccgc gcgagcaggt
    12001 gttcgtctcc tccaccggcg tgatcggcgt gccgctgccc aaggacaagg cgcgcgaagg
    12061 cgtggccgca gcgcttgcgg ccgccccctg caactgggaa gaagcggcca acaccatcgg
    12121 caccaccgac acctttgcca agggcgccag cgccaccgcc atcgttgacg ggcacaccgt
    12181 caccttctcg gcgatcatca aagggagcgg catgatcgcg ccggatatgg cgacgatgct
    12241 gggctatatc ttcaccgatg ccgccgtggc gcccgccttc ctgcaggcgc tgctctcccg
    12301 cgcggccgat gccaccttca actgcatcac ggtcgacagc gacacctcca ccagcgacac
    12361 ggtgctggcc tttgccaccg gcaaggccgg caatgcgccg atcactggcg ccgacagcgc
    12421 cggcgccgat gcctttgccg ccgcccttca cgatgtctgc cgccagctgg cgcagctggt
    12481 ggtgaaggat ggcgagggcg cgcagaaatt catcgccatc accgtcagcg gcgccgtctc
    12541 ggatgagagc gcgcgcaagg tgggcctggc cgtggccaat tcgccgctgg tgaaaaccgc
    12601 catcgccggc gaggatgcca attggggccg cgtggtggcg gccatcggca aggccggcca
    12661 accggccgac cgcgaccggc tgtccatcgg ctttggcggc gtgtgggccg cgcgcgatgg
    12721 ccagccgctg gccgattacg acgaggcgcc ggtggccgcc catctgaaaa cccgagagat
    12781 caccatcgag atcgacctgg gcctgggcga aggccgcgcc acggtgtgga cctgcgacct
    12841 cacccatggc tacatctcga tcaacgccga ctacaggagc tgatgccatg ctgacgatct
    12901 ggggccggct caattcgcac aatgtgaaga aggtggtgtg ggcggccggc gaggccggca
    12961 tcgcctggca gcgccgcgac atgggcggca gctttggcta cacgcccgcc tatctggcgc
    13021 ataaccccaa ccacctcgtg ccgatgatcg aggaggcaga tggcttcacc ctgtgggaat
    13081 cgaacgccat cctgcgctat ctggccagcg cccatgcgcc ccatctatgg gccggcgatg
    13141 cgcggcaaaa ggcccgggcc gacaaatggc tggactggca atttgccttt gccgatgccc
    13201 agcgcgatgg cttcctgcag ttggtgcgcg tggccgaggc ccagcgcgat gccgccaaaa
    13261 ttgccgccac ggcggccgcc accggccggc tgatggccat ggccgaggca gagctggccc
    13321 gccagccgtt cctttccggc gcggaattcg gcatcgccga catcgccatg ggcacttatg
    13381 cccacacctg gttcacgctg gggttcgacc gggcggaagg cggcgagaaa gcggaagtgc
    13441 cgcatgtgcg cgcctggcat gccgccctca tcgcccgccc ggcctttgcc gccatcgcca
    13501 tccccctcag ctgaaagccg cgtgatgacc gacagcctga ccgaagccgt ctaccatgtg
    13561 atggccgatg ccgcccggcg cgccattacc ccgcgctatc agcacctggg cgcgggcgag
    13621 atcatcgaga aggcggccga cgatctggtg accgtggccg accgcgagag cgaggcgatc
    13681 ctcgccgagg ggctggcgcg catcctgccc gaagcggcga tcgtgggcga agaggccgcc
    13741 tttgccgatc cggccatcct cggccggctg ggcgaggatc tgtgctggat catcgatccg
    13801 ctcgatggca cgcataattt cgcccatggc aagccgccct tcggcatcct catcgccctc
    13861 agccaaagcg gcgagacgat cggcggctgg atctatgatg tgctctcggg ccgcttctgc
    13921 catgcccgcc ggggcgaagg cgccttcatc gatggcgagc ccatccgcgc gcgcagcagt
    13981 ggcgaggaac cgcccatcgg cgccatcagc gtgctgtttg ccgatgccga gcggcgcgcg
    14041 gcgatcactg cccatgtcgc cccgcattac cggctggtgg acattccccg ctgcgcggcg
    14101 gaacaatatc cgcgccttgc cacaggcgtg aacgatgtct cgatcttcga gcgcacgctg
    14161 gcctgggacc atgccgcagg cgtgctgttc ctcaacgagg ccggcggcaa ggcggcccgc
    14221 ttcgatggca gcgcctatcg ggtggatgat gcccgcaagg ggctgatcgg cgcggcctcg
    14281 ccggcgctgt gggaggcgct ggccacccgc atggccgagc tctgagggaa aggggcgcgt
    14341 ggcggcgccc ctttggcctt cttcagataa cgctttcgat ccagcccttc agctggctct
    14401 tgggggcagc gcccagcttg gtggccaccg gctggccatt cttgaacagc accagatagg
    14461 ggatcgactg gacgccgaac tggctggcgg tgctggtgtt ttccatgatg tccaccttgg
    14521 cgatggtcac ctggccggcc agctcctcgg cgatctcttc cagcgccggg gcgatcatct
    14581 tacaggggcc gcaccaatcg gcccagaaat ccaccagcac gggcttgtcg gcgcccagca
    14641 catcggtggc aaagcttgca tcgttgacgg tgatagtggc catgggaaac tcctttgggg
    14701 gggttatcta ggtctgtggg ggcatttggc aagcgatcac agcgccaagc tttcctgcgc
    14761 cagccccaag cctgccttgt ggcgctccag cacatcgacc gggatctcga tcagccgggg
    14821 gctttgcgtg tagagcaggg cggcttccac cgggcggccg gggaaggctt cggccagcgc
    14881 cgcggcatag gcggccatct gccgcaagat cgccaccggc acctgatcca gccgggtggg
    14941 cgggcggcgg gcggatttga aatcgaccag gcggatgcgc ccctcctcca ccagcagccg
    15001 gtcgatcgtg ccggcgatca cctgcccggc caccaccgct gccaccggca cttcggcgag
    15061 cgctgccggg ctgaacagat ccgcccagcg cgcatcgcca atcaccgcca gcgcggtggc
    15121 ggcaatctcg gccctgaggc tggcttccac atcgcccgcc tgccgcgcca gccaggcttc
    15181 ggccagcggc gcgcgggcat cggccggcac cgcgcccagc cgttccagca gcttgtgcat
    15241 cagcgtgccg cgccgggccg cgcgggcgcg gtcggcgctg ttggccatgc cggtgggcgg
    15301 gttggcggaa tggtcctcgc ccagcgcaga aggggaaagc gggcgcggcg gcaccggctc
    15361 ttccgccggc gcgcgcagca gccagccggg cagcgccggg cccagcggca agctggccgc
    15421 cgcgccggcc cggcggatgg gggccgtggc cctggcgcca tattccagcc gccagcccca
    15481 cagcggatca tccagccgcc cctcgccgcc acccgcttcc gccacatcgg ccaggcgggc
    15541 aaaccagctg tcgtctgccg gcgccgtctc gcgccttgcc agcgcaccgc cgatgaacag
    15601 cgcttcctcg gcgcgcgtca tcgccacata gagcaggcgc cagtgctcct cgctctcggc
    15661 cttcagcgcg gcggcatcgg cggccttgat cggccccagc cgctcgccct tgcgcaggcg
    15721 cggcaagggc atctggcgcg gctctccccc gcccagcagc gcggccggat cgtccacccg
    15781 cgccgcgccc tcgcggccca ggcgcggatc gcccgccgca tcggcgagga taacgatcgg
    15841 cgcctgcaac cccttggagc catgcaccgt catcacccgc acacggtcgc ccgaattgcc
    15901 ggcctcgcgc ttcagctcgc cctcggcgcc atcgaaccag tcgaggaaac cggccaggct
    15961 ggaggtggcg ctggcggcat gggcaagcgc tgcattgacc agctcctcga tcggatcggc
    16021 cgcctctgcc cccagccgcg tcagcagccg tgcccgcccc gcccatggcc ccaccagcaa
    16081 ccattgcagc agcgcgggca cggtgtcgaa atcggcgcgg gccagcagcg gggccagctg
    16141 ttcgcgcacg gccccagcct cggcatgggg gctgcggcgg atatgatccc acagccgcgt
    16201 gccgcgcggg cggtggccat gggccagcaa ttgcgcctgg ctccagccga ccagcgggct
    16261 gaccagcagg ctggccaggt tgagatcatc cagcggctgg gcggcaaaac gcagcgccgc
    16321 caccagatcg cgcacggcaa ggggcgctgc cagccgcagc ctgtccacgc cggccaccgg
    16381 cacgcccgcg gcatgcagcc gcgccacgat cagcccggcc agatcccggc gcttcctcac
    16441 cagcaccatg atgtcgcccg gagtggccgc gcgcgcctcg ccctccttct cgcccttcac
    16501 cagcgcgaag ccatcggcca gccaggcctt cacctgcgcg gcaaggcgct cggccatctg
    16561 ccggtcggtg cgcgaaagcc agttctgcgc cacggccggg ccctcgtcct cgccggcggg
    16621 ctcatcttcc tcttcctcgt ccgggcggcc gcccaccggc gcccacagcg ccacatagcc
    16681 ggggcgcggc tggccctgat gcggctctgc cgggcgatcg aggccgaatt gcgccgcgcc
    16741 catccgcgcc atcgccagat cgacgaaatc gagcaccggc cttgccgtgc ggaaggagcg
    16801 ctccagcccc agttccagca gcccctcggc ctcctcgccg ccgcgctgct ggcgggcgtt
    16861 ggcggtggcg gcggccatct cgcccttcac ccaggcgccg gcgcgagcga aattctccgg
    16921 gctggtgccc tgaaagccga agatcgcctg cttgtaatcg cccaccacga acagcgtgcg
    16981 cggcggcggc ttgcccccgg gcccgcccca ggcgctggtg cgcgccccct cgcccgagaa
    17041 gaattcgccc accatcgcgc ggatgatccg ccattgcgcg gcattggtat cctgcgcctc
    17101 gtccaccagc acatgatcga tccgccggtc gagcttgtag cggatccagt cggccatttc
    17161 tgcgcgtgag aggaggtctg cggccagctg gatctggtcg tcgaaatcga tgacgccctc
    17221 gcgcgccttg gccgcatccc acgccagcgc atagcgccgc cccacttcca gcgccggggc
    17281 cagccagccg gccagcgcca gcagcgcccg ctgctcgcgg atggcattga tgcgctcgcc
    17341 cacgcggtag caatattcgc cataatcggg cgctatcttc accagcgagg ccagatagga
    17401 aggctcgtcc ttctcggtga acagcgcctt gtagagcagg tccagccccg ccagccgggc
    17461 ctcgggcggg gcggccagcc agtcgccgat ggcattggcc ttcttctcgg cgttggcggt
    17521 gaggttgccc ttcttgtcgg tccagccctt gcaggcggcc agcagttcgc gcagggaacg
    17581 ggtgtcgaag gcctcgtcgg cgcacagggc ggcaaggcct gccgcatcgg catcggccgg
    17641 caggcccagc acgcgggcga catggggcgc cagcggcggc acccagcagc ccggcccgaa
    17701 ccacagcgag cgggcagagg cgcaggccat cagccagcct tccacgccat ccagccccag
    17761 ccgcagcgcc agcgcttcca ccgccgccag catcgccgca tcgcccccac tttcggcatc
    17821 gcgcagcaga tcggccagca cctgccgggc cagcagccgc ttctgctggt cttccagcgg
    17881 gcgggcgccg ccggccagcc ccgcctcgcg cgggaaggcg gccagcagcc attgcgagaa
    17941 ggcatggatc gtgtcgatcc gaagcccccc gcccgggcaa tcgagcacgg cggcaaacag
    18001 cgtgcgggcg cgcgcctgcg tctccgggcc gatatcggcg ccaatcgctt ccagctccat
    18061 cgccaggcgc accggatcgg cgcgcaccca accggccagg cggctgtgga tgcgctctgc
    18121 catttcggcc gcgcccgcct tggtgaaggt gaggcacagg atctgctcgg gccgcacttc
    18181 gggctgcagc agcaggcgca gcacgcgggc ggagagcacc tgcgtcttgc cggtgccggc
    18241 gctggccgac agccacaccg tgcgcgccgg atccaccgcc cgcatctggt tgccggccag
    18301 cggatgcggc cttagctggg tgccgctcat gcagcgcccc cttccagcgt gccaatccat
    18361 tcatctaggc gcatcagctg gtcataatca ttgtagccac cgatatcggg gttgagccgg
    18421 gcggtgaagg gctctgcccc cagcagccag cgggcgatcg cctcgcgcag gaagcgctct
    18481 gtctcgggca ggaaatcggc acgcgggatg ccgctgcgct tgcgcccctc catgatcggc
    18541 tcctcgcgcc agccaaagcc atcgcccccc ttgcgcgcgc tcagcgacca gtattcgaag
    18601 gcctccgcct cgccgccaat gcccttgaag ccgccgcctt cggcaatcag gccaatcagg
    18661 cccagctgca aggcaaagcc ttcggccacc tgcttgccgg aagggggctt gccggtcttg
    18721 tagtcaacga tggcgagcgg gccttcgggc aagcggtcga tacggtcggc gcggccgtgg
    18781 atgcggatgc catcgacgcg gatctcgccg cggatttcgg tggccagcac ctgccgcccc
    18841 tcgcccttca ggcgcagcag ctcggcatcg atccattcca gcgcggccag caggcgcggg
    18901 cgccacaggc tgcgggtgag cggatgggcg ttcagcctgt cgagcatcgc cgcgccctcc
    18961 tgtgccagcc cgcccaccgg cgcgcccgcc cggtgccagg catccagcac gccatgcacc
    19021 gccgtgccgc gccaggcggg cgtgggcggg ctgtcgagcg gatcgagccg ggcgaggccg
    19081 aggatcgcct gcgcatagaa ctggtaagga tctgcccgca gccggtcgag cgcggtg
//
LOCUS       DASRYE010000112         9875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_57570,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000112 DASRYE010000000
VERSION     DASRYE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57570"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFF98_06195"
     CDS             <1..575
                     /locus_tag="VFF98_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HZU63254.1"
                     /translation="GDYAETEQAIQRLLAEAQGRAPVPGGTVAVAGAGAEQAASGAAQ
                     SPETYIGLARAERFAAQPAAVADRPADYRLMDLLPDGWGLSGRWQVGAEQARLLAPGG
                     AIAYRFEARDLHLVMGPGAGGHPVRFRVTIDGRAPGADHGADTDAEGRGTIDGQRLYQ
                     LVRMAAGAGDHRFLITFLDAGVEAFSFTFG"
     gene            complement(582..1442)
                     /locus_tag="VFF98_06200"
     CDS             complement(582..1442)
                     /locus_tag="VFF98_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HZU63255.1"
                     /translation="MALEPGKKAGKRSAEESGRQAQARVPVELRHQHMMQAFAEQGFI
                     SVIDMAELIGVSTMTIRRDLDLLEQAGKVMRIHGGAVLAAQKEDSETERKEDIFDRRL
                     AVNAPAKRAIARAVADLVGADPAIQSIGLDTGTTVLTAAHLLMGGKGLRFVTNNLRAA
                     LLLSGGESRVYMLGGEVRVPELSVVGPGAIKALQNQYLDVALIGVSGLDAQGLYDFSP
                     EDTEVKTVLMDNASKVVVVCDASKFERRALARIAPLERIHVLVSEAAPPPALAAALAA
                     AGVQVVVAGS"
     gene            complement(1539..3041)
                     /locus_tag="VFF98_06205"
     CDS             complement(1539..3041)
                     /locus_tag="VFF98_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZU63256.1"
                     /translation="MPSYPRCVIGLDIGTTSTIGMMIALPDQVLALASREVELRSPHP
                     GWAEEDPAQWWANVCAITQELLATAGLPPEAVAAVGVTGMCPAMVLLDEAGAVLRPSI
                     QQSDGRCSAEVAELRGEMDEAAFLARAGNGINQQLIAPRLRWIERHEPAIFARIATVM
                     GSYDYINWRLTGVRAIEQNWALEAGFTDVASGALAEDLIALGHIAPHQLPGRCLSHAR
                     LGVVTPTAAAATGLAAGTPVIGGAVDMIASALGAGVVHPGDVLIKLGGAVDVLTASTH
                     ARPDPRLFLDYHLVPGLFMPNGCMSTGGSALNWFVRTLARAEAQEAKARGLSPHALLD
                     ELAAARPAGCEGLRVLPYFLGEKTPLHDPFARGVFEGLTLSHDIGHLWRALLEAYAYA
                     IVHHIEVMRDIGHETTRFLVSDGGAASQVWLQIIADCLQQPLLRLKGHPGSALGAAWA
                     AAIGAGAESDWAGIARFTTQEPSVQPNPAHAAIYRNGYQAFRALHTRLYM"
     gene            complement(3063..3863)
                     /locus_tag="VFF98_06210"
     CDS             complement(3063..3863)
                     /locus_tag="VFF98_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HZU63257.1"
                     /translation="MIFDFLGDKRKAVIGMAHIGALPGSPNYDADGGMDRLIEDVLAD
                     IRALQQGGVDAIMFGNENDRPYLLKATPESIAAMTAIVTAARAELTCPFGVNYLWDPV
                     ASVAIGVATGARFIREIMTGLFASDMGLWCPDGASAARLRRQLGRADMKMLFNINAEF
                     AHSLDQRPIAQRAKSAVFSAMADAILVSGPMTGQSLDASDLRSVVEAVPGTPVFANTG
                     VNIDNVGDILSLASGVIVGTHFKVDGNTWNAVDGARVSRFMDVVEKLR"
     gene            4035..4826
                     /locus_tag="VFF98_06215"
     CDS             4035..4826
                     /locus_tag="VFF98_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63258.1"
                     /translation="MIPHALEPQDFAGKRALITGGATGIGLAAARALARRGAQVAIAD
                     IDAGAAAAAAAELGSSALAVAMDVRERASVEAGFAAALAGLGGCDMLVANAGVSTMQH
                     ALELTDAEWDFNFAVNTRGVFLTNQIAARHFRAAGGGVIVNTASLAAKVGAPLLAHYS
                     ASKFAVLGWTQALARELAPAGIRVNAVCPGFVNTGMQTREVAWEAELRGLSAERVLAE
                     YVAQTPLGRLETPEDVADVIVFLCSPAARFMTGQGVNVTGGVYMG"
     gene            complement(4814..6022)
                     /locus_tag="VFF98_06220"
     CDS             complement(4814..6022)
                     /locus_tag="VFF98_06220"
                     /inference="COORDINATES: protein motif:HMM:NF004636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU63259.1"
                     /translation="MTDTAPTLMPPELTLRHCRLPEWQGLFDVALANGRIAAITPSEE
                     APPAGPLRDLGGWLLLPSLVNGHIHLDKTFLGAPWRSHVPGASIAARIAAEAQERSLV
                     PLPLAARARHLAERSLAWGVTALRSHVDVDEAVGLAHVEALLALREELAGRIDIQLVA
                     FPQGGLSGRVPALMDQALALGVEVVGGLDPAGIDGDIEAHLGTVFGLAEKHGRRVDIH
                     LHDPGTLGAFELRRIAAHTRARGMAGQVAVSHAFALGMLAPEEAAATADALAEAGVAI
                     LTNGPGADAMPPLALLMDAGVRVFAGSDNVRDAWSPYGDGDMLRRASLIGYLADWRED
                     EGLVAAYGLASNEARAALGLPPVALSPGMPADLIALPATDIPEAVADPPPARLVLRAG
                     RVIAPAVQPI"
     gene            complement(6015..7097)
                     /locus_tag="VFF98_06225"
     CDS             complement(6015..7097)
                     /locus_tag="VFF98_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HZU63260.1"
                     /translation="MPYTPQNLSAAETEEARRTAARLIAHYREDSAEYPAELANHLPM
                     LLPILAQLGASSQRLEEYATLYRQIRRQPAMPRPLAPFTPADWQEGLGDRRREADYRR
                     FFAGEVARLGPEGAVRAYLPRLMQAAGSSAFHPLMRLAFGILRSDADEIAIALGFWAT
                     TELPLRNEPQGPQGQESPAQLLDFMRQSPAFSAIDIPATAPLWHWMRGVGGMDAFAPL
                     LGRLSPAPETLDAVGAAGLALFAAAPGSLEAVHAMTGGWWVRVVGQWLDAPADLARFF
                     WQAVLAVYPKTGMPALPPAEELAALRAAPAPPRAALAAAALACDPWGDDEEHHPSVVF
                     TAFQEYDRTGDRLYLNAAAKRMGLLD"
     gene            7255..8349
                     /locus_tag="VFF98_06230"
     CDS             7255..8349
                     /locus_tag="VFF98_06230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU63261.1"
                     /translation="MPSFPGPRAWLQAAALLLAGCHPAPSEPPHAPEPRAAGPARCAA
                     NRAAGPITFLTSAVFLPTTGILDVVAAQRLGYFDALCLDVRIRPGGSNAQLVSAGTAQ
                     IAGLGDAASALLAMDRGANLTGIATYGNISAVELVALRDAPEGSAPIRQLADLKGRIV
                     GYKVAVAPQVIAMLIRAGVSPDAVKFVSVPFNPEILAKGQVAAMIGYASNEPRALEAG
                     GYALSEWNPERFGVHSTFNVLVANRDFAAAHPQAVADFLRASLRALAWVRASDAHLDT
                     ALGFERDRSPAGFDLARARARWRYEEQMIAASQPAGSVPGENVPAGWQADARLLADAR
                     LTHGPAPARPFDNRFIQAIYKGTQLLWPAP"
     gene            8334..9377
                     /locus_tag="VFF98_06235"
     CDS             8334..9377
                     /locus_tag="VFF98_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZU63262.1"
                     /translation="MASTMKAWVQTGFGGPETRVLQEVPVPRPGAGEVLVKVAFCALN
                     RLDILQRRARLIPVMAPPHIAGMDFAGEVVAAAGPEGAALVGRQVVVDPVVTCGVCDR
                     CRAGLAVYCRQFRTCGSSRAGGLAEYVAVPAANCIVVDGAAARLAELACVPVASVTAW
                     HGLLGAGGLQPGETVVVPGAGSGLGIAGIQIARARGCRVITTVAGPAKLAQAAALGAD
                     LVIDRLAEDWVSAVQGFTDGAGADLVWDHIGGPFLQQAIDACRVNGRIVMSGTTAGNQ
                     STITNTSLFHWGKAIIGHGGYTPAEMRATVAAYASGGMRVVIDSEWAFADLPAAEQRL
                     ESGDFFGKVLVCL"
     gene            9368..>9875
                     /locus_tag="VFF98_06240"
     CDS             9368..>9875
                     /locus_tag="VFF98_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU63263.1"
                     /translation="MSLGGVPAIAVSGLAKSYRREGVPVPALAPTDLVVEEGRFVTLF
                     GPSGCGKSTLLRLVAGLEVPDAGEVRLFGRSPAETARAKALSWAPQSPALLPWLTIRQ
                     NLALPQRLNRRADRHPDPARQPRPVEEVLEEVGLAAFADARPAQLSGGMRQRAALARA
                     FVVGAPLMF"
BASE COUNT         1492 a         3361 c         3500 g         1522 t
ORIGIN      
        1 agggcgatta cgccgagacc gagcaggcca tccagcgcct gctggccgag gcgcaggggc
       61 gcgcgccggt gcccggcggc accgtggccg tggccggcgc cggcgctgaa caggccgcca
      121 gcggcgcggc gcaatcgccc gaaacctata ttggcctggc ccgcgccgaa cgctttgccg
      181 cccagccggc ggccgtggcc gatcgcccgg ccgattaccg gctgatggac ctgctgcctg
      241 atggctgggg gctttctggc cgctggcagg tgggggcaga gcaggcgcgg ctgctggccc
      301 cgggcggcgc catcgcctat cgcttcgagg cgcgcgacct gcatctggtg atggggccgg
      361 gggcgggcgg ccacccggtg cgctttcgcg taaccatcga cggccgcgcc cccggcgccg
      421 accatggcgc cgacaccgat gccgaagggc gcggcaccat cgatggccag cgcctttacc
      481 agctggtgcg catggccgcc ggcgccggcg accaccgctt cctcatcact ttcctcgacg
      541 ccggcgtgga agccttcagc ttcacctttg gctgaggccg gctagctgcc ggccaccacc
      601 acctgcacgc cggccgcggc cagggcggcg gccagggcgg gcgggggcgc ggcctcgctc
      661 accagcacgt ggatccgttc cagcggggcg atgcgggcca gcgcccgccg ctcgaacttg
      721 gaggcatcgc acaccactac caccttgctg gcattgtcca tcagcacggt cttcacctcg
      781 gtgtcctcgg gcgagaaatc gtagagcccc tgcgcatcga ggccggaaac gccgatcagc
      841 gccacatcga gatactggtt ctgcagcgcc ttgatggcgc ccggccccac caccgaaagc
      901 tctggcacgc gcacttcgcc gcccagcatg tacacccggc tttccccgcc ggaaagcagc
      961 agtgcggcgc gcaggttgtt ggtgacaaag cgcaggccct tgccgcccat cagcagatgc
     1021 gcagcggtca gcacggtggt gccggtgtcg agcccgatcg actggatggc cggatccgcg
     1081 ccgacaagat ccgccactgc ccgcgcgatc gcccgcttgg ccggtgcgtt aacggccagg
     1141 cgccggtcga agatgtcttc cttgcgctcg gtctcgctgt cttccttctg cgcggccagc
     1201 accgcgccgc catggatgcg catcaccttg ccggcctgct ccagcaggtc gagatcgcgg
     1261 cggatggtca tggtcgacac gccgatcagc tcggccatgt cgatcaccga gatgaagccc
     1321 tgttcggcaa aggcctgcat catgtgctgg tggcgcaatt ccaccggcac gcgggcctgg
     1381 gcctgcctgc cgctttcctc cgccgatcgc ttgcccgcct tcttgcccgg ttccaacgcc
     1441 atcgtcgcgc cgcgcgccat cctgctgtca ctttcctgtt atcgccgcgc gccgggccgg
     1501 caaaaaccga cgacaacgca caaagtttgt tccctttatc acatatacag ccgggtatga
     1561 agagcgcgaa aggcctgata gccatttcga tagatcgcgg catgggcggg gttgggctgc
     1621 acgctgggct cttgcgtggt gaagcgggcg atgccggccc aatcgctttc agccccggcg
     1681 ccgatggccg ctgcccaggc cgcccccaac gccgagcccg ggtgcccctt cagccgcagc
     1741 agaggctgtt gcaggcaatc ggcgatgatc tgcagccaca cctggctggc cgcgcccccg
     1801 tccgacacga ggaaacgcgt ggtttcatgg ccgatatcgc gcatcacctc gatatggtgg
     1861 acgatggcat aggcataggc ttccagcagc gcgcgccaca ggtggccgat atcatgcgag
     1921 agggtgagcc cctcgaacac cccgcgtgcg aagggatcgt gcagcggggt cttctcgccc
     1981 aggaaatagg gcagcacgcg caagccttcg cagccggccg ggcgggcggc ggccagttca
     2041 tccagcaggg catggggcga aaggccgcgc gccttggctt cctgcgcctc ggcccgggcc
     2101 agcgtgcgca cgaaccagtt gagcgccgag ccgccggtgg acatgcagcc attgggcatg
     2161 aacagccccg gcaccaggtg ataatcgagg aacaggcgcg gatcgggccg ggcgtgggtg
     2221 gaagcggtga gcacatccac ggcgcccccc agcttgatca gcacatcgcc cggatgcaca
     2281 acgccggccc ccagcgccga ggcgatcatg tcgaccgcgc cgccgatcac cggcgtgccg
     2341 gcggcaaggc cggtggctgc cgccgctgtc ggcgtgacca cacccagccg cgcgtgcgac
     2401 aggcagcgcc cgggcagctg atgcggggca atatggccaa gggcgatcag atcctcggcc
     2461 agcgcgccgc tcgccacatc ggtgaagccg gcttccagcg cccagttctg ctcgatcgcc
     2521 cgcacgcccg tcaggcgcca gttgatatag tcatacgagc ccatcaccgt ggcgatgcgg
     2581 gcgaagatgg ccggctcgtg ccgctctatc cagcgcaggc gcggggcgat cagctgctgg
     2641 ttgatgccat tgccggcgcg ggcgagaaac gccgcctcgt ccatctcgcc gcgcaattcg
     2701 gccacttcgg cagagcagcg cccgtcgctt tgctggatgc tggggcgcag caccgcgccg
     2761 gcctcgtcca gcagcaccat ggccgggcac atgccggtca cgcccaccgc cgccaccgct
     2821 tcgggcggca ggccggcggt ggccagcagc tcctgcgtga tggcgcagac attggcccac
     2881 cattgcgccg gatcctcctc cgcccagccc gggtggggcg agcgcagctc cacctcgcgg
     2941 ctggccagcg ccagcacctg atcgggcagg gcgatcatca tgccgatggt ggaagtggtg
     3001 ccgatgtcga ggccgatcac gcagcgcggg taagagggca tggggccggg catccttatg
     3061 ctttagcgca gtttttccac cacatccata aaccgggaaa cgcgcgcgcc atccaccgcg
     3121 ttccaggtgt tgccatccac cttgaaatgg gtgccgacga tgacgccgct ggccagcgag
     3181 aggatgtcgc cgacattgtc gatattgacg ccggtgttgg cgaaaacggg ggtgcccggc
     3241 accgcctcga ccacgctgcg caggtccgat gcgtcgagcg actggccggt catcggcccg
     3301 gagacgagga tggcatcggc catggccgag aacaccgcgc ttttggcgcg ctgggcaatc
     3361 ggccgctggt cgagcgaatg ggcaaattcg gcgttgatgt tgaacagcat cttcatgtcg
     3421 gcccggccca actggcggcg cagccgcgcg gccgaggcgc catccgggca ccacaggccc
     3481 atgtcggagg cgaacaggcc ggtcatgatc tcgcggatga agcgggcgcc ggtggccacg
     3541 ccgatcgcca cgctggccac cggatcccac agatagttga cgccgaaggg gcaggtgagt
     3601 tcggccctgg cggcggtgac gatggcggtc atcgcggcga tactttcggg ggtggccttc
     3661 agcaggtatg ggcgatcgtt ttcgtttccg aacatgatgg catccacgcc accctgctgc
     3721 agcgccctga tatcggccag aacatcctcg atcagccgat ccatgccgcc atcggcatca
     3781 taattggggc tgccgggcag ggcgccgata tgggccatgc cgataaccgc cttgcgcttg
     3841 tcgcccagaa agtcgaaaat cataggggga atcctctgct tggggcgcgg tggccagcat
     3901 ggccggcgca tccggacagg agacgtgtct acaacaagtc aaaaacgaac acaatctaac
     3961 aaataatgtt gcacatcact cgtaaggtga tcacaatccg ctcgatccgc cctcttgatg
     4021 cacaggtgcc tgccatgatc ccccatgccc ttgaacccca ggattttgcc ggaaagcgcg
     4081 cgttgatcac cggcggggcc accggcattg gcctggccgc tgcccgcgcg ctggctcgcc
     4141 gcggcgcgca ggtggcgatt gccgatatcg atgccggggc tgctgctgcc gctgcggccg
     4201 aactgggcag cagcgcgctg gccgtggcga tggacgtgcg cgaacgcgca agcgtggaag
     4261 ccggctttgc cgccgcgctg gcagggctgg gtggttgcga catgctcgtg gccaatgccg
     4321 gcgtttccac catgcagcac gcgctggagc tgaccgatgc ggaatgggat ttcaacttcg
     4381 ccgtcaacac ccgcggcgtg ttcctgacga accagattgc cgcgcgccat ttccgggccg
     4441 cgggcggcgg ggtcatcgtc aacaccgcat cgctggcggc caaggtggga gcgcctctgc
     4501 tggcccatta ttccgccagc aaattcgcgg tgctgggctg gacgcaggcg ctggcccgcg
     4561 aactggcgcc ggccggcatc cgggtgaatg cggtgtgccc gggctttgtg aacaccggca
     4621 tgcaaacccg cgaagtggcc tgggaagccg agctgcgcgg gctgagcgcg gagcgggtgc
     4681 tggccgaata tgtggcgcaa accccgctgg gccggctgga aacgcccgag gatgtggctg
     4741 atgtcattgt gttcctctgt tcgccggcgg cccgcttcat gaccgggcag ggggtgaatg
     4801 tgacgggcgg cgtctatatg ggctgaacgg cgggggcgat cacccggccg gcgcgcagca
     4861 ccagccgcgc cggcggcgga tcggccacgg cttcgggaat atcggtggcg ggcagggcga
     4921 tgaggtctgc cggcatgccc gggctcagcg ctaccggtgg caggcccagc gccgcgcgcg
     4981 cctcgttgct ggccaggcca taggcggcca ccagcccctc gtcctcgcgc cagtccgcca
     5041 gatagccgat caggctggcg cggcgcagca tgtcgccatc gccatagggc gaccaagcgt
     5101 cgcgcacatt gtcgctgccg gcaaacacgc gcacgccggc atccatcaac agggccagcg
     5161 gcggcatcgc atcggcgccg gggccgttgg taaggatggc cacgcccgcc tcggccaagg
     5221 cgtcggccgt ggcggcggct tcctcgggcg ccagcatgcc cagcgcgaag gcatggctga
     5281 ccgccacctg cccggccatg ccgcgcgccc gggtgtgggc ggcaatgcgc cgcagctcga
     5341 aagcgcccag cgtgccggga tcgtgcaggt ggatatccac ccgccgcccg tgcttttccg
     5401 ccaggccaaa caccgtgccc agatgggcct cgatatcgcc atcgatgccg gccggatcga
     5461 ggccgcccac cacctccacc cccagcgcca gcgcctgatc catcaacgcg ggcacccggc
     5521 cgctgaggcc cccttgcgga aaggcgacga gctggatgtc gatccgcccg gccagttcct
     5581 cgcgcagggc cagcagcgcc tcgacatggg ccagccccac cgcttcatcc acatcgacat
     5641 gcgagcgcag cgcggtcacg ccccaggcca gcgagcgttc ggccagatgg cgggcccgcg
     5701 cggccagcgg cagcggcacc aggctgcgct cctgcgcttc ggcggcgatg cgcgcggcaa
     5761 tgctggcgcc cggcacatgg ctgcgccagg gcgcgccaag gaaggtcttg tcgaggtgga
     5821 tatggccgtt gacgaggctg ggcagcagca gccacccgcc cagatcccgc agcgggccgg
     5881 ccggcggcgc ctcttcggat ggcgtgatgg cggcgatgcg gccattggcc agcgccacat
     5941 cgaacagccc ctgccattcc ggcaggcggc agtggcgcaa ggtcagctcg ggcggcatca
     6001 gcgtgggggc ggtgtcagtc aagcaaaccc atccgtttgg cagcagcgtt gagatagagc
     6061 cggtcgccgg tgcgatcata ttcctgaaag gcggtgaaca ccacgctggg gtggtgttcc
     6121 tcgtcatcgc cccaggggtc gcaggcaagg gcggcggcgg caagggcggc gcgcggcggg
     6181 gcaggggcag cgcgcaaggc ggccagttcc tcggccgggg gcagggcggg catgccggtc
     6241 ttggggtaga ccgccagcac cgcctgccag aagaaacggg ccagatcagc cggcgcatcc
     6301 agccactggc ccaccacccg cacccaccag ccgccggtca tcgcatgcac cgcttccagc
     6361 gatccgggcg ccgcggcaaa cagcgccagc cctgccgcgc ccaccgcatc cagcgtttcg
     6421 ggcgcgggcg acaggcggcc cagcagcggg gcaaaggcat ccatgccgcc caccccgcgc
     6481 atccagtgcc acagcggcgc ggtggcgggg atgtcgatcg cgctgaaggc ggggctctgg
     6541 cgcatgaaat cgagcagctg ggcggggctt tcctggccct gggggccttg cggctcgttg
     6601 cgcaggggca gttcggtggt ggcccagaag cccagtgcga tggcgatttc gtccgcatcc
     6661 gaacgcaata tgccaaaggc caggcgcatc agcgggtgga aggcgctgct gccggcggcc
     6721 tgcatcagcc gcggcagata ggcgcgcacg gcgccttccg ggcccagccg cgccacctcg
     6781 ccggcaaaga agcggcgata gtcggcctcg cgccggcgat cgcccagccc ttcctgccag
     6841 tctgccggcg tgaacggggc gagcggccgg ggcatggcgg gctggcggcg tatctgcctg
     6901 taaagcgttg catattcttc cagccgctgg gacgatgcgc ccagctgcgc gagaatcggc
     6961 agcagcatgg gcaaatggtt ggccagctct gcgggatatt cggcgctgtc ctcgcggtaa
     7021 tgggcgatga ggcgcgcggc ggtgcggcgg gcctcttcgg tttcggcggc agacaggttt
     7081 tgcggcgtgt agggcataga atggggatcc gcccgttgat agggaaggac agggaatgcg
     7141 cacatagtaa tgtttgtttg aagtttgaca atgtgcatta ttgtccgata tcaatcgcaa
     7201 tcgaaaacag cttcctcccg ctcaaggtgc cgcttgctgc ctatgttgct gcccatgcct
     7261 tcttttcctg gcccacgcgc atggttgcag gcggccgcgc tgttgctggc cggctgccat
     7321 cccgccccgt cggagccgcc ccatgcgcca gagccgcgcg cggccggccc ggcgcgctgc
     7381 gcggcgaacc gggcggcggg tccaatcact ttcctcacct cggcggtgtt cttgcccacc
     7441 accggcattc tcgatgtagt ggcggcgcaa aggctgggct atttcgatgc gctgtgcctg
     7501 gatgtgcgca ttcgcccggg cggcagcaac gcccagctgg tgagcgccgg caccgcccag
     7561 atcgccggcc ttggcgatgc cgccagcgcg ctgctggcga tggaccgggg cgccaacctc
     7621 accggcattg ccacctatgg caacatcagc gcggtggagc tggtggcgct gagggatgcg
     7681 ccagagggca gcgcgccgat ccgccagctg gccgatctga agggccgcat cgtgggctac
     7741 aaggtggcgg tggcgccgca ggtgatcgcc atgctgatcc gcgcgggcgt gtcgcccgat
     7801 gcggtgaaat tcgtctcggt gccgttcaac ccggagatcc tcgccaaggg gcaggtggcc
     7861 gcgatgatcg gctatgcctc gaacgagccg cgcgcgctgg aggcaggcgg ttatgccctc
     7921 agcgaatgga acccggagcg ctttggcgtg cattccacct tcaacgtgct ggtggccaac
     7981 cgcgattttg ccgccgcgca cccgcaggcg gtggctgatt tcctacgcgc cagcctgcgc
     8041 gcgctggcct gggtgcgcgc atcggacgcg catctggata cagcgctggg cttcgagcgc
     8101 gatcgctcgc cggccggttt cgacctcgcc cgcgcccgcg cccgctggcg ctatgaggag
     8161 cagatgatcg ccgccagcca gcctgccggc agcgtgccgg gcgaaaatgt gccggccggc
     8221 tggcaggccg atgcgcgcct cctggctgat gcccggctga cccatgggcc agcgcccgcc
     8281 cggcctttcg acaaccgctt catccaagcc atctacaagg ggacgcaatt gctatggcca
     8341 gcaccatgaa ggcctgggtg cagacaggtt tcggcggacc cgaaacgcgc gtgttgcaag
     8401 aggtgcccgt gccccggccg ggcgctggcg aagtgctggt gaaggtggcc ttttgcgcgc
     8461 tgaaccggct ggatatcctg cagcgccggg cgcggctgat cccggtgatg gcgccgcccc
     8521 atatcgccgg catggatttt gccggcgagg tggtggccgc ggccgggcct gaaggggcag
     8581 cgctggtggg ccggcaggtg gtggtcgacc cggtcgtcac ctgcggcgtg tgcgacaggt
     8641 gccgggcggg gctggcggtc tattgccggc agtttcgcac ctgcggctcc agccgggcgg
     8701 gcgggctggc ggaatatgtg gcggtgccgg cggccaattg catcgtggtg gatggggcgg
     8761 cggcccggct ggcggagctg gcctgcgtgc cggtggcctc ggtcacggcc tggcacgggc
     8821 tgctgggggc gggcggcctg cagccgggcg agacggtggt ggtgcccggc gccggatcgg
     8881 ggctgggcat tgccggcatc cagatcgccc gcgcgcgcgg ctgccgggtg atcaccaccg
     8941 ttgccggccc tgccaagctg gcgcaggccg cggcgctggg cgccgatctg gtgatcgacc
     9001 ggctggccga ggattgggtg agcgcggtgc agggcttcac cgatggcgct ggcgccgatc
     9061 tggtgtggga tcatatcggc gggccgtttt tgcagcaggc gatcgatgcc tgccgggtga
     9121 acgggcgcat tgtcatgtcg ggcaccacgg cgggcaacca gtccaccatc accaacacca
     9181 gcctgttcca ctggggcaag gcgatcatcg gccatggcgg ctacacgccg gccgaaatgc
     9241 gcgctacggt ggccgcttat gccagtggcg ggatgcgcgt ggtgatcgac agcgaatggg
     9301 ccttcgccga cctgcccgcg gccgagcagc ggctggaatc gggcgatttc ttcggcaagg
     9361 tgctggtgtg tctctaggcg gcgtgccggc gatcgccgta tcggggctgg ccaagagcta
     9421 tcggcgcgag ggcgtgccgg tgccggcgct ggcgcccacc gatctggtgg tggaggaggg
     9481 gcggtttgtc accctgttcg ggccgtcggg ctgcggcaaa tccaccctgc tgcggctggt
     9541 ggcggggctg gaagtgcccg atgccggcga ggtgcgcctg tttggccgca gccctgccga
     9601 aaccgcccgc gccaaggcgc tgagctgggc gccgcaatcg cccgcgctgc tgccctggct
     9661 gacgatccgc cagaacctgg ccctgcccca gcgcctcaac cgccgcgccg atcgccaccc
     9721 ggatccggcc cgccagcccc gcccggtgga agaggtgctg gaggaagtgg gcctggccgc
     9781 ctttgccgat gcccgcccgg cgcagctttc gggcggcatg cgccagcggg cggcgctggc
     9841 ccgcgccttt gtggtgggcg cgccgctaat gttca
//
LOCUS       DASRYE010000113        21119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_6397,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000113 DASRYE010000000
VERSION     DASRYE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 21119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21119
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6397"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFF98_06245"
     CDS             <1..253
                     /locus_tag="VFF98_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HZU63264.1"
                     /translation="AARPDPRALALLQAIATAQAEAIKQSRWVGGQFAEEARAMHYGE
                     KPAEMVHGQATREEAEALLEEGVEIAPLLVPVAPPGQVN"
     gene            342..418
                     /locus_tag="VFF98_06250"
     tRNA            342..418
                     /locus_tag="VFF98_06250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:376..378,aa:Arg,seq:cct)
     gene            complement(952..1179)
                     /locus_tag="VFF98_06255"
     CDS             complement(952..1179)
                     /locus_tag="VFF98_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63265.1"
                     /translation="MAINDVQSLQIDEYSEENIISYYKISARTVPSREILEHLFIPPH
                     PAAAKEYRDAQTNPEWQGRDGAYGSGRAALD"
     gene            complement(1334..1504)
                     /locus_tag="VFF98_06260"
                     /pseudo
     CDS             complement(1334..1504)
                     /locus_tag="VFF98_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1749..4211
                     /locus_tag="VFF98_06265"
     CDS             1749..4211
                     /locus_tag="VFF98_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63266.1"
                     /translation="MSVRSFLDNRRFASFRVDLLCGTLIAGGLSLSTPVLAQTAGAAS
                     AGEPVQEVVVTGSRRSDTTALTSLTPVDVVSSDQLRQSGGANLSKLLQTDVPSFHFTQ
                     DNPSTSVQYAVKGASLHGLGPDETLVLVNGIRQHLNSQLNTGYLGYGRGTQVVDIDVI
                     PADSIGRVEVLEDGASAQYGSDAIAGVVNIILKKEDRLTVSSQVGGYTALGQGLQDKS
                     SISWGKTFSNGGFLHATGEFMVSNPIDIAINDTRQYYFTGDSRESTVNRHWFYGSGSY
                     RKFGTMLNGEMPISDALTVFGYANGGYRQNYGFANFRRPNDDGNVRAIYPDGFQPIQR
                     VRSTDYDAALGLRHEGGALGTVSLATSIGSSRASIYTYNTVNASLGTASGTQGSSGIT
                     YNRDLNVTLDQVKSIDVGLGGPLTIASGAAYRHERYQIVAGDAFTWVNGGALILDGPN
                     KGKQAALGSQGASGFQPADAGVWTRDVVGVYADVSVKPTRRLDVDLAGRADHYSDFGW
                     TTNGKVSARFAVTPWLALRGSASTGYRAPSISQLYYTRTSGIFINNIQYLQRILPANS
                     AAAMALGATPLKPEESTNLTAGLVLTPSRHTTITADYYHIDINNRIVLTGTLQGTIVS
                     NILASAGFSQLQGAQFFTNGVDTTTQGLDVLARQDMKVAGGKLALSLGFNWTKTSLRR
                     VEATPSQLASASIVLIDRQQLGFITSGSPNTKLIMSGTYTRGNLGFTATGTRYGTYAY
                     INQTTPSLDQTFSPQWVFGVSAKWQVSPAIRLDVGADNVFNSKPDQQIAGARNPTVSL
                     YSSLSPVGAYGQFTYGRLTFTY"
     gene            4246..5214
                     /locus_tag="VFF98_06270"
     CDS             4246..5214
                     /locus_tag="VFF98_06270"
                     /inference="COORDINATES: protein motif:HMM:NF037872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63267.1"
                     /translation="MGRLIDISWPSVGITVVAELADELNPEMCEEFWRCLPFKVLQAH
                     PVVSGESLYAWAPVVSSAPVHVKERIIDCPVGRLRFSQSTGNKFSIQYGKGLEPLYQP
                     VLGMVLPEYLHLLPKVGKEVWESIFWRKDEIFVHVAPHEAGVVTREGSERDDYPTELM
                     RAFIDEANRIQEIEPEDLRAIRLGQVKGTGTFGQYFSAWDFAQNMLRDYINYTMYVML
                     RLADKQSPQIISQTIDEIDPPYTTYLGHSGLVQLEAFAIQMRDAVREAKDAEEVKLIL
                     SAFLRYGNRLVAWAYHYFPWHIGLFYNRPHDGQELPGRFLQAPIEP"
     gene            5257..6624
                     /locus_tag="VFF98_06275"
     CDS             5257..6624
                     /locus_tag="VFF98_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63268.1"
                     /translation="MAEFDLVLHGTIVSAQAVRSDGWIAVKGEKVAARGTGTPPSALH
                     VVDAGDGWIMPGVIDGQVHSGSQAGQEGLGWASRAAAAGGVTVMVDMPYDDPEPVASA
                     PQLAAKIAEVERDCHVDVALFATVNRLHGMDVIPDLIAGGACAFKFSTFEASPNRFPR
                     VEEDDLYEAFRRIAPSGLACGVHNQMQDITRRNIAQLQEAGETGYDAFYRAHPPLVEN
                     LADVLIYEIGHETGARAHVVHASTDRSFEIFKMFEAAGSPVSIETCVQYLMLNQEDDA
                     ERLGAKIKHYPPIRPRADVAALWRHIARGDCTFVSSDHVSWGLERKGHPDIFRNSSGG
                     PGLETLLPAFWTGCRQHNLPLTTVVQQLCTGPARHFLLDDRKGSLEVGMDADIVVLAR
                     DGTPFDPASSLSAVTWSSFEGMEFDVRVVATWCRGTLVYDDGAIRNQAGFGRFLRPRK
                     TGAAA"
     gene            6621..7874
                     /locus_tag="VFF98_06280"
     CDS             6621..7874
                     /locus_tag="VFF98_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZU63269.1"
                     /translation="MMPEPDINRAWARIEALAAMTRPDMPWTRRAFSPLFDQSRSWLR
                     AEFEKAGLETHVDAAGNLIGTRPGKVAGARPLITGSHCDTVVGGGRFDGILGVLAGIE
                     VAATLHDHGVELDHPFQVIDFLSEEPSDYGISCVGSRGFAGTLTAPMLASTNEEGETL
                     AQAMRRIGADPDALAVPLNGPGSVAAFVELHIEQGPVLENTGIPIGIVTGIVGIRRQR
                     IVVNGQADHAGTTPMDIRHDALAGAARIVTAVNELASRMSGAPHYVVATIGRILMTPN
                     VPNAVPAQVEMVLEMRSDAEEVLAGFSDLALGSVGEALADLRVSVEVSELSRSQPVAC
                     TPAIADVIEAAAGKLGYPTMRLPSGAGHDAAYVAASGLMGMLFVPCLGGRSHCPEEWI
                     EPQQMLDGIRVLQQTLLMLDDTLEV"
     gene            7864..9480
                     /locus_tag="VFF98_06285"
     CDS             7864..9480
                     /locus_tag="VFF98_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZU63270.1"
                     /translation="MKSRAFFLILGTWLISLAVAAPASAGPLPLVADRALTFCADLQN
                     PPTGSLKADGHTPEGVGVDLMNAMAGKLGLGVSILNYRASGVFAALDTGKCDAIMASL
                     SRSPERMKRYDFVDYFRISSGFLVAKGHAGRLKSHNDLAGKRIAVLLGSRNEAWLHEE
                     DDTFAAQGLPRMTIVNLGTNVAAFQDLALGRVDALLSDSIIINYYLSHNAGRFEIAPL
                     PARAGTTLAIAVPKAHPEVAHALRGALDALVEEKTVDRIAHHWGIENGVVPCSTQAPC
                     SREPVGPASVSHVPPVHFDLGFFLHFVLHPPPVILSGILVTLGVAMAAQILGTVLGLG
                     LALLALGRSKAMRVATDLYIWAFRSVPVLVQLVIVYFGLPYLVGFDLFPASMGFGVFA
                     LPGALVAGTLTFGVHEAAMMSEIIRVAIQAIDSGQTEVARSLGMWPGLTLRRIILPQA
                     ARIVLPPLGNQFSNMIKTTSLLSVIGVGELFRVAEDVQAATLMTFEVYLGISVYYLAL
                     IAVTTLMQRWIERRLAPRPRRAPMAQEITQ"
     gene            9477..10220
                     /locus_tag="VFF98_06290"
     CDS             9477..10220
                     /locus_tag="VFF98_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZU63271.1"
                     /translation="MTGQTPALEFRQVSKAFHFTRVLGGVKLCVSAGEIVALIGPSGS
                     GKTTLMRCAAGLASIDTGAILVDGVPVGESKPGAGRQPSIGMVFQKFHLFPHLTTLEN
                     IIEAPVRVLRVKAADARAEAMVLLERMGLADRADYYPAELSGGQQQRVAIARALAMHP
                     KVLLFDEPTSALDPEMVNEVAGVIAEVAATGIAVLLVTHEMAFVRRVAHSVAVMDGGS
                     IVESGATEEVFAAPRHDRTRRFLTAIGLT"
     gene            complement(10213..10842)
                     /locus_tag="VFF98_06295"
     CDS             complement(10213..10842)
                     /locus_tag="VFF98_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZU63272.1"
                     /translation="MSETIHSKNRTNTAGQEPRRIIVGISGATGIVLGIRLLETLRAL
                     DVETHLVVSNPGHATRAYETDLSRQELLALADYNYGIGDFGAAISSGSFRTSGMIIAP
                     CSVRSLAEIATGVTTGLLTRAADVVLKERRKLVLMVREAPLHIGHIRNMAAVTEAGGI
                     IMPPVPAFYQRPASIGDMVDHMVARTLDLFDISVPGFPRWGEEADEPFR"
     gene            10879..11940
                     /locus_tag="VFF98_06300"
     CDS             10879..11940
                     /locus_tag="VFF98_06300"
                     /inference="COORDINATES: protein motif:HMM:NF037872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63273.1"
                     /translation="MAIGRAYKRNGLQDILAGCTAQDKQKSGVIKLIDQNQPETSQSP
                     AGLLKKIDLECEKVIKDEPGELRAIRTGSLENQAGSYGQYFSTMDIAAGMLRDYAGYT
                     MYPLVRMARDPQVPVEHLRRMIEEFDPPYTNYLGYSGLTTLSRFATAVRSIASEASKD
                     ELAELFGALNRYANRVNAWCHHYFPWGLGDQFRYEGDVEEVPAVPASETLPTAAQADI
                     LLRWEPMGIEVRAKLAHDSNPELCADFQAALPFTALQDHAVVTGKSMYAWAPMMSTAP
                     VRVKEQINLAPLGRLRFSQGTGQKLVLQYGPTTETLLAPVLGQVVGEDIPLLDAVGDA
                     VWASTFHDKSLIWITVRAL"
     gene            11969..13618
                     /locus_tag="VFF98_06305"
     CDS             11969..13618
                     /locus_tag="VFF98_06305"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZU63274.1"
                     /translation="MMSTNEIMKELVLDGQTLTPDDVVRAARGDGTGAWPKVSLGEDA
                     RRITEEVRAYVEKNWLTDEAPPIYGINTGLGRLRDVRISAADFESYQRHIIESHSAGI
                     GEPITEEQARATMVLRVNALAKGCSGLRVACLDRLLAMLNAGIHPVIPSQGSVGASGD
                     LAPLAHLVTVMVGHPKAEAFYKGQRMAAADAFAAAGLPTDFDLKPKDVLALINGSTVA
                     LGISILALADAKLLAKNADIALALSVEAMRGELNAFDERIHQVRNCPMQVLVSGNVLK
                     VVSESQWTTEEARQVKLPDEVRPNDIYVPRVQDPYSLRCAPQVHGAARDALLYAETLL
                     GREANAATDNPLIFPDGKGDYVVLSGGNFHGEPIGYAADLIALAVAEIGAISERRSYR
                     LTDPTMSYGLPLNLVGGMLGLNTGFSLVHCSAAALASENKVLCFPSVADSLPTKANQE
                     DHVSMCTFSARKAVMVVKNTSAILAVEFLCAAQGIDLASKTLGERKIGTGSAAAKGRL
                     RNDIDATGEDHYQSSDMYVAQELVRSGALVAAVETAIGAIG"
     gene            13615..15009
                     /locus_tag="VFF98_06310"
     CDS             13615..15009
                     /locus_tag="VFF98_06310"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZU63275.1"
                     /translation="MSHPFPPLPGKNDTQSIRPFLRTLDAGGQLLVARESVDPVHELA
                     ARLSVADGEAAVIFADVAGSSLPVVGNLLSSRERIGQAIGVEATGLTDALLDAIARPV
                     APVQADTAPVQAVVERAAPLAGLPVPTFFAREGRPYITAGVIVARDPRTGSGNASFAR
                     IGILDEHRGMIGIAPNHHLAQFARAAAALGQTLDIAVVLGAHPAIQLAACLYLGLGED
                     EMECAGRFLGEPVRTVRAKTVDLDVPAEAEIVLEGRIDPTDMIDEGLISEYHGMYENY
                     GAGLGVDFTCMTRREDALFQAILPGFHKEHIYLGAVPIAASLRAAIGRVIPNVGDVAV
                     TEAGGGRTDIVIQIDQPKPGQARRAMFAAWGSVSIVKRITIVDADIDPWDVQQVEWAR
                     LSRMRNERDIVIVPGVGADRSEPMEADGMVAKMGFDATMKVGDRGPGMERALPPAVDM
                     ERARAWMRALRHTV"
     gene            15121..17040
                     /locus_tag="VFF98_06315"
     CDS             15121..17040
                     /locus_tag="VFF98_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63276.1"
                     /translation="MHLKRLAYFVVASQQQGGLAAAAAELDITQSTLSAALKVLAEEV
                     GTPLFEHVHRRMHPSRAGLWLFRSALPILHAEEFARSWISARGPDTRVTHLTVDVKLS
                     FALGRVSKAVGCGIQAFSHTHPDIFVEPRFADMRDDDELAAAMPAITAYRHVSLVIDA
                     VASTVCDLEPGIEQMELQRDPWVIVRKHGVQDYEGGDEPFIVPALGEALLRQLGAISE
                     SRAGARVHLADEPAGALPRMLHEQPSASFLMPRSMAADRLGLQNISIQSMEPPVDSLV
                     VARFRKGDNVARSLALEIRKQLEAPERNLIFRPVISLRQMRYHRALYEMGSVTAAARR
                     LLVAQPALTDQLRKLEQTLGAALFIRSRRGLDPTASAVRLRQVSSFVQDRVRRIDIES
                     TKFFGGEASTLRLGVIPSAGPGSLLLQGLAQAIEQWQACYPTMRLQLFEAQSDPLQAM
                     VKAGTVSFAVVERSIPDVARVRLTKAEPLAVVFDPGACVPAKTELSFDDIAHLPLILP
                     TRQSGLRQIIEDAALRAGVRFSPKFEVNSMPLLLQMLRGRALYTIIPVSAARRDINEK
                     ILDFLPISDRNVEREFFAIHSSERNMNASERDFIKILQMELRSEEMKSSDGIKPIRQD
                     IRMHAIKPANQAAWR"
     gene            complement(17284..18189)
                     /locus_tag="VFF98_06320"
     CDS             complement(17284..18189)
                     /locus_tag="VFF98_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63277.1"
                     /translation="MDAKLAGGADRARALALFAAVAQAGSFSAAGRLLGLSPSAVSRA
                     VDRIEARLGVRLLLRSTRALALTAEGRSYLQAARRILADLDDAEQQIADQAAPRGRLR
                     ISAALSHGRLCVVPLLGEFAALYPHILTDIALTDTLVDVAAGAADVAIRFGPLADSTL
                     TARRLGETRRVIVAAPAYLARHGTPAAPEDLHRLNCLNFNFRRAEPVWPFRRDGQDFA
                     LAFRGNIEANNGETLGQLAAAGVGLARVGQFSVEAAIASGALVPVLEAFNPGDVETIH
                     AVFVGGASTPARVRVFVDFLAARLR"
     gene            18307..19482
                     /locus_tag="VFF98_06325"
     CDS             18307..19482
                     /locus_tag="VFF98_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63278.1"
                     /translation="MRLNPGLLALSVGAFGIGVTEFAPMGLLPVIAGDLGVSIPTAGL
                     LISAYALGVVLGAPLMTLTTGRVPRRALLIGLAGIFTAGNLLAALSGSYAMLLVARVI
                     TSFNHGAFFGVGSIVAAGLVPPQRQAGAVAAMFMGLTIANVVGVPLATWAGVHMGWRA
                     SFWGIAALGGLTMAALRLTLPDQPAPEGGNALAELRVLARGRVLGALALTVVGSSAMF
                     TVFTYITPILRDATHASLGFITAMLVTYGLGLTVGNWLGGRYADRSVDRTLIVTLAGL
                     TAVLVAFALLMGHAAPVAVLVFVWGVASFALVPPLQVRVMTAAADAPNLASSVNIGAF
                     NLGNAFGAALGGGVIAAGLGYPAVALAGAATSAAGLLAIVLGGRGQGAALAPCAEAG"
     gene            19522..20187
                     /locus_tag="VFF98_06330"
     CDS             19522..20187
                     /locus_tag="VFF98_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZU63279.1"
                     /translation="MSLPLAEREAVGPAYTLEAICAAQAKSWEALGQIAAQVRPGMRE
                     AEAVAMGEQVLAAHGMELAWHPLMVRFGANTLKIFSDRTPGDAVLGEDDIFFIDMGPV
                     FLGHEGDAGATFTTGTDPEMAACARDVKVLFDKVRAIWAGGAVAGRALYEVAEAEAAA
                     LGWVLNLDIKGHRVGDYPHSAKTGGQLGTLEGAPSAGLWILEMQIRHPTRPFGAFYED
                     LLA"
     gene            20199..>21119
                     /locus_tag="VFF98_06335"
     CDS             20199..>21119
                     /locus_tag="VFF98_06335"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HZU63280.1"
                     /translation="MADLAGIIAQIAAEMADAPDRGQVADYIPALAKVSPAHFGMAVV
                     LADGREYAAGEADLPFSIQSISKVFSLTMALGAVGDALWSRVGREPSGSAFNSIVQLE
                     SEHGIPRNPFINAGAIVVCDILLGRYEPREAIGELLRFVRALAGNEGAGDDSIVIDEV
                     VARGEAETGFRNMALANYMRAFGNIRHPVEKVLGLYFHQCALAMSCRQLARAGRYLMA
                     GGRNPATGFSVVSPQRARRINALMLTCGHYDGSGEFAFRTGLPGKSGVGGGILAIAPG
                     VASIAVWSPGLNDRGNSQLGTLALERLVQKT"
BASE COUNT         3796 a         6190 c         6911 g         4222 t
ORIGIN      
        1 cgctgcccgg cccgatccgc gcgcgctggc gctgctgcag gccatcgcca ccgcccaggc
       61 cgaggcgatc aagcaatcac gctgggtggg cggccagttt gccgaagagg ctcgcgccat
      121 gcattatggc gaaaagccgg ccgagatggt gcacggccag gccacccgcg aggaagccga
      181 ggcgctgctg gaggaaggcg tggagattgc cccgctgctg gtgccggtgg cgccgcccgg
      241 ccaggtgaac tgagcggcgc ggcgatctgc ggcatggccg gccagcccct tgcgcgggat
      301 tgcttttgtg gcaacccttg cctatgagca gcgctctggc cgcgcccgta gctcagcagg
      361 atagagcacc agattcctaa tctgggggcc acaggttcga atcctgtcgg gcgcaccagt
      421 ttcccaacat cgtagtcccg gcggtggtca gcggtacaaa agcgggattg gcgtagggat
      481 ttcggcctaa gatagggcga cccgtcgtgc ccgccgggat cgccgaatgc cgactccttc
      541 caagagccga catctgctcg ttcgcacgct tggaaacgtt gcgttgcacg ggcaaggttg
      601 gccgttagac gattgacgcc tctccgcgcg cgaccgaagc gacctgcact ttgttcaggc
      661 gtgagatgac gtggaaaatt gggctgttga tcgtctgggc ttggtcgact gctgccggtt
      721 ccagccccgc ccgacctgtc gccctgactg ccgcgtgcct acaaatacga cattcagagc
      781 gtctagctgc gtcttcggag ccggtcgtag gttcaggtga tcaaaatcgg caacgaatgg
      841 cccgtctcag ggctgctgcg atcacgctag tcggcgattg agtacgggga atggaccttg
      901 gaactctcga tgttcgcgcc catcacagcg gtctcagcgc catagggcgg gctaatcaag
      961 agccgcacgg ccgctcccgt aagcaccatc tctaccttgc cattcaggat ttgtctgggc
     1021 gtcgcgatac tctttcgcgg ctgcaggatg cggcggtatg aacaagtgct ccaaaatttc
     1081 gcgggatggc actgttctgg cagatatttt gtaataactg atgatatttt cttcgctgta
     1141 ttcatctatc tgaagggatt gaacgtcgtt gatcgccact cttttttata tgttaaattg
     1201 atatatggcg agaacggaca gactgtcctc cagagatatg cttgttagca tgtcgtcgtg
     1261 aaaggggggt aagacatcat gtttaggtaa caagaaagaa taatgtctgc tccataaact
     1321 ggcattccga gcggatcgag ggcctgccga tacgggaata cgggccgccg aaatccgctg
     1381 acaacacgtc cagcgcttca tcaacaatgg cccggatcgc gcggagggga tggttggccg
     1441 gtacccgggc ctcacagctc acgtaagaaa acagcccctc gctccgctcg tcctcacccc
     1501 gtatcccgat cccttcagcc gcacgctacg ccatcctgga atcagcaatg gcgcccgcct
     1561 ggtgcgggaa ctcgacacct tggtgcgccg gtccgttcgg tggcgcttat ggccaatttg
     1621 gcttgatgct atgatgctgg aaaactgcac ccggctagct ttccttcgag cccagtgacg
     1681 tgccgactat gtagggatag cggcgcataa tcactgagag cccgaagggt caaaaagggg
     1741 gcagtccaat gtcagtgcgt tcattcctcg acaatcgccg gtttgcttcc ttccgcgtcg
     1801 atcttctgtg cggaacgctg atcgccgggg ggctgtccct ctcaacgcct gtgctggcac
     1861 agactgctgg cgcggcaagt gcgggcgaac ctgtgcagga agttgtggtg acaggttcgc
     1921 gccgcagcga cacgactgcg ctgaccagcc tgacgccggt cgatgtggtt tcttctgacc
     1981 agcttcggca gagcggcggt gccaatctga gcaagctgtt gcagaccgac gtaccttcgt
     2041 tccacttcac gcaggataat ccttcgacct cggtgcaata tgccgtcaag ggtgcgtcgc
     2101 tgcacggcct tggtcctgat gagacgctgg tgctggtgaa cggcatccgc cagcatctca
     2161 attcccagtt gaacaccggc tatctgggct atggccgtgg tacgcaggtg gtggatatcg
     2221 atgtcattcc agccgattcc atcggccggg tcgaagtttt ggaagatggc gcatcggcgc
     2281 aatacggttc tgatgcgatc gccggggtgg tcaacatcat cctgaaaaaa gaagacaggc
     2341 tgactgtcag ctctcaggtt ggtggctata cggcattggg ccaggggctg caggacaaga
     2401 gttcgatttc ctggggcaag acattttcca acggcggctt cctgcatgcc actggcgagt
     2461 tcatggtgtc caaccccatc gacatcgcga tcaacgatac ccggcaatat tacttcacgg
     2521 gcgattcacg ggaatcgacc gtgaaccgcc actggtttta tggcagcggc agttatcgga
     2581 agttcggcac gatgctgaac ggagagatgc cgatcagcga tgccctgacc gtatttggct
     2641 atgccaatgg tggctatcgc cagaattatg gttttgcgaa cttccgtcgc cccaatgacg
     2701 atggcaacgt ccgcgcgatc taccccgacg gttttcagcc gatccagcgc gtgcgttcca
     2761 ccgattatga cgcggcgctt gggcttcggc acgaaggcgg cgcccttgga acggtcagcc
     2821 ttgcgacatc tatcggttca agccgtgcat cgatctacac ctacaatacc gtcaatgcca
     2881 gccttggcac cgccagcggc acgcagggtt catctggcat cacctataac cgtgatctga
     2941 acgtcacgct ggatcaggtg aaatcgatcg atgtcgggct tggcggcccg ctgacgatcg
     3001 cttccggggc ggcctaccgg catgaacggt atcagatcgt ggcaggtgat gccttcacat
     3061 gggtcaatgg cggggcgttg atccttgatg ggccgaacaa gggcaagcag gcggctctgg
     3121 ggtcgcaggg ggcgtcgggc ttccaaccgg cggatgcggg cgtctggacg cgtgacgtcg
     3181 tgggcgttta tgccgatgtg agcgtcaaac cgaccaggcg actggacgtc gatctggcgg
     3241 gccgggccga ccattattcc gactttggct ggacgaccaa tggcaaggta tcggcccgct
     3301 tcgccgtgac gccgtggctg gccttacgcg gttcggccag caccggctat cgcgctccat
     3361 cgatttcaca gctctactac acccggacca gcggaatttt catcaacaac attcagtatt
     3421 tgcaacgtat tctgccggcc aacagtgccg cggccatggc gctgggcgca acaccgctga
     3481 agccggagga atcgacgaac ctgaccgctg gcctggtgtt gacgccatca aggcacacca
     3541 ccatcacggc ggattattac catattgaca tcaacaaccg tatcgtcctg accggaacgc
     3601 tgcagggtac gattgtgtcg aacattttgg cctcggcagg cttttcccag ttgcagggcg
     3661 cacagttctt cacaaacggg gtggatacca cgacccaggg gctggatgtt cttgcccggc
     3721 aggacatgaa ggtcgccggg ggcaagctgg cgctgtcact gggctttaac tggacgaaaa
     3781 ccagcctgcg ccgggtggaa gcaacgcctt cgcaactggc cagcgccagc atcgtgctga
     3841 tcgatcgcca gcaactgggt ttcatcacca gcggttcgcc caataccaag ctgatcatgt
     3901 cgggcaccta cacccgcggc aatctgggct tcacggcgac aggaacgcgc tatggaacct
     3961 atgcctatat caatcagacc acaccttcgc tggaccagac cttctcaccg caatgggtgt
     4021 ttggggtcag cgcgaaatgg caggtttcgc ctgccatccg gctggatgtg ggggcggaca
     4081 atgtcttcaa cagcaagccg gaccagcaaa tcgcgggcgc ccgcaaccca acggtttcgc
     4141 tttattccag cctgtcacca gtgggggcct acggccagtt cacctacggc cgcctgacct
     4201 tcacctattg atgaactcca tataaattca aggattacag ataaaatggg acgccttatc
     4261 gatatttcgt ggcccagtgt cggcatcacc gtcgtggccg aacttgccga cgagctgaac
     4321 ccggaaatgt gcgaggaatt ctggcgctgc ctgcctttca aggtgcttca ggcgcatccg
     4381 gtcgtctcgg gtgaatcgct gtatgcctgg gcgccggtcg tcagcagcgc gccggtgcat
     4441 gtgaaggaac gcatcatcga ctgccccgtc ggccgcttgc ggttttcgca atccaccggc
     4501 aacaaattct ctatccagta tggcaagggg ctggagccgc tctatcagcc agtgctgggc
     4561 atggtgttgc cggaatatct gcacctgctg cccaaggtcg gcaaggaagt gtgggaaagc
     4621 atcttctggc gcaaggacga aatcttcgtc catgtcgcgc cgcacgaagc tggcgtcgtc
     4681 acccgtgaag gcagcgaacg cgacgattat cccaccgaac tgatgcgggc cttcatcgat
     4741 gaagccaatc gcattcagga gatcgagccc gaagatctgc gggccatccg cttggggcag
     4801 gtgaagggca cgggcacgtt cggccaatat ttctctgcct gggatttcgc ccagaacatg
     4861 ctgcgcgatt acatcaacta cacgatgtat gtgatgctgc gactggccga caagcaaagt
     4921 ccgcagatca tcagccagac gatcgacgaa atcgatccac cctacacaac ctatcttggc
     4981 cattccgggc tggtgcaact ggaagcattc gcgatccaga tgcgtgatgc ggtgcgcgaa
     5041 gcgaaagatg ccgaggaggt caagctgatc ctgagcgcct tcctgcgcta tggcaaccgg
     5101 cttgtcgcct gggcctacca ctatttccct tggcacatcg gcctgttcta caaccgcccc
     5161 catgacgggc aggaattgcc cggccgtttc ctgcaggcgc cgatcgagcc ctgaccgtgc
     5221 gcggcaatct ttttgccgac aatggagttc ccatcaatgg ctgaatttga cctcgttcta
     5281 catggaacga tcgtgtctgc ccaggctgtg cggtccgatg gctggatcgc ggtcaagggg
     5341 gagaaggttg ccgcgcgtgg cacgggcacg cctccctccg cactgcatgt cgtggatgcg
     5401 ggcgatggct ggatcatgcc cggagtcatc gatgggcaag tccattcagg cagccaggcc
     5461 gggcaggaag ggctgggatg ggcttctcgc gctgcggccg ccggcggcgt tacggtgatg
     5521 gtcgacatgc cctatgacga tcccgagccg gtcgcctctg ccccgcaact ggccgccaag
     5581 attgccgagg tggaacggga ctgccacgtt gatgtcgccc tgttcgccac cgtcaatcgc
     5641 ctgcacggga tggatgtgat ccctgatctg atcgccgggg gggcctgcgc attcaaattt
     5701 tccacattcg aagcatcgcc caatcgcttc ccccgcgtgg aggaggatga tctgtacgaa
     5761 gccttccgcc gcatcgcccc atccgggctg gcctgcgggg tgcacaacca gatgcaggat
     5821 atcacgcggc gcaacatcgc ccagcttcag gaagcggggg agactggtta cgatgccttt
     5881 taccgggccc atccgccgct ggttgaaaac ctggccgatg tgctgatcta tgaaatcggc
     5941 cacgaaaccg gtgcccgggc gcatgtcgtc catgcctcta ccgaccgctc gtttgaaatt
     6001 ttcaagatgt tcgaggcggc gggcagccct gtcagcatag aaacctgcgt gcaatatctg
     6061 atgttgaatc aggaagatga tgcggaacgg ctgggcgcga agatcaagca ttatccgccc
     6121 atccgccccc gcgccgatgt agcggccctg tggcgccaca ttgcgcgcgg tgactgcacc
     6181 tttgtgtcat cagatcatgt gagttggggg ctggagcgca agggccatcc cgacattttc
     6241 cgcaacagtt cgggcgggcc gggtctggaa accctgttgc ccgcgttctg gaccgggtgc
     6301 cgtcagcaca atttgccgtt gaccaccgtg gtgcagcagc tttgcaccgg cccggcccgc
     6361 catttcctgc tcgatgaccg caagggttcg ctggaagtcg ggatggatgc cgatatcgtg
     6421 gtactggcaa gggacggtac accctttgac cctgccagca gcctgtccgc tgtaacatgg
     6481 agttcgttcg aaggtatgga gttcgatgtc cgcgtggtgg ctacatggtg ccgtggcaca
     6541 ttggtctatg atgacggtgc catccgtaat caggcgggtt ttggccgctt tctgcgccct
     6601 cgcaagacag gtgcggcggc atgatgcctg aaccggatat caaccgcgcc tgggccagaa
     6661 tagaggctct ggcagccatg acccggcccg acatgccgtg gacgcgccgt gcattttccc
     6721 ccctgttcga tcagagccgc agctggctgc gcgccgaatt cgaaaaggcg gggctggaaa
     6781 cccatgtcga tgcggcaggc aatctgatcg gcacgcggcc cggcaaggtg gccggcgcgc
     6841 ggccgctgat aaccggttcg cattgcgaca cggtggttgg cggcggccgg ttcgacggca
     6901 tactgggcgt gctggcaggg atcgaagtgg cggcgacgct gcacgaccat ggcgtggaac
     6961 tggatcaccc gtttcaggtg atcgatttcc tgtcggagga gcccagcgac tacggtattt
     7021 cctgcgtggg cagccggggc ttcgccggaa cgctgaccgc gccgatgttg gcctccacca
     7081 atgaggaagg cgagacgctg gcccaggcca tgcgccggat cggcgccgac cccgatgcgc
     7141 ttgcggtgcc tctcaacggc ccgggcagtg ttgcggcctt tgtggaattg catatcgaac
     7201 aggggccggt ccttgaaaat accggtatcc cgattggcat cgtcacgggc atagtcggca
     7261 tccggcggca gcgcatcgtg gtcaacgggc aggcagatca tgccggcaca acgcctatgg
     7321 acatccgcca tgatgccttg gccggtgccg cgcggattgt aacggcggtg aatgagctgg
     7381 ccagcaggat gagcggagcg ccgcattatg tggttgccac cattggccgc attctgatga
     7441 cgccgaacgt gcccaatgcc gtacccgcgc aagtggaaat ggtgctggaa atgcgcagcg
     7501 atgccgaaga ggttctggcc ggtttttccg atctggccct tggcagcgtg ggtgaagcgc
     7561 tggccgattt gcgtgtttcg gtcgaagtga gcgaactcag ccggtcccag ccggttgcat
     7621 gcacgcctgc aatcgccgat gtgatcgaag ctgcggcagg gaaactggga taccccacga
     7681 tgcgcctgcc cagtggagcc gggcatgatg ccgcttatgt agcggccagc ggccttatgg
     7741 gcatgttgtt cgtaccctgc cttggcgggc gcagccactg ccctgaagaa tggattgagc
     7801 cgcaacaaat gctcgacggc attcgcgtgt tgcagcaaac tctgctgatg ctggacgata
     7861 cccttgaagt ctagagcgtt cttcctcatc cttggcacgt ggttgatctc gcttgcggtg
     7921 gcagccccgg ccagcgcggg gcccctgcct ttggtcgcag acagggcgct gacattttgt
     7981 gccgatctgc aaaatccgcc gaccggatcc ctgaaagccg acggccacac cccagaggga
     8041 gtgggtgtcg atctgatgaa tgccatggcc ggaaagcttg gccttggtgt gtcgatcctg
     8101 aattaccgtg catcgggcgt ttttgcggca ctggatactg gcaagtgtga tgcgatcatg
     8161 gccagtcttt cgcgatcgcc ggaacgcatg aagcggtatg atttcgtcga ttatttccgc
     8221 atttcgtcgg gtttcctggt ggccaagggg catgccgggc gcctgaaatc ccataatgat
     8281 ctggcgggaa aacggattgc cgtcttgctg ggcagccgca atgaggcctg gctgcacgag
     8341 gaggacgaca cattcgccgc gcaaggcctg ccgcgcatga cgatcgtgaa tctgggcacc
     8401 aatgtggcag cctttcagga tctggcgctg ggccgggttg atgcgctgct ttcagacagc
     8461 atcatcatca actactacct gtcgcacaac gccgggcgct ttgagatagc gcccctgccg
     8521 gcacgtgcag gcaccacctt ggccatcgct gtgcccaaag cccatccaga ggttgcccat
     8581 gccctgcggg gcgcgctgga tgctctggtg gaagaaaaga cggtcgacag gatcgcgcac
     8641 cattggggta tcgaaaacgg tgtggtgccc tgcagcacgc aggcaccatg cagcagggag
     8701 ccggtcggtc cggcatccgt cagccatgtg ccgccggtgc atttcgacct tggcttcttt
     8761 ctgcatttcg tgctgcatcc gcccccggtg atcctgagcg gtattctggt gacgctgggc
     8821 gtggcaatgg cggcgcagat tcttggcacc gttctgggtc tgggcctggc cttgctggcg
     8881 cttggccgat cgaaagcgat gcgcgtggcc accgatcttt acatatgggc atttcgcagc
     8941 gtgcctgtgc ttgtccagtt ggtcattgtc tattttggtt tgccttatct ggtcggtttt
     9001 gatctgtttc ctgcatcgat ggggtttggc gtgtttgccc tgcctggcgc cttggtagcc
     9061 ggtacgctga cctttggcgt gcacgaggcc gccatgatga gcgagatcat ccgcgtggcg
     9121 atacaggcca tcgattccgg ccagacagag gtggcgcgat cgctgggcat gtggccaggc
     9181 ctgacgctgc ggcggatcat tctgccacag gcggcacgca tcgtgctgcc accactgggc
     9241 aaccagttca gcaacatgat caagaccaca tcgctgctca gcgtgatcgg tgtgggcgaa
     9301 ttgtttcgcg tggcagagga cgtgcaggca gcgacactga tgacgttcga ggtttatctg
     9361 gggatttcgg tctattacct tgcgctgatc gcggtgacga cactgatgca acgctggatt
     9421 gaacgccgat tggcaccgcg cccgcgccga gcccccatgg cacaggagat tacgcaatga
     9481 cagggcaaac ccccgcgctg gagtttcgcc aggtcagcaa ggccttccat tttacgcggg
     9541 tgttgggggg ggtaaagctg tgcgtttcag cgggcgagat cgttgcgctg atcggacctt
     9601 ccggttcggg aaaaaccacg ctgatgcgct gcgcggccgg gctggcatcc atcgatacag
     9661 gtgcgattct ggtcgatggc gtgccggtgg gcgagagcaa gccgggggca gggcggcagc
     9721 cgtcgatcgg catggtgttc cagaaattcc acctgtttcc tcatctcacc acgctggaaa
     9781 atatcattga ggcaccggtg cgtgtgttgc gggtgaaagc ggccgacgca agggccgaag
     9841 ccatggtgct gctggaacgg atggggctgg ccgacagggc ggattactat ccggccgaac
     9901 tgtccggcgg gcagcaacag cgtgtggcga tcgctcgcgc tttggcgatg caccccaagg
     9961 ttctgctgtt cgatgaaccc accagcgcgc ttgatcccga aatggtgaat gaagtggctg
    10021 gggtgattgc ggaggtggct gccacgggca ttgcggtgct gctggtcacg catgaaatgg
    10081 ccttcgtccg ccgcgtggcg cacagcgttg ccgttatgga tggcggttcg atcgtcgaaa
    10141 gcggggcaac agaagaggtt tttgcagcgc cccggcatga ccgtacgcgc cggttcctga
    10201 cagcgatcgg cctcacctga acggctcatc cgcttcttcg ccccagcggg ggaacccggg
    10261 cacgctgatg tcgaacaggt caagcgtcct tgccaccata tggtcaacca tgtcgccgat
    10321 cgaggccggt ctctgataga aggcaggcac tggtggcatg atgatgccgc ccgcttccgt
    10381 caccgccgcc atgttgcgga tatggccaat gtgcagcggg gcctcgcgca ccatcagcac
    10441 cagcttgcgt cgttctttca gcaccacatc ggcggcgcgg gtcagcaggc ccgtggttac
    10501 gccggtcgcg atttccgcca gggaacggac cgagcagggg gcgatgatca tgccgctggt
    10561 gcgaaacgag ccggaggaaa tcgcggcgcc aaaatcgcct atgccatagt tgtaatcggc
    10621 aagggcgaga agttcctggc gacttaggtc ggtttcgtag gcccgcgtgg cgtggcccgg
    10681 gttggataca accaggtgtg tttccacatc caatgcccgc aaggtttcaa gcagccgaat
    10741 tccgagcacg atgccggtcg cgcctgaaat tcccacgatg atgcggcgcg gttcctgccc
    10801 ggcggtgttc gttcggtttt tgctatggat ggtttcgctc ataagtgact agcgtcagcc
    10861 ctcgccggat gaaagatatt ggccatcggc agggcctaca aacgaaatgg tctgcaggat
    10921 atattggcgg gttgcactgc tcaagataaa caaaaatcag gagtgatcaa gttgattgac
    10981 caaaatcagc cggaaacatc acaatcacct gcaggtcttt tgaagaagat tgatcttgaa
    11041 tgcgaaaagg tcatcaagga tgagcctggt gagcttcgtg caataagaac gggcagcctt
    11101 gaaaatcagg ctggatctta tggtcaatat ttcagcacta tggatatcgc tgctggaatg
    11161 ttgcgcgatt atgccggata caccatgtat ccgttggtgc gcatggcgcg cgatccgcaa
    11221 gtgccggtgg aacatttgcg ccggatgatc gaggaattcg atccgcccta taccaattat
    11281 ctgggataca gcgggctgac gacgctcagc cggtttgcca cggcggtccg ttccatcgca
    11341 tctgaggcga gcaaggatga gctggcggaa ctgttcgggg cgctcaaccg gtatgcaaac
    11401 cgtgtaaatg cctggtgtca ccattacttt ccctgggggc tgggcgatca gttccgctat
    11461 gaaggcgatg tggaagaggt tccggccgtg ccggccagcg aaaccctgcc cactgcggcc
    11521 caggcggaca ttctcctgcg ctgggaacct atgggtatcg aagtgcgtgc gaagctggcg
    11581 catgattcca accccgaact atgcgcggat tttcaggctg ccctgccatt caccgcattg
    11641 caggaccacg cggtcgtaac gggcaaatcc atgtatgcct gggcgccgat gatgagcacg
    11701 gcgccggtgc gggtgaaaga gcagatcaat ctggccccgc ttggccgctt gcgcttttca
    11761 cagggcacgg ggcagaaact ggttctgcaa tacggcccaa ccaccgaaac gttgctggcc
    11821 cccgtgctcg gtcaggtggt gggggaggat attccgctgc tggatgcggt tggtgatgcc
    11881 gtctgggcaa gcacttttca cgataaatcg ctgatctgga ttaccgttcg cgcgctgtga
    11941 tgtgcgtgca aacaacagga aaggggacat gatgtccacc aatgaaatca tgaaggaact
    12001 ggtccttgat ggccagacgc tgacgcccga cgacgtggtg cgtgccgcgc ggggtgatgg
    12061 caccggcgcc tggcccaagg tgtcgctggg tgaagacgcg cggcggatta ccgaagaggt
    12121 gcgcgcctat gtcgaaaaga actggctgac cgacgaagcg ccgccgatct atggcatcaa
    12181 caccgggctt ggccgcctgc gcgatgtgcg gatcagcgct gcggattttg aaagctatca
    12241 acgccacatc atcgaatccc attcagccgg gatcggcgaa cccatcacgg aagaacaggc
    12301 ccgcgcgacc atggtgctgc gcgtcaatgc cctggccaag gggtgttccg ggctgcgcgt
    12361 tgcctgcctt gaccggttgc tggccatgtt gaacgcgggt attcatccgg tgatcccgtc
    12421 gcaaggttct gtgggggcca gtggcgattt ggctccgctg gcgcatctcg ttacggtgat
    12481 ggtcggccac cccaaggcag aggcgttcta caaggggcag cgtatggccg ctgccgatgc
    12541 tttcgccgct gccggtctgc ccaccgattt cgatctgaag cccaaggatg tgctggccct
    12601 gatcaacggt tccaccgtgg cgctgggtat ttcgatcctt gcgctggccg atgccaagtt
    12661 gctggcgaag aacgcggata tcgcgctggc tttgtcggtg gaagcgatgc gcggggaact
    12721 caacgcattt gatgagcgca tccaccaggt gcgtaactgc cccatgcagg tgctggtttc
    12781 cggcaatgtg ctgaaggtgg tgtctgaaag ccagtggacc acggaagagg cccgccaggt
    12841 gaaactgcct gacgaagtgc gccccaatga tatctatgtg ccgcgtgtcc aggatcccta
    12901 ttcactgcgt tgcgccccgc aggtccatgg tgccgcgcgc gacgccttgc tctatgctga
    12961 aacgctgctg gggcgcgagg cgaatgcggc caccgacaac cccctgatct tccccgacgg
    13021 gaagggggat tacgtggtgc tgagtggcgg caatttccat ggtgagccga tcggatatgc
    13081 tgccgatctt atcgcgctgg ccgttgctga gatcggtgcg atttccgaac ggcgttccta
    13141 tcgcctgacc gatccgacga tgagctatgg cctgccactc aatctggtgg gcggtatgct
    13201 ggggctcaac accggctttt cgctggtgca ttgcagcgcg gcagcgctgg cgtcggaaaa
    13261 caaggtgctg tgcttccctt cggtggcgga ttcgctgccc accaaggcca accaggaaga
    13321 ccatgtttcc atgtgcacct tttcggcgcg caaggcggtg atggtggtga agaacacctc
    13381 cgctatcctt gcggtggaat tcctttgcgc ggcgcagggt atcgatctgg cctccaagac
    13441 ccttggcgaa cgcaagatcg gcaccggttc agccgcagcc aaggggcgct tgcgcaatga
    13501 catcgatgcg acgggtgagg accattacca gtcgagcgat atgtatgtgg cccaggaact
    13561 cgttcgctcg ggcgctttgg tggcggccgt ggaaaccgcg atcggggcta tcggatgagc
    13621 catcctttcc cgcccttgcc gggcaagaac gacacccagt cgatccgccc gttcctgcgc
    13681 acgctggatg caggcggcca gcttctggtg gccagggaaa gcgtcgatcc ggttcatgaa
    13741 ctggccgcgc gcctttccgt tgccgatggc gaggcagcgg tgatcttcgc cgatgtggcg
    13801 ggatcatcgc tgcccgtggt gggcaatctg ctaagcagcc gggaacgcat cgggcaggcg
    13861 atcggtgtcg aggcaacagg ccttacagat gccttgctgg atgccatcgc gcggcctgtt
    13921 gcgccggtgc aggccgacac ggcaccggtg caggcggtgg ttgaacgggc agcgcccctg
    13981 gctggccttc cggtgccgac tttcttcgca cgggaaggtc ggccctatat caccgccggg
    14041 gtgatcgtgg cacgcgaccc gcgcactgga agcggcaacg cctctttcgc gcggatcggc
    14101 attctcgacg agcatcgggg catgatcggt atcgccccca atcatcatct ggcccaattc
    14161 gcacgggcgg cggcggctct tggccaaacg ctggatattg cggtggtgtt gggggcgcat
    14221 ccggcgatcc agcttgccgc atgcctctat cttggcctgg gtgaggatga gatggaatgc
    14281 gccggccgtt ttctgggcga gccggtacgg accgtgcgcg ccaaaacggt cgatctggat
    14341 gtgccggcag aggccgagat cgtgctggaa gggcggattg accccaccga catgatcgat
    14401 gaagggctga tttccgaata ccatggcatg tatgaaaatt acggcgccgg tttgggggtg
    14461 gacttcacat gcatgacccg acgggaagat gccttgtttc aggcgatctt gccgggcttt
    14521 cacaaggaac atatctatct tggcgcggtg ccgatcgcgg ccagcctgcg tgcggccatc
    14581 gggcgggtga tccccaatgt cggtgacgtt gcggtgacag aggccggcgg ggggcgcaca
    14641 gacatcgtca tccagatcga tcagcccaag cctggtcagg cgcggcgtgc gatgttcgcg
    14701 gcatggggtt ccgtttcgat cgtcaagcgg atcaccattg tggatgcgga tatcgatccc
    14761 tgggatgtgc agcaggtgga atgggcccgg ttgagccgca tgcgcaacga gcgtgatatc
    14821 gtgattgtgc cgggggttgg tgcggatcga tccgagccga tggaggccga cggcatggtt
    14881 gccaaaatgg gctttgatgc aacgatgaaa gtgggtgatc gcggccctgg catggaaagg
    14941 gccctgccgc cagccgttga catggaacgg gcgcgtgcat ggatgcgtgc attgcggcat
    15001 acggtgtagc agaaccgaca agggtggttg ctcccaccat tggatgtgtc agtattatga
    15061 cggagatggt gctcggatgg gtcgttcgaa cgggaacgcg cactttatcg ggtatcgcac
    15121 atgcatctga aacgcttggc ttatttcgta gtggcatcgc agcagcaggg cggacttgct
    15181 gccgcggcgg ctgagcttga catcacccag tcgactctga gcgctgcgct gaaggttctg
    15241 gccgaggagg ttggcacacc gctgttcgaa catgtgcacc ggcgcatgca tcccagcagg
    15301 gcggggttgt ggctgttccg ttctgctttg ccgattctgc atgcggaaga attcgcgcgt
    15361 tcatggatta gtgccagggg gcctgatacc cgggtgacgc atcttaccgt cgatgtgaag
    15421 ctaagcttcg ccttgggccg cgtgtcaaag gcagtgggat gcggaattca ggccttttcc
    15481 catacccatc cggatatttt cgtggagcca cggttcgcgg atatgcgcga tgacgatgag
    15541 ctggccgctg cgatgccggc gattaccgca tatcggcatg tgtcactggt gatcgatgcc
    15601 gtggcgagca cggtttgcga tctggagccg ggcatagagc agatggaatt gcagcgcgat
    15661 ccctgggtga tcgtgcgcaa acatggggtt caggactacg aaggtggcga cgagcccttt
    15721 atcgttcccg cgctgggcga agccttgctg cgccaactgg gcgccatttc cgaaagcagg
    15781 gcgggcgccc gcgtccatct ggcggacgaa cctgcggggg ccttgccgcg catgctgcat
    15841 gaacagccct ctgccagttt cctgatgccg cgcagcatgg ccgccgacag gctggggttg
    15901 cagaacatct ctatccagtc gatggaaccg ccggtggaca gtctggttgt ggcgcgtttc
    15961 cgcaagggtg ataacgttgc gcgttccctg gcgctggaaa tccgcaaaca gttggaggcg
    16021 ccggaacgca acctgatctt ccgccccgtt atcagcctgc gtcagatgcg ttaccaccgg
    16081 gcactttacg aaatggggag tgtgacggct gctgcgcgtc ggctgcttgt ggctcaaccg
    16141 gcgttgaccg atcaactgcg gaagctggaa cagactttgg gggccgcgct gttcatccga
    16201 tcccgacgcg ggctggatcc gacagcctcg gcagtgcgtc tgcgtcaggt cagttctttc
    16261 gtgcaggatc gcgtgcggcg catcgatatc gaaagtacga aattcttcgg cggagaagcc
    16321 agtacattgc gattgggcgt catcccaagc gcggggcctg gcagcctttt gctgcaaggc
    16381 ctggcgcagg cgatcgagca atggcaggca tgctatccga ccatgcgcct gcagcttttc
    16441 gaagcgcaaa gcgatccgtt gcaggcgatg gtgaaggccg ggacagtcag cttcgccgtg
    16501 gtggaacgca gcattcccga tgtggcacgc gtaaggctga ccaaggcaga accgcttgcg
    16561 gtggttttcg atcccggtgc atgcgtgccg gccaaaacag aattgtcgtt cgatgatatt
    16621 gcccatttac cgctgatcct tcccacccgg caaagtggat tgcgtcagat catcgaggat
    16681 gctgccttaa gggcaggggt gcgtttttct cccaaattcg aagtcaattc catgcctctg
    16741 ctgctgcaaa tgctgcgggg gcgcgccctg tatacgatca ttcccgtcag cgcggcccga
    16801 cgggacatca acgaaaagat actcgatttt ctgccgattt ccgacaggaa cgtcgaaagg
    16861 gaattttttg ccatccattc atccgaacga aatatgaatg cttcagagcg tgatttcata
    16921 aaaatacttc aaatggaact tcgatcggaa gagatgaaat catcggacgg gatcaagcca
    16981 atcaggcagg atattcgcat gcatgcgatc aagcccgcta atcaggcagc ctggcgctga
    17041 gtttccgtcg ttttggcgct cctttgtgtc cgatacctaa gaagcaaggc tcgggcgatg
    17101 ggaaattgcc gccaagggcg cagggcatgc atccagaggc tggcggtaat gggcagtgca
    17161 tggcggtatg ctgcggttct agcgtagcaa aagcggcgct tcatatgcac cggattcgtc
    17221 cggcttgggg caccagtttc ccctgccatt gcggttttgc ccgggctgcg tcgggcccgc
    17281 cttctatctg aggcgcgcgg cgaggaaatc gacgaagacg cgcacccgtg ccggcgtgct
    17341 ggcgccgccc acgaacaccg cgtggatcgt ttccacatcg ccggggttga acgcctccag
    17401 caccggcacc agcgccccgc tggcgatcgc cgcctccacc gaaaactggc ccacgcgcgc
    17461 cagccccacg ccggccgcgg ccagctggcc cagcgtttcg ccattgttcg cctcgatgtt
    17521 gccgcgaaag gcgagggcga aatcctgccc gtcgcggcgg aagggccaga cgggttcggc
    17581 gcggcggaag ttgaagttga ggcagttgag gcggtgcagg tcctcgggcg cggcgggcgt
    17641 gccatggcgg gccagatagg cgggcgctgc cacgatcacc cggcgcgtct cgcccagccg
    17701 gcgcgcggtc agcgtgctgt cggccagcgg gccgaagcgg atcgccacat ccgccgcgcc
    17761 cgccgccaca tcgaccagcg tgtcggtcag cgcgatatcg gtgaggatat gcggatagag
    17821 cgcggcaaat tcgcccagca gcggcaccac gcacagccgc ccgtgcgaca gcgccgcgct
    17881 gatccgcaag cgcccgcgcg gggcggcctg atcggcgatc tgctgctcgg catcgtcgag
    17941 atcagccagg atgcgtcgcg cggcctgcag ataggaacgc ccctcggcgg tgagcgccag
    18001 cgcccgggtg gagcgtagca gcagccgcac gccaagccgc gcctcgatcc ggtcaaccgc
    18061 ccggctgacg gccgaggggc tgaggcccag cagccgcccg gcagcggaaa aactgcccgc
    18121 ctgcgccacc gccgcaaaca gcgccagggc gcgggcgcga tcggcgccgc cagccagctt
    18181 tgcgtccatt gcaaaaatcc ttcgcgtggg cgggtgatat tccaaagcgc ttcccgcccc
    18241 tatctatgcg tctccatcgc agcggcaaag ccccctcttg ccgcccatcc caggatcctt
    18301 cccgccatga gactgaaccc aggcctgctt gccctttccg tcggtgcctt tggcattggc
    18361 gttaccgaat ttgcgccgat gggcctgctg ccggtgatcg ccggcgatct gggcgtgtcg
    18421 atccccacgg ccggcctgct gatcagcgcc tatgcgctgg gcgtggtgct gggcgccccg
    18481 ctgatgacgc tgaccactgg ccgcgtgccg cgccgcgcgc tgttgattgg cctcgctggc
    18541 atcttcacgg cgggcaatct gctggcggcg ctgtcgggca gctatgcgat gctgctggtg
    18601 gcgcgggtga tcacctcgtt caatcatggc gccttctttg gcgtgggctc catcgtggcg
    18661 gcggggctgg tgccgccgca gcggcaggca ggcgccgtgg cggcgatgtt catggggctg
    18721 acgatcgcca atgtggtggg cgtgccgctg gccacatggg cgggcgtgca catgggctgg
    18781 cgggcctcct tctggggcat cgccgcgctc ggcggcctga cgatggccgc cctgcggctg
    18841 accctgcccg atcagccggc gccggaaggc ggcaatgcgc tggccgagct gcgcgtgctg
    18901 gcgcgcgggc gggtgctggg ggcgctggcg ctgacggtgg tgggatcgag cgcgatgttc
    18961 accgtcttca cctacatcac gccgatcctg cgcgatgcca ctcatgcctc gctgggcttc
    19021 atcaccgcca tgctcgtcac ctatgggctg gggctgacgg tgggcaactg gctgggcggg
    19081 cgctatgcgg atcgttcggt ggatcgcacg ctgatcgtca cgctggcggg gttgacggcg
    19141 gtgctggtgg cctttgccct gctgatgggc catgcggcgc cagtggcggt gctggtgttc
    19201 gtgtggggcg tggccagctt tgccctggtg ccgccgctgc aggtgcgggt gatgaccgcc
    19261 gctgccgatg cgcccaacct tgcctcctcg gtcaatatcg gcgcgttcaa cctgggcaat
    19321 gcctttggcg cggcgctggg cggcggggtg atcgcggcgg ggctgggcta tccggcggtg
    19381 gcgctggcgg gcgcggccac atcggcggca gggctgctgg cgatcgtgct gggcggccgc
    19441 ggccagggcg ccgcgcttgc cccttgcgca gaggcgggct aggctgggcg ctcctgcttc
    19501 ttttcaaagg tttgacagcc catgtccctg ccgcttgccg aacgcgaggc cgtgggccct
    19561 gcctacacgc tggaggcgat ctgcgcagcg caggccaaaa gctgggaagc gctggggcaa
    19621 atcgccgcgc aggtgcgccc gggcatgcgc gaggcagagg cagtggccat gggcgagcag
    19681 gtgctggccg cccatggcat ggagctggcc tggcatccgc tgatggtgcg ctttggcgcc
    19741 aacacgctga agatcttttc cgatcgcacg ccgggcgatg ccgtgctggg cgaggatgac
    19801 atcttcttca tcgacatggg cccggtgttt ctgggccatg agggcgatgc cggcgccact
    19861 ttcactaccg gcacggaccc ggaaatggcc gcctgcgcgc gcgacgtgaa ggtgttgttc
    19921 gacaaggtgc gggcgatctg ggcaggcggc gcggtggccg gccgggcgct gtatgaggtg
    19981 gccgaggcag aagcggcggc gctgggctgg gtgctcaacc tcgacatcaa ggggcaccgc
    20041 gtgggcgatt atccgcattc ggccaaaacc ggcggccagc tgggcacgct ggaaggggcg
    20101 ccgtctgccg gcctgtggat cttggagatg cagatccgcc accccacccg gcccttcggc
    20161 gctttctacg aggatctgct ggcgtaaggg gaaagcagat ggccgatctt gccgggatca
    20221 tcgcgcaaat cgccgcggaa atggcggatg cgcccgatcg cgggcaagtg gcggattata
    20281 ttccggcgct ggccaaggtg tcgccggcgc atttcggcat ggccgtggtg ctggccgatg
    20341 gccgcgaata tgccgccggc gaggccgatc tgcccttctc gattcagagc atctccaagg
    20401 tcttcagcct gacgatggcg ctgggcgcgg tgggcgatgc gctgtggagc cgggtggggc
    20461 gcgagccttc gggcagcgcc ttcaactcga tcgtgcagct ggaaagcgag catggcatac
    20521 cgcgcaaccc cttcatcaac gccggcgcta tcgttgtgtg cgatatcctg ctgggccgct
    20581 acgaaccgcg cgaggcgatc ggcgagctgc tgcgcttcgt gcgcgcgctg gccggcaatg
    20641 agggggcggg cgatgacagc atcgtgatcg acgaggtggt ggcccgcggc gaggcggaaa
    20701 ccggctttcg caacatggcg ctggccaatt acatgcgcgc ctttggcaat atccgccatc
    20761 cggtggaaaa ggtgctgggc ctctatttcc accaatgcgc gctggcgatg agctgccggc
    20821 agctggcccg cgcgggccgc tatctgatgg ccggcgggcg caatccggcc accggctttt
    20881 cggtggtttc gccccagcgg gcgcggcgca tcaatgcgct gatgctcact tgcggccatt
    20941 acgatggatc gggcgaattt gccttccgca ccgggctgcc cggcaaatcg ggggtgggcg
    21001 gcggcattct ggcgattgcg cccggggtgg cctcgatcgc ggtatggtcg ccggggctga
    21061 atgacagggg caattcgcag ctgggcacgc tggcgctgga acggctggtg cagaaaacc
//
LOCUS       DASRYE010000114         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_162773,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000114 DASRYE010000000
VERSION     DASRYE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162773"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..889)
                     /gene="nifH"
                     /locus_tag="VFF98_06340"
     CDS             complement(14..889)
                     /gene="nifH"
                     /locus_tag="VFF98_06340"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HZU63281.1"
                     /translation="MSLRQIAFYGKGGIGKSTTSQNTLAALADLGQRILIVGCDPKAD
                     STRLMLHAKAQDTILSLAAEAGSVEDLELEDVMKIGYKDIRCVESGGPEPGVGCAGRG
                     VITSINFLEENGAYDNIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYA
                     ANNISKGILKYANSGGVRLGGLVCNERQTDKELELAESLAKKLGTSLIHFVPRDNIVQ
                     HAELRRMTVIEYAPESNQAEEYRQLAHKIHNNAGKGIIPTPITMDELEDLLMEHGIMK
                     SIDESQVGLTAAQLA"
     gene            1304..1735
                     /locus_tag="VFF98_06345"
     CDS             1304..1735
                     /locus_tag="VFF98_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU63282.1"
                     /translation="MQIGVETEKAVDQRRIFVVDDDEITRAALQFMLHDEIETHELPD
                     LASAYAKASDWLMPDLVILGIGLVKAGGTRIIADIADRFPRARVLLATGREDEPLALA
                     ALAAGAHGALVKPLRIEAVRQKVDTILGRAGGMPLVQLGGL"
     gene            1763..1969
                     /locus_tag="VFF98_06350"
     CDS             1763..1969
                     /locus_tag="VFF98_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZU63283.1"
                     /translation="MKISARNQISGTVTAITPGAVNGQIKVDIGGGNAITSSITEEAI
                     AELGLAVGDAVTVIIKASDVLIGK"
     gene            complement(2022..2429)
                     /locus_tag="VFF98_06355"
     CDS             complement(2022..2429)
                     /locus_tag="VFF98_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZU63284.1"
                     /translation="MLPGIVTAIVHGPINAEVGLLVNDRLVIDALITNSSVEALQLRP
                     GGRAVALIKASFVTLLEDVPGLRLSARNLIGGRVCAVQEGPVESEVVLDIGGGCEMAA
                     IVTTHSLHEMRLEIGSHTLAAFKASHIILAVEG"
BASE COUNT          405 a          819 c          792 g          462 t
ORIGIN      
        1 gggcggccgc ggatcaggcc agttgcgcgg cggtcaggcc cacctggctc tcgtcgatgc
       61 tcttcatgat gccgtgttcc atcagcaggt cctccagctc atccatggtg atgggcgtgg
      121 ggatgatgcc cttgccggca ttgttgtgga tcttgtgcgc cagctgccgg tattcctcgg
      181 cctggttcga ttccggcgca tattcgatca ccgtcatgcg gcgcagttcg gcatgctgca
      241 cgatgttgtc gcgcggcacg aagtggatca ggctggtgcc cagcttcttg gccagcgatt
      301 cagccagttc cagctccttg tcggtctggc gctcgttgca caccagcccg cccaggcgca
      361 caccgcccga attggcgtat ttgagaatgc ccttcgagat gttgttggcc gcatacatgg
      421 ccatcatctc gcccgacatg acgatgtaga tttcctgggc cttgttctcg cggatgggca
      481 tggcaaagcc gccgcacacc acatcgccca gcacgtcata cgagacatag tcgatgttgt
      541 cgtaggcgcc gttctcttcc aggaagttga tcgaggtgat caccccgcgc ccggcgcagc
      601 ccacgcccgg ctctggcccg ccgctttcca cgcagcggat gtccttgtag ccgatcttca
      661 tcacatcctc gagctcgagg tcttccaccg agccggcttc ggcggccagg ctgaggatcg
      721 tgtcctgcgc cttggcatgc agcatcaggc gggtggaatc ggccttggga tcgcagccga
      781 cgatgaggat gcgctggcca agatcagcca gcgcggcgag ggtattttgc gatgtggtgg
      841 acttgccgat gccgcccttg ccgtagaagg cgatctgacg aagtgacata agcctgctcc
      901 tcgttggttg tcacgcgaac ttcccgccag gccttggcca ggcctggcgg cactcccccg
      961 tcgctggtgc ttcttgtggg cggcgggtca gtcggcgcgg gggctcaggg gccctcgtcc
     1021 gctgcacagg cttgttcgca aagcccgtgc caattgcccg gcatcgccat gcagatcccg
     1081 gttttccgcc atttgcaggc cttgtgcgca gcggcaccgg cggtgtgcct ttcctgacat
     1141 tcgcccggcg cctgtcagga aaggcaccct cttgtcgcaa agagcacacg ccggcccggc
     1201 ccatctccgg cggtttcggg caggtttcgg gcataggggc gattggaacg gaacttgctg
     1261 ccctatcccc gccatttccg gccggcacga aaggggtgtt cccatgcaga ttggcgttga
     1321 aaccgaaaag gcggtggacc agcggcgcat cttcgtcgtc gatgacgacg agatcacccg
     1381 cgcggccctg caattcatgc tgcacgacga gatcgagacg catgagctgc ccgaccttgc
     1441 cagcgcctat gccaaggcca gcgactggct gatgcccgat ctggtcatcc tggggatcgg
     1501 ccttgtcaaa gcgggcggca cgcgtatcat cgccgatatt gccgacaggt tcccccgcgc
     1561 gcgggtgctg ctggccaccg gccgggaaga cgagccgctg gccctggccg cgcttgcagc
     1621 cggcgcccat ggcgcgctgg tgaagccgct gcgcatcgag gcggtgcgcc agaaagtgga
     1681 taccattctt ggccgggccg gcggcatgcc gctggtccag ctgggcggac tgtaacaagc
     1741 ttcaaccaca ggagacaggc acatgaagat cagcgcgcgc aaccagattt ccggcaccgt
     1801 caccgccatc acgcccggcg cggtgaacgg gcagatcaag gtggatatcg gtggcggcaa
     1861 tgccatcact tcctcgatca ccgaggaagc catcgccgag ctgggcctgg ccgtgggcga
     1921 tgccgtaacg gtgatcatca aggccagcga tgtgctgatc ggcaagtaag ccgcacgcac
     1981 gccagaaagg ggccggcgcc agcgcccgcc cctttttgct atcagccttc caccgccaga
     2041 atgatgtgcg aggccttgaa ggcagccagc gtgtggctgc cgatttccag ccgcatctcg
     2101 tgcagcgaat gggtggtgac gattgccgcc atctcgcagc cgccgccgat atcgagcacc
     2161 acctcgcttt ccaccggccc ttcctgcacc gcgcagaccc ggccgccgat gaggttgcgc
     2221 gccgacagtc tgagccccgg cacatcctcc agcagggtga caaagctggc cttgatcaac
     2281 gccaccgcgc ggccgccggg gcgcagttgc agcgcctcca cgctgctgtt cgtgatcagc
     2341 gcgtcgatca ccagccggtc gttcaccagc aggcccacct cggcgttgat cggcccgtgc
     2401 acaatcgccg tgacgatgcc gggcagcatg ttgcgcgcgc tgatgcgcag cgggcgcggg
     2461 cgcaacagcg gcagggcg
//
LOCUS       DASRYE010000115        14183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_180331,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000115 DASRYE010000000
VERSION     DASRYE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180331"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2819)
                     /locus_tag="VFF98_06360"
     CDS             complement(<1..2819)
                     /locus_tag="VFF98_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HZU63285.1"
                     /translation="MNRTLFLAGAAALALAPLPALAKPDAGPAAAPLPTPQAVLGHMM
                     GENHYVPSYEDMLRYWRALAARSDRIRLIDIGQTTQGRHQIMAVVSSPENLARLDHYR
                     EISRQLGTAEGIDAAAARQLAGEGRALVWVDGGLHAAEVEAQTALIQQSYDLVAAEDA
                     EARRIRDQVITLLVPDNPDGQQFVADWYMRIADPDKREPDLTSLPRLYHPYIGHDNNR
                     DFYMAEMVETRNMADVLYKSWRPQIIFNQHQTGPSGTVVFLPPFRDPFNYHYEPLVIS
                     SLDEVGAALQSRLLAEGKPGATTRSGAHYDTWYNGNLRTSAYFHNAIGLLVEIIGSPV
                     PERIPFVPERQVPGNDLPAPVAPQMWTMAQSIAYSVSLERAVLAYAAGNREKLLYNRW
                     QMGADAIARGNTDQWTISTDRIAAAHAAAAAAGEAPRPTRTTGSSEIGKAYYDSVLHD
                     PAQRDPRAFIVPVAQHDFPTAARFLTALTRLGVDVRRARAPFQAAGRSWPAGTYIVPA
                     AQAYRAHVLDMFEPQHYPNNFQYPGGPPIPPADASGYTPAFQMGVEFARALDGFAADS
                     VPVAGEIAPDPVPVAGAVAHPAGWLVSHAQNNSFLLVNRLLKAHVAVAEMRASLPVAG
                     GPAAPGAIWVPAGGAAGGIVAEGARSLGIVAEALATAPSGPRIALKPQRVALVDLYGG
                     LHPTGWLRWIFDGYELPYRIVYPRQLDAGHLKRAFDVVILPDAAIPNARTGGAGGMFR
                     GRFENAQPKPADIPAQYRPSLGAISAERTLPALAGFVTQGGVLVAMGSSSAGLARAMD
                     LPVSDPLSEAPSGSGADGRPQPLPRSRFYIPGALLTATADQQDPIAWGLAAQPDLFFD
                     ASPVFRLKPGTPGASVPLAFGAGPLLHSGWAWHAEYLAGTGAVVSLPRGRGRVVLVGP
                     EIALRGQTQGAFKILFNAITLPAAAG"
     gene            complement(2816..4117)
                     /locus_tag="VFF98_06365"
     CDS             complement(2816..4117)
                     /locus_tag="VFF98_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63286.1"
                     /translation="MNASIPRPVPGRAELARALGSAMVGNWFELFDFIIYGYFAAQIG
                     KAMFPAADPLTSVLSSFATYGVGFLMRPVGSMVLGGMGDRLGRKSALVLTMLMMAGST
                     CATGLVPDYARIGLAAPALLLLCRLVQGFAAGGEWGGATTFLVEYAPAGRRGLFGALQ
                     QMSTNVAVVTAVLVALGLNAALSAQAMQAWGWRVPFVLGVLIAPLGFYLRRNVAETPG
                     FVAETAAERPLHPLREAFTTQRRTMLTIGAMVMVWTVAGYTYGTFLVSFAGQVLHLPA
                     RAALTGTLVGALVNIAVIPLAGALSDKVGRKPFMAASAAGFLICSLPLFVLMAQARSP
                     AAVLVATGVAGLFSGLFSGTAPTYLCELLPTRLRYTALSVGYNGAVMLFGGFAPFIGT
                     LLVHATGLAFAPAFYVMGAAALSLVTILLVPAPDPHTAIDR"
     gene            complement(4114..5565)
                     /locus_tag="VFF98_06370"
     CDS             complement(4114..5565)
                     /locus_tag="VFF98_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63287.1"
                     /translation="MSISRKNMAAALPLAAALLLPTPAAAQDHPSVPLTDRRLWEAPG
                     TNTDDTPRRVPERPVAGAQPVTVLRGGLVFDSESLAVRPLTVVIAGKRIARLLPPGAA
                     DWPADAQVIDVAGQFVMPGLIDMHVHITYPDSDTPIDVQASEADGVLRGIRNLRWFIE
                     AGFTSVRDLNGVGDAPYRLADAAAADAIPAPRVFTAGHIITGTGGHATERPVGPTHGD
                     MYAWQRDGADAWRAAVRQTFRKGATVIKLASHFAPDEVQAAVDEAHRLGLKVTCDCET
                     IYTKMAVDAGIDMIEHPLPRTDETIAAMARRHTAAVPTLQVYQNVIDRAGGFWGSTSR
                     RFTMTSDDNFAVFRKMKAAGIVMGVGTDTIGDANRMTPNIYLAELKWFVKGGYTIPQA
                     LQAATLTNARLLDMGDELGRIAPGMLADVLVVAGRPDQDIDALHHVALVVKDGQVMVR
                     GGQVVIMPHVPRPLPGPVSGPAHAAAAAESHAP"
     gene            complement(5562..6326)
                     /locus_tag="VFF98_06375"
     CDS             complement(5562..6326)
                     /locus_tag="VFF98_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU63288.1"
                     /translation="MTDEIKSAARVLDMLEMFSTRCDAMTLSEVARGLSLPKSSALGL
                     LRTLHKRGYLVRVDDNDAYQLNDMIRRDGFARHGRLLRVAPPILTQLAQESGETVLLG
                     MQDAPGYVRLIAKYPSPNDLRYDIEVPRKLPAWNSAIGHVLLGRADEAALEAVLEAAP
                     FGHLGTGVLASREAARAAILAGRDSGTARLVDEASMGVAHLAALIPLGPGQPLAAVEL
                     ACFSQRYHQAPERLFTLLRQAATGIAQAIAGEGAAA"
     gene            6647..8920
                     /locus_tag="VFF98_06380"
     CDS             6647..8920
                     /locus_tag="VFF98_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HZU63289.1"
                     /translation="MKLSVLGGGPTGLYFAISMKLRDPAHQITVYERNRADDTFGWGV
                     VLSDETLAALARNDPASAEAIRSHFAYWDDIRVVRGDEEVTTSGHGFCGIGRLRLLQV
                     LQARAAELGVDIRYEHPVDDAECDRLRRESDLVIAADGLNSRLRTRFADAFCPDVEIR
                     RNKFVWLGTRQTFANAFTFLFEQTEHGWIWAHAYQFDAETATFIAECSEKTWTAFGFG
                     AMSKEENQATLERLFARHLGGHALLSNAHHIRGSAWINFPRVVCGKWHHENMVLLGDA
                     SATAHFSIGSGSKLGMESAIALAERLNQPGDLAAQLDAYQAERALDVLRLQSAARNST
                     EWFEDVERYLGLDMMQFNYSLLTRSQRISHENLRQRDPAWLAEAEGWFQRRAGSNSQR
                     RPMFAPFTLRDLTLKNRVVVSPMAQYKAVDGTPTDWHLVHYAERAKGGAGLVMTEMTC
                     ISAEGRITPGCPGLYAPEHEAAWRRITDFVHAETGAAICCQIGHAGRKGSTRIGWEGM
                     DKPLPEGNWPLLSASALPWSADNATPAAITAAQMEAITAQFVAATQMAERAGFDMVEL
                     HAAHGYLLSAFLSPASNQRTDAWGGSLENRLRWPLDVFAAMRAAWPPAKPMSVRISAT
                     DWLGEDGVTPQEAVQIAAAFRAAGADIVDVSAGQTSTAARPVYGRMFQTPFSDRIRNQ
                     GGIPTMAVGNITEPDHVNSILLAGRADLVCIARPHLADPYWTLHAAVALGERDEGWPL
                     PYLAGRDQAERLATRAP"
     gene            8917..9687
                     /locus_tag="VFF98_06385"
     CDS             8917..9687
                     /locus_tag="VFF98_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63290.1"
                     /translation="MNAPPPTSLAGRRALVTGGGSGAGAAMALALAGAGAEVVICGRR
                     AEPLAAIAAEAPTGAPAIRPCPCDVTDEAAIAALFADHGPFDIVVANAGIAESAPIAR
                     TGLDALERMLAVNLRGVFLPLREAARGMPDGGRFVAVASTAALKGYAYAGAYAASKHA
                     VLGLVRSAALELAPRQITVNALCPGFMDTPMTAQSAARIAARTGRSEEDARAALAATN
                     PMGRLVPPGDVAEALLWLCAPGAGLVTGQAIALSGGEI"
     gene            9705..10127
                     /locus_tag="VFF98_06390"
     CDS             9705..10127
                     /locus_tag="VFF98_06390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU63291.1"
                     /translation="MTSPHTFLHPEGWAAPKGYANGMIATRGRLVALGGLIGWNARQE
                     FEHADLVGQFEQTLRNILAVLAEAGGRPEHLVRLTWYITDRAEYLAHLKELGAAYRRV
                     LGRHFPAMAVVQVAALMEEEAKIEIEATAVLPETEYPA"
     gene            10124..10498
                     /locus_tag="VFF98_06395"
     CDS             10124..10498
                     /locus_tag="VFF98_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZU63292.1"
                     /translation="MSGPVISTPVIATLVLPEAANLHGGAFGGWIMGQCDLAAGLAGR
                     RHAGPCVTKSVQELVFHGPLRVGDALLIEAAIASEGRTSMVLALSGWREDEAGGRALV
                     VSARFVMVCIDAEGRPRPLRGA"
     gene            complement(10495..10908)
                     /locus_tag="VFF98_06400"
     CDS             complement(10495..10908)
                     /locus_tag="VFF98_06400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZU63293.1"
                     /translation="MIPFATTAKVRFAHVDPAGIVFYPRYFEMLNGAVEDWFALGLGQ
                     DFRHLHLDRKVGTPTVKLEATFSAPSFLGDDLDITITPLSLGRSSCPLRVVFACRGEL
                     RLTAEVVLVCMDLNSHQAMAWPEDIRAWITPQLAA"
     gene            complement(10926..11393)
                     /locus_tag="VFF98_06405"
     CDS             complement(10926..11393)
                     /locus_tag="VFF98_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU63294.1"
                     /translation="MEEQDLRPLGDPGSAEFRLDAYPFYLLNRAASRYNTVIEAILRP
                     IGLEIPTWRVLMILGERSPLPIAQVAKSAVINISTMTRIVERMTKAGLVSTLPSATDG
                     RVTELEMTAAGRARLAAARCATAPVYQRIIQGFSAAEFSQMLDFLGRLHDNLA"
     gene            11478..12257
                     /locus_tag="VFF98_06410"
     CDS             11478..12257
                     /locus_tag="VFF98_06410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZU63295.1"
                     /translation="MSTANALGLLAASLASGAVRVVDLTQTLSEDTPLLVLPEPFGQT
                     AAFSRQEISRYDERGVAWHWHNFTVGEHTGTHFDAPVHWVTGKDLPDNTVDRVPVQPL
                     IAPAVVLDFSAACAANPDFLLTAADIAAWEASHGAIPAGSWVLLRTDWSKRDGSAYTN
                     RTADGPHTPGPDASAIEALLARGIIGFGVETIGTDAGQAHLLNPPYPAHTLLHGAGKY
                     GLQCLENLDALPPTGALLIAAPLKIREGSGSPLRVLALVEG"
     gene            12262..>14183
                     /locus_tag="VFF98_06415"
     CDS             12262..>14183
                     /locus_tag="VFF98_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZU63296.1"
                     /translation="MAERSFKGEVQKLTAGAGETFTGEGILAVTKALLQSGVAYVGGY
                     QGSPISHLMDVFADANDLLASLGVHFEASASEATAAAMLAASVNYPLRGAVAWKSVVG
                     TNVASDALSNVASGGVTGGAMIIVGEDYGEGSSIMQERSHAFAMKSQMWLLDPRPNLT
                     SIVEAVEQGFRLSEASHTPVMLQMRVRSCHMTGSFTAKDNVAPAMTVADAAANPMRDT
                     QRIVLPPANFAHEIEKIEQRWPAGIAYVQHNRLNEWFGDSTDTPDIGIIVQGGLYNNL
                     NRAMELMGLADAFGASRLAIYCLNVTYPLIPEEVRDFCAGKQAVLVLEEGQPEFIEHE
                     LNTLVRRADLNTRIIGKDMLPRAGEYSAAVIRDGLAAFLRAYAPQLAPADVAAAPLPA
                     AIAREIPQRPAGFCTGCPERPVFAAMKLVEREIGPTHVSADIGCHLFSILPPFDIGNT
                     TMGYGLGTAGASAFKPKDKRAIAVMGDGGFWHNGLTSGIGNAVFNKDDTVTLIIDNGY
                     SAATGGQDILSSRADNPARVTKNPIAKAVQGVGGQWVRLVERTYDVAKMRDTLRAAMT
                     SKEQGPKVIVAQSECMLNKQRRVKRQQGAALKAGQRVVRERFGVDADTCTGDHACIRL
                     SGCPSLTIRANPDPLR"
BASE COUNT         2353 a         4845 c         4828 g         2157 t
ORIGIN      
        1 cccgccgctg ccggcagggt gatggcgttg aacaggatct tgaaggcgcc ctgggtctgg
       61 ccgcgcaggg caatttccgg ccccaccagc accacccgcc cgcgcccccg gggcagcgag
      121 acgaccgcgc ccgtgccggc cagatattcg gcatgccagg cccagccgga atggagcagc
      181 ggccccgcgc caaaggccag cggcacgctg gcgccgggcg tgccgggctt caggcggaac
      241 acggggcttg catcaaagaa caggtccggc tgcgcggcca ggccccaggc gatggggtcc
      301 tgctggtcgg ccgtggcggt gaggagggcg ccggggatat agaagcggct gcgcggcagc
      361 ggctgggggc ggccatcggc gccagacccg ctcggcgcct cgctcagcgg atcgctcacc
      421 ggcaggtcca tcgccctcgc cagcccggca gaggacgagc ccatcgccac cagcacgccc
      481 ccttgcgtca cgaaaccggc cagcgccggc aacgtgcgtt cggccgaaat agcgcccagc
      541 gaggggcggt attgcgccgg gatatccgcc ggcttgggct gggcattctc gaagcggccg
      601 cggaacatgc cgcccgcccc gccggtgcgc gcattgggga tcgccgcatc gggcaggatc
      661 accacatcga aggcgcgctt cagatggccg gcatccagct gccggggata gacgatgcga
      721 tagggcagct catagccatc gaagatccag cgcagccagc cggtgggatg cagcccgcca
      781 tagagatcca ccagcgccac ccgctgcggc ttgagcgcga tgcgcgggcc ggaaggcgcg
      841 gtggccagcg cctcggccac gatgccaagg ctgcgggccc cctcggccac gatgccgccg
      901 gcagcgccgc cagccggcac ccagatcgcc cccggcgccg ctggcccacc cgccacaggc
      961 aggctggcgc gcatttccgc caccgccaca tgggccttca gcaggcggtt gacgagcagg
     1021 aagctgttgt tctgcgcatg cgacaccagc cagccggccg gatgcgccac agcgcctgcc
     1081 accggcaccg gatcgggggc gatctcaccg gccaccggca cgctgtcggc cgcaaagcca
     1141 tcgagcgcgc gggcaaattc cacgcccatc tggaaggccg gcgtatagcc ggaggcatcg
     1201 gccggcggga ttggcgggcc gccgggatac tggaaattgt tggggtagtg ctggggctcg
     1261 aacatgtcga gcacatgggc gcgataggcc tgcgcggccg gcacgatata ggtgcccgca
     1321 ggccagctgc gcccggccgc ctgaaagggt gcacgggcgc ggcgcacatc cacccccagc
     1381 cgggtgagcg cggtgaggaa acgcgcggcg gtgggaaaat catgctgggc caccggcacg
     1441 atgaaggcgc gcggatcgcg ctgggccgga tcatgcagca cgctgtcgta ataggccttg
     1501 ccgatctcgc tgctgccggt ggtgcgggtg ggccgggggg cctcgccggc agcggcggcg
     1561 gcggcatggg cggccgcgat ccggtcggtc gagatcgtcc actggtcggt gttgccccgg
     1621 gcgatggcat cggcgcccat ctgccagcgg ttgtagagca gcttttcccg gttgccggcg
     1681 gcataggcca gcaccgcgcg ttccaggctc acgctgtagg cgatggattg cgccatcgtc
     1741 cacatctgcg gagccacggg ggcgggcaga tcattgccgg gcacctgccg ctcgggcacg
     1801 aagggaatgc gctctggcac cgggctgccg atgatctcca ccagcaggcc gatggcattg
     1861 tggaaatagg ccgaggtgcg cagattgccg ttataccagg tgtcgtaatg ggcgcccgag
     1921 cgggtggtgg cgcccggctt gccctcggcc agcaggcggc tttgcagcgc cgcgcccact
     1981 tcatcgaggc tggagatcac cagcggctcg tagtgatagt tgaacggatc gcggaacggc
     2041 ggcagaaaca ccaccgtgcc cgaggggccg gtctggtgct ggttgaagat gatctgcggg
     2101 cgccagctct tgtagagcac atcggccatg ttccgcgtct cgaccatctc ggccatgtag
     2161 aaatcgcggt tgttgtcgtg gccgatatag gggtgataga ggcgcggcag cgatgtgaga
     2221 tcgggctcgc gcttgtcggg atcggcaatg cgcatgtacc agtcggccac gaattgctgg
     2281 ccatcggggt tgtctggcac cagcagggtg atcacctgat cgcggatgcg gcgcgcctcg
     2341 gcatcctcgg cggccaccag atcgtagctt tgctggatca gcgcggtctg ggcttccacc
     2401 tcggcggcat gcaggccgcc atccacccac accagcgccc tgccctcgcc ggccagctgg
     2461 cgcgcggcgg ccgcgtcgat cccctcggcg gtgcccagct ggcggctgat ctcgcggtaa
     2521 tggtcgagcc gggccaggtt ttcgggcgag gagacgaccg ccatgatctg gtggcgcccc
     2581 tgggtcgtct ggccaatgtc gatcagccgg atgcgatcgg aacgggccgc cagtgcgcgc
     2641 cagtaacgca gcatgtcctc gtaggagggc acataatggt tctcgcccat catgtggccc
     2701 agcaccgcct ggggcgtggg caggggcgcg gcagcggggc cggcatcagg cttggccagg
     2761 gccggcaggg gggcaagcgc cagcgcggca gcgccagcca ggaaaagcgt gcggttcatc
     2821 gatctatcgc cgtgtgcgga tctggcgcgg gcaccagcag gatggtgaca agcgagaggg
     2881 cggccgcccc catcacataa aaggccgggg caaaggccag gccagtggca tgcaccagca
     2941 gcgtgccgat gaacggggca aagccgccaa acagcatcac cgcgccatta tagccaaccg
     3001 acagcgccgt gtagcgcagg cgcgtgggca gtagctcgca cagataggtg ggtgccgtgc
     3061 ccgagaacag gccggaaaac aggccggcaa cgcccgttgc caccagcacg gcggccgggc
     3121 tgcgggcctg ggccatcagc acgaacagcg gcaggctgca gataaggaaa ccggccgcgc
     3181 tggccgccat gaaaggcttg cggcccacct tgtcggacag ggcgccggcc agcgggatga
     3241 cggcgatatt gaccagcgcg cccaccagcg tgccggtaag cgccgcgcgc gccggcagat
     3301 gcagcacctg cccggcaaag ctgaccagga acgtgccata ggtgtagccg gccaccgtcc
     3361 acaccatcac catcgcgccg atcgtcagca tggtgcggcg ctgggtggtg aaggcctcac
     3421 gcaaggggtg caaagggcgc tcggcggcgg tttcggcaac aaagccgggg gtttcggcca
     3481 cgttgcggcg cagatagaag cccagcggcg cgatcagcac gcccagcacg aaaggcaccc
     3541 gccaacccca ggcctgcatc gcctgcgcac tgagcgcggc attgaggccc agcgccacca
     3601 gcaccgccgt caccaccgcc acattggtgc tcatctgctg cagcgcgccg aacaggccgc
     3661 gccggccggc cggcgcatat tcgaccagaa aggtagtggc cccgccccat tcgccgccgg
     3721 cggcaaagcc ctgcaccagc cggcacagca acagcagcgc gggcgcggcc aagccaatgc
     3781 gggcataatc gggcaccagc cccgtggcgc aggtcgatcc ggccatcatc agcatggtga
     3841 gcaccagcgc cgacttgcgg cccagcctgt cgcccatgcc gcccagcacc atcgagccca
     3901 ccggccgcat cagaaagccc acgccatagg tggcaaagct ggacagcacc gaggtgagcg
     3961 gatcggccgc ggggaacatc gccttgccga tctgcgcggc gaaatagcca tagatgatga
     4021 aatcgaacag ttcgaaccag ttgcccacca tggccgaacc cagcgcgcgg gccagttcgg
     4081 cccggccggg cacggggcga ggaatggacg cgttcatggc gcgtggcttt cggcagcagc
     4141 agcggcatgg gcggggcccg aaacggggcc cggcagcggg cgcggcacat gcggcatgat
     4201 caccacctgc ccgccgcgca ccatcacctg cccatccttg acgaccagcg ccacgtgatg
     4261 cagcgcatcg atatcctgat cgggccggcc ggccaccacc agcacatcgg ccagcatgcc
     4321 cggcgcgatg cggcccagtt cgtcgcccat gtcgagcagg cgggcattgg tgagcgtggc
     4381 cgcctgcagc gcctgcggga tggtgtagcc gcccttcacg aaccacttca gctcggccag
     4441 atagatgttg ggcgtcatcc ggttggcatc gccgatcgtg tcggtgccca cgcccatgac
     4501 gatgccggcc gccttcatct tgcggaacac ggcgaaattg tcgtccgagg tcatggtgaa
     4561 gcgccgcgag gtggagcccc agaagccccc tgcccggtcg atcacattct gatagacctg
     4621 cagcgtgggc acggccgccg tgtggcggcg ggccatggcg gcaatcgtct cgtcggtgcg
     4681 cggcagcggg tgctcgatca tgtcgatgcc ggcatccacc gccatcttcg tgtagatcgt
     4741 ctcgcagtcg caggtcacct tcaggcccag ccggtgcgcc tcgtccaccg ccgcctgcac
     4801 ttcatcgggc gcgaaatggc tggccagctt gatcaccgtg gcgcccttgc ggaaggtttg
     4861 ccgcacggcc gcgcgccagg catcggcgcc atcgcgctgc caggcataca tgtcgccatg
     4921 ggtggggccc acggggcgct ctgtggcatg gccgccggtg ccggtgatga tgtggccggc
     4981 agtgaacacg cgcggggcag gaatggcatc ggcagcggcg gcatcggcca gccggtaggg
     5041 cgcatcgccc acgccgttga gatcgcgcac cgatgtgaag cccgcctcga tgaaccagcg
     5101 caggttgcgg atgccgcgca gcacgccatc ggcctcgctg gcctgcacgt cgatcggcgt
     5161 gtcgctgtcg gggtaggtga tgtgcacatg catgtcgatg aggccgggca tgacgaattg
     5221 ccccgccaca tcgatcacct gcgcatcggc gggccaatcc gcggcgccgg gcggcagcag
     5281 gcgggcgatg cgcttgccgg cgatcaccac ggtgagcggg cgcacggcaa ggctctcgct
     5341 atcaaacacc aggccgccgc gcagcacggt gacgggctgg gcgccggcca ccggccgttc
     5401 gggcacgcgg cggggggtgt cgtcggtgtt ggtgccgggc gcttcccaca ggcggcggtc
     5461 ggtcagcggc acggaggggt ggtcctgcgc ggcggcgggg gttggcagca gcagggcggc
     5521 ggccagcggc agggcggcag ccatgttctt gcgggaaatg ctcatgcggc ggcgccttcc
     5581 ccggcaatgg cctgggcgat gccggtggcg gcctggcgca gcagggtgaa cagccgttct
     5641 ggcgcctggt gatagcgctg gctgaaacag gccagctcca ccgccgccag cggctggccc
     5701 ggccccagcg gaatgagcgc ggccagatgc gccacgccca tgctggcctc gtccaccagc
     5761 cgcgcggtgc cgctgtcgcg cccggccagg atcgccgcgc gggcggcctc gcggctggcc
     5821 agcacgccgg tgcccagatg gccgaagggc gcggcttcca gcaccgcctc cagcgcggcc
     5881 tcatcggcgc ggcccagcag cacatggccg atcgccgaat tccacgccgg cagcttgcgc
     5941 ggcacctcga tgtcgtagcg cagatcgttg ggcgagggat atttggcgat cagccgcaca
     6001 tagccgggcg catcctgcat gcccagcagc accgtctcgc ccgattcctg cgccagctgc
     6061 gtaaggatgg gcggcgccac ccgcagcagc cggccatggc gggcaaagcc atcgcggcgg
     6121 atcatgtcgt tcagctgata ggcgtcgtta tcgtccaccc gcacgaggta gccgcgcttg
     6181 tgcagcgtgc gcagcaggcc cagcgcgctg ctcttgggca ggctgaggcc gcgcgccact
     6241 tcgctcagcg tcatcgcgtc gcaacgggtg gagaacattt ccagcatatc gaggacgcgg
     6301 gcggcggatt tgatttcgtc ggtcatatat gtgaacaact attcctatct gtgaacaatg
     6361 tttcatgatt cggctgagat gtcaaaccgg caaagaccca tgattttgcc gataaatagc
     6421 acgaatatcc cataaaatac ccataattcg ggatatccat gcccatcacc ggcagcatgc
     6481 ccgccgcccg cccatggctt atgcgtgcgc catgacgcac acggcgaaca ggctttcatc
     6541 catcatcagc ggccatcgcc atggcgaata tatttcaagc ttgaagtata atcccgccct
     6601 gcgtactctg cccgcaacga cagcacccag gctgaaggag caggcgatga aactatccgt
     6661 gctcggcggc gggcccaccg gcctctattt cgccatttcg atgaagctgc gcgatccggc
     6721 gcaccagatc accgtttacg aacgcaaccg cgccgacgac accttcggct ggggcgtggt
     6781 tctctccgac gagacgctgg ccgccttggc ccgcaacgac cccgccagcg ccgaggccat
     6841 ccgcagccat ttcgcctatt gggacgatat ccgcgtggtg cggggcgacg aggaagtgac
     6901 gaccagcggc cacggctttt gcggcatcgg ccggctgcgg ctgttgcagg tgctgcaggc
     6961 gcgcgcggca gagctgggcg tggatatccg ctatgaacac ccggtggatg atgccgaatg
     7021 cgaccggctg cgccgcgaaa gcgatctggt gatcgcggcc gacgggctga acagccgcct
     7081 gcgcacccgc tttgccgatg ccttctgccc cgatgtcgag atccgccgca acaaattcgt
     7141 ctggctgggc acgcggcaaa ccttcgccaa cgccttcacc ttcctcttcg agcagaccga
     7201 gcatggctgg atctgggccc atgcctatca gttcgatgcc gaaacggcga cgttcatcgc
     7261 cgaatgcagc gagaaaacct ggaccgcctt cggctttggc gcgatgagca aggaggagaa
     7321 ccaggccacg ctcgaacgtc tcttcgcgcg ccatctaggc ggccacgcgc tgctgtcgaa
     7381 tgcccatcac atccgcggct cggcgtggat caactttccc cgcgtggtgt gcggcaagtg
     7441 gcaccacgag aacatggtgc tgctgggcga tgcctcggcc acggcgcatt tctccatcgg
     7501 ctcgggctcc aagctgggga tggaaagcgc cattgccctg gccgagcggc tgaaccagcc
     7561 gggcgatctg gccgcccagc tcgatgccta tcaggccgag cgcgcgctgg atgtgctgcg
     7621 cctgcaatcg gccgcgcgca attccaccga atggttcgag gatgtcgagc gctatctcgg
     7681 gctcgacatg atgcagttca actattcgct gctcacccgc agccagcgca tcagccacga
     7741 gaacctgcgc cagcgcgatc cggcctggct ggcagaggcg gaaggctggt tccagcgccg
     7801 cgccggatcg aacagccagc gccggccgat gttcgcgccc ttcaccttgc gcgatctcac
     7861 gctgaagaac cgcgtcgtcg tctcgcccat ggcgcaatac aaggcggtgg atggcacgcc
     7921 gacagactgg cacctggtgc attatgccga acgcgccaag ggcggcgccg gcctggtgat
     7981 gaccgagatg acctgcatca gcgccgaggg ccgcatcacg cccggctgcc cgggccttta
     8041 tgcgcccgaa catgaagcgg cgtggcggcg catcaccgat ttcgtgcatg ccgaaaccgg
     8101 cgcggcgatc tgctgccaga tcggccatgc cgggcgcaag ggctccaccc gcatcggctg
     8161 ggaaggcatg gacaagcccc tgcccgaggg caactggccg ctcctctcgg cctcggccct
     8221 gccgtggagc gcggacaatg ccacgcctgc cgcaatcacc gccgcgcaga tggaagcgat
     8281 caccgcgcag ttcgtggcgg ccacgcagat ggcagagcgc gccggcttcg acatggtgga
     8341 actgcatgcc gcccatggct atctgctgtc cgccttcctg tcgccggcct ccaaccagcg
     8401 caccgatgcc tgggggggca gcctggagaa ccgcctgcgc tggccgctgg acgtgtttgc
     8461 cgccatgcgc gccgcctggc cgccggccaa gccgatgtcg gtgcgcattt ccgccaccga
     8521 ctggctgggc gaggacggcg tgaccccgca ggaagccgtg cagatcgccg ctgccttccg
     8581 cgccgccggg gccgatatcg tcgatgtgtc ggccgggcag accagcacgg ctgcgcgccc
     8641 cgtctatggc cgcatgttcc agaccccgtt ttccgatcgc atccgcaatc agggcggcat
     8701 tcccaccatg gcggtgggca atatcaccga gccggaccat gtgaactcga tcctgctggc
     8761 cggccgcgcc gatctggtgt gcattgcccg cccgcatctg gccgatccct attggacact
     8821 gcatgccgcc gtggcgctgg gcgagcgcga cgagggctgg ccgctgccct atctggccgg
     8881 gcgcgatcag gccgagcggc tggccacgcg cgcgccatga acgcgccccc ccccaccagc
     8941 cttgccggcc gccgcgcgct ggttaccggc ggcggcagcg gcgcgggcgc ggccatggcg
     9001 ctggcgctgg ctggcgctgg cgccgaggtg gtgatctgcg ggcggcgggc agaaccgctg
     9061 gccgccatcg ccgccgaagc ccccaccggc gcgcccgcca tccgcccctg cccctgcgat
     9121 gtgaccgatg aggccgccat tgccgcgctg tttgccgatc atggcccgtt cgacatcgtc
     9181 gtcgccaatg ccggcatcgc cgaaagcgcg ccgatcgccc gcaccggcct cgatgcgctg
     9241 gagcggatgc tggcggtgaa cctgcgcggc gtgttcctgc ccctgcgcga ggcggcccgc
     9301 ggcatgcccg atggcgggcg ctttgtggcg gtggcctcca ccgcggcgct caagggctat
     9361 gcctatgccg gcgcctatgc cgccagcaag catgcggtgc tggggctggt gcgttcggct
     9421 gcgctggaac tggcgccgcg gcagatcacc gtcaacgccc tgtgcccggg ctttatggac
     9481 acgccgatga ccgcccaatc cgccgcccgc atcgccgcca gaaccgggcg cagcgaggaa
     9541 gacgcccgcg ccgcccttgc cgccaccaat ccgatgggcc ggctggtacc ccccggcgat
     9601 gtggccgaag cattgctgtg gctgtgcgcg ccgggcgccg ggctggtgac aggccaggcc
     9661 atcgcccttt ccggcggcga aatctgaaag gaccttcccc cgacatgacc tctccccaca
     9721 ctttcctcca ccccgaaggc tgggccgcgc ccaagggcta tgccaacggg atgatcgcca
     9781 cgcgtggccg gctggtggcg ctgggcggcc tgattggctg gaacgcgcgg caggaattcg
     9841 agcatgccga tctcgtcggc cagttcgagc agaccttgcg caacatcctc gccgtgctgg
     9901 cagaggccgg cgggcgcccg gaacatctcg tgcggctgac ctggtacatc accgacaggg
     9961 ccgaatatct ggcgcatctg aaagagctgg gcgcggccta tcggcgtgtg ctggggcggc
    10021 atttcccggc gatggcggtg gtgcaggtgg cagcactgat ggaagaggaa gccaagatcg
    10081 agatcgaggc aaccgccgtg ctgccagaaa cggagtatcc ggcatgagcg gcccggtgat
    10141 ttccaccccg gtgattgcca cgctggtgct gcccgaggcg gccaaccttc acggcggcgc
    10201 ctttggcggc tggatcatgg gccagtgcga tctggccgca gggctggccg ggcgccgcca
    10261 cgcgggcccc tgcgtcacca aatcggtgca ggaactggtg tttcacggcc ccttgcgcgt
    10321 gggcgatgcg ctgctgatcg aggccgccat cgccagcgag gggcgcacgt ccatggtgct
    10381 cgccctgtcg ggctggcgcg aggatgaggc gggggggcgg gcgctggtcg tctcggcgcg
    10441 cttcgtcatg gtctgcatcg atgccgaggg acgcccgcgc ccgctgcgcg gtgcctaggc
    10501 ggccagctgc ggggtgatcc aggcgcggat atcctccggc caggccatcg cctgatggct
    10561 gttgagatcc atgcacacca gcaccacctc ggcggtcagc cgcaattcgc cccggcaggc
    10621 aaacaccact ctcagcgggc aactgctgcg ccccagcgac agcggcgtta tggtgatatc
    10681 gagatcatcg cccaggaaac tgggagcgct gaaggtggcc tccagcttca ccgtgggcgt
    10741 gcccaccttg cgatcgaggt gcaggtggcg gaaatcctgc cccagcccca gggcgaacca
    10801 gtcttccacc gccccgttca acatctcgaa atagcgcgga tagaagacga tgccggccgg
    10861 atcgacatgg gcaaagcgca ctttggcagt ggtggcaaag gggatcatgg caaaggcgct
    10921 cccggtcagg cgagattgtc gtgcaggcgg ccgaggaaat cgagcatctg gctgaattcc
    10981 gcggcggaaa agccctggat gatgcgctgg tagacaggcg ctgtggcaca gcgcgccgcg
    11041 gccaggcggg cgcggccggc cgcggtcatc tccagctcgg tcacgcggcc gtcggtggcg
    11101 ctgggcaggg tgctcaccag cccggccttg gtcatccgtt caacgatgcg ggtcatggtg
    11161 gaaatgttga tcaccgccga tttggccacc tgggcgatcg gcagcggcga tcgctcgccc
    11221 aggatcatca gcacccgcca ggtgggaatt tccaggccga tcggccgcag gatcgcctcg
    11281 atcaccgtgt tgtagcggct ggccgcgcgg ttcagcagat agaagggata ggcatccagc
    11341 cggaattcgg ccgaaccggg atcgccaagg gggcgcagat cttgctcttc catcacgctg
    11401 tcatcgccta aacattttgc ttgcatatgc aagttatatc gatagcctgt cgcctcacat
    11461 gcttcgcagg agatcccatg tccactgcca atgcgctggg ccttttggcc gcctccctgg
    11521 cctctggcgc cgtgcgcgtc gtcgatctca cgcagacgct gtcggaagac acgccgctgc
    11581 tggtgctgcc cgaacccttc ggccagacag ccgccttctc gcgccaggaa atctcgcgct
    11641 atgatgaacg cggcgtggcc tggcactggc acaatttcac cgtgggcgaa cacaccggca
    11701 cccatttcga cgcgccggtg cattgggtga ccggcaagga cctgcccgac aatacggtgg
    11761 accgcgtgcc cgtgcagccg ctgatcgcgc cggccgtcgt gctcgatttt tccgccgcct
    11821 gcgccgccaa ccccgatttc ctgctgaccg ccgccgatat cgccgcctgg gaagccagcc
    11881 atggcgccat tcccgccggc agctgggtgc tcttgcgcac agactggtct aagcgcgacg
    11941 gaagcgccta caccaaccgc accgccgatg gcccccacac ccccggcccc gatgccagcg
    12001 ccatcgaagc gctgctggcg cgtggcatca tcggctttgg cgtggagacg atcggcaccg
    12061 atgccggcca ggcccatttg ctgaacccgc cctatccggc ccacacgctg ctgcatggcg
    12121 ccggcaaata tggcctgcaa tgcctggaaa acctggatgc gctgccgccc acaggcgcgc
    12181 ttctcatcgc cgcgccgctg aagatccgcg aagggtctgg cagccccttg cgcgtgctgg
    12241 cgctggtgga aggctgaacc gatggcggaa cgttccttca agggcgaagt gcagaagctg
    12301 accgccggcg ctggcgagac cttcaccggc gagggcattc tggcggtcac caaggcgctg
    12361 ctgcaatcgg gcgtggctta tgtcggcggc tatcagggct cgcccattag ccacctcatg
    12421 gatgtgtttg ccgatgccaa tgatctgctc gccagccttg gcgtgcattt cgaggccagc
    12481 gccagcgagg ccaccgccgc cgcgatgctg gcggccagcg tcaactatcc cttgcgcggc
    12541 gcggtggcgt ggaaatcggt ggtgggcacc aatgtcgctt ccgatgcgct gtcgaacgtg
    12601 gccagcggcg gcgtgaccgg cggggcgatg atcatcgtcg gcgaggatta tggcgagggc
    12661 tcctcgatca tgcaggagcg cagccatgcc tttgccatga aaagccagat gtggctgctg
    12721 gacccgcgcc ccaacctcac cagcattgtg gaagcggtgg agcagggttt ccgcctgtcg
    12781 gaagcctcgc acacgccggt gatgctgcaa atgcgcgtgc gctcctgcca catgaccggc
    12841 agcttcaccg ccaaggacaa cgtggcgccc gcgatgaccg tggccgatgc cgccgccaac
    12901 cccatgcgtg acacccagcg catcgtgctg ccgccggcga atttcgccca tgagatcgag
    12961 aagatcgagc agcgctggcc cgccggcatc gcctatgtgc agcacaaccg cctgaacgaa
    13021 tggtttggcg acagcaccga cacgcccgat atcggcatca tcgtgcaggg cgggctctac
    13081 aacaatttga accgcgcgat ggagctgatg ggcctcgccg atgcctttgg cgccagccgc
    13141 ctcgccatct attgcctcaa cgtcacctat ccgctgatcc ccgaggaagt gcgcgatttc
    13201 tgcgccggca agcaggcggt gctggtgctg gaggaaggcc agcccgaatt catcgagcat
    13261 gagctgaaca cgctggtgcg ccgcgccgat ctcaacaccc gcatcatcgg caaggacatg
    13321 ctgccgcgcg cgggcgaata ttccgccgcc gtgatccgcg acgggctggc cgccttcctg
    13381 cgcgcctatg cgccgcagct ggcgccggcc gatgtggcgg ccgccccgct gcctgccgcc
    13441 attgcccgcg agattcccca gcgccccgcc ggtttctgca ccggctgccc ggagcgcccg
    13501 gtgttcgccg cgatgaagct ggtggaacgc gagatcgggc cgacgcatgt ctcggccgat
    13561 atcggctgcc acctgttctc catcctgccg ccgttcgaca ttggcaacac caccatgggc
    13621 tatggcctgg gcacagccgg cgccagcgcc ttcaagccca aggacaagcg cgccatcgcg
    13681 gtgatgggcg atggcggctt ctggcacaat gggttgacga gcggcatcgg caatgcggtg
    13741 ttcaacaagg acgacaccgt caccctcatc atcgacaatg gctattcggc cgccaccggc
    13801 gggcaggata tcctctccag ccgggccgac aaccccgcgc gggtgacgaa aaaccccatc
    13861 gccaaggcgg tgcagggggt gggcggccaa tgggtgcggc tggtcgagcg cacctatgac
    13921 gtggccaaaa tgcgcgacac cttgcgcgcg gcgatgacca gcaaggagca gggccccaag
    13981 gtcatcgtcg cccagtcgga atgcatgctc aacaagcagc ggcgagtgaa gcgccagcag
    14041 ggcgcggcgc tgaaggcggg ccagcgggtg gtgcgcgaac gcttcggcgt ggatgccgat
    14101 acctgcacgg gcgatcatgc ctgcatccgc ctctcgggct gcccctcgct gacgatccgc
    14161 gccaaccccg atccgctgcg cca
//
LOCUS       DASRYE010000116         7294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_130156,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000116 DASRYE010000000
VERSION     DASRYE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130156"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFF98_06420"
     CDS             <1..913
                     /locus_tag="VFF98_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU63297.1"
                     /translation="PAAEPVAEPRIEAAPAVVAPPAPAPEVHLPPVAVAQDAPAPEYA
                     AIPADQPAPARRWQPTRFHAVDRERLGEAGALPGAGGASVQQEEFRIVKRQLLLQAED
                     LRRQGGGGAAQRVMICSPHPAEGKTFCAVNLALSIAAEKDSEVLLVDLDLARLSVLEM
                     LGLPREPGLMDALADPAIDLRDCVIATDIPGLSVLPGGRATALDTEYLASARAHRLLD
                     TLTEDAPNRIVLFDTPPALVASLPAEVAKLVGQVVLVVMADKTSASAINDAASLLAGC
                     PNIQLLLNGVRFSPSGRRFGSYYGYQG"
     gene            919..2712
                     /locus_tag="VFF98_06425"
     CDS             919..2712
                     /locus_tag="VFF98_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZU63298.1"
                     /translation="MQKVSRLAAIAPVATIALVLAGPALAQQAALQQAAPARMAYLPT
                     EPVQPAYAPAQGAPLGEADGAEDLGGAEGQDGPSTSDAAHPKQGRSARRFTIRPYIEA
                     MQVFDADVAGDHEVDTYTTLAAGADGQLNGRNTKAAFSVRYEHRIGEHNAGDGDGVSG
                     MLRGRSRLGTDGLHLDYGAMAQRMHLDAGGAATADTGIAAADTQLYSAFIGPSFAGKL
                     GALRFEDHYHYGYTAVSGGTAGVGGAGGTDVLSHAQVHDLGGSLGNRPGEGLPVGLSV
                     EGGYHEEDVSNLGQRFTDAYGQGRLTVPVAPHLALTGALGYEHVEVSAHDALYDASGN
                     VLYDSAGRLETDWSSPRHIAFDTSGLTWDAGLIWRPSRRTSLEAHVGRRYGALGGYGS
                     FSYQPDPRNSFNLVVYNEVGSSGGAMVSGLVGTSTDFSAIRDAISGALVGCAASVQGT
                     ACIAGASGSLSSAIGRRRGVLANWGINFGRVQAGIGAGYDRREYLTGSGTVLGSLNGE
                     VDQYEWVAAYIADRLSARASVQGTVDLYHYETGLAGSGNVSAIRVSGVYQYMLTRHLT
                     ASAALAINGMSREALDDLWATSGALAMRYNF"
     gene            2731..3882
                     /locus_tag="VFF98_06430"
     CDS             2731..3882
                     /locus_tag="VFF98_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HZU63299.1"
                     /translation="MFDDFYGFTARPFQLTPDPAFYFESTTHRKALSYLGFGLAQGEG
                     FIVITGEVGAGKSTLVAHLMATIDRARLTAGQIVTSRLDGEEMVHLAAQAFGLKVPGH
                     DKASALGAIEAFLHAEARAGRRCLLVVDESQNLSVEALEELRMLSNFQLGSHPLLQIL
                     LLGQPEFRESLEGHPRLEQLRQRVIAAHHLEAMAPEELEPYVQHRLRLVGWQGRPGFA
                     ADLFGALHRATGGIPRRVNQVLNRLLIFGAVERTDYIDCAMLEQVLADLGSDGLRPLD
                     HNTRVASPVAEPSPVPAAAAGLQALQGAIAQRDAQIAELQQAVIELASAAEASGQPVQ
                     GEVARAIGLIEERLTALENRLSEQEHTLRHTLTMLIEWMERDDAQRVAA"
     gene            3879..4760
                     /locus_tag="VFF98_06435"
     CDS             3879..4760
                     /locus_tag="VFF98_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HZU63300.1"
                     /translation="MTGPAPASVINALSVDVEDWFQVGAFEQVIDRADWESLESRVEN
                     NCLRILDLFAECGVKATFFTLGWVARRQPALMRRIVSEGHELASHGWDHARVFTLDAA
                     RFAEDLRRARQALEDAAGVAVIGYRAPSFSIDARTPWAHPVLAEQGYAYSSSVSPIAH
                     DHYGWRAAPRFAFQPVAGSALLEVPVTSVELGGRRFAAGGGGFFRVLPYGFSRWAIRR
                     VNGHDGLPAITYFHPWEIDPGQPRVAGAPLRSRLRHYTGLGAMEGKLRRLLGDFRWGR
                     MDELFCPVAGEDVSAAA"
     gene            4757..5833
                     /locus_tag="VFF98_06440"
     CDS             4757..5833
                     /locus_tag="VFF98_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HZU63301.1"
                     /translation="MNAPWLSTRGGRARLVDPAGAEGARLEAFVASHPEGTLFHRPAW
                     LCAVARGAGHRAAMLVLEGAQEIAALLPVNLIHSPIFGRVIASSGFGVGGGLLALPGV
                     DVAPLVAAVEELAARWSCPGVELRGGPLPEGRPGWRIRADAHAGFIRPLAADDEAELL
                     AIPRKQRAEVRKGLAADLAITIGSSAADRAAHYAVYAESVRNLGTPVFPRALFAAMLD
                     GFGADADILTVSHQGVPVASVLSFYHAGAVLPYWGGGTLAARGLRANDRMYFELMRHA
                     RARGCTRFDFGRSKTGSGAFAFKRNWGFDPAPLAYASWQAPGQPLRDADPTSAVHARR
                     IDLWKRLPLPLANLLGPFIARGLG"
     gene            5830..7074
                     /locus_tag="VFF98_06445"
     CDS             5830..7074
                     /locus_tag="VFF98_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HZU63302.1"
                     /translation="MISGEILMLAHRIPFPPDRGDKIRSHHILKKLVRMAPVHVACFA
                     DTDADLAEEVELAAVAHSYRLIRRAKSLFTAGAQALLMHRPVSLTAFHDPGLADYVKR
                     LLATGRVRTIYVFSGQMAQYVPADFTGRLVIDFVDVDSAKFEAYAARHRGPMGWLYAR
                     EARLLSAEEQRQAARADAVLLVSHAEADLFASRMPPGAATQVRVLGNGVDADAYDPAQ
                     QFAATPMAGHGAPRLIFTGQMDYPPNIEAAMRMARRIMPLVRAQCPEASFHVVGRQAG
                     EKVLALDGLHGTKVWGEVADIRPWLRAADMAVVPLDIARGVQNKVLEAMAMGLPVVAS
                     HAAATGINAIAGHHFMVADNDTAIAAAVVSLAHDPHLMFRMGQEGRRFVIEHQGWQRV
                     LAPLAGLLSSAPPPPREPRAGA"
     gene            7098..>7294
                     /locus_tag="VFF98_06450"
     CDS             7098..>7294
                     /locus_tag="VFF98_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HZU63303.1"
                     /translation="MPAQAAGVDWRMALGLWAAAAGALVMLARAEWLAMAGQWLGSST
                     YEHILLVPAIILWLVAERRPSL"
BASE COUNT         1009 a         2416 c         2658 g         1211 t
ORIGIN      
        1 accggcggcc gaacctgtgg ccgaaccccg gatcgaggcg gcgcccgcag tggtggcgcc
       61 gcccgcccct gcgcccgagg tgcacctgcc cccggtggcc gtggcgcagg atgcacccgc
      121 cccggaatat gcggccatcc cggcagatca gccggccccg gcccggcgct ggcagcccac
      181 gcgcttccac gcggtggatc gtgagcggct gggcgaggcg ggcgcgctgc ccggcgctgg
      241 cggcgccagc gtgcagcagg aagagttccg catcgtcaag cgccagttgc tgctgcaggc
      301 ggaagacctg cgccggcagg gcggcggcgg ggcggcgcag cgggtgatga tctgctcgcc
      361 ccacccggcc gagggcaaga ccttctgcgc ggtcaatctg gcgctttcca tcgcggcgga
      421 aaaggacagc gaggtgctgc tggtcgatct cgatctggcg cggctttcgg tgctggagat
      481 gctgggcctg ccgcgtgagc cggggctgat ggatgcgctg gcggacccgg cgatagacct
      541 gcgcgactgt gtgatcgcca ccgacattcc cggcctgtcg gtgctgccgg gcgggcgggc
      601 caccgcgctc gataccgaat atctggcctc ggcccgcgcc catcgcctgc tcgatacgct
      661 gactgaggat gcccccaacc gcatcgtgct gttcgacacg ccgccggcgc tggtcgcctc
      721 gctgccggcg gaagtggcca agctggtggg ccaggtggtg ctggtggtga tggccgacaa
      781 gacctcggcc agcgctatca acgatgccgc ctcgctgctg gccggctgcc ccaatatcca
      841 gctgctgctc aacggggtgc gcttcagccc cagcgggcgg cgcttcggct cctattacgg
      901 ctatcaggga tgagcgggat gcaaaaagtt tcgcgcctgg cggccattgc ccctgttgcc
      961 accatcgccc tggtgctggc cggccccgcc ctggcccaac aggcagccct gcagcaggca
     1021 gcgcccgcgc gcatggccta tctgcccaca gagccggtgc agcccgccta tgccccggca
     1081 caaggcgcgc ccctgggcga ggccgatggc gccgaggatc tgggcggcgc cgaagggcag
     1141 gatggtccca gcaccagcga tgccgcccac cccaagcagg gccgctctgc ccggcgcttc
     1201 accatccgcc cctatatcga ggcgatgcag gtgttcgatg ccgatgtggc gggcgaccat
     1261 gaggtggata cctacaccac cctggctgcc ggcgccgatg gccagctgaa cgggcgtaac
     1321 accaaggcgg ccttctcggt gcgctatgaa caccgcatcg gcgagcacaa tgccggcgat
     1381 ggcgatggcg tttcgggcat gctgcgcggg cgcagccggc tgggcacgga tgggctgcac
     1441 ctcgattatg gcgcgatggc ccagcgcatg caccttgatg ccggcggcgc ggccacggcc
     1501 gacaccggca tcgccgcggc cgacacccag ctctattccg ccttcatcgg cccttccttt
     1561 gccggcaagc tgggcgcgct gcgcttcgag gaccattacc actatggcta caccgccgta
     1621 tcgggcggca cggcgggcgt gggcggggca gggggcaccg atgtgctctc gcacgcgcag
     1681 gtgcatgatc tgggcggctc gctgggcaac cggcccggcg agggcctgcc ggtggggctg
     1741 agcgtggagg gcggctatca cgaggaggat gtctccaacc tcggccagcg cttcaccgat
     1801 gcctatgggc agggccgcct caccgtgccg gtggcaccgc atctcgccct taccggcgcg
     1861 ctgggctacg agcatgtgga agtttccgcc catgatgccc tctatgatgc cagcggcaac
     1921 gtgctttacg acagtgctgg ccggctggag acggactggt cctcgccgcg ccacatcgct
     1981 ttcgatacca gcgggttgac gtgggatgcc gggctcatct ggcggcccag ccggcgcacc
     2041 agcctcgagg cgcatgtggg ccggcgctat ggggcgctgg gcggctatgg cagcttttcc
     2101 tatcagcccg atccgcgcaa cagcttcaac ctggtggtct ataacgaggt gggcagttcc
     2161 ggcggggcga tggtttccgg cctggtgggc accagcaccg atttttccgc catccgcgat
     2221 gcgatcagcg gcgcgctggt gggctgcgcg gccagcgtgc agggcacggc ctgcatcgcc
     2281 ggggcctcgg gctcgctcag ctcggcgatc gggcggcggc gcggggtgct ggccaattgg
     2341 ggcatcaatt tcggccgcgt acaggccggc atcggcgctg gctacgaccg gcgcgaatat
     2401 ctcaccggca gcggcaccgt gctgggcagc ctcaacggcg aggtggacca gtatgaatgg
     2461 gtggccgcct atatcgccga ccgcctgtct gcccgcgcca gcgtgcaggg cacggtggat
     2521 ctttatcatt atgaaacggg gcttgcaggc agcggcaatg tcagtgcaat ccgggtcagc
     2581 ggcgtgtatc aatacatgct cacccgccat ctgacggcaa gtgcggcgct ggccatcaat
     2641 ggcatgtcgc gcgaggcgct ggatgatctg tgggccacat ctggcgcttt ggccatgcgc
     2701 tacaacttct aggggccggg agacgcggcg atgttcgacg atttctatgg ctttacggcc
     2761 cgcccgttcc agctgacgcc ggacccggcc ttctatttcg aaagcaccac ccacaggaag
     2821 gcgctctcct atctgggctt tggcctggcg cagggggaag gcttcatcgt catcaccggc
     2881 gaggtggggg cgggcaagtc cacgctggtg gcccatctga tggcgacgat cgaccgcgcg
     2941 cggctgaccg ccgggcagat cgtcaccagc cgcctcgatg gcgaggaaat ggtgcatctg
     3001 gcagcccagg ccttcgggct gaaagtgccg gggcacgaca aggcctcggc gctgggcgcg
     3061 atcgaggcct tcctgcatgc cgaggcgcgg gccgggcggc gctgcctgct ggtggtggat
     3121 gaaagccaga acctttcggt ggaagcgctg gaagagctgc gcatgctctc caatttccag
     3181 ctgggcagcc acccgctgct gcagatcctg ctgctgggcc agcccgaatt ccgcgagagc
     3241 ctggagggcc atccgcgcct tgaacaattg cgccagcggg tgatcgcggc gcaccacctc
     3301 gaggcgatgg cgcccgagga gctggaaccc tatgtgcagc accggctgcg gctggtgggc
     3361 tggcaggggc ggccgggctt tgcggccgat ctgttcggcg cgctgcaccg ggccacgggc
     3421 ggcattccgc ggcgggtgaa ccaggtgctc aatcgcctgc tgatcttcgg cgcggtggaa
     3481 cgcaccgatt atatcgattg cgccatgctg gagcaggtgc tggcggatct gggcagcgat
     3541 ggcctgcgcc cgctcgatca taacacccgc gtggcgtccc cggtggcaga gccttcgccg
     3601 gtgccggcgg ccgcggccgg cctgcaggcg ctgcagggcg ccattgccca gcgcgatgcc
     3661 cagatcgccg aactgcagca ggcggtgatc gaactggcca gcgcggccga ggccagcggc
     3721 cagccggtgc agggcgaggt ggcgcgcgcc atcggcctca tcgaggagcg gctgacggcg
     3781 ctggaaaacc gcctttccga gcaggaacac accttgcgcc acacgctgac catgctgatc
     3841 gaatggatgg agcgcgacga cgcccagcgc gtggcggcat gaccgggccg gcccccgcat
     3901 ctgtcattaa cgccctctcg gtcgatgtgg aggactggtt ccaggtcggc gccttcgagc
     3961 aggtgattga ccgggcggac tgggagagcc tggaatcgcg cgtggaaaac aattgcctgc
     4021 gcatcctcga cctgtttgcc gaatgcgggg tgaaggccac cttcttcacg ctgggctggg
     4081 tggcgcggcg ccagccggcg ctgatgcggc gcatcgtttc ggaagggcac gagctggcca
     4141 gccatggctg ggatcatgcc cgggtgttca cgctggatgc cgcccgcttt gccgaggatc
     4201 tgcgccgcgc ccgccaggcg ctggaagatg ccgccggggt ggcggtgatc ggctatcgcg
     4261 cgcccagctt ctcgatcgat gcgcgcacgc cctgggcgca tccggtgctg gccgagcagg
     4321 gctatgccta ttcctccagc gtctcgccca tcgcgcatga tcattatggc tggcgcgcgg
     4381 cgccgcgttt tgccttccag ccggtggcgg gctcggccct gctcgaagtg ccggtcacca
     4441 gcgtggagct gggcgggcgg cgctttgccg caggcggcgg cggcttcttc cgggtgctgc
     4501 cctatggttt cagccgctgg gcgatccggc gggtgaacgg ccatgatggc ctgcccgcca
     4561 tcacctattt ccacccctgg gagatcgacc ccggccagcc acgcgtggcg ggggcaccct
     4621 tgcgctcgcg cctgcgccat tacaccggcc ttggcgcgat ggaaggcaag ctgcgccgcc
     4681 tgctgggcga tttccgctgg gggcggatgg acgaattgtt ctgcccggtg gcgggcgagg
     4741 atgtgagcgc ggcggcatga acgcgccctg gctttccacg cgggggggcc gcgcgcggct
     4801 ggtcgatccg gccggggctg aaggcgcccg gctggaggct ttcgtggcca gccatccgga
     4861 gggcacgctg tttcaccggc ccgcctggct gtgcgcggtg gcgcgcgggg cggggcaccg
     4921 ggcggcgatg ctggtgctgg aaggcgcgca ggagatcgcc gcgctgctgc cggtcaacct
     4981 catccattcg ccgatcttcg gccgggtgat cgcttccagc ggctttggcg tgggcggcgg
     5041 cctgctggcg ctgccggggg tggatgtggc gccgctggtg gcagcggtgg aggaactggc
     5101 ggcgcgctgg tcgtgcccgg gggtggaact gcgcggcggc ccgctgcccg aggggcggcc
     5161 gggctggcgc atccgcgccg atgcccatgc cggcttcatc cgcccgctgg ccgccgatga
     5221 cgaggccgag ttgctggcca tcccccgcaa gcagcgcgcc gaggtgcgca agggccttgc
     5281 cgctgatctt gccatcacca tcggcagcag cgcggcggac cgggcggcgc attatgccgt
     5341 ctatgccgaa agcgtgcgca atctgggcac gccggtgttc ccgcgcgcgc tgtttgccgc
     5401 catgctcgat ggctttggtg cggatgccga tatcctcacc gtcagccatc agggggtgcc
     5461 ggtggccagc gtgctgtcct tctatcacgc gggggccgtg ctgccctatt ggggcggcgg
     5521 cacgctggcg gcgcggggcc tgcgcgccaa tgatcgcatg tatttcgagc tgatgcgcca
     5581 tgcccgcgcg cgcggctgca cccgtttcga tttcggccgc tcgaagaccg gcagcggcgc
     5641 ttttgccttc aagcgcaact gggggtttga tccggcgccg ctggcctatg ccagctggca
     5701 ggcacccggc cagccgctgc gcgatgccga tcccaccagc gccgtccatg cccggcggat
     5761 agacctgtgg aagcggctgc cgctgccgct ggccaatctg ctggggccct tcatcgcgcg
     5821 ggggctggga tgatctcggg cgagatcctg atgctggcgc accgcattcc cttcccgccc
     5881 gaccggggcg acaagatccg ctcgcatcat atcctgaaga agctggtgcg catggcgccg
     5941 gtgcatgtgg cctgctttgc cgataccgat gctgacctcg ccgaggaagt ggagctggcc
     6001 gcggttgctc acagctatcg gctgatccgg cgggccaaat cgctgttcac ggcgggcgcg
     6061 caggcgctgc tgatgcatcg gccggtcagc ctcacggcgt ttcacgatcc gggtttggcc
     6121 gattatgtaa agcgcctgct cgccaccggg cgggtgcgca cgatctacgt tttttccggc
     6181 cagatggcgc aatatgtgcc ggcggatttc accgggcggc tggtgatcga tttcgtcgat
     6241 gtcgattcgg ccaagttcga ggcctatgcc gcccgccacc gggggccgat gggctggctc
     6301 tatgcccgcg aggccaggct gctgagcgcc gaggaacagc gccaggccgc ccgggccgat
     6361 gcggtgctgc tggtcagcca tgccgaggcg gatctgtttg ccagccgcat gcccccgggc
     6421 gctgccaccc aggtgcgcgt gctgggcaat ggcgtggatg ccgatgccta tgatccggcc
     6481 cagcaattcg ccgcaacgcc gatggccggc catggcgcgc cccggctgat cttcaccggg
     6541 cagatggatt atccgcccaa catcgaggcg gccatgcgca tggcccggcg gatcatgccg
     6601 ctggtgcgcg cgcaatgccc ggaggccagt ttccatgtgg tgggccggca ggcgggcgag
     6661 aaggtgctgg cgctggatgg cctgcatggc accaaggtgt ggggtgaggt ggccgatatc
     6721 cgcccctggc tgcgcgcggc ggatatggcg gtggtgccgc tggatattgc ccgcggcgtg
     6781 cagaacaagg tgctggaagc catggcgatg ggcctgccgg tggtggccag ccatgccgcc
     6841 gccacgggga tcaacgccat tgccggccat cacttcatgg tggccgataa tgacacggcg
     6901 attgccgccg cggtagtgag cctggcgcat gatccgcatc tgatgttccg catggggcag
     6961 gaagggcgcc gcttcgtgat cgagcatcag ggctggcaaa gggtgctggc gccgctggcc
     7021 ggcctgctgt cctctgcccc gccgcccccg cgtgaaccgc gagcgggcgc atgatcgcaa
     7081 aagaggcggc cggccgcgtg cctgcgcaag cggccggtgt ggattggcgc atggcgctgg
     7141 gcctgtgggc ggcagcggcc ggcgcgctgg tgatgctggc gcgggccgaa tggctggcga
     7201 tggccggcca gtggctgggc agttccacct acgagcatat cctgctggtg ccggcgatca
     7261 tcctctggct ggtggccgag cggcggccca gcct
//
LOCUS       DASRYE010000117        14361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_112680,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000117 DASRYE010000000
VERSION     DASRYE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112680"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFF98_06455"
     CDS             <1..586
                     /locus_tag="VFF98_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU63304.1"
                     /translation="FELWLRQCRAMIARMPGERIGIIGHSISGALALRLAASEPRVAG
                     VLATGTMGAPFALTEGTRLCWTYPETRADLLRAARALIADEALIDEAYVAAREAVLRQ
                     SGYGAYFNTMFGGDKQAYIDAAVIPPAELARITCPVTLLHGRADGGFPAEPLSLALAR
                     HLPQADVVLLGGCSHSIAMERPGHLLDAARRLFG"
     gene            627..1490
                     /locus_tag="VFF98_06460"
     CDS             627..1490
                     /locus_tag="VFF98_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU63305.1"
                     /translation="MKFVSYLAGDAACLGVLEGAAIVPVPGTMNAVIAGGAGALAAIA
                     AHVAASDERLPLADTPLLAPIPAPLRNIFCVGKNYSEHAREFHDSGFDASAGASAEPD
                     HPIIFTKANTTVAAPGAVIPAHLDPTASVDYEGELAVVIGKGGRGIGKAEAMDHVFGY
                     TIINDVTSRTLQNRHRQWFIGKNIDGFGPMGPTLVTADEVPDPAALRLLTHVNGEKRQ
                     EALVAQLIFDIPTLIETLSATMTLQPGDIIATGTCAGVGIGFDPPRFLQAGDVVAVTI
                     APIGTLENPVA"
     gene            1494..2786
                     /gene="gluP"
                     /locus_tag="VFF98_06465"
     CDS             1494..2786
                     /gene="gluP"
                     /locus_tag="VFF98_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HZU63306.1"
                     /translation="MGPQQAPGGADVAGIGGGAVLPLRLLAGMITGIYFFGGILTAMV
                     DILVPKFKALFTLNYTEAMLTQFCYFLAYLVFSLPAGRIAARLGAVTGTALGVAIMAL
                     GCVLFAPAAWLGSYPAFLFALFVVSAGTNLFQISANPLIARLGGAGGAHGRLALGLAF
                     NALGYAVGPLVGTALILSASAMALPDPAHAPPAVLAAYRLAEGQIIQRPFLGLALGLV
                     VLAGAVLAVGARALRAGGAMLAPVAWRWGVWRNRRLVAGALAIFCYVGAEVSIGSLLI
                     SYLGLPRVLALPMGEAGHFVALYWLGAMAGRFVGSFTLGRVPAGRQLAGHGLAAIGLI
                     GLSLASSGWLAAASLIGVGLANSIMFPALFTLSLEELGEEAPIGAALLCMAIVGGAIL
                     PLVTGGLADLVGLGWAPVVPALCYGAVVSFGLAARPAA"
     gene            2971..4071
                     /locus_tag="VFF98_06470"
     CDS             2971..4071
                     /locus_tag="VFF98_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU63307.1"
                     /translation="MKFSVFLNARSMAPEADRQLMLDLESHALLAADLGYEAIFLPDH
                     HFNGYMPVASDSFIFAAYLAAKMPQMHFGFSVVSVPLHHPVRFAERINVLDQLTQGKL
                     LVGVGSGTTPEEMIGFAVNYKDAGAISDRNLEIAEQLWAKRMDDPAVEIANGPYNGRV
                     LQRITPAPYSPGHARLMPVALKEASARRAAKHGWPAFIPAFTPPAIYGTEPFDHVKKW
                     FEIYKGYLLAEGHADTVVADALGWTTHTYQCVHIAESEDQAREELHEILTAYQEAVDR
                     ENAFNARAESDAANRKSDVNIRATDEGWIGTWCLWGTPESVSEHLRAYQDLGIGNILC
                     GTTTGPLTQRRLDLGNQTLRLLSEQVMPALKG"
     gene            4172..6535
                     /locus_tag="VFF98_06475"
     CDS             4172..6535
                     /locus_tag="VFF98_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU63308.1"
                     /translation="MREPTIEETCALTAGAAMWASLAVPEAGIRAFTMSDGPMGIASG
                     KVDERDVSLLTPCPAALGASWDVDLCARVGAVVGSDAAARGVDAVLAPNLNLARSPLA
                     GRAFEYFSEDPVLAGLLGAGWIAGLQSTGTAAVAKHLVCNDSETERDRVDVQVDEASL
                     REVYLRPFQIAVRAGCAGLLAAYNKVNGSWCSEQGRVLTDIVKGEWGFAGAIMSDWFG
                     THSSAGAINAGLDLEMPGPARFMGGKLAPLVADGTVGLARVADAAARVAATARAATGP
                     KAPPLAPGAAQAVLEEAAAAGMVLVKNAGGLLPLAPAGLKRLAVIGPNAAAPCYQGGT
                     FAKIAVAPGTPTPIDSIRARFGGQCEVVYEAGVDPQPRLPAMPVVPALDIGDGCTAGM
                     SLEYFVDPGAAPLFRETRATNSLVWFVGVHEEGGFDQPGMVRASGLFRAEQAGAHLFY
                     LGSTGAARLLVEGAEVLATGGEVPASDVMGKLKAGDADHVAVMLAAGQQLRITVEFTH
                     GAARVHGLWYGIRRPDSAEAMFARAVEAAREADAVVLVVGETSDSSVESKDRPDTRLP
                     AEQIRLIEAVCAANPRVAVVSNVGHAFDCGWDGLAAAHLVAWYPGQGFGPALAAVLAG
                     DAEPAGRLPLTIAEAEADYPALGLAPDAAGHLAYAEGLLVGYRGLIARGRKARHPLGS
                     GLGYARVDWLEARAEAGAVAVRLRNATARPASEVVQLYADAPHCALVGFAKAQLAPGE
                     EIWLRVPVEPLALERWVAGGWQRLAAPLALRAARHAEDPGLAVVLPG"
     gene            6629..7657
                     /locus_tag="VFF98_06480"
     CDS             6629..7657
                     /locus_tag="VFF98_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU63309.1"
                     /translation="MRLPRRLLAILSASGLALAGGVAAASPAAPPELEENRPWGQVAL
                     TFDDLPGLTLFADQPYVDYFNEMLVRGLKRHHLPAIGFVNESKLDEIQRERQIANLRR
                     WLNAGENLGNHTFSHESPNDLGAAGYIADIARGEPVTRALLAEKHKPLGWFRHPYLET
                     GFPAAAKKQIDDWLAAHGYRVAPVTIDADDWEFAEPYDDAISRHDAARVARIRRQYLD
                     YTERTIAWYQRASQEVFGRQIAFVMLLHDTRLNADSIDDLAAILQRRKLKGVPLEKAM
                     KDEAYRLGDPYVGKDGIDWIERWADKLHRNEPWASWRDVPKQIEDEYERNNHDRHEPP
                     APAVPTVK"
     gene            complement(7661..8548)
                     /locus_tag="VFF98_06485"
     CDS             complement(7661..8548)
                     /locus_tag="VFF98_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63310.1"
                     /translation="MTLDQLRIFIAVAEALNMTRAAETLHLTQPSVSAAVAALEGRHG
                     LLLFNRVGRGLELSEAGRAFLPEARAVLARAAEAAGTLDDLAGLKRGQLRLAASQTVA
                     TYWLPVRMARFAARWPGVALSLAVGNTQEAAALVLAGEADLGFVEGETEAPLLARRPI
                     GADRLGLYARPDHPLALHAEARRPLAPAELASAAWVLREAGSGTRAHFEDALAAAGLA
                     PAALDIRLELPSNGAVLEALAAGALVGAVSELAAGARVAAGQIVRLDWPLPARRFTLL
                     THRARRASRAVQAFQELLD"
     gene            8624..9643
                     /locus_tag="VFF98_06490"
     CDS             8624..9643
                     /locus_tag="VFF98_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZU63311.1"
                     /translation="MTQPLSPRRLLPGLALALVISALAYGVEWVEIRLWGRAYLEALV
                     VAILLGAVWRTVIGLPVRAASGIRFAAKPVMEGAVALMGASVSLAVLAHTGLALAGAI
                     AGTIALTILAGYRIGRWLGLPVRMALLVACGNAICGNSAIAAVAPAIDADGEDVAAAV
                     GFTAVMGIVVVLAVAPLAWALQLSPVQGGMLAGMTVYAVPQVLAAAGPLGAMAVQVGA
                     VVKLVRVLMLAPVVAVLSVLGGKAQAQEGMGAGGHLARLVPGFILVFLGLAGLRSLGL
                     VGDGLAALAHGLSMGGTVVAMAGLGLGVDLRQLRAAGPRVSAAVVLSLVVLFAGALAV
                     IHWLG"
     gene            complement(9650..11407)
                     /locus_tag="VFF98_06495"
     CDS             complement(9650..11407)
                     /locus_tag="VFF98_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZU63312.1"
                     /translation="MATRALRQTLRQSGINARRWLRGSEAVFILLAMLTGAAAGIATL
                     AQGWLAHELQHLLYGVTINRLSALGSIHHPWRLLALPLGGLVLVGLGRLQARRARPPI
                     DVVEANALHGGRVPPIDTALIAAQTLVSNGCGASVGLEATYAQMGGGIGSLLGQWLKL
                     RRADLRTLVGAGAGAGVGAAFGAPLAGAFYAFEIVIGTYSTAAVAPVIAATLAAVVLM
                     RQLGAEPYLIATTANRAITLVDYACYAGLGLLSALVGIAIMRLVTFAERGLQWATRAS
                     RWRPLLGGLLLMPIAWASPQTLSAGHGALHLDLVLHPPIAFLLVVLGLKIAASVISLA
                     SGYRGGLFFASLFLGSLLGQAYGEIVNLNPWGLAIDPNDAALVGMAGLAVSIVGGPMT
                     LALLMLETTHDFALMGVVLTAALLSSLVTREAFGYSFSTWRLHVRGSDIRSPRDIGWM
                     LSLNAGRMMRRDWVAVPALASVAQFRQAVPLGSASKAILTDEQGHYHGIVPTAAAYAP
                     ALDPQGAVAALARLADSTLNPFTGIKETLRAFDAAMADELAVVDPEGRVVGVLSEKHA
                     RRRYLEEIEAAQRQMFGEG"
     gene            complement(11412..12305)
                     /locus_tag="VFF98_06500"
     CDS             complement(11412..12305)
                     /locus_tag="VFF98_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HZU63313.1"
                     /translation="MILRPKPGLAEVLFAVRGTILPRIAGRMVLIAAVSVGAVLAAGA
                     YRGIFAQIGAIPLTLIGLALSIFMSFRNNACYDRWWEGRKQWGALVIAARSLARQAAL
                     LGPAEREGLLRGVAGFAGGLAARLRGRDEAAALAPWMPAPDPCAPNPTDAALRLLGAE
                     TQRLLAQGQIDGWRWHALEEQLGALSGVQAACERIAATPVPFAYSLLLHRTAHVFCLC
                     LPLALAGTMGWWTLLPALLVGYTFFGLDALGDQLEDPFGTEPNDLPIDAIVRTVEREM
                     LAALGVADLPPPLAPVGGVLL"
     gene            complement(12356..13666)
                     /locus_tag="VFF98_06505"
     CDS             complement(12356..13666)
                     /locus_tag="VFF98_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZU63314.1"
                     /translation="MRKSLAWPLALAGAAAPTLARAADAVNAAHQANGVDTIWILISS
                     TLVLLMCMPGLGLFYGGLVRTKNFLSVLVQVGAIAAVGSLLWIVCGYTIAFGTVTNGW
                     FGATNALMLAGLAPLRAGLALPENSFALFQMCFAAITPALMAGAWVDRARFGWVIGFC
                     ALWGLVVYAPAAHWIWGGGWLATRLGTQDFAGGIVVHTTAGVSALVVAAMLGKRQGFP
                     KTLMLPHSPALTMVGAALLWVGWFGFNGGSALAGNGTAVNAIINTHVAASAAAMVWLG
                     IERFVVGKPTSVGFATGAIAGLATVTPAAGFIAPGAALLLGCTGAAVCFPMIQLVKQK
                     WRIDDSLDVFAVHGVGGMTGSVLLALFLSPALGGAGYSQDMDLARQALAQLAGVGVVA
                     LWSAAASVAIALAVAQIVPMRVGEDDEREGLDLASHGERAWEMD"
     gene            complement(13886..13959)
                     /locus_tag="VFF98_06510"
     tRNA            complement(13886..13959)
                     /locus_tag="VFF98_06510"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13925..13927),aa:Cys,seq:gca)
     gene            14188..>14361
                     /locus_tag="VFF98_06515"
     CDS             14188..>14361
                     /locus_tag="VFF98_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HZU63315.1"
                     /translation="MKTRAAVAFAPKTPLEIVEVDLEGPKAGEVLVEIMATGVCHTDA
                     YTLDGFDSEGIFPS"
BASE COUNT         2131 a         4854 c         5198 g         2178 t
ORIGIN      
        1 tttcgagctg tggctgcgcc agtgccgggc gatgatcgcg cgcatgccgg gcgagcggat
       61 cggcatcatc ggccattcga tctcgggcgc gctggccttg cggctggccg ccagcgagcc
      121 gcgggtggcc ggcgtgctgg ccaccggcac gatgggggcg ccctttgccc tcaccgaagg
      181 cacgcgcctg tgctggactt accccgaaac gcgcgcggat ctgctgcgcg cggcgcgcgc
      241 cctcatcgcc gatgaggcgc tgatcgacga ggcttatgtc gccgcgcgcg aggcggtgct
      301 gcgccagagc ggctatggcg cctatttcaa cacgatgttc ggcggcgaca agcaggccta
      361 tatcgacgcg gcggtgatcc cgcccgccga gctggcccgc atcacctgcc ctgtcaccct
      421 gctgcacggg cgcgcggatg gcggctttcc cgccgaaccg ctgagcctgg cgctggcccg
      481 ccatctgccc caggccgatg tggtgctgct ggggggctgt tcgcattcca ttgccatgga
      541 acggcccggc cacctgcttg atgcggcccg gcgcctgttc ggttgaccaa gcgcctgcac
      601 aaaggcgcgc atacaggagt tctgccatga aattcgtcag ctatctggcg ggcgatgccg
      661 cttgcctggg tgttctggag ggcgcggcca tcgtgccggt gccgggcacg atgaacgcgg
      721 tgatcgccgg cggtgccggg gcgctggctg ccatcgccgc ccatgtggcc gccagcgatg
      781 aacgcctgcc cctggccgat acgccgctgc tggcccccat tccggcgccg ctgcgcaaca
      841 tcttctgcgt gggcaagaat tattccgaac atgcccgcga attccacgac agcggctttg
      901 atgccagcgc cggcgccagc gccgagcccg accatccgat tatcttcacc aaggccaaca
      961 ccacggtggc cgcgcccggc gctgtcattc ccgcccatct cgatcccacc gcctcggtgg
     1021 attacgaggg cgagctggcg gtggtgatcg gcaagggcgg ccgcggcatc ggcaaggccg
     1081 aggcgatgga ccatgtgttc ggctacacga tcatcaacga tgtcacctcg cgcacgctgc
     1141 agaaccgcca ccgccagtgg ttcatcggca agaacatcga tggtttcggc cccatgggcc
     1201 ccacgctggt cactgccgat gaagtgcccg acccggccgc gctgcgcctg ctcacccatg
     1261 tgaacggcga gaagcggcag gaggcgctgg tggcccagct gatcttcgac ataccgacgc
     1321 tgatcgaaac cctttcggcc accatgaccc tgcagccggg cgacatcatc gctactggca
     1381 cttgcgccgg ggtgggcatc ggtttcgatc cgccgcgctt cctgcaggcg ggcgatgtgg
     1441 tggcggtgac gatcgcgccg atcggcacgc ttgaaaaccc ggtggcctga gcggtggggc
     1501 cgcagcaggc gccgggcggg gcggatgtcg ccgggatcgg gggcggcgcc gtgctgccgc
     1561 tgcgcctgct ggcggggatg atcaccggca tctatttctt cggcggcatc ctcaccgcca
     1621 tggtggatat actggtgccc aagttcaagg cgctgttcac cctgaactac accgaggcga
     1681 tgctgacgca gttctgctat ttcctcgcct atctggtgtt ctcgctgccg gccgggcgga
     1741 tcgcggcgcg gctgggcgcg gtaacgggca cggcgctggg ggtggcgatc atggcgctgg
     1801 gctgcgtgct gtttgcgccg gcggcctggc tgggcagcta tccggccttt ctcttcgcgc
     1861 tgttcgtggt gtcggccggc accaacctgt tccagatctc ggccaatccg ctgattgccc
     1921 ggctgggcgg cgctggcggc gcgcatgggc ggctggcgct ggggctggcg ttcaacgcgc
     1981 tgggctatgc ggtggggccg ctggtgggca cggcgctgat cctgtcggcc tcggcgatgg
     2041 cgctgcctga tccggcccat gcgccgccgg cggtgctggc ggcctatcgg ctggccgagg
     2101 ggcagatcat ccagcgcccc ttcctggggt tggcgctggg gctggtggtg ctggcgggcg
     2161 cggtgctggc ggtgggcgcg cgcgcgctca gggctggcgg ggcgatgctg gcgccggtgg
     2221 cctggcgctg gggcgtgtgg cgcaaccggc ggctggtggc aggggcgctg gccattttct
     2281 gctatgtggg tgccgaagtg tcgatcggca gcctgctgat cagctatctg ggcctgccgc
     2341 gcgtgctggc cctgccgatg ggcgaggcgg gccatttcgt cgcgctctac tggctggggg
     2401 cgatggcggg gcggtttgtc ggcagtttca cgctggggcg ggtgccggcg gggcggcagc
     2461 tggcgggcca tgggctggcg gcgatcggcc tgatcggcct gtcgctggcg agcagcggct
     2521 ggctggcggc ggccagcctg atcggcgtgg ggctggccaa ttcgatcatg ttcccggcgc
     2581 tgttcaccct gtcgctggag gagctgggcg aggaagcgcc gattggcgcg gcgctgttgt
     2641 gcatggcgat tgtgggcggg gcgatcctgc cgctggtcac cggcggcctg gctgatctgg
     2701 tcgggctggg ctgggcgccg gtggtgccgg cgctgtgcta tggcgcggtg gtgagctttg
     2761 gcctggcggc gcggcccgcc gcctagccgt ttcctgaagc atctgcgggc agggccccgc
     2821 gcctggcatg ggccgggcgc ggggcttttg cgtgcgcggg gcggttgaca gggttgggcg
     2881 cttcccgtta atgaaccaaa acaatcattc taattaatga ccggcccaag cgccggcctc
     2941 aatcacgcgg atcgcgccgg ggagagaaaa atgaagtttt ccgtgttcct caatgcccgt
     3001 tcgatggcac ccgaggctga tcgccagctc atgctcgatc tggaatcgca tgcgctgctg
     3061 gcggcagacc ttggttatga ggcgatcttc ctgcccgatc accacttcaa cggctatatg
     3121 ccggtggcgt ctgacagttt catcttcgcg gcctatctgg cggccaagat gccgcagatg
     3181 catttcggct tctcggtcgt ctcggtgccg ctgcaccatc cggtgcgctt tgccgagcgg
     3241 atcaacgtgc tcgaccagct gacgcagggc aagctgctgg tgggcgtggg cagcggcacc
     3301 acgcccgagg agatgatcgg ctttgccgtg aactacaagg atgccggcgc catttccgat
     3361 cgcaatctgg aaatcgccga gcagctgtgg gccaagcgca tggacgatcc cgccgtggaa
     3421 atcgccaatg gcccctataa cggccgcgtg ctgcagcgca tcaccccggc gccctattcg
     3481 cccggccatg cccggctgat gccggtggcc ctgaaggagg cttcggcccg gcgcgcggcc
     3541 aagcatggct ggccggcctt catccccgcc ttcacgccgc ctgccatcta tggcaccgag
     3601 ccgttcgacc atgtgaagaa gtggttcgag atctacaagg gctacctcct ggccgaaggg
     3661 catgccgata ccgtggtggc cgatgcgctg ggctggacga cgcacaccta tcaatgcgtc
     3721 cacattgccg agagcgagga tcaggcgcgc gaggagctgc acgagatcct gacggcctat
     3781 caggaagcgg tggaccgcga gaacgccttc aacgcccgcg ccgaaagcga tgcggccaac
     3841 cggaagagcg atgtgaacat ccgcgccacc gacgagggct ggatcggcac ctggtgcctg
     3901 tggggcacgc ccgaaagcgt gagcgagcac ctgcgcgcct atcaggatct gggcattggc
     3961 aatatcctgt gcggcaccac caccgggccg ctcacccagc gccggctgga tctgggcaac
     4021 cagacgctgc ggctgctgtc ggaacaggtg atgccggcgc tgaagggctg aagggctgaa
     4081 gggctgaggg gcgcggcgcg cgggggctgc cccttcgcgc gcccagccac cctgccggcc
     4141 ggacaacaga gaaaagatcg ggagccaagc gatgcgagag ccaaccatcg aggaaacctg
     4201 cgcgctgacc gcgggcgcgg ccatgtgggc cagcctggcg gtgccggaag cgggcattcg
     4261 cgccttcacc atgagcgacg ggccaatggg catcgccagc ggcaaggtgg acgagcgcga
     4321 cgtgtcgctg ctgacgcctt gcccggcagc gctgggggcc agctgggatg tggatctgtg
     4381 cgcacgggtg ggcgcggtgg tgggcagcga tgcggccgcg cgcggcgtgg atgcggtgct
     4441 ggcgcccaac ctcaatctgg cgcgcagccc gctggcgggc cgggccttcg aatatttctc
     4501 ggaggatccg gtgctggcgg gcctgctcgg ggcgggctgg atcgcgggcc tgcaatccac
     4561 cggcaccgcc gccgtggcca agcatcttgt ctgcaacgac agcgagacag agcgcgaccg
     4621 ggtcgatgtg caggtggatg aggcgagcct gcgcgaggtc tatctgcggc ccttccagat
     4681 cgccgtcagg gcgggctgcg cggggctgct ggcggcctat aacaaggtca acggcagctg
     4741 gtgctccgaa caggggcggg tgcttaccga tatcgtcaag ggcgagtggg gctttgccgg
     4801 cgccatcatg agcgactggt tcggcaccca cagcagcgct ggcgcgatca acgccgggct
     4861 ggatctggaa atgccgggcc cggcccgctt catgggcggc aagctggcgc cgctggtggc
     4921 cgatggcacg gtgggcctgg cgcgcgtggc cgatgccgcg gcccgcgtgg cggccaccgc
     4981 gcgggcggcc accggcccca aggcgccgcc actggccccg ggcgcagcgc aggcggtgct
     5041 ggaggaagcg gcggcggctg gcatggtgct ggtgaagaat gccggcggcc tgttgccgct
     5101 ggcgccggcg gggctgaagc ggctggcggt gatcggcccc aatgcggcgg cgccttgcta
     5161 tcagggcggc acctttgcca agattgccgt ggcgccgggc acgcccacgc cgatcgacag
     5221 catccgcgcg cgctttggcg gccagtgcga ggtggtctac gaggctggcg tcgatccgca
     5281 gccgcgcttg ccggcgatgc cggtggtgcc ggcgctggat atcggcgatg gctgcactgc
     5341 gggcatgagc ctggaatatt tcgtcgatcc tggcgctgcg ccgctgttcc gcgagacgcg
     5401 ggcgaccaat tcgctggtgt ggtttgtcgg cgtgcatgag gagggcgggt tcgaccagcc
     5461 gggcatggtg cgcgccagcg ggctgttccg cgccgaacag gccggcgccc acctgttcta
     5521 cctcggttcc accggcgcgg cgcggctgct ggtggagggc gccgaagtgc tggccaccgg
     5581 cggcgaggtg cccgccagcg atgtgatggg caagctgaag gcgggcgatg ccgatcatgt
     5641 ggcggtgatg ctggcggccg gccagcagct gcgcataacg gtggagttca cccatggcgc
     5701 ggcgcgggtg catggcctgt ggtatggcat ccgccggccc gacagcgccg aggcgatgtt
     5761 cgcccgcgcg gtggaggcgg caagggaggc cgatgcggtg gtgctggtgg tgggcgaaac
     5821 ctccgattcc agcgtggaat cgaaggatcg gcccgacacg cggctgcccg ccgagcagat
     5881 ccgcctgatc gaggcggtgt gcgcggccaa tccgcgcgtg gcggtggtct ccaacgtggg
     5941 ccatgccttc gattgcgggt gggatgggct ggccgccgcc catctggtcg cctggtatcc
     6001 ggggcagggc tttggcccgg cgctggccgc cgtgctggcc ggcgatgccg agcctgccgg
     6061 ccgcctgccg ctgaccattg ccgaggccga ggcggattat cccgcccttg gcctggcgcc
     6121 cgatgcggcc ggccatctgg cctatgccga agggctgctg gtgggctatc gcgggctgat
     6181 cgcgcgcggg cggaaggcgc gccatccgct gggcagcggc cttggctatg cccgggtgga
     6241 ctggctggag gcgcgggccg aggcaggtgc ggtggcggtg cggctgcgca atgccaccgc
     6301 ccggccggcc agcgaggtgg tgcagctgta tgccgatgcg ccccattgcg cgctggtggg
     6361 ctttgccaag gcgcagctgg ccccgggcga ggagatctgg ctgcgcgtgc cggtggagcc
     6421 gctggcgctg gagcgctggg tggcgggcgg ctggcagcgg ctggccgcac cgctggcctt
     6481 gcgtgcggcg cgccatgccg aagatcccgg gctggcggtg gtgttgccgg gctgaggggg
     6541 cttgcaagcc gggcggcggg ccctaaggcg aggcgcaagg aggcccggtg ttgacccgtg
     6601 cctcgcctcg aaaggagccc ttgcccccat gcgcctgccg cgccgcctgc ttgccatcct
     6661 gtccgcctct ggtctggctc ttgctggcgg ggttgccgct gccagcccgg cggcgccccc
     6721 ggagctggag gagaaccgcc cctggggcca ggtggcgctg actttcgacg atctgcccgg
     6781 cctcaccctg tttgccgatc agccctatgt cgattatttc aacgagatgc tggtgcgcgg
     6841 gctgaagcgc caccatctgc ccgccatcgg tttcgtcaac gaaagcaagc tcgacgagat
     6901 ccagcgcgag cggcagatcg ccaatctgcg ccgctggctg aatgccggcg agaacctggg
     6961 caaccacacc ttcagccatg aatcgcccaa tgatctgggg gcggccggct atatcgccga
     7021 tatcgctagg ggtgagccgg tgacgcgcgc gctgctggcc gaaaagcaca agccgctggg
     7081 ctggtttcgc cacccctatc tggaaaccgg ctttcccgcc gccgccaaga agcagatcga
     7141 cgactggctg gccgcccatg gctatcgcgt ggcgccggtc accatcgatg ccgacgactg
     7201 ggaatttgcc gaaccctatg acgatgccat ctcgcgccac gatgccgcgc gggtggcccg
     7261 catccgccgg cagtatctgg actataccga gcgcaccatc gcctggtatc agcgcgcctc
     7321 gcaggaggtg ttcggccggc agatcgcctt tgtcatgctg ctgcacgaca cccgcctcaa
     7381 tgccgacagc atcgatgatc tggccgccat tctccagcgc cgcaagctga agggggtgcc
     7441 gctggaaaag gcgatgaagg acgaggccta tcgcctgggc gacccctatg tgggcaagga
     7501 tggcatcgac tggatcgagc gctgggccga caagctgcac cgcaacgagc cctgggccag
     7561 ctggcgcgac gtgcccaagc agatcgagga cgaatacgag cgcaacaacc acgaccgcca
     7621 cgagcccccg gcgccggccg tgcccacggt gaagtaaggc ctagtccagc agttcctgaa
     7681 aggcctgcac ggccctgctg gcccgccgcg cgcgatgggt cagcagggtg aagcgacggg
     7741 cgggcaaggg ccagtccagc cggacgatct gcccggccgc cacccgcgcg cccgccgcca
     7801 gttccgacac ggcgcccacc agcgcgccgg ccgccagcgc ttccagcacg gcgccattcg
     7861 atggcagctc cagccggatg tcgagtgcgg ccggggcaag gcctgcggcg gccagcgcat
     7921 cctcgaaatg ggcgcgggtg cctgatcctg cctcgcgcag cacccaggcc gcgctggcca
     7981 gctctgccgg ggccagcggc cggcgggcct cggcatggag ggccagcggg tgatcgggcc
     8041 gggcatagag gcccagccgg tctgcgccga tgggacggcg ggccagcagg ggcgcctccg
     8101 tctcgccctc gacaaagccc agatccgcct cgcccgccag caccagggcg gctgcctcct
     8161 gcgtgttgcc caccgccagc gacagcgcca cccccggcca gcgcgcggca aagcgggcca
     8221 tgcgcacagg cagccagtag gtcgccaccg tctggctggc tgccaggcgc agctgccccc
     8281 gcttcagccc ggccagatcg tcgagcgtgc cggccgcctc tgccgcgcgg gccagcaccg
     8341 cgcgcgcctc gggcaggaag gcccgccccg cttccgatag ttccagcccg cgccccaccc
     8401 ggttgaacag cagcaggccg tggcgccctt ccagcgcggc caccgcggcg ctgaccgagg
     8461 gctgggtgag atgcagcgtt tctgccgcgc gggtcatgtt cagcgcttcg gcaacggcga
     8521 tgaagatgcg caactggtcg agcgtcattc aatagaaata ctctatcgaa tatccacaat
     8581 caatcgattg gattttgcgc cgcataaagc ccagcttgcc ggcatgacac agccgctttc
     8641 ccctcgtcgt ttgctccctg gccttgcgct ggcgctggtc atcagcgcgc tggcctatgg
     8701 cgtggaatgg gtggagatca ggctttgggg ccgggcctat ctggaagcgc tggtggtggc
     8761 gatcctgctg ggcgctgtgt ggcgcacggt gatcggcctg ccggtgcggg cggcctctgg
     8821 catccgcttt gccgccaagc cggtgatgga gggggccgtg gcgctgatgg gcgcgtcggt
     8881 tagccttgcc gtgctggcgc ataccgggct ggcgctggcc ggcgccattg ccggcaccat
     8941 cgcgctgacg atcctggccg gctatcgcat tggccggtgg ttggggctgc cggtgcgcat
     9001 ggcgctgctg gtggcttgcg gcaatgccat ctgcggcaat tcggcgatcg ccgcggtggc
     9061 gccggccatc gatgccgatg gcgaggatgt ggctgccgct gtgggcttca ccgcggtgat
     9121 gggcattgtt gtggtgctgg cggtggcgcc gctggcctgg gcgctgcagc tcagcccggt
     9181 gcagggcggc atgttggcag ggatgaccgt ttatgccgtg ccgcaggtgc tggccgcggc
     9241 cgggccgctg ggcgccatgg cggtgcaggt gggcgcggtg gtgaagctgg tgcgggtgtt
     9301 gatgctggcg ccggtggtgg cggtgctgtc ggtgctgggc ggcaaggcgc aggcgcagga
     9361 aggcatgggc gctggcgggc atctggcgcg gctggtgccg ggcttcatac tggtgtttct
     9421 ggggctggcg gggctgcgct cgctggggct ggtgggcgat gggctggcag cgctggccca
     9481 tgggctcagc atgggcggca cggtggtggc gatggccggg ctggggctgg gggtggatct
     9541 gcgccagctg cgcgcggcag gcccccgggt cagcgcggcg gtggtgctgt cgctggtggt
     9601 gctgtttgcc ggggcgctgg cggtgatcca ctggctgggg tgaggggaat tacccttccc
     9661 cgaacatctg ccgctgcgcc gcctcgatct cttccagata gcggcggcgg gcgtgtttct
     9721 cgctcagcac gcccaccacc cgcccttcgg gatcgaccac ggccagctcg tcggccatgg
     9781 cggcatcgaa ggcgcgcagg gtttccttga tgccggtgaa ggggttgagc gtgctgtcgg
     9841 ccagccgggc cagcgccgcc accgcccctt gcggatcgag cgcgggggca taggcggccg
     9901 ctgtcggcac gatgccatgg taatgcccct gctcatcggt gaggatcgcc ttgctggccg
     9961 agcctagcgg caccgcctgg cgaaattgcg ccaccgaggc aagggccggc accgccaccc
    10021 agtcgcgccg catcatccgc ccggcgttga gcgagagcat ccagccgata tcgcgcggcg
    10081 agcggatatc cgatccgcgc acatgcagcc gccaggtgga gaaggaatag ccaaaggcct
    10141 cacgcgtgac caggctggag agcagcgcgg cggtcagcac cacgcccatc agcgcgaaat
    10201 catgcgtcgt ctccagcatc agcagcgcca gcgtcatcgg cccgccgacg atcgacaccg
    10261 ccagcccggc catgcccacc agcgccgcgt cattggggtc tatcgccagg ccccaggggt
    10321 tgaggttgac gatctcgcca taggcctggc ccagcagcga gcccagaaac agcgaggcaa
    10381 agaacagccc cccgcgatag ccgctggcca gcgagatgac cgaagcggcg atcttcagcc
    10441 cgagcaccac cagcaggaag gcaatcggcg ggtgcagcac cagatcgagg tgcagcgcgc
    10501 catgcccggc cgacagcgtt tgcggcgagg cccaggcgat cggcatcagc agcagcccgc
    10561 ccagcagcgg ccgccagcgg ctggcgcgcg tggcccactg caggccccgc tcggcaaagg
    10621 tgaccaggcg catgatggca atgcccacca gcgccgacag cagccccagc ccggcatagc
    10681 aggcataatc caccagcgtg atcgcccggt tggcggtggt ggcgatgagg tagggttctg
    10741 cccccagctg gcgcatcagc accacggcgg ccagcgttgc cgcgatgacc ggcgccaccg
    10801 ccgccgtgct gtaggtgccg atgacgatct cgaaggcata gaaggcgccg gccagcggcg
    10861 cgccaaacgc cgcccccacg cccgcccctg cgcccgcgcc caccagcgtg cgcagatccg
    10921 cccgccgcag cttcagccac tggcccaaga gcgagccgat gccgccgccc atctgcgcat
    10981 aggtcgcctc cagcccgaca gaggcgccgc agccgttcga caccagcgtt tgcgcggcga
    11041 tcagcgcggt gtcgatcggc ggcacgcggc cgccatgcag ggcattggcc tccaccacgt
    11101 cgatcggcgg gcgggcccgg cgcgcctgca gccggcccag ccccaccagc accagcccgc
    11161 ccagcggcag cgccagcagg cgccaggggt ggtggatgga gcccagcgcg ctcagccggt
    11221 tgatcgtcac gccatagagc aggtgttgca gttcgtgcgc cagccagccc tgggccagcg
    11281 tggcgatgcc ggccgcagcg ccggtcaaca tcgccagcag gatgaacacc gcctcgctgc
    11341 cgcgcagcca gcgccgggcg ttgatccctg actggcgcag cgtctggcga agcgcgcggg
    11401 tggccatcgg cctagagcag caccccgccc accggggcca gcggcggcgg cagatcggcc
    11461 acgcccagcg cggccagcat ctcgcgctcc accgtgcgca cgatggcgtc gatcggcagg
    11521 tcgttgggtt cggtgccgaa ggggtcttcc agctggtcgc ccagcgcatc gaggccgaag
    11581 aaggtgtagc ccaccagcag cgccggcagc agggtccacc agcccatcgt gccggccagc
    11641 gccagcggca ggcacaggca gaagacatgc gccgtgcggt gcagcagcag cgaataggca
    11701 aagggcaccg gcgttgcggc aatccgctcg caggcggcct gcacgccgga aagggcgccc
    11761 agctgctctt ccagcgcgtg ccagcgccag ccatcgatct gcccctgggc cagcaggcgc
    11821 tgggtttctg cccccagcag ccgcagcgcg gcatcggtgg ggttgggcgc gcagggatcg
    11881 ggcgcgggca tccagggggc gagggcggcg gcctcgtcgc gcccgcgcag gcgggcggcc
    11941 aggccgccgg caaaaccggc cacgccgcgc agcagcccct cgcgctctgc cgggcccagc
    12001 agggcggcct ggcgggcaag gctgcgcgcg gcgatcacca gcgcgcccca ctgcttgcgc
    12061 ccttcccacc agcgatcgta gcaggcgttg ttgcgaaagc tcatgaagat cgacagggca
    12121 aggccgatca gcgtgagcgg aatggcgccg atctgggcga aaatgccacg ataggcgccg
    12181 gccgccagca ccgcgcccac cgatacggcg gcgatcagca ccatgcgccc ggcgatgcgc
    12241 ggcaggatcg tgccgcgcac ggcaaacagc acttcagcca gaccgggttt ggggcgcagg
    12301 atcatgcccc agcgctagcg ccgggcgggc ccggcggcaa gctttggcag gcgtttcagt
    12361 ccatttccca ggcgcgttcg ccgtgcgagg caaggtccag gccctcgcgt tcgtcatcct
    12421 cgcccacgcg catcggcacg atctgggcca ccgccagcgc gatcgccacg ctggccgccg
    12481 ccgaccacag cgccaccacg cccacgccgg ccagctgcgc cagcgcctgc cgggccagat
    12541 ccatgtcctg tgaatagccg gccccgccca gcgccggcga gaggaacagc gccagcagca
    12601 cagagccggt catgccgccc acgccatgca cggcaaacac atcgagcgaa tcgtcgatcc
    12661 gccacttctg cttcaccagc tggatcatcg ggaaacagac cgccgcgccc gtgcagccca
    12721 gcagcagcgc cgcgcccgga gcaatgaagc cggccgccgg cgttaccgtg gccaggcccg
    12781 cgatggcgcc ggtggcaaag cccacgctgg tgggcttgcc caccacgaag cgctcgatcc
    12841 ccagccacac catcgccgcg gcagaggccg ccacatgggt gttgatgatg gcattcaccg
    12901 ccgtgccatt gcccgccagc gccgagccgc cgttgaagcc gaaccagccc acccacagca
    12961 gcgccgcgcc caccatcgtg agggccggcg aatggggcag catcagcgtt ttggggaagc
    13021 cctggcgctt gcccagcatg gccgccacca ccagcgccga aacgcccgcc gtggtgtgca
    13081 caacgatgcc gccggcaaaa tcctgcgtgc ccagccgggt ggccagccag ccgccgcccc
    13141 agatccagtg cgcggccggc gcatagacca ccagccccca cagcgcgcaa aagccgatca
    13201 cccagccaaa gcgcgcccgg tccacccagg cgccggccat cagcgccggg gtgatcgcgg
    13261 caaagcacat ctggaacagg gcaaagctgt tttccggcag cgcaagcccc gcgcgcaagg
    13321 gagcaaggcc cgccagcatc agcgcgttgg tggcgccgaa ccagccgttc gtcaccgtgc
    13381 cgaaagcgat cgtgtagccg cacacgatcc acagcagcga gcccaccgcc gcgatcgccc
    13441 ccacctgcac cagcaccgag aggaagttct tggtgcgcac cagcccgcca tagaacagcc
    13501 ccaggcccgg catgcacatc agcagcacca gcgtcgagga tataaggatc cagatcgtgt
    13561 cgacgccatt ggcctgatgg gcggcattca ccgcatctgc cgcgcgggcg agcgtgggcg
    13621 cggccgcgcc ggcaagcgcc agcggccacg caagagactt gcgcatgggg ttgctctcca
    13681 ttttcccggc agacaccgcc tgccgaactc ctcaacgcag gtctctaggc gatggcgcgc
    13741 aataggcaag gggagggggc ggcgatggcc cgggaaagca tcgcgataat attatttctt
    13801 ctatcagaaa atctgatcgg gccggcagcg gcgcgcaaag accccgcgcc agcgcgccgg
    13861 gctttgccag attttgccgg aaggatggag gcccgagccg gaatcgaacc ggcgtatacg
    13921 gatttgcagt ccgctgcgtc accactccgc catcgggcca tccggtggtg agagcggcag
    13981 cccctagccg agggcgccgc ccatggcaat gggctttatc gcaagggtgc gcgcaaatcg
    14041 gcggggcggg ggctggcggg gcgcctgccg ccctcttgcc aagcgcggcg cgccgccgca
    14101 tgcacgtcag gtatagcaat cggggcgcct gcgtgccgca gcctgccgcc cggattgcat
    14161 gtgcgccctg ttgaggagtt cctgccgatg aaaacccgcg ctgccgtggc ctttgcgccg
    14221 aagacccctc tggagatcgt ggaggtggat ctggaagggc cgaaggccgg cgaggttctg
    14281 gtggagatca tggcgacggg cgtgtgccac accgatgcct acacgctgga tggcttcgat
    14341 tcggaaggga ttttcccttc g
//
LOCUS       DASRYE010000118         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_6510,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000118 DASRYE010000000
VERSION     DASRYE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6510"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..377)
                     /locus_tag="VFF98_06520"
     CDS             complement(33..377)
                     /locus_tag="VFF98_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HZU63316.1"
                     /translation="MLALIQPGASFKDNLQRLPPIADVQRIDLVDAQGAVCATIENQP
                     GKQGSLAVYQYLRQAFGQLDAQAAAHGLAVFAEHTADARARPGAHPNIDRLLAIAAGA
                     APLAIEIIPASF"
     gene            complement(465..2585)
                     /locus_tag="VFF98_06525"
     CDS             complement(465..2585)
                     /locus_tag="VFF98_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU63317.1"
                     /translation="MINQQVNENLQNRANAEARFIKSELDTAFDAARNTANSFEQMAA
                     PGAGGTPAGSRRTQFTAVLRNILEQNPRFNGTYSAWEPNGLDGNDNAFRNLAAMGSDG
                     TGRFLPYWTRGTNGKIAIQPLVEYDSSARHPNGLVKGAWYINPHNTHHENLLGPLPYI
                     VQGKQVYLATMSVPIMINGVFHGVAGADYNLDFLQTLASQVSKSLYNGKSTVAVINDS
                     GLIVANSADPATIGGAAARANAAWANSANIIAEGKSVVLDDPKSPDLQIYSPIRIGRV
                     DTSWAVLISVPRAMVMADATLLSDQLGRRSGVDAMLQLLIGVGIAVAAIVMTWRMAGT
                     VSRPISRCAEFAEGIAQEKLNQQLAIAQDDEVGTLAEALRKMQKDLRTNLEQREHDQA
                     STQLVVDTVSKSLERLANGDLTSQITENFSAEYADVKRHFNAAIGSLHKIILSVEDSS
                     KTINIGAGEISQASVDLASRTETGAARLEQTASAMNSVTAIVRQTAENAADARRSIIS
                     TDQQAVEGGSIVSNAVMAMGEIEKSSEQISQIISLIDGIAFQTNLLALNAGVEAARAG
                     DAGKGFAVVASEVRSLAMRSAEAAQDIKKLINASSGQVAQGVALVGETGTALKHIIEQ
                     IGQLRETIDEIANSAEIQATDLVRINEAVGEMERMTQQNAAMVEESTAAARSLSDQAR
                     TLEAAVGQFNLNSHFHHARDRYAA"
     gene            2899..3096
                     /locus_tag="VFF98_06530"
     CDS             2899..3096
                     /locus_tag="VFF98_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63318.1"
                     /translation="MAEAGIGNAGIKAVTLHSRDDEVAIYVAAAEQRRLGKQSIARIA
                     KADVSNRKRERRLTRKENTAK"
     gene            complement(3215..3388)
                     /locus_tag="VFF98_06535"
     CDS             complement(3215..3388)
                     /locus_tag="VFF98_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HZU63319.1"
                     /translation="MSIKEIRAETSLHTATIYRRIKAGNFPAPRPIGGGRVAWLQSEI
                     EAWKQEILSNPPI"
     gene            complement(3522..4112)
                     /locus_tag="VFF98_06540"
     CDS             complement(3522..4112)
                     /locus_tag="VFF98_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HZU63320.1"
                     /translation="MSTPAPPPAAQALPIIRDWATQRGLSFLGGTKAGAVLSPCGRYR
                     YLLWREAGMAAPFMGFALLNPSTADATSNDATIRRCLSVMRRGIPGCGPLDGMLVWNL
                     FALRARDPAELLEAENPIGPHNDAAIALAMALAGGTIAGWGQQGHFFQRDLTVRLACA
                     KAGLQLHSLRRNRDGTPAHPLYLPGALIPMLWEFDW"
     gene            complement(4109..4369)
                     /locus_tag="VFF98_06545"
     CDS             complement(4109..4369)
                     /locus_tag="VFF98_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63321.1"
                     /translation="MSDPAFGAAFARQPDPTQETHYALSELAKYELDTLFAAMNTMGG
                     ILAEAPPDLDPLQVAPFFAAMGAYGARILADAPAIFPRRAAA"
     gene            complement(4636..5226)
                     /locus_tag="VFF98_06550"
     CDS             complement(4636..5226)
                     /locus_tag="VFF98_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZU63322.1"
                     /translation="MTHALVLTAADTAIRLAKVCPALCPPQQVTAATVESWLPIEGFP
                     EYAVSTEGRVYSHLSHRVLRQGRAHNTDLAGGQSYRSVVLRRNGRSFTKSVHRLVGLA
                     FLPNPTGEHLQIRHLNGDPSDNRMVNLAWGTASQNAMDRRAHGNETAAKITPEIAAEI
                     RQLCKLGADRQLLSQRYGVTTKHISRILRGDRWRIA"
     gene            complement(5223..5615)
                     /locus_tag="VFF98_06555"
     CDS             complement(5223..5615)
                     /locus_tag="VFF98_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63323.1"
                     /translation="MCAPCANADPLAVACPKCGAPAGMPCQRHSAALNAEHHVRSRRA
                     AFPPAARPAAASPGRRPACAGCAGLLAEALELSRRAKALDADVNRAVSASRGRCATPA
                     AAILTQYDQDLAAWEARSRQHLQQGCFA"
     gene            complement(5666..6370)
                     /locus_tag="VFF98_06560"
     CDS             complement(5666..6370)
                     /locus_tag="VFF98_06560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZU63324.1"
                     /translation="MPKPITFGPVTLWPGDAYAIRPRLGWMDADVLDPPYLIRATGAG
                     RYRKRRPNMDQLITDGLNRGFDMGIINPLLCGAAVVFAHNDQLAELLTRLQGGFHRQA
                     LCIWEKTNPQPIANRHYRADVEFYVHAWQRGWHPTGSIADRLRVRRLSSPRGADRHGH
                     PCPKPVPLMERIMRNVAGQSVCDPFMGVGTTGIAALRAGKRFVGIEHNPRHFAVAEAR
                     LRAELARLEAGVEIAA"
BASE COUNT         1110 a         1969 c         2334 g         1212 t
ORIGIN      
        1 gggggcgatg gccgccccgc cgattgagca gcctagaagc tggcggggat gatctcgatg
       61 gccagtggcg cagcgccggc cgcgatggcc agcaggcggt cgatattggg atgggcgccg
      121 gggcgggcgc gggcatctgc ggtgtgttcg gcaaacacgg ccaggccatg tgcggcggcc
      181 tgtgcatcca gctggccaaa ggcttgccgc agatactgat aaaccgccag cgatccctgc
      241 ttgcccggct ggttctcgat ggtggcgcac acggcgccct gcgcgtctac cagatcgatg
      301 cgctgcacat cggcgatggg cggcaggcgc tgcagattat ccttgaacga tgcaccgggt
      361 tgaatgagag ccagcatatt gcaaattcct gcgtggatgg gcgcgcgcct ctagcagctt
      421 gggccggcgc gcgccataag gcgcaggcgt tggaatgagc tgccttatgc cgcgtagcga
      481 tcgcgggcat ggtggaaatg gctgttcagg ttgaactggc caacggcggc ctccagcgtg
      541 cgggcctggt cgctcaggct gcgtgcggcg gcggtgcttt cctctaccat ggcggcattt
      601 tgctgggtca tgcgctccat ttcgcccacc gcctcgttga tgcgcacaag gtcggtcgcc
      661 tggatctcgg ccgagttcgc gatctcgtcg atggtctcgc gcagctggcc gatctgctcg
      721 atgatatgtt tcagcgcggt gccggtttcg cccaccagcg ccaccccttg cgccacctgc
      781 ccggaactgg cgttgatcag cttcttgatg tcctgcgcgg cctcggcgct gcgcatggcc
      841 agcgagcgca cctcgctggc caccaccgca aagcccttgc cggcatcgcc cgcgcgtgcg
      901 gcttccaccc cggcgttcag ggccagcagg ttggtctgga aggcgatgcc atcgatcagc
      961 gagatgatct ggctgatctg ctcggaggat ttctcgattt cgcccatcgc catcaccgca
     1021 ttgctcacga tcgagccgcc ctcgaccgcc tgctgatcgg tggagatgat ggagcggcgc
     1081 gcatcggcgg cgttctcggc ggtctggcgc acgatggcgg tcaccgaatt catggccgag
     1141 gcggtctgct ccaggcgggc agcgccggtt tcggtgcggc tggccagatc aaccgaggcc
     1201 tggctgatct cgccggcgcc gatgttgatc gtcttgctgc tgtcctccac cgacaggatg
     1261 atcttgtgca gcgagccgat cgccgcattg aaatgccgct tcacatcggc atattcggcc
     1321 gagaaattct cggtgatctg cgaggtcagg tcgccgttgg ccaggcgctc aaggcttttg
     1381 gacacggtgt cgaccaccag ctgggtgctg gcctggtcgt gttcgcgctg ctccaggttg
     1441 gtgcgcaggt ccttctgcat cttgcgcagg gcctccgcca gcgtgccaac ctcgtcatcc
     1501 tgcgcgatgg ccagctgctg gttgagcttt tcctgcgcga tgccttcggc aaattcggcg
     1561 cagcggctga tggggcggga taccgtgccg gccatccgcc aggtcatgac gatggccgcc
     1621 accgcgatcc ccacgccgat cagcagttgc agcatcgcat cgacgccgga acgccggccc
     1681 agctgatcgg acagcagcgt ggcatcggcc atcaccatcg cccgggggac ggaaatcagc
     1741 accgcccacg aggtgtcgac ccggccgatg cggatcggcg aatagatctg cagatccggg
     1801 ctcttcgggt cgtcgagcac cactgatttg ccctcggcga tgatgttggc gctgttcgcc
     1861 caggccgcat tggccctggc cgccgcgccg ccgatggtgg ccggatccgc gctgttggcg
     1921 acgatcaggc cgctgtcgtt gatcaccgcc acggtgcttt tgccgttata gagcgacttg
     1981 ctgacctggc tggccagcgt ttgcaggaaa tcgaggttgt agtctgcccc ggccacgccg
     2041 tggaacacgc cgttgatcat gatcggcacc gacatggtgg ccagatagac ctgcttgccc
     2101 tgcacgatat agggcagcgg gcccagcagg ttctcgtggt gggtgttgtg ggggttgata
     2161 taccacgcgc ctttcaccag cccgttggga tggcgcgcgc tgctgtcata ttccaccagc
     2221 ggctggatgg cgattttgcc gttggtgccc cgcgtccaat agggaagaaa gcgcccggtg
     2281 ccgtccgatc ccatcgccgc cagattgcgg aaggcgttgt cgttgccgtc caggccattg
     2341 ggctcccagg cggaataggt gccgttgaag cgggggttct gctcaaggat attgcgcagg
     2401 acagcggtga actgcgtgcg ccggctgccg gcaggcgtgc cgccggcacc gggggcggcc
     2461 atctgctcga agctgttggc ggtgttgcgg gcggcgtcga aggcggtgtc gagttcggat
     2521 ttgatgaaac gggcttccgc attggcgcgg ttctgcaggt tctcattgac ctgctggtta
     2581 atcatttccg aaacgcggcc attcacatat ccgtgggtgc gaacggtcgc aaaggtgctg
     2641 tatgcaacca tgatggcaaa ggcgacgaca atacaagctc ctgctgtcac ggcgatcttg
     2701 gctttgacag agcgcagcat tgtaaatgat ttaagcatga aagcgtccct atcggcagca
     2761 tggccattgc ggtttgtggc catgcgtaca gaggggcgtg gaaagatttg ttgaggtttg
     2821 gggccagtag aaaacctatt ggcctttggt gggagagggg gcgctgccgg gcatggggct
     2881 ggcggggaag accggcgcat ggcagaggca ggaatcggca acgccgggat taaggccgtt
     2941 acgctgcaca gtagggatga tgaggttgca atttacgtgg cggcggccga gcaaaggcgg
     3001 ctcggcaagc agtctatcgc ccgcattgca aaggcagacg tgtctaaccg caaaagggaa
     3061 aggcgtctaa ctcgaaagga aaacactgca aaatgagtgt cttagtatac gaaatggctc
     3121 cctgagtagc agcgatcgaa ccatagctaa gcgccttata tataatcata atatctggat
     3181 ttgcggttga aaggccccct tggaggcccc caaatcaaat cggtggattg ctgagaattt
     3241 cctgcttcca ggcttcgatt tcgctctgca gccaggccac gcggccgccg ccgatggggc
     3301 gcggcgcggg aaaattgccc gctttgattc gccgatagat cgtggctgtg tggaggctcg
     3361 tttccgcgcg gatttccttg atggacatga ggcgtccggg ggcctgcgga actggcggcg
     3421 ggcagggcat gggtttcggc atcggcaggt ctcggcactt gaaatgcggg gagggctggt
     3481 gggggggggc cgatcggcac cggcgcgcga tcaggggccg ctcaccagtc gaactcccac
     3541 agcatgggga tgagggcgcc gggcaggtag agcgggtggg ccggcgtgcc atcgcggttg
     3601 cggcgcaggc tgtgcagttg caggccggcc ttggcgcagg ccaggcgcac ggtgagatcg
     3661 cgctggaaga agtgcccctg ctggccccag ccggcgatcg tgccgccggc cagcgccatt
     3721 gccagggcga tcgccgcatc attgtggggg ccgatggggt tttccgcttc cagcaactcg
     3781 gcgggatcgc gggcgcgcag ggcaaacagg ttccacacca gcatcccatc gagcggcccg
     3841 cagccgggaa tgccccgccg catcacgctg aggcagcggc ggatggtggc atcgttgctg
     3901 gtggcatcgg cggtggaggg gttgagcagg gcaaagccca tgaacggggc ggccatgccc
     3961 gcctcgcgcc acagcagata gcgatagcgc ccgcaggggc tgagcacggc gccggccttg
     4021 gtgccgccga ggaaggagag gccgcgctgc gtagcccaat cgcggatgat gggcagcgcc
     4081 tgtgcggctg gcggcggggc gggggtgctc atgcggcggc cctgcgcgga aagatggcgg
     4141 gggcatctgc gaggatccgc gcgccatagg cgcccatggc cgcgaagaag ggggccacct
     4201 gcaggggatc gagatcgggc ggggcttcgg ccaggatgcc gcccatggtg ttcatggcgg
     4261 caaacagtgt gtcgagctcg tatttcgcca gttccgagag ggcgtaatgc gtctcttgcg
     4321 tcggatccgg ctggcgcgcg aaggcggcgc caaaagctgg gtcgctcatg ggcagggttc
     4381 ctttcgatga acccgacccc caactgcaaa tttggtggcc ggatatgccg gtttgcagac
     4441 cggtagaacc ctgcggtgcc ggcaggccga agcctccgac atatccgacc ataggaaagc
     4501 gcgccgggaa acatccaacg gcgcgccttg tcggcaggga tctggtgcgg ctgcaaatcc
     4561 gcgcccccac cttttgtgtg agggcagcat tggaactgct cctacgcagc caagttgtca
     4621 aggatagcct gtgcatcaag ctatcctcca acggtcgcca cgtaaaattc ggcttatgtg
     4681 tttggtggtc acgccatagc gttgcgaaag caattggcga tctgctccca atttacatag
     4741 ctgacgaatt tcagcagcga tttcaggggt gattttggcg gctgtctcgt tgccatgggc
     4801 acgcctgtcc atcgcatttt gcgatgcggt gccccaagct aaatttacca tgcgattgtc
     4861 agaaggatca ccatttagat gccgaatctg caaatgctca ccggtgggat tcgggagaaa
     4921 tgctagaccg acaaggcgat gaactgactt tgtaaacgac cggccatttc ggcgtaaaac
     4981 gacggaccga tatgactggc cgccagctag gtcagtgttg tgagcacggc cctgccgcag
     5041 cacgcggtga gacagatgac tatatactcg accttcggtg ctgacggcat actctggaaa
     5101 cccttcaatg ggcagccagc tttcaacggt cgcagcggtc acctgttgcg gcgggcaaag
     5161 cgcgggacac acttttgcaa gccgaatggc cgtgtcggcc gcagtcaaaa caagcgcatg
     5221 cgtcatgcga aacatccctg ttgaaggtgc tggcgggagc gggcctccca agcggccaga
     5281 tcctgatcgt attgggtgag gatcgccgcg gctggcgtgg cgcagcggcc gcggctggca
     5341 gacacggcgc ggttcacatc tgcatccagc gctttggccc ggcgcgaaag ttccagcgct
     5401 tcggccagca ggccggcgca gcccgcgcag gccgggcggc ggccggggct ggcggcagca
     5461 gggcgcgctg ccggcgggaa ggccgcgcgg cggctgcgca cgtgatgctc ggcgttcagc
     5521 gcggcgctgt ggcgctggca gggcatgccg gccggcgcgc cgcatttggg gcaggccacc
     5581 gccagcggat cggcattggc gcagggcgcg cacatgaccg atccttcccg gcgctggcac
     5641 ttgccgcagc ggatctccag cgttttcacg cggcgatttc cacgcctgcc tcgaggcggg
     5701 cgagttcggc gcgcaggcgc gcttcggcca cggcgaagtg gcgcgggttg tgctctatgc
     5761 ccacaaagcg cttgccggcg cgcaaggcgg cgatgccggt ggtgcccacc cccatgaacg
     5821 gatcgcacac ggattgcccc gccacgttgc gcatgatgcg ttccatcagc ggcacgggct
     5881 tggggcaggg gtggccgtgc cgatcggcgc cgcgcgggga ggagagccgg cgcacgcgca
     5941 gccgatcggc aatgctgccg gtggggtgcc agccgcgctg ccaggcgtgc acatagaatt
     6001 ccacatcggc ccggtagtgg cggttggcga tgggctgcgg gttggtcttt tcccagatgc
     6061 acagcgcctg ccggtggaag ccgccctgca gccgggtgag cagctcggcc agctgatcgt
     6121 tatgggcaaa gaccacggcc gcgccgcaga gcagcgggtt gatgatgccc atatcgaacc
     6181 cgcggttcag cccatccgtg atgagctggt ccatgttcgg gcggcgcttg cgatagcggc
     6241 cggcaccggt ggcgcggatc aggtagggcg gatcgagcac atcggcatcc atccagccca
     6301 gccggggccg gatggcatag gcatcgcccg gccacagggt gacagggcca aaagtgatcg
     6361 gcttaggcat aggtacgttc atttccgagg cacgggggtg aggttgctgc cgcttcgtgg
     6421 caatcgcggc agcgccaggg gccggcggcg ccgttgcggc tccaggaggg gtggaggagg
     6481 ccgcaatcgg cgcagccttc cggtttccag gcgggcgcag ggccgtggcg gagcgatgcg
     6541 ggcgtgaagg gcgcgcgcct catgccggct ggcccgcaga ggcgaccacc gccgcgctgg
     6601 gcggcgtgtc gggcaggtgg ttgat
//
LOCUS       DASRYE010000119        45394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_130243,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000119 DASRYE010000000
VERSION     DASRYE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 45394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45394
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130243"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="VFF98_06565"
     CDS             <1..1487
                     /locus_tag="VFF98_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZU63325.1"
                     /translation="SAESGHTLTYAGLDRLANQGAHLLRALGLARGDVLAVMLDNDCA
                     AFAIAWAAERAGLYLTSISTRLSLADAAYILADCEAQALVVSDRLAGFGADVARTRPG
                     LPAFTLGAPAAGLASWMAAIAPLPTTPIADESSGTDMLYSSGTTGRPKGVKPALPTGD
                     PGAPTPLVAMGRQLYGMGEETVYLSTSPLYHAAPLRWAMTVMRLGGTVIVMEKFDAEA
                     ALRLIARYRVTHGTWVPTHFVRLLALPEAVRRRHDLSSLARAIHAGAPCPVPVKQAMI
                     DWWGPIIDEYYSGTEMCGITALDAREWLERPGSVGKAVLGRLHILDDDGRELPCGTPG
                     NVYFSDGPAFAYHRDPAKTAAAHNEKGWATLGDIGKVDEAGYLYLTDRKNFMIISGGV
                     NIYPQEIENCLLTHPLVADAAVVGCPDPEMGECAVAVIQLAPEAVAGPHTTADIRDFL
                     RARLGGVKTPRRFEFRAELPREPTGKLLKAALVAEFRGRTSQQP"
     gene            1502..2191
                     /locus_tag="VFF98_06570"
     CDS             1502..2191
                     /locus_tag="VFF98_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU63326.1"
                     /translation="MHIAEAIATRRSVRAYLDRPVPLETIRRVLDLARWAPSGCNYQP
                     WEATVLTGAPLQALQEKLLAAQPDDPLEYDWAAPGKHARYQARLQAVGAAMYGALGVA
                     RDAPGQREAFARANMTSFGAPVLLLCHFPKFMKEPQWSDVGMWLQSIMLLLRGEGLDS
                     CPQEYMGNYGRTIKAQLGLSDETMLFCGLAIGWRDEQAPVNAFARERVALDDQVAFLG
                     FDAALNAALPA"
     gene            2361..3368
                     /locus_tag="VFF98_06575"
     CDS             2361..3368
                     /locus_tag="VFF98_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZU63327.1"
                     /translation="MPLPSATRRRLPGLEALRCVAALCVLALHLRAVFGGRYVFGRGY
                     LGVDFFLMLSGYLMARTQEARLAAGASPWGFLLRRYGRLWPLMALGSLPGVWVQALAL
                     GVVSAAVWWRYALTATLNLLLLPDMQTYFAFPINIPAWTIFFELVMNALHVLLFWRLR
                     GGWLALVLAALLPVVVGMALRNNGLDVGARPETFLLGGLRVGFAYLIGVALYRWWRDV
                     PVLPVPPLLAIALMPALLPLLFALRIWSWWPDVAFVLLVGPLMIAGALRLAPQGAPAR
                     WSTLAGQLAFPLFAFQMPILQGFHDQSWGYWPAAAAVLAVGVSGACILAWLAARKGTA
                     A"
     gene            3365..4168
                     /locus_tag="VFF98_06580"
     CDS             3365..4168
                     /locus_tag="VFF98_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZU63328.1"
                     /translation="MTEPVLITREEGGITLLTLNRPALRNPISDPDMLEALLAALADA
                     DADPATRVIILTGAGGAFSSGGNLNAMRPGAGLIDAAPVGTRQNYRHGIHRLPLLMAR
                     LEVPVIAAVAGPAAGAGCDLACMADLRIAGQSALFIESFVKIGLISGDGGAWLLPRVV
                     GFAKAAEMALTGDPVDAPTALACGLVSQVVPDADLPQAALALARRIAANPPHAVRMTK
                     RLLWEARRADLATILEMAGAMQSLAHATNDHAEAVDAFLAKRPARFTGS"
     gene            4225..5511
                     /gene="ribB"
                     /locus_tag="VFF98_06585"
     CDS             4225..5511
                     /gene="ribB"
                     /locus_tag="VFF98_06585"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZU63329.1"
                     /translation="MSNRIIEQVRQLVADGQLTKAGIARAAGLHANTLRDCTEPDWNP
                     TAETLAKLERFLAETDERPVLVGIEEIIEEARNGRMYILVDDEDRENEGDLIIPAQMA
                     TPQAINFMATHGRGLVCLALSAERVEQLGLEPMSRRNGTPLGTAFTVSIEAKEGITTG
                     ISAGDRARTVSVAIDGSKGQADIVSPGHVFPLRARPGGVLVRAGHTEAAVDISRLAGL
                     NPSGVICEVMREDGTMARLDDLIGFARRHGLKIGTIRDLIAYRRQHDRMVELSREITF
                     TSRHGGEWLARSYRNRATGEDNLALVKGRIDPDQPTLVRMHVLSVFSDILGEVSERAE
                     LLHRSMEIIAEAGAGVIVLINKPLPRSFSRAIEIKEQLRTGNTQAMEELRDYGVGAQI
                     LTELGVHDMILLTNTSHTLVALEGYGLSISGEQRID"
     gene            5526..5948
                     /gene="ribH"
                     /locus_tag="VFF98_06590"
     CDS             5526..5948
                     /gene="ribH"
                     /locus_tag="VFF98_06590"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZU63330.1"
                     /translation="MAKFLIVEARFYDHLNDMLVAGAKAALKAAGHKADVITVPGALE
                     IPGAIALADQAGEYDGYVAIGVVIRGETYHFEIVAGESARGVMALSMDGLAIGNGILT
                     VENEAQAIVRADPAQKDKGGEAAKAALALLALRERFGA"
     gene            6200..6454
                     /locus_tag="VFF98_06595"
     CDS             6200..6454
                     /locus_tag="VFF98_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63331.1"
                     /translation="MNDLSLPSAAPMPRLASAVFPERNMVFNTLTDCLNRLDMLGEQL
                     AAAHLAACLDSLRETFIMDGNISDMDIHRRRRNLDIAKSD"
     gene            6564..7064
                     /locus_tag="VFF98_06600"
     CDS             6564..7064
                     /locus_tag="VFF98_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HZU63332.1"
                     /translation="MTQEIASSYTSTEVATQLLGITQSLDVLASQLHSIAGQNRPVLK
                     QASARRELPPSAEQLAKIAGQAYRDRRRRTELFGDETLFGEPAWDILLDLFVAAIKHK
                     RVAVTSACIGSAVPTTTALRWLKILEEKGLILREEDPDDARRTFLRLSPGAYDLMVDY
                     FHPEQK"
     gene            complement(7133..8419)
                     /gene="eno"
                     /locus_tag="VFF98_06605"
     CDS             complement(7133..8419)
                     /gene="eno"
                     /locus_tag="VFF98_06605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZU63333.1"
                     /translation="MTAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTG
                     AHEAVELRDGDKARYLGKGVLKAVEAVNTEIHEALVGLDAEDQRDIDLAMIELDGTEN
                     KGRIGANAILGTSLAIAKAAADARGLPLYAYVGGVAAHVLPVPMMNIINGGEHADNPI
                     DFQEFMIMPVGADSIAEGVRWGAEIFHTLKKGLHEKGLATAVGDEGGFAPNIASTRAA
                     LDFVSASIEKAGFKLGTDILLALDCASTEFFKNGKYEISGEGLSLSPVEFAEYLARLA
                     ADYPIISIEDGMSEDDFEGWKALTDLIGDKVQLVGDDLFVTNPKRLTMGIEQGLANSL
                     LVKVNQIGSLTETLEAVSIAQRAGYTAVMSHRSGETEDATIADLAVATNCGQIKTGSL
                     ARSDRLAKYNQLIRIEEQLGAASRYAGKAAFGRLLR"
     gene            8619..9167
                     /locus_tag="VFF98_06610"
     CDS             8619..9167
                     /locus_tag="VFF98_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63334.1"
                     /translation="MSEPETITAVTDAVAVASPAEGPAEGDAKARFSKALDEARAGAV
                     ALGKDVQGKAGAYADRLGAKGHDLLDDAKALTEEAKDRAATLADEGKVRASEALVSLG
                     KLVEENAALVEEKLGGKYGEYFRTAAQHINDAAARIEAKDLGELGEDARTFVREKPAL
                     AVGLAAAGGFVLARLFGGKSSK"
     gene            9200..9631
                     /locus_tag="VFF98_06615"
     CDS             9200..9631
                     /locus_tag="VFF98_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63335.1"
                     /translation="MRNLFSARPPHDAEEGEPHPGSQADAGADAGEDSLGALLRGLIA
                     ALGQLWQAEIAYQRARARALAGMIGMIAALLVAAVILALALLVAVSVGLVWALTARLG
                     AWGAMGAVALGLLLLLALVLVLAGRMGRRIARVIFGGGLRG"
     gene            9628..10053
                     /locus_tag="VFF98_06620"
     CDS             9628..10053
                     /locus_tag="VFF98_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63336.1"
                     /translation="MSRLEKDLAAARAARDAAQAELTARLGQARAAVHPARLAGRLKR
                     EAGARAQDIAGQALEIATDHRGILVATVSATLMWLGRKQIGKLATRHLPVERYAALAK
                     SLGPQALQGARGKRLARQVSLWGPRAWRLWKRLRLPGAR"
     gene            10189..10404
                     /locus_tag="VFF98_06625"
     CDS             10189..10404
                     /locus_tag="VFF98_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HZU63337.1"
                     /translation="MQKLHLVMGGRVADPQGLEFVDLKALDLVGVFPDYAAAVEAWRG
                     AAQRSVDDAEMKYVIVHLHKLLEPDAE"
     gene            complement(10501..10977)
                     /gene="greA"
                     /locus_tag="VFF98_06630"
     CDS             complement(10501..10977)
                     /gene="greA"
                     /locus_tag="VFF98_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZU63338.1"
                     /translation="MASVEKLPMLAEGYEKLIAELKVLREERPKIVEAIEEARAHGDL
                     SENAEYHAAKERQGQVEASIGDLEDKVGRAQIIDPTTLSGDRVVFGATVTLLDDDDKP
                     VRYQIVGPYEADAKVGRISYNSPLGRALIGKRIEDEIEVSVPAGERFYVVEKIEFI"
     gene            complement(11093..14407)
                     /gene="carB"
                     /locus_tag="VFF98_06635"
     CDS             complement(11093..14407)
                     /gene="carB"
                     /locus_tag="VFF98_06635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZU63339.1"
                     /translation="MPKRTDISSILVIGAGPIIIGQACEFDYSGTQAIKALKEEGYRV
                     ILVNSNPATIMTDPEMADATYIEPITPEIVAKIIEKERPDALLPTMGGQTALNTALAL
                     FNDGTLAKFGVQMIGADADAIDKAEDRQRFREAMDKIGLESARSGVAHTVEEAFAVLE
                     TTGLPSIIRPSFTLGGTGGGVAYNKAEFEKIVRSGLEASPTTEVLIEESLLGWKEYEM
                     EVVRDRHDNSIIICSIENVDPMGVHTGDSITVAPALTLTDKEYQIMRNASLAVLREIG
                     VETGGSNVQFAVNPADGRLIVIEMNPRVSRSSALASKATGFPIARVAAKLAIGYTLDE
                     ITNEITGATPASFEPTIDYVVTKIPRFAFEKFKGAEPILSTAMKSVGEVMAIGRNIKE
                     SMQKALRGLETGLDGFNRVDHLVGASRDEITAALSRATPDRLLVVAQALRAGLSVDDV
                     HAITHYDPWFLRQIAEIIAEEAKIIADGLPNDAAGLRRLKAMGFSDKRLAKLAVESVH
                     MVGSANVARAGLVHDVVEAMAGATSEAEVRALRHKLGVHPVFKRIDSCAAEFEAVTPY
                     MYSTYEAPLFGEAENEAEPSERRKIVILGGGPNRIGQGIEFDYCCCHACFALADAGYE
                     TIMVNCNPETVSTDYDTSDRLYFEPLTAEDVLEILRVEQSKGDLVGVIVQFGGQTPLK
                     LAQALEDAGIPILGTSPDAIDLAEDRERFADLVEKLGLSQPANGIARSRDEAVAVANR
                     IGFPVLMRPSYVLGGRAMEIVDSVAQLDDYIATAVQVSGDSPVLIDQYLRDAIECDVD
                     ALCDGEDVVVAGVMQHIEEAGIHSGDSACTLPPYSLGADIIAEMERQARALALALGVK
                     GLMNIQFAVKEGEVYLIEVNPRASRTVPFVAKAIGQPVAKIASRVMAGEKLASFPPFK
                     RDLPYMAVKEAVFPFARFPGVDPVLSPEMKSTGEVMGIDKGFATAFAKAQLGAGTKLP
                     QGGVAFLSVKDTDKAVILPAARQLVELGFQLVATGGTAKFLAAEGVPVQQVNKVAEGR
                     PHIVDRIIDGDVALVVNTTEGWQSLKDSQSIRQAALAGKVPYFTTASASVAAAQAIAA
                     LRATSLEVSPLQAYYN"
     gene            complement(14407..15573)
                     /gene="carA"
                     /locus_tag="VFF98_06640"
     CDS             complement(14407..15573)
                     /gene="carA"
                     /locus_tag="VFF98_06640"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZU63340.1"
                     /translation="MVDPAPLLAPKPAGATAVLVLADGHVAWGRGFGAVGSAVGEVCF
                     NTAITGYQEIMTDPSYAGQIVTFTFPHIGNVGVNEEDLESHVDGAVGCVVREDVTAPS
                     NFRAESDFSAWLEARGKIGIAGIDTRALTRRIRLSGAPNAVIAHNPVGNFDIPALLAK
                     ARAWPGLEGMDLAKVVTREGAEDWHGGTWALGEGYAQPAEDGRPHVVAMDFGAKDNIF
                     RNLVAAGARVTVVPAETGLEAILALKPAGVFLSNGPGDPAATGVYAVPVIRALLDAGV
                     PIFGICLGHQLLALAAGGKTIKMHQGHRGANHPVKRLAEGVVEITSMNHGFAVDAASL
                     PAGVVETHVSLFDGSNCGLEIAGKQAFSVQYHPEASPGPQDSLYLFTKFIESLA"
     gene            15784..16236
                     /locus_tag="VFF98_06645"
     CDS             15784..16236
                     /locus_tag="VFF98_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZU63341.1"
                     /translation="MIRDTIKAAQVAAMKSGDKARLAAVRLILAKLKDKDIELRTAAS
                     LPDDDTLVTDVLQKMMKQRRESIAMYEQGGRPELAAAEAAEVAVIEDFLPAQMGEDEV
                     KAAIEAIKAELGAAGPKDMGRVIAELKARHGTVIDMGKASALVKAALA"
     gene            16242..18194
                     /gene="dnaG"
                     /locus_tag="VFF98_06650"
     CDS             16242..18194
                     /gene="dnaG"
                     /locus_tag="VFF98_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZU63342.1"
                     /translation="MALTPQWLDELRSRITLSGVIGRTTRIQKAGREFKACCPFHNEK
                     SPSFTINDEKGFYHCFGCGAHGDVIRWMTDQRGLSFMDAVKQLAEEAGMEVPAADPHT
                     ARLSAQRAEQAKTLHDVMAAAQDWFAANLAGPEGAEARAYLATRGFSAEVCRQFGFGY
                     APEGRQALKGALDRFPEAMLVEAGLRIAVEEREPYDRFRGRLMLPIQDARGRVIAFGG
                     RILSAQKTDAPKYLNSPDTPLFDKGRTLYNLHRAAPIARQSGRLLVVEGYMDAIALSA
                     ASFGDCVAPLGTALTETQIELLWRQVPAPILCFDGDKAGLRAAAKAMVRALPLLRPGH
                     SLRVLSLPAGQDPDELLKARGPQAMDALLEGAQGLADALWQHERDALPLAGPEDKAGL
                     KARLMAHVDAIAHPDIKALYRREWLDRYGEFAFARPERPAPAPRPQARSGGGRRGSWR
                     DAPPPAPLPETAARLARPAASVAGRARAITMVVLGLMRNPAALARLAEPLARLAPEEP
                     RIDALLDALDYGLPLEGADIATIFAGRALAEPSLPGPREAVFAFVAESGEAAMAELAE
                     VVAVLVERPAIEEALAQAQARFEAELTEEAGEEVTRLRTLLLALQKRELDLNARLGQM
                     ATGNADRATGTGPEAAGADAGNKMAE"
     gene            18194..20230
                     /gene="rpoD"
                     /locus_tag="VFF98_06655"
     CDS             18194..20230
                     /gene="rpoD"
                     /locus_tag="VFF98_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZU63343.1"
                     /translation="MDEEISGTDMGGVDKGEGGDAPLIDLNEASIKKLIARAKRRGII
                     TYDELNEALPQDQMSSEQIEDIMAAISEMGVNIVESDEDNAEPDDAGNDDVEDSDAMV
                     SDRPAPEKKRETIERTDDPVRMYLREMGAVELLSREGEIAIAKRIEAGRDTMILGLCE
                     SPITFHAIIQWSEALNNGEMQLREILDLDAMLSKDPAPENLSEDGEDDGDGEISEATA
                     GPSYKEEEDIEEEESADGEDEDGIERRARPPAEDDEDDNTLSLAQMEAALKPEALEKF
                     AQITELFHAFERIQAERLEAMAIGNDFAADKERQYQQLREDLTAQVESVQFHASKIEY
                     LVDNLYAFNRRLTTLGGQMLRLAERHKVSRKDFLDAYVGRELDDAWLTSVAGKDKKWG
                     NFAANEGASVERIRAEVSDIASQTGMALPEFRRIVNMVQKGEREARIAKKEMVEANLR
                     LVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSI
                     ADQARTIRIPVHMIETINKLVRTSRQFLHEQGREPTPEEMAERLSMPLEKVRKVMKIA
                     KEPISLETPIGDEEDSHLGDFIEDKNAIIPVDAAIQSNLKETVTRVLASLTPREERVL
                     RMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKMRSFLDQ"
     gene            20275..20991
                     /locus_tag="VFF98_06660"
     CDS             20275..20991
                     /locus_tag="VFF98_06660"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HZU63344.1"
                     /translation="MRTSASRLAALIAVAAATIATPVLAKAAKPAPAPQASPAAAAPA
                     QPAAPALPPVPAASLDLARKIVAIFFPADQQEAVVRNSVAGLMPRYRAAVALPKGWDD
                     PALKAMVEASYPQMQQKLQPIVAAGLPHLYDALAEAYAREFTTAELTDIVAFSATPAG
                     RHYVLASGRINGYPMVVAANNATLTEAAKAEQQQTQLVVAEVKDYLAKHPDVMARIKA
                     QAAAKPAAAPHPQAAGTTER"
     gene            21126..21563
                     /locus_tag="VFF98_06665"
     CDS             21126..21563
                     /locus_tag="VFF98_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZU63345.1"
                     /translation="MTIALASDHAAVALKAELVAYLAQLGHAPIDLGPADDSRVDYPD
                     YGYKLAEAVASGVARFGVALCGSGIGISIAVNRHPALRCALVSEPLSAALAREHNNAN
                     AIAMGARLTGPDMAKACLDAFLSAEFGGGRHEGRVEKLSNPAF"
     gene            21587..22894
                     /gene="glyA"
                     /locus_tag="VFF98_06670"
     CDS             21587..22894
                     /gene="glyA"
                     /locus_tag="VFF98_06670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZU63346.1"
                     /translation="MTATTDVRPQGFFTGTLAERDPAIAGWINKELGRQRDKIELIAS
                     ENICSTAVLEAAGSILTNKYAEGYPGKRYYGGCEYVDEIETIAIERAKQLFGSQFANV
                     QPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFHPVPYGVRADD
                     HRIDMDEVARIARECSPKLIICGGTAYSREWDWARFREIADEVGAYLLADMSHISGLV
                     AGGVHPSPVPHAHVTTTTTHKSLRGPRSGIILSNDEAIAKKLNSAIFPGLQGGPLMHI
                     IAAKAVALAEALTPEFKAYAAQVKANAAALAASLQAEGLGIVSGGTDNHLMLVDLTPY
                     GAKGRHAEHALDRASITCNKNSIPFDPEKPMLTSGIRLGTPAGTTRGFGPAEFTQIGK
                     WIAQVVDGLRKNGAEGDAQIEARVAAEVEALCAGFPIYPALRY"
     gene            22898..23389
                     /gene="nrdR"
                     /locus_tag="VFF98_06675"
     CDS             22898..23389
                     /gene="nrdR"
                     /locus_tag="VFF98_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZU63347.1"
                     /translation="MRCPFCAFDNTQVKDSRPTEDNTAIRRRRQCESCGARFTTFERV
                     QLREITVIKACDPESGEARREPFVREKIEQSVALACRKRGLAQERLDQLVSGIQRQIE
                     TLGESEVPSRVIGEMVMDGLRGLDSVAYIRFASVYRDFAAPEDFEEFASAIRDAGAGK
                     EPA"
     gene            23386..24177
                     /locus_tag="VFF98_06680"
     CDS             23386..24177
                     /locus_tag="VFF98_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZU63348.1"
                     /translation="MSQPPVIVLVRPQLGENIGKAARAMLNFGLADMRLVAPRDGWPN
                     PSAGPAAAGADIVLEKAQVFATLAEAVGDCAHVYATTVRKRGVTKPVLTPEEAAREIH
                     AHDGADLGRSALVFGPERAGLETEDVALARAIVTVPVNPEFASLNLAQAVILAAYEWS
                     KGVNRAAGTLAQPTQEELLPPAPQAELEGMIAHAETLLAERDYFFPPHRAEATRLTLR
                     GLFTKPAWNHLEVRTMRGVLAALVRRPGEVPPPRPRPAPKADDQA"
     gene            24344..24958
                     /gene="rpsD"
                     /locus_tag="VFF98_06685"
     CDS             24344..24958
                     /gene="rpsD"
                     /locus_tag="VFF98_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZU63349.1"
                     /translation="MSKRHASKYKIDRRMGENIWGRPKSSVNRRSYGPGQHGQRRKGK
                     ISDFGIQLRAKQKLKGYYGDVTEKQFKRTYQEASKMKGDTGQNLIGLLEQRLDMVVYR
                     AKFAPTIFSARQIVSHGHIRVNGVKCNIASRRVRPGDIISLGNKAKEMALIAEAQALP
                     EREVPDYVAQDSTDKVTFVRVPTLDEVPYPVKMEPNLVVEFYSR"
     gene            25107..25346
                     /locus_tag="VFF98_06690"
     CDS             25107..25346
                     /locus_tag="VFF98_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63350.1"
                     /translation="MLYRYVGAQRAGKWYPDLATAQRFACKIGAGFLAEKSGQFCAYL
                     GTRLEVLLPDGRVIRRRMRAPQPAGHFPPGHFPAK"
     gene            complement(25328..26455)
                     /gene="tgt"
                     /locus_tag="VFF98_06695"
     CDS             complement(25328..26455)
                     /gene="tgt"
                     /locus_tag="VFF98_06695"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZU63351.1"
                     /translation="MTRFSFQIHARDGKARTGAIQMLRGDIRTPAFMPVGTAATVKAM
                     KPETVRGTGADIILGNTYHLMLRPGAERVARLGGLHKFMNWPRPILTDSGGYQVMSLS
                     DLRKITEEGVTFASHIDGSRHMLSPERSMEIQRLLGSDIVMCFDECPRADQSRDVIAA
                     SMEMSMRWAARSRAAFNAGGEHAERAALFGIQQGALHEDLRRISAEKLTEIGFDGYAI
                     GGLAVGEGQEAMFATLDFAPGMLPDDKPRYLMGVGKPDDLVGGVERGVDMFDCVLPSR
                     SGRNGQAFTWNGPLNIRNARHAEDTGPIDERCSCHVCQTYSRAYLHHLNKAGEILGAM
                     LMTEHNISFYQQLMAAMRGAIAEQRFAAFAADFRRDYFAGK"
     gene            26497..27252
                     /locus_tag="VFF98_06700"
     CDS             26497..27252
                     /locus_tag="VFF98_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZU63352.1"
                     /translation="MLDPWIYPALTATAVFTGFVDSVAGGGGLIMMPALIYAGVPPTM
                     LLGTNKVQSACGTAMATWRYHRAGLFALKPNLPAVAAIFAGAAIGAAVIQSFNANVLK
                     LVVPVLLMMVSLYTLFSPRMHDEDSHGHMSQKGFLPVGGALGFYDGFFGPGAGQFYAT
                     ALVSLRGLGLTRATGLTKLFNVTSNIASILVFAAGGHVIWLLGLCMGAGAMTGNWIGA
                     HTATRFGAKLIRPLLVVCSLGMTARLLWNWFAG"
     gene            complement(27256..28302)
                     /gene="queA"
                     /locus_tag="VFF98_06705"
     CDS             complement(27256..28302)
                     /gene="queA"
                     /locus_tag="VFF98_06705"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZU63353.1"
                     /translation="MQVDLFDFDLPAERIALRPARPRDAARLLLVDGAGGGGLDDLRV
                     RDLPALLRAGDVLVFNDTRVIPAQLEGRRGEAKIGATLHKRIDLRRWQAFVKNGKRLH
                     EGDVIAFPAGVEALAEARHADGSWTLAFAGEEPVELLLERAGTMPLPPYIAGKRPTDE
                     ADRADYQTMFAARDGAVAAPTAALHFTPELIAALDGAGIARECLTLHVGAGTFLPVKA
                     ENTEDHAMHAEWGTLPADVAERLNAARAAGGRVIAVGTTSLRLLESATGEDGVIRPFA
                     GDTSIFITPGYRFRAIDGLMTNFHLPRSTLFMLVSALMGLERMQGAYAHAIAEGYRFY
                     SYGDSSLLLPGGRG"
     gene            complement(28321..29028)
                     /locus_tag="VFF98_06710"
     CDS             complement(28321..29028)
                     /locus_tag="VFF98_06710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU63354.1"
                     /translation="MNRHPLARIALGLSMGLVLLSTSACGHKTATLHLAKAGPAPTGK
                     LLSAQVDTDIAHDPDNVLLLDLSNGGRVAIRLMPEWAPHHVERIKELARQGFYNGIIF
                     HRVIDGFMAQTGDPTGTGGGGSKLPNLKAEFNTVPHVRGSVSMARTDDADSANSQFFI
                     VFYPHMALDRHYTNFGRVIAGMDVVDGIERGEPPANPTRIVQASVASQHLAPPPPTAI
                     KAVAPISIDQLNHSKSN"
     gene            complement(29197..29709)
                     /gene="coaD"
                     /locus_tag="VFF98_06715"
     CDS             complement(29197..29709)
                     /gene="coaD"
                     /locus_tag="VFF98_06715"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZU63355.1"
                     /translation="MAERVGVYPGTFDPITLGHADIIRRGAKLVDRLIVGVTTNPSKN
                     PLFPPVERIAMVEREVAAMGLENVSVVGFNALLMKFAEKQGASVIVRGLRAVADFEYE
                     YQMAGMNQQLNARIETVFLMADVSLQPIASKLVKEIALFGGEIGKFVSPAVRTDVIER
                     IVATGGVQGD"
     gene            complement(29710..30612)
                     /locus_tag="VFF98_06720"
     CDS             complement(29710..30612)
                     /locus_tag="VFF98_06720"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZU63356.1"
                     /translation="MAAQPPSLLAESLKRVQREIDAAFDALLPETDDPRARLIAAMRY
                     AAIGGGKRVRPLLVAATAELFGVDREAAIRVGVALESVHVYSLIHDDLPCMDNDDLRH
                     GKPTVHKAFDDATAVLAGDSLLTFAFEVLTDPATSGDPFTRVELVQTLALASGHHGMA
                     GGQMMDIVAETSTFDLATITRLQQLKTGALLSAGVEMGAILGHVPPEGRTHLRGYARD
                     IGLAFQIADDLLDYEGNEEAAGKALRKDEAKGKETFVSLLGPERAREQARLLLDQSIA
                     HLAQYGPEADLLRELARFIVERDR"
     gene            complement(30650..30916)
                     /locus_tag="VFF98_06725"
     CDS             complement(30650..30916)
                     /locus_tag="VFF98_06725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZU63357.1"
                     /translation="MTQDAADMAARIAALSFEEALRALEDVVRRLESGEVPLDESISL
                     YERGEALRLACQARLDAAAARIEKIVAAPPGGAPQATRPFDQDA"
     gene            31028..33181
                     /gene="purL"
                     /locus_tag="VFF98_06730"
     CDS             31028..33181
                     /gene="purL"
                     /locus_tag="VFF98_06730"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZU63358.1"
                     /translation="MSTQDSAITPDVVAAHGLSEEEYAKVLHALGRTPNLVELGIFSV
                     MWSEHCSYKSSRLHLKKLPTEAPWVICGPGENAGVIDIGDGQAAIFKMESHNHPSYIE
                     PYQGAATGVGGILRDVFTMGARPVANMNALRFGRPDHPKMKHLVQGVIAGIGGYGNCV
                     GVPTVGGETNFHKAYDGNILVNAMTVGVADTDKIFYSAATGLGNPIVYVGSKTGRDGI
                     HGATMASADFDEKSEEKRPTVQVGDPFTEKLLIEACLELMATDAIVAIQDMGAAGLTS
                     SSVEMASKGGAGIRLNMNNVPCRETGMTPYEMMLSESQERMLMVLQPGKEPMAEAIFK
                     KWELDFAVIGEVTDTGHMVLEFDGEVVCDIPLAPLADEAPLYDRPHVPAPKPAPLADA
                     PASADIAGDLLKLIGSPDLASRRWIFEQYDSQVGGDTLQKSGGDAAVVRVHGTDKALA
                     ISTDCTPRYCFADPYEGGKQAVAETFRNICAVGATPLAITNCLNFANPQRPEIMGQIV
                     GCLEGMGDACRALDYPIVSGNVSLYNESKATGGGSAILPTPAIGGVGLMLDHTKMATI
                     RFKAPGETIWLIGPEGTHLGQSLWLREIAGREEGPAPAVDLELERRNGETVRQLIHDG
                     IATAVHDVADGGLLVAIAEMAMASGLGAELDGSFTAATAFGEDQSRYVLTTPADIVLE
                     GAIRLGRVGGASVAGVEVTRLKAANEAFFKDWMEA"
     gene            complement(33228..34109)
                     /gene="serB"
                     /locus_tag="VFF98_06735"
     CDS             complement(33228..34109)
                     /gene="serB"
                     /locus_tag="VFF98_06735"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZU63359.1"
                     /translation="MVIARVIADSDGLSARVEALRATLRSQGRDSAARVLENGPAALL
                     ELESDAGADALRSAIEAHLGQTDALIGAGVPQLPALFVSDMDSTMISAECIDELADFA
                     NLKPQIAAITERAMQGELDFRAALAERVALLKGLSEGAIARCLAERIHPMQGARTLVQ
                     TLKAKGSRTVLVTGGFHQFADPVAEALGFERVVGNRLGVEGGVLTGGLVGPISDSATK
                     KATLLEELAALGEGAHTLATGDGANDIPMMEAATYGIAFAAKPKARAAANGWIDRGDL
                     TAVLRLLGVGESEWVKG"
     gene            34102..35064
                     /gene="miaA"
                     /locus_tag="VFF98_06740"
     CDS             34102..35064
                     /gene="miaA"
                     /locus_tag="VFF98_06740"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZU63360.1"
                     /translation="MTTNTIPETDRGPGRPPLALIAGPTASGKSDCALALARWLAARG
                     RQAVIVNADSAQLYADLPVLSAAPSPAEQAEVPHRLYGAWDGAEGCSAADWAARARDE
                     IDVAHKAGMVPILVGGTGLYIRTLLDGIAPVPAIDPAVRAAVRALPVAQAHAALARED
                     PAAARRLHPADTTRVARALEVVRATGLPLASWQARKEGGIGHRVALAPVVLLPERGWL
                     YARCDRRFERMVEAGAVAEVAALLARGLAADLPVMRAIGVAEIAAHLAGDSDLATAIA
                     RGQQATRNYAKRQYTWLRHQPPQDWPRQTSSDIDFSPYFEILLP"
     gene            35268..37010
                     /gene="ilvB"
                     /locus_tag="VFF98_06745"
     CDS             35268..37010
                     /gene="ilvB"
                     /locus_tag="VFF98_06745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZU63361.1"
                     /translation="MSDERSGAQILVESLVRQGVEHVFGYPGGAVLPIYDALFGDERL
                     RHILVRHEAAAAHAAEGYARSTGRPGVVLVTSGPGATNAVTGIADAFLDSIPMVVITG
                     QVPTALIGSDAFQEADTIGITRHCTKHNYLVKDPADLAATIDEAFQVATSGRPGPVVI
                     DIPKDVQIASAVLSDAPVQRRQRYAPQTRGADKDIAAAIELLAHAKAPVFYTGGGVIN
                     SGPRATELLRQLQAIIGAPVTSTLMGLGAFPATHADWLGMLGMHGTYEANWAMNQADL
                     ILCVGARFDDRVTGRLDAFAPNSTKIHIDIDRASINKTVPVDLPIIGDCAEVLEQLIT
                     AWGNRKPQDLAEWKARVDGWRAKKCLAYPAYGSEIAPQRAIERLHALTHAVDPIISTE
                     VGQHQMWAAQYFGMEEPNRWLTSGGLGTMGYGLPAAIGAQVGNPGRLVIDIAGEASIQ
                     MNIQELGTASQYRLPVKVFILNNEWMGMVRQWQELTYESRYSNSYSDSLPDFVRLAEA
                     YGWKGIAITSEAELDAGIQAMIDHDGPVIVDCRVAKAANCFPMIPSGAAHTEMLLPGD
                     VVAGTMDDEAKALV"
     gene            37026..37541
                     /gene="ilvN"
                     /locus_tag="VFF98_06750"
     CDS             37026..37541
                     /gene="ilvN"
                     /locus_tag="VFF98_06750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZU63362.1"
                     /translation="MKIKHAAEERHVLTVLVDNEAGILAKIAGLFTARGYNIDSLTVA
                     DITDNHDVSRITIVTHGPPAVIDQIRAQLDRLVPVHKVIDLTEVGPHVERELALIKVA
                     GKGENRVEALRIADLFRAKVVDTSTSSFIFELTGTPDKIDSFIALMRELGLVEVGRTG
                     IVGMTRGGQPA"
     gene            37702..38721
                     /gene="ilvC"
                     /locus_tag="VFF98_06755"
     CDS             37702..38721
                     /gene="ilvC"
                     /locus_tag="VFF98_06755"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZU63363.1"
                     /translation="MKVYYDADADLNLITGKKIAILGYGSQGHAHAQNLRDSGVKDVA
                     IALREGSATAKKAEAAGFKVLSNAEAAAWADVLMILAPDEHQAAIYAADLHANMKPGA
                     ALAFAHGLNIHFGLIEARADIDVIMIAPKGPGHTVRGEYQRGGGVPCLIAIHQDVTGN
                     AHDIALAYASGVGGGRSGIIETNFREECETDLFGEQAVLCGGTTALVQAGFETLVEAG
                     YAPEMAYFECLHELKLIVDLMYEGGIANMRYSISNTAEYGDIVTGPRIITEETKKEMK
                     RVLADIQSGRFVKNFVLDNRAGQPELKASRIAAKRHPIEETGAKLRAMMPWIGANALV
                     DKSKN"
     gene            38850..39971
                     /locus_tag="VFF98_06760"
     CDS             38850..39971
                     /locus_tag="VFF98_06760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU63364.1"
                     /translation="MPHRADHILICFHDFSRGGTERIAIGLARHWAEQGRRVTILCGT
                     TEGGLRATVDARVQVVELDPPVRRSLTSRLKLGKAMAAEIARLKPDIIFLPGNFHLPL
                     APAFARIPGRVEEGWGAIMAKISNPSVPDGLAAAPVRWLLRRFGPCVDGWAAMNSGLE
                     RELHAILPGAHVETLFDPVYLHHDLAPDDHLAPTDERLEILWAGRFEPQKDTLLALKV
                     IEALNEIVPARLTMVGDGRLYPATLRAIKRMGLTDIIAAPGYVPGIDPWLRRADALLI
                     TSHFEGGPAVAVEALAHGVPVVSTDCSHFLRDIMRVPLAGRIVPSRYAPDLASALLST
                     VSEGRRDPAALHALIDHLDPRVCAQGYLAWFDRMAVARR"
     gene            40218..41894
                     /gene="leuA"
                     /locus_tag="VFF98_06765"
     CDS             40218..41894
                     /gene="leuA"
                     /locus_tag="VFF98_06765"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZU63365.1"
                     /translation="MSMLNDPSVKYRPFPQVNIPDRQWPSRTITKAPRWLSTDLRDGN
                     QSLIDPMGAEKKTRFFDLLVKVGLKEIEVGFPSAGATEFDFIRGLVDNGRVPDDVLVQ
                     VLTQSREDLIRTSFESLAGAPAAIVHVYNAVSPLWRQVVFGMEKPEIKEIARNGARLL
                     RDNAARFPQTKWHFEYSPETFSTAELDFSIECCEAVMEILQPTAEHPIILNLPATVEA
                     STPNIYADQIEYFCRNLPGRDRAIISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGN
                     GERTGNCCLVTVALNLYTQGVNPELDFSDIDEVIQTVEYCNQLPVHQRHPYGGELVFT
                     AFSGSHQDAIKKGFAAQEQRNDQLWAVPYLPIDPADLGRSYEAVIRVNSQSGKGGFAW
                     VLEQDQGLKLPKRMQAHFSRHVQQLADELGRELQAADIWDVFQRTYRVNDPQHLQLLD
                     WDEARGADGSRIFAGKIGVDGQEQSVSGRGNGLISSVVATLADSFGVALSVKDYSEHA
                     MGAGTDARAAAYVECTGPDGQTVWGVGLDTDVATASVRAVLSAANSLIGR"
     gene            complement(41902..42558)
                     /locus_tag="VFF98_06770"
     CDS             complement(41902..42558)
                     /locus_tag="VFF98_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HZU63366.1"
                     /translation="MGPGAPRHHVLPAALPACGLFLPLSAGQEAAGHEAGQETARRRL
                     PFRAVNRRDAADYDPGLQRHHLLPRQLLQGRCFGTLFEAIGRERLGFDDFRANGLLLP
                     ATDAGAARIGLPLHRGPHRDYNAMVTERVGQVEADWSCRRLRAPEVALSEALARLRLL
                     QRALRRRLLDPGRRPLRLNRAQPVLYNADFAELDALADSLWGSTQELAPATLVVVGAS
                     "
     gene            complement(42626..43393)
                     /locus_tag="VFF98_06775"
     CDS             complement(42626..43393)
                     /locus_tag="VFF98_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63367.1"
                     /translation="MTAEYAPPRAGASAVLLLFSAGRRADAEAVAQLLASQPGAARHF
                     AISHRPPAEEGWLELLITGLAFDLVGLAPAAALPTPAVRHRFGVAGPDPEAPLEAVVL
                     RPGAHLAGGENLLPVVRAMMQLAVDLAGLGGVEAVIWAPAATMMGRDYFLRNVGRWLE
                     GGAFPALGLTALLREADGAICSDGLAFFTGQELRIEPTSQNGAGGDGKIAIRLIHSLV
                     SGGDISPPCLVDGPEGEVLRIEPARSGGLWRVWRAAG"
     gene            complement(43493..43975)
                     /locus_tag="VFF98_06780"
     CDS             complement(43493..43975)
                     /locus_tag="VFF98_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZU63368.1"
                     /translation="MLLIVWHSRTGASAALARAALAGARGQGAEVRLVAAARARPELL
                     LAAAAYLFVCPENLASMSGAMKEMFDRAYYPLLGRAEGRPYATIIAAGSDGHGAEAQI
                     DRIATGWRLKRVAPPLIINMGAQTPEQICAPKRLSPDQRAQARDLGAALGAGLALGIF
                     "
     gene            44045..>45394
                     /gene="recJ"
                     /locus_tag="VFF98_06785"
     CDS             44045..>45394
                     /gene="recJ"
                     /locus_tag="VFF98_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZU63369.1"
                     /translation="MSIRQAPSPAEPARITHMGQSLTARAWRWRGGNMDIAGDGALAA
                     HTPGEDLVTQLLLARGVAPADLERQRTPTLRAFLPDPSVLQDMDKAAARLADAVVAGE
                     AVAVYGDYDVDGATSAALLLRFLRMLGREGRPYIPDRLLEGYGPTGEALLRLAAEGAQ
                     LILTVDCGAMAHEALAMAADGGVDVIVVDHHKCAAELPRALALVNPNRLDESPEGASF
                     GHLAAVGVAFLLAIATARELRRRGWFAGRQEPDLLALLDLVALGTVADVAALHGLNRA
                     LVAQGLKVMARRENIGMAALADSSRLSRAPTCSDLGFAMGPRVNAGGRVGEAMLGVRL
                     LTSDSPEEAREIAAQLSRHNEERRAIEAAVQQAAEALIDAQHNRSVLVLAGEGWHPGV
                     IGIVAGRIKEKTGKPTFVVALDGAQGKGSGRSVAGVDLGAAVIAAREAGLLVAGGGHA
                     "
BASE COUNT         7619 a        15307 c        15146 g         7322 t
ORIGIN      
        1 tcagcgccga aagcggccac acgctcacct atgccgggct ggaccggctg gccaaccagg
       61 gcgcccacct gctgcgcgcg ctggggctgg cgcggggcga tgtgctggcg gtgatgctcg
      121 acaacgactg cgccgccttt gccatcgcct gggcggcaga gcgcgccggc ctctatctca
      181 ccagcatctc cacccgcctc agcctggccg atgccgccta tatcctggcc gattgcgagg
      241 cgcaggcgct cgtcgtgtcg gacaggctgg ccggctttgg cgccgatgta gcgcgcacac
      301 ggcccggcct gcccgccttc acgctgggcg cgccggccgc cggccttgcc agctggatgg
      361 ccgccatcgc ccccctgccc acaaccccca tcgccgatga aagcagcggc accgacatgc
      421 tctattcctc gggcaccacc gggcggccca agggagtgaa gccggccctg cccaccggcg
      481 atcccggcgc gcccacgccg ctggtggcca tgggccggca gctgtatggc atgggcgagg
      541 agacggtcta tctctccacc tcgccgctct atcacgccgc gccgctgcgc tgggcgatga
      601 cggtgatgcg gctgggcggc accgtcatcg tcatggaaaa attcgacgcc gaggccgccc
      661 tgcgcctgat cgcgcgctac cgggtgaccc atggcacctg ggtgcccacc catttcgtgc
      721 gcctgctggc cctgcccgaa gcggtgcgcc ggcgccatga cctgtcctcg ctggcccgcg
      781 ccatccatgc cggcgcgccc tgcccggtgc cggtgaaaca ggcgatgatc gactggtggg
      841 ggccgatcat cgacgaatat tattccggca cggaaatgtg cggcatcacc gcccttgacg
      901 cgcgcgaatg gctggagcgg ccgggctctg tcggcaaggc ggtgctgggc cggctgcata
      961 tccttgatga cgacgggcgc gaactgccct gcggcacgcc gggcaatgtc tatttttccg
     1021 atggcccggc ctttgcctat caccgggatc cggccaagac cgccgccgcc cacaatgaaa
     1081 agggctgggc aacgctgggc gatatcggca aggtggacga ggcgggctat ctctatctga
     1141 ccgaccgcaa gaatttcatg atcatctcgg ggggcgtcaa catctatccg caggagatcg
     1201 agaactgcct gctgacccat ccgctggtgg ccgatgccgc cgtggtgggc tgccccgacc
     1261 ccgaaatggg cgagtgcgcg gtggcggtga tccagctggc ccccgaagcc gtggccgggc
     1321 cccacaccac cgccgacatc cgcgatttcc tgcgcgcgcg gctgggcggc gtgaaaaccc
     1381 ctcgccgctt cgaatttcgc gccgaactgc cgcgtgagcc caccggcaaa ttgctgaaag
     1441 cggcgctggt ggccgaattt cgcggccgca ccagccagca gccctgacga gaggagccaa
     1501 catgcacatt gccgaagcca ttgccacccg ccgctccgtg cgcgcctatc tggatcgccc
     1561 ggtgccgctg gaaacgatcc gccgggtgct ggatctggcg cgctgggcgc catcgggctg
     1621 caactaccag ccctgggaag ccaccgtgct gaccggcgcg ccgctgcagg cgctgcagga
     1681 aaagctgctg gccgcccagc ccgacgatcc gctggaatat gattgggccg cccccggcaa
     1741 gcatgcccgc tatcaggcgc ggctgcaggc ggtgggcgcg gcgatgtatg gcgcgctggg
     1801 cgttgcccgc gatgcgcccg gccagcgcga ggccttcgcc cgcgccaaca tgacctcgtt
     1861 tggcgcgccg gtgctgctgc tgtgccactt ccccaaattc atgaaagagc cgcaatggtc
     1921 ggatgtcggc atgtggctgc aatcgatcat gctgctactg cgcggcgagg ggctggatag
     1981 ctgcccgcag gaatatatgg gcaattatgg ccgcaccatc aaagcccagc tgggcctttc
     2041 ggacgagacg atgctgttct gcggcctggc catcggctgg cgcgacgagc aagcgccggt
     2101 caacgccttt gcccgcgaac gggtggcgct ggacgatcag gtggccttcc tcggctttga
     2161 cgcggcattg aacgccgcct taccagcctg attgtcggcc tgattgcccc cttgccccgg
     2221 gggcgatctg cccctaggat gggcaacaat aatttacaat tttattccaa atacttaatc
     2281 catcagcagt gcgacgcgac gtggaagaga cggatatatt actgcctgcc cccggcatgc
     2341 gaccggggca acaggaccca ttgcccctcc cttctgccac aaggcgccgc ctgccgggcc
     2401 tggaagcgct gcgctgcgtg gcggcgctat gcgtgctggc gctgcatttg cgggcggtgt
     2461 ttggcgggcg ctatgtgttc ggccggggct atctgggggt ggatttcttc ctgatgctgt
     2521 cgggctatct gatggcccgc acgcaggaag cgcgcctggc cgccggcgcc agcccctggg
     2581 ggtttctgct gcggcgctat ggccggctgt ggccgttgat ggcgctgggc agcctgccgg
     2641 gcgtttgggt gcaggcgctg gcgctgggcg tggtgagcgc ggcggtgtgg tggcgctatg
     2701 ccctcaccgc cacgctcaac ctgctgctgc tgcccgacat gcagacctat ttcgccttcc
     2761 ccatcaacat cccggcgtgg acgatctttt tcgagctggt gatgaacgcc ctgcatgtgc
     2821 tgctgttctg gcggctgcgc gggggctggc tggcgctggt gctggcggcg ctgctgccgg
     2881 tggtggtggg gatggccttg cgcaacaacg ggctggatgt gggcgcccgg ccggaaacct
     2941 tcctgctggg cgggctgcgg gtgggctttg cctatctcat cggcgtggcg ctctatcgtt
     3001 ggtggcgcga tgtgccggtg ctgccggtgc cgccgctgct ggccattgcg ctgatgccgg
     3061 cgctgctgcc gctgctgttt gccctgcgca tctggagctg gtggcccgat gtggcctttg
     3121 tgctgctggt gggcccgctg atgatcgccg gcgcgctgcg cctggcgcca caaggggcac
     3181 cggcccgctg gtccacgctg gccggccagc tggcatttcc gctgtttgcc ttccagatgc
     3241 cgatcctgca aggcttccac gatcagagct ggggctattg gccggcggcc gcagcggtgc
     3301 tggcggtggg cgtgagcggc gcctgcatcc tggcatggct ggccgcaaga aagggaaccg
     3361 ccgcatgaca gagcctgtgt tgatcacccg cgaggaaggc ggcatcaccc ttctcacgct
     3421 gaaccgcccg gccctgcgca accccatttc cgaccccgac atgctggagg cgctgctggc
     3481 ggcgctggcc gatgccgatg ccgatccggc cacgcgcgtc atcatcctca ccggcgccgg
     3541 cggggccttt tccagcggcg gcaatcttaa cgccatgcgc cccggcgccg ggctgatcga
     3601 tgcagcgccg gtgggcacgc ggcagaacta tcgccatggc atccaccgcc tgccgctgct
     3661 gatggcgcgg ctggaagtgc cggtgattgc cgccgttgcg ggcccggcgg cgggcgcggg
     3721 ctgcgatctg gcctgcatgg cggatctgcg catcgccggc caaagcgcgc tgttcatcga
     3781 aagcttcgtc aagatcgggc tgatctcggg cgatggcggg gcctggctgc tgccgcgcgt
     3841 ggtgggcttt gccaaggcgg ccgaaatggc gttgacgggc gatccggtgg atgcgcccac
     3901 ggcgctggcc tgcggccttg tcagccaggt ggtgcccgat gccgatctgc cgcaggcggc
     3961 cctggcgctg gcccggcgca ttgccgccaa tccgccccat gccgtgcgga tgaccaagcg
     4021 cctgctgtgg gaagcgcggc gggcagacct ggccaccatc cttgaaatgg ccggcgccat
     4081 gcaatcgctg gcccatgcga ctaacgacca tgccgaggcg gtggatgcct ttctggccaa
     4141 gcggcctgcc cgatttacgg gctcgtgatg tgatctgaat gtgatatatt gccaccatca
     4201 cattcctgtg tgatgaggcc catcatgagc aaccgaatca tcgaacaggt ccgccagctg
     4261 gtggccgatg gacagctgac caaggccggc atcgcccgcg ccgccggtct tcacgccaac
     4321 acgctgcgcg attgcaccga gccggactgg aaccccaccg ccgaaaccct cgccaagctg
     4381 gagcgctttc tggccgagac ggacgagcgg ccggtgctgg tcggcatcga ggagatcatc
     4441 gaggaagcgc gcaacgggcg catgtatatc ctggtggacg acgaggaccg cgagaatgag
     4501 ggcgacctca tcatccccgc gcagatggcc acgccccagg cgatcaactt catggccacc
     4561 catggccggg gcctggtctg cctggccctg tcggccgagc gggtggagca gctggggctg
     4621 gagccgatga gccggcgcaa cggcactccg ctgggcaccg ccttcaccgt ttccatcgag
     4681 gccaaggaag gcatcaccac cggcatttcc gcaggcgacc gggcgcgcac cgtgtccgtc
     4741 gccatcgacg gcagcaaggg gcaggccgat atcgtcagcc cgggccatgt gtttccgctg
     4801 cgcgcgcggc cgggcggcgt gctggtgcgc gccgggcaca cggaggcggc ggtggatatc
     4861 tcgcggctgg ccgggctcaa cccctcgggc gtgatctgcg aggtgatgcg cgaggatggc
     4921 accatggccc ggctcgacga cctcatcggc tttgcccgcc gccatggact gaagatcggc
     4981 accatccgcg atctcatcgc ctatcgccgc cagcatgacc gcatggtgga gctgagccgc
     5041 gagatcacct tcaccagccg gcatggcggc gaatggctgg cccgttccta tcgcaaccgc
     5101 gccaccggcg aggacaatct ggcgctggtg aagggccgca tcgaccccga ccagccaacg
     5161 ctggtgcgca tgcatgtgct ctcggtcttc tccgacattc tgggcgaggt gagcgagcgg
     5221 gccgaactgc tgcaccgctc gatggagatc atcgccgagg cgggcgcggg cgtgatcgtg
     5281 ctgatcaaca agccgctgcc gcgttccttc tcccgcgcga tcgagatcaa ggagcagctg
     5341 cgcaccggca acacgcaagc catggaagag ctgcgcgact atggcgtggg cgcgcagatt
     5401 ctgacagagc tgggcgtgca tgacatgatc ctgctcacca acacttccca cacgctcgtc
     5461 gcgctcgaag gctatggcct gtcgatctcg ggcgaacagc gcatcgattg aaggcacctc
     5521 atcccatggc caaattcctc attgtcgaag cccgcttcta cgaccacctg aacgacatgc
     5581 tggtggccgg cgccaaagcg gcgctgaagg cggccggcca caaggccgat gtcatcaccg
     5641 tgcccggcgc gctggagatc cccggggcca ttgccctggc cgatcaggcg ggcgaatatg
     5701 atggctatgt ggcgatcggc gtggtgatcc gcggcgagac ctaccatttc gagatcgttg
     5761 ccggcgaaag cgcgcgcggc gtgatggcgc tgtcgatgga cgggctggcg atcggcaacg
     5821 gcatcctcac ggtggagaac gaggcgcagg cgatcgtgcg cgcagacccc gcccagaaag
     5881 acaagggcgg cgaggcggcc aaggcggcgc ttgccctgct tgccctgcgc gagcgtttcg
     5941 gcgcctgatc ctgcgccccc ggcgggggtg tgcgacagcc agaaggccgg ctcccccagc
     6001 gggagccggc ctttttgcat ggggctgccc ggcatgggcc atgatgcgcg attcggcccg
     6061 ggcggggcca aattacgggc tttttcggag gcagcgctaa gcgttaaccg atattcatgc
     6121 ctgctactct ggctttatcg gcaaaaataa gtattattgc ttgtaccgta aatacaacaa
     6181 ccaggacagg aaatcggcga tgaacgatct ctctctcccc tccgcagccc ccatgccccg
     6241 tctcgcatct gcggtatttc ccgaaagaaa catggttttc aacacgctta cagactgcct
     6301 gaaccgtctg gatatgctgg gagagcagct ggccgcggcg catctggcgg cttgcctcga
     6361 ttccctgcgt gaaacattca ttatggatgg aaacatatcc gatatggata tacaccgccg
     6421 ccgccgcaac cttgacatag ccaaatcgga ctaaatatta ttacacatgt cgccgttctt
     6481 cgatgaacga gaaaacagcg aagaatttta tgtagttgta ctagaattgt attattttca
     6541 cccttgtttc aaagcgggac gccatgacac aagagatcgc cagcagctac acttccaccg
     6601 aagtggccac ccaacttctg gggatcaccc agtctctcga tgtgcttgcc agccaacttc
     6661 attccattgc gggacaaaat cggcccgtac tgaagcaggc cagcgcacgc cgggaactgc
     6721 ccccctcggc agaacaactg gccaagatcg ccggccaggc ctatcgggac cggcggcggc
     6781 gcaccgaatt gtttggcgat gaaacgctgt ttggcgagcc ggcgtgggat attctgcttg
     6841 atctgttcgt ggcggccatc aagcacaagc gggtggcggt caccagcgcc tgcatcggct
     6901 ctgccgtgcc caccaccacg gcgctgcgct ggctgaagat cctggaggag aagggcctga
     6961 tcctgcgcga ggaagacccg gacgatgccc gccgcacctt cctgcgcctg tcgcccggcg
     7021 cctacgacct gatggtcgat tatttccacc ccgaacagaa ataaccggcg agaacccccg
     7081 ccggccattc ctgtatacct gaaacggggg cgccatggcc cccgctgacg gatcagcgca
     7141 gcaggcgccc gaaggccgcc ttgccggcat agcgcgacgc cgcgcccagc tgctcctcaa
     7201 tgcggatcag ctggttatac ttggcaagcc ggtccgaacg ggccagcgag ccggtcttga
     7261 tctggccgca gttggtggca acggccaagt cggcgatggt cgcatcctcg gtctcgcccg
     7321 aacggtggct catcaccgcg gtgtagccgg cgcgctgggc aatgctcacc gcttccagcg
     7381 tctcggtcag gctgccgatc tggttcacct tcaccagcag cgaattggcc aggccctgct
     7441 cgatgcccat ggtgaggcgc ttggggttgg tgacgaacag atcgtcgccc accagctgca
     7501 ccttgtcgcc gatgaggtcg gtcagcgcct tccagccctc gaaatcatcc tcgctcatgc
     7561 catcctcgat cgagatgatc ggatagtcgg ccgccagcct ggcgagatat tcggcaaatt
     7621 ccaccggcga gagcgacagg ccttcgcccg agatctcata cttgccgttc ttgaagaact
     7681 cggtcgaggc gcaatccagc gccagcagga tatcggtgcc cagcttgaag ccggccttct
     7741 cgatcgaggc cgagacgaaa tcgagcgcag cgcgggtgct ggcgatgttg ggggcaaagc
     7801 cgccctcatc gccaacggcc gtcgccaggc ccttctcgtg caggcccttc ttcagcgtgt
     7861 ggaagatttc cgcgccccag cgcacgccct cggcgatgct gtcggcgccc accggcatga
     7921 tcatgaattc ctggaaatcg atcgggttgt cggcatgctc gccgccgttg atgatgttca
     7981 tcatcggcac cggcagcaca tgcgcggcaa cgccgcccac ataggcatac agcggcaggc
     8041 cgcgcgcatc ggcagcggcc ttggcgatgg ccaagctggt gcccagaatg gcgttggcgc
     8101 cgatgcggcc cttgttctcg gtgccgtcca gctcgatcat ggcgaggtcg atatcgcgct
     8161 ggtcctcggc gtcgaggccg acgagcgctt cgtggatctc ggtgttcacc gcttccaccg
     8221 ccttcagcac gcccttgccc agatagcgcg ccttgtcgcc atcgcgcagc tccaccgcct
     8281 catgcgcgcc ggtgctggcg cccgagggca cggcggcgcg gccgaagctg ccatcttcca
     8341 gcagcacatc gacctcaacc gtgggattgc cgcggctgtc gaggatttcg cgggcgtgga
     8401 tatcgattat cgcggtcatg gcgggttcat ctcctctcgg ccagaaatcg gggccatcgg
     8461 ggccgctctt agcaggcacg ccttggcagg caagccgcat tgcagcaata tgcggttggc
     8521 cataggaagg aagccggagg cctgctattg tgcagctgcg agatatcggc catggccgat
     8581 gccaaaccgt tgaacctctt gcgcgaaagg gacatgccat gtccgagccc gaaaccatca
     8641 ccgctgtgac cgacgccgtt gccgttgcaa gccctgcgga agggcccgca gaaggcgatg
     8701 ccaaggcgcg cttctccaag gcgctggacg aggcgcgcgc gggcgctgtg gcgctgggca
     8761 aggatgtgca gggcaaggcc ggcgcctatg ccgacaggct gggggccaag ggccacgacc
     8821 tgctcgacga tgccaaggcg ctgaccgagg aagcgaagga ccgcgccgcc accctggccg
     8881 acgaaggcaa ggtgcgggca agcgaggcgc tggtgagcct gggcaagctg gtggaagaaa
     8941 acgccgccct ggtggaagag aagctgggcg gcaaatatgg cgagtatttc cgcaccgcgg
     9001 cccagcacat caacgatgcc gccgccagga tcgaggccaa ggatctgggc gagctgggcg
     9061 aggatgcccg taccttcgtg cgcgagaagc cggcgctggc ggtgggcctt gcggcggcgg
     9121 gcggcttcgt gctggcaagg ctgttcggcg gcaagtcctc gaagtaatcc aagcgcctgc
     9181 ccctcctcag gaacacgaga tgcgcaacct gttcagcgcg cgcccgccgc atgatgcgga
     9241 agagggcgag ccccatcccg gcagccaggc tgatgccggg gccgatgccg gggaagacag
     9301 ccttggcgcg ctgctgcgcg gcctgatcgc cgccctcggc cagctgtggc aggccgagat
     9361 cgcctatcag cgcgcccgcg cccgggcgct ggccggcatg atcggcatga tcgctgcctt
     9421 gctggtggcg gcggtgatat tggcgctggc cttgctggtg gcggtatcgg tggggctggt
     9481 ctgggcgctc acggccaggc tgggggcgtg gggggcgatg ggcgcggtgg cgctgggcct
     9541 gctgctgctg ctggcgctgg tgctggtgct ggccgggcgc atgggccggc gcatcgcccg
     9601 ggtgatcttc ggtggaggcc tgcgcggatg agccggctgg aaaaggacct tgccgccgcc
     9661 cgcgccgcgc gcgatgcggc ccaggcagag ctgacggcca ggctgggcca ggcccgcgcg
     9721 gccgtgcatc cggcgcgcct tgccggccgg ctgaagcgcg aggccggcgc ccgcgcgcag
     9781 gatatcgccg ggcaggcgct ggaaatcgcc accgatcatc gcggcatact ggtcgccacc
     9841 gtgtcggcca cgctgatgtg gctggggcgc aagcagatcg gcaagctggc cacaaggcat
     9901 ctgccggtgg aacgctatgc ggcgctggcc aaatcgctgg ggccgcaggc gttgcagggc
     9961 gcccggggga agcggctggc gcggcaggtg tcgctgtggg ggccgcgggc ctggcgcttg
    10021 tggaagcgcc tgcgcctgcc cggcgcgcgc taggggcagt tgcgcaaggc tggcggaaaa
    10081 ccgcagtttt tgcaccaaag acctgacaga aaagacaggg taatgcttgc cgttcgcaag
    10141 ccgccggtgc tatggcgaag cctcctcttc ccagacatgg cgactgatat gcaaaaacta
    10201 catctggtta tgggcgggcg cgtggcggac ccgcaagggc ttgaattcgt ggatctgaag
    10261 gcgctcgatc tggtcggcgt tttccccgat tacgcggccg ctgtggaagc ctggcgcggc
    10321 gctgcccagc gcagcgtgga tgatgccgag atgaaatacg tcatcgtgca tctgcacaag
    10381 ctgctcgagc cggacgccga ataagccggc cgacctagct gaaatgacaa aggccccgcc
    10441 aaggcggggc ctttctgtta agggcaagct gcctgcgcgc gagcgcggca gcccctgccc
    10501 tcagatgaat tcgatcttct cgaccacgta gaagcgctcg ccggccggca cgctcacttc
    10561 gatctcatcc tcgatgcgct tgccgatcag cgcccggccc agcggcgaat tatagctgat
    10621 gcggcccacc ttggcatcgg cctcataggg gccaacgatc tgatagcgca ccggcttgtc
    10681 gtcgtcgtcg agcagggtca cggtcgcgcc gaacaccacc ctgtcgcccg aaagcgtggt
    10741 ggggtcgatg atctgcgcgc ggcccacctt gtcttccaga tcgccgatcg aggcttccac
    10801 ctggccctgg cgctccttgg cggcatgata ttcggcattt tcggaaagat cgccatgcgc
    10861 gcgcgcttcc tcgatcgcct cgacaatctt cggccgttcc tcgcgcagca ccttcagctc
    10921 ggcgatcagc ttttcatagc cttccgccag catcggcagc ttttccacac tcgccatcgt
    10981 gtcttttgtc ctcctggcct tgccttaagg ctgcggcccc ctcgcccctt cttgcggcaa
    11041 tccgcaagaa gggctgggaa accgaccgca gctgtgggga aggcgtgatc gttcagttat
    11101 aataagcctg caacggagac acttcaagac tggtcgcccg cagggcggca atcgcctggg
    11161 cggcggccac gctggcgctg gcagtggtga aatagggcac cttgccggcc agcgccgcct
    11221 gacggatgct ttgcgaatcc ttcaggctct gccagccctc ggtggtgttg accaccagcg
    11281 ccacatcgcc atcgatgatc cggtcaacga tatgcgggcg cccttcggcc accttgttca
    11341 cctgctgcac cggcacgccc tcggccgcca ggaactttgc cgtgccgccg gtggccacca
    11401 gctggaagcc cagctccacc agctggcgcg cggccggcag gatcaccgcc ttgtccgtgt
    11461 ccttcaccga gaggaaggcc acgcccccct gcggcagctt ggtgccggcg cccagctggg
    11521 ccttggcaaa ggcggtggca aagcccttgt cgatgcccat cacctcgccg gtggacttca
    11581 tctcggggct gagcaccgga tcgacaccag ggaagcgcgc gaaggggaac accgcctcct
    11641 tcaccgccat atagggcaga tcgcgcttga acggcgggaa gctggccagc ttttcgcccg
    11701 ccatcacccg cgaggcgatc ttggccaccg gctggccgat ggccttggcg acaaacggca
    11761 cggtgcggct ggcgcgcggg ttcacctcga tgaggtagac ttcgccctcc ttcaccgcga
    11821 actggatgtt catcagcccc ttcacgccca gcgccagcgc cagcgcgcgc gcctggcgct
    11881 ccatctcggc gatgatgtcg gcgcccagcg aatagggcgg cagggtgcag gcgctgtcgc
    11941 ccgagtggat gcccgcttcc tcgatgtgct gcatcacgcc ggcgacgacg acatcctcgc
    12001 catcgcacag cgcatcgaca tcgcattcga tcgcatcgcg cagatactgg tcgatcagca
    12061 ccgggctgtc gcccgagacc tgcaccgccg tggcgatata atcgtccagc tgcgccacgc
    12121 tgtcgacgat ttccatcgcc cggccgccca gcacgtaaga ggggcgcatc agcaccggga
    12181 agccgatgcg attggccacc gccaccgcct cgtcgcggct gcgggcaatg ccgttggcag
    12241 gctggctgag gcccagcttc tccaccagat cggcaaagcg ctcgcggtct tcggccagat
    12301 cgatggcatc ggggctggtg cccagaatgg ggatgccggc atcttccagc gcctgcgcca
    12361 gcttcagcgg ggtctgcccg ccaaactgca cgatcacgcc caccagatcg cccttcgact
    12421 gctcgacgcg caggatctcc agcacgtcct cggcggtcag cggctcgaaa tagaggcggt
    12481 ccgaggtgtc atagtcggtc gacaccgtct ccgggttgca gttgaccatg atcgtttcat
    12541 agccggcatc ggccagcgcg aaacaggcat ggcagcagca atagtcgaac tcgatgccct
    12601 ggccgatgcg gttgggcccg ccgcccagga tcacgatctt cctgcgctcg gaaggttcgg
    12661 cctcattctc ggcctcgccg aacagcgggg cctcataggt cgaatacatg tagggcgtca
    12721 ccgcctcgaa ctcggccgcg cagctgtcga tgcgcttgaa caccggatgc acgcccagct
    12781 tgtggcgcag cgcgcgcact tcggcctcgc tggtggcgcc ggccatggct tccaccacat
    12841 catgcaccag ccctgcgcgc gccacattgg cgctgcccac catatgcacc gattccaccg
    12901 ccagcttggc cagccgcttg tccgagaagc ccatggcctt caggcgccgg aggccggcgg
    12961 catcattggg caggccatcg gcgatgatct tggcttcctc ggcgatgatc tcggcgatct
    13021 ggcgcaggaa ccacgggtcg taatgggtga tggcgtggac atcgtccacg ctcagccccg
    13081 cgcgcagggc ctgggccacc accagcaggc gatcgggcgt ggcgcgcgaa agggcagcgg
    13141 taatctcgtc gcggctggcg cccaccagat gatcgacgcg gttgaagcca tcaaggccgg
    13201 tttccagccc gcgcagggcc ttctgcatcg attccttgat gttgcggcca atcgccatca
    13261 cctcgcccac gctcttcatg gcggtggaga ggatcggctc ggcgcccttg aacttttcaa
    13321 aggcgaagcg cgggatcttc gtcaccacat agtcgatggt gggctcgaag gaggccggcg
    13381 ttgcgccggt gatctcgttg gtgatctcgt ccagcgtgta gccgatggcc agctttgccg
    13441 ccacgcgggc gatggggaag cccgtcgcct tcgaggccag cgccgaggag cgcgacacgc
    13501 gcgggttcat ctcgatgacg atcaggcggc catcggccgg gttcaccgcg aactgcacgt
    13561 tggagccgcc ggtttccacg ccgatctcgc gcagcaccgc gaggctggcg ttgcgcatga
    13621 tctgatattc cttgtcggtc agcgtgaggg ccggcgcgac agtgatggaa tcgcccgtgt
    13681 ggacgcccat cggatcgaca ttctcgatcg agcagatgat gatggaattg tcgtggcggt
    13741 cgcgcacgac ctccatctca tattccttcc agccgagcag cgattcctcg atcagcacct
    13801 cggtggtggg gctggcttcc aggcccgaac gcacgatctt ctcgaactcg gccttgttgt
    13861 aggccacgcc gccgccagtg ccgcccagcg tgaaggaggg gcggatgatc gagggcaggc
    13921 ccgtcgtctc cagcaccgcg aaggcttcct ccaccgtgtg cgccacgccg ctgcgcgcgc
    13981 tttccaggcc gatcttgtcc atcgcctcgc ggaagcgctg gcgatcctcg gccttgtcga
    14041 tggcatcggc atcagcgccg atcatctgca cgccaaattt ggccagcgtg ccatcgttga
    14101 agagcgcaag cgcggtgttc agcgcggtct gcccgcccat ggtgggcagc agcgcatcgg
    14161 ggcgctcctt ctcgatgatc ttggcgacga tctcgggggt gatcggctcg atataggtgg
    14221 catcggccat ttccggatcg gtcatgatcg tggccgggtt cgagttcacc aggatcaccc
    14281 ggtagccctc ttccttcagc gccttgatcg cctgcgtgcc cgaatagtcg aactcgcaag
    14341 cctggccgat gatgatgggc cccgcaccga tgacgaggat cgaggagatg tcagtgcgct
    14401 tgggcatcag gcgaggctct caatgaattt cgtgaacagg tagaggctgt cttgcgggcc
    14461 gggcgaggct tcggggtgat attgcacgct gaaggcctgc ttgccggcga tttccaggcc
    14521 gcaattgctg ccatcgaaaa gcgacacatg cgtttcgaca acccctgccg gcaggctggc
    14581 ggcatccacc gcgaagccgt ggttcatgct ggtgatttcc accacgccct cggccaggcg
    14641 cttcaccggg tggttggcgc cgcggtggcc ctgatgcatc ttgatggtct tgccgccggc
    14701 ggccagcgcc agcaactggt ggccaaggca gatgccgaag atcggcacgc cggcatcaag
    14761 cagcgcgcgg atcaccggca ccgcataaac gccggtggcc gccggatcgc ccgggccgtt
    14821 cgacaggaac acgcccgccg gcttcagcgc cagaatggcc tcaaggccgg tttcggccgg
    14881 caccacggtg acgcgcgcgc cagcggccac cagattgcgg aagatattgt ccttcgcgcc
    14941 gaaatccatg gccaccacat gcggccggcc atcctcggcc ggctgggcat agccctcgcc
    15001 cagcgcccag gtgccgccgt gccagtcctc tgcgccctcg cgggtgacga ccttggccag
    15061 atccatgcct tccagccccg gccaggcgcg cgccttggcg agcagcgcgg ggatgtcgaa
    15121 attgccgacc ggattatgcg cgatcaccgc attgggcgcg cccgacaggc ggatgcggcg
    15181 ggtgagcgcg cgggtgtcga tgccggcgat gccgatcttg ccccgggctt ccagccaggc
    15241 ggaaaaatcg ctctcagcgc ggaagttgga cggggcagtc acatcctcgc gcaccacgca
    15301 gccaaccgcg ccatcgacat ggctctccag gtcttcctcg ttcacgccga cattgccgat
    15361 gtgcgggaag gtgaaggtga cgatctggcc ggcataggag ggatcggtca tgatctcctg
    15421 atagccggtg atggcggtgt taaagcacac ctcgcccacc gcgctgccga ccgcgccaaa
    15481 gccgcggccc caggcgacat gcccatcggc tagcaccaga acagcggttg ctcccgcagg
    15541 tttgggcgca agcaaagggg cggggtctac catggaaagg cgctccgttt ggaagggttg
    15601 acagatatgt gtgctaaggc ccgcccgcta gaccccatgc ccctgcccgt caacccctgg
    15661 cgggggataa ttgccgggac aggtggacac agggagcccc cctcgcctag gcagcccggc
    15721 caattgccgc tagtctgtaa cgctttcggc gcgcggcgcc tcatcatcca cgggaacgaa
    15781 aaaatgatcc gcgacaccat caaggccgcg caggtggctg ccatgaaatc gggcgacaag
    15841 gcccgccttg ccgccgtgcg gctgatcctg gccaagctga aggacaagga catcgaactg
    15901 cgcaccgccg ccagcctgcc cgacgacgat acgctggtga ccgacgtgct gcagaagatg
    15961 atgaagcagc ggcgcgaatc catcgccatg tatgaacagg gcggccgccc cgaactggcc
    16021 gcggcagaag cggcagaagt ggccgtgatc gaggatttcc tgcccgcgca gatgggcgag
    16081 gacgaagtga aggccgcgat cgaggcgatc aaggccgagc tgggcgcggc cggccccaag
    16141 gacatgggcc gcgtgatcgc cgagctgaag gcccgccacg gcacggtgat cgacatgggc
    16201 aaggccagcg cgctggtgaa ggccgcgctg gcgtaaaccc gatggcgctc acgccccaat
    16261 ggctggatga actgcgcagc cgcatcacgc tgtcgggcgt gatcgggcgg acgacgcgca
    16321 tccagaaggc ggggcgcgag ttcaaggcct gctgcccgtt ccacaacgag aagtcccctt
    16381 ccttcaccat caacgacgag aaggggttct atcactgttt cggctgcggc gcgcatggcg
    16441 atgtcatccg ctggatgacc gaccagcgcg gcctcagctt catggatgcg gtgaagcagc
    16501 tggccgagga agcgggcatg gaagtgcccg ccgccgatcc gcacaccgcc cgcctctcgg
    16561 cccagcgcgc cgaacaggcc aagaccctgc acgatgtgat ggccgccgcg caggactggt
    16621 ttgccgccaa tctggccggg cccgaagggg cagaggcgcg cgcctatctg gccacgcgcg
    16681 gcttttcggc agaggtgtgc cggcagttcg gctttggcta tgcgccggaa gggcgccagg
    16741 cgctgaaggg tgcgctcgac cgctttcccg aggcgatgct ggtggaagcg ggcctgcgca
    16801 tcgcggtgga agagcgcgag ccctacgacc gctttcgcgg ccggctgatg ctgcccatcc
    16861 aggatgcgcg cgggcgggtg atcgcctttg gcgggcgcat cctcagcgcc cagaagaccg
    16921 acgcgcccaa atatctcaat tcgcccgaca cgccgctgtt cgacaagggc cgcacgcttt
    16981 acaacctgca ccgcgccgcc cccattgccc gccagagcgg gcggctgctg gtggtggaag
    17041 gctatatgga tgccattgcc ctgtcggccg cgagctttgg cgattgcgtg gcgccgctgg
    17101 gcacggcgct gacggaaacc cagatcgagc tgctgtggcg ccaggtgccc gcccccatcc
    17161 tctgcttcga tggcgacaag gccggcctgc gcgccgctgc caaggcgatg gtgcgcgcgc
    17221 tgccgctgct gcggccgggc cattcgctgc gcgtgctcag cctgccggcg gggcaggatc
    17281 ccgacgaatt gctgaaggcg cgcggccccc aggcgatgga cgcgctgctg gaaggcgcgc
    17341 aagggctggc cgacgccctc tggcagcatg agcgcgatgc cctgccgctg gccggccctg
    17401 aagacaaggc cgggctgaag gcgcggctga tggcccatgt cgatgccatt gcccaccccg
    17461 atatcaaggc gctttatcgc cgcgagtggc tggatcgcta tggcgaattc gcctttgccc
    17521 ggcccgagcg gcccgcccct gccccccgcc cgcaggcgcg cagcggtggc ggcaggcgcg
    17581 gatcctggcg cgatgcgccg ccccctgcgc ccttgcccga aaccgcggcg cgcctcgccc
    17641 ggccggcggc cagcgtggcc gggcgggcgc gggcgatcac catggtggtg ctggggctga
    17701 tgcgcaatcc ggccgcgctg gcccggctgg cagagccgct ggcccggctg gcgcctgaag
    17761 agccgcggat cgatgcgctg ctcgatgcgc tggattacgg cctgcccctt gaaggcgccg
    17821 atatcgccac catattcgcc gggcgggcgc tggcagaacc ttcccttccc ggcccgcgcg
    17881 aggcggtgtt cgccttcgtt gccgaaagcg gcgaggcggc catggcagag ctggccgagg
    17941 tggtggcggt gctggtggaa cgccccgcga tcgaggaggc actggcccag gcccaggccc
    18001 gcttcgaggc ggaactgacc gaggaggccg gcgaggaagt gacccggctg cgcacgcttt
    18061 tgttggcgtt gcagaaaaga gagctggatt tgaacgcgcg actcggccaa atggcaacag
    18121 gcaatgccga tcgcgcaaca ggcacaggcc ccgaagccgc cggcgcggat gcgggcaaca
    18181 aaatggcgga atgatggacg aagagatttc tggcacggac atgggcggcg tggacaaggg
    18241 cgaaggcggc gatgcaccgc tgatcgacct gaacgaagcc tcgatcaaga agctgatcgc
    18301 ccgcgccaag cgccgcggta tcatcaccta tgacgagctg aacgaggccc tgccgcagga
    18361 ccagatgtct tccgagcaga tcgaggacat catggccgcg atctcggaaa tgggcgtcaa
    18421 catcgtcgag agcgacgagg acaatgccga gcccgacgat gccggcaatg acgatgtcga
    18481 agattccgat gccatggtct cggaccggcc agcgcccgag aagaagcgcg agacgattga
    18541 gcgcaccgat gatccggtgc gcatgtatct gcgcgagatg ggcgcggtcg agctgctgag
    18601 ccgcgagggc gagatcgcca tcgccaagcg catcgaggcc ggccgcgaca cgatgatcct
    18661 gggcctgtgc gaaagcccga tcaccttcca cgcgatcatc cagtggtccg aagcgctcaa
    18721 caatggcgag atgcagctgc gcgagatcct cgatctcgat gccatgctga gcaaggaccc
    18781 ggcgcccgag aacctctccg aagacggcga ggacgatggc gacggcgaga tttccgaggc
    18841 caccgccggc ccctcctaca aggaggaaga ggatatcgag gaggaagaat cggccgatgg
    18901 cgaggatgag gacggcatcg agcgccgcgc ccgcccgccg gccgaggacg acgaggacga
    18961 caacaccctc tcgctggccc agatggaagc ggcgctgaag cccgaggcgc tggagaaatt
    19021 cgcccagatc accgagctgt tccacgcttt cgagcgcatc caggccgagc ggctggaagc
    19081 gatggcgatc ggcaatgatt ttgccgccga caaggagcgc cagtatcagc agctgcgcga
    19141 ggacctgacc gcgcaggtgg aaagcgtgca gttccacgcc agcaagatcg aatatctggt
    19201 cgacaacctc tatgccttca accgccggct gaccacgctg ggcggccaga tgctgcgcct
    19261 ggccgagcgc cacaaggtga gccgcaagga cttcctcgac gcctatgtgg gccgcgagct
    19321 ggacgatgcc tggctgacct cggtggccgg caaggacaag aagtggggca atttcgctgc
    19381 caatgagggc gcctcggtgg agcgtatccg cgccgaggtg tcggacattg ccagccagac
    19441 cggcatggcc ctgcccgagt tccgccgcat cgtgaacatg gtgcagaagg gcgagcgcga
    19501 ggcgcgcatt gccaagaagg agatggtgga ggccaatctg cgcctggtga tctccatcgc
    19561 caagaaatac accaaccggg gcctgcaatt ccttgatctc attcaggaag gcaacatcgg
    19621 cctgatgaag gcggtcgaca agttcgaata tcgccgtggc tacaagttca gcacctacgc
    19681 cacctggtgg atccgtcagg ccatcactcg ctcgatcgcc gatcaggcgc gcaccatccg
    19741 tatcccggtg catatgatcg agacgatcaa caagctggtg cgcaccagcc gccagttcct
    19801 ccacgagcag ggccgcgagc ccacgcccga ggaaatggcc gagcgcctct ccatgccttt
    19861 ggagaaggtg cgcaaggtga tgaagatcgc caaggagccg atcagcctcg aaacgccgat
    19921 cggcgacgag gaagatagcc atctgggcga tttcatcgag gacaagaacg cgatcatccc
    19981 ggtggatgcc gcgatccagt cgaacctgaa ggaaaccgtc acccgcgttc tggccagcct
    20041 gacgccgcgt gaggagcgcg tgctgcgcat gcgcttcggc atcggcatga acaccgatca
    20101 cacgctggaa gaagtcggcc agcagttctc ggtgacgcgt gaacgtatcc gccagatcga
    20161 ggccaaggcg ctgcgcaagc tgaagcaccc cagccgcagc cgcaagatgc gcagcttcct
    20221 cgaccagtaa tccccccggc agcccggctg cccccatatg aaggcggtat ttccatgcgc
    20281 acgtctgctt cgcgccttgc ggccctgatc gctgtggctg cggccaccat tgcaacgccc
    20341 gtgctggcca aggcggccaa gcccgcgccg gccccgcagg ccagccctgc cgccgccgct
    20401 ccggcccagc ctgccgcccc ggcgctgccg ccggtgccgg ccgcctcgct ggacctcgcg
    20461 cgcaagatcg tggccatctt cttcccggcc gatcagcagg aagcggtggt gcgcaattcg
    20521 gtggccgggc tgatgccgcg ctatcgcgcc gcggttgccc tgcccaaggg gtgggacgat
    20581 ccggcgctta aggcgatggt ggaggccagc tatccgcaga tgcagcagaa gctgcagccg
    20641 atcgtggccg ccggcctgcc gcacctctat gacgcgctgg ccgaggccta tgcgcgcgag
    20701 ttcacgaccg ccgagctgac cgatatcgtt gccttctcgg ccacgccggc gggcaggcac
    20761 tatgtgctgg cgagcgggcg gatcaatggt tatccgatgg tggtggccgc caacaacgcc
    20821 acgctgaccg aggccgccaa ggccgaacag cagcagaccc agctggtggt ggccgaggtg
    20881 aaggactatc tcgccaagca cccggatgtg atggcccgca tcaaggccca ggccgccgcc
    20941 aagccggctg ccgcgcccca tccgcaggcc gccggcacta ccgagcgctg agcaatcccc
    21001 tggccagggg cacctcgcct ctggccagat gcgccgacac gacctatggg ggcggcttgc
    21061 gattcacccc caagagattg taggcgcacc ggcccatgac ccagacaccc gacaccaccg
    21121 ccccgctgac gatcgcgctg gcatccgatc atgccgccgt ggccctgaag gccgagctgg
    21181 tcgcctattt ggcgcagctt ggccatgcgc ccatcgatct ggggccggcc gatgacagcc
    21241 gggtggatta tcccgactat ggctacaagc tggcagaagc ggtggcctcg ggcgtggcgc
    21301 gctttggcgt ggcgctgtgc ggcagcggca ttggcatttc catcgccgtc aaccgccacc
    21361 cggcgctgcg ctgcgcgctg gtgagcgagc cgctctcggc cgcccttgcg cgtgagcaca
    21421 acaatgccaa cgccattgcc atgggcgcgc gcctcaccgg gcccgacatg gccaaggcct
    21481 gcctcgatgc cttcctttcc gcagaattcg gcggcggccg ccatgaaggc cgcgtcgaaa
    21541 aactgtccaa ccccgcgttc tgaaccagcc gaaggagacc ccacccatga ccgcaaccac
    21601 ggatgtgcgc ccgcagggct tcttcaccgg cacgctggcc gagcgtgacc cggccattgc
    21661 cggctggatc aacaaggagc tgggccgcca gcgcgacaag atcgagctga tcgccagcga
    21721 gaacatctgc tccaccgccg tgctggaagc ggccggctcg atcctcacca acaaatatgc
    21781 cgagggctat cccggcaagc gctactatgg cggctgcgaa tatgtcgacg agatcgaaac
    21841 gatcgccatc gagcgcgcca agcagctgtt tggcagccag ttcgccaatg tgcagcccaa
    21901 ttcgggcagc cagatgaacc aggcggtgtt cctggcgctg ctgcagccgg gcgacacctt
    21961 catgggcctc gatctcaatt cgggcgggca cctcacccat ggctcgccgg tgaacatgag
    22021 cggcaagtgg ttccaccccg tgccctatgg cgtgcgcgcc gacgatcacc gcatcgatat
    22081 ggacgaagtg gcccgcatcg cccgcgaatg cagccccaag ctgatcatct gcggcggcac
    22141 ggcctattcg cgcgagtggg actgggcgcg cttccgcgag atcgctgacg aggtgggcgc
    22201 ctatctgctg gccgacatga gccacatctc gggcctggtg gccggcggcg tgcacccctc
    22261 gccggtgccc catgcccatg tgaccaccac caccacccac aagagcctgc gtgggccccg
    22321 ttcgggcatc atcctctcga atgacgaggc gatcgccaag aagctgaaca gcgccatctt
    22381 cccggggctg cagggcgggc cgctgatgca catcatcgcc gccaaggcgg tggccctggc
    22441 cgaggcgctg acgccggaat tcaaggccta tgccgcccag gtgaaggcca atgcggccgc
    22501 ccttgccgcc agcctgcagg ccgaagggct gggcatcgtc tcgggcggca ccgacaacca
    22561 cctgatgctg gtggacctga ccccctatgg cgccaagggc cgccatgccg agcatgcgct
    22621 cgaccgggcc agcatcacct gcaacaagaa ctcgatcccc ttcgacccag aaaagccgat
    22681 gctgacctcg ggcattcgtc tgggcacgcc ggcgggcacc acccgcggct ttggcccggc
    22741 ggaattcacc cagatcggca agtggattgc ccaggtcgtc gatggcctgc gcaagaacgg
    22801 cgccgagggc gatgcccaga tcgaggcccg cgtggccgcc gaggtggaag cgctgtgcgc
    22861 cggcttcccg atctaccccg cgctgaggta ctgaggattg cgctgccctt tctgcgcctt
    22921 cgacaatacg caggttaagg attcgcggcc gacggaggac aataccgcca tccgtcggcg
    22981 gcggcagtgc gaaagctgcg gcgcccgctt cacgaccttc gagcgggtgc agctgcgcga
    23041 gatcaccgta atcaaggcct gcgacccgga atcgggcgag gcaaggcgcg aacctttcgt
    23101 gcgcgagaag atcgagcaat cggtggcgct ggcctgccgc aagcgcggcc ttgcgcagga
    23161 acggctggac cagctcgtct ccggcatcca gcgccagatc gagacgctgg gcgaaagcga
    23221 ggtgccctcg cgcgtgatcg gcgagatggt gatggatggg ctgcgcgggc tggacagcgt
    23281 ggcctatatc cgcttcgcct cggtctatcg cgattttgcc gcgccggaag atttcgagga
    23341 attcgccagc gcgatcaggg atgccggcgc cggaaaggaa cctgcatgag ccagcccccc
    23401 gtcatcgttc tcgtgcggcc gcagctgggc gagaatatcg gcaaggcggc ccgcgccatg
    23461 ctgaactttg gcttggccga catgcgcctg gtggcgccgc gcgatggctg gcccaacccc
    23521 tctgcgggcc ctgccgcggc gggcgccgac atcgtgctgg aaaaggcgca ggtgtttgcc
    23581 acgctggcag aggcggtggg cgattgcgcc catgtctatg ccaccacggt gaggaagcgc
    23641 ggcgtgacca agccagtgct gacgcccgag gaagccgcgc gcgagatcca cgcccatgat
    23701 ggcgccgacc ttggccgctc ggcgctggtg ttcgggccag agcgcgccgg gctggagaca
    23761 gaggatgtgg cgctggcccg ggcgatcgtc accgtgccgg tgaacccgga atttgcctcg
    23821 ctgaacctgg cgcaggcggt gatcctggcg gcctatgaat ggtccaaggg ggtgaaccgg
    23881 gccgcaggca cgctggcgca gcccacgcag gaagaactgc tgccccctgc cccccaggca
    23941 gagctggaag gcatgatcgc ccatgccgaa accctgctgg ccgagcgcga ctatttcttc
    24001 ccgccccacc gcgccgaggc cacccgcctg acgctgcgcg gcctgttcac caagccggcg
    24061 tggaaccatc tggaagtgcg caccatgcgc ggcgttctgg cagcgcttgt gcgccgcccg
    24121 ggcgaagtgc ccccgccccg gccccggccc gcgccgaaag ccgacgatca ggcttgactt
    24181 tcgcccgcaa ggccgccaaa gcgccgcctt cgcaatggac cgcgcacccc ggtgaagcgg
    24241 cggtcgcgcc cggcacaata ggccgggcgg tgcggaaccc gggatcacct gtggcagggc
    24301 aaccgccttg ccccatccga acgcaaattg gagttaccag cgcatgtcga agcgccacgc
    24361 atcgaagtac aagattgacc gccgcatggg cgagaacatc tggggccgcc cgaagagctc
    24421 ggtcaaccgc cgcagctatg gccccggcca gcacggccag cgccgcaagg gcaagatctc
    24481 ggacttcggc atccagctgc gcgccaagca gaagctcaag ggctactacg gcgatgtgac
    24541 cgagaagcag ttcaagcgca cctaccagga agcctcgaag atgaaaggcg acaccggtca
    24601 gaacctgatc ggcctgctgg aacagcgcct cgacatggtc gtctatcgcg ccaagttcgc
    24661 gccgaccatc ttctcggccc gccagatcgt cagccacggc cacatccgcg tgaacggcgt
    24721 gaagtgcaac atcgccagcc gccgcgtgcg ccccggcgac atcatcagcc tgggcaacaa
    24781 ggccaaggaa atggccctga tcgccgaggc gcaggccctg cccgagcgtg aagtgcccga
    24841 ctatgtggcg caggacagca ccgacaaggt gaccttcgtg cgcgtgccga cgctggacga
    24901 ggtgccctac cccgtgaaga tggaaccgaa cctggtcgtc gagttctact cgcgctaagg
    24961 ccggctccct cgggaacgca aaaaggcggg tggctttggc ctcccgcctt ttttgtgtgg
    25021 cccgggcaag tggccgcgcg cggcgcacgg cttgcccctt tgcgctggaa ccccaagcaa
    25081 attcagcccg ttatacaggc atgaaaatgc tctaccgata tgtgggggcc caaagggccg
    25141 gcaaatggta tcccgatctg gcgaccgccc agcgctttgc ctgcaagatt ggcgcaggct
    25201 ttctggcgga aaagagcggg cagttctgcg cttatctggg cacgcggctg gaagtgctgc
    25261 tgcccgatgg gcgcgtcatc cgccgacgga tgcgcgcccc ccagcccgcc gggcacttcc
    25321 ctccgggtca tttcccggcg aagtaatccc ggcggaaatc ggcagcaaag gcggcgaagc
    25381 gctgctcggc gatggcgccg cgcatggccg ccatcaactg ctgatagaag ctgatgttgt
    25441 gctcggtcat cagcatggcg ccgaggatct cgccggcctt gttgaggtgg tggagatagg
    25501 cgcggctgta ggtctggcag acatggcagc tgcagcgctc gtcgatcggg ccggtatcct
    25561 cggcatggcg ggcgttgcgg atgttgagcg ggccgttcca ggtgaaggcc tgcccgttgc
    25621 ggcccgagcg cgagggcagc acgcaatcga acatgtcgac gccgcgttcc accccgccca
    25681 ccagatcatc cggcttgccc acgcccatca gatagcgcgg cttgtcatcg ggcagcatgc
    25741 cgggggcaaa atccagcgtg gcgaacatcg cctcctgccc ctcgcccacg gccagcccgc
    25801 cgatggcata gccatcaaag ccgatctcgg tcagcttctc ggccgagatg cggcgcagat
    25861 cctcgtgcag cgcgccctgc tggatgccga acagggcggc gcgctcggca tgttcgccgc
    25921 cggcgttgaa ggcggcgcgg ctgcgcgcgg cccagcgcat gctcatttcc atggacgcgg
    25981 cgatgacatc gcgcgattga tccgcccgcg ggcattcatc gaagcacatg acgatgtcgc
    26041 tgcccagcag gcgctggatt tccatgctgc gttcggggct gagcatgtgg cggctgccat
    26101 cgatatggct ggcaaaagtg acgccttcct cggtgatctt gcgcagatcg gacaggctca
    26161 tcacctgata gccgccggaa tcggtgagga tcgggcgcgg ccagttcatg aacttgtgca
    26221 ggccgcccag ccgcgccacc cgctctgccc ccgggcgcag catcaggtga taggtgttgc
    26281 ccaggatgat gtcggcgccg gtgccacgca cggtttcggg cttcatcgcc ttcaccgtgg
    26341 ctgccgtgcc cacaggcatg aaggcaggcg tgcggatgtc acctcgcagc atctggatgg
    26401 cgccggtgcg cgccttgccg tcgcgcgcgt gaatctggaa agaaaatcgg gtcatgatgg
    26461 gttgcggccc atacgccttt tgggcaatcc acgcaaatgc ttgacccatg gatctatccc
    26521 gcgctgacgg cgacggcggt ttttacaggt ttcgtcgatt ccgtggccgg tggcggcggg
    26581 ctgatcatga tgcccgcgct gatctatgcg ggcgttccgc ccaccatgct tctgggcacc
    26641 aacaaggtgc aatcggcctg cggcacggcg atggccacct ggcgctatca ccgcgccggc
    26701 ctgtttgccc tgaagcccaa cctgccggcg gtggcggcga tctttgccgg cgccgccatt
    26761 ggcgcggcgg tgatccagag cttcaacgcc aatgtgctga agctggtggt gccggtgctg
    26821 ctgatgatgg tctcgctcta cacgctgttc tcgccgcgca tgcacgacga ggacagccac
    26881 ggccacatga gccagaaggg cttcctgccg gtgggcggcg cgctgggctt ctatgatggc
    26941 ttctttggcc ccggcgcggg gcaattctat gccaccgcgc tggtatcgct gcgcgggctg
    27001 gggctgaccc gggcaacggg cctcaccaag ctgttcaacg tcaccagcaa catcgccagc
    27061 atcctcgtct ttgccgcggg cgggcatgtg atctggctgc tgggcctgtg catgggcgcc
    27121 ggcgccatga ccggcaactg gatcggcgcc cacaccgcca cccgctttgg cgccaagctg
    27181 atccgcccgc tgctggtggt gtgcagcctg gggatgacgg caaggctgct gtggaactgg
    27241 tttgccggct gaggatcagc cccggccgcc cggcagcagc aggctggaat cgccatagga
    27301 atagaagcgg tagccctcgg cgatagcgtg ggcataggcg ccctgcatcc gctccagccc
    27361 catcagcgcc gacaccagca tgaacagggt ggagcgcggc aggtggaagt tggtcatcag
    27421 cccgtcgatc gcgcggaagc gatagccggg cgtgatgaag atcgaggtgt cgccggcaaa
    27481 ggggcggatc acgccatcct cgccggtggc gctttccagc aggcgcaggc tggtggtgcc
    27541 cacggcgatc acccgcccgc cggccgcccg cgccgcattc agccgctcgg ccacatcggc
    27601 gggcagcgtg ccccattcgg cgtgcatggc gtggtcttcg gtgttctcgg ccttcaccgg
    27661 caggaaggtg ccggcgccca catgcagtgt caggcattcg cgcgcgatgc cggcgccatc
    27721 cagcgcggca atcagttccg gcgtgaaatg cagcgccgcc gtgggcgcgg ccacagcgcc
    27781 atcgcgggcg gcgaacatcg tctggtaatc ggcgcggtcg gcctcgtcgg tggggcgctt
    27841 gccggcgata taggggggca gcggcatggt gccggcgcgc tccagcagca gttccaccgg
    27901 ttcctcgccg gcaaaggcca gggtccagct gccatctgca tggcgcgctt cggccagcgc
    27961 ttccacgccg gcgggaaagg cgatgacatc gccctcgtgc aggcgcttgc cgttcttcac
    28021 gaaggcctgc cagcgcctga gatcgatccg cttgtgcagc gtggcgccaa tcttcgcctc
    28081 gccgcgccgg ccttccagct gggcggggat aacgcgggtg tcgttgaaca ccagcacatc
    28141 gccggcgcgc agcagggcgg gcagatcgcg cacgcgcaga tcgtccagcc cgccgccccc
    28201 ggcgccatcc accagcagca ggcgggcggc atcgcgcggg cgggcgggcc tcagcgcgat
    28261 gcgttcggcc ggcaaatcga aatcgaacag atcaacctgc atcggtgaac agcggccgga
    28321 tcagttcgac ttggagtggt tgagctgatc gatgctgatc ggcgcgacgg ccttgatcgc
    28381 cgtcggcggg ggcggggcca gatgctggct ggccaccgag gcctggacga tccgggtggg
    28441 gttggccggc ggctcgcccc gctcgatgcc atcgaccaca tccatgccgg cgatcacccg
    28501 gccgaaattg gtgtagtgcc gatccagcgc catatgcgga tagaagacga tgaagaactg
    28561 gctgttggcc gaatcggcat catccgtgcg cgccatcgac acagagccgc gcacatgcgg
    28621 cacggtgttg aactccgcct tcaggttggg cagcttcgag ccgccgccgc cggtgccggt
    28681 gggatcgccc gtctgcgcca tgaagccatc gatcacgcgg tggaagatga tgccgttgta
    28741 aaagccctgg cgcgccagtt ccttgatgcg ttccacatgg tggggcgccc attccggcat
    28801 caggcggatt gccacgcgcc cgccgttcga cagatccagc agcaggacat tgtcgggatc
    28861 atgggcgata tcggtatcga cctgggccga gagcagcttg ccggtggggg ctggccccgc
    28921 cttcgccaga tgcagggtgg cggtcttgtg gccgcaggcg ctggtcgaca ggagcacgag
    28981 gcccatcgag aggcccagcg cgattctggc aagcggatgt cggttcatgc aactctcatg
    29041 cggtgggccc gggggcggtt gcccgggggc gaaagaaacg aaaggttccg gtgcacggcg
    29101 atagccgcgc gggccttctg tggcaatgcg gagcgtgggg ggcggagcgt agtggttggc
    29161 acaggggcac cggccgcacc gccggcgccg gccaggtcaa tccccctgca cgccgcctgt
    29221 ggccacgatc cgctcgatca catcggtgcg caccgcgggg ctgacgaatt tgccgatctc
    29281 gccgccgaac agggcgattt ccttgaccag cttggaggcg atcggctgca gcgagacatc
    29341 ggccatcagg aacaccgtct cgatgcgggc gttcagctgc tggttcatgc cggccatctg
    29401 atactcatat tcgaaatcgg ccaccgcccg caggcccctg acgatgacgc tggccccctg
    29461 cttctcggca aatttcatga gcagcgcgtt gaagcccacc accgagacat tctccagccc
    29521 catcgcggcc acttcacgct ccaccatggc gatgcgctcg accggcggga acagggggtt
    29581 cttggaaggg ttggtcgtca cgccgacgat cagccggtcg accagcttgg cgccgcgccg
    29641 gatgatatcg gcatggccca gcgtgatggg atcgaatgtg cccggataga cgccaaccct
    29701 ttcggccatt tagcgatccc tttccacgat gaagcgcgcc agttcgcgca gcaggtcggc
    29761 ctcggggcca tattgcgcca gatgggcgat ggactgatcc agcagcagcc gcgcctgttc
    29821 gcgcgcgcgc tcgggcccca gcagcgagac gaaggtttcc ttgcccttgg cctcgtcctt
    29881 gcgcagggcc ttgccggccg cttcctcatt gccctcgtaa tcgagcaggt cgtcggcgat
    29941 ctggaaagcc aggccaatat cgcgggcata gccgcgcaga tgggtgcgcc cctcgggcgg
    30001 cacatggccg aggatggcgc ccatttccac gcccgcgctc agcagcgcgc cggtcttcag
    30061 ctgctgcagg cgggtgatgg tggccagatc gaaggtgctc gtctcggcga cgatatccat
    30121 catctgcccg ccggccatgc catggtggcc gctggccagc gccagcgtct gcaccagttc
    30181 cacgcgggtg aaaggatcgc cgctggtggc aggatcagtc agcacctcga aggcgaaggt
    30241 caacaggctg tcgccggcca gcacggcggt ggcatcgtcg aaggccttgt gcacggtggg
    30301 cttgccgtgg cgcaggtcgt cattgtccat gcagggcagg tcgtcgtgga tcagcgaata
    30361 gacatgcacc gattccagcg ccacgcccac gcggatcgcc gcctcgcggt ccacgccgaa
    30421 cagttcggcc gtggccgcca ccagcagcgg gcgcacccgc ttgccgccgc caatcgcggc
    30481 atagcgcatg gcggcgatga ggcgcgcgcg cggatcatcg gtttcgggca gcagcgcgtc
    30541 gaaggcagca tcgatctcgc gctgaacgcg cttcaggctt tcggcgagca ggctgggagg
    30601 ttgggcggcc acaaaatatc cttaaagcaa gtgggagccg gcagcgcgat caggcgtcct
    30661 ggtcgaacgg gcgggtggct tggggggcgc cgcccggcgg ggcggccaca atcttctcga
    30721 tccgggcggc ggcggcatcg aggcgggcct ggcaggccag gcgcagcgcc tcgccgcgct
    30781 cgtagaggct gatcgactca tccagcggca cttcgccgct ttccagccgg cgcaccacat
    30841 cctccagcgc gcgcagcgcc tcctcgaaac tcagcgccgc gatccgcgcg gccatatcgg
    30901 cagcatcctg tgtcatcgcg cctgcaatgg ccggccgcgc ggccgcggtc aagcgcgagg
    30961 acgcggtggc gcgcgaggat caggccactc tagccttctg cccccaaggc ggctaaggcg
    31021 ctggcccatg agcacgcaag attccgccat cacgccagac gtcgtcgccg cccacgggct
    31081 ttccgaagag gaatatgcca aggttctcca tgcgctgggc cgcacgccca atctggtaga
    31141 actgggcatc ttttcggtga tgtggtcgga acattgctcg tataaatcca gccgcctgca
    31201 cctgaagaag ctgccgaccg aggcgccctg ggtgatctgc ggcccgggcg agaatgccgg
    31261 cgtgatcgac attggtgatg gccaggccgc gatcttcaag atggaatcgc acaaccaccc
    31321 gtcttacatc gagccctatc agggcgcggc gaccggcgtt ggcggcattc tgcgcgatgt
    31381 gttcaccatg ggcgcgcggc ccgtggccaa catgaacgcg ctgcgcttcg gccggcccga
    31441 tcaccccaag atgaagcatc tggtgcaggg cgtgattgcc ggcatcggcg gctatggcaa
    31501 ttgcgtgggc gtgcccaccg ttggcggcga gaccaacttc cacaaggcgt acgacggcaa
    31561 tatccttgtg aacgccatga cggttggcgt ggccgacacc gacaagatct tctattcggc
    31621 ggccacgggc ctgggcaatc ccatcgtcta tgtcggatcg aagaccgggc gtgacggcat
    31681 ccacggcgcc accatggcgt ctgccgattt cgacgagaag tcggaggaaa agcgccccac
    31741 cgtgcaggtg ggcgatccct tcaccgaaaa gctgctgatc gaggcctgcc tggaactgat
    31801 ggcgactgac gccatcgtcg ccattcagga tatgggcgcg gccgggctta cgtcttccag
    31861 cgtggaaatg gccagcaagg gcggcgcggg catccgcctc aacatgaaca atgtgccctg
    31921 ccgcgaaacc ggcatgacgc cctatgagat gatgctgagc gaaagccagg agcgcatgct
    31981 catggtgctc cagcccggca aggagccgat ggccgaggcg atcttcaaga agtgggagct
    32041 ggactttgcc gtgatcggcg aagtgaccga caccggccat atggtgctgg aattcgatgg
    32101 cgaagtggtt tgcgacattc cgctggcgcc gctggccgac gaagccccgc tctatgatcg
    32161 cccgcatgtg cccgccccca agcccgcgcc gctggccgat gcgcccgcaa gcgcggatat
    32221 tgccggcgat ctgctcaagc tgatcggcag cccggatctg gcctcgcgcc gctggatctt
    32281 cgagcagtat gacagccagg tgggcggcga cacgctgcag aagagcggcg gcgatgcggc
    32341 tgtggtgcgc gtgcatggca ccgacaaggc gctggccatc agcaccgatt gcacgccgcg
    32401 ctattgcttt gccgatccct atgagggcgg caagcaggcc gtggcggaaa ccttccgcaa
    32461 catctgcgcg gtgggcgcaa cgcccctggc catcaccaac tgcctgaact ttgccaaccc
    32521 ccagcgcccg gagatcatgg gccagatcgt cgggtgcctg gaaggcatgg gcgatgcctg
    32581 ccgcgcgctg gattacccca tcgtctcggg caatgtctcg ctctacaacg aatcgaaggc
    32641 gaccggtggc ggcagcgcca tcctgcccac ccctgccatc ggcggcgtgg gcctgatgct
    32701 ggaccacacg aaaatggcca ccatccgctt caaggccccg ggcgagacga tctggctgat
    32761 cgggcccgag ggcacgcatc tgggccaatc gctgtggctg cgcgagattg ccgggcgcga
    32821 ggaaggccct gcccctgccg tcgatctgga gctggagcgc cgcaatggcg agaccgtgcg
    32881 ccagctgatc cacgatggca ttgccacggc ggtgcatgat gtggccgatg gcgggttgct
    32941 ggtggcgatt gccgaaatgg cgatggccag cggccttggc gccgagctgg acggcagctt
    33001 caccgccgcc accgcctttg gcgaggacca gagccgctat gtgctgacga cccctgccga
    33061 tatcgtgctg gaaggggcga tccgcctggg ccgcgtgggc ggtgccagcg tggcgggcgt
    33121 ggaagtgacc cggctgaagg cggctaacga ggccttcttc aaggattgga tggaagcctg
    33181 atcaaggccc ggcgcgagag ggcccgcccc ctcgcgccgc ctttggttca gcccttcacc
    33241 cactcgcttt cgcccacgcc cagcaggcgc agcacggcgg tgaggtcccc ccggtcgatc
    33301 cagccattgg ccgcggcgcg cgccttgggc ttggcggcaa aggcaatgcc ataggtcgcc
    33361 gcttccatca tcgggatatc attggcgcca tcgccggtgg ccagcgtatg agcgccctcg
    33421 cccagcgcgg ccagttcctc cagcagcgtg gccttcttgg tggccgaatc gctgatcggg
    33481 ccaaccaggc cgccggtcag cacgccgcct tccacgccca gccggttgcc caccacccgt
    33541 tcaaagccca gcgcctcggc caccggatcg gcgaactggt ggaagccgcc ggtcaccagc
    33601 acggtgcggc tgcccttggc cttcagcgtc tgcaccagcg tgcgggcgcc ctgcatcggg
    33661 tggatgcgct ctgccaggca gcgggcaatg gccccttccg acaggccctt cagcagcgcc
    33721 acacgctctg ccagcgccgc gcggaaatcc agctcgccct gcatggcgcg ctcggtgatg
    33781 gcggcgatct ggggcttgag gttggcaaaa tcagccagtt cgtcaatgca ttcggccgag
    33841 atcatggtgg aatccatgtc cgagacgaac agcgccggca gctgcggcac gcccgcgccg
    33901 atcagcgcat ccgtctgccc cagatgcgcc tcaatggcag agcgcagggc atcggcgccg
    33961 gcatcgctct ccagctccag cagggctgcc gggccatttt ccagcacgcg cgccgcgctg
    34021 tcgcggccct ggctgcgcaa tgtggcgcgc aacgcttcca cccttgccga aagcccatcc
    34081 gaatctgcta tcacgcgcgc aatgaccacc aacaccatcc ctgaaaccga tcgcggcccg
    34141 gggcggccgc cgctggcgct cattgcaggg cccacggcca gcggcaagag cgattgtgcg
    34201 ctggcgctgg cccgctggct ggccgcgcgc gggcgccagg cggtgatcgt caatgccgac
    34261 agcgcccagc tctatgccga tctgccggtg ctttcggccg cgccctcgcc ggcggaacag
    34321 gccgaagtgc cgcaccggct ctatggcgcg tgggatgggg ccgagggctg ctctgccgcc
    34381 gattgggccg cccgcgcgcg cgatgagatc gacgtggcgc acaaggccgg catggtgccc
    34441 attctggtgg gcggcaccgg gctttacatc cgcacgctgc tcgatggcat tgcccctgtg
    34501 ccggcgatag acccggccgt gcgcgcggcc gtgcgcgccc tgcccgtggc ccaggcccat
    34561 gccgcgctgg ccagggaaga ccccgccgcc gcccggcgcc tgcacccggc cgacaccacc
    34621 cgcgtggccc gcgcgctgga agtggtgcgc gccaccggcc tgccgctggc cagctggcag
    34681 gcgcgcaagg aaggcggcat cggccacagg gtggcgctgg cgccggtggt gctgctgccc
    34741 gaacgcggct ggctctatgc ccgctgcgac cggcgtttcg aacgcatggt ggaggcaggc
    34801 gccgtggcgg aagtggccgc cctgctggcg cgcgggctgg cggccgatct gccggtgatg
    34861 cgcgccatcg gcgtggccga gattgccgcc caccttgccg gcgattcgga tctggcaacg
    34921 gcgatcgccc gcggccagca ggcgacacga aattacgcca agcggcaata tacatggctt
    34981 cggcaccagc cgccgcaaga ttggccgcgt caaacttcat ctgacatcga tttttctccg
    35041 tattttgaaa tattattacc ttaaatcccg ttgacggtga acttttagat ccatagagac
    35101 aggccatcac ctgccgaggc aggtcttgcg cgagttggag aggaggcccg gtgcggttca
    35161 caccgtatcg ggccatcctt ttcccggccg ggatggccca gtgcccttcc cttcgccagg
    35221 cgtcaggccc tgtgtcggca cacccgaggc aacaaaggat attccccgtg agcgacgagc
    35281 gtagcggcgc ccaaattctt gtcgaaagcc tggtccgcca gggggtcgaa catgtgttcg
    35341 gctatcccgg cggcgcggtg ctgccgatct atgacgccct gtttggcgat gagcgcctgc
    35401 gccatatcct cgtgcgccac gaggcggccg cggcccatgc ggccgaaggc tatgcccgct
    35461 ccaccgggcg gccgggcgtg gtgctggtca cctcggggcc cggggcaacc aacgctgtca
    35521 ccggcattgc cgatgccttc ctcgattcga tcccgatggt cgtcatcacc ggccaggtgc
    35581 caaccgcgct gatcggctcc gacgccttcc aggaagccga cacgatcggc atcacccgcc
    35641 actgcaccaa gcacaactat ctggtgaagg acccggcaga ccttgccgcc actatcgacg
    35701 aggccttcca ggtggccacc agcggccggc cgggccctgt cgtcatcgac atccccaagg
    35761 atgtgcagat cgccagcgcg gtgctttccg atgcgccggt gcagcgccgc cagcgctatg
    35821 ccccgcagac ccgcggcgcc gacaaggaca ttgccgccgc gatcgagctg ctggcccatg
    35881 ccaaggcacc ggtgttctac accggcggcg gcgtcatcaa ttccggcccg cgcgccaccg
    35941 aactgctgcg ccagctgcag gcgatcatcg gcgcgccggt cacttccacg ctgatgggcc
    36001 tgggcgcctt ccccgccacc catgccgact ggctgggcat gctgggcatg catggcacct
    36061 atgaggcgaa ctgggcgatg aaccaggccg atctcatcct gtgcgtgggc gcccgcttcg
    36121 acgaccgggt gaccgggcgg ctggatgcct ttgcccccaa ttccaccaag atccacatcg
    36181 acatcgaccg cgccagcatc aacaagacgg tgccggtgga cctgccgatc atcggcgatt
    36241 gcgccgaggt gctggagcag ctgatcaccg cctggggcaa ccgcaagccg caggaccttg
    36301 ccgagtggaa ggcgcgcgtt gatggctggc gggccaagaa gtgcctcgcc tatcccgcct
    36361 atggcagcga gattgccccc cagcgcgcga tcgagcggct gcatgcgctg acccatgcgg
    36421 tggacccgat catcagcacc gaggttggcc agcaccagat gtgggcggcg cagtatttcg
    36481 gcatggaaga gcccaaccgc tggctcacct cgggcgggct gggcacgatg ggctatggcc
    36541 tgccggcggc gatcggcgcg caggttggca atcccggccg gctggtcatc gacattgccg
    36601 gcgaggcctc cattcagatg aacatccagg aactgggcac ggccagccag tatcgcctgc
    36661 cggtcaaggt cttcatcctc aacaatgaat ggatgggcat ggtccgccag tggcaggagc
    36721 tgacctatga gagccgctat tcgaactcct attcggacag cctgcccgat ttcgtgcggc
    36781 tggccgaggc ctatggctgg aagggcattg ccattaccag cgaggcggag ctggatgccg
    36841 gcatccaggc gatgatcgac catgacgggc cggtgatcgt tgattgccgg gtggccaagg
    36901 ccgccaattg cttcccgatg atcccttcgg gcgccgccca caccgaaatg ctgctgcccg
    36961 gcgacgtggt ggccggcacg atggacgatg aagccaaggc gctggtctga ggaacctacg
    37021 agatgatgaa gatcaagcat gccgccgagg agcgccatgt cctcactgtc ctggtcgaca
    37081 atgaggcggg cattctcgcc aagatcgccg ggctgttcac cgcccgcggc tataatatcg
    37141 acagcctgac cgtggccgac atcaccgaca accacgatgt cagccggatc accatcgtca
    37201 cgcacgggcc gccggcggtg atcgaccaga tccgcgccca gctcgaccgg ctggtgccgg
    37261 tgcacaaggt gatcgacctc accgaggtgg gcccccatgt ggagcgcgag ctggcgctca
    37321 tcaaggtggc cggcaagggc gagaaccggg tggaggcgct gcgcatcgcc gatctgttcc
    37381 gcgccaaggt ggtcgatacc tcgaccagca gcttcatttt cgagctgacg ggaacgcccg
    37441 acaagatcga cagtttcatt gccctgatgc gcgagctggg gctggtcgaa gtggggcgca
    37501 ccggcattgt cggcatgacc cgcggcggcc aacccgcctg aggcgcgaat agcgccccgg
    37561 ccgccatcca aacttgatca tggccccctg atagtgcagg gggcgccacc aacggggctt
    37621 ttgccctgcc ccatggcagg caaggcccaa ccatcctggc tccccttggc cctcgggcgg
    37681 gagcagcaaa ggggaacgac cgtgaaagtc tattacgacg ccgatgccga cctgaacctg
    37741 atcaccggca agaagatcgc catcctgggc tatggctcgc agggccatgc ccatgcgcag
    37801 aacctgcgcg acagcggcgt gaaggacgtg gcgatcgccc tgcgcgaggg ttcggccacg
    37861 gcgaagaagg ccgaagctgc cggcttcaag gtgctgagca acgccgaagc cgccgcctgg
    37921 gccgacgtgc tgatgatcct ggcgcccgac gagcaccagg ccgccatcta tgccgcggac
    37981 ctgcatgcca acatgaagcc cggcgcggcg ctggcctttg cccatggcct caacatccac
    38041 ttcggcctga tcgaggcgcg cgccgacatc gacgtgatca tgatcgcccc caagggcccg
    38101 ggccacaccg tgcgcggcga atatcagcgc ggcggcggcg tgccctgcct gatcgccatc
    38161 caccaggatg tgaccggcaa cgcccatgac atcgcgctgg cctatgcctc gggcgtgggt
    38221 ggcggccgtt cgggcatcat cgagaccaac ttccgcgagg aatgcgaaac cgacctgttc
    38281 ggcgagcagg ccgtgctgtg cggcggcacc accgcgctgg tgcaggccgg cttcgaaacg
    38341 ctggtggaag ccggctatgc ccccgagatg gcctatttcg agtgcctgca cgagctgaag
    38401 ctgatcgttg acctgatgta tgagggcggc atcgccaaca tgcgctactc gatctcgaac
    38461 accgccgaat atggcgacat cgtgaccggc ccgcgcatca tcaccgaaga gaccaagaag
    38521 gaaatgaagc gcgttctggc cgacatccag tcgggccgct tcgtgaagaa cttcgtgctc
    38581 gacaaccgcg ccggccagcc cgagctgaag gccagccgca tcgccgccaa gcgccacccg
    38641 atcgaggaaa ccggcgccaa gctgcgcgcg atgatgccct ggatcggcgc caacgccctg
    38701 gtcgacaagt ccaagaattg atccccggcg caaggcatct tgcgcccggg aacaaccaag
    38761 ccgccggtgg gccccgcgcc cgccggcggt tttgcatgaa ggccgatccg ctcttgcctt
    38821 ggccgccgaa cgcgcccaca gcagcgccca tgccgcatcg cgccgaccac attctcatct
    38881 gtttccacga tttctcgcgc ggcggcacag aacgcatcgc cattggcctg gcccgccact
    38941 gggccgagca gggccggcgc gtcaccatcc tgtgcggcac cacagagggc ggcctcaggg
    39001 ccacggtgga tgcccgcgtg caggtggtgg agctggatcc gcccgtgcgc cgcagcctca
    39061 cctcgcggct gaagctgggc aaggcgatgg ccgccgaaat cgcccgcctc aagccagaca
    39121 tcatcttcct gcccggcaat ttccacctgc cgctggcccc cgccttcgcc cgcattcccg
    39181 gccgcgtcga ggaaggctgg ggggcgatca tggcgaagat ctccaacccc tcggtgcccg
    39241 atgggctggc cgcggccccg gtgcgctggc tgctgcgccg ctttggcccc tgcgtggatg
    39301 gctgggcggc gatgaattcc gggctggagc gcgaattgca cgcgatcctg ccgggcgccc
    39361 atgtggaaac gctgttcgat ccggtctacc tccaccacga tctggccccc gacgaccatc
    39421 ttgccccaac cgacgagcgg ctggagatcc tgtgggccgg ccgcttcgag ccgcagaagg
    39481 acacgctgct ggcgctgaag gtgatcgagg cgctgaacga gatcgtgccc gcccgcctca
    39541 ccatggtggg cgacgggcgg ctctatccgg ccaccttgcg cgccatcaag cgcatggggc
    39601 tgacagatat catcgccgcg cccggctatg tgccgggcat cgatccctgg ctgcggcgcg
    39661 ccgatgcgct gctcatcacc tcgcatttcg agggcgggcc ggcggtggcg gtggaagcgc
    39721 tggcccatgg cgtgccagtg gtttccaccg attgctcgca tttcctgcgc gatatcatgc
    39781 gcgtgccgct ggccgggcgg atcgtgccct cgcgctatgc gcccgatctg gcctcggcgc
    39841 tgctcagcac ggtgtcggaa gggcggcgcg atccggcagc gctccatgcg ctgatcgatc
    39901 atctcgatcc gcgcgtgtgc gcgcaaggct atctggcctg gtttgatcgc atggcggtgg
    39961 cccgccgctg agccgcatgg cggcggcccg ccgctgagcc gcatggcagc ggcccaaggc
    40021 gaagaaagcc gctttacccc ctttacacag cgcccatcca tccccatagc caatgccatg
    40081 cacacgcgcc ttggccttac cctgcgactt atccgccctt gggcgtgaac gggcgtgcct
    40141 gccgttgggg cgcgccacgc gtccaagggt gaaacttcgc aaccccggcc acaatcgcag
    40201 gttttcgaga taacgccatg agcatgctga acgacccttc cgtcaaatat cgccccttcc
    40261 cgcaggtcaa cattccggat cggcaatggc cttcgcgcac catcaccaag gcgccgcgct
    40321 ggctgtcgac ggacctgcgc gatggcaacc agagcctgat cgacccgatg ggcgccgaga
    40381 agaagacccg cttcttcgac ctgctggtga aggtggggct gaaggagatc gaggtcggct
    40441 tcccgtcggc cggcgccacc gaattcgact tcatccgcgg cctggtcgat aatggccggg
    40501 tgcccgatga tgtgctggtc caggtgctga cgcaaagccg cgaagacctc atccgcacaa
    40561 gctttgaatc gctggccggc gcgcctgcgg ccatcgtcca tgtgtataac gccgtctcgc
    40621 cgctgtggcg ccaggtggtg tttggcatgg aaaagcccga gatcaaggaa atcgcccgca
    40681 acggcgccag gctgctgcgc gacaatgccg cccgcttccc gcagacgaag tggcatttcg
    40741 aatatagccc ggagaccttc tccaccgccg agctcgattt ctcgatcgaa tgctgcgagg
    40801 cggtgatgga gatcctgcag cccacggccg agcatccgat catcctcaac ctgccggcca
    40861 cggtggaagc ttccaccccc aacatctatg ccgaccagat cgagtatttc tgccgcaacc
    40921 tgccggggcg cgaccgggcg atcatcagcc tgcacaccca taacgaccgc ggcaccggcg
    40981 ttgccgcggc cgagctgggc ctgatggccg gcgccgaccg cgtggaaggc tgcctgttcg
    41041 gcaatggcga gcgcaccggc aattgctgcc tggtgaccgt ggcgctgaac ctctacacgc
    41101 aaggggtgaa ccccgagctg gacttctcgg atatcgacga ggtgatccag acggtggaat
    41161 attgcaacca gctgcccgtt caccagcgcc acccctatgg cggcgagctg gtgttcaccg
    41221 ccttctccgg cagccatcag gatgccatca agaagggctt tgccgcgcag gaacagagga
    41281 acgaccagct ctgggcggtg ccctatctgc cgatcgaccc ggcggatctg ggccgcagct
    41341 atgaagcggt gatccgcgtc aattcgcaaa gcggcaaggg cggtttcgcc tgggtgctgg
    41401 agcaggacca ggggctgaag ctgcccaagc gcatgcaggc ccatttcagc cgccatgtgc
    41461 agcagctggc cgacgagctg ggccgcgagc tgcaggccgc cgacatctgg gacgtgttcc
    41521 agcgcaccta tcgggtgaac gacccgcagc acctgcagct gctggactgg gacgaggcca
    41581 ggggcgccga tggcagccgc atctttgccg gcaagatcgg cgtggatggg caggaacagt
    41641 cggtctcggg ccggggcaat ggcctcatct cctcggtggt ggcaacgctg gccgacagtt
    41701 tcggcgtggc gctgagcgtg aaggattatt ccgaacacgc catgggcgcc ggcaccgatg
    41761 cccgcgcggc cgcctatgtc gaatgcaccg gccccgatgg gcagacggtg tggggcgtgg
    41821 ggctggatac cgatgtggcc accgccagcg tgcgggcggt gctctcggcg gccaattcgc
    41881 tgatcgggcg ctaacgaacc actagctggc cccgaccacc accagcgtgg ccggggccag
    41941 ttcctgcgtg ctgccccaca ggctgtcggc cagcgcatcc agctcggcga aatcggcatt
    42001 atacagcacc ggctgcgccc ggttcagccg caagggccgc cgcccgggat cgagcagccg
    42061 ccggcgcagc gccctttgca gcagccgcag ccgcgccagc gcctcgctca gcgccacttc
    42121 gggcgcgcgc agccgccggc acgaccaatc cgcctccacc tgccccacgc gctctgtcac
    42181 catggcgtta tagtcgcggt gggggccgcg gtgcagcggc aggccaatgc gcgccgcccc
    42241 cgcatcggtg gccggcagca gcaggccatt ggcgcgaaaa tcatcaaagc ccagccgctc
    42301 gcgcccgatc gcctcgaaca gcgtgccaaa gcagcggccc tgcagcagct ggcgcggcaa
    42361 caggtggtgg cgctgcaggc cgggatcgta atcggccgca tcgcgccggt tcaccgcgcg
    42421 gaatggcagc cgccggcgcg ccgtttcctg ccccgcctcg tgcccggccg cctcctgccc
    42481 ggccgacagc ggcagaaaca gcccgcatgc cggcagcgcg gccggcagca catggtggcg
    42541 cggcgcgccc ggccccatgg cgctcacgat ggaagcgccg ccgagccctg tgtcattctg
    42601 gcatgaaaaa agaaattccg cagacttatc cagctgcacg ccatacacgc cacaggcctc
    42661 cactgcgagc aggctctatc cgcaagacct cgccttccgg cccatccacc aggcaggggg
    42721 gcgagatatc gccgccgctg acgagactgt ggataagccg aatcgcgatc ttgccatcgc
    42781 cccctgcccc attttgggac gttggttcga ttcgcaattc ctgtccagtg aaaaaggcca
    42841 gcccatcgct gcagatggcg ccatcggcct cgcgcaggag ggcggtgagg cccagcgccg
    42901 gaaacgcccc cccctccagc cagcggccca cattgcgcag gaaataatcg cgccccatca
    42961 tcgtcgccgc cggcgcccag atcaccgctt ccaccccgcc cagcccggcc agatcgaccg
    43021 ccagctgcat catcgcgcgc accaccggca gcaggttttc gccgcccgcc agatgggcgc
    43081 cgggccgcag caccaccgct tccagcggcg cctccggatc ggggccggcc acgccaaagc
    43141 ggtggcgcac ggcaggggtt ggcagggcgg cagccggcgc cagccccacc agatcgaagg
    43201 caaggccggt gatcagcagt tccagccagc cctcttcggc gggcgggcgg tggctgatag
    43261 caaagtggcg ggcagcgccg ggctggctgg ccaacagctg cgccaccgcc tcggcatccg
    43321 cgcgccggcc ggccgaaaac aacaggagga ccgccgaggc ccctgcccgg ggcggcgcat
    43381 attctgccgt catgtgacta atataaccgc ccctaggcca aactgcgcgg gggtatgcca
    43441 tcgcgccggc gctgcgtcga gtctcaccgc gccttgggcc cgccgcttgc gctcagaaga
    43501 tgcccagcgc cagccccgcg cccagcgccg cgcccagatc gcgggcctgc gcgcgctgat
    43561 cgggcgagag gcgcttgggc gcgcaaatct gctcgggcgt ctgcgcgccc atgttgatga
    43621 tcagcggcgg cgccacccgc ttcagccgcc agccggtggc gatgcgatcg atctgcgcct
    43681 cggcgccatg gccatcgctg ccggcagcga tgatcgttgc ataggggcgc ccctcggcgc
    43741 ggcccagcag cgggtaatag gcgcggtcga acatctcctt catcgccccg ctcatgctgg
    43801 ccaggttttc cgggcagacg aagagatagg ccgccgctgc cagcagcagc tccggccgag
    43861 cccgggcggc ggccaccagc cgcacctccg cgccctgccc cctggcgccg gccagtgccg
    43921 cgcgggccag cgccgcgctc gcacctgtcc ggctgtgcca cacgatcagt aacatggcct
    43981 gtcatccaca tccgcgccgc aaccaccgct atgcgccggc gcccccttgc gataggccac
    44041 agccatgagc atccggcaag ccccctctcc tgcagaaccc gcccgcatca cccatatggg
    44101 ccaatcgctg accgcgcgcg cctggcgctg gcgcggcggc aacatggata tcgccgggga
    44161 tggcgcgctg gcagcccaca cgccgggcga ggatctggtg acgcagctgc tgctggcccg
    44221 cggcgtggcg ccggccgatc tggagcggca gcgcaccccc accctgcgcg ccttcctgcc
    44281 cgatccttcc gtgctgcagg atatggacaa ggccgccgcg cgcctggccg atgccgtggt
    44341 ggccggcgag gcggtggccg tctatggcga ttatgacgtg gatggcgcca ccagcgcggc
    44401 gctgctgctg cgcttcttgc gcatgctggg gcgcgagggg cggccctata tccccgatcg
    44461 gctgctggaa ggctatggcc ccaccggtga ggcgctgctg cggctagcgg ccgaaggcgc
    44521 gcagctgatc ctcactgtcg attgcggggc gatggcgcat gaggcgctgg cgatggcggc
    44581 cgatggcggc gtcgacgtga tcgtggtgga tcatcacaaa tgcgcggcag agctgccccg
    44641 cgcgctggcc ctcgtcaacc ccaaccggct ggatgaaagc ccggaagggg caagcttcgg
    44701 ccatctggcg gcggtcggcg tggccttcct gctggccatc gccaccgcgc gcgagctgcg
    44761 ccgccggggc tggtttgccg gaaggcaaga gccggacctg ctcgccctgc tcgatcttgt
    44821 ggcgctgggc acggtggccg atgtggcagc gctgcatggg ctgaaccggg cactggtggc
    44881 gcaggggctg aaagtgatgg cccggcgcga gaatatcggc atggcagcgc tggccgattc
    44941 cagccgcctg tcgcgcgcgc ccacctgttc ggatctgggc tttgcgatgg ggccgcgggt
    45001 gaacgccggc ggccgagtgg gcgaggcgat gctgggcgtg cgcctgctga cctcggattc
    45061 gcccgaggaa gcgcgcgaga tcgccgccca gctttcgcgc cacaatgagg aacgccgcgc
    45121 catcgaggcc gccgtgcagc aggccgccga agccctgatc gatgcccagc acaaccgttc
    45181 ggtgctggtg ctggcgggtg aaggctggca cccgggggtg atcggcattg tggccgggcg
    45241 gatcaaggag aagaccggca agcccacttt cgtggtcgcg ctggatgggg cccagggcaa
    45301 gggctcgggc cggtcggtgg cgggggtgga tctgggcgcg gccgtcatcg ccgcgcgcga
    45361 ggcggggctg ctggtggccg gtggcggcca tgcg
//
LOCUS       DASRYE010000120        11803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_22501,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000120 DASRYE010000000
VERSION     DASRYE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22501"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2515)
                     /locus_tag="VFF98_06790"
     CDS             complement(<1..2515)
                     /locus_tag="VFF98_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZU63370.1"
                     /translation="MIKAGLYHHTRYSYSKPVRLGPQVIRLRPAAHSRTAIPNYSLKI
                     SPENHFLNWQQDPHGNWQARVVFPDPVDHFSIEVDLLADLAVVNPFDFFVEPQVEELP
                     FAYSEEQQQDLAAYFETEPQGPLFDALAAEYAAIIASGKAGRTIDFLVMVNQALQQRI
                     GYVIRLEQGVQSPEETLNLASGSCRDSAWTMVQLLRQLGFAARFVSGYSIQLVADVLA
                     IDGPQGVLEDICDLHAWCEVYIPGAGWVALDTTSGMFAGEGHIPLCAAPHYRSATPIE
                     GGFTTLDGDHVDSDFHFEMHVSRVREAVRITKPFTDERWEALLALGEKVDADIAAQDM
                     RLTMGGEPTFIADGDFDAPEWKSEAVGPTKAAYADRLIRKLHKAFAPGAVLHHGQGKW
                     YPGETLPRWGYSLYWRRDGVPIWHDPTLVAGEYPEKGLLPQDRPEDKPTAPVTTETAR
                     AVLEEAGIRLGLAPGFVQPVYEDTIQWIVQEAELPENTSPLDPALEDPEQRARFMRTF
                     QRGLTKPVGHVLPVQRWQKANEAPRWMSEVWRVRRGLLYAVPGDSALGYRLPLGALPY
                     VAAGDYPYIHPRDTAEPRAPLADFRRQLAERGFETREALALRGPQQPPRQPRAEQTIT
                     EIEGVVRTAITAEARPGPDGGPEGGYVAVFVPPVSTLEDWLELIAAVESVAEAIRVPV
                     RIEGYPPPPDPRLAVLKVTPDPGVIEVNIHPAASWAEQVEITETLYQAAREVGLTADR
                     FMVDGRPMGTGGGNHIVLGGPSLLDSPFVRRPDLLKSFVIYWQRHPALSYLFSGLFIG
                     PTSQAPRVDEARHDALYELEIALSQVPGPHQPQPLAWVVD"
     gene            complement(2570..3628)
                     /locus_tag="VFF98_06795"
     CDS             complement(2570..3628)
                     /locus_tag="VFF98_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZU63371.1"
                     /translation="MPPSPAIAPAPQLLLPRRHVLAGLGAGALGSLGGPLLAAGRPIA
                     PRQRVIIDNDFAGDPDGLFQLAHHLASPSVEIPLIVGSHLHKGEFFDPSARQADHAAQ
                     KAATIAGLMRLARVPRIIAGANAAMAADAPTPASAAIIAEALRDDTQLPLFYAAGASL
                     TELATAWLAEPRIGRRLVLVWIGGPEHADLLPGLPQRHDAEYNLTIDTAAAEVIFNRS
                     DIEIWQVPRNVYRQMLVSRAELAAALAPARALGRYLLAAIEHVAALTEAAPYHVNIGE
                     TYILGDSPLVTLTALQSSFEPDSASSLYTWRPTPRIGADGAWQPAPTNARPMRVYEHI
                     DTRLTFADMFAKLAAAAR"
     gene            complement(3732..3818)
                     /locus_tag="VFF98_06800"
     tRNA            complement(3732..3818)
                     /locus_tag="VFF98_06800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3782..3784),aa:Leu,seq:cag)
     gene            4079..5032
                     /locus_tag="VFF98_06805"
     CDS             4079..5032
                     /locus_tag="VFF98_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HZU63372.1"
                     /translation="MALPLSETSLQDKLVVLLGGGGFVGTHVAQELLARGARLRIASR
                     HPERAYALKTLGNLGQVQFARCDLARPDSVKRVLAGADAVVNLVGAFAGKLDAIQGSG
                     LGAIAGAALEAGASAFVQVSSLNADAGSQVAYARTKAEGEAAVLAAFPEATILRPSII
                     FGQYDGFVNRFAQLVAGLPVVPVFVPGAQLQPVFVDDVAEAVAITLADPETHGGKTYE
                     LAGPETVSMQAVMERIATACGRDPLLLPVCNLGAGLFAALPGTPLSTDQLHLLKAGSL
                     ATGSLPGLAELGITARPMELFLDRWLMRYREHGRFGVKRAA"
     gene            complement(5100..5771)
                     /locus_tag="VFF98_06810"
     CDS             complement(5100..5771)
                     /locus_tag="VFF98_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU63373.1"
                     /translation="MWQLYQFPLCPFSRKLRLLLSEKGIAYELWRENPWERRDAFLAM
                     NPAGHTPVLRHAAKGAVLADSRAICEFIEETESGAPMISGTAYQRAEIRRLVALFDES
                     LHADVVGPLLYERMKKRLVLRQSPDSRVLRDAMKLAHEHLYYIDYLLDHRPWLAGPTM
                     SLADLAAAAQISVADYLGGIDWQGHEQTKAWYSVLKSRPSFRPLLAERMEVIQPPAHY
                     ADVNA"
     gene            complement(5846..6667)
                     /gene="fdhD"
                     /locus_tag="VFF98_06815"
     CDS             complement(5846..6667)
                     /gene="fdhD"
                     /locus_tag="VFF98_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZU63374.1"
                     /translation="MTAIAPPEPLPPLPQADWPALAADGGRGAARRALVPEAPVALEC
                     NGLAYAVMMATPVDLADFATGFALAEGLAPTARAIADIAVAEVELGWIIRATIPTLGA
                     EQLGERVRRRVAESSCGLCGVENLAALAKPLPPVPPHARLESGAIFAALAALGPVQAL
                     GRATGAAHAAAFCAADGTLVAWREDVGRHNALDKLVGAMARAGADMAAGFVLSTARCS
                     YEIVEKAVRAGAHTLVTISLPTSLAVARAQAAGLSLWVLARGDSVLLAHDGAQPG"
     gene            complement(6667..7041)
                     /locus_tag="VFF98_06820"
     CDS             complement(6667..7041)
                     /locus_tag="VFF98_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU63375.1"
                     /translation="MFSHVFVGSNDLARAKIFYDALFAALDGSTGLIDEQIGRIVYRH
                     AGALFIVCRPINGEPATVANGGTIGFGMASEEQVNAWHAAGVAHGGTAIEDAPGQRPN
                     GLYLAYLRDPDGNKLCALHRPA"
     gene            complement(7215..8651)
                     /locus_tag="VFF98_06825"
     CDS             complement(7215..8651)
                     /locus_tag="VFF98_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZU63376.1"
                     /translation="MGKETGFLELDRQDRTYADPKERIHHYKEFVIPHAEGALKAQAS
                     RCMNCGIPYCHNGCPVNNIIPDWNHLTYEGNWRGALEVLHSTNNFPEFTGRICPAPCE
                     AACTLNIIDQAVTIKSIECAIVDKGWEEGWITPQVPAHKTGKAVAVVGSGPAGLACAQ
                     QLARAGHSVTVFEKSDRVGGLLRYGIPDFKMEKHLINRRMVQMEAEGVSFRTSTEVGV
                     SVSVASLKDNFDAVVLTGGAEEPRPLGIPGFELAGVRFAMEFLTQQNKRNAGDDEIRA
                     APRGSLTATGKHVIVIGGGDTGSDCVGTSNRQGALSVTQLEIMPEPPVKEDKALTWPN
                     WPLKLRTSTSHEEGVTRDFAVLTKRVIGENGVVTGLECTHVEWAGGKMTEVPGSTFTL
                     KADLILLAMGFVGPKKAGLIDQSGVALDGRGNVQANTEDYRTSDPKIFAAGDMRRGQS
                     LVVWAIREGRQAARAVDEALMGASELPR"
     gene            complement(8680..>11803)
                     /gene="gltB"
                     /locus_tag="VFF98_06830"
     CDS             complement(8680..>11803)
                     /gene="gltB"
                     /locus_tag="VFF98_06830"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZU63377.1"
                     /translation="TDTPIAVLSRKSRLLFDYFKQNFAQVTNPPIDPIREELVMSLVS
                     MIGPRPNLLGHDAGSHKRLEIDQPILTNADVAKIRSVEAALDGAFRTATIDTTWDAAS
                     GAEGLEQAIKEMVWAATEAVLADKNILILSDRAQGPDRIPMPAALATAAVHHHLVRQG
                     LRMQTGLVVETGEAREVHHFCVLAGYGAEAVNPYVAFETLEDIRERKELPLSAYEVQK
                     NYIKAIGKGILKVMSKMGISTYQSYCGAQIFDAVGLSSAFVEKYFSGTATTIEGAGLK
                     EVAEETVRRHAAAYGDNPIYKHMLDVGGIYGYRLRGEEHAWTPGNIASLQHAVRGNIP
                     EKYKEFAETINDQATRNLTIRGLIELKKGDRPLDISEVEPASEIVKRFATGAMSYGSI
                     SWEAHTTLAIAMNRIGGKSNTGEGGEDPKRFVALPNGDSLRSSIKQVASGRFGVTAEY
                     LVNADDIQIKMAQGAKPGEGGQLPGDKVDKTIGKTRHSTPGVGLISPPPHHDIYSIED
                     LAQLIHDLKNVNTGARISVKLVSEVGVGTVAAGVSKARADHVTISGYEGGTGASPLTS
                     LTHAGSPWEIGLAETQQTLLLNNLRSRICVQADGGLRTGRDVAVAALLGADEFGFATA
                     PLIAAGCIMMRKCHLNTCPVGVATQDPVLRARFTGQPEHVINYFFFVAEELRAIMAEM
                     GFRTIAEMVGRVDRLEPKKAVNHWKAQGIDLTKLLYQVPGDASPSLNWSQTQDHGLDG
                     ALDNELIAAAADALVESKPVRIERRVINVNRTVGAMLSGEVAKRYGHAGLPDNTIHIS
                     LTGTAGQSFGAFLAHGVTLELTGDGNDYVGKGLSGGRVIVRQPAFVQREPTENIIVGN
                     TVLYGAISGEAFFAGVAGERFAVRNSGAIAVVEGTGDHGCEYMTGGVVVVLGKTGRNF
                     AAGMSGGVAYVYDEAGNFDKLANQAQVDLLPVSAERDEEDGAGRPQQRAISVQDLGMG
                     DMLRHDAERLRILLERHHLYSGSARARALLDDWANTLGKFVKVMPRDYAKALQAMDAE
                     NQAAATEAAE"
BASE COUNT         1859 a         4008 c         3975 g         1961 t
ORIGIN      
        1 ggtccaccac ccaggccagc ggctgcggct gatgggggcc gggcacttgc gaaagggcga
       61 tctccagctc gtaaagcgca tcgtggcgcg cttcgtccac gcgcggggcc tggctggtgg
      121 ggccgatgaa caggcccgag aacagatagg acagcgccgg atgccgctgc cagtagatca
      181 cgaagctttt cagcagatcg ggccggcgca caaagggcga atcgagcagg ctgggcccgc
      241 ccagcacgat gtggttgccg ccgccggtgc ccatcggccg gccatcgacc atgaagcggt
      301 cggccgtcag ccccacctcg cgcgcggcct gatagagcgt ctcggtaatt tccacctgct
      361 cggcccagct ggcggcgggg tggatgttca cctcgatcac gccggggtcg ggcgttacct
      421 tcagcacggc caggcgcgga tcgggcggcg gcggatagcc ttcgatgcgc accggcacgc
      481 ggatcgcctc ggccaccgat tccaccgcgg cgatcagctc cagccagtct tccagcgtcg
      541 agacaggcgg gacgaacacc gcgacatagc cgccttccgg cccgccatcc gggcccgggc
      601 gcgcctcggc ggtgatggcg gtgcgcacca cgccctcgat ctcggtgatg gtctgctcgg
      661 cgcggggctg gcggggcggc tgctgggggc cgcgcagggc cagcgcctcg cgcgtttcaa
      721 agccgcgctc ggccagctgg cggcggaaat cggccaatgg cgcgcgcggc tctgccgtgt
      781 cgcgcgggtg gatataggga taatcgcccg cggccacata gggcagcgcc cccagcggca
      841 gccgatagcc cagcgccgaa tcgcccggca ccgcatagag cagcccccgg cgcacccgcc
      901 acacctcgct catccagcgc ggcgcctcat tggccttctg ccagcgctgc accggcagca
      961 catggcccac aggcttggtg aggccgcgct ggaaggtgcg catgaagcgg gcgcgctgct
     1021 cggggtcttc cagcgccgga tcgagcggcg aggtgttctc gggaagctcg gcctcctgca
     1081 cgatccactg gatcgtatcc tcatagaccg gctgcacgaa gcccggcgcc agccccaggc
     1141 ggatgccggc ctcttccagc actgcccggg cggtttctgt ggtcacgggg gcggtgggct
     1201 tgtcctcggg cctgtcctgc ggcagcaggc ccttttcggg atattcgccc gctaccagcg
     1261 tgggatcatg ccagatgggc acgccatcgc gccgccagta gagcgaatag ccccagcgcg
     1321 gcagggtttc ccccggatac cacttgccct ggccatgatg cagcaccgcg cccggcgcaa
     1381 aggccttgtg cagcttgcgg atcagccggt cggcataggc ggccttggtg gggcccacgg
     1441 cctcgctttt ccattcgggc gcgtcgaaat cgccatcggc gatgaaggtg ggctcgccgc
     1501 ccatcgtcag gcgcatgtcc tgcgcggcga tatcggcatc caccttctcg cccagcgcca
     1561 gcagcgcctc ccagcgctcg tcggtgaagg gcttggtgat gcgcaccgcc tcgcgcaccc
     1621 ggctgacatg catctcgaag tggaaatcgc tgtccacatg gtcgccgtcg agcgtggtga
     1681 agccgccctc gatgggcgtg gccgaacgat agtggggcgc tgcgcacagc gggatatgcc
     1741 cttcgccggc aaacatgccc gaggtagtgt ccagcgccac ccagccggcg ccgggaatat
     1801 agacctcgca ccaggcatgc aggtcgcaga tatcctccag cacgccctgc ggcccgtcga
     1861 tcgccagcac atcggccacc agctggatcg aatagcccga gacgaaacgc gcggcaaagc
     1921 ccagctggcg cagcagctgc accatcgtcc aggccgaatc gcggcaggat ccgctggcca
     1981 ggttcagcgt ttcctccggg ctctgcacgc cctgttccag ccggatgaca tagccgatgc
     2041 gctgctgcag cgcctggttc accatcacca ggaaatcgat cgtgcgcccg gccttgccgc
     2101 tggcgatgat ggcggcatat tcggccgcca gcgcatcgaa cagcgggcct tgcggctcgg
     2161 tctcgaaata ggcggccaga tcctgctgct gctcttcgga ataggcgaag ggcagttcct
     2221 ccacctgcgg ttccacgaag aaatcgaacg ggttgaccac agccagatcg gccagcagat
     2281 ccacctcgat gctgaaatga tcgaccggat cggggaatac cacccgcgcc tgccagttgc
     2341 cgtgcggatc ctgctgccag ttgaggaagt ggttttcggg gctgatcttc agcgaatagt
     2401 tgggaatggc cgtgcggctg tgcgcggccg ggcgcaacct tattacctgt gggcccagcc
     2461 gcacgggttt gctgtagcta tagcgggtgt ggtgatagag gccggccttg atcatggcgg
     2521 cgagtgtgcg gcaatcgctg ctgcgctgca acaccaaagg tggggcgcct cagcgcgcgg
     2581 cggcggccag cttggcgaac atgtcggcaa aggtgaggcg ggtgtcgata tgctcgtaaa
     2641 cgcgcatcgg ccgggcgtta gtgggggccg gctgccaggc gccatcggcg ccgatgcggg
     2701 gcgtggggcg ccaggtgtaa aggctcgatg cgctgtcggg ctcgaaggag gattgcagcg
     2761 cggtcagcgt caccagcggg ctgtcgccca gaatataggt ctcgccgatg ttgacatgat
     2821 agggcgccgc ctcggtcaac gcggccacat gctcgatcgc ggccagcaga tagcggccca
     2881 gcgcgcgcgc cggggccagc gctgcggcca gttcggcccg gctcaccagc atctgccggt
     2941 agacattgcg cggcacctgc cagatctcga tgtcggaacg gttgaagatc acctcggcgg
     3001 cggcggtgtc gatggtgagg ttgtattcgg catcgtggcg ctggggcagg ccgggcagca
     3061 ggtcggcatg ttccggcccg ccaatccaca ccagcaccag ccgccggccg atccgcggct
     3121 ctgccagcca ggcggtggcc agctcggtca ggctggcgcc ggcggcatag aacagcggca
     3181 gctgcgtgtc gtcgcgcagc gcctcggcga tgatcgcggc gctggctggc gtgggggcat
     3241 cggctgccat cgccgcattg gcgccggcga tgatgcgcgg cacgcgcgcc aggcgcatca
     3301 ggccggcgat ggtggccgcc ttttgcgcgg catgatcggc ctgccgggca gagggatcga
     3361 aaaactcgcc cttgtggagg tgcgagccga cgatcagcgg aatctcgacc gagggcgagg
     3421 cgaggtgatg ggccagctgg aacagcccat cgggatcgcc ggcaaaatca ttgtcgatga
     3481 taacgcgctg gcgcggggca atcggccggc cggctgccag cagcgggcca cccaggctgc
     3541 ccagcgcgcc cgcgcccagc ccggccagca catggcggcg cggcagcagc agctgcgggg
     3601 caggggcgat ggcaggagaa gggggcacag gcggcagctt tcgtcaaacg cacgagggcg
     3661 cgcagaaggc cgcgaaaata ggcagcgccg cctgccgaag caagcgatca aagccttgat
     3721 tttcaagaaa gtggtgccca ggagaggact cgaacctcca cgcccttacg agcgccgcca
     3781 cctgaagacg gtgcgtctac caattccgcc acctgggcac tttctctggc gcctggagct
     3841 ttcagcttcg agaggaagcg ttggctccgc gccgggtagg agggcgcctc taacgggccc
     3901 ccgcccccct gtcaacacct tgatcgcagg aaaatgcaca ggcgccaaaa tttgccgcgc
     3961 gggcaccggc aggcttgcgc aaacgcattg caaggcggcg gcggcctgtg caaatgggca
     4021 caaccccaag cgcaagcccg cttccccttg cgggctttcc agcacaagga ttttgccgat
     4081 ggccctccct ctttccgaaa cgagcctgca ggacaagctg gtggtgcttc tgggtggcgg
     4141 cggtttcgtc ggcacccatg tggcgcagga actgctggcg cgcggcgctc gcctgcgcat
     4201 cgccagccgc cacccggaac gcgcctatgc gctgaagacg ctgggcaacc tcggccaggt
     4261 gcagttcgcc cgctgcgacc ttgccaggcc cgacagcgtg aagcgcgtgc tggccggcgc
     4321 cgatgcggtg gtcaatctgg tgggcgcctt tgccggcaag cttgatgcga tccagggcag
     4381 cggccttggc gcgattgccg gcgccgcgct ggaagcgggc gccagcgcct ttgtgcaggt
     4441 gtcctcgctc aatgccgatg ccggctcgca ggtggcctat gcgcgcacca aggccgaggg
     4501 cgaggccgcc gtgctggccg ccttccccga ggcgacgatc ctgcgcccgt cgatcatctt
     4561 tggccagtat gatggctttg tgaaccgctt tgcccagctg gtggcgggcc tgccggtggt
     4621 gccggtgttc gtgcccggcg cgcagctgca gccggtgttc gtcgatgatg tggccgaggc
     4681 cgtggcgatc accctggccg atcctgaaac ccatggcggc aagacctacg agctggccgg
     4741 ccccgaaacc gtttcgatgc aggcggtgat ggagcgcatt gccacggcct gcgggcgcga
     4801 tccgctgctg ctgccggtgt gcaacctggg cgcgggcctt ttcgccgcgc ttccgggcac
     4861 gccgctcagc accgatcagc tgcacctgct gaaggccggc agcctagcca ccggcagcct
     4921 gccgggcctg gccgagctgg gcatcaccgc ccgcccgatg gagctgttcc tcgaccgctg
     4981 gctgatgcgc taccgcgagc acggccgctt cggcgtcaag cgcgcggcgt aagctttatg
     5041 gccatgcccg caaacgggca tggccgaggc ggcgccagcc cgcgtgagcg cgccaaacct
     5101 tacgcgttta cgtcggcgta atgggcgggg ggctggatca cttccatgcg ctctgccagc
     5161 agcgggcgga agctggggcg gcttttcagc acggaatacc aggccttggt ctgctcgtgc
     5221 ccctgccagt cgatcccgcc cagataatcg gccacggaaa tctgcgcggc ggcggccaga
     5281 tcggccaggc tcatggtggg gccggccagc caggggcgat gatcgagcag ataatcgatg
     5341 tagtagagat gctcgtgcgc cagcttcatc gcatcgcgca gcacgcgcga atcgggcgat
     5401 tggcgcagca ccagccgctt cttcatccgc tcatacagca gcgggcccac cacatcggca
     5461 tgcaggctct cgtcgaacag ggccaccagg cggcggatct cggcgcgctg ataggcggtg
     5521 ccgctgatca tcggcgcgcc gctctcggtt tcctcgatga actcgcagat cgcccggctg
     5581 tcggccagca cggcgccctt ggcggcatgg cgcagcaccg gcgtgtggcc ggccgggttc
     5641 atggccagga aggcgtcgcg ccgctcccac gggttctcgc gccagagttc ataggcgatg
     5701 cccttctcgc tcagcagcag gcgcagcttg cggctgaagg ggcacagggg gaactgatag
     5761 agctgccaca tgccgatgat ccttgcaccg gaaggggggc gcacgtccac ccgcggcggc
     5821 aaaggggcag cgcaaatttg gggatttagc ctggctgcgc cccatcatgg gccagcagca
     5881 cgctgtcgcc gcgcgccagc acccacaggc tgaggcccgc cgcctgcgcc cgcgccaccg
     5941 ccagcgaggt gggcagcgag atcgtcacca gcgtgtgggc accggcgcgc accgccttct
     6001 cgacgatctc gtagctgcag cgcgcggtgg aaagcacgaa gcccgccgcc atatccgccc
     6061 cggcccgcgc cattgccccc accagcttgt cgagcgcatt gtgccggccc acatcctcgc
     6121 gccaggccac aagggtgcca tcggccgcgc agaaggcggc ggcatgggcc gcgcccgtgg
     6181 cccgccccag cgcctgcacc gggcccagcg ctgccagcgc ggcgaagatg gcgccgcttt
     6241 ccagccgggc atggggcggc accggcggca agggcttggc cagcgccgcc agattttcca
     6301 ctccgcacag gccgcagctt gattcggcca cgcgccggcg cacccgctca cccagctgct
     6361 ctgcccccag cgtcgggatg gtggcgcgga tgatccagcc cagctccacc tcggccacgg
     6421 cgatatcggc aatggcccgg gcggtgggcg ccagcccctc ggccagggca aagccggtgg
     6481 caaaatcagc cagatccaca ggcgttgcca tcatcaccgc ataggccagg ccgttgcatt
     6541 ccagcgccac cggcgcttcg ggcaccagcg cgcgccgcgc ggcaccccgc ccgccatcgg
     6601 ccgccagcgc cggccaatcg gcctgcggca gcggcggcag cggctctggc ggggcaatcg
     6661 ctgtcatcag gccgggcgat gcagggcgca cagcttgttg ccgtcggggt cgcgcagata
     6721 ggccagatac aggccgttgg ggcgctggcc gggggcatcc tcgatcgccg tgccgccatg
     6781 ggcaacgccg gcggcatgcc aggcgttcac ctgctcctcg ctggccatgc caaagccgat
     6841 ggtgccgcca ttggcgaccg tggcgggctc tccgttgatc ggccggcaga cgatgaacag
     6901 cgcgccggca tggcgataga cgatgcggcc gatctgctcg tcgatcagcc cggtggagcc
     6961 atcgagagcg gcgaacaggg catcgtaaaa gatcttggcg cgggcaagat cgttcgatcc
     7021 cacaaaaaca tggctgaaca tggggagggt cctttcggca ttgcagcttg gttttgcggc
     7081 cgaaccataa tcaccgggca gggcgcggca agcaggatat gccgcaagcc gccgtccagc
     7141 cgccgcccac aggaaaaggg ccgccccgca gggggcagcc ctttccttcg ctgttcagct
     7201 gtgtggcgga aggctcagcg cggcagttcg ctggcgccca tcagcgcctc gtccaccgcg
     7261 cgggcggcct gccggccctc acggatcgcc cagaccacca gcgactggcc gcggcgcatg
     7321 tcgccggcgg caaagatctt gggatcgctg gtgcgatagt cttccgtatt ggcctgcaca
     7381 ttgccgcgcc catccagcgc cacgcccgac tggtcgatca gcccggcctt cttggggccg
     7441 acaaagccca tcgccagcag gatgagatcg gccttcagcg tgaaggtgct gcccggcact
     7501 tcggtcatct tgccgccggc ccattcgaca tgggtgcatt ccagccccgt gacgacgcca
     7561 ttctcgccga tcacccgctt ggtcagcacg gcaaagtcgc gcgtcacgcc ttcctcgtgg
     7621 ctggtggagg tgcgcagctt cagcggccag ttcggccagg tcagcgcctt gtcctccttc
     7681 accggcggct cgggcatgat ctccagctgg gtgacagaga gcgctccctg gcggttcgaa
     7741 gtgcccacgc agtccgagcc ggtgtcgccg ccgccgatga cgatgacatg cttgccggtc
     7801 gccgtcaggc tgccgcgcgg tgcggcgcgg atttcatcat cgccggcatt gcgcttgttc
     7861 tgctgggtga ggaattccat ggcaaagcgc acgcccgcca gctcgaagcc ggggatgccc
     7921 agcgggcgcg gttcctcggc gccgccggtc agcaccacgg cgtcgaaatt gtccttcagc
     7981 gaggccaccg acacgctcac gcccacttcc gtcgaggtgc ggaaggagac gccctcggcc
     8041 tccatctgca ccatgcggcg gttgatgagg tgcttttcca tcttgaagtc ggggatgcca
     8101 tagcgcagca gcccgcccac ccggtcgctc ttctcgaaca ccgttacgga atggccggcg
     8161 cgggccagct gctgggcgca ggccagaccc gccgggccgg aacccaccac ggccaccgcc
     8221 ttgccggtct tgtgggcggg cacctgggga gtgatccagc cctcttccca gcccttgtcg
     8281 acaatcgcgc attcgatcga cttgatcgtc acggcctggt cgatgatgtt cagcgtgcag
     8341 gccgcctcgc aaggggcggg gcagatgcgg ccggtgaact cggggaagtt gttggtggaa
     8401 tgcagcactt ccagcgcgcc gcgccagttg ccttcatagg tcaggtggtt ccagtccggg
     8461 atgatgttgt tcaccggaca gccgttgtga caatagggaa tgccgcaatt catgcagcgc
     8521 gaggcctgcg ccttcagcgc cccctcggca tgcggaatga cgaattcctt gtagtggtgg
     8581 atgcgctcct tcgggtccgc gtaggtgcgg tcctgacggt caagctcgag aaagccggtt
     8641 tccttgccca tgataatcta acccttttat cccgaactct tattccgcgg cctcggtggc
     8701 agcggcctgg ttttccgcgt ccatcgcctg cagcgccttc gcataatcgc gcggcatcac
     8761 cttcacaaac ttgcccagcg tgttggccca gtcgtccagc agggcgcggg cccgggcaga
     8821 gccgctgtag aggtggtggc gttccagcag gatgcgcagg cgctcggcat cgtggcgcag
     8881 catgtcgccc atgcccagat cctgcaccga gatggcgcgc tgctgcggcc ggccggcccc
     8941 gtcttcctcg tcacgctcgg ccgaaacggg cagcagatca acctgtgcct gattggccag
     9001 cttgtcgaaa ttgccagcct cgtcatagac ataggccacg ccgcccgaca tccctgcggc
     9061 aaagttgcgg ccggtcttgc ccagcaccac aaccacgccg ccggtcatat attcgcagcc
     9121 atggtcgccc gtgccttcca ccaccgcgat ggcgccggaa ttgcgcaccg cgaagcgctc
     9181 gccagccaca ccggcaaaga aggcctcgcc gctgatcgcg ccatacagca ccgtgttgcc
     9241 cacgatgatg ttctcggtcg gctcgcgctg cacgaaggca ggttgccgca cgatgacccg
     9301 gccgcccgaa agccccttgc cgacatagtc attgccatcg cccgtcagct ccagcgtcac
     9361 gccatgggcg aggaaggcgc cgaagctctg gccggcggtg ccggtcaggc tgatgtggat
     9421 ggtgttgtcg ggcaggccgg catggccata gcgcttggcc acctcgcccg agagcatcgc
     9481 gcccaccgtg cggttgacgt tgatcacccg gcgctcgatg cgcacgggct tgctttccac
     9541 cagcgcatcg gcggccgccg cgatcagctc gttgtcgagc gcgccatcca ggccatggtc
     9601 ctgcgtctgg ctccagttca ggctggggct ggcatcgccc ggcacctgat acagcagctt
     9661 ggtcaggtcg atcccctgcg ccttccagtg gttcacggcc ttcttcggct ccagccggtc
     9721 cacccggccc accatctcgg caatggtgcg gaagcccatc tcggccatga tcgcccgcag
     9781 ctcttcggcg acgaagaaga agtagttgat cacatgctcg ggctggccgg tgaagcgggc
     9841 gcgcagcaca gggtcctgcg tggcaacgcc caccgggcag gtgttgagat ggcacttgcg
     9901 catcatgatg cagccggccg cgatcagcgg ggcggtggca aagccaaact cgtcggcgcc
     9961 cagcagcgcg gccacggcca cgtcgcggcc ggtgcgcagg ccgccatcgg cctgcacgca
    10021 gatgcggctg cgcaggttgt tcagcagcag cgtctgctgg gtttcggcca ggccgatttc
    10081 ccacgggctg cccgcatgcg tcaggctggt cagcggcgag gcaccggtgc ccccctcata
    10141 gcccgagatc gtcacatggt cggcccgcgc cttggaaacg ccggccgcca ccgtgcccac
    10201 gcccacttct gacaccagct tcaccgagat gcgcgcgccg gtgttcacgt tcttcagatc
    10261 gtgaatgagc tgggcgagat cctcgatcga atagatgtcg tggtgcggcg gcgggctgat
    10321 caggcccacg ccgggggtgg aatggcgggt cttgccgatg gtcttgtcaa ccttgtcgcc
    10381 cggcagctgg ccgccttcgc cgggcttggc gccctgggcc atcttgatct ggatgtcgtc
    10441 ggcattgacg agatattccg ccgtcacgcc aaagcggccc gatgccacct gcttgatcga
    10501 cgaacgcagc gaatcgccat tgggcagcgc cacgaagcgc ttgggatcct cgccgccttc
    10561 acccgtgttc gacttgccgc cgatgcggtt catcgcgatc gccagcgtgg tgtgcgcttc
    10621 ccagctgatc gagccatagc tcatcgcgcc ggtggcaaag cgcttgacga tctcgctcgc
    10681 cggctcgacc tcgctgatat ccagcggcct gtcgcccttc ttcagctcga tcagcccgcg
    10741 gatggtcagg ttgcgggtcg cctgatcgtt gatcgtctcg gcaaattcct tgtatttttc
    10801 aggaatgttg ccgcgcacgg catgctgcag gctggcgatg ttgcccggcg tccaggcgtg
    10861 ttcctcgccg cgcaggcgat agccatagat gccgcccaca tcgagcatgt gcttgtagat
    10921 cgggttgtcg ccataggccg cggcgtggcg gcgcacggtt tcctcggcca cttccttcag
    10981 gccggcgccc tcgatcgtgg tggccgtgcc cgagaaatac ttctcgacga aggcgctgga
    11041 gaggcccacc gcatcgaaga tctgcgcgcc gcaataggat tgataggtgc tgatgcccat
    11101 cttggacatc accttgagga tgcccttgcc gatcgccttg atgtagttct tctgcacctc
    11161 ataggcgctg agcggcagtt ccttgcgctc gcggatatct tccagcgtct cgaaggcgac
    11221 ataggggttg accgcttcgg cgccatagcc ggccagcacg cagaagtgat gcacctcgcg
    11281 cgcctcgccg gtttccacca ccaggccggt ctgcatgcgc aggccctggc gcaccagatg
    11341 gtggtgcacc gcagccgttg ccagcgcggc cggcatgggg atgcgatccg ggccctgggc
    11401 gcggtccgac aggatcagga tgttcttgtc ggccagcacc gcttcggtgg ccgcccacac
    11461 catttccttg atcgcctgct ccagcccttc ggcgccgctg gcggcatccc aggtggtgtc
    11521 gatcgtggcg gtgcggaagg cgccatcgag cgctgcctcc acgctgcgga tcttggcgac
    11581 atcggcattg gtgaggatcg gctggtcgat ctccaggcgc ttgtggctgc cggcatcatg
    11641 gcccagcagg ttggggcgcg ggccgatcat gctgaccagg ctcatcacca gctcctcgcg
    11701 gatcgggtcg atcggcgggt tggtcacctg ggcgaagttc tgcttgaaat aatcgaacag
    11761 caggcgcgac ttgcgcgaca gcaccgcgat cggcgtgtcg gtg
//
LOCUS       DASRYE010000121        14467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_217990,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000121 DASRYE010000000
VERSION     DASRYE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14467
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217990"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1401)
                     /locus_tag="VFF98_06835"
     CDS             complement(169..1401)
                     /locus_tag="VFF98_06835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZU63378.1"
                     /translation="MAGRDFRRQGHRATRRVGVAAAGLIMAMAGPGAAHEPVRQEVFG
                     TIVFYHQAVCAHDAPAGTAYCHAHMRTDAQGRTMAGKPAVTSAAPQGLAPADLRSAYR
                     LTANGSSATIVAVVDAYGYANAEADLAVYRAQYGLPACTTANGCFIKLNQAGVAGSYP
                     AGNVGWQQETALDLDIVSAMCPNCKIMLVEATTASFANLAAAVNTAVAKGAKVVSNSY
                     GGSETGSSSYAAAYNHPGIAITVSAGDSGYGAQFPATAPGVIAVGGTSLARAGNSRGW
                     SETVWQGTGSGCSALYAKPAWQTDKLCARRMEVDIAAVGNPYTGAAVYAPNSSGSGSG
                     WQVYGGTSLSAPLIGGIYGALGAGPTAAQKIWGTTSGLNDITSGNNGRCSGTYFCTAG
                     TGYDGPSGMGTPAGSAGF"
     gene            1641..2375
                     /locus_tag="VFF98_06840"
     CDS             1641..2375
                     /locus_tag="VFF98_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63379.1"
                     /translation="MAHPSLAPPCPAQPPLADTAAAGPPRKLTPRQEERRRRILAVAQ
                     AQISELGYEAMTMRGIAAASGTAEKTLYNIFGTKDRLVAQAARYRSGNAFAEAARRAG
                     QPGWDLLLAFCDVASEWTQETPILSRGLALVLADHAELVGLGQIYETYVGASLRAMMA
                     GGMLAPAMPLAVVVRVVRLAVVATVVFWARGELSDAELRAYLRLRMAESLLPHAAPGG
                     GAIIACAQGATRQLADLAASAPAMEG"
     gene            2550..5009
                     /locus_tag="VFF98_06845"
     CDS             2550..5009
                     /locus_tag="VFF98_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63380.1"
                     /translation="MSPIDTTVAPTAQRRLRPVALRLALGASLLPALCTAAQAQTAEA
                     PAPAPAHATGGLEDIIVTARRKVENLQDVPVSVTAISAQQIARYDMTSLEKISTQTPQ
                     FTIGRASNGSGAQLTLRGIGSSSTSVGIEQSVAVILDGVYYGQGRVINEGFLDLAGVE
                     MLKGPQALFFGKNATAGVISLKTADPTSRPEYTAKLGYELNGKDLVGEFVGSGPLSDD
                     LGIRVALRASKMFGYYFGNAGLNETYSTTDMSTGTTTAHAAPAFKGNNPGTREFLGRV
                     TLKYKPTDRLTMTLKANASANDDDNNSWNYVAYGCANPNGTFGTNPALKCTHSFTTYQ
                     NYFPANLAGTNPYSNPNGDLYNSYRSWALTGTMDYNFDHLALTWVNNYNKNVNQWACD
                     CTFVSAPDPLLAPSTEHSSYHAFSSELRAQTKFDSPVNLMMGVLYQSTKRNHTQTGSF
                     GGVSDSSAPAAFQYLGYYKHSETNGETISLYSQLNWKIVPGVEAAGGVRYTHETKDSF
                     LVQPYVNSGLQSLFLQNVLIPAHQMWNNWSPEATLTWKPVRDITVYGAYKTAYKSGGF
                     SNSGFVSASTTVNDLAFNPETAAGFELGVKTTLFDHQLRFNVDIYSYLYKNLQVDFFN
                     ATTFAFITTNAGGARTKGVELEAEFAPRAMPGLNLHGSLNYNHARYSNYIAPCYGGQT
                     AAEGCGNGLTFHGADAQDLSGKPTAVAPDWTGSFGFGYEAAVSARHKLGFNMDARYSS
                     SYLASSFGKPNSLQEQYLTLDATLRLKTLDDAYEFAIIAKNITNRFIVGGAVDAPNSG
                     ADTIGFVNMPRTVQLMATVHF"
     gene            5121..5876
                     /locus_tag="VFF98_06850"
     CDS             5121..5876
                     /locus_tag="VFF98_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63381.1"
                     /translation="MLSGKIALVTGAASQAGLGFATARKLARAGAQVWLSDLAGAAAT
                     ERAAALCAEGLAAHALPHDVTSEASWAEVLGAITAESGGLDVLVNNAGIAVLHATPEL
                     TPMDWQRQIDVNLTSVYLGCRAALPLLQARGGGAIINLSSVAGLVGIAGASAYAASKG
                     GVRLYSKSLAIEVARTGVRVNSVHPGVIWTEMQQVAIRDNPAQYDAINAAIPMGRMGE
                     PDDIAEMITFLAGDGARYITGAEFVVDGGLTAQ"
     gene            complement(5925..6116)
                     /locus_tag="VFF98_06855"
     CDS             complement(5925..6116)
                     /locus_tag="VFF98_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HZU63382.1"
                     /translation="MNQQEKNAAIPAAALDALGHLEESLASVRAALQDLRYGSVALTV
                     HEGRVVQIDITEKKRFRPN"
     gene            complement(6113..7096)
                     /gene="cysA"
                     /locus_tag="VFF98_06860"
     CDS             complement(6113..7096)
                     /gene="cysA"
                     /locus_tag="VFF98_06860"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HZU63383.1"
                     /translation="MIELNHIAKRFGDYPALHGVDLAIRPGEFIALLGPSGSGKTTLL
                     RIIAGLEQQEEGRVLFDGEDVSARPVAERGVGFVFQHYALFRHMSVAENVGFGLSVRK
                     GRLGLNRRQIREKAEELLKLVQLDGLGDRYPSQLSGGQRQRVALARALAIQPRLLLLD
                     EPFGALDARVRKDLRRALRDLHNRVGLTSVFVTHDQEEALELADRVVVMNKGHIEQVG
                     TPEEIYAHPATPFVSAFVGETNLLPGTAGDLHVRPHELAIVAEGPGASAVRVSNVFRK
                     GGLWRIEGALADGTRLVEIDAAADAVLPQPGAVIHVRAQTSRSFEEEGATS"
     gene            complement(7093..7941)
                     /gene="cysW"
                     /locus_tag="VFF98_06865"
     CDS             complement(7093..7941)
                     /gene="cysW"
                     /locus_tag="VFF98_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZU63384.1"
                     /translation="MSAAASRATTEAPFIRWLLIGLALAWLVLLLGLPLAAVFAEALR
                     DGWAAFRAAIVTPDALAAIRLTLIVAAISVPLNTLFGLAASWAIAKFEFTGKSLLITL
                     IDLPFSVSPVVSGLIFVLIFGAQGVFGEWFIDHDIKVIFAVPGIVLATVFITFPFVAR
                     ELIPLMQEQGRSDEEAALSLGANGWQTFWHVTLPNIRWGLLYGVLLCNARAMGEFGAV
                     SVVSGHIRGETNTMPLHVEILYNEYDFVGAFAVASLLAGLALVTLALKTALEWRFDLG
                     NKGARH"
     gene            complement(7938..8807)
                     /gene="cysT"
                     /locus_tag="VFF98_06870"
     CDS             complement(7938..8807)
                     /gene="cysT"
                     /locus_tag="VFF98_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZU63385.1"
                     /translation="MTQTSPPLPAGRRRARARKARSVLPGFGLSLGLTVGWLTLIVLI
                     PLSTLFWKTAGLGFDRFLEVGFSPRALAAYRLSLTTAALAGLANAAAGLLVAWVLVRY
                     SFPAKRLIGAAVDLPFALPTAVAGIALTTLFTPNGWVGRYLDPLGIKVAFTPLGITIA
                     LVFIGLPFVVRQVEPVLADLGRDVEEAAATLGASRWQTFVRVILPSLAPALLTGFALA
                     FARGVGEYGSVIFISGNMPGKTEIAPLLIVTQLEQFAYASATAIAMILMAASFALLLL
                     INLLQAYSRRHEA"
     gene            complement(8876..9898)
                     /locus_tag="VFF98_06875"
     CDS             complement(8876..9898)
                     /locus_tag="VFF98_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZU63386.1"
                     /translation="MKRRSILAGSLAGLAIAAAGFAAAPALAGPPPAQITNVSYDPTR
                     ELYEAINPAFAEWWKKRTGQDVTFRTSHGGSGAQSRAILDGLEADVATLGLAYDIDVL
                     ADKRQLLSPDWQKKLAYNSAPYTSTIVFLVRKGNPKGIHDWADLVKPGVQVITPNPKT
                     SSGARWNFLAAWTYGRKHGGDGAARSYVQQLYAHVPVLDSGARGSTNTFVERGIGDVL
                     IGWENEAFLATNKLGRDKFEIVVPSVSILAEPTVAVLTKNTEKHHTTAVANAYLNFLY
                     SPWAQDIIAKNYYRPRDPKVAKRWGVQFPQLQLSQVDRDYGGWRKAQKTYFDDGGIYD
                     QIASAR"
     gene            complement(10225..12159)
                     /locus_tag="VFF98_06880"
     CDS             complement(10225..12159)
                     /locus_tag="VFF98_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HZU63387.1"
                     /translation="MIRRILSLALALLAALLLPAGAALAQPGGPDDAGGEQRGISLPP
                     MAAPAPHAPRAVPVHPIADLAPLAPAVGCASLAALDISRQVMAKTTLAAEEVAGTDSA
                     QGYCKVSGTIFPQVHFELRLPLKGWTQRYLQTGCGGLCGMLRIDAPQAESCAPVTDGS
                     VAIASTDMGHQGMDAAWGKSLAQRQDFADRSLHETAVAAKALIRAFYGKSVRYAYFAG
                     CSDGGREALIEAQRFPGDFDGILAGAPALHFAYQNSFHHAWLAAANTGADGKPILLAA
                     DMAPLHAAVLKACDGLDGLEDGQITDPRACHFDPAVVQCAGAYQAGQCLTAQQVAAVR
                     KVYAGAHTADGKALEAGALMPGSELNWVGVFVPRSADQPVFSGIIALSSINHLLFAPD
                     AAPAYTIANFSFTEEMARKLEAARAFYDADNADLSLFTGHGGKLILYHGWADPHISPL
                     STLDYFERVGARLGADKRDAALRLFLLPGMGHCSGGDGPSAAPLLEELMAWVEKGSAP
                     QAVEAHRASQGRAGQPGGMRGMAGGRPGEGRHWGPPSGAGGQFGPPPGADGQMPPPPE
                     GARHAQGFGPGGPGGKPGEMGPPPGLEPDAVQLPPRARPVYAWPQVAVYKGQGPVDEA
                     TSFTAGAAAPAGLIDWLGAR"
     gene            complement(12277..13161)
                     /locus_tag="VFF98_06885"
     CDS             complement(12277..13161)
                     /locus_tag="VFF98_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HZU63388.1"
                     /translation="MRDRASLRGRARLRAWAVACRYGLPARLGGLLPAPTAAVAARWA
                     AARVALGQRVTLGFFHRADMPPAAIASANLAAVRALAGVGAAAPLLAVKAPPLGFDAG
                     LLAELAAEAGACGVGLVFDAHAAHHADATLAAMEKLAGGPSATGAPVPLGLALPARWR
                     RSLADARRLRDMPVAIRLVKGEWADPAGEDGHVTRRFMALAGFLAGRAAPVAVASHDA
                     DLAACALDLLQEGATPCELEQLRGLPMRQTAGVARARGVPLRTYLPFGPGWWPYALER
                     ALARPGLGLWFLKDWLAA"
     gene            complement(13161..14312)
                     /locus_tag="VFF98_06890"
     CDS             complement(13161..14312)
                     /locus_tag="VFF98_06890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU63389.1"
                     /translation="MSQAQGSAGALVPGAMAARPARPVLRAVPLADMAAQPDMLAGWR
                     QLAEAGCLPMQGPAFLLDVARCWLADADARALVVEAGGRLRALLPLVRERGWLARWRM
                     IGPCEVYEPNDALYADAAAAAALAEGLARRRLALRLHRVPAGSALVPALRRALRGRGW
                     LVARAAAPTPAIALHAGWADPASQLNPGRRSDLRRAARRAAACGVVSYEMLCPGPARF
                     DALFDEAVAVEQSGWKRAAGSAMRCDPAKAAFFRGWMRQACDEGLGRFAFLRIDGRAV
                     AMQMGAMWQGRYWLFKIGYDEAHCACSPGNLLMLHCLGEAARAGLSSFELLGGMERWI
                     ADLWTRAANPCLDIRIYPFAPAGLAALLADGAAWAGQKLRARATGPGPG"
BASE COUNT         2512 a         5175 c         4635 g         2145 t
ORIGIN      
        1 cgggtttcgc cgggcgtgtc ggccagcagg cgcacatcgg tcagcacggc gggcactggc
       61 tgcgggcgca gatcctccgc catcgcccgc gccggcagca gcaccgccag ccacagggcg
      121 aacagcgccg ccagccccct catttttgcc cccacgcgca gatgccccct agaagccggc
      181 cgatcccgcc ggcgtgccca tgcccgatgg gccatcatag cccgttccgg ctgtgcagaa
      241 ataggtgccg ctgcagcgcc cgttgttgcc gctggtgata tcgttgagcc cgctggtggt
      301 gccccagatc ttctgcgcgg cggtgggccc tgcgcccagc gcgccataga tcccgccgat
      361 cagcggggcc gacaggctgg tgccgccata gacctgccag ccagagccgg acccgctact
      421 gttgggcgca tagacggcag cgccggtgta ggggttgccc acggccgcaa tatccacttc
      481 catgcgccgg gcgcacagct tgtcggtctg ccaggcgggc ttggcataga gcgcgctgca
      541 accgctgccg gtgccctgcc acaccgtctc gctccagccg cggctgttgc cggccctggc
      601 aaggctggtg ccgcccaccg cgatcacgcc cggcgccgtg gccggaaatt gcgcgccata
      661 gccgctgtcg cccgcgctga cggtgatggc gatgccgggg tggttatagg ccgcggcata
      721 gctgctgctg ccggtttcgc tgccgccata gctgttggag acaaccttgg cccccttggc
      781 caccgccgtg ttcaccgccg ctgccagatt ggcaaagctg gcggtggtcg cctccaccag
      841 catgatcttg caattggggc acatggcgct gacgatatcg agatcgagcg cggtttcctg
      901 ctgccagccg acattgcctg ccggatagct gcccgccacg cccgcctggt tcagcttgat
      961 gaagcagcca ttggccgtgg tgcaggccgg caggccatat tgcgcgcgat agaccgccag
     1021 atccgcctcg gcattggcat agccataggc atcgaccacc gcgacgatcg tggcggacga
     1081 gccattggcg gtcagcctgt aggcgctgcg cagatcggcc ggggccaggc cctgcggcgc
     1141 cgcgctggta acggcgggct tgccggccat cgtccgcccc tgggcatcgg tgcgcatatg
     1201 ggcgtggcaa taggccgtgc cggccggcgc atcatgggcg cacacggcct gatgatagaa
     1261 cacgatagtg ccgaaaacct cctggcgcac cggctcgtgg gcggcgccag ggccggccat
     1321 ggccatgatc aggcccgccg ccgccacgcc cacacggcgc gtggctctat gcccctggcg
     1381 ccgaaaatcc cgtcctgcca tcttcgccat cccgaaaagc ggtccttgcc ggcagcatgg
     1441 ggcttttcgg tttttttccg gttagcagcc cccaccgcag ttgccgctgc tataggatgg
     1501 ccgggcggca ggcgccagcg cctttggcct gcccctgcca gaaccggccc cggcaccata
     1561 aaccggcttg gcaagcccgg cccggcagcg catgcacagc cgccatgccc gatcacccgc
     1621 ctgcccctgc agatccttct ttggcccatc cttccctggc ccccccttgc ccggcccagc
     1681 cgccccttgc cgatacggcc gccgccgggc cgccgcgcaa gctcaccccc cggcaggaag
     1741 aacgccgccg ccgcatcctg gccgtggcgc aggcgcagat ctccgaactg ggctatgaag
     1801 cgatgaccat gcgcggcatt gccgcggcca gcggcaccgc cgaaaaaacc ctctacaaca
     1861 tctttggcac caaggaccgg ctggtggcgc aggcggcccg ctatcgcagc ggcaacgcct
     1921 ttgccgaggc ggcccggcgc gccggccagc ccggctggga tctgctgctg gccttttgcg
     1981 atgtggccag cgaatggacg caggaaaccc caatcctctc gcgcgggctg gcgctggtgc
     2041 tggccgatca tgccgaactg gtggggctgg ggcagatcta cgagacctat gtgggcgcca
     2101 gcctcagggc catgatggcc ggtggcatgc tggcgccggc catgccgctg gcggtggtgg
     2161 tgcgggtggt gcggctggcg gtggtggcaa cggtggtgtt ctgggcgcgg ggcgaactgt
     2221 ctgacgcgga actgcgcgcc tatctgcggc tgcgcatggc cgaaagcctg ctgccccatg
     2281 ccgcgcccgg cggcggggcg atcatcgcct gcgcgcaagg ggccacgcgc cagctggccg
     2341 atctggcggc atcggcgccg gcgatggagg gctgagcgca ccctctcact ttttacagca
     2401 cgctataaaa atatttgacg caaagccccg tctgcggttc tctcctgccc agaacaacag
     2461 gtcgggaggg gggctcggat cgtgcggcgt ggcggccatc accgctggcg cagcaccgtc
     2521 acctctcctg ccaggaggag aggaaaacca tgtcaccgat cgacaccacc gttgcgccca
     2581 cagcgcagcg ccgcctgcgc cctgtggccc tgcggctggc gctgggcgcc tcgctgctgc
     2641 cggcgctctg caccgccgcc caggcgcaga ccgcagaggc ccccgccccc gcccccgcgc
     2701 acgccaccgg cggcctggag gacatcatcg tcaccgcccg ccgcaaggtg gaaaacctgc
     2761 aggatgtgcc cgtctccgtc accgcgatca gcgcccagca aatcgcccgc tacgacatga
     2821 ccagcctgga gaagatctcc acccagacgc cgcaattcac catcgggcgc gcctcgaacg
     2881 gctctggcgc gcagctcacc ttgcgcggca tcggctcctc ctccacctcg gtgggcattg
     2941 aacagtcggt cgcggtcatt ctcgatggcg tctattatgg ccagggccgc gtcatcaacg
     3001 agggcttcct cgatctggcc ggcgttgaaa tgctgaaagg cccgcaggcg ctgttcttcg
     3061 gcaagaacgc cacggccggc gtgatctcgc tgaagaccgc cgatcccacc tcgcgcccgg
     3121 aatataccgc caagctgggc tatgagctga acggcaagga tctggttggc gaattcgtcg
     3181 gttcgggccc cttgagcgat gatctgggca ttcgcgtggc gctgcgcgcc tccaagatgt
     3241 tcggctatta tttcggcaat gccggcctca acgaaaccta ttccaccacc gacatgagca
     3301 ccggcaccac caccgcccat gccgcccccg ccttcaaggg caacaacccg ggcacgcgcg
     3361 aattcctcgg ccgcgtaacg ctgaaataca agcccaccga ccggctgacg atgacgctga
     3421 aggccaatgc ctcggccaat gacgatgaca acaacagctg gaactatgtg gcctatggct
     3481 gcgccaaccc caatggcacc ttcggcacca atccggcgct gaaatgcacc cacagcttca
     3541 ctacctacca gaactatttc cccgccaacc tggccggcac caacccctat tccaatccca
     3601 atggcgatct ctacaattcc tatcgcagct gggcgctgac cggcacgatg gattacaatt
     3661 tcgaccatct ggccctcacc tgggtcaaca actacaacaa gaacgtcaac cagtgggcct
     3721 gcgattgcac ctttgtgtcg gcgcccgatc cgctgctggc gccttccacc gaacattcct
     3781 cctatcacgc cttctcgtcc gaactgcgcg cgcagaccaa gttcgacagc ccggtcaacc
     3841 tgatgatggg cgtgctctac cagagcacca agcgcaacca cacgcagacc ggctccttcg
     3901 gcggcgtatc agacagcagc gcgccggccg ccttccagta tctgggctat tacaagcatt
     3961 cggaaaccaa tggcgagacg atctcgctct acagccagct gaactggaag atcgtgccgg
     4021 gcgtggaagc ggccggcggc gtgcgctaca cgcatgagac gaaggacagc ttcctggtgc
     4081 agccctatgt caattccggg ctgcagagcc tgttcctgca gaatgtgctg atcccggccc
     4141 accagatgtg gaacaactgg tcgcccgagg ccaccctcac ctggaagccc gtgcgcgaca
     4201 tcaccgtcta tggcgcctac aagaccgcct ataaatccgg cggcttctca aattccggct
     4261 ttgtcagcgc cagcaccacg gtgaacgatc tggccttcaa ccccgaaacc gccgccggct
     4321 tcgaactggg cgtgaagacc accctgttcg accaccagct gcgcttcaac gtcgatatct
     4381 acagctatct ctacaagaac ctgcaggtgg atttcttcaa cgccaccacc tttgccttca
     4441 tcaccaccaa tgccggcggc gcgcgcacca agggcgtgga gctggaagcc gaatttgccc
     4501 cccgcgcgat gccggggctg aacctgcatg gctcgctgaa ctataatcac gcgcgctaca
     4561 gcaactatat cgccccctgc tacggcgggc agacggcggc cgaaggctgc ggcaacggcc
     4621 tcaccttcca tggcgccgat gcgcaggatc tctccggcaa gcccaccgcc gtcgcgcccg
     4681 actggaccgg ctccttcggc tttggctacg aggcggcggt gtcggcccgc cacaagctgg
     4741 gcttcaacat ggatgcgcgc tattccagct cctatctggc ctcgtccttc ggcaagccca
     4801 attcgctgca ggaacaatat ctgacgctgg atgccaccct gcgcctgaag acgctggacg
     4861 atgcctatga gttcgccatc atcgccaaga acatcaccaa ccgcttcatc gttggcggcg
     4921 cggtggatgc acccaatagc ggggccgaca cgatcggctt cgtcaacatg ccgcgcacgg
     4981 tgcagctgat ggccactgtg cacttctgac cttttgcctt cctccctgtg ccgtggcggg
     5041 gccatcacgt ttctccccgc cacggttttt ttcttttccg gcagatgatt gctgcccttt
     5101 cttcaaggag tttcccgcat atgctgtccg gcaagatcgc gcttgtcacc ggtgctgcct
     5161 cgcaggcagg tctgggcttt gccactgccc gcaagctggc ccgcgccggc gcccaggtgt
     5221 ggctgagcga ccttgccggg gccgctgcaa ccgagcgcgc cgccgcatta tgcgccgaag
     5281 gcctggccgc ccatgccctg ccccatgatg tgaccagcga ggccagctgg gccgaggtgc
     5341 tgggcgccat caccgccgag agcggcgggc tggatgtgct ggtcaacaat gccggcatcg
     5401 ccgtgctgca cgccacgcca gagctgacgc cgatggactg gcagcgccag atcgatgtca
     5461 acctcaccag cgtctatctg ggctgccggg cggccctgcc gctgctgcag gcgcgcggcg
     5521 gcggggcgat catcaacctg tcgtcggtgg cagggctggt ggggattgcc ggcgcctcgg
     5581 cctatgcggc cagcaagggc ggcgtgcggc tctacagcaa atcgctggcg atcgaggtgg
     5641 cgcgcacagg ggttcgcgtc aattccgtcc accccggcgt gatctggacc gagatgcagc
     5701 aggtggccat ccgcgacaac cccgcgcaat atgacgcgat caacgccgcc atcccgatgg
     5761 gccgcatggg cgagcccgac gatatcgccg agatgatcac ctttctggcc ggcgatggcg
     5821 cgcgctatat caccggggcg gaattcgtcg tcgatggcgg gctgacggcg caataaggcc
     5881 gagggccgct gggggcgcac attccccctg gcggcccctc accgtcagtt cggccggaag
     5941 cgcttcttct cggtaatgtc gatctgcacc acccggccct cgtgcaccgt cagcgccacg
     6001 ctgccatagc gcagatcctg caaggccgcc cgcacgctgg ccaggctctc ttccagatgg
     6061 cccagcgcat ccaatgcggc ggcagggata gcggcattct tttcctgctg gttcatgatg
     6121 ttgctccctc ttcctcgaaa ctgcgcgatg tttgggcgcg cacatggatc accgcgcccg
     6181 gctgcggcaa cacggcatcg gccgcggcat cgatctccac cagccgggtg ccatcggcca
     6241 gcgccccttc aatccgccac agcccgccct tgcggaacac attgctgacc cgcaccgcgc
     6301 tggcccccgg cccttcggcc acgatcgcca gctcgtgcgg gcgcacgtgg agatcgcccg
     6361 ccgtgcccgg caagaggttc gtctcgccca caaaggccga gacgaagggc gtggccgggt
     6421 gggcgtagat ctcctcgggc gtgcccacct gctcgatatg gcccttgttc atcaccacca
     6481 cgcggtcggc cagttccagc gcctcttcct gatcatgcgt gacgaagacc gaggtgagcc
     6541 ccacgcgatt gtgcagatcg cgcagcgccc ggcgtagatc cttgcgcacc ctcgcatcca
     6601 gcgcgccaaa cggctcgtcc agcagcagca ggcgcggctg gatggccagc gcgcgcgcca
     6661 gcgccacacg ctggcgctgc ccgcccgaca gctgggaagg atagcgatcg cccagcccgt
     6721 ccagctgcac cagcttcagc agttcctccg ccttctcgcg gatctgccgg cggttgaggc
     6781 ccagccggcc cttgcgcacg gacaggccaa agcccacgtt ttccgccacg ctcatatggc
     6841 ggaacagcgc ataatgctgg aagacgaagc ccaccccgcg ctcggccacc ggccgggccg
     6901 aaacgtcctc gccatcgaac agcacgcggc cttcctcctg ctgctcaagg ccggcgatga
     6961 tacgcagcag cgtcgtcttg ccagagccgg aggggcccag cagggcgatg aattcacccg
     7021 ggcggatcgc caggtcaacg ccatgcagcg ccggataatc accaaagcgc ttggcgatat
     7081 ggttcagctc gatcaatgcc tcgctccctt gttgccgagg tcgaagcgcc attccagcgc
     7141 ggtcttcaac gccagcgtga cgagcgccag gcccgccagc agcgaggcga ccgcgaaggc
     7201 gccgacaaaa tcatattcat tgtagagaat ctcgacatgc agcggcatcg tgttggtctc
     7261 gccgcggata tggcccgaga cgaccgacac cgcgccaaat tcccccatcg cccgcgcatt
     7321 gcacagcagc acgccataaa gcagccccca gcggatgttg ggcagggtga catgccagaa
     7381 ggtctgccag ccattggcgc ccagcgagag agcggcttcc tcatcgctgc ggccctgctc
     7441 ctgcatcagc gggatcagct cacgcgcgac aaaggggaag gtgatgaaaa cggtggccag
     7501 cacgatgccc ggcaccgcga agatgacctt gatgtcatgg tcgatgaacc attcgccaaa
     7561 caccccctgc gccccgaaga tcagcacgaa gatcaggccg gaaaccaccg gagacaccga
     7621 aaagggcagg tctatcagtg tgatgagcag gctttttccg gtaaattcga acttggcgat
     7681 ggcccagctt gccgccagcc cgaacagcgt gttgagcggc accgaaatcg ccgcgacgat
     7741 cagcgtgagg cggatcgccg ccagcgcatc aggggtgacg atggccgcgc ggaaggccgc
     7801 ccagccatcg cgcagcgcct cggcgaaaac ggcggccagc ggcaggccca gcagcagcac
     7861 cagccaggcc agcgccaggc cgatcagcag ccagcggatg aagggcgcct cggtggtagc
     7921 gcgtgatgcg gcggcgctca tgcctcgtgc ctccggctat aggcctgcag caggttgatc
     7981 agcagcagca gggcaaagga ggcagccatc aggatcatgg cgatggccgt ggcgctggca
     8041 taggcgaatt gctccagctg ggtgacgatc agcagggggg cgatttccgt cttgccgggc
     8101 atgttgccgg aaatgaagat gaccgagcca tattcgccca ccccccgcgc aaaggccagc
     8161 gcaaagccgg tgagcagcgc cggggcaagg ctgggcagga tcacgcggac aaaggtctgc
     8221 cagcggctgg cgcccagcgt tgcggcggcc tcttccacat cgcggcccag gtctgccagc
     8281 accggctcca cctgccgcac cacgaagggc aggccaatga acaccagcgc aatggtgatg
     8341 cccagcggcg tgaaggccac cttgatgccc aggggatcga gatagcggcc cacccagcca
     8401 ttgggagtga acagcgttgt cagcgcaatg ccggccacgg ccgtgggcag ggcaaagggc
     8461 agatccaccg ccgcgccgat cagccgcttg gcggggaaac tgtagcgcac cagcacccag
     8521 gccaccagca gaccggccgc cgcattggca aggccggcca gcgccgccgt cgtcaggctg
     8581 aggcgatagg cggccagcgc gcggggcgaa aagcccactt caagaaagcg atcgaagcct
     8641 agccctgcgg tcttccagaa cagggtggac agcgggatca gcacgatcag cgtcagccag
     8701 ccgacggtga ggcccagcga aaggccgaag cccggcagca ccgatcgggc cttgcgggcg
     8761 cgggcacggc gccggcccgc gggcaaggga ggagaggtct gtgtcatctg cagtggcctg
     8821 ctcgatagga aggggggccg gaagcgctgc tgccggcccg tcagcaaagc ggatgtcagc
     8881 gcgccgaggc gatctggtcg tagatgccgc catcgtcgaa ataggtcttc tgggccttgc
     8941 gccagccgcc ataatcgcgg tccacctggc tgagctgcag ctgcgggaac tgcacgcccc
     9001 agcgcttggc caccttggga tcgcgcgggc gatagtagtt cttggcgatg atatcctgcg
     9061 cccaggggct gtagaggaag ttgagatagg cattggccac ggccgtggtg tggtgcttct
     9121 cggtgttctt cgtcagcacc gccacggtcg gctcggcgag gatcgagacg gacggcacga
     9181 cgatctcgaa cttgtcgcgg cccagcttgt tggtggcaag gaaggcctcg ttttcccagc
     9241 cgatcagcac atcgccaatg ccgcgttcca caaaggtgtt ggtggagccg cgcgcgccgc
     9301 tgtccagcac cggcacatgg gcataaagct gctgcacata gctgcgcgcc gcgccatcgc
     9361 cgccatgctt gcggccatag gtccaggcgg ccaggaagtt ccaccgcgcg cccgagctgg
     9421 tcttgggatt gggcgtgatc acctgcacgc ccggcttcac cagatcggcc cagtcgtgga
     9481 tgcccttggg gttgcccttg cgcaccagga agacgatcgt gctggtgtag ggcgccgaat
     9541 tataggccag cttcttctgc cagtcggggc tgagcagctg gcgcttgtcg gccagcacgt
     9601 cgatgtcata ggccaggccc agcgttgcca catcggcctc cagcccatcg aggatggccc
     9661 ggctctgcgc gccagagccg ccatggctgg tgcggaaggt gacatcctgg ccggtgcgct
     9721 tcttccacca ttcggcaaag gccgggttga tcgcctcata cagctcacgc gtgggatcgt
     9781 aagacacatt ggtgatctgg gcgggcggcg ggccagccag cgccggcgcg gcggcaaagc
     9841 ccgcagcggc aatcgcaagg ccggcaaggc tgccagcgag gatggaacga cgcttcatgt
     9901 cttggatctc ccttggaacg gccgaattaa ttcaatcaaa aaagttgagt ataaggcgaa
     9961 ggaaaagccg cccgatgccc aagggcccaa agacccctgt tccacatcgc gcggcttaat
    10021 ctcaattcca tagatagaca tttatccgcg gcgcaggcaa gcatcctgcg ccagggcagc
    10081 gcaaagcaat ttttgccacc tgccaagccc ggctgttcat ggaagggaaa ggtcgggaaa
    10141 aagagcgatc tgccgcagcg cacaagaaaa gggggcaaag agccgatgcc cctgccccct
    10201 tcgcttgcgg tcaggctggc ggcttcagcg cgcgcccagc caatcgatca gccctgcagg
    10261 cgctgccgcg ccagcggtga agctggtggc ctcatccacc ggcccctgcc ccttgtagac
    10321 cgccacttgc ggccaggcat agaccggccg cgcgcgcggc ggcagttgca cggcatccgg
    10381 ctccagcccg ggcggcgggc ccatttcgcc gggcttgccg ccggggccac ccgggccgaa
    10441 accctgggca tggcgcgcgc cttcgggcgg cggcggcatc tgaccatcag cgcccggagg
    10501 cgggccgaac tggccgccag cgcccgacgg cggcccccag tggcgcccct cgcccggccg
    10561 gccgccggcc atgccccgca tgccgccggg ctggccggcg cggccctggc tggcccggtg
    10621 cgcttccacc gcctgcggcg cggagccctt ttccacccac gccatcagct cttccagcag
    10681 cggcgccgcg ctcggcccat cgccgcccga gcaatggccc atgcccggca gcaggaacag
    10741 gcgcagggcg gcatcgcgct tgtcggcgcc cagcctggcg cccacccgct cgaaataatc
    10801 cagcgtggaa agcgggctga tatgcggatc cgcccagccg tgatagagga tcagcttgcc
    10861 gccatggcca gtgaacagcg acagatcagc attatcggca tcgtagaagg cgcgggccgc
    10921 ttccagcttg cgggccatct cctcggtgaa ggagaaattg gcgatggtgt aggcaggcgc
    10981 cgcatcgggc gcgaacagca ggtggttgat cgaggacagc gcgatgatgc cggaaaacac
    11041 cggctgatca gcactgcgcg gcacaaaaac gcccacccag ttcagctcgc tgccgggcat
    11101 cagggcgccc gcttccagcg ccttgccatc ggcggtgtgg gcgccggcat agaccttgcg
    11161 caccgcggcc acctgctgcg cggtgaggca ttggcccgcc tgataggcgc cggcacattg
    11221 caccacggcc ggatcgaaat ggcaggcgcg cggatcggtg atctgcccgt cctccagccc
    11281 gtcgagcccg tcgcaggcct tcagcacggc ggcatgcagc ggcgccatat cggccgccag
    11341 caggatcggc ttgccatcgg cgccggtgtt ggccgcggcc agccaggcgt ggtggaagct
    11401 gttctgatag gcaaaatgca gcgccggggc gccggccaga atgccatcga aatcgccggg
    11461 gaagcgctgc gcctcgatca gcgcctcgcg cccgccatcg ctgcagccgg caaaataggc
    11521 atagcgcacc gatttgccat agaaggcgcg gatcagcgcc ttggccgcca cggccgtttc
    11581 atgcagcgag cgatcggcga aatcctgccg ctgggccaat gacttgcccc aggcggcatc
    11641 catgccctga tggcccatat cggtgctggc aatggccacc gagccatccg ttaccggcgc
    11701 gcagctttcc gcctggggcg catcgatgcg cagcatgccg cacaggccgc cgcagccggt
    11761 ttgcagatag cgctgcgtcc agcccttcag cggcaggcgc agttcgaaat gcacttgcgg
    11821 gaagatcgtg cccgacacct tgcaataacc ctgcgcgctg tcggtgccgg ccacttcctc
    11881 ggcggcaagg gtggttttgg ccatcacctg ccggctgatg tcgagcgcgg ccaggctggc
    11941 gcagcccacc gccggggcca gcggcgccag atcggcgatc ggatgcaccg gcaccgcgcg
    12001 cggcgcatgg ggggcgggcg cggccatcgg cggcaggctg atgccgcgct gctcgccgcc
    12061 agcatcgtcg ggcccgccgg gctgggccaa ggccgcgccg gccggcagca gcagggctgc
    12121 cagcagggcc agcgcaaggc tgagaattcg tcgtatcatg cgtgtctcca ggacagaaag
    12181 ctgcgcgagg gccgtcgcgc gccctctccc ccaaggccgc ctgtttgcca gcgccgggca
    12241 gcgaagcaca gtaacgaatt gtcgcaaacg gggcgttcag gccgccagcc agtccttcag
    12301 gaaccacagc cccagccccg gccgcgccag cgcccgttcc agcgcatagg gccaccagcc
    12361 cgggccaaag ggcagatagg tgcgcaaggg cacgccgcgc gcgcgcgcca cgcccgccgt
    12421 ttgccgcatg ggcaggccgc gcaattgctc cagctcgcaa ggggtagcgc cttcctgcag
    12481 caggtcgagc gcgcaggcgg ccagatcggc atcatggctg gccaccgcca caggcgccgc
    12541 ccgccccgcc agaaagcccg ccagcgccat gaaacggcgc gtgacatggc catcctcgcc
    12601 ggctggatca gcccattcgc ccttcaccaa gcggatcgcc accggcatgt cgcgcaggcg
    12661 ccgagcatcg gccaggctgc gccgccagcg cgccggcagc gccagcccca gcggcaccgg
    12721 ggcgccggtt gcagaaggcc cgcccgccag cttctccatc gccgccagcg tggcatcggc
    12781 atggtgggcg gcatgggcat cgaacaccag ccccaccccg caagcgcccg cctccgccgc
    12841 cagttcggcc agaaggccgg catcaaagcc cagcggcggc gccttcaccg ccagcagggg
    12901 cgctgcggcg ccaacgcccg ccagcgcccg caccgcggcc agattggcgc tggcaatggc
    12961 cgctggcggc atatccgccc gatggaagaa acccagcgtg acccgctgcc ccagcgccac
    13021 ccgcgccgcc gcccagcgcg ccgccaccgc cgccgtgggc gccggcaaca ggccgcccag
    13081 ccgcgccggc aggccatacc ggcacgccac cgcccaggcc ctgaggcggg ccctgccgcg
    13141 caggcttgcc ctatcgcgca tcagccgggc cctggccctg tggcgcgcgc gcgcagcttc
    13201 tgcccggccc aggcggcgcc atcggccagc aacgcggcca gcccggccgg cgcgaaggga
    13261 tagatgcgga tatcaaggca ggggttggcc gcgcgcgtcc acaaatcggc gatccagcgt
    13321 tccatgccgc ccagcagctc gaaagaggaa agccccgccc gcgccgcctc gcccaggcaa
    13381 tgcagcatca gcagattgcc cggcgagcag gcgcaatgcg cctcgtcata gccgatcttg
    13441 aacagccagt accgtccttg ccacatagct cccatctgca tggcgacagc gcgcccgtca
    13501 atccgcagaa aggcgaagcg ccccagacct tcgtcgcacg cctggcgcat ccagccgcga
    13561 aagaaggccg ccttggccgg atcgcagcgc atggcgctgc cggcggcgcg cttccagccg
    13621 ctttgctcca ccgccaccgc ctcgtcgaac agcgcatcga agcgggcagg cccagggcac
    13681 agcatctcgt agctgaccac gccgcaggcc gctgcccgcc gcgccgcccg cctgagatcg
    13741 gaacggcggc cggggttgag ctgactggcc ggatccgccc agccggcatg gagagcaatg
    13801 gccggcgtgg gcgcggcagc gcgcgccacc agccagccgc gcccgcgcaa ggcccgccgc
    13861 agcgccggca ccagcgcaga gccggccggc acccggtgca gccgcaaggc cagccgccgg
    13921 cgcgccagcc cctcggccag cgcggcggcg gcggccgcat cggcataaag cgcatcgttg
    13981 ggttcataga cctcgcaagg gccgatcatc cgccagcgcg ccagccagcc gcgctcgcgc
    14041 accagcggca acagggcacg caaccgcccg cccgcttcca ccaccagcgc gcgggcatcg
    14101 gcatcggcca gccagcagcg agccacatcc agtaggaagg ccggcccctg catcggcagg
    14161 cagcccgctt cggccagttg ccgccagccc gccagcatat ccggctgcgc ggccatatcg
    14221 gccagcggca ccgcgcgcag cacgggccgc gccggccgcg ccgccatggc gcccggcacc
    14281 agggcgccgg cgctgccttg agcctgtgac acgagtgatc cccttcaccc cccggtttaa
    14341 gggcaaatcc taaagaaatc ctgcccgcgc tggaccccac actggaccat gccgcccgcc
    14401 ccgcctaaac aggggccgca agggctcaat caggaaaaag gcacggcatg gcggcggctt
    14461 ggcaagt
//
LOCUS       DASRYE010000122         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_162902,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000122 DASRYE010000000
VERSION     DASRYE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162902"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFF98_06895"
     CDS             complement(<1..990)
                     /locus_tag="VFF98_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZU63390.1"
                     /translation="MSEQILTAQQMRTAEAELIAAGTSVEALMRLAGQGAAGFVWRMA
                     GPGRMVTVLTGAGNNGGDGWVLAEAIRQRGGRVQVVMAAEPRTPAAISARAAYGGAVV
                     AGAGASGAVLVDCLFGTGLTRPLEAGDLALLQGLAARHGACVAVDLPSGIEADSGAAL
                     NEGLPDYQLTIALGAWKFAHYLMPAAARMGGLRLVEIGVAPVAGAAQVLARPGLAAPA
                     ADAHKYRRGLVGVVLGAMPGAAVLAGRAAQGAGAGYVKLLGDGPLAVAVPADLVVAPR
                     DLAEPRLGALLVGPGLGRDGEARARLAEALGAGHRLVLDADALHLLRRGDALPE"
     gene            complement(987..1313)
                     /locus_tag="VFF98_06900"
     CDS             complement(987..1313)
                     /locus_tag="VFF98_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63391.1"
                     /translation="MLLAMGLGACHRGAPPAPPPPGTAIACALDGGDWAQECRMERLA
                     TGAGGASLVVLHRPDGGFHRLRLGRDGTLALADGAGTLALDSPTDLRVGADLFRLPLE
                     RLKLAP"
     gene            complement(1352..>3104)
                     /gene="ilvD"
                     /locus_tag="VFF98_06905"
     CDS             complement(1352..>3104)
                     /gene="ilvD"
                     /locus_tag="VFF98_06905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZU63392.1"
                     /translation="DFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEF
                     NTIAIDDGIAMGHDGMLYSLPSRDLIADSVEYMVNGHCADAMVCISNCDKITPGMLMA
                     AMRLNIPVVFVSGGPMEAGKVVLRGKEHALDLVDAMVAAADEAFTDEEVQTIERSACP
                     TCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADRQGLFERAGRLIVELCKRYYE
                     GEDASVLPRAIASFEAFENAMSLDIAMGGSTNTVLHLLAAAQEAGVDFTMNDIDRLSR
                     RVPCLSKVAPAKNDVHMEDVHRAGGIYAILGELERAGLLHADLPTVHSPSLREALAKW
                     DIGRSNDAQVQHFYRAAPGGVPTQVAFSQNRRWESLDTDREKGVIRSAEHAFSRDGGL
                     AVLFGNIAREGCIVKTAGVDESILKFTGPAKVYESQDAAVTAILTGQVEAGDVVVIRY
                     EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHASPEAAEGGTI
                     GLVETGDVIEIDIPGRTINLAVADAVLDARRAVQEAKGWLPAAPRKRVVSTALKAYAA
                     MTTSAARGAVRDLSQLK"
BASE COUNT          493 a         1176 c          990 g          445 t
ORIGIN      
        1 ttccggcagc gcatcgccac gccgcagcag atgcagcgca tcggcatcga gcaccagccg
       61 gtggcccgcg cccagcgctt ctgccagccg cgcgcgggcc tcgccgtcgc ggcccagccc
      121 cggccccacc agcagcgcgc ccagccgggg ctctgccaga tcgcgcgggg ccaccaccag
      181 atcggccggc accgccaccg ccaaaggccc gtcgcccagc aatttcacat aacccgcgcc
      241 cgcgccctgc gccgcccggc ccgccagcac cgccgccccc ggcatcgccc ccagaaccac
      301 gcccacaagg ccgcggcggt atttgtgggc atcggccgca ggcgctgcca ggcccggccg
      361 cgccagcacc tgcgcagcgc ccgccaccgg cgccacgcca atttccacca gccgcaggcc
      421 gcccatgcgg gccgccgccg gcatcaggta atgggcaaat ttccaggcgc ccagcgcgat
      481 ggtcagctga taatcgggca ggccctcgtt cagcgcggcg ccgctatcgg cctctatgcc
      541 gctgggcaga tccaccgcca cgcaggcccc atggcgcgcc gccagcccct gcaacagcgc
      601 cagatcaccc gcctccagcg ggcgcgtgag gccagtgccg aacaggcaat cgaccagcac
      661 tgcgccactg gccccggcgc ctgccaccac cgccccgcca taggccgcgc gggcgctgat
      721 ggccgccggc gtacgcggct ctgcggccat caccacctgc acacggccac cgcgctggcg
      781 gatcgcctcg gccagcaccc agccatcgcc gccattgttg cccgcgcctg tcagcaccgt
      841 caccatccgg cccgggcctg ccatgcgcca gacaaaaccc gccgccccct gccccgccag
      901 ccgcatcagc gcttccaccg aagtgccggc ggcaatcagc tctgcctcgg cggtgcgcat
      961 ctgctgggcg gtgaggatct gctcgctcat ggcgccagct taagcctttc cagcggcagg
     1021 cgaaagagat ctgcgcccac ccgcagatcg gtcgggctgt ccagcgccag ggtgccggcg
     1081 ccatcggcca gcgccagcgt gccatcgcgg cccagccgca gccggtggaa gccgccatcg
     1141 ggccggtgca gcacaacaag gctggcgcca ccggcacctg tggccagccg ctccatccgg
     1201 cattcctgcg cccaatcgcc gccatccagc gcgcaggcaa tcgccgtgcc cggtggcggc
     1261 ggggcgggcg gcgcgcccct gtggcaggcg cccagcccca tggccagcag cacagccccc
     1321 caccgggcct tccggctcgg gggaacgcgc atcacttcag ctgcgacaga tcgcgcacgg
     1381 cgccgcgcgc ggcgctggtg gtcatcgccg cataggcctt gagcgcggtg gacaccacgc
     1441 gcttgcgggg ggcggcgggc agccagccct tcgcttcctg cacggcgcgg cgcgcatcca
     1501 gcacggcatc ggccacggcc aggttgatgg tgcggccggg gatgtcgatc tcgatcacat
     1561 caccggtttc aaccaggcca atggtgccgc cctcggccgc ctcggggctg gcatggccga
     1621 tcgacaggcc cgaggtgccg cccgaaaagc gcccgtcggt gatcagcgcg caggccttgc
     1681 ccagcccctt cgacttcaga tagctggtgg gatagagcat ttcctgcatg cccggcccgc
     1741 ccttgggccc ttcatagcgg atgacgacga catcgcccgc ttccacctgc ccggtgagga
     1801 tcgccgtgac ggccgcatcc tggctttcat aaaccttggc cgggccggtg aacttgagga
     1861 tgctctcatc cacgccggcg gtcttcacga tgcagccctc gcgagcgatg ttgccaaaca
     1921 gcacggcaag gccgccatcc ctggaaaagg catgttcggc cgagcggatg acgcccttct
     1981 cgcggtcggt atcaaggctt tcccagcggc ggttctggct gaaggccacc tgcgtgggca
     2041 caccgccggg cgcggcacga tagaaatgct gcacctgggc atcattgctg cggccgatat
     2101 cccacttggc cagcgcctcg cgcaggctgg ggctgtgcac ggtcggcagg tcggcgtgga
     2161 gcaggccagc gcgctccagc tcgcccagga tggcatagat gccgccggcg cggtgcacgt
     2221 cttccatatg cacatcgttc ttggccggcg ccaccttgga caggcagggc acccggcgcg
     2281 acagccggtc gatgtcgttc atggtgaaat caacgccggc ttcctgcgcg gcggccagca
     2341 ggtgcagcac ggtgttggtg gagccgccca tggcgatatc gaggctcatg gcgttctcaa
     2401 acgcctcgaa gctggcgatg gcgcgcggca gcacgcttgc atcctcgccc tcgtaatagc
     2461 gcttgcacag ctccacgatc agccggccgg cacgctcgaa cagaccctgc cggtcggcat
     2521 gggtggcaag ggtggaacca ttgcccggca gcgacaggcc cagcgcctcg gtcaggcagt
     2581 tcatcgaatt ggcggtgaac atgcccgagc aggaaccgca ggtggggcag gccgaacgct
     2641 cgatcgtctg cacttcctcg tcggtgaagg cctcgtcggc ggcggccacc atggcatcca
     2701 ccagatccag cgcgtgttcc ttgccgcgca gcaccacctt gcccgcttcc atcggcccgc
     2761 ccgagacgaa caccaccgga atgttcagcc gcatcgccgc catcagcatg cccggcgtga
     2821 tcttgtcgca attagagatg cacaccatgg catcggcgca gtggccgttg accatatatt
     2881 ccacgctgtc ggcgatgagg tcgcggctgg gcaggctgta gagcatgcca tcgtggccca
     2941 tggcgatgcc atcgtcgatg gcgatggtgt tgaattcctt ggcaacgccg ccagccgcct
     3001 cgatctcgcg ggcaaccagc tggcccagat ccttcagatg cacatggccc ggcacgaatt
     3061 gcgtgaagct gttcaccacg gcgatgatcg gcttgccgaa atcg
//
LOCUS       DASRYE010000123         6119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_218011,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000123 DASRYE010000000
VERSION     DASRYE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218011"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFF98_06910"
     CDS             complement(<1..335)
                     /locus_tag="VFF98_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZU63393.1"
                     /translation="MIQDQKPFASAARPAGLSPRARSAAAAYGGDAADRIRRFVPMVR
                     RLAWHLHASGREGMEVEDLMQAGMVALTECAQRHNGPGEDGFAAYAKMRVRGAMVDLI
                     RRQVPGSRGA"
     gene            complement(332..2470)
                     /locus_tag="VFF98_06915"
     CDS             complement(332..2470)
                     /locus_tag="VFF98_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZU63394.1"
                     /translation="MDRIFGAGGPSALALPGGILTIIVLMVVPIPAFMLDVFFVFNIA
                     LSVAVLMASMNAAKPLDFSSFPSVLLFATLLRLALNVASTRVVLVHGHEGGAAAGHVI
                     ESFGEFLIGGNFAVGLFVFMILMIINLVVITKGAGRVSEVSARFTLDALPGKQMAIDA
                     DLAAGLMTADEARTRRREVGTEADFYGSMDGASKFVKGDAVAALLILGVNIIAGFCLG
                     MISHGLTAADAAQKYITLAVGDALVAQVPALLLSIAAAVIVTRVSDSRDLAGQIGGQF
                     ADPRTWLPVAMILLAIGMIPAMPQRVFLPAAAIAFYVWRQLKGRAEAEAARAAIPPPA
                     PVADPAKITLDDVSDHTLVTVELGYGLVQLVDEKRGAPLVGRITGVRKQLSQGFGFVV
                     PQFRVRDSLDMPAHDYRVLLGGVLLGGATVLADKVLAIDAGEANARHMLKGTPTVDPS
                     FGCPALWIEPAMRDLAIAEGFLTVDASTVIATHLNQLLSERPHLLLTPDEVKALVESL
                     KSRAAGLVETVYPTPLALAALTRVLRALLEDGIPIAHPLPILASLSRALQITTEHERL
                     VDLLRIDLGAVIVGRIVGPAERLPVVTLDAALEAMIAQGMVDPATGQPLIEPDLARRI
                     ADQVVAILASRGAGAGVALIVQPRLRRALASLLRLRAPACAVLSIAELPESQPVEVIA
                     VIGAEPPAPPALPAPGGAGSSSPAFESMVA"
     gene            complement(2485..3405)
                     /locus_tag="VFF98_06920"
     CDS             complement(2485..3405)
                     /locus_tag="VFF98_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZU63395.1"
                     /translation="MSQPDLNLMSSGMAGMAGAGWAAADGAMAADSSGVRAAIARASA
                     ATGVDFSYLVAEARLESGFNPGARAAGSSARGLYQFTNGTWLQTLQRHGAALAGDGAD
                     LAAALADPAARARLMALRNDPDASALMAASLAADNQQALGSALGRAPDATELYLAHFL
                     GTDGAVKFLSALAADPGQSAASLLPKAAAANRAIFFDESGAPRSLAGMMDLLRGRLAG
                     AMADSSTLAAGSGWALATPAAAAAPVDNSLAGQFAAARADAASLAGTGTGSSMAATLQ
                     STFTAMADATGTPPSATVQAAYGRMAAMGF"
     gene            complement(3495..3836)
                     /locus_tag="VFF98_06925"
     CDS             complement(3495..3836)
                     /locus_tag="VFF98_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZU63396.1"
                     /translation="MSVPQTLRETLRQMVSVLESERQALAGLDLDGIVGCVHDKQLLC
                     GLLEENRPEDIDDECRGLLDAARRLNEVNRQLRNLIAANVAARLEAITGSPAIYRAHG
                     GPAYAYAGGRA"
     gene            complement(3833..4171)
                     /gene="flgM"
                     /locus_tag="VFF98_06930"
     CDS             complement(3833..4171)
                     /gene="flgM"
                     /locus_tag="VFF98_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZU63397.1"
                     /translation="MSSIGSGPGIGSGSQLEISALRQAAAVDARGNQATTAAAPQAAA
                     ADGGQSGAVSSAGSGMVAAASAGAGAAPIDNERVAVIKKAIQTGNYPVIPTKIGDAMI
                     AAGVMLRTGK"
     gene            complement(4291..4809)
                     /locus_tag="VFF98_06935"
     CDS             complement(4291..4809)
                     /locus_tag="VFF98_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HZU63398.1"
                     /translation="MPHRSFRHVLAALLPVILCPSAAQAAPAAFTDPAAIDREVATFT
                     GKAIGQPGGAAMPVDRRLRLAVCSSPLALSWRAQRRDTVLVECLDPGSWHVFVPVKAT
                     ETGATVVARGELVTISVAGEGFTVSQSGEALDGGALGEWIRVRGLRDGGAHSDAVRAR
                     IIGPGMVEVVLP"
     gene            complement(4876..5301)
                     /locus_tag="VFF98_06940"
     CDS             complement(4876..5301)
                     /locus_tag="VFF98_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63399.1"
                     /translation="MTTRTPIGWQTMLADLSLILFMVTAAAMAEKPGDARPAPPPPPP
                     PPPVGLADPARAEPLAIWRARAGGPGLAAWLASQPADPRQQLTITARCRPGGQKAAIA
                     RAEALLHEAGAGAAAARIIVQTGGSADLYASLAYDRAPG"
     gene            complement(5298..6044)
                     /locus_tag="VFF98_06945"
     CDS             complement(5298..6044)
                     /locus_tag="VFF98_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZU63400.1"
                     /translation="MFYSLFMDGPTLAIVLGGTALATFLRCGWADVRRAGGQLLAALL
                     PFAARSRAFDAEALRARLATQVQDIRRDGVLRARPRRIGDAEFDGALAVLEARRQVPR
                     MAEALEEARARRRALADSAARTLLQAAELAPVFGLAGTLISLSKLPVNGIDRGAYMAA
                     IGMAVHATLYGLVTANLLLAPLGRLVERRAAREDAARQQIALWLLGEIELARPHLADM
                     QEAGKAAAQRRAEPVASPSAPAAEAEANRP"
BASE COUNT         1014 a         2188 c         2104 g          813 t
ORIGIN      
        1 gccccgcgcg atccgggcac ctgccggcgg atcagatcga ccatcgcccc cctcacccgc
       61 atcttggcat aagcggcaaa accatcctcg ccggggccat tgtgacgctg ggcgcattcg
      121 gtcagcgcca ccatgccggc ctgcatcagg tcttccacct ccatgccctc gcggcccgag
      181 gcgtgcaggt gccaggccag gcgccgcacc atcggcacga aacggcggat acggtcggcc
      241 gcatcgccgc cataggccgc agcggcgctg cgcgcgcggg gcgaaaggcc ggcgggccgg
      301 gcagcgctgg cgaagggttt ctgatcctgg atcatgccac catgctttca aacgcgggag
      361 aggaagaacc agcgccaccg ggcgccggaa gagcgggagg agcgggcggt tcggcgccga
      421 tcacggcgat cacttccacc ggctggcttt cgggcagttc ggcaatggaa agcaccgcgc
      481 aagccggcgc gcgcagccgc aacaggctgg ccagcgcccg gcgcagccgc ggctggacga
      541 tcagcgccac cccggcgccc gccccccggc tggccagaat ggccaccacc tgatcggcga
      601 tgcggcgggc cagatccggc tcgatcagcg gctggccggt ggccggatcg accatccctt
      661 gcgcgatcat cgcttccagc gcggcatcca gcgtgaccac cggcagccgc tcggccgggc
      721 cgacaatccg cccgacgatc accgcgccca gatcgatgcg caggagatcc accagccgct
      781 cgtgctcggt agtgatctgc agcgcgcggc tcagcgaggc gaggatcggc agcgggtggg
      841 caataggaat gccatcttcc agcagcgcgc gcagcacccg ggtcagcgcg gccagcgcca
      901 gcggcgtggg gtagaccgtc tcgaccaggc ccgcggcccg gctcttcagg ctttccacca
      961 gcgccttcac ctcgtcaggc gtcagcagca gatgcggccg ttccgacagc agctggttca
     1021 gatgggtggc gatcaccgtg ctggcatcca ccgtcaggaa gccctcggcg atggccagat
     1081 cgcgcatcgc cggctcgatc cacagcgccg ggcagccaaa gctggggtcc accgtggggg
     1141 tgcccttcag catgtggcgg gcattggcct cgccggcatc gatcgccagc accttgtcgg
     1201 ccagcaccgt ggcgccgccc agcagcacgc cgcccagcag cacgcgataa tcatgcgcgg
     1261 gcatgtcgag cgaatcgcgc acgcgaaact gcggcacgac aaagccaaag ccctgcgaca
     1321 gctgcttgcg cacgccggtg atgcggccca ccagcggggc gccgcgcttc tcgtccacca
     1381 actgcaccag cccatagccc agctccaccg tcaccagcgt gtggtcggac acgtcatcga
     1441 gggtgatctt cgccggatcg gccacgggcg cggggggagg gatggcggcg cgggccgcct
     1501 cggcctcggc ccggcccttc agctggcgcc acacatagaa ggcaatggcc gcggccggca
     1561 ggaacacgcg ctgcggcatc gccgggatca tgccgatggc caacaggatc atcgccaccg
     1621 gcagccaggt gcgcggatcg gcaaattgcc cgccgatctg cccggcaagg tcgcggctgt
     1681 cggacacgcg ggtgacgatg accgccgccg cgatcgacag cagcagcgcg ggcacctgcg
     1741 ccaccagcgc atcgcccacg gcaagggtaa tgtatttctg cgcggcatcg gcagcggtga
     1801 gcccgtggga aatcatgcca aggcagaagc cggcaatgat gttcaccccc aggatcagca
     1861 gcgctgccac cgcatcgccc ttcacgaatt tgctggcgcc atccatcgag ccatagaaat
     1921 cggcctcggt gcccacttcg cggcggcggg tgcgggcctc gtcggcggtc atcagcccgg
     1981 cggccagatc ggcatcgatc gccatctgct tgcccggcag cgcatccagc gtgaaacgcg
     2041 ccgacacttc cgacacgcgc cccgcgccct tggtgatcac caccagattg atgatcatca
     2101 ggatcatgaa gacgaacagg ccgacggcga aattgccgcc gatgagaaat tcaccaaagc
     2161 tttcgatcac atgaccggcc gccgcacccc cttcatggcc atgcaccagc accacgcggg
     2221 tggacgccac gttgagcgcc agccgcagca gggtggcaaa cagcagcacc gaggggaagg
     2281 aggagaaatc cagcggcttg gcggcattca tcgaggccat cagcaccgcc accgacaggg
     2341 cgatgttgaa gacgaagaac acatccagca tgaaggccgg aatgggcacc accatcagca
     2401 cgatgatggt gagaatgccc cccggcaagg ccagcgcgga agggccgccg gcgccaaata
     2461 tcctgtccat gcccgcgcct cccctcaaaa gcccatcgcg gccatgcggc cataggcggc
     2521 ctgcacggtg gcagagggcg gtgtgccggt ggcatcagcc atggcggtga aggtgctttg
     2581 cagcgtggcg gccatggagc tgccggtgcc ggtgccggca aggctggcgg catcggcccg
     2641 ggcggcggca aactggccgg ccagcgaatt gtccaccggg gcggcagcgg cggcgggtgt
     2701 cgccagcgcc cagccagagc ccgcggccag ggtggaactg tcggccatgg cgccggccag
     2761 gcggccccgc agcaggtcca tcatgcccgc cagcgagcgg ggggcgccgc tctcgtcgaa
     2821 gaagatcgcc cggttggcgg cggccgcctt gggcagcagg ctggccgcgc tctgccccgg
     2881 atcggcggcc agcgcggaaa ggaatttcac cgcgccatcg gtgccgagga aatgggcgag
     2941 atatagttcg gtagcatcgg gcgcgcggcc cagggcgctg cccagcgcct gctggttgtc
     3001 ggcggcaagg ctggcagcca tcagggccga ggcatcggga tcgttgcgca gggccatcag
     3061 ccgggcgcgg gcggcgggat cggccagcgc cgcggccaga tccgcgccat cgccggccag
     3121 cgccgcgcca tggcgctgca acgtttgcag ccaggtgcca ttggtgaact gatagagccc
     3181 gcgggcgctg gagccggcag cccgggcacc ggggttgaag cccgattcca gccgcgcctc
     3241 ggccacaaga taggaaaaat cgacgccggt ggcagcgctg gcgcgggcga tggcggcgcg
     3301 cacgccgctg ctgtcggccg ccatggcgcc atcggcggcg gcccagccgg cgccggccat
     3361 gccagccatg ccgctgctca tcaggttcag atcaggctgg ctcaagccac gctcccgtca
     3421 aaatggttcg ggagggatga agcaaacccc atgccaaatg ccccgcacgc cgtggcgcgg
     3481 ggcctggccg tttctcaggc gcggccgccc gcataggcgt aggccggccc gccatgggcg
     3541 cgatagatcg ccgggctgcc ggtaatcgct tccagccggg cggccacatt ggcggcaatc
     3601 aggttcctga gctggcggtt aacctcgttc aggcgccgcg cggcatccag caggccgcgg
     3661 cattcatcgt cgatatcctc gggccggttt tcttccagca ggccgcacag caattgcttg
     3721 tcgtgcacgc aaccgacgat gccgtcgagg tccagcccgg caagggcttg ccgttcgctc
     3781 tcgagaacgg aaaccatttg ccgcagggtc tcgcgcaggg tctgtggaac gctcatttgc
     3841 ctgtcctcag catcacgccg gcggcaatca tcgcgtcgcc gatcttggtg ggaatgaccg
     3901 ggtagttgcc ggtctggatg gctttcttga tgacggccac gcgctcgttg tcgatgggcg
     3961 ccgcgcccgc gccggcactg gccgcggcca ccatgccgct gccagccgag ctgaccgcgc
     4021 cagattgccc gccatcggcc gcagccgcct gcggggcagc cgccgtggtg gcctggttgc
     4081 cgcgcgcatc caccgcggcc gcttgccgca gggcagaaat ttccaattga gaccccgatc
     4141 cgatgccagg gccggatccg atgctggaca tggtgccgct ccttgctttt acaagccgaa
     4201 gaacggcgcc ccgcaagagc gtttaagtgc agcggaagga ttttttaacg ggccagcttt
     4261 ttcaggccgg ccttgtcagg ccaggccctt tcagggaagc accacttcca ccatgcccgg
     4321 gccgatgatc ctggcgcgca cggcatcgga atgggcgccg ccatcgcgca ggccccgcac
     4381 gcggatccat tcgcccagcg cgccgccatc cagcgcctcg cccgattgcg agacggtgaa
     4441 accctcgccc gccacgctga tcgtcaccag ttcgccgcgc gccaccacag tggcccccgt
     4501 ttccgtggct ttcaccggca cgaaaacatg ccagctgccc ggatccaggc attccaccag
     4561 caccgtgtcg cgccgctgtg cccgccacga cagcgccagc ggcgaggaac agaccgccag
     4621 ccgcagccgc cggtccaccg gcatggcagc gccgcccggc tggccaatcg ccttgccggt
     4681 gaaagtggcc acctcgcggt caatcgcggc cggatcggtg aaagcggcgg gcgctgcctg
     4741 agccgccgag gggcacagga tcacgggcag cagggcggca agcacatggc ggaacgagcg
     4801 atggggcacg ggcgaaacct ttacagcggc gaattgccgc ccctgtgcta aacaagtttc
     4861 gtgccagatg ccgcttcagc ccggcgcccg gtcataggcc agcgaggcgt agagatcggc
     4921 cgacccgccc gtctggacga tgatgcgggc agccgcagca ccggcgcccg cctcgtgcag
     4981 cagcgcctcg gcccgggcga tggcggcttt ctgcccaccc gggcggcagc gcgcggtgat
     5041 cgtcaactgc tggcgcggat cggccggctg gctggccagc caggcggcaa ggcccggccc
     5101 acccgcccgc gcccgccaga tcgccagcgg ctcggcccgg gcaggatcgg ccaggcccac
     5161 cggcggcggc ggaggaggcg gcggcggcgc ggggcgggca tcgcccggct tttccgccat
     5221 ggccgcggcc gtgaccatga acaggatcag cgacagatcg gccagcatcg tctgccagcc
     5281 gatgggcgtg cgggtggtca tggccggttc gcctctgcct cggccgccgg agcagacggg
     5341 gaagcaaccg gctcggcgcg gcgctgggcg gcggccttgc ctgcctcctg catgtcggcc
     5401 agatgggggc gggccagctc gatctcgccc aaaagccaca gggcgatctg ctggcgggcg
     5461 gcatcctcgc gggcggcccg ccgctccacc agccggccca gcggcgccag cagcagattg
     5521 gccgtgacca ggccatagag cgtggcatgc accgccatgc cgatggccgc catataagcg
     5581 ccccggtcga tcccgttcac gggcagtttg cttagcgata tcaaagtgcc ggccaggccg
     5641 aacaccggcg ccagctcggc cgcctgcagc aacgtgcggg cggcgctgtc ggccagcgcg
     5701 cggcggcgcg cgcgtgcctc ttccagcgct tcggccatgc gcggcacctg gcggcgcgct
     5761 tccagcacgg ccagcgcgcc atcgaattcg gcatcgccaa tccggcgcgg gcgggcgcgc
     5821 agcacgccat cgcggcggat atcctgcacc tgggtggcca gccgcgcgcg cagggcctcg
     5881 gcatcgaacg cccggctgcg cgccgcaaac ggcagcagcg cggccagcaa ctggccgcct
     5941 gcccgccgca catcggccca cccgcaacgc aggaatgtgg ccagcgccgt gccgcccagc
     6001 acgatagcca gggtcggccc gtccatgaac agggaataga acattgcgcg cagctccttc
     6061 ccacggttgg acggatggcg ccggccgaat ttcaggggcg gcgcaacttt gccggcggg
//
LOCUS       DASRYE010000124        13263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_162907,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000124 DASRYE010000000
VERSION     DASRYE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162907"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFF98_06950"
     CDS             <1..268
                     /locus_tag="VFF98_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63401.1"
                     /translation="RREEEPAGEPVAEAAAPAPAPEQPAAVALDPTSLPPAISTGRRS
                     SKAASAAAAAAAAAATGEEPAPAPKRRTRRKAVSDDGEALQTAS"
     gene            392..2014
                     /gene="lnt"
                     /locus_tag="VFF98_06955"
     CDS             392..2014
                     /gene="lnt"
                     /locus_tag="VFF98_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZU63402.1"
                     /translation="MADPRSARLRLLLLVRLLWGVAWRVGLIAALGALAACAFRPLAW
                     WPAMLVGVGALAVLAGRVPRARWALLVGWLWGLGHFTLGLNWIATAFTYQAKMPAWLG
                     GLAVLLLSAYLAVFPALAAWGAWAARRRPLAQALAFAGCWALAEALRGWLFTGFPWNP
                     LAASLLGDFAHPGLAALLPWLGTYALSALAALLAAVVGQAVLALWQGERSPWRLAPAG
                     AALALLALAFHGPAKAPQEGGHVAYTVVQPNIPQPELNDPTYFEDQFQKTARLSRAIG
                     PAGPRLVLWPESGVPDFLREGYPLEYYQATTFAADPLLARLRIGRLIGPQAQLLTGSI
                     DLIMAGNRVAGARNVVTGLDSAGRITGSYAKAHLVPYGEYLPMRWLLKPLGLERLVPG
                     DMDFTPGPGPATLDLGVLGKAGVQICYEIIFPGAVVDGAHRPEYIFNPSNDGWFGDWG
                     PPQHLAQARLRAIEEGLPVLRATTNGISAVVDSHGVVVQSAGRDVAARLDGTVPPALA
                     PTPFASAGNALSLGWALLLLVAAALVLRRRER"
     gene            2054..3271
                     /gene="metK"
                     /locus_tag="VFF98_06960"
     CDS             2054..3271
                     /gene="metK"
                     /locus_tag="VFF98_06960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZU63403.1"
                     /translation="MRSEFLFTSESVSEGHPDKVADQISDAIVDLFLAKDPEARIACE
                     TLTTTQLVVLAGEIRGRGIMDEAGNWAPGVPEEVEAAVRATVKRIGYEQEGFHWESLR
                     FENNLHPQSAHIAQGVDAGANKDEGAGDQGIMFGFACDETPELMPATLYYSHKILERL
                     AADRHAGTAPFLEPDAKSQVTLRYEQGKPVAATAIVVSTQHKAGYDEGAGQAELNAYV
                     KQVVGEILPAGFLSADTAWHINPTGSFEIGGPDGDAGLTGRKIIVDTYGGAAPHGGGA
                     FSGKDPTKVDRSAAYVARYLAKNVVAAGLASRVTIQLSYAIGVARPLSLYVDTHGTGK
                     AEDAALEAAIARVAEEKLGGLTPRGIRLGLGLNQPIYSPSAAYGHFGRTAEGAYFPWE
                     RTDLAEALVAALA"
     gene            3424..4209
                     /locus_tag="VFF98_06965"
     CDS             3424..4209
                     /locus_tag="VFF98_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZU63404.1"
                     /translation="MRSILVFADHADPATETRLETALSLARSSGGHVSVLVDTPIARF
                     MAMDAMGGSYLAADAIRDAVERDDAYAEALAERLKREDVPFDIQRGEDEPVDALADAS
                     RLADVIVLSRGCLFAGQLAVSARAPILVVPETGVVGVPFTRAAVAWDGSQEAAAAMKA
                     ALPLLAAAGQADVLIVSAEAAGYPAVDAVQYLSRHGVHAELHDLARAGSTEGTLAAEV
                     HKIGADLLVMGAYGHSRFREFIVGGVSRAFLESEGGPTLLLAH"
     gene            complement(5176..6276)
                     /locus_tag="VFF98_06970"
     CDS             complement(5176..6276)
                     /locus_tag="VFF98_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZU63405.1"
                     /translation="MPFPLFAASGPVPLPTPTLDTDALPSFSEFLHWSHRLTHSAIVA
                     GVAVAIALVLHKLVFAMIAHMVRRGEGNEPTPAARRLHRSTRWLLVGVALSIADDGDW
                     LVAHLWGAVARFVMPALTGWVVYALVSVGAEVLAARADRVDDELAIRSRRTRIALLAR
                     SAGFVIIFITLSLILFGIPAVRHLGATLMASAGLLGLAVGAAAQPMLKSLIAGLQIAL
                     TEPIRLGDYVVVDGESGRVEDIHLSYVVVQTSDERRLIVPPTKFLDATFQNWSRVGGI
                     AGPVVIPVRPGTDIAPIRAAFLAMLQSMPEWDGRVGSLYIADVRPGVVELRLLVSAAQ
                     PTLLGALRPAVREGMMSWLQANAPDALCQEAE"
     gene            complement(6309..9158)
                     /gene="polA"
                     /locus_tag="VFF98_06975"
     CDS             complement(6309..9158)
                     /gene="polA"
                     /locus_tag="VFF98_06975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZU63406.1"
                     /translation="MDKPPHLYLVDGSAYIFRAYHRLPPLTNPEGVPVGAVYGYTTML
                     WKLAEDLHKADGPTHLAVILDKAGRSFRNDIYPAYKANRPPAPEDLVPQFPLIRDATR
                     AFSLPCIEEEGLEADDLIASYARAAQARGWSVTIVSSDKDLMQLVGDGPDGSRIDMLD
                     TMKNQRIFLPEVAEKFGVVPGQVGDVLALMGDAVDNVPGIRGIGPKTATKLIQEHGDL
                     ESALAAAPAMKASKLRENLIEQAEMARLSRVLVALKEDCALPLPLEAFALGQIPEAPL
                     AAFLHTHGFTSLAKRLDAGRGSPERATQLHPAKAEHAAAAPAPAGTRQPLADLPPIDH
                     ARYECVQSAEALARWVARGLAARLIAFDTETSALDAIGADLVGISLATGPGEACYIPL
                     GHRGTDMFAETPRQIAKAEAVALLKPLLENPAVLKVGQNAKYDLNVLAREGIAVGPID
                     DTMVLSFDLDAGRGEEGPGGISFGGHGMDELARRHLGHSCIAFKDVCGTGKKQISFAE
                     VPLDRATAYAAEDAEVTWRLHRLLKPRLAGEAATRIYERVDRPLIPVVAGMERRGIRV
                     DRERLAGLSAQFAEAIAGLEGEIHALAGTPFTIGSPKQLGEILFDQMGLKGGKKGKSG
                     QYSTDQSVLEKLEGDGVAIAGKVLEWRQLAKLRSTYTEALQAAISPATGRVHTSYSLV
                     GAQTGRLSSTEPNLQNIPIRTEIGRQIRDCFVADAGHVLLSADYSQIELRLAAHMADV
                     PALKAAFAAGADIHAATAQELFGEVTRETRGRAKTINFAILYGISRWGLAPRLGVPPE
                     EAQALIDRYFERFPGIQRYIHETLSSVREKGYSQTLFGRKCWFPRISAKNQAERQGSE
                     RAAINAPIQGTCADIIKRAMARMEAALAAAGLGHVQMLLQVHDELVFELPEADVPAAS
                     TVIRQVMAEAALPSSPLSVPLAVEIGTGPSWGAAH"
     gene            9502..9711
                     /locus_tag="VFF98_06980"
     CDS             9502..9711
                     /locus_tag="VFF98_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63407.1"
                     /translation="MRKIILAAAVAGAALSLAACKGGEAASSADTAVSADSAVSADSA
                     VSDVATGVDSAASAAASAAASATSK"
     gene            complement(9881..10909)
                     /locus_tag="VFF98_06985"
     CDS             complement(9881..10909)
                     /locus_tag="VFF98_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZU63408.1"
                     /translation="MKHTKQHLLFAAFMACALIAPPALATDDLEQAFDKVFGAQPAAR
                     PAPAPAAPVAALPVGPASSFEARLAGLANAAHGRIGVAALDLSTGSGVSVMGDQPFPM
                     ASTVKVAIVATFLEGVDQGRFRLTDRFPLMIPVPSRKFSSSVAPVHAGQALSALELIE
                     LAITRSDNRATDALLAAIGGPQAVDRWLHRAKIGGIHVNRDIATLVRDDGGVDPATTI
                     DLRDSATPQAMATLLAGLYQGQWLSMASREVLLGAMSRCLTGVHRMKALVPDEAIIGH
                     KTGTLSNTSSDVGFIRTADGHTVALAIYVTGQGSRPGRESRIATIARAVYDNFSDPAQ
                     GGSRVSLN"
     gene            complement(11125..12762)
                     /gene="groL"
                     /locus_tag="VFF98_06990"
     CDS             complement(11125..12762)
                     /gene="groL"
                     /locus_tag="VFF98_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049566.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZU63409.1"
                     /translation="MAAKDVKFGRDARERILKGVDTLADAVKVTLGPKGRNVVIEKSF
                     GAPRITKDGVSVAKEIELKDRFENLGAQMLREVASKANDKAGDGTTTATVLAQAIVRE
                     GLKSVAAGINPMDLKRGIDIAVAAVVENLKARSKPVADSAQIAQVGIISANGDREVGE
                     KIAEAMEKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFVTNPEKMTVELESP
                     YILIHEKKLSSLQTLLPILEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAV
                     KAPGFGDRRKAMLGDIATLTAGEMISEDLGIKLESVTLGMLGQAKKVTIDKDNTTIVD
                     GLGSAEEIKARVEQIRAQIEITTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKER
                     KDRVDDALHATRAAVEEGIVPGGGTALLYATKALAGLAGANDDQTRGIDIIRKAVTAP
                     IKQISENAGHDGAVVAGKLLDQADEALGFNAATDVYENLLAAGVIDPTKVVRTALQDA
                     ASVSGLLITTEAGIVEKPEDKPAMPAMPGGGMGGMDF"
     gene            complement(12817..13104)
                     /gene="groES"
                     /locus_tag="VFF98_06995"
     CDS             complement(12817..13104)
                     /gene="groES"
                     /locus_tag="VFF98_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZU63410.1"
                     /translation="MSFRPLHDRVLVRRVEAEEKTAGGIIIPDSAKEKPAEGVIVSVG
                     TGARAENGTVTPLEVKAGDRVLFGKWSGTEVKVGGEDLLIMKESDILGIIG"
BASE COUNT         2038 a         4320 c         4585 g         2320 t
ORIGIN      
        1 gcgccgcgag gaagagccgg cgggcgagcc ggtggccgaa gctgccgcac cggcccccgc
       61 gcccgagcag cctgctgccg tggcgcttga tccgacctcg ctgccgccgg ccatttccac
      121 gggccgccgc agcagcaagg cggccagcgc cgctgctgcc gccgccgcag ccgccgccac
      181 gggcgaggag ccggcgcctg cgcccaagcg ccgcacccgc cgcaaggcgg tgagcgacga
      241 cggcgaggcc ttgcagacgg ccagctgatc gggcgcgggc tttcgcccgc cccttcatgc
      301 atttgcggcg ggcagggctg gcaggccttg cccgccgctg tgcatgcaag aagcgcttgc
      361 acgttgatct ggcgcggcta agcgctgggg catggctgat ccccgctccg ctcgcctgcg
      421 cctcctcttg cttgtccgct tgctgtgggg tgtggcctgg cgcgtcgggc tgattgccgc
      481 gctgggcgcg ctggcggcct gtgctttccg gccgctggcc tggtggccgg cgatgctggt
      541 gggcgtgggc gcgctggcgg tgctggccgg gcgcgtgccc agggcgcgct gggcgctgct
      601 ggtgggctgg ctgtgggggc tgggccattt cacgctgggg ctgaactgga tcgccacggc
      661 cttcacctat caggccaaga tgccggcctg gctgggcggt ttggcggtgt tgctgctgtc
      721 ggcctatctg gcggtgtttc cggctttggc ggcgtggggc gcgtgggcgg cgcggcggcg
      781 gccactggcg caggcgctgg cgtttgccgg ctgctgggcg ctggccgagg ccctgcgcgg
      841 ctggctgttc accggctttc cgtggaaccc gctggccgcc agcctgctgg gtgattttgc
      901 ccaccccggc cttgccgcct tgctgccctg gctgggcacc tatgcgctgt cggcgctggc
      961 ggcattgctg gcggcggtgg tggggcaggc cgttctggcg ctgtggcagg gcgagcggag
     1021 cccttggcga ttggcgccgg cgggcgcggc gctggcgctg ctggcgctgg cctttcacgg
     1081 gccggccaaa gcgccgcagg aaggcggcca tgtggcctac accgtggtgc agccgaacat
     1141 tccccagccc gaactgaacg atcccaccta tttcgaggac cagttccaga agaccgctcg
     1201 cctgtcgcgc gctatcgggc cggccgggcc ccggctggtg ctgtggcccg aatcgggcgt
     1261 gcccgatttc ctgcgcgagg gctatccgct ggaatattac caggccacca cctttgccgc
     1321 cgatccgctg ctggcgcggc tgcgcattgg ccggctgatc ggcccgcagg cgcaattgct
     1381 caccggctcg atcgatctga tcatggcggg gaaccgcgtg gcgggggcgc gcaatgtcgt
     1441 caccgggctt gattcggccg ggcgcatcac cggcagctat gccaaggcgc atctggtgcc
     1501 ttatggcgaa tatctgccga tgcgctggct gctgaagccg ctggggctgg aacggttggt
     1561 gcccggcgat atggatttca cccccggccc cggccctgcc acgctggatc tgggcgtgct
     1621 gggcaaggcg ggcgtgcaga tctgctatga aatcatcttc cccggcgcgg tggtggatgg
     1681 cgcgcatcgg ccggagtata tcttcaaccc ctccaacgac ggctggtttg gcgattgggg
     1741 cccgccccag cacctggccc aggcgcggct ccgggccata gaggaaggcc tgccggtgct
     1801 gcgggccacc accaatggca tcagcgcggt ggtcgattcc cacggggtgg tggtgcaatc
     1861 ggccgggcgc gatgtggcgg caaggctgga tggcaccgtg ccgccggcgc tggcccccac
     1921 cccttttgcc agcgctggca acgcattgtc gcttggctgg gcattacttc ttcttgtcgc
     1981 ggcagcgctt gtgttgcggc ggcgggagcg ctagagagcc tcacataaag atttctttat
     2041 atgggcaggc gtcatgcgca gcgaatttct gtttacctcc gaaagcgttt ccgaaggcca
     2101 tcccgacaag gtggctgacc agatctccga tgccatcgtc gatctcttcc tcgccaagga
     2161 tccggaggcg cgcatcgcct gcgaaaccct caccaccacc cagctggtgg tgctggccgg
     2221 cgaaatccgc ggccgcggca tcatggacga ggccggcaac tgggcgcccg gcgtgcccga
     2281 ggaagtggaa gcggccgtgc gcgccacggt gaagcgtatc ggctacgagc aggagggctt
     2341 ccactgggaa agcctgcgct tcgagaacaa cctgcacccg caatcggcgc atatcgcgca
     2401 aggcgtggat gccggcgcca acaaggatga aggcgcgggc gatcagggca tcatgttcgg
     2461 ctttgcctgc gacgagacgc ccgagctgat gccggccacg ctctactaca gccacaagat
     2521 cctcgagcgc ctggcggccg accgccacgc cggcaccgcc cccttcctgg agcccgacgc
     2581 caagagccag gtgacgctgc gctatgagca gggcaagccg gtggcggcta ccgccatcgt
     2641 cgtctccact cagcacaagg ccggctatga cgaaggcgcc gggcaggctg agctcaacgc
     2701 ctatgtgaag caggtggtgg gcgagatcct gcccgcaggc ttcctgtcgg ccgacactgc
     2761 ctggcacatc aaccccaccg gcagcttcga gatcggcggc cccgatggcg atgccggcct
     2821 caccggccgc aagatcattg tggacaccta tggcggcgcg gcgccccatg gcggcggcgc
     2881 cttcagcggc aaggatccca ccaaggtgga ccgcagcgcc gcctatgtgg cgcgctatct
     2941 cgccaagaat gtggtggccg ccgggctggc ctcgcgcgtg accatccagc tttcctatgc
     3001 cattggcgtg gcccggccgc tgtcgctcta tgtcgacacg catggcaccg gcaaggccga
     3061 ggatgccgcg ctggaagcgg cgattgcccg cgtggcggaa gaaaagctgg gcggcctcac
     3121 cccgcgcggc atccgcctgg gcctgggcct caaccagccg atctacagcc ccagcgctgc
     3181 ctatggccat ttcggccgca ccgccgaggg cgcctatttc ccctgggaac gcaccgatct
     3241 ggccgaagcg ctggtggcgg cgctggcctg agggcgcaca tgcgcgtaga ttgacaaaag
     3301 ggggtgccgc aaggcgcccc ctttttgcat gcgggcagtt gatattcgtc atggttttcg
     3361 caaaaccttg cacgcacaag ggttagggcg ggacaagtga ctaacgagcg ggagatataa
     3421 tccatgcgtt cgattctggt gtttgccgat catgccgatc cggcgactga aacccggctg
     3481 gaaacggccc tgtcattggc gcgcagcagt ggcggccatg tcagcgtgct ggtcgatacg
     3541 ccgatcgccc gcttcatggc gatggatgcc atgggcggca gctatctggc cgccgatgcc
     3601 atccgcgatg cggtggagcg cgacgatgcc tatgccgagg ccctggccga gcggctgaag
     3661 cgcgaggacg tgcccttcga catccagcgc ggcgaggatg agccggtgga tgcgctggcc
     3721 gatgccagcc gcctggccga tgtcatcgtg ctgtcgcgcg ggtgcctgtt tgccgggcag
     3781 ctggcggtgt cggcgcgggc gcccattctg gtggtgccgg aaacgggcgt ggtgggcgtg
     3841 cccttcaccc gcgcggcggt ggcctgggat ggctcgcagg aagcggccgc ggcgatgaag
     3901 gcggcgctgc cgctgctggc ggcggcgggg caggcggatg tgctgatcgt cagcgccgag
     3961 gcggcaggct atccggcggt ggatgcggtg caatatctct cgcgccatgg cgtgcatgcc
     4021 gaattgcacg atctggcccg ggccggctcc accgagggca cgctggccgc cgaggtgcac
     4081 aagatcggcg ccgacctgct ggtgatgggc gcctatggcc acagccgttt ccgcgagttc
     4141 atcgtcgggg gcgtgtcgcg cgctttcctc gaaagcgagg gcgggccgac gctgctgctg
     4201 gcgcactgat ttaaccaaaa cctggttagc ggggcgagag ctccgatgaa gggcatggct
     4261 tgcgcaaggc gcagggcgtg ccctttgccg ttggccaggt tgcttttcac gcaaggcggc
     4321 aggggcgcgg gccgagaggc ccgggatagc tggcagatgg tggcgggggg cggtgttttc
     4381 cttgaggcag atggattgcg aatcgagaaa atggcctttt tggattttag aaattataca
     4441 attatccaaa taggaatatt tctgacgcac acgtgaggca ggcatacgta tgcatgaggg
     4501 attggcctgg cgatttgctc gggtacatgt ggctaataca atgaatttaa atttttttat
     4561 ttggtgtgcg cattgattgc gattcctgca acaaactccg ccattttcgc atttgcggcg
     4621 aatcggggat tcacccataa ggatcaggcc tttcaggcat cctctcccaa aacttccacg
     4681 acccggcttc ggccgggtct ttttttgccc gctgcggcga tggcggggca aggccttccg
     4741 gctggatgca caggcgtcgc cggtcacgaa ccgcaatttt ccatcccggg ggcgaagggg
     4801 gcgggcgttg gtgattcgcc ggcgtcagga tggtgattca ggccccttct tgtccctttg
     4861 gccgggttgc aatgggcaga cctatcacat tgtgcagtgc ggcatggtac aaggcggccc
     4921 ccttgaaaca ccagatcgga tgcacaaaat attttttaaa aacagatata tgaacggaat
     4981 agaatcagca ttcgtgccga tatcggtcgc gtgcatgcgc gcaaatcgat attttgcact
     5041 tgcgaagaag ggaggggtga ggcatataga aactgccttt caggcatcct ctcccaaaac
     5101 tttcaaagcc cggccagcaa tggccgggct ctttctttgg gcagggggca aagggcaggg
     5161 gcaggccctc aggccttatt ccgcctcctg gcacagggca tcgggggcgt tggcctgcag
     5221 ccagctcatc atcccctcgc gcacagccgg ccgcagggcg cccagcaggg tgggctgggc
     5281 cgcgctcacc agcaggcgca gttccacaac gcccggccgc acatcggcga tatagaggct
     5341 gcccacccgg ccgtcccatt ccggcatgct ctgcagcatg gcaaggaacg ccgcgcggat
     5401 cggcgcgata tcggtgcccg gtcgcaccgg gatcaccacc ggcccggcaa tcccgcccac
     5461 gcgtgaccag ttctggaagg tggcatcgag gaatttggtg ggcggcacga tcagccggcg
     5521 ctcatcgctg gtctgcacca ccacatagct gaggtggatg tcttccaccc gcccgctctc
     5581 gccatccacc accacataat cgcccaggcg aatgggttcg gtcagggcga tctgcagccc
     5641 cgcgatcagc gatttcagca tcggctgggc ggccgcgccc accgccagac ccagcaaacc
     5701 ggccgaggcc atcaacgtgg cgcccagatg cctgaccgcc ggaatgccga aaaggatcag
     5761 cgagagggtg atgaagatga tgacaaagcc ggccgagcgg gccagcaggg caatgcgcgt
     5821 gcgccggctg cggatggcca gttcgtcgtc cacccggtcg gcacgggccg ccagcacctc
     5881 ggcccccacg ctgaccagcg catagaccac ccagcccgtc agcgccggca tcacgaagcg
     5941 cgccaccgcc ccccacagat gcgccaccag ccaatcgcca tcatcggcga tggacagcgc
     6001 cacgcccacc agcagccagc gggtggaacg gtggagccgc cgggccgcgg gcgtgggctc
     6061 attgccctcg ccgcggcgca ccatgtgggc gatcatggcg aacaccagct tgtgcagcac
     6121 cagcgcgatg gccaccgcca cccccgccac gatggccgaa tgcgtgagcc ggtgcgacca
     6181 gtggaggaat tcgctgaacg agggcagcgc atcggtatcc agcgtgggcg tgggcagcgg
     6241 caccgggcca gaagcggcaa acagggggaa aggcatgaga gtcctctaaa aagggccggg
     6301 gggcaggctc aatgcgccgc gccccagctg gggccggtgc cgatctccac cgccagcggc
     6361 acgctgagcg ggctggaggg cagggcggcc tctgccatca cctggcggat caccgtgctg
     6421 gcggcaggca catcggcttc gggcagttcg aacaccagtt cgtcatgcac ctgcagcagc
     6481 atctgcacat ggccaaggcc ggcagcggcc agggcggctt ccatgcgcgc catcgcccgc
     6541 ttgatgatat cggcgcaggt gccctggatg ggggcgttga tggcggcgcg ctcgctgccc
     6601 tggcgctcgg cctggttctt ggcgctgatg cgggggaacc agcatttgcg gccgaacagc
     6661 gtttgcgaat agcccttctc gcgcacgctg gagagggttt cgtggatata gcgctggatg
     6721 ccggggaagc gctcgaaata gcggtcgatc agggcctggg cttcctcggg gggcacgccc
     6781 aggcgcggcg ccaggcccca gcgcgagatg ccgtagagga tggcgaaatt gatcgtcttg
     6841 gcgcgcccgc gcgtctcgcg ggtcacctcg ccgaacagtt cctgcgcggt ggcggcgtga
     6901 atgtcggcgc cggcggcaaa ggcggccttc agcgccggca catcggccat gtgggcggcc
     6961 aggcgcagct cgatctggct atagtcggcc gacagcagca catggccggc atcggccacg
     7021 aaacagtcgc ggatctggcg gccgatttcg gtgcggatgg ggatgttctg caaattgggc
     7081 tccgtggacg acaggcgccc cgtctgtgcc cccaccaggc tgtagctggt gtgaacgcgg
     7141 cccgtggcgg ggctgatcgc ggcctgcagc gcctctgtgt aggtggagcg caatttggcc
     7201 agctggcgcc attccagcac cttgccggca atcgccaccc cgtcgccctc cagcttttcc
     7261 agcaccgact gatcggtgga atactggccc gatttgccct tcttgccgcc cttcagcccc
     7321 atctggtcga acaggatctc gcccagctgc ttggggctgc cgatggtgaa gggggtgcct
     7381 gccagcgcgt ggatctcgcc ctccagcccg gcgatcgctt cggcaaactg ggcggaaagg
     7441 ccggccagcc gttccctgtc cacccggatg ccgcgccgct ccatgccggc caccaccggg
     7501 atcagcgggc ggtccacccg ttcatagatg cgcgtggcgg cttccccggc caggcgcggc
     7561 ttcagcaggc ggtgcagccg ccaggtcacc tcggcatcct cggcggcata ggcggtggcg
     7621 cggtccagcg gcacttcggc aaagctgatc tgcttcttgc cggtgccgca cacatccttg
     7681 aaggcgatgc agctgtggcc cagatggcgg cgggccagct cgtccatccc gtggccgcca
     7741 aagctgatgc cgcccggccc ttcctcgccg cggccggcat caaggtcgaa gctcagcacc
     7801 atcgtgtcgt cgatggggcc aacggcgatg ccttcccggg ccagcacgtt gagatcatat
     7861 ttggcgttct ggcccacttt cagcaccgcc gggttctcca gcagcggctt cagcagggcc
     7921 accgcctcgg ccttggcgat ctgccgcggg gtttcggcaa acatgtcggt gccgcgatgg
     7981 cccagcggga tgtagcaggc ctcgccgggg ccggtggcaa ggctgatgcc caccagatcg
     8041 gcgccgatgg catcaagcgc gctggtctcc gtgtcgaagg cgatgaggcg ggcggccagc
     8101 ccgcgcgcca cccagcgcgc cagcgcctcg gcgctttgca cgcattcata gcgggcatgg
     8161 tcgatcggcg gcagatcggc cagtggctgg cgggtgccgg cgggcgccgg ggcggccgcg
     8221 gcatgctcgg cctttgcggg gtgcaactgg gtggcgcgct cggggctgcc gcggccggca
     8281 tccagccgct tggccaggct ggtgaagcca tgggtgtgga ggaaagcggc cagcggcgcc
     8341 tcggggatct gccccagcgc gaaggcttcc agcggcagcg gcagcgcgca atcctccttc
     8401 agcgccacca gcacccggct gaggcgggcc atttcggcct gctcgatgag gttctcgcgc
     8461 agcttgctgg ccttcatggc gggggcagcg gccagcgcgc tttccaggtc gccatgttcc
     8521 tggatcagct tggtggcggt tttggggccg atgccgcgga tgccgggcac attgtccacc
     8581 gcatcgccca tcagcgccag cacatcgccc acctggcctg gcaccacgcc gaatttctcg
     8641 gccacttcgg gcaggaagat gcgctggttc ttcatcgtgt ccagcatgtc gatgcggctg
     8701 ccatcggggc catcgcccac cagctgcatc aggtccttgt cggaggagac gatggtcacg
     8761 ctccagccgc gggcttgcgc cgcgcgggca taggaggcga tgagatcatc cgcctccagt
     8821 ccttcctcct cgatgcaggg cagcgagaag gcacgcgtgg catcgcggat cagcgggaac
     8881 tgcggcacca gatcctcggg tgcgggcggg cggttggcct tgtaggcggg gtagatgtcg
     8941 ttgcggaagg agcggccggc cttgtcgagg ataacggcca gatgggtggg gccatcggcc
     9001 ttgtgcaggt cctcggccag cttccacagc atggtggtat agccatagac cgcgcccacc
     9061 ggcacccctt cggggttcgt cagcggcggc aggcggtgat aggcgcggaa gatataggcc
     9121 gatccgtcga ccagatagag atggggaggc ttgtccatgc ccgcctcata gggccacagg
     9181 ggcgccgcgc aaaggccggc ttgcagatgc gcggcgcaaa ggccggccag ggggtgggcg
     9241 gcttgcggcg attcggtatt ttcgggggtt gcggcttccc tttatgccgc aatgcggcga
     9301 caacgcggcg tcagggatcc gccattcagg tgcagaatcg cgcggttcgt cgtggtcaca
     9361 agcgactagc gcttgcatcg gctgcgcaag ggcattatct gacaggcgaa gtctactcct
     9421 gcggtaggct ttccgcacgg cgcgccacgc tttgtggacg gtgtcgcgcg tggtccactc
     9481 gctgttcaag gaataccatt catgcgcaag atcattctcg ctgctgccgt tgctggcgct
     9541 gctctgagcc tggccgcttg caagggcggc gaagccgctt cgtcggctga caccgctgtg
     9601 tcggctgatt cggctgtgtc ggctgattcg gctgtttcgg acgttgccac cggcgtggac
     9661 tcggctgctt cggctgctgc ctcggccgct gcttcggcca ccagcaagta agctttcgca
     9721 tcccgcgagg gtttcggaaa agggcgcgtg gacaagctcc gcgtgccctt tttctttgcc
     9781 cgcggggcag gcgcctgaaa gggcgcacag aaaacgggcg agaggcacaa ggccccccgc
     9841 ccgttttctg tgcgcccttt gccgtccgcc gcagccggcc tcagttcagg ctgacgcggc
     9901 tgccgccctg cgccggatcg gagaaattgt cgtacaccgc gcgggcgatg gtggcgatgc
     9961 gcgattcccg ccccggccgg ctgccctggc cggtaacata gatcgccagc gccaccgtgt
    10021 gcccatcggc ggtgcggatg aagccaacgt cgctggaggt gttgctcagc gtgccggtct
    10081 tgtggccgat gatcgcttcg tcgggcacca gcgccttcat gcggtgcacg ccggtcaggc
    10141 agcggctcat cgcgcccagc agcacttcgc ggctggccat gctcagccac tggccctgat
    10201 agaggccggc cagcagcgtg gccatggcct ggggcgtggc actgtcgcgc aggtcgatcg
    10261 ttgtcgccgg atccaccccg ccatcgtcgc gcaccagcgt ggcgatatcg cggttcacat
    10321 ggatgccacc gatcttggcg cgatgcagcc agcggtccac cgcctgcggc ccgccgatgg
    10381 cggccagcag cgcgtcggtc gcgcggttgt cgctgcgggt gatggccagc tcgatcagtt
    10441 ccagcgccga cagcgcctgg ccggcatgca ccggcgccac gctgctggag aacttgcgcg
    10501 agggcaccgg gatcatcagc ggaaagcggt cggtcaggcg gaagcggccc tggtccaccc
    10561 cttcgaggaa agtggcgacg atcgccactt tcaccgtgct ggccatgggg aagggctgat
    10621 cgcccatcac cgagacgccc gagccagtgg agagatccag cgctgccacg ccaatgcggc
    10681 cgtgggcggc attggccagg cccgccagcc gcgcctcgaa ggaagaggcc gggcccaccg
    10741 ggagcgctgc caccggcgct gccggagcgg gcgccggacg ggcggccggc tgcgcgccga
    10801 acaccttgtc gaaggcctgt tccagatcat cggtggcaag ggccggcggc gcgatcagcg
    10861 cacaagccat gaaggcggcg aacaggaggt gctgcttggt gtgtttcatc gcacgctgga
    10921 gggtaaaagg aaaactttaa gcaacatttg ctgcaaaccc cgcttttcgc caagcccccg
    10981 ggcggtgaaa tggttagggc ttgcgatagc cggcgcgatg ggccgtgcca aataggggat
    11041 taccggtgcc ggccggcaca acaaaggccc ggaaagggat cgctcccctc cgggccttcg
    11101 ctgttcaagg cagggtggac cggcttagaa gtccatgccg cccatgccgc cgcccggcat
    11161 cgcgggcatg gcgggcttgt cttccggctt ctcgacgatg ccggcctcgg tggtgatcag
    11221 caggcccgac accgaagcgg cgtcctggag ggcggtgcgc acaaccttgg tcggatcgat
    11281 cacgccggcg gccagcaggt tctcatacac gtcggtagcc gcgttgaagc ccagggcctc
    11341 gtcggcctgg tcgagcagct tgccggcaac cacggcgcca tcatggccgg cgttctcgct
    11401 gatctgcttg atcggcgcgg taaccgcctt gcggatgatg tcgatgccgc gggtctggtc
    11461 gtcgttggcg ccggccaggc cagccagggc cttggtggca tagagcagcg cggtgccgcc
    11521 gccgggcacg atgccctctt ccacggccgc gcgggtggcg tgcagggcat cgtccacgcg
    11581 atccttgcgc tccttcacct caacctcggt ggcgccgcca accttgatga cggccacgcc
    11641 gccagccagc ttggccaggc gctcctgcag cttctcgcgg tcgtagtccg aggtggtgat
    11701 ctcgatctgc gcgcggatct gctcgacgcg ggccttgatc tcctcggccg aacccagacc
    11761 atcgacgatg gtggtgttgt ccttgtcgat cgtcaccttc ttggcctggc cgagcatgcc
    11821 cagcgtgacc gattccagct tgatgcccag gtcttccgag atcatctcgc cggcggtcag
    11881 cgtggcgata tcgcccagca tggccttgcg gcggtcgccg aagccgggcg ccttgacggc
    11941 ggcgatcttg aggccaccgc gcagcttgtt gacgaccagc gtggccagcg cctcaccctc
    12001 gatgtcctcg gcgatgatca gcagcggacg gcccgactgc accaccgctt ccaggatcgg
    12061 cagcagcgtc tgcagcgacg acagcttctt ctcgtggatg aggatgtagg gcgattccag
    12121 ctcgaccgtc atcttctcgg ggttcgtcac gaaatagggc gacagatagc cgcggtcgaa
    12181 ctgcatgcct tccacgacat ccagctcgaa ttcgaggccc ttggcctctt ccacggtgat
    12241 cacgccttcc ttgccgacct tctccatggc ctcggcgatc ttctcgccga cttcacggtc
    12301 gccattggcc gagatgatgc ccacctgggc gatctgggcc gaatcagcca ccggcttcga
    12361 gcgggccttg aggttttcca ccacggcggc cacggcgatg tcgatgccgc gcttcaggtc
    12421 catcgggttg atgccggcgg caaccgactt caggccttcg cgcacgatcg cctgggccag
    12481 cacggtggcg gtggtggtgc cgtcaccggc cttgtcgttg gccttgctgg ccacttcgcg
    12541 cagcatctgg gcgccgaggt tctcgaagcg gtccttcagc tcgatttcct tggccaccga
    12601 gacgccgtcc ttggtgatgc gcggggcacc gaacgacttt tcgatcacca cgttgcggcc
    12661 cttggggccc agcgtcacct tcacggcgtc ggcgagggtg tccacgccct tgaggatacg
    12721 ttcgcgcgcg tcgcggccga acttcacgtc cttggctgcc attgttctat cctttgaaaa
    12781 tcagaaacgg ggaggaagac agtctggtcc ggctgatcag ccgatgatgc ccaggatgtc
    12841 gctttccttc atgatcagca ggtcttcgcc gccaaccttc acttcggtgc ccgaccactt
    12901 gccgaacagc acgcgatcgc ccgccttcac ttccagcggg gtgacggtgc cgttctcggc
    12961 gcgggcgccg gtgcccaccg agacgatcac gccctcggcg ggcttttcct tggcgctgtc
    13021 ggggatgatg atgccgccgg cggtcttttc ctcggcttcg acacgacgga ccagaacgcg
    13081 gtcgtgcagg ggacggaacg acatgggagt gttacctctc tcaagtggaa aacaggtctt
    13141 ggcactctgg cattgagagt gccagcagcc gggatatggg gcagccggcg ggggccatca
    13201 agggggcttg cgcaaatttt tgtggcggcc ggcgcgccgc atcgggcggc ggcgcaaaat
    13261 cgg
//
LOCUS       DASRYE010000125         5974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_236431,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000125 DASRYE010000000
VERSION     DASRYE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236431"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFF98_07000"
     CDS             complement(<1..217)
                     /locus_tag="VFF98_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63411.1"
                     /translation="MTGKRVTTGRQVAQRKLLRAALMAGLAGLLLAGSAEAAGSARAV
                     GQGPEPLLPPTPPTPRPTPSTAATQKAF"
     gene            409..1350
                     /locus_tag="VFF98_07005"
     CDS             409..1350
                     /locus_tag="VFF98_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU63412.1"
                     /translation="MPALPFPRLPFPARLRPFLPALACGLGIACFSVMDALMKQSSLS
                     AGVFTAVFWRTAIGTAIATPLWLTSGKARWPGRGMLALHGLRTLAVAGMVLLFFWGLV
                     RTPMAVGMALSFIAPLIALLLAALFLGEPVTRRALAASALGLAGVVVIGAGRLGAGPT
                     RGDSLAGMVAILGSAVLYAVNLVIQRHQAQQVSGREIVFFQNALFVVALLPAVPFFGH
                     LPHGHIWLLIALAACLALTSILLLSWGWARAEAHRLLPVEYSAFLWSALMGRWWFGEA
                     LGLPTLAGVVLIVAGCWIGASAGAGEEGPPHIEQTAL"
     gene            1553..4222
                     /gene="acnA"
                     /locus_tag="VFF98_07010"
     CDS             1553..4222
                     /gene="acnA"
                     /locus_tag="VFF98_07010"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZU63413.1"
                     /translation="MTQVGQDSLGTRSTLSAGGKTVAYYSFKKAAEKIGDVSGLPFSM
                     KVLLENLLRFEDGGFTVSVDDIKAIADWQTNPRSEREIQYRPARVLLQDFTGVPCVVD
                     LAAMRDAIAKLGGDTSKINPLVPVNLVIDHSVMVDEFGHPKAFEQNVEIEYHRNMERY
                     DFLKWGSKSLNNFYAVPPGTGICHQVNLENIAQTVWTSEDQNGQIVAYPDTCVGTDSH
                     TTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFKFTGALKEGVTATDLVLTCT
                     NMLRKKGVVGRFVEYYGPGLAGLTLADRATLANMAPEYGATCGFFGIDDKTLDYMRLT
                     GREEDQIALVEAYAKEQGFWIDPAIEPVFTDTLELDLSTVVPSLAGPKRPQDRVSLPD
                     VDDVFNADMANVYKKTQARVPVEGKDFDIGDGDVTIAAITSCTNTSNPGVLVAAGLVA
                     KKANELGLKPKPWVKTSLAPGSQVVTDYLNKAGLQEHLDAVGFNLVGYGCTTCIGNSG
                     PLAEPISKAINENGLVAAAVISGNRNFEGRVSPDVRANFLASPPLVVAYALKGTVIED
                     FTTTPIGKGKDGQDVFLKDIWPTNEEVHTTMAGCMDRPMFQARYADVYKGDKHWQAIN
                     VTGSETYSWRAGSTYVANPPYFEGMSMTPAPVVDIVEAKPLLILGDSITTDHISPAGN
                     IKADSPAGQWLMEHQVAKADFNSYGARRGHHEVMMRGTFANIRIKNEMVPGIEGGFSR
                     YGEEVGAVYDVAQKYKADGTPLVVIAGKEYGTGSSRDWAAKGTNLLGVRAVIVESFER
                     IHRSNLVGMGVLPLQFKAGDTRETLGLTGDDSFTIKGLASIAPRQDVTVEVTRKDGST
                     FTFTALCRIDTANEVEYFNNGGILQYVLRKLAA"
     gene            complement(4337..5128)
                     /locus_tag="VFF98_07015"
     CDS             complement(4337..5128)
                     /locus_tag="VFF98_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444086.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZU63414.1"
                     /translation="MSVRMGIAKLATVVAGGALIGGGAVHVAEQLRPGALEYVKHGKG
                     APSKLVRIRKVEARPTKLVAIAPMPAPAPEVTPAPTPQPGLPAANFDVSGGQDATYVA
                     DAAPARSDGGFSGGFFGGFFGGGGGGSTVVVVSSGGSSTSTSTSTSSSTSTSTSSSTS
                     SSTSSSTSGGATSTSSTSGGTSSSTSGGSSTGSSSSTTSGGATSSTSSTSGGTSTTST
                     SSSSSGGSTSTTSGGTQVPAPPMMALFGAGAAAMLLRRRKGAGGA"
     gene            complement(5360..>5974)
                     /locus_tag="VFF98_07020"
     CDS             complement(5360..>5974)
                     /locus_tag="VFF98_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HZU63415.1"
                     /translation="RIDLPPFTLVGATTRQGLLTTPLRDRFGIPVRLQFYTVDELERV
                     ITRGAGLLGIGIEREGAREIARRARGTPRVAGRLLRRVRDFAHVAGSTTITCAIADDS
                     LTRLEVDSLGLDAQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVVEPYLIQL
                     GLVARTARGRCLNDGGWRHLGLAPPNGQMPQGGLFDMGEDGPAR"
BASE COUNT          947 a         2055 c         1997 g          975 t
ORIGIN      
        1 caaacgcctt ctgggtggca gccgtgctgg gcgtggggcg cggcgtgggc ggagtgggtg
       61 gcagcagcgg ctctggcccc tggcccacgg cccgggcgct gccggcggcc tcggcgctgc
      121 cagccagcag caggccggcc agccccgcca tcagtgccgc gcgcaaaagc ttgcgctggg
      181 ccacctgcct gcctgtcgtc acccgcttgc ctgtcatcga acctgtgtcc tcgcaccgcg
      241 aaattgcctg ggccggcagc gcctgggggg ctggccggcc gccagccccc tcccttgccc
      301 atttcggcag gggccatcaa gccttatgcg tgacagcgcg aagcgttatg cgtgaccacg
      361 cgcagcctta tgcgtgacgg cggcggcgct gccggctagg gctggcgcat gcctgccctg
      421 cccttccccc gcctgccctt ccccgcgcgc ctgcgcccgt tcctgccggc gctggcctgc
      481 ggcctgggca ttgcctgctt cagcgtgatg gatgcgctga tgaagcaatc ctccctttcg
      541 gccggcgtgt tcaccgcggt gttctggcgc acggcgatcg gcacggcgat tgccacgccg
      601 ctgtggctca cctcgggcaa ggcgcgctgg ccggggcggg ggatgctggc gctgcacggg
      661 ctgcgcacgc tggcggtggc ggggatggtg ctgctgttct tctgggggct ggtgcgcacg
      721 ccgatggcgg tgggcatggc gctgtcgttc attgcgccgc tgatcgcgct gctgctggcc
      781 gcgctgttcc tgggcgagcc ggtgacgcgg cgggcgctgg cggcctcggc gctggggctg
      841 gcgggcgtgg tggtgatcgg cgccggccgg ctgggcgccg ggcccaccag gggcgattcg
      901 ctggccggca tggtggcgat tctcggctcg gcggtgctgt atgcggtcaa cctcgtcatc
      961 cagcggcacc aggcgcagca ggtttccggc cgcgagatcg tgtttttcca gaatgcgctg
     1021 ttcgtcgtgg cgctgctgcc ggcggtgccg tttttcgggc acttgcccca tggccacatc
     1081 tggctgctga tcgcgctggc ggcctgcctg gcgctcacct cgatcctgct gctcagctgg
     1141 ggctgggcgc gggccgaggc gcaccggctg ttgccggtgg aatacagcgc cttcctgtgg
     1201 tcggcgctga tgggccggtg gtggtttggc gaggcgctgg gcctgccaac gctggccggc
     1261 gtggtgctga tcgtcgccgg ctgctggatc ggcgccagtg ccggggccgg cgaagagggc
     1321 ccgccgcata tcgaacagac cgccctctag gcgtttttgc aataacgctt cgcaatagcg
     1381 aaaaagcgcg gccaagcttg caaaattagc cattaggcgg ccttgacgcg gagcccaaac
     1441 ccgcgcatcg gcccgcttgc aacaatcgct tttccgcgca ggccgccggg gggccaacca
     1501 gctgcccgaa tgccacaagc ggagaagcct tcagcttgag gatacacgcc gcatgaccca
     1561 ggtcggacag gactccctcg gcacccgcag cacgctcagc gcgggcggca agacggtcgc
     1621 ctattattcc ttcaagaagg ccgccgaaaa gatcggcgat gtctcggggc tgcccttcag
     1681 catgaaggtg ctgctggaaa acctgctgcg cttcgaagat ggcggcttca ccgtctcggt
     1741 ggacgatatc aaggcgattg ccgactggca gaccaacccc cgctcggaac gcgagatcca
     1801 gtatcgcccg gcccgcgtgc tgctgcagga tttcaccggc gtgccctgcg tggtggacct
     1861 tgccgccatg cgcgatgcga tcgccaagct gggcggtgat accagcaaga tcaacccgct
     1921 ggtgccggtc aacctggtca tcgaccactc ggtgatggtg gacgaattcg gccaccccaa
     1981 ggcgttcgag cagaacgtcg agatcgaata tcaccgcaac atggagcgtt atgacttcct
     2041 caagtggggg tcgaagtcgc tcaacaattt ctacgccgtg ccgccgggca ccggcatctg
     2101 ccaccaggtg aacctggaaa acatcgccca gacggtgtgg accagcgagg accagaacgg
     2161 ccagatcgtc gcctatcccg acacttgcgt gggcactgat tcgcacacca ccatggtcaa
     2221 cggcctgggc gtgctgggtt ggggcgtggg cggcatcgag gccgaggccg cgatgctggg
     2281 ccagccggtt tccatgctga tccccgaagt ggtgggcttc aagttcaccg gcgcgctgaa
     2341 ggaaggcgtg accgccaccg acctcgtgct gacctgcacc aacatgctgc gcaagaaggg
     2401 cgtggtgggc cgcttcgtgg aatattacgg ccccggcctt gccggcctga cgctggccga
     2461 ccgcgccacg ctggccaaca tggcgccgga atatggcgcc acctgcggct tcttcggcat
     2521 tgacgacaag acgctggact acatgcgcct gaccggccgc gaggaagacc agatcgccct
     2581 cgtcgaagcc tatgccaagg agcagggctt ctggatcgac ccggccatcg agcccgtctt
     2641 caccgacacg ctcgagcttg acctgtcgac cgtggtgccc tcgctcgccg gccccaagcg
     2701 cccgcaggac cgcgtgtcgc tgcccgacgt ggacgatgtc ttcaacgccg acatggccaa
     2761 tgtctacaag aagacccagg cccgcgtgcc ggtggaaggc aaggacttcg acatcggcga
     2821 cggtgacgtg accatcgctg ccatcaccag ctgcaccaac acctcgaacc cgggcgtgct
     2881 ggtggctgcc ggcctcgttg ccaagaaggc caacgagctg ggcctgaagc ccaagccctg
     2941 ggtgaagacc tcgctcgccc ccggctcgca ggttgtcacc gactacctga acaaggccgg
     3001 cctgcaggag cacctcgacg cggtgggctt caacctcgtc ggctatggct gcaccacctg
     3061 catcggcaac tcgggcccgc tggctgaacc catcagcaag gcgatcaacg agaacggcct
     3121 ggttgctgcc gccgtcatct cgggcaaccg caactttgaa ggccgcgtgt cgcccgatgt
     3181 gcgcgccaac ttcttggcct cgccgccgct ggtggtggcc tatgcgctga agggcacggt
     3241 gatcgaggac ttcaccacca cccccatcgg caagggcaag gacggccagg acgtgttcct
     3301 gaaggacatc tggccgacca acgaggaagt ccacaccacc atggccggtt gcatggaccg
     3361 cccgatgttc caggcccgct atgccgacgt ctacaagggc gacaagcact ggcaggcgat
     3421 caacgtcacc ggttcggaaa cctatagctg gcgcgccggt tccacctatg tggccaaccc
     3481 gccctacttc gagggcatga gcatgacgcc ggccccggtg gtcgacattg tcgaggccaa
     3541 gccgctgctg atcctgggcg attcgatcac taccgatcac atcagcccgg ccggcaacat
     3601 caaggccgac agccccgccg gccagtggct gatggagcat caggtcgcca aggcggactt
     3661 caactcgtat ggcgcgcgcc gcggccacca cgaagtgatg atgcgcggca ccttcgccaa
     3721 catccgcatc aagaacgaga tggtgcccgg catcgagggc ggcttctcgc gctatggcga
     3781 ggaagtcggc gcggtctatg acgtggcgca gaagtacaag gccgatggca cgccgctggt
     3841 ggtgatcgcc ggcaaggaat atggcaccgg ctccagccgc gactgggcgg ccaagggcac
     3901 caacctcctg ggcgtgcgcg ctgtcatcgt ggaaagcttc gagcgtatcc accgctcgaa
     3961 cctcgtcggc atgggcgtgc tgcccctgca gttcaaggct ggcgacaccc gcgagaccct
     4021 gggcctgacc ggtgacgaca gcttcaccat caagggcctg gccagcatcg ccccccgcca
     4081 ggacgtgacc gtggaagtga cccgcaagga cggctccacc ttcaccttca ccgccctgtg
     4141 ccgcatcgat accgccaacg aagtggaata cttcaacaac ggcggcatcc tgcaatacgt
     4201 gctgcgcaag ctggccgcct gagccagact gccagattga aaacaggaaa gggcggcctt
     4261 gcggggccgc ccttttcgtt tgcctgccgg aaaaaacgcc gccgcggaaa atagcggcgc
     4321 gccgggcctg ccggggttat gcgcccccgg cccccttgcg ccggcgcagc agcatcgccg
     4381 ccgcgcccgc gccaaacagc gccatcatcg gcggggcggg cacctgcgtg ccgccgctgg
     4441 tggtggacgt gctgccgcca gagctgctgc ttgatgtgct ggtggtgctg gtgccgccgc
     4501 tcgtgctgct ggtgctgctg gtcgccccgc ccgaagtggt ggagctgctg gagccggtgc
     4561 tcgatccgcc gctggtggaa gagctggtgc cgccgctggt cgacgaggtg ctggtcgccc
     4621 cgcccgaggt ggagctgctg gtcgagctcg acgtgctgct cgatgtcgag gtggacgtgc
     4681 tgctcgacgt ggaagtggac gtgctggtgg acgagccgcc gctgctgacg acgaccaccg
     4741 tgctgccccc gccgccgccg ccaaagaacc cgccgaagaa gccgcccgaa aagccgccat
     4801 cgctgcgcgc gggcgcggca tcggccacat aggtggcatc ctgcccgccc gacacgtcga
     4861 aattggcggc cggcaggccc ggctggggcg tgggcgccgg ggtcacttca ggcgcgggcg
     4921 cgggcatggg ggcaatcgcc accagcttgg tggggcgggc ctccaccttg cggatgcgca
     4981 ccagcttcga gggcgccccc ttgccgtgct tcacatattc cagcgcgccg gggcgcagtt
     5041 gctcggccac atgcacagcg ccgccgccga tcagcgcgcc gccagccacc accgtggcca
     5101 gtttcgcaat gcccattctc accgacatag ccgttgcttt ccgttcaacc ccgcgccgcc
     5161 cgcgtgccgc cggccttcgc gccggcagcg cagctcttgc gccatgtgtg gttaaaaact
     5221 gcgcaacaca tgggcgaagc gcaaggattc gcccggcctc aaagctgtgc cttttccgga
     5281 ttccgcttcg atttcagggt ttggcgccaa caatgtcccg tttcgggaca ggttttgccg
     5341 cctttgccaa accgcccggt taacgcgccg ggccatcctc gcccatgtcg aacaggccgc
     5401 cctggggcat ctggccattg ggcggggcca gccccagatg gcgccagccg ccatcgttga
     5461 ggcagcgccc ccgcgccgtg cgcgccacca gccccagctg gatgagatag ggctccacca
     5521 cttcctcgat cgtgtcgcgc ggttcggaaa gaccggcggc cagcgtttcc acccccaccg
     5581 gcccgccctt gtagatatcg gcgatcatgg tgagatagcg cctgtcctgc gcgtcgaggc
     5641 caaggctgtc cacctccagc cgggtcagcg aatcatcggc gatggcgcag gtgatggtgg
     5701 tgctgcccgc cacatgggca aaatcgcgca cccggcgcag caggcgcccg gccacgcgcg
     5761 gcgtgccccg cgcccggcgg gcgatctcgc gcgcgccctc gcgctctatg ccaatgccca
     5821 gcaggcccgc gccgcgggtg atcacccgct ccagctcgtc caccgtgtag aactgcaagc
     5881 gcaccggaat gccgaaacga tcgcgcagcg gggtggtcaa caggccctgc cgggtggtgg
     5941 cacccaccag cgtgaagggc ggcagatcga tccg
//
LOCUS       DASRYE010000126        20975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_162962,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000126 DASRYE010000000
VERSION     DASRYE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162962"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..847)
                     /locus_tag="VFF98_07025"
     CDS             complement(26..847)
                     /locus_tag="VFF98_07025"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZU63416.1"
                     /translation="MTIGVTEPVEPRAPASPYRAVSAEEIAHYQAHGWVRLRGFLPGA
                     DVAAMLAVARARMGEDADRDLAPGAFSYFNALPIHGVGQPVFDPVLRHYARAAVALRA
                     LRRPVGARYFLDFFGVKLPLAPGEGNGRSDWHQDFAAQVADRSGGMVFWTPLTDMTAA
                     HGTMAYLSGSHRHGVMGDYRSYGDGNLLDVYPELADACPSSGQLALLAGDVVVHSDLC
                     VHMAGANTAGTPRWTYLAIMNPADARWTGAPSVAFDTAGLTHLGELDDARFPRLA"
     gene            complement(1031..3124)
                     /locus_tag="VFF98_07030"
     CDS             complement(1031..3124)
                     /locus_tag="VFF98_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63417.1"
                     /translation="MRTRFLCATGMMGVLASPALALADAPALAMADAPAADAPPPGDG
                     GLREIIVTAQHRSENIQRTPIAITALSGATLRDEAKNTIAAAISATPAVVIQGNANGA
                     QIYVRGVGSNGDSQVGDAAVNLNIDGVYQQETEVPTSLMFDVDRVEVLRGPQGTLYGR
                     NANAGAINVLTADPSLAGPSGYATLQAGNYDAIHSEAAVNLPLGSSLALRGAFASERH
                     SGYLSNGNDDADVKAGRLKLLYKPGSALRVVLAGDYLHNAGNGLGTVDAPLSGHAPWY
                     STKPTGYLRVDAWNVRGQVDYTTAAANLTLLASHSFFSKDEANVLVGTPGVGDHRTGR
                     QDSVELRAASPDRSAIKWVAGLYYLSGMERRQAAAEPVSFALEAATDPEMRDAGTSSW
                     AAFANVTVPLAHAIRLTGGLRYTGDEKSARFYYANAALGTAYSSTRWSSLTYKAELEA
                     DLSAASMAYAQVSSGFKAGGYGQQFPVATYNPEKITSFEIGSKNRFWGNRLQLNASAF
                     YYRYSNYQASYADIVSGVFAFVTQNASSAQIYGLEAEGRLRLSANDTLGASLAYLHSR
                     FGAFVYTSALAGTVDHSGQTLPNSPALTVDLNYEHQFHLANQALLTAHVDTHVSQGYW
                     VTVERTLDSWQPGFTRSNAFLSYTPAGQVWSARLFVRNLENTAVRTLAQANPYYSVLM
                     LAAPRTYGGALTVHF"
     gene            3430..3861
                     /locus_tag="VFF98_07035"
     CDS             3430..3861
                     /locus_tag="VFF98_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63418.1"
                     /translation="MQIMERCRFDAYIARFNAKDASAFDEYLSPDMRLRNGRLEYTGI
                     EGMKRHYAAIWACMDETLDVLDYVTDGARAAVVMHTHFAVRTDAATSPFGPLRQGEAF
                     DYHGVIFYEITRGRFSRIIVSYLDFVKTDTDGRQTSLGIVH"
     gene            complement(3892..4797)
                     /locus_tag="VFF98_07040"
     CDS             complement(3892..4797)
                     /locus_tag="VFF98_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63419.1"
                     /translation="MRFHRLDLNLLIALRALLEEQSVRLASERLNLSQSATSSALGRL
                     RDYFEDDLLELKGRNLVLTARGQELLDPVRAILDQIQATMAVKQPFDPGVSDRRLSVL
                     ASDYAIEVMLGPAMAAFEEEAPHMVFEVKALGDEVVEELRRGRADLLLALDSVISTDM
                     PFTELWTDDFVVVCWSGNRALRQGISRAEYEEMHHVVTRFGWTRVPSFEEWALKSQSV
                     KRQVEVVAPSFTAVPSFLVGTQRIATMHRKLAERMARSLPLRILECPMDIPGIRVTAQ
                     WTPNGSNDPALMWLVGRLRAYAGQI"
     gene            complement(4901..5308)
                     /locus_tag="VFF98_07045"
     CDS             complement(4901..5308)
                     /locus_tag="VFF98_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU63420.1"
                     /translation="MPQFRFHHGAISVPDLDRAIAWYGETLGYTLEKRFSVPPAHSRA
                     AMLRKDDMRVEVFEIAGAAPLPPERRSVPGDLGVHGNKHVAYRVADFEAALAQFEAGG
                     VEIVFVVREAFGKGFFIRDCAGNLIEFVEEQAG"
     gene            complement(5399..6613)
                     /locus_tag="VFF98_07050"
     CDS             complement(5399..6613)
                     /locus_tag="VFF98_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63421.1"
                     /translation="MAAAHNRARTHRPGFLTGLSLLIPITLSTMAIVLLAPILPRMME
                     EFRQGPHADYWVPLVLTFPAICIALLSPVAGMLGDYFGRRRLLLCSFLGYAVVGIAPL
                     FLVSLPAILASRVLVGVFEALIMVLTTTMIGDYFDGPARDKWLAAQAATASLSALLFF
                     NIGGQLGAFGWRTPFWIYLSALAMFAMVLRFTWEPAEENAASEADHAPHHASWAHFPL
                     ARFVPVLLVTIYSSVFFYTVQIQASVGLAQLGLTDPARIGFLTSIASVGVPLGTVVYS
                     RSGLKDPRWLLLIEMVMLCTGFLVMARAQGPTPFLVGCFINQLGAGMLLPTLLVWAMG
                     LLAFEVRGRGSGLWQGALAVGQFLCPVVVTYLANRFGGLFAAFGCLAVGAGAGAVVAL
                     ATLAWQRKPAAA"
     gene            complement(6726..7400)
                     /locus_tag="VFF98_07055"
     CDS             complement(6726..7400)
                     /locus_tag="VFF98_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZU63422.1"
                     /translation="MTTSADAIRYTRMAVLLHWAIALMIGCNLTLGWFMEGFAPPLKG
                     VVLPLHISCGLSVLALSVVRIVWRLLHVPPPAPVGRLEQGAAHIVHFLLYAGMVVLPL
                     TGWAILSAHPAPGSQGFAAEQARMAAQAGPAVHRLRPKPGITAWWVIPVPAIGLVQAV
                     GDTPGGLPAQKRLHDSFVDWHEAGGWAMIALLALHIAGALKHQWVDRQPFLQRMSWQR
                     MSWRRR"
     gene            complement(7491..8282)
                     /locus_tag="VFF98_07060"
     CDS             complement(7491..8282)
                     /locus_tag="VFF98_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63423.1"
                     /translation="MKKLMLLGASLLSAVVLAGGARAAGTDACGKPITPTKQIQRLSD
                     IAEITNVSSSHEYYHGALMHQAEIENAWSKREDITWTNGTDKYANRTSLWKFYVDNLK
                     NFPHKGALWYHMLTTPYIEVAADGKTAKGIFMSFGNVSGEMHAGQPMSQWTEEKYGMD
                     FIKEDGHWKIWHLRTYVEFYVDVNKLWTDSRNNLAAVDTLALEGKNPDGSLRAGVQEE
                     AGSTFYGVKPDEKKIFYPGYTTSRDPQYNPAIPKPYCTFSQVEAY"
     gene            complement(8497..10704)
                     /locus_tag="VFF98_07065"
     CDS             complement(8497..10704)
                     /locus_tag="VFF98_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63424.1"
                     /translation="MAVYTNLSRSIAGTRLALQLGVGLAAVAFGGSAQAQNQPAATGA
                     ATPAPAGSNQEIIVTAQKRSEKLQNVPISIAAVSGPQLAKSGITEVAQLQQSVPALRL
                     NYSGNTVQPSIRGIGSSVAGPGLYSNIPVYVDGYYISSPTSSDIDLIDVDSVSVLKGP
                     QGTLFGRNATGGAIQITTRAPQHETQATFKLGYESFSHSTAAFYGTTGLTDTLAISLA
                     ADYEHSNGYITNILNGNDHAGEYRKWTVRPKLLWTPTSNLSFTLGYAHTYSDDPLLNS
                     TNVRVDGSGKPMTAGNVVPGLTIATAPNQISPGATNANKIITDAVTLTSVLKMSWADL
                     TSYTGYRKDYVAQALEYDDTTAAIDAAAWTIPDKSFTQEFNLTSKPGGRLSWVLGAFY
                     YWASDVYDYNLATTAAGAPYFHLFTSKNTTNSYAVFADVTYQLLDKLFLTAGGRYSKD
                     DLGLWFNLLGTTASGSTSFNNFSPRGVIRYQLTPKSNIYASYTRGYKSGALPGSAFSF
                     TPVKPEKIDAFELGYKVASGPLRFNLATYYYNYRDVQVASFFASGVSVVRNAASEHIY
                     GVDGDLSYAITPDFHVTLSGAYTHAVYGRFENAIGYQQDLNTSSATYSLFEPYNVNAT
                     GYPVQNTPRFAGSIGADYGFDLPKGRLVLNGNLFYSDGFYFDQAMQLPQTSYTLLNLR
                     ATWTDPSKKLDLSVYANNVTNQMYRIANFTDTFASRQVWGAPRTIGGSLTYRY"
     gene            complement(10959..12416)
                     /locus_tag="VFF98_07070"
     CDS             complement(10959..12416)
                     /locus_tag="VFF98_07070"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU63425.1"
                     /translation="MADISPVFARAAMLIDGEWVAAASGRSQLHHDPSTGLPLGAFAL
                     AGAVDIDRAIASCQRAFPAWRDAQPAFRRDVLLRAAAAIRARGAQFAAIAARESGAVY
                     APQAAGQAAEHLVYYAGWADKIEGSTVALGPGAFNYGLLEPYGVVAAINAWNGPLATA
                     LMKLGAILAAGNCVVLKAPDLGPFATLLLGEVMLEAGLPPGVLNVVTGLAEAGAALVA
                     DPRIGKISFTGGLPTARKVMAAAAVHATPVVSELGGKSANLVFADADLDRAAGMAARM
                     GCLTHAGQGCVLPTRLLVEEAVYDVFVEKVLAIVRAARVGHPCTPGVAYGPLINAAAL
                     ERVLAVVARAQQQGHGRLLAGGHRLGGELAQGYFMAPTVFGDVDNRSPLAQEEIFGPV
                     LSIIRFSGEAQGLALANDTPFGLAGYIHTTDLERAHRVAARLEAGYISINGFAPLPAP
                     APFGGYKQSGFGREGGREGLAEFLRHKNVYLELQR"
     gene            complement(12450..13670)
                     /locus_tag="VFF98_07075"
     CDS             complement(12450..13670)
                     /locus_tag="VFF98_07075"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZU63426.1"
                     /translation="MAGYDFLKTVTVLEVAQLGMDALGGHLADMGARVIKVEALPGGD
                     PVRFTGEHALGGPDGVGLLHLRWNRGKQSLGLDLKTPQGQALFRDLASRADVVIEGLK
                     GGALERLGLGYEVLRQRRPALVFCAISGLGRTGPYHALRSHAVAYDAYAGLVPADVDS
                     PPPAAHAFRPPSIGMQAPGLFAAVGVLAALLRAHTTGEGAQIEVAAVDAAAHWVPDGV
                     DAAANPDVCQARAGFLDETGRMRHWPRLAQYVTRGGEVLLLEAFARPTWEKFCHLVGR
                     ADLLELDRLGLDDATYHARLRQEIAAILIQRTRAEWMAAFIAADISAMPVNDPAALAR
                     DPHYLARGNWYEVDLPDVGPVRLNGTPIRLAGATFAPAPAPGLGEHGPAILGEVLGLA
                     DGEIAALRACGVVG"
     gene            complement(13698..15239)
                     /locus_tag="VFF98_07080"
     CDS             complement(13698..15239)
                     /locus_tag="VFF98_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZU63427.1"
                     /translation="MRHETLGAMLDFWARRWPDRLAVRTAARDASWGDLLRASDSIAA
                     GLAGLGVGAGDAVGILLHNRLEFIETMLAVWRLGARVTLLNIRLTESEMAYLTADARL
                     KVVVSQHELLGRLAVALAQQADLRLVLAEGGDGGADLAQWRAAGGQVAPAAAEAGDVA
                     LICYSSGTTGFPKGVMLSHRNLREGGLATAVPCGFTPDDRVLISAPLAYTWGAVQYLR
                     EALATGATAVLVDPAIGVEELLDVLTGERISLWSGVPILFERVAASPRFAGTAFPHLR
                     HAVSGGTSLDLLRQWQAKGVRLTQAYGLTETAGHVALLFGEHAEGRLGSAGRALLGVD
                     LAIADEEGGFLPAGHSGEILVRGAMVMQGYLNNPEETARAMRGDWLCTGDIGTIDAEG
                     FLTVTGRKKDMLRSGGLNIYPAELERVLAGIAGMAECAVIGVADETWGEVPMLIVHGE
                     GELDVEALRARCLTQLAGYKRPRYLLDYGAPLPRTFSGKIMKHALRAEFAVAPEQARR
                     LTYSA"
     gene            complement(15229..15756)
                     /locus_tag="VFF98_07085"
     CDS             complement(15229..15756)
                     /locus_tag="VFF98_07085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZU63428.1"
                     /translation="MRMQAPGNLFFDYDDPAVAAAPADGRAWAAVKGGGAFPDLAGPM
                     FCTGDALEAGEPARFGIRLMAHHCNAVAVAHGGLLASFADTALAQGLLCLGDVAFNTP
                     TITLNVDYLSASVLGEWLESRVVVAHRTLRMAFVSAMILAGARPVLRATGLFKTTPLA
                     DGGARTDRQETDDEA"
     gene            complement(15776..18106)
                     /locus_tag="VFF98_07090"
     CDS             complement(15776..18106)
                     /locus_tag="VFF98_07090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HZU63429.1"
                     /translation="MALLFALCAVGIGYGGIVLVALGGSAFFLIEAALLLGVAMLLWR
                     GNGWALWLHGALLGLVLAWSCREAGLDPWALVPRLGLPYLLGLLLLMPAIAGRLAGGP
                     GWPRGIRRAMRGLLAAALVGVPAAALLGHGYAEYGPPYQPPSAHGADASGAGADWADW
                     GGNGGGTHFSRLAEITPANVGQLQEAWRFSFGQRVKGGLEGTPLKIGDALYACDSTDQ
                     LVALDPETGRVQWRFDPHVRLDAPFRACRGIGYYRLPGRSGPCAERLYLATVDARLIA
                     VDAHGAGRCADFGQGGDVDALAGLGDVPRGYYWFNSAPNVFAGRIIVSGSMVDNQYWG
                     QPSGVVRAYDAVSGQLAWAYDAGAPERRGAPPAGQSYTRATPNAWAQMTFDPALGLLY
                     VATGNATPDYYGAQRRPFDDETSSAVLALDMATGRRRWLFQTTHHDLWDYDVGAQPVL
                     FDYAGADGRSVHALAQPTKRAEVFLLDRATGHPLGRIEERPAPTAGHAAGERVSPTQP
                     FPADFPSLAGPPLSEARMWGLTPFDQLWCRIQFRRARYDGALTPPGEQPGITYPGYLG
                     GMDWGGVSVDTDRAIMVAVSAYVPNYTRLVPRAQAGDVRPMSKGREGLAALEGLNAMA
                     GTPYASSTKPFLSPLQVPCNQPPWGRLSAIDLKTNRLIWSRPLGTGRDNGPLGIATRL
                     PLTIGAPVMGGALATRGGLTFVGASTDRSFRAFATASGRLLWQADLPHSGNAVPMSYL
                     SRASGRQFVVIAAGGHASMGAEQGDDLVAYALPRQH"
     gene            complement(18312..19124)
                     /locus_tag="VFF98_07095"
     CDS             complement(18312..19124)
                     /locus_tag="VFF98_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63430.1"
                     /translation="MSGAVGMAGPRSLAGKVVIVTGAASPRGIGFATARRLAAAGARL
                     VLTDLDGAAAAQRAAELGGMVLGAQALGAQALGLAHDVGREQGWADVLAASLARFGRI
                     DGLVNNAGIVQLAPVEAVTLADWHRQIEVNLTGAFLGCRAVIGQMRAQGGGGAIVNVS
                     SVAGLVGMRRTSAYAASKGGVRLMSKTLALETAGDGIRVNSVHPGVIETDIQQGVRAG
                     NPADSAAIATAIPLGHTGHPDHLAAAIAFLLSTDAAYVTGAELVVDGGLTAQ"
     gene            complement(19121..19444)
                     /locus_tag="VFF98_07100"
     CDS             complement(19121..19444)
                     /locus_tag="VFF98_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZU63431.1"
                     /translation="MAKLMVTLRSGECREVEWREGVSVKDVILDAGIFEINGITNCGG
                     CCSCGTCHVIFEPESAARLPAPEEGELDLLDIVPDRQGGSRLACQVPCSQALEGARLR
                     IGSGE"
     gene            complement(19498..20745)
                     /locus_tag="VFF98_07105"
     CDS             complement(19498..20745)
                     /locus_tag="VFF98_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU63432.1"
                     /translation="MDKALAQPAAAQAQAANDIDEALIVDWDIYTEPKLLEDVHLGWH
                     ALHDTAPDIFWTPRNGGHWVVMRYEDQVQVLKDAEHFSSRELHIPPMFNGNVMIPLNL
                     DPPEHTRYRAVLMRYFGPAAINRMEPKLVAWAHRLIDRVAADGACDFTEILGAGFPVS
                     VFMELMGMPLDRFEEFRGIVLEYFSNITPERRGQVQERIFAVMRELFDEKRRHPADDL
                     ASALLGEEVRGRPLTQEELESMGFLLFLGGLDTVANALTFSIRNLAGRPDLQQRLIAE
                     PEKLPDYVEESLRRFAVVNQTRIVKKDTVIKGAAFREGQMVMCPLTAAGLDPRRNPQP
                     ETFDIDRADRGHITFSIGAHTCIGNMLARLEMRVFTRVWLERIGAFALAGADRPAWRP
                     GLVMALEHLRLQWKAPGNAPAQA"
BASE COUNT         3662 a         7351 c         6734 g         3228 t
ORIGIN      
        1 ccgatgtagt ggatgggcgc ctgggtcatg cgagccgcgg gaaacgggca tcgtccagct
       61 cgcccagatg ggtgaggccg gcggtgtcga aagccaccga gggggcgcct gtccagcgcg
      121 catcggcagg gttcatgatc gccagatagg tccagcgcgg ggtgccggcg gtgttggccc
      181 cggccatgtg cacgcacagg tccgaatgca ccaccacatc ccccgccagc agcgccagct
      241 ggcccgagct ggggcaggca tcggccagtt cgggatagac atcgagcagg ttgccatcgc
      301 catagctgcg ataatccccc atcacgccat ggcggtgcga gccgctgaga taggccatcg
      361 tgccatgcgc cgcggtcatg tctgtcagcg gggtccagaa caccatgccg cccgagcgat
      421 cggccacctg cgcggcaaaa tcctggtgcc agtcagacct gccattgccc tcgccggggg
      481 ccagcggcag cttcacgcca aagaaatcga ggaaatagcg cgcccccacc ggccggcgca
      541 gcgcgcgcaa ggccaccgcc gcccgggcgt agtggcgcag caccggatcg aacaccggct
      601 ggcccacccc atgaatcggc aatgcattga aataggagaa cgcccccggt gccagatcgc
      661 ggtcggcatc ctcgcccatg cgggccctgg ccaccgctag catcgccgcc acatccgcgc
      721 ccggcaggaa cccgcgcagc cgcacccagc catgcgcctg ataatgcgcg atctcctctg
      781 cgctgacggc gcggtagggg ctggcaggcg cgcgcggttc tacaggttcg gtcacgccga
      841 tcgtcatctt tggccctttc agatgcggtc acatccacag gcgggccggc ctgctcctgc
      901 atgggcgcaa tcctgacctg ccactctccg gcgtcgattt ttatataacg ttgttataaa
      961 taaaactggc cttttcggca gggcccctca cgaagggggg caggatcgcc atcctgcccc
     1021 cgcggccggt tcagaaatgc acggtcagag cgccgccata ggtgcgcggc gcggccagca
     1081 tcagcacgct gtaataggga ttggcctggg ccagcgtgcg caccgcggta ttttccagat
     1141 tgcgcacgaa gaggcgcgcg ctccacacct gcccggccgg cgtatagctg aggaaggcgt
     1201 tgctgcgggt aaagccgggc tgccaggaat cgagcgtgcg ctccaccgtc acccaataac
     1261 cctgcgagac atgcgtatcg acatgggctg tcagcagcgc ctgattggcc aggtggaact
     1321 ggtgctcgta gttgaggtcg acggtcagcg cgggcgaatt gggcagggtc tggccgctgt
     1381 gatccaccgt gccggccagc gccgatgtgt agacaaaggc gccaaaccgg ctgtgcagat
     1441 aggcaaggct ggcgcccagc gtgtcattgg cggaaagccg cagccgacct tctgcctcca
     1501 gcccgtagat ctgggccgag gaggcgtttt gcgtcacgaa ggcgaacacg cccgagacga
     1561 tatcggcata gctcgcctga tagttgctgt agcgatagta gaaggccgag gcgttcagtt
     1621 gcagccggtt gccccagaag cggttcttgc tgccaatctc gaaggaagtg atcttttccg
     1681 gattgtaggt ggccaccggg aactgctgcc catagccgcc cgccttgaag cccgaggaga
     1741 cctgcgcata ggccatggaa gcggccgaca gatccgcctc cagctcggcc ttgtaggtca
     1801 gcgaggacca gcgggtggag gaataggcgg tgcccagcgc ggcattggca taatagaagc
     1861 gcgccgattt ttcatcgccg gtgtagcgca ggccccctgt caggcggatg gcatgggcaa
     1921 gcggcaccgt gacattggca aaggccgccc aggaactggt gccggcatcg cgcatttccg
     1981 gatcggttgc cgcctccagc gcgaaggaca ccggctctgc cgccgcctgg cgccgctcca
     2041 tgccgctcag ataatagagg cctgccaccc atttgatggc gctgcgatcg ggcgaggcgg
     2101 cgcgcagctc caccgaatcc tgccgccccg tgcggtgatc gcccacgccc ggcgtgccca
     2161 ccagcacatt ggcctcgtct ttggaaaaga agctgtgcga ggccagcagg gtgaggttgg
     2221 ccgcagcggt ggtgtaatcc acctgcccgc gcacattcca cgcatccacc cgcagatagc
     2281 cggtcggctt ggtgctgtac cacggcgcat ggcccgacag cggcgcatcc accgtgccca
     2341 ggccattgcc cgcattgtgc agatagtcgc ccgccagcac cacgcgcagc gcgctgcccg
     2401 gcttgtagag cagcttgagg cggcccgcct tcacatcggc atcatcattg ccgttcgaca
     2461 gatagccgct gtggcgttcc gaggcgaagg cgccgcgcag cgccaggctg ctgcccaaag
     2521 gcaggttgac cgccgcctcg gaatggatcg catcataatt gcccgcctgc agcgtggcat
     2581 agccgctggg cccggcgagg ctaggatcgg cggtgagcac gttgatcgcc ccggcattgg
     2641 cattgcgccc atacagcgtg ccctggggcc cgcgcagcac ttccacccgg tccacatcga
     2701 acatcaggct ggtgggcact tcggtttcct gctggtagac cccatcgatg ttgaggttga
     2761 ccgcggcatc gcccacttgg gaatcgccgt tggagccaac gccgcgcaca tagatctggg
     2821 cgccattggc attgccctgg atcaccaccg cgggcgtggc gctgattgcc gcggcaatgg
     2881 tgttcttcgc ttcatcgcgc aaggtggcgc cgctcagcgc ggtgatggcg atgggcgtgc
     2941 gctggatgtt ttcgctgcgg tgctgggcgg tcacgatgat ctcgcgcagg ccgccatcgc
     3001 cgggcggcgg cgcatcggcg gccggtgcat cggccatcgc cagcgccggc gcatcagcca
     3061 gcgccagcgc cggcgacgcc agcacgccca tcatccctgt tgcgcacaaa aatctggtcc
     3121 tcatggttct ctccctgtcc tctctggccc ggaaaggggc gccggccggc ggctccccgc
     3181 cccctgctca cgcagggggc agatgcggtg atgccgcgat gcaagcgggg gtgggcggct
     3241 tggcgccgcc tgcctggccc tgcctctccc acagttccgg ttggccaagg cttgcgccaa
     3301 acagcggccc gacgggaaac cgtttccatg gatggctcgc atcaacccga cgcggggcct
     3361 gcgcgcacgc cgcccttgct aggagaggcg ctggcgccgg ccgcctgccg gccgcccccc
     3421 aaggagagga tgcagatcat ggagcgctgc cgcttcgatg cgtatatcgc ccgtttcaac
     3481 gccaaggatg ccagcgcctt cgacgaatat ctcagccccg acatgcgcct gcgcaacggc
     3541 cggctggaat atacagggat agagggcatg aagcgccatt atgcggcgat ctgggcctgc
     3601 atggacgaaa cgctggacgt gctggattac gtgaccgatg gcgcccgcgc ggcggtggtg
     3661 atgcacacgc attttgccgt gcgcacggat gccgccacct cgcccttcgg cccgctgcgc
     3721 cagggcgagg ctttcgacta tcacggcgtg atcttctacg agatcacccg ggggcgcttc
     3781 tcgcggatca tcgtttccta tctcgatttc gtgaagacgg acacagatgg ccggcagacc
     3841 tcgctgggca tcgtgcactg aggggcccgg ggcgccagct tgtttccggc ctcagatctg
     3901 gccggcatag gcgcgcaggc gccccaccag ccacatcagc gccggatcat tgctgccgtt
     3961 cggcgtccac tgggcggtaa cgcggatgcc ggggatgtcc atcgggcatt ccagtatccg
     4021 cagcggcagc gaccgggcca tgcgctcggc cagcttgcgg tgcatggtgg cgatccgctg
     4081 cgtgccgaca aggaagctgg gcaccgcggt gaagctgggc gccaccactt ccacctgccg
     4141 cttcaccgac tggctcttga gcgcccattc ctcgaaagag ggcacccgcg tccagccaaa
     4201 gcgggtcacc acatggtgca tctcttcata ttcggcccgg ctgatgccct ggcgcagcgc
     4261 ccggttgccc gaccagcaca ccaccacgaa atcatccgtc cacagctcgg taaagggcat
     4321 gtcggtggaa atcacgctgt ccagcgccag cagcagatcg gccctgccgc gccgcaattc
     4381 ctccaccacc tcatcgccca gcgccttcac ctcgaagacc atgtgcggcg cctcttcctc
     4441 gaaggcggcc atcgccgggc ccagcatcac ctcgatggca taatcggacg ccagcacaga
     4501 cagccgccgg tccgacacgc ccggatcgaa gggctgcttc accgccatcg tcgcctggat
     4561 ctggtcgagg atggcgcgca ccggatcgag cagctcctgc ccgcgcgcgg tgagcaccag
     4621 attgcgcccc ttcagctcca gcaggtcatc ctcgaaatag tcgcgcaggc ggcccagcgc
     4681 gctgctggtg gcagactggc tgaggttcag ccgctccgat gccagccgca cgctttgctc
     4741 ttccagcagc gcgcgcaggg caatcaacag gttcagatcc agccgatgaa agcgcatgcc
     4801 cggttatccg ccgcctcatt ccccatgcgc aggggctgct gccctgcctc ttgttgccag
     4861 tgttgtgata gccgggccgc cgggggccgg caagccgccc tcaacccgcc tgttcctcga
     4921 cgaattcgat gaggttgccg gcgcaatcgc gaatgaagaa gcccttgccg aacgcctcgc
     4981 gcacgacaaa cacgatctcc acgccgcccg cctcgaactg cgccagcgcc gcctcgaaat
     5041 ccgccacccg ataggcgaca tgcttgttgc catgcacgcc cagatcgccc ggcacgctgc
     5101 ggcgctctgg cggcaggggg gcagcgccgg caatctcgaa cacttccacc cgcatgtcgt
     5161 ccttgcgcag catggccgcg cgcgaatggg ccggcggcac cgaaaaacgc ttctccagcg
     5221 tgtagcccaa cgtctcgcca taccaggcga tggcgcgatc gagatccggc acgctgatgg
     5281 cgccgtgatg aaaacgaaat tgcggcatcg gcttctctcc tgcctgtgcg cgacaggtaa
     5341 accccggcga tggggccgcg cctgtcgcac gcgcggccct ttcctggggc aagggggatc
     5401 aggcggcggc cggcttgcgc tgccaggcca gcgtggccag cgccaccacc gcgcccgcgc
     5461 ccgcacccac cgccaggcag ccaaaggcgg caaacagccc gccaaagcgg ttggccagat
     5521 aggtcaccac caccggacaa aggaactggc ccaccgccag cgccccctgc cacaggcccg
     5581 agccgcgccc gcgcacctcg aaggccagca gccccatcgc ccacaccagc aacgtgggca
     5641 gcagcatgcc ggcgcccagc tggttgatga agcagccgac gaggaaaggc gtgggcccct
     5701 gcgcccgggc catgacgaga aagcccgtgc acagcatcac catctcgatc agcagcagcc
     5761 agcgcggatc cttgaggccg cttctggaat agaccaccgt gcccagcggc acgcccacgc
     5821 tggcgatgga agtgaggaag ccgatccgcg ccggatccgt gaggcccagc tgggccaggc
     5881 ccacgctggc ctggatctgc acggtgtaga agaacaccga gctgtagatc gtgaccagca
     5941 gcaccggcac gaagcgcgcc agcggaaaat gcgcccagct ggcatgatgg ggggcatggt
     6001 cggcctcgct ggcggcattt tcctcggccg gctcccaggt gaagcgcagc accatggcaa
     6061 acatcgccag cgccgacaga tagatccaga acggcgtgcg ccagccaaac gcccccagct
     6121 gcccgccgat gttgaagaac agcagcgccg aaagcgaggc ggtggcagcc tgcgcggcca
     6181 gccatttatc ccgcgcgggg ccgtcgaaat aatcgccgat catcgtcgtc gtcagcacca
     6241 tgatcagcgc ctcgaacacc cccaccagca cgcgcgaggc caggatggcc ggcaggctga
     6301 caaggaagag cggcgcgatg cccaccaccg catagcccag gaacgagcac agcagcaggc
     6361 gccgccggcc gaaataatcg cccagcatgc ccgccaccgg cgagagcagg gcaatgcaga
     6421 tcgccgggaa ggtgagcacc agcggcaccc agtaatcggc atggggcccc tggcggaatt
     6481 cttccatcat gcgcggcaga atgggcgcca gcagcacgat cgccatggtg gaaagggtga
     6541 tgggtatcag cagcgacagg cccgtgagga accccggtct gtgggtgcgg gcgcggttgt
     6601 gagcggcggc catgcttgtg cattctccga aaaagggcgc gcgcctgtcc gctgccgccc
     6661 ctgtggcagg ggccgcccgc gccggtcgcg tccggtgtgt tgtgtgcctg tggtggccgg
     6721 gcgcctcagc ggcggcgcca gctcatccgc tgccagctca ttcgctggag gaagggctgg
     6781 cgatccaccc actggtgctt cagcgcgccg gcaatgtgca gcgccagcag cgcgatcatc
     6841 gcccaaccgc ccgcctcgtg ccaatcaacg aaactgtcgt gcaggcgctt ttgcgcgggc
     6901 agcccgccgg gcgtatcgcc caccgcctgc acaaggccga tcgccggcac cgggatcacc
     6961 caccaggcgg tgatgcccgg ctttggccgc agccggtgca ccgcaggccc ggcctgcgcc
     7021 gccatgcgcg cctgctcggc cgcaaagccc tgactgcccg gggccggatg ggccgacagg
     7081 atcgcccagc cggtgagcgg cagcaccacc atgccggcat agagcaggaa atggacgata
     7141 tgcgccgccc cctgctccag ccggccaacc ggggccggcg gcggcacatg cagcaaccgc
     7201 cagacgatcc gcaccacgct gagcgccagc acgctgagcc cgcaggagat atgcagcggc
     7261 agcaccacgc ccttgagcgg cggcgcaaag ccttccatga accagcccag cgtgaggttg
     7321 cagccgatca tcagggcgat ggcccaatgc agcagcacag ccatgcgcgt gtagcggatc
     7381 gcgtcagcag atgtggtcat gcccactcct catccgacaa ggccttatcc ggcctgttct
     7441 cacccggcca gcgcgccggc aggcttgccc gctgccggca cgcgcccgcc tcaataggcc
     7501 tcaacctggc tgaaggtgca atagggcttg ggaatggccg ggttgtattg cggatcgcgg
     7561 ctggtggtat agccgggata gaagatcttc ttctcgtcgg gcttcacgcc atagaaggtc
     7621 gatccggcct cttcctgcac cccggcgcgc agcgagccat ccggattctt gccttccagc
     7681 gccagcgtgt cgacagcggc cagattgttg cgcgaatccg tccacagctt gttcacatcg
     7741 acatagaatt cgacataggt gcgcaagtgc cagatcttcc agtggccgtc ctccttgatg
     7801 aagtccatgc cgtatttctc ttccgtccac tgcgacatcg gctggccggc atgcatctcg
     7861 cccgagacat tgccgaagga catgaagatg cccttggcgg ttttgccatc ggcggccact
     7921 tcgatgtagg gggtggtcag catgtgatac cacaaggcgc ccttgtgcgg gaagttcttg
     7981 aggttatcga cgtagaactt ccacagcgag gtgcgattgg cgtatttgtc ggtgccgttc
     8041 gtccaggtga tgtcctcgcg cttcgaccag gcattctcga tctcggcctg atgcatcagc
     8101 gcgccgtggt aatattcgtg gctggacgac acattggtga tctcggcaat atccgacagg
     8161 cgctggatct gtttggtcgg cgttatcggc ttgccgcaag cgtctgtgcc ggcagcccgg
     8221 gccccacctg ccagcaccac cgccgacagc aaagaggcgc ccagtagcat cagctttttc
     8281 atcattctct cccttgcttt cgctctgcgg ccttatgcag aagcttggcg ggcaagggca
     8341 tggccgtcgt gtggatggct cgcatcgaca gggcggatgg gtgacgggcc ctgcctgcag
     8401 gcccacaggc gaaaggcgcc accagccgga gggaggaccg gccagcagcg cctttcctcg
     8461 ggagcaaggg cgcagcctcc cccccgatgg acaggatcag taacgatagg tcagcgaacc
     8521 gccgatcgtg cgcggcgccc cccacacctg ccggctggcg aaagtgtcgg tgaaattggc
     8581 gatgcgatac atctggttgg tgacattgtt ggcatagacc gacagatcca gcttcttcga
     8641 cggatccgtc catgtcgcgc gcaggttgag cagcgtgtag gaggtttgcg gcagctgcat
     8701 cgcctggtcg aaatagaaac catcggaata gaacaggttg ccattcagca ccaaccgccc
     8761 cttgggcaga tcaaagccat aatccgcgcc gatcgatccg gcaaagcgcg gcgtgttctg
     8821 caccggatag ccggtggcat tcacattata gggctcgaac agcgaatagg tggcgctgct
     8881 ggtgttcaga tcctgctgat agccgatggc attctcgaac cggccataaa cggcatgggt
     8941 gtaggcgccg ctgagcgtaa cgtggaaatc gggcgtgatg gcataggaga gatcgccatc
     9001 gacaccatag atatgctccg aagcggcatt gcgcaccacg ctgacgcccg aggcaaagaa
     9061 cgaggccacc tgcacatcgc ggtaattgta gtaataggtg gccaggttga agcgcagcgg
     9121 cccgctggcc accttgtagc ccagttcgaa cgcatcaatc ttttcaggct tcacaggcgt
     9181 gaaggaaaag gccgagcccg gcagcgcgcc cgatttatag ccgcgggtat aggatgcata
     9241 gatgttcgac ttgggcgtca gctggtagcg gatcacgccg cgcggcgaga aattgttgaa
     9301 ggaggtgctg cccgaggcag tggtgcccag caggttgaac cacaggccaa ggtcatcctt
     9361 gctgtagcgc ccgccggcgg tgaggaagag cttgtcgagc agctggtagg tcacatcggc
     9421 gaagacggca taggaattgg tggtgttctt cgatgtgaac aggtggaaat agggcgcgcc
     9481 ggccgctgtc gtcgccagat tgtagtcgta gacgtcggag gcccagtaat agaaggcgcc
     9541 cagcacccac gacaggcgcc cgccgggctt ggaagtcagg ttgaattcct gggtgaagct
     9601 cttgtcgggg atggtccagg cggccgcgtc gatggcggcg gtggtgtcgt catattccag
     9661 cgcctgggcg acatagtcct tgcgatagcc ggtataggat gtgagatccg cccagctcat
     9721 cttcagcacg ctggtcagcg tgacggcatc ggtgatgatc ttgttcgcat tggtggcgcc
     9781 cggcgagatc tggttgggcg cggtggcgat ggtgaggccg ggaacgacat tgccggcggt
     9841 catcggcttg ccgctgccat ccacgcgcac attggtgctg ttgagcagcg gatcgtcgga
     9901 ataggtgtgg gcatagccca gcgtgaagct gagattgctg gtgggtgtcc acagcagctt
     9961 ggggcgcacg gtccatttgc gatattcgcc ggcgtggtcg ttgccgttga ggatgttggt
    10021 gatgtagccg ttgctgtgtt cgtaatcggc ggcaaggctg atggccagcg tatcggtgag
    10081 gccggtggtg ccatagaagg ccgcggtcga atggctgaac gattcgtagc ccagcttgaa
    10141 ggtggcctgc gtctcgtgct gcggcgcgcg ggtggtgatc tggatcgcgc cgcccgtggc
    10201 attgcggccg aacagcgtgc cctgcgggcc cttcagcacg ctcacgctgt ccacatcgat
    10261 caggtcgatg tcggaagagg tggggctgga gatgtaatag ccatccacat agaccgggat
    10321 gttcgaatag aggcccgggc ccgccaccga actgccgatc ccgcgaatgg agggctgcac
    10381 ggtgttgccg gaatagttga ggcgcagcgc cggcaccgat tgctgcagct gggccacttc
    10441 ggtgatgccc gacttggcca gctgcgggcc gctgaccgcg gcgatcgaga tcggcacgtt
    10501 ctgcagcttt tcagaccgct tctgcgcggt gacgatgatt tcctgattgc tgccggccgg
    10561 ggcgggcgtg gccgcgccgg ttgccgcggg ctggttctgc gcctgcgcgc tgccgccaaa
    10621 cgcaaccgca gccaggccca cacccagctg cagcgcaagg cgggtgcccg caatggaacg
    10681 agacaaattc gtgtagacgg ccatggcccc ctctcccaaa tgtgagcgat aatcgctcat
    10741 ccgttcgttg ttagcccaaa tctatatacc tccgccgaag cgtgtcaatc acgctgcgtg
    10801 ccctgcggcg gcatttttgc gcgaaagccc cgatccgtgt cggccatgcc accgcgccat
    10861 tgcggccagc gggtgggcgc gcgcggtctg ccggcgcagg cgcgcctgcc ccacgcctcg
    10921 tcaggcccgc cggcaaagcg ggctggaagg cagcgccatc agcgctgcag ctccagatag
    10981 acattcttgt ggcgcaggaa ttcagcgagg ccctcgcgcc cgccttcccg accgaacccg
    11041 ctttgcttgt agccgccgaa aggagcgggc gcgggcagcg gtgcaaagcc gttgatgctg
    11101 atgtagcccg cctccagccg cgcggccacg cgatgcgcgc gttccaggtc ggtggtgtgg
    11161 atatagccgg ccaggccaaa aggcgtgtca ttggccagcg ccaggccctg cgcctcgccg
    11221 ctgaagcgga tgatcgacag caccgggccg aagatctcct cctgcgccag cgggctgcgg
    11281 ttatcgacat cgccgaacac ggtgggcgcc atgaaatagc cctgcgccag ttcgccgccc
    11341 agccgatggc cgccagccag cagccgccca tggccctgtt gctgcgcccg ggccaccacc
    11401 gccagcaccc gctccagcgc cgccgcgttg atcagcggcc cataggccac acccggcgtg
    11461 cacgggtgcc ccacccgcgc ggccctgacg atcgccagca ccttctcgac aaagacatca
    11521 tagaccgcct cttccaccag caggcgggtg ggcagcacgc agccctgccc ggcatgggtg
    11581 aggcagccca tccgcgccgc catcccggcg gcccggtcaa gatcggcatc ggcaaacacg
    11641 agattggccg atttgccccc cagttccgat accaccggcg ttgcatgcac ggctgccgcc
    11701 gccatcacct tgcgcgcggt aggcaagccg ccggtgaagg aaatcttgcc aatgcgcgga
    11761 tcggccacca gggcggcccc cgcctccgcc agcccggtga cgacattgag cacgcccggc
    11821 ggcaggcccg cctccagcat cacctcgccc agcaaaagcg tggcgaaggg ccccagatcg
    11881 ggcgccttca gcaccacgca attgcccgcg gccagaatcg cgcccagctt catcagcgcc
    11941 gtcgccagcg gcccgttcca ggcattgatc gcggccacca cgccataggg ctccagcagg
    12001 ccataattga aggcgcccgg ccccagggcc accgtgctgc cctcgatctt gtcggcccag
    12061 ccggcgtaat agacgaggtg ttcggcggcc tggccggcgg cctgcggggc atagacagcg
    12121 ccgctttccc gcgcggcaat ggcggcgaat tgggccccgc gcgcgcggat cgcggcggcc
    12181 gcgcgcagca gcacatcgcg gcggaaggcg ggctgtgcat cgcgccaggc cggaaaggcc
    12241 cgctggcacg aggcgatcgc ccgatcgata tccaccgccc ctgccagcgc gaatgcgccc
    12301 agcggcaggc ccgtggaggg atcgtgatgc agctggctgc ggccgctggc ggcggccacc
    12361 cattcgccat cgatcaacat cgccgcccgc gcgaaaacgg gcgagatatc ggccatggcg
    12421 gcttgctggc aggggtcctg cgccatcgct caacccacca cgccgcaggc gcgcagggcc
    12481 gcgatctcgc cgtctgccag gcccagcacc tcgcccagga tcgctgggcc atgctcgccc
    12541 agcccgggcg cgggcgcggg cgcgaaggtg gcccccgcca ggcggatcgg cgtgccgttc
    12601 agccgcaccg ggcccacatc cggcagatcc acctcatacc agttgccgcg ggcaagataa
    12661 tgcggatcgc gggcaagggc ggccggatcg ttcaccggca tggccgagat atcggcggcg
    12721 atgaaggcgg ccatccattc cgcccgcgtg cgctggatga ggatggcggc gatctcctgg
    12781 cgcaggcggg catggtaggt ggcatcatcc agcccgagcc ggtccagctc cagcagatcc
    12841 gcgcgcccga caaggtggca gaatttctcc caggtcggcc gggcaaaggc ctccagcagc
    12901 agcacctcgc cgccgcgcgt gacatattgg gccaggcgcg gccaatggcg catccgcccg
    12961 gtctcatcga gaaagcccgc gcgcgcctgg cagacatccg gattggccgc cgcatccacg
    13021 ccatcgggca cccaatgggc cgccgcatcc accgcggcca cctcgatctg cgcgccctct
    13081 ccggtggtgt gggcgcgcag cagcgcggcc agcaccccca ccgcggcaaa caggccgggc
    13141 gcctgcatgc cgatggacgg cggccggaag gcatgggcgg cgggcggcgg gctgtccaca
    13201 tcggcaggca ccaggccggc ataggcatca taggccaccg catgggaacg cagcgcatga
    13261 tagggcccgg tgcgccccag ccccgaaatc gcgcagaaca ccagcgccgg ccgccgctgc
    13321 cgcaaaacct cgtagcccag ccccaaccgc tcgagcgcgc cgcccttcag cccctcgatc
    13381 accacatcgg cccggctggc cagatcgcgg aacagcgcct gcccctgggg tgttttcaga
    13441 tcgaggccca ggctctgctt gccccggttc cagcgcagat gcagcagccc cacgccatcg
    13501 ggcccgccca gcgcatgttc gcccgtgaag cgcaccggat cgccgccggg caaggcctcc
    13561 accttgatca cccgcgcgcc catgtcggcc agatggccgc ccagcgcatc catgcccagc
    13621 tgcgccactt ccagcacggt gacagtcttc aggaaatcat agccagccat cggccttctc
    13681 cgcaaaatga agggggctca ggcgctgtag gtgaggcgcc gggcctgttc gggggccacg
    13741 gcaaattcgg cccgcagcgc atgtttcatg atcttgccgg aaaaggtgcg cggcagcggc
    13801 gcgccataat ccagcaggta gcgcgggcgc ttgtagccgg ccagctgcgt caggcagcgc
    13861 gcgcgcaagg cctccacatc cagttccccc tcgccatgca cgatcagcat cggcacctcg
    13921 ccccaggtct cgtcggccac gccgatcacc gcgcattcgg ccatgccggc aatgcccgcc
    13981 agcacgcgct ccagttcggc cggatagatg ttgagcccgc ccgagcgcag catgtccttc
    14041 ttgcggccgg tcacggtgag gaagccctcg gcatcgatcg tgccgatatc gccggtgcac
    14101 agccaatcgc cccgcatggc ccgcgcggtt tcctccggat tgttaagata gccctgcatc
    14161 accatggccc cgcgcacgag aatctcgccg ctgtgcccgg cgggcagaaa gccgccctcc
    14221 tcatcggcaa tggccagatc cacgcccagc agcgcccgcc cggccgaacc cagccgcccc
    14281 tcggcatgtt cgccgaacag cagcgccaca tggccggccg tctcggtcag gccataggcc
    14341 tgggtcagac gcaccccctt ggcctgccac tggcgcagca gatccagcga tgtgccgccg
    14401 ctgacggcat ggcgcagatg gggaaaggcg gtgccggcaa aacgggggct ggccgccacc
    14461 cgctcgaaca ggatcggcac gcccgaccac aggctgatcc gctcgcccgt cagcacatcg
    14521 agcagctctt ccaccccgat cgccggatcg accagcacgg ccgtggcgcc cgtagccagc
    14581 gcctcgcgca gatattgcac cgcgccccat gtgtaggcca gcggcgcgga aatcagcacc
    14641 cggtcgtcgg gcgtgaaacc gcagggcacc gccgttgcca gccccccttc gcgcaggttg
    14701 cggtggctga gcataacccc cttgggaaag cccgtggtgc ccgaggaata gcagatcagc
    14761 gccacatccc cggcctctgc cgcggccggc gccacctgcc cgcccgctgc ccgccattgc
    14821 gccagatccg cgcccccatc gccgccctcg gccagcacca ggcgcaaatc cgcctgctgc
    14881 gccagggcca cagccagccg gcccagcagc tcgtgctggc tcaccaccac cttcagccgc
    14941 gcatcggctg tcagataggc catttcgctt tcggtgaggc ggatgttgag caaggtgacg
    15001 cgcgcgccca gccgccacac cgccagcatc gtctcgatga attccagccg gttgtgcaaa
    15061 aggatgccca ccgcatcgcc cgcacccacg ccaaggccgg ccagcccggc cgcgatgctg
    15121 tcgctggccc gcagcagatc gccccagctg gcatcgcgcg ccgccgtgcg cacggccagc
    15181 cgatccggcc agcgccgggc ccagaaatcg agcatggcgc ccagggtttc atgcctcatc
    15241 atcggtctcc tgcctatcgg tccgggcgcc cccgtctgcc agcggcgttg tcttgaacag
    15301 gccggttgcc cgcagcaccg gccgcgcgcc ggccaggatc atcgcgctga caaaagccat
    15361 gcggagggtg cgatgggcca ccaccacccg gctttccagc cattcaccca gaacagacgc
    15421 cgacagataa tccacattga gagtgatcgt cggcgtgttg aaggcgacat cgcccaggca
    15481 cagcaggccc tgtgccagcg cggtatcggc aaagctggcc agcaggccgc catgggcaac
    15541 cgccaccgca ttgcaatgat gcgccatcag ccggatgccg aaacgggccg gctcgccagc
    15601 ctccagcgca tcgccggtgc agaacatcgg cccggccaga tcgggaaagg cgccgccgcc
    15661 tttcaccgcc gcccatgcgc ggccatctgc cggcgcggcg gccacggcag ggtcgtcgta
    15721 atcgaaaaac agattgccgg gggcctgcat gcgcatgcct cactctcccc aaatgtcagt
    15781 gctggcgcgg cagcgcatag gccaccagat catcgccctg ttccgccccc atcgaggcat
    15841 ggccgccggc cgcgatcacc acgaactgcc gcccgctggc gcgcgacagg tagctcatcg
    15901 gcacggcatt gcccgaatgc ggcagatcgg cctgccacag cagccggccg ctggccgtgg
    15961 caaaggcccg aaagctgcga tcggtgctgg cgcccacaaa agtgagcccg ccccgcgtgg
    16021 ccagcgcgcc gcccatcacc ggcgcgccga tggtcagcgg caggcgggtg gcgatgccaa
    16081 gggggccatt atcccgcccg gtgcccagcg ggcgcgacca gatcagccgg ttcgtcttca
    16141 gatcgatcgc gctcagccgg ccccagggcg gctggttgca cggcacctgc aagggcgaga
    16201 ggaacggctt cgtgctcgag gcatagggcg tgccggccat ggcgttcagc ccttccagcg
    16261 cggccagccc ttcgcgcccc ttgctcatgg gccgcacatc gcccgcctgg gcgcgaggca
    16321 ccagcctggt gtaattgggc acataggcgc tgacggccac catgatcgcc cggtcggtat
    16381 ccaccgacac gccgccccaa tccatgccgc ccagatagcc cggataggta atgcccggct
    16441 gctcgcccgg cggggtcagc gcgccatcat agcgggcgcg gcggaactgg atgcggcacc
    16501 acagctgatc gaagggggtg agcccccaca tccgcgcctc actgagcggc ggcccggcca
    16561 gcgaggggaa atcggccgga aaaggctggg tgggcgatac ccgctcgcct gcggcatggc
    16621 cggccgtggg cgccgggcgc tcttctatcc gccccagcgg gtggccggtg gcccgatcga
    16681 gcaggaacac ttcggcgcgc ttggtcggct gggccagggc gtgcacgctg cggccatcgg
    16741 cgccggcata atcgaacagg accggctgcg cgccgacatc ataatcccac aggtcgtgat
    16801 gggtcgtctg gaacagccag cggcggcggc cggtggccat gtccagcgcc aacaccgcgc
    16861 tcgatgtctc atcgtcgaag gggcggcgct gggcgccata ataatcgggc gtggcattgc
    16921 cggtggccac atagagcaag cccagcgccg gatcgaacgt catctgcgcc caggcattgg
    16981 gggtggcgcg ggtgtagctt tgcccggcgg gcggggcccc gcggcgttcg ggggcgccgg
    17041 catcataggc ccaggccagt tgcccgctca ccgcatcata ggcgcgcacc acgcccgaag
    17101 gctggcccca atactggttg tcgaccatgg agccgctgac gatgatcctg ccggcaaaca
    17161 cgttgggcgc ggaattgaac cagtagtagc ccctgggcac atcgcccagc cccgccagcg
    17221 catcgacatc gccgccctgg ccgaaatccg cgcagcgccc ggcgccatgc gcatcgacag
    17281 cgatcagccg ggcatcgacg gtggccagat agagccgttc ggcacagggg ccagagcgcc
    17341 ccggcaagcg ataatagccg atcccccggc aggcgcgaaa aggcgcgtcg agccgcacat
    17401 gcggatcgaa gcgccattgc acgcggcccg tctcgggatc gagggcgacc agctgatcgg
    17461 tcgaatcgca ggcatacagc gcatcgccga tcttgagcgg cgtgccttcc agcccgccct
    17521 tcacacgctg gccaaagctg aagcgccagg cctcctgcag ctggccgaca ttggccggcg
    17581 ttatctctgc caggcgcgag aaatgggtgc cgccgccatt gccgccccaa tcggcccaat
    17641 cggcacctgc accgctggca tcggcgccat gcgcagaagg aggctgatag ggcgggccat
    17701 attccgcata gccatggcca agcagcgcgg ccgccggcac gcccaccagc gccgcggcca
    17761 gcagcccgcg catcgcccgc cggatgccgc gcggccagcc ggggccacct gccagccggc
    17821 cggcgatggc cggcatcagc aacagcagcc caagcagata gggcaggcca aggcgcggca
    17881 ccagcgccca gggatccagc ccggcttccc ggcagctcca cgccagcacc agccccagca
    17941 gggcgccatg cagccacagc gcccaaccat tgccccgcca cagcagcatc gccacgccaa
    18001 gcagcagcgc cgcctcgatc aggaagaagg ctgagccgcc cagcgccacc agcacgatgc
    18061 cgccatagcc gatacccacc gcgcacaagg cgaagagcag cgccacacac cttgcccgga
    18121 tcaggccaga tgcattgctg gccgcagggg cgcccggata tccatccgcc atgcccgatt
    18181 tctcccattt gcgcctgtgt ccgcgcttaa tcaatcatcg attggtttaa tgcctgccct
    18241 gtccttgtca agccgccgca gcgcggtttg atgaattatc gtccccttgt gccttaatcg
    18301 accatttgcg cctattgcgc cgtcagcccg ccatccacca ccagttcggc cccggtgaca
    18361 taagccgcat ctgtcgacag caggaaggcg atcgccgccg ccagatgatc gggatggccg
    18421 gtgtggccca gcggaatggc cgtggcgata gcggccgaat cagccgggtt gccggcgcgc
    18481 acgccctgct ggatatccgt ctcgatcacg ccgggatgca cggaattgac gcgaatgcca
    18541 tcgcccgccg tttccagcgc cagggtcttg ctcatcagcc gcacgccgcc cttgctggcg
    18601 gcataggcgc tggtgcggcg catccccacc agcccggcca ccgacgacac attgacgatc
    18661 gcgccgcccc cgccctgcgc gcgcatctgg ccgatcaccg cccggcagcc caggaaggcc
    18721 ccggtgagat tgacctcgat ctgccggtgc cagtcggcca gcgtcactgc ctccaccggc
    18781 gccagctgca cgatgccagc gttgttgacc agcccgtcga tgcgcccgaa gcgggccagg
    18841 cttgccgcca gcacatcggc ccagccctgt tcccggccca catcatgggc aagacccagt
    18901 gcctgagcgc ccagtgcctg agcacccagt accatgccgc ccagttccgc cgcccgctgc
    18961 gccgcggccg cgccatcaag atcggtcaat accagccggg cccctgccgc cgccagccgc
    19021 cgcgccgtgg caaagccaat gccccgcggc gaagccgcgc ccgtgacgat caccaccttg
    19081 ccggcaagag agcgggggcc ggccatgccc accgccccgc tcattcgccg ctgccaatgc
    19141 gcagccgcgc gccctccagc gcctggctgc aaggcacctg gcaggccaga cgcgagcccc
    19201 cctgccgatc gggcacgata tcgagcaggt ccagctcgcc ctcttccggc gcgggcaggc
    19261 gcgcggcgct ctccggctcg aaaatcacat ggcaggtgcc gcaggaacag cagcccccgc
    19321 aattggtgat gccgttaatc tcgaaaatgc cggcgtccag gatcacatcc ttcacgctga
    19381 caccttccct ccactccacc tcacggcatt cgccggaacg caaggtgacc atcagttttg
    19441 ccatcgcctg ctctccctcc ccttcgtcgc gcaatcgtca gatccgcgcc cttttggcta
    19501 agcctgtgcc ggcgcattcc ccggcgcctt ccattgcagc cgcaggtgtt ccagcgccat
    19561 caccagcccc ggccgccagg cgggccggtc tgcgccggca agcgcgaaag cgccgatccg
    19621 ctccagccac acgcgggtga acacgcgcat ttccagccgc gccagcatgt tgccgatgca
    19681 ggtgtgggcg ccgatcgaga aggtgatatg gccgcgatcg gccctgtcga tatcgaaggt
    19741 ttccggctgg gggttgcggc gcggatcgag cccggcagcg gtcagcgggc acatcaccat
    19801 ctgcccctcg cggaaagccg cgcccttgat caccgtgtcc ttcttcacga tccgcgtctg
    19861 gttgacgaca gcgaagcggc gcagggattc ttccacataa tcgggcagtt tttcgggctc
    19921 ggcgatcagg cgctgctgca gatcggggcg cccggcaagg ttgcggatcg aaaaggtgag
    19981 cgcattggcc accgtgtcga gcccgcccag gaacagcagg aagcccatgg attccagctc
    20041 ctcctgcgtc agcgggcggc cgcgcacttc ctcgcccagc agggcgctgg ccaggtcgtc
    20101 ggcgggatgg cggcgcttct cgtcgaacag ctcgcgcatc acggcaaaga tccgttcctg
    20161 cacctgcccg cgccgctccg gcgtgatgtt gctgaagtat tccagcacga tgccgcgaaa
    20221 ttcctcgaac ctgtcgagcg gcatgcccat cagttccatg aacaccgaca ccggaaaacc
    20281 cgcacccagg atttcggtga aatcgcaggc cccgtcggct gccacacggt cgatcaggcg
    20341 atgcgcccag gccaccagct tgggctccat ccggttgatc gccgccggcc cgaaatagcg
    20401 catcaacacc gcgcgatagc gggtatgctc gggcggatcg aggttcagcg ggatcatcac
    20461 attgccgttg aacatcggcg ggatgtgcag ttcgcggctg gagaaatgct cggcatcctt
    20521 gagcacctgc acctggtcct catagcgcat caccacccaa tggccgccat tgcgcggggt
    20581 ccagaagatg tcgggcgcgg tgtcatgcag cgcatgccag cccagatgca catcctccag
    20641 cagcttcggt tcggtgtaga tatcccagtc gacgatcagc gcctcgtcga tgtcgttggc
    20701 tgcctgtgct tgcgccgcag ccggttgcgc cagtgccttg tccatttgca tcctcctcgg
    20761 gccggtttgc gcagcagctg tgtgccgcct gtgcgctgcc gggcgagcct tgcatggcct
    20821 gcccggcaga cgggaaccgc tgcgaggaga tatagaacag aacattgttc acattccatt
    20881 cgcgttatgc cacaccacgg catgagcacc ggcgccaccc ttcccccccc ggccgagaag
    20941 gccccccaca acgcgctgca gcgccgccgc ctgcc
//
LOCUS       DASRYE010000127         8494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_236531,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000127 DASRYE010000000
VERSION     DASRYE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236531"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..912)
                     /locus_tag="VFF98_07110"
     CDS             complement(61..912)
                     /locus_tag="VFF98_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63433.1"
                     /translation="MSTICILGLGYTAGEIAAGLRARGWQVEATGRAGTVDFADAERL
                     SWLLARCDAVLSSVPPVAGAGAAEDPALAAYGADLAALRARGGWLGYLSSTGVYGHCA
                     GAWVDETAPVASGRRHARMAADAGWLAAGAHVFRLPGIYGPDRPGEAPRNMFARLEQG
                     RAHRVAAPDQLFSRVHVADIASGVIAALERQAPPGAYNLADDAPCHPDLVVQEACRLS
                     GWPLPPRLAPDDPALSAKVRGFYGESRKVANGKAKRLLGWRPRYPSWREGLAAIWAGR
                     SAAGDRR"
     gene            971..3601
                     /gene="pepN"
                     /locus_tag="VFF98_07115"
     CDS             971..3601
                     /gene="pepN"
                     /locus_tag="VFF98_07115"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZU63434.1"
                     /translation="MTDARLQETPPAPPVIRREEYRVPDWLVPEVALAFALGLEKTRV
                     FATLSVRRNPEGTGNQTIRLHGDGLTPLLVEVDEVVRNDWRMEGADLLIELADDTHEI
                     TIETEINPAANSQLMGLYASGGMLCTQCEAEGFRRITFFPDRPDVLSTYRVRMEGDRE
                     TFPVLLSNGNQVAEGVFEDGRHWAEWHDPWPKPSYLFALVAGRLVANTGHFTTMEGRE
                     VTLNIWTRAGTEGEETRTDHALASLINAMKWDEEAFGRAYDLDLFNIVAVSDFNMGAM
                     ENKGLNVFNTRYVLADPDTATDGDFDAVEGVIGHEYFHNWSGNRITCRDWFQLSLKEG
                     FTVLRDQMFSADMGSPAVKRIEDVRILRSAQFPEDSGPLAHPIRPDSYQEISNFYTAT
                     VYNKGAEVIRMMRSMAGAERFRAGTDLYFSRHDGEAATCEDFVRAIEEGAGLDLTRFR
                     LWYAQAGTPELTARLDHAGDTVTLHLTQSVPATPGQLVKQPMPVPLRIALFNPQTGQH
                     GGEQLIVLDSEEASFAFQGFASRPVLSINRGFTAPVKLATDHRPEDLTFLAAHDDDPF
                     ARYEALQKLIADVLCGLVKGQEQAGGADRGAGYAAIAGAMRAIIADPALDDLMRGELM
                     ILPAHAYLAELLELVDPLALHAEREALKAHLGRTLAAELEALHDRASAVPFSLAAGAK
                     GARKVKTQALVYLAAGQPEAAAARAAAQYDAATNMTDRQGALMVLCGLDTPLRAQKLA
                     HFHARYAGNALVIDKWFSLQAGSLHKDVLAHVQALAGHPDFTLHNPNRVRALYMAMAV
                     NPAAFHAASGEGYRMIADLILRLDPINAQTAARFVPSLGRWRRMAGGRSALMKAELER
                     IAQVPGLSKDVFEQVSRSLG"
     gene            3594..4373
                     /gene="pgeF"
                     /locus_tag="VFF98_07120"
     CDS             3594..4373
                     /gene="pgeF"
                     /locus_tag="VFF98_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZU63435.1"
                     /translation="MAEASQPEVLRSPALAGLRHGFLGRRGGVSTGLVAGLNVGAGAI
                     GPDGDGAAQVAENRARAVAAVAPGARLVTCYQVHSALCVEAGDWAEAARPHADALVTR
                     QPGVVLGVLTADCAPVLFADGEAGVVGAAHAGWKGAFGGVCEATIAAMEQLGAVRGRI
                     AAAIGPCIAQPSYEVDDGFRARFCAEAPAQAAFFAAGRAGHWQFALEAYVASRLAAAG
                     IGRIDLLGQDTYADADRFFSFRRATHRQEPVYGRQISLIGL"
     gene            4641..5222
                     /gene="petA"
                     /locus_tag="VFF98_07125"
     CDS             4641..5222
                     /gene="petA"
                     /locus_tag="VFF98_07125"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZU63436.1"
                     /translation="MTENAIADTPPGANAGAGAEDGVRRRDFIHIAALSAAGVGGAAT
                     LVPLVSQMAPSADVLAESSTEIDISQIKPGQAIKAVFRKQPVFVRNLTPAEIAAANAV
                     PTASLRDPETLAQRTKAGHTNWLITMGVCTHLGCVPLGTGQGEPKGEFGGYFCPCHGS
                     QYDTAGRIRKGPAPKNLQVPEYAFSSATVVKIG"
     gene            5234..6514
                     /locus_tag="VFF98_07130"
     CDS             5234..6514
                     /locus_tag="VFF98_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HZU63437.1"
                     /translation="MSFPWANEYKPTHPFMRWVDEKLPLPRFVYNAIGAGYPVPRNLN
                     YFWNFGVLAGFCLMIQIVTGIVLAMHYAANTDVAFGSTEHIMRDVNWGWMLRYAHANG
                     ASAFFVVVYTHIFRGFYFGSYKAPREMIWLLGVVIFLLMMATAFMGYVLPWGQMSFWG
                     AQVITGLFSAIPVVGDALHTWLLGGFAPDNAALNRFFSLHFLLPFVILGVVILHIWAL
                     HIPGSSNPTGVEVKRESDTVPFHPYYTAKDGFGLAVFLFFYCALLFFFPNALGHPDNY
                     IPANPMQTPAHIVPEWYFWPFYAILRSFTADFFFIPAKLMGVLAMFSAILVWFLLPWL
                     DRSPVRSGHYRPLFKWAFRALVVDTLVLGYCGGSPAEEPYVMISQLAAAYYFLHFFVI
                     LPVVSSIETPKPLPYSITEAVLGHDEAASLAPAE"
     gene            6538..7350
                     /locus_tag="VFF98_07135"
     CDS             6538..7350
                     /locus_tag="VFF98_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HZU63438.1"
                     /translation="MIRKIAFVAGAFFSVALLYSFGKGAVSYITEPPAETVEHAFHEH
                     PKELALQSDGAFGTFDRQQLQRGFQVYNEVCKNCHALKYVAFRDLKQLGYNDDEVKAI
                     AAKYQVPAYNAATGEVKMHPGTPTDHFPPVVYAGQGSPPDLSLITKARHGGASYVYSL
                     LTGYEDQPAELLKRFPEAKTPDGLFFNPYFANLNVAMPKPLAADGQVSYKDGTKATVN
                     QMATDVSAFLVWTAEPQLEKRRQTGWAVLGFLLFASVLSYLTYRRVWAEVKH"
     gene            7514..>8494
                     /locus_tag="VFF98_07140"
     CDS             7514..>8494
                     /locus_tag="VFF98_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZU63439.1"
                     /translation="MKVLVTGAAGFIGHSLIMRLLARGDEVVGLDCVNDYYDVRLKEA
                     RLARAEAAAAPGQYTFLRQDFADYPALVAALAPYAFDRIVHLGAQAGVRYSIENPHAY
                     VSANLAGHLNLLEVARHRQVEHMVYASSSSVYGGNTKLPFAVEDRVDQPVSLYAATKK
                     ADELMSETYAHLYRLPLTGLRFFTVYGPWGRPDMMMWIFTRKIMAGEPIPVFNHGDMW
                     RDFTFIDDILAGVIASLDHPPADDGAEKAGGSTKPHRLYNIGNHRSEHLMKVVELLEA
                     ECGKRAIIDFQPIQPGDVRQSFADIAAISRDLGYAPTTGIEAGVPAFVDWY"
BASE COUNT         1354 a         2831 c         2827 g         1482 t
ORIGIN      
        1 ctgggggccg ttgacggtgg ccggatcgct gctggcgatg gcgggcatgc tgggcgcgct
       61 ctatcgccgg tcgccggccg ccgatcgccc ggcccagatc gccgccagcc cctcgcgcca
      121 ggaagggtag cgcgggcgcc agcccagcag gcgctttgcc ttgccattgg ccaccttgcg
      181 gctctcgcca taaaagccgc gcaccttggc gctgagcgcg ggatcatcgg gcgccagccg
      241 cggcggcagc ggccagccgg aaaggcggca ggcttcctgc accaccaggt ccgggtggca
      301 gggggcatcg tcggccagat tataggcgcc gggcggggcc tgccgttcca gcgcggcgat
      361 caccccgctg gcgatatcgg ccacatgcac gcgtgagaac agctgatccg gcgcggccac
      421 gcggtgggcg cggccttgtt ccagccgggc gaacatgttg cgcggggcct caccggggcg
      481 atccggccca tagatgccgg gcaggcggaa cacatgcgcg ccggccgcca gccagccggc
      541 atcggcggcc atgcgcgcat ggcgccggcc gctggcgaca ggcgctgtct cgtccaccca
      601 ggcgccggcg caatggccat agacgccggt ggaggagaga tagcccagcc agcccccccg
      661 cgcccgcagc gccgccagat ccgcgccata ggctgccagc gccggatcct ccgccgcgcc
      721 tgccccggcc actggcggca cgctggacag caccgcatcg cagcgcgcca gcagccagct
      781 gaggcgctcg gcatcggcaa aatccaccgt gcccgcgcgg ccggtcgctt ccacctgcca
      841 gccgcgcgcg cgcaagccgg cggcgatttc gcccgccgta tagccaaggc ccaggatgca
      901 gatcgtgctc attgcctttt tccataaggc caaggctggc attcgcaaac gcttgggcta
      961 gatagggcgg atgaccgatg cccgcctgca ggaaaccccg cccgccccgc ccgtgatccg
     1021 ccgcgaggaa tatcgcgtgc ccgactggct ggtgcccgaa gtggcgctgg cctttgccct
     1081 ggggctggag aagacccgcg tgttcgccac cctctcggtg cggcgcaacc cggaaggcac
     1141 gggcaaccag acgatccgcc tgcatggcga tggccttacc ccgctgctgg tggaagtgga
     1201 cgaggtggtg cgcaacgact ggcggatgga gggggcagac ctgctcatcg agctggccga
     1261 cgacacccac gagatcacca tcgaaaccga gatcaacccg gccgccaatt cgcagctgat
     1321 ggggctttat gcctcgggcg gcatgctgtg cacccagtgc gaggccgagg gcttccgccg
     1381 catcaccttc ttccccgacc ggcccgatgt gctctcgacc tatcgcgtgc ggatggaggg
     1441 cgacagggag accttcccgg tgctgctgtc gaacggcaac caggtggccg agggcgtgtt
     1501 cgaggatggc cgccactggg ccgaatggca cgatccctgg cccaagccgt cctatctctt
     1561 cgcgctggtg gccgggcggc tggtggccaa caccggccat ttcaccacca tggaaggccg
     1621 cgaggtcacg ctcaacatct ggacgcgcgc gggcaccgag ggcgaggaga cgcgcaccga
     1681 ccatgcgctg gccagcctca tcaacgccat gaagtgggac gaggaagcct ttggccgggc
     1741 ctacgacctc gatctgttca acatcgttgc cgtttccgat ttcaacatgg gggcgatgga
     1801 gaacaagggg ctcaacgtct tcaacacccg ctatgtgctg gccgacccag acaccgccac
     1861 cgatggcgat ttcgacgcgg tggaaggggt gattggccat gaatatttcc acaactggtc
     1921 gggcaaccgg atcacctgcc gcgactggtt ccagctttcc ctgaaggaag gcttcaccgt
     1981 gctgcgcgac cagatgttca gcgccgacat gggcagcccg gcggtgaagc ggatcgagga
     2041 tgtgcggatt ttgcgcagcg cccagttccc cgaggattca gggccgctgg cgcatccgat
     2101 ccgcccggat tcctatcagg aaatcagcaa tttctacaca gcgaccgtgt ataacaaggg
     2161 cgccgaggtg atccgcatga tgcgctccat ggcgggggcg gagcgcttcc gcgcgggcac
     2221 cgatctctat ttcagccgcc acgacgggga agcggccacc tgcgaggatt tcgtcagggc
     2281 gatcgaggaa ggggccgggc ttgatctcac ccgcttccgc ctgtggtatg cccaggccgg
     2341 cacgcccgag ctgaccgccc gcctcgacca cgccggcgat accgtgaccc tgcacctcac
     2401 ccagagcgtg ccggccaccc ccggccagct ggtgaagcag ccgatgccgg tgccgctgcg
     2461 catcgccctg ttcaacccgc agaccggcca gcacggcggc gagcagctga tcgtgctgga
     2521 tagcgaggag gccagcttcg ccttccaggg ctttgccagc cggccggtgc tgtcgatcaa
     2581 ccgcggcttc accgcgccgg tgaagcttgc caccgaccat cggcccgagg acctgacctt
     2641 ccttgccgcc catgatgacg atcctttcgc ccgctatgag gcgctgcaga agctgatcgc
     2701 cgatgtgttg tgcgggctgg tgaagggcca ggagcaggca gggggggcgg atcgcggcgc
     2761 gggctatgcg gcgattgccg gcgccatgcg ggcgatcatc gccgatccgg cgctggatga
     2821 cctgatgcgc ggcgagctga tgatcctgcc ggcccatgcc tatctggccg agctgctgga
     2881 gctggtcgat ccgctggccc tccatgccga gcgcgaggcg ctgaaggcgc atctgggccg
     2941 cacgctggcc gcagagctgg aagctctgca cgatcgcgcc tcggcggtgc cgttcagcct
     3001 tgcggcaggc gccaagggcg cgcgcaaggt gaagacgcag gccctcgtct atctggccgc
     3061 cggccagccc gaggcagccg ccgcccgcgc cgccgcccag tatgatgccg ccaccaacat
     3121 gaccgaccgg cagggcgcgc tgatggtgct gtgcgggctg gatacgccgc tgcgggcgca
     3181 aaaactggcg catttccacg cccgctatgc cggcaacgcg ctggtgatcg acaaatggtt
     3241 cagcctgcag gcgggctcgc tgcacaagga tgtgctggcc catgtgcagg cgctggccgg
     3301 ccaccctgat ttcaccctgc acaaccccaa ccgtgtgcgc gccctctata tggcgatggc
     3361 ggtgaacccg gcggccttcc atgcggccag cggcgagggc tatcggatga ttgccgatct
     3421 gatcctgcgg ctcgatccga tcaatgcgca gacggcggcg cgcttcgtgc cctcgctggg
     3481 gcgctggcgg cggatggcgg gcggccgctc ggccctgatg aaggccgagc tggagcggat
     3541 tgcgcaggtg ccgggcctgt cgaaggatgt gttcgagcag gtgagccgct cccttggctg
     3601 aggcgagcca gcccgaggtg ctgcgcagcc cggcgctggc gggcctgcgc catggctttc
     3661 tgggccggcg gggcggcgtt tccaccgggc tggtggcggg gctgaacgtg ggggcagggg
     3721 cgatcgggcc cgatggcgat ggcgccgcgc aagtggcgga aaaccgcgcc cgggcggtgg
     3781 cggcagtggc gccgggggcg cggctggtca cctgctatca ggtgcattcg gcgctgtgcg
     3841 tggaagcggg cgactgggct gaggcggcca ggccccatgc cgatgcgctg gtgacgcgcc
     3901 agcccggcgt ggtgctgggc gtgctgacgg ccgattgcgc gccggtgctg tttgccgatg
     3961 gagaggccgg cgttgtgggc gcggcccatg ccggctggaa gggcgccttt ggcggcgtgt
     4021 gcgaggcaac catcgcggcg atggagcaac tgggcgcggt gcgcgggcga atcgctgccg
     4081 ccatcggccc ctgcatcgcc cagccctcct atgaggtgga cgacggtttt cgcgcccgct
     4141 tttgcgcaga agcgccggcg caggcggctt ttttcgccgc cgggcgggcc gggcactggc
     4201 aattcgcgct ggaagcctat gtggccagcc ggctggcggc cgccggcatc ggccggatcg
     4261 atctgctggg gcaggatacc tatgccgatg ccgaccgctt cttctccttc cgccgcgcca
     4321 cgcaccggca agagcccgtc tatggccggc agatcagcct gatcgggctt tgaggcggcg
     4381 gccggggccg gccacgcgct tcagggcagg cccttatggc ggattgccgg ccggggccat
     4441 tggctgaagg gcccaagaaa aagtctgttg gcagtgccgg ttttgacgat tatcaaacac
     4501 aaaaagcgcc cgggcaagca gggcatacct gctatttcgt cgggcttggg ggattttcct
     4561 gaagacgcga cgatacgcgt tatgtgattc gttccagccc ttcgacccgg ggggctatct
     4621 gtgcaaggca agggtccatc atgactgaga acgccatcgc tgacactcct cccggtgcaa
     4681 acgcaggggc cggcgccgaa gacggggtgc gtcggcgcga tttcatccat atcgccgccc
     4741 tttccgccgc gggcgtgggc ggcgctgcca cgctggtgcc gctggtcagc cagatggccc
     4801 cttcggccga cgtgctggcc gaaagctcca ccgagatcga catcagccag atcaagcccg
     4861 gccaggcgat caaggcggtg ttccgcaagc agccggtgtt cgtgcgcaac ctgacgccgg
     4921 ccgagattgc cgcggccaat gcggtgccca ccgccagcct gcgcgatccc gaaacgctgg
     4981 cccagcgcac caaggccggc cacaccaact ggctgatcac catgggcgtg tgcacccatc
     5041 tgggctgcgt gccgctgggc accggccagg gcgagcccaa gggtgagttt ggcggctatt
     5101 tctgcccctg ccacggctcg caatacgaca ccgccggccg catccgcaaa ggcccggccc
     5161 ccaagaacct gcaggtgccg gaatacgctt tctcgtccgc cactgtcgtc aagatcggct
     5221 gaggtcacag atcatgagct ttccctgggc aaatgaatac aagccgaccc acccgttcat
     5281 gcgctgggtc gatgagaagc tgcctttgcc gcgcttcgtc tacaacgcca tcggcgccgg
     5341 ctatccggtg ccgcgcaacc tcaactattt ctggaacttc ggtgttctgg cgggcttctg
     5401 cctgatgatc cagatcgtca ccggcattgt gctggcgatg cattatgccg ccaacaccga
     5461 tgtcgccttt ggttccacag agcacatcat gcgcgatgtg aactggggct ggatgctgcg
     5521 ctatgcgcat gccaacggcg cctcggcctt cttcgtggtg gtctacaccc atatcttccg
     5581 cggcttctat ttcggcagct acaaggcccc gcgcgagatg atctggcttt tgggcgtggt
     5641 catcttcctg ctgatgatgg ccaccgcctt catgggctat gtgctgccct gggggcagat
     5701 gagcttctgg ggcgcgcagg tgatcaccgg gctgttctcg gccattccgg tggtgggcga
     5761 tgcgctgcac acctggctgc tgggcggttt cgcgcccgat aatgccgcgc tcaaccgctt
     5821 cttcagcctg cacttcctgc tgcccttcgt gatcctgggc gtggtgatcc tgcatatctg
     5881 ggcgctgcac atccccggct cttccaaccc caccggcgtg gaagtgaagc gcgagagcga
     5941 caccgtgccc ttccacccct attacaccgc gaaggacggg ttcggcctgg ccgtgttcct
     6001 gttcttctac tgcgcgctgc tgttcttctt ccccaatgcg ctgggccacc cggacaacta
     6061 catcccggcc aacccgatgc agacgccggc ccatatcgtg cccgaatggt atttctggcc
     6121 gttctacgcg atcctgcgct cgttcacggc cgacttcttc ttcatcccgg ccaagctgat
     6181 gggcgtgctg gccatgttca gcgccattct ggtgtggttc ctgctgccct ggctggatcg
     6241 ttcgccggtg cgttcgggcc attatcgccc gctgttcaaa tgggcgttcc gggcgctggt
     6301 ggtggatacg ctggtgctgg gctattgcgg cggttcgccg gccgaagagc cctatgtgat
     6361 gatcagccag ctggccgcgg cctattattt cctgcacttc ttcgtgatcc tgccggtggt
     6421 ctcctcgatc gagacgccca agcccctgcc ctactcgatc accgaggcag tgctgggcca
     6481 cgatgaagcc gccagcctgg ccccggccga ataagcgcgc agcgaggaga ctatcccatg
     6541 atccgcaaga tcgcattcgt cgcgggcgcc ttcttcagcg tcgccttgct ctattccttc
     6601 ggcaagggcg cggtgagcta tatcaccgag ccgccggccg aaacggtgga gcatgccttc
     6661 cacgaacacc ccaaggagct ggcgctccag tctgacggcg ccttcggcac gttcgaccgc
     6721 cagcagctgc agcgcggctt ccaggtctac aacgaggtct gcaagaactg ccacgcgctg
     6781 aaatatgtcg ccttccgcga cctgaagcag ctgggctata acgacgacga ggtgaaagcc
     6841 atcgccgcca agtatcaggt gccggcctat aatgccgcca ccggcgaggt gaagatgcac
     6901 ccgggcacgc ccaccgatca tttcccgccg gtggtctatg ccgggcaggg cagcccgccc
     6961 gatctcagcc tgattaccaa ggcgcgccat ggcggggctt cctatgtcta ttcgctgctg
     7021 accggttacg aggaccagcc cgccgagctg ctgaagcgct tccccgaagc caagacgccc
     7081 gacggcttgt tcttcaaccc ctatttcgcc aacctcaacg tggcgatgcc caagccgctg
     7141 gcggccgatg ggcaggtgtc gtacaaggat ggcaccaagg cgacggtgaa ccagatggcc
     7201 accgacgttt ccgccttcct ggtgtggacg gccgagcccc agctggaaaa gcgccggcag
     7261 acgggctggg cggtgctggg cttcctgctg tttgcctcgg tgctttccta tctcacctat
     7321 cgccgggtgt gggctgaagt gaagcactag gccacggcca aaccggattg ccgggcacag
     7381 ccaccccggc atgcaccgaa cgcccctcgc attcagccgt gcggggggcg tttgtgtggg
     7441 cgggcacaaa tcctttatct ttcgggccta tcgggggatg aaaggtgtat caggcagaag
     7501 gcgggttgtg ggcatgaagg ttctggttac gggcgctgcg ggtttcattg gccacagcct
     7561 gatcatgcgg ctgctggcgc gcggcgacga ggtggtgggc ctcgattgcg tgaacgacta
     7621 ttacgacgtg cggctgaaag aggcccgcct ggcccgcgcc gaggccgccg ccgcgcccgg
     7681 ccaatacacg ttcctgcggc aggatttcgc cgattatccg gcgctggtgg cagcgctggc
     7741 gccttatgcc ttcgatcgca tcgtgcatct gggggcgcag gcgggggtgc gctattcgat
     7801 cgagaacccg catgcctatg tttcggccaa tctcgccggc catctgaacc tgctggaagt
     7861 ggcccgccac cggcaggtgg aacatatggt ctatgcctct tcctcctcgg tctatggcgg
     7921 caacaccaag ctgccctttg ccgtggaaga ccgggtggac cagcccgtca gcctctatgc
     7981 cgccaccaag aaggccgatg agctgatgag cgagacctat gcccatctct atcgcctgcc
     8041 gctcaccggg ctgcgcttct tcaccgtcta tggcccctgg ggccggcccg acatgatgat
     8101 gtggatcttt acgaggaaga tcatggccgg cgagccgatc ccggtgttca accatggcga
     8161 tatgtggcgc gatttcacct tcatcgacga tatcctcgcc ggggtgatcg ccagtctcga
     8221 tcatccgccg gccgacgatg gcgccgaaaa ggccggtggc agcaccaagc cgcaccggct
     8281 ctacaacatc ggcaaccacc gctccgaaca tctgatgaag gtggtggaac tgctggaggc
     8341 tgaatgcggc aagcgggcga tcatcgactt ccagccgatc cagccgggcg atgtgcgcca
     8401 gagctttgcc gatatcgcgg cgatctcgcg cgatctgggc tatgccccca ccacgggcat
     8461 cgaggcgggg gtgccggcct tcgtcgattg gtac
//
LOCUS       DASRYE010000128         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_78056,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000128 DASRYE010000000
VERSION     DASRYE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78056"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFF98_07145"
     CDS             <1..730
                     /locus_tag="VFF98_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZU63440.1"
                     /translation="LAALQRWSERPRGALPAAAGATDPGLRLASERETLDWLATQGVP
                     VVPCRLATTAGAARAAFAAIGGPVVLKIASPDIAHKSDIGGVRLNLASAEATAAAFDE
                     VMAAARAHHPAARLEGCLVAPMRAAGLELFVGTARTAWGMMLAVGLGGVWIEVLADTA
                     LRPLPVSPAEVTEMLGTLRGSRLLAGHRGSPPADIPALARAIAAIGDAALALGPALVS
                     LEVNPLRVSGGEVEALDALAVWDD"
     gene            763..1665
                     /locus_tag="VFF98_07150"
     CDS             763..1665
                     /locus_tag="VFF98_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63441.1"
                     /translation="MDGPDLKALRMFEAVARHLSFTRAAEELCVAQPWLSVQLRRLEE
                     QLGFALFARGRKRAVVLTDEGEALLPHARALAASHQRLCAEASRIRKGQRRRLVLGAP
                     EFSADIAARQQLLAALRARGDIEVEIVNGPSTALIDHLRQGRVDITLALGPLPAEADL
                     EACVLAQTGLWLALPDGHPLAAMAAVPMALLAGQRIANFRRRLNPQVYDHMARLFAEA
                     GVEVEHLPEASARAVMLFVAQEQTPVIVTQWMAPLLAGSQVVLRPVADAPLRLQLLLL
                     RRRGDARAGVAALWACAEALEEGV"
     gene            complement(1679..3199)
                     /locus_tag="VFF98_07155"
     CDS             complement(1679..3199)
                     /locus_tag="VFF98_07155"
                     /inference="COORDINATES: protein motif:HMM:NF036848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZU63442.1"
                     /translation="MTAPSPAHVPVIVGVGEISDRLADGHEGLEPRDLIAAAARRADA
                     DAGGGWLSSIDTVAVVNILSWPYADICASLAAALGIAPARAEELPAGGNVALKAIHDI
                     ANRIAGGAEETALVCGAEAFRSVALARKAGRTPGWTAPDSGHWIKTMVDVVGPDMLRY
                     GFDGAPSVYALYENAMRAARGLSHAEAQAQTGAMWAAMSQVAAGVEEAWIRTPRAAAE
                     IITPGPDNRPIAFPYTKLMVANDAVNQGSAVIVTSLARARAAGVEEGRIAYVWGGAAA
                     RDRRDFRTRDRYDHSPSIEAVIAATLAQSGLAASAFDAVEFYSCFPCVPRLAQGASGI
                     DPAIVPTVAGGLTYFGGPGNDYMTHAVINMVRQLRAGKRLGLLLGNGDLVTKHHAMIL
                     GASPPPAGTLPVAYDVQADAEARRGPVPEVIERHEGPALLETYTVKYRRDGTPDYGLA
                     VCRTPQGARFVARVPGSDERTIAALVGDGGGWVEPIGQEGVAIAHGAEDFCEWRLG"
     gene            complement(3219..4748)
                     /locus_tag="VFF98_07160"
     CDS             complement(3219..4748)
                     /locus_tag="VFF98_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU63443.1"
                     /translation="MREALDAACWPDRLALRFGERLALADGARQVSYADLAARSAALA
                     GALAARGLAAGDRVAALMLNGAPLIELYLAAARLGAIVLPLNWRLAPAELGWILGNAA
                     PRLWFVSEGLADLAEAIAPGLDTVMVTDGAAGADGYDALLAEGHGAPPSPADADERPW
                     IMLYTSGTTGRPKGCLLSQRGQMISLLAMMGQWGLQPGEGLLLSLPLFHVGGTGVLFA
                     ALASGARILVAPRQFGAEQAYRLIADEACVAAAIVPQYYEGLIACHRREQAPLALRIA
                     SAGGGMHDPAFIEEIRTALGTELLLGYGQTEAGNYIAYLRGEEQLRAPKACGRLLPHL
                     DMRVAGEGDAPLPPGETGELLVRGPGIFERYWQADEASEATLRGGWLHTGDLMRLDGD
                     GLLTLVGRAKELIKTGGENVYPREVEAVLLEHPDIVDCAVFGVAHDYWGESVKAMVAL
                     RPGAALRPADVASWCRQHIAAYKRPRFVEFVAAMPRTDTGKLVTRDLRARPVTADQAT
                     D"
     gene            complement(4754..>5007)
                     /locus_tag="VFF98_07165"
     CDS             complement(4754..>5007)
                     /locus_tag="VFF98_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63444.1"
                     /translation="FHQSMVDRTVMDWAGPLAFLTRRKIAMLDQICPGDRIEGHARVE
                     RVYRGEKGEGLVDFTVEITRPGTVCCRGWATLRLPERAG"
BASE COUNT          708 a         1857 c         1753 g          689 t
ORIGIN      
        1 gctggcagcg ctgcagcgct ggtcggaacg gccgcgcggg gccctgcccg cggccgccgg
       61 tgccaccgat ccgggcctgc gccttgcctc cgaacgcgag acgctggact ggctggcaac
      121 gcagggcgtg ccggtggtgc cctgccgcct ggccaccacc gccggcgcgg cgcgcgccgc
      181 ctttgccgcc atcggcgggc cggtggtgct gaagatcgcc tcgcccgaca tcgcccacaa
      241 aagcgacatc ggcggcgtgc ggctgaacct ggccagcgcc gaggccaccg ctgccgcctt
      301 cgacgaggtg atggccgccg cccgcgctca ccaccccgca gcgcggctgg aaggctgcct
      361 ggtggccccc atgcgggcgg cggggctgga actgttcgtc ggcaccgcgc gcactgcctg
      421 gggcatgatg ctggcggtgg ggctgggcgg cgtgtggata gaagtgctgg ccgataccgc
      481 cctgcgccca ctgcccgtca gccccgccga ggtaacggag atgctgggca ccttgcgcgg
      541 cagccgccta ctggccggcc atcgcggcag cccgccggcg gatatccctg cccttgcccg
      601 cgccattgcc gccattggcg atgcggcgct ggcgctgggg ccggcgctgg tctcgctgga
      661 agtgaacccg ctccgggttt cgggcggcga ggtggaggcg ctcgacgcgc tggcggtgtg
      721 ggatgattga tcgacgcggc ccgcccgcct agaaccgccg gcatggacgg gcccgatctc
      781 aaagcgctgc gcatgttcga ggcggtggcc cgccacctct ccttcacccg cgcggcagag
      841 gaattatgcg tggcccagcc ctggctgtcg gtgcagctgc gccggctgga agagcagctg
      901 ggctttgccc tcttcgcgcg cgggcgcaag cgggcggtgg tgttgaccga cgagggcgag
      961 gcgctgctgc cccatgcccg cgcgctggcc gccagccacc agcgcctgtg cgccgaggcc
     1021 agccgcatcc gcaagggcca gcggcggcgg ctggtgctgg gcgcgccgga attcagcgcc
     1081 gatatcgccg cccgccagca gctgctggca gccctgcgcg cgcgcggcga tatcgaggtg
     1141 gagatcgtca acggcccttc caccgcgctg atcgaccatt tgcgccaggg ccgggtggat
     1201 atcaccctgg cgctgggccc gctgccggcc gaggccgacc ttgaggcctg cgtgctggcg
     1261 cagaccggcc tgtggctggc cctgcccgat gggcatccgc tggccgccat ggccgcggtg
     1321 ccgatggcgc tgctggccgg ccagcgcatc gccaacttcc gccgccggct gaacccgcag
     1381 gtctacgacc acatggcccg cctgttcgcc gaggcggggg tggaggtgga acacctgccg
     1441 gaagcctcgg cgcgggcggt gatgctgttt gtggcgcagg agcagacgcc ggtgatcgtg
     1501 acccagtgga tggccccgct gctggccgga tcgcaggtgg tgctgcgccc ggtggccgat
     1561 gccccgctgc gcctgcaact gctgctgctg cgccggcggg gcgatgcgcg cgccggggtg
     1621 gcggcgctgt gggcctgtgc cgaggcgctg gaggagggcg tttagcgcgc ccctcccctc
     1681 agcccagccg ccactcgcag aaatcctccg cgccatgggc gatggccacg ccctcttggc
     1741 cgatcggctc cacccagccg ccgccatcgc ccaccaatgc ggcgatcgtg cgttcatcgc
     1801 tgcccggcac gcgcgccaca aagcgcgccc cctgcggcgt gcggcacacc gcaaggccat
     1861 aatcgggggt gccatcgcgg cggtatttca ccgtgtaggt ctccagcagc gccgggcctt
     1921 cgtgccgctc gatcacctcg ggcacggggc cgcggcgggc ctcggcatcg gcctgcacgt
     1981 cataggccac cggcagcgtg ccggccggcg gcgggctggc gcccaggatc atcgcatggt
     2041 gcttggtgac cagatcgcca ttgcccaaaa gcagccccag ccgcttgccg gcccgcagct
     2101 ggcgcaccat gttgatcacc gcatgggtca tgtaatcatt gcccggcccg ccgaaatagg
     2161 tgagcccgcc agccaccgtt ggcacgatgg ccggatcgat gccgctggcg ccctgggcca
     2221 ggcgcggcac gcaagggaag caagaataga actccaccgc atcgaaggcg ctggccgcaa
     2281 ggccgctctg ggcaagggtg gcggcgatca ccgcctcgat cgagggggaa tggtcgtaac
     2341 ggtcgcgcgt gcggaaatcg cgccggtcgc gcgcggcagc gccgccccag acataggcga
     2401 tgcgcccctc ctccacgccc gccgcccgcg cccgcgccag gctggtaacg atgacggcag
     2461 agccctggtt caccgcatca ttggccacca tcagcttggt gtaggggaag gcgatggggc
     2521 ggttgtcggg cccgggggtg atgatctcgg cggccgcgcg gggagtgcgg atccaggctt
     2581 cctccacccc tgccgccact tgcgacatcg ccgcccacat ggcccccgtc tgcgcctgcg
     2641 cctcggcatg gctgaggccg cgcgcggcgc gcatggcgtt ctcatacagg gcatagacgc
     2701 tgggcgcgcc atcgaagccg tagcgcagca tgtccggccc caccacatcc accatggtct
     2761 tgatccagtg gccgctgtcg ggcgctgtcc agccgggcgt gcggccggcc ttccgggcca
     2821 gcgccaccga gcggaaggcc tctgccccgc acaccagcgc cgtctcctcg gccccgccgg
     2881 cgatgcggtt ggcgatatcg tgaatggcct tgagcgccac attgccgccg gcgggcagtt
     2941 cctcggcgcg ggcaggggca atgcccagcg ccgctgccag gctggcgcag atatcggcat
     3001 agggccagga caggatgttg acgaccgcca ccgtgtcgat gctggaaagc cagccgccgc
     3061 cggcatcggc atcggccctg cgggcggccg cggcgatcag gtcgcgcggc tccagccctt
     3121 catggccatc ggccagccgg tcggaaattt cgcccacgcc aacgatcacg ggcacatggg
     3181 cgggagaagg ggctgtcatc cgggatgggt tcctttggtc agtcggtggc ctgatcggcc
     3241 gtgacggggc gcgcacgcag atcacgcgtg acgagcttgc cggtatcggt gcgcggcatc
     3301 gccgccacga attcaacgaa acgcgggcgc ttgtaggcag cgatatgctg gcggcaccag
     3361 ctggccacat cggccggccg cagggcagcg cccgggcgca gcgccaccat cgccttcacg
     3421 ctttcgcccc aatagtcatg cgccacgccg aacaccgcgc aatcgacgat atccggatgc
     3481 tccagcagca ccgcctccac ctcgcgcggg tagacattct cgccgccggt cttgatcagc
     3541 tccttggcgc ggcccacaag ggtgagcagg ccatcgccat ccagccgcat cagatcgccg
     3601 gtgtgcagcc agccgccgcg caaggtcgcc tcgctggcct cgtcggcctg ccagtagcgc
     3661 tcgaagatgc cggggccgcg caccagcagc tcgcccgtct cgcccggtgg cagcggggca
     3721 tcgccctcgc cggccacgcg catgtcgaga tggggcagca ggcgcccgca ggccttgggc
     3781 gcgcgcaact gctcctcgcc gcgcagatag gcgatgtaat tgccggcctc tgtctggcca
     3841 tagcccagca gcagctcggt gccgagcgcc gtgcggattt cctcgatgaa ggcgggatca
     3901 tgcatgccgc caccggccga ggcgatgcgc agcgccaggg gcgcctgctc gcgccggtgg
     3961 caggcgatca gcccctcgta atattgcggc acgatggcag cggccacgca ggcctcgtcg
     4021 gcgatcaggc gataggcctg ttccgccccg aactggcgcg gcgcgaccag aatgcgcgcg
     4081 cccgaggcca gcgccgcgaa cagaacgccc gtgcccccca catggaacag cggcagcgac
     4141 aagagcagcc cctcgcccgg ctgcaacccc cattgcccca tcatcgccag cagcgagatc
     4201 atctgcccgc gctggctgag caggcagccc ttgggccgcc cggtggtgcc gctggtgtag
     4261 agcatgatcc acggccgctc gtcggcatcg gcgggcgacg ggggggcccc gtgcccctcg
     4321 gccagcagcg cgtcatagcc atcggcgccg gccgcgccat ctgtcaccat caccgtatcc
     4381 aggcccggcg caatcgcctc ggccagatcc gccagccctt cagagacgaa ccacagccgc
     4441 ggcgccgcat tgcccaaaat ccagcccagc tcggccgggg ccagccgcca gttcagcggc
     4501 agcacgatcg cccccagccg ggcggcggcc agatacagct cgatcagcgg cgcgccgttc
     4561 agcatcagcg cggccaccct gtcaccggcg gccagcccgc gcgcggcgag cgcccccgcc
     4621 agcgccgccg agcgcgccgc cagatcggca tagctgacct gccgcgcgcc atcggccagc
     4681 gccagccgct cgccaaagcg cagcgccagc cggtctggcc agcaggcggc atccagggcc
     4741 tcgcgcatgg ccatcagcct gccctttccg gcaggcgcaa ggtggcccag ccgcggcagc
     4801 acacggtgcc cggcctggtg atctccaccg tgaaatcgac cagcccctcg cccttctcgc
     4861 cgcgatagac ccgctcgacc ctggcatggc cctcgatccg gtcgccgggg cagatctggt
     4921 cgagcatggc gatcttgcgg cgggtgagga aggcgagcgg cccggcccag tccatcaccg
     4981 tgcgatcgac catgctctgg tggaaca
//
LOCUS       DASRYE010000129        11720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_57959,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000129 DASRYE010000000
VERSION     DASRYE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57959"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFF98_07170"
     CDS             <1..643
                     /locus_tag="VFF98_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU63445.1"
                     /translation="FPGPEGRLEGRFQPGTRPRAPVAMILHPHPQAGGTMNDRITQAL
                     YKTFVARGFATLRFNFRGVGRSQGSFDNGIGELSDAAAALDWVQSIHPEASTTWIAGY
                     SFGALIGMQLLMRRPEIRGFISVAPPANMYDFSFLAPCPASGIIVQGTSDTVVAPGAV
                     QKLVDKLRTQKHITIHHDEVPRANHFFENELDDLMRSVDNYLDMRLSPDCPIK"
     gene            complement(701..1207)
                     /locus_tag="VFF98_07175"
     CDS             complement(701..1207)
                     /locus_tag="VFF98_07175"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HZU63446.1"
                     /translation="MVMLATLRRSGGIEAMARKLGAPPAMVAMATAVLMPQLLAGMAR
                     IVRKAAGSTERADLAAGVAHLVRRLERAGGGSLAVAVMMPEPLSPEQGRRLLADICGT
                     PEKLAGMITHCADEAALERGFVSELLPLLTMLVGGYLAARAEVLAVSDGDLATEFLLL
                     LEMENPAG"
     gene            complement(1436..3049)
                     /locus_tag="VFF98_07180"
     CDS             complement(1436..3049)
                     /locus_tag="VFF98_07180"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HZU63447.1"
                     /translation="MTASLSTPLDRQGIATDAQIFANLGTSPLVEHAVRNGEGLLAKD
                     GPFVVATGKHTGRSAKDKFIVKDAETENTVWWGKTNVAMSPEHFAALKADFFAELAKR
                     DKLYVADLFGGSQPEFRVNVRVINELAWHNLFIRTLLVRPSEAELASFAPEYTIIDLP
                     SFRADPARHGCRTETVIAVNFTEKLILIGGTAYAGEMKKSVFGILNYLLPTKGVMPMH
                     CSANIGPDGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMI
                     RLSAEAEPEIFATTKRFGTVLENVVIDPETRTLDLDDATLAENSRGSYPIDFIPNASA
                     DNLGPVPQNIIFLTADAYGVLPPIARLTPEQAMYHFLSGYTARVAGTEIGVTEPEATF
                     STCFGAPFMPRHPSIYGNLLKERIAKGGVKCWLVNTGWSGGKATMEGIKRMPIKATRA
                     LLNAALDGSLNEATFKKDPNFGFEVPVAVEGVETRLLDPRGAWADAAEYDKTAADLVN
                     KFVENFAQFADHVDEGVRAAAPAVGTVEA"
     gene            3263..4084
                     /locus_tag="VFF98_07185"
     CDS             3263..4084
                     /locus_tag="VFF98_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU63448.1"
                     /translation="MTGAPSAPSGPPATPPAPAGEAAPGTPARHADALAPASRHTIAL
                     VDDDRNILTSVSIGLQAEGFATRLYADGASALKALLENPPDLAVFDIKMPRMDGLELL
                     RRLRAKSALPVIFLTSKDGEPDEALGLAMGADDYIAKPFSQGLLVARIRAILRRVELA
                     RQDALGPPAAALPEPVRRGRLSMDPARHEVLWDGRAVSLTVTEFLILEALAQRPGVIK
                     TRSQLMDAAYADDIYVDDRTVDSHIKRLRRKFRAVDTGFAAIETLYGAGYAFAES"
     gene            4065..5648
                     /locus_tag="VFF98_07190"
     CDS             4065..5648
                     /locus_tag="VFF98_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU63449.1"
                     /translation="MPLPRAEHARRSRWRGVSLTTRILAVNVIVLGLMGFSLFYLDSY
                     RKQLVRERFAQASSDAAIIADALALTSPEGRAAVLSRIAARQHMRLQIFGPHGEELAD
                     SRPVAGPGFVLINPHTQPWYMKAARTLDRAMDAAVNAPPIPDYADIDDTSGWPEVEQA
                     RSSNRVIVWERYAPDRSPVITTGSALGHGGEVLFTRRNGRDITQNVRDARQTLAIVVG
                     VALLVTVFLSLFLARTIVQPLRMLVRAAVRVRLGRDRAVVVPRLPQRGDEIGLLARAL
                     ADMSEALRQRIDAVESFAADVAHEIKNPLASLRSALDTLDKVEDPELRRQLADIAAHD
                     VQRIDRLVTEIADASRIDAELSRTTFELLDMAALARALVGARAMRGVNGGRKLRVFAP
                     DGQEAPIVAGDPARLERVLENLLDNAVSFSPEGGTITITIRTEGAQVELEVTDEGPGI
                     AAEARERVFERFHSLRPAGEDFGHHSGLGLAIARTIIEAHDGTLVAEEPLPGAPGRPP
                     GARLVIRLPAYSPRGSLLG"
     gene            5645..6115
                     /locus_tag="VFF98_07195"
     CDS             5645..6115
                     /locus_tag="VFF98_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HZU63450.1"
                     /translation="MNAAGNSPAPQGQAHQATAVAINGRAVLIEGPPGAGKSTLALAL
                     IDRGAVLVGDDGVWLRAERGRLWAAPHPRTQGLIEVRNLGILAAADLGWPVCPQAPVA
                     LVLVLDVAAPRFIEAPEARQIAGIGLPQLRLWPGGEGLALKAELALARHGLAAI"
     gene            6206..7177
                     /gene="rapZ"
                     /locus_tag="VFF98_07200"
     CDS             6206..7177
                     /gene="rapZ"
                     /locus_tag="VFF98_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZU63451.1"
                     /translation="MLPDSAIAAPAPAKPGAHKQKLLLVTGVLGAGKSTALRALEDLG
                     WEAIDNFPIRLLERLIDGPDGNGAPAAGGSEWPAPLAVGFDSRTRGFNPHTVVELVKK
                     LSQRDDLEISTLFLDCAVDELQRRYNETRRRHPLAADMPVSAGIAAERELMEPLRRWA
                     EVVVNTTAFSTHQLSQAIREQFSGEAGPNITVTITSFGFARGMPPVADFVFDMRFLNN
                     PHWHEDLRPHTGREENVAAFIRQDPAFEEAFTRIRDLLLLVLPRFQAQGKAYAHIAFG
                     CTGGKHRSVFMAEQIGAALRDAGFAPNILHRNLGARATNLIEGSPAR"
     gene            7288..7692
                     /locus_tag="VFF98_07205"
     CDS             7288..7692
                     /locus_tag="VFF98_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZU63452.1"
                     /translation="MIGLILVTHGKLAEEFVHAMEHVVGRQEAVATVCIGPNDDVERR
                     RREIADAIRVVDSGHGAIILTDLFGGTPSNLAISLMEAGRVEVIAGINLPMLIRLAGS
                     RKTMDVRAAVSAARDAGRNYITVASEFLGQDL"
     gene            7707..7985
                     /locus_tag="VFF98_07210"
     CDS             7707..7985
                     /locus_tag="VFF98_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZU63453.1"
                     /translation="MAEARQSVEIVNAKGLHARASAKFVNMCASLPADVSVTVVKDGA
                     EAAGGSILGLMMLGAAKGDTIEISVAGAEADTVLARLAGLVLDGFGED"
     gene            7996..8811
                     /locus_tag="VFF98_07215"
     CDS             7996..8811
                     /locus_tag="VFF98_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZU63454.1"
                     /translation="MTQTVRRTITGFSNPTVKFLKSLRDKKHRRREGKFLAEGLRLLT
                     DARESGRLPEMLVMARGREAHPLLAELEAAVAAAGGEVVEMEEEILAKVTGKDNPQAV
                     AGVFAEFDTSLAGIDRQAAPIWLVAQAMRDPGNLGTMLRTGDAVGAGGLILLDDCVDP
                     FSVEAVRASMGAIFTQRLAMARWDDFLPWLRAGAGQLVAASLRDAVPYRGAPYQGPVF
                     IMVGNESRGLPEAYEPECDLRVTMPMKGRADSLNAAVAAAVLAYEVLAGLEAG"
     gene            complement(8838..10262)
                     /gene="rmuC"
                     /locus_tag="VFF98_07220"
     CDS             complement(8838..10262)
                     /gene="rmuC"
                     /locus_tag="VFF98_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZU63455.1"
                     /translation="MDSVALVGVVLGLGGGAGLGWFVGARGAAGWRARHDAIAADLRT
                     LDERFRRAIAELAGASERASRADGLEGELRRAATEREGLRAELATLRAHAAHFEEQKR
                     LLVEAQEGLKAQFEASGARVLAKAQEAFLARAEARFTESEKTSEERLRAVLAPVSHRL
                     RAYEEQVQGLEARRVDAFGQLLGQIEQLRLGQEQVKAEAARLGNSLRNAPKARGRWGE
                     QQLRNVLEQCGLAEHTDFVTEKSIDTEEGRLRPDAIVNIPGKKKLVIDAKVSLNAYQE
                     AFEAEDEAAREAALRLHALSMRTHIQTLASKSYQSQFEEAPDYVLMFVPGEHFIAAAL
                     EFDRDIWNFAFERKVLLASPTNLVAIARTVAQVWRQDGLAREAREIGRMGGELYDRLA
                     VAAEHLKRVGGGLTTAVDNYNKFVGSFERNVLSAGRRLRDKHIEIGKREIEDVALVES
                     APRYADAGAGDPGEVPALPEAQEV"
     gene            complement(10316..10882)
                     /locus_tag="VFF98_07225"
     CDS             complement(10316..10882)
                     /locus_tag="VFF98_07225"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA];
                     GO_process: GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA];
                     GO_process: GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZU63456.1"
                     /translation="MAIRPIVEAPDPRLKQVSAPVTAFDEGLKTLVADMFETMYDAPG
                     IGLAAIQVGEAVRVVVIDLQEPDEDAAPEACEDDGCGHDHRPVKKNPQVFINPEILDP
                     SEDYAVYNEGCLSVPDIFAEVERPATCRLRWQDLDGATHEAAMEGLMATCIQHEMDHL
                     EGILFIDHLSRLKRNMALKKLEKARKAA"
     gene            complement(10952..11548)
                     /gene="recR"
                     /locus_tag="VFF98_07230"
     CDS             complement(10952..11548)
                     /gene="recR"
                     /locus_tag="VFF98_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZU63457.1"
                     /translation="MASQEIEALAASLARLPGLGPRSARRAVLWLIKRRETALEQLLE
                     ALATLRERLAECRICGNVDTVSPCAICTDPRRDQRSLCVVEDVADLWALDRSRLFTGR
                     YHVLGGRLSALDGVRPEDLSIGPLLERVRAGGIDEVVLAMNATLEGQTTAHYIAERLE
                     GLPVRVTQLAHGLPVGGELDYLDEGTLAQALRARRPVG"
     gene            11602..>11720
                     /locus_tag="VFF98_07235"
     CDS             11602..>11720
                     /locus_tag="VFF98_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZU63458.1"
                     /translation="MRIIYMGTPEFAVPALEALVAAGHDVVAAYSQPPRPGGRR"
BASE COUNT         1849 a         4144 c         3838 g         1889 t
ORIGIN      
        1 ctttcccggc ccggaaggcc gccttgaagg ccgctttcag ccgggcaccc gcccgcgcgc
       61 gccggtggcg atgatccttc atccgcaccc gcaggccggc ggcaccatga acgaccgcat
      121 cacccaggcc ctctacaaaa ccttcgtggc ccgcggcttt gccacgctgc gcttcaattt
      181 ccgcggtgtt ggccgctcgc agggcagctt cgacaatggc atcggcgaat tgtcggatgc
      241 cgccgccgcg ctcgactggg tgcagtccat ccaccccgag gccagcacca cctggatcgc
      301 cggctattcc ttcggcgccc tcatcggcat gcaattgctg atgcgccggc cggaaatccg
      361 cggcttcatc tcggtggcgc cgccggccaa catgtatgat ttcagcttcc tggcgccttg
      421 cccggccagc ggcatcatcg tccagggcac gtccgacacg gtggtggcgc cgggcgctgt
      481 gcagaagctg gtggacaagc tgcgcaccca gaagcacatc accatccacc acgacgaagt
      541 gccccgcgcc aaccatttct tcgagaacga actcgacgat ctgatgcgct cggtcgacaa
      601 ttacctcgac atgcgcctgt cgcccgattg cccgatcaag taagcgattg cgggtaaggc
      661 gggggccgcg cgccctcgcc cggcccgccc cgctggcgca tcagccggcg gggttttcca
      721 tttccagcag cagcaggaat tcggtggcca gatcgccatc ggaaaccgcc agcacctcgg
      781 cccttgccgc cagatagccg cccaccagca tcgtcagcag cggcagcagc tcgctgacaa
      841 agccgcgttc cagcgcggcc tcgtcggcgc aatgggtgat catgccggcc agcttttcgg
      901 gcgtgccgca aatatcggcc agcaaccggc gcccctgctc tggcgagagc ggctcgggca
      961 tcatcaccgc cacggccagc gaaccgcccc cggcccgttc cagccggcgc accagatgcg
     1021 ccacgcccgc ggccagatcc gcccgctccg tgctgccggc agccttgcgc acgatgcgcg
     1081 ccatgccggc cagcaattgt ggcatcaaca cagcggttgc catggcaacc atggctggcg
     1141 gtgcgcccag tttacgcgcc atcgcctcta tgccgccgct acggcgcagc gtggccagca
     1201 ttaccattat ccactcccca ccccttcgga gagatgcaac ccggtaacat atctaaccaa
     1261 ttgcgggtcg tggggatagg tattttcaac gggttggtga atttatgcga aagaatcgcg
     1321 ataacaggcg gattcttgcg caatccaggc gctacacagc agccccacaa ggcaaaggcc
     1381 ccgccggcaa acgccagcgg ggccctccct gaacctgttg caatgcggct gtggatcagg
     1441 cctcgaccgt gccgacggca ggggcagcgg cgcgcacgcc ctcgtccaca tggtcggcga
     1501 actgggcgaa gttctcgacg aacttgttca ccagatcggc cgcggtcttg tcatattcgg
     1561 ccgcatcggc ccaggcgccg cgcgggtcga gcaggcgggt ttccacgcct tccaccgcca
     1621 ccggcacttc aaagccgaag ttggggtcct tcttgaaggt cgcctcgttc aggctgccat
     1681 ccagcgcggc gttgagcagg gcgcgggtgg ccttgatcgg catgcgcttg atgccttcca
     1741 tggtggcctt gccgcccgac cagccagtgt tcaccagcca gcacttcacg ccgcccttgg
     1801 cgatgcgctc tttcagtagg ttgccataga tgctggggtg gcgcggcatg aagggggcgc
     1861 caaagcaggt ggagaaggtg gcttccggct cggtcacgcc gatctcggtg ccggccacgc
     1921 gcgcggtgta gcccgagagg aagtgataca tcgcctgctc gggcgtgagg cgggcgatcg
     1981 gcggcagcac gccataggca tcggcggtga ggaagatgat gttctgcggc accgggccca
     2041 gattgtcggc gctggcgttg gggatgaagt cgatcggata ggaaccgcgg ctgttctcgg
     2101 ccagcgtggc atcgtcgagg tcgagcgtgc gggtttccgg gtcgatcacc acattttcca
     2161 gcaccgtgcc gaagcgcttg gtggtggcga agatttccgg ctcggcctcg gccgacaggc
     2221 ggatcatctt ggcatagcag ccgccctcga agttgaacac ggcggtatcc gaccagccat
     2281 gctcgtcatc gccgatgagg gtgcggctgg catcggccga cagcgttgtc ttgccggtgc
     2341 ccgacaggcc gaagaacacg gcggtgtcgc catcggggcc gatgttggcc gagcagtgca
     2401 tcggcatcac gcccttggtg ggcagcagat agttgaggat gccgaacacc gacttcttca
     2461 tctcgccggc ataggccgtg ccgccgatga ggatcagctt ctcggtgaag ttcaccgcga
     2521 tcacggtttc ggtgcggcag ccgtggcggg ccggatcggc gcggaagctg ggcaggtcga
     2581 tgatcgtgta ttcgggcgca aagctggcca gctcagcctc gctggggcgc accagcaggg
     2641 tgcggatgaa caggttgtgc caggccagct cgttgatcac gcgcacgttc acgcggaatt
     2701 cgggctgcga gccgccgaac agatcggcga catagagctt gtcgcgcttg gccagctcgg
     2761 cgaagaaatc cgccttcagc gcggcaaaat gctccgggct catcgccacg ttggtcttgc
     2821 cccaccacac ggtgttctcg gtttcagcgt ccttgacgat gaacttgtcc ttggcggaac
     2881 ggccggtgtg cttgccggtg gccaccacaa aggggccatc cttggccagc aggccttccc
     2941 cgttcctcac cgcatgttcc accagcggcg aggtgccgag gttggcgaag atctgggcat
     3001 cggtggcaat gccctggcgg tcgagcgggg tgctgagcga ggcagtcaag gtcgtctccc
     3061 gtgagaagcg cggacgcggc caggctggca acgcctcgcg catacgaatg cgatacgtcg
     3121 aatcggcata atccagccgc gcgcgctcgt caaatggccc aaacacgcgc cccctcgggc
     3181 tgacgcaagc ctgccacacc atggcccgca acggcgccgc cagttgcctg cgcccccgcg
     3241 acgcgctagg cccatgccgg atatgacagg agccccttcc gccccgtccg gcccgcctgc
     3301 cacgcccccg gcccctgccg gcgaggcggc ccccggcacg ccagcgcgcc acgcagatgc
     3361 gctggcgccc gcttcgcgcc acaccatcgc cctggtcgat gatgatcgca acatcctcac
     3421 cagcgtctcc atcggcctgc aggccgaggg ctttgccacc cgcctctatg ccgatggcgc
     3481 cagcgcgctg aaggcgctgc tggaaaatcc gcccgatctg gccgtgttcg acatcaagat
     3541 gccgcgcatg gacgggctgg agctgctgcg ccgcctgcgc gccaaatcgg cgctgccggt
     3601 gatcttcctc accagcaagg atggcgagcc cgacgaggcg ctgggcctgg ccatgggggc
     3661 cgacgactat atcgccaagc ccttctcgca agggctgctg gtggcgcgca tccgggcgat
     3721 cctgcgccgg gtggagctgg cccggcagga tgcgctgggc ccgccggccg cagccctgcc
     3781 ggaacctgtg cgccgcggcc ggctcagcat ggatccggcc cgccacgagg tgctgtggga
     3841 tggccgggcg gtgtcgctca cggtcaccga atttctcatt ctggaagcgc tggcccagcg
     3901 cccgggcgtg atcaagaccc gcagccagct gatggacgcg gcctatgccg acgacatcta
     3961 tgtggatgat cgcacggtcg attcccacat caagcgcctg cgccgcaagt tccgcgcggt
     4021 cgacaccggc tttgccgcga tcgagacgct ctatggcgcc ggctatgcct ttgccgagag
     4081 ctgagcacgc gcgccgctcg cgctggcggg gggtttcgct caccacccgc atcctggcgg
     4141 tgaacgtgat cgtgctgggg ctgatgggct tcagcctgtt ctacctcgac agctatcgca
     4201 agcagctggt gcgcgaacgc ttcgcccagg ccagcagcga tgccgcgatc atcgccgatg
     4261 ccctggcgct gaccagcccc gaggggcgcg cggcggtgct ctcgcgcatt gccgcgcgcc
     4321 agcacatgcg cctgcagatc ttcggccccc acggcgagga actggccgac agccggccgg
     4381 ttgccggccc cggcttcgtg ctgatcaacc cgcataccca gccctggtac atgaaggccg
     4441 cccgcacgct cgaccgggcg atggatgccg cggtgaacgc gccgcccatc cccgattatg
     4501 ccgatatcga cgatacctcc ggctggcccg aggtggaaca ggcgcgcagc tcgaaccggg
     4561 tgatcgtgtg ggaacgctat gcccccgatc gctcgccggt gatcaccacc ggctcggcgc
     4621 tgggccatgg cggcgaagtg ctgttcactc gccgcaacgg ccgcgacatc acccagaacg
     4681 tgcgcgatgc gcggcaaacg ctggcgatcg tggtgggcgt ggcgctgctg gtcacggtgt
     4741 tcctctcgct gtttctggcg cgcaccatcg tccagccgct gcgcatgctg gtgcgcgcgg
     4801 cggtgcgcgt gcggctgggc cgcgaccgcg ccgtggtggt gccccgcctg ccccagcgcg
     4861 gcgacgagat cggcctgctc gcccgcgccc tggccgacat gagcgaggcc ctgcgccagc
     4921 ggatcgacgc ggtggaaagc tttgccgccg atgtggcgca tgagatcaag aacccgctgg
     4981 ccagcctgcg cagcgcgctc gacacgctgg acaaggtgga agaccccgag ctgcgccgcc
     5041 agctggccga catcgccgcc catgacgtgc agcggataga ccggctggtg actgaaatcg
     5101 ccgatgccag ccgcatcgat gcggaactgt cgcgcaccac gtttgaactg ctggatatgg
     5161 cggcgctggc ccgcgcattg gtgggcgcgc gggcgatgcg cggggtgaat ggcgggcgca
     5221 agctgcgcgt cttcgcgccc gacgggcagg aggcgccgat cgttgccggt gatccggcgc
     5281 ggctggaacg cgtgctggaa aacctgctcg acaatgccgt ctccttctcg cccgagggcg
     5341 gcacgatcac catcaccatc cgcaccgagg gcgcccaggt ggagctggaa gtgaccgacg
     5401 agggcccggg cattgccgcc gaagcgcgcg agcgggtgtt cgagcggttc cattccctgc
     5461 gcccggccgg cgaggatttc ggccaccatt cggggctggg cctggccatc gcgcgcacca
     5521 tcatcgaggc gcatgacggc acgctggtgg ctgaagaacc cctgcccggc gcccccggcc
     5581 gcccccccgg cgcccggctg gtgatccgcc tgccggccta ttcgccgcgc ggctcgctgc
     5641 tgggatgaac gcggccggga acagccctgc gccccagggc caggcgcatc aggccacagc
     5701 ggtggccatc aacggccgcg cggtgctgat cgaggggccg ccgggcgccg gcaaatccac
     5761 cctggcgctg gcgctgattg atcgcggggc ggtgctggtg ggcgatgatg gcgtgtggct
     5821 gcgggccgag cgcggccggt tgtgggccgc gccgcacccg cgcacgcaag ggctgatcga
     5881 ggtgcgcaac ctcggcatac tggccgcggc cgatctcggc tggccggtct gcccgcaggc
     5941 cccggtggcg ctggtgctgg tgctggacgt ggcggccccc cgcttcatcg aggcgcccga
     6001 ggcgcggcag atcgccggca tcggcctgcc gcagctgcgc ctgtggcccg gcggcgaggg
     6061 cctggcgctg aaggccgaac tggcgctggc ccgccacggg cttgccgcga tttgaccgcg
     6121 gggcgggccc gctgccccga tccggggcgc ccacaaggct gatccccctt cccttttgct
     6181 gcagagcggc gcaaacaggc cccccatgct gcctgattcc gccattgccg cgcccgcccc
     6241 tgccaaaccg ggcgcccaca agcagaaatt gctgctggtg acgggcgttc tgggcgctgg
     6301 caaaagcacg gccctgcgcg cgctggagga tctgggctgg gaagcgatcg acaatttccc
     6361 catccgcctg ctggaacggc tgatcgatgg gcccgatggc aatggggcgc cagccgcggg
     6421 cggcagcgaa tggcccgcgc cgctagcggt gggctttgat tcgcgcacgc gcggcttcaa
     6481 cccgcacacg gtggtggagc tggtaaaaaa gctctcccag cgcgatgatc tggagatttc
     6541 cacgctgttc ctcgattgcg cggtcgatga attgcagcgc cgctataacg agacccgccg
     6601 ccgccacccg ctggccgccg atatgcccgt gagcgccggc atcgccgccg agcgcgagct
     6661 gatggagccc ttgcgccgct gggccgaagt ggtggtgaac accaccgcct tttccaccca
     6721 tcagctcagc caggccatcc gcgagcagtt ttccggcgag gccgggccca atatcaccgt
     6781 caccatcacc agcttcggct ttgcccgcgg catgccgccg gtggccgatt tcgtgttcga
     6841 catgcggttt ctgaacaatc cgcactggca cgaggatctg cgcccccaca ccggccgcga
     6901 ggaaaacgtg gccgccttca tccgccagga tcccgccttc gaggaagcct tcacccgcat
     6961 ccgcgatctg ctgctgctgg tgttgccgcg cttccaggcg cagggcaagg cctatgccca
     7021 catcgccttt ggctgcaccg gcggcaagca ccgctccgtc ttcatggccg aacagattgg
     7081 cgcggcgctg cgcgatgcgg gctttgcgcc caatatcctg caccgcaatc tgggcgcccg
     7141 cgccaccaac ctgatcgaag gcagcccggc gcgatgaccc tttcccgttt ctccggggca
     7201 cagcttgtgc tcctgaccgg tgcccggcgc cggcagcccg cggctgccct gcccaccggc
     7261 cttcgcctaa tggagcgttc ctgacccatg atcggtctta tcctcgtcac ccatggcaag
     7321 cttgccgaag aattcgtgca tgcaatggag catgtcgttg gccggcagga ggcggtggcc
     7381 acggtctgca tcggccctaa cgacgatgtg gagcggcggc ggcgcgagat cgccgatgcc
     7441 atccgcgtgg tcgattccgg ccatggcgcg atcatcctca ccgatctgtt cggcggcacg
     7501 ccctctaacc tggccatctc gctgatggag gcggggcgcg tggaagtgat cgccggcatc
     7561 aacctgccga tgctgatccg cctggcaggc tcacgcaaga cgatggacgt gcgcgccgcc
     7621 gtttcggccg cgcgcgatgc cggccgcaat tacataacag tggcgtccga gttcctcgga
     7681 caagacctat aagcagggag agcagcatgg ccgaggccag gcaaagcgtg gaaatcgtca
     7741 atgccaaggg attgcatgcg cgcgccagcg ccaaattcgt caacatgtgc gccagcctgc
     7801 cggccgatgt ctcggtaacg gtagtgaagg atggcgccga ggctgccggc ggctcgatcc
     7861 tcgggctgat gatgctgggc gcggccaagg gcgacacgat cgagatcagc gtggccgggg
     7921 ccgaggccga caccgtgctg gcccgccttg ccggcctggt gctggatggt ttcggcgagg
     7981 attgatcccc accccatgac gcaaaccgtt cgccgcacga tcaccggctt ttccaatccc
     8041 accgtcaagt tcctgaaatc gctgcgcgac aagaagcacc gccgacgcga gggcaagttc
     8101 ctcgccgaag gcctgcgcct gctgaccgat gcgcgcgaaa gcggccgcct gcccgaaatg
     8161 ctggtgatgg cccgcgggcg cgaggcccat ccgctgctgg ccgaactgga agcggcggtg
     8221 gccgcagccg gcggcgaggt ggtggagatg gaggaggaga tcctcgccaa ggtgacgggg
     8281 aaggacaacc cccaggccgt cgccggcgtc ttcgccgaat tcgacacctc gctcgccggg
     8341 atcgaccggc aggccgcgcc gatctggctg gtggcgcaag ccatgcgcga tccgggcaac
     8401 cttggcacca tgctgcgcac cggcgatgcg gtgggggccg gcggtctcat cctgctcgac
     8461 gattgcgtgg atccgttcag cgtggaagcg gtgcgcgcca gcatgggcgc gatctttacc
     8521 cagcgcctcg ccatggcccg gtgggacgac ttcctgccct ggctgcgggc gggcgcaggg
     8581 cagctggtgg ccgcttcgct gcgtgatgcg gtgccctatc gcggcgcgcc ttatcagggg
     8641 cccgttttca tcatggtggg caatgaatcg cgcgggctgc cggaagccta tgagccggaa
     8701 tgcgacctgc gcgtgacaat gccgatgaag ggccgggccg acagcctgaa tgccgccgtt
     8761 gccgccgccg tgctggccta tgaggtgctg gcgggactgg aggcaggctg aaaaagccct
     8821 gcctccccca gagcctctca gacctcctgc gcctcgggca gggccggcac ctcacccgga
     8881 tcgcctgcgc ccgcatcggc atagcgcggc gcgctttcca ccagcgccac atcctcgatc
     8941 tcgcgcttgc cgatctcgat atgcttgtcg cgcaggcgcc gcccggccga cagcacattg
     9001 cgctcgaaac tgccgacgaa cttgttgtaa ttgtccaccg ccgtggtgag cccgccgccc
     9061 acccgcttca gatgctcggc cgccaccgcc agccggtcat acagctcgcc gcccatccgg
     9121 ccaatctcgc gcgcctcgcg ggccagccca tcctggcgcc acacctgcgc caccgtgcgg
     9181 gcaatcgcca ccagattggt ggggctggcc agcagcacct tgcgctcgaa agcgaagttc
     9241 cagatatcgc ggtcgaattc cagggcagcg gcgatgaaat gctcgcccgg cacgaacatc
     9301 agcacataat cgggcgcttc ctcgaactgg ctctgatagc tcttgctggc cagggtctgg
     9361 atgtgggtgc gcatggacag ggcatgcagg cgcagcgccg cctcgcgcgc ggcctcatcc
     9421 tcggcctcga aggcttcctg ataggcgttg agcgacactt tggcatcgat caccagcttc
     9481 ttcttgccgg ggatgttgac gatggcatcg ggccgcagcc ggccctcctc cgtgtcgatc
     9541 gacttttcgg tgacgaaatc cgtgtgctcg gccaggccgc attgttccag cacgttgcgc
     9601 agctgctgct cgccccagcg gccgcgggcc ttgggggcat tgcgcaggct gttgcccagc
     9661 cgcgcggcct cggccttcac ctgctcctgc cccaggcgca actgctcgat ctggcccagc
     9721 agctggccaa aggcatccac ccggcgcgcc tccagcccct gcacctgctc ctcataggcg
     9781 cgcaggcggt ggctgacagg agcgagcacg gcgcgcaggc gctcctcgct ggtcttctcg
     9841 ctttcggtga agcgggcctc ggcgcgggcc agaaaagcct cctgcgcctt tgccagcacc
     9901 cgcgcgcccg atgcctcgaa ctgcgccttc agcccttcct gggcttccac cagcaggcgc
     9961 ttctgctctt caaaatgggc ggcatgggca cgcagcgtgg ccagttcggc gcgcagcccc
    10021 tcgcgctcgg tggcggcgcg gcgcaattcg ccctccagcc catcggcgcg gctggccctt
    10081 tcgctggcgc cggccagttc ggcgatggcg cggcgaaaac gctcgtccag cgtgcgcagg
    10141 tctgccgcga tggcatcatg gcgcgcccgc cagccggccg cgccccgcgc gcccacaaac
    10201 cagcccagcc cggcaccacc gcccagcccc agcacaacac ccaccaacgc aacagaatcc
    10261 acggcgcact ccagtcagaa cacaccgtga acgctagacg ctgccgcctg ccgtatcaag
    10321 ccgccttgcg ggccttttcc agctttttga gcgccatgtt gcgcttgagg cgcgaaagat
    10381 ggtcgataaa caggatgcct tcgagatggt ccatctcgtg ctggatgcag gtggccatca
    10441 gcccctccat cgccgcctca tgggtggccc catccagatc ctgccagcgc aggcggcagg
    10501 tggccggccg ctccacctcg gcgaagatat ccggcaccga gaggcagccc tcgttataaa
    10561 ccgcataatc ttccgaggga tcgaggattt ccgggttgat gaacacctgc gggttcttct
    10621 tcaccgggcg gtgatcatgg ccgcagccgt cgtcctcgca ggcctcgggg gccgcgtcct
    10681 cgtcgggttc ctgcaggtcg atcaccacca cgcgcaccgc ctcgcccacc tggatggcgg
    10741 caaggccgat gccgggcgca tcatacatcg tctcgaacat gtcggcgaca agcgtcttca
    10801 gcccctcgtc aaacgcggta acgggagcgg acacctgctt gaggcgcgga tcgggtgctt
    10861 ctacaatcgg tcggatagcc atcggccata aatagggcca tgcgccgcga atcgcaacct
    10921 ccccctcagc ccacgcctca ggccagctgc ttcaccccac cgggcggcgc gcgcgcaggg
    10981 cctgggccag cgtgccctcg tcgagatagt ccagctcgcc gcccaccggc aggccatggg
    11041 ccagctgcgt cacccgcacc ggcaggcctt ccagccgctc ggcgatgtag tgggcggtgg
    11101 tctgcccttc cagcgtggcg ttcatcgcca gcaccacctc gtcgataccg ccggcccgca
    11161 cccgctccag cagcgggccg atcgacagat cctccggccg caccccatcc agcgccgaca
    11221 gccgcccgcc cagcacatga tagcgcccgg tgaacaaccg cgaacgatcc agcgcccaca
    11281 gatccgccac atcctccacc acgcacaagg aacgctggtc gcgccgggga tcggtgcaga
    11341 tggcgcaggg gctcaccgtg tccacattgc cgcagatgcg gcattcggcc agccgttcgc
    11401 gcaaggtggc cagcgcctcc agcagctgct ccagcgccgt ctcgcgccgc ttgatcagcc
    11461 acagcaccgc ccgccgcgcg ctgcgcggcc ccagccccgg caggcgggcc agcgatgctg
    11521 ccaatgcctc gatctcttgc gatgccatgc caaggcagat agggccagcc gctgcacagg
    11581 caaagaggcc cgagacaact tatgcgcatc atctacatgg gcacgcccga atttgccgtg
    11641 ccggcgctgg aggcgctggt ggcggcaggg cacgatgtgg tggccgccta ttcgcagccc
    11701 ccccgccccg gcggccgccg
//
LOCUS       DASRYE010000130         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_78105,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000130 DASRYE010000000
VERSION     DASRYE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78105"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFF98_07240"
     CDS             complement(<1..249)
                     /locus_tag="VFF98_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63459.1"
                     /translation="MAAPLSRRAALGLLAAPALVGCGRAGGEEVILTDGGSFWGRAQR
                     RAFYDPFERATGIRVRTVPFAMPGKLRTSIEQGRPIVDV"
     gene            complement(255..1358)
                     /locus_tag="VFF98_07245"
     CDS             complement(255..1358)
                     /locus_tag="VFF98_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU63460.1"
                     /translation="MAVAAPLAILGVSKAFGAAPVLADIHLDVAPGGCLALLGPSGSG
                     KSTLLNLIAGFEQPDSGDIRVGGASVVGLPAHRRAIGMVFQHYALFPHLSVARNIAYP
                     LARRGLARGAQDAAVARLLATVRLERFAHQPVAALSGGQQQRVAIARALAAAPDVLLM
                     DEPMGALDQSLREALQVELKLLLQEAGTTVVYVTHDQREAMALADRLAILNAGRVAQL
                     GATEAVYAAPASAFVAGFLWAGANRLPATARGDAGAGQLQVEACGQLWAARWNPVHPR
                     PLPGTPVALVGRAEDLLVVPQTAVTLAAPLAATVTTSTFAGRGRAVQARLADGTLLAA
                     HQGLEAPAPLPGAAVALAWREGALFAFPAGGEG"
     gene            complement(1391..3499)
                     /locus_tag="VFF98_07250"
     CDS             complement(1391..3499)
                     /locus_tag="VFF98_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63461.1"
                     /translation="MAGGCASAALLVAPAVYAQQAAASPASAQPGDASAPDTKDARPQ
                     DKVIIVNAAKQGHGAPLALSNNPATDPASITVVTQDDIANKVTTSTADLLRGSNGVQV
                     SDYGQVGEAQGVTMRGWSNGYDSSFLSYYQDGMQRNEPSHISSNGYLDLNPLIPETVK
                     QFTIIRGPFDVRYGGNFALAGSIVATTLDTLPNSISVSGGSYGMARALLTLGHSWGDT
                     HLSTAIDGQRSDGYRDSSGAKLLKTFTKFVTPLGGGRFSLGLETYDNQFDQPGYIDEG
                     SIRDGTISAKSASGVNDHGTAHTYSLIGNYLLGDFEKGIEITVGGRRNLLNRTATTIP
                     FPQYYRRDARWEESGSIEGHYHFTLAGVDVLALGGVSIQNSDINLLELPANDGSPIEG
                     INALDAYFYARESITETTKSVYGSLQIKPAEWIKLTAGARYDHFSFDVANQTYDSTNN
                     VYVNAAFKSGTGRFSPKVGLALSPVPWVSFYVNYGQSPHSPSAFGELTSNPGLSVSHL
                     SSVEGGVSFDPWHKRLHFQANFYKTVNTNEVGQVGYTYVNFGTSRRKGYDLEAAVDLL
                     KAGQWGLRLTGNYSHVSARLADGGYVPYVAQWLAAYGIRVQGPVGSKGDTLRLDLDHE
                     FFGPQKLDEAGTFVASPYNRLSAKIRYAIPRLHDLNVWTGAVYYPGSVYNEFAFDLYG
                     RVYTNPEPRVQFQLGAGIKF"
     gene            complement(3707..>4058)
                     /locus_tag="VFF98_07255"
     CDS             complement(3707..>4058)
                     /locus_tag="VFF98_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZU63462.1"
                     /translation="CADLERYLAANDVAAYTKTASHFHAAIIEFSRNAYLIEAVNRSV
                     LMLAGLFRFDLTYPDSMRRDLAEHRQMLAAFDAFDAEAARALMRRHALLDSTVVCDYI
                     AYVEATACDGEGSV"
BASE COUNT          715 a         1354 c         1325 g          664 t
ORIGIN      
        1 gacatcgacg atcgggcggc cctgctcgat gctggtgcgc agcttgcccg gcatggcaaa
       61 gggcaccgtg cgcacgcgga tgccggtggc ccgctcgaag ggatcgtaga aggcgcggcg
      121 ctgggcgcgg ccccagaagc tgccgccatc ggtgaggatc acttcctcgc cgccggcccg
      181 cccgcagcca accagcgcgg gcgcggccag caggccaagg gcggcacggc gcgagagggg
      241 ggcggccatg gcccttaccc ctcgccgcca gccgggaagg caaacagcgc gccctctcgc
      301 caggccagcg ccacagcggc gcccggcagc ggcgcgggtg cctccagccc ctgatgggcg
      361 gccagcagcg tgccatcggc cagccgcgcc tgcaccgccc ggccgcgccc ggcaaaggtg
      421 ctggtggtga ctgtagcggc cagtggggca gccagtgtaa cggccgtctg gggcaccacc
      481 agcagatcct ccgcccggcc caccagtgcc accggcgtgc ccggcaaggg gcgcggatgc
      541 acagggttcc accgtgccgc ccacagctgc ccgcaggcct ccacctgcag ctgcccggcg
      601 ccggcatcgc cgcgcgcggt agccggcagg cggttggcgc ccgcccacag gaagcctgcc
      661 acaaaggcgc tggctggcgc ggcatagacc gcctctgtgg cccccagctg cgccacccgc
      721 ccggcgttga ggatggccag ccgatcggcc agcgccatcg cctcgcgctg gtcatgggtg
      781 acatagacga ccgtcgtgcc cgcttcctgc agcagcagtt tcagctccac ctgcagggcc
      841 tcgcgcaggg actggtcgag cgcgcccatc ggctcgtcca tcagcagcac atcgggcgcg
      901 gcggccagcg cgcgggcaat cgccacgcgc tgctgctgcc cgcccgacag cgcggccacc
      961 ggctgatggg caaaccgctc cagccgcacg gtggccagca ggcgcgccac tgccgcatcc
     1021 tgcgcgccgc gcgccagccc ccggcgcgcc agcggatagg cgatgttgcg cgccacgctg
     1081 aggtggggaa acagcgcata atgctggaac accatgccga tggcgcgccg gtgcgccggc
     1141 aggccgacca cgctggcgcc gcccacgcgg atatcgccac tgtcgggctg ctcgaagccg
     1201 gcgatgaggt tgagcagcgt gctcttgccc gagcccgacg gacccagcag cgccaggcac
     1261 ccgcccggcg ccacatcgag gtggatatcc gccagcaccg gcgctgcgcc aaacgccttg
     1321 cttacgccca gaatggcgag cggggcggct actgccatgc ctgtgccggc ctgcccgcca
     1381 ttccccgcca tcagaacttg atcccggcgc ccagctggaa ctgcacgcgc ggttccggat
     1441 tagtgtagac gcgcccatac agatcgaagg cgaattcatt gtagaccgag ccggggtagt
     1501 agaccgcccc tgtccacaca ttgagatcgt gcagccgcgg gatcgcatag cggatcttgg
     1561 ccgacaggcg gttgtagggg ctggccacga aggtgccggc ctcgtccagc ttctgcgggc
     1621 cgaagaactc gtgatcgaga tcgaggcgca gcgtgtcgcc cttgctgccc accgggccct
     1681 gcacgcggat gccataggcc gccagccatt gcgccacata gggcacatag ccgccatcag
     1741 cgaggcgggc cgaaacatgg ctgtagttgc cggtgaggcg caggccccac tggcccgcct
     1801 tcagcagatc caccgccgct tccagatcat agcccttgcg ccgcgaagtg ccgaaattga
     1861 cataggtgta gcccacctgg ccaacctcgt tggtgttcac cgtcttgtag aaattggcct
     1921 ggaaatgcag ccgcttgtgc cacggatcga aagatacgcc gccttccacg ctggacaggt
     1981 gcgagaccga caggcccggg ttgctggtca gctcgccaaa ggcgctgggc gaatgcgggc
     2041 tctgcccata gttcacatag aacgacaccc atggcaccgg gctgagcgcc aggcccacct
     2101 tggggctgaa ccggccggtg cccgatttga aggccgcgtt cacatagaca ttattggtgg
     2161 aatcataggt ctggttggcc acatcgaagc tgaaatgatc gtagcgcgcg cccgccgtca
     2221 gcttgatcca ctccgccggc tttatctgca gcgagccata gaccgacttg gtcgtctcgg
     2281 tgatgctttc gcgggcatag aaataggcgt cgagcgcgtt gatcccctcg atcggcgagc
     2341 catcattggc gggcagttcc agcaggttga tatcgctgtt ctggatgctg acaccgccca
     2401 gcgccagcac atccacgccc gccagggtga agtgataatg gccctcgatg ctgccgcttt
     2461 cctcccagcg ggcatcgcgg cgataatatt gcgggaaggg aatggtggtg gcggtgcggt
     2521 tgagcaggtt gcgccggccg cccacggtga tctcgatgcc cttctcgaaa tcgcccagca
     2581 gataattgcc gatcagcgaa taggtgtgcg ccgtgccatg atcgttcacg cccgaggccg
     2641 atttggcgct gatcgtgcca tcgcggatag agccctcgtc gatatagccg ggctggtcga
     2701 actggttgtc ataggtctcc agccccaggc tgaagcggcc gccacccagg ggcgtcacga
     2761 atttggtgaa agtcttcagc agcttggccc cgctgctgtc gcgatagcca tcgctgcgct
     2821 ggccatcgat ggcggtagac agatgcgtgt cgccccagct gtggcccagc gtgagcagcg
     2881 cgcgcgccat gccataggag ccgccgctga cgctgatgct gttgggcagc gtgtccagcg
     2941 tggtcgccac gatcgagccg gccagcgcga aattgccgcc atagcgcaca tcgaaggggc
     3001 cgcggatgat ggtgaactgc ttcaccgttt cggggatcag cggattgaga tcgagatagc
     3061 cgttggagga gatatgcgag ggctcgttcc gctgcatgcc gtcctggtaa taggagagga
     3121 aggaggaatc atagccgttc gaccagccgc gcatggtcac cccttgcgcc tcgcccacct
     3181 gaccataatc cgacacctgc acgccattgc tgccgcgcag cagatcggcg gtggaagtgg
     3241 tgaccttgtt ggcgatatca tcctgggtga caacggtgat cgaggcggga tcggtggccg
     3301 ggttgttcga cagggccagc ggcgcgccat ggccctgctt ggccgcgttg acgatgatca
     3361 ccttgtcctg cgggcgggca tccttggtgt cgggcgcgct ggcatcgccg ggctgggcgc
     3421 tggccggcga ggctgcggcc tgctgtgcat acacggccgg cgccaccagc agcgccgcgc
     3481 tggcgcaacc gcctgccaac agtgcccgaa aaacgcgaaa acccccatga atttgattac
     3541 cggatagctc ggtcatctga aaaccctcct gattaggcaa aattcttccg cgcccggaat
     3601 cggatgatta cgggatgtat gcaagaatca ctgctctgta ttcttattgg gtaatctcta
     3661 tcgttttact agataaaaaa tctgtccgac cccggacctc cccttttcac acactcccct
     3721 cgccatcgca ggccgttgcc tcgacataag cgatataatc gcacaccacc gtgctatcga
     3781 gcagggcatg gcggcgcatc agcgcgcgcg ccgcctcggc atcgaacgca tcgaaagcgg
     3841 ccagcatctg ccggtgttcg gccagatcgc ggcgcatgct gtctgggtag gtcagatcga
     3901 aacggaacag gccggccagc atcagcaccg atcggttcac cgcctcgatc agataggcat
     3961 tgcggctgaa ttcgatgatc gcggcgtgga aatgcgaggc cgtcttggtg taggcagcca
     4021 catcattggc ggcgagatag cgttccagat cggcgcaa
//
LOCUS       DASRYE010000131         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_148482,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000131 DASRYE010000000
VERSION     DASRYE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148482"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1421)
                     /gene="murD"
                     /locus_tag="VFF98_07260"
     CDS             complement(57..1421)
                     /gene="murD"
                     /locus_tag="VFF98_07260"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZU63463.1"
                     /translation="MITSSAFAGKRYAVLGLARSGQAALASLVAGGAKVTAWDAREEP
                     RSAVAARFGAAVELADPCAMDLAGYAGVVVSPGVPINRHPIADAARAAGVPLVCDIEL
                     FAQCRASLPPHSVVGITGTNGKSTTTALTTHLLASAGIPALMGGNIGLPILGEAPLPA
                     GGVYVLELSSYQIDLTHSLDCEVAMLLNLSPDHLDRYEGFAGYVASKARLFAMQGVGR
                     LAVVDYRAEMEEDVLRDAPDGQPMLFIDGVSLPGDQADWPSLQGPHNRANAQAAVAAV
                     RHLGVSEAVIEAGLKSFRGLPHRMERVAEAGGVIFINDSKATNPASAAPALAAFPPIG
                     EKRDHKRIHWILGGLPKGDDLDDCAPWFGNVAAAYTIGDAGHRFAEILRPHMPVERAE
                     MMAEAIRRAIAAARPGDVVLLSPACASFDQFRDYEARGAAFRQIVEVLVGAAGEGRSE
                     PGEG"
     gene            complement(1418..2488)
                     /gene="mraY"
                     /locus_tag="VFF98_07265"
     CDS             complement(1418..2488)
                     /gene="mraY"
                     /locus_tag="VFF98_07265"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZU63464.1"
                     /translation="MLYIIAQWLHFPGASNLIRYQTFRAGAALLTALMIGLVIGPRFI
                     TMLRLKQGKGQPIREDGPKTHLAKRGTPTMGGLMILISLVVSMLLWMDLSDPFVWACL
                     AVTVGFGVIGFLDDFDKVTKRSTKGVSGRLRLGAEFLVAGIATWIIVGHTGTNLYVPF
                     LSHVYLPLGLLYYPFAAFVIVGAGNAVNLTDGLDGLATMPVIIAAGAFAIIAYVTGRV
                     DYASYLGIPHVPRAGELAILCAGIMGAGLGFLWFNAPPAAVFMGDTGSLALGGALGAI
                     AVAAHHEIVLAIVGGLFVLEAASVIIQVFVYKRTGKRVFRMAPIHHHFEQKGWAESTV
                     VIRFWIVSIVLALLGLATLKLR"
     gene            complement(2496..>3899)
                     /gene="murF"
                     /locus_tag="VFF98_07270"
     CDS             complement(2496..>3899)
                     /gene="murF"
                     /locus_tag="VFF98_07270"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZU63465.1"
                     /translation="GRVGGGDFTIGNVATDSREITDGGLFFALRGAAMDGHRFVPTAF
                     DHGAAAAIVEQPIDQPHVLVDDSFAALQALARAARERSRATIIGVTGSVGKTSVKEAI
                     HAALDRASRGASHRSVKSYNNHVGVPLSLARLPSRARYGVFEMGMNHAGEIAALTAQV
                     RPHVAVITTIAPAHIENLGSEEAIADAKAEIFAGLEPSDEGAQPVAVIPADSPYAARL
                     RAAAEAQGARVLTFGRAADADVRLLDALAVTGGGSLVTAQLGETRLCYTVAAAGDHWV
                     INSLAVMAAVSAAGADLGAAGLALAELAGLAGRGARHEITCADGGRAVLIDESYNANP
                     ASMRSTLVMLGQTPARRRIAVLGAMKELGDFAPSLHAGLAAPLAEAGVDFALLVGPEM
                     TALAEELQKAGELGKSSPSALGKPIAFAHCSDAQEALAALAEQGLAGGDALLVKGSNS
                     VGLAALVAALLANQPRS"
BASE COUNT          693 a         1373 c         1286 g          547 t
ORIGIN      
        1 gggcggcgtt tgcgccgcgg tggcaaaggg gcgttcctgg gccggatggc tggccattat
       61 ccctctcccg gttcgctgcg gccctccccg gccgcgccca ccagcacctc gacgatctgg
      121 cgaaaggccg cgccccgcgc ctcatagtcg cggaactggt cgaaactggc gcaggcaggg
      181 ctcagcagca ccacatcgcc gggccgcgcc gcggcaatcg cccggcggat cgcctcggcc
      241 atcatctcgg cccgctccac cggcatgtga gggcgcagga tctcggcaaa gcggtggccg
      301 gcatcgccaa tcgtgtaggc ggcggcgaca ttgccgaacc acggcgcgca atcgtccaga
      361 tcatcgccct tgggcaggcc gccaagaatc cagtggatgc gcttgtgatc gcgcttctcg
      421 ccaatgggcg ggaaagcggc cagcgcgggc gcggccgagg ccggattggt ggccttggaa
      481 tcattgatga agatgacccc gccggcttct gccacccgtt ccatccggtg cggcaggccg
      541 cgaaagcttt tgaggccggc ctcgatcacc gcctcgctca cgcccagatg gcgcacggcg
      601 gccaccgcgg cctgggcatt ggcgcggttg tgggggcctt gcaggctggg ccagtcggcc
      661 tgatcgccgg gcaggctcac gccatcgatg aacagcatcg gctggccatc gggggcatcg
      721 cgcagcacat cctcttccat ctcggcgcga taatccacca ccgccagccg gcccacgccc
      781 tgcatggcaa acagccgcgc cttggacgcc acatagccgg caaaaccctc gtagcggtcg
      841 agatggtcgg ggctgaggtt gagcagcatc gccacctcgc aatcgaggct gtgggtgaga
      901 tcgatctgat agctcgacag ttcgagcaca tagacgccgc cggccggcag cggcgcctcg
      961 cccaggatgg gcaggccgat attgccgccc atcagcgccg gaatacccgc cgaggccagg
     1021 agatgggtgg tgagcgcggt cgtcgtgctc ttgccattgg tgccggtgat gcccaccacg
     1081 ctgtgcggcg gcaggctggc gcggcactgg gcaaacagct cgatatcgca gaccagcggc
     1141 acgccggccg cgcgggcggc atcggcgatg gggtggcggt tgatcggcac ccccggcgag
     1201 acgacaacgc ccgcatagcc ggcaagatcc atcgcgcaag ggtcggccag ttccaccgcc
     1261 gcgccaaagc gggcggccac ggccgagcgc ggctcctcgc gcgcgtccca tgcggtcacc
     1321 ttggcgccgc ccgccaccag cgaggccagc gccgcctgcc ccgagcgcgc caggcccagc
     1381 accgcatagc gcttgccggc aaaggcagac gacgtgatca tcgcagcttc agcgtggcca
     1441 ggcccagcag ggccagcacg atggagacga tccagaagcg gatcaccacg gtcgattccg
     1501 cccagccctt ctgctcgaaa tggtggtgga tgggcgccat gcggaacacc cgcttgccgg
     1561 tgcgcttgta aacgaaaacc tggatgatga cgctggccgc ttccagcacg aacaggccgc
     1621 ccacgatcgc cagcacgatc tcgtgatggg ccgccaccgc aatagccccc agtgcgccgc
     1681 ccagcgccag gctgccggta tcgcccatga acaccgccgc cggcggcgcg ttgaaccaca
     1741 ggaagcccag ccccgcgccc atgatgccgg cgcacaggat cgccagctca cccgcgcgcg
     1801 gcacatgggg aatgcccaga tagctggcat agtccacacg gcccgtgaca taggcgatga
     1861 tggcaaaggc ccccgcggca atgatcaccg gcatggtggc caggccatcc agcccgtcgg
     1921 tcaggttcac tgcattgccc gcccccacga tgacgaaagc ggcaaaggga taataaagca
     1981 gccccagcgg cagatagaca tggctgagga agggcacata gaggttggtg ccggtgtggc
     2041 caacgatgat ccaggtggca atgcccgcca ccaggaattc cgcccccagc cgcagccggc
     2101 cggaaacccc cttggtggaa cgcttggtca ccttgtcgaa atcatcgagg aagccgatga
     2161 cgccaaagcc caccgtgacg gcaaggcagg cccagacgaa gggatcggaa agatccatcc
     2221 acagcagcat cgagacgacc agcgagatga ggatcatcag cccgcccatg gtgggcgtgc
     2281 cgcgcttggc cagatgggtc ttggggccat cctcgcggat gggctggccc ttgccctgct
     2341 tcaggcgcag catggtgatg aagcgcgggc cgatgacaag gccgatcatc agcgccgtca
     2401 gcagggcagc gcccgcgcgg aaggtctgat agcggatgag gttgctggcc ccgggaaaat
     2461 gcagccactg ggcgatgata tagagcatgg agctgtcagg accttggctg gttggcaagc
     2521 aatgccgcaa cgagcgctgc cagccccacc gaattggacc ccttgacgag aagcgcatcg
     2581 ccccccgcca ggccctgttc ggcaagcgcc gcaagcgcct cttgcgcatc ggagcaatgg
     2641 gcgaaggcaa tcggcttgcc aagcgcggat ggcgaggatt tccccagctc gcccgctttt
     2701 tgcagctctt cggccagcgc tgtcatctct ggccccacca gcagggcaaa atccaccccc
     2761 gcctcggcca gcggcgcggc aaggccggca tgcaggctgg gggcaaaatc gcccagctcc
     2821 ttcatcgccc ccagcacggc gatgcggcga cgggccggcg tttgccccag catgacaagg
     2881 gtggaacgca tggaggcggg gttggcgttg tagctctcgt cgatcagcac cgcccggccg
     2941 ccatcggcac aggtgatctc gtggcgggca ccgcgcccgg ccagaccggc cagctctgcc
     3001 agggccagcc ccgccgcccc gagatccgcg ccagccgcgc tgaccgccgc catcaccgcc
     3061 agcgaattga tcacccaatg atcgcccgcg gccgccaccg tgtagcacaa acgcgtctcg
     3121 cccagctgcg ctgtcaccag cgagccgccg cctgtcaccg ccagcgcatc gagcaggcgc
     3181 acatcggcat cggccgcccg gccaaaggtg agcacgcgcg ccccttgcgc ctcggccgcc
     3241 gcgcgcagcc ttgcggcata gggcgaatcg gccgggatca ctgccaccgg ctgcgcgccc
     3301 tcatccgaag gctccagccc ggcaaagatt tccgccttgg catcggcgat cgcctcttca
     3361 gagccgaggt tttcgatatg ggccggggcg atggtggtga tcaccgccac atgcgggcgc
     3421 acttgcgctg tcagcgcggc aatctcgccg gcatggttca tgcccatctc gaacacgcca
     3481 taccgcgcgc gcgatggcag ccgcgccagg ctcaagggca cgccgacatg gttgttgtag
     3541 ctcttcaccg agcggtgcga ggcgccccgg ctggccctgt ccagcgcggc gtggatggcc
     3601 tccttcacgc tcgtcttgcc gaccgagccg gtgacgccga tgatggtggc gcgcgatctt
     3661 tcgcgcgcgg cgcgggccag cgcctgcagg gcggcaaaac tgtcgtccac cagcacatgg
     3721 ggctggtcga tcggctgttc gacgatggcc gcggccgcgc catgatcaaa agcggtgggc
     3781 acgaagcggt ggccatccat cgccgccccg cgcagggcga agaacaggcc gccatcggtg
     3841 atctcacgcg aatcggtggc gacattgccg atggtgaaat cacccccgcc aacacgccc
//
LOCUS       DASRYE010000132         6115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_130866,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000132 DASRYE010000000
VERSION     DASRYE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130866"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFF98_07275"
     CDS             complement(<1..558)
                     /locus_tag="VFF98_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HZU63466.1"
                     /translation="MGEEAPVAGERGHGASPQAAAPASVSPGHAQPASALPASPRLPF
                     DEGEDTPASRREQLIEPSRALAIADFAPPRVNAGEWLIRLSYRLGMPASGLVGAFRKP
                     ARVRLLATVESPRPGDRAAGIALRAGHFLIHGLKLPIGQVDFAGAARLTPPAERMVHG
                     FHWLADLSSAAPLDVAAPVAERILAA"
     gene            complement(585..1268)
                     /gene="rpe"
                     /locus_tag="VFF98_07280"
     CDS             complement(585..1268)
                     /gene="rpe"
                     /locus_tag="VFF98_07280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZU63467.1"
                     /translation="MPVPLIAPSILSADFARLGEEVRAIDAAGADWIHVDVMDGHFVP
                     NITIGPAVVKALRPHTQKPFDVHLMISPVDAYLADFAAAGADIITFHPEAGPHAHRTV
                     QAIKALGCKAGVSLNPATPVEVLDYLLEEIDLVLVMSVNPGFGGQKFISSQLRKIEAI
                     RRRIDALGKPIHLEVDGGIDVATLPLALSAGADVLVAGTASFKGGPDHYAGNIAALKA
                     ARPAAGGDG"
     gene            1476..2726
                     /locus_tag="VFF98_07285"
     CDS             1476..2726
                     /locus_tag="VFF98_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZU63468.1"
                     /translation="MASQPLSLAPLPAAARALAEPMVALRRAIHAEPELGLHTPKTLA
                     KLRATLAHLPLEWREGPSTSGAVALLRGGQGAALGALRPTVLLRGDMDALPMQEETGL
                     AFASGVPGHMHACGHDAHTAMLAGAALLLAARADELPGDVLFMFQPGEEGHHGARFML
                     EDGLVDGSCTGAPGRWPGAAFALHVMPNAPHGLVAGRTGALMAGADQIDIAITGAGGH
                     ASMPHHAADPVPVACEIVTALQTMVSRQFDAHDPVVVTIAKISAGTTHNVIPDSAFLK
                     GTMRSLSPASRAALREKASRLARHIAAAHGLEAEVTITPGFPVTVCDREAVELGEEVA
                     GTLFGPASFHRLPAPIMGAEDFAYVLEKVPGAMFFLGAAEEGADWQSACGIHSPRMQL
                     DEAVMPRGAAFLAGLATSWLAAQG"
     gene            2839..3417
                     /locus_tag="VFF98_07290"
     CDS             2839..3417
                     /locus_tag="VFF98_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU63469.1"
                     /translation="MTTRTTSPGLLPAIAERWSPRAFDGSAVPQADLDLILEAAGLAP
                     SAFNYQPWTFLYAHKGDANWEAFLSYLIPFNAGWAKEAGVLAFVVSENDMVQGEKTSP
                     NHSHSFDAGAAWGYAALQAKALGYDTHGMSGIDMDKAIAGLGIPASHRLEAAFVIGKQ
                     GDKAILPDFLQAREAPAPRKPVAAIARAGAFG"
     gene            3617..5623
                     /locus_tag="VFF98_07295"
     CDS             3617..5623
                     /locus_tag="VFF98_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU63470.1"
                     /translation="MVLAFGAAPGAGSPRSSLFRLLAMLAGGAVITLIGAVTYLLLYV
                     SATGRAYEQQIVREAWQQQLAGSQRLLAALVAQPDAGRHLADPADPWRESALAAPLRA
                     ATGARHLALATEPATEPTTRAAPAALVWAGGQPQIVLRAPLAGSGRAVVARIDADAAL
                     LAPFAAAHHLVQPRLLPAGSQPPRLSSVALAGGIVLAWVPADPSRSWQGQLPMILLVV
                     AGLFGGTGFIVLRMSTAITSDLIASEARARHLAFHDTLTGLANRAMMFEKLRSLLAIS
                     RRYYGDIAVHCLDLDRFKEVNDTLGHPAGDELIQQVARRLTGLCRESDTVARLGGDEF
                     VILQPEATSAGASHLAERVLKSFEEPFQLAFGTVEVGVSIGVTVLSNPELDPSEALRQ
                     ADLALYSSKEGGRNRITFFEPDMDAALRLRRGLETDLRHALTHDGLYMVYQPQMDHRG
                     KVAGVEALLRWLHPDKGLIPPAMFVPLAEETGLILDLGEYVFKRVFAETADWLHTRVA
                     INVSPLQLRSPVFMAMLTRLVAEHRIDPSRYEIEITETALLGDDGVTRDNITMLKQEG
                     FSIALDDFGTGYSSLNSLKRFSVDKIKIDRSFVANLEADEEAEALIDAMVKLGRALRL
                     NIVAEGVETEAQRERLAHCGCHQFQGYLMSRPMPASDILRLVGG"
     gene            complement(5627..>6115)
                     /locus_tag="VFF98_07300"
     CDS             complement(5627..>6115)
                     /locus_tag="VFF98_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU63471.1"
                     /translation="RATESHEPFSVVDLDSIVSSVAEDAAEFGAPVVFEGGCGGKARV
                     RPGALVRCLTNLVDNAVKYGGSARLATRVEGGRLMVIVRDGGPGIPEDLLEEVFKPFF
                     RHHGGHAGETGARLPVGDCRAGGTGLGLAIARAQAQALGASLSLANHPDGGLEAQVVL
                     GL"
BASE COUNT          918 a         2230 c         2067 g          900 t
ORIGIN      
        1 ggcggccaga atgcgctctg ccaccggcgc tgccacgtcc agcggcgctg ccgaggaaag
       61 gtctgccagc cagtggaagc catgcaccat gcgttcggca gggggcgtga ggcgggccgc
      121 cccggcaaaa tcaacctggc cgatcggcag cttcagcccg tggatcagga aatggccggc
      181 gcgcagggca atgccggcgg cgcgatcacc cgggcgcggg ctttccaccg tggccaacag
      241 gcgcacccgc gcgggcttgc gaaacgcccc caccagcccc gaggccggca tgcccagccg
      301 ataggacagg cggatcagcc attcgccggc attcacccgc ggcggggcga aatcggcgat
      361 ggccagcgcc cggctgggct cgatcagctg ttcgcggcgg ctggcggggg tgtcctcgcc
      421 ctcgtcaaac ggcaggcgcg ggctggcggg cagggcactg gcaggctggg catggccggg
      481 cgatacactg gcaggcgcgg ctgcctgcgg cgacgccccg tggccccgct cgccggccac
      541 cggcgcttcc tcacccatgc ccaagcccat gccctgccgc atattcagcc gtctccgcct
      601 gccgccggac gggccgcctt cagggccgcg atattgccgg cgtaatggtc tggcccgccc
      661 ttgaagctgg cggtgccggc caccagcaca tcggcgccgg cagacagcgc cagcggcagg
      721 gtggccacgt cgatcccgcc atccacctca aggtggatcg gcttgcccag cgcatcaatc
      781 cggcggcgga tcgcctcgat cttgcgcagc tggctggaga tgaacttctg cccgccaaag
      841 ccggggttca cgctcatcac cagcaccagg tcgatctctt cgaggaggta gtccagcacc
      901 tccaccggcg ttgccgggtt gagcgagaca cccgccttgc agcccagcgc cttgatcgcc
      961 tgcacggtgc ggtgggcatg ggggcccgcc tcggggtgga aggtgatgat atcggcgccg
     1021 gcggcggcaa aatcggccag ataggcatcc accgggctga tcatcagatg cacgtcgaac
     1081 ggcttttgcg tgtgcgggcg cagcgccttc accacggccg ggccaatggt gatgttgggc
     1141 acgaaatggc catccatcac atccacatgg atccagtcgg cgccggccgc gtcgatcgcg
     1201 cgcacttcct cgcccagccg ggcaaaatcg gccgagagga tggatggggc gatcagcggc
     1261 acgggcaggt cggcaggcat gatatggcaa aactcctcaa gccttgcgat tagccagccc
     1321 cgccggcaag gacaaggcgc aagtctgcca ttttccaccg gccattttgc gcgaaacccg
     1381 caagtgcctg cgcaaacttg cgcaaagccc gctcttgccg ccgcctgcca ccgcaaggca
     1441 taaagcaatc ctccccgccg caaaggatgc aagcgatggc cagccagccc ctctccctcg
     1501 cccccctgcc cgccgccgcc cgggcgctgg cagagcccat ggtggcgctg cgccgggcca
     1561 tccatgccga gcccgagctg ggcctgcaca cccccaaaac gctggccaag ctgcgcgcca
     1621 cgctggccca cctgccgctc gaatggcgcg aggggccctc caccagcggc gctgtggcgc
     1681 tgctgcgcgg cggccagggg gcggcgctgg gggccttgcg ccccaccgtg ctgctgcgcg
     1741 gcgacatgga cgcactgccg atgcaggagg aaaccggctt ggccttcgct tccggcgtgc
     1801 ccggccacat gcatgcctgc ggccatgatg cccacaccgc catgctggcg ggcgccgcgc
     1861 tgctgctggc ggcgcgggcg gacgagctgc cgggcgatgt gttgttcatg ttccagcccg
     1921 gcgaggaggg ccaccatggc gcccgcttca tgctggagga tgggctggtg gatggcagct
     1981 gcacgggcgc gccgggccgc tggccggggg cggcctttgc gctgcatgtg atgcccaacg
     2041 cgccgcacgg gctggtggcg gggcgcaccg gcgcgctgat ggccggcgcc gatcagatcg
     2101 acatcgccat caccggcgcc ggcggccatg ccagcatgcc gcatcatgcc gccgatccgg
     2161 tgccggtggc ctgcgagatc gtcaccgcgc tgcagacgat ggtcagccgc cagttcgatg
     2221 cgcatgatcc ggtggtggtc accatcgcca agatcagcgc cggcaccacg cataatgtca
     2281 tccccgacag cgcctttctc aagggcacga tgcgctcgct ctcgccggcc agccgcgcgg
     2341 ccctgcgcga gaaggccagc cgcctggccc gccatatcgc cgccgcccac gggctggagg
     2401 ccgaagtgac gatcaccccc ggcttcccgg tgacggtgtg cgacagggag gcggtggagc
     2461 tgggcgagga agtggccggc accctcttcg gcccggccag cttccaccgc ctgccggcgc
     2521 cgatcatggg cgcggaggat ttcgcctatg tgctggaaaa agtgccgggc gcgatgttct
     2581 tcctgggcgc ggcggaagag ggggcggact ggcaaagcgc ctgcggcatc cattcgcccc
     2641 gcatgcagct ggacgaggcg gtgatgccca ggggcgcggc cttcctcgcc gggctggcga
     2701 caagctggct ggccgcccag ggctgagggc gcccgagggg ataatgaaaa cgtttacgcg
     2761 aaagacttgc gctgcccccg cccgccatgg cagacaccgc cctctctccc gcccgatctg
     2821 cgccgaaagg accccctgat gaccacccgc accaccagcc ccggcctgct gcccgccatt
     2881 gccgagcgct ggagcccgcg cgcctttgat ggcagcgccg tgccccaggc cgacctcgac
     2941 ctcatcctcg aggccgccgg ccttgccccc tcggccttca actaccagcc ctggaccttc
     3001 ctctacgccc acaagggcga tgccaactgg gaggcctttc tctcctatct catccccttc
     3061 aacgccggct gggcgaagga agcgggcgtg ctggccttcg tcgtctcgga aaacgacatg
     3121 gtgcagggcg agaagaccag ccccaaccat tcgcacagct tcgatgcggg cgcggcctgg
     3181 ggctatgccg cgctgcaggc caaggcgctg ggctatgaca cccacggcat gagcggcatc
     3241 gacatggaca aggccatcgc cggccttggc atccccgcca gccaccggct ggaagcggcc
     3301 ttcgtcatcg gcaagcaggg cgacaaggcg atcctgcccg atttcctgca ggcccgcgaa
     3361 gcgcccgccc cccgcaagcc ggtggccgcc atcgcccgcg ccggcgcctt cggctgatcg
     3421 gccctgcccc ggggcgggcc gcggcctgcc ccggcgtgca gatggcccgg cgtgtaggta
     3481 gaaggcacgg gatcggcccc acgcttcggc aagggctggc gcgttatgcc gcgctgcatg
     3541 tcgcgccccg tctcctcgcc gccccgtctc cggcccgccc gcgcacggac tgcccgccat
     3601 gtcggctgag gcgcccttgg tcctggcttt cggcgcggcg cccggggcgg gctcgccgcg
     3661 ctccagcctg tttcgcctgc tggcgatgct ggcgggcggc gcggtgatca cgctgatcgg
     3721 cgcggtcacc tatctgctgc tttatgtcag cgccacgggc cgtgcctacg agcagcagat
     3781 cgtgcgcgag gcctggcagc agcagctcgc cggatcgcag cgcctgctgg ccgccctggt
     3841 ggcccagccc gatgccgggc gccatctggc cgatccggcc gatccctggc gcgagagcgc
     3901 ccttgccgcc cccttgcgcg ccgccaccgg cgcccgccat ctggcgctgg ccacagagcc
     3961 ggccacagag cctaccacac gcgccgcgcc ggccgccctg gtgtgggccg gcgggcagcc
     4021 gcagatcgtg ctgcgcgcgc cgctggccgg cagcgggcgg gcggtggtgg cccgcatcga
     4081 tgccgatgcc gccctgctgg cgccctttgc cgcggcccat catctggtgc agccgcgcct
     4141 gctgccggca ggctcccagc cgccgcgcct ctccagcgtg gcgctggcgg ggggcattgt
     4201 gctggcgtgg gtgccggccg atccctcgcg cagttggcag gggcagttgc cgatgatcct
     4261 gcttgtggtg gccggccttt tcggcggcac cggcttcatc gtgctgcgca tgagcacggc
     4321 gatcaccagc gaccttatcg ccagcgaggc gcgcgcgcgc cacctggcct tccacgacac
     4381 gctgacgggc ctggccaacc gggcgatgat gttcgagaag ctgcggtcgc tgctggcgat
     4441 cagccggcgc tattatggcg acatcgccgt ccattgcctc gatctcgacc ggttcaagga
     4501 agtgaacgac acgctgggcc acccggccgg cgacgagctg atccagcagg tggcccgccg
     4561 cctcaccggc ctgtgccgcg agagcgacac ggtggcgcgc ctgggcggcg acgaattcgt
     4621 catcctccag cccgaggcaa ccagcgcggg cgcctcgcat ctggccgagc gcgtgctcaa
     4681 atccttcgag gagcccttcc agctggcctt tggcacggtg gaagtggggg tgtcgatcgg
     4741 cgtgacggtg ctctccaacc ccgagcttga tccttccgag gcgctgcgcc aggcggatct
     4801 ggcactttac agctcgaagg aaggcgggcg caaccgcatc accttcttcg agcccgacat
     4861 ggatgccgcc ctgcgcctgc gccgcgggct ggaaacagac ctgcgccacg ccctcaccca
     4921 cgacgggctc tacatggtct atcagccgca gatggatcat cgcggcaagg tggccggcgt
     4981 ggaagcgctg ctgcgctggc tgcacccgga caaggggctg atcccgccgg cgatgttcgt
     5041 gccgctggcc gaggagaccg gcctcatcct cgatctgggc gaatatgtgt tcaagcgcgt
     5101 gttcgccgaa accgccgact ggctgcacac gcgcgtggcg atcaatgtct cgccgctgca
     5161 attgcgctcg ccggtgttca tggcgatgct gacccggctg gtggccgaac accgcatcga
     5221 tcccagccgc tacgagatcg agatcaccga gacggcgctg ctgggcgatg atggcgtgac
     5281 ccgcgacaac atcaccatgc tgaagcagga gggcttttcc atcgccctcg acgatttcgg
     5341 caccggctat tccagcctca attcgctgaa gcgcttctcg gtcgacaaga tcaagatcga
     5401 ccgttccttc gtcgccaatc tggaggccga cgaggaagcc gaggcgctga tcgacgcgat
     5461 ggtgaagctg ggccgggcgc tcaggctcaa catcgtggcc gaaggggtgg aaaccgaggc
     5521 ccagcgcgaa cgcctcgccc attgcggctg ccaccagttc cagggctatc tgatgagccg
     5581 gccgatgccc gcatccgata tcctgcggct ggtcggcggg taaagatcaa agccccagca
     5641 ccacctgcgc ttccagcccg ccatcggggt ggttggcaag gctgagcgag gcgcccagcg
     5701 cctgggcctg ggcgcgggca atggccagcc ccagcccggt gccgccggcc cggcaatcgc
     5761 ccaccggcag gcgcgcgccg gtttcgccgg catgcccgcc atggtggcgg aagaagggct
     5821 tgaacacctc ctccagcaga tcctcgggga tgcccgggcc gccatcgcgc acgatcacca
     5881 tcagccggcc gccttccacc cgggtggcca gccgggcgct gccgccatat ttcaccgcat
     5941 tgtccaccag attggtcagg caacgcacca gcgcgcccgg ccgcacccgc gccttgcccc
     6001 cgcagccacc ctcgaacacc accggcgcgc cgaattcggc ggcatcctcg gccacgctgg
     6061 agacgatcga atcgagatcg accaccgaga acggctcgtg gctttcggtg gcgcg
//
LOCUS       DASRYE010000133         7822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_23055,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000133 DASRYE010000000
VERSION     DASRYE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23055"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFF98_07305"
     CDS             complement(<1..792)
                     /locus_tag="VFF98_07305"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZU63472.1"
                     /translation="MASRPETDSSLAGTVATTVLVVPAMHCAGCIGKVEQGLGAMPGV
                     ASARVNLSARQVSVVHEAAVGVPAMLQVLSGLGYAAQPRDEDLAPPPSAVKPLLAPLA
                     VAGFACMNVMLLSVSIWSGAAGATRDLFHWLSALIGVPAIAYAGRVFFDSAWGALSRG
                     RTNMDVPISIGVLLATGLSFYETLTHGAEAWFDGTLMLLLFLLAGRALDAAMRDRARA
                     GVDALLRQAASGAMVVGGDGALEWVAARDLAGGQVMRVAAGERLAA"
     gene            complement(792..1268)
                     /locus_tag="VFF98_07310"
     CDS             complement(792..1268)
                     /locus_tag="VFF98_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HZU63473.1"
                     /translation="MTALSAPAPRRRFRFTGWHMLAILVGFFGIVIAVNVYMAMLASE
                     TFSGVVVENSYVASQHYNHWLDEAAKERALGWNATVSRQIDGHLAVSITGAGSENATL
                     TAEAWHPLGQLADRNLTFRKLATGSYVSNEALPEGRWRLRLALNAGGHVWRTNERL"
     gene            complement(1309..2850)
                     /gene="ccoG"
                     /locus_tag="VFF98_07315"
     CDS             complement(1309..2850)
                     /gene="ccoG"
                     /locus_tag="VFF98_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HZU63474.1"
                     /translation="MGSLEPSGQPQASQPGTSPAGKAGGKAGSKAGRGGGAQGAAPSG
                     LHAASKAVYPRQIDGRFRRLKWAIMAFCLAVYYITPWLRWDRGPFSPHQAVLIDLAHR
                     RFYIFAIEIWPQEFYFVAGLLIMAGIGLFLVTSAVGRAWCGYACPQTVWTDLFQHVDR
                     LIDGDRNAQLKLAKAPWGLAKIGKRLFKWSVYLAISFSTGGAWILYFDNAPTLFHDFF
                     TGTAPGIAYSTVGVLTGTTLWLGGFMREQVCVYMCPWPRIQSAMLDEKSLIVTYKDWR
                     GEPRGKPAKNSTVPMGDCVDCDLCNAVCPTGIDIREGPQIGCITCALCIDACDSVMTK
                     LGRPRGLIDYATLEDTERERTGQPATPHWKLVWHARTLVYLGVWSAIGAGLLFALGIR
                     HHLAISVAKDRNPPFMLMSDGDVRNAYLVKWRNMEDRPRHMVLELGGLPGGRMWSEDM
                     GKAEAARSLARTVAADQTEPLRVYVVAPSGTAEQSFSFTLRALDAEGGQVAYTARFDA
                     PAAQP"
     gene            complement(2837..3781)
                     /gene="ccoP"
                     /locus_tag="VFF98_07320"
     CDS             complement(2837..3781)
                     /gene="ccoP"
                     /locus_tag="VFF98_07320"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HZU63475.1"
                     /translation="MSEKRIDEATNTETMGHEWDGIEELNTPLPTWWLWTFYACILFA
                     IGYCVVYPAIPGLHKGSEGTAGWTSRGSFAKEMDAAALARKPVLDALAATPIEDLPKK
                     PELMAAAVAGGRAAFKVNCVQCHGAGAAGGVGYPNLNDDDWLWGGTLTDIQQTLTHGI
                     RYPGDEQTRMSQMPAFGRDGILQAAQVQDVVSFVRSLSGLEPKGAAAQRGAALFAANC
                     AVCHGPEGKGGRMVGAPNLTDKIWLYGSSREAVTNTVTNAHAGVMPAWKDRLDPVTIK
                     MLAAYVHSLGGGEAFAPAAAPAAAAPAPAASGAANGQP"
     gene            complement(3774..3944)
                     /locus_tag="VFF98_07325"
     CDS             complement(3774..3944)
                     /locus_tag="VFF98_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HZU63476.1"
                     /translation="MSEPHTYETLRQLADSWGLGIMTVLFLGLCLWPFRPGGRKDSDH
                     AAHMIFEDDDNV"
     gene            complement(3941..4702)
                     /gene="ccoO"
                     /locus_tag="VFF98_07330"
     CDS             complement(3941..4702)
                     /gene="ccoO"
                     /locus_tag="VFF98_07330"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HZU63477.1"
                     /translation="MSSETKQPFAWHSTLERNITILGICAFIAVIFGGIVEIGPLFWL
                     DNTIEKVKGMRPYTPLEQAGRDIYIREGCYLCHSQMIRPFRDETERYGHYSLAAESMY
                     DHPFQWGSERTGPDLARVGGKYSDAWHVQHLSAPRSVVPESIMPNYAFLANTELKAGD
                     MRAELATLAKVGVPYTAEDIAKANDDLKLQANANGSASELDKRYAKAQVRDFDGKPDK
                     ITEMDALVAYLQVLGTMVDVESAAAQEQLAQEKGR"
     gene            complement(4716..6374)
                     /gene="ccoN"
                     /locus_tag="VFF98_07335"
     CDS             complement(4716..6374)
                     /gene="ccoN"
                     /locus_tag="VFF98_07335"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HZU63478.1"
                     /translation="MGTVVTRAGIWLALLLASFIAMVAAKDTPFAIQMGTVAAATLVG
                     LWFSISKVDYGAIAKGFIRSPADHGRYDDEVIRWGVIATVFWGMAGFLVGLIIALQLA
                     FPDLNVGPWFNFGRMRPLHTSAVIFAFGGNALIATSFYVVQRTCRARLALPGVARFVF
                     WGYQLFIVLAATGYLLGITQGKEYAEPEWYVDLWLTVVWVAYTAVFIATILKRSEPHI
                     YVANWFYLSFLITVAMLHIVNNLAIPVSFLGSRSISFFGGVQGAMVQWWYGHNAVGFF
                     LTAGFLGMMYYFVPKQAQRPVYSYRLSIIHFWALIFLYIWAGPHHLHYTALPDWAQTL
                     GMVFSVMLWMPSWGGMINGLMTLNGAWDKLRTDPVLRMFVMSLAFYGMSTFEGPMMSV
                     KSVNSLSHYTNWTIGHVHSGALGWNGMVTFGALYYLTPRLWNRERMYSLRLVNWHFWT
                     ATLGIVFYAASMWVAGITQGLMWREYGADGYLVNSFADAVAAIHPMYVLRAFGGLLYL
                     SGACVMTYNIWRTIAVGDLRDEAPLHNIDQHLDRDHPVVAAAAE"
     gene            6731..7450
                     /locus_tag="VFF98_07340"
     CDS             6731..7450
                     /locus_tag="VFF98_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZU63479.1"
                     /translation="MRKISRAITILASLGAAAGFAGAAHADDYTGHFQVKVLATGVLP
                     SGHIDSIKGDPLGLTKSEGALYGLQTQANNNVVPTLAVEYFLKPNISFETICCVTGHH
                     VSASANSGAVPGVNLVDHITILPATLTVKYHLVGMLPAGIKPYVGVGPAVFIMLSDRP
                     SETVKSALGVTRTKLSSNVGAVVQAGVDVPVGHGYGISLDAKKYFVGTTAHFYAGSVD
                     ALDADVKLDPWVISAGVSYRF"
     gene            complement(7659..>7822)
                     /locus_tag="VFF98_07345"
     CDS             complement(7659..>7822)
                     /locus_tag="VFF98_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU63480.1"
                     /translation="LPFSRQQIADVLGLTIETVSRQFTRLKGDGVIDLPSRREVVIVD
                     RGALMAEAG"
BASE COUNT         1432 a         2590 c         2546 g         1254 t
ORIGIN      
        1 ggcggccagc ctctcgccgg cggccacccg catcacctgg ccgcccgcca gatcgcgcgc
       61 ggccacccat tccagcgcgc catcgccgcc caccaccatc gccccgctgg ccgcctggcg
      121 cagcagcgca tcaacaccgg cgcgcgcccg gtcacgcatg gcggcatcca gcgcccggcc
      181 cgccagcagg aacagcagca gcatcagcgt gccatcaaac cacgcctcgg cgccatgggt
      241 cagcgtttca taaaagctga ggccagtggc cagcagcacg ccgatcgaga tcggcacatc
      301 catgttggtg cggccacgac tgagcgcacc ccaggccgaa tcgaagaaca cccggccggc
      361 ataggcgatc gccggcacgc cgatcagcgc cgacagccag tggaacagat cacgcgtggc
      421 gcccgccgcg cccgaccaga tgctgaccga cagcagcatc acattcatgc aggcaaaacc
      481 ggccaccgcc agcggcgcca gcagcggctt caccgccgat ggcggcggcg ccagatcctc
      541 gtcgcgcggc tgggcggcat agccaaggcc cgagagcacc tgcagcatcg ccggcacgcc
      601 caccgccgct tcatgcacca cgctcacctg ccgggccgac aggttcaccc gcgccgaggc
      661 cacccccggc atcgccccca gaccctgctc caccttgccg atgcagccgg cgcaatgcat
      721 cgccggcacc accagcacgg tggtagccac cgtgccggca agggaactgt ccgtctcggg
      781 gcggctggcc atcacaggcg ctcgttcgtg cgccagacgt ggccgccggc gttcagcgcc
      841 agacgcagcc gccagcgccc ctcgggcagc gcctcattcg agacatagct gcccgtggcc
      901 agcttgcgga aggtgaggtt gcgatccgcc agctggccca gcgggtgcca ggcctcggcg
      961 gtcagcgtgg cgttctcgct gccagcgccg gtgatgctga ccgccagatg cccgtcgatc
     1021 tgccgcgaca ccgtggcatt ccagcccagc gcgcgttcct tggcggcctc gtccagccag
     1081 tggttgtagt gctggctggc gacataggaa ttttccacca ccacgccgct gaacgtctcg
     1141 ctggcgagca tcgccatata gacgttcacc gcgatgacga tgccgaagaa gcccaccagt
     1201 atcgccagca tgtgccagcc ggtgaagcgg aagcgacggc gcggggcggg agcggaaagg
     1261 gcggtcatcg gaagtctcct agcaggaggg atatggggcg agaggcgatc agggctgggc
     1321 ggcaggggcg tcgaagcggg cagtgtaggc cacctggccg ccctcggcat ccagcgcgcg
     1381 cagggtgaag ctgaaggatt gctcggccgt gcccgaaggc gccaccacat agacgcgcaa
     1441 gggctcggtc tggtctgcgg ccacggtgcg cgcaaggctg cgcgcggcct cggccttgcc
     1501 catgtcctcg ctccacatgc gcccgccggg caggccgccc agttccagca ccatgtggcg
     1561 cggccggtct tccatgttgc gccacttcac cagataggca ttgcgcacat cgccatcgct
     1621 catcagcatg aagggcggat tgcgatcctt ggccacggaa atggccagat ggtggcggat
     1681 gcccagggca aacagcagcc ccgcgccaat cgccgaccac acgcccagat agaccagcgt
     1741 gcgcgcatgc cagaccagct tccagtgggg cgtggccggc tggccggtgc gctcgcgctc
     1801 ggtatcttcc agcgtggcat aatcgatcag cccgcgcggg cggcccagct tggtcatcac
     1861 gctgtcgcag gcatcgatgc acagcgcgca ggtgatgcag ccgatctgcg gcccctcgcg
     1921 gatgtcgatc ccggtggggc acacggcatt gcacaggtcg caatcgacgc aatcgcccat
     1981 cggcacggtg ctgttcttgg ccggcttgcc gcgcggctcg ccccgccagt ccttgtaggt
     2041 gacgatcagc gatttctcgt cgagcatggc cgactggata cgcggccagg ggcacatgta
     2101 gacgcacacc tgctcgcgca tgaagccgcc cagccacaag gtggtgccgg tgagcacacc
     2161 cacggtggaa taggcgatgc cgggggccgt gccggtgaag aaatcgtgga acagggtggg
     2221 cgcattgtcg aaatagagga tccaggcgcc gccagtggaa aagctgatgg ccagatagac
     2281 cgaccatttg aacagccgct tgccgatctt ggccagcccc cagggggcct tggccagctt
     2341 cagctgggcg ttgcgatcgc catcgatcag ccggtcgaca tgctggaaca ggtctgtcca
     2401 caccgtctgc gggcaggcat agccgcacca ggcgcgcccc actgccgaag tgacgagaaa
     2461 caggccaatg ccggccatga tgagcaggcc cgcgacgaag tagaactcct gcggccagat
     2521 ctcgatggcg aagatgtaga agcggcggtg ggccaggtcg atcagcaccg cctgatgcgg
     2581 cgagaagggc ccgcgatccc agcgcagcca gggggtgatg tagtataccg ccaggcagaa
     2641 ggccatgatg gcccacttca gccgacggaa gcgcccatcg atctggcggg gatagaccgc
     2701 cttgctggcc gcgtggaggc cggatggggc agcgccttgc gcgccgcccc ccctgcccgc
     2761 cttgctaccc gccttgccac ctgccttgcc ggccgggctg gtgcccggct ggctggcttg
     2821 cggctggccc gaaggctcaa ggctgcccat tggccgctcc gctggccgca ggggcgggcg
     2881 ctgctgctgc aggcgccgcc gccggggcaa aggcctcgcc accgccaagg ctgtggacat
     2941 aggccgccag catcttgatc gtcaccggat cgaggcgatc cttccaggcc ggcatcacgc
     3001 ccgcatgggc attggtgacg gtgttggtga cggcctcgcg gctggaacca tagagccaga
     3061 tcttgtcggt gaggttgggc gcgccgacca tgcggccgcc cttgccttcc ggcccgtggc
     3121 acacggcgca attggccgcg aagagggcag cgccccgctg agcggcagcg cccttgggct
     3181 ccagccccga aaggctgcgc acaaagctca ccacatcctg cacctgggcg gcctgcagaa
     3241 tgccatcgcg gccaaaggcc ggcatctggc tcatgcgggt ctgctcgtcg cccggatagc
     3301 ggataccatg ggtcagcgtc tgctggatgt cggtcagcgt gccgccccac agccagtcgt
     3361 cgtcgttgag gttgggatag ccaacgccgc cggccgcacc cgcgccatgg cactgcacgc
     3421 agttcacctt gaaggccgcg cggccaccgg ccacggccgc cgccatcagc tcaggcttct
     3481 tgggcagatc ctcgatcggc gtggcggcca gcgcatccag cacgggcttc ctggccagcg
     3541 ccgcggcatc catctccttg gcgaaggagc cgcggctggt ccagccggcg gtgccctcag
     3601 accccttgtg cagccccgga atggccggat agaccacgca ataaccgatg gcaaagagga
     3661 tgcaggcata gaaggtccac agccaccagg tcggcagcgg cgtgttcagt tcctcgatgc
     3721 cgtcccactc atggcccatg gtctcggtgt tggtcgcttc atcgatgcgt ttctcagaca
     3781 ttgtcgtcgt cctcgaagat catgtgggcg gcatggtcgg aatccttgcg gccgccgggg
     3841 cggaacggcc acagacacag gcccaggaac aggaccgtca tgatgcccag cccccagctg
     3901 tcggccagct ggcgcagggt ttcgtaggtg tgtggttcgc tcaccgcccc ttctcctgtg
     3961 ccagctgttc ctgcgctgcg gcgctttcca catcgaccat cgtgcccagc acctgcagat
     4021 aggccaccag tgcgtccatc tcggtgatct tgtcgggctt gccgtcgaaa tcgcggacct
     4081 gggccttggc gtaacgcttg tcgagctcgc tggccgagcc attggcattg gcctgcagct
     4141 tgaggtcgtc attcgccttg gcgatgtcct cggcggtgta gggcacgccc accttggcca
     4201 gcgttgccag ttctgcgcgc atgtcgcccg ccttcagctc ggtgttcgcg aggaaggcat
     4261 agttgggcat gatcgattcg ggcaccacgc tgcgcggcgc ggaaagatgc tggacatgcc
     4321 aggcatccga atatttgccg cccacgcggg ccagatccgg cccggtgcgc tccgaacccc
     4381 actggaaggg gtgatcatac atcgattcgg cggccaggct gtagtggcca taacgctccg
     4441 tctcatcgcg gaaggggcgg atcatctggc tgtggcacag atagcaccct tcgcggatgt
     4501 agatgtcgcg gccggcctgt tcgagcgggg tgtaggggcg catgcccttc accttctcga
     4561 tcgtattgtc gagccagaac agcggcccga tttccacgat cccgccgaag atcaccgcga
     4621 tgaaggcgca gatccccagg atggtgatgt tgcgttccag cgtggagtgc caggcgaagg
     4681 gttgtttcgt ttcagaagac atggcttggc tcccctcact cggccgcggc agcaaccacc
     4741 gggtggtcgc gatcgaggtg ctggtcgatg ttgtggagcg gcgcctcgtc gcgcagatcg
     4801 cccacggcaa tggtgcgcca gatgttgtag gtcatcacac aggcgcccga gaggtagagc
     4861 agcccgccaa aggcgcgcag cacatacatc gggtggatcg cggccaccgc atcggcaaag
     4921 ctgttcacca gatagccatc ggcgccatat tcacgccaca tcagcccctg cgtgatgccc
     4981 gccacccaca tgctggcggc atagaacacg atgcccagcg ttgcggtcca gaagtgccag
     5041 ttgacgaggc gcagcgaata catccgctcg cggttccaca ggcgcggggt gagatagtaa
     5101 agcgcgccga aggtgaccat gccgttccag cccagcgcac cggaatgcac gtggccgatc
     5161 gtccagttgg tgtagtgcga gagcgagttc acgctcttca cgctcatcat cgggccctcg
     5221 aaggtgctca tgccatagaa ggcaaggctc atcacgaaca tgcgcagcac agggtctgtg
     5281 cgcagcttgt cccaggcgcc gttcagcgtc atcaggccgt tgatcatgcc gccccagctg
     5341 ggcatccaca gcatcaccga gaacaccatg cccagggtct gcgcccagtc gggcagggcg
     5401 gtgtagtgca ggtggtgcgg gccggcccag atatacagga agatcagcgc ccagaagtgg
     5461 atgatcgaca ggcgatagga atagaccgga cgctgggcct gcttgggcac gaaataatac
     5521 atcatgccca ggaagcccgc ggtcaggaag aagcccaccg cattatggcc ataccaccac
     5581 tgcaccatcg ccccctgcac gccgccgaag aagctgatcg agcggctgcc caggaagctg
     5641 acggggatgg cgaggttgtt gacgatgtgc agcatcgcca cggtgatcag gaacgagagg
     5701 tagaaccagt tggccacata gatatgcggc tcggagcgct tgaggatggt ggcgatgaac
     5761 accgccgtat aggccaccca cacaaccgtc agccacaggt cgacatacca ttccggctcg
     5821 gcatattcct tgccctgggt gatgccgagc agatagcccg ttgccgccag cacgataaac
     5881 agctgatagc cccagaacac gaaacgcgcc acgcccggca gcgccaggcg cgcccggcag
     5941 gtgcgctgca ccacatagaa gctggtggcg atcagcgcgt tgccgccaaa ggcgaagatc
     6001 accgcgctgg tgtgcagcgg gcgcatgcgg ccaaagttga accagggccc aacgttgaga
     6061 tccgggaatg ccagctgcag ggcgatgatc aggcccacca ggaagccggc catgccccag
     6121 aagacagtgg cgatcacgcc ccagcgaatc acctcgtcgt catagcggcc atgatcggcc
     6181 ggcgaacgga tgaaaccctt ggcgatggcg ccataatcga ccttgctgat cgaaaaccac
     6241 aggcccacca gcgtggctgc ggcaaccgtt cccatctgaa tggcgaacgg ggtgtccttg
     6301 gcggccacca tggcgatgaa tgaagcgagc aatagtgcaa gccatatgcc ggcccgcgtg
     6361 acgactgttc ccatgagcct tccctctcat gcatgatgca tttggttgcg cctatgctgg
     6421 gcaggcaggc agggcacatt gatccccgtc aaaccctcca gctcttggcc ttccagcccc
     6481 tggccttccc ggtcagacgg cccccgactt aagtattaga actaggcggc tgatcttacg
     6541 gatgtattgc aggaatacaa cttgtctcaa aggatggcgc gggcaggcta tcatggagtt
     6601 gcgcaggatc aaataagggc ttgcgcatga tcaatgctgc ccgggcgcgg ctgcgccaaa
     6661 gagcatccgt cgccgcgatg atgaggcaag gcctgcacag ggcgggcata gcggcaatga
     6721 ggaaataacc atgcgcaaga tttccagggc gatcaccatt ctggccagcc tgggcgccgc
     6781 cgccggcttc gcgggcgcag cccatgccga cgattacact ggccacttcc aggtgaaggt
     6841 gctggccacc ggcgtgctgc cgtcgggcca tatcgacagc atcaagggcg atccgctcgg
     6901 cctcaccaag tcggaaggcg cgctgtatgg cctgcagacc caggccaaca acaatgtcgt
     6961 gccgaccctg gccgtggaat atttcctcaa gcccaacatc tcgttcgaga ccatctgctg
     7021 cgtcaccggc caccatgtgt cggcctcggc caactcgggc gcggtgccgg gcgtgaacct
     7081 ggttgatcac atcaccatcc tgcccgccac gctgacggtg aagtatcact tggtgggcat
     7141 gctgccggcg ggcatcaagc cttatgtggg cgtgggcccg gccgtgttca tcatgctgtc
     7201 cgaccggccg agcgagaccg tgaagtcggc gctgggtgtc acccgcacca agctttcgag
     7261 caatgtgggc gcagtggtgc aggccggtgt cgatgtgccg gtgggccatg gctatggcat
     7321 cagcctggat gccaagaagt atttcgtggg caccaccgcg catttctatg ctggctcggt
     7381 cgatgctctc gatgccgacg tgaagctcga tccctgggtg atcagcgccg gcgtgagcta
     7441 ccgcttctga ggcacaaagg gccatgcgct ccacagctgg ggcggcttat gaaagcgcat
     7501 ggcctgcctt ggcaggcggc aagcgggcgg cagggcgtgg gaaggcgcac tgtcgcccgc
     7561 acctttttgg gggcgcggtt tttggtgggc acggcaacgg gccgcaagcg gcgccgggat
     7621 agcggtgaaa ggggatggca ggccggcaga aggccggttc agcccgcctc ggccatcagc
     7681 gcgccgcgat cgacgatcac cacttcgcgg cggctgggca gatcgatcac gccatcaccc
     7741 ttcagccggg tgaactggcg gctcacggtc tcgatcgtca ggcccagcac atcggcgatc
     7801 tgctggcggc tgaacggcag gg
//
LOCUS       DASRYE010000134         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_163186,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000134 DASRYE010000000
VERSION     DASRYE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163186"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFF98_07350"
     CDS             <1..1108
                     /locus_tag="VFF98_07350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU63481.1"
                     /translation="ALATHTSHIGLIATVSTTYLAPYHVARKFASLDALSGGRAGWNL
                     VTSGTDAEAANFGLPHQLTHDRRYAVAAEHVEVVKALWDSWDENAVIADKATGRFFDP
                     ARVHPLNHEGAHFSVRGPLQTTRPPQGHPVIVQAGSSEDGQRLAAATAELVFTAQQDR
                     ASARAFTQGLKDRAEALGRARDDILVLPGVSIYVGATREEAQAKLDVLQQFVDPVASV
                     QAFKVFLDWDLTGVDLDSPPTEPPFTEGWQSRQRLLYDLAVREGLSVRQLVGRLSAAR
                     GHLVLVGTPTDIVDELQAWFEEGAADGFNILAPIFPQGLDDVIEQVLPELRRRGLFRS
                     EYEGTTLRANLGLKRPAARREAQPAQPTAPALAV"
     gene            1120..2559
                     /locus_tag="VFF98_07355"
     CDS             1120..2559
                     /locus_tag="VFF98_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63482.1"
                     /translation="MASLADNLGAAPAGGPAPHRAGQVSGWRLALFSSQSMALAAVEV
                     PLSTYVPPLYASAFGFSLTTVGLIFLAARLWDAVIDPGIGILSDRTRSRWGRRRPWIA
                     LGGAIFAVGAVPVFLPPAWFGPAALSAALFTLYLGYSMIATPFAAWSGELSALYHERT
                     RVATYAQGMTSVALLLALVLPSLLVRQFEHQPRLQLAAMGAMVFALLIATLPLGLRAL
                     PEPAPEAGPLPPLRLGESLKVVFTERLLPRVLASNFAVRLGQGVRTALFVFFVTHYLG
                     RPSWAPGLFLLQYVFGVFACPIWLAVGRRLGKARAAVAGELVQVAINLALLGLAHDSI
                     ALLVGLTVAQGLAQGSGNLMLRSIVADVADNHRLWSGEDRIGLFYSVFSLSDKAAFAV
                     AVGVALPLAAWLGFDPKHANSAAVLMHLKWVFALGPALAHLFSAALIRGFPLNEERHG
                     EIRRALAARDAAGLISDASRERWLAEADD"
     gene            complement(2612..>4587)
                     /locus_tag="VFF98_07360"
     CDS             complement(2612..>4587)
                     /locus_tag="VFF98_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HZU63483.1"
                     /translation="PMRIFRQCLLLATAALCVPASAQTPPADPPAFDPPAFDPPSART
                     LAAQAAAAASAPFADRRDEDFASRGFVATRADPLIRAADGHVVWNLDAYAFVKGPAPA
                     TVNPSLWYHTGLLAKHGLFRVSPRIWQVRGFDVANITFVRGEKGWVLIDTLTTTETAR
                     AAYELVSQQLGKWPITGIIYTHSHVDHFGGAGAFAADLAPGAPIIAPAGFRAAAVSEN
                     IIAGPAMQRRASYQFGVPLAAGPTGSLGSGIGLGAALGSQGLIAPTREISQTGTEITI
                     DGVHIRFQLTPGTEAPAEMNLGFPDWQVADLAENANVTQHNILTPRGAVIRNAHAWAQ
                     GLTEAMDFFAGAEVLITSHGWPRWGAGEITDYLGKHRDAYAFLHDQTVRWMNKGLRAD
                     EIAAKISLPAALQKEWYDRPYYGSLSFNARAVYQYYLGWYDANPVHLAPLPPAEGGQR
                     YVEALGGAARVRALAQAAYDKGDYAWAAELLNRAVFADPADRAARALLARAYQQLAWA
                     SENSVWRNIYLTGAQELLGGVKVTNTAAKAGIAASLPPDDLFDVLATRLDAEKVGDQA
                     LRLGFVFPDRGESWTVVIAHGVLTHHKGIIGQPDVTLNVNYPDLLAAQTGGEPLAAKI
                     MAGEASVTGDLLALRRFGGWIDKPDPAFPIVTP"
BASE COUNT          659 a         1551 c         1609 g          768 t
ORIGIN      
        1 agcgctcgcc acgcacacca gccatatcgg cctcatcgcc acggtatcga cgacctatct
       61 tgcgccttat cacgtggcgc gcaaattcgc ctcgctcgat gcgctgtcgg gcgggcgggc
      121 gggctggaac ctggtgacat cgggcaccga tgcggaagcg gccaatttcg gcctgccgca
      181 tcagctcacc cacgaccgac gctatgcggt tgccgccgaa catgtcgagg tggtcaaggc
      241 cctgtgggac agctgggacg agaatgcggt gatcgccgac aaggcaacgg gccgcttctt
      301 cgatccggcc agggtgcatc cgctcaacca tgaaggcgcc catttcagcg tgcgcggccc
      361 cttgcaaaca acccgcccgc cccagggcca cccggtgatc gtgcaggccg gctcctcgga
      421 agacgggcag cgcctggccg ccgccaccgc cgagctggtg ttcaccgccc agcaggaccg
      481 cgccagcgcg cgcgccttca cccagggcct gaaagaccgc gccgaggcgc tgggccgcgc
      541 ccgcgacgat attctggtgc tgcccggcgt gtcgatctat gtcggcgcca cgcgtgagga
      601 agcgcaggcc aagctggatg tgctgcagca gtttgtcgat ccggtcgcca gcgtgcaggc
      661 cttcaaagtg ttcctcgact gggatctgac cggcgtcgat ctcgacagcc cgcccaccga
      721 accgcccttc accgagggct ggcagagccg ccagcgcctg ctctacgatc tggccgtgcg
      781 cgaggggctc agcgtgcgcc agctggtggg gcggctgtcg gcggcgcgtg gccatctggt
      841 gctggtgggc acgcccaccg acatcgtcga cgagctccag gcctggttcg aggaaggcgc
      901 ggccgatggc ttcaacattc tggcgccgat cttcccgcag gggctcgacg atgtcatcga
      961 gcaggtgctg cccgaattgc gccgccgcgg gctgttccgc agcgaatatg aaggcacgac
     1021 cctgcgcgcc aacctcgggc tgaagcgccc ggcggcccgg cgcgaggccc agcccgccca
     1081 gcccacagcg ccggcgctgg cggtttgagg gaggcggcga tggcaagcct tgccgacaat
     1141 ctcggcgctg ccccggcagg cgggcccgcg ccccacaggg ctgggcaggt cagcggctgg
     1201 cggctggcgc tgttttcctc ccagtcgatg gcgctggcag ctgtcgaggt gccgctcagc
     1261 acctatgtgc cgccgctcta cgcttcggct ttcggcttca gcctgaccac ggtggggctg
     1321 atcttcctgg ccgcgcggct gtgggatgcg gtgatcgatc cgggcatcgg catcctttca
     1381 gaccgcacgc gcagccgctg gggccgccgc cgcccgtgga tcgcgctggg cggcgcgatc
     1441 ttcgcggtgg gcgcggtacc ggtgttcctg ccgccggcgt ggtttgggcc ggcggcgctg
     1501 tcggccgcgc tgttcacgct ctatctgggc tattcgatga tcgccacgcc ctttgcggcg
     1561 tggagcggcg aattgtcggc cctttatcac gagcgcaccc gcgttgccac ctatgcgcag
     1621 gggatgacct cggtggcgct gctgctggcg ctggtgttgc ccagcctgct ggtgcgccag
     1681 ttcgaacatc agccgcgcct gcaactggcg gcaatggggg cgatggtgtt tgccctgctg
     1741 atcgccacgc tgccgctggg cctgcgcgcc ttgccggaac ccgcgcccga ggccggcccc
     1801 ctgcccccgc tgcggctggg cgaaagcctg aaggtggtgt tcaccgaaag gctgctgccg
     1861 cgagtgctgg cctcgaattt cgcggtgcgc ctgggccagg gcgtgcgcac ggcgctgttt
     1921 gtgttctttg tcacgcatta tctcgggcgg ccttcctggg cgccggggct gtttctgttg
     1981 caatatgtgt tcggcgtgtt tgcctgcccc atctggctgg cggtgggccg gcggctgggc
     2041 aaggcccgcg cggcggtggc cggcgaactg gtgcaggtgg cgatcaacct ggccctgctg
     2101 gggctggccc atgacagcat cgcgctgctg gtcggcctga cggtggcgca gggcctggcg
     2161 cagggctccg gcaatctgat gctgcgctcg attgtcgccg atgtcgccga caatcaccgc
     2221 ctgtggagcg gcgaggatcg catcggcctg ttctattcgg tattttccct gtcagacaaa
     2281 gccgcttttg ccgtcgccgt gggggtggcg ctgccgctgg cggcatggct gggtttcgac
     2341 cccaaacacg ccaattcggc agcggtgctg atgcacctca aatgggtttt cgccctgggc
     2401 ccggcgctgg cgcatctgtt ttccgccgcc ctcatccgcg gctttcccct gaacgaggaa
     2461 cgccacggcg aaatccgccg cgcattggcc gcgcgtgatg cggccggcct catttccgat
     2521 gccagccgcg aacgctggct ggccgaggcc gacgactgat cgccaaaggc gggggcaggg
     2581 atatcgccct gcccccgcat ggcccggctg atcagggcgt gacgatcggg aaggcgggat
     2641 cgggcttgtc gatccagccg ccaaagcggc gcagcgccag cagatcgccc gtaacgcttg
     2701 cctcgccggc catgatcttc gcggccagcg gttccccgcc ggtctgggcg gccagcagat
     2761 cggggtaatt cacgttcaac gtcacatcgg gctggccgat gatccccttg tggtgggtca
     2821 gcacgccatg ggcgatcacc accgtccagc tttcgccgcg atcgggaaag acaaaaccca
     2881 gccgcagcgc ctgatcgccc accttttccg catccagccg ggtggccagc acgtcgaaca
     2941 ggtcgtccgg cggcaggctg gcggcgatgc cggccttggc agcggtgttg gtcaccttca
     3001 cgccgcccag cagctcctgc gcgccggtga ggtagatgtt gcgccagacc gaattctcgc
     3061 tggcccaggc cagttgctga taggcccgcg ccagcagggc ccgcgcggcg cgatcggccg
     3121 gatcggcaaa caccgcgcgg ttgagcagtt cggccgccca ggcataatcg cccttgtcat
     3181 aggccgcctg cgccagtgca cgcacccgcg ccgcgccccc cagtgcctcg acatagcgct
     3241 ggccgccctc ggccggcggc aggggcgcca gatgcaccgg attggcatca taccagccca
     3301 gataatactg atagaccgcg cgcgcgttga agctgagcga tccataatag gggcggtcgt
     3361 accattcctt ctgcaaggcg gccggcaggc tgatcttcgc ggcgatctcg tcggcccgca
     3421 ggcccttgtt catccagcgc acggtctggt cgtgcaggaa ggcgtaggcg tcgcggtgct
     3481 tgcccagata gtcggtgatt tcgccggcgc cccagcgcgg ccagccgtgg ctggtgatca
     3541 gcacctcggc ccctgcgaag aaatccatcg cctcggtcag cccctgcgcc caggcatggg
     3601 cgttgcggat cacggcgccg cgcggggtga ggatattgtg ctgggtgaca ttggcgttct
     3661 cggccagatc ggccacctgc caatcgggaa agcccaggtt catttcggcc ggggcctcgg
     3721 tgccgggcgt cagctggaag cggatgtgca cgccatcgat ggtgatctcg gtgccggtct
     3781 ggctgatctc gcgggtgggg gcgatcagcc cctgcgagcc cagcgccgcc cccagcccga
     3841 tgcccgaacc cagcgatccg gtggggccgg cggccagcgg cacgccaaac tgatagctgg
     3901 cgcgcctttg catggccggg ccagcgatga tgttttccga aaccgccgcc gcgcgaaagc
     3961 cggccggggc gatgatcggc gcccccggcg ccagatcggc ggcaaaggcc cccgcgccgc
     4021 cgaaatgatc gacatggctg tgggtgtaga tgatgccggt gatcggccat ttgcccagct
     4081 gctggctgac cagctcatag gcggcgcgcg cggtttcggt cgtggtcagc gtgtcgatca
     4141 gcacccagcc cttttcgccg cgcacgaagg tgatgttggc cacatcgaag ccgcgcacct
     4201 gccagatgcg cgggctcacc ctgaacaggc cgtgcttggc cagaaggccg gtgtgatacc
     4261 acaggctggg gttgacggtg gccggcgccg ggcccttcac aaaggcatag gcatcaaggt
     4321 tccacaccac atggccatcg gcggcgcgga tcagcggatc ggcgcgggtg gccacaaagc
     4381 cgcggctggc aaaatcctcg tcgcgccgat cggcgaaggg ggcgctggcg gcggcggcgg
     4441 cctgcgcggc cagcgtccgg gcactgggtg gatcgaaagc gggtggatcg aaagcgggcg
     4501 gatcggcagg cggggtttgg gcgctggccg gcacgcaaag cgcggcggtg gccagcagca
     4561 ggcactggcg gaaaatacgc atggggc
//
LOCUS       DASRYE010000135        10010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_253277,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000135 DASRYE010000000
VERSION     DASRYE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253277"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFF98_07365"
     CDS             <1..256
                     /locus_tag="VFF98_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZU63484.1"
                     /translation="TFNFVLNNELTYSDKRLRGLVPVLRGWGKFALTCSFGLLTNVAT
                     AAALKRMHFHDVVAVLVGIVLGSVWNFALSSKFVWGKYGN"
     gene            291..527
                     /locus_tag="VFF98_07370"
     CDS             291..527
                     /locus_tag="VFF98_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63485.1"
                     /translation="MNLPAGQGGRLPHWLFAALVVAICLPFALVTVPPLLDMPGHMAA
                     SAIEAAPANSSLWRFYSWHWALIRTWAERCCCIC"
     gene            509..958
                     /locus_tag="VFF98_07375"
     CDS             509..958
                     /locus_tag="VFF98_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63486.1"
                     /translation="MLLHLLIPLMGPVRAGWLAAGLVLAARLLNPVGWQGIGLALCFV
                     YSQPWLYGFLNFTLASGLALLVFALWLGRAGHHWQQAALLLLAQPVLLLCHAMRWVGW
                     CWPRRWRAMCWAPASRMCGPAGPGPRRRRARWAKAGRCWARWRCWGC"
     gene            974..1537
                     /locus_tag="VFF98_07380"
     CDS             974..1537
                     /locus_tag="VFF98_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63487.1"
                     /translation="MPAAQAAGGGVSWMWVMKPWAALVVLQDQSQLFDLLGEGLCYGV
                     LAAGWLLGARWRWRQAGPALVLGLLFLVLPHRIAGSAGVDTRFLPLALGLALATQDWS
                     AARPAIARVVLAGGLLLLLARLAVLGAGFAAYARSYDANLAALAQVPLAGMDYLWLVD
                     TGAPAQPVPGLQPVWQQGRSSLYRLRR"
     gene            complement(1646..2980)
                     /locus_tag="VFF98_07385"
     CDS             complement(1646..2980)
                     /locus_tag="VFF98_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HZU63488.1"
                     /translation="MPSLHRRRLQCYLALVLADIAMLFISFAIAGQMADGVHGMAESL
                     FAAQVILPIFLTVALSNNAYSVSALQSTEHGMSRVLAALGAAIVVVMVLAFYARPVHG
                     LGRLPLTLDAVIAAIMLPTGRVLMRPLVRLRCGASAINQLVIDDGGPAIQLSGAFHVN
                     AESFGLNPSLNDPHALDRIGMVMRNADRVIVTTPPERRQLWAFILKSASVDGEVIDEA
                     VAELSARGARMLGNQGVLQVSVGPLGMRARISKRLFDIIAAGSALVLLAPLLLAVAAA
                     IKLEDGGPVLFVQRRVGRDNRFFRIFKFRSMRPTRAGRNGDVSASRGDNRITRIGRII
                     RATSIDELPQLLNVLRGDMSVVGPRPHAIGSLAGDKLFWEVDSRYWQRHALKPGLTGL
                     AQVRGWRGATDREEDLIGRINADLEYLRGWSLWRDVWILIATVRVVAHDKAF"
     gene            3377..4078
                     /locus_tag="VFF98_07390"
     CDS             3377..4078
                     /locus_tag="VFF98_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63489.1"
                     /translation="MRRIVLAVIVAAMAGSPALAMHRHHHAAQAKPAHGKAHHGKAHH
                     DKGHHGKAKPAHTKPAHTKAGHKAGAHGHHATARQRHAEAAAHQAADKPHGTRHHGHQ
                     GAGVARHHGAAAARHGKHAASEAGKPHTHKTHAGKAHAGKVHAGKAHAGKAQAHAHAH
                     KGHAHKAHTGKAAKAVGGRHHAAGAAHRHHAGHAASHRHGAAAPQYRTPHYEAPADSG
                     SGFNASFKDLRPNGQ"
     gene            4461..4811
                     /locus_tag="VFF98_07395"
     CDS             4461..4811
                     /locus_tag="VFF98_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63490.1"
                     /translation="MTSIFHKARIAAVFGLIALAVPGAASAQTAWQHHHPARAEINHR
                     VRHQEHQITRERREGKITAGQAHALRAQDRAIRKEERADARANHAHGHLTRGQAHALN
                     GQLNANHRAIEQNR"
     gene            5140..6423
                     /locus_tag="VFF98_07400"
     CDS             5140..6423
                     /locus_tag="VFF98_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HZU63491.1"
                     /translation="MAISTPVQPAGADAAGHVDAPELRLFVMALFFVFGGITSLNDVI
                     IPKLKELFTLDYTQAMLVQTAFFAAYAIIGLPGAALVKRLGYMRGATVGLLTMMAGCL
                     LFIPASQSARFGVFLFALFVLAAGVTIVQVVTNPLISLLGPARSAHSRLTFAQGFNSL
                     GTVIFPKVGAALILGGLAAVSARDLTGAALDAYRVAETRTVVHTYLGLAAALALVAGV
                     VWMNRNRLRGERHAEGSVFAGFSLLRRLRFGWGAASIFLYVGAEVATGSLIVSYMEQP
                     RVLGLSALAAGGLIPYYWGGALIGRFAGSAVLARFSPGKVLACNAAGAIALLALSANS
                     SGLLAAGSLLAVGLMNSIMFPTIFSLASEGLGARAADGSGVICVAIVGGAVMPPLAGA
                     LADATGNLALALVVPALCYAVIGAFGLYARRPAAG"
     gene            complement(6447..7574)
                     /locus_tag="VFF98_07405"
     CDS             complement(6447..7574)
                     /locus_tag="VFF98_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZU63492.1"
                     /translation="MRQIAALPYRRRSDAADSPTEVMLITSRGGNRWVLPKGNRMKGL
                     PPHAAAEQEAWEEAGVHGLICPTPLGDYSARKLLKTGEALPLNIRVYPLAVTEELTDW
                     PEASQRNRRWFPIAEAVEAVEEDQLRTLMRNFREADFAPPASNGPASASELWQMLLGL
                     RRAMQWRAMMPGQRGFFLMFEAHGQIVHSAAESLARLVQGGPDMATHIADIIEREDEA
                     DAITRDVLIDARRRWFPPFGKRATVRLISALDDVVDQMQGIANLIGIYEIASFAPEMR
                     DMAAIILDSARLVAEALPLLRQIHIHADRLHALTERLVRMENQADGIHMQGLKKAYLA
                     LGAEQPMAYFITRELYRQLELVVDGFEDIANEIDGLVIDAA"
     gene            complement(7693..8073)
                     /locus_tag="VFF98_07410"
     CDS             complement(7693..8073)
                     /locus_tag="VFF98_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63493.1"
                     /translation="MEHQAAQLKIKLQIYCGEEIAMGPGKADLLQAIAAHGSITAAGK
                     AMGMSYRRAWLLVDVMNRCWAEPLVATAPGRAGGSGARLTPAGKAVLATYLALVAAAG
                     TLRQGHDWQALSAQLRLAPLEKQG"
     gene            complement(8103..9620)
                     /locus_tag="VFF98_07415"
     CDS             complement(8103..9620)
                     /locus_tag="VFF98_07415"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin-coupled sensor protein"
                     /protein_id="HZU63494.1"
                     /translation="MLSNADARAKLAPLDETNYRAFPRIKAALSRHARKALDRLYGRI
                     AADPVTAALLPTDEGRTRAANAQFNHWQQLFAGRLDDAAIARSEKIGDVHARIGLTPA
                     YYVSGYALMLEELINAMMSEGALGHVPGQKQRGQTVATLVKTALLDMEIALSAYFTAD
                     ARARVEVVDQMGEALKAIAKGDLRASLTDIPKTYEQIAKDFHNMRYQVSSMVVQMKDA
                     SENVEVGAREISAAAHDLAHRTERQAATLARTAEVLREVADGIGTTARSAQEVNNSIA
                     AVDSQAKHGGEIVEAAVVAMDKIKHSSQEIASITDVIEAIAFQTNLLALNAGVEAARA
                     GDAGKGFAVVASEVRALAHRTTESAKNIKELIGKSAEDVNQGVDLVARTGSALEQIIQ
                     QVSQTTDQAHDIAHYAETQAEAMQQISAEIQQMDLNTQQNAAMVEESNAAAHGLKEQA
                     ATMARIVGQFQLERRIEPRDGGRSPSQGDVARRRDKAAQGRAATQALAGKVVNLW"
     gene            complement(9793..>10010)
                     /locus_tag="VFF98_07420"
     CDS             complement(9793..>10010)
                     /locus_tag="VFF98_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZU63495.1"
                     /translation="PRARDEMLARTHSVFAPWTIVRADHKPSARAAVIRDLLARCGEN
                     DEGLGQPDPTTCFLYDPIAHEKGWLAD"
BASE COUNT         1510 a         3296 c         3462 g         1742 t
ORIGIN      
        1 gaccttcaac ttcgtgctca acaacgagct gacctattcc gacaagcgcc tgcgcgggct
       61 ggtgccggtg ctgcgcggct ggggcaaatt tgcgctcacc tgctcgttcg gcctgctcac
      121 caatgtggcc accgccgctg ccctcaagcg catgcatttc cacgatgtcg tcgccgtgct
      181 cgtcggcatc gtcctcggct cggtctggaa cttcgccctc agctccaaat tcgtctgggg
      241 caaatacggc aattgaacgc aggcaaaggg gatctttgtt gggcaagctg atgaacctgc
      301 ctgccggcca gggcggcagg ctgccgcact ggctgtttgc cgccctggtg gtggccatct
      361 gcctgccttt cgcgctggtc acggtgccgc ctctgctcga catgccgggc catatggccg
      421 cctcggcgat cgaggcggcg cctgccaaca gctcgctctg gcgtttttac agctggcatt
      481 gggcgctcat ccgaacatgg gcggagaggt gctgctgcat ctgctgatcc cgctcatggg
      541 gccggtgcgg gcgggctggc tggccgcagg gctggtgttg gccgcgcggc tgttgaaccc
      601 tgtgggctgg cagggcattg gccttgccct gtgctttgtc tacagccagc cttggctcta
      661 cggtttcctc aatttcacgc tggcaagcgg gctggcgctg ctggtttttg ccctgtggct
      721 gggccgggcc gggcatcatt ggcagcaggc ggcgctgctg ctgctggcgc agccggtgct
      781 gctgctttgc catgccatgc gatgggtggg ctggtgctgg ccgcggcggt ggcgggccat
      841 gtgctgggcg cccgcatcga ggatgtgcgg gccggcgggg cctggcccac ggcggcggcg
      901 cgcgcgctgg gcgaaggctg gccgctgctg ggcacggtgg cgatgctggg gctgctgaag
      961 gggctggggc cgcatgccgg cggcccaggc cgcgggcggc ggcgtatcgt ggatgtgggt
     1021 gatgaagccc tgggcggcgc tggtggtgct gcaggatcag tcgcagctgt tcgatctgct
     1081 gggcgagggg ctgtgctatg gcgtgctggc ggccggctgg ctgctgggcg cccgctggcg
     1141 ttggcggcag gcggggccgg cgctggtgct ggggctgctg tttctcgtct tgccccaccg
     1201 gatcgccggc agcgctgggg tggatacccg ctttctgccg ctggcgctgg ggctggcgct
     1261 ggccacgcag gactggagcg cggcgcggcc cgccatcgcg cgggtggtgc tggccggcgg
     1321 gctgctgctg ctgctggcgc ggctggcggt gctgggggcg ggttttgccg cctatgctcg
     1381 cagctatgac gccaatctgg ccgcgctggc gcaggtgccg ctggccggca tggattacct
     1441 gtggctggtc gataccggcg cgcccgcgca gccggtgcct ggcctacaac cggtgtggca
     1501 acaggggcgc agcagcctct accgcctgcg ccgctaacaa aactgtaact aacgcagaac
     1561 aaacaacaag gccgcgcccc gaatcaagag gggcgcggcc ctcgcctgac ggccgcgcgc
     1621 cctgtggctg cgttatgggg cagggttaga aggccttgtc gtgggccacc acccgcaccg
     1681 tggcgatgag gatccagaca tcgcgccaca aggaccagcc acgcagatat tccagatcgg
     1741 cgttgatgcg cccgatcagg tcttcctcgc gatcggtggc gccgcgccag ccgcgcacct
     1801 gggcaaggcc ggtcaggccc ggcttcagcg catggcgctg ccagtagcgc gaatcgactt
     1861 cccagaacag cttgtcgccc gccagcgagc cgatggcatg cgggcgcggg cccaccacgc
     1921 tcatgtcgcc gcgcagcacg ttgagcagct ggggcagctc gtcgatgctg gtggcgcgga
     1981 tgatgcggcc gatgcgggtg atgcggttat ccccgcgcga ggccgagaca tcgccgttgc
     2041 ggcccgcgcg ggtggggcgc atgctgcgga acttgaagat gcggaagaag cggttgtcgc
     2101 ggcccacgcg ccgctgcacg aagagcaccg ggccgccatc ttccagcttg atcgccgcgg
     2161 ccacagccag caggagcggc gccagcagca ccagcgccga gcccgccgcg ataatgtcga
     2221 acaggcgctt ggaaatgcgc gcgcgcatgc ccagcggccc caccgatacc tgcagcacgc
     2281 cctgattgcc cagcatgcgt gcgccgcgcg ccgacagttc ggccaccgcc tcgtcgatca
     2341 cttcgccatc aacgctggcg ctcttgagga tgaaggccca cagctggcgc cgttcgggcg
     2401 gggtggtcac gatcacgcgg tcggcgttgc gcatcaccat gccgatgcga tcgagcgcat
     2461 ggggatcgtt cagcgagggg ttcaggccaa agctttcggc gttgacatga aaggcgccgg
     2521 acagctggat ggccgggccg ccatcgtcga tcaccagctg gttgatcgcg ctggccccgc
     2581 agcgcaggcg caccagcggg cgcatcagca cccggccggt gggcagcatg atcgccgcga
     2641 tcaccgcatc cagcgtgagc ggcaggcgcc ccagcccgtg cactggccgg gcatagaagg
     2701 ccagcaccat caccaccaca atggccgcgc ccagcgccgc cagcacgcgc gacatgccgt
     2761 gttcggtgct ttgcagcgca ctgactgaat acgcattatt acttagtgca acagtcagga
     2821 aaatgggcag aatcacttgt gcggcaaaca gggattccgc catgccgtgc acgccatcgg
     2881 ccatctggcc ggcaatggcg aagctgatga acagcatcgc gatgtcggcc agaacaagcg
     2941 ccagatagca ttgcagccgg cggcggtgca aagagggcat aagcggtgcg cgaatagcgc
     3001 gtgcgcgctg tttggcgcgt ttgcgcaagg agccgtcaag ccgagcggtc tgtgcgttca
     3061 tgccaagcta cctcgggtcc ttcaagtgtg gccgcggttg gagattcaac cgcggatacg
     3121 catatgtatc taggtatata cacttggaaa tgttgcagcg caatgtaaac gtcggcgtaa
     3181 ttcgacgaac agaatacgcg cgaatactag gttttcgttt gcatggcgga gtatgcaaac
     3241 tgcgccgggc ccacctgcgg ccagggtgaa gcgttttcgg cggggcttgc caggcaggcc
     3301 gcaggcgcat tagcagagcg ccaccaggcg cagcgggcga ttctggcctt tgtcgagtgt
     3361 gagaggaatg aagcctatgc gtcggatcgt gctggcggtg atcgtggcgg ccatggcagg
     3421 atcgccggcc ctggcaatgc atcgccatca tcatgcggcc caggccaagc cggcccatgg
     3481 caaggcccat catggcaagg cccatcatga taaggggcat cacggcaagg ccaagcctgc
     3541 ccataccaag cccgcccata ccaaggctgg ccataaggcc ggggcgcatg gccatcatgc
     3601 cacggcccgg cagcgccacg ccgaagcagc ggcgcatcag gcggcagaca agccgcatgg
     3661 caccaggcat cacggccatc agggcgcggg cgtggcgcgg caccatggcg ctgctgctgc
     3721 ccgccatggc aagcacgcgg ccagcgaggc cggcaagccg cacacgcata aaacgcatgc
     3781 cggcaaggcc cacgccggga aagtccacgc cggcaaggcc cacgctggca aagcccaggc
     3841 gcacgcccac gcccacaagg gccatgccca caaggcccat actggcaagg cggcaaaggc
     3901 ggttggcggg cggcatcatg cggccggcgc ggcgcatcgc caccatgccg gccatgcggc
     3961 cagccaccgc catggcgcgg cggccccgca ataccgcacc ccgcattacg aggcgccggc
     4021 cgatagcggc agcggcttca acgccagctt caaggatctg cgccccaacg gccagtgatc
     4081 tgcccggcgg gcgccggcgc agaagggatg cccggcgctt gctgacaggc gggccccggc
     4141 atggcgccag gcgctggcca agggcagggc gcctcagcgc cgggcatcag gaaaaaggaa
     4201 caggcttgcg gcatgctcct tcgggtcgcg gcgggatttt ggcagatccg ccaggggctg
     4261 gccatggcat tgcgtctcaa tcagcggccc atcgcttccg aaatggacag gagggcgcat
     4321 ccggcattca tataatttga tgcaattatg aacggtgtct gtggcggcct gtcctcaatc
     4381 gttcatcctg aagcgcctaa agctgtttgt gacgacggga gggagccccc catgccccgc
     4441 tcggatcaag gagcaaggcc atgaccagca tttttcacaa agcccgcatt gccgccgttt
     4501 tcggcctgat tgccctggcg gtgccgggcg cggcctcggc acaaaccgcc tggcagcacc
     4561 atcatccggc ccgcgccgag atcaaccacc gcgtgcgcca tcaggaacat cagatcaccc
     4621 gcgagcggcg cgaaggcaag atcaccgccg gccaggccca tgccctgcgc gcccaggatc
     4681 gcgccatccg caaggaagag cgcgccgatg cccgcgccaa ccacgcccat ggccacctca
     4741 cccgcggcca ggcccatgcg ctgaacggcc agctgaacgc caaccaccgc gccatcgagc
     4801 agaaccgctg atccagcggg gggcggccgg gcgaggggat cagggcaaac ccctgcacct
     4861 tcgtccggcc gtattcagca tagtgcaatg agaatcggct aaagcagccc tgtcgccccg
     4921 aatcgagagc catggaaccg gctgttgccc tctccatccc tctcaactgg ggcggcgccc
     4981 cttgcctgcc cgccgcgcct tcttttgccg cgccggtgct gtcgcgcctc ttccatacgt
     5041 ttgcgcagaa cttgccttgg cggccatcgc cgggcatccg cgcagccaag cgtttagcgg
     5101 caggccaaag caggccgccg gcaaagggag agtgcagcca tggccatttc cacccccgtt
     5161 cagccggcag gcgccgatgc ggccggccat gtggatgcgc ccgagctgcg ccttttcgtg
     5221 atggcgctgt tcttcgtctt tggcggcatc accagcctca acgatgtcat catccccaag
     5281 ctgaaagagc tgttcacgct ggattacacc caggcgatgc tggtgcagac ggccttcttc
     5341 gccgcctatg ccatcattgg cctgccgggc gcggcgctgg tgaagcggct gggctatatg
     5401 cgcggcgcca ccgtgggcct gctgacgatg atggccggct gcctgctgtt catccccgcc
     5461 tcgcagagcg cgcgctttgg cgtgttcctg tttgcgctgt tcgtgctggc ggcgggcgtc
     5521 accattgtgc aggtggtcac caatccgctg atttcgctgc tgggcccggc gcgttcggcc
     5581 cacagccggc tgacctttgc gcaagggttc aacagcctgg gcacggtgat cttccccaag
     5641 gtgggcgcag cgctgatcct ggggggcctg gcggcggttt cggcgaggga cctgaccggc
     5701 gccgcgctcg atgcctatcg cgtggctgaa acgcgcacgg tggtgcacac ctatctgggg
     5761 ctggcggcgg cgctggcgct ggtggccggc gtggtgtgga tgaaccgcaa ccgcctgcgg
     5821 ggcgaacgcc atgccgaggg cagcgtgttt gccggtttca gcctgctgcg ccgcttgcgc
     5881 tttggctggg gggcggcctc gatcttcctc tatgtgggcg cagaagtcgc caccggctcg
     5941 ctcatcgtca gctatatgga gcagccgcgc gtgctgggcc tgtcggcgct ggccgctggc
     6001 gggcttatcc cctattactg gggcggcgcg ctgatcgggc ggtttgccgg ctcggcggtg
     6061 ctggcgcgct tcagcccggg caaggtgctg gcgtgcaatg cggcgggcgc gattgccctg
     6121 ctggcgcttt cggccaacag cagcgggctg ctggcggcgg gcagcctcct ggcagtgggg
     6181 ctgatgaatt cgatcatgtt ccccaccatc ttctcgctgg ccagcgaagg gctgggtgcg
     6241 cgcgcggccg atggttcggg ggtgatttgc gtggcgatcg tgggcggggc ggtgatgccg
     6301 ccgctggccg gcgcgctggc cgatgccacc ggcaatctgg ccctggcgct ggtggtgccg
     6361 gcgctgtgct atgcggtgat cggcgccttt ggcctctatg cccggcgccc ggcggcgggc
     6421 tgaggctccg gcttaggccc aattgctcaa gccgcgtcga tcaccagccc gtcgatctcg
     6481 ttggcgatat cctcgaagcc atcgaccacc agttccagct ggcgatacag ctcgcgggtg
     6541 atgaaatagg ccatcggctg ctctgccccc agggccagat aggccttctt cagcccctgc
     6601 atgtggatgc catcggcctg gttttccatg cgcaccagcc gctcggtcag cgcatgcagc
     6661 cggtcggcgt ggatgtggat ctggcgcagc agcggcaggg cttcggccac cagccgggct
     6721 gaatcgagga tgatcgcggc catgtcgcgc atttcgggcg cgaagctggc gatctcgtag
     6781 atgccgatga ggttggcgat gccctgcatc tggtccacca catcgtcgag cgcgctgatc
     6841 agccgcaccg tggcgcgctt gccaaacggc ggaaaccagc gccggcgcgc atcgatcagc
     6901 acatcgcggg tgatcgcatc ggcctcatcc tcgcgctcga tgatgtcggc gatgtgggtg
     6961 gccatgtcgg gcccgccctg caccagccgc gccagcgatt cggcggcgga atggacgatc
     7021 tgcccatgcg cctcgaacat caggaagaag ccgcgctggc cgggcatcat cgcgcgccac
     7081 tgcatcgccc gcctcaggcc cagcagcatc tgccacagct cgctggcaga ggctgggccg
     7141 ttgctggcgg gcggcgcgaa atcggcctcg cggaagttgc gcatcagcgt gcgcagctgg
     7201 tcctcttcca ccgcttccac cgcctcggca atgggaaacc agcggcggtt gcgctggctg
     7261 gcctcgggcc agtcggtcag ctcctcggtc accgccagcg ggtagacgcg gatgttgagc
     7321 ggcagcgcct cgcccgtttt caacagcttg cgcgcgctgt aatcgcccag cggggtgggg
     7381 cagatcagcc cgtgcacgcc cgcttcctcc catgcctcct gttcggccgc ggcatggggc
     7441 ggcaggccct tcatgcggtt gcccttgggc agaacccagc ggttccctcc gcgcgaggtg
     7501 atcagcatca cttcggtggg gctgtcggcg gcatcactgc ggcgacggta gggcagggcg
     7561 gcaatctggc ggatggcggg aaatcctcat cggcaaggga acaggaaagc ggatacatgg
     7621 ccctgttggc gccaaggtca atcggcaatc gcaaataaac gccggtttct ggccggccgg
     7681 ctgccgctgc gctcagccct gcttctccag cggggcgagg cgcagctggg cagacagcgc
     7741 ctgccaatca tgcccctggc gcagcgtgcc ggcggccgcc accagcgcca gataggttgc
     7801 cagcaccgcc ttgcccgccg gggtcagccg ggcgccgctg ccgccggcgc ggcccggggc
     7861 ggtggccacc agcggctctg cccagcagcg gttcatcaca tccaccagca gccaggcccg
     7921 gcgatagctc atgcccatcg ccttgcccgc cgcggtgatc gagccatggg cggcgatcgc
     7981 ctgcagcaga tcggccttgc ccggccccat cgcaatttcc tcgccgcaat agatctgcag
     8041 cttgattttc agttgtgcgg cttggtgctc cattgggcct gattaggcga ttgttgcgca
     8101 tctcaccaga gattgacgac cttgccggcc agcgcctggg tggcggcgcg gccttgcgcg
     8161 gccttgtcgc gccggcgggc cacatcgccc tgcgaggggc tgcggccgcc atcgcgcggt
     8221 tctatgcggc gttccagctg gaactggccg acgatgcggg ccatggtggc ggcctgttcc
     8281 ttcagcccgt gggcggccgc gttcgattcc tccaccatgg cggcgttctg ctgggtgttg
     8341 aggtccatct gctggatctc ggcgctgatc tgctgcatcg cctcggcctg ggtttcggca
     8401 taatgggcga tatcgtgggc ctgatcagtg gtttgcgaca cctgctggat gatttgttcc
     8461 agcgcgctgc cggtgcgcgc caccagatcc acgccctggt tcacgtcttc cgccgatttg
     8521 ccgatcagct ccttgatgtt cttggccgat tcggtggtgc gatgggccag cgcgcgcact
     8581 tcgctggcca ccaccgcaaa gcccttgccg gcatcgccgg cgcgcgccgc ttccacgccg
     8641 gcgttcagcg ccagcaggtt ggtctggaag gcgatggcct cgatgacatc ggtgatcgag
     8701 gcgatttcct ggctggaatg cttgatcttg tccatggcaa ccacggccgc ctcgacgatc
     8761 tcgccgccat gcttggcctg gctgtctacc gcggcgatcg agttgttcac ttcctgggca
     8821 gagcgggcgg tagtgccgat gccatcggcc acttcgcgca gcacttcggc ggtgcgggca
     8881 agggtggcgg cctgccgctc ggtgcggtgg gccaggtcat gcgcggcggc ggaaatctcg
     8941 cgcgcgccca cttccacatt ttccgatgcg tctttcatct gcaccaccat gctgctcacc
     9001 tggtaacgca tgttgtggaa atccttggcg atctgctcgt aggtcttggg gatgtcggtc
     9061 agcgaggcgc gcaggtcgcc cttggcgatg gccttcagcg cctcgcccat ctggtccacc
     9121 acttccacgc gggccctggc atcggcggtg aaataggccg acagggcgat ttccatgtcg
     9181 agcagggcgg ttttcaccag cgtggccacg gtttgcccgc gctgcttctg gccgggcaca
     9241 tggcccagcg cgccctcgct catcatcgcg ttgatcagct cttccagcat cagcgcatag
     9301 ccgctgacgt aataggccgg cgtcagcccg atgcgggcgt gcacatcgcc gatcttttcc
     9361 gaacgggcga tggcggcatc gtcgagccgg ccggcgaaca attgctgcca gtggttgaac
     9421 tgggcattgg cggcgcgggt gcggccttcg tcggtgggca gcagcgcggc ggtaacggga
     9481 tctgcggcaa tgcgcccgta gagcctgtcg agcgccttgc gggcgtggcg ggaaagcgcg
     9541 gccttgatgc ggggaaaggc acggtagttc gtctcgtcga gcggcgccag cttggcgcgg
     9601 gcatcagcgt tggaaagcat ggccttgggg atccggtggc aagaggacaa gcgcggttgc
     9661 gcccacaccc cgccaccggc ccaaaccgcc acagggcgcc gggaggaagc aggacatggg
     9721 caacgctagc ccatgcttgg ttgacggctg ttatgcccgg gccccggtag tttgccggaa
     9781 gcactgtgct tctcaatcgg ccagccagcc cttttcatgg gcgatggggt cgtaaaggaa
     9841 gcaggtcgtc gggtcgggct ggcccagccc ctcgtcattc tcgccgcagc gggccagcag
     9901 gtcgcggatc acggctgcgc gcgcgcttgg cttgtggtct gcccgcacga tcgtccaggg
     9961 ggcgaacacg ctgtgggtgc gcgccagcat ctcgtcgcgc gcgcgggggt
//
LOCUS       DASRYE010000136         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_148704,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000136 DASRYE010000000
VERSION     DASRYE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148704"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFF98_07425"
     CDS             complement(<1..893)
                     /locus_tag="VFF98_07425"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HZU63496.1"
                     /translation="MARKIRVLVVDDSASVRQMMTAILSADPQIEVIAAASDPFVAAR
                     AIKEELPDVVTLDVEMPRMDGITFLRKLMSQCPVPVVMCSSLVEDGSDTLLQALECGA
                     VDIILKPRAGVADFLAEQAQRICDTVKAAAQARVQQRSAHQPLRPQQKLTADAMLPPP
                     GSRAMSRTTEMVVCIGASTGGTEALREVLEALPANAPGIVIVQHMPEHFTRTFAQRLD
                     ALCEVSVKEAEDGDTVMRGHVLIAPGGRHTLLARQGARYLVSVRDGPLVSRHRPSVDV
                     LFRSAAQSAGANAVGVILTGMG"
     gene            complement(900..1754)
                     /locus_tag="VFF98_07430"
     CDS             complement(900..1754)
                     /locus_tag="VFF98_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HZU63497.1"
                     /translation="MMPEPAPLLAPPAISPVRFRRIAQLIHSRTGIRMPDSKIHLIEG
                     RLMRKAREVGMANIDAYCDRVLNARPDDPLLTEFFNAVTTNKTDFFREPVHFDYLTTQ
                     LLPARHAAGQATLRCWSAAASSGMEAYTLAMVLAEAVQAGLLRDFEILATDLDTRVLA
                     EARRGVYRHADLAAVPATLRAAWFANARDRRRREARVIPALRSKVAFAQLNLMEAQYG
                     LGDPLDVIFCRNVLIYFDKDTQFRVISRLCEVLRPGGHLFLGHSETIHGLDLPLQAVG
                     STVFRKKG"
     gene            complement(1773..2312)
                     /locus_tag="VFF98_07435"
     CDS             complement(1773..2312)
                     /locus_tag="VFF98_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZU63498.1"
                     /translation="MSGERRAAAGGSPAADAPVQAVTFGIGSEIFAVPVAIVREILDH
                     QPPFRLPNAPDYFAGLIDVRGVAVPTIDLRRRLGLPAQAPTALTRIFILEVPAPDGPP
                     LLLGLTVDRALVVSAFAADQVEPPPRIGVRWRSEYITGVIRQAGSFIVLIDIARVLTS
                     EDAALIAEAATDCGSSAAA"
     gene            complement(2309..>2862)
                     /locus_tag="VFF98_07440"
     CDS             complement(2309..>2862)
                     /locus_tag="VFF98_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU63499.1"
                     /translation="SQEAATEISALSADTVKAATEAGEMLGRLVPDIRRTAELVAEIS
                     AACREQDIGSAQINTAIQQLDKVTQQNAGASEEISATAVELAAQSEELQGSVSFFRTH
                     AHAAPRPLPASTPARQPASAQRTQRAPLARAAKPARPIAAARTAAPRPDSIKDQQARV
                     KGFALDLTQGGPDDEDHDFGLVA"
BASE COUNT          428 a          979 c         1027 g          428 t
ORIGIN      
        1 cccatgccgg tcaggatcac gcccacggca ttggcgccgg ccgattgcgc ggccgagcgg
       61 aacagcacat ccaccgaggg ccggtggcgc gacaccagcg gcccatcgcg cacgctgacg
      121 agatagcgcg cgccctgccg cgccagcagc gtgtggcgcc cgcccggcgc gatcagcaca
      181 tggccgcgca tcaccgtgtc gccatcctcg gcctccttca cgctcacctc gcacagggca
      241 tccagccgct gggcaaaggt gcgggtgaaa tgctcgggca tgtgctgcac gatgacgatg
      301 cccggcgcat tggccggcag cgcttccagc acctcgcgca gcgcctcggt gccgccggtg
      361 gaagcgccga tgcacaccac catctcggtc gtgcggctca tcgcgcgcga tccgggcggg
      421 ggcagcatgg catcggcggt cagcttctgc tgcgggcgca ggggctggtg ggcgctgcgc
      481 tgctgcacgc gggcctgggc ggcggccttc accgtgtcgc agatgcgctg ggcctgttcg
      541 gccaggaaat cggcaacgcc ggcgcgcggc ttcaggatga tgtccaccgc gccgcattcc
      601 agcgcctgta gcagcgtgtc cgagccatcc tccaccagcg acgagcacat caccaccggc
      661 accgggcact ggctcatcag cttgcgcagg aaggtgatgc catccatccg cggcatctcg
      721 acatccagcg tcaccacatc aggcaattcc tccttgatgg cgcgggcggc cacgaaggga
      781 tcgctggccg cggcgatcac ctcgatctgc ggatcggccg agaggatcgc cgtcatcatc
      841 tggcggacgc tggcggaatc atcgaccacc agcacgcgga tcttgcgcgc catcgcggat
      901 caccctttct ttctgaacac ggtgctgccc accgcctgca gcggcagatc aagcccgtgg
      961 atcgtttcgg aatggcccag aaacaggtgg ccgcccggcc gcagcacctc gcacaaccgc
     1021 gagatgaccc tgaactgcgt gtccttgtcg aaatagatca gcacattgcg gcagaagatc
     1081 acatccagcg gatcgcccag gccatattgc gcttccatca ggttcagctg ggcaaaagcc
     1141 accttgctgc gcagcgccgg gatcacccgc gcctcgcgcc ggcgccggtc gcgggcattg
     1201 gcgaaccagg cggcgcgcaa ggtggccggc accgcggcca gatcggcatg gcgatagacc
     1261 ccgcgccgcg cctcggccag cacccgcgtg tcaagatcgg tggccaggat ctcgaaatcc
     1321 cgcagcaggc cggcctgcac ggcctctgcc agcaccatgg ccagcgtgta ggcttccatg
     1381 ccgctggagg cggccgcgct ccagcagcgc agggtggcct ggccggcggc atgccgggcc
     1441 ggcagcagct gcgtggtgag atagtcgaaa tgcaccggct cgcggaagaa gtcggtcttg
     1501 ttggtggtca ccgcgttgaa gaattcggtg agaaggggat cgtcgggccg ggcgttcagc
     1561 acccggtcgc aataggcatc gatgttggcc atgccgacct cgcgggcctt gcgcatcagc
     1621 cggccctcga tgaggtggat cttgctgtcg ggcatgcgga tgccggtgcg gctgtggatc
     1681 agctgggcaa tccggcgaaa gcgcacgggg ctgatcgccg gcggggcaag gaggggcgcg
     1741 ggttcgggca tcatgggcgg ggcagcgctt tctcaggcgg cggccgaaga tccgcagtcg
     1801 gtggcggcct cggcgatcag ggcggcgtcc tcgctggtca gcacccgggc gatgtcgatc
     1861 agcacgatga aactgcccgc ctggcggatc acgccggtga tatattcgct gcgccagcgc
     1921 acgccgatgc gcggcggcgg ctccacctga tcggcggcaa aggcgctgac caccagcgcg
     1981 cggtccaccg tgaggcccag cagcagcggc ggcccatcgg gggcgggcac ttcgaggatg
     2041 aagatgcggg tgagggcggt gggcgcctgc gcgggcaggc ccaggcgccg gcgcagatcg
     2101 atggtgggca cggcaacgcc gcgcacatcg atcaggccgg cgaaatagtc cggcgcgttg
     2161 ggcaggcgaa agggcggctg gtgatccagg atctcgcgca cgatggccac cggcacggcg
     2221 aagatctcgc tgccgatgcc aaaggtcacg gcctgcaccg gcgcgtcggc ggccgggctg
     2281 ccgcccgccg ccgcccttcg ctcgccgctc atgcgaccag cccgaaatcg tggtcctcgt
     2341 cgtccgggcc gccctgggtc aggtccagcg cgaagccctt cacgcgggcc tgctggtcct
     2401 tgatggaatc gggccggggg gctgcggtgc gggccgcggc gatgggcctt gccggctttg
     2461 ccgcccgggc cagcggcgcg cgctgcgtgc gctgtgcgct ggccggctgg cgggcgggcg
     2521 tgctggcggg cagggggcgc ggggcggcat gcgcatgggt gcggaagaag gacacgctgc
     2581 cctgcaattc ttccgattgc gcggccagct ccaccgccgt tgccgagatc tcctccgagg
     2641 cgccggcgtt ctgctgggtc accttgtcga gctgctggat ggcggtgttg atctgcgccg
     2701 aaccgatatc ctgctcgcgg caggcggcgc tgatctcggc caccagctcg gcggtgcggc
     2761 ggatatcggg caccaggcgg cccagcatct cgcccgcctc ggtcgccgcc ttcacggtat
     2821 cggccgacag cgcgctgatc tcggtggcgg cttcctggct gc
//
LOCUS       DASRYE010000137        19114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_218677,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000137 DASRYE010000000
VERSION     DASRYE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19114
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218677"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFF98_07445"
     CDS             <1..201
                     /locus_tag="VFF98_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63500.1"
                     /translation="AHSPAPASSRLAGRQARKSRARAGKVVVMAGSWAPRLSGWDENT
                     LLTASFNHVQCMSAQKVSAMVR"
     gene            complement(314..1444)
                     /locus_tag="VFF98_07450"
     CDS             complement(314..1444)
                     /locus_tag="VFF98_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63501.1"
                     /translation="MEPLPRLVSTAHACSTAHACGTVKRPWRDRDFHFELAPVDPRGG
                     QIRTDALIRIDNYGEAAELALSGMALRMSDGRPEGIKWVKPAALRIEDEPVAVVDALW
                     QHTLLPPPVAPAALADDIARFLGQYTQMLELLVSAPARQAIDPLAPCHLTRLAQTAHD
                     SAFRTGADLAISDTRRREIARVLRLMLGLMPAPAPHRPADSLPALWHTLLAAHLRAAL
                     VAGEVEALDLGGTIAENLALLAGMSHQGARNALARDGISTLRARLEAPALLGWLSQRC
                     GFAPLRCGERPAARASARALDLLRRMPVLAALDSLRAETAGDAALLAERQLRAQLVRH
                     GRAPAGLWRRYARAAGLCIDSFLHGLGTSAIDALPVPAARQG"
     gene            complement(1454..3103)
                     /locus_tag="VFF98_07455"
     CDS             complement(1454..3103)
                     /locus_tag="VFF98_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63502.1"
                     /translation="MKYASTLLAACAAGSLLAPIAPAQAATKTVHHRAAHKRAAPPRA
                     ENSGVNQELAELRAEVNALHQELASQRQSAATTQTQVAQTEAQMNALHDEVAQAPKAA
                     HTAAREEVAVALDKEHKKGYADFKGLHITPGGFLEMAGIYRQRFQGNDMATSFAIPFP
                     NNRAGTASESRLSARQSRLSFLAQGKVNKDVTLSMYGEFDFLGGAQTANSNESNSFNP
                     RIRHLYGTIDWAHNGGGIHLLAGQNWSLITLNTKGITPRNELTPPQIDAQYIPGFFWA
                     RQPQLRLTVDTADHKLWLAVSAENAATTTVGTVPTSLITYNATAGSGFDTANSLSLNG
                     TPDFVGKVAYEGSVAGRSLHVEGFALSRTFTAHLNGGYNINRAGYGFGGGAVFQAVPG
                     KLDLQFSGIVGKGIGRYGSAQLGDVTFDANGMIHPVGEFMLLGGATFHATKRLDLYLF
                     AGKEQETGYSFASGYGNASSTANNAGCFIEGGTCSGNTRRVQQLTTGFWNKLYSGSFG
                     HAQVGLQYAYTQRQLFADANGIMPQVGENIGFLSFRYYPFN"
     gene            3391..3891
                     /locus_tag="VFF98_07460"
     CDS             3391..3891
                     /locus_tag="VFF98_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63503.1"
                     /translation="MTNSFNRSVNRSIRTLALGGLLALGAGGVAQAHDAPYQAHLYGR
                     APSAGLHFADLEYAMPSDPLTWSSDPGWEAHHTPARFMTDAQSALAAAVPAGLSAAEA
                     AQRLHMAGAHCRQPAGAELSCTYKTVETPRTYWDAVTWTVAMPLTADGRVASMSVTRD
                     WTRRYL"
     gene            3929..4618
                     /locus_tag="VFF98_07465"
     CDS             3929..4618
                     /locus_tag="VFF98_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU63504.1"
                     /translation="MTNMARILVVEDDPAMAGRIQTRLEAEGYETCHAASGGEGLVHV
                     QDGRFDAITLDRMLPDMDGLELLGRLRAQSVATPVLLISSLGDVDERIAGLRAGGDDY
                     ITKPFSADEMAMRVEVLLRRGQPSAAEPATYVNGDLRMDFIARKVWLAGEPVQLLQKE
                     FLLLQFFVRHPGQTITRRMIFEQVWGYRFDPSDNLINVHVSKLRRKLERPDHPSPIAT
                     VKGEGYRFDPV"
     gene            4622..5983
                     /locus_tag="VFF98_07470"
     CDS             4622..5983
                     /locus_tag="VFF98_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU63505.1"
                     /translation="MLPLLRMRWSDIRRSATFRLTTLLGCVFLVAVVALLSLTYVLTE
                     KELVARNDQVVYKEARRLAAVPRGRMTAAVDAAIANNASGLNYFGLIGSNGQVLAGNM
                     PQVPQLLPGEPFEIPAGAGVPVPLRVYVLPLADGCRLVVARDNTTLADLRDRVLAITV
                     GSGLLVAVSAAVVGFWLSLGMLKRIGRMTDTAQHIARGELGQRMPVAGRHDELDLIAT
                     TVNAMMEEIERLLQQVKGATDAIAHDLRTPLAHLRHRLQGLSEREETASPIVERAVEE
                     LDLVLSRFTALLRISELEVAGRRAGFGLLDPMAVVAEVCELYEPLAEERGIRLTLSGD
                     FGQVVEGDRLLLIEAIGNLVENAIKFIARGGTVQLQVAATGERVAIEVRDNGPGIAEA
                     ERALVLGRFHRGAGGRHAPGSGLGLSLVAAIAHLHGFALSLGDAAPGLVVRIIAPRAG
                     EGA"
     gene            6117..9311
                     /locus_tag="VFF98_07475"
     CDS             6117..9311
                     /locus_tag="VFF98_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63506.1"
                     /translation="MLEIVKIALGKPYTFVVMAILIVLGGSIAWMETPTDIFPDIRMP
                     VIAAVWTYNGLQPSDMSGRVVYFYERQLTSTVNDIDHIESQSLNGVGVVKIFFHTGVD
                     IRTATAQVTAASQTVLKQMPPGITPPLILNYNASTVPILQLALSSPSLSEQQIFDLGQ
                     NSIRASLATVQGAAIPSPYGGKVRQIQVDLDPVALAARHLTPADVGTALAAQNQIIPA
                     GDARIGGYEYAVRLNNSPEAIAELNNLPIRTLNGTTVYMRDVALVHDGAPPQTNVVRV
                     DGRRAVLMTVLKAGSASTIAIVDGVKALLPGLAQTLPSDLHIAALADQSLFVKGAISG
                     VVREGAIAAALTSLMILLFLGSWRSTVIIATSIPLAVLGAVAALAASGETLNIMTLGG
                     LSLAVGILVDDATVTIENINWHLEQGKGVQQAILDGAAQIVQPALVSLLCICIAFVPM
                     FFLGGVAGFLFAPMAKAVVFAMMASFVLSRTLVPTMGNYLLRAHASAHTADVMASHDA
                     SAGRPATRNPLRRFQHGFETRFEVARRWYVDLLGLALGHGRAVVLGMAGVVAVSLALV
                     PFLGRDFFPAVDSGQISLHVRAPVGTRIEDSAALFDRVEAQIRSQLPAGEIASVVDNI
                     GMPVSGINLAYSNTGGIGPQDGDILVTLKEGHAPTAQYVRQLRARLPGAFPGVSFSFL
                     PADIVSQILNFGAPAPIDVMITGPDGKADEAYANRLLADLQHVPGLADAHVQQASNYP
                     GLAVDVDRSQADRLGITESDVTRSLSVDLAGSLQTAPAFWLNPKNGVSYPIVVQAPQY
                     RAGTLSDLANVPVAASGAAGLQTLSAIGSTRLERTPAVIAHYSLQQALDIYATTDGRD
                     LGAVAGDLQKVIDAHKADLPKGAHVDLRGQVQTMNAAFSGLLLGLLGAIALIYLLIVV
                     NFQSWADPAAIISALPAALAGICWMLFACHTTLSVPALTGAIMCMGVATANSVLVVSF
                     ARERLAACGNALEAALEAGSTRFRPVLMTALAMIIGMGPMALGLGEGGEQNAPLGRAV
                     IGGLLFATMATLVFVPVVFSLIHRNSSLGETTHD"
     gene            9304..10470
                     /locus_tag="VFF98_07480"
     CDS             9304..10470
                     /locus_tag="VFF98_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63507.1"
                     /translation="MTDFSPSPAAAPRGLRKAGIAIGAVALVVVLAGSAVRFHAAADA
                     RNVAASQGTPAVHLLQPQPLGASQQLVEPGAIAAWTDARLFARVPGYVHGWYADIGQR
                     VAAGAPLGRIDTPELDEQINEARAALVRARAEAALARTTSARWQDLLVSHSVSRQEAD
                     EKAADAATHAAGVAEAEAHLGRLTAMKAFATLRAPFAGVITLRNADVGDLVGPGATGQ
                     QPMFALADDHRLRIYVNVPQQFTPLMHAGLTARLTVPAWPGRSFAARVLDQSGAIDGQ
                     TGALKVELVADNPDGALRPGGFVQVGFDLPMPAGHLSVPVSALIQRGGGTRVATVDGA
                     GHAHLLPVTIGRDFGSTVEVVAGLGAGTPVVDSPPDSLAEGEAVDVVAKGGAHG"
     gene            10463..11899
                     /locus_tag="VFF98_07485"
     CDS             10463..11899
                     /locus_tag="VFF98_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU63508.1"
                     /translation="MAERRLPRWLATGVLATSMLAGCNMAPAYRPPQLAMPAHFRESA
                     GPWAPASPGRARIGAQWWQALGSAELDGLEARLLASSPDLAAALARHDIARAALTEAR
                     ASALPQVDAGADITNNRQSANRPLRGSGQPDEYAADTVGAGASYELDLWGRVRNTVAA
                     AHANADASADDLAAARLSLTATLAQTYIALVGTDRDCAILADAVAVYGRAADVTRHRF
                     EEGIASGIDLGRADAQLASAQAALAEAQGQRARLEHAIATMVGTPASSFAIAPQAARL
                     AVLPVPAALPSALLQDRPDVAAAERRMYAANRGIGVARAALYPQIDLIGGGGVQDTAL
                     AGLMAAPNLFWSVGPQISLPLFDGGKRRARVRQAQGEWRAASALYRGAVLGAIGEVED
                     GLAMSRQLTEASAAQERAAAASGSAASLAYGLYVKGAASLLDVVTAQTTALTAARLAN
                     QTYTRRLQTGVDLVRALGGGVMAPATKA"
     gene            complement(11934..13358)
                     /locus_tag="VFF98_07490"
     CDS             complement(11934..13358)
                     /locus_tag="VFF98_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HZU63509.1"
                     /translation="MSLILADTANAAPEPLATTGAPEHPEAAKEDGANAVDAVPPAGL
                     LLPFAIQHVLVMYAGAVAVPLVVGRAMHLSIEHLAVLISADLVAAGLATLIQTLGLPG
                     IGIRLPIMMGVTFASVSPLLALIEAAGPAPGAAMGLLPTIYGAVIVSGIFGFLIAPFV
                     GRLTRFFPPTVTGTVILIIGLSLMRIAIDWAAGGQPSQPGYGAPLHLGIAFATLLIIL
                     GFMKYGRGLVRNGAVLLGVIAGAVLATALGETDFAPVASAPWFALVTPFQFGMPRFDM
                     PISLAMCFVMIVTMIESFGMFLAAGTLVGRPATTTDLARGLRADALGTVIGGVFNTFP
                     YTSYAQNIGLLSITGVRSRYVCAVGGVVLMMLGLCPKLAALVAALPVYVLGGAGFMMF
                     GMIAATGIRILSSVELTQGRIATIAVSVAMGLIPVLSDKFFQAMPAALTPLLHSGIVL
                     SSTTAILASLFFESQAEEAGEIHI"
     gene            complement(13437..15737)
                     /locus_tag="VFF98_07495"
     CDS             complement(13437..15737)
                     /locus_tag="VFF98_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63510.1"
                     /translation="MTYHRACAQALSVSALALTIALGSPARAEEAADAAAPQLEMITV
                     TAQKTNTGLQKTPIAISTLSGDQLARADIRSTVDLDKQVPGMTVSDAGAFPLNITIRG
                     VGYDGVQNLSAQPGVAFVENGVYIASPVALTGAFVDLGQLEVLRGPQGTVNGQNADGG
                     AINVTTNLPKLGVFAGSGEASYGSYNYNREKAMLNIPLTENLAIRATGQHEAHDGWYY
                     APNYPGTNHIGSENAWSGRVSALWKPTDKLTVTAWGELYSNDINGVGAKNIVDSISGV
                     RATSNDYADPEQTRSRIAAATVAYDLGFATVKSTTSYQYVYYNARTSPDMISTEQAWS
                     YYGVKDEIPVHSWSTNSVTQEVNLASKPGGKVDWIVGAFYLHSSGSEYVFETQQTAAQ
                     QSIPYSISYAAAYNASLYAAQSYLSGAYGLAFVSVSLAQRHSWAGYGQATWHITDKLR
                     LTGGLRYSIDSYHANTAANGTVATLVSEFSKMTGKAVVEYDVARHSTLYASFSTGVKP
                     GGANLNPASEVVPSAFKHEFVRAYEIGSKNEFFDRKLRLNLSAFYNDYRNLQADSEDP
                     LPYEGGMTNIPKSHTYGVEAEVSLILPEGWRIDGNAAGMGSKVDSHVHMLDAYVAQAI
                     NRANGGTYAGNDIADRTAAYYASDVYGHQLGRVPNFSSSLTISKSSEVADGKLDAALQ
                     TTYRSPFWQRVYNSPWDRVGSQFLMNLNLHYQPAHHPWYLELQVTNLTDSQDIATRYA
                     ENFGVGGVFDVAVPPRQFIGRVGVNF"
     gene            16044..17381
                     /locus_tag="VFF98_07500"
     CDS             16044..17381
                     /locus_tag="VFF98_07500"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZU63511.1"
                     /translation="MRIWISNPQAIFGEACAGGLVVADGRIAELVPAGARPTGPVDEV
                     MDASGHVVLPGLINTHHHFYQTLTRAHPAAINQALFGWLQALYPIWRRLSPEALELAS
                     EAAMAELLLSGVTTAADHHYVFPAGLEAAIDIQVEVARRLGLRVTLTRGSMSLSTADG
                     GLPPPEVVQDHDTILADSERLITRYHDAGDGAMVQIALAPCSPFSVTPQLMADSAALA
                     ARHGCRLHTHLAETHDETDFCLATFGRRPLDHLAEVGWLGPQTWLAHGIHFSADEIAR
                     LGAHGVGITHCPTSNMTLGSGQCRVLELEAAGSPVGLGVDGSASNDASNMMEEVRHAL
                     LLGRLTYGADRVTHLDALRWATQGSAACLGRADIGRLAVGACADLALFRLDQDLRFSG
                     GHDPLATLVLCGGGRADRVMVGGQWRVWDGAVVGLDVAQLRARHHALALGLFG"
     gene            complement(17413..18666)
                     /locus_tag="VFF98_07505"
     CDS             complement(17413..18666)
                     /locus_tag="VFF98_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63512.1"
                     /translation="MSTATLCAIDAAPPRAVATDRFSAPAVMAVIACGIGNIIGVTTI
                     VNATFSGFLVPVSGDLGLSRSAFTFVLTLISVLGLVAYPVSGRLIDRFGPRPVALAGN
                     LLFGLSVAGLGLVSAKLWVLYGLFALMGVLATLPGTVLFARVVSTWFFAHRGLVLGLS
                     GGVAFGIGGMIVPPFAEAVMQAWGWRAAYVGLGVLTIAVGQPVFWLWLKEAPRGGPAA
                     APAALTGLSRAEAMRTPAFWILLVGVAVGAGSTSSVITHMIPLAAERGFGFATALSAL
                     SALYAANAIWQTALGALLDRLGMARIAALFVLPGMAGAWLLNSAHSEGQVILGALLVG
                     LSAGTEYGLVPFCIPRYFGFRCYGEIYGVIFGMIMLLQGLAPFLMDLAYDLTGNYHPA
                     LAAFSFTLLGCAGVIAFLPRFGPRP"
     gene            complement(18663..>19114)
                     /locus_tag="VFF98_07510"
     CDS             complement(18663..>19114)
                     /locus_tag="VFF98_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU63513.1"
                     /translation="RTVFTRAPGERIPAVPPIFELGWNHTTLHALSRDPAISYIQADF
                     GPDWQRHVIAAAAELGEEAPLHLEMALIDGALCCFGISLLRFTSAERLEEIHQILRRH
                     GCTIFNPHTYILENGGMQVADPQQLAFRKEADPLGLCNPGKMPGWDV"
BASE COUNT         3055 a         6151 c         6714 g         3194 t
ORIGIN      
        1 gcgcacagcc cggcgcccgc cagcagcagg cttgcgggaa gacaggcgcg caaaagccgc
       61 gcgcgggcgg gcaaggtcgt tgtcatggcc ggttcctggg ctcctcgcct gtccggctgg
      121 gatgaaaaca cactgcttac cgcttccttc aaccacgtcc aatgcatgag cgcgcaaaag
      181 gtttccgcca tggtgcgata aaacggccgg ggcggacggc aaaaagccgt tgccgcaacc
      241 ctttgcgcgg gtctggcctg cctgtgccag cttggcgggc ggggagcaag ggtggccgga
      301 tcagccctgc cgttcagccc tgccgggcag cagggaccgg cagggcgtcg atggcagaag
      361 tgcccagccc gtggaggaag ctgtcgatgc acaggcccgc cgcacgggca tagcggcgcc
      421 acaggccggc cggcgcgcgg ccatggcgca caagctgggc gcgcagctgg cgttcggcca
      481 gcagggcggc atcgccggcg gtttcggcgc gcaaactgtc gagcgcggcc agcaccggca
      541 tccgccgcag cagatcgagc gcccgcgcgc tggcccgggc cgccggccgc tcgccgcagc
      601 gcaagggggc aaagccgcag cgctggctca gccagcccag cagggccggc gcttccagcc
      661 gcgcgcgcag ggtgctgatg ccatcacgcg ccagcgcatt gcgcgcgccc tgatgcgaca
      721 tccccgccag cagcgcgaga ttttccgcaa tggtgccgcc cagatccagc gcttccacct
      781 cgccggccac cagcgccgcg cgcagatggg cggccagcag cgtgtgccac agggcgggca
      841 ggctgtcggc cgggcgatgg ggggcagggg ccggcatcag ccccagcatc agccgcaaca
      901 cgcgggcgat ctcgcgccgg cgggtgtcgc tgatggcaag gtcggcgccg gtgcgaaagg
      961 cgctgtcatg ggcggtttgg gccagccggg tcaggtggca gggggccagc gggtcgatcg
     1021 cctggcgggc cggggcgctg accagcagtt ccagcatctg cgtgtattgg cccagaaagc
     1081 gggcgatatc atcggccagc gcggcagggg ccacgggcgg cggcagcagc gtgtgctgcc
     1141 acagcgcatc gaccaccgct accggctcgt cctcgatccg cagcgctgcg ggcttcaccc
     1201 atttgatccc ctcgggccgg ccatcgctca tgcgcagggc catgccggaa agggccagtt
     1261 cggccgcctc gccgtaattg tcgatgcgga tcagcgcatc ggtgcggatc tggcccccgc
     1321 gtggatccac aggggccagt tcgaaatgga agtccctgtc ccgccagggg cgctttaccg
     1381 tgccgcaggc atgggccgtg ctgcaggcat gggcagtgct gacaaggcgg ggcaggggtt
     1441 ccatccggga tcctcagttg aagggatagt agcggaagct caggaagccg atgttctcgc
     1501 ccacctgcgg catgatgcca ttggcatcgg cgaacagctg gcgctgggtg taggcatatt
     1561 gcaggcccac ctgcgcatgg ccgaaggagc cggaatagag cttgttccag aagccggtgg
     1621 tgagctgctg cacgcggcgg gtgttgcccg agcaggtgcc gccctcgatg aagcagccgg
     1681 cgttgttggc ggtgctggag gcattgccat agccgctggc gaagctgtag ccggtctcct
     1741 gctccttgcc ggcgaacagg tagagatcga gccgcttggt ggcatggaag gtggcgccgc
     1801 ccagcagcat gaactcgccc accgggtgga tcatgccatt ggcatcgaag gtgacatcac
     1861 ccagctgcgc cgaaccatag cgaccgatgc ccttgccgac gatgcccgag aactgcagat
     1921 ccagcttgcc cggcaccgcc tggaacaccg cgccgccgcc aaagccatag ccggcgcggt
     1981 tgatgttgta gccgccgttg agatgggcgg tgaaggtgcg cgacagggca aagccttcca
     2041 catgcagcga acggccagcc acagaaccct cataggccac cttgccgacg aaatcgggcg
     2101 tgccgttgag cgagagcgaa ttggccgtgt cgaagcccga gccggccgtg gcgttgtagg
     2161 tgatgaggct ggtgggcacg gtgccgaccg tggtggtggc cgcgttttcg gccgagacgg
     2221 ccagccacag cttgtggtcg gcggtgtcca ccgtcaggcg cagctggggc tggcgggccc
     2281 agaagaagcc ggggatatac tgcgcgtcga tctgcggcgg ggtcagctcg ttgcgcggcg
     2341 tgatgccctt ggtgttgagc gtgatgagcg accagttctg gccggccagc aaatggatgc
     2401 cgccgccatt gtgggcccag tcgatcgtgc cataaaggtg gcggatgcgc gggttgaagc
     2461 tgttgctttc gttcgaattg gcggtttgcg cgccgcccag gaagtcgaac tcgccataca
     2521 tggagagggt gacatccttg ttcaccttgc cctgcgccag gaacgacagg cggctctggc
     2581 gggccgagag gcggctttcg ctggcggtgc cggcgcggtt gttggggaag gggatggcaa
     2641 agctggtggc catgtcgttg ccctggaagc gctggcgata gatgccggcc atttccagga
     2701 agccgccggg cgtgatgtgc aggcccttga aatcggcata gcccttcttg tgctccttat
     2761 cgagcgcgac ggccacttcc tcccgggcgg cggtgtgggc ggccttgggt gcctgggcca
     2821 cttcatcgtg cagcgcgttc atctgcgctt ctgtctgggc cacctgggtc tgggtggtgg
     2881 ctgcactctg gcgctggctg gccagttcct gatgcagcgc gttcacttcg gcgcgcagtt
     2941 cggccagttc ctggttcacg ccgctgttct cggcgcgggg cggcgcggcg cgcttgtgag
     3001 cggcgcggtg atggaccgtt ttggtggcgg cctgcgccgg ggcgatgggc gcaaggaggc
     3061 tgccggcggc acaggccgcc agcaaggtgc tggcatattt catcggatat tccctcttgg
     3121 aggctggatt gcgcgccgga ggtatggggg tattatgtca tatctgttac taaatcgagg
     3181 ttgtttatgt attggttgca tgaaatttat ttaatgttca aaaaacgcac cgatttatgc
     3241 caggtgaaat aaatttaatc ttcgagggtt atttgaaggt gtaacgtgaa atttatttaa
     3301 tgttatctgg atttgatgaa ttccatgaag atcatttaac ttgattggca ggtgtggttg
     3361 caggattgaa ggggcgaaag gagcaccccg atgacgaact ctttcaatcg ttccgtgaac
     3421 cgctcgatcc gcaccctggc cctgggcggc ctgctggccc ttggcgcggg cggcgtggcc
     3481 caggcgcatg atgcccccta tcaggcccat ctctatggcc gggcgccctc ggcgggcctg
     3541 cattttgccg atctggaata tgccatgccc agcgatccgc tgacctggtc cagcgatccg
     3601 ggctgggaag cgcatcacac gccggcccgc ttcatgaccg atgcccaaag cgccctggct
     3661 gctgccgtgc ccgccggcct cagcgccgcc gaggccgccc agcgcctgca catggccggc
     3721 gcccattgcc gccagccggc cggcgccgag ctgagctgca cctacaaaac ggtcgagacc
     3781 ccgcgcacct attgggatgc ggtgacctgg acggtggcca tgccgctgac cgccgatggc
     3841 cgcgtggcca gcatgagcgt gacgcgcgac tggacacggc gctacctcta gagcaaagcg
     3901 gcttggcatg cggcagtgtg acggttagat gacaaatatg gcgcgcattc tggtggtcga
     3961 ggatgacccg gcaatggccg ggcgcattca gacgcggctc gaggctgaag gatatgaaac
     4021 ctgccacgcg gcaagcggcg gcgagggcct tgtgcatgtg caggatgggc ggttcgatgc
     4081 catcacgctc gatcgcatgc tgcccgatat ggatgggctg gagctgctgg gccggctgcg
     4141 cgcgcaaagc gtggcaacgc cggtgctgct gatcagctcg ctgggcgatg tggacgaacg
     4201 cattgccggc ctgcgcgccg gcggcgacga ctacatcacc aagcccttct cggccgacga
     4261 gatggccatg cgcgtggaag tgctgctgcg ccgtggccag ccgtccgcgg cagagccggc
     4321 cacctatgtg aacggcgatc tgcgcatgga tttcattgcc cgcaaggtgt ggctggccgg
     4381 cgagccggtg caattgctgc agaaggaatt cctgctgctg cagttcttcg tgcgccatcc
     4441 ggggcagacg atcacccggc ggatgatctt cgagcaggtg tggggctatc gcttcgatcc
     4501 cagcgacaat ctcatcaacg tccatgtcag caagctgcgg cgcaagctgg agcggcccga
     4561 tcacccctcg ccgatcgcca cggtgaaggg cgagggctat cggttcgatc cggtctagcc
     4621 gatgctgccc ctgctgcgca tgcgctggtc ggatatccgg cgcagcgcca ccttccggct
     4681 gaccacgctg ctgggctgtg tgttcctggt ggcggtggtg gcgctgctca gcctgaccta
     4741 tgtgctgacc gagaaggagc tggtcgcgcg caacgatcag gtggtctata aggaggcgcg
     4801 gcggctggcg gcggtgccgc gcgggcggat gaccgcggcg gtggacgcag cgattgccaa
     4861 taacgccagc gggctcaact atttcgggct gatcggcagc aacgggcagg tgctggccgg
     4921 caacatgccg caggtgccgc agctgctgcc gggcgagcct ttcgagatcc cggcgggggc
     4981 gggcgtgccg gtgccgctca gggtttatgt gttgccgctg gccgatggct gccggctggt
     5041 ggtggcgcgc gataacacca cgctggcgga cctgcgcgac cgggtgctgg cgatcaccgt
     5101 gggcagcggc ctgctggtgg cggtgtcggc ggcggtggtg ggcttctggt tgagcctggg
     5161 catgctgaag cgcatcggcc ggatgaccga caccgcccag catatcgctc gtggcgagct
     5221 gggccagcgc atgccggtgg ccggccgcca cgacgagctg gacctcatcg ccaccacggt
     5281 caatgccatg atggaagaga tcgagcggct gctgcagcag gtgaagggcg ccaccgatgc
     5341 catcgcccat gatctgcgca cgccgctggc tcatctgcgc caccgcctgc aggggctgag
     5401 cgagcgggag gaaaccgcct cgccgatcgt tgagcgggcg gtggaggagc tggatctggt
     5461 gctctcgcgc ttcaccgcgc tgctgcgcat ttccgagctg gaagtggcgg ggcggcgggc
     5521 cggctttggc ttgctcgatc cgatggcggt ggtggccgag gtgtgcgagc tgtatgagcc
     5581 gctggccgag gagcgcggca tccgcctcac cctctcgggc gatttcggcc aggtggtgga
     5641 gggcgaccgc ctgctgctga tcgaggcgat cggcaatctc gttgaaaatg ccatcaagtt
     5701 catcgcccgt ggcggcacgg tgcaattgca ggtggccgcc accggcgagc gcgtggcgat
     5761 cgaggtgcgc gacaatggcc cgggcattgc cgaggccgaa cgggcgctgg tgctgggccg
     5821 cttccaccgg ggggcgggcg ggcgccatgc gccgggatcg ggcctggggc tcagcctggt
     5881 ggcagcgatt gcccacctgc atggcttcgc gctcagcctg ggcgatgcgg cgcccggcct
     5941 tgtggtgcgc attatcgccc cgcgcgccgg cgagggcgcc tagctgcgtt tcatgtgttt
     6001 tttgtttaat gctgcgctgc atcacaataa ttttaattaa gttgcttcaa tcacctggct
     6061 gaatacatct gagactgact gtccatttcg cgaagtcggt ctttccgtca ggacttatgc
     6121 tcgaaattgt taagatagcg cttggcaagc catacacatt cgtggtcatg gccatcctta
     6181 tcgttctggg cggcagcatc gcctggatgg aaacccccac cgatatcttc cccgatatcc
     6241 gcatgccggt gatcgccgcc gtgtggacct acaacggcct gcagcccagc gacatgtcgg
     6301 gccgcgtggt ctatttctac gagcgccagc tcacctccac cgtcaacgac atcgatcata
     6361 tcgagagcca gtcgctgaac ggggtggggg tggtgaagat cttcttccac accggcgtcg
     6421 atatccgcac cgccaccgcc caggtgacag ccgcctcgca aacggtgctg aagcagatgc
     6481 cgccgggcat cacgccgccg ctgatcctga attacaacgc ctccaccgtg ccgatcttgc
     6541 agctggcgct gtcctcgccc agcctgtcgg aacagcagat cttcgatctg gggcagaaca
     6601 gcatccgcgc ctcgctggcc acggtgcagg gcgcggccat tccctcgccc tatggcggca
     6661 aggtgcgcca gatccaggtc gatctcgatc cggtggcgct ggccgcccgc catttgacgc
     6721 cggccgatgt gggcaccgcg ctggccgcgc agaaccagat catcccggca ggcgatgcgc
     6781 gcatcggcgg ctatgaatat gccgtgcggc tgaacaacag ccccgaggcc attgccgagc
     6841 tgaacaacct gccgatccgc acgctgaacg gcaccaccgt ttacatgcgc gatgtggcgc
     6901 tggtgcatga tggcgcgccg ccgcaaacca atgtggtgcg cgtggatggc cgccgcgccg
     6961 tgctgatgac ggtgctgaag gcgggcagtg cttcgaccat cgccattgtc gatggggtga
     7021 aggcgctgct gccgggcctg gcgcaaaccc tgccgagcga cctgcacatt gccgcgctgg
     7081 ccgatcagtc gctgttcgtg aagggggcga tctcgggggt ggtgcgcgag ggggcgattg
     7141 ccgctgcgct caccagcctg atgatcctgc tgttcctggg ctcgtggcgc tccacggtga
     7201 tcatcgccac gtccattccg ctggcggtgc tgggggcggt ggcggcgctg gcggccagcg
     7261 gcgagacgct gaacatcatg acgctgggcg gcctcagcct ggccgtcggc atcctggtcg
     7321 atgatgcgac cgtgacgatc gagaacatca actggcacct cgaacagggc aagggcgtgc
     7381 agcaggccat cctcgatggc gcggcgcaga tcgtgcagcc ggcgctggtg tcgctgctgt
     7441 gcatctgcat tgcctttgtg ccgatgttct ttctgggcgg cgttgccggc ttcctctttg
     7501 cgccgatggc caaggcggtg gtgtttgcca tgatggccag cttcgtgctc agccgcacgc
     7561 tggtgcccac catgggcaat tatctgctgc gcgcccatgc cagcgcacac accgccgatg
     7621 tgatggccag ccatgatgcc agcgccggcc ggccggcaac gcgcaacccc ctgcgccgct
     7681 tccagcatgg ctttgaaacc cgcttcgagg tggcccgccg ctggtatgtg gatctgctgg
     7741 ggctggcgct gggccatggc cgggcggtgg tgctgggcat ggcgggcgtg gtggcggtgt
     7801 cgctggcgct ggtgccgttc ctggggcgcg atttcttccc cgcggtcgat tccggccaga
     7861 tcagcctcca tgtccgcgcg ccggttggca cccgcatcga ggatagcgcc gccctgttcg
     7921 acagggtgga ggcgcagatc cgcagccagc tgccagccgg cgagatcgcc tcggtggtcg
     7981 ataatatcgg catgccggtc tcgggcatta acctggccta ttcgaacacc ggcggcatcg
     8041 gcccgcagga tggcgacatt ctggtgacgc tgaaggaggg ccatgcgccc accgcccaat
     8101 atgtgcgcca gctgcgcgcc cgcctgcccg gcgcttttcc gggcgtgtcc ttctcgttcc
     8161 tgccggccga tatcgtcagc cagatcctca acttcggggc gcccgcgccg atcgatgtga
     8221 tgatcaccgg ccccgatggc aaggcagacg aagcctatgc caaccgcctg ctggccgatc
     8281 tccagcatgt gccgggcctg gccgatgccc atgtgcagca ggccagcaac tatcccggcc
     8341 ttgccgtgga tgtcgatcgc agccaggcag accggctggg catcaccgaa agcgatgtca
     8401 cccgctcgct ctcggtcgat ctggccggca gcctgcagac cgcgcccgcc ttctggctca
     8461 acccgaagaa tggcgtgtcc tatccgatcg tggtgcaggc gccgcaatat cgcgccggca
     8521 cgctcagcga tctggccaat gtgccggtgg cggccagcgg cgctgccggc ctgcagacgc
     8581 tgagcgccat cggctccacc cggctggagc gcacgccggc ggtgatcgcc cattattcgc
     8641 tgcaacaggc gctggatatc tatgccacga ccgacgggcg cgatctgggc gcggtggcgg
     8701 gcgatctcca gaaggtgatc gatgcccaca aggctgatct gcccaagggc gcccatgtgg
     8761 acctgcgcgg ccaggtgcag acgatgaacg ccgccttttc cggcctgctg ctgggccttt
     8821 tgggcgccat cgcgctgatc tacctgctca tcgtcgtcaa cttccagagc tgggcggatc
     8881 ccgccgcgat catctcggcg ctgccggcgg cgctggcggg catctgctgg atgctgtttg
     8941 cctgccacac cacgctcagc gtgccggcgc ttaccggggc gatcatgtgc atgggcgtgg
     9001 ccacggccaa ttccgtgctg gtggtgagct tcgcgcggga acggctggcg gcttgcggca
     9061 atgcccttga ggcagcgctg gaggcgggtt ccacccgctt ccgcccggtg ctgatgacgg
     9121 cgctggcgat gatcatcggc atggggccga tggcgctggg ccttggcgaa ggcggcgaac
     9181 agaacgcgcc gctgggccgc gcggtgatcg gcggcctgct gtttgccacc atggccacgc
     9241 tggtcttcgt gccggtggtg ttcagcctca tccaccgcaa ttcctccctc ggagagacta
     9301 cccatgactg atttcagccc ttcgccggcc gccgcccccc gcggcctgcg caaggcgggc
     9361 atcgccatcg gcgctgtggc gctggtggtg gtgctcgccg gttcggcggt gcgtttccat
     9421 gccgctgccg atgcccgcaa tgtggccgca agccagggca cgccggccgt gcacctgctg
     9481 cagccccagc cgctgggcgc cagccagcag ctggtggagc ccggcgcgat cgccgcctgg
     9541 acagatgccc gcctgtttgc ccgcgtgccc ggttatgtgc atggctggta tgccgatatc
     9601 ggccagcgcg tggccgcagg ggcgccgctg ggccggatcg acacgcccga gctggacgag
     9661 cagatcaacg aggcgcgtgc cgccctggtg cgggccaggg ccgaggccgc gctggcgcgc
     9721 accacctcgg cgcgctggca ggatttgctc gtcagccact ccgtctcgcg gcaggaagcc
     9781 gacgagaagg ctgccgatgc cgccacccat gccgccggcg ttgccgaggc cgaggcgcat
     9841 ctgggccggc tgacggcgat gaaggccttt gccacgctgc gcgcgccctt tgccggcgtc
     9901 atcaccctgc gcaatgccga tgtgggcgat ctggtggggc cgggggccac gggccagcag
     9961 ccgatgttcg ccctggccga tgatcaccgg ctgcgcatct atgtcaacgt gccgcagcag
    10021 ttcactccgc tgatgcacgc cggccttacc gcccggttga ccgtgccggc ctggcccggc
    10081 cgcagctttg ccgcccgcgt gctcgatcag tcgggcgcga tcgacgggca gacgggcgcg
    10141 ctgaaagtgg aactggtggc cgacaatccc gatggcgcgc tgcggccggg cggctttgtg
    10201 caggtgggct ttgatctgcc gatgcctgcg ggccacctct cggtgccggt cagcgcgttg
    10261 atccagcgcg gcggcggcac gcgggtggca acggtggatg gcgccggcca tgcccatctg
    10321 ctgccggtga cgatcgggcg cgattttggc agcacggtgg aagtggtggc cggccttggc
    10381 gcgggcaccc cggtggtgga cagcccgccc gattcgctgg ccgagggcga ggcggtggac
    10441 gtggtggcca agggcggcgc ccatggctga gcgccgcttg ccccgctggc tggccacagg
    10501 tgtgctggcc accagcatgc tggccggttg caacatggcg ccggcctatc gcccgccgca
    10561 gctggccatg cccgcccatt tccgcgaaag cgccgggcca tgggcgccgg ccagcccggg
    10621 ccgcgcgcgg atcggcgcgc agtggtggca ggcgctgggt tcggccgagc tggacgggct
    10681 ggaagcgcgg ctgctggcca gcagcccgga tcttgccgcc gcccttgccc gccacgacat
    10741 cgcccgcgcg gcgctgacag aagcgcgcgc cagtgccttg ccgcaagtgg atgcgggcgc
    10801 ggatatcacc aacaaccgcc agtctgccaa ccgacccctg cgcggctctg gccagcccga
    10861 cgaatatgcc gccgacacgg ttggcgccgg cgccagctat gaactggatc tgtggggccg
    10921 ggtgcgcaac acggtggccg ccgcccatgc caatgccgat gccagcgccg atgatctggc
    10981 cgccgcccgc ctctcgctga cagccacgct ggcgcagacc tatatcgcgc tggtgggcac
    11041 cgatcgcgat tgcgcgattc tggctgatgc ggtggccgtt tatggccggg cggcggacgt
    11101 tacgcggcac cgcttcgagg aaggcatcgc cagcggcatc gatctgggcc gggccgatgc
    11161 ccagctggcc tctgcgcagg cggcgctggc cgaagcgcag ggccagcgcg cccggctgga
    11221 acatgccatt gccaccatgg tgggcacgcc tgccagcagc tttgccatcg ccccgcaggc
    11281 ggcaaggctg gcggtgctgc cggtgccggc cgccctgcca tcggccctgc tgcaggaccg
    11341 gcccgatgtc gccgcggccg aaaggcgcat gtatgccgcc aatcgcggca tcggtgtggc
    11401 gcgcgccgcg ctctatccgc agatcgatct catcggcggc ggcggcgtgc aggatacggc
    11461 gctggccggg ctgatggcgg cgcccaacct gttctggtcg gtggggccgc agatcagcct
    11521 gccactgttc gatggcggca agcgccgcgc ccgtgtgcgc caggcccagg gggaatggcg
    11581 ggcggccagc gcgctctatc gcggcgcggt gctgggcgcc attggcgagg tggaggatgg
    11641 gctggcgatg agccgccagc tgaccgaggc cagcgccgcg caggaaaggg cggccgccgc
    11701 ctccggcagc gcggccagcc ttgcctatgg cctgtatgtg aagggcgcgg ccagcctgct
    11761 cgacgtggtg accgcgcaga ccaccgcgct caccgccgcg cgcctggcga accagaccta
    11821 cacccgccgc ctgcagaccg gcgtcgatct ggtgcgggcg ctgggcgggg gcgtgatggc
    11881 gcccgccacc aaggcctgag cgccggccaa ggcctgaatc ccggccggcg ggatcagatg
    11941 tggatctcgc cggcctcctc ggcctggctt tcgaagaaca ggctggcgag gatggcagtg
    12001 gtggacgaca gcacgatgcc cgaatggagc agcggggtca gcgctgccgg catcgcctgg
    12061 aagaacttgt ccgacagcac cgggatcagg cccatcgcca ccgagacggc gatcgtggca
    12121 atgcgcccct gcgtcagctc cacgctggag aggatgcgga tgccggtggc tgcgatcatg
    12181 ccgaacatca tgaagcccgc cccccccagc acatagaccg gcagcgctgc caccagtgcg
    12241 gccagcttgg ggcacaggcc cagcatcatc agcaccaccc cgcccaccgc gcaaacatag
    12301 cgcgagcgca cgccggtgat ggaaagcagg ccgatgttct gggcatagga ggtgtagggg
    12361 aaggtgttga acacgccgcc gatcaccgtg cccagcgcat cggcgcgcag gccccgggca
    12421 aggtcggtgg tggtggccgg gcggcccacc agcgtgccgg cggccaggaa catgccgaag
    12481 ctctcgatca tggtgacgat catcacgaag cacatcgcca gcgagatcgg catgtcaaac
    12541 cgcggcatgc cgaactggaa gggcgtgacc agcgcaaacc acggtgcgct tgccaccggg
    12601 gcaaaatccg tttcgcccag ggccgttgcc agcaccgcgc cggcaatcac ccccagcagc
    12661 accgcgccat tgcgcaccag cccgcggcca tatttcatga agcccaggat gatcagcagc
    12721 gtggcaaagg caatgcccag atggagcggc gcgccatagc cgggctggga aggctggccg
    12781 ccggcggccc agtcgatcgc gatgcgcatc agcgacaggc cgatgatgag gatcaccgtg
    12841 ccggtgaccg tgggcgggaa gaagcgggtg agccggccaa cgaaaggcgc gatgagaaag
    12901 ccgaaaatgc ccgagacgat caccgcccca tagatcgttg gcaacaggcc catggccgcg
    12961 cccggcgccg gccctgccgc ctcgatcaac gccagcagcg ggctgaccga ggcaaaggtg
    13021 acgcccatca tgatcggcag gcggatgccg atgccgggca ggcccagcgt ctggatcagc
    13081 gtggcaaggc cggcggccac cagatcggcg ctgatcagca cggccagatg ttcgatggaa
    13141 agatgcatgg cgcgccccac caccagcggc accgccaccg cgcccgcata catcacgagc
    13201 acatgctgga tggcaaaggg cagcagcagg ccggccggcg gcacggcatc cacggcattg
    13261 gccccgtctt ccttcgccgc ctcggggtgt tccggggcgc cggtggtggc cagcggctcg
    13321 ggtgcggcgt tggcggtgtc tgcaaggata aggctcaacg cggggtactc cgaaagggca
    13381 ggtggccggg ccggcgaaaa gggcaaagcc ggcccggcca cctgatcagc aggccgtcag
    13441 aagttcacgc cgacacggcc gatgaactga cgcggcggca ccgccacgtc gaacacgccg
    13501 cccacgccga agttttcggc ataacgggtg gcgatatcct ggctgtcggt caggttggtc
    13561 acctgcagct ccagatacca cgggtgatgc gcgggctggt agtgcaggtt gaggttcatc
    13621 aggaactggc tgcccacgcg gtcccagggc gaattataca cgcgctgcca gaagggcgag
    13681 cgataggtcg tctgcagggc tgcatccagc ttgccatcgg ccacctcgga gcttttgctg
    13741 atggtgagcg aggaggagaa gttgggcacg cggcccagct ggtggccata gacatccgag
    13801 gcgtaatagg ccgcggtgcg gtcggcgatg tcgttgccgg cataggtgcc gccattggcg
    13861 cggttgatcg cctgagcaac ataggcatcc agcatgtgga catggctgtc caccttgctg
    13921 cccatccccg cggcattgcc atcgatgcgc cagccctcgg gcaggatcag cgagacttcg
    13981 gcttccacgc cataggtgtg gctcttgggg atgttggtca tgccgccctc atagggcagc
    14041 ggatcttccg aatcggcctg gaggttgcgg tagtcgttgt agaaggccga gaggttgagg
    14101 cgcagcttgc ggtcgaagaa ctcgttcttg ctgccgatct cgtaggcgcg cacgaattcg
    14161 tgcttgaagg cgctgggcac cacttcggag gccgggttga ggttggcgcc gcccggcttc
    14221 acgccggtgg agaagctggc atagagggtg gagtggcggg ccacgtcata ttccaccacg
    14281 gccttgccgg tcatcttgct gaattcgctg acgagggtgg caacggtgcc attggcagcg
    14341 gtgttggcgt ggtagctgtc gatgctgtag cgcaggccgc cggtcaggcg cagcttgtcg
    14401 gtgatgtgcc aggtggcctg gccatagccc gcccaggaat ggcgctgggc cagcgagacg
    14461 ctgacaaagg ccaggccata ggcgcccgac agataggact gcgcggcata gaggctggca
    14521 ttataggccg cggcatagct gatcgaatag ggaatgctct gctgggcggc ggtctgctgg
    14581 gtctcgaaca catattcgct gcccgagctg tgcaggtaga aggcgccgac gatccagtcc
    14641 accttgccgc cgggcttgga ggcgaggttg acttcctgcg tcaccgaatt ggtcgaccag
    14701 gagtggacgg ggatctcgtc cttcacgcca taataggacc aggcctgctc ggtgctgatc
    14761 atgtcgggcg aggtgcgcgc gttgtagtag acatactggt agctggtggt ggacttgacc
    14821 gtggcaaagc ccagatcata ggccacggtg gccgcggcaa tgcggctgcg ggtctgctcg
    14881 gggtcggcat agtcgttgct ggtggcgcgc acgcccgaga tcgaatcgac gatgttcttg
    14941 gcgcccacgc cgttgatgtc gttgctgtaa agctcgcccc aggcggtcac ggtcagcttg
    15001 tcggtgggct tccacagcgc cgagacgcgg cccgaccagg cgttttcgct gccgatatgg
    15061 ttggtgccgg gatagttggg ggcataatac cagccatcat gcgcctcgtg ctggccggtg
    15121 gcgcggatcg ccaggttctc ggtcagcggg atgttcagca tcgccttttc gcgattgtag
    15181 ttgtagctgc cgtagctggc ctcgccgctg ccggcaaaca cgcccagttt gggcaggttg
    15241 gtggtcacgt tgatggcgcc gccatcggcg ttctggccgt tcaccgtgcc ctgcggcccg
    15301 cgcagcactt ccagctggcc aagatcgaca aaggcgccgg tcagcgccac ggggctggcg
    15361 atatagacgc cgttctcgac aaaggcgacg ccgggctggg ccgacaggtt ctgcacgcca
    15421 tcatagccca cgccgcggat ggtgatgttg agcgggaagg cgccggcatc gctgacggtc
    15481 atgcccggca cctgcttgtc gagatcgaca gtggagcgga tgtcggcccg cgccagctgg
    15541 tcgcccgaaa gggtggagat ggcgatcggc gtcttttgca ggccggtgtt ggtcttctgc
    15601 gcggtaacgg tgatcatttc cagctgcggg gcggcggcat cggccgcttc ctcggcgcgg
    15661 gccgggctgc ccagagcaat cgtcagcgcc agggctgaaa cggacagggc ctgcgcgcag
    15721 gcgcgatggt atgtcatgaa atcccccatg aaaaaatcga aaatacccac ctttcttttc
    15781 ccgatgttcg gatcgggttg tttttggcat cctcggagaa ttttttggaa tttatgattt
    15841 tatttttatc atcgtgctga agatttggag aatgagataa aaatcgcctg atattcgggt
    15901 ttttggtgca atcgatgtgg tgctgcgata ataggcctcc cacagtgttc cataaagaag
    15961 aaaatttttc ccataacgtt ccaatttttg gaacatcaac cgccggccgg ccttcccccg
    16021 ccccggcgca ggagtgtggc aagatgcgta tctggatcag caacccccag gcgattttcg
    16081 gcgaggcctg tgcaggcggg ctggtggtgg ccgatggccg cattgccgag ctggtgcccg
    16141 ccggcgcccg gcccacgggc ccggtggacg aagtgatgga tgccagcggc catgtcgtgc
    16201 tgccgggcct catcaacacc caccaccatt tctaccagac gctgacccgc gcccatccgg
    16261 cggcgatcaa tcaggcgctg ttcggctggc tgcaggcgct ctatcccatc tggcgccggc
    16321 tcagccccga ggcgctggaa ctggcgagcg aggcggcgat ggcagagctg ctgctgtcgg
    16381 gcgtcaccac tgcggccgat catcactatg tgttcccggc cgggctggaa gcggcgatcg
    16441 acatccaggt ggaagtggcc cgccggctgg gcctgcgggt tacgctcacg cgcggctcga
    16501 tgagcctgtc caccgccgat ggcggcctgc cgccccccga agtggtgcag gatcacgaca
    16561 ccatcctggc cgacagcgag cggctgatca cccgctatca cgatgccggg gatggggcga
    16621 tggtgcagat cgcgctggcg ccgtgctcgc ccttctcggt caccccgcag ctgatggccg
    16681 acagcgccgc gctggccgcc cgccatggct gccgcctgca cacccatctg gccgaaaccc
    16741 acgacgagac ggatttttgc ctcgccactt tcggtcgtcg cccgctcgat catctggccg
    16801 aggtgggctg gctggggccg cagacctggc tggcccatgg catccatttc tcggctgacg
    16861 agatcgcccg ccttggcgcc catggcgtgg gcatcaccca ttgccccacc tcgaacatga
    16921 cgctgggctc tggccagtgc cgcgtgctgg agctggaagc ggcgggcagc ccggtggggc
    16981 tgggggtgga tggctcggcc tccaacgatg ccagcaacat gatggaggaa gtgcgccatg
    17041 ccctgctgct gggccggctg acctatggcg ccgacagggt gacgcatctc gatgcgctgc
    17101 gctgggccac gcaagggtct gccgcatgcc tgggccgggc ggatatcggc cggctggcgg
    17161 tgggggcctg cgctgatctg gcgctgttcc ggctggatca ggatctgcgt ttctcgggcg
    17221 ggcatgatcc gctggccacg ctggtgctgt gcggcggcgg ccgggccgac cgggtgatgg
    17281 tgggcggcca gtggcgcgtg tgggatgggg cggtggtggg ccttgatgtg gcgcagctgc
    17341 gcgcgcgcca ccatgcgctg gcgctcggcc tgttcggctg agggccaggg cgcggtcatc
    17401 ttcctgccgc catcagggcc ggggcccgaa gcgcggcagg aaggcgatca cgccggcgca
    17461 gcccagcagg gtgaaggaga aggccgccag cgccgggtgg taattgccgg tgaggtcgta
    17521 ggcgaggtcc atcaggaagg gggccagccc ctgcagcagc atgatcatgc caaagatcac
    17581 gccatagatc tcgccatagc agcgaaagcc gaaatagcgc gggatgcaga acggcaccag
    17641 gccatattcc gtgcccgcgc taaggcccac cagcagcgcg cccaaaatca cctgcccctc
    17701 gctgtgggcg ctgttcagca gccaggcgcc agccatgccg ggcagcacga aaagggcggc
    17761 aatgcgcgcc atgcccagcc ggtccagcag ggcgcccagc gctgtctgcc agatggcatt
    17821 ggcggcatac agcgccgaca gcgccgatag cgcggtggca aagccaaagc cgcgctcggc
    17881 cgccagcggg atcatgtggg tgatgaccga ggaggtggag ccggccccca ccgccacccc
    17941 caccagcagg atccagaaag ccggcgtgcg catcgcctcg gcgcgggaaa ggccggtgag
    18001 ggcagccggc gctgcggcgg gccctccgcg cggcgcctcc ttcagccaca gccagaacac
    18061 tggctggccc acggcaatgg tcagcacgcc cagccccaca taggccgcgc gccagcccca
    18121 ggcctgcatc accgcctcgg caaagggcgg cacgatcatc ccgccaatgc caaacgccac
    18181 cccgcccgac aggcccagca ccagcccgcg atgggcaaag aaccaggtgg acaccacgcg
    18241 cgcaaacagc accgtgcccg gcagcgtggc cagcactccc atcagcgcaa acaggccata
    18301 cagcacccac agcttggccg acaccagccc cagcccggca accgacaggc caaacagcag
    18361 attgcccgcc agcgccaccg gccgcggccc gaaccggtct atcagccggc ccgagaccgg
    18421 ataggccacc agccccagca cgctgatcag cgtgagcaca aaggtgaaag ccgagcgcga
    18481 cagcccgaga tcgcccgaca ccggcacgag aaagccggag aaagtggcgt tcacgatcgt
    18541 cgtcacgccg atgatgttgc cgatgccgca agcgatcacc gccatcaccg cgggcgcgct
    18601 gaaacggtct gttgccaccg cgcggggcgg ggcggcatcg atggcgcaga gggtggcggt
    18661 gctcacacgt cccagccggg catcttgccg gggttgcaca ggcccagcgg atcggcctcc
    18721 ttgcggaagg ccagttgctg cggatcggcc acctgcatgc cgccgttttc cagaatgtag
    18781 gtgtgcgggt tgaagatggt gcagccgtgg cggcgcagga tctggtggat ctcttccagc
    18841 cgctcggcgc tggtgaagcg cagcagcgat atgccaaagc agcacagcgc gccatcgatc
    18901 agcgccattt ccagatgcag cggcgcttcc tcgcccagtt cggcggcggc ggcgatcaca
    18961 tggcgctgcc agtccggccc gaaatcggcc tggatatagc tgatggcggg atcgcggctg
    19021 agggcgtgca gcgtggtgtg gttccagccc agctcgaaga tcggcggcac ggcggggatg
    19081 cgctcgcccg gcgcgcgggt gaacacggtg cgcc
//
LOCUS       DASRYE010000138         8481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_113731,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000138 DASRYE010000000
VERSION     DASRYE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113731"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..3430)
                     /locus_tag="VFF98_07515"
     CDS             complement(272..3430)
                     /locus_tag="VFF98_07515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZU63514.1"
                     /translation="MSQPAFFAEPVAASAFSFLRGASAPADMVARAHALGLAGLGLAD
                     RNTVAGTVRAHVAWQGLGGAQSGLRLIVGARLVFADGTPDIAAYPVDRAGWGRLTRLL
                     TLGNRRAEKGDCTLFLADLLGHAEGLALVAMAGDAPLLGALGEAAAHLWLAATMPRGG
                     ADARQLATRMALAQAAGVPLIATNDPLYARPQCRPLHDVMTCIREGLRVQSAGRRLAP
                     HAERHLKPPAEMARLFAACPRALAATGDLFDAIGFTLDALRYEYPHEPVPPGHSPQGW
                     LEELVARGGAARFPAGLPPAYRATLAEEFRLIAAKGYAPYFLTVHDIVAHARGLSPPI
                     LCQGRGSAANSLVCYFLGITPIDPVREKLLFSRFLSEERDEPPDIDVDFEHERREEVI
                     QYIYRRYGRERAGIAATTIHYRQRSALREVGRALGLSEDVTARLSGTVWGSRGGPLPA
                     ARLAEAGFAATNPEIARLADLVGQLVEMPRHLSQHVGGFVLTRGRLDELVPIHNAAME
                     ARTFIEWDKDDIDALGLMKVDVLALGMLTCIRKSFELMRAHGLGDYTLASVPREDAAT
                     YRMLQAADSIGTFQVESRAQMAMLPRMKPRCLYDLVIQVAIVRPGPIQGGMVHPYLRR
                     RNGEEKVTYPGPPGEMREVLGKTLGVPLFQEQAMKLAIVAAGFTPAEADGLRRAMATF
                     RRHGTIHHYEIKLVEGMVARGYTRDFAERCFEQIKGFGDYGFPESHAQAFAWLAYVSS
                     WLKCHYPAVFTAALLNSQPMGFYAPAQLVADARAHGVAVRPPCVLASGWDNGLEGARI
                     LRLGLRQIEGLRREWAEAIVAARGQAPFAGLEDVARRAALPARAMALLADADAFAALG
                     HGRRAAGWDARRIAPRQLSLFAATGAPELAAEPGPALPPMPARQEVAEDYRSQRLSLR
                     GHPVQFLRATLQAQGLCSCAQVNAARDGARLRTAGLVLVRQRPGKGNAVFLTIEDETG
                     VVNALLWARDFEAQRAAVMAARLMEIEGHAQRSKEGVVHLMAARITDRTALLLGSDAA
                     PAPPRASHPRDMRLLPKSRDFH"
     gene            complement(3427..4866)
                     /locus_tag="VFF98_07520"
     CDS             complement(3427..4866)
                     /locus_tag="VFF98_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZU63515.1"
                     /translation="MLVALVGQARRLAAVNPAAAALGLAPGLPLADARARFPDLAAAP
                     ADPVADARLLERLATRMAAFSPLVALDPPDGLLLDITGAAHLFGGEAAMAAAVVAAAV
                     AACGSAARPALADHAAAARALARFGAGAAQDVRCLPVAALEPGEEVLAALASAGLVWL
                     GDLAARPMAPLAARFGEGLVRALRAILGEVAPPLIPWRTLPPIHEEARFAEPIARTQD
                     ALAVIEALLLRAGTTLQARAQGGRRFIVELERGDGLRRRLAVETGQPTQAPAPVMRLL
                     RERIETLADPLDPGFGFDTIRLAVGRTQSLPPRQIDTAGTAAEAGDISALIDRLSLRL
                     GADAVCRLAPVDTHVPEAAQRLLPAREAPPQAWDLAPAALPRPLLLFDPPQPVAVIAS
                     VPDGPPLRFRWQGQLHEVRLAEGPERIAPEWWHAPDGQQGAATGPGHALSRDYYRVED
                     AGGRRFWLLRHGLFGETEAPLWYVHGLCA"
     gene            complement(4883..5665)
                     /locus_tag="VFF98_07525"
     CDS             complement(4883..5665)
                     /locus_tag="VFF98_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63516.1"
                     /translation="MPPAQERPCGPADLAGFPSAAGLAGFPGAADLAGFPGLAPARLH
                     EIHAEARDWGAALAFALGAAGGGTGPVVLVRRQGRAALQLCAEGLVGLGLDPARLLLV
                     AARDERALLRAGLDAARCPGLAAVVLESWGPLPLYDLTASRRLVLAAERCGAAVLLLR
                     GQAPPRASAAHTRWQIRAAPSAPLAARAPGPAAIEAELLRLRGGPAGRRWQLEWDETH
                     AIFRTGQAPFPTSAGGGGAALSGAVVSLAGLRQGPAGGRRAA"
     gene            5844..7157
                     /locus_tag="VFF98_07530"
     CDS             5844..7157
                     /locus_tag="VFF98_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HZU63517.1"
                     /translation="MAHRPPPQQPLSLHVPEPPFRPGDKVDYSFLQIPPVGAQPRPAE
                     TCPPAETHPLCHDLIRVLDEAHQAQGPWNPGLDAETLRRMLRVMALTRLLDDRLYRAQ
                     RQGKTSFYMKSTGEEACAVGAAFALAPDDMLFPAYRQQGMLIARGYPLVEMINQVYSN
                     RADRLKGRQMPVMYSSRAHHVFTISGNLATQFPQAVGWAMASAIKGDSRIASGWIGEG
                     SSAEGDAHAALLFAAVFNAPVVLNIVNNQWAISSCSGFAGAERAPFAARAAGYGIAGL
                     RVDGNDALAVYAATRWAANRARANGGPTLIEHFTYRAEAHSTADDPTAYRSAQERAEW
                     PLGDPVTRLKRHLVALGEWSAEAHAAMDSALVEEVSAALREAEKNGTLGHGLHHPFHT
                     MFEDVFETLPWHLEEQAEQAIRERRIKWPDWQDQHQGRGGKEERG"
     gene            7154..8206
                     /locus_tag="VFF98_07535"
     CDS             7154..8206
                     /locus_tag="VFF98_07535"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZU63518.1"
                     /translation="MIEEGPVSLQQAPSRRLTMIQAINEALDLMLERDPDVVIMGEDV
                     GYFGGVFRATAGLQKKYGKTRVFDTPINECGIIGAAVGMAAYGLRPVPEIQFADYIYP
                     GFDQLVSEAARLRYRSAGEFVAPITVRAPFGGGIFGGQTHSQSPEALFTHVAGLKTVV
                     PSTPHDAKGLLIAAIEDNDPVLFFEPKRLYAGPFDGYYEAPARAWADHPDSAVPEGYY
                     ALPLGKARLVREGAEMSVLAYGTMVHVAEAVLAARGVAADLLDLRSLVPLDIEAITAS
                     VAKTGRCLIVHEATRTGGFGAELAALVQERCFYHLEAPIERVTGFDTPYPHSLEWAYF
                     PGPVRIGEAVDRLRSA"
     gene            8208..>8481
                     /locus_tag="VFF98_07540"
     CDS             8208..>8481
                     /locus_tag="VFF98_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZU63519.1"
                     /translation="MGRYTFRLPDIGEGLAEAEIVCWHVGVGDRVAADAPLVDMMTGK
                     ATVEMESPVAGRVLALAGAPGDSVRVGAALAVLEVAGEGDGEAPEPA"
BASE COUNT         1313 a         3047 c         3017 g         1104 t
ORIGIN      
        1 cccccagcct tgccggttag ccagttccag ccgcagggca tcgggcgcgg gcgctgcggc
       61 cgatgccatt gccggcaggg ttagcgccag cagggtcagc gcagcaagca gcgcccatgg
      121 catgcgggaa aagcgggcgg ggcagtgtcg catggtctgt ctcgtcgttt gccggtgatg
      181 ctaggtgggc gatgcggggc gggcgatgtg gggcggtgta tggcacggga aaggtaagcg
      241 caggctgaac cgccgccatc agcgcccgga ttcagtgaaa atcgcgcgat ttgggcagca
      301 ggcgcatatc gcgcggatgg ctggcgcggg gcggggcggg cgcggcatcg ctgcccagca
      361 gcagcgcggt gcgatcggtg atgcgcgcgg ccatcagatg caccacccct tccttgctgc
      421 gctgcgcatg gccttctatc tccatcagcc gggcggccat cacggcggcg cgctgggcct
      481 cgaaatcgcg cgcccacagc agggcgttca ccacccctgt ctcatcctcg atggtgagga
      541 acacggcatt gcccttgccc ggccgctggc gcaccagcac cagccctgcg gtgcgcaggc
      601 gggcgccatc gcgggcggcg ttcacctggg cgcagctgca caggccctgc gcctgcaagg
      661 tggcgcgcag aaactgcacc ggatggcccc gcagcgagag gcgctggctg cgataatcct
      721 ccgccacttc ctgccgggcg ggcatgggcg gcagggcggg gccgggctct gcggccagtt
      781 cgggcgcgcc tgtggcggca aacaggctga gctggcgcgg ggcgatgcgt cgcgcatccc
      841 agccggccgc ccgccggccg tggcccagcg cggcaaaggc atcggcatcg gccagaaggg
      901 ccatggcgcg cgccggcaga gcggcacggc gggccacatc ctccagcccg gcaaagggcg
      961 cctgcccccg cgcggcgacg atcgcctcgg cccattcccg gcgcaggccc tctatctggc
     1021 gcaggcccag ccgcaggatg cgcgcgcctt ccagcccatt gtcccagccg ctggccagca
     1081 cgcaaggcgg gcgcaccgcc accccatggg cgcgcgcatc ggccaccagc tgggccgggg
     1141 catagaagcc catcggctgg ctgttcagca gggcggcggt gaacacggcc ggataatggc
     1201 atttcagcca gctcgacaca taggccagcc aggcaaaggc ctgcgcatgg ctttcgggga
     1261 agccataatc gccaaagccc ttgatctgct cgaaacagcg ctcggcgaaa tcgcgcgtgt
     1321 agccgcgcgc caccatgcct tccaccagct tgatctcgta atggtggatg gtgccgtggc
     1381 ggcggaaggt ggccatggcg cggcgcagcc cgtcggcctc ggccggggtg aagccggccg
     1441 ccacgatcgc cagcttcatc gcctgttcct gaaacagcgg cacgcccagc gttttgccca
     1501 gcacttcgcg catttcgccg ggggggccgg gataggtcac cttttcctcg ccattgcgcc
     1561 gccgcagata gggatgcacc atgcccccct gaatgggccc gggccgcacg atcgccacct
     1621 ggatcaccag atcatacagg cagcgcggct tcatgcgcgg cagcatcgcc atctgcgccc
     1681 ggctttccac ctggaaggtg ccgatgctgt cggccgcctg cagcatccga taggtggccg
     1741 catcctcgcg cggcacgctg gccagcgtgt aatcgcccag cccatgggcg cgcatcagct
     1801 cgaaactctt gcggatgcag gtgagcatgc ccagcgccag cacatccacc ttcatcagcc
     1861 ccagcgcgtc gatatcgtcc ttgtcccatt cgatgaaggt gcgcgcttcc atcgccgcat
     1921 tgtggatcgg caccagctcg tccagccgcc cacgggtcag cacaaagccg ccgacatgct
     1981 gcgagaggtg gcgcggcatt tccaccagct gacccaccag atcggcgagg cgggcgattt
     2041 ccgggttggt ggcggcaaag ccggcctcgg ccagccgcgc ggccggcagc gggccgcccc
     2101 tgctgcccca caccgtgccc gagaggcgcg cggtcacatc ctcgctcagc cccagcgccc
     2161 ggcccacctc gcgcagggcg cttctctggc ggtagtggat ggtggtagcg gcaatcccgg
     2221 cccgctcgcg gccatagcgg cgatagatgt actggatcac ttcctcgcgc cgctcatgct
     2281 cgaaatcgac atcgatatcg ggcggctcgt cgcgctcttc cgagaggaag cggctgaaca
     2341 gcagcttctc gcgcaccgga tcgatcggcg tgatgccaag gaaatagcac accagcgaat
     2401 tggccgcgct gccccgcccc tggcacagga tgggcggcga caggccgcgc gcatgggcaa
     2461 cgatatcatg cacggtgagg aaatagggcg catagccctt ggcggcgatc aggcggaatt
     2521 cctcggccag cgtggcgcga taggccggcg gcaggccagc gggaaaacgc gccgcgccgc
     2581 cgcgcgccac cagttcctcc agccagcctt gcgggctgtg gccggggggc accggctcat
     2641 gcggatattc atagcgcagc gcatccagcg tgaagccgat ggcatcgaac agatcaccgg
     2701 tggccgccag cgcccggggg caggcggcaa acaggcgggc catttcggcc ggcggtttca
     2761 gatgccgctc ggcatggggg gcaaggcggc ggccggcgct ctgcacgcgc agcccctcgc
     2821 ggatgcaggt catcacatcg tgcagcgggc ggcattgggg gcgggcatac agcggatcgt
     2881 tggtggcgat cagcggcacg ccggcggcct gagccagcgc catgcgcgtg gccagctggc
     2941 gggcatcggc gccaccgcgc ggcatggtgg cggccagcca cagatgcgcc gcagcctcgc
     3001 ccagcgcgcc cagcaggggg gcatcgcccg ccatcgccac cagcgccagc ccttcggcat
     3061 ggcccagcag atcggccagg aacagcgtgc aatcgccctt ttcggcgcgg cggttgccca
     3121 gcgtcaacag gcgggtaagg cggccccagc cggccctgtc caccggatag gcggcgatat
     3181 cgggcgtgcc atcggcaaac accagccgcg cgcccacgat cagccgcaag ccgctttggg
     3241 cgccgcccag cccctgccag gccacatgcg cgcgcaccgt gccggccacc gtgttgcgat
     3301 cggcaaggcc aaggccggcc agccccagcg catgggcgcg cgcgaccatg tcggccggcg
     3361 ccgaggcccc gcgcaggaag ctgaaggcgc tggccgccac aggctcggca aagaaggcgg
     3421 gctggctcat gcgcaaagcc cgtgcacata ccagagcggc gcctccgtct cgccaaacag
     3481 gccatggcgc agcagccaga agcgccgccc cccggcatcc tccacccggt agtaatcgcg
     3541 gctgagggca tggccgggcc cggtggccgc gccctgctgg ccatcgggcg cgtgccacca
     3601 ttcgggggcg atccgctcgg gcccttcggc caggcgcacc tcatgcagct gcccctgcca
     3661 gcgaaagcgc agcggcggcc catcgggcac gctggcgatc accgccacag gctggggcgg
     3721 atcgaacagc agcagcgggc ggggcagggc cgccggcgcc agatcccagg cctgcggcgg
     3781 cgcctcgcgg gcgggcagca ggcgctgggc ggcctcgggc acatgggtgt cgacaggggc
     3841 cagccggcac acggcatcgg cccccagccg caggctcagc cggtcgatca gggcgctgat
     3901 gtcgcccgcc tcggcggcgg tgccggcggt gtcgatctgg cggggcggca ggctttgcgt
     3961 gcggcccacg gcgaggcgga tggtatcgaa gccgaagccg gggtcgagcg ggtcggccag
     4021 cgtctcgatc cgctcgcgca gcaggcgcat caccggggct ggcgcctgtg tgggctggcc
     4081 ggtttccacc gccagccggc ggcgcagccc atcgccccgc tccagctcga cgataaagcg
     4141 ccgcccccct tgcgcgcggg cctgcagggt ggtgccggcg cgcagcagca gcgcctcgat
     4201 caccgccagc gcatcctggg tgcgggcgat gggctcggca aagcgggcct cctcgtggat
     4261 ggggggcagc gttcgccagg ggatcagcgg cggcgcaacc tcgcccagaa tggcgcgcag
     4321 ggcccgcacc agcccctcgc caaagcgggc ggccagcggc gccatgggcc gcgcggccag
     4381 atcgcccagc cacaccagcc cggcgctggc cagggcggcc agcacctcct cgcccggctc
     4441 cagcgcggcc accggcaggc agcgcacatc ctgcgccgcc cccgcgccaa aacgggccag
     4501 cgcgcgggca gcagcggcat gatcggccag cgcgggccgc gcggcgctgc cgcaggccgc
     4561 caccgctgcc gccaccaccg ctgccgccat cgccgcctcg ccgccgaaca gatgcgccgc
     4621 cccggtgatg tcgagcagca gcccgtcggg cggatccagc gccaccagcg gcgagaaggc
     4681 cgccatgcgc gtggccagcc gttccagcag gcgggcatcg gcgacaggat cggccggcgc
     4741 tgccgccaga tcgggaaagc gcgcccgcgc atcggccagc ggcaggccgg gggccaggcc
     4801 cagcgcggca gcggccgggt tcaccgcggc cagccgccgc gcctggccca ccagcgccac
     4861 cagcacgaag gggcgcgctt cctcaggcgg cgcggcgccc gccagccggg ccctggcgca
     4921 gcccagccag ggaaaccaca gcgccagata gcgccgcccc gccgccgccg gcgctggtgg
     4981 ggaagggggc ctggcccgtg cggaagatgg catgtgtctc gtcccattcc agctgccagc
     5041 gccggcctgc cggcccgccg cgcaggcgca gcaattctgc ctcgatagcc gccgggcccg
     5101 gcgcgcgggc ggccagcggc gccgagggcg cggcgcggat ctgccagcgc gtgtgggcgg
     5161 cgctggcgcg cgggggcgcc tgcccgcgca gcagcagcac ggccgcgccg cagcgttcgg
     5221 ccgccagcac cagccggcgg ctggcggtca gatcatagag cggcagcggc ccccagcttt
     5281 cgagcaccac cgcggcaagg cccgggcagc gcgcggcatc gaggccggcg cgcagcagcg
     5341 cccgctcgtc gcgcgcggcc accagcagca gccgcgccgg atcaaggccc agccccacca
     5401 gcccttcggc gcagagctgc agcgccgcgc gtccctgccg gcgcaccagc accaccggcc
     5461 cggtgccacc accggccgcg ccaagggcaa aggccagcgc cgcgccccag tcgcgcgctt
     5521 cggcgtggat ctcgtgcagg cgggcggggg ccaggccggg gaagccggcc aaatcagcag
     5581 cgccggggaa gccggccaag ccagcagcgc tggggaagcc ggccaaatca gcagggccgc
     5641 aggggcgttc ctgtgcgggc ggcatggggc aatctcctga gcgaatcggt gaacatgttt
     5701 ccattatgtt ccatttgggc ggggcctgtc cacgcagcag atcccgcgcg gcccgcctct
     5761 ctcgttttgc cgttgacacc ggcccgcagc tggcctgaaa tgccccgtct gggaagagaa
     5821 gctgaaaggc cgaagactgg accatggcgc accgcccgcc cccgcagcag cccctgtcgc
     5881 tgcatgtgcc cgagccgccc ttccggcccg gcgacaaggt ggattacagc ttcctgcaaa
     5941 tcccccccgt cggcgcccag ccccgcccgg ccgaaacctg ccccccggca gaaacccacc
     6001 ccctgtgcca cgatctgatc cgcgtgctgg acgaggcgca ccaggcgcag gggccctgga
     6061 accccgggct cgatgccgaa acgctgcgcc ggatgctgag ggtgatggcg ctcacccgcc
     6121 tgctcgatga ccgcctctat cgcgcccagc gccagggcaa gaccagcttc tacatgaaat
     6181 ccaccggcga ggaggcctgc gccgtgggcg ccgcctttgc cctggcgcca gacgacatgc
     6241 tgtttcccgc ctatcgccag cagggcatgc tgatcgcgcg gggctatccg ctggtggaga
     6301 tgatcaacca ggtctattcc aaccgggcag accggctgaa gggccggcag atgccggtga
     6361 tgtattccag ccgggcgcat catgtcttca ccatcagcgg caatctggcc acgcagtttc
     6421 cgcaagcggt gggctgggcg atggccagcg ccatcaaggg cgacagccgc atcgcctcgg
     6481 gctggatcgg cgagggctcc agcgcggaag gcgatgccca tgccgcgctg ctgtttgccg
     6541 ccgtgttcaa cgcgccggtg gtgctcaaca tcgtcaacaa ccagtgggcg atctccagct
     6601 gctcgggctt tgcgggggcg gaaagggcgc cctttgccgc ccgcgcggcc ggctatggca
     6661 tcgccgggct cagggtggat ggcaatgatg cgctggcggt ttatgcggcc acgcgctggg
     6721 cggccaaccg ggcgcgggcc aatggcggcc ccacgctgat cgagcatttc acctatcgcg
     6781 ccgaggccca ttccaccgcc gatgacccca ccgcctatcg cagcgcgcaa gaacgcgccg
     6841 aatggccgct gggcgatccg gtaacgcggc tgaagcggca ccttgtcgcg ctgggcgagt
     6901 ggtcggcaga agcgcatgcg gcgatggaca gcgcgctggt cgaggaagtg tcggccgccc
     6961 ttcgcgaggc ggagaagaac ggcacgctgg gccatggcct gcaccatccg ttccacacca
     7021 tgttcgagga tgtgttcgaa accctgccct ggcatctgga ggagcaggcc gaacaggcca
     7081 tccgcgagcg gcgcatcaaa tggcccgact ggcaggacca gcaccagggc aggggcggca
     7141 aggaggagag gggatgatcg aggaaggccc ggtatcgctg caacaggccc ccagccgccg
     7201 cctcaccatg atccaggcga tcaacgaggc gcttgatctc atgctggagc gcgatcccga
     7261 tgtggtgatc atgggcgagg atgtcggcta tttcggcggg gttttccgcg ccacggccgg
     7321 ccttcagaag aaatacggca agacccgcgt gttcgacacg ccgatcaatg aatgcggcat
     7381 catcggcgcg gcggtgggca tggccgccta tggcctgcgc ccggtgcccg aaatccagtt
     7441 tgccgattat atctaccccg gcttcgacca gctggtgtcg gaagcggcgc ggctgcgcta
     7501 tcgctcggcg ggcgaatttg tcgcgccgat caccgtgcgc gcgccctttg gcggcggcat
     7561 cttcggcggg cagacccaca gccagagccc ggaagccctg ttcacccatg tcgccgggct
     7621 gaaaacggtg gtgcccagca cgccgcatga tgccaagggc ctgctgatcg ccgccatcga
     7681 ggacaacgac ccggtgctct tcttcgagcc caagcgcctc tatgccggcc ccttcgacgg
     7741 ctattacgaa gcgcccgccc gcgcctgggc cgatcacccc gacagcgccg tgcccgaagg
     7801 ctattacgcg ctgccgctgg gcaaggcgcg gctggtgcgc gaaggggcag agatgagcgt
     7861 gctggcctat ggcacgatgg tgcatgtggc agaagcggtg ctggcggcca ggggcgtggc
     7921 ggcggatctg ctggatctgc gcagcctcgt cccgctcgac atcgaggcga tcaccgcatc
     7981 tgtcgccaag accggccgct gcctcatcgt gcacgaggcc acccgcaccg gcggctttgg
     8041 cgcggaactg gccgccctgg tgcaagagcg ctgcttctac cacctcgaag cgccgatcga
     8101 gcgcgtcacc ggcttcgaca cgccctatcc ccacagcctc gaatgggcct atttccccgg
     8161 ccctgtgcgc atcggcgagg cggtggaccg cctgcggagc gcctgagatg ggccgctaca
     8221 ccttccgcct gcccgatatc ggcgaggggc tggccgaggc ggagatcgtc tgctggcatg
     8281 tgggcgtggg cgatagggtg gccgccgatg ccccgctggt ggacatgatg accggcaagg
     8341 caacggtgga aatggaaagc ccggtggccg gccgggtgct ggccctggcc ggcgcgccgg
     8401 gcgacagcgt gcgcgtgggc gcggcgctgg cggtgctgga agtggcgggc gagggggacg
     8461 gtgaggcgcc cgagccggct c
//
LOCUS       DASRYE010000139        10460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_78480,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000139 DASRYE010000000
VERSION     DASRYE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78480"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFF98_07545"
     CDS             complement(<1..591)
                     /locus_tag="VFF98_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63520.1"
                     /translation="MEFAFTEEQEMIAETVNGFFAEHATSERTRAAMAGEGIDRALWG
                     AFCTELGMAGVAVPEALGGAGLGLVELAIVAQAAGGQVAAVPLLGHAMVARALAAGGS
                     AQQQAQWLPQLLSGAAIAAFAQAETVTEAGGALSLVADFVAHGALADLFLVAAGKGAW
                     LVERNAAGLSVSAHVTMDQTRPLATLALAGTRAQELA"
     gene            complement(596..1498)
                     /locus_tag="VFF98_07550"
     CDS             complement(596..1498)
                     /locus_tag="VFF98_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63521.1"
                     /translation="MGICDNRTVIITGAAGGLGRAYALAFAAEGACVVVNDIGTSLGG
                     EGRDTSAADAVVAEIIAAGGRAIANYEDITSWEAAGRIVEATLAAFGDLHVVVNNAGI
                     VRDRMFVSATPEEWDATMHVHLRGHFCLARHAVNYWRGQQKAGKAVDARLINTSSGAG
                     LQGSVGQSAYATAKGGIAALTLVQAAELGRYGITANALAPSARTRMTEGAFADKMATR
                     GDAFDVMDPANIAPAVVWLGSAQSAHVTGCVFELEGGRIMLEDGWREGPCADRGARWA
                     PGEVGAAVDRLLAERVPPRKVWGA"
     gene            complement(1500..1970)
                     /locus_tag="VFF98_07555"
     CDS             complement(1500..1970)
                     /locus_tag="VFF98_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63522.1"
                     /translation="MAGVDTTLPAGVQITNLLALYAEHFDAGDFEATAALFDRGCVIV
                     GSKVLTGRAAIMAMWRSVVRVYPGGRLHTRHLMTNPIITPGATADELACRSQWTVLQA
                     APGLALQVIATGRYHDIFRRAGSAWHFASRTYAGIDLAGDTTAHLLHPIAREEA"
     gene            complement(1970..3121)
                     /locus_tag="VFF98_07560"
     CDS             complement(1970..3121)
                     /locus_tag="VFF98_07560"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZU63523.1"
                     /translation="MPEAYIIDAVRSPVGRKKGRLAHLHPADLAAHPLRVLMARAGFD
                     PVLVEDVVWGCCDTIGPQAGDIGRTAWLAAGLPEEVPGVTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVVAGGSQAMNAIPISAAMFAGEAYGFASPFAGARGWDARYGGEEVNQIRS
                     AEMIAGKWGLTRAAMEQFALASHQRAQAATEAGWFAKEIAPLEGLAHDETIRPATTLE
                     GLAALKPVREGGLVTAGIASQNGDAAAALLIASARAVKEHKLTPRARIHHLSVRAADP
                     VWMLTGPIPATRHALQKAGMRIEDIDLFECNEAFASVTMAWMQELAIPHEKVNVAGGA
                     IALGHPIGATGARLMTSLLHALERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            complement(3114..4151)
                     /locus_tag="VFF98_07565"
     CDS             complement(3114..4151)
                     /locus_tag="VFF98_07565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63524.1"
                     /translation="MDFTLSDEQKMFAETARSLLVDRCTPEDWRQLLASGAARDAARW
                     AAIVENGLTLLLLPEAAGGLGLGELDFALIAEQAGYVALPEPLVESAGIAAPMLAARA
                     PDAAVLADAGATIAIQHPANPLVADADSAAAVLLHHEGEAWLAPAAGVQLTRVASIDP
                     FRRLFRVEWQREAAERLGPADWDLALERGALFAAAQGLGLAQRCVDMAVAYAQERQQF
                     GKPIGSYQAVKHHLASAQVAIAFARPVVHAAAAGLAARDIHARARVSHARIVALEAAD
                     QAARAALQVHGAMGYSWEVDVHLFLKRALALNGAWGTPRFHRDRIAARLFTRPNGPDQ
                     TFARESETSHA"
     gene            complement(4165..5310)
                     /locus_tag="VFF98_07570"
     CDS             complement(4165..5310)
                     /locus_tag="VFF98_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU63525.1"
                     /translation="MRLAYTPQQQAFRAEVRSWLAAHVPATPLDHFDASRAGFEAHRQ
                     WEKTLKSGDWGMVTWPREYGGRGLNLIEWLIFEEEYYRAGAPGRVNQNGIFLLGPTLM
                     EFGTAAQKQRFLPAMASGEEIWAQAWSEPQAGSDLAGVRATCRRDGEDYVLNGHKIWS
                     SRAVFADWAFGLFRTPGSERHKGMSLIFFPLNAPGVTVNPIAKINGHVGFAEIFLEEV
                     RVPAFNRLGEEGQGWHICMATAGFERGLMLRSPARYQVAAAALADLWLARRHIADPLL
                     EADVVRAHMNAEAYALSIYATASALMAGAKIGPEASTNKIFWSELDIHLHRTALAILG
                     AEAELLPPPGTHDWLDDYIFALAGPIYAGSNEIQRNIIAERMLGLPR"
     gene            complement(5310..6065)
                     /locus_tag="VFF98_07575"
     CDS             complement(5310..6065)
                     /locus_tag="VFF98_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZU63526.1"
                     /translation="MPIDLTIAERIAEIVFDVPPVNAFDSATWNTLPALIRDAAARPD
                     VHCVLIRAAEHARGFCGGVDIKEMQAHPERITLLNRGNYLTFQAVRAAEVPVVVAVHG
                     FVIGGGIGICGAADTIIAAEDAYFSLPEVDRGAMGGASHLSRMLPLHKVRAAFFTGGT
                     IAAAEAWRLGGVEKVVPRADLLAEARAFCGQIAAKSRKALVIAKEALNGLEPRDVDRG
                     YRWEQGFTLEMYMHEDSQKARDAFVATKGAARF"
     gene            complement(6055..7170)
                     /locus_tag="VFF98_07580"
     CDS             complement(6055..7170)
                     /locus_tag="VFF98_07580"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZU63527.1"
                     /translation="MGDPLHTILCERLGCTAPIIQTAMGWVAEPSLVIASSNAGAFGF
                     LGAAVMTADEARAKILAVRRATDRPFGVNFHAFQPGAEQIVELILENRAQVRAVSFGR
                     GPNARMIGRFRDAGILCIPTVGAVKHAVKMAELGVDMISVQGGEGGGHTGAVPTTVLL
                     PQVLDAVGVPVIASGGFADGRGLAAALAYGAVGIAMGTRFLMTRESPVPAAAKAAYLK
                     AGTEGIVLTTKLDGIPQRIIRTPLVDRIERSGRLGMWLRAIEASAAMKRQTGASWLEL
                     IRAARGMTAHGQMPLMQAMMAATMPMLIQKAVVAGDVDQGVLATGVVGGRIDDMPGCA
                     ELVARICAEARARLCALVADTPATPGQHSIAGEFHAD"
     gene            complement(7170..8084)
                     /locus_tag="VFF98_07585"
     CDS             complement(7170..8084)
                     /locus_tag="VFF98_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZU63528.1"
                     /translation="MSDLVTPRQADITYETDSPVLYAVEGRVAWITMNRPAFNNAQNG
                     QMTYALDDAFQRAVADDGVGAIVLAGAGRHFSAGHDIGTPGRDLHHEFDKRLMLPGHT
                     NKPAAELLYTREQEQYLGMCRRWRDIPKPTIAMVQGACVAGGLMLAWVCDLIVASEDA
                     FFQDPVNPLMGIPGVEYFAHAYELPPRVAKEFLLLGERMEAARAARFGMVNKVVPRAE
                     LQAATAAWADKLAAQPRLGNWLTKQAVNHVEELRGKRAAMDAVFHMHHFAHAQNDLVT
                     GNAIAGVSGQSAAAANKQAAGKQAAGEG"
     gene            complement(8081..8884)
                     /locus_tag="VFF98_07590"
     CDS             complement(8081..8884)
                     /locus_tag="VFF98_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HZU63529.1"
                     /translation="MSEHAHDITLAELCIFACSEAFRGNGEVVATGVGPVPRLAAGLA
                     KLTHSPELMMTDGEAYLVEQPVPIGPRAYDDRKPAGHLPFSRFFDAAVWSGRRHAMVT
                     PTQIDRFGQINLSFMGGTYRQPKTQMLGVRGFPGNTICHPNSFFFPAHSPRVFVAGEV
                     DMISGVGYNPAKRVAGGHFAGVDLRRIVTNLAVMDFGGADHALRVVSLHPGVSFEAVQ
                     AATGFALENAVAGETALPTAEALAIIARLDPHNIRASVIKDNPPARRAA"
     gene            complement(8884..9762)
                     /locus_tag="VFF98_07595"
     CDS             complement(8884..9762)
                     /locus_tag="VFF98_07595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZU63530.1"
                     /translation="MLDKRMSAADIVAQLHDGMTIGIGGWGPRRKPMALVREILRSDL
                     KDLTVVAYGGADVGMLCAAGKVRKLVFAFVSLDAIALEPWFRKAREAGTLDVLELDEG
                     MFQWGLKAAAFGLPFLPTRVGLGTDLAELGGLKLVHSPYADGEALIAMPALKLDAALL
                     HVNRSDWRGNVHIFGPDVYYDEWFARAAAKTYVSCEELVDRMEDHYPAEAHHNIVERS
                     FISGVVELPGGAHPSSMPPHYGWDMKAFKAYADAARDPGDWAAVAGRFVGPSEALYLE
                     HLGGKEAVAALALPIF"
     gene            complement(9771..>10460)
                     /locus_tag="VFF98_07600"
     CDS             complement(9771..>10460)
                     /locus_tag="VFF98_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU63531.1"
                     /translation="VADGSPEEAAARGVTAFFRTSDPAGNGLEFFHGDQRDAVPFVSP
                     AGVSGFVTGEMGMGHAVFATPDFAAAHDFYKEVVGFGDTDLPRFRFSDDPADPGMGFA
                     FMHADNGRHHSIAFAEMPVPPSRCVHLMLEMATMADVGRCHDRMRAHKVPESATLGRH
                     VNDRTFGFYMQTPSGFDLEIGWDPLVIDPASWACTAHLQPSEWGHEWAWQKAMAEAQG
                     EAQAKTQEGGQ"
BASE COUNT         1674 a         3774 c         3441 g         1571 t
ORIGIN      
        1 ggccagctcc tgcgcgcgcg tgccggccag cgccagcgtc gccagcgggc gggtctggtc
       61 catcgtcaca tgcgccgaaa cgctgagccc tgcggcattg cgctccacca gccaggcgcc
      121 cttgccggcg gccaccagaa acagatcggc cagcgcccca tgcgccacga aatcagccac
      181 cagcgagagc gccccgccag cctcggtcac ggtttcggcc tgcgcgaagg ctgcgatcgc
      241 cgcgcccgac agcagctgcg gcaaccattg cgcctgctgc tgggcgctgc cgccggcggc
      301 cagcgcgcgg gccaccatgg catggcccag cagcggcacc gcggccacct gcccgccagc
      361 cgcctgcgcc acgatcgcca gttccacaag gcccaggccc gcgccgccca gcgcctcggg
      421 cacggccacc ccggccatgc ccagctctgt gcagaaagcg ccccacaggg cccggtcgat
      481 cccctcgccg gccatcgccg cgcgggtgcg ttcgctggtg gcgtgttcgg caaagaagcc
      541 gttcacggtt tcagcgatca tctcctgttc ttcggtgaac gcgaattcca tcgccttatg
      601 ctccccacac cttgcgcggc ggcacacgtt ccgccagcag cctgtccacc gccgcgccca
      661 cttcgccggg cgcccagcgc gcgccccggt cggcgcaggg cccctcgcgc cagccatcct
      721 ccagcatgat ccggccgcct tccagctcga acacgcagcc cgtcacatgg gccgattgcg
      781 cagagcccag ccacaccacc gccggggcga tattggccgg gtccatcacg tcgaaggcat
      841 cgccccgggt ggccatcttg tcggcaaagg cgccctcggt catgcgggtg cgggccgagg
      901 gggcaagggc attggcggtg atgccatagc ggcccagctc ggccgcctgc accagcgtca
      961 gcgcggcaat gccccccttg gcggtggcat aggccgattg cccgacagag ccctgcagcc
     1021 ccgccccgct cgacgtgttg atcagccgcg catcgaccgc cttgccggcc ttctgctgcc
     1081 cccgccaata attcaccgca tggcgcgcca ggcagaaatg gccgcgcaga tggacatgca
     1141 tcgtcgcatc ccattcctcc ggcgttgccg agacgaacat cctgtcgcgc acgatgccgg
     1201 cattgttgac caccacatgc agatcgccaa aagcggcaag cgtcgcctcg acaatgcgcc
     1261 cggccgcctc ccagctggtg atgtcctcgt aattggcgat ggcgcggccg ccggcggcga
     1321 tgatctcggc caccaccgca tcggcggcgc tggtatcacg cccctcgccg cccagcgagg
     1381 tgccgatgtc gttcaccacg acacaggccc cctcggccgc gaaagccagc gcataggcgc
     1441 ggcccaggcc gccggcagcc ccggtgatga taacggtgcg attgtcgcag atgcccatgt
     1501 caggcctcct ctcgggcaat gggatgaagc aggtgggccg tggtgtcgcc cgccagatcg
     1561 atgccggcat aggtgcggct ggcaaaatgc caggcgctgc ccgcgcggcg gaagatgtcg
     1621 tgatagcggc cggtggcgat cacctgcagg gcaaggccgg gggcggcctg cagcaccgtc
     1681 cattgcgacc ggcaggccag ctcgtcggca gtggcgccgg gcgtgatgat cgggttggtc
     1741 atcaggtggc gggtgtgcag ccgcccgccg ggatagaccc gcaccaccga ccgccacatc
     1801 gccatgatcg ccgcgcgccc tgtcagcacc ttgctgccga cgatcacgca gccccggtcg
     1861 aacagggccg cggtcgcctc gaaatcgccg gcgtcgaaat gctcggcata aagggccagc
     1921 agattggtga tctgcacccc ggccggcagg gttgtgtcaa cgcccgccat cacagccgct
     1981 cgatgatggt gacattggcc tggccgccgc cttcgcacat cgtctgcagg ccatagcgcc
     2041 cgccggtgcg ttccagcgcg tggagcaggc tggtcatcag ccgcgcgccg gtggcgccaa
     2101 tgggatggcc cagcgcaatc gcgccgccgg ccacgttcac cttctcgtgg gggatggcca
     2161 gttcctgcat ccaggccatc gtcacgctgg cgaaagcctc gttgcattcg aacaggtcga
     2221 tgtcctcgat ccgcatgccc gctttctgca gtgcatggcg cgtggccggg atcgggccgg
     2281 tgagcatcca caccggatcg gccgcccgca ccgagaggtg gtggatgcgc gcgcgcggcg
     2341 tgagcttgtg ttccttcacc gcccgcgcgc tggcgatcag cagagccgcg gcggcatcgc
     2401 cgttctggct ggcgatgccg gcggtaacca gcccgccctc gcgcaccggc ttcagcgcgg
     2461 ccaggccctc cagcgtggtt gccgggcgga tcgtctcgtc atgggccagg ccctccagcg
     2521 gggcgatctc tttggcaaac cagccggcct cggtggcggc ctgggcgcgc tggtggctgg
     2581 ccagggcaaa ctgctccatc gccgcgcggg tgaggcccca tttcccggcg atcatctcgg
     2641 ccgagcggat ctggttgacc tcctcgccgc catagcgcgc atcccagccg cgcgcgccgg
     2701 caaaggggct ggcaaagcca taggcctcgc cggcgaacat ggccgccgag atcgggatgg
     2761 cgttcatcgc ctggctgccg ccggccacca ccagatcctg ggtgccgctc atcaccccct
     2821 gggcggcaaa atgcaccgcc tgctgcgaag agccgcattg ccggtcgatg gtaacgccgg
     2881 gcacttcctc gggaaggccg gccgccagcc aggcggtgcg gccgatatcg cccgcctgcg
     2941 ggccgatcgt gtcgcagcag ccccacacca catcctcgac cagcaccgga tcgaagccgg
     3001 cgcgggccat cagcacgcgc aaggggtggg cggcaagatc ggccgggtga agatgcgcaa
     3061 ggcgcccttt tttgcgaccc acagggctgc gcacggcatc gatgatatag gcttcaggca
     3121 tggctcgtct cgctctcgcg ggcaaaggtc tggtcggggc cgttgggccg ggtgaagagg
     3181 cgcgcggcga tgcgatcgcg gtggaagcgc ggcgtgcccc aggcgccgtt cagcgccagc
     3241 gcccgcttga ggaagagatg cacatccact tcccagctgt agcccatggc gccatgcacc
     3301 tgcagcgcgg cgcgggcggc ctgatccgcc gcttccagcg ccacgatccg ggcatggctg
     3361 acacgggcgc gggcatggat atcgcgcgcg gcaaggccgg cggcggcggc atgcaccacc
     3421 ggccgggcaa aggcgatggc cacctgggcg ctggccagat gatgcttcac cgcctgatac
     3481 gagccgatcg gcttgccgaa ctgctggcgc tcctgcgcat aggccacggc catgtccacg
     3541 cagcgctggg ccaggcccag cccctgcgca gcggcaaaca gcgcgccgcg ctccagcgcc
     3601 agatcccagt cggcagggcc caggcgctcg gccgcctcgc gctgccattc cacgcggaac
     3661 aggcggcgga agggatcgat gctggccaca cgcgtcagct gcacgccggc ggccggggcc
     3721 agccaggcct cgccctcgtg gtgcagcagc acagctgccg cgctgtcggc atcggccacc
     3781 agcgggttgg ccggatgctg gatggcaatg gtggcgccgg catcggccag caccgccgca
     3841 tcgggcgccc gcgcggccag catgggcgcg gcaatgccgg cgctttccac cagcggctcg
     3901 ggcagggcga catagccggc ctgctcggcg atcagcgcga aatcaagctc gcccaggccc
     3961 agcccgccgg ccgcctcggg cagcagcagc aaggtcaggc cattctcgac aatcgccgcc
     4021 cagcgcgccg catcgcgcgc cgcgccgctg gccagcagct ggcgccagtc ctccggcgtg
     4081 cagcggtcga ccagcaggct gcgggcggtc tcggcaaaca tcttctgttc atcggagagg
     4141 gtgaaatcca tcgccagcct cctctcagcg cggcaggccg agcatgcgct cggcaatgat
     4201 gttgcgctgg atctcgttcg atccggcata gatcggcccg gccagcgcga agatgtaatc
     4261 gtccagccag tcatgcgtgc cggggggtgg cagcaattcg gcctcggccc ccaggatcgc
     4321 cagcgccgtg cggtggaggt ggatgtccag ctccgaccag aagatcttgt tggtgctggc
     4381 ctcgggcccg atcttcgcgc cggccatcag cgcgctggcg gtggcataga tgctcagcgc
     4441 ataggcctcg gcgttcatgt gggcgcgcac cacatcggct tccagcagcg gatcggcgat
     4501 gtgccggcgc gccagccaca gatcggcaag ggcggccgcc gccacctgat agcgcgccgg
     4561 gctgcgcagc atgaggccgc gctcgaaccc ggccgttgcc atgcagatgt gccagccctg
     4621 cccttcctcg cccagccggt tgaaggcggg cacgcgcacc tcctccagaa agatctcggc
     4681 aaagccgaca tggccgttga tcttggcgat cgggttcacc gtcacgcccg gcgcgttcag
     4741 cgggaagaag atcaggctca tgcccttgtg gcgctcgctg cccggcgtgc ggaacaggcc
     4801 aaaggcccaa tcggcaaaca ccgcccggct ggaccagatc ttgtgcccgt tgagcacata
     4861 atcctcgcca tcgcgccggc aggtggcgcg cacgccggcc agatcgctgc ccgcctgcgg
     4921 ttccgaccag gcctgcgccc agatctcctc gcccgaggcc atcgccggca ggaagcgctg
     4981 cttttgcgct gcggtgccaa attccatcag cgtggggccc agcaggaaga tgccgttctg
     5041 gttgacccgg ccgggcgcgc cggcgcggta atattcctcc tcgaagatca gccattcgat
     5101 gaggttcagc ccgcgcccgc catattcccg gggccaggtc accatgcccc agtcgcccga
     5161 tttcagggtc ttttcccatt ggcgatgggc ctcgaaaccg gcgcgcgagg catcgaaatg
     5221 gtccagcggc gtggccggca catgtgctgc cagccagctg cgcacctcgg cgcggaaggc
     5281 ctgctgttgg ggggtatagg ccagccgcat cagaaccgcg ccgccccctt ggtggccacg
     5341 aaggcatcgc gcgccttctg gctgtcttca tgcatgtaca tttccagcgt gaagccctgc
     5401 tcccagcgat agccgcggtc gacatcgcgc ggctccagcc cgttcagcgc ttccttggcg
     5461 atcaccagcg ccttgcggct cttggcggcg atctgcccgc aaaaggcgcg cgcctcggcc
     5521 agaagatcgg cgcgcggcac caccttttcc accccgccca gccgccaggc ctcggccgcc
     5581 gcaatcgtgc cgccggtgaa gaaggccgcc cgcaccttgt gcagcggcaa catgcgcgac
     5641 aggtggctgg ccccgcccat cgccccccga tccacttcgg gcagcgagaa ataggcatcc
     5701 tcggcggcga tgatcgtgtc ggccgcgccg cagatgccaa tgccgccgcc gatgacaaag
     5761 ccgtgcaccg ccaccaccac cggcacctcg gccgcgcgca ccgcctggaa ggtgagataa
     5821 ttgccgcggt tgagcagggt gatccgctcg gggtgggcct gcatctcctt gatgtcgaca
     5881 ccgccgcaaa agccgcgggc gtgctcggcc gcgcggatca gcacgcaatg cacatcgggc
     5941 cgggccgctg catcgcggat cagcgcgggc agcgtgttcc aggtcgcgct gtcgaaggcg
     6001 ttcaccggcg gcacgtcgaa gacgatctcg gcgatgcgct cggcaatggt caggtcaatc
     6061 ggcatgaaac tctcccgcga tgctgtgctg gccgggcgtt gccggcgtgt ctgcgaccag
     6121 ggcgcaaagg cgcgcgcgcg cctcggcgca gatgcgcgcc accagctctg cgcagcccgg
     6181 catgtcgtct atccgcccgc cgaccacgcc ggtggccagc accccctgat cgacatcacc
     6241 cgccaccacc gccttctgga tcagcatcgg catggtggcg gccatcatcg cctgcatcag
     6301 cggcatctgg ccatgggcgg tcatgccgcg ggcggcgcgg atcagctcca gccagctggc
     6361 gcctgtctgc cgcttcatcg ccgcgcttgc ctcgatggcg cgcagccaca tgcccagccg
     6421 cccggaacgc tcgatgcgat cgaccagcgg cgtgcggatg atccgctggg gaatgccatc
     6481 gagcttcgtc gtcaacacga tgccctcggt gccggccttc agataggcgg ccttggcggc
     6541 agcgggcacc gggctttccc gcgtcatcaa aaagcgcgtg cccatggcaa tgcccaccgc
     6601 gccataggcc agcgccgcgg caaggccgcg cccatcggca aagccgccgc tagcgatcac
     6661 cggcacgccc accgcatcga gcacctgcgg caacagcacc gtggtgggca cagcgccggt
     6721 gtgcccgccg ccctcgccgc cctgcacgct gatcatgtcg acgcccagtt cggccatctt
     6781 caccgcatgt ttcaccgccc ccacggtggg gatgcacagg atgccggcat cgcggaagcg
     6841 gccgatcatc ctggcattgg gcccgcgccc gaagctgacc gcgcgtacct gcgcgcggtt
     6901 ctcgaggatc agctcgacga tctgctcggc gcccggctgg aaggcgtgga agttgacgcc
     6961 gaagggccgg tcggtggcgc ggcgcacggc caggatcttg gcgcgcgcct catcggccgt
     7021 catcaccgcc gcgccaagga agccgaaagc gccggcattg ctactggcga tcaccagcga
     7081 aggctcggcc acccagccca tcgccgtctg gatgatcggc gcggtgcagc ccagccgctc
     7141 gcacaggatg gtgtgcaggg gatcgcccat cagccctctc ctgccgcctg tttgcccgcc
     7201 gcctgcttat tcgccgccgc cgccgactgg ccgctgacgc cggcaatcgc attgccggtc
     7261 accagatcgt tctgggcatg ggcaaagtgg tgcatgtgaa acaccgcatc catcgccgca
     7321 cgcttgccgc gcagctcttc cacatggttc acggcctgct tggtcagcca gttgcccagc
     7381 cggggctggg cggccagctt gtctgcccag gcagcggttg ccgcctgcaa ttcggcccgg
     7441 ggcaccacct tgttcaccat gccgaaccgg gccgcgcgcg ctgcctccat ccgctcgccc
     7501 agcagcagga attccttggc cacgcgcggc ggcagttcgt aggcatgggc gaaatattcc
     7561 acgcccggaa tgcccatcag cggattgacc ggatcctgga agaaggcatc ctcgctggcc
     7621 acgatcagat cgcacaccca ggccagcatc agcccgccag ccacgcaggc cccctgcacc
     7681 atggcgatgg tgggcttggg aatatcgcgc cagcgccggc acatgcccag atattgctcc
     7741 tgctcgcggg tgtagagcag ttcggcggcc ggcttgttgg tgtggccggg cagcatcagc
     7801 cgcttgtcga actcgtgatg caggtcgcgc ccgggcgtgc cgatgtcatg gccggccgag
     7861 aaatgcctgc ccgccccggc cagcacgatg gcgcccacgc catcatcggc caccgcgcgc
     7921 tggaaggcat cgtcgagcgc ataggtcatc tgcccgttct gggcattgtt gaacgcgggc
     7981 cggttcatgg tgatccaggc cacccgccct tccaccgcat agagcaccgg gctgtccgtc
     8041 tcgtaggtga tatccgcctg gcgcggggtg acgagatcgc tcatgccgcc ctccgtgccg
     8101 gggggttgtc cttgatgacg ctggcgcgga tattgtgcgg atcgagccgg gcgatgatcg
     8161 ccagcgcctc ggcggtgggc agcgcagtct cccctgccac cgcattctcc agcgcaaagc
     8221 cggtggccgc ctgcaccgcc tcgaagctga cacccggatg cagcgagacg acacgcagcg
     8281 catgatcggc cccgccaaaa tccatcaccg ccagattggt gacgatgcgc cgcagatcga
     8341 caccggcgaa atggccgccc gccacccgct tggccggatt ataccccacg cccgagatca
     8401 tatcgacctc gcccgccacg aacacgcgcg gcgaatgggc ggggaagaag aaggaattgg
     8461 ggtggcagat ggtgttgccg ggaaagccgc gcacgcccag catctgcgtc ttgggctggc
     8521 gataggtgcc gcccatgaag gagaggttga tctggccgaa gcggtcgatc tgggtggggg
     8581 tgaccatggc atggcgccgg ccgctccaca ccgcggcatc gaaaaagcgc gagaagggca
     8641 ggtggccggc cggcttcctg tcgtcatagg cgcgcgggcc gatcggcacc ggctgctcca
     8701 ccagataggc ctcgccatcg gtcatcatca gctcggggct gtgggtcagc ttggcaaggc
     8761 cggcggccag gcgcggcacc gggccaacgc cggtggccac cacctcgcca ttgccgcgga
     8821 aggcctcgga acaggcaaag atgcacagtt cggccagggt gatatcgtgt gcgtgctcgc
     8881 tcatcagaaa atcggcagcg caagagcggc caccgcctcc tttccgccaa gatgttccag
     8941 ataaagggct tcggaagggc ccacgaagcg cccggccacc gccgcccagt ctcccggatc
     9001 gcgcgcggca tcggcatagg ccttgaaggc cttcatatcc cagccataat gcggcggcat
     9061 cgagctgggg tgggcgccac cgggcaattc caccacgccg ctgatgaagg accgctcgac
     9121 gatattgtgg tgggcctcgg ccgggtaatg gtcctccatc cggtcgacca gttcctcgca
     9181 gctgacatag gtcttcgcgg cggcccgggc gaaccattcg tcgtaataga catcgggccc
     9241 gaagatatgg acattgccgc gccagtcgct gcggttcaca tgcagcagcg ccgcatccag
     9301 cttcagcgcc ggcatggcaa tcagcgcctc gccatcggca taggggctgt gcaccagctt
     9361 cagcccgccc agctcggcca gatccgtgcc cagccccacg cgggtgggca ggaagggcag
     9421 gccaaaggcg gccgccttga gcccccactg gaacatgccc tcgtccagct ccagcacgtc
     9481 gagcgtgccg gcctcgcgcg ccttgcggaa ccacggctcc agcgcgatcg catcgaggct
     9541 gacaaaggcg aagaccagct tgcgcacctt gcccgccgcg cacagcatgc ccacatccgc
     9601 gccgccatag gccaccacgg tgagatcctt cagatcggag cgcaggatct cgcgcaccag
     9661 cgccatcggc ttgcgccgcg ggccccagcc gccgatgccg atggtcatcc cgtcgtgcag
     9721 ctgcgcgacg atgtcggccg cgctcatccg cttgtccagc atcgccggca tcactggccc
     9781 ccttcctgcg ttttggcctg cgcttcgccc tgcgcttcgg ccatggcctt ttgccaggcc
     9841 cattcatggc cccactcgct gggttgcaga tgggcggtgc aggcccagct ggccggatcg
     9901 atcaccagcg gatcccagcc gatctccagg tcgaagcccg aaggcgtctg catgtagaag
     9961 ccgaaagtgc ggtcgttcac atggcggccc agcgtggcgc tttcgggcac cttgtgggcg
    10021 cgcatgcgat catggcagcg gccgacatcg gccatcgtcg ccatttccag catcagatgc
    10081 acgcagcgcg agggcggcac cggcatttcg gcaaaggcga tcgaatggtg gcggccattg
    10141 tcggcatgca tgaaggcaaa gcccatgccg ggatcggccg gatcatcgct gaagcggaag
    10201 cgcggcaggt cggtatcgcc aaagcccacc acctccttgt agaaatcatg ggccgcggca
    10261 aaatcgggcg tggcgaacac cgcatggccc atgcccatct cgcccgtcac aaagccggag
    10321 acgccggcgg gcgaaacgaa gggcaccgcg tcgcgctggt cgccatggaa gaattccagc
    10381 ccgttgccgg ccggatcgct ggtgcggaaa aaggccgtaa caccgcgcgc ggcggcttct
    10441 tcggggctgc catcggccac
//
LOCUS       DASRYE010000140         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_218798,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000140 DASRYE010000000
VERSION     DASRYE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218798"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1665)
                     /locus_tag="VFF98_07605"
     CDS             complement(367..1665)
                     /locus_tag="VFF98_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZU63532.1"
                     /translation="MLSDLTEQGAMAQEGAAWPAPPRRPQSLPPRHLWREWMAIIWPA
                     VGVSVMLTFPALGVAGPILFLLLVAVQWVPISAVPMQRVIRCWPYALLFLFAMLSTLW
                     STAPLATLRYSAQLAITMIAGLMMGACAWPRRVMLSYAVGLVATVALGLVFGQSAAWG
                     AQGDTAFVGLGGGKNYFADWCATALVMWVGAMAAMGPGGRGWRLALSLPVVAVLLAGL
                     ISTHSTGSTAALAISCLLMAAMGVMRLLRPPVRAAMLALALALVGVGSVAMSQPEVRS
                     EILHMLNKDENLTGRRYLWYRGQGYMDERPWLGTGYGAFWLQGNLDAEGLWYINGITG
                     RKGFNFHNIAIETRVNMGLVGFGLLVALCLGAVLSILAGFLRRPLATDLGWLGFALYE
                     MSRVTFETVGPTPFTHDTLLFCSVMGYAAVRRLNLAAQER"
     gene            complement(1655..3034)
                     /locus_tag="VFF98_07610"
     CDS             complement(1655..3034)
                     /locus_tag="VFF98_07610"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU63533.1"
                     /translation="MRVTGANRASGRGRPGSGRPGKGWALGAVAIAAGAQAGPCLAQS
                     AILPPVAPALSPGGYAPPFTPVPEGVSVADRARPDYQPVGGRLGAFFLYPQLSANWGI
                     TDNVRAVDEQRIGDNYWDLQGGAQLVSGFSRHQLKLGGYAGRSFYQGHAGEDGTRYGG
                     SLAARLDFAGQTSIDLTAAAEHLLLARDDYNSPYGAASPVRYDQLQVKLGASRVFNRL
                     TLRALGGVLGLTYHSPLSDTGALIDQRYRSRTGYSVLMRADYELQPGVSLVARAVWDD
                     QAYRLPASDPAQPGGLARSSVGWRAEGGLAFRLTGKLYGEARLGYMRRRYDDARMLTA
                     KGLSFGADLAWNLDARTSLRLTADRRVDETASITSAGARVSEFDLRVEHELARNLILT
                     AEGDYQRQNPLGPDTPSTEWRGEANARWLINRRLSAKVTYRHAQRACTDPAHAFRSNT
                     GLVGVTLAF"
BASE COUNT          550 a         1222 c         1032 g          460 t
ORIGIN      
        1 caaattccac cgccgctgcc gttagcgcca gaaccccgcc ggcaacgccg caaaacagcc
       61 acatccgccg gcggaacacc gcccacacct cggccggatc tggcaacagc cgctgcatga
      121 cggcctcggc cccgggcata aagccgttca atccctgtga tgccatagcg ctttcaccct
      181 tgcctgatcc atctggcaca gaagcttttg cttcggccct gggcagcacc cgtgccgggc
      241 gcttgtgtcg ttcgatctat gcgccgccta agggattgac ccaaggaata gcccgtccat
      301 ttcagaacag ttcgtcgcca gctgcgtaca aaaccgaggc attcccgcag cgcgcgaggc
      361 ccgccttcag cgctcctgcg ccgccaggtt cagccggcgc accgccgcat agcccatcac
      421 gctgcagaac agcagcgtgt catgcgtgaa aggggttggc cccactgtct cgaaggtcac
      481 gcgggacatt tcatacagcg caaagcccag ccacccgaga tccgtggcca gcggccggcg
      541 caaaaagccc gccaggatcg acagcaccgc ccccaaacac agcgccacca gcaggccaaa
      601 gccgacaagg cccatgttca cccgcgtttc gatggcgatg ttatgaaaat tgaagccttt
      661 gcggccggtg atcccgttga tgtaccacag cccttcggca tcgagattgc cctgcagcca
      721 gaaggcgcca tagcccgtgc ccagccacgg ccgctcgtcc atatagcctt gcccgcgata
      781 ccacagatag cgccggccgg tgaggttctc gtccttgttc agcatgtgca ggatctcgga
      841 acgcacctcg ggctggctca tcgccacgct gcccacgccc accagtgcca gcgccagcgc
      901 cagcatcgcc gcccgcaccg gggggcgcag cagccgcatc acccccatgg ccgccatcag
      961 caggcaggag atcgccagcg ccgccgtcga tccggtggaa tgggtgctga tcagccccgc
     1021 cagcagcacc gccaccaccg gcaggctgag cgccaggcgc caaccgcgcc cgcccggccc
     1081 catggccgcc atggccccca cccacatcac cagcgccgtc gcgcaccagt cggcgaaata
     1141 gttcttgccg ccgcccagcc ccacaaaggc ggtgtcgccc tgcgcgcccc aggcggcaga
     1201 ctggccgaac accagcccca gcgccaccgt tgccaccagc cccaccgcgt aagacagcat
     1261 cacccgccgc ggccaggcgc aggcgcccat catcagcccg gcgatcatgg tgatcgccag
     1321 ctgcgcgcta tagcgcaagg tggcgagcgg cgccgtgctc cacaaggtgg acagcatggc
     1381 gaacaggaac agcagcgcat agggccagca gcgtatcacc cgctgcatcg gcaccgcgct
     1441 gatcggcacc cattgcaccg ccaccagcag caggaacagg atgggccccg ccactcccag
     1501 cgcgggaaag gtgagcatca cgctcacccc cacggcgggc cagatgatcg ccatccattc
     1561 gcgccagagg tggcgcggcg gcaggctttg cgggcggcgc ggcggcgcgg gccaggcggc
     1621 cccctcttgc gccatggcgc cctgttccgt caggtcagaa agcaagggtc acccccacaa
     1681 ggccggtgtt ggaacggaag gcatgggccg gatcggtgca ggcgcgctgg gcatggcgat
     1741 aggtcacctt cgcgctcagc cggcggttga tcagccagcg ggcattggcc tcgccccgcc
     1801 attcggtgga gggcgtatcc ggccccagcg ggttctggcg ctgatagtcg ccctcggccg
     1861 tcaggatcag gttgcgggcc agctcatgct ccacccgcag gtcgaactcg ctgacgcgcg
     1921 cgcccgcgct ggtgatcgag gcggtctcat cgacccgccg gtcagccgtg agcctgaggc
     1981 tggtgcgcgc atcgaggttc cacgccagat cggcgccgaa tgacaggccc ttggccgtca
     2041 gcatccgggc atcgtcgtag cggcggcgca tatagcccag ccgcgcctcg ccataaagct
     2101 tgccggtgag gcgaaaggcc aggccccctt ccgcccgcca gcccaccgaa ctgcgcgcca
     2161 gcccgcccgg ctgggccgga tcgctggcgg gcaggcgata ggcctgatca tcccacaccg
     2221 cgcgggccac caggctgacg ccgggttgca gctcgtaatc ggcccgcatc agcacgctat
     2281 agccggtgcg gctgcgatag cgctggtcga tcagcgcgcc ggtatcgctg agcgggctgt
     2341 ggtaggtgag cccgagaacg ccgcccagcg cccgcagcgt caaccggttg aacacgcgcg
     2401 acgcgcccag cttcacctgc agctggtcgt agcgcaccgg gctcgccgcg ccataggggc
     2461 tgttgtaatc atcgcgggcc agcagcaggt gctcggccgc ggcggtcagg tcgatgctgg
     2521 tctgcccggc aaagtcgagc cgcgcggcca gcgatccgcc atagcgcgtg ccatcctcgc
     2581 ccgcatgccc ctgatagaaa ctgcgcccgg catagccgcc cagcttcaac tggtggcgcg
     2641 aaaagcccga aaccagctgc gcgccgccct gcagatccca gtaattgtcg ccaatgcgct
     2701 gctcgtccac cgcgcgcaca ttgtcggtga tgccccagtt ggcggacagc tgcggataca
     2761 ggaagaaggc gcccagccgg cctcccaccg gctgataatc cggccgggcc cgatccgcca
     2821 cgctcacccc ttcgggcacc ggggtgaagg gcggggcata gccgcccggc gacagcgccg
     2881 gcgccaccgg cggcagaatg gcgctttgcg caaggcaggg cccggcctgc gccccggcgg
     2941 caatggccac cgcgcccagt gcccagccct tgcccggcct gccgctgccc ggcctgccgc
     3001 gcccgcttgc cctgttcgcg cctgtcaccc tcatcaactg tcgccctcgt cggttccgtc
     3061 tcgtccgttc cgtccgcgcg gatggccggt gaacgggcca tcctgctgcc gctgccggcg
     3121 cgatgtgccg ccgggtgtgg cccaggatca gctaggtgaa aacgcgttgt cgcaccgttt
     3181 tggcaccaat tgcccggtcg gatcggcatc ggcaggggct gttgccggcc ccgccggggg
     3241 caatcggggt gtggccggtg cgcg
//
LOCUS       DASRYE010000141         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_23474,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000141 DASRYE010000000
VERSION     DASRYE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23474"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1125
                     /locus_tag="VFF98_07615"
     CDS             25..1125
                     /locus_tag="VFF98_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZU63534.1"
                     /translation="MADAPKPAAPSASGRASTKKELPEHFRAYKHVWVAVEIEHGAVH
                     PVSFELLGEGRKLADKRGVELAAVVMGDARADLDVAATHCFEYGADVVYIAQDPALAH
                     YRNETACDVLTHLVNTYKPEILLLGATNLGRDLAGAVATTLQTGLTADCTELAIDEEG
                     SLAATRPTFGGSLLCTIFTLNYRPQMATVRPRVLPMPPRQEGRGGRIITYSPAIDETR
                     VITKVLDFIADRDSDKAQLPYADIVVAGGLGLRSAENFQLVKALADVLGGEYGGSRPL
                     VQKGWITADRQIGQTGKTIRPRLYIAAGISGAIQHRVGVEGADVIVAINTDPQAPIFD
                     FAHLGIVADAVTLLPALTEAFAARLRVNRLAS"
     gene            1138..2460
                     /locus_tag="VFF98_07620"
     CDS             1138..2460
                     /locus_tag="VFF98_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU63535.1"
                     /translation="MSDTGTQTPQFDAIVVGAGPSGNAAAYVMAREGLAVLQIERGEY
                     AGSKNVQGAILYADALEQIIPDFREDAPLERHVIEQRIWTMDDRSHTGVHYRSDDFNE
                     ERPNRYTILRAQFDKWFSARVRAVGATVLFETTVTELVRDLTGRVIGVRTDRGGAPVF
                     APVVVLAEGVNGLVGQRSGLRKEVQPETVALAVKEMHFLPREVIEARFNLAGSEGVVI
                     EAMGTITRGMTGTGFLYTNEESISVGIGCIVADFATSGETPYGLLEAFKNHPSIKPLL
                     AGSEVKEYAAHLIPEGGYKAIPQLYGDGWLVVGDAGQFVNAVHREGSNLAMTTGRLAA
                     ETVIELTRKRADMTAANLARFAARLEGSFVMKDLKKYKGIPGLLHGGKAQFFGTYPRL
                     ISQAMQTWLRVDGLDKKAKEGAIARSFREARGLRGLMGDAVRLVRAWR"
     gene            2507..2803
                     /locus_tag="VFF98_07625"
     CDS             2507..2803
                     /locus_tag="VFF98_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZU63536.1"
                     /translation="MTVLSGVKVEEKLFQNRYLVDAGRPHIRVRAHDVPSAGLLAMTT
                     LCPAGCYAVNESGRVEVSADGCVECGTCRIVTQKTGEIEWNYPRGGFGVLYKFG"
     gene            2998..4782
                     /gene="nifA"
                     /locus_tag="VFF98_07630"
     CDS             2998..4782
                     /gene="nifA"
                     /locus_tag="VFF98_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HZU63537.1"
                     /translation="MPFATVPQDGARAGSAPALTAARGPHMRSGSHTRADIALRGIYE
                     ISKVLAVPGRLDAMVGNVLRLLSSFLDMHHGIVALLDEQGDPRSAVGIGWQEERIETW
                     FRCLPERVVGQIVATQMPLVIANIATSPLFADWPRSEPEAGARVSFLGVPIKDRDRVV
                     GTLTIEQVWREETPYHAADEDVRFLTMVANLIGQTVRLVDLIGRDRERLMDRQRQLEK
                     ELSRQAGEEGGRNPRGIVGNSGALRHALGQIKIVARSHSAVLLRGESGTGKELFARAT
                     HDFSPRAKKPFIKLNCAALPESVLESELFGHEKGAFTGAVSQRKGRFELADGGTLFLD
                     EIGDTSLAFQVKLLRVLQEGEFERIGGMNTIKVDVRLVCATNRNLEEMVARGEFRADL
                     YYRINVVSVRLPALRDRREDIPELAREFLRRFDEEHHTHHEFGPSAYHVLDSCYFPGN
                     VRELENCIRRTATLSRGDRILDNDFACRHDECLSATLWKSAVSQPFPIVQPRQREPIG
                     IPLPADAPPPAPPPLHEDSADRPALPLAAGDGGDFGQDNVKVSRAELVAALEATGWVQ
                     AKAARMLHLTPRQIGYALRKYGIEVKRL"
     gene            4948..>5130
                     /locus_tag="VFF98_07635"
     CDS             4948..>5130
                     /locus_tag="VFF98_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63538.1"
                     /translation="MQAGRVEAAGAGLRRAAAVLGLWLAALLLPTLLLPAAAFAQARG
                     PVVLGAASLQESLNAVA"
BASE COUNT          876 a         1584 c         1817 g          853 t
ORIGIN      
        1 caggatcgca aggaggctta cgccatggct gatgcaccca aacccgccgc cccttcggcc
       61 agcggccggg ccagcaccaa gaaggaactg ccggagcatt tccgcgccta caagcatgtg
      121 tgggtggcgg tggaaatcga gcatggcgcg gttcacccgg tcagcttcga gctgctgggc
      181 gaggggcgca agctggccga caagcgcggc gtggaactgg ccgcggtggt gatgggcgat
      241 gcgcgcgcag accttgatgt ggcggccacg cactgtttcg aatatggcgc ggatgtggtc
      301 tatatcgccc aggatccggc gctggcccat tatcgcaacg agaccgcctg cgatgtgctg
      361 acgcatctgg tcaacaccta caagcccgag atcctgctgc tgggcgccac caatctgggc
      421 cgcgatctgg cgggcgcggt ggcgacaacc ctgcaaaccg gcctcacggc tgattgcacc
      481 gagctggcga tcgacgagga aggctcgctg gcggccaccc ggcccacctt tggcggctcc
      541 ctgctgtgca ccatcttcac gctcaactat cgcccgcaga tggccaccgt gcgcccgcgg
      601 gtgctgccca tgccgccccg gcaggaaggg cgcggcggcc gtatcatcac ctacagcccg
      661 gcgatcgacg agaccagggt gatcaccaag gtgctggatt tcatcgccga ccgcgacagc
      721 gacaaggccc agctgcccta tgccgatatc gtcgtggctg gcgggctggg gctgcgctcg
      781 gccgagaatt tccagctggt gaaggcgctg gctgatgtgc tgggcggcga atatggcgga
      841 tcgcgcccgc tggtgcagaa gggctggatc accgccgacc gccagatcgg ccagaccggc
      901 aagacgatcc gcccgcgcct ctatatcgcc gccggcattt ccggcgccat ccagcaccgg
      961 gtgggggtgg aaggggccga tgtgatcgtg gccatcaaca ccgatccgca ggcgccgatc
     1021 ttcgattttg cccatctggg gatcgttgcc gatgccgtca cccttctgcc cgcgttgacc
     1081 gaggcctttg ccgcccggct gcgggtcaac cgtctggcca gctgagagga gcgcgccatg
     1141 agcgacaccg gaacccaaac gccgcaattc gatgccatcg tggtgggggc ggggccttcg
     1201 ggcaatgccg cggcctatgt gatggcccgc gaagggctgg ccgtgctgca gatcgagcgc
     1261 ggcgaatatg ccggcagcaa gaatgtgcag ggggccatcc tctatgccga tgcgctggag
     1321 cagatcatcc ccgatttccg cgaggatgcg ccgctggaac gccatgtgat cgagcagcgg
     1381 atctggacga tggacgacag gagccacacc ggcgtccatt accgctccga cgatttcaac
     1441 gaggagcggc ccaaccgcta caccatcctg cgcgcccagt tcgacaaatg gttcagcgcc
     1501 cgggtgcgcg cggtgggcgc cacggtgctg ttcgagacga cggtgacaga gctggtgcgc
     1561 gacctcaccg gccgggtgat cggcgtgcgc acggatcgcg gcggcgcgcc cgtcttcgcg
     1621 ccggtggtgg tgctggccga aggggtgaac gggctggtgg gccagcgcag cgggttgcgc
     1681 aaggaagtgc agcccgagac ggtggcgctg gcggtgaagg agatgcattt cctgccgcgc
     1741 gaggtgatcg aggcgcgctt caacctcgcc ggcagcgagg gcgtggtgat cgaggcgatg
     1801 ggcacgatca ctcgcggcat gaccggcacc ggctttctct acaccaatga ggaatcgatc
     1861 tcggtgggca tcggctgcat cgtcgccgat ttcgccacca gcggcgagac cccctatggc
     1921 ctgctggagg ccttcaagaa ccacccctcg atcaagcccc tgttggccgg cagcgaagtg
     1981 aaggaatatg ccgcccacct gatcccggaa ggcggctaca aggcgatccc gcagctttat
     2041 ggcgatggct ggctggtggt gggcgatgcc gggcagttcg tcaacgcggt tcaccgcgag
     2101 ggatcgaacc tggcgatgac caccgggcgc ctggccgccg agacggtgat cgagctgacc
     2161 cgcaagcgcg cggacatgac agcggccaac ctcgcccgct tcgccgcgcg gctggagggc
     2221 agtttcgtga tgaaggacct gaagaaatac aagggcattc ccggcctgct gcatggcggc
     2281 aaggcgcagt tcttcggcac ctatccgcgg ctgatcagcc aggcgatgca aacctggctg
     2341 cgcgtcgacg ggctggacaa gaaggccaag gaaggggcca tcgcccgttc cttccgcgag
     2401 gcgcgcgggc tgcgcgggct gatgggcgat gccgtgcggc tggtgcgcgc ctggcgctga
     2461 gccgcgaggg aaccacgaaa atggcaaggc aagaaggaga cggacgatga ctgtgttatc
     2521 cggcgtgaag gtggaggaga agctcttcca gaaccgctat ctggtcgatg ccggccggcc
     2581 gcatatccgg gtgcgcgcgc atgatgtgcc ctcggcaggc ctgctggcga tgaccaccct
     2641 gtgcccggcg ggctgctatg cggtgaatga aagcgggcgg gtggaagtgt cggccgatgg
     2701 ctgcgtggaa tgcggcacct gccgcatcgt tacgcaaaag accggggaga tcgaatggaa
     2761 ctatccgcgc ggcggctttg gcgtgcttta taaattcggt tgaattcagg cactccgcca
     2821 gctgttgtgc ggttgttgcc ggaaaaccgt atatggcgga atatagacca tttttcttgc
     2881 cgctatagag ggtttttgag aaattatgac aagcggaaaa aatgattcgc gccgccattt
     2941 ccaggcaggg gcgaacgatc cgggaaagca tgcgctgatg gcaggaaggt tggccccatg
     3001 ccttttgcca cagttcccca ggatggcgcg cgcgccggat cggcgccggc gctgacggct
     3061 gcgcgcggcc cccacatgcg cagcggcagc cacacccgcg ccgatatcgc cctgcgcggt
     3121 atctacgaga tctccaaggt tctggcggtg ccggggcggc tggatgcgat ggtgggcaat
     3181 gtgctccggc tgctgtcgag ctttctcgac atgcatcacg gcatcgttgc gctgctggac
     3241 gaacagggcg atccgcgcag cgctgtcggc atcggctggc aggaggagcg gatcgagacc
     3301 tggtttcgct gcctgcccga gcgggtggtg gggcagatcg tggcgacgca gatgccgctg
     3361 gtcatcgcca atatcgccac cagcccgctg tttgccgact ggccccgcag cgagccggaa
     3421 gcgggcgcgc gcgtctcctt cctcggcgtg ccgatcaagg accgcgaccg ggtggtgggc
     3481 acgctgacca tcgagcaggt gtggcgcgag gaaaccccct atcacgcggc cgatgaggat
     3541 gtgcgcttcc tcaccatggt ggccaatctc attggccaga cggtgcggct ggtggatctg
     3601 atcggccgtg atcgggaacg gctgatggac cgccagcggc agctggaaaa ggaactgtcg
     3661 cgccaggccg gcgaggaagg ggggcgcaat ccgcgcggca ttgtgggcaa cagcggggcg
     3721 ctgcgccatg cgctggggca gatcaagatc gttgcgcgca gccattcggc ggtgttgctg
     3781 cgcggcgaaa gcggcaccgg caaggagctg ttcgcccgcg ccacccatga tttcagcccg
     3841 cgggccaaaa agcccttcat caagctgaac tgcgcggcgc tgcccgaatc ggtgctggaa
     3901 tcggaactgt tcggccatga gaagggtgcg ttcacgggcg ctgtctcgca gcgcaagggg
     3961 cggtttgaac tggccgatgg cggcaccctg ttcctcgacg agatcggcga taccagcctg
     4021 gccttccagg tgaagctgct gcgtgtgctg caggagggcg agttcgagcg cattggcggc
     4081 atgaacacca tcaaggtgga tgtgcggctg gtgtgcgcca ccaaccgcaa cctggaggaa
     4141 atggtggcgc gcggcgaatt tcgcgccgac ctgtattatc gcatcaacgt cgtttcggtg
     4201 cggctgccgg cgctgcggga tcggcgcgag gatattcccg aactggcgcg cgaattcctg
     4261 cgccgcttcg acgaggagca ccacacccac cacgagttcg gcccctcggc ctatcacgtg
     4321 ctggacagct gctatttccc cggcaacgtg cgcgagctgg aaaactgcat ccgccgcacc
     4381 gccaccctgt cgcgcgggga tcgcattctc gacaatgatt ttgcctgccg gcacgatgaa
     4441 tgcctgtcgg ccaccttgtg gaaatcggcg gtgtcgcagc ctttcccgat cgtgcagccg
     4501 cgccagcgcg agccgatcgg cattccgctg ccggcagatg cgccgccgcc cgccccgccg
     4561 cccctccacg aggacagcgc cgatcggccc gccctgccgc tggcagcggg cgatggcggg
     4621 gatttcgggc aggataatgt gaaggtcagc cgggccgagc tggtggccgc gctggaggca
     4681 accggctggg tgcaggcaaa ggctgcgcgc atgctgcatc tcaccccgcg ccagatcggc
     4741 tatgcgctgc gcaaatacgg gatcgaggtc aagcggctct agcgctgccg gggtttctgg
     4801 ccgggccggg gcgttgccgg ccattcctct ccacatcacg cgaaagggag cattgcagtg
     4861 cagcaaaagg cttgtatgcg cggcgtatcg atatacggtt ggatacaggg ggccggtgct
     4921 tccggcaggc tgaggaaagg tgtggcgatg caggcagggc gggtagaagc ggcaggggcc
     4981 gggctgaggc gcgcggcggc ggtgctgggc ttgtggctgg cggccctgtt gctgccgacc
     5041 ctgttgctac cggcggcggc ctttgcgcag gcgcgcgggc cggtggtgct gggggcggcc
     5101 tccttgcagg aatcgctgaa tgccgtggcc
//
LOCUS       DASRYE010000142         9426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_23596,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000142 DASRYE010000000
VERSION     DASRYE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23596"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFF98_07640"
     CDS             complement(<1..776)
                     /locus_tag="VFF98_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZU63539.1"
                     /translation="MTSEEIQAIFFVECEESLAAAEQGLAACKAGTQDADTINAVFRG
                     VHSIKGGAGAFGFAALQAYTHVFETLLSDVREGLVAITPPLVDLLLRALDVLADHVTA
                     AREATPAPEDAALLAEMEASMAKNAGGAPAPAAAPAPAPAPAASAEPEDEGFDIDLDA
                     LLDDLAGPAAAPAAAEGWQVHLRPRAGAMRNGSEPLLLLRELAGLGAVCTTCDTSAIP
                     PLDDFDPHQGYLGWTFELPAEVSDGPVRDIFDFVGEDCPIA"
     gene            complement(773..1033)
                     /locus_tag="VFF98_07645"
     CDS             complement(773..1033)
                     /locus_tag="VFF98_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZU63540.1"
                     /translation="MPTIQLPARCDRAATEALLPEFQAAQGSGVLSIDAAACQQVGQA
                     MLQLLASARRSGDGAQITPSPALLEAARLTGLETLLFDGGEA"
     gene            complement(1205..3091)
                     /locus_tag="VFF98_07650"
     CDS             complement(1205..3091)
                     /locus_tag="VFF98_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU63541.1"
                     /translation="MAGTAIVSALVLGVVAGGFGINQIRYGGPLHHRNQQVNDLVADI
                     LPPPNYVIEPFLEATILAQNPQTLPTHRDRLKQLEDQFQQEAKRWQGADLDDDLKQRL
                     FTQSDVTARQFWAELDNNFLPAIEQNQPQRMTDSYRQLAAIYATHRAQIDALVTASTN
                     RQNELNQSSGLTLTLTFVGLAGMAGLVLAMVMGANRYLRGQVLAPLGETAETMRRMAA
                     GDLDATRGPTAREDELGDMARAIAVFRDNALRERATMAEQADLLAKLTAAFEQLATGQ
                     LDYRIDSPFPPAQEPLREAFNETMEDLADMFGGVTRSARQVNRGTQEIRAASDDLARR
                     NEQHAASLKEALMAMEQMTEIIRESADSAGQVQQSIAKAHVEAEEGGEVVSRATEAMA
                     AIEQSAKQISQIITVIDGISFQTNLLALNAGVEAARAGEAGRGFAVVANEVRALAQRA
                     ADAAKDIRRLITTSSDQVNTGVGLVKATGDLLGQISAHVGEVNQMIDGIAAYANMQAS
                     TVGMVNGSLMQMENVTEQNAAMSEEAHAAAMSLAQEAASLAELAARFGVGEEAGFAPA
                     HALTHAPAYTPAPRARALPAPAAEVTPFLPPRRAAPALPAPLASQGNLALKAEVEDSD
                     WSSF"
     gene            3302..4363
                     /locus_tag="VFF98_07655"
     CDS             3302..4363
                     /locus_tag="VFF98_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZU63542.1"
                     /translation="MISESGTAMHEARVLVVDDSAAMRALFSDVLEQSRNVTVVGTAA
                     SAAEARDQIAELRPNVVTLDVEMPGMSGIEFLEELMTTQPLPVVMLSSLTQSGTEISL
                     KAYALGAVECFPKPLKATPEQFAKTVGKLGKIVLAAANSNVRERKSGGSARREAAAQA
                     QFTWNGHILAMSASMGGIDALSQLLADFPAECPPTVITLASEPALVDTFIMRLSGELA
                     CKIVPAQDGAALVPGTIHIAADPERHVIFEPGQPPKLRMITRDPVEGARPSATLLYGT
                     LARAGVPAVGVVLTGMGEDGARGLRLMRDAGCRTFAQDRATATVAEAPAAAVAANAVE
                     KELPLEGLGEAILGCCNQA"
     gene            complement(4418..5020)
                     /locus_tag="VFF98_07660"
     CDS             complement(4418..5020)
                     /locus_tag="VFF98_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZU63543.1"
                     /translation="MSTGTSLFLVDGDVRRRAAISHCLVGSTIHVEPFEDASELISRW
                     PRQGLLLVHDDSRAIGTLLGQMGRSGQWLPVIGFAQEPTTRMVVRAVLDGAIDYIAWP
                     FDREDIIAVVREAEAKAETLGSTKLREAMARSRVDRLTKREREVLAGVAGGLSNRMIG
                     EKLSISPRTVEIHRANMLTKMGANHTSEAIRIAIEAALVD"
     gene            complement(5321..5695)
                     /locus_tag="VFF98_07665"
     CDS             complement(5321..5695)
                     /locus_tag="VFF98_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HZU63544.1"
                     /translation="MLASRDPNQVYRRVDFDARVTAADPAELVKLCFEQLIAAIGSAL
                     IAEERGDNQTKSRALTRAISALTALQLGVSGNDSMSQALLVLYNAARKSVLDSVLAFH
                     APTLERIRADFIEIAQSLTGAA"
     gene            complement(5709..7268)
                     /gene="fliD"
                     /locus_tag="VFF98_07670"
     CDS             complement(5709..7268)
                     /gene="fliD"
                     /locus_tag="VFF98_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZU63545.1"
                     /translation="MTSTSSTSSTTSSTASLVSALSSTVANTTATAASTTSTSIGAQL
                     LTSLGVGTGINMDTLATEMATAQYATATTNVTNQLSAVSVEISDATTLKGYMSSLTSS
                     FYSLVSAGSLASSPTVTNSQVASATLPEGQSGASTSYSLEVSQLAQSQVLASSSFSSS
                     SATTGSGTLSIQFGTATAATSTAAASFTADSSKSAVSITVNQGDSLATIAQSINNSGA
                     GLSAYVSSNADGTAQLVLKGPTGADNAFTITASENSADPGLSALNWNPASTSSTMSSA
                     QGAQDASYKLDGISHTSASNTIANAAPGLSLSLTGTNAGAPTTITYSDPSSAITTAMT
                     NLTSTLNQIITTMNGYMTPSTSGSLANDPGAMALQTALTQLPGETIMPNATGTAPATL
                     ADLGLTLAKDGSLTLDTTKLSSILTSNPSGVAAMFTTGLNGIYGTLFNISSAVSSSTD
                     PGSLEGSITYYTAQQTTLQSQQTAIATQQASLRTQLLSQLTNADTAVATSNSTLTFLK
                     NQIAAWNKTSN"
     gene            complement(7299..8495)
                     /locus_tag="VFF98_07675"
     CDS             complement(7299..8495)
                     /locus_tag="VFF98_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZU63546.1"
                     /translation="MAETAGEKTLAPTEKRLKDAAKDGDVLRSKELSIAAATLTGAAI
                     LRAGGPWLFSVLERTMRSGLVWDRAGIDAFDPGRVMLGLMALLLPAVAAVGLAMVAVS
                     IGAQLGPSGRGRFNPGNLAPKPQRLNPASGLSRIFGPQGWIEVGKGLLKVGLLGGIAW
                     GWASSRLDGLARLGGSGLAGQLAFGWDAILSLMFQLSGGLIIIALIDFPIQWLRRQRR
                     LRMSLQEVRDEHKEQDGSPEAKGARRNRQRQIAMGAIAAAMRKAQFVITNPTHFAVAL
                     TYDPDLAPAPVVLAKGRGEKALAIRELAAEHKLPCLEYPALARSVYYTTRERQMIREE
                     LYAAVAGVVAFVLSLERGEKRPAPVIDVPLELRFDAFGKPAADSGGQATPGSAEKTDE
                     TTPAAP"
     gene            complement(8500..9282)
                     /gene="fliR"
                     /locus_tag="VFF98_07680"
     CDS             complement(8500..9282)
                     /gene="fliR"
                     /locus_tag="VFF98_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZU63547.1"
                     /translation="MPGLHFGFGALEADFWRWAFLMTRIGAALVGVPFFSLGNVPTEV
                     RVILAAAVAALVAGWTGAAAPADLLSLAGMLAVAGEVLVGLMLGFVLQIAFAGPVIAA
                     ELIGGGMGMGLAASVDPTNGAHSPALGQFFGVILTLIFLGLGGHLQWFALLVRSYDTF
                     PPGHTWLGPEHFRAVAGFASAMFATAVAMALPVSLVLLLVQLMAGILSRSAPALNLFS
                     LGLPAAICAGFAALLASLPLLGDRLTTLSADAIAACASLTGG"
     gene            complement(9287..9415)
                     /locus_tag="VFF98_07685"
     CDS             complement(9287..9415)
                     /locus_tag="VFF98_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63548.1"
                     /translation="MPKLIATALVLILFGASILGLIGDFTKDIFAAIARLGSNQGL"
BASE COUNT         1457 a         3069 c         3311 g         1589 t
ORIGIN      
        1 gcgatcgggc aatcctcgcc gacaaagtcg aaaatgtcgc gcacggggcc gtcgctcacc
       61 tcggcgggca gttcgaaggt ccagccgaga tagccctgat gcgggtcgaa atcgtcgagc
      121 ggggggatcg cggacgtgtc gcaggtggtg cacaccgcgc caagcccggc cagttcgcgc
      181 agcagcagca gcggttcgct gccattgcgc atggccccgg cgcgcgggcg cagatgcact
      241 tgccagccct cggcggcagc gggcgcggcg gcagggccgg ccagatcgtc gagcagggca
      301 tcgaggtcga tgtcgaagcc ttcgtcttcc ggctcggcgc tggcggcggg ggcaggggca
      361 ggcgctgggg ccgcagcagg cgccggggcg ccgccggcgt tcttggccat cgaggcttcc
      421 atctcggcca gcagcgcggc atcctcgggc gccggggtgg cctcgcgggc ggcggtcaca
      481 tggtcggcca gcacatccag cgcgcgcagc agcagatcca ccagcggcgg ggtgatcgcc
      541 accagcccct cgcgcacatc agacagcagc gtttcgaaca catgcgtata ggcctgcagc
      601 gcggcaaagc cgaaagcgcc cgcgccgccc ttgatggaat gcacgccgcg aaacaccgcg
      661 ttgatcgtgt cggcatcctg ggtgccggcc ttgcaggcgg ccaggccctg ttcggccgcc
      721 gccagcgatt cctcgcattc gacgaagaag atcgcctgga tttcttcgct cgtcatgcct
      781 cgcctccgtc gaacagcagc gtttcgaggc cggtcagccg ggccgcttcc agcagcgccg
      841 gcgacggggt gatctgggcg ccatcgccgc tgcgccgggc gctggccagc agttgcagca
      901 tggcctggcc cacttgctgg caggcggccg catcaatgga gagcacgccg ctgccctgcg
      961 ccgcctggaa ttcaggcagc agggcctcgg tggcggcgcg atcgcatcgg gccggcaatt
     1021 ggatcgtggg catcggccaa aactctcctc tctggcgccc tggcagggca gtgcggcact
     1081 tggggcggcg gatctgccgc gggcctgccg ccgcggcggg cacaccggtc ttacgctggg
     1141 ggggcaaatt catcgcttgc gcggatctca gtaatttccc gggaaaatgc cggggcgggg
     1201 cgcgtcagaa gctgctccag tcgctgtcct caacctcggc cttcagcgcc agattgccct
     1261 ggctggccag cggcgccggc agggcgggcg cggcgcggcg cggcggcagg aagggggtca
     1321 cctcggcggc cggggccggc agcgcgcggg cgcgcggcgc gggggtgtag gcgggcgcat
     1381 gggtcagcgc atgggcgggc gcaaagccgg cctcctcgcc cacgccaaag cgcgcggcca
     1441 gttcggccag gctggccgct tcctgcgcca ggctcatcgc tgccgcatgg gcctcttccg
     1501 acatggcggc attctgctcg gtcacgtttt ccatctgcat cagcgagccg ttgaccatgc
     1561 cgacggtgga ggcctgcatg ttggcatagg cagcgatgcc gtcgatcatc tggttgacct
     1621 cgccgacatg cgccgagatc tgccccagca gatcgccggt ggccttcacc aggccaacgc
     1681 cggtgttcac ctgatcgctg gaggtggtga tcagccggcg gatatccttg gcggcatcgg
     1741 cggcgcgctg ggccagcgcg cgcacctcgt tggccaccac cgcaaagccg cgcccggcct
     1801 cgccggcgcg ggccgcttcc accccggcgt tcagcgccag caggttggtc tggaacgaga
     1861 tgccatcgat cacggtgatg atctggctga tctgcttggc cgattgctcg atcgcggcca
     1921 tcgcctcggt cgcgcgcgag acgacctcgc cgccttcctc ggcctccaca tgggccttgg
     1981 cgatgctttg ctggacctgg ccggcgctgt cggcgctttc gcggatgatc tcggtcatct
     2041 gctccatcgc catcagcgct tccttcagcg aggcggcgtg ctgttcgttg cggcgggcca
     2101 gatcatcgga agcggcgcgg atttcctgcg tgccgcgatt gacctgccgg gccgagcggg
     2161 tgaccccgcc gaacatgtcg gccagatctt ccatcgtctc gttgaaggcc tcgcgcaggg
     2221 gttcctgggc gggcgggaag gggctgtcga tgcgatagtc gagctggcct gtggccagtt
     2281 gctcgaaagc ggcggtcagc ttggccagca ggtcggcctg ctcggccatg gtggcccttt
     2341 cgcgcagggc attgtcgcgg aacacggcga tggcgcgggc catgtcgccc agctcgtcct
     2401 cgcgggcggt ggggcccctt gtcgcatcga gatcgcctgc ggccatgcgc cgcatcgtct
     2461 cggccgtctc gcccagcggc gccagcacct ggccgcgcag atagcggttg gcccccatca
     2521 ccatggccag caccagcccg gccatgcccg ccagccccac gaaggtgagc gtgagggtga
     2581 ggccgctgga ctggttcagc tcgttctggc ggttcgtcga ggcggtgacg agcgcgtcga
     2641 tctgggcgcg gtgggtggca tagatcgccg ccagctggcg atagctgtcg gtcatgcgct
     2701 ggggctggtt ctgttcgatg gcgggcagga aattattgtc gagttcggcc cagaactggc
     2761 gcgcggtcac gtcagactgg gtgaacagcc gctgcttcag atcgtcgtca agatctgccc
     2821 cctgccagcg tttggcttcc tgctggaact ggtcttccag ctgcttcagc cggtcgcggt
     2881 gggtgggcag ggtttgcggg ttctgcgcca ggatcgtggc ttcgaggaag ggctcgatca
     2941 cataattggg cggcggcaga atatcggcga caaggtcgtt cacctgctgg ttgcggtggt
     3001 gcagcggccc gccatagcgg atctggttga tgccaaagcc gcctgccacc acgcccagca
     3061 ccagcgccga gacgatcgcg gtgcccgcca tcctgctttt ttgctcaatc ctcatcggca
     3121 tttcccgtaa cggcggtttc ccgcgccacc cctgtatcgg ggccatcgcc tatcgattag
     3181 acgaggagtg cgaagcgccg cttgaagcac gtctggggag atatacgggg gtgggggcag
     3241 gcagttcttt gcctttgttt gctaggtgct tggcttgtgc ccgccaggcc gggcccgatg
     3301 tttgatcagc gagagtggca cggcgatgca tgaagcgaga gttctggtgg tcgatgattc
     3361 ggcagcgatg cgcgcattgt tttccgatgt gctcgaacag tccaggaacg tcaccgtggt
     3421 gggcacggct gccagtgccg ccgaagcgcg cgaccagatc gccgagctgc gccccaatgt
     3481 ggtgacgctg gatgtggaaa tgcccggcat gagcggcatc gaattcctgg aagagctgat
     3541 gaccacccag ccgctgccgg tggtgatgct gtcctcgctc acccagtcgg gcacggaaat
     3601 ctcgctcaag gcctatgcgc tgggggcggt ggaatgtttc cccaaaccgc tgaaggccac
     3661 gcccgagcaa tttgccaaaa cggtgggcaa gctgggcaag atcgtgctgg ccgcggccaa
     3721 ttccaacgtg cgcgaacgca agagcggtgg cagcgcccgc cgcgaagccg ccgcccaggc
     3781 ccagttcacc tggaacggcc atatcctggc gatgagcgcc tcgatgggcg ggatcgacgc
     3841 gctgagccag cttttggccg atttccccgc cgaatgcccg ccaaccgtga tcaccctcgc
     3901 cagcgagccg gcgctggtgg atacgttcat catgcgcctc tcgggcgagc tggcctgcaa
     3961 gatcgtgccc gcccaggatg gcgccgcgct ggtgcccggc accatccaca tcgccgccga
     4021 tcccgaacgc catgtgatct tcgagccggg ccagccgccc aagctgcgca tgatcacccg
     4081 cgatccggtg gaaggggcgc gcccgtctgc cacgctgctg tatggcacgc tggcgcgcgc
     4141 gggcgtgccg gcggtgggcg tggtgctcac cggcatgggc gaggatggcg cgcgcggcct
     4201 gcggctgatg cgcgatgccg gctgccgcac tttcgcgcag gatcgcgcca ccgccaccgt
     4261 ggccgaagcg ccggcagcgg ccgtggccgc caatgcggtg gaaaaggaat tgccgctgga
     4321 agggctgggc gaggcgattc tgggctgctg caaccaggcc tgaggccgcg ggggcggggc
     4381 cgatgcccgc cccggccctg ccagttggca ccccgcctta atcgaccagc gcggcctcga
     4441 tggcgatgcg gatcgcctcc gatgtgtggt tggcgcccat tttcgtcagc atgttggcgc
     4501 ggtggatttc caccgtgcgc ggcgagatcg acagtttctc gccgatcatc cggttggaaa
     4561 ggccgccggc cacgccggcc agcacttcgc gttcgcgctt ggtcagccgg tccacccggc
     4621 tgcgggccat ggcctcgcgc agcttggtgc tgcccagcgt ttcggccttg gcttcggcct
     4681 cgcgcaccac ggcgatgata tcctcgcgat cgaacggcca ggcgatatag tctatcgcgc
     4741 catccagcac cgcgcgcacc accatgcggg tggtgggttc ctgcgcgaag ccgatcaccg
     4801 gcagccactg gcccgagcgg cccatctgcc ccagcagggt gccgatcgcg cggctgtcgt
     4861 catgcaccag cagcaggccc tggcggggcc agcggctgat gagttcggaa gcatcctcaa
     4921 acggttcgac atggatcgtg ctgccgacca ggcaatggga aatggccgcc ctgcgccgca
     4981 catcaccatc gacaaggaaa aggctggttc ctgtgctcaa agttgatatc cccctcttgc
     5041 ccttggttta tgcctagcaa atactgccta ggattggagt aataactgtc tccgtttcga
     5101 cgtagctttc gatccgaaat agattcattg cctgtaatat aagtatttgt gcctattcat
     5161 gagaaataag tcgctgtcgc caaggcttgc gccccgccgc aggccgggca caacagtatc
     5221 ggcaaaggca ggggcaaggc ggcatgcgct tgcgcgccgg cgttaaccag cgcgctgcgg
     5281 ggcacaaaac aggagcgcaa ggggccgttc ggcggcctgt tcaggcagcg ccggtcaggc
     5341 tctgggcgat ttcgatgaaa tcggcgcgaa tgcgctccag cgtcggcgca tgaaaggcca
     5401 gcacactgtc gagcacggat ttgcgcgccg cattatacag caccagcagc gcctgcgaca
     5461 tcgaatcatt gcccgaaacg cccagctgta gcgccgtcag cgccgagatc gcgcgggtga
     5521 gggcgcggct cttggtctgg ttgtcgccgc gttcctcggc gatcagcgcg ctgccgatcg
     5581 cggcgatcag ctgctcgaag cacagcttca ccagttcggc cgggtcggcg gcggtcaccc
     5641 gggcgtcgaa atcgacgcgg cggtagacct ggttgggatc gcggctggca agcatggctg
     5701 ttccctcctc aattgctcgt cttgttccag gcggcgatct ggttcttcag gaaggtcagc
     5761 gtcgaattcg acgtggcgac ggcggtatcg gcgttggtca gctggctcag caattgcgtg
     5821 cgcaaactgg cctgctgggt ggcaatggcc gtttgctggc tttgcagggt ggtctgctgg
     5881 gcggtgtaat aggtgatcga gccctccagc gagccggggt cggtggagga actgaccgcg
     5941 ctcgatatgt tgaacagcgt gccatagatg ccattcagcc cggttgtgaa catggccgcc
     6001 acgccgctgg ggttgctggt cagaatgctc gacagcttgg tggtgtcgag cgtgaggctg
     6061 ccatccttgg ccagcgtcag gcccagatcg gccagcgttg caggggcggt gccggtggca
     6121 ttgggcatga tcgtttcgcc cggcagctgg gtcagcgcgg tttgcagcgc catcgcgccg
     6181 ggatcattgg ccagcgagcc cgaggtggag ggggtcatgt agccgttcat cgtggtgatg
     6241 atctggttga gcgtgctggt caggttcgtc atcgcggtgg tgatcgcgct cgacgggtcg
     6301 gaataggtga tggtggtggg cgcgccggcg ttggtgccgg tcaggctcag cgagaggccg
     6361 ggggcggcat tggcgatggt gttgctggcg ctggtgtggc tgatgccatc cagcttgtag
     6421 ctggcatcct gcgcgccctg ggccgaggac atcgtgctgc tggtgctggc cgggttccag
     6481 ttcagcgcgg aaaggcccgg atcggcgctg ttctcgctgg cggtgatggt gaaggcgttg
     6541 tcggcgccgg tggggccttt cagcaccagc tgggcggtgc catcggcatt gctgctgaca
     6601 taggcggaca ggccggcgcc ggaattgttg atgctctggg cgatggtggc caggctgtcg
     6661 ccctggttga cggtgatgct caccgcgctc ttgctgctgt cggcggtgaa gctggcggcg
     6721 gcggtgctgg tggcggcggt cgccgtgccg aactggatcg acagcgtgcc cgagccggtg
     6781 gtggccgagg aagaggaaaa gctggaggag gccagcacct gcgactgggc cagctggctc
     6841 acttccagcg aatagctggt gctggcgccg ctttggcctt cgggcagggt ggcgctggca
     6901 acctggctgt tggtaaccgt tggcgaggag gccagcgagc cggcgctgac cagcgaatag
     6961 aacgagctgg tgagcgagga catatagccc ttcagcgtcg tcgcgtcgga aatctctacc
     7021 gagacggcgc tgagctggtt ggtgacgttg gtggtggccg tggcatattg ggcggtggcc
     7081 atttcggtgg ccagcgtatc catgttgatg cccgtgccca cgcccagcga ggtcagcagc
     7141 tgggcgccga tcgaggtgct ggtggtgctg gccgcggtgg cggtggtgtt ggcaaccgtc
     7201 gagctcagcg cgctgacaag gctcgctgtc gagctggtgg tgctgctggt ggacgaggtg
     7261 ctggtcatga gaggaagaac gtctcgcagg cgccggggtt aaggggcggc aggggtggtt
     7321 tcatcggtct tttcggcgct gcccggggtg gcttgcccgc cgctgtcggc cgccggcttg
     7381 ccaaaggcat cgaagcgcag ctccagcggc acgtcgatca ccggggcggg gcgcttttcc
     7441 ccccgttcca gcgagagcac gaaggcgacc acgcccgcca ccgccgcata gagttcctcg
     7501 cggatcatct gccgctcgcg cgtggtgtag tagaccgagc gggccagcgc cggatattcc
     7561 aggcagggca gcttgtgctc ggccgccagc tcgcggatcg ccagcgcctt ctcgccgcgc
     7621 cccttggcca gcaccaccgg cgccggcgcc agatccggat cataggtgag cgccacggcg
     7681 aaatgggtgg ggttggtgat gacgaactgc gccttgcgca tggccgcggc aatcgcgccc
     7741 atggcgatct ggcgctggcg gttgcggcgg gcgcccttgg cctcggggct gccgtcctgc
     7801 tccttgtgct cgtcgcgcac ttcctgcagg ctcatgcgca ggcggcgctg gcggcgcagc
     7861 cactggatgg gaaaatcgat cagggcgatg atgatcagcc cgcccgacag ctggaacatc
     7921 aggctgagga tcgcatccca gccgaaggcc agctggccgg ccagcccgct gccccccagc
     7981 cgcgccagcc catccagccg cgaagaggcc cagccccagg cgatgccgcc cagcaggccc
     8041 actttcagca ggcccttgcc cacctctatc cacccctgcg gaccgaaaat gcgcgacagg
     8101 ccgctggccg ggttcagccg ctggggcttg ggcgccagat tgccggggtt gaagcgcccc
     8161 cgccccgaag ggcccagctg cgccccgatg ctgaccgcca ccatcgccag ccccaccgcc
     8221 gccaccgccg gcagcagcag cgccatcagc cccagcatca cccggccggg atcgaaagcg
     8281 tcgatcccgg cgcgatccca caccaggccc gaacgcatgg tgcgctccag cacggagaac
     8341 agccagggcc cgccggcgcg caggatggca gcgccagtca gcgtggccgc ggcaatgctc
     8401 agctctttgg agcgcaggac atcgccgtcc ttcgctgcgt ctttcagccg cttttcggtg
     8461 ggggcaaggg ttttctcgcc ggcggtttcg gccatcggct cagccccccg tcaggctggc
     8521 gcaggcggca atggcatcgg ccgacagcgt ggtcagccgg tcgcccagca gcggcaggct
     8581 ggccagcagc gcggcaaaac cggcgcagat cgccgctggc agccccagcg aaaacaggtt
     8641 cagcgccggc gccgagcgcg acaggatgcc cgccatcaac tgcaccagca gcagcaccag
     8701 gctgaccggc agcgccatcg ccacggcggt ggcgaacatg gcgctggcaa agccggccac
     8761 cgcgcggaaa tgctctggcc ccagccaggt gtggccgggg ggaaaggtgt cgtagctgcg
     8821 caccagcagc gcgaaccatt gcagatggcc gccaaggccc aggaagatga gcgtgaggat
     8881 caccccgaaa aactgcccca gcgccggcga atgggcgcca ttggtgggat cgacactggc
     8941 cgccagcccc atccccatgc cgccgccgat cagctctgcc gcgatgacag ggccggcaaa
     9001 ggcgatctgc agcacaaagc ccagcatcag ccccaccagc acctcgcccg ccaccgccag
     9061 catgcccgcc agcgagagca ggtctgccgg cgctgcggcc cccgtccacc cggcgaccag
     9121 cgccgccacg gctgcggcca ggatcacccg cacctcggtt ggcacattgc cgaggctgaa
     9181 gaagggcact cccaccagcg ccgcgccgat ccgcgtcatc aggaaggccc agcgccagaa
     9241 atccgcttcc agcgcgccaa agccgaaatg caggcccggc atcgccctaa agcccctgat
     9301 tgctgccaag ccgcgcgatg gcggcgaaga tatccttcgt gaaatcgccg atcagcccca
     9361 atatgctggc gccaaacagg atcagcacca gcgcggtggc gatcagcttg gggatgaagg
     9421 agagcg
//
LOCUS       DASRYE010000143        15215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_163511,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000143 DASRYE010000000
VERSION     DASRYE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15215
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163511"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /gene="dxs"
                     /locus_tag="VFF98_07690"
     CDS             complement(<1..1859)
                     /gene="dxs"
                     /locus_tag="VFF98_07690"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZU63549.1"
                     /translation="MTREKPYSQLDRIATPSDLRGLSVSELVELAGEVRDEMIHSVGQ
                     TGGHLGSGLGVVELTVALHYVFETPRDKIVWDVGHQAYPHKILTGRRGQMRTIRQPGG
                     ISGFTKRSESEYDAFGAGHSSTSISAALGFAVANKIKDVPGKAIAVIGDGSMSAGMAY
                     EAMNNAAEAGNRLVVILNDNDMSIAPPVGGLSAYLARLVSSRPFLELREMAKKWSRHL
                     PGPLHRAARLTDEYARGMAMGGTLFEELGFYYVGPIDGHNLEQLVPVLENVRDAAQGP
                     CLVHVVTQKGKGYAPAEAAADKYHGVQKFDVITGEQVKPKAGPPSYTGVFAKALIAEA
                     EADDTVCAITAAMPSGTGLDKFAQQFPTRSFDVGIAEQHAVTFAAGLAAQGMKPFCAI
                     YSTFLQRAYDQVVHDVAIQKLPVRFAIDRAGLVGADGATHAGSFDITYLASLPNMVVM
                     AAADEAELVHMVHTAAAYSEGPIAFRYPRGNGTGVALPEVPQKLEIGKGRIVRGAEKG
                     GKVAILSLGTRLAEAQKAADMLEAKGLATTVADLRFAKPLDEALIRRLATTHEVVITL
                     EEGSIGGLGAHVLTLASDTGLTDAGLKIRTLRLPDTFQDHDNPDKQYDEAGLTA"
     gene            1995..2492
                     /locus_tag="VFF98_07695"
     CDS             1995..2492
                     /locus_tag="VFF98_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZU63550.1"
                     /translation="MTALIVNGRPLDFRMDPQVPLLWALREGANLTGTKYGCGAGDCG
                     ACTVLADGAALKSCQISLAQAEGRRITTIEGLADNGGHPVQQALLAEQAVQCGFCTGG
                     IVLAAAALLAATPAPTDAEIRAAIDNLCRCGTYPRIIRAIRRLAPAPVAPVASPTDPN
                     ERTTP"
     gene            2489..2833
                     /locus_tag="VFF98_07700"
     CDS             2489..2833
                     /locus_tag="VFF98_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HZU63551.1"
                     /translation="MKATIWHNPACGTSRKTLAILSETPGIELTVVEYLKNPPSAEKL
                     AQLYRDAGLTPQQGLRLRGTDAAERGLPEADAATVLAAMTAEPILIERPLVETEKGVA
                     LCRPQEKVLTLL"
     gene            complement(2859..3353)
                     /locus_tag="VFF98_07705"
     CDS             complement(2859..3353)
                     /locus_tag="VFF98_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZU63552.1"
                     /translation="MTDTLPAPAPDDARLLREAFRVARATLAAGSHPFGAILASADGT
                     ILMEQGNAFQPDHDMTGHAERVLMTRASIAFDRATLAGATLYTSAEPCAMCAGAAYWV
                     GVGRVVYGLAESRLKEITGDHPENPTLDLPCRVVFAAGQRQVEVVGPLLEEEAAALHE
                     GVWG"
     gene            3576..4208
                     /locus_tag="VFF98_07710"
     CDS             3576..4208
                     /locus_tag="VFF98_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZU63553.1"
                     /translation="MTTELAQSALWPRLKRHVKQAMGPWGVFLEGFFKNPVMVGSIVP
                     SSRFTIAKVLGPVDWENTKLFVEYGPGVGTFCRPVLSRLRRDAMLLVIDTNPMFIDYL
                     KATVSDSRFVAVLGSAADVEEIVRAHGHEKADYVLSGLPFSTLPPGIGAAIASATHRV
                     LRVGGAFLVYQFTPAAGHFMARFFARIDKGLEWINVPPCFLFWGWKDAEG"
     gene            complement(4234..5034)
                     /locus_tag="VFF98_07715"
     CDS             complement(4234..5034)
                     /locus_tag="VFF98_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63554.1"
                     /translation="MMKLDSSLAWRQASAAIYANRDVLLALAGVFFLLPRLAFALLAP
                     EPVAPATGAAPEEMLRQMQAYYLKVLPMLLPTVLLETVGTLAMLALFADRRRPTVREA
                     IGQGVRALLPYLAAQIGVAMAVAVAASVALALGGQQQAAGGLLLGVVLGGFAYVAVRL
                     VLLAPVVAVDGVANPLAAIRRSWALTAGNAGRLAVLLALVLVALVVVMLAATSVLGAA
                     AALALGPDGARVVSAVVESVLAAAFVVYLTGLLAAVHRQLSVQPAALF"
     gene            5179..5877
                     /gene="lipB"
                     /locus_tag="VFF98_07720"
     CDS             5179..5877
                     /gene="lipB"
                     /locus_tag="VFF98_07720"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZU63555.1"
                     /translation="MTAPSPDPQALPPAPEWRREAAPVPYRTALAEMEARNAAIAAGT
                     APELIWLLEHLPVYTAGTSAAAAEMLDPRFEVVEAGRGGRYTYHGPGQRVGYVLLDLK
                     TRARDVRGFVHALEGWVIDTLADFGVAAWRSEAGVGIWTEDIDGREAKIGAIGVRIRR
                     WVTMHGFAVNLDPDLTHFTGIVPCGIAEKGVTSLARLGKPVSAAQWDEALIARADAFL
                     ARLQQPCPPSETPA"
     gene            5874..6200
                     /locus_tag="VFF98_07725"
     CDS             5874..6200
                     /locus_tag="VFF98_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63556.1"
                     /translation="MTFIRLPLARLPLARWLAGLALAALPAACHTAPTKSDDQRTAGG
                     QVLKGSASDGMIPYEALTSEPPMAPRIAPRAAEATSATAADAATAQGTDAAPPQAPAP
                     APGFSQ"
     gene            complement(6222..7208)
                     /gene="nadA"
                     /locus_tag="VFF98_07730"
     CDS             complement(6222..7208)
                     /gene="nadA"
                     /locus_tag="VFF98_07730"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZU63557.1"
                     /translation="MTFSAASLAGLDIRAEIDRLRKERNAIILAHYYQKPELQDLADF
                     VGDSLELSRKAAETDAEVIAFCGVKFMAEVAKILSPEKTVVLPDLNAGCSLEDACPPE
                     KFRAFREAHPDHIALTYINCSTEVKALSDIIVTSSSAETILQQIPADQKIIFGPDRHL
                     GGYLARKFNRPMLLWPGVCIVHEAFSETELLKLKAQHPGAPIAAHPECPPSIVDHADY
                     VGSTSGILNYAKQMAGDTLIVATEPHIIHQMTRALPGKTFIGAPGADGNCNCNICPYM
                     ALNTLEKLYVSLRDLSPRIEIEEGLRLQAKASLDRMLEMASTSVGKGDLGAR"
     gene            complement(7205..8140)
                     /locus_tag="VFF98_07735"
     CDS             complement(7205..8140)
                     /locus_tag="VFF98_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU63558.1"
                     /translation="MGGMSLPPHPWPTGAAEQLEPLVRRVLATNPSPYTFTGTQTYLV
                     GDGDGVVVIDPGPADAAHLDALVAAIGGAPVLAIACTHTHRDHSPAAAPLAARTGAPV
                     IGCAPLVIEGDGPRLDDSFDASYAPDAVLADGEVIAGPGFALRAVATPGHTSNHLCYA
                     LEVTGEVAGEVAGAANGALFTGDHVMGWSTSVVAPPDGNMSDYLISLDRLHGREDRVY
                     YPAHGPQIDNPRQFVRSMIGHRRQRERQILTHLADGGASVQDLVGRMYKGLAPALMGA
                     AGLSVLAHLIDLEARALAWRQGDRWAATGPGGDKA"
     gene            complement(8232..8708)
                     /locus_tag="VFF98_07740"
     CDS             complement(8232..8708)
                     /locus_tag="VFF98_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HZU63559.1"
                     /translation="MSQTSDINPRIIETLYAEALVLSDEVRAAFRLAGRLDAMLAAPV
                     AGGPEDAGRIALSCEGLRTTTRVMHALAWLLNHRAFQRGELSESQLRRQGRLPADQPA
                     EPDRLALLPEEVRVLVALSCDFYARLLRLDEAWRCPDAEREGGPGALQERLRRSVA"
     gene            complement(8811..9020)
                     /locus_tag="VFF98_07745"
     CDS             complement(8811..9020)
                     /locus_tag="VFF98_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZU63560.1"
                     /translation="MTEEEMRKRLEALRVEHRDLDAAIDALSVAVNVTGGVDQLQIAR
                     LKKRKLKLKDQIAIIEDYLIPDIIA"
     gene            9592..9747
                     /locus_tag="VFF98_07750"
     CDS             9592..9747
                     /locus_tag="VFF98_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZU63561.1"
                     /translation="MEASHTSALLNKHAGLERMIQQELNRPLPDLAMVQSLKRRKLRI
                     KDALTQN"
     gene            9960..12266
                     /gene="ptsP"
                     /locus_tag="VFF98_07755"
     CDS             9960..12266
                     /gene="ptsP"
                     /locus_tag="VFF98_07755"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZU63562.1"
                     /translation="MTSAPVGPTSPSSAVTAARLILTRLHDVMASRYNAQAKLNTVVE
                     VIGESLNSEVCSIYLLREGMLELFATRGLAQEAVHVTRMAVGEGLVGSIAEHSETLNL
                     AEAAAHPDFSYRPETGEDKYHSFAGVPIVRRQRSVGVLCVQHVDPRRYEEVEIEALQT
                     VAMVLAELIANAGLIDEEDTLGGAGAQTGPEQLRGLTLVKGLGAGVAVYHQPRVQIEH
                     VVAEDIEAERQRVYLAFDKMREQIERMASQAEFGVGGEHEEVLETYRMFAYDEGWGRR
                     INEAIDSGLTAEAAIERVQQRTRMRMRQIDDPLLADRMHDLEDLSNRLLRIVSGQLGT
                     AASMGLRQDAILIARNLGPAELLEYDKRRLKGVVLEEGSLTAHVVIVARAMGIPVLGR
                     VKGLRGMVREGDPLLLDADKAVVFVRPTRDIAIAFDERFAKTRERQAGYAALRDVVPM
                     TKDGTRITVMVNAGLREDMPNLQLTGADGVGLFRTEFQFLVSATLPSRERQTRLYREV
                     MDVAGSKPVVFRTVDIGGDKVLPYLRHDGDGPAEENPAMGWRALRVALERDGLLKVQA
                     RALLEASAGRTLHVMFPMISEPWEFDAAKAVFDGQLAHLRALKKPLPEAVKFGAMLEV
                     PALADMLDVLCPRLSFLSIGTNDLTQFLFAADRAHPKLAERYDWLSPAILRFIRRIAL
                     ACQAHNVPVTVCGEMGGRQLEALALLGVGIRRLSITPAAVGPIKAMLHQVDLPAIGAA
                     MEGWLAQPPRDLRAELVAWAQGQGIVFD"
     gene            12509..13531
                     /locus_tag="VFF98_07760"
     CDS             12509..13531
                     /locus_tag="VFF98_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU63563.1"
                     /translation="MLDDTPAHTGEMLRRAREAAGLDLADVSARTRIPERHLRALEEA
                     DYTALPHRTYALGFARTYAGVLGLNAEDVIASLRRDYRGAMPEPEPTPPAFTPGDPAR
                     VPSARFAFIAGLAVLLLGVGGFAYWQGFGSDVSLPSLLPDNPPPPPPAAKPAAAPAPA
                     PSAQPTGPVVFTAQAKGIWVKFYDGDASHVLLEKQLAEGESFTLPAEAKNPMIWTGRP
                     EALAITIGGQPQPKLADAQKIMRDVPVSATALLARQAAPAAPAQPAMGPTMGPAMAPQ
                     PGMAPMPGMMTPSAPTSAAALPAPRPHRPHPHPTASSGDAAAPAAAAPAPVAAPAPAQ
                     PSTVSQ"
     gene            13668..14687
                     /locus_tag="VFF98_07765"
     CDS             13668..14687
                     /locus_tag="VFF98_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63564.1"
                     /translation="MTSTPRRARGLLAAGPLMALALMLAGTLVTTATPAHAEDSSDQE
                     VRLRKLEAEVRALQRQVFPGGDRKFFPQDATPQTGPAAAPQPGMPATTPITDLLSRMD
                     AIEAQNTRLTSQNEELANRVRILEAARAQALSAEAPPPAPPPAVGYAPSYGPAATPAP
                     AAAPAPRPAARPAPVDGDLGGQRAPAAGPIARPSAQRLAGVQAIIKPQTGDAGEDEYT
                     YGFKLWEAKFYPEAEQQLKLFLDHYPRHRRSSYARNLLGRAYLDEGKPREAAPWFLQN
                     YQGDKKGDRAADSLLYLAETMVKMKDTNRACIALAEFADTYKTEAAGRLQGQYNSTTA
                     LAKCN"
     gene            14732..>15215
                     /gene="tilS"
                     /locus_tag="VFF98_07770"
     CDS             14732..>15215
                     /gene="tilS"
                     /locus_tag="VFF98_07770"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZU63565.1"
                     /translation="MTAELARLWPEGARQGRLGLAVSGGPDSLALLLLAHAAWPGRVA
                     AATVDHGLRPESAGEAAEVARICAGLGVAHATLPVSIGPGNIQTEARTARYAALAQWM
                     AAEGLAALATAHHADDQAETLLLRLNRASGVAGLAGVRARALVPGSRLLLLRPLLGWR
                     R"
BASE COUNT         2426 a         5399 c         5089 g         2301 t
ORIGIN      
        1 gcggtgaggc cggcttcgtc gtattgcttg tcggggttgt cgtggtcctg gaaggtgtcg
       61 ggcaggcgca gggtgcggat cttgaggccg gcgtcggtaa ggccggtgtc ggaggccagc
      121 gtcagcacat gggcgcccag accgccgatg ctgccctcct ccagcgtgat caccacctca
      181 tgggtggtgg ccaggcggcg gatcagcgcc tcgtccagcg gcttggcaaa cctgaggtcg
      241 gcgaccgtgg tggccaggcc cttggcttcc agcatgtcgg ccgccttctg cgcctcggcc
      301 aggcgcgtgc ccaggctgag gatcgccacc ttgccgccct tctcggcccc gcgcacgatg
      361 cggcccttgc cgatctccag cttctgcggc acctcgggca gggcaacccc ggtgccattg
      421 ccgcgcggat agcgaaaggc gatcggcccc tcactatagg cagcggcggt gtgcaccata
      481 tgcaccagtt cggcctcatc ggccgcggcc atcaccacca tattgggcag gctggccaga
      541 taggtgatgt cgaaagagcc ggcatgggtg gcgccatcgg cccccaccag cccggcccgg
      601 tcgatcgcaa agcgcaccgg cagcttctgg atcgccacat catgcaccac ctgatcgtag
      661 gcgcgctgca ggaaggtgga atagatcgcg cagaagggct tcatgccctg cgcggcgagc
      721 cccgcggcaa aggtcaccgc atgctgctcg gcaatgccga catcgaagct gcgggtggga
      781 aactgctggg caaacttgtc gagcccggtg cccgagggca tcgccgcggt gatcgcgcag
      841 accgtatcat cggcctcggc ctcggcgatc agcgccttgg cgaacacgcc ggtgtagctg
      901 ggcggcccgg ccttgggctt cacctgctcg ccggtgatga catcgaactt ctgcacgcca
      961 tgatacttgt cggcggcagc ttcggccggc gcatagccct tgcccttctg cgtgacgaca
     1021 tggaccaggc agggcccctg cgctgcatcg cgcacatttt ccagcaccgg caccaattgt
     1081 tccagattgt gcccgtcgat cggccccaca tagtagaagc ccagctcctc gaacagcgtg
     1141 ccgcccatcg ccatgccgcg ggcatattcg tcggtcagcc gggcggcgcg gtgcagcggg
     1201 ccgggcagat ggcgcgacca cttcttggcc atctcgcgca gttccaggaa cggccgcgag
     1261 gacaccagcc gcgccagata ggccgacaag ccccccaccg gcggcgcgat cgacatgtcg
     1321 ttgtcgttca ggatcaccac cagccggttg cccgcctcgg cggcattgtt catcgcctca
     1381 taggccatgc ccgccgacat cgagccatcg ccgatcaccg cgatcgcctt gcccggcaca
     1441 tccttgatct tgttggccac cgcaaagccc agcgccgccg agatcgaggt ggaggagtgc
     1501 cctgcgccaa aagcgtcata ttcgctctcg ctgcgcttgg tgaagccgga aatgccgccc
     1561 ggctggcgga tcgtgcgcat ctgcccgcgc cgaccggtga ggatcttgtg cggataggcc
     1621 tggtgcccca cgtcccacac gatcttgtcg cgcggcgtct cgaacacata gtgcagcgcc
     1681 accgtcagct ccacaacgcc aaggccggag cccagatggc cccccgtctg ccccaccgag
     1741 tggatcatct catcacgcac ctcgcccgcc agctcaacca gctccgatac actcaaaccc
     1801 cgaaggtccg acggcgtggc gatccggtcg agttgcgaat acggcttttc cctggtcatg
     1861 gcctcgcgcc ttacacatca ctgccgcacc tgtcatcact tgcgcgcgag aatgttgccc
     1921 tgtggtgaac gcggtgggga accgggcatc gccgcccttg ccgccctgcc cgcgctggtt
     1981 ctaaagcggg cggcatgacc gcgctgatcg tcaatggccg cccgctggat ttccgcatgg
     2041 atccgcaagt gccgctgctg tgggcgctgc gcgagggggc caatcttacc ggcaccaaat
     2101 atggctgcgg ggcgggcgat tgcggcgcct gcacggtgct ggccgatggc gccgcgctga
     2161 aatcctgcca gatcagcctg gcccaggccg aggggcggcg catcaccacc atcgaggggc
     2221 tggccgacaa tggcggccac ccggtgcagc aggcgctgct ggccgagcag gcggtgcaat
     2281 gcggcttttg caccggcggc atcgtgctgg cggccgcggc gctgctggcg gcaacccctg
     2341 cccccaccga tgccgagatc cgcgccgcga tcgacaatct gtgccgctgc ggcacctatc
     2401 cgcgaatcat ccgggcgatc cgccgcctgg cgcccgcacc cgtggccccg gtggcctcgc
     2461 ccacagaccc caacgaaagg accactccat gaaggccacc atctggcaca accccgcctg
     2521 cggcacctcg cgcaagacgc tggcgatcct gtcggaaacg ccgggcatcg agctgacggt
     2581 cgtggaatat ctgaagaacc cgccctcggc cgaaaagctg gcgcagctgt atcgcgatgc
     2641 cggcctcacg ccgcagcagg gcctgcgcct gcgcggcacc gatgcggcag agcgcggcct
     2701 gcccgaggcc gatgccgcca ccgtgctggc cgccatgacc gcagagccga tactgatcga
     2761 gcggccgctg gtggaaaccg aaaagggcgt ggccctgtgc cgcccgcagg aaaaggtgct
     2821 gacgctgctc tgagcgccag cacggcgatg aggcgcgcct agccccagac gccctcatgg
     2881 agcgcggccg cttcctcttc cagcagcggg cccaccactt ccacctgccg ctggccggcg
     2941 gcaaacacca cccggcaggg cagatcgagc gtggggtttt ccggatgatc gccggtgatc
     3001 tccttcagcc ggctttcggc caggccatag accacccggc ccacccccac ccagtaggcc
     3061 gcgcccgcgc acatcgcgca gggctcggcc gaggtgtaga gcgtggcgcc ggccagcgtc
     3121 gcgcggtcaa acgcgatcga ggcgcgggtc atcagcacgc gttcggcatg gccggtcatg
     3181 tcgtggtcgg gctggaaggc attgccctgc tccatcagga tcgtgccatc cgcgctggcc
     3241 agaatggcgc cgaaagggtg gctgccggcc gccagcgtgg cccgggccac gcggaaagcc
     3301 tcgcgcagga ggcgcgcgtc gtcgggcgcg ggggcgggaa gggtgtcggt catgggaagt
     3361 ctccggggcg agcgcccccg ggcgatggcc tcagcaccac tgctcgcaca tccgcccggt
     3421 gagaggatag cgcgccgcag cgccctgtcc accccggggg gctgcccgcc agcctgtgcg
     3481 caaccgcgcg cctgcccctt gccaagcccg cggcaaaccg ccacacagcg cagcacaccg
     3541 cagtttcatt gctactatcg ggaccttaca gcaagatgac aaccgagctt gcccaatccg
     3601 ccctgtggcc caggctgaaa cggcatgtga agcaggcgat gggcccctgg ggtgttttcc
     3661 tcgaaggctt cttcaaaaat ccggtgatgg ttggctccat cgtgccttcc tcgcgcttca
     3721 ccatcgccaa ggtgctgggg ccggtggatt gggagaacac gaagctgttc gtcgaatatg
     3781 ggccgggggt gggcaccttt tgccgcccgg tgctcagccg gctgcggcgc gatgccatgc
     3841 tgctggtgat cgacaccaac ccgatgttca tcgactatct gaaggccacc gtctccgaca
     3901 gccgcttcgt ggcggtgctg ggatcggccg ccgatgtgga agagatcgtg cgcgcccacg
     3961 gccatgaaaa ggccgattac gtgctctcgg gcctgccctt ttccaccctg ccgccgggca
     4021 ttggcgcggc catcgcttcg gccacgcacc gggtgctgcg ggtgggcggc gcgtttctgg
     4081 tctatcagtt cacgccggcg gccggccatt tcatggcccg cttcttcgcg cggatcgaca
     4141 aggggctgga atggatcaac gtgccgccct gcttcctgtt ctggggctgg aaggacgccg
     4201 agggataggt tttccccccg gcgcgcgcat cgatcagaac agcgccgccg gctgcaccga
     4261 aagctggcgg tgaacggccg ccagcaagcc ggtgagatag acgacaaacg ctgccgccag
     4321 caccgattcc accaccgccg agaccacccg cgcgccatct ggccccagcg ccagcgcggc
     4381 ggccgccccc agcacgctgg tggccgccag catcaccacc accagcgcca ccagcaccag
     4441 cgccagcagc accgccagcc gcccggcatt gcccgcggtg agcgcccagc tgcggcggat
     4501 ggccgccagc ggattggcca ccccatccac cgccaccacc ggcgccagca gcaccagccg
     4561 caccgccaca taggcaaagc cgcccagcac cacgcccagc agcagaccgc cggccgcctg
     4621 ttgctggccg cccagcgcca gcgccacaga agcggccacc gccacggcca tcgccacgcc
     4681 gatctgcgcg gccagatagg gcagcagcgc ccgcacgccc tggccgatcg cctcgcgcac
     4741 ggtggggcgg cgcctgtcgg caaacagcgc cagcatcgcc agcgtgccca ccgtttccag
     4801 cagcaccgtg ggcagcagca tcggcagcac cttcagataa taggcctgca tctggcgcag
     4861 catttcctcg ggcgcggcgc ccgtggcagg ggccaccggc tctggcgcca gcagcgcgaa
     4921 agccagcctt ggcagcagga aaaacacccc tgccagcgcc agcagcacat cgcggttggc
     4981 atagatggca gcgctggcct ggcgccaggc aagggatgag tcgagcttca tcatgggtcc
     5041 tccagaatgc tgcggggctg gccgctggca gggcgcatgt caaggcatgt cggggcatgt
     5101 cggggccggc ggtggcctaa agcggggggc gcaacatgcc tcttgccatg gcgcgcgaat
     5161 ggcgccacct gatcggcgat gactgcgccc agccccgatc cgcaagccct gcccccagcg
     5221 ccggaatggc ggcgcgaggc agcgcccgtg ccctatcgca ccgcgctggc cgagatggag
     5281 gcgcgcaatg ccgcgattgc cgccggcacc gcgcccgagc tgatctggtt gctggagcac
     5341 ctgccggtct acaccgccgg caccagcgcg gcggcggccg aaatgctcga tccgcgcttc
     5401 gaggtggtgg aagccgggcg cggcgggcgc tacacctatc atggcccggg ccagcgggtg
     5461 ggctatgtgc tgctcgatct gaaaacccgc gcgcgcgatg tgcgcggctt tgtccatgcg
     5521 ctggaaggct gggtgatcga cacgctggcc gattttggcg tggccgcctg gcgatcggaa
     5581 gcgggcgttg gcatctggac cgaggatatc gacgggcgcg aggccaagat cggcgcgatt
     5641 ggcgtgcgca tccgccgctg ggtaacgatg cacggctttg ccgtgaacct tgaccccgac
     5701 ctcacccatt tcaccggcat cgtgccctgc ggcattgccg aaaagggcgt gacgagcctg
     5761 gcgcgcctgg gcaagccggt cagcgcggcg cagtgggacg aggcgctgat cgcccgcgcc
     5821 gatgccttcc tggcccggct gcaacagccc tgcccccctt cggagacgcc cgcatgacgt
     5881 ttatccgcct gcccctggcc cgcctgcctt tagcccgctg gctggccggc ctggcgctgg
     5941 ctgccctgcc ggccgcctgc cacaccgccc ccaccaaaag cgacgaccag cgcaccgccg
     6001 gcgggcaagt gctcaaaggc tctgccagcg atggcatgat cccctatgag gcgctgacat
     6061 ccgagccgcc gatggccccg cgcatcgccc cccgcgccgc cgaggccacc tctgccaccg
     6121 cggccgacgc cgccaccgcg caggggaccg atgccgcccc gccgcaagca cccgcgcccg
     6181 cccccggctt cagccagtaa gcgggcgccg ggccgcctgt ttcagcgcgc gcccagatcg
     6241 cctttgccaa cgctggtgct ggccatttcc agcatccggt cgaggctggc cttggcctgc
     6301 agccgcaagc cttcctcgat ctcgatgcgc gggctcaggt cgcgcaggct gacatagagc
     6361 ttttccagcg tgttcagcgc catgtagggg cagatgttgc agttgcagtt gccatcggcc
     6421 ccgggcgcgc cgatgaaggt cttgccgggc agggcgcggg tcatctggtg gatgatatgc
     6481 ggctctgtcg ccacgatcag cgtatcgccg gccatctgct tggcatagtt cagaatgccg
     6541 ctggtgctgc cgacataatc ggcatgatcg acgatgctgg gcgggcattc cgggtgggcg
     6601 gcaatgggcg cgccggggtg ctgcgctttc agcttcagca gttcggtctc gctgaaggcc
     6661 tcgtgcacga tgcacacgcc cggccacagc agcatcggcc ggttgaactt gcgggcaaga
     6721 tagccgccca gatggcgatc ggggccgaag atgatcttct ggtcggccgg gatctgctgg
     6781 aggatggttt ccgcgctcga cgacgtcacg atgatgtcgg acagcgcctt cacctcggtc
     6841 gagcagttga tgtaggtgag cgcgatgtga tcggggtgcg cctcgcggaa ggcccggaac
     6901 ttttcgggcg ggcaggcatc ctcaaggctg cagccggcgt tgagatcggg cagcaccacg
     6961 gtcttttccg ggctgaggat cttggccacc tcggccatga acttcacccc gcaaaaggcg
     7021 atcacctcgg catccgtctc ggccgccttg cggctcagct ccagcgaatc gccaacgaaa
     7081 tcggccagat cctgcagttc gggcttctgg tagtaatgcg ccagaatgat cgcgttgcgc
     7141 tccttgcgca ggcggtcgat ctcggcgcga atatccaggc ccgcaaggct ggcggcggaa
     7201 aaggtcatgc tttgtctcct cctgggccgg tggccgccca ccgatcgccc tgccgccacg
     7261 ccagcgcgcg cgcttccaga tcgatcagat gggccagcac cgaaaggccc gccgcgccca
     7321 ttagcgcagg cgccagcccc ttgtacatcc gcccgacaag atcctgcacg cttgccccgc
     7381 catcggccag atgggtgagg atctgccgct cgcgctggcg gcgatggccg atcatcgagc
     7441 gcacgaactg gcgcggattg tcgatctgcg gcccgtgggc gggataatag acccggtcct
     7501 cgcgcccatg cagcctgtcg aggctgatga gataatcgct catgttgcca tcaggcggcg
     7561 ccaccacgct ggtggaccag cccatcacat gatcgccggt gaacagcgcg ccattggccg
     7621 cgcccgccac ttcccccgcc acttcccccg tcacttccag cgcatagcac aggtgattcg
     7681 aggtgtggcc gggcgttgcc accgcgcgca gggcaaagcc ggggcccgca atcacctcgc
     7741 catcggccag cacggcatcg ggggcatagg aggcatcgaa ggaatcatcg agccttggcc
     7801 catccccctc gatcaccagc ggcgcgcagc cgatcaccgg ggcgccggtg cgcgcggcca
     7861 gcggggcggc ggccggcgaa tgatcgcggt gggtgtgggt gcaggcgatc gccagcaccg
     7921 gcgcgccgcc gatcgctgcc accagcgcat cgagatgggc ggcatcggca gggcccggat
     7981 cgatcaccac cacgccatcg ccatcgccga ccagataggt ctgcgtgccg gtgaaggtgt
     8041 aggggctggg gttggtggcc agcacgcggc gcaccagcgg ctccagctgt tcggcggcac
     8101 ctgtgggcca gggatggggg ggcaggctca tgccgcccat atggggatgg caggcgcttc
     8161 tgtcgagccg gcggatcgcc tggcggccat atgggcgggc tggtttttgt gcgaggggag
     8221 agccttgcca gtcaggcgac agagcggcgc aggcgttcct gcagggcgcc cggcccgccc
     8281 tcgcgctctg catcggggca gcgccaggcc tcgtccagcc gcagcagccg ggcatagaaa
     8341 tcgcacgaga gcgccaccag cacgcgcact tcctcgggca gcagcgccag ccggtcgggc
     8401 tctgccggct ggtcggccgg caggcggccc tggcggcgca gctggctttc cgacagttcg
     8461 ccgcgctgga aggcccggtg gttgagcagc caggccagcg catgcatcac ccgcgtggtg
     8521 gtgcgcagcc cctcgcagga aagggcaatg cggccggcat cttcaggccc gcccgccacc
     8581 ggcgctgcca gcatcgcatc cagccggccg gccaggcgaa aggccgcgcg cacctcgtca
     8641 gacagcacca gcgcctcggc atagagcgtc tcgatgatgc gcgggttgat atcgctggtc
     8701 tggctcatgc ggcaacggat aaaaccgccg cgccgcagcg gcaatcgcca tcaccccggc
     8761 ccgcttcccg tttcgggaca ggcgtgcaga tccggcacgc cccacctctc tcaggcgatg
     8821 atatcgggga tgagatagtc ctcgatgatg gcgatctggt ccttcagctt cagcttgcgc
     8881 ttcttcagcc gggcgatctg caattggtcc accccgccgg tcacattcac cgccacgctc
     8941 agcgcgtcga tcgcggcatc aaggtcgcgg tgctccaccc gcagggcttc gaggcgcttg
     9001 cgcatttcct cttcggtcat gccgcctctc ccgggataca aaacggcatt ttagggtgaa
     9061 tttgggccac cggattcgct tttcgtgctg cccgtcgggc tgtgggtatg atctgttcgt
     9121 cgcggcgtgg cgcggttcgt ctcaaattcg ccgcgagccg cttcgcaaga taggctcaat
     9181 atggttgaat gacaatcgcg ccggttgagg caagtgagtc gcggctttgt cataaaagcc
     9241 ggggctggtg tctttttccg gcgtgccgcg ggcagggcca ttgcccggca tagcaacaag
     9301 gggatcaacc gccccgtttc tgctccggcc tgccgggccg gcctgtcagt ttgatctgag
     9361 cctgtcagct tgatctgaga aggccggtgt ggtcgtccgc cgggggcaaa ggcgcacgag
     9421 aaggcttgcc cgccatccgg gccggccttg catcgccggg cagatcggcc ccttcccggg
     9481 ccagcgcggg catgaagcca acccaacctc aaaccccatt tgcacggccg cacagaacat
     9541 caagccaggc atgcgcctgc gccaaggccg ctgcatcgca aggagaacgt catggaagct
     9601 tcgcacacca gcgccctgct gaacaagcac gcaggtcttg agcggatgat acagcaggaa
     9661 ctgaaccgcc ccctgcccga tctcgccatg gtgcaatcgc tgaaacgacg caaattgcga
     9721 atcaaggacg cgctcacgca gaattgagca cccggccgca ggttcaggcc gcagggcggc
     9781 tggcgcatcc ccgcgatgcg ccagcccgat ccggacagcg gccggcccct gtcaccggcc
     9841 cggctctgcc accagcccgc caccgcctgt cgccgcaaag catcagcctg tcgccccaaa
     9901 gcatctgcgt tgcgccagcg ccgtttccaa agccgccgct ttcggctagg ccatcggcca
     9961 tgaccagcgc acctgtcggc cccacctccc cttccagcgc cgtaaccgcg gcgcgcctca
    10021 tcctcacccg cctgcacgat gtgatggcct cgcgctataa cgcccaggcc aagctcaaca
    10081 ccgtggtgga agtgatcggc gaaagcctca acagcgaggt ctgctcgatc tacctgctgc
    10141 gcgagggcat gctcgaactg ttcgccaccc gcggcctggc gcaggaagcg gtgcacgtca
    10201 cgcgcatggc cgtgggcgaa ggcctcgtcg gctccattgc cgagcatagc gagacgctga
    10261 acctggcgga agcggccgcc caccccgact tttcctatcg cccggaaacg ggggaagaca
    10321 aataccactc ctttgccggc gtgccgatcg tgcggcgcca gcgctcggtg ggggtgctgt
    10381 gcgtgcagca tgtcgatccg cgccgctacg aggaagtgga gatcgaggcg ctgcagacgg
    10441 tggcgatggt gctggccgag ctgatcgcca atgccgggct gatcgacgag gaagacacgc
    10501 tgggcggcgc cggcgcgcaa accggccccg agcagctgcg cggcctgacg ctggtgaagg
    10561 ggctaggcgc gggggtggct gtttatcacc agccgcgcgt gcagatcgag catgtggtgg
    10621 ccgaggacat cgaggccgag cgccagcgtg tctacctcgc cttcgacaag atgcgcgagc
    10681 agatcgagcg catggccagc caggccgaat tcggcgtggg cggcgagcat gaggaagtgc
    10741 tcgagaccta tcgcatgttc gcctatgacg agggctgggg ccggcgcatc aacgaggcga
    10801 tcgattccgg cctcaccgcc gaggcggcga tcgagcgcgt gcagcagcgc acccgcatgc
    10861 gcatgcgcca gatcgacgat ccgctgctgg ccgaccgcat gcacgatctg gaggatctct
    10921 ccaaccggct gctgcgcatt gtctcgggcc agctgggcac ggcggccagc atgggcctca
    10981 ggcaagatgc catcctcatc gcccgcaacc tcggcccggc cgaactgctg gaatatgaca
    11041 agcgccggct gaagggcgtg gtgctggagg aaggctcgct cacggcgcat gtggtgatcg
    11101 tggcgcgggc catgggcatt ccggtgctgg ggcgggtgaa gggcctgcgc ggcatggtgc
    11161 gcgagggcga tccgctgctg ctcgatgccg acaaggcggt ggtgttcgtg cggcccacgc
    11221 gcgatatcgc catcgccttc gacgaacgct tcgccaagac gcgcgagcgc caggctggct
    11281 atgccgcgct gcgcgatgtg gtgccgatga ccaaggatgg cacgcgcatc accgtgatgg
    11341 tgaatgcggg cctgcgcgag gacatgccca acctgcagct gactggcgct gatggcgtgg
    11401 gcctgttccg caccgagttc cagttcctgg tttccgccac cctgcccagc cgcgagcggc
    11461 agacgcgcct ttaccgcgag gtgatggatg tggccggcag caagccggtg gtgttccgca
    11521 cggtggatat tggcggcgac aaggtgctgc cctatctgcg ccacgatggc gatggcccgg
    11581 ccgaggaaaa cccggcgatg ggctggcgcg cgctgcgcgt ggcgctggag cgcgacgggc
    11641 tgctgaaggt gcaggcgcgc gcgctgctgg aagcctcggc cgggcgcacg ctgcatgtga
    11701 tgttcccgat gatttccgag ccgtgggaat tcgatgccgc caaggcggtg ttcgatggcc
    11761 agctggccca tctgcgcgcg ctgaagaagc cgctgcccga agcggtgaaa tttggcgcca
    11821 tgctggaagt gccggcgctg gccgacatgc tggatgtgct gtgcccgcgc ctgtcgttcc
    11881 tgtcgatcgg cacgaatgac ctgacgcagt tcctgtttgc cgccgatcgc gcccacccca
    11941 agctggccga gcgctatgac tggctgagcc cggcgatcct gcgcttcatc cgccgcatcg
    12001 cgctggcctg ccaggcgcat aatgtgccag ttaccgtgtg cggcgagatg ggcgggcgcc
    12061 agctggaggc attggcgctg ctgggcgtgg gcatccgtcg cctgtcgatc acgccggcag
    12121 cggtggggcc gatcaaggcg atgctgcatc aggtggatct gcccgccatc ggcgcggcga
    12181 tggaaggctg gctggcccag ccgccgcgcg atctgcgcgc cgaactggtg gcctgggcgc
    12241 aaggccaggg catcgtgttc gactgacgtg ttcaactgac gcgtttggca gaaccgcgcg
    12301 ccccaatatg gtgaacgaat ttgtttgcat tcgctcgctt ttcattaggg caatcgcaga
    12361 gtgcatggta gagccggcga ccatgaccgc aagcggtcat aaagggttgc caaagaccgg
    12421 aaacgagggc cacacggctc cctttgcgcc cctttccccg ggcgcgacgt gacggacatt
    12481 cgagagtgtt atgggcgcgc aagacagtgt gttagacgat acccccgcac atacgggcga
    12541 gatgctgcgg cgcgcgcgcg aggcggcggg ccttgatctg gccgatgtgt ccgcccgcac
    12601 ccgcatcccc gaacggcacc tgcgcgcgct ggaagaggcc gactataccg ccctgcccca
    12661 ccgcacctat gcgctgggct ttgcccgcac ctatgccggc gtgctggggc tgaatgcgga
    12721 agatgtgatc gcctcgctgc ggcgcgacta tcgcggcgcc atgcccgagc ccgagcccac
    12781 cccgcccgcc ttcacccccg gcgatccggc ccgcgtgccc agcgcccgct tcgccttcat
    12841 cgccggcctg gcggtgctgc tgctgggcgt gggcggcttt gcctattggc agggctttgg
    12901 cagcgatgtc agcctgccct cgctgctgcc cgacaatccg ccgcccccgc cgccggccgc
    12961 caaacccgcc gccgcgcccg cccctgcccc cagcgcccag cccacagggc cggtggtgtt
    13021 cacagcgcaa gccaagggca tctgggtgaa gttctacgat ggcgatgcca gccatgtgct
    13081 gctcgaaaag cagctggccg agggcgaaag cttcaccctg ccggcagagg ccaaaaaccc
    13141 gatgatctgg accggccgcc ccgaggcgct ggcgatcacc atcggcggcc agccccagcc
    13201 caagctggcc gacgcgcaaa agatcatgcg cgatgtgccg gtgagcgcca ccgcgctgct
    13261 ggcccgccag gccgcgcccg ctgcccccgc ccaaccggcg atggggccga cgatgggccc
    13321 cgcaatggcg ccccaacccg gcatggcccc tatgcccggc atgatgacgc cttccgcgcc
    13381 gacctcggcc gcagcgctgc cggcaccgcg cccgcaccgg ccccacccgc accccaccgc
    13441 cagcagtggc gatgcggctg ccccggccgc cgccgcgccg gcccctgtgg cagcgccggc
    13501 cccggcccag ccatccaccg tttcgcaata ggcccaactc gacagcgggc gcgcgctgag
    13561 gcaatcaggc gcatcgaacc gccaaccggg gaggcgcgcc acggccgccg ggctttgccg
    13621 cccggccacc gccccgccgc cccagaccgc acaggagcac gcagatcatg acaagcacac
    13681 cgcgccgggc caggggcctg cttgccgccg ggccgctgat ggcgctggcc ctcatgctgg
    13741 ccggcacgct ggtgaccacc gccacgcccg cccatgccga agacagcagc gaccaggaag
    13801 tgcgcctgcg caagctggaa gccgaagtgc gcgccctgca gcgccaggtg ttcccgggcg
    13861 gtgatcgcaa gttcttcccg caggatgcaa cgccgcaaac cggccctgcc gcagcgcccc
    13921 agcccggcat gccggccacc acgccgatca ccgacctgct ctcgcgcatg gacgcgatcg
    13981 aggcgcagaa cacccgcctc acctcgcaga acgaggaact ggccaaccgc gtgcgcattc
    14041 tggaagccgc ccgcgcccag gccctgtcgg ccgaagcgcc gccgccggcc ccgccgcccg
    14101 ctgtcggcta tgcgcccagt tatggcccgg cggcaacgcc ggcgcccgcc gccgcgcccg
    14161 cgccccggcc tgccgcccgc ccggcccctg tcgatggcga tctgggcggc cagcgcgcgc
    14221 cggccgccgg ccccatcgcc cgccccagcg cccagcgcct ggccggcgtg caggcgatca
    14281 tcaagccgca gacgggcgat gccggcgaag atgaatatac ctatggcttc aagctgtggg
    14341 aggcgaagtt ctatcccgag gctgagcagc agctgaagct cttcctcgac cattacccgc
    14401 gccaccgccg cagcagctat gcccgcaacc tgctgggccg cgcctatctc gacgaaggca
    14461 agccgcgcga ggcagccccc tggttcctgc agaactatca gggcgacaag aagggcgatc
    14521 gcgccgccga cagcctgctc tatctcgccg aaacgatggt gaagatgaag gacaccaacc
    14581 gcgcctgcat cgcgctggcc gaatttgccg acacctacaa gaccgaggcc gccggccgcc
    14641 tgcagggcca gtataacagc accaccgccc tggcgaagtg caactgagcg acgccggccc
    14701 ggatgaagct ggcccggatg aagcgggccg gctgacggca gagctggccc ggctgtggcc
    14761 ggaaggggcg cgccagggcc ggctgggcct ggccgtctct ggcgggccgg atagccttgc
    14821 gctgctgctg ctggcccatg ccgcctggcc cggccgcgtg gccgccgcca cggtggacca
    14881 tggtctgcgc cccgaaagcg ccggcgaggc ggccgaggtg gcgcgcatct gcgccgggct
    14941 gggcgtggcc catgccacgc tgccggtctc cataggcccc ggcaatatcc agaccgaggc
    15001 gcgcaccgcc cgctatgccg cgctggcgca atggatggcg gccgaagggc tggcggcact
    15061 ggccaccgcc caccatgccg acgatcaggc cgaaacgctg ctgctgcggc tgaaccgggc
    15121 cagcggcgtg gcggggctgg cgggcgtgcg cgcgcgggcg ctggtgccgg gcagccggct
    15181 gctgctgctg cgcccactgc tgggttggcg gcggg
//
LOCUS       DASRYE010000144         6723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_114403,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000144 DASRYE010000000
VERSION     DASRYE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114403"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFF98_07775"
     CDS             complement(<1..358)
                     /locus_tag="VFF98_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HZU63566.1"
                     /translation="MELDLADVFAGAALGGNPLAVVRGGEGLTGAAMQALTRWLGFSE
                     TTFLLPPTDPAADYALRIFTPAAELPFAGHPTLGSAMAWLAAGGTPRCASVVMQQCGV
                     GLVPVRMDAHNPARLAF"
     gene            complement(373..834)
                     /locus_tag="VFF98_07780"
     CDS             complement(373..834)
                     /locus_tag="VFF98_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU63567.1"
                     /translation="MPIDSFDRAIIAALQADARLPVSAIAEQVALSATPVSRRLKRLE
                     EEGYIQGYAPLLNLRKLGFELNAYVLVNLQIRSDENIDLFEREVIANPYVVSCHAVTG
                     EMDYLLHVVARNVDHLNQSTLKALVRIPGVRDVKSIIALEDVKPVQPVPLD"
     gene            942..2387
                     /gene="astD"
                     /locus_tag="VFF98_07785"
     CDS             942..2387
                     /gene="astD"
                     /locus_tag="VFF98_07785"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HZU63568.1"
                     /translation="MTNPAPASASALLRSIDPASGALVWEGEVADAAAVGQAVAAARG
                     AFPAWAALAQDARTALVRGFAGVLQARSGMLAETISRETGKPLWEARGEVASMIGKVE
                     LSIAGQAARAGEQHTAAPFGAAVLRHRPQGVVAVFGPYNFPGHLPNGHIVPALLAGNA
                     VVFKPSELTPATGAAMAGAWAAAGLPEGVLQLVQGGPATGAALADADIDALLFTGSAA
                     TGAVLRQQCAQRPRVLLALELGGNNPLVAWDGDVAETAALVVQSAYLTSGQRCSCARR
                     LIVPDNRFGTELIEAVADLAASLRIGAWQETPEPFMGPLVSAAAASRAEAQVAQLVSL
                     GARPTRPLARPQGRSAAFLTPALLDVTGLAVPDEEIFAPVLQWRRVGDFEAALEAANN
                     SRFGLAAALVSEDEALWQRFAREVRCGVVNRNRPTTGASGAMPFGGLGESGNHRPSAF
                     YAADYCAQPVASFEAPTAAGHRAALAGMVRA"
     gene            2384..3586
                     /locus_tag="VFF98_07790"
     CDS             2384..3586
                     /locus_tag="VFF98_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZU63569.1"
                     /translation="MNALSPAERQLVAAIDPAPILAHVLDWAAINSGTGHLAGLATMA
                     ARLADAFAVLPGDAQLVAPAPVAAIGADGAAHPRAHGAHLLLRVRPDAPRRLLLTGHM
                     DTVFPADHPFQQCAWLDEDTLRGPGTADMKGGIALMLAALVAFEQGRPALGYDVLLNS
                     DEETGSLSSAGLIGQCARGKLAALTFEPALPDGAMAGGRPGSGNFALVIRGRAAHAGR
                     NPAEGRNAVLAAADLALRLAAARRPGLAINVARIEGGGPDNQVPDLAILRCNLRPARA
                     ADAAEAEALMATAIAAVAAAHEVSIHRHGHFTRPPREIAARDRPLRALVGQAAGDLGE
                     ALHWADTGGVCDGNNIAACAVPVVDTMGARGGAIHSPQEFLQVSSLAARARLAALVLH
                     RLDGEGRA"
     gene            3598..4635
                     /locus_tag="VFF98_07795"
     CDS             3598..4635
                     /locus_tag="VFF98_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HZU63570.1"
                     /translation="MPLVVRAVRTGDVPALYRMAQQTGAGFTNLPADLPALEAKVARA
                     LACFARPEEAPADDLYLFVLEDPATGALCGTCQIFARIGSQWPFYSYRLGALTQFSKE
                     LGRAFRAETLNLCTDLDGASEVGGLFLEAGARAAGAGRLLARSRYLFMRRHRARFASR
                     TIAELRGALDEAGRSPFWEGLAGRFFGMPFDEADGFNALHGNQFIADLMPKHPIYTSM
                     LPEAARASIGQPHRNGEAAMRLLQQEGFRFDCYVDIFDGGPTMVVDTDRIATLRGARL
                     ARVAALADAGRPEGAPGALVATGALGDFRCCIGAVAGEGEAVVLNAAAARALGVGPGD
                     AILYTGLLKAE"
     gene            4640..5914
                     /locus_tag="VFF98_07800"
     CDS             4640..5914
                     /locus_tag="VFF98_07800"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HZU63571.1"
                     /translation="MLREINFDGIVGPSHNYAGLSLGNLAATRHAGQVAFPREAALQG
                     LAKMRANLDLGLVQGLFLPHDRPHESWLAAMGTSLARAGQALRANALSASAMWAANAA
                     TVSPAPDTADGRCHLTVANLLTMPHRSHEWPQTLAQLRLAFADAAHFAVHGPVPPPFG
                     DEGAANHMRLAADHGAPGIEVFVYGEGGGPFPARQRREASEVVARRHGLDPARVLFAR
                     QSAEAIAAGAFHNDVVAVANGPVLFTHEQAFADRHALYDGLRARLPGVVIEEVPAAAI
                     SLAEAISTYLFNAQLVSLAEGGPGEGGMALILPGEARAHAGVWGWLEGLVAKGGPIRR
                     LLPVDVRQSMANGGGPACLRLRVVADPARVDPRFLVNHARIDAIARIVQGYWPEQIAP
                     DDLASPALWQACRKARARLLEGLDLGALLAAG"
     gene            complement(6122..>6723)
                     /locus_tag="VFF98_07805"
     CDS             complement(6122..>6723)
                     /locus_tag="VFF98_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZU63572.1"
                     /translation="LAEALPIAIALARAVAALHRLEIVHRDIKPENVLLPQEGGLKLI
                     DLGVARLPRVDDFAVDEIPGTPGYLAPEMFEGEAGSAATDQFALGVTLWRLFAGSWPY
                     GQAEAFARPRFRSPTPPSRSRPEIPGWLDAVLQRAVAIDPADRFGDMIEMLRALEGGA
                     AVERVAPRRAPLIERHPVAFWQAVSALLFVALIVALVRR"
BASE COUNT          919 a         2304 c         2442 g         1058 t
ORIGIN      
        1 ggaaggccag gcgggcgggg ttgtgcgcat ccatgcgcac gggcaccagc cccacgccgc
       61 attgctgcat caccacgctg gcgcagcgcg gtgttccgcc cgctgccagc caggccatgg
      121 cagaacccag cgtcgggtgg ccggcaaagg gcagctcggc cgcaggcgtg aagatccgca
      181 aggcataatc ggccgccgga tcggtgggcg gcagcaggaa ggtggtttcc gaaaagccca
      241 gccagcgggt gagggcctgc atcgccgcgc ccgtcagccc ctcgccgccg cgcaccaccg
      301 ccagcggatt gccgccgagc gctgcgcctg caaacacatc ggccagatcc agttccatcc
      361 ttgccatttc cttcaatcca gcggcaccgg ctgcaccggc ttcacatctt ccagcgcgat
      421 gatcgacttc acatcgcgca cgccgggaat acgcaccagc gccttcagcg tcgactggtt
      481 gagatgatcg acattgcgcg ccaccacatg cagcagataa tccatctcgc cggtcaccgc
      541 atggcacgag acgacatagg ggttggcgat cacctcgcgc tcgaacaggt cgatgttctc
      601 gtcgctgcgg atctgcaggt tcaccagcac ataggcgttc agctcgaagc ccagcttgcg
      661 caggttgagg aggggggcat agccctggat atagccttcc tcctccagcc gcttcagccg
      721 ccggctgact ggcgtggccg acagcgccac ctgttcggca atggccgaga cgggcaggcg
      781 cgcatcggcc tgtagcgcgg cgatgatcgc gcggtcgaag gagtcgatgg gcatggattc
      841 cctccgtatg gccactgaat tggtggatac ctctcatagc agccggtttg gcggcaagga
      901 tagcacggat ataccgcctg tttggtgata ggctgcatgc catgaccaac cctgcccccg
      961 cctccgcctc cgccctgttg cgctccatcg atccggcgag cggcgcgctg gtgtgggaag
     1021 gagaggtggc cgatgcggcg gcggtgggcc aggcagtggc ggccgcgcgc ggtgctttcc
     1081 cggcctgggc ggcgctggcg caggacgcgc gcaccgccct tgtgcgcggc tttgccggcg
     1141 tgttgcaggc gcgcagcggg atgctggccg agacgatctc gcgtgagacc ggcaagccct
     1201 tgtgggaagc gcggggcgaa gtggcctcga tgatcggcaa agtggaactg tccatcgccg
     1261 ggcaggccgc ccgggcgggt gagcagcaca ccgccgcccc ctttggcgcg gcggtgctgc
     1321 gccatcgccc gcagggggtg gtggccgtgt tcggccccta taatttcccc ggccatttgc
     1381 ccaatggcca tatcgtgccg gccctgctgg cgggcaatgc ggtggtgttc aagccatcgg
     1441 aactgacgcc ggcgacgggc gctgccatgg ccggggcctg ggcggccgcg ggcctgccgg
     1501 aaggcgtgtt gcaactggtg cagggcgggc cggccacggg cgcggcgctg gccgatgccg
     1561 atatcgatgc gctgctgttc accggctctg ccgccaccgg cgccgtgctg cgccagcaat
     1621 gcgcgcagcg cccccgggtg ctcctggcgc tggagctggg cggcaacaat ccgctggtgg
     1681 cctgggatgg cgatgtggcg gaaaccgcgg cgctggtggt gcaatcggcc tatctcacca
     1741 gcggccagcg ctgcagctgc gcgcgccgcc tgatcgtgcc cgacaaccgc tttggcacag
     1801 agctgatcga ggcagtagcc gatctggccg ccagcttgcg gatcggcgcc tggcaggaaa
     1861 cgccagagcc cttcatgggc ccgctggttt cggcagcggc ggccagccgg gccgaggcgc
     1921 aggtggccca gctggtgtcg ctgggcgcgc ggccaactcg gccgttggca aggccgcaag
     1981 ggcgcagtgc agccttcctc accccggcgc tgctggatgt gacgggcctt gccgtgcccg
     2041 acgaggagat cttcgccccc gtgctgcaat ggcgccgggt gggcgatttc gaggccgcgc
     2101 tggaggcggc caacaacagc cgcttcggcc ttgccgccgc gctggtgagc gaggacgagg
     2161 ctttgtggca gcgctttgcg cgagaggtgc gctgcggcgt cgtcaaccgc aaccggccga
     2221 ctaccggcgc atcgggggcg atgccctttg gcggcctggg cgaatccggc aaccaccggc
     2281 ccagcgcctt ttatgccgcc gattattgcg cccagccggt ggccagtttc gaggcgccca
     2341 cggccgccgg ccatcgcgcc gcgcttgctg gcatggtgcg ggcatgaacg cgctctcgcc
     2401 cgccgaaagg cagctggtgg cggccatcga tccggcgccc atcctggccc atgtgctgga
     2461 ttgggcggcg atcaacagcg gcaccggcca tctggcgggg ctggccacca tggccgcccg
     2521 gctggccgat gcctttgccg tgctgccggg cgatgcgcag ctggtggcgc cggcgccggt
     2581 ggcggcgatc ggtgccgatg gcgctgccca cccgcgcgcg catggagcgc atctgttgct
     2641 gcgcgtgcgc cccgatgcgc cgcgccggct gttgctgacc ggccatatgg acaccgtctt
     2701 tcccgccgat caccccttcc agcagtgcgc gtggctggac gaggacacat tgcgcggccc
     2761 cggcactgcc gacatgaagg gcggcatcgc gctgatgctg gccgctctgg tggcgttcga
     2821 gcaaggccgg ccggcgctgg gctatgacgt gctgctcaac agcgatgagg agaccggctc
     2881 gctctcctct gccgggctga tcgggcaatg cgcgcgcggc aagctggccg cgctcacctt
     2941 cgagccggcg ctgcccgatg gcgcgatggc cggcggccgg ccgggatcgg gcaatttcgc
     3001 gctggtgatc cgcgggcgcg ccgcccatgc cggccgcaac ccggcagagg ggcgcaatgc
     3061 cgtgctggcc gcggccgatc tggcgctgcg gctggcggcc gcgcgccggc ccgggctggc
     3121 gatcaatgtg gcgcgcatag agggcggcgg gccagacaat caggtgccgg atctggccat
     3181 tttgcgctgc aacctgcgcc ccgcccgcgc cgccgatgcg gcagaggccg aggcgctgat
     3241 ggccacggcc atcgccgctg tggccgccgc gcatgaggtc tccatccacc gccacggcca
     3301 tttcacccgc cccccgcgtg agatcgctgc ccgcgatcgc cccttgcgcg cgctggtggg
     3361 gcaggccgcc ggcgatctgg gcgaggcgct gcactgggcc gatacgggcg gcgtgtgcga
     3421 tggcaacaat atcgccgcct gcgcggtgcc ggtggtggat acgatggggg ccaggggcgg
     3481 cgctatccat tcgccgcagg aattcctgca ggtgtcgtcg ctggcggcgc gggcgcggct
     3541 ggcagcgctg gtgctgcacc ggctggatgg ggaggggcgg gcatgagcgc gcccgctttg
     3601 ccgctggtgg tgcgcgcggt gcgcacaggc gatgtgccag cgctctatcg catggcgcag
     3661 cagaccggcg ccggcttcac caacctgccc gccgatctgc ccgcgctgga agccaaggtg
     3721 gcgcgcgcgc tggcctgctt tgcccggcca gaggaagcgc cggccgatga tctgtatctc
     3781 ttcgtgctgg aagatccggc cacgggcgcc ctgtgcggca cctgccagat ctttgcccgc
     3841 atcggcagcc agtggccgtt ctacagctat cggctgggcg cgctcaccca gttcagcaag
     3901 gagctgggcc gcgccttccg ggccgaaacg ctgaacctgt gcaccgatct cgatggcgcc
     3961 agcgaagtgg gcgggctgtt tctggaagcc ggcgccaggg cggcgggcgc gggcaggctg
     4021 ctggcgcgca gccgctacct cttcatgcgc cggcaccggg cgcgctttgc ctcgcgcacc
     4081 attgccgagc tgcgcggcgc gctcgacgag gcgggccgct cgcccttctg ggaggggctg
     4141 gcggggcgct tcttcggcat gccgttcgac gaggccgatg gcttcaatgc cctgcacggc
     4201 aaccagttca tcgccgatct catgcccaag cacccgatct acacctcgat gctgcccgag
     4261 gcggcgcggg ccagcattgg ccagccccat cgcaatggcg aggcggcgat gcgcctgttg
     4321 cagcaggagg gcttccgctt cgactgttat gtcgatattt tcgatggcgg cccgacgatg
     4381 gtggtcgaca ccgaccgcat cgccacgctg cgcggggcgc gcctggcccg ggtggcggcg
     4441 ctggccgatg cgggcaggcc ggaaggggcg ccaggggcgc tggtggccac tggcgcgctg
     4501 ggggattttc gctgctgcat cggcgctgtc gccggcgagg gcgaggcggt ggtgctgaac
     4561 gcggcggcgg cccgggcgct gggcgtgggg ccgggcgatg ccatcttgta cacaggcctg
     4621 ttgaaggcgg agtgaagcca tgctgcggga aatcaacttt gatggcattg ttggccccag
     4681 ccacaattac gccgggctca gccttggcaa tctggccgcc acgcgccacg ccgggcaagt
     4741 ggccttcccg cgtgaggcgg cgctgcaggg gctggcgaag atgcgggcca acctcgatct
     4801 ggggctggtg cagggccttt tcctgccgca tgaccggccc cacgagagct ggctggcggc
     4861 catgggcacc agccttgccc gggccgggca ggccttgcgg gccaatgcgc tgtcggcctc
     4921 ggcgatgtgg gcggccaatg ccgccaccgt ctcgcccgcg cccgacaccg ccgatggccg
     4981 ctgccacctc accgttgcca acctgctgac gatgccgcac cgcagccatg aatggccgca
     5041 gacgctggcg caattgcggc tggcctttgc cgatgccgcg cattttgccg tgcatggccc
     5101 ggtgccgccg ccctttggcg acgagggcgc ggccaatcac atgcggctgg ccgcagacca
     5161 tggcgcgccg ggcatcgagg tgttcgtcta tggcgagggc ggcggcccgt ttcccgcccg
     5221 ccagcgccgc gaggcgagcg aggtggtggc gcgccgccat gggctggatc cggcccgggt
     5281 gctgttcgcc cggcaatcgg ccgaggcgat tgccgcaggg gcctttcaca atgatgtggt
     5341 ggccgtggcc aacgggccgg tgctgttcac ccatgaacag gcctttgccg accgccacgc
     5401 cctttatgac gggctgcgcg cccgcctgcc cggggtggtg atcgaggaag tgccggccgc
     5461 cgccatcagc ctggcagagg ccatcagcac ctatctgttc aacgcccagc tggttagtct
     5521 ggcggaaggc ggccctggag aaggcggaat ggcgttgatc ctgccgggcg aggcgcgcgc
     5581 ccatgccggc gtgtggggct ggctggaagg gctggtggcc aagggcgggc cgatccgccg
     5641 gttgctgccg gtggatgtgc ggcaatccat ggccaatggc ggcgggcctg cctgcctgcg
     5701 cctccgggtg gtggcggatc cggcgcgggt ggatccgcgc tttctggtga accatgcgcg
     5761 gatcgatgcc atcgcgcgga tcgtgcaggg gtattggccc gaacagatcg ccccggatga
     5821 tctggccagc ccggcgctgt ggcaggcctg ccgcaaggcc cgcgcgcggc tgctggaagg
     5881 gctggatctg ggcgccttgc tggccgccgg ctgaggcgct gcgggccggt tgcatttcgc
     5941 acttgcatca tgaaccgatt cgcggcattt ttgtgcatgc agcccgcaaa ggcgtgggct
     6001 gcccggcgtt cttccgctgc atagcggcat caccgccccc gattttcatc tgtcacggca
     6061 atggcgccgc cctgttgaag gagctgccat gccttgtgcc tttttccccc cgcctatccc
     6121 gctagcgccg caccagcgcg acgatcagcg ccacgaacag cagcgccgac actgcctgcc
     6181 agaaggccac cgggtgccgc tcgatcagcg gcgcgcggcg cggggccacg cgttccacgg
     6241 ccgcgccccc ttccagcgcg cgcagcatct cgatcatgtc gccaaagcgg tcggccgggt
     6301 cgatagccac ggcgcgctgc agcacggcat ccagccagcc ggggatctcg ggccggctgc
     6361 gcgagggcgg cgtggggctg cgaaagcgcg ggcgggcaaa ggcttcggcc tggccatagg
     6421 gccagctgcc ggcaaacagg cgccacagcg taacccccag cgcgaactgg tccgtcgcgg
     6481 cactgccggc ctcgccctcg aacatttccg gggcgagata gccgggcgtg ccggggatct
     6541 cgtccaccgc gaaatcatcc acccgcggca ggcgcgccac gcccagatcg atcagcttca
     6601 gcccgccttc ctgcggcagc agcacatttt caggcttgat gtcgcggtga acgatctcca
     6661 gccggtgcag cgctgccacc gcgcgggcca gggcaatggc gatcggcagc gcttctgcca
     6721 gcg
//
LOCUS       DASRYE010000145         4433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_8241,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000145 DASRYE010000000
VERSION     DASRYE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8241"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..738
                     /locus_tag="VFF98_07810"
     CDS             304..738
                     /locus_tag="VFF98_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZU63573.1"
                     /translation="MSYSFEQHKIIGAIEADDDYWRHLEEGVLKVPRCAGCHTWTWPA
                     HFRCGTCGSWTFDWVEVAAQGLIFSHTRTHYAFDRVLERREDVPYVTLVVELPHAGGI
                     RLIGVLQGSDEGLAIGAQVTGSIDPPSAKSKHYPALRWQLAR"
     gene            791..1948
                     /locus_tag="VFF98_07815"
     CDS             791..1948
                     /locus_tag="VFF98_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZU63574.1"
                     /translation="MRGATAIVGIGQTPYYKRGTSPEPELKLALRAITQAAEDAGIDV
                     RDIDGFISYASERSDPAKLMPALGTRACRFAALVWIHGGALAGTLNIAAGAILSGQAE
                     VVAIYRSMAEGNSQRLRVAVAQNDTSSQYLVNGLDSPAQILALRAQRLMEHHGVPRSA
                     LFAAVQASYYHARANPMAYGRNTEMTREIYDAARPIADPYGLFDCSRENDGAACVLVV
                     SADRAKDLRQPPAYVLSAPMGTMEGGGALEENWRPYYATAGQRELAERMWQEAGYGPG
                     DVDVAQIYMNMNAMGIGSMIDHGFFSFEEAGTFCTFENLIAPSGRLPINTSGGDLAEG
                     FIHGMGLIPEAVRQIRGTSTNQVPGAKLSLFTGGPGDLMSSTALLGSADTL"
     gene            1992..3506
                     /locus_tag="VFF98_07820"
     CDS             1992..3506
                     /locus_tag="VFF98_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZU63575.1"
                     /translation="MAFTVSRALKWWAWDKPDRIALNYEGEALTYAQLYGWALRVADW
                     LRTQGIQPGERVMMVGENTLDYAVLLVGTVLAGGICAPITFRSSERELRRSLELLSPR
                     LVVVDRDREEIASLALGGEGKGRLVLMDSLRALRSAPEPAPAHEARPEDPMFIIGTSG
                     STGTPKGVVYTQGSTMVYAAEFAIMEPATGNGGSVLSAGPYSSASGTLLLFQFLSVGA
                     TIFAENKFTPERALRMLRDERITTFLASTIFFERIAALPEFPAADLSSIKFAQISGAR
                     VSTALIARFQAKGVVLRQAYGCTEAGGAWAARDETALVDPEKCGPGALFTEYAIARPD
                     GTRAAPGEAGEILIRSPALAAGYWHDAEQTARTFRAGWLHTGDRGVQDERGNLTFIDR
                     IKDIIISGGLNISAMEVESAIAEIDGVVEVVVLAARDAEFGETPLAVVHGDLGRIDAR
                     QILQHCRQALATYKVPRYIALEERPLPRLPSGKISKVELREKYKDGPAFLERVR"
     gene            complement(3551..4111)
                     /locus_tag="VFF98_07825"
     CDS             complement(3551..4111)
                     /locus_tag="VFF98_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZU63576.1"
                     /translation="MRLGVVSDIHGQPQALARALELMGPIDRLVCLGDAINQTRFCNE
                     TVAMLRAQAALTIRGNHEDLFFAGGPTGADVDPGLAGWLAACPQRLDIALGGRRLTVV
                     HSTPWPSGHAYVPGHHRDFHRFAVAGADVVLYGHTHQPLVRWLGPVLVVNPGSVGEGR
                     PTPQGFRRSCALVDLAGPAARIIELD"
     gene            complement(4118..>4433)
                     /locus_tag="VFF98_07830"
     CDS             complement(4118..>4433)
                     /locus_tag="VFF98_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63577.1"
                     /translation="RPADIAASGRAFGFAAALLPAAEGAGTASAQIEQALVWPVLAEA
                     WALIDEGVALRAADVDFAMVRAGLWPAWKGGPAYMAAEIGRAALAPWRATDAAPATGV
                     DA"
BASE COUNT          763 a         1540 c         1448 g          682 t
ORIGIN      
        1 cccacctgtt cggaaaaggc gccggcgctg gtcatcacct gccatggccg gccggccggc
       61 ggccccttgg ccatgccttg ctgctgcatc cccatcggca tctcccggcg cgttgtgtgt
      121 tgggcgatta tcgccccatt gatcgtttat cgacaaatgt ccgacccgca ccctggtgtc
      181 aagccaaccg gcgatatgcg atacgggatt gtcaagcggg ccggactgga ataaatcgta
      241 cgtatgttac atattcgcac atattcactg gcgcgaacat gaaccgcggg cagaggaaaa
      301 aggatgagct acagcttcga gcagcacaag atcatcggcg cgatcgaggc cgatgacgac
      361 tattggcgcc accttgagga gggcgtgctg aaagtgccgc gctgcgccgg ctgccacacc
      421 tggacatggc cggcccattt ccgctgcggc acatgcggat cctggacgtt cgactgggtg
      481 gaggtggccg cccagggcct catcttcagc cacacccgca cccattacgc cttcgaccgc
      541 gtgctggagc gccgcgagga cgtgccctat gtgacgctgg tggtggaatt gccccatgcc
      601 ggcggcatcc gcctgatcgg cgtcctgcag ggcagcgacg agggccttgc catcggcgcg
      661 caagtgaccg gcagcatcga tccgcccagc gccaaaagca aacactaccc cgccctgcgc
      721 tggcaactgg cccgatgaac ggcccgcccg cccaggaagc ccctcttttc gggagatcac
      781 agtgagcagg atgcgtggcg caacggccat cgtcggtatc ggccaaaccc cttattacaa
      841 acgcggcacc tcgcccgagc cggagttgaa actggcgctg cgcgccatca cccaggccgc
      901 cgaggatgcc gggatcgatg tgcgcgacat cgatggcttc atttcctatg cctcggaacg
      961 cagcgatccg gccaagctga tgccggcgct gggcaccagg gcctgccgct ttgccgcgct
     1021 ggtgtggatc cacggcgggg cgctggccgg cacgctgaac atcgcggcag gggccatcct
     1081 gtcgggccag gcagaggtgg tggccatcta tcgctcgatg gcggaaggca acagccagcg
     1141 cctgcgcgtg gcggtggcgc aaaacgacac gtcgtcgcaa tatctcgtca atggcctcga
     1201 ttcgccggcg cagatcctgg ccctgcgcgc ccagcggctg atggaacatc atggcgtgcc
     1261 gcgttctgcg ctgtttgccg cggtgcaggc ctcctattac cacgcccggg caaaccccat
     1321 ggcctatggg cgcaacaccg agatgacccg cgagatctac gatgcagcgc ggccgatcgc
     1381 cgatccctat ggcctgttcg actgctcgcg cgagaatgac ggggcggcct gcgtgctggt
     1441 ggtttcggcc gacagggcca aggatctgcg tcagccgccg gcctatgtcc tgtcggcgcc
     1501 gatgggcacg atggaaggcg gcggcgcgct ggaggaaaac tggcgcccct attacgccac
     1561 ggccggccaa cgcgaactgg ccgaacggat gtggcaggaa gccggctatg gcccgggcga
     1621 tgtcgatgtc gcgcagatct acatgaacat gaatgccatg ggcatcggct cgatgatcga
     1681 ccatggcttc ttcagcttcg aggaagccgg cacgttctgc acctttgaga acctcatcgc
     1741 gccctcgggc cggttgccga tcaacacatc gggcggcgat ctggcggaag gcttcatcca
     1801 cggcatgggg ctgattccgg aggcggtgcg ccagatccgc ggcacttcca ccaaccaggt
     1861 gccgggcgcg aaactgtcgc tgttcaccgg cggcccgggc gatctcatga gcagcaccgc
     1921 ccttctgggc agcgccgaca ctctctgacc taacttgcag gtcttcgacc taacttgcgg
     1981 gaaagcacga aatggcattc acagtcagcc gcgcgctcaa atggtgggca tgggacaagc
     2041 ccgaccgcat cgcgctgaat tacgagggcg aggccctgac ctatgcgcag ctctacggct
     2101 gggcgctgcg cgtggccgac tggctgcgca cacagggcat ccagccgggc gagcgggtga
     2161 tgatggtggg cgagaatacg ctggactatg ccgtgctgct ggtgggcacg gtgctggccg
     2221 gcggcatctg cgcgccgatc accttccgct ccagcgagcg cgaattgcgc cgttcgctgg
     2281 aactgctctc cccccgcctc gtcgtggtcg acagggatcg cgaggagatc gccagcctgg
     2341 cgctgggcgg cgagggcaag ggccggctgg tgctgatgga cagcctgcgc gccctgcgca
     2401 gcgcgcccga accggcccct gcccacgagg cccggcccga ggatccgatg ttcatcatcg
     2461 gcaccagcgg ttccaccggc acccccaagg gcgtggtcta cacccagggt tccaccatgg
     2521 tctatgcggc ggaattcgcg atcatggagc cggccaccgg caatggcggc tcggtgcttt
     2581 cggccgggcc ctattcctcg gcctcgggca ccttgctgct gttccagttc ctgtcggtgg
     2641 gcgccacgat ctttgccgag aacaagttca cgcccgaacg cgcgctgcgc atgctgcgcg
     2701 acgagcgcat caccactttc ctcgcctcca ccatcttctt cgagcgcatt gccgcgctgc
     2761 ccgaatttcc cgccgccgat ctctcctcga tcaaattcgc ccagatcagc ggcgcccggg
     2821 tcagcaccgc gctgatcgcc cgcttccagg ccaagggcgt ggtgctgcgc caggcctatg
     2881 gctgcaccga ggccggcggc gcctgggccg cgcgcgatga aaccgcgctg gtggatccgg
     2941 aaaaatgcgg ccccggcgcc ctgttcaccg aatatgccat cgcccggccc gatggcaccc
     3001 gcgccgcgcc cggcgaggcg ggggaaatcc tcatccgcag cccggcgctg gccgccggct
     3061 actggcacga tgcagagcag accgcccgca ccttccgcgc aggctggctg cacaccggcg
     3121 accgcggcgt gcaggatgaa cgcggcaacc tcaccttcat cgaccgcatc aaggacatca
     3181 tcatctccgg cggcctcaat atctcggcca tggaagtgga aagcgccatc gccgagatcg
     3241 acggcgtggt ggaagtggtg gtgctggccg ccagagacgc cgaattcggc gaaacgccgc
     3301 tggctgtcgt gcatggcgat ctgggccgga tcgatgcccg ccagatcctg cagcattgcc
     3361 gccaggccct cgccacctac aaggtgcccc gctatatcgc gctggaagaa cggccgctgc
     3421 cgcgcctgcc ctcgggcaag atctccaagg tggaactgcg cgagaaatac aaggatgggc
     3481 cggcgtttct cgaaagggtg cgctaagagg cggcagcggg atgcaaggag aagcccttgc
     3541 atcccgttgc tcaatcgagt tcgataatcc gcgcggcagg gccggccaga tccaccagcg
     3601 cgcagctgcg ccgaaagccc tgcggcgtgg ggcgcccctc gcccaccgag cccgggttga
     3661 ccaccagcac cgggcccagc cagcgcacca gcggctgatg cgtgtggcca tacagaacga
     3721 catccgcccc ggccacggcg aagcggtgaa aatcgcggtg atggccgggc acataggcat
     3781 ggccggaagg ccagggcgtg gaatgaacga ccgtgagccg ccgcccgccc aaggcgatat
     3841 ccagccgctg cgggcaagcg gccagccagc cggccagccc gggatcgaca tccgcgcctg
     3901 tgggcccgcc ggcaaagaac aggtcctcgt ggttgccgcg gatcgtcagc gcggcctgcg
     3961 cccgcagcat ggccaccgtc tcgttgcaga agcgggtctg gttgatggca tcgcccaggc
     4021 acaccagccg gtcgatcggg cccatcagct ccagcgcgcg ggccagcgcc tgcggctggc
     4081 catgaatgtc ggagacgacc cccagccgca tgccggctca ggcatccacg ccggtggctg
     4141 gcgccgcatc cgtggcgcgc catggcgcca gcgccgcgcg cccgatctcg gccgccatat
     4201 aggccggccc gcctttccac gccggccaca ggccagcgcg caccatggca aaatccacat
     4261 ccgcagcgcg cagcgccacc ccttcgtcaa tcagcgccca tgcctcggcc agcaccggcc
     4321 agaccagcgc ctgctcgatc tgcgcgctgg ccgtgccggc cccctcggcc gccggcagca
     4381 aggcggcggc aaagccaaag gcccggcccg aggcggcgat atcggccggg cgg
//
LOCUS       DASRYE010000146         8362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_8264,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000146 DASRYE010000000
VERSION     DASRYE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8264"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFF98_07835"
     CDS             <1..693
                     /locus_tag="VFF98_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63578.1"
                     /translation="RAVALRLAADGARVAVNYRRDTAAAQAVVDEIRAAGGSAFALQA
                     DVGDLAAIAALFAGFDAAEPDGLDILVNNAGIGSMATLAETEEEAYDRQMQVNAKGPF
                     FVTRAALPRLRTGGRIVNVSSMVAQVAYPSCIAYAMSKAAINHFTRSLAAELGPRGIT
                     VNAVTPGATITDFAPELFNDPATVEYLAGMAALKRVGQPEDVARVIAMLAGPDAAWVT
                     GQVIEASGGMHL"
     gene            complement(720..974)
                     /locus_tag="VFF98_07840"
     CDS             complement(720..974)
                     /locus_tag="VFF98_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZU63579.1"
                     /translation="MTHTAHDLQAEFPADAALLHELKLSDAHYQKLAGRHHDINREIH
                     RIESESEAASDTRLEELKKARLSLLDEVAALLVRVRAATA"
     gene            complement(1044..1982)
                     /locus_tag="VFF98_07845"
     CDS             complement(1044..1982)
                     /locus_tag="VFF98_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63580.1"
                     /translation="MDLRQLRYFVMVANERNFTRAAERLHMAQPPLSRQIHQLEEELG
                     QPLFDRAARPLRLTAMGHLFYEQACQVLDRVEQMRGMMTRAAGLEKRRFVIGFVASTI
                     YARLPVLIRRFRALAPHVELVLVDLGSLEQIGALKEGRIDVGFGRIRFEDPAVRRAIL
                     REERLVAAVPATHPLAEATAPVPLARIAAEPQILYPNAHRPSYADQVLSLFHDSGLQP
                     RVAHEVRELQIAVGMVAAEEGVCLVPESVQQARSEDVRYIEVAEPLTSPIIMSYRQGD
                     TSRELAIMAAVVAKSYAEWGHKVAAAVQELAAGAAA"
     gene            2187..3083
                     /locus_tag="VFF98_07850"
     CDS             2187..3083
                     /locus_tag="VFF98_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZU63581.1"
                     /translation="MTEDFVTTPAIQALLDKACGIGAAGGQPRLKAILRDLLEATMRI
                     IVRHDISENEFWAAARYLAEGAGEIGLIMPGTGLEHFLDLYNDAKDAEAGLSGGTPRT
                     IEGPLYVAGAPLVAGDGEVDLTSDPDDTITLHMTGRVLGPDGEPVAGAILHVWHANSK
                     GWYSHFDPTGEQTPFNNRRRIQLGADGRYAFRSKMPNGYSVPPGGATDVLMQALGRHG
                     NRPAHVHFFVEAPGYRQLTTQINFGDDPFAADDFAFGTREGLLPVPSRQGDAAFIGFD
                     FHLQRAAQPADEHFSTRQRALA"
     gene            complement(3183..5576)
                     /locus_tag="VFF98_07855"
     CDS             complement(3183..5576)
                     /locus_tag="VFF98_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63582.1"
                     /translation="MWRCLLASGVVLPAMLVAGGARAEEAAANSAAAAEKAPADGQIG
                     DLVVYARKRPENAQSVPIPITTLGGVELSTQNLANFTDFQAKFPAFSVYLTNPKQLNL
                     GIRGIGNNGFNTDGIDGSVGIFVDGVYTGRQGMVSTDFNDIADVELLRGPQGTLFGKN
                     TTAGAVIINTAKPSFTFGAKGEVNIGNQGLRESKGSVTGTLIPDLLAFRLSGYYSETN
                     GNYKNLYNGQYQNARQGEGLRGQLLFTPASNLTVRLIGTWNHQGFNTMSPVITSIYNS
                     AALQTRMANAGYTLNTGSAANPVINLNSPLNARTDTYAASGTIDYDLGKGGTITAISA
                     WEHWKCYTNNDNDYTQLNAIPDYGSCNNEQQFSQELRWSTPKGGAIEATVGGFLSRQQ
                     LAVDSRIQFGNQYYIWAANPSTTDFPNVSGKTWAQGAYANALAGLGFASHGVFHTNTE
                     ALFGNVSWHPDKARRLSLDGGLRYTWEHRSQFYAGWVTQNTGNLTTAQLSDMSSAAAN
                     AALGSVNQALSDNALSGEGSISYKVLPDAMVYVKYARGYKSAGFNLLPVTDSRIIALG
                     GSQTVKGETADNFEAGIKSEWFHHRLLVNLTAFHTVVQNYQANESQGVGNTAIKFLAN
                     VGSLTSNGVELEAEAQLAKGLHAKGFIGFDHAYYSSFHNSVCPGEVASTVLTCDLTGR
                     QVAWTPKWTGDVSLDYTHPVARGADGYGLIDVNWRSAQNTTVTLDPLAWIAPYALVNA
                     RVGVKLAEGRFDLQLWSDNLLNKGYYINMLGLTKSTGIVQGYPGNPRSFGLRVRVSY"
     gene            complement(5728..6513)
                     /locus_tag="VFF98_07860"
     CDS             complement(5728..6513)
                     /locus_tag="VFF98_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU63583.1"
                     /translation="MPEPGAGAARGTIVLIHGAWQGSWAFAAWQPLLAAAGWHSVAVD
                     LPGNGINPEPLAADGLAAYTDHVVGVLRAQGRPCVVLGHSGGGQVAAQVAEAAPECVS
                     ALVFLVGMMLPSGMRFVDLLAHCRRLYPEADFAGITPFLKASADGRFTQVPPAAAREI
                     FLHDCPPEAAAAAALRLTPQPEWGRAVVPALTPQRYGSVPRIYVEALADRSIGIALQR
                     EMQALSPGARRITLPCGHVPQLACPGPLTDALCAVLEEIQADV"
     gene            complement(6506..7450)
                     /locus_tag="VFF98_07865"
     CDS             complement(6506..7450)
                     /locus_tag="VFF98_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZU63584.1"
                     /translation="MAEALTIPRIDGAALFAGDEATVAALRAAGQRHGFFYLVNHGID
                     PALIAAVFAEAQGFFALPMAEKAALDMAGSPCNRGYEPLRGQRLEAGSPPDLKEGFYI
                     GEDLAADDPRVLAGRFNMGANQWPAGRPAFRAAMSAYFAAASALAGQVMGGLARALGL
                     PADYFAAFATDPAATLRLLHYPPQPANPLPGEKGCGAHTDFGAITLLLQDASGGLQVQ
                     DVASGAWIDAPPVPGAFVVNLGDLIARWTNHHFHSNPHRVINLSGQERYSVPFFFTGN
                     PDYQVSCLPTCLAEGQAPLYPPITTEQHLADCYRRTYA"
     gene            complement(7551..>8362)
                     /locus_tag="VFF98_07870"
     CDS             complement(7551..>8362)
                     /locus_tag="VFF98_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63585.1"
                     /translation="LQLEEQLGVPLFDRLPAGLRLTPAGEIFADHVTKVLQDERRMQG
                     ALEELQGLQRGEIAIAAVEGVNGDLLPHVLEALMRRYPLLQIRQASGGSAVAAQAVVE
                     GDADIAICYSLGRNPALRQHALGRFALGAIMPPDHPLAGRATLRFAECAAYPLILARA
                     PLSIHDQMRQLVEGHKGPLQVKLETASVDLARQMCRRGIALAFQTRLGIEADLASGAL
                     RHVPLRDGAGRPILSELGIYASAHRSLPAALALAIEWLAAEIARREGEEAG"
BASE COUNT         1380 a         2828 c         2791 g         1363 t
ORIGIN      
        1 cgggccgtgg ccctgcggct ggcggccgat ggcgcgcgcg tggcggtgaa ctatcgccgc
       61 gacaccgccg cggcgcaagc cgtggtggac gagatccgcg ccgcaggcgg cagcgccttt
      121 gcgcttcagg ccgatgttgg cgatctggcg gcgatcgccg cgctgttcgc cggtttcgac
      181 gcggccgagc ctgacgggct cgacattctc gtcaacaatg ccggcatcgg ctcgatggcc
      241 accctcgccg aaaccgagga ggaggcctat gaccggcaga tgcaggtgaa cgccaagggc
      301 cccttcttcg tgacgcgggc ggcgctgccc cggctgcgta cgggcgggcg catcgtcaat
      361 gtgtcgtcaa tggtggcgca ggtcgcctat ccctcctgca ttgcctatgc gatgagcaag
      421 gcggcgatca accatttcac ccgctcgctg gcggcagagc tggggccgcg cggcatcacc
      481 gtcaatgcgg tcacgcccgg cgccaccatc accgatttcg cgccggaact gttcaacgat
      541 ccggccacgg tggaatatct ggcgggcatg gcagcgctga agcgcgtggg ccagccagag
      601 gatgtggcgc gggtgatcgc catgctggcc ggcccggatg ccgcctgggt aaccggccag
      661 gtgatcgagg catcgggcgg catgcacctc taaaggcgcg ccgcccgggg ccccatcggt
      721 caggccgtgg cggcgcgcac ccgcaccagc agcgcggcca cttcatccag cagcgagagg
      781 cgcgccttct tcagctcttc cagcctggtg tcggaagcgg cttccgattc gctttcgatg
      841 cggtggatct cgcggttgat gtcgtggtgg cgcccggcca gcttctggta atgcgcgtcc
      901 gacagtttca gctcgtgcag cagggcggca tcagcgggaa attcggcctg caggtcgtgg
      961 gcggtgtggg tcatactgtc tccctttcgg tgtctttcac gcacctctct cgcgccgatc
     1021 ggcgctggcc gcattgcgcc cgatcaagcg gccgcgccgg ccgccagttc ctgcacggcg
     1081 gccgccacct tgtgccccca ttccgcatag cttttggcca ccaccgcggc catgatcgcc
     1141 agctcgcgcg aggtatcgcc ctggcggtag ctcatgatga tcggcgaggt cagcggctct
     1201 gccacctcga tatagcggac atcctcgctg cgcgcctgct gcactgattc gggcaccagg
     1261 cacacgcctt cctcggccgc caccatgccc accgcgatct gcagctcgcg cacttcatgg
     1321 gccacgcgcg gctgcagccc gctgtcgtgg aagagcgaga gcacctgatc ggcatagctg
     1381 ggccgatggg cgttgggata gaggatctgc ggctcggcgg cgatgcgggc cagcggcacg
     1441 ggcgcagtag cctcggccaa cggatgggtg gccggcaccg cggccaccag ccgttcctcg
     1501 cgcaggatgg cgcggcgcac tgccggatct tcaaagcgga tgcgcccgaa gccgacatcg
     1561 atgcgcccct ccttcagggc gccgatctgc tccaggctgc ccagatccac cagcaccagc
     1621 tccacatgcg gcgccagcgc gcggaagcgg cggatcagca ccggcagccg cgcatagatg
     1681 gtggaggcga caaagccgat cacgaagcgc cgcttttcaa ggccggccgc gcgcgtcatc
     1741 atgccgcgca tctgctccac ccggtccagc acctggcagg cctgctcgta gaacagatgg
     1801 cccatggcag tgaggcgcaa ggggcgggcg gcgcggtcga acaggggctg gcccagttcc
     1861 tcctccagct ggtggatctg ccggctgagc ggcggctggg ccatgtgcag ccgctctgcc
     1921 gcgcgggtga aattgcgctc gttggccacc atgacgaaat agcgcagctg cctcagatcc
     1981 atcaccgata cccttcaggt attgcgcgca gaccgcattg atctttgcca cgcccgccga
     2041 tcctgcataa atccagggca gaaaagccgc aacggccaag aggatggcaa aatggcaagg
     2101 ccgcgccccg gcacccccgg cgccagacga tacctttgcc atcccctgcg ccccttgcga
     2161 caacaccccc gaacgggaga gaaggcatga ccgaggattt cgtcaccacg ccggccatcc
     2221 aggccctgct cgacaaggcc tgcggcatcg gcgctgccgg cggccagccg cgcctgaagg
     2281 cgatcctgcg cgatctgctg gaagcaacga tgcgcatcat cgtgcgccac gacatttccg
     2341 agaacgaatt ctgggcggcc gccagatatc tcgccgaagg cgccggcgag atcggcctga
     2401 tcatgccggg cacggggctg gaacatttcc tcgacctcta caacgatgcc aaggatgccg
     2461 aggccggcct cagcggcggc acgccgcgca ccatcgaggg gccgctctat gtggccggcg
     2521 ctccgctggt ggcaggcgat ggcgaggtgg acctcacctc cgaccccgac gacaccatta
     2581 ccctgcacat gaccggccgc gtgctgggcc ccgatggcga accggtggcc ggcgccatcc
     2641 tccatgtgtg gcacgccaat tccaagggct ggtattccca cttcgacccc accggcgagc
     2701 agaccccgtt caacaaccgc cgccgcattc agctgggcgc cgatggccgc tatgccttcc
     2761 gctcgaaaat gcccaacggc tatagcgtgc cgccgggcgg ggccaccgat gtgctgatgc
     2821 aggcgctggg ccgccatggc aaccggccgg cccatgtgca tttcttcgtg gaagcgcccg
     2881 gctatcgcca gctgaccacg cagatcaact ttggcgatga tccctttgcc gccgatgatt
     2941 tcgcctttgg cacccgcgag ggcctgctgc cggtgcccag ccgccagggc gatgccgcct
     3001 tcatcggctt cgacttccac ctccagcgcg cggcccagcc ggccgacgag catttctcca
     3061 cccgccagcg cgccctggcc tgaccggcat tgccctaaca cagggcctca atacgcccaa
     3121 acagaagggg cgggcgaagg gatcccctcg cccgcccctt ttttttcaga taggccgccc
     3181 gatcagtagc tcacccgcac ccgcaggccg aagctgcgcg gattgcccgg atagccctgc
     3241 acgatgccag tgctcttcgt cagccccagc atgttgatgt agtagccctt gttgagcagg
     3301 ttgtcggacc acagctggag gtcgaagcgg ccctcggcca gcttcacgcc cacccgcgca
     3361 ttcaccagcg cataaggcgc gatccacgcc agcggatcga gcgtgacggt ggtgttctgg
     3421 gccgagcgcc agttcacgtc gatcagccca tagccatcgg cgccccgcgc caccgggtgg
     3481 gtgtagtcca ggctcacgtc gccggtccac ttcggcgtcc aggccacctg ccggccggtc
     3541 aggtcgcagg tcagcacggt ggaggccact tcgcccgggc acaccgaatt gtggaacgag
     3601 ctgtaatagg catgatcgaa gccgatgaag cccttggcat gcaggccctt ggccagctgc
     3661 gcctcggctt ccagctccac accgttcgag gtgagcgagc cgacattggc gaggaacttg
     3721 atcgccgtgt tgcccacgcc ttgcgattca ttggcctgat agttctgcac caccgtgtgg
     3781 aaggcggtga ggttgaccag caggcggtgg tggaaccatt cgctcttgat gccggcctcg
     3841 aaattgtcgg cggtctcgcc cttcacggtc tggctgccgc ccagcgcgat gatgcgcgaa
     3901 tcggtcaccg gcagcaggtt gaaaccggcc gacttgtagc cgcgcgcata tttcacatag
     3961 accatcgcat ccggcagcac cttgtagctg atggagcctt cgcccgagag cgcattatcc
     4021 gacagcgcct ggttgaccga gcccagcgcc gcattggccg cggccgagga catgtcgctc
     4081 agctgggcgg tggtgaggtt gccggtgttc tgcgtcaccc agccggcata gaactggctg
     4141 cggtgttccc aggtgtagcg caggccgcca tccagcgaga ggcggcgggc cttgtccggg
     4201 tgccagctga cattgccgaa cagcgcctcg gtgttggtgt ggaacacgcc gtggctggca
     4261 aagccaaggc cggcaagggc gttggcatag gcgccctggg cccaggtctt gccgctcaca
     4321 ttgggaaagt cggtcgtcga ggggttggcc gcccagatgt aatactggtt gccgaactgg
     4381 atgcggctgt ccaccgccag ctgctggcgc gacaggaagc cgcccaccgt cgcctcgatc
     4441 gccccgccct tcggcgtgga ccagcgcagc tcctggctga actgctgctc gttgttgcac
     4501 gagccgtaat cggggatggc gttgagctgg gtgtagtcgt tgtcgttatt ggtgtagcac
     4561 ttccagtgct cccaggcgga gatcgcggtg atcgtgccgc ccttgcccag atcatagtcg
     4621 atcgtgccgc tggcggcata ggtgtcggtg cgcgcattca gcggcgagtt gaggttgatc
     4681 accggattgg cggcgctgcc ggtgttcagc gtgtagccgg cattggccat gcgcgtctgc
     4741 agggcggcgc tgttgtagat ggaggtgatc accggcgaca tggtgttgaa gccctggtgg
     4801 ttccaggtgc cgatcagccg cacggtgagg ttgctggccg gcgtgaacag caactggccg
     4861 cgcaggccct cgccctggcg ggcgttctga tactggccgt tgtagaggtt cttgtagttg
     4921 ccgttcgtct cgctgtaata gcccgacagg cggaaggcca gcaggtcggg gatcagcgtg
     4981 ccggtcacgc tgcccttgct ttcgcgcagg ccctggttgc cgatgttcac ctcgcccttg
     5041 gcgccgaagg tgaagctggg cttggccgtg ttgatgatga cagcgcccgc cgtggtgttc
     5101 ttgccaaaca gcgtgccctg cggcccgcgc agcagttcca catcggcgat atcgttgaaa
     5161 tcggtgctca ccatgccctg ccggccggtg tagacgccat cgacgaagat gcccaccgag
     5221 ccatcgatgc catcggtgtt gaaaccgttg ttgccgatgc cgcgaatgcc caggttcagc
     5281 tgcttggggt tggtgagata gaccgagaag gccgggaact tggcctggaa atcagtgaaa
     5341 ttggccaggt tctgggtaga cagctccacc ccgcccaggg tggtgatcgg aatcggcacc
     5401 gattgcgcgt tttccggccg cttgcgggca taaaccacca gatcgccgat ctggccatca
     5461 gccggggcct tttcggccgc agcggcgcta ttggcggcgg cctcttcggc ccgcgcaccg
     5521 ccggccacca gcatggcagg cagaacaaca ccagacgcca gaagacagcg ccacatatgc
     5581 gacgttttca tgttcttcac cccgtttttt caagacgcat gggagggcgc ccgccagcat
     5641 ggccggcaga caacatgcgt tttttcaaat tgttgcgggg ttcggtattg cggttacatt
     5701 ccgtatttat gacagggggg aaattctcta cacatctgct tggatttcct ccagcacagc
     5761 gcacagcgca tctgtcagcg gcccggggca ggccagttgc ggcacatggc cacagggcag
     5821 ggtgatccgc cgcgcgccgg ggctgagcgc ctgcatctcg cgctggaggg caatgccgat
     5881 cgaacgatcc gccagcgcct ccacatagat gcgcggcacg ctgccatagc gctgcggcgt
     5941 cagcgcgggc accaccgcgc ggccccattc gggctggggg gtgaggcgca gggcagcggc
     6001 cgccgcagcc tcgggcgggc aatcatgcag gaagatctcg cgcgcggcgg cgggcggcac
     6061 ctgcgtgaag cggccatcgg cgctggcctt gaggaagggg gtgatgccgg caaaatccgc
     6121 ttccggataa aggcggcggc aatgggccag caaatcgaca aagcgcatgc ccgagggcag
     6181 catcatcccc accagaaaca ccagcgcgga aacgcattcg ggcgccgctt ctgccacttg
     6241 cgctgccacc tgcccgccgc cgctgtggcc cagcaccacg caagggcgcc cctgcgcgcg
     6301 cagcacgccc accacatgat cggtgtaggc cgccagcccg tctgccgcca gcggttcagg
     6361 gttgatgcca ttgcccggca gatccaccgc cacgctgtgc cagccggccg cggccagcag
     6421 cggctgccag gcggcaaagg cccagctgcc ctgccaggcg ccatggatca gcacgatggt
     6481 gccccgggca gcgcccgcgc ccggctcagg cataggtgcg ccgatagcag tcggccagat
     6541 gctgctcggt ggtaatcggc ggatagagcg gggcctgccc ttcagcaagg caggtgggga
     6601 ggcacgacac ctgataatcg gggttgccgg tgaaaaagaa gggcaccgaa tagcgctcct
     6661 gccccgacag gttgatcacc cggtgggggt tggaatggaa atggtggttc gtccagcggg
     6721 caatcagatc gcccaggttg acgacgaaag cacccggcac cggcggcgcg tcgatccacg
     6781 cgccgctggc cacatcctgc acctgcaggc cgccgctggc atcctgcagc agcagggtaa
     6841 tcgcgccaaa atccgtatgc gcgccgcagc ccttctcgcc gggcaggggg ttggcgggct
     6901 ggggcggata atgcagcagg cgcaaggtgg cggccggatc ggtggcgaaa gcggcgaaat
     6961 aatcggccgg caggcccagc gcccgggcca gcccgcccat cacctgcccg gccagcgcgc
     7021 tggcggcagc gaaataggcg ctcatcgccg cgcggaaggc ggggcggccg gcgggccact
     7081 ggttggcgcc catgttgaag cggccggcca gcacgcgcgg atcatcggca gccagatcct
     7141 cgccgatgta gaagccttcc ttcaggtcgg gcgggctgcc cgcctccagc cgctgcccgc
     7201 gcaagggctc atagccgcgg ttgcagggcg agccggccat gtcgagcgcc gccttctcgg
     7261 ccatgggcag ggcgaagaag ccctgcgcct cggcaaagac ggcggcgatc agcgccggat
     7321 cgatgccatg gttaacgaga tagaagaagc cgtggcgctg gcccgcggcg cgcagggcgg
     7381 ccacggtggc ctcatcgccg gcaaacagcg cggccccgtc gatccgggga atggtaagag
     7441 cttcggccat atgcgtttcc tgccctgaag tggctgtgga acgcaggata ggcatggccc
     7501 ggccgctgcc gccagaacca aattccgcca tggccgatgc cgtttcggca tcacccggcc
     7561 tcttcgccct cgcgccgggc gatctcggcc gccagccatt cgatggccag cgccagggcc
     7621 gccggcaggc tgcgatgggc gctggcatag atgcccagtt cggaaaggat cggccggccg
     7681 gccccgtcgc gcagcggcac atggcgcagg gcgccgctgg ccagatccgc ctctatgccc
     7741 agccgcgtct ggaaggcgag ggcaatgccg cgccggcaca tctgccgcgc cagatcgaca
     7801 gaggcggtct ccagcttcac ttgcagcggg cccttgtgcc cctccaccag ctggcgcatc
     7861 tgatcgtgga tggacagcgg cgcgcgcgcc agaatcagcg gataggccgc gcattcggca
     7921 aagcgcaggg tggcgcgccc ggccagcgga tgatcgggcg gcatgatcgc ccccagcgcg
     7981 aaacggccca gcgcatgttg ccgcaacgcc ggattgcgcc ccaggctgta gcagatggcg
     8041 atatcggcat cgccttccac caccgcctgc gccgccaccg ccgagccgcc cgaggcctgg
     8101 cggatctgca gcagcggata gcggcgcatc agcgcttcca gcacatgggg caaaaggtcg
     8161 ccgttcactc cttccaccgc ggcaatggcg atctcgccgc gctgcaggcc ctgcagctct
     8221 tccagcgcgc cctgcatgcg gcgctcgtcc tgcagcacct tggtcacgtg gtcggcgaag
     8281 atctcgcccg ccggggtcag ccgcaggccg gcgggcaggc gatcgaacag cggcacgccc
     8341 agctgctctt ccaactgcag ca
//
LOCUS       DASRYE010000147        14072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_131872,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000147 DASRYE010000000
VERSION     DASRYE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131872"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /gene="gorA"
                     /locus_tag="VFF98_07875"
     CDS             <1..1309
                     /gene="gorA"
                     /locus_tag="VFF98_07875"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HZU63586.1"
                     /translation="SGGVRASRIAASHGASVAVAEEYRIGGTCVIRGCVPKKLLVYAS
                     QFAEELHDTARLGWTIGEKRFDWAALRDFVASDVDRLERAYTNTLDSNKVAHFAERAV
                     VTGPNSLRLVPSGREITARYILVAVGAWPVMPDFPGNEHAITSNEVFHLPELPRRVVV
                     QGAGYIALEFAGIFNALGCAVAVVNRSDRILRGYEEALTERLLKITQERGIAYRLGDP
                     VAGIEKQADGSLAVALASGAVIAADCVLVATGRKPKTDGLGLESAGVALGGNGEVLVD
                     PYGETSCPSIYAVGDVTDRVQLTPVAIREGHAFADTVFGGKPRTTAYDHIPSAVFSQP
                     PLAGVGLTEAEARGQGYKVQIFTSDFRPMKNMFAARAERGFYKLVVEEATQRVLGVHM
                     IGPDAPEILQAVAIAVKAGLTKADFDATVALHPSMAEELVLMR"
     gene            complement(1317..1682)
                     /locus_tag="VFF98_07880"
     CDS             complement(1317..1682)
                     /locus_tag="VFF98_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63587.1"
                     /translation="MTPHSSPALAHAQGFALARAEAAIDLMLDILSDGCDHPELWADM
                     AELSFLYPGLRERIAGRYYAAAPGLCATRLLILHALCAAAEGDLAIARAEIEPLALCH
                     GQQALVQGARAWLHRHPGH"
     gene            1910..3436
                     /locus_tag="VFF98_07885"
     CDS             1910..3436
                     /locus_tag="VFF98_07885"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZU63588.1"
                     /translation="MSANIAEMEKRREAAKLGGGQRRIDAQHAKGKLTARERIEVLLD
                     EGSFEELDMYVEHNCVDFGMPDQVIPGDGVVTGSGTINGRLVFVFSQDFTVFGGSLSE
                     RHAQKICKVMDMAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVNASGVVPQL
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPDVVKTVTNEIVTQEELGGAITHT
                     TKTSVADLALENDIEALLAAREFFDFLPLSNREDAPARPNSDPWDRADGSLDTLIPAS
                     ATQPYDMHEVIRKTLDEGDFFEIQPGHAGNILIGFGRIEGRTVGVVANQPMVLAGCLD
                     INSSKKAARFVRFCDAFNIPIITFVDVPGFLPGTSQEHNGIIKHGAKLLFAYAEATVP
                     KITVITRKAYGGAYDVMASKHLRGDLNYAWPTAEIAVMGAKGAVEIIFRGLSGEEQAE
                     KTKEYEERFANPFVAASKGFIDEVIYPHSTRKRIALGLRKLRNKALENPWKKHDNIPL
                     "
     gene            3572..4759
                     /gene="bktB"
                     /locus_tag="VFF98_07890"
     CDS             3572..4759
                     /gene="bktB"
                     /locus_tag="VFF98_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HZU63589.1"
                     /translation="MSEAQDIFIVSGVRTAIGDFGGALKGFAASDLGARVVAEAVNRA
                     GLQPADIGHVVLGQVFITGAKDAYIARVAALNAGVPVETPALTVNRLCGSGVQAIISA
                     AQMMKLGEASITVAGGAESMSNVPYHDHGVRWGKKMGNNVLVDALTLGLSDPIGEYHM
                     GVTAENVAEKCGVSREAQDQLAVEGHVRAARAIAEGRFKEQILPIEVKTRKGVTVFDT
                     DEHPRADTTLEALAAMKAVFKKDGSVTAANASGINDGAAAVVLATGAEVEKRGLAPLA
                     RIVAWGHAGVEPHLMGLGPVKAVPVALARAGLTLADIDVIEANEAFAAQALAVARELG
                     FDSEKLNPNGSGVALGHPVGATGAILTVKAAYELKRTGGRYGLITMCIGGGQGIAMII
                     ERV"
     gene            4765..5217
                     /gene="mce"
                     /locus_tag="VFF98_07895"
     CDS             4765..5217
                     /gene="mce"
                     /locus_tag="VFF98_07895"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZU63590.1"
                     /translation="MKLGRLNHVGVAVPAMADAIAFYRDVLGAEVITEPFDLPEQGVK
                     VAFVNTPPQPGALGSIGGTQIELIEPLGESSPIAAFIAKNPAGGQHHLCYEVEDIAAA
                     RAWFEGAGKRILGPTRIGAHGTPIFFVHPKDMLGQLTEIMERPQGEHH"
     gene            5217..6008
                     /locus_tag="VFF98_07900"
     CDS             5217..6008
                     /locus_tag="VFF98_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU63591.1"
                     /translation="MAAWRFITAEAEGAVTTLSLNRPDKLNALTPAVFAELAQAIDAA
                     LAAGARALVLTGAGRFFCSGADIQPDGAGYEGLPEDLGALIEADYNPFMRKLASLPVP
                     LVTALNGPAAGAGVSLALSGDIVVAGEGAYLLLAFVNIGLVPDAGASWLLARGAGRAR
                     ALEMALLGEKLPAAEAKAAGLVSRVVAEGEVLAEAQKLAARLAAGPTKAIGLIRRQIA
                     FALDHGFDAALDEERDNQRIVGRSADFAEAIAAFGEKRKARFRGE"
     gene            6024..8162
                     /gene="scpA"
                     /locus_tag="VFF98_07905"
     CDS             6024..8162
                     /gene="scpA"
                     /locus_tag="VFF98_07905"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HZU63592.1"
                     /translation="MSDLDKWQALAAKEVKGKDLIWHTPEGIAVKPLYTADDVAGQEP
                     GLPGFAPFTRGVRASMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFDLA
                     THRGYDSDHPRVVGDVGKAGVAIDSVEDMKILFDGIPLGEMSVSMTMNGAVIPILAFF
                     IVAGEEQGVPTEKLDGTIQNDILKEFMVRNTYIYPPEPSMRIISDIFAFTSANMPKFN
                     SISISGYHMQEAGATQVQELAFTIADGMEYVKYGVASGLDIDKFAGRLSFFFAIGMNF
                     FMEVAKLRAARVLWHRVMTKLGAKDERSKMLRTHCQTSGVSLQEQDPYNNVIRTTIEA
                     MAAMLGGTQSLHTNALDEAIALPTDFSARIARNTQIVIQEETGMCNVVDPLGGSYYIE
                     SLTQSLVDAAWEIIERVDGEGGMAKAVAAGWPKAMIEEAAAGRQARVDKGDDVIVGVN
                     KYRLATEDRIETLEVDNHVVREAQIARINAVKAARDEAACQAALTAITEGAQSGGNLL
                     ALAVVAARARATLGEISAAMEAVFGRHGTQPSPVKGIYGAAYAGDSRYAQVVDGVGAV
                     SRRLGRAPRVLVAKMGQDGHDRGANVIASAFSDMGFEVTSGPLFQTPEEACQLALAKD
                     VDAVGASSLAAGHKTLIPELIGHLRAAGRADIKVVAGGVIPPQDYDFLREAGVQGIYG
                     PGSNVVECAADLLRLLGHNMPPLGEALAAE"
     gene            8202..9278
                     /gene="bioB"
                     /locus_tag="VFF98_07910"
     CDS             8202..9278
                     /gene="bioB"
                     /locus_tag="VFF98_07910"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZU63593.1"
                     /translation="MPGNSPPFGDDEVSAIRNDWTRDEIAALFDLPFTELVFRAAEVH
                     RANHPANEVQLSTLLSIKTGGCVEDCGYCSQSVKADSGVKPTKLMEVQAVLQAAARAK
                     DNGSSRFCMGAAWRNPKARDMPAIIDMVKGVRAMGLETCMTLGMLEPEQADMLAEAGL
                     DYYNHNIDTSPERYNEVITTRTMEDRLDTLGEVRRAGINVCSGGIVGMGETRADRVGF
                     VHTLATLPSHPQSVPINALVPVKGTVLGDMLADTPLAKIDDVEFVRTVAAARIAMPGS
                     MVRLSAGRESMSEMTQAMCFLAGANSIFTGDTLLTAPNAGDDKDAAMFARLGIKAMAV
                     TRTEAELAAERMPRGCAKLEQAAE"
     gene            9327..11363
                     /locus_tag="VFF98_07915"
     CDS             9327..11363
                     /locus_tag="VFF98_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HZU63594.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIATVAVYSDADARAPFVRM
                     ADEAVHIGPSPAAQSYLIADKIIAACKATGAEAVHPGYGFLSERTSFAEALAKENIAF
                     IGPPVGAIAAMGDKIESKKLAKSAGVNVVPGFVGEIRDTDHAVEISDEIGYPVMMKAS
                     AGGGGKGMRLAFSEKDVREGFESVKREGLNSFGDDRVFIEKFIQDPRHIEIQILGDQH
                     GNILYLNERECSIQRRHQKVVEEAPSPFVSAAMRKAMGEQCVALSRAVGYYSAGTVEL
                     IVSGADKTGESFYFLEMNTRLQVEHPVTEAITGIDLVEQMIRVAAGEKLAFTQDDIKI
                     DGWAIENRVYAEDPYRGFLPSTGRLVRYQPPVEGWSDDGAENANNLNGRRGIDGVRVD
                     DGVYEGGEVSMFYDPMIAKLITWGKTRDEAADKQIAALDAFEIEGLGHNIDFVSAIMQ
                     HPRFRSGELTTGFIAEEYPEGFHGAATAEELKRKLAAVAGFVATARADRARRIEGQLD
                     GLLPPPAGWSVQVAGQRFAVEVDEDAVSVDGAEVDLVMEYTPGDRLIAAEVDGEALTI
                     KVAATRTGLKFTTRGAIHVIDILPDHVAHLANHMIEKTPPDLSKFLICPMPGLLVALH
                     VAEGDKVEAGQPLAVVEAMKMENILRAEKSATVKKLNAKAGESLAVDAIILELE"
     gene            complement(11418..12524)
                     /gene="aroB"
                     /locus_tag="VFF98_07920"
     CDS             complement(11418..12524)
                     /gene="aroB"
                     /locus_tag="VFF98_07920"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZU63595.1"
                     /translation="MHTIPVDIAGRPYEVRVGRGLLADLAGQCGTRLRKAQVPIVTDE
                     RVHAHWGAVVEASLAAAGKQALWRILPAGEATKNWDNLIGTVEWLLAHEVERGDHILA
                     LGGGVIGDLTGFAASMLKRGCHFIQLPTTLLAQVDSSVGGKTAINAAAGKNLVGAFHQ
                     PALVLADLDALATLPSRELGAGYAEVVKYGLIDDAPFFAWCEENGAKVLAGDVAAQAH
                     AVAHSVAAKARIVAEDEFETTGRRALLNLGHTFGHALEAETGFSSRLLHGEGVALGMV
                     LAARFSARRGLIAAEVAERIAAHIAAVGLPADLRALGLNVSGADLAAHMLHDKKVDAG
                     RLAFLLLRGIGETFLTKDVTLAEVTAFLTDELAG"
     gene            complement(12554..13204)
                     /locus_tag="VFF98_07925"
     CDS             complement(12554..13204)
                     /locus_tag="VFF98_07925"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZU63596.1"
                     /translation="MDHEHPLPGPDEMSLIAQRITRPVVLVGMMGVGKSSLGRKLAAQ
                     LGAPFLDADEEIEKAAQMSIPEIFERHGEAFFRDGERRVIARLLGADVGASDHRLPLP
                     AHAPSKGVKVIATGGGAFCNTETRALILDKAIAVWLDADLDTLVERTARKDNRPLLRQ
                     GNPRDILGQLKDQRAPFYAEAPIRVMSGHAPHAKTLSRLFAAFDKWLPAQPATTGS"
     gene            13315..13521
                     /locus_tag="VFF98_07930"
     CDS             13315..13521
                     /locus_tag="VFF98_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63597.1"
                     /translation="MTIRIKRYPKSTWPTGFRQGAQRRPRLAAGLGVVLALAGSLMWM
                     AWRAGGPVARHEVVLALPAGEVGR"
     gene            13518..>14072
                     /locus_tag="VFF98_07935"
     CDS             13518..>14072
                     /locus_tag="VFF98_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63598.1"
                     /translation="MSGLRRLLLGAGLLAGLAGAALAQQGPESLLPPGFDRPARPSRS
                     PQPPAPARSAAPVAMAAPVIQPQTLFGGPPAPPPGALLGAVPLPAGVTSFNQLMALPQ
                     DQIDQLLGLTTKDDIPAGARHAMRQVGLMNQAEGGLPPFSLDHQDAGLVRAALAGNNG
                     HMVSRWGHILLRRALASRLDAPEGL"
BASE COUNT         2384 a         4380 c         5043 g         2265 t
ORIGIN      
        1 ctctggcggg gtgcgggcaa gccgcattgc cgccagccat ggcgccagcg tggccgtggc
       61 cgaggaatat cgcattggcg gcacctgtgt gatccgtggc tgcgtgccca agaagctgct
      121 ggtctatgcc agccagtttg ccgaggaact gcacgacacc gcccggctgg gctggacgat
      181 cggcgagaag cgcttcgact gggcggccct gcgcgatttc gtcgcctccg atgtggaccg
      241 gctggagcgc gcctatacca acacgctcga cagcaacaag gtggcgcatt tcgccgagcg
      301 cgcggtggtg accggcccca acagcctgcg cctggtcccc tcgggccgcg agatcaccgc
      361 gcgctacatt ctggtggcag tgggcgcctg gccggtgatg cccgattttc ccggcaacga
      421 acatgccatc accagcaacg aggtgttcca tttgcccgaa ctgccgcgcc gcgtggtggt
      481 gcagggggcg ggctatatcg cgctggaatt tgccgggatc ttcaatgcgc tgggctgcgc
      541 ggtagcggtc gtcaaccggt ccgacaggat cttgcgcggc tatgaggagg cgctgaccga
      601 gcgcctgctg aagatcacgc aggagcgcgg cattgcctat cgcctgggcg atccggtggc
      661 gggcatcgag aagcaggccg atggcagcct ggccgtggcg ctggccagcg gcgcggtgat
      721 cgcggccgat tgcgtgctgg tggccaccgg ccgcaagccc aagaccgatg ggctggggct
      781 ggaaagcgcg ggcgtggcgc tgggcggcaa tggcgaggtg ctggtcgatc cctatggcga
      841 gacgagctgc cccagcatct atgccgtggg cgatgtgacc gaccgcgtgc aactgacgcc
      901 ggtggccatc cgcgagggcc atgcctttgc cgacaccgtg ttcggcggca agccgcgcac
      961 caccgcctat gaccatatcc ccagcgcggt gttcagccag cccccgctgg ccggcgtcgg
     1021 cctgaccgag gccgaggccc gcggtcaggg ctacaaggtg cagatcttca ccagcgattt
     1081 ccgcccgatg aagaacatgt tcgccgcccg ggccgagcgc ggcttctaca agctggtggt
     1141 ggaagaggcc acccagcggg tgctgggcgt gcacatgatc ggcccggatg cgcccgagat
     1201 cctgcaggcg gtggcgatcg cggtgaaggc ggggctgacc aaggcggatt tcgatgccac
     1261 cgtggcgctg cacccttcca tggccgaaga gctggtgctg atgcgctgat cgggcgttaa
     1321 tggcccgggt ggcggtgcag ccaggcccgg gccccctgca ccagcgcctg ctggccatgg
     1381 cagagggcca gcggctcgat ttcggcgcgg gcgatcgcca ggtcgccctc tgccgccgcg
     1441 cacagcgcat gcaggatcag cagccttgtg gcgcaaaggc cgggcgctgc ggcataatag
     1501 cggccagcaa tcctttcgcg cagccccgga tagaggaagc tgagctcggc catgtcggcc
     1561 cacagctcgg gatggtcgca cccgtcggac aggatgtcga gcatgaggtc gatcgccgct
     1621 tcggcgcggg ccagcgcgaa tccctgcgca tgcgccaggg caggggagga atgcggtgtc
     1681 atcggcgcct cgctgtgtgg cgggaagggg tgcgggccag tctaggcaga cagggttagc
     1741 caagctttgg cgcgcgccta gggccgttta cggggccggc taatagcggg agcgccctat
     1801 tctttttaac cggccgctta aaaatcattg acgcgccccg ctggccgtgc cttaggcatt
     1861 gccaacaaac tggctttaag ggcctggctt aagagaggtc ggagcgcgca tgtctgcgaa
     1921 tatcgccgaa atggaaaaac gccgcgaagc agccaagctg gggggcggcc agcgccgcat
     1981 cgatgcccag catgccaagg gcaagctgac agcgcgcgag cgcatcgagg tgttgctgga
     2041 tgagggcagc ttcgaagagc tcgacatgta tgtggagcat aattgcgttg atttcggcat
     2101 gcccgatcag gtgatcccgg gcgatggcgt ggtgaccggt tcgggcacga tcaacggccg
     2161 cctcgtcttc gtcttctcgc aggatttcac cgtttttggc ggttcgctct cggaacgcca
     2221 cgcgcagaag atctgcaagg tgatggatat ggcgatgaag gtgggcgcgc ctgtcatcgg
     2281 cctcaacgat tcgggcggcg cgcgcatcca ggaaggcgtt gcctcgctgg gtggctatgc
     2341 cgaagtgttc cagcgcaatg tcaacgcctc gggcgtggtg ccgcagctca gcctcatcat
     2401 ggggccttgc gcgggcggcg ctgtgtatag cccggcgatg accgacttca tcttcatggt
     2461 gaaggacagc agctacatgt tcgttaccgg ccccgatgtg gtgaagacgg tgaccaacga
     2521 gatcgtgacc caggaagagc tgggcggggc gatcacccac accaccaaga cttcggtggc
     2581 ggatctggcg ctggagaacg atatcgaggc gctgctggca gcgcgcgaat tcttcgattt
     2641 cctgccgctg tcgaaccgcg aggatgcgcc cgcacgcccc aattcggacc cgtgggaccg
     2701 cgccgatggc agcctcgaca cgctgatccc ggcttctgcc acccagccct acgacatgca
     2761 tgaggtgatc cgcaaaaccc tcgatgaggg ggatttcttc gagatccagc cgggccatgc
     2821 gggcaatatc ctgatcggct ttggccgcat cgaggggcgc acggtgggcg tggtggccaa
     2881 ccagccgatg gtgctggccg gctgcctcga catcaattcc tcgaagaagg cagcgcgttt
     2941 cgtgcgcttc tgcgatgcct tcaacatccc gatcatcacc ttcgtcgatg tgcccggctt
     3001 cctgcccggc acctcgcagg agcataatgg catcatcaag catggcgcca agctgctgtt
     3061 cgcctatgcc gaggccaccg tgcccaagat caccgtgatc acccgcaagg cctatggcgg
     3121 cgcttacgac gtgatggcct ccaagcatct gcgcggcgac ctcaactatg cctggcccac
     3181 cgccgagatc gccgtgatgg gcgccaaggg cgcggtggag atcatattcc gcggcctttc
     3241 cggcgaggaa caggccgaga agacgaagga atatgaagag cgctttgcca accccttcgt
     3301 ggcggccagc aagggcttca tcgacgaggt gatctacccg cactccaccc gcaagcgcat
     3361 cgcccttggc ctgcgcaagc tgcggaacaa ggcgctggag aacccctgga aaaagcacga
     3421 caacatcccg ctgtaaacgc ggggaagcac gccggctggt ctttctcgca ggtgcagcga
     3481 gggcccgccg ggtgtttgca agggtgagga ccgggcgggc cgccggcaac aggcggccag
     3541 aggatgccgg caaggacaaa gggcaagaac aatgagtgaa gcgcaggaca tcttcatcgt
     3601 ttcgggcgtg cgcacggcca tcggcgattt cggcggcgcg ctgaagggct ttgccgccag
     3661 cgatctgggc gcgcgcgtgg tggccgaggc ggtgaaccgc gccgggctgc agccggcaga
     3721 cattggccat gtggtgctgg gccaggtgtt catcaccggc gccaaggatg cctatatcgc
     3781 ccgcgtggcg gcgctgaacg ccggcgttcc ggtggaaacc ccggcgctga cggtgaaccg
     3841 cctgtgtggt tctggcgtgc aggcgatcat ttcggcggcg cagatgatga agctgggcga
     3901 ggcctcgatc acggtggccg gcggcgccga gagcatgtcg aacgtgcctt atcatgacca
     3961 tggcgtgcgc tggggcaaga agatgggcaa caatgtgctc gtcgatgcgc tgacgctggg
     4021 cctgtcggat ccgatcggcg aatatcacat gggcgtgacg gccgagaacg tggccgagaa
     4081 gtgcggtgtg agccgcgagg cgcaggacca gttggccgtg gaaggccatg tccgcgctgc
     4141 ccgcgccatt gccgagggcc gcttcaagga gcagatcctg ccgatcgagg tgaagacccg
     4201 caagggtgtt accgtattcg acaccgacga gcacccgcgc gccgacacca cgctggaagc
     4261 gctggcggcg atgaaggcgg tgttcaagaa ggacggttcg gtgaccgcgg ccaatgccag
     4321 tggcatcaac gatggcgcgg cggccgtggt gctggccact ggcgccgagg tggagaagcg
     4381 cggtctggcg ccgctggcgc gcatcgtggc ctgggggcat gccggggtgg agccgcatct
     4441 gatgggcctg ggcccggtga aggcggtgcc ggtggcgctg gcgcgggccg ggctgacgct
     4501 ggccgacatc gatgtgatcg aggccaacga ggcctttgcc gcgcaggcgc tggccgtggc
     4561 ccgcgaactg ggcttcgaca gcgagaagct gaaccccaat ggcagcggcg tggcgctggg
     4621 ccatccggtg ggcgccacgg gcgccatcct caccgtgaag gccgcctacg agctgaagcg
     4681 caccggcggg cgctatggcc tcatcaccat gtgcattggt ggcgggcagg gcatcgccat
     4741 gatcatcgaa agggtctgat cgtcatgaag ctgggccgtt tgaaccatgt cggcgtggcg
     4801 gtgcctgcca tggccgatgc catcgccttc tatcgcgatg tgctgggcgc cgaggtgatc
     4861 accgagccgt tcgacctgcc cgagcagggg gtgaaggtgg cctttgtcaa cacgccgccg
     4921 cagccgggtg cgctgggcag cattggcggc acccagatcg agctgatcga gcctctgggc
     4981 gagagcagcc cgatcgccgc cttcattgcc aagaacccgg ccggcggcca gcatcacctg
     5041 tgctatgagg tggaagatat cgccgccgcg cgcgcctggt tcgagggcgc cggcaagcgc
     5101 attctgggcc ccacgcgcat cggcgcccat ggcacgccga tcttcttcgt ccaccccaag
     5161 gacatgctcg gccagctgac cgagatcatg gagcgaccgc aaggcgagca ccactgatgg
     5221 cggcctggcg cttcatcacc gccgaggcgg aaggggcggt gacgaccctc agcctcaacc
     5281 ggcccgacaa gctgaacgcg ctgacgccgg cggtgtttgc cgagctggcg caggcgatcg
     5341 atgcggcgct ggctgccggc gcgcgcgcgc tggtgttgac aggggccggg cggttcttct
     5401 gctcgggcgc cgatatccag cccgatggcg ccggctatga aggcctgccg gaggatctgg
     5461 gggcgctgat cgaggcggat tacaacccct tcatgcgcaa gcttgccagc ctgccggtgc
     5521 cgctggtgac ggcgctgaac gggccggcgg cgggcgcggg ggtgagcctg gcgctttccg
     5581 gcgatatcgt cgtggcgggc gagggggcct atctgctgct ggctttcgtc aacatcggcc
     5641 tggtgcccga tgccggggcc agctggctgc tggcgcgcgg cgccggccgg gcccgcgcgc
     5701 tggaaatggc gttgctgggc gagaagctgc cggcggcaga agcgaaggcg gccggccttg
     5761 ttagccgcgt ggtggcagag ggcgaggtgc tggccgaggc gcagaagctg gccgcccggc
     5821 ttgccgcggg gccaaccaag gcgatcggcc tgatccgccg tcagatcgcc ttcgcgctcg
     5881 accatggctt tgatgccgcg ctcgacgagg agcgcgacaa tcagcgtatt gtgggccgca
     5941 gcgccgattt tgccgaggcg atcgctgcct ttggcgagaa gcgcaaggcc cggttccggg
     6001 gtgaatgagg aggtgtcgcg ggtatgagcg atctcgacaa gtggcaggcg ctggccgcca
     6061 aggaagtgaa gggcaaggac ctgatctggc acacgccgga agggattgcc gtcaagccac
     6121 tctacacggc cgatgacgtg gccggccaag agccgggcct gccgggcttt gcccccttca
     6181 cgcgcggcgt gcgggccagc atgtatgccg gccgcccctg gacgatccgc cagtatgcgg
     6241 gcttttccac cgccgaggaa tcgaacgcct tctatcgccg caatctggcg gcggggcaaa
     6301 agggcctgtc ggtggccttc gatctggcca cgcaccgcgg ctatgacagc gaccacccgc
     6361 gcgtggtggg cgatgtgggc aaggccggcg ttgccatcga cagcgtcgag gacatgaaga
     6421 tcctgtttga cggcattccg ctgggcgaga tgtcggtgtc gatgacgatg aacggcgcgg
     6481 tgatcccgat cctcgccttc ttcatcgtgg ccggcgagga acagggcgtt cccaccgaaa
     6541 agctggacgg aaccattcag aacgacattc tgaaggagtt catggtccgc aacacctata
     6601 tctatccgcc cgaaccctcg atgcggatca tctcggacat tttcgctttc acatcggcga
     6661 acatgcccaa gttcaattcc atttccatct ccggctatca catgcaggaa gccggtgcca
     6721 cgcaggtgca ggaactggcc ttcaccatcg ccgatggcat ggaatatgtg aaatatggcg
     6781 tggccagcgg gctggatatc gacaagttcg ccggccggct gagcttcttc ttcgccatcg
     6841 gcatgaactt cttcatggaa gtggccaagc tgcgggccgc gcgcgtgctg tggcaccggg
     6901 tgatgacgaa gctgggcgcg aaggacgagc gcagcaagat gctgcgcacc cactgccaga
     6961 ccagcggcgt gagcttgcag gagcaggacc cctacaacaa cgtgatccgc acgacgatcg
     7021 aggcgatggc cgcgatgctg ggcggcaccc agagcctgca caccaacgcg ctggacgagg
     7081 cgattgcgct gcccaccgat ttttcggcgc gcattgcccg caacacgcag atcgtcatcc
     7141 aggaagagac cggcatgtgc aatgtcgtcg atccgctggg cggctcgtac tacattgaaa
     7201 gcctgacgca atcgctggtc gatgcggcct gggagatcat cgagcgggtg gatggcgagg
     7261 gcggcatggc caaggccgtg gccgccggtt ggcccaaggc gatgatcgag gaagcggccg
     7321 ctggccggca ggcgcgggtg gacaagggcg atgatgtcat cgtcggcgtc aacaaatatc
     7381 gcctggcgac ggaagaccgg atcgagacgc tggaagtgga caatcatgtt gtccgcgagg
     7441 cgcagatcgc tcgcatcaac gcggtgaagg ccgcgcgcga cgaggcggcc tgccaggcgg
     7501 cgctgacggc gatcacggag ggcgcgcaga gcggcggcaa cctgctggcg ctggccgtgg
     7561 tggccgcgcg ggcgcgggcc acgctgggcg agatttccgc ggcgatggaa gccgtgttcg
     7621 gccgccatgg cacccagccc agcccggtga agggcatcta tggcgccgcc tatgccggtg
     7681 acagccgtta tgcgcaggtg gtcgacgggg tgggcgcggt gtcgcgccgg ctgggccggg
     7741 cgccgcgcgt gctggtcgcc aagatgggcc aggacgggca tgaccgcggc gccaatgtca
     7801 tcgcctcggc cttctccgac atgggcttcg aggtgaccag cggcccgctg ttccagacgc
     7861 ccgaggaagc ctgccagctg gccctggcca aggacgtgga cgcggtgggc gcctccagcc
     7921 ttgccgccgg ccacaagacg ctgatccccg agctgatcgg ccatctgcgc gcggccggcc
     7981 gggccgacat caaggtggtg gccggcggcg tgatcccccc gcaggattac gacttcctgc
     8041 gcgaggcggg cgtgcagggc atctatgggc cgggcagcaa tgtggtggaa tgcgccgccg
     8101 acctgctgcg cctgctgggc cacaacatgc ctccgctggg cgaggcactg gcggcggaat
     8161 agggcaggcc gggggttttg cctggcgcgg cgaaatcgcc tatgccgggc aattcccccc
     8221 ctttcggaga cgatgaagtg agcgcaatcc gcaacgactg gacccgcgac gagatcgccg
     8281 ccctgttcga cctgccgttt accgagctgg tgttccgcgc ggccgaagtg caccgggcca
     8341 accatccggc caatgaggtg cagctgtcca cgctgctgtc gatcaagacg ggcggctgcg
     8401 tcgaggattg tggctattgc tcgcaatcgg tgaaggccga cagcggggtg aagccgacca
     8461 agctgatgga agtgcaggcg gtgctgcagg cggccgcccg cgccaaggac aatggctcca
     8521 gccgcttttg catgggcgct gcctggcgca accccaaggc gcgcgacatg cccgccatca
     8581 tcgatatggt gaagggcgtg cgcgccatgg ggctggaaac ctgcatgacg ctgggcatgc
     8641 tggagccgga gcaggccgac atgctggccg aggccgggct cgactattac aaccacaata
     8701 tcgacacctc gccggagcgc tataacgagg tgatcaccac ccgcaccatg gaggaccggc
     8761 tggacacgct gggcgaagtg cgccgggcgg ggatcaacgt gtgctcgggc gggattgtcg
     8821 gcatgggcga gacgcgggcc gaccgggtgg gctttgtcca cacgctggca accttgccca
     8881 gccatccgca aagcgtgccg atcaatgcgc tggtgccggt gaagggcacc gtgctgggcg
     8941 acatgctggc cgatacgccg ctggccaaga tcgacgatgt ggagtttgtg cgcacggtgg
     9001 cggcagcgcg cattgccatg ccgggcagca tggtgcgcct gagcgcaggg cgcgaaagca
     9061 tgagcgagat gacgcaggcg atgtgtttcc tggccggcgc caattccatt ttcaccggcg
     9121 acacgctgct gaccgcgccc aatgcgggtg acgacaaaga tgcggcgatg ttcgcccgcc
     9181 ttggcatcaa ggcaatggcg gtgaccagga ccgaggccga gcttgcggca gagcgcatgc
     9241 ccaggggctg tgcgaagctg gagcaggcgg ccgaatagtt tcatgcgcct cccggcgggg
     9301 ggcggatcgg gaagggtagt gtacaggtgt tcaagaaaat cctcattgcc aatcgtggtg
     9361 aaatcgcctg ccgggtgatc aagaccgctc gccgcatggg tatcgccacg gtggctgtct
     9421 attccgatgc cgatgcgcgt gcgcctttcg tgcggatggc cgatgaggcg gtgcatatcg
     9481 gcccgtcgcc cgccgcgcag agctacctca tcgccgacaa gatcatcgct gcctgcaagg
     9541 ccaccggcgc cgaggcggtg catccgggct atggcttcct gtcggagcgc accagctttg
     9601 ccgaggcgct ggccaaggaa aacatcgcct tcatcgggcc gccggtgggc gcgatcgccg
     9661 ccatgggcga caagatcgaa tcgaagaagc tggccaagag cgccggcgtc aacgtggtgc
     9721 ccggctttgt cggcgagatc cgcgataccg accatgcggt cgagatttcg gacgagatcg
     9781 gctatccggt gatgatgaag gcctcggccg gcggcggcgg caagggcatg cgcctggcct
     9841 tctccgagaa agacgtgcgc gagggctttg aaagcgttaa gcgcgagggc ctgaacagct
     9901 ttggcgatga ccgcgtgttc atcgagaagt tcattcagga tccgcgccac atcgagatcc
     9961 agatcctggg cgaccagcac ggcaacatcc tctacctgaa cgagcgcgag tgctcgatcc
    10021 agcggcgcca ccagaaggtg gtggaagagg cgccttcgcc cttcgtgtcg gcagcgatgc
    10081 gcaaggccat gggcgagcag tgcgtggcgc tgagccgcgc ggtgggctac tacagcgcgg
    10141 gcacggtgga gctgatcgtc agcggcgccg acaagaccgg cgagagcttc tacttcctgg
    10201 aaatgaacac ccgcctgcag gtggagcacc cggtgaccga ggcgatcacc ggcatcgacc
    10261 ttgtggaaca gatgatccgc gtggccgctg gcgagaagct ggccttcacc caggatgata
    10321 tcaagatcga tggctgggcg atcgagaacc gcgtctatgc cgaagatccc tatcgcggct
    10381 tcctgccctc cacggggcgg ctggtgcgct atcagccgcc ggtggaaggc tggagcgatg
    10441 atggcgccga gaacgccaat aatctgaatg gccggcgcgg catcgatggc gtgcgggtgg
    10501 acgatggcgt ctatgaaggc ggcgaagtca gcatgttcta cgacccgatg atcgccaagc
    10561 tgatcacctg gggcaagacg cgcgacgagg cggccgacaa gcagattgcc gcgctcgatg
    10621 ccttcgagat cgaggggctg ggccataaca tcgatttcgt ctcggcgatc atgcagcacc
    10681 cgcgcttccg ctcgggcgag ctgaccaccg gcttcatcgc cgaggaatat cccgaaggct
    10741 tccatggcgc ggccacggcc gaggaactga agcgcaagct ggcggcggtg gcgggttttg
    10801 tcgccacggc gcgggctgat cgggcgcggc ggatcgaggg gcagctggat ggcctgctgc
    10861 cgccgcctgc cggctggagc gtgcaggtgg ccggccagcg cttcgctgtc gaggtggacg
    10921 aggatgcggt gagcgttgac ggcgccgagg tggacctcgt catggaatac acgccgggtg
    10981 accggctgat cgccgccgag gtggatggcg aggcgctgac gatcaaggtg gcggccaccc
    11041 gcacggggct gaagttcacc acgcgcggcg cgatccatgt gatcgacatc ctgcccgatc
    11101 atgtggccca tctggccaat cacatgatcg agaagacgcc gccggatctt tccaaattcc
    11161 tcatctgccc gatgccgggc ctgctggtgg cgctgcatgt ggccgagggc gacaaggtgg
    11221 aagccgggca gccgctggcc gtggtggaag cgatgaagat ggagaacatc ctgcgcgccg
    11281 agaagagcgc gacggtgaag aagctgaacg ccaaggccgg cgagagcctg gccgtggacg
    11341 cgatcatcct cgagctggaa taaagcgggg agcggcaggg ggaagcattt tccccctgcc
    11401 ggcctgccgg tttgggctca gccggccagt tcgtcagtga ggaaggccgt cacttcggcc
    11461 agggtgacat ccttggtgag gaaggtctcg ccaatgccgc gcagcagcag gaaggccagg
    11521 cggccggcat ccaccttctt gtcgtgcagc atgtgggcgg ccagatccgc gcccgagaca
    11581 ttgaggccca gcgcgcgcag atccgccggc aggccgacag cggcgatatg ggcggcgatg
    11641 cgttctgcca cttcggccgc gatcagcccg cgccgggccg agaaacgcgc ggccagcacc
    11701 atgcccagcg ccaccccttc accatggagc aggcggctgg aaaagccggt ttcggcctcc
    11761 agcgcatggc cgaaggtgtg gccgaggttg aggagggcgc gccgcccggt cgtctcgaac
    11821 tcgtcttccg ccacgatgcg ggccttggcc gctaccgaat gggccacggc atgggcttgt
    11881 gccgccacat cgccggccag caccttggcg ccgttttcct cgcaccaggc gaagaagggg
    11941 gcatcgtcga tcaggccata tttcaccact tcggcatagc ccgcgcccag ttcgcgcgag
    12001 gggagcgtgg ccagcgcatc gagatcggcc agcaccagcg cgggctggtg aaaggcgccg
    12061 accaggttct tgccggccgc ggcattgatc gccgtcttgc cgccgacgga ggaatcgacc
    12121 tgcgccagca gggtagtggg cagctggatg aaatggcagc cgcgcttcag catcgaggcg
    12181 gcaaagccgg tgagatcgcc gataacgccg ccgcccagcg ccaggatatg atcgccccgc
    12241 tccacctcat gggccagcag ccattccacc gtgccgatga gattgtccca gttcttggtc
    12301 gcctcgcccg ccggcaggat gcgccacagc gcctgcttgc cggccgccgc cagcgaggct
    12361 tccaccaccg cgccccaatg ggcatgcacg cgctcgtcgg tcacgatcgg cacctgggcc
    12421 ttgcgcaggc gggtgccgca ctggccggcc agatccgcca gcaggccgcg gcccacgcgc
    12481 acctcatagg ggcggccggc aatgtcgacg gggatggtgt gcatggcttg ggctttcaat
    12541 caggaatggg cgttcaggaa ccggtggtgg cgggctgtgc cggcagccat ttgtcgaagg
    12601 cggcgaacag gcgcgagagc gtcttggcgt ggggggcgtg gccgctcatc acgcggatcg
    12661 gcgcttcggc gtagaagggc gcgcgctggt ccttcagctg gccgaggata tcgcgcgggt
    12721 tgccctggcg cagcagcggg cggttgtcct tgcgggcggt gcgttccacc agcgtgtcga
    12781 gatcggcatc gagccagacg gcgatcgcct tgtcgagaat cagcgcgcgg gtttcggtgt
    12841 tgcagaaagc gccgccgccg gtggcgatca ccttcacgcc tttggagggg gcatgggcgg
    12901 gcagcggcag gcgatgatcg ctcgccccca catcggcgcc cagcaggcgg gcgatcaccc
    12961 ggcgctcgcc atcgcggaag aaagcctcgc catggcgttc gaaaatctcg ggaatcgaca
    13021 tttgcgcggc cttctcgatc tcctcgtcgg catcgagaaa gggggcgccc agttgcgcgg
    13081 ccagcttgcg gccgaggctg gatttgccca cccccatcat gcccaccagc accacagggc
    13141 gggtgatgcg ctgggcgatg agggacatct cgtcggggcc gggcagggga tgttcgtggt
    13201 ccattgcagc cacgcctata cttttgctaa gcggcggcgc aagcatgagg ctgtgatcag
    13261 gcgttgtttc ccgcagcaat gcgcccatgc aaacttcgct gtgaagggtg acgcgtgacg
    13321 atcaggatca aacggtatcc caaaagcacc tggcccacag gcttcaggca gggggcgcag
    13381 cggcgcccca ggctggccgc ggggctgggt gtggtgctgg cgctggccgg ttcgctgatg
    13441 tggatggcat ggcgcgctgg cggccctgtt gcccgccacg aggtggtgct ggcactgccg
    13501 gccggcgagg tggggcggtg agcggcctgc gtcgcctgtt gctgggggcg gggctgctgg
    13561 cggggctggc gggtgcggcg ctggcccagc aggggccgga atcgctgctg ccacccggtt
    13621 tcgatcggcc tgcgcggcct tcccgcagcc cgcagccgcc ggcgccggcg cgttccgcag
    13681 cgccggtggc gatggccgcg cccgtgatcc agccgcaaac cctgtttggc gggccacccg
    13741 cgccgccgcc cggcgccttg ctgggcgcgg tgccgctgcc tgccggggtc accagtttca
    13801 accagttgat ggccctgccg caggaccaga tcgaccagtt gctgggcctg accaccaagg
    13861 acgacatccc cgccggcgcc cgccatgcca tgcgccaggt ggggctgatg aaccaggccg
    13921 aaggcgggct gccgccgttc tcgctggacc atcaggatgc cgggctggtg cgcgcggcgc
    13981 tggcgggcaa caatggccat atggtgtcgc gctgggggca tattctgctg cggcgggcgc
    14041 tggcctcgcg gctggatgcg ccggaggggc tg
//
LOCUS       DASRYE010000148         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_149645,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000148 DASRYE010000000
VERSION     DASRYE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149645"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFF98_07940"
     CDS             <1..244
                     /locus_tag="VFF98_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZU63599.1"
                     /translation="EAAQFLKDEAKRGRKKPMVGFIAGRTAPPGRRMGHAGAIVSGGQ
                     GGAEDKIAAMEEAGIRVSPSPSLLGETLAELLKEIA"
     gene            313..1317
                     /locus_tag="VFF98_07945"
     CDS             313..1317
                     /locus_tag="VFF98_07945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU63600.1"
                     /translation="MAHSLPRLSLLDLVPVIEGGSVGQALANAADLAAHAEALGYHRY
                     WVAEHHGMASVGGAATSVVLAHLGAHTRRIRLGAGGIMLPNHAPLQIAEQFGTLDALH
                     PGRIDLALGRAPGSDGRVAMALRHGDPHAFPQQVIDLQDYFAGDPRMGFTATPGAGAQ
                     PQMWILGSSTYGAELAAALGLPYGFASHFAPAMLDEALATYRRLFRPSAWLERPHVMV
                     GFNVFAADTLAEARFHATSMQQRFVALRTGGANAPFQPPVEGYYESLPAPHRAMLDRM
                     LECTAIGTEADVAAGLAALVARTQADEVIIGGQIFDHGARRRSFALAAAALQGLPNPA
                     "
     gene            1415..2170
                     /locus_tag="VFF98_07950"
     CDS             1415..2170
                     /locus_tag="VFF98_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZU63601.1"
                     /translation="MFAPSPAAPSPASIAALFDVDALALVTSFICLATAFVLGTLIGL
                     ERQWRQRTAGLRTNVLVAVGAAAFTDLGLRVAGVDGGVRVISYVVSGIGFLGAGVIMK
                     EGTHVRGLNTAATLWASAAVGSFAGARKPAEAVLVAVFVLAGNTLLRPLVDFVNRRPI
                     NPGETEALYRVHVVCPADLVSQARDLLFDELETHHYPIREMETLAESEAHVELAAILV
                     PTTANPADLDAITAHIARHEGITSATWTVSATN"
BASE COUNT          329 a          803 c          791 g          372 t
ORIGIN      
        1 agaggccgcg cagttcctga aggatgaagc caagcgtggc cgcaagaagc cgatggtggg
       61 cttcattgcc gggcgcacgg cgcctccggg ccgccgcatg ggccatgccg gcgcgatcgt
      121 ctcgggcggc cagggcggcg ccgaagacaa gatcgcggcg atggaagaag cgggcatccg
      181 cgtctccccc tcgcccagcc tgctcggcga aaccctcgcc gaactgctga aagaaatcgc
      241 ctgatactgt gatacaccgg ccggcggcag cggccccttg cgctgctgct gccggcaccc
      301 cacatgggcg gcatggccca ttcccttccc cgcctgtcgc tgctcgatct ggtccccgtg
      361 atcgaggggg gcagcgtggg gcaggcgctg gccaatgcgg cagatctcgc cgcccatgcc
      421 gaggcgctgg gctatcaccg ctattgggtg gcagaacacc atggcatggc cagcgtgggc
      481 ggtgcggcca catcggtggt gctggcccat ctgggcgccc acacgcgccg catccggctg
      541 ggggcgggcg gcatcatgct gcccaaccac gccccgctgc agatcgccga gcagttcggc
      601 acgcttgatg ctctgcaccc gggccgcatc gatctggcgc tgggccgcgc gccgggatca
      661 gacgggcggg tggcgatggc cctgcgccat ggcgatccgc acgcctttcc gcagcaggtg
      721 atagacctgc aggactattt cgccggcgat ccgcgcatgg gcttcaccgc cacgccgggc
      781 gcgggcgcgc agccgcagat gtggattttg ggctccagca cctatggcgc ggaactggca
      841 gcggccttgg gcctgcccta tggctttgcc tcgcattttg ccccggccat gctggatgag
      901 gcgctggcca cctatcgccg cctgttccgc ccctcggcct ggctggagcg gccgcatgtg
      961 atggtgggct tcaacgtctt tgccgccgac acgctggccg aggcgcgctt tcatgccaca
     1021 tcgatgcagc agcgcttcgt ggcgctgcgc acgggcggcg ccaatgcccc cttccagccg
     1081 ccggtggaag gctattacga aagcctgccc gccccccacc gcgccatgct cgatcgcatg
     1141 ctggaatgca ccgctatcgg caccgaggcc gatgtggccg ccgggctggc ggcgctggtg
     1201 gcgcgcacgc aggccgatga agtgatcatc ggcgggcaga tcttcgacca tggcgcgcgc
     1261 cggcgcagct ttgccctggc cgccgccgcc ctgcagggcc tgcccaaccc cgcctgaggc
     1321 cccccctgag gccccctttg cgaccatagt tggcaggtgc aatcggccag cggctgtgcc
     1381 atgaggcgcg cgcccgttgc ccacaggatt tgccatgttc gcgccttctc ccgccgcgcc
     1441 ttccccggcc tcgatcgccg cgctgttcga tgtcgatgcg ctggcgctgg tcaccagctt
     1501 catttgcctt gccaccgctt tcgtgctggg cacgctgatc gggctggagc ggcagtggcg
     1561 ccagcgcacg gcgggcctgc gcaccaatgt gctggtggcg gtgggggcgg cggccttcac
     1621 ggatctgggc ctgcgcgtgg ccggggtgga tggcggcgtg cgcgtcattt cctatgtcgt
     1681 ctcggggatc ggctttctgg gcgcgggcgt gatcatgaag gagggcaccc atgtgcgcgg
     1741 gctgaacacg gcggccacgc tgtgggcttc agccgcggtg ggcagctttg ccggcgcgcg
     1801 caagcccgca gaggccgtgc tggtggccgt cttcgtgctg gcgggcaaca ccttgctgcg
     1861 cccgctggtc gatttcgtca accgccggcc gatcaacccc ggcgagaccg aggcgctcta
     1921 ccgggtgcat gtcgtatgcc cggccgatct cgtgtcccaa gcgcgcgatc tgctgttcga
     1981 cgagctggaa acccaccatt accccatccg cgagatggaa acgctggcgg aaagcgaggc
     2041 ccatgtggaa ctggccgcga tcctcgtgcc gaccaccgcc aacccggccg atcttgatgc
     2101 catcaccgcc catatcgccc gccacgaagg catcaccagc gcgacatgga cggtgagcgc
     2161 caccaactga gcaccaacaa agcccccttc cggccgggca ggccgaaagg gggcaggggt
     2221 ccagtcggag ggtatggggg actggaagat cagtcggcgc gggcggccgc cttgccgaaa
     2281 ctgtaacgca cgcca
//
LOCUS       DASRYE010000149        33221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_24104,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000149 DASRYE010000000
VERSION     DASRYE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 33221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33221
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24104"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..797
                     /gene="pyrF"
                     /locus_tag="VFF98_07955"
     CDS             102..797
                     /gene="pyrF"
                     /locus_tag="VFF98_07955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZU63602.1"
                     /translation="MNHNPVYLALDLPRLDAAEALARRVKDHVGGIKLGLEFFCAHGH
                     HGVHDLARVGLPIFLDLKLHDIPNTVASAMQAINVLQPAIVTIHAAGGRAMMEDAKAA
                     AGEHTKVVAVTVLTSLDGGDLASIGVAAQPHEQALRLAELAHSAGLDGIVCSGHEVGA
                     VHRQWADGFFVVPGLRPAEHHHAANGAAGDQKRIVTPRAARDAGASVLVIGRPISRAE
                     DPLAAARAIEATL"
     gene            873..1613
                     /locus_tag="VFF98_07960"
     CDS             873..1613
                     /locus_tag="VFF98_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014809.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZU63603.1"
                     /translation="MPFAPLPATAAIAFIAAALIGTPALAAAPAELPAGHTLLTLSAQ
                     GQSARQPDIAGFSAGVATAGRNAAEAMAANAAAMSRLIAALKAAGIEPRDIQTSRLAL
                     TPTYAERRPGDTRAPAITGYQAANTVAVRERHVAEAGRVIDALVAAGANDISGPDFTL
                     ADPKAAEDEARADAIAQLLARARLYAQAVGLKVAQVRAISEFVPSQPMPMARSMALMA
                     MPAAATPVEAGEVESQIRVTMEFDLGPA"
     gene            1689..2330
                     /locus_tag="VFF98_07965"
     CDS             1689..2330
                     /locus_tag="VFF98_07965"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZU63604.1"
                     /translation="MSDTGPAIKICGITTSAALAAAITARAHYAGFVFFAKSPRNLAL
                     ADCGPLRDQAAGQIGRVGLFVDAGDDAMAEAIAAAGLDVLQLHGSESPERVAQVKARF
                     GLPVWKALPVAAPADVARADAYARIADLLLFDAKTPKGALPGGMGLAFDWQLVKGFRA
                     APWGLAGGLSPANVAEAVAVSGAPLVDCSSGVESAPGVKDPALIAAFCRAARG"
     gene            complement(2430..2882)
                     /locus_tag="VFF98_07970"
     CDS             complement(2430..2882)
                     /locus_tag="VFF98_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63605.1"
                     /translation="MRLILISAAAAALAATPAMAGETHVDVHTGVGWNDGQSAKADVG
                     IGVGYDFNISSNAFMGLEEDMDRVQADGAKLRFSTVGRLGTAIGNGNKVYALGGAVYG
                     LGADGSVVGAGIEHSFGPAFAKLEYRHQFLDDGNTDANAALIGVGFKF"
     gene            3092..4312
                     /gene="trpB"
                     /locus_tag="VFF98_07975"
     CDS             3092..4312
                     /gene="trpB"
                     /locus_tag="VFF98_07975"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZU63606.1"
                     /translation="MTNHTAPANSLRNQPDERGHFGQFGGRYVAETLMPLILDLEREY
                     TAAKADPAFHADFEDLLTHYVGRPSPLYFAPRLTEELGGAQVWFKRDELNHTGAHKIN
                     NCIGQILLAIRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGATDVARQAPNVF
                     RMKLLGAEVVPVTSGAGTLKDAMNEALRDWVANVHDTFYIIGTAAGPHPYPELVRDFQ
                     SVIGKEARAQMLERTGRLPDLLVAAIGGGSNAIGLFHPFLDDASVKMLGVEAAGYGLD
                     KDHAASLAGGRPGILHGNKTFLLQDEDGQILEGHSISAGLDYPGIGPEHAWLKEVGRV
                     DYTSVTDEEALGGFQLLCRTEGIIPALEPAHAIAAVARVAPTMPRDSIILANLCGRGD
                     KDIFTVAKHLGIDL"
     gene            4309..5109
                     /gene="trpA"
                     /locus_tag="VFF98_07980"
     CDS             4309..5109
                     /gene="trpA"
                     /locus_tag="VFF98_07980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZU63607.1"
                     /translation="MSRLASAFAKGRPALVTFVTGGDPTPQATPAILDALVAGGADVI
                     ELGMPFTDPMADGPAIQQANLRSLGAGTKTADILRIAADFRARHADVPLVLMGYANPM
                     ITRGAEWFASACAEAGVDGIICVDIPSEEDPELGPALRAAGIAFIRLATPTTDTARLP
                     AVLQGAGGFLYYVSVAGITGKQQAQLASIEEAVARLKAATDLPIAVGFGVRTPEQAAA
                     IAGHGDGVVVGSALVDLVAAHGADAAPHVQALVASLAQAVHSARKISA"
     gene            5106..5966
                     /gene="accD"
                     /locus_tag="VFF98_07985"
     CDS             5106..5966
                     /gene="accD"
                     /locus_tag="VFF98_07985"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZU63608.1"
                     /translation="MSWLDKVRNSLTSLANRRAEDATPDNLWTKCPHCGAMLFTKEYE
                     ANLSVCPQCDHHGRIGTDARLSHLLDPDYQLLTSPRVKEDPLKFRDSKRYTDRLKAAR
                     AASPHIDALTNALGAIKGHKAVVGVQDFHFMAGSMGMAVGAAFVAGAQRALTEKCAYI
                     ICTAAGGARMQEGILSLMQMPKTTVAVRRLNAAGLPYIVVLTDPTTGGVTASYAMLGD
                     IHIAEPGCLIGFAGQRVIQNTIREKLPEGFQRAEYLHEHGMVDMVVKRHDLRDTLASL
                     LSYLSPAKAA"
     gene            5987..7306
                     /locus_tag="VFF98_07990"
     CDS             5987..7306
                     /locus_tag="VFF98_07990"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZU63609.1"
                     /translation="MKDFATHDHPAVAAQLARLSALSVPQGRLGLEVITGLLAALGNP
                     ETRLPPTFHVAGTNGKGSTCAYLTAMLEAAGHRVHSATKPHLVRYNERIRLGGRLIGD
                     GHLAALLAEVLDAAATLPEELSPSFFEVTTAAIFLAFSREPADACVIEVGLGGRLDAT
                     NVIPAPLACGIATLGIDHEAFLLRPEAGAPEGPLARIAWEKAGIARPGRPLVTQAYPE
                     EAAEEVRRVAAAAGAPLLMRGESWEAAVGEAIHYRDADGLLTLPLPTLAGRHQADNAA
                     LAIAMLRAQSVLTVSEAAMAEGIRAARWPARLQLLGQGPLTELAPGRKLWLDGGHNPD
                     AGAAIARHFADHPPLHLIIGMLANKDPAAIVRPLAGQLLSLSVVPAPGHDAHAPEAFA
                     GLTDLAIAPRADVAEALAALATPGDVLICGSLYLAGEVLRLNQEFPD"
     gene            complement(7288..8961)
                     /locus_tag="VFF98_07995"
     CDS             complement(7288..8961)
                     /locus_tag="VFF98_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63610.1"
                     /translation="MAEPSSQQPPRGLRLLATALSGAKPAAMLGYGFASGLPFALLIG
                     TLNAWLGEVGVKLATIGVFSWIGLCYSFAFLWAPVVDRLRLPLLARLGRRKSWIIPCQ
                     AVLVAGLVVLAQLDPKAAIGPFAAVAFLCALASATQDTAINAWRIDVADAATSVELLS
                     AIYQFGYRTASIVGGAFALLMAAHMRWPQVFLAMAAMLGAVALACLAAPDTPARTPAT
                     DEAGTQQAPRGQALLLALVLASWGWAVASLVGFMAQMLAPARHHHGAPVSVAAFTRGT
                     GPWIVVATVLVPLAVAALAHALRRRGAVRQVRMAGLWGAARHHLYGALVAPLEDLVAR
                     RGIWVLSLIGFILSYALCYNIWASFAYPFYLDALHYTKEEVAFASKIFGIFMTMIGIS
                     LGGLLFARAGRFATMVVGAVLPPLGNLLYADLADGGRMIDAFAHLLRLDGLAASLGAG
                     PALLRLLVAICYENISTGLALTAFVAYVSGVVNKRYTAIQYALLSSLVSLVGTLGRGI
                     AGEAFDHYGYAPVFRWTALAGLVSVAFVGLEWLGARARPFHAEDQSGNS"
     gene            9104..10171
                     /locus_tag="VFF98_08000"
     CDS             9104..10171
                     /locus_tag="VFF98_08000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZU63611.1"
                     /translation="MAKPPSRTPSSRSTRPGGERRPSGERRSPGERGPGGERRASGER
                     GAANERGASNERSIERGRPIPPPRQRRPTPAWAKPAPAPANPMPRGEGEERIAKLLAR
                     AGIASRREVERMIAEGRVKRGEEVIATPATLLRSLKGITVDGRAVSAPEPTRLFAFHK
                     PSGLLTAERDPAGRPTIYTALRNALPAGTPRLMPVGRLDLNTEGLLLLTNDGELKRAL
                     ELPSSGVTRTYRARTFGDITQDRLEELMEGIEIDGVRYGSIDANMERRTGRNQWVELT
                     LTEGKNREVRRVLEHLGLQVSRLLRVAYGPFHLGDLPRGQAVAIRQIEVERLRKSLRS
                     GKGANTPAAIPADQTPQAKDN"
     gene            10171..10737
                     /gene="rsmD"
                     /locus_tag="VFF98_08005"
     CDS             10171..10737
                     /gene="rsmD"
                     /locus_tag="VFF98_08005"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZU63612.1"
                     /translation="MRIVAGEWRGRKLAAPDGDATRPTADRTRETLFSMLLSRLGDFE
                     GLRVADLFAGSGALGLEALSRGAAHALFAEQDASALRAIRANIANLRAQAQCDVRAGS
                     VLALGPVKQPYDLLFLDPPYHSGAGAVALDKLKRLGWIGPASWIALETEAREEPKLRG
                     LEAVAERTVGKARITLLRLPASAESRLG"
     gene            complement(10734..12029)
                     /locus_tag="VFF98_08010"
     CDS             complement(10734..12029)
                     /locus_tag="VFF98_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63613.1"
                     /translation="MNQPPTSLLSPLRQHRRILAAALVGTTVEFYDFYVFATATALVF
                     GPLFFPAASPAAQGIYSFMAFGIAFVARPIGAVLFGHFGDRVGRKSTLVASLLLMGGC
                     TFAIALLPAYASAGWLAPALLCTLRFGQGFGLGGEWGGAALLAVEYAPEGWHARFGAA
                     PQLGAPIGFLAANGLFLILGVCLTPAEFLAWGWRVPFLLSAVLVGLGLWVRLRIAETP
                     AFADAIARKTRVRVPLAYLLRRHFGVLMAGSAGVVACFALFYLATAFALSLGTGALGY
                     NRTDFLLVQLGANLFFAVGIVLAAIWADKTRPGEVLGWGSVLTVLVGLAFGPGLLSGS
                     LVWVFVTLSAALLVMGLVYGPLGGWLPTLFPVPLRYTGISLAFNLGGILGGALSPLAA
                     EVLARGGWGAAVGLILAVAGLLALAGVSLARPAPVPAAA"
     gene            12227..14530
                     /locus_tag="VFF98_08015"
     CDS             12227..14530
                     /locus_tag="VFF98_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZU63614.1"
                     /translation="MDDTTLPANAPEWLSRLNTPQREAVLASEGPVLMLAGAGTGKTA
                     ALTARLAWLIACRKAWPSEILCVTFTNKAAREMRERVGHLIGDAVEGMPWLGTFHAIA
                     AKMLRRHAELVGLQANFTILDTDDQLRLLKQLIQAEGLDEKRWPARQLASLIDRWKNK
                     GLGPDELDALENEAYANGKGAHFYRLYQARLITLNACDFGDLLLHMLAILKRHRDVLE
                     MYQARFKYIMVDEYQDTNQVQYLWLRLLAQGVEGRRHNICVVGDDDQSIYSWRGAEVA
                     NILKFEKDFPGARVIRLEQNYRSTPHILGAAAGLIRENAERLGKELWTEATGGDKLQV
                     IGVWDAPEEARRVGEEIERLEREGLPLTHIAILVRAQFQTREFEDRFISIGLAYRILG
                     GFRFYERAEIRDALAYLRLVAQPSDDLAFERIWNTPKRGLGDKSMEKLHRFARAANLP
                     LLAAAIAIADTDELPARARGTLLALARDFARWRDLAASESPANLARTILDESGYTAAL
                     QVEKSAESAGRLENLVELARAMEEYESLADFLEHVSLVMDNDANDDAEKVTIMTIHGA
                     KGLEFDHVFLPGWEEGVFPSQRSLDEGGLASLEEERRLAYVAITRARRHCTILHAANR
                     RIYGQWTSSLPSRFIGELPKAHIMEESSLSGGASLWRAQWSERADPFAHVAQERSARA
                     ETRGPGWQRASQQPFTPTPRRIAEPTRSAASFAAKARLDIRPGDRVVHDKFGTGTVAA
                     QEGNKLEIDFDSAGRKRVIDSFVRPAS"
     gene            complement(14594..16105)
                     /locus_tag="VFF98_08020"
     CDS             complement(14594..16105)
                     /locus_tag="VFF98_08020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZU63615.1"
                     /translation="MSFADLGLSEELLQSVAAAGYDTPTPIQAQAIPAVLMMRDLIGI
                     AQTGTGKTASFVLPMIDILAHGRRRALMPRSLILEPTRELAAQVAENFEKYGKNHDLK
                     MALLIGGVQMGDQVKALSDGVDVLIATPGRLMDLFERGKILLTGCNLLVIDEADRMLD
                     MGFIPDIENICTKLPAQRQTLLFSATMPPPIKKLADRFLTNPKYIEVARPATTNTNIT
                     QHKVKVAAKRKREMLRHLLQVDNVTTAIVFANKKTTVRDLAKSLKRHGFAAGEIHGDM
                     DQPARLAELQRFKDGQVNILCASDVAARGLDVKGVSHVFNFDTPWHPDDYVHRIGRTG
                     RGGATGRAFTLFTSEDAEAIASVEKLTGGPIPEMVVELGGVDEGDDAPAPRSAARGRK
                     AAPHDRRRPDRQAAPEPEVEAEKPARAEKPARAEKPVRAEKPARAEKPARAARPERAA
                     RPERDERRDARSDERRADRREDRRPRWRDDDGPTRPGEWNGPIPEFLGISALH"
     gene            complement(16235..16816)
                     /locus_tag="VFF98_08025"
     CDS             complement(16235..16816)
                     /locus_tag="VFF98_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63616.1"
                     /translation="MAGAALVLSGGLPASLRAEGLRLAMALPEPLTGGLRALRAAIPD
                     QPDRADLPEPPGLPDETAAQVRVEQHFSIRITPGSPAMPPFVLEEAQQEERPAPTPER
                     DIGRCVAMSNILGVQSGEGNRLLLFMRDQRIISAVLERTCRAHDFYSGFYMQRSPDGM
                     ICTGRDKLQSRTGMNCRLKALRMLITVPGRRFP"
     gene            17078..18505
                     /locus_tag="VFF98_08030"
     CDS             17078..18505
                     /locus_tag="VFF98_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU63617.1"
                     /translation="MTDTAFLAAAAALLGPRGLATDADTLAPWLTDWRGRYTGAALAL
                     ASPATTAQVAALVQLCAAHGVAIVPQGGNSGMSGGATPDASGRALLLSLRRLNGIGPV
                     DRAARRVTCGAGVILQHLHEAAEAAGLRFPLSLGGKGSATVGGLVSTNAGGTQVLRHG
                     NMSAQVLGLEAVLADGTVWSALTPLKKDNRGPDLKQLFIGAEGTLGIVTAATLKLAPA
                     IADRRVVWCGVASLQQARALMLHAEAALGEALEGFEVLPHSSLEAVEAYLPDVRRPLA
                     APRPWYALIEVVADTASAASLGERVEAVLASAFEAGLLDDAVIAANETQAEAFWTIRE
                     SISPAERAKGPAMQHDISVPVEAMPDFVDAAISAMKARFAGTEAIAFGHLGDGNVHFH
                     VIAPRGVERAAWEAGEGKAISAAVHDLVTQWGGSISAEHGIGQLKVAELGRLADPARL
                     KLLRALKAALDPAGLLNPGKLVPPV"
     gene            18658..19425
                     /locus_tag="VFF98_08035"
     CDS             18658..19425
                     /locus_tag="VFF98_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HZU63618.1"
                     /translation="MASAPQNASLPVFYKDLVPLNSREHASWKSRTTDRATWLVGQHA
                     VPLTVEEFPQAQRHFPIVFSVGDNPVPLALMGLNEGINVFVDDEGKVADNIYLPAYAR
                     RYPFMLARLTPDAQELSLCFDPNSDLIGEGEEGTPLFDGEEPSQTTKEMLGFCEQFEQ
                     AGARTAAFVQELIEHKLLIDGEVAIRQEGNEQPFIYRGFLMVDQNKLREVRGDVLRKW
                     NQSGLLPLVIAHLFSLDLMREIFARQAQLGKGPLVAA"
     gene            complement(19817..20836)
                     /locus_tag="VFF98_08040"
     CDS             complement(19817..20836)
                     /locus_tag="VFF98_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZU63619.1"
                     /translation="MACGFDRKGALGQPCAMTDGPQTDRAAQADSPLSGGAIPGLAGA
                     PAFVVPCGADGARLPVLVAVPHAGRRYPPELLARLRHPEQAALKLEDRLADELGRALA
                     EATGAALLVAEAPRAMIDLNRHPADVDWTMFAGAAPQSRLAPPAGGRAAGGLGLVPRR
                     LPGLGELWQGRPPVAEVEARIASVHAPWHACVAAELAALQARWGAALLVDLHSMPPLP
                     AAPGAPRTQVVVGDRFGASCGGMLVAQAFSALAGAGWLAAHNRPYAGGYTLDRHGMPA
                     RGRHAVQLEICRTTYLDAQLVAPGPGFAACVAMLCALVRALGEATAAMGSAQLAGGSC
                     AQAAE"
     gene            20959..21333
                     /locus_tag="VFF98_08045"
     CDS             20959..21333
                     /locus_tag="VFF98_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU63620.1"
                     /translation="MTRILLAEDETAMRTYLARALEKAGYDVVAVDRGTAALPLLESQ
                     HFDLLLSDIVMPEMDGIELAQRCGEISPATKVIFITGFAAVTLKANREAPQARVLSKP
                     FHLKDLVLEVQRVFGHSSEVSV"
     gene            21458..21532
                     /locus_tag="VFF98_08050"
     tRNA            21458..21532
                     /locus_tag="VFF98_08050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21490..21492,aa:Val,seq:gac)
     gene            complement(21521..22252)
                     /locus_tag="VFF98_08055"
     CDS             complement(21521..22252)
                     /locus_tag="VFF98_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HZU63621.1"
                     /translation="MADFGHVAAVFGGRATADLLQNLVLVLDLGGTFAFALSGAMAGV
                     RRRLDIFGVLVLAFAASTFGGITRDVLIGAIPPASLQDWRYLAVSVVAGLSCFFWFAG
                     MEKWRNPVLVLDAAGLALFAVSGTEKALAFGLPPLMAALLGMVTGIGGGVARDLFLSD
                     IPRVLRSDLYAVAALAGAAVVVIGSALHWPVIPSALGGACCALACGWRRSAGAGICPW
                     PARAIRRHEKTRLGFWPGGFFEWWA"
     gene            complement(22252..23520)
                     /locus_tag="VFF98_08060"
     CDS             complement(22252..23520)
                     /locus_tag="VFF98_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU63622.1"
                     /translation="MSTDQTLALKRLLAPLPDLLPALEAAYKDIHAHPELSMQEQRTA
                     ALAARHLADEGYEVTTGIGQTGVVGLMRNGEGPTVMLRADMDALPILEDTGLAYASTA
                     TATDPAGKAVPVAHACGHDMHVAWLMGASALFARTRDAWKGTLIVLFQPGEETAQGAR
                     AMIDDGLFEAFPKPDIVLGQHVMVGAAGTVAGSAGPITSAADSFEIRLFGRGAHGSMP
                     QASVDPVVMAASVVMRLQGIVSREVAATEAAVVTVGVLQAGTKENVIPDEALIKLNVR
                     SFDAGVRSRVLAAIRRIVEAEAAASGAPRPPEITPLDSYPLNVNDRQASDRVAQAFRA
                     HFSTERVFHTGPAPASEDFGCFGTEWQAPSVFWFVGGTDPAAYAQAKAAGRLNDLPVN
                     HSPHFAPVLHPTLAIGVEAMAAAALAWNAA"
     gene            23684..24367
                     /locus_tag="VFF98_08065"
     CDS             23684..24367
                     /locus_tag="VFF98_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZU63623.1"
                     /translation="MEDLFADRTIRDSRLRRPELAALGAPHRLIGCDLEEADLSGLDL
                     SGWVFEQCDLRRARLAGARCERSLWQSCRGAFADFTGCDLSEARFVACDFNNAMLRRS
                     TLEEARFLRCKCTGADFSEVKAITLHIEETLLIDARLAGLSFRKAQLVRVDFSRSDLR
                     KCDFRMTSFEACSLREAQTHGARFEQADLRGADLGGIGLGDAARFRGATISREQAAQL
                     LGELGLKVR"
     gene            complement(24390..24848)
                     /locus_tag="VFF98_08070"
     CDS             complement(24390..24848)
                     /locus_tag="VFF98_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZU63624.1"
                     /translation="MTITYEGPLEPGWIDYNGHLRDAYYALIFSYATDRLMELIGLDA
                     AGRKAQAATLYTLEAHLLYLAEVKGDAAVRVCTTLLAHDAKKIHLLHALHVAGRDGPA
                     AVGEQLLIHIDTASGRSAPMGEGLLAAVRALVAVAGDGAPYAGRAIALRR"
     gene            complement(24856..25791)
                     /locus_tag="VFF98_08075"
     CDS             complement(24856..25791)
                     /locus_tag="VFF98_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU63625.1"
                     /translation="MSEISHVAVLGAGVIGASWSALFLAAGCDVAVFDPAPDAEQMVR
                     AYIRDAWPTLLELGLAQAGRSGALSFHATAADAVRDADFVQESVPERLAIKHALYAEI
                     EPALKPGAVVASSASGLKLGEMQAGWGDPGRLILGHPFNPPHLIPLVEVMGNAQTAPG
                     VIELAERFYAAVGKVTIRVNREVPGHVANRLQAALWREAIHLVAIGAASVADVDTAVW
                     AGPGLRWAAMGPTMLFNLGGGPGGLAAFCEKYADSFHRWWEDLGDPRLDEATAAALVA
                     GVNEEAASASQAELAARRDALITAMLRATAALPRR"
     gene            complement(25791..26543)
                     /locus_tag="VFF98_08080"
     CDS             complement(25791..26543)
                     /locus_tag="VFF98_08080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63626.1"
                     /translation="MAEPQVVIVTGGSRGIGRAIVERFWAQGDHVLTCGRAERPADLP
                     AAVAWLRADVAQAADAERLVAEACHLFGPPTVLVNNAGIQIEKTLADTSDDDWDLLMG
                     ANARGPFNMCRAVLPLMAQAGGAIINIGSISGLAADPSMALYNASKAFLHGLTRAIAV
                     DHGPQVRCNAVCPGWIATAMADAPFATAADPARARADMLARHPAGRLGRAADVANMVA
                     WLASAEAGFVTGQCLVVDGGLTAACPIQPRLF"
     gene            complement(26536..27975)
                     /locus_tag="VFF98_08085"
     CDS             complement(26536..27975)
                     /locus_tag="VFF98_08085"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HZU63627.1"
                     /translation="MHTQLLIGGELVAGEGASTTVLDPATGETLAHLGEASAAQVEAA
                     VAAADAAFATFGQSTPAERAALLLDIAGQIEAHAGELAALESANVGKPWPSARDDEMP
                     LTIDTFRFFAGAARTMAGVAAGEYVAGHTSLIRRDPIGPVAAIAPWNYPLMMAAWKLA
                     APLAAGCPVVLKPSELTPLSTLRLARLLADVVPAGVVNILHGSGAGVGDQLINARGIE
                     AVSITGSPATGRAALRAASAQLRQVHLELGGKAPVIVYADADIDRLVATIRDGAYFNA
                     GQDCAQPCRLIVADAVYDRVVAGLAEAVAQIRVGPPRAAGTQMGPVISAAQRDRVAGF
                     VDRARGHAEVVVGGAAVTGPGFFYAPTLIADVDNHAEIATSEVFGPVVTVSRFGPDED
                     PVAIANAGRYGLASSVWTADAGRAMAATARLRYGFTWVNTHGVATPEMPWAAMKGSGH
                     GCDMSVFALDAFTAVRHVMIAHDVMIAHG"
     gene            complement(27996..28925)
                     /locus_tag="VFF98_08090"
     CDS             complement(27996..28925)
                     /locus_tag="VFF98_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZU63628.1"
                     /translation="MAKSVIITCAPTGGVHTPSMSPYLPITPDEIATASIEAAEAGAA
                     IIHLHARNPENGKPDPRPELFRQFMPEIARNSDAVLNVSTGGGLGMSREERLRAAVSV
                     SPEMASLNVGSMNFGIFPMGAKQTNWKHEWEPAFLESTRDFIFRNTFTDIEYVIKELG
                     EGHGTRFEFECYDLGHLYNLAWVIDQGWIKAPFFVQMVFGILGGVGADLDNLMHMHTI
                     AQKLFGDAYEWSVLAAGKHQMQFATQAALLGGNLRVGLEDSLYIGPGEKAKSNADQVR
                     KIRSIVENLGLTIATPAEARQRLGLKGGDKVSF"
     gene            complement(28975..30117)
                     /locus_tag="VFF98_08095"
     CDS             complement(28975..30117)
                     /locus_tag="VFF98_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HZU63629.1"
                     /translation="MHHVITANQFDHVAKGYSTDPLDTQSLRAEAYTQGRWFDIDQQE
                     IIAKSWQWVCHVEKLRDPGSYTTATIAGNPVAVVRGKDGVLRAFHNVCKHRAHPVLAG
                     EGKVSRIVCPYHAWCYGLDGSLAAAPQVGHLKNFSKADIHLEPVQVEEFCGFVFVNLD
                     LEARPLRETSGDLAQEILGWAPDIGQLTFGHRLTYTIRSNWKNVVDNFLECYHCPSSH
                     RDFVTLVDMDTYKVTTHGIYSSHMAEAGHGANSAYDTSAAKVRTHAVWWLWPNTCLMR
                     YPGRSSMIVLHIIPVGPDLTLETYDFFLETPEADAMELEAIRYLDEVLQAEDIAIVEN
                     VQRGMNSPAFDQGRIVADPAGSGLSEHALHHFHGLVLDAYRGAIAP"
     gene            complement(30244..31257)
                     /locus_tag="VFF98_08100"
     CDS             complement(30244..31257)
                     /locus_tag="VFF98_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZU63630.1"
                     /translation="MQRQPSRPMRMAIIVLPSFNMAATMAFIDPFRATNYLSGRQHFL
                     WDVVSAEGGEVMASNSLAITTSPLASLKGAEIDLAIVSSSWSPERHCTPALRHMLRGW
                     AQGGVTLGALDTGAFLLAEAGLMHQRRATCHYEHIDALAELYPDIDVREDMIVIDERI
                     MTCCGGSAAVDLALHIICQLHGATMANEAAHYLFHQSLRPCDTRQSPDIREPLGSAVP
                     PIVRRAIRLMEGHLEETIRIPDLCTQLGVSQRSLDRLFGDIVGKSPAAYYRDIRLDRA
                     RGLVTQTSLSLFEIGIASGFSNQSHFSRAYRLRFGVSPRQDRLEGRIPFEFRAWPMHR
                     HSA"
     gene            complement(31264..31914)
                     /locus_tag="VFF98_08105"
     CDS             complement(31264..31914)
                     /locus_tag="VFF98_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZU63631.1"
                     /translation="MNLMHVDSSPKGEISNSRLLSRYFVDQLAEQGGPLEVDYLDLAH
                     DCPPHVTGDFARATYTPAEARTAEMHALLAPSDRLCERLLAADALVFAMPMYNWSMPS
                     NFKAFIDAIVRTGITYVMTPDGRYEGQLGGRPVLFITTRGADLGPGSPFAAMDALTPA
                     LRAAFGFIGVADPLFVDAQPVQFADPEARLAALDRARAQLSAIAGQWARQPARQPA"
     gene            complement(31911..32450)
                     /locus_tag="VFF98_08110"
     CDS             complement(31911..32450)
                     /locus_tag="VFF98_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU63632.1"
                     /translation="MAAHISTGVEYALHSLLFLADRAEEAPAPSTRDLAELQAVPPEF
                     LAKLFTRLQKAGLTRSIEGVGGGVRLARPPAAIRVLDVVRAIDGDKPLFECREIRQRC
                     VLYGDQAPDWATRGPCAIHAVMIAAQARLDEALAACTLADLAGQLRAKAPPAFGAAAG
                     GWLDARAARRTRKRKEKNA"
     gene            complement(32518..>33221)
                     /locus_tag="VFF98_08115"
     CDS             complement(32518..>33221)
                     /locus_tag="VFF98_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63633.1"
                     /translation="LPVRRALLDLRSALEDEPFEPGYTRQRFAIAVNNYAAIALAGAI
                     VLRARALAPGVQIRLRPSGTLDVADLLDRGELDLALVSGAPPLARYGSALLIEDDFVA
                     VVRQGHPLLGAPLDSAGFAAQAHLTISSSGDDLTGLDEALARQGLARNSEIEVPYLSA
                     GAVLTQSDMMAIMARQIAVAFARVHPVALVELPLQPAPLRQMLVWQRRFEDQAAQRWL
                     RETIASVAADLRSAR"
BASE COUNT         5576 a        11728 c        11005 g         4912 t
ORIGIN      
        1 ggaaaacacc gccgccgccc tggcccctgc cgccgccgac cagccgtctg ccgcccacca
       61 ccgcgccacc gatgccgcaa gttcctgaaa aaggcccgag gatgaaccac aaccctgtct
      121 atcttgcgct tgatctgccc cgcctggatg cggccgaggc actggcgcgc cgcgtgaagg
      181 atcatgttgg cggcatcaag ctggggctgg aattcttctg cgcccatggc caccatggcg
      241 tgcatgatct ggcaagggtg ggcctgccga tcttcctcga cctgaagctg cacgatattc
      301 cgaacacggt tgccagcgcc atgcaggcga tcaacgtgct gcaaccggcg atcgtgacga
      361 tccatgccgc aggcggccgg gcgatgatgg aagacgccaa ggcagctgct ggcgaacaca
      421 ccaaggtggt cgcggtcacc gtgctcacca gcctcgatgg cggcgatctg gccagcatcg
      481 gcgttgccgc ccagcctcac gagcaggccc tgcgcctggc cgaactggcc catagcgccg
      541 ggctggatgg cattgtctgt tccggccacg aggtgggcgc ggtgcatcgc cagtgggccg
      601 atggcttctt cgtggtgccc ggcctgcgcc cggcagagca ccaccatgcc gccaatggcg
      661 cggcgggcga tcagaagcgc atcgtcaccc cgcgcgccgc gcgcgatgcg ggcgccagcg
      721 tgctggtgat cggccggccg atttcccgcg cggaagatcc gctggccgcc gcccgcgcga
      781 tcgaggcaac gctctaggcg ttcatcatcg gctcagcccc cgggccccat ggcggcacaa
      841 gcccgcctcg ggcctgttcg ggagcgacac cgatgccttt tgcccccctg cctgccactg
      901 ccgccatcgc tttcatcgcc gccgcgctga tcggcacccc cgcgcttgcc gccgccccgg
      961 cagagctgcc cgccggccac acgctgctga cgctgagcgc gcaggggcaa tccgcccgcc
     1021 agcccgacat tgccggcttt tccgcaggcg ttgccaccgc cggccgcaat gccgccgagg
     1081 cgatggccgc caatgccgcc gcgatgagcc ggctgatcgc cgcgctgaag gccgccggca
     1141 tcgagccgcg cgatatccag accagccgcc tcgccctcac ccccacctat gccgagcgcc
     1201 gccccggcga cacccgcgcg ccggccatca ccggctatca ggccgccaac accgtggccg
     1261 tgcgcgagcg ccatgtggcc gaggccggcc gggtgatcga cgcgctggtg gcggccggcg
     1321 cgaatgacat ctccggcccc gatttcaccc ttgccgatcc gaaggcagcc gaggacgaag
     1381 cccgcgccga tgccatcgcc cagttgcttg cccgtgcgcg gctttatgcc caggcggtgg
     1441 ggctgaaagt ggcgcaggtg cgcgcgatca gcgaattcgt gcccagccag cccatgccca
     1501 tggcccgcag catggcgctg atggccatgc cggccgccgc aacgccggtg gaagcgggcg
     1561 aggtggaaag ccagatccgc gtgacgatgg agttcgacct cggcccggcc tgactcgccc
     1621 ctgcttgact ggcccctgcc tgacgcgccc tcttgacggc ggcgccggcg cgagccacct
     1681 cgcaggccat gtctgacacc ggccccgcca tcaagatctg cggcatcacc accagcgcgg
     1741 cgctggccgc cgccatcact gcgcgcgcgc actatgccgg cttcgtgttc tttgccaaaa
     1801 gcccgcgtaa cctggcgctg gccgattgcg gccccttgcg cgatcaggcg gcggggcaga
     1861 tcggccgggt ggggcttttc gtcgatgccg gggatgacgc catggccgag gcgattgccg
     1921 ccgccggcct cgatgtgctg cagctgcatg gcagcgaaag ccccgagcgc gtggcgcagg
     1981 tgaaggcgcg ctttggcctg ccggtgtgga aggccctgcc ggtggccgcg cccgctgatg
     2041 tggcgcgggc cgatgcctat gcccgcatcg ctgatctcct cctgttcgac gccaagacgc
     2101 ccaagggcgc gctgcccggc ggcatggggc tcgccttcga ctggcagctg gtgaagggct
     2161 ttcgcgccgc gccctggggg ctcgcgggcg ggctctcgcc ggccaatgtg gcagaggcag
     2221 tggcggtatc gggcgcgccg ctggtggatt gctcctcggg cgtggaaagc gcgccggggg
     2281 tgaaggaccc ggcgctgatc gccgccttct gccgggccgc gcgcggctga ggctgccagc
     2341 ccaaaagcga aagggcggcc ctccgggaag tgtggagggc cgccctgctc ccgccgggaa
     2401 agatcaggcg gcgggaggga aggccgggat cagaacttga agcccacgcc gatcagcgcg
     2461 gcattggcat cggtgttgcc atcatcgagg aactggtggc gatattccag cttggcgaag
     2521 gccggaccga agctgtgctc gatgccggcg cccaccaccg aaccatcggc gcccagccca
     2581 tagaccgcgc cgcccagcgc atagaccttg ttgccattgc cgatcgccgt gcccagccgg
     2641 cccacggtgc tgaagcgcag cttggcgcca tcggcctgca cgcggtccat gtcctcttcc
     2701 aggcccatga aggcgttgga cgagatgttg aaatcatagc cgacgccgat gccgacatcc
     2761 gccttggccg actggccatc gttccagccc acacccgtat gcacatcgac atgggtttct
     2821 cctgccatcg caggcgttgc cgcaagggcg gccgcagctg cggaaataag gatcaggcgc
     2881 atatgtatcc tccatgaatt caatggtttg ttacccctgc cgccgatgtg cgccccggcg
     2941 ccttgccgat cgctgaatgg ccatttcagg aaactgtcag gcttgtatca gcgccggccc
     3001 cgcaaggccc cgccaggggg tgcggcccgc gcccgcccac acccacgcgc gcactggaca
     3061 gcagcgcgcc gctctgccaa tcgcatgggc catgacaaac cacaccgctc ctgccaattc
     3121 cctgcgcaac cagcccgacg aacgtggcca tttcggccag tttggcgggc gttacgtggc
     3181 cgaaacgctg atgccgctga ttctcgatct ggaacgggaa tataccgccg ccaaggccga
     3241 tcccgccttc catgccgact tcgaggacct gctgacccac tatgtcggcc ggcccagccc
     3301 gctctacttt gcgccccgcc tcaccgagga gttgggcggc gcccaggtgt ggttcaagcg
     3361 cgacgagctg aatcacaccg gcgcccacaa gatcaacaat tgcatcggcc agatcctgct
     3421 ggcgatccgc atgggcaaga ctcgcatcat cgccgagacg ggcgcgggcc agcatggcgt
     3481 ggccacggcc accgtgtgcg cgcgctttgg cctgccctgc gtggtctata tgggcgccac
     3541 cgatgtcgcc cgccaggcgc ccaacgtgtt ccgcatgaag ctgctgggcg ccgaggtggt
     3601 gcctgtcacc tcgggcgcgg gcacgctgaa ggatgcgatg aacgaggccc tgcgcgactg
     3661 ggtggcgaat gtgcacgaca cgttctacat catcggcacc gccgccggcc cgcaccccta
     3721 tcccgagctg gtgcgcgatt tccagagcgt gatcggcaag gaagcgcgcg cccagatgct
     3781 ggagcgcacc ggccgcctgc ccgatctgct ggtggccgcg atcggtggcg gatcgaacgc
     3841 gatcggcctg ttccacccct tcctcgatga tgccagcgtg aagatgctgg gcgtggaagc
     3901 ggccggctat ggcctagaca aggatcatgc ggcctcgctg gccggcggcc ggccgggcat
     3961 cctccacggc aacaagacct tcctgctgca ggatgaggac gggcagatcc tggaaggcca
     4021 ctcgatctcg gccgggctcg attatcccgg catcgggccc gagcatgcct ggctgaagga
     4081 agtgggccgg gtggattaca cctcggtgac cgacgaggag gcgctgggcg gcttccagct
     4141 tctgtgccgc accgaaggca tcatcccggc gctggagccg gcccacgcca ttgccgctgt
     4201 ggcccgcgtg gcgcccacca tgcccaggga cagcatcatt ctcgccaacc tgtgcggccg
     4261 gggcgacaag gatatcttca ccgtggccaa gcatctgggg atcgatctgt gagccgcctc
     4321 gcttctgcct tcgccaaggg ccgcccggcc ctcgtcacct tcgtcaccgg cggcgatccg
     4381 acgccgcagg caacccctgc catccttgat gcgctggtgg ccggcggcgc cgatgtgatc
     4441 gagctgggca tgcccttcac cgatccgatg gccgatggcc cggccattca gcaggccaac
     4501 ctgcgcagcc tgggcgccgg caccaaaacg gccgatatcc tgcgcatcgc cgccgatttc
     4561 cgcgcgcgcc atgccgatgt gccgctggtg ctgatgggct atgccaaccc gatgatcacc
     4621 cgcggcgccg aatggtttgc cagcgcctgc gcagaagcgg gcgtggatgg catcatctgc
     4681 gtcgacatcc cctcggaaga agacccggaa ctggggccgg cgctgcgcgc ggccggcatc
     4741 gccttcatcc gcctggccac ccccaccacc gacaccgccc gcctgcccgc ggtgctgcaa
     4801 ggcgctggcg gctttctcta ttacgtctcg gtggccggca tcaccggcaa gcagcaggcc
     4861 cagctggcta gcatcgagga ggccgtggcg cgcctgaagg cggcaacgga tctgcccatt
     4921 gccgttggct ttggtgtgcg cacgcccgaa caggccgccg ccattgccgg ccatggcgat
     4981 ggcgtcgtgg tcggctcggc gctggttgat ctggtggccg cccatggcgc cgatgccgcg
     5041 ccccatgtgc aagcgctggt ggccagcctt gcccaagctg tccattccgc acgaaagatc
     5101 tccgcatgag ctggctcgac aaggtccgca attcgctcac ctcgctggcc aaccgccgcg
     5161 ccgaggatgc aacgcccgac aatctgtgga ccaagtgccc ccattgcggc gccatgctgt
     5221 tcaccaagga atatgaggca aacctctccg tctgcccgca gtgcgaccat catggccgca
     5281 tcggcacgga tgcgcgcctc agccatctgc tcgatcccga ctatcagctg ctcacctcgc
     5341 ccagggtgaa ggaagacccg ctgaaattcc gcgattccaa gcgctatacc gatcggctga
     5401 aggccgcgcg cgccgccagc ccgcatatcg atgcgctgac caatgcgctg ggcgccatca
     5461 agggccacaa ggcggtggtg ggcgtgcagg atttccactt catggccggc tccatgggca
     5521 tggcggtggg cgcggccttc gtggcgggcg cccagcgcgc gctgacggag aaatgcgcct
     5581 atatcatctg caccgcggcc ggcggcgcgc gcatgcagga gggcatcctc tcgctgatgc
     5641 agatgcccaa gacgacggtg gccgtgcgcc ggctgaatgc ggccggcctg ccctatatcg
     5701 tcgtgctgac ggatcccacc accggcggcg ttaccgcctc ctatgccatg ctgggcgata
     5761 tccacattgc tgagccgggc tgtctgatcg gctttgccgg ccagcgggtg atccagaaca
     5821 ccatccgcga aaagctgccc gaaggcttcc agcgtgccga atatctgcac gagcacggca
     5881 tggtggacat ggtggtgaag cgccatgacc tgcgggatac gctggccagc ctgctgtcct
     5941 atctcagccc cgccaaggcg gcctgaagcg caaaggccag ctgcccatga aggattttgc
     6001 cacgcacgac caccccgccg tggccgccca gctggcgcgg ctgtcggcgc tgtcggtgcc
     6061 gcaggggcgg ctggggctgg aggtcatcac cggcctgctg gccgcgctcg gcaatcccga
     6121 gacgcggctg ccgcccacct tccacgtggc cggtaccaat ggcaagggca gcacctgcgc
     6181 ctatctcacc gccatgctgg aagcggccgg gcaccgggtg cattcggcca ccaagccgca
     6241 tctggtgcgc tataacgagc gcattcgcct agggggccgg ctgatcgggg atggccatct
     6301 ggccgccttg ctggccgaag tgctggatgc cgccgccacc ctgccggaag aactctcgcc
     6361 cagcttcttt gaagtgacga cggcggcgat cttcctcgcc ttctcgcgtg agccggccga
     6421 tgcctgcgtg atcgaggtgg ggctgggcgg ccggctggat gccaccaatg tcatccccgc
     6481 gccccttgcc tgcggcattg ccacgctggg gatcgaccat gaagccttcc tgctgcgccc
     6541 ggaagcgggc gcacccgaag gcccgctggc ccgaatcgcc tgggagaagg ccggcattgc
     6601 ccggcccggc cgcccgctcg tcacccaagc ctatcccgag gaagcggccg aggaagtgcg
     6661 ccgcgtggcc gcagcggccg gcgcgccgct gctgatgcgc ggcgaaagct gggaagcggc
     6721 ggtgggcgag gccatccatt accgcgacgc cgatggcctg ctgacgctgc cgctgcccac
     6781 actggccggg cgccaccagg ccgacaatgc ggcgctggcc atcgccatgc tgcgcgcgca
     6841 atcggtgctg acggtaagcg aggcggccat ggccgagggc atccgcgccg cgcgctggcc
     6901 ggcccgcctg caattgctgg gacaagggcc gctgaccgag ctggcccccg gccgcaagct
     6961 gtggctggat ggcgggcaca atccggatgc gggcgcggcc attgcccgcc atttcgccga
     7021 tcatccgcca ctgcatctca tcatcggcat gctggccaac aaggatccgg cagcgatcgt
     7081 gcggcctctg gcgggccagc tgctcagcct gtcggtagtg ccggcgccgg gccatgatgc
     7141 ccatgcgccg gaagccttcg ctggcctcac cgatctggcc attgccccgc gcgccgatgt
     7201 ggcagaggcg ctggccgcgc tggccacgcc gggcgatgtg ctgatctgcg gctcgcttta
     7261 tctggccggc gaggtgctgc ggctcaatca ggaatttccg gactgatcct ctgcatggaa
     7321 tggccgggcc cgcgcgccca gccattccaa cccgacaaag gcgacagaga ccagccctgc
     7381 cagcgccgtc cagcggaaca ccggggcata gccgtaatgg tcgaaagcct cgccggcgat
     7441 gccgcggccc agcgtgccca ccagcgacac cagcgaggac agcagcgcat actggatcgc
     7501 cgtgtagcgc ttgttcacca cgcctgacac ataggcgacg aatgccgtga gcgcgaggcc
     7561 ggtggaaatg ttctcgtagc agatcgccac cagcaggcgc agcagcgccg gccccgcccc
     7621 cagcgaggcg gccagcccat ccagccgcag cagatgggca aaggcatcga tcatccgccc
     7681 gccatcggcc agatcggcat agagcagatt gcccagcggc ggcagcaccg cgcccaccac
     7741 catggtggca aaccgccccg cccgcgcaaa cagcaggccg cccaggctga tgccgatcat
     7801 cgtcatgaaa atgccgaaga tcttggaggc aaaggcaacc tcctccttgg tgtagtgcag
     7861 cgcatccaga tagaagggat aggcaaagct ggcccagata ttgtagcaca gcgcatagga
     7921 gaggatgaag ccgatgaggc tgagcaccca gatcccccgc cgcgccacca aatcttccag
     7981 cggcgccacc agcgcgccat agagatgatg gcgcgccgcc ccccacaggc ccgccatgcg
     8041 cacctgccgc accgctcccc ggcggcgcag cgcatgggcc agcgccgcca ccgccagcgg
     8101 caccagcacc gttgccacca cgatccacgg gcccgtgccg cgcgtgaagg ccgccaccga
     8161 caccggcgcg ccatggtgat ggcgggccgg cgccagcatc tgcgccatga agcccaccag
     8221 gctggccacc gcccagcccc agctggccag caccagcgcc agcagcagcg cctgcccgcg
     8281 cggggcctgc tgcgtgccgg cctcatcggt ggcgggggtg cgggcggggg tgtcgggcgc
     8341 ggcaaggcaa gccagcgcca cggctcccag catggccgcc atggccagaa acacctgcgg
     8401 ccagcgcata tgggcggcca tcaacagcgc aaaggccccg cccacgatcg aggcggtgcg
     8461 atagccgaac tgatagatcg cgctcagcag ttcgacgctg gtggcggcat cggccacatc
     8521 gatgcgccag gcgttgatcg cggtgtcctg cgtggccgag gccagcgcgc acaggaacgc
     8581 caccgctgca aacggcccga tcgccgcctt gggatccagc tgcgccagca ccaccagccc
     8641 tgccaccagc accgcctggc aggggatgat ccagcttttg cggcggccca gccgggccag
     8701 cagcggcagc cgcagccgat ccaccaccgg cgcccagagg aaggcgaagg aatagcacag
     8761 gccgatccag ctgaagacgc cgatcgtcgc cagcttcacg cccacctcgc ccagccaggc
     8821 gttcagcgtg ccgatcagca gggcaaaggg caggccgctg gcaaagccat agcccagcat
     8881 cgccgccggc ttggcgcccg acagggccgt ggcgagaagg cgcaggcccc tggggggctg
     8941 ctgggaagac ggctcggcca tggcgggaat cggttatgcc cctgaaactg cttggcgcca
     9001 ccctaggggc gcgggcgccg cgcggccacc ccaaagcgcc gcttttccac cgccccccac
     9061 cgcccgcttt tgacgcggcg gcgcgaggaa ggtaaggcgg ggcatggcaa aacctccctc
     9121 tcgcacgcct tcctcgcgca gcacccgccc cggtggcgag cgccgcccct ctggcgaacg
     9181 ccgctctcct ggcgaacgcg gccctggcgg tgaacgccgc gcctctggcg agcgcggcgc
     9241 cgccaacgaa cgcggcgcct ctaacgaacg cagcatcgaa cgtggccggc ccattccgcc
     9301 gccgcgccag cgccgcccca cgcccgcctg ggccaagccc gcgccggcgc ccgccaaccc
     9361 catgccccgc ggcgagggcg aggagcgcat cgccaagctg ctggcccgcg ccggcatcgc
     9421 cagcaggcgt gaggtggaac ggatgatcgc cgagggccgg gtgaagcgcg gcgaggaagt
     9481 gatcgccacg ccggccacgc tgctcaggag cctgaagggc atcaccgttg atggccgggc
     9541 cgtttcggcg cccgagccaa cgcggctgtt cgccttccac aagccctcgg gcctgctgac
     9601 ggcagagcgc gatccggccg ggcggccgac catctacacc gccctgcgca acgccctgcc
     9661 cgccggcacc ccgcggctga tgccggtggg ccggctcgat ctcaacaccg aggggctgct
     9721 gctgctgacc aatgatggcg agctgaagcg cgcgctggaa ctgcccagca gcggggtcac
     9781 gcgcacctat cgcgcccgca ccttcggcga tatcacccag gaccggctgg aagagctgat
     9841 ggaagggatc gagatcgacg gcgtccgcta tggcagcatc gatgccaata tggagcggcg
     9901 caccggccgc aaccagtggg tggaactgac gctgaccgag ggcaagaacc gcgaagtgcg
     9961 ccgggtgctg gaacatctgg gcctgcaggt gtcgcgcctg ctgcgcgtgg cctatgggcc
    10021 gttccatctg ggcgatctgc cgcgcgggca ggccgtggcc atccgccaga tcgaggtgga
    10081 gcggctgcgc aagagcctgc gcagcggcaa gggcgccaac acgccggcgg ccatccccgc
    10141 cgaccagacc ccacaggcga aggacaactg atgcgcatcg tggccggaga atggcgcggg
    10201 cgcaagctgg ccgcgcccga tggcgatgcc acccgcccca ccgccgatcg cacgcgcgag
    10261 acgctgtttt ccatgctgct gtcgcggctg ggggattttg aagggctgcg ggtggccgat
    10321 ctgtttgccg gatcgggcgc gctggggctg gaagcgctga gcaggggcgc cgcccacgcc
    10381 ctgtttgccg agcaggatgc aagcgcgctg cgcgccattc gcgccaatat cgccaatctg
    10441 cgcgcgcagg cgcaatgcga tgtgcgggcc ggcagcgtgc tggcgctggg cccggtgaag
    10501 cagccctacg acctcctgtt cctcgatccg ccctatcaca gcggcgccgg cgccgtcgcg
    10561 ctcgacaagc tgaagcggct gggctggatt ggcccggcga gctggatcgc gctggaaacc
    10621 gaagcgcgcg aggaacccaa actgcgcggg ctggaagcgg tggcagaacg caccgtgggc
    10681 aaggcgcgca tcaccctgct gcgcctgccc gccagcgcgg aaagccgcct aggctaggcg
    10741 gccgcaggca ccggggccgg ccgcgccagg ctgaccccgg ccagcgccag caacccggcc
    10801 accgccagaa tgagccccac cgccgcgccc cagccaccgc gcgccagcac ttcggccgcc
    10861 agcgggctga gcgcgccgcc cagaatgccg cccaggttga aggccagcga aatccccgtg
    10921 tagcgcagcg gcaccgggaa cagcgtgggc agccagcccc ccagcgggcc atagaccagc
    10981 cccatcacca gcagcgccgc gctgagcgtg acaaacaccc acaccagcga gcccgacagc
    11041 agccccggcc caaacgccag ccccaccagc acggtgagca cgctgcccca gcccagcacc
    11101 tcgccaggcc gggtcttgtc ggcccagatg gccgccagca cgatgcccac cgcaaagaac
    11161 aggttggcgc ccagctgcac cagcaggaaa tcggtgcggt tatagcccag cgccccggtg
    11221 cccagcgaca gggcaaaggc agtggccaga tagaacagcg caaaacacgc caccaccccg
    11281 gcagagccgg ccatcaacac cccgaaatgc cgccgcagca gataggccag cggcacgcgc
    11341 acccgggtct tgcgggcaat ggcatcggca aaggcggggg tttcggcaat gcgcagccgc
    11401 acccacaggc ccagccccac cagcaccgcc gacaggagga acggcacccg ccagccccaa
    11461 gcaagaaact ctgccggagt gaggcagacg cccaggatca ggaacagccc gttggccgcc
    11521 agaaagccga tcggcgcgcc cagctggggc gccgcgccaa agcgggcatg ccagccttcc
    11581 ggcgcatatt ccaccgccag cagcgccgcc ccgccccatt cgccgcccag gccaaagccc
    11641 tggccaaagc gcaaggtgca cagcagcgcc ggcgccagcc agccagcgct ggcataggcc
    11701 ggcagcaggg cgatggcgaa agtgcagccg cccatcagca acaggctggc caccagcgtg
    11761 ctcttgcggc ccaccctgtc gccaaaatgg ccgaacagca ccgcccctat cggccgcgcc
    11821 acaaaggcaa tgccaaaggc catgaagctg tagatcccct gcgcggccgg cgaggcggcg
    11881 ggaaagaaca gcgggccaaa caccagcgcc gttgccgtgg cgaacacgta gaaatcgtag
    11941 aactccaccg tcgtgcccac aagggcggcg gcgaggatgc gccggtgctg gcgcagggga
    12001 gacaggagcg aggtgggcgg ctgattcatc cctatgggct gccgccttgc ggccggtgcg
    12061 tcaaacattt tccttgtggg cactgcaaca taggtggccc gcagcggcac accctgtgca
    12121 aaacaccagc tttttgttgg ggccattttg gcggcgcccc ttgcaagcgg cggatttgtt
    12181 ccctacccgt tccagtgtga ccacagcccc cccttcgatt gccgccatgg acgataccac
    12241 ccttcccgcc aatgcccccg aatggctctc ccggctgaac acgccccagc gcgaggcggt
    12301 gctggccagc gagggcccgg tgctgatgct ggccggcgcc ggcaccggca aaaccgcggc
    12361 gctgacggca aggctcgcct ggctgatcgc ctgccgcaag gcctggccat cagagatcct
    12421 gtgcgtcacc ttcaccaaca aggccgcgcg cgaaatgcgc gaacgcgtgg gccatctcat
    12481 cggcgatgcg gtggaaggga tgccctggct gggcaccttc cacgcgattg ccgccaagat
    12541 gctgcgccgc catgccgagc tggtgggcct gcaggccaat ttcaccatcc ttgacacaga
    12601 cgaccagctg cgcctgctga agcagctgat ccaggccgag gggctggacg aaaagcgctg
    12661 gcccgcccgc cagctggcca gcctgatcga ccggtggaag aacaaggggc tgggcccaga
    12721 cgagctcgac gcgctggaga acgaggccta tgccaatggc aagggcgcgc atttctaccg
    12781 gctctaccag gcccggctga tcacgctgaa cgcctgcgat ttcggcgatc tcttgctgca
    12841 catgctggcg atcctgaagc gccaccgcga tgtgctggag atgtatcagg cgcgcttcaa
    12901 atacatcatg gtggacgaat atcaggacac caatcaggtg cagtatctgt ggctgcggct
    12961 gctggcgcag ggcgtggaag ggcgccgcca caacatctgc gtggtgggcg atgacgacca
    13021 gtcgatctac agctggcgcg gcgcggaagt ggccaatatc ctgaagttcg agaaggattt
    13081 tcccggcgcc cgggtgatcc ggctggaaca gaactatcgc tccaccccgc atatcctggg
    13141 ggcggcggcc ggcctcatcc gcgagaatgc cgagcggctg ggcaaggagc tgtggaccga
    13201 ggcgaccggc ggcgacaagc tgcaggtgat cggcgtgtgg gacgcgcccg aggaagcgcg
    13261 ccgcgtgggc gaggagatcg agcggctgga gcgcgagggg ctccccctca cccatatcgc
    13321 catcctcgtg cgcgcccagt tccagacccg cgaattcgag gaccgcttca tctcgattgg
    13381 cctggcctat cgcatcctcg gcggcttccg cttctacgaa cgcgctgaaa tccgcgacgc
    13441 gttggcctat ctgcggctgg tggcgcagcc atcggacgat ctggccttcg agcggatctg
    13501 gaacacgccc aagcgcggcc ttggcgacaa gagcatggaa aagctgcacc gctttgcccg
    13561 cgctgccaac ctgccgctgc tggcagccgc gattgccatt gccgataccg acgagctgcc
    13621 cgcgcgcgcg cgcggcacgc tgctggcgct ggcccgcgat tttgcccgct ggcgcgatct
    13681 ggccgccagc gagagcccgg ccaacctggc ccgcacgatc ctcgacgaat cgggctatac
    13741 ggcggcgctg caggtggaaa aatcggccga aagcgccggc cggctggaaa acctcgtgga
    13801 actggcccgc gccatggagg aatacgagag cctcgccgac ttcctcgaac atgtcagcct
    13861 cgtcatggac aatgacgcga atgacgatgc cgagaaggtg acgatcatga ccatccacgg
    13921 cgccaagggg ctggagttcg accatgtgtt cctgcccggc tgggaggaag gcgtgttccc
    13981 ctcgcagcgc tcgctggacg agggcgggct ggccagcctg gaggaagagc gccggctggc
    14041 ctatgtggcg atcacccgcg cgcggcgcca ctgcacgatc ctgcatgccg ccaaccggcg
    14101 catctatggc cagtggacct catcgctgcc cagccgcttc atcggcgagc tgcccaaggc
    14161 gcatatcatg gaggaaagct cgctgtcggg cggggcctcg ctgtggcgcg cgcagtggag
    14221 cgagcgggcc gatcccttcg cccatgtggc gcaggaacgc agcgcccggg ccgaaacccg
    14281 cggccccggc tggcagcgcg ccagccagca gcccttcacc cccacgcccc gccgcatcgc
    14341 cgagcccacg cgcagcgccg ccagctttgc cgccaaggcc cggctcgaca tccgcccggg
    14401 cgaccgcgtg gtgcacgaca aattcggcac cggcaccgtg gccgcgcagg aaggcaacaa
    14461 gctggagatc gatttcgaca gcgccggccg caagcgggtg atcgacagct tcgtgcggcc
    14521 agccagctag gcacagcaaa acgggcgagt gggacagccc cctcgcccgt tgaaaccctc
    14581 gcgtgctgaa acctcagtgc agggctgaaa tgccgaggaa ttcggggatc ggcccgttcc
    14641 attcgccggg cctggtcggg ccgtcatcgt cgcgccagcg cgggcggcgg tcctcgcggc
    14701 ggtctgcccg ccgctcgtca ctgcgcgcat cacggcgctc gtcgcgctcg ggccgggcgg
    14761 cccgttccgg cctggccgca cgcgccggct tttcggcccg ggcgggcttt tcggcgcgca
    14821 ccggcttttc ggcccgggca ggcttttcgg cacgagcggg cttttcggct tccacctccg
    14881 gctcgggcgc ggcctgccgg tcagggcggc gccggtcatg cggggcagcc ttgcggccgc
    14941 gcgcggccga acggggcgcc ggcgcgtcat cgccctcgtc cacaccgccc agctcgacga
    15001 ccatttccgg aatgggcccg cccgtcagct tctcgacact ggcgatcgcc tcggcatcct
    15061 cgctggtgaa cagcgtgaag gcgcggcctg tggccccgcc gcggccggtg cggccaatgc
    15121 ggtgcacgta atcgtccggg tgccagggcg tgtcgaaatt gaagacgtgg gaaacgccct
    15181 tcacatcgag cccgcgcgcc gccacatcag aggcgcacag gatattcacc tgcccgtcct
    15241 tgaagcgctg cagttcggcc aggcgcgccg gctggtccat gtcgccatgg atttcgcctg
    15301 cggcaaagcc gtggcgcttc aggctcttgg ccagatcgcg cacggtggtc ttcttgttgg
    15361 cgaagacgat cgccgtcgtc acattatcca cctgcagcag gtggcgcagc atttcgcgct
    15421 tgcgcttggc ggccaccttc accttgtgct gggtgatgtt ggtgttggtg gtggcagggc
    15481 gcgccacctc gatatatttg gggttggtca ggaagcggtc ggccagcttc ttgatcggcg
    15541 gcggcatggt ggcgctgaac agcagcgttt gccgctgggc gggcagcttg gtgcagatgt
    15601 tctcgatatc ggggatgaag cccatgtcga gcatgcgatc cgcctcgtcg atcaccagca
    15661 ggttgcagcc ggtgagcagg atcttgccgc gctcgaacag gtccatcagc cggcccggcg
    15721 tggcgatcag cacgtccacc ccgtccgaca gcgccttcac ctgatcgccc atctgcacac
    15781 cgccaatcag cagcgccatc ttgagatcgt ggttcttgcc gtatttctcg aaattctcgg
    15841 ccacctgcgc ggcgagttcc cgggtcggct cgaggatcag gctgcgcggc atcagcgccc
    15901 ggcggcggcc atgggccagg atgtcgatca tcggcagcac gaagctggcg gtcttgccgg
    15961 tgccagtctg cgcgatgccg ataaggtcgc gcatcatcag cacggcgggg atggcctgcg
    16021 cctggatggg cgtgggcgtg tcatagccgg cggccgcaac gctctgcagc aattcttccg
    16081 acaggccgag atcggcgaaa ctcatgagct tttcgtgaaa tccgttctgg gggccagcgc
    16141 caaaccgttt gccgcccttg ccccacgatc cgcccatggg ccgcatggcc ccgggcaaac
    16201 cccgagcgcg cttatcgccg agccggagaa aaagtcaagg aaatcgcctc ccgggcacgg
    16261 taatcagcat gcgcagggct ttcaaacggc aattcatgcc cgtgcgcgac tgcaatttgt
    16321 cgcgcccggt gcagatcatc ccgtcggggc tgcgctgcat atagaagccg gaatagaagt
    16381 catgcgcgcg gcaggtgcgc tccagcacgg ccgagatgat ccgctggtcg cgcatgaaca
    16441 gcagcaggcg gttgccctcg ccgctttgca cgcccagaat gttgctcatc gccacgcagc
    16501 ggccgatgtc gcgctcgggc gtgggggcgg ggcgttcctc ctgctgggct tcctccagca
    16561 cgaaaggcgg catggccggc gagcccggcg tgatgcggat ggaaaaatgc tgctccaccc
    16621 gcacctgggc ggcggtctcg tcgggcaggc cgggcggttc gggcagatct gcgcgatcgg
    16681 gctgatcggg gatggcggcg cgcaaggccc gcaggccgcc ggtcaacggt tccggcagcg
    16741 ccatggcgag gcgcagcccc tctgcacgca aggaggccgg cagcccgcct gacagcacca
    16801 gggcggcgcc ggccaacatc cgggcggcac aggaaaaggg aaggcgaagc gggcggatca
    16861 tcagttctgg cggatcatct ggtcttgtgt tggccctgtt cctccctgct ggcgggcgcc
    16921 gcgcgcaaac cggcagggaa gcctgttcta gccgccgaga ttgaacctcg cgttaactgc
    16981 gcagggcccg cgtttcatgc cgatcgcagc gtggcgcgcc ggcaacaggc agcaccacat
    17041 ctggccagcg ggggcacggc tgtgccatag gcgggcgatg accgacaccg cctttcttgc
    17101 cgctgccgct gccctccttg gcccgcgcgg ccttgccacc gatgccgata ccctggcccc
    17161 ctggctgacc gactggcgcg ggcgctacac aggcgccgcc ctggcgctgg ccagccccgc
    17221 caccaccgcg caagtggccg cgctggtgca gctgtgcgcc gcccatggcg tggccatcgt
    17281 gccgcagggg ggcaattcgg gcatgtctgg cggggcaacg ccggatgcct cgggccgggc
    17341 actgctgctc tcgctgcggc ggttgaacgg gatcggcccg gtggacaggg ccgcgcggcg
    17401 cgtcacctgc ggggcgggcg tgatcctgca gcacctgcat gaggcggcag aagcggcggg
    17461 tcttcgcttt cccctctcgc tggggggcaa ggggtcggcc acggtgggcg ggctggtcag
    17521 caccaatgcc ggcggcacgc aggtgctgcg ccacggcaac atgagcgcgc aggtgctggg
    17581 gctggaagcg gtgctggccg atggcactgt gtggtctgcc ctgacgccgc tgaagaagga
    17641 caatcgcggg ccagacctca agcagctgtt catcggcgcc gagggcacgc tggggatcgt
    17701 caccgcggcc acgctgaagc tggcgccggc gattgccgac cggcgggtgg tgtggtgcgg
    17761 cgttgccagc ctgcagcagg cccgcgcgct gatgctgcat gccgaggcgg cgctgggcga
    17821 ggcgctggaa ggcttcgagg tgctgcccca ttccagcctc gaggcggtgg aggcctatct
    17881 gcccgatgtg cgccgcccgc tggccgcccc ccgcccctgg tatgcgctga tcgaggtggt
    17941 ggccgatacc gcctctgccg ccagcctggg cgagcgggtg gaagcggtgc tggccagcgc
    18001 cttcgaggcc gggctgctgg acgatgcggt gatcgccgcc aacgagaccc aggccgaagc
    18061 cttctggacc atccgcgaat cgatctcgcc ggcagagcgc gccaagggcc cggccatgca
    18121 gcacgatatc tcggtgccgg tggaagccat gcccgatttc gtcgatgccg cgatcagcgc
    18181 catgaaggcc cgctttgccg gcaccgaggc gatcgccttt ggccatctgg gcgatggcaa
    18241 tgtgcatttc cacgtgatcg cccccagggg cgtggaacgc gccgcctggg aagcgggcga
    18301 aggcaaggca atttcggccg cggtgcatga tctggtaacg caatggggcg gatcgatcag
    18361 cgcggaacat ggcattggcc agctcaaagt ggcggaactg ggccggctgg ccgatccggc
    18421 ccggctgaaa ctgctgcgcg cgctgaaggc ggcgctggat ccggccgggc tgctgaatcc
    18481 cggcaagctg gtgccacctg tctgaaacac ggggctggca ggggcggcag gctggccggg
    18541 aggcattttg ccttggcgtt ctaagcgttg ccccgcttgc gcgccggccg cccagcgcct
    18601 aaacccgccc gcgtttttcc gaaagtgcgg aacgaacccc aagggagtgc attgattatg
    18661 gccagcgcgc cacagaacgc cagcctgccc gttttctaca aggatctagt gccgctcaac
    18721 agccgcgagc atgccagctg gaaaagccgc accaccgatc gcgccacctg gctggtgggc
    18781 cagcatgccg tgccgctgac ggtggaggaa ttcccccagg cgcagcgcca cttcccgatc
    18841 gtcttctcgg tgggcgacaa ccccgtgccg ctggcgctga tgggcctgaa tgaaggcatc
    18901 aacgtcttcg tcgacgacga gggcaaggtg gccgacaaca tctacctgcc ggcctatgcg
    18961 cgccgctatc ccttcatgct ggcccgcctg acgcccgacg cgcaggaact gtcgctctgc
    19021 ttcgacccga acagcgacct gatcggcgaa ggcgaagagg gcacccccct gttcgacggc
    19081 gaggagccca gccagaccac caaggaaatg ctcggctttt gcgagcagtt cgagcaggcc
    19141 ggcgcccgca ccgccgcttt cgtgcaggag ctgatcgagc acaagctgct gatcgacggc
    19201 gaagtggcca tccgccagga aggcaacgag cagcccttca tctatcgcgg cttcctgatg
    19261 gtggaccaga acaagctgcg cgaagtgcgc ggcgatgtgc tgcgcaagtg gaaccagagc
    19321 ggcctgctgc cgctggtgat cgcccatctg ttcagccttg atctgatgcg cgaaatcttc
    19381 gcgcgccagg cgcagctggg caaggggccg ctggtcgccg cctgaggcgg gccgggccat
    19441 taggctgaaa agccggggtg ggccttgcgg cctgccccgg ctttttcatg cccgcaaggc
    19501 cgggcgattc ttgcaccagc gcgcacagca ggcgcagcac ctccctgccc cgggtcagcg
    19561 ctgttttgcc gggttcccgc cgggaatgcg cagaattttc tatccagcgg atagactttc
    19621 tttgcccatt tgcgcccctc tccttcggaa taggcttgaa agccgggcaa aggcagcata
    19681 ttaccgaatt gtcccaccgg cgcttccctc atggcccggc gggattggtg caccttggtg
    19741 cacctcctcc ctgaaccttg gccacctcgt gcggatgcac gaggtggttc ttttatggga
    19801 aaggcgcgcg gccgctctat tcagccgctt gcgcgcaaga tccgccagcc agctgagcgc
    19861 tgcccatcgc cgccgtcgcc tcgcccagcg cccgcaccag cgcgcacagc atcgccacgc
    19921 aagcggcaaa ccccggcccc ggcgccacca gctgcgcatc gagataggtc gtgcggcaga
    19981 tctccagctg caccgcatgg cgcccgcgcg ccggcatgcc atgccgatcc agcgtatagc
    20041 caccggcata gggccggtta tgcgcggcca gccagccagc cccggccagc gctgaaaacg
    20101 cctgcgccac cagcatgccg ccacagctgg cgccaaaccg atcgcccacc acgacctgcg
    20161 tgcggggcgc ccccggggcc gccggcaagg gcggcatcga atgcaggtcc accagcaagg
    20221 ccgcccccca gcgcgcctgc aaggcggcca gttccgccgc cacgcaggcg tgccagggcg
    20281 catgcacgct ggcaatgcgc gcctccacct cggccaccgg cggccggccc tgccacaatt
    20341 cgcccagccc cggcaggcgc cttggcacca gccccagccc gccggccgcc cgcccgcccg
    20401 cagggggcgc tagccggctt tgcggcgcgg cgccggcaaa catcgtccag tccacatcgg
    20461 ccgggtggcg gttgaggtcg atcatcgccc gcggcgcctc ggccaccagc agcgccgcgc
    20521 cggtggcttc ggccagagcc cggcccagct cgtcggccaa ccggtcttcc agcttcagcg
    20581 cagcctgttc gggatggcgc aggcgggcca gcagttccgg cggatagcgc cgcccggcat
    20641 gcggcacggc caccagcacc ggcagccgcg ccccatccgc gccgcacggc acgacaaagg
    20701 ccggcgcccc cgcaaggccc gggatcgccc cgcccgagag gggcgaatca gcctgggcgg
    20761 cgcgatcggt ttgggggcca tctgtcatcg cgcaaggctg gcccagcgcg cccttcctgt
    20821 caaagccgca ggccatgccg cgcccggccc ccgcgcgccg tgccaaatag gggcagggca
    20881 aaaatcgggc gagaatatta accccggcag gcgtatggag tggctcccga aaggccagaa
    20941 aaaagctatc gcacaaccat gacccgcatt ctccttgccg aagacgaaac cgccatgcgc
    21001 acctaccttg cccgcgccct cgaaaaggcc ggctatgacg tggttgcggt ggaccggggc
    21061 acggccgccc tgcccctgct ggaaagccag catttcgacc tgctgctctc cgatatcgtg
    21121 atgcccgaga tggatggcat cgagctggcc cagcgctgcg gcgagatctc gccggccacc
    21181 aaggtgatct tcatcaccgg ctttgccgcc gtgaccctga aggccaaccg cgaagccccg
    21241 caggcacgcg tgctctccaa gccctttcac ctcaaggatc tggtgctgga agtgcagcgc
    21301 gtgttcggcc attccagcga agtgtcggtg tgaacggggc ttgccgcatt tttctgcaaa
    21361 tccatgccaa aggcgcttgc atcaccggca aggcctcgct aaagcgccct cctcgccggc
    21421 gggaacacca cacccgccag cgctgatcga agtgagtggg cgtatagctc agtggtagag
    21481 cactgtgttg acatcgcagg ggtcgcaagt tcaatccttg ctacgcccac cattcgaaaa
    21541 acccgccagg ccaaaagccc aggcgggttt tttcatggcg cctgatcgcc ctggcgggcc
    21601 acgggcagat gccagccccg gcggatcgcc gccatccgca agccaaagca cagcacgccc
    21661 cccccagcgc cgagggaatg accggccagt gcagcgcgct gccgatcacc accaccgccg
    21721 cgccggccag cgccgccacc gcatacaggt cagaacgcag cacccgcggg atatcggaaa
    21781 ggaacagatc gcgcgccacc ccgccgccga tgcccgtcac catgcccagc agcgctgcca
    21841 tcaggggcgg caagccaaaa gccagcgcct tctccgtgcc cgacacggca aacagggcaa
    21901 gccccgccgc atccagcacc agcacaggat tgcgccattt ctccatcccg gcaaaccaga
    21961 agaagcagga cagccccgcc actaccgata cggccagata gcgccaatcc tgcagcgatg
    22021 ccggcgggat cgcgccgatc agcacatcac gggtgatccc cccgaaggtg gaagcggcaa
    22081 aggccagcac cagcaccccg aaaatatcga gccgccgccg cacgccggcc atcgccccgc
    22141 tgagcgcaaa ggcgaatgtg ccgcccagat ccagcaccag caccaggttc tgcagcagat
    22201 ccgctgtcgc ccgccctccg aacacggccg cgacatggcc aaaatcggcc atcaggcggc
    22261 attccatgcc agggcggcgg cggccatggc ttccaccccg atggccagcg tggggtgcag
    22321 cacaggcgcg aaatgcgggc tgtggttgac gggaagatcg ttcagccgcc cggccgcctt
    22381 ggcctgcgca taggcggccg gatcggttcc gcccacaaac cagaacaccg agggcgcctg
    22441 ccattccgtg ccgaagcagc cgaaatcctc gctcgcgggg gcgggccctg tgtgaaacac
    22501 ccgctctgtg gagaaatggg cgcggaaggc ctgggccacc cggtcgctgg cctgcctgtc
    22561 gttcacgttc agcggatagc tgtcgagcgg ggtgatctcg ggcggccggg gcgcacccga
    22621 tgccgcggcc tcggcctcca cgatccgccg gatcgcggcc agcacgcggc tgcgtacgcc
    22681 cgcatcgaag ctgcgcacgt tcagcttgat cagcgcctcg tcggggatga cattctcctt
    22741 cgtgccggcc tgcaacacgc ccaccgtgac caccgccgcc tcggtggccg ccacctcgcg
    22801 cgagacaatg ccctgcagcc gcatgaccac cgaggcggcc atgacaaccg gatcgacact
    22861 ggcctgcggc atagatccat gcgcgccgcg cccgaacagg cggatctcga aactgtctgc
    22921 cgccgaggtg atcggcccgg cgctgccggc cacggtgccc gccgccccca ccatgacatg
    22981 ctggcccagc acgatatcgg gctttggaaa ggcctcgaac aggccatcat cgatcatcgc
    23041 ccgggcgccc tgcgcggtct cctcgcccgg ctggaacaga acgatcagcg tgcccttcca
    23101 ggcatcgcgc gtgcgggcga acagggcgct ggcgcccatc agccaggcaa catgcatgtc
    23161 gtggccgcag gcatgggcca ccggcaccgc cttgcccgcc ggatcggtgg cggtggcggt
    23221 gctggcgtag gcaaggccgg tgtcctccag gatcggcagc gcatccatgt cggcgcgcag
    23281 catgacagtg ggcccctcgc cattgcgcat caggccgaca acgcccgtct ggccgatgcc
    23341 ggtggtcacc tcatacccct catcggccaa atgcctggcc gcaagggcgg ccgtgcgctg
    23401 ctcctgcatc gacagttccg ggtgggcgtg gatgtccttg taggccgcct ccagcgccgg
    23461 caggagatca ggcagcggcg ccagaagccg cttcagcgcc agggtctggt cggtgctcac
    23521 gcaaactctc cttcaggggc cacctgcgac agatttggga atacaacccc gatcgcccgg
    23581 catcaacggt ggctgttgcg taggccccgg gctcttctcg ccgcgccttc atcaggcggc
    23641 ggcctggctg gccatcggcg tggatcgcgg ctagcagcgg cgcgtggaag acctttttgc
    23701 cgaccggacg attcgcgata gccgcctgcg ccgccccgaa ctggcggcgc tgggcgcccc
    23761 ccaccggctg atcggctgcg atctggaaga agcggacctg tcggggctgg acctttccgg
    23821 ctgggtgttc gagcagtgcg acctgcggcg cgcccgcctt gccggcgccc gctgcgagcg
    23881 cagcctctgg caatcctgcc gcggcgcgtt tgccgatttc accggctgtg atctgtccga
    23941 ggcgcgcttt gtcgcctgcg atttcaacaa tgccatgctg cgccgcagca cgctggagga
    24001 ggcccgcttc ctgcgctgca aatgcaccgg cgccgatttc tccgaggtga aggcgatcac
    24061 cctccatatc gaggaaacgc tgctgatcga tgcccggctg gcgggccttt ccttccgcaa
    24121 ggcgcagctg gtgcgggtcg atttttcccg cagcgatctg cgcaaatgcg atttccgcat
    24181 gacctccttc gaggcctgca gcctgcgcga ggcgcaaacc cacggcgccc gcttcgagca
    24241 ggcagacctg cgcggggccg atctgggcgg catcggcctt ggcgatgccg cccgctttcg
    24301 cggcgccacc atctcgcgcg agcaggccgc ccagttgctt ggcgaacttg gcctcaaggt
    24361 gcgctgaagg cccgccgggc ttggccccat tatcgccgca gcgcaatcgc ccttccggca
    24421 tatggcgcgc catcgcccgc caccgccacc agggcgcgca ccgccgccag cagcccctcg
    24481 cccatggggg cagagcggcc gctggccgta tcgatatgga tcagcaattg ctcgcccacg
    24541 gccgccggcc catcgcggcc ggccacatgc agggcatgca gaaggtggat cttcttggca
    24601 tcatgggcca gcagcgtggt gcacacccgc accgcagcat cgcccttcac ttcggccaga
    24661 tagagaagat gcgcctccag cgtgtagagc gtggccgcct gggccttgcg ccctgccgca
    24721 tccaggccga tcagctccat cagccgatcg gtggcatagc tgaagatcag ggcataatag
    24781 gcatcgcgca gatggccgtt ataatcgatc cagcccggct caagcggccc ctcataggtg
    24841 atggtcatcg ccctttcacc gccgaggcaa ggcagcggtg gcgcgcagca tcgcggtaat
    24901 cagcgcatcg cgccgggcgg ccaattcggc ctggctggcg ctggcggctt cctcgttcac
    24961 gcccgccacc agcgcggcgg cggtcgcctc gtccagccgc ggatcgccca gatcctccca
    25021 ccagcggtgg aaactgtcag cgtatttctc gcagaaggcc gccagcccgc ccggtccgcc
    25081 gcccaggttg aacagcatcg ttggccccat ggccgcccag cgcagccccg gccccgccca
    25141 caccgccgta tcgacatcgg ccacgctggc agcgccgatc gccaccagat ggatcgcctc
    25201 gcgccacagc gccgcctgca gccggttggc gacatggccc ggcacctcgc ggttgacgcg
    25261 gatggtgacc ttgccaacgg ccgcatagaa ccgctcggcc agctctatca cgccgggcgc
    25321 ggtctgcgca ttgcccatca cttccaccag cgggatcaga tgcggcggat tgaacggatg
    25381 gcccaggata agccggcccg gatcgcccca gccggcctgc atctcgccca gcttcagccc
    25441 cgatgccgag gaagcaacca ccgcccccgg cttcagcgcc ggttcgattt cggcataaag
    25501 cgcatgtttg atggccagac gctcgggcac gctttcctgc acgaaatcgg catcgcgcac
    25561 ggcatcggcc gccgttgcat gaaagctgag cgcgccgctg cgccccgctt gcgccaggcc
    25621 cagttccagc agggtgggcc aggcatcgcg gatataggcg cgcaccatct gctcggcatc
    25681 aggcgccgga tcgaacaccg ccacatcgca gcccgctgcc agaaacagcg cgctccagct
    25741 ggcgccgata acgccggcgc ccagcacggc cacatgggaa atctcgctca tcagaataac
    25801 ctcggttgaa tgggacaggc agcggtcaac ccgccatcga cgacaaggca ctggccggtg
    25861 acaaagccgg cttcggcaga ggccagccag gccaccatat tggccacatc tgcggcgcgg
    25921 ccgaggcggc ctgcggggtg gcgcgccagc atatcggcgc gggcgcgggc cggatcagcg
    25981 gccgtggcaa agggggcatc ggccatggcc gtggcgatcc agcccgggca caccgcattg
    26041 cagcgcacct gcggcccgtg atcgaccgcg atcgcgcggg tgagcccatg gaggaaggcc
    26101 ttggaggcat tgtagagcgc catcgatgga tcggccgcaa ggcccgaaat cgagccgatg
    26161 ttgatgatgg ccccgcccgc ctgtgccatc agcggcagca ccgcgcggca catgttgaaa
    26221 gggccgcggg cattggcgcc catcagcagg tcccagtcat cgtcgctcgt gtcggccagg
    26281 gtcttctcga tctggatgcc ggcgttgttg acgagcaccg tgggcgggcc gaacaggtgg
    26341 caggcctcgg ccaccagcct ttcggcatcg gccgcctgcg ccacatcggc gcgcagccag
    26401 gccaccgctg cgggcaggtc cgccggccgc tcggcccgcc cgcaggtcag cacatgatcg
    26461 ccctgcgccc aaaaacgctc gacaatcgcg cggccaatcc cccggctgcc gccggtgacg
    26521 atgacgacct gcggctcagc catgggcgat cattacgtca tgggcgatca tcacatggcg
    26581 caccgccgta aaggcatcga gcgcgaagac cgacatgtcg cagccgtggc cagagccctt
    26641 catcgccgcc cagggcattt ccggcgtggc cacgccatgg gtgttgaccc aggtgaagcc
    26701 atagcgcagc cgggcagtgg ccgccatcgc ccggccggcg tctgccgtcc agacggagga
    26761 ggccaagcca tagcgcccgg cattggcgat cgccaccgga tcctcgtcgg ggccaaagcg
    26821 cgagacggtg acgaccgggc cgaagacctc gctggtggcg atctcggcgt ggttatcgac
    26881 atcggcgatg agcgtggggg catagaagaa cccaggccct gtaaccgccg ccccgcccac
    26941 gaccacctcg gcatggccgc gcgcccgatc cacaaagccg gccacccggt cacgctgggc
    27001 ggcgctgatc accgggccca tctgcgtgcc ggccgcgcgc ggcggcccca cgcggatctg
    27061 cgccacagcc tcggccagcc ccgccaccac gcgatcatac accgcatcgg ccacgatcag
    27121 gcggcagggc tgggcgcaat cctgcccggc gttgaaatag gcgccatcgc ggatggtggc
    27181 gacgagcctg tcgatatcgg cgtcggcgta aacgatcacc ggcgccttgc cgcccagttc
    27241 cagatgcacc tggcgcagct gcgcgcttgc cgcccgcagc gccgcccggc cggtggcggg
    27301 cgatccggtg atgctcaccg cctcgattcc ccgcgcgttg atcagctgat cgcccacgcc
    27361 cgcgccgctg ccgtgaagga tgttcaccac gccggccggc accacatcgg ccagcaagcg
    27421 cgccagccgc agggtcgaga gcggggtcag ctcggagggt ttgagcacca ccgggcagcc
    27481 ggccgccagc ggcgcggcca gcttccaggc ggccatcatc agcggatagt tccacggcgc
    27541 aatggccgcc acagggccga tcggatcgcg gcggatcagg ctggtgtggc ctgccacata
    27601 ttcccccgcg gccacccccg ccatggtgcg cgcagcaccc gcgaaaaagc ggaacgtgtc
    27661 gatggtgagc ggcatctcgt catcgcgggc agaaggccag ggcttgccga cattggcgct
    27721 ttccagcgcg gccagctccc cggcatgggc ctcgatctgg ccggcgatat cgagcagcag
    27781 cgcggcgcgc tcggcgggcg tgctctgccc gaaggtggca aaggcggcgt cagcggcggc
    27841 cacggcggcc tccacctggg cggcgctggc ctcgcccagg tgggccagcg tttcgccggt
    27901 ggccgggtcc agcacggtgg tggatgcgcc ctcgccggcc accagttcgc cgccgatcaa
    27961 cagttgcgtg tgcatcgcct gctcctcccc catgctcaga acgagacctt gtcgccgccc
    28021 ttcaggccaa ggcgctggcg ggcctcggcg ggcgtggcga tggtgagccc gaggttctcg
    28081 acgatcgagc ggatcttgcg gacctgatcg gcgttgcttt tggccttttc gccggggccg
    28141 atatagaggc tgtcttccag ccccacgcgc aaattgccgc ccagcagggc cgcctgtgtg
    28201 gcaaactgca tctgatgctt gcccgccgcc agcaccgacc attcataggc atcgccaaac
    28261 agcttctgcg cgatcgtgtg catatgcatc agattgtcga gatcggcgcc cactccaccg
    28321 aggatgccga acaccatctg cacgaagaac ggcgccttga tccagccctg gtcgatcacc
    28381 caggccagat tgtagaggtg gcccagatcg tagcattcga actcgaagcg cgtgccatgg
    28441 ccctcgccca gctccttgat gacatattcg atgtcggtga aggtgttgcg gaagatgaaa
    28501 tcgcgggtgc tttcaagaaa ggccggctcc cactcgtgct tccagttggt ctgcttggcg
    28561 cccatcggga agatgccgaa attcatcgag ccaacgttca gcgaggccat ttcggggctg
    28621 acgctgacag cggcgcgcag ccgttcctcg cggctcatgc caaggccgcc gccggtggag
    28681 acattcagca ccgcatcgct gtttcgggcg atctcgggca tgaactggcg gaacagctcc
    28741 gggcgcggat cgggcttgcc attctccggg ttgcgggcgt gcaggtggat gatcgcggcg
    28801 cccgcctcgg ccgcctcgat agaggccgtg gcgatctcgt cgggcgtgat cggcagatag
    28861 ggcgacatgg aaggggtgtg cacgccgccg gtgggcgcgc aggtgatgat aacggatttg
    28921 gccatggtgg ttctcacaaa tgcaagggcg gtggcaggcg ggcatccgcg ccgttcaggg
    28981 cgcgatggcg ccccgatagg catccagcac cagcccgtgg aaatggtgca gcgcatgttc
    29041 cgacaggccc gagccggcgg gatcggcaac gatgcgcccc tgatcgaaag cgggcgaatt
    29101 catgccgcgc tgcacattct cgacgatggc gatgtcttcg gcctgcagca cttcgtcgag
    29161 atagcggatc gcctccagct ccatcgcgtc ggcctcgggc gtttccagaa agaaatcgta
    29221 ggtttccagc gtcagatccg gccccaccgg gatgatgtgc agcacgatca tcgaactgcg
    29281 cccgggatag cgcatcaggc aggtgttggg ccacagccac cacaccgcat gggtgcgcac
    29341 cttggcggca gaggtatcgt aggcggaatt ggcgccatgg ccggcctcgg ccatatggct
    29401 ggaatagatc ccgtgggtgg tcaccttgta ggtgtccata tcgaccagcg tgacgaaatc
    29461 cctgtgcgag ctgggacagt ggtagcactc aaggaaattg tcgacgacat tcttccagtt
    29521 cgagcggatc gtgtaggtca gccggtggcc gaaagtgagc tggccgatat cgggcgccca
    29581 gccaaggatt tcctgcgcca gatcgccgga ggtttcgcgc agcgggcggg cctcgagatc
    29641 gagattgacg aagacgaagc cgcagaactc ctccacctgc actggctcca ggtgaatgtc
    29701 ggccttgctg aaattcttga ggtggcccac ctgcggggcg gccgcaaggc tgccatccag
    29761 cccatagcac cacgcatgat aggggcacac gatgcggctg accttgccct cgcccgccag
    29821 caccggatgg gcgcggtgtt tgcagacatt gtggaatgcc cgcaaaacgc catccttgcc
    29881 ccgcacaacc gcaaccggat tgccggcgat ggtggcagtt gtgtagctgc ccggatcacg
    29941 cagcttttcg acatggcaca cccattgcca cgatttggca atgatttcct gctgatcgat
    30001 atcgaaccag cgcccttgcg tataggcctc tgcgcgcaag gactgggtat ccagcggatc
    30061 tgtgctgtag cctttggcca catgatcgaa ctgatttgcg gttattacat gatgcatgag
    30121 aactgtcctc agccagggcc ggctcgatca accaggtcca ttctgtctga ggggaattat
    30181 gtctcggcgc gggcgtgaat atgttcccaa ttcgccataa atttgctcaa aatggccagc
    30241 aactcaggcg ctgtgccggt gcatcggcca ggcgcggaac tcgaagggaa tgcgcccctc
    30301 cagccggtcc tgccgcggcg acacgccaaa gcgcaggcga taggcccggc tgaaatgcga
    30361 ctggttgctg aagcccgacg caatgccgat ctcgaacagc gagaggctgg tctgcgttac
    30421 cagcccgcgc gcacgatcca ggcggatatc ccggtagtag gctgctggcg acttgccgac
    30481 gatatcgcca aacagccggt cgaggcttct ttgcgaaacg cccagctgtg tgcacaggtc
    30541 ggggatgcgg atggtctctt ccagatgccc ctccatcagg cgaatcgccc tgcgcacgat
    30601 cggcggcacg gcgctgccca ggggctcgcg gatatcgggg ctctggcggg tgtcgcaggg
    30661 gcgcaggctc tggtggaaca gatagtgcgc cgcctcgttg gccatggtcg cgccatgcag
    30721 ctggcagatg atgtgcagcg ccagatcgac agccgcgctg ccgccgcaac aggtcatgat
    30781 ccgctcgtcg atgacgatca tgtcctcgcg cacgtcgata tcgggataaa gctcggccag
    30841 ggcgtcgata tgctcataat ggcaggtggc gcggcgctgg tgcatcagcc ccgcctcggc
    30901 cagcaggaaa gcgccggtat ccagcgcgcc cagcgtcacc ccgccctgcg cccagccgcg
    30961 cagcatgtgg cgcagggccg gcgtgcaatg ccgctcgggc gaccagctcg acgagacgat
    31021 cgccagatcg atctcggccc ccttcaggct ggccagcggg ctggtggtaa tcgccagcga
    31081 attgctggcc atcacctccc cgccctcggc agacaccaca tcccagagga aatgctgccg
    31141 gcccgacaga tagttggtgg cccggaacgg atcgatgaag gccatggtgg cggccatgtt
    31201 gaagctgggc agcacgatga tggccatgcg catcggccgg ctgggctggc gctgcatggc
    31261 ggtttatgcg ggctggcgcg cgggctggcg cgcccactgg ccggcaatcg ccgacagctg
    31321 cgcccgcgcc ctgtccagcg ccgccagccg ggcttcggga tcggcaaact gcaccggctg
    31381 cgcatcgacg aaaagcggat ctgccacgcc gatgaagcca aaggccgccc gcagcgcggg
    31441 cgtcagcgca tccatcgccg caaagggcga gcccgggccc agatcggccc cacgcgtggt
    31501 gatgaacagc accggccgcc cgcccagctg gccttcatag cggccatcgg gcgtcatcac
    31561 ataggtgata ccggtgcgca cgatggcatc gatgaaggcc ttgaagttcg acggcatgct
    31621 ccagttgtac atcggcatgg caaagaccag cgcatcggcc gccagcagcc tttcgcacag
    31681 ccgatccgac ggggcgagca gcgcgtgcat ctcggccgtg cgggcctcgg ccggggtata
    31741 ggtggcgcgg gcgaaatcgc cggtcacatg cggcgggcaa tcatgcgcaa ggtcaagata
    31801 atccacctcc agcgggccgc cctgctccgc cagctgatcg acgaaatagc gcgacagcag
    31861 gcgcgaattc gagatctcgc ccttggggct gctgtccaca tgcatcagat tcatgcgttc
    31921 ttctccttgc gtttgcgggt gcgccgcgcg gcgcgggcat cgagccagcc accggcggca
    31981 gcgccaaaag cgggcggcgc cttggcgcgc agctggccgg ccagatcggc caaggtgcag
    32041 gcggccagcg cctcgtccag ccgcgcctgg gcggcgatca tcacggcgtg gatcgcgcag
    32101 gggccgcggg tggcccaatc cggcgcctga tcgccataaa gcacgcagcg ctggcggatt
    32161 tcgcggcatt cgaacagcgg cttgtcgcca tcgatcgccc gcacgacatc cagcacgcgg
    32221 atcgccgcgg gcgggcgcgc cagccgaacg ccgccgccga ccccctcgat cgagcgagtg
    32281 aggccggcct tttgcaggcg ggtgaacagc ttggcgagga attccggcgg caccgcctgc
    32341 agctcggcca gatcgcgcgt gctgggcgcc ggcgcctcct ccgcccggtc ggccaggaac
    32401 agcaggctgt gcagcgcata ttcgacgccg gtgctgatat gggcggccaa aactccgact
    32461 ccttaagtct gagttttaat agcgcggcat ccggcccgcc gcaaccctgc ccccgcctca
    32521 gcgcgccgaa cgcagatcgg ccgccaccga agcgatcgtc tcgcgcagcc agcgctgggc
    32581 cgcctgatcc tcaaagcggc gctgccacac cagcatctgg cgcagcggcg cgggctgcag
    32641 cggcagttcc accagtgcca cgggatgaac gcgggcgaac gccacggcga tctggcgcgc
    32701 catgatcgcc atcatgtcgg attgggtcaa cactgcgccg gccgacagat aaggcacctc
    32761 gatctcgctg ttgcgggcca ggccctgcct tgccagcgcc tcgtccaggc cggtcagatc
    32821 atcgccgctg gaggagatcg tcagatgcgc ctgggcggca aagccggcgc tatccagcgg
    32881 cgcgcccagc agcgggtgcc cctgccgcac caccgccacg aaatcatcct cgatcagcag
    32941 cgccgagcca taacgcgcca gcggcggcgc gcccgacacc agcgccagat ccagctcccc
    33001 ccggtccaac agatcggcga catcgagcgt gccactaggg cgcaggcgga tctgcacgcc
    33061 cggcgccagc gcccgggcgc gcagcacgat ggcgcctgcc agcgcaatcg ccgcataatt
    33121 gttgaccgcg atggcaaagc gctggcgggt gtacccgggc tcgaagggct cgtcctccag
    33181 cgcgctgcgc agatcgagca gggcccggcg caccggcagg g
//
LOCUS       DASRYE010000150        11056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_149905,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000150 DASRYE010000000
VERSION     DASRYE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149905"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFF98_08120"
     CDS             <1..217
                     /locus_tag="VFF98_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63634.1"
                     /translation="GVRGLPLADARRQVAALGPAAVPRQWAGAHAYVQRAIDLLAPAT
                     GRPRFDAATLFDPRFETIAADGMQEGN"
     gene            295..1092
                     /locus_tag="VFF98_08125"
     CDS             295..1092
                     /locus_tag="VFF98_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63635.1"
                     /translation="MAGRPAARNRFLAPRSVRGGLLLGPALLLLYWSVLSVNGWLDPR
                     VLPAPWTALDAAIELIRDGQLERHLAVSAGRALAGLALGAVAGVVLALLSGLSELGGY
                     VIDGLVQMKRGVPILALIPFMVLWLGIGEGMKISVIALAVFFPMYIQVHTALRAIDIR
                     YVELAETLRLSHAAFLLRIVLPGALPGLLTGLRFGTTAAWLALVVVEQLNATSGIGYM
                     VTLARDYAQTDVMLVGLVVYALLGFGSDLLVRALERHALRWRRSFGR"
     gene            1116..1820
                     /locus_tag="VFF98_08130"
     CDS             1116..1820
                     /locus_tag="VFF98_08130"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU63636.1"
                     /translation="MAAPAVSIRNLRRTFTLKGVLNGVDLDIAPGEFVALLGRSGSGK
                     STLLRALAGLDYEAVGSGAYQVPERTSVIFQDARLLPWLDILGNVTLGLGRAARDSAR
                     ATLAEVGLAGREHAWPHELSGGEQQRVALARALVRAPELLLADEPFGALDALTRGRMH
                     DLLRRLSDLHDPAVLLVTHDIDEAITLADRIVMLEDGRIATDIRVDLPAEPEPRAHRT
                     LALRAALLARLGPTPA"
     gene            1842..2657
                     /locus_tag="VFF98_08135"
     CDS             1842..2657
                     /locus_tag="VFF98_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU63637.1"
                     /translation="MTQPAERLAERYRQPADQPPGWNATIDALLAHRSVRAYLPDALA
                     PGTVELLVAAAQSAASSSNLQPWSVVAVEDPARKARLAALAGHQTHLEQAPLLLIWLV
                     DLNRLDTIGQQRGEPTEALAYLETFLLGAVDTALAAQNAVVAAESLGLGTVYVGAIRN
                     RPVEVAAELALAPQVFALFGLAVGHPDPARPAAVKPRLPQAAVLSREAYGNPQAPAAI
                     AAYDAHLEAFQREQGLPPRGWSAQATQRVGGPEAMAGRDRLREALATLGFALR"
     gene            complement(2665..3552)
                     /locus_tag="VFF98_08140"
     CDS             complement(2665..3552)
                     /locus_tag="VFF98_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZU63638.1"
                     /translation="MSGVLILDGGTGRELARIGAPFRQPEWSALALIEGPDYVSQVHR
                     AYIAAGADIITTNSYALVPFHIGEVRFAEQGAQLAALAGQLARAEADKAGGKVAVAGS
                     LPPVCGSYRPDLVDIARARPVLARLVAALAPYVDHWQAETLSALAEAELVAELVRPTG
                     LPLWLSFTLEDEAPGAVPTLRSGETVAEAAAAARRLGAAALLFNCSQPEVMAAAVAAA
                     RAELGDDAIRIGVYANAFPPQNAEAEANSGLDEIRADLTPAGYAAFASAWAGAGASIL
                     GGCCGIGPEHIAALKALKA"
     gene            complement(3549..5933)
                     /locus_tag="VFF98_08145"
     CDS             complement(3549..5933)
                     /locus_tag="VFF98_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63639.1"
                     /translation="MFTISTLSLPRLRGRSCLLAGVALSLGAPLVARAEEAPAPQADN
                     PASEIIVTGTRETGTRARNSATPIDVVGAKALNATGRVNLLDALKDILPSVNAPAVGY
                     DVGALARTFQLRGLSPDQTLVLVNGKRRHTSASIYADSDPAQGSNVVDLDLIPANAID
                     HVEVLRDGAAAQYGSDAIAGVVNVILKNGAAGGELGVQGGTYYKGDGANVAINVDHGV
                     KIGAEGALHLSAGYRLHDFSNRSGDSGGAQPAQVQGDPRSILFTGAYDYTQPLADHLT
                     LYSFGTWASRQARANENPREPGSISDAVDALYPNGFTPRETVNEHDFSVTAGLKGGTA
                     WAFDLSTTYGRNSSILHNVHTVNPDLLADTGNAQSVFYVGGYASSEWTSNLDIRRSFD
                     IGLAGPLTLAFGAENRYETYEIRAGEANSYYLGGASAFPGYRPSDATQAHRNSIAGYV
                     DATAHPLKGWELSLAGRVEHYDSVGSRVNGKVATRYDISPRLALRGSASTGFHAPSLA
                     QEYYSATTVTTGYASIQLPVNSAGARLLGAPALKPETSRNYSIGLVVEPVKGLHLSVD
                     VYRIDIDNRIIETEYLYGALAQAAVAANGSAIPAGLDPDNVAAQFFTNGVDTRTQGVD
                     VTATWRTDLGDWGTIGWNAGFGYVDTAIRRFHAAPAALQAAGLSLEDAVEISNITTAT
                     PHTKLSLAANWQRGPWAVTLRNTYYSNSTQYQGYASPYYIYTTGNRVITDLDVGYQLS
                     ARVRVGAGANNLFNIYPRRFGPEVYQNVNYDQYSHLSPFGINGGSYYLRLTVSL"
     gene            6140..7507
                     /locus_tag="VFF98_08150"
     CDS             6140..7507
                     /locus_tag="VFF98_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU63640.1"
                     /translation="MTHPSATPRRHLGVGQASLLALGMILSTDTLKTASTVALNVGHW
                     HFYGLWVLGGLISMAGALCYVEMAAAFPDAGGEYSFLRRAWGPQVGALFAWSRFAIMH
                     TGWIALMAYMFADYASALLHMGHTGQVLFAMGTVAALTALNLADVRLGFLTQALLVVL
                     VAAGFASVVAAGLVVAPAPALPDAGPRPPLGDISAALIYIFLAYGGWSDAATLSAEVR
                     ARRHGMLVASIGSIAMLIAIYVAINWALMRGLGANALAHSAAPAAQLCARAFGPAGAA
                     LIVGVVGISAIASINSTLIVGARTTYAAARDMPGLGFLGQWHPTRGTPARAVLAEGGF
                     ALLLTAAASLTESGFNTMVNYMTPVYWLFILLSMAAMIRLRLAHPEVERPVRTPLFPL
                     FPLGFMAMAGWMLYAAFTQLGAGALFGAGVLALGIVPNWLMQRRHPLRTASGHAGDGD
                     RQPAQ"
     gene            complement(7501..9711)
                     /locus_tag="VFF98_08155"
     CDS             complement(7501..9711)
                     /locus_tag="VFF98_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63641.1"
                     /translation="MNTVRLLVSSLALGTGLLAGVAAAEEAPAAPATADAPAGDIVVS
                     AQRRNESAQKVPLAITAISGDTLQQQTIDSAVDVAKFAPNVNAWNNDGRTRPRYYIRG
                     IGNGNVANNAVGAISVSQDEVYLNSLALQGFPLLDLERVEVLSGPQGTLQGKNSTAGA
                     INFISRKPEFVDTNGYANLSWGDYGQRQLETAAGGRIDDRFAWRAAARIERQGGLALN
                     RYDDTQVGDFTDIAARLQLRARLADGAVDVLASGHYRHLDATHTPFYAAIQGQSAPLG
                     IGNRNYDDSNLDDTQKLDSSGGFIRVDAHLPASLKLTSITSYDEGDRTEFLDGDYSTR
                     ELSRSYGRTKPEQLTQEIRLISPDGWFSWILGGYYFHERLNSFAATATLSNASGGTPG
                     YYYTQFTQYTNSAAVFGTTRFDLTKKLHLQGGLRWTSDDTSIHLVSQKATSPVSFSGT
                     SAWYLPGSTGQTLTDIARQNQSAHWNKLTWDVALRWEATRNISLYGRIANGYRAGNFQ
                     GQVAATTPPGLVNPETLTSYEAGVKTQWLGGKLVFNADVFHADYNAIQVSVVQSTVTG
                     INAQLANAGAGFSEGFEAELRAQPVSRLTLSGNLGLLRTRFTQFTISNATANINGNEF
                     ARAPHVTGFVNADYRIPLGHDSLTLGTDWHYTSRYYYIITDEKSPALQQAPVAVGNVR
                     VSYATHEDRVTFTLAVNNVAGATYTTQVLPYSYSSYGYTLGNPRTVLGSVRLRY"
     gene            complement(9843..10880)
                     /locus_tag="VFF98_08160"
     CDS             complement(9843..10880)
                     /locus_tag="VFF98_08160"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU63642.1"
                     /translation="MTALTYTRCPAVPTVSALAHRLGLIEEELRGEPGIEAAFASVGF
                     SPKVDYGHDDRFWLRNAGHAPALWKFAKGVKAKFVGLSFLEGRYPVLALASSGIGSVA
                     DLKGKRLALVGRRDAAFDLMVNQQLKIYRTALATAGLTLEDVTLVELDQSPPPVPASG
                     KPDFFSHLFRSRLALLEAGTVDAATAAIPPDAGHIPALNILYDTRQHPDPRERVNPSV
                     LRGLVVSEALIAERRDLVVRILARLLEAADWALAHPLEAIRLIAEDFDLPPERLAATY
                     ESLAEGIQLGFDAKRLEALAVQKDFLLEHGLIDRDIDLAEHIDTSLLEEARALYASRR
                     QAASQPLVSAG"
BASE COUNT         1688 a         3716 c         3792 g         1860 t
ORIGIN      
        1 gggcgtgcgc ggcctgccgc tggccgacgc ccgccgccag gtggccgccc tggggccagc
       61 tgccgtgccg cgccagtggg ctggcgccca tgcctatgtg cagcgggcga tagacctgct
      121 ggcgcccgcc accggccgcc cccgcttcga tgccgccacg ctgttcgatc cgcgtttcga
      181 aaccattgcc gccgatggca tgcaagaagg taactgatgg ccgcccttcc cgtttccgcg
      241 cccgccccgg cgcccccgcc tgcccgttcc ccgaccagtc gtccagtcaa tcgcgtggcc
      301 ggccgccctg ccgcgcgcaa ccgcttcctc gccccgcgat cggtgcgcgg tggcctgctg
      361 ctggggccgg cgctgctcct gctctactgg tcggtgctgt cggtcaacgg ctggctcgat
      421 ccgcgcgtgc tgcccgcgcc atggaccgcc cttgacgcag cgatcgagct gatccgcgat
      481 ggccagctgg aacgccacct tgccgtctcg gcggggcggg cgctggcggg gctggcgctg
      541 ggcgcggtgg caggggtggt gctggcgctg ctctcgggcc tcagcgagct gggcggctat
      601 gtgatcgacg ggctggtgca gatgaagcgc ggcgtgccca ttctggcgct gatccccttc
      661 atggtgctgt ggctgggcat tggcgagggc atgaagatca gcgtcatcgc gctggcggtg
      721 ttctttccca tgtatatcca ggtgcacacc gcgctgcggg cgatagacat ccgctatgtc
      781 gaactggccg agacgctgcg cctgtcgcat gcggcgttcc tgctgcggat cgtgctgccc
      841 ggcgccttgc cgggcctgct cacggggctg cgctttggca ccacggcggc ctggctggcg
      901 ctggtggtgg tggaacagct caacgccacc agcggcatcg gctatatggt cacgctggcg
      961 cgcgattatg cgcagacgga tgtgatgctg gtggggctgg tcgtctatgc gctgctgggc
     1021 ttcggctcgg atctgctggt gcgcgcgctg gaacgccatg ccctgcgctg gcggcggagc
     1081 tttggccggt gagcgcgctg gaaaccgcag cccagctggc cgcccctgcc gtcagcatcc
     1141 gcaacctcag gcgcactttc acgctgaaag gcgtgctcaa cggggtcgat ctcgatattg
     1201 cgccgggcga attcgtggcc cttctggggc gcagcggatc gggcaaatcc accctgctgc
     1261 gcgcgctcgc ggggctggat tacgaggcgg tgggctcggg cgcctatcag gtgccggaac
     1321 gcacctcggt gatcttccag gatgcccgcc tgctgccctg gctcgacatt ctgggcaacg
     1381 taacgctggg cctgggccgg gcggcgcgcg attctgcccg ggccaccttg gccgaagtgg
     1441 gccttgccgg gcgcgaacac gcctggccgc acgagctttc cggcggcgag caacaacgcg
     1501 tggcgctggc ccgcgccctt gtgcgcgccc ccgaactgct gctggccgac gaacccttcg
     1561 gcgcgctgga tgcgctgaca cgcgggcgca tgcatgatct gctgcgccgc ctgtcggacc
     1621 tgcacgaccc cgccgtgctg ctggtcaccc atgatatcga cgaggcgatt accctggccg
     1681 accgcatcgt catgctggaa gatggccgga tcgccacaga catccgggtt gatctgcccg
     1741 cagagcccga gccccgcgcc caccgcacgc ttgccctgcg cgccgcgctg ctcgcccggc
     1801 tggggcccac gcctgcctga acctcacacc ggagccctgc gatgacccaa cctgccgaac
     1861 gcctcgccga acgctatcgc cagccggcag accagccgcc cggctggaac gccacgatcg
     1921 atgcgctgct ggcccaccgt tcggtgcgcg cctacctgcc cgatgcgctg gcgcccggca
     1981 cggtggaact gctggtggca gccgcgcaat cggccgcctc ttcctccaac ctccagccgt
     2041 ggagcgtggt ggcggtggag gatccggccc gcaaggcccg gttggccgca ctggccggcc
     2101 accagaccca tctggaacag gcgccgctgc tgctgatctg gctggtcgat ctcaaccggc
     2161 tcgataccat cggccagcag cgtggtgagc ccacagaagc gctggcctat ctcgaaacct
     2221 tcctgctggg cgcggtagac actgcgctgg cggcgcaaaa tgccgtggtt gcggcagaat
     2281 cgctggggct gggcacggtc tatgtcggcg ccatccgcaa ccggccggtg gaagtggcgg
     2341 cagagctggc cctggcgccg caggtgtttg ccctgtttgg cctggccgtg ggccatcccg
     2401 atccggcgcg gccggccgcg gtgaaacccc gcctgccgca ggccgcagtc ctctcgcgcg
     2461 aggcctatgg gaacccgcag gcgcccgcgg ccatcgccgc ctatgacgcc cacctcgaag
     2521 ccttccagcg cgagcagggc ctgcccccgc gcggctggag cgcgcaggcc acccagcgcg
     2581 tgggcgggcc agaagccatg gccggccgcg accggctgcg cgaagccctg gcgacgctgg
     2641 gcttcgcgct cagataacgc ccaatcaggc cttcagggcc ttcagcgcgg cgatatgttc
     2701 cgggccaatg ccgcagcacc cgcccagaat gctggcccct gcccccgccc aggcgctggc
     2761 aaaggcggca tagccggccg gcgtgagatc ggcgcggatc tcgtcgaggc cggaattggc
     2821 ttccgcctcg gcgttctgcg gcgggaaggc attggcataa acgccgatgc ggatggcatc
     2881 atcgcccagc tctgcccgcg cggcggcaac cgctgcggcc atcacctcgg gctggctgca
     2941 attgaacagc agcgctgcgg cccccagccg gcgggcggca gcggcggcct cggccaccgt
     3001 ctcgcccgaa cgcagggtgg gcaccgcgcc gggtgcctca tcctccagcg tgaaggacag
     3061 ccacagcggc aggccggtgg ggcgcaccag ttcggccacc agctccgcct cggccagcgc
     3121 cgagagggtt tcggcctgcc agtggtccac atagggcgcc agcgccgcca ccagcctggc
     3181 cagcaccggc cgcgcccttg ctatatccac cagatcgggc cgataggaac cgcacaccgg
     3241 cggcaacgat ccggccacgg ccactttgcc gccggcctta tccgcctcgg cccgggccag
     3301 ctggccggcc agcgcggcca gctgcgcgcc ctgttcggca aagcgcacct cgccgatgtg
     3361 gaagggcacc agcgcatagc tgttggtggt gatgatatcg gcgccggccg cgatataggc
     3421 gcggtgcacc tggctcacat aatccggccc ctcgatcagc gccagcgccg accattcagg
     3481 ctggcggaag ggcgcgccga tgcgggccag ctcgcgcccg gtgccgccat cgaggatcag
     3541 cacgccgctc acaggctcac cgtcaggcgc agatagtagc tgccgccgtt gatgccgaaa
     3601 ggcgagaggt gggaatactg gtcgtagttc acgttctgat agacctcggg gccaaagcgg
     3661 cggggataga tgttgaagag gttgttggcc cctgccccca cacgcacccg cgccgacaac
     3721 tgataaccca catcgagatc ggtgatcacc cggttgccgg tggtgtagat gtagtagggc
     3781 gaggcatagc cctgatattg cgtggaattg ctgtaatagg tgttgcgcag ggtcaccgcc
     3841 caggggccgc gctgccagtt ggccgccagc gacagcttgg tgtgcggcgt ggcggtggtg
     3901 atgttgctga tctccaccgc atcctccagc gacaggccgg ccgcctgcag ggcggcgggc
     3961 gcggcgtgga agcggcggat cgcggtgtcc acataaccaa agcccgcgtt ccagccgatc
     4021 gtgccccaat cgcccagatc ggtgcgccag gtcgccgtca cgtccacgcc ctgggtgcgg
     4081 gtgtcaacgc cattggtgaa gaactgggcg gccacattat ccggatccag ccccgcggga
     4141 atggccgagc cattggccgc cacggccgcc tgcgccagcg cgccataaag atattcggtc
     4201 tcgatgatgc ggttgtcgat gtcgatgcga tagacatcga ccgacagatg cagccccttc
     4261 accggctcta ccaccaggcc gatgctgtag ttgcgcgagg tttccggctt cagcgccggc
     4321 gcccccagca gccgcgcgcc tgccgaattg accggcagct ggatggaggc atagccggtg
     4381 gtgacggtgg tggccgaata atattcctgc gccaggctgg gcgcgtggaa gccggtgctg
     4441 gcgctgccgc gcagggccag gcgcgggctg atgtcgtagc gggtggccac cttgccgttc
     4501 acccggctgc ccacgctgtc gtaatgttcc acgcggccgg ccaggctcag ctcccagccc
     4561 ttcagcgggt gggccgtggc atccacatag ccggcgatgg aattgcgatg ggcctgcgtg
     4621 gcatcgctgg gccgatagcc ggggaaggca gacgccccgc ccagataata gctgttcgcc
     4681 tcgcccgcgc ggatctcata ggtttcgtag cggttctccg cgccgaaggc cagggtcagc
     4741 gggccagcca ggccaatgtc gaagctgcgg cggatgtcga ggttgctggt ccattcgctg
     4801 ctggcatagc cgccgacata gaacacgctc tgcgcattgc cggtatcggc cagcagatcc
     4861 gggttcaccg tgtgcacatt gtgcaggatg gaactgttgc ggccataggt ggtggagagg
     4921 tcgaacgccc aggcggtgcc gcccttcagc ccggccgtca cgctgaaatc atgctcgttg
     4981 actgtctcgc gcggggtgaa gccattggga tagagcgcat ccaccgcatc ggaaatgctg
     5041 cccggctcgc gcgggttctc gttggcgcgg gcctggcgcg aggcccaggt gccaaagctg
     5101 tagagcgtga ggtgatcagc caggggctgg gtataatcgt aggcgccggt gaacaggatc
     5161 gagcgcggat cgccctgcac ctgcgccggc tgggcgccgc cggaatcgcc gctgcggttg
     5221 gaaaaatcat gcaggcgata gccggccgag agatgcagcg ccccttcggc gccaatcttg
     5281 acgccatgat cgacattgat cgccacattg gcgccatcgc ccttgtagta ggtgccgccc
     5341 tgcacgccca gctcgccgcc cgcagcgccg ttcttgagga tgacattgac gacgccggca
     5401 atcgcatccg atccatattg cgccgccgcg ccatcgcgca gcacttccac atggtcgatc
     5461 gcattggccg ggataagatc gagatcgacc acattcgagc cctgcgccgg atcactgtcg
     5521 gcatagatgc tggccgatgt gtggcgccgc ttgccgttca ccagcaccag cgtctgatcg
     5581 gggctgaggc cgcgcagctg gaaggtgcgg gccagcgccc ccacgtcata gcccaccgcg
     5641 ggcgcgttca ccgagggcag gatatccttc agcgcatcca gcaggttcac ccgcccggtg
     5701 gcattcagcg ccttggcgcc caccacatcg attggcgttg cgctgttgcg ggcgcgggtg
     5761 ccggtctcgc gcgtgccggt aacgatgatt tcagacgcgg gattgtcggc ctgaggcgcc
     5821 ggcgcttctt cggcccgcgc caccagcggc gccccaaggc tcagggcaac accggccagc
     5881 aagcaggaac ggccacgcag gcgcggcagc gaaagggtag agatggtgaa caagcggccc
     5941 cctgaaaagg tctaaactct agtgttttta tggactataa aacgcaggcc attcgcgcca
     6001 tgcagttttt ctagggcgcc cgatagcgtg gctggcccgc agatcaaccc cgccgccggt
     6061 gttttgccct cttgcacgcc accctgcccg cctgatagcc acgccgtttc ccctcgcacc
     6121 cggaagacca gcccccaccg tgacccaccc ttccgccacg ccgcgccgcc atctgggcgt
     6181 gggccaggcc agcttgctgg cgctgggcat gatcctgtct accgacacgc tgaaaaccgc
     6241 ctccaccgtg gcgctgaatg tgggccactg gcatttctat ggcctgtggg tgctgggcgg
     6301 gctgatctcg atggcaggcg cgctgtgcta tgtggaaatg gccgccgcct tccccgatgc
     6361 cggcggcgaa tacagcttcc tgcgccgcgc ctgggggccg caggtgggcg cgctgttcgc
     6421 ctggtcgcgc tttgccatca tgcacaccgg ctggatcgcg ctgatggcct atatgtttgc
     6481 cgattatgcc agcgccctgc tgcatatggg ccatacaggg caggtcttgt tcgccatggg
     6541 cacggtggcg gcgctgacgg cgctgaacct cgccgatgtg cggctgggct ttctcaccca
     6601 ggcgctgctg gtggtgctgg tggcagccgg ctttgccagc gtggtggccg ccgggctggt
     6661 ggtggcgcct gcccccgccc tgcccgatgc cggcccgcgc ccgccgctgg gcgatatttc
     6721 ggccgcgctg atctacatct tcctggccta tggcggctgg agcgatgccg ccaccctttc
     6781 ggccgaggtg cgcgccaggc gccatggcat gctggtggcc agcatcggct cgatcgccat
     6841 gctgatcgcc atctatgtgg cgatcaactg ggcgctgatg cgcgggctgg gcgccaacgc
     6901 gctggcccat agcgcggcgc ccgcggcgca attgtgcgcc cgcgcctttg gcccggcggg
     6961 cgcggcgctg atcgttggcg tggtgggcat ttcggccatc gccagcatca attccacgct
     7021 gatcgttggc gcgcgcacca cctatgccgc cgcgcgcgat atgcccggcc ttggctttct
     7081 gggccagtgg caccccacgc gcggcacgcc ggcccgcgcg gtgctggcag aaggcggctt
     7141 tgccctgctg ctgacggccg ccgccagcct caccgaaagc ggcttcaaca ccatggtgaa
     7201 ctacatgacg ccggtctact ggctgttcat cctgctttcc atggcggcga tgatccgcct
     7261 gcgcctggcc caccctgaag tggaacggcc ggtgcgcacg ccgctgtttc cgctgtttcc
     7321 gctggggttc atggcgatgg ccgggtggat gctctatgcc gccttcaccc agctgggcgc
     7381 cggcgcgctg tttggtgccg gcgtgctggc gctgggcatc gtgcccaact ggctgatgca
     7441 gcgccgccat cccttgcgca ccgccagcgg ccacgcaggg gatggcgatc gccagcccgc
     7501 tcagtagcgc aggcgcaccg agcccagcac cgtgcgcgga ttgcccagcg tgtagccata
     7561 gctgctatag ctgtagggca gcacctgcgt ggtgtaggtg gcgccggcca cattgttgac
     7621 cgccagcgtg aaggtcaccc tgtcctcatg ggtggcatag ctgacccgca cattgcccac
     7681 cgccaccggc gcctgctgca gggcgggcga tttctcgtcg gtgatgatgt agtagtagcg
     7741 gctggtatag tgccagtcgg tgccaagggt caggctgtcg tggcccagcg ggatgcggta
     7801 atcggcgttg acgaagccgg tgacatgcgg cgcgcgggca aattcattgc cgttgatgtt
     7861 ggccgtggcg ttcgagatgg tgaactgggt gaagcgcgtg cgcaacaggc ccagattgcc
     7921 gctcaacgtc agccgcgaga cgggctgggc gcgcaattcg gcctcgaagc cttccgaaaa
     7981 gccagcgccg gcattggcca gctgggcgtt gatgccggtg acggtggatt gcaccaccga
     8041 gacctggatg gcgttgtagt cggcatggaa gacatcggcg ttgaacacca gcttgccgcc
     8101 cagccactgc gtcttcacgc cggcctcgta ggaggtcagc gtttccgggt tcaccagccc
     8161 cggcggcgtg gtggccgcca cctgcccctg gaaattgccc gcccgatagc cattggcaat
     8221 gcggccataa agcgagatgt tgcgggtggc ctcccagcgc agcgccacat cccaggtcag
     8281 cttgttccag tgggcgctct ggttctggcg ggcgatgtcg gtcagcgtct ggccggtgct
     8341 gcccggcaga taccaggccg aagtgccgct gaagctgacc ggcgaagtgg ccttctggct
     8401 gaccaggtgg atcgaggtat cgtccgaggt ccagcgcagg ccgccctgca ggtgcagctt
     8461 cttggtcaga tcgaagcggg tggtgccgaa gacggcggcg ctgttcgtat attgggtgaa
     8521 ctgggtgtaa taatagccgg gcgtgccgcc gctggcattg ctgagggtgg ccgtggcggc
     8581 gaaggaattg agccgctcgt ggaagtaata accgcccagg atccacgaga accagccatc
     8641 gggcgagatc aggcggatct cctgcgtcag ctgctcgggc ttggtgcggc cataggagcg
     8701 cgacagttcg cgcgtgctgt agtcgccatc gaggaattcg gtgcggtcgc cctcgtcgta
     8761 ggacgtgatc gaggtcagct tgagcgaggc gggcagatgc gcatcgacgc ggataaagcc
     8821 gccgctggaa tcgagcttct gggtatcgtc gaggttgctg tcgtcataat tgcggttgcc
     8881 gatgcccagc ggcgccgact ggccctgaat ggccgcatag aagggcgtgt gggtggcatc
     8941 gaggtggcga taatggccgc tggccagcac atccaccgcg ccatcggcaa ggcgcgcgcg
     9001 caattgcagg cgcgcggcaa tatcggtgaa atcgcccacc tgcgtatcgt catagcggtt
     9061 gagcgcaagg ccaccctgcc gctcgatgcg ggccgccgcg cgccaggcga agcgatcatc
     9121 gatcctgccg ccggccgcgg tttccagctg gcgctggcca taatcgcccc acgacaggtt
     9181 ggcgtagccg ttagtgtcga cgaattcggg cttgcgcgag atgaagttga tggcgccggc
     9241 ggtggaattc ttgccctgca gcgtgccctg cggcccgctc agcacttcca cccgctccag
     9301 atccagcagc gggaagccct gcagcgccag gctgttgaga tagacctcat cctgcgacac
     9361 gctgatcgcg cccaccgcat tgttggccac attgccgttg ccgatgccgc ggatatagta
     9421 gcgcgggcga gtgcggccat cattgttcca ggcgttcaca ttgggcgcga atttggcgac
     9481 atcgacggcg ctgtcgatgg tctgctgctg cagcgtgtcg ccgctgatgg cggtgattgc
     9541 cagcggcact ttctgcgcgc tttcattgcg gcgctgggcc gagacgacga tatcgccagc
     9601 gggcgcatcg gcggtggcgg gcgcggccgg cgcctcttcg gcagcggcca caccggccag
     9661 cagccccgtg cccagcgcca gcgaggacac cagcaaacga acagtattca tgataaaaac
     9721 cccctgaaca ggcataaaac ggcccccgcc ggagaagcga tgtctccggc atcgggcatg
     9781 gaaaaaggga tgggtgggtg cgggcgggcc ggcccgcacc ctgtgtcagc cggccgaaac
     9841 catcagccgg cggaaaccag cggctggctg gctgcctgcc ggcgcgaggc atagagcgcg
     9901 cgcgcctctt ccagcaggct ggtgtcgata tgctcggcca gatcgatatc gcggtcgatc
     9961 agcccgtgtt cgagcaggaa atccttctgc accgccagtg cctccagccg cttggcatcg
    10021 aagcccagct ggatgccctc ggccaggctc tcataggtgg ccgccaggcg ctccggcggc
    10081 aggtcgaaat cctcggcgat caggcggatc gcttccagcg gatgggccag cgcccaatcg
    10141 gccgcttcca gcagccgcgc cagaatgcgc accaccagat cgcgcctttc ggcaatcagc
    10201 gcttccgaca ccaccagccc gcgcagcacg gaagggttca cccgttcgcg cggatcggga
    10261 tgctggcggg tgtcgtagag gatgttgagc gcggggatat ggccggcatc gggcgggatg
    10321 gcggccgtgg cggcatccac cgtgcccgct tccagcaggg cgaggcggct gcggaagagg
    10381 tggctgaaga aatcgggctt gccagaggcg ggcaccggcg gcgggctctg gtccagctcc
    10441 accagcgtca catcctccag tgtgaggccg gccgtggcca gcgccgtgcg atagatcttc
    10501 agctgctggt tcaccatcag gtcaaaggcg gcatcgcgcc ggcccaccag cgccagccgc
    10561 ttgcccttca gatccgccac cgagccgatc cccgaggaag ccagcgccag caccggatag
    10621 cgcccttcga ggaaggagag gccgacgaat ttggccttca cccccttggc gaatttccac
    10681 agggccgggg catggccggc attgcgcagc cagaagcggt cgtcgtggcc ataatccacc
    10741 ttgggcgaga aacccaccga ggcgaaagcc gcctcgatgc cgggctcgcc gcgcaattcc
    10801 tcctcgatca gccccagccg gtgggccagc gccgaaacgg tgggcaccgc cgggcagcgg
    10861 gtataggtca atgcggtcat gggatcagat cctttttcag gccagcgccg gctggggcgc
    10921 ggcatcgagg cccggaatgg caaagcgggc gagcaactgg cgggcattgc cggcaaacac
    10981 ggcatgggcc tcctcggcgc tggtcacggc cgcctcgata ttggcgatga cggggggaat
    11041 ggcgggcacc ggcgcg
//
LOCUS       DASRYE010000151         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_8645,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000151 DASRYE010000000
VERSION     DASRYE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8645"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFF98_08165"
     CDS             <1..421
                     /locus_tag="VFF98_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZU63643.1"
                     /translation="GAGLVAADDPSNRNDAFDRARLRARLAGVDWLDIPALAQSAAHI
                     AEADAALEWAAAREWREAVTQTGLALTYAPRSPRAIGLRVVARIVARLDGEEARGSAI
                     ARLYDRLNAGQTASIGGLVVRPGPDGWAFMKAPSRRS"
     gene            complement(422..1237)
                     /locus_tag="VFF98_08170"
     CDS             complement(422..1237)
                     /locus_tag="VFF98_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZU63644.1"
                     /translation="MTTRNLPLVPLRAVPAVQAGVRPMPFGRQEWPQGHAHARHHAAR
                     SHRRGPARGGQRGGWLALAVLAALALALVWLGASAAAAAEPAHIAPLPLDEQTGSFAP
                     ESAPAPGRPAAPARRQPFTIKRILPITEPIRYGQWFWDERGVPAGPIVVTVDLDARVL
                     SIFRGGYEIGTTAVLLGSQEKPTPLGIFPITQKQVTHFSNIYGGAPMPYMQRLTNDGV
                     TIHATSVANGYASHGCVGVPLDFAKKLYATTHLGDRVYITRGKQVGLGDSLTG"
     gene            complement(1324..>3275)
                     /locus_tag="VFF98_08175"
     CDS             complement(1324..>3275)
                     /locus_tag="VFF98_08175"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZU63645.1"
                     /translation="EAHPDKLVSLAPLMAELEALAARAGEGPAAQIQIAIAAMQADGL
                     GMGWVHFRVNSSQLQNAIRRRIDPDGTLDLASQAALMKMRELLAQVQPLRSNFAALAI
                     ENSTAVRLFLTMAQILRHIDADSPIRMLVAECEQPTTVLAALYFAKLFGVEGKVDVSP
                     LFETETALEHGGRFLDALLAEPAYRTYARTRGRVSIQTGFSDAGRFVGQIPAALAIER
                     LQGRLAQAMAANDLGDVSALIFNTHGESMGRGAHPASMADRLAWPLSPWARRRFVRAG
                     IRLEPEVSFQGGDGYLFFGTEELALATLTRFIELAPAETDPYAPTDPFYRRTDLSLDF
                     YRAIRRVQQRHLASGTYSRAVTAFGLGLLNDTGSRKSRRQSDLAADRNMSLRQIRAIP
                     HNAVLQQLGYPVNIIAGFGTAADGNAEEIASLLASSERGRQMLRLVRAANTLASIKTV
                     SAFGELFNSGYWASRPYRGHEDHIEDACLHLAEYLTSDDRAGVFRRLATRLRVDALKL
                     HRFLALIPEGADAGLSTHDRERNRRMIGVLQALRLALFQHMFLKVVQVPAFSRANDIA
                     REDVLEMVFTLRVDEALAQLRRAFPTSFPSLADFTVDEPADYPDEAATGYAAIQRAYI
                     EPIEQAYGLALRIGTAIANEFGAHG"
BASE COUNT          534 a         1090 c         1151 g          500 t
ORIGIN      
        1 cggggccggg ctggtggcgg ccgatgatcc ttccaaccgc aacgacgcgt ttgaccgggc
       61 ccgcctgcgc gcgcgcctcg ccggggtgga ctggctggat atccccgccc ttgcccaaag
      121 cgccgcccat atcgccgagg ccgatgccgc gctggaatgg gcggccgccc gcgaatggcg
      181 cgaggcggtg acgcaaacgg ggctggccct cacctatgcg ccgcgcagcc cgcgcgcgat
      241 tggcctgcgc gtggtggccc gcatcgtcgc gcggctggat ggcgaggagg cgcgcggcag
      301 cgccatcgcc cgcctgtatg acaggctgaa cgccggacag accgcctcta tcggcgggtt
      361 ggtggtgcgg ccggggccgg atggctgggc cttcatgaaa gcacccagcc gacgcagctg
      421 atcaaccggt gagcgaatcg cccagcccca cctgcttgcc ccgggtgata tacaccctgt
      481 cgcccagatg ggtggtggca tagagctttt tggcgaaatc cagcggcacc cccacgcagc
      541 catggctggc atagccattg gccacgctgg tggcatggat cgtcaccccg tcattggtga
      601 ggcgctgcat atagggcatt ggcgcgccgc catagatgtt cgagaaatgc gtgacctgct
      661 tctgggtgat ggggaagatg cccagcggcg tgggcttttc ctggctgccc agcagcacgg
      721 cggtagtgcc gatctcatag ccgccgcgaa agatcgacag cacccgcgca tcgagatcga
      781 ccgtgacaac gatgggcccc gccggcacgc cccgttcatc ccagaaccac tggccatagc
      841 ggatcggctc ggtgatcggc aggatgcgct tgatggtgaa gggctggcgg cgcgcggggg
      901 cggccgggcg cccgggcgca ggggcgcttt ccggcgcgaa gctgccggtc tgctcgtcca
      961 gcggcagggg ggcgatatgg gccggctctg ccgctgcggc cgcgcttgcc cccagccaca
     1021 ccagcgccag agccagcgcc gccagcaccg ccagcgccag ccagcccccc ctctgcccgc
     1081 cccgcgccgg gccgcggcgg tggcttctgg cggcatgatg gcgcgcatgg gcgtggccct
     1141 gcggccattc ctgccggcca aagggcatgg ggcgcacccc ggcctgcacg gccggcaccg
     1201 cgcgcagggg gacaagcggg aggttcctgg tggtcatacc taggtctata ccacgcaagc
     1261 cttgccgttt gcctaaaatg gcaacctgtt gccggtgcga caaacagggt taatatcgct
     1321 taatcagcca tgcgcgccaa attcattggc gattgccgtg ccaatgcgca gggccaaccc
     1381 ataggcctgc tcgatcggct cgatataggc gcgctggatc gccgcatagc cggtggcggc
     1441 ctcatccggg taatcggccg gctcgtccac ggtgaaatcg gcaaggctgg ggaagctggt
     1501 gggaaaggcc cggcgcagtt gcgccagcgc ttcatccacg cgcagggtga agaccatctc
     1561 cagcacatcc tcgcgggcga tatcattggc gcggctgaag gcgggcacct gcaccacctt
     1621 caggaacatg tgctggaaca gcgccaggcg cagcgcctgc agcacgccga tcatccgccg
     1681 gttgcgctcg cggtcgtggg tggaaaggcc cgcatcggcg ccttccggga tcagcgcgag
     1741 gaagcggtgc agcttcagcg catccaccct gaggcgcgtg gccaggcggc ggaacacgcc
     1801 ggcgcggtcg tcggaagtga gatattcggc cagatgcagg caggcatcct cgatatggtc
     1861 ctcatgcccg cgataggggc ggctggccca atagccggaa ttgaacagct cgccaaaggc
     1921 gctcaccgtc ttgatgctgg ccagcgtatt ggccgcgcgc accagccgca gcatctgccg
     1981 cccgcgctcg ctgctggcca gcaggctggc gatttcctcg gcattgccat cggccgcggt
     2041 gccaaagccg gcgatgatgt tcaccggata gcccagctgc tgcagcacgg cattgtgggg
     2101 gatggcgcgg atctggcgca ggctcatgtt gcggtcggcg gcaaggtcgc tctggcggcg
     2161 gctcttgcgg ctgccggtgt cgttcagcag gcccaggcca aaggcggtga cagcgcgcga
     2221 ataggtgccg ctggccagat gccgctgctg cacccggcgg atggcacgat agaaatcgag
     2281 cgagagatcg gtgcggcgat agaagggatc ggtcggcgca taggggtcgg tctcggccgg
     2341 ggccagctcg atgaagcggg tcagcgtggc cagcgccagt tcctcggtgc cgaagaagag
     2401 atagccatcg cccccctgga acgacacttc cggctccagc cggatgcccg cgcgcacaaa
     2461 gcgccgccgc gcccagggcg acagtggcca ggccagccga tcggccatgg aggccggatg
     2521 ggcgccgcgg cccatgctct cgccatgggt gttgaagatc aacgccgaca catcgcccag
     2581 atcattggcc gccatcgcct gggccagccg cccctgcagc cgctcgatcg ccagcgccgc
     2641 cgggatctgg ccgacaaagc ggccggcatc ggaaaagccg gtctggatcg acacgcggcc
     2701 gcgcgtgcgg gcataggtgc gataggccgg ctctgccagc agcgcatcga ggaagcggcc
     2761 gccatgttcc agcgcggttt ccgtctcgaa cagcggcgag acatcgacct tgccttccac
     2821 cccgaacagc ttggcgaaat agagcgcggc cagcaccgtc gtcggctgct cgcattcggc
     2881 caccagcatg cggatcgggc tgtcggcatc gatatgccgc aggatctgcg ccatggtgag
     2941 gaacaggcgc acggcggtgg aattctcgat ggcgagcgcg gcaaaattgc tgcgcagcgg
     3001 ctgcacctgg gccagcagtt cgcgcatttt catcagcgcg gcctggctgg ccagatccag
     3061 cgtgccatcg ggatcgatgc ggcggcggat agcgttttgc agctgcgagc tgttcacccg
     3121 gaaatgcacc cagcccatgc ccagcccatc ggcctgcatg gcggcgatgg cgatctggat
     3181 ctgggcggcg gggccttccc cggcgcgggc ggccagcgct tccagctcgg ccatcagcgg
     3241 ggccaggctg acgagcttgt cgggatgggc ttccg
//
LOCUS       DASRYE010000152         7993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_203218,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000152 DASRYE010000000
VERSION     DASRYE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203218"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFF98_08180"
     CDS             <1..792
                     /locus_tag="VFF98_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZU63646.1"
                     /translation="HIEQDGPAIGGNRPIDVALAGDLAAILPQLSGRLARAAGADLAR
                     WSAAEAARRAALAQGPSAGAGPLHPARLVVEATRALPQEAILVRDGGAFVLFQLAYGQ
                     GHPRDVLWSQNLAHLGTGLPYAIGAAQATGGQRPVVLLTSDSALLFHIAELETAARLG
                     LPILCVVAVDHQWGLEAGMYRRLHGPATPEPGAHWSRDVRFDRIARGLACPASQVTQA
                     QEIAPAMAAALAAGGPYLLHVPIDAAANAGEIPAFAEFASWYAQA"
     gene            complement(805..1236)
                     /locus_tag="VFF98_08185"
     CDS             complement(805..1236)
                     /locus_tag="VFF98_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63647.1"
                     /translation="MRKLPASCLAMLALAGIGCTAAPATARERLTGEQQLAKMLQGRQ
                     AGAPVDCVSIISLGSSTVIDKTAIVYDSGSVLYVNRTDAPDQLASDDILVTKTSLDQL
                     CHLDIMQLRDRAGGFYRGSVGLGQFVPWTRVPKDGGAKSGH"
     gene            complement(1315..2754)
                     /gene="tldD"
                     /locus_tag="VFF98_08190"
     CDS             complement(1315..2754)
                     /gene="tldD"
                     /locus_tag="VFF98_08190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZU63648.1"
                     /translation="MTLATDPRSLLYTPGQLAPDEAQKLVGSALAACEDGELFLQFTA
                     SEAFGFDDGRLKTADYSREAGFGLRGVSGETTGFAHANEISAAAIRRAGETLTLLDPA
                     QGTRPAPPARTNRHLYTDTSPLDLVPFAKKVALLEAIDAAARARDPRVAQVSASLSAS
                     WSVVEILRADNFGGAGPVLDVRPLVRLNVSIVAEQNGRRETGSFGMGGRWLYDRLFEP
                     ESWNRAIDTALAQALTNLESVAAPAGEMQVLLAPGWPGVLLHEAVGHGLEGDFNRKGT
                     SAFSGRIGERVAAPGVTVVDDGSLAGRRGSLSVDDEGTPTQENVLIEDGILKGYIQDR
                     LNARLMGVAPTGNGRRESYAHAPMPRMTNTFMRAGNDDPAELLSRMKNGIFAKSFGGG
                     QVDITSGKFVFSCTEAYLVENGKLGAPIKGATLIGDGPSVLTRVTGIGHDLALDEGVG
                     VCGKGGQSVPAGVGQPTLLVEGLTVGGTG"
     gene            complement(2858..3058)
                     /locus_tag="VFF98_08195"
     CDS             complement(2858..3058)
                     /locus_tag="VFF98_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZU63649.1"
                     /translation="MTQQPETVTVTSTRVACDGAGEIRSTGNYAPQALGHPRIWLEID
                     EKGFVDCPYCDKRFVLDAHHAH"
     gene            3405..4667
                     /locus_tag="VFF98_08200"
     CDS             3405..4667
                     /locus_tag="VFF98_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU63650.1"
                     /translation="MVIANRELLICDLTQSYAPRGGGGVSTYLSEKTRHVLSKTSHRL
                     LQIVPGERDRIVEKGRHIWVEVGADQVRGSPNYRFILRTGAVREILERYRPDIIESQC
                     PWVLPWTAINYRRAHPETALVAGYHTDFPNVHVHRVGSQIFGETLGGILRRISIGYAT
                     FTYREFDRVYAMNEQMREQLAGYGIRHVDVLTLGVDTDQFNPARRDPTYRARMGLAGH
                     GPLLAYAGRLDNEKRADRLVEMLRELPRDMGAALVMFGDGKLREKIMSIAQAENLPVA
                     LPGFVDDRDALAVALASSDIYVSAMADETFGISVIEAQASGLPVVGVASGAMPARVPQ
                     GLGLLGPVDDVVTMAANVQQIWANDPRAIGAAARAHAARRYSWEKTFSYLIGEVYPTA
                     LESAARRVHSPRAWKISARAASPLRGAA"
     gene            4728..5687
                     /locus_tag="VFF98_08205"
     CDS             4728..5687
                     /locus_tag="VFF98_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU63651.1"
                     /translation="MSASSSLPPAISIRNLVKRYAGSAGAPGKLALGGVSFDVPQGQI
                     FGLLGPNGAGKSTLINTLAGLVMKTSGEVDIWGFDIDRDRRNASRAIGIVPQEIVFDP
                     FFTPFEVLEIQAGLYGVPRAQRRTDELLAAVHLSDKARAYARTLSGGMKRRLLVAKAM
                     VHQPPVLVLDEPTAGVDVELRRQLWELVKGLNDEGVTVVLTTHYLEEAEELCDRIAII
                     NHGQLIANKPTRELVGMAREKVVVLTVDREVSAPPAHPAFIKAQVTAPRTIEISYDKD
                     RATAGEILGLVQAQGLGIVDVSTREADLEDVFVALTSERPQAG"
     gene            complement(5739..5957)
                     /locus_tag="VFF98_08210"
     CDS             complement(5739..5957)
                     /locus_tag="VFF98_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63652.1"
                     /translation="MALRLPLCLFNRHAPDRNRVKWDGLHFIGKCRFCGKPIRRRDKG
                     RWEKDWLTDTGKAGRLIEDQRENGHKGS"
     gene            6291..7880
                     /gene="nadB"
                     /locus_tag="VFF98_08215"
     CDS             6291..7880
                     /gene="nadB"
                     /locus_tag="VFF98_08215"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZU63653.1"
                     /translation="MSGAAPSADVIVVGSGAAGLTAALALAERLKVLVVAKGSLTGGS
                     TAWAQGGIAAVLDAGDTFEDHIRDTMVAGAGLNRIETVEHVVERAPHAIDRLIDLGVP
                     FNTEGNNLHLTREGGHSHRRIVHVDDATGWAVQSALLKAAEAHPNITLLPGRACIDLI
                     TGRHELRYSGSGRVWGVYALDEASGRVEAHTARATILATGGAGRCYLYSTAPRGATGD
                     GIAMAWRAGARVANMEMMQFHPTCLYNLDVKNFLITEAVRGEGGRLINPRTAKRFMPF
                     YDERLELAPRDVVARAIDSEIKKFGLDYVHLDISHMPSDFVAEHFPNIREKLAGLGID
                     MTKQPIPVVPAQHYTCGGIVTDLHGRTDLPGLYAAGECSESGLHGANRLASNSLLECF
                     VFGEAAAADILANWADFAPPPQVRAWDESRATDSDEEVIIKQNWTEIRRFMWNYVGIV
                     RTTKRLERAQHRINMLFGEVEEYYSAFRVTTDLIELRNLLQSADLIVRSALKRHESRG
                     LHYTLDYPQMLPEARDTVLMP"
BASE COUNT         1288 a         2619 c         2772 g         1314 t
ORIGIN      
        1 catatcgagc aggatggccc ggcgatcggc ggcaaccggc cgatcgatgt ggcgctggcg
       61 ggcgatctgg cggcgatcct gccgcagctg tcgggccggc tggcgcgggc ggcgggggcg
      121 gatctggccc gctggtctgc cgccgaggcg gcgcggcggg cagcgctggc gcaagggccg
      181 tcggccggcg ccgggcccct ccacccggca cggctggtgg tggaagccac ccgcgccctg
      241 ccgcaggagg ccattctggt gcgcgatggc ggcgctttcg tgctgtttca gctggcctat
      301 gggcagggcc acccgcgcga tgtgctgtgg agccagaacc tggcccatct gggcacgggc
      361 ctgccctatg cgattggtgc ggcgcaggcg acaggcgggc agcggccggt ggtgctgctc
      421 accagcgatt cggcgctgct gttccacatt gccgagctgg agacggccgc gcggctgggc
      481 ctgccgatcc tgtgcgtggt ggcggtggat catcagtggg ggctggaggc gggcatgtat
      541 cgccgcctcc acggccccgc cacgccagag cccggcgccc actggagccg ggatgtgcgt
      601 ttcgacagga ttgctcgggg gctggcctgc cccgccagcc aggtgacgca ggcgcaggaa
      661 attgcccccg ccatggccgc cgcgctggct gccggcgggc cgtatctgct gcatgtgccg
      721 atcgatgcgg ccgctaatgc tggcgagatc cctgcctttg cggaattcgc cagctggtat
      781 gcgcaggcct gaaggcccta ggcctcagtg gccggatttg gccccgccgt ccttcggcac
      841 ccgcgtccac ggcacgaact ggcccagccc caccgaaccg cgatagaagc cgccggcacg
      901 atcgcgcagc tgcatgatat cgaggtggca cagctggtcg agcgaggtct tggtgacgag
      961 gatatcgtcc gaggcgagct ggtcgggcgc atcggtgcgg ttcacataga gcacgctgcc
     1021 cgaatcatag acgatcgccg tcttgtcgat caccgtggaa ctgcccagcg agatgatgct
     1081 gacacaatcc accggggcgc cggcctggcg gccctgcagc atcttggcca gctgctgctc
     1141 gccggtcagc cgctcgcgcg cggtggcagg cgcagcggtg cagccgatgc cggccagcgc
     1201 cagcatggcc aggcaggagg caggaagttt acgcatgatc tggtcctttc ccgcacccga
     1261 aagcaacatc accctatagg cccatccggc tgaatggcaa atgagcaggc ccgctcagcc
     1321 ggtgccgccc accgtcagcc cttccaccag caaggtgggc tggccaacgc cggcaggcac
     1381 agactggccg cccttgccgc acacgcccac gccttcatcc agcgccagat catggccaat
     1441 gcctgtcacc cgcgtcagca ccgaggggcc atcgccgatc agcgtggcgc ccttgatggg
     1501 ggcgcccagc ttgccgtttt ccaccagata ggcctctgtg caggagaaca cgaacttgcc
     1561 gctggtgata tccacctgcc cgccgccgaa gctcttggcg aagatgccgt tcttcatgcg
     1621 cgagagcagc tcggccggat cgtcgttgcc ggcgcgcatg aaggtgttgg tcatgcgcgg
     1681 catgggggcg tgggcatagc tttcgcgccg gccgttgccg gtgggggcca cgcccatcag
     1741 ccgggcgttc agccggtcct ggatgtagcc cttgaggatg ccatcctcga tcagcacgtt
     1801 ttcctgcgtc ggcgtgcctt catcatccac gctgagcgag ccgcgccggc ccgccaggct
     1861 gccatcgtcc accaccgtca cgccgggggc ggccacccgc tcgccaatgc ggccggaaaa
     1921 ggcgctggtg cccttgcggt tgaaatcgcc ttccagcccg tggcccaccg cttcatgcag
     1981 cagcacgccg ggccagccgg gggccagcag cacctgcatc tcgccagcgg gcgcggccac
     2041 cgattcgagg ttggtgagcg cctgggccag cgctgtgtcg atcgcgcggt tccagctctc
     2101 gggctcgaac aggcggtcat acagccagcg cccgcccatg ccgaagctgc ccgtctcgcg
     2161 ccggccgttc tgttcggcca cgatcgacac gttgagccgc accagcggac gcacatcgag
     2221 caccggcccc gcgccgccga aattgtcggc ccgcaggatt tccaccacgc tccagctggc
     2281 cgacagcgag gccgacacct gcgccacgcg cggatcgcgg gcgcgggcag cggcatcgat
     2341 cgcctccagc agcgccacct ttttggcaaa gggcaccaga tcgagcggcg aggtatcggt
     2401 gtagaggtgg cggttggtgc gggcaggggg cgccgggcgg gtgccttgcg ccgggtccag
     2461 cagggtcagc gtttcgccgg cgcggcgaat cgcggcggcg gaaatctcgt tggcatgggc
     2521 aaagccggtc gtctcgcccg agacaccgcg caggccaaag ccggcctctc ggctgtagtc
     2581 ggcggttttc agccggccat catcgaagcc gaaagcctcg ctggcggtga attgcaggaa
     2641 cagctcgcca tcctcgcagg cagccagcgc gctgccgacc agcttctggg cttcgtcggg
     2701 ggccagctgg ccgggggtgt agagcagcga gcgggggtcg gtagccaagg tcatgccccg
     2761 gatataggtg cgctgtggtt tttgtcgagt ggcgcgcgcc ctcgctgcgg cgcgcgcgat
     2821 caacccttgc gctcaaagat caatgcgcgc tcaaagatca atgcgcgtga tgcgcatcca
     2881 gcacgaagcg cttgtcgcaa taggggcagt cgacaaagcc cttctcgtcg atttccagcc
     2941 agatgcgcgg atggcccagc gcctggggcg cataattgcc agtgctgcgg atctcgccgg
     3001 cgccatcgca ggcgacgcgg gtggaggtga cggtgacggt ttcgggctgc tgggtcatgg
     3061 cagctgccga tagcagcacc ggcggcgcat ttccagcctt gcgctggccc ggcaggcggc
     3121 ggtaaagcgg cccgtcgtcg atccgggccg ggttggccct tgcaggggcc gccgggccgg
     3181 gcagatggcc gggggcgcct gcgtcacttt tcgcctttct gcgcatttgc ccgcaatctt
     3241 gcgggcgggg cgaattgtaa ggtgcaggca gcctcgctat ggacgacaga acgcccttcc
     3301 tgctagcggt cggggcactg ccgatgatcg caaagcggtg tgcatgcgcc ggtttacgag
     3361 tttgaatgtc attttcgcgc aattttcgcg cgctcgaagg gcccatggtc atcgctaacc
     3421 gagagctgct catctgcgat cttacccaga gctatgctcc ccgcggcggc ggtggcgtca
     3481 gcacgtatct gagtgagaag acgcgtcacg tcctttccaa gaccagccat cgcctgctgc
     3541 agatcgtgcc gggcgagcgc gaccggatcg tggaaaaagg gcggcacatc tgggtggagg
     3601 tgggcgccga ccaggtgcgc ggcagcccga actatcgctt catcctgcgc accggcgccg
     3661 tgcgcgagat cctcgagcgc tatcggcccg acatcatcga atcgcaatgc ccctgggtgc
     3721 tgccgtggac ggcgatcaac tatcgccgcg cccatccgga aaccgcgctg gtggccggct
     3781 atcacaccga ttttcccaat gtgcatgtgc accgtgtcgg ctcgcagatc ttcggcgaga
     3841 cgctgggcgg catcctgcgg cgcatcagca tcggctatgc caccttcacc taccgcgagt
     3901 ttgaccgcgt ctatgcgatg aacgagcaga tgcgcgagca gctggccggc tatggcatcc
     3961 gccatgtcga cgtgctgacg ctgggggtgg ataccgacca gttcaacccc gctcgccgcg
     4021 atcccaccta tcgtgcccgc atggggctgg ccggccatgg cccactgctg gcctatgccg
     4081 ggcggctgga caatgaaaag cgcgccgacc ggctggtgga aatgctgcgc gagctgccgc
     4141 gcgacatggg cgcggcgctg gtgatgtttg gcgatggcaa gctgcgcgag aagatcatgt
     4201 ccatcgccca ggcggaaaac ctgccggtgg cgctgcccgg ctttgtcgat gaccgtgatg
     4261 cgctggccgt ggcgctggcc tcgtccgaca tctatgtgtc ggcgatggcg gatgaaacct
     4321 tcggcatctc ggtgatcgag gcgcaggcct cgggcctgcc ggtggtgggc gtggcctcgg
     4381 gcgcgatgcc cgcgcgcgtg ccgcaggggc tgggcctgct gggcccggtg gacgatgtgg
     4441 tgacgatggc ggccaatgtg cagcagatct gggcgaatga tccgcgcgcg atcggcgcgg
     4501 ccgcccgggc ccatgccgcg cgccgctaca gctgggagaa gaccttctcc tacctcatcg
     4561 gcgaggtcta tcccacggcg ctggaaagcg ccgcgcggcg ggtgcattcg ccgcgcgcct
     4621 ggaagatttc ggcgcgggcg gccagcccct tgcggggcgc cgcctgatcc gggcgggcgg
     4681 ggctttaccg ctttaccgtt ccggcaaaac aggccaatag cccgcgcatg agcgcgtcct
     4741 cctccctgcc gcccgccatt tccatccgca atctggtcaa gcgctatgcc ggctcggccg
     4801 gggcgccggg caagctggcg ctgggcgggg tcagcttcga tgtgccgcag gggcagatct
     4861 tcggcctgct gggccccaat ggcgcgggca aatccacgct gatcaacacg ctggcgggcc
     4921 tggtcatgaa gaccagcggc gaggtggata tctggggttt cgatatcgat cgcgaccggc
     4981 gcaatgccag ccgcgccatc ggcatcgtgc cgcaggagat cgtcttcgat cccttcttca
     5041 cccccttcga ggtgctggag atccaggccg gcctctatgg cgtgcccagg gcgcagcgcc
     5101 gcacggatga gttgctcgcc gccgtgcacc tcagcgacaa ggcgcgggcc tatgcgcgca
     5161 cgctgtcggg cggcatgaag cggcgcctgc tggtggccaa ggcgatggtg caccagccgc
     5221 cggtgctggt gctcgacgag ccgacggcgg gggtggatgt ggagctgcgc cgccagctgt
     5281 gggagctggt gaaagggctg aacgacgagg gcgtgacggt ggtgctgacc actcactacc
     5341 tggaagaggc cgaggagctg tgcgaccgca tcgccatcat caaccacggc cagctgatcg
     5401 ccaacaagcc cacgcgcgaa ctggtgggca tggcccgcga gaaggtggtg gtgctgacgg
     5461 tggaccgcga ggtgagcgcg ccgcctgccc atccggcctt catcaaggcg caggtaaccg
     5521 ccccccgcac gatcgagatc agctacgaca aggaccgcgc cacggccggc gagatcctcg
     5581 ggctggtgca ggcgcagggg ctgggcatcg tcgatgtcag cacgcgtgag gcggatctcg
     5641 aggatgtctt cgtggccctg accagcgagc ggccgcaggc cggctgaaga gccgcgtgcc
     5701 cgggcccgca tgagggcccg gggcacagcg cctggcgttc agctgccctt gtggccgttt
     5761 tcgcgctgat cctcgatcag ccggccggcc ttgccggtgt cggtcagcca atccttttcc
     5821 cagcggccct tgtcacgccg gcgaatcggc ttgccgcaaa agcggcactt gccgatgaaa
     5881 tgcagcccgt cccacttcac ccgattgcga tcgggggcgt ggcggttgaa caggcacaga
     5941 ggcaagcgca gggccatggg gagaagggct ccgggcagga aaatatggtt gacagcaaaa
     6001 tcgggtggcg gcgtctgagc gaaaaacgct tccggtaaat tgcccggaaa ccgggtaact
     6061 ctttggtcac gctttcgtta acctctcata atttaacgaa aggttagtcg gcaagcgcca
     6121 aggcagagtg aaatgtaaaa aatgtggaag tgggggaatc tgccacaggg gggctgtcat
     6181 tgctttgcaa ttttggcccg agaggcctag caggccccca tgcgtttcct gaccccccct
     6241 gtcgcgagtc cgctccctga tatctgccag acgaggaagg gggcgcgccg atgagcgggg
     6301 cggcaccctc ggccgatgtc atcgtggtgg gctcgggcgc ggcagggtta acggcggcgc
     6361 tggccctggc cgagcggctg aaggtgctgg tggtggccaa gggcagcctc accggcggct
     6421 ccaccgcctg ggcgcaaggg ggcattgccg ccgtgctgga tgccggcgac actttcgagg
     6481 atcatatccg cgacacgatg gtggccggcg ccggcctcaa ccgcatcgag acggtggagc
     6541 atgtggtgga acgcgccccc catgcgatcg accggctgat cgatctgggc gtgccgttca
     6601 acaccgaagg caacaacctg catctgacgc gggagggcgg ccattcccac cggcgcatcg
     6661 tgcatgtgga tgatgccacc ggctgggcgg tgcaatcggc gctgctgaag gcggccgagg
     6721 cgcaccccaa catcaccctg ctgccgggcc gggcctgcat cgatctcatc accggccggc
     6781 atgagctgcg ctattcgggc tctggccggg tgtggggcgt ttatgcgctg gatgaggcca
     6841 gcggccgggt ggaagcccac accgcccgcg caaccatcct ggcaaccggc ggggcggggc
     6901 gctgctatct ctattccaca gcgccgcgcg gggccaccgg cgatggcatc gccatggcct
     6961 ggcgggccgg cgcgcgcgtg gccaacatgg agatgatgca gttccacccg acctgcctct
     7021 acaacctcga cgtcaagaac ttcctcatca ccgaggccgt gcgcggcgag ggcgggcggc
     7081 tgatcaaccc gcgcaccgcc aagcgcttca tgcccttcta cgacgagcgg ctggaactgg
     7141 ccccgcgcga tgtggtggcc cgcgcgatcg acagcgagat caagaagttc ggtctcgatt
     7201 atgtccatct cgacatcagc cacatgccgt ccgatttcgt tgccgagcat ttccccaaca
     7261 tccgcgagaa gctggcgggg ctggggatcg acatgacgaa gcagccgatc ccggtggtgc
     7321 cggcccagca ctacacctgc ggcggcatcg ttaccgattt gcacggccgc accgatctgc
     7381 ccggccttta tgccgcgggc gaatgcagcg aaagcggcct gcatggcgcc aatcgcctgg
     7441 cctcgaacag cctgctggaa tgtttcgtct ttggcgaggc ggccgcggcc gatatcctgg
     7501 ccaactgggc cgatttcgcc cccccgccgc aggtgcgcgc atgggacgaa agccgcgcca
     7561 ccgattccga cgaggaagtg atcatcaagc agaactggac ggaaatccgc cggttcatgt
     7621 ggaactatgt cggcatcgtg cgcactacca agcggctgga gcgggcccag caccgcatca
     7681 acatgctgtt tggcgaggtg gaggaatatt acagcgcctt ccgcgtgacg accgacctga
     7741 tcgaactgcg caacctgctg caaagcgccg atctcatcgt gcgcagcgcg ctgaagcggc
     7801 atgaaagccg gggcctgcat tacacgctgg attacccgca gatgctgccc gaagcgcgcg
     7861 atacggtgct gatgccttaa gggcaaagcg gatggcaccg ccggaggctt tgaggattgc
     7921 ggctttgccg ccgggcgccc tgccggaaag ccggcaccac cctctacagt aacgggagcg
     7981 cgagggtgtt aac
//
LOCUS       DASRYE010000153        10291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_95811,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000153 DASRYE010000000
VERSION     DASRYE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95811"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFF98_08220"
     CDS             complement(<1..685)
                     /locus_tag="VFF98_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63654.1"
                     /translation="MSDIARPTAAPALDEALSKVPAVTLGFWIIKILATTLGETGADL
                     FTQQYEMGYAAGIALFATPLVLLVAAQIRARAFHPLLYWATILASTTAGTALADFFDR
                     SLGIGYLGGSLTLVACVGLSLLAWRLVLGTVSVASVATPRAEIFYWITITFSQTLGTA
                     LGDWTADSFGDATFMGLESGYVLGALIFAGLLGAIALLYRFTAASRTGLFWAAFILTR
                     PLGATVGDFL"
     gene            complement(736..1764)
                     /locus_tag="VFF98_08225"
     CDS             complement(736..1764)
                     /locus_tag="VFF98_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZU63655.1"
                     /translation="MNRTCFARHAGAAVAVALLAAAGPVLADAPAYKVEARIAGPDGG
                     WDFAQIDAAGGKLYVARSNAVMAVDLASRQAATLAPANGAHQVLPIDGGKTIAETDGK
                     TDLTRFIDAASGQVLAEVPSGKKPDAAFYDAATNRVVVMSPGSSTVTQIDAASRKVVN
                     SFALAGGLEYGAAAGNGRAFINLEEANALAEVDLKAGKLLRKIALPGCDGPTGLALVA
                     GGTRAISACANGVAMVVNTASGKVVGKLAIGRDPDAVVADEARGLAFIPCGGSGTLVA
                     ISIADKAHIAVAGTIATQVGAKTAALDPRDGRIYLPTATLGQPAPGAKRGKPGPGTFV
                     VLVVAPQR"
     gene            1997..3040
                     /locus_tag="VFF98_08230"
     CDS             1997..3040
                     /locus_tag="VFF98_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU63656.1"
                     /translation="MEYRKLGRTGISVSAVCLGTMTWGSQNSEAQAHEQIDYALSRGI
                     TFLDTAEMYPVTPSRVETLGRTEEYIGSWIAQSGRRGEIVLASKVAGPSRLLPIRGGD
                     NRLDAKNINAAIDASLKRLKTDYLDLYQLHWPDRGVPMFGGRGLATLADAPDTVPLEE
                     TLEAMAALVSAGKVRAIGVSNETPWGVAEALRLAGAGTAPRIASIQNAYNLLNRVFEG
                     GLSEFALREDVGLLAYSPLAAGYLSGKYLGGVVPAGSRGDVARQFTRYFTPSQANATA
                     RYVGIAHAFGLSPEALALGFVYSRPFVTSSIIGATSLDQLKADIDGSLAVLPPEALEA
                     IESVQATWPDPCP"
     gene            3144..4832
                     /locus_tag="VFF98_08235"
     CDS             3144..4832
                     /locus_tag="VFF98_08235"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZU63657.1"
                     /translation="MSAGHPAPAPNRPARALFATLLSDGAWGRNCLLWGSLALTVAAP
                     LLALRHHRALWLLALALPVLAVCLVDLFQKGHSLRRNFPAAARFRWMFEWLRPYLRSY
                     IVESDLDGRPFSHEERALVYARAHGTSDAHPFGTELDVYSGEYEWLAHSMRPNPDAPA
                     DMRVKVGSDQCAKPYMASRLNISAMSFGSLGSHAVEALNLGAKMAGCYHDTGEGAVSP
                     YHLKHGGDVVFQVASGYFGCRDGQGRFDPARFADTAQAESIRMIEIKLSQGAKPGHGG
                     MLPAAKITPEIAATRGIPMGEDCISPPAHSAFSSPRELLEFAAQLRELSGGKPVGLKL
                     CVGFPHEIFALAKAMLASGITLDFIVVDGAEGGTGAAPTELSDRVGMPLREGLMVVRN
                     ALVGTGLKTQVRLAASGKVTSGASIAMNAALGADWSNAARAFMFSLGCVQSLKCHTGT
                     CPTGIATQSPARQRGLVVPEKAERVARFHKATIHALHEIVVAAGLDNPGDFRPSHLRH
                     RINVAEMRQMDEIYPFARPGELLGGGGHPTLRRWWDAASPDSFRRVDDDRLALT"
     gene            complement(4843..5265)
                     /locus_tag="VFF98_08240"
     CDS             complement(4843..5265)
                     /locus_tag="VFF98_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU63658.1"
                     /translation="MPESQAMLRKLIERLRGRRGGQAGRENRRDARHKVNQVVVASHP
                     RVGLTSLLLVDISAGGAQCEAAAGLRRGDRVMVDLPLEGERAAEVMWSLAGRAGLRFA
                     EPLAPDALRALITIMRFGPTCDMCAGEDDPCEDHRFPG"
     gene            5444..6274
                     /locus_tag="VFF98_08245"
     CDS             5444..6274
                     /locus_tag="VFF98_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZU63659.1"
                     /translation="MTQSVQSPTNEAVSPVRGGYMDFQQKLDAMRIRCAADVRPAALA
                     FRDFTHELFDCRIAACHNIAVKSQMCDDAGTILATDVFGWTDEDKWWRTPHLALASPL
                     TTACRFEAEPFWVNAGGFRTRDRNSWVEQIDISDFEKRAFTRAAIVVPVHLPFGHIGV
                     VSFSPTDSSILDLSHEYEAYSDLLGMFTRTFISSYQRIMVKPERIPAGGSLLSKREVE
                     CLRWAAIGKTDHEIAMIMSRSRATVRFHIQNAAVKLDAVNRSQTVFKAAQLGYIGLNS
                     "
     gene            complement(6371..7579)
                     /locus_tag="VFF98_08250"
     CDS             complement(6371..7579)
                     /locus_tag="VFF98_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63660.1"
                     /translation="MHIELSAQDLAFQAEVRAFLAAELGPDLRAGARATPTVFAEPDI
                     SQRWQQILHRKGWLGYNWPVQFGGTGWTPTQRYIFEKECALADAPDLPVLSLKLLAPV
                     LHAFGSPAQQAELLPRILSGADYWCQGFSEPSSGSDLANLKTRAVREGDHYVINGGKL
                     WTTHAQFANKIFCLVRTNPDVKPQAGLSFLLVDLATPGIVVQPIIGLAGDHEVNAVFF
                     DDVKVPAANLVGEEGQGWTIAKFLLEHERGGSCHAPRLLADIALLRASAARESDGLGG
                     SLLDDAVFAQTLAATEFDALALEMTELRILAELAQGRKPGPQTSLVKLAASNIRQQVD
                     GLAMRVFGYAGLQLTRRRPLYAADADVAICCKEAQVAPARYLNSRAWTIFGGSNEIQR
                     TIIAKTVLGL"
     gene            7821..8906
                     /locus_tag="VFF98_08255"
     CDS             7821..8906
                     /locus_tag="VFF98_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU63661.1"
                     /translation="MDFSPTEEQQMLRDLIERFAADTYPAADRPHYRVPATGFAPGAW
                     SQLAELGLLAMAFPEELGGLGAGPVELMILGEALGKGVVPEPVLAEVLQAGLLLARAG
                     TEEQKDRWLEGVLSGSAHLALAFAEPDSRFELATPATSCADGRLSGTKTCVMGGSACD
                     AVIVSARGPDGAVGLWLAELSAPGIARTDYRLLDGQPAMELTFEGTPATPLAEGLAAL
                     ETLVVDLRVAIVAEMVGLMQTMFDLTVDYVKVRKQFGAAIGSFQAIQHRLADQYMMLE
                     QSRSLLLRAALATPAEREAAGLAAKAFVARAGVALGEEAIQLHGGMGVTDELLVGHAH
                     KRLLLLASLFGDADREAARYMALTRAA"
     gene            complement(8938..9936)
                     /locus_tag="VFF98_08260"
     CDS             complement(8938..9936)
                     /locus_tag="VFF98_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU63662.1"
                     /translation="MHNRQWLIARRPIGRALEAEDFLWAEGTAPAPGPGEVLVRVDWL
                     SFDPAQKGWMENVADYVAPSAIGDPVPAFACGEVVASADPAFAPGDKVTGRLSWQDYA
                     CVPAAALSRLPDDGHGSRNLGVLGITGMTAFFGLHRVGRPVAGDTMVVTGAAGATGSL
                     VGQLGKIAGCRVIGLAGGADKCRWLTGEMGFDAAVDYRAPDARQQVKALVPQGIDVLW
                     DNVGGPLLDTLLPRIATGARVVICGAISTYSSATRGAGPANYMQLVFRRARMEGFIAL
                     DYAAEYPMARARLAQWLAEGRITAQEDVAQGLEQAPQTLMRLFAGANRGKQVLQMR"
     gene            10118..>10291
                     /locus_tag="VFF98_08265"
     CDS             10118..>10291
                     /locus_tag="VFF98_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU63663.1"
                     /translation="MDISERALELGARVEAFVRSEVIAYESDPRRDHHGAPTDELVME
                     LREKARAAGVLTPH"
BASE COUNT         1692 a         3555 c         3426 g         1618 t
ORIGIN      
        1 cgaggaaatc gcccaccgtg gcgcccagcg gccgggtgag gatgaacgcc gcccagaaca
       61 gcccggtgcg cgaggccgcc gtgaagcgat agagcagcgc gatggccccc agcaggccgg
      121 caaagatcag cgcgcccaac acatagcccg attccagccc catgaaggtg gcatcgccaa
      181 agctgtcggc cgtccagtcg cccagcgcgg tgcccagcgt ttgcgagaag gtgatggtga
      241 tccagtagaa gatctcggcc cggggcgtgg caacgctggc caccgacacc gtgcccagca
      301 ccagccgcca ggccagcagc gagaggccca cgcaggccac cagcgtcagc gatccgccca
      361 gatagccgat gcccagcgag cggtcgaaga aatcggccag cgccgtgccc gccgtggtgg
      421 aagccaggat agtggcccaa tagagcagcg gatggaaggc gcgggcgcgg atctgggccg
      481 ccaccagcag caccagcggc gttgcgaaca gggcgatgcc ggcggcatag cccatctcat
      541 attgctgggt gaacaggtcg gcgcccgttt cacccagcgt ggtggccagg atcttgatga
      601 tccagaaacc cagcgtcacc gccggcacct tggaaagggc ctcatccagg gcgggggcgg
      661 cggtggggcg tgctatgtcg ctcatcggca gcgaaccttt cttgcaatcc tgcaaaaacg
      721 caaggctagc cgcgcttagc gctgcggcgc caccaccagc acgacgaaag tgcccgggcc
      781 aggcttgccg cgcttggcgc cgggggcggg ctggcccagc gtggcggtgg gcagatagat
      841 gcgcccgtca cgcgggtcga gcgcggcggt cttggcgccc acctgcgtgg caatggtgcc
      901 ggcaacggcg atatgcgcct tatcggcaat cgagatggcc accagcgtgc cgctgccgcc
      961 gcaggggatg aaggccaggc cccgggcctc gtcggccacc acggcatcgg ggtcgcggcc
     1021 gatcgccagc ttgcccacca ccttgccgct ggcggtgttc accaccatcg ccacgccatt
     1081 ggcgcaggcc gaaatggcgc gcgtgccgcc ggccaccagc gccaggcccg tggggccatc
     1141 gcagccgggc agggcgatct tgcgcagcag cttgcccgcc ttcagatcca cctcggccag
     1201 cgcattggct tcttccagat tgatgaaggc gcggccattg ccggcagcgg cgccatattc
     1261 cagcccgccg gccagggcga agctgttcac caccttgcgg ctggcggcat cgatctgggt
     1321 aaccgtgctg ctgcccgggc tcatcaccac cacgcgattg gtggcggcat catagaaggc
     1381 ggcatcgggc ttcttgccgc tgggcacttc ggccagcacc tggccggagg cggcatcgat
     1441 gaagcgggtg agatcggtct tgccgtcggt ttcggcgatg gtcttgccgc cgtcgatcgg
     1501 cagcacctgg tgggcgccat tggccggcgc cagcgtggcc gcctgccggc tggccagatc
     1561 caccgccatc accgcattgg agcgcgccac ataaagcttg ccgccggccg catcgatctg
     1621 ggcgaaatcc cagccgccat cggggccggc aatgcgcgct tccaccttat aggcgggcgc
     1681 atcggccaga acggggccgg cagcggccag cagggcaacg gcgacagcag cgccggcatg
     1741 gcgcgcaaaa caggtgcggt tcattcgaat tgtctcccgg ccacgggccc gggccggcgc
     1801 cccccgccac gccccccttg cggccatggc gcgagaggtg gccgccattt gcgtcaatcc
     1861 cgtgacaagg aagccgggca aaagattggt ttttcgcaat gtgcccggcg cccgccgcac
     1921 ccctgcgcgg aaccattacc gcagcgcagc gttgccccgc ttgacagcgc ccacccccgg
     1981 gccgaggaat aagcgcatgg aatatcgcaa gcttggcaga accggcatca gcgtcagcgc
     2041 tgtgtgcctt ggcacgatga cctggggatc gcagaacagc gaggcgcaag cccatgaaca
     2101 gatcgactat gcgctgagcc gcggcatcac cttcctcgat actgccgaga tgtatcctgt
     2161 cacccccagc cgggtggaaa cgctgggccg caccgaggaa tatatcggca gctggatcgc
     2221 ccaaagcggc aggcgcggcg agatcgtgct ggccagcaag gtggccggcc cctcgcgcct
     2281 gctgcccatc cgcgggggcg acaaccggct ggatgccaag aacatcaacg ctgccatcga
     2341 tgccagcctg aagcgcctga agaccgacta cctcgacctt taccagctgc actggcccga
     2401 tcgcggcgtg ccgatgtttg gcgggcgcgg ccttgccacg ctggccgatg cgcccgacac
     2461 tgtgccgctg gaagaaacgc tggaggcgat ggccgcgctg gtgagcgccg gcaaggtgcg
     2521 cgccatcggc gtgtcgaacg agacgccctg gggcgtggcc gaggcgctgc gcctggccgg
     2581 cgcgggcacc gccccgcgca tcgcctcgat ccagaacgcc tacaacctgc tcaaccgcgt
     2641 gttcgaaggg gggctgtcgg aatttgccct gcgcgaggat gtcggcctgc tggcctattc
     2701 gccgctggcg gcgggctatc tgtcgggcaa atatctgggc ggggtggtgc cggccggatc
     2761 gcgcggcgat gtcgcccggc agttcacccg ctatttcacc ccgtcgcagg ccaatgccac
     2821 ggcgcgctat gtgggcattg cccatgcctt tggcctcagc cccgaagcgc tggcgctggg
     2881 ctttgtctac agccggccgt ttgtcaccag ctcgatcatc ggcgccacct cgctcgatca
     2941 gctgaaggcc gatatcgatg gcagcctggc cgtgctgccg ccagaagcgc tggaggccat
     3001 cgaaagcgtg caggccacct ggccagaccc ctgcccctga ccgccacccc ggcccgcagg
     3061 gagagggctg gcctgtccat ctcctgcaac gcgccggggc tagagccgat tcgctgccgc
     3121 aagcaggccg caaggagagg atgatgagcg caggacaccc cgccccggcc cccaaccggc
     3181 ccgcccgcgc cctgttcgcc acgctgctca gcgatggcgc atggggccgc aattgcctgc
     3241 tgtggggcag cctggcgtta acggtggccg ccccgctgct ggccctgcgc caccaccgcg
     3301 cgctgtggct gctggcgctg gcgctgccgg tgctggcggt gtgcctggtc gatctgttcc
     3361 agaagggcca ttcgctgcgc cgcaattttc ctgccgcggc gcgctttcgc tggatgttcg
     3421 aatggctgcg gccctatctg cgcagctata tcgtggaaag cgatctggat ggccgcccct
     3481 tcagccatga ggaacgcgcg ctggtctatg cccgcgccca tggcaccagc gatgcccacc
     3541 ccttcggcac cgagcttgat gtctattccg gcgaatatga gtggctggcc cattccatgc
     3601 gccccaaccc cgatgccccg gccgacatgc gggtgaaggt cggctctgat caatgcgcca
     3661 agccctatat ggcctcgcgg ctgaacattt cggcgatgag cttcggctcg ctgggatcgc
     3721 atgcggtgga agcgctgaac ctgggcgcca aaatggccgg ctgctatcac gataccggcg
     3781 agggcgccgt ctcgccctat cacctgaagc atggcggcga tgtggtgttc caggtggcct
     3841 cgggctattt cggctgccgc gatggccagg gccgcttcga tccggcgcgc tttgccgata
     3901 ccgcccaggc cgaatcgatc cgcatgatcg agatcaagct gagccagggc gccaagcccg
     3961 gccatggcgg catgctgccc gccgccaaga tcacgccgga aatcgcggcc acccgcggca
     4021 tccccatggg cgaggattgc atcagcccgc cggcccattc ggccttttct tccccacgcg
     4081 aattgctgga atttgccgcc caattgcgcg aactgtcggg cggcaagccg gtggggctga
     4141 agctgtgcgt gggcttcccg cacgagatct tcgcgcttgc caaggccatg ctggccagcg
     4201 gcatcacgct ggatttcatc gtagtggatg gcgccgaggg cggcactggc gctgccccca
     4261 ccgaactgtc ggaccgggtg ggcatgccgc tgcgcgaggg gctgatggtg gtccgcaacg
     4321 cgctggtggg caccggcctc aagacgcagg tgcgcctggc tgccagcggc aaggtcacct
     4381 cgggcgcctc catcgccatg aacgcggccc tgggcgccga ctggagcaat gccgcgcgcg
     4441 ccttcatgtt ctcgctgggc tgcgtgcaat cgctcaagtg ccacaccggc acctgcccca
     4501 ccggcatcgc cacccaatca cccgctcgcc agcgcgggct ggtggtgccc gaaaaggccg
     4561 agcgcgtggc ccgcttccac aaggcaacga tccatgcgct gcacgagatc gtggtggcgg
     4621 ccgggcttga taaccccggc gatttccgcc ccagccacct gcgccaccgc atcaacgtgg
     4681 ccgaaatgcg ccagatggac gagatctatc cctttgcgcg gccgggcgaa ttgctgggcg
     4741 gcggcggcca ccccacgctg cgccgctggt gggatgcggc cagccccgac agtttccgcc
     4801 gggtggacga cgaccgcctg gcgctgacct gagccggccg cctcagccgg ggaaccggtg
     4861 gtcctcgcaa gggtcgtctt cgccggcgca catgtcgcag gtggggccaa agcgcatgat
     4921 ggtgatcagc gcgcgcagcg catcgggcgc cagcggctcg gcaaaacgca ggcccgcgcg
     4981 gccggccagg ctccacatca cctcggccgc ccgctcgccc tccagcggca ggtcaaccat
     5041 cacccggtcg ccgcgccgca gcccggcggc cgcctcgcat tgcgcgccgc cggccgaaat
     5101 atccaccagc agcaggctgg tcagccccac ccgcggatgg ctggccacca ccacctgatt
     5161 gaccttgtgg cgcgcatcgc gccggttttc gcggccggcc tgcccgccac gccggccgcg
     5221 caggcgttcg atcagtttgc gcagcatcgc ttggctctcc ggcatcgctt tatccgtgct
     5281 tgtccggtat acaaccttta caaataccta acacctgcga aagccgccgc cggcccggca
     5341 ttttggccat ttggccacag tgctgcggca aaatcactcg aaaacgggat gaatccgata
     5401 atttgcggca gagcggactt gatttaaccg cgcgactaag gctatgaccc aaagcgttca
     5461 atcgcccacc aacgaggcgg tttctccggt gagaggaggc tatatggact tccagcaaaa
     5521 gcttgatgca atgcgcattc gctgcgcagc agatgtacgc ccggcggccc tggccttccg
     5581 tgatttcacg catgaactgt tcgattgccg cattgccgcc tgccacaaca tcgcggtgaa
     5641 aagccagatg tgcgatgacg ccggcaccat ccttgccacc gatgtgttcg gctggacgga
     5701 tgaggacaaa tggtggcgca cgccgcatct ggcgctggcc tcgccgctga cgacggcctg
     5761 ccgcttcgag gcggagccct tctgggtgaa tgccggcggc tttcgcacgc gcgatcgcaa
     5821 cagctgggtc gagcagatcg acatctcgga cttcgaaaag cgcgccttca cccgcgccgc
     5881 catcgtcgtg ccggtgcacc tgccctttgg ccatatcggc gttgtcagct tcagcccgac
     5941 ggacagttcg atcctcgacc tgtcgcacga atatgaagcc tattccgact tgctgggcat
     6001 gttcacccgc accttcatct cgtcctatca gcggatcatg gtgaagccgg agcgcatccc
     6061 ggccggcggc tcgctgctgt cgaagcgcga ggtggaatgc ctgcgctggg cggccatcgg
     6121 caagaccgac cacgagatcg ccatgatcat gtcgcgcagc cgcgccaccg tgcgcttcca
     6181 catccagaac gcagccgtga agctggatgc ggtgaaccgc agccagaccg tgttcaaggc
     6241 cgcgcagctg ggctatatcg gcctcaattc gtaaatccgc gaaaggggtc ccagggcccc
     6301 gccaccagtt gaacagccga accggcgccg ctttcaaacg gcgccggttt gctgttgggg
     6361 caggcctaga tcacaggccc agcacggttt tggcgatgat cgtgcgctgg atctcgttgc
     6421 tgccgccaaa gatcgtccag gcgcggctgt tgagatagcg ggccggcgcc acctgcgctt
     6481 ccttgcagca gatcgccaca tcggcatccg cggcatagag cgggcggcgg cgggtgagct
     6541 gcagcccggc atagccgaac acgcgcatcg ccagcccatc cacctgctgg cggatgttcg
     6601 aggcggccag cttcaccagc gaggtctgcg ggccgggctt gcggccctgg gccagctccg
     6661 ccagaatgcg cagctcggtc atttccagcg ccagcgcgtc gaactcggtg gccgccagcg
     6721 tctgtgcaaa caccgcatcg tcgagcaggc tgccgcccag cccgtcgctc tcgcgcgccg
     6781 cgcttgcgcg cagcagggca atatcggcca gcaggcgcgg cgcgtggcac gagccgccgc
     6841 gctcgtgttc gagcaggaac ttggcgatcg tccagccctg cccctcctcg cccaccagat
     6901 tggccgcggg caccttcaca tcgtcgaaga acaccgcatt cacttcatga tcgccggcaa
     6961 ggccgatgat cggctgcacc acgatgcccg gcgttgccag atccaccagc aggaaggaga
     7021 ggcccgcctg cggcttcaca tcggggttgg tgcgcaccag gcagaagatc ttgttggcga
     7081 actgggcgtg ggtggtccac agcttgccgc cgttgatcac ataatggtcg ccctcgcgca
     7141 cggcgcgggt cttgagattg gcaaggtcgg aaccggagga aggctcggaa aagccctggc
     7201 accagtaatc cgcgccggaa aggatgcgcg gcagcagctc ggcctgctgc gccgggctgc
     7261 caaaggcatg cagcacaggc gccagcagct tcaggctcag caccggcaga tccggcgcat
     7321 cagccagcgc gcattccttc tcgaagatgt agcgctgggt gggcgtccag ccggtgccgc
     7381 cgaactgcac cggccagtta tagcccagcc agcccttgcg atggaggatc tgctgccagc
     7441 gctggctgat gtcgggttcg gcaaagacgg tgggcgtggc gcgggcgccg gcgcgcagat
     7501 ccgggcccag ttcggccgcg agaaaggcgc gtacctcggc ctggaatgcc agatcctgtg
     7561 cgctcagctc gatatgcatt gcccggtccc ttccctcctt gtgccgccgg ccgtccccgg
     7621 ctttggcttg gcggcttact cctgcctgct atgtgccagc cgcccggccg gtgcctctgg
     7681 cgaaaatgct aggaaccggg gcgaatccgt cccttcaggc agctagcgca aagaggaggc
     7741 gcgagggggg cagcgcccat ttatactgcg cccaagacag caaaaccgct gcgccataac
     7801 gccagacgga gtggatctac ttggacttct cgcccaccga agagcagcag atgctgcgcg
     7861 atctgatcga acgcttcgcg gccgatacct atcctgccgc cgatcgtccg cattatcgcg
     7921 tgccggccac cggctttgcc cccggcgcct ggagccagct ggccgaactg ggcctgctgg
     7981 ccatggcctt ccccgaggaa ctgggcgggc tgggcgcggg gccggtggag ctgatgatcc
     8041 tgggcgaggc gctgggcaag ggcgtggtgc ccgaaccggt gctggccgaa gtgttgcagg
     8101 ccggcctgct gctggcccgc gccggcaccg aggaacagaa ggaccgctgg ctggaagggg
     8161 tgctctctgg cagcgcccat ctcgccctcg cctttgccga gcccgacagc cgctttgaac
     8221 tggcaacgcc ggccacttcc tgcgccgatg gccgcctttc cggcaccaag acctgcgtga
     8281 tgggcggcag cgcctgcgat gcggtgatcg tcagcgcgcg ggggccggat ggcgccgtgg
     8341 gcctgtggct ggccgagctt tccgcccccg gcatcgcccg caccgattac cgcctgctgg
     8401 atggccagcc cgccatggag ctgaccttcg agggcacgcc ggccacgccg ctggccgagg
     8461 ggctggcagc gctggaaacg ctggtggtgg acctgcgcgt ggcgatcgtc gccgaaatgg
     8521 tcggcctgat gcagaccatg ttcgacctga cggtggacta tgtgaaggtg cgcaagcagt
     8581 ttggcgcggc catcggcagc ttccaggcca ttcagcaccg cctggccgac cagtacatga
     8641 tgctggagca atcgcgctcg ctgctgctgc gcgccgccct tgccaccccg gccgagcgcg
     8701 aggccgccgg ccttgccgcc aaggccttcg tggcgcgcgc cggcgtggcg ctgggcgagg
     8761 aagcgatcca gctgcatggc ggcatgggcg tgaccgacga gctgctggtg ggccatgccc
     8821 acaagcgcct gctgctgctg gccagcctgt tcggcgatgc cgatcgggaa gcggcccgct
     8881 acatggcgct gacgcgggcg gcctgagggg gcaggagggc gcgggcgccc tcccccttca
     8941 ccgcatctgc aacacctgct tgccccggtt ggcgccggca aacaggcgca tcagcgtttg
     9001 cggcgcctgt tccaaccctt gcgccacatc ctcctgcgcg gtgatgcgcc cttctgccag
     9061 ccattgcgcc aggcgggcgc gggccatcgg atattcggcg gcataatcca gcgcgataaa
     9121 gccttccatg cgcgcccggc ggaacaccag ctgcatgtaa ttggcgggcc ccgccccgcg
     9181 cgtggccgag gaataggtgg agatcgcccc gcagatcacc acccgcgcgc cggtggcaat
     9241 gcgcggcagc agcgtgtcga gcagcggccc gccgacattg tcccacagca catcgatgcc
     9301 ctggggcacc agcgccttca cttgctggcg ggcatccggc gcgcggtaat cgaccgcggc
     9361 atcaaagccc atctccccgg tcagccagcg gcatttgtcg gcgccgccgg caaggccgat
     9421 cacccggcaa ccggcaatct tgcccagctg gcccaccagc gatccggtgg cgccggcagc
     9481 gcctgtcacc accatcgtgt cgcccgccac cggccggccg acgcggtgga ggccgaagaa
     9541 ggcggtcatg ccggtgatgc caagcacgcc aaggttgcgc gagccatgcc cgtcatccgg
     9601 cagcctgctg agcgcggcgg ccggcacgca ggcataatcc tgccaggaaa gccgccctgt
     9661 caccttgtcg cctggggcaa aggcagggtc ggcgctggcc accacctcgc cgcaggcaaa
     9721 ggcgggcacg ggatcgccaa tcgcgctggg cgccacataa tcggcgacat tttccatcca
     9781 ccccttctgc gccggatcaa agctcagcca gtccacccgc accagcacct cgccggggcc
     9841 cggggctggc gctgtgcctt cggcccagag gaaatcctct gcctccagcg cccggccgat
     9901 cgggcggcgg gcgatcagcc actggcggtt atgcatctgt gttctccttc gggcgggcgg
     9961 atttggccgg ttgcgaggcc agttacgggg cctatggctg gcatggtgcg cacgcaggcg
    10021 gccaacaact aaccatgagg ccagttgccg ctggccgggc caaatgcttg gcaggaaggc
    10081 agacaaaccg gatcaggcca gaggacttgc acaggcgatg gacatcagcg aacgcgcgtt
    10141 ggaactggga gcacgggtag aagccttcgt ccgctccgag gttattgcct atgagagcga
    10201 tccgcgtcgg gatcatcatg gtgcgccgac ggatgagctg gtgatggagc tgcgggagaa
    10261 ggcgcgggcg gcgggtgtgc tgacaccgca t
//
LOCUS       DASRYE010000154         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_220195,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000154 DASRYE010000000
VERSION     DASRYE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220195"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1369)
                     /locus_tag="VFF98_08270"
     CDS             complement(110..1369)
                     /locus_tag="VFF98_08270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU63664.1"
                     /translation="MPAHASALPHMPAPALAAAQASGWQCAAITDPQALAALAPEWNA
                     LWARADAPRLSQGAEWALAGWRICAAPRGARLWVLTLREEGRLVLVWPLEVRREKGIR
                     IARPLGSDGTEYDGLLHAASPRAGAWLAAAWAYAAAHLPADGVVVPFVGEGSARAASL
                     VGKGLWQRRETLPAPFVRLRDGAGWQAYWQSRSKNLRNGSNRRRRRLEELGPVAFRLL
                     EEPAEIRAAIALMLRAKAAWMAARGLANPCITRPDFRSFLEEAALHPAACGSTRLMVL
                     DLCGQPAAIKLGTLDGTRFESFISTFDPALDAYSPGTLLQVESLRWCADHGLDYDMRI
                     GAEGYKADWATGDAPVTSHTIALTLRGVGWLWAQAADYQFRLRRDGLRDKLKAALAHR
                     REAAAEAPALAPGPWPVRGPTRGPAQG"
     gene            complement(1512..>2647)
                     /locus_tag="VFF98_08275"
     CDS             complement(1512..>2647)
                     /locus_tag="VFF98_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HZU63665.1"
                     /translation="PAPVPAPASTWQMDRAVVVMRHGIRPPTKAAALPAGMAPAPWPA
                     WDVGWGELTHHGAKAVALLGRFDGARYARLLGAGCPATGAVHAVADVDQRTRATAAAY
                     LAALKPGCQLAAESLADGAVDPRFSPFEANPLADAPTALASARAALPQGGEGALDRAL
                     AGQWHQIDAILDCHSPACSIASRPTALDVARGRVKLSGALALGGSFAETLALEYADGK
                     PLAEVGWGRAGRAQITQLLALHAGEFALTARPPVLARAGARALLAEVAGALTAARGPR
                     FSLFVGHDTNLAVLGGALGLHWQARQFAPDDPPPGAALLFERWHDGHGHYRLTIALRS
                     QSLDEMRDLTAPGAGAVTVLRQITCAGHRGCTTGALGAALGQP"
BASE COUNT          376 a          946 c          987 g          338 t
ORIGIN      
        1 ggcgcaaggg ggcctcaacc tggggcgcga ttatgccacg gccgcgctgg tggtggcgat
       61 cgcgctggcg ctgtggctgg tgccgcagcg ggccggcaag gcgcattgat cagccctgcg
      121 ctggcccccg tgtcggcccc cgtactggcc agggcccagg ggcgagggcg ggggcttcgg
      181 ccgccgcttc ccgcctgtgg gccagcgcgg ctttcagctt gtcccgcagg ccatcgcggc
      241 gcaggcggaa ctgatagtct gccgcctgcg cccacagcca gcccaccccg cgcagggtaa
      301 gggcgatggt gtggctggtg accggggcat cgccggtggc ccaatcggcc ttgtagcctt
      361 ccgcgccgat ccgcatgtcg taatcgaggc catgatcggc gcaccagcgc agcgattcca
      421 cctgcagcag cgtgccgggc gaataggcat cgagcgccgg atcgaaggtg gagatgaagc
      481 tttcgaagcg ggtgccatcc agcgtgccca gcttgatcgc cgccggctgg ccgcacaggt
      541 ccagcaccat cagccgggtg ctgccgcaag cggccggatg cagcgcggcc tcttcgagga
      601 agctgcggaa atcggggcgg gtgatgcagg ggttggccag gccccgcgcg gccatccagg
      661 ccgccttggc gcgcagcatc agggcaatgg cggcgcggat ctcggccggc tcctccagca
      721 ggcggaaggc caccgggccc agctcttcca gccggcggcg gcggcggttg ctgccattgc
      781 gcaggttctt gctgcggctt tgccaatagg cctgccagcc ggcaccatcg cgcaggcgca
      841 caaagggggc cggcagggtt tcgcgccttt gccacaggcc cttgcccaca aggctggccg
      901 cccgggcaga accttcgccc acaaacggca ccaccacccc atcggccggc agatgggccg
      961 cggcataggc ccaggcggcc gccagccagg cccccgcgcg cgggctggcg gcatgcagca
     1021 ggccatcata ttcggtgcca tcgctgccca gcgggcgggc aatgcggatg cccttctcgc
     1081 gccgcacttc cagcggccag acgagcacca gccgcccctc ctcgcgcagg gtcagcaccc
     1141 acaggcgcgc gccgcgcggg gccgcgcaga tgcgccagcc cgccagcgcc cactctgccc
     1201 cctgcgacag gcggggggca tcggccctgg cccacagcgc gttccattcc ggcgccaggg
     1261 cggccagcgc ctgcggatcg gtgatcgccg cgcattgcca tccgcttgcc tgggcggccg
     1321 ccagagcggg ggcgggcatg tggggcaggg cggaggcatg ggccggcatg ggcaactcgc
     1381 aacaggatgc atcagggcat gtgcggagca gcctacaagc gaaaggtaaa agattgatac
     1441 cggcgaaaag cagaggaaac aggaggccgc gcgagggctt tgcgcggcct cctgcccctg
     1501 cctcctgccc cttagggctg gcccagcgcg gcgccgaggg cgccggtggt gcagcccctg
     1561 tggccggcac aggtgatctg gcgcagcacc gtcacggcgc cggcgccggg cgctgtcaga
     1621 tcgcgcattt catccaggct ctggctgcgg agggcaatgg tcaggcgata atggccatgg
     1681 ccatcgtgcc agcgctcgaa cagcagggca gcgccgggcg gcggatcatc gggcgcgaat
     1741 tgccgcgcct gccagtgcag ccccagcgcg ccccccagca cggcgaggtt ggtgtcatgg
     1801 ccgacaaaca ggctgaagcg gggccctcgc gcggcggtaa gcgcccctgc cacctcggcc
     1861 agcagcgcgc gcgcgccggc gcgggccagc acgggcgggc gcgccgtcag cgcgaattcg
     1921 ccggcgtgga gggccagcaa ttgggtgatc tgcgccctgc cggcgcggcc ccagcccact
     1981 tcggccagcg gcttgccatc ggcatattcc agcgccagcg tttcggcaaa gctgccgccc
     2041 agcgccagcg cgcccgacag tttcacgcgg cctctggcca cgtccagtgc ggtgggccgg
     2101 ctggcgatgg aacaggccgg gctgtggcaa tcgaggatcg catcgatctg gtgccactgg
     2161 ccggccagcg cccggtcgag cgcgccttcg cccccctgcg gcagggcggc gcgggcgctg
     2221 gcaagcgctg tgggcgcatc agccagcggg ttcgcctcga agggcgagaa gcgcgggtcc
     2281 accgcgccat cggccaggct ttcggcggcc agctggcagc cgggcttcag cgcggccaga
     2341 taggcggccg ctgtcgcgcg ggtgcgctgg tcgacatcgg cgacggcatg cacggctcct
     2401 gtggccgggc agcccgcccc cagcagccgg gcatagcgcg cgccatcgaa gcggcccagc
     2461 agtgccacgg ccttggcgcc atggtgggtc aattcgcccc agcccacatc ccaggcgggc
     2521 cagggggcgg gcgccatgcc ggcgggcagg gcggcggcct tggtgggcgg gcggatgccg
     2581 tggcgcatca ccaccaccgc ccggtccatc tgccatgtgc tggcgggcgc aggaacgggc
     2641 gcggggg
//
LOCUS       DASRYE010000155         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_254572,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000155 DASRYE010000000
VERSION     DASRYE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254572"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFF98_08280"
     CDS             <1..337
                     /locus_tag="VFF98_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZU63666.1"
                     /translation="TVFNRYFPEKIPAVIERYTRESRRLLGVLDAALAHSDYLAGDYS
                     IADIACFPWVRGHDWAGIDISGLDNLQRWFDAIAARPAAQRGLLIPTPPTAQEMAAKT
                     TTQGKAILA"
     gene            334..1749
                     /locus_tag="VFF98_08285"
     CDS             334..1749
                     /locus_tag="VFF98_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63667.1"
                     /translation="MNELPGVAPRTIALVVATVMFMGILEGTIIATALPHMARDFAVA
                     PVDLSAGITAYLLVSAVFLPISSWVAGKLGTRRVLVVAIAGFAGSSLLCGLCTGLPAF
                     VAARGLQAMFSALMVPVGNLVLLRVTPKRDLVEMTAISTTPALIAPVIGPPVGGLITA
                     ALGWRYIFFVNVPLAILGIALVLKLVPNLREEKAQTFDTRGFVLVAAALSAAVYALDR
                     IGAAPMQWRGPAALAVLASVAGWLAMRHMGRAPAPLVPLDALRHGTFRSVAFGAGFLM
                     RLPFMGQALLLPLFFQVGFGLSPFATGMLLLAQNTGDLMLKPVAGRALRRMGFRTALT
                     LGTATMMSAMGATALLQPYWPLWVLAGAMAITGMARSIAFTGMMSLSFADVDRHELAG
                     ATVINNLATSLSAAAGISLAALVVNAMAGGAVANLADYREAIAVLAGVGALAVPLFAR
                     LPRDAGAAVSGHAVPAGLDHF"
     gene            complement(1751..>2044)
                     /locus_tag="VFF98_08290"
     CDS             complement(1751..>2044)
                     /locus_tag="VFF98_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZU63668.1"
                     /translation="FKWDYSRTAQPEVFDVLIAEMREASDMFRECWEESEIRTHFEDV
                     NTATLPEIGEIAFQHTSYAIEEVPGQRLILFAPADAASAAKLRRLADGLAPNL"
BASE COUNT          261 a          699 c          713 g          371 t
ORIGIN      
        1 caccgtcttc aaccgctact ttcccgagaa gatccccgcc gtgatcgagc gctacacccg
       61 cgagagccgc cgcctgctgg gcgtgctcga cgcggcgctg gcccacagcg actatctggc
      121 cggcgactat tccatcgccg acatcgcctg cttcccctgg gtgcgcgggc acgactgggc
      181 cggcatcgac atctcggggc tggacaatct gcaacgctgg ttcgacgcga tcgccgcgcg
      241 cccggccgcg cagcgcggcc tgctgatccc cacgcccccc accgcgcagg aaatggccgc
      301 caagaccacc acgcaaggca aggcgatcct ggcgtgaacg aactgcccgg cgtggccccg
      361 cgcaccattg cactggtcgt ggccacggtc atgttcatgg gcattctgga aggcacgatc
      421 attgccaccg ccctgccgca catggcgcgc gatttcgccg tggcgccggt cgatctgtcg
      481 gccggcatca cggcctatct gctggtttcg gcggtgttcc tgcccatcag cagctgggtg
      541 gcgggcaagc tgggcacaag gcgggtgctg gtggtggcca tcgccggttt tgccggctcg
      601 tcgctgctgt gcgggctgtg cacggggctg cccgcctttg tcgcggcgcg cgggcttcag
      661 gccatgttct cggcgctaat ggtgccggtg ggcaatctgg tgctgctgcg cgtcacgccc
      721 aagcgcgacc tggtcgagat gaccgcgatc agcaccaccc cggccctgat cgcgccggtc
      781 atcgggccgc cggtgggcgg gctgattacc gccgcgctgg gctggcgcta catctttttc
      841 gtgaatgtgc cgctggccat tctgggcatc gcgctggtgc tgaagctggt gcccaacctg
      901 cgcgaggaaa aggcgcaaac ctttgatacg cggggcttcg tgctggtggc agcggcgctt
      961 tcggcggcgg tctatgcgct cgaccggatc ggcgcggcgc caatgcagtg gcgcggaccg
     1021 gccgcgcttg ccgtgctggc cagcgttgcc ggctggctgg ccatgcgcca catgggccgc
     1081 gcgcccgcgc cgctggtgcc gctcgatgcg ctgcggcacg gcacattccg ctcggtcgcc
     1141 tttggcgcgg ggttcctgat gcggctgccg ttcatggggc aggcgctgct gctgccgctg
     1201 ttctttcagg tcgggtttgg cctgtcgccc tttgccaccg gcatgctgct gctggcgcag
     1261 aacacgggcg acctcatgct caaacccgtg gcggggcgtg ccctgcgccg catggggttc
     1321 cgcacagccc tcactttggg caccgccacg atgatgagcg ccatgggcgc caccgccctg
     1381 ttacagccct attggccgct gtgggtgctg gccggcgcca tggcgatcac cggcatggcc
     1441 cgctcgatcg cctttaccgg catgatgtcg ctgagctttg ccgatgtcga ccggcacgaa
     1501 ctggccggcg ccacggtcat caacaatctg gccacctcgc tctcggcggc ggcaggcatt
     1561 tcgctggccg cgctggtggt caacgccatg gcgggcgggg cggtggccaa cctggccgat
     1621 taccgcgagg cgattgccgt gctggccggt gtgggcgcgc tggcggtgcc gctgttcgcg
     1681 cgcctgccgc gcgacgcggg cgcggccgta agcgggcacg ccgtgcccgc cggcttggac
     1741 cacttctagc ctagaggttg ggcgccagac cgtcggccag gcggcgcagc ttggcggcgc
     1801 tggcggcatc ggccggggca aacaggatca gcctttgccc cggcacttcc tcgatggcgt
     1861 aagaggtgtg ctggaaggcg atctcgccaa tctcgggcag ggtggcggtg ttcacatcct
     1921 cgaaatgggt gcggatttcc gattcctccc agcactcgcg gaacatgtcg gaggcctcgc
     1981 gcatctcggc gatcagcacg tcgaacactt cgggctgggc ggtgcggctg tagtcccact
     2041 tgaa
//
LOCUS       DASRYE010000156         6225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_95945,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000156 DASRYE010000000
VERSION     DASRYE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95945"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFF98_08295"
     CDS             complement(<1..180)
                     /locus_tag="VFF98_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZU63669.1"
                     /translation="MAGIGQEAADLLAARVEAFVRSEVIAYESDPRRDHHGAPTDELV
                     MELREKARAAGVLTPH"
     gene            complement(202..969)
                     /locus_tag="VFF98_08300"
     CDS             complement(202..969)
                     /locus_tag="VFF98_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63670.1"
                     /translation="MEINGLAAIVTGGASGLGAASAERLAAGGAKVTIFDLNAELGAA
                     KAREIGGHFVAVNVTDPANVEGAIAEAEGLHGKARILVNCAGIGPPAKVLNRDGSPLP
                     LEDFARIININLIGTFNVLSKFAARIFEAEPLNADGERGVIINTASVAAFDGQIGQPA
                     YAASKGGVVAMALPIAREFARYGIRVNTIAPGIFWTPLLATLPQEAQDSLGKQVPFPS
                     RLGQPAEFARLVAAMIDNPMLNAEVVRLDGAIRMAPK"
     gene            complement(989..2122)
                     /locus_tag="VFF98_08305"
     CDS             complement(989..2122)
                     /locus_tag="VFF98_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63671.1"
                     /translation="MILSDIQIAVQDTVRAFARERLRPQAAQHAAQGGYPADLFADLA
                     GLGLMGMTAPEAVGGAGVDYVSYALALIEIAAGDGALSTILSIHNSLIVSALLRFGSA
                     AQQQRFLPGLIAGQHTGAFALTEAEAGSDAAALRTRATPVPGGWRLNGAKQFISNGRI
                     ARLAIVFAVSDPAAGKKGISAFLVPTDSAGYVVDKVEHKLGQTASDTCAIRFDDLFVP
                     DALVLGTPGEGYRIALANLEAGRIGIAAQCIGMADAALAIAIGYAKERHSFGQPIISH
                     QAVGHRLADLAARLEAARQLVLHAAAVKDSGASCLKEASMAKLVASETAEAVVSGAIQ
                     TLGGYGYLEDYGVARIYRDVRVCQIYEGTSDIQRMVIARDLAA"
     gene            2212..2829
                     /locus_tag="VFF98_08310"
     CDS             2212..2829
                     /locus_tag="VFF98_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63672.1"
                     /translation="MSSAPSPFQTAQQRRSSRQAKRDAVLRAAVRMFNERGFHQTSLD
                     DVAAQLGVSKPTIYHYLGNKDQVLLECVTIGLSQLLEAAETSRQKPGKGVDRLIAFLT
                     RYAEINMDDFGRCVIRTSDEALGPDSRQQFHALKRRIDTDLRQLIRDGMEDGSIAPTD
                     PKMLAFALAGALNWPARWHNPQGADTPEQIARQLVLVLAQGFLPR"
     gene            complement(2856..3956)
                     /locus_tag="VFF98_08315"
     CDS             complement(2856..3956)
                     /locus_tag="VFF98_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZU63673.1"
                     /translation="MQITAALAVAPGAPFELAELELAAPGPGEVLVRIAGVGLCHTDL
                     VFRDQFIPYPLPAVLGHEGAGVVAALGPDVQGLAVGDTVVLGFASCGACPRCAQQLPS
                     CCREFVARNYAGVRPCGTSPLSLHGVAVAGAFFGQSSFASHALVNWRNVVRVEAGADL
                     ALLGPLGCGLQTGAGAVLRAMDCPVGSTLVVFGGGPVGLAGVMAARYRGCAQIVLAEP
                     LAARRALALELGASHVLDPAAGDMAAQIRQILPDGADFVLETSGREAVVAEAMACLAS
                     HGTLGLVGVPARADSTLAVNLAGLITHGHRIMGIVEGDSDLFTFIPELVALYRAGHFP
                     FDRLIQTFPLAEINAAIAAQHRGDCVKAVLLP"
     gene            complement(3968..4771)
                     /locus_tag="VFF98_08320"
     CDS             complement(3968..4771)
                     /locus_tag="VFF98_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63674.1"
                     /translation="MTMTQQQDLAGKLAWVTGAGSGIGRAVAVALAGAGAQLVLTDID
                     QAAAEACAALAGPQAIALPQDVSAQQDWQAVAATIAEDFGQLDILVNNAGVMLTAPLE
                     SAPLDHLRRQYAINVESVFIGMQTAYPLMRRALAAGGRDAAIVNISSVYGMVAGGSFA
                     AYSASKGAVRGLSRAAALEWAQSGIRVNCVLPGPVQTNLGAHWDPPLDAAGQPLAPEA
                     ALAAWVALIPMGRLGQAQEIAPLVAFLASEAAAFITGAEFVADGGYTAA"
     gene            complement(4768..5181)
                     /locus_tag="VFF98_08325"
     CDS             complement(4768..5181)
                     /locus_tag="VFF98_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZU63675.1"
                     /translation="MAHGHSGGCLCGAVRYRVAGDPLMVGICHCRHCQKQSGAPFSVV
                     WAVAESAFAATGAIAQYADSGDSGQAVYRQFCGACGSPIRSLAAAMPGVCFVKAGTLD
                     APGRFAPTFEVYCARGWPWLPALAAQRHPLALGDA"
     gene            complement(5196..>6225)
                     /locus_tag="VFF98_08330"
     CDS             complement(5196..>6225)
                     /locus_tag="VFF98_08330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU63676.1"
                     /translation="SRREPVGVVAAITPWNFPLVLAMWKIAPALAAGCTLILKPAAET
                     PLSALRLAELARQVGFPPGVFTVLTGDGATGAALAGHAGIAKVAFTGSTATGRAILAA
                     SIPDFKRVTLELGGKSPSIVCADADLARAIPQVAMGCFFNAGQLCYAGARAYVHRSVY
                     EPVLEGLAAVAAGQKLGLSSARDSQIGPLISARQHQRVAGFVARARAAGIAQVPGAGA
                     APLPSTGHFFAPTVLRDVPPDAEIAREEVFGPVLAVSPFDDLDEAIALANDSPYGLAA
                     HVWTRDLACAHHAAARLEAGTVFTNCILLADPAFPFGGRKASGLGRENGAEVLDAYLE
                     PKTVVMAL"
BASE COUNT         1047 a         2257 c         2039 g          882 t
ORIGIN      
        1 atgcggtgtc agcacacccg ccgcccgcgc cttctcccgc agctccatca ccagctcatc
       61 cgtcggcgca ccatgatgat cccgacgcgg atcgctctca taggcaataa cctcggagcg
      121 gacgaaggct tctacccgtg ccgccagcag gtctgccgct tcctggccaa tgccggccat
      181 gttcatatcc tccgccatgg cttacttcgg cgccatgcga atggcgccat ccagccgcac
      241 gacctcggca ttgagcatgg ggttgtcgat catcgcggcc accagccggg caaattccgc
      301 cggctggcca aggcggctgg ggaacggcac ctgcttgccc agcgaatcct gcgcttcctg
      361 cggcagggtg gccagcagcg gggtccagaa gatgcccggc gcaatggtgt tcacgcggat
      421 gccatagcgg gcaaattcac gcgcgatggg cagcgccatg gccaccacgc cgcccttgct
      481 ggcggcatag gcgggctggc cgatctggcc gtcgaaggcc gccacgctgg cggtgttgat
      541 gatcacaccc cgctcgccat cggcgttcag cggctcggcc tcgaagatgc gcgcggcgaa
      601 tttcgagagc acgttgaacg tgccgatgag gttgatgttg atgatccgcg cgaaatcctc
      661 cagcggcagc gggctgccat cgcggttcag caccttggcc ggtgggccga tgccggcgca
      721 gttgacaagg atgcgcgcct tgccatgcag gccctcggcc tcggcgatgg cgccttccac
      781 attagccgga tcggtgacgt tgaccgccac gaaatggccg ccgatctcgc gcgccttggc
      841 cgcgcccagt tcggcattga gatcgaagat cgtcaccttc gcgccgccgg cggccaggcg
      901 ctcggcgctg gccgcgccca gcccggaggc cccgcccgtg acgatggccg ccaatccgtt
      961 gatttccatg acatgctctc cttgattgtc aggccgccag atcccgcgcg atgaccatgc
     1021 gctggatatc ggacgtgccc tcgtagatct ggcagacgcg cacatcgcgg tagatgcggg
     1081 cgacgccgta atcctccaga tagccatagc cgcccagcgt ctggatcgcg ccggagacga
     1141 ccgcctcggc ggtttcggaa gcgaccagct tggccatcga ggcctccttc aggcaggagg
     1201 cgccgctgtc tttcacggcg gcggcatgca gcaccagctg gcgcgccgct tccagccggg
     1261 cggccagatc cgccagccgg tggcccacgg cctgatggct gatgatcggc tggccgaagc
     1321 tgtggcgctc ctttgcatag ccgatggcga tggccaacgc ggcatcggcc atgccgatgc
     1381 actgggcggc aatgccgatg cgcccggctt ccagattggc cagggcaatg cgatagccct
     1441 cgccgggcgt gcccagcacc agcgcatcgg gcacgaacag atcgtcgaag cggatggcgc
     1501 aggtgtcgga agcggtctgg cccagcttgt gctccacctt gtccaccaca tagccggcgc
     1561 tatcggtggg caccaggaag gccgagatgc ccttcttgcc cgccgccgga tcgctgaccg
     1621 caaagacgat cgccagccgg gcgatgcggc cattggaaat gaactgcttg gccccgttca
     1681 gccgccagcc gccgggcacg ggcgtggccc gcgtgcgcag cgctgccgca tccgatccgg
     1741 cctcggcctc ggtaagggca aaggcgccgg tgtgctggcc ggcgataagc cccggcagga
     1801 agcgctgctg ctgcgccgcg ctgccaaaac gcagcagcgc cgagacgatc aggctgttgt
     1861 ggatggaaag gatggtggaa agggcgccat cgcccgcggc aatttcgatc agcgccagcg
     1921 cataggacac ataatccacc ccggcgccgc ccaccgcttc cggcgcggtc atgcccatca
     1981 gccccagccc tgccagatcc gcgaacagat ccgccggata gcccccttgc gcggcatgct
     2041 gcgcggcctg cgggcgcagc ctttcacggg caaaggcgcg cacggtgtcc tgcacggcga
     2101 tctggatgtc ggagagaatc ataaaaccca ccagtcaatt gtctactcat gagtagattg
     2161 tattcgacgc agtttcgtgt caagagcaag ccgtcttcac aggagagatt tttgagcagc
     2221 gccccttccc ccttccagac cgcccagcag cgccgttcca gccggcaggc caagcgcgat
     2281 gcggtgctgc gcgcggccgt gcggatgttc aacgaacgcg gctttcacca gacctcgctg
     2341 gatgatgtgg cagcgcagct gggcgtctcg aagccgacga tctaccatta cctgggcaac
     2401 aaggatcagg tgctgctgga atgcgtgacg atcggcctgt cgcaattgct ggaggcggcc
     2461 gagacctcgc gccagaagcc cggcaagggg gtggaccggc tgatcgcctt cctcacccgc
     2521 tatgccgaga tcaatatgga tgatttcggc cgctgcgtga tccgcaccag cgacgaggcg
     2581 ctgggcccgg acagccgcca gcagttccac gcactgaagc ggcggatcga caccgacctg
     2641 cgccagctga tccgcgatgg catggaggat ggctcgatcg cgccgaccga tcccaagatg
     2701 ctggcctttg cgctggccgg cgcgctgaac tggccggcgc gctggcacaa cccgcaaggc
     2761 gccgacacgc cggaacagat cgcccggcag ctggtcttgg tgctggcgca aggctttctt
     2821 ccccgctgac cgggcccgct gatggcgcct gctgatcagg gcaacagcac cgccttcacg
     2881 caatcgccgc ggtgctgggc ggcgatggcg gcgttgatct cggccagggg gaaggtctgg
     2941 atcagccggt cgaaagggaa atggccggcg cgataaagcg ccaccagctc ggggatgaag
     3001 gtgaacaggt cgctgtcccc ctcgacaatg cccatgatcc ggtggccatg ggtgatcagc
     3061 ccggccaggt tcaccgccag cgtgctgtcg gcgcgcgcgg gcacgcccac cagccccagc
     3121 gtgccatggc tggcaaggca ggccatggcc tcggccacca ccgcctcgcg cccgctcgtc
     3181 tccagcacga aatcggcgcc atcgggcagg atctggcgga tctgcgctgc catgtcgccc
     3241 gcggccgggt ccagcacatg gctggccccc agctccagcg ccaaagcgcg gcgcgcggcc
     3301 agcggctctg ccagcacgat ctgcgcgcag ccgcgatagc gcgccgccat cacgcccgcc
     3361 agccccaccg gcccgccgcc aaacaccacc agcgtgctgc ccacagggca atccatcgcc
     3421 cgcagcacag cgccggcgcc ggtttgcaag ccgcagccca gcgggcccag cagggcgaga
     3481 tccgcccccg cctccacccg caccacattg cgccagttca ccagcgcatg gctggcaaag
     3541 gaggattgcc cgaagaaagc ccccgccacc gccaccccgt gaaggctgag cgggctggtg
     3601 ccacagggcc gcacgccggc ataattgcgc gccacaaatt cgcggcaaca ggagggcagc
     3661 tgctgggcgc agcgcgggca ggccccgcag ctggcaaagc ccagcaccac cgtatcgccc
     3721 accgccagcc cctgcacatc cgggcccagc gccgccacca cgccggcccc ctcatgcccc
     3781 agcaccgccg gcagcggata ggggatgaac tgatcgcgaa agaccagatc ggtgtggcac
     3841 aggcccacgc cggcaatccg caccagcacc tcgccggggc cgggcgcggc cagctccagc
     3901 tcggccagtt cgaaaggcgc gcccggcgcc accgccagcg cggccgtgat ctgcatcgcc
     3961 ccctccctca tgccgccgta tagccgccat cggcgacgaa ttcggcgccg gtgatgaaag
     4021 ccgccgcctc gctggcaaga aaggccacca gcggggcgat ctcctgcgcc tggcccagcc
     4081 ggcccatggg gatcagcgcc acccaggccg ccagcgccgc ttctggcgcc aggggctggc
     4141 cggcggcatc gagcggcgga tcccagtggg cgccaaggtt ggtttgcaca gggcccggca
     4201 gcacgcaatt cacccggatg ccgctttgcg cccattccag cgccgccgcg cgcgacaggc
     4261 cccgcaccgc ccctttcgac gcgctgtagg cggcaaagct gccccctgcc accatgccat
     4321 agaccgagga gatgttgacg atggccgcat cgcgcccgcc cgccgccagc gcccggcgca
     4381 tcagcggata ggccgtctgc atgccgatga acacgctttc cacattgatc gcatattgcc
     4441 ggcgcagatg gtcaagcggc gcgctttcca gcggcgctgt caacatcacc ccggcattgt
     4501 tgacgagaat gtcgagctgg ccgaaatctt cggcaatggt ggcggccacc gcctgccaat
     4561 cctgctgcgc ggacacatcc tgcggcaggg cgatcgcctg cgggccggcc agcgccgcgc
     4621 aggcctcggc cgcggcctgg tcgatatcgg tgagcaccag ctgcgcgccg gcgccggcca
     4681 gcgccaccgc caccgcccgg ccaatgccgg agcccgcccc cgttacccag gcaagcttgc
     4741 ctgccagatc ctgctgctgt gtcatggtca tgcgtctcca agggccaggg gatgtctttg
     4801 ggcggcgagc gccggcaacc acggccagcc gcgcgcgcaa tagacctcga atgtgggggc
     4861 aaagcggccg ggcgcatcca gcgtgccggc cttcacgaag cagacgccgg gcatcgccgc
     4921 ggccagcgag cggatcggcg agccgcaggc gccgcagaac tggcgataga ccgcctgccc
     4981 gctatcgccg ctgtcggcat attgggcgat ggcgccggtg gccgcaaagg cgctttcggc
     5041 cacggcccac accaccgaga agggcgcgcc cgattgcttc tggcaatggc ggcaatggca
     5101 gatgcccacc atcagcggat cgcccgccac ccggtagcgc accgcgccgc acaggcagcc
     5161 gccgctatgg ccgtgtgcca tgctgtcctc cccgttcata gcgccatcac caccgtcttg
     5221 ggctccagat aggcatccag cacttcggcg ccgttttccc gccccagccc cgatgccttg
     5281 cgcccgccaa agggaaaggc gggatctgcc agcaggatgc aattggtgaa caccgtgccc
     5341 gcttccagcc gggcggcggc atggtgggcg caggccagat cgcgcgtcca cacatgggcg
     5401 gccaggccat aggggctgtc attcgccagc gcgatcgcct cgtcgaggtc gtcgaacggg
     5461 ctgacggcca gcaccgggcc aaacacttcc tcccgcgcga tctcggcatc gggcggcaca
     5521 tcgcgcagca cggtgggggc gaagaaatgc ccggttgacg gcagcggggc tgcccctgcc
     5581 cccggcacct gcgctatgcc ggccgcgcgg gcgcgggcca caaagcctgc cacgcgctgg
     5641 tgctggcggg cgctgatcag cgggccgatc tggctgtcgc gcgcagagct gaggcccagc
     5701 ttctggccgg ccgccaccgc cgccagccct tccagcaccg gctcgtagac cgaacggtgc
     5761 acataggccc gcgcccccgc atagcacagc tggccggcgt tgaagaaaca gcccatcgcc
     5821 acttgcggga tcgcccgcgc cagatccgca tcggcgcaga cgatggaagg cgatttgccg
     5881 cccagctcca gcgtcacccg cttgaaatcg gggatgctgg cggcaaggat ggcgcggccc
     5941 gtggcggtgg agccggtgaa ggccaccttg gcaatgccgg catggccagc cagcgccgca
     6001 ccggtggcgc catcgcccgt cagcacggtg aagacgccgg gaggaaagcc cacctgccgg
     6061 gccagttcgg ccaggcgcag ggccgatagc ggcgtttcgg ccgccggctt caggatcagc
     6121 gtgcagcccg ccgccagcgc cggggcaatc ttccacatcg ccagcaccag cgggaaattc
     6181 cacggggtga tcgccgccac cacgcccacc ggctcgcgcc gggaa
//
LOCUS       DASRYE010000157         6588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_24398,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000157 DASRYE010000000
VERSION     DASRYE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24398"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VFF98_08335"
     CDS             <1..1960
                     /locus_tag="VFF98_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU63677.1"
                     /translation="PRQADPMGTNPVVPGLPADTYVVDTQTRLIGSPQVALRVVRQLH
                     LANLPAYAKGDLTPPAGPLSQTDATPEEARAVVNLLKKVDVRRSGLTYVIDVAVKAPT
                     AELAAQIANGFAQSYIDLGNEAKRNTTANAARYVADNATHLQAQALADDEALHSYMSA
                     HNLMSADGITLAERQISDLSTQMAQAKATLAQEQGKYNAARQQVQHGDGADVNEVLSS
                     ETIKQLRTQEAQVSGDLATLQGRYGSRYPDVISARGSLADIHRHIEAERDRILSGLKA
                     NVEVARTGLSSLQSSYGQLQTALVANNAALVGMQDLQRKADASKQVYSAFLERAKETA
                     AQAQAPAPDASIAAAARVPALPAWPNPLLAALIGLSAAGLAGLIAIAIAEYLDTSVRS
                     RSEMERQVGIAYAGALPDVRRTSRDAGEHPQDYLINRPFSLFAEALRNLGAFLALRGN
                     GNARVIAITSSLPREGKTTLAVCLGRALARSGRRVILVDGDGRRHSASNLLAGTRAPG
                     KGIEDVLAGSATLPEAIVQDEQSGLFLLPSRGASADAVEITPDMASHLFTALRQDFEW
                     VIVDTAPVLGLAEARVLASAADAVLLVVRWRKTAGRAAQAAVQVLQECRAQLVGGVLS
                     MVDSRQFAATADGDSYLYHGTYKNYYVN"
     gene            2066..3814
                     /locus_tag="VFF98_08340"
     CDS             2066..3814
                     /locus_tag="VFF98_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZU63678.1"
                     /translation="MRACRKIFVDAAIFSAFLNLLYLAPTIYMLQVYDRALPSRGETT
                     LGLLTVIFVAATATIATLDLVRTRLLGAASARLDRLLVGQILDALMRVARQPGSGRNT
                     ATLREFDNLRQTLTGIGVLALFDVPWAPIYIIACLLLHWALGLLALTGCLLLGLFSWL
                     AERSTKQGYSQANQLSQRNFMGMDASLSSAGVIWALGMREAMVARHLRERGEATTLQA
                     RAGYRGAAYTSYVKALRLLMQSLALGLGAWLAIEQEISAGAIFAASLLVSRALAPIEM
                     VTGAWRGLQQARDAYDALKTLFEECGEPRQPTALPAPTGKVAVEGLSVASPQRDRLLL
                     QNISFGLAAGEMLGVIGPSGAGKSTLVKAMVGIMPVVGGKVRIDGAALDDWPEEQLAS
                     IIGYVPQEPTLYRGTIKENIARFETETAKDRTVIDAEVVRAAQMCGAHDFILRLPHGY
                     DTELGWGGAGLSGGQAQRVTVARALYNRPQVLLLDEPNANLDAEGAAALVSALAALKT
                     QGITIILVAHNTGILAHVDKLLVIKDGRVEMFGTRQQVVQRLSGQAPDAPPAPPPPAE
                     GPPPADTPAPHDQPAI"
     gene            3829..5208
                     /locus_tag="VFF98_08345"
     CDS             3829..5208
                     /locus_tag="VFF98_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZU63679.1"
                     /translation="MTSDVTILDAEHRLLPALPQGHADSEAEAGANARLGWVVVVLFF
                     GVFLGFASFFRLDAAANADGVVKVSGERQTVQHRAGGTVAALFVREGQQVKAGDVLIL
                     LAGAEVAANEQSLAAQVVDLMAERARLQAELNSSAAVIPPPEFATLPAEDKPLAEAAL
                     RQQIHQFQQNRVALTTQKRVVAQQAAQLQEKASGLGEQMGANRQEDSSYAAQLDGMRE
                     LAAQGYASVNRVRELERARQQMNGDFARLSADRASTRNQIGEMRMRSLSIDTDDRRQV
                     AEDLRKTVDELNAALPKWEDARQQVENTRIRAPVGGQVVGLSIFTVGGVITPGEKLMS
                     IVPSKATLVVEGHVQPTDANDLVVGQKAQVRFPGFHERSLPQIEGTVTRVSADAFTDD
                     HTGKSYFTIEVTVPPADMAKLKPFAAGGLGLRPGLPATVLVPLRRRTLMGYLLEPLNQ
                     ALWRSGREH"
     gene            5277..>6588
                     /locus_tag="VFF98_08350"
     CDS             5277..>6588
                     /locus_tag="VFF98_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HZU63680.1"
                     /translation="MLFGSLVFVFAFLPVVLLAYHALRGAGHERTAKYFLVAASLFFY
                     GWWDPRYVPLVSLSIGANFALSLLMERQPGRASLLLGLGVAGNLALLGYYKYAHFFVT
                     ALAGATGLPWHRGRIVLPLAISFFSFQQIAYLIESRRDGKAAHSLLDYALFVLFFPHL
                     IAGPITHHKEMLPQFAAMGRHRLPPSYMPIGTAIFIMGLAKKVVVADLLAMLANPVFN
                     AARDGLALAPWQAWQGALAYTLQLYFDFSGYSDMAIGLGLMFGILLPVNFASPYKSTS
                     IVEFWRRWHISLSRFLRNYLYFSLGGNRAGPVRRYANLLATMLLGGLWHGAGWTFIAW
                     GGLHGSYLVINHLWRGRFGPARGPVGRLAGWGLTMLAVVVAWVLFRADTFASAVAVLR
                     AMAGAPLAGGASGYLVPQLLGWAAVALAGGVALFAPNVLEMAGFP"
BASE COUNT         1037 a         2194 c         2257 g         1100 t
ORIGIN      
        1 gccgcgccag gcagacccga tgggcaccaa tccggtggtg ccggggctgc cggctgacac
       61 ctatgtcgtc gacacgcaaa cgcggctgat cggcagcccc caggtggcgc tgcgcgtggt
      121 gcgccagctg catctggcca acctgccggc ctatgccaag ggcgatctca ccccgccggc
      181 cggcccactt tcgcagacag acgccacgcc ggaggaagcg cgcgcggtcg tcaacctgct
      241 gaagaaagtg gatgtgcgcc gctcgggcct gacctatgtg atcgatgttg ccgtcaaggc
      301 gccaacagcc gaactggcgg cgcaaatcgc caatggcttt gcgcaaagct atatcgacct
      361 tggcaatgag gccaagcgca acaccaccgc caatgccgcc cgctatgtgg ccgacaatgc
      421 cacccatctg caggcccagg ccctggccga cgacgaagcg ctccacagct atatgagcgc
      481 gcataatctg atgagcgcgg atggcatcac gctggccgag cgccagattt ccgacctgtc
      541 gacacagatg gcccaggcca aggcgacgct ggcgcaggaa cagggcaagt ataatgccgc
      601 gcgccagcag gtgcagcatg gcgatggcgc cgatgtgaac gaggtgctct cgtccgagac
      661 gatcaagcaa ctgcgcacgc aggaagcgca ggtcagcggc gatctggcca cgctgcaggg
      721 ccgctatggc agccgctatc ccgatgtgat cagcgcgcgc ggcagcctgg ccgatatcca
      781 ccgccacatc gaggccgagc gtgaccgcat cctctcgggc ctgaaggcca atgtggaagt
      841 ggcgcggacc gggctgtcct ctctgcaatc cagctatggc cagctgcaga ccgcgctggt
      901 ggccaacaat gccgccctgg tgggcatgca ggatctgcag cgcaaggccg atgccagcaa
      961 gcaggtctac agcgccttcc tcgaaagggc gaaggaaacc gcggcccagg cccaggcgcc
     1021 ggcgccggat gccagcattg ccgcagccgc ccgcgtgccc gccctgccgg cctggcccaa
     1081 cccgctgctg gccgccttga tcgggctgag cgcggccggc cttgccgggc tgatcgccat
     1141 cgccattgcc gaatatctgg acaccagcgt gcgttcgcgc agcgagatgg agcggcaagt
     1201 gggcatcgcc tatgccggcg ccctgcccga tgtgcgccgc accagccgcg atgccggcga
     1261 acacccgcag gactatctga tcaaccgccc cttctcgctg tttgccgagg cgctgcgcaa
     1321 tctgggggcc tttctggccc tgcgcggcaa tggcaatgcg cgggtgatcg ccatcacctc
     1381 ctccctcccg cgtgagggca agaccacgct ggcggtgtgc ctggggcggg cgctggcgcg
     1441 ttcgggccgg cgagtgatcc tggtggatgg cgacggccgg cggcacagcg cctcgaacct
     1501 gctggcgggc acgcgcgcgc cgggcaaggg cattgaggat gtgctggccg gcagcgcaac
     1561 cctgccagag gccatcgtgc aggacgagca gagcggcctg ttcctgctgc cctcgcgcgg
     1621 ggcgtctgcc gatgcggtgg agatcacgcc ggatatggcc agccacctgt tcaccgcgct
     1681 gcgccaggat ttcgagtggg tgatcgtgga tacagcaccg gtgctggggc tggccgaagc
     1741 ccgggtgctg gccagcgccg ccgatgccgt gctgctggtg gtgcgctggc gcaaaaccgc
     1801 cgggcgcgcg gcccaggccg ccgtgcaggt gctgcaggaa tgccgcgcgc agctggtggg
     1861 cggcgtgctc tccatggtgg acagccgcca gtttgccgcc accgccgacg gggactccta
     1921 tctctatcac ggcacctaca agaattatta cgtgaactga gcaggttctg ccctttgcgg
     1981 aaagccatgc accatgacct cactcaccag atctgccgct cagccggttc cgctttccag
     2041 ggcttcgggc attttccggg aagccttgcg ggcatgccgc aagatcttcg ttgatgcggc
     2101 gatcttcagc gcctttctca acctgctcta cctcgcgccg accatttaca tgctgcaggt
     2161 ctacgacagg gccctgccct cgcgcgggga gacgacgctg ggcctgttga cggtgatctt
     2221 cgtggcggcc accgccacta tcgccacgct ggatctggtg cgcacgcggc tgctgggcgc
     2281 ggccagtgcc cggctggacc ggctgctggt ggggcagatc ctcgatgcgc tgatgcgcgt
     2341 ggcgcgccag cccggcagcg ggcgcaacac cgccaccctg cgcgagttcg acaatctgcg
     2401 ccagacgttg accggcattg gcgtgctggc cctgttcgac gtgccctggg cgccgatcta
     2461 tatcatcgcc tgcctgctgc tgcattgggc gctgggcttg ctggcgctga ccggctgcct
     2521 gctgctgggc ctgttcagct ggctggcaga gcgcagcacc aagcagggct attcccaggc
     2581 caaccagctg tcgcagcgca atttcatggg gatggacgcc tcactctcca gcgccggggt
     2641 gatctgggcg ctgggcatgc gcgaggcgat ggtggcaaga cacctgcgcg agcgcggcga
     2701 ggccaccacg ctgcaggccc gcgccggcta tcgcggcgcg gcctacacct cctatgtgaa
     2761 ggcgctgcgc ctgttgatgc aatcgctggc gctggggctg ggcgcctggc tggcgatcga
     2821 gcaggagatt tccgccggcg cgatctttgc ggcctcgctg ctggtctcgc gcgcgctggc
     2881 gccgatcgag atggtcaccg gcgcctggcg cggcctgcaa caggcgcgcg acgcctatga
     2941 cgcgctgaaa accctgttcg aggaatgtgg cgagccgcgc cagcccaccg cccttcccgc
     3001 gcccaccggc aaagtggcgg tggaaggcct gtctgtggca tcgccgcagc gggaccggct
     3061 gctgctgcaa aacatcagct ttggactggc cgcgggcgag atgctggggg tgatcggccc
     3121 cagcggcgcg ggcaaatcca cgctggtgaa ggccatggtg ggcatcatgc cggtggtggg
     3181 cggcaaggtg cgcatcgatg gcgccgcgct ggatgattgg ccggaagagc agctggccag
     3241 catcatcggc tatgtgccgc aggagccgac gctctatcgc ggcacgatca aggaaaacat
     3301 cgcccgcttc gaaacagaga ccgccaagga ccgtaccgtg atcgatgccg aggtggtgcg
     3361 ggcagcgcaa atgtgcggcg cgcatgactt catcctgcgc ctgccccatg gctatgacac
     3421 ggaactgggc tggggcggcg cggggctgtc tggcgggcag gcgcagcgag ttacggtggc
     3481 gcgcgcgctc tacaaccggc cgcaggtgct gctgctggac gagcccaacg ccaacctcga
     3541 tgccgaaggc gccgcagccc tggtcagcgc gctggccgcg ctgaaaacgc aagggatcac
     3601 catcattctg gtggcgcata acaccggcat ccttgcccat gtggacaagc tgctggtgat
     3661 caaggatggc cgggtggaaa tgttcggcac gcgccagcag gtggtgcagc ggctgagcgg
     3721 ccaggccccc gatgcccccc ctgccccgcc gccgcccgcg gaaggccccc cgccggccga
     3781 cacccccgcc ccccacgacc agccggcgat ctgagccagg agcaggccat gactagcgat
     3841 gtcaccattc tcgatgcgga acaccgcctg ctgccggccc ttccccaggg ccatgccgat
     3901 agcgaggccg aggccggcgc caatgcgcgg ctgggctggg tggttgtggt gctgttcttc
     3961 ggcgtgttcc tgggctttgc ctccttcttc cggctggatg cggcggccaa tgccgatggc
     4021 gtggtgaagg tttcgggaga gcgacagacg gtgcagcatc gcgccggcgg cacggtggcc
     4081 gcgctgttcg tgcgcgaagg gcagcaggtg aaggcaggcg atgtgctgat cctgctggcc
     4141 ggcgcggaag tggccgccaa cgagcaatcg ctggccgcgc aagtggtgga tctgatggcc
     4201 gagcgggccc gcctgcaggc cgaattgaac agcagcgctg cagtgatccc gccgcccgaa
     4261 tttgccaccc tgccggcaga agacaagccg ctggccgaag ccgcgctgcg ccagcagatc
     4321 caccagttcc agcagaaccg cgtggccctg acgacgcaaa agcgcgtggt cgcccagcag
     4381 gccgcccagc tgcaggaaaa ggccagcggc cttggcgagc agatgggcgc caaccgccag
     4441 gaagacagct cctatgccgc ccagctggat ggcatgcgcg aactcgccgc gcaaggctat
     4501 gccagcgtta accgcgtgcg cgagctggag cgcgcgcgcc agcagatgaa cggcgatttt
     4561 gcccgcctgt cggccgatcg cgcctccact cgcaaccaga tcggcgaaat gcgcatgcgc
     4621 agcctctcga tcgacacgga cgaccgccgc caggtggccg aagacctgcg caagacggtg
     4681 gacgagctga acgcggcgct gcccaagtgg gaagatgccc gccagcaggt ggaaaacacc
     4741 cgcatccgcg cgcctgtggg cgggcaggtg gtggggctgt cgatcttcac cgtgggcggg
     4801 gtgatcacgc cgggcgaaaa gctgatgtcc atcgtgccca gcaaggccac gctggtggtg
     4861 gaaggccatg tgcagcccac cgatgccaat gatctggtgg tgggccagaa ggcgcaggtg
     4921 cgcttccccg gcttccacga acgcagtctg ccgcagatcg agggcaccgt cacccgcgtt
     4981 tcggccgatg ccttcaccga tgatcacacc ggcaaaagct atttcaccat cgaggtgacc
     5041 gtgccgccgg cagacatggc caagctgaag ccctttgccg ccggcgggct gggcttgcgg
     5101 ccgggcttgc cagccacggt gctggtgccc ctgcggcggc gcaccttgat gggctatctg
     5161 ctggaaccgc tgaaccaggc gctgtggcgc agcggccggg aacactaacg ccccacccct
     5221 tcctcccaaa gctggcaggc gccgcaatcc ggcgcccagg atgaggcttg cccgtcatgt
     5281 tgttcggatc gctggtattt gtctttgcct ttctgccggt tgtgctgctg gcctatcacg
     5341 ccctgcgcgg cgccgggcat gaacggacgg ccaaatattt cctcgtcgcc gcctcgctgt
     5401 tcttctacgg ctggtgggat ccgcgctatg taccgctggt cagcctgtcg atcggcgcca
     5461 atttcgcgct gagcctgctg atggagcgcc agcccggccg cgccagcctg ctgctggggc
     5521 tgggcgtggc gggcaacctt gccttgctgg gctattacaa atacgcccat ttcttcgtga
     5581 cggcgctggc cggcgccacc ggcctgccct ggcatagggg ccgcatcgtg ctgccgctgg
     5641 cgatcagctt cttctccttc cagcagatcg cctatctcat cgaaagccgg cgcgatggca
     5701 aagccgcaca cagcctgctg gattatgcgc tgttcgtgct gttctttccg catctgatcg
     5761 ccgggcccat cacccatcac aaggaaatgc tgccccagtt tgccgccatg ggccgccacc
     5821 gcctgccgcc cagctatatg ccgatcggca cggcgatctt catcatgggg ctggccaaga
     5881 aggtggtggt ggccgatctc ttggcgatgc tggccaaccc cgtgttcaac gccgcgcgcg
     5941 atgggctggc gctggcacca tggcaggcgt ggcagggggc gctggcctat acgctgcagc
     6001 tctatttcga tttttccggc tattcggaca tggcgatcgg cctggggctg atgttcggca
     6061 tcctgctgcc ggtcaatttc gcctcgccct acaaatccac cagcatcgtc gaattctggc
     6121 gccgctggca tatttcgctg tcgcgcttcc tgcgcaacta tctctatttc tcgctgggcg
     6181 gcaaccgggc ggggccggtg cggcgctatg ccaatctgct ggccaccatg ctgctgggcg
     6241 ggttgtggca cggcgcgggg tggacgttta tcgcctgggg cgggctgcat ggcagctatc
     6301 tggtgatcaa ccacctgtgg cgcgggcgct ttggcccggc gcgggggcct gtggggcgat
     6361 tggccggctg gggcctcacg atgctggcgg tggtggtggc ctgggtgctc ttccgggcag
     6421 acacatttgc cagcgcggtg gccgtgctgc gggccatggc gggcgcgccg ctggcgggcg
     6481 gggcaagcgg ctatctggtg ccgcaactgc tgggctgggc agcggtggcg ctggctggcg
     6541 gggtcgcgct gtttgccccc aatgtgctgg aaatggcagg cttccccc
//
LOCUS       DASRYE010000158         7957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_9124,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000158 DASRYE010000000
VERSION     DASRYE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9124"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFF98_08355"
     CDS             complement(<1..677)
                     /locus_tag="VFF98_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63681.1"
                     /translation="MKARLAALAAMAALLPQGARADTLIDHVNGLGMDAQGQATRFTG
                     LVIGDDGKIATLLGAYDIRPRTRFAVDGHGARLLPGRLLDRGQLVRMGLSLLLGGKPL
                     ANLPPPRPEDRDLALATAQKALLAQGITTVVDMGTTIEDWQALRRAGDAGRLAIRVIC
                     YAGGIADMALIGGPGPSPWLYDGRLKLQGVWLDAGGPARARPNLIQLKNMMSRAAMDH
                     FQVAVQVA"
     gene            complement(692..1633)
                     /locus_tag="VFF98_08360"
     CDS             complement(692..1633)
                     /locus_tag="VFF98_08360"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU63682.1"
                     /translation="MPDSDPAAALPADPATPPRRIAMIGLGVMGGPIARHLARAGHGL
                     TIHARKPSRLHRWMAANPDLAVQSAASPADAAAGADVVITCVGNDDDLADVVLSPNGV
                     FSTLKRGGLFIDHTTVSARIARQIAVEARDKEILCVDAPMTGAQAGAEAGTLTLMCGG
                     RPPAIEAARPIMSAYARLIVHVGKAGTGQATKMVNQIALAGVTAGVAEALRLAQAEHL
                     DLDKVFEAISHGAAQSWQMDNRWHTMAADAFDDFGFAIDWMRKDLGLALDEGRGLGLS
                     LPITAMIDQLYADVQAMGGGRQDTSALIRRLPRRGAK"
     gene            1753..2997
                     /locus_tag="VFF98_08365"
     CDS             1753..2997
                     /locus_tag="VFF98_08365"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZU63683.1"
                     /translation="MTKPANALADSSLLSIADVHAAAQRIGGHVVRTPIHHSKTLSAI
                     CGADIWLKFENQQFTAAYKERGALNALLQLTEAQRAKGVIAASAGNHAQGLSYHGRRL
                     GVPVTIVMPKTTPTVKVMQTESVGGTVVLEGETFDEAYEHARSMEKQLGLTFVHPFDA
                     PEVAAGQGTVALEMLEDVPELDMLVIPVGGGGLASGMGTAARAIKPDIGLVGVEAELY
                     PSMYNRLKGTSYPIGGDTLAEGIAVKEPGQFTSQVLAELLDDFVLVSESELESALALL
                     LQIEKTVVEGAGAAGLAAVIAHGHLFKGRKVGIVLSGGNIDQRLLANVLLRDLARSGR
                     LARLRLTLQDRYGALFKVMEEFNRHQINIIEIWHQRIFTHLPAKGLITDIECEARDRE
                     QLEALVHALRDKGYDVSQVELN"
     gene            3241..4104
                     /locus_tag="VFF98_08370"
     CDS             3241..4104
                     /locus_tag="VFF98_08370"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZU63684.1"
                     /translation="MTVPVRFPRFYVTSPAPCPYLPERQERKVFTELKGPNAAALNDA
                     LGRIGFRRSQTVAYRPSCVDCTACVSVRVVAGEFVANGTQKRTLKRHGDLVSSICHPW
                     STTEQYDLLKRYLGARHPGGGMAAMDEMDYADMIEHTPVTSYVIEYREPGEDGRPGRL
                     VGACLTDRQADGLSMVYSFYDPGEGRPGLGNFIILDHIRRAVAEGLPYVYLGYWVDGS
                     ERMQYKVRYRPLEKLGRDGWMRMEPHEQEALINATAARRRGDGEAKGSKEGLLATDGS
                     QATAERFPVGE"
     gene            4150..6669
                     /locus_tag="VFF98_08375"
     CDS             4150..6669
                     /locus_tag="VFF98_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZU63685.1"
                     /translation="MTEAGETPKESPCRVVLLAGLVSVLGVLPGPLEGRARAEPASPA
                     PGAASSPAPAGSAGGSSPVPAATAGGSSAAPSHADGVAAGVGIAPPLVEDRLRALIPD
                     EAVAHPESWAGAALAADKASPAPEAGAAPVQANPQTTAENAPAPATPEAPPALNPASP
                     LAEPGGFTLPWPGTSVDMSALPPLEQDPALPKAMSEAVTELTAIPLDLPGATSQPAAK
                     VHRAVREPRNPGEAKQAAAKAEKRLPGGRVVLAYPDALQAFPERAAFEARFRELSSVQ
                     TPIGKDQDAAAQTAVRAASDRQLLLKLLAYFGYYDGEVNQALATPAQGEGLPQAGGGA
                     ITVRFDIQPGAHYRLGSVDLGGLADTGQDYPALRKAFAVQAGDPVSTARILAEVDHLT
                     AALGEAGYPFAKLGAPALSVDHAREQASLSLPVTPGGKYRFGAITSNLPRYLPGRHLV
                     DLARFHAGDTFRRSQMEDLHKAILATGLVSSAGIAPRETSPPKGDAPGQLALDVTMAK
                     APQHTIAGAIGYDTGEGFRLETSWEKRNIFPPEGSLRLRGVAGTNEQLAGVTVSRANF
                     HGRDRTLSLDLYADNATLTAYAARKVGTALTYQRQTTLLFQKPWSWSVGLEAEASDER
                     EGVPSGVITGRHMYITTALPLRAAFDASDDLLDPTHGGRISLRASPERSWTQGQVSNY
                     VKLQLDAAAFHPLVKGVVEALRVRIGSIIGTGIDQIAPSRRFYAGGGGSIRGFGYELV
                     GPLNSLGEPKGGLSLYEVSAETRVDTGWFGGALALVPFIDIGGVGTRSRPDFSGLRYG
                     AGLGLRYKTGFGPIRLDVGTPLDPRPGESRLGVYVSLGQSF"
     gene            6669..>7957
                     /locus_tag="VFF98_08380"
     CDS             6669..>7957
                     /locus_tag="VFF98_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63686.1"
                     /translation="MAEPAAPPPEEPPVAPRHALARWLALSGARRAVLRRRFLRALRK
                     YALGLPLALLLVVLALPLLADSPLGHRLIADLIAEMDLGRGLSAGVGRIDGSLFGHLR
                     LSEVVLRDDRGVFLRAPQLEVDWRPLALLEHRVELRNVALRRASLLRVPHLQPGSNQP
                     TDLGDWAVQVNRLAVERLTVARAVAGSERRVDLRGELEWRQARRFVSLNGRLGGGDRL
                     AALFEDDRAANRFGLDVDYVAPRGGLLAALSGARADRRLRIAGQGNWQDWHGGLVLDS
                     AGARAAALLLTHRGAHVGLAGLLGADPLVPPALRQALGGPLAITAEGDLAGKGVAGRA
                     LLASPHLLASAAGQVDSGGGLGDVTLDLASRGDMPLDDSASLSGARLHALVNRNGDGM
                     RVDWRFTAARLSAAGKGAQPLTAEGLSASGQASQSAAG"
BASE COUNT         1168 a         2690 c         2890 g         1209 t
ORIGIN      
        1 gccacctgaa cagccacctg gaaatggtcc atcgctgccc ggctcatcat gttcttcagc
       61 tggatgagat tggggcgcgc ccgcgccggg ccgccggcat ccagccacac gccctgcagc
      121 ttcagccgcc cgtcatacag ccagggcgag gggcccggcc cgccgatcag cgccatatcg
      181 gcaatgcccc cggcatagca gatcacccgg atcgccagcc gcccggcatc gcccgcccgg
      241 cgcagcgcct gccagtcctc gatggtggtg cccatgtcga caacggtggt gatgccttgc
      301 gccagcagcg ccttttgcgc cgtggccagc gccagatcac ggtcttccgg gcggggcggg
      361 ggcaggttgg ccagcggctt gccgcccagc agcagcgaca gccccatgcg caccagctgc
      421 cccctgtcga gcaggcggcc gggcaggagg cgcgcgccat ggccatccac ggcaaagcgc
      481 gtgcgcgggc ggatgtcgta ggcgcccagc agcgtggcga tcttgccatc atcgccgatg
      541 acaagccctg tgaagcgggt ggcctggccc tgggcatcca tgcccagccc gttcacatga
      601 tcgatcagcg tatcggcgcg ggcgccttgc ggcagcagcg cggccatggc ggccagcgct
      661 gccagccttg ctttcacccc gagttttgcc ttcacttggc cccccggcgc ggcaggcggc
      721 ggatcagcgc gctggtatcc tggcggccgc cgcccatcgc ctgcacatcg gcatagagct
      781 ggtcgatcat cgcggtgatc ggcagcgaga ggcccagccc gcgcccttcg tcaagcgcga
      841 ggccgagatc cttgcgcatc cagtcgatcg cgaagccgaa atcgtcgaag gcatcggcgg
      901 ccatcgtgtg ccagcggttg tccatctgcc agctttgcgc agcgccatgg gagatcgcct
      961 cgaacacctt gtcgagatcg agatgttccg cctgcgccag ccgcagcgct tcggccacgc
     1021 ccgccgttac gccggccagc gcgatctggt tcaccatctt ggtggcctgg ccggtgcctg
     1081 ccttgccgac atgaacgatc agccgcgcat aggcgctcat gatcggccgc gcggcctcta
     1141 tcgcgggggg gcggccgccg cacatcagcg tcagcgtgcc ggcctcggcg ccggcctggg
     1201 cgccggtcat cggcgcgtcc acgcacaaga tctccttgtc gcgcgcttcc acggcgatct
     1261 ggcgggcgat gcgggccgaa acggtggtgt gatcgatgaa caggccgccg cgcttcagcg
     1321 tggaaaacac gccattgggg ctgagcacga catcggccag atcatcgtca ttgccaacgc
     1381 aggtgatgac gacatcggcg ccggcggcgg catcggccgg gctggcagcg ctttgcacgg
     1441 ccagatcagg gttggccgcc atccagcggt gcaggcgcga gggcttgcgg gcgtggatcg
     1501 tcaacccgtg gccggcccgg gccagatggc gggcaatggg cccgcccatc acgccaaggc
     1561 cgatcatggc gatgcggcgg ggcggcgttg cgggatcggc gggcaaggct gctgcgggat
     1621 cggaatctgg catggcgccc ttttagcgtg tgcgggcgaa aagcaaacgg ggcatcggcg
     1681 tgtgaggcgc cgcggggcga tcttgccaaa cgccttgctc cggccacaag ggcggcctat
     1741 ccgggccgaa tcatgaccaa gccagccaac gcccttgccg attccagcct gctctccatc
     1801 gccgatgtgc acgccgccgc gcagcgcatt ggcggccatg tggtgcgcac gccgattcac
     1861 cacagcaaga cgctgagcgc catttgcggt gccgatatct ggctgaagtt cgagaaccag
     1921 cagttcaccg ccgcctacaa ggagcgcggc gcgctcaatg ccctgctgca gctgacagag
     1981 gcgcagcgcg ccaagggcgt gatcgcggcg agcgccggca atcatgcgca gggcctttcc
     2041 tatcacgggc ggcggctggg cgtgccggtc accatcgtca tgcccaagac gacccccacc
     2101 gtgaaggtga tgcagaccga aagcgtgggc ggcaccgtgg tgctggaagg cgagaccttc
     2161 gacgaggcct atgagcatgc ccgcagcatg gaaaagcagc tgggcctcac cttcgtccac
     2221 ccgttcgatg cgcccgaagt ggcggccggc cagggcacgg tggcgctgga aatgctggaa
     2281 gacgtgcccg agctggacat gctggtgatc ccggtgggcg gcgggggcct tgccagcggc
     2341 atgggcacgg cggcccgcgc gatcaagccc gatatcggcc tggtgggcgt ggaggcggag
     2401 ctctacccct cgatgtacaa ccggctgaag ggcaccagct atccgatcgg cggcgacacg
     2461 ctggccgagg gcattgccgt gaaggagccg ggccagttca cctcgcaggt gctggccgag
     2521 ctgctcgacg atttcgtgct ggtgagcgag agcgagctgg aaagcgcgct ggccctgctg
     2581 ctgcagatcg agaagacggt ggtggaaggc gcgggcgctg ccggccttgc cgcggtgatc
     2641 gcccatggcc acctgttcaa ggggcgcaag gtgggcatcg tgctgtcggg cggcaatatc
     2701 gatcagcgcc tgctggccaa tgtgctgctg cgcgatctgg cgcgctcggg ccggctggcc
     2761 cgcctgcggc tcaccctgca ggatcgctat ggcgcgctgt tcaaggtgat ggaggaattc
     2821 aaccgccacc agatcaacat catcgagatc tggcaccagc gcatcttcac ccacctgccg
     2881 gccaagggcc tgatcaccga catcgaatgc gaagcgcgtg accgcgagca gctggaagcg
     2941 ctggtgcatg ccctgcgcga caagggctat gacgtgagcc aggtggagct gaactgaggg
     3001 cgcgctagcc tcccccggcc ctctttgcca ttcccgcgcc ttttcagccc gttggccatc
     3061 atggccggcg gcatggctcg gcaacggagg cgattggccg gcggatcgcg cgtttttcgt
     3121 ggttaatagg ctttcaccag ggggcgaggc ggggcatagt aagccgaact tggaccgggc
     3181 cgcgggcatc cgctcgccgg cgcggccaag ggtctgattt gtcaaaggat catgcccgct
     3241 gtgacggttc ctgttcgctt tccccgattc tacgttacca gcccggcgcc ttgcccctat
     3301 ctgcccgagc ggcaggagcg caaggtgttc accgagctga aggggccgaa tgcggccgcg
     3361 ctgaacgatg cgctgggccg catcggcttt cgccgcagcc agacggtggc ctatcgcccc
     3421 tcctgcgtgg attgcacggc ttgcgtttcg gtgcgcgtgg tggcgggcga attcgtggcc
     3481 aacggcacgc agaagcgcac gctgaagcgc catggcgacc tggtctcctc catctgccac
     3541 ccctggtcga cgacggagca gtatgacctg ctgaagcgct atctgggcgc gcgccacccg
     3601 ggcggcggca tggcggcgat ggacgagatg gattatgccg acatgatcga gcatacgccc
     3661 gtcaccagct atgtgatcga atacagggag ccgggcgagg atggcaggcc gggccgcctg
     3721 gtgggtgcct gcctgaccga ccggcaggcc gatggcctct cgatggtcta cagcttctac
     3781 gatccgggcg aggggcggcc gggcctgggc aatttcatca tcctcgatca catccgccgg
     3841 gcggtggccg aagggctgcc ctatgtctat ctgggctatt gggtcgatgg ttcggagcgg
     3901 atgcagtaca aggtgcgcta tcgcccgctg gagaagctgg ggcgcgatgg ctggatgcgg
     3961 atggagccgc acgagcagga agcgctgatc aacgccaccg ccgcccgccg ccgcggcgat
     4021 ggcgaggcca agggcagcaa ggagggcctg ctggccacag atggcagcca ggccacggct
     4081 gagcgctttc ctgtaggcga atgacgcagt cgggttgacg gcgccgccgg ccggcggcaa
     4141 gccaaggcca tgacagaagc gggagaaacc ccgaaagaaa gcccctgccg ggtggtgctg
     4201 ctggcagggc tggtgtctgt gctgggggtg ctgcctggtc cgctggaggg ccgggcgcgc
     4261 gctgagccgg ccagtcccgc gcccggggct gccagctcgc ccgcgccggc gggcagcgcg
     4321 gggggcagct cgcccgtgcc ggcggccacg gcagggggca gcagcgccgc cccttcgcat
     4381 gccgatggcg tggcggcggg cgtgggcatt gccccgccgc tggtggaaga ccgcctgcgc
     4441 gcgctgatcc cggatgaggc ggtggcccat ccggaaagct gggctggcgc tgccctggcg
     4501 gcagacaagg ccagccccgc accggaagcc ggcgccgcgc ccgtgcaggc caatcctcag
     4561 accactgccg agaacgctcc cgcccccgcc acgccagaag cgccgccggc gctcaacccc
     4621 gcttcgccgc tggcagagcc gggcggtttc accctgccct ggcccggcac cagcgtcgac
     4681 atgtccgccc tgccgccgct ggagcaggac cccgccctgc ccaaggcgat gagcgaggcg
     4741 gtgaccgagc tgaccgccat cccgctcgat ctgcccggcg ccaccagcca gccggccgcc
     4801 aaggtgcacc gcgccgtgcg cgagccgcgc aatcccggcg aggcgaagca ggccgccgcc
     4861 aaggcggaaa agcgcttacc cggcggccgc gtggtgctgg cctatcccga tgccctgcag
     4921 gccttcccgg aacgcgcggc cttcgaggcg cgcttccgcg aattgtcgag cgtgcagacg
     4981 cccatcggca aggatcagga tgccgctgcg caaaccgctg tgcgggctgc atccgatcgg
     5041 cagttgctgc tgaaattgct ggcctatttc ggctattacg atggcgaggt gaaccaggcg
     5101 ctggccaccc ctgcgcaggg cgaggggctg ccccaggccg gcggcggggc gatcaccgtg
     5161 cgcttcgata tccagccggg tgcgcattac cggctgggct cggtcgatct gggcgggctg
     5221 gccgatacgg gccaggatta tccggcgctg cgcaaggcct ttgcggtgca ggcgggcgat
     5281 ccggtgagca cggcgcgcat cctcgccgag gtggaccatc tgaccgcggc gctgggcgag
     5341 gcgggctatc ccttcgccaa gctgggcgcg cccgcgctga gcgtggacca tgcgcgcgag
     5401 caggccagcc tcagcctgcc ggtcaccccc ggcggcaaat accgcttcgg cgccatcacc
     5461 agcaacctgc cgcgctatct gcccgggcgg catctggttg atctggcgcg cttccatgcc
     5521 ggagacacct tccgccgcag ccagatggag gatctgcaca aggcgatcct ggcgacgggg
     5581 ctggtctcca gcgccggcat cgccccgcgg gaaaccagcc cgcccaaggg cgacgcgccg
     5641 ggccagctgg cgctggacgt gaccatggcc aaggccccgc agcacaccat cgccggcgcc
     5701 atcggctatg acacgggcga gggcttccgg ctggaaacca gctgggaaaa gcgcaacatc
     5761 ttcccgcccg agggttccct gcgcctgcgc ggcgtggccg gcaccaacga acagctggcc
     5821 ggcgtcaccg tcagccgcgc caatttccac gggcgcgatc gcacgctcag ccttgatctg
     5881 tatgctgata atgccacgct cacggcctat gccgcgcgca aggtgggcac cgcgctcacc
     5941 tatcagcggc agacgacgct gttgttccag aagccgtgga gctggtctgt ggggctggag
     6001 gcggaagcct cggacgagcg cgagggcgtg cccagcgggg tgatcaccgg gcggcacatg
     6061 tatatcacca ccgccctgcc gctgcgcgcc gccttcgatg ccagcgacga cctgctcgat
     6121 cccacccatg gcgggcggat ttcgctgcgc gcctcgccgg aacgatcgtg gacgcagggg
     6181 caggtgagca attacgtgaa gctgcagctg gatgcggccg ccttccatcc gctggtgaag
     6241 ggcgtggtgg aagcgctgcg ggtgcgcatt ggcagcatca tcggcaccgg gatcgaccag
     6301 atcgcccctt cgcggcgctt ctatgccggc ggcggcggct cgatccgcgg ctttggctat
     6361 gagctggtcg gcccgctcaa ttccctgggc gagcccaagg gcggcctgtc gctctacgaa
     6421 gtctcggccg aaacgcgggt ggataccggc tggttcggcg gcgcgctggc gctggtgccg
     6481 tttatcgaca ttggcggggt gggcacgcgc tcgcggccgg atttctcggg gctgcgctat
     6541 ggcgcggggc tgggcctgcg ctacaagacc ggcttcggcc cgatccgcct cgatgtcggc
     6601 acgccgcttg atccgcggcc cggggaaagc cggctgggcg tctatgtttc gctggggcag
     6661 agcttctgat ggcagagcct gccgcacccc cgccggaaga gccgcccgtg gcgccccgcc
     6721 atgcgctggc gcgctggctg gcgctgtcgg gcgcgcggcg ggcggtgctg cgccggcgct
     6781 tcctgcgggc cttgcgcaaa tatgcgctgg gcctgccgct ggcgctgctg ctggtggtgc
     6841 tggcgctgcc gctgctggcc gacagcccgc tcggccaccg gctgatcgcc gacctgatcg
     6901 ccgagatgga tctgggccgc ggcctcagcg ccggggtggg gcggatcgat ggctcgctgt
     6961 ttggccattt gcgcctgtcc gaagtggtgc tgcgcgatga tcgcggggtg ttcctgcgcg
     7021 caccccagct ggaggtggac tggcgcccgc tggccctgct ggaacaccgg gtggagctgc
     7081 gcaatgtggc gctgcggcgg gccagcctgc tgcgcgtgcc gcatctgcag cccggcagca
     7141 accagcccac cgatctgggc gattgggccg tgcaggtgaa ccggctggcg gtggagcggc
     7201 tgacggtggc gcgcgcggtg gccggcagcg aacggcgggt ggatctgcgc ggcgagctgg
     7261 aatggcgcca ggcgcgccgc tttgtcagcc tcaatggccg gctgggcggg ggggacaggc
     7321 tggccgccct gttcgaggat gatcgcgccg ccaatcgctt cgggctggat gtggattatg
     7381 tggcgccgcg cggcgggctg ctggccgcgc tcagcggcgc ccgggcagac cggcgcctgc
     7441 gcatcgccgg ccagggcaac tggcaggatt ggcacggcgg gctggtgctg gatagcgccg
     7501 gcgcgcgcgc ggccgccctg ctgcttactc atcgcggcgc gcatgtgggg ctggccgggc
     7561 tgctgggcgc cgatccgctg gtgcccccgg cgctgcgcca ggcgctgggc ggcccgctgg
     7621 cgatcaccgc cgagggcgat ctggccggca agggcgtggc cgggcgggcg ctgctggcca
     7681 gcccccatct gctggcgagc gctgccgggc aggtggatag cggcggcggg ctgggcgatg
     7741 tcacgctcga tctggccagc cgtggcgata tgccgctgga cgatagcgcc agcctgtcgg
     7801 gcgcgcggct ccatgcgctg gttaaccgaa atggtgacgg catgcgcgtc gattggcgct
     7861 tcactgccgc gcggctgtcg gcagcgggca agggcgcgca gccgctcacg gccgaagggc
     7921 taagcgcgtc gggccaggcc agccagagcg cggcggg
//
LOCUS       DASRYE010000159         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_203595,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000159 DASRYE010000000
VERSION     DASRYE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203595"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFF98_08385"
     CDS             <1..968
                     /locus_tag="VFF98_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU63687.1"
                     /translation="AAIATSRADEVAALGADIAALPTPEALLARPDIDLAVIATPSAT
                     HAALATCALEAGKHVVVDKPVALSLAEAQRLADCARAHGRHLFAFHNRRWDSDFLSVR
                     AALAEGLPGRVTHARLTFNRFRPEVRQRWREQAGPGAGVWFDLGPHLVDQALQLFGWP
                     QAVSADIAALRTGGSADDYALVQLLYADKRVVLEAGLHAPSGLHGGPPRIAIHGTQGA
                     LVKRGLDPQEAQLVAGLRPADAAFGVDEDPLLLLDGEGGCEARAALRGCQQAFYAAVA
                     EALAQPPGTAGPNRIEEILAVQAVIEAAFTSARQGAVVALAEPAP"
     gene            1041..>2628
                     /locus_tag="VFF98_08390"
     CDS             1041..>2628
                     /locus_tag="VFF98_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZU63688.1"
                     /translation="MIRQDGKPPLAALGRLGHEGLVGLGLAGLMLPEGVAYAGIAGLP
                     PGRALAAGVAGGLIYALAGRSRFAVISPTSSSAAILAAAIASLGASGAPPAALATALV
                     AMVGLVFAGLALFRLGSLAGFVSRPVLHGFAFGLACTIIIKQWPAIVGVKSAGGPVWR
                     VAGGLIAAAPAWNIASLALGAGALAALIALRRLPQVPGALVVMAGGIALAQGADLAGH
                     GVALAGPVVLAEPVLSLPPLALVPRLAQLAAPIALILFAESWGTMRSLALPTGDRLSA
                     NRELGALGAANLASALAMGMPVGAGFSAGAANAAAGATSRWSAATAALALLALALWAG
                     PLIARIPEPVLAAVVIAALTHALSPAPFLRLWRIRRDHWIALAAAIGVLALGVLNGML
                     AAIALSIAHLLYDLAHPSISELGEVGDSRDFVDIARHPEAARVANVAIFRPNAPLIFA
                     NAEATLNAIAARAHATTAGVVVLSLEETANLDATALDALVEFAQSFAAGRRQVILARA
                     HDLVREVLLAAGRADLARNCT"
BASE COUNT          297 a          943 c          971 g          417 t
ORIGIN      
        1 tggccgccat cgccaccagc cgggccgatg aggtggcagc gctgggcgcc gatatcgccg
       61 ccctgccaac gcccgaagcg ctgctggccc ggcccgatat cgacctcgcc gtgatcgcca
      121 cccccagcgc cacccatgcc gcccttgcca cctgcgcgct ggaagcgggc aagcatgtgg
      181 tggtggacaa gccggtggcc ctctcgctgg ccgaggcgca gcggctggcc gattgcgcca
      241 gggcccatgg ccgccacctc ttcgccttcc acaaccggcg gtgggacagc gattttctct
      301 cggtgcgcgc ggccctggcc gaagggctgc cgggccgtgt cacccatgcc cgcctcactt
      361 tcaaccggtt tcgccccgag gtgcgccagc gctggcgcga gcaggccggg ccaggcgctg
      421 gcgtgtggtt cgatctgggg ccgcatctgg tcgatcaggc gctgcagctg tttggctggc
      481 cgcaagcggt cagcgccgat atcgccgccc tgcgcacagg tggcagcgcc gatgattatg
      541 ccctcgtcca gctgctttat gccgacaagc gggtggtgct ggaagcgggc ctgcatgccc
      601 cttccggcct gcatgggggg ccgccgcgca tcgccatcca cggcacgcag ggcgcgctgg
      661 tgaagcgcgg gctcgatccg caggaggcgc agctggtggc gggcttgcgc cccgccgatg
      721 ccgccttcgg ggtggacgag gacccgctgc tgctgctgga tggcgagggc ggctgcgagg
      781 cccgcgcggc cttgcgcggc tgccagcagg ccttctatgc cgccgtggcc gaggcactgg
      841 cgcagccgcc gggcaccgcc ggccccaacc ggatcgagga aatcctcgcc gtgcaggccg
      901 tgatcgaggc cgccttcacc tctgcccgcc agggcgccgt ggtggcactg gcagaaccgg
      961 cgccataagc ccaggcttgg cccggcggcc gattgccggc actgtgccag ccaaatgggg
     1021 acaggacgga aagcaggctg gtgattcggc aagatgggaa accgcccctg gcagcgctgg
     1081 gccgcctggg gcacgagggg ctggtagggc tggggctggc cgggctgatg ctgcccgagg
     1141 gcgtggccta tgccggcatt gccggcctgc cgccggggcg cgcgctggca gccggcgtgg
     1201 ccggcggatt gatctatgcg ctggccgggc gcagccgttt cgcggtgatc tcgcccacct
     1261 cctcttccgc cgcgatcctg gccgccgcga ttgccagcct tggcgccagt ggcgcgccgc
     1321 cagccgccct tgccaccgcg ctggtggcga tggtggggct ggtgtttgcc gggctggcgc
     1381 tgttccggct gggcagcctg gccggctttg tctcgcgccc ggtgctgcat ggctttgcct
     1441 ttggcctggc ctgcaccatc atcatcaagc aatggccggc gatcgtgggc gtaaaaagcg
     1501 ccggcggccc ggtgtggcgg gtggccggcg ggctgatcgc cgcggcgccg gcgtggaaca
     1561 tcgccagcct ggcgctgggg gccggcgcgc tggccgcgct gatcgcgctg cgccgcctgc
     1621 cgcaggtgcc gggcgcgctg gtggtgatgg ccggcggcat agcgctggcg caaggggcgg
     1681 atctggccgg ccatggcgtg gcgctggcag gccctgtggt gctggcagag ccggtgctca
     1741 gcctgccgcc gctggcgctg gtgccgcggc tggcccagct ggccgcgccc attgccttga
     1801 ttctctttgc cgaaagctgg ggcaccatgc gcagcctggc cctgcccacc ggcgaccgac
     1861 tgagcgccaa ccgcgagctg ggcgcgctgg gcgcggccaa cctggcctcg gcgctggcga
     1921 tgggcatgcc ggtgggggcg ggcttttccg ctggcgcggc caatgcggcg gccggcgcca
     1981 cctcgcgctg gtcggcggcc acggcggcgc tggcgctgct ggcgctggcg ctgtgggccg
     2041 gcccgctgat cgcccgcatc ccggaaccgg tgctggccgc cgtcgtcatc gccgccctca
     2101 cccatgcgct gtcgccggcg ccgttcctgc ggctgtggcg catccggcgc gaccactgga
     2161 ttgccttggc cgcggccatt ggcgtgctgg cgctgggcgt gctgaacggc atgctggccg
     2221 ccattgccct ctccatcgcc catctgctct acgatcttgc ccacccctcc atcagcgagc
     2281 tgggcgaagt gggcgacagc cgcgatttcg tcgacatcgc ccgccatccc gaagccgcgc
     2341 gcgtggccaa tgtcgccatt ttccgcccca atgcgccgct gatcttcgcc aatgccgagg
     2401 ccacgctgaa cgccattgcc gcccgcgccc atgccaccac cgccggggtg gtggtgctca
     2461 gcctggagga aaccgccaat ctggatgcca ccgcgctgga tgcgctggtg gaatttgccc
     2521 aaagctttgc cgccggccgc cgccaggtga tcctggcgcg cgcgcatgat ctggtgcgcg
     2581 aggtgctgct ggcggcgggg cgggcggatc tggcccgcaa ttgcacct
//
LOCUS       DASRYE010000160        20016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_254767,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000160 DASRYE010000000
VERSION     DASRYE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20016
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254767"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFF98_08395"
     CDS             complement(<1..334)
                     /locus_tag="VFF98_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HZU63689.1"
                     /translation="MTKALSLALGLPTLALLSGCNLAPAYHVPASAAAPAQFKADPGW
                     QVAMPADDVAKGQWWKLFGDSVLDGLEARVAVTNQNVAQYRAAYEQARGLVKQDRAAL
                     FPSVSASAG"
     gene            complement(338..3547)
                     /locus_tag="VFF98_08400"
     CDS             complement(338..3547)
                     /locus_tag="VFF98_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63690.1"
                     /translation="MNISAPFIRRPVGTLLLTLGVAMAGIAAFFNLGVAPLPKADFPF
                     VMVQANLPGASPDTMASTVASPLERHLATIAGVQELTSKSSLGSTQIAMMFDLNRDID
                     GAARDVQAAINAARADLPTTLKTNPTYRKMNPAEAPVLILALSSNKREPHEIYDAVSN
                     IVQQKLLQVDGVGNVELGGGALPSVRVEINPVQLSRAGIALEDVRTALQSASGNHPRG
                     VVEGQGGLAWQVYSNAAAVKAADFAPLIIAWRNNAPVRLSDVAQVIDAPEDVRTMGLV
                     NGRKAVPVIITRQPGANIVSTVDALKAQIPALQAELPDDIKLEVVTDRTITIRASLRD
                     VEITLLVSTLLVVVVVSLFLQSWRATMIPAAAVIVSLLGTLGVMYLSGFLLDNLSLMA
                     LTVATGFVVDDAIVVVENIARHVEEGMQPMKAALKGSREVGFTVLSISLSLIAVFTPL
                     IFMGGLVGRLFREFALTMSAAVAISLVASLTVTPMLASRVLAPETEKSGFFKVCERFF
                     NWLQRGYVRQLDWALAHRGPVLLLLAGTIVLNGFLIAVAPKGFFPTQDVGGIQGGVRA
                     DQAMSFQQLSDKFKQLVNIIQKDPAVAGEVAFSGGSRSGGGFVFVNLKPRAEREDVNA
                     VIERLRPKLARVRGVSLFLNPVQDIQAGGRQTSSSYQYVMQADNPQTLADAGDKMLRA
                     LKAKPNEVADVDIDQQDAGASAYVTVDRVAAARLGIPMYIIDDTLYDAFGQRQVANIY
                     QGLNQYHVVMEAAPSFIGKPEALENLYLPTGSGVATLSTALATAKTSNASLGSNAATG
                     SAVSTSASTLTQLASLAHWDIRSTNANVSHSDGQPSATISFNLPPGVTLSQAAQTIAT
                     TQASLKLPASVHGDFGGTAKIFQQSSSSTPMLIVAAILSIYVVLGILYESAIHPLTVL
                     STLPAAGVGAIFSLIVSGGEFDFIALVGVILLIGIVKKNAIMIIDFALDAERSEGMAP
                     LEAIREACILRFRPILMTTLAAALGAVPLAIGFGDGAELRRPLGIAIVGGLVASQILT
                     LLTTPVVYLALDKWRRRSPHEKLLARHIEHAHADQILKA"
     gene            complement(3544..6828)
                     /locus_tag="VFF98_08405"
     CDS             complement(3544..6828)
                     /locus_tag="VFF98_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63691.1"
                     /translation="MTDTPAPAPTPEATPPGGGTSNGTPPGGGSGNGTPPGGGSGNGT
                     PPGDDELIAGGGPSRPFILRPVATTLFMLAILLAGLIAWKQLPLSALPQVDYPTIQVR
                     TLYPGASPDVMALTVTAPLERQFGQMPGLARMTSNSSAGASVVTLQFGLDLQLDVAEQ
                     EVQAAINAANSLLPSDLPAPPIYAKVNPADAPILTLAVSSKTRGLGEIEGIVERQFSN
                     KIAQEQGVGLVSLSGGQRPAVRITANVQSLAAHELSLETLRTAISNANSNMAKGSFDG
                     VNKSWTIDANDQLNSAADYANLIVAWENNGPVRLSDVAKVEDSIENVRTRSWMNGTEA
                     IVIDVQRQPGANVIQTVNNIKADLPTLEAQMPADLKVSLLTDRTSGIRASVADVQFEL
                     VLAVLLVVLVIFLFLGSLRATLVAGLSVPLSLVGAFAAMWALGYSINNLTLMALTIAS
                     GFVVDDAIVVIENIARHIEAGMKPFPAALKGAQEIGFTIISLTVSLVAVLIPLLFMGD
                     VVGRLFREFAVSLAVTIVLSAVVALTMVPMLAARWLKPEHAERRFAIVDKAMHQFDRL
                     AHAYARALDWVMARARLTMLVFAGSLAITALLFWAIPKNLFPTQDTGQISAAVVGDKD
                     ISFARMAALQQQVAKAILADPAVESLSSAVGVDGQNPTLNQGRMLINLKPIEDRSGLT
                     KVLASLQAAAGGVPGAKVYFQPVQDLTIDTESGITPYRFSLQGADAALVNQWGLKLAE
                     ALRKRPELTDVNANSLERGRSVVVDINRDAAARLGLSAINIDNALYDAFGQRIISTIY
                     TQSSQNRVILSATPEMLANPASLATMYVPISSTTSVQLGSIATIRESDAPLVESRLAQ
                     FPQATIGFNVAEGVSLGAAVRAISQVEKQIGLPSALSSDFSGAASAFQSALANEAVLV
                     LAAIITVYIVLGVLYESFVHPVTILSTLPSAGIGALIALALSGYGLGVIGIIGIVLLI
                     GIVKKNAIMMIDFAVEAMRDEGMDADAAIRRAAHLRFRPIMMTTFAALFAAIPLIFGT
                     GMGYELRQPLGLAIAGGLILSQVLTLFTTPVIFILFERWAERRGFTTSPMPVAELEGP
                     EGSLGA"
     gene            complement(6825..7967)
                     /locus_tag="VFF98_08410"
     CDS             complement(6825..7967)
                     /locus_tag="VFF98_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63692.1"
                     /translation="MATALVGKYFASAGALQNAKHGRPAAAVNVVPVELADVPVTVSA
                     IGTVTPIDSATVHAQLSGNVFSVLFREGQMVKEGQVIVQIDPRPYQLALASAKASLAK
                     DEATLGTARLDLARYQKLAAQDSVARQTLDTQIATVKQDEAAVAADRAAIGTAQLNLA
                     YTAVKSPFAGRIGLKQVSVGTYATASDTNGVAVVTRTDPIDVEFSVPQAQLAAIRARA
                     GSPAGLPVTVLDQDNRTSLAQGQFSTFDNQIDTTTGTVKAKARIPNPSGPGHDGGVLF
                     PNQFVNVSMLVDTLHQVPVVPTSAVRHGSPGDFVFVVQPDKTVKLVVVKTGPSDGVKI
                     ALLEGVAKGQVVVSEGADGLADGSAVRLPGSSKNGSGQQKQHQAAQ"
     gene            complement(8250..9032)
                     /locus_tag="VFF98_08415"
     CDS             complement(8250..9032)
                     /locus_tag="VFF98_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZU63693.1"
                     /translation="MKLHGKICIVTGAASGIGLGIARRYVAEGAKVALADLNLAAAEA
                     AAAELAATGPGEAMGLAMDVTSEEQVNAGIAHVVAQWGTVDVLVSNAGIQIVNPVESF
                     AYADWKKMLAIHLDGAFLTTKACLPHMYAQGSGSIILMGSVHSKVASPLKSAYVTAKH
                     GLMGLARTIAREGGTKGVRANVVCPGFVRTPLVDRQIPQQAAELGISEEEVVKKVMLG
                     GTVDGEFTSIEDIAEVALFFAAFPTNALTGQSLVASHGWAME"
     gene            complement(9223..10674)
                     /locus_tag="VFF98_08420"
     CDS             complement(9223..10674)
                     /locus_tag="VFF98_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63694.1"
                     /translation="MKLNSSIALVAVVAALAAPVAAEAKAKPKAKAHHAAAEGLTTAE
                     ELRQAEQQLAQMQAQINTLQSKLDQTASAQAAGQAQAASDAKAAEVKADKALAAADAA
                     KVAVAKTDKAVSAVKWAGDTKVSGTVFYNFSTISQGAGASGQNKSSGTGFNLKRVYVT
                     IDHNFNSVFSARVTTDVSNVLGYTNLNVNSSTLPSSSSVVGKGLFLKHAYVQAKLDKA
                     LVIKLGSADMPWIPYVDGLSGNRYLDQGLEERDSLGNSADWGVHVSGDLGKYFSYQVS
                     LVDGGGYRNAYVTKNMDVEGRLSANVDGFYAAVGGYTGKLGKGVEETGATPLTTYRTA
                     SRLNAAAGYKGDKFTVGAEYIFAKNFLGSVTSSTDSDKTRGYSFFANYNLDKKWQVLG
                     RYDWLKFTPKATTAPTKNVYDHYFYVGLRWEPVKIVDITLLYKRDVVNNGVVKSSNGS
                     IGCTTDTTASTAVSALCGNGTYDEVGIFGQLKF"
     gene            11235..12278
                     /gene="pstS"
                     /locus_tag="VFF98_08425"
     CDS             11235..12278
                     /gene="pstS"
                     /locus_tag="VFF98_08425"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZU63695.1"
                     /translation="MFRKFAYAFGAAAVVAGVVGIAKADNFTGAGATFPAPVYSAWAQ
                     AYHGATGNELNYQAIGSGGGIRQIKAKTVDFGASDKPLQPAELNAAGLLQFPTVIGGI
                     VPIVNIPGVTAGKLRLSGPVLGDLFLGKIRRWDDAAIARLNPGVKLPSLPVTVVHRAD
                     GSGTSFIFTSYLSAVNRDWAARVGANDSVNWPTGLGGKGNDGVAAFVKQTSGSIGYVE
                     YIYARKDNVAWTYVENKAHQYPNPDPVSFAAAAANAPWGRAPGNYVLLIDEPGAKAWP
                     ISGATFLLMYKNPGNAQKAAEVLKFVDWSYKNGDAAAGKLDYVPLPLSVKNMIRKQWL
                     QITAGGKPVYVSK"
     gene            12293..13273
                     /gene="pstC"
                     /locus_tag="VFF98_08430"
     CDS             12293..13273
                     /gene="pstC"
                     /locus_tag="VFF98_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZU63696.1"
                     /translation="MAEAAIAPAGETAPQVKDLRGISRAGRNDAIFHALCLSAAMLLM
                     ATLAGLLISLIIGGWPALSHFGLKFFFSSAWDPVADDYGAAGPIVGTLITAFLSMLFA
                     LPLAIGIAVFLVEFCPKSIERWVAMAVELLAGIPSIVYGMWGLFVLGPWFNDHVILPL
                     RLAAEPGSFLEKFLGNASSGSNIFTASVILAIMVLPFIATVFRELLLSVPTYVREAAY
                     GMGCTPFEVVSNIMLPYIRKSAIGAVMLGLGRALGETMAVTFIIGNSHAFPTSIFDSG
                     STIASTIANEFAEATTDMHVSALVALGLVLFAITFTTLAVARLLLGKEKA"
     gene            13273..14148
                     /gene="pstA"
                     /locus_tag="VFF98_08435"
     CDS             13273..14148
                     /gene="pstA"
                     /locus_tag="VFF98_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZU63697.1"
                     /translation="MSAAASAGLFSRYGRRKLVNYVFVTLASMATAVAIAALVMILAS
                     LIMKGFGGINAEIFRADTPGGGGDLGTGGLRNAIVGSVMLCAMGMVIAVVIGILAGTW
                     LAEYGDGNRLAGFVRFLNDVLLSAPSILIGLFGYELVVLPMHGGSALAGAIALAILTT
                     PVVTRTTEDILRLQSNQLREAGMALGAGKGLVIRAIIWRASRTGLVTGGLLGFARMSG
                     ESAPLVFTNAGNQFFSWNVLGATASLPMTIFYFALSADENWQRLAWVAALLISVAVLS
                     ANIIGRALARGSQHK"
     gene            14184..14954
                     /gene="pstB"
                     /locus_tag="VFF98_08440"
     CDS             14184..14954
                     /gene="pstB"
                     /locus_tag="VFF98_08440"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZU63698.1"
                     /translation="MVAPPEAERVLDVKNLDFFYGKHQSLFGVDLPVARKQITALIGP
                     SGCGKSTLLRTLNRVYDLYPGQKAQGEILFNGENILAEKDLARLRARIGMVFQRPTPF
                     PMSIYDNIAFGVRLHRKVTKAEMDEIVEVSLTRAALWDEVKDKLDKSGLGLSGGQQQR
                     LCVARGIAVEPEVLLLDEPASALDPLSTAKLETALLELKDSFTIVIVTHNLQQAARIS
                     DHTAFMLLGKMIEFRPSSEAFVKPETEAMRDYVTGRYG"
     gene            complement(15087..18446)
                     /locus_tag="VFF98_08445"
     CDS             complement(15087..18446)
                     /locus_tag="VFF98_08445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU63699.1"
                     /translation="MARPVFFDPSGRRRRWTRRGLFAALIALVLLAGAFASTVVSIPS
                     PSPLPLGFERRTPLRFKAQVSNLSHELTDLFRTPPKAHGRAAAKAISVGFYTTWSDES
                     APSLVKHIGQLDWVAPTTLAIDSAGQLQVHEDNALRRIVFSSLHRPLIMPVVQNVIGD
                     VFAGAATQALFHNEGRRRAFIAALDAYLDKTGDAGVIFDFENIAPADVARYRALIAET
                     NADFDKKGRLVAVTMPVGNEDWNPRAFAQAADKIILMAYDEHWQSGTAGPIASNGWFA
                     NQVAQATRGIAPDKVIVALGSYAYDWYGGKSDSLSIEEAWLAAHDASTNVTFDRNSGN
                     SGFSYLDGNLRHDVWMLDAAASWNEMHILSQLGLGNVALWRLGSEDPGFWPALGAWRQ
                     GGTTPPDLTAIEQTANVDVEGQGEILRITSTPTTGAREVDFDARTHLATAVRYLTLPT
                     PYVVQRTGGANPKQLALTFDDGPDPKWTPQILSILEQYHVPGTFFVIGENGVANRGLL
                     EREVADGFELGNHSYTHPNMAIDTQLGINLELNTTKRLIEAYTGRSIRLFRAPYFGDA
                     EPTTADELVPADIAQQHGYTVVGLHIDTDDWKRPGVQEIINRTLAQVAAATPDRTANV
                     ILMHDGGGDRSQTVAALPAIIATLQHQGYTFVPVSTLAGISHDQVMPPLSGFDLVSVR
                     ADVFAFTALGFTIIALNWTFFFAIALGMIRAVSMSVLALLPGRPKPPLPSGEFRPKVT
                     VIIPCFNEEKVIEASVRRILESSYPYLDVIVADDGSKDGTSAVVAAAYGDNPRVKLLS
                     MPNGGKAAALNRALKEAQGEIIVALDADTQFERETIIRLVRWFIDPKIGAVAGNAKVG
                     NRVNLVTRWQAVEYTTAQNIERKALTRFEAIMVVPGAVGAWRRAALESVGGYPEDTLA
                     EDQDLTIAIQRKKWKVAYDEDAVAWTEAPESFRALAKQRFRWSFGTLQCLWKHRKIVR
                     KGKPGGLAFIGVPQAWLFQIVFACISPAIDLALVISIVGTVVRVMQHGWAQTQTDVLR
                     MAAYWMCFSAVDLTCGLIAYRLDVREKRFPAFLLLAQRFVYRQLMYWVVLKAVSSAIR
                     GVGASWGKLERTGRVSVAPQPPAPADGSPQG"
     gene            18872..19744
                     /locus_tag="VFF98_08450"
     CDS             18872..19744
                     /locus_tag="VFF98_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU63700.1"
                     /translation="MGFIHHLDGLHAVAGLLVGILVGLTGVGGGSLMTPLLVLLFGVS
                     PTTAVGTDLLYASATKTVGSAVHGWRETVDWQVMQRLALGSIPAALFTLMVLNRAGPL
                     SKATQNDLMLGLAAMLALTSVLVLLQKRIVALAGRRKLARGHRHSPRVVLGTVALGAL
                     LGTAVSLTSVGAGAIGVTALLLLYPELPMSRIVGTDIAHAVPLTLVAGMGHWIYGDVD
                     GVLLVNLLLGSVPGIIIGSLISSRSPDGVLRPLLGMVLVLSSWQLFLKASRPAEALAR
                     HALPAAAHSPATRG"
     gene            19864..>20016
                     /locus_tag="VFF98_08455"
     CDS             19864..>20016
                     /locus_tag="VFF98_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HZU63701.1"
                     /translation="MTDTLTHLARLEAESIHIIREVVAEADRPVMLYSVGKDSAVMLH
                     LARKAFY"
BASE COUNT         3297 a         6477 c         6763 g         3479 t
ORIGIN      
        1 ccccggcgct ggccgaaacg ctggggaaca gcgcggcgcg atcctgcttc accaggccgc
       61 gcgcctgttc ataggcggcg cgatactggg cgacattctg gttggtcacc gcgacgcgcg
      121 cttccagccc gtcgagcacg ctgtcgccaa acagcttcca ccattgcccc ttggccacat
      181 catcggccgg catcgccacc tgccagcccg gatcggcctt gaactgggcg ggcgcggcag
      241 cgctggccgg cacatgatag gccggcgcca gattgcagcc tgaaagcagc gccagggtgg
      301 gcaggccaag ggcgagggag agggctttcg tcatggctca ggctttcagg atctggtcgg
      361 catgggcatg ctcgatatgg cgggccagca gcttttcatg cgggctgcgg cggcgccatt
      421 tgtcgagcgc gagatagacc acaggggtgg tcagcaaggt gaggatctgg ctggcgacaa
      481 gcccgccgac gatggcaatg cccagcggcc ggcgcagctc ggcgccatcg ccaaagccga
      541 tcgccagcgg caccgcgcca agggcggcgg ccagcgtcgt catcaaaatg gggcggaagc
      601 gcaggatgca ggcctcgcgg atggcttcca gcggcgccat cccctctgaa cgttcggcat
      661 cgagggcaaa gtcgatgatc atgatcgcgt tcttcttcac gatgccgatc agcaggatga
      721 cgcccaccag cgcgatgaaa tcgaactcgc cgccgctgac gatcagcgag aagatcgccc
      781 ccacgcccgc cgccggcagg gtggacagca cggtgagcgg atggatcgcg ctttcgtaga
      841 ggatgcccag caccacatag atgctgagga tggccgccac gatcagcatc ggcgtgctgg
      901 aggaggattg ctggaagatc ttggcggtgc cgccaaaatc gccgtgcacg ctggccggca
      961 gcttcaggct ggcctgggtg gtggcgatgg tttgcgccgc ctggctcagc gttacgcccg
     1021 gcggcaggtt gaacgagatg gtggccgagg gctggccatc ggaatggctg acattggcgt
     1081 tggtgctgcg gatatcccaa tgggccaggc tggccagctg ggtcagcgtg ctggcgctgg
     1141 tggaaacggc gctgccggtg gcggcattgc tgcccagcga ggcgttgctg gtcttggcgg
     1201 tggccagcgc ggtgctcagc gtggccacgc cagagccggt gggcagatag aggttctcca
     1261 gcgcctcggg cttgccgatg aagctggggg cggcttccat caccacgtga tactggttga
     1321 gcccctgata gatattggcc acctgccgct ggccaaaggc atcatacagc gtgtcgtcga
     1381 tgatatacat ggggatgcca aggcgcgcgg cggccacccg gtccaccgtt acataggcgc
     1441 tggcgccggc atcctgctgg tcgatatcca catcggccac ctcgttgggc ttggccttca
     1501 gcgcgcgcag catcttgtcg ccggcatcgg ccagcgtttg cgggttgtcg gcctgcatca
     1561 catattgata gctgctgctg gtctgccggc cgccggcctg gatatcctgc accgggttga
     1621 gaaacaggct gaccccgcgc acccgcgcca gcttggggcg cagccgctcg atcaccgcgt
     1681 tcacatcctc gcgttcggcg cgcggcttga ggttgacgaa gacaaagccc ccgcccgagc
     1741 gcgagccgcc cgaaaaggcc acttcgcccg ccactgccgg gtctttctgg atgatgttga
     1801 cgagctgctt gaacttgtcg gacaattgct ggaacgacat cgcctggtcg gcgcgcacgc
     1861 cgccctgaat gccgccgaca tcctgcgtgg ggaagaagcc cttgggcgcc acggcgatga
     1921 ggaagccgtt cagcacaatg gtgcccgcca gcagcagcag caccggcccg cgatgggcca
     1981 gcgcccaatc cagctggcgc acatagccgc gctgcagcca gttgaagaag cgctcgcaga
     2041 ccttgaagaa gccgctcttc tcggtttccg gcgccagcac gcggctggcc agcatgggcg
     2101 tgacggtaag gctggccact aggctgatcg ccaccgccgc gctcatcgtc agggcaaatt
     2161 cgcggaacag ccggcccacc agcccgccca tgaagatcag cggggtgaac accgcgatca
     2221 ggctgagcga gatggaaagc acggtaaagc ccacctcgcg cgagcccttg agggcggcct
     2281 tcatcggctg catgccctct tccacatggc gggcgatgtt ctccaccacc acgatcgcat
     2341 catcgacgac aaagcccgtc gccaccgtca gcgccatgag gctgagattg tcgagcagga
     2401 agcccgacag atacatcacc cccagcgtgc ccagcagcga gacgatcacc gccgccgccg
     2461 ggatcatcgt ggcccgccag ctttgcagga acaggctgac caccaccacc accagcaggg
     2521 tagacaccag cagggtgatc tccacatcgc gcagcgaggc gcggatggtg atggtgcggt
     2581 cggtcaccac ttccagcttg atatcgtcgg gcagttcggc ctgcagggcg gggatctgcg
     2641 ccttcagcgc atccaccgtg gagacgatgt tggcgccggg ctggcgggtg atgatcaccg
     2701 gcaccgcctt cctgccattc accagcccca tcgtgcgcac atcctcgggc gcatcgatca
     2761 cctgcgccac atccgacagg cgcaccgggg cattgttgcg ccaggcgatg atcagcgggg
     2821 caaaatcggc cgccttcacc gcggcggcat tggaatagac ctgccaggca agcccgccct
     2881 ggccttccac cacgccgcgc gggtggttgc cgctggccga ttgcagcgcg gtgcgcacat
     2941 cttccagcgc gatccccgcg cgagacagct gcaccgggtt gatctccacc cgcaccgagg
     3001 gcagcgcgcc gccgcccagt tccacattgc ccacgccatc cacctgcagc agcttttgct
     3061 gcacgatgtt cgacacggca tcgtagatct cgtgcggctc gcgcttgttc gagctcagcg
     3121 caaggatcag caccggcgcc tcggccgggt tcatcttgcg ataggtgggg ttggtcttca
     3181 gcgtggtggg cagatcggcg cgggcggcgt tgatcgcggc ctgcacgtca cgcgcggcgc
     3241 catcgatgtc gcggttgagg tcgaacatca tggcgatctg ggtggagccg aggctggact
     3301 tggaggtcag ctcctgcacc ccggcgatgg tggccagatg ccgctccagc gggctggcca
     3361 cggtgctggc catggtgtcg gggctggcgc cgggcaggtt ggcctgcacc atcacgaagg
     3421 ggaaatccgc cttgggcagc ggcgccacgc ccaggttgaa gaaggccgcg atgccggcca
     3481 tcgccacgcc cagcgtcagc agcagcgtgc ctaccgggcg gcggatgaag ggcgccgaga
     3541 tgttcacgcg cccaggctcc cttcagggcc ttccagttcg gccaccggca tcgggctggt
     3601 ggtgaagccg cgcctttcgg cccagcgttc gaacaggatg aagatgaccg gcgtggtgaa
     3661 cagggtgagc acctggctga ggatcagccc gccggcaatc gccaggccca gcggctggcg
     3721 cagctcatag cccatgccgg tgccgaagat cagcggaatg gcggcaaaca gggcggcaaa
     3781 ggtggtcatc atgatcgggc ggaagcgcag atgggcggcc cggcggatgg cggcatcggc
     3841 atccatcccc tcgtcgcgca tggcctccac ggcaaagtcg atcatcatga tcgcgttctt
     3901 cttgacgatg ccgatcagca gcacgatgcc gatgatgccg atcaccccca gcccatagcc
     3961 cgacagcgcc agcgcgatca gcgcgccaat gccggcagaa ggcagggtgg acaggatggt
     4021 gaccggatgg acgaagcttt catacagcac gcccagcacg atatagacgg tgatgatcgc
     4081 cgccagcacc agcaccgcct cattggccag cgccgactgg aacgcggagg ccgcgcccga
     4141 gaaatcggaa ctgagcgccg agggcaggcc gatctgcttt tccacctggc tgatcgcccg
     4201 caccgccgcc cccagcgaca cgccctcggc cacgttgaag ccgatggtgg cctgggggaa
     4261 ctgggccagg cgcgattcca ccagcggcgc atcgctttcg cggatggtgg cgatggagcc
     4321 cagctgcacc gaggtggtgg agctgatcgg cacatacatg gtggcaagcg aagccggatt
     4381 ggccagcatc tcgggcgttg ccgagaggat cacgcggttc tggctggatt gggtgtagat
     4441 ggtggagatg atgcgctggc caaaggcgtc atacagcgca ttgtcgatgt tgatggccga
     4501 aaggccgagg cgcgctgccg catcgcggtt gatatccacc accaccgagc ggccgcgttc
     4561 caggctgttg gcgttcacat cggtcagctc tgggcgcttg cgcagcgcct cggccagctt
     4621 caggccccac tggttgacca gcgccgcatc cgccccctgc agcgagaagc gatagggggt
     4681 gatgccgctt tcggtgtcga tcgtgagatc ctgcaccggc tggaaataga ccttggcccc
     4741 cggcacgccg ccggccgccg cctgtaggct ggccagcacc ttggtgaggc cggagcggtc
     4801 ctcgatgggc ttgaggttga tcagcatgcg gccctggttg agcgtggggt tctgcccgtc
     4861 cacgcccacc gcagacgaca ggctctccac cgccggatcg gcgaggatgg ccttggccac
     4921 ctgctgctgc agggcggcca tgcgggcaaa ggagatgtcc ttgtcgccca ccaccgcggc
     4981 cgagatctgg ccggtatcct gggtcgggaa caggttcttg gggatcgccc agaacagcag
     5041 cgcggtgatc gccaggctgc cggcaaacac cagcatcgtc agccgcgcgc gcgccatcac
     5101 ccaatccagc gcccgggcat aggcatgggc cagccggtcg aactggtgca tcgccttgtc
     5161 aacgatggca aagcggcgtt cggcatgttc cggcttcagc cagcgggcgg ccagcatcgg
     5221 caccatcgtc agcgccacca ccgccgacag cacgatggtg acggcgaggc tgacggcgaa
     5281 ttcgcggaac aggcgcccca ccacatcgcc catgaacaac agcgggatca gcaccgccac
     5341 caggctgaca gtgaggctga tgatggtaaa gccgatctcc tgcgcgccct tcagcgcggc
     5401 ggggaagggc ttcatgccgg cctcgatatg gcgggcgatg ttctcgatca ccacgatcgc
     5461 atcatcgacg acaaagccag aggcaatcgt cagcgccatc agcgtgaggt tgttgatcga
     5521 atagcccagc gcccacatgg cggcaaaggc ccccaccagg ctgagtggca ccgaaaggcc
     5581 ggccaccagc gttgcccgca ggctgcccag gaacaggaag atcaccagca ccaccagcag
     5641 caccgccagc accagttcaa actgcacatc ggccaccgag gcgcggatgc cgctggtgcg
     5701 gtcggtcagc aggctgacct tgagatcggc cggcatctgg gcttccagcg tcggcaggtc
     5761 ggccttgatg ttgttcaccg tctggatgac attggcgccg ggctggcgct gcacgtcgat
     5821 cacgatcgcc tcggtgccgt tcatccacga acgggtgcgc acgttctcga tcgaatcttc
     5881 caccttcgcc acatcggaca ggcgcaccgg cccgttgttc tcccaggcga cgatcagatt
     5941 ggcataatcg gccgcgctgt tcagctggtc gttggcatcg atcgtccagc ttttgttaac
     6001 gccatcgaag ctgcccttgg ccatgttcga attggcattg gaaatggccg tgcgcagggt
     6061 ttccaggctc agctcgtggg cggccagcga ttgcacattg gcggtgatgc gcaccgccgg
     6121 ccgctgcccg cccgacagcg agacaaggcc cacgccctgt tcctgcgcga tcttgttcga
     6181 gaactgccgc tcgacgatcc cttcgatctc gcccaggccg cgcgtcttcg agctgaccgc
     6241 cagggtgagg atcggcgcgt cggcggggtt caccttggca tagatggggg gggcgggcag
     6301 gtctgatggc agcagcgaat tggccgcgtt gatggctgcc tgcacttcct gctcggccac
     6361 atccagctgc aggtcgaggc cgaactgcag ggtgacgaca gaggcgcccg ccgaggaatt
     6421 ggaagtcatg cgggcaaggc cgggcatctg gccgaactgg cgctccagcg gggcggtcac
     6481 ggtcagcgcc atcacatccg ggctggcgcc gggatagagc gtgcgcacct ggatggtggg
     6541 ataatccacc tgcggcaggg cggaaagcgg cagctgcttc caggcgatca ggcctgccag
     6601 caggatcgcc agcatgaaca gcgtggtggc caccggccgc aggatgaacg gccgcgaggg
     6661 gccgccgccg gcaatcagct cgtcatcgcc cggcggcgtt ccattgccgg agccgccgcc
     6721 cggcggcgtt ccattgccgg agccgccacc cggcggcgtt ccattgctag tgccgccgcc
     6781 cggcggcgtg gcctcgggcg tgggggcagg ggctggcgtg tcggtcattg ggcggcctga
     6841 tgctgctttt gctggccact gccattcttg ctactgcccg gcaggcgcac ggcagagcca
     6901 tcggccagcc cgtcggcgcc ctcgctcacc accacctggc ccttggccac gccctccagc
     6961 agggcgatct tcacgccatc gctggggccg gttttcacca ccaccagctt caccgtcttg
     7021 tcgggctgca ccacgaagac gaaatcgccc ggcgagccat ggcgcaccgc gctggtgggc
     7081 accaccggca cctgatgcag cgtgtcgacc agcatggaca cgttgacgaa ctggttgggg
     7141 aacaacacgc cgccatcatg gcccggcccg gatgggttgg ggatgcgcgc cttggccttc
     7201 accgtgccgg tggtggtgtc gatctggttg tcgaaggtgg agaattggcc ctgggcaagg
     7261 ctcgtccggt tatcctggtc gagcacggtg acaggcaggc ccgccggcga gccggcccgc
     7321 gcgcggatgg cggccaactg cgcctgcggc acggaaaatt cgacatcgat ggggtctgtg
     7381 cgggtgacaa cggcgacgcc attggtgtcg ctggcggtgg cataggtgcc aacgctcacc
     7441 tgcttcaggc caatgcgccc ggcaaagggc gatttcaccg ccgtgtaggc caggttcagc
     7501 tgggcggtgc cgatggcggc ccggtcggcg gcaacggcgg cctcgtcctg cttcacggtg
     7561 gcgatctggg tgtccagcgt ctggcgggcc accgaatcct gtgcggccag cttctgatag
     7621 cgggccagat ccagccgggc ggtgcccagc gtggcctcat ccttggcgag gcttgccttg
     7681 gccgaggcca gtgccagctg ataggggcgc gggtcgatct gcacgatcac ctggccttcc
     7741 ttcaccatct gcccctcgcg gaacagcacg gaaaacacat tgccggacag ctgggcgtgc
     7801 acggtcgcgc tgtcgatcgg cgtgaccgtg ccgatggcgc tgacggtgac cggcacatcg
     7861 gccagctcca ccggcacgac attgacagcg gccgccggcc ggccatgctt ggcattctgc
     7921 aaggcgccgg ccgaggcgaa atatttgccg accagcgcag tggccaccag caccgtgcca
     7981 acggcggtgt aaaccgccca ggagggccac ctgcgccggc cgttctcgtc ctcgtcgtgg
     8041 cgatcggggg aggcgaagct gctcagggga actctcctta agacctttgt gcggggctgg
     8101 cggcttgtgc cgcacagctc ccatgacgag catgcctttg cgcgagggga gccggcaaag
     8161 gcaatgctca aacggtttca atctgtatcg aggcgtaaca gcgcgatggc gacaggcgcg
     8221 tgacgggcgc ctgaaagcgg gtttttcgct tattccatcg cccagccatg gctggccacc
     8281 agcgactggc cggtgagcgc attggtgggg aaggcggcaa agaacagcgc cacctcggcg
     8341 atatcctcga tactggtgaa ctcgccatcc accgtgccgc ccagcatcac cttcttcacc
     8401 acctcctcct cggaaatgcc cagttcggcc gcctgctggg ggatctgccg atcaaccagc
     8461 ggggtgcgca caaagcccgg gcacaccaca ttggcgcgca cgcccttggt gccgccctcg
     8521 cgcgcgatgg tgcgggccag gcccatcagc ccgtgcttgg ccgtgacata ggccgacttc
     8581 agcgggctgg cgaccttgga atgcaccgag cccatgagga tgatgctgcc cgagccttgc
     8641 gcatacatgt gcggcaggca ggccttggtg gtgaggaagg cgccatcgag atggatcgcc
     8701 agcatctttt tccagtcggc ataggcgaag gattcgaccg ggttgacgat ctggatgccg
     8761 gcgttcgaca ccagcacatc caccgtgccc cattgcgcca ccacatgggc gatgccggcg
     8821 ttcacctgct cctcgctggt cacatccatg gccaggccca tggcctcgcc ggggccggtg
     8881 gcggcaagct ctgccgcggc ggcttcggcg gcggccaggt tcagatcggc cagcgccact
     8941 ttcgcgcctt cggccacata gcgccgggca atgccaaggc caatgccgct ggccgcgcca
     9001 gtgacgatgc agatcttgcc gtgaagcttc atgagggagc tctccgctgg gagggaagga
     9061 aaggggaagg gcggggcgca ggttgtcctg tcgctgcggc gcgaatgtgg acgaaatcgg
     9121 cgggctgggg aaggggggca ggtgcgcacc ccggcaaggg gggcgcaaaa agggggcagg
     9181 ccagccggcc cgccccccat tcgctgtcag gctctgaaag gatcagaact tgagctggcc
     9241 gaagataccc acttcgtcgt aggtgccatt gccgcacagg gccgagacgg cggtgctggc
     9301 agtggtgtcg gtggtgcagc cgatcgagcc gttcgagctc ttgaccacgc cgttgttcac
     9361 cacgtcacgc ttgtagagca gggtgatgtc gacgatcttg accggctccc aacgcagacc
     9421 cacgtagaag tagtggtcat agacgttctt ggtgggggcg gtggtcgcct tgggggtgaa
     9481 cttcagccag tcgtaacggc ccagaacctg ccacttcttg tcgaggttgt agttggcgaa
     9541 gaacgagtag ccgcgagtct tgtcgctgtc ggtcgaggac gtgaccgagc cgaggaagtt
     9601 cttggcgaag atgtattcgg cgccaacggt gaacttgtcg cccttgtagc cggcggcggc
     9661 gttcaggcgc gaggcggtgc gataggtggt gagcggggtg gcgccggttt cttccacgcc
     9721 cttgcccagc ttgccggtgt agccacccac ggcggcatag aagccatcga cgttggccga
     9781 caggcggcct tccacgtcca tgttcttggt cacgtaggcg ttgcgatagc cgccgccgtc
     9841 gaccagcgag acctggtagg agaagtactt gccaaggtca cccgagacgt ggacacccca
     9901 gtcggccgag ttgccgaggc tgtcgcgttc ttccaggccc tggtcgaggt agcggttgcc
     9961 cgacagacca tcgacatagg ggatccaggg catgtcggcc gaacccagct tgatgaccag
    10021 cgccttgtcc agcttcgcct gcacataggc atgcttcagg aacaggccct tgccgacaac
    10081 gctgctgctc gagggcaggg tcgagctgtt gacgttcagg ttggtgtaac ccagcacgtt
    10141 cgacacgtcg gtggtgacgc gggccgagaa caccgagttg aagttatgat cgatcgtcac
    10201 atagacgcgc ttcaggttga agccggtgcc cgagctcttg ttctggcccg aggcgccagc
    10261 gccctgcgag atggtgctga agttgtagaa cacggtgccg ctgaccttgg tgtcgccagc
    10321 ccacttcacg gcgctcacgg ccttgtcggt cttggccacg gccaccttgg cggcgtcggc
    10381 ggcggccagg gccttgtcgg ccttcacttc ggcggccttg gcatcgctgg cggcctgggc
    10441 ctggccggcg gcctgggccg aggcggtctg gtccagcttg gactgcagcg tgttgatctg
    10501 ggcctgcatc tgggccagct gctgctcggc ctggcgcagt tcttcagcgg tggtcaggcc
    10561 ttcggcggcg gcgtgatggg ccttggcctt gggcttcgcc ttggcttcgg cggccacggg
    10621 cgcggccagc gccgccacca ccgcgaccag cgcgatcgaa gaattcagtt tcatacaatt
    10681 gctccctaaa tgcccgcccc ccgttgcgaa aaacagggcg agcgaaacct cgataaaaaa
    10741 cacacacacg cagccgggcc cgcggcagga accaggccgc gcaggcacaa gagtgccccc
    10801 gcgcccaagc gccccacgtc aaacccggtt tgccggcctt caatgcccgg ctgcggccca
    10861 atctagagtg ttgcaacccg cgcctgcgag gcgttgtcgc ctacgcatta cgcgggtcga
    10921 agtggcaaca aacaaagggt tatcaaagag aagcccttgc cagcgccaac tctgggcaga
    10981 cccaaaccag cgattgccgg ttaggtagcg attgttacat tcctgtgtaa cgcagcccgc
    11041 cgcaagaggg tgcgcgcgtt ctttcgccgg ctgttgcaat tgcgcaacga tgtgtgagac
    11101 ataggatcgg gtctgccagg cggttgtctc ggggttggcc ctggggtggc gggaaggttt
    11161 ttgtcacaaa gtcgtcatgt gcggggggta gcgccgctgg gcctaccctc ggttcgcagt
    11221 ttggagattc tctcatgttc cggaaattcg cgtatgcgtt tggcgccgcg gccgttgttg
    11281 ccggcgtcgt cggtatcgcc aaggccgaca atttcaccgg cgctggcgcc accttccccg
    11341 cgcccgtcta ttcggcatgg gcccaggcct atcatggcgc caccggcaat gagctgaact
    11401 atcaggccat cggttcgggc ggcggtatcc gccagatcaa ggccaagacg gtcgacttcg
    11461 gcgcttcgga caagccgctg cagcccgccg agctgaatgc cgccggcctg ctgcagttcc
    11521 ccaccgtgat cggcggcatc gtgccgatcg tcaacatccc gggcgtcact gccggcaagc
    11581 tgcgcctttc gggcccggtg ctgggcgatc tgttcctcgg caagatccgc cgctgggatg
    11641 atgccgccat cgcccgcctc aaccccggcg tgaagctgcc ctcgctgccg gtgaccgtgg
    11701 tgcaccgcgc cgacggttcg ggcacctcgt tcatcttcac ttcctacctg tcggccgtga
    11761 accgcgactg ggctgcccgc gtgggcgcca atgattcggt gaactggccc accggccttg
    11821 gcggcaaggg caacgatggc gtcgccgctt tcgtgaagca gacctcgggt tcgatcggct
    11881 acgtggaata tatctacgcc cgcaaggaca acgtggcctg gacctatgtc gagaacaagg
    11941 cccaccagta tccgaacccg gatccggtgt cgttcgccgc ggctgccgcc aacgctccct
    12001 ggggccgcgc tcccggcaac tatgtgctgc tgatcgacga gcccggcgcc aaggcctggc
    12061 cgatctcggg tgcgaccttc ctgctgatgt acaagaaccc cggcaacgcc cagaaggccg
    12121 ccgaagttct gaagttcgtc gactggtcgt acaagaacgg cgatgcggcc gccggcaagc
    12181 tggactatgt gccgctgccg ctgtccgtga agaacatgat ccgcaagcag tggctgcaga
    12241 tcaccgcggg cggcaagcct gtgtatgtga gcaagtaaga ctggatagaa ccatggctga
    12301 agccgcgata gctccagccg gcgaaaccgc cccgcaggtc aaggacctgc ggggcatttc
    12361 ccgcgccgga cgtaacgatg ccatcttcca cgcgctgtgt ctgtcggcgg ccatgctgct
    12421 gatggccacg cttgccggcc tgttgatctc gctgatcatc ggcggctggc cggcgctttc
    12481 gcacttcggc ctgaagttct tcttctccag cgcctgggat ccggtggccg atgactatgg
    12541 cgctgccggc cccattgtcg gcacgctgat caccgctttt ctctcgatgc tgtttgccct
    12601 gccgctggcg atcggcattg cggtgttcct ggtggaattc tgccccaagt cgatcgagcg
    12661 ctgggtggcg atggcggtgg aactgctcgc gggcattcct tccatcgtct atggcatgtg
    12721 gggcctgttc gtgctggggc cgtggtttaa cgaccatgtg atcctgccgc tgcgcctggc
    12781 tgccgaaccc ggctcgttcc tcgagaagtt cctcggcaat gcctcgagcg gctcgaacat
    12841 cttcaccgcc tcggtgatcc tggcgatcat ggtgctgccc ttcattgcca ccgtgttccg
    12901 cgaactgctg ctctctgtgc ccacctatgt gcgcgaggcg gcctatggca tgggctgcac
    12961 ccccttcgag gtggtcagca acatcatgct gccctatatc cgcaagagcg ccatcggcgc
    13021 tgtcatgctg ggcctgggcc gcgccctggg cgagacgatg gccgttacct tcatcatcgg
    13081 caacagccac gccttcccca ccagcatttt cgacagtggc agcaccatcg cttccaccat
    13141 cgccaacgaa tttgccgagg caacgacgga catgcacgtc tcggcgctgg tggcgctggg
    13201 tctggtgctg tttgccatca cgttcaccac gctggcggtg gcccgcctgc tgctgggcaa
    13261 ggagaaggcc tgatgagcgc cgctgcttct gccggcctgt tctcgcgcta tgggcgccgc
    13321 aagctggtta actatgtgtt tgtcacgctg gccagcatgg ccacggccgt ggcgattgcc
    13381 gcgctggtga tgatcctcgc ctcgctgatc atgaagggct ttggcggcat caacgccgag
    13441 atcttccgcg ccgacacgcc cggcggcggc ggcgatctgg gcacgggcgg cctgcgcaat
    13501 gccatcgtcg gctcggtgat gctgtgcgcc atgggcatgg tcatcgcggt ggtcatcggc
    13561 attctggcgg gcacctggct ggccgaatat ggcgatggca acaggctggc cggcttcgtg
    13621 cgcttcctca acgacgtgct gctctcggcg ccctccatcc ttatcggcct gttcggctat
    13681 gagctggtgg tgctgccgat gcatggcggc tcggcgctgg ccggcgccat cgcgctggcg
    13741 atcctgacga cgcctgtcgt cacccgcacc accgaagaca tcctgcgcct gcaatcgaac
    13801 cagctgcgcg aggccggcat ggcgctgggc gccggcaagg gcctggtgat ccgcgcgatc
    13861 atctggcgtg cctcgcgcac ggggctggtc accggcggcc tgctcggctt tgcccgcatg
    13921 agcggcgaat ccgcgccgct ggtgttcacc aatgccggca accagttctt cagctggaac
    13981 gtgctgggcg ccaccgcctc gctgccgatg acgatcttct acttcgccct cagcgccgat
    14041 gagaactggc agcgcctggc ctgggtggcc gcgttgctga tctctgtcgc cgttctttcc
    14101 gccaacatca tcgggcgcgc ccttgcgcgc gggagccagc acaagtgaac ttcaacgctt
    14161 cttcggccga tacggccacc gtcatggttg cgccgcccga ggcagagcgc gtgctcgacg
    14221 tgaagaacct cgatttcttc tacggcaagc accagtcgct gttcggcgtc gatctgccgg
    14281 tggcgcgcaa gcagatcacc gcgctgatcg gcccctcggg ctgcggcaaa tccaccctgc
    14341 tgcgcacgct gaaccgggtc tatgatctct atcccggcca gaaggcccag ggcgagatcc
    14401 tcttcaacgg cgagaacatc cttgccgaga aggatctggc ccggctgcgc gcgcgcatcg
    14461 gcatggtgtt ccagcgcccg acgccgttcc ccatgtcgat ctacgacaac attgcctttg
    14521 gcgtgcgcct gcaccgcaag gtgaccaagg cggagatgga cgagatcgtc gaggtttcgc
    14581 tcacccgcgc ggcgctgtgg gacgaggtga aggacaagct cgacaaatcg ggcctgggcc
    14641 tttccggcgg ccagcagcag cgcctgtgcg tggcgcgcgg cattgccgtg gaacccgaag
    14701 tgctgctgct cgacgagccg gcctcggcgc tggacccgct gtccaccgcc aagctggaaa
    14761 ccgcgcttct ggagctgaag gacagcttca ccatcgttat cgtcacccat aacctgcagc
    14821 aggccgcccg catcagcgat cacacggcct tcatgctgct gggcaagatg atcgagttcc
    14881 gcccctcctc ggaagctttc gtgaagcccg agacagaggc catgcgcgat tatgtgaccg
    14941 gccgctacgg ctgatcgcgc cttaccgcat tcacataaaa aaccgccggc aggagcgttc
    15001 ctgccggcgg ttttttatgt gcccgggatc gggcccgcgg ccggcaggcc gcaaggaggg
    15061 atgccccctg cggcccgccg gccctgtcag ccttgcgggc tgccgtctgc cggggcgggg
    15121 ggctgggggg ccacgctcac ccggccggtg cgctccagct tgccccagct ggcgcccacg
    15181 ccgcggatcg cgctgctgac cgccttcagc accacccaat acatcagctg gcgatagacg
    15241 aagcgctgcg ccagcagcag gaaggcgggg aagcgcttct cgcgcacatc gagccggtag
    15301 gcgatgaggc cgcaggtgag atcgaccgcg ctgaagcaca tccagtaggc ggccatgcgc
    15361 agcacgtcgg tctgggtttg cgcccagcca tgctgcatca cccgcaccac cgtgccgacg
    15421 atcgagatga ccagcgccag atcgatcgcc ggcgagatgc aggcaaagac gatctggaac
    15481 agccaggcct gcggcacgcc gatgaaggcc agcccgcccg gcttgccctt gcgcacgatc
    15541 ttgcggtgct tccacaggca ttgcagcgtg ccgaaagacc agcggaagcg ctgcttggcc
    15601 agcgcccgga agctttcggg cgcctcggtc caggccacgg cgtcctcgtc gtaggccacc
    15661 ttccacttct tgcgctggat ggcgatggtg aggtcctggt cctcggccag cgtgtcttcg
    15721 ggatagccgc ccacgctttc cagcgccgcg cgccgccagg cgcccaccgc gcccggcacc
    15781 accatgatcg cctcgaagcg ggtgagggcc ttgcgctcga tgttctgcgc cgtggtatat
    15841 tccaccgcct gccagcgggt gacaaggttc acccggttgc ccaccttggc attgcctgcc
    15901 accgcgccga tcttggggtc gatgaaccag cgcacgaggc ggatgatcgt ctcgcgctcg
    15961 aactgggtgt cggcatcgag ggcgacgatg atctcgccct gggcttcctt cagcgcccgg
    16021 ttcagcgccg ccgccttgcc gccattgggc atggacagca gcttcacccg cggattgtcg
    16081 ccataggcag cggcgaccac cgcgctggtg ccatccttgg agccatcatc ggcaacgatc
    16141 acgtcgagat agggatagga ggattccagg atccgcctca ccgaggcctc gatcaccttc
    16201 tcctcgttga agcaggggat gatcaccgtc accttggggc ggaactcgcc gctgggcagc
    16261 ggcggcttgg gccggcccgg cagcagcgcc agcaccgaca tcgacaccgc ccggatcatc
    16321 cccagcgcga tggcgaagaa gaaggtccag ttgagagcga tgatggtgaa gccgagcgct
    16381 gtgaaggcga acacatcggc gcgcaccgag acgagatcga agcccgagag cggcggcatc
    16441 acctgatcgt ggctgatgcc ggcaagggtg gagacgggca cgaaagtgta gccctggtgc
    16501 tgcagcgtgg cgatgatggc gggcagggcg gccaccgttt gcgagcggtc gccgccgcca
    16561 tcatgcatca ggatcacatt ggcggtgcga tcgggggtgg ccgcggccac ctgcgccagc
    16621 gtgcggttga tgatctcctg cacgcccggg cgcttccagt cgtcggtgtc gatatgcagg
    16681 cccaccacgg tgtagccgtg ctgctgggcg atgtcggccg gcaccagctc gtcggcggtg
    16741 gtgggctcgg cgtcgccgaa atagggggcg cggaacaagc ggatcgagcg gccggtatag
    16801 gcctcgatca gacgcttggt ggtgttcagt tcaaggttga tgcccagctg ggtgtcgatc
    16861 gccatgttgg ggtgggtgta gctgtggttg cccagctcga aaccgtcggc cacctcgcgt
    16921 tcgagcaggc cccggttggc cacgccattc tcgccgatga cgaagaaggt gcccggcaca
    16981 tggtattgct cgaggatgga gaggatctgc ggtgtccact tggggtctgg cccgtcgtcg
    17041 aaggtgagcg ccagctgctt gggattggcg ccgccggtgc gctgcaccac atagggcgtg
    17101 ggcagggtga gatagcgcac agcggtggcc agatgcgtgc gggcatcaaa atccacctcg
    17161 cgcgcgccgg tggtgggcgt ggaggtgatg cgcaggatct cgccctggcc ctccacgtcg
    17221 acattggcgg tctgctcgat ggcggtgaga tcgggcgggg tggtgccgcc ctgccgccag
    17281 gcccccagcg ccggccagaa gcccggatct tccgaaccca gccgccacag cgccacattg
    17341 cccagcccca gctgcgagag gatgtgcatc tcgttccagc tggcggcggc atccagcatc
    17401 cacacatcgt ggcgcagatt gccatccaga taggaaaagc cgctgttgcc gctgttgcga
    17461 tcgaaggtga cgttggtgga ggcatcatgg gcggccagcc aggcctcttc gatgctgagg
    17521 ctgtccgatt tgccgccata ccagtcgtag gcataggagc cgagggcgac gatcaccttg
    17581 tcgggggcga tgccgcgggt ggcctgcgcc acctggttgg caaaccagcc gttggaagca
    17641 atggggccgg cggtgccgct ctgccagtgc tcgtcatagg ccatcaggat gatcttgtcg
    17701 gccgcctggg cgaaggcgcg cgggttccag tcctcgttgc ccaccggcat ggtaacggcg
    17761 accagccggc ccttcttgtc gaaatcggcg ttggtctcgg cgatcagcgc gcgatagcgg
    17821 gcgacatcgg ccggcgcgat attctcgaag tcgaagatga cgccggcatc gccggtcttg
    17881 tcgagatagg catcgagggc ggcgatgaag gcgcgccggc gcccctcgtt gtggaacagg
    17941 gcctgggtgg cggcgccggc aaacacatcg ccgatcacat tctgcaccac cggcatgatc
    18001 agcggccggt gcaggctgga aaagacgatg cggcgcaggg cgttgtcctc gtgcacctgc
    18061 agctggccgg cgctgtcgat ggcaagggtg gtgggcgcca cccagtccag ctggccgata
    18121 tgcttcacca gcgagggcgc gctctcgtcc gaccaggtgg tgtagaagcc caccgagatc
    18181 gccttggcgg ccgcccggcc atgggccttg ggcggcgtgc ggaacaggtc ggtcaactcg
    18241 tgcgagagat tgctgacctg ggccttgaag cgcagcggcg tgcgccgttc aaagcccagc
    18301 ggcagcggcg agggagaggg gatggagact acggtggacg caaacgcccc cgccagcagc
    18361 accagcgcga tgagcgccgc gaacaggccg cggcgcgtcc agcgccggcg gcggcccgat
    18421 ggatcgaaga agacaggcct tgccacgttt cgaaataccg tttccgttta aaggatgcgg
    18481 ggggcatgcc ggcagcgcgc caacaggccg tgcctgcctt gcgctgctgt tccccaccga
    18541 tgcaaggcga tagcattggc aggcgggccg gggaaccgaa atcgcccgcc cggccgcctg
    18601 ccgatgctgc gctgcgccaa aagcgggcct atcctgcctt cgcggcacag cagaatttct
    18661 tgtgtgccgg gcgggcgcgc gggcaaactc aagcttgccc gtagacttgc cctgcctcta
    18721 ggaggcggcg cagacaggcg cgatctgtgc ccgctgtgcc gcatggccgc tgtttcggca
    18781 gcgatggcgt ggcaggcgtg gcagcgatgc gccgagtgtg gcaggatctg gcggcgagcc
    18841 cacgcgggca gccgggaaag gatagcgaaa gatggggttc atccatcacc tggatggctt
    18901 gcatgcggtg gccggattgc tggtgggcat tctggtgggc ctgacagggg tgggcggcgg
    18961 atcgctgatg acgccgctgc tggtgctgct gttcggcgtc agcccgacaa cggcggtggg
    19021 caccgacctg ctctatgcct cggccaccaa aacggttggc tcggccgtgc atggctggcg
    19081 cgaaacggtg gactggcaag tgatgcagcg cctggcgctg ggcagcattc cggccgcgct
    19141 gttcacgctg atggtgctga accgcgccgg gcccctgtcg aaggccacgc agaacgatct
    19201 gatgctgggg ctggcggcga tgctggcgct cacctcggtg ctggtgctgc tgcaaaagcg
    19261 cattgtggcg ctggccgggc ggcgcaagct ggcccggggc catcgccaca gccccagggt
    19321 ggtgctgggc acggtggcgc tgggggcgct gctgggcacg gcggtgtcgc tcacctcggt
    19381 gggggccggc gcgatcggcg tgaccgcgct gctgctgctt tatcccgaac tgccgatgtc
    19441 gcgcattgtc ggcaccgata tcgcccatgc cgtgccgctg acgctggtgg cgggcatggg
    19501 gcactggatt tatggcgatg tcgatggcgt gctgctggtg aacctgctgc tgggctcggt
    19561 gccgggcatc atcatcggca gcctcatttc cagccgctcg cccgatgggg tgctgcgccc
    19621 gctgctgggg atggtgctgg tgctctcgtc ctggcagctg ttcctcaagg cctccaggcc
    19681 ggccgaggca ctggcgcgcc acgccctgcc ggccgccgcg cacagccccg ccacgcgcgg
    19741 ctaggcctca cccagccagg ctggcgcccc cttctttcat gggggcaagt ttcacttgtt
    19801 tgcgccgccc cttgcgatgc ggcagggggc gcactcacga atttcagcgg atatcccacg
    19861 gccatgaccg atacactcac tcatttggcg cgtcttgagg cggagagtat acatattata
    19921 cgggaggttg tggcggaggc agatcgtcct gtgatgctgt attcggtggg caaggattcg
    19981 gcggtgatgc tgcatctggc gcgcaaggcg ttttat
//
LOCUS       DASRYE010000161         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_181902,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000161 DASRYE010000000
VERSION     DASRYE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181902"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFF98_08460"
     CDS             complement(<1..283)
                     /locus_tag="VFF98_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63702.1"
                     /translation="MLHTMLDYAALAVVSAVIREGTFERAAAALCISPSAVSQRVRAL
                     EERLGASLIARTQPCQPTDLGRRLCAHFDRVRLLEAELAPTLPAGMARRP"
     gene            346..963
                     /locus_tag="VFF98_08465"
     CDS             346..963
                     /locus_tag="VFF98_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZU63703.1"
                     /translation="MTLLFPPFLAGFALCAALIVAIGAQNLFVLRQGLLRAHVGPIVL
                     FCIAADVALIGAGVGGVAAALRALPQLTLGLTLGGAAFLAWYGVQALQRMAAGEAVTV
                     AAGQAQPLAGALAAVAGFTFLNPHVYLDTMLLMGAAGAAWPAPLRPLFVAGAGSASAL
                     WFVALGYGARLLAPVFARPAAWRVLDGLVGATMLALAASLALRAL"
     gene            complement(971..2497)
                     /locus_tag="VFF98_08470"
     CDS             complement(971..2497)
                     /locus_tag="VFF98_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU63704.1"
                     /translation="MVAHTKPAQITGATLLLAGAVLALSNFMVVLDTTIANVSVAHIA
                     GALGISSTQGTWVITSYSVAEAICVPLTGWLAGRFGTVRTFMFGMVGFGVFSVLCGLS
                     TSLAMIVLCRVGQGLCGGPLMPLSQTLMLRIFPREQHGQAMGLWAMTTVVAPILGPIL
                     GGTISDNWSWNWIFFINIPVAALCVFGTYRLLRPAETELADLRVDKMGLAMLVVWIGA
                     LQIMLDLGREHDWFGSSLITCLAVIAGVVVLFFIAWEITDRAPIVDLRIFRYRGFTVS
                     VLSLSFAFAAFFASAVIIPQWLQTSMNYTATKAGYVTAFSGVCAVIMSPIVAKLLDKV
                     DPRMLVFIGILWMGFTAFLRTLWSSDVDFWTMAMPQLLQGAGMPFFFIPLTTIGLGAV
                     RPEETASAAGIMSFMRTMAGAIGTSVSTTLYEDGATTARSEIVSKLNSQPTIDALSSS
                     GMPFEQIRGTIAQSVTTQADMISFTNLFVLSSLIFVVSALIIWLAPRPGHAVDTSAAH
                     "
     gene            complement(2736..3917)
                     /locus_tag="VFF98_08475"
     CDS             complement(2736..3917)
                     /locus_tag="VFF98_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZU63705.1"
                     /translation="MAEADPTALLDAPKGDPEIIESIAEARRQAARRRLLSGIAGGIA
                     LAALAAGGWYALVGSHHITTDNAYVGADTAQITPMVEGQVAEVEVRDTQAVHKGDILV
                     RIDPRDAQIALAQAEADLAKARRQYGQTSFTSAALDAQTKARAAAIASAQAQLVSAQA
                     AYDKANIDLARRRKLAPSGAVSGDELTSAVNAFAAARANLELARAGVAQASASHGAAQ
                     GDFAATNALIAGTTMATSPDVLAAQAKVDKAKLDLERTLIRAPVDGIVTSRAVQVGQR
                     VAAGATLMRVVPVNALYVDANYKEGQLDKVRPGQSVELTSDLYGSGVVFHGKVQGFSG
                     GTGSAFALIPAQNATGNWIKVVQRLPVRVALDPRELAAHPLRVGLSMDADINTHSAEA
                     P"
     gene            complement(3910..>4569)
                     /locus_tag="VFF98_08480"
     CDS             complement(3910..>4569)
                     /locus_tag="VFF98_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU63706.1"
                     /translation="GLPANASTDLIARRPDVAAALARVQADDEHIREARAGFYPALSI
                     SGLAARPALSWADFVKGGAFVGGVGPSVSLPIFHGGEIKGKYRTARGTYDEAVANYDK
                     TLANAFHEVADAAASQAALAGRLGAASQALTDAEGAYAVARQRYEGGLSTYLNVLSAE
                     EQVLSARRLHADLAARAFTLDVALVRALGGGVETTTAALSPAAPAPGAATAPSKDPHH
                     G"
BASE COUNT          707 a         1480 c         1692 g          690 t
ORIGIN      
        1 ccgggcgccg ggccatgccc gccggcaggg tgggggccag ctctgcctcc agcaggcgca
       61 cgcggtcgaa atgggcgcac aggcggcggc cgaggtcggt gggctggcag ggctgggtgc
      121 gggcgatcag gctggcgccc agtcgttcct ccagcgcgcg cacccgctgc gacaccgccg
      181 agggcgagat gcacagcgcc gccgccgccc gctcgaacgt gccttcccgg atcaccgccg
      241 agacgacggc aagggccgca taatccagca tggtatgaag catagcttca ttcacttcag
      301 caaatcaaca ttcccttgcg gccccgccct gcccataagc gcgccatgac cctgcttttc
      361 ccccccttcc tcgccggctt tgctttgtgt gcggcgctta tcgtggccat tggcgcgcag
      421 aacctgttcg tgctgcgcca ggggctgctg cgcgcccatg tggggccgat cgtgctgttc
      481 tgcatcgccg ccgatgtggc gctgatcggc gcgggggtgg ggggcgtggc ggcggccttg
      541 cgcgcgctgc cgcaactgac gctggggctg acgctgggcg gcgcggcgtt cctcgcctgg
      601 tatggcgtgc aggcgctgca gcgcatggcc gcgggcgagg cggtgaccgt ggccgccggc
      661 caggcccagc cgctggcggg cgcgctggcg gcggtggcgg gcttcacttt cctcaacccc
      721 catgtctatc tcgacacgat gctgctgatg ggggcggctg gcgctgcctg gccggcgccg
      781 ctgcggccgc tgttcgtggc tggcgcgggc agcgccagcg ccctgtggtt tgtggcgctg
      841 ggctatggcg cgcggctgct ggcgccggtg tttgcccggc ccgctgcctg gcgggtgctt
      901 gatgggctgg tgggggcaac gatgctggcg ctggccgcct cgctggcgct gcgcgcgctg
      961 tagcgggggc ctaatgcgcc gcgctggtat ccaccgcatg gcccgggcgc ggcgccagcc
     1021 agatgatcag cgccgagacg acgaagatca gcgaggagag cacgaacagg ttggtgaagg
     1081 agatcatgtc ggcctgggtg gtcaccgatt gggcgatggt gccgcggatc tgctcgaagg
     1141 gcatgccgct gctgctcagc gcatcgatgg tgggctggct gttcagcttc gagacgatct
     1201 cgctgcgtgc ggtggtggcg ccatcctcgt agagcgtggt ggagacagag gtgccgatgg
     1261 cgcccgccat ggtgcgcatg aagctcatga tgccggcggc cgaagcggtt tcttcggggc
     1321 gcaccgcccc aaggccgatg gtcgtcagcg ggatgaagaa gaagggcatg ccggcgcctt
     1381 gcagcagctg gggcatggcc atcgtccaga aatcgacatc gctgctccac agcgtgcgca
     1441 ggaaggcggt gaagcccatc cacaggatgc cgatgaacac cagcatgcgc ggatccacct
     1501 tgtccagcag cttggcgacg atgggcgaca tgatcaccgc gcacacgccc gaaaaggccg
     1561 tgacatagcc cgccttggtg gcggtgtagt tcatgctggt ctgcagccat tgcgggatga
     1621 tcaccgccga ggcgaagaag gcggcaaagg caaaggacag gctgagcacc gagacggtga
     1681 agccgcgata gcggaagatg cgcaggtcca cgatcggcgc gcgatcggtg atttcccagg
     1741 caatgaagaa cagcaccacc accccggcga taaccgcaag gcaagtgatc aggctgctgc
     1801 cgaaccagtc gtgctcgcgg cccagatcca gcatgatctg cagcgcgccg atccacacca
     1861 ccagcatcgc caggcccatc ttgtcgaccc gcaggtcggc cagttccgtc tcggccgggc
     1921 gcagcaggcg ataggtgcca aagacgcata gcgcggccac cgggatgttg atgaagaaga
     1981 tccagttcca cgaccagttg tcgctgatcg tgccgcccag gatcggcccg aggatcggcg
     2041 caacgacggt ggtcatcgcc cacaggccca tcgcctggcc atgctgctca cgcgggaaga
     2101 tgcgcagcat cagcgtctgg ctcagcggca tcagcgggcc gccgcacagc ccctgcccca
     2161 cgcggcacag cacgatcatc gccagcgagg tggacaggcc gcacagcacc gagaacacgc
     2221 caaagcccac catgccgaac atgaaggtgc gcaccgtgcc aaagcgcccg gccagccagc
     2281 cggtaagcgg cacgcagatc gcctcggcca cggaatagga cgtgatcacc caggtgccct
     2341 gcgtggagga aatgcccagc gcgccggcga tatgcgccac cgacacattg gcgatggtcg
     2401 tatcgagcac gaccatgaaa ttgctgagcg ccagcacagc gccggccagc agcagggtgg
     2461 cgccggtgat ctgtgcgggc ttggtgtggg caaccatgtt ctgtctcctg cccgaagggg
     2521 cgcaggccgg cctggtacaa aaccgtactg gcgggttcgg ttgcgcaagg gcggcgtggg
     2581 gccgggcctt catggcaaac cataccgcgc ggtgcggcga aagaaggggc ccggcaaggc
     2641 tgggaggggg ggagggcctg ccgggccaga ggcccccttg cggcaccttc gggggaaggg
     2701 ggatcttgcc gccagggggt gtgggccgaa gcgctttagg gggcttcggc ggaatgggtg
     2761 ttgatatcgg cgtccatcga caggcccacg cgcagcgggt gggcggccag ttcgcgcggg
     2821 tccagcgcca cgcgcacggg caggcgctgc accaccttga tccagttgcc ggtggcattc
     2881 tgcgccggga tcagcgcaaa ggcagagccg gtgccgcccg agaagccctg caccttgcca
     2941 tggaacacca cgccgctgcc ataaaggtcg ctggtcagct ccacgctctg gcccgggcgc
     3001 accttgtcga gctggccttc cttgtagttg gcatcgacat agagcgcgtt caccggcacc
     3061 acccgcatca gcgtggcgcc ggcggccacc cgctggccca cctgcaccgc gcgcgaggta
     3121 acgatgccat caaccggcgc gcggatgagg gtgcgttcga gatccagctt ggccttgtcc
     3181 accttggcct gggctgccag cacatcgggg ctggtggcca tggtggtgcc ggcgatcagc
     3241 gcgttggtgg cggcaaaatc gccctgggcg gcgccatggc tggcgctggc ctgggcaacg
     3301 ccggcgcggg ccagttccag attggcgcgg gctgcggcaa aggcattgac ggcgctggtc
     3361 agctcgtcgc cggaaacggc gccgctgggg gccagcttgc ggcggcgggc aaggtcgata
     3421 ttggccttgt cgtaagcggc ctgggccgag acgagctggg cctgggcgct ggcgatggcg
     3481 gcggcgcggg ccttggtctg cgcatccagc gcggcgctgg tgaagctggt ctggccatac
     3541 tggcggcggg ccttggccag gtctgcctcg gcctgggcaa gggcgatctg ggcatcgcgc
     3601 gggtcgatcc gcacgagaat gtcgcccttg tgcacggcct gggtgtcgcg cacctccact
     3661 tcggccacct ggccttccac catcggggtg atctgggcgg tgtcggcgcc gacataggcg
     3721 ttgtcggtag tgatatggtg gctgccgacc agcgcgtacc agccgccagc ggccagtgcc
     3781 gccagcgcga tgccgccggc aatgccgctc agcaggcggc ggcgggcggc ctggcggcgc
     3841 gcctcggcga tggattcgat gatctcggga tcgcccttgg gggcatccag cagcgcggtg
     3901 ggatcggctt cagccatggt gggggtcctt ggaaggggcg gtggcagcgc caggcgcagg
     3961 cgccgcggga gagagggcag cggtggtggt ctcaacgccg ccacccagcg cgcgcaccag
     4021 cgcgacatcc agcgtgaagg cgcgggcggc aagatcggca tgcaggcggc gggcgctcag
     4081 cacctgttcc tcggccgaca gcacgttgag ataggtggac aggccgcctt cgtagcgctg
     4141 gcgggccacg gcataggcgc cttcggcatc cgtcagcgcc tggctggcag cacccagccg
     4201 cccggcgagc gcggcctggc tggcggcggc atcggccacc tcgtggaagg cattggccag
     4261 ggtcttgtcg taattggcga cggcctcgtc ataggtgccg cgggcggtgc ggtatttgcc
     4321 cttgatctcg ccgccatgga agatcggcag gctgaccgag gggccaacgc cgcccacaaa
     4381 ggcgccgccc ttgacgaaat cggcccagct gagcgccggg cgcgcggcca gccccgagat
     4441 cgacagcgcc ggatagaagc cggcgcgcgc ctcgcggatg tgctcgtcgt cggcctgcac
     4501 ccgggcgagg gcggcggcca catcggggcg gcgggcaatg agatcggtgc tggcattggc
     4561 cggcaggcc
//
LOCUS       DASRYE010000162        22150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_238745,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000162 DASRYE010000000
VERSION     DASRYE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 22150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22150
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238745"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VFF98_08485"
     CDS             <1..1433
                     /locus_tag="VFF98_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU63707.1"
                     /translation="FHALDEAEFLLESAVMLAPEDTGARYDYAQVLNKRQKFAQALAQ
                     AEQLRALDGDSLQIDSLYADQNLAVGNFVAALEVYRARVAAHPRNPSYHLTLGHVLKT
                     VDAIPEAVAAYRAAYGHRPDYGVAYWSLANLKTYRFSDAELGQMRRAEADAATGLVDR
                     YHLCFALGKALEDRGEHAESFAYYDTGNRLKREETGYDWRRISAEIDQQIAHCDAGLF
                     ARHAGAGHDAPDPVFILGLPRAGSTLIEQILSSHSQVEGTMELPNILALAHRLDGKRR
                     VGEPARYPANLAELPREALAEMGRAFLADTAIHRKAGTPFFIDKMPNNFRHIGLIHLI
                     LPNARIIDARRSAMGCCFSGFKQLFAEGQEFTYGLEEVGHYYRDYVRLMDHWDKVLPG
                     KVLRVQYEDVVADLDGQVRRLLAFLGLPFEEGCIAFHRTERAVRTASSQQVRQPIYTG
                     GVDQWQHYAPWLAPLRAALGPELAQG"
     gene            1438..2958
                     /locus_tag="VFF98_08490"
     CDS             1438..2958
                     /locus_tag="VFF98_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63708.1"
                     /translation="MILPRAQAWLPWAGLVALAGALYNAAFLGTAAGIDQLITADTLL
                     PVQMAWDMHHHPWAWAGFQWPRVPSLLPDMAWFFAAEWLGLGWRRAMLGWSWLAIGLL
                     SLGLGGLVAQLRAEPASRASARVAAALALAIAAVGLLCAAGAPAVLWFPLLYVALPVT
                     HGGAFILSLFAARMALGRLEGRPGRWWPLLALCWAGAASDLLFVADFLAPLAIVCLLR
                     LPPAARRDLPQLARALGLPLACALACALGWAVQLLLYRQPLPYLATAPLWWKALLLAM
                     DLAAAPWAVLLFWLLGRMGRQALEALGGAPGQSCLLAFGALAAALSVPVLLLFYNDHT
                     YSYRYALPLLWWPLLPLLADGPRAGGRWQGRLGWAALALGVGLPLAVPLRLLAWRSPL
                     EACLSALARPAGLTAGLASYWYLRPTAASADWALQIDQIDDTGARGLWGNDTAAYGHD
                     QNAPNHAPPYRFLIREPGLPAARMRTAFGPPQAIRPCPGADIWLYARPLIPAIFPR"
     gene            3096..4319
                     /locus_tag="VFF98_08495"
     CDS             3096..4319
                     /locus_tag="VFF98_08495"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZU63709.1"
                     /translation="MAKIKVANPVVEIDGDEMTKIIWQWIRERLILPYLDIDLKYFDL
                     SVENRDATDDQVTVDSANAIKEYGVGVKCATITPDEARVEEFSLKQMWKSPNGTIRNI
                     LGGVVFREPIVISNVPRLVPGWTDPIVVGRHAFGDQYRATDFKVPGAGKLRLVWEGDN
                     GEVIDREVFKFPDAGVAMAMYNLDESIRDFARASFNYGLALGWPVYLSTKNTILKAYD
                     GRFKDLFAEVFEAEGFKEKFAAAKIYYEHRLIDDMVASALKWSGKFVWACKNYDGDVQ
                     SDIVAQGFGSLGLMTSVLMSPDGKTVEAEAAHGTVTRHYRQHQQGKATSTNPIASIFA
                     WTRGLIYRGRFDNTPDVVKFAETLEKVCVATVESGKMTKDLALLIGPEQGWLTTEAFF
                     EEIVKNLETEMAAWA"
     gene            4455..6233
                     /locus_tag="VFF98_08500"
     CDS             4455..6233
                     /locus_tag="VFF98_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZU63710.1"
                     /translation="MSDPSAFSPSSPLGDALVILGAAGVVIPVFARFRITPVIGFILV
                     GLLVGPYGLGRHVFDYPWLSFVTISDADALEPFAEFGIILLLFSVGLELSFGRLWSMR
                     AQVFGLGAMELLGAGLLTSFALAFMGEAPSGALALGLALAMSSTALVLPIAGTHTAVG
                     RSALAMLLFEDLALVPILFLLSALAPHNGGGGLDGMLRTLGMGVGVVIALLVAGRILM
                     RPLFAQAARTKSPELFLAASLIVVIVASLATAGVGLSPIVGALTAGLLIAETEYHTEV
                     EGITAPFKGLALGVFLITVGMSIDLASIARHLPALLGATAALIGAKALVTSLLLRLTG
                     TRAGQAAETGLLMASPSESTLIVLGAATTGGLIQPGTAQFWQIVTALGLTITPLLAMA
                     GRAIARRVDRHAHAAPETDDDASNAAIIVGFGRVGQLVADMLARHDKPYIAIDSNPDL
                     IAEGLRCGYRVAFGNAASPHSWRKLGVGAASAVILTMDEPITAARLVRRLRAQYPDLP
                     ILARARDPGHAVQLYKAGATQAIPETLESSLQLSESVLVELGVPMGFVIASIHEMRDE
                     FREQIMQEGGLQEKPRLKSSSLRERS"
     gene            complement(6281..8947)
                     /gene="alaS"
                     /locus_tag="VFF98_08505"
     CDS             complement(6281..8947)
                     /gene="alaS"
                     /locus_tag="VFF98_08505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZU63711.1"
                     /translation="MTSTNEIRRSFLDYFGAHGHDVVPSAPLVPYNDPTLMFTNAGMV
                     PFKNVFTGLERRAIPRATSSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEQAITHAWTLLTREWGLSKEKLLVTVYHTDDEAAQLWRKIAGLGDERIIRIPTSDNF
                     WSMGDTGPCGPCSEIFYDHGEAIPGGPPGSPDEDGDRFIEIWNLVFMQFETLADGNRV
                     DLPRPSIDTGMGLERIAAVLQGEHDNYDIDTFRQLIAASESLTGVKAEGEHRASHRVI
                     ADHLRSTSFLLADGVLPSNEGRGYVLRRIMRRAMRHAHLLGAKEPLMHRLVPALVTEM
                     GQAFPELGRAQALIQEVLEREETRFRQTLEKGLKLLDEATSGLGAGAKLAGETAFRLY
                     DTYGFPYDLTEDALRARDISVDREGFESAMAQQKAAARAAWKGSGEAAAGEVWFDIAE
                     RVGATEFTGYTATQGEGVVVALVKDGAEVDSAAAGDTVVVITNQTPFYGESGGQLGDA
                     GVISGIANGDNRDLKVVIGDTAKPLGRLHAHSGVVEAGVIRVGDAVQLAIDVARRNAI
                     RANHSATHLLHQALRNRLGEHVTQKGSMVAPERLRFDFSHPTGLSAEDIAAVEAEVNA
                     EIRANDAVVTRLMSPEEAITEGAMALFGEKYGEEVRVLSMGSRHDGRTYSTELCGGTH
                     VRALGDIGVFRVVGESAVSSGVRRIEALTGEGARQWLVGREEQLKAVAASLRASPEEV
                     EARVAALVDERKKLERELAEAKKALALSGGGAAQAAGPEQIGDVSYFAQIIDGLEPKE
                     LKPLADDKRRELGSAVITLIAVNEGANTVVQAATADLAERFDASKLIRIATEAMGGKG
                     GGGRPDMAQGGGPAGREAAEAAIAAVKAVIAG"
     gene            complement(9109..10179)
                     /gene="recA"
                     /locus_tag="VFF98_08510"
     CDS             complement(9109..10179)
                     /gene="recA"
                     /locus_tag="VFF98_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZU63712.1"
                     /translation="MAAQLKLIQEGKDKDMDRQKALDAALAQIDRAFGKGSAMKLGSR
                     EAMQIEAISTGSLGLDIALGIGGLPRGRIVEIYGPESSGKTTLALHAIAEAQKKGGTA
                     AFVDAEHALDPVYAKKLGVDIDELIVSQPDTGEQALEIVDTLVRSNAIDVLVVDSVAA
                     LVPRAEIEGEMGDSHVGLQARLMSQALRKLTGSISRSRCMVIFINQVRMKIGVMYGNP
                     ETTTGGNALKFYASVRLDIRRTGQIKDRDDIVGNATRVKVVKNKVAPPFKQVEFDIMY
                     GEGISKVGEILDLGVKAGIVEKSGAWFSYDSIRIGQGRENAKNYLRENTEVRERLEAA
                     IRAQTEGLAGEMMAGPDGEEES"
     gene            complement(10336..12708)
                     /locus_tag="VFF98_08515"
     CDS             complement(10336..12708)
                     /locus_tag="VFF98_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU63713.1"
                     /translation="MWPAAAALIVVTALLWLVFHEGVVVLSFLGGVAVLGALALKLGA
                     DAQARPVPALAQPDWSVTVAAMERPDTAVAVVDRAGRLVCASSLWESWFSLAHAPLAP
                     GLDGPSQAAMQEAARQALRDGAAGAVALRDGSGRQWQASVKRCGRAEDFLIWQAGAVV
                     RVDPLAQVAQAVTGAAGALFAEAGFAAALVSPEGRVHAANEAFLHHAGTQADFALLLT
                     QDEHARVYWASDSHRTRPLIVYCLPLAGGEQAPALMLLVEHGQGVGAAGMTAGAAPHL
                     EALLAPLPLGLAMADRDGRLLFANAAFMRAAGHEGEAPPVWPIDLVVREDKAALADAV
                     RRFAQGAVISGDVAVRLAAQPEDPVTMSLAGVRGLGEAAVLLSLIDTTEEARLKRQVA
                     QATKMQAVGQLAGGVAHDFNNVLTAIIGTCDLMLLRHTPGDSDYDDIQQIRASSNRAA
                     SLTRQLLAFSRQQTLRPEVLQLPDIVSEISQMLRRLIGEKIVLEVHHDRQLGPVRADP
                     TQLEQVIVNLVVNARDAMQDYSAKVGGDGAGRLVLATRRVAAGDVRALRSDILPIADY
                     TALIVEDTGGGIPADILGKIWEPFFTTKEQGRGTGLGLSTVYGIVKQSGGFIFAENVA
                     GADGRVSGARFTVYLPVHHGAEAAPQRAAESAAAWAGGGDILLVEDEDAVRMVAERAL
                     LRAGYQVTCARDGEDGLAEVRERLGGGGPMFDLVLSDVVMPAMDGPAMARAIRKLAPG
                     VPLLFMSGYAEEQLRREIDIENMHFMPKPFSVGQIAGKVAEVLAEAARGR"
     gene            complement(12810..13388)
                     /locus_tag="VFF98_08520"
     CDS             complement(12810..13388)
                     /locus_tag="VFF98_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZU63714.1"
                     /translation="MTRGGQQAGALKRLLAHAVPTREKLESNRWLRPFAHLVLRPGLW
                     RFNRRSVPRGVALGLFVGILIPFAHSFVAALVAVAVRANIPVAVATTLLSNPATWVLI
                     FPVAYKVGKFLLHADAAMSGQPVSSLMSAGDHTEMLHRLTGKGLALAYGLLVEATVVA
                     ALGYVLTSVIWRCIVTRRRRRRLGSGIAVGRA"
     gene            complement(13385..13867)
                     /gene="smpB"
                     /locus_tag="VFF98_08525"
     CDS             complement(13385..13867)
                     /gene="smpB"
                     /locus_tag="VFF98_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZU63715.1"
                     /translation="MARPTPATFDKKKTVAENRRARFDYFIDDTFEAGIALTGTEVKS
                     LRFGEGSIAEAYAEVKNGEVWLVNANVPEFSHGNRFNHEPKRPRKLLLHAREIDKLTG
                     AVERKGMTLVPLSVYFNGRGRAKVELALAKGKNVADKRATIKERDWKRDQQRILRDYK
                     "
     gene            complement(14063..15280)
                     /locus_tag="VFF98_08530"
     CDS             complement(14063..15280)
                     /locus_tag="VFF98_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZU63716.1"
                     /translation="MSIHLDAPAVVRDFAPNEPVILNRPHAAERAARFFAETFPGKSL
                     YAVKANPSPELLRVLWAGGITHYDTASITEVRLVRETLGDAPVLCFMHPVKPFAAIAA
                     AYHTYGVRTFSLDTHEELEKIVEATRDAEGNAATDLSLCVRLRVSSEYSELSLASKFG
                     VDLVDAAPLLQATRQVADCLGICFHVGSQAMTPFAYVQALERARAAIVDASVTVDIID
                     VGGGFPSIYPGMTPPPLEDYFGAIHRAFESLPVSYSSELWCEPGRALSAEFSSMIVKV
                     EKRRGNELFINEGAYGALYDAAHVDWRFPVRAIGADGVEKDGPAGEFAFYGPTCDDAD
                     YMEGPFALPAGIEAGDYIEVGMLGAYGVAMRTKFNGFGAGLTIEAADEPMASQYLGDR
                     RDLRRADNVVSLR"
     gene            complement(15531..16715)
                     /locus_tag="VFF98_08535"
     CDS             complement(15531..16715)
                     /locus_tag="VFF98_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HZU63717.1"
                     /translation="METRAGDPGAFAHFDLGRVDSPAFVVDAARLRANLAVLADIKAR
                     AGVKVLSAMKAFSMWKVAPLIGDYLDGVCTSGLWEARLARDYYRGEIATYCAGYKAAD
                     MAEICAISDHVIFNSPMQAARFAPELARARAEGASFDVGLRLNPLHREGEVEKYDPCA
                     LGSRLGFPVSQLRAEHLEGVSGLHFHTLCEQDFEPLKRTWEALAPRIAPWLAANGGPI
                     RWLNFGGGHHITRADYQRDELVDFLIRVRAETGCTLYLEPGEAVALDAGILVGSILDA
                     HENGMPVAIVDISATCHMPDVIEAPYRPAMLGELSEGPEVRLGGPSCLAGDIIGDYRL
                     PVPAEPGQRFAFLDQAHYSMVKTTTFNGVPLPSIWLWDSADDSLECVKKFDWTTFRDR
                     LG"
     gene            complement(16722..17066)
                     /locus_tag="VFF98_08540"
     CDS             complement(16722..17066)
                     /locus_tag="VFF98_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HZU63718.1"
                     /translation="MNWGLILPPLMLAGSNLFMNVAWYGHLRAPHKAIWVAVLASWGL
                     AFCEYCLAVPANRIGARAYSLPQLKTMQEVFSLAGFVVVAWALFGQKPGWPQLAGFAL
                     IIAGAGLIFKGN"
     gene            complement(17083..18294)
                     /locus_tag="VFF98_08545"
     CDS             complement(17083..18294)
                     /locus_tag="VFF98_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HZU63719.1"
                     /translation="MSKVLVIGAGGVSSVAVHKMAMNAAIFPEIHLASRTKAKCDAIA
                     ESVKQRTGETVATYAIDAEDVPALSALIRQIGPKLVVNLALPYQDLTIMDACLAAGAH
                     YLDTANYEPKDVARFEYHWQWAYQERFKAAGLMALLGSGFDPGVTSVFAMWLKKHKLK
                     TIRQLDILDCNGGDHGQAFATNFNPEINIREVTAPARHWEGGAFVETPPMAKKVSFDF
                     EGVGPKNMYMMYHEELESLAKFLPELERGRFWMTFGDEYIKHLTVLQNVGMTRIDPVL
                     YNGVEIVPLQFLKAVLPEPSTLGATTKGKTNIGDIATGEALDGSGEKTFYIKNICDHE
                     ACFEETGNQAVSYTTGVPAMIGAALIMQGVWDGEGVFNMEQMDPDPFMALLNTQGLPW
                     TVEELEGPVAF"
     gene            18610..19878
                     /locus_tag="VFF98_08550"
     CDS             18610..19878
                     /locus_tag="VFF98_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HZU63720.1"
                     /translation="MSAIPASPDLSSGQLRAGHPKAARVIEQAVVRRHVQAKERLLDK
                     AFALAFRGLVYAQIWEDPVIDMEALDIQPDNRIVTIASGGCNALSYLTANPAKITAVD
                     LNTAHVALNKLKFAAVRQLPDYTALRRFFVEADNKANVGAYRKYLRPHLDEATRRYWE
                     GRDLFGRRRILGFAKGIYKRGLLGSFIGMAHLVAKAYRVDPRVILEARTLEEQRRVFD
                     EKFAPVFDKRFIRWLTSQPASLFGLGIPPAQYDALAGDQRMAHVLRARLEKLACDFSI
                     HDNYFAWQAFGRGYGKAESAPLPPYLQAQNYDALRERIDRIEVLHCNFTEYLASQRDA
                     SLDRYILLDAQDWMSDLQLTFLWREITRTARPGARVLFRTAAEETLLPGRVPAEILDR
                     WDYSEAEAQRLIRADRSSIYGGLHLYRFKG"
     gene            19884..20537
                     /locus_tag="VFF98_08555"
     CDS             19884..20537
                     /locus_tag="VFF98_08555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU63721.1"
                     /translation="MASIAPATGGHGQLMDNVYRWQRHIYDATRKYFLFGRDRLIAGL
                     DLAEGGTLLELGCGTGRNLDLAARHWPAASLHGLDISAEMLKSAEAKLGARAQLAEGD
                     ACAVEADALFGRARFDRVMISFALSMIPAWEAALAEGLRLLAPGGSLHIVDFGDAAGL
                     PAPLRALLNGWLARFHVSPRLTLADRATALAATGRFKVRTRRGPFGYYQLIVLTRRS"
     gene            complement(20534..21730)
                     /locus_tag="VFF98_08560"
     CDS             complement(20534..21730)
                     /locus_tag="VFF98_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZU63722.1"
                     /translation="MEEMIIRIAARGDGATASGRHVAGAAPGDVVLADGALRKGPHHA
                     EPACRHYGQCGGCQLQHVDDAALATFVGERVAGAAAGQRLVPDVLAPVHLSPRGSRRR
                     AALHAARVGGAVALGFREGQSHRLVDIGECPVLAPELVALIAPLRRMLARQDGRLSVE
                     IDMTLADQGVDLALKGLTVEGLARTEAWLAFAREQQLARLSLDQGYGPESLWEPDGVT
                     ITLGEIAVPLPPGAFLQATADGEAALVGAAVAWLEGSGVVADLFSGLGTFALALAKAG
                     HKVLAAEADRAAHLACKGAAARALLPVAALHRDLFRNPLLPEELNRFGAVVLDPPRAG
                     AREQVGRIVASTVARVVYVSCNPASWARDAAQLQAAGYRLVELRPVGQFRWSTHVELA
                     SLFVRG"
     gene            complement(21745..>22150)
                     /locus_tag="VFF98_08565"
     CDS             complement(21745..>22150)
                     /locus_tag="VFF98_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZU63723.1"
                     /translation="DALPEGAIATPHEGELAAMERAFGLSGAGAKWQRAQALAQASGL
                     VILAKGPDSLIAAPDGRLLCAARAPSWLSVAGSGDVLAGCVAARLAAGEDAFAAAATA
                     LWLHGEAARLAGGPFTAGQLAGMVRAAYRTAL"
BASE COUNT         3653 a         8012 c         6872 g         3613 t
ORIGIN      
        1 gcttccatgc gctggacgag gccgagttcc tgctggaatc cgccgtgatg ctggcgccag
       61 aggatactgg cgcgcgctat gattatgcgc aggtgctcaa caagcggcag aaattcgccc
      121 aggcgctggc ccaggccgaa cagctgcgcg cgctcgatgg cgacagcctg cagatcgatt
      181 ccctctatgc cgaccagaac ctggccgtcg gcaatttcgt ggccgcgctg gaagtgtacc
      241 gcgcgcgcgt ggccgcccat ccgcgcaatc cctcctatca cctcaccctc ggccatgtgc
      301 tgaagacggt ggacgccatc cccgaggccg tggccgccta tcgcgccgcc tatggccaca
      361 ggcccgatta tggcgtggcc tactggagcc tcgccaacct caagacctat cgcttttccg
      421 atgccgaact gggccagatg cggcgggccg aggccgatgc cgccactggc cttgtcgatc
      481 gctatcacct gtgtttcgcg ctgggcaagg cgctggaaga ccgcggcgaa catgccgaaa
      541 gctttgccta ttacgacacc ggcaaccgcc tgaagcgcga ggagaccggc tatgactggc
      601 gccgcatctc ggccgagatc gaccagcaga tcgcccattg cgatgccggc ctgtttgccc
      661 gccacgccgg cgccggccat gatgcgcccg atccggtctt cattctgggc ctgccgcgcg
      721 ccggctccac gctgatcgag cagatcctct ccagccattc gcaggtggaa ggcacgatgg
      781 agctgcccaa tatcctggcg ctggcccacc ggctggatgg caagcgccgg gtgggcgagc
      841 cggcccgcta tccggccaat cttgccgagc ttccgcgtga ggcgctggcc gagatgggcc
      901 gcgccttcct ggccgacacg gccatccacc gcaaggccgg cacgcccttc ttcatcgaca
      961 aaatgcccaa caacttccgc cacatcggcc tgatccacct gatcctgccc aatgccagga
     1021 tcatcgatgc gcgccgctcg gcgatgggct gctgcttctc cggcttcaag caattgttcg
     1081 ccgaggggca ggagttcacc tatgggctgg aggaagtggg ccattattac cgcgattatg
     1141 tgcggctgat ggaccattgg gacaaggtgc tgcccggcaa ggtgctgcgc gtgcaatatg
     1201 aggatgtggt ggccgatctg gacgggcagg tgcgccggct gctggccttc ctcggcctgc
     1261 ctttcgagga gggctgcatc gccttccacc gcaccgaacg cgccgtgcgc accgccagct
     1321 cacagcaggt gcgccagccc atctacaccg gcggggtgga tcagtggcag cattacgccc
     1381 cctggctggc ccccttgcgc gccgcgctgg ggccagaact ggcgcaaggc tgaaggcatg
     1441 atcctgccca gggcacaggc atggctgccc tgggcagggc tggtggcgct ggccggcgcg
     1501 ctctacaatg ccgccttcct tggcaccgcc gccggcatcg accagctgat caccgccgac
     1561 acgctgctgc ccgtgcagat ggcctgggac atgcaccacc acccatgggc atgggccggc
     1621 ttccagtggc cgcgcgtgcc cagcctgctg cccgacatgg cctggttctt cgcggccgaa
     1681 tggctggggc tgggctggcg gcgagccatg ctggggtgga gctggctggc gatcggcctg
     1741 ctttcgctgg ggctgggcgg gctggtggcg caactgcgcg cagagcctgc ctcccgcgcc
     1801 agtgccagag tggccgcggc cctcgctctg gcgattgccg ctgtggggct gctgtgtgcg
     1861 gcgggggccc cggcggtgct gtggtttccg ctgctttatg tggcgctgcc ggtcacgcat
     1921 gggggggcgt tcatcctgtc gctgtttgcc gcgcggatgg cgctggggcg gctggaaggg
     1981 cgccccggcc ggtggtggcc gctgctggcg ctgtgctggg cgggggcggc gtccgatctg
     2041 ctgttcgtgg ccgatttcct ggcgccgctg gctatcgtgt gcctgctacg cctgccgccc
     2101 gcagcccggc gcgatctgcc gcagctggcc cgcgcgctgg gcctgccgct ggcctgcgcc
     2161 ttggcctgcg cgctgggctg ggcggtgcaa ttgctgctct atcgccagcc actgccctat
     2221 ctggccaccg cccccttgtg gtggaaagcg ctgctgctgg ccatggatct ggcggccgcg
     2281 ccctgggcgg tgctgctgtt ctggctgctg gggcgcatgg ggcgccaggc actggaggcg
     2341 ctggggggcg cgccgggcca gtcctgcctt ttggcttttg gcgcgctggc agcggcgctg
     2401 tcggtgccgg tgctgctgct cttctacaat gatcacacct acagctatcg ctatgcgctg
     2461 ccgctgctgt ggtggccgct gctgcccctg ctggcagatg ggccgcgggc cggcgggcgc
     2521 tggcaggggc ggctgggctg ggcggccttg gcgctgggcg tgggcctgcc gctggccgtc
     2581 cccctgcgcc tgctggcctg gcgcagcccg ctggaggcgt gcctgtcggc gctggcgcgc
     2641 cccgccggcc ttaccgccgg ccttgcttcc tactggtatc tgcggcccac cgccgcctcg
     2701 gccgactggg ccctgcagat cgaccagatc gacgataccg gcgcgcgcgg cctctggggc
     2761 aacgacaccg ccgcctatgg gcatgaccag aacgccccga accatgcgcc cccctatcgt
     2821 ttcctgatcc gcgagcccgg cctgccggcg gcccgcatgc gcacagcctt tggcccgcca
     2881 caggccatcc gcccctgccc cggcgccgat atctggctct atgcgcggcc gctgatacct
     2941 gccatttttc cgcgctgatt ggcccaaaac ccgcaaaaac agcaggtttg tcgccagttg
     3001 caaatcaata gcaagccatt gcgccctgcg accttgcgcc gcagcaatgc tcttactaag
     3061 aggcctcgtt acctctatcc ccaggaaggc aaattatggc caagatcaag gttgccaacc
     3121 ctgtcgtcga gatcgacggc gatgaaatga cgaagatcat ctggcagtgg atccgcgaac
     3181 gcctgatcct gccctatctc gacatcgacc tgaagtattt cgacctctcg gtggaaaacc
     3241 gcgacgccac cgacgaccag gtgaccgttg attcggccaa tgccatcaag gaatatggcg
     3301 tgggcgtgaa gtgcgccacc atcacgcccg acgaagcccg cgtcgaggaa ttcagcctca
     3361 agcagatgtg gaagagcccc aacggcacca tccgcaacat ccttggcggc gtggtcttcc
     3421 gcgagcccat cgtgatctcg aacgtgccgc gcctggtgcc cggctggacc gacccgatcg
     3481 tcgttggccg ccacgctttt ggcgaccagt accgcgcgac cgacttcaag gtgccgggcg
     3541 ccggcaagct gcgcctcgtc tgggaaggcg acaatggcga agtgatcgac cgcgaggtct
     3601 tcaagttccc cgacgcgggc gtggccatgg ccatgtataa cctcgacgaa tcgatccgcg
     3661 actttgcccg cgcctcgttc aactatggcc tggcgctggg ctggccggtg tatctctcca
     3721 ccaagaacac catcctcaag gcctatgacg gccgcttcaa ggatctgttc gccgaggtct
     3781 tcgaggccga aggcttcaag gagaagttcg ccgccgccaa gatctactac gagcaccgcc
     3841 tgatcgacga catggtcgcc tcggcgctca agtggtcggg caagttcgtc tgggcctgca
     3901 agaactacga cggcgacgtg cagtcggaca tcgtcgcgca gggcttcggc tcgctgggcc
     3961 tgatgacctc ggtgctaatg agccccgacg gcaagaccgt ggaggccgag gccgcccatg
     4021 gcaccgtgac ccgccactat cgccagcacc agcagggcaa ggcgaccagc accaacccga
     4081 tcgccagcat cttcgcctgg acgcgcggcc tcatctatcg cggccgtttc gacaacacgc
     4141 ccgatgtggt gaagtttgcc gagacgctgg agaaggtgtg cgtggccacc gtcgaaagcg
     4201 gcaagatgac caaggatctg gcgctgctga tcggccccga gcagggctgg ctgacgaccg
     4261 aggccttctt cgaggaaatc gtcaagaacc tcgaaaccga aatggcggcc tgggcgtaac
     4321 acacgccctc cggcttgcgg atgaaagggg cccggggttt tcctcgggcc ccttttgcat
     4381 ggccgcctgc ccgaaggcgc cgccggccgc ccccaccctc ttgcatcccg cgccccgatg
     4441 cctaggctgg ggccatgtct gatccttccg ctttctcgcc atcctcgccg ctgggcgatg
     4501 cgctggtgat cctgggcgcg gccggggtgg tgatccccgt tttcgcgcgc tttcgcatca
     4561 cgccggtcat cggcttcatt ctggtggggc tgctggtggg cccctatggc ctgggccgcc
     4621 atgtgttcga ttatccctgg ctcagcttcg tcaccatttc ggatgccgat gcgctggaac
     4681 cctttgccga attcggcatc atcctgctgc tgttctcggt ggggctggag ctgagctttg
     4741 gccgcttgtg gtccatgcgc gcgcaggtgt ttggcctggg cgcgatggag ctgctgggcg
     4801 caggcctgct caccagcttt gccctggcct tcatgggcga ggcgcccagc ggcgcgctgg
     4861 cgctgggcct ggccctggcg atgagctcca ccgcgctggt gctgccgatc gccggcaccc
     4921 acacggcggt gggccgctct gctctggcca tgctgctgtt cgaggatctg gcgctggtgc
     4981 ccatcctctt cctcctttct gccctggcgc cgcacaatgg cggcggcggg ctggatggca
     5041 tgctgcgcac gctgggcatg ggcgtgggcg tggtgatcgc gctgctggtg gccggccgca
     5101 tattgatgcg cccgctgttt gcccaggccg cccgcaccaa aagcccggaa ctgttcctgg
     5161 cggccagcct catcgtcgtc atcgtcgcct cgctggccac ggcaggcgtt ggcctgtcgc
     5221 ccatcgtcgg cgcgctgacc gccggcctgc tgatcgccga aaccgaatat cacaccgagg
     5281 tggaaggcat caccgcgccc ttcaaggggc tggcgctggg cgtcttcctg atcaccgtgg
     5341 gcatgagcat cgaccttgcc tcgatcgccc gccacctgcc cgcgctgctg ggcgccaccg
     5401 ccgcgctgat cggcgccaag gcgctggtca ccagcctgct gctgcgcctc accggcacgc
     5461 gcgcgggcca ggcggcggaa accggcctgc tgatggccag cccctcggaa tcaacgctga
     5521 tcgtgctggg cgctgccacc accggcggcc tgatccagcc cggcaccgcg cagttctggc
     5581 agatcgtcac ggcgctgggc ctcaccatca cgccgttgct ggccatggcc ggccgcgcca
     5641 tcgcccgccg ggtggaccgc cacgcccatg ccgcccccga aaccgacgac gatgccagca
     5701 atgccgccat catcgtcggc tttggccgcg tgggccagct ggtggccgat atgctggccc
     5761 gccacgacaa gccctatatc gccatcgatt ccaaccccga tctcatcgcc gagggcttgc
     5821 gctgtggcta tcgcgtggcc tttggcaatg ccgcctcgcc gcacagctgg cgcaagctgg
     5881 gggtgggcgc ggccagcgcc gtcatcctca ccatggacga gccgatcacc gcggcccggc
     5941 tggtgcgccg cctgcgcgcg caatatcccg atctgccgat cctggcccgc gcgcgcgatc
     6001 cgggccatgc cgtgcagctc tacaaggccg gcgccaccca ggcgatcccg gaaacgctgg
     6061 aatcctcgct gcaactgtcg gaaagcgtgc tggtggaact gggcgtgccg atgggcttcg
     6121 tcatcgcttc catccacgaa atgcgcgacg aattccgcga acagatcatg caggaaggcg
     6181 ggctgcagga aaaaccccgc ctcaaaagct cttccctgcg cgaacgcagc taagaaaagg
     6241 cgcggccaga aaaaaggggg cgttgcgcgc ccccttcccc ttacccggcg atcacggcct
     6301 tcaccgcggc gatcgcggcc tctgccgctt cgcgcccggc cgggccgccg ccctgggcca
     6361 tgtcgggccg gccgccgccg cccttgccgc ccatcgcctc ggtggcaata cggatcagct
     6421 tgctggcatc gaaacgctcg gccagatcgg cggttgccgc ctgcaccacc gtattcgccc
     6481 cttcgttcac cgcgatcagc gtgatcaccg cagaccccag ctcgcgccgc ttgtcatcgg
     6541 ccagcggctt cagctccttg ggctccagcc cgtcgatgat ctgggcgaaa tagctgacat
     6601 cgccaatctg ctcggggccc gccgcctgcg ccgccccgcc gccggaaagc gccagcgcct
     6661 tcttggcttc ggccagctcg cgctccagct tcttgcgctc gtcgaccagc gcagccaccc
     6721 gcgcttccac ttcctcgggg ctggcgcgca gcgatgccgc caccgccttc aactgctcct
     6781 cgcggcccac cagccactgg cgcgcgccct cgcccgtcag cgcctcgatc cgccgcacgc
     6841 cagacgacac cgcgctctcg cccaccacgc ggaacacgcc gatatccccc agcgcgcgca
     6901 catgggtgcc accgcacagc tcggtggaat aggtgcggcc atcatgcctg ctgcccatcg
     6961 acagcacgcg cacttcctcg ccatatttct cgccgaacag cgccatcgcc ccttcggtga
     7021 tcgcctcctc cgggctcatc agccgggtga cgaccgcatc attggcgcgg atctcggcat
     7081 tcacctcggc ctcaaccgcg gcgatatcct ctgccgacag gcccgtgggg tgcgaaaaat
     7141 cgaagcgcag ccgctcaggc gccaccatcg agcccttctg cgtcacatgc tcgcccagcc
     7201 ggttacgcag ggcctgatgc agcagatggg tggccgaatg gttggcgcga atggcattgc
     7261 gccgcgccac atcgatcgcc agctgcaccg catcgcccac gcggatcacc ccggcctcca
     7321 ccacgccgct atgggcatgc aggcggccca gcggcttggc cgtatcgcca atcaccacct
     7381 tgagatcgcg gttatcgcca ttggcgatgc cgctgatcac gccggcatcg cccagctggc
     7441 cgccgctttc gccataaaac ggcgtctggt tggtgatgac gacaaccgta tcgccggccg
     7501 ccgcgctgtc cacctcggcc ccgtccttca ccagggccac aacaacgccc tcgccctgcg
     7561 tggcggtgta accggtgaat tccgtcgcgc ccacgcgctc ggcaatgtcg aaccacactt
     7621 cgccggcggc cgcctcgccg ctgcccttcc aggccgcgcg ggcggcagcc ttctgctgcg
     7681 ccatcgccga ctcgaagcct tcgcgatcca cgctgatgtc gcgagcgcgc agggcatcct
     7741 cggtcagatc ataggggaag ccataggtgt catacaggcg gaaggccgtc tcgccggcca
     7801 gcttggcgcc cgcgcccagc ccgctggtcg cctcgtccag cagcttcagc cccttctcca
     7861 gcgtctggcg gaaacgggtc tcctcgcgct ccagcacttc ctggatcagc gcctgcgcgc
     7921 ggcccagctc cggaaaggcc tggcccatct cggtcaccag cgccggcacc agccgatgca
     7981 tcaacggctc cttcgcgccc agcagatggg catggcgcat ggcgcggcgc ataatgcgcc
     8041 gcagcacata gccacggccc tcattcgagg gcagcacacc atcggccagc agaaaactgg
     8101 tggaacgcaa atgatcggca atcacccgat ggctggcgcg atgctcgccc tcggccttca
     8161 cgccggtcag gctctcgctc gcggcgatca actggcggaa cgtgtcgatg tcgtaattgt
     8221 catgctcgcc ctgcagcaca gcggcaatcc gctccagccc catgccggta tcgatgctgg
     8281 ggcgcggcag atccacccgg ttgccatcgg ccagcgtctc gaactgcatg aacacaaggt
     8341 tccagatctc gatgaagcga tcgccatcct catccgggct gccgggagga cccccgggaa
     8401 tcgcctcacc gtgatcgtag aaaatctcgc tgcacgggcc gcaggggcct gtatcgccca
     8461 tcgaccagaa attgtcggag gtcgggatac ggatgatccg ctcatcgccc aatccggcaa
     8521 tcttgcgcca caattgcgcc gcctcgtcat ccgtatggta aacggtgacc agcagcttct
     8581 ccttcgacag cccccattcg cgggtcagca aggtccaggc atgggtgatc gcctgttcct
     8641 tgaaatagtc accgaaagag aaattgccca gcatttcgaa gaatgtgtgg tgccgcgccg
     8701 tatagcccac attatcgagg tcgttatgct tgccgccggc ccgcacgcac ttctgcgagg
     8761 aggtcgcccg cgggatcgcg cgccgttcaa gcccggtaaa cacgttcttg aacggcacca
     8821 tgcccgcgtt ggtgaacatc aacgtcggat cgttatacgg caccagcggc gccgaaggaa
     8881 cgacatcatg gccatgcgcc ccgaaatagt cgaggaaaga gcggcggatt tcattcgtcg
     8941 aagtcatggc acaggccgat aagctacaca gctctcgcaa acaagcggca aagccccgat
     9001 ctctctcatc accctatccc gctttctcca aagacggcgc cagccatccc ccgatgcccg
     9061 gcagcaggcg cgcgtttgcg gcccgcctgc caaacagcgc ccggcctctc agctttcctc
     9121 ctcgccatcg ggcccggcca tcatttcgcc ggccagccct tccgtctggg cgcgaatcgc
     9181 tgcctccagc cgctcgcgca cctcggtgtt ctcgcgcaga tagttcttgg cattctcgcg
     9241 gccctgcccg attcggatcg aatcatagct gaaccaggcc cccgatttct cgacgatacc
     9301 cgccttcaca cccagatcga gaatctcgcc cactttcgag atgccctcgc catacatgat
     9361 gtcgaattcc acctgcttga aaggcggtgc caccttgttc ttcaccacct ttacgcgggt
     9421 ggcattgccg acgatatcgt cgcgatcctt gatctgaccc gtgcggcgaa tgtccagacg
     9481 caccgaagcg tagaacttca aggcattgcc gcccgtcgtc gtctccgggt tgccatacat
     9541 cacgccgatc ttcatgcgca cctggttgat gaagatcacc atgcagcgcg aacggctgat
     9601 cgagccggtc agcttgcgca gcgcctggct catcaggcgg gcctgcaggc ccacgtggct
     9661 gtcgcccatc tcgccctcga tttcggcccg cggcaccagc gccgccaccg aatcgaccac
     9721 cagcacatcg atcgcgttcg agcgcaccag cgtgtcgacg atctccagcg cctgctcacc
     9781 cgtatcgggc tgcgagacga tcagctcgtc gatgtccacg cccagcttct tggcatagac
     9841 gggatcgagc gcatgctcgg catcaacgaa agccgccgtg ccgccctttt tctgggcctc
     9901 ggcaatggcg tgcagcgcca gcgtcgtctt gcccgagctt tccggcccgt agatctcgac
     9961 gatgcgcccg cgcggcaggc cgccaatgcc cagcgcgata tccagcccca gcgaaccggt
    10021 cgagatcgcc tcgatctgca tcgcctcgcg cgagcccagc ttcatcgccg agcccttgcc
    10081 aaacgcccgg tcgatctgcg ccagcgccgc atccagcgcc ttctgacggt ccatatcctt
    10141 gtccttgcct tcctgaatca gcttgagctg agccgccatg atacgtctcc ttgcctaaag
    10201 catcgcgcag gaagttgaga acaggttttt caccaatgcg atgcgcaacc atgcgcgctg
    10261 gagcggttct ccaacacgaa tcagggtgta tcgcatttgt tccgattgaa caagagaaga
    10321 acaaaaagcg ctcaatcagc gccctcgcgc cgcctctgcc aacacctcgg ccaccttccc
    10381 cgcgatctgc cccacagaga agggcttggg catgaagtgc atgttttcaa tatcgatttc
    10441 gcggcgcagc tgctcctcgg catagcccga catgaacagc agcggcaccc ccggcgccag
    10501 cttgcgaatg gcccgcgcca tcgccggccc gtccatcgcc ggcatcacca catcgctcag
    10561 caccagatcg aacatcggcc cgccgccgcc cagccgctcg cgcacttcgg ccaggccatc
    10621 ctcgccatcg cgtgcgcagg tcacctgata gcccgcgcgc agcagcgccc gctcggccac
    10681 catgcgcacc gcatcctcat cctccaccag caggatatcg ccgccgcccg cccaggccgc
    10741 ggcgctttcg gcggcccgct ggggcgcggc ctcggcgcca tgatgcaccg gcagatagac
    10801 cgtgaaacgc gcgcccgaca cccgcccgtc ggcgccggcc acattctcgg caaagatgaa
    10861 cccgcccgat tgcttgacga tgccatagac ggtggagagg ccaaggccgg tgccgcgccc
    10921 ctgctccttg gtggtgaaaa acggctccca gatcttgccg agaatatcgg ccggaatgcc
    10981 gccgccggtg tcctccacga tcagcgcggt gtaatcggcg atcggcagga tatcgctgcg
    11041 cagcgcccgc acatcgcccg ccgccacccg ccgcgtggcc agcaccagcc gccccgcgcc
    11101 atcgccgccc accttcgcgg aataatcctg catggcatcg cgcgcattga cgacaaggtt
    11161 gacgatgacc tgctccagct gcgtcggatc tgcccgcacc gggcccagct ggcgatcatg
    11221 atgcacttcc agcacgatct tctcgccgat cagccggcgc agcatctggc tgatttccga
    11281 aacgatatcg ggcagttgca gcacttcggg gcgcaaggtc tgctggcggc tgaaggccag
    11341 cagctggcgt gtcaggctgg ccgcgcggtt ggacgaggcg cggatctgct ggatatcgtc
    11401 ataatcgctg tcgcccggcg tgtggcgcag cagcatcaga tcgcaagtgc cgatgatggc
    11461 ggtgagcaca ttgttgaaat catgcgccac gccgccggcc agctggccca ccgcctgcat
    11521 cttggtggcc tgggccacct gccgcttcag ccgcgcctcc tcggtggtgt cgatcagcga
    11581 gagcagcacc gccgcctcgc ccagcccgcg cacgccggcc agcgacatcg tcaccggatc
    11641 ttccggctgg gcggccaggc gcaccgccac atcgcccgag atcaccgccc cttgcgcaaa
    11701 gcggcgcacc gcatcggcca gcgccgcctt gtcctcgcgc accacaaggt cgatcggcca
    11761 caccggcggc gcctcgccct catgcccggc cgcgcgcatg aaagcggcat tggcgaacag
    11821 cagccgcccg tcgcggtcgg ccatcgccag gcccagcggc agcggcgcca gcagcgcttc
    11881 cagatggggc gcggcgcccg cggtcatgcc ggcagcgccc accccctggc catgttccac
    11941 cagcagcatc agcgccggcg cctgctcgcc cccggccagc ggcaggcaat agacgatgag
    12001 cgggcgggtg cgatggctgt cgctggccca atagacgcgg gcgtgctcgt cctgcgtcag
    12061 cagcagggca aaatccgcct gcgtgccggc atggtggaga aaggcctcgt tggcggcatg
    12121 cacgcgcccc tcggggctga ccagcgcggc ggcaaagccg gcctcggcaa acagcgcgcc
    12181 ggcagcgcct gtcaccgcct gcgccacttg cgccagcgga tccacccgca ccaccgcgcc
    12241 cgcctgccag atgaggaaat cctccgcccg cccgcagcgc ttcaccgatg cctgccactg
    12301 gcgcccgctg ccatcgcgca gggccacagc gccggccgcg ccatcgcgca gggcctggcg
    12361 cgcggcctcc tgcatggccg cttgcgaagg gccatccagc cccggcgcca gtggcgcatg
    12421 ggccagcgag aaccagcttt cccacaggct gctggcacac accagccgcc cggcgcgatc
    12481 gactacggcc accgccgtat cgggccgctc catcgccgcc acggtcacgc tccagtcggg
    12541 ctgggccagc gcgggcaccg ggcgggcctg tgcgtccgcg cccagcttca gcgccagcgc
    12601 gcccagcacc gccaccccgc ccagaaagct gagcaccacc acgccttcgt gaaacaccag
    12661 ccacagcagc gccgtcacca cgatcagcgc ggccgcggcc ggccacatcg ccgcctggcc
    12721 atcgccggca gccgccagcc cggcgcgcag ccgcgccagc accgcgccca actccgccgc
    12781 cggccgcccg cccttgcccg gccccaaggc taggccctgc ccacagcgat gccgctgccc
    12841 agccgccgcc ttcgccgccg ggtgacgatg cagcgccaga tcacgctggt gagcacatag
    12901 cccagagccg ccaccacggt ggcttccacc agcaggccat aggccagcgc cagccccttg
    12961 ccggtcagcc ggtgcagcat ctcggtatga tcgccggcgc tcatcaggct cgacaccggc
    13021 tggcccgaca tcgccgcatc ggcatgcagc agaaacttgc ccaccttgta ggccaccggg
    13081 aaaatcagca cccaggtggc cggattgctg agcagcgtgg tggccaccgc caccggaata
    13141 ttggcccgca ccgccaccgc caccagcgcc gccacaaaac tgtgcgcgaa gggaatgaga
    13201 atgccgacaa acagccccag cgccaccccg cgcggcacgc tgcgccggtt gaaccgccac
    13261 aggcccggcc gcagcaccag atgggcaaag ggccgcagcc agcggttgct ctccagcttc
    13321 tcgcgggtgg gcacggcatg ggccagcagg cgcttcagcg cgcccgcctg ctgcccgccg
    13381 cgcgtcactt gtagtctctc aggatgcgct gctggtcgcg cttccagtcg cgctccttga
    13441 tagtggcgcg cttgtcggcc acattcttgc ccttggccag cgccagctcc accttggccc
    13501 gcccgcgccc gttgaaatag accgacagcg gcaccagcgt catgcccttg cgttccacag
    13561 cgccggtcag cttgtcgatc tcacgcgcgt ggagcagcag cttcctgggc cgcttgggct
    13621 cgtggttgaa gcggttgccg tgggaaaatt cgggcacgtt ggcgttgacc agccacacct
    13681 cgccgttctt cacctcggca taggcctcgg cgatggagcc ttcgccaaag cgcaggcttt
    13741 tcacttccgt gccggtaagg gcaatgcccg cctcgaaagt atcgtcgatg aaatagtcga
    13801 agcgcgcgcg ccggttttcg gcaacggttt tcttcttgtc gaacgtagcg ggagtcggac
    13861 gggccatgat gggggcgatg tagggcgctg caggccggaa cgaaagaggc gctgtcagat
    13921 aaaaccgccc agcccccgct tttttgaggc aggcagcaaa gcaagagccg ccccggcatg
    13981 aaaccggggc ggctctcaag ccctgtcagc gcaagctgac ggttttatga gcgccagcgc
    14041 gggctgacgg tattatcaag cctcagcgca ggctgacgac attgtcggcg cggcgcaggt
    14101 cgcggcggtc gcccagatac tggctggcca tcggctcgtc cgccgcctcg atggtgaggc
    14161 cggcgccaaa gccgttgaac ttggtgcgca tggccacgcc ataggcgccc agcatgccca
    14221 cctcgatata atcgcccgcc tcgatgccgg ccggcagggc aaagggccct tccatatagt
    14281 cggcatcgtc gcaagtgggg ccatagaagg cgaactcgcc cgccgggccg tccttttcca
    14341 cgccatcggc accgatcgcg cggacgggga agcgccagtc gacatgggcg gcatcataga
    14401 gcgcgccata ggccccctcg ttgatgaaca gctcgttgcc gcggcgcttt tccaccttga
    14461 cgatcatcga ggagaactcg gccgagaggg cccggcccgg ctcgcaccac agttccgacg
    14521 aataggacac cggcagcgat tcaaacgcgc ggtggatggc gccgaaataa tcctccagcg
    14581 gcggcggggt catgcccggg tagatcgacg ggaagccgcc acccacatcg atgatgtcca
    14641 ccgtgaccga ggcatcgacg atcgccgcgc gggcacgctc cagcgcctgc acataggcaa
    14701 agggcgtcat cgcctggctg cccacgtgga agcagatgcc caggcaatcc gccacctggc
    14761 gggtggcctg cagcaggggc gctgcatcca ccagatcgac accgaacttc gaggccaggc
    14821 tcagctccga atattcggag gacacccgca gccgcacgca cagcgacagg tcggtggccg
    14881 cattgccctc ggcatcgcgg gtcgcctcga cgatcttctc cagttcctca tgggtatcga
    14941 ggctgaaggt gcgcacgcca taggtgtgat aggccgcagc aatcgccgca aagggcttca
    15001 ccgggtgcat gaagcacagc acgggcgcat cgcccagcgt ctcgcgcacc agacgcacct
    15061 cggtgatcga ggcggtgtcg taatgcgtga tgccgccggc ccacagcacg cgcagaagct
    15121 cgggcgaggg attggccttg accgcataga ggctcttgcc cggaaacgtc tcggcgaaga
    15181 aacgggcagc ccgctccgcg gcgtgcggac ggttcaggat aacgggttcg ttaggcgcga
    15241 aatcgcgaac tacagccggt gcgtcgaggt gaatggacaa ctcaagggac ccccaatagg
    15301 ccttgcggcc agataaacca atggacgaag ccgccttgcg gtttggaagt ccccatgggg
    15361 cggcgagggg ggtgcatata gggcgatttc gggggggtgc aagataaaaa atacccccct
    15421 cgcccggtgc tggcgcagcc atggccgcgc atggcggcaa actggccctt atggcatttg
    15481 agtgacagtt taatgacgga aaaacgccgc ttgtgacagg cccgcagcgc ctatcccagc
    15541 cggtcgcgga aggtcgtcca atcgaatttc ttgacgcatt ccagcgaatc atccgctgaa
    15601 tcccagagcc agatcgaagg caaagggaca ccattgaagg tcgtcgtctt caccatggaa
    15661 taatgcgcct ggtcgagaaa ggcaaagcgc tggccgggct ctgccggcac cggcaggcgg
    15721 taatcgccga tgatgtcgcc agcaaggcaa gaggggccgc ccagccgcac ctcggggcct
    15781 tccgaaagct cgcccagcat ggccgggcga tagggcgcct cgatcacatc gggcatgtgg
    15841 caggtggccg aaatatcgac gatcgccacc ggcatgccat tctcatgcgc atccagaatg
    15901 ctgccgacga ggatgccggc atccagcgcc accgcctcgc ccggctccag atagagggtg
    15961 cagcccgtct cggcgcgcac gcggatgagg aagtccacca gctcgtcgcg ctgataatcg
    16021 gcgcgggtga tatggtggcc gccgccaaag ttcagccagc ggatcggccc gccattggcc
    16081 gccagccagg gagcgatgcg cggcgccagc gcttcccatg tgcgcttcag cggttcgaaa
    16141 tcctgctcgc acagcgtgtg aaagtgaagg cccgagacgc cctccagatg ttccgcgcgc
    16201 agctggctga ccggaaagcc cagccgcgag ccaagcgcgc aggggtcgta tttctccacc
    16261 tcgccctcgc ggtgcagcgg gttcagcctg aggccgacat cgaagctggc gccttccgcg
    16321 cgcgcgcgcg ccagttcggg cgcgaagcgg gccgcctgca tcggcgaatt gaagatcaca
    16381 tggtcggaaa tggcgcagat ctcggccatg tccgccgcct tgtagccggc gcaataagtg
    16441 gcaatctcgc cgcggtaata gtcgcgcgcc agccgcgctt cccacaggcc gctggtgcac
    16501 acgccatcga gataatcccc gatcagcggc gccaccttcc acatggaaaa ggccttcatc
    16561 gccgacaaca ccttcacgcc cgcccgcgcc ttgatatcgg cgagcacggc aaggttggcg
    16621 cgcagccggg cggcatccac cacgaaagcg gggctgtcca cccggccaag gtcgaaatgg
    16681 gcaaaggccc cgggatcgcc cgcgcgcgtt tccataagct gtcagttccc tttgaaaatc
    16741 aggcccgcgc cggcaatgat cagcgcaaaa ccggccagtt gcggccagcc cggcttctgc
    16801 ccgaacaaag cccaggccac caccacgaaa ccggccagcg aaaagacttc ctgcatggtt
    16861 ttcagctggg gcagcgaata ggccctggcg ccaatgcgat tggccggcac cgccaggcaa
    16921 tattcgcaaa aggccaggcc ccagctggcc agcacggcca cccagatcgc cttgtggggc
    16981 gccctgaggt ggccatacca tgccacattc atgaacaggt tcgatccggc cagcatcagc
    17041 ggcggcagga tcaaccccca gttcatcaaa aggccccgtt catcagaagg ccaccgggcc
    17101 ttccagctct tccaccgtcc agggcaggcc ctgggtgttg agcagcgcca tgaacggatc
    17161 gggatccatc tgctccatgt tgaacacgcc ctcgccatcc cacacgccct gcatgatcag
    17221 cgccgcgccg atcatcgccg gcacgcccgt ggtatagctg acggcctggt tgccggtttc
    17281 ctcgaaacag gcttcatggt cgcagatgtt cttgatgtaa aaggtcttct cgccgctgcc
    17341 atcaagcgcc tcgccggtgg cgatatcgcc aatgttggtc ttgcccttgg tagtggcgcc
    17401 cagcgtgctg ggctcgggca gcaccgcctt caggaactgc agcggcacga tctccacgcc
    17461 attgtagagc accgggtcga tccgcgtcat gcccacgttc tgcagcacgg tgaggtgctt
    17521 gatgtattca tcgccgaagg tcatccagaa gcggccgcgc tccagctccg gcaggaactt
    17581 ggcgaggctt tccagctcct cgtgatacat catgtacatg ttcttggggc ccacgccctc
    17641 gaagtcgaag ctcaccttct tcgccatcgg cggggtttcc acgaatgcgc cgccttccca
    17701 gtggcgggcc ggcgcggtca cttcgcggat gttgatttcc gggttgaagt tggtggcaaa
    17761 ggcctggcca tggtcgccgc cattgcaatc cagaatgtcg agctggcgga tggtcttcag
    17821 cttgtgcttc ttcagccaca tggcaaacac cgaggtaacg cccggatcaa agcccgagcc
    17881 cagcagcgcc atcagcccgg ccgccttgaa gcgctcctga taggcccact gccagtgata
    17941 ttcgaacctg gccacatcct tgggctcgta gttcgccgtg tcgagataat gggcgccggc
    18001 ggcaaggcag gcatccatga tcgtcagatc ctggtagggc agcgccaggt tcaccaccag
    18061 cttggggccg atctggcgga tcagcgcgct cagcgccggc acgtcctcgg catcgatggc
    18121 ataggtggcc accgtctcgc cggtgcgctg cttcacgctt tcggcaatgg catcgcactt
    18181 ggccttcgtg cggctggcca gatggatctc ggggaagatg gccgcgttca tcgccatctt
    18241 gtgcaccgca acgctgctga cgccgcccgc gccaatcacc agaaccttgc tcaccgcgct
    18301 caaactcctg ttcctggcgg cgctggccct gccgggcctc gccgctcgtc aagcgcgcca
    18361 tatagggcag gattgtgaca gggcaacagc acagcgccaa accacctgca aaccgcccct
    18421 tttgcccggc ccgcaccgcc cgccggcaaa taaaccgctc ctgtgacgca aaaccgtcac
    18481 cgttccgtca tgcaggcgca acagcgcagg cgtagcgccg atgggaacac ctccggcccc
    18541 ggccggcccg ttccattcgc gcgcagccgg ccccaccggc ccgccgcacc agatccgcag
    18601 cgagcctcga tgtctgccat ccccgccagc cccgaccttt cctccggcca gcttcgcgcg
    18661 ggccatccca aggccgcccg cgtgatcgaa caggcggtgg tccgccggca tgtgcaggcc
    18721 aaggaacgcc tgctcgacaa ggccttcgcg ctcgccttcc gaggcctggt ctatgcccag
    18781 atctgggaag atccggtgat cgacatggag gcgctggaca tccagcccga caaccgcatc
    18841 gtgacgatcg ccagcggcgg ctgcaatgcg ctgtcctatc tcacggcgaa cccggcgaag
    18901 atcaccgcgg tcgatctcaa caccgcccat gtcgcgctca acaagctgaa gttcgccgcc
    18961 gtgcgccagc tgcccgatta taccgcgctg cgccgcttct ttgtagaggc ggacaacaag
    19021 gccaatgtcg gcgcctatcg caagtatctg cgcccgcacc ttgatgaagc cacccgccgc
    19081 tattgggaag ggcgcgacct gtttggccgc cgccgcatcc tgggctttgc caagggcatc
    19141 tacaagcgcg gcctgctggg cagcttcatc ggcatggcgc atcttgtcgc caaagcctat
    19201 cgcgttgatc cgcgcgtgat cctggaggcg cgcacgctgg aagaacagcg ccgcgtgttc
    19261 gacgagaaat tcgcccccgt gttcgacaag cgcttcatcc gctggctcac cagccagccg
    19321 gcctcgctgt ttggcctggg cattccgccg gcccagtatg acgcgctggc gggcgaccag
    19381 cgcatggccc atgtgctgcg cgcccggctg gaaaagctgg cctgcgactt ttccatccac
    19441 gacaattact tcgcctggca ggcctttggc cgcggctatg gcaaggcgga aagcgcgccg
    19501 ctgccgccct atctgcaggc gcagaactac gacgcactgc gcgagcggat cgaccgcatc
    19561 gaggtgctgc actgcaattt caccgaatat cttgccagcc agcgcgatgc cagcctcgac
    19621 cgctacatcc tgctcgacgc gcaggactgg atgagcgacc tgcagctgac cttcctgtgg
    19681 cgcgagatca cccgcaccgc ccgcccgggc gcgcgcgtgc tgttccgcac cgcagccgag
    19741 gaaaccctgc tgcccggccg cgtgcccgcc gaaatcctcg accgctggga ttattcggag
    19801 gccgaggccc agcgcctcat ccgcgccgat cgctcgtcga tctatggcgg cctgcacctc
    19861 tatcgcttca agggctgaac gccatggcca gcatcgcgcc tgccaccggc gggcatggcc
    19921 agctgatgga caatgtctat cgctggcagc gccacatcta tgatgccacg cgcaaatatt
    19981 tcctcttcgg gcgcgatcgg ctgattgccg ggctggatct ggccgagggc ggcacgctgc
    20041 tggaactggg ctgcggcacc ggccgcaacc tcgatctggc ggcccggcac tggccggccg
    20101 ccagcctcca cgggctcgac atttcggccg agatgctgaa atcggccgaa gccaagctgg
    20161 gcgcccgcgc gcagctggcc gaaggcgatg cctgcgcggt ggaagcagac gcgctgtttg
    20221 gccgcgcccg cttcgaccgg gtgatgatct cctttgccct ctcgatgatc cccgcctggg
    20281 aagcggcgct ggccgaaggg ctgcgcctgc tggcgccggg cggcagcctc cacatcgtcg
    20341 atttcggcga tgcggcgggc ctgccggcgc ctttgcgggc gctgctcaat ggctggctgg
    20401 cgcgtttcca tgtcagcccc cgcctcaccc tggccgatcg cgccacggcg ctggccgcca
    20461 ccggccgctt caaggtgcgc acccggcgcg ggcctttcgg ctattaccag ctgatcgtgc
    20521 tgacccggcg cagctagccg cgcacgaaca ggctggccag ttccacatgg gtgctccagc
    20581 ggaactggcc gaccgggcgc agttccacca gccgatagcc cgccgcctgc agttgcgcgg
    20641 catcgcgcgc ccagctggcc gggttgcagc tgacatagac caccctggcc accgtgctgg
    20701 ccacgatgcg ccccacctgt tcgcgcgcgc cggcccgcgg cggatcgagc accaccgcgc
    20761 caaagcggtt cagctcttcc ggcagcagcg ggttgcggaa cagatcgcgg tgaagcgcgg
    20821 ccaccggcag cagcgcgcgc gccgccgccc ccttgcaggc cagatgcgcg gcccggtcgg
    20881 cctctgccgc cagcaccttg tggccggctt tcgccagcgc cagcgcaaac gtgcccagcc
    20941 ccgagaacag atcggccacc acgccgctgc cctccagcca ggccaccgcc gcgcccacca
    21001 gcgccgcctc gccatcggcc gtcgcctgca ggaaggcgcc cggcggcagc ggcacggcaa
    21061 tctcgcccag cgtgatcgtc acgccatcgg gctcccacag gctttccggc ccatagccct
    21121 gatcgagcga caggcgcgcc agctgctgtt cgcgcgcaaa ggccagccac gcctcggtcc
    21181 gcgccagccc ttccaccgtc aggcctttaa gggccagatc cacgccctga tcggccagcg
    21241 tcatgtcgat ctctacagac aggcggccat cctgccgggc cagcatgcgc cgcaaggggg
    21301 cgatcagcgc caccagctcg ggcgccagca ccgggcattc gccaatatcc accagccggt
    21361 gcgattgccc ctcgcggaag cccagcgcca ccgcgccgcc cacgcgggcg gcatgcagcg
    21421 ccgcccgccg ccggctgccc cgcggcgaca gatgcaccgg cgccagcaca tcgggcacca
    21481 gcctctggcc agccgccgcg cccgccaccc gctcgcccac gaaggtggcc agggcggcat
    21541 cgtccacatg ctgcagctgg cagccgccgc attggccata gtggcggcag gccggctcgg
    21601 catgatgggg ccccttgcgc aaggccccat cggccagcac cacatcgccg ggcgccgcgc
    21661 cggccacatg gcggccgctg gcagtggcgc cgtcgccgcg cgcggcgatg cggatgatca
    21721 tctcttccat gcaaactccg aaagctagag cgcggtgcga taggctgccc gcaccattcc
    21781 ggcaagctgc ccggcggtga aaggcccgcc agccaggcgc gcggcctcgc catgcagcca
    21841 cagggcggtg gcggcggcgg caaaagcgtc ttcgccggcg gccagccggg ccgccacgca
    21901 gccggccagc acatcgccgc tgcccgccac cgacagccag ctgggcgccc gcgccgcaca
    21961 cagcagccgg ccatcgggcg ccgcgatcag cgaatccggc cccttggcaa ggatgaccag
    22021 cccgctagcc tgcgccaacg cctgtgcccg ctgccacttg gcgccagcgc cggaaaggcc
    22081 aaaggcccgc tccatggccg ccagctcacc ctcatgcggc gtggcgatgg cgccttccgg
    22141 cagcgcatcg
//
LOCUS       DASRYE010000163         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_9271,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000163 DASRYE010000000
VERSION     DASRYE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9271"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="VFF98_08570"
     CDS             <1..2415
                     /locus_tag="VFF98_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63724.1"
                     /translation="RGLNSEYNVNLINGVNVAQGMPYSRQVQLSLLPPSGLNTIVVEK
                     ASTAEMDGDAIGGTVDFRTPTAYDFAKPFHAAFSMSGRMETRDQDYGNAGLGGGFSGE
                     ISKRFGGNDEFGVYVSGYYDVRYFTNSELGGVMAAQNDGGWGYIMAANASGSAPAAGL
                     DPQKDITQTGIDVGSSSGYTARWGTNASFDWHPDADTQVYLRGTYASAKTQQNSTLGE
                     YVSDSKSWNQVGTSSNYALAVNTISTRVWYETNPELANLATAELGLKKKLGKWTITPQ
                     IFYSEGHNDRPNHIEASERVNQKDNYNSGQSLALGGQSIVYVNGLPQPVMTSAIYNSL
                     NNANTQTLARRAGQLTEEFSGQNKYGARFDVARDIDGSILQQIRFGAKYAMSHRLFTE
                     RDWTNNQFANLLGTGGATWQSLGISTSYYNQAFPGVYTFQLPKVNQAALLNYFYKYQS
                     ASSLDTCGGTATDNWNCDTMRGSEAVSAFYLAGTIKAGNLEVIPGLRYEHTAIHNTYW
                     QIGDGDTVASGWASNRTHYNEWLPSLFANYRPNANAVYRADLWWSYTRPAFVQLGGGS
                     TVSVGDGVTTITQGNPNLKPIQAMNLDVSGQWKFGPETLLSAGGYYKHLRHYMYDNGS
                     GYINSGTLAENTTIYNVPQNGGSGNVYGLEAQMRTRFTGLPAWFGKLGVNLNATHQWT
                     KVDIGGGVMKKIQNAPNWLANAQLFWEKGPASVDLTFKYTGEYVSGYSALGIGTWDDL
                     WVRPLKTVDAHVGYKLRQDLRADFSVANVFGAYSYWSHIGHDTLALSDVVDSGRTLLL
                     TLKWSR"
     gene            2494..>3041
                     /locus_tag="VFF98_08575"
     CDS             2494..>3041
                     /locus_tag="VFF98_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HZU63725.1"
                     /translation="MRLFCLFLSGLLALAPGLADARRQPSPVPGPLHAGTRVERLVLL
                     YRHGVRAPLAGEAGMAPLASAAMPGWVTPESLLTPHGAAAIRLLARYQGGLWRAAGLL
                     PATGCPTAADLAIWTNTPARTIATGEALAAGLAPGCAVAVGHLPAGRHDPVFEQREAG
                     GDPFNAAQAARDVNRSTGGAAA"
BASE COUNT          560 a         1033 c          962 g          486 t
ORIGIN      
        1 cgcgggctca attcggaata taacgtcaac ctcatcaacg gggtgaacgt ggcgcagggc
       61 atgccctaca gccgccaggt gcaattgagc ctgctgcccc cttcgggcct caacaccatc
      121 gtggtggaaa aggcctccac cgccgagatg gatggcgatg cgatcggcgg cacggtcgat
      181 tttcgcaccc ccaccgccta tgatttcgcc aagcccttcc atgccgcctt ctcgatgtcg
      241 ggccggatgg aaacgcgcga tcaggattat ggcaatgccg gccttggcgg cggcttttcc
      301 ggcgagattt ccaagcgctt tggcggcaac gacgaattcg gcgtctatgt ctcgggctat
      361 tacgacgtgc gctatttcac caacagcgag ctgggcgggg tgatggccgc gcagaacgat
      421 ggcggctggg gctatatcat ggccgccaat gccagcggca gcgcgccggc tgccggcctt
      481 gatccgcaaa aggatatcac ccagaccggc atcgatgtcg gctcctccag cggctatacc
      541 gcgcgctggg gcaccaatgc ctcgttcgac tggcacccgg atgccgatac gcaggtctat
      601 ctgcgcggca cctatgcctc tgccaagacc cagcagaatt cgacgctggg cgaatatgtc
      661 tccgacagca aatcctggaa ccaggtgggc accagcagca attacgccct ggccgtcaac
      721 accatttcca cccgcgtgtg gtatgaaacc aacccggaac tggccaatct ggccaccgcc
      781 gagctggggc tgaagaagaa gctgggcaag tggacgatca ccccgcagat cttctattcc
      841 gaaggccata acgaccggcc caaccacatc gaggcttccg agcgcgtcaa ccagaaggac
      901 aattacaaca gcggccagag cctggcgctg ggcggccagt cgatcgtcta tgtgaacggc
      961 ctgccgcagc cggtgatgac cagcgccatc tacaattcgc tgaacaacgc caacacccag
     1021 accctggccc gccgcgccgg ccagctgacc gaggagttca gcggccagaa caaatatggc
     1081 gcccgcttcg atgtggcccg cgatatcgat ggctcgatcc tgcagcagat ccgcttcggg
     1141 gccaaatatg cgatgagcca ccgcctgttc accgagcgcg actggaccaa caaccagttt
     1201 gccaacctgc tgggcacggg cggcgccacc tggcaatcgc tgggcatttc caccagctac
     1261 tacaaccagg ccttccccgg cgtatacacc ttccagctgc ccaaggtgaa ccaggccgcg
     1321 ctgctcaact atttctacaa ataccagagc gccagcagcc tcgacacctg cggcggcacc
     1381 gcaaccgaca actggaactg cgacaccatg cgcggcagcg aggcggtgag cgccttctac
     1441 ctcgccggca ccatcaaggc cggcaacctc gaggtgatcc cgggcctgcg ctatgagcac
     1501 accgccatcc acaacaccta ctggcagatc ggcgatggcg acacggtggc cagcggctgg
     1561 gccagcaacc gcacccatta caacgaatgg ctgcccagcc ttttcgccaa ctaccggccc
     1621 aatgccaatg ccgtctaccg ggcggatctg tggtggtcct acacgcggcc ggcctttgtg
     1681 caactgggcg gcggctccac ggtgagcgtg ggcgacgggg tgacgacgat cacccagggc
     1741 aaccccaacc tcaagccgat ccaggcgatg aacctggatg tgtcgggcca gtggaagttc
     1801 ggccccgaga cgctgctgtc ggccggcggc tactacaagc acctgcgcca ctacatgtat
     1861 gacaatggca gcggctatat caacagcggc acgctggccg agaacaccac catctacaat
     1921 gtgccgcaga acggtggctc gggcaatgtc tatgggctgg aagcgcagat gcgcacccgc
     1981 ttcaccggcc tgccggcctg gttcggcaag ctgggggtga acctcaacgc cacccaccag
     2041 tggacgaagg tcgacatcgg cgggggcgtg atgaagaaga tccagaacgc gcccaactgg
     2101 ctggccaatg cccagctgtt ctgggaaaag ggcccggcga gcgtggatct caccttcaaa
     2161 tacaccggcg aatatgtctc gggctacagc gcgctgggca tcggcacgtg ggatgatctg
     2221 tgggtgcgcc cgctcaagac ggtggacgcc catgtcggct acaagctgcg ccaggatctg
     2281 cgggcggatt tctcggtggc caatgtgttc ggcgcctaca gctactggtc gcatatcggc
     2341 cacgacacgc tggcactgtc ggatgtggtc gattcgggcc gcaccctgct gctgacgctg
     2401 aagtggagcc gctgaccggc tgatcgcgca aagggggcgc ggccggtggc ggcgccccct
     2461 tctgttgcag cctgtgctat ccggggagcg ccgatgcgcc tgttctgcct gtttctgtcc
     2521 ggcctgctgg cgttggcgcc gggccttgcc gatgcccgcc gccagccttc ccccgtaccc
     2581 ggcccgctgc acgccggcac gcgggtggaa cggctggtgc tgctctatcg ccatggtgtg
     2641 cgtgcgccgc tggcgggcga ggcgggcatg gcgcctctgg ccagcgcggc gatgcccggc
     2701 tgggtcacgc cggaaagcct gctcaccccc catggcgcgg cggcgatccg gctgctggcg
     2761 cgctatcagg gcgggctgtg gcgggcggcg gggctgctgc cggccaccgg ctgccccacc
     2821 gcggcagacc ttgccatctg gaccaacacg cccgcccgca ccatcgccac cggcgaggcg
     2881 ctggccgcag ggctggcgcc gggctgcgcg gtggcggtgg gccatctgcc cgccggccgc
     2941 cacgaccccg tgttcgagca gcgcgaggca ggcggtgatc ccttcaacgc cgcacaggcc
     3001 gcgcgcgatg tgaaccgcag caccggcggg gcggcggcca t
//
LOCUS       DASRYE010000164         8563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_40651,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000164 DASRYE010000000
VERSION     DASRYE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40651"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF98_08580"
     CDS             complement(<1..255)
                     /locus_tag="VFF98_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63726.1"
                     /translation="MSDKPEPPRPDYTSIGAYVSGGLARAGSAARATLANIPWDQPAT
                     MIDLDGRTPIIGTIRECAQHFGLYKPEARAQARIVLTKPVH"
     gene            complement(248..2800)
                     /gene="gyrB"
                     /locus_tag="VFF98_08585"
     CDS             complement(248..2800)
                     /gene="gyrB"
                     /locus_tag="VFF98_08585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZU63727.1"
                     /translation="MSSDTENTPSHSATSSHYGADSIKVLKGLDAVRKRPGMYIGDTD
                     DGSGLHHMVFEVSDNAIDEALAGHCDLVLIELNPDGSVSVEDNGRGIPTDIHPEEGVS
                     AAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWREGQEHWM
                     KFAHGDAVAPLVPRGPAPLVERGSRKGDPKKGTRVTFFASHDTFKNVTEFDFEKLEHR
                     YRELAFLNSGVRILLRDRRHEQVKEHDLYYEGGIAAFVKWLDRNKIALIPEPIAISAD
                     RDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYAERSGLLK
                     KEKVSLSGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADKMSEWLE
                     ENPAHAKAIIQKVIDAAAAREAARRARELTRRKGVMDIASLPGKLADCQERDPSKCEL
                     FLVEGDSAGGSAKQGRDRTVQAILPLKGKILNVERARFDRIISSKEVGTLIQAMGTGI
                     RDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPDIIRAGHLFIAQPPLYKV
                     AKGRSEVYLKDNAALDRYLVEAGLAGRLLETAGGARSGPDLESLVDHALRLRNLMAFV
                     PRKYNEAVVEAMALAGALEHGLTPAERGEALDKAAQWLGRADREAKWSAGLAEEGHAE
                     FFRVWRGVTDYHKIDAAFLISAEARKLARIAAEQAEVYATPARLVRSTAQAAEPEPEA
                     PEAESEEAAPVAARPVDTEGAISRPSQLLDAVLATGRKGLSIQRYKGLGEMNAEQLWE
                     TTLDPNNRALLQVKVEDADVTDEIFTRLMGDIVEPRREFIQENALNVANLDV"
     gene            3672..5264
                     /gene="guaA"
                     /locus_tag="VFF98_08590"
     CDS             3672..5264
                     /gene="guaA"
                     /locus_tag="VFF98_08590"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZU63728.1"
                     /translation="MTAQERNTPAESSVHHAESILIVDFGSQVTQLIARRVREAGVYS
                     EIAPFTLAEAAFHRMAPKGIILSGSPASVLDENGPRIPAAILESGLPMLGICYGQQAL
                     MHQLGGEVSRGDAGEFGRAFIEIADKCALFDGLWAEGETHQVWMSHGDKVTALAPGFR
                     PVAASPGAPYAVIANDDKRIYAMQFHPEVVHTPDGGKLLKNFVRHVCGLAGDWTMAEF
                     RKTKIAEIRAQVGDRRVICGLSGGVDSAVAALLIHEAIGDQLTCVFVDGGILRMGEAE
                     QVVSLFRGHFNIPLVHVDASTLFLNGLAGVTDPEAKRKFIGKTFIDVFEDEAKKIGGA
                     DFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRAL
                     GRELGLPEIFVGRHPFPGPGLAIRIPGEVTRERCDILRKADAIYLEEIRNAGLYDAIW
                     QAFAVLLPVKTVGVMGDGRTYDSVCALRAVTSTDGMTADIYPFDAAFLSRVATRIINE
                     VKGINRVVYDYTSKPPGTIEWE"
     gene            5450..6472
                     /locus_tag="VFF98_08595"
     CDS             5450..6472
                     /locus_tag="VFF98_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU63729.1"
                     /translation="MTESRHSKVTAQDVARKAGVSQSAVSRAFTPDASVSPVTRQKVL
                     KAAAELGYRPNAFARSLTTRRSDIIGVAMGNLQNPFFAAALEEITHQFARAGLRLLVF
                     SARPDMPVEEIMQYQIEALLMLSAPLSPRLKSECQVGRVPVVMFNRSSLEPDISSVVG
                     DNAQGAATIAAYLLAGDHRRIAYMALGQENESTASVEREAAFTAYLAAQGHRLHSRAA
                     ADFTSAGAAAAMRKLLLQPERPDAVFCANDHLAIMALQVAQAEFGLEVGRDISIVGFD
                     DVPQASWPGISLTTFSQPIAPMVSAALDLIEDLRSGEAPPQQVVIPGDLVVRSSARRP
                     RYTAGG"
     gene            complement(6469..6879)
                     /locus_tag="VFF98_08600"
     CDS             complement(6469..6879)
                     /locus_tag="VFF98_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63730.1"
                     /translation="MKPAIIGALAVMLAAPAMAADDLPLPVARTLGNSLHYQAPDTHF
                     AMVIRDSADHRYEAVVDLRDPAGRPVHLRMSGRWTIEGDRICRQQTSPPPPPGHDRIC
                     EAYGAYPAPGESRTDPATGLILTLVLTPAPGSAP"
     gene            complement(6876..7397)
                     /locus_tag="VFF98_08605"
     CDS             complement(6876..7397)
                     /locus_tag="VFF98_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63731.1"
                     /translation="MTNLAYLADRLAIEDVVLRYAWASDTLDADLYLSVFAADARITL
                     PNGTILAAGHDQIGATARTNQIRFLAPAGGLSVMRHLVANLAVDITGDAAVARYCVTT
                     LGFDTQAAKPEIVSLARYVDRLARHGAGWLIAERTMHYDWGNDAMARQLAIGPHTPPQ
                     YRPAPRPSPEQGQ"
     gene            complement(7407..>8563)
                     /locus_tag="VFF98_08610"
     CDS             complement(7407..>8563)
                     /locus_tag="VFF98_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63732.1"
                     /translation="AMMLGLIPQGLAEAAITASLPDMAHDLGRHGELAAQMMLGMSAL
                     GLVMGALASGRILEWAGTRRAFLGALLAFALGGGVGVATGDLALLYAGRVVVGFAASC
                     IATTCLWRINARYTGDGRAKFFGGAGATGGIATILSVLAGGALTQHFGWRMCLLIYPG
                     FAALALPIVLAGMDQQRPLPRRPRADGASTGYLAALLPLYGLVLLFYGLIGMASMQLP
                     FLLELNGAHGAGDRALIQGLPGLTSIAGASFFGLVQHRFGATRSFTASLGLFVAGLLL
                     FATARTGLVLPAIGAGAIGLCMGLSGPYFYHIIAQRTEVALAGRYLGYLNAFTFLGVF
                     ASPMVTAAIKPHTGLAGLFVGGAAMVAGMALVIGRASAGPAPRALSTPLA"
BASE COUNT         1402 a         2779 c         2947 g         1435 t
ORIGIN      
        1 gtggacgggc ttggtgagga cgatgcgcgc ctgggcccgc gcctcgggct tgtagaggcc
       61 gaaatgctgc gcgcattcgc ggatcgtgcc gatgatcggc gtgcgaccat cgaggtcgat
      121 catcgtggcc ggctgatccc aggggatatt ggcaagggtg gcccgggcgg cgctgcccgc
      181 gcgggccagc ccgccgctca cataggcgcc gatcgaagta tagtccggtc ggggcggttc
      241 gggcttgtca gacatcgaga ttggccacgt tgagcgcgtt ttcctggatg aattcgcggc
      301 gcggttccac gatatcgccc atcaggcgag tgaagatctc gtcggtcacg tcggcatcct
      361 ccaccttcac ctgcagcagg gcgcggttgt tggggtcgag cgtggtttcc cacagctgct
      421 cggcgttcat ctcgccaagg cccttgtagc gctggatgga aaggcccttg cggccggtgg
      481 ccagcaccgc atccagcagc tggctgggcc ggctgatggc gccctcggta tcgaccgggc
      541 gggcggcaac gggggcggct tcctcgctct cggcctcggg ggcttcaggc tcgggctctg
      601 cggcctgcgc ggtggagcgc acgaggcgcg cgggcgtggc atagacctcg gcctgctcgg
      661 cggcgatgcg ggccagcttc ctggcctcgg cgctgatcag gaaggcggcg tcgatcttgt
      721 ggtaatcggt cacgccgcgc cacacgcgga agaattcggc atggccttcc tcggcaaggc
      781 cggccgacca tttcgcttcg cggtcggccc ggcccagcca ttgggcggcc ttgtccagcg
      841 cctcgccgcg ttcggccggt gtcagcccat gctccagcgc gccggccagc gccatggctt
      901 ccaccaccgc ctcgttatac ttgcgcggca cgaaggccat gaggttgcgc aggcgcagcg
      961 catgatccac caggctttcc agatccgggc cggaacgcgc gccgcccgcc gtttccagca
     1021 ggcgcccggc aaggccggct tccaccagat agcgatcaag cgcggcattg tccttgaggt
     1081 agacctcaga gcggcccttg gccaccttgt agagcggcgg ctgggcgatg aacaggtgcc
     1141 cggcgcggat gatatccggc atctggcggt ggaagaaggt cagcagcagg gtgcggatat
     1201 gggcgccgtc gacgtcggcg tcggtcatga tgacgatctt gtggtagcgc agcttctcaa
     1261 ggttgaactc gtcgcggatg ccggtgccca tcgcctggat gagggtgccc acttccttcg
     1321 acgagatgat ccggtcgaag cgggcgcgct ccacattgag gatcttgccc ttcagcggca
     1381 ggatcgcctg gacggtgcga tcgcggccct gcttggcgct gccgccggcc gaatcaccct
     1441 cgaccaggaa cagttcgcat ttgctgggat cgcgctcctg gcaatcggcc agcttgccgg
     1501 gcaggctggc gatatccatc acccccttgc ggcgggtcag ctcgcgggcg cggcgggcgg
     1561 cctcgcgggc ggcggcagca tcgatcacct tctggatgat ggccttggcg tgggcggggt
     1621 tttcctccag ccattcgctc atcttgtcgg ccatcaggct ttccagcggc tggcgcactt
     1681 cggaggagac cagcttgtcc ttagtctggc tggagaattt gggatcgggc agcttcaccg
     1741 agacgatcgc ggtgaggccc tcgcgcatgt cctcgcccga cagcgagacc ttttccttct
     1801 tcagcaggcc cgaacgctcg gcatagccgt tcagcgtgcg ggtgagcgcg gcgcggaagg
     1861 cggcaagatg ggtgccgcca tcgcgctggg ggatgttgtt ggtgaaacac agcacgtttt
     1921 cgtaatagct gtcgttccat tccagcgcga catcgatgcc gatgccatcc cggtcggccg
     1981 aaatggcgat gggctcgggg atcagggcga tcttgttgcg atccagccac ttcacgaagg
     2041 ccgcgatgcc gccttcgtag tagaggtcgt gctccttcac ctgctcgtgg cggcggtcgc
     2101 gcagcaggat gcgcacgccc gaattgagga aggcaagctc gcggtagcgg tgctccagct
     2161 tctcgaaatc gaattcggtg acgttcttga acgtgtcgtg gctggcaaag aaggtgacgc
     2221 gcgtgccctt cttgggatcg cccttgcggc tgccgcgctc caccaggggg gcagggccgc
     2281 ggggcaccag cggcgccacg gcatcgccat gggcaaattt catccagtgt tcctggccct
     2341 cgcgccagat cgtcagctcc agccattccg acagggcgtt caccaccgaa acgcccacgc
     2401 catgcaggcc gcccgacacc ttgtaggcat tgtcgtccga ggtgttctcg aacttgccgc
     2461 cggcatgcag ctgggtcatg atcacctcgg cggccgaaac gccttcttcc gggtgaatgt
     2521 cggtggggat gccgcggccg ttatcctcca cggaaacaga accatcgggg ttgagctcga
     2581 tcagcaccag atcgcaatgg ccggccagcg cctcgtcgat ggcattgtcc gacacctcga
     2641 acaccatgtg atgcaggccc gagccatcgt cggtatcgcc gatatacatg ccgggccgct
     2701 tgcgcacggc atccagccct ttcagcacct tgatcgaatc ggcaccgtag tggctgctgg
     2761 tggcgctgtg cgagggggtg ttttcggtat cgctgctcat gccaagccta tacgcattgg
     2821 cggccggcga tgaaacccgt ttcggcgttt tccgtccaca gggaaatcgc gggggtttgc
     2881 ggcggggaat gcggggaggg gcgagctggt cccgggccca gcgccggctg aagacgcagg
     2941 gcccggaaaa agaccagtcg ttgtgctgcc tgcccggatc aggatccatg ccgggcaagg
     3001 cggccttggg gccgcctgca ccttgcagtc acggcccttg gcgggcgcgc tgctgcctgc
     3061 cgcgccgggg tgcatgaggg gcgcgaaaag gcgggtgagg gcggtttttt cgggccgggg
     3121 gcgggcagat cggcgcttgc ctggcttcct tggcgcggct gaggaccagt cgtccacgtc
     3181 gggaggggca agaggccggc ctgtctgtcc ggggctcggg ggttgcgctt gccgggctga
     3241 ttgaaccgga aagcggcagg gagtgagggc ggcttgggcc ctgataggct tggtattaag
     3301 gctggtctgg aaaaatcagg ccagggtgac ggcgaaaaac agcccgctgg tgcccagcgc
     3361 gacagcgacc gccacgaggt tacgtgcgaa gtcgttcatg atgttagctc ctgcgaaaac
     3421 agcccttcag attgtcgctg caatactgca gtcgcgaagg tgtctgtccg agcggccata
     3481 tagcgatttt ttgcggaaat gctcgtgcga aaggggcaat gacgattgcg ttttgcgcaa
     3541 gttggtacat atatcggggc gttgctgcac tgcggtataa gcagggtgcc ggggccgccc
     3601 cggacccttg cgcgcgccgc cccttcgcgc gcgggctgga cacctgcgac ttgccagcct
     3661 aacgcaccgg catgaccgca caagagcgca atacgcccgc cgaatcgtcc gtccaccatg
     3721 ccgaatcgat cctcatcgtc gatttcggca gccaggtgac ccagctgatc gcgcgccgcg
     3781 tgcgcgaggc gggcgtctat tccgaaatcg ctcccttcac cctggccgaa gccgccttcc
     3841 accgcatggc gcccaagggc atcatcctgt cgggcagccc ggcttccgtg ctggatgaga
     3901 acggcccgcg catcccggcc gcgatcctgg aatcgggcct gccgatgctg ggcatctgct
     3961 atggccagca ggcgctgatg caccagctgg gcggcgaggt gagccggggc gatgccggcg
     4021 aatttggccg cgccttcatc gagatcgccg acaaatgcgc cctgttcgac ggcctgtggg
     4081 ccgagggcga gacgcatcag gtgtggatga gccatggcga caaggtgacc gcccttgccc
     4141 ccggcttccg gcccgtggcc gccagccccg gcgcgcccta tgccgttatc gccaacgacg
     4201 acaagcgcat ctatgccatg cagttccacc ccgaggtggt gcacacgccc gatggcggca
     4261 agctgctgaa gaatttcgtg cgccatgtct gcggcctggc cggcgactgg acgatggccg
     4321 aattccgcaa gaccaagatc gccgagatcc gcgcccaggt gggcgacagg cgggtgatct
     4381 gcggcctgtc gggcggggtt gattcggcgg tggcggcgct gctcatccac gaggcgatcg
     4441 gcgaccagct gacctgcgtc ttcgtcgatg gcggcatcct gcgcatgggc gaggccgagc
     4501 aggttgtcag cctgttccgc ggccatttca acattccgct ggtgcatgtg gatgccagca
     4561 cgctgttcct caatggcctt gccggcgtga ccgacccgga agccaagcgc aagttcatcg
     4621 gcaagacctt catcgacgtg ttcgaggatg aggcgaagaa gatcggcggc gccgatttcc
     4681 tggcgcaggg cacgctctat cccgatgtga tcgagagcgt ctccttcacc ggcggcccgt
     4741 cggtgacgat caagagccac cacaacgtgg gcggcctgcc cgagcgcatg aacatgcagc
     4801 tggtggagcc cttgcgcgag ctgttcaagg atgaagtgcg cgcgctgggc cgcgagctgg
     4861 gcctgccgga gatcttcgtg ggccgccacc ccttcccggg gccgggcctg gccatccgca
     4921 tccccggcga ggtgacgcgc gagcgctgcg atatcctgcg caaggccgat gcgatctatc
     4981 tcgaagagat ccgcaatgcc ggcctttatg atgccatctg gcaggccttt gccgtgctgc
     5041 tgccggtgaa gacggtgggc gtgatgggcg acggtcgcac ttacgacagc gtgtgcgccc
     5101 tgcgcgccgt gaccagcacc gacggcatga ccgccgacat ctaccccttc gatgccgcct
     5161 tcctcagccg cgtggccacc cgcatcatca acgaggtgaa gggcatcaac cgcgtggtct
     5221 atgactacac cagcaagccg cccggcacga tcgagtggga gtgagggata caagcggcgg
     5281 cccttttccg ctgcacatat ccagatcgtt gggcgcccgc cgcgatgccg gcgggcgcgc
     5341 cctcctgggc ttgaatgaaa aatttgcata acattgcata aattgcacgc atgctatagc
     5401 catgccgcca tgcgccaccg acgcccgcgc gccaggaaac aaggcagtca tgacggaaag
     5461 caggcacagc aaggttacgg cgcaggatgt ggcgcgcaag gccggggttt cgcaatcggc
     5521 ggtatcgcgg gccttcacgc ccgatgccag cgtctcgccg gtcacgcggc aaaaggtgct
     5581 gaaggccgcc gcggagctgg gctatcgccc caacgccttc gcccgttcgc tgaccacgcg
     5641 gcgttccgac atcatcgggg tggcaatggg caatctgcag aacccctttt tcgccgccgc
     5701 gcttgaagag atcacccatc aattcgcccg ggcggggctg cggctgctgg tgttctcggc
     5761 gcggcccgac atgccggtcg aagaaatcat gcaatatcag atcgaggcgt tgctgatgct
     5821 gtcggcgccg ctgtcgccgc ggcttaaaag cgaatgccag gtggggcgtg tgccggtggt
     5881 gatgttcaac cgttccagcc ttgagcccga catctcctcg gtagtgggcg acaatgcgca
     5941 aggggcggcc accatcgcgg cctatctgct ggccggcgat caccgccgca tcgcctatat
     6001 ggcgctgggt caggagaatg aatcgaccgc cagtgtcgag cgcgaggccg ctttcaccgc
     6061 ttatctggcc gcgcagggcc atcggctcca ttcccgcgcc gcggccgatt tcaccagcgc
     6121 cggcgcggcc gcggctatgc gcaagctgct gctgcagccc gaacggcccg atgcggtgtt
     6181 ctgcgccaat gatcatctgg cgatcatggc cttgcaggtg gcacaggccg aattcgggct
     6241 ggaagtgggg cgcgacattt cgatcgtcgg cttcgacgat gtgccgcagg ccagctggcc
     6301 gggtatttcg ctgaccactt tttcgcagcc gatcgcgccg atggtctcgg ccgcgcttga
     6361 cctcatcgag gacctgcgca gcggcgaggc gccgccgcaa caggtggtca ttcccggcga
     6421 tctggtggtc cgctccagcg cccgccggcc gcgctacacc gcgggcggct agggggcgct
     6481 gccgggcgcc ggcgtcagga ccagcgtcag gatcaggccg gtggccggat cggtgcggct
     6541 ttcgccgggc gccggataag ccccataggc ctcgcagatc ctgtcgtggc ccggcggcgg
     6601 tggcgggctt gtctgctggc ggcagatcct gtcgccctcg atcgtccatc gcccggacat
     6661 gcgcagatgg accggccggc ctgccggatc gcgcagatcc accaccgcct cgtagcgatg
     6721 atcggcgctg tcgcggatga ccatggcaaa atgggtgtcg ggcgcctgat agtgcaggct
     6781 gttgcccaaa gtgcgggcca ccggcagcgg caggtcgtct gccgccatgg cgggggcggc
     6841 cagcatcacg gccaaggcgc cgatgatcgc cggcttcact ggccctgctc cgggctgggg
     6901 cggggcgcgg ggcggtattg cgggggcgtg tgggggccga tggccagctg gcgggccatg
     6961 gcgtcattgc cccagtcgta atgcatggtg cgctcggcga tcagccagcc ggcgccgtgg
     7021 cgcgccagcc ggtccacata gcgcgccagc gagacgatct cgggcttggc ggcctgggta
     7081 tcgaagccca gcgtggtgac gcaataccgc gccaccgccg catcgccggt gatatccacc
     7141 gccagattgg ccaccagatg gcgcatcacg ctcagccccc cggcaggcgc cagaaagcgg
     7201 atctggttcg tgcgcgcggt tgcgccgatc tggtcatggc cggcggccag gatcgtgcca
     7261 ttgggcaggg tgatccgggc atctgcggca aacaccgaaa ggtaaaggtc ggcatcgagc
     7321 gtgtcgctgg cccaggcata gcgcaggacg acatcctcga tggccagccg gtcggcaaga
     7381 taggcaaggt tggtcatggt gtctcttcag gcaagggggg tggaaagggc gcgcggcgca
     7441 ggcccggcac tggcgcgccc gatcaccagc gccatgcccg ccaccatcgc cgcgccgccc
     7501 acaaacaggc cggcaaggcc ggtgtgcggc ttgatggcag cggtgaccat ggggctggca
     7561 aacacgccca ggaaggtgaa ggcattgaga tagcccagat accgccccgc cagcgccact
     7621 tcggtgcgct gggcgataat gtgatagaaa tagggccccg aaagccccat gcacaggcca
     7681 atggcgcccg cgccgatcgc cggcagcaca aggcccgtgc gcgccgtggc aaacagcagc
     7741 aggccggcca caaacagccc cagcgaggcg gtgaagctgc gggtggcgcc aaagcgatgc
     7801 tgcaccagcc cgaaaaacga cgccccggcg atcgaggtga ggcccggcag cccctggatc
     7861 agcgcgcgat cgcccgcccc atgggcgccg ttcagctcga gcaggaaggg cagctgcatg
     7921 ctggccatgc cgatcagccc gtaaaacagc agcaccagcc catagagcgg cagcaacgcc
     7981 gccagatatc cggttgaggc cccatcggcg cgggggcggc ggggcagcgg gcgctgctgg
     8041 tccatgccgg ccagcacgat gggcagcgcc agcgcggcaa agccggggta gatcagcagg
     8101 cacatccgcc agccgaaatg ctgcgtcagc gctccgccgg ccagcacgct gaggatggtg
     8161 gcgatgccgc cggtggcgcc tgcgccgccg aagaatttgg cgcgcccatc gcctgtatag
     8221 cgcgcgttga tccgccacag gcaggtggtg gcaatgcagg aggcggcaaa gcccaccacc
     8281 acgcgcccgg catagagcag cgccagatcc cccgtggcca cccccacgcc gccgcccagc
     8341 gcaaaggcca gcagcgcgcc cagaaaggcc cggcgcgtgc cggcccattc cagaatgcgc
     8401 ccgctggcca gcgcgcccat caccagcccc agcgcgctca tgcccagcat catctgggcg
     8461 gccagttcgc catggcgccc cagatcatgg gccatgtcgg gcaatgaggc ggtgatggcc
     8521 gcctcggcca ggccctgtgg aataaggccc agcatcatgg cca
//
LOCUS       DASRYE010000165         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_9580,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000165 DASRYE010000000
VERSION     DASRYE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9580"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1441)
                     /locus_tag="VFF98_08615"
     CDS             complement(41..1441)
                     /locus_tag="VFF98_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63733.1"
                     /translation="MALPALALGVAALLPAAGLDAAPAKPRAAPTAPWLEEPFPSTYA
                     PRARHDVLIAHATVLDGAGGRLDDGDVLLQNGRVVALGHGLKAPPGAETVDAHGRWVT
                     PGLIDVHSHDGTYVLPLTDIDEDSSDVSELETPNSANIRIENAINPQDAAFQRALENG
                     VTTLQVLPGSTPIFSGQAVVLKPLIAPSVAAMKFPAAPSGFKMACGENPKSFDAEQKR
                     GPTSRAGEIAYIRQAFLDAQRYREEWRAWAAGRTAEPPRRDLKMEALVNILEGRLRIN
                     MHCYRSDEMANMLAVAQEFGFHLTAFHHATEAYKIPGLLRAAGTCAAVWGDWWGFKME
                     ALDGIRANAPLLDKAGVCVTMHSDSPVSGQRLAIDAATAAAAGRAVGIALPPERLIRW
                     VTGNSAHMLGLEDRIGRLAPGYNADVVVWSGNPFSIYTKADLVLIDGALVFDRAHPPA
                     HAPSDFELGREGVPLP"
     gene            complement(1450..2067)
                     /locus_tag="VFF98_08620"
     CDS             complement(1450..2067)
                     /locus_tag="VFF98_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZU63734.1"
                     /translation="MNASPFGQYGPRDVADIVAAYPLAWAWAQGGDPGAGALLPMLGE
                     YDEAGRLTHLLGHMSRANPLIAALAGGDAVGFLFTGPQAYVSPEDAGRRDWGPTWIYA
                     RVAISGRITFLPAETRDAVEQLARHMEAGRPQPWTIAEIDHRAGPMLERIIAFRVAVD
                     AVHGWFKLGQDESPEVLRAILAATADPGMKGWIMRFNRDRLENGA"
     gene            2321..3775
                     /locus_tag="VFF98_08625"
     CDS             2321..3775
                     /locus_tag="VFF98_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZU63735.1"
                     /translation="MLRPLKLTLTDKIDPARKTPIYIQIIQAVVRDIERGRLPKGTYL
                     PSSRELAYALGVNRKTVVLAYEDLIAQGWLSAQGTRGTFVSNDLPDPSHRRAPPEDAP
                     ARADYRFTPPPARPLALPAGTIKLDEGAPDGRLFPAEPLARAHRAAIQRAARGNGFQY
                     RDPRGHPALREAVAAMLRSERGLSVSAANICITRGSQNGIFLAAQVLMQPGHAALFEE
                     LTYEPAVAAFRACGAQIVTAGLDENGIRVEDVEQACRRHAVRAIFLTPHHQFPTTVAL
                     RAERRLQLLNLAGQFGFAIVEDDYDHEFHFESQPLMPMAAYAPSHVIYVGSLSKLMLP
                     SLRIGYIAAPEKVINAIAHFVSVTDGMGNTITEDAAAELITSGEIKRHARKVRQTYLQ
                     RRDAYAEALRATLGERVEFALPDGGLAFWLRFRDPADLARIEAQGGAHGLSFASADSF
                     ALTPEAPRGLRLGFASLNDAERATALRTLKRILG"
     gene            3850..>4137
                     /locus_tag="VFF98_08630"
     CDS             3850..>4137
                     /locus_tag="VFF98_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZU63736.1"
                     /translation="MTTTTATTRRPAASLSGRAQPLARRMGRWHKWLGLALGLLWLVQ
                     AATGSLITFHWELTDLARRAPGAAHPFDAARLAARIEALEAGGWQLSSAWTS"
BASE COUNT          630 a         1438 c         1407 g          662 t
ORIGIN      
        1 cccgccagca gggcggccag catgagaagg gcagggcggc tcatggcagg ggcactccct
       61 ctcggcccag ttcgaaatcg gatggggcat gggcgggcgg atgggcgcga tcgaacacca
      121 gggcgccatc gatcagcacc agatcggcct tggtgtagat gctgaacgga ttgcccgacc
      181 acaccaccac atcggcgtta tagcccggcg ccagccggcc gatgcgatcc tccagcccca
      241 gcatgtgggc cgaattgccg gtgacccagc ggatcagccg ttccggcggc agggcaatgc
      301 cgaccgcccg gccggccgcc gctgccgttg ccgcatcgat cgccagccgc tggccgctga
      361 cgggggaatc ggaatgcatc gtcacgcaca cgcccgcctt gtcgagcagg ggcgcattgg
      421 cgcggatgcc atccagcgct tccatcttga agccccacca atcgccccac accgccgcgc
      481 aggtgcccgc cgcgcgcagc aggccgggga tcttgtaggc ctccgttgca tggtggaagg
      541 cggtgaggtg aaagccgaat tcctgcgcca cggccagcat gttggccatc tcgtcggagc
      601 ggtagcaatg catgttgatg cgcagccggc cttcgaggat attcaccagc gcttccattt
      661 tgaggtcgcg ccggggcggc tctgccgtgc ggcccgcagc ccaggcgcgc cattcttcgc
      721 gatagcgctg cgcatcgagg aaggcctggc ggatataggc gatttcgccg gcgcggctgg
      781 tggggccgcg cttctgttcg gcatcgaagc ttttggggtt ttcgccgcag gccatcttga
      841 agccggatgg cgctgccgga aacttcatgg ccgccacgct gggggcgatc agcggcttga
      901 gcaccaccgc ctggcctgaa aagatcggcg tggagccggg cagcacctgc agcgtcgtca
      961 cgccattttc cagcgcgcgc tggaaggcgg catcctgcgg gttgatggcg ttttcgatgc
     1021 ggatattggc cgaattgggc gtttccagct ccgacacgtc ggaggaatcc tcgtcgatat
     1081 cggtcagcgg cagcacatag gtgccatcgt ggctgtgcac atcgatgagg ccgggggtta
     1141 cccagcggcc atgggcgtcc accgtctcgg cgcccggcgg ggccttaagc ccatggccca
     1201 gcgccaccac acggccattt tgcagcagca catcgccatc atcgaggcgc ccgcccgcgc
     1261 catccagcac ggtggcatgg gcgatcagca catcgtggcg cgcgcgcggg gcataggtgc
     1321 tggggaaggg ctcttccagc cagggggcag tgggcgcggc gcggggtttg gcgggggcgg
     1381 catccagccc ggccgccggc agcagggccg ccacgcccag cgccagtgca ggcagggcca
     1441 tgcgccggct catgcgccgt tctccagcct gtcgcggttg aaacgcatga tccagccttt
     1501 catccccgga tcggcggtgg cggccagaat ggcgcgcagc acctcggggc tttcgtcctg
     1561 gcccagtttg aaccagccgt gcaccgcgtc aaccgccacg cgaaaggcga tgatgcgctc
     1621 cagcatgggg ccggcgcggt ggtcgatctc ggcgatggtc cagggctggg ggcggccggc
     1681 ctccatgtgg cgggccagct gctccaccgc gtcgcgggtt tccgccggca ggaaggtgat
     1741 gcgcccgctg atcgccacgc gggcatagat ccaggtgggg ccccagtcgc gccggccggc
     1801 atcctcgggc gagacatagg cctgcgggcc agtgaacagg aagccaaccg catcgccgcc
     1861 ggccagcgcc gcgatcagcg gattggcccg gctcatatgg cccagaagat gggtcagccg
     1921 gcccgcctcg tcatattcgc ccagcatcgg cagcagcgcg cccgcgcccg gatcgccgcc
     1981 ctgcgcccag gcccaggcca gcggataggc agcgacaata tcggccacat cacgcgggcc
     2041 atattggccg aaagggctgg cattcatata tgccatgcca tccggatggc gcagattatc
     2101 ctgcatgcgt ggttgttccc ttaccccggc cttatcccgg cacggctgtg cggccggccg
     2161 ccctgtgcct tgtgtcagcc tcagctatcc ggctggggct tggccccggc aagttccaag
     2221 gcaaataatt gcaaggggcc aacagggcgc tggcagcggg gcagggggca ggataccttg
     2281 gcccttgcga atcgcattat tcaacgaaaa tggcggtcca atgctgcgtc ctctcaagct
     2341 caccctcacc gacaagatcg atcctgcccg caagacgccg atctacatcc agatcatcca
     2401 ggcggtggtg cgcgatatcg agcgcggccg cctgcccaag ggcacgtatc tgcccagcag
     2461 ccgcgagctg gcctatgcgc tgggggtgaa ccgcaagacg gtggtgctgg cctatgagga
     2521 tctcatcgcc cagggctggc tgagcgcgca aggcacccgc ggcactttcg tctcgaacga
     2581 cctgcccgat cccagccacc gccgcgcgcc gccggaagat gccccggccc gggccgatta
     2641 tcgtttcacc ccgccgccgg cgcggccgct ggcgctgcct gccggcacca tcaagctgga
     2701 cgagggtgcc cctgatggcc gcctgtttcc cgcagagccg ctggcccgcg cccatcgcgc
     2761 cgcgatccag cgggcagcgc gcggcaatgg cttccagtat cgcgatccgc gcggccatcc
     2821 ggccctgcgc gaggcggtgg ccgcgatgct gcgctcggaa cgcggcctgt cggtctcggc
     2881 ggccaacatc tgcatcaccc gcggcagcca gaacggcatc ttcctggccg cccaggtgct
     2941 gatgcagccg ggccatgccg ccctgttcga ggaactgacc tacgagccgg cggttgccgc
     3001 cttccgcgcc tgcggggcgc agatcgttac tgccgggctg gatgagaacg gcatccgcgt
     3061 ggaagatgtg gagcaggcct gccgccgcca tgcggtgcgc gccatcttcc tcacgccgca
     3121 ccaccagttt cccaccacgg tggccctgcg cgccgagcgg cggctgcaat tgctgaacct
     3181 ggccggccag ttcggctttg ccatcgtgga ggatgactac gaccacgagt tccacttcga
     3241 gagccagccg ctgatgccga tggccgccta tgcgccctcg catgtgatct atgtcggctc
     3301 gctgtcgaag ctgatgctgc catcgttgcg catcggctat atcgccgcgc cggaaaaggt
     3361 gatcaatgcg atcgcccatt tcgtctcggt caccgatggc atgggcaaca ccatcaccga
     3421 ggatgccgct gccgagctga tcaccagcgg cgagatcaag cgccacgccc gcaaggtgcg
     3481 ccagacctat ctgcagcgcc gcgatgccta tgccgaggcg ctgcgggcca cgctgggcga
     3541 gcgggtggaa tttgccctgc ccgatggcgg gctggccttc tggctacgct ttcgtgatcc
     3601 ggccgatctg gcgcggatag aggcgcaagg gggcgcccat gggctcagct ttgccagcgc
     3661 tgattccttt gccctcacgc cagaggcgcc gcgcgggttg cggctgggtt ttgccagcct
     3721 caacgatgcc gaaagggcca ccgcgctgcg cacgctgaag cgcattctgg gctgagcggt
     3781 gcccgtggcc cgccccgccc ggcgttcttg gcggttggcg ggcggcaggc ggggcccgat
     3841 aggggccgca tgacgacgac caccgccacc acccgccgcc ccgccgcttc cctttccggc
     3901 cgcgcgcagc cgctggcccg gcgcatgggg cgctggcaca aatggctggg gctggcgctg
     3961 ggcctgctgt ggctggtgca ggcggccacc ggcagcctca tcaccttcca ttgggagctg
     4021 accgatctgg cccgccgcgc gcccggcgcg gcccacccct tcgatgccgc tcgccttgcc
     4081 gcgcgcatcg aggcgctgga ggccggcggc tggcagctgt cttccgcctg gacgagc
//
LOCUS       DASRYE010000166         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_133283,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000166 DASRYE010000000
VERSION     DASRYE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133283"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1056
                     /locus_tag="VFF98_08635"
     CDS             142..1056
                     /locus_tag="VFF98_08635"
                     /inference="COORDINATES: protein motif:HMM:NF027545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HZU63737.1"
                     /translation="MPLPSASPRERIAREAAGWLAKWQAGTLDEAAFEAWRGADPAHA
                     VAFARALAIWQQAGAAPAPAPETAPAPAPARRVAGLAALVGAGLLLAAGGFGARAFAW
                     QSAQSVVGQSLSVALAPAAHAQLNTNSRISWRFSGAERHVWLEAGEMALDIATPTLLD
                     CGGEQVWLASGQYNARIRAGACNILVLGHGGSARTAEGIAAGPQQNLLIAGGHPAARA
                     ASALEVAQVEAWPKGEILFEGLALSAAVDEYNRYLTRKITIVDPDLAGIAVGGRFTTT
                     DPAAFLSALQLGIGVKVVPSANGYLVTR"
     gene            1180..3618
                     /locus_tag="VFF98_08640"
     CDS             1180..3618
                     /locus_tag="VFF98_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63738.1"
                     /translation="MFSRSFAAPLVAALMVSAAGALPAAAAAHAAAPQADFHIPAQPL
                     AGALTSFARQADVQIFFPAASIKGLMAPAIDGRMSRQAALRRLIAGSRLTVRQDDGKT
                     VVLGEADNAEDETPVAAAQSITVIGHAQTLAPSSMPGDVTQPTSVIQENFIANNIVPL
                     ASIDDVIKFQPSVWTQNPNGPGIGKAEEMVIRGFADGQYNMTFDGIPFGDSSDLHHTT
                     SSLFIAHALKEAQIDRGPGTASTIGKATFGGSVGFLSKDPLKTFDIDAYGTAGSFNTL
                     AGGGRIDSGQMGPVRLFAEAQHEQTDGYLTHSNENRDNFMGKAIIDVSAKTKITAMAN
                     WNREFQYTTQGATLAEYAKYGNNYGLCGNAAAMCYYGYQPSHYYSDFEYVRLNTSIGN
                     VLVDNNLYHNGFVHSYIESSDASDDNTADNGVTFYSATTIGKKVATYSSDIKGKLAHA
                     KVSTWGDTLRVTVPGKIVDIKTGLWLDFQHDDRYSETVDWSQNLIPVTGKYGTAYSYQ
                     FSDLGTTVQPYLELVVRPIDGLVLNPGVRYTSYNRTMYATVNKGTNAAAADNVTYHAV
                     QPSIAANYTILPGWTAYAQAARGFLAPPVAVFQVNQVGSLKPETTWNYQLGTVAHGHH
                     WSVSFDGYYINFSNYLSSLTTGGQTYYGNTSGAIYKGLELEGQVVVAKGLSLYGNATL
                     NSAKYKGVNVTLEEAPEWTAAAGILYEDKHGPYASLIGKAVGPRWGNDTTGTYDDPSS
                     YVGTTFTADLAAGWHFSRFFGMLRNVTPSVKIGNLFNSHAISDYAGTQSATNAALFWR
                     TPGRSFFFNLEAHL"
BASE COUNT          657 a         1329 c         1152 g          609 t
ORIGIN      
        1 agaactgccg gcgccagcgc ccgcctctcc ccccgcgccc gcggccatcg cccgatagac
       61 aagccacccc agctgggcta gcgcgggcct ttgcgccgcc cttcccctta tcgcacccca
      121 cctcccgcag caggttccgc catgcccctc ccttccgcct ccccccgcga gcgcatcgcg
      181 cgtgaggcgg ccggctggct ggcgaaatgg caggccggca cgctggacga ggccgctttc
      241 gaggcctggc gcggggcaga tcccgcccat gccgtggcct ttgcccgggc gctggcgatc
      301 tggcagcagg ccggcgccgc gcctgcccca gcgccagaaa ccgcgcccgc gcccgccccg
      361 gcccggcgcg tggccggcct tgccgcgctg gtgggggcag ggctgctgct ggccgccggc
      421 ggctttggcg cgcgggcctt tgcctggcaa agcgcgcaat ccgtggtggg gcaaagcctg
      481 tctgtggcgc tggcgccggc cgcccatgcc cagctcaaca ccaacagccg catcagctgg
      541 cgcttttccg gggcagagcg gcatgtctgg ctggaagcgg gcgaaatggc gctggatatc
      601 gccacgccca ccctgctgga ttgcgggggc gagcaggtgt ggctggccag cggccagtat
      661 aacgcgcgca tccgggcggg cgcctgcaac atcctggtgc tgggccatgg gggatctgcc
      721 cgcacggccg aagggattgc cgcgggcccc cagcagaacc tgctgatcgc cggcggccac
      781 ccggccgcac gcgcggcctc ggcgctggaa gtggcgcagg tggaagcctg gcccaagggc
      841 gagatcctgt tcgaggggct ggcgctgagc gcggcggtgg acgaatacaa ccgctatctc
      901 accaggaaga tcaccatcgt cgatccggat ctggccggca ttgccgtggg cgggcgcttc
      961 accaccaccg atccggccgc tttcctctcg gcgctgcaac tgggcatcgg ggtgaaggtg
     1021 gtgccctcgg ccaatggcta tctcgtcacc cgctgaacgg gcgggcggca caagcgccac
     1081 ctgaaaaaaa tctgcgcccg gcttggcggt tttgccgcaa ggcttcgtct tcccccgcga
     1141 ggcgccggca atggcgcatt gttcccaagg ggtattgcca tgttttcccg ttcgtttgcc
     1201 gcgccgctgg tggccgcatt gatggtttcc gccgcgggcg ccctgccggc tgccgctgct
     1261 gcccatgccg ccgccccgca ggccgatttc cacattcccg cccagccgct ggccggcgcg
     1321 ctcaccagct ttgcccgcca ggccgatgtg cagatcttct ttcccgccgc cagcatcaag
     1381 ggcctgatgg ccccggcgat cgacgggcgg atgagccgtc aggccgccct gcgccgcctc
     1441 atcgccggca gccggctgac cgtgcgccag gacgacggca agaccgtggt gctgggcgag
     1501 gccgacaacg ccgaggatga aacacccgtt gccgccgccc agtccatcac cgtgatcggc
     1561 catgcgcaaa cgctggcccc ttcctcgatg cccggcgatg tgacccagcc gacctcggtg
     1621 atccaggaaa acttcatcgc caacaacatc gtgccgctgg cctcgatcga cgatgtgatc
     1681 aagttccagc ccagcgtgtg gacacagaac cccaacggcc cgggcatcgg caaggccgag
     1741 gaaatggtca tccgcggctt tgccgatggc cagtataaca tgaccttcga cggcatcccg
     1801 tttggcgaca gttcggacct gcaccacacc acttcctcgc tgttcatcgc ccatgcgctg
     1861 aaggaagccc agatcgaccg cggccccggc accgccagca ccatcggcaa ggccactttc
     1921 ggcggctcgg tgggcttcct ctcgaaggat ccgctcaaga ccttcgacat cgacgcctat
     1981 ggcacggccg gcagcttcaa cacgctggcc ggcggcgggc gcatcgacag cgggcagatg
     2041 ggccctgtgc gcctgttcgc cgaagcccag cacgaacaga ccgacggtta tctcacccat
     2101 tccaacgaga accgcgacaa tttcatgggc aaggcgatca tcgacgtttc cgccaagacg
     2161 aagatcaccg ccatggccaa ctggaaccgc gagttccaat ataccacgca gggcgcaacg
     2221 ctggccgaat atgccaaata tggcaataat tatggcctgt gcggcaatgc cgccgccatg
     2281 tgctattatg gctatcagcc cagccattat tattcggatt tcgaatatgt ccggctgaac
     2341 accagcatcg gcaacgtgct ggtggacaac aacctctacc acaacggctt cgtccacagc
     2401 tatatcgaaa gctccgacgc ttccgacgac aacaccgccg acaatggcgt gaccttctac
     2461 agcgccacca ccatcggcaa gaaggtggcc acctattcca gcgacatcaa gggcaagctg
     2521 gcccacgcca aggtgagcac ctggggcgac acgctgcgcg tgaccgtgcc gggcaagatc
     2581 gtcgacatca agaccggcct gtggctcgat ttccagcacg acgaccgcta ttccgaaacg
     2641 gtggactgga gccagaacct gatccccgtc accggcaagt atggcacggc ctattcctat
     2701 cagttcagcg atctgggcac caccgtgcag ccctatctgg aactggtggt gcgcccgatc
     2761 gatggcctcg tgctgaaccc gggcgtgcgc tacaccagct ataaccgcac catgtatgca
     2821 acggtgaaca agggcaccaa tgccgccgcg gccgacaatg tgacctacca cgccgtgcaa
     2881 ccctcgatcg cggccaacta caccatcctg cccggctgga ccgcctatgc ccaggccgcg
     2941 cgcggcttcc tcgcgccgcc ggtggcggtg ttccaggtca atcaggtcgg ctcgctcaag
     3001 cccgagacga cgtggaacta tcagctgggc acggttgccc atggccacca ctggtcggtc
     3061 tcgttcgatg gctattacat caatttctcg aactacctct cctcgctgac gaccggcggc
     3121 cagacctatt atggcaacac ctcgggcgcc atctacaagg ggctggaact ggaaggccag
     3181 gtggtggtgg ccaagggcct gtcgctttat ggcaatgcca cgctcaattc ggccaaatac
     3241 aagggcgtga acgtaacgct ggaggaagcg ccggaatgga ccgcggcggc cggcatcctc
     3301 tatgaggaca agcacggccc ctatgcctcg ctgatcggca aggcggtggg cccgcgctgg
     3361 ggcaacgaca ccaccggcac ctacgacgat ccgtccagct atgtcggcac caccttcacc
     3421 gcagacctgg ccgccggctg gcatttcagc cgcttcttcg gcatgctgcg caatgtcacc
     3481 ccgtcggtga agatcggcaa cctgttcaac agccacgcca tctccgatta tgccggcacc
     3541 cagtcggcca ccaatgcggc gctgttctgg cgcacgccgg gccgcagctt cttcttcaac
     3601 ctggaagcgc atctgtgagg cagggcttgt tccgcaagcc cagcgccatg gtgctggcca
     3661 tggtgctggg cggcgtgagc gcgggcgtgg gggccaaaac ccccgcgccc gttcctgcct
     3721 ccgccggcac atggcagatg gaccggg
//
LOCUS       DASRYE010000167        13447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_151043,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000167 DASRYE010000000
VERSION     DASRYE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151043"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFF98_08645"
     CDS             <1..257
                     /locus_tag="VFF98_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63739.1"
                     /translation="RWVPASATRKGEITFWLDGQQVGPRVSYDPYVEVPPPAQPDAIQ
                     PFAWLDRHHLVLILGGAPDNPISVRHVVVWQKDASQNLRN"
     gene            complement(269..1768)
                     /locus_tag="VFF98_08650"
     CDS             complement(269..1768)
                     /locus_tag="VFF98_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HZU63740.1"
                     /translation="MAFDPVARALVGALKQNLSSTVSGSDGDSYVGSNDGAAGSMWTS
                     VAGFINYLRGNSGSGVLGFLPAGTGAVARTVQAKLAENVSVKDFGAKGDGSTDDTAAI
                     QAAITAVGAAGGGRLLVPPGTYKISAQITAAANVNLYANYSARNTPATMQAASGFTGR
                     MIYVPQAAASFSVSGISFLSTVTADSSIYIDASSVGVSYCGFNFSGRAGVELSANSIA
                     CEVAHCTGTNLVYQRSALTAAVGGLHVLGTDHYITDNEFNCWSGNNTVVSSGAYACSI
                     YIGGANHFLVGNIGEFGETGVWVDANKSRFTGNRADRCMGHGWVIPGASNCFTACQAV
                     QCGTGATNSYDGWQISGVLNRFVGCMVPYLGQSTLRYGWNDKVDSYTQKNEMVGCSCD
                     EAYGTSAYHNNFDGGGFLFGTGFYTQTSGSVIDVSGKTNIVLYYTTAQTVTQMTGGVN
                     GQTVRLMAVNGNVTVHNNQFKTLTGADIVLQQFAVYEFTWWNYGWMQHA"
     gene            complement(1781..2392)
                     /locus_tag="VFF98_08655"
     CDS             complement(1781..2392)
                     /locus_tag="VFF98_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63741.1"
                     /translation="MSGPSILHPAPYVPSQALAFADVDGTALLVSKSLALPVCVQNSA
                     PITVSGNVAIANQAGGVIAISAASLPLPASAATAANQAAILSALQGATPAGGNLIGKV
                     STTFTASGLSPVTGSVSAGAQSGSFGPMPGRSFNLTLVPTGSGTNAAQVERQFTGDAT
                     WYQAVAPATVASLPNSFAWQEDEAGVTYRVNVLSGAFVVRISQ"
     gene            2575..2991
                     /locus_tag="VFF98_08660"
     CDS             2575..2991
                     /locus_tag="VFF98_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU63742.1"
                     /translation="MPHVSQPAQSLTAVHRFTRGLRLPGLALALAAAGFAAPALAGEP
                     PAVFARCAVCHNAAPGAPDKVGPNLWGVYGRKAASTKYNYSAALKGAKLRWDEATLDK
                     WISGPMTMVPGTMMSFPGIKDAAKRAEIIAFLKQAH"
     gene            complement(3090..5084)
                     /locus_tag="VFF98_08665"
     CDS             complement(3090..5084)
                     /locus_tag="VFF98_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63743.1"
                     /translation="MAPGSALAADADMGAEAPGGAIVVTGHGLADTPAVPAYDVQTLG
                     REALLNAPSGRIEDVLGNVAGFQEYRRSDSRAANPSAQGVTLRALGGNAASRTLVLLD
                     GVPMADPFFGSVALSALAPERLSSARVTRGGGSGAFGAGAVSGTIELNSANAQEMGLL
                     NAEAAVDNRAESAVSATLSPQLGNGFVQVSGRWDRGEGFWTTPVAQRVPASVRAAYEG
                     WSGQVRLVQQVGASSELQARLMSFGDNRTLRFAGANSMDRGSDASIRLVGHGRWAYDV
                     LAYGQLRDFANVVISSTTYKPTLNQFKTPTTGYGGKAELRPPVGHGQVLRLGADVRVA
                     QGNEQERALNASTGALTATRKEGGRNSDVGLYLEDDWTLGRLVLTGGARADRWDIDNG
                     AFTQLSPAGVVTTTTTYPDRGGWAGSFRGGARFQLTPDVAVRAAGYSSLRSPTLNELY
                     RSYNIGSVTNKANAALTNERLVGYEAGVEMALGKVGRLEVTAFDNQLRHAIANVTIAA
                     NTQQRQNVDAIRARGVEATLGLKAGAFSLDGSLAYTEARVESHGSQKALNGLAPPQTP
                     SIAISTTLGWRPRAGWLAQLGLRHVGDQYEDDLHTGLLPRATTINALVQAPLGHGFSF
                     SVRGENLADVSVITRNQAGSLDVGAPRTIWFGLRYGGHGG"
     gene            complement(5231..6649)
                     /locus_tag="VFF98_08670"
     CDS             complement(5231..6649)
                     /locus_tag="VFF98_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZU63744.1"
                     /translation="MRKTKLLGGAAIALALCLGATSAEASTASDLLKRLHDKGILTDD
                     EYAALQAEEKADQAKAAEAVAAAAPGGQPAAPAQIDDRRFVKMTDSGVGLVVGPATIK
                     FSGSVNGFYVHDSPQKAGANTAVTGGLATVGSGNSSAVRNGLLPGFFKIDVTTTQGGW
                     NVGAHFGMYPGINSNSWSGLSANSAGQPTAFATSGIDFRQTYLTFGRAGFGEVKIGRD
                     IGLFGSDAILNDITLLTPGSPGSNTAPGNTTLGRIGVGYIYTDFQPQITYTSPAYKGF
                     QAAVGIFQPLDSLSGSAQNNSEPGFQAKLTYDGKIQGAGLHLWMSGLTQKHDVISGGS
                     YTGRGLDAGAKVTVGPVAVTGYYYTAKGLGTEALNLFDTDVFGNTRGSEGFYVQAMAT
                     FGKWSAGGSYGASYLHYANAADALANPTLVSSNASGVGQIRYGLNSWVTLISEYTHTT
                     AKAHNGNKDDSDTMAFGGILFF"
     gene            complement(6803..7753)
                     /locus_tag="VFF98_08675"
     CDS             complement(6803..7753)
                     /locus_tag="VFF98_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HZU63745.1"
                     /translation="MWSVLAAGLAGPALAGPTAYVSNERGNSVTVIDIDARQVLANWP
                     VGQRPRGIALSRDGARLFIAAGTDNTVEVRDRITGKLLAHLPSGQDPEQFFPSRDGKS
                     LFVANEDDAATTAIDLASGQVAWQVPVGREPEGITQSPDGKWLVVTSEDQHNIAWIDL
                     AAHTNTGITATEHRPRHVEFTPDGHELWVAAEAGQVVQVVDPASRKIVATIPFAPPGV
                     SAYKVLPCGIRFTHDGTKAWVALGRANHIALVDVATRKVLAYVPVGGRPWHLALSPDE
                     RQVLVANGMSDDVSVVDVASLKVVASVPAGSGPWGVAIAP"
     gene            7964..8407
                     /gene="pedF"
                     /locus_tag="VFF98_08680"
     CDS             7964..8407
                     /gene="pedF"
                     /locus_tag="VFF98_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HZU63746.1"
                     /translation="MSGKLGRAVLIGAMLLATASMGSRIWAHGNVVPQPVDTSALPDI
                     EGGNDNWVQHNPYRGNAKAIAIGESAYGQNCARCHGLDAISGGIAPDLRYLELGDSGD
                     EWFVQRYHHGSSHDGKVYMPPMGEILGQKAGWAIRAWLETKHTDN"
     gene            8409..9260
                     /locus_tag="VFF98_08685"
     CDS             8409..9260
                     /locus_tag="VFF98_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63747.1"
                     /translation="MIARRALLGALAASAGSLLAPCALQAAPLAKVKEQGRLRIAVYQ
                     DNRPWSWADGAGLHGIDVDLGKALAAQLGVAAEVVDFMPGDDLAADLRNTVWRGGLLG
                     FQPCDVMLHVPFDHELQLANDQVAILAPYCREGFAMLCGSDVDCEAPPPHFRGLRLAA
                     ETASASDAYLLGAFGGVLRGDVHHFRNGYDAAAAVGSGQADMAMATAAQVEAASHDAT
                     ASLHRRRGPLPAMSSPGWDVAMAVKENSRTLGDALENIIGKMVANGQMAALFAAHGVS
                     WKAALAG"
     gene            9538..11286
                     /locus_tag="VFF98_08690"
     CDS             9538..11286
                     /locus_tag="VFF98_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HZU63748.1"
                     /translation="MKGSFKRSMAGFATLALAAAMPAWAENGPTSQDILNDANTPGDV
                     LSYGMGPKAQRFSPLNAVNTSTVKNLAPAFAASLGGEKQRGQEAQPLVYDGTIYVTAS
                     YSRVFAFDSHTGEKKWEYDARLPDGIMPCCDVVNRGAAIHGDKIIFATLDAHLIALNR
                     HTGKVIWNQKMADYSAGYSATAAPLIVKDMVIYGNSGGEFGIVGEVQARDVNTGALIW
                     SRPTVEGNIGTLHGQPNGMTGKLNATWQGDQWKTGGGATWLGGTYDPETNLIYMGTGN
                     PGPWNSHDRPGDNLYTASTLAIDADTGVIKWHYQTTPHDGWDFDGVNEFIPFDATING
                     KAMKLGAKADRNGYFYVLDRTNGKFISASKFVMQTSWANGINAKTGRPNFIDENRPGA
                     PNGADKGKVVFASPSFLGGKNWMPMAYSQDTGLFYIPSNDWGMDIWNEPIAYKKGAAY
                     LGAGFTIKPIAEDHIGALRAMDPKTGKIVWEYKNKAPLWGGVLTTGGGLVFTGTPEGY
                     LKAFDAKTGEELWKFQTGSGVVGSPITWEQDGEQYVAVMSGWGGAVPLWGGEVAKTFK
                     EISQGGMLWVFKLPKH"
     gene            11393..12037
                     /locus_tag="VFF98_08695"
     CDS             11393..12037
                     /locus_tag="VFF98_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU63749.1"
                     /translation="MTTTMERVLIVDDHSLVRDGMRSLLEISFPHCEILEAGALAEAV
                     ATLEDQGDVDMVLLDLNIPDVTRFSGLSALRETFPSIPVVMVSGTLDRITVREALAAG
                     AAGFVPKSLKRGEIVEALRQVLEGEIYIPDMFDEDDETSREHAAILTRIETLTPQQKV
                     VLRHLVNGKLNKQIAYELDVSMTTVKAHVSAILQKMNAFSRTQAVILANRVGFH"
     gene            complement(12097..>13447)
                     /locus_tag="VFF98_08700"
     CDS             complement(12097..>13447)
                     /locus_tag="VFF98_08700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZU63750.1"
                     /translation="MAPGQHRAGRCQLGERVVEIRSTPIQPDGMAFSFDDVTERIAYE
                     ERLRDVAETLERRVAERTTALEAEVAERRAMEAELLAAKTAAELASRSKTSFLAAASH
                     DLLQPLNAARLFVSALAERRLAVPSRALVRQAGVALDSVEELLEALFEISRLDAGAVQ
                     PEIAAIELDRMLGALRIEFMAQAKQAGLALDIPDTGLWVKSDFRMLRRILQNFLSNAL
                     RYTGQGSVAVRVESDGAIVRIAVKDTGRGIDPAHFDVIFEEFRRLSAQRGPSGKGLGL
                     AIVKRASQMLGHRLEVASQPGEGSEFAVLVPLSARLAEEAPLPRPRERGLAAATGTVL
                     IIDNEPAILEGMQALLGKWGYGVATAGTLEEARAQVLGEAPPALIIADYHLDDDQLGD
                     EMVAALRAECGVEIPALVISADRTAEVKARLTAAGLPLLNKPVKPAQLRALLRMMMA"
BASE COUNT         2373 a         4761 c         4133 g         2180 t
ORIGIN      
        1 cccgctgggt gcccgccagc gccacccgca agggcgagat caccttctgg ctcgacgggc
       61 agcaggtcgg cccgcgcgtc agctacgatc cctatgtcga ggtgcccccg cccgcccagc
      121 ccgatgctat ccagcccttc gcctggctcg atcgccacca cctggtgctg atcctgggcg
      181 gcgcgccaga caaccccatc tccgtgcgcc atgtcgtcgt ctggcagaaa gacgccagcc
      241 agaacctcag gaactaggcc cgccccgctc aggcgtgctg catccagcca taattccacc
      301 aggtgaattc atagacggca aattgctgca agacgatatc cgccccggtc agcgtcttga
      361 actggttgtt atgcaccgtc acattgccgt tcaccgccat cagccgcacc gtctgcccgt
      421 tcaccccgcc ggtcatctgg gtcacggtct gggcggtggt gtaatagagc acgatattgg
      481 tcttgccgct cacatcgatc acgctgccgc tggtctgggt gtaaaagccg gtgccgaaca
      541 gaaagccgcc gccatcgaaa ttgttgtgat aggccgatgt cccataggcc tcgtcgcatg
      601 aacagcccac catctcgttc ttctgcgtat agctgtccac cttgtcgttc cagccatagc
      661 gcagcgtcga ctggcccaga tagggcacca tgcaccccac gaaacggttg agcaccccgc
      721 tgatctgcca gccatcatag ctgttggtgg cgccggtgcc gcattgcacc gcctggcagg
      781 cggtaaagca gttggatgcc cccgggatca cccagccatg ccccatgcag cgatcggccc
      841 ggttgccggt aaagcgcgac ttgttggcat ccacccacac gcccgtctcg ccaaactcgc
      901 caatattgcc gacgagaaag tggttggccc caccaatata gatcgaacag gcataagccc
      961 ctgaagacac caccgtatta ttgcccgacc agcagttgaa ctcattgtcg gtgatgtaat
     1021 gatcggtgcc cagcacatgc agcccgccca ccgccgccgt cagggcgctg cgctgataga
     1081 caaggttggt ccccgtgcaa tgggccacct cgcaggcaat cgaattggcc gaaagctcca
     1141 cccctgcgcg cccggaaaaa ttgaagccgc aatacgacac ccccaccgag gaggcatcaa
     1201 tatagatcga gctgtcggcc gtgaccgtcg agagaaagct gatccccgaa accgagaacg
     1261 aggccgccgc ctgcggcaca tagatcatcc gcccggtaaa accggatgcc gcctgcatgg
     1321 tcgccggcgt gttgcgggcg gaataattgg cataaaggtt cacgttggcc gccgccgtga
     1381 tctgcgcgga aatcttgtag gtgcccggcg gcaccagcag gcgcccgccg cctgcggccc
     1441 ccaccgcggt gatcgcggcc tggatggcgg cagtatcgtc ggtgctgcca tcgcccttgg
     1501 cgccaaaatc cttcaccgac acgttttcgg ccagcttggc ctgcaccgtg cgcgccaccg
     1561 cccccgtgcc ggcgggcaga aagcccagca cgcccgagcc cgaattgccg cgcagatagt
     1621 tgatgaagcc ggccacgctc gtccacatgg agccggccgc cccgtcgttc gagccgacat
     1681 agctgtcgcc atccgaaccc gaaaccgtgg aggacagatt ctgcttgagc gcccccacca
     1741 gcgcccgtgc aacaggatcg aaagccattt cccgcccccc ttactggctg atgcgcacca
     1801 caaaggcgcc cgacagcaca ttcacccgat aggttacccc cgcttcatcc tcctgccagg
     1861 caaagctgtt gggcaggctg gccacggtgg ccggcgccac tgcctgatac caagtggcat
     1921 cgccggtaaa ctggcgctcc acctgggcgg cattggtgcc gctgccggtg ggcaccaggg
     1981 tcaggttgaa actgcgcccc ggcatcggcc caaaactgcc agactgcgcc ccggcgctga
     2041 cactgccagt caccgggctc agccccgagg cggtgaaggt ggtcgacacc ttgccgatca
     2101 gattgccgcc ggccggcgtt gccccctgca gcgcggaaag gatcgcggcc tggttggcgg
     2161 ccgtcgccgc gcttgccggc agtggcaggc tggccgcgct gatggcaatc accccccccg
     2221 cctgattggc aatcgccaca ttcccgctca ccgtgatcgg cgcgctgttc tgcacgcaca
     2281 ccggcagcgc caggctcttg ctcaccagca gcgccgtgcc atccacatcg gcaaaggcaa
     2341 gcgcctggct gggcacatag ggtgcaggat gaagaatcga tggcccggac atatcggctc
     2401 cttaacgaat cagatgaacc gttaattaac caaaccggtt caggtaggaa aacaaaaaga
     2461 gaacatatca ccagccatcg ccgccaccag ccggcgtcca cctcaaccaa agtgcaattt
     2521 cgccgcgccg ccagccatgg cacggcaggc acaaatcata ctgggagatc ctgcatgcct
     2581 cacgtttcac agccggccca atcgctcacc gcagtgcacc gcttcacgcg cggcctgcgc
     2641 ctgcccgggc tggcactggc cctggcggcc gcgggctttg ccgcccctgc cctcgcgggt
     2701 gaaccgccgg ccgtttttgc ccgctgcgcg gtgtgccaca acgccgcccc cggcgcgccc
     2761 gacaaggtcg gccccaacct gtggggcgtc tacggccgca aggcagcctc cacgaaatac
     2821 aattattcgg cagccctcaa gggcgccaag ctgcgctggg acgaggccac gctcgacaag
     2881 tggatctccg gcccgatgac gatggtgccc ggcacgatga tgtccttccc cggcatcaag
     2941 gatgcggcca agcgcgccga aatcatcgcc ttcctgaagc aggcgcactg atctgaagcc
     3001 ggcccgctaa ccgcttcagg acacagccgc acaagcggca ccagaaggcg gggttccctc
     3061 ccggaacccc gcctttccca tgcccctatt cagccaccat gcccgccata gcgcaggcca
     3121 aaccagatcg tgcgcggcgc gcccacatcc agcgaaccag cctggttgcg ggtaatcacg
     3181 ctcacatcgg ccaggttctc cccccgcacg ctaaagctga aaccatggcc cagcggcgcc
     3241 tgcaccagcg cgttgatggt cgtcgcgcgc ggcagcaggc cggtatgcag atcgtcctca
     3301 tactggtcgc ccacatggcg caggcccagc tgcgccagcc atcccgcccg cggccgccag
     3361 cccagcgtgg tgctgatggc aatgctgggc gtttgcggcg gagccagccc attcagcgcc
     3421 ttctggctgc catggctttc cacccgcgcc tcggtatagg ccagcgatcc atccagcgag
     3481 aacgcccctg ccttcagccc cagcgtcgcc tccacgccgc gcgcgcggat ggcatccaca
     3541 ttctggcgct gctgggtgtt ggccgcgatc gtcacattgg caatggcatg gcgcagctgg
     3601 ttgtcgaaag cggtcacttc cagccgcccc accttgccca gcgccatttc cacgcccgcc
     3661 tcatagccca ccagccgctc gttggtaagc gccgcattgg ccttgttggt caccgagccg
     3721 atgttgtagc tgcgataaag ctcgttcagc gtcgggctgc gcaggctgga atagccggcc
     3781 gcgcgcaccg caacatccgg cgtcagctgg aagcgcgcgc cgccccggaa actgcccgcc
     3841 cagccgccac gatcgggata ggtggtggtc gtcgtcacca cgccggccgg gctcagctgg
     3901 gtgaaggcgc cattgtcgat atcccagcgg tcggcccgcg cgccgccggt cagcaccagc
     3961 cggcccagcg tccagtcatc ttccagatag aggcccacat cgctgttgcg cccgccttcc
     4021 ttgcgggtgg ccgtcagcgc gccggtgctg gcattcagcg cccgctcctg ctcgttgccc
     4081 tgggccacgc gcacatccgc ccccagccgc agcacctgcc catggcccac cggcggccgc
     4141 agctcggcct tgccgccata gccggtggtc ggcgtcttga actggttcag cgttggcttg
     4201 taggtggtgg aagaaatcac cacattggca aaatcccgca attgcccata ggccagcaca
     4261 tcataggccc agcgcccgtg gcccaccagg cggatgctgg catcgctgcc cctgtccatg
     4321 ctgttggcgc cggcaaagcg cagcgtgcgg ttgtcgccaa agctcatcag ccgggcctgc
     4381 aattcgctgc tggcgcccac ctgctgcacc agccgcacct ggcccgacca gccctcatag
     4441 gccgcccgca cgctggccgg cacgcgctgc gccacaggcg tcgtccagaa gccctcgccc
     4501 ctgtcccagc ggccggaaac ctgcacaaag ccattgccca actgcggcga cagcgtggcc
     4561 gaaacggcgc tttcggcgcg attgtccacc gccgcctcgg cattcagcag gcccatttcc
     4621 tgggcattgg cgctgttcag ctcaatcgtg ccggaaaccg cgcccgcgcc aaacgccccg
     4681 ctgcccccgc cccgcgtcac ccgcgccgag gacagccgct cgggcgccag cgccgacagc
     4741 gccaccgagc caaagaacgg atcggccatc ggcacgccat ccagcagcac cagcgtgcgg
     4801 ctggcggcat tgccgcccag cgcgcgcagc gttacccctt gcgccgaagg attggccgcc
     4861 cggctgtccg agcggcgata ttcctgaaag cccgcgacat tgcccagcac atcctcgata
     4921 cggccggaag gggcattgag cagcgcctcg cgccccagcg tctgcacgtc ataggcgggc
     4981 actgcgggcg tatcggccag cccgtggccg gtcaccacaa tggcgccgcc gggcgcctct
     5041 gcgcccatat ccgcgtcggc cgccagcgcg ctcccgggcg ccatcaacgc ggccagcccc
     5101 gcccccaaat gccaagcgcg cattttgccc ttcatgtcct ctccccgaaa caattggccg
     5161 cgaccgctgc ccaaccagcg atcgcggcct tttttatcta acccgtttca acaatagacg
     5221 aatatcagcc tcagaagaag aggataccac caaaggccat ggtatcgctg tcatccttat
     5281 tgccattatg cgccttggcg gtggtgtggg tatattcgct gatcagcgtc acccagctgt
     5341 tgaggccata gcggatctgg cccacgccgc tggcattgct gctgacaagc gtggggttgg
     5401 ccagcgcatc ggcggcattg gcatagtgca gatagctggc gccatagctg ccgcccgcgc
     5461 tccacttgcc gaaggtggcc atggcctgca catagaagcc ctcgctgccc cgggtgttgc
     5521 cgaacacgtc ggtgtcaaac aggttcagcg cttcggtgcc caggcccttg gcggtgtagt
     5581 aatagccggt caccgccacc gggcccaccg tcaccttggc gccggcatcg aggccgcggc
     5641 cggtgtagga accgcccgag atcacatcat gcttctgcgt caggcccgac atccacaggt
     5701 gcaggccggc gccctggatc ttgccatcat aggtcagctt ggcctggaag ccgggctcgc
     5761 tgttgttctg ggccgaaccg ctcagcgaat cgagcggctg gaagatgccc accgcagcct
     5821 ggaagccctt gtaggccggc gaggtatagg tgatctgcgg ctggaaatcg gtgtagatat
     5881 agcccacgcc gatgcggccc agcgtggtgt tgccgggcgc ggtgttgctg ccgggcgagc
     5941 cgggggtgag cagggtgatg tcgttgagga tggcatcgga gccgaacagg ccgatatcgc
     6001 ggccgatctt cacctcgcca aagccggccc ggccgaaggt caggtaggtc tggcgaaagt
     6061 cgatgccgct ggtggcaaag gcggtgggct ggccggcgct gttggcgctc agcccgctcc
     6121 acgaattgct gttgatgccc ggatacatgc cgaaatgcgc gcccacattc cagccgccct
     6181 gggtggtcgt cacgtcgatc ttgaagaagc cgggcagcag gccgttgcgc accgcgctgc
     6241 tgttgccgct gcccacggtg gccaggccgc cggtcacggc agtgttggcg ccggccttct
     6301 gcgggctgtc atgcacgtag aacccgttga ccgagccgga gaacttgatc gttgccgggc
     6361 ccaccaccag gcccacgccg ctgtcggtca tcttcacgaa acggcgatca tcgatctggg
     6421 ccggggcggc cggctggcca ccgggggccg ctgctgccac cgcctctgcg gccttggcct
     6481 gatcggcctt ttcctccgcc tgcagggcgg catattcgtc gtcggtcaga atgcccttgt
     6541 catgcaggcg cttcagcagg tccgaggcgg tgctggcctc ggcgctggtg gcccccaggc
     6601 acaaagccag agcaatggcc gcgccgccaa gcagtttcgt ctttcgcatc atcgtccccc
     6661 ataaggaatg ccgtctcatg cggctgaggg gaacatgcgg cgccaggcgg gcggctaaaa
     6721 ttggactttg gccgtaatac tttgggataa ggaatttata cttaatggtt tatgcggcgg
     6781 aatgcgagcc cgcccccccg catcaggggg cgatggccac gccccacggc ccgctgccgg
     6841 ccggcacgct ggccaccacc ttcaggctgg ccacatccac cacgctcaca tcatcgctca
     6901 tgccattggc caccagcacc tgccgctcgt cagggctgag cgccagatgc cagggccgcc
     6961 cgcccaccgg cacataggcc agcaccttgc gcgtggccac atccaccagg gcaatgtggt
     7021 tggcccgccc cagcgccacc caggccttcg tgccatcatg cgtgaaacgg atgccgcagg
     7081 gcagcacctt gtaggccgaa acacccggcg gcgcgaaggg gatggtggcc acgatcttgc
     7141 ggctggccgg atccaccacc tgcaccacct gccccgcctc tgccgccacc cacagctcat
     7201 ggccatcggg cgtaaattcc acatggcgcg ggcggtgttc ggtggcggtg atgccggtgt
     7261 tggtgtgagc ggccagatcg atccaggcga tattgtgctg gtcctcgctg gtcaccacca
     7321 gccacttgcc atccgggctc tgggtaatcc cctccggctc gcggcccacc ggcacctgcc
     7381 aggccacctg cccgctggcc agatcgatcg cggtggttgc cgcatcatcc tcattggcca
     7441 caaacaggct cttgccatcg cgcgagggga agaactgctc cggatcctgg cccgatggca
     7501 gatgcgccag cagcttgccg gtgatcctgt cgcgcacttc caccgtattg tcggtgcccg
     7561 ccgcgatgaa caggcgcgcg ccatcgcgcg aaagggcaat gccgcgcggg cgctggccca
     7621 ccggccagtt ggccagcacc tggcgcgcgt cgatgtcgat caccgtcacg ctgttgccac
     7681 gctcgttcga gacataggcc gtgggcccgg ccagggccgg ccccgccagc cccgccgcca
     7741 gcacactcca cagccaccgc tgccgcacac gccttgccaa aatcgccata cgttctccca
     7801 tgccggcaaa ttatccgcgc catgccagcg gcatactgga ccaaggtact atgtttgcgc
     7861 ccgcatgcgg acagtatccc ggcggcaaga agggttctgg cagaacctgc ccggcaaggc
     7921 cacatcgagt cggccccgcg gcacatgaag ggagatacgg gtgatgagcg gcaagctggg
     7981 tcgcgcggtt ctgattggcg ccatgttgct ggccaccgcc agcatgggct cgcgcatctg
     8041 ggcgcatggc aatgtggtgc cccagccggt ggataccagc gccctgcccg atatcgaggg
     8101 cggcaacgac aattgggtgc agcacaaccc ctatcgcggc aatgccaagg cgattgccat
     8161 cggcgaatcg gcctatggcc agaactgcgc gcgctgccac gggctggatg cgatctcggg
     8221 cggcatcgcg ccggatctgc gctatctgga actgggcgac agcggcgatg aatggttcgt
     8281 ccagcgctat caccatggct ccagccacga tggcaaggtc tatatgcccc cgatgggcga
     8341 gatcctcggc cagaaggccg gctgggcgat ccgcgcctgg ctggaaacca agcacacgga
     8401 caattgagat gatcgcccgg cgcgccctgc tcggcgcttt ggccgccagc gccggcagcc
     8461 tgctggcgcc ttgcgccctg caggccgccc cgctggccaa ggtgaaggaa cagggccgcc
     8521 tccggatcgc cgtctaccag gataaccgcc cgtggtcttg ggccgatggc gcgggcctgc
     8581 atggcatcga tgtcgatctg ggcaaggcgc tggcagccca gctgggcgtg gcggccgaag
     8641 tggtcgattt catgccgggc gatgatctgg ccgccgatct gcgcaacacg gtgtggcgcg
     8701 gcgggctgct gggcttccag ccctgcgacg tgatgctgca tgtgcccttc gaccatgaat
     8761 tgcagctcgc caacgaccag gtggcgatcc tggcgcccta ctgccgcgag ggttttgcca
     8821 tgctgtgcgg cagcgatgtc gattgcgagg cgccgccgcc gcattttcgt ggcctgcgcc
     8881 tggcggccga aaccgccagc gcttccgatg cctatctgct gggcgccttt ggcggcgtgc
     8941 tgcgcggcga tgtgcaccat ttccgcaatg gctatgatgc ggccgcggcc gtgggcagcg
     9001 gccaggccga tatggccatg gccaccgccg cccaggtgga agccgccagc cacgatgcca
     9061 ccgccagcct tcaccggcgc cgcggccccc tgccggccat gtcctcgccc ggctgggatg
     9121 tggcgatggc ggtgaaggag aacagccgca cgctgggcga tgcgctggaa aacatcatcg
     9181 gcaagatggt cgccaatggc cagatggccg cgctgttcgc cgcccatggg gtcagctgga
     9241 aggccgccct ggccggctga tcgccccacc ggggccaccc ttgcgcaagc ctgccggccc
     9301 gcgccttttt ccatgcatca cgggcttttg cggatgcggc cgggcttggc ctaaggtcca
     9361 attgctgcct gcccgccgcc cgataggttc cgctggcgga gagcaacaaa agcacctggc
     9421 ggcaagcgcc gacaaagtgc cgcagtggcc gccttttccc gcctttcagg cccgcaaggg
     9481 gccggacggc acagcaggcc aaaccacaac aggccaaacc acaataggag ggtatgcatg
     9541 aaggggagtt tcaagcgcag catggcgggc tttgccacgc tggctctggc agcggcgatg
     9601 ccggcctggg ccgaaaatgg ccccaccagc caggacatcc tcaacgatgc caacacgccc
     9661 ggcgatgtgc tgagctatgg catgggcccc aaggcccagc gcttcagccc gctgaacgct
     9721 gtcaacacca gcacggtgaa gaacctggcg ccggctttcg cggcctcgct gggcggcgaa
     9781 aagcagcgcg ggcaggaagc ccagccgctg gtctatgatg gcacgatcta tgtcaccgcg
     9841 tcctattcgc gcgtgttcgc ctttgattcg cacaccggcg agaagaagtg ggaatatgac
     9901 gcccgcctgc ccgatggcat catgccctgc tgcgatgtgg tgaaccgcgg cgctgccatc
     9961 catggcgaca agatcatctt cgccacgctc gatgcccatc tcatcgcgct gaaccgccac
    10021 accggcaagg tcatctggaa ccagaagatg gccgactatt ccgccggcta ttcggccacc
    10081 gccgcgccgc tgatcgtgaa agacatggtg atctacggca attcgggcgg cgagttcggc
    10141 attgtcggcg aagtgcaggc gcgcgatgtg aacaccggcg cgctgatctg gagccggccc
    10201 accgtggaag gcaacatcgg caccctgcat ggccagccca acggcatgac cggcaagctc
    10261 aacgccacct ggcagggcga ccagtggaag accggcggcg gcgccacctg gctgggcggc
    10321 acctacgacc cggagaccaa cctcatctac atgggcaccg gcaaccccgg cccgtggaac
    10381 agccacgatc gccccggcga caacctctac accgcctcca cccttgccat cgatgccgac
    10441 accggcgtca tcaagtggca ctaccagacc accccgcacg acggctggga cttcgacggc
    10501 gtgaacgagt tcatcccgtt tgatgccacc atcaacggca aggcgatgaa gctgggcgcc
    10561 aaggccgatc gcaacggcta tttctacgtg ctggatcgca ccaacggcaa gttcatcagc
    10621 gccagcaaat tcgtgatgca gaccagctgg gccaatggca tcaacgccaa gaccggccgc
    10681 cccaacttca tcgacgagaa ccgccccggt gcgcccaatg gcgccgacaa gggcaaggtg
    10741 gtgtttgcca gcccctcgtt cctgggcggc aagaactgga tgccgatggc ctacagccag
    10801 gacaccggcc tgttctacat ccccagcaac gactggggca tggatatctg gaacgaaccg
    10861 atcgcctaca agaagggcgc ggcctatctg ggcgcgggct tcaccatcaa gccgatcgcc
    10921 gaggaccaca tcggcgcgct gcgcgcaatg gaccccaaga ccggcaagat cgtgtgggaa
    10981 tacaagaaca aggccccgct gtggggcggc gttctcacca ccggcggcgg gctggtgttc
    11041 accggcacgc ccgaaggcta tctgaaggcc ttcgacgcca agaccggcga ggagctgtgg
    11101 aagttccaga ccggctcggg cgtggtcggc tcgccgatca cctgggaaca ggatggcgaa
    11161 caatatgtcg ccgtgatgtc gggttggggc ggcgcggtgc cgctgtgggg cggcgaggtc
    11221 gccaagacct tcaaggaaat cagccagggc ggcatgctct gggtgttcaa gctgcccaag
    11281 cactgaggat ggggcacggg tggaggcggg ggcctccacc cgcccttggc gcaaccgcat
    11341 gattgcactt gcgcccctcc atgataggct gaacgggagc agcaacagga acatgacgac
    11401 gaccatggag cgggtactga ttgtcgatga ccattcactg gtccgtgatg gcatgcgcag
    11461 cctgctggaa atcagcttcc cccattgcga gatcctggaa gcgggcgccc ttgccgaagc
    11521 ggtcgccacg ctggaagacc agggcgatgt cgacatggtg ctgctcgatc tcaacattcc
    11581 cgatgtcacg cgcttttccg gcctcagcgc cctgcgtgaa acctttccca gcattccggt
    11641 ggtaatggtc tcgggcacgc tcgatcgcat caccgtgcgc gaggcgctgg ccgctggcgc
    11701 tgccggtttc gtgcccaaat cgctgaagcg cggcgagatc gtcgaagccc tgcgccaggt
    11761 gctggaaggc gagatctaca tccccgacat gttcgacgag gatgacgaga ccagccgcga
    11821 acacgccgcc atcctcaccc gcatcgaaac gctcacgccc cagcagaagg tcgtgctgcg
    11881 ccacctcgtc aacggcaagc tcaacaagca gatcgcctat gagctggatg tctcgatgac
    11941 cacggtgaag gcccatgtct cggccatcct gcagaagatg aacgcctttt cgcgcaccca
    12001 ggcagtgatc ctggccaacc gggtgggctt ccactaagcg ttttccgcgc ccggggcgtt
    12061 tgctgcgccc ggggcgtttt cccgcaaagc gccccttcag gccatcatca tccgcagcag
    12121 ggcgcgcagt tgcgccggct tcaccggctt gttcagcaag ggcaggccgg ccgccgtcaa
    12181 ccgggccttc acttcggcgg tgcggtcggc cgaaatcacc agcgccggga tctccacccc
    12241 gcattccgcc cgcagcgcgg ccaccatctc atcgcccagc tggtcgtcat ccaggtggta
    12301 atcggcgatg atgagcgccg gcggcgcctc gcccagcact tgcgcgcggg cctcctccag
    12361 cgtgcccgcc gtggccacgc catagcccca tttgcccagc agcgcctgca tcccttccag
    12421 aatggccggc tcattgtcga tgatcagcac cgtgccggtg gccgccgcca gcccccgctc
    12481 acgcgggcgg ggcagcggcg cctcttcggc cagccgcgcg ctcagcggca ccagcacggc
    12541 aaattccgat ccctcgcccg gctgcgaggc cacttccagc cggtgcccca gcatctggct
    12601 ggcacgcttg acgatggcca gccccagccc cttgccactg ggcccgcgct gggcagacag
    12661 gcggcggaat tcctcgaaaa tcacgtcgaa atgggccgga tcgatgccgc gcccggtgtc
    12721 cttcacggca atgcgcacta tcgcgccatc gctttccacc cgcaccgcca ccgagccctg
    12781 ccccgtatac cgcagcgcgt tcgacaggaa attctgcaga atgcggcgca gcatgcggaa
    12841 atcggatttc acccacaggc ccgtatcggg aatgtcgagc gccagccccg cctgcttggc
    12901 ctgggccatg aactcgatcc gcagcgcgcc cagcatgcga tccagctcga tcgcggcaat
    12961 ctcgggctgc acggcgccgg catccagccg cgaaatctcg aacaaggcct ccagcagctc
    13021 ttccaccgaa tcgagcgcca cccccgcctg gcgcaccagc gcgcgcgaag gcaccgccaa
    13081 ccgccgctcc gccagcgccg agacgaacaa tcgcgcggca ttcagcggtt gcagcagatc
    13141 atggctggcc gccgccagaa agctggtctt cgatcggcta gccagttccg ccgccgtctt
    13201 ggcggccagc agctcggcct ccatcgcccg ccgctcggcg acttctgctt ccagcgcggt
    13261 ggtgcgctcg gccacgcgcc gctccagcgt ttcggccaca tcgcgcaggc gctcctcata
    13321 ggcgatgcgc tctgtcacat catcgaagct gaaggccatg ccatcgggct ggatcggcgt
    13381 ggagcggatc tccaccaccc gctcgcccag ctggcagcgg ccggcgcggt gctggccggg
    13441 ggccatg
//
LOCUS       DASRYE010000168         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_133322,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000168 DASRYE010000000
VERSION     DASRYE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133322"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFF98_08705"
     CDS             complement(<1..258)
                     /locus_tag="VFF98_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63751.1"
                     /translation="MSRPPGRLLFAALILLVGANLRPLLASIGPLLDQIQRDTGLNDT
                     RASLLTSLPVLLMGLCLLGTGRVQAAVGRRRGIAAGLAALVF"
     gene            355..858
                     /locus_tag="VFF98_08710"
     CDS             355..858
                     /locus_tag="VFF98_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU63752.1"
                     /translation="MVMDRAEQAASQWAQERPDLDTGPMVLLGRLAEAALTTARARLN
                     PLFAEHGLQPGEFDVLATLRRSGAPHALSPTDLYEAAMISSGSMTNRIDRLEKEGLVE
                     RRPNPADRRGTLVALTAGGLRLIDAMIALHVANQHSIVSGLDAQEQEQLSRLLAKLLA
                     TAQNTGT"
     gene            complement(890..979)
                     /locus_tag="VFF98_08715"
     tRNA            complement(890..979)
                     /locus_tag="VFF98_08715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(943..945),aa:Ser,seq:tga)
     gene            complement(1076..>2319)
                     /locus_tag="VFF98_08720"
     CDS             complement(1076..>2319)
                     /locus_tag="VFF98_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZU63753.1"
                     /translation="AQRSRAPIQRLADAVAGWFVPAVLAVALLAFAGWALWGPAPRLA
                     HGVVAAVSVLIIACPCALGLATPMSIMVGIGRGAGLGVLIRNAEALERFEKVDTLVMD
                     KTGTLTEGHPALAALIPAAGFDEAALLAHAAAVEALSEHPLARAILAAARERGLALPP
                     AEGFSAPTGKGVLGVVEGRQVVIGHAAFLAEQGIDAAPLAAPADSLRREGATALFVGI
                     GGALAGLVALADPLKANAAATIAALAAEGIRVVMLTGDNRATAEAVAARLGIAEVEAG
                     ILPAGKAAVVQRLRTQGHVVAMAGDGVNDAPALAAADIGIAMGTGSDVALQSAGVTLL
                     KGDLGGILRARRLSRATMRNIRQNLFFAFVYNAAGVPVAAGLLYPLTGLRLSPEIAAA
                     AMALSSFSVVSNALRLNRARL"
BASE COUNT          389 a          821 c          813 g          296 t
ORIGIN      
        1 aaaaacgagc gctgccagcc ccgccgcgat accgcgccga cggcccaccg ccgcctgcac
       61 ccgccccgtg cccagcaggc acaggcccat cagcaaaacc ggcaggctgg tcagcaggct
      121 ggcgcgggtg tcgttgagcc cggtgtcgcg ctggatctgg tcgagcagcg gcccgatgga
      181 ggcaagcaag gggcgcaggt tggcgccgac aagcaggatc agggccgcaa acagcagccg
      241 gccgggtggt ctggacatgg cggtactccg tcggggcaag cgcccttgca gccgggcttg
      301 cccctgttgt atggctctac gtagagataa atatctctac gtcaagatag atggatggtg
      361 atggacaggg cagagcaggc agcaagccaa tgggcgcagg agcggccgga tctcgatacc
      421 gggccgatgg ttttgctggg ccgcctggcc gaggcggcgc tgacgacggc gcgggcccgc
      481 ctcaacccgc tgtttgccga gcatgggctg cagccgggcg aattcgatgt gctggccacc
      541 ttgcggcgca gcggcgcccc ccatgcgctg agcccgaccg atctctacga ggcggcgatg
      601 atttcctcgg gcagcatgac caaccggatc gaccggctgg agaaagaagg gctggtcgaa
      661 cgccgcccca atcctgccga cagacgcgga acgctggtgg cgttgacagc cggcgggctg
      721 cgcctgatcg acgcgatgat cgccctgcat gtcgccaacc agcacagcat cgtcagcggc
      781 ctcgacgcgc aagagcaaga gcagctcagc cgcttgctgg ccaaattgct ggccacggcg
      841 caaaacaccg gcacgtaaac cgcagcctgg ctgaggggcg gcagcgctgt ggcggacagg
      901 gtgggattcg aacccacggt gggctcacac ccacggcggt tttcaagacc gctgccttaa
      961 accactcggc cacctgtccg cgttccgggc tggggttggc gggcgggccc gccgggcaag
     1021 gccggaagcc cttgcgatta tcgctcctgc ggcaaaggtc aagtgcgatt ggcgtttaca
     1081 ggcgcgcacg gttcagccgc agggcattgc tgaccacgct gaaggaggac agcgccattg
     1141 ccgccgcggc gatttccggc gacagccgca gcccggtcag aggatagagc aggccggcgg
     1201 ccaccggcac cccggcggca ttgtagacaa aggcgaagaa caggttctgg cggatgttgc
     1261 gcatggtggc gcgcgagagg cggcgggcgc gcagaatgcc gcccagatcg cccttcagca
     1321 gcgtgacgcc ggcgctttgc agggccacat cgctgcccgt gcccatggca atgccgatat
     1381 cggcggcggc cagcgccggg gcatcgttca ccccgtcgcc ggccatggcc accacatggc
     1441 cttgcgtgcg caggcgctgc accacggcgg ccttgcccgc gggcagaatg ccggcttcca
     1501 cctcggcaat gcccagccgg gcggccaccg cttcggccgt ggcgcggttg tcgccggtca
     1561 gcatcaccac gcggatgccc tcggcggcca gcgcggcgat agtggcggcg gcattggcct
     1621 tcagcggatc ggccagggcg acaaggccgg ccagcgcgcc gccaatgccc acgaacagcg
     1681 ctgtcgcccc ttcgcggcgc aggctgtcgg caggggcggc gagcggggcg gcatcgatgc
     1741 cctgctcggc caggaaggcg gcatggccga tcaccacctg ccgcccctcg accaccccca
     1801 gcacgccctt gccggtgggc gcggaaaagc cctcggcagg cggcagggcc agcccgcgct
     1861 cccgcgccgc agccaggatg gcgcgcgcca gcggatgttc cgacagcgcc tccaccgccg
     1921 ccgcatgggc cagcagggcg gcctcatcga agcctgcagc ggggatcagc gcggcaaggg
     1981 ccgggtggcc ctcggtcagc gtgccggtct tgtccatcac cagcgtgtcc accttctcga
     2041 agcgttccag cgcctcggca ttgcggatca gcacgcccag cccggcgccc cggccaatgc
     2101 ccaccatgat cgacatgggc gtggccagcc ccagcgcgca ggggcaggcg atgatcagca
     2161 ccgacaccgc cgctaccacg ccatgcgcga ggcgcggcgc cggcccccac agcgcccagc
     2221 cggcaaaggc cagcagcgcc accgccagca ctgccggcac gaaccagccg gccaccgcat
     2281 cggccaggcg ctggatcggc gcgcggctgc gctgggctt
//
LOCUS       DASRYE010000169        19808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_9755,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000169 DASRYE010000000
VERSION     DASRYE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19808
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9755"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFF98_08725"
     CDS             complement(<1..686)
                     /locus_tag="VFF98_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZU63754.1"
                     /translation="MTVTKTPYDMAELRREALLGGAGETVERPLPQIDMRDFAARKAD
                     IAEEIWAASADIGFFQLVNHGIPEDAIAQAFALAERFFALPQDVKAHRTMVPGTNAGW
                     EFKTQVRPSAGTPDNKESYQITLPRMAGLWPEEAELAGFRTRMLAFERMNWELGMRVL
                     SCFALKLGFAEDFFTRAHDPASADYQSTLRLVHYMSMEGADPADFDLWRAAAHSDFDC
                     LTLLHQKPGQG"
     gene            complement(751..2112)
                     /locus_tag="VFF98_08730"
     CDS             complement(751..2112)
                     /locus_tag="VFF98_08730"
                     /inference="COORDINATES: protein motif:HMM:NF009059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU63755.1"
                     /translation="MSDLVIRSALGLFTALDGPAARAQGDIRIRDGRILAIGEIPPLP
                     GDRIIDASGCIVTPGLVNTHHHLFQSVLKGIAAGINVPLEPWLRMVPYAHWHRLDGEA
                     LAVAAELGMVELLLQGCTTLADHHYLFDAAQNYDAAQILFETAERLGLRFVLARGGSA
                     ISRSFDNDDIVPMPVESVEQMLSATEALAHRYHDPAADGMARVALAPTTPFWSLPAGS
                     LPEVIAATRAMGLRLHSHLSETGTYVDYALASFGQRPIPYVASQGWVGEDVWYAHLVH
                     LDADEVAILAETGTGMAHCPQSNGRLGSGIAPASALAARGGRVSLGVDGAASNEAADL
                     VSEMHVGWMFQRAAHGAGAATCEEVLRWATAGGADVLGLAAIGTLAPGQAADIAIFDL
                     AHPRYAGLHDPLIAPVASGGAAHLRHVLVGGRQVVQDGAIPGLDLPRLMARAAATVQR
                     MAG"
     gene            complement(2245..2961)
                     /locus_tag="VFF98_08735"
     CDS             complement(2245..2961)
                     /locus_tag="VFF98_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63756.1"
                     /translation="MFDVPTLKIKARSGIYATGQERVLQILETAVDILIHDGFPAVTL
                     REIARRMGIRVSAISHYYSSREDLILDVLSGVLSSYEEFFETFRTPSDEPPEARLRSF
                     IAAVLDDILTLKTTRLFPELWALASRDDNVARMVDAIYIRSRVIQKRFIAALNPGLDA
                     RTLENLALYITASLEGLTVFAGHGKPWAGEMEHLKRIACDTLIAAVKAARPDPAADPH
                     WQAPTLLDTESYGRLIALDD"
     gene            3266..5662
                     /locus_tag="VFF98_08740"
     CDS             3266..5662
                     /locus_tag="VFF98_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63757.1"
                     /translation="MNSSRILFASAGRLALAAVLGAGIAGVAHAQDAAPAAPAKADAI
                     GEIVVTAQKRQTSADKTPISITAVSGADLAARGVTSFADLAAGTPGVSIKSMGPGQTE
                     FEMRGMTSSGGNSPTVGFYLDDIPLTSPASAQNGKVVIDPTLYDLSQVEVLRGPQGTL
                     YGSSSMGGTVKLVTNKPKLGKWEASAELTGSGTQGGGFNHAENAMLNIPITPTLALRA
                     VVSQSNTSGFIDRVVVANGDFPQSTNGGVTRGNVASAPVEKVYHGSNAQNVIGTRVSL
                     LWKPVDELTITPTFFYQRTHANGPSTYDSDPGTMAHYQPFDIAEPYTDEIKIGAATIN
                     YHFPTFDVTSATSYWSRKSTMVQDNSENLPSCGGGGFCVNSGDYTYYGSGGTGAIAAY
                     ETDPSQQFAEELRLTSTGKGRLKWLVGAYFASFKSSWQLETDVANPEVFGSPTPKIFS
                     LNEPTRIKQYALFGEANYEVTKNLHVTVGLRGYHYDTTLDMVTGGYGSPTGDGTYITQ
                     HVVQKSTGVNPKFDISYQATPNTLVYVTVARGFRPGGGNQPLPSDGTQPEGTFNRGMY
                     DALVNMGYASGKAPVAYGPDHLWSYELGEKAKLFDRHLRLNASIYYENWQAIQMELLP
                     EGYPLFDNAASAKIYGGEVEAQAVLTSHLSVGGQIGYTHATLDQAAHGLVDGQRLPDV
                     PKWSGGGNISYSRALDTVHDFTFRVDDTYVGDRVGLATYQGSVNNPNTPLPAYNLVNL
                     RTGVASKAGWSVAFFITNLTNKHAYLENAAQLGLPNASYNRVVTNQPRTFGVDLSWHL
                     "
     gene            5838..6746
                     /locus_tag="VFF98_08745"
     CDS             5838..6746
                     /locus_tag="VFF98_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZU63758.1"
                     /translation="MKILAMQASPHALAGRDAEGVSFARFAAEARAALAAHPDTDLLL
                     FPELHLFHPAAADLAAANRALRAAAQPLDGPLDGSLDRALAALAAELGVALLPGTLAE
                     AGPEGQLFNTARVYGADGRVLATYRKIFPWRPSEPYDPGAEWVVFDLPGKGRLGLTIC
                     YDAWFPEATRQVAWLGAELVLNLVKTTTPDRAQELVLARANAIVNQCFLVSLNCAGPV
                     GMGQSLVVGPEGEVIAEAGVAPQAIAVTLDLDRAQHVRQHGTCGENRLWAQLRPGEAP
                     IALPAYAGRMDPADWNPQTRLKDPQA"
     gene            6743..8047
                     /locus_tag="VFF98_08750"
     CDS             6743..8047
                     /locus_tag="VFF98_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZU63759.1"
                     /translation="MSQPRPHLGLPALVLFGLAYLAPLIVLGTFGVVAQASHGATAGS
                     YVLALAAMLFTAASYGRMAALHPVAGSAYAYVCHAIDRRVGFMVGWAVTLDYLFLPMV
                     IWLIGAAYLHDSFPALPMPALVLGFICITTFLNILGFRLAKGVNGLLMAVQAVVIVLF
                     VALAAAFVGHGGPLLQSQPFMNPASGIGAIAAGAAIAAYSFLGFDAVTTLAEECHEPR
                     RLLPGAIWLTALVGGAIFVIVSYIAQLAHPGFAFHDPAAAANEIARAIGGRWFEGLFL
                     AGLVVAQFASGIAAQAAGARLIHAMARDGVLPRRLLGRLHPRFGTPAAAVLLTGVIGL
                     LALGLTVESSTSFINFGAFTAFSAVNLSVIATAIRQGRRGILGWYLAPAAGMGVDMWL
                     LTRLEPAAIHLGLAWLAVGLVVLAWLTRGFRRPPPALHMEEH"
     gene            complement(8061..8996)
                     /locus_tag="VFF98_08755"
     CDS             complement(8061..8996)
                     /locus_tag="VFF98_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU63760.1"
                     /translation="MSASDRLPPFAALRAFAAVGEVGGIRKAADALSISHAIVSRHIS
                     ALEAELGMLLLDRRSGQLTEAGQAYHARISAAIGEMEEATRALRASRGGVLTIWCSPG
                     LSLLWLARRLPDFAARAQGALGRPVIDLRSTDSEPHFERNEADGDIRYLFDNDPATSL
                     RGVRTDELARPPVFPVAAPELLARLPRPVATVDELVNLPLIQENSASEWAGWLAAQGR
                     DATRLPPPVARYGQAHLTLAAARAGQGLALSNHFLVAEDLASGRLVQVEPTAEPARAV
                     TLGAYAFRCARARWSDPLLVRFRQWLHRKIAEEGI"
     gene            9101..10384
                     /gene="gabT"
                     /locus_tag="VFF98_08760"
     CDS             9101..10384
                     /gene="gabT"
                     /locus_tag="VFF98_08760"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZU63761.1"
                     /translation="MSNQSLLDRRIAAVPRGVGNATNVYAARAENAEIWDVEGKRYVD
                     FAGGIAVLNVGHRHPKVMAAVEKQLAAYTHTAFQVMAYEPYIELAEKLNALAPIEGAA
                     KTIFFTTGAEATENAVKVARAATGRPGIIAFAGGFHGRTALSGAMTGKVNPYKRGLGP
                     FPSHIYHVPLPSATNGITVAESLRALDFLFAADIEPEQIAAFIVEPVQGEGGFHTPDP
                     ALFEALEKLREEYGILIIADEVQSGFARTGKMFGIEHQSVKPDLISVAKSLAGGFPLS
                     GLIGRADLMDVIAPGGLGGTYAGSPVACAAALAVLEVIEEEKLLDRANAVGARIRAAV
                     QAAAARNDTLGIANLRGPGAMIGFDVVDGAGKPDAAAAKQVVTRALDKGLVVLTCGTH
                     GAGIRILVPLTASDALIDEGLAILTEALVNEEVAA"
     gene            10384..11859
                     /locus_tag="VFF98_08765"
     CDS             10384..11859
                     /locus_tag="VFF98_08765"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZU63762.1"
                     /translation="MATLAPSSLVLSEPDLLRDAAFVGGTWLAGEETIAVTNPADGSL
                     VGTVPNLGAAETTLAVDAAARAQALWAKETGKARGQVLARWAELMATHADDLARLMTA
                     EQGKPLAEAKGEVAYAASFLEWFAGEARRVNGEILTPHAADRRLLVRKEPVGVVAAVT
                     PWNFPLAMITRKAGPALAAGCAVVLKPSELTPLSALALARLAEQAGVPAGVFNVVTGD
                     AAPIGDVLTGDKRIRKFTFTGSTRVGKLLAARCMDTVKRVSLELGGNAPFIVFDDADL
                     DAAVEGAMASKFRNAGQTCVCANRLLVQSGIYDAFASKLSAKVRALVVGPGLAGPSDQ
                     GPLIDGRAVAKAEAHVTDALARGGRLLVGGRAKPGRGSFFEPTVIAEVPGDALLCREE
                     TFGPVAGLVRFETEEEAVRLANDTDAGLAAYVFTRDIDRAWRVPEALQYGMVGLNTGL
                     ISTEVAPFGGVKESGMGREGSHHGMDDYLSLKLVCQAVSRG"
     gene            complement(11927..12544)
                     /locus_tag="VFF98_08770"
     CDS             complement(11927..12544)
                     /locus_tag="VFF98_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZU63763.1"
                     /translation="MYIPPPYQEHRQEVLVGAIRQRGFGTLASFNAGQLHVSHLPFVL
                     AGAGEELTLIAHLARANPQWRGLAGTEAAATFLIDDAYISPGWYPSKQDAGRVVPTWN
                     YIAVEARGQMEVVEDAEGLLALLEPLTAAHENHRPRPWTVAEAPADYLAALLKGIVGI
                     RMVAPVLTGAWKLDQKKAAADRLGAAAGLRRDEGAEGIAGWMEQV"
     gene            complement(12574..13737)
                     /locus_tag="VFF98_08775"
     CDS             complement(12574..13737)
                     /locus_tag="VFF98_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZU63764.1"
                     /translation="MTHTIALPRLMRIGAGASGELAAVLASLGLAHPLIVTDRYLADH
                     GLADRLVAGLAAAGIPSRVFADTVPEPTTASIAAGLVCLAAAPHDCVVGFGGGSPIDS
                     AKAIAVLGPLGGTAQDYKAPHLQDAPGLPIIAIPTTAGTGSEATRFTIITDETRDEKM
                     LCAGLAYLPVAALVDYELTLSMPPRLTADTGIDALTHAIEAYVSRRAHPFSDQAALAA
                     MAAIAPNLRRVWADPANRPAREAMMFGATMAGIAFSNASVALVHGMSRPIGAHFHVPH
                     GLSNAMLLPAITAFSAPAALERYAACAVAMGLDVAGLGQQAAVGVLVEELRQLNADLA
                     VPSPARHGIDKARWHALTPLMAEQALASGSPANNPRLASAAEIEALYAAVWDG"
     gene            13913..15163
                     /locus_tag="VFF98_08780"
     CDS             13913..15163
                     /locus_tag="VFF98_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HZU63765.1"
                     /translation="MHEHPLRRQIVGEMHLRRWPAVAAPATIVQMLRQVAPEQRAAEA
                     AHLAQVAAALPEGARHAMGQLAPGLAYVWERHAEASGITLFAQEGADPAAGLAWLAGF
                     PGAVVRATRIRVVSGEAQVAAALAGAGFLASDLVSCGIGPEGCRLWSDFRIGADGFGQ
                     LVVDVPEAPAQAAARQGHDVARLLQTLQDLGNYRNLALLGLPLARAHWPRLDGVEEAL
                     RQLAGDVGRADLTDDALLEKVCAISLELMSIGAETRYRLSATRAYARLVEERLGQLAV
                     RPIAGYPSLEDFTHRRFLPAVRTCSAFDRRLGELTARGAQFTALLRTRVETRIENQNG
                     QLLRSMEQSASLQLRLQQLVEGLSVVALSYYALALVGYVLKGVEEVAPHFPAVAAEGA
                     LVPVVVACVWLGLHRMKARLLGGH"
     gene            complement(15397..17745)
                     /locus_tag="VFF98_08785"
     CDS             complement(15397..17745)
                     /locus_tag="VFF98_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63766.1"
                     /translation="MTQDQCAQRRRATGSALALAIGLCAAALAGNAQAEEGAPTTPIA
                     KGTDTSIADIVVTARKRAEPLQRTPLAVSALTSEQIEAKAPQNITDLGGSMPNVRIDS
                     LGSQGRAGMLAIRGVNYARSDQAGDPSTAFYIDGLYQPRSTLNVLDMFDIESVEVLRG
                     PQGTLFGRNAFAGAVNIQSKRPTQEFGGQVEGRIGNYGRWETRAAVNVPIVQDVLAMR
                     ASLLVTKSDGYYHSIQENGKPLGGDDNITGRISLLYTPSPDWNIFFKYEHVRDRSQPT
                     PAQNASTTPAMASSALPAQLFGSIAGDPPNLYNYPRYWTNVNIRPGQDSFVNQDNVAL
                     NITRNIEGGSINLITGYQYSSDALFTDPVGGKIAYLTNYYRTQLKNFSQEVRGIKELN
                     KTFQLLVGAYYAHDDLTYGNITYSEYAPLTAANSQTNVMQKRDSWAVFGELEVRPMRN
                     VRLNFGGREMGETKDFYFAYQSRDPTTVAAAVGLSPGVFLQVGPYGEAKKSWTNFSPK
                     ISVDWRPTSEMMLYASWTKGFKSGGFSAISTSLAGAGPYDPERIETWEAGLKSFWFHH
                     RLKANITLFWNNLSDLQRSVNFINSQGQSSNNVFNAASAVTKGVELELEARLTRGLNI
                     TGSAGFLDAHYKSFCAAGFSATQILGQPTAQVCNTTTGALDWSGIPLYNAPKFQGSIG
                     VDYKLDLHHGEAVRFNSELNYTSSLFTNDTPWPISMRRPMVVVDAGLRWNSKGDRYYA
                     AFNVKNLFNRIDTQQAIRASSILTVFNYTAPRTYVATLGVKF"
     gene            complement(17907..18632)
                     /locus_tag="VFF98_08790"
     CDS             complement(17907..18632)
                     /locus_tag="VFF98_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63767.1"
                     /translation="MPTRSKPAPPAPATTPARRSRRPSPGQLERREATRRKLLEAAGQ
                     MIGKYGFAGCTIARVTARARIAHGTFYLHFQSQQEMFEELLREMSLRMLEHVRDAVHE
                     CGSLVELERRGLEANHDFFCRYPFMARLTQEAELYSPVSYTAHIDAMANGYVRALARK
                     RAGTVLEGWSHAQLEQLAVMLIGARSQIMRRYGVAGHKVVPLSPDVIDTYIRFISNGI
                     LAGLDKPAADRTEGAQAPTGERD"
     gene            19048..19623
                     /locus_tag="VFF98_08795"
     CDS             19048..19623
                     /locus_tag="VFF98_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZU63768.1"
                     /translation="MAHSTTGSGAPAPGTPKDESGLGLAFGTYEEALGWIGRATEPAT
                     GEVAVNTPMIQLYCSAVQDANPTYWDEAFAQEHWGSMVAPPGMLQTWTIPMQWRPEGA
                     RTVSVMCAKVPLPGDKPINVSTDFEFFEPVRVGDRLTMTDRLEAISPEKTTKVGTGHF
                     ITSVTEYRNQAGVLLARSTNVLFRYLSGEAA"
     gene            19620..>19808
                     /locus_tag="VFF98_08800"
     CDS             19620..>19808
                     /locus_tag="VFF98_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63769.1"
                     /translation="MSAAARQICWQDVDEATPLPVLVLDVTYKMAILHVAGGWDYMPG
                     HHDPEFARRQGAPTVFLNT"
BASE COUNT         3113 a         6476 c         6920 g         3299 t
ORIGIN      
        1 ccctggcccg gcttctggtg cagcagggtg aggcaatcga aatcgctgtg ggcggcggcg
       61 cgccacaggt cgaaatcggc cggatcggcg ccttccatgc tcatgtaatg caccaggcgc
      121 agcgtgctct gatagtctgc cgaggcggga tcgtgggcgc gggtgaagaa atcctcggca
      181 aagcccagct tcagcgcaaa gcaggaaagc acgcgcatcc ccagctccca gttcatccgt
      241 tcaaaggcca gcatgcgggt gcggaaaccg gccagctcgg cttcctcggg ccacaggccg
      301 gccatgcgcg gcagggtgat ctgatagctt tccttattgt cgggcgtgcc ggcagagggg
      361 cgcacctggg tcttgaattc ccagccggca ttggtgccgg gcaccatggt gcggtgcgcc
      421 ttcacgtcct gcggcagggc gaagaagcgc tcggccaggg caaaggcctg ggcgatggcg
      481 tcttcgggga tgccgtggtt gaccagctgg aagaagccga tatcggccga ggccgcccag
      541 atctcctcgg cgatgtcggc cttgcgggcg gcaaaatcgc gcatgtcgat ctgcggcagc
      601 ggccgctcca ccgtctcgcc ggcgccgccc agcagggctt cgcggcgcaa ttcggccata
      661 tcgtaggggg ttttggtcac agtcatgctc cttggatggg ggagcaatgt ccaggcttgg
      721 cgccagaccg cttctactcc gcgtgaaaac tcagccggcc atgcgctgca ccgtggcggc
      781 cgcccgcgcc atcagccgag gcagatccag gccaggaatg gcgccatcct gcaccacctg
      841 ccgcccgccc acaagcacat ggcgcagatg cgccgcgccg ccgctggcca ccggcgcgat
      901 cagcggatca tgcagcccgg catagcgcgg gtgcgccaga tcgaagatgg cgatatcggc
      961 cgcctggccc ggcgccagcg tgccgatggc ggcaaggccc agcacatcgg cgccgcccgc
     1021 cgtggcccag cgcagcacct cctcgcaagt ggccgcgccc gcgccatggg cggcgcgctg
     1081 gaacatccag ccgacatgca tttccgagac gagatcggcc gcctcgttgg aagcggcgcc
     1141 atccaccccc agcgacaccc gcccgccgcg cgccgctagc gccgaggccg gcgcaatgcc
     1201 agagcccagc cggccgttgc tttgcgggca atgggccatg ccggtgccgg tttcggccag
     1261 gatcgccact tcatcggcat cgaggtggac cagatgggca taccacacat cctcgcccac
     1321 ccagccctgg gaggccacat aggggatcgg cctttgcccg aagctggcca gcgcataatc
     1381 cacataggtg ccggtttcgg agaggtggct gtggagcctg aggcccatcg cgcgggtggc
     1441 ggcaatcact tcgggcagcg atcctgccgg cagcgaccag aagggcgtgg tgggcgccag
     1501 cgccacgcgc gccatgccat cggccgccgg atcgtgatag cggtgggcca gcgcttcggt
     1561 ggcgctcagc atctgttcca cgctttccac cggcatgggc acgatgtcgt cattgtcgaa
     1621 gctgcgggaa atggcgctgc cgccgcgcgc cagcacaaag cgcaggccca gccgttctgc
     1681 cgtttcgaac aggatctggg cggcatcgta attctgcgcg gcatcgaaga ggtagtggtg
     1741 gtcggccagg gtggtgcagc cctgcagcag cagttccacc atgcccagtt cggcggccac
     1801 cgccagcgcc tcgccatcca gccggtgcca atgggcatag ggcaccatgc gcagccaggg
     1861 ttccagcggc acgttgatgc ccgcggcaat gcctttcagc acgctctgga acaggtggtg
     1921 gtgggtgttg acgaggccgg gcgtgacgat gcagccgctg gcatcaatga tgcggtcccc
     1981 cggcagcggc gggatctcgc caatggccag gatacggcca tcgcggatgc ggatatcgcc
     2041 ttgcgcgcgg gcggcggggc catccagcgc ggtgaacagg ccaagggcgg agcggatgac
     2101 aagatcgctc atgcggcggg ctttgcggcg gggtggggga ggggggcgat ggacatgatg
     2161 gcgcgcaatg tgccgcgcct gcacagcttt ggcaaacgtt ttccatcacc cctgggcaag
     2221 ctgtaagccc ggtcgcgggc cggctcagtc gtccagcgcg atcagccggc catagctttc
     2281 ggtatcaagc agggtggggg cctgccagtg gggatcggcg gcagggtctg gccgggcggc
     2341 tttcaccgcg gcgatcagcg tatcgcaggc gatccgcttc aggtgctcca tctcgccggc
     2401 ccagggcttg ccatggccgg caaacaccgt cagcccttcg aggctggcgg tgatgtagag
     2461 cgccagattt tccagcgtgc gcgcatccag ccccgggttc agcgcggcga tgaagcgctt
     2521 ctggatcacc cgcgagcgga tgtagatcgc atccaccatg cgcgccacat tgtcgtcgcg
     2581 gctggccagc gcccacagtt cggggaacag gcgggtggtc ttcagcgtca ggatatcgtc
     2641 gagcacggcg gcgatgaagg agcgcaggcg ggcctcgggc ggctcgtcgg agggcgtgcg
     2701 gaaggtttcg aaaaactcct cataggagga cagcacgccc gagagcacat cgagaatcag
     2761 atcctcgcgg ctggaataat aatggctgat ggcgctgacg cggatgccca tgcggcgggc
     2821 gatctctcgc agggtgacgg cgggaaagcc atcatggata aggatatcta cggcggtttc
     2881 gagaatttgc agcacgcgtt cctgcccggt cgcgtagatc ccgctgcgcg ccttgatctt
     2941 cagggtgggc acatcgaaca tattgattcc gatcggggcc acagggccgg atgatggttg
     3001 cttgcgccct gttctaggca ggctttgggc gggctgtcat cccccctgtt gccgctttgt
     3061 cacacaaatt gagcataagt tcaactttgg tctaatgtac aaagttgagc gcttgaccgg
     3121 tttttgtcgc tgcggcatgt ttcgtgtgtc agccggcaag cagggggcgt ggcgaaaagc
     3181 cactgccatt gccggaaggc caagcgggca aacagaacca aaccgcaggc aggaatttcc
     3241 accattcatg agggatcaaa gggacatgaa ctctagccgt attctgtttg ccagcgccgg
     3301 gcgcctggcc ctggccgctg tgctgggcgc gggcattgcc ggcgtggccc atgcgcagga
     3361 cgcagcgccc gccgccccgg ccaaggccga tgccatcggc gagatcgtgg tgaccgcgca
     3421 aaagcgccag accagcgccg acaagacccc gatcagcatc accgccgttt cgggcgcaga
     3481 ccttgccgcc cgcggcgtga ccagctttgc cgatctggcg gccggcacgc cgggcgtttc
     3541 catcaagtcg atgggccctg gccagaccga attcgaaatg cgcggcatga cctcgtctgg
     3601 cggcaacagc cccacggtcg gcttctatct ggatgatatc ccgctcacct cgcctgcctc
     3661 ggcgcagaac ggcaaggtgg tgatcgatcc caccttgtat gacctttcgc aggtggaagt
     3721 gctgcgcggc ccgcagggca cgctgtatgg ctcgtcctcg atgggcggca cggtgaagct
     3781 ggtgaccaac aagcccaagc tgggcaagtg ggaagccagc gccgagctga ccggttcggg
     3841 cacccagggc ggcggcttca accatgccga aaacgccatg ctgaacatcc ccatcacgcc
     3901 cacgctggcg ctgcgcgcgg tggtcagcca gtcgaacacc tcgggcttta tcgaccgtgt
     3961 ggtggtggcc aatggcgact tcccgcaatc gaccaacggc ggcgtgacgc gcggcaatgt
     4021 cgccagcgcg ccggtggaga aggtctatca cggctccaat gcccagaacg tgatcggcac
     4081 ccgcgtcagc ctgctgtgga agccggtgga cgagctgacc atcaccccca ccttcttcta
     4141 ccagcgcacc cacgccaacg gccccagcac ctacgacagc gatccgggca cgatggcgca
     4201 ttaccagccc ttcgacatcg ccgagcccta taccgacgag atcaagatcg gtgcggccac
     4261 catcaactat cacttcccca ccttcgatgt gacctcggcc acgtcctact ggtcgcgcaa
     4321 gtccaccatg gtgcaggaca attcggaaaa cctgccgtcc tgcggcggcg gcggcttctg
     4381 cgtgaacagc ggtgactaca cctattatgg cagcggcggc accggcgcca tcgccgccta
     4441 cgagaccgac ccctcgcagc agttcgccga ggaactgcgc ctcacctcca ccggcaaggg
     4501 ccgcctgaag tggctggtgg gtgcctattt cgccagcttc aagtcgagct ggcagctgga
     4561 aaccgacgtg gccaacccgg aggtcttcgg ttcgcccacg cccaagatct tcagcctgaa
     4621 cgagccgacc cgcatcaagc agtatgcgct gttcggcgag gcgaactacg aggtgacgaa
     4681 gaacctgcat gtcacggtgg gcctgcgcgg ctatcactat gacaccacgc tcgacatggt
     4741 gaccggcggc tatggctcgc ccaccggcga tggcacctac atcacccagc atgtggtgca
     4801 gaagagcacc ggcgtgaacc ccaagttcga catctcctat caggccacgc ccaacaccct
     4861 cgtctatgtg acggtggcgc gcggcttccg cccgggcggt ggcaaccagc cgctgccttc
     4921 ggatggcacc cagccggaag gcaccttcaa ccgcggcatg tatgatgcgc tggtgaacat
     4981 gggctatgcc agcggcaagg cgccggtggc ctatgggccg gatcatctgt ggagctatga
     5041 gctgggcgag aaggccaagc tgttcgacag gcacctgcgc ctcaacgcca gcatctacta
     5101 cgagaactgg caggccatcc agatggagct gctgcccgag ggctatccgc tgttcgacaa
     5161 cgccgccagc gccaagatct atggcggcga agtggaagcg caggcggtgc tgaccagcca
     5221 cctgtcggtg ggcggccaga tcggctacac ccatgccacc ctcgatcagg ccgcccatgg
     5281 cctcgtcgat ggccagcgcc tgccggatgt gcccaagtgg tcgggcggcg gcaacatcag
     5341 ctacagccgc gcgctggaca ccgtgcatga cttcaccttc cgcgtggacg acacctatgt
     5401 gggcgaccgc gtgggcctgg ccacctatca gggctcggtg aacaacccga acacgccgct
     5461 gccggcgtat aatctggtga acctgcgcac cggcgttgcc tcgaaggcgg gctggagcgt
     5521 ggccttcttc atcaccaacc tcaccaacaa gcacgcttat ctggaaaatg cggcgcagct
     5581 gggcctgccc aatgcctcgt acaaccgggt ggtgaccaac cagccgcgca ccttcggcgt
     5641 ggatctgagc tggcacctct aagccttggc aaggcgccgg agggccgggc gaaaaggccc
     5701 gcccccggcg cccgtccttt tcctatcctg ccgcctggct ggtggcagcg gggggcatcc
     5761 tgccttgcag ggccctgtcg ccgctgccag ccctttgtgc ggccctgcaa cgggatgccc
     5821 agcccttggc gcgttgtgtg aagattctgg ccatgcaggc cagcccccat gcactggccg
     5881 ggcgcgatgc cgaaggcgtg tccttcgcgc gtttcgccgc agaggcacgc gcggcgcttg
     5941 ccgcccatcc cgataccgat ctgctgctgt ttcccgagct gcacctgttt catccggccg
     6001 ccgctgatct ggccgctgcc aacagggccc tgcgcgccgc cgcccaaccg ctggatggcc
     6061 cgctggatgg ctcgttggac cgggcccttg ccgcccttgc cgccgagctg ggcgtggcgc
     6121 tgctgcccgg cacgctggcc gaggcgggcc ccgagggcca gctgttcaac accgcccgcg
     6181 tctatggcgc cgatggccgc gtgctggcca cctatcgcaa gatcttcccc tggcgcccca
     6241 gcgagcccta tgatccgggc gccgaatggg tggtgttcga cctgcccggc aagggccggc
     6301 tgggcctcac catctgctat gacgcctggt tccccgaggc cacgcgccag gtggcctggc
     6361 tgggcgcgga actggtgctc aacctcgtca agaccaccac gcccgaccgc gcgcaggaac
     6421 tggtgctggc ccgcgccaat gccatcgtca accagtgctt cctcgtctcg ctcaattgcg
     6481 cggggccggt gggcatgggg caaagcctcg tcgtcgggcc ggagggcgag gtgatcgccg
     6541 aggcaggcgt tgccccgcag gcgatcgccg ttaccctcga tctcgaccgg gcgcagcatg
     6601 tgcgccagca tggcacttgc ggcgagaacc gcctgtgggc acagctgcgc cccggcgagg
     6661 cgccgattgc cctgcccgcc tatgccggcc gcatggaccc ggccgactgg aacccccaaa
     6721 cccgccttaa ggatccccaa gcatgagcca gccccgcccc catctcggcc tgccggcgct
     6781 ggtgctgttt ggcctggcct atcttgcccc gctgatcgtg ctgggcacgt tcggcgtggt
     6841 ggcgcaggcc tcgcacgggg ccacggccgg cagctatgtg ctggcgctgg ccgcgatgct
     6901 gttcaccgcc gcttcctatg ggcggatggc cgcgctgcat ccggtggcgg gctctgccta
     6961 tgcctatgtg tgccatgcga tcgaccggcg cgtgggcttc atggtcggct gggcggtgac
     7021 gctggactat ctcttcctgc cgatggtgat ctggctgatc ggcgctgcct atctgcacga
     7081 cagtttcccc gccctgccga tgccggcgct ggtgctgggc ttcatctgca tcaccacttt
     7141 cctcaacatt ctgggcttcc gcctggccaa gggcgtgaat ggcctgctga tggcggtgca
     7201 ggcggtggtg atcgtgctgt tcgtggcgct ggcggcggcg tttgtcggcc atggcgggcc
     7261 gctgctgcag agccagccgt tcatgaaccc ggctagcggc atcggcgcca ttgccgccgg
     7321 cgctgccatt gccgcttatt ccttcctcgg cttcgatgcg gtgacgacgc tggccgagga
     7381 atgccacgag ccgcgccgcc tgctgcccgg cgccatctgg ctgacagcgc tggtgggcgg
     7441 ggcgatcttc gtgatcgtct cctatatcgc ccagctggcc caccccggct ttgccttcca
     7501 cgatccggca gcggccgcca acgagatcgc ccgggcgatc ggcgggcgct ggtttgaagg
     7561 gctgttcctg gccgggctgg tggtggcgca atttgccagc ggcattgccg cccaggccgc
     7621 cggcgcccgc ctgatccacg ccatggcgcg cgatggcgtg ctgccgcgcc gcctgctggg
     7681 ccggctgcac ccgcgctttg gcacgccggc ggcggccgtg ctgctgaccg gggtgattgg
     7741 ccttttggcc cttggcctca cggtggaaag ctccacctcc ttcatcaatt ttggcgcctt
     7801 cacggccttt tccgccgtga acctctcggt gatcgccacg gccatccgtc agggccggcg
     7861 ggggatcctg gggtggtatc tggcgcccgc cgccggcatg ggtgtggaca tgtggctgtt
     7921 gaccaggctc gagccggcgg cgatccatct ggggctggcg tggctggcgg tggggctggt
     7981 ggtgctggcc tggctgacgc ggggctttcg ccgcccgccg ccggcgctgc acatggaaga
     8041 gcattgagcc cgcgaggcgc ctagatccct tcctcggcga tcttgcgatg cagccactgg
     8101 cgaaagcgca ccagcagcgg atcggaccag cgcgcccgcg cacagcggaa ggcataggcg
     8161 cccagcgtga cggcgcgggc cggctctgcg gtgggttcca cttgcaccag ccggccgctg
     8221 gcgaggtctt ctgccaccag aaaatggttg gaaagggcca gcccctggcc cgcgcgcgcg
     8281 gccgccagcg tgagatgcgc ctggccatag cgggccaccg gcggcggcag gcgggtggca
     8341 tcgcgccctt gcgcggccag ccagccggcc cattccgaag cggaattttc ctggatcagc
     8401 ggcaggttca ccagctcgtc gacagtggcc accgggcggg gcaggcgggc cagcaattcc
     8461 ggcgcggcca cggggaacac cggcgggcgg gccagctcgt ctgtccgcac gccgcgcagg
     8521 gatgtggcgg ggtcgttgtc gaacaggtag cggatgtcgc catcggcctc gttgcgctcg
     8581 aaatgcggct cgctgtcggt cgatcgcagg tcgatcaccg ggcggcccag cgccccttgc
     8641 gcgcgggcgg caaaatcggg cagccgccgc gccagccaca gcagcgacaa gcccggcgag
     8701 caccagatgg tgagcacccc gccgcggctg gcgcgcaggg ccctggtcgc ttcctccatc
     8761 tcgccgatcg ccgcgctgat gcgggcgtga taggcttggc ccgcctcggt caactggccc
     8821 gagcggcgat cgagcagcag catgcccagc tctgcctcca gcgccgagat atgccggctg
     8881 acgatggcat ggctgatcga cagcgcatcg gccgccttgc ggatgccgcc cacctcgccc
     8941 acggcggcaa aggcacgcag ggcggcaaag ggcggcaggc gatcggatgc actcatgagt
     9001 ggtaacctac agtcaccaca ggcggcgcgc aagtcgcctg tttcggggat gtgcggtgcc
     9061 ctatggtcaa cacaaagccc aaacaaggaa cctgcgcgag atgtctaacc agtccctgct
     9121 cgatcgccgt attgccgctg tgccgcgcgg tgtgggcaat gccaccaacg tctatgccgc
     9181 tcgcgccgaa aacgccgaga tctgggatgt ggaaggcaag cgctatgtcg attttgccgg
     9241 cggcattgcc gtgctcaacg tgggccaccg ccaccccaag gtgatggccg cggtggaaaa
     9301 gcagctggcc gcctacaccc acaccgcctt ccaggtgatg gcctatgagc cttatatcga
     9361 gctggccgag aagctgaacg cgctggcgcc gatcgagggc gcggccaaga cgatcttctt
     9421 cactaccggc gccgaggcca ccgaaaacgc cgtgaaggtg gcgcgcgccg ccaccggccg
     9481 gccgggcatc attgcctttg ccggcggttt ccatggccgc acggcgctgt cgggcgcgat
     9541 gaccggcaag gtcaacccct acaagcgcgg cctcgggccc ttcccctcgc atatctacca
     9601 cgtgccgctg ccctcggcga cgaacggcat caccgtggcg gaaagcctgc gcgcgctgga
     9661 tttcctcttc gccgccgata tcgagcccga gcagatcgcc gctttcatcg tcgagccggt
     9721 gcagggcgag gggggcttcc acacgcccga tccggccctg ttcgaggcgc tggagaagct
     9781 gcgtgaggaa tatggcatcc tcatcatcgc cgatgaggtg cagtcgggct ttgcgcgcac
     9841 cggcaagatg ttcggcatcg agcaccagag cgtgaagccg gatctcatct cggtggccaa
     9901 gtcgctggcg ggcgggttcc cgctgtcggg cctgatcggc cgggccgatc tgatggatgt
     9961 gattgccccg ggcgggctgg gcggcaccta tgccggctcg ccggtggcct gtgcggcggc
    10021 gctggccgtg ctggaagtga tcgaggagga aaagctgctc gatcgtgcca atgcggtggg
    10081 cgcgcgcatc cgcgccgcgg tgcaggctgc tgctgcccgc aatgacacgc tgggcatcgc
    10141 caatctgcgc gggccgggcg cgatgatcgg cttcgacgtg gtggatggcg ccggcaagcc
    10201 cgatgccgcc gctgccaagc aggtcgtcac ccgcgcgctc gacaaggggc tggtggtgct
    10261 cacttgcggc acccatggcg ccggcatccg catcctcgtg ccgctgactg ccagcgacgc
    10321 gctgatcgac gagggcctgg cgatcctgac cgaggcgctg gtgaacgagg aggtggcggc
    10381 ctgatggcaa ccctcgcccc ctcttcgctg gtgctgtccg agcccgatct gctgcgcgat
    10441 gccgcctttg tcggcggcac ctggctggcg ggcgaggaga ccatcgctgt caccaacccg
    10501 gccgatggca gcctggtggg cacggtgccc aatctgggcg cggccgaaac cacgctggcg
    10561 gtggatgccg ccgcccgcgc ccaggcgctg tgggcaaagg aaaccggcaa ggcccgcggc
    10621 caggtgctgg cccgctgggc cgagctgatg gccacccatg ccgacgacct ggcccggctg
    10681 atgaccgccg aacagggcaa gccgctggcc gaggccaagg gcgaagtggc ctatgcggcc
    10741 agcttcctcg aatggtttgc cggcgaggcg cgccgcgtta acggcgagat cctcaccccc
    10801 cacgcggctg accggcgcct gctggtgcgc aaggagccgg tgggcgtggt ggcggcggtc
    10861 accccgtgga acttcccgct ggcgatgatc acccgcaagg cgggcccggc gctggcggcg
    10921 ggctgcgcgg tggtgctcaa gccgtcggag ctgacgccgc tgtcggcgct ggcgctggcc
    10981 cggctggccg agcaggcggg cgtgccggcc ggcgtgttca atgtcgtcac cggcgatgcc
    11041 gcgcccatcg gcgatgtgct gaccggcgac aagcgcatcc gcaagttcac cttcaccggt
    11101 tccacccggg tgggcaagct gctggcggcg cgctgcatgg atacggtgaa gcgggtgagc
    11161 ctggagctgg gcggcaatgc gcccttcatc gtcttcgacg acgccgatct tgatgcggcg
    11221 gtggaagggg cgatggccag caagttccgc aatgccgggc agacctgcgt gtgcgccaac
    11281 cggctgctgg tgcagtcggg catctatgat gcctttgcca gcaagctgtc cgccaaggtg
    11341 cgcgcgctgg tggtgggccc gggcctggcc ggccccagcg accagggccc gctgatcgac
    11401 gggcgcgcgg tggccaaggc cgaggcccat gtgaccgacg cgctggcccg tggcggcagg
    11461 ctgctggtgg gcgggcgcgc caagcccggc cgcggcagct tcttcgagcc gacggtgatt
    11521 gccgaagtgc cgggcgatgc gctgctgtgc cgcgaggaaa cctttgggcc ggtggccggc
    11581 ctcgttcgct tcgagacgga ggaggaggcg gtgcgccttg ccaacgacac cgatgcgggc
    11641 cttgccgctt atgtcttcac gcgcgacatc gaccgcgcct ggcgcgtgcc cgaggcgctg
    11701 caatacggca tggtggggct gaacaccggc cttatcagca ccgaagtggc gccgtttggc
    11761 ggggtcaagg aatcgggcat gggccgcgaa ggctcgcacc acggcatgga tgattatctg
    11821 agcctgaagc tggtgtgcca ggccgtttcc aggggctgag gccaggctgc catatgcggc
    11881 gaaaggggga tgcgcggggg aagccttgcg cctccccctt cgcttttcaa acctgctcca
    11941 tccacccggc gatgccctcg gcgccttcat cgcgcctgag gccggcagcc gcgcccagcc
    12001 ggtctgccgc tgccttcttc tggtccagct tccaggcgcc tgtcagcacc ggcgccacca
    12061 tgcggatgcc aacgatcccc ttcagcagcg cggccagata atccgccggc gcctcggcca
    12121 cggtccaggg gcgggggcgg tggttttcat gggcggcggt cagcggctcc agcagggcca
    12181 gcaggccctc tgcatcctcc accacttcca tctgcccgcg cgcttccacc gcgatatagt
    12241 tccaggtggg caccacgcgg ccggcgtcct gcttgctggg ataccagccg ggcgagatat
    12301 aggcatcgtc gatcaggaag gtggcggcgg cctctgtgcc ggccagcccc cgccattgcg
    12361 gattggcccg cgccagatgg gcgatgaggg tgagctcttc ccccgcgccc gccagcacga
    12421 aaggcaggtg cgagacatgc agctgccccg cgttgaagct ggcgagcgtg ccgaagccgc
    12481 gctggcggat ggcgccgacc agcacctcct ggcgatgttc ctgatagggc ggggggatat
    12541 acatgggcgc gatcctagcg cgccagctgc cgttcagcca tcccaaacgg cggcataaag
    12601 cgcctcgatc tcggcggcgc tggccaggcg cggattgttg gccggcgagc ccgaggccag
    12661 cgcctgttcg gccatcagcg gggtgagcgc gtgccagcgg gccttgtcga tcccgtggcg
    12721 ggcgggcgag ggcacggcca gatcggcatt cagctggcgc agttcctcca ccagcacgcc
    12781 cacggccgcc tgctggccca gccctgccac atccagaccc atggccacag cgcaggcggc
    12841 atagcgttcc agcgcggcag gcgcggaaaa agccgtgatc gccggcagca gcatcgcgtt
    12901 cgacaggcca tgcggcacgt ggaaatgggc gccgatcggc cggctcatgc catgcaccag
    12961 cgcgacagag gcattggaaa aggcaatgcc ggccatggtg gcgccaaaca tcatcgcctc
    13021 acgcgcgggg cggttggccg gatctgccca cacgcggcgc aggttggggg caatggccgc
    13081 catcgccgcc agcgctgcct gatcgctgaa cggatgggcc cggcgcgaga cataggcctc
    13141 gatcgcatgg gtcagcgcgt cgatgccggt atcggcggtg aggcgcgggg gcatgctgag
    13201 ggtcagctcg taatccacca gcgcggccac cggcagatag gcaaggccgg cgcacagcat
    13261 cttctcgtcg cgcgtttcgt cggtgatgat ggtgaagcgg gtggcttccg atccggtgcc
    13321 ggcggtggtg ggaatggcga tgattggcag gcccggggca tcctgcaggt ggggggcctt
    13381 gtagtcctgc gcggtgccgc ccagcgggcc cagcacggca atcgccttgg cgctgtcgat
    13441 cgggctgccg ccgccaaagc ccaccacgca atcatgcggg gctgcggcca ggcagacaag
    13501 gccggcggcg atcgaggcgg tggtcggctc gggcacggta tcggcaaaca cgcgcgaggg
    13561 gatgccggcg gcggcgagcc ccgccaccag cctgtcggcc aggccatgat cggccaggta
    13621 gcggtcggtg acgatcagcg ggtgcgccag gccaaggctg gccagcacgg ccgccagctc
    13681 gcccgaggcg ccggcgccga tgcgcatcag gcggggcagg gcgatggtgt gggtcatggg
    13741 tcgtctcctc cagcggcggg gcgatgatag acgccaatgc gcgcgggtgc cccctgccca
    13801 aatgagcggt ttggcgtttc ccgtggccgg gccccgatcg gccgcttggg caatggccag
    13861 cgccggccgc ttgtggcagc accgggcggg gacaggagaa agggaggcct gcttgcacga
    13921 acatccgctg cgccggcaga ttgtgggcga aatgcatttg cggcgctggc cggcggtggc
    13981 cgcgcctgcc actatcgttc agatgctgcg gcaggtggcg cccgaacagc gcgcggccga
    14041 ggcggcgcat ctggcgcagg tggccgctgc tctgcctgag ggcgcgcgac atgccatggg
    14101 ccaactggcg cccggccttg cctatgtgtg ggaacgccac gccgaggctt cgggcatcac
    14161 gctgtttgcg caagaagggg ccgatccggc ggcggggctg gcatggctgg ccggttttcc
    14221 aggcgccgtg gtgcgcgcta cgcgcatccg ggtggtctct ggcgaagcgc aggtggcagc
    14281 ggcgctggca ggggcgggct ttctggcctc cgatctcgtc agctgcggga tcgggccgga
    14341 agggtgccgg ctgtggtcgg atttccgcat tggcgccgat ggctttggcc agctggtggt
    14401 ggatgtgccc gaagcccccg cgcaagcggc ggcacggcag gggcatgatg tggcccggct
    14461 gctgcaaacg ctgcaggatc tgggcaatta tcgcaatctg gcgctcttgg gcctgccgct
    14521 ggcgcgggcg cactggccgc ggctggatgg ggtggaagag gcgctgcggc agctggcggg
    14581 cgatgtcggc cgggcggatc tgaccgatga tgcgctgctg gagaaggtct gcgcgatcag
    14641 cctggagctg atgtcgatcg gcgcggaaac gcgctatcgg ctgagcgcca cgcgcgccta
    14701 tgcccggctg gtggaagagc ggctgggcca actggcggtg cgccccatcg ccggctatcc
    14761 ctcgctggag gatttcaccc atcgccgctt tttgccggcg gtgcgcacct gttcggcctt
    14821 cgaccggcgg ctgggcgagc tgaccgcgcg cggcgcccag ttcaccgccc tgctgcgcac
    14881 ccgcgtggaa acccgcatcg agaaccagaa cggccagctg ctgcgctcca tggagcagag
    14941 cgcctcgctg caattgcggc tgcaacagct ggtggaaggg ctctcggtgg tggcgctcag
    15001 ctattatgcg ctggcgctgg tgggctatgt gctgaagggc gtggaggaag tggcgccgca
    15061 tttcccggcg gtggcggcag aaggggcgct ggtgccggtg gtggtggcct gcgtgtggct
    15121 ggggctgcac cggatgaagg cgcgcttgct gggcgggcat tgatgggcag cggcaccggg
    15181 cctctccccc acccggccac ccaacgatag taccctaggg gtggccgggt gagggagagg
    15241 gccggtgccg ccctgccgcg ccagcgcggc accaaaaacg cttcacacaa aaaacgggcg
    15301 agggggcgcc ggggttttgc ccctttgccc actcgcccgc gaagaaagcg gcggccgcca
    15361 acgtgatggg aggagcgcgg ccgccgcccg ccggcgtcag aacttgacgc ccagcgttgc
    15421 cacataggtg cgcggcgcgg tgtagttgaa caccgtgagg atagagctgg cgcggatcgc
    15481 ctgctgggtg tcgatgcggt tgaacaggtt cttcacgttg aaggcggcgt aatagcgatc
    15541 gcccttggag ttccaccgga ggccggcatc caccaccacc atcggccggc gcatgctgat
    15601 cggccagggg gtgtcgttgg tgaacagcga ggaggtgtag ttcagctccg aattgaagcg
    15661 tacggcctcg ccgtggtgga gatccagctt gtagtccacc ccgatcgagc cctggaactt
    15721 gggcgcgttg tagagcggga tgccggacca gtcgagcgcg ccggtggtgg tgttgcacac
    15781 ctgcgccgtc ggctggccga ggatctgcgt ggccgagaag ccggcggcgc agaagctctt
    15841 gtaatgggca tcgaggaagc cggcggaacc ggtgatgttg agcccgcggg tgaggcgcgc
    15901 ctccagctcc agctccacgc ccttggtgac ggcagaggcg gcgttgaaca cgttgttgct
    15961 gctttgcccc tggctgttga tgaagttcac cgagcgctgg agatccgaca ggttgttcca
    16021 gaacagggtg atgttggcct tgagccggtg gtggaaccag aagctcttga ggcccgcttc
    16081 ccaggtctcg atgcgttccg gatcataggg gccggcgccg gcaaggctgg tggaaatggc
    16141 cgagaagccg ccgctcttga agcccttggt ccacgaggca tagagcatca tctcgctggt
    16201 cgggcgccag tccaccgaga tcttgggcga gaagttggtc cagctcttct tcgcctcgcc
    16261 atagggcccc acctgcagga acacccccgg cgacaggccg acagcggccg ccaccgtggt
    16321 gggatcgcgc gattgatagg cgaagtagaa gtccttggtc tcgcccattt cgcgcccgcc
    16381 aaagttcagg cgcacattgc gcatcgggcg cacttccagc tcgccgaaca ctgcccagct
    16441 gtcgcgcttc tgcatcacgt tggtttgcga attggccgcg gtgaggggcg cgtattcgga
    16501 ataggtgatg ttgccatagg tgaggtcgtc atgcgcgtaa taggcgccca ccagcagctg
    16561 gaaggtcttg ttcagctcct tgatgccgcg cacttcctgg ctgaaattct tcagctgcgt
    16621 gcggtagtag ttggtgagat aggcgatctt gccgcccacc ggatcggtga acagcgcatc
    16681 gctggaatat tgatagccgg tgatcaggtt gatcgagccg ccctcgatgt tgcgggtgat
    16741 gttgagcgcg acattgtcct ggttgacgaa actgtcctgg ccggggcgga tgttcacatt
    16801 cgtccagtag cgtggataat tgtagaggtt gggcggatcg ccggcgatcg agccgaacag
    16861 ctgggcgggc agcgccgagg aggccatggc cggcgtggtg gaggcgtttt gcgcgggcgt
    16921 gggctggctg cggtcgcgca cgtgctcgta tttgaagaag atgttccagt cgggcgaggg
    16981 ggtgtagagc agcgagatgc gcccggtgat attgtcgtcg ccgcccagcg gtttgccgtt
    17041 ttcctggatc gaatggtaat agccatccga tttggtgacg agcagcgagg cgcgcatcgc
    17101 cagcacatcc tgcacgatcg gcacattcac cgccgcgcgc gtttcccagc gcccgtaatt
    17161 gccgatgcgc ccttccacct ggccgccaaa ttcctgcgtg ggccgcttcg actggatgtt
    17221 gaccgcgccg gcaaaggcgt tgcggccaaa caatgtgccc tgggggccgc gcagcacttc
    17281 caccgattca atatcgaaca tgtcgagcac gttgagggtg gagcgcggct ggtagagccc
    17341 gtcgatatag aaggcggtgg aaggatcgcc ggcctggtcg gagcgggcat agttgacgcc
    17401 gcggatcgcc agcatgcccg cgcgcccctg cgagcccagc gaatcgatgc gcacattggg
    17461 catggagccg cccagatcgg tgatgttctg cggcgccttg gcctcgatct gctccgaggt
    17521 cagcgccgag acggccagcg gcgtgcgctg cagcggctcg gcgcgcttgc gggcggtcac
    17581 cacgatatcg gcgatgctgg tgtcggtgcc cttggcgatg ggggtggtgg gcgcgccttc
    17641 ctcagcctgg gcgttgccgg ccagcgccgc ggcgcaaagg ccgatggcaa gggccagggc
    17701 agagcctgtg gctcgcctcc tctgggcgca ttgatcctgt gtcacgtgat cctctccttt
    17761 gtgttttggg ttatgttctg tgtcaggcag gcacagggca gccgctggcg gcgcgcgcgg
    17821 gcacaggccc ggcgcccgcc cgcaaggggc cccgatcgct ttcgtgaaag gctcggtccg
    17881 ccggtttcag ccggcgcggc cgaggatcag tctctctctc ccgtgggggc ctgggcccct
    17941 tcggtcctgt cggcggcggg cttgtccagg ccggccagaa tgccattgct gatgaagcgg
    18001 atgtaggtgt cgatcacatc ggggctgagc ggcaccacct tgtgcccggc cacgccatag
    18061 cggcgcatga tctgcgagcg cgcgccgatc agcatcaccg ccagctgttc cagctgcgcg
    18121 tggctccagc cttccagcac ggtgccggcg cgtttgcggg ccagcgcgcg cacatagccg
    18181 ttggccatcg catcgatatg ggcggtgtag ctgacgggag aatagagctc ggcctcctgc
    18241 gtcagccggg ccatgaaggg atagcggcag aagaaatcgt ggttggcttc caggccgcgg
    18301 cgttccagtt ccaccaggct gccgcattcg tgcacggcat cgcgcacatg ttccagcatg
    18361 cgcaggctca tctcgcgcag cagttcctcg aacatttcct gctgcgactg gaagtggagg
    18421 tagaaggtgc cgtgggcgat gcgggccctg gccgtgacgc gggcgatggt gcagccggca
    18481 aagccgtatt tgccgatcat ctgcccggct gcttccagca gcttgcgccg ggtggcctcg
    18541 cgccgttcca gctgcccggg cgaggggcgg cggctgcggc gggcaggcgt ggtggccggc
    18601 gcgggcgggg cgggcttgct gcgtgtgggc atcggtcggg atctgtccat caagcgggcg
    18661 cagggggcgc cctgttgccg ccatgccgcg cccatggccg ccatggcaag aggcagggga
    18721 tgcgccgcca tgccgccggc cgcctgccgg ccatggccga tgccgcctac cacgacccta
    18781 cgacagactc gccagaccat gaccatcccc cggatattgc ctgcctttcc gaccggcgct
    18841 gcacctgtgt cggtgcctta atatgaatgg ctaatcattt ttatggtgga tgataacctg
    18901 ccatttctca caggttatga actgcctttg gatgacttga cagtcatatt tagctgcgcg
    18961 acgatccctt gcacaaggcg ggcaggcaag cgggcaacca caacgcccca cctgcgccaa
    19021 ccgggacagg acaggccgag gaccatcatg gcacacagca caacaggcag cggcgcgcct
    19081 gcgccaggca cgcccaaaga tgaaagcggc cttggccttg cctttggcac ctatgaggaa
    19141 gcgctgggct ggattggccg cgcaacagag ccggccaccg gcgaggtggc ggtcaacacg
    19201 ccgatgatcc agctctattg ctcggccgtg caggatgcca accccaccta ttgggacgag
    19261 gcttttgcgc aggaacactg gggctccatg gtggccccgc cgggcatgct gcaaacctgg
    19321 acgatcccca tgcaatggcg cccggaaggg gcgcgcacgg tgtctgtcat gtgcgccaag
    19381 gtgccgctgc cgggcgacaa gccgatcaac gtctccaccg attttgaatt cttcgagccg
    19441 gtgcgcgtcg gcgatcgcct gaccatgacc gaccggctgg aggcgatctc gccggaaaag
    19501 accaccaagg tgggcaccgg ccatttcatc acctcggtca cggaatatcg caaccaggcg
    19561 ggcgtgctgc tggcgcgcag caccaatgtg ctgttccgct atctctcggg agaggcggca
    19621 tgagcgctgc ggcaaggcag atctgctggc aggatgtgga cgaggcaacg ccgctgccgg
    19681 tgctggtgct ggatgtgacc tacaagatgg cgatcctgca tgtggcgggc ggctgggatt
    19741 acatgcccgg ccaccacgat ccggaatttg cccgcaggca gggcgcgccc acggtgttcc
    19801 tcaacacg
//
LOCUS       DASRYE010000170         9854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_96829,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000170 DASRYE010000000
VERSION     DASRYE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96829"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..502
                     /locus_tag="VFF98_08805"
     CDS             74..502
                     /locus_tag="VFF98_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HZU63770.1"
                     /translation="MTLLRLSVAAILAAALPAAPALAHHSYSAFDRSEAAKRHIAGTV
                     RDFALINPHGWLKLVVAEPDGRTASWTFELSSATQLQPLGWSPASIRVGDKVDVTFFP
                     LRFGSYGGQLVAVRLPAGKVLQGTAEPDRGYPKPAGANGK"
     gene            499..1548
                     /locus_tag="VFF98_08810"
     CDS             499..1548
                     /locus_tag="VFF98_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63771.1"
                     /translation="MSRRPLAAGLWALLGTATAALGAEEAPLLWHDNPDWFEADFTSP
                     PATASGSYAAIGPDGARVPVPAATYSATQEGAAYQVIVARLHGSAAADPHVIDRSIAA
                     ARRQPGLALDASTSHSYGTSGAQACGRQFGFAGKDGALLYRTLFYNPRTERLYDIRSE
                     VSAAAQADHGADVVHFQQSFALLADPGAKAETPPPSPASWQDVAAPDKAFTIRFPAAP
                     RIRQEAYRTATGAAVPATVYEARAGAALYRLTAIHLWQTAGDGPGALDSEIARFQHSG
                     TVLEEGPAAIPYGQCGRAIALRTPDGTRREVELFFPSSQHRLYLIEVRHKAESPVDPL
                     DLARFRLSFALAKPE"
     gene            1555..2019
                     /locus_tag="VFF98_08815"
     CDS             1555..2019
                     /locus_tag="VFF98_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63772.1"
                     /translation="MSIDTFASWLDQTPLSEALKNSALIIPAAQSVHILSVAIIVSDA
                     ALALLVALGLAGADWPLARWIGATGRRLGWALGALLATGTLLVAAEPTRELPNAALRL
                     KIALLVPVIAYALWQRRRLARAEPAAAPRIATAVSLALWVVVVGLGRWIAYA"
     gene            2016..2507
                     /locus_tag="VFF98_08820"
     CDS             2016..2507
                     /locus_tag="VFF98_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63773.1"
                     /translation="MTGLLAWLAQTAPAQWLAHNPLAFPLLETVHVLAISLVLGTATI
                     FDIRLLGLGWQGWPAGPLLTGLRKVALAGFAGALASGALLFLSQPESYAQNLPFRAKL
                     ALLALAGLNLALFEGRYGRQQASWQHSASVPAGARVQAGLSLGLWGAVLVAGRLIGFF
                     LQG"
     regulatory      2585..2832
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2952..5078
                     /locus_tag="VFF98_08825"
     CDS             2952..5078
                     /locus_tag="VFF98_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63774.1"
                     /translation="MQRVLFSLCLFAPALCPAPAMAEEGEGQTITVTASRADLLGSAV
                     TASQGTVSQAEVALRPIYRPGELFEAIPGLVVTIHSGEGKAQQYLIRGYNLDHGTDFA
                     SFVDDMPVNRPTNAHGQGYSDLNFLMPQLVAGIDYTKGPYAAAIGDFGSVASAHTRLV
                     NELPAQVAATVGTDGYQNVFAGGTFHLGADKRLLAAVELGHYDGPWQPGQNFRKVNAL
                     LRYSQGSARDGFTLTAMAYASAGRLTTDQPERAIEEGLIGRYGTLDPTDSSKSQRYSL
                     SAHLDRPVGPGQLAVSLYAIRSTMTLWNNFTHYLDDPVYGDQEEQDESRTTLGAAAAY
                     TIATRIAGLDSQTVAGFQLRYDAAFVDRKHTWQRNTILATCYQQSDSGTFAYAAVGGN
                     CTADKVRLLTLSPYVEETLHLARWLRVVGGLRADYTYARDHSLVTGTGGSGGQWLAQP
                     KGSLVLGPWAKTELYVSWGRGFHSNDVRGVFGTVPTQGLSVSGGARTPLLSSTTGMEV
                     GLRSNLLPKLSLQLAAFQQDFGSELVYNPDTGQDEAGAPSRRQGVEISAQYRPFRWLE
                     LNTDLAFSRPRYHTADLAAYGLAEPYIADAPNFIYAAGVLVNGLGRWSGALQWRRLGT
                     HSLNDGYKYPQDGGYSEWNLDVGYALPRGWRLGVSIFNLFNSHDAAADYYYTARLQGE
                     PADGVAGFQTHPLEPRSARFTIGKTF"
     gene            complement(5098..5847)
                     /gene="cobF"
                     /locus_tag="VFF98_08830"
     CDS             complement(5098..5847)
                     /gene="cobF"
                     /locus_tag="VFF98_08830"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HZU63775.1"
                     /translation="MNRLVLIGIGTGHVDHLTLEAVREINAADLVLIPRKGEAKADLA
                     ALRRDICARVLTNPATRIAEFDLPERDPATADYRQRVEDWHDAIAACWAGAIARALPD
                     GGRVALLIWGDPALYDSSLRIAARLEGWAVQVVPGIMSINMLTAAHAIALNEINGPFL
                     VTTGRRLRDEGWPQGVDTLVVMLDGECSFTRLAPAGVEIWWGAYVGMAEQMLIAGPLA
                     EVAPRIVAERAAARARHGWIMDIYLLRRARP"
     gene            complement(5844..7175)
                     /locus_tag="VFF98_08835"
     CDS             complement(5844..7175)
                     /locus_tag="VFF98_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZU63776.1"
                     /translation="MSGALPPGLVISAPASGAGKTMVTLGLLAAFRAAGVRVQPFKNG
                     PDYIDPAFHTAAAGRPSFNLDSWAMDGAMLAGIAGVAAGMDLALAEGSMGLFDGVAAA
                     GAAGNGASAELAARAGWPVVLVVDVSGQAQSAAAVAMGFARFRPDVPLAGVILNKVAS
                     SRHEALVRAGMAEAGIAVLGALPRRTDLALPERHLGLVQAEERTDLPAILAALGAFVG
                     SHVDLAQLRALAAGGGMASAPPARLVPPGQRIALARDAAFSFVYPHLLAGWRQAGAEI
                     LPFSPLADEAPDDAADVCWLPGGYPELHAGRLAAAAHFRTGLRAFAQSRPVHGECGGY
                     MAMGAVLVDAGGAAHPMAGLLGLETSFARRRMHLGYRLARLAGPMPGQPAGALLRGHE
                     FHYATITAQPDAPLAEVADANGDAVAETGSRRGLATGSFFHLIAPAGGGAA"
     gene            complement(7172..7957)
                     /gene="cobM"
                     /locus_tag="VFF98_08840"
     CDS             complement(7172..7957)
                     /gene="cobM"
                     /locus_tag="VFF98_08840"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZU63777.1"
                     /translation="MTVHFIGAGPGAADLLTLRGARLIAASPVCLYAGSLVPEGVLAH
                     CPPGARLVNTAPLSLDEIIAEIARAHAAGQDVARLHSGDLSVWSAMGEQLRRLRALGI
                     PFDVTPGVPSFAAAAAALEAELTLPGVAQSLVLTRTSGRASAMPPGETLAAFAATGAT
                     LAIHLSIHALDRVVAELTPHYGPDCPVAVVWRASWPDERICRATLATLDAALGAELER
                     TALILVGRAIGAGDFAESRLYAADYDRRYRPQGTAPRFPETAP"
     gene            complement(7954..8343)
                     /locus_tag="VFF98_08845"
     CDS             complement(7954..8343)
                     /locus_tag="VFF98_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HZU63778.1"
                     /translation="MSTPKPIAGLGWRGAATPAALGEALARAEALAGGAPVAALAALA
                     SMAGAPALRALAGARGLPVLPIAAEALAGVATPTCSARMIARFGTGSLAEALALVAAG
                     PGARLIAPRHISTDGMATAAIAQRSLP"
     gene            complement(8340..9497)
                     /gene="cbiE"
                     /locus_tag="VFF98_08850"
     CDS             complement(8340..9497)
                     /gene="cbiE"
                     /locus_tag="VFF98_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZU63779.1"
                     /translation="MGEDRPMALPPASLAALAAAAHVFGAPRHLELAGLADDPRAKAW
                     PVPFSVAPVLALRGAQVAVLASGDPFWFGAGGSLARHLAAGEWAAYPAPSTFALAAAR
                     LGWRIEEVACMGLHAAPFEQLAPLMDAGARAICLLRDGTAAGALAAWLAARGFGASQC
                     HVLERLGGPQERCRTTRADAFALADVAAPVAMAVAFAGARGIARSSGLPDHLFSHDGQ
                     ITKRPVRALTLSALGPRAGELLWDLGAGSGSVGIEWCLAGGRAVAVERHEGRLAHIRA
                     NAQSFGLAHRLQVVAGDTAAVLADLPAPDAVFIGGGASAALIDAAWARLGAGGRLVAN
                     GVTLETEAVLAAAQARHGGELLRVELAHARPLGRFRGWEAVRPVVQWSAWR"
     gene            9502..>9854
                     /locus_tag="VFF98_08855"
     CDS             9502..>9854
                     /locus_tag="VFF98_08855"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HZU63780.1"
                     /translation="MIDSHASATPRLLLLGGTSEASQLARALAAAGVDTVFSYAGRTA
                     APLAQPLPTRIGGFGGADGLAAWLRAEGITHVVDATHPFAATISRNALAACAATGVPL
                     AAFERPAWQAGPGDR"
BASE COUNT         1484 a         3767 c         3263 g         1340 t
ORIGIN      
        1 ccaccagcca gagcccgtgg ccgcggcctg accgcgcccg cccgcccccg caaacagccg
       61 aaagctgcct ctgatgactc tcttgcgcct gtctgttgcc gccatccttg ctgcggccct
      121 gcccgccgcg cccgcgctgg cccaccattc ctattccgcc ttcgaccgat cggaagcggc
      181 caagcgccac atcgccggca cggtgcgcga tttcgcgctg atcaacccgc atggctggct
      241 gaagctggtg gtggccgagc ccgatggccg caccgccagc tggaccttcg aactctccag
      301 cgccacgcag ctgcaaccgc tgggctggtc gcccgccagc atcagggtgg gcgacaaagt
      361 ggacgtcacc ttcttcccct tgcgcttcgg ctcctatggc gggcagctgg tggcggtgcg
      421 gctgccggcg ggcaaggtgc tgcagggcac cgccgagcct gatcgcggct atcccaagcc
      481 ggccggggcc aacggcaaat gagccggcgc ccgctcgccg ccggcctctg ggccctgctt
      541 ggcaccgcca ccgccgcctt gggcgccgag gaagcgccgc tcctctggca cgacaatccg
      601 gactggttcg aggccgattt caccagcccg ccggccaccg ccagtggcag ctatgccgcc
      661 atcggccccg atggcgcccg cgtgccggtg cccgccgcca cctacagcgc cacgcaggaa
      721 ggcgctgcct atcaggtgat cgtcgcccgg ctgcatggct cggccgcggc cgatccgcat
      781 gtgatcgacc gcagcattgc cgccgcccgc cgccagcccg gcctcgccct cgacgcctcg
      841 accagccaca gctatggcac atcgggcgcg caggcctgcg ggcgccagtt cggctttgcc
      901 ggcaaggatg gcgcgctgct ctaccgcacg ctgttctaca acccgcgcac cgagcggctc
      961 tacgatatcc gctccgaggt cagcgccgcc gcccaggccg atcatggcgc cgatgtcgtg
     1021 catttccagc aaagcttcgc cctgctggca gatcctggcg ccaaggccga aacaccgccc
     1081 ccctcgcccg ccagctggca ggatgtggcc gcgcccgaca aggccttcac catccgcttt
     1141 cccgccgccc cgcggatcag gcaggaggcc tatcgcaccg ccaccggcgc ggctgtgccc
     1201 gccaccgtct atgaggcgcg ggccggcgcc gcgctctacc ggctcaccgc catccacctg
     1261 tggcagacag cgggcgatgg ccccggcgcg ctcgacagcg agatcgcccg cttccagcac
     1321 agcggcacgg tgctggagga agggccggcg gcgatcccct atggccagtg cggccgcgcc
     1381 attgccctgc gcacgccaga cggcacccgg cgcgaggtgg agctgttctt ccccagctcg
     1441 cagcaccggc tctatctgat cgaggtgcgc cacaaggccg aaagccccgt cgatccgctc
     1501 gatctggcgc gctttcgcct ctccttcgcg ctggccaagc cggaatagcc gcccatgtcg
     1561 atcgacacat tcgccagctg gctcgaccag acaccgctca gcgaggcgct gaagaacagc
     1621 gcgctgatca ttcccgccgc ccagtcggtg catatcctca gcgtggcgat catcgtctcg
     1681 gatgcggcgc tggcgctgct ggtggcgctg gggctggccg gcgccgactg gccgctggcc
     1741 cgctggatcg gcgccaccgg ccgccggctg ggctgggcgc tgggcgcgct gctggccacc
     1801 ggcacgctgc tggtggcagc agagcccacg cgtgagctgc ccaatgccgc cttgcgcctc
     1861 aagatcgccc tgctggtgcc ggttatcgcc tatgcgctgt ggcagcgccg ccgcctggcc
     1921 cgggcagaac cggcggccgc gccgcgcatc gccacggcgg tgtcgctggc gctgtgggtg
     1981 gtggtggtgg gccttggccg ctggatcgcc tacgcatgac ggggctgctt gcatggctgg
     2041 cgcaaaccgc gcccgcgcaa tggctggcac acaacccgct ggccttcccg ctgctggaaa
     2101 cggtgcatgt gctggccatc tcgctggtgc tgggcacggc caccatcttc gatatccgcc
     2161 tgctggggct gggctggcag ggctggcccg ccggcccgct gctcaccggc ctgcgcaagg
     2221 tggcgctggc cggctttgcc ggcgcgctgg ccagcggcgc cctgctcttc ctctcgcagc
     2281 cggaaagcta tgcccagaac ctgcccttcc gcgccaagct ggcgctgctg gcgctggccg
     2341 gcctgaacct ggcgctgttc gaggggcgct atggccgcca gcaggccagc tggcagcaca
     2401 gcgccagcgt gcccgccggc gccagggtgc aggcggggct gtcgctgggg ctgtgggggg
     2461 cggtgctggt ggcgggccgg ctgatcggct tcttcctgca gggctgaaac cggcctctgc
     2521 gataagccgg cttggcagcc tgcagcaatt cgtctagcct tttcatcggt gcggcccccg
     2581 gccgttacca ataacacgcg ctggtgcccg caagggcttg aaagggaaca cgggtgcggc
     2641 cccgccggaa ggcaggccaa atcccgggct gtccctgcaa ctgtaagcga cgagcgcgct
     2701 gcatgtgatg ccccgccaag gcgccggggc gcggccactg gaccagacgc agaagcatgc
     2761 ggggtccggg aaggctttgc atcgcgtgat gacccgcaag ccaggagacc tgcccgcgcg
     2821 tggtcgctct tgccctgatc caggggatga tcaaggcacg gtcctttccg tccgagcggc
     2881 aaaaccgtca acccgcaggc tggctcgcgc cggccctgcg gtcctgtgtg tcttgctaac
     2941 ggaacctgcc gatgcaacgc gttcttttct ccttatgcct gtttgccccc gccctgtgcc
     3001 cagccccggc catggccgag gagggcgaag gccagaccat caccgtcacc gcctcacgtg
     3061 cggatcttct gggcagcgcc gtcacggcca gccagggcac ggtgagccag gccgaagtgg
     3121 cgctgcggcc catctatcgg cccggcgagc tgttcgaggc gatcccgggc cttgtcgtca
     3181 ccatccattc gggcgagggc aaggcgcagc aatatctcat ccgcggctac aacctcgatc
     3241 atggcaccga ttttgccagc tttgtcgacg acatgccggt gaaccggccg accaacgccc
     3301 atggccaggg ctattccgat ctcaacttcc tgatgcccca gctggtggcc ggcatagact
     3361 ataccaaggg cccctatgcg gccgccatcg gcgatttcgg ctcggttgcc tcggcgcaca
     3421 cccggctggt gaacgagctg cccgcgcaag tggcggccac cgttggcacc gatggctatc
     3481 agaatgtctt tgccggcggc acctttcacc tgggcgccga caagcgcctg ctggccgctg
     3541 tggaactggg ccattacgac ggcccctggc agcccgggca gaacttccgc aaggtgaacg
     3601 ccctcctgcg ctacagccag ggcagcgcgc gcgatggctt caccctcacc gccatggcct
     3661 atgccagcgc cggccgcctc accaccgacc agcccgagcg cgccatcgag gaagggctga
     3721 tcggccgcta tggcacgctc gatcccaccg attccagcaa gtcgcagcgc tacagcctct
     3781 cggcccatct cgacaggccg gtgggccccg gccagctggc cgtcagcctc tatgccatcc
     3841 gctccaccat gacgctgtgg aacaatttca cccactatct cgacgacccc gtctatggcg
     3901 atcaggagga gcaggacgaa agccgcacca ccctgggcgc ggccgccgcc tacaccatcg
     3961 ccacccgcat cgccgggctc gacagccaga cggtggccgg cttccagctg cgctatgatg
     4021 ccgcctttgt cgatcgcaag cacacctggc agcgcaacac catcctcgcc acctgctacc
     4081 agcaaagcga cagcggcact ttcgcctatg ccgcggtggg cggcaattgc acggcagaca
     4141 aggtgcgcct gctcaccctg tctccctatg tggaagaaac gctgcatctg gcccgctggc
     4201 tgcgcgtggt ggggggcctt cgcgccgatt atacctacgc gcgcgatcac agcctggtga
     4261 ccggcaccgg cggcagcggc gggcaatggc tggcccagcc caagggcagc ctggtgctgg
     4321 gcccctgggc caaaacggag ctgtatgtca gctggggccg gggcttccat tccaacgatg
     4381 tgcgcggcgt gtttggcacg gtgccaacgc aggggctgtc ggtatcgggc ggggcgcgca
     4441 cgccgctgct gtcgtccacc accggcatgg aagtggggct gcgcagcaat ctgctgccca
     4501 agctcagcct gcagctggct gccttccagc aggatttcgg ctccgaactg gtctataacc
     4561 ccgataccgg gcaggatgag gccggcgccc ccagccggcg ccagggcgtg gaaatatcgg
     4621 cccaataccg ccccttccgc tggctggagc tgaacaccga cctcgccttc tcgcgcccgc
     4681 gctatcacac ggccgacctc gccgcctatg gcctggccga gccctatatc gccgatgcgc
     4741 ccaatttcat ctatgccgcc ggcgtgctgg tgaacgggct ggggcgctgg tcgggcgcgc
     4801 tgcaatggcg gcggctgggc acccattcgc tcaacgatgg ctacaaatat ccgcaggatg
     4861 gcggctattc ggaatggaac ctcgatgtcg gctatgccct gccgcgcggc tggaggctgg
     4921 gcgtcagcat cttcaacctg ttcaacagcc acgatgccgc cgccgactat tactacaccg
     4981 cccgcctgca gggcgaaccg gccgacggcg tggccggctt ccagacccac ccgctggaac
     5041 cccgctccgc ccgcttcacc atcggcaaaa cattctgacc atgatgcgcg cctgccctca
     5101 ggggcgggcg cgccgcagca ggtagatgtc catgatccag ccatggcggg cgcgcgctgc
     5161 cgcccgctcg gccacgatgc gcggcgccac ttcggccagc ggcccggcga tcagcatctg
     5221 ctcggccatg cccacatagg cgccccacca gatctccacg cccgcaggcg ccagccgcgt
     5281 gaacgagcat tcgccatcca gcatcaccac cagcgtgtcc acgccctgtg gccaaccctc
     5341 gtcgcgcagg cgccgcccgg tggtgaccag gaacggcccg ttgatctcgt tgagggcgat
     5401 cgcatgggcc gccgtcagca tgttgatcga catgatcccc ggcaccacct gcaccgccca
     5461 gccctccagc cgggcggcaa tgcgcaggct gctgtcatac agcgccggat cgccccagat
     5521 cagcagcgcc acccgcccgc catcgggcaa cgcccgggcg atcgccccgg cccagcaggc
     5581 ggcaatggca tcgtgccagt cttccacccg ctggcgataa tcggctgtgg ccgggtcgcg
     5641 ctcgggcagg tcgaattcgg cgatgcgcgt ggcagggttg gtcagcacgc gggcgcagat
     5701 gtcgcggcgc aatgcggcca gatccgcctt cgcctcgccc ttgcgcggga tcagcaccag
     5761 atcggcggcg ttgatctcgc gcacggcttc cagcgtcaga tgatcgacat ggccggtgcc
     5821 gatgccgata agcaccagcc ggttcatgcc gcccccccgc cggccggcgc gatgaggtgg
     5881 aagaacgagc ccgtggccag cccccggcgc gatccggtct cggccaccgc atcgccattg
     5941 gcatcggcca cttcggccag cggcgcatcg ggctgggcag tgatggtggc atagtggaat
     6001 tcatggcccc gcagcagggc gccggcgggc tggcccggca tgggcccggc cagccgcgcc
     6061 agccgatagc ccagatgcat gcgccgccgg gcaaaggagg tttccagccc cagcagcccg
     6121 gccatcggat gcgcggcccc gccggcatcc accagcaccg cgcccatcgc catatagccg
     6181 ccgcattcgc catgcaccgg gcggctttgg gcaaaagcgc gcagccctgt gcgaaaatgc
     6241 gccgccgccg ccagccggcc ggcatgcagt tccggatagc cgccgggcag ccagcagaca
     6301 tcggcggcat catcaggcgc ctcatcggcc agcggcgaga agggcaggat ctcggccccc
     6361 gcctgccgcc agccggccag cagatgggga tagacgaaag aaaaggccgc atcgcgcgcc
     6421 agggcaatgc gctggccggg cggcaccagc cgggccggcg gtgccgatgc catcccgccg
     6481 cccgccgcca gcgcccgcag ctgcgccaga tcaacatggc tgccgacaaa ggcgcccagc
     6541 gctgccagaa tggcgggcag atccgtgcgt tcctcggcct gcaccaggcc cagatggcgc
     6601 tctggcaggg ccagatccgt gcggcgcggc agggcgccca gcacggcgat gccggcctct
     6661 gccatgccgg cgcgcaccag cgcctcgtgc cggctgctgg ccaccttgtt gaggatcacc
     6721 ccggccagcg gcacatcggg gcggaagcgg gcaaagccca tggccaccgc cgctgccgat
     6781 tgcgcctggc ccgacacatc gaccaccagc accaccggcc agccggccct ggccgccagt
     6841 tcagcgctgg cgccattgcc ggcagcgccc gccgccgcca cgccatcgaa cagccccatg
     6901 gagccttcgg ccagcgccag atccatgccc gcggcaacgc cggcaatgcc ggccagcatc
     6961 gccccgtcca tcgcccagct gtcgaggttg aacgaggggc ggccggcagc ggcggtgtgg
     7021 aaggccgggt cgatataatc cggcccgttc ttgaacggct gcacccgcac gccggcggcg
     7081 cgaaaggcgg ccagcaggcc cagcgtcacc atcgtcttgc cggcgcccga ggccggcgcc
     7141 gagatcacca gccccggcgg cagggcgccg ctcatggcgc ggtctcgggg aagcgcgggg
     7201 cggtgccctg cgggcgatag cgccggtcgt aatcggcggc atagagccgg ctttcggcaa
     7261 aatcacccgc gccgatcgcc cggccgacaa ggatcagcgc ggtgcgctcc agctcggccc
     7321 ccagcgccgc atccagcgtg gccagcgtgg cgcggcagat ccgctcatcc ggccagctgg
     7381 cgcgccacac caccgccacc gggcaatcgg gcccgtaatg gggggtcagc tcggccacca
     7441 cgcgatccag cgcatggatc gacaggtgga tcgccagcgt ggcgccggtg gcggcaaagg
     7501 cggccagcgt ttcgcccggc ggcatggcgc tggcccggcc cgatgtgcga gtgagcacaa
     7561 gcgattgcgc cacgcccggc agggtcagtt ccgcctccag cgcggcggcg gcggcggcaa
     7621 aggagggcac gccgggcgta acatcaaacg gaatgcccag cgcccgcagc cggcgcaatt
     7681 gctcgcccat cgccgaccat accgacagat cgcccgaatg cagccgcgcc acatcctggc
     7741 cggcggcatg ggcacgggcg atctcggcga tgatctcgtc gagcgagagg ggcgccgtgt
     7801 taaccagccg cgcgcccggc gggcaatgcg ccagcacccc ttcgggcacc agcgatccgg
     7861 catagaggca caccgggctg gccgcgatca gccgcgcgcc gcgcagggtc agcagatcgg
     7921 cagcgccggg gccggcgccg atgaaatgca cggtcatggc aagctccttt gggcgatggc
     7981 ggccgtggcc atgccatctg tggatatatg gcgcggcgcg atcagccgcg caccgggccc
     8041 ggcggccacc agcgccagcg cctcggccag cgatccggtg ccaaagcggg cgatcatccg
     8101 cgcgctgcaa gtgggcgtgg ccacgccggc cagcgcctcg gccgcgatcg gcagcaccgg
     8161 cagcccccgc gcgccggcca gcgcccgcag cgccggcgcc cccgccatgg aagccagcgc
     8221 cgccagcgcc gccaccggcg cgccgccggc cagcgcctcg gcccgggcca gggcttcgcc
     8281 aagcgccgcc ggggtggccg cgccccgcca gcccagcccg gcgatcggtt tgggcgtgct
     8341 catcgccagg cgctccactg caccaccggg cgcaccgctt cccagccgcg aaagcggccc
     8401 agcgggcgcg catgggccag ctccacgcgc aacagctcgc cgccatggcg cgcctgcgcg
     8461 gcggccagca cggcttctgt ctccagcgtc acaccattgg ccaccagccg cccgcccgcg
     8521 cccaaccgcg cccatgccgc atcgatgagc gccgcgctgg ccccgccgcc gatgaacacc
     8581 gcatcgggcg cgggcagatc ggccagcacc gccgccgtat cgcccgccac cacctgcagg
     8641 cggtgcgcca ggccaaagct ttgggcattg gcgcggatat gggccagccg cccttcatgc
     8701 cgctccaccg ccacggcgcg gccgcccgcc aggcaccatt cgatccccac cgagcccgag
     8761 cccgcgccca gatcccacag cagctcgccc gcccgcggcc ccagcgccga aagggtgagc
     8821 gcgcgcaccg gccgcttggt gatctggcca tcgtggctga agaggtgatc gggcaggcca
     8881 ctgctgcggg cgatgccgcg cgcgccggca aaggccaccg ccatcgccac cggggctgcc
     8941 acatcggcca gggcaaaggc atcggcgcgc gtggtgcggc agcgctcctg cgggccgccc
     9001 agccgttcca gcacatggca ctggctggcg ccaaagccgc gcgcggccag ccatgccgcc
     9061 agcgcgccgg ccgccgtccc atcgcgcagc aggcagatcg cccgggcgcc tgcatccatc
     9121 agcggcgcca gctgctcgaa gggggcggca tgcaggccca tgcaggccac ttcctcgatc
     9181 cgccagccca gccttgccgc cgccagcgca aaggtggaag gcgccggata ggccgcccat
     9241 tccccggccg ccagatgccg cgccagactg ccgccagcgc caaaccagaa cggatcgccc
     9301 gaggccagca ccgccacctg cgccccccgc agcgccagca ccggcgccac cgaaaacggc
     9361 accggccagg ctttggctcg cggatcatcg gccagccctg ccagttccag atggcggggc
     9421 gcgccaaaca catgcgccgc tgcggccagc gcggcaaggc ttgccggcgg cagcgccatc
     9481 ggcctatcct cgcccatgcc gatgatcgac agccatgcct cagccacgcc gcgcctcctc
     9541 ctcctgggcg gcaccagcga agccagccag ctggcccggg cgctggccgc agccggggtg
     9601 gatacggtgt tctcctatgc cgggcgcacg gccgcgcccc tggcccagcc gctgcccacg
     9661 cgcatcggcg gctttggcgg agccgacggg ctggccgcct ggctgcgggc ggaagggatc
     9721 acccatgtcg tggatgcaac gcaccccttt gccgccacca tcagccgcaa cgcgctggcc
     9781 gcttgcgctg ccaccggcgt gccgctggcc gccttcgagc ggccggcatg gcaggctggc
     9841 cccggcgatc gctg
//
LOCUS       DASRYE010000171        22653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_80989,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000171 DASRYE010000000
VERSION     DASRYE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 22653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22653
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80989"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="VFF98_08860"
     CDS             complement(<1..2353)
                     /locus_tag="VFF98_08860"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZU63781.1"
                     /translation="MPLLRLSSARRRLAGAALLLSATALAGQAMAQHIPEGEAFAGKI
                     AQSRAQSVPAFPRPAEAPKGAPNVLLVLIDDAGFAATTTFGGGARTPNLDRYAAEGLR
                     YNRFHVTGICSPTRAALLTGRNHHQVGFGNLADIAAGFPAYNSIWKQETGSIARILQL
                     NGYSTAAFGKWHNTPRWEVTPAGPFNHWPTGLGFDHFYGFMSGDDNQWEPHLYNDTTA
                     TPLPATPEQGYHFTTDIVNRAIGWVDDHNAAAAAKPYFLYFATGAVHMPHHVPQEWIA
                     RNKGRFDAGWDAYRTAAFERQKRLGIIPRDTKLTPRPDGLPAWDSLTAAQKQLYAHEM
                     EVFSAYLEQTDAEVGRLIDTLRSRPGGDNLLVLYIVGDNGGSAEGDIEGAIANEQAKA
                     GGAPADFATIAAHADQLGGPEYANHYAAGWAWATSTPFQWMKQVASHFGATRDPLIVD
                     WPGHTRDSGRIRAQFAHVNDIAPTILEAAGIHAPETIDGVRQLPFEGRSLLASFTNPD
                     APETHTEQYFEIFGNRAIYKDGWVAAAKRSYQPWDLLKDVGRIYNDNTAQDRWELYHI
                     TEDFSEATDLARANPAKLAELQAEFLREGGRNGVFPLIPLPFGAPLAVSPKKVSFSFQ
                     PDISGLPVAAQPVLVGRAHHLEIDLKGDAAKGEGVLIAHGGRLGGYVFYVKGGRLVFE
                     NNAYNLRHEVIAASEPLPAGAATVGYDFVPAPGGEAGALPLLSKVGGGTIRLLVNGKV
                     VAEGAVSRFPTHAAAFDETFDIGRDRNSKVSREATAQQPYSGLL"
     gene            complement(2504..3751)
                     /locus_tag="VFF98_08865"
     CDS             complement(2504..3751)
                     /locus_tag="VFF98_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HZU63782.1"
                     /translation="MAIASRLGLQRSAAAPFHLMAKPSGPQCNLDCTYCFYLEKEALF
                     AERKQARRMSAEALEAYVRQTIAATPRQAPVQFTWQGGEPALLGIDFYRQAVALQKRH
                     GAGRAIENSFQTNGTLIDAEWARFFAEQGFLVGLSLDGPASVHDRYRRYANGKPSHAQ
                     VMAALRHLQAAGVAYNVLACVDAHSSQAPLEVYRFLRDEAQVEFVQFTPVVERVAGAQ
                     AAAQGLALNGPGAAADAAVAPFSVAPAAWGQFLNAVFDEWRQHDVGRVFVMNFEWTLA
                     AHLGAPGVVCHHQPVCGRALVAEHNGDVYACDHFVYPEYKLGNLASDSLVAMVDGARA
                     QAFGKAKYETLPGQCRACPHLGACWGGCPKHRFATTAAGEPGLNYLCEGFTLYLSHTA
                     PVIARLGAAIRAGRDPASAAAAG"
     gene            4148..5107
                     /locus_tag="VFF98_08870"
     CDS             4148..5107
                     /locus_tag="VFF98_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZU63783.1"
                     /translation="MATVVASYPNAKDPASPLDIRPVAGRIGAVIHGIRLGADLDDAT
                     IAAIEAALIRHKVVFFRDQAHLTDAEHEAFTARFGDPVAHPTVPVVEGSRYLLELDSK
                     EGYAASSWHTDVTFVDAYPKASILRALTIPEAGGDTLWANGETAYDGLPEALRQLVDN
                     LWAVHSNDYDYAAVLNHRTDEDSEVLNERKKFQKSVFASTIYETEHPVVRVHPVSGQR
                     SLLLGHFVRRFVGLNEVDSARILNVLQDHITRPENTVRWRWQPGDVAFWDNRSTQHRA
                     IADFGLQRRTLRRATVHGEVPVGIDGRQSRTTRQEKAGLTPAA"
     gene            complement(5157..7037)
                     /locus_tag="VFF98_08875"
     CDS             complement(5157..7037)
                     /locus_tag="VFF98_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZU63784.1"
                     /translation="MPGTPGAALAQDTHDGAWMHSSAPPADAYRDPGARLDAAVQAKG
                     KEVYQTICSGCHEQGLNRAPQKYIIAQLTPEAVYHALTAGVMQPMAASLDDGQKRAVA
                     QYVTHQALGTHTAPPPRMCAPKANWFDDRAPPAFAGWGLDLANTHREADELAGIDRAN
                     VGKLHLKWAIGFPGALRARSQPAIAGGAIFVGSHDGSVYALDAETGCARWIFHARGEV
                     RTAPVVAPWTPGGHGAHPLVYFSDLVGNVYALDATTGTRVWSLHADDHPSTTLTATPA
                     LYRDTLYIPVSSLEEGAAASPGYSCCTFRGSLLAVDARTGATRWRTYFTDKPQPRGLN
                     ADGVQVFGPSGVAIWNTPAIDPARGLLYLDTGDNYSDPVTPMSDAIVAMDLGTGAIRW
                     SYQARAGDVWNGSCEDRDTANCPAQKGPDYDFGAGVVLARGVGGHDYLLAGQKSGTAY
                     ALDPDTGRLVWQAQIGRGGVVGGIHFGMATSNGLLYVPISDAPDGRTYAEPPHPGLFA
                     LDVATGARVWQAPAPAGVCGDRPLCHPGYSAAITATSQLVLAGSNDGHLRIFDAASGA
                     VLWDADTTGPVATVSGQPAHGGSMSGGAAPIAWHGLLLVETGYGFAGKMPGNAMLVYG
                     VR"
     gene            complement(7241..7570)
                     /locus_tag="VFF98_08880"
     CDS             complement(7241..7570)
                     /locus_tag="VFF98_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HZU63785.1"
                     /translation="MPTIRDFHAHIYFDPDQAGVAGALGQAIIAAFPETRMGRVHLQP
                     VGPHPRGSCQITVPVASFAPVTMWLLGHRGALTIFMHANTGDDLADHTAHVLWLGPSE
                     ALDLSRF"
     gene            complement(7675..9459)
                     /locus_tag="VFF98_08885"
     CDS             complement(7675..9459)
                     /locus_tag="VFF98_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZU63786.1"
                     /translation="MTFVHPPASPARLRDHSADGRMLLLALAAVPVGAGGAAGAWALL
                     GLIALATNLFWHARLATLPAAMMAPLPLLVALPVLGSVIVGLMARFGSEKIRGHGIPE
                     AIEAILYGESRLSLKVAILKPLSSAISIGSGGPFGAEGPIIMTGGAIGSLFAQGFHLS
                     AAERKTLLVAGAAAGMTAIFGTPLAAVLLAVEVLLFEWKPRSFVPVVIAVLVASALRP
                     GWIGAGPMFPAALGVPAGAWPLAAALGLGALAGLLAGLLSALLYRIEDGFHRLPVHWM
                     WWPALGGLAVGLGALVEPRVLGAGYGNIQALLDGGMTARATALLLVMKAAVWLVALGS
                     GTSGGVLAPLLMLGGALGALVGLLLPGGAGVWAMLGMAGIMSGAMRAPLTGALFAAEL
                     TGHLEALPLTLAAAAGAHAVSVLLMRRSILTEKIARRGRHILQEYTVDPLDLIQAGAI
                     MTPDPQTLPGTMPMAEAAAFLAGPAGHRSYPVVDAAGRLLGLVSRGDALALGPGGAEP
                     GATLADRLSDASQPCATPETPAAAIADLILESGIGRIPVVAPGSGQVIGIVTRHDLLK
                     VRHRVRAEEHRPPRPGRSSHRAAHLAAH"
     gene            9532..9945
                     /locus_tag="VFF98_08890"
     CDS             9532..9945
                     /locus_tag="VFF98_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU63787.1"
                     /translation="MPDSEADLTDADYAVLAAFRFALRSFLAFSEGKAAACGLTPQQH
                     QALLAIRAAPEARATIGYVAERLILKPHSASGLVERLEGLGLLRRAPSPTDRRQSVLS
                     LTPEAETVLRDLSATHREEIRRLRPLLTGLLAHIG"
     gene            complement(9995..10456)
                     /locus_tag="VFF98_08895"
     CDS             complement(9995..10456)
                     /locus_tag="VFF98_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63788.1"
                     /translation="MHSLEQRIRELEDRAELERVLLQYYTAVDTLADMDGLLACFTPD
                     ALFDVADLGLEVYRGHGAIRAFFQGVFADTRYHCHHVTNFHINHLAGDEAKARGYVIG
                     KAEGRGGASVFVHCCYDITYVRTAQGWKISVFDEDSLVPMGDEVSVLHGRR"
     gene            complement(10498..10839)
                     /locus_tag="VFF98_08900"
     CDS             complement(10498..10839)
                     /locus_tag="VFF98_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63789.1"
                     /translation="MGKFSFVCLVNAADGQDAAFSAWHTQEHLPAMVRHGGFVRAKRM
                     RLVQPLDTQQDSYRYLILYEGEVPDTAQALDALNRAIAAGQITFSDTLAPQSWAAVYE
                     EIAGACYPAPF"
     gene            complement(10865..12208)
                     /locus_tag="VFF98_08905"
     CDS             complement(10865..12208)
                     /locus_tag="VFF98_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU63790.1"
                     /translation="MSTDAAALRPAYPLAPPGPPVRLRHDPITGERYYSPAFARAEWE
                     HMWTRIWHIGGLMAELEEPGDYVVHDFLRESVIIVRQPDGAIKAFHNACRHRGNQLVQ
                     GQAGSVPHGFACPYHGWRYGLDGLVRQAQDPENFARGNPCGKVGLKEIPCASWGGFAW
                     YSMDPAAPALADYLAPIPALLASRGIEQMTRVLWTTTRVNTNWKFAPDNFNESYHLPT
                     VHPQMQQIVDEDYRNTVFEMHPAGHNRMIEQGQPSLRATHPNEVEPAWEDMLRAWDLD
                     PADFAGRSRDGRLALQRQKRRLGPARGHAHFASLHDDELTDYFHHTLFPNVTLTATCD
                     GLHLYRTEPDPRDPEWCTFDYWYFVPPVAGRDEVQTIFGARPFAPAAHETFTYGQGAG
                     SAHPLGNFLDQDLAVAVSQQNGFHSRGYQDAYLSDQEDRIRRFHEVLNDYLEGRR"
     gene            12383..13087
                     /locus_tag="VFF98_08910"
     CDS             12383..13087
                     /locus_tag="VFF98_08910"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63791.1"
                     /translation="MAPPIRKTRLDPEGVRQRILDAAESLFAERGYAATPLRDVAAIA
                     GVPVSLITHHFGTKEDLFKQVIARRIHDHVASMRAQLETARQQAAGAPIGAEAFVRAY
                     IGPMISLSVHGGPGWKNYARLLGLAMNSRQYEEFLKPMIEVYDPVNAEFVGELRRIYP
                     DAALRSLHWGMYFLNSTILHVLVEAGMVDRQSQGLCRSAELEAILEEMVPFFAAAFAQ
                     RLGGKATPLPEANLPI"
     gene            13249..14415
                     /gene="hmpA"
                     /locus_tag="VFF98_08915"
     CDS             13249..14415
                     /gene="hmpA"
                     /locus_tag="VFF98_08915"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HZU63792.1"
                     /translation="MLTAEQTAIVKATVPLLETGGEALTTHFYRMMLAEHPEVRPLFN
                     QAHQASGTQPRALANAVLQYARHIDDLGALGALPAQIIQKHVALQILPEHYPIVGGCL
                     LRAIREVLGAEIATDAVIAAWGAAYGQLADILIGAEGAEYDRLAAAPGGWRGTRRFRI
                     AAKVPESAEITSFHLVPADGGPVVAHQPGQYLGLRLTIDGQEVRRNYSLSQAANGRDL
                     RISVKREPGGVVSTHLHDVLAEGDELDVFPPAGAFVLRPGSGPVALISGGVGITPTLA
                     MAESALAQGRDLVFIHYARNPQVQAFGATLAQWARQHPGFTAHVVLEEGAATGAPAGR
                     PSLAQLSAWVPVEGDAYVLGPKPFMAFVNRALAELGVAEGRRHFEFFGPAEALA"
     gene            14447..15151
                     /locus_tag="VFF98_08920"
     CDS             14447..15151
                     /locus_tag="VFF98_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HZU63793.1"
                     /translation="MLRFPALAAFLPHSPPAGLRETARAAAGAGLGIALTALVSTLLA
                     GGHGALPLLIAPMGASAVLLFGVPASPLAQPWSIIGGNLVAGIIGVAAAHTIAAPGLA
                     AAVAVALALAAMTVLRCVHPPSGAVALTAVLGGPQVAALGYGFVAVPVAINTLVLTLA
                     ALAYNNATGRSYPHRAHAPVHPHLPPAPLALSPADFDAVLADYGEPLDIDSDDLRMIL
                     GELLARARPAASAADL"
     gene            complement(15179..17863)
                     /locus_tag="VFF98_08925"
     CDS             complement(15179..17863)
                     /locus_tag="VFF98_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63794.1"
                     /translation="MSSFPFHAARAHRTGRRGSRLLLGAGLLALTAAGAPAWAHEGDA
                     ATAANAANALEAAAPQLQPETDPAETIVVTGSHAEGRAAEKSLVPISVASAQDLARSG
                     RQNLRDALAEVLPSFQVQAGGYQGQQGAGVRGARLRGLDAKDTLVLVNGVRRHTSSLL
                     VGGASPTDLDLIPANAIERIEVLGDGAASLYGSDAIAGVINIILKKTAVTGGGFSVYY
                     GQYGQSVGDIAHMYGRTKNVQFHQGWALGDRGGFFNISANVQLQNGTNTYPGYKAANL
                     ASKTTLLYPTLADGSLDPRETSESRYRQWLGQPSSKTYSFAYNTEVPLGDLTFFSNGT
                     YAWRYSSGPGYFRTASNQTLSASTTPSPQDPFANAPYTSGTQVFPDGYLPTFDDQESD
                     FQAVAGLKGVAAGWDWSLSSGYGSDHADIYTRNSINVSAGPQYWGQRNFYDGSQTDGQ
                     LLTTLAVSRKFAAGLFQRPLTVSAGIEHRYDSFTKRAGEWLSYYAGTWVWPSGTGNAG
                     THPNLGAQGMSGNTPASAGAWSRNNIAAYIELNQQLTDKWSVDLSGRYEYFTDFGSAP
                     SGQISTRYELTPKFAIRGTFGNGFSAPTLLQEHNSTQAGSYSVDSNAQSSTYGQYIQQ
                     YTVTTNAYQATGQAIGVQALKPEHSINASLGFVARPFARTVLTVDGYLIDITNRIVTA
                     TASVTSGTALYNLLTSQGVYNVTSVSFNINGAHTFTKGFDLKLEHTDPIGRLGTLHWT
                     LASNENITSVKSFSALPAIIGSASAGTLRTLAGQFTSYYPKNISSVALRWEYGRLNLW
                     AKETYWGSTTYLGSTAATDQHQAPAFTTDASASYALRSGIRFTIGGTNVFNKRPDRLS
                     ASAQALQFISATAVSPYNSYAAFGQDGGYYYGRLDFNW"
     gene            complement(17977..18867)
                     /locus_tag="VFF98_08930"
     CDS             complement(17977..18867)
                     /locus_tag="VFF98_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63795.1"
                     /translation="MAKTIAFLACADTLPGAPHRRPDAFEHDHQVAALRAGLAGSGTR
                     LLEIDWRAPLDEICACDLALVGTPWDYTEDKAAFLARLEAIAAAGVPLWNPLPMLRWN
                     ADKLYLAELEALGAPSIPTLWHEAAGPAELAAAFTHFGGEWLVAKRRVGAGAVGQALL
                     RRGSEEALGWRADQPVMIQPFLPAIRAEGELSFIFVDGALSHALVKRAQPGDYRIQAA
                     YGGRETALHPAPADRAAAEAVLALLPFAAPPLYARIDMLRLPTGALALIEAELIEPYL
                     YPLQGPHFGRRMAQALLRRL"
     gene            18952..19212
                     /locus_tag="VFF98_08935"
     CDS             18952..19212
                     /locus_tag="VFF98_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63796.1"
                     /translation="MSKPRRYLTATMPDGYVKTIGPTSERFTHYWRIVAELENGKTEV
                     FWGHCRSLAEARRKRAPAAEAARMRGWKSFAFEIAELVETPA"
     gene            complement(19240..19638)
                     /locus_tag="VFF98_08940"
     CDS             complement(19240..19638)
                     /locus_tag="VFF98_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HZU63797.1"
                     /translation="MAGLRKRRLVAGAAALALGAAGCAGLAWAQGRDPAYAAARAAGK
                     VGELTSGYLGVVGAPTPDLRRMVEDINIRRKAVYAEKAQAQHATLEEYAFTSGCVLIA
                     STQPGEKYQAPDGSWQTRGAGAPQRDSRCP"
     gene            complement(19650..19883)
                     /locus_tag="VFF98_08945"
     CDS             complement(19650..19883)
                     /locus_tag="VFF98_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HZU63798.1"
                     /translation="MNGLRSMAARAGMVPAWRAMRAVPVLMGGALALSACISVHTPDK
                     PIVIELNINIKQEVVYRLSADAGKTIESNKDIF"
     gene            complement(19952..>22653)
                     /locus_tag="VFF98_08950"
     CDS             complement(19952..>22653)
                     /locus_tag="VFF98_08950"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HZU63799.1"
                     /translation="RDGFGARLAVVAPALRGQGCLARGGHFAGQLAVAAGQPQLAGRL
                     EAGDLACAGTGLAVARPVLDVAMGLGPGLESGAGRLVLHAGPAHGGGVQAVRLEGSAG
                     FSLRDLAPAGPNAGPNAGKAVLAADFTLGGEGVAGLAASARQLGLAGHVRLTRGGAAL
                     AAEGSLSGSGLAPSAQLVARLGSWRAAAAGSWAAPLAGAFHDGLLRAAGQSRLAGNWA
                     LRRSAGTTSLILPELRVYEAGGPYWLSASHVSVVAGRGAPVVAGRFALGGTGLPALRG
                     QLATSADGRAEAVLAMPAWAAGGASLAVPQLALRQAPGGAVELAGRVILGGPLTGDPA
                     AGPSVAGLAIPIEGRWSAGSGLVLGRRCLALGLERLALGEAELAPAGGKALRLCPGPG
                     GAMLALGPQGMRVSVRTGGVALAGEMQGAPLRVAAGALALGWPGGLEAHDVAVERGGA
                     NAGQLHLGQLALRLGPEITGRFADGEARLADAPVALRAASGRLRWAAGRLALTGGEAL
                     LEDPAATGGAAQPRFAPVRLHLADLALAGGQLVATASFSATAADRPLAQVSLAHDLAS
                     GRGHADLRLPGLTFDKALQPRQLSALAEGIIADAQGTLTGDARFDWAGGHLRSRGRIA
                     SAGLDFAAAFGPVRGVAGEVVFTDLLGLVTAPDQKLTIAAINPGIEVDDGTLTFALQP
                     GAGPQQHVLAVKGAQWPFLDGRLTLLPARLVLGASEVRHYELRVDGLNAARFLERMEL
                     SNLAATGIFDGRLPLVFDDKGGRIEGGMLVSRPPGGTVSYVGALTYRNLSPMANYAFR
                     ALRSLQYREMRIGLDGALAGEVVTRVSMRGVGQGQGASRNFLTRQIGRLPIQFDVNVR
                     APFMQLISSFRALYDPSLVADPRTLGLLDATGRPVTRPGAIQP"
BASE COUNT         3687 a         7895 c         7660 g         3411 t
ORIGIN      
        1 cgagcaggcc cgaatagggc tgctgggcgg ttgcctcgcg gctgaccttg gaattgcggt
       61 cgcggccgat atcgaaggtc tcgtcgaaag cggcggcatg ggtggggaac cggctgaccg
      121 cgccctcggc caccaccttg ccgttgacca gcaggcggat ggtgcccccg cccaccttcg
      181 acagcagcgg cagcgcgccc gcttcaccgc ccggcgccgg cacgaagtcg tagccaacgg
      241 tggccgcgcc cgccggcagc ggttcgctgg cggcaatcac ttcgtggcgc aggttgtagg
      301 cgttgttttc aaacaccagc ctgccgccct tgacgtagaa cacatagccg ccaagccgcc
      361 cgccatgggc gatcagcacg ccctcgccct tggccgcatc gcccttgaga tcgatctcga
      421 ggtgatgggc gcggcccacc agcaccggct gggcggcaac cggcaggccg gaaatatcgg
      481 gctggaagct gaagctgacc tttttggggc tgacggcaag cggcgcgcca aacggcagcg
      541 ggatcaacgg gaagacgcca ttgcggcccc cttcgcgcag gaattcggcc tgcagttcgg
      601 ccagcttggc cgggtttgcc cgggccagat ccgtcgcctc ggagaagtcc tcggtgatat
      661 ggtagagttc ccagcggtcc tgcgcggtat tgtcgttata gatgcgcccc acatccttca
      721 gcagatccca cggctggtag ctgcgcttgg ccgccgccac ccagccatcc ttgtagatcg
      781 cccggttgcc gaagatctcg aaatattgct cggtgtgggt ttcgggggca tcgggattgg
      841 tgaagctggc cagcagcgag cggccttcga agggcagctg ccgcaccccg tcgatcgtct
      901 cgggcgcgtg gatgccggcg gcctcgagga tggtcggggc gatgtcgttg acatgggcga
      961 attgcgcgcg gatccggccg ctgtcgcgcg tgtggcccgg ccagtcgacg atcagcggat
     1021 cgcgggtggc gccaaaatgg ctggccacct gcttcatcca ctggaacggg gtggatgtgg
     1081 cccaggccca gccggccgca taatggttgg catattccgg cccgcccagt tggtcggcat
     1141 gggcggcgat ggtggcaaaa tcggcggggg cgccgccggc cttggcctgt tcattggcaa
     1201 tggcgccctc gatatcgccc tcggcgctgc cgccattgtc gcccacgata tagagcacca
     1261 gcaggttgtc gccgcccggg cggctgcgca gcgtgtcgat cagccggccc acttcggcat
     1321 cggtctgttc gagataggcg gagaacacct ccatctcatg cgcatagagc tgcttctgcg
     1381 cggctgtcag gctgtcccag gcgggcaggc catcggggcg cggggtcagt ttggtgtccc
     1441 tggggatgat gcccagccgc ttctggcgct cgaaggcggc ggtgcgatag gcatcccagc
     1501 cggcatcgaa gcggcccttg ttccgcgcga tccattcctg cggcacatga tgcggcatgt
     1561 gcacggcgcc cgtggcgaaa tagaggaaat agggcttggc cgcagcggcg gcattatgat
     1621 catccaccca gccgatcgcc cggttcacga tatcggtggt gaagtgatag ccctgttcgg
     1681 gcgtggccgg cagcggggtt gcggtggtgt cgttgtagag atgcggctcc cactggttgt
     1741 catcgcccga catgaagccg tagaaatgat cgaagccaag gccggtgggc cagtggttga
     1801 aggggccggc cggcgtcact tcccagcgcg gagtgttgtg ccacttgcca aaggcggcgg
     1861 tcgaatagcc gttcagctgc aggatgcggg cgatcgagcc ggtctcctgc ttccagatgg
     1921 aattataggc cggaaagccc gcggcgatat cggccagatt gccaaagccc acctgatggt
     1981 ggttgcgccc cgtgagcagg gcggcgcggg tgggcgagca gatgccggtg acgtggaagc
     2041 ggttgtaacg cagcccctcg gcggcatagc gatcgaggtt gggcgtgcgc gcgccgccgc
     2101 cgaaagtggt ggtggcggca aagccggcat cgtcgatcag caccagcagc acgttgggcg
     2161 cacccttggg cgcctcggcc ggcctgggga aggcgggcac ggattgcgcg cggctctgcg
     2221 cgatcttgcc ggcaaaagct tcgccctcgg ggatatgctg ggccatggcc tgcccggcca
     2281 gcgccgtggc agacaggagc agggccgcac cggcaaggcg gcggcgggcg gacgaaaggc
     2341 gcaaaagagg catcggttgt tccttgaaga cgcgctggcc ggggcgccgc gccagggcgg
     2401 caccggcagc ggtgcgttgc gggaaaggga gatgccctgg cgtggtcgcc tcgggcagga
     2461 agctgggggg ccggaagctg cggggcgggc ccgccgcggg ggctcagccg gcggcggccg
     2521 cgctggccgg atcgcggccc gcgcggatag cggcgcccag ccgggcgatc accggcgcgg
     2581 tgtgggagag atagagcgtg aagccctcgc acagatagtt gagccccggc tcgcccgcgg
     2641 cagtggtggc aaagcggtgc ttggggcagc ccccccagca cgcgccaaga tgcgggcagg
     2701 cgcggcactg gccgggcagc gtttcatatt tcgccttgcc gaaagcctgc gcccgcgcgc
     2761 catcaaccat ggccaccagg ctgtcgctgg ccagattgcc cagcttgtat tcgggataga
     2821 cgaaatggtc gcaggcatag acatcgccat tgtgctcggc caccagcgcc cgcccgcaca
     2881 ccggctgatg atggcacaca acccccggcg caccgaggtg ggcggccagc gtccattcga
     2941 aattcatcac gaaaacgcgg cccacatcgt gctggcgcca ttcatcaaac acggcattga
     3001 ggaactgccc ccaggccgcc ggcgccacgg aaaaaggcgc cacggccgca tctgccgcgg
     3061 cccccgggcc attcagcgcc agcccctggg ctgcggcctg cgcgccggcc acccgctcga
     3121 ccacgggggt gaactggacg aattccacct gcgcctcatc acgcaggaag cgatagacct
     3181 cgagcggggc ctgcgaggaa tgcgcatcga cacaggccag cacattatag gcaacgccgg
     3241 ccgcctgcag gtggcgcagc gcggccatca cctgggcatg gctgggcttg ccattggcat
     3301 agcggcgata gcgatcgtgg acagaggccg ggccatccag gctgagcccc accagaaagc
     3361 cctgttcggc gaaaaaccgc gcccattccg catcgatcag cgtgccattg gtctggaagc
     3421 tgttttcgat ggcgcgcccg gcgccatggc gcttttgcag cgccacggcc tgccggtaga
     3481 aatcgatccc cagcagcgcc ggctcgccgc cctgccaggt gaactgcacc ggcgcctggc
     3541 gcggcgttgc ggcgatggtc tggcgcacat aggcttccag cgcctcggcc gacatgcggc
     3601 gcgcctgttt gcgctcggca aacagcgcct ctttttccag atagaaacaa taggtgcaat
     3661 caaggttgca ttgcggcccc gacggcttgg ccatcaggtg aaagggcgcg gcggccgagc
     3721 gctgcaagcc gaggcgggaa gcaattgcca tgcgaaaaga cagaccctgt tgcgatgcgc
     3781 acagggctgg ggccttcgcg cgttatatgt ctacttttat aatagacaat gaacgggtca
     3841 agccgggtgg gccggccagc accgctagat taacttcgac gggggcaatc aagccttgtt
     3901 tggcttaacc ctacttgagc agtagagata tgcctgtcga caggtgtgga ggcccacacc
     3961 gccggccgct gatgcaccgc gcggcttgcc ccacgatgcc agatgccaaa gcgtgcacaa
     4021 gctggggcga cttcgccagg ccgaggggtt cttgccctcc tgcacacccc gccccgcagc
     4081 cagacagctc tgccccagac ggctcttgcg ggcttctcac acccaaacgg acacaggaga
     4141 ttttcccatg gccactgtcg ttgcctctta tcccaacgcc aaggatcccg cctcgccgct
     4201 cgacatccgc ccggttgccg gccgcattgg cgctgtcatc cacggcatcc gcctgggcgc
     4261 cgacctggat gacgcgacca tcgccgcgat cgaggcggcg ctgatccgcc acaaggtggt
     4321 gttcttccgc gatcaggccc acctgaccga tgccgagcac gaggccttca cggcgcgctt
     4381 cggcgatccg gtggcgcacc cgacggtgcc ggtggtggaa ggcagccggt atctgctcga
     4441 actcgacagc aaggaaggct atgccgcctc cagctggcac accgatgtca cctttgtcga
     4501 tgcctatccc aaggcctcga tcctgcgggc gctgaccatt cccgaggccg gcggcgacac
     4561 gctctgggcc aatggcgaga ccgcttacga tggcctgccc gaagcattgc gccagctggt
     4621 cgacaacctc tgggcggtgc attcgaacga ttacgactat gccgccgtgc tcaaccaccg
     4681 caccgatgag gacagcgagg tgctgaacga gcgcaagaag ttccagaaga gcgtgtttgc
     4741 cagcacgatc tatgaaaccg aacatccggt ggtgcgggtg catccggtgt cggggcagcg
     4801 cagcctgctg ctggggcatt tcgtgcgccg cttcgtgggc ctgaacgagg tggattcggc
     4861 ccgcatcctc aatgtgctgc aggatcacat cacccggccg gaaaacaccg tgcgctggcg
     4921 ctggcagccg ggcgatgtgg ccttctggga caaccgcagc acgcagcacc gcgcgattgc
     4981 cgatttcggc ctgcagcgcc gcaccctgcg ccgcgccacc gtgcatggcg aggtgccggt
     5041 gggcatcgat ggccgccaaa gccgcaccac ccgccaggaa aaggccggcc tgacacccgc
     5101 ggcctgaggt gctgagccca aggggcggag cgcccgtgcg cgccgcccct tgcggcttac
     5161 ctgaccccgt agaccagcat ggcattgccc ggcatcttgc cggcaaagcc atagcccgtt
     5221 tccaccagca gcaggccatg ccaggcaatc ggcgccgcgc cgccgctcat cgagccgcca
     5281 tgggcgggct gcccgctgac ggtggcgaca gggccggtgg tatcggcatc ccacagcacc
     5341 gcgccgctgg cggcatcgaa gatgcgcaga tggccatcgt tgctgcccgc cagcaccagc
     5401 tgcgaggtgg cggtgatcgc ggccgaatag cccggatggc acagcggccg atcgccgcac
     5461 acgcctgccg gtgccggcgc ctgccacacc cgggccccgg tggccacatc cagcgcgaac
     5521 aggcccggat gcggcggctc ggcataggtg cggccatccg gcgcatcgct gatcggcacg
     5581 tagagcaggc cattgctggt ggccatgccg aagtggatgc cgcccactac cccgccgcgg
     5641 ccgatctgcg cctgccacac cagccggccg gtatcgggat ccagcgcata ggccgtgccg
     5701 gatttctgcc cggccagcag ataatcatgc ccgccaacgc cgcgcgccag caccacgccg
     5761 gcgccaaaat catagtccgg gcccttttgc gcggggcaat tggcggtgtc gcggtcctcg
     5821 cacgatccgt tccacacatc gcccgcgcgc gcctgatacg accagcggat cgccccggtg
     5881 ccaaggtcca tcgccacgat ggcatcgctc atcggcgtca ccggatcgga ataattgtcg
     5941 ccggtgtcga gatagagcag gccgcgcgcc ggatcgatcg ccggcgtgtt ccagatcgcc
     6001 acccccgatg ggccgaagac ctgcacgcca tcggcattca ggccgcgcgg ctggggcttg
     6061 tcggtgaaat aggtgcgcca gcgagtggca ccggtgcgcg catccaccgc cagcagcgat
     6121 ccgcggaagg tgcagcagga atagcccggg ctggcagcag ccccttcctc cagcgacgag
     6181 accgggatgt agagcgtgtc gcgatagagc gccggggtgg cggtcagcgt ggtgctggga
     6241 tggtcatcgg cgtgcaggct ccacaccctg gtgccggtgg tggcatccag cgcatagaca
     6301 ttgccgacca gatcggaaaa atagaccagc ggatgggcgc cgtgcccgcc cggcgtccac
     6361 ggcgccacca cgggcgccgt gcgcacttcg ccgcgggcgt ggaagatcca gcgcgcgcag
     6421 cccgtttcgg catccagcgc atagacagag ccatcatggc tgccgacaaa gatcgccccg
     6481 ccggcaatcg ccggctgcga gcgggcgcgc agggcaccgg gaaagccgat ggcccatttg
     6541 agatgcagct tgcccacatt cgcgcggtcg atcccggcca gctcatcggc ctcgcgatgc
     6601 gtgttggcca gatccagccc ccagccggca aaggccggcg gcgcgcggtc gtcgaaccag
     6661 ttggcctttg gggcacacat gcgcgggggc ggggcggtat gcgtgcccag cgcctgatgg
     6721 gtgacatatt gggccacggc ccgcttctgc ccatcatcaa gcgaggcggc catcggctgc
     6781 atcacccctg cggtcaaggc gtgatagacc gcctcgggcg tgagctgcgc aatgatgtat
     6841 ttctgcggcg cgcggttgag gccctgttcg tggcagcctg aacagatggt ctggtagacc
     6901 tccttgccct tggcctgcac ggccgcatcc agccgggcgc cgggatcgcg ataggcatcg
     6961 gcgggcggcg ctgaactgtg catccaggca ccatcatgcg tgtcctgggc cagcgcggcg
     7021 ccaggcgtgc caggcatggc cccggccagc atcatggcag ccgccaggcc aagccagcct
     7081 gccttgcgca gtggcgaatc cggcattcgt gctctcctcg tcctgcctgt ctgttttggt
     7141 gcaggcgtat tattcacgaa tagccggcag cgccgttgcc gcagctaacc atttggaggg
     7201 gggcacgcag atccagccct tgcggccagc cgggcagggc ctagaagcgc gacagatcca
     7261 gcgcctcact cggccccagc cagaggacgt gggccgtgtg atcggccaga tcatcgcccg
     7321 tattggcgtg catgaagatg gtgagcgcgc cgcgatggcc cagcagccac atcgtcaccg
     7381 gcgcaaaaga agcaaccggc acggtgatct ggcagctgcc gcgcggatgc ggccccaccg
     7441 gctgcagatg aacgcgcccc atccgcgttt cgggaaaagc ggcgatgatc gcctgcccca
     7501 gcgcccctgc aaccccggcc tgatcggggt cgaaatagat atgggcatga aaatccctga
     7561 tcgtgggcat gaggcctccg gttgtgcggt ttctgccctg cttgcggtag gccagcccat
     7621 cgccagaggc aatgcgctga tccggccatc gcggcagggc gccttctcct gcccctaatg
     7681 cgccgcaaga tgcgcggcgc gatggctgct gcggccgggg cgcgggggcc ggtgttcctc
     7741 ggcgcggaca cgatggcgca ctttgagcag atcgtggcgg gtgacgatgc cgatcacctg
     7801 cccgctgcct ggcgccacca ccgggatccg cccgatgccg ctttccagga tgagatcggc
     7861 gatggccgcc gccggggttt ccggcgtggc gcagggctgc gaggcatcgg acagcctgtc
     7921 ggcgagcgtg gcgccgggct ctgcgccgcc ggggcccagc gccagcgcat ccccgcggga
     7981 gaccagcccc agcaggcgcc ctgccgcatc caccaccgga tagctgcgat ggcccgccgg
     8041 cccggccaga aacgcggccg cctcggccat cggcatggtg ccgggcaggg tctgcgggtc
     8101 gggtgtcatg atcgccccgg cctggatcag gtccagcgga tcgaccgtgt attcctgcag
     8161 gatatgccgc ccgcgccggg cgatcttctc ggtaaggatc gagcggcgca tcagcagcac
     8221 gctcaccgca tgggcgccgg ccgcggccgc cagcgtcagc ggcagggctt ccagatggcc
     8281 ggtcagctcg gcggcgaaca gcgcgccggt cagcggcgcg cgcatggccc cgctcatgat
     8341 gccggccatg cccagcatcg cccacacccc ggcgccgccc ggcagcagca gccccaccag
     8401 cgcccccagc gcgccgccca gcatcagcaa aggcgccagc acgccgcccg acgtgcccga
     8461 tcccagcgcc accagccaca ccgccgcctt catcaccagc agcagcgccg tggcgcgcgc
     8521 cgtcatcccg ccatcgagca gcgcctggat attgccatag cccgccccca gcacgcgcgg
     8581 ctccaccagc gcccccagcc ccactgccag cccgcccagc gccggccacc acatccagtg
     8641 cacgggcagg cggtggaaac catcctcgat ccggtagagc agcgccgaca gcagcccggc
     8701 cagcaggccc gccagcgccc ccagccccag cgccgcggcc agcggccagg cccccgccgg
     8761 cacgcccagc gctgccggaa acatcggccc ggcgccaatc caccccggcc gcaaggcact
     8821 ggccaccagc acggcgatca ccaccggcac gaagctgcgc ggcttccatt cgaacagcag
     8881 cacctccacg gccagcagta ccgcggccag cggcgtgccg aagatcgccg tcatgccggc
     8941 ggccgcgccg gccaccagca gggtcttgcg ctcggccgcg ctgaggtgga aaccttgcgc
     9001 gaacagcgag ccgatcgcgc cgccggtcat gatgatcggg ccctcggcgc cgaaggggcc
     9061 gccgctgccg atcgagatcg ccgaggacaa gggcttgagg atcgccacct tcagcgacag
     9121 ccggctttcg ccatagagga tcgcctcgat cgcctcgggt atgccatggc cgcggatctt
     9181 ctccgagcca aagcgcgcca tcagcccgac aatcacgctg cccagcaccg gcagcgccac
     9241 cagcagcggc agcggcgcca tcatcgccgc cggcagggtg gcaaggcggg catgccagaa
     9301 caggttggtg gccagggcta tcagccccag cagcgcccat gccccggccg cgccccccgc
     9361 ccccaccggc accgccgcca gcgccagcag cagcatgcgc ccatcggccg aatgatcgcg
     9421 caggcgcgca ggagatgccg ggggatggac aaaggtcatg ttcgctccga gggtgatcac
     9481 ttacgcgtca atatatcgta ttacgacata aatcaacgga gcctgcaccc gatgcccgac
     9541 agcgaggccg acctgaccga tgccgattac gccgtgctgg ccgctttccg ctttgcgctg
     9601 cgcagttttc tggccttcag cgagggcaag gcggcggcct gcggcctcac cccgcagcag
     9661 caccaggccc tgctggcgat ccgcgcggcg cccgaggccc gcgccaccat cggctatgtc
     9721 gccgagcggc tgatcctcaa gccccacagc gccagcgggc tggtcgagcg gctggaggga
     9781 ctggggctct tgcggcgcgc gccctcgccc accgacaggc gccaatccgt gctgtccctc
     9841 acgccggagg ccgagaccgt tctgcgcgat ctgagcgcca cccatcgcga ggaaatccgc
     9901 cgcctgcgcc cgctgctcac cggccttttg gcacatatcg gctgaatggc gcgccgcaac
     9961 ccgccccgca ggcggcggcg cgccatctgg ccggtcagcg gcggccgtgc aacacgctca
    10021 cctcatcgcc catcggcacc agcgaatcct cgtcgaacac gctgattttc cagccctgcg
    10081 cggtgcggac ataggtgatg tcgtagcagc agtggacaaa cacgctggcg ccgccgcgcc
    10141 cctcggcctt gccgatcaca tagccgcgcg ccttggcctc atcgccggcc agatggttga
    10201 tgtggaaatt ggtgacatgg tggcagtgat agcgggtgtc ggcaaacacg ccctggaaga
    10261 aggcgcggat ggcgccatgc ccgcgataga cctccagccc cagatccgcg acatcgaaca
    10321 gcgcatcggg agtgaagcag gccagcagcc cgtccatgtc cgccagcgtg tcgacggcgg
    10381 tgtaatattg cagcagcacc ctttccagct cggcgcggtc ttccagttcc ctgatccgct
    10441 gttccagact gtgcatggcc ttctctcctc tttgttatgg tgtgggtatg gtgccggtca
    10501 aaagggggcg ggatagcagg cgccggcgat ctcctcgtag accgccgccc aggattgcgg
    10561 ggcgagcgtg tcggagaagg tgatctgccc tgcggcgatc gcccggttca gcgcatcgag
    10621 cgcctgggcg gtatcgggca cttcgccttc gtaaaggatc agatagcggt agctgtcctg
    10681 ctgcgtgtcc agcggctgca ccaggcgcat ccgcttggcc cggacaaagc cgccatggcg
    10741 caccatggcc ggcagatgct cctgcgtgtg ccaggcgctg aaggccgcat cctggccgtc
    10801 tgccgcattc accaggcaga caaaactgaa tttgcccatc gcgggtctcc ctgccggtgg
    10861 cgcctcaacg gcggccttcg agatagtcgt tcagcacttc gtgaaagcgg cggatgcggt
    10921 cttcctgatc gctgagatag gcatcctgat agccgcgcga gtgaaagccg ttctgctggc
    10981 tgaccgccac ggccagatcc tgatcgagga aattgcccag cggatgcgcg ctgccggcgc
    11041 cctggccata ggtgaaggtt tcgtgggcgg ccggggcaaa ggggcgcgca ccgaagatcg
    11101 tctgcacttc gtcgcggccg gccaccggcg gcacgaaata ccagtaatcg aaggtgcacc
    11161 attccggatc gcgcggatca ggctcggtgc gatagaggtg caggccatcg caggtggccg
    11221 tcagcgtcac attgggaaac agcgtgtggt ggaaataatc ggtcagctcg tcgtcatgca
    11281 ggctggcgaa atgggcatgg ccgcgcgccg ggcccagccg gcgcttctgg cgctgcaggg
    11341 ccagccggcc atcgcgcgag cggccggcga aatcggccgg atccagatcc catgcgcgca
    11401 gcatgtcttc ccaggccggt tccacctcgt tgggatgggt ggcgcgcagc gagggctggc
    11461 cctgctcgat catccggttg tggccggccg gatgcatctc gaaaacggtg ttgcggtaat
    11521 cctcgtcgac gatctgctgc atctgcggat ggacggtggg caggtgatag ctctcgttga
    11581 aattgtcggg ggcgaacttc cagttggtgt tcacccgggt ggtggtccac agcacgcggg
    11641 tcatctgctc gatgccgcgg ctggccagca gggcggggat cggcgccaga taatcggcca
    11701 gcgcgggggc ggccggatcc atgctgtacc aggcaaagcc gccccagctg gcgcaaggaa
    11761 tttccttcag ccccaccttg ccgcaaggat tgccgcgcgc gaaattctcc ggatcctggg
    11821 cctggcgcac caggccatcc agcccatagc gccagccatg ataggggcag gcaaagccat
    11881 gcggcaccga tccggcctgg ccctgcacca gctggttgcc gcggtggcgg caggcgttgt
    11941 ggaaggcctt gatcgcgcca tccggctggc gaacgatgat caccgattcg cgcaggaaat
    12001 catgcacgac ataatcgccc ggctcttcca gctcggccat cagcccgccg atatgccaga
    12061 tgcgcgtcca catatgctcc cattcggcgc gggcgaaggc gggggaatag tagcgctcgc
    12121 cggtgatcgg atcgtggcgc aggcgcacgg gcgggcccgg cggcgccagc ggataggcag
    12181 ggcgcagcgc agcggcatca gtggacatca cgtctctccc cggtgtgcgg acgtggtgca
    12241 ggttattgta cacccgtccg actgattgaa cacttgtact ataccgcgcc cgcaagccca
    12301 tgcaagaggg ccaggcaggc atgctgcagg catggctggc ggcgggcttg ttccgcttgc
    12361 tggccatggt aagggggcaa ggatggcgcc accgatccgc aaaacccgac tggacccgga
    12421 aggcgtgcgc cagcgcattc tcgacgcggc cgaaagcctg tttgccgagc gcggctatgc
    12481 cgccaccccc ctgcgcgacg tggccgcgat cgccggcgtg ccggtcagcc tgatcaccca
    12541 ccatttcggc accaaggaag atctgttcaa gcaggtgatc gcccggcgca tccacgatca
    12601 tgtggccagc atgcgcgccc agctggaaac cgcccgccag caggccgccg gcgcgccgat
    12661 cggcgcagag gctttcgtca gggcctatat cggcccgatg atcagccttt cggtgcatgg
    12721 cgggccgggc tggaagaact atgcccggct gctggggctg gcgatgaaca gccggcaata
    12781 tgaggaattc ctgaaaccga tgatcgaggt ctacgatccg gtcaacgcgg aattcgtggg
    12841 cgagctgcgg cgcatctatc ccgatgcggc gctgcgcagc ctgcattggg gcatgtattt
    12901 cctcaattcc accatcctgc atgtgctggt cgaggcgggc atggtcgatc gccagtcgca
    12961 ggggttgtgc cgctcggccg agctggaggc cattttggag gagatggtgc ccttcttcgc
    13021 cgccgccttc gcgcagcggc tgggcggcaa ggccacgccg ctgcccgagg ccaacctccc
    13081 catctgatgg cccctgcaag gcagcggccg ggccctgccg gggcgggcgt tttttcggtg
    13141 cgcctaaaga ttcattttgg ttgcatgtta tatcgcatga cgatattccg cacatcaaat
    13201 gcatctttaa gagtcgcccc tcaacgcccc tgcccaaagg atctgcccat gctcaccgcc
    13261 gaacagaccg ccatcgtcaa ggccactgtg ccgctgctgg aaaccggcgg cgaagcgctg
    13321 accacgcatt tctaccgcat gatgctggcc gaacaccccg aagtgcgccc gctgttcaac
    13381 caggcgcatc aggccagcgg cacccagccg cgcgcgctgg ccaatgccgt gctgcaatat
    13441 gcccgccata tcgacgatct gggcgcgctg ggcgccctgc cggcgcagat catccagaag
    13501 catgtggcgc tgcagatcct gccggagcat tatccgatcg tcggcggctg cctgctgcgg
    13561 gccatccgcg aggtgctggg cgcagagatc gccaccgatg cggtgatcgc cgcctggggc
    13621 gcggcctatg gccagctggc cgatatactg atcggggccg agggcgccga atatgatcgc
    13681 ctggccgccg cccccggcgg ctggcgcggc acccgccgct tccgcattgc cgccaaggtg
    13741 cccgaaagcg cggagatcac ttccttccat ctggtgccgg ccgatggcgg gccggtggtg
    13801 gcgcatcagc cggggcaata tctgggcctg cgcctcacca tcgacgggca ggaagtgcgc
    13861 cgcaactatt cgctctcgca agcggccaac gggcgcgatc tgcgcatcag cgtcaagcgc
    13921 gagccgggcg gtgtcgtctc cacccatctg cacgatgtgc tggcagaagg cgacgagctc
    13981 gatgtcttcc cgcccgccgg cgccttcgtg ctgcgcccgg gcagcggccc tgtggcgctg
    14041 atcagcggcg gggtgggcat cacccccacg ctggcgatgg ccgaaagcgc gctggcgcag
    14101 gggcgcgatc tggtgttcat ccactatgcc cgcaacccgc aggtgcaggc ctttggcgcc
    14161 acgctggcgc aatgggcgcg ccagcacccg ggctttaccg cccatgtggt gctggaagaa
    14221 ggcgcggcca cgggcgcgcc ggcagggcgg cccagcctcg cccagctgag cgcctgggtg
    14281 ccggtggaag gcgatgccta tgtgctgggc cccaagccgt tcatggcctt cgtcaaccgc
    14341 gcgctggccg agctgggcgt ggccgaaggg cgccgccatt tcgaattctt cggcccggca
    14401 gaagcccttg cctgatccgg cgggggccct tgcggcagat cggcggatgt tgcgctttcc
    14461 ggcccttgcg gccttcctgc cccacagccc gccggcgggc ctgcgcgaga cggcgcgggc
    14521 cgcggccggc gcgggcctgg gcatcgcgct gacagcgctg gtgagcacgc tgctcgctgg
    14581 cgggcatggc gccctgccgc tgctgatcgc gccgatgggg gcatcggcgg tgctgttgtt
    14641 cggcgtgccg gccagcccgc tggcgcagcc ctggtcgatc atcggcggca atctggtggc
    14701 agggatcatt ggcgtggccg ccgctcacac catcgccgcg ccgggccttg ccgcggcagt
    14761 ggcggtggca ctggcgctgg cggcgatgac ggtgctgcgc tgcgtgcatc cgcccagcgg
    14821 cgcggtggcg ctcaccgccg tgctgggcgg gccgcaggtg gcagcgctgg gctatggctt
    14881 tgtggcagtg ccggtggcga tcaacacgct ggtgctgacg ctggcggcgc tggcctacaa
    14941 caacgccacc ggccgctcct atccgcaccg cgcccatgcg ccggtccatc cgcacctgcc
    15001 gcccgcgccg ctggccctgt cgccggccga tttcgatgcg gtgctggccg attacggcga
    15061 gccgctcgac atcgacagcg acgacctgcg catgatcctg ggcgaattgc tggcgcgggc
    15121 aaggcccgca gctagcgctg cggacctctg atggcaccgg attctggctg gaacgggctt
    15181 accagttgaa atccagccgc ccgtagtaat agccgccatc ctgcccgaaa gcggcatagg
    15241 aattatacgg cgagacggca gtggccgaga taaactgcaa cgcctgcgcg ctggcggaga
    15301 gccggtcggg ccgcttgttg aacacgttgg tgccgccgat ggtgaagcgg atgccgctgc
    15361 gcagggcata gctggcactg gcatcggtag tgaaggcggg cgcctggtgc tggtcggtgg
    15421 ccgcggtgct gcccagatag gtggtggagc cccaataggt ttccttggcc cacaggttca
    15481 gccggccata ttcccagcgc agcgccaccg agctgatgtt cttgggataa tagctggtga
    15541 actggccggc cagcgtgcgc agcgtgccgg cgctggccga accgatgatc gccggcagcg
    15601 ccgagaagct cttgaccgag gtgatgttct cgttgctcgc cagcgtccag tgcagggtgc
    15661 ccagccggcc gatgggatcg gtatgctcca gcttcaggtc gaagcccttg gtgaaggtgt
    15721 gcgccccgtt gatgttgaag gagacgctgg tgacgttgta gaccccctgc gatgtcaaca
    15781 ggttatacag cgcggtgccc gaggtgaccg aggccgtggc ggtgacgatg cggttggtga
    15841 tgtcgatcag atagccatcc accgtcagca ccgtgcgggc aaagggccgg gcaacaaagc
    15901 caaggctggc gttgatgctg tgctccggct tcagcgcctg cacgccaatc gcctggccgg
    15961 tggcctgata ggcgttggtc gtcaccgtat attgctggat atactggcca taggtgctgc
    16021 tttgggcgtt gctgtccacc gaataggagc cggcctgggt ggaattgtgt tcctgcagca
    16081 gggtgggggc ggaaaagccg ttgccgaaag tgccgcgaat ggcgaatttg ggcgtcaatt
    16141 catagcgcgt ggagatctgg cccgagggcg cgctgccgaa atcggtgaaa tattcatagc
    16201 gcccggagag atcgaccgac catttgtcgg tcagctgctg gttcagctcg atataggcgg
    16261 cgatgttgtt gcggctccac gcgccggccg aggcgggcgt gttgcccgac atgccctgcg
    16321 cgcccagatt ggggtgagtg ccggcattgc cggtgcccga cggccacacc catgtgccgg
    16381 cgtaatagga cagccattcg ccggcgcgct tggtgaagct gtcgtagcgg tgctcgatgc
    16441 cggccgagac ggtgagcggg cgctggaaca ggccggcggc aaacttgcgc gataccgcca
    16501 gcgtggtcag cagctggccg tcggtctgcg agccatcata gaagttgcgc tggccccaat
    16561 attgcgggcc ggccgaaacg ttgatgctgt tcctggtgta gatatcggca tggtcgctgc
    16621 catagccgct gctgaggctc cagtcccagc cggcggccac gcccttcagg ccggcgactg
    16681 cctgaaaatc gctttcctga tcgtcgaacg tgggcagata gccatcgggg aacacctggg
    16741 tgccgctggt gtagggcgcg ttggcgaagg gatcctgcgg gctgggcgtg gtgctggcgc
    16801 tcagcgtctg gttggaggcg gtgcggaaat agcccggccc cgaggaatag cgccaggcat
    16861 aggtgccatt gctgaagaag gtgagatcgc ccagcggcac ttcggtgtta taggcaaagg
    16921 aataggtctt cgaggaaggc tggcccagcc actggcgata gcggctctcg ctggtctcgc
    16981 gcgggtcgag cgaaccatcg gccagtgtgg ggtagagcag cgtcgtcttg ctggccagat
    17041 tggccgcctt gtagccggga taggtgttgg tgccgttctg cagctgcaca ttggcgctga
    17101 tgttgaagaa gccgccgcga tcgcccagcg cccagccctg gtggaactgc acgttcttgg
    17161 tgcggccata catatgggcg atatcgccca cgctctgccc atactggcca taatagacgc
    17221 tgaagccgcc gccggtgacc gcggttttct tgaggatgat gttgatcacc ccggcaatcg
    17281 cgtcagaacc atagagcgag gccgcgccat cgcccagcac ctcgatccgc tcgatggcat
    17341 tggccgggat caggtcgaga tcggtgggcg aggcgccgcc caccagcagc gaggaggtgt
    17401 ggcggcgcac gccattgacc agcaccagcg tgtctttcgc atccagcccg cgcagccgcg
    17461 ccccgcgcac gcccgccccc tgctggccct gatagccgcc cgcctgcacc tgaaagctgg
    17521 gcaacacctc ggccagcgca tcgcgcaggt tctgccggcc agagcgggcc agatcctgcg
    17581 cgctggccac ggaaatcggc actaggctct tttcggccgc gcgcccttcg gcatggctgc
    17641 cggtgaccac gatcgtctcg gccggatcgg tttcgggctg aagctgcggc gcggcggctt
    17701 ccagcgcatt ggcggcattg gcagcagtgg cggcatcgcc ctcatgcgcc catgccggcg
    17761 cgccggccgc tgtcagcgcc agcaggccgg cgcccagcag caggcgggaa ccgcgccgcc
    17821 cggtgcgatg cgcccgcgcg gcgtggaacg gaaaactgga catgcaaacc cccttattaa
    17881 atccacttaa tccctatgag atatgtagag ttaatgaagg aacatttttt tgcccggcgc
    17941 aaaggcggcc ttgcgcgccc tttggcccag ccctgcctag agccggcgca acagcgcctg
    18001 ggccatgcgc cggccgaaat ggggcccttg cagcggatag agatagggct cgatcagttc
    18061 cgcctcgatc agcgccagcg cgccggtggg caggcgcagc atgtcgatgc gggcatagag
    18121 cggcggcgcg gcaaagggca gcagcgccag caccgcttcg gccgcagcgc ggtcggcagg
    18181 ggccgggtgc agcgctgtct cgcgcccgcc ataggccgcc tggatgcgat aatcgcccgg
    18241 ctgcgcccgc ttcaccagcg catggctgag cgcgccatcg acgaagatga agctcagctc
    18301 gccctcggcg cggatcgccg gcaggaaagg ctggatcatc accggctgat cggcccgcca
    18361 gcccagcgcc tcctcgctgc cccgccgcag cagcgcctgg ccgacagccc ccgcccccac
    18421 ccgccgcttg gccaccagcc attcgccgcc gaaatgggta aaggcggcgg ccagctcggc
    18481 cggccccgcc gcctcgtgcc acagggtggg gatggagggc gcgcccagcg cttccagctc
    18541 ggccagatac agcttgtcgg cattccagcg cagcatgggc agcgggttcc acaagggcac
    18601 gccggccgcc gcaatcgctt ccagccgggc gaggaacgcc gccttgtcct cggtataatc
    18661 ccagggcgtg ccgaccagcg ccagatcgca ggcgcagatc tcgtccagcg gcgcgcgcca
    18721 gtcgatctcc agcagccgcg tgccgctgcc ggccagcccc gcgcgcaggg cggccacctg
    18781 atggtcgtgc tcgaaggcat cggggcggcg gtggggggcg ccgggcaggg tgtcggcgca
    18841 ggcgagaaag gcgatggttt tggccatggc ggcggactag cgccgttgcg cggcagcggc
    18901 aaccacacga tacgttgcgc ggcagcggca accgggcgat gaggggcgac catgtcgaaa
    18961 ccgcgccgct acctcaccgc caccatgccc gacggctatg tcaaaaccat cggcccgacg
    19021 agcgagcgct tcacccatta ctggcggatc gtggccgagc tggaaaacgg caagaccgaa
    19081 gtgttctggg gccattgccg atcactggcc gaagcgcgcc gcaagcgggc gccagcggca
    19141 gaggctgcac ggatgcgcgg ctggaaaagc tttgccttcg aaattgccga gctggtggaa
    19201 acgccggcct gaatcaaggg ccgcgcgaat cgcgcgctat caagggcagc gcgaatcgcg
    19261 ctggggcgcg ccggcgccgc gcgtctgcca gctgccatcg ggcgcctgat atttctcgcc
    19321 cggctgggtg ctggcaatca gcacgcagcc cgaggtgaag gcatattcct ccagcgtggc
    19381 atgctgggcc tgcgccttct cggcatagac ggccttgcgg cggatgttga tatcttccac
    19441 catgcggcgc agatcaggcg tgggcgcgcc caccaccccc agatagccgc tggtcagctc
    19501 gcccaccttg cccgccgcgc gcgccgccgc ataggccgga tcgcgcccct gggcccaggc
    19561 caggccggca cagcccgcag cgcccagcgc caatgccgcc gcccctgcca ccaggcgccg
    19621 tttcctcaag cctgccatgc ccatcgccat cagaagatgt ccttgttgct ctcgatcgtc
    19681 ttgccggcat cggccgacag gcgatagacc acttcctgct tgatgttgat gttcagctcg
    19741 atgacgatcg gcttgtcggg cgtgtgcacc gaaatacagg ccgacagcgc cagcgcgccg
    19801 cccatcaaca ccggcaccgc gcgcattgcc cgccatgccg gcaccattcc cgccctggcg
    19861 gccatgctgc gcaaaccgtt catgggccga tgcatcgcag ccttggcggg caaggtcaac
    19921 tcgtggctcg tcataggcaa tcctcgctga cttaaggctg aatggcaccc ggccgggtca
    19981 ccggccgccc tgtcgcatcc agcaggccca gcgtgcgcgg atcggccacc agcgagggat
    20041 catacagcgc gcggaacgag ctgatcagct gcatgaaagg cgcacgcacg ttgacatcga
    20101 actggatcgg caggcggccg atctggcggg tgaggaaatt gcggctggcg ccctggccct
    20161 ggcccacccc gcgcatgctg acccgggtga cgacctcgcc ggccagcgcg ccatcgaggc
    20221 cgatgcgcat ctcgcgatat tggagcgagc gcagcgcccg gaaggcataa ttggccatcg
    20281 gcgacaggtt cctgtacgtc agcgcgccga cataggaaac cgtgcccccc ggcgggcgcg
    20341 acaccagcat gccgccctcg atgcgcccgc ccttgtcgtc gaacaccagc ggcaggcggc
    20401 catcgaaaat gccggtggcg gccaggttcg acagttccat ccgctccagg aagcgcgccg
    20461 cgttcagccc gtccacgcgc agctcgtaat ggcgcacctc gctggcgccc agcaccagcc
    20521 gcgcgggcag cagcgtcaac cggccatcga ggaagggcca ttgcgccccc ttcaccgcca
    20581 gcacatgctg ctgcgggccg gcgccgggct gcagggcaaa ggtcagcgtg ccatcatcca
    20641 cctcgatgcc cgggttgatc gccgcgatcg tcagcttctg gtcgggcgcg gtgaccaggc
    20701 ccagcaggtc ggtaaacacc acctcgccgg caacgccgcg caccgggccg aaagcggcgg
    20761 caaaatccag cccggcagaa gcgatgcgcc cgcggctgcg cagatggccg ccggcccagt
    20821 cgaaccgcgc atcgcccgtc agcgtgcctt gcgcatcggc gatgatgccc tcggccagcg
    20881 ccgacagctg ccttggctgc agcgccttgt cgaaagtgag gcccggcagg cggagatcgg
    20941 catggccgcg cccgctcgcc agatcatgcg ccagcgaaac ctgcgccagc gggcgatcgg
    21001 ccgccgtggc gctgaagctg gcggtggcga ccagctgccc gccggccagc gccagatcgg
    21061 ccagatgcag ccgcaccggc gcaaagcgcg gctgggccgc cccgccggtg gcggccgggt
    21121 cttccagcag cgcctcgccg cctgtcagcg ccagccgccc ggcggcccag cgcaaacggc
    21181 ccgatgccgc gcgcagggcc accggggcat cggctaggcg cgcctcgcca tcggcaaagc
    21241 ggccggtgat ctcggggcca aggcgcaggg ccagctggcc cagatggagc tggcccgcat
    21301 tggcgccgcc ccgctccacc gccacatcgt gcgcctccag cccgcccggc cagcccagcg
    21361 ccaaagcgcc ggccgccacc cgcagcgggg cgccctgcat ctcgcccgcc agcgccacgc
    21421 cgcccgttcg caccgacaca cgcatgccct gcggccccag cgccagcatc gccccgcccg
    21481 gccccgggca caggcgcagg gccttgccgc ccgccggcgc cagctccgcc tcgcccagcg
    21541 ccagccgctc cagccccagc gccaggcagc gccgccccag caccagcccg ctgcccgccg
    21601 accagcgccc ctcgatcggt atcgccagcc cggccacgct ggggccggcc gccggatcgc
    21661 ccgtcagcgg cccgccgaga atgacgcgcc cggccagctc caccgcgccg cccggcgcct
    21721 gccgcaaggc cagctgcggc accgccaggc tggccccgcc ggccgcccat gccggcatcg
    21781 ccagcaccgc ctcggcccgg ccatcggcgc tggtggccag ctgcccgcgc agcgccggca
    21841 gccccgtgcc ccccagcgcg aagcggcccg ccaccaccgg cgcgccccgc cccgccacca
    21901 ccgacacatg cgaggccgac agccaataag gcccgcccgc ttcatacacg cgcaattccg
    21961 gcaggatcag gctggtggtg ccggccgaac ggcgcagcgc ccaattgccc gccagccggc
    22021 tttgcccggc cgcccgcagc aggccatcgt ggaaggcgcc ggccagtggc gcggcccagc
    22081 tgcccgccgc cgccgcccgc cagctgccca gccgggccac cagctgagcc gaaggcgcca
    22141 gcccgctgcc gctgaggctg ccctcggccg ccagcgccgc gccgccacgg gtgaggcgca
    22201 catggcccgc cagccccaac tggcgcgcgc tggcggcaag gccggccacg ccctcgccgc
    22261 ccagggtgaa atcggccgcc agcacggcct ttccggcatt gggcccggca ttaggcccgg
    22321 cgggggccag atcgcgcagg ctgaaaccag cgctgccctc cagccgcaca gcctgcacac
    22381 cgcccccatg cgccggcccg gcatgcagca ccaaccgccc ggcgccgctc tccagcccgg
    22441 gccccagccc catcgccaca tccagcaccg gccgcgccac cgccagcccc gtgcccgcac
    22501 acgccagatc gcccgcctcc agccggcctg ccagctgcgg ctgcccggcg gcaacggcca
    22561 gctgcccggc aaaatgcccc ccgcgcgcca ggcagccctg cccgcgcaaa gccggcgcca
    22621 ctaccgccag ccgagcgcca aagccgtcac gca
//
LOCUS       DASRYE010000172        17895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_204416,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000172 DASRYE010000000
VERSION     DASRYE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 17895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17895
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204416"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VFF98_08955"
     CDS             complement(<1..1455)
                     /locus_tag="VFF98_08955"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HZU63800.1"
                     /translation="MMALWGNRDSGRASAGHASGEHAIIDIGSNTVRLVIYDGPPRAP
                     TVLYNEKVTARLGKGVADNGLLAEKGSALALSALGRFAAILRLRAVADVQCVATAAVR
                     DAGNGGAFLKDVAALGLNPRLLSGEEEALTSARGIISAFPGARGIVGDLGGGSLELVD
                     VADGTCTHGTSLPLGTLRLPGLRADGPARFTRRIDKMLRATEWIGQHDQPFYLVGGSF
                     RALARLAMARQEWPVDDPHGFEMAPDAALHLARSVASTAPAFAKPVAGRGAGKAAVGL
                     VPGFAVDPQLLGISAGRLASLPDAAALLGVLVRELKPSVLVFSGWGLREGLLAGRLDA
                     RTAASDPLLAGVGAFVEAQLPGIGETARAMAGWTARVLAPAPGPTPAPTPAPTPAGES
                     LRLATTMLALASMRTEPNLRAEQAAQWALRKRWIGLDAAGRAMLALGVLANSGRTAVL
                     PELERLAPPERLREALVWGLATRLARRFSALAPGA"
     gene            complement(1465..3675)
                     /locus_tag="VFF98_08960"
     CDS             complement(1465..3675)
                     /locus_tag="VFF98_08960"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HZU63801.1"
                     /translation="MTAYQKAATHLEASPEEAEPLPVGVDLPVDVPGRFFNRELSWLA
                     FNQRVLAEARNPAYPLLERLRFLSISGSNLDEFLMVRVAGLAGQVRRSIEEISVDGLS
                     PAQQLAVTRDEVVRLERKQQAIWTELRTELAGQGITLFGASPDGGVTLTPTQTAWLKD
                     YFQERILPVITPQAIDPAHPFPFIGNGGMGLMISLVRLSDGADVMEMVLIPAALPRYI
                     RIAGENACYISIEDMIWRHADLLFPGFQMLGHGVFRVLRDSDIEVEEDAEDLVRYFRT
                     AIQRRRRGQVIMLQLQGDFDPGAENLLRDRLGLDLAIIIKSAGPLSMTGLAAIVDENR
                     PELKFEPYSPRYPERVLEHDGDIFAAMREKDMVVHHPYETFEVVIDFLRQAAADPDVV
                     AIKQTLYRAGKQSAIIAALCQAAENGKSVTAVVELKARFDEEQNLHWAAQLERAGVQV
                     IYGFVDWKTHAKISMVVRREGDAYRTYCHFGTGNYHPITARIYTDLSFFTADPALGRD
                     AGQVFNFITGYVEPRRTSLLGVAPVSLRQRLYGSIDREIANARAGKPAQIWGKLNSLT
                     DPDLIDRLYAASRAGVEVSLVVRGICCLKPGVAGLSENIRVKSIIGRFLEHGRIWAFA
                     CGSRLPHPRARVFISSADGMSRNLDRRVEVLVPIRNKTVHDQVLDQVMMANLLDTEQS
                     WLLSPDGTYARAEPGAQPFNLHRYFMTNPSLSGRGAAAGQGRKVPKLALRRGRA"
     gene            complement(3776..4246)
                     /gene="queF"
                     /locus_tag="VFF98_08965"
     CDS             complement(3776..4246)
                     /gene="queF"
                     /locus_tag="VFF98_08965"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZU63802.1"
                     /translation="MSDPSAPPPRPLHLGQASSLPASPEEAVLDYVPNPRPQALFTVR
                     FAAPEFTSLCPVTGQPDFAHLVLDYAPGETIVESKSLKLFLGSFRNHAGFHEDVTVGI
                     GMRLFHEMQPRWLRIGGYWYPRGGIPIDVFWQSGPAPEGLWVPDQGVAPYRGRG"
     gene            4379..5236
                     /gene="sseA"
                     /locus_tag="VFF98_08970"
     CDS             4379..5236
                     /gene="sseA"
                     /locus_tag="VFF98_08970"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HZU63803.1"
                     /translation="MESLVTTQWLADELAAGDLRVIDASSHLAAAGRDALAEHEAGHI
                     PGALFMDLAELVDTASPVENTLPGAEKFASRMQGLGVGDGSRIVVYDDSDIHSAARAW
                     FMFKLFGAQNVAILDGGLAKWKAEGRPLEAGRKVLRCRHYTAWSDGARLRGKADMLAN
                     IASGREQVADARGTPRFAGTAPEPRPGMAAGHIPGAHNLPYSALYQADGTFRPASELR
                     AAFARTGIDLARPVIATCGSGMTACVLIFALHLLGKEDVALYDGSWAEWGADPATPKE
                     TGIPKPQNA"
     gene            5249..6472
                     /gene="metC"
                     /locus_tag="VFF98_08975"
     CDS             5249..6472
                     /gene="metC"
                     /locus_tag="VFF98_08975"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HZU63804.1"
                     /translation="MPKTPDSPGPNTQLVAAGRRPEWTGTASHPSAVVSPPVWRASTH
                     LYPDMAALRAETGQNADGRFYYGRRGAPTQWALADALTALEPGAEGTVLYPSGVAAIA
                     GVLLALLKPGDVLLMTDNAYEPSRALARGLLAEMGVETRWFDPTDLAALEAAICPRTR
                     AVLLESPGSLTMEVQDVPAIAALCRQHAIASVLDNTWASPLGFAALAHGVDISLMSLS
                     KHVGGHSDLMMGCATATPQWHMALRRRAQMLGYCAAPDDAALALRGLRSMGLRLERTT
                     ASALAVAGWLQGRAEVARVLCPMLPGSPGHDLWRRDFTGGCGLFSFVFRGGTAAARDA
                     FIDALNLFGIGYSWGGFESLATPVDPARIRTASPWPLAGMAQTDCFGVRLAIGLEDAA
                     DLIADIEQALAAWQG"
     gene            6544..7353
                     /gene="cobA"
                     /locus_tag="VFF98_08980"
     CDS             6544..7353
                     /gene="cobA"
                     /locus_tag="VFF98_08980"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZU63805.1"
                     /translation="MNAPSLHATGPESGPVPGPKSGKVWLVGAGPGDPDLLTLRAARL
                     LMSARLVVHDGLVDPRILAMAPAGARLISVAKRRSRHTMPQEEINALLVREALAGRDV
                     LRLKGGDPFIFGRGGEEQEACRAAGVAVEVVPGISAAIGAGAAAGLALTHRAAASTVS
                     FVSGQCKNLTEQNWAGLAGQGRTLVVYMGIACADTIADKLMADGLAPDTPLAVVEKAC
                     RPDMRVLRAPLAGLGALVAKEQVQSPAIIIIGAVTAQDPIELRKVALEAEQ"
     gene            7350..7646
                     /locus_tag="VFF98_08985"
     CDS             7350..7646
                     /locus_tag="VFF98_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HZU63806.1"
                     /translation="MKIVTGNDLKTGAVIWWTGEGWSLHVNEAAEAAEHAAEIIAREE
                     AARHVVGAYAIEATRDTEGLRPAHIKDRIRALGPTVRLDLSLKPADPDAGNWVI"
     gene            7646..9280
                     /locus_tag="VFF98_08990"
     CDS             7646..9280
                     /locus_tag="VFF98_08990"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZU63807.1"
                     /translation="MYAYDQYDQQMVDARVAEFRDQTRRRLDGSLSEEKFRPLRLQNG
                     LYLQLHAYMLRVAVPYGTLNSAQMHVLGDIADKYDRGYGHFTTRQNIQYNWIKLEDAP
                     DILADLAKVEMHAIQTSGNCIRNISSDQYAGATADELIDPRPYAELLRQWSSFHPEFL
                     ALPRKFKICVIASDTDRAAMKLHDIGIQIVKNAAGEIGAAFYVGGGMGRTPMIAPQIN
                     PFVPLDQLITYSEAALRVYNRYGRRDNKYKARIKILVHELGLEEYRRQVEAEFAHLLT
                     QGIEPPLAELARIQAHFADPAFEAGLSGDIDLSNADFARWVKANTAAHKQPGYAIATI
                     SLKPIGGIPGDASADQIREMARLAKVYSFDELRVTHAQNIVLPHVRKADLFALWQALV
                     AADLATANLDAITDIIACPGLDYCSLANARSIPVAQKIAERFAAAEKADAIGELKLKI
                     SGCINACGHHHAGHIGILGVDRKGTENYQLLLGGSEGADTSLGTITGPGFSEDGIVDA
                     VEKATDVYLAHKQGEERFLDTFRRIGMAPFKEAIYG"
     gene            9273..9707
                     /locus_tag="VFF98_08995"
     CDS             9273..9707
                     /locus_tag="VFF98_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZU63808.1"
                     /translation="MVEVQFRFRDDEVVADPAVTVDAFGEQTNAVAVRLEPGDDARDL
                     LPYLDRLKLVEVTFPAFTDGRGYSSARILREAGYAGELRAVGDIGVDQLSHLKRVGFD
                     AIAPEAELNAEDATRAFATWPEVYQATVVDGRQPIWALRHGR"
     gene            9709..10512
                     /locus_tag="VFF98_09000"
     CDS             9709..10512
                     /locus_tag="VFF98_09000"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZU63809.1"
                     /translation="MGEARRIDRIDTAPRFTAAEAEALNHRFADADTDTLLRAVLTQG
                     VAGRVAVVSSFGAESAVLLHLVARVNPATPVLFLDTGKHFAETLAYRDALVARLGLTG
                     LVNLTPDADLLAKRDENGLRWSFDPDGCCDIRKVQPLARALAGFDATITGRKGFQSAT
                     RAGLPRFELDTADAAGRLKVNPLASWRAEDLAAWFAAHDLPAHPLVAEGYPSIGCSPC
                     TSKVQPGEDPRSGRWRGWDKTECGIHAASPVPGTDGKPGDLPPDYEPAF"
     gene            complement(10522..11436)
                     /locus_tag="VFF98_09005"
     CDS             complement(10522..11436)
                     /locus_tag="VFF98_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZU63810.1"
                     /translation="MSAHPIALTGATGFVGQALLAEAAARGCAVKALARRPQPPRAGV
                     EWVAGDLADKAALARLVAGAGAVVHVAGVVNAADAAGFEAGNVAGTAALIEAARAAGC
                     QRFVFVSSLAARAPALSAYGASKARAEGLVAASGLDWTIVRPPSVYGPRDTENFELFK
                     SARLGVVPVPRGVCASQIYVEDLARLLLDLVPATASLGATYEPDDGRPGGWPMALFAR
                     AIGQAVGRRVWVPQLSPGLLRLAARIDVALRGGRAKLTPDRASYMSHTDWVARAAAHV
                     PPAIWRPRVGGAEGLAATVAWYRAQGWL"
     gene            complement(11433..12575)
                     /gene="proB"
                     /locus_tag="VFF98_09010"
     CDS             complement(11433..12575)
                     /gene="proB"
                     /locus_tag="VFF98_09010"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZU63811.1"
                     /translation="MTRTHSIATLADLASPAACPRLVIKVGSALLVDGGGARAPWLAG
                     LVAEIAAARARGQEVIVVSSGSIALGARKLGLEKGGRGSLADAQAAAAVGQIALSGLW
                     AQLLADHGLTAAQLLLTLEDLEDRRRYLNATATLSRLLDAGAVPVVNENDSVATEEIR
                     FGDNDRLAARVAQAAKASAVLLLSDVDGLYDRHPSEPGAQLLPVVKGITKEIRAMASP
                     TSGSGMGSGGMSSKLQAADIANLAGIALVIGNGTHAAPIARVVEQGVGTVFLPGRRAA
                     ARKAWLGGRLRLKGVLVVDAGAARALAQGASLLAAGITAVEGHFARGDAVAIRDGEGR
                     VLAHGLAEYAAGECAAILGRQSADQEALLGYAPRSAVVHRDQLVLV"
     gene            complement(12665..13717)
                     /gene="obgE"
                     /locus_tag="VFF98_09015"
     CDS             complement(12665..13717)
                     /gene="obgE"
                     /locus_tag="VFF98_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZU63812.1"
                     /translation="MHFLDQAKIFIRSGSGGPGAVAFRREKYVEYGGPDGGDGGKGGD
                     IIFEGVAGLNTLIDFRYTQHFKAQRGHGGAGKNRTGAGGNDLVIHVPVGTQILDDERE
                     TVLADITEAGQRIMLLRGGDGGRGNASYKSSTNRAPRQHGPGWPGDEMYVWLRLKLLA
                     DVGLVGLPNAGKSTFINQLANTNAKVGDYPFTTLRPQLGVVRHKAREFVLADIPGLIA
                     GAADGAGIGDRFLGHIERCRVLIHLIDIAGKEPVEALEIIEDELAAYGEGLDEKPRLI
                     ALNKIDQADAELAAAYAAELIEAGAERVFPISGATGAGMEALLDAVISYLPAATVTER
                     PEGEREELDDKPWSPL"
     gene            complement(13848..13923)
                     /locus_tag="VFF98_09020"
     tRNA            complement(13848..13923)
                     /locus_tag="VFF98_09020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13888..13890),aa:Ala,seq:cgc)
     gene            complement(14057..14776)
                     /locus_tag="VFF98_09025"
     CDS             complement(14057..14776)
                     /locus_tag="VFF98_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63813.1"
                     /translation="MRKLVWFGAAGAALALAACSAPPPPPPPPPPPAPVVLVIPPRPM
                     PPAGATASLAIPTVGTDGLHHTPLSGQAEGLKLWNLRSAFNVAALNCSGPQYAELTPA
                     YSAFLKKQARALTLANKAVDTEFRQRDGKAFLHQREAYLTQLYNFYAFPPTLPAFCDE
                     ALDISRDSAGIAAKDLPAFADRHLGHVSRLYEAFYAAYDRYRADLAAWEAKYGAAAAA
                     ASSAAVVPGPAVASAREASRP"
     gene            complement(14951..15709)
                     /locus_tag="VFF98_09030"
     CDS             complement(14951..15709)
                     /locus_tag="VFF98_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZU63814.1"
                     /translation="MAIWNDPQPSQTGLGAASWMNAPYGAAARDAGLRAYMLRIYNFM
                     ASGVLVSGIVAIAMASLLEQHNPLAMALLFSPLRWLVILAPFAFILAMSFGFMRMQNS
                     TLQALFWAFCAVMGVSLSTVLLVYTGSSVAATFFATTGAFAGLSLVGYTTKRNLSAMG
                     TFLTMGLFGLIIAMLINAFFPSPGLSMGISLIGVLIFAGLTAWDTQRLKGMYLQVAGT
                     PYAEKAAIMGALTLYLDFVNMFMFLLRFMGDQRR"
     gene            complement(15847..16665)
                     /gene="proC"
                     /locus_tag="VFF98_09035"
     CDS             complement(15847..16665)
                     /gene="proC"
                     /locus_tag="VFF98_09035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZU63815.1"
                     /translation="MSASGGWPQRVLLVGCGNMGGAMLAGWLRAGLAADRFTIVDPSL
                     PEAPAGVRLLRALPEGERFDAVLLGVKPQALDGVAPQLAPALDGATVLLSILAGVELA
                     SLAARFPGIGGYVRVMPNLAAAIGKSPMGLAASGLDAAARDGVQSLLAPLGTPEWVGE
                     ELFHAVTALAGSGPAFVYRFIDALAQGGVAVGLDPAQAQRLALATVEGASALAGASPF
                     SPAELARRVASPGGTTQAGLDVMDAGSAMLQLGEATLRAARDRSVEMAEAARAG"
     gene            complement(16667..17179)
                     /locus_tag="VFF98_09040"
     CDS             complement(16667..17179)
                     /locus_tag="VFF98_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HZU63816.1"
                     /translation="MVTRTDQNEFGRHEDSAPVEMLAALFEARGWPFEFVTDDEITGE
                     VQGSWANYQVRGIWRAEDNVLQLLCLPDIRVPDDKRPAMFEVLGLINEQLWLGHFDIW
                     SNGQVLLYRHGLMLGDDGLLSLGQAQTVVEVAVEECDRFYPAFQFILWGDKSPTEALA
                     SALVDAAGEA"
     gene            complement(17381..>17895)
                     /locus_tag="VFF98_09045"
     CDS             complement(17381..>17895)
                     /locus_tag="VFF98_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU63817.1"
                     /translation="PPAPSVAPPVAPVAAAPVARPVAPRPQPRPVAPPRPTSFADAFG
                     AWAGAPNVERVSGAVDIRKITPARPAPKAPPPPAHPSRIWVQVGVGRDTDRIAFDWHR
                     MVRDEPELLHGRKPSVTEWGRTNRLLLGPFDTEAAAHAFNDKLRKAGHDDAFVWISPA
                     GQAVDALDQR"
BASE COUNT         2994 a         6408 c         5874 g         2619 t
ORIGIN      
        1 cgcgcccggc gccagcgccg aaaaccgccg cgccagccgc gtcgccagcc cccacaccag
       61 cgcctcgcgc aggcgttcgg gcggggccag ccgttccagt tccggcagca ccgccgtgcg
      121 cccggaattg gccagcacgc ccagcgccag catcgcccgg ccggcggcat cgaggccaat
      181 ccagcgcttg cgcagcgccc attgggcggc ctgctcggcg cgcaggttgg gctctgtgcg
      241 catggaggcc agcgccagca tggtggtggc aaggcgcagc gattcgccag ccggggtggg
      301 ggcgggagtc ggggccgggg tggggcccgg agcaggcgcc agcacccgcg ctgtccaccc
      361 ggccatggcg cgggcggtct cgccgatgcc gggcaattgc gcctcgacaa aggcaccaac
      421 gccggccagc agcggatcag aggcggccgt gcgcgcatcc agccgcccgg ccagcagccc
      481 ctcgcgcagg ccccagcccg aaaacaccag cacgctgggc ttcagctcgc gcaccagcac
      541 gcccagcagg gcggccgcat caggcaggct ggccaggcgc ccggccgaaa tgcccagcag
      601 ctgcggatcg accgcaaagc ccggcaccag ccccaccgcc gccttgcccg cgcccctgcc
      661 cgccaccggc ttggcaaagg cgggcgcggt ggaggccacg ctgcgcgcca gatgcagcgc
      721 cgcatcgggc gccatctcga agccatgggg gtcgtcaacc ggccattcct gccgcgccat
      781 cgccagccgc gccagcgcgc ggaacgagcc gccgacgagg tagaagggct ggtcgtgctg
      841 gccgatccat tcggtggcgc gcaacatctt gtcgatccgc cgagtgaaac gcgccggccc
      901 atcggccctg aggcccggca gccgcagcgt gcccagcggc aggctggtcc catgggtgca
      961 agtgccatcg gccacatcca ccagttccag gctgcccccg cccagatcgc ccacaatgcc
     1021 gcgcgcgccg ggaaaggccg agatgatgcc gcgcgcgctg gtcagcgctt cttcctcgcc
     1081 ggaaagcagc cgcgggttga ggccaagggc cgccacatcc ttgaggaaag cgccgccatt
     1141 gccggcatcg cgcaccgccg ctgttgccac gcattgcaca tcggccaccg cgcgcaggcg
     1201 caaaatcgcg gcaaagcgcc ccagggccga aagcgctagc gcgctgccct tttcggccag
     1261 caggccatta tccgccacgc ccttgcccag gcgcgccgtc accttctcgt tatacagcac
     1321 cgtgggcgcg cggggcggcc catcatagat gaccagccgc accgtgttcg agccgatgtc
     1381 gataatcgca tgttcgccgc tggcgtgccc ggcgcttgcc cggccgctgt cccgattgcc
     1441 ccacaaggcc atcatcgccc cccgtcaggc ccggccgcgc cgcagcgcca gcttgggcac
     1501 cttgcgcccc tgcccggccg ccgccccccg cccggaaagc gaggggttgg tcatgaaata
     1561 gcggtgcagg ttgaacggct gggcgcccgg ctcggcgcgg gcataggtgc catcggggct
     1621 cagcagccag ctctgctcgg tatccagcaa attggccatc atcacctgat ccagcacctg
     1681 atcatgcacg gtcttgttgc ggatgggcac cagcacttcc acgcgccggt caaggttgcg
     1741 gctcatgcca tcggcgctgg aaatgaacac ccgcgcgcgc ggatggggca gccggctgcc
     1801 acaggcaaag gcccagatgc ggccatgctc cagaaagcgg ccgatgatcg atttcaccct
     1861 gatattctcc gaaagcccgg caacgcccgg cttcaggcag cagatgccgc gcaccaccag
     1921 gctcacctcc acgcccgccc ggctggcggc atagagccgg tcgataaggt cggggtccgt
     1981 cagcgaattg agcttgcccc agatctgcgc gggcttgccg gcgcgggcat tggcgatctc
     2041 gcggtcgatg ctgccataga ggcgctggcg cagcgacacc ggcgccaccc ccagaagcga
     2101 ggtgcgccgc ggctccacat agccggtgat gaagttgaac acctgcccgg catcgcgccc
     2161 cagcgccgga tcggcagtga agaaggacag atcggtgtag atgcgcgcgg tgatcgggtg
     2221 ataattgccg gtgccaaagt ggcaataggt gcgataggca tcgccctcgc ggcgcaccac
     2281 catgctgatc ttggcatgcg tcttccagtc gacaaagcca tagatcacct gcacgccggc
     2341 gcgttccagc tgggcggccc agtgcaggtt ctgctcctcg tcgaaacgcg ccttcagctc
     2401 caccacggcg gtcacggact tgccgttttc cgctgcctga cacagggcgg cgatgatcgc
     2461 gctttgcttg ccggcgcgat agagcgtctg cttgatcgcc accacatccg ggtctgccgc
     2521 ggcctggcgc aggaaatcga tcaccacctc gaaggtctcg taggggtggt gcaccaccat
     2581 gtccttctcg cgcatggcgg caaagatgtc gccatcatgc tccagcacgc gttcgggata
     2641 gcggggcgaa tagggctcga acttcagctc gggccggttt tcatcgacga tcgcggccag
     2701 cccggtcatc gacagcggcc cggccgactt gatgatgatc gccagatcca gccccagccg
     2761 gtcgcgcagc aggttttccg cgccggggtc gaaatcgccc tgcagctgca gcatgatcac
     2821 ctgcccgcgc cggcgccgct ggatcgccgt gcggaaatag cgcacgagat cctcggcgtc
     2881 ttcctccacc tcgatatcgc tgtcacgcag cacgcggaac acgccgtggc ccagcatctg
     2941 gaagccgggg aagagcagat ccgcatgccg ccagatcatg tcttctatgc tgatatagca
     3001 ggcattttcg ccggcaatgc ggatatagcg cggcagggct gcggggatca gcaccatctc
     3061 catcacatcc gcgccatcgc tcagccgcac cagcgagatc atcagcccca tgccgccatt
     3121 gccgatgaac gggaagggat gggccggatc gatcgcctgc ggggtgatca ccggcaggat
     3181 gcgctcctgg aaataatcct tcagccaggc ggtctgggtg ggggtcaggg tcacgccgcc
     3241 atccgggctg gcgccaaaca gggtgatgcc ctgcccggcc agttcggtgc gcagttcggt
     3301 ccagatcgcc tgctgcttgc gctccagccg caccacctcg tcgcgcgtca cggccagctg
     3361 ctgggcgggc gagaggccat cgacggagat ttcctcgatg ctgcggcgca cctgcccggc
     3421 aaggccggcc acgcgcacca tcaggaattc atcaaggttg ctgcccgaga tcgacaggaa
     3481 gcgcagccgc tccagcagcg gataggccgg gttgcgcgct tcggccagca cccgctggtt
     3541 gaaggccagc cacgacagtt cgcggttgaa gaagcggccg ggcacatcca ccggcagatc
     3601 cacgcccaca ggcagcggtt ctgcctcttc cgggcttgcc tcaagatgcg tggcggcctt
     3661 ctgatacgcg gtcatgggtg ttggtcctgc acaggcaaac gggcttggtt gcccggtgtt
     3721 tatcagcttt tatccgcagc cggcgcgcgc tggcaagcca ggcgccggcc cgccttcatc
     3781 cccggccacg atagggcgcc acgccctgat cgggcaccca cagcccctcg ggcgccgggc
     3841 ccgattgcca gaacacgtcg atcgggatgc cgccgcgcgg ataccagtag ccgccgatgc
     3901 gcagccagcg cggctgcatc tcgtggaaca ggcgcatgcc aatgcccacc gtcacatcct
     3961 cgtggaagcc ggcatggttg cgaaagctgc ccagaaacag cttcagcgac ttcgattcga
     4021 cgatggtttc gcccggcgca taatcaagca ccagatgggc aaaatcgggc tggccggtca
     4081 ccgggcacag gctggtgaat tcgggcgcgg caaagcgcac ggtgaacagc gcctggggcc
     4141 gcggattggg cacataatca agcaccgcct cctcgggcga ggcgggcagg ctgctggctt
     4201 gcccgaggtg aagcgggcgc ggcggggggg cagaagggtc gctcatggcc cacccatgcc
     4261 gccaatgccg cgcccgcaca agaggaggac gcacaccccc ttcgccatag agaccattgc
     4321 agagcgcgaa attgacgcct aatccttcgc agtcgcagcg aaggaggagc gcgcgcggat
     4381 ggaaagcctt gtgaccaccc agtggctggc cgatgagttg gcggcgggcg acctgcgcgt
     4441 catcgatgcc agcagccacc tagccgcagc cggccgcgat gcgctggccg agcatgaggc
     4501 aggccatatc cccggcgccc tgttcatgga tctggccgag ctggtcgaca ccgcctcgcc
     4561 ggtggaaaac acgctgccgg gcgcggaaaa attcgccagc cgcatgcagg gcctgggcgt
     4621 gggcgatggc agccgcatcg tcgtctacga cgacagcgac atccattcgg ccgcgcgcgc
     4681 ctggttcatg ttcaagctgt tcggcgcgca gaatgtggcg atcctcgatg gcggcctggc
     4741 caaatggaag gccgagggcc gcccgctgga agccggccgc aaggtgctgc gctgccgcca
     4801 ttacaccgcc tggtcggatg gcgccaggct gcgcggcaag gccgatatgc tggccaatat
     4861 cgccagcggg cgcgagcagg tggccgatgc ccgcggcacc ccccgctttg ccggcaccgc
     4921 gcccgagccg cgccccggca tggccgccgg ccatattccg ggcgcgcata acctgcccta
     4981 ttccgcgctc tatcaggccg atggcacctt tcgcccggcc agcgagctgc gcgccgcctt
     5041 cgcccgcaca gggatcgatc ttgcgcgccc cgtcatcgcc acctgcggca gcggcatgac
     5101 cgcctgcgtg ctgatctttg ccctgcatct gctcggcaag gaggatgtgg cgctgtatga
     5161 tggcagctgg gccgaatggg gcgcagaccc tgccaccccc aaggaaaccg gcattcccaa
     5221 gccgcagaac gcctgagccc ccttacccat gcccaaaacc cccgattcgc ccggccccaa
     5281 cacacagctt gtggccgccg gccgccgccc ggaatggacc ggcaccgcca gccacccttc
     5341 ggccgttgtc agcccgccgg tgtggcgcgc cagcacccac ctttaccccg acatggcggc
     5401 cctgcgcgcc gaaaccggcc agaacgccga tggccgcttc tattatggcc ggcgcggcgc
     5461 ccccacccaa tgggcgctgg ccgatgccct gaccgcgctg gagccggggg ccgaaggcac
     5521 ggtgctctat ccatcgggcg tggcggcgat tgccggcgtg ctgctggcgc tgctgaagcc
     5581 gggcgacgtg ctgctgatga ccgacaacgc ctatgagccc agccgcgcgc tggcccgcgg
     5641 cctgctggcc gaaatgggcg tggaaacccg ctggtttgac cccaccgacc tggccgcgct
     5701 ggaagctgcc atctgcccgc gcacccgcgc cgtgctgctg gaaagcccgg gcagcctcac
     5761 catggaagtg caggatgtgc cggcgatcgc ggccctgtgc cgccagcacg ccatcgcctc
     5821 ggtgctggat aacacctggg ccagcccgct gggctttgca gcgctggccc atggggtgga
     5881 tatctcgctg atgagcctgt cgaaacatgt cggcggccat tccgatctga tgatgggctg
     5941 cgccacggcc acgccgcaat ggcacatggc gctgcgccgg cgcgcgcaga tgctgggcta
     6001 ttgcgccgcg cccgatgatg ctgcgctggc cctgcggggc ctgcgcagca tgggcctcag
     6061 gctggagcgc accaccgcca gcgcccttgc cgttgccggc tggctgcagg ggcgcgcgga
     6121 agtggcccgc gtgctgtgcc cgatgctgcc cggatcgccg gggcatgatc tttggcggcg
     6181 cgatttcacc ggcggctgcg gcctgttcag cttcgtcttc cggggcggca cggcggccgc
     6241 gcgcgatgcc ttcatcgatg cgctcaacct gttcggcatc ggctacagct ggggcggctt
     6301 tgagagcctg gcaacgccgg tggaccctgc ccgcatccgc accgcctcgc cctggccgct
     6361 ggcgggcatg gcgcaaacgg attgctttgg cgtgcgcctg gcgatcggac tggaggatgc
     6421 agcagacctc atcgccgata tcgagcaggc gctggccgcc tggcagggct aacaagtttc
     6481 ccttcccccg ttcaaagcca ttctcgctcg catcttggcg cggccggcgc caagggctgt
     6541 ggcatgaacg ccccttccct tcatgccaca ggcccagagt ctggccccgt gccgggccca
     6601 aaatccggca aggtctggct ggtgggcgcc ggccccggcg accccgacct gttgaccctg
     6661 cgcgccgccc gcctgctgat gagcgcgcgg ctggtggtgc atgacgggct ggtggatccg
     6721 cgcattctgg cgatggcgcc ggccggcgcg cggctgatca gcgtggccaa gcgccgcagc
     6781 cgccacacca tgccgcagga agagatcaac gccctcctcg tgcgcgaggc gctggccggg
     6841 cgcgatgtgc tgcggctgaa ggggggcgat cccttcatct tcggccgcgg cggcgaggaa
     6901 caggaggcct gccgcgccgc cggcgtggcg gtggaagtgg tgccgggcat ttccgcggcg
     6961 attggcgctg gcgcggcggc cggccttgcc ctcacccacc gggcggcggc cagcacggtc
     7021 agcttcgtct cgggccagtg caagaacctt accgagcaga actgggcggg ccttgccggc
     7081 cagggccgca cgctggtcgt ctatatgggc atcgcctgcg ccgacaccat cgccgacaag
     7141 ctgatggccg atggccttgc ccccgacacg ccgctggccg tggtggaaaa ggcctgccgg
     7201 ccagacatgc gcgtgctgcg cgcaccgctg gccgggcttg gcgcactggt ggccaaagaa
     7261 caggtgcaaa gcccggcaat catcattatc ggcgcggtga cggcgcaaga cccgatcgaa
     7321 ttgcgcaaag tggcgctgga ggctgaacag tgaaaatcgt gacaggcaac gacctgaaga
     7381 caggcgcggt gatctggtgg accggcgagg gctggtcgct gcatgtgaac gaggcggcag
     7441 aagcggccga acacgccgcc gagatcatcg cccgcgagga agccgcgcgc catgtggtgg
     7501 gcgcctatgc catcgaggcc acgcgcgata cagaagggct gcgcccggcc catatcaagg
     7561 atcgcatccg cgcccttggc cccactgtcc gcctcgacct ctccctcaag cctgccgacc
     7621 ccgatgccgg caattgggtg atctgatgta cgcttacgac caatacgacc agcagatggt
     7681 cgacgcccgc gtcgccgaat tccgcgacca gacccgccgc cgcctcgatg gcagcctgtc
     7741 ggaagagaag ttccgcccgc tgcgcctgca gaacggcctc tacctgcagc tgcatgccta
     7801 tatgctgcgc gtggcggtgc cctatggcac gctcaattcc gcgcagatgc atgtgctggg
     7861 cgatatcgcc gacaagtatg accgcggcta tggccatttc accacccggc agaacatcca
     7921 gtacaactgg atcaagctgg aagacgcgcc cgacatcctc gccgacctcg cgaaggtgga
     7981 gatgcacgcc atccagacct cgggcaattg catccgcaac atcagctccg accagtatgc
     8041 cggcgccacc gccgacgagc tgatcgaccc gcgcccctat gccgagctgc tgcgccagtg
     8101 gtcctcgttc catccggaat tcctggccct gccgcgcaag ttcaagatct gcgtgatcgc
     8161 ctcggatacc gatcgcgcgg cgatgaagct gcatgatatc ggcatccaga tcgtgaagaa
     8221 tgccgccggc gagatcggcg ctgccttcta tgtgggcggc ggcatgggcc gcacgccgat
     8281 gatcgcgccg cagatcaacc ccttcgtgcc gctcgaccag ctgatcacct attcggaagc
     8341 ggccctgcgc gtctacaacc gctatggccg gcgcgacaac aagtacaagg cgcgcatcaa
     8401 gatcctggtg cacgagctgg ggctggagga ataccgccgc caggtggagg cggaattcgc
     8461 ccatctgctc acgcaaggca tcgagccgcc gctggccgag ctggcgcgca ttcaggccca
     8521 tttcgccgat cccgccttcg aggccggcct ttcgggcgat atcgacctgt cgaacgccga
     8581 ttttgctcgc tgggtgaagg ccaacaccgc cgcccacaag cagccgggct atgccatcgc
     8641 caccatcagc ctgaagccca tcggcggcat tccgggcgat gccagcgccg accagatccg
     8701 cgagatggcg cgccttgcca aggtctacag cttcgacgag ctgcgcgtga cccatgccca
     8761 gaacatcgtg ctgccgcatg tgcggaaggc ggatctgttc gccctgtggc aggcgctggt
     8821 ggcggccgat ctggccacgg ccaacctcga tgccatcacc gatatcatcg cctgcccggg
     8881 cctggattat tgcagcctgg ccaatgcccg ctcgatccct gtggcgcaga agatcgccga
     8941 gcgctttgcc gcggctgaaa aggccgatgc catcggcgag ctgaagctga agatctcggg
     9001 ctgcatcaat gcctgcgggc accaccatgc cggccacatc ggcatccttg gcgttgatcg
     9061 caagggcacc gagaattacc agctgctgct gggcggcagc gagggcgccg acacgtcgct
     9121 gggcaccatc accggccccg gcttcagcga ggatggcatt gtcgacgcgg tggagaaggc
     9181 caccgatgtc tatctggccc acaagcaggg cgaggaacgc ttcctcgaca ctttccgccg
     9241 cattggcatg gcccccttca aggaagcgat ctatggttga ggtgcagttc cgtttccgcg
     9301 acgacgaggt cgtggctgat cccgccgtca ccgtcgatgc ctttggcgag cagaccaatg
     9361 ccgtggccgt gcggctggag ccgggcgacg acgcccgcga cctgctgccc tatctcgacc
     9421 ggctgaagct ggtggaagtg accttccccg ccttcaccga cgggcgcggc tattcctccg
     9481 cccgcatcct gcgcgaggcg ggctatgccg gcgaattgcg cgcggtgggc gatatcgggg
     9541 tggatcagct ttcgcatctg aagcgggtgg gctttgatgc catcgcccct gaggcggagc
     9601 tgaacgccga ggatgccacc cgcgcctttg ccacctggcc cgaggtctat caggcaacgg
     9661 tggtggatgg ccgccagccg atctgggcgc tgaggcacgg gcgctgacat gggcgaagca
     9721 cgccgcatcg accggatcga cactgccccc cgcttcaccg ccgccgaggc cgaagcgctg
     9781 aaccatcgct ttgccgatgc cgacaccgac acgctgctgc gcgcggtgct gacacaaggc
     9841 gtggccgggc gggtggcggt ggtctccagc ttcggggcgg aaagtgcggt gctgctgcat
     9901 ctggtggcgc gggtaaaccc cgccaccccg gtgctgttcc tcgacaccgg caagcatttt
     9961 gccgaaacgc tggcctatcg cgatgcgctg gtggcgcgcc tgggcctgac cggcctggtg
    10021 aacctcacgc ccgatgccga tctgctggcc aagcgcgacg agaacggcct caggtggtcg
    10081 ttcgacccgg atggctgctg cgatatccgc aaggtgcagc cgctggcccg ggcgctggcc
    10141 ggtttcgatg ccaccatcac cgggcgcaag ggcttccagt ccgccacccg cgccggcctg
    10201 ccgcgcttcg agctggatac tgcggatgcc gccggccggc tcaaggtgaa cccgctggcc
    10261 agctggcgcg ccgaggatct ggccgcctgg tttgccgcgc atgatctgcc cgcgcaccca
    10321 ctggtggccg aaggctatcc ctcgatcggc tgctcgccct gcaccagcaa ggtgcagccc
    10381 ggcgaagacc cccgctcggg ccgctggcgc gggtgggaca agaccgaatg cggcatccac
    10441 gccgccagcc ccgtgcccgg caccgatggc aagccgggcg acctgccgcc cgattacgaa
    10501 cccgccttct aaccgtcgcg cctagagcca gccctgggcg cgataccagg ccaccgtggc
    10561 cgccagccct tctgccccgc ccacgcgcgg gcgccagatc gccggcggca catgggcagc
    10621 ggcgcgcgcc acccaatcgg tgtggctcat atagctggcg cggtcgggcg tcagcttcgc
    10681 ccggcccccg cgcagcgcca catcgatccg cgcggccagc cgcagcagcc cgggcgacaa
    10741 ttgcggcacc cacacccgcc ggcccaccgc ctggccaatc gccctggcaa acagcgccat
    10801 cggccagcca ccggggcggc cgtcatcggg ctcgtaagtg gcgccaaggc tggctgtggc
    10861 cggcaccaga tcgagcagca gccgggccag atcctccaca taaatctgcg aggcgcacac
    10921 cccgcgcggc accggcacca cccccagccg ggcggatttg aacagctcga aattctccgt
    10981 atcgcgcggg ccatagacag agggcgggcg cacgatcgtc cagtccagcc cgctggccgc
    11041 caccagccct tcggcccgcg ctttggatgc gccataggcc gacagcgccg gcgcgcgcgc
    11101 ggccagcgag gagacgaaga cgaagcgctg gcagcctgcc gcccgcgccg cctcgatcag
    11161 cgccgcggtg ccggccacat tgcccgcctc gaaaccggcc gcatcggctg cgttcaccac
    11221 gccggccaca tgcaccaccg cgcccgcgcc cgccaccagc cgcgccagcg ccgccttgtc
    11281 cgcaagatcg cctgccaccc attccacgcc cgcgcggggc ggctgcgggc gcctcgccag
    11341 cgccttcacc gcgcacccgc gcgccgccgc ttcggccagc agcgcctggc cgacaaagcc
    11401 agtcgcccct gtcagcgcga tggggtgcgc gctcacacca gcaccagctg atcgcgatgc
    11461 accaccgccg agcgcggggc atagcccagc agcgcttcct gatcggcgct ctgccggcca
    11521 aggatggcgg cgcattcgcc ggcggcatat tccgccagcc catgggccag cacgcgcccc
    11581 tcgccatcgc ggatggccac cgcatcgccg cgggcaaaat ggccttccac cgcggtgatg
    11641 ccggccgcca gcaggctggc cccctgcgcc agcgcccttg ccgcgcccgc atccaccacc
    11701 agaacgccct tcagccgcag ccgcccgccc agccaggcct tgcgcgccgc cgcccgccgg
    11761 ccgggcagga acaccgtgcc cacgccctgt tccaccacgc gggcgatggg cgctgcatgg
    11821 gtaccattgc cgatcaccag ggcaatgccg gcaagattgg cgatatcggc cgcctgcaac
    11881 ttggagctca tgccgccaga gcccatgccg ctgcccgagg tggggctggc catggcgcgg
    11941 atctccttgg tgatgccctt caccaccggc agcagctgcg cgccgggctc ggaagggtgg
    12001 cggtcataca ggccatcgac atccgacagc agcagcaccg cgctcgcctt ggcggcctgc
    12061 gccacgcggg cggccagccg gtcgttgtcg ccaaagcgga tctcctcggt ggcgaccgaa
    12121 tcattctcgt tgaccaccgg caccgcgccc gcatcgagca gccggctgag cgtggcggtg
    12181 gcgttgagat agcgacggcg gtcttccaga tcctccagcg tcagcagcag ctgcgcggcg
    12241 gtcagcccat ggtcggccag caattgcgcc cacaggcccg acagcgcgat ctgccccact
    12301 gccgctgccg cctgcgcatc ggccaggctc ccgcgcccgc ccttctccag ccccagcttg
    12361 cgcgcgccca gcgcgatgga gccggaagag accacgatca cttcctgccc gcgcgcccgg
    12421 gccgcggcga tctcggccac caggccggcc agccacggcg cgcgcgcgcc gccgccatcc
    12481 accagcaacg ccgagcccac cttgatgaca aggcgcgggc aagcagcggg cgaggcaaga
    12541 tcggcaaggg tggcgatgga atgggtgcgc gtcatccccg gcgcttaggg gaaaggggcg
    12601 gaaatgggaa gattgatgcg ccgcttatgc gccctgcctt cccttccccc tcacaacggc
    12661 ggccttacaa cggagaccag ggcttgtcgt ccagttcctc gcgctcgccc tcggggcgtt
    12721 cggtcacggt ggcggcgggc agataggaga tcaccgcatc gagcagcgcc tccatccccg
    12781 cgccggtggc gccagagatc gggaacaccc gctctgcgcc cgcctcgatc agctctgcgg
    12841 cataggcggc ggccagctcg gcatcggcct ggtcgatctt gttcagcgcg atgaggcgcg
    12901 gcttttcatc cagcccctcg ccataggcgg ccagttcgtc ctcgatgatc tccagcgctt
    12961 ccaccggctc cttgccggca atgtcgatca ggtggatcag cacccggcag cgctcgatat
    13021 ggccgaggaa gcggtcgcca atgccggcgc catcggcagc cccggcgatc aggccgggaa
    13081 tatcggccag cacgaattca cgcgccttgt ggcgcaccac gcccagctgc gggcgcaagg
    13141 tggtgaaggg ataatcgccc accttggcgt tggtgttggc cagctggttg atgaaggtgc
    13201 tcttgccggc attgggcagg cccaccagcc ccacatcggc cagcagcttc agccgcagcc
    13261 acacatacat ctcatcgccc ggccagcccg gcccgtgctg gcgcggcgcc cggttggtgg
    13321 aggatttgta gctggcattg ccgcgcccgc catcgccgcc gcgcagcagc atgatgcgct
    13381 ggccagcctc ggtgatgtcg gccagcaccg tctcgcgctc gtcgtcgagg atctgggtgc
    13441 ccaccggcac gtggatgacc agatcattgc cgccggcgcc cgtgcggttc ttgccggcgc
    13501 cgccatggcc gcgctgggcc ttgaaatgct gcgtgtagcg aaaatcgatc agcgtgttga
    13561 ggccggccac gccctcgaag atgatatcgc cgcccttgcc gccatcgccg ccatcggggc
    13621 caccatattc gacgtatttc tcgcgccgga aggcaaccgc ccccggcccg ccactgcccg
    13681 aacggatgaa aatcttggcc tgatcgagga aatgcatggg gtatcgtcca accgcggcga
    13741 gggcttcaac atggccccgc cggcgccgcc gcaccccaag gccggtaatg cgaaaaaggc
    13801 cgccggccct gccgacagcc cccaacgctt gatctcaaca tcgcatctgg tggagccgag
    13861 ggggatcgaa cccctgacct cgtcattgcg aacgacgcgc tctcccagct gagctacggc
    13921 cccgttccag tgcgagtgcg gcttggcggg cgaacccgcc gccgcaagag gtgtgcccat
    13981 tagcgaagcc atcgccccat gggaagagga aaaatcgcca tcatgcgaat tctcctctcg
    14041 ccctgggcag atgggctcag ggccggctgg cctcgcgggc actggccacg gcagggccgg
    14101 gcaccacagc cgcgctggaa gcagccgcag ccgccgcgcc atacttcgcc tcccaggcgg
    14161 ccagatcggc gcgatagcgg tcgtaggcgg catagaacgc ctcatacagc cggctgacat
    14221 ggcccagatg gcgatcggca aaggcgggca ggtccttggc ggcaatgccg gcgctatcgc
    14281 gcgagatatc cagcgcctcg tcgcagaagg ccggcagcgt gggcgggaaa gcgtagaaat
    14341 tgtaaagctg ggtcagatag gcctcgcgct gatgcaggaa ggccttgcca tcacgctggc
    14401 gaaactcggt atccaccgcc ttgttggcca gggtcagcgc gcgcgcctgc ttcttgagga
    14461 aggccgaata ggccggcgtc agctcggcat attgcgggcc actgcagttg agcgcggcca
    14521 cattgaaggc cgaacgcagg ttccacagct tcagcccttc cgcctggcca gacagcgggg
    14581 tgtgatgcag gccatcggtg cccaccgtgg ggatggcaag gctggcggtg gcgccggccg
    14641 gcggcatcgg ccggggcggg atcaccagca ccaccggggc gggcggcggc ggtgggggcg
    14701 gcgggggcgg cggcgcggaa caggccgcca gggccagcgc cgcgccggcc gcaccaaacc
    14761 acaccaactt gcgcatgggc tgcatctgtc ttcaatctcc attgtcgcaa ggcctgtctt
    14821 atcggccctg cccatttacg atgatgcccc tagacgagcc ccttgccaaa caaaatgccc
    14881 gaggcgccgc aaggcacccc gggcacgatg aaatgcgtgt cgcctgcgcc gaagcgccgg
    14941 cgccggccga tcagcggcgc tgatcgccca tgaagcgcag gaggaacatg aacatgttca
    15001 caaagtcgag atagagcgtc agcgcgccca tgatcgccgc cttctccgca taaggcgtgc
    15061 ccgccacctg cagatacatg cccttgaggc gctgcgtgtc ccaggcggtg aggccggcaa
    15121 agatcagcac gccgatcagc gagatgccca tgctgaggcc cggcgagggg aagaaagcgt
    15181 tgatcagcat ggcgatgatc aggccgaaca ggcccatggt gaggaacgtg cccatcgccg
    15241 agaggttgcg cttggtggtg tagcccacca ggctgaggcc ggcaaaggca ccggtggtgg
    15301 caaagaaggt ggccgccacc gagctgcccg tatagaccag aagaacggtg gagagcgaaa
    15361 cgcccatcac cgcacaaaag gcccagaaca gcgcctgcag cgtgctgttc tgcatccgca
    15421 tgaagccaaa gctcatggcg aggatgaacg cgaagggcgc cagaatcacc agccagcgca
    15481 gcgggctgaa cagcagcgcc atcgccagcg gattgtgctg ttccagcagg ctggccatgg
    15541 cgatggccac aatgcccgac accagcacgc cagaagccat gaaattgtag atccgcaaca
    15601 tgtaggcgcg caggcccgca tcgcgcgcgg ccgcgccata gggcgcattc atccaagacg
    15661 ccgcgccaag gcccgtctgt gagggctggg ggtcattcca gatggccatc gataatcact
    15721 ccgtttcccg ggcggcgcaa aaggcgcaac ccggctggaa tcattattgg cgcagcggcc
    15781 gccgctttca agggaaagcg gcacaaaatg cgatggaatg cccggccgga tgcatccggc
    15841 ccgccttcaa ccggcgcgcg cggcctcggc catttccacg ctgcgatcgc gggcggcgcg
    15901 caaggtggcc tcgcccagtt gcagcatggc gctgccggca tccatcacat ccagcccggc
    15961 ctgggtggtg ccgccggggc tggccacgcg ccgggccagc tcggccgggc tgaagggcga
    16021 ggcccctgcc agcgccgagg ccccttccac cgtggccagc gccaggcgct gcgcctgcgc
    16081 cggatcgagg ccgacggcca cgcccccttg cgccagcgca tcgataaagc ggtagacgaa
    16141 agccgggccg gagccggcca gcgcggtgac cgcatggaac agctcctcgc ccacccattc
    16201 cggcgtgccc agcggcgcca gcagcgactg cacgccatcg cgcgcggccg cgtccagccc
    16261 gctggcggcc aggcccatcg ggcttttgcc gatggcagcg gccagattgg gcatcacccg
    16321 cacatagccg ccaatgccgg gaaaacgcgc ggcaaggctg gccagttcca cccccgccag
    16381 aatcgacagc agcaccgtgg cgccatccag cgccggcgcc agctggggcg ccacgccatc
    16441 cagcgcctgc ggcttcacgc ccagcagcac ggcatcgaag cgctccccct cgggcagggc
    16501 gcgcagcagg cgcacgccgg ccggcgcttc gggcagcgag ggatcgacga tggtgaagcg
    16561 atcggcggca aggcccgcgc gcagccagcc ggccagcatc gccccgccca tattgccgca
    16621 gcccaccagc agcacgcgct gcggccatcc gccagaagcg ctcatctcag gcctcccccg
    16681 ccgcatcgac cagcgcgctg gccagcgcct cggtcgggct cttgtcgccc cagaggatga
    16741 actggaaggc cgggtagaag cggtcgcatt cctccaccgc cacttccacc accgtctgcg
    16801 cctggcccag gctcagcagc ccgtcatcgc ccagcatcag cccgtggcga tagagcagca
    16861 cctgcccgtt cgaccagatg tcgaaatggc ccagccacag ctgctcgttg atcagcccca
    16921 gcacctcgaa catcgccggc cgcttgtcgt cgggcacgcg gatatcgggc aggcacagca
    16981 attgcagcac gttatcttcc gcccgccaga tgccgcgcac ctgataattg gcccagctgc
    17041 cctgcacttc gccggtgatc tcgtcgtcgg tcacaaattc gaacggccag ccgcgcgcct
    17101 cgaacagagc cgccaacatc tctaccggcg cactgtcctc gtgccggccg aattcattct
    17161 gatccgttct cgttaccatt gaatcgacct cagacacttt actctggcgc tgcgcaccgg
    17221 gttgccggca aagatggtct gcctcgccga tattgcacaa tggaaaccta gcgctggctt
    17281 gagcataact ccataagcct gtgcacatct tgtggacaat gtccgtttcc gggacaaatt
    17341 attcagccac aatcaatatc cagcccattt gggctgcatt tcaacgttga tccagcgcat
    17401 ccaccgcctg ccccgccggg ctgatccaca cgaaggcatc gtcatgcccg gccttgcgca
    17461 gcttgtcgtt gaaggcatgg gcggccgcct cggtgtcgaa cggccccagc agcaggcggt
    17521 tggtgcggcc ccattcggtc acggaaggct tgcggccatg cagcagctcg ggctcgtcgc
    17581 gcaccatgcg gtgccagtcg aaggcgatgc ggtcggtatc gcggcccacg cccacctgca
    17641 cccagatgcg gctggggtgc gcgggcggcg gcggcgcctt gggcgcaggc cgcgccggcg
    17701 tgatcttgcg gatatccacc gcgccagaca cccgctcgac attgggggcg ccggcccagg
    17761 cgccaaaggc atcggcaaag ctggtggggc gcggcggggc aacagggcgc ggctgcgggc
    17821 gcggcgccac cggccgggcc acgggcgctg cggcaaccgg agcgaccggc ggagcaaccg
    17881 acggggccgg cgggg
//
LOCUS       DASRYE010000173         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_204460,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000173 DASRYE010000000
VERSION     DASRYE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204460"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFF98_09050"
     CDS             <1..506
                     /locus_tag="VFF98_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63818.1"
                     /translation="GERPALNLDIPSGPGLPIDAAAMADARIDAAAMAKEARKGAPAP
                     FASLPPARQDDRLRALYRQGFHKAPDFAGLAPGAAAPAAAGAPADSAAKPARQLAEVA
                     WLRGQLRSAYAPSAQDLAQLGQARGAAIRDALAGKGAIDPARLFLSTTAPLAEKDGAI
                     RFELKLH"
     gene            571..1143
                     /locus_tag="VFF98_09055"
     CDS             571..1143
                     /locus_tag="VFF98_09055"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU63819.1"
                     /translation="MPRLVDREERRQMVMAVAFDLVARIGIEELTFKQVAAATGYSTA
                     IVSHYFQNKNELLFKVYELANGRARARLMAAFEADLPLIDCFEQILPIGEDSQKNWRV
                     WLAFWGRVHLDPAYAEQRRLASVDSLALYRAMLAERAPALHGAALDEAAERLLVAVAG
                     TALEACFNPAHWSARRQRAIIAAELARLPT"
     gene            1274..1906
                     /locus_tag="VFF98_09060"
     CDS             1274..1906
                     /locus_tag="VFF98_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZU63820.1"
                     /translation="MIRHVVMIKFKKDAPQDKIDLFLTELKKLPHQNKEIRDYSHGLC
                     VKTRYHSGDFDFANCCDIDSYEAMERYMSHWAHLRMTPYLPDILENLLSFDWEIDYHG
                     PAFDEAAAKEEAVKEAARVLPPHADPAKAFVPEIRGQTRERAKEMLDTVGLVLAPEEE
                     EIIGSCWAPNRIMFTSPDVGSEVDKGTVVKIGITGDWLTGPAIPGGQPAW"
     gene            1987..3168
                     /locus_tag="VFF98_09065"
     CDS             1987..3168
                     /locus_tag="VFF98_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU63821.1"
                     /translation="MTTLEDTYGDIAEKLLHFVESRSTDQAGAVMQVPAGDYLDPALW
                     QRELDMIFRRLPLAMALTIEMPAPGDYKAMEVMGLPLLLTRGRDGKARAFFNVCKHRA
                     MQLVEPGRGNCKGFACPYHGWTYGVDGKLVAIAEASTFGDVDKATLGLTELPCEEVAG
                     LIFAILTPGLAIDVPAFLGGMLEDLAALRLETWYFHKSRPLPGANWKIAYDGYLEGYH
                     FQAAHPETVTPRTPSNRAWYDAFGPHIRLAYPQHRITELRDLPRDEWGRRENLNYDFV
                     RMLFPNFAMFSAPEMCQVAQLFPGKDVESNITLMNYIFPKQPESAEELARLDEMCDFF
                     YKVVEEEDYLMNFRVQRGLKAGASETVNFGRNEPGNQLFHHYLAYYLGGASTPAPQLR
                     G"
     gene            3192..>3280
                     /locus_tag="VFF98_09070"
     CDS             3192..>3280
                     /locus_tag="VFF98_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HZU63822.1"
                     /translation="MPHPLSGKTALVTGGSRGIGRAVALRLAA"
BASE COUNT          561 a         1144 c         1047 g          528 t
ORIGIN      
        1 tcggcgagcg gcccgcgctc aacctcgaca tcccctccgg ccccggcctg ccgatcgatg
       61 ccgccgccat ggccgatgcc cgcatcgatg ccgccgccat ggcgaaggaa gcccgcaagg
      121 gcgccccggc ccccttcgcc agcctgccgc ccgcccggca ggatgatcgc ctgcgcgcgc
      181 tttacaggca gggcttccac aaggcgcccg attttgccgg cctggcgccc ggcgccgccg
      241 cccctgccgc tgctggagcc cctgccgaca gcgccgccaa gcccgcccgc cagctggccg
      301 aagtggcctg gctgcgcggc cagctgcgca gcgcctatgc cccctcggcg caggatctgg
      361 cgcaactggg ccaggcgcgc ggcgctgcca tccgcgatgc gctggccggc aagggcgcga
      421 tcgatccggc ccgcctgttc ctgtccacca ccgcgccgct ggccgagaag gacggcgcca
      481 tccgtttcga gctgaaactg cactaatgcc agcttgcgcc agcggccggc cgcgcatagc
      541 ccaagccgcc tgcgcaggaa ggagacgccg atgccgcgcc tggtagaccg ggaagagcgc
      601 cgccagatgg tgatggccgt ggccttcgac ctggtcgccc ggatcggcat agaggagctg
      661 actttcaagc aggtggcggc ggccaccggc tattccacgg cgatcgtctc gcattatttc
      721 cagaacaaga acgagctgct gttcaaggtc tacgagctgg ccaatggccg cgcccgggcc
      781 cggctgatgg ccgctttcga ggccgacctg ccgctgatcg actgtttcga gcagatcctg
      841 ccgatcggcg aggacagcca gaagaactgg cgtgtgtggc tggccttctg gggccgggtg
      901 catctcgatc cggcctatgc cgagcagcgc cgcctcgcct cggtagacag ccttgcgctg
      961 tatcgcgcca tgctggcaga acgggcgccg gcgctgcatg gcgcagcgct cgacgaggcg
     1021 gccgagcgct tgctggtggc ggtggccggc accgcgctgg aggcctgctt caacccggcg
     1081 cactggtctg cccggcgcca gcgggcgatc atcgcggcgg aactggcccg cctgcccacc
     1141 tgaggcaagc tggcctgagg cacgcgacct gttttttctg acagtcatca cataaccgct
     1201 tctgtgcgac tgtgcctccc gacagcgcca cgcccacgcc aggccaaccg gcgcgcaacc
     1261 gggagatgcg acgatgattc gccatgtggt gatgatcaag ttcaagaagg atgccccgca
     1321 ggacaagatc gacctgttcc tgaccgagct gaagaagctg ccgcatcaga acaaggaaat
     1381 ccgcgactac agccacggcc tgtgcgtgaa gacgcgctat cactcgggcg atttcgattt
     1441 cgccaattgc tgcgacatcg acagctacga ggcgatggag cgctacatgt cgcactgggc
     1501 acatctgcgc atgacgccgt acctgcccga tatcctcgaa aacctgctct ccttcgactg
     1561 ggagatcgac taccacggcc ccgccttcga cgaggcggcg gcaaaggaag aggcggtgaa
     1621 ggaagcggcc cgcgtgctgc ccccccatgc cgatccggcc aaggccttcg tgcccgaaat
     1681 ccgcgggcag acgcgggaac gggcgaagga aatgctggac accgtgggcc tggtgctggc
     1741 ccccgaggaa gaggaaatca tcggctcctg ctgggcgccc aaccgcatca tgttcaccag
     1801 ccccgatgtg ggcagcgagg tggacaaggg caccgtcgtc aagatcggca tcaccggcga
     1861 ctggctgacc ggccctgcca tccccggcgg ccagccggcc tggtaacgcg cttctccccc
     1921 ctgccggcgg ccgcgccttc gggccggccg cctccttttt gccctcctgc cggcaaggtg
     1981 aagcccatga ccacgcttga agacacctat ggcgatatcg ccgaaaagct gctccatttc
     2041 gtggaaagcc gcagcaccga tcaggccggc gccgtgatgc aggtgccggc gggcgattat
     2101 ctcgacccgg ccctgtggca gcgcgagctc gacatgatct tccgccgcct gccgctggcc
     2161 atggcgctga cgatcgagat gcccgcccct ggcgattaca aggcgatgga agtgatgggc
     2221 ctgcccctcc tcctcacccg cggcagggat ggcaaggcgc gcgccttctt caacgtgtgc
     2281 aagcaccggg cgatgcagct ggtggaaccg ggccggggca actgcaaggg ctttgcctgc
     2341 ccctatcacg gctggaccta cggcgtggat ggcaagctgg tggcgattgc cgaggcgagc
     2401 acctttggcg atgtggacaa ggccacgctg ggcctgaccg aactgccctg cgaggaagtg
     2461 gccggcctta tctttgccat cctcacccct ggccttgcca tcgatgtgcc ggccttcctg
     2521 ggcggcatgc tggaggatct ggccgcgctg cggctggaga catggtattt ccacaagagc
     2581 cgcccgctgc ccggcgccaa ctggaagatc gcctatgacg gctatctcga aggctatcac
     2641 ttccaggccg cccaccccga aacggtgacc ccgcgcacgc cttccaaccg cgcctggtat
     2701 gatgccttcg ggccgcatat ccgcctggcc tatccgcagc accgcatcac cgaactgcgc
     2761 gatctgccgc gcgatgaatg gggccggcgc gagaacctca actatgattt cgtgcgcatg
     2821 ctgttcccca atttcgccat gttttccgcg ccagagatgt gccaggtggc gcaattgttc
     2881 cccggcaagg atgtggaaag caatatcacg ctgatgaact atatcttccc caagcagccg
     2941 gaaagcgcag aagaactcgc ccgcctcgac gagatgtgcg acttcttcta caaggtggtg
     3001 gaggaagagg attacctgat gaacttccgc gtgcagcgcg gcctgaaggc gggcgccagc
     3061 gagacggtca actttggccg caacgagccg ggcaaccagc tgttccacca ttatctcgcc
     3121 tattacctgg gcggcgccag cacgcccgcg ccgcagctgc gcggctgacc tgcccgttga
     3181 aggagacaag catgccccat cccctttccg gcaagacagc gcttgtcacc ggcggttcgc
     3241 gcggcattgg ccgggccgtg gccctgcggc tggcggccga
//
LOCUS       DASRYE010000174         9387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_41082,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000174 DASRYE010000000
VERSION     DASRYE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41082"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFF98_09075"
     CDS             complement(<1..427)
                     /locus_tag="VFF98_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZU63823.1"
                     /translation="MRAALWAIFRRDLAVLLLGGRQGGALLPVLFFLAVAVVFPFAVG
                     PDAALLARCGGGVLWVAALLAAILPIDRLVEPDLDQGFFDQWALRGLAEEGVVAVRIA
                     AHWLSFAPPLLLAALPAGALLGLSGEAIAMVELGLLAGTP"
     gene            complement(424..1041)
                     /gene="ccmA"
                     /locus_tag="VFF98_09080"
     CDS             complement(424..1041)
                     /gene="ccmA"
                     /locus_tag="VFF98_09080"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZU63824.1"
                     /translation="MQASPLLLSARALACRRADRMLFAGLDLALAPGAALHLAGANGI
                     GKSSLMRILAGLAAPFAGTVERHGPVALVQEKLALDGSQPLGRALAFWAGIDGADPGP
                     HAARLGLADLLDVPVRYLSTGQRKRAALARLLGQQAAIWLLDEPLNGLDTQGCALVGA
                     LVAEHIGTGGACLIASHQPFALPAGALPRLELAGFVPAPESGEWA"
     gene            1102..1404
                     /locus_tag="VFF98_09085"
     CDS             1102..1404
                     /locus_tag="VFF98_09085"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZU63825.1"
                     /translation="MPACPPLLTAPECAEALAALPLWRLADEGRAIARDLVFADFAAA
                     FAFMAHVALLAERADHHPDWRNVWNRVEIGLTTHAAGGLTRRDIALAQAIDRVAGG"
     gene            complement(1913..2530)
                     /locus_tag="VFF98_09090"
     CDS             complement(1913..2530)
                     /locus_tag="VFF98_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZU63826.1"
                     /translation="MGLEAETIRTGLDALAMREPAVARALAVVGYPAPRIRARGLATL
                     LRTIVGQQVSVAAAASVWARLEAALGAGVPAQALLAADDEALRACGLSRQKAGYARSL
                     CALVADGTLDLAALPAEDEAAIAALTAAKGIGRWSAEIYLLFAEGRADIWPAGDLGVQ
                     AGLHKLLALPERPREAPTRAMAESWRPHRGAMALFTWHCYDNPAL"
     gene            2665..2982
                     /locus_tag="VFF98_09095"
     CDS             2665..2982
                     /locus_tag="VFF98_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU63827.1"
                     /translation="MPKLVVVNRAGEETTVDAEVGRTVMEVVRDNGFDEMLALCGGCC
                     SCATCHVHVDPAFADKLPAITADEDDLLDSSDHRDGNSRLACQLPITEALEGLKITIA
                     QED"
     gene            3103..4110
                     /locus_tag="VFF98_09100"
     CDS             3103..4110
                     /locus_tag="VFF98_09100"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZU63828.1"
                     /translation="MTVAPVSSDTLGLIGNTPLVLLQGPSEAAGCEIWGKCEFANPGQ
                     SVKDRAALWIVRDAEKRGLLKPGGTIVEGTAGNTGIGLALVANALGYRTIIVMPETQS
                     REKMDTLRALRAELVLVPAAPFSNPGHFVHTSRRLAEETEGAIWANQFDNIANRRAHI
                     ESTAPEIWAQMEGRITGFTCAAGTGGTLAGVGLGLKAFDENVVIALTDPHGAALYNYY
                     AHGELKAEGSSVAEGIGQGRITANLDGAPVDTQFRIADAEGLHWVERLLAEEGLALGL
                     SSGINVAGAVALGRQLVAEGREDVRIATILCDTGFRYLSTLYNRAWLEAKGLPVFPWL
                     A"
     gene            4128..4460
                     /locus_tag="VFF98_09105"
     CDS             4128..4460
                     /locus_tag="VFF98_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63829.1"
                     /translation="MSKAPRPSALSAEPTYAGAPTARELRAQAVQRLQVGLFGLALML
                     LLVGLANIIMDHARQNETAAGAAIAVSSASSASGGSTDPLADIGVVPSPDADPAAHRP
                     TSRTAPTR"
     gene            4927..5424
                     /locus_tag="VFF98_09110"
     CDS             4927..5424
                     /locus_tag="VFF98_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZU63830.1"
                     /translation="MTGQPVIYSGQGFSPKGDKERFVLPAGFRRAVSESSAGANVLLV
                     SKHSSWPCLIGYGLSRRDSLIQQIDREEEMAARAGQPFDRDMRYFQLFDCAEISFDGS
                     GRFIIPAHLRKLVGIGEAIYFQGSGPFFTLWAPDVLAAQEGPLWASAQAACASLLEEP
                     GRGRK"
     gene            5421..6410
                     /gene="rsmH"
                     /locus_tag="VFF98_09115"
     CDS             5421..6410
                     /gene="rsmH"
                     /locus_tag="VFF98_09115"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZU63831.1"
                     /translation="MSLSGPYDLLPADAPHMPVLLAEVVAALAPAPGAVIVDATFGAG
                     GYTRALLAAGATVHAFDRDPDAIAAARLWPETQGDAPRLVLHHRPFSELAAGLAEAGV
                     AAIDAVVADIGVSSMQLDQAGRGFAFSADGPLDMRMAQEGPSAADFLNTASEEAIADV
                     LFHLGEERQSRRVARAIVAARPLATTGDLARVVRKALGHKPGAPKDPATRSFQAIRIH
                     VNDELGELNALLDAATGLLAPGGRLAVVSFHSLEDRIVKQFLREASGALPAASRHLPM
                     AAAGPAPLYERVSKAIRAGEAELAANPRARSATLRHAIRTTAPAPSDAHRRAA"
     gene            6407..6892
                     /locus_tag="VFF98_09120"
     CDS             6407..6892
                     /locus_tag="VFF98_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63832.1"
                     /translation="MNMTRDRLHAIGWGMTLTVCLALLVALTMRVNAVKSEVHLADRQ
                     IATLQQEKMFLETEFETRSSQQQLRAFNDVDFGYEAPTAGQYLESERQLAALGKPAGP
                     DAPAPVRMASADDGAGQGAGDAQPVRALVALAAEKDAQPHKLADRHPSRPARHSEAVE
                     E"
     gene            6889..8625
                     /locus_tag="VFF98_09125"
     CDS             6889..8625
                     /locus_tag="VFF98_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZU63833.1"
                     /translation="MTGIAGPQISAVISSVRSDSVVHRQRALAMARFRLLWVLIGFAA
                     LAFIALARLTQLGFAAGDRGNGAAGMAVDRGDIVDRNGVPLARAFPAYSLWFNPNALH
                     GGPALVKSPREVAEALVRIFPDENVDDLTRRLAAGKPGYLKRRILPEDANRIHALGEP
                     ALEFPRENERYYPQGSMAAHVLGYVDSDGHGQVGMEKVFDHQLTDPVTRGNPLVLSID
                     ARVQGVLEDELAKGLARAQAKSAGGIIMDVETGEVLALASLPSFNPNLIDRQGSEHAF
                     NLVVSQPYELGSVIKPITVATAIEAGVITSMARRYPAGKPLSIAGFEIHDDENFGDSL
                     NIPEALIHSSNIVLAQVGDQLGATRLRTAMEKLGFAERPYIELPARGLPRFPHADHGE
                     WSRVTTMTASFGHGFAITPLHLACAYAALVNGGIWHPATLQKLAPGQAPAGRRVFSAA
                     TSARMRQLLRVIVEVGTGRSANAPGFRVGGKTGTAERSDRFGYHRDQVVATFASAFPM
                     DHPRYVVIAMLDTPQANAATSYQRTAAWNSAPIVGRVVPRVGPLLGIMPDQTRDIDIS
                     DLKPLIANGDGE"
     gene            8622..>9387
                     /locus_tag="VFF98_09130"
     CDS             8622..>9387
                     /locus_tag="VFF98_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZU63834.1"
                     /translation="MKLAALLAEAGLAPVAGAEAAAVSGFAIDNRKVAPGNVFGAFAG
                     AVVNGEDFIPAAVAAGAVAVVARPQARVTGALHIAADNPRRAFARLAAGFYRPVPATI
                     VAVTGTNGKTSTVEMLRQIWRMAGQRAASIGTLGITTPEETTYTGLTTPDIVTFMANM
                     AGLAREGVSHVAYEASSHGLAQYRNEGLPVAAGGFTNLSRDHLDYHATMEAYFAAKMR
                     LFAEVVSEGGAAVIWADDGWSAAAAEVARARGLRVLG"
BASE COUNT         1480 a         3223 c         3213 g         1471 t
ORIGIN      
        1 ccggggtgcc ggccaaaagg cccagctcca ccatggcgat cgcctcgccc gaaaggccca
       61 gcagggcgcc ggcgggcagc gctgccagca gcagcggcgg ggcaaagctc agccagtggg
      121 cggcgatgcg cacggccacc acgccttcct cggccagccc gcgcagggcc cattggtcga
      181 agaagccctg gtcgagatcg ggctccacca gccggtcgat cggcaggatc gccgccagca
      241 gcgccgccac ccacagcacg ccgccgccgc agcgcgccag cagcgccgca tccggcccca
      301 cggcaaaggg aaacaccacc gccaccgcca ggaagaacag caccggcagc agcgcgccgc
      361 cctgccgccc gcccagcagc aacaccgcca gatcgcgccg gaaaatcgcc cacagcgccg
      421 ctctcatgcc cattccccgc tctccggcgc gggcacgaag ccggccagct ccagccgggg
      481 cagcgcgccg gccggcaggg caaagggctg gtgcgaggcg atgaggcagg cgccgcccgt
      541 gccgatatgc tctgccacca gcgcgcccac cagcgcgcag ccctgcgtgt ccagcccgtt
      601 cagcggctcg tccagcagcc agatggccgc ctgctggccc agcagccgcg ccagcgccgc
      661 ccgcttgcgc tggccggttg acagatagcg caccggcacg tcgagcagat cggcaaggcc
      721 cagccgcgcg gcatgggggc cgggatcagc gccgtcgatc ccggcccaga aggccagcgc
      781 ccggcccagc ggctggctgc catccagcgc cagcttttcc tgcaccagcg ccaccgggcc
      841 atggcgttcc accgtgccgg caaagggcgc cgccagcccg gccagaatgc gcatcaggct
      901 ggatttgcca atgccattgg cgccggccag atgcagcgcc gcgcctggcg ccagcgccag
      961 atccagcccg gcaaacagca tcctgtcggc ccggcggcag gccagcgcgc gggcgctcag
     1021 caggagaggg gaggcttgca tcgcgcccgg cgatagggga aagctgcccg cctgccaagc
     1081 cgaacgcaag aggtgcccgc catgcccgcc tgcccgccgc tgctcaccgc gcccgaatgc
     1141 gcagaggcgc tggccgccct gccactgtgg cggctggccg acgaggggcg ggcgatcgcg
     1201 cgcgatctcg tctttgccga ttttgccgcc gcctttgcct tcatggccca tgtggcgctg
     1261 ctggccgagc gggccgacca ccacccggac tggcgcaatg tgtggaaccg ggtggagatt
     1321 ggcctcacca cccatgccgc cggcgggctg acgcggcgcg atatcgccct ggcgcaggcg
     1381 attgaccggg tggcgggcgg ctaaagcgcc ggattgtcgt agcaatgccg gcgctctgtg
     1441 cggttgtgca gcgttgcgcg ggcgtttgag cagcttgctt gcggtgattt ttcgaggtga
     1501 acgataagac gaaaacaaaa ttggacttta tagcggacgc gggcagccag ccaaatcaac
     1561 gcgaatgatg cctgaggttt gcctgtggct catcgcccga atgccctgct tgtccaaccg
     1621 acagctttgg ttgccgcctc gccactctat cgacgctatc ttctcgacgg gttcggtcag
     1681 gtccaccccg ttgccgggcg caacctcacc acgcccggtg gggattgttg ggtcctcaaa
     1741 ggtgatgaca accgacaatg ggcctgtggt ttggttttcg acagatgtgc agaacgcctc
     1801 atgacatgcg gatagacctg ccgccgctat caacaggcat cgagcgggca tgcgaaggga
     1861 cattcgccca aggtaacaca aacaggaaaa tccacaaaga tgggtcgtgt ctctacagcg
     1921 ccggattgtc gtagcagtgc caggtgaaca gggccatggc gccgcgatgc gggcgccagc
     1981 tttcggccat ggcgcgggtg ggggcttccc gagggcgttc gggcagggcc agcagcttgt
     2041 gcaggcccgc ctgcacgccc agatcgcccg ccggccagat atcggcgcgc ccttcggcaa
     2101 acagcagata gatctcggcc gaccagcgcc cgatgccctt ggccgccgtc agcgcggcaa
     2161 tggcggcttc atcctctgcc ggcagggcgg cgaggtccag cgtgccatcg gccaccagcg
     2221 cgcacaggct gcgcgcatag cccgccttct ggcgcgagag gccgcaggcg cgcagcgcct
     2281 cgtcgtcggc cgccagcagc gcctgggccg gcacgcctgc ccccagcgcc gcttccagcc
     2341 gcgcccacac gctggccgcc gccgccacgc tcacctgctg gccaacgata gtgcgcagca
     2401 aggtggccag cccccgcgcg cggatccgcg gcgccggata gcccaccacc gccagcgcgc
     2461 gggccacggc cggctcgcgc atggccagcg catccagccc ggtgcggatc gtctcggctt
     2521 caaggcccat cggcttgctc ctgccggctc gcctgcattg gcgcaaccgg ccaaagcgtt
     2581 ggcgcaggtt gccaaagcat gcaactgcga ttagcagccc agatgaaagg ccatcagggg
     2641 ccgatgagga aaaggatcca agcaatgccg aagttggtgg tcgtcaaccg tgccggcgag
     2701 gaaaccacgg tggacgccga agtgggccgc accgtgatgg aagtggtgcg cgacaacggt
     2761 tttgacgaga tgctggcgct gtgcggcggc tgctgctcct gcgccacctg ccatgtgcat
     2821 gtggacccgg cctttgccga caagctgccg gcgatcaccg ccgacgagga cgacctgctc
     2881 gacagctcgg accaccgcga cggcaactcg cgcctggcct gccagctgcc gatcaccgag
     2941 gcgctggaag gcctgaagat caccatcgct caggaagact gagcctgcca ccggggcatg
     3001 ccccggccgc cagggacaaa atcccctggc aatagcctga ctttaggggc gggcagcgca
     3061 attcgtttgc gctggcccgc ccctgcttct aggcgaggac gcatgactgt ggcacccgtt
     3121 tcttccgaca cgcttggcct tatcggcaac accccgctcg tgcttctgca gggccccagc
     3181 gaggcggccg gctgcgagat ctggggcaag tgcgaatttg ccaatccggg ccagtcggtg
     3241 aaagaccggg cggcgctgtg gatcgtgcgc gatgcggaaa agcgcgggct gctgaagccg
     3301 ggcggcacga tcgtggaagg caccgcgggc aacaccggca tcggcctggc gctggtggcc
     3361 aatgcgctgg gctacagaac gatcatcgtc atgcccgaga cgcagagccg cgagaagatg
     3421 gacaccttgc gtgccctgcg cgccgagttg gtgctggtgc cggccgcgcc cttttccaat
     3481 ccgggccatt tcgtgcacac ctcgcgccgg ctggcggaag agaccgaggg cgccatctgg
     3541 gccaaccagt tcgacaatat cgccaaccgg cgcgcccata tcgaatcgac cgcgcccgag
     3601 atctgggcgc agatggaagg gcggatcacc ggcttcacct gcgctgcggg caccggcggc
     3661 acgctggccg gcgtggggct gggcctcaag gcgttcgacg agaatgtggt gatcgcgctg
     3721 accgacccgc atggcgccgc gctctacaac tactacgccc atggcgagct gaaggccgag
     3781 ggcagcagcg tggccgaggg catcggccag ggccgcatca ctgccaatct cgatggcgcg
     3841 ccggtggaca cgcagttccg catcgccgat gccgaggggc tgcactgggt ggagcggctg
     3901 ctggccgagg aagggctggc gctgggcctt tccagcggca tcaatgtggc gggcgcagtg
     3961 gcgctgggcc gccagctggt ggccgagggc cgcgaggatg tgcgcatcgc caccatcctg
     4021 tgcgacaccg gctttcgcta cctctccacg ctctacaacc gcgcctggct ggaagccaag
     4081 gggctgccgg tcttcccctg gctggcctga tcggctaggc tgcgggcatg agcaaggccc
     4141 cgcgcccttc cgccctttcc gcagagccga cctatgccgg cgcccccacc gcgcgggaat
     4201 tgcgcgcgca ggcggtgcag cggctgcagg tggggctgtt tggcctggcg ctgatgctgc
     4261 tgctggtggg cctggccaat atcatcatgg accatgcccg ccagaacgag acggcggccg
     4321 gcgctgccat tgcggtatcc agcgcttctt cggccagcgg cggcagcacc gatccgctgg
     4381 ccgatatcgg cgtggtgccc tcgccggatg ccgatccggc cgcgcaccgg cccaccagcc
     4441 gcaccgcgcc cacgcgctga tttcctgaaa ctgctgcctg aggccacgcg gcgatgccgc
     4501 cggggccccc ttgcgcctgc gcgacaacgc ccctgcgcct gcgcgcgact ctgccgggaa
     4561 agggctgtgg gtgttttgcg ggaaaatgcg ggaagcgcct gattctgccc cgccgaatgg
     4621 atcttgttta cggaaattta cagcggtttt ggtgctaatt gcgtattatc cggctgcggc
     4681 gttaacctgt ttgcaaccaa acggcatttc cccatctgaa aaatattgta ataaatcaga
     4741 tacataattt aaggcactcc gcaaagtggc ggaaattccc gcattgtggc ttccctgccc
     4801 gtcccggacg cgatacatct tggcttctgg gaaagtttcg catgccgctg ggcagccggt
     4861 ttcccgccgg ggccggcagc aggcgttccc tttcatgtcg gggtttctag cggggtaggc
     4921 aggggcgtga ctggtcagcc ggtcatctac agcgggcagg gcttctcgcc gaagggcgac
     4981 aaggaacgct tcgtgctgcc cgccggcttc cgccgcgccg tcagcgaatc gagcgctggc
     5041 gccaatgtgc tgctggtctc caagcacagc agctggccgt gtctcatcgg ctatggcctc
     5101 tcgcgccgcg acagcctgat ccagcagatc gaccgcgagg aagagatggc cgcccgcgcc
     5161 ggccagccct tcgaccgcga catgcgctat ttccagctgt tcgattgcgc cgagatcagc
     5221 ttcgatggct ctggccgctt catcatcccg gcccatctgc gcaagctcgt cggcattggc
     5281 gaggcgatct atttccaggg ctccggcccc ttcttcaccc tgtgggcgcc cgatgtgctg
     5341 gccgcccagg aaggcccgct gtgggccagc gcccaggccg cctgcgccag cctgctggaa
     5401 gagccgggga ggggccgcaa atgagccttt ccggccccta tgacctgctg cccgcagatg
     5461 cgccgcacat gccggtgctg ctggccgagg tggttgccgc tttggccccg gcgccgggcg
     5521 cggtgatcgt cgatgccacc tttggcgccg gcggctacac ccgcgcgctg ctggccgccg
     5581 gcgccaccgt ccacgcgttc gaccgcgacc ccgatgcgat tgccgcggcc cgcctgtggc
     5641 ccgaaacgca aggcgatgcc ccccggctgg tgctgcacca ccggccgttc tcggaactgg
     5701 ccgcggggct ggccgaggcg ggggtggccg cgatcgatgc ggtggtggcc gatatcggcg
     5761 tgtcgtccat gcagctcgat caggcggggc gcggctttgc cttctcggcc gatggcccgc
     5821 tcgacatgcg gatggcgcag gaagggccct cggcggccga ttttctcaac accgccagcg
     5881 aggaagcgat tgccgacgtg ctgttccatc tgggcgagga gcgccagtcg cgccgggtgg
     5941 cccgggcgat tgtggcggcg cggccgctgg ccaccaccgg cgatctggcc cgcgtggtgc
     6001 gcaaggcgct gggccacaag ccgggcgcgc ccaaggatcc ggccacgcgc agcttccagg
     6061 cgatccgcat ccatgtgaac gacgagctgg gcgagctgaa cgcgctgctg gatgccgcaa
     6121 cgggcctgct ggcgccgggc gggcggctgg cggtggtcag cttccacagc ctggaagacc
     6181 gcatcgtcaa gcagttcctg cgcgaggcga gcggcgccct gccggcagcc tcgcgccatc
     6241 tgccgatggc cgctgccggc cccgccccgc tttacgaacg cgtcagcaag gccatccgcg
     6301 ctggcgaggc agagctggcc gccaatccgc gcgcccgttc cgccaccctg cgccacgcta
     6361 tccgcaccac cgctcccgcc ccttccgatg cccacaggag agccgcatga acatgacccg
     6421 agaccgcttg cacgcgattg gctggggcat gacgctgacg gtatgcctgg cgctgctggt
     6481 ggccttgacg atgcgcgtca atgcggtgaa gagcgaggtg catctggccg atcgccagat
     6541 cgccaccctg cagcaggaaa agatgttcct cgagaccgaa ttcgagacgc gctccagcca
     6601 gcagcagctg cgcgccttca acgatgtcga ttttggctat gaggcgccca cggccggcca
     6661 gtatctggaa agcgagcggc agctggcagc gctgggcaag ccggccgggc ccgatgcgcc
     6721 cgcgcccgtg cgcatggcca gcgcggatga tggcgccggg cagggcgccg gcgatgccca
     6781 gccggtgcgc gcgctggtgg cgctggccgc cgaaaaggat gcccagccgc acaagctggc
     6841 tgatcgccac ccctcccgcc cggcccggca cagcgaggcc gtggaggaat gacaggcatc
     6901 gccggcccgc agatcagcgc ggtcatttcc tcggttcgtt cggatagtgt cgttcatcgt
     6961 cagcgtgcgc tggcgatggc ccgttttcgg ctgctgtggg tgttgatcgg ctttgcggcg
     7021 ctggctttca tcgcgctggc ccggttgacg cagctgggct ttgcggcggg cgatcgcggc
     7081 aatggcgcgg ccggcatggc agtggacagg ggcgatatcg tcgatcgcaa cggggtgccg
     7141 ctggcgcgcg cgtttccggc ctattcgctg tggttcaacc ccaatgcgct gcatggcggg
     7201 ccggcgctgg tgaaatcccc gcgcgaggtg gcagaggcgc tggtgcgcat cttcccggat
     7261 gagaatgtcg atgatctgac gcggcggctg gccgctggca agccgggcta tctcaagcgc
     7321 cgcatcctgc ccgaggatgc caaccgcatc cacgcgctgg gcgagccggc gctggaattc
     7381 ccgcgtgaga acgagcgcta ttatccgcag ggctcgatgg cggcgcatgt gctgggctat
     7441 gtcgattccg acgggcacgg ccaggttggt atggaaaagg tgttcgatca ccagctgacc
     7501 gatcccgtca cacgcggcaa tccgctggtg ctgtcgatcg atgcgcgcgt gcagggcgtg
     7561 ctggaagacg agctggccaa ggggctggcc cgcgcccagg ccaaaagcgc gggcggcatc
     7621 atcatggatg tggaaaccgg cgaagtgctg gcgctggcct cgctgccttc cttcaacccc
     7681 aatctgatcg accggcaggg ctcggaacac gccttcaacc tggtggtcag ccagccctac
     7741 gagctgggca gcgtgatcaa gccgatcacc gttgccactg ccatcgaggc gggcgtgatc
     7801 accagcatgg cgcggcgcta tccggcgggc aagccgctgt cgatcgccgg tttcgagatc
     7861 catgacgacg agaatttcgg cgattcgctg aatatccccg aagcgctgat ccattcctcg
     7921 aacatcgtgc tggcgcaggt gggcgatcag ctgggcgcca cgcgcctgcg cacggcgatg
     7981 gaaaagctgg gctttgccga gcgcccctat atcgagctgc ccgcccgcgg cctgccgcgc
     8041 ttcccccatg ccgatcacgg cgaatggtcc cgcgtgacga cgatgaccgc ctctttcggc
     8101 catggtttcg cgatcactcc gctgcatctg gcctgtgcct atgccgcgct ggtgaatggc
     8161 ggcatctggc atccggccac cttgcagaag ctggcgccgg gccaggcacc ggcggggcgg
     8221 cgggtgttct cggcggccac cagcgcgcgc atgcgccagc tgctgagggt gatcgtggaa
     8281 gtgggtacgg gccgttcggc caacgcaccg ggctttcgcg tgggcggaaa gacgggtacg
     8341 gccgagcgat ctgaccgttt cggatatcat cgcgatcagg tggtggcgac ctttgccagc
     8401 gccttcccga tggatcatcc gcgctatgta gtgattgcca tgctcgatac gccccaggcc
     8461 aatgccgcca ccagctacca gcgcaccgcc gcctggaatt ccgcgccgat cgtgggtcgg
     8521 gtggtgccgc gggtggggcc cttgctgggc atcatgcccg atcagacgcg cgacatcgac
     8581 atttccgacc tgaagccgct gatcgccaat ggagatggcg aatgaagctg gcagcgcttc
     8641 tggccgaggc cggccttgcc cctgtggcgg gcgccgaggc cgccgctgtc agcggctttg
     8701 cgatcgacaa tcgcaaggtg gcgccgggca atgtgtttgg cgcctttgcc ggcgctgtgg
     8761 tgaacggcga ggatttcatt ccggccgccg tggccgcggg cgcggtggcg gtggtggccc
     8821 ggccgcaggc ccgggtgacg ggcgcgctgc atatcgcggc ggacaacccg cgccgcgcct
     8881 ttgcccgcct ggccgccggt ttctatcgcc cggtgcccgc cactatcgtt gccgtgaccg
     8941 gcaccaacgg caagaccagc acggtggaaa tgctgcgcca gatctggcgc atggccggcc
     9001 agcgcgcggc cagcatcggc acgctgggca tcaccacgcc cgaggagacc acctataccg
     9061 ggctgaccac gcccgacatc gtcaccttca tggccaatat ggccggcctt gcgcgtgaag
     9121 gggtcagcca tgttgcctat gaggcatcga gccatggcct cgctcaatat cgcaacgagg
     9181 ggctgccggt ggcggccggc ggcttcacca acctcagccg cgaccatctc gattatcacg
     9241 ccacgatgga ggcctatttc gccgccaaga tgcgcctgtt cgcagaggtc gtctcggagg
     9301 gcggggcggc ggtgatctgg gcggatgatg gctggagcgc ggccgccgcc gaagtggcgc
     9361 gcgcccgggg cctgcgcgtg ctgggcg
//
LOCUS       DASRYE010000175         5853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_133843,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000175 DASRYE010000000
VERSION     DASRYE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133843"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VFF98_09135"
     CDS             complement(<1..1382)
                     /locus_tag="VFF98_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZU63835.1"
                     /translation="MIAKALKNIRSRPARRCAALAALAATLAGVAPAAHANSAAADYF
                     MGRAARSAVPSLLSEDERAYYRDLFGAIKQQDWARVQALFDQRADGPLHAVARAEYFL
                     APGSPKIDGPTLGLWLATGGDLPEADQITTLALKRGVALTLPLPPAQPFMALPTAPRR
                     VRPKSIADGTMPANVSAAILDRIKADDPAAAQLLLDGVGATLSGEARTEWAQKVAWAY
                     YINNRDNDAYQLATSAGQGSGAWVSEAWWVAGLAAWRQDDCAAAEDAFANAARSAGNG
                     ELAAASSYWQSRAAVRCGRPEAAAAPLRAAARADETLYGMLAAEQLGLKLPGTHAAPD
                     FTTADWQALREVGNVRVAVALSQIGEDALADEVLRHQAAIGAVEQYQPLSRLARDLGL
                     PATQLWMAYNAPVGGAPAPASRYPTPKWQPATGWQVDPALVLAHTLQESQFRAAVISP
                     AGARGLMQIMP"
     gene            complement(1457..2239)
                     /locus_tag="VFF98_09140"
     CDS             complement(1457..2239)
                     /locus_tag="VFF98_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZU63836.1"
                     /translation="MGIDYTFDEYCADLELDAALAAAQGWWQLAGLSHDYADAPRAWL
                     APPEEAAQPGEPAARRRPAPPPPEPEQPPALLAGEGAGWPQDLAAFHSWWLAEPALDE
                     GRLAGRVPPRGAHGAQLMVIIAQPEAEDGETLLSGPEGRLLAAMLAAMGMAPDATYVA
                     SVLPRFTPAPDWRAAATAGLGAVLARHVDLVGPARVLLLGEDILPLLAHDPTQNTAVF
                     TGLNQKPITVPLLAGRSLGFMLGRPMAKAHLWRRWLEWDGPE"
     gene            2369..4018
                     /locus_tag="VFF98_09145"
     CDS             2369..4018
                     /locus_tag="VFF98_09145"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZU63837.1"
                     /translation="MTIERESMPYDVVIVGGGPAGLASAIRLKQLNGDLSVCILEKGS
                     EVGAHILSGAVVDPRALDELLPSWREEGCPMAETPVTDNWHWVLTKSGKYAMPHALMP
                     PFMSNDGNYTGSLGNLCRWLAGKAEELGVEIFPGFPAAEVLYGEKGEVIGVQTGDMGI
                     ARDGSEKGDFQPGMNLLAKYTLFCEGARGHLTKRLRAKYDLERDCQPQVYGLGIKELW
                     DIDPAKHVPGRVLHTQGWPLSESGSWGGGFLYHQANNQVALGFVTALDYKNPYVYPFE
                     EFQRWKQHPEIRAILEGGKRVAYGARAINEGGWQSVPQLAFPGGVLAGCSAGFVNVPR
                     IKGSHTAMKSGMLAAESIVAAIAAGREGDALAEYDAAVRASWIAEELKLVQNAQPMVA
                     KFGGTLGTILAGADMWSRHLFGFGVWKALAHHTDAAATGKAKDYQPIAYPKPDGQISF
                     DRLTSVSFSFTNHEEDQPCHLRLKSEAVPVQVNLAQFAGPEARYCPAGVYEFVGEEGD
                     KRLQINAQNCLHCKTCDIKDPTQNIDWVAPEGGGGPNYPNM"
     gene            4130..4969
                     /locus_tag="VFF98_09150"
     CDS             4130..4969
                     /locus_tag="VFF98_09150"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZU63838.1"
                     /translation="MTAAAQTAVLTETAYAKINLALHVRARRADGYHELETFFAFVDA
                     GDVLEARPADADVLELHGEFAALLAGETSNLVTDALALLPHGGAGWHVRLDKRLPVAA
                     GLGGGSADVGAVFRMVARAHGLPDGWPALAARLGADVPACVDSLPVIGRGTGTELAPA
                     ATDLAGCPVLLVNPRLPVPTGPVFKAWDRVDRGPMPEGSARAVMLEGRNDLEAPAITI
                     CPAVADVLAALRHGGEAWAARMSGSGATCFALYDSDAARDAAAARIAASRPGWWQMAG
                     RLR"
     gene            4969..5691
                     /locus_tag="VFF98_09155"
     CDS             4969..5691
                     /locus_tag="VFF98_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZU63839.1"
                     /translation="MAAFELLGTPTPGGLLIVVDHASDHVPADIDLGIAPALLATHIA
                     VDIGVAAIARAMLGAGTAAFLATASRLVCDLNREAHAPAIIPIASDGHAIPGNALDHA
                     GHEARLARFYHPYHTALEASIAAVRPGLILSLHSFTPSLASHPQQQRPWHVGVLYNAD
                     ERAPRLALPLLAAEEGLVVGDQQPYSGRLLNATMNRHAEGHGIPYLGVEVRQDLIADA
                     AGQGQWAARLGRLCGQVMGALA"
BASE COUNT          883 a         1959 c         2115 g          896 t
ORIGIN      
        1 ggcatgatct gcatcagccc gcgcgcgccg gccgggctga tgacagcggc gcgaaactgc
       61 gattcctgca aggtgtgggc cagcaccagc gccggatcca cctgccagcc ggtggccggc
      121 tgccacttgg gcgtgggata gcggctggcc ggtgccggcg cgccgcccac aggggcgtta
      181 taggccatcc acaattgcgt ggccggcagg cccagatcgc gcgccaggcg cgacagcggc
      241 tgatattgct ccacagcgcc aatggcggcc tgatggcgca gcacttcgtc ggccagcgca
      301 tcctcgccga tctgcgagag ggcgaccgcc acgcgcacat tgcccacctc gcgcagcgcc
      361 tgccagtcgg ccgtggtgaa atcgggggcg gcatgagtgc cgggcagctt cagccccagc
      421 tgctcggccg ccagcatgcc ataaagcgtt tcatcggcgc gggcggcggc gcgcaagggg
      481 gcggccgctg cttccggccg gccgcaacgc accgccgcgc ggctctgcca ataagaggag
      541 gcggccgcca gttcgccatt gccggcgctg cgggcggcat tggcaaaggc atcctcggcg
      601 gcggcgcaat cgtcctgtcg ccaggcggca aggccggcca cccaccatgc ctcgctcacc
      661 caggcgccgc tgccctggcc ggcgctggtg gccagctgat aggcgtcgtt gtcgcggttg
      721 ttgatgtaat aggcccaggc cactttctgg gcccattcgg tgcgggcttc cccgctcagc
      781 gtggcgccca cgccatcgag cagcaattgc gcggcggccg gatcatcggc cttgatgcga
      841 tcgaggatgg cggccgagac attggccggc atcgtgccat cggcaatgct tttggggcgc
      901 acgcggcggg gcgctgtggg cagggccatg aacggctggg ccggcggcag cggcagggta
      961 agcgccacgc cccgcttcag cgccagcgtg gtgatctggt cggcctcggg cagatcgccg
     1021 ccggtggcca gccacaggcc cagcgtgggg ccatcgatct tcgggctgcc gggggcgagg
     1081 aaatattccg ccctggccac ggcatgcagc gggccatcgg cgcgctggtc gaacagggcc
     1141 tgcacgcggg cccagtcctg ctgcttgatg gcgccgaaca ggtcgcggta ataggcgcgc
     1201 tcgtcttcgg acagcaggct gggcacggcg ctgcgggcgg cccggcccat gaaataatcg
     1261 gcagcggcgg aattggcgtg ggcggcgggc gccacgccgg caagggtggc ggccaaagcg
     1321 gcaagcgcag cgcagcgccg ggcagggcgg ctgcggatgt ttttcagggc cttggcaatc
     1381 acgcgcgaac acactccttg ctcggcagcg atcggctccg gcctggtgtt cagcccgccc
     1441 cccgtttctg cccgttctat tccggcccgt cccattccag ccagcgccgc cacagatggg
     1501 ccttggccat cggccggccc agcatgaagc caaggctgcg cccggccagc aggggaactg
     1561 taatcggctt ttgatttaat ccggtaaaaa cggcagtgtt ttgcgtcgga tcgtgcgcga
     1621 gaagcggcag gatgtcctcg cccagcagca gcacgcgggc cgggcccacc agatcgacat
     1681 gccgcgccag caccgcgccc agcccggcag tggcggccgc gcgccaatcg ggcgcggggg
     1741 tgaagcgcgg cagcacgctg gccacatagg tggcatcggg cgccatgccc atggccgcca
     1801 gcatggccgc cagcagccgc ccttccgggc cggagagcag ggtttcgcca tcctccgcct
     1861 cgggctgggc gataatcacc atcagctgtg cgccatgggc cccgcggggc ggcacgcggc
     1921 cggccaggcg gccttcgtcc agcgccggtt cggccagcca ccagctgtgg aaggcggcca
     1981 gatcctgcgg ccagccggcc ccctcgcccg ccagcagggc agggggctgc tcgggctctg
     2041 gcgggggcgg ggcggggcgg cggcgggcgg cgggttcgcc gggctgggcg gcttcctcgg
     2101 gcggggccag ccaggcgcgc ggggcatcgg cataatcatg gctcagcccg gccagctgcc
     2161 accagccctg cgcggcggca agggcggcat ccagttccag atcggcgcaa tactcatcaa
     2221 aggtgtagtc gatgcccata agctttcctt acagacttga ccttgccgcg gcgagctttc
     2281 aattgcccga ttaagatcgg cccatacatc ggcccagcaa aagggggccc tgcggcagcg
     2341 cggggcgcgg acagacggga taaagagcat gacaatcgaa cgggaatcga tgccgtatga
     2401 tgtggtgatt gtgggtggcg ggccggcggg cctggcatcg gcgatccggc tgaagcagct
     2461 gaacggcgat ctgtcggtgt gcattctgga gaagggctcg gaagtgggcg cccatatcct
     2521 gtcgggcgcg gtggtggacc cgagggcgct ggacgagctg ctgccgagct ggcgcgagga
     2581 gggctgcccg atggcagaga cccccgtcac cgacaactgg cactgggtgc tgaccaagag
     2641 cggcaaatat gccatgccgc atgcgctgat gcccccgttc atgagcaatg acggcaatta
     2701 caccggcagc cttggcaatc tgtgccgctg gctggcgggc aaggccgagg aactgggggt
     2761 ggagatcttc cccggcttcc cggcggccga agtgctgtat ggcgagaagg gcgaggtcat
     2821 cggcgtgcag accggcgaca tgggcatcgc cagggatggc agcgagaagg gcgatttcca
     2881 gcccggcatg aacctcctgg cgaaatacac gctgttctgc gagggcgcgc gcggccatct
     2941 caccaagcgg ctgcgggcga aatatgatct ggagcgcgat tgccagccgc aggtctatgg
     3001 cctgggcatc aaggagctgt gggatatcga ccctgccaag cacgtgcccg gccgggtgct
     3061 gcacacgcag ggctggccgc tgtcggaaag cggcagctgg ggcggcggct tcctctacca
     3121 ccaggccaac aaccaggtgg cgctgggctt cgtcaccgcg ctcgattaca agaaccccta
     3181 cgtctatccc ttcgaggaat tccagcgctg gaagcagcac cccgagatcc gcgcgatcct
     3241 cgagggcggc aagcgcgtgg cctatggcgc gcgcgccatc aacgagggcg gctggcagag
     3301 cgtgccgcag ctggccttcc cgggcggcgt gctggcgggc tgttcggccg gctttgtcaa
     3361 cgtgccgcgc atcaagggca gccacaccgc gatgaaaagc ggcatgctgg cggccgaaag
     3421 cattgtggcg gccattgccg ccggccgcga gggcgatgcg ctggccgagt atgacgcggc
     3481 cgtgcgcgcc agctggatcg ccgaggagct gaagctggtg cagaacgccc agccgatggt
     3541 ggccaagttt ggcgggacgc tgggcaccat cctggccggc gccgacatgt ggagccgcca
     3601 cctgttcggc tttggcgtgt ggaaggcgct ggcccatcac accgatgccg cggccaccgg
     3661 caaggcgaag gattaccagc cgatcgccta tcccaagccc gatggccaga tcagcttcga
     3721 ccggctgacc agcgtctcct tctccttcac caaccacgag gaggaccagc cctgccacct
     3781 gcgcctgaag agcgaggccg tgccggtgca ggtgaacctg gcgcaatttg ccggccccga
     3841 ggcccgctat tgcccggccg gcgtttacga gttcgttggc gaggagggcg acaagcgcct
     3901 ccagatcaac gcccagaact gcctccactg caagacttgc gacatcaagg accccaccca
     3961 gaacatcgac tgggtggccc ccgaaggcgg cggcgggccc aactatccca atatgtgatc
     4021 cgcgcaaaag cggtgttggc caaaaggggt gggggagcga tggctctcct gccctttttg
     4081 ccatttccgc tcttgccccc gcgcctgccc cgcgccaaag cgcaaagcca tgaccgctgc
     4141 tgcccaaacc gccgtgctga ccgaaaccgc ctatgccaag atcaatctgg cgctccatgt
     4201 gcgggcccgg cgtgcggatg gctatcacga gctggagacc ttctttgcct tcgtcgatgc
     4261 cggcgatgtg ctggaagcgc gcccggctga cgcggatgtg ctggagctgc atggcgaatt
     4321 tgccgccctg ctggcgggcg aaaccagcaa tctcgtcacc gatgcgctgg cgctgctgcc
     4381 ccatggcggg gcgggctggc atgtgcggct cgacaagcgg ctgccggtgg cggcggggct
     4441 gggcggcggt tcggccgatg tgggggcggt gtttcgcatg gtggcgcgcg cccatggcct
     4501 gccggatggc tggccggcgc tggccgcccg gctgggcgcc gatgtgccgg cctgtgtcga
     4561 cagcctgccg gtgatcggcc ggggcaccgg caccgaactg gcgccggccg ccactgacct
     4621 tgccggctgc ccggtgctgc tggtgaaccc gcgcctgccg gtgcccacag ggccggtgtt
     4681 caaggcgtgg gaccgggtgg accgcggccc catgcccgag ggcagcgccc gcgcggtgat
     4741 gctggaaggg cgcaacgacc ttgaagcgcc ggcgatcacc atctgcccgg cggtggccga
     4801 tgtgctggcg gcgctgcgcc acggcggcga ggcctgggcg gcgcggatga gcggctctgg
     4861 cgccacctgc tttgcccttt acgacagcga tgccgcgcgc gatgccgccg cggcccgcat
     4921 cgccgcaagc cggcccggct ggtggcagat ggcggggcgc ctgcgctgat ggcggctttc
     4981 gagctgctgg gcacgcccac cccgggcggg ctgctgatcg tggtggacca cgccagcgac
     5041 catgtgccgg ccgacataga ccttggcatc gcgccggcgc tgctggccac ccatatcgcg
     5101 gtggatattg gcgtggcggc gatcgcgcgg gcgatgctgg gggcgggcac ggcggcgttt
     5161 cttgccactg cctcgcggct ggtgtgcgat ctcaaccgcg aggcccatgc gccggcgatc
     5221 atccccattg ccagcgatgg ccatgcgatc cccggcaatg cgctggatca tgccggccat
     5281 gaagcgcggc tggcgcggtt ctatcatccc tatcacacgg cgctggaggc ctcgattgcc
     5341 gctgtgcggc cggggctgat cctcagcctc cattccttca cgcccagcct tgccagccat
     5401 ccgcagcagc agcgcccctg gcatgtcggc gtgctgtata acgctgatga gcgcgccccc
     5461 cgcctcgccc tgccgctgct ggcggccgag gagggcctgg tggtgggcga tcagcagccc
     5521 tattcgggcc gcctgctcaa cgccacgatg aaccgccatg ccgagggcca tggcatcccc
     5581 tatctgggcg tggaagtgcg ccaggatctt atcgccgatg cggccgggca ggggcaatgg
     5641 gcggcccggc tggggcggtt gtgcgggcag gtgatgggcg ctcttgccta gggcgggggc
     5701 gcgggtgtag ggcgcggggg ttttctgtcc ctgatcctcg gagctgtctt atgcccgcct
     5761 atcgttcccg cacctccacc catggccgca acatggcggg cgcgcgtggc ctgtggcggg
     5821 ccaccggcat gaaggatgcc gatttcggca agc
//
LOCUS       DASRYE010000176         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_133855,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000176 DASRYE010000000
VERSION     DASRYE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133855"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="VFF98_09160"
     CDS             complement(<1..1414)
                     /locus_tag="VFF98_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZU63840.1"
                     /translation="MSRDATLTAPADTPATLATGRLERLFNPRSLVLVGASDSSAWSQ
                     LIHGNIVASGFAGPVYAVNRRGVPAHGRPGFTRCADLPAPPDLAYLFVPAEGVAKALA
                     DVAEAGIAHAIVLSSGFAETGAEGAAAQARLAALARQKGIALLGPNSLGFNNYGARVA
                     VSAFPVDGKAMAGGLSVISQSGATAHVIAQFARQQGIGLNCVVATGNEAGLGLVDVLD
                     HFVGDPATRAIALFAETIKDPAGFRQVAARASAAGKPIVVVKVGKSELASRLAEAHTG
                     AVTGDDAVFDAICRAHGVIRMDSLEAMVVAAGLMADVGEITGEIGLLSISGGACELIA
                     DRAAGAGLRLAPFTPATRARLEGVLSAYGAVFNPLDVTGAAVRDPSLFTQMLEILSAD
                     PAMGFLGVVQDVPPAEGDTLNEPILAAIGAGLAAARVPGTLINQALQPIGPYSRAVLA
                     RCAIPRAIGGVDHAVAALAAL"
     gene            complement(1411..3597)
                     /locus_tag="VFF98_09165"
     CDS             complement(1411..3597)
                     /locus_tag="VFF98_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU63841.1"
                     /translation="MPQLTAAPPLAPPRARAPQRAPADADMLAMLRASLRGRLGDLPV
                     GAGTAAVWPVLRELGVIPQGVALAVGGLAAGLLVAEELGRRALAAPWADGLAAAHAGL
                     ALEDRPLALAPAGTITLAGARASGTSAPFPWPGEGAAALLATAEGWAVVAPDAPGLRL
                     HRLTALNAEGQLARLELDAAPAERLPVSPAAAADALLVARLALLARALGAARAGFDLA
                     LEHARTRTQFGQPIGRFQAVQHKLANSFIALEGATAHLAAVAEAHDGDAAGWQARAEA
                     ALLFAGPALRQVALDNHHVLGAIGYAEEHAAPHLTRRIEIDTTLLGGTAAAREALAAH
                     VIDRAAPLVAPGAGPADGFRQELRHWLGQHWGPAQRAANLARPFSEQNWDAAFARALG
                     EAGLTTLTWPQAAGGRGASPLEKLAFLEETQAVAAPVQHTTVASWIVGPELLVHGNPR
                     LCAALLEPIRRGEVSFCLGYSEPGAGSDLAALATRARREGDEYVISGHKLWNTDGHRA
                     SHIILAARTDPDARPRHAGISLFLIPMDTPGISVRPGMAFYGHHFSEILLDEVRVVDW
                     MRLGPENGGWQILGKALANERIIMASFGTDLAVLLDAMMAAARAQGLLADGATRARLA
                     TLAAEVETARLLALGAILPRPDGPAPMVAAAVGKVFAGELGEALAQAGIELLGPAALL
                     SQGEEGALAGGSIEQMLRRAIMMVIGGGTAEIQRNIIALRGLELPK"
     gene            complement(3702..4514)
                     /locus_tag="VFF98_09170"
     CDS             complement(3702..4514)
                     /locus_tag="VFF98_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU63842.1"
                     /translation="MTHPTADSTLASAEETILLERPAPHVALVRLNRPARRNAINGAM
                     ARAIEAHVQAIEADTDIRVALIAAAPGPTFCAGADLAEAAAGRGHELMTAAGGFAGFV
                     EAPRRKPWIAAISGSALGGGLEIALSCDMIVCAESSVLGLPEVKRGLIAGAGGLFRLP
                     RALPRALALEMIATGEPIAAARALALGLVNAVVPAEAVEQAALDLAARIVVNAPLSVR
                     ESLAIARHAARSSDAAMLAESQAAADRLMPTHDWAEGPRAFVEKRAPQWRGC"
BASE COUNT          658 a         1721 c         1751 g          617 t
ORIGIN      
        1 gcagcgctgc cagcgctgcc accgcatgat ccaccccgcc gatcgcgcgg gggatggcgc
       61 agcgggccag caccgcgcgc gaataggggc cgatgggttg cagcgcctgg ttgatcagcg
      121 tgcccggcac gcgggcggcg gccagcccgg cgccgatggc ggccaggatc ggctcgttca
      181 gcgtgtcgcc ctcggccggc ggcacatcct gcaccacgcc cagaaagccc atcgccggat
      241 cggccgagag gatctccagc atctgcgtga acagcgaggg atcgcgcacc gcagcgcctg
      301 tcacatccag cgggttgaac accgcgccat aggccgacag caccccttcc agccgcgcgc
      361 gcgtggccgg cgtgaaaggc gccaggcgca ggcccgcccc cgcggcccgg tcggcgatca
      421 gctcgcaggc gccgcccgag atggagagca ggccgatctc gcccgtgatc tcgccgacat
      481 cggccatcag cccggcggcc accaccatcg cctccaggct gtccatgcgg atcaccccat
      541 gggcgcggca gatggcatcg aacaccgcat catcgccggt gacagcgccg gtgtgggcct
      601 cggccaggcg gctggccagc tcgctcttgc ccaccttgac gacgacgatc ggcttgccgg
      661 ccgcgctggc ccgggccgcc acctggcgaa agccggcggg atccttgatc gtctcggcaa
      721 acagggcgat ggcgcgcgtg gccggatcgc cgacgaaatg atcgagcaca tcgaccagcc
      781 ccagccccgc ctcattgccg gtggccacca cgcaattgag gccgatcccc tgctggcggg
      841 cgaattgggc gatgacatgg gcggtggccc cgctttgcga gatgaccgag aggccgccgg
      901 ccatggcctt gccatccacc ggaaaggccg agacggccac gcgcgcgcca taattgttga
      961 agcccaggct gttgggcccc agcagggcga tgcccttttg gcgcgcaagg gcagcgaggc
     1021 gggcctgcgc ggcggcgccc tcggcccctg tctcggcaaa gccggaggac agcacgatgg
     1081 catgggcaat gccggcctcg gccacatcgg ccagcgcctt ggccacgcct tctgccggca
     1141 cgaagagata ggcaaggtct ggcggcgcgg gcaggtctgc gcagcgggtg aagccggggc
     1201 ggccatgggc cggcaccccc cgccggttga cggcatagac cggccctgca aagccgctgg
     1261 caacgatatt gccatggatc agctgcgacc aggcggaact gtcggaggcg cccaccagca
     1321 ccagcgagcg ggggttgaac aggcgttcca gccggccagt ggcaagggtg gcgggggtgt
     1381 cggcgggggc ggtcagtgtg gcatcgcggc tcatttcggc agctccagcc cgcgcagggc
     1441 aatgatgttg cgctggatct ccgccgtgcc gccgccgatc accatcatga tcgcgcggcg
     1501 cagcatctgt tcgatggaac cgcccgccag tgccccttcc tcgccctgcg agaggagcgc
     1561 ggccgggccc agcagctcga ttccggcctg ggccagcgcc tcgcccagct caccggcaaa
     1621 gaccttgccg acagcggccg ccaccattgg cgccgggcca tcgggccgtg gcagaatggc
     1681 ccccagcgcc agcaggcggg cggtttccac ctcggcggca agggtggcca ggcgcgcgcg
     1741 ggtggcgcca tcggccagca atccttgcgc ccttgccgcg gccatcatcg catccagcag
     1801 cacggccaga tcggtgccga agctggccat gatgatccgc tcgttggcca gcgccttgcc
     1861 caggatctgc cagccaccgt tttccggccc cagccgcatc cagtcgacca cgcgcacttc
     1921 gtcgagcagg atttcggaga aatggtggcc atagaaggcc atgcccggcc gcacgctgat
     1981 gccgggcgtg tccatcggga tgaggaacag gctgatgccg gcatggcggg ggcgggcatc
     2041 gggatcggtg cgggcggcca ggatgatgtg cgaggcgcgg tggccatcgg tgttccacag
     2101 cttgtggccg gagatgacat attcatcgcc ctcgcgccgg gcgcgggtgg caagggcggc
     2161 aaggtccgat ccggcccccg gctcgctgta gcccaggcag aaggacacct cgccgcggcg
     2221 gatgggttcg agcaaggcgg cgcacaggcg cgggttgcca tgcaccagca gctccggccc
     2281 gacgatccag ctggccacgg tggtgtgctg cacaggcgcc gccacggcct gggtttcctc
     2341 cagaaaggcc agcttttcca gcgggctggc gccgcgcccg ccggccgcct gcggccaggt
     2401 cagggtggtg aggccagcct cgcccagcgc gcgggcaaag gcggcatccc agttctgctc
     2461 gctgaagggg cgggccaggt tggccgcgcg ctgcgccggg ccccagtgct ggcccagcca
     2521 gtggcgcagt tcctgccgga agccatcggc agggccggcg cccggggcca ccagcggcgc
     2581 tgcgcggtcg atcacatggg cggccagcgc ctcgcgcgcg gcagcggtgc cgcccagcag
     2641 ggtggtgtcg atctcgatgc ggcgggtgag gtggggggcg gcgtgttcct cggcatagcc
     2701 gatggcgccc agcacgtggt ggttgtccag cgccacctgc cgcagcgccg ggccggcgaa
     2761 cagcagcgcc gcctcggccc gcgcctgcca gccggcggca tcgccatcat gcgcctcggc
     2821 cacggcggca agatgggcgg tggccccttc cagcgcgatg aaactgttgg ccagcttgtg
     2881 ctgcaccgcc tggaaacggc cgatcggctg gccgaactgg gtgcgggtgc gggcatgttc
     2941 gagcgccaga tcgaagccgg cgcgggccgc ccccagcgcg cgggcgagga gggccaggcg
     3001 cgccaccagc agcgcatcgg cggccgcggc cgggctcacc ggcaggcgtt cggcgggcgc
     3061 tgcgtccagc tccaggcggg cgagctggcc ttcggcgttc agcgcggtca gccggtgcag
     3121 gcgcaggccc ggcgcatcgg gcgccaccac tgcccagccc tcggccgtgg ccagcagcgc
     3181 ggcggcgcct tcgcccggcc aggggaaggg ggcgctggtg ccactggccc gcgcgcccgc
     3241 cagcgtgatg gtgccggcag gggccagcgc cagcggccgg tcttccagcg ccaggcccgc
     3301 atgggcggct gccagcccgt cggcccaggg ggcggccagc gcgcggcggc ccagctcttc
     3361 tgccaccagc aggccggcgg ccagcccgcc caccgccagc gccacccctt gcggaatgac
     3421 gcccagctcg cgcagcaccg gccacaccgc cgccgtgccc gcgcccaccg gcagatcgcc
     3481 cagccgcccg cgcagcgagg cgcgcagcat ggccagcatg tcggcatcgg caggcgcgcg
     3541 ctggggggcc ctggcgcgcg ggggggcaag cgggggcgct gcggtaagct ggggcatggg
     3601 tggatcctct cctgttatgg cctcagcctg cggaaagggg cgggcggcgt ccaatatgtc
     3661 ttgcgccggt tttgatatgg ttttcgtctt aaaaccggcg ctcagcagcc ccgccattgc
     3721 ggtgcgcgct tttccacgaa ggcgcgcggg ccctcggccc agtcatgggt gggcatcagc
     3781 cggtcggcgg cggcctggct ttcggccagc atggcggcat cgctgctgcg ggcggcgtgg
     3841 cgggcgatcg ccaggctctc gcgcaccgac agcggcgcgt tgacgacaat gcgcgcagcc
     3901 agatcgagcg cggcctgttc caccgcctcg gccggcacca ccgcattcac cagccccagc
     3961 gccagcgcgc gcgcagcggc gatcggctcg ccggtggcga tcatctccag cgccagcgcg
     4021 cgcggcaggg cgcgtggcag gcgaaacagg ccgcccgccc cggcgatcag cccgcgcttc
     4081 acttccggca ggcccagcac gctgctctcg gcgcagacga tcatgtcgca cgagagggcg
     4141 atttccagcc cgccgcccag cgcgctgccg ctgatcgcgg cgatccacgg cttgcggcgc
     4201 ggcgcctcga caaagccggc aaagccgccg gccgccgtca tcagctcgtg gccgcgcccg
     4261 gccgccgcct cggccagatc ggcgccggcg cagaaggtgg ggccaggcgc ggcggcgatc
     4321 agcgccacgc ggatgtctgt gtccgcctcg atagcctgca catgggcttc tatcgcgcgg
     4381 gccatggcgc cgttgatggc attgcgccgc gccggccggt tcagccgcac cagcgccaca
     4441 tggggggcag ggcgctccag caggatggtc tcttcggcgg acgcaagggt gctgtcggcg
     4501 gtggggtggg tcatcgtggg gcctctcttc cggcgccggt ctggcgcccg gcaggggcgc
     4561 gccggctgtt gcggcgccag catgcgcccg gcgccggcca ttgcgccagc tgcgcaaatg
     4621 cctaggcttg gcgccatgga gatccacgcc cgcgccctgc gctatttcga catggtgcgc
     4681 cgcacgcgct cgatccgggc ggcggcgcgg ctgctgcatg tggcctcctc ggcggtgaac
     4741 cggcagg
//
LOCUS       DASRYE010000177        19348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_81346,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000177 DASRYE010000000
VERSION     DASRYE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19348
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81346"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2307)
                     /locus_tag="VFF98_09175"
     CDS             complement(<1..2307)
                     /locus_tag="VFF98_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63843.1"
                     /translation="MRCKRHCRRQGAAAIGLVLALGAASARADTGADTRADTGTQASP
                     AGEIVVTAQKRSQGINDVPIAISAFAGTTLADKGVQSLEGLSQVTPGLSVTATSASGV
                     PIYTIRGIGFSDYSTSASSTVGIYADEVAMPYAVMTRGAFFDTGQVEVLKGPQGDLYG
                     RNSTAGQINVNSARPTSTLQAGFAGDVSNYGETNLEGFVSGPLAEGLTARLAGKVSEG
                     GAWQRSISRPGDTLGNKDLMAVRGTIDWKATPTLDLFVSAHYIIDKSDNQAATAYNGQ
                     LIGQSALRLPVITSTAAGSPTAAFSTGNATAADWSDGIYRPRRNNRLAGFVARASLDL
                     PGMSLTSVTGYDRFTRRETNDGDGWAGNDSSQINDTQLTVISEELRLASRGNGPLSWI
                     VGGYVSHDTMHENYNYYMQQSYYALALGIQTLDTRYSQTTSSLAGFAHAEYRLGGGWR
                     LLGGFRYTHERRSWTGCTYDSGDGTLASFVGTTPGGCGTYDDIAGTPGYGTYTPFSDT
                     ITANRAMWKVGLDKKIGRSLIYGTISNGFKSGGFSGINTNLQSQLLPYRPETVTAYEL
                     GAKLSLLHNALRLNASAFWYDYRNKQEADYINTFVGALVQLTNVPRSRVRGVELEGHW
                     QASPRLSFDGSTTYLDAVVTDWPDAGVNNGANLAVATNLAGTRLANSPRWQSNLAGTY
                     EAPLNGRLTGFLTLDMNARTSSSGRVLALDPATAVPGYTLVNGRIGIKGEGDRWQLAL
                     WVRNLADRYYYTSAFVGNSVFVRMNGMPR"
     gene            complement(2437..3375)
                     /locus_tag="VFF98_09180"
     CDS             complement(2437..3375)
                     /locus_tag="VFF98_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU63844.1"
                     /translation="MTLRRETVSQSFERALRGVYGHSRFSADEIGEQSAFRVREDGQH
                     VLRPVMVYTRALTTQRAAHGLGDRWEGQFFLMQQISGQLGVHFDGGAPVVLRAGDLLL
                     MNPYQGCEFASRRANEVAVIPMPFDAAGGEAKVLAACGRRVDRQAAVGAVLGSALAAL
                     LAQRRAACAEERAVVGEVIHQLLGRLVAATPAGEQDLAADGLLRRAHRFALKELANPA
                     LSPDAIALGLGVSRRQLYRAFAEAGQTPASWLWSLRTEQAHLRLTSPQECAKSLTQLA
                     FDVGFNDMAHFSRSYRARFGCTPRQARLNAAGGAGG"
     gene            complement(3563..5236)
                     /locus_tag="VFF98_09185"
     CDS             complement(3563..5236)
                     /locus_tag="VFF98_09185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU63845.1"
                     /translation="MCALALPVCAQALPVVPAAAQTTAPPADRLFLHGQVLAPAGTAQ
                     ALALRGGVIVAVGSDAEILALPHAGAEVVDLAGRTLMPGLYDMHVHVMDAGLAERACH
                     LPQGGGAAAVKAAVQACARQVGPGGWILGGSWVAAALRPGEQTARLLDEAAPDHPVAL
                     SDEAHHSLWVNSRALAIAGITAATPAPAGGVIDRDAAGAPLGVLRETATALVESHIPA
                     ASPEEKLAAVEAATTEMLSYGIVGFTDAAVRPANAQALSAYARSGGLKQHARGCIVWG
                     PMSNGAEALIARRQELSAGPMRFDCVKIFLDGVPTESHTGAMVEPYAHTSGAHGILQV
                     PQARLNAAVAAFDAQGLEVKMHAAGDGATRAAADAIAWARAQNGALGPRHEIAHNTFI
                     TAADVPRGRQLGFIWEFSPYIWWPTPITSVDIARAVGPERMRRLWPIREALATGALVI
                     AGSDWPVVPSVNPWLAIETMVSRQAPGATGPAQGAAEAVSRAQALAIFTRNGAAAMGR
                     LDSGGTLEPGKQADMIVIDRNPLTAPLGSLHATRVLATYIAGAQVWAAR"
     gene            complement(5450..6865)
                     /locus_tag="VFF98_09190"
     CDS             complement(5450..6865)
                     /locus_tag="VFF98_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU63846.1"
                     /translation="MRAALPFLSVTLLLGACAGLSGKGRPDGPVPQVAGADGGWIDGA
                     PAGPMAAGWWQALGDPQLDGLIAQGLAASPDVAEAEAKLRSARAALAATRARALPQLD
                     ASGSATTNAQSANGMIPFGRLSQFPGITRSYDLFDAGFDASWEIDIWGARAAATRAAA
                     ARGDMAAAQRDGVRLTLAAEIARTYTQMRADQARRANWEGQEAALAALATLQQARLAA
                     GEATRDEALAATQRLESARAQLAGARGDATADAYALAQLTGQPPEALAGLIGTAAPLP
                     AAPAVALVGLRSDVLARRPDVRGAVADLAAAHADVKAARAALFPSLSLTGSVGQQART
                     TGTFTAADSTRYSLGPSLHWPVFAGGQLRAQLRGARADADAAAARYGKAVLTALADSE
                     TAANRLARAADASAAAASAASAAEATGALADARFIQGEDSRAQALEARLAALSADNAA
                     ISARAAHTAAYVALGKALGAR"
     gene            complement(6870..7997)
                     /locus_tag="VFF98_09195"
     CDS             complement(6870..7997)
                     /locus_tag="VFF98_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63847.1"
                     /translation="MNRWLAFLIRCRAMIIKEMWAVLRDPRARIILVGPPIMQLVLFA
                     SAATLEVKNVEIGVLDLDNGPAAHEVLQQLAGSPNIHALHRLASYADLARAIDRRDVL
                     CAVVLPEGLSRDVAARKAASAGAVCDGRRSNSAQIMGGYLADMVGRAGLALRGVAPDA
                     APGGATVVRHMFNPNLDFLFFVLPALVATVASISSLSVSGQSIARERELGSFDQLMVA
                     PLRLPEILIGKMTPPLIVGLVNATLYLLVITQLFGEPLRGNVFSYYLATFCFLTANIG
                     LGMAVSALAQTQQQAFLGIFLVAVPVNLLSGFAAPVDNMPLMLRMIAEADPQKHMVVL
                     MEGLFLKDMPLATVLANTWPLMVISAVTLTFSSLLFRSRME"
     gene            complement(7994..9112)
                     /locus_tag="VFF98_09200"
     CDS             complement(7994..9112)
                     /locus_tag="VFF98_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63848.1"
                     /translation="MSALFSARRLKAMLVKEGLQILRDPSTILIAFVLPVILILIFGY
                     ALNLDTGRTRIGLAQQDGGEAAASLATAFRQSPWFAVVAVGDVPHLKDELVAGRIRGI
                     IVIPQDFSRRAAAHAGTIQAITDGSLPNNAAFVASYADGVRQNWALARDGRAAVQPAL
                     ALTLRYWFNPGSVSRYFLVPGAIAVVMTMIGSLLTALVLAREWERGTMEAILATPLGM
                     AELVVTKVVPYYVLGLAAMTICTVVSLILFDVPFRGSVLALLAISSCYLFCALGQGLM
                     ISALARSQYVASQIALLAAFLPSLLLSGFIYEISSAPLWVQWVTRIVPARYLIPSLQT
                     VFLAGDDWGLFLPDMARMLLFGAVFFAITLAKTKRRVA"
     gene            complement(9109..10857)
                     /locus_tag="VFF98_09205"
     CDS             complement(9109..10857)
                     /locus_tag="VFF98_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU63849.1"
                     /translation="MSPPAAPLPAAQLPAPLAEARALVKRFGAVTALDGVDMAIVPGE
                     MTGLVGPDGAGKTTLIRILAGLVAPDGGTARVLGQPAVDAPREKLGYMPQRFGLYEDL
                     SVIENLTLYADLRALPAAERADTFARLLEFTDLARFQGRLAGRLSGGMKQKLGLACAL
                     VSRPQVLLLDEPGVGVDPVSRHELWDMVRALAGEGVGVLWSTAYLDEAERCDRVYLLS
                     EGRLLHAGPPQELMGRMEGRVAQIAVPASARRETMRRLRASGAVMDAVRHGDALRLLL
                     ARPLAAADPALGVAPADLIAPRFEDGFIDALGGADLAESALAAQYRTIPESALPAIEA
                     KGLTKRYGAFSAATDIRFSVPRGSIFGLLGPNGAGKSTTFKMLCGLITATEGAGSVAG
                     HDLRRARADARQSLGYMAQKFSLYGDLSVRQNLAFFAGIYGLAGARAREAIDHVSEIF
                     RLGPLMDMNAGTLPLGFRQRLSLGCAVMHQPPVLFLDEPTSGVDPLVRREFWNHITAL
                     VDKGVTVLVTTHFMDEAEYCDRIALIYRSQQIATGTPDELRAAAAGPDGRPVATMEDA
                     FAALIRAADLHREQAA"
     gene            complement(10854..11846)
                     /locus_tag="VFF98_09210"
     CDS             complement(10854..11846)
                     /locus_tag="VFF98_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZU63850.1"
                     /translation="MNRKLMIGGVAGLAVLGLGTAGWQATHPRDRALVLYGNVDVRQV
                     DLGFRVGGRIASLAVDEGQHLAPGAELGRLDARPLQDAADAQNAQLALARANLAKYRA
                     GARPQEIEQARQALTAQQARFEQADAEYRRQQGLAPTGAVSHRQLEAARADRDAARAL
                     LDQARAAYGLQTEGFRREDIAVSAAQADAAAANASKAATDLSDTILRAPEGGTVLTRA
                     REPGAIVQPGETVFTLTIDNPLRLRAYVAETDMARIAPGQAVEVSADGNPKTYHGTIA
                     AIASAAEFTPKTVQTESLRADLVYRVRVLVSDPDGALHQGEPVSVRVTGPAPHR"
     gene            complement(11843..12514)
                     /locus_tag="VFF98_09215"
     CDS             complement(11843..12514)
                     /locus_tag="VFF98_09215"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HZU63851.1"
                     /translation="MDSDKPCARDILLHAALDMFGRRGFDGASTRAIAAAAGMPMSQI
                     TYHFGGKEGLYLGCAQMIAEAMGRIIGSTLVAVEAELAAASAPPMAARAGLRQLLGAM
                     AEAMLDPETRAFSRFMLREQAEPTRAFAIIYGGAMGRVLALLERLIVLISGKDGIAAK
                     VEALALVGQITVFRVAQAGVLEFTGWGGIGPAEVAIIRQTILGNVDVICAALGRSPDE
                     GGRQE"
     gene            complement(12596..15052)
                     /locus_tag="VFF98_09220"
     CDS             complement(12596..15052)
                     /locus_tag="VFF98_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63852.1"
                     /translation="MHMRKTAALALASTALGWAMAAHAEDSARPSPADTDAGQAIVVT
                     GSRGQARTVTSSPTPIDVIGGDQLARLSGGMQLRDALTQLVPSFQAQQVGSSSWDSVA
                     RPAGLRGLSGVDVLVLVNGKRRHNSAMANLNTGNLSPGGNPVDLDQIPASAIKRIEVL
                     RDGAAAQYGSDAIAGVINIILKDAPEGGEASLFAGQRYPDPVNGSDGKTISASVSHGF
                     HLGSRGSIVVSVEGKVQDHTVRNSDATSTIYGKSDPREATANRRIYQGGLPLERQIQF
                     AENASFDTGDLLLYSNATFGYRYAQVGQAGRVPASTSDIIAVYPNGYTPYYTMNETDF
                     QATAGMKGKVRGWDVDLSTAYGRDYVSSGSMNTLNASLGASSPTSFKTFSAAFDQWTT
                     NLDITRGYEVLGGRHLQVSAGAEFRYESYVTKPLDPLSYANGGYVYPTGSTYAGQYAA
                     VGAQGATLVTPSDAANLKRTVWATYLDTALDVTSRWLLTGAVRFEQYDDSSGSNWSGK
                     VSTLYKATGWLNLRAAFSNGFRAPSLTQQGFQSTSDSFTLVGGTYQLIESKTVQPGSA
                     IGQALGATPLKPETSHNTSFGFTLTPLRRLSISVDAYQIDIHNRVALTGYLSGSGVSA
                     ILTANGYSGNEYVRYFTNAINTRTRGLDVVANYNFALGALGDLHASVGYNHNVTNITH
                     IAATPSALSKLGLTLFDRQAQGYFTAFTPKDKIILGLDWQKGRFGVNVKETRYGAWWL
                     MGTTAATDQHYGAKWISDLELSYDLTRRIKMAVGAQNLFNVYPDKNTVANTIGSSIYG
                     SNSPFGAYGGFYYARVQVKL"
     gene            complement(15179..16396)
                     /gene="metC"
                     /locus_tag="VFF98_09225"
     CDS             complement(15179..16396)
                     /gene="metC"
                     /locus_tag="VFF98_09225"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HZU63853.1"
                     /translation="MSETEHPPREQTRMIHPHEGGGQQARTAAPVLQRGTTVLLPACG
                     AFAGPGPTYGRGGLATQTALRAALCDLEAGEGCVLYPSGLAALSGAILALCRAGDEIV
                     MCDTIYAPTRRFVESGLSAYGVSLKLFDPASSPEEIAAGFSERTRLLVMESPGSLTLD
                     MLDVPQLAALARARGVLSLVDNTFAAGVLFKPLEHGVDVSVQALTKYVCGHSDVFMGA
                     AVARGEAAAMLRRSARDVGWTVSSDDAYLALRGLKTLHTRLARHGASALEVARWLADQ
                     PRVAEVLCPALPGARGHALFARDYAGTNGLVTVVLDGPMAATSAFLDSLALFGLGFSW
                     GGFESLAIPCNDQLAQRQTGARWPQPVIRLHVGLEATADLIAELDQALARFEAACAAS
                     DTHTTPARARVAR"
     gene            complement(16551..17042)
                     /locus_tag="VFF98_09230"
     CDS             complement(16551..17042)
                     /locus_tag="VFF98_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU63854.1"
                     /translation="MSRRVGMDGADLRILGALQQDAMLSVAALSERVHLSHNSCWRRL
                     KRLEEAGVIARRVTLLNPQRLGLRLTAYMSVRTSEHSEQWLAGFARAVAAIPAITELY
                     RMTGDVDYLLKLHVASMADYDAVYRKLIALVKLTDVSTAFVMEEIKHTTELPLAVHAP
                     EEG"
     gene            complement(17039..17710)
                     /locus_tag="VFF98_09235"
     CDS             complement(17039..17710)
                     /locus_tag="VFF98_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZU63855.1"
                     /translation="MNWDLREPPPPPPPELVARLAGLDTATLGHWRLWGLCDPAIGAL
                     APGMKACGPAFTLALPGADGTALHHALGQVRPGDVLVIDRLGDGLHAAVGGLVAEAAV
                     QRGVAAIVLDGPVADVAEILTCGLPVWARGRSQRTTRRLGLGGRLNGPVCCGGAVVMP
                     GDLVLADADGVAIVPVEEAPGEVVRAATRAQREADIRAGLQAGRALPDLLPPAPLGMP
                     GRAAR"
     gene            complement(17707..18552)
                     /locus_tag="VFF98_09240"
     CDS             complement(17707..18552)
                     /locus_tag="VFF98_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZU63856.1"
                     /translation="MSAPDPARPGWRRHALADLSHAEFAARLPEAPVIALPLGSHEEQ
                     GPQAAMGDFRLAEEIALMACARADAIAAPVLPFGYAEFFRGMAGGMQLRGPTFRAVLR
                     DMVDSFLDHGLERVVIVNGHSTNAPLIAEVAHAIRRETGVVVPAIHLWRSLPEAVWRA
                     AHGDKAAAARGHGADPVTSVMMHLAPALVRPDRVAPALRQPAFGLPTEAHFSSVSFGG
                     VSVDVPLDVTEVAANGVGQGDAGLASAAAGAVFTRFLVDHVAAFLAHFRRCDPRAPGT
                     PPVAP"
     gene            complement(18549..>19348)
                     /locus_tag="VFF98_09245"
     CDS             complement(18549..>19348)
                     /locus_tag="VFF98_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZU63857.1"
                     /translation="ADKPANLAAIEAAIADAVMRDRPDWLQLPEHCEWLGGSGGAPAV
                     AEAATGGPTWQLLSQAARRHRVWIHGGSFYEQTGVPGRVYNTTVVFDRDGVERARYRK
                     IHMFDVTTADGASYHESATVDRGSAVVSYDCEGVQVGCAICYDLRFAELFQALVARGV
                     DLIALPAAFTLHTGKDHWEVLLRARAIETQCWLAAAAQWGSYPTPAGVRQSFGQSMII
                     DPWGAVVARVSDGLGSAAAPIDLALSARVRAQIPVGANRVFDWQPRL"
BASE COUNT         3132 a         6820 c         6588 g         2808 t
ORIGIN      
        1 gcgcggcatg ccgttcatcc gcacgaacac gctgttcccg acaaaggccg aggtgtagta
       61 atagcggtca gccaggttgc gcacccacag cgccagctgc cacctgtcgc cctctccctt
      121 gatgccgatg cgcccgttga ccagcgtgta gccgggcacc gcagtggccg gatccagcgc
      181 cagcacccgg cccgaggaag acgtgcgcgc gttcatgtcg agcgtgagga agccggtcag
      241 ccggccgttc agcggcgcct cataagtgcc ggccagattg ctctgccagc gcggcgaatt
      301 ggccagccgc gtgccggcca gattggtggc cactgccaga ttggcgccat tgttcacgcc
      361 ggcatcgggc caatcggtca ccaccgcatc gagataggtg gtggaaccat cgaaggagag
      421 ccgcgggctg gcctgccagt gcccctccag ctccaccccc cgcacgcggg agcgcggcac
      481 gttggtcagc tgcaccagcg cgcccacgaa ggtgttgatg taatcggcct cctgcttgtt
      541 gcgatagtca taccagaagg ccgaggcgtt gaggcgcagc gcattgtgca gcaggctgag
      601 cttggcgccc agttcatagg cggtcaccgt ttccgggcga tagggcagca attgcgattg
      661 cagattggtg ttgatgcccg aaaaaccgcc ggatttgaaa ccattggaga tggtgccata
      721 gatcaggctg cggccgatct tcttgtccag ccccactttc cacatggcgc ggttagccgt
      781 gatcgtgtcg ctgaaggggg tgtaggtgcc atagccgggg gtgccggcga tatcgtcata
      841 ggtgccgcag ccgcccggcg tggtgccgac aaaggaagcc agcgtgccat cgccgctgtc
      901 gtaggtgcag ccggtccagc tgcgccgctc atgcgtgtag cgaaagccgc ccagcagccg
      961 ccagccgcca cccagccgat attccgcatg ggcaaagccg gccagcgagc tggtcgtctg
     1021 cgaatagcgc gtgtccagcg tctggatgcc cagcgccagc gcgtaatagc tttgctgcat
     1081 gtagtaattg tagttctcgt gcatcgtatc atgcgagaca tagccgccga cgatccagct
     1141 gagcgggccg ttgccgcgcg aggccaggcg cagttcctcg ctgatgacgg tgagctgggt
     1201 atcgttgatc tggctggaat cattgccggc ccagccatcg ccgtcattcg tctcgcggcg
     1261 ggtgaagcgg tcgtagccgg tgacggaggt gaggctcatg cccggcagat cgaggctggc
     1321 ccgggcgaca aagccggcca gccggttgtt gcgccggggc cggtagatgc catcggacca
     1381 gtctgccgcg gtggcattgc cggtggaaaa ggcggcggtg gggctgccgg cggccgtgct
     1441 ggtgatcacc ggcaggcgca gcgccgactg gccgatcagc tggccgttat aggcggtggc
     1501 cgcctggttg tcggacttgt cgatgatgta atgggccgag acgaacagat cgagcgtggg
     1561 cgtcgccttc cagtcgatcg tgccgcgcac cgccatcaga tccttgttgc ccagcgtgtc
     1621 gcccggccgg ctgatgctgc gctgccaggc gccgccctcg ctcaccttgc cggcgagcct
     1681 cgcggtcagc ccctcggcca gcgggccgct gacaaagcct tccagattcg tctcgccata
     1741 attcgacaca tcgccggcaa aaccggcctg cagcgtggag gttggccggg cggaattgac
     1801 gttgatctgc ccggcggtgg aattgcggcc gtaaaggtcg ccctgcgggc ccttcagcac
     1861 ttccacctgg ccggtatcga agaaggcgcc gcgggtcatc accgcatagg gcatggccac
     1921 ttcatcggca tagatgccca cggtggagga agcgctggtg gaataatcgg aaaagccgat
     1981 gccgcggatc gtgtagatcg gcacgcccga ggcgctggtg gccgtgaccg acaggcccgg
     2041 cgttacctgc gagagcccct cgagcgattg cacgcccttg tcggccaggg tggtgccggc
     2101 aaaggcgctg atggcaatgg gcacatcatt gatgccctgg ctgcgctttt gcgccgtcac
     2161 cacgatttcg ccggcggggc tggcctgtgt gcctgtatct gcgcgcgtat ctgcgcccgt
     2221 atccgcccgg gcgctggccg cgcccagtgc cagcaccagg ccaatggcgg ccgccccctg
     2281 tcgcctgcaa tgccgcttgc acctcatatc cgtcttgcct cctcgcggcc cgatccgaac
     2341 cgcttttgct gttttgggat gcggcaaatc taggaagcgg cggtttggtg tcttgccctt
     2401 gcgtgccaat cggcgttgac tgtgcgtgcc agtggttcag ccacctgccc cgccggcagc
     2461 gttcagccgg gcctggcgcg gcgtgcagcc aaagcgcgcg cgatagctgc gcgagaaatg
     2521 cgccatgtcg ttgaagccca catcgaaggc cagctgggtc agcgatttgg cgcattcctg
     2581 cggcgaggtg aggcgcagat gcgcctgttc cgtgcgcaaa gaccagagcc agctggccgg
     2641 tgtctgcccg gcctcggcaa aggcgcggta gagctggcgg cgcgacacgc ccagccccag
     2701 cgcgatggca tcgggcgaaa gcgcgggatt ggccagctcc ttcagggcaa agcggtgggc
     2761 ccggcgcagc aggccatcgg cggccagatc ctgctcgccg gcgggcgtgg ccgccaccag
     2821 ccggcccagc agctggtgga tcacctcgcc caccactgcc cgctcctcgg cgcaggccgc
     2881 ccggcgctgg gccagcagcg cggccagcgc cgagcccagc accgcgccca ccgccgcctg
     2941 cctgtccacc cgccgcccgc aggccgccag cacctttgcc tcgccgcccg cggcatcgaa
     3001 gggcatcggg atcaccgcca cctcattggc ccggcgcgag gcgaattcgc agccctgata
     3061 ggggttcatc aacaagagat cgcccgcccg cagcaccacc ggcgcgccgc catcgaaatg
     3121 cacgcccagc tggccgctga tctgctgcat gaggaagaac tgcccctccc agcgatcgcc
     3181 cagcccgtgc gcggcgcgct gcgtggtcag cgcgcgggtg tagaccatca ccgggcgcag
     3241 cacatgctgg ccatcctcac gcacgcgaaa ggcggattgc tcgccgatct cgtcggcgct
     3301 gaagcggctg tggccataaa cgccccgcaa ggcgcgctcg aaggattgcg agactgtctc
     3361 tcggcgcaag gtcatcgctg cataactccc catgacgccc tgccgggccc gctgcccggc
     3421 gcgcccctgc cgcccggtta tgccggtgcg gcaggggctt tcgcccgctt gtgttcctcc
     3481 ttggggcgat gcgccgaagg cagcaggccc agcctctcat ggcggccctg cgcagactag
     3541 tcatttccgg ccaatcatgc cctcagcgcg cggcccacac ttgcgcgccg gcgatatagg
     3601 tggccagcac gcgggttgcg tgcaggctgc ccagcggggc ggtcagcggg ttgcggtcga
     3661 tcacgatcat gtcggcctgc ttgcccggct ccagcgtgcc gccgctgtcc agccggccca
     3721 tggcggcagc gccattgcgg gtgaagatcg ccagcgcctg ggcgcgggaa accgcctctg
     3781 cggcgccctg ggcagggccg gtggcgccag gggcctggcg gctcaccatc gtctcgatcg
     3841 ccagccaggg gttgaccgag ggcaccaccg gccagtcgga accggcgatc accagcgcgc
     3901 cggtggccag cgcctcgcgg atgggccaca ggcggcgcat ccgctctggc cccacggcgc
     3961 gggcgatatc caccgaggtg atcggcgttg gccaccagat atagggcgaa aattcccaga
     4021 tgaagcccaa ctggcgccca cgcggcacat cggcggcggt gatgaaggtg ttatgggcga
     4081 tctcgtggcg cgggcccagc gcgccatttt gcgcccgcgc ccaggcgatg gcatcggccg
     4141 ccgcgcgggt ggcgccatct ccggcggcat gcatcttcac ttccagcccc tgggcgtcga
     4201 aagcggcaac ggcggcgttg agccgggcct gcggcacttg cagtatgcca tgcgcgccgc
     4261 ttgtatgggc atagggttcc accatggcgc cggtgtggct ttcggtgggc acgccatcga
     4321 ggaagatctt cacgcaatcg aagcgcatcg gcccggcaga cagttcctgc cggcgcgcga
     4381 tcagcgcctc ggccccgttc gacatcggcc cccacacgat gcagccgcgc gcatgctgct
     4441 tcagcccgcc cgagcgcgca taggccgaca gtgcctgcgc attggccggc cgcaccgcgg
     4501 catcggtgaa gccaacgatg ccataggaca gcatttcggt agtggcggcc tccacggcgg
     4561 ccagcttctc ttcggggctg gcggccggga tatggctttc caccagcgcg gttgccgtct
     4621 cgcgcagcac gcccagcggg gcgccggcgg catcgcggtc gatcacgccg ccggcgggcg
     4681 ccggggtggc cgcggtgatg ccggcgatcg ccagcgcccg gctgttcacc cacaggctgt
     4741 gatgggcctc atcgctcagc gccacgggat ggtcgggcgc ggcctcgtcg agcaggcggg
     4801 cggtctgctc gccggggcgc aaggccgccg ccacccagct gccgcccagg atccacccgc
     4861 ccggccccac ctgccgcgca caggcctgca ccgccgcctt caccgccgcc gcgccgccgc
     4921 cctgcggcag gtggcaggcg cgctcggcaa ggccggcgtc catcacatgc acatgcatgt
     4981 cgtagagccc cggcatcagc gtgcgcccgg ccagatcgac cacttcggcc ccggcatgcg
     5041 gcagggcaag gatctcggca tcgctgccca ccgcaacgat caccccgccg cgcagggcca
     5101 gcgcctgcgc cgtgcccgcc ggcgccagca cctggccgtg caggaagagc cggtctgccg
     5161 gcggggcggt ggtttgcgcc gctgccggca ccaccggcaa cgcctgggcg cacaccggca
     5221 gcgccagggc gcacaccgcc ccccgcaggg cccgatgccc cttgcgccgc cagccgccat
     5281 ccgctgtgcc tgtccccgct gggcctgtcc ccgctgggcc tgtcatcgca gcattcatcg
     5341 ccatgcgctc acctttcgcc tgctcgcccg ccggaagggc gggggctgcg caaggatctg
     5401 gcatggccgc ccgggcagcg tcttgcctga gcgtgccaaa gccggcgggc tagcgcgcgc
     5461 ccagcgcctt gcccagcgcc acataggcgg ccgtgtgcgc cgcgcgcgcg ctgatcgcgg
     5521 cattgtcggc cgaaagggcg gcaaggcggg cttccagcgc ctgcgcgcgg ctgtcctcgc
     5581 cctggatgaa gcgggcatcg gccagggcgc cggtggcctc ggcggcgctg gcggcgctgg
     5641 cggccgcggc gctggcatcg gcggcgcggg ccaggcgatt ggccgccgtc tcgctatcgg
     5701 ccagagcagt cagcaccgcc ttgccatagc gggcagcagc ggcatcggca tcggcccggg
     5761 cgccgcgcag ctgggcgcgc agctggccgc cggcaaacac cggccagtgc aggctgggcc
     5821 ccaggctgta gcgcgtggaa tcggccgcgg tgaaagtgcc ggtagtgcgc gcctgctggc
     5881 ccacgctgcc agtgagcgac aggctgggga acagcgccgc gcgggcggcc ttcacatcgg
     5941 catgggcggc ggccagatcg gccacggcgc cgcgcacatc cggccggcgc gccagcacat
     6001 cggaacgcag ccccaccagc gccaccgccg gcgctgcggg caggggcgct gcggtgccga
     6061 tcagccccgc cagcgcttcg ggtggctggc cggtcaattg cgccagcgca taggcatcgg
     6121 cagtggcatc gccccgggcg ccggccagct gggcgcgggc gctttccagc cgctgagtgg
     6181 cggccagcgc ctcgtcccgc gtggcctcgc cggcggccag gcgcgcctgc tgcagggtgg
     6241 ccagcgccgc cagcgccgct tcctgccctt cccaattggc gcggcgggcc tgatcggcgc
     6301 gcatctgcgt gtaggtgcgg gcaatctcgg cggccagtgt caggcgcacg ccatcgcgct
     6361 gggcggcggc catgtcgccg cgcgcggcgg cagcgcgggt ggcggcggcg cgcgcgcccc
     6421 agatgtcgat ttcccagctg gcatcgaagc cggcgtcgaa caggtcatag ctgcgggtga
     6481 tgccggggaa ctggctgagc cggccgaagg ggatcatgcc attggcgctt tgcgcattgg
     6541 tggtggcgct gcccgaggca tccagctggg gcagcgcgcg cgcgcgggtg gcggcaaggg
     6601 cggcgcgggc ggagcgcagt ttcgcttccg cctcggccac atcggggctg gcggccaggc
     6661 cttgcgcgat cagcccgtcg agctgcggat cgcccagcgc ctgccaccag ccggccgcca
     6721 tggggccggc cggcgcgcca tcgatccagc cgccatcggc gccggccacc tgcggcacgg
     6781 gcccatcggg ccggcctttg cccgagaggc cggcacaggc ccccagcagc agggtgacag
     6841 agagaaaagg caaggcagcg cgcatcgggc tactccatcc gcgagcggaa cagcaaagag
     6901 gaaaaggtga gcgtgaccgc cgagatcacc atcagcggcc aggtgttggc cagcaccgtg
     6961 gccagcggca tgtctttcag gaacaggcct tccatcagca ccaccatgtg cttttgcgga
     7021 tcagcctcgg cgatcatccg cagcatcagc ggcatgttgt ctacaggcgc tgcaaagccc
     7081 gacagcaggt tcaccggcac cgccaccaga aagatgccga gaaaggcctg ctgctgcgtt
     7141 tgcgccagcg ccgacaccgc catgccaagg ccgatattgg ccgtgaggaa gcagaaggtg
     7201 gccagataat agctgaagac attgccccgc aacggctcgc caaacagctg ggtgatgacg
     7261 agcagataga gcgtggcatt gaccaggcca acgatcagcg gcggcgtcat cttgccgatc
     7321 aatatctcgg gcaggcgcaa gggcgccacc atcagctgat cgaacgagcc cagttcccgc
     7381 tcgcgcgcga tcgactggcc cgagaccgac agcgaggaga tcgaggcgac cgttgcgacc
     7441 agcgccggca gcacgaagaa caggaaatcg aggttggggt tgaacatgtg gcgcaccacc
     7501 gtggccccgc cgggcgccgc atcgggcgcc acgccgcgca gcgccagccc ggccctgccc
     7561 accatatcgg ccagatagcc gcccatgatc tgcgccgagt tcgaccgccg cccatcgcac
     7621 accgcccccg cgctggcagc cttgcgcgcg gccacatcgc ggctcaaccc ttcgggcagc
     7681 accaccgcgc acagtacatc gcgccggtct atcgcccggg ccagatcggc ataggaggcc
     7741 aggcggtgca gcgcgtggat attggggctg ccggccagct gctgcagcac ttcatgggcc
     7801 gccgggccat tatcgagatc gagcacgccg atctcgacat tcttcacctc cagcgtggcc
     7861 gccgaggcaa acagcaccag ctgcatgatc ggcggcccca ccagaatgat gcgcgcgcgc
     7921 ggatcgcgca gcaccgccca catctccttg atgatcatcg cccggcagcg gatgaggaag
     7981 gccagccagc ggttcatgcc acgcgccttt tcgtcttggc cagggtgatg gcgaagaaca
     8041 ccgcgccaaa cagcagcatc cgcgccatat cgggcaggaa caggccccag tcgtcgccgg
     8101 ccagaaacac cgtctgcagc gaggggatga gatagcgcgc cggcacgatc cgcgtcaccc
     8161 attgcaccca gaggggcgcg gaggaaatct cgtagatgaa gcccgagagc agcagcgagg
     8221 gcaggaaagc ggccagcagc gcgatctggc tggcgacata ctggctgcgc gccagcgccg
     8281 agatcatcag cccctgcccc agcgcgcaga acagatagca cgaggaaatc gccagcagcg
     8341 ccagtaccga gccgcgaaac ggcacatcga agaggatgag cgagaccacc gtgcagatcg
     8401 tcatcgccgc cagccccagc acataatagg gcaccacctt ggtgacgacc agctcggcca
     8461 tgcccagcgg cgttgccaga atggcctcca tcgtgccgcg ttcccattcg cgcgccagca
     8521 ccagcgccgt cagcagcgag ccgatcatcg tcatcaccac cgcaatcgcc cccggcacga
     8581 ggaaatagcg gctgacagag ccggggttga accagtagcg caaggtgagc gccagggccg
     8641 gctgcaccgc cgcccggcca tcgcgcgcca gcgcccagtt ctgccgcacg ccatcggcat
     8701 aggaagcgac aaaggcggca ttgttgggca gcgaaccatc ggtgatcgcc tggatggtgc
     8761 cggcatgggc ggcggccctg cggctgaaat cctgcgggat gacgatgatg ccgcggatgc
     8821 gcccggccac cagctcgtcc ttcagatgcg gcacatcgcc caccgccacc acggcaaacc
     8881 agggcgactg gcgaaaggcg gtggccaggc tggcggccgc ctcgccgcca tcctgctggg
     8941 ccaggccaat gcgcgtgcgc ccggtatcga ggttgagcgc atagccgaag atgaggatga
     9001 ggatcaccgg cagcacaaag gcgatgagga tggtggaagg atcgcgcagg atctgcagcc
     9061 cttccttcac cagcatcgcc ttcaggcgcc gggcagagaa gagcgcgctc atgccgcctg
     9121 ctcccggtgc aggtctgccg cgcggatgag ggcggcaaag gcgtcttcca tggtcgccac
     9181 cgggcggcca tcggggccgg cggcagcggc gcgcaattcg tcgggcgtgc cggtggcgat
     9241 ctgctgcgaa cgatagatca gggcgatgcg gtcgcaatat tccgcctcgt ccatgaaatg
     9301 ggtggtcacc agcacggtca cgcccttgtc gaccagcgcg gtgatatggt tccagaattc
     9361 gcgccgcacc agcggatcga cacccgaggt cggctcatcg aggaacagca ccggcggctg
     9421 gtgcatcacc gcgcagccca gcgacaggcg ctggcgaaag cccagcggca gcgtgccggc
     9481 gttcatgtcc atcagcgggc cgaggcggaa gatctcgctc acatggtcga tcgcctcgcg
     9541 cgcgcgggcg ccggcaaggc catagatgcc ggcgaagaaa gccaggttct ggcgcaccga
     9601 cagatcgcca tagagcgaga atttctgcgc catatagccc agcgactggc gcgcatcggc
     9661 gcgtgcgcgg cgcagatcat gcccggccac gctgcccgcc ccttccgtgg cggtgatgag
     9721 gccgcacagc atcttgaagg tggtggattt gcccgcgcca ttggggccca gcaggccaaa
     9781 gatgctgccg cgcggcacgg aaaagcggat atcggtggcc gcggaaaagg cgccatagcg
     9841 cttggtcagc cccttcgcct cgatcgccgg caaagcgctt tcgggaatgg tgcgatattg
     9901 cgcggccagc gcgctttcgg ccagatccgc gccgcccagc gcatcgatga agccatcctc
     9961 gaaacgcggc gcgatgagat cggccggcgc cacccccagc gccggatccg ccgccgccag
    10021 cggccgggcc agcagcaggc gcagcgcatc gccatggcgc accgcatcca tcaccgcccc
    10081 gctggcgcgc aggcggcgca tcgtctcgcg ccttgcgctg gccggcacgg caatctgggc
    10141 cacgcgcccc tccatgcggc ccatcagctc ctgcggcggg ccggcatgca gcaggcggcc
    10201 ctcgctcagc agataaaccc ggtcgcagcg ctcggcctcg tcgagatagg cggtggacca
    10261 caagaccccc accccctcgc ccgccagcgc gcgcaccata tcccacaatt catgccgcga
    10321 gacaggatcc acccccaccc ccggctcgtc cagcagcagc acttgcgggc gcgacaccag
    10381 cgcgcaggcc agccccagct tctgcttcat gccgcccgac aggcgcccgg ccagccgccc
    10441 ctgaaaacgc gccagatcgg tgaattccag caggcgggcg aaggtgtcgg cccgctctgc
    10501 cgccggcagc gcgcgcagat cggcataaag cgtgaggttc tcgatcaccg agagatcctc
    10561 atacaggcca aagcgctgcg gcatatagcc cagcttctcg cgcggggcat caacggccgg
    10621 ctggcccagc acccgcgccg tgccgccatc gggcgcgaca aggccggcaa ggatgcggat
    10681 cagcgtcgtc ttgccggcgc catcagggcc gacaaggccg gtcatctcgc ccggcacgat
    10741 ggccatgtcc acaccgtcca gcgcggtcac cgcgccaaag cgcttcacca gcgcgcgcgc
    10801 ctcggccagc ggggccggca actgggcggc cggcaggggg gcggcaggcg ggctcaccgg
    10861 tgcggcgcag ggccggtcac gcgcacgctc accggttcgc cctgatgcag cgcgccatca
    10921 gggtcgctca ccagcacccg cacgcgatag acaagatcgg cccgcaggct ttcggtctgc
    10981 accgtcttgg gagtgaattc ggcggcgctg gcgatggcgg cgatggtgcc gtgataggtc
    11041 ttggggttgc catcggcgct cacttccacc gcctggcccg gcgcgatgcg agccatatcg
    11101 gtttccgcca cataggcgcg caggcgcagc ggattgtcga tggtgagagt gaacaccgtc
    11161 tcgccgggct ggacgatcgc ccccggctca cgcgcgcggg taagcacggt gcccccttcc
    11221 ggcgcacgca ggatggtgtc ggaaaggtca gtggcggcct tgctggcatt ggcggcggcg
    11281 gcatcggcct gggcggccga aacggcgata tcctcgcggc ggaagccttc tgtctgcagg
    11341 ccataggccg cgcgggcctg atcgagcagc gcgcgcgcgg catcgcggtc ggcccgcgcc
    11401 gcttccagct ggcggtggct gacagcgcct gtgggggcaa gcccctgctg gcggcgatat
    11461 tccgcatcgg cctgctcgaa acgcgcctgc tgggcggtca gcgcctggcg cgcctgctcg
    11521 atctcctgcg ggcgggcgcc ggcgcggtat ttggcgagat tggccctggc cagcgccaac
    11581 tgggcgttct gggcatcggc cgcatcctgc agggggcgcg catccagccg gcccagctcg
    11641 gcgccggggg ccagatgctg gccctcgtcc accgccagcg aagcgatccg cccgcccacg
    11701 cgaaagccca gatccacctg ccgcacatcg acattgccat agagcaccag cgccctgtcg
    11761 cgcgggtggg tggcttgcca gccggccgtt cccagcccca gcacggccaa tccggccacg
    11821 ccgccgatca tcagcttgcg gttcattcct gcctgcctcc ttcgtcaggt gatcggccca
    11881 gcgccgcgca gatcacatcg acattgccga gaatggtttg ccggatgatg gcaacctcgg
    11941 ccggcccgat gccgccccat ccggtgaatt ccagaacgcc cgcctgggcc acgcgaaaaa
    12001 cggtgatctg gccgaccagc gccagagcct cgacctttgc ggcgatcccg tccttgcccg
    12061 aaatcaacac gatgagccgt tccagcaaag ccagcacgcg ccccatcgcc ccgccataga
    12121 tgatggcaaa ggcgcgggtg ggctcggcct gctcgcgcag catgaagcgg gagaaggcgc
    12181 gggtttcagg atcgagcatg gcctcggcca tggcgcccag cagctggcgc aggcccgccc
    12241 gcgcggccat cggcggggcg ctggcggccg ccagttcggc ttccaccgcc accagcgtgc
    12301 tgccgatgat gcgccccatc gcctcggcga tcatctgcgc gcagcccaga tagaggcctt
    12361 ccttgccgcc gaaatgatag gtgatctgcg acatcggcat gccggctgcc gccgcgatcg
    12421 cccgggtgga cgcgccatcg aacccccgcc gcccgaacat gtcgagcgcg gcgtgcagca
    12481 aaatatcgcg ggcacagggc ttgtcgctgt ccatggcgcg cgagattaat tcaaacgaac
    12541 gaacagggca agagggaccg gcccgcgccc cggctggcgc gggccggccg ccggcttaca
    12601 gcttcacctg cacccgggca taatagaagc cgccataagc gccgaagggc gaattgctgc
    12661 catagatcga cgagccgatg gtgttggcga cagtgttctt gtcggggtag acgttgaaca
    12721 ggttctgcgc gcccaccgcc atcttgatgc ggcgggtgag atcatacgac agttccagat
    12781 cgctgatcca cttggcgcca taatgctggt cggtggcagc agtcgtgccc atcagccacc
    12841 aggcgccata gcgcgtttcc ttcacattga cgccaaagcg ccccttctgc caatccagcc
    12901 cgaggatgat cttgtccttg ggggtgaagg cagtgaaata gccctgcgcc tggcggtcga
    12961 acagggtgag gcccagcttc gagagcgcgg acggcgtggc cgcgatatgg gtgatgttgg
    13021 tcacattgtg gttatagccc accgaggcgt gcagatcgcc cagcgccccc agcgcgaaat
    13081 tgtaattcgc caccacatcg aggccgcggg tgcgggtgtt gatggcattg gtgaaatagc
    13141 ggacatattc gttgcccgaa tagccgttgg cggtgagaat ggcgctgacc ccgctgcccg
    13201 acagatagcc ggtcagcgcc acgcggttgt ggatgtcgat ctgataggca tcgaccgaga
    13261 tgctgaggcg gcgcagcggc gtgagggtga agccgaagct ggtgttatgc gaggtttccg
    13321 gcttcagcgg cgtggcgccc agcgcctggc cgatggcaga gccgggctgc acggtcttcg
    13381 attcgatcag ctgataggtg ccgcccacca gggtgaagga atccgaggtg gactggaacc
    13441 cctgctgggt aagcgagggc gcccggaaac cgttggagaa ggccgcccgc aggttcagcc
    13501 agccggtcgc cttgtaaagc gtggagacct tgccgctcca gttgctgccc gagctgtcgt
    13561 catattgctc gaagcgcaca gcgcccgtca gcagccagcg gctcgtcaca tcaagggctg
    13621 tgtcgagata ggtggcccac accgtgcgct tgagattggc cgcatccgac ggcgtgacca
    13681 gcgttgcccc ctgcgcgccc accgcggcat actggccggc ataggtgctg ccggtgggat
    13741 agacatagcc gccattggca taggagagcg gatcaagcgg cttggtgaca tagctttcgt
    13801 agcggaattc ggcgcccgcc gagacctgca gatgccgccc gcccagcact tcatagccgc
    13861 gggtgatatc gaggttggtg gtccactggt cgaaggcggc cgagaaggtc ttgaagctgg
    13921 tcgggctgct ggcccccagc gaggcgttca gcgtgttcat cgagccggag ctgacataat
    13981 cgcggccata ggcggtgctc aagtccacat cccagccgcg caccttgccc ttcatgccgg
    14041 cggtggcctg gaaatccgtc tcgttcatcg tgtaataggg ggtgtagcca ttggggtaga
    14101 cggcgatgat atcgctggtg ctggccggca cgcggcccgc ctggcccacc tgcgcatagc
    14161 gatagccgaa agtggcgttg ctgtagagca gcaggtcgcc ggtatcgaag ctggcgtttt
    14221 cggcgaactg gatctggcgc tccagcggca ggccgccctg atagatgcgg cggttggcgg
    14281 tcgcctcgcg cgggtcgctc ttgccataga tggtgctcgt cgcatcgctg ttgcgcacgg
    14341 tgtgatcctg caccttgccc tccaccgaga cgacgatcga gccccggctg cccagatgga
    14401 agccatgcga gacagaagcg gagatcgtct tgccatccga gccgttcacc ggatcgggat
    14461 agcgctggcc ggcaaacagg ctggcctcgc caccctcggg cgcatccttg aggatgatgt
    14521 tgatgacgcc ggcaatcgca tcagagccat attgcgccgc cgcgccatcg cgcagcacct
    14581 cgatgcgctt gatcgcgctg gccgggatct gatcgagatc gaccggattg ccgccgggcg
    14641 acagattgcc ggtgttgagg ttggccatcg ccgaattgtg ccgccgcttg ccgttcacca
    14701 gcaccagcac atccacaccc gaaaggccgc gcaggcccgc cggccgcgcc acgctatccc
    14761 acgaggagga gcccacctgc tgcgcctgga aggagggcac cagctgggtg agcgcatcgc
    14821 gcagctgcat gccgcccgac aggcgcgcca gctgatcgcc gccgatcaca tcgatcgggg
    14881 tggggctgga ggttacggtg cgcgcctgcc cgcgcgatcc ggtaacgacg atcgcctggc
    14941 cagcatccgt atccgcaggc gaaggcctgg cgctatcctc ggcatgggcg gccatggccc
    15001 agccaagggc ggtggacgcc agcgccagcg cggcagtctt tctcatatgc atgtcagtcc
    15061 cctttgcgac cccctgaacg ggtctgaacg tgtgatccgg cccttgcagg catcctctcc
    15121 cgccggccgg ctggcgcggg gcggatctgt gcccgcctgc cgcgccgccg gccttgttct
    15181 accgtgcgac ccgggctctt gccggtgtgg tatgggtgtc agacgcggcg caggccgcct
    15241 cgaagcgggc cagcgcctga tcgagttcgg cgatgagatc ggcggtggct tccagcccca
    15301 catgcaggcg gatcaccggc tgcggccagc gcgcgccggt ctggcgctgg gccagctgat
    15361 cgttgcaggg gatcgccagg ctttcaaaac cgccccagct gaagcccagg ccaaacagcg
    15421 ccaaactgtc gagaaaggca gaggtggctg ccatcggccc atccagcacc accgtcacca
    15481 gcccgttggt gccggcataa tcgcgggcaa acagcgcatg gccgcgcgcg ccgggcagcg
    15541 ccgggcacag cacttcggcc acgcgcggct gatcggccag ccagcgcgcc acctccagcg
    15601 cgctggcgcc atggcgagca aggcgcgtgt gcagcgtctt cagcccgcgc agggccagat
    15661 aggcatcgtc ggaagagacc gtccagccga catcgcgggc agagcgccgc agcatagccg
    15721 ccgcctcgcc gcgcgccacc gccgcgccca tgaacacatc ggaatggccg cagacatatt
    15781 tggtgagcgc ctgcacgctg acatccacgc catgttccag cggcttgaac agcacgccgg
    15841 cggcaaaggt gttgtccacc aggctgagca cgccgcgcgc ccgcgccagg gcggccagct
    15901 gcggcacatc cagcatgtcg agcgtgagcg agcccgggct ttccatcacc agcaagcggg
    15961 tgcgttcgga aaagccggcg gcgatctctt ccgggctgct ggccggatca aacagcttca
    16021 ggctgacccc ataggccgac aggcccgatt ccacgaaacg gcgcgtgggc gcatagatcg
    16081 tgtcgcacat gacgatctcg tcgcccgcgc ggcacagcgc cagaatggcc cccgacagcg
    16141 ccgccagccc ggatggatag agcacgcagc cctcgcccgc ttccagatcg cacagcgccg
    16201 cgcgcagggc cgtttgcgtg gcaaggccgc cgcggccata ggtggggccg ggcccggcaa
    16261 aggcgccgca ggccggcagc agcacggtgg tgccgcgctg cagcacgggg gcggccgtgc
    16321 gcgcctgctg cccgccgccc tcatgcgggt ggatcatgcg ggtctgctcg cgcggggggt
    16381 gctctgtctc tgacacggaa aatgggtcct tcatgatgtt gcgggccggc agcggccact
    16441 cccgtcggcc taggcggcgc ggcggtggcg cgtctcaccc cgtttgcgcg cgggatcgcg
    16501 gtttttcggg atgttcatcc cgcttttcgc gcctgtgcgg gggatggcca tcacccttcc
    16561 tccggcgcat gcacggccag cggcaattcg gtggtgtgct tgatctcttc catcacgaaa
    16621 gcggtactca cgtcggtcag cttcaccagc gcgatcagct tgcgatagac ggcatcgtaa
    16681 tcggccatgc tggccacatg cagcttgagc aggtagtcca catcgccggt catccggtaa
    16741 agctcggtga tcgccgggat cgccgccacg gcccgggcaa agccggccag ccattgttcc
    16801 gaatgctcgc tggtgcgcac gctcatatag gcggtcagcc gcaggcccag ccgctggggg
    16861 ttcagcaggg tgacccggcg ggcgatcacg ccggcttcct ctagccgctt cagccgccgc
    16921 cagcagctgt tgtgcgagag atgcacccgc tccgacagcg ccgcgacgga cagcatcgca
    16981 tcctgctgca aggcgcccag aatgcgcagg tccgccccgt ccatgcccac ccggcggctc
    17041 atcgggcggc cctgcccggc atgcccagcg gcgccggcgg cagcagatcg ggcagcgccc
    17101 ggccggcctg cagcccggcg cggatatcgg cctcgcgctg ggcgcgggtg gccgcgcgca
    17161 ccacctcgcc cggcgcctcc tccactggca cgatcgccac cccatcggca tcggccagca
    17221 ccagatcgcc cggcatcacc accgctccgc cgcagcacac cggcccgttc agccgcccgc
    17281 ccaaccccag ccggcgcgtg gtgcgctggc tgcgcccgcg cgcccacacc ggcaggccgc
    17341 aggtaaggat ctcggccaca tcggccaccg gcccatccag cacgatggcc gccacgccgc
    17401 gctgcaccgc cgcttctgcc accagcccgc ccacggccgc atgcaggcca tcgcccagcc
    17461 ggtcgatcac cagcacatcg cccggccgca cctgccccag cgcatggtgc agcgccgtgc
    17521 catcggcgcc cggcagggcc agcgtgaagg ccggcccgca cgccttcatc cccggcgcca
    17581 gcgcgccgat cgccggatcg cacaggcccc acagccgcca gtggcccagc gttgccgtat
    17641 ccagcccggc cagccgcgcc accagttcgg gcggcggcgg cggcggcggt tcgcgcaggt
    17701 cccagttcat ggcgccaccg gcggtgtgcc cggcgcgcgc ggatcgcagc ggcggaaatg
    17761 cgccagaaag gcggccacat gatcgacgag gaagcgcgtg aacaccgccc ccgccgccgc
    17821 cgatgccagc cccgcatcgc cctggcccac gccattggcg gccacttccg tcacatccag
    17881 cggcacatcc accgacacgc cgccaaagct gaccgaggaa aaatgcgctt ccgttggcag
    17941 gccaaaggcg ggctggcgca gggccggcgc caccctgtcg ggccgcacga gggcgggcgc
    18001 cagatgcatc atcaccgagg ttaccggatc ggcgccatgg ccgcgcgcgg cggcggcctt
    18061 gtcgccatgg gccgcgcgcc acacagcctc gggcagcgag cgccacaaat ggatcgccgg
    18121 caccaccacc ccggtctcgc ggcggatggc atgggccact tcggcgatca gcggggcgtt
    18181 ggtggaatgg ccgttgacga tgaccacccg ctccagccca tgatcgagga aactgtccac
    18241 catgtcgcgc agcaccgcgc ggaaggtggg gccgcgcagc tgcatgccgc cggccatgcc
    18301 gcggaaaaac tccgcatagc caaagggcag caccggcgcg gcaatggcat cggccctggc
    18361 gcaggccatc agggcaatct cctcggccag gcggaaatcg cccatggcgg cctgggggcc
    18421 ctgctcctca tggctgccca gcggcagcgc gatcaccggc gcctcgggca ggcgggcggc
    18481 aaattcggcg tggctgagat cggccagcgc atggcggcgc cagcccggcc gggcgggatc
    18541 gggcgcgctc acaggcgggg ctgccagtcg aacacgcggt tggcgcccac cgggatctgc
    18601 gcgcgcaccc gggccgacag cgccagatcg atgggcgcgg ccgcgctgcc cagcccatcg
    18661 gacacgcgcg ccaccaccgc cccccaggga tcgatgatca tcgactggcc gaaggactgg
    18721 cgcacgcccg caggcgtggg atagctgccc cattgcgccg ccgctgccag ccagcactgc
    18781 gtctcgatgg cgcgggcgcg cagcagcact tcccagtgat ccttgccggt gtgcagcgtg
    18841 aaggcggccg gcagcgcgat gagatccacc ccgcgcgcca ccagcgcctg gaacaattcg
    18901 gcaaagcgca ggtcatagca gatggcgcag cccacctgca cgccctcgca atcatagctg
    18961 acgacggcag agccgcggtc caccgtggct gattcgtgat agctggcgcc atcggccgtg
    19021 gtcacgtcga acatgtggat cttgcggtag cgggcgcgct ccaccccgtc gcggtcgaac
    19081 accacggtgg tgttgtagac gcggccggga acgcccgtct gttcgtagaa gctgccgccg
    19141 tggatccaca cgcgatggcg gcgcgcggcc tgcgagagca gctgccaggt ggggccgccc
    19201 gtggccgctt ccgccaccgc cggcgccccg ccagagccgc ccagccattc gcaatgttcg
    19261 ggcaattgca gccagtcggg ccggtcgcgc atcaccgcat cggcaatggc ggcctcgatc
    19321 gcggcgagat tggcgggttt gtcggccc
//
LOCUS       DASRYE010000178         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_204795,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000178 DASRYE010000000
VERSION     DASRYE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204795"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFF98_09250"
     CDS             complement(<1..968)
                     /locus_tag="VFF98_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU63858.1"
                     /translation="MQFHANGFRSGDPDVQPASAEGAARQAATCPPAQVDVLIVGCGP
                     AGLTLGAQLAAFPDISLCIVDQAEGPLELGKADGVACRSMEMFNAFGFAERVLREAYW
                     VNETTFWKAGDGQGAGIVRSGRVHDTEPGLSEFPHVILNQARVHGFYLDIMRNAPNRT
                     EPWYGRKLVGLETDPVATHPVTAHFEVTGADGQTRVETVRARYAVGCDGARSAVRGAL
                     GLALKGDSANQAWGVMDVLAVTDFPDIRLKSLITSVNGGTIVLIPREGGYLVRIYVEM
                     DKLGAGERVAARHFGVEEIVAAANKVLHPYTLEVKEVAWWSVYEIG"
     gene            1116..1580
                     /locus_tag="VFF98_09255"
     CDS             1116..1580
                     /locus_tag="VFF98_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU63859.1"
                     /translation="MSNQDNMPGHLARRFQQMAVAMFHTHVDPAGHDLTPVQYSALAA
                     VLAQPGIDQATLAGRIGIDRATITGVIDRLARKGLLTRTVSKADRRARLLQMTDTGTA
                     VLQQIQPAVDAAQDEMVAQLSQEERDLLLALLRKAMGGREAQAAPPGQPAHP"
     gene            1914..4136
                     /locus_tag="VFF98_09260"
     CDS             1914..4136
                     /locus_tag="VFF98_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63860.1"
                     /translation="MALTLALATGGTPALAADDSANGAPSANAQIQEIVVTAQKRSEN
                     LQKASISINAVRGEDLAKSGVHDAAGLQDLVPNVKFSAADELTVSIRGLGTTNINGGV
                     DSAVAYSEDGLYLSHPAALSPLLFDMQRVEVLLGPQGTLYGRNSNAGVVNFISADPEF
                     RTGGHAQIGYGNYNALNSELAVNLPLSDKLALRIAGASEQHGPYVADGTNDSESYAAR
                     MKLLYKPTDTLSILLAATYSAHRTTGTSYGWKCPPGLAGVPACAGTNTPAWSGIYPAN
                     SPLQGMRDHVWGVSATVNLEMPWANFTSLTGYKYYNYHSVNSPGLVNGTDEFDYHHLE
                     SDKFFTQELRLTSKPGSHIKWSAGLYYSDDTQPYSQIFQYNSSTAVQLAGGPDNFYQQ
                     FAFTGTSYKSAAAFGDLTVPVRHDVRLRAGLRYTVESKDATGQISQGITGYGQLGDTS
                     YNPSHEAAWRLTWSAGLDWDISPRNLFYVTASNGYKSGGLNSMPASTGLTTYGPEKIT
                     AVEIGLKNRFLGNRLQVNMNLFHYAYDGYQALIFYMPTGGPLLGDTLFPTVNSQTATF
                     EGGEVQAIWKLTANDRLSFGFNYLHDRFGRFVVALPYAATQDLSGSRVPQAPETTVTI
                     GLDHTFHLPGDMALNLGASTQFVSSYLAQGNYTQYSDAGAAIGTGYYTQGAFHKTSLT
                     ATLTAHGGWTLSAFARNIENKATINTIAGGYPIAQNILQANAMVDPPRTFGFALRKDF
                     "
BASE COUNT          805 a         1475 c         1223 g          679 t
ORIGIN      
        1 tggccgatct catagaccga ccaccacgcc acttccttca cctccagcgt gtagggatgc
       61 agcaccttgt tggccgccgc gacgatctcc tccacgccga agtggcgggc ggccacgcgc
      121 tcgcccgcgc ccagcttgtc catctccaca tagatgcgca ccagatagcc gccctcgcgc
      181 gggatcagca cgatggtgcc gccattgacc gaggtgatca gcgacttgag gcggatatcg
      241 ggaaaatcgg tgacggccag cacatccatc acgccccagg cctggttggc cgaatcgccc
      301 ttcagcgcca gccccaacgc gccgcgcacc gcactgcgcg cgccatcgca gcccaccgca
      361 tagcgcgccc gcacggtttc cacgcgggtc tgcccatcgg cgccggtcac ttcgaaatgc
      421 gccgtcaccg ggtgcgttgc gaccgggtcc gtctccagcc ccaccagctt gcggccatac
      481 cacggctcgg tgcggttggg cgcgttgcgc atgatatcga ggtagaagcc atgcacgcgc
      541 gcctggttca ggataacatg cggaaattcc gaaaggccgg gctctgtgtc atgcacccgg
      601 ccgctgcgca cgatgcctgc gccctgcccg tcgccggctt tccagaaggt cgtctcgttc
      661 acccaatagg cttcgcgcag caccctttcg gcaaaaccga aggcgttgaa catctccatc
      721 gagcggcagg ccacgccatc ggccttgccc agctccagcg gcccctcggc ctgatcgacg
      781 atgcacaggc tgatatcggg gaaggcggcc agctgcgcgc ccagcgtaag gccggccggg
      841 ccgcagccca cgatcagcac atccacctgt gcgggcgggc aagtggcggc ctggcgcgcc
      901 gcgccctcgg ccgaagcggg ctgcacatcc ggatcgccgg aacggaaacc attggcatgg
      961 aattgcattg cgtctctctc ccagggttga aaacacccct ctcttgcgca tggctctgcc
     1021 cgcattagga caggcttggc gaattggccg gggtgatata attcgtgtac tgatgattcg
     1081 tacacatgtc aatttgagaa agcgaggctt cccacgtgtc caaccaggac aacatgcccg
     1141 gccatctggc gcggcgtttc cagcagatgg ccgtggccat gttccacacc catgtagacc
     1201 cggccggcca cgatctcacc ccggtgcaat attccgcgct ggccgccgtg ctggcccagc
     1261 cggggatcga tcaggcaacg ctggccggcc gcatcggcat agaccgcgcc acgatcaccg
     1321 gggtgatcga ccggctggcc cgcaagggac tgctcacccg gaccgtgagc aaggccgacc
     1381 ggcgcgcgcg cctgctgcag atgaccgaca ccggcacagc cgtgctgcag cagatccagc
     1441 cggccgtgga tgccgcacaa gacgagatgg tggcgcagtt gagccaggag gagcgcgacc
     1501 tgctgctggc cctgctgcgc aaggcaatgg gcgggcgcga ggcccaggcc gctccgcccg
     1561 gccaacccgc tcacccttga ttattactca aaggtgcccc caaaaattgc attgaccgat
     1621 acacccccca actgggatgc gggcatcagg gacacagcgg aaaaccgcag gatcaaaaag
     1681 gagagagcgg ccccctgcca ctcgggaagg gcccggttat cggcgtaagc gccaatggaa
     1741 taccgacaga tcggccatgg ccggccgcct ttgcgcggca ggatgcggcg cgccatgccg
     1801 gcgcgaatgc cgcccctaat gctggcccaa tgctgccttg cctataaatc acaattcgga
     1861 ggagagccat gaagcgcaca tatcccagga ccacagccgg caggagcagc ctgatggccc
     1921 tgacgctggc ccttgccacc ggcggcaccc ccgcccttgc cgcagacgac agcgccaatg
     1981 gcgcccccag cgccaacgcg cagatccagg aaatcgtcgt caccgcgcag aagcgttcgg
     2041 aaaatctgca gaaggcctcc atctccatca atgcggtcag gggcgaggat ctcgccaaat
     2101 cgggcgtgca tgatgcagcg ggcctgcagg atctggtgcc caacgtgaaa ttttccgccg
     2161 ccgacgagct gaccgtcagc atccgcgggc ttggcaccac caatatcaac ggcggcgtcg
     2221 attcggccgt ggcctacagc gaggatggcc tctacctgtc gcatccggcc gcgctctcgc
     2281 cgctgctgtt cgacatgcag cgggtggaag tgctgctggg gccgcagggc acgctgtatg
     2341 gccgcaattc caatgccggc gtggtcaatt tcatcagcgc cgatccggaa tttcgcaccg
     2401 gcggccatgc ccagatcggc tatggcaact acaatgcgct gaattcggaa ctggcggtca
     2461 acctgccgct gtcagacaag ctggccctgc gcattgccgg cgccagcgaa cagcacgggc
     2521 cctatgtggc ggatggcacg aatgattccg aatcctatgc cgcccgcatg aagctgctct
     2581 acaagcccac cgacacgctg agcatcctgc tggccgccac ctattccgcc catcgcacca
     2641 ccggcacctc ctatggctgg aaatgcccgc ccggccttgc cggcgtgccc gcctgcgccg
     2701 gcaccaacac ccccgcctgg agcggcatct atccggccaa cagccccttg cagggcatgc
     2761 gcgatcatgt ctggggggtg agcgccacgg tcaatctgga aatgccctgg gcgaatttca
     2821 cctcgcttac cggctacaaa tactacaact accactcggt caattcgccc ggcctggtga
     2881 atggcacgga tgagttcgat taccaccacc tggaatcgga caaattcttc acccaggaac
     2941 tgcgcctgac cagcaagccc ggatcgcaca tcaaatggag cgccggcctc tattattccg
     3001 acgacaccca gccctacagc cagatcttcc aatacaacag cagcaccgcc gtacagctgg
     3061 ccggcgggcc ggataatttc taccagcagt tcgccttcac cggcacctcg tataaatcgg
     3121 cggcggcctt tggcgatctt accgtgccgg tgcgccatga tgtgcgcctg cgcgcagggc
     3181 tgcgctacac ggtggaatcc aaggatgcca ccggccagat cagccagggc atcaccggct
     3241 atggccagct gggcgacacc agctacaacc cctcgcacga ggccgcctgg cgcctcacct
     3301 ggagcgccgg cctcgactgg gacatcagcc cgcgcaacct cttctatgtc accgccagca
     3361 atggctacaa atcgggcggg ctcaattcca tgcccgcttc caccggcctt accacctatg
     3421 ggccggaaaa aatcaccgcg gtggaaatcg gcctgaagaa ccgcttcctg ggcaaccgcc
     3481 tgcaagtgaa catgaacctg ttccactatg cctacgacgg ctatcaggcg ctgatcttct
     3541 acatgcccac cggcgggcca ctgctgggcg acacgctgtt ccccacggtc aattcgcaaa
     3601 ccgccacctt cgagggcggc gaggtgcagg cgatctggaa gctgaccgcc aacgaccggc
     3661 tgagcttcgg cttcaactat ctgcatgatc gcttcggccg gtttgtcgtg gcgctgccct
     3721 atgcggccac gcaggatctg tcgggcagcc gcgtgccgca ggcgccggaa accacggtga
     3781 cgatcggcct cgatcacacc ttccacctgc ccggcgacat ggcgctgaac ctgggcgcca
     3841 gcacgcaatt cgtctccagc tatctggcgc agggcaatta cacgcaatat tccgatgccg
     3901 gcgctgccat cggcaccggc tattacacgc aaggcgcctt ccacaagacc agcctcaccg
     3961 ccacgctgac cgcccacggc ggctggacgc tgagcgcctt tgcccgcaac atcgagaaca
     4021 aggccaccat caacaccatc gccggcggct atcccatcgc ccagaacatc ctgcaggcca
     4081 atgccatggt cgatccgccg cgcactttcg gctttgccct gcgcaaggat ttctgagccc
     4141 cggggcttgg gggactttgg ggggcgcgag gccaggcctt gc
//
LOCUS       DASRYE010000179        24273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_59924,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000179 DASRYE010000000
VERSION     DASRYE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 24273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24273
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59924"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..547)
                     /gene="secB"
                     /locus_tag="VFF98_09265"
     CDS             complement(32..547)
                     /gene="secB"
                     /locus_tag="VFF98_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZU63861.1"
                     /translation="MADEGNVTSNLNLDADTLPAAGVISQYVKDLSVENPNAPDCYAW
                     ADTPEMDVQFNIAAHPLQGEVHEVELKIKLTAKAEAGVAYLVDLTYAGLVGMRNLDEA
                     NMHAFMFAEAPRILFPFARRVVADAVRDAGFQPLLLEPIDFNGIYLQQLAAQAQQADG
                     ADALGEPAGHA"
     gene            874..1542
                     /locus_tag="VFF98_09270"
     CDS             874..1542
                     /locus_tag="VFF98_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HZU63862.1"
                     /translation="MTVEIVILAMIAALLGLRLYSVLGRRAEHGEEPLQSRQAGQQAQ
                     PATAPARLPERALPQQSAPRAREIPAVVPAVEKALREIMGADRRFDPNAFVDGARQAY
                     RMILDAFWRGDKDELAHLCDPVVAGRFGEAIDARKTAGEVPDNRVVRINEAAIVGAAL
                     AGTTARVAIRFVTDIAAVTRDGEGRVIAGSLDDAVEVRDVWTFCRDIRATDPDWLLDE
                     TDEG"
     gene            1549..2796
                     /locus_tag="VFF98_09275"
     CDS             1549..2796
                     /locus_tag="VFF98_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HZU63863.1"
                     /translation="MFKTIAGPRWLVLAAMLSLAGCGRIIPEGHPPGALPPPGPAAPG
                     QPYAPAPLPPNAVALGVHRGPDPVSLGLTAANAAPALAAFREACGHLTGRADPSGLTQ
                     PIDWQAPCGAAKSWRADEADRFFTTFFESVVVGEGNANVTGYYEPEISGVKTRQPGFD
                     VPIYGLPGDLVHARPGDAQVKPNGQTPFGRYDELGHFSPYYERAEIENGMLANRGLEI
                     GWAADPVELFFLQIQGSGRLRAPDGSITRIGYAADNGRDYTSIGIILRDRGLIGSGPG
                     QYPGSMQGIMQYLRDHPYEGRQLMDMNKSWVFFRELTGDGPIGALGAPVRGRVSLAAD
                     PLFTPLGTPVFLSLNPALAGATMANGLWVAEDTGGAIKGANRFDTFWGAGAEARQVAG
                     GLIGHGQAVMLLPLGTLARLGNR"
     gene            2803..3396
                     /locus_tag="VFF98_09280"
     CDS             2803..3396
                     /locus_tag="VFF98_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZU63864.1"
                     /translation="MARPRRRILSPEEQALWARVVETVEPLRQPAAAPPPAEAAQPTP
                     ASPPPRRIRGRVPAPLPAPAPPPPSRPPRDALGFERGGLDGGWDKRLAKGLVGPDFTL
                     DLHGATLETAYTRLDHGLALALAQGARMVLLITGRPRPAPGPMDRGSHRGVIRAKFQD
                     WLASGPHASRIAALRPAHPRHGGPGAVYIVLRRAKGD"
     gene            complement(3663..5249)
                     /gene="ahpF"
                     /locus_tag="VFF98_09285"
     CDS             complement(3663..5249)
                     /gene="ahpF"
                     /locus_tag="VFF98_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HZU63865.1"
                     /translation="MLEPALKQQLSQYLAMLREPIELVASLGEDAKSAQTRELLDEIA
                     SLSDKVSARFDGEDARRPSFTISPVAGAHGRVRFAGLPLGHEFTSLVLALLWAGGHPP
                     KVADETLAAIADLPGGSAFEMYFSLTCHNCPDVVQALTLIALRNPGYTATMIEGGTYQ
                     AEVEARGVMAVPAAFKDGEMWLSGKKTLEEIVAALDVGAEAKAAAAINAKAPFEVLVV
                     GGGPAGAAAAVYTARKGIRTGVAAERFGGQVLDTLGIENFISVPHTEGPKLAAALETH
                     VRDYEVDIINLARAEQLIPAAEEGGYHEVVLANGASLRARSIVLSTGARWRNMNVPGE
                     KDYANKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDTQLRA
                     DAVLQAKLRSLANVRILTNAQTTEVLGDGAKVNALAYKDRATGEAHKVELEGIFVQIG
                     LVPNTEWLKGTLALSPRGEIEVDARGATSLPGVFAAGDCTTVPYKQIVIAMGAGATAA
                     LSAFDYLIRLPATEQVAEPA"
     gene            complement(5344..5907)
                     /gene="ahpC"
                     /locus_tag="VFF98_09290"
     CDS             complement(5344..5907)
                     /gene="ahpC"
                     /locus_tag="VFF98_09290"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HZU63866.1"
                     /translation="MSLIGYSIQPFTATAYKDGAFVDVSDADIKGKWSVFFFYPADFT
                     FVCPTELEDLQNEYAGLQALGVEVYGVSTDTHFSHKAWADTSPAIGKIKYFLVGDQNH
                     DLTKQFNNLREGVGLADRGTFVTDPDGVVQLVEVTPEGVGRNAAELVRKIKAAKYWRE
                     HPGQVCPAKWEEGAETLAPSIDLVGKI"
     gene            complement(6127..7224)
                     /gene="aroC"
                     /locus_tag="VFF98_09295"
     CDS             complement(6127..7224)
                     /gene="aroC"
                     /locus_tag="VFF98_09295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZU63867.1"
                     /translation="MSWNSFGRIFRFTTWGESHGPALGAVVDGCPPGLAIDEGMIQPF
                     LDARRPGQSKFTTQRNEPDQVQILSGVFEAPDGVRRTTGTAISLMIENTDQRSKDYSE
                     VAKAYRPGHADYAYDAKYGFRDYRGGGRSSARETAARVAAGGVARLVIPEVTILAYVA
                     EIGGDAIDPAQFDAAQIRANPFFCPDAAAAERWAQKVDAARLAGSSLGAVVECVATGV
                     PAGWGAPLYAKLDADLAAAMMGINAVKGVEIGDGFAAARLTGEQNADPMRPGNDGPTF
                     TANHAGGIAGGISTGQPVVVRVAFKPTSSILTPMPTITRGGEATELFTKGRHDPCVGI
                     RGVPVVEAMMALVLADHKLVDRAQCAGRPSF"
     gene            7331..7690
                     /locus_tag="VFF98_09300"
     CDS             7331..7690
                     /locus_tag="VFF98_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HZU63868.1"
                     /translation="MTTTLYGIPNCDTVKKARAWLDARGIAYAFHDYKKAGADAGKLA
                     GWIAAAGLDKVLNRAGTTFKKLPPEAREGLDEAGAIALMVAAPSMIKRPIVEYGGVEH
                     AGGLLVGFRPEEWEAAF"
     gene            complement(7698..8171)
                     /gene="dut"
                     /locus_tag="VFF98_09305"
     CDS             complement(7698..8171)
                     /gene="dut"
                     /locus_tag="VFF98_09305"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZU63869.1"
                     /translation="MPSHDADVPVQVKRLPHGQGLPLPAYATHGAAGMDVVAAEDVVL
                     APAQRHAVATGLALAIPDGYEIQVRPRSGLALKHGIGAPNAPGTIDSDYRGELKIILI
                     NHGAEPFAIHRGDRIAQLVLAPVVRGQWREVDELDETLRGEGGFGSTGGVVNLQK"
     gene            complement(8158..9363)
                     /gene="coaBC"
                     /locus_tag="VFF98_09310"
     CDS             complement(8158..9363)
                     /gene="coaBC"
                     /locus_tag="VFF98_09310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZU63870.1"
                     /translation="MSGQRILLVVGGGIAAYKACELVRLIRKAGGAVTCVLTEGASHF
                     VTAMTLAALSENPVHTSLWDLTREAEMGHIQLSRAADLVVVCPATADLLAKMAAGLAD
                     DLATTLLLATDKPVLAAPAMNVRMWQHAATQANVATLRARGVAVMEPDEGAMACGEFG
                     PGRLPEVERIAEAIGTALAPQGRLLAGRHVLITAGPTHEPIDPVRYIANRSSGRQGFA
                     IAAAAAAAGARVTLVAGPVQLPTPAGVLRVDVETAREMAAAVEAALPADVAVMVAAVA
                     DWRVAGEAAQKLKKDGSGAVPPLALVENPDILAGLCASPRRPALVVGFAAETNDVIAH
                     AIAKRARKGADWIVANDVSAAGPDGGAMGGARNAVHIIGAAGVDSLPLMAKEAVATAL
                     VERIADALS"
     gene            complement(9422..10534)
                     /locus_tag="VFF98_09315"
     CDS             complement(9422..10534)
                     /locus_tag="VFF98_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63871.1"
                     /translation="MRAAAPRWDRFAALPRGWARAVLAAAVLLLLASAIVPITVGHGE
                     AASPPSVLKVDPKSAARARFDDLELYDHAIARIRAGENYYAFIVSEHRAAHYPVRPGL
                     AVRLPTLAYFDAWAGAAGQLAAALALLVGVALAWWARLGAEPGAARYRLWAMAFLLVN
                     ASLGLNRYFFVLHELWAGMLIALSLGLHRVAGEGVKARWLGAWLAAALALALRELALP
                     YVLLMAALAFWRGDRREGAAWVLLAGLFGVGLGWHLHLVAAHTSLADPVGPSWLALRG
                     LGGWLSDVVLSSNLRFLPHWLAGPLAVLMVLGWAGWKSPLGTTAALFAAGYALAFMIA
                     GRDDNYYWGAMVAPSLFAGLAFAPMALRGLWRAALA"
     gene            complement(10534..12090)
                     /gene="ubiB"
                     /locus_tag="VFF98_09320"
     CDS             complement(10534..12090)
                     /gene="ubiB"
                     /locus_tag="VFF98_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HZU63872.1"
                     /translation="MASHTTHTLRLLRWGRILARHGALRIIEEGQNTPAPVKRLCRIA
                     RFGTRQPAVPDYAGAFQAIGPAAIKLGQTLATRPDLVGEEAARNLLSLQDQLPPVPFA
                     AIRAEIEAAFGRPLEELYAEVETQPVGAASIAQVHRARLHDGRQVAVKVLRPGIREKF
                     ARDIATYEWAAAHLEALGGEATRLRPRLVIANFRRWTARELDLRREAASASELAEAMA
                     GAGGYRVPAIDWDRTCGRVMTLEWVGGFKLSDHAALDASGVDRADLARRLVLTFLRQA
                     IAAGFFHADMHQGNLFIESDGTIVAIDFGIMGRINRQARVWLAEILYGLTTGNYRRVA
                     EIHFEAQYVPSHHSVDEFATALRAVGEPMRGRPVKDLSVGQMLDGLFAITRDFDMATQ
                     PHLLLLQKTMVMVEGLATSLDPDINMWDIAGPYVADWIRDELGPEAAVADRLHGLAKT
                     LLRLPGLLARVEESVPAKGGAPEPPPLPDVPLVWEARGRVPQLLGYVAAALVGGALTW
                     AGLVLAGLAG"
     gene            complement(12095..12850)
                     /locus_tag="VFF98_09325"
     CDS             complement(12095..12850)
                     /locus_tag="VFF98_09325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU63873.1"
                     /translation="MTDSHTATDSDTVSFGYEDIAAGEKEARVGAVFSSVAKKYDIMN
                     DAMSAGLHRVWKDRFVRRVKPQRHEQILDMAGGTGDIAFRMAAHGASITVSDINQDML
                     DVGIERAMQRGIDGLVWSRQNAEELSFESRVYDAYTIAFGIRNVTHVDKALAEAYRVL
                     KFGGRFFCLEFSTTEWPGFREVYDAYSHRLVPQIGQMIAGDAESYRYLIESIRRFPPM
                     PRFESMIRTAGFVNTKVEPILGGLVAIHSGWKI"
     gene            12908..13735
                     /gene="mutM"
                     /locus_tag="VFF98_09330"
     CDS             12908..13735
                     /gene="mutM"
                     /locus_tag="VFF98_09330"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZU63874.1"
                     /translation="MPELPEVETTVRGLAPFLEGQVIARVETRRAGLRRPFPADLAQM
                     LTGARVIGLGRRAKYGLLHTDRALTMVFHLGMSGRWRIEPETIDKHDHLLLTTGAGVL
                     LALNDPRRFGSVDLVETAALASWPAFAALGPEPLGEELTPAHLAAAFAGRVAPVKGLL
                     LDQAIVAGLGNIYVCEALHRAGISPLREGGRITRKGLERLVPAIKAVLAESIRAGGST
                     IRDYAQPNGELGYFAANWRVYGREGEPCSAKEGACTGTIRRVVQGGRSSFWCPRCQR"
     gene            14052..14312
                     /gene="rpsT"
                     /locus_tag="VFF98_09335"
     CDS             14052..14312
                     /gene="rpsT"
                     /locus_tag="VFF98_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZU63875.1"
                     /translation="MANTPQARKRIRRNERRAEINGARLSRIRTFVKKVEAAITAHDK
                     EAASVALKAAQPELDRGVARGVLHKNTASRKLSRLSKAVSAL"
     gene            14858..16300
                     /gene="dnaA"
                     /locus_tag="VFF98_09340"
     CDS             14858..16300
                     /gene="dnaA"
                     /locus_tag="VFF98_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZU63876.1"
                     /translation="MEDQEAIDLAADWADISQGLRKDLGQQLFNQWVRPIQLGSFCKE
                     TGTLDLFLPTEFAANWVADRFADRLSLAWKIARAEVRHVRISVHPGRRSMPDLRLARD
                     NGAERPANDAPAQAGRSLGNAASDVVAHIATDTLGLASIGLDPSLTFASFVTGTSNVL
                     AFNAAQRMAATEKPQFSPLYLKAATGQGKTHLMHAIGHAFLANHPRARIFYCSAERFM
                     IEFVQALRQNQMIEFKSRLRGFDLLMIDDIQFIIGKASAQEELLYTIDALLAEGKRLV
                     FAADRAPQALDGVEQRLLSRLSMGLVADIQPADIELRRLILDNRLARVGSAMHVPGDV
                     VEFLARTINRNVRELVGGLNKLIAYAQLTGQPVSLALAEDQLTDILSANRKRITIDEI
                     QRTVCQFYRVDRTEMSSKRRARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTV
                     IHAVRLIEDLRTRDADIDGDVRSLLRQLES"
     gene            16579..17358
                     /locus_tag="VFF98_09345"
     CDS             16579..17358
                     /locus_tag="VFF98_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HZU63877.1"
                     /translation="MTLLPPDRLARFARHIVLPEVGGVGQVALAGAHAVLVGCGGIGA
                     PALQYLAAAGIGRLTLIDDDRVEASNLQRQTIFAEADIGAPKAEAAAGWVARFDPALQ
                     VKAHRLRLTGANAAALVAGADVVLDGCDNFATRLAVSDACVAAGVPLTSAAVGRFQGQ
                     VANFAGHLPDQACYRCFVGEAFDAADCDTCAADGVLGAFVGLVGTLAAMSALRAILHG
                     AGVPGPLADPQWGRLQLVDGLAPTLRAIAIPADPACRACAR"
     gene            complement(17355..18062)
                     /locus_tag="VFF98_09350"
     CDS             complement(17355..18062)
                     /locus_tag="VFF98_09350"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZU63878.1"
                     /translation="MGTIRNIVILTGAGCSAESGIDTFRDGGGLWEQHRVEDVATPEA
                     FARDPDLVLRFYDMRRAAIQTKAPNAAHHALARLEREWPGRVTLVTQNVDDLHDRAGS
                     RALIHMHGAHLSAWCTACDERVPWRGALIDRPACPACGVRQLRPDIVWFGEMPYRMDD
                     IHAAMRAADLFVSIGTSGAVYPAAGFVRMARELGAETLELNLERSQGSAWFHTSRFGP
                     AGELVPAWVAGMLAGAA"
     gene            18098..18826
                     /gene="dapB"
                     /locus_tag="VFF98_09355"
     CDS             18098..18826
                     /gene="dapB"
                     /locus_tag="VFF98_09355"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZU63879.1"
                     /translation="MARMGIIGSEGRMGKALAEAVAAAHHQLAGGIDRDGDANALALA
                     SDVLIDFSAPAALEANLDAAAAAGIAILVGTTGLEERHHWLIDRAAEQIPVLQTGNTS
                     LGVNMLAFLVEEAARRLGEDWDIEIVETHHRMKVDAPSGTALLLGEGAARGRQIALAD
                     HAVRGRDGITGKREAGTIGFASLRGGSVAGDHTVHFLADNERLSLSHLAENRGIFARG
                     AVKGADWLIGKAPGRYGMKEVLGL"
     gene            complement(18837..19970)
                     /locus_tag="VFF98_09360"
     CDS             complement(18837..19970)
                     /locus_tag="VFF98_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HZU63880.1"
                     /translation="MQNIQIDAAFDAGNIEVLAISGAEARLAIRKDRNSDFFQWFHFR
                     VSGAGGRVLVLRLTGLKAAAYPDGWAGYRACVSADGETWTRADTAWDPAADNGTLTIR
                     HQPAGDLVWFAYFAPYAMERHRALVSRTAALPGVGYRRLGHSLDGQPIDCLELGEGVQ
                     QVWLQARQHPGETMAEWWMEGALELLCDPADPHGRWLRQRCRFHIVPNANPDGSARGH
                     LRTNAAGVNLNREWHAPTPERSPEVLAIRTAMDESGVDFCMDVHGDEAIPAVFLAGFD
                     GIPALTDAQAAGYRRYVSLLDALTPDFQTARGYPSAPPGRANLTMATNQIAQRFGAVA
                     MTLEMPFKDHDPLPSAETGWSPRRSHLLGRACMVALAQWLAAG"
     gene            complement(20012..20794)
                     /locus_tag="VFF98_09365"
     CDS             complement(20012..20794)
                     /locus_tag="VFF98_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63881.1"
                     /translation="MSVFVLRPALLLAGSALAVGALVVATPVLAQPGWGGGWGRGGFD
                     GPGGWDGPATASGADPREGRVQAESFVAPDGVDLLGRGRIAVQAGPGGSADERQEATF
                     EAAIIDQLAKAGYDTLNPDPTGGQVTEVRLVRDVVVPEEPKKSPWSGAAEMGVSNHGS
                     MMAVQLNYDASKPRKALVNTHLELRIRERTTGQPLYEGRADIVTREGDSHWSDGAIAA
                     RLAAAIFAKLPHGGPATPPPPPAAAVPIPIDPAPQPAAKPAS"
     gene            21060..22061
                     /gene="galE"
                     /locus_tag="VFF98_09370"
     CDS             21060..22061
                     /gene="galE"
                     /locus_tag="VFF98_09370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZU63882.1"
                     /translation="MTKLPVLVTGGAGYIGSHAVLALKDAGWPVAVVDNLSTGFRFAV
                     PEGVPFYEGDIADAALLARIFAEQGTQAIMHFAGSIIVPESVENPLKYYRNNTANSRS
                     LIEAAVNGGVKHFLFSSTAATYGIPEVSPVTEDSPRQPINPYGMSKLMTEIMLADVAR
                     AHPLNFCALRYFNVAGADPEARTGQSTAGATHLIKVAVEAALGKRESVGVFGTDYATP
                     DGTGVRDYIHVSDLAAAHVLALEALIADPAASLTMNCGYGRGFSVLEVLDAVDRVTNR
                     TITRVLGPRRAGDPDSLISDNARIKATLPWVPKYADLDVIVAHALAWERRLTEIRGG"
     gene            22162..22287
                     /gene="ykgO"
                     /locus_tag="VFF98_09375"
     CDS             22162..22287
                     /gene="ykgO"
                     /locus_tag="VFF98_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HZU63883.1"
                     /translation="MKIRNSLKSLKDRHRDNRVIRRRGRTYVINKTQRRFKARQG"
     gene            22473..23135
                     /locus_tag="VFF98_09380"
     CDS             22473..23135
                     /locus_tag="VFF98_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZU63884.1"
                     /translation="MHSPSPAQTLGPATPVTAAPVTAVVFDIGHVLIDWDLRCLFAKL
                     IDDPARLDWFLAHVVTKEWHFEHDAGKPFAQMVAERSALFPEEAALIAAYADRWLETL
                     PGPIPGTPALVERLAARGVPLYAITNFGDEAFARFRPTFPVLAHMGDIVISGVEKLVK
                     PGEEIFHLAARRFGHAPAGMLFIDDNAANIATAARLGWQTHHFTGGARALEADLVARG
                     LI"
     gene            23135..23788
                     /locus_tag="VFF98_09385"
     CDS             23135..23788
                     /locus_tag="VFF98_09385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU63885.1"
                     /translation="MRALFAVALLAAALPIAANAAPLRHAPAAHAPEPEAQLTADERV
                     ELVTEAGRIVLSLDGVHAPATTRNFLRYVMQHRFDGIIFYRAMHLPWGDPPNGLIQAG
                     VRGDPKRLLPPIAHEPTDATGIHHKAGAISMARFAPGTATGDFSILLSDLDGLDADPK
                     ASDADARAGYAAFGHVVEGMDVARRIFDSPIDPAAGEGPLKGQMLARPVKILSARRL"
     gene            complement(23809..>24273)
                     /locus_tag="VFF98_09390"
     CDS             complement(23809..>24273)
                     /locus_tag="VFF98_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU63886.1"
                     /translation="GHLGDAGFACGRAPRCAVEYQARLSGPLVDRIDLHVDVAAVPAT
                     ALAAAAPAESSASVALRVAAARAAQTARLAGTPMRCNAELEGEALERFATPDAQGQAL
                     LQRAAEAMRLSARAYTRIRRVARSIADLAGAEGIGRAHVAEALSYRRLPPRG"
BASE COUNT         3867 a         8551 c         8084 g         3771 t
ORIGIN      
        1 cggccagcgc cggcagccgc gggctgcgcc atcaggcgtg gccggccggc tcgcccagcg
       61 catcggcgcc atcggcctgc tgggcctggg cggcgagctg ctgcaggtag atgccgttga
      121 agtcgatcgg ctccagcagc agcggctgga agccggcatc gcgcacggca tcggccacca
      181 ctcggcgggc aaacgggaac aggatgcgcg gcgcctcggc aaacatgaag gcgtgcatgt
      241 tggcctcatc aaggttgcgc atgccaacca ggcccgcata ggtgagatcg accagatagg
      301 cgacgccggc ctcggccttg gcggtcagct tgatcttcag ctccacctca tgcacctcgc
      361 cctgcagcgg gtgggcggcg atgttgaact gcacgtccat ctcgggcgtg tcggcccagg
      421 catagcaatc cggcgcgttc gggttctcga ccgagagatc cttcacatat tgcgagatga
      481 cccctgcggc cggcagcgtg tcggcatcga ggttgagatt gctggtaaca ttgccttcat
      541 cggccatggc tgtggtgctt tccgtgtctt gcgatgcgta actgcggtga ttgcgaatca
      601 atacccgcgc cgatggcgcc cgcgcgccgc caacatgggc ctgatgaaac agcgcgccta
      661 gcatccgggc gggcgggcgg caatgggcgc aggcgccgca tgggggcatg gggcgcaaca
      721 gccacagccg gcccgctggc gcatccgggc ggcagattgt ggcaaatttt tcccccggag
      781 ccggccgtcc atgtgttgtt catttgtgtt tggcacctat gtagggcaag gtacacctcc
      841 gccagaaagg cgggaaacac tggaaacctg cctgtgactg ttgaaatcgt catccttgcc
      901 atgatcgcgg cgctgctggg gctgaggctc tattcagttc tcggccgccg ggcggagcat
      961 ggcgaggagc cgctgcaaag ccggcaggca ggccagcagg cccagcccgc caccgcgcct
     1021 gcccgcctgc ccgaacgcgc cctgccgcag caaagcgccc cgcgcgcccg cgagatcccc
     1081 gcggtggtgc cggcggtgga aaaggccctg cgcgagatca tgggcgccga tcgccggttt
     1141 gatcccaacg cctttgttga tggcgcgcgc caggcctacc ggatgatcct cgacgccttc
     1201 tggcgcggcg acaaggacga gctggcgcat ctgtgcgatc cggtggtggc cggccgcttt
     1261 ggcgaggcga tcgatgcccg caaaaccgcc ggcgaagtgc ccgacaaccg cgtggtgcgc
     1321 atcaacgagg cggcgattgt cggcgcggcg ctggctggca ccaccgcccg cgtggcgatc
     1381 cgcttcgtca ccgacatcgc cgctgtcacc cgcgatggcg aggggcgggt gatcgccggc
     1441 tcgctcgacg atgcggtgga agtgcgcgac gtgtggacct tctgccgcga tatccgcgcc
     1501 accgatcccg actggctgct ggacgaaacc gacgagggct gatttcccgt gttcaagacc
     1561 attgcaggac cccgctggct ggtgctggcg gccatgctca gccttgccgg ctgcgggcgc
     1621 atcattcccg aaggccatcc gcccggcgcg ctgccgccgc ccggccctgc cgccccgggc
     1681 cagccctatg cccccgcgcc gctgccgccc aatgccgtgg cgctgggggt gcatcgcggg
     1741 cccgatcccg tcagcctggg cctgacggca gccaatgccg ccccggcgct ggcggccttc
     1801 cgcgaggcct gcggccacct caccggccgc gcagacccca gcggcctgac ccagccgatc
     1861 gactggcagg ccccctgcgg cgccgccaaa agctggcgcg ccgacgaggc cgaccgcttc
     1921 ttcaccacct tcttcgaaag cgtggtggtg ggcgagggca atgccaatgt caccggctat
     1981 tacgagccgg agatctcggg cgtgaaaacc cgccagcccg gcttcgacgt accgatctat
     2041 ggcctgccgg gcgatctcgt ccacgcgcgc cccggcgatg cgcaggtgaa gcccaacggg
     2101 caaaccccct ttggccgcta tgacgagctg ggccacttct cgccctatta cgagcgcgcc
     2161 gagatcgaga acggcatgct ggccaaccgc gggctggaaa tcggctgggc ggcagatccg
     2221 gtggagctgt tcttcctgca gatccagggc tctggccggc tgcgcgcgcc cgatggcagc
     2281 atcacccgca tcggctatgc cgccgacaat ggccgcgatt acaccagcat cggcatcatc
     2341 ctgcgcgatc gcgggctgat cggcagcggg cccgggcaat atcccggctc gatgcagggg
     2401 atcatgcaat atctgcgcga ccacccctat gagggccgcc agctgatgga catgaacaaa
     2461 agctgggtgt tcttccgcga gctgaccggc gatggcccga tcggcgcgct gggggcgccg
     2521 gtgcgcgggc gcgtgtcgct ggcggcagat ccgctgttca ccccgctggg cacgccggtg
     2581 ttcctctcgc tgaaccccgc gctggccggc gccaccatgg ccaatggcct gtgggtggcc
     2641 gaagataccg gcggcgccat caagggcgcc aaccgcttcg acactttctg gggcgcgggc
     2701 gccgaggcgc ggcaggtggc cggcgggctg atcggccatg gccaggcggt gatgctgctg
     2761 ccgctgggca cgctggcgcg cctgggcaac cgctgagcgg gcatggcccg ccctcgtcga
     2821 cggatcctca gcccggaaga gcaggcgctc tgggcccggg tggtggaaac ggtggagcca
     2881 ctgcgccagc cggccgccgc cccgccgccg gctgaagccg cccagcccac ccctgcctca
     2941 ccaccgccgc ggcgcatccg cggccgggtg ccggcgcctc tgcccgcccc cgcgccgccg
     3001 ccgccctctc gccccccgcg cgatgcgctg ggcttcgagc gtggcggcct cgatggcggc
     3061 tgggacaagc gcctggccaa ggggctggtg ggccccgatt tcacgctgga cctccacggc
     3121 gccacgctgg aaaccgccta cacaaggctg gaccacgggc tggcgctggc gctggcgcaa
     3181 ggcgcgcgga tggtgctgct gatcaccggc cgcccccgcc ccgcccccgg ccccatggac
     3241 agaggcagcc atcgcggcgt tatccgcgcc aagtttcagg actggctggc cagcgggccc
     3301 catgccagcc gcatcgccgc cctgcgcccg gcccatccgc gccatggggg gccaggggcg
     3361 gtgtatatcg tgctgcggcg ggcgaagggg gattagggtg cgagggttaa caccctcgcg
     3421 ctcccatgat ctgtcggcct cagagtgctc cttcggcgga gcggcaggcg gcgaggccgc
     3481 aatgatcctt gcctccggcg gtgcggcgtt gcgcttgtgc cgaacccttg gcaccccgcc
     3541 gacaggtaac gggagcgcga gggtattaac cctcgcatcc ccttcccttc accccaaaaa
     3601 ccaagagccg gaggcacgca aggcacctcc ggctcagggg ttcgctcgct ggcgagaagg
     3661 gttcaggccg gctctgccac ctgctccgtg gctggcaggc ggatcaggta gtcgaacgcg
     3721 ctgagggcgg cggtggcccc tgcccccatg gcgatgacga tctgcttgta gggcaccgtg
     3781 gtgcagtctc cggcggcgaa cacgcccggc aggctggtgg cgccgcgggc atccacctcg
     3841 atctcgccgc gcggcgaaag cgccagcgtg cccttcagcc attcggtgtt gggcaccagg
     3901 ccaatctgca cgaaaatgcc ttccagctcc accttgtggg cctcgccggt ggcgcggtcc
     3961 ttgtaggcaa gggcgttcac cttcgcgcca tcgcccagca cctcggtggt ctgcgcgttg
     4021 gtgaggatgc gcacattggc cagcgagcgc agcttggcct gcagcacggc atcggcgcgc
     4081 agctgcgtgt caaactcgat cagcgtgaca tgggcgacga tgccggccag atcgatcgcc
     4141 gcttccacgc ccgaattgcc gccgccgatc accgccacgc gcttgccctt gaacagcggg
     4201 ccatcgcaat gcgggcaata ggccacgccc ttgttggcat aatccttctc gcccggcacg
     4261 ttcatgttgc gccagcgcgc gccggtggac agcacgatgc tgcgcgccct gaggctggcg
     4321 ccattggcca gcaccacttc gtgatagccg ccctcctcgg ccgccgggat cagctgctcg
     4381 gcgcgggcaa ggttgatgat atccacctca taatcgcgca catgggtttc cagcgcggcg
     4441 gccagcttgg gcccttcggt gtgcggcacc gagatgaaat tctcgatccc cagcgtatcc
     4501 agcacctgcc cgccgaagcg ttcggcggca acgccggtgc ggatgccctt gcgcgcagtg
     4561 tagaccgccg ctgccgcgcc cgccgggccg ccgcccacca ccagcacctc gaacggcgcc
     4621 ttggcgttga tggcagctgc cgccttggcc tctgccccca catccagcgc ggcgacgatc
     4681 tcttccagcg tcttcttgcc ggaaagccac atctcgccat ccttgaaggc ggcgggcacg
     4741 gccatcacgc cgcgcgcttc cacctcggcc tgataggtgc cgccctcgat catggtggcg
     4801 gtgtagccgg ggttcctcag cgcgatcagc gtgagcgcct gcaccacatc cgggcaattg
     4861 tggcaggtga gcgagaaata catctcgaag gcgctgccgc cgggcagatc ggcaatcgcg
     4921 gccagcgttt catcggccac cttgggcgga tggccgccgg cccacagcag cgccagcacc
     4981 agcgaggtga attcatgccc cagcggcagg ccggcaaagc gcacgcggcc atgggcgccg
     5041 gcaacggggg aaatggtgaa ggaggggcgg cgcgcatcct cgccatcgaa ccgggccgac
     5101 accttgtcgg acagggaggc gatctcgtcg agcagttcgc gcgtctgcgc cgatttggca
     5161 tcctcgccca ggctggcgac gagctcgatc ggttcgcgca gcatggcgag atattgcgag
     5221 agctgttgct tcagagcggg ttcgagcatg ttgggggatc cttatagagg gcagcaaggc
     5281 ccccggggcg ggcttgcgga cacagatgcc cgccccggga gggttgatcc tgccgaagcg
     5341 ggatcagatc ttgccgacga ggtcgatcga gggagcgagg gtttcagcgc cctcttccca
     5401 cttggccggg cacacctggc cggggtgctc gcgccagtac ttggcggcct tgatcttgcg
     5461 caccagttca gccgcgttgc ggcccacgcc ctcgggcgtc acttccacca actgcaccac
     5521 gccatcgggg tcggtgacga aggtgccgcg gtcagccagg cccacgcctt cgcgcaggtt
     5581 gttgaactgc ttggtgaggt cgtggttctg gtcgcccacg aggaaatact tgatcttgcc
     5641 gatggcgggc gacgtgtcgg cccaggcctt gtggctgaag tgggtgtcgg tcgacacgcc
     5701 atagacttcc acgcccagcg cctgcaggcc ggcatattcg ttctgcaggt cttccagctc
     5761 ggtcgggcac acgaaagtga aatcggccgg atagaagaag aacaccgacc acttgccctt
     5821 gatgtcggcg tcgctcacgt cgacaaaggc gccatccttg taggcggtgg cggtgaaggg
     5881 ctggatcgaa tagccgataa gcgacatgga attgctcctt tggtggtttg cgtggttgaa
     5941 cgaaccggga gccgggatag ggccctgcgc cgcagatgcg aagtgcggat tcccgattaa
     6001 tcagattggc aacgccgatt agtccattct gatcggaaat atcgatcagg gcgcgcgccc
     6061 gcgcaaaagc cgcccgctcc cccaaggaag caggcgggcc gggtgcgacc gcggcgtgtc
     6121 agttcatcag aaagaagggc ggccggcgca ttgcgcccga tcaaccagct tgtgatcggc
     6181 cagcaccagc gccatcatcg cttccaccac cggcaccccg cgaatgccca cgcacggatc
     6241 gtggcggcct ttggtgaaca gttcggtcgc ctcgccgccg cgcgtgatgg tgggcatggg
     6301 ggtgaggatg gaggaagtgg gcttgaaggc cacgcgcacc accaccggct ggccggtgga
     6361 aatgccgccg gcaatgccgc cggcgtggtt ggcggtgaag gtggggccat cattgcccgg
     6421 gcgcatcggg tcggcgttct gctcgccggt cagccgggcg gcggcaaagc catcgccaat
     6481 ttccacgccc ttcacggcgt tgatgcccat catcgcggcg gccagatcgg catcgagctt
     6541 ggcataaagc ggcgcgcccc agccggccgg aacgccggtt gccacgcatt ccaccaccgc
     6601 gcccagcgac gagcccgcca gccttgccgc atccaccttc tgcgcccagc gctcggccgc
     6661 ggcggcatcg gggcagaaga aggggttggc gcggatctgc gcggcatcga attgggccgg
     6721 atcgatcgca tcgccgccaa tctcggcaac ataggccagg atcgtcactt ccgggatcac
     6781 cagccgggca acgccgccgg ccgccacccg cgctgccgtt tcacgcgcgc tggaccggcc
     6841 gccgccacga tagtcgcgga agccatattt ggcgtcatag gcataatcgg catggcccgg
     6901 gcgataggcc ttggccacct cggaataatc cttggagcgc tggtcggtgt tctcgatcat
     6961 caggctgatc gcggtgccgg tggtgcggcg cacgccatcg ggcgcctcga acacgccgga
     7021 gaggatctgc acctgatcgg gctcgttcct ctgggtggtg aacttgcttt gccccggccg
     7081 gcgggcatcg aggaagggct ggatcatgcc ctcatcaatg gcaaggccgg gagggcagcc
     7141 atcgaccacg gcgcccagcg ccggcccgtg gctttcgccc caggtggtga atcggaagat
     7201 gcggccaaaa ctgttccagc tcatcgcctg cctttggcgc ccccgcgcgt gaatgtccac
     7261 acctgcccca atccggagcc tttgcgcccg cccgcccacc ggctagacag agccgcaaag
     7321 gagccccgcg atgaccacga ccctctatgg cattcccaat tgcgacacgg tgaagaaggc
     7381 gcgcgcctgg ctcgatgcgc gcggcatcgc ttatgccttc cacgactaca agaaggccgg
     7441 cgccgatgcc ggcaagctgg ccggctggat cgccgcggca gggctcgaca aggtgctcaa
     7501 ccgcgccggt accaccttca agaagctgcc ccccgaagcc cgcgaggggc tggatgaggc
     7561 cggcgccatc gcgctgatgg tggcggcccc ctcgatgatc aagcggccga tcgttgaata
     7621 tgggggcgtt gaacatgccg gcggcctgct ggtgggcttc cggccggaag aatgggaggc
     7681 ggccttctga ccgccgccta cttctgcagg ttgaccaccc cgccggtgga gccaaagccg
     7741 ccctcgccgc gcagcgtctc gtccagctcg tccacctcgc gccactggcc gcgcaccaca
     7801 ggggccagca ccagctgggc gatgcggtcg ccccggtgga tggcgaaagg ctctgcgcca
     7861 tggttgatga ggatgatctt cagctcgccg cgataatccg aatcgatcgt gcccggcgca
     7921 ttgggcgcgc caatgccatg cttgagcgcc aggcccgagc gcggccgcac ctggatctcg
     7981 tagccatcgg ggatggccag cgccaggccc gtggccaccg catggcgctg ggcaggcgcc
     8041 agcaccacat cctctgccgc caccacatcc atgccggcag cgccatgggt ggcataggcc
     8101 ggcagcggca gaccctggcc atggggcagg cgcttcacct gcacaggcac atcggcatca
     8161 tgagagggca tcggcaatcc tttcgaccag agcggtagcc accgcctcct tggccatcag
     8221 cggcaggctg tccacccctg cggcgccgat gatgtggaca gcattgcgcg cgccgcccat
     8281 cgccccgcca tccggccccg ccgcggaaac atcattggcc acgatccaat ccgccccctt
     8341 gcgcgcgcgc ttggcgatgg catgggcgat cacatcgttg gtttcggcgg caaagcccac
     8401 caccagcgcc gggcggcggg ggctggcgca caggcccgcc agaatatccg ggttttccac
     8461 cagcgccagc ggcggcaccg cgccggaacc atccttcttc agcttctgcg cggcttcgcc
     8521 cgccacgcgc caatcggcca cggccgccac catcaccgcc acatcggcgg gcagcgcggc
     8581 ctccacggcg gcggccatct cgcgggcggt ttccacatcc acgcgcagaa cgccggcggg
     8641 cgtgggcagc tgcaccggcc ctgccaccag cgtaacgcgc gcgccagccg ccgccgccgc
     8701 cgcggcaatg gcaaagccct gcctgccgct ggaccggttg gcgatatagc gcaccggatc
     8761 gatcggctca tgcgtggggc cggcggtgat cagcacgtgg cggccggcca gaagacggcc
     8821 ttggggcgcc agcgccgtgc caatcgcctc ggcaatgcgt tccacctcgg gcaggcggcc
     8881 ggggccgaat tcgccgcagg ccatggcgcc ctcatccggc tccatcacgg caacgccgcg
     8941 cgcgcgcagg gtggcaacat tggcctgcgt tgccgcatgc tgccacatgc gcacattcat
     9001 ggcaggggcg gccagcaccg gcttgtctgt ggccagcagc agggtagtgg ccagatcatc
     9061 ggccaggccc gcggccatct ttgccagcag atcggccgtg gccgggcaca ccaccaccag
     9121 atccgccgcg cgcgacagct ggatatggcc catctcggcc tcgcgcgtca gatcccacag
     9181 gctggtgtga accgggtttt ccgacagcgc cgccagcgtc atcgcggtga cgaaatggct
     9241 ggcgccctcg gtcagcacgc aggttaccgc gccgcccgcc ttgcggatca ggcgaacgag
     9301 ttcgcacgcc ttgtaggcgg caatgccgcc gcccaccacc agaaggatgc gctggccgga
     9361 caggcgcccg ccggaaagaa gatctgccgt catgccgcgc tgtctgcccg ttgccggccg
     9421 atcaggcaag cgcggcgcgc cacaggccgc gcagggccat tggcgcaaag gccagcccgg
     9481 caaacaggga aggcgccacc atcgcccccc aatagtaatt gtcgtcgcgc ccggcgatca
     9541 tgaaggccag ggcatagcct gcggcaaaca gggcggcagt ggtgcccagc gggcttttcc
     9601 agccggccca gcccagcacc atcaacaccg ccagcggccc tgccagccaa tggggtaaga
     9661 acctgaggtt gctggagagc accacatccg acagccagcc ccccagcccg cgcagcgcca
     9721 gccaactggg gcccacagga tccgccagcg aggtgtgcgc ggccacaaga tgcagatgcc
     9781 agcccagccc cacgccaaac agccccgcca gcagcaccca ggccgccccc tcgcgccgat
     9841 ccccgcgcca gaaggccagc gccgccatca gcagcacata gggcagcgcc agctcgcgca
     9901 gcgccagcgc cagcgccgcc gccagccagg cccccagcca gcgcgccttg acgccctcgc
     9961 ccgccacccg gtgcaggccc agcgacaggg caatcaacat ccccgcccac aattcatgca
    10021 gcacgaagaa atagcggttc agccccagcg aggcattgac cagcaggaag gccatcgccc
    10081 acagccgata gcgcgccgcc cccggctctg cccccagccg cgcccaccag gccagcgcca
    10141 cgccgaccag cagcgccagg gccgccgcca gctgcccggc agcgcccgcc caggcatcga
    10201 aataggccag cgttggcagc cgcacggcca ggcccgggcg caccggataa tgcgccgcgc
    10261 gatgttcgct gacgatgaag gcgtagtaat tctcgcccgc gcggatgcgg gcgatggcgt
    10321 ggtcatacag ttccagatcg tcgaagcggg cgcgggcggc cgatttgggg tccaccttca
    10381 gcacggaagg ggggctggcc gcctcgccat ggcccacggt gatcggcacg atggcgctgg
    10441 ccagcagcag cagcacggcc gctgccagca ccgcccgggc ccagccgcgc ggcagggcgg
    10501 caaagcgatc ccaccggggg gctgctgccc gcatcaacca gccaggcccg ccagcaccag
    10561 ccccgcccag gtgagcgccc cgcccaccag cgcggccgcc acatagccca gcagctgggg
    10621 cacgcgcccg cgcgcttccc acaccagcgg cacatcgggc agcggcggcg gctctggcgc
    10681 gccgcctttg gccggcacgc tttcctccac ccgcgccagc aggccgggca ggcgcagcag
    10741 ggttttggcc aggccatgca gcctgtcggc caccgccgcc tccgggccca gctcatcgcg
    10801 gatccagtcg gccacatagg ggccggcgat gtcccacatg ttgatgtcgg gatcgaggct
    10861 ggtggcaagg ccttccacca tcaccatcgt cttttgcagc agcagcaggt ggggctgggt
    10921 cgccatgtcg aaatcgcggg tgatggcaaa caggccatcc agcatctgcc ccaccgacag
    10981 atccttcacc ggccggccgc gcatgggttc gcccaccgcg cgcagggccg tggcaaattc
    11041 gtccaccgaa tggtggctgg gcacatattg cgcctcgaaa tggatctcgg ccacgcggcg
    11101 gtaattgccg gtggtgaggc catagaggat ctcggccagc cacacccgcg cctgccggtt
    11161 gatgcgcccc atgatgccga aatcgatggc aacgatggtg ccgtcgctct cgatgaagag
    11221 gttgccctgg tgcatgtcgg catggaagaa gccggccgcg atcgcctggc gcaggaaggt
    11281 gagcaccagc cggcgggcca gatccgccct gtccaccccg gaggcatcga gcgcggcatg
    11341 atccgacagc ttgaagccgc ccacccattc cagcgtcatc acccggccgc aggtgcggtc
    11401 ccagtcgatc gccggcacgc ggtagccgcc ggcgccggcc atcgcctcgg ccagttcgct
    11461 ggcagaggcc gcctcgcgcc tcaaatccag ctcgcgcgcg gtccagcgcc tgaaattggc
    11521 gatgaccagg cgcgggcgca ggcgagtggc ctcgccgccc agcgcttcca gatgggccgc
    11581 ggcccattcg taggtggcga tgtcgcgggc gaatttctcg cggatgccgg ggcgcagcac
    11641 cttcactgcc acttgccggc catcatgcag ccgggcgcgg tgcacctggg cgatggaagc
    11701 cgcgcccacc ggctgcgtct ccacctcggc gtaaagctcc tccagcgggc ggccaaaggc
    11761 ggcctcgatc tcggcgcgga tggcggcaaa gggcaccggc ggcagctgat cctgcagcga
    11821 gaggaggttg cgcgcagctt cctcgcccac cagatcgggc cgtgtcgcca gggtttgccc
    11881 cagcttgatc gccgccgggc caatcgcctg gaaagcgccg gcataatcgg gcacggccgg
    11941 ctggcgcgtg ccaaagcggg caatgcggca caggcgcttc accggcgcgg gcgtgttctg
    12001 cccttcctcg atgatcctca gcgcgccgtg gcgggccaga atgcggcccc agcgcagcag
    12061 gcgcagcgta tgcgtggtat ggctggccaa ggggtcagat cttccagccg gaatggatgg
    12121 ccaccagccc gcccaggatc ggctccacct tcgtgttcac aaagccggcg gtgcggatca
    12181 tgctttcaaa ccgcggcatg ggcgggaagc ggcggatcga ttcgatgaga tagcgatagc
    12241 tttccgcatc gcccgcgatc atctgcccga tctgcggcac gaggcggtgc gaataggcat
    12301 catagacctc gcggaagccc ggccattcgg tggtggaaaa ttcgaggcag aagaagcgcc
    12361 cgccgaattt cagcacgcga taggcctcgg ccagcgcctt gtcgacatgg gtgacgttgc
    12421 ggatgccaaa ggcgatggtg taggcatcat acacccggct ttcaaagctc agctcctcgg
    12481 cgttctgccg gctccacacc aggccgtcga tgccgcgctg catcgcccgc tcgatgccca
    12541 catccagcat gtcctggttg atgtcggaga cggtgatgct ggcgccatgg gcggccatgc
    12601 ggaaggcgat gtcgccggtg ccgccggcca tgtcgaggat ctgctcgtgg cgctgcggct
    12661 tcacccggcg cacgaagcgg tccttccaca cccggtgcag gccggccgac atggcatcgt
    12721 tcatgatgtc gtatttcttg gccacgctgg agaagaccgc gcccacgcgc gcctccttct
    12781 cccctgccgc gatgtcttca tagccgaagg aaacggtgtc gctgtctgtg gcggtgtggc
    12841 tgtctgtcat ggcccgcgct ttaggaggtt ttgtgcggcc gcgcaaaggc tgataaggca
    12901 atggcccatg ccagaattgc cagaagttga aaccaccgtg cgcggccttg cccccttcct
    12961 ggaagggcag gtgatcgccc gggtggaaac ccgccgggcg ggcctgcgcc gccccttccc
    13021 cgccgatctg gcgcagatgc ttaccggcgc gcgcgttatc gggctgggcc ggcgggccaa
    13081 atatggcctc ctccacaccg accgggcgct gaccatggtg tttcacctgg gcatgtcggg
    13141 ccgctggcgg atcgagccgg aaacgatcga caagcacgac catctgctgc tgacaaccgg
    13201 cgccggcgtt ctgctggcgc tcaacgatcc gcgccgcttc ggctcggtcg atctggtgga
    13261 aaccgcagcg ctggccagct ggcccgcctt tgccgcgctg gggccggaac ccttgggcga
    13321 ggaactgacg cctgcccatc tggccgccgc ctttgccggg cgggtggcgc cggtgaaggg
    13381 gctgctgctc gatcaggcga tcgttgccgg ccttggcaac atctatgtgt gcgaggcgct
    13441 gcaccgcgcg ggcatcagcc ccttgcgcga aggcgggcgg ataacgcgca aggggctgga
    13501 acggctggtg ccggcgatca aggcggtgct ggccgaatcg attcgcgccg gcggctccac
    13561 catccgcgat tatgcccagc ccaatggcga gctgggctat ttcgccgcca actggcgcgt
    13621 ctatggccgc gagggagagc cctgcagcgc caaggagggc gcctgcaccg gcaccatccg
    13681 ccgcgtagtg cagggcggcc gatccagctt ctggtgcccg cgctgccagc gttaatggtt
    13741 gtggcgcgct catggttttg gcgcctttgc gggcgcagcg gcaccggccc acgcccccac
    13801 ccggaatttg gttttggcgc ccttgcaggc gcagcggcac cggcccacgc ccccacccgg
    13861 ccacccctag ggtactctcg ttgggtggcc gggtgggggc gtgggtcggt gccgcttccc
    13921 gaacacaagt tgttgacgat tcgccgcagg gccgctaagg gcgccgcttt ccgcagccgg
    13981 cttgccggtc gcagatccga aaaaccaccg caattgccgg cccttctcgc gggcccctac
    14041 cgaggacaga catggccaat acgccgcaag cccgcaagcg catccgccgc aatgaacgcc
    14101 gcgccgagat caatggcgcc cgcctttcgc gcatccgcac cttcgtgaag aaggtggaag
    14161 ccgccatcac cgcccacgac aaggaagccg cctctgtggc cctgaaggcc gcgcagccgg
    14221 agctggatcg tggcgtggcc cgcggcgtgc tgcacaagaa cactgccagc cgcaagctct
    14281 cgcgtctgag caaggccgtt tccgcgcttt gattcgcgga aaggcccggc agttttactg
    14341 ccatgccggc taagtaaatt tcacaggtcg gcattaatga tgagcttata catggggccg
    14401 gtgggcagac caccggcccc atgccgtttg cgcccctgcg cgaagcccgc gaaaatccat
    14461 gacgattcgg tgatgcttat tttttcttgc attttttcaa atagttattt ctatttatga
    14521 gttttccgcg cggttgcgct gtcaaggcaa ttatttcaat ttttttgcat gccagcgcct
    14581 tgccctttgc cgcgaacgct tccagaaaaa accccaccgg caaataaatc ggtcgggatt
    14641 cacacataga tcacgagggc gggccatcgc ttggccagcc gggtgggccc acaggttcac
    14701 acggcagggc aggcgcgact ctgccgcgat gtgtcttgcg cgttcgcata tcggtttcag
    14761 gaattgtcag gagcatatcg gggtgacggg aagcgtttgg ggtggacgac gaggggagtc
    14821 gactgcggcc aaaagccggg ctaccgggct gcgcgctgtg gaagaccagg aagccatcga
    14881 tctggccgcc gattgggcgg atatcagcca gggcctgcgc aaggatctgg gtcagcagct
    14941 gttcaaccag tgggtgcgcc ccattcagct gggcagcttc tgcaaggaga ccggcacgct
    15001 cgatctgttc ttgccgaccg aattcgccgc caactgggtg gccgaccgct ttgccgaccg
    15061 gctgagcctg gcttggaaga tcgcccgcgc cgaagtgcgc catgtgcgca tctcggtgca
    15121 ccccggccgc cgctctatgc ccgacctgcg cctggcccgc gacaatggcg ccgaacgccc
    15181 cgccaatgac gcccccgccc aggccggccg ctcgctgggc aatgccgcga gcgacgtcgt
    15241 cgcccatatc gccaccgaca cgctgggcct ggcctcgatc ggcctcgatc cctcgctcac
    15301 cttcgccagc tttgtcaccg gcacctccaa cgtgctggcc ttcaacgccg cccagcgcat
    15361 ggccgcaacg gaaaagcccc agttctcgcc gctgtatctg aaggcggcca ccggccaggg
    15421 caaaacccat ctgatgcatg ccattggcca tgcgtttctg gccaatcacc cgcgcgcgcg
    15481 catcttctat tgctcggccg agcggttcat gatcgaattc gtgcaggcgc tgcggcagaa
    15541 ccagatgatc gagttcaagt cgcgcctgcg cggcttcgat ctgctgatga tcgacgatat
    15601 ccagttcatc atcggcaagg cttccgccca ggaagagctg ctctacacga tcgatgcgct
    15661 gctggccgag ggcaagcgcc tggttttcgc cgccgaccgc gccccccagg cgctggacgg
    15721 ggtggagcag cggctgcttt cacgcctgtc gatgggcctg gtggccgata tccagccggc
    15781 cgacatcgag ctgcgccgcc tgatcctcga caaccgcctc gcccgcgttg gctctgccat
    15841 gcatgtgccg ggcgatgtgg tggaattcct cgcccgcacg atcaaccgca atgtgcgcga
    15901 gctggtcggc ggcctcaaca agctgatcgc ctatgcccag ctcaccggtc agccggtcag
    15961 cctggcgctg gccgaggacc agctgaccga catcctcagc gccaaccgca agcgcatcac
    16021 catcgacgag atccagcgca ccgtctgcca gttctaccgg gtggaccgca cggagatgtc
    16081 ctccaagcgg cgcgcgcgcg cggtggtgcg gccgcggcag gtggcgatgt atctctccaa
    16141 ggtgctcacg ccgcgttcct acccggaaat cgggcggaaa ttcggcgggc gcgatcactc
    16201 caccgtaatc cacgcggtgc gcctgatcga ggacctgcgc acgcgtgacg ccgatatcga
    16261 cggcgacgtg cgcagcctgc tgcgccagct ggaaagctga tctcacaagc tgtgcatggg
    16321 ctgtgaaaag ccctgtgcac tggccggggg caaccctgtg ggcaggctgt gggtgccttt
    16381 gcgcaaaagc tgtggaaggc tggcccaaaa gcctgtgcgc aggctgttga tggcctgtgg
    16441 ataaccctgt gcgcccgatg tgggcatgca gggcacaggc ctgtgcagcc gcggtgggca
    16501 aagggtgcac aggcggagga caacccctcg cccaacccgg ctttgcctaa cccggctttc
    16561 ccttctaaac cgcccggcat gactctcctg ccgccagacc gcctcgcccg ttttgcccgc
    16621 catatcgtgc tgcccgaagt gggcggcgtg gggcaggtgg cgctggcggg cgcccatgcg
    16681 gtgctggtgg gctgcggcgg cattggcgcg ccggcgctgc aatatctggc cgccgccggc
    16741 attggccgcc tcaccctgat cgacgacgac cgggtggagg ccagcaacct gcaacgccag
    16801 acgatctttg ccgaggccga tatcggcgca cccaaggccg aagccgccgc cggctgggtg
    16861 gcccggttcg acccggcgct gcaggtgaaa gcccatcgcc tgcggctcac cggcgccaat
    16921 gccgcagcac tggtggcggg cgccgatgtg gtgctggatg gctgcgacaa tttcgccacg
    16981 cgcctcgccg tctccgatgc ctgcgtggca gccggcgtgc cgctcaccag cgcggcggtg
    17041 gggcggtttc aggggcaggt ggccaatttt gccggccatc tgcccgatca ggcctgctat
    17101 cgctgctttg tgggcgaggc tttcgatgcc gccgattgcg acacttgcgc ggccgatggc
    17161 gtgctgggcg ccttcgttgg gctggtgggc acgctggcgg cgatgagtgc cctgcgcgcg
    17221 atcctccatg gcgccggcgt gcccggcccg ctggccgatc cgcaatgggg ccggctgcag
    17281 ctggtcgacg ggctggcccc caccttgcgc gccatcgcca tcccggcaga cccggcctgc
    17341 cgggcctgcg cgcgctaggc cgcccctgcc agcatcccgg ccacccaggc cggcaccagc
    17401 tcgcccgccg ggccaaagcg gctggtgtgg aaccaggccg aaccctggct gcgttcgagg
    17461 ttcagctcca gcgtctcggc gcccagttcg cgcgccatgc gcacaaagcc cgccgccgga
    17521 tagaccgccc ccgacgtgcc gatcgagacg aacagatcag ccgcgcgcat ggcggcgtgg
    17581 atgtcgtcca tccgataggg catctcgcca aaccacacga tatcgggccg caattgccgc
    17641 acgccgcagg ccggacaggc gggccggtcg atcagggccc cgcgccaggg caccctttcg
    17701 tcgcaggccg tgcaccaggc cgacagatgc gcgccatgca tgtggataag cgcgcgcgat
    17761 ccggcgcgat catgcagatc atcgacattc tgcgtcacca gcgtcacccg gccgggccat
    17821 tcgcgctcca gccgggccaa cgcatggtgg gcggcattgg gcgccttggt ctggatggcg
    17881 gcgcggcgca tgtcgtaaaa gcgcagcacc agatcgggat cacgcgcgaa ggcctcgggc
    17941 gtcgccacat cctccacccg gtgctgctcc cacaggccgc cgccatcgcg gaaggtgtct
    18001 atgccgctct cggcgctgca gccggcgccg gtgaggataa cgatgttgcg gatggtgccc
    18061 atggcgctaa ggaaagcgcc aaagcaggag aatttcaatg gcacgcatgg gcattatcgg
    18121 cagcgagggg cgcatgggca aggcgctggc cgaggcggtg gcggccgccc accaccagct
    18181 ggccggcggg atcgaccgcg atggcgatgc caatgccttg gcgctggcca gcgatgtgct
    18241 gatcgatttt tcggcaccgg ccgcgctgga agccaatctt gatgcggcag cggcggcggg
    18301 cattgccatc ctcgtgggca ccaccgggct ggaagagcgc caccactggc tgatcgaccg
    18361 cgccgccgag cagatccccg tgctgcagac gggcaatacc tcgctgggcg tcaacatgct
    18421 ggcctttctg gtggaagagg ccgcccggcg gctgggcgag gactgggata tcgagatcgt
    18481 cgagacgcat caccggatga aggtggatgc accttcgggc acggcgctgc tgctgggcga
    18541 gggcgccgcg cgcggccgcc agatcgcgct ggccgaccat gccgtgcgcg ggcgcgatgg
    18601 catcaccggc aagcgcgagg ccggcacgat cggctttgcc tccttgcgcg gcggcagcgt
    18661 ggcgggcgat cacacggtgc atttcctggc ggataacgag cgcctctcgc tcagccatct
    18721 ggcggaaaac cgcggcattt tcgcgcgcgg cgcggtgaag ggcgccgatt ggctgatcgg
    18781 caaggcgccc ggccgctatg gcatgaagga agtgctgggc ctctgaaagc cggcgcttag
    18841 ccagccgcca gccactgggc cagcgccacc atgcaggcgc ggcccagcag gtgcgatcgg
    18901 cgcgggctcc agccggtttc ggccgagggc agcggatcgt ggtccttgaa gggcatttcc
    18961 agcgtcatcg ccacggcgcc aaagcgctgg gcgatctggt tcgtcgccat ggtaaggttg
    19021 gcccggccgg gaggggccga aggatagccg cgcgcggtct gaaaatcggg cgtcagcgca
    19081 tcgagcaggg agacatagcg gcgataccct gccgcctgcg cgtccgtcag cgcggggatg
    19141 ccatcgaaac cggcgaggaa aacggccggg atcgcctcgt cgccatgcac atccatgcag
    19201 aaatccacgc cgctctcatc catcgccgtg cggatcgcca gcacttccgg gctgcgctcg
    19261 ggcgtcgggg cgtgccattc gcggttgagg ttcacgcccg ccgcattggt gcgcagatgg
    19321 ccgcgcgcgc tgccatcggg gttggcattg ggcacgatgt ggaagcggca gcgctggcgc
    19381 agccagcggc catgcggatc agccggatcg cacagcagtt ccagcgcgcc ttccatccac
    19441 cattcggcca tcgtctcgcc ggggtgctgg cgggcctgca gccacacctg ctgcaccccc
    19501 tcgcccagct ccaggcaatc gatcggctgg ccatcaaggc tgtggcccag ccgccgatag
    19561 cccacccccg gcaacgccgc cgtgcggctg accagcgccc ggtgccgctc catcgcatag
    19621 ggcgcgaaat aggcaaacca caccagatcg ccggccggct ggtggcggat cgtcagcgtg
    19681 ccattgtcgg cggccgggtc ccaggcggtg tcggcgcggg tccatgtctc gccatcggcc
    19741 gagacgcagg cgcgatagcc cgcccagcca tcgggatagg ccgccgcctt cagcccggta
    19801 agccgcagca ccagcacccg cccgcccgcg ccgctgacgc ggaaatggaa ccactggaag
    19861 aaatcgctgt tgcggtcctt gcggatggca aggcgggcct cggcgccgct gatcgccagc
    19921 acttcgatat tgccggcatc gaaggcggcg tcgatctgga tgttctgcat gaaaaggggg
    19981 atagccttgg gcgctgccag cggcaagcgg ctcaggaagc gggtttggcg gccggctggg
    20041 gcgccgggtc gatggggatg ggcacggcgg cggcgggcgg cgggggcggc gttgccggcc
    20101 cgccatgggg cagtttggcg aagatggcgg cggccaggcg cgcggcgatg gcgccatcgc
    20161 tccagtgcga atcgccctca cgcgtgacga tgtcggcccg gccctcatac agcggctggc
    20221 cggtggtgcg ctcgcggatg cgcagttcca gatgggtgtt caccagcgcc tttctcggct
    20281 tgctggcatc ataattcagc tgcaccgcca tcatcgagcc atgattggaa acgcccatct
    20341 cggccgcgcc cgaccacggg ctcttcttcg gctcttcggg caccaccaca tcgcgcacca
    20401 gccgcacttc ggtcacctgg ccgccggtgg ggtcggggtt cagcgtgtcg tagccggcct
    20461 tggccagctg gtcgatgatg gccgcctcga aggtggcttc ctggcgctcg tcggcgctgc
    20521 cgccggggcc ggcctgcacg gcgatgcggc cccggcccaa cagatcgacg ccatcgggcg
    20581 ccacgaagct ttccgcctgc acgcggccct cgcgcgggtc ggcgccagag gcggtggcgg
    20641 ggccatccca gccgcccggc ccatcaaacc cgccccggcc ccagccgccg ccccagcccg
    20701 gctgggccag cacgggcgtg gccaccacca gcgcgccaac agcaagagca gaaccggcca
    20761 gaagcaatgc cggacgcagg acaaagacgg acatggaata tcctcgaaga aaaacggtcg
    20821 ccgcacccat cccgatcccc atgatcgccg taagccgggc gggctttccg gaggatgaac
    20881 gcgcctgtgc ccctaatcct ttcgcaatgg caacatacgg ttgcgcaagc agcgcgatcc
    20941 ggggcattga tgcggcccgg cgcccacggc gcgttaaagt ttaacgcctt cagcgcatca
    21001 tccggttggc gccttcagcg cgttacaatt cagcgccttc agcgcatggc aggagactta
    21061 tgacaaaact tcccgtgctc gtgaccggcg gcgccggcta tatcggcagc cacgccgtgc
    21121 tggcgctgaa ggatgccggc tggccggtgg ccgtggtgga caacctctcc accggctttc
    21181 gcttcgccgt gcccgaaggc gtgcccttct acgagggcga tatcgccgat gccgcgctgc
    21241 tggcgcgcat ctttgccgaa cagggcacgc aggcgatcat gcatttcgcc ggcagcatca
    21301 tcgtgcccga atcggtggag aacccactca aatattatcg caacaacacc gccaacagcc
    21361 gcagcctgat cgaggcggca gtgaacggcg gggtgaagca cttcctcttc tcctctaccg
    21421 cggccaccta tggcattccc gaagtctcgc cggtgacgga agacagcccc cgccagccga
    21481 tcaaccccta tggcatgtcg aagctgatga ccgagatcat gctggccgat gtggcgcggg
    21541 cccatccgct caatttctgc gcgctgcgct atttcaacgt ggccggcgcc gaccctgagg
    21601 cgcgcaccgg gcaaagcacc gcaggcgcca cccatctcat caaggtggcg gtggaagcgg
    21661 cgctgggcaa gcgcgagagc gtgggcgtgt tcggcaccga ttatgccacc cccgatggca
    21721 ccggcgtgcg cgactatatc catgtgagcg accttgccgc cgcccatgtg ctggcgctgg
    21781 aagcgctgat cgccgatccg gccgcctcgc tcaccatgaa ctgcggctat ggccggggct
    21841 tctcggtgct cgaagtgctg gacgcggtgg accgggtgac caaccgcacc atcacccgcg
    21901 tgctgggccc gcgccgggcg ggcgaccccg attcgctgat ctcggacaat gcccgcatca
    21961 aggccaccct gccctgggtg cccaaatatg ccgatctcga tgtcatcgtc gcccatgcgc
    22021 tggcctggga gcgccgtttg acggaaatcc gcgggggctg atgcgcgggg gtgccccctc
    22081 gcccgcttga ctttttgccg ccaccccctt aacggcgcgg cttcgatttt tggggcggtc
    22141 ggccgcccgc cgaggaaaac catgaagatt cgcaacagcc tgaagtcgct caaggaccgc
    22201 caccgggaca atcgcgtgat ccgtcgtcgc ggccgcacct atgtgatcaa caagacccag
    22261 cgtcgcttca aggcccgcca gggctgaagc cacaccggca gcaaggatgg cgcctggccc
    22321 cggccgacgc cgcctacggc ccgtttcctg cccccccttg gcgccttgcg ccgggggcgg
    22381 caggaaacgg gccgtttgct gtgcgcgcct gccttgccgc agggccgcga tagaggctgg
    22441 ccctgcctgc cggccggcgc tatgcccgcg ccatgcacag cccctccccc gcgcaaacgc
    22501 ttggccctgc cacccctgtc accgccgccc ctgtcaccgc tgtcgtcttc gatatcggcc
    22561 atgtgctgat cgactgggat ctgcgctgcc tgttcgccaa gctgatcgac gatccggccc
    22621 ggctcgactg gttcctggcc catgtggtga ccaaggagtg gcatttcgag catgacgccg
    22681 gcaagccctt cgcccagatg gtggccgaac gcagcgccct gttccccgaa gaggccgcgc
    22741 tgatcgcggc ctatgccgac cgctggctgg aaacgctgcc cggccccatc cccggcaccc
    22801 ctgcgctggt ggagcgcctg gcggcccgcg gcgtgccgct ctatgccatc accaatttcg
    22861 gcgacgaggc ctttgcccgc ttccgcccca ccttcccggt gctggcacat atgggcgata
    22921 tcgtcatctc gggcgtggaa aagctggtga agccgggcga ggagatcttc cacctggccg
    22981 cccgccgctt tggccatgcg ccggccggca tgctgttcat cgacgataac gccgccaata
    23041 tcgccaccgc cgccaggctg ggctggcaaa cgcaccactt caccggcggc gcccgcgcgc
    23101 tggaagccga tcttgtggcg cgcgggctga tctgatgcgc gcgctgttcg ccgtggcgct
    23161 gctggccgcc gccctgccca tcgccgccaa tgctgccccc ctgcgccatg cgcctgccgc
    23221 ccatgcgccg gagcccgaag cccagctgac cgccgacgag cgggtggagc tggtcaccga
    23281 ggcggggcgc atcgtgctct cgctcgatgg cgtgcatgcg cctgccacca cgcgcaattt
    23341 cctgcgctat gtgatgcagc accgcttcga tggcatcatc ttctaccgcg ccatgcacct
    23401 gccctggggc gatccgccca atgggctgat ccaggccggc gtgcgcggcg atcccaaacg
    23461 gctgctgccg cccatcgccc atgaacccac cgatgccacc ggcatccacc acaaggccgg
    23521 cgccatttcc atggcccgct ttgcgcccgg cacggccact ggcgatttct cgatcctgct
    23581 gtcagacctc gacgggctgg atgccgatcc caaggccagc gatgccgatg cgcgcgccgg
    23641 ctatgccgcc tttggccatg tggtggaggg gatggatgtg gcccgccgca tcttcgattc
    23701 gcccatcgat ccggccgccg gcgaaggccc gctgaaaggc cagatgctgg cccggccggt
    23761 gaaaatcctc tctgcccgca ggctttgatt agcggcgccg gcccactctc acccccgcgg
    23821 cggcaggcgc cgataggaca gcgcttctgc cacatgcgcc cggccgatgc cctcagcccc
    23881 ggccagatcg gcaatgctgc gcgccacccg gcggatgcgc gtgtaggccc gggcggaaag
    23941 gcgcatcgcc tcggcggcgc gctgcagcag cgcctggcct tgcgcatcgg gcgtggcaaa
    24001 gcgctccagc gcctcgccct ccagctcggc gttgcagcgc atgggcgtgc cggccaggcg
    24061 cgcggtttgc gccgcgcgcg ccgcagccac ccgcagcgcc acgctggccg agctttccgc
    24121 cggggccgca gccgccagcg ccgtggcggg caccgcggcc acatccacat gcaggtcgat
    24181 cctgtccacc agcgggcccg acagccgcgc ctgatactcc accgcgcagc gcggcgcccg
    24241 cccgcaggca aagccggcat cgcccagatg gcc
//
LOCUS       DASRYE010000180        16977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_134244,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000180 DASRYE010000000
VERSION     DASRYE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16977
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134244"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1370)
                     /locus_tag="VFF98_09395"
     CDS             complement(219..1370)
                     /locus_tag="VFF98_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZU63887.1"
                     /translation="MSGHRGFARGGSAIVGAATFGIGACPGLAPVDMAARAALMALAD
                     AGMALGEVDALYTSAPHEALGGLELAEYLGLAPRVTDCNRTGGSAFEVYVQQAALALE
                     AGLIDCALIAYGANPASNPPPAVGMTRQSPWEAPYRPLAPISSYALAASRHMHLYGTT
                     RAQLAEVAVAARQWARLNPEAALRDPLTIEDVLAARMVSDPLGVRDCCLFNDGAAAIV
                     MVRAERARDCARRPVAVLGAAHAVTHREISSMPDLTVTGAAISGPQAFAQAGVGPGDI
                     DVAMLYDAFTITPILFLEDLGFCPKGEGGRFVAGGHVAPGGGLALNTNGGGLSCVHPG
                     MYGLFLLVEAVRQLRGEAGARQVPDAELAIAHGNGGVLSSAATVILARG"
     gene            complement(1367..1744)
                     /locus_tag="VFF98_09400"
     CDS             complement(1367..1744)
                     /locus_tag="VFF98_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZU63888.1"
                     /translation="MSQPPPLPHPEAEWRAHLAAGRFMLQRSRASGAFVFYPRIAQPG
                     TGAQDLEWVPACGEGRIHAATLVRRRNPAESYNVVLVDLAEGPRLMSRVDGIAADAVT
                     IGMPVKARIIEEEGQPLLVFVPA"
     gene            complement(1750..2520)
                     /locus_tag="VFF98_09405"
     CDS             complement(1750..2520)
                     /locus_tag="VFF98_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU63889.1"
                     /translation="MTITLARQGPVAIVTLDRPETKNAITHDMRGQLYGAFEDIALDH
                     EVRAVILTGAGGDFCSGMDVGAMGRGGITGSIDRMHTLNRIARAIHGLKKPTIAAVPG
                     VCVGVGFSYALACDIVLAGEKAKFAAIFRNIALAPDGGLVWHLRQLMGVQRARELVFS
                     GRIVRAEEAVALGIALEKVADATLMDRALELATSFATGPTVAMGLAKRQFDLSWNAGF
                     EQYLDMEATMQPIASRTEDHEEGLRAFREKRPPAFRGE"
     gene            2702..3883
                     /locus_tag="VFF98_09410"
     CDS             2702..3883
                     /locus_tag="VFF98_09410"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZU63890.1"
                     /translation="MKEAAIVATARTPIGKAHRGSLNNIHGASMAGHVIAAAVRRAGL
                     DPAEIEDVLLGSARCEGAVGSNIARQAALRAGLPISTCGVTLDRKCASGLQAIAFAAQ
                     RIRAGEGGIYVAGGVETVSLVDPHRNTFRNKDEWLLAHQPEIYMAMNRTGDIVAQRYG
                     ISRADQDAFALASQSRTAAAQQAGAFDEEIVPIDVIRNVVDRALAVQGQEAVTLARDE
                     CNRPGTELAALAQLKPLFEGGTVTAGNCSQLSDGASVCVVMDADEAARRGLPILGLYR
                     GMQVAGCAPDEMGIGPVLAVPRLLERLGLAAGDIDLWELNEAYASQALYCQRRLGLDP
                     ARVNVDGGAIALGHPFGMTGSRMAGHALLAGRRRGARHAVVTMCIGGGMGAAALFEIV
                     S"
     gene            4022..5005
                     /locus_tag="VFF98_09415"
     CDS             4022..5005
                     /locus_tag="VFF98_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZU63891.1"
                     /translation="MSVGNLDPTAAEQLDMYRRMALIKANDERSRKVIMSGRLVMPYY
                     SPRGQDVIPSAVSVNLTDDDYICTIYRGTHDMLAKGFPLKDMWAEVAGRTTGTCKGKG
                     GPMHLTCPEKGVMVTTGIVGSSMPIANGIAWGSQLKGDGRVTVAYFGDAAANIGAFHE
                     SLNLAAVWKLPVVFVCQNNEWGEHTAYEKTSNVRVADRAAAYGMPGERVDGNDPFAVY
                     AAARRAVARARAGDGPSLIEAMTYRFHGHVFGDQDAYMDKARKADAMANDPVLRFRAR
                     LVESGIAGEDELAAIEAAIEAAIDEAVDYALASPFPDVAELKRDVFAEELA"
     gene            5005..6039
                     /locus_tag="VFF98_09420"
     CDS             5005..6039
                     /locus_tag="VFF98_09420"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZU63892.1"
                     /translation="MAVSEKTKPAAEGAAARPAKVNILQAINAAMADAMAADDSVLVL
                     GEDVADPEEGGVCGVTRGLSSRFGDHRVRSTPISEQAIIGAAIGASLVGFRPVAEIML
                     MNFTTVAMDMIVNHAAKLRFMSGGQTHVPIVIRTMTGTGFASGGQHCDYLEAWFAHTA
                     GLKVVAPSSPRDAYGLMRSAIEDPDPVLFIENLPTYWLADEAPESGYRVPIGKANVLA
                     EGSDVTIIAYARMIQEAAPAIARLAEAGISAELIDLRSIAPWDRAAVLASVAKTGRAM
                     IVHEAVTPFGVGAEIAATLNEELFGRLKAPVKRLGGAFCPVPFSKPLETAFAPQTEDI
                     FAAACALVSA"
     gene            6064..6294
                     /locus_tag="VFF98_09425"
     CDS             6064..6294
                     /locus_tag="VFF98_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HZU63893.1"
                     /translation="MATEVLLPKLGFSMNEGTLAEWLVADGGQAVEGEPLYALESEKS
                     TQEVESPASGTLKIIAQIGETYEVGTVLAVIG"
     gene            6300..6608
                     /locus_tag="VFF98_09430"
     CDS             6300..6608
                     /locus_tag="VFF98_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63894.1"
                     /translation="MARYTLEACSRALAGREAEYAHWYRDVHLAEVVALPGFLSGRLH
                     QRLDAQGAQAGELVAHYAVETDAPAALLEALFAAAPAMRLTDSIDMASLRFSFLAEAA
                     "
     gene            6608..7351
                     /locus_tag="VFF98_09435"
     CDS             6608..7351
                     /locus_tag="VFF98_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZU63895.1"
                     /translation="MAGLLDGLAPWPAVDFSAFGPVETQPLSKIQAYVAAHLGRNWVA
                     IPHVTHQDEADVTALEEQRRSLAAAGGPKLTPVPLLVKALARALAAFPRFNASLDPAS
                     GQLVLKRYVHVGVAVDTPGGLLVPVIRDCDAKSAAAIATELAALSEKARTKGLAMAEM
                     AGGCITLSSLGHIGGTGFTPIINAPEVAILGVARARAVAQPAADGSLAWRQMLPLSLS
                     YDHRVINGADAARFCRFIADQLADPALFD"
     gene            complement(7382..8281)
                     /locus_tag="VFF98_09440"
     CDS             complement(7382..8281)
                     /locus_tag="VFF98_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63896.1"
                     /translation="MGGFLDGRAGLAGKVAAVIGGGGGIGAGVALALAEAGVDLALCD
                     IDAGALAATAARAQGLGRQVLACHADATCEAALDGFYADLAGAFPRLDILVNLAGGTR
                     RGPLADASRADIARDIHRNYGYVVQSVQAALPLLRRSGDGGSIVNFTTIEAHRGAAGF
                     AVYAGAKAATANFTRAIAVELGKEKIRANCIAPDTTPSAGNLAAMPADFAARFAALPQ
                     AARGKGLEMYIPRGEAPPIEALADAVLFLASDLSRFVSGTTLHVDGGTMAAAGFLDWP
                     FGDGFVPTPLAGTLGRLYRPGDA"
     gene            8398..9144
                     /locus_tag="VFF98_09445"
     CDS             8398..9144
                     /locus_tag="VFF98_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU63897.1"
                     /translation="MARFILVHGAWHGGWCWEEIVPRLEAAGHEALAPDLPGMGADAD
                     SGHVASLEEWAMFIAGAAAAGGGAGRQPAILVGHSRGGMVISRAAQLAPVGSVAHLAY
                     LAAAMPRPGQTMSELFDAAALSRMVDMTGAIARSADGRQLIFHDAATALRAFYGLCPA
                     GKGEAAFARLTPEPAGIRTARVELDAARYGQVARSYIRCERDMAVVPELQNRMIAAQP
                     CPVFSIDTDHSPFYSAPDALAQILCDIAGS"
     gene            complement(9167..9721)
                     /locus_tag="VFF98_09450"
     CDS             complement(9167..9721)
                     /locus_tag="VFF98_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63898.1"
                     /translation="MTAPGLTPELIAFLHEQKDRADILAALHRYTRGVDRHDRALMLS
                     AYWPDAYDEHGVAEGHAAEFVDWALGWHGQYQTRHHHGILNHTCEIDGDTAHSETYYI
                     FWGENREGPAQISFGRYVDRHEKRGGEWRIAHRVCVNEFTADLAATPIPEAWRELMFK
                     SGPNRRDRGDVSYVRPLTRERAPA"
     gene            complement(9850..11145)
                     /locus_tag="VFF98_09455"
     CDS             complement(9850..11145)
                     /locus_tag="VFF98_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU63899.1"
                     /translation="MADHSAAQLPADTAPRRVKTATDHAFDPLDPAVVQNPGPAYSQL
                     AAQCPFYHYEGAEYQFHITSDYKEIRDKILSDNPYWSFKWGDGPADWDSFSDFGILTD
                     PPFHLEYIAALRKGMTPARMRYYLPEVEAIAASLLADMQAAPEKRGNFHDLFALPLPA
                     RTMCFMLGADQALYRDYKRWADALVSFLFNDKDPNAEQALIQEIMPHFMGLIEERRQI
                     LRDAGIDEPGHEHWGTVLPFDYISLGLVSRVEGRRFNDQELFQICTAFLTGGQETTTS
                     LLTNVVWRLLEVPERWEQLKANPDLVDNAIEESLRFDPPINAHFRTSLCPVSMHGTQL
                     PEKSKLMFSMMGANRDPAIFPDPDSFRLDRPLNQSKRHLSFGYGVHFCLGAPIARLEA
                     QVGLRRLVEGLPNLRLLGQPQRIDSWIYWGQRSLPVAWD"
     gene            complement(11223..11756)
                     /locus_tag="VFF98_09460"
     CDS             complement(11223..11756)
                     /locus_tag="VFF98_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZU63900.1"
                     /translation="MPRRPTEQLQAGRAFYANRLPGIDVEHFGALWHLFTLGHLVETD
                     LESIARPMGCSFADLDCLGTLAVDEQRRLRATDLASALYVSDAVMSERIARLHRQGLI
                     ARTPHPDDRRAYTLALTDAGRQLLDRAVEVVAAQAKLVRFLRQLPTEDRQQLVRILGD
                     LHQMYDREFAGQPYFDG"
     gene            complement(11842..13062)
                     /locus_tag="VFF98_09465"
     CDS             complement(11842..13062)
                     /locus_tag="VFF98_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU63901.1"
                     /translation="MQTDTLPHTARPAHVPAELVRDFDLYAIPGSAEDVQAAYAAIQQ
                     ANPDIFWTPHNGGHWVATRGEDIIAMQRDYRHFSHRQIVLPPMPEGTPRQIPLELDPP
                     AHGSYRRPLMQALMPAIVNELEDKVRAVAVAAIERVQPRGACEFIEDFAKVLPIHVFL
                     ELVDLPIADKGILLPMAEESVRGRDAETRLRSQRAMGAYLIDKIRARRDNPGEDLLSK
                     IVNVDIEGSRISEEEAVYYASLVLFGGLDTVAGMLGFVARFLALNPGHRRQLVERLDD
                     EAFLKQAIEELVRRHGLANTARVIPEDFTYKGIAFRAGDRILPANLFVGLDERLNANP
                     LEVDFAREKPVHAAFGNGPHACPGAILARRELRIFLQEWLSRIPDFRLQPGTAPVLAT
                     GMVNGVLRLDLVWP"
     gene            complement(13095..13424)
                     /locus_tag="VFF98_09470"
     CDS             complement(13095..13424)
                     /locus_tag="VFF98_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU63902.1"
                     /translation="MPAISVTLRSGERRQISAADGSTLMEAIRDAGIEEMLALCGGCC
                     SCATCHVQVDLQADPALAAALPPMSEDENDLLDGSAHRGATSRLSCQITLGQPLDGLQ
                     VTIAPED"
     gene            complement(13447..14694)
                     /locus_tag="VFF98_09475"
     CDS             complement(13447..14694)
                     /locus_tag="VFF98_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU63903.1"
                     /translation="MAQDTAVRIEEYDDPTYDPFSSDEINFGDTRDPYPEIARWRAQA
                     PVLEGDYRPRMGLSSALYPDRRMFTVVGTEEILEVLTDTGRFSNAAYRFNIGATFGQG
                     SISTMDNPEHGRWRRIFQKIFLPQYVKHWGESIVDPVVQDLMAAFLPRGAADLVEEFT
                     IRYPFEVIYRQLDLPRADVRTFQRLAIGQTDYMHYDKASEAGRKLGAYFTALVEERRR
                     HPGEDLVSLLANTEVEGEYLPEIVLISFLRQLMNAAGDTTYRGTSVLLTALLENPDQL
                     EAIRQDRALIPQAIEEALRWDGPVAVQLRMASQDVVLGGVQIPAGSLLDVVAGAANRD
                     PAIYPDPDRFDIFREKRAHFAFSRGAHICVGQHLARVEMTRALHAVLDHLPNLRFDPD
                     KPRAEIRGSMMRVPKHIFVRFDA"
     gene            complement(14768..15439)
                     /locus_tag="VFF98_09480"
     CDS             complement(14768..15439)
                     /locus_tag="VFF98_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63904.1"
                     /translation="MPQTDDIAAAHHEAPADQPPARRRRRSREDVVQRIHDAARQLFA
                     EKGFAAATTKEIARLADVSETLLFRYYGDKAGLFGEVVTQPFQQLMDAFVARHPDPTA
                     QGGRLDDTRLFTRQVYGLFEANEGLFRALLAGPGLDEERPHLGGLAPFFAQAVDQVGR
                     RYAAAGQLPPFDLAVGIRLGMGMIAASVLLRDAIFFGLQPDREAVVGALEHFVAHTLA
                     GPSAG"
     gene            complement(15439..>16977)
                     /locus_tag="VFF98_09485"
     CDS             complement(15439..>16977)
                     /locus_tag="VFF98_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU63905.1"
                     /translation="HPACKVLLLEAGPQDRSPFIHMPRGAAKLYTDPRHVWYFQTEAH
                     GDIPAETWIRGKMLGGSSSVNGMMYFRGQPEDYDQWEALGARGWGWRHMGAAFHAMES
                     HEEGGDDTGGDGTGNEGPLRLSQESGRDPFSEAFIAAGEQMGLPRVADLNRPGREGVG
                     YTTRTIWKGRRQSAATAFLQRARGRPNLAILTGALVDKVVFDGVTATGIRAQVQGAWQ
                     HFATHGEVILSAGALMSPQILQRSGIGDAALLARLGIAVRAHSPGVGRHMLEHRLLSM
                     YYDLGRPFSHNPQLRGARLVGNVLRYYLTGKGPMAAGYGTVGAFARVLPESATPDIEI
                     LLSPAVATPDAKGRLAPDAAHSITLFGYPLRSRSEGEVAISAADPGAPAQIRAGYLSD
                     PYDQRVTVAMHRYLRAWMAQPALAAMGLAEREPTRSARSDEEILAAFRQIGQAGYHAC
                     GTCRMGDFADAVLDAKLRVRGVANLRVVDGSIMPTMVSANTNGPIMAAAWRAADLILE
                     GRNS"
BASE COUNT         2665 a         5561 c         6069 g         2682 t
ORIGIN      
        1 gcagcgctat gccgccgagc aggatgcccg cgcgcatctg tccaccggcc cgcgccgcgc
       61 ccgcttccgc caggatatgg cagagcatgg cctgaaggaa gcgctggcca accgcgatgc
      121 gcctttcggc aagggccggg tggaactgcg cgccagggcc ggccgggcgg agtaatgcag
      181 ggcaggggca gcagggaggc ccgctgcccc tgccctgcct agccgcgggc gaggatcacc
      241 gttgccgcgc tggagagcac gccgccattg ccatgggcga tcgccagctc ggcatcgggc
      301 acttgccggg cgcctgcctc gccgcgcagc tggcgcaccg cttccaccag caggaacagc
      361 ccatacatgc ccgggtgcac gcaggagagc cccccgccat tggtgttgag cgcgaggcct
      421 ccgcccggcg ccacatggcc gcccgccaca aagcgcccgc cctcgccctt ggggcagaag
      481 ccgagatctt caagaaacag gatcggcgtg atggtgaagg catcgtagag catggcgaca
      541 tcgatatcgc cgggccccac gcccgcctgc gcaaaggcct gcggccccga gatcgccgcg
      601 ccggtcacgg tcagatcggg catcgagctg atctcgcggt gagtgacggc atgggccgcc
      661 cccagcaccg ccaccggccg gcgcgcgcaa tcgcgcgcgc gctcggcgcg caccatgacg
      721 atggccgccg cgccatcgtt gaacaggcag caatcgcgca cccccagcgg atcggacacc
      781 atccgcgcgg ccagcacatc ctcgatggtg agcggatcgc gcagggccgc ctcggggttc
      841 agccgggccc actggcgcgc ggccaccgcc acttcggcca gctgcgcgcg ggtggtgcca
      901 taaagatgca tgtgccggct ggccgccagc gcataggacg agatcggcgc caacgggcga
      961 tagggcgctt cccaggggct ctggcgggtc atgcccacgg ctggcggcgg gttgctggcg
     1021 gggttggcgc cataggcgat cagcgcgcaa tcgatcagcc cggcctccag cgccagcgcc
     1081 gcctgctgca catagacctc gaaggccgag ccgccggtgc ggttgcaatc cgtgaccctg
     1141 ggggcaaggc ccagatattc ggccagttcc agcccgccca gcgcctcatg cggggcgctg
     1201 gtgtaaagcg catccacctc gccaagggcc atgccggcat cggccagcgc catcagcgcg
     1261 gcgcgcgcgg ccatgtccac cggcgccagg cccgggcagg cgccaatgcc gaaagtggcc
     1321 gcgccaacga tcgcgctgcc cccgcgcgca aagccgcgat ggccgctcat gccggcacga
     1381 agacgagcag cggctggcct tcctcctcga tgatgcgggc tttaacgggc atgccgatcg
     1441 tcaccgcatc ggcggcgatg ccatcgacgc ggctcatcag ccggggcccc tcggccagat
     1501 cgaccagcac gacattatag ctttccgcag ggttgcgccg ccgcaccagg gtggcggcat
     1561 ggatgcgccc ctcgccacag gcgggcaccc attcgagatc ctgcgccccg gtgccgggct
     1621 gggcgatgcg cggatagaac acaaaggccc cgctggcgcg gctgcgctgg agcatgaagc
     1681 ggccggcggc cagatgggcg cgccattcgg cctcggggtg gggcaggggc gggggctggc
     1741 tcatcgcgct cattcccccc tgaacgccgg cgggcgcttt tcgcggaagg cgcgcagccc
     1801 ttcctcatgg tcctcggtgc ggctggcgat gggctgcatg gtggcttcca tgtcgagata
     1861 ttgctcgaag ccggcgttcc acgagaggtc gaactggcgc ttggccaggc ccatcgccac
     1921 ggtgggcccg gtggcaaagc tggtggccag ttccagcgcg cggtccatca gcgtggcatc
     1981 ggccaccttt tccagcgcga tgcccagcgc cacggcttcc tcggcgcgga cgatgcggcc
     2041 ggaaaagacc agctcccttg cccgctgcac gcccatcagc tggcgcaagt gccacaccag
     2101 gccgccatcg ggcgccagcg cgatgttgcg gaagatggcg gcgaatttgg ccttctcgcc
     2161 ggccagcacg atgtcgcagg ccagcgcata ggaaaagccc acccccacgc acacccccgg
     2221 caccgccgcg atggtgggct tcttcagccc gtggatcgcg cgggcgatgc ggttgagcgt
     2281 gtgcatccgg tcgatcgatc cggtgatgcc gccgcgcccc atggcgccca catccatgcc
     2341 cgagcagaaa tcgcccccgg ccccggtgag gatcaccgcg cgcacctcat gatcgagcgc
     2401 gatatcctcg aaagcgccat agagctggcc gcgcatgtcg tgggtgatgg cgttcttggt
     2461 ctcgggccgg tcgagcgtga cgatggccac ggggccctgc cgggcaaggg tgatggtcat
     2521 gggcgatagg gcctcgcata tgtgtgaaaa gggatggccc gatgatgtgt tggctggcgc
     2581 ccggccggtc aagcaaggcg gaaaacatcg gaaacgtgat gagctggccc gccccctggc
     2641 tgcccccttg gcaagccctg ccggcgcctt ctaactcgcc cgaggacagg gagtttcacc
     2701 catgaaggaa gcggccatcg tggccaccgc gcgcacgccc atcggcaagg cgcatcgcgg
     2761 ctcgctcaac aatatccacg gcgcctcgat ggccggccat gtgatcgccg cggccgtgcg
     2821 ccgcgccggc ctcgatccgg ccgagatcga ggatgtgctg ctgggctctg cccgctgcga
     2881 gggggcggtg ggatcgaaca ttgcccgcca ggcggccctg cgcgcggggc tgccgatcag
     2941 cacctgcggg gtcacgctcg atcgcaaatg cgcctcgggg ctgcaggcca ttgcctttgc
     3001 cgcccagcgc atccgcgccg gcgagggcgg catttatgtg gccggcgggg tggaaacggt
     3061 gagcctggtc gatccccatc gcaacacctt ccgcaacaag gacgagtggc tgctggccca
     3121 ccagcccgag atctacatgg cgatgaaccg caccggcgat atcgtcgcgc agcgctatgg
     3181 catcagccgg gcagaccagg atgccttcgc gctggccagc cagagccgca ccgccgccgc
     3241 ccagcaagcg ggcgccttcg acgaggagat cgtgccgatc gacgtgatcc gcaatgtggt
     3301 ggacagggca ttggccgtgc agggccagga agcggtgacg ctggcgcgcg atgaatgcaa
     3361 ccgcccgggc accgaactgg ccgcgctggc gcagctcaag ccattgttcg agggcggcac
     3421 ggtgacggcg ggcaattgca gccagctgtc ggatggcgcc agcgtgtgcg tggtgatgga
     3481 tgccgacgag gcggcccggc gcggccttcc catcctcggc ctctatcggg gcatgcaggt
     3541 ggccggctgc gcgcccgacg agatgggcat cggcccggtt cttgccgtgc cgcgcctgct
     3601 ggagcggctg ggccttgcgg caggagacat cgacttgtgg gaactgaacg aggcctatgc
     3661 cagccaggcg ctctattgcc agcggcggct gggcctggat ccggcgcggg tgaatgtgga
     3721 tggcggggcg atcgcccttg gccacccctt cggcatgacc ggctcgcgca tggccggcca
     3781 cgccctgctg gcggggcggc ggcgcggcgc gcgccatgcg gtggtcacca tgtgcattgg
     3841 cggcggcatg ggggccgcgg ccctgttcga gatcgtctcc tgagcgcggg gcggcgagat
     3901 ccttcacaag cctgatggat tggcgagttt cacccggccc tgcttgccct ttccgccagg
     3961 cgcagggcaa tgtcgggcca atggcccctg cggctgggcg cggatggctg ggagagagtg
     4021 catgagtgtg ggaaatctgg acccgacggc ggccgagcag ctggacatgt atcgccggat
     4081 ggcgctgatc aaggcgaatg acgaacgctc gcgcaaggtg atcatgagcg gccggctggt
     4141 gatgccctat tacagcccgc gcgggcagga tgtgatcccc agcgccgtca gcgtgaacct
     4201 taccgacgac gactatatct gcaccatcta tcgcggcacg catgacatgc tggccaaggg
     4261 ctttccgctg aaggacatgt gggccgaagt ggccggccgc accaccggca cctgcaaggg
     4321 caagggcggg ccgatgcatc tgacctgccc ggaaaaaggg gtgatggtga caaccggcat
     4381 cgtcggctcg tcgatgccga tcgccaatgg catcgcctgg ggctcgcagc tgaagggcga
     4441 tggccgtgtg acggtggcct atttcggcga tgcggcggcc aatatcggcg ccttccacga
     4501 aagcctgaac ctggccgcgg tgtggaagct gccggtggtc ttcgtctgcc agaacaatga
     4561 atggggcgag cataccgcct atgagaagac cagcaacgtg cgcgtggcag accgggcggc
     4621 cgcctatggc atgccgggcg agcgggtgga tggcaatgat cccttcgccg tctatgccgc
     4681 tgcgcgccgg gcggtggcgc gcgcgcgggc gggcgatggg cccagcctga tcgaggcgat
     4741 gacctatcgc ttccacggcc acgtcttcgg cgatcaggat gcctatatgg acaaggcccg
     4801 caaggccgat gcgatggcca atgatccggt gctgcgcttc cgtgcccggc tggtggaaag
     4861 cggcattgcc ggcgaggacg agctggccgc catcgaggcg gccatcgagg cggccatcga
     4921 cgaggcggtg gactatgcgc tggccagccc cttcccggat gtggccgaac tgaagcgcga
     4981 tgtgtttgcc gaggagctgg cgtgatggcc gtgagcgaaa agaccaaacc tgctgccgaa
     5041 ggcgccgccg cgcggcctgc aaaagtgaac atcctgcagg cgatcaacgc cgccatggcc
     5101 gatgccatgg ccgccgacga cagcgtgctg gtgctgggcg aggatgtggc agacccggag
     5161 gagggcggcg tgtgcggtgt cacgcgcggg ctgtccagcc gctttggcga tcatcgcgtg
     5221 cgctcaacgc cgatttccga acaggcgatc attggcgcgg cgattggcgc ctcgctggtc
     5281 ggcttccggc cggtggccga gatcatgctg atgaatttca ccaccgttgc catggacatg
     5341 atcgtcaacc acgcggccaa gctgcgcttc atgtcgggcg ggcagacgca tgtgccgatc
     5401 gtcatccgca cgatgaccgg caccggcttt gcctcgggcg gccagcattg cgactatctg
     5461 gaggcctggt tcgcccacac cgccggcctc aaggtggtgg cgccgtccag ccccagggat
     5521 gcctacgggc tgatgcgcag cgccatcgag gaccccgatc cggtgctgtt catcgagaac
     5581 ctgccgacct actggctggc cgacgaagcg cccgagagcg gctatcgcgt gccgatcggc
     5641 aaggccaatg tgctggccga gggcagcgat gtgacgatca tcgcctatgc ccggatgatc
     5701 caggaagcgg cgcccgccat cgcccggctg gccgaggccg gcatttcggc cgagctgatc
     5761 gacctgcgct cgattgcccc gtgggaccgc gcggcagtgc tcgcctcggt cgcgaagacc
     5821 ggccgggcga tgatcgtgca cgaggcggtg accccctttg gcgtgggcgc tgaaattgcc
     5881 gccacgctga acgaggaact gttcggccgg ctgaaggcgc cggtgaagcg gctgggcggg
     5941 gcgttctgcc cggtgccctt ctccaagccg ctggaaaccg cctttgcccc ccagaccgag
     6001 gacatcttcg ctgcagcctg cgcgctggtg tctgcctgat cccttttgcc gacggagtat
     6061 tgcatggcca ccgaagttct tctgcccaag ctgggttttt ccatgaacga gggcacgctg
     6121 gccgaatggc tggtggccga tggcgggcag gcggtggagg gcgagcccct ttatgcgctg
     6181 gaatcggaga aatcgacgca ggaagtggag agccccgcct cgggcacgct gaagatcatc
     6241 gcgcagatcg gcgagaccta tgaggttggc accgtgctgg cggtgatcgg gtaagcggca
     6301 tggcacgcta cacgctggaa gcctgctcgc gcgcgctggc cgggcgcgag gcggaatatg
     6361 cccactggta tcgcgatgtg catctggccg aggtggtggc cctgccgggc ttcctctcgg
     6421 gccggctgca ccagcggctg gatgcgcaag gggcgcaggc gggcgagctg gtcgcccatt
     6481 atgcggtgga aacagatgcg cccgcggcct tgctggaagc gctgtttgcc gccgcccccg
     6541 ccatgcggct gaccgacagc atcgacatgg ccagcctgcg tttctccttc ctggcagagg
     6601 cggcgtaatg gccggcctgc tcgatgggct ggccccttgg ccggcggtgg atttcagcgc
     6661 cttcgggccg gtggaaacgc agcctctcag caaaatccag gcctatgtgg cggcgcatct
     6721 tggccgcaac tgggtggcga ttccgcatgt cacccatcag gacgaggccg atgtcaccgc
     6781 gctggaggaa cagcgccgct cgctggccgc tgccggcggg cccaagctga ccccggtgcc
     6841 actgctggtg aaggcgctgg cgcgggcgct ggcggctttt ccgcgcttca acgcctcgct
     6901 cgatccggcc agcggccagc tggtactcaa gcgctatgtc catgtcggcg tggcggtgga
     6961 tacgcccggc gggctgctgg tgccggtgat ccgcgattgc gatgccaaga gcgccgctgc
     7021 catcgccacc gaactggccg ccctttccga aaaggcgcgc accaaggggc tggccatggc
     7081 ggagatggcg gggggctgca tcacgctcag ctcgctgggc catattggcg gcaccggctt
     7141 cacgccgatc atcaatgcgc cggaagtggc gatcctgggc gtggcgcggg cgcgggcggt
     7201 ggcgcagccg gccgccgatg gcagcctggc ctggcggcag atgctgccgc tgtcgctctc
     7261 ctatgatcac cgggtgatca acggcgccga tgccgcccgc ttctgccgct tcatcgccga
     7321 tcagctggcc gatcccgccc tgttcgactg aacgggcggg cgggcgccag gcgggccggc
     7381 ctcaagcatc gcccgggcga tagagccgcc cgagcgtgcc ggccagcggg gtgggcacga
     7441 aaccatcgcc aaagggccag tcgaggaagc cggcggcggc catcgtgccg ccatcgacat
     7501 gcagcgtggt gcccgagacg aagcgcgaca ggtcggaggc gaggaacagc accgcatcgg
     7561 ccagcgcctc tatcggcggg gcctcgccgc gcgggatata catctccagc cccttgcccc
     7621 gcgcggcttg cggcagggcg gcgaagcggg cggcaaaatc ggccggcatg gcggccagat
     7681 tgccggcgct gggggtggtg tcgggagcga tgcaattggc gcggattttc tccttgccca
     7741 gctccaccgc gatggcgcgg gtgaaattgg cggtggccgc cttggccccg gcataaacgg
     7801 caaagccggc cgcgccccga tgcgcctcga tggtggtgaa attgacgatg ctgcccccgt
     7861 cgccagaacg gcgcagcagc ggcagggccg cctgcaccga ttgcaccaca tagccatagt
     7921 tgcggtggat atcgcgggcg atatcggcgc ggctggcatc ggccagcggc ccgcgccggg
     7981 tgccgccggc aaggttgacg aggatatcca gccgcgggaa ggcgccggca agatcggcat
     8041 agaagccatc cagcgcggcc tcgcaggtgg catcggcgtg gcaggccagc acctggcggc
     8101 ccagcccttg cgcgcgcgcg gccgtggcgg ccagggcgcc cgcgtcgatg tcgcacaggg
     8161 ccagatccac ccccgcctcg gccagggcca gcgccacgcc cgcgccaatg ccgccgccgc
     8221 cgccgatcac tgccgccacc ttgccggcaa gccctgcccg cccatccaga aatccgccca
     8281 tatcgtgcct ctgttattgt gtcggccggt gttgcaggca aagctgcggg cgggcaatcg
     8341 agcggccgca agccatcacg gggctgatgg cgcggcaagc agggggaggg cacgggcatg
     8401 gcgcgtttca ttctggtgca tggggcctgg catggcggct ggtgctggga agagatcgtg
     8461 ccgcggctgg aagcggccgg ccacgaggct ttggcgcccg atctgccggg catgggcgcc
     8521 gatgccgaca gcggccatgt ggccagcctg gaggaatggg cgatgttcat cgccggcgcg
     8581 gcggcggccg gcggcggggc ggggcggcag ccggccattc tggtgggcca cagccggggc
     8641 ggcatggtga tcagccgggc ggcacagctg gcgccggtgg gcagcgtggc ccatctggcc
     8701 tatctggccg ccgccatgcc gcgcccgggc cagacgatgt cggaactgtt cgatgcggcc
     8761 gcgctctcgc gcatggtcga catgaccggg gcgatcgccc gctcggccga tgggcgccag
     8821 ctgatcttcc acgatgccgc caccgcgctc agggcctttt acggcctgtg cccggccggc
     8881 aagggcgagg ccgccttcgc ccggctgacg cccgagcccg ccggcatccg caccgcgcgg
     8941 gtggagctgg acgcggcccg ctatgggcaa gtggcgcgca gctatatccg ctgcgaaagg
     9001 gacatggcgg tggtgcccga attgcagaac cgcatgatcg ccgcccagcc ctgcccggtg
     9061 ttcagcatcg acaccgacca ttcgcccttc tattccgccc ccgatgcgct ggcgcagatc
     9121 ctgtgcgata tcgccggaag ctaggccaga ggttgcaggc ggggcgtcag gccggcgccc
     9181 gttcgcgggt caaggggcgc acataggaaa catcgccgcg atcgcgccgg ttggggccgg
     9241 atttgaacat cagctcgcgc caggcctcgg ggatgggcgt ggcggccaga tcggcggtga
     9301 attcattgac gcagaccctg tgggcgatgc gccattcgcc gccgcgcttc tcgtgccggt
     9361 cgacatagcg gccgaagctg atctgcgcgg gcccctcgcg gttttcgccc cagaagatgt
     9421 agtaggtctc gctgtgggcg gtgtcgccgt cgatctcgca ggtgtggttg aggatgccgt
     9481 ggtggtggcg ggtctggtat tggccgtgcc agcccagcgc ccagtcgacg aattctgcgg
     9541 catggccctc ggccacgcca tgctcgtcat aggcatcggg ccaataggcc gacagcatca
     9601 gcgcgcggtc gtggcggtcc accccgcggg tatagcggtg cagcgcggcg aggatatcgg
     9661 cgcggtcctt ctgctcgtgg aggaaggcga tcagttccgg ggtgaggccg ggtgcggtca
     9721 tcggggggct ctcctgtcgg gcgcgtgtgc ttggtttggc cgtgatgatg gcccggccgg
     9781 cgccccgcac caagagggcg ccggccggcc gggcagattt cagataggcg atatgggccg
     9841 aaggcgggct cagtcccacg ccaccggcag gctgcgctgg ccccagtaga tccagctgtc
     9901 gatccgctgt ggctggccca gcaggcgcag gttgggcagg ccttccacca gccggcgcag
     9961 gcccacctgc gcttccagcc gggcaatcgg cgcgcccagg cagaaatgca cgccatagcc
    10021 gaagctgaga tgccgcttcg actggttgag cggccggtcg aggcggaagc tgtcgggatc
    10081 ggggaagatg gcgggatcgc ggttggcccc catcatcgag aacatcagct tggatttttc
    10141 cggcaattgc gtgccatgca tcgataccgg gcacaggctg gtgcggaaat gggcgttgat
    10201 cggcggatcg aagcgcaggc tttcctcgat cgcattgtcc accagatcgg ggttggcctt
    10261 cagctgttcc cagcgttcgg gcacttccag cagccgccac acgacattgg tgagcaggct
    10321 ggtggtcgtc tcctgcccgc cggtgaggaa ggcggtgcag atctggaaca gttcctgatc
    10381 gttgaagcgc cgcccttcca cgcgtgagac aaggcccagc gagatatagt cgaacggcag
    10441 caccgtgccc cagtgttcat ggccgggctc gtcgatgccg gcatcgcgga ggatctgccg
    10501 gcgctcctcg atcagcccca tgaaatgcgg catgatctcc tggatcagcg cctgttcggc
    10561 attgggatcc ttgtcgttga acaggaagga gaccagcgca tcggcccagc gcttgtagtc
    10621 gcggtagagc gcctgatcgg cgcccagcat gaagcacatg gtgcgcgcgg gcagcggcag
    10681 ggcgaacaga tcgtggaaat tgccccgctt ttcgggggcg gcctgcatgt cggccagcag
    10741 gctggcggcg atcgcttcca cctcgggcag gtagtagcgc atgcgggcag gggtcatgcc
    10801 cttgcgcagg gcggcgatat attcgaggtg gaagggcgga tcggtgagga tgccgaaatc
    10861 gctgaaactg tcccagtcgg ccgggccatc gccccatttg aacgaccagt aggggttgtc
    10921 ggagaggatc ttgtcgcgga tctccttgta atcgctggtg atgtggaact gatattcggc
    10981 gccttcataa tgatagaagg ggcattgggc ggccagctgc gaataggccg ggcccgggtt
    11041 ttgcaccacg gccggatcga gcggatcgaa ggcgtggtct gttgcggttt tcacgcggcg
    11101 gggggcggtg tctgcgggca gctgcgctgc gctgtggtct gccatggcgg tctcccttgg
    11161 tgctgttgca gggcagctta ggcaggcctg gccccgggga gaatgcgcag atgttcaggg
    11221 ccctaaccat cgaaataggg ctggccggca aattcgcggt catacatctg gtgcagatcg
    11281 ccgaggatgc gcaccagttg ctggcggtcc tccgtcggca gctggcgcag gaagcgcacc
    11341 agcttggcct gcgccgccac cacttccacg gcccggtcca gcagctggcg gccggcatcg
    11401 gtcagcgcca gcgtgtaggc gcggcggtcg tcggggtggg gggtgcgcgc gatcagcccc
    11461 tgccggtgca gccgggcgat gcgttccgac atcaccgcat ccgagacata cagtgccgag
    11521 gccagatcgg tggcgcgcag gcggcgctgt tcgtctaccg ccagcgtgcc caggcaatcg
    11581 agatcggcaa agctgcaccc catcggccgg gcgatgcttt ccagatcggt ttccaccaga
    11641 tggcccagcg tgaacaggtg ccacagggcg ccgaaatgct cgacatcgat ccccggcagg
    11701 cggttggcat aaaaggcgcg gcccgcctgg agctgctctg tgggcctgcg cggcatggtg
    11761 tgttctcccg gcgccttgcc cgccggcgcc tcttgcgggg caccctgcgg gcaagggatg
    11821 tgcccggtta tgcgcgcgcg ctcagggcca gacaagatcc agccgcagca ccccgttcac
    11881 catgcccgtg gccagcaccg gcgcggtgcc cggctgcagg cggaaatcgg ggatgcggct
    11941 cagccattcc tgcaggaaga tgcgcagttc gcgccgggcc aggatagcac ccgggcaggc
    12001 atgggggcca ttgccaaagg cggcatgcac gggcttttca cgggcgaaat ccacctccag
    12061 cgggttggcg ttcagccgct cgtccagccc cacgaagagg ttggccggca ggatcctgtc
    12121 gccggcgcga aaggcgatgc ccttgtaggt gaaatcctcg gggatcacgc gggcggtgtt
    12181 ggccaggcca tggcggcgca ccagctcctc gatcgcctgt ttgaggaagg cctcatcgtc
    12241 cagccgctcc accagctggc ggcgatggcc ggggttgagc gcgagaaacc gcgcgacaaa
    12301 gcccagcatg ccggccaccg tatccagccc gccaaacagc accagcgagg cataatagac
    12361 cgcctcctcc tcgctgatgc ggctgccctc gatatcgaca ttgacgatct tgctgagcag
    12421 gtcctcgccc ggattgtcgc ggcgggcgcg gatcttgtcg atcagatagg cgcccatcgc
    12481 ccgctgcgag cgcaggcgcg tttcggcatc gcgcccgcgc accgattcct cggccatcgg
    12541 cagcagtatg cccttgtcgg cgatcggcag gtccaccagt tcgaggaaga cgtggatcgg
    12601 cagcaccttg gcgaaatcct cgatgaattc gcaggcaccc cggggctgca cacgctcgat
    12661 cgcggccacc gccaccgcgc gcaccttgtc ttccagctcg ttgacgatgg ccggcatcag
    12721 cgcctgcatc agcggccggc gatagctgcc atgggcgggt ggatccagct ccagcgggat
    12781 ctggcgcggg gtgccctcgg gcatgggggg cagcacgatc tggcgatgcg agaaatgcct
    12841 gtagtcgcgc tgcatggcga tgatgtcctc gccgcgggtg gccacccagt ggccgccgtt
    12901 gtgaggggtc cagaagatgt cgggattggc ctgctggatg gcggcatagg cggcctgcac
    12961 atcctcggcg gagccgggaa tggcgtagag atcgaaatcg cgcaccagct cggccggcac
    13021 atgcgcgggg cgggcggtgt ggggaagggt gtccgtctgc atgggaaatt cccttgatga
    13081 tggggaagag ggcctcagtc ttcgggcgcg atcgtcacct gtagcccgtc gagcggctgg
    13141 cccagcgtga tctggcacga caggcgcgag gtggcgccgc gatgggccga accgtcgagc
    13201 aggtcgttct cgtcctcgct catgggcggc agggcggcgg caagggcggg gtctgcctgc
    13261 aggtccacct gcacatggca ggtggcgcag gaacagcagc cgccgcacag ggccagcatc
    13321 tcctcgatgc cggcatcgcg gatggcttcc atcagcgtgc tgccatcggc ggcgctgatc
    13381 tggcggcgct cgccgctgcg cagggttacg gaaatggcgg gcatctttct ctccggtatc
    13441 gggtgttcag gcgtcgaagc gcacgaagat gtgtttgggc acgcgcatca tcgatccgcg
    13501 gatctcggcg cgcggcttgt cgggatcgaa gcgcaggttg ggcaggtgat cgagcacggc
    13561 atggagcgcg cgcgtcatct ccacccgcgc cagatgctgg cccacgcaga tatgcgcccc
    13621 gcgcgagaag gcgaaatggg cgcgtttttc gcggaagatg tcgaagcggt cggggtcggg
    13681 gtagatcgcc ggatcgcggt tggcagcgcc tgccaccaca tccagcagcg atccggccgg
    13741 gatctgcacc ccgcccagca ccacatcctg gctggccatg cgcagctgca cggccaccgg
    13801 cccatcccag cgcagggctt cctcgatggc ctgggggatc agggcgcgat cctggcggat
    13861 ggcctcaagc tgatcggggt tttccagcag cgccgtcaac agcacactgg tgccgcgata
    13921 ggtggtgtcg ccggccgcgt tcatcagctg gcgcaggaag gagatcagca cgatctcggg
    13981 cagatattcg ccctccacct cggtgttggc cagcaggctg acgagatcct cgccggggtg
    14041 gcgccgccgt tcttccacca gcgcggtgaa ataggcgccc agcttgcggc cggcctcgct
    14101 ggccttgtcg tagtgcatgt agtcggtctg gccgatggcc aggcgctgga aggtgcgcac
    14161 atcggcgcgc ggcaggtcga gctggcggta aatgacctcg aagggatagc ggatggtgaa
    14221 ttcctccacc agatcggcag cgccgcgcgg caggaaggca gccatcagat cctgcaccac
    14281 cggatcgacg atgctttccc cccagtgctt cacatattgc ggcaggaaga tcttctggaa
    14341 gatgcgccgc cagcggccat gctcggggtt gtccatggtg ctgatgctgc cctggccgaa
    14401 ggtggcaccg atgttgaacc tgtaggcggc attggagaag cgcccggtat cggtgagcac
    14461 ttcgaggatc tcttcggtgc cgacgacggt gaacatgcgc cggtcgggat agagcgcgga
    14521 ggacaggccc atgcgcgggc gatagtcgcc ctccagcacg ggggcctggg cgcgccagcg
    14581 ggcgatctcg gggtaggggt cgcgggtgtc gccgaaattg atctcgtcgg agctgaaggg
    14641 gtcgtaggtg gggtcgtcat attcctcgat ccgcacggcg gtgtcctgtg ccatctgcct
    14701 ctccttatgt agtgatcgct acataacttt gccggagcgg gcctgtcaat gcgcacaggc
    14761 ccccgcgtca gccggccgac ggcccggcca gcgtatgggc aacgaaatgt tccagcgcgc
    14821 ccaccaccgc ttcccggtcg ggctgcaggc caaagaagat ggcatcgcgc agcagcacgc
    14881 tggccgcgat catgcccatg cccagccgga tgcccacggc cagatcgaac ggcggcagct
    14941 ggcccgccgc ggcatagcgg cgccccacct gatccaccgc ctgggcgaag aacggggcca
    15001 gcccgcccag gtgagggcgt tcctcatcga ggcccgggcc tgccagcaac gccctgaaaa
    15061 gcccttcatt tgcctcgaac aacccataga cctggcgggt gaacaggcgg gtgtcgtcca
    15121 gccgcccgcc ttgcgccgtg ggatcggggt ggcgggccac gaaggcatcc atcaactgct
    15181 ggaagggctg ggtcaccact tcgccgaaca ggcccgcctt gtcgccgtaa tagcggaaca
    15241 gcagcgtctc gctcacatcg gcgaggcggg cgatttcctt ggtggtggcc gccgcaaagc
    15301 ccttttcggc gaacagctgc cgcgcggcat catgtatgcg ttgaacgaca tcctcgcggc
    15361 tgcgccggcg gcggcgggcg ggcggctggt cggccggggc ctcgtggtgg gcggcggcga
    15421 tgtcgtcggt ctggggcatc aactgttgcg tccttccagg atcagatcgg ccgcgcgcca
    15481 ggcggcggcc atgatcgggc cattggtgtt ggccgaaacc atcgtgggca tgatggatcc
    15541 atcgaccacc cgcaagttgg caacgccgcg cacgcgcaat ttggcatcca gcacggcatc
    15601 ggcaaaatcg cccatgcggc aggtgccgca ggcgtgatag ccggcctggc cgatctggcg
    15661 gaaggcggcc aggatctcct cgtcgctccg ggcagagcgg gtgggctcgc gctcggcaag
    15721 gcccatcgcg gccagcgccg gctgcgccat ccaggccctg agatagcggt gcatcgccac
    15781 ggtcacccgc tggtcgtagg gatcgctgag atagccggcg cggatctgcg cgggggcccc
    15841 gggatcggca gcgctaatcg ccacctcgcc ttccgagcgc gagcgcagcg gatagccgaa
    15901 cagggtgatg ctgtgggcgg catcgggcgc caggcggccc ttggcgtcgg gcgtggccac
    15961 ggcgggcgag agcaggatct cgatatcggg cgtggccgat tcgggcagca ccctggcaaa
    16021 ggcccccacc gtgccatagc ccgccgccat cggtcccttg ccggtgagat aatagcgcag
    16081 cacattgccc accagccggg cgccgcgcag ctgcggattg tggctgaagg gccggcccag
    16141 atcgtaatac atcgacagca gccggtgttc cagcatgtgg cggcccacgc cggggctgtg
    16201 ggcccggacg gcgatgccaa ggcgggccag cagcgcggca tcgccaatgc ccgatctttg
    16261 caggatctgc ggcgacatca gcgcgccggc gctgaggatc acttcgccat gggtggcaaa
    16321 gtgctgccag gcgccctgca cctgcgccct gatgccggtg gccgtcaccc cgtcgaacac
    16381 caccttgtcg accagcgcgc cggtgaggat ggcgaggttg ggccgcccgc gcgcgcgctg
    16441 caggaaggcg gtggccgcgc tttgccggcg gcctttccag atcgtgcggg tggtgtagcc
    16501 cacgccttcg cggcccgggc ggttgagatc ggccacgcgt ggcaggccca tttgctcgcc
    16561 ggcggcgatg aaggcttcgg agaaaggatc gcgcccgctt tcctgcgaca ggcgcaacgg
    16621 gccttcgttg cccgtgccat cgccgcccgt gtcatccccg ccctcttcgt ggctttccat
    16681 cgcgtggaag gccgcgccca tgtgccgcca gccccagccg cgcgcaccca gcgcttccca
    16741 ctggtcgtaa tcttccggct ggccgcggaa atacatcatg ccgttgaccg aggacgagcc
    16801 gcccagcatc ttgccgcgga tccaggtttc ggcgggaatg tcgccgtgcg cctcggtctg
    16861 gaaataccag acatggcgcg ggtcggtgta gagtttggcg gcgccgcgcg gcatgtggat
    16921 gaaggggctg cggtcttgcg ggccggcttc cagcagcagc accttgcagg ccggatg
//
LOCUS       DASRYE010000181         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_152059,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000181 DASRYE010000000
VERSION     DASRYE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152059"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFF98_09490"
     CDS             complement(<1..569)
                     /locus_tag="VFF98_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63906.1"
                     /translation="MGAICLAAVLALAPAGPALAHGTYGEDLLAGSLRHYPAVLGLRI
                     LATDRSGAAIALAEGDFGQGLAILLPLANAMGEPVGELTVQCRCSAGQARRVAAHLAR
                     RILTAEGLSEPAPFVAGAQTAPAAQAIVEREVDRHPALVTLAMHVARPQGANAIIASS
                     FGRIGKAADKDDAHVIADGAILREVTNGG"
     gene            827..2212
                     /locus_tag="VFF98_09495"
     CDS             827..2212
                     /locus_tag="VFF98_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU63907.1"
                     /translation="MAHARPLRPRRHGARASAAALLLALAGCAAYRPAPLPDSQLALA
                     PPVAAVMSTQAEHIDRPWLHPATIDLARPLDANAIGVLAVLANPDLKAQRAAAGISAA
                     QAFAARLLPDPNLSFSYDRLLSGPDSLDNFAGQIAQDIGLLRERKVIAAAGRAQNRQV
                     RLDIAWAEWQTAGAARLQAVRVAALETQVTLARSSDTSARAMLASSLGASLRGDIAGD
                     AVQANRLAALDAAERLRAAETALAAARQELARLLGLPPGSRLQLADTAPAVPAELDPA
                     RLFALAERNRTDLAALRAGYAAQEAAVHKAVLDQFPTLQLGFGASRDTTGNQLLGSSV
                     NFTLPLWNRNRGGIAIETATRASLQAAYVARLAQTRADIAAAIDGIALARHTASDLQS
                     QIAALSAYAMGASRAAAHGDLAATTALLARQSLLDRQSQLSAANQALGEQMIALELLT
                     GTPISSWNQTS"
     gene            2209..3261
                     /locus_tag="VFF98_09500"
     CDS             2209..3261
                     /locus_tag="VFF98_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63908.1"
                     /translation="MTQRWICAAFTLLAACSASDSEKTSEPVALVQLAAARPGTLGDA
                     LALYGAVEPGAGSERTLSAPTEAIVTAITAPAGTGVSAGALVLQLNASPQSALDLAKA
                     RSDGAAADAALARALRLKADGLVGNAEVEAARAAASAADATLASLRARAGQLAMRAPV
                     GGVVDAVLVKPGDLVAAGAPLARIATGRAVRARFGIDPALSAAIPPGARITITAGAAA
                     LTVPVSGVDRVVDPATRLAALFANLPAGAQMALGAPLAGTLQRPGAKRGTLIPYAAVQ
                     DDGGTPFVFVVQGGVAHRREVSLGAQASGQVLVTAGLAPGEQVVTQGGTALDDGMKVR
                     TGPIADEKADEKGAKE"
     gene            3258..6353
                     /locus_tag="VFF98_09505"
     CDS             3258..6353
                     /locus_tag="VFF98_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU63909.1"
                     /translation="MNRFLKRFDKAIWLALALLTLGGLIAAWKLPVGLFPHIDYPRIL
                     VSIDAGERDPQAMQAQITRPLEIALRAVPGVTQIRSTTSRGAADIALSFDWGEDMVAA
                     TLATQGALAGVQPDLPAGTRFAVRRSDPTIFPVIGLALTSKSADPKALRQFADLRLRP
                     LLLTVPGVAGIDVLGGAAREVQVEIDPGHLQALGLTMGDVVQALSAANSVRGVGRIED
                     RHRLYLVLVDDRLKNPGDVGAIPIKAGSPAAGTRGNAGVVTLGQIARITPSSPPQWTR
                     VTSNGTDAVLVNIRQSLTGDTVAIVKAINARLAAEHIPAAITIATFYDQSELVAGAAG
                     AVRDAIVLGALLAGLVLFFFLRSGRLMLITGLMLPAVLAATCLVLAMLGQSFNMMTLG
                     GLAAAVGLVVDDAVVMLEHIMRRMMGEESTGPDQLLTAAGEMSRPLLGATLATAVVFM
                     PLAFISGVSGGFFKALAVTMVAALTISLAYARFVIPLATRRWLRAKDARAAERADGMM
                     AWLGRHYARACRQALARPLLVVGATALVLGGAGLLAFSRVPSGFMPAMDEGGFILDYK
                     AHPGAALSDTDHILRQVEAIIRATPEVASYSRRTGAQLGGGLSEADEGDFFIRLKDGS
                     RRPIDDVMAEIRQRVQHEVPGIDIETAQLMEDLIGDLTAVPQPIEIKLFSDNPEELTG
                     AARKVARAIGQVNGVVEVVDGLRVAGDAILVKVDRAAAEAQGLDPDAVASQLETQIGG
                     TVATQYQLGEQIIGVRVRGPADQRQRADQLAAMQLRAPDGHLVSVRQIAAVTVNAGER
                     QLTREDLAPFVAVTARLSGRDLGSGMGEIKGVITGLNLPPSVRVEYGGLYAVQQASFI
                     DLAGVLAAAFLLAALLLTILFNRWRYTIGVMATVLLSVAAVLLGLWMTGIELDISALM
                     GLTMVVGMVSELAIFYLFELDSAAAAHGDAGGLLEAGLGRLRPILMSALIAILTLAPL
                     ALGLSRGAGLQQPLATAIIFGLTVGAPLVLLFLPAMLRIGIAARPLPDDRLLSAMTIK
                     P"
BASE COUNT          926 a         2360 c         2198 g          983 t
ORIGIN      
        1 ctgccgccat tggtcacttc ccgcaggatc gcgccatcgg cgatgacatg ggcatcatcc
       61 ttgtcggccg ccttgccgat ccgcccgaaa ctggaagcga tgatcgcatt ggccccctgc
      121 ggccgcgcca catgcatggc cagcgtgacc agcgccggat ggcgatcgac ctcgcgctcg
      181 acgatcgcct gcgctgcggg cgctgtctgc gcgccggcca cgaaaggcgc cggctcgctc
      241 agcccctcgg ccgtcaggat gcggcgtgcc agatgggccg cgacgcgccg ggcctgcccg
      301 gccgaacagc ggcactgcac cgtcagttcg ccaaccggct cgcccatggc attggccagc
      361 ggcagcaaga tcgccaggcc ctggccaaaa tcgccttctg ccagcgcgat cgccgcgccg
      421 gatctgtcgg tcgccagaat acgcaggcca agcaccgcgg gatagtgccg caggctgccc
      481 gccagcagat cctcgccata tgtgccatgg gccagcgccg gccccgctgg cgccaaagcc
      541 agcacggcgg caaggcatat cgcccccatc tgcatgaacc gctccctttt ttcgcgtcat
      601 tcaacgggcc tgcgataggc gcgatccctg tcgttcaact gacacgggcg cccaactgac
      661 atcgcacatt tgcccgcaca acttcatggc gccggccatc tgcgacagca tggcgtcagc
      721 ttcaacaggc ataggcagcg ggaaacaggc tgacctttca aggcctgaag aggaatgcgc
      781 tgatggttca tccgatgttt cgcgcggcaa caggggcatc tgcctgatgg cccacgcccg
      841 ccccttgcgc ccacgccggc atggcgcccg ggcttcggcc gcagcgctgc tgctggcgct
      901 ggccggctgc gccgcctatc ggccagcgcc gctgccagac agccaactgg ccctggcccc
      961 gcccgtggcc gccgtgatga gcacccaggc cgagcatatc gaccgcccct ggctgcaccc
     1021 cgccacgatc gaccttgccc ggccgctgga tgccaatgcc attggcgtgc tggcggtgct
     1081 ggccaacccg gatctgaaag cccagcgcgc cgctgccggg atcagcgcgg cccaggcctt
     1141 tgccgccagg ctgctgcccg atccgaacct cagcttctcc tacgaccggc tgctgagcgg
     1201 ccccgacagt ctcgacaatt tcgccggcca gatcgcgcag gacatcggcc tgctgcgcga
     1261 acgcaaggtg atcgcggcgg ccgggcgcgc gcagaacagg caggtgcggc tggatatcgc
     1321 ctgggccgaa tggcagacgg ccggcgccgc gcggctgcag gcggtgcgcg tggccgcgct
     1381 ggaaacgcag gtaaccctgg cccgcagcag cgataccagc gcgcgcgcca tgcttgccag
     1441 cagcctcggg gcatcgctgc ggggcgatat cgcgggcgat gcggtgcagg ccaaccgcct
     1501 ggctgcgctg gatgccgccg aacggctgcg cgcggccgaa acggccctgg ccgccgcccg
     1561 ccaggaattg gcccgcctgc tgggcctgcc cccgggcagc cgcctgcagc tggccgacac
     1621 cgcccctgcc gtgccggccg agctggaccc ggcccggctg tttgccctgg ccgaacgcaa
     1681 ccgcacggat ctggctgccc tgcgcgccgg ctatgcggcg caggaagccg ccgttcacaa
     1741 ggccgtgctc gatcagttcc ccacgctcca gcttggcttt ggcgcctcgc gcgataccac
     1801 cggcaaccag ctgctgggct cgtcggtgaa cttcaccctg ccgctgtgga accgcaaccg
     1861 gggcggcatt gccatcgaga ccgccacccg cgcctcgctg caggccgcct atgtcgcccg
     1921 cctggcgcaa acccgcgccg acattgccgc cgccatcgac ggcatcgccc tggcccgcca
     1981 caccgccagc gacctgcaat cccagatcgc cgccctcagc gcctatgcca tgggcgccag
     2041 ccgcgcggcc gcgcatggcg atctagccgc aaccaccgcc ctgctcgccc ggcaaagcct
     2101 gctcgaccgc caatcgcagt tgagcgcggc caatcaggcg ctgggcgaac agatgatcgc
     2161 gctcgaactg ctgaccggca cccccatctc gtcctggaac cagacctcat gacccaacgc
     2221 tggatttgcg ccgccttcac cctgctcgcc gcctgctcgg ccagcgacag cgagaagacc
     2281 tccgagcctg tcgcccttgt ccagctggca gcggcccggc ccggcacgct gggcgatgcc
     2341 cttgcccttt atggcgcggt tgagccaggc gccggttcgg aacgcacgct gtccgccccg
     2401 acagaagcga tcgtcaccgc catcaccgcg ccggccggca ccggggtttc cgccggcgcg
     2461 ctggtgctcc agctgaacgc cagcccgcaa tcggcgctcg atctggccaa ggcgcgcagc
     2521 gatggcgccg ctgccgatgc cgcccttgcc cgcgccctgc gcctgaaggc ggatggcctg
     2581 gtgggcaatg ccgaggttga ggcggcgcgc gcggcggcca gcgctgccga tgccacgctg
     2641 gccagcctgc gcgcccgggc cggccagctg gccatgcgcg cgccggtggg cggcgtggtc
     2701 gatgcggtgc tggtgaagcc gggcgatctc gtcgcggccg gcgcccccct cgcgcggatc
     2761 gccaccggca gggcggtgcg cgcccgcttc gggatcgatc cggcgctgtc tgccgccatc
     2821 ccgccgggcg cgcgcatcac catcaccgcc ggcgccgccg cgctgacggt gccggtcagc
     2881 ggggttgatc gcgtggtcga tccggccacc cggctggccg ccctcttcgc caacctgccc
     2941 gccggcgcgc agatggcgct gggcgcgccg ctggccggca ccttgcagcg ccccggcgca
     3001 aagcgcggca ccctcatccc ctatgccgcc gtgcaggatg atggcggcac gcccttcgtc
     3061 tttgtggtgc agggcggcgt ggcgcatcgc cgcgaggtga gccttggcgc ccaggccagc
     3121 gggcaggtgc tggtgacggc agggctggcg ccgggcgagc aggtggtaac gcaaggcggc
     3181 accgcgctcg acgatggcat gaaggtgcgc accggcccca tcgccgatga aaaagccgac
     3241 gagaagggag cgaaggaatg aaccgcttcc tcaagcgctt cgacaaggcc atctggctgg
     3301 ccctggcgct gctgacgctg ggcgggctga tcgccgcctg gaagctgcct gtcgggctgt
     3361 ttccgcatat cgactacccg cgcatcctcg tctcgatcga tgccggcgag cgcgatccgc
     3421 aggcgatgca ggcgcagatc acccgcccgc tggaaatcgc cctgcgcgcg gtgcccggcg
     3481 tcacccagat ccgctccacc accagccgcg gcgccgccga catcgccctc agcttcgact
     3541 ggggcgagga catggttgcc gccacgctgg ccacccaggg cgcgctggcc ggcgtgcagc
     3601 ccgatctgcc ggccggcacc cgctttgccg tgcgccgctc tgatcccacc atcttcccag
     3661 tgatcggcct tgccctcacc tcgaagagcg ccgaccccaa ggccctgcgc cagttcgccg
     3721 atctcaggct gcgcccgctg ctgctcaccg tgcccggcgt tgccggcatc gacgtgctgg
     3781 gcggggccgc gcgcgaagtg caggtggaga tcgacccggg ccatctgcag gcgctggggc
     3841 tcaccatggg cgatgtcgtg caggcccttt cggcggccaa cagcgtgcgc ggcgtgggcc
     3901 gcatcgagga tcgccaccgg ctctatctgg tgctggttga cgaccggctg aaaaaccccg
     3961 gcgatgtcgg cgcgatcccc atcaaggcgg gcagcccggc ggccggcacg cgcggcaatg
     4021 ccggcgttgt cacgctgggg cagatcgccc gcatcacccc ctcctccccg ccgcaatgga
     4081 cgcgtgtgac ctcgaatggc accgatgccg tgctggtcaa catccgccag agcctgaccg
     4141 gcgatacggt ggcgatcgtc aaggcgatca acgcccggct ggccgccgaa catatccccg
     4201 cggccatcac catcgccacc ttctacgacc agtccgagct agtggcaggg gcggcgggcg
     4261 ccgtgcgcga tgccattgtg ctgggcgcgc tgctggccgg gctggtgctg ttcttcttcc
     4321 tgcgctcggg ccggctgatg ctgatcaccg ggctgatgct gccggcggtg ctggcggcca
     4381 cctgcctcgt tctggccatg ctggggcaga gcttcaacat gatgacgctg ggcgggctgg
     4441 cggccgccgt gggcctggtc gtcgatgatg cggtggtgat gctcgaacac atcatgcggc
     4501 ggatgatggg cgaggaaagc acagggccag accagctgct gaccgccgcc ggcgagatga
     4561 gccgcccgct gctgggcgct accctggcaa cggcggtggt gttcatgccg ctggccttca
     4621 tttccggggt ttccggcggc ttcttcaagg cgctggcggt caccatggtg gcggccctga
     4681 cgatctcgct cgcctatgcg cgtttcgtca tcccgctggc cacgcgccgc tggctgcgcg
     4741 caaaggatgc ccgggccgcc gaacgcgccg atggcatgat ggcctggctg gggcggcatt
     4801 atgcgcgcgc ctgccgccag gcccttgccc gcccgctgct ggttgtgggc gccacggcgc
     4861 tggtgctggg cggggccggg ctgctggcct tcagccgcgt gcccagcggc ttcatgccgg
     4921 cgatggacga gggcggcttc atcctcgact acaaggccca tcccggcgcg gccctgtccg
     4981 acaccgacca tatcctgcgc caggtcgagg cgatcatccg cgcaacgcca gaggttgcca
     5041 gctattcgcg gcgcaccggc gcccagctgg gcggcggcct cagcgaggcc gacgagggcg
     5101 atttcttcat tcgcctgaag gatggcagcc gccgcccgat cgacgatgtg atggccgaga
     5161 tccgccagcg ggtgcagcat gaggtgcccg gcatcgacat cgagaccgcg cagctgatgg
     5221 aagacctgat cggcgatctg accgccgtgc cccagccgat cgagatcaag ctgttcagcg
     5281 acaatcccga ggagctgacg ggcgcggcgc gcaaggtcgc ccgggcgatc ggccaggtga
     5341 acggcgtggt cgaggtggtg gatggcctgc gcgtggcggg cgatgccatc ctcgtcaagg
     5401 tagacagggc ggcggccgag gcgcaggggc tcgatcccga tgcggtggcc agccagctgg
     5461 aaacccagat cggcggcacc gttgccaccc agtatcagct gggcgaacag atcatcggcg
     5521 tgcgcgtgcg cggcccggcc gaccagcgcc agcgcgccga ccagctggcc gcgatgcagc
     5581 tgcgcgcgcc cgatggccat ctggtgagcg tgcggcagat cgccgcggtc accgtcaatg
     5641 ccggagagcg ccagttgacc cgcgaggatc ttgccccctt cgtggcggta acggcgcgcc
     5701 tttccgggcg cgatctgggc tcgggcatgg gcgagatcaa gggcgtgatc accgggctca
     5761 acctgccgcc ttcggtgcgg gtggaatatg gcggccttta tgcggtgcag caggccagct
     5821 tcatcgatct ggcgggcgtg ctggcggcgg ccttcctgct ggcggcgctg ctgctcacca
     5881 tcctgttcaa ccgctggcgc tacacgatcg gcgtgatggc caccgtgctg ctgtcggtgg
     5941 ccgcggtgct gctcggcctg tggatgacag gcatcgagct cgacatttcc gcgctgatgg
     6001 ggctgaccat ggtggtgggc atggtttccg aactggcgat cttctacctc ttcgagctgg
     6061 acagcgccgc tgcggcccat ggcgatgccg gcggcttgct ggaagccggc ctgggccgct
     6121 tgcggccaat cctgatgtcg gcgctgatcg ccatactgac actggcgccg ctggccctgg
     6181 gcctctcacg cggggcaggc ctgcagcagc cactggcaac ggcgatcatc ttcgggctga
     6241 cggtgggcgc gccgctggtg ctgctgttcc tgcccgccat gctgcgcatc ggcattgctg
     6301 cccgccccct gcccgatgac aggttgctgt cagcaatgac cattaagccc taatatacga
     6361 caaaaccaca agaaaggggc acccgcaccc cggggccccc gggagttgaa agatgccact
     6421 aaagcctcca tccggcgcac gcttcccctt gcgcctgcaa cggcggg
//
LOCUS       DASRYE010000182         7295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_60031,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000182 DASRYE010000000
VERSION     DASRYE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60031"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1837)
                     /locus_tag="VFF98_09510"
     CDS             complement(65..1837)
                     /locus_tag="VFF98_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU63910.1"
                     /translation="MREAPAYPILANEAARLRSLYSYDILDTPPDARFDNLAEIASSH
                     FEAPIALVSLVDQDRQWFKAAVGLGARQTPREQSFCAHAIASKDEVLIIEDAWADPRF
                     ANNPLVTGDPHIRFYAGAPILASNGDPLGTICVIDRQPRLTHPRDARFLARLAANATT
                     LLELHRNNALLHRAAERDPLTGLANRRGLDTALDAAVAAALDGRPCGLLYIDLDHFKE
                     VNDTYGHEAGDRLLCDMADRLSGCVRAYDTVARIGGDEFVILLPAQVDEAMLAQIGRR
                     ILKACEEPFIVAGTPLRLSASLGGALAPRHAVEPGELLRTADRALYAAKRDGRGQLLI
                     AGAPATEPPAEDASLEAELYRAIQENQLFLRWQAFHDLESGEVRGYEALVRWHHPRLG
                     ELSPAAFIPLAEACGLSGQLDSWVLEAATREAAAAPAHLFFAVNISAYWMARGDIARV
                     VGQVLERSGLGPHQLILEMTERIAIDNAHRAQQHMEALSGIGVAVALDDFGTGYSSLS
                     YLRQYPFDKLKLDRSFVTGIEEDPRGQVLAAGILHLAETLGIGVIAEGIETRAQAQLL
                     QAAGCRTGQGFLWGQPQAAPWLAK"
     gene            2165..2611
                     /locus_tag="VFF98_09515"
     CDS             2165..2611
                     /locus_tag="VFF98_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63911.1"
                     /translation="MTDMPLEDRIALQDLMTAYCYAVDRLDDIDTLLALFTPDATLDF
                     TRIGLPLMEGHGAIRAFFDGVFADMTHHAHHIGNFRPIRHAGDTAAMTAYVHGLGRAR
                     DGNTVDVHVEYTFDCVRTAAGWKCRRYSMFSLLPLPGSLAEIHGSR"
     gene            2651..3979
                     /locus_tag="VFF98_09520"
     CDS             2651..3979
                     /locus_tag="VFF98_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU63912.1"
                     /translation="MVDATTTYPAHPLAEQPRARGDAITGDRYWSKDFAEAEWQHMWK
                     RIWHVGGREAQLEEPGDFITHNFRHESVIMIRQKDGGIRAFYNVCRHRGNRLVQTEEG
                     GVENHLTCPYHGWKWNIDGALDWVQDEEDFSQGSPCGKLRMKEVPCETWGGFVFYSFD
                     PNAVPLKEYLNPIPELMQNRELDKWHRVVWRTLRVNTNWKFASDNFNESYHIPSVHPQ
                     FEPMIDDHYSTTVFEMYPAGHNRMIEKLQPSSRWEDAQQMKPLWAAILSEWGLNPADF
                     EGRAQDGRKALQAARRQLGPQRGYTYWNRFTDDELTDQFHHTCFPNLTMTGTPEGLHI
                     FRTEPDAEDPNWSTFDYWYLVPEIEGAREVATLYGMRPYAEAPHEAGDFTQYQAEIAQ
                     GDFLTQDLELAVTQQQGLRSMGFEDCYLADQETRVRRFHEVINDYIEGRR"
     gene            complement(4052..5521)
                     /locus_tag="VFF98_09525"
     CDS             complement(4052..5521)
                     /locus_tag="VFF98_09525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU63913.1"
                     /translation="MSILSPGFSALSPRLAPRAVPLAGTLAGWLAPALFLVLVAQSFV
                     RTGLFGWAVGIAYILYDTFLLGFTARQLWPLRRAMATPCPAPGSPRPSLGVIIAARNE
                     APVLAITIDTLAAQDHPPELILLADDGSTDDSAHVLHTLYGLERPAMGEISAPSPVLP
                     ALRWLRLPHGGKARALNAAIPLIDTEVVMTLDADTLLAPGALAAMRDAFAAEPELVAA
                     TGVLAPICGPRPMARLFQWFQSYEYVRNFLSRFAWMQQDSLLLISGAFAGFRRRAVVA
                     VGGFDPDCMVEDYELIHRLHRHAHEAGLDWRVRVIGKALASTDAPASPMAFLRQRRRW
                     FGGFLQTQHWNRDMVANPAFGKLGTRMLVVKALDTFQPVYGLVAFLILLWLAAQGRLH
                     LAGAIILVMLAKVAVDLTFHLWSIGLYKRWTGQADGLGLGQALVASLLEPFSFQLLRH
                     AGACWGWVAFLSGGGGWGRQTRTALTAGPPRAPASLPAE"
     gene            5641..6243
                     /locus_tag="VFF98_09530"
     CDS             5641..6243
                     /locus_tag="VFF98_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZU63914.1"
                     /translation="MTQSLSPARYTAVAVVLHWLVAIGIFYNLWAGLGFDDLWAAVDK
                     GALPAETARAAVELHKSIGITVLGLVVLRVLWKIGHPAPAPLAGLKPWEKKLSIGIHH
                     LLYLLIVLVPFSGWLHDSAWKAAASHPLVLFNTVPWFRLPLFGGLDDAGKDHWHAIFG
                     GIHAIGAKVLIAALLLHVAGALKHQFLDGQKLLQRMWFGR"
     gene            complement(6257..6403)
                     /locus_tag="VFF98_09535"
     CDS             complement(6257..6403)
                     /locus_tag="VFF98_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63915.1"
                     /translation="MPRQTIPARRRPALTAPPLPHPSWRGALPAQAPAAPASAREFWE
                     RLGL"
     gene            6828..7073
                     /locus_tag="VFF98_09540"
     CDS             6828..7073
                     /locus_tag="VFF98_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZU63916.1"
                     /translation="MAGYDPAGSGPILGAVNWLEGTLLGTAATVVAVMAVASVGFLML
                     TGRMNWRYGATVILGCFILFGAASIVAGIRAAAGTGY"
     gene            7082..>7295
                     /locus_tag="VFF98_09545"
     CDS             7082..>7295
                     /locus_tag="VFF98_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HZU63917.1"
                     /translation="MEPPLARDRLFVALTRPQMFAGVTYPFFVINAVVCTEGFLLLRG
                     GWVVVLAGLVHLAGMAACLEEPRRFDI"
BASE COUNT         1223 a         2330 c         2542 g         1200 t
ORIGIN      
        1 cctcccctag ggtactatcg ttgggaggcc gggtgggggc gtgggctggt gccgcccgac
       61 gccactactt tgccagccag ggcgcggcct gtggctggcc ccagaggaag ccttggccgg
      121 tgcggcagcc ggcggcttgc agcaattgcg cctgggcgcg ggtttctatg ccctctgcta
      181 tcacgccgat gcccagcgtt tctgccagat gcaggatgcc ggcggccagc acctgcccgc
      241 gcggatcctc ctctatgccg gtgacgaagc tgcggtcgag cttcagcttg tcgaagggat
      301 attggcgcag ataggacagc gaggaatagc cggtgccgaa atcgtcgagc gccacggcaa
      361 cgccaatgcc gctcagcgct tccatgtgct gctgggcgcg gtgggcattg tcgatggcga
      421 tgcgctcggt catctcgagg atcagctggt gggggccaag gccgctgcgt tccagcacct
      481 ggcccaccac ccgggcgata tcgccccgcg ccatccagta ggccgagatg ttgaccgcga
      541 agaacaggtg ggcaggggcc gcggccgcct cgcgcgttgc cgcttccagc acccagctgt
      601 cgagctggcc ggaaaggccg caggcctcgg ccagcggaat gaaggccgcc ggcgacaatt
      661 cgcccagccg cgggtggtgc cagcgcacca gcgcctcata gccgcgcacc tcgccgcttt
      721 cgagatcgtg gaaggcctgc cagcgcagga acagctggtt ttcctgaatg gcgcgataaa
      781 gctcggcttc caggctggcg tcctcggccg gcggctctgt ggcgggggcg ccggcgatca
      841 gcagctggcc gcgcccgtcg cgcttggcgg cataaagcgc gcggtcggcg gtgcgcagca
      901 gctcgcccgg ttccaccgca tggcgcgggg ccagcgcgcc gcccagcgag gccgacagcc
      961 gcagcggcgt gccggccacg atgaacggct cctcgcaggc tttgaggatg cgccggccga
     1021 tctgcgccag cattgcctca tccacctggg cgggcagcag gatgacgaac tcgtcgccgc
     1081 cgatgcgggc caccgtgtcg taggcgcgca cgcagccgct cagccggtca gccatgtcgc
     1141 acagcaggcg gtcgcccgcc tcatggccgt aggtgtcgtt gacctccttg aaatggtcga
     1201 ggtcgatata gagcaggccg cagggccgcc cgtcgagcgc ggcggcaacc gctgcatcca
     1261 gcgcggtgtc gaggccgcgg cggttggcaa ggccggtcag cgggtcgcgc tcggccgcgc
     1321 ggtgcagcag ggcgttgttg cggtgcagtt ccagcagggt ggtggcattg gccgccagcc
     1381 gggccagaaa gcgggcatcg cgcgggtggg tgaggcgcgg ctggcggtcg atcacgcaga
     1441 tggtgcccag cggatcgcca ttgctggcaa ggatgggcgc gccggcatag aagcggatat
     1501 gcggatcgcc ggtgaccagc ggattgttgg caaagcgcgg atcggcccag gcatcctcga
     1561 tgatcagcac ctcgtccttc gaggcgatgg catgggcgca gaaggattgt tcgcgcgggg
     1621 tctgccgggc gcccaggccc acggccgcct tgaaccactg acgatcctga tcaaccagcg
     1681 agaccagcgc gatgggcgcc tcgaaatggc tgctggcgat ttcggccaga ttgtcgaaac
     1741 gcgcgtcggg cggcgtgtcg agaatgtcat agctgtagag cgagcgcagc cgcgccgcct
     1801 cattggcgag aatcggatag gccggggctt cacgcatgcg actggattcg cgccgaaaag
     1861 ttaatgtggg gttaatcctt gtatcagtgg atacattttt gaaacaatca tgtttggcgc
     1921 ccgcctgtgt gccccatact agccaaatga ccagatgatc ccgcagttat ttggtgcgat
     1981 tgcaccatta tcgcggcgat caagagagaa tctaccccaa ggcagcagcc ctggggtgga
     2041 aacaggcgga gccgcgccgc cgggatagag gatgcaccgc gcgagggaag acccgcgcaa
     2101 cagccagcag gccgggcaac cggcccgcgg ctgatatcga gaaccgcttc gggaaaggcc
     2161 gtttatgacc gacatgccgc tggaagatcg cattgccctg caggatctga tgaccgccta
     2221 ttgctatgcg gtggacaggc tggacgatat cgacacgctt ctggccctgt tcacccccga
     2281 tgcgacgctg gatttcaccc gcatcggcct gccgctgatg gaaggccatg gcgccatccg
     2341 cgccttcttc gacggcgtgt tcgccgacat gacgcaccac gcccaccaca tcggcaattt
     2401 ccgccccatc cgccatgccg gcgataccgc cgcgatgacc gcctatgtgc atgggctggg
     2461 ccgcgcgcgc gatggcaata cggtggatgt gcatgtggaa tacaccttcg attgcgtgcg
     2521 caccgccgct ggctggaaat gccgccgcta ttcgatgttc tcgttgctgc cccttcccgg
     2581 cagccttgct gaaatccacg gctcgcgctg atcttcgccc ccgacagtct ggcccagaag
     2641 gagagtgcat atggttgatg ccaccaccac ctatccggcc catccgctgg ccgaacagcc
     2701 ccgcgccagg ggcgatgcga tcaccggtga tcgctattgg tcgaaggatt tcgccgaggc
     2761 cgaatggcag cacatgtgga agcgcatctg gcatgtcggc gggcgcgaag cccagctgga
     2821 agagccgggc gatttcatca cccacaattt ccgccatgaa tcggtgatca tgatccgcca
     2881 gaaggatggc ggcatccgcg ctttctacaa tgtctgccgc caccgcggca accgcctggt
     2941 gcagaccgag gaaggcggcg tggaaaacca cctgacctgc ccctatcacg gctggaagtg
     3001 gaatatcgac ggcgcgctcg actgggtgca ggacgaggag gatttctcgc agggcagccc
     3061 ctgcggcaag ctgcgcatga aggaagtgcc ctgcgagacc tggggcggct ttgtcttcta
     3121 cagcttcgat cccaatgcgg tgccgctgaa ggaatatctc aaccccatcc ccgagctgat
     3181 gcagaaccgc gagctggaca agtggcaccg ggtggtgtgg cgcaccttgc gggtgaacac
     3241 caactggaag ttcgcgtccg acaatttcaa cgagagctac cacatcccct cggtgcatcc
     3301 gcagttcgag ccgatgatcg atgatcacta ctcgacgacg gtgttcgaga tgtatccggc
     3361 cggccacaac cgcatgatcg agaagctgca gccttccagc cgctgggaag atgcccagca
     3421 gatgaagccg ctgtgggccg cgatcctgag cgaatggggc ctcaacccgg ccgatttcga
     3481 aggccgcgcc caggatggcc gcaaggccct gcaggccgcc cgccgccagc tgggcccgca
     3541 acgcggctat acctactgga accgcttcac cgatgatgag ctgaccgacc agttccacca
     3601 cacctgcttc cccaacctga cgatgaccgg cacgccggag gggctgcaca tcttccgcac
     3661 cgagcccgat gccgaagacc ccaactggtc gaccttcgac tattggtatc tggtgcccga
     3721 gatcgagggc gcgcgcgagg tggccacgct gtatggcatg cgcccctatg ccgaggcgcc
     3781 gcatgaggcg ggcgatttca cccagtatca ggccgagatc gcccagggcg atttcctgac
     3841 gcaggatctc gagctggccg tgacccagca gcagggcctg cgctcgatgg gcttcgagga
     3901 ttgctatctg gccgatcagg aaacccgcgt gcgccgcttc cacgaggtga tcaacgacta
     3961 tatcgagggc cggcgctgag gcgttgcgcc tgatttaggc aaaaggcgga tgcgggggcc
     4021 aggccttcgc atccgctttt tcgtggggcg tttattcggc cggcaggctg gccggcgcgc
     4081 gcgggggccc ggcagtgagc gcggtgcggg tttgccggcc ccagccgccc ccgcccgaaa
     4141 ggaaggccac ccagccccag caggcgccgg catggcgcag cagctggaag gagaacggct
     4201 ccagcagcga ggcgaccagc gcctggccca gccccagccc gtcggcctgc ccggtccagc
     4261 gcttgtagag gccgatcgac cacaggtgga aggtgagatc caccgccacc ttggccagca
     4321 tgacgaggat gatggcacct gccagatgca gccgcccctg cgctgccagc cacagcagga
     4381 tgagaaaggc caccagccca taaaccggct ggaacgtgtc cagcgccttc accaccagca
     4441 tgcgcgtgcc cagcttgccg aaggcggggt tggccaccat gtcgcggttc cagtgctggg
     4501 tttgcaggaa gccgccgaac cagcgccgcc gctggcgcag gaaggccatc gggctggccg
     4561 gcgcatcggt gctggccagc gccttgccga tcacccgcac gcgccagtcg agccctgcct
     4621 catgggcgtg gcggtgcagg cggtggatca gctcatagtc ttccaccatg caatccggat
     4681 cgaagccgcc cacggccacc accgcccggc gccgaaagcc ggcaaaggcg cccgagatca
     4741 gcagcaggct gtcctgctgc atccaggcga agcgcgagag gaagttgcgc acatattcat
     4801 agctctggaa ccactggaac aggcgcgcca tgggccgggg cccgcagatg ggcgccagca
     4861 cgccggtggc ggccaccagc tcaggctctg ccgcaaaggc atcgcgcatg gcagccaggg
     4921 cgcccggggc cagcagcgtg tcggcatcca gcgtcatcac cacttcggtg tcgatcagcg
     4981 gaatcgcggc attcagcgcg cgcgccttgc cgccatgggg caggcgcagc cagcgcagcg
     5041 ccggcagcac cggcgagggc gcgctgatct cgcccatggc gggccgttcc agcccataga
     5101 gcgtgtgcag cacatgggcg ctgtcgtccg tgctgccatc gtcggccagc aggatcagct
     5161 cgggcgggtg gtcctgggcg gccagcgtat cgatggtgat ggccagcacc ggcgcctcgt
     5221 tgcgcgcggc gatgatcacc ccgaggctgg gccgggggct gccgggcgcg gggcaaggcg
     5281 ttgccatggc gcggcgcagg ggccacagct ggcgcgcggt gaagcccagc aggaaggtgt
     5341 cgtagaggat ataggcgatg cccaccgccc agccgaaaag ccccgtgcgc acaaagcttt
     5401 gcgccaccag aacgaggaac agcgcgggcg ccagccagcc cgccagcgtg ccggccagcg
     5461 gcaccgcgcg cggcgccagc cgcggcgaaa gggcggaaaa tccgggcgag agaatgctca
     5521 ttgctttagc ctggccgatc tggaatgaac gcaacattgc gcgcgggccc ccttagaacc
     5581 gcaaggagag gcccgccaat ggattgtatc gaacccttgt tggtggagcc ttgctgaccc
     5641 atgacccaaa gcctctctcc tgcccgctat actgccgtgg cggtggtgct gcactggctg
     5701 gtggccatcg gcatcttcta caatctgtgg gccgggcttg gcttcgatga tctgtgggcg
     5761 gcggtggaca agggcgcgct gccggccgaa accgcccgcg cggcggtgga gctgcacaag
     5821 tccatcggca tcaccgtgct ggggctggta gtgctgcggg tgctgtggaa gatcggccat
     5881 ccggcgccgg caccgctggc ggggctgaag ccgtgggaaa agaagctgtc catcggcatc
     5941 catcacctgc tctatctgct gatcgtgctg gtgcccttct cgggctggct gcatgattcg
     6001 gcgtggaagg cggcggccag ccatccgctg gtgctgttca acaccgtgcc gtggttccgc
     6061 ctgccgctgt tcggcgggct ggatgatgcc ggcaaggacc attggcacgc cattttcggc
     6121 ggcatccatg cgattggcgc caaggtgctg atcgcggcgc tgctgctgca tgtggcgggc
     6181 gcgctgaagc atcagttcct cgacgggcag aagctgctgc agcggatgtg gttcggccgt
     6241 tgagcttgag ccgggcctaa agccccagcc gttcccagaa ttcgcgcgcg ctggcgggcg
     6301 ctgcgggcgc ctgcgccggc agcgcgccgc gccacgaggg atgcggcagg ggcggcgctg
     6361 tcagggcagg gcggcggcgg gcgggaatgg tctggcgggg catcatacaa ggctttctgt
     6421 tcggcaggcg agcccggctg ttgccccggt atcgcgcgct ttagcggttg gtgacgcgga
     6481 gcaagctgac acgagatcaa aagccgcgat cagcacggcg ggcgggccgc cgctgacgat
     6541 ttattcctac tcaactggtg tggatttgtc gcgcaaggct tgtttggggc cgggctagaa
     6601 atttttgcgc gctgggcatt ggcgccgggc cggggcaccc gcgcgcctcg ccagcgccaa
     6661 tggctgcaaa atggtggcag ttggcgttgc tgccggcccc tgcatcaccg catgaatacc
     6721 taggtaatca ggaggggttc atgcggcggg aagcagggtt ttgcaaatgg ctgtatcggg
     6781 ccggcgggct ggccgggctg ggctgggcag gcggtgcgcg ggcgcagatg gcgggctatg
     6841 atccggccgg atcaggcccg attctcggcg cggtgaactg gttggaaggc acgctgctgg
     6901 gcaccgcggc cacggtggtg gcggtgatgg cggtggcctc ggtgggcttc ctcatgctca
     6961 ccgggcggat gaactggcgc tatggcgcca cggtgatcct gggctgtttc atcctgtttg
     7021 gcgcggccag catcgtggcg ggcatccgcg cggcggctgg caccggctat taggcgcggc
     7081 gatggaaccg cccctggcga gagaccggct gttcgtggcc ctcacccggc cgcagatgtt
     7141 tgccggcgtt acctatccgt ttttcgtcat caatgcggtg gtgtgcaccg aaggatttct
     7201 gctgctgcgg gggggctggg tggtggtgct ggcggggctg gtgcatctgg ccggcatggc
     7261 cgcctgcctg gaagagccgc gccggttcga tatct
//
LOCUS       DASRYE010000183         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_81801,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000183 DASRYE010000000
VERSION     DASRYE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81801"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFF98_09550"
     CDS             <1..655
                     /locus_tag="VFF98_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63918.1"
                     /translation="AGAKGARLVLVGRDGARAAEVAATLQASGLEALGHGLDARDGEA
                     LNALLDTLGPIDHAISMLGGAMGGGFLSADPAQIRSTIEGKLFDNLALARVLTPRLQE
                     GGSLTVTGGSGGRPDNASGAYIGNIGIAALVRGLAVELAPGIRVNAVAPTWTPTGLWR
                     DVARSDVEATAAQFAGLIPLGRVAQVEEVAMAYLFLMECTFITGQTLTVDGGLTLIH"
     gene            complement(730..805)
                     /locus_tag="VFF98_09555"
     tRNA            complement(730..805)
                     /locus_tag="VFF98_09555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(770..772),aa:Val,seq:tac)
     gene            complement(914..1786)
                     /gene="purU"
                     /locus_tag="VFF98_09560"
     CDS             complement(914..1786)
                     /gene="purU"
                     /locus_tag="VFF98_09560"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZU63919.1"
                     /translation="MPEGRVLTDYLLTLSCRNRPGIVAAISAVILAHAGDITEASQFD
                     DAGAGRFFMRLVFAVPVDADAAALRRDLAGVAQRFAMDWALRALALRQKVLLLVSRFD
                     HCLVDLLYRLRMGELPMEVVGVVSNHPRAALGDLALGAIPFHHLPVSPADRPAQEAAI
                     KRIVAQTGADLVVLARYMQILSDDLASFLAGRCINIHHSFLPGFKGAKPYHQAHARGV
                     KLIGATAHYVTRDLDEGPIIEQDVERISHADRPEDLVRKGRDIERRVLARALRLHLEG
                     RCLMHGQKTIVFRD"
     gene            complement(1779..2216)
                     /locus_tag="VFF98_09565"
     CDS             complement(1779..2216)
                     /locus_tag="VFF98_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HZU63920.1"
                     /translation="MAEAGVEEITGFALAMVLGGDGGEGVGGAAWLPLGPGRALAFAP
                     SGSAGLAALAGPEAIVVDQSSAWRLFALSGAGGWRVLQAGIPVDLSPAAFAPGAVVAS
                     LIGHIGIIAHHAAPGRYHLFVARSLADSLRHWLEATAASLPDA"
     gene            complement(2209..>3792)
                     /locus_tag="VFF98_09570"
     CDS             complement(2209..>3792)
                     /locus_tag="VFF98_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZU63921.1"
                     /translation="ARQAAEALGRSATLPPLPPLPQWPAADEGVPAIHPVWASGPCRA
                     KAFVDFQHDVTARDVELAVQEGFTSVEHLKRYTTLGMATDQGRVGQVNGHGLLAAATG
                     RSLAQTGTIRPRPPLVPVAIGAFAGGHRGVHFRPTRLAPSHGWAAARGAVFVDAGPWK
                     RAAWYPLPGEHHWRQSVDREVGAVRQGVGLCDVSTLGKIELFGADAGTLLDRLYINRL
                     SGLAVGKARYGLMLREDGMALDDGTVTRLAADHWFITTTTGNAAQVMQHLEFARQVLW
                     PDLDAQAVSVTEQWATFSLAGPRSREVLAAALPQVDVADAALPHMGVRQARWQGDALR
                     LFRLSFSGELAYEIAVPAARAEALLAHLFAKGAGFGLVPYGLEALAVLRIEKGHPAGN
                     ELNGWTTAADLGLGRMASTRKDHIGRLMAARPALADPARPALVGLRPADGTSPLRAGA
                     HLLPCGAPAVAAHDEGYVSSACFSPTLGHPIALGFLARGRARIGEEVIVHDPVRGGDV
                     RAWVCDPAFYDPAGEKLRG"
BASE COUNT          633 a         1366 c         1291 g          502 t
ORIGIN      
        1 ggcgggggcg aaaggcgccc gcctggtgct ggtcgggcgc gatggcgcca gggcggccga
       61 agtcgccgca accttgcagg caagcggtct cgaggcgctg gggcatgggc tcgatgcgcg
      121 tgatggcgaa gcgctgaacg ccttgctcga tacgctgggt cccatcgacc acgcgatctc
      181 gatgctgggc ggggccatgg ggggcggatt tctctcggcc gatccggcgc agatccgctc
      241 caccatcgag ggcaagctgt tcgacaatct ggcgctggcc cgcgtgctga cgccgcgcct
      301 gcaagagggc ggcagcctga ccgtgaccgg cggctctggc gggcggcccg acaatgcctc
      361 gggcgcctat attggcaaca tcggcattgc cgcgctggtg cgcgggctgg cggtggagct
      421 ggcgccgggc attcgcgtca atgcggtggc gccgacatgg accccgaccg gcctgtggcg
      481 tgacgttgcg cgctccgatg tcgaggcgac cgctgcgcag ttcgccgggc tgatcccgct
      541 tggccgggtc gcgcaggtcg aggaggtggc catggcctat ctgttcctga tggaatgcac
      601 cttcatcacc ggccagacgc tgaccgtgga tggcgggctg acattgatcc actgaccgac
      661 cgcgccacgg gagccagaga accctggcct tcctgaagcg atgccggtgg gcgttggcga
      721 tcaagaaaat ggtgggcggt gacgggctcg aaccgccgac cctctcggtg taaacgagat
      781 gctctaccaa ctgagctaac cgcccgctgc gatggcgcag atggcctgcg cgctttcagc
      841 gaagcgccgg tctctacacg agccgccctc ccggtcaaca gccatgttgg ccgggcgccg
      901 ccgcagcccc gcgctagtcg cgaaagacga tggtcttctg gccatgcatc aggcagcgcc
      961 cttccagatg caggcgcagg gcgcgggcca gcacccggcg ctctatgtcg cggcccttgc
     1021 gcaccaggtc ctcgggccgg tcggcatggc tgatgcgctc gacatcctgc tcgatgatcg
     1081 gcccctcatc cagatcgcgc gtcacgtaat gggcggtggc gccgatcagc ttcacgccgc
     1141 gcgcatgggc ctgatggtag ggcttggcgc ccttgaaacc gggcaggaag gaatggtgga
     1201 tgttgatgca gcggccggcc agaaagctgg ccagatcatc cgagaggatc tgcatatagc
     1261 gcgccagcac caccagatcg gccccggttt gcgccacgat ccgcttgatc gcggcttcct
     1321 gcgcgggccg gtcggcgggg ctgaccggca ggtgatggaa gggaatggcg cccagcgcca
     1381 gatcccccag cgccgcgcgc gggtggttgg agacaacgcc caccacttcc atcggcaatt
     1441 cccccatccg cagccggtag agcagatcga ccagacagtg atcgaaccgg ctgaccagca
     1501 acagcacctt ttgccgcaag gccagcgccc gcagcgccca atccatggcg aagcgctgcg
     1561 ccacaccggc cagatcgcgc cgcagcgccg ccgcatcggc atcaactggc accgcgaaca
     1621 ccagccgcat gaaaaagcgc cccgccccgg catcgtcgaa ctgcgaggct tcggtgatat
     1681 cgccggcatg ggccagaatg actgccgaaa tggccgccac aatgccgggc cggttgcggc
     1741 aggaaagggt gagcagatag tcagtcaaga cgcgtccttc aggcatcggg caggctggcg
     1801 gcggtggcct ccagccagtg gcgcaggctg tcggccaggc tgcgcgcgac aaacaggtga
     1861 tagcggccgg gcgctgcatg gtgggcgatg atgccgatat ggccgataag gctggccacc
     1921 accgccccgg gcgcaaaggc cgccggcgac agatcgaccg gaatgcccgc ctgcagcacg
     1981 cgccagcccc ccgcgcccga cagggcaaac aggcgccagg cgctgctctg gtcgaccacg
     2041 atggcctctg ggccggccag cgcggcaagg cccgcggagc ccgatggtgc aaaggccagc
     2101 gcccgccccg ggcccagcgg cagccatgcg gccccgccaa cgccctcgcc cccatccccg
     2161 cccagcacca tcgccagcgc aaagccggtg atttcctcca cacccgcctc agccacgcag
     2221 tttctcccct gccggatcat aaaacgccgg atcacacacc cacgcccgca catccccccc
     2281 gcgcaccgga tcatgcacga tcacctcctc gccgatccgc gcccggccac gcgccagaaa
     2341 gcccagcgca atcgggtggc ccagcgtggg cgagaaacag gcggaagaga catagccctc
     2401 gtcatgcgcg gcaacggccg gcgcaccgca gggcagcaga tgcgcgccgg cccgcagcgg
     2461 cgaagtgcca tctgcagggc gcagccccac cagcgccggg cgggcagggt ctgccagcgc
     2521 cgggcgggcg gccatcagcc ggccgatatg gtccttgcgg gtggaggcca tgcgccccag
     2581 gccgagatcg gctgccgtcg tccagccgtt cagctcgttg ccggccgggt ggcccttttc
     2641 gatgcgcagc accgccagcg cctccagccc atagggcacc aggccaaaac cggcgccctt
     2701 ggcgaaaaga tgggccagca gcgcctcggc ccgggccgcc ggcacggcga tctcgtaagc
     2761 cagctcgccc gagaaggaga ggcggaagag gcgcaaggca tcgccctgcc agcgcgcctg
     2821 ccgtacgccc atatgcggca gggcggcatc ggccacatcc acctgcggca gggccgcggc
     2881 cagcacctcg cgcgatcgcg ggcccgccag cgagaaggtg gcccattgct cggttaccga
     2941 aacggcctgc gcatccagat ccggccacag cacctgccgg gcaaattcca gatgctgcat
     3001 cacctgtgcc gcattgccgg tggtggtggt gatgaaccag tggtcggcgg ccagccgggt
     3061 cacggtgcca tcatccagcg ccatgccatc ctcgcgcagc atcagcccat agcgcgcctt
     3121 gcccaccgcc aggccagaca ggcggttgat gtagagccgg tcgagcaaag tgccggcatc
     3181 ggcgccgaac agctcgatct tgcccagcgt ggagacatcg cacagcccca ccccctggcg
     3241 cacggcgcca acctcgcggt caaccgactg gcgccagtgg tgctcgcccg gcagcggata
     3301 ccaggccgcc cgcttccacg gcccggcatc gacaaacacc gccccgcgcg cggctgccca
     3361 gccatgcgag ggcgccagcc gcgtgggccg gaaatgcacg cccctgtgcc ccccggcaaa
     3421 ggcgccgatg gccaccggca ccagcggcgg gcgcgggcgg atggtgcccg tctgcgccag
     3481 gctgcggccg gtggcggcgg ccagcaggcc atgcccgttc acctgcccca cccggccctg
     3541 atcggtggcc atgcccagcg tggtgtagcg cttcagatgc tcgacgctgg tgaagccttc
     3601 ctgcacggcc agttccacat cgcgggcggt cacatcatgc tggaaatcga cgaaagcctt
     3661 ggcccggcac ggcccgctgg cccacaccgg gtggatggca gggacgccct catccgcggc
     3721 cggccattgc ggcaggggcg gcaggggcgg cagagtggcg ctgcggccca gcgcttctgc
     3781 cgcctgccgc gc
//
LOCUS       DASRYE010000184        14450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_10819,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000184 DASRYE010000000
VERSION     DASRYE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10819"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFF98_09575"
     CDS             complement(<1..445)
                     /locus_tag="VFF98_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63922.1"
                     /translation="MLRQNTLHYGMDPDPHGGPDSGAPGRQAVVVLGMHRSGTSALAR
                     IVSLLGADLPATLMPADASNVAGHWESGPVCGVNEALLASAGSRWDDWQGLNPAWYGS
                     AREAQFAAQASAVLAAEYGASGLFVLKDPRICRLYPFWRAVLGRAG"
     gene            complement(607..1023)
                     /locus_tag="VFF98_09580"
     CDS             complement(607..1023)
                     /locus_tag="VFF98_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HZU63923.1"
                     /translation="MDWMLLPYRRYFDFSGRSRRREFWLFSLFCMCVNLAIDFGLGGP
                     YATLTGYSAMVGTHPGFLAQGLTGLFGLFSFIPSWAVAVRRLHDIDRSGWWLLLVLLP
                     VLGWFVLLVFNCLDGTAGRNSYGFDPKGRGFDEVFR"
     gene            complement(1147..3753)
                     /locus_tag="VFF98_09585"
     CDS             complement(1147..3753)
                     /locus_tag="VFF98_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63924.1"
                     /translation="MRQGGMGRALGAGPGEWRQVMRALALGGAGAGALALAGMARAAD
                     ADTGADTGADAAHPPAAPVVEEGNAILVTATKREQTLQDVPVAVTVTTGATLHDAQIR
                     DLLDLTSVVPSLQVVQEQSSTQMYFSIRGFGNGANNAGIEPSVGVFVDGVYRSRSVSQ
                     IADFPDVARVEVLRGPQSTLFGKNASAGVISIVTDLPKFTLGGSAELSYGNYNALVAK
                     ATLTGPITDTLAFSLAGGFNRRDGYQHDAATGADINNRNRGFVRGQLLYDPASGPRVR
                     LIADWGQIDELCCAVTNLRASSTTAAIMALGGQVNAAANPYADTVYNNFNSTNKLTNY
                     GVSGQVDYQWGQVKLTGITAWRHSHADQNQDSDFTSLDILGENALDQSIDTFTQELRA
                     TTTLGKAVNLLFGAYYFNEHIRQTGELRYGTQMRPYADLLIQGATGGAENLAQVEQFV
                     GAYGAGNPAAYVGQFFTPGSGLSEAYRLANESYSLFAQADIHLTRRLTLTGGINYTHD
                     GKRFSTHVTSSDVFSGIDLAQVVGNALTAGVPAASAYPLLALRALQFMPPFLNLPNAA
                     EPGRTNDNNVSFTARLAYDVSRHVNAYVSYATGFKASSVNLSRDSRPALADAAAIVGD
                     GLGLANLRYGSRFAGPERSKVAEVGLKGNWGRASLNLAGFYEEIDGFQSNTFTGIGFD
                     LENAEKESVWGLEVEGQARVARELVLSGSLTYLAPKYDRYTNSSFGDVSGATPAGIPP
                     VSLTIAATEDHPLANGDHIVLRADWHYEAPYQVEDGLPGFITTNPLTGQVISYQSGID
                     AARPYRALVSEVDGSLAYRRHGGVELSVWTRNMFNHRTLIAVFDSPAQQGSVSGYTNQ
                     PRTFGVAAGWKF"
     gene            4068..5909
                     /gene="typA"
                     /locus_tag="VFF98_09590"
     CDS             4068..5909
                     /gene="typA"
                     /locus_tag="VFF98_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZU63925.1"
                     /translation="MSAPLPLRNIAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERA
                     MDSNDLEKERGITILAKPTSIEWTGHESGPVRINIVDTPGHADFGGEVERILSMVDGV
                     ILLVDSSEGAMPQTKFVTGKALALGLRPIVVVNKVDRPDQRIQEVLDEVFDLFVSLDA
                     DDEQLDFPVLYASGRNGYAGYDQDAREGTLEPMFKLIVDHVPAPNLDVDAPFTFLATL
                     LDRDNFMGRVLTGRVQSGVIKVNDPIRALDAEGNVIETGRASKLMAFRGLERVPVDEA
                     KAGDIISLAGLTVATVANTIAAPEVVEPIKAQPIDPPTLSMRFAVNDSPLAGREGSKV
                     TSRMIRDRLEREAESNVAIKITESADKDSFEVAGRGELQLGVLIETMRREGFELGISR
                     PRVLFGTDENGQRTEPYETVLIDVDEEFSGTVVEKMALRKGEMTDMRPSGGGKTRITF
                     IAPSRGLIGYHGEFLSDTRGTGIMNRLFDKYGPYKGKIEGRPNGVLISNGDGEAVAYA
                     LNSLEDRGVLFVSPQEKIYEGMIIGENAKPDDLEVNPMKSKQLTNFRSTGKDDAIRLT
                     PPRIMTLEQAIAYIDDDEMVEVTPKSVRIRKAILDPNERKKASRKAD"
     gene            6158..6841
                     /locus_tag="VFF98_09595"
     CDS             6158..6841
                     /locus_tag="VFF98_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63926.1"
                     /translation="MHRFATLGLSTTGLSTTGLTTTSLATIGLAAALLGLGGCTLPPA
                     DSSGSAASAAAADGSSESSVSLDALLNRGTNVLTIKNTSGGALVLQRVVVNGQEGADS
                     CNNKLFVDMANAASRDVSLGDCGVVRSTKVYTDHGTYSSDWTYEPFIDLSVSSGSITV
                     KNLGINKYVVQSIILNHRKNDSNCDIKVFRDLDASGSLDVYGTDQCGEIKHYEVVTDK
                     GTREFGDDL"
     gene            7025..7516
                     /locus_tag="VFF98_09600"
     CDS             7025..7516
                     /locus_tag="VFF98_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63927.1"
                     /translation="MIQFLKKGLLASAIAASAVASFAATPAMADPWRGGYRGGYHGGD
                     AAGAAVAGGIIGLAVGAAIASDRPHYVAPPPYAPAYVAPPAYYYDEPAYYVDPAWVWR
                     DGWYWDRMGHRYYRDGRPWGYAAGWRGGWHDGGHWGGPGWRGGEHWGGGHWGGEHGGW
                     HGR"
     gene            7661..8818
                     /locus_tag="VFF98_09605"
     CDS             7661..8818
                     /locus_tag="VFF98_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZU63928.1"
                     /translation="MTIPAKTDELRVALFSGNYNYVRDGANQSLNRLVGYLLRQGAKV
                     RVYSPTIETPAFPPTGDLVSLPSLPIPGRAEYHLATGLSAAVRRDLEGFAPNIVHISS
                     PDPSAHRAVTWARKRGIPVLASVHTRFDTYPRYYNLGWIEPALTAILRRLYRRCDALV
                     VPSESMAQVLRDQRMNYDISIWSRGVDREIFTPARRDMVWRQAQGFADNEVVIGFFSR
                     LVMEKGLDVFADAIDELKARGVPHRLLVVGDGPARDWFENRLPGAVFTGFQGGTDLAR
                     AVASMDLLFFPSVTETFGNVTLEAMACGLPVVAAAATGSESLVDDHVSGRLIRPGAIH
                     AYAEALRAYVESADLRAAHGAAGLARAQEFSWDRINQSVADTYLRLIRQRG"
     gene            complement(8843..9268)
                     /locus_tag="VFF98_09610"
     CDS             complement(8843..9268)
                     /locus_tag="VFF98_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63929.1"
                     /translation="MSRCPDQEMGRFRLAVWACLVWNLFNVGRFWMLEMHLAPGFLQP
                     VVEWLDGLPLYMLILREASVYAPLAGAGLMLLRSRHAAVAFVIGFAASAACYSQIMLH
                     HFGDPRCTLLVQWVLAVRQIAIGVQWWLARRMFWAGYLD"
     gene            9401..10747
                     /locus_tag="VFF98_09615"
     CDS             9401..10747
                     /locus_tag="VFF98_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZU63930.1"
                     /translation="MADLFASAADSAPAAPALPGAGTGAAMPLAERLRPASLAEVVGQ
                     EHLTGPEGAIGRMVTAGRLSSMILWGPPGTGKTSIARLLAAAVGMRYAQISAVFSGVA
                     DLKKAFAEAEGALSLQGRRTLLFVDEIHRFNRAQQDGFLPYVEAGTVTLVGATTENPS
                     FALNAALLSRAQVLILHRLPADALTRLMQRAEEAEGVPLPLTPPAREALVASADGDGR
                     FLLGQIETLYGASLFAPLDPAGLGQFLQRRVAVYDKDRDGHYNLISALHKSLRGSDPQ
                     AALYYLARMLTAGEDPLYVLRRLVRFASEDVGLADPAALPQCLAARDAYQFLGSPEGE
                     LAIAQACLYLATAPKSNAAYAAWKEAAATAKATGSLMPPANILNAPTKLMKEIGYGQN
                     YAYDHDAEDGFSGANYWPEGMAPQSFYRPVERGFEREVLKRLDWWEKKRRERQQGG"
     gene            10756..11652
                     /locus_tag="VFF98_09620"
     CDS             10756..11652
                     /locus_tag="VFF98_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63931.1"
                     /translation="MPPRFTHFAAIDWSGAAGTSHKGIAVAICAAGDAAPVLVAPPSP
                     RGWARTEVLRWLVESAPPTTMIGFDMGISLAHADCGAFFPGWAQSPADARALWALVEE
                     ICAGEPDLGVAPFLAHPQAARHFRQQGGRLGDLFPTSGPTGARGRWRVAEEAQAAMGC
                     APTSNFNLVGAAQVGKASLAGMRLLHRLPDSIAVWPVDEVAPRRKVVAEIYTAIAAIA
                     AGRRAGASKIRSHEALAEALAHPAIASAAPTGTGPIADHAADALLTAAWLRRHAHLPA
                     LWAPARLTPALAHTEGWTFGVA"
     gene            11670..11744
                     /locus_tag="VFF98_09625"
     tRNA            11670..11744
                     /locus_tag="VFF98_09625"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11702..11704,aa:Thr,seq:tgt)
     gene            complement(11855..11930)
                     /locus_tag="VFF98_09630"
     tRNA            complement(11855..11930)
                     /locus_tag="VFF98_09630"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11895..11897),aa:Trp,seq:cca)
     gene            12033..12866
                     /gene="aat"
                     /locus_tag="VFF98_09635"
     CDS             12033..12866
                     /gene="aat"
                     /locus_tag="VFF98_09635"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZU63932.1"
                     /translation="MLAPVSNRPAHALIDLPLLMLAYRSGIFPMADARDDPEVFWVEP
                     KRRGILPLDGFHCARSLARVLKRGTFTVTCDEAFADVLAACAAPRAESPDTWINQRIA
                     ATYIALHAAGHAHSLECWQTGADGQRRLVGGLYGVGFDRVFCGESMFSRVSDASKVAL
                     AWLVAALRAGGYRLLDCQFLTDHLGSLGAVEISQKRYLELLRAAQSPESPHAGVDQRG
                     ASGVGAASGAAGTGAGSADAAEEALALPEAFGALLAAAKSAGFSSSPGNFIAQSFTQT
                     S"
     gene            complement(12902..13321)
                     /locus_tag="VFF98_09640"
                     /pseudo
     CDS             complement(12902..13321)
                     /locus_tag="VFF98_09640"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(13330..13734)
                     /locus_tag="VFF98_09645"
     CDS             complement(13330..13734)
                     /locus_tag="VFF98_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HZU63933.1"
                     /translation="MLFTWWNGATFGTWLNTRRNGELVGTDYQGNTYYRSRKKIPAGA
                     NSAFAGRERRWVIYKGNNDASRVPAEWHGWLHGTYDGVPESHLPPPRIWEVEYTPNAT
                     GTVAAYRPAGALEKGGKRAAATGDYEAWTPEG"
     gene            complement(13903..>14450)
                     /locus_tag="VFF98_09650"
     CDS             complement(13903..>14450)
                     /locus_tag="VFF98_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZU63934.1"
                     /translation="RLDTYLAGKVRRAGWQAAGQGEPLAGEAALAAAQEVIAALVARA
                     VAAGYVDDAGFARARSASLLRRGYGARRVDQALDAAGIDEEIRAGLRPDESAARAAAL
                     AFARRRGLGPFTREGQKEDPRRREKQIAALLRAGHGFGVARALVDSPDVAAAQGWAAG
                     DGAADEGDGPADDGPAADLWE"
BASE COUNT         2426 a         5066 c         4633 g         2325 t
ORIGIN      
        1 cgcccgcccg ccccagcacc gcccgccaga acggatacag ccggcaaatg cgcggatcct
       61 tcagcacgaa caggcccgag gcgccatatt ccgccgccag caccgccgat gcctgggcgg
      121 caaactgcgc ctcgcgcgcg ctgccatacc aggccgggtt caacccctgc caatcatccc
      181 agcggctgcc ggcagaggcc agcagcgcct cgttcacccc gcacaccggg cccgattccc
      241 aatggccggc cacattgctg gcatcggccg gcatcagcgt ggcgggcaga tccgccccca
      301 gcaggctgac gatgcgcgcc agcgcgctgg tgccggaacg gtgcatgccc agcaccacca
      361 ccgcctgccg gcccggcgcg ccgctatcgg gcccgccatg cggatcagga tccatcccgt
      421 aatgaagtgt gttctggcgg agcatgaggg ccagcctccc gcatctggcc ctctaggtat
      481 tatccccgca gcgccgccgc cgcaggcccg gatacatccg ttttccagca agcctgcccc
      541 cggccaaccc gccccgccgc agattgccgg gcttttgccc ccttcattgc ggagattttc
      601 ccgcccctag cggaacacct cgtcaaaccc gcggcccttg ggatcgaagc cataggaatt
      661 gcgcccggcc gtgccatcga ggcaattgaa caccagcagc acgaaccagc ccagcaccgg
      721 cagcagcacc agcagcagcc accagcccga ccggtcgata tcatgcaggc ggcgcaccgc
      781 caccgcccag ctgggaatga agctgaacag gccgaacagc cccgtcagcc cctgcgccag
      841 aaagcccgga tgcgtaccca ccatcgccga atagccggtg agcgtggcat agggcccgcc
      901 caggccaaaa tcgatcgcca gattgacgca catgcaaaac aggctgaaca gccagaattc
      961 ccgccggcgc gagcggccgg aaaaatcgaa atagcgccga tacggcagca gcatccagtc
     1021 catcgccccg attccccttt cgtgcccgcg cctggcaggg caaccccagc gcaccacaaa
     1081 aagcgccccg aggcaatggt ttgccagagg aaaggggggc ttggcaaagg aaagcggggc
     1141 ccctcgtcag aacttccagc cggcggccac gccaaaggtg cgcggctggt tggtgtagcc
     1201 cgagacagag ccctgctgcg cgggcgaatc gaacacggcg atcagcgtgc ggtggttgaa
     1261 catgttgcgc gtccacacgc tcagctccac gcccccgtgc cggcgatagg ccagcgagcc
     1321 atccacctcg ctcaccagcg cgcgataggg gcgggcggca tcgatcccgc tctgatagga
     1381 gatcacctgc ccggtcagcg ggttggtggt gatgaagccg ggcaggccat cctccacctg
     1441 atagggcgct tcataatgcc agtctgcccg cagcacgata tggtcgccat tggccagcgg
     1501 atggtcttcc gtcgccgcga tcgtcaggct gacgggggga atgcccgccg gcgtcgcgcc
     1561 cgagacatcg ccgaaggagg aattggtgta gcggtcgtat ttcggcgcca gataggtcag
     1621 cgatccgctc agcaccagct cgcgcgccac gcgggcctgg ccttccactt ccagccccca
     1681 caccgattcc ttctcggcgt tttccagatc aaagccgatg ccggtgaagg tgttcgactg
     1741 gaacccgtcg atctcctcgt agaaaccggc aaggttcagg ctcgcccggc cccaattgcc
     1801 cttcaggccc acttcggcca ctttcgaccg ctccggcccg gcaaagcggc tgccatagcg
     1861 cagattggca aggccaaggc catcgcccac gatcgccgcc gcatcggcca gcgccggccg
     1921 gctgtcgcgc gagaggttga cgctgctggc cttgaagccg gtggcatagc tgacataggc
     1981 attcacatgc cggctgacat cataggccag ccgcgcggtg aagctgacgt tattgtcatt
     2041 cgtgcggccc ggctctgccg cattgggcag gttgaggaag ggcggcatga attgcagcgc
     2101 ccgcagggcc agcagcggat aggcgctggc cgccggcacg cctgcggtaa gggcattgcc
     2161 caccacctgc gccagatcga tgcccgagaa cacatcgctg gaggtgacat gggtggaaaa
     2221 gcgcttgcca tcatgggtgt agttgatgcc gccggtcagc gtcagccggc gggtcaggtg
     2281 gatatcggcc tgggcaaaca gcgaatagct ctcgttggcg aggcgatagg cttccgacag
     2341 gcccgagccg ggggtgaaga actgccccac ataggccgcc ggattgcccg ccccataggc
     2401 ccccacgaac tgctccacct gcgccaggtt ttccgcgccg ccggtggcgc cctggatcag
     2461 cagatcggca taggggcgca tctgcgtgcc atagcgcagc tcgccggtct ggcggatatg
     2521 ctcgttgaag taataggcac cgaacagcag gttcaccgcc ttgcccagcg tggtggtggc
     2581 gcgcaattcc tgcgtgaacg tgtcgatcga ctggtcgagc gcgttctcgc ccagaatgtc
     2641 gaggctggtg aagtctgaat cctggttctg gtcggcatgg ctgtggcgcc aggcggtgat
     2701 gccggtcagc ttcacctgcc cccactgata atccacctgc cccgaaacgc cataattggt
     2761 gagcttgttg gtggaattga aattgttgta caccgtgtcg gcatagggat tggcggcggc
     2821 attcacctgc ccgcccagcg ccatgatcgc cgccgtggtg gacgaggcgc gcaggttcgt
     2881 caccgcgcag cacaattcat cgatctgccc ccaatcggca atcaggcgga cgcgcgggcc
     2941 actggccgga tcatacagca actgcccgcg cacaaagccg cggttgcggt tgttgatatc
     3001 ggcgccggtg gcggcatcat gctgatagcc atcgcgccgg ttgaaaccgc cggccaggct
     3061 gaaggccagc gtgtcggtga tcggccctgt caacgtggcc ttggccacca gcgcgttgta
     3121 attgccatag gaaagctctg ccgagccgcc cagcgtgaat ttcggcagat cggtgacgat
     3181 ggagatgacg ccggcgctgg cattcttgcc gaacagggtg gattgcggcc cgcgcagcac
     3241 ttccacccgc gccacatcgg gaaaatcggc gatctgcgaa acgctgcgcg agcggtagac
     3301 gccatcgacg aagaccccca ccgagggttc gatcccggca ttgttggcgc cattgccaaa
     3361 gccgcggatg gaaaaataca tctgggtgga gctttgctcc tgcaccacct gcagcgaggg
     3421 gacgacgctg gtgagatcga gcagatcgcg gatctgggca tcatgcaggg tggcgccggt
     3481 ggtcaccgtc acggccacgg gcacatcctg cagggtctgc tcgcgcttgg tggcggtaac
     3541 gaggatggca ttgccttcct ccaccacagg cgctgccggc gggtgggcgg catcggcccc
     3601 ggtatcggcc ccggtatcgg catcggccgc ccgggccatg cctgccagcg ccagcgcgcc
     3661 cgcccccgcg ccgcccagcg ccagcgcccg catcacctgc cgccattcgc ccggcccggc
     3721 gcccagcgcc cggcccatgc ccccctgtcg catcctcgtc ccccaaaacc tctcgcaccg
     3781 gccggccccc gccaagctgg gccgcacccg attcttctgg cacgggcccg gcctttgcgc
     3841 cggtttcacc ctgccataat gatgtttagg cacagtaaac cttgcggcac aatcgcaatc
     3901 tgcaccgctt tgcgacacac cctgcccccc tgcggcattt ttccccgatg cccgcgcgct
     3961 tgcccaaagg ccgcccttgg gctagggcgc gcggcaattg tgcggtgcgc catgccccgc
     4021 gccccgttcc gcatgcccgc cgaccgggcc cagtcgaaag ttgcttcatg tccgcgcccc
     4081 ttccgctgcg aaatatcgcc atcatcgccc acgttgacca cggcaagacc acgctggttg
     4141 accagctgtt ccgccagtcg ggcactttcc gcgacaacca gcgcgtggaa gagcgcgcga
     4201 tggacagcaa cgatcttgaa aaagagcgcg gcatcaccat cctggccaag cccacctcga
     4261 tcgagtggac cggccatgaa tcgggccccg tgcgcatcaa catcgtcgac acccccggcc
     4321 acgccgactt cggcggcgag gtggagcgca tcctctcgat ggtcgacggc gtgatcctgc
     4381 tggtggactc gtcggaaggc gccatgccgc agaccaagtt cgtcaccggc aaggcgctgg
     4441 cgctgggcct gcgccccatc gtcgtggtga acaaggtgga ccgccccgac cagcgcatcc
     4501 aggaagtgct ggacgaagtg ttcgacctgt tcgtcagcct cgacgccgac gatgaacagc
     4561 ttgatttccc cgtactttat gcttcgggcc gcaatggcta cgccggctat gaccaggatg
     4621 cccgcgaagg cacgctggag ccgatgttca agctgatcgt cgaccatgtg ccggccccca
     4681 acctcgatgt ggacgccccc ttcaccttcc ttgccacgct gctggatcgt gacaacttca
     4741 tgggccgcgt gctgacgggc cgcgtccagt cgggcgtcat caaggtcaac gatcccatcc
     4801 gcgcgctcga tgccgagggc aatgtgatcg agaccggccg cgcctccaag ctgatggcct
     4861 tccgcggcct cgagcgcgtg ccggtggacg aggccaaggc gggcgacatc atcagccttg
     4921 ccggccttac cgtggccacc gtggccaaca ccattgccgc gcccgaagtg gtggagccga
     4981 tcaaggccca gccgatcgac ccgccgaccc tctcgatgcg ctttgcggtg aacgattcgc
     5041 cgctggccgg ccgcgaaggc agcaaggtga ccagccgcat gatccgcgac cgcctggagc
     5101 gcgaagcgga atcgaacgtt gccatcaaga tcaccgaatc ggccgacaag gacagcttcg
     5161 aggtggccgg ccgcggcgaa ctgcagctgg gcgtgctgat cgagacgatg cgccgcgaag
     5221 gcttcgagct gggcatcagc cgcccgcgcg tgctgttcgg caccgacgag aacggccagc
     5281 gcaccgagcc ttacgagacc gtcctcatcg acgtggacga ggaattctcg ggcacggttg
     5341 tggaaaagat ggccctgcgc aagggcgaga tgaccgacat gcgcccctcg ggcggcggca
     5401 agacccgcat caccttcatc gccccctcgc gcggcctcat cggctatcac ggcgagttcc
     5461 tttcggacac gcgcggcacc ggcatcatga accgcctgtt cgacaagtat ggcccctaca
     5521 agggcaagat cgaggggcgg cccaatggcg tgctcatctc gaacggcgac ggtgaggcgg
     5581 tggcctacgc gctcaacagc ctggaagacc gcggcgtgct cttcgtctcc ccgcaggaga
     5641 agatctatga gggcatgatc atcggcgaga acgccaagcc cgacgatctt gaagtgaacc
     5701 cgatgaagtc gaagcagctg acgaacttcc gttcgaccgg caaggacgat gccatccgcc
     5761 tgacgccccc gcggatcatg acgctggagc aggcgatcgc ctatatcgac gacgacgaga
     5821 tggtggaagt gacgcccaag tcggtgcgca tccgcaaggc gatcctcgat cccaacgagc
     5881 gcaagaaggc cagccgcaag gcggactgac cgcttgcccc tgccgcccca tttgggaacg
     5941 gcagagcttt gacaaaggcc ggaacggggc gacccgtttt ccggcctttg tcctgtctgg
     6001 gccctgccag tccttgccgg cagaaccctc cacacaacca ccgaaatgcc ggaagaaaca
     6061 gttgatcgca ccgcagcaat gacttcagat gtaacatcgg tcatttatcg attccgcagc
     6121 tgttaatggc atcgaccgat ggatgggggg tctgtcaatg catcgcttcg caactcttgg
     6181 ccttagcact actggcctta gcactactgg ccttaccact actagccttg ccactattgg
     6241 ccttgccgct gccctgctgg gcctgggcgg ctgcacattg ccgccagccg attccagcgg
     6301 cagcgcagcc tctgccgcgg ccgccgatgg cagttcggaa agcagcgtca gcctcgatgc
     6361 cctgctgaac cggggcacca atgtgctgac catcaagaac accagcgggg gcgcgctggt
     6421 gctgcaaagg gtcgtcgtca acggccagga aggcgccgac agctgcaaca acaagctgtt
     6481 cgtcgatatg gccaatgccg cctcgcgcga tgtgtcgctg ggcgattgcg gcgtagtgcg
     6541 ctccaccaag gtttacaccg atcacggcac ctattcctcc gactggacct atgagccctt
     6601 catcgacctg tctgtcagca gcggctcgat cacggtgaag aacctgggca tcaacaaata
     6661 tgtcgtgcag tcgatcatcc tcaatcatcg caagaatgat tccaattgcg atatcaaggt
     6721 attccgcgat ctcgatgcct ccggctcgct cgatgtctat ggcaccgacc agtgcggcga
     6781 gatcaagcat tacgaagtgg tgacggacaa gggcacgcgc gaattcggcg acgatctcta
     6841 ggggtgtggc cccggggggc gggccaaccg cccatttgtt cataaaccgt aaccgccagc
     6901 tgaacgcagg ctgcactgcc ggtgccgcct gcgttcaggc atgaaggcct ataccgcgac
     6961 tcacacggcg atctgcccct tgcgccagcc ttccctggcc gggccgccaa gaaggagttg
     7021 cgacatgatc cagttcttga agaagggcct gctggcaagc gcgatagcgg ccagcgccgt
     7081 ggccagtttc gcggccaccc cggccatggc agacccctgg cggggcggct atcgcggtgg
     7141 ctatcatggc ggcgatgcgg cgggcgcggc agtggctggc ggcatcatcg gcctggccgt
     7201 gggcgcggcc atcgcctcgg atcgcccgca ttatgtggcg ccgccgccct atgcgccggc
     7261 ctatgtggca ccgcccgcct attattacga cgagccggcc tattatgtcg atccggcctg
     7321 ggtgtggcgc gatggctggt actgggaccg catgggccac cgctactacc gcgacgggcg
     7381 cccctggggc tatgccgccg gctggcgggg tggctggcat gacggcggcc actggggcgg
     7441 ccccggctgg cgcggtggtg aacattgggg cggcggccat tggggcggcg aacatggtgg
     7501 ctggcacggc cgctgatccc cttcctgccc ggccttgaaa gcccggcccc gaatagcccg
     7561 ccccggccca ccggcccggg gcgggctttt gccatcgcct ccgccgcttt cgcccgccgc
     7621 cccctcgcca aagcggcctt gcgcggctat gggccctgcc atgaccatcc cggccaagac
     7681 agacgaactg cgtgtcgccc tcttcagcgg caattacaac tatgtgcgcg atggcgccaa
     7741 ccagtcgctc aaccggctgg tgggctatct gctgcgccag ggcgcaaagg tgcgcgtcta
     7801 ttcgcccacc atcgagacgc ccgccttccc gcccaccggt gatcttgtct cgctgccctc
     7861 gctgcccatt ccggggcggg cggaatatca tctggccacc ggcctttcgg cggcggtgcg
     7921 gcgcgatctg gaaggtttcg cgcccaacat cgtgcatatc tcctcgcccg atccttccgc
     7981 ccaccgcgcc gttacctggg cgcgcaagcg gggcattccg gtgctggcca gcgtgcacac
     8041 ccgcttcgac acctatccgc gctactataa cctgggctgg atcgagccgg cgctcaccgc
     8101 catcctgcgc cggctctatc gccgctgcga tgcgctggtg gtgccttcgg aatcgatggc
     8161 gcaggtgctg cgcgaccagc gcatgaacta cgacatctcg atctggtcac gcggggtgga
     8221 ccgcgagatc ttcacccccg cccgccgcga catggtttgg cgccaggccc agggctttgc
     8281 cgacaacgag gtggtgatcg gcttcttcag ccggctggtg atggaaaagg gcctcgatgt
     8341 ctttgccgat gcgatcgacg agctgaaggc gcgcggcgtg ccccaccgcc tgctggtggt
     8401 gggcgatggc ccggcgcgcg actggttcga gaaccgcctg ccgggggcgg tgttcaccgg
     8461 cttccagggc ggcacggatc ttgcccgggc ggtggccagc atggatctgc tgttcttccc
     8521 ttccgtcacc gagactttcg gcaatgtgac gctggaggcg atggcctgcg gcctgccggt
     8581 ggtggcagcc gcggccactg gcagcgaaag cctggtggat gatcatgtct cgggccggct
     8641 gattcgcccc ggcgccatcc atgcctatgc cgaagccctg cgcgcctatg tggaaagcgc
     8701 cgatctgcgc gctgcccatg gcgccgccgg cctggcccgg gcgcaggaat tcagctggga
     8761 ccgcatcaac cagtccgtcg ccgacactta cctcaggctg atccgccagc ggggataggc
     8821 gcgccctgcc cttctggccc cttcaatcga gataacccgc ccagaacatc cgccgcgcca
     8881 gccaccactg cacgccaatg gcgatctggc gcaccgccag cacccattgc accagcaagg
     8941 tgcagcgcgg atcgccgaaa tgatgcagca tgatctggct gtagcaggcc gcgctggcgg
     9001 caaagccgat gacaaaggcc accgccgcat ggcggctgcg cagcagcatc agcccggcgc
     9061 ccgccagcgg cgcatagacg ctggcctcgc gcaggatgag catatagagc ggcaggccat
     9121 ccagccattc caccaccggc tggaggaaac ccggcgccag atgcatctcc agcatccaga
     9181 agcgcccgac attgaagaga ttccagacaa ggcaggccca taccgccaag cggaaccggc
     9241 ccatctcctg atcgggacat ctgctcatgg cacgaattgc tcttttcccc gttcttatgc
     9301 ctaatccccc tagcaggctt tccttaaaga tttcctgttt ccggaaaatg cctgcccttg
     9361 ggggcatggc atttccggcg ccaatgccta gattggcgcc atggccgatc tgtttgcctc
     9421 tgccgccgat agcgcccctg ccgcgcccgc cctgccgggt gcgggcacag gcgctgccat
     9481 gccgctggcc gaaaggctgc gcccggccag ccttgccgaa gtggtgggcc aggaacatct
     9541 gaccgggccc gagggggcga tcggccggat ggtgacggcg ggccgcctct ccagcatgat
     9601 cctgtggggg ccgccgggca ccggcaagac cagcatcgcc cgcctgctgg ccgcggccgt
     9661 gggcatgcgc tatgcgcaga tcagcgcggt gttctcgggc gtggcagacc tcaagaaggc
     9721 ctttgccgag gccgaagggg cgctgtcgct ccagggcagg cgcacgctcc tgttcgtcga
     9781 cgagatccac cgcttcaacc gcgcccagca ggatggcttc ctgccctatg tggaagcagg
     9841 caccgtaacg ctggtgggcg ccaccaccga gaaccccagc ttcgcgctca acgcggcgct
     9901 gctcagccgc gcgcaggtgc tgatcctgca ccgcctgccg gcagacgcgc tgacccgcct
     9961 gatgcagcgc gccgaggaag ccgaaggcgt gcccctgccg ctgaccccgc ccgcgcgcga
    10021 ggcgctggtg gcctcggccg atggcgacgg gcgcttcctg ctcggccaga tcgagacgct
    10081 gtatggcgcc agcctgttcg cgccgctcga tcctgccggc ctgggccagt tcctgcagcg
    10141 ccgcgtggcc gtttacgaca aggaccgcga cgggcattac aacctcatct cggcgctgca
    10201 caaatcgctg cgcggatcgg atccgcaggc cgcgctctat tatctcgccc gcatgctcac
    10261 cgccggcgag gatccgctct atgtactgcg ccggctggtg cgctttgcca gcgaggatgt
    10321 ggggctggcc gatccggcag cgctgcccca gtgccttgcc gcgcgcgacg cctatcagtt
    10381 cctcggcagc ccggaaggcg agctggcgat cgcgcaggcc tgcctttatc tcgccaccgc
    10441 gcccaaatcg aacgctgcct atgccgcctg gaaggaggcc gccgccaccg ccaaggccac
    10501 cgggtcgctg atgccgccgg ccaatatcct caacgcgccc accaagctga tgaaggagat
    10561 cggctatggc cagaactatg cctatgacca cgacgccgag gatggctttt ccggcgccaa
    10621 ctactggccc gaaggcatgg ccccgcaaag cttctaccgc ccggtggagc gcggcttcga
    10681 gcgcgaggtg ctgaagcggc tggactggtg ggagaagaag cgcagggaac gccagcaggg
    10741 cggataggcg cacaggtgcc cccccgcttc acccatttcg ccgcgataga ctggtctggc
    10801 gcggcaggca ccagccacaa gggcattgcc gtggccattt gcgcggccgg cgatgccgcg
    10861 cccgttctgg tggcgccccc ttccccgcgc ggctgggcgc gcaccgaagt gctgcgctgg
    10921 ctggtggaga gcgcgccccc cacaacgatg atcggcttcg acatggggat cagcctggcc
    10981 catgccgatt gcggcgcctt ctttcccggc tgggcgcaaa gcccggccga tgcccgcgcg
    11041 ctgtgggcgc tggtggagga gatctgcgcg ggcgagccgg atctgggcgt ggcgccgttt
    11101 ctcgcccatc cgcaggccgc gcggcatttt cgccagcagg gtggccggct gggcgatctg
    11161 ttccccacca gcggccccac cggcgcgcgc ggccgctggc gcgtggccga ggaagcccag
    11221 gccgccatgg gctgcgcgcc caccagcaat ttcaacctgg tgggggccgc gcaagtgggc
    11281 aaggcctcgc tggcggggat gcgcctgctg caccgcctgc cagacagcat cgccgtgtgg
    11341 ccggtggacg aggtggcccc ccgccgcaag gtggtggccg agatctacac cgccattgcc
    11401 gccattgccg ccggccggcg ggcgggcgcc agcaagatcc gcagccacga ggcactggcc
    11461 gaagcgctgg cccaccccgc catcgccagc gccgcgccca caggcaccgg ccccatcgcc
    11521 gatcatgccg ccgatgccct gctgacagcc gcctggctgc gccgccacgc ccatctgcca
    11581 gcgctgtggg cgccggcccg cctcaccccc gcgcttgccc acaccgaggg ctggaccttt
    11641 ggcgtggcat agggcatggg gagcccctcg ccggcttagc acagtggtag tgcagcggtt
    11701 ttgtaaaccg aaggtcgggg gttcgaatcc ctcagccggc accagccccc ctcaccacgc
    11761 gcacaatccc cgcaaggcaa tagcctgcgg gcttggcttt gcgtgggcga tgcgccgttc
    11821 aaatcgggca tctgggccaa tgatggggaa aaagtggcag gagtggaggg actcgaaccc
    11881 ccggccctcg gttttggaga ccgatgctct accaactgag ctacactcct gcgggtgaag
    11941 gcggccctta aagacacatc gcgcccatgg caagccgcat tggcgccaac atgcgaaatt
    12001 tcccgcatct ggcgttggcg gcaggggctg ctatgctggc gcctgtgtcc aaccggcccg
    12061 cccatgcgtt gatcgacctg ccgctgctga tgctggccta tcgcagcggc atcttcccca
    12121 tggccgatgc gcgcgatgat cccgaagtgt tctgggtgga gcccaagcgg cgcggcatcc
    12181 tgccgctcga tggcttccac tgcgcgcgct cgctggccag ggtgctgaaa cgcggcacct
    12241 tcaccgtcac ctgcgacgag gcctttgccg atgtgctggc cgcctgcgca gcgccacgcg
    12301 ccgaaagccc cgacacctgg atcaaccagc gcatcgcggc cacctacatt gccctgcatg
    12361 ccgccggcca tgcccattcg ctggaatgct ggcagactgg tgcggatggc cagcggcggc
    12421 tggttggcgg cctgtatggc gtgggctttg atcgggtgtt ctgcggcgag agcatgttct
    12481 cacgcgtcag cgatgcctcg aaagtggcgc tggcctggct ggtggcggcg ctgagggcgg
    12541 gcggctatcg cctgctcgat tgccagttcc tcacagatca cctgggcagc ctgggggcgg
    12601 tggaaatcag ccagaagcgc tatctggaac tgctgcgcgc cgcgcaaagc ccggaaagcc
    12661 cccatgcggg cgtggatcag cggggcgcct cgggcgtggg cgcggcttcc ggcgctgcgg
    12721 gcacaggcgc cggctctgcc gatgcggccg aggaggcgct ggcgctgccc gaggcctttg
    12781 gcgccttgct ggcggcggca aaatcggcgg ggttctcttc ctcgccgggg aacttcatcg
    12841 cgcaatcctt cacccagaca tcatagaccg ggtgctccac cacattgagc gaaggcgcgt
    12901 tgcggaacag ccagcccgag aagaccttgt gccaggccag cggatcggac agcctggccc
    12961 gctcctccac gaaaacctgc acgaaggcgc cggtttcggc cgggcgttcc cagggcaggg
    13021 tgcgctcgca ggtggccagc ttcaccacca cattgcccac ccggcgcgat tcgcccggct
    13081 tcatcaccag atcgaccgat tcgttgttgc gcttgttgag caggccaagg gtggccaccc
    13141 tgtccttcac cggcgtgcca tactggcttt ccaccgccgg gttgggctgg ccctggccgg
    13201 ccacatcctg cggcacgtcg gtcggctggg gggcgggcgc atcgccgccc cggtggcagg
    13261 cggccagcgc cagcacgccc acactcagca gcgccgcgcg catcaggcca tcgcggcgca
    13321 tgggaaagat cagccttccg gggtccaggc ttcgtagtcg ccagtggcag cggcgcgctt
    13381 gccgcccttt tccagcgcgc cggcggggcg ataggcggcc acggtgccgg tggcgttggg
    13441 ggtatattcc acttcccaga tgcgcggcgg cggcaggtgg ctttcgggca cgccatcata
    13501 ggtgccatgc agccagccat gccattcggc cggcacgcgg ctggcatcat tgttgccctt
    13561 gtagatcacc cagcggcgct cgcgcccggc aaaagcgctg ttggcgccag cggggatctt
    13621 cttgcggctg cggtaatagg tgttgccctg atagtccgtg ccaaccagtt cgccgttgcg
    13681 acgggtgttc agccaggtgc cgaaagtggc gccattccac caggtgaaga gcattgccgc
    13741 gaaatccttc ctaacgctgc cggcgggccc tgcacgcagc gcgcaagacc agccacgccc
    13801 ccgcgcctag cggatcaccc cgccccagag caagtgcgat ataccccttg ggcggctgcg
    13861 aataaatcat ttgccggcaa aaaggggcag ccgggcggtg gttcattccc acaaatcggc
    13921 ggcggggccg tcatcggcgg ggccatcgcc ctcgtcggca gcgccatcgc cggccgccca
    13981 gccctgcgct gcagccacat cgggcgaatc gaccagcgcc cgcgccacgc caaaaccatg
    14041 ccccgcccgc agcagcgcgg cgatctgctt ctcgcgccgg cgcggatcct ccttctgccc
    14101 ctcccgcgtg aaggggccaa ggccgcggcg ccgagcaaag gccagggcgg cggcccgggc
    14161 ggcgctttca tcggggcgca ggccggcgcg gatctcctcg tcgatcccgg ccgcatccag
    14221 cgcctgatcc acccgccgcg cgccatagcc gcgccgcagc aggctggcgc tgcgggcgcg
    14281 ggcaaaaccg gcatcatcga catagccggc cgccaccgcc cgcgccacca gcgcggcgat
    14341 cacctcttgc gcggcggcca gcgccgcctc gccggccagc ggctcgccct gccctgccgc
    14401 ctgccagccc gcccggcgca ccttgccggc cagataggta tccagccgcg
//
LOCUS       DASRYE010000185         5204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_10931,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000185 DASRYE010000000
VERSION     DASRYE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10931"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFF98_09655"
     CDS             complement(<1..438)
                     /locus_tag="VFF98_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU63935.1"
                     /translation="MTTNRFWQLDAHPKGHDFAAALSLREEPLAPLAEGEVRIASQYL
                     SMDAGTRMWMSPRTDGYQPPLPLGAKMVGLVLGHVCESRDPAFPAGALVRGFGQWASH
                     AVVVPALAGLEIVDESVADIRQHFGALGMNAWTAYVGVKEVAAV"
     gene            610..3072
                     /locus_tag="VFF98_09660"
     CDS             610..3072
                     /locus_tag="VFF98_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63936.1"
                     /translation="MAQNKYALLLGSAIASGLFAQPLMAAEPAAAPTDAAQPVKENSG
                     LSDIIVTARKRTESAQSIPVAVTAISAKTIEQRDLTSIEKIAAATPNLTVGHSSNGSA
                     AQVTLRGIGSSSTSIGIEQSVATVVDGVYYGQGRVLEEGFFDLAGVEVLKGPQVLFFG
                     KNATAGVISIKTADPTPDWQFKTRASYEFKGEEAQLEGVASGPLTQNLGIRVAVRGTK
                     AWGGYYDNESRPYSITYPLSATTSPTYTSYPTSSNQPGNREFLGRITLKWQPTAEITD
                     TLKASYDYNKANNSSYNYVAYNCPGGVTQLSGYACGFNFTTHQNNLPSQVAANFPYAG
                     DGSLYNQYKSAAVTNTFNIDLGKVTLTNVTNYNWNNNQWLCACEFDSSASSVWATENS
                     SWSAFSNELRALTHYDGPLNVMMGLLYQSTKRDFAQYVSYSYNNDPTQGANQYMDGTK
                     MSWTKGETISGFGQVTWKVLPKVELDAGVRYTHETKDSYFIQPYTSPTFIGAWPDGSA
                     SVVNAHQVFDNWSPDVSLTYKPVRDVMFYAAYKTGYKSGGFSNGGIYSLAVTNPASAF
                     TFNPETTAGFEVGVKSTLADNQLRLNLNFYTYKYKNLQIDFFDSAKVAFQTLTADART
                     KGVELEAEYAPRAVHGLNVHGAINYNDAHYTSFPAAPCYAGQSISEGCNENADGSPIA
                     SGGTGVRQNLNGVVLGMAPHWTGTLGATYETAVGNRYKAGISADMRYSSGYLVSGFGE
                     YFSRNPSYAVLDMGVRFGAEDGNWQVAVLGKNLTNKQYINGGVDGPLTGGGTGTTSGT
                     HADLMGFGALPRTVTLQISKNF"
     gene            complement(3243..3653)
                     /locus_tag="VFF98_09665"
     CDS             complement(3243..3653)
                     /locus_tag="VFF98_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU63937.1"
                     /translation="MPFTGPVEDRLAIRELLETYADAVTQNNAEAWGGTWAEDAEWSL
                     PDYPEIGTTKGRAAIVAMWVEAMKHYPGIMFEAWPGSVEIDGNTARMRSYTSEVYEQE
                     GATKRDRGIYTDELVKLDGRWFFKSRSFRNIHRQ"
     gene            complement(3756..4106)
                     /locus_tag="VFF98_09670"
     CDS             complement(3756..4106)
                     /locus_tag="VFF98_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZU63938.1"
                     /translation="MSDIWHPLIAEADFPEGGKLATKVGGWHVLVALTDDGLHAVNDR
                     CTHQAALLSSGRIRRGAVMCPLHGARYEMATGRCIGGAYKDLRPFPVRVEGGMIEVAV
                     PEAPPTMDELPVGI"
     gene            complement(4116..4664)
                     /locus_tag="VFF98_09675"
     CDS             complement(4116..4664)
                     /locus_tag="VFF98_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU63939.1"
                     /translation="MSLAAALFSHPVRQIAYFCADVRAAATRHHALFGSGPYFVADHI
                     PLGASVHRGAPQAFDHSSAYGQWGEVMVEFMQQNNPGPSACHDLYPEGSGRFGLHHLA
                     LWVEDVDAEVARLAALGFPLAQRSQMTDGFTFAFVDTSAMLGHMIELYTPTDALTGFY
                     AMVAKAARAPGDRPLLTTISMR"
     gene            complement(4661..>5204)
                     /locus_tag="VFF98_09680"
     CDS             complement(4661..>5204)
                     /locus_tag="VFF98_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63940.1"
                     /translation="PWLDVLVNNAGITTLGSIEEVTLAQLRHEFDVDVAGVFLGCKHV
                     IPLMRAERPDGAPTGGSIINMSSMAGLRAQGHLVAYNAAKAAVTHLTKSCALHYAERG
                     YNIRCNSVHPGAIHTPIIDKVLAQSDTPEALYASFVAVHPVGRLGRPEEIAAIAAYLA
                     SDESAFATGAEFRIDGGSTL"
BASE COUNT          971 a         1673 c         1689 g          871 t
ORIGIN      
        1 caccgccgcc acttccttca cgccgacata ggccgtccac gcattcatcc ccagcgcgcc
       61 gaaatgctgg cggatatcgg ccacgctctc atcgacgatc tccagcccgg caagcgctgg
      121 caccactacc gcatggctgg cccactggcc aaagccgcgc accagcgcgc cggccggaaa
      181 ggccggatcg cggctttcgc acacatggcc cagcaccagc cccaccatct tggcgcccag
      241 cggcagcggc ggctgatagc catcggtgcg cgggctcatc cacatgcggg tgccggcatc
      301 catcgagaga tactggctgg cgatgcgcac ttcgccctcg gccagcggcg ccagcggctc
      361 ctcgcgcaag gagagggcgg ccgcaaaatc atgccccttg gggtgggcat cgagctgcca
      421 gaaacggttg gtggtcatcg cggcatcctt ggctgaaaaa ggctgttttc cggcgatatg
      481 gggtgggctt gtggctctgc gatcacacct aggcaaaggg atagggctaa ctgtttgtcg
      541 aggggtttgc gatccggcgc ggtggcatgg ccggcatcaa cgtgatgtga aaatgggagg
      601 agcctgaaca tggcccaaaa taaatatgcc cttctgctcg gcagcgcgat tgcttccggg
      661 ttgttcgccc agccgctgat ggctgccgag cctgccgcag cgcccaccga tgcggcccag
      721 ccggtgaagg aaaacagcgg cctttccgac attatcgtga ccgcgcgcaa gcgcaccgag
      781 agcgcccagt cgatccccgt ggcggtgacg gcgatctcgg ccaagacgat cgagcagcgc
      841 gatctcacca gcatcgagaa gatcgccgcg gccacgccca acctcaccgt cggccactcg
      901 tcgaacggtt cggcagcgca ggtcaccctg cgcggcatcg gctcctcctc cacctcgatc
      961 ggcatcgagc agtcggtggc aacggtggtc gatggcgtct attatggcca gggccgcgtg
     1021 ctggaagagg gcttcttcga cctggccggc gtggaagtgc tgaagggccc gcaggtgctg
     1081 ttcttcggca agaacgccac cgccggcgtg atctcgatca agaccgccga tccgaccccc
     1141 gactggcagt tcaagacccg cgccagctat gagttcaagg gcgaggaagc ccagctggaa
     1201 ggcgtggcct cgggcccgct gacgcagaac ctgggcatcc gcgtggccgt gcgcggcacc
     1261 aaggcctggg gcggttatta cgacaatgaa tcgcgcccct acagcatcac ctatccgctc
     1321 tctgccacga cgagcccgac ctacacgtcc tatcccacca gcagcaacca gccgggcaac
     1381 cgcgagttcc tcggccgcat cacgctgaag tggcagccca cggccgagat caccgacacg
     1441 ctgaaggcct cctacgacta taacaaggcc aacaactcca gctacaacta cgtggcctat
     1501 aattgccctg gcggcgtgac ccagctgtcg ggctatgcct gcggtttcaa cttcaccacc
     1561 caccagaaca acctgccctc gcaagtggcc gccaacttcc cctatgccgg cgatggcagc
     1621 ctctataacc agtacaagtc ggccgcggtc accaacacct tcaacatcga ccttggcaag
     1681 gtgacgctga ccaatgtgac caactataac tggaacaaca accagtggct gtgcgcctgc
     1741 gagttcgact ccagcgcctc ctcggtgtgg gccacggaaa attcgagctg gagcgccttc
     1801 tcgaacgaac tgcgcgcgct tacccattat gacgggccgc tcaacgtgat gatgggcctg
     1861 ctctatcaga gcaccaagcg cgatttcgcc cagtatgtga gctatagcta caacaacgat
     1921 ccgacccagg gcgccaacca gtatatggat ggcaccaaga tgagctggac caagggcgag
     1981 acgatctccg gctttggcca ggtgacctgg aaggtgctgc ccaaggtgga gctggatgcg
     2041 ggcgtgcgct atacgcatga aaccaaggac agctatttca tccagcccta caccagcccg
     2101 accttcatcg gggcctggcc ggatggcagt gcctcggtgg tgaatgccca tcaggtgttc
     2161 gacaactggt cgcctgatgt ctcgctgacc tacaagccgg tgcgcgacgt gatgttctat
     2221 gccgcctaca agaccggcta caagtcgggc ggcttctcga acggcggcat ctactcgctg
     2281 gcggtgacga acccggcctc ggccttcacc ttcaacccgg aaaccaccgc gggcttcgaa
     2341 gtgggcgtga agagcacgct ggccgacaac cagctgcgcc tgaacctcaa cttctacacc
     2401 tacaagtaca agaacctgca gatcgacttc ttcgattcgg ccaaggtggc cttccagacc
     2461 ctgacggcgg atgcccgcac caagggcgtg gaactggaag cggaatatgc gccgcgagcg
     2521 gtgcacgggc tgaatgtgca tggcgccatc aactacaatg atgcccatta caccagcttc
     2581 ccggcagcgc cctgctatgc cggccagtcg atcagcgagg gctgcaacga gaacgccgat
     2641 ggttcgccga tcgcctctgg cggcaccggc gtgcgccaga acctgaacgg cgtggtgctg
     2701 ggcatggccc cgcactggac cggcacgctg ggcgccactt acgaaacggc cgtgggcaac
     2761 cgctacaagg ccggcatcag cgccgatatg cgctacagct cgggctatct ggtttcgggc
     2821 tttggcgaat atttctcgcg caatccctcc tatgccgtgc tcgacatggg cgtgcgcttc
     2881 ggtgcggaag atggcaactg gcaggtggcg gtcctcggca agaacctgac caacaagcag
     2941 tatatcaacg gcggcgtcga tggcccgctg accggtggtg gcaccggcac caccagcggc
     3001 acccatgccg acctgatggg ctttggcgcc ctgccgcgca ccgttacgct gcagatcagc
     3061 aagaacttct gatctgcaac gcaaggggtg gccctgaaca aagggggcgg tgggcttcgg
     3121 cctgccgccc cctttggcgt tggggggggc aggggcggcg ggccagcgcc agcgccccaa
     3181 caaaaaccgc cgccccggca agggagcggc ggcttgagac ccggcgaggg tgccggtgat
     3241 gattattggc ggtgaatgtt gcggaagctg cggctcttga agaaccagcg gccgtccagc
     3301 ttcaccagct cgtcggtgta gatgccgcgg tcgcgcttgg tggcgccttc ctgctcgtag
     3361 acttcggagg tgtagctgcg catgcgggcg gtgttgccgt cgatctccac agagcccggc
     3421 caggcctcga acatgatgcc gggatagtgc ttcatcgctt ccacccacat ggcaacgatg
     3481 gccgcgcggc ctttggtggt gccgatctcg gggtaatcgg gcagcgacca ttcggcgtct
     3541 tcggcccagg tgccgcccca ggcctcggca ttgttctggg tgacggcatc ggcataggtt
     3601 tccagcaatt cgcggatggc gagccggtct tcaacgggtc cggtaaaggg cattgtcctc
     3661 tccttgtgca atgcgttgtg catcgggttg gccgccagcc tagcgccctg ccaaagggcg
     3721 ccaagctgtg gctaaggaga ggtgcgcttg tcgtttcaga tgccgacggg cagttcgtcc
     3781 atcgttggcg gcgcctcggg cacggcgacc tcgatcatgc cgccctccac tcgcacgggg
     3841 aaggggcgca gatccttgta ggccccgccg atgcagcggc cggtggccat ttcatagcgc
     3901 gcgccatgca gcgggcacat taccgcgccg cggcggatgc ggcccgagga aagcagcgct
     3961 gcctgatggg tgcagcggtc gttcaccgcg tgaaggccat cgtcggtaag ggcgacgagc
     4021 acgtgccagc ctcccacctt ggtggccagc ttgccccctt cggggaagtc tgcttcggcg
     4081 ataagcggat gccagatgtc ggacacggat actccctagc gcatggaaat ggtggtgaga
     4141 agcgggcgat cccccggcgc gcgggcggcc ttggccacca tggcgtaaaa gccggtcagc
     4201 gcgtcggtgg gggtgtaaag ctcgatcatg tggcccagca tggccgatgt gtcgacgaat
     4261 gcaaaggtga agccgtcggt catctggctg cgctgggcga gggggaagcc caaggccgcc
     4321 agccgcgcca cctcggcatc gacatcctcc acccacaggg ccaggtgatg caggccaaag
     4381 cggcccgatc cctcggggta aaggtcgtgg caggccgagg ggccgggatt gttctgctgc
     4441 atgaattcca ccatcacctc gccccactgg ccataggcgg aggagtggtc gaaagcctgg
     4501 ggcgcgccgc ggtgaacgga ggcgcccagc ggaatatgat cggccacgaa ataggggcca
     4561 gagccgaaca gggcatggtg gcgggtggcc gctgcgcgca catcggcgca gaaataggcg
     4621 atctggcgca ccggatggct gaagagggcg gcggccaggc tcacagcgtg ctgccgccat
     4681 cgatgcggaa ttcggcgccg gtggcaaagg cgctttcatc gctggccaga taggcggcga
     4741 tggcggcgat ctcttccggg cgccccagcc ggccgaccgg atgcaccgcc acgaaggagg
     4801 catagagcgc ctcgggcgtg tcggactggg ccagcacctt gtcgatgatc ggcgtgtgga
     4861 tggcgccggg atgcaccgaa ttgcagcgga tgttgtagcc gcgctcggca tagtggagcg
     4921 cgcaggattt ggtgagatgg gtgaccgccg ccttggccgc attataggcc accagatggc
     4981 cttgcgcgcg caggcccgcc atcgagctca tgttgatgat cgagccgccg gtgggcgcgc
     5041 catcggggcg ctcggcgcgc atcagcggga tgacatgctt gcagcccagg aacacgccgg
     5101 ccacatccac gtcgaactcg tggcgcagct gcgccagcgt tacttcctcg atgctgccca
     5161 gcgtggtgat gccggcattg ttcaccagca cgtcgagcca gggg
//
LOCUS       DASRYE010000186        25258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_205271,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000186 DASRYE010000000
VERSION     DASRYE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 25258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25258
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205271"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFF98_09685"
     CDS             complement(<1..340)
                     /locus_tag="VFF98_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HZU63941.1"
                     /translation="MSGLEMVAVAVTALAIWLTARRHMLCWPLNVLACALYFKLFLDV
                     RLYADMVLQALFGLAILYGWREWARAPAQAGEAAGPVAVAPLPAAQALAALAAGALGA
                     LAIGWFTSHYT"
     gene            complement(337..1473)
                     /locus_tag="VFF98_09690"
     CDS             complement(337..1473)
                     /locus_tag="VFF98_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZU63942.1"
                     /translation="MAEGHLVHGLGTGLAAPAWPPVTPAEAAAVLARVPGAGVLEALE
                     WHSPRPFSAAALARTSQGELFLKRHSPALRSPAGLAEEHAFIAHLRQAGLPVADVLAD
                     AAGASAFALGGWSWEVHRKSAGEDLYRERPSWTGFLCPAHAHAAGAMLGRLHRAAAGF
                     AAPARAPAPLVAGNTLFAGGDPQAAAEAFVARWPALGAYFAGTGWRAELAALLAAADA
                     AGLSRALGQRPTLWGHGDWHPSNLLWDGAGGVAGIMDFGLADRTCALFDLAIALERSV
                     VAWLQLGKGEDAALADLPCADALLAGYASAAPLTGEDRALLARLLPLVHVEFALSEMV
                     YFEGILALRADTDLAWHTYLLGHARWFAGAGGQALLAATRGAAT"
     gene            complement(1483..3735)
                     /locus_tag="VFF98_09695"
     CDS             complement(1483..3735)
                     /locus_tag="VFF98_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63943.1"
                     /translation="MGLGLPGAALAEGEEGTDHTPIRVEGHPDPEGLLPAQTVPVAVS
                     AIGSAFIAKQAPTLNAFQLVNLLPGANVAMSDPYGLSTSNSLTLRGLTQDEIGVLMEG
                     APQNDIGYYDAYPSQFADAENLRSVALAPGAVDLDAPVVNGAGGLLSLSLDDPHRRMG
                     LAATISAGSYNMRRFFVRLDTGEIGNSGIAAFVSYSNTYADNWRGAGYDVRRHIDGKI
                     TKHWGEGNSAAIAVSFNDARNSTYPNPTLADWQAYGRGYNYDATYTAGDTAYWRLYRQ
                     PFRNTYLSAPLHLQLGEGAAFDSTFYFQRGYGNSPYGTQLDSTGNYLGTEALGAISLP
                     GGASSATVLGNWLGDQLRTGDVSKLTVTLGAHRLTAGLWFDYGTDRDVESFTALDGNG
                     NPLDAWGYDANAIHTADGRLLAIENIRTTTVVKGFFLADHIALTGRLGADIGFKGVDV
                     LHNGHNYLPGDQSAVHTDSFAALPRAALSWRASDAHQFFANLSTHFRAPDEYTLYDGY
                     DGYGDVTQRGTTALKNEYAISEELGWRYTGAVLSASLTAFHYHFRNRQLATLADVDGA
                     LINTTMNGGAQTSYGLDGEIDWRPIAGLSFYASGEWLHARLDDNLPVDGDYLPTRGKQ
                     AVSSPAVQAALGGTYDRGRLFGSFALKYVGKQYATFMNDEHIAAYATLDLSVGVHLAG
                     HGTGEAPHTDLRLNFLNVTNPHVLSGVAGLSTNARDTTGLNGTVIAGSAPTYYIGGGA
                     AVVASLSRQF"
     gene            complement(3964..4668)
                     /locus_tag="VFF98_09700"
     CDS             complement(3964..4668)
                     /locus_tag="VFF98_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU63944.1"
                     /translation="MSARQEPARAGAERAASTSAMQAYRQIRSAILAGRFEPGQFVPE
                     EEFAQLCGMSRTPVREAIARLVSELLLQRTDTKRLFVPSWSDDEIEELFALRAGLEAH
                     CARRTASLITPAEIEELRGHAARIDAAIADPQAIDVPAFVEGNRLFHAAMMAAARSDR
                     LAQLMRLVVSQVIVHRTAERYTHEDMVRSQADHLDLIRAFAAHDGEWAAAIAANHIRR
                     AANAYRAHQAAAPAAC"
     gene            complement(4790..5788)
                     /locus_tag="VFF98_09705"
     CDS             complement(4790..5788)
                     /locus_tag="VFF98_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZU63945.1"
                     /translation="MALPLLGSASLAQATEPTPAQTTEPASPAPAASPAPATSPVTAS
                     PALPPAAPAEPAVAGAPKPIVVSGKTWADRQDPARKLNETSFQAVQATDRAFIGPAGR
                     LYKRGVPKPLRDGFHNVLYNAREPAIAVAFLMQHRIGKTGETLGRLVLNSTLGLGGLF
                     DIARRKPFRLPYRENGLADTMGFYGVKPGPYMFVPLMGSTTLRDLVGTTADRLMLPTI
                     IGGALNTIWVDATITVVNGLDDRMTQDRNGGRDWEKAPNPYRAKRAIYLREREAEIAA
                     LHTHKHKPSAPAAPAPAPAPAPAPAPAPAAPALAPATPAPTPAPSPAPTPAPTPAP"
     gene            complement(6117..7133)
                     /locus_tag="VFF98_09710"
     CDS             complement(6117..7133)
                     /locus_tag="VFF98_09710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU63946.1"
                     /translation="MLATKQNVLRRFWYATVRLDDLAAGPRPFTLLGERIVLFLGADG
                     QPAALEDRCPHRTARLSLGWCDKGRVICGYHGWEFDSEGHLARIPQFTEGQALPNAQI
                     RKFHAQARYGYVWVALEQPLADIPAVPEDEDPAFRRIYQHYGRWECSALRLMENSFDQ
                     AHIAFVHKGTFGEIDRPRPTRYAIEETDEGFWAETEVRVANPPRAHRVTGDTGEWTVR
                     TMRNAWYMPFCRRLDITYPSGRRHIIFNCATPIDDGAIQVTQLLFRNDTEADCPEQEL
                     IDWDAEVLLEDRTMLESTDPDAVLDLARKAEMHMPSDHPGLIMRKRLLALLAAHGEEE
                     VAAA"
     gene            7446..8432
                     /locus_tag="VFF98_09715"
     CDS             7446..8432
                     /locus_tag="VFF98_09715"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU63947.1"
                     /translation="MAALAVALSLWLARGGPAPSVHKRFVLQLSWRAQPEVGGYVEAG
                     ARGYYQACGLDVELRQGGAGIDPAQLLVAGAVDAALLPQADGIIHMNAAGFPARALFA
                     AMQHSAMALDVHADSPIRAIPDMVGHPIMLSASSRASWWPFLKRRFGFADAQLRNFTG
                     QFAPFVADPDAVTQDMISNGPYQLETKSHLQVRTFSLAPWGYDPYGAILTVSQQTITR
                     DPAAMRCLVSASQRGWQSYLADPGHAYGAIHAMSPEYTDGASRYAYKVLMENHIFSTP
                     ETDRQGLGIMSAARWRAHYALLRDLGVAPAGLDPDSLFTNAFLPGAAPAPHS"
     gene            8449..9867
                     /locus_tag="VFF98_09720"
     CDS             8449..9867
                     /locus_tag="VFF98_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU63948.1"
                     /translation="MNTPPLSTCPYPIAEISAALGDIEHTTDPILIRRKSQDRNAVSP
                     LLRQMMADKRADMIVTPKTLEEIRRTVQAAVRHQTPITVRAGGTANYGQSVPLKGGIV
                     LDMTAYAGITRLEPGLLRARSGTLTADIDTEARKIGWEMRFPPTTKQGATIGGHVAGG
                     QGGPGSVVYGTWRDRGNLVGASVLTVEEEPRVIELTGRDAQFIHHTYGATGIILEIEI
                     PLAPAFPWIEAVVAFPDYMQAVRFGIDLANAAGIIRKVVSVQGWPTPWLFKPFRAFVP
                     EGHAIVSTMIGQQTWADFAQMVAEMGGQIVAEAPEGQGPYKAPLTEFVFGHALVQIQK
                     TEPKRAVLEGFFRDTADLAGLIERVYHKVGHHGPLRVELIRISGELVGTGAPYFLYES
                     PEQMAGIVREMQEAGALVSNSHTSNVRAVGKKEITARDLAFKRAVDPHGLLNPGRFEV
                     EESADASFALALPTDSWDRRLG"
     gene            9867..11549
                     /locus_tag="VFF98_09725"
     CDS             9867..11549
                     /locus_tag="VFF98_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU63949.1"
                     /translation="MDSAAPFPLPPLRAPLPVREVEVCTQLLADGTRLDADVYRPDAP
                     GDYPMLLQRQAYGRRIGCTLCYAHPAWYAAQGYVVVVQDVRGRGSSTGVFHPGLHEAA
                     DGAAAVEWAARLPGANGRVGSFGFSYQAYNQLLAATGNCPSLVAMVPAMGPWDAARHW
                     MFENGALRLKQAVGWGVQITAEGARRVGDAAAYAELAAAGLPVEGPVSARPDVLMRHA
                     GLSHFAEWIGRGADDPAWAAISPAAQAQALAARALPTLLIGGWFDTHLASTLAAARAL
                     AGPATRLLVGPWAHFPWGRKTGQIDFGPAAALDTDRLQIAWFNRWLKEEDNGIDALPP
                     LRLFDMGQPAGQTRWRDFATMPPATVPLFTSGDGHAAIRPEAGQLQPQPPAPMAERLV
                     HDPWRPAPAVGGSHGTPPGPVDRAGVESRGDVLTFTSPAADKAWLLAGEIAAALTLGA
                     DQPGFDVACTLSRVDGQGRSFQLAAGYMRVPAGAGLREIRLPLGATCATLQAGEALRL
                     SIAGAAFPAFPVNPGSGADPAATPRAEARIITLTLHCGGAAPSLLLLPETAA"
     gene            11546..12442
                     /locus_tag="VFF98_09730"
     CDS             11546..12442
                     /locus_tag="VFF98_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU63950.1"
                     /translation="MTPPKATDALHEAQMLSSPPEPAAEAPAPAPAPPARKRTDMADR
                     WLPLAGGLGFLALWQVAVKVLAISPFTLPPPSAVFVALWQDGPELLHALAFTAAITLS
                     AFLLALASGVMLGVALTQNRRIERMLWPYAVALQVTPMVAIAPLVVIWVGLSRAWLAL
                     LVLAWVVAFFPILSNTVLGMKSADHGLKNVFTLYRASRWQRFCHLQLPAALPYIIAGM
                     RVSAGLSVIGAVVAEFVAGSGGTTGLAWVITQSGSMLDIARMFAALVVLTAFGLAMNM
                     ATGLIQRLLLGAWHESAIEQEN"
     gene            12446..13252
                     /locus_tag="VFF98_09735"
     CDS             12446..13252
                     /locus_tag="VFF98_09735"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU63951.1"
                     /translation="MQPHPQGSPPPPLVRLAHVGKVYDSGTIALADLTLDVAPGEFIS
                     LLGPSGCGKSTALRMIAGLGRHSSGLIDWPTSAYDAAGEPEREIGFVFQEPQLMPWAT
                     IAENVRLPLKLARVPRARAQARVAETLEMVGLSAFADAYPRQLSGGMKMRASIARALV
                     TEPRLLLMDEPFAALDEITRTRLSNDLLEIWQRTGCTVLFVTHSVYESVFLSTRILVM
                     APRPGRVIGEVAIDHPGPRDTAWRQSPAYQAGCRRVSALLAEAMGQPAPA"
     gene            13311..14222
                     /locus_tag="VFF98_09740"
     CDS             13311..14222
                     /locus_tag="VFF98_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63952.1"
                     /translation="MLHARLLTYIDEVVRQGSIRRAAEKLNVASSAISRQILALEEQI
                     GAPLFDRSGRRLRPTAAGELLVRHVRETLKEMTRTQAMIEELKGLRRGSVSIGLMSGL
                     AANILPRAVNDFHESNPRVEVRLKLMTTGDEILNAVEKGEVALGLGFDFPRHAGVRVV
                     HAATGRLGAVMNPEHPLAGQSELRLRDCAPYPLVLADPTTAIRPHIDEAFARSGFTAL
                     AFTETNSIEMMRQMAILGRSITFLTPFDIEFEQRMGRLTYVPMPELARHEQRLMLVQN
                     ERRSDALAGLLAERLKALIHSVAETGG"
     gene            complement(14268..14690)
                     /locus_tag="VFF98_09745"
     CDS             complement(14268..14690)
                     /locus_tag="VFF98_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU63953.1"
                     /translation="MDHFVARSVADVAAFRISPKDTNYFALLFDPEGDGIEPVCVVEI
                     FNVGGRTPPNSHAIAHEFFYVLHGQGMAHCAGASRAIAKGDALMLRPGAEHVIENTGA
                     TRLYTLTFMVPNEGFAELIRAGEPVALDAEDLAVLTKG"
     gene            complement(14705..16330)
                     /locus_tag="VFF98_09750"
     CDS             complement(14705..16330)
                     /locus_tag="VFF98_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZU63954.1"
                     /translation="MTGETAGPLPGLTSASMTTRDGIRLDADIWRPTTGTGPWPVLLM
                     RQAYGRRLGSSLCYAHPAWYAGQGFMVVVQDVRGRGTSEGVFDAFRHEGADGAEAVEW
                     AAGLAGSNGHVGLFGFSYQGTLQLLAAAHRPPALKAMAPAMVGWDLTADWVSEQGAMR
                     LAPNLGWGVQMAALSALHQGEVAAFEKLASAARGLPLAGAVPGRPEALATELALGHYP
                     AWRDTPADDPFWAGLSPAAQEPAIAAAGIPALFVGGWFDTHLIGTLAAHKALGGTLIV
                     GPWTHFPWDRLVGERDFGPEAAHPIDAAQVAFFRYWLAGEGAPPAPLQWFDMGTLGWC
                     HELAAGEVVLHLAGDGRVSVATQSGHLLADPAAPAVDYLVHDPWRPAPACGGAFATPA
                     GPVDRARHDQRADVLTFTTAPMAAPLAIAGAVSASLSLLADAPSCDIACTLSRIDAEG
                     RVWQIAEGYRRLEAEDLAAPVAISLHATCCTIAPGEALRLSIAAAAFPAYPVNPGTGA
                     ASIDTPRHAARIITLGLATGAGTGSVLRLGAGL"
     gene            complement(16327..17670)
                     /locus_tag="VFF98_09755"
     CDS             complement(16327..17670)
                     /locus_tag="VFF98_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU63955.1"
                     /translation="MALSAFLERLGDVPISRDPAEIRRKSKDYFWFSPVLAEELQHLE
                     AEVIVTPRDRADLLRVAAASAASGVPLTARGGGTGNYGQCVPLAGGAVVDLTGLARIR
                     RLEAGAVEVEAGIAMFDLETRLRAEGQELRMFPTTWHTATLGGFVAGGTGGVGSVHWG
                     GLREPGNLLAAEVITVEETPRVLTLKGQQCNLVNRTFGTTGLISALTIPTRAAVDWQD
                     MLVTFAELADAIAFGQALCRAQGVEKRLVSLSCGALAGYFRPFAGQIPAGHAVVAVMV
                     APAGCLAVEELAAAHRGAVALQRANAAAEADPDSLPIYEIAFNHATQHVLRRARGYTY
                     LQSLCPSAEVLLALRARLGDEVMWHVEFIGFDGGHAMNTLPVLRWGGRQRLAEVIALF
                     EAEGAPVANPHVVTVEDGSRYKRLPGDQLGFKQAVDPAGLLNPGKMRSFKRADAA"
     gene            complement(17678..18499)
                     /locus_tag="VFF98_09760"
     CDS             complement(17678..18499)
                     /locus_tag="VFF98_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZU63956.1"
                     /translation="MIPARHWQDMPSSAFGQLAANTVAILPVGAIEQHGPHLPVFVDS
                     CINAGLLDIALKTAPADLPITALPMQSVGKSDEHISFPGTLTVSAEVLTGLLMEIGRS
                     VFRAGIRRLILLNSHGGQPQVMDIVARGLRVELGMFVVNAAWSKMGMPKGLFSPEECE
                     HGIHGGEIETSLMLHLRPDLVDMGKAENFVSLSQTMQERFRILTPEGKVGFGWQVEDL
                     NPKGACGNAAAADAERGRLVAEHAASRFLDLCAEVADYPAETIGVPARAASVQQG"
     gene            complement(18531..19502)
                     /locus_tag="VFF98_09765"
     CDS             complement(18531..19502)
                     /locus_tag="VFF98_09765"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZU63957.1"
                     /translation="MEIYAPPQEADSLPVIDISGPETEVAAQIRAACLDTGFFYAAGH
                     GIAPELVAGQFAAARRFFAVPAAEKAALDMRHSALVCGYEALCGQVLDSQDGGTEAPP
                     DLKEAYYCCTNLADDNPSARSPARVVGSNQWPAGLAAEVAHTRAYGEAVQALGDRLLE
                     LLALSLDLPRAWFSPFFAGSPAKLRFIRYPPQAADAAFNQIGAGAHTDWGGITVLAQD
                     ELGGLEVRDRQGRWIKATPVEGTFVINLGDLMARWTNGVYASNMHRVLNNAAARDRYS
                     MPFFYSPHDEATIAPIASCISVDNPPRFDVCSAGEHMREMFRRSYGY"
     gene            complement(19518..21755)
                     /locus_tag="VFF98_09770"
     CDS             complement(19518..21755)
                     /locus_tag="VFF98_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU63958.1"
                     /translation="MALAIGLATTPAHAADGATDSADAASADTASAEGGSGIVVTAQR
                     RSQSALEVPMAVTAISAQRLTEERVERVGELIQATSNVEVREIRPGGGQPAITIRGVG
                     MNDFTVASNPSTSVYMDGVYSPNIGTISQQVFDLAEVEVLKGPQSTLYGRNATAGAVI
                     INSAQPTDHLSGYGTVSYGNYDAVNVEGAISGPITSNLTGRLSAKTRQMYDGWMNNTY
                     PGGQKIGTLHQTAVRAQLKWQPTDRLSFQGIFGYQYENDVPGAMTVFGRKTTGGSSSS
                     PSSTYCATSNANQIDFGGNCASSWGATRGSTNLLTLSENDSWLVHSNTYTGTLISSYK
                     GDGFTVKATTGYLWWHEYYNKGDGLPVTEQHYLLDQKTWQISQDVVVSSTGQHFIDWM
                     AGAYASSGNVNNPTYSYTPINGYNLFNTNNTYTQTLQGFAQLDTHITPKLTLGTGARF
                     IYEHDHKAGGTFKYSADWSTYMATQAYLDQSIQQNAISWKANLDYKPDANTLVYGSVT
                     EGFKSGGFAASPNATSSAQLNPYKGEHIYAFEVGSKASLAHHKVDLEGALFYYVYHNM
                     QTNQQQLVGNLNVNVFGNLPKAHLKGLEVSATFRPISGLELKLDGGWVDTWVGAFTSG
                     GVLYKAGNRFANAPSFAGTVSGRYQWHLTDAVDLALGASAHRQSSVYANTENTAIYRV
                     NTAATLVNAQFQVLLPHKGWSMMFWGKNLTNCEYTNSTYQNSSTVNTLYNMPRTYGVS
                     LTKNF"
     gene            complement(22143..22898)
                     /locus_tag="VFF98_09775"
     CDS             complement(22143..22898)
                     /locus_tag="VFF98_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU63959.1"
                     /translation="MATMALAGWRSMAERLAGRLAVVTGAAHGIGAGVAQLFRAEGAT
                     VIGIDREAGAGVDIACDLVDEAAVAATFARIGAEHGRIDALVNAAAFATFAWIEEMTY
                     AQWQRTLVGELDIVFLPTRAAWPHLKASGRASIVNFASANARHALPGSPALAHCAGKG
                     GVLAMTRQLAMEGAPFGIRANTISPGFTLTAATRAHLARDPALEQHVLAGNMLKFFGE
                     PEDIGWGAVWLASDEARYVTGADIPIDAGATAW"
     gene            complement(22858..24111)
                     /locus_tag="VFF98_09780"
     CDS             complement(22858..24111)
                     /locus_tag="VFF98_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63960.1"
                     /translation="MIRHHQPGWMTGVALLLPITLSTMAIVLLAPVLPGILKEFSGVP
                     GFEYWVPMILTIPALCVALFSTLAGAAGDYFGRRRLLMASFVAYAVVGLAPVFLHDLT
                     AILVSRVGVGLAEALIMVLSTTMIGDYFHGPARDKWLAGQTAFASMSALVFFNVGGQL
                     GGFGWRTPFWVYASALVMLALVARFTWEPADDLGDEAEAPPHNASWAGFPWGRMAVIL
                     AITLYGAVFFYTVQIQAGLGLTLLGVLDPARVGFLTSVASVGVPLGTLIYSRVGRWPV
                     QRLLLIEFALLAVGFLLMARAGTPNGFLVGCFLNQLGAGMLLPTLLVWAMSLLSFEVR
                     GRGAGMWQSAFAFGQFLSPVVVTFAAQHAGGLQAAFGVLSAGAVLGLVVVLAVAGRIG
                     RAADEPDGNGNGNGHNGSGRVALHG"
     gene            complement(24287..24748)
                     /locus_tag="VFF98_09785"
     CDS             complement(24287..24748)
                     /locus_tag="VFF98_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZU63961.1"
                     /translation="MPDHSHIDQIDRRIVEQLRRDGRMTITELAARVGLSKTPCQLRL
                     KRLVEEGYIRGFRAVIDPIKLGVGHIAFAEVRLSDTREKALEEFRRAVLQVEEVEECH
                     MIAANFDYLLKVRTRDIHSYRKVLGEKISSLPHVASTSTFVVMETLVDDGA"
     gene            24884..>25258
                     /locus_tag="VFF98_09790"
     CDS             24884..>25258
                     /locus_tag="VFF98_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63962.1"
                     /translation="MTDTPAAPFARFAPPIAAAAPLRTAITAAPLRAAITAACRRPEP
                     EAMAPLIAAARLPEGTARAIAATACTLITALRQRGAGGGVAALVKEYALSSQEGVALM
                     CLAEALLRIPDAATRDALIRDKI"
BASE COUNT         4133 a         8663 c         8494 g         3968 t
ORIGIN      
        1 cggtgtagtg gctggtgaac cagccgatgg ccagcgcgcc cagcgcgcct gcggccagcg
       61 cggccagcgc ctgcgccgcc ggcaagggcg ccaccgctac cggccccgcc gcttcccccg
      121 cctgcgccgg cgcgcgcgcc cattcgcgcc agccatagag gatggccagg ccaaacaacg
      181 cctgcagcac catatcggca tagagccgca catcgaggaa cagcttgaaa taaagcgcgc
      241 aggccagcac gttgagcggc cagcacagca tgtggcggcg cgccgtcagc cagatcgcca
      301 gcgcggttac ggccaccgcc accatttcca gcccgctcat gtcgcagcgc cccttgtggc
      361 ggccagcagc gcctgcccgc ccgccccggc aaaccagcgt gcatggccca gaagataggt
      421 atgccaggcc agatcggtat cggcccgcag ggccaggatg ccctcgaaat agaccatttc
      481 ggacaaggca aattccacat gcaccagcgg cagcaggcgg gcgagcaggg cccggtcctc
      541 cccggtcagc ggcgcggcgc tggcatagcc ggccagcagc gcatcggcgc aaggcagatc
      601 ggccagcgcc gcatcctcgc ccttgcccag ctgcagccag gccaccacgc tgcgctccag
      661 cgcgatggcg aggtcaaaca gcgcgcaggt gcgatcggcc aggccaaaat ccatgatgcc
      721 ggccacgccc cccgccccat cccacaggag gttggagggg tgccagtcgc catggcccca
      781 aagggtgggc cgctggccca gcgcgcggga gaggccagcg gcatcggccg cggccaacag
      841 cgcggccagt tccgcccgcc agccagtgcc ggcaaaatag gcgcccagcg ccggccagcg
      901 cgccacgaag gcttccgccg ccgcctgtgg atcgccgccg gcaaacagcg tgttgcccgc
      961 taccagcggc gccggcgcgc gagcgggggc ggcaaaaccg gcggcggcgc ggtgcagccg
     1021 gcccagcatg gcgcccgccg catgggcatg ggccgggcag aggaagccgg tccacgaggg
     1081 gcgctcgcgg taaagatcct cgcccgccga tttgcggtgc acttcccagc tccacccgcc
     1141 cagcgcaaag gcgctggcgc cggccgcatc ggccagcaca tcggccaccg gcaggcccgc
     1201 ctggcgcaga tgggcgatga aggcatgttc ctcggcaagg ccggcggggc tgcgcagggc
     1261 ggggctgtgg cgcttcagga acagctcgcc ctggctggtg cgggccagcg cggcggcgga
     1321 aaacgggcgc ggcgagtgcc attccagcgc ctccagcacg ccggcgcccg gcacccgcgc
     1381 cagcacggcg gccgcctcgg caggcgtgac cggcggccag gcgggcgcgg ccagcccggt
     1441 gcccagccca tgcaccagat gcccttcggc caccggcccg ccctagaact ggcgcgagag
     1501 gctggcgacc accgccgccc ccccgccgat gtagtaagtg ggcgcgctgc cggcaatcac
     1561 cgtgccgttc agcccggtgg tatccctggc attggtgctg agcccggcca cgcccgacag
     1621 cacatgcgga ttggtgacat tgaggaaatt gagcctgaga tcggtatgcg gcgcctcgcc
     1681 ggtgccatgg ccggccagat gcacgccgac agacagatcc agcgtggcat aggcggcaat
     1741 atgctcgtcg ttcatgaagg tggcatattg cttgcccaca tatttgagcg cgaagctgcc
     1801 gaacagccgg cccctgtcat aggtgccgcc cagcgctgcc tgcaccgccg ggctggagac
     1861 cgcctgcttg ccgcgcgtgg gcaggtaatc gccatccacc ggcaggttgt cgtccagccg
     1921 ggcatgcagc cattcgcccg aggcatagaa gctgaggccg gcaatggggc gccagtcgat
     1981 ctcgccatcc aggccatagc tggtctgcgc gccgccgttc atcgtggtgt tgatcagcgc
     2041 gccatcgaca tcggccagcg ttgccagctg gcggttgcgg aagtgatagt ggaaagccgt
     2101 gaggctggcc gacagcaccg cgcctgtata acgccagccc agttcctcgg agatcgcata
     2161 ttcgttcttc agcgccgtgg tgccgcgctg ggtcacatcg ccatagccgt catagccgtc
     2221 gtagagcgta tattcgtcgg gcgcgcggaa atgggtggag aggttggcga agaactgatg
     2281 cgcatcgctg gcccgccagc tgagcgcggc gcgcggcagg gcggcgaaac tgtccgtgtg
     2341 caccgcgctc tggtcgcccg gcagataatt atggccattg tgcagcacat ccacgccctt
     2401 gaagccgata tcggcgccaa gccggccggt gagcgcgatg tgatcggcga ggaagaagcc
     2461 cttcaccacg gtggtggtgc ggatgttctc gatcgccagc aggcggccat cggcggtgtg
     2521 gatcgcattg gcgtcatagc cccatgcgtc cagcggattg ccattgccat caagggctgt
     2581 gaaagattcg acatcgcggt cggtaccata atcgaaccac aggccggcgg tgaggcgatg
     2641 ggcgcccagc gtcactgtta atttcgacac atcgccggtg cgcagctggt cgcccagcca
     2701 gttgcccagc acggtggcgc tgctggcccc gcccggcagg ctgatggcgc ccagcgcttc
     2761 tgtgcccaga taattgccgg tgctatccag ctgggtgcca tagggcgaat tgccgtagcc
     2821 gcgctggaaa tagaaagtgc tgtcgaaagc cgcgccctcg cccagttgca gatgcagcgg
     2881 ggcagacaga taggtgttgc ggaagggctg gcgatagagc cgccagtaag ccgtgtcgcc
     2941 ggcggtgtag gtggcatcgt agttatagcc gcggccatag gcctgccaat cggccagcgt
     3001 ggggttggga taggtggaat tgcgggcatc gttgaaggaa acggcgatgg cagcgctgtt
     3061 gccctcgccc cagtgcttgg tgatcttgcc atcgatatgg cggcgcacat catagccggc
     3121 gccgcgccag ttatcggcat aggtgttcga ataggagacg aaggcggcaa tgccgctgtt
     3181 accgatctcg ccggtatcca gccgcacgaa gaagcggcgc atgttgtagg aaccggccga
     3241 gatggtggcc gccagcccca tgcgcctgtg cggatcatca aggctgagcg acagcagccc
     3301 gcccgccccg ttcaccaccg gcgcatccag atccacagcg cccggcgcca gcgccacgct
     3361 gcgcaaattc tccgcatcgg caaattgcga gggataggca tcgtaatagc cgatgtcgtt
     3421 ctgcggcgcg ccttccatca gcacgccgat ctcgtcctgc gtcaggccgc gcagggtgag
     3481 gctgttgctg gtggacaggc catagggatc gctcatcgcc acattggcgc cgggcagcag
     3541 gttgaccagc tggaaggcat tgagcgtggg cgcctgcttg gcgatgaagg cgctgccaat
     3601 ggccgagacg gcaaccggca ccgtttgcgc cggcagcagc ccttcggggt cggggtggcc
     3661 ttccacgcgg atgggggtgt gatcggtgcc ctcctcgccc tcggccagcg ccgcgcccgg
     3721 caggcccaag cccatcagcg ccagcagcgc ggtggcctgc agcaagtggc ggcgcaaatg
     3781 gcggcccgtg gccgatggct tgcccgacat ggcgatggtt ggctggcccg acatggcttt
     3841 ttcatccctg attgcaatcg gccctgtggc cgacgcggca ataagaggac cccggccgcg
     3901 tcgctgtggc cgtggggcgc cccttaggcc ggcaaggcgc cccaaggcaa gagcccggcc
     3961 ggatcagcag gcggcgggcg ctgcggcctg atgcgcgcga taggcattgg cggcgcggcg
     4021 gatgtggttg gcggcaatgg cggcggccca ttcgccatca tgggcggcaa aggcgcggat
     4081 aaggtccaga tggtcggcct ggctgcgcac catgtcctcg tgggtatagc gctcggcggt
     4141 gcgatggacg atcacctggc tgaccaccag ccgcatcagc tgggccagcc ggtcagaccg
     4201 ggcggcggcc atcatagcgg cgtgaaacag ccggttgccc tcgacaaagg ccggcacatc
     4261 aatggcctgc ggatcggcaa tggcggcatc gatgcgggcg gcatggccgc gcagttcctc
     4321 gatctcggcc ggcgtgatga ggctggcggt gcggcgcgcg caatgggcct ccagccccgc
     4381 gcgcagggca aacagctcct cgatctcgtc gtccgaccag ctgggcacga acaggcgctt
     4441 ggtatcggtg cgttgcagca gcaattcgct gaccagccgg gcaatcgcct cgcgcacggg
     4501 ggtgcgcgac atgccgcaca gctgggcaaa ttcctcctcg ggcacgaact ggcccggctc
     4561 gaaacggccg gcgagaatgg cgctgcggat ctgccggtag gcctgcatgg ccgaggtgga
     4621 ggccgcgcgt tcggccccgg cccgcgccgg ttcctgccgc gcggacatgt ctgaagattg
     4681 cgccaaaccc tgccctttgt gctggcgcgg ccgatcggcc gggccgggtg caaactagcc
     4741 gcgcgcgcgc cggcttgtcg agagccgaaa ggcgcgcggg gtgggcggcc tagggcgccg
     4801 gggtgggcgc tggcgtgggc gccggagaag gcgctggcgt gggcgccggc gttgctggcg
     4861 caagcgcagg ggccgccgga gccggagccg gagccggagc cggagccgga gccggagccg
     4921 gagccgcagg ggcggatggc ttgtgcttgt gggtgtgcaa ggcggcaatc tcggcctcgc
     4981 gctcgcgcag atagatggcg cgtttggcgc gataggggtt gggggccttt tcccagtcgc
     5041 gcccgccatt gcggtcctgc gtcatgcggt cgtccagccc gttgacgacg gtgatcgtcg
     5101 catccaccca gatggtgttg agcgccccgc cgatgatcgt gggcagcatc agccgatcag
     5161 ccgtggtgcc caccagatcg cgcagggtgg tggaacccat cagcggcacg aacatgtaag
     5221 ggcccggctt cacgccataa aagcccatcg tgtcggccag gccattctcg cggtagggca
     5281 ggcgaaaggg cttgcgccgg gcgatatcga acaggccgcc cagccccagc gtggaattga
     5341 gcaccagccg gcccagcgtc tcgccggtct tgccgatgcg gtgctgcatc aggaaggcca
     5401 cggcaatcgc cggctcgcgg gcgttgtaga gcacgttgtg gaaaccatcg cgcaagggtt
     5461 tgggcacccc gcgcttgtag agccggcccg ccgggccaat gaaggcgcga tcggtggcct
     5521 gcaccgcctg gaaggaggtt tcgttcagct tgcgggcggg atcctgccgg tctgcccagg
     5581 tcttgccgct gacgacgatg ggcttgggcg cccctgcgac agccggttcg gccggggcgg
     5641 caggcggaag cgccgggctg gccgttaccg ggctggtggc aggcgccggg ctggcggcgg
     5701 gcgccgggct ggccggttcg gtggtctggg cgggggtggg ctcggtggcc tgggccagcg
     5761 aggcgctgcc cagcagcggc agcgccatca gcgcgcctgc ggcccaggcc ggcatccgtc
     5821 gcacaggggt tgcatcatca tccagtcggt ggccggccgc ttcgtgtgag ggtactgtca
     5881 tcctgccttc ttctcatggc ttgtgtcgca cagccgggtg cccggccggc tcctgcacca
     5941 accgcacgcc cggggcatgg cacccgcgct gcccccccgg ccatcgcgcg cggccaccat
     6001 gccgaactgc cgttccccct ttgcagcata atttcacaaa ccatagtcaa aactggatga
     6061 gccggcgcaa aaatcctgcg gccggctcaa tggccacacc tgcgcgccgg ccgcgttcag
     6121 gcggccgcca cttcctcctc gccatgggcg gccagcagcg ccagcaggcg cttgcgcatg
     6181 atgaggccgg gatgatcgga tggcatgtgc atctcggcct tgcgggccag atcgagcacg
     6241 gcatcggggt cggtcgattc cagcatggtg cggtcttcca gcagcacttc ggcatcccag
     6301 tcgatcagct cctgctcggg gcaatcggct tcggtgtcgt tgcggaacaa cagctgggtc
     6361 acctggatgg cgccatcgtc gatcggcgtg gcgcagttga agatgatgtg gcgccggcca
     6421 gacggatagg tgatgtcgag ccggcggcag aagggcatgt accaggcgtt gcgcatggtg
     6481 cgcaccgtcc attcgccggt atcgcccgtt acccggtggg cgcggggcgg attggccacg
     6541 cgcacctcgg tttccgccca gaagccctcg tcggtctcct cgatggcata gcgggtgggg
     6601 cgcggccggt cgatctcgcc gaaggtgccc ttgtggacga aggcgatatg ggcctgatcg
     6661 aagctgtttt ccatcagccg cagtgcgctg cattcccagc ggccataatg ctggtagatg
     6721 cggcgaaagg cggggtcctc gtcctcgggc acggcgggga tatcggccag cggctgctcc
     6781 agcgccaccc acacatagcc atagcgggcc tgggcgtgga acttgcggat ctgcgcattg
     6841 ggcagcgcct ggccctcggt gaattgcgga atgcgggcca ggtggccctc gctgtcaaat
     6901 tcccagccgt gatagccaca gatcacccgc cccttgtcgc accagcccag cgacaggcgc
     6961 gcggtgcggt gcgggcagcg atcctccaaa gcggccggct ggccatcggc gcccagaaac
     7021 agcacgatcc tctcgcccag cagggtgaaa gggcgcggcc ccgcggccag atcgtccagc
     7081 cgcacggtgg cataccagaa gcggcgcagc acattctgtt tggtggcaag catggtcggt
     7141 tccctgaatg cggctcggcc cctgtgccgc cgcgcatgca ctcttgtgca ttgcggcaag
     7201 actatcgcta tgcgcgctgc aaaaaagcgc cgaattgcgg atagggtgat gccttttgag
     7261 caccaaagcg cgagcaaagg cggatgctgc ggcgcacaac atatcatcaa aaaggcaacg
     7321 ccacgtgcca ttaaaagtgc ttttcaggca tcaccgctca tgatcatctg gcgcttcctc
     7381 cacccccaag ggaacctcca cttcagccca tgcgtatcaa ggaagcctgt ctggttctgc
     7441 tggcgatggc agcgctggct gtggcgctca gcctgtggct ggcgcgcggt ggccccgcgc
     7501 cctcggtcca caagcgcttc gtgctgcagc tgagctggcg cgcccagccg gaagtgggcg
     7561 gctatgtgga agcgggggcg cgcggctatt atcaggcctg cgggctggat gtggagctgc
     7621 gccagggcgg cgccggcatc gatccggcgc agctgctggt ggcgggcgct gtggatgcag
     7681 cgctgctgcc ccaggccgat ggcatcatcc acatgaacgc cgccggcttt cccgcccgcg
     7741 cgctgtttgc cgccatgcag cattcggcga tggcgctgga tgtgcacgcc gacagcccga
     7801 tccgcgccat ccccgacatg gtgggccacc cgatcatgct ctcggccagc tcgcgggcca
     7861 gctggtggcc gttcctcaag cggcgcttcg gctttgccga tgcccagctg cgcaatttca
     7921 ccgggcaatt cgcccccttc gtcgccgatc ccgatgcggt gacgcaggac atgatcagca
     7981 acggccccta tcagctggaa accaagagcc atctgcaggt gcgcaccttc agcctcgcgc
     8041 cctggggcta tgatccctat ggcgccatcc tcaccgtctc gcagcagacg atcacgcggg
     8101 atccggcggc catgcgctgc ctcgtctcgg cctcgcagcg cggctggcaa agctatctgg
     8161 ccgatcctgg gcatgcctat ggcgcgatcc acgcgatgtc gcccgaatat accgacgggg
     8221 ccagccgcta tgcctacaag gtgctgatgg aaaaccacat cttctccacg cccgagaccg
     8281 accggcaggg cctggggatc atgagcgcgg cgcgctggcg ggcccattat gcgctgctgc
     8341 gcgatctggg cgtggcgccg gcgggcctcg atcccgacag cctcttcacc aacgccttcc
     8401 tgcccggcgc cgcccctgcg ccccactcct gacccaggac ccgagccgat gaacacgccc
     8461 ccgctttcca cctgccccta tccgatcgcc gagatcagcg cggcgctggg cgatatcgag
     8521 catacgaccg acccgatcct gatccgccgc aagagccagg accgcaacgc cgtgagcccc
     8581 ctgctgcgcc agatgatggc cgacaagcgc gccgacatga tcgtcacccc caaaacgctg
     8641 gaggagatcc gccgcaccgt gcaggccgcc gtgcgccacc aaacgccgat caccgtgcgc
     8701 gccgggggca cggccaatta tggccagtcg gtgccgctga agggcggcat cgtgctcgac
     8761 atgaccgcct atgccggcat cacccggctg gagccgggcc tgctgcgcgc cagatccggc
     8821 acgctcactg ccgatatcga cacagaagcg cgcaagatcg gctgggaaat gcgctttccc
     8881 cccaccacca agcagggcgc cacgattggc ggccatgtgg cgggcggaca gggcgggccg
     8941 ggctcggttg tctatggcac ctggcgcgat cgcggcaatc tggtgggcgc cagcgtgctg
     9001 acggtggagg aagagcccag ggtgatcgag ctgaccgggc gcgatgccca gttcatccac
     9061 cacacctatg gtgccaccgg catcatcctc gagatcgaga tcccgctggc ccccgccttc
     9121 ccgtggatcg aggcggtggt ggccttcccg gactatatgc aggcggtgcg ttttggcatc
     9181 gatctggcca atgcggcggg catcatccgc aaggtggttt cggtgcaggg ctggcccacg
     9241 ccctggctgt tcaagccctt ccgcgccttc gtgccggaag gccatgccat cgtctccacc
     9301 atgatcggcc agcagacatg ggcggatttt gcccagatgg tggcagagat gggcgggcag
     9361 atcgtcgccg aggcgcccga ggggcagggc ccctacaagg cgccgctgac cgaattcgtc
     9421 ttcggccacg cgctggtgca gatccagaag accgagccca aacgcgccgt gctggaaggt
     9481 ttcttccgcg atacggccga tctggccggg ctgatcgagc gggtgtacca caaggttggc
     9541 caccacggcc cgctgcgggt ggagctgatc cgcatctcgg gcgagctggt gggcacgggc
     9601 gccccctatt tcctttatga aagccccgag cagatggccg ggatcgtgcg cgagatgcag
     9661 gaggccggcg cgctcgtctc caattcgcac acttccaacg tgcgggcggt gggcaagaag
     9721 gagatcaccg cgcgtgatct cgccttcaag cgcgcggtcg atccccatgg cctgctcaac
     9781 cccggccgct tcgaggtgga ggaaagcgcc gatgcgagct ttgccctggc tctgcccacc
     9841 gacagctggg accgccggct gggctgatgg acagcgccgc ccccttcccc ctgccgccgc
     9901 tgcgcgcgcc cctgccggtg cgcgaggtgg aagtctgcac gcagttgctg gccgatggca
     9961 cccggctcga tgccgatgtc tatcggccgg atgcacccgg cgattatccc atgctgctgc
    10021 aacggcaggc ctatgggcgg cggatcggtt gcaccctctg ctatgcccat ccggcgtggt
    10081 atgccgcgca gggctatgtg gtggtggtgc aggatgtgcg cggacgcggc agctcaaccg
    10141 gcgtgttcca ccccggcctg catgaggccg cagatggcgc cgccgccgtg gaatgggccg
    10201 cccggctgcc cggcgccaat ggccgggtgg gcagtttcgg cttttcctat caggcctata
    10261 accagctgct ggcggccacg ggcaattgcc cctcgctggt ggccatggtg ccggcgatgg
    10321 ggccatggga tgccgcccgc cactggatgt tcgagaatgg cgcgctgcgg ctgaagcagg
    10381 cggtgggctg gggcgtgcag atcaccgccg agggcgcccg ccgcgtcggc gatgccgccg
    10441 cctatgccga gctggccgcc gccggcctgc cggtggaggg ccccgtttcg gcccggcccg
    10501 atgtgctgat gcgccacgcc ggcctctccc actttgccga atggatcggg cggggggcag
    10561 atgatccggc ctgggcggcc atttcgcccg ccgcccaagc ccaggcgctg gccgcccgcg
    10621 ccctgcccac cctcctcatc ggcggctggt tcgacacgca tctggccagc acgctggccg
    10681 ccgcccgggc gctggcgggg cctgccacgc ggctgctggt ggggccctgg gcgcatttcc
    10741 cctgggggcg caagaccggc cagatcgatt tcggccctgc tgccgcgctt gacaccgacc
    10801 gcttgcagat cgcctggttc aaccgctggc tgaaagagga ggacaacggc attgacgccc
    10861 tgcccccctt gcgcctgttc gatatgggcc aacctgcagg ccagacccgc tggcgcgatt
    10921 ttgccaccat gccgccggcc actgtgccgc ttttcacaag cggcgatggc cacgccgcca
    10981 tccgccccga ggccggccag ttgcagcccc agccccccgc cccgatggcc gaaaggctgg
    11041 tgcacgaccc ctggcgcccg gcgccggcgg tgggcggatc gcatggcacg ccgccgggcc
    11101 cggtggaccg cgccggggtg gaaagccgcg gcgatgtgct cactttcaca agccctgcgg
    11161 ccgacaaggc ctggctgctg gccggcgaga tcgccgccgc cctcaccctt ggcgccgacc
    11221 agcccggctt cgatgttgcc tgcacgctgt cccgcgtcga tggccagggg cgcagcttcc
    11281 agctggcggc cggctatatg cgggtgccgg caggcgcggg cctgcgcgaa atccgcctgc
    11341 cgctgggcgc cacctgcgcc acgctgcagg cgggcgaggc cttgcgcctg tcgatcgccg
    11401 gggcagcctt ccccgccttt ccggtgaacc cgggcagcgg cgccgatccg gccgcaaccc
    11461 cgcgcgccga ggcccgcatc atcaccctca ccctccattg cgggggcgcg gcgcccagcc
    11521 tgctgctgct gccggagact gccgcatgac cccgcccaaa gccaccgatg cgctccacga
    11581 ggcccagatg ctttcttctc cgccagagcc tgccgctgaa gcccccgccc ccgcccctgc
    11641 gccccccgcc cgcaaacgca ccgatatggc agaccgctgg ctgccgctgg ccggcggcct
    11701 cggctttctg gcgctgtggc aggtggcggt gaaggtgttg gccatttccc ccttcaccct
    11761 gccgccgcca tcggcggtgt tcgtggcgct gtggcaggat gggccggagc tgctgcatgc
    11821 cctggccttc accgcggcga tcaccctttc ggccttcctg ctggcgctgg cgagcggggt
    11881 gatgctgggc gtggcgctca cccagaaccg gcggatagag cgcatgctgt ggccctatgc
    11941 ggtggcgctg caggtcacgc cgatggtggc gatcgcgccg ctggtggtga tctgggtggg
    12001 gctgagccgg gcctggctgg cgctgctggt gctggcctgg gtggtggcct tcttccccat
    12061 cctgtcgaac acggtgctgg ggatgaaatc ggccgatcac gggctgaaaa acgtcttcac
    12121 cctctatcgc gccagccgct ggcagcgttt ctgccacctg caactgccgg cggccctgcc
    12181 ctatatcatc gccggcatgc gggtgtcggc gggcctgtcg gtgattggcg ctgtggtggc
    12241 ggaatttgtc gccggctctg gcggcaccac cggccttgcc tgggtcatca cccaaagcgg
    12301 gtcgatgctc gacattgccc gcatgtttgc cgcccttgtc gtcctcaccg cctttggcct
    12361 ggccatgaac atggccaccg gcctgatcca gcgcctgctt ctgggcgcct ggcacgaaag
    12421 cgccatcgag caggagaact gagctatgca gccccatccc caaggcagcc cgccgccacc
    12481 gctggtgcgc ctggcccatg tcggcaaggt gtatgacagc ggcaccatcg cccttgccga
    12541 tctcacgctc gatgtggcgc cgggcgagtt catctcgctg ctgggcccat cgggctgcgg
    12601 caaatccacc gcgctgcgga tgatcgccgg gctggggcgc cattcctccg gcctgatcga
    12661 ctggccgacc agcgcctatg acgcggcggg cgagcccgag cgcgagatcg gctttgtctt
    12721 ccaggagccg cagctgatgc cctgggccac cattgccgaa aacgtgcgcc tgccgctcaa
    12781 gctcgcccgg gtgccgcgcg cccgggccca ggcgcgggtg gccgaaacgc tggagatggt
    12841 gggcctttcc gcctttgccg atgcctatcc gcgccagctt tccggcggca tgaagatgcg
    12901 cgcctcgatc gcccgcgcgc tggtgacaga gccgcgcctg ctgctgatgg acgagccctt
    12961 cgccgcgctc gacgagatca cccgcacgcg cctttccaac gatctgctgg agatctggca
    13021 gcgcaccggc tgcacggtgc tgttcgtcac tcacagcgtc tatgaaagcg tgttcctctc
    13081 cacccgcatt ctggtgatgg cgccgcgccc gggccgggtg atcggcgagg tggcgatcga
    13141 tcatcccggc ccgcgcgata ccgcctggcg ccagtcgccc gcctatcagg ccggctgccg
    13201 gcgggtctcg gcgctgctgg cagaggcgat gggccagccg gcgccggcat gaacggcccg
    13261 ttccagcgcg ggcctgtaca gcacgcccct gcccgcctag gctgagcccc atgctgcacg
    13321 cacgtttgct gacctatatc gacgaggtcg ttcgccaggg ctccatccgg cgggcggcgg
    13381 aaaagctcaa tgtcgcttcc tcggcgatca gccggcagat cctggcgctg gaagagcaga
    13441 tcggcgcacc gctgttcgac cgttcgggcc ggcgcctcag gcccaccgcc gccggcgaac
    13501 tgctggtgcg ccatgtgcgc gagacgctga aggagatgac ccgcacccag gcgatgatcg
    13561 aggagctgaa gggcctgcgc cgcggctctg tctccatcgg cctgatgagc gggctggcgg
    13621 ccaacatcct gccccgcgcg gtgaatgatt tccacgagag caacccgcgc gtggaagtgc
    13681 gcctgaagct gatgaccacc ggcgacgaga tcctcaatgc ggtggaaaag ggcgaggtgg
    13741 cgctggggct gggcttcgat tttccgcgcc atgcgggcgt gcgggtggtg catgcggcca
    13801 ccggccggct gggcgcggtg atgaacccgg aacatccgct ggccggccag agcgagctgc
    13861 gcctgcgcga ttgcgcgccc tatccgctgg tgctggccga ccccaccacc gccatccgcc
    13921 cgcatatcga cgaggccttt gcccgttcag gcttcaccgc gctggccttc accgagacca
    13981 attccatcga gatgatgcgc cagatggcga tcctgggccg ctcgatcact ttcctcacgc
    14041 ctttcgacat cgagttcgag cagcgcatgg gccgcctcac ctatgtgccg atgccggaac
    14101 tggcgcgcca cgaacagcgc ctgatgctgg tgcagaacga gcggcgatcg gatgcgctgg
    14161 caggcctgct ggccgagcgg ctgaaggcgc tgatccattc cgttgccgaa acggggggct
    14221 agaggcaccg ccgcgccgaa accgggggct agggcccccg ccccctctca gcctttggtc
    14281 aacaccgcca gatcctcggc atccagcgcc accggctcgc cggcgcggat cagctcggca
    14341 aagccctcgt tgggcaccat gaaggtcagc gtgtagagcc tggtggcgcc ggtgttctcg
    14401 atcacatgtt ccgcccccgg gcgcagcatc agcgcatcgc ccttggcgat ggcgcggctg
    14461 gcgccggcgc aatgggccat gccctggcca tgcagcacat agaaaaattc atgggcgatg
    14521 gcgtggctgt tgggcggcgt gcgcccgccc acgttgaaga tctccaccac gcagaccggc
    14581 tctatgccat cgccctcggg gtcgaacagc agggcgaaat agttggtatc cttgggcgaa
    14641 atgcgaaagg ccgccacatc ggcaacgctg cgggcaacaa aatgatccat cggcaatgtc
    14701 cttttcacag gcccgcgccc aggcgcagca cagatcccgt gcccgcgccc gtggccaggc
    14761 ccagggtgat gatccgcgcc gcgtggcggg gtgtgtcgat agaagcagcg ccggtgcccg
    14821 ggttcaccgg ataggccggg aaagcggccg cggcgatgga aaggcgcaag gcctcgcccg
    14881 gcgcgatggt gcagcaggtg gcgtggagcg aaattgccac cggtgctgcc agatcctccg
    14941 cttccagccg ccgatagccc tcggcgatct gccacacgcg cccctcggca tcgatgcgcg
    15001 agagggtgca ggcgatatcg caggagggcg catcggccag cagcgacagg ctggccgata
    15061 ccgcgccggc aatcgccagc ggggcggcca ttggcgctgt ggtaaaagtc agcacatcgg
    15121 cgcgctggtc gtggcgggcg cggtccacag gaccagccgg agtggcaaag gcgccgccgc
    15181 aggccggcgc cggccgccag ggatcatgca ccagataatc caccgccggt gcggccggat
    15241 ctgccagcaa atgcccgctt tgcgtggcaa cggaaacgcg cccgtcgccc gccagatgca
    15301 gcaccacctc gcccgccgcc agttcatggc accagcccag cgtgcccatg tcaaaccatt
    15361 gcaacggggc gggcggcgcc ccctcgccgg ccagccagta gcggaagaag gccacctgcg
    15421 cggcatcgat cggatgcgcc gcctcggggc cgaaatcgcg ctcgcccacc agccggtccc
    15481 acgggaaatg ggtccacggg ccgacgatca gcgtgccgcc cagcgccttg tgggcggcca
    15541 gcgtgccgat cagatgcgta tcgaaccagc cgccgacgaa cagcgccgga atgccggccg
    15601 cggcaatcgc cggctcttgc gcggcgggcg acaggccggc ccagaaggga tcgtcggccg
    15661 gcgtgtcgcg ccaggcggga taatggccca gcgccagttc tgtggccagc gcttcgggcc
    15721 ggcccggcac cgcgccggcc agcggcaggc cgcgcgcggc gctggccagc ttttcaaacg
    15781 cggccacctc gccctggtgc agcgccgaga gcgccgccat ctgcacgccc cagcccaggt
    15841 tgggggccag ccgcatggcg ccctgctcgc tcacccaatc cgccgtcaga tcccagccga
    15901 ccatcgccgg cgccatcgcc ttcagcgccg gcgggcggtg agcggcggcc agcaattgca
    15961 gcgtgccctg ataggaaaag ccgaaaagcc cgacatggcc gttcgatccg gcaaggccgg
    16021 cggcccattc cactgcctcg gccccatcag cgccttcgtg gcggaaggca tcgaacacgc
    16081 cttccgaggt gccgcgcccg cgcacatcct gcaccaccac catgaagccc tgcccggcat
    16141 accaggccgg atgggcatag cacagcgaag agcccagccg ccgcccatag gcctggcgca
    16201 tcagcagcac cggccagggg cccgtgccgg tggtgggccg ccagatgtcg gcatcgaggc
    16261 ggatgccatc gcgggtggtc atgctggcgc tggtcaggcc gggcagcggc cctgcggttt
    16321 ccccggtcat gcggcatcgg cccttttgaa actgcgcatc ttgcccggat tgaggaggcc
    16381 tgcaggatca accgcctgct tgaagcccag ctgatcgccc ggcaggcgct tgtagcggct
    16441 gccatcctcc accgtcacca catgggggtt ggccacaggt gcgccttccg cctcgaacag
    16501 ggcgatcacc tcggccagcc gctggcgccc gccccagcgc agcaccggca gcgtgttcat
    16561 cgcatggccg ccatcaaagc cgatgaattc cacatgccac atcacctcat cgcccagcct
    16621 ggcgcgcagg gccagcagca cctcggccga agggcacagg ctttgcagat aggtgtagcc
    16681 gcgcgcgcgg cgcagcacat gctgggtggc atggttgaag gcgatctcgt agatcggcag
    16741 gctgtcgggg tcggcctctg ccgcggcatt ggcgcgctgc agggccaccg cgccgcgatg
    16801 ggcggcggcc agttcctcca ccgccaggca gccggccggc gccaccatca ccgccaccac
    16861 cgcatggccc gccgggatct gcccggcaaa gggccggaaa taacccgcca gcgccccgca
    16921 ggagagcgac accagccgct tttccacccc ttgcgcccgg cacagcgcct ggccaaaggc
    16981 gatcgcatcg gccagctctg caaaggtgac gagcatgtcc tgccaatcca ccgcggcgcg
    17041 agtgggaatg gtgagcgcgc tgatcagccc ggtggtgcca aaggtgcggt tgacgagatt
    17101 gcactgctgg cccttcagcg tgaggacacg cggggtctcc tccacggtga tcacctcggc
    17161 ggccagcagg ttgcccggct cgcgcaggcc gccccaatgg acagagccca cgccgcctgt
    17221 gccgcccgcc acaaagccgc ccagcgtggc cgtgtgccag gtcgtgggaa acatgcgcag
    17281 ttcctggccc tcggcccgca ggcgggtttc cagatcgaac atggcgatgc ccgcttccac
    17341 ctccaccgcg cctgcctcca gccggcggat gcgggccagg ccggtgagat cgaccaccgc
    17401 gccgccggcc agcggcacgc attggccata attgccggtg ccgccgccgc gcgccgtcag
    17461 cggcacgccg ctggcagcgc tggccgccgc cacgcgcagc agatccgccc ggtcacgcgg
    17521 ggtgacgatc acctcggcct ccagatgctg cagctcttct gccagcaccg ggctgaacca
    17581 gaaataatcc ttgctcttgc ggcggatctc ggccggatcg cggctgatgg gcacatcgcc
    17641 cagccgttcg aggaaagcgg acagggccat ggccgcctca gccctgctgc acgctggccg
    17701 cccgggcggg cacgccgatc gtctcggccg gatagtccgc cacttcggcg cacaggtcga
    17761 ggaagcggct ggcggcatgt tcggccacca gccggccacg ctcggcatca gcggcggcgg
    17821 cattgccgca ggcgcccttg gggttcagat cttccacctg ccagccaaaa cccaccttgc
    17881 cctcgggcgt gaggatgcgg aaacgctcct gcatcgtctg gctcagcgag acgaaattct
    17941 ccgccttgcc catatccacc agatccgggc gcagatgcag catcagcgag gtctcgatct
    18001 cgccgccatg gatgccgtgt tcgcattcct cggggctgaa caggcccttg ggcatgccca
    18061 tcttcgacca ggcggcgttg acgacgaaca tccccagctc cacccgcagc ccacgcgcga
    18121 cgatatccat cacctgcggc tggccgccgt gggaattgag caggatcagc cggcggatgc
    18181 ccgccctgaa aacgctgcgg ccaatctcca tcagcagccc ggtcagcacc tcggccgaga
    18241 cggtgagcgt gccgggaaaa gagatatgct catcgctctt gccgacggac tgcatcggca
    18301 gggcagtgat cggcaggtct gccggcgccg tcttcagggc gatgtcgagc aggccggcgt
    18361 tgatgcagct gtcgacgaac accggcagat gcggcccgtg ctgctcgatc gcccccaccg
    18421 gcaggatggc gacagtgttg gcggccagct gcccgaaggc cgaggagggc atgtcttgcc
    18481 agtggcgggc gggaatcatc gcaggtctgg tccttcaaac aagggggaaa tcaatagccg
    18541 taggaacggc ggaacatctc gcgcatgtgc tcgccagccg agcacacatc gaagcggggc
    18601 ggattgtcga cgctgatgca ggaggcgatc ggcgcgatgg tggcctcatc gtgcgggctg
    18661 tagaaaaagg gcatcgaata gcgatcgcgc gcggccgcat tgttcagcac ccggtgcatg
    18721 ttcgaggcat agacgccatt cgtccagcgg gccatcagat cgcccagatt gatgacgaac
    18781 gtgccttcca ccggcgtggc cttgatccag cggccctgcc ggtcgcgcac ttccaggccg
    18841 cccagctcat cctgcgccag caccgtgatg ccgccccaat cggtgtgggc ccccgcgccg
    18901 atctggttga aggcggcatc tgcggcctgg ggcggatagc ggatgaagcg cagcttggcc
    18961 gggctgccgg caaagaaggg agagaaccac gcacgcggga gatcgaggct gagtgccagc
    19021 agttccagca gccggtcgcc cagcgcctgc accgcctcgc cataggcgcg ggtgtgggcc
    19081 acctctgcgg ccagccctgc cggccactgg ttgctgccca ccaccctggc ggggctgcga
    19141 gccgaagggt tatcgtccgc caggttggtg cagcaatagt aggcttcctt gagatcgggc
    19201 ggggcctcgg tgccgccatc ctggctgtcg agcacttgcc cgcacagcgc ttcatagccg
    19261 cacaccagcg cggaatggcg catatccagc gccgccttct cggccgccgg cacggcaaag
    19321 aagcgccggg ccgcggcaaa ctggcccgcc accagttcgg gcgcgatgcc atgccccgcc
    19381 gcatagaaga agccggtatc gaggcaggcg gcgcggattt gcgcggccac ctcggtttcc
    19441 gggccggaga tatcgatcac cggcaggctg tcggcctcct ggggcggggc atagatttcc
    19501 atcgggcttg tccttcctca gaagttcttc gtcaacgaca cgccataggt gcgcggcatg
    19561 ttgtagagcg tattgaccgt ggaggagttc tggtaggtcg aattggtata ttcgcaattg
    19621 gtgaggttct tgccccagaa catcatcgac cagcccttgt gcggcagcag cacctggaac
    19681 tgggcgttga ccagcgttgc cgccgtgttc acccggtaaa tcgcggtgtt ctcggtattg
    19741 gcataaacgc tgctctgccg gtgggccgag gcgcccagcg ccagatccac cgcatctgtg
    19801 aggtgccact gatagcggcc agacaccgtg ccggcaaagg aaggcgcatt ggcaaagcgg
    19861 ttgcccgcct tgtagagcac gccgccgctg gtgaaggcgc ccacccaggt atccacccag
    19921 ccgccatcca gcttcagctc aaggccgctg atcgggcgga aggtggccga cacttccagc
    19981 cccttcagat gcgccttggg cagattgccg aagacgttga cattgaggtt gcccaccagc
    20041 tgctgctggt tggtctgcat gttgtgatag acatagtaga acagcgcgcc ttccagatcc
    20101 accttgtgat gggcgagcga ggccttgctg cccacttcga aggcatagat atgctcgccc
    20161 ttgtaggggt tgagctgggc cgaactggtg gcattgggcg aggcagcaaa gccgccgctc
    20221 ttgaagccct cggtcacgct gccatagacc agcgtgttgg catcgggctt gtaatcgagg
    20281 ttggccttcc acgagatggc attctgctgg atggactggt cgagataggc ctgggttgcc
    20341 atgtaggtgg accagtcggc gctgtatttg aaggtgccgc cggccttgtg gtcatgctcg
    20401 tagatgaagc gcgcgccggt gcccagggtc agcttgggcg tgatatgcgt gtcgagctgg
    20461 gcaaagccct gcagggtctg ggtgtaggtg ttgttggtgt tgaacaggtt gtagccgttg
    20521 atcggcgtat aggaataggt gggattgttg acattgccgc tggaggcata ggcgccggcc
    20581 atccagtcga tgaaatgctg gccggtggag ctgaccacca catcctgcga gatctgccag
    20641 gtcttctggt ccagcagata gtgctgctcg gtcaccggca ggccatcgcc cttgttgtaa
    20701 tattcgtgcc accacagata gccggtagtg gccttcacgg tgaagccatc gcccttgtag
    20761 ctgctgatca gcgtgccggt gtaggtgttc gaatgaacca gccagctgtc gttctccgac
    20821 agggtgagca ggttggtgga gccgcgcgtg gcgccccagc tggaagcgca attgccgcca
    20881 aaatcaatct ggttggcatt ggatgtggcg caataggtgg acgacgggct gctgctcgaa
    20941 ccacccgttg tcttgcggcc gaataccgtc atcgcgccgg gcacgtcatt ctcatactga
    21001 tagccgaaga tgccctggaa gctcagccgg tcggtgggct gccacttcaa ctgggcgcgc
    21061 acggcggtct ggtgcagagt gccgatcttc tggccgccgg gataggtgtt gttcatccag
    21121 ccatcataca tctggcgcgt tttcgccgag aggcggccgg tgagattgct ggtgatcggc
    21181 ccggaaatcg ccccttccac attcaccgca tcgtaattgc catagctgac cgtgccatag
    21241 ccggagaggt gatcggtggg ctgggcggaa ttgatgatga ccgcgcccgc tgtcgcattg
    21301 cggccataga gcgtggattg cgggcccttc agcacttcca cctcggccag atcgaacacc
    21361 tgctggctga tcgtgccgat attggggctg tagacgccgt ccatatagac cgaggtggac
    21421 gggttgctgg ccacggtgaa gtcgttcatg ccaacgccac ggatggtgat cgccggctgg
    21481 ccgccgccgg ggcggatctc gcgcacttcc acgttggaag tggcctggat cagctcgccc
    21541 acgcgctcca cgcgctcttc ggtcaggcgc tgggcgctga tcgcggtcac cgccattggc
    21601 acttccagcg ccgactggct gcggcgctgg gcggttacga caatgccgga gccgccctcg
    21661 gcgctggcgg tatcggcgct ggcggcatcg gcgctatctg tggcgccatc ggcggcgtgg
    21721 gccggtgttg tggccaggcc gatggccagc gccatgcccg ctgctgcaat tgcgaaattc
    21781 ctgtctacac gacgcatgtg cttcccccat gctgaatccg gtccatggcc gcaaagcccc
    21841 gccaatggcc ggaaaattga cgaattccac cccgagaacc tgtgcatctt ttgttgtgca
    21901 ctgcgatgct atgcgaagag atcgtgccga aaaagaagca atttggcatt gttttgatgc
    21961 cttaaaggca gcacaaaacc atgccggaaa ggcatcggca tgatgcgatt tgcagctttt
    22021 ggggaaaggg gaaaggaatc cgcgcacgcc tgcgcaaggc cagcaggcgc catggcccgc
    22081 tcacgcaaaa ggcgcgggaa gccatgcccc ccgcgccccg gtcttgtcat ttcacgcgtt
    22141 gatcaccagg cggtggcgcc ggcatcgatg ggaatatccg cccccgtgac atagcgcgcc
    22201 tcgtcagacg ccagccacac cgcgccccag ccgatatcct ccggctcacc aaagaatttc
    22261 agcatgttgc cggccagcac atgctgctcc agcgcaggat cgcgggcgag atgggcgcgc
    22321 gtggcggcgg tcagcgtgaa gccgggcgag atggtgttgg cgcggatgcc aaagggcgcg
    22381 ccctccatcg ccagttgccg ggtcatcgcc agcacgccgc ccttgccggc gcaatgggcc
    22441 agcgccggcg atccgggcag ggcatggcgg gcattggcgc tggcgaaatt gacgatgctg
    22501 gcccgcccgc tggccttcag atgcggccaa gccgcgcggg tgggcaggaa aacgatgtcc
    22561 agctcgccca ccagcgtgcg ctgccactgg gcataggtca tctcctcgat ccaggcgaaa
    22621 gtggcgaagg ccgccgcatt caccagcgca tcgatccgcc cgtgctccgc cccgatgcgg
    22681 gcgaaagtgg cggcaacggc cgcctcgtcc accagatcgc aggcgatgtc gacgcccgcg
    22741 cccgcctcgc ggtcgatgcc gatcaccgtc gccccttcgg cgcggaacag ctgcgccacc
    22801 ccggcgccaa tgccatgggc cgcgcccgtg acgaccgcca gccggcccgc cagccgctca
    22861 gccatggagc gccacccggc cagagccatt gtggccattg ccgttgccat tgccgtcagg
    22921 ctcatccgcc gcgcggccga tccgcccggc caccgccagc accaccacca gccccagcac
    22981 cgcgccggcc gacagcacgc caaaggccgc ctgcaagccg cccgcatgct gcgcggcaaa
    23041 ggtgacgacc accgggctga ggaactggcc aaaggcaaag gcgctctgcc acatgccggc
    23101 gccgcggccg cgcacctcga aggacagcag gctcatcgcc cacaccagca gcgtgggcag
    23161 cagcatgccg gcgcccagct ggttgaggaa acagccgacc aggaagccat tgggcgtgcc
    23221 cgcccgcgcc atcagcagga agcccaccgc cagcagggca aattcgatca gcagcaggcg
    23281 ctgcaccggc cagcggccca cgcgcgagta gatcagcgtg cccagcggca cccccacgct
    23341 ggccaccgag gtgaggaagc ccacccgcgc cggatccagc acgcccagca gcgtaaggcc
    23401 aaggccggcc tggatctgca ccgtatagaa gaacaccgcg ccataaaggg tgatcgccag
    23461 gatcaccgcc atgcggcccc agggaaagcc cgcccagctg gcattgtgcg gcggcgcctc
    23521 ggcctcatcg cccagatcat cggccggctc ccaggtgaag cgcgccacca gcgccagcat
    23581 caccagcgcc gaggcataga cccagaacgg cgtgcgccag ccaaagccgc ccagctgccc
    23641 gcccacgttg aagaacacca gcgccgacat cgaggcgaag gccgtctgcc cggccagcca
    23701 tttgtcacgc gcgggaccgt ggaaataatc gccgatcatc gtggtggaaa gcaccatgat
    23761 cagcgcctcg gccaggccca cgcccacgcg gctgacgaga atggcggtga gatcatgcag
    23821 gaacaccggc gccagcccca ccaccgcata ggcgacgaaa gaggccatca gcagccggcg
    23881 gcggccaaaa taatcgcccg ccgcgcccgc cagcgtggaa aacagcgcca cgcacagcgc
    23941 cgggatcgtc aggatcatcg gcacccaata ttcaaagccg ggcacaccgc tgaattcctt
    24001 gaggatgccc ggcagcaccg gcgccagcag cacgatcgcc atggtggaaa gggtgatcgg
    24061 cagcaacagg gcaacccctg tcatccagcc tggctgatgg tggcgtatca tgcttttcct
    24121 ctctcccggc ggggcccggg gcggcccgca ggaaaggggc acgcacacag gccgccacgc
    24181 gcgactgttc ttggccgcaa cttcggcgag gcggcggggc ttgtctaatc gaaactatgg
    24241 aacagaagcg attgatcgcg cgggattgcg cctgcccgcg ggcgcctcag gcgccatcgt
    24301 ccaccagcgt ttccatcacc acgaaggtgg aggtgctggc cacatgcggc aggctggaga
    24361 tcttctcgcc cagaaccttg cgataggaat ggatatcgcg cgtgcgcact ttcagcagat
    24421 agtcgaaatt ggcggcgatc atgtggcatt cctccacttc ctccacctgc agcacggcgc
    24481 ggcggaattc ctccagcgcc ttctcgcgcg tgtccgacaa gcgcacctcg gcaaaggcga
    24541 tatggcccac gcccagcttg atgggatcga tcaccgccct gaagccgcgg atatagcctt
    24601 cttccaccag ccgcttcagc cgcagctggc agggcgtttt ggaaaggccc acgcgcgcgg
    24661 ccagttcggt gatggtcatc cggccatcgc ggcgcaattg ctcgacaatg cggcggtcga
    24721 tctggtcgat atggctatga tctggcaatc tggcaggcat aatggctgaa ttatgcattc
    24781 aacttggcga tgtggcaaat agcaaagccg atcattaggc agatcgcctg caaagagggt
    24841 gctagcatca cgccatcgcc agcccctttt gcggacagcc ccgatgaccg acacccccgc
    24901 cgcccctttc gcccgctttg ccccgccgat cgccgccgcc gcgcctttgc gcacggcaat
    24961 caccgccgcg cctttaagag cggcgattac cgccgcttgt cgccggcccg agcccgaagc
    25021 gatggctccg ctgatcgccg ccgcccgcct gcccgaaggc accgcccgcg ccatcgccgc
    25081 caccgcctgc acgctgatca ccgccctgcg ccagcgcggc gccggcggcg gcgtggcggc
    25141 gctggtgaag gaatatgcgc tctccagcca ggaaggcgtg gcgctgatgt gcctggccga
    25201 agccctgctg cgcatccccg atgccgccac ccgcgatgcc ctcatccgcg acaagatc
//
LOCUS       DASRYE010000187         6099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_26052,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000187 DASRYE010000000
VERSION     DASRYE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26052"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFF98_09795"
     CDS             complement(<1..1141)
                     /locus_tag="VFF98_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU63963.1"
                     /translation="MIDTLPPAAIVAPAQTPAHAAATAAPAPGDAGTGPAGTGPAAVI
                     TTAAAQSQAAAATAPDSTGSATVAPTSAAAPAPSWRQHLRNHGVDLTFSYQSESAAAV
                     SGGARQGSAYTQQLLASAAIDTGKAFGLKGGKFIFTLIERTGVDLTAKRLGNLFEVQE
                     LYGGGQDVRPANITYEQEFAGGKTALKIGMYNSGADFATLPSGCNFQNFAFCPRPTTL
                     FYNSGFSGFPVPRWGVRLRQQIVPGWYVSAAVYEVNPYRGQMGHGWDLAPRFDSAVVP
                     VEIMYKGGQKPGQLPSLLRFGGLVDTTNKPDVYADANGNDYALSGLPAATRTERWSAW
                     MMGEKGVAHIGPGGAGVLSLFSAVTVSDLHTARVPYYVSAGAVAKG"
     gene            complement(1173..2051)
                     /locus_tag="VFF98_09800"
     CDS             complement(1173..2051)
                     /locus_tag="VFF98_09800"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZU63964.1"
                     /translation="MQHVVLSPDARPHTFLCGLLGAGIGASRSPELHEREGAALGLAL
                     VYRLLDAEVMGYGAADLPGLLAMLGQMGFAGVNVTHPFKQAAAELMDELSDAARALGA
                     VNTVVWREGRLFGDNTDWSGYRAHFAAALGDRPHGRVALIGAGGAAAAVGYAHLDLGA
                     EELRIFDTSAERAAALAARLGALFPAAAVRAVASAAEAITGADGVAQTSPVGMQGHAG
                     LPFDPALLAPAMWVSDIIYFPAETALLAAARARGCATLNGAGMVVRQAADALARFTGH
                     QPDTARMLAAFHQHTP"
     gene            complement(2079..3434)
                     /locus_tag="VFF98_09805"
     CDS             complement(2079..3434)
                     /locus_tag="VFF98_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63965.1"
                     /translation="MASFSASGTVSVRPFVTVEGQQAPARDGRWSRLQVLVVALCFVL
                     NMLDGMDVLILSYIAPALSADWHLAPDQMGTVFSAGLAGMAAGGLLLAPMADRFGRRR
                     LILVSLLVMTAAMFGSVFCHSLWQLLAARFAVGVGIGSVLASMAALTAEYAPAHHRAF
                     AVGFLQAGYPVGATITGFVVAAHLATHGWQAMLLGAAVLCALALPVVFLLLPESIAFL
                     AARQPKGALAEINRQLAQMGEAPLDALPPRRDGAEAPHTGLRGLMAEGRLPGTLLLWA
                     AIIFSFMTLYFVISWIPKLSIEAGLSPRHGIYAGAIYNIGAFIGTSSVGLVAMRLELR
                     KVILGYMVLAALALVAFGSLHMPLALTLLTAFLIGLFVQGGFNGCYPLATGFYPAEVR
                     SAGLGWAMGMGRIGAVIGPMLGGYLLTAKVALPVIFGVFAAPIVVAGLLVVLIRQPAR
                     S"
     gene            complement(3582..4496)
                     /locus_tag="VFF98_09810"
     CDS             complement(3582..4496)
                     /locus_tag="VFF98_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU63966.1"
                     /translation="MLTMRQIEMFRAVMIAKTISGAARLLDISQPGISRMLAHMEDKV
                     RFRLFDRTRGRLIPTHEAQVLFEQIQRFYEGFGELDDTIRRLAKGEDRTFRIGASPSL
                     GHSVVPAMLARLTANYPGLMLQFDILSVDQARTYLASQRGEYALTLFEIDHPNVLSSR
                     IAAARLVCAVRADHPLAARESIGVADIADEPILSFPPDTPHGRAIARMYEEAGCELDV
                     ATYVRFAETAVAFVAQGMGLALVDSFTAMQPHDPGVRFLEFEAPGTLPVYLNRNLDMP
                     RAMIGETFEEIARKVLSALPRPKPPVRG"
     gene            complement(4530..5189)
                     /locus_tag="VFF98_09815"
     CDS             complement(4530..5189)
                     /locus_tag="VFF98_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU63967.1"
                     /translation="MGKPLSRREERRGERRAAILETATQIFKDHGYAGTTMSGIAALL
                     GGSKGTLWSYFCGKDELFAACIEEMTRAFREDLLKVLDPAAPLRPAVEGFCQRFIAKI
                     LAPDAIAFYRLLCGECGRAPEISRIFHERGPAVIEAMLIGFLTSHLAAGRLRGGTAME
                     MANFLFSMCMGMAHSRALLGIEPLPGPTPEAIAATFFRLYGGNGPEGALTEAGVGEGA
                     V"
     gene            5288..>6099
                     /locus_tag="VFF98_09820"
     CDS             5288..>6099
                     /locus_tag="VFF98_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU63968.1"
                     /translation="MHTPRLPHCLITAALLPALLAGCVSAPKLGPKPAPRPLAAYAAT
                     QTLAATPGASAPAADWWRAYADPQLTALIEEGLASAPDVAIAVARLRQAEGYHQQAGA
                     ALLPRLDAKVLGQEAYIHDFPGGIAPNGWAGAGAEALQLSFDIDLWGKNRAALRAARH
                     EDEAAALDLAQARLTLSTAIASSYADLARLYAERDVQAQAVQVKENTRRMVADRVANG
                     LDTQAELKQAEAAVPTAQADLLATDEQIALARNTLAALLGKGPDRGLAIARPA"
BASE COUNT         1069 a         2130 c         2044 g          856 t
ORIGIN      
        1 aacccttggc caccgcgccc gccgacacgt aatagggcac gcgcgcggta tgcaggtccg
       61 acacggtgac ggcgctgaac aggctgagca cgcccgcgcc ccccgggccg atatgcgcca
      121 cgcccttctc gcccatcatc caggcgctcc agcgttcggt gcgggtggca gccggcaggc
      181 cagacagggc ataatcattg ccattggcat cggcatagac atcgggcttg ttggtggtgt
      241 ccaccagccc gccaaagcgc agcaggctgg gcagctggcc cggcttctgc ccgcccttgt
      301 acatgatctc caccggcacc accgccgaat cgaagcgcgg cgccagatcc cagccatggc
      361 ccatctgccc gcgatagggg ttcacttcat aaaccgcggc cgacacatac cagcccggca
      421 cgatctgctg gcgcagccgc acgccccagc gcggcaccgg aaagcccgag aagccgctgt
      481 tgtagaacag cgtggtcggc cgcgggcaga aggcgaagtt ctggaaattg cagcccgagg
      541 gcagcgtggc gaaatcggcg ccgctgttat acatgccgat cttcagcgcg gtcttgccgc
      601 cggcaaattc ctgctcgtag gtgatgttgg ccgggcgcac atcctgcccg ccgccataca
      661 gttcctgcac ctcgaagaga ttgcccagcc gtttggcggt cagatccacg ccggtgcgct
      721 cgatgagggt gaagatgaac ttgccgcctt tgaggccaaa ggccttgccg gtgtcgatgg
      781 cggccgaggc gagcaattgc tgcgtatagg ccgaaccctg gcgggcgccg cccgaaacgg
      841 ccgccgcgct ttccgactga taggagaagg tgagatcgac gccatggttg cgcaggtgct
      901 ggcgccacga gggcgcgggc gcagccgcgc tggtgggcgc caccgtggcc gagccggtgc
      961 tgtcgggcgc ggtggccgca gcggcctgcg attgcgcggc agcggtggtg ataaccgcgg
     1021 cagggccagt gccggctggg ccagtgccgg catcaccggg cgcgggcgcg gccgtggcgg
     1081 cggcatgtgc aggggtttgc gccggggcga cgatggccgc cggcggaagg gtgtcgatca
     1141 tgttttatcc tctccctggt gtgcagggtg tatcaggggg tgtgttggtg gaaagcggcg
     1201 agcatccgcg ccgtatcggg ctggtggcca gtgaagcggg ccagcgcatc ggcagcctgg
     1261 cgcaccacca tgccggcgcc gttcagcgtg gcgcagccgc gcgcgcgcgc ggcggccagc
     1321 aaggcggttt cggcggggaa atagatgatg tcggacaccc acatggcagg ggccagcagg
     1381 gccgggtcga acggcaggcc ggcatggccc tgcatgccca ccggcgaggt ttgcgccacg
     1441 ccatcggcgc cagtaatcgc ctcggcggcg ctggccacgg cgcgcacggc ggcggccgga
     1501 aacagcgcgc ccaggcgggc ggccagcgcg gccgcgcgct cggcgctggt gtcgaaaatg
     1561 cgcagctctt cagcgccaag gtcgagatgg gcatagccca ccgccgccgc cgccccgcca
     1621 gcgccgatca gcgccacccg gccatgcggc ctgtcgccca gcgcggcggc aaaatgggcg
     1681 cgatagcccg accagtcggt attgtcgcca aacaggcgcc cctcgcgcca caccacggtg
     1741 ttcaccgcgc ccagcgcccg cgccgcatcc gacagctcgt ccatcagctc ggcggcggcc
     1801 tgcttgaagg gatgggtcac attcaccccg gcaaagccca tctgccccag catggccaac
     1861 aggcccggca gatccgccgc gccatagccc atcacctcgg catcgagcag gcgatagacc
     1921 agggccaggc ccagcgcggc gccctcgcgc tcatgcagct cggggctgcg cgaggcgccg
     1981 atgccagcgc ccagcaagcc gcacagaaag gtgtggggcc gggcatcggg cgagaggacg
     2041 acatgctgca tgggggaagg ctttcgtgtg ggaggagatc aggaacgggc cggctggcgg
     2101 atcagcacta ccaacaggcc ggccaccacg atgggcgcgg caaacacacc gaagatcacc
     2161 ggcagcgcca ccttggcggt cagcagatag ccgcccagca tcgggccgat caccgcgccg
     2221 atgcggccca tgcccatcgc ccagccaagg ccggcgctgc gcacttcggc cggatagaag
     2281 ccggtggcca gcggatagca gccgttgaag ccgccctgca cgaacaggcc gatgaggaaa
     2341 gcggtgagca gcgtgagcgc cagcggcata tgcagcgatc cgaaagccac cagcgccagc
     2401 gccgccagca ccatatagcc caggatcacc ttgcgcagtt ccagccgcat cgccaccagc
     2461 cccaccgagg acgtgccgat gaaagcgccg atattgtaga tggcgccggc atagatgccg
     2521 tgccgggggc tgaggcccgc ctcgatcgac agcttgggga tccagctgat gacgaaatag
     2581 agcgtcatga agctgaagat gatcgccgcc cacagcagca gcgtgccggg caggcgccct
     2641 tcggccatca gcccgcgcag gcccgtgtgc ggggcctcgg cgccatccct gcgcggcggc
     2701 agggcatcaa gcggggcctc gcccatctgc gccagctggc ggttgatctc tgccagcgcg
     2761 cccttgggct ggcgggcggc aaggaaggcg attgattcgg gcagcagcag gaacaccacc
     2821 ggcagcgcca gcgcgcacag caccgcggcg cccagcagca tcgcctgcca gccatgggtg
     2881 gccagatgcg ctgccaccac aaagccggtg atcgtggccc ccaccggata gcccgcctgc
     2941 aaaaagccca cggcaaaggc gcggtgatgg gccggcgcat attcggccgt cagcgcggcc
     3001 atgctggcca gcacgctgcc aatgcccacg cccacggcaa agcgcgcggc cagcaattgc
     3061 cacaggctgt ggcagaacac cgatccgaac atcgccgccg tcatcaccag cagcgagacg
     3121 aggatcagcc ggcgccggcc aaaccggtcg gccatggggg ccagcagcag gccgccggcg
     3181 gccatgccgg ccagccccgc gctgaacacc gtgcccatct ggtcgggcgc caggtgccag
     3241 tctgccgaca gcgccggggc gatataggag aggatcagca catccatgcc atccagcatg
     3301 ttgagcacga agcacagcgc caccaccagc acttgcagcc ggctccagcg gccatcgcgc
     3361 gcgggcgcct gctggccttc aacggtgaca aacgggcgca cggaaaccgt gccgctcgca
     3421 gagaagctcg ccatgtgaga gacgcctttc atcgctctcc accgccgggt ggcctggcca
     3481 tgcccgcagt ggtcatttat attctggtta aggattatgc gccttgccac aggcgggcgc
     3541 cagcaccgcc agcgccttcg cccatgccct tatgttatag cttagccgcg caccggcggc
     3601 ttgggcctgg gcagggcaga aagcaccttg cgggcgattt cctcgaaggt ttcaccgatc
     3661 atcgcgcggg gcatgtcgag gttgcggttg agatagaccg gcagcgtgcc cggcgcctcg
     3721 aattcaagga agcgcacgcc cggatcatgc ggctgcatgg cggtgaaact gtccaccagc
     3781 gccagcccca tgccctgcgc cacaaaggcc accgccgttt cggcaaagcg cacataggtg
     3841 gccacatcca gctcgcagcc ggcctcctca tacatgcggg cgatggcgcg gccatggggc
     3901 gtgtcgggcg ggaaggagag gatcggctcg tcggcgatat cggccacgcc aatcgattcg
     3961 cgcgcggcca gcgggtgatc cgcccgcacc gcgcacacca gccgtgccgc cgcgatccgg
     4021 ctggagagca cgttgggatg gtcgatctcg aacagggtga gcgcatattc gccgcgctgc
     4081 gaggccagat aggtgcgcgc ctgatcgacc gagagaatat cgaactgcag catcagcccg
     4141 gggtaattgg ccgtgagcct ggccagcatc gccggcacca ccgaatggcc cagcgatggc
     4201 gaggcgccaa tgcggaaggt gcggtcctcg cccttggcca ggcggcggat ggtgtcgtcc
     4261 agctcgccaa agccctcgta gaagcgctgg atctgctcga acagcacctg cgcctcatgg
     4321 gtggggatca gccggccgcg ggtgcggtcg aacaggcgga agcgcacctt gtcttccata
     4381 tgcgccagca tgcggctgat gccgggctgg gaaatatcca gcaggcgggc cgcgccgctg
     4441 atcgtcttgg cgatcatcac ggcgcggaac atctcgatct ggcgcatggt cagcatggcc
     4501 ggcgtgctac cacagcccgg ccggccggat caaacggccc cttcccctac tcccgcctcc
     4561 gttagcgcgc cttccgggcc attgccgcca taaagccgga agaaagtggc cgcgatcgcc
     4621 tcgggcgtgg ggcctggcag cggctctatg cccagcaatg cgcgcgaatg ggccatgccc
     4681 atgcacatgg aaaacaggaa attggccatt tccatcgccg tgccgccgcg cagccgcccg
     4741 gccgccagat ggctggtgag aaagccgatc agcatcgcct cgatcaccgc ggggccgcgt
     4801 tcgtggaaga tccggctgat ttccggcgcc cgcccgcatt cgccgcaaag cagccggtag
     4861 aaggcaatcg catcgggcgc cagaatcttg gcgatgaacc gctggcagaa gccttccacc
     4921 gccgggcgca agggcgccgc cggatccagc accttcagca gatcctcgcg aaaggcccgc
     4981 gtcatttcct cgatgcaggc ggcaaacagc tcgtccttgc cgcagaaata gctccacaac
     5041 gtgcccttgg aaccgcccag aagggcggca atccccgaca tggtggtgcc ggcgtagcca
     5101 tggtccttaa aaatctgtgt ggcagtctca agaatggccg cgcgcctctc accgcgccgc
     5161 tcttcccgcc tgctcagggg tttgcccata cgttcatcct tttcccgtac tatatggtac
     5221 gattttctcg ttgacaagaa tcaatcgatg ggagatacga gccgtaccac atagtacggt
     5281 atttatcatg cacacccccc gcctccccca ttgcctgatc accgcggcgc tgctgcccgc
     5341 cctgcttgcc ggctgcgtgt cggcgcccaa gctgggcccc aagccggcgc cgcgcccgct
     5401 ggccgcctat gccgccacgc aaacgctggc cgccaccccc ggcgccagcg cgcctgcggc
     5461 agactggtgg cgcgcctatg ccgacccgca gctgaccgcg ctgatcgagg aagggctggc
     5521 cagcgcgccc gatgtggcga tcgccgttgc ccgcctgcgc caggccgaag gctatcacca
     5581 gcaggccggc gccgcgctgc tgccgcgcct ggatgccaag gtgctgggcc aggaagccta
     5641 tatccacgac ttccccggcg ggatcgcccc caatggctgg gcgggcgccg gcgccgaggc
     5701 gctgcagctg agcttcgaca tcgacctgtg gggcaagaac cgcgccgccc tgcgcgccgc
     5761 ccgccacgag gatgaggccg ccgcgctgga tctggcgcag gcccgcctca cgctttccac
     5821 cgcgatcgcc agttcctatg ccgatctcgc ccgcctttat gccgagcgcg atgtgcaggc
     5881 gcaggcggtg caggtgaagg aaaacacccg ccggatggtg gccgaccgcg tggccaacgg
     5941 gctggacacc caggccgagc tgaagcaggc cgaggccgcc gtgcccaccg cccaggccga
     6001 tcttctggcc accgacgagc agattgccct ggcccgcaac acgctggccg cccttttggg
     6061 caaggggcca gaccgcgggc tggcgattgc ccgcccggc
//
LOCUS       DASRYE010000188         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_152349,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000188 DASRYE010000000
VERSION     DASRYE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152349"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3197
                     /gene="putA"
                     /locus_tag="VFF98_09825"
     CDS             <1..3197
                     /gene="putA"
                     /locus_tag="VFF98_09825"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HZU63969.1"
                     /translation="AGGDWQAHLGAGPSVFVNAATWGLVVTGHLLASETPSSASLGAT
                     LTRLIGRAGEPVIRRAVDVAMRLMGAQFVTGETIAEALKRARKLEAQGFAYSYDMLGE
                     AATTAADAARYLADYEGAIHAIGQASAGRGIHAGPGISIKLSALHPRYARAQAARVMA
                     ELLPRVRALALLAKRYDIGLNIDAEEADRLEISLDLLEALALDAELAGWNGLGFVVQA
                     YGKRCPFVIDWLVDLAHRAGRRIMVRLVKGAYWDSEIKRAQSEGLADFPVYTRKIHTD
                     VAYIACARKLLAARGALFPQFATHNAQTLASIYHLAGPDFTPGDYEFQCLHGMGEPLY
                     EQVVGAARLNRPCRIYAPVGTHETLLAYLVRRLLENGANSSFVHRIWDASLSPEALAE
                     DPVAAALQVAPVGAPHPLVALPGALYGPRANAAGLDLADETTLAALSAALQASAAQGA
                     QALPEGTSGPMRPITNPADRRCVVGHVREACAADIAPALARAEAAFPAWAALGAGPRA
                     ALLERAATAMESAAPRLIDLAIREAGKTAANAVAELREAVDFLRYYAAEARRTLSAAT
                     PPLGPVACISPWNFPLAIFTGQVAAALVAGNTVLAKPAEETPLMAAEAVRLLHAAGIP
                     ADVLQLLPGDGAVGAGLVAHEGTAAVLFTGSTEVARLIQRQLAARGLPQGRAIPLIAE
                     TGGQNAMIVDSSALAEQVTADVLASAFDSAGQRCSALRVLCLQEDIAERQLAMIRGAL
                     AEQALGQPDALATDIGPVISEEARAGIEAHVARMAAAGCAITRLPLPADCAHGSFVAP
                     TLIEITDIAEIGGEVFGPVLHVLRYPRAGLAALVEAINATGYGLTFGLHTRLDETVAQ
                     VTQSVAAGNLYVNRNMIGAVVGVQPFGGRGLSGTGPKAGGPLYLGRLVAARPAAAIGR
                     EGPLAGHRAELPGPVGERNLYALHPRGAVLALAHTAAGLEAQIAAALAEGNVLQVLAR
                     PGLALVVAAQPASRVQLIERLPETSAFAAALVEAGPNAHADLIALAAMAGAIPLTQVS
                     AADGTYRADWLVEEQTVSINTTAAGGNASLMALV"
     gene            complement(3224..>4000)
                     /locus_tag="VFF98_09830"
     CDS             complement(3224..>4000)
                     /locus_tag="VFF98_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZU63970.1"
                     /translation="TRHLTLAADGTPLAEGETRADALSDFLVELHQTLKADDKGGWAI
                     GIGGAGLAVMLALGLVAVWPRRGRFVRALRPGPVASARVRPLAWHRALGLWAAAPALL
                     MATTGTAMVFDDGFRALLGGARPDHASHPGPVRVDLAAALATALAAAPGSRLTAIIGM
                     PTAADATYRFRLLAPGEWRRAYGTSMVDIDAGTGAVALLYTPATATFGQRLADSLYAL
                     HTGEVAGWPGRLLVTATGLWLVVMIALGFASWWRRRGQAS"
BASE COUNT          574 a         1442 c         1420 g          564 t
ORIGIN      
        1 tcgccggcgg cgactggcag gcgcatctgg gcgccgggcc ttcggtgttc gtcaacgcgg
       61 ccacctgggg gctggtcgtc accggccatc tgctggccag cgagacaccc tcctcggcca
      121 gccttggcgc caccctcacc cggctgatcg gccgcgccgg cgagccggtg atccgccgcg
      181 cggtggatgt ggcgatgcgg ctgatgggcg cgcagttcgt caccggcgaa accattgccg
      241 aagcgctgaa acgcgcccgc aagctggaag cgcagggctt tgcctacagc tacgacatgc
      301 tgggggaagc tgccaccacc gctgccgatg ccgcccgcta tctggccgat tacgaaggcg
      361 ccatccatgc catcggccag gcctctgccg gccggggcat ccatgcgggg ccgggcatct
      421 cgatcaagct ttccgcgctt catccgcgct atgcccgggc gcaggcggca agggtaatgg
      481 ccgaactgct gccgcgcgtg cgggcgctgg cgctgctggc caagcgctat gatatcggcc
      541 tcaacatcga tgccgaggaa gccgaccggc tggagatcag cctcgacctc ctcgaagcgc
      601 tggcgcttga tgccgaactg gcgggctgga acgggctggg ttttgtcgtg caggcctatg
      661 gcaagcgctg ccccttcgtg atcgactggc tggtggacct ggcccacagg gccggccggc
      721 ggatcatggt gcggctggtg aagggcgcct attgggacag cgagatcaag cgcgcgcaaa
      781 gcgaggggct ggccgatttt cccgtctaca ccaggaagat ccacaccgat gtcgcctata
      841 tcgcctgcgc ccgcaagctg ctggccgcgc gcggcgccct cttcccccag ttcgccaccc
      901 ataatgcgca aacgctggcc agcatctatc atctggccgg gcccgatttc acgccgggcg
      961 attatgagtt ccagtgcctg cacggcatgg gcgagccgct gtatgaacag gtggtgggcg
     1021 cagcacggct gaaccgcccc tgccgcatct atgcgccagt gggcacgcat gaaacgctgc
     1081 tggcctatct ggtgcggcgg ctgctggaaa atggcgccaa ttcctccttc gtccaccgca
     1141 tctgggatgc cagcctaagc cccgaggcac tggccgaaga cccggtggca gcggccctgc
     1201 aggtggcgcc tgtgggcgcg ccccatccgc tggtggcgct ccccggcgcg ctttatggcc
     1261 ccagggccaa tgcggcgggg ctggatctgg ccgacgagac cacgctggcg gccctgtctg
     1321 ccgcgctgca ggccagcgcc gcgcaagggg cgcaagccct gccggaaggc accagcggcc
     1381 ccatgcgccc catcaccaac ccggccgacc ggcgctgcgt ggtgggccat gtgcgcgagg
     1441 cctgcgcggc cgacatcgcc ccggccctcg cccgcgccga agctgccttt cccgcctggg
     1501 cagcgctggg cgcagggccc cgcgccgccc tgctggaacg ggccgccacg gccatggaaa
     1561 gcgccgcacc ccggctgatc gaccttgcca tccgcgaggc cggcaagacc gccgccaatg
     1621 ccgtggccga actgcgcgag gcggtggatt tcctgcgcta ttacgcggcc gaggcgcgcc
     1681 gcaccctgtc tgccgccacg ccgccgctgg ggcctgttgc ctgcatcagc ccgtggaatt
     1741 tcccgctggc gatcttcacc ggccaggtgg ccgccgcgct ggtggcgggc aacaccgtgc
     1801 tggccaaacc ggccgaggaa accccgctga tggccgcaga agcggtgcgc ctgctgcatg
     1861 cagcgggcat cccggccgat gtgctgcaac tgctgcccgg cgatggcgcg gtgggcgcag
     1921 gcctggtggc gcatgaaggc acagcggcag tgctgttcac cggctccacc gaagtggccc
     1981 ggctgatcca gcggcagttg gcggcgcgcg gcctgccgca gggacgcgcc attcccctta
     2041 tcgccgagac cggcgggcag aatgcgatga tcgtcgattc ctcggcgctg gccgagcagg
     2101 tgaccgccga tgtcctcgcc tcggccttcg acagcgccgg ccagcgctgt tcggcgctgc
     2161 gggtgctgtg cctgcaggag gatatcgccg agcgccagct ggcgatgatc cgcggcgcgc
     2221 tggccgagca ggccctgggc cagcccgatg cgctggccac cgacatcggc ccggtcatat
     2281 ccgaggaagc gcgcgccggg atagaggcgc atgtggcgcg catggccgcg gccggctgcg
     2341 ccatcacccg gctgccgctg ccggcagact gtgcccatgg cagctttgtc gcccccaccc
     2401 tcatcgagat caccgatatc gccgagatcg gcggcgaggt gttcggcccg gtgctgcatg
     2461 tgctgcgcta cccccgcgcg gggctggcag cgctggtgga agcgatcaac gccaccggct
     2521 atggcctcac cttcggcctc cacacgcggc tggacgagac cgtggcgcaa gtgacgcaaa
     2581 gcgtggccgc cggcaatctc tacgtcaacc gcaacatgat cggcgcggtg gtgggggtgc
     2641 agccctttgg cgggcggggg ctttccggca ccggccccaa ggcgggcggg ccgctctatc
     2701 tgggccggct ggtggccgcg cggccggccg cagcgatagg ccgcgagggc ccgctggccg
     2761 gccaccgcgc cgaactgccc ggccctgtgg gcgagcgcaa cctctatgcg ctgcatccgc
     2821 gcggggcggt gctggcgctg gcgcatacgg cagccggcct tgaagcgcag atcgccgcgg
     2881 ctctggcaga aggcaacgtg ctgcaggtgc tggcccgccc cggccttgct ctggtagtgg
     2941 ccgcccagcc cgccagccgg gtgcagctga tcgagcgcct gcccgaaacc agcgcctttg
     3001 ccgccgcgct ggtggaagca gggccaaacg cccatgccga cctcatcgcc cttgccgcca
     3061 tggcaggggc cattccgttg acgcaggtga gcgcggccga tggcacctat cgcgccgact
     3121 ggctggtgga ggagcagacc gtgtcgatca acaccaccgc ggccggcggc aatgccagcc
     3181 tgatggcgct ggtttaactg gcgctggttt aactggcgct ggtttaacta gcctggccgc
     3241 gccggcgcca ccacgaggca aagcccagcg cgatcatcac caccagccac aggccggtgg
     3301 ccgttaccag caggcggccc ggccagccgg ccacctcgcc ggtgtggagg gcatagaggc
     3361 tgtcggccag gcgctggccg aaggtggcgg tggcgggcgt gtagagcagc gccaccgcgc
     3421 ccgtgccggc atcgatatcg accatgctgg tgccataggc gcggcgccat tcgcccggcg
     3481 ccagcaggcg gaagcgatag gtggcatcgg cagccgtggg catgccgatg atcgccgtca
     3541 accggctgcc gggggcggcg gccagcgccg ttgcgagcgc cgctgccaga tccacccgca
     3601 ccgggccggg gtggctggca tgatcgggcc gggcgccgcc cagcagcgcg cgaaagccat
     3661 cgtcgaacac catggcggtg ccggtggtgg ccatcagcag ggcgggcgcg gcggcccaca
     3721 ggcccagcgc ccggtgccag gccagcggcc gcacccgcgc gctggccaca gggccggggc
     3781 gcagggcccg cacaaaccgc ccgcgccggg gccacaccgc caccagcccc agcgccagca
     3841 tcaccgccag ccccgccccg ccgatgccga tggcccagcc gcccttgtcg tcggccttca
     3901 gcgtctggtg cagctccacc agaaaatcgg atagcgcatc ggcccgcgtc tcgccctcgg
     3961 ccagcggggt gccatcggcg gccagggtga ggtggcgggt
//
LOCUS       DASRYE010000189        16179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_256201,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000189 DASRYE010000000
VERSION     DASRYE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256201"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFF98_09835"
     CDS             <1..990
                     /locus_tag="VFF98_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZU63971.1"
                     /translation="IWLQGFAEAHDTIGGVYVGVRYGRQEFTDGPNLMVSQRDNNNIR
                     QVENGLRLWARGRTLRADLFDLHMTAYGIDGTGDDLFDNTRRFSGMTLGWVVPRSLLG
                     GSKLYLDPFLWRYRNTLSTWGPNKGNEMRTYAGAHFWGEAGRATIDWTVNHQGGDYEG
                     RAIDAWQLFLAQSFRLGEKPSAPRIGFHADYATGGGAYGSGKLKDAFSPFGNNIYYSY
                     GLFLTPTNMRVLSPNITFQPLRKLRITGEYEWVWRNNAQDAVYRSNGTPYAGTQKVGG
                     HEVGRVMRAQATLSITPRLSFTGRAEEMYAGDVLRRAGYANSTFLAGWLSYRF"
     gene            complement(1001..1687)
                     /locus_tag="VFF98_09840"
     CDS             complement(1001..1687)
                     /locus_tag="VFF98_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZU63972.1"
                     /translation="MPVEPRSALLPGLLLVLSVGTGLVDAISVLGLGKVFTANMTGNI
                     VFLGFAAVATPGFHPLSYVVAIGAFLAGALGAGRIGKRHGAGPLRGWLLRAALVEAGL
                     LWAAAGLATGFTPATQSPAASLLGIVALTAMAMGFRNATIRQLKVPDLTTTVLTLTLT
                     GLAADSALAGGGNPNLGRRVASVLAIFLGAALGAFMVAHGGLAPPLVLAGALVLAETA
                     LCALHPASAA"
     gene            complement(1729..3168)
                     /locus_tag="VFF98_09845"
     CDS             complement(1729..3168)
                     /locus_tag="VFF98_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZU63973.1"
                     /translation="MRASPALAAPLAAALALLAPAAARADPPAESWQPPVLTPERYNE
                     DWSSLADPAGRTGHWTEAFKYIPLDGTGSAYLTTGGELRLRDETYHANLWGQASAPDN
                     GYLWARLLPYADLHVGQLRAFVQPVLGYAVGVRPAPSAIDQTRADLLQAFADVDVPLG
                     GQGGGQGGASLRLRAGREMVGLGTERLVGTRYGPNLPLAFDGGRAIFHTGAFTLNAFY
                     LLPVEAGPNSFDDHSSPTRRLWGAYATQAFGAGGVDLYYLGYRNSAASFQQGRGRELR
                     HTLGLRWFGTTTGRAAGWHWNMEGMAQFGRFADSTIGAWSIASEVGHRFAGARLHPDF
                     TLRANIASGDRSASDHRLGTFNALFPKGKYFGELSPVGPYNLVNLQPVLALDCGRGVG
                     ASLSGGAYWRQSRGDGVYDVPGHLLRAGNAAAARFVGTQEEATLAWRATAELTLSASF
                     SLFQAGRFLAETGAAKTITMTGIEANFRM"
     gene            complement(3172..3591)
                     /locus_tag="VFF98_09850"
     CDS             complement(3172..3591)
                     /locus_tag="VFF98_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZU63974.1"
                     /translation="MSQPAPPRPVAALLGWRGTGFLARLALCGAYLLGGLTKLADFPA
                     AVAEQAHMGLAPPALFAALTIAVELIGPVLVLSGRLVWLGAGMLGVFTALAALVANRF
                     WAMPPGAERFAATNAFFEHGGLVGGFVLVALLAGRGD"
     gene            complement(3588..4484)
                     /locus_tag="VFF98_09855"
     CDS             complement(3588..4484)
                     /locus_tag="VFF98_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HZU63975.1"
                     /translation="MKRLALFLALALASPAMAAPAATPDYAVGPQYDTTHVYVPASDF
                     DRFVASFLATFGGTASKQGEFQVTPTPSLTKSQLVLSPVGTVSVFGFKTEIPYPFGAE
                     RTGYLVSDMDGAVAAAVQHGASRLITTFPDPIGRDVVIQWPGGVNMQLYWHTAKPSYA
                     PLASVPENRIYLTADAADGFVKDWLAFAHGKVVADERGLAGDAIGQPGKTIRQIAIES
                     GYGRMLVIVSDGQLPWPYGRDLTGYAVADLPATLARAAAAGVEVLVPAHIGAGRHSAI
                     VRFPGGYIAEIHAAEIHGAAGQ"
     gene            complement(4514..6358)
                     /locus_tag="VFF98_09860"
     CDS             complement(4514..6358)
                     /locus_tag="VFF98_09860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU63976.1"
                     /translation="MTDLLLINARVTTLDRANPQAEAVAIRDGRILVAGSEADARAAV
                     PQAQIIDAGGRRLIPGLIDSHIHVIRGGLNYNMELRWEGVPSLAEAMAMLKRQAEVTP
                     PPQWVRVVGGFTEHQFAEKRLPTIDEINAAAPETPVFLLHLYDRALLNAAALRAVGYT
                     KDTPNPPGGEIMRDAAGNPTGLLLAQPNATILYATLAKGPKLPQEYQLNSTRQFMREL
                     NSLGVTSVIDAGGGSQNYPDDYEVIEKLHADGLLTLRIAYNLFTQKPKEELEDFANWS
                     KQVKPGDGDDTYRHNGAGEMLVYSAADFEDFRQARPDMPPTMEGDLEPVIRLLAERRW
                     PWRLHATYDETISRALDVFEKVNRDIPLHGLNWFFDHAETISHRNIDRIAALGGGIAV
                     QHRMAFQGEYFVERYGAKAAEATPPIKRMMEAGIPVGAGTDATRVASYNPWVSLSWLV
                     TSRTVGGLALSPVRNRLDRETALRLWTEANTWFSNEVGRKGQIKAGQLADLALLSDDY
                     FAVPESAISQIRSVLTILGGKVVHGNGDYAAHAPDLPRPMPDWSPVGTFGGHWQSPET
                     RWKLASACACAVPCAVHGHGHAAALGAQTPTADPQSFWGALGCGCWAV"
     gene            complement(6374..6649)
                     /locus_tag="VFF98_09865"
     CDS             complement(6374..6649)
                     /locus_tag="VFF98_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HZU63977.1"
                     /translation="MKPYLISLAAGVLVGAIYALLGVRSPAPPTIALIGLLGMLLGEQ
                     AVPVAARLLRGQPVLAFVKTDCARRVLGEHAAPHQAAPHQTTPQRDA"
     gene            complement(6766..7425)
                     /locus_tag="VFF98_09870"
     CDS             complement(6766..7425)
                     /locus_tag="VFF98_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZU63978.1"
                     /translation="MTLTATPTPAKALIAPDNHALVLIDFQSQMAFATKSIDTTLLRN
                     NAGLIAGAAAGFGVPTILTTVAEKSFSGPMFSEVTDPFPGQPLLDRTSMNTWEDAAVI
                     EEVNRIGLGRLVFAGLWTSVCIVGPTLSAIEQGYEVYVITDACGDISAEAHERAVERM
                     IQAGAHPMTALQYLLELQRDWARGETYAFTTGLARELGGAYGLGITYAKTMFGASEGG
                     H"
     gene            complement(7517..8374)
                     /locus_tag="VFF98_09875"
     CDS             complement(7517..8374)
                     /locus_tag="VFF98_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU63979.1"
                     /translation="MTDTLSRPGSARLVTADGVELFYKDWGDSAAPPIVFHHGWPLTG
                     DEWDAQMLFFLQQGYRVIAPDRRGHGRSGQTDTGNDMDTYAADVAALARHLDLRGAIH
                     VGHSTGGGEVAHYAARAEPGRVARIVLIGAVPPIMLQSPSHPAGLPLAVFDGFRAALA
                     ANRAQFFLDVASGPFYGFNRPDAAVSPGLIENWWRQGMMGGAKAHYDCIKAFSETDFT
                     ADLQVIDVPVLILHGEDDQVVPLAASARLSVQLVQQGTLKTYPGLSHGMASTHPDLIN
                     ADLLAFFRS"
     gene            complement(8578..9468)
                     /locus_tag="VFF98_09880"
     CDS             complement(8578..9468)
                     /locus_tag="VFF98_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZU63980.1"
                     /translation="MDHVSTTRHTMNVWGGADCSLVRFDLAGGAQLDIDEMIHDDIVF
                     LAFEGCGWWSRNGDGARQERPGDIIMRRAGEHFAIRSEWIAQTGGVCREIHLSHRRLA
                     EWAELEASPVARLDFGDGLLADGALSRQLLALHARFEQNHEELEAFEGIAAWMEQLAA
                     RLSPAAGQARMGACQRGIARVVDYLRTHYAENVALEDLAGVAGMNRFVLLRQFRDQVG
                     VTPHQYLRIRRVVMAKLHLRAGMPLAEIASACGFFDQSHMTREFKRRTGLTPTAFIPK
                     SPRRGILPQYPRGGALCEEA"
     gene            complement(9713..10531)
                     /locus_tag="VFF98_09885"
     CDS             complement(9713..10531)
                     /locus_tag="VFF98_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZU63981.1"
                     /translation="MRRHLAALLVFSASALAAAALAGEGRPQGYLAPGEFDVTHVLEP
                     APRPGDPRYETDRAIFRQTRQLQGTPRWQMATHDADYATPALLGDFSCALGVRLTVDN
                     APLLVRLVSRAGADTAHETGLAKNVYQRERPFTIDHGPICQPEAELYDSKRQRMSFDY
                     PSGHTTWGWTWALILSQIAPDRAQAILERGRAYGDSRFICGAHNESAVEAGMQSASAT
                     MALVATKPAYQADLAAARAEITALRAHGEKPAGCAAEAALVGQRVMPQLAAPAG"
     gene            complement(10630..11472)
                     /locus_tag="VFF98_09890"
     CDS             complement(10630..11472)
                     /locus_tag="VFF98_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HZU63982.1"
                     /translation="MRAPAPLGLLALLALLALPQGALADPDPASPAGTDQSPGSAEAP
                     PVVHDRAADRFWPAAAMAAAEQAMMAPAPPYAAMKVDLAEYQLGAGADRWRWEGEGWA
                     GDVNRLLLRTRGEGAVGRRLDEAEVEAAYARAISPWWNLEAGVRQDIRPTPARSHAML
                     AAEGMAPYLFDVLAAAYLSDRGQLTGRIEASVDQRLTRRLVLQPRMELNLSAQDMPAE
                     RLGAGLTSAELGLRLRFEITRRFAPYVGAAWTWRSGRTAAYARAEGEGAAARSLVLGV
                     KGWF"
     gene            complement(11469..13142)
                     /locus_tag="VFF98_09895"
     CDS             complement(11469..13142)
                     /locus_tag="VFF98_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HZU63983.1"
                     /translation="MKWPDVARRALLRGGALAGGGVLVGGGARAFSGPPVVSGPPVLS
                     GEAITLRIGRGAVAMGGRMARAVAINGSVPGPVLRLRQGQMLRLTVVNELAEETSLHW
                     HGLELPFQMDGVPGVSFPGIAPGASFTYAFPITQSGTYWYHSHSGLQEMMGCYGALVI
                     DPAEPEPEPVLREHVLLFSDHSFVDPARILARLKADPGYYNHQPQTLADLLAGGKQAQ
                     PLGQRLAWARMRMDPTDIADVTGSVLHYLVNGHDAADNWTGLFTPGEAVRLRCINAAA
                     MTFFNLRIPDLPLEVVAADGQAVQPVVVDEFQFGSGETYDVIVRPGPRAYTIAAETMD
                     RSGMACATLAPAAGMKAAVPPLRPRPLLTMKDMGMAMSGGAMGPMKMRDGANAPQVAL
                     GPGVEMISPMPQDRSGDPGIGLDQAGHRVLTYRDLLAAAPNPDPRAPEREIEVHLTGA
                     MNRYMWSIDGRRMQEAHEPIPMRKGERLRMTLVNDTMMAHPIHLHGHLFELVTGHGTH
                     APRKHTVNVLPGGRISFDMTARPGDWAFHCHLFLHMAMGMMRIVKVRPA"
     gene            complement(13180..13749)
                     /locus_tag="VFF98_09900"
     CDS             complement(13180..13749)
                     /locus_tag="VFF98_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU63984.1"
                     /translation="MADLGELGDGDLAGLALGGRQAAYRVLLDRHRALVFRIARHHCG
                     EEEAALDITQQAFISAFAALGRYDRARPFAHWIARIAINKCRDRARRRRLRAALRLAR
                     PIEEAIGLGDAAPLPDRQVADAEELRRTMAAIARLPARLKEVLVLRAIEGLSQAETAA
                     VLGISEKAVETRLYRARKNLVAQLRDGVG"
     gene            complement(13761..14174)
                     /locus_tag="VFF98_09905"
     CDS             complement(13761..14174)
                     /locus_tag="VFF98_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HZU63985.1"
                     /translation="MVVLGFAAGLGGALAGRALLAPPRPDDTQLHRLLHDQLDLDAGQ
                     AAEIARIEQGFATRKAALEGELRADNADLASAVVAEHGYGPKVAAAVDRSHHAMGELQ
                     KATLEHVFAMRSVLRPDQAARFDAGIVKALTAPAR"
     gene            complement(14207..14473)
                     /locus_tag="VFF98_09910"
     CDS             complement(14207..14473)
                     /locus_tag="VFF98_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63986.1"
                     /translation="MTDLDRLLARLGQLPPDARLAGIDEAVFAGLAAARDPVLPRGAL
                     GAVAGLALLLGALAGGLPAAPAGGALPLGVPGELAPSSLLGDGR"
     gene            14619..14987
                     /locus_tag="VFF98_09915"
     CDS             14619..14987
                     /locus_tag="VFF98_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HZU63987.1"
                     /translation="MRRVLLAALALFASPALAHPAVLSAQPADGASTGPVSQLSITFS
                     EPLVAALSGIDLLMTGMPGMDHHQPMKMTGVKTSLAADGATLVARLPRPLPAGTYEAG
                     WHAASSEAHRTSGKLAFSVK"
     gene            15035..>16179
                     /locus_tag="VFF98_09920"
     CDS             15035..>16179
                     /locus_tag="VFF98_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZU63988.1"
                     /translation="MTPHEHGGCHHHGAATPPETPADAQKVTDPVCGMQVDPLKSPHH
                     AELAGQSWHFCCAGCRSKFLADPDRYLAPAPPAPAEPVPEGTMWICPMDPEVRQDHPG
                     PCPICGMALEPELASADAGPSAEYLDMRRRLLIAAPLALPVFALEMGSHVLPGLHHLI
                     APALSHWVQAVLAAPVVLWAGLPFFRRGWQSLRSGHFNMFTLIALGTGVAFLYSAAAL
                     LVPGAFPADFRDMDGRPAVYFEAAAVIIVLVLLGQVLELSAREKTSGAIRALLDLAPK
                     TARRLTAAGEEDIPLEAVAVGDRLRLRPGESVAVDGVVIEGHSTLDEAMITGEAMPVG
                     KAPGDRVIAGTLNHTGALVIRAEKVGRETMLARIVQLVAEAQRSRAP"
BASE COUNT         2684 a         5679 c         5454 g         2362 t
ORIGIN      
        1 atctggctgc agggctttgc cgaagcgcat gacacgatcg gcggcgttta tgtgggcgtg
       61 cgctatggcc ggcaggaatt caccgacggg cccaacctga tggtctccca gcgcgacaac
      121 aacaacatcc gccaggtgga aaacggcctg cgcctgtggg cccgcggccg caccctgcgc
      181 gccgatctgt tcgacctgca catgaccgcc tatggcatcg atggcaccgg cgacgacctg
      241 ttcgacaaca cccgccgctt ttccggcatg acgctgggct gggtggtgcc gcgcagcctt
      301 ctgggcggct cgaagctcta tctcgatccg ttcctctggc gctatcgcaa cacgctttcc
      361 acctgggggc cgaacaaggg caacgagatg cgcacctatg ccggcgccca cttctggggc
      421 gaggccggcc gcgcgacgat cgactggacg gtgaaccacc agggcggcga ttatgagggc
      481 cgcgccatcg atgcctggca gctgttcctg gcccagtcct tccggctggg ggaaaagccc
      541 tcggcgccgc gcatcggctt ccatgccgat tatgccacag gcggcggcgc ctatggcagc
      601 ggcaagctga aggatgcctt ctcgcccttc ggcaacaaca tctactacag ctacggcctg
      661 ttcctcaccc ccaccaacat gcgcgtgctc tcgcccaaca tcaccttcca gccgctgcgc
      721 aagctgcgga tcaccgggga atatgaatgg gtgtggcgca ataatgcgca ggatgccgtc
      781 tatcgttcca atggcacgcc ctatgccggc acgcaaaagg tgggcgggca cgaggtgggc
      841 cgggtgatgc gcgcgcaggc caccttgtcg atcaccccgc gcctcagctt caccggccgg
      901 gccgaggaga tgtatgccgg cgatgtgctg cgccgggccg gctatgccaa ttccaccttc
      961 cttgccggct ggctctccta tcggttctga cctgccggct ttaagcggca gaggccggat
     1021 gcagggcaca cagggcggtt tcggccagca ccagtgcgcc cgccagcacc agcggcggcg
     1081 ccagcccgcc atgggccacc atgaacgccc ccagcgccgc gcccagaaag atggccagaa
     1141 cgctggccac ccgccggccc agattgggat tgccgccgcc ggccagcgcg ctgtcggcgg
     1201 caaggccggt cagcgtcagg gtcagcacgg tggtggtcag atccggcact ttcagctggc
     1261 ggatggtggc attgcgaaag cccatggcca tggccgtgag ggcgacaatg ccaagcaggc
     1321 ttgccgccgg cgattgcgtg gcgggggtga accccgtggc cagccccgcc gccgcccaca
     1381 gcagccccgc ctccaccagc gccgcgcgca gcagccagcc ccgcagcggc cctgcgccat
     1441 gccgcttgcc tatccgcccg gcccccagcg cgccagccag aaaggcacca atcgccacca
     1501 cataggacag tggatgaaac cccggcgttg ccaccgcggc aaagcccagg aagacgatat
     1561 tgccggtcat gttggcggtg aacaccttgc ccagccccag cacgctgatc gcatcgacaa
     1621 ggcccgtgcc caccgacagc accagcagca ggccgggcaa cagggcagat cggggctcta
     1681 caggcatggt ggtttcctca tggcagatcc tgatggcaga tacgggcatc acatccggaa
     1741 attggcctcg atgccggtca tggtaatggt cttcgcagcg cccgtctcgg ccagaaagcg
     1801 ccccgcctgg aacagcgaga aagaggccga cagcgtcaac tccgccgtgg cgcgccaggc
     1861 cagcgtggcc tcctcctgcg tgccgacgaa acgcgccgcc gccgcattgc ccgcccgcag
     1921 caggtggccc ggcacatcat acacgccatc gccccggctt tgccgccaat aggcgccgcc
     1981 ggaaaggctg gcccccacgc cccgcccgca atccagcgcc agcaccggct gcaggttgac
     2041 gaggttataa ggccccacag gcgacagttc gccgaaatat ttgcccttgg ggaacagcgc
     2101 gttgaacgtg cccagccgat ggtctgaggc ggaacggtcg ccgctggcga tattggcgcg
     2161 cagggtaaaa tcagggtgca gccgcgcccc ggcaaagcgg tggcccacct cgctggcgat
     2221 ggaccaggcg ccgatcgtgc tgtcggcaaa acggccgaac tgggccatgc cttccatgtt
     2281 ccagtgccag cccgccgccc ggcccgtggt ggtgccaaac cagcgcaggc ccagcgtgtg
     2341 gcgcaattcg cggccgcggc cctgctggaa gctggccgcg ctgttgcgat agccgagata
     2401 atagagatcg acgccccccg ccccgaaggc ctgcgtggca taggcccccc acaggcggcg
     2461 cgtgggcgag ctgtgatcgt cgaaactgtt tggccctgcc tccaccggca gcagatagaa
     2521 cgcgttgagc gtgaaggcgc cggtgtggaa gatcgcccgg ccgccatcga aagccagcgg
     2581 caggttgggg ccatagcgcg tgcccaccag ccgctccgtg cccagcccca ccatctcgcg
     2641 cccggcgcgc aggcgcaggc tggcgccgcc ctgcccgccg ccttgcccgc ccagcggcac
     2701 atccacatcg gcaaaggcct gcagcaggtc cgcccgcgtc tggtctatcg cgctgggggc
     2761 cggccgcacg cccaccgcat agcccagcac cggctgcaca aaggcgcgca gctgccccac
     2821 atgcagatcc gcataaggca gcagccgcgc ccacagatag ccattgtctg gcgcgctggc
     2881 ctggccccag aggttcgcgt gataggtctc atcgcgcagg cgcaattcgc ccccagtggt
     2941 gagataggcg ctgcccgtgc catcgagcgg gatatatttg aacgcttccg tccagtgccc
     3001 cgtgcgcccg gcaggatcgg ccaggctcga ccagtcctca ttgtagcgct ccggcgtcag
     3061 caccgggggc tgccagcttt cggccggcgg atcggcccgc gcagcggcag gggccagcag
     3121 ggcaagtgca gcggcaaggg gggcggcaag ggcggggctg gcgcgcatcg ctcaatcgcc
     3181 gcgcccggcc agcagcgcca ccagcacaaa gccgcccacc agcccgccat gctcgaaaaa
     3241 ggcgttggtg gcggcaaagc gctccgcccc cggcggcatc gcccagaaac gattggccac
     3301 cagcgcggcc agcgccgtga acacccccag catccccgcc cccagccaca ccagccggcc
     3361 agacaggacc agcaccgggc cgatcagttc caccgcgatg gtgagcgcgg caaacagcgc
     3421 cggcggcgct aggcccatat gggcctgctc ggccacggca gcgggaaaat cggccagctt
     3481 cgtcagcccg cccagcagat aggcaccgca cagcgccagc cgcgcaagga agcccgtgcc
     3541 ccgccagccc agcagggccg ccaccgggcg cggcggggca ggctggctca ttgcccggcc
     3601 gccccgtgga tctcggcggc gtggatttcg gcgatatagc cgccggggaa gcgcacaatg
     3661 gcgctgtgcc gcccggcgcc gatatgggcc ggcaccagca cctccacgcc ggccgccgct
     3721 gcccttgcca gcgtggcggg cagatcggcc accgcatagc cggtgagatc gcgcccataa
     3781 ggccagggca actggccatc ggaaacgatc accagcatcc tgccatagcc gctctcgatg
     3841 gcgatctggc ggatggtctt gccgggctgg ccgatcgcgt caccggcaag gccgcgctca
     3901 tcggcgacaa ccttgccatg ggcgaaggcc agccagtcct tcacgaagcc atcggcggca
     3961 tcggcggtga ggtagatgcg gttttccggc acgctggcca gcggcgcata gctgggcttg
     4021 gcggtgtgcc agtagagctg catgttcacc ccgcccggcc actggatcac cacatcgcgc
     4081 ccgatcggat cgggaaaggt ggtgatcagc cggctggcgc catgctgcac ggcggcggcc
     4141 accgcgccat ccatgtcgct caccagatag ccggtgcgct cggcgccgaa gggatagggg
     4201 atctcggtct tgaagccgaa caccgagacg gtgccgaccg ggctgagcac cagctgcgat
     4261 ttggtgaggc tgggcgtggg cgttacctgg aattcgccct gcttgctggc ggtgccgccg
     4321 aaagtggcga ggaagctggc cacgaaacgg tcgaaatcgc tggccggcac atagacatgg
     4381 gtcgtgtcat attgcgggcc cacggcataa tcgggggtgg cggcaggggc ggccatggcc
     4441 gggctggcca gcgccagggc aaggaagagg gcaaggcgtt tcatcggata tctccagacg
     4501 gaaaaaggtt ggctcaaaca gcccagcagc cgcagcccag cgcgccccag aagctctgcg
     4561 gatcggcagt gggggtttgc gcgccaaggg cggcggcatg gccatggccg tgcaccgcgc
     4621 aaggcaccgc gcaggcacag gccgacgcca gcttccagcg cgtttcgggc gattgccaat
     4681 ggccgccaaa cgtgcccacc ggcgaccagt cgggcattgg ccggggcaga tcaggggcat
     4741 gggcggcata atcgccattg ccatgcacca ccttgccgcc cagaatggtg agcaccgagc
     4801 ggatctggct gatcgcgctt tccggcacgg cgaaataatc gtcagacagc agcgccagat
     4861 cggccagctg gccagccttg atctggccct tccggcccac ctcgttcgag aaccaggtgt
     4921 tggcctcggt ccacaggcgc agggccgtct cgcggtcgag ccggttgcgc accggcgata
     4981 gcgccagccc gcccaccgtg cggctggtga ccagccatga cagcgacacc caggggttgt
     5041 agctggccac gcgcgtggca tcggtgccgg cgccgacggg gatgccggct tccatcatgc
     5101 gcttgatcgg cggcgttgcc tcggcggcct tggcgccata gcgttcgacg aaatattcgc
     5161 cctggaaggc catgcgatgc tgcacggcaa tgccgccgcc aagcgccgcg atgcgatcga
     5221 tgttgcggtg gctgattgtc tcggcatggt cgaagaacca gttgaggccg tggagcggga
     5281 tatcgcggtt gaccttctcg aagacatcga gcgcgcggct gatcgtctcg tcataggtgg
     5341 cgtgcaggcg ccagggccag cggcgttcgg ccagcaggcg gatgacgggt tcgagatcac
     5401 cttccatcgt cggcggcata tccgggcgcg cttgccgaaa atcctcgaaa tcggcggcgg
     5461 aatagaccag catctcgccc gcgccattgt ggcgataggt atcgtcgcca tcgccgggct
     5521 tgacctgctt cgaccagttg gcaaaatcct ccagttcctc tttcggcttt tgcgtgaaga
     5581 ggttgtaggc aatgcgcagg gtcagcagcc catcggcatg cagcttttcg atcacctcgt
     5641 aatcatcggg atagttctgc gagccgccgc cggcatcgat cacgctggtc acgccaaggc
     5701 tgttcagctc gcgcatgaac tggcgggtgg aattgagctg gtattcctgc ggcagcttgg
     5761 ggcccttggc gagggttgcg taaaggatcg tggcgttggg ctgggccagc agcaggccgg
     5821 tggggttgcc ggctgcatcg cgcatgatct cgccgcccgg cgggttgggc gtgtccttcg
     5881 tgtagcccac ggcgcgcagg gccgcggcat tcagcagcgc gcgatcatag aggtggagga
     5941 ggaaaaccgg cgtttcgggg gcggccgcgt tgatctcgtc gatcgttggc aagcgctttt
     6001 cggcaaactg gtgctcggta aagccgccca ccacgcgcac ccattgcggc ggcggcgtca
     6061 cctcggcctg gcgcttcagc atggccatcg cctcggcaag gctgggcacg ccttcccagc
     6121 gcagttccat attgtagttg aggccgccgc ggatgacatg gatgtggctg tcgatcaggc
     6181 ccgggatcag gcggcggccg ccggcatcga tgatctgcgc ttgcggcacg gccgcgcggg
     6241 catccgcttc agagcccgcc accagaatgc ggccatcgcg gatggccacg gcctctgcct
     6301 gcgggttggc gcgatcgagc gtggtgacgc gcgcgttgat gaggagaagg tcggtcatcg
     6361 ggggcactcc ggttcaggcg tcgcgctggg gggtagtttg gtggggggca gcttggtggg
     6421 gggcggcgtg ttcgcccagc acgcggcggg cgcagtcggt cttgacgaag gccagcaccg
     6481 gctgcccgcg cagcaggcgg gccgccaccg gcaccgcctg ctcgcccagc agcatgccca
     6541 gaaggccgat cagcgcgata gtaggcggcg ccggagacct gacccccagc agcgcataga
     6601 tggcgccaac cagcacgccg gccgccagcg aaatcagata gggtttcatg tctgggcctt
     6661 tcggaaggaa aaggggcgcc gggcccggat gggggggccc ggatcgggag gtccgggagg
     6721 aggccggccc ggcgccagtt gcctcagcgc atcagcctcg gcgcatcagt ggccgccttc
     6781 ggaggcgccg aacatcgtct tggcgtaggt gatgccaagg ccataggcgc cgcccagctc
     6841 acgcgcgagg ccggtggtga aggcataggt ttcgccgcgg gcccagtcgc gctgcagttc
     6901 cagcagatat tgcagcgccg tcatcgggtg ggcgccggcc tggatcatgc gctccaccgc
     6961 gcgctcatgc gcctcggccg agatgtcgcc gcaggcatcg gtgatgacat agacctcata
     7021 gccctgctcg atggccgaca gcgtggggcc aacgatgcac accgaggtcc acaggccggc
     7081 gaacaccagc cggccaaggc cgatgcggtt cacttcctcg atcaccgcgg catcttccca
     7141 ggtgttcatg ctggtgcgat cgagcagggg ctggccgggg aacgggtcgg tcacctcgct
     7201 gaacatcggg ccggaaaagc tcttctcggc caccgtcgtc aggatcgtcg gcacgccaaa
     7261 accggcggca gcgccagcga tcaggccggc gttgttgcgc agcagggtgg tgtcgatgga
     7321 cttggtggca aaggccatct gcgactggaa atcgatcagc accagcgcat ggttgtcggg
     7381 ggcgatcagg gccttggcgg gcgtgggggt ggcagtaagg gtcatgtcaa actccatcgg
     7441 tgaagggggc gcgcgccatc cgccgggccc ggcagcctgc cggccctttg gccaaacgcg
     7501 gtgaacacgg gggaaatcag ctgcggaaga aggccagcag atcggcgttg atgagatccg
     7561 gatgggtgct ggccatcccg tgcgagaggc cgggataggt tttcagcgtg ccctgctgca
     7621 ccagctgcac gctcaaccgg gcagaggcag ccagcggcac cacctggtca tcctcgccat
     7681 ggaggatcag cacgggcaca tcgatgacct gcagatccgc ggtgaagtcg gtttccgaga
     7741 aggccttgat gcaatcgtaa tgggccttgg cgccgcccat catgccctgg cgccaccagt
     7801 tctcgatcag gcctggcgac acggcggcat ccgggcggtt gaagccatag aaggggccgc
     7861 tggccacatc gaggaagaat tgcgcgcgat tggccgccag cgcggcgcgg aacccatcga
     7921 acacggccag cggcaggccg gcgggatggc tgggcgattg cagcatgatc ggcggcaccg
     7981 cgccaatcag cacgatccgc gccacccggc cgggctctgc gcgggcggca taatgggcca
     8041 cctcgccgcc gccggtggaa tggccgacat ggatcgcccc gcgcagatcg agatggcgcg
     8101 ccagcgcggc cacatcggcg gcataggtgt ccatgtcgtt gcccgtgtcg gtctggcccg
     8161 atcggccatg gccgcgccgg tctggcgcga tgacgcgata gccctgctgc aggaagaaca
     8221 gcatctgggc atcccattcg tcgcccgtca gcggccagcc atggtggaag acgataggcg
     8281 gcgcggcgct gtcgccccaa tccttgtaga acagctccac cccatcggca gtgacaagcc
     8341 gggcgctgcc gggcctggaa agcgtatctg tcatgaaaaa tcccgcctgt cgcaaagcgt
     8401 ttgcccacaa tgacgcagcc gggcagaggg acacccctca aaacggtcgc agaaatttat
     8461 ctccgcgatt gcgaagtgca aagaatttct gcaacgctct ttccggcttt ctgtcaacgc
     8521 caacctatgc aaggcgcacc ctggcgtatt ggacgggatt gaccgcagcg aagattatca
     8581 cgcctcttcg cacagggcgc cgccgcgcgg atattggggc aggatgccac ggcgcgggct
     8641 tttcgggatg aaggcggtgg gcgtgaggcc agtgcggcgc ttgaactcgc gggtcatgtg
     8701 gctctggtcg aaaaagccgc aggcgctggc gatttcggcc agtggcatgc ccgcccgcaa
     8761 atgcagcttg gccatcacca cgcggcggat gcgcagatac tggtgcggcg tcacccccac
     8821 ctgatcgcga aactggcgca gcagcacgaa gcggttcatc ccggccaccc cggccagatc
     8881 ctccagcgcc acattctcgg cataatgcgt gcgcagatag tccaccaccc gggcgatgcc
     8941 gcgctggcag gcgcccatgc gcgcctgccc cgccgcgggc gacagccgcg cggccagttg
     9001 ctccatccag gcggcaatgc cctcgaaggc ctccagctcc tcgtggttct gctcgaaacg
     9061 ggcgtgcagc gccagcaact ggcgcgacaa cgccccatcc gccagtagcc catcgccgaa
     9121 atccagccgg gccacagggc ttgcctccag ctcggcccat tcggccaggc gccggtgcga
     9181 gaggtggatc tcgcggcaca cgccgccggt ctgcgcgatc cattccgagc ggatggcgaa
     9241 atgctcgccc gcgcgccgca tgatgatatc gcccggccgc tcctgccgcg cgccatcgcc
     9301 attgcgcgac caccagccgc agccctcgaa ggcgaggaag acgatatcgt cgtggatcat
     9361 ctcgtcgata tcgagctggg cgccgccagc cagatcgaag cgcacgaggc tgcaatcggc
     9421 cccgccccac acattcatcg tatgacgggt agtggaaaca tgatccatcg cggggcatcc
     9481 tgtgggaaga ggcaggtttg gggaggaaga cttgcggggc aggcgcgccg cgcgaggaac
     9541 agcgcgaggc catcaagact atcagccctg caaggccatg cgaatgctac caatgcgcaa
     9601 tagctgcaac cgcaaaaagc cgcctcggcc gattccccgc gcgccgcctg ccccgtgata
     9661 ccaacccgtg ataccaagtg gtaacacgag ggcggcgttt gccaaagccg gatcagccgg
     9721 cgggcgcggc cagttgcggc atcacccgct ggcccaccag cgccgcttct gccgcgcagc
     9781 cggcgggctt ttcgccatgg gcgcgcaagg cagtgatttc cgcccgggcc gcggccagat
     9841 ccgcctgata ggccggcttg gtggcaacca gcgccatggt ggcgctggcc gattgcatgc
     9901 ccgcttccac cgcgctttca ttatgcgcgc cgcagatgaa gcggctgtcg ccataagcgc
     9961 ggccgcgttc gaggatcgcc tgcgcgcggt ccggcgcgat ctggctgagg atcagggccc
    10021 aggtccagcc ccaggtggtg tggcccgatg gatagtcgaa gctcatgcgc tggcgcttgc
    10081 tgtcgtagag ttcggcctcg ggctggcaga tcgggccgtg gtcgatcgtg aagggccgct
    10141 cgcgctggta gacgttcttg gcaaggccgg tttcatgggc ggtgtcggcc ccggcccggc
    10201 tgaccaggcg caccagcagc ggcgcgttgt ccaccgtcag ccgcacaccc aacgcgcagc
    10261 tgaaatcgcc cagcagcgcc ggcgttgcat aatcggcatc atgggtggcc atctgccagc
    10321 ggggcgtgcc ctgcagctgg cgggtctggc ggaagatcgc ccggtcggtc tcgtagcgcg
    10381 gatcgccggg gcgcggggcg ggctccagca catgggtcac atcgaactcg cccggcgcca
    10441 gatagccttg cgggcgcccc tctccggcga gggcagccgc cgccagcgcg ctggcggaaa
    10501 agacaagcag ggcagcaaga tggcggcgca tgggcaggcg tggtcctttc ggcagctggc
    10561 ggcagcagcg aatcccgcgc cgccccgcct tatcagccaa ggccaagctt cagcgcaagc
    10621 cggcccgccc tagaaccagc ccttcacgcc cagcaccagg ctgcgcgcgg ccgccccctc
    10681 gccctcggcg cgggcatagg cggcggtgcg cccgctgcgc catgtccagg cggcgcccac
    10741 atagggggca aagcggcgag tgatctcaaa gcgcaggcgc aggcccagct cggcgctggt
    10801 caaccccgcg cccagccgct ctgccggcat gtcctgcgcc gacaggttca gctccatgcg
    10861 cggctgcagc accagccggc gcgtgaggcg ctggtcgacg ctggcctcga tgcgcccggt
    10921 cagctgcccc ctgtccgaca gataggccgc cgccaacacg tcgaagagat agggcgccat
    10981 gccctctgcc gccagcatcg catggctgcg cgccggagtg gggcggatat cctgccgcac
    11041 cccggcctcc aggttccacc acgggctgat ggcgcgggca taggcggctt ccacctcggc
    11101 ctcatccaac cggcgcccca ccgccccctc gccccttgtg cgcagcagca gccggttcac
    11161 gtcacccgcc cagccctcgc cctcccagcg ccagcgatcg gccccggcgc ccagctgata
    11221 ttcggccaga tccaccttca tcgcggcata gggcggggcg ggcgccatca tcgcctgttc
    11281 ggccgcggcc atggcagcgg cgggccagaa gcggtcggcc gcgcgatcgt gcaccacagg
    11341 cggggcctcg gcactgccgg gcgactggtc ggtgccggca ggggaggccg gatcaggatc
    11401 cgccagcgct ccttgcggca acgccaacag ggccaacagg gccagcaggc caagcggagc
    11461 cggcgccctc atgccgggcg caccttgacg atgcgcatca tccccatcgc catatgaagg
    11521 aagagatggc agtggaaggc ccagtccccg ggccgcgcgg tcatgtcgaa gctgatcctg
    11581 ccgccgggca gcacgttcac cgtgtgcttg cggggcgcat gggtgccatg gccggtcacc
    11641 agttcgaaga gatggccgtg gaggtggatg ggatgggcca tcatcgtgtc gttcaccagc
    11701 gtcatgcgca ggcgctcgcc cttgcgcatg gggatgggtt cgtgcgcctc ctgcatcctg
    11761 cgcccgtcga tcgaccacat gtagcggttc atcgcgccgg tcagatgcac ctcgatctcg
    11821 cgttcgggcg cgcgcggatc ggggttgggc gcggcggcca gcagatcgcg ataggtgagc
    11881 acccggtggc cggcctggtc gagcccgatg ccgggatcgc cgctgcggtc ctgcggcatc
    11941 gggctgatca tctccacccc cggccccagc gccacctgcg gggcattggc gccatcgcgc
    12001 atcttcatcg gccccatcgc cccgccagac atggccatcc ccatgtcctt catggtcagc
    12061 agcgggcgcg ggcgcagcgg cggcacggcg gccttcatgc cggccgcagg cgccagcgtg
    12121 gcacaggcca tgcccgaacg gtccatcgtc tcggcagcga tggtgtaggc gcgcggaccc
    12181 ggccgcacga tcacgtcata ggtctcgccg gagccgaact ggaactcatc gaccaccacc
    12241 ggctgcaccg cctgcccgtc ggcggccacc acttccagcg gcagatcagg gatgcgcagg
    12301 ttgaagaagg tcatcgccgc ggcgttgata cagcgcaggc gcaccgcctc gccgggagtg
    12361 aacaggccgg tccagttatc ggccgcatca tgcccgttca ccagataatg cagcacgctg
    12421 ccggtcacat cggcaatatc ggtcgggtcc atgcgcatgc gcgcccaggc caggcgctgg
    12481 cccagcggct gggcctgctt acccccggcg agaagatcgg ccagcgtctg cggctggtgg
    12541 ttgtagtagc cgggatcggc cttcagccgg gcgagaatgc gcgccggatc gacaaagctg
    12601 tgatcggaga acagcagcac atgctcgcgc agcaccggtt ccggctctgg ctctgccgga
    12661 tcgatcacca gcgcgccata gcagcccatc atctcctgca ggcccgaatg gctgtgatac
    12721 cagtatgtgc cgctctgggt aatcgggaag gcataggtga agctggcgcc cggagcgatg
    12781 ccgggaaagg agacgcccgg cacgccatcc atctggaagg gcaattccag cccgtgccag
    12841 tgcagcgaag tctcctcggc cagctcgttg acaacggtga ggcgcagcat ctgcccctgc
    12901 cgcagccgca gcaccgggcc cggcacagag ccgttgatcg ccaccgcccg ggccatccgc
    12961 ccgcccatcg ccacagcgcc cctgccgatg cgcagggtga tcgcttcccc cgaaagcacc
    13021 ggtgggccgg aaaccacggg cgggcccgaa aacgcgcgcg cgccgccccc caccagcacc
    13081 ccgccgccgg ccagcgcccc tccgcgcagc agcgcccggc gcgcaacatc aggccatttc
    13141 atgacaaccc ccacagcaac atctgccagc ctatacgcct cagcccaccc catccctcag
    13201 ctgcgcgacg agatttttgc gcgcgcggta gagccgcgtt tccaccgcct tttcgctgat
    13261 gcccagcaca gcggcggttt cggcctggct caacccctcg atcgcgcgca gcaccagcac
    13321 ttccttcagc cgcgccggca agcgggcaat cgccgccatg gtgcggcgca gctcctcggc
    13381 atcggccacc tggcggtcgg gcagcggggc ggcatcgccc agcccgatgg cctcctcgat
    13441 gggccgggcc aggcgcaggg ccgcgcgcag ccggcgccgg cgcgcccgat cgcggcattt
    13501 gttgatggcg atgcgggcga tccaatgggc aaagggccgc gcccggtcat agcggccaag
    13561 ggcggcaaag gcgctgatga aggcctgctg ggtgatgtcg agcgcggcct cttcctcgcc
    13621 gcaatggtgg cgggcaatgc ggaacaccag cgcccggtgc cgatcgagca gcacgcgata
    13681 ggccgcctgc cgcccgccca gcgccagccc ggccagatcg ccgtcgccca attcgcccag
    13741 atctgccacg ccggtgcggc ctagcgggcc ggcgcggtga gggccttgac gatccctgca
    13801 tcaaaacgcg ccgcctgatc gggccgcagc accgaacgca tcgcaaagac atgctccagc
    13861 gtggccttct gcagctcgcc catggcatgg tgcgagcgat cgaccgcggc ggccactttc
    13921 ggtccatagc catgttcggc cacgacagcg ctggccagat ccgcgttatc ggcgcgcaat
    13981 tcgccctcca gcgcggcttt gcgggtggca aagccctgct cgatccgggc gatctcggcc
    14041 gcctgcccgg catcaaggtc gagctgatcg tgcagcaggc ggtgcagctg ggtatcgtcg
    14101 gggcggggcg gggccagcag cgcccggccg gccagcgcgc cgcccagccc ggcggcaaag
    14161 cccagcacca ccacccacgc cagcgccccc ttgcgccacc gcgcgctcac cggccatcgc
    14221 ccagcaggct ggaaggggca agctcgcccg gcacgcccag cggcagcgcg ccgccggcag
    14281 gtgccgccgg cagcccgccc gccagcgccc ccagcagcag cgccagcccc gccaccgcgc
    14341 ccagcgcccc gcgcggcagc accggatcgc gcgcggccgc cagcccggca aacaccgcct
    14401 cgtcgatgcc ggcaaggcgg gcatcggggg gcagctggcc aaggcgggca agcaggcggt
    14461 caagatcggt catggcagca tccttcggca atccgcccca gcatacgcgc ttcctgcgcc
    14521 attccctcat gccgccggca aaaaaagcgc accctcgcgt gagggggccg cccgcccgct
    14581 gcgtaaacac cgccaaacca tagcggagtt tttgccccat gcgccgtgtt ctgctggctg
    14641 cccttgccct gtttgcttct cctgcgctgg cccatccggc ggtgctgtcc gcccagccgg
    14701 ccgatggcgc cagcaccggg cccgtctcgc aactgtccat caccttcagc gagccgctgg
    14761 tggccgccct ctccggcatc gacctgctga tgaccggcat gcccggcatg gaccatcacc
    14821 agccgatgaa gatgaccggc gtgaaaacca gccttgccgc cgacggcgcc acgctggtgg
    14881 caaggctgcc ccgcccgctg cccgccggca cctacgaggc cggctggcac gcggcttcct
    14941 ccgaggccca caggacaagc ggaaagctgg cgttttccgt caaataaggc aggatgacgc
    15001 cgccctcccc ccggcgcagc gaaggacccg atcgatgacc ccgcacgagc atggcggctg
    15061 ccaccaccac ggcgccgcca ccccgcccga gacgcctgcc gatgcgcaaa aggtgacaga
    15121 ccctgtctgc ggcatgcagg tcgatccgct gaaaagcccg catcatgccg aactggcggg
    15181 gcaatcctgg cacttctgct gcgccggctg ccgcagcaaa ttcctggcag accccgatcg
    15241 ctatctcgcg ccggcgccgc ccgccccggc agagcccgtg cccgaaggca ccatgtggat
    15301 ctgcccgatg gacccggaag tgcggcagga tcatcccggc ccctgcccca tctgcggcat
    15361 ggcgctggag cccgaactgg ccagcgccga tgccggcccc agcgccgaat atctcgacat
    15421 gcgccgccgc ctgctcattg ccgcgccgct ggcgctgccg gtgttcgcgc tggaaatggg
    15481 cagccatgtg ctgccgggcc tgcatcacct catcgccccg gcgctgtcgc actgggtgca
    15541 ggcggtgctg gccgcgccgg tagtgctgtg ggccgggctg ccgttcttcc ggcggggctg
    15601 gcaatcgctg cgcagcgggc atttcaacat gttcacgctg atcgccctcg gcaccggtgt
    15661 ggccttcctc tacagcgcgg cggcgctgct ggtgccgggc gccttcccgg ccgatttccg
    15721 tgacatggat gggcgcccgg cggtctattt cgaggcggcg gcggtgatca tcgtgctggt
    15781 gctgctgggc caggtgctgg aactgtcggc gcgggagaaa acctcgggcg ccatccgcgc
    15841 cctgctcgat cttgccccca aaaccgcccg ccgcctcacc gccgctggcg aggaggacat
    15901 ccccctcgag gcagtggccg tgggcgaccg gctgcgcctg cgcccgggcg agagcgtggc
    15961 ggtggatggc gtggtgatcg agggccattc caccctcgac gaggcgatga tcaccggcga
    16021 ggcgatgccg gtgggcaagg cgccgggcga ccgggtgatc gccggcacgc tcaaccacac
    16081 cggcgcgctg gtgatccgcg ccgagaaggt gggccgcgag acgatgctgg cccgcatcgt
    16141 gcaactggtg gcagaagccc agcgcagccg cgcgccgat
//
LOCUS       DASRYE010000190        26177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_82208,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000190 DASRYE010000000
VERSION     DASRYE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 26177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26177
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82208"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFF98_09925"
     CDS             <1..258
                     /locus_tag="VFF98_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63989.1"
                     /translation="RTVVALALRDPLWSVPRPRRWFRPGPPRAERLQLANPRLESLRR
                     FVILRRHGQDSGDGATTLLNCGFSPGQIREAGRLADLHSGA"
     gene            354..1136
                     /locus_tag="VFF98_09930"
     CDS             354..1136
                     /locus_tag="VFF98_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZU63990.1"
                     /translation="MKIYTITACAPLALLTACPAPAQEIDAAAQTDAAHNDDHVILGM
                     GALYAPAHEGGDTYRALPMPAIDIKEGRFFANLRDGVGANLIDTDLLTLGASLTYTQG
                     YRAKDVPAGVGAVKLGAGARGFLSLHDQGLILTLGMTQGFAGGTKGLIADASLSYPIA
                     ATRRLTLIPTIGTSWANAKFNNRYFGIDAAQASASGLNTYAPDAGFKDASGILTASYQ
                     LTDHILLSASGVVTRVLGADANSPLVAHKTQPSGFLSVAYRF"
     gene            complement(1146..2132)
                     /locus_tag="VFF98_09935"
     CDS             complement(1146..2132)
                     /locus_tag="VFF98_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU63991.1"
                     /translation="MLRIEKRIDVSAAAPELGEPIEMAVTLSLPDPASLPERPVAVFA
                     CPGGGYSRHYYLMRFDGHADYDEGAWQAGRGVIHVAIDHVGVGESTIPDLARISFQTL
                     AATHDACVRAITASFLDGTAADGYPALPSLFKVGMGQSMGGGVTLLAQGRFATFDAIA
                     PFGVSAIHTALPQRDAGAFAQGVRRFDAVAQGKVTSHIEHDHQGVDYLYAFHWEDVPP
                     DILHEDMQGGYPIRQTSPRFGSLTIPHCAVQMMLPGCFTADAASVTVPVLIGIGERDT
                     CPEPYREPAAYAGSPDVSLFIVPEMAHMHNFASTRQILWQRLHGWARLVAGA"
     gene            complement(2508..3989)
                     /locus_tag="VFF98_09940"
     CDS             complement(2508..3989)
                     /locus_tag="VFF98_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU63992.1"
                     /translation="MNTARSLRAAGCALLTAASAVLAGCAVGPDFRAPPAPAGKAYDP
                     QPIAAAAAPLAGGGQALSPTAPLPAEWWRPFHSPALDLLVAEALARNSDLAATRAALK
                     AARESWLASKGVLLPSVDAGASTSRAKGTQYLAPALADNSFDYNLQTAQVTVGYTLDL
                     FGANRRQVEQARAQYQAQRFEAEAARLTLINTVIATALTEASLHLQVAAQERIIELAL
                     AQRDIARRQQDQGEIAGAALMAQEGALAQARAALPPLRKQWTQARDALAYLTGRAPAE
                     GPPASVDLAEVRLPRDLPLSLPAELVRQRPDLRAAEENLHAASAGVGIAIAARLPQIT
                     LSASGGGSSPGWASLLSGANSFWSLGAGVSQPLFAGGALLHKQRAAKATLDQARELYR
                     SAVLAAFQNVGDTLHALQEDATALDAAEQARQAAQGQYEVACRQARLGEIPEMTRLAA
                     EQALRQADQAEAAAAAQRLADTAALYQALGGGWQGADGAKAER"
     gene            complement(3986..7189)
                     /locus_tag="VFF98_09945"
     CDS             complement(3986..7189)
                     /locus_tag="VFF98_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU63993.1"
                     /translation="MNPIVAFALKQRLMILALLGLFLLGGLFAFRQLNIEAYPDPVPP
                     LVEVVTQSPGLSAGEIERTITVPVEVQLAGIKNVKKVETISLFGLSDVRVQFTYDYGY
                     EQAEQKVINALSQLPPLPGGAQAGISPESPIGEIYRYRIVGPKGYSVTDLKTIQDWIL
                     QRRFKAVPGVVDVNGWAGKSKTYEVTIDQRRLVAYGLSIGQVLSALNNANANVGGQTV
                     NLGPQSAVVRGVGLIHSMDDIRDTMIASVSGKPVQVKDVASVEVGNQPRLGIVGQDND
                     DDIVQGIILMRRGEESLPTLQRVEAEVAKINAEGVLPPGVHLEKIYDRTDLIDVTTHT
                     VLENMIFGVVLIFLVQWIFLGTLRSALIVAATIPFALAFAIIIMVLHGESANLLSVGA
                     IDFGLIVDATVIMVENIFRHLAQPRRDRFHKGGTLDRKGGIQSFFGKLGTIELSAAEV
                     NRAIFFAAAIIIAGFLPLFTLTGVEGHIFGPMARTYAYAIIGGLLATFTVAPVLSAVL
                     LKEQEEETETPIVRWMRRLYTPAIRFALANRVVALGGMGALLLGAVLASSSLGLEFLP
                     KLEEGNFWIRATLPPTVSLEEAQGYVNRMRHILMKYPEVKTVVSQLGRPDDGTDTTGF
                     FNAEFYVPLKPGNTWPWGMTKEKLTREVNADLAAPFPGVEFNFSQNIEDNVEEAASGV
                     KGQNSVKLYGNDLEALEKTAYQIKAVMAGVPGITDLSVFDALGQPTINIKIDRNRAAR
                     YGLAPADVNAVVQAAIGGQAAGNLYEYGSDRNFPIIIRMAPQFRGSLDAIRAIPVAVA
                     NASGGTSQIPLSTIADVQLVSGYSFIYRENQTRYIPIKFSVRGRDLGGAVLEAQARID
                     RQVALPAGARLEWVGEFGELQDALSRLEIVVPISLLLIALLLFINFGSLTDTLLAASV
                     MPMALVGGIYALFFTGTAFSVSAAIGFIGLFGISVMEGILVLAYYNQMLGVGMGRSEA
                     ILHAAETRFRPVMMTCIAACVGLLPAAVSTGIGSQVQKPLALVVVGGILLAPVLILVV
                     LPVMIEMFSRRQPQKVDIDEDDVAMPGAAPAKDGPSA"
     gene            complement(7196..8389)
                     /locus_tag="VFF98_09950"
     CDS             complement(7196..8389)
                     /locus_tag="VFF98_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU63994.1"
                     /translation="MTDLPAPTHSPSGPQLNRRQQIRLAGAAVVASLLVFGAAPRLWS
                     LMAPPPAAPAPAPADGSFAATDRQWQTLRFETLRSQTWQNGVVTDGKIAVDDDRTTQV
                     LSPFTGRVTRVFAKVGDRVRAGDPLFAVIANEAAQNDADMLTGVATLKAAEAEAARMR
                     DLSRHQGAAEKDLEAAEVNLASARAALNAAEARRLALGGSLSRGEGVVRAPVAGIVTQ
                     RLIGLGQNVASASGGAATQAFTISDFGQVWVVGNLREEDAAKAHVGQLAEVRLLVDPD
                     HPLAARVDYVASALDPASRRLTVRASLANAGGQLKPEMFASFRLLTGGARTVLTAPEA
                     AVIYEGATARVWVAQPSGHRLALRAIGTGATFDGRVEITAGLQAGETVVTAGSLFIDR
                     GAKAD"
     gene            complement(8452..9825)
                     /locus_tag="VFF98_09955"
     CDS             complement(8452..9825)
                     /locus_tag="VFF98_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU63995.1"
                     /translation="MIRPFRPIARSMLLRILLIEALTFLAACIALPLLTLSELRSSAE
                     RIQAEMLMGQAQTLRGGLAYDARRGWTLQPGDTVRATFDSGYDGRAYALMDGAGHPVS
                     ISRYAQPRAWPGRIYAARAMRFEAGPLTGLSLPVAVAGQRGWLVVTQDQGRPGAVVDD
                     VMRAFLARYLVALIGLLMLMLAINGLLLWHTFRGLRAITRQAAEIGPRAFSLQLGEQG
                     LPSEIRPLVQAINGLLARVHQALHQQDEFAGNVAHELRTPLAALRLRAEQVVEPPLRQ
                     AMALQVERMGHVLSQLRDLAALEGAAAASMAPVDLGDLAVGVVAELSPRILAAGRTIE
                     LRGEGMSVLGNAGLLAIALANLIENAAVHTPVGTHITVALEGAGVLAVIDNGHGILEE
                     DGPRLLRRFHRADHARTDGAGLGLSIVQRIADAHRARLEAGPAAAKGARFAIRFGDAA
                     VHGQGAP"
     gene            complement(9842..10525)
                     /locus_tag="VFF98_09960"
     CDS             complement(9842..10525)
                     /locus_tag="VFF98_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU63996.1"
                     /translation="MRLLLVEDDRALALAVSDHLRQNGYGVDLAGKGEDAALLLATSQ
                     YAAMILDRGLPDGDGLDLLRGLRRQGHNVPVIMLTARSEIASRIAGLDAGADDYLAKP
                     FSPDELVARLRAILRRNGTFLGPVAHFANMAFDLDALSLQVDGVLVNLSAREASLLAL
                     LLRRSGQVLTRRLAEDQLFGGDGLGSNAIEVYVHRLRHKLSAAGARVEVVTIRGVGYL
                     LSDLSQGAA"
     gene            complement(10620..12032)
                     /locus_tag="VFF98_09965"
     CDS             complement(10620..12032)
                     /locus_tag="VFF98_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU63997.1"
                     /translation="MTEYTLLIDGAAVATTASDPVIDPASGAVFAHAPQAGTAELERA
                     VAAARAAFPAWAADEPLRRQVLARAAERLRERAGPIAQLITREQGRPLAQALGEVQYG
                     AAILDSYAAFTVPDEVLAERPGAAPVQVVRAPLGVVAAITPWNVPVLLLLRDIVPALL
                     AGNTVVAKPSEHTPLSSLALGRALADIFPAGVLNILAGGKELGAELTRHPDVAKVTFT
                     GSVRGGKAVAAQTAGDLKRVSLELGGNDAAIVLADAPVEKVARALFEAAFFNAGQICF
                     AVKRVYAARAVFAPLVAALAALADAARPGPGLDPASTLGPLTTREQFDRVRALVADAL
                     AHGAVLHQGQAPLPAEGYFHAPTIVTGIGKGVALVDEEQFGPVLPVIPFDDVEEAIAA
                     ANDTPFGLGGSVWSADVAQAARIVQRLDVGYGWVNQHARPFPAAPGGGHKWSGIGYRG
                     GRIGYDEYVALQVVDAVPLA"
     gene            complement(12055..13671)
                     /locus_tag="VFF98_09970"
     CDS             complement(12055..13671)
                     /locus_tag="VFF98_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZU63998.1"
                     /translation="MAPPFSFPAGPFDVIIVGGGSAGSVLAGRLSADPALSVLLIEAG
                     PDLTPDTAPPEILDASDASGPLRSGARFMWPDFYTTRHADQPPGRYYEQARILGGGSS
                     INMQAANRGVPSDYDDWAARGAEGWHWEAVLPYFRKLETDTTFGEGPLHGGSGPIPIS
                     HDRLEHWSGFNRAVSAVLADHGLPFLEDQNGDFRTGHFRMAQSHDGQQRSSAAFGYLD
                     ATVRARANLTLLTDTTVSALLVEGGAVRGVDIGTPAAPRPLAARRVILAAGALHTPVL
                     LLRAGIGPADELRALGIAPVADRPGVGRGLWEHASIALVSPVAGGQWPQGWRRSGVGA
                     RFSSGLPGADPADLFAVITASPEGMMAGLWINRPDSVGRVALSGPGLADRPLRDLNLL
                     ADPRDIDRLRIGLRRLAAVLGDARLAPFGLAPQLYRFGVPEGGISAPLAELLADDAAL
                     VRHLRYNVSGVWHACGTARMGRAEDPLAVVDPEGRVYGVAGLWVGDASVFPGVPSANI
                     NLPTLMAAEKLADAFLARHALAPASAALPA"
     gene            complement(13673..14956)
                     /locus_tag="VFF98_09975"
     CDS             complement(13673..14956)
                     /locus_tag="VFF98_09975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU63999.1"
                     /translation="MTTDTSLPADGPQPFLSRLPTTRAGSWPAILAIYAYAELIAACH
                     GKTLLLVPDVARSFAVDPARASWVIAAVALVAAVGAPFGGWLIDRLGEHRAIRLGLAL
                     AAPSSLAAAFVPRFAELVLLRVIEGAGYIAVVLGCLSLLIRTTSGPRQTTALAFWSVA
                     SPMGGALAILAVSPLAGTAQWRWVFAGHAGLLLLALVASPLLPAAPPRARASERGGAS
                     PWAVYRQPAALRFLGAVLLLQVFALGEAALLPTYLLTAHQLRPAEVGLLSAVSIALSV
                     LGGVGAGVLYNRGAGIERVTAVALLVTLAGVLVCFHPAAPTAAVIAGRLVSAISGGAV
                     FAWISTRIPRIAPARDQIGLASGAVSQLLYIGMTAGPPALFALYELPGRAGFYGFILL
                     AYSLPLLAVARGAWRPVAEAAPAEPLHTIPPFQEF"
     gene            complement(15020..17353)
                     /locus_tag="VFF98_09980"
     CDS             complement(15020..17353)
                     /locus_tag="VFF98_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64000.1"
                     /translation="MAPISLRLGLSAARPALALALLSCAAATPALAHAAGNDTAAADA
                     DTDAGADARAILVTGAHRAAVGDAQEETAPAAIATLNAAQLGKLGGLDGAVTGALRLL
                     PGAHIGGGDFSGVSESTLSIRGFSQDQIGVARDGVPLNDPVFLTPHADFAGDPENYDS
                     ISVLYGSGSVDVPSFTTSGGSIDIRTVAPEKQAGILFKQGFGSNSLRRTFGRVNTGEY
                     HGFSAWVSGSVTSGHLWTDGGGEIWSRRFEANLQYKWGEENQINVIASDFKMKTNSYF
                     HPTLAQYAAEPYGQGYAQAAYPTGGGTNGVADVGTASANSLNLQRADFPIQTYGLNSR
                     FKLAEGLRLRIDPYYVRVNNGVAAVSATALSEGLIGTDLNGDGDKLDTRPVALGVFPV
                     QHRGGVTARLNWDAAKTNHVELGFWYDHVNSRYQLIAEPIAANGSPVSQTGSAQIYDS
                     QGRPVDFANQHNVIDTHKLWLQDAWDFLPGWNAVATLAWQHSSLDALNGAGIFTGSAY
                     ENKASYDRVLPGFALTFQANAANQFYYNLTTNERIPAIAGLYQGGASFTPQKAETTVN
                     QELGWRYATPQLLVSAALFLDYFHNRQVSYQAYSGVTSYFNAGNVTTRGAEVSLNGKL
                     PEHFSYSASWTHVIAKQDADYTLGGATAATAGRQLYNTPKDTLGWGLAYDDSRLYGNI
                     SGRYSAGVYGDLANTEKVPGYTTFDLALGYRFDHPAPFFRKVVLSLNLNNLFNKHALD
                     AVYGGSVSANAASSFYAAPTYSRLAPRTVLGNITLQL"
     gene            17756..20119
                     /locus_tag="VFF98_09985"
     CDS             17756..20119
                     /locus_tag="VFF98_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64001.1"
                     /translation="MTRPSTRLASPYIAGTSLASVSSLALLLAMAAPAQAATAAASTN
                     DAANASADADAADTANNIVVTAQHRKERLGDVPSAVTALDKDLFTTGGVGRSANEILN
                     YVPNASAGTTQHSRPRWWIRGVGAGQQQLDLASPVGFYLDDVYISNASATAIPLFDID
                     QVEILRGPQGTLWGKNTTGGAINVVSARPKLSGDQNNYVKLAYGSFDEKIIEGGLGAV
                     IVPDRLAVRASFHIDDADGRYTNVYTGQKSDAISDDVFRLQVLAKPAENLETLLGFHY
                     RRYDTQGAYWTVVSYAANGIYRNGYVPPSSTNQVSSNSPEWTNTRQLGGSLRNELHLG
                     GYTLTSISGYERFDSYGPADGDYTPLNISNTYSKAYSSQWTEELRIASPQNRKLTWLG
                     GLFYFNEKIHSDSYSATLPSGSVAALSGYSAVANYSVADYAHKAESGAVFGSATYAFT
                     DALKLTLGGRWTRETKTLEYNRLAAATTTTTTTASWSNYSDWWDNYTGTYGGAGTFGG
                     NLRRTWDAFTWDVTPNWHIDRNNLVYVKVAYGVKSGGFNTAATLPVALIAVNPERLHS
                     YEAGYKSQWLGGRLSFNATAFHYTYHDVQVNVVGPNPGAVGGATVSYLQNASRAHANG
                     AEFELAASPIENLKINAALGLLYTRFDSFQVLNGGANLSGNQFVRSPHTTLNVGAVYT
                     VPLAHAGKIDLEADARYTSLQYYFVTPQDAVNRPALAQRPYTLANARITYTAPGDHLS
                     ASFYVNNVLNTIYVNHALPAYNGSTVFGDVVQWADPRTYGVSLIYKF"
     gene            complement(20267..21424)
                     /locus_tag="VFF98_09990"
     CDS             complement(20267..21424)
                     /locus_tag="VFF98_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU64002.1"
                     /translation="MSIDFFWRLPTHGCHSSIRRGNYDRGDWSATGPHHLAPGLDGAG
                     EADGFAYIDHLAQIAKAAESVGFQGGLIPSFPNTDDPWVIAPLLARETRSFRFMIAYQ
                     PGFGNPVHFARASASLQRASGGRALFNIITGGGGPAQLWWGDSFAHDDRYGRTSEWLD
                     VFRGVWEHDSFDYDGRFYRVEGAHLPAHLRAEALPELWFSGSSEAALQVAARHADYYL
                     SWLEPFDQLADKFARVKERTAQLGGEQKVSVRVDLVARKTEEEAWADIARGFENVSEA
                     TRAQWRRRDGDSVGAARLAALRPTEATHWQDLKIAPGLWGGFNLLRGGPSIGIVGSYE
                     QCAEKLDELIRLGADGFILAGTPHLEEAWRVGEEVLPLLGQSAGALREAAE"
     gene            complement(21436..22392)
                     /locus_tag="VFF98_09995"
     CDS             complement(21436..22392)
                     /locus_tag="VFF98_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU64003.1"
                     /translation="MTLTIHWQLDVAAEPARGEPALRPALPPLLRDPRQTSQNRFDYY
                     AQVAEAAAQGRFDGLYLPFAADGDDSRIVAAALAREVRRIALVAGFPASVGSAVYAAK
                     QAVSFQRATGNRLGWAIAPDADAATRAGFGDFVPEAQLGQRLEEFLVVARGVHGQHPF
                     SHAGAFFAVEQGGFEAPLNRAAFPPVWLQGESEEALALSARQADVHLFAPTADLAALI
                     EGLDGLAKAAGRQVGFGLHQPVLARESTEEALFEAGRSPPPQGALVGSYEEVAGQLAR
                     RVRLGIGQLVLSAGPQLEEAYRIGQHLLPRLRALIATPAKAA"
     gene            complement(22389..23036)
                     /locus_tag="VFF98_10000"
     CDS             complement(22389..23036)
                     /locus_tag="VFF98_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU64004.1"
                     /translation="MTASAAATAPADAARRGADHPIHPLFTQRWSPRAYTGETVSDGV
                     LQSVFEAARWAPSARNLQPWRFIVARPGEAAWEDFLAILFERNRQWARRASALVAVVS
                     ETLAPRDGVPAPSESHAFDTGAAWANFAHQALLLGWHTRAMGGIDRAAARTTLALPED
                     FAVHVVVALGRQGALDDLPPEFHAQENPSSRRPLGDTVFAGRFGVPAFVPEKDAR"
     gene            complement(23036..23908)
                     /locus_tag="VFF98_10005"
     CDS             complement(23036..23908)
                     /locus_tag="VFF98_10005"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU64005.1"
                     /translation="MVDPFLLSSSAAPAPAPLARGALTITDLDKSFAIGGRPRQILSN
                     INLAVRPGEFVSIVGASGCGKSTLLRLIVGLDADYAGEIRLDGERVATTGLERGIVFQ
                     DHRLFPWMTLEQNIELALLNTGTPKERRRQIVAEHIALVNLGGFEKAYPHQLSGGMAQ
                     RAAIARALVTEPRLLLLDEPLGALDALTRVHVQNELQRIWLAQRSTMVMVTHDVEEAL
                     YLGDRVIVMAPNPGRIRRIVEVDLPHPRDRRSPLLHRLKDEILAELTAAPEPAARADG
                     APANLLPFAPREDR"
     gene            complement(23926..24708)
                     /locus_tag="VFF98_10010"
     CDS             complement(23926..24708)
                     /locus_tag="VFF98_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZU64006.1"
                     /translation="MSAPLIDRWPVRLAVGLVAPALLALWWQWQASLGGAHAAAFASL
                     ASLGGALGELIAQGTLLADTAATLLRALAGLALGASLGLGLGVALGVLPLLDRLLSPL
                     LNALRQVPMVGWLPLVGLWLGTGDAAALVVIGLSAFFPAMLNAHAGIAQVEPRYLEVA
                     RVLGFSPVQRLRLVLLPAALPLLLTGLTQAAAFTWIAAIGTEILQGTGGGLGVTMQLA
                     ETQQRLDIILVAIALTAFLGFALNQAVVLLRRHLLRWQAAPF"
     gene            complement(24705..25472)
                     /locus_tag="VFF98_10015"
     CDS             complement(24705..25472)
                     /locus_tag="VFF98_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64007.1"
                     /translation="MSRALLPLRRAAARLVPVLALLGLWQLVAGLGLVPPQVLAPPAR
                     VAATLAGMAASGELARHLGDSLHRLLLGFALGAVAGLAFGAATALSRWVQAALWPLFL
                     ALWQVPVIAFIPVLVLFLGIDEAFKITVVAVSAFFPVALATFDGIRALPRAWFDVARI
                     YRTRLGDLVWRILVPATVPAVLTGLRIGLTRAWIVLVAAELLAADSGIGQMMEMGRQL
                     FQIDVVLAGVVVSGLVGFLLDQGARQIEARATRWRHA"
     gene            complement(25469..>26177)
                     /locus_tag="VFF98_10020"
     CDS             complement(25469..>26177)
                     /locus_tag="VFF98_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64008.1"
                     /translation="GQGQDVYLVVRKDLPARSIADLKGKRIALHRGRPWELPFSKLLD
                     SAGLTFADFTIVNINPQATPAALAGGSVDATVLLSDGLLLEQKGIGRVIWSTKQAPAD
                     WKMRAELFARGDFVADHPEITQLVVSAFVRAARWSADEANKAQVIANAARGSMPTPII
                     EADYASTPYSWRERFSPLFRPAIASHYRSVADYALAHGLIHAPVDTGQLIEPRFVAQA
                     IRDLGLTGFWSDKDSAA"
BASE COUNT         4450 a         9339 c         8640 g         3748 t
ORIGIN      
        1 cggacggtgg tggcgctggc cctgcgcgat cccttgtgga gcgtgccgcg cccccgccgc
       61 tggttccgcc ccggcccgcc ccgggccgaa cggctgcaac tggccaaccc ccgcctcgag
      121 agcctgcgcc gtttcgtcat cctgcggcgc cacggtcagg atagcggcga tggcgccacc
      181 actttgctga actgcggctt cagccccggc cagatccgcg aggccggcag gctggcggat
      241 cttcactccg gcgcctgacc ggcccccttc ccctccccct cagcgcaggc ctggccaatc
      301 cacgcttccg gtggaggccc ccgcctgcct gatgccctga aaagagagca cgcatgaaga
      361 tttataccat caccgcctgt gccccgctgg ccctgttgac cgcttgcccc gcgccagcgc
      421 aggaaatcga tgccgcagcg caaaccgatg cggcccacaa cgacgatcat gtcatcctcg
      481 gcatgggggc gctctatgcc ccggcccatg aaggcggcga cacctatcgc gccctgccga
      541 tgcccgccat cgacatcaag gaggggcgct tctttgccaa tctgcgcgat ggcgtgggcg
      601 ccaacctcat cgacaccgat ctcctcaccc tgggcgccag cctcacctac acgcagggct
      661 atcgggcaaa ggatgtgccg gccggtgtcg gcgcggtcaa gctgggcgcg ggcgcgcgcg
      721 ggttcctctc gctccacgat caggggctga tcctcacgct gggcatgacg cagggctttg
      781 ccggcggcac caaggggctg atcgccgatg ccagcctgtc ctacccgatt gccgccacgc
      841 gccggctgac cctcatcccg accatcggca caagctgggc caatgccaaa ttcaacaacc
      901 gctatttcgg catcgatgcc gcgcaggcaa gcgcctcggg cctcaacacc tacgcccccg
      961 acgccggctt caaggatgcc agcggcatcc tcaccgccag ctatcagctc accgaccata
     1021 tcctcctgag cgcatcgggc gtggtgacca gggtgctggg cgcggatgcg aacagcccgc
     1081 tcgtggccca caagacgcag ccttccggct ttctgtcggt ggcctatcgc ttctgatcgc
     1141 ccgccttatg ccccggccac caggcgcgcc cagccatgca accgctgcca cagaatttgg
     1201 cgggtcgagg cgaagttgtg catatgcgcc atctcgggca cgataaacag cgatacatcg
     1261 ggtgacccag cgtaagccgc cggctcgcga tagggttcgg ggcaggtgtc ccgctcgccg
     1321 atgccgatca gcaccggcac ggtcacgctg gccgcatccg ccgtgaaaca gccgggcagc
     1381 atcatctgca cggcgcaatg cggaatggtg aggctgccga agcggggcga tgtctgccgg
     1441 atcggatagc cgccctgcat atcctcatgc aggatatcgg gcggcacatc ctcccagtgg
     1501 aaggcgtaga gataatccac gccctggtga tcatgctcga tgtggctggt gaccttgcct
     1561 tgcgccaccg catcgaagcg gcgcacccct tgcgcgaagg ccccggcgtc gcgctggggc
     1621 aaggccgtgt gaatggcgct gacgccgaaa ggggcaatgg catcgaaagt ggcaaagcgg
     1681 ccctgcgcga gaagggtgac cccgccgccc atcgactggc ccatgcccac cttgaacagc
     1741 gaaggcagcg ccggatagcc atcggccgcg gttccatcga ggaagcttgc cgttatcgcg
     1801 cgcacgcagg catcatgcgt tgccgcaagc gtctggaaac tgatcctggc aagatcgggg
     1861 atcgtgctct cgccaacgcc gacatgatcg atagcgacat ggatcacgcc gcgcccggcc
     1921 tgccaggcgc cttcatcgta atccgcatgg ccatcaaagc gcatcaggta atagtggcgc
     1981 gaatagccgc cgcccgggca ggcaaacacc gccaccgggc gttcgggcag gctggccgga
     2041 tcaggcaggc tgagcgtcac ggccatctcg atcggctcgc ccagctctgg cgctgcggcg
     2101 ctcacgtcga tccgcttttc tattctgagc acatcatcgc tcctgtcagc cgcgtgatat
     2161 tcgcatccgc cccgatcggg tacgcgcggc atcaggagag gcaaaatcaa atcgggggga
     2221 tccgatcatc tcagcgcgta tgtgatcaag gggaggcagc gccggtggcg ggcgacgggg
     2281 cagaagcggg gcccctttgc gaggggccat gcccatggct gcctattaac cacaatgtaa
     2341 acataattaa acattgattt tatattataa aatttctagc aagcgtcacc cgttgcgcgg
     2401 gtgcggggcg gccggttggc cagcttacgt cgtggcggag tttgctctcg tcggtggccg
     2461 ggtttgtcgc cccggtcacc cccgctctta ccgcgccacc cccgctctca ccgctcagcc
     2521 ttggcgccat cagcgccctg ccacccgccg cccagcgcct gatagagcgc ggccgtatcg
     2581 gcaagccgct gggcagccgc ggccgcttcg gcctgatcgg cctggcgcag ggcctgttcg
     2641 gcggccagcc gggtcatctc ggggatctcg ccaaggcggg cctggcggca tgccacctca
     2701 tattgcccct gcgccgcctg gcgggcctgt tccgccgcat ccagcgcggt ggcatcctcc
     2761 tgcagggcgt gcaacgtgtc gcccacattc tggaaggcgg ccagcacggc agagcggtaa
     2821 agctcgcgcg cctgatcgag cgtggccttg gccgcccgct gcttgtggag cagcgcgccg
     2881 ccggcgaaca gcggctggct cacccctgcc cccagcgacc agaagctgtt tgcccccgac
     2941 agcaggctgg cccagccggg cgaggagccg ccgccgctgg cgctcagcgt gatctgcggc
     3001 aggcgcgcgg caatggcgat gccaacgccg gcgctggcgg cgtggaggtt ttcctcggcc
     3061 gcgcgcagat ccgggcgctg gcgcaccagt tcggccggca ggctgagcgg cagatcgcgc
     3121 ggcaggcgca cttcggccag atcgacagac gccggcgggc cctctgccgg cgcgcggccg
     3181 gttagatagg ccagggcatc gcgcgcctgg gtccattgct tgcgcaaggg cggcagggcg
     3241 gcgcgcgcct gggccagcgc gccttcctgc gccatcagcg cggccccggc gatctcgccc
     3301 tgatcctgct ggcggcgggc gatatcgcgc tgcgcaaggg ccagttcgat gatccgctcc
     3361 tgggcggcca cctgcagatg cagcgaggct tccgtcagcg cggtggcgat caccgtgttg
     3421 atcagcgtga ggcgcgccgc ttcggcctca aagcgctggg cctgatactg ggcgcgggcc
     3481 tgctccacct gccggcggtt ggcgccgaac aggtccagcg tgtagcccac cgtcacctgc
     3541 gcggtctgga ggttatagtc gaagctgttg tcggccagcg ccggcgccag atattgcgtg
     3601 cccttggccc ggctcgtgct ggcccccgca tcgacgctgg gcagcagcac ccccttgctg
     3661 gccagccagc tttcccgcgc ggccttcagc gcggcgcggg tggcagccag atcggaattg
     3721 cgggccagcg cctctgccac cagcagatcc agcgcgggcg aatggaacgg gcgccaccat
     3781 tcggcgggca gcggggcggt gggcgaaagg gcctgcccgc cgccagccag cggcgctgcg
     3841 gcagcggcaa tcggctgcgg atcataggcc ttgccggcag gggccggcgg cgcccggaaa
     3901 tccggcccca ccgcgcagcc ggccagcacg gcggaggcgg cagtcagaag ggcgcagccc
     3961 gcggcgcgca gggatctggc ggtgttcatg cgctgggccc atccttggcg ggcgctgcgc
     4021 ccggcatcgc cacatcgtcc tcgtcgatat ccaccttttg cggctggcgg cgcgagaaca
     4081 tctcgatcat caccggcagc accaccagga tcagcaccgg cgccagcagg atgccgccca
     4141 ccaccaccag cgccagcggc ttttgcacct ggctgccgat gccggtggaa acggcggccg
     4201 gcagcaggcc cacgcaagcg gcgatgcagg tcatcatcac cgggcggaag cgggtttcgg
     4261 cggcatggag gatcgcctcc gagcggccca tgcccacccc cagcatctgg ttgtaatagg
     4321 ccagcaccag aatgccctcc atcaccgaaa tgccgaacag gccgataaag ccgatcgccg
     4381 ccgagacgga aaaagccgtg ccggtgaaga acagcgcata aatgccgccc accagcgcca
     4441 tcggcatcac gctggccgcc agcagcgtgt ctgtcagcga gccgaagttg atgaacagca
     4501 gcagggcaat cagcagcagg ctgatcggca cgacgatttc caggcgcgac agcgcatcct
     4561 gcagctcgcc aaattcgccc acccattcga ggcgggcgcc ggccggcagc gccacctgcc
     4621 ggtcgatcct ggcctgcgct tccagcaccg cgccgcccag atcgcgcccg cgcacggaaa
     4681 acttgatggg gatgtagcgg gtctggttct cgcggtagat gaaggaatag cccgacacca
     4741 gctgcacatc ggcaatggtg gacagcggga tctggctggt gcccccgctg gcattggcca
     4801 ccgccacggg aatggcgcgg atggcatcca ggctgccgcg gaactgcggt gccatgcgga
     4861 tgatgatggg gaaattgcga tcagagccat attcgtaaag gttgccggcc gcttggcccc
     4921 caatggccgc ctgcaccacc gcattcacat ccgccggcgc caggccatag cgggccgccc
     4981 tgttgcgatc gattttgatg ttgatggttg gctggcccag ggcatcgaac accgacagat
     5041 cggtgatgcc cggaacgccg gccatgaccg ccttgatctg ataggcggtc ttttccagcg
     5101 cctccagatc attgccatag agcttgaccg agttctgccc cttcacgccg ctggcggctt
     5161 cctcgacatt gtcctcgatg ttctgcgaga agttgaactc cacccccggg aaaggcgcgg
     5221 ccagatcggc gttgacctcc cgcgtcagct tttccttggt catcccccag ggccatgtgt
     5281 tgccgggctt cagcggcaca tagaattcgg cgttgaaaaa gcccgtggtg tcggttccgt
     5341 catcgggccg gcccagctgg ctgacaacgg ttttcacttc gggatatttc atcaggatat
     5401 ggcgcatgcg gttcacatag ccttgcgcct cctccaggct caccgtgggg ggcagcgtgg
     5461 cgcggatcca gaagttgccc tcttccagct tgggcagaaa ttccagcccg aggctgctgc
     5521 tggccagcac cgcccccagc agcagcgcgc ccatgccgcc cagcgccacc acccggttgg
     5581 ccagggcaaa gcggatggcg ggcgtgtaga gccggcgcat ccagcgcacg atcggcgttt
     5641 cggtttcctc ttcctgctcc ttcagcagca ccgccgaaag caccggcgcg acggtgaagg
     5701 tggccagcag gccgccgatg atggcatagg cataggtgcg cgccatcggc ccgaagatgt
     5761 gcccttccac acctgtcagc gtgaacagcg gcaggaagcc ggcgatgatg atggccgcgg
     5821 caaagaagat cgcccggttc acctcggcgg ccgacagttc gattgtgccc agcttgccga
     5881 agaagctctg gatgccgccc ttgcgatcca gcgtgccacc cttgtggaag cggtcgcgcc
     5941 ggggctgggc aaggtggcgg aagatgttct ccaccatgat caccgtggca tccacgatca
     6001 gcccgaaatc gatcgcgccg acagacagca gattggccga ttccccgtgc agcaccataa
     6061 tgatgatcgc aaaggccagc gcaaagggga tcgttgccgc cacgatgagg gcgctgcgca
     6121 gcgtgcccag gaagatccac tgcaccagga agatcagcac cacgccgaag atcatgttct
     6181 ccagcacggt gtgggtcgtc acgtcgatca ggtcggtgcg gtcgtagatc ttttccaggt
     6241 gaacgccggg cggcagcacg ccctcggcat tgatcttggc cacctcggcc tcgacgcgct
     6301 gcagcgtcgg caggctttcc tcgccccggc gcatcaggat gatgccctgc acgatatcgt
     6361 cgtcattatc ctggccaacg atgcccaggc gcggctggtt gcccacttcc acgctggcca
     6421 catccttgac ctgcaccggc ttgccgctca ccgaggcaat catcgtgtcg cggatatcgt
     6481 ccatgctgtg gatcaggcca acgccgcgca ccaccgccga ttgcggcccc aaattcaccg
     6541 tctgcccgcc gacattggcg ttggcgttgt tgagcgcgct cagcacctgg ccgatcgaca
     6601 ggccataggc caccagccgg cgctggtcga tcgtcacctc ataggtcttg ctcttgcccg
     6661 cccagccgtt gacatcgacg acgcccggca ccgccttgaa gcggcgctgg aggatccagt
     6721 cctgaatggt cttcaggtcg gtgaccgaat agcccttcgg cccgacgatg cggtagcgat
     6781 agatctcgcc gatcgggctt tccggcgaaa tgcccgcctg cgcgcccccc ggcagcggcg
     6841 gcagctgcga cagcgcgttg atgaccttct gctcggcctg ctcatagcca taatcgtagg
     6901 tgaactgcac gcgcacgtcc gacaggccga acagcgagat cgtctccacc ttcttgacgt
     6961 tcttgatgcc cgccagctgc acctcgacgg ggacggtgat ggtgcgctcg atctcgccgg
     7021 ccgacaggcc cgggctttgc gtcaccactt ccaccagcgg cggcaccgga tcgggatagg
     7081 cctcgatgtt gagctggcgg aaggcgaaca gcccgcccag caggaacagc cccagcagcg
     7141 ccaggatcat caggcgctgc ttgagcgcga aggcgacaat cgggttcatc tgcgctcaat
     7201 ccgctttggc gccgcgatcg atgaacaggc tgcccgccgt caccaccgtc tcgccggcct
     7261 gcagcccggc ggtgatctcc acccggccgt cgaaagtggc gccagtgccg atggcgcgca
     7321 gggctaggcg atggccggat ggctgcgcca cccacacccg cgcggttgcc ccctcgtaga
     7381 tcaccgcggc ttccggcgct gtgagcacgg tgcgcgcgcc gcctgtcagc aggcggaagc
     7441 tggcgaacat ctcgggcttc agctggccgc cggcgttcgc caaagaggcc cgcaccgtaa
     7501 gccgccgcga ggccggatcg agcgcggagg ccacataatc cacccgcgcg gccagcggat
     7561 gatcgggatc caccagcagg cgcacttcgg ccagctggcc cacatgcgcc ttggccgcat
     7621 cctcttcacg caggttgccc accacccaca cctggccaaa atcgctgatg gtgaaagcct
     7681 gggtggccgc cccgccagaa gcgctggcca cattctgccc caggccgatc agccgctggg
     7741 tgacgatgcc cgccaccggc gcccgcacca ccccctcgcc ccgcgacagg ctgccgccca
     7801 gcgccaggcg ccgcgcctcg gccgcattca gcgccgcgcg ggcactggcc aggttcacct
     7861 cggccgcttc cagatccttt tccgccgcgc cctggtggcg cgacagatcg cgcatgcgcg
     7921 ccgcctccgc ctcggccgcc ttcagcgtgg ccacgcccgt cagcatgtcg gcatcgttct
     7981 gcgcggcttc attggcgatc accgcgaaca gcggatcgcc cgcgcgcacc ctgtcgccca
     8041 cttttgcaaa cacccgcgtc acccgcccgg taaagggcga gagcacctgc gtggtgcgat
     8101 catcatcaac ggcgatcttg ccatcggtga caacgccgtt ctgccaggtc tggctgcgca
     8161 gggtttcaaa acgcagggtc tgccattgcc tgtcggtggc ggcaaagctg ccgtcggccg
     8221 gcgctggggc cggggcggca gggggcggcg ccatcaggct ccacagccgg ggcgctgcgc
     8281 caaacaccag caggctggcg accaccgccg ccccggccag gcggatctgc tgccggcgat
     8341 tgagttgcgg cccggaaggc gaatgggtcg gcgcaggaag atccgtcatc ggcaagccat
     8401 atcctgtgga tagcaatggc cgcgatctag agccgatggc ttacatcgtc cttacggcgc
     8461 cccctgcccg tgcacggccg catcgccaaa gcggatggcg aagcgggcgc cttttgccgc
     8521 tgccgggccc gcttccaggc gcgcgcgatg ggcatcggcg atccgctgga cgatcgacag
     8581 ccccagcccc gcgccatccg tgcgggcatg atcggccctg tggaagcggc gcagcaggcg
     8641 cggcccgtcc tcttccagaa tgccatggcc attgtcgatc accgccagca cgcctgcccc
     8701 ctccagcgca acggtgatat gggtgcccac cggcgtgtgc acggccgcat tctcgatcag
     8761 attggccagc gcgatggcca gcagcccggc attgcccagc acgctcatgc cctcgccgcg
     8821 cagctcgatc gtgcggcccg ccgccagaat gcgcgggctc agctccgcca ccacgcccac
     8881 cgccagatcg cccagatcga ccggcgccat cgaggctgcc gcggcccctt ccagcgccgc
     8941 cagatcgcgc aattgcgaca gcacatggcc catccgctcc acctgcagcg ccatggcctg
     9001 ccgcaggggc ggctcgacca cctgttcggc gcgcaggcgc agggcggcca gcggcgtgcg
     9061 cagttcatgg gccacattgc cggcaaactc gtcctgctgg tgcagcgcct ggtgcacccg
     9121 ggccagcaat ccgttgatcg cctgcaccag cggccggatc tcggagggca gcccctgttc
     9181 gcccagctgc aagctgaacg cgcgcgggcc gatctccgcc gcctgccggg tgatcgccct
     9241 gaggccgcga aaggtgtgcc acagcagcag gccattgatc gccagcatca gcatcagcag
     9301 gccgatcagc gccacgagat agcgcgccag aaaggcccgc atcacatcat ccaccaccgc
     9361 ccccggccgg ccctgatcct gcgtgaccac cagccagccc cgctggccgg ccaccgccac
     9421 cggcaggctg agcccggtga gcggcccggc ctcgaagcgc atggcgcgcg cggcatagat
     9481 gcggcccggc caggcgcggg gctgcgcata gcggctgatg ctgaccggat ggccggcgcc
     9541 atccatcagc gcataggcgc gcccgtcgta accggaatcg aacgtggcgc gcaccgtgtc
     9601 gcccggctgc agcgtccagc ccctgcgggc gtcataggca aggccgccgc gcagggtttg
     9661 cgcctgcccc atcagcatct ccgcctgaat gcgctcggcg ctgctgcgca gttccgacag
     9721 ggtcagcagc ggcagggcaa tgcaggcggc aaggaaggtc agcgcctcga tcagcaggat
     9781 gcgcagcagc atcgagcggg cgatgggccg aaacgggcgg atcatgccgt gccctgcagc
     9841 atcaggccgc gccctgggag agatcgctca gcagatagcc cacccccctg atcgtcacca
     9901 cttccacccg ggcgccggcc gcgctcagct tgtgccgcag gcggtgcaca taaacctcga
     9961 tggcattgga tcccaggcca tcgccgccaa acagctggtc ctcggccagg cgcctcgtca
    10021 gcacctggcc cgagcggcgc agcagcagcg ccagcaagct tgcctcgcgc gcggacaggt
    10081 tgaccagcac gccatccacc tgcaacgaca gcgcatccag atcgaaggcc atattggcaa
    10141 aatgggcgac cgggcccaga aacgtgccat tgcgcctgag gatggcgcgc agccttgcca
    10201 ccagctcatc gggcgagaag ggcttggcca gataatcatc ggcgccggca tccagcccgg
    10261 caatgcgcga tgcgatttcc gaccgcgcgg tgagcatgat caccggcaca ttatgcccct
    10321 gccgcctgag gccacgcagc aggtccagcc cgtcgccatc gggcaggccg cgatcgagga
    10381 tcatcgcggc atattggctg gtggccagca gcagggcggc atcctcgccc ttgccggcca
    10441 gatcgacccc atagccgttc tggcgcagat gatcgctcac cgccagcgcc agcgccctgt
    10501 catcttccac cagcaacagc cgcatgccca ccacccccct gatcgttccg caggcgcgat
    10561 ggctggcgcc tagcccatcg cgggccaaat ggcgagcggc ccgttatgcc agcggcccgt
    10621 tatgccagag gcaccgcatc caccacctgc agcgcaacat attcgtcata gccgatgcgg
    10681 ccgccgcgat agccgatccc gctccacttg tggccaccgc ctggcgctgc cggaaacggc
    10741 cgggcatgct ggttcaccca gccatagccg acatccagcc gctgcacgat ccgcgccgcc
    10801 tgcgccacat cggcgctcca caccgatccg ccaaggccga agggggtgtc gttggcggcg
    10861 gcaatcgctt cttccacatc gtcgaagggg atcaccggca acaccggccc gaactgctcc
    10921 tcatcgacca gcgccacccc cttgccgatg ccggtgacga tggtgggcgc atggaaatag
    10981 ccctcggccg gcagcggggc ctgaccctga tgcagcaccg cgccatgggc cagcgcatcg
    11041 gccaccagcg cgcgcacccg gtcgaactgc tcgcgggtgg tcagcgggcc cagcgtgctg
    11101 gccggatcga gcccgggccc gggccgcgcg gcatcggcca gcgccgccag cgccgccacc
    11161 agcggggcaa acaccgcccg cgccgcataa acgcgcttca ccgcaaagca gatctgcccg
    11221 gcgttgaaga aggccgcctc gaacagcgcg cgggccacct tctccaccgg cgcatcggcc
    11281 agcacgatgg ccgcgtcatt gccgcccagt tccagcgaca cgcgcttgag atcgccggcc
    11341 gtttgcgccg ccaccgcctt gccgccgcgc acgctgccgg tgaaggtgac cttggcgaca
    11401 tccgggtggc gcgtcagctc cgcgcccagt tccttgccgc cggcaaggat gttgagcacg
    11461 cccgccggga agatatccgc cagcgcccgg cccagcgcca ggctcgacag cggcgtgtgt
    11521 tccgacggct tggccaccac cgtgttgccg gccagcagcg ccggcacgat atcgcgcagc
    11581 agcagcagca ccggcacgtt ccacggcgtg atcgccgcca ccacgcccag cggcgcgcgc
    11641 accacctgca ccggcgccgc gcccggcctt tcagccagca cctcgtcggg cacggtgaag
    11701 gcggcatagc tgtcgaggat ggcggcgcca tattgcacct cgcccagcgc ctgggccagc
    11761 gggcggccct gctcgcgggt gatcagctgg gcgatggggc cagccctttc gcgcaggcgt
    11821 tcagccgccc gggccagcac ctgccggcgc agcggctcgt ctgccgccca ggccgggaag
    11881 gcagcgcgcg cggccgccac cgcccgttcc agctcggccg tgccggcctg cggggcatgg
    11941 gcgaaaaccg cgccgctggc cggatcgatc accggatcgc tggccgtggt ggcaacggcc
    12001 gcgccgtcga tcaacagggt atattcggtc atctcggatc tccgtcgaaa gaactcaagc
    12061 aggcagggcg gcgctggcgg gcgccagggc gtggcgggca aggaaggcat cggccagctt
    12121 ttcggcggcc atcagcgtgg gcaggttgat gttggcggaa ggcacgccag ggaacaccga
    12181 ggcatcgccc acccacaggc ccgccacgcc atacacccgc ccttccggat cgacgaccgc
    12241 cagcggatcc tccgcccggc ccatgcgcgc ggtgccgcag gcatgccaca cgccgctgac
    12301 gttgtagcgc aggtggcgca ccagcgcggc atcatcggcc agcagctctg ccagcggggc
    12361 gctgatgccg ccctcgggca cgccgaagcg atagagctgc ggggccaggc caaagggcgc
    12421 cagccgggca tcccccagca ccgcggccag ccgccgaagg ccgatccgca gccgatcgat
    12481 atcgcgcgga tcggccagca ggttgagatc gcgcagcggc ctgtcggcca ggcccgggcc
    12541 gctcagcgcg acacggccga cagaatccgg ccggttgatc cacagccccg ccatcatccc
    12601 ttccggcgag gcggtgatca ccgcgaacag atcggccgga tccgcccccg gcaggcccga
    12661 ggagaaacgc gcgcccaccc ccgaacggcg ccacccctgc ggccactgcc cgcccgcaac
    12721 cggcgagacg agggcgatcg aggcatgttc ccacagcccc cggcccacgc ccggccggtc
    12781 tgccacaggg gcaatgccca gcgcccgcag ctcgtcggcc gggccgatgc cggcgcgcag
    12841 cagcagcacc ggcgtatgca acgcgccggc ggccaggatc actcgccgcg ccgccagcgg
    12901 ccggggcgcg gccggcgtgc cgatatccac cccgcgcacc gccccgccct cgaccagcag
    12961 ggcgctgacg gtggtatccg taaggagggt gagattggcg cgcgcgcgca ccgtggcatc
    13021 gagatagcca aaggcggccg agctgcgctg ctgcccatca tgcgattgcg ccatgcggaa
    13081 atggccggtg cgaaaatcgc cgttctgatc ttccagaaag ggcaggccat gatcggccag
    13141 caccgccgac accgcccggt tgaagcccga ccagtgctcc agccggtcgt ggctgatggg
    13201 gatcgggccg ctgccgccat gcagggggcc ctcgccaaag gtagtgtcgg tttccagctt
    13261 gcggaaatag ggcagtaccg cctcccagtg ccagccttcg gcgccgcgcg cggcccagtc
    13321 gtcgtaatcg gagggcacgc cgcgattggc cgcctgcatg ttgatgctgg agccgccgcc
    13381 caggatgcgc gcctgctcgt aatagcggcc ggggggctgg tcggcatggc gggtggtgta
    13441 aaaatcgggc cacatgaagc gcgcgccgct gcgcaagggg ccgctggcat cgctcgcatc
    13501 gaggatctcg ggcggggcgg tgtcgggggt caggtcgggc ccggcctcga tcagcagcac
    13561 cgacagcgcc ggatcggccg agaggcgccc ggccagcacg ctgcccgccg agccgccgcc
    13621 cacgatgatc acgtcaaacg ggccggcggg aaaggaaaat gggggggcca tgtcagaact
    13681 cctgaaacgg ggggatggtg tggagcggtt cggccggcgc tgcctcggcc acagggcgcc
    13741 aggcgccgcg cgccacggcc agcagcggca ggctgtaggc cagcaggatg aagccataga
    13801 agccagcccg gccgggcagt tcgtaaagcg caaagagcgc cggcggccct gccgtcatgc
    13861 cgatgtagag cagctggctg accgcgcccg aggcaaggcc gatctgatcg cgggccggcg
    13921 cgatgcgcgg gatgcgcgtg ctgatccagg caaacaccgc cccgccgctg atcgccgaga
    13981 ccagccggcc ggcgatcacc gccgcggtgg gcgcggccgg atggaagcac accagcacgc
    14041 cggccagcgt gaccagcagg gccacggcgg tcacccgctc gatgccggcg ccgcggttgt
    14101 agagcacccc tgcccccacc ccgcccagca ccgaaagggc aatcgacacc gccgagagca
    14161 gccccacttc ggccgggcgc agctggtggg cggtaagcag ataggtgggc agcagcgccg
    14221 cctcgcccag cgcgaatacc tgcagcagca gcaccgcccc cagaaagcgc agcgccgccg
    14281 gctggcgata gaccgcccag ggcgaagccc cgccacgctc cgaagcgcgc gcgcgtgggg
    14341 gcgcggccgg cagcagcggc gaggccacca gcgccagcag cagcagcccg gcgtggccgg
    14401 caaacaccca gcgccactgc gccgtgcccg ccagcggcga caccgccaga atggccagcg
    14461 cgccgcccat cggcgaggcc accgaccaga atgccagcgc cgtggtctgg cgcgggccgg
    14521 aagtggtgcg gataagcagc gacaggcagc ccagcaccac cgcgatatag cccgccccct
    14581 cgatcacccg cagcagcacc agctcggcaa agcgcggcac gaaggcggcc gccaggctgc
    14641 tgggggcggc cagcgccagc cccaggcgga tggcccggtg ttcgcccagc cggtcgatca
    14701 gccagccgcc aaagggcgcg cccaccgccg ccaccagcgc caccgccgcg atcacccagc
    14761 tggcccgcgc cggatcaacg gcaaagctgc gcgccacatc gggcaccagc agcagcgtct
    14821 tgccgtggca ggcggcgatc agctcggcat aggcatagat cgccagaatg gccggccagc
    14881 tgccggcgcg ggtggtcggc aggcgagaga ggaagggctg cggcccatcg gccggcaggg
    14941 atgtatcggt ggtcatcggc agctccgcga aaggggggaa ggcccggggc gccgcgccag
    15001 tgcgctgcgc ccctgcggct cagagttgca gtgtgatgtt gcccagcacc gtgcgcgggg
    15061 ccagccggct gtaggtgggc gcggcataga aggagctggc cgcattggcc gaaaccgacc
    15121 cgccatacac cgcatcaagc gcatgcttgt tgaacaggtt gttgaggttg aggctcaaca
    15181 ccaccttgcg gaagaagggt gcggggtgat cgaagcgata gcccagcgcc agatcgaaag
    15241 tggtatagcc cggcaccttc tcggtattgg ccagatcgcc atagacgccg gcgctataac
    15301 gccccgaaat attgccgtag agccggctgt cgtcataggc caggccccag cccagcgtat
    15361 ccttgggcgt gttgtagagc tgccggccgg cggtggcggc cgtcgccccg cccagcgtgt
    15421 aatcggcatc ctgcttggcg atcacatgcg tccacgatgc cgaatagctg aagtgctcgg
    15481 gcagcttgcc gttcagcgaa acttccgcgc cccgcgtggt gacattgccg gcgttgaaat
    15541 aggaagtgac gcccgaatag gcctgatagg acacctgccg gttgtggaaa taatcgagga
    15601 acagcgctgc gctcaccagc agctgcggcg tggcatagcg ccagcccagc tcctggttca
    15661 ccgtggtttc cgccttctgc ggggtaaagc tggcgccgcc ctgatagagg ccggcaatcg
    15721 ccgggatgcg ctcgttggtc gtcagattgt agtagaactg gttggcggca ttggcctgga
    15781 aggtcagcgc aaagccgggc agcacgcggt cgtagctggc cttgttctca taggcgctgc
    15841 cggtgaagat gcccgccccg ttcagcgcgt cgagcgagct gtgctgccag gcaagggtgg
    15901 ccaccgcatt ccagcccggc aggaaatccc aagcgtcctg cagccacagc ttgtgggtgt
    15961 cgatcacatt gtgctggttg gcaaaatcaa ccgggcggcc ttgcgagtca tagatctgcg
    16021 cgctgccggt ctggctcacc gggctgccat tggcggcaat cggctcggcg atcagctggt
    16081 agcggctgtt cacatgatcg taccagaagc ccagttccac atggttggtt ttggccgcat
    16141 cccagttcag ccgcgcggtc accccgccgc ggtgctgcac gggaaacacc cccagcgcca
    16201 ccggccgcgt atccagcttg tcgccatcgc cgttgagatc ggtgccgatc agcccttccg
    16261 acagcgccgt ggccgacacc gccgccacgc cattgttcac ccgcacgtaa tagggatcga
    16321 tgcgcaggcg cagcccctcg gccagcttga agcggctgtt gaggccatag gtctggatgg
    16381 gaaaatcggc gcgctgcagg ttgaggctgt tggccgaagc ggtgcccaca tcggccacgc
    16441 cattggtgcc gccgccggtg ggataggccg cctgcgcata gccctggcca taaggctcgg
    16501 cggcatattg cgcgagggtg gggtggaaat aggaattggt cttcatcttg aagtcggagg
    16561 cgatgacgtt gatctggttt tcctcgcccc atttgtattg gaggttggcc tcgaagcggc
    16621 gcgaccagat ctcgccgccg ccatccgtcc acagatggcc cgatgtgaca gagcccgaca
    16681 cccaggccga aaaaccgtga tattcgccgg tgttcacccg gccgaaggtg cgccgcaggc
    16741 tgttgctgcc aaagccctgc ttgaagagga tgcccgcctg cttttccggc gccacggtgc
    16801 ggatgtcgat gctgccgccg ctggtggtga agctgggcac atcgaccgag cccgagccat
    16861 agagcaccga gatggaatca taattttccg gatcgccggc gaaatcggca tgcggcgtga
    16921 ggaaaaccgg atcgttcagc ggcacgccat cgcgcgccac gccgatctga tcctgcgaga
    16981 agccgcgaat gctgagcgtg ctttccgaca cgccggagaa atcgccgcca ccgatatgcg
    17041 cgccgggcag cagccgcagc gcgccggtca ccgcgccatc cagcccgccc agcttgccca
    17101 gctgcgccgc attgagcgtg gcgatcgccg ccggcgcggt ttcctcctgc gcatcgccca
    17161 ccgccgcccg gtgcgcgccg gtaacaagga tcgcccgggc atcggccccg gcatcggtat
    17221 cggcatcggc agcggcggtg tcgttgccgg ctgcatgggc cagcgccggc gttgctgcag
    17281 cgcagctcaa taaggccagc gccagcgcgg gccgcgcggc agacaggcca aggcgaagag
    17341 aaatgggggc catttttgca aatccctgtt gagaataccg aggatcgcac cgcccggaag
    17401 gaaggaaggg gcggccgaac agcggttcct ttcagcagga agcgtgccat cctctgattt
    17461 atcgcatttt tcgcagcccg cgcccggccg ccctggtgat atcgccgcaa catatgcgcg
    17521 gcttgtgctg catatgcagc atggccgccc tgccccgcgg caaaggcccc cgccccgccc
    17581 ggcgcagcgg ggatttcacc agcgccattg ctgcgtttgc accaccgccc cgcggcgcca
    17641 ccccggcaaa ggccggcaat ccgccattgc gcgcaagtgg cacgcgcttt gctgaacagg
    17701 gggcaacatc caaccgcgcg gcttgcgcgc acccagacac accgggggga atatcttgac
    17761 cagaccgtcc acgcgccttg ccagcccgta tatcgccggc accagccttg cctctgtcag
    17821 ttcactggcg ctgctgctgg caatggccgc gcccgcgcag gccgccaccg ccgccgccag
    17881 caccaacgat gccgccaatg ccagtgccga cgccgatgcc gccgacaccg ccaacaacat
    17941 cgtcgtcacc gcccagcacc gcaaggaacg gctgggcgat gtgcccagcg ccgtcaccgc
    18001 gctcgacaag gacctgttca ccactggcgg ggtcggccgc tcggccaacg agatcctgaa
    18061 ctatgtgccc aatgcctcgg ccggcaccac ccagcacagc cgcccgcgct ggtggatccg
    18121 cggcgtgggc gctggccagc agcagctcga tctggccagc ccagtgggct tctacctgga
    18181 cgatgtctac atctccaacg ccagcgccac ggccattccg ctgttcgata tcgatcaggt
    18241 ggagatcctg cgcgggccgc agggcacgct gtggggcaag aacaccaccg gcggggcgat
    18301 caacgtggtc tcggcccggc cgaagctgag cggcgaccag aacaattatg ttaaactggc
    18361 ctatggcagt ttcgacgaga agatcatcga gggcggcctt ggcgcggtga tcgtgcccga
    18421 ccggctggcc gtgcgcgcct cgttccacat cgacgatgcc gacgggcgct acaccaatgt
    18481 ctacaccggg cagaagtcgg atgcgatttc cgatgacgtg ttccgcctgc aggtgctggc
    18541 aaagccggcg gaaaacctcg agacgctgct gggcttccac tatcgccgct acgacaccca
    18601 gggcgcctac tggacggtgg tgagctatgc cgccaacggc atctatcgca acggctatgt
    18661 gccgccctcc agcaccaatc aggtgagcag caattcgccg gaatggacca acacccgcca
    18721 gctgggcgga tccttgcgca acgagctgca tctgggcggc tacacgctga cctcgatcag
    18781 cggctacgag cgtttcgaca gctatggccc ggccgatggc gactatacgc cgctcaacat
    18841 ctcgaacacc tattccaagg cctattccag ccagtggacc gaggagctgc gcattgcctc
    18901 gccgcaaaac cgcaagctga cctggctggg cggcctgttc tatttcaacg agaaaatcca
    18961 ttccgacagc tattcggcca ccctgcccag cggcagcgtg gcagcgctgt cgggctatag
    19021 cgcagtggcc aattacagcg tggccgatta tgcgcacaag gccgaaagcg gcgcggtctt
    19081 cggcagcgcc acctatgcct tcaccgatgc cctcaagctg acgctgggcg gccgctggac
    19141 gcgcgagacc aagacgctgg aatacaaccg cctggccgcc gccaccacca ccacaaccac
    19201 caccgccagc tggagcaatt acagcgactg gtgggacaat tacaccggca cctatggcgg
    19261 ggcgggcacg tttggcggca acctgcgccg cacctgggat gccttcacct gggatgtaac
    19321 gcccaactgg catatcgacc gcaacaatct ggtctatgtg aaggtggcct atggcgtgaa
    19381 gtcgggcggg ttcaacaccg ccgccaccct gcccgtggcg ctgatcgcgg tgaacccgga
    19441 acggctgcat tcctacgagg cgggctacaa gtcgcagtgg ctgggcgggc ggctcagctt
    19501 caatgccacc gccttccact acacctatca cgacgtgcag gtgaatgtgg tgggccccaa
    19561 tccgggcgcg gtgggcggcg ccaccgtttc ctatctgcag aacgcctcgc gcgcgcatgc
    19621 caatggcgcc gaattcgaac tggccgccag cccgatcgag aacctgaaga tcaatgccgc
    19681 gctaggcctg ctctacaccc gcttcgacag cttccaggtg ctgaacggcg gcgccaatct
    19741 ctcgggcaac cagttcgtgc gctcgcccca caccacgctg aatgtgggcg cggtttatac
    19801 cgtgccgctg gcccatgccg gcaagatcga tctggaggcc gatgcgcgct atacttcgct
    19861 gcaatattat ttcgtcacgc cgcaagatgc cgtcaaccgc ccggcgctgg cccagcggcc
    19921 ctacacgctg gccaatgccc gcatcaccta caccgcgccc ggcgatcatc tcagcgcctc
    19981 gttctatgtg aacaatgtgc tgaacacgat ctacgtcaac catgcgctgc cggcctataa
    20041 tggcagcacg gtgtttggcg atgtggtgca atgggccgat ccgcgcacct atggcgtatc
    20101 gctgatctac aagttctgat ctccccggcc ggaagggggg atggggccgc ataccggggg
    20161 gatgcggccc ctttttcatg ggtgctggca ggggctggcc ggcgggcacg cgaaaaggcg
    20221 ccggaagaac ccttccggcg ccttcgctcc tctcctctgc aggaacctat tcggccgcct
    20281 cgcgcagggc cccggcgctc tgccccagca gcggcagcac ttcctcgccc actcgccagg
    20341 cctcttccag atgcggcgtg ccggccagga tgaagccatc ggcgcccagg cggatcagct
    20401 cgtccagctt ttcggcgcat tgctcgtagg agccgacgat gccgatggat ggcccgccgc
    20461 gcagcaggtt gaagccgccc cacaggcccg gggcgatctt cagatcctgc cagtgggtgg
    20521 cctcggtggg gcggagcgcg gccagccggg ctgcccccac cgaatcgcca tcgcgccgcc
    20581 gccactgggc gcgggtagcc tccgagacat tctcgaagcc ccgggcaata tcggcccagg
    20641 cctcctcctc ggtcttgcgc gccaccagat caacccgcac cgacactttc tgctcgcccc
    20701 ccagctgggc ggtgcgttcc ttcacccggg cgaatttgtc ggccagctgg tcgaacggct
    20761 ccagccacga gaggtaatag tcggcatggc gcgcggccac ctgcagcgcg gcttccgacg
    20821 agccggaaaa ccacagttcg ggcagcgcct cggcgcgcag atgggccggc agatgggcgc
    20881 cctccacgcg gtagaagcgg ccgtcatagt cgaagctgtc gtgctcccac acgccgcgga
    20941 acacatccag ccattcgctg gtgcggccat agcggtcgtc atgcgcgaag ctgtcgcccc
    21001 accacagctg ggccgggccg ccgccgccgg tgatgatgtt gaacagcgcc cgcccgccgc
    21061 tggcccgctg caggctggcg ctggcccggg cgaaatgcac cggattgccg aagccgggct
    21121 ggtaggcgat catgaagcgg aaagatctgg tctcgcgcgc cagcagcggg gcgatcaccc
    21181 aggggtcgtc ggtgttcggg aaagagggga tgaggccgcc ctggaagccc acgctttcgg
    21241 ccgccttggc gatctgggcg aggtggtcga tataggcaaa gccatccgcc tcgccggcgc
    21301 catcaagccc cggggccaga tggtgcggcc cggtggcaga ccagtccccc cggtcgtaat
    21361 tgccgcggcg gatgctggaa tggcagccat gggtgggcag gcgccagaag aagtcgatgc
    21421 tcatggatgt gctcctcagg cggccttggc gggggtggcg atcagcgcgc gcaggcgcgg
    21481 caacaggtgc tggccgatgc gataggcctc ttccagctgc ggcccggccg acagcaccag
    21541 ctggccaatg cccagccgca cccgccgcgc cagctggcca gccacctcct cgtaggagcc
    21601 gaccagcgca ccctgcggcg gcgggctgcg ccccgcctcg aacagcgcct cctcggtgct
    21661 ttcgcgcgcc agcaccggct ggtgcaggcc aaagcccacc tgccgcccgg cggcctttgc
    21721 cagcccgtcc agcccctcga tcagggccgc aagatcggcg gtgggggcaa acaggtgcac
    21781 atcggcctgc cgggccgaca gcgccagcgc ctcctcgctc tcgccctgca gccacaccgg
    21841 cgggaaggcc gcccggttca gcggcgcctc gaaaccgccc tgctccaccg cgaagaaggc
    21901 gccggcatgg gaaaacggat gctgcccatg cacgccgcgc gccacgacaa ggaattcctc
    21961 cagccgctgg cccagctgcg cctcgggcac aaaatcgcca aagccggcgc gggtggcggc
    22021 atcggcatct ggcgcgatcg cccagcccag ccggttgccg gtggcccgct ggaagctgac
    22081 cgcctgcttg gccgcataga ccgccgagcc gaccgaggcg ggaaagccgg ccaccagcgc
    22141 gatgcgccgc acttcgcggg ccagcgcggc ggccacgatg cggctgtcgt cgccatcggc
    22201 ggcaaagggc aggtatagcc cgtcgaagcg gccttgcgcg gcggcctcgg ccacctgcgc
    22261 gtaatagtcg aagcggttct ggctggtctg gcgcggatcg cgcagcaagg ggggcagcgc
    22321 cggccgcagg gccggttcgc cgcgggcagg ctctgcggca acatcgagtt gccagtggat
    22381 cgtcagcgtc atcgcgcatc cttttcggga acgaaagccg gcacgccaaa gcggccggca
    22441 aacacggtgt cgcccagcgg gcgacggctg ctggggtttt cctgcgcgtg gaattcgggg
    22501 ggaaggtcat ccagcgcgcc ttgccggccc agcgccacaa cgacgtggac ggcaaaatcc
    22561 tcggggagag caagggtggt gcgggccgcg gcccggtcga tgccgcccat cgcccgcgtg
    22621 tgccagccca gcagcagcgc ctgatgggcg aaattggccc aggccgcgcc agtgtcgaag
    22681 gcatggcttt cgctgggcgc gggcacgcca tcgcgcgggg ccagcgtttc cgacaccacc
    22741 gccaccagcg ccgaggcgcg gcgcgcccat tgccggttgc gctcgaacag gatggcgagg
    22801 aaatcctccc acgccgcctc gccggggcgg gcaacgatga agcgccaggg ctgcaggttg
    22861 cgcgccgaag gcgcccagcg ggccgcctcg aacacgcttt gcagcacgcc atcgctcacc
    22921 gtctcgccgg tataggcgcg cgggctccag cgctgggtga acagcgggtg gatgggatga
    22981 tcggcgccgc gccgggcggc atcagcaggg gcagtggcag cagcagaggc ggtcatcagc
    23041 ggtcctcccg gggggcaaag ggcaggagat tggccggcgc gccatccgcg cgggcggcag
    23101 gctcgggcgc ggcggtcagc tcggccagga tctcgtcctt cagccggtgc agcagcggcg
    23161 agcggcgatc gcgcgggtgg ggcagatcca cctcgacaat gcggcggatg cggcccgggt
    23221 tgggcgccat gacgatcacc cggtcgccca gatagagcgc ctcctccaca tcatgcgtca
    23281 ccatcaccat ggtcgatcgc tgggccagcc agatgcgctg cagctcgttc tgcacatgca
    23341 cccgggtcag cgcgtcgagc gcgcccagcg gctcgtccag cagcagcagg cgcggctctg
    23401 tcaccagcgc gcgggcaatc gccgcgcgct gcgccatgcc gcccgacagc tggtgcggat
    23461 aggccttttc aaagccgcca aggttcacca gcgcgatatg ttcggcaacg atctggcggc
    23521 ggcgctcctt cggcgtgccg gtgttcagca gggccagctc gatgttctgc tccagcgtca
    23581 tccacggaaa caggcggtga tcctggaaga cgatgccgcg ctccagcccg gtggtcgcca
    23641 cccgctcgcc atcgaggcgg atctcgccgg cataatcggc atccagcccg acgatcagcc
    23701 gcagcagcgt ggatttgccg caacccgagg cgccgacgat gctgacgaat tcgcccggcc
    23761 gcaccgccag attgatgttc gaaaggatct ggcgggggcg cccgccgatg gcaaagctct
    23821 tgtcgagatc ggtgatggtg agcgcgccgc gcgccagcgg agcaggcgcc ggcgcggcgg
    23881 aggaggacag caggaaggga tcgaccatcg gggaagctcc gggtttcaaa agggggcggc
    23941 ctgccagcgc agcaggtggc ggcgcagcag caccacggcc tggttcagcg caaagccgag
    24001 gaaggcggtc aacgcgatcg ccacgaggat gatgtcgagc cgctgctggg tttcggccag
    24061 ctgcatcgtc acccccaggc cgccgccggt gccctgcagg atctcggtgc cgatcgccgc
    24121 gatccaggtg aaggccgccg cctgcgtcag cccggtgagc agcagcggca gcgccgccgg
    24181 cagcagcacc agccgcagcc gctgcaccgg cgaaaagccc agcacccgcg ccacttcgag
    24241 atagcgcggc tccacctgcg caatgccggc atgggcgttg agcatggccg ggaaaaaggc
    24301 cgacaggccg atcaccacca gcgccgcggc atcgcccgtg cccagccaca ggcccaccag
    24361 cggcagccag cccaccatcg gcacctgccg cagcgcgttg agcagcggcg agagcaaccg
    24421 gtcgagcagc ggcagcacgc ccagcgccac gcccagcccc agccccaggc tggcccccag
    24481 cgccagcccg gccagcgcgc gcagcaaggt ggcggcggtg tcggccagca gggtgccctg
    24541 ggcgatcagc tcgcccagcg cgccgcccag cgaggcgagc gaggcaaagg cggcggcatg
    24601 ggccccgcca aggctggcct gccactgcca ccacagcgcc agcagcgccg gcgccaccag
    24661 ccccacggcc aggcgcaccg gccacctgtc gatcaacggc gcgctcatgc gtgcctccag
    24721 cgggtggcgc gggcctcgat ctggcgggcg ccctgatcca gcaggaagcc caccagcccg
    24781 ctgacaacga caccggccag caccacgtcg atctggaaca gctggcggcc catctccatc
    24841 atctggccaa tgccgctgtc ggccgccagc agctcggcgg ccaccagcac gatccaggcg
    24901 cgggtgaggc cgatccgcag ccctgtcagc accgccggca cagtggccgg caccagaatg
    24961 cgccacacca gatcgccaag ccgcgtgcga tagatccgcg ccacatcgaa ccaggcacgc
    25021 ggcagggcgc ggatgccgtc gaacgtggcg agcgccaccg ggaagaaggc cgaaacggcc
    25081 accacggtga tcttgaaggc ctcgtcgatg cccagaaaca gcaccagcac cgggatgaag
    25141 gcgatcaccg gcacctgcca cagcgccaga aacagcggcc acagcgccgc ctgcacccag
    25201 cgcgacaggg ccgtggccgc gccaaaggca aggccggcca cagcgcccag cgcaaagccc
    25261 agcagcagcc ggtgcaggct gtcgcccaga tggcgggcca gctcgcccga ggcggccatg
    25321 cccgccagcg tcgccgccac ccgcgccggc ggcgccagca cctgcggcgg caccagccca
    25381 agccccgcca ccagctgcca caggcccagc agcgccagca ccggcaccag ccgggccgcc
    25441 gcccggcgca ggggcaacag ggcccggctc acgcggcgct atccttgtcg gaccagaagc
    25501 cggtgaggcc aagatcgcgg atcgcctgcg ccacgaagcg cggctcgatc agctggccgg
    25561 tatcgaccgg cgcgtggatc agcccatggg ccagcgcata atcggccacc gagcggtaat
    25621 ggctggcgat cgccgggcgg aacagcggcg agaagcgctc gcgccagctg tagggcgtgc
    25681 tggcataatc ggcctcgata atcggcgtgg gcatggaccc gcgcgcggca ttggcgatca
    25741 cctgcgcctt gttggcctca tccgcgctcc agcgggccgc gcgcacaaag gcgctcacca
    25801 ccagctgggt gatctcggga tgatcggcca cgaaatcgcc gcgggcgaag agttcggcgc
    25861 gcatcttcca gtcggccggg gcctgcttgg tcgaccagat cacccggccg atgcccttct
    25921 gctccagcag cagcccgtct gacagcagca ccgttgcatc cacgctgccg ccggccagcg
    25981 cggcgggggt ggcctgcggg ttgatgttga cgatggtgaa atcggcaaac gtcagcccgg
    26041 ccgaatccag cagcttggag aaaggcagtt cccacggccg cccgcgatgc agggcaatgc
    26101 gcttgccctt gagatcggcg atggagcgcg ccggcagatc cttgcgcacc accagataga
    26161 catcctgccc ctgcccc
//
LOCUS       DASRYE010000191        16581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_11393,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000191 DASRYE010000000
VERSION     DASRYE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11393"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFF98_10025"
     CDS             complement(<1..575)
                     /locus_tag="VFF98_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZU64009.1"
                     /translation="MGKKKHASNARQEHHPVDLEKLQISLSAMQRRLIAEGTRLLVIF
                     EGRDAAGKDGTIKRIVEHMSPRDTRVVALGVPSEHDRRAWYFQRWCAHLPVAGEVALF
                     NRSWYNRAGVEPVMGFCTAAEHEEFLATVPIFEQLLVHSGIKVVKYYLDISRKEQKKR
                     LAERLADPLKQWKTSAVDEAAQKKWKAYTRAR"
     gene            complement(582..2201)
                     /locus_tag="VFF98_10030"
     CDS             complement(582..2201)
                     /locus_tag="VFF98_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZU64010.1"
                     /translation="MLHKAPAAPPRPDCETEIKLVATSAMLRRLRRLPLLGAASQAGA
                     VLQRSTYFDTLAGSLSLAGASLRLRERAGKHEQTLKLAQAGGLERAEWNIPLPADAGT
                     AQPDLAALPPRARTALLAAVGAHPLRPFARVEVMRETRRVTYAGAVIELAFDTGRIVP
                     LTGCGRHGPGTRICELELELVSGRLEAVLALAASLPLGPDLMWQVAGKAERAHALAYD
                     RPLRAARAMPLALSPDMPAGDAFRAIGWNALVHLLANLPLVASDPEAVHQARVALRRL
                     RAAFALFAPLWADGDGSASSASAGPGPAHWRAALGDLARALGPARDLHVLGARLATAP
                     ATPAMARLRQHLAACETQASARAASHIASAPTQALLMALAGWLESPAALAAPAARQPL
                     MAFAGPLLARRRRHLRGQRKQLAALPEASLHRLRIQVKKLRYAVGFLAPAQSTGGSAR
                     AARRHEGVLARLQEVLGGLNDLAVAARPQGPLSAALAEGLPPAEAADMARLLEAACRA
                     EAPRRKALLEEARRLLAEEKRIACWWQAQKE"
     gene            2543..3418
                     /locus_tag="VFF98_10035"
     CDS             2543..3418
                     /locus_tag="VFF98_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU64011.1"
                     /translation="MRSSRLVLMPALALLMVAGTAPAALAADATIDQVYSAARAGQVD
                     QALSLMQPVLKDHPNSAKAHYVEAELLARQGKIDEARVEFSRAQQIAPGLPFASAHSV
                     SELKRQLQGGHAAAAVPAAGDTAPASQGHFPWLPVIVIGGLLLGLLALLRRRRPVQEV
                     YVGQAGGPGYGAGYGGPGYGPQGGPGYGAPGYGGPGYGAPQGGMGSGIMGGLAQGAAM
                     GAGFAAGERVVDGLFGGHDREREREAPARQDGGWDQGGGNAGFGGNTDMGGDDFGISD
                     SGSWDDSSGGGNDGW"
     gene            complement(3443..6781)
                     /locus_tag="VFF98_10040"
     CDS             complement(3443..6781)
                     /locus_tag="VFF98_10040"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZU64012.1"
                     /translation="MYRQALHTPSSRVKLLVAIFIVTLAENQLGQQGLVLRTAIIGSS
                     LGLAGLISVPRNCWLTFALGILAIRVVALFAAGMAPTLALLLAVGVTVQIALASLFLR
                     WRLKDGLDLTRAGQLLDLALAGLIVAPAMGLMVDVLFRRISLAQAANPRTLWQYFSTH
                     DGIITLADWVMPHVLGMVVVVPLALAAITRGIRHPLEWLSRERGALYALLLLAHLLVF
                     SQANAQMLMLVPPALALIAIRLGIRETLLAMLLSMGIASVATANGLGAVAAIYADPPH
                     QISFLRHFYIFAFLCFMPVAATLERNRRLELSLARNTRFTDQILRGMQEVVFRTDMQG
                     RWSFLNPAWLDLTGYSVAESIGTAMRALVSAEGHAEFDEALALLMADDAPEAKLSLDM
                     RRRDGTLRECEVTLRPLRHADGHIAGLGGILRDITEARRHLRALESSERRFRDLCDTA
                     PVGILRTDLAGQITYVNATCELTDLARCDRLLGRALGDWLLGTEGFAPAALARQLGVP
                     AARIEREAEIRDNAGERRWLTVVVTSDFDAAGQQQGYVAAFADITSRKQAELALAEKS
                     AELTTMAENISDVIFRITFDGLCLYATPSVLDLTGLPAERLVGTRTIDRVHPEDRPRM
                     GQALARMARGEVDFVSISYRARADGSRSGYLWHEAHCRLVRDGQGQPAEVIASVRDID
                     ERKRLELELIEARARAEAAATAKSTFLANISHEIRTPMNGVIGLTDLLLERELDATTR
                     QYVQLIADSGATMMHLLNGVLDLAKLEADRLVLADEVIDIRELLAGALALMTAPARAK
                     ALETRLDIAADLPPLMRGDRLRLRQVLANLLGNAVKFTDAGHVALCARTQGAELVICV
                     ADTGMGIAPEAQAAVFEEFVQATAGRPGGTGLGLPISRRLIEAMGGTLTMASAPGEGT
                     AMTIRLPARFMEDRRAAAPAPARPATPVAEAGAQLHILVAEDNRTNQIIASGMLKRLG
                     HRHAIAGNGAEAVARVAEARAAGDPYDLVLMDMLMPEMDGLEATRALRAQGFGAAELP
                     IIAVTANAFAEDVARCLEAGMQAHLAKPLQLAPLGEALELAARVRATGDGSLWALPRE
                     QDRRQTGPALTATDALATDGAPPA"
     gene            complement(6795..8252)
                     /locus_tag="VFF98_10045"
     CDS             complement(6795..8252)
                     /locus_tag="VFF98_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU64013.1"
                     /translation="MARTKILVIEDLSSLAIAYAAHLEKAGYEAVIVENGEDALRALD
                     RQGPFGAILLDLQLPDVDGLDLVKTHPEVLAACPVVVATADASLGRAIEAMRLGAFEF
                     LVKPVAAKRLLAVVGSAVELGRTRPAAPAPVPSAKANERPGTFIGSSPAMREVYRQIE
                     CVAGSRATVFITGESGTGKEVCAEAIHRESGRGRGPFVAVNCGAIPENLLESELFGHL
                     KGSFTGAVSDRMGAVQAAHKGTLFLDEICEMALPLQVKLLRFLQTGTVQRVGSNRVEE
                     VDVRIVCATNRTPEREVAEGRFREDLYYRLAVVPLHMPPLRDRAGDVAELAEAFLQRF
                     AAEERKSFQPLGPEHIAALDAYAWPGNVRELQNVIRRAAVLFEGPELPLRALPAVPPA
                     ATTTPAMAVAGTPAIAGALLQPAPAAADAAAPNRQLLATALAGLTLDEIERIAVDLAV
                     EAAGGSLPAAARVLGVSPSTLYRKRERWLGIAQTA"
     gene            8538..9761
                     /locus_tag="VFF98_10050"
     CDS             8538..9761
                     /locus_tag="VFF98_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64014.1"
                     /translation="MAVLTNRESRRRGRGAGLLLAGALALQGCATMPTPHNIADAGYQ
                     VTQQTGRSATVGRCTRPVVPDGLLAGRRQVSADALADAPDILSPGDRLRLDIAGDHDL
                     LTGFYVVAANGNVVVGQDIVLHAAGRALPEVAAELRARLLEAGLVRDVASNVRLQLAA
                     ASSITVSVEGAVFAPGFVAAGERTDPGRLAVVDHPAMGDYLGNRTLTGAIRSASGIRP
                     DASVQAIYLLRGPTYAVIDETTAMTGGVLMDPQLAAGDRVVVLSVGCFQPDLVKPTAL
                     TQPGIRVFMSNLTRPAASNSASAIGKDTTSLPYGTRFLQALVTANCVGGAMINAQRHA
                     VLISRNPFNGHSVVIQRSIEQLVRDANRDDIDPYLMPNDALACYDSTGESIADVINLL
                     GNLATPAALVKSLSN"
     gene            9758..11197
                     /locus_tag="VFF98_10055"
     CDS             9758..11197
                     /locus_tag="VFF98_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64015.1"
                     /translation="MSNAPLSPAGFAESTAPPRRLKRVLKAARFGLPSVGRYRRYAVV
                     LLPGLAAIWGLTLAYLLFAPVSYKSDFTLILPGSGAGSSINLETIGQAQNTSNSAFAS
                     PTLSPTENYKQLLTADITLDAAMKLAHLAAGRFPAPTVKLVEQTNLIEVSITGKSAKG
                     AQANAEALRQAFLDRLDALRADEAAKRTQSDLVHLQELAAKERLTERKLIDFQARHGL
                     ATLEQFNARIAAVDQLRARERDLRLELRQHQGQTRQLASSLHASPRTANLAWRLRGDP
                     VFQELVMQYAKANADAELKAGTLGARHAAMAQANAERAQLLNAVLRRAQKLTDITRAD
                     LLGVIDLTVADGRSALLQTMDMGDAQATGTADELSELHGDLDKAAASTPDLIEQAQQL
                     ADLQRDHRVAEAVFSSALARLDTNKLDPFASYPLVQTLAAPSLPPKKSSPSLLLGLAG
                     GMAASLLLFISCGLLWLRQPILDKMLRKG"
     gene            11152..12531
                     /locus_tag="VFF98_10060"
     CDS             11152..12531
                     /locus_tag="VFF98_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HZU64016.1"
                     /translation="MAAPAYPRQNASERLITFTIGATYWLWLIGGVYMAGPVLGWVLA
                     YRAARAYYLAPALPAEERPDPLPLVLWAWLLCMLAMEGVLLAGHLNFELGVGQTIKSS
                     VGWAKGWALLGLFPVAGFALPIRLSILVRAVCRLARQTLILLPLLFAAPYIHLPSRLW
                     VSPLKVLGGSGDEFFTVMLYTIDPETHTARWQFFAPWAPAVGMVAVIHFLLAREEADV
                     RWRWAGYAAAVIMAFASSSRMALIALAIMIPITVLVGKLNRPLTWALAAPVALVGAWA
                     LPAIKAASDAAQESFKGARAGSSRVRSALGRIAVQRWQHEAYWCGHGIVERGPHLVEY
                     MPIGSHHTWYGILYVKGLLGAISLAIPMIGTLLAMLWAALRDRAGQLGLAMILTYWFY
                     SFGENLEVLTYIAWPALLTIGIVLRMQVRGVWLPSDKAAPEGAFIDNPYAKAGPPRPR
                     AKGPAPAPA"
     gene            complement(12538..13575)
                     /locus_tag="VFF98_10065"
     CDS             complement(12538..13575)
                     /locus_tag="VFF98_10065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU64017.1"
                     /translation="MKRPRLVNLLDDFALGGVTRGLGIFDCDAVRAVVDPQVERIAKD
                     AWLAPRLEAEVIIVHFPPNWRRLLFLASLRQRNPRARIIWVEHSYTRAWEALKVPEQG
                     RFRLLLRATCRMVDHIVCVSHGQARWLAEAANLSPETVEVIHPWSRDQGLASLPYPAF
                     AAGRPLRVGALGRFAEQKGFDTLISAHRRGLMPGTELVIGGTGPDEERLKLMAGDSPF
                     IHFLGKITDVAGFLEQADVVALPSRWEAYGQVANEAREGGRPIITAPVDGLPEQVGEA
                     GMVVDFTDDAAVLDTFARLRAADLPAMARAARQSTLGCGPLRQQQWAHLLDRALSHAT
                     RGHFGELALAL"
     gene            complement(13572..14573)
                     /locus_tag="VFF98_10070"
     CDS             complement(13572..14573)
                     /locus_tag="VFF98_10070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU64018.1"
                     /translation="MDTPVVPTVSVIMPVYNVEPYVAEAITSVLAQTYADFELIIVDD
                     GGQDRSVEICQGFEDPRIRIVHQANRGLAGARNTGILAARGRYLAFLDSDDRWLPEKL
                     ALHVIHLEANPDVGVSFSPSRFIDVNGAPMRLRMKPKLDGISPEDIFCRNPVGNGSAP
                     VIRRTALEAIAFRHPTEPGRTCFFDESLRQSEDIECWLRLALIGKVRFAGIAPALTEY
                     RVAGGGLSAQVLRQYETWQGVVDRLQAYAPEFAARHLPRARAYQLRYLARRSVQLGDA
                     GMAMAMMKEALKSSRKPLLEEPVKSLTTLTAAWVGRMMKPERFARLASLLSGGRLVA"
     gene            complement(14614..15594)
                     /locus_tag="VFF98_10075"
     CDS             complement(14614..15594)
                     /locus_tag="VFF98_10075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU64019.1"
                     /translation="MPIPADRPTDASRPAPQAGAVEVSVVMPVYNAEATIEATVRSVL
                     AQTMPRFELIAIDDGSRDGSLAVLKRLAGEDGRLKIIAQGNAGVSAARNLGAELAQGE
                     LIAFLDADDLWAPEKLAAHAMLHREEPDLVASYARIAFIAADASGLEGAATTSSLCPH
                     TPGLTDVLGENPVCTASNFVVRREAFLAAGSFDRALSFAEDQEMVARLIQRGGRLEGI
                     DAVLTGYRFSPGGLSMALERMYDGWRKVAARYLSAAEMAPLEALYCRYLARRVLRAGG
                     RPAEALRYVARGLRLDAHAFMAQRRRALGTIAAALAAPALPPALRQRLFA"
     gene            15905..>16581
                     /locus_tag="VFF98_10080"
     CDS             15905..>16581
                     /locus_tag="VFF98_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZU64020.1"
                     /translation="MSVHAAHWDLPAARPLTAADAAAAQRSAFTLPRQLHDHERTTLF
                     GLPLIKGERDHVASEIVAMAKARECSTIQFINAHCVNVAAHDAAYREALGAADFLLPD
                     GSGLAIAARMAGLELGENLNGTDLFPDLCRHAAAAGLSIYLLGGKPGIATAAAKTMLQ
                     RYPDLVIAGTRHGYWTDEEEPALIEEINDSGAALVFVGLGVPRQEKWIARVRPELRAR
                     IVLGVGGL"
BASE COUNT         2520 a         5669 c         5826 g         2566 t
ORIGIN      
        1 cgcgcgcggg tgtaggcctt ccacttcttc tgcgcggcct cgtccaccgc cgatgtcttc
       61 cattgcttga gcggatcggc cagcctttcg gccaggcgct tcttctgctc cttgcggctg
      121 atatcgaggt agtatttcac caccttgatg ccggaatgga ccagcaactg ctcgaagatc
      181 ggcacggtgg cgaggaattc ctcatgctcg gccgccgtgc aaaagcccat caccggttca
      241 acgcctgcgc ggttgtacca gctgcggttg aacagcgcca cttcgcccgc caccggcaga
      301 tgcgcgcacc agcgctggaa ataccacgcc cgccggtcat gttcggatgg cacgcccagc
      361 gccaccacgc gggtatcgcg cgggctcata tgttcaacga tgcgcttgat cgtgccatcc
      421 ttgccggcgg catcgcgccc ctcgaagatg accagcagcc gcgtgccctc ggcgatcagc
      481 cggcgctgca tggccgaaag gctgatctgc agcttttcca gatccacggg atgatgttcc
      541 tgccgggcgt tgctggcatg cttcttcttg cccaagggcg ctcactcctt ctgcgcctgc
      601 caccagcagg cgattcgctt ctcttcggcc agcagccggc gggcctcttc cagcagggcc
      661 ttgcggcggg gggcctcggc gcggcaggcc gcttccagca ggcgggccat gtcggcggcc
      721 tcggcagggg gcagcccttc ggccagggcc gcgctcaagg ggccttgcgg gcgcgcggcc
      781 accgccagat cgttcagccc gcccagcact tcctgcagcc gcgccagcac gccctcgtgc
      841 cggcgggccg cccgcgcgct gccgccggtg ctttgcgcgg gggcgagaaa acccaccgca
      901 taacgcagct tcttcacctg gatgcgcagg cggtggaggc tggcctcggg cagggcggca
      961 agctgcttgc gctgcccgcg cagatgccgg cggcggcgcg ccagcagggg gccggcaaac
     1021 gccatcagcg gctggcgggc ggcgggcgcc gccagggcgg cggggctttc cagccagccg
     1081 gccagcgcca tcagcagcgc ctgggtgggg gcagaggcga tgtggctggc tgcgcgcgcg
     1141 ctggcctgcg tctcgcaagc ggccagatgc tggcgcagcc gggccatggc gggcgtggcc
     1201 ggcgcggttg ccagccgggc gcccagcaca tgcagatcgc gcgccggacc cagcgcgcgg
     1261 gccagatcgc ccagcgccgc gcgccagtgg gcagggcccg gcccggcact ggcactactg
     1321 gcgctgccat cgccatccgc ccacagcggc gcgaacagtg cgaaggccgc ccgcagccgg
     1381 cgcagcgcca cccgcgcctg atgcaccgcc tcgggatcgc tggccaccag cggcaggttg
     1441 gccagcagat gcaccagcgc gttccagccg atggcgcgaa aggcatcgcc ggccggcatg
     1501 tcggggctca gcgccagcgg catggcgcgg gcggcgcgca gcggccggtc ataggccagg
     1561 gcatgggcgc gctcggcctt gccggccacc tgccacatga gatcgggccc cagcggcagg
     1621 ctggcggcca gcgccagcac cgcctccagc cggcccgaca ccagctccag ctccagctcg
     1681 cagatgcgtg tgcccggccc atgccggccg caaccggtga gcggcacgat gcgcccggtg
     1741 tcgaacgcca gctcgatcac cgcgccggca taggtgacgc ggcgggtttc gcgcatcact
     1801 tccacccgcg cgaaggggcg cagcgggtgc gcgcccaccg cggccagaag cgcagtgcgc
     1861 gcgcgtggcg gcagggcggc cagatcgggc tgggcggtgc cggcatcggc gggcaggggg
     1921 atattccatt ccgcccgctc cagcccgccg gcctgcgcca gtttcagcgt ctgttcgtgc
     1981 ttgccggccc gctcgcgcag gcgcagcgag gcgcccgcca ggctgaggct gccggccagc
     2041 gtgtcgaaat aggtggagcg ctgcagcact gcgccggcct ggctggctgc ccccagcagc
     2101 ggcaggcggc gcaggcggcg cagcatggcg ctggtggcca ccagcttgat ctcggtttcg
     2161 caatcggggc ggggcggggc ggccggtgcc ttgtgcagca cgggcgggtg aggggcggca
     2221 ggccttggcg cggcgcgggc cccggcacgc cgcgcagccg ccttgggcaa ggcttgcgga
     2281 aggggcgctt gtgccatcac gcgttctcct gtggcgcctg cccgggcaag gtctatccag
     2341 cggccggcaa gactaggcag gcatggcgct tgcggcaagg cctgtcgttg atctggcgca
     2401 tcagggattt tacggggggc ggggaggggg cgcaaggggg gaggcgcaag gggccccgct
     2461 gccctcaggc cgggttcatc ttgcaaatgg cacagggttc tcccacatcg cgcgcgcacg
     2521 cttttgtgct ggagagatag tcatgcgaag ttcacgcctt gtcctgatgc ctgcgctggc
     2581 gctgctgatg gtggcgggca ccgcgccggc ggccctggcc gccgatgcga cgatcgatca
     2641 ggtgtattcc gccgcccgtg ccgggcaggt ggatcaggcg ctgagcctga tgcaaccggt
     2701 gctgaaggac caccccaaca gcgccaaggc ccattatgtg gaagccgaat tgctggcccg
     2761 ccagggcaag atcgacgagg cgcgggtgga attttcccgc gcgcagcaga tcgccccggg
     2821 cctgcccttt gccagcgccc attccgtctc ggaactgaag cgccagctgc agggcggcca
     2881 tgccgcagcg gccgtgccgg cggccggtga cactgcgcct gccagccagg gccatttccc
     2941 ctggctgccg gtgatcgtga tcggcggcct gctgctgggc ctgctggctt tgctgcgccg
     3001 ccgccggccg gtgcaggaag tgtatgttgg ccaggcgggc gggccgggct atggcgcggg
     3061 ttatggcggg ccgggttatg gcccccaggg tggccccggc tatggcgcgc ccggttatgg
     3121 cgggcccggt tatggcgcgc cgcagggcgg catgggctcg ggcatcatgg gcgggctggc
     3181 gcagggcgcg gcgatgggcg cgggctttgc cgctggcgag cgcgtggtcg atggcctgtt
     3241 cggcggccac gaccgtgagc gcgaacgcga ggcacccgcc cgccaggatg gcggctggga
     3301 ccagggcggc ggcaatgccg gctttggtgg caacaccgat atgggcggcg atgatttcgg
     3361 catttccgac agcggctcgt gggatgattc ctcgggcggc ggcaacgacg gctggtaaac
     3421 cccggcgcgc gcccttcccg cctcaggccg gaggggcgcc atcggttgcc agcgcgtcgg
     3481 ttgctgtcag cgcggggccg gtctggcgcc ggtcctgctc ccgcggcagg gcccacaggc
     3541 tgccatcgcc ggtggcgcgc accctggcgg ccagctccag cgcctcgccc agcggggcca
     3601 gctgcagggg cttggccaga tgggcctgca tgccggcttc caggcagcgg gccacatcct
     3661 cggcaaaggc attggccgtc acggcgatga tcggcagctc ggccgcgcca aagccctgcg
     3721 cgcgcagggc acgggtggct tccagcccgt ccatctcggg catcagcatg tccatcagca
     3781 ccagatcata gggatcgccg gcggccctgg cctcggccac gcgggccacc gcctcggcgc
     3841 cattgccggc gatggcatgg cgatggccca gccgcttcag catgccgctg gcgatgatct
     3901 ggttggtgcg gttatcctcg gccaccagaa tgtggagctg ggcgccggcc tcggccaccg
     3961 gcgttgccgg ccgggcgggc gcgggggcgg cggcgcggcg gtcttccatg aagcgggcgg
     4021 gcaggcggat ggtcatggcg gtgccttcgc ccggcgcgct ggccatggtg agggtgccgc
     4081 ccatcgcctc gatcagccgg cgcgagatgg gcaggcccag cccggtgccg cccggccggc
     4141 cggcggtggc ctggacaaat tcctcgaaca cggccgcctg cgcttcgggg gcgatgccca
     4201 tgccggtgtc ggccacgcag atcaccagct ctgccccttg ggtgcgggcg cacagggcca
     4261 catggccggc atcggtgaat ttcaccgcat tgcccagcag attggccagc acctggcgca
     4321 ggcgcagccg gtctccgcgc atcagcgggg gcaggtcggc ggcgatgtcc agccgggttt
     4381 ccagcgcctt ggcgcgggcg ggtgcggtca tcagcgccag cgcgccggcc agcagctcgc
     4441 ggatgtcgat cacctcgtcg gccagcacca gccggtcggc ctccagcttg gccagatcca
     4501 gcacgccgtt caacaggtgc atcatggtgg cgccgctatc ggcgatcagc tgcacatact
     4561 ggcgggtggt ggcatccagc tcgcgttcca gcagcaggtc ggtcaggccg atcaccccgt
     4621 tcatcggcgt gcggatctcg tgactgatgt tggcgaggaa ggtgctcttg gcggtggcgg
     4681 cggcctcggc gcgggcgcgc gcctcgatca gttccagctc cagccgcttg cgctcgtcaa
     4741 tatcgcgcac cgaggcgatc acttcggccg gctggccctg gccatcgcgc acgaggcggc
     4801 aatgggcctc gtgccacaga tagccgctgc gcgagccatc ggcccgggcg cgatagctga
     4861 tcgagacgaa atccacctcg ccgcgggcca tgcgggccag cgcctggccc atgcgggggc
     4921 ggtcttcggg gtgcacgcgg tcgatcgtgc gggtgcccac cagccgttcg gcgggcaggc
     4981 cggtgagatc cagcaccgaa ggggtggcat acaggcacag gccatcgaag gtgatgcgga
     5041 agatgacatc gctgatgttc tcggccatcg tcgtcagctc ggccgatttt tccgccagcg
     5101 ccagttcggc ctgcttgcgg ctggtgatat cggcaaaggc ggcgacatag ccctgctgct
     5161 ggccggcggc atcgaaatcg ctggtgacaa ccaccgtcag ccagcggcgc tcgccggcat
     5221 tgtcgcggat ctcggcctcg cgctcgatgc gggcggccgg cacgcccagc tggcgggcca
     5281 gcgctgccgg ggcaaagcct tccgtgccca gcagccaatc gcccagcgcg cggcccagca
     5341 gccggtcgca gcgggcaagg tcggtcagct cgcatgtggc gttcacataa gtgatctggc
     5401 cggcaagatc ggtgcgcagg atgcccacag gggcggtgtc gcacaggtcg cggaagcgcc
     5461 gctcgctgct ttccagcgcg cgcaggtggc gcctggcctc ggtgatgtcg cgcaggatgc
     5521 cgcccagccc ggcgatatgg ccatcggcat ggcgcagcgg ccgcaaggtc acctcgcatt
     5581 cgcgcagcgt gccatcgcgc cggcgcatgt cgaggctcag cttggcctcg ggggcatcgt
     5641 cggccatcag cagggccagc gcctcgtcga attcggcgtg gccttctgcg ctcaccagcg
     5701 cgcgcatggc ggtgccgatg ctttcggcca cgctgtagcc ggtgagatcc agccaggccg
     5761 ggttgaggaa cgaccagcgc ccctgcatgt cggtgcggaa caccacttcc tgcatgccgc
     5821 gcaggatctg gtcggtgaag cgggtgttgc gggccagcga cagttccagc cggcggttgc
     5881 gttccagcgt ggcagccacc ggcatgaagc acaggaaggc gaagatgtag aaatggcgca
     5941 ggaagctgat ctgatgcggc ggatcggcat agatcgccgc cacagcgccc agcccgttgg
     6001 ccgtggcaac agacgcgatg cccatcgaca gcagcatcgc cagcaaggtt tcgcggatgc
     6061 ccaggcggat ggcgatcagc gccagcgccg ggggcaccag catcagcatc tgggcattgg
     6121 cctggctgaa caccagcaga tgcgccagca gcagcagggc atacagcgcc cccctctcgc
     6181 gcgagagcca ttccagcggg tggcggatgc cgcgggtgat cgcggccagc gccagcggca
     6241 ccaccaccac catgcccagc acatgcggca tcacccagtc ggccagggtg atgatgccgt
     6301 catgggtgct gaaatattgc cacagcgtgc gcggattggc ggcctgggcc aggctgatgc
     6361 ggcggaacag cacatccacc atcagcccca tcgccggcgc aacgataagg cccgccagcg
     6421 ccagatccag caattgcccc gcgcgggtga gatccagccc gtctttcagg cgccagcgca
     6481 ggaacagcga ggccagcgcg atctgcacgg tgacgccaac cgccagcagc agcgccagcg
     6541 tgggggccat gccggcggca aacagcgcca ccacccggat cgccagaatg cccagcgcga
     6601 atgtcagcca gcagttgcgc ggcaccgaga tcagcccggc cagccccagc gaggagccga
     6661 tgatcgccgt gcgcagcacc agcccctgct ggcccagctg gttttccgcc agcgtgacga
     6721 tgaagatcgc caccagcagc ttcacccggc tggaaggcgt gtgcagggct tgccgataca
     6781 tggctggccc tccctcaggc ggtttgggcg atgcccagcc agcgctcgcg cttgcgatag
     6841 agggtggagg ggctgacccc cagcacccgg gctgcggccg gcaggctgcc accggccgct
     6901 tccacggcca gatccaccgc gatgcgctcg atctcatcca gcgtcagccc ggccagcgct
     6961 gtggccagca gctggcggtt gggcgcggcc gcatcggcag cagccggcgc gggctggagc
     7021 agggccccag ctatggccgg cgtgccggct actgccatcg ccggggtggt ggtggccgcg
     7081 ggcggcacgg cgggcagcgc gcgcagcggc agctcgggcc cttcgaacag cacggcggcg
     7141 cggcggatca cattctgcaa ctcgcgcaca ttgcccggcc aggcataggc atcgagcgcg
     7201 gcgatatgct cgggccccag cggctggaag ctcttgcgct cctcggcggc aaagcgctgg
     7261 aggaaggctt cggccagctc ggccacatcg ccggcgcgat cgcgcaaggg gggcatgtgc
     7321 agcggcacca cggcgaggcg atagtacaga tcctcgcgga agcgcccctc ggccacttcg
     7381 cgctcgggcg tgcggttggt ggcgcacacg atgcgcacat ccacctcttc cacccggttg
     7441 gagcccaccc gctgcacggt gcccgtctgc aggaagcgca gcagcttcac ttgcagcggc
     7501 agcgccatct cgcagatctc atcaaggaac agcgtgccct tgtgggcggc ctgcaccgcg
     7561 cccatgcgat cggaaacggc gccggtgaac gagcccttga gatggccgaa cagttccgat
     7621 tcaagcaggt tttccgggat cgcgccgcag ttcaccgcca cgaagggccc gcgcccgcgc
     7681 ccgctctcgc ggtggatcgc ctcggcgcac acttccttgc cggtgccgct ttcgccggtg
     7741 atgaacaccg tggcgcggct gccggccacg cattcgatct ggcgatagac ctcgcgcatg
     7801 gcgggcgagg agccaatgaa ggtgcccggc cgctcgttgg ccttggcgct gggcacgggg
     7861 gcaggggcgg ccgggcgggt gcggcccagt tccaccgcgc tgcccactac cgccagcagc
     7921 cgcttggccg ccaccggctt caccagaaat tcaaaggcgc ccagccgcat cgcctcgatc
     7981 gcgcggccca gcgaggcatc ggcggtggcc actaccaccg ggcaggcggc cagcacttcg
     8041 ggatgcgtct tcaccaggtc cagcccgtcg acatcgggca gctgcaggtc gagcaggatg
     8101 gcgccgaagg ggccctgccg gtcgagcgcg cgcagcgcat cctcgccgtt ttcgacaatc
     8161 accgcctcat agcccgcctt ttccagatgc gccgcatagg cgatcgccag cgaggaaaga
     8221 tcttcgatca ccaggatttt ggtacgtgcc atcacactgc cccagctgtt caatcccccc
     8281 tgccgccggc ccttgccggg gcgggtagat ctgcccatgt cgcggcccat tatcggggct
     8341 acgtggattt gcgtaatgca gcggaagtta ttaccaattt gcaaatggca aaggaaacgg
     8401 tgggcaaagg cagcaaaatg ggctttggcg ttctgccttt ttcaacgctt tgcgaagtag
     8461 actggctttg gcataagcat tgctgcgtag gcggtaatct gggatgagcc cgcggggcgc
     8521 aacggaatcg ggtgggcatg gcggtgttaa ccaataggga aagcaggcgg cgcgggcggg
     8581 gggccgggct gttgctggcc ggcgcgctgg cgttgcaggg ctgcgccacc atgcccacgc
     8641 cccacaacat cgccgatgcc ggctatcagg taacgcagca gaccgggcgt tcggccacgg
     8701 tggggcgctg cacgcggccg gtggtgcccg atggcctgct ggccggccgc cgccaggtga
     8761 gcgccgatgc gctggccgat gcccccgata tcctctcgcc gggcgaccgg ctgcggctgg
     8821 atatcgctgg cgaccatgat ctgttgaccg gcttctatgt ggtggcggcc aatggcaatg
     8881 tggtggtggg gcaggatatc gtgctgcatg ccgccggccg cgccctgccc gaagtggccg
     8941 ccgaactgcg cgcgcgcctg ctggaagcgg ggctggtgcg cgatgtggcg agcaatgtgc
     9001 ggctgcagct ggcagcggct tcctccatca ccgtgtcggt ggaaggggcg gtgtttgccc
     9061 cgggctttgt ggccgcgggc gagcgcaccg atcccggccg gctggcggtg gtcgatcatc
     9121 cggcgatggg cgattatctg ggcaaccgca cgctgacagg ggcgatccgc tcggccagcg
     9181 gcatccgccc cgatgcctcg gtgcaggcga tctatctgct gcgcgggccc acctatgcgg
     9241 tgatcgacga gacgacggcg atgaccggcg gcgtgctgat ggacccgcag ctggccgcgg
     9301 gcgatcgggt ggtggtgctt tcggtcggct gtttccagcc cgatctcgtc aagcccaccg
     9361 cgctcaccca gccgggcatc cgcgtgttca tgtcgaacct cacgcggccg gcggccagca
     9421 attcggcctc ggcgatcggc aaggatacca ccagcctgcc ctatggcacg cgcttcctgc
     9481 aggcgctggt cacggccaat tgcgtgggcg gggcaatgat caacgcccag cgccatgcgg
     9541 tgctgatcag ccgcaatccg ttcaatggcc attcggtggt gatccagcgc tcgatcgagc
     9601 agctggtgcg cgatgccaac cgcgatgaca tcgaccccta tctgatgccc aacgatgccc
     9661 tggcctgtta cgacagcacc ggcgaatcga ttgccgatgt gatcaacctg ctgggcaatc
     9721 tggcaacgcc ggcggccctg gtgaagagcc tctccaattg agcaatgcgc ctctctcgcc
     9781 cgccggcttt gccgaaagca ccgccccgcc gcgccggctg aagcgggtgc tgaaggcggc
     9841 ccgtttcggc ctgcccagcg ttgggcgcta tcggcgctat gcggtggtgc tgctgccggg
     9901 gctggcggcg atctggggcc tcacgctggc ctatctgctg ttcgccccgg tcagctacaa
     9961 gagcgatttc acgctgatcc tgccgggctc gggcgcgggc agctcgatca atctggaaac
    10021 catcggccag gcgcagaaca cttccaattc cgccttcgcc tcgcccaccc tcagccccac
    10081 cgagaattac aagcagctgc tcaccgccga tatcacgctg gatgcggcga tgaagctggc
    10141 ccatctggcc gcggggcgct ttcccgcgcc cacggtgaag ctggtggagc agaccaacct
    10201 tatcgaggtg tcgatcaccg gcaagagcgc caagggcgcg caggccaatg ccgaggccct
    10261 gcgccaggcc ttcctcgacc ggctggatgc cctgcgcgcc gacgaggccg ccaagcgcac
    10321 ccaatccgat ctggtgcatc tgcaggaact ggccgccaag gaacggctga ccgagcgcaa
    10381 gctgatcgat ttccaggccc gccatggcct ggccacgctg gagcagttca acgcccgcat
    10441 cgctgcggtg gatcagctgc gcgcgcgtga gcgggatctc aggctggaac tgcgccagca
    10501 tcagggccag acccgccagc tggccagcag cctgcatgcc tcgccgcgca cggctaatct
    10561 ggcctggcgc ctgcgcggcg atccggtgtt tcaggagctg gtgatgcaat atgccaaggc
    10621 caatgccgat gccgagctga aggccggcac gctgggcgcc cgccatgccg ccatggccca
    10681 ggccaatgcc gagcgcgccc agttgctgaa cgccgtgctg cgccgcgccc agaagctgac
    10741 cgacatcacc cgcgccgatc tgctgggggt gattgacctg accgttgccg atggccgctc
    10801 ggcgctgctg cagacgatgg acatgggcga tgcccaggcc accggcaccg ccgacgagct
    10861 gagcgagctg catggcgatc tcgacaaggc cgccgcttcc acccccgatc tcatcgaaca
    10921 ggcccagcag ctggccgatc tccagcgcga tcaccgggtg gcagaggcgg tgttctcctc
    10981 ggcgctggcc cggctggata ccaacaagct cgatcccttc gcttcctatc ccctggtgca
    11041 gaccctggcc gccccctcgc tgccgcccaa gaaatccagc ccctcgctgc ttctgggcct
    11101 ggccgggggc atggccgcca gcctgctcct cttcatctca tgcggattgc tatggctgcg
    11161 ccagcctatc ctcgacaaaa tgcttcggaa aggctgatca ccttcaccat cggcgcaacc
    11221 tactggttgt ggctgattgg cggggtgtat atggccgggc ccgtgctggg ctgggtgctg
    11281 gcctacaggg ccgcgcgcgc ctattatctg gccccggcgc tgccggccga agagcgcccc
    11341 gatccgctgc cgctggtgct gtgggcctgg ctgttgtgca tgctggcgat ggagggcgtg
    11401 ctgctggccg gccacctcaa tttcgagctg ggcgtggggc agacgatcaa gtcctcggtg
    11461 ggctgggcca agggctgggc gctgctgggg ctgtttccgg tggccggttt tgccctgccc
    11521 atccgcctgt cgatcctggt gcgggcggtg tgccggctgg cccggcagac gctgatcctg
    11581 ctgcccttgc tgtttgccgc gccctatatc cacctgccca gccggctgtg ggtgtcgccg
    11641 ctgaaggtgc tgggcggctc gggcgatgaa ttcttcaccg tcatgctcta cacgatagac
    11701 cctgaaacgc acaccgcgcg ctggcagttc ttcgccccct gggcgccggc ggtgggcatg
    11761 gtggcggtga tccacttcct gctggcgcgc gaggaggccg atgtgcgctg gcgctgggcg
    11821 ggctatgcgg cggcggtgat catggccttt gcctcctcca gccgcatggc gctgatcgcg
    11881 ctggcgatca tgatcccgat cacggtgctg gtgggcaagc tcaaccggcc gctcacctgg
    11941 gcgctggcag cgccagtggc gctggtgggg gcctgggcgc tgccggcgat caaggcggcc
    12001 agcgatgccg cgcaggaaag cttcaagggc gcgcgggccg gctccagccg ggtgcgttcg
    12061 gcgctggggc gcattgccgt gcagcgctgg cagcacgagg cctattggtg cggccatggc
    12121 attgtggaac ggggcccgca tctggtggaa tacatgccca tcggcagcca tcacacctgg
    12181 tatggcattt tgtatgtgaa ggggctgctg ggggcgatct cgctggcgat cccgatgatc
    12241 ggcacgctgc tggccatgct gtgggcggcg ctgcgtgatc gggcgggcca gctgggcctc
    12301 gccatgatcc tcacctactg gttctacagt ttcggcgaga acctcgaagt gctcacctat
    12361 atcgcctggc cggcgctgct gacgatcggc atcgtgctgc gcatgcaggt gcgcggcgtg
    12421 tggctgccca gtgacaaggc cgcgcccgag ggggccttta tcgacaatcc ctatgccaag
    12481 gccgggccac cgaggccgcg ggccaagggg cccgcaccgg cccctgcctg agatttttta
    12541 aagcgccagc gccagttccc cgaaatggcc ccgcgtggca tgcgacaatg cgcgatcgag
    12601 cagatgcgcc cattgctgct ggcgcaaggg cccgcagccc agtgtcgatt gccgggcggc
    12661 gcgcgccatg gccggcagat ccgccgcgcg cagccgggcg aatgtgtcga gcacggcggc
    12721 atcatcggtg aaatccacca ccatgccggc ctcgcccacc tgttcgggca ggccatcgac
    12781 aggcgccgtg atgatcggcc ggccaccctc gcgcgcttcg ttggccacct gcccataggc
    12841 ttcccatcgc gagggcagcg ccaccacgtc ggcctgctcc agaaagccgg cgacatcggt
    12901 gatcttgccg aggaagtgga tgaaggggct gtcgccggcc atcagcttca ggcgttcctc
    12961 atcggggccg gtgccgccga tcaccagttc ggtgcccggc atcagcccgc gccggtgggc
    13021 gctgatcagc gtgtcgaagc ccttctgctc ggcaaagcgg cccagcgctc ccacgcgcag
    13081 cggccggccg gcggcaaagg ccggataggg caggctggcc agcccctgat cgcgcgacca
    13141 gggatggatc acctccaccg tctcgggcga gagattggcc gcctcggcca gccagcgggc
    13201 ctgcccgtgg ctgacgcaga cgatgtgatc gaccatccgg caggtggccc gcagcaacag
    13261 ccgaaagcgc ccctgttccg gcactttcag cgcttcccag gcgcgggtat agctgtgttc
    13321 cacccagatg atgcgggcgc gcgggttgcg ctggcgcagg ctggccagaa acagcagccg
    13381 ccgccagttg ggcggaaaat gcacgatgat cacctcggcc tccagccggg gggccagcca
    13441 ggcgtccttg gcaatgcgtt ccacctgcgg atcgaccacc gcgcgcacgg catcgcaatc
    13501 gaagatgcca aggccgcgcg ttaccccgcc aagggcgaag tcatccagca ggttgaccag
    13561 gcgggggcgt ttcacgccac cagccttccg ccggaaagca ggctggccag gcgggcgaag
    13621 cgctcgggct tcatcatccg ccccacccag gccgcggtca gcgtggtgag cgatttcacc
    13681 ggctcttcca gcagcggctt gcggctggat ttcagcgctt ccttcatcat cgccatggcc
    13741 atgccggcat cgcccagctg caccgaacgg cgcgccagat agcgcagctg ataggcccgc
    13801 gcgcgcggca ggtggcgggc ggcaaattcg ggggcatagg cctgcagccg gtccaccacg
    13861 ccctgccagg tttcatactg gcgcagcacc tgggccgaaa ggccaccgcc cgccacgcga
    13921 tattccgtca gcgccggggc aatgccggca aagcgcacct tgccgatcag cgccagccgc
    13981 agccagcatt cgatatcctc gctctggcgc aggctttcat cgaagaagca ggtgcggccc
    14041 ggttcggtcg ggtggcgaaa ggcaatcgcc tccagcgccg tgcggcggat caccggggcc
    14101 gagccattgc ccaccggatt gcggcagaag atgtcttccg ggctgatgcc atccagcttg
    14161 ggcttcatgc gcaggcgcat cggcgccccg ttcacatcga tgaagcgcga cgggctgaac
    14221 gagaccccga catcgggatt ggcttccaga tggatgacat gcagcgccag cttttccggc
    14281 agccagcggt catcggaatc gaggaaggca agatagcggc cacgcgcggc cagaatgccg
    14341 gtgttgcggg cgccagcgag gccccggttg gcctgatgga cgatacggat gcgcggatct
    14401 tcaaagccct ggcagatctc caccgagcga tcctggccgc catcgtcaac gatgatcagc
    14461 tcgaaatcgg cataggtctg ggccagcacc gaggtgatcg cctcggcgac atagggctcg
    14521 acattgtaga cgggcatgat caccgaaacg gtgggaacaa caggcgtatc catggcggtt
    14581 tccatctcgt tgcagggggt gaaaggcaag gtgtcaggca aacaggcgct ggcgcagggc
    14641 cggcggcagg gcaggggcgg caagcgccgc ggcaatcgtg cccagcgccc ggcggcgctg
    14701 ggccatgaag gcatgggcat ccagccgcag cccgcgcgcc acatagcgca gcgcctcggc
    14761 cggccgcccg cccgcgcgca gcacccggcg cgccagatag cggcagtaga gcgcctccag
    14821 cggagccatc tcggcagcgc tgagatagcg cgcggcgacc ttgcgccagc catcatacat
    14881 gcgctccagc gccatggaga ggccccccgg gctgaagcga tagccggtga gcacggcatc
    14941 gatcccttcc agccgcccgc cgcgctggat aaggcgggcc accatctcct gatcctcggc
    15001 aaaggacagc gcgcgatcga agctgccggc ggccaggaag gcctcgcgcc gcaccacgaa
    15061 attggacgcg gtgcacaccg ggttctcgcc cagcacatcg gtcagccccg gcgtgtgggg
    15121 gcacaggctg gaggtggtgg ccgccccttc cagcccgctg gcatcggccg cgataaaggc
    15181 gatgcgggca tagctggcca ccagatccgg ctcctcgcgg tgcagcatgg catgggcggc
    15241 cagcttttcc ggcgcccaca ggtcgtcggc atcgaggaag gcgatcaact cgccctgcgc
    15301 cagttcggcg cccagattgc gcgcggccga aacgccggca ttgccctggg cgatgatctt
    15361 cagccgcccg tcctcgccgg ccagccgctt cagcacggcc aggctgccat cgcgcgagcc
    15421 atcgtcgatg gcgatcagct cgaagcgcgg catcgtctgc gccagcaccg agcgcacggt
    15481 ggcctcgatc gtcgcctcgg cattgtagac cggcatgacg acagagactt ccaccgcacc
    15541 cgcttgcggc gcggggcggc tggcatcggt gggcctgtcg gcgggaatgg gcatgtggcg
    15601 ctcctgcggg ggatcggatg ccagaatggt aagcacacgc tgtgccaaga tcggcaagcg
    15661 gcgcaaaagc gccattttgc cgtctggcgc gggggcgcat ccgcgcgcgg gggcgccggg
    15721 atgtgcattt tgcatcgcag gatgcaaaac cgcggcagag ggctttgcgc aatgggatga
    15781 tcgccgcgca agggggatgg gctcacttgc cattttgcga aagtccttgg cagaaatggc
    15841 gcaattccgc gattggcacg ggctgtgctg cagcaatggc aaaccgtttc cgaaaggatg
    15901 agccatgagc gtgcatgctg cccactggga tcttcccgcc gcccgcccgc tgacggcggc
    15961 cgatgctgcc gctgcccaga ggagcgcctt caccctgccg cgccagctgc atgatcacga
    16021 gcgcaccacc ctgttcggcc tgccgctgat caagggcgag cgcgaccatg tggccagcga
    16081 gatcgtcgcc atggccaagg cgcgcgaatg ctccaccatc cagttcatca acgcccattg
    16141 cgtgaatgtg gccgcccatg atgcggccta tcgcgaggcg ctgggcgcgg ccgatttcct
    16201 gctgcccgat ggctctggcc tggcgattgc cgcgcgcatg gccgggctgg aactgggcga
    16261 gaacctcaac ggcaccgacc tgtttcccga tctgtgccgc catgcggcgg cggccggcct
    16321 gtcgatctat ctcctgggcg gcaagcccgg catcgccacg gcggccgcga agacgatgct
    16381 gcagcgctat cccgatctcg tcatcgccgg cacccgccat ggctactgga ccgatgagga
    16441 ggagccggcg ctgatcgagg agatcaacga ttcgggcgcg gcgctggtgt ttgtgggcct
    16501 gggcgtgccg cggcaggaga agtggatcgc ccgggtgcgc cccgagctgc gcgcgcgcat
    16561 cgtgctgggc gtgggcgggc t
//
LOCUS       DASRYE010000192         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_26400,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000192 DASRYE010000000
VERSION     DASRYE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26400"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFF98_10085"
     CDS             complement(<1..776)
                     /locus_tag="VFF98_10085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU64021.1"
                     /translation="MCATRSAAASAKASRNIAALDLLRWCSAALVWAYHAGAAYLAAP
                     SAVSRNALTGVSLPRMLVGYSWFGWIGVEIFFILSGYVIARSAEGSAPGAFIRRRALR
                     LLPAAWLCASLSAACVLVAGLEKPHIILDEWAASAFFLSPLDQIDGSWWTLGLEVDFY
                     LLVALCLWGGRRWPLRWLPAALTLACAGFWLYRWMGHSAPRDQWGRYTDLALLRHGAF
                     FALGMLMERRLTGGLRWPDWLTAAAALGACAYEIYDHADER"
     gene            881..2266
                     /gene="radA"
                     /locus_tag="VFF98_10090"
     CDS             881..2266
                     /gene="radA"
                     /locus_tag="VFF98_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZU64022.1"
                     /translation="MAKPKRRFVCSACGGVSSRWQGQCADCGEWNTLAEDAPQTVFSV
                     KHNLSSGGRAFAFEPLDAAVPPLARASTGLAEFDRALGGGLVPGSAILMGGDPGIGKS
                     TLLLQASAAVAQRGGVAVYISGEEAPGQVRLRAERLGLAKAPIRLAAATSVRDILTTL
                     GQMEPPELLVIDSIQCMHSDTIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTK
                     DGSIAGPRVLEHMVDVVMAFEGERSHQYRILRNLKNRFGPVDEIGVFAMEGAGLAEVA
                     NPSMLFLSGREEQVAGSAVFPAMEGTRPVLVEIQALIVRLQSGATPRRAVVGWDNGRL
                     AMLLAVLEARCGLNFSSAEVYLNVSGGYRLADPGADLAVAAALVSALADRPLGRDAVW
                     FGEVSLAGEVRPVAHGALRLRESAKLGFGRAYGPGDSGKGNGKGKADGALRHDAISRL
                     PILVDRIMGDA"
     gene            2280..2897
                     /locus_tag="VFF98_10095"
     CDS             2280..2897
                     /locus_tag="VFF98_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZU64023.1"
                     /translation="MSGLDILVFGVVGLAAAFGFMRGFVQETLSLAAFVVGVVAIRVL
                     HAPLTADLLGPVGSATGASVLAFLLLLVVPYAATRLLARQLGRAARDSALGPVDRVLG
                     FGFGAVKGALVVVVLFALVALAYDTVWGAAGRPAWMKHARSYAFLDASSEALVAKLAE
                     RRRAAAAANTAGDATDDAASDEAASSAPAPAPHRSAVRHHRHVAP"
     gene            2899..>3293
                     /locus_tag="VFF98_10100"
     CDS             2899..>3293
                     /locus_tag="VFF98_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZU64024.1"
                     /translation="MASVAALYTPEVLGLATSLAQWPLGTDLPLLGRARSASCGSVID
                     IGLALDGEGRIAALGLAAQACAVGQAAAALFATHATGRNAQDIAAAEAALARWLAGEA
                     PLPDWPGLAAIAAAQGYPGRHGAVLLAWRA"
BASE COUNT          455 a         1048 c         1280 g          510 t
ORIGIN      
        1 cgctcgtcgg catgatcgta gatttcgtag gcgcaggcac cgagcgcggc ggcggcggtc
       61 agccagtctg gccagcgcag gccgcctgtc aggcgccgct ccatcagcat gcccagcgca
      121 aagaaggcgc cgtggcgcag cagggccaga tcggtgtagc ggccccactg gtcacgcggg
      181 gcgctgtggc ccatccagcg atagagccag aaaccggcgc aggcgagcgt gagcgcggcc
      241 ggcagccagc gcagcggcca gcgcctgccg ccccacaggc acagcgccac cagcaggtag
      301 aaatccactt ccagccccag cgtccaccac gaaccgtcga tctggtcgag cggggagagg
      361 aagaaggcgg aagcggccca ttcatccaga atgatatgcg gcttttccag ccccgccacc
      421 agcacgcagg ccgccgacag gctggcgcac agccaggccg ccggcagcag gcgcagcgcg
      481 cggcggcgga tgaaggcgcc cggcgcgctg ccctcggccg agcgggcgat gacatagccg
      541 gagaggataa agaagatttc caccccgatc cagccgaacc agctgtagcc caccagcatg
      601 cgcggcaggg aaacgcccgt cagcgcgttg cggctgaccg ccgagggcgc ggccagatag
      661 gcagcgcccg catgataggc ccacaccagc gccgcagagc accagcgcag cagatcaagg
      721 gcggcaatgt tgcgggaggc cttggccgaa gcggcggcgg aacgggttgc gcacatggcg
      781 gggccccatg ccaaaggcgg gcggccgagg gaatagggaa aaacgccggg gggcgggggc
      841 gtggccttgc gttccccata cgttcctgct agccggaggc atggccaagc ccaaacgccg
      901 tttcgtctgc tctgcctgtg gcggggtgtc ttcccgctgg caggggcaat gcgccgattg
      961 cggcgaatgg aacacgctgg ccgaagacgc gccgcagacg gtgttttcgg tgaagcacaa
     1021 tctgtcgtcg ggcgggcggg cctttgcgtt cgagccgctg gatgcggcgg tgccgccgct
     1081 ggcgcgggcc agtacagggc tggcggaatt cgaccgggcg ctgggcggcg ggctggtgcc
     1141 gggcagcgcc attctgatgg gcggcgatcc gggcatcggc aaatccaccc tgctgctgca
     1201 ggcctcggcg gcggtggcgc aaaggggcgg ggtggcggtg tatatcagcg gcgaggaagc
     1261 gcccggccag gtgcgcctgc gggccgagcg gctggggctg gccaaggcgc cgatccggct
     1321 ggcggcggcc acttcggtgc gcgatatcct caccacgctg gggcagatgg agccgcccga
     1381 gctgctggtg atcgattcga tccagtgcat gcattccgac acgatcgagg gggcgccggg
     1441 cacggtcagc caggtgcgcg gctgcgcttt cgagctgatc cgctatgcca aggagaatgg
     1501 cgtggcgctg gtgctggtgg gccatgtgac caaggatggc agcatcgccg gcccgcgcgt
     1561 gctggagcat atggtggatg tggtgatggc cttcgagggg gagcgcagcc accagtatcg
     1621 catcctgcgc aacctgaaga accgctttgg cccggtggac gagattggcg tgtttgccat
     1681 ggaaggggcg gggctggccg aagtggcgaa cccctcgatg ctgttccttt ccggccggga
     1741 ggagcaggtg gccggcagcg cggtgttccc ggcgatggag ggcacgcggc cggtgctggt
     1801 ggagatccag gcgctgatcg tgcggctgca atcgggcgcc acgccgcgcc gggcggtggt
     1861 gggctgggac aatgggcgcc tggccatgct gctggcggtg ctggaggcgc gctgcgggct
     1921 caatttctcc tcggcggaag tctatctcaa tgtctcgggc ggctatcggc tggcggatcc
     1981 gggggcggat ctggcggtgg cggcagcgct ggtctcggcg ctggcggatc ggccgctggg
     2041 gcgcgatgcg gtgtggtttg gcgaggtgag cctggcgggc gaggtgcgcc cggtggccca
     2101 tggcgccttg cgcctgcgcg aaagcgccaa gctgggcttt ggccgcgcct atggcccggg
     2161 cgatagcggc aagggcaatg gcaagggcaa ggcggatggc gcgctgcgcc acgatgcgat
     2221 cagccgcctg ccaattctcg ttgaccggat catgggcgac gcataaccct gccggcacta
     2281 tgagtggcct tgatattctt gtgtttggcg tggtcggcct ggccgcagcc tttggtttca
     2341 tgcggggttt cgtgcaggaa accctgtcgc tggcggcctt tgtggtgggc gtggtggcga
     2401 tccgggtgct gcatgcgccg ctgacggccg acttgctggg gccggtgggt tcggccacgg
     2461 gcgcttcggt gctggcgttc ctgctgctgc tggtggtgcc ctatgccgcc acgcgcctgc
     2521 tggcccgcca gctgggccgg gccgcgcgcg attcggcgct gggcccggtg gacagggtgc
     2581 tgggctttgg ctttggcgcg gtgaagggcg cgctggtggt ggtggtgctc ttcgcgctgg
     2641 tggcgctggc ctatgacacg gtgtggggcg cggcgggccg gccggcgtgg atgaagcatg
     2701 cccgttccta tgcctttctc gatgccagca gcgaggcgct ggtggccaag ctggccgagc
     2761 gccgccgcgc ggccgctgcc gccaacactg ccggtgatgc cacggatgat gccgcctccg
     2821 atgaggccgc cagcagcgcc ccggcccccg cgccgcatcg ctccgctgtc cgccatcatc
     2881 gccacgtggc gccatagcat ggcctctgtc gccgcgcttt acacgcccga ggtgctgggg
     2941 ctggccacct cgctggcgca atggccgctg gggacggatc tgccgctgtt ggggcgggcg
     3001 cgctcggcca gctgcggcag tgtgatcgat atcggcctgg cgctggatgg cgagggccgc
     3061 atcgccgcgc tggggctggc agctcaggcc tgcgccgtgg ggcaggcggc cgcggcgctg
     3121 ttcgccaccc atgccaccgg ccgcaacgcc caagacattg ccgccgccga ggctgctctg
     3181 gcccgctggc tggccggtga ggcgcccctg cccgattggc ccggccttgc cgcgattgcc
     3241 gccgcgcaag gctatcccgg ccgccatggc gcggtgttgc tggcatggcg ggc
//
LOCUS       DASRYE010000193        14302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_26445,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000193 DASRYE010000000
VERSION     DASRYE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14302
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26445"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFF98_10105"
     CDS             <1..829
                     /locus_tag="VFF98_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446229.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HZU64025.1"
                     /translation="SFSFEMESSAALGFGFRCGFLGLLHLEIIQERLSREYDLDLITT
                     APSVVYRITMKDGTVKEMHNPSDMPDPVKIETMEEPWIRATIYTPDEYLGSILKLCQD
                     RRGIQTGLTYVGGRAQVNYELPLNEVVFDFYDRLKSISRGYASFDYEQIGLREGDLVM
                     MNILVNNEPVDALSMIVHRSQADPRGRQLCERLKDLIPRHLFKIPIQAAIGAKVIARE
                     TIAAMRKDVTAKCYGGDITRKKKLLEKQKEGKKRMREYGSVQIPQEAFIAALRMGEE"
     gene            complement(947..1639)
                     /locus_tag="VFF98_10110"
     CDS             complement(947..1639)
                     /locus_tag="VFF98_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZU64026.1"
                     /translation="MIIALAVSASAFAATPAAAETANWAGPYVGAVVGVTNTHSNVND
                     NDSEISSWGNSGALNGQRTSVTGGLTGGYNFQSGPFVYGVEGDVSLTDANTKANGYSV
                     NTYVHTRQHWSATARARAGIAIGNVLPYFTAGLSIGETRHEVYDYGAQDGYTGCGASG
                     SDTGWVCQTKTGVGYAVGGGVEGKVSKHVSVKAEYLHTTLPTATAYLYSYSPIHFSDS
                     YDTFRVGVNYHF"
     gene            complement(1953..3167)
                     /gene="hemA"
                     /locus_tag="VFF98_10115"
     CDS             complement(1953..3167)
                     /gene="hemA"
                     /locus_tag="VFF98_10115"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HZU64027.1"
                     /translation="MNYDQIFDAAIDRLHSEGRYRVFIDILRNKGAYPNARCFHGHNG
                     PKPITVWCSNDYLAMGQHPKVIAAMEEALHDVGAGSGGTRNIGGNTHYHIDLERELAD
                     LHGKEGALLFTSGYVSNDATLSTLAKILPGCIIFSDELNHASMIAGIRNSGAEKRVFR
                     HNDLEHLEELLAEVDPATPKLIAFESVYSMDADIAPIHAICDLADKYNALTYIDEVHA
                     VGMYGPRGGGITDRDDAAHRIDIIEGTLGKAFGVMGGYIAASTKVIDVIRSYAPGFIF
                     TTSLSPVLVAGVLASVRHLKSSSVERDAQQAAAAMLKQLFRDAGLPVMDSTTHIVPLM
                     VGDPVKAKAISDILLAEYGVYVQPINFPTVPRGKERLRFTPGPAHTEAMMRELTAALV
                     EIWDRLELKLAA"
     gene            complement(3322..4137)
                     /gene="murI"
                     /locus_tag="VFF98_10120"
     CDS             complement(3322..4137)
                     /gene="murI"
                     /locus_tag="VFF98_10120"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZU64028.1"
                     /translation="MSLSSPVAVDPAAPILLFDSGVGGLSVLEALRRQLPDAPVIYAA
                     DTAGLPYGEKSEAQIAARVSGLLGRMTERLKPRLVCIACNTASTIALSAVREVLEVPI
                     VGTVPAIKPAAERTCTGVIGLLGTAATIRQAYVDRLEAEYAHGHRLLRFGAGELVAAA
                     EAKLRGQRPDPAAIAHAVAGLRSQPDGARIDTVVLACTHFPLLAEELGAAFGPDVALV
                     DGAAGIARRIAFLTQGQAFARARPDYALFTSDAGIAALAPALQKRGLAEWAVF"
     gene            4296..4886
                     /gene="plsY"
                     /locus_tag="VFF98_10125"
     CDS             4296..4886
                     /gene="plsY"
                     /locus_tag="VFF98_10125"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZU64029.1"
                     /translation="MEAGVLAGLAGGYALGSVPFGLLLTRAAGAGDIRAIGSGNIGAT
                     NVLRTGRKGLAAATLLLDMLKGLAAVLLARALWGEQAGLAAAAGAMVGHCFPVWLRFR
                     GGKGVATMLGVSAGLGWAIGLVAALAWLGCFRLSRISSVGGMGAALAAPIAAVALGEW
                     GKAPLLAALALLVVAKHHANIARLIAGEEPRVGRKG"
     gene            4889..6067
                     /gene="dprA"
                     /locus_tag="VFF98_10130"
     CDS             4889..6067
                     /gene="dprA"
                     /locus_tag="VFF98_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZU64030.1"
                     /translation="MAAVPPGRAAPLSHAEGLARIRLLRSARIGPVTFHQLLRQFGTA
                     EAALAALPELAARGGLGRAGAQNYRPAAQGAIEAEIAATRRAGGRYLFHDSPDYPPLL
                     RLADGAPPVLVARGDVRRAQGAAVALVGARDASGAARFLAREWGQKLAEMGFPVVSGL
                     ARGVDAAAHEGALAAATGAGTVAVVAGGVDVAYPPEHADLQARIAAEGLLLSEAPCGT
                     QPTNRHFPARNRIIAGLAAGTVVVEAALRSGSLITARMAAEYGREVMAVPGSPLDARS
                     HGTNTMIREGAILVQRVDDIAELLSHFDGQVHSRFAPERLRAAAAEAELPLAPAQAEA
                     GADLAGRIAALLSPAPLPVDELVRQSGEAAASVQMALIELELAGRLQRHAGGKVSCQP
                     "
     gene            6064..7056
                     /locus_tag="VFF98_10135"
     CDS             6064..7056
                     /locus_tag="VFF98_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64031.1"
                     /translation="MTAADLPPPDYIRQIRDQITGQRWMKLDGRLPARLPHVPEAEAA
                     LAQMDLHEVIAIYINWLLRHLPQEKRHLHLSDRIAPALAGLSMRQRAGYELVCERLAS
                     GGDLEPYLSRRVDRLWRPPLPERAGKPHPVLPLDGLLNDWGLHHLHLIAAHGRHRRHD
                     AEDTPVLILAVRAGAAWLVDIVEHGIGHAAVWTDIALVETVLRNWPEAGLVHVLKQTD
                     GCHWPELSAEEMRRLRDAGIPYELCVDGLVIQPEGMSASGVSGRLAHAVTALTRALRQ
                     LQAEAEAGEGPLVHWLGHVGLPGRPAEWQVAINASALVVWRPGSPRVTLMRWRP"
     gene            7229..9817
                     /gene="topA"
                     /locus_tag="VFF98_10140"
     CDS             7229..9817
                     /gene="topA"
                     /locus_tag="VFF98_10140"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZU64032.1"
                     /translation="MQLVIVESPAKAKTIEKYLGKDYRVLASYGHVRDLPAKDGSVRP
                     DEGFAMDWELYGDKQARVREIAELAKEADRLILATDPDREGEAISWHVRELLAKRRAL
                     PKAVERVTFNAITKDAVTTAMKHPRELDQDLIDAYLARRALDYLFGFTLSPVLWRKLP
                     GAKSAGRVQSVALRLIVQREREIELFRPQEYWQVVARLMQDGTEFSARLVQFEGQKLE
                     KLSLGEEGIALRAKAAVEAGAFRVEEVETKPVRRNPQPPFTTSTLQQEAARKLGFSAS
                     HTMRVAQHLYEAGAITYMRTDGVQMDPSAISAARAEITERFSGHFCPEKPRHYETKAK
                     NAQEAHEAIRPTDFGRDSYGSGDDARLYELIWKRALASQMASAALERTSITLRDGTGQ
                     HALRATGQVVKFPGFLAVYEEGRDQKGEDDDDSGLLPAMASGDMPAKQGVEASQHFTQ
                     PPPRYSEASLVKRLEELGIGRPSTYAATLQVLKDRAYVRTEKNRFFAEETGRLLTAFL
                     ERFFPRYVAYEFTAGMEEELDDVSGGRAEWRAVLEAFWRDFKPKSDEVMEKKPSEVTE
                     ALDEFLSDYLFPPKEDGSDPRACPQCDGGRLALRGGRYGAFIACSNYPECKFTRKFAQ
                     AGGSGDAGQEGELGKHPETGEPILRKAGRFGPYIQMGDGEGKKMASIPKDIGELTVDW
                     AAKLLSLPRTIGQHPETGEPITASIGRYGPYLAHKGKYARLKSTAEVFDTGMNMAVAR
                     LAEAANGGGRGQRTPAEPLKTFGAHPTSGGEIKLLAGRYGPYVTDGTTNATLPKDKDP
                     AALSAEEAIALIDERAAKAPAKGKKKAPAKKAPPKKPAAKKADGEAKPAAKKAPAKRK
                     VAAAGE"
     gene            complement(9861..11384)
                     /locus_tag="VFF98_10145"
     CDS             complement(9861..11384)
                     /locus_tag="VFF98_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZU64033.1"
                     /translation="MESAASQRRTAAAPRVWPSEMTRAGLAYLVLALLLRGATLGYPM
                     IHIDEQFYLLVGDRMLHGQLPYIDIWDRKPIGLFLLYGAIRLLGGEGIVQYQLVALAF
                     TVATSLAIFRMARMIAPSSGAFWAGVGYQLYLSAYFCFGGQAPVFYNLLVALSALGMV
                     NILGSASARRLVWGGLGLMLVLGLAIQIKYTVIFEGAAFGMALMAHGRAMGWSRLKVF
                     CTGVLWAFTALLPGLVALAYYAAIGHGADYVQANFLSIFDRHEDFLGSLWRLTKEMLA
                     LIPVWIALFWAPGRFPPVTGNNPLAIGFLRYWGLAAFLGFLVFGTWYDHYVAPLLVPL
                     LVLAAPALGRARPYLWPTVLLAVIGTLAAAIVTTYNVNHHGTRAQLDHLADLIRAERG
                     DGCAYINEGDPVLYLKSGACFTTRFVFPNHLNGMVDAKALGVNVDDEIARIMSTHPQV
                     VVMSVYPSSLPVNWPARYAIFQALRRDYKVVAYTSVGWRDFLVYRYKGVQPAIAQIH"
     gene            complement(11587..12528)
                     /gene="era"
                     /locus_tag="VFF98_10150"
     CDS             complement(11587..12528)
                     /gene="era"
                     /locus_tag="VFF98_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZU64034.1"
                     /translation="MTDISLPPPPSGLPEHCGLVAVIGAPNAGKSTLVNALVGQKVAI
                     VSPKAQTTRARLLGIALEQSESANAQIILADTPGLFAPRRRLDRAMVNAAWEGAEEAD
                     AILLVVDAAKKNREYLPPILETLAHRRERKLLVLNKVDLTAKEPLLALAQELTQAVPF
                     DEVFFISASTQDGVAQLKTRLAALMPQGPWHYPEDQVSDASERLMACEITREQLYRQL
                     HDELPYDSTVRPESYRTRKDGSVEIHQQIVIARDSQKPIVLGKRGARLKAIGEAARTE
                     LASLLGVKVHLFLHVKVDEAWADSREIYEEIGLDWVR"
     gene            complement(12525..13142)
                     /gene="rnc"
                     /locus_tag="VFF98_10155"
     CDS             complement(12525..13142)
                     /gene="rnc"
                     /locus_tag="VFF98_10155"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZU64035.1"
                     /translation="MPASPHWLSALTHGSLGETRDYQRLEFLGDRVLGLVIADWLFET
                     EGEAEGRLSQRLNVLVSRATCAGVARQIGLGPHIRLGKQARDDGGADSDNILGDVMEA
                     LIGASFLTLGFEPTRAIVRRLWADVFGGKAGQSKHPKSALQEWAAGNRRAMPEYRLVD
                     RSGPDHAARFTVAVAVRNVGETQATGASKQEAETLAAVEFLRRFG"
     gene            complement(13220..14122)
                     /gene="lepB"
                     /locus_tag="VFF98_10160"
     CDS             complement(13220..14122)
                     /gene="lepB"
                     /locus_tag="VFF98_10160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZU64036.1"
                     /translation="MTEPATPPPADSPARAEGPAAPAAPARKEKEESFLVFLLKLVVL
                     VAVFRSFIFSPFNIPSESMLPRLVNGDYLLAAKWPYGFSSYSLPFSVPLLPGRMLAHQ
                     PVRGDVVIFKAPPANEQDWIKRVIGLPGDIVQMQNGVLYLNGKAIPKVKIEDFEIAVS
                     ANTSCYQPHYTQGLNYEVVKPDGSPNGRHVCRYPQYRETLPAEDGRPAKSFNVLDLEQ
                     TPQDNTPPYLVPEGSLFLMGDDRDNSMDSRFSTIEGGIGMVPQANLVGRATIVMWSTD
                     GGAQWYAPWTWFTSARWRRIGGLF"
BASE COUNT         2456 a         4618 c         4981 g         2247 t
ORIGIN      
        1 gtccttctcg tttgaaatgg agagcagcgc ggcgctgggc ttcggcttcc gctgcggctt
       61 ccttggcctc ctccatctgg aaatcatcca ggagcggctg agccgcgaat atgatctgga
      121 tctcatcacc acggcgcctt cggtggtcta tcgcatcacc atgaaggacg gcacggtgaa
      181 ggagatgcac aacccttccg acatgcccga tccggtgaag atcgagacga tggaggagcc
      241 gtggatccgc gccaccatct acacgccgga cgagtatctg ggcagcatcc tgaagctgtg
      301 tcaggaccgc cgcggtatcc agacgggcct cacctatgtc ggcgggcgcg ctcaggtgaa
      361 ttacgagctg ccgctcaacg aggtggtatt cgacttctac gaccggttga agtcaatctc
      421 gcgcggctat gccagcttcg attacgaaca gatcggcctg cgcgagggtg atctggtgat
      481 gatgaacatc ctcgtcaaca acgagccggt cgatgcgctc tcgatgatcg tccaccgcag
      541 ccaggccgac ccgcgcggcc gccagctgtg cgagcggctg aaggacctga tcccgcgcca
      601 cctgttcaag atccccattc aggccgccat cggcgccaag gtgatcgcac gcgagaccat
      661 cgctgccatg cgcaaggacg tgaccgccaa atgctatggc ggcgacatta cccgcaagaa
      721 gaagctgctg gaaaagcaga aagagggcaa gaagcgcatg cgcgaatatg gcagcgtgca
      781 gatcccgcag gaggccttca tcgccgccct tcgcatgggc gaggaatgag cggcgaggaa
      841 taagagggcc cttttccggc atacgcgaaa agggggcggg aagaaccatc ttcccgcccc
      901 ctttttctgt gtggcggggc ctgtcctgca gcaggcccca accaactcag aagtggtagt
      961 tcacgccaac gcggaacgtg tcatagctgt ccgagaagtg gatcgggcta tagctgtaga
     1021 ggtaggcggt ggccgtcggc agggtggtgt ggaggtattc cgccttcacc gacacatgct
     1081 tcgagacctt gccttccacg ccgccgccca cggcatagcc aacgccggtc ttggtctggc
     1141 agacccagcc ggtgtcgctg cccgaggcgc cgcagccggt gtagccgtcc tgcgcgccgt
     1201 agtcgtagac ctcgtggcgg gtttcgccga tgctgaggcc ggcggtgaag tagggcagca
     1261 cgttgccgat ggcgataccg gcgcgggcgc gggcggtggc gctccagtgc tggcgggtgt
     1321 gcacataggt gttgacgcta tagccgttgg ccttggtgtt ggcgtcggtc aggctgacat
     1381 cgccttccac gccatagacg aacgggccgc tctggaagtt gtagccgccg gtgaggccgc
     1441 cggtcacgct ggtgcgctgg ccgttgaggg cgcccgaatt gccccacgac gagatctcgc
     1501 tgtcgttgtc gttgacgttc gagtgggtgt tggtcacgcc gaccacggcg cccacatagg
     1561 ggccggccca gttggcggtt tcggcggcgg cgggggtggc tgcgaacgcg ctggcggaca
     1621 ccgccagagc aatgatcatg ttcctcattt tcttaccttg tctattgggg gtattaggtg
     1681 cgcacccgag gcgcaattga tccccacagg cggcgaagac aagtgcaaaa atggcaaata
     1741 atcttgcgat cataatggct tgatagagtt tgacagtttt ttgacacgca tgttagtagc
     1801 cgccggtttg gcgcctgaaa cgaggcgttt ttctccattg caccgggcca gcctgcacac
     1861 gcattgcgcc ccggaaaacg gcggcaaaac cgtttcccgg ggcgcaatat acgtgcaggc
     1921 ccagcgcttg ccgggcgggg atgagggggg cgtcaggccg ccagtttcag ctccagccgg
     1981 tcccagattt ccaccagggc ggcggtcagc tcgcgcatca tcgcctcggt gtgggccgga
     2041 cccggggtga agcgcaggcg ctccttgccg cgcggcacgg tggggaagtt gatcggctgc
     2101 acatagacgc catattcggc cagcagaatg tcgctgatcg ccttggcctt caccggatcg
     2161 cccaccatca gcggcacgat atgggtggtg ctgtccatca ccggcaggcc ggcatcgcgg
     2221 aacagctgct tcagcatggc agcggcggcc tgctgggcat cgcgctccac gctgctgctc
     2281 ttgaggtggc gcaccgaggc cagcacgccg gccaccagca ccggcgaaag cgaagtggtg
     2341 aagatgaagc ccggcgcata ggagcggatc acgtcgatca ccttggtgct ggcggcgata
     2401 tagccaccca tcacgccaaa ggccttgccc agcgtgccct cgatgatgtc gatccggtgg
     2461 gcggcatcgt cgcggtcggt gatgccgccg ccgcgcgggc catacatgcc caccgcgtgc
     2521 acctcgtcga tataggtgag cgcgttgtac ttgtcggcga ggtcgcagat ggcgtggatg
     2581 ggggcgatat cggcatccat cgaatagacc gattcgaaag cgatcagctt gggcgtggcc
     2641 gggtccacct cggccagcag ctcttccaga tgttccagat cattgtggcg gaacacgcgc
     2701 ttttccgcgc ccgaattgcg gatgccggcg atcatgctgg catggttcag ctcgtcggaa
     2761 aagatgatgc agccgggcag gatcttggcc agcgtggaca gcgtcgcatc gttcgagaca
     2821 tagcccgagg tgaacagcag cgccccttcc ttgccgtgca ggtctgccag ttcgcgttcg
     2881 agatcaatgt ggtaatgggt gttgccgccg atgttgcggg tgccgccaga gccggcgccg
     2941 acatcgtgca gcgcttcctc catggcggcg atcaccttgg ggtgctggcc catcgccaga
     3001 taatcgttgc tgcaccacac ggtgatcggc ttggggccgt tgtggccgtg gaagcagcgc
     3061 gcgttgggat aggcgccctt gttgcgcaga atgtcgataa agacacgata gcgcccctcg
     3121 gaatggagcc tgtcgatcgc ggcgtcgaaa atctggtcgt agttcacgtg gcttttgtcc
     3181 tgctcggcgg ccgtttagcc cggtgcctgc ctccccgata gggcatatcg tcccgcgccg
     3241 aaaatgacca gcatcaaaca gcccgctagg acaaactgta ccaattgccg agaagggcgg
     3301 gggcagtttg gccggaaagg atcaaaaaac cgcccactcg gccaatccgc gtttttgcag
     3361 ggcgggggca agggcggcga tgccggcatc gctggtgaac agggcataat cgggccgggc
     3421 gcgggcaaag gcctgcccct gcgtgaggaa ggcgatgcgc cgggcaatgc cggccgcgcc
     3481 atccaccagc gccacatcgg ggccgaaggc ggcgcccagt tcctctgcca gcagggggaa
     3541 atgggtgcag gccagcacca ccgtgtcgat gcgcgcgcca tcaggctggc tgcgcagccc
     3601 cgccacggca tgggcaatgg cggcaggatc gggccgctgg ccgcgcagct tggcttcggc
     3661 ggcggccacc agttcgcccg cgccaaagcg cagcaggcgg tggccatggg catattcggc
     3721 ctccagccgg tccacatagg cctgccggat ggtggccgcg gtgcccagca ggccgatcac
     3781 gccggtgcag gtgcgctctg ccgccggctt gatcgccggc acggtgccga cgatcggcac
     3841 ttccagcacc tcgcgcacag cggaaagggc aatggtgctg gccgtgttgc aggcaatgca
     3901 cactaggcgc ggcttcagcc gctccgtcat ccggcccagc aggcccgaga cgcgcgcggc
     3961 aatctgcgcc tcgctcttct cgccataggg caagccggcg gtatcggcgg catagatcac
     4021 cggtgcatcc ggcagctgac ggcgcagcgc ctccagcacg gacaggccgc cgacgccgga
     4081 atcgaacagc aggatcggcg cggcaggatc gacggcaaca ggagaggaga gggacaagcg
     4141 ctggcctttg cgggaatcgg gaggggaggg cgcggtcttt ctctatcgcc cggcggggca
     4201 aaaagaaaag agagggcttc gccattggcc cacagccggc taagggctat ggccgaaaga
     4261 gggccgcagg gcccatcgcg gtgaggacaa aagcaatgga agcaggcgtt ttggcaggtt
     4321 tggctggggg ctatgccctg ggctcggtgc cgttcggcct gttgctgacg cgggccgccg
     4381 gcgcgggcga cattcgcgct atcggttctg gcaatatcgg cgccaccaat gtgctgcgca
     4441 ccggccgcaa ggggctggcg gcggccacct tgctgctgga catgctgaag gggctggcag
     4501 ccgtgctgct ggcccgcgcg ctgtggggcg agcaggcggg gctggcggcg gcggcgggcg
     4561 ccatggtggg ccattgcttc ccggtgtggc tgcgttttcg cggcggcaag ggggtggcca
     4621 ccatgctggg cgtttcggcc gggctgggct gggcgatcgg gctggtggca gcgcttgcct
     4681 ggctgggctg ctttcgcctg tcgcgcattt cctcggtggg gggcatgggg gcggcccttg
     4741 ccgcgccgat cgccgctgtg gcgctggggg aatggggcaa ggcgcccttg ctggcggcgc
     4801 tggcgctgct ggtggtggcc aagcatcatg ccaatatcgc ccggctgatc gccggcgagg
     4861 agccacgggt gggccgcaag ggctgaccat ggccgccgtg ccgccaggcc gggccgcgcc
     4921 gctctcccat gcggaagggc tggcgcgcat ccgcctgctg cgctcggcgc ggatcgggcc
     4981 ggtcactttc caccagttgc tgcgccagtt cggcactgcc gaggccgcgc tggcggccct
     5041 gccggaactg gccgcgcgcg gcggcctggg gcgggcgggg gcgcagaact atcgcccggc
     5101 cgcgcagggg gcgatagagg ccgaaattgc cgccacccgg cgcgcgggcg ggcgctatct
     5161 cttccatgat tcgcccgatt atccgccgct gctgcgcctg gccgatggcg cgcccccggt
     5221 gctggtcgcc cggggcgatg tgcggcgcgc gcaaggggcg gcagtggcgc tagtgggcgc
     5281 gcgcgatgcc tcgggcgcgg cgcgcttcct ggcgcgcgaa tgggggcaga agctggcgga
     5341 gatgggcttt ccggtcgtct cggggttggc gcggggggtg gatgcggcgg cccatgaagg
     5401 cgcactggct gcggccacag gcgccggcac ggtggcggtg gtggccggcg gcgtggatgt
     5461 ggcctatccg cccgaacatg ccgatctgca ggcgcgcatc gctgccgagg gcctgctgct
     5521 gagcgaagcg ccctgcggca cccagcccac caaccgccat ttccccgcgc gcaaccgcat
     5581 catcgccggg ctggcggcgg gcacggtggt ggtggaagcg gcgctgcgtt ccggcagcct
     5641 gatcaccgcg cgcatggcgg ccgaatatgg ccgcgaggtg atggccgtgc cgggatcgcc
     5701 gctggatgcg cgcagccatg gcaccaacac gatgatccgc gagggcgcca ttctggtgca
     5761 gcgggtggac gatatcgccg agctgctcag ccatttcgac gggcaggtgc acagccgctt
     5821 cgcccccgaa cgcctgcgcg cggccgccgc cgaggccgag ctgccgctgg ccccagccca
     5881 agccgaagcg ggcgccgatc tcgccgggcg catcgccgcg ctgctttcgc ccgcgcccct
     5941 gccggtggat gagctggtgc gccagagcgg cgaggcggcg gccagcgtgc agatggcgct
     6001 gatcgagctg gagctggccg ggcggctgca gcgccacgcc ggcggcaagg tcagctgcca
     6061 gccatgaccg cggccgatct gcccccgccc gactatatcc gccagatccg cgaccaaatc
     6121 accggccagc gctggatgaa gctggatggc cgcctgcccg cccgcctgcc ccatgtgccc
     6181 gaggccgagg cggcgctggc gcagatggac cttcacgagg tgatcgccat ctacatcaac
     6241 tggctgctgc gccacctgcc gcaggaaaag cgccatctcc acctttccga tcgcattgcc
     6301 ccggcgctgg ccgggctgag catgcgccag cgcgccggtt atgagctggt gtgcgagcgg
     6361 cttgccagtg gcggcgatct ggagccctat ctcagccgcc gggtggaccg gctgtggcgc
     6421 ccgccgctgc ccgagcgggc cggcaagccc cacccggtgc tgccgctcga tggcctgctg
     6481 aacgactggg ggctgcatca cctccacctc atcgccgccc atggccgcca tcgccgccat
     6541 gatgcggagg atacgccggt gctgatcctg gcggtgcggg caggggcggc ctggctggtg
     6601 gatatcgttg aacatggcat cggccatgcg gcggtgtgga cggatatcgc gctggtggaa
     6661 acggtgctgc gcaactggcc cgaggcgggg ctggtgcatg tgctgaagca gaccgatggc
     6721 tgccactggc cggaactgtc ggccgaggag atgcgccgcc tgcgcgatgc cggcatcccc
     6781 tacgagctgt gcgtcgacgg gctggtgatc cagccggagg ggatgagcgc cagtggcgtt
     6841 tccggccggc ttgcccacgc cgttaccgcg ctgacccgcg ccctgcgcca gctgcaggcg
     6901 gaggccgagg caggcgaggg gccgctggtg cattggctgg gccatgtggg cctgccgggg
     6961 cggccggccg aatggcaggt ggcgatcaat gccagcgcgc tggtggtgtg gcggccgggc
     7021 agcccgcgcg ttacgctgat gcgctggcgg ccctagaagg cagcagtagt ggctttgggg
     7081 gtggtaaaga ccccgcgccc tcttgacggc ccgcgccaaa caggggagtg gcgcaggcca
     7141 tgccgccgct gccgatcagg ccttgacgcg gacaagcctt tctcaccacc ctcgcgcgta
     7201 cgtgtgaggc aacagaaaaa ccggggctat gcaactcgtt atcgtcgaat cccccgccaa
     7261 ggcgaaaacc atcgagaaat atctcggcaa ggactatcgc gtgctggcct cttatggcca
     7321 tgtgcgcgat ctgccggcca aggatggctc tgtgcggccc gacgagggct ttgcgatgga
     7381 ttgggagctc tatggcgaca agcaggcgcg cgtgcgcgag atcgccgagc tggccaagga
     7441 agctgatcgc ctgatcctgg cgactgaccc tgaccgcgag ggtgaggcga tcagctggca
     7501 tgtgcgcgag ctgctggcca agcgccgggc gctgcccaag gcggtggaac gggtgacctt
     7561 caacgccatc accaaggatg cggtgaccac ggcgatgaag cacccgcgcg agctggatca
     7621 ggatcttatc gatgcctatc tggcgcgccg cgcgctggat tatctgttcg gcttcacgct
     7681 ctcgccggtg ttgtggcgca agctgccggg ggccaaatcg gccggccgcg tgcaatcggt
     7741 ggcgctgcgc ctgatcgtcc agcgcgagcg cgagatcgag ctgttccgcc cgcaggaata
     7801 ttggcaggta gtggcccggc tgatgcagga tggcacggaa ttttccgccc gcctggtgca
     7861 gttcgagggg cagaagctgg agaagctgtc gctgggcgag gaaggcattg ccctgcgcgc
     7921 caaggcggcg gtggaagccg gcgccttccg cgtggaagag gtggagacca agcctgtccg
     7981 ccgcaatccg cagccgccct tcaccaccag cacgctgcag caggaagcgg cccgcaaact
     8041 gggcttttcg gccagccaca cgatgcgcgt ggcccagcat ctctacgagg cgggcgccat
     8101 tacctatatg cgtaccgatg gcgtgcagat ggacccttcg gccatctctg ccgcccgggc
     8161 cgagatcacc gagcggtttt cgggccattt ctgcccggaa aagccccgcc actacgagac
     8221 caaggccaag aacgcgcagg aagcccacga ggcgatccgc cccaccgatt tcgggcgtga
     8281 cagctacggc agcggcgatg acgcgcgcct ctatgagctg atctggaagc gggcgctggc
     8341 cagccagatg gcctcggccg cgctggagcg caccagcatc accctgcgcg atggcaccgg
     8401 ccagcacgcc ctgcgcgcca ccggccaggt ggtgaaattc cccggcttcc tcgccgttta
     8461 cgaggaaggg cgcgaccaga agggcgagga cgacgacgac agcggcctgc tgcccgccat
     8521 ggcgagcggt gacatgcccg ccaagcaggg cgtggaagcc agccagcatt tcacccagcc
     8581 gccgccgcgc tattcggaag ccagcctcgt caagcggctg gaggagctgg gcattggtcg
     8641 gccctccacc tatgccgcca cgctgcaggt gctgaaggac cgcgcctatg tgcgcaccga
     8701 gaagaaccgc ttcttcgccg aggaaaccgg ccgcctgctg accgccttcc tcgaacgctt
     8761 cttcccgcgc tatgtggcct atgagttcac tgccggcatg gaagaggagc tcgacgatgt
     8821 ttcgggcggg cgggccgaat ggcgcgcggt gctggaagcg ttctggcgcg atttcaagcc
     8881 gaagtctgac gaggtgatgg agaagaagcc ctcggaagtg accgaggcgc ttgatgaatt
     8941 cctctcggac tatctgttcc cgccgaagga ggatggcagc gatccgcggg cctgcccgca
     9001 atgcgacggc gggcgcctgg cgctgcgcgg cgggcgctat ggcgcgttca tcgcctgctc
     9061 caactacccg gaatgcaagt tcacccgcaa gtttgcccag gccggcggca gcggtgatgc
     9121 cggccaggaa ggcgagctgg gcaagcatcc ggaaaccggc gagccgatcc tgcgcaaggc
     9181 cggccgcttt ggcccctata tccagatggg cgatggcgag ggcaagaaga tggcctccat
     9241 ccccaaggac atcggcgagc tgacggtgga ctgggcggcc aagctgctga gcctgccgcg
     9301 caccatcggc cagcatccgg aaacgggtga gccgatcacc gccagcatcg gccgctatgg
     9361 cccctatctg gcgcacaagg gcaaatatgc ccggctgaag agcaccgccg aagtgttcga
     9421 caccggcatg aacatggccg tggcgcgcct ggccgaagcg gccaatggcg gcggccgcgg
     9481 ccagcgcacg ccggcagagc cgctgaagac ctttggcgcc catcccacca gcggcggcga
     9541 gatcaagctg ctggcggggc gctatggccc ctatgtgacc gatggcacca ccaacgccac
     9601 cctgcccaag gacaaggacc ccgcagcgct gagcgccgag gaagccatcg cgctgatcga
     9661 cgagcgggcc gccaaggctc cggccaaggg caagaagaag gcgccggcca agaaggctcc
     9721 gcccaagaag cctgcggcca agaaggccga tggcgaggcc aagccggcgg ccaaaaaggc
     9781 gccggccaag cgcaaggtgg ccgccgccgg ggaatgatga aaaaggcgcg gggggcttgc
     9841 ctgccctccg cgccttttct tcaatggatc tgggcgatgg ccggctgcac gcccttgtag
     9901 cgatagacga ggaaatcgcg ccagcccacg ctggtatagg ccaccacctt gtagtcgcgg
     9961 cgcagggcct ggaagatggc atagcgcgcc ggccagttca ccggcagcga cgagggatag
    10021 acgctcatca ccaccacctg cgggtgggtg gacatgatgc gggcgatctc gtcatccaca
    10081 ttcacgccca gggccttggc atccaccatg ccgttcagat ggttggggaa gacaaagcgc
    10141 gtggtgaagc aggcgcccga cttcagatac agcaccggat cgccctcgtt gatataggcg
    10201 cagccatcgc cgcgttcggc gcggatgaga tcggccagat gatcgagctg ggcgcgggtg
    10261 ccgtggtggt tcacattgta ggtggtgacg atcgcggcgg ccagcgtgcc gatcaccgcc
    10321 agcagcacgg tgggccacag atagggccgg gcccggccca gcgcgggcgc tgccagcacc
    10381 agcaacggca ccagcagcgg cgccacgtaa tgatcatacc acgtgccgaa gacgaggaag
    10441 ccaaggaagg ctgccagccc ccaatagcgc aggaagccga tcgccagcgg attgttgccg
    10501 gtgaccggcg gaaaccggcc cggcgcccag aacagggcga tccacaccgg gatcagcgcc
    10561 agcatttcct tcgtcagccg ccacagcgag ccgaggaaat cctcgtgccg gtcgaagatc
    10621 gacaggaaat tggcctgcac ataatcggcc ccgtggccga tcgcggcata ataggccagc
    10681 gccacaaggc ccggcagcag cgccgtgaag gcccagagca cgcctgtgca gaacaccttc
    10741 agccggctcc agcccatcgc ccggccatgc gccatcagcg ccatgccaaa ggccgcgccc
    10801 tcgaagatca ccgtatattt gatctggatg gcaaggccca gcaccagcat cagccccagc
    10861 ccgccccaca ccagccgccg cgccgacgca gagcccagga tgttcaccat gcccagcgcc
    10921 gaaagcgcca ccagcaggtt gtagaacacc ggcgcctggc cgccgaagca gaaataggcg
    10981 gagagataga gctgatagcc aacgcctgcc cagaacgcgc cggaggaggg cgcgatcatc
    11041 cgcgccatgc ggaagatcgc cagcgaggtg gccacggtga aggccagcgc caccagctga
    11101 tattgcacga tgccctcgcc gcccagcagg cggatggcgc catagagcag gaacaggccg
    11161 atgggttttc tgtcccagat atcgatatag ggcagctggc catgcagcat ccgatcgccc
    11221 acgagcagat agaactgctc gtcgatatgg atcatcggat agcccagcgt ggcgccgcgc
    11281 agcagcagcg cgagaaccag ataggccaac ccggcgcggg tcatctccga tggccacacg
    11341 cggggagcag ctgcggtgcg gcgctggctt gcggcgcttt ccatccccag aaccctgtgg
    11401 ggcaaggccg cgatgaattc gaccatttat gcgatctccg aaagcacatg tcgcccggca
    11461 ggccccccca ggccaaccaa cgcgcgccgc gtgcccccac gcatgcggca agagccgcgg
    11521 ttagacaagg ttaattgccg gtgcatttcg cccttgtcgc ggaaagattg caggcaggcg
    11581 cgcaggtcag cgcacccagt caagcccgat ctcctcgtag atctcgcggc tgtcggccca
    11641 ggcctcgtcc accttcacat ggaggaagag atgcaccttc acgcccagca ggctggccag
    11701 ctcggtgcgg gccgcctcgc cgatcgcctt gaggcgggcg ccgcgcttgc ccagcacgat
    11761 gggcttctgg ctgtcacgcg cgatgacgat ctgctggtgg atctccaccg agccatcctt
    11821 gcgggtgcga tagctttcgg gccgcacggt gctgtcgtag ggcagttcgt cgtgcagctg
    11881 gcgatagagc tgctcgcggg tgatctcgca ggccatcagc cgctccgagg catccgacac
    11941 ctgatcttcc ggatagtgcc aggggccctg cggcatcagc gcagcaaggc gcgtcttcag
    12001 ctgggccacg ccatcctgcg tgctggccga gatgaagaac acctcgtcga acggcaccgc
    12061 ctgggtcagc tcctgggcca gcgccagcag cggttccttg gcggtcaggt ccaccttgtt
    12121 cagcaccagc agcttgcgct cgcgccggtg agccagcgtt tcgaggatgg gcggcagata
    12181 ctcgcggttc ttcttggcgg catccaccac cagcaggatc gcatcggctt cctcggcgcc
    12241 ttcccaggcg gcgttgacca tcgcccggtc gagccggcgg cgcggcgcaa agaggccggg
    12301 cgtgtcggcc aggatgatct gggcgttggc gctctcgctc tgttccagcg cgatgccgag
    12361 gaggcgcgcg cgggtggtct gcgccttggg cgagacaatg gcgaccttct ggccgaccag
    12421 cgcgttcacc agcgtggact tgcccgcatt gggcgcgccg atcacggcca ccaggccgca
    12481 gtgctcgggc aggcccgaag gagggggggg aagggaaata tcagtcatcc gaagcgcctc
    12541 agaaattcaa cggcggcaag ggtttcggct tcctgcttgg aagcgccggt ggcttgtgtc
    12601 tcgcccacat tgcggacagc gacagccacc gtgaaacggg ctgcatgatc agggcctgaa
    12661 cgatccacca gccgatattc cggcatggcg cggcgattgc ccgcggccca ttcctgcagc
    12721 gccgatttgg ggtgcttgga ctggcccgcc ttgccgccga acacatcggc ccacaaccgc
    12781 cgcacgatgg cgcgggtggg ctcgaagcca agagtgagga agctggcgcc gatcagcgcc
    12841 tccatcacat cgcccagaat attgtcggaa tccgcgccgc catcatcgcg cgcctgcttg
    12901 cccaggcgga tgtgggggcc aagcccgatc tggcgggcaa cgcccgcgca agtggcgcgc
    12961 gagaccagca cgttcagccg ctgcgacagg cgcccctcgg cctcgccctc ggtctcgaac
    13021 agccagtcgg cgatgaccag gcccagcacc cggtcgccca ggaattccag ccgctgatag
    13081 tcgcgcgtct cgcccaggct gccatgggtg agggccgaga gccagtgcgg cgaggccggc
    13141 agcgcgccgc cgacgatccc ttccagcagc gcctgcgccg cctcatccag ggcgcgcgga
    13201 gacagcgaga gcttgccgct caaaacaggc ccccgatgcg gcgccagcgc gccgaggtga
    13261 accacgtcca cggcgcatac cactgcgcgc cgccatcggt ggaccacatg acgatggtgg
    13321 cccggccaac gagattggcc tgcggcacca tgccgatgcc gccttcgatc gtggagaagc
    13381 ggctgtccat cgaattgtcg cggtcatcgc ccatcaggaa caggctgcct tcgggcacca
    13441 gatagggggg cgtgttgtcc tgcggggtct gctcgaggtc gagcacgttg aagctcttgg
    13501 ccgggcggcc gtcctcggcg ggcagcgtct cgcgatattg cggatagcgg cagacatgcc
    13561 tgccattggg gctgccatcg ggcttcacca cctcatagtt gaggccttgc gtgtaatgcg
    13621 gctgatagca gctggtgttg gccgagacgg cgatctcgaa atcctcgatc ttcaccttgg
    13681 gaatggcctt gccgttgaga tagagcacgc cgttctgcat ctgcacgata tcgcccggca
    13741 ggccgatcac gcgcttgatc cagtcctgct cgttggcggg cggggccttg aagatcacca
    13801 catcgccgcg caccggctga tgggccagca tccgccccgg cagcagcggc accgaaaagg
    13861 gcagcgaata gctggaaaag ccatagggcc atttggccgc cagcagataa tcgccgttca
    13921 ccagccgggg cagcatcgat tcggaaggga tgttgaaagg cgagaagatg aagctgcgga
    13981 acaccgccac cagcaccacc agcttcagca ggaagacgag gaagctctcc tccttctcct
    14041 tgcgcgcggg ggcggcaggc gcggcgggcc cttcggcgcg ggcagggctg tcggcggggg
    14101 gcggtgtcgc aggctcggtc atgctcgcgt ccttgggcga ggggtggggg cgccgtcaag
    14161 cctgtgtgcg gcatttgcgc ggggaagtgg ccggtttcgg ggcaggcggt ggccccttgg
    14221 ggcacaatcg cgcccggcag atattcgtat ggggcggccg gggcggagca atctgccgct
    14281 tggcccgggc gcgcgggcag gg
//
LOCUS       DASRYE010000194         9161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_135092,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000194 DASRYE010000000
VERSION     DASRYE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135092"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFF98_10165"
     CDS             complement(<1..831)
                     /locus_tag="VFF98_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZU64037.1"
                     /translation="MVSRRDFGIGAALAAAGAAMGPAVRAAAYRAVPQRPSARVIVDN
                     DFAGDPDGLVALAHQLLTPKTRTVLVTASALDRQLMGAVAPERSAAIGADIAAELIRR
                     AGIAAPPPVVAGSEVLNAAAPEPSAAARAIVAEAMREDKLPLVFTCGGPLTNLAAALR
                     LEPAIAGRMRVIWIGGGTYPAGGWEYNLSADIAAARAVIEQSAVPLWQIPQAAYRQMQ
                     FSVAELADRLRPVSPFGAWLYDRFTSPPAFIDVAGAWPLGDSPTVLLSAIATESSRYQ
                     D"
     gene            1059..1607
                     /locus_tag="VFF98_10170"
     CDS             1059..1607
                     /locus_tag="VFF98_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64038.1"
                     /translation="MKKILASLIAVAALALTPAVAEAQHGGGGGGHGGGGGGGGGFHG
                     GGGGGGGGFRGGGGAGGGGYRGGAGGGWHGAPGGGWNGGGWRGGGGWRGGGWGGGWGW
                     DGWGWGWPGYYLGYYGDPWFWDYPAYPYYYYDYPPYPAYAEEAAPMAVPGQTTAAPPQ
                     TCGRWLWSDTDRQYHWMTNGCN"
     gene            1594..2043
                     /locus_tag="VFF98_10175"
     CDS             1594..2043
                     /locus_tag="VFF98_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZU64039.1"
                     /translation="MAVTRDRKIARGLLAAACLAALGLAGDAWAQQGYGPASAYGASG
                     STTPEAAMRALHEALHLSAQQEPAWQAYRARALQASQAQGRQQAAARLLPTLDAPHRM
                     DLVEAEMRAELAEQHNQAQLVKALYAQLSPNQRAIFDQRTLPRQSGQ"
     gene            complement(2051..2533)
                     /locus_tag="VFF98_10180"
     CDS             complement(2051..2533)
                     /locus_tag="VFF98_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU64040.1"
                     /translation="MQIRPATAADAPAIAAILMPTIREGGTYALDPQMREADALAYWL
                     GADRATFVAEEAGEILGTYYLRANQAGGGAHVANCGYMTAAAASGRGVAAAMCTHSLD
                     EARARGFRAMQFNFVVSSNARAVRLWERMGFAILCRLPEAFCHPTAGFVDALVMFRAL
                     "
     gene            complement(2575..3042)
                     /locus_tag="VFF98_10185"
     CDS             complement(2575..3042)
                     /locus_tag="VFF98_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU64041.1"
                     /translation="MPARRLDDHDLAILRLVQQDATIPQREIAERIALSAPAVQRRIA
                     RMEREGVIAGTAAMVEPAAVGLPITVVVELTLSDDRSPTVAAAKAFFRAAPEVQQCYW
                     VTGASGFVLILVAESLGAYEAISSRLFADNEMVRSYRSMVVLDRVKVGAQLPI"
     gene            3169..4110
                     /locus_tag="VFF98_10190"
     CDS             3169..4110
                     /locus_tag="VFF98_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU64042.1"
                     /translation="MTERKPLDALAGNAMLLLCLIWGTQQVAIKLAAPDMSPLLQVGL
                     RSGLSALLTLGLVLREGALVDLFGPYLRHGLAVGALFGLEFVCVGEGLRLSSASHVTI
                     FLYTAPVFAALWLGLLHRSERLRPVQWLGMALAFGGILLAFADSGASDAAAYPHMLLG
                     DALALAGGLAWAATMVLVRAGRLADAPASITLLYQLLGALVMACAMAFLLGESTVHLT
                     LRLAAAMAYQTLIVAFLSYLAWFALLRHYLASRLGILSFLTPVFGVLSGIAVLGERLG
                     PAFIAGALLILAGMLVVALPDLVAPRAPARPGARSGH"
     gene            4430..5818
                     /locus_tag="VFF98_10195"
     CDS             4430..5818
                     /locus_tag="VFF98_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU64043.1"
                     /translation="MSPAARPATGGDETLAHSLQPRHVTMISLGGIIGAGIFVGSGAA
                     IHLAGPCVLLTYALCGSLVFVIMRMLGEMAVARPGVGSFTGYAALGLGPWAGFVTGWL
                     YFYFWVVTIAVEAMAGANMLTGLVPLPAWAISAGLVAITAFINLLSVRTYGECEFWFA
                     ALKVATITGFIAVGAAYIFGFGPGVGHLAAVFAAHGGAIPLGAGAVFTAIPVVIFSMM
                     GSEVATIAAVETENPALNVVRAARTVTMRILFFYLGSVAVILAILPWDQVVPGFSPFA
                     SAMAVVHVPGASAFMQVVIFTAVVSCLNSAVYVTSRMLFEMAHRGDAPAACGHVSAAH
                     VPARAIVLSAIAGFAVVLTSIVSPTHVFVFLLQSSGAIILMVYMLIALSQIALRRKLE
                     AADEPLPVKVWLFPGLSYALVAGILAVLAMMALSPDQAEQVGLSAAVFALALAAYGLR
                     RRARHPAPARPA"
     gene            6169..8811
                     /locus_tag="VFF98_10200"
     CDS             6169..8811
                     /locus_tag="VFF98_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64044.1"
                     /translation="MLSTRNRWTVSAAALAIAAFAADITPAWAEEAASTAPDLKPQGD
                     IVVTATRRSESANKIPLAIHAMGGDTLKSLNITSLDSLVENLGNVRSASRGPGMSSIY
                     IRGLSTDTVSSQFAGTVGAFPNVALYLNDTPSSMPGRSLDIYPVDIQRVEVLAGPQGT
                     LFGANAMGGAIRYITNKPDPSKWAANVTGSVSSTYNAASSQGVNGFINIPLVKDHLAL
                     RVTAYVDHQGGYISNVPGTYQMPFNGNVGVAGQLPTGNPALVIRALQSCVGVSNCTGS
                     GYTAPIRQSISNAAYVGKDYNASQYQGVRVALGWHIDPDWSIDVMHMRQKLTSDGVFD
                     YEPGVGDLAVQKYNPNTLDDTFDMTTVEVAGRIGALSVLYAGSYVHHHAVQNADYASY
                     SNVGLYLPYYECDKGVYYNHTANAGNTCYTPSKHYTVNDTNRRWTNELRVVTPGDWRL
                     RGTVGVFYDVNKIYDNTDWYYSQPAAGYIYPRSANPVVNPNDASVKPVNDAFFNDTYR
                     RDSQFAVYGEASFDIIPKKLILTGGMRYYSESASLTGSSDGSFGGARGVYDPATGTYS
                     ASANPPQYYNYTNLATSLAGLSPAHYHGVIPKGNLTYKFDSGALVYATYSEGFRPGGF
                     NRKPCNTNTAACTALKYYVPDQVKNYEIGWKVPLFHHHLQLNAAAYIVRWTNIQMSVF
                     DQNISNQTFTANLLDARIHGIEGDVYWRPLPGFTLTSSFSYNDSKVTGYRQNVSTLVP
                     VGSPLAMSPKFQLTSTARKEFDMGRNYTSYIQLTWHHVSSSISSDVSNTDIRWTGGTV
                     TYNGVTVHNGDSIVPIPVSLPQAGYDTLSAAVGVHKDGWALDLFGENLTNARPELFKS
                     TSDGETRVTTSRPLTVGLRASFTY"
     gene            8932..>9161
                     /locus_tag="VFF98_10205"
     CDS             8932..>9161
                     /locus_tag="VFF98_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU64045.1"
                     /translation="MPSQTAPLSPAAPLEQARTRLEAHDFAGAASLARQALAAEPAQR
                     DALYLLAVAQRYGCDAAGALATLATLVEHHPA"
BASE COUNT         1445 a         3189 c         2967 g         1560 t
ORIGIN      
        1 atcctgatag cggctgcttt cggtggcaat ggcgctcagc agcacggtcg gactgtcgcc
       61 cagcggccag gcgccggcca catcgatgaa ggccggcggg ctggtgaagc gatcatacag
      121 ccaggcgccg aagggcgaga ccgggcgcag ccgatcggcc agttcggcca cggaaaactg
      181 catctggcga taggccgcct gcgggatctg ccagagcggc acggccgatt gctcgatcac
      241 cgcgcgggcc gcggcgatat cggccgaaag gttgtattcc cagccgcccg ccggataggt
      301 gccgccgcca atccagatca cccgcatgcg cccggcaatc gccggctcca gccgcagcgc
      361 ggcggccagg ttggtgagcg ggccgccgca agtgaacacc agcggcagct tgtcctcccg
      421 catcgcctcg gccacgatcg cccgcgccgc ggcagacggc tcgggcgcag cggcattcag
      481 cacctcgctg ccggccacca ccggcggcgg cgcggcaatg ccggcgcggc ggatcagctc
      541 tgcggcaatg tcggcgccga tggccgcgct gcgttcgggc gccaccgccc ccatcagctg
      601 gcgatccagc gccgaggcgg tgaccagcac ggtgcgcgtt ttgggcgtga gcagttgatg
      661 ggccagcgcc accagcccat cgggatcgcc ggcgaaatca ttgtcgacaa tcacccgcgc
      721 cgaggggcgc tgcggcaccg cccgataggc cgccgcgcgc accgccgggc ccatcgccgc
      781 ccctgcggcc gccagcgccg cgccgatgcc gaaatcgcgc cgtgaaacca ttccgtctct
      841 cccatattct aatttatcat acaatatggc ccggcatcgg aaaaggcaat gtctcccgat
      901 agatccaccc cgcagatggc ggcgcagtca gccgccgttc acgcaggcta tcgtattatt
      961 gcttattggt tcgcacgtgg cttggcggca cacagaatgc atgcctccgc ccgcacgaca
     1021 gggaacgcaa tccggacgga tcagggaagg aatcgcgcat gaaaaagatc cttgccagcc
     1081 ttatcgccgt ggccgcgctg gcgcttacgc cggcggtggc tgaggcccag catggcggtg
     1141 gcggtggtgg ccacggcggt ggtggcggcg gaggcggtgg ctttcacggc ggcggcggtg
     1201 gcggtggcgg cggttttcgc ggcggcggcg gggctggcgg tggcggctat cgcggcggcg
     1261 cgggcggcgg ctggcatggc gcgcccggcg gcggctggaa cggtggcggc tggcgcggcg
     1321 gcggtggctg gcgcggtggc ggctggggcg gcggctgggg ctgggatggt tggggctggg
     1381 gctggcccgg ctattatctc ggctattacg gcgatccgtg gttctgggat tatccggcct
     1441 atccctacta ttattacgat tatccgccct atccggccta tgccgaggaa gcggcgccca
     1501 tggccgtgcc cggacagacc acggcggccc cgccgcaaac ctgcggccgc tggctgtgga
     1561 gcgacaccga caggcaatat cactggatga ccaatggctg taactagaga ccgcaagatc
     1621 gctcggggcc tcctggccgc cgcctgcctc gccgcactgg ggctggccgg cgacgcctgg
     1681 gcccagcagg gctatggccc cgcctccgcc tatggcgcct ctggcagcac cacgcccgag
     1741 gcggcgatgc gcgcgctgca cgaggcgctg cacctgtcgg cccagcagga gccggcctgg
     1801 caggcctatc gcgcccgcgc gctgcaggcc tcgcaggcgc aggggcgcca gcaggccgcc
     1861 gcccgcctgc tgcccacgct ggatgcgccc caccgcatgg atctggtgga agcggaaatg
     1921 cgcgccgaac tggccgagca gcacaatcag gcgcagctgg tgaaggcgct ttacgcgcag
     1981 ctttccccca accagcgcgc catcttcgac cagcgcaccc tgccccggca gagcggccag
     2041 taaaccctgt ctaaagcgcg cggaacatca ccagcgcatc cacaaaaccg gctgtcgggt
     2101 ggcagaacgc ctccggcagc cggcacagga tggcaaagcc catccgttcc cacaggcgca
     2161 ccgcccgcgc attgctgctg accacgaaat tgaactgcat ggcgcgaaag ccgcgcgccc
     2221 gcgcctcgtc cagcgaatga gtgcacatgg ccgcggccac gccccgcccg ctggcggccg
     2281 ctgccgtcat atagccgcaa ttggccacat gggcgccgcc gcccgcctga ttggcgcgca
     2341 gataataggt gcccaggatc tcgcccgctt cctcggccac gaaggtcgcc cggtctgcgc
     2401 ccagccaata ggccagcgcg tcggcctcgc gcatctgcgg atcgagcgca taggtgccgc
     2461 cctcgcggat ggtgggcatc aggatggcgg caatggcggg cgcatcggcg gcggtggcgg
     2521 ggcggatctg catggcgcct gccttaggcc ccgcgcccag gccgccgcaa gccttcagat
     2581 cggcaattgc gcgcccacct tcaccctgtc gagcaccacc atcgagcgat aggagcgcac
     2641 catctcattg tcggcgaaca ggcgcgagga gatcgcctcg taggcgccca ggctttcggc
     2701 caccagaatg agcacgaagc ccgaggcgcc tgtcacccaa tagcattgct gcacttcggg
     2761 tgctgcgcgg aagaaggcct tggcggcagc caccgtgggc gagcggtcat cgctcagcgt
     2821 cagttccacc accacggtga tcggcaggcc caccgctgcc ggctccacca tggcggcggt
     2881 gccggcgatc accccttcgc gctccatccg ggcgatgcgc cgctgcaccg ccggggcaga
     2941 cagggcaatg cgctcggcga tctcgcgctg ggggatggtg gcatcctgct gcaccagcct
     3001 gaggatggcc agatcatggt cgtcgagacg gcgagcgggc attgcgaaat cctcttcccc
     3061 gatgagcgaa acttgcgcgg gcgccgcgca ataagagccc tcaacccgct tgtggcgagc
     3121 tatacgctat cactcaagcc cccttcccct gccatgaatg accgccccat gaccgaacgc
     3181 aagcccctcg atgcccttgc cggcaatgcc atgctgctgc tctgcctgat ctggggcacg
     3241 cagcaggtgg cgatcaagct ggccgcgccc gatatgagcc cgctgctgca ggtggggctg
     3301 cgctctggcc tgtcggcgct gctgacgctg gggctggtgc tgcgcgaagg ggcgctggtc
     3361 gacctgttcg ggccctatct gcgccacggg ctggcggtgg gggcgctgtt cgggctggaa
     3421 ttcgtctgtg tgggcgaggg cttgcgcctt tccagcgcct cgcatgtcac catctttctc
     3481 tacacagcgc cggtgttcgc cgccctgtgg ctggggctgc tgcaccgctc ggaacggctg
     3541 cggccggtgc agtggctggg catggcgctg gcctttggcg gcattttgct ggcctttgcc
     3601 gatagcggcg cgagcgatgc ggcggcctat ccgcacatgc tgctgggcga tgccctggcg
     3661 ctggcaggcg ggttggcgtg ggcggccacc atggtgctgg tgcgcgccgg gcggctggcc
     3721 gatgcgccgg cctcgatcac cctgctctac cagttgctgg gcgccctggt gatggcctgc
     3781 gccatggcct tcctgctggg cgagagcacg gtgcacctga ccctgcgtct tgccgcggca
     3841 atggcctatc agacgctgat cgtcgccttc ctgtcctatc tcgcctggtt tgcgctgctg
     3901 cgccactatc tggcctccag gctggggatc ctgtcgttcc tcacgcctgt gttcggggtg
     3961 ctttcgggca ttgccgtgct gggcgagcgg ctggggccgg ccttcatcgc cggcgcgctg
     4021 ctgatcctgg ccggcatgct ggtggtggcc ctgcccgacc ttgtggcgcc gcgcgcgccc
     4081 gctcgccccg gggcccgttc tggccattag ccaaactact gatcgccatt agcgggcggc
     4141 gcaagccccg gcccgccgcc aacggccggc ggcaaaccgg cgagaaaccg cgcttcgcac
     4201 ctgcacaaca gcccgttggc cccatcgcat gtgcagcatg gccggtccag ccgcggcggc
     4261 ctggcccgcg cgccctgccc gcaagcgcta ttgaccacaa gtgcgacact aaggttactt
     4321 gccttccgat ccagacaagc gacaggggtg gcgcggcggt gacggcattg atgaggcctt
     4381 tttcccattt ttccgcagta cattgccggc aaccggcaga ggcccgcgca tgagcccggc
     4441 cgcccggccc gccaccggtg gcgacgaaac cctcgcccat tcgctgcagc cgcgccatgt
     4501 cacgatgatc tcgctgggcg gcatcattgg cgccggcatc ttcgtcggct cgggcgcggc
     4561 gatccatctg gcgggcccct gcgtgctgct cacctatgcg ctgtgcggtt cgctggtctt
     4621 cgtcatcatg cgcatgctgg gcgagatggc agtggcgcgg cccggggttg gctcgttcac
     4681 cggctatgcg gcgctggggc tggggccgtg ggcgggcttt gtcaccggct ggctctattt
     4741 ctacttctgg gtcgtcacca tcgcggtgga agcgatggcg ggcgccaaca tgctcaccgg
     4801 ccttgtgccg ctgccggcct gggcgatcag cgccgggctg gtggcgatca ccgctttcat
     4861 caacctgctg tcggtgcgca cctatggcga gtgcgagttc tggttcgccg cgctgaaagt
     4921 ggccacgatc accggcttca tcgccgtggg cgcggcctat atcttcggct tcgggccggg
     4981 cgtgggccat ctggcggcgg tgtttgccgc ccatggcggc gccattccgc tgggcgcggg
     5041 cgcggtgttc accgccattc ccgtcgtcat cttctcgatg atgggcagcg aggtggccac
     5101 gattgccgcg gtggaaaccg aaaaccccgc gctcaacgtg gtgcgcgcgg cgcgcacggt
     5161 aacgatgcgc atcctcttct tctacctcgg ctcggtggcg gtgatcctgg cgatcctgcc
     5221 gtgggatcag gtggtgcccg gcttctcgcc ctttgcctcg gcgatggcgg tggtgcatgt
     5281 gccgggggca tcggccttca tgcaggtggt gatcttcacc gcggtcgtct cctgcctcaa
     5341 ttcggcggtc tatgtcacct cgcgcatgct gttcgagatg gcccaccggg gcgatgcgcc
     5401 ggcggcctgc ggccatgttt cggccgccca tgtgccggcc cgcgcgatcg tcctttcggc
     5461 cattgccggc tttgcggtag tgctcacctc gatcgtctcg cccacccatg tgttcgtgtt
     5521 cctgctgcaa agctcgggcg cgatcatcct catggtctac atgctgatcg cgctgagcca
     5581 gattgccctg cgccgcaagc tggaagcggc cgacgagccg ctgccggtga aggtgtggct
     5641 gtttccgggg ctgagctatg cgctggtggc gggcattctg gcggtgctgg cgatgatggc
     5701 gctgagcccc gatcaggccg agcaggtggg gctgtcggca gcggtgttcg cgctggcgct
     5761 ggcggcctat gggctgcgcc gccgcgcccg ccacccggca ccggccaggc ccgcatgagc
     5821 tgataccagc cccctcgccc gcccctgcga ccggcggccg aggcgcaaca tatttgccag
     5881 ttacacaagc attggggagt ttgtgaccat gactgaagcc tgccgccaac attggccaac
     5941 accgcaaggg cgcatccggc cctagcgccc ttgcggccgg cccgcccggc ccttcccttc
     6001 cgcttcctcc gatccctcgc gcgcagtgct gcgcgtgaag gctggcgcgc gcccggcgat
     6061 gagggcgcgc gcgcattatc aagccaaaaa caaaaagcga cctcaggagc gaacacgcga
     6121 ggccagactg caactggccg cattgatgca aaacatgggg gtacacacat gctttcgaca
     6181 cgaaaccgat ggaccgtttc ggccgcggcg ctggccattg ccgcctttgc cgccgacatc
     6241 acgcccgcct gggccgagga agcggcatcg acagcgccag acctgaagcc gcagggcgat
     6301 atcgtggtga cggccacccg ccgttcggaa tcggccaaca agatcccgct cgccatccat
     6361 gcgatgggcg gcgacacgct gaaaagcctc aacatcacca gccttgattc gctagtggag
     6421 aacctgggca atgtgcgcag cgcctcgcgc gggccgggca tgtcctcgat ctacatccgc
     6481 ggcctgtcga ccgacaccgt cagctcgcag tttgccggca cggtgggcgc cttccccaac
     6541 gtcgcgctct atctgaacga cacgcccagt tcgatgcccg gccgcagcct cgatatctat
     6601 ccggtggaca tccagcgcgt ggaagtgctg gccggccccc agggcacgct gtttggcgcc
     6661 aatgccatgg gcggcgccat ccgctacatc accaacaagc ccgatcccag caaatgggcg
     6721 gccaatgtca ccggcagcgt ttcctccacc tataatgccg ccagcagcca gggggtgaac
     6781 ggcttcatca acatcccgct ggtgaaagac catctggccc tgcgcgtaac cgcctatgtc
     6841 gatcatcagg gcggctatat cagcaatgtg ccgggcacct atcagatgcc tttcaacggc
     6901 aatgtgggcg tggcgggcca gttgcccacg ggcaatccgg cgctggtgat ccgcgcgctg
     6961 caatcctgcg tgggggtcag caactgcacc ggctccggct ataccgcgcc catccgccag
     7021 tcgatcagca atgccgccta tgtgggcaag gattacaacg ccagccagta tcagggcgtg
     7081 cgcgtggcgc tgggctggca tatcgacccc gactggtcga tcgatgtgat gcacatgcgc
     7141 cagaagctga ccagcgacgg cgtgttcgat tatgagccgg gcgtgggcga tctggcggtg
     7201 cagaaataca accccaacac gctcgacgac acgttcgaca tgacgacggt ggaagtggcc
     7261 gggcgcatcg gtgcgctcag cgtgctctat gccggctcct atgtgcacca ccatgcggtg
     7321 cagaacgccg attatgccag ctattccaac gtcggcctct atctgcccta ttacgaatgc
     7381 gacaagggcg tttactacaa ccacaccgcc aatgccggca acacctgcta cacgcccagc
     7441 aagcactaca cggtgaacga caccaaccgc cgctggacca acgagctgcg cgtggtcacc
     7501 cctggcgact ggcgcctgcg cggcacggtg ggcgtgttct acgacgtcaa caagatctac
     7561 gacaacaccg actggtatta ttcgcagccg gccgccggct acatctatcc gcgctcggcc
     7621 aatccggtgg tgaaccccaa cgatgccagc gtgaagcccg tcaacgatgc cttcttcaac
     7681 gacacctacc ggcgcgacag ccagttcgcc gtctatggcg aggcctcgtt cgacatcatc
     7741 cccaagaagc tgatcctcac cggcggcatg cgctattatt cggaaagcgc ctcgctcacc
     7801 ggttcgtcgg atggcagttt tggcggcgcg cgcggggtct atgatccggc caccggcacc
     7861 tattcggcca gcgccaaccc gccgcaatat tacaactaca ccaatctggc gacctcgctg
     7921 gcgggcctct ctccggcgca ttaccacggt gtgatcccca aggggaacct gacctacaag
     7981 ttcgattccg gcgcgctggt ctatgccacc tattccgaag ggttccgccc cggcggcttc
     8041 aaccgcaagc cctgcaacac caacaccgcc gcctgcacgg cgctgaaata ttatgtgccc
     8101 gatcaggtga agaactatga gatcggctgg aaggtgccgc tgttccacca ccatctgcag
     8161 ctcaacgcgg cggcctatat cgtgcgctgg acgaatatcc agatgtcggt gttcgaccag
     8221 aacatctcga accagacctt caccgccaac ctgctggatg cgcgcatcca tggcatagag
     8281 ggcgatgtct actggcgccc gctgcccggc ttcaccctca ccagctcctt ctcctataat
     8341 gattccaagg tgaccggcta tcgccagaac gtctccacgc tggtgccggt gggcagcccg
     8401 ctggcgatga gcccgaaatt ccagctgacg agcaccgccc gcaaggaatt cgacatgggc
     8461 aggaactaca cctcctatat ccagctcacc tggcaccatg tgtcctccag catctcctcg
     8521 gatgtctcga acaccgacat ccgctggacg ggcggcaccg tcacctacaa tggcgtgacc
     8581 gtgcataatg gcgattccat cgtgccgatc cctgtctcac tgccgcaggc cggctacgac
     8641 acgctgtcgg cagcggtagg cgtgcacaag gatggctggg cgctggatct gtttggcgag
     8701 aacctcacca atgcacggcc agaactgttc aagagcacca gcgatggcga aacccgcgtg
     8761 accacctcac ggccgctgac agtgggcctg cgcgcctcct tcacctactg acggaccctg
     8821 cacctcaccg gggcggcatc ggcctgccgg cgccgccccg gctttttttg agggcacccc
     8881 ccttcctgcc ccttgcggca ttcccttcct ccggcaagga tcgcctctgc catgcccagc
     8941 cagacagccc ccctctcgcc cgccgccccg ctggaacagg cgcgcacgcg gctggaggcc
     9001 catgattttg ccggcgcggc cagcctggcc cggcaagcgc tggcggcaga accggcccag
     9061 cgcgatgcgc tctatctgct ggccgtggcg cagcgctatg gctgcgatgc ggccggcgcc
     9121 ctggccacgc tcgccacgct ggtggagcac caccccgcct a
//
LOCUS       DASRYE010000195         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_98767,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000195 DASRYE010000000
VERSION     DASRYE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98767"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VFF98_10210"
     CDS             complement(<1..156)
                     /locus_tag="VFF98_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZU64046.1"
                     /translation="MRPTPAPQTYRVKSFGCQMNVYDGERMGELLADQGLHPAAEGDE
                     ADLVVLNT"
     gene            complement(238..1101)
                     /gene="hemF"
                     /locus_tag="VFF98_10215"
     CDS             complement(238..1101)
                     /gene="hemF"
                     /locus_tag="VFF98_10215"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZU64047.1"
                     /translation="MTDWTAHTDRARHWFEALRDAICAAFEAIEREAGSDAAFQYTPW
                     TRDAVGSEGGDTGGGTRGLMKGKVFEKVGVNISTVHGAFSPQFAASINGASVDNPGFT
                     ATGISLVAHMANPHVPAVHMNTRFLTTTKAWFGGGADLNPPIPYAEDTADFHAAFKAC
                     CDGHGADYYDRFRAWADEYFFIPHRGVARGVGGIFYDHLECADDEAWEANFAFTRAVG
                     ETFLAIFPQLVRRRMGLAFSPAEKARQLEWRGRYAEFNLVYDRGTIFGLKTGGNVEAI
                     LMSLPPEATWS"
     gene            1168..1854
                     /gene="pdeM"
                     /locus_tag="VFF98_10220"
     CDS             1168..1854
                     /gene="pdeM"
                     /locus_tag="VFF98_10220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HZU64048.1"
                     /translation="MVRLSFAPFSFAGQEWAMAGPRALYWPQERALLVADLHLEKASW
                     YAAGGQMLPPYDSRETLERLGAVAAAADARRIFCLGDNFHDDAGPTRLEAGAAALLGA
                     LAQGRELVWIAGNHDAQMAGGLPGTCAPLLEVAGLALVHEARPFAQGPEISGHFHPAL
                     TLEARGRRLRRPCCVASENRLILPAFGALTGTMAAADPAIVAALQPARAIDALVAVAE
                     RVARYPLWRG"
     gene            1964..2485
                     /gene="infC"
                     /locus_tag="VFF98_10225"
     CDS             1964..2485
                     /gene="infC"
                     /locus_tag="VFF98_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZU64049.1"
                     /translation="MMAPPVKSGPRYNEMINVPRVRVIDDEGENLGILYTRDAIAQAF
                     EAGLDLVEVSPNADPPVCKFLDVGKFRYEAQKKANLARKTQKTQEIKEIKMRPNIDDH
                     DYDVKMKAMQRFIGEGDKVKVTLRFRGRELSHQQLGMDVLRRVQEDMAEVAKVEAYPR
                     MEGRQMLMVLSPK"
     gene            complement(2722..3159)
                     /locus_tag="VFF98_10230"
     CDS             complement(2722..3159)
                     /locus_tag="VFF98_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZU64050.1"
                     /translation="MHRPIDIEALCAQRGLRITEQRRVIARVLSESTDHPDVDKLHER
                     ASAIDPGISIATVYRTVRLFEEAGILDRHDFGDGRARYEAAPEAHHDHLIDVENGTVI
                     EFVDPELEALQRQIAERLGYRLVDHRLELFGVKLERGEQPSRK"
     gene            complement(3288..3728)
                     /locus_tag="VFF98_10235"
     CDS             complement(3288..3728)
                     /locus_tag="VFF98_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HZU64051.1"
                     /translation="MSIPQNDLNETLITLTSDIVAAHVSNNSVSVEDLPTLISNVYGA
                     LAGLGQEAPVEETPPEPAVSIRASVKPDHIVCLEDGKKLKMLKRHLMTHYNLTPEQYR
                     ARWNLPADYPMVAPNYAEKRRELAKKIGLGRKPGPRRGRKPKAA"
     gene            complement(3891..4376)
                     /locus_tag="VFF98_10240"
     CDS             complement(3891..4376)
                     /locus_tag="VFF98_10240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU64052.1"
                     /translation="MTMPAIAPEDIDRIMAVMASAFEPHWGEAWTRPQVESALSSGYC
                     AYFLIGPEGGAPDPGAAVAGFALVRSLFDEAELLLFAIDAAWQRQGLGRRLLEDVLAQ
                     ARSAGIRRMLLEVRAGNKAEQLYRTLGFAPIGRRAGYYRAQNGERLDAITLSCTVSER
                     E"
     gene            complement(4388..5038)
                     /gene="tsaB"
                     /locus_tag="VFF98_10245"
     CDS             complement(4388..5038)
                     /gene="tsaB"
                     /locus_tag="VFF98_10245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZU64053.1"
                     /translation="MSSAPPRTLAIECTTQACSVALFEGDRLVAGDWRLLGRGHAEAL
                     VPMIAALPGKGQAQRIAVGRGPGSFTGVRIGLAAARALALAWEAELAGYPTLALIAAM
                     AQAQVPATPVLVATTGGHGEWFVQNFDSAGLPAGPLASQKPEIAAVDTTPLVAGSEAE
                     ALVARRGPMPGQGTALPLWPDARALMALPAALFTAETEPLYGRAPDARLPGGVLPA"
     gene            complement(5056..5637)
                     /locus_tag="VFF98_10250"
     CDS             complement(5056..5637)
                     /locus_tag="VFF98_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZU64054.1"
                     /translation="MFIETETTPNPATLKFLPGQQVMVAGTREFVDEEAAAASPLAEA
                     LFTLGDVTSVFFGQSFISVTAGPGSDWAALKPQVVSVLLDHFVSGAPLFAGGSAAGIA
                     VPAEADEAVPGDDPADADIIEQIKDLIETRIRPAVANDGGDVVYRGFREGIVYLQMQG
                     ACSGCPSSSATLKNGIESLIKYYVPEVTEVRAA"
     gene            5754..>6129
                     /locus_tag="VFF98_10255"
     CDS             5754..>6129
                     /locus_tag="VFF98_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64055.1"
                     /translation="MLELISLHVPKCAGSSLRAALAAAYAGAGEGAFWADYADRPGNP
                     ASAFQLDPAGYHAAMAGWAPPVGARAVHGHFHPGKYRRHGAGALKAVFLRHPVSRLIS
                     HYRFWQALPRHGHLLHDYMLDTG"
BASE COUNT         1003 a         1951 c         2096 g         1079 t
ORIGIN      
        1 ggtgttgagc acgacgagat cggcctcgtc cccctctgcg gccggatgga ggccctgatc
       61 ggccagcagc tcgcccatgc gttcgccatc atagacgttc atctggcagc cgaagctttt
      121 cacgcgatag gtttgggggg caggggtggg gcgcataatc cgccggccca tacgccaatt
      181 gcgggcggat ttgaaggggg gagtgggcgg tgtgcgccac tcccctcccg gcagtgctca
      241 gctccaggtt gcctcgggtg gcaggctcat caggatggct tccacattgc cgccggtttt
      301 gaggccaaag atcgtgccgc gatcgtaaac caggttgaat tccgcatagc ggccgcgcca
      361 ttccagctgc cgggcctttt ccgccgggct gaaggccagc cccatgcgcc ggcgcaccag
      421 ctgcgggaag atggcgagga aggtttcgcc cactgcccgg gtgaaggcaa agttggcctc
      481 ccacgcctcg tcgtcggcgc attcgaggtg atcgtagaag atgccgccca caccgcgcgc
      541 caccccccgg tgcgggatga agaaatattc gtccgcccag gcccggaagc ggtcgtagta
      601 atccgcgcca tggccatcgc agcaggcctt gaaggcggcg tggaaatcgg cggtgtcctc
      661 ggcatagggg atcggcgggt tgagatccgc gccgccgccg aaccacgctt tggtggtggt
      721 gaggaagcgc gtgttcatgt gcacggcggg cacatgcggg ttggccatat gcgccacaag
      781 gctgatgccg gtggcggtga agccggggtt gtccacgctg gcgccgttga tgctggcggc
      841 aaattgcggg ctgaaggcgc catgcacggt ggagatgttg acgcccacct tctcgaacac
      901 cttgcccttc atcagcccgc gggtgccgcc gccggtgtcg ccgccttcgc tgcccacggc
      961 gtcgcgggtc cagggggtgt actggaaggc ggcatcgctg cccgcctcgc gctcgatcgc
     1021 ctcgaaggcg gcgcagatcg catcgcgcag cgcttcgaac cagtggcggg cgcggtcggt
     1081 gtgggcggtc cagtcggtca aggcagtctc tccatcggcg ttgcccggcg cttgccattg
     1141 ccggcggctt gcggcaaggg cgctggcatg gttcgccttt cgttcgcgcc gttttccttt
     1201 gccgggcagg aatgggccat ggcaggcccg cgcgcgctgt attggccgca ggagcgcgcc
     1261 ctgctggtgg ccgatctgca tctggaaaag gccagctggt atgccgccgg cggccagatg
     1321 ctgccccctt acgacagccg cgagacgctg gagcggctgg gcgcggtggc agcggcggcg
     1381 gatgcgcggc ggattttttg cctgggcgac aattttcacg acgatgccgg ccccacccgg
     1441 ctggaagcgg gcgcggccgc gctgctgggc gccctggcac aggggcgcga gctggtgtgg
     1501 attgccggca accatgatgc gcagatggcc ggcggcctgc ccggcacttg cgcgccgctg
     1561 ctggaagtgg ccggcctggc gcttgtgcat gaagcccggc cctttgccca aggcccggaa
     1621 atatccggcc atttccaccc cgccctcacg ctggaggcac gcgggcggcg gctgcgccgg
     1681 ccctgctgcg tggccagcga aaaccgcctg atcctgcccg ccttcggcgc cttgaccggc
     1741 acaatggccg ctgccgatcc ggccattgtg gcagcgctgc agccggcgcg ggcgatcgat
     1801 gcgctggtgg cggtggcaga gcgggtggcg cgttatccat tatggcgcgg ttaaggcggc
     1861 gcgcggcgcg aaaattgccc ttccacgctc tcgcgattcg ctttaagagc gcgcaagttc
     1921 tgtaacctca ggagatacga ctatagctcc cccaccccgg cgcatgatgg cgccccccgt
     1981 caagagcggg ccacgctata atgagatgat caacgtgccc cgcgtgcgcg tgatcgacga
     2041 cgagggcgaa aacctcggca tcctctacac gcgcgatgcg atcgcccagg ccttcgaggc
     2101 cgggctcgat ctggtcgaag tgtcgcccaa tgccgatccg ccggtgtgca agttcctcga
     2161 tgtcggcaag ttccgctacg aggcgcagaa gaaggccaac cttgcccgca agacccagaa
     2221 gacgcaggag atcaaggaga tcaaaatgcg tccgaacatc gacgatcatg attatgacgt
     2281 gaagatgaag gccatgcagc gcttcattgg cgagggcgac aaggtgaagg tgaccctgcg
     2341 cttccgtggc cgcgaactgt cgcaccagca gctgggcatg gatgtgctgc gccgcgtgca
     2401 ggaagatatg gccgaagtgg ccaaggtgga agcctatccg cgcatggaag gccgccagat
     2461 gctgatggtg ctgtcgccca agtgaggcgc tgaggccaaa gagctctccc ccgctatttc
     2521 acgccaaccc gggggaatct acgaaggggc gccggccagg ccctggcaac agggcagggc
     2581 tggcgccctt tgtgctggcg gtgctggggc gcggcggctc tgcgcaaaag aggcggctct
     2641 gcgcaaaaga agcgcgaaag gtttccccgt cgcgcttctg tgttgctttt tccggaaggg
     2701 cgctggcttt atttgcaggc cttatttgcg ggaaggctgc tcgccccgct ccagcttcac
     2761 gccgaacagt tccagccggt ggtccaccag gcgatagccc agcctttcgg cgatctggcg
     2821 ttgcagcgct tccagctcgg gatcgacgaa ttcgatcacc gtgccgtttt ccacatcgat
     2881 cagatgatca tgatgggcct cgggcgcggc ttcgtagcgg gcgcgcccgt cgccgaaatc
     2941 gtgccggtcg aggatgccgg cttcctcgaa caggcgcacg gtgcgataga ccgtggcgat
     3001 cgagatgccc ggatcgatgg ccgaggcgcg ttcgtgcagc ttgtccacgt cggggtgatc
     3061 ggtgctttcg gaaagcacgc gggcgatcac gcggcgctgt tcggtgatgc gcaggccccg
     3121 ctgggcacac agggcttcga tgtcgatagg ccgatgcacg gggataaacc ttcaattaaa
     3181 acgccgcgtc tctatggacg cggcgctatg gttaggcaac cgggctatgg gagcaatccg
     3241 gtataaaggg cgatgcggcc ttgcggggtg catcgcccgg caggccatca ggccgccttg
     3301 ggcttgcggc cacggcgcgg gccgggcttg cggccaaggc caatcttctt ggccagttcg
     3361 cggcgcttct cggcatagtt gggggccacc atcggatagt cggccggcag gttccagcgc
     3421 gcgcgatact gttccggggt gaggttatag tgcgtcatca ggtggcgctt cagcattttc
     3481 agcttcttgc catcttccag gcacacaatg tgatcgggct tgaccgaagc gcggatcgac
     3541 acggccggtt ccggcggggt ctcttccacc ggggcttcct ggcccagacc tgccagcgcg
     3601 ccatacacat tgctgatcag cgtgggcaga tcttccaccg agacgctgtt gttgctgacg
     3661 tgcgcggcga cgatgtcgga ggtgagagtg atcagcgttt cgttcaaatc gttctgcgga
     3721 atgctcatga gagtgcctct tgctttatgc gacccgttgt tatcgttagt tgccgcgatc
     3781 ccgccctgat cgccgcggtt ctctcatgtc gatttctgcg aaatattcaa gtagtatttt
     3841 acgcaatgta ccaaagggag aagtcggtgc tcatctgccg gggcgttctt tcattcccgt
     3901 tcagatacag tacagctcag agttatcgca tcgagtctct cgccattttg cgcccgataa
     3961 tagcccgcgc ggcgcccgat aggggcaaat ccgagggtgc gataaagttg ttctgcctta
     4021 ttgcccgccc ttacttcgag cagcatgcgc cggatgccgg cagaccttgc ctgcgccagc
     4081 acatcttcaa gcaggcgccg ccccaggcct tggcgctgcc aggccgcatc tatggcaaac
     4141 agcagcagtt cggcctcgtc gaacaggctg cgcaccaggg caaagccggc caccgccgcc
     4201 cccgggtccg gcgcgccgcc ctctggcccg atcaggaaat aggcgcaata accgctggat
     4261 agcgcgcttt ccacctgcgg ccgggtccac gcctcgcccc aatgcggctc gaaggcgctg
     4321 gccatcaccg ccatgatccg atcgatatct tccggggcaa tggcgggcat ggtcatggaa
     4381 aaaacgctca ggccggcagc acgccgcccg gcaggcgcgc atccggcgcg cggccataaa
     4441 gcggctcggt ttcggcggtg aacagggcgg ccggcagggc catcagcgcc cgggcatcgg
     4501 gccacagggg cagggcggtg ccctgcccgg gcattgggcc gcgccttgcc accagcgctt
     4561 cggcctcgct gccggccacc agcggcgtag tgtccacagc cgcgatttca ggcttttgcg
     4621 aggcaagggg gccagcgggc aggcccgcgc tgtcaaaatt ttgcacgaac cattcgccat
     4681 ggccacccgt ggtggccacc agcaccggcg ttgcgggcac ttgcgcctgc gccatggcgg
     4741 caatcagcgc cagcgtggga tagccggcaa gctctgcctc ccaggccagc gccagcgcgc
     4801 gggcggcggc aaggccaatg cgcacgccgg tgaagctgcc cgggccccgg cccacggcaa
     4861 tgcgctgcgc ctggcccttg ccgggcaggg cggcgatcat cggcaccagc gcttcggcat
     4921 ggccgcggcc cagcaggcgc cagtcacccg cgaccagccg gtcgccctcg aacagcgcca
     4981 ccgagcaggc ctgggtggtg cattcgatgg ccagcgtgcg aggtggggcg ctgctcatgc
     5041 gctcagtggc ccggctcagg ccgcgcgcac ttccgtcact tcgggcacat aatatttgat
     5101 aaggctttca atgccgttct tcagcgtggc cgaagaagag gggcagcccg aacaggcccc
     5161 ctgcatctgc agatacacaa tgccttcgcg aaaaccgcga tagacgacat cgccgccatc
     5221 attggcaacg gcggggcgga tgcgggtttc gatgagatcc ttgatctgct cgatgatgtc
     5281 ggcatcggca gggtcgtcgc ccggcacggc ctcgtccgct tcggcgggaa cggcgatgcc
     5341 ggccgcgctg ccgcctgcaa acagcggtgc gcccgaaacg aaatgatcga gcagcaccga
     5401 gaccacctgc ggcttcagcg ccgcccagtc gctgcccggc ccggcggtga cggaaatgaa
     5461 gctctgcccg aagaacaccg aggtgacatc gcccagcgtg aacagcgctt cggccagcgg
     5521 cgaggcagcg gcagcctcct cgtcgacgaa ctcgcgcgtg ccggccacca tcacctgctg
     5581 gccgggcagg aatttcagcg tggcgggatt cggggtggtt tcggtctcga tgaacataag
     5641 gcccatgtag gcattcgcgg gggagggtca aggggcggcg gggctcggaa atggaggcat
     5701 taatggaggc attggcggct gcgctgcggg ctggggcatg gcatggggca gggatgctgg
     5761 aactgatctc gctgcatgtg cccaaatgcg ccggctcgtc cctgcgcgcg gcgctggcgg
     5821 cggcctatgc gggcgccggc gaaggcgcct tctgggccga ttatgcggac aggcccggca
     5881 atccggccag cgccttccag ctggatccgg ccggctatca cgcggcgatg gcgggctggg
     5941 cgccgccggt gggcgcgcgg gcggtgcatg gccatttcca ccccggcaaa tatcggcgcc
     6001 acggggcagg ggcgctgaag gcggtgtttt tgcgccatcc ggtcagccgg ctgatctcgc
     6061 attatcgttt ctggcaggcc ttgccccgcc atggccatct gctgcatgat tacatgctgg
     6121 atacagggc
//
LOCUS       DASRYE010000196         8990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_183379,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000196 DASRYE010000000
VERSION     DASRYE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183379"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFF98_10260"
     CDS             complement(<1..548)
                     /locus_tag="VFF98_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64056.1"
                     /translation="MTEAEADPSAQAGMQAGRQAGKGHRPQALRVGLWGAGVMAVALG
                     CAWAEREGIARHLIDSEARRLGLEAAYRIEEIGPGGAVLADLRLGDPSHPDLYVRRIE
                     IGTGFGQGLPAIARLRLEGVRLRGAIKGGRLGFGALDRLLAMRSSAGGGLPDVALDVA
                     DARASITGEAGPIGVALAGAGA"
     gene            755..2071
                     /gene="chrA"
                     /locus_tag="VFF98_10265"
     CDS             755..2071
                     /gene="chrA"
                     /locus_tag="VFF98_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HZU64057.1"
                     /translation="MPSAKADFAELLRVSARIGCLSFGGPAAQIALMHREMVEERGWI
                     SEEQYLHALNFCHLLPGPEAQQLAIWIGWKRHGVPGGLVAGLLFVIPGALVILALSVL
                     YGMAAQLAWFAALFLGIKAAVLAIVAQALLRLATRALATPLRKAAAAASFLALWLFNA
                     PFPLLVAAAALAGWLAARLRPALLGLPALPAASTAPGTPPPWRASLRAALAGAVLWAA
                     PLVAVHRLLGPGHVLWRIGLFFSKLAVVSFGGAYAVLAYMAQQAVSAQHWLSAAEMAD
                     GLGLAETTPGPLIMVTQFVGYLAAYRAPAPFSPQVAGLLGAGLVTWVTFTPCFLWVFT
                     LAPWIERLERARQLQGALALVTAAIVGVIGHLGSWFALQVLFRQLRPVALGPAHITLP
                     VAASFDWRAGLIALAAAWLVLRGRWGLIPVLAMAAAAGWLLAALPG"
     gene            complement(2091..3353)
                     /locus_tag="VFF98_10270"
     CDS             complement(2091..3353)
                     /locus_tag="VFF98_10270"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HZU64058.1"
                     /translation="MAIIVGGIGASHSPTIGFAKDTHKQADPAWAPIFAGFAEIREWV
                     RRQRIDVIFTIYNDHITSFFFDHYSAFCLGIDDQYVAADEGGGARAVAPARAHAALAR
                     HIGAALVADEFDMSFFQGKPLDHGCLSPLGMLADAEGPWQGAIVPLQVGVLQLPIPNA
                     RRCYKLGKSLRRAIESYPEDLRVAVMATGGLSHQVHGERAGFLNPAWDEEFLDLLESA
                     PEKLVEMRLAEYALLGGMEGAEVIMWLIMRGALSDKVRCTHRATYAPSVTNIATLVFE
                     DTGTAPDEAANAAYRAHIGYELEGAQNVPGTHPFTLERSLANYRLNDFLHRLIEPAHR
                     ARFLGDFDGLAAQFGLSAEECALVKGHQWLEMVRRGVSFFVLEKMAAVLGLSNPELYA
                     SFRGETLEEFLATRQQPITYSVAGLVEA"
     gene            3469..4362
                     /locus_tag="VFF98_10275"
     CDS             3469..4362
                     /locus_tag="VFF98_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64059.1"
                     /translation="MRWRLDDLEVLCAIAEAGGITGAARRLDLPKSTVSKLLSRLEQG
                     LGVRLIERSSRRVAVTSEGEAFIARASLILDMAHEADAMMQGLLAVPTGRVTLAAPAA
                     FAREVLTPRLHDFARRYPQILLDLVVTSHPAEGIGERCDMAVVAGMQPDSSLSQKVLL
                     GGQLIWITSPAYAAAHDLGGPGAEDLSHLMIWETRYARHGVDVHEGGLARHLPLPRKA
                     MRVNDPICVREAVIGGMGVSFLPERYCIGALADGRLQQVWPHLRFDREAARLALLYPG
                     SKLLSPRFRAVAGFLEEICAR"
     gene            complement(4403..6622)
                     /locus_tag="VFF98_10280"
     CDS             complement(4403..6622)
                     /locus_tag="VFF98_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64060.1"
                     /translation="MKRSVFLANCLVGAAWFPAVAYAQNAGTDAATGQGDIVVSARQR
                     AEKLQEVPVSVTAFTEKQLRDAGVKRVEDFIHMTPNITFVNSQNPGTSFITIRGLTQN
                     RNQPAPVAIVVDGVLQSSDREFAAGLLDINDIEVVRGPQGALYGRNATGGAIIINTKK
                     PTNLTEGHVEAGAASGDDYYGEATLSGPVIRDKLLGRISVRVQNREGYYDNIVLHQKV
                     DPLKDVIIQSRLVYNATDRLTFDFKANYNRTTAGALNLTYQPANLNADGVTLNTANPF
                     DYSRGDANQVNRTFYANNIGISKRTIASASLRSAYEFDFATLSSVTAFTHVEEGFASD
                     QYPYTATRDAMVNGVMVGDGVQSQWTSSDAISEEIRLTSRANQRLRWMVGAYYVGNNH
                     FISTSTSEDLGYGLVILEKVPQFNDPRIPTLSFLADDNHDRAWALFGNAEYDLTRRLE
                     LGVALRYDEDYRRQIVSPYNTAGSGGDVNNAHFGKLQPKFTLKYKALDNINLFTSWGQ
                     GFRSGQFNQNGVGAAAATIGLNGVSDVAKQEVTSSFEAGFKSSWLGNHLTVDGTYFHT
                     AARNVQYFVFVSSISAQVLVNIDKVAINGGELEAALHFGGFRLDVGAGITDATIKAYS
                     LSPTDVGRKAPFVPNKTVQVGAQYTMPVSGALNLTGRIDYELRGKQFWSPENETARKD
                     LNLVNLRLTLADSKGRWEMAGFVKNLTDLKYNEEYGSGGFAYAANPRSYGMSLRYNY"
     gene            complement(6819..8333)
                     /locus_tag="VFF98_10285"
     CDS             complement(6819..8333)
                     /locus_tag="VFF98_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HZU64061.1"
                     /translation="MALSGMMYIAGQPCEGSGPAFHAQAAVSGALLAPAYREASAAQV
                     AAACLAAEQACDAYRGHPLAARAAFLEAVAEEILALGDELIVRCMAETGLPRARLEGE
                     RGRTMGQLRLFAEMLRKGEGLGERHDPALPDRAPAPRPDLRMQRVPLGPVAVFGASNF
                     PLAFSVAGGDTASALAAGCPVIVKAHPAHPGTSELVGLAITRAAARSGMPAGVFSLLH
                     GAGHAVGAALVADGHVQAVGFTGSRAGGLALMRLVQQRPVPIPVYAEMSAINPVVLMP
                     GALAARAEAIGQAFVASLVLGAGQFCTNPGLILGVAGPDFDRFEAAACAALRQAPAAT
                     MLTPAIAAAYGARLALLEGRAEVALLARGAAGEGGTCAAALFATDAQAFLADAALHEE
                     TFGAAALLVRCADQAELLAVLRALEGQLTAAVHAVPADYAAVRQLMPVLERKVGRILF
                     DGFGTGVEVAAAMVHGGPFPASSDSRTTSVGTLAIERFWRPVCYQDMPAELADG"
     gene            complement(8373..>8990)
                     /locus_tag="VFF98_10290"
     CDS             complement(8373..>8990)
                     /locus_tag="VFF98_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZU64062.1"
                     /translation="EAGLCALSCTPSHAWVAPAGGTRPLFGTNPIAFAWPRAGALPFV
                     FDLATSAAARGEIELHRLAGLPIPAGWALDGDGQPTTDAAAAMAGAMLPFGGHKGSAL
                     AAMVELLAGPLIGDLTSAASLAADAGRGASPLGGQLILALDPAGFLGGAAAGALAQAE
                     ALFAGMRDQGARLPGERRHAARARSLREGMAVDGALLARIRALLD"
BASE COUNT         1401 a         3166 c         2979 g         1444 t
ORIGIN      
        1 aaagctcctg cccccgccag cgccacgccg atgggcccgg cctcgccggt gatgctggcc
       61 cgggcatcgg ccacgtccag cgccacatcg ggcaggcccc cgccggcact gctgcgcatg
      121 gccagcagcc ggtccagcgc accaaagccc agccgcccgc ccttgatggc cccgcgcagc
      181 cgcacccctt ccagccgcag ccgggcgatc gccggcaggc cctggccaaa ccccgtgccg
      241 atctcgatcc ggcggacata gagatcggga tggctgggat cgcccagcct gaggtcggcc
      301 agcaccgcgc cgccaggccc gatctcctcg atgcgatagg ccgcctccag ccccagccgc
      361 cgggcctcgc tgtcgatcag atggcgcgcg attccctcgc gctcggccca ggcacagccc
      421 agcgccacag ccatcacccc cgcgccccac agccccacgc gcagcgcctg cggccgatgc
      481 cccttgccag cctgccggcc ggcctgcatg ccagcctgcg ccgacggatc cgcttctgcc
      541 tcggtcatgg cgctgttgtt gccggcctgc ccgcgaaagg caatgccacg cgcgaccaat
      601 gcccgcctgc cagcggtaat ccggccggcc cggttgccgc aagggcccgc ctgcgctagg
      661 agcctacctg ccgaacaggc ccccccttcg cccgggggca ctgcctgtat gttccgctgt
      721 gggatgggcg gcgggaactg tctggcgcat ggaaatgcct tcagcaaaag ccgattttgc
      781 cgaactgctg cgcgtttcgg cgcgaatcgg ctgtctcagc tttggcgggc cggcggcgca
      841 gatcgcgctg atgcaccgcg aaatggtgga agaacgcggc tggatcagcg aggagcaata
      901 tctccacgcg ctcaacttct gccacctgct gcccggcccc gaggcccagc aactggcgat
      961 ctggatcggc tggaaacggc atggcgtgcc gggcgggctg gtggcggggc tgcttttcgt
     1021 catccccggg gcgctggtca ttctggcgct gtcggtgctg tatggcatgg cggcgcaact
     1081 ggcgtggttt gccgcgctgt ttctgggcat caaggcggcg gtgctggcga ttgtggcgca
     1141 ggcgctgctg cgccttgcca cccgcgcgct ggcaacgccg ctgcgcaagg cggcagcggc
     1201 cgcttctttc ctggcgctgt ggctgttcaa cgcgcccttt ccgctgctgg tggccgccgc
     1261 ggcgctggcc ggctggctgg ccgcgcggct gcgaccggcg ctgctgggcc tgcccgccct
     1321 gcccgctgcc agcaccgctc ccggcacgcc cccgccctgg cgcgccagcc tgcgcgcggc
     1381 gctggcgggc gcggtgctgt gggccgcgcc cctggtggcg gtgcaccggc tgctggggcc
     1441 ggggcatgtg ctgtggcgca ttggcctgtt cttttccaag ctggcggtgg tcagctttgg
     1501 cggggcctat gcggtgctgg cctatatggc gcagcaggcg gtttccgcgc agcactggct
     1561 ttcagccgcc gaaatggccg acgggctggg ccttgccgaa accacgcccg ggccgctgat
     1621 catggtcacg cagtttgtcg gctatctggc ggcctatcgg gcgccggcgc cgttcagccc
     1681 gcaggtggcc gggctgctgg gggcggggct ggtcacctgg gtcacgttca cgccgtgttt
     1741 cctgtgggtc ttcacccttg ccccctggat cgagcggctg gagcgcgcgc gccagctgca
     1801 gggcgcgctg gcgcttgtca ccgcggcgat cgtcggggtg atcggccatc tgggcagctg
     1861 gtttgcgctg caggtgctgt tccgccagct gcgcccggtg gcgctggggc cggcccacat
     1921 caccctgccg gtggccgcca gcttcgactg gcgcgccggg ctgatcgcgc tggccgccgc
     1981 ctggctggtg ctgcgcggcc gctggggcct tatcccggtg ctggcgatgg cagcggcggc
     2041 cggctggctg ctggccgccc tgcccggata agggcccccc ggcgcccggc tcaggcctcc
     2101 accagcccgg ccaccgaata ggtgatcggc tgctggcgag tggcgaggaa ttcttccaat
     2161 gtctcgccgc ggaagctggc ataaagctcg ggattggaga ggccgagcac ggccgccatc
     2221 ttctccagca cgaagaagct gaccccgcgc cgcaccatct ccagccactg gtggcccttc
     2281 accagcgcgc attcctccgc gctcagcccg aattgcgcgg ccagcccgtc gaaatcgccg
     2341 aggaagcggg cgcggtgggc cggctcgatc agccggtgga ggaaatcgtt gaggcgatag
     2401 ttggccaggc tgcgctccag cgtgaaggga tgggtgcccg gcacattctg cgcgccctcc
     2461 agctcatagc cgatatgggc gcgataggct gcattggcgg cctcatcggg cgcggtgccg
     2521 gtatcctcga acaccagcgt ggcgatgttg gtgaccgatg gcgcataggt ggcccggtgc
     2581 gtgcaccgca ccttgtcgga cagggccccg cgcatgatca gccacatgat cacctcggcc
     2641 ccttccatgc cgcccagcag cgcatattcg gccaggcgca tctccaccag cttctccggc
     2701 gcgctttcca gcaggtcgag gaattcctcg tcccaggccg ggttgaggaa gccggcgcgc
     2761 tcgccatgca cctgatgcga caggcccccg gtggccatca ccgccacccg cagatcttcc
     2821 ggatagctct cgatcgcccg gcgcaggctc ttgcccagct tgtagcagcg ccgcgcattg
     2881 gggatcggca gttgcaacac gcccacctgc agcggcacga tggcgccctg ccagggccct
     2941 tccgcatcgg ccagcatgcc cagcggcgag aggcagccat gatcgagcgg cttgccctgg
     3001 aagaagctca tgtcgaactc gtcggccacc agcgccgcgc cgatatggcg ggccagcgcc
     3061 gcatgggccc gcgccggcgc caccgcccgc gcgccgccgc cctcgtcagc ggccacatat
     3121 tggtcgtcta tgcccaggca aaaggcggaa taatggtcga agaagaagga ggtgatgtga
     3181 tcgttgtaga tggtgaagat cacgtcgatc cgctggcggc gcacccattc gcggatctcg
     3241 gcaaagccgg cgaagatcgg cgcccaggcg gggtcggcct gtttatgcgt gtccttggca
     3301 aagccgatgg tgggagaatg cgaggcgccg atgccgccca cgatgatcgc catggaagta
     3361 tcctttcccg aatgcggttg atggcttgcg gataagatgg cgccgcattg cgaacaatct
     3421 gggtgatatg taagctgcgt tctcatattg gaaacgatga ggcaccccat gcgctggcgg
     3481 ctcgatgatc tggaagtgct gtgcgccatt gccgaggcgg gcggcatcac cggcgcggcc
     3541 cgccggctgg atctgcccaa atccaccgtc agcaagctgc tcagccggct ggagcagggg
     3601 ctgggcgtgc ggctgatcga gcgcagttcg cgccgcgtgg cggtgaccag cgagggcgag
     3661 gccttcatcg cccgcgccag cctcatcctc gacatggcgc atgaggccga tgcgatgatg
     3721 caggggctgc tggccgtgcc cacaggccgc gtgacgctgg ccgcgcccgc cgccttcgcc
     3781 cgcgaagtgc tgacgccgcg cctgcatgat tttgcccggc gctatccgca gatcctgctc
     3841 gacctcgtcg tcacctcgca cccggccgag ggcattggcg agcgctgcga catggcggtg
     3901 gtggccggca tgcagcccga ttccagcctg tcgcaaaagg tgctgctggg cgggcagctg
     3961 atctggatca ccagccccgc ctatgccgcc gcccacgatc tgggcgggcc cggcgcggaa
     4021 gacctctcgc atctgatgat ctgggaaacg cgctatgccc gccatggcgt ggatgtgcat
     4081 gaagggggcc tggcccgcca cctgccgctg ccgcgcaagg ccatgcgggt gaacgatccg
     4141 atctgcgtgc gcgaggcggt gatcggcggc atgggcgtct ctttcctgcc cgagcgctat
     4201 tgcatcggcg cgctggccga tggccggctg cagcaggtgt ggccgcatct gcgcttcgac
     4261 cgggaagcgg cgcggctggc gctgctctat cccggcagca agctgctctc gccgcgcttt
     4321 cgcgccgtgg ccggctttct ggaagagatc tgcgcgcgtt gagaaggggg cggggggaaa
     4381 tccgcccccg ccccctgtct cctcagtagt tgtagcgcag cgacatgcca tagctgcgcg
     4441 gattggccgc ataggcaaac ccgcccgagc catattcctc gttatatttg agatcggtga
     4501 ggttcttgac gaagcccgcc atttcccagc gccccttgct gtcggccagg gtgaggcgca
     4561 ggttgacaag gttgagatcc ttgcgcgccg tctcgttctc gggtgaccag aactgcttgc
     4621 cgcgcagctc gtaatcgatg cgcccggtga ggttgagcgc gcccgacacc ggcatcgtgt
     4681 attgcgcgcc cacctgcacg gtcttgttag gcacgaaagg cgccttgcgg cccacatcgg
     4741 tcgggctcag cgaataggcc ttgatcgtgg catcggtgat gccggcgccc acatccaggc
     4801 ggaaaccgcc gaaatgcagc gccgcctcca gctcgccgcc attgatcgcc accttgtcga
     4861 tattgaccag cacctgggcg ctgatcgagg agacgaagac gaaatactgc acattgcgcg
     4921 cggcagtatg gaaataggtg ccatccaccg tcaggtggtt gcccagccac gaactcttga
     4981 agccggcctc gaagctgctg gtcacttcct gcttggccac atcgctcacg ccgttgaggc
     5041 cgatggtggc ggccgctgcg cccacgccgt tctggttgaa ctggccgctg cggaagccct
     5101 gaccccacga ggtgaacagg ttgatattat ccagcgcctt gtatttcagc gtgaacttgg
     5161 gctgcagctt gccgaaatgg gcattgttga catccccacc agagcccgcc gtattgtagg
     5221 gcgagacgat ctggcggcga taatcctcat catagcgcag cgccacgccc agctccagcc
     5281 ggcgggtcag atcatattcg gcattgccga aaagcgccca ggcccggtca tggttgtcat
     5341 ccgccaggaa cgacagggtg ggaatgcgcg gatcgttgaa ctgcggcacc ttctccagga
     5401 tcaccaggcc atagccaaga tcctcgctgg tgctggtgga gatgaagtgg ttgttgccca
     5461 cataataggc gcccaccatc cagcgcagcc gctggttggc gcgcgaggtc aggcggattt
     5521 cctcgctgat cgcatcggaa ctggtccatt gcgactgcac gccatcgccc accatcacgc
     5581 cattcaccat ggcatcgcgc gtggccgtat agggatactg gtcggaggca aagccttctt
     5641 ccacatgggt gaaggccgtc accgacgaca gcgtggcaaa atcgaactca taggcgctgc
     5701 gcaacgaggc cgaggcgatc gtgcgcttcg agatgccgat attgttggca tagaaggtgc
     5761 ggttcacctg attggcatcg ccccggctgt aatcgaaggg attggccgtg ttcagcgtca
     5821 cgccatcggc attgaggttg gcgggctgat aggtgaggtt cagcgcgccg gcggtggtgc
     5881 ggttgtaatt cgccttgaaa tcgaaggtca gccggtcggt cgcattatag acgaggcgcg
     5941 actggatgat cacatccttc agcggatcca ccttctggtg cagcacgata ttgtcgtaat
     6001 agccctcgcg gttctgcacg cgcacggaaa tgcggcccag cagcttgtcg cggatgacag
     6061 ggccgctcag cgtggcctcg ccgtaataat catcgccgct ggcagcgccg gcttccacat
     6121 ggccctcggt gaggttggtg ggcttcttgg tgttgatgat gatcgcgccg ccggtggcat
     6181 tgcggccata cagcgcgccc tgcggcccgc gcaccacctc gatgtcgttg atatcgagga
     6241 ggcctgcggc aaattcgcgg tcgctcgact gcagcacgcc atcgacgacg atcgccacgg
     6301 gcgcgggctg gttgcggttc tgggtgaggc cgcggatggt gatgaaagac gtgcccgggt
     6361 tctgcgaatt cacaaaagtg atgttgggcg tcatgtggat gaagtcttcc acccgcttca
     6421 caccggcatc gcgcaactgc ttttcggtga aggccgtgac cgagaccggc acctcctgca
     6481 gcttttcggc acgttgacgg gcggatacca cgatgtcacc ctgcccggtg gcggcatccg
     6541 tgccggcatt ctgggcatat gccaccgccg gaaaccatgc agcccccacc agacaattgg
     6601 cgagaaatac cgatcgcttc atgaattgtc ccctttgata ccgtccgccc tgcaacttca
     6661 tctatcttat gatgtctacg gtatacgata tctgagggct gtcaagaacc ctcttcgcag
     6721 cggaaaaggg gcccgccgcc atcaccacag cggcccgcca accgaagtgg gcgggccgcg
     6781 caaagcctcg tcattcctgg gaaatatcgg cgcaacagct agccgtccgc cagctctgcc
     6841 ggcatatcct gatagcatac cggccgccag aaacgctcga tcgccagcgt gccgacagag
     6901 gtggtgcggc tgtcgctgga ggccgggaac gggccgccat gcaccatcgc cgcggccact
     6961 tccacgccgg tgccaaagcc atcgaagagg attcgcccca ccttgcgctc cagcaccggc
     7021 atcagctggc gcaccgcggc ataatcggcc ggcacggcat gcacggccgc cgtcagctgc
     7081 ccttccagcg cgcgcagcac cgccagcagt tcggcctgat cggcgcagcg caccagcagc
     7141 gcggcggcgc caaaggtttc ctcatgcaac gccgcatcgg ccaggaaggc ctgggcatcg
     7201 gtggcaaaca gtgcggccgc gcaagtgccg ccctcgcccg cagcgccccg cgccagcagg
     7261 gccacctcag cgcgcccctc cagcagggcc agccgcgcgc cataggccgc agcgatcgcc
     7321 ggcgtcagca tggtggccgc cggcgcctgc cgcagggccg cgcaggcagc cgcctcgaaa
     7381 cggtcgaaat caggccccgc cacgcccagg atcagccccg gattggtgca gaactgcccc
     7441 gcccccagca ccagcgaggc aacgaaagcc tggccgatcg cctctgcccg cgcagccagc
     7501 gcgccgggca tcagcaccac cggattgatc gcgctcatct cggcatagac cgggatcggc
     7561 accggccgct gctgcaccag ccgcatgagc gccagcccgc ccgcgcgcga cccggtgaag
     7621 cccaccgcct gcacatggcc atctgccacc agcgcggccc ccaccgcatg gcccgcgcca
     7681 tgcagcagcg agaaaacgcc cgccggcatg ccactgcgcg ccgccgcccg ggtgatcgcc
     7741 agccccacca gctcgctggt gccggggtgg gccggatgcg ccttgacgat caccgggcag
     7801 ccggcggcca gcgcactggc cgtgtcgccc cccgccacgc tgaaggccag cgggaagttc
     7861 gaggcgccaa acaccgccac cgggcccagc ggcaccctct gcatccgcag atccggccgg
     7921 ggggcgggcg ccctgtcggg cagggcggga tcgtggcgct cgcccagccc ctcgcccttg
     7981 cgcagcatct cggcaaacag ccgcagctgg cccatcgtgc gcccgcgctc gccctccagc
     8041 cgggcgcgcg gcaggccggt ttcggccatg cagcgcacaa tcagctcatc gcccagcgcc
     8101 agaatctcct cggccaccgc ctcgagaaag gccgcccgcg cggccagcgg gtggccgcga
     8161 taggcatcgc aggcctgctc ggcggcaagg caggccgcgg ccacctgcgc cgccgatgcc
     8221 tcgcgatagg ccggcgccag cagcgcgccg ctgactgcgg cctgtgcgtg aaaagccggc
     8281 ccgctgcctt cgcaaggctg gccggcaata tacatcatgc ccgaaagcgc cattttcatc
     8341 tccactgtcc cgatcttcaa tcccgccgat cttcaatcca gcagggcgcg gatgcgcgcc
     8401 agcagcgcac catccaccgc catgccctcg cgcaggctgc gggcccgggc ggcgtggcgg
     8461 cgttctcccg gcaggcgcgc gccctgatcg cgcatgccgg caaacagcgc ctcggcctgc
     8521 gccagggccc ctgccgccgc cccgcccaga aagccggccg gatccagcgc caaaatcagc
     8581 tgcccgccca gcggcgaggc cccgcgcccg gcatcggccg ccagcgaggc ggcgctggtg
     8641 agatcgccga tcagcggccc ggccagcaat tccaccatgg ccgccagcgc cgatcccttg
     8701 tgcccgccaa acggcagcat ggccccggcc atggcggcgg cggcatcggt agtgggctgg
     8761 ccatcgccat ccagcgccca gccggcaggg atcggcaggc cagccaggcg gtgcagctcg
     8821 atctcgccgc gggccgcagc gctggtggcc agatcgaaga cgaaaggcag cgcccctgcc
     8881 cgcggccagg caaaggcgat gggattggtg ccgaacagtg gccgcgtgcc gcccgccggc
     8941 gccacccagg catggctggg cgtgcacgac agcgcgcaca gccccgcctc
//
LOCUS       DASRYE010000197        10983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_41981,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000197 DASRYE010000000
VERSION     DASRYE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41981"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFF98_10295"
     CDS             <1..306
                     /locus_tag="VFF98_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HZU64063.1"
                     /translation="QLCPGKEAASGQWTEVAPQAGAITCNIGDMLMRWSDDRLQSTLH
                     RVRMPRAGEYMGQRVSMPFFCQANRDVLIECPAGTYPPITAADYLKQRIAANFAGTR"
     gene            complement(366..1136)
                     /locus_tag="VFF98_10300"
     CDS             complement(366..1136)
                     /locus_tag="VFF98_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU64064.1"
                     /translation="MTEAIGPLSIQLWSTRGEDPLDEQFAALAAMGYTDVQPYHDQYD
                     DVPALKAALDKAGLTAVSGHFNLSMFEGDAKLVIDAARQLGMQLVVAPWLDPEDRPTE
                     AEGWKALHKRLKAMKEVIEGAGLGFAWHNHDFEFQRYADGSYGIEYLLDEDIDFAMDL
                     AWIHVGGEDPGKWLQRYAGRVPAIHVKDVAAPGQNLDQMGFADLGTGVVDWNRLWALA
                     GEAGVPLRVAEHDLPGDWRRFAGTTAAALKVLRGGGQL"
     gene            complement(1169..2518)
                     /locus_tag="VFF98_10305"
     CDS             complement(1169..2518)
                     /locus_tag="VFF98_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZU64065.1"
                     /translation="MPAPGHKAPPLAPLGLGQKLAYGCGDLSSNLVWGLMLSYLMYYY
                     TDIYVIPAATVAWLLLVPRVFDAFADPMVGYLVDRSGGRHVTRLMGLLAVPFGLATFL
                     CFLPLPLGPQGKLAWAFGSYLLLGGVYSAVNTPYGVLSNMMAIRPQERVSLNAFRLAG
                     CQLGQIIVASLTLPAIAFLGGGGSLAQQQHGITWLAALLGAGAAALWLVTWRSCRVRR
                     ALPLEVHGPVTLLRTLIGNRRWHLSNVLTYLNFTVFCSEGGLTIHYTRFVLGHPARDA
                     SLLLTLATVSAFAGVLVIPAVTRRFGIRHTYLGLLLWEMGCLAGMALAGSNFTLFATF
                     MALQYLANGPVSPLCLAMLSEAVDEGRARTGIAAAGLAFSSNTLISKVATGVAGFAIA
                     TFLAQGHYRPDLAAPSAQLVGWLKLGFMGLPAAAITIAFLLVWASRPDHHQLADTHD"
     gene            complement(2607..4289)
                     /locus_tag="VFF98_10310"
     CDS             complement(2607..4289)
                     /locus_tag="VFF98_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZU64066.1"
                     /translation="MTDLGERFDVVVVGSGAAGSFAALELAEQGLSVLVLEAGRDVGP
                     ADMLGAMGRATRGPNLAGRIKATLSGQWVQARVAFFNSRMVRFLVNDLLHPYRAAAGA
                     PFLWFRGRQVGGRLHIFGRVLHRWSDAEFAAHPGSKGEAWPIRHADLAPYYEKAERLL
                     AVQGNADGAETLPDGAFAGPGKLTLEEQGFKARVEGRWPGRRVVSWRFAPPDPTPRPT
                     ALEAALATGRVTLRADAVVCEVLTDPATGLATGVRYRDRAGRAAHVVQARAVVLGASP
                     VETVRLMLASRSARHPDGIGNSKGQLGRYFMDQPATILLAHFPGASDDPADDLPLDPV
                     YGKTGGVFLPRYPASAAASGAQLGYAYQGSIGRTPHVSPAGDRRDANFMCFSEMQPQA
                     DNRITLDPAAKDRWGVPLPVITCRLGRVERAELPAQAEDIADMVEDAGGTVTGWISPL
                     GLTERGEGVYRDLNPVSRWFMRQMLPKSFTMGAAIHETGGARMGDDPQRSVLNGWNQC
                     WDAPNLVVTDASAFVSSGTMGTTLTVMALAMRASAHLAQALRAGEPLGERAA"
     gene            complement(4396..6633)
                     /locus_tag="VFF98_10315"
     CDS             complement(4396..6633)
                     /locus_tag="VFF98_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64067.1"
                     /translation="MFADRIDNRRAHSALARAPWAAGVLRCSSALMALAALGCGSAAL
                     ADTAATATAPAPADGLGDIVVTAEHVKNNAQTTAIALSVIKGDDLKAAGIADLSALSA
                     MAPDLGFAQTEGKPMITIRGISSRDTTEVGDPAVTVSTDGFYLNRPYSLNATMYDIDR
                     VEILRGPQGTLNGRNSVGGAINIITAHPTDHYEGYASLEYGNYNTLVAQGAINIPLSD
                     AVQVRGSFYTSSHDGYRNNGVAGYGDDEDNKSGRIQIAFKPADNLHGLITAEYTKMGG
                     IGDVMQNIPFAYNSTTGALIHAMPSGINSSAFNLLTKPYLDSTEKQVRGNLVYDIAGI
                     EITALGGYDETAWHHSVDQSSQNGSSDPYQFQENQFPKTWNAELRIASKKAGPFQWQV
                     GGFFFQENSHLVSADATPLSSGYDYYFGFAYGTKTSSEAGYAQASYQLTHQLKLSAGV
                     RYTHDYKAEWGYYGNLTTGVVYAYQSGATSSSRPTFHIGLDDQLTRHNLVYAKFDTGY
                     KAGGFNFGGSSYKPESVKAFEIGSKNRFLGDSLQLNLAAFYNDYANQQVSNYAYLGNG
                     QPVQLTQNAGTSHIYGIESELIYKIPVIGTLNLTANWLHARYTNFLSVADPSDPAASG
                     NVQLAGNTPPQSPTWSLAAGLTHDWDAFGGTLTGRIQTKYQSSSNFSFYNFADTRQAP
                     YTMSDAFLTFRPGDSGWSVTAYVKNLENRTVLSDAEQSQYAAAYAYEFYPPRTYGLRI
                     QYNWK"
     gene            7480..8520
                     /locus_tag="VFF98_10320"
     CDS             7480..8520
                     /locus_tag="VFF98_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU64068.1"
                     /translation="MADTDDAPAPPLSAARLVDVAEAAGVAIATVSRALNNPGRVNAE
                     TRSKVIAAAERLGYASNMAARKLRSGQSRIIMAVMPPWGRGSVLKEILSGVDMELARS
                     RYSLIVGTLNTDRSASPRVVEMARGGLVDGLIAITNEPPADGDLAVLSARLPAVGVMI
                     DLSEHGIPSVITDEAAAIAELTASMVAEGRSYLAYLSGPGSAFPARGPNYHEEQRYAG
                     FLQAAQRVRHCRIEGDFTHAAGERAAQWFLAQEDPPNGIVCANDHMAVALIDALRRRG
                     VRIPEQVAITGFDDEDIARYCYPSLTTVRPALKEMGATSVRLLMQLLSGAISPPIPPR
                     VISATIVRRESF"
     gene            complement(8517..9467)
                     /locus_tag="VFF98_10325"
     CDS             complement(8517..9467)
                     /locus_tag="VFF98_10325"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZU64069.1"
                     /translation="MTQIPPQGSALLPLIDLAGLLDGSPDGAARVAEAIGHACRTTGF
                     FYVVGHGIEPRLLEAVFALQRRFFALPLAAKAALAIEQAGNNRGYVGMESENLDPDAP
                     HDVKEAFNIGRESTPGDAAAPTPWPDLPGFRAAMSQLYAALRQLSCDLHRAFALDLGQ
                     AAEYFAPLVDRPAATLRLLHYPPLGQAADGRLGAGAHCDYGNITLLAQDDVGGLEVQT
                     RDGAWIAAAPVPGAFLCNIGDCLMRWSNDIYVSTPHRVISPAARDRYSLAFFFDPNPD
                     AWISCLPSCVSAGRPAAHAPIRFDAYLAEKLASTYRRAAD"
     gene            complement(9566..>10983)
                     /locus_tag="VFF98_10330"
     CDS             complement(9566..>10983)
                     /locus_tag="VFF98_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64070.1"
                     /translation="SNNYSKLDPASISQVQLNRRTVLSSSYDFGNVETNTGGFSLAHA
                     VGSGKLKFEGFIDDLNADTYNGYGFAKRLRDRAEELRLTLNGKLDLGPQISGTYAAGA
                     NYRYYEAHEGYAYALGYLILDRQDLTVGATPDSIVNPLYVSGGAFDQVYNSTVDEMGT
                     FFDGSLKLFGLLRLTGGGRYDAYKVAASNTGLLDYSAQLNKVYRASRGVFSWSGSVSV
                     ETPWGIAPYFTRSRAYALETQQGGAVAAGYVAYNEWLSPTDLTEGGVKGSLFHNKLFF
                     AFSAYHQKRMQTQLLSNSFTGVVTNGQEAELRWAVNRNIGISATATHQKSYVHGCSYL
                     FATPSELGLANVDGWGEVYEVSTCSYAGYQSGYVDHTQPRYVLGLFVNYESGHGFGAT
                     LGGNWVDKTSGQLPGAVVFPDYYLLRGSVHYDFGRYRLSAYVNNILDTRYYIPQRSTE
                     TEGTAMPGEGRTAMLKITVHL"
BASE COUNT         1978 a         3601 c         3660 g         1744 t
ORIGIN      
        1 caactgtgtc ccggcaagga agcggccagc ggccagtgga ccgaagtggc gccacaggcc
       61 ggcgccatca cctgcaacat cggcgatatg ctgatgcgct ggagcgatga ccgcctgcaa
      121 tccaccctgc accgggtgcg catgccgcgc gcgggcgaat atatgggcca gcgcgtgtcg
      181 atgccgttct tctgccaggc caaccgcgat gtgctgatcg aatgcccggc cggcacctat
      241 ccgccgatca ccgcggcaga ctatctgaag cagcgcatcg ccgccaattt cgccggcacg
      301 cgctgaggcg attggcgcga ggccaagaag ggggcgccag ccagcgcccc ctttgtcctt
      361 tgcgcttaca gctgcccgcc gccgcgcagc accttcagcg cggcggccgt ggtgccggca
      421 aagcggcgcc agtcgccggg caggtcatgc tcggccacgc gcagcggcac gccggcctcg
      481 ccggccagcg cccacaggcg gttccagtcc accaccccgg tgcccagatc ggcaaagccc
      541 atctggtcga ggttctggcc gggggcggcc acatccttca catggatcgc cggcacgcgc
      601 ccggcatagc gctgcaacca cttgcccgga tcctcgccgc caacatggat ccaggcgagg
      661 tccatcgcaa agtcgatatc ctcgtccagc agatattcga tgccatagga gccatcggca
      721 tagcgctgga attcgaaatc atggttgtgc caggcaaagc caaggccggc cccctcgatc
      781 acctccttca tcgccttcag ccgcttgtgc agcgccttcc agccctcggc ctcggtgggg
      841 cggtcttccg ggtccagcca gggagccaca accagctgca tgcccagctg gcgggcggca
      901 tcgatcacca gcttggcatc gccctcgaac atcgagaggt tgaaatggcc gctgaccgcc
      961 gtcagcccgg ccttgtcgag cgcggccttc agcgcgggca catcgtcata ctggtcgtga
     1021 tagggctgca catcggtgta gcccatggcg gcaagggcgg cgaactgctc gtcgagcggg
     1081 tcttcgcccc tggtcgacca cagctggatg gaaagggggc cgatggcctc ggtcatatgc
     1141 gcttgtcctc aggtctgtcg ggaaagggtc agtcgtgggt gtcggccagc tggtggtgat
     1201 cggggcgcga ggcccacacc agcaggaagg cgatggtgat ggcagcagcc ggcaggccca
     1261 tgaagcccag cttcagccaa cccaccagct gggccgaagg ggcggccaga tcggggcgat
     1321 aatggccctg cgccaggaaa gtggcgatgg caaagccggc aacgccagtg gccaccttgg
     1381 aaatcagcgt gttgctggaa aaggccaggc ccgccgcggc aatgccggtg cgcgcgcgcc
     1441 cctcgtccac cgcttccgac agcatcgcca ggcacagcgg cgagaccggg ccattggcca
     1501 gatattgcag cgccatgaaa gtggcgaaca gggtgaaatt cgaccccgcc agcgccatgc
     1561 cggccaggca gcccatttcc cacagcaaca ggcccagata ggtgtggcgg atgccaaagc
     1621 ggcgagtgac ggcggggatc accagcaccc ctgcaaaggc cgacacggtg gccagcgtca
     1681 acagcagcga ggcatcgcgc gcggggtggc ccagcacgaa gcgggtgtag tggatcgtca
     1741 gcccgccctc ggagcagaag acggtgaagt tgagataggt gagcacattg ctgagatgcc
     1801 agcggcggtt gccgatcagc gtgcgcagca gggtgaccgg cccgtgcact tccagcggca
     1861 gcgcccggcg cacgcggcag ctgcgccacg tcaccagcca cagcgccgcc gcccccgccc
     1921 ccagcagcgc cgccagccag gtgatgccat gctgctgctg ggcaaggctg ccgccgccgc
     1981 ccagaaaggc gatcgccggc agggtgaggc tggcaacgat aatctgcccc agctggcagc
     2041 cggccaggcg gaaggcattg aggctgaccc gttcctgcgg gcggatcgcc atcatgttgc
     2101 tgagcacccc ataaggcgtg ttgaccgccg aatagacccc gcccagcagc agataggaac
     2161 caaacgccca ggccagcttg ccctgcggcc ccagcggcag cggcaggaag cacaggaaag
     2221 tggccaggcc aaacggcacc gccagcaggc ccatcagccg cgtcacatgc cgcccgcccg
     2281 agcgatccac cagatagccc accatcggat cggcaaaggc atcgaacacc cgcggcacca
     2341 gcagcagcca ggccaccgtt gccgccggga tcacatagat gtcggtgtag taatacatca
     2401 ggtaggagag catcagcccc cacaccagat tgctggagag gtctccgcag ccataggcca
     2461 gcttctgccc cagccccagc ggcgccaggg gcggtgcctt gtggccgggg gcaggcatgg
     2521 cggcatcttg caccagcgtc gatgtcatag gtagcgcttc ccgatggggc cggccagggc
     2581 gggcggccag aggcagatgc tagaactcag gcggcgcgtt cgccaagcgg ttcgcccgcc
     2641 cgcaaggcct gcgccagatg ggccgaggcg cgcatggcca gcgccatcac cgtcagcgtg
     2701 gtgcccatgg tgccgctgga gacaaaggcg ctggcatcgg tgacgaccag gttgggcgca
     2761 tcccagcact ggttccagcc gttgagcacc gagcgctgcg gatcatcgcc catgcgcgcg
     2821 ccgcccgtct cgtggatggc cgcgcccatg gtgaagcttt tgggcagcat ctgccgcatg
     2881 aaccagcgcg agaccgggtt gagatcgcgg tagacgccct cgccccgctc ggtcaggccc
     2941 agcggcgaga tccagccggt cacggtgccg ccggcatctt ccaccatgtc ggcgatatcc
     3001 tcggcctggg cgggcagttc ggcccgctcc acccggccca gccggcaggt gatcaccggc
     3061 agcggcacgc cccagcggtc cttcgcggca ggatcgaggg tgatgcggtt atccgcctgc
     3121 ggctgcattt cgctaaagca catgaaattg gcatcgcgcc ggtcgcccgc cgggctcaca
     3181 tggggcgtgc gcccgatcga gccctgatag gcatagccca gctgcgcgcc ggatgcggcc
     3241 gcgctcgccg gatagcgcgg caggaacaca cccccggtct tgccatagac cggatcgagc
     3301 ggcagatcgt cggccggatc atcgctggcg ccggggaaat gggcgagcag gatggtggcc
     3361 ggctgatcca tgaaatagcg gcccagttgg cccttgctgt tgccgatgcc atcgggatgg
     3421 cgggccgagc gcgaggccag catcagccgc accgtttcca caggcgaggc acccagcacc
     3481 accgcgcggg cctgcaccac atgcgcggcc cggccggcgc gatcgcgata gcgcacgccg
     3541 gtggcaaggc cggtggccgg gtctgtcagc acctcgcaca ccaccgcatc ggcgcgcagc
     3601 gttacgcggc cggtggccag cgccgcttcc agcgccgtgg gccgcggcgt gggatcaggc
     3661 ggggcaaagc gccacgaaac cacccgccgc cccggccagc gcccttccac ccgggccttg
     3721 aagccctgct cctccagcgt cagcttaccg gggccggcaa aggcgccatc gggcagggtt
     3781 tcggccccgt cggcattgcc ctgcacggcc agcagccgct cggctttttc gtagtagggg
     3841 gccagatcgg cgtggcggat cggccaggcc tcgcccttgc tgccgggatg ggcggcaaat
     3901 tccgcatccg accaacggtg cagcacgcgg ccgaagatat gcagccgccc gcccacctgc
     3961 cgcccgcgaa accacaggaa cggcgcgccg gccgccgcgc gatagggatg cagcagatca
     4021 ttcaccagaa agcgcaccat gcggctgttg aagaaggcca cccgcgcctg cacccattgc
     4081 ccgctgagcg tggccttgat gcggccggcc aggttgggcc cgcgcgtggc ccgccccatg
     4141 gcgcccagca tgtcggccgg gcccacatcg cggcccgctt ccagcaccag caccgaaagg
     4201 ccctgttcgg ccagttccag cgcggcaaag ctgcccgccg cgccagagcc caccaccacc
     4261 acatcgaaac gctcgcccaa gtcggtcata tctgtcctgt tgctttcgtc tttgccggcc
     4321 tgcccgcgcc aaaaggggtg ccgccggccc cttgcgcgcc cgaacgcacg caagcagccg
     4381 gcggcggggg aaggcttatt tccagttgta ttgaatgcgc aggccatagg tgcgcggcgg
     4441 atagaactca taggcatagg ccgcggcata ctggctctgc tcggcatcgc tgagaacggt
     4501 gcggttctcc agattcttca cataggccgt caccgaccag ccgctgtcgc ccgggcggaa
     4561 ggtgaggaag gcatccgaca tggtgtaggg cgcctgccgg gtgtcggcga aattgtagaa
     4621 ggagaagttg gaggaggact ggtacttcgt ctggatgcgg ccggtcagcg tgccgccaaa
     4681 ggcgtcccag tcatgcgtca acccggcggc caggctccag gtgggcgatt gcggcggcgt
     4741 gttgccggcc agctgcacat tgccgctggc cgcaggatcg ctgggatcgg ccaccgagag
     4801 gaagttagta taacgcgcat gcagccagtt ggcggtgagg ttcagcgtgc cgatcaccgg
     4861 gatcttgtag atcagctcgc tctcgatgcc gtagatgtgg ctggtgccgg cgttctgcgt
     4921 cagctgcacc ggctggccat tgcccagata ggcgtagttc gagacctgct ggttggcata
     4981 atcattgtag aaggcggcca ggttcagctg caggctgtcg cccaggaagc ggttcttgct
     5041 gccgatctcg aaggccttca cgctttccgg cttgtaggag gagccaccga agttgaagcc
     5101 gcccgccttg tagccggtgt cgaacttggc atagacgagg ttgtggcggg tcagctgatc
     5161 gtccagcccg atatggaaag tgggccgcga ggaggatgtg gcgcccgact gataggcata
     5221 gacaacgccg gtggtgagat tgccgtaata gccccattcg gccttgtagt catgcgtgta
     5281 gcgcacgccg gccgacagtt tcagctgatg cgtcagctga taggaggcct gggcatagcc
     5341 ggcttccgac gaggtcttgg tgccataggc aaagccgaag taatagtcat agcccgagga
     5401 cagcggcgtc gcatcggcgc tgacgaggtg cgaattctcc tggaagaaga agccgccgac
     5461 ctgccactgg aagggccccg ccttcttcga ggcgatgcgc agttccgcgt tccaggtctt
     5521 ggggaactgg ttttcctgga actggtaggg gtcgctcgaa ccattctgcg acgactgatc
     5581 gaccgaatgg tgccaggcgg tttcgtcata gccgcccagc gcggtgatct cgatgccggc
     5641 gatatcgtag acaaggttgc cgcgcacctg cttttcggtg ctgtcgagat agggcttggt
     5701 cagcaggttg aaggcgctgg aattgatgcc cgagggcatg gcgtggatca gcgcgccggt
     5761 ggtgctgtta taggcaaagg ggatgttctg catgacatcg ccgatgccgc ccatcttggt
     5821 gtattcggcg gtgatcaggc cgtgcagatt atccgccggc ttgaaggcga tctggatgcg
     5881 gccagacttg ttgtcctcgt catcgccata accggccacg ccattgttgc gatagccatc
     5941 atgcgaggaa gtgtagaacg agccgcgcac ctgcaccgca tcgctgagcg ggatgttgat
     6001 ggcgccctgc gccaccagcg tgttgtaatt gccatattcg aggctggcat agccttcgta
     6061 atggtcggtg gggtgggcgg tgatgatgtt gatcgcgccg cccaccgaat tgcgcccgtt
     6121 gagcgtgccc tgcggcccgc gcaggatttc cacgcggtcg atgtcataca tcgtggcgtt
     6181 cagcgaatag gggcggttga gatagaagcc atcggtgctg acggtgaccg ccggatcgcc
     6241 cacttcggtg gtgtcgcggc tggaaatgcc gcggatggtg atcatcggct tgccctcggt
     6301 ctgggcaaag cccagatcgg gcgccatcgc cgacagggcg gacagatcgg caatgccggc
     6361 cgccttcaga tcatcgccct tgatgaccga cagcgcgatg gcggtggtct gggcgttgtt
     6421 cttcacatgc tcggcggtga cgacgatatc gcccagcccg tcggcgggcg caggcgcggt
     6481 ggcggtggct gcagtgtcgg ccagggcggc agagccgcag cccagcgccg ccagcgccat
     6541 cagcgcgctc gaacagcgca gaacgcccgc cgcccacggc gcacgggcca gggcggaatg
     6601 ggcacgtcga ttgtcaatcc tgtcagcaaa catggcgaga cccccattgg gcaaacggga
     6661 tatttgccca tcttgtttgg tgtgttcctg aagcgtcttt gttattggcc gggcgatccg
     6721 gcaggcttgg gttatgcggc aggccagtca ggcgggcggt gaggcccggc cggcgccccg
     6781 gcacttcccg ccgccgctgg cctgtggggc caggcggaag gaaagcgttg cgcatgatcg
     6841 atccgctgcg cctccaggga atcgcggccc gactggtata tttcggttaa attcagcaga
     6901 aatcggcgaa attcagtgac atttcgcgga tggcaaacgt tatccgcaaa gccaagctat
     6961 cgccattgcc gcgcccttgc catgacaaat ttgaggtatt agcgttcaac taattagaac
     7021 gcaaaaagcc ctcaaatgcc gtaattggga cgggcgcgcg gaattttccc ggaaatcccg
     7081 caggatggcc gcgcccgccg cggcgaaagg cagcaccacc accttgccca gcccaacgaa
     7141 aaggccttcc gtgtagaaga aattgaggca accgtgcact cacccacccg ctgccggaaa
     7201 atcaggctgg aatcgttttc cacgcccatt cccgtcgccc tcgcttgccg gcaggggccc
     7261 ttgcgcatgg cgtttaccac gcccgccagc gccgttttcc agcaaagcgg cttgaccggg
     7321 ccccgccatt ggcgcaggca cccggcaggg gcgcgccgat ctgcaacatg gctgccatct
     7381 gcgcctgccc tcgcccgcga tctgccgccc ggctgccatg cgctctccag cagagcgcaa
     7441 ggggccccgc ccagggcgcc ggccagttgg agacgagatt tggcagacac agacgacgcc
     7501 cccgccccgc ccctgtcggc cgcccggctg gtggatgtgg ccgaagcggc cggcgttgcc
     7561 atcgccaccg tcagccgcgc gctcaacaat cccggccggg tcaacgccga aacccgcagc
     7621 aaggtgatcg ccgcggccga acggctgggc tatgcctcga acatggcggc gcgcaagctg
     7681 cgctcgggcc agtcgcgcat catcatggcg gtgatgccgc cctggggccg cggcagcgtg
     7741 ctgaaggaga tcctcagcgg ggtcgacatg gagctggccc gctcgcgcta cagcctgatc
     7801 gtcggcacgc tgaacaccga tcgcagcgcc tcgccgcggg tggtggaaat ggcgcgcggc
     7861 gggctggtcg acgggctgat cgccatcacc aacgagccgc ccgccgatgg cgatctggcg
     7921 gtgctgtcag cccggctgcc ggcggtgggg gtgatgatcg acctcagcga gcacggcatt
     7981 cccagcgtca tcaccgacga ggcggccgcg atcgccgaac tcaccgcctc gatggtggcc
     8041 gaggggcgct cttatctcgc ctatctatcg ggcccgggca gcgcctttcc cgcccgcggc
     8101 cccaactatc acgaagagca gcgctatgcc ggcttcctgc aggccgcaca gcgcgtgcgc
     8161 cactgccgga tcgagggcga tttcacccat gccgcgggcg agcgcgccgc ccaatggttc
     8221 ctggcccagg aagacccgcc caatggcatc gtctgcgcca atgatcacat ggccgtggcg
     8281 ctgatcgacg ccctgcgccg gcgcggcgtg cgcatccccg aacaggtggc gatcaccggc
     8341 ttcgacgatg aagacatcgc ccgctactgc tacccctcgc tgacgacggt gcgcccggcg
     8401 ctgaaggaaa tgggcgccac cagcgtgcgc ctgctgatgc aattgctgag cggggcgatc
     8461 agcccgccga tcccgccgcg agtgatttcc gccactatcg tgcggcgcga atccttttaa
     8521 tccgccgccc ggcgataggt ggaagccagc ttttcggcca gataggcatc gaaacggatc
     8581 ggcgcatggg ccgccgggcg cccggcgctg acgcagctgg gcaggcagct gatccaggca
     8641 tcggggttgg gatcgaagaa aaaggccagc gaatagcgat cgcgcgcggc cgggctgatc
     8701 acccggtgcg gggtggagac atagatgtcg ttgctccagc gcatcaggca atcgccgatg
     8761 ttgcacagga aagcgccggg cacaggcgcg gcggcgatcc aggcgccatc acgcgtctgc
     8821 acttccagcc cgcccacatc atcctgggcc agcagggtga tattgccata atcgcaatgg
     8881 gcgccggccc ccagccggcc atcggccgcc tgccccagcg gcggataatg cagcagccgc
     8941 aaggtggccg ccggccgatc caccagcggg gcgaaatact ccgccgcctg ccccagatcg
     9001 agcgcgaagg cccggtgcag atcgcaggac agctggcgca gcgccgcata aagctggctc
     9061 atcgccgcgc gaaagcccgg cagatccggc cagggcgtgg gcgcggcggc atcgcccggg
     9121 gtgctttccc ggccgatgtt gaaggcctcc ttcacatcat gcggggcatc gggatcgagg
     9181 ttctcgcttt ccatgcccac atagccgcgg ttgttgccgg cctgctctat cgccagcgcc
     9241 gccttggccg ccagcggcag ggcgaagaag cggcgctgca gggcaaacac cgcctccagc
     9301 aggcgcggct ctatgccatg gcccaccaca tagaagaaac cggtggtgcg gcaggcatgg
     9361 ccaatggcct ctgccacgcg ggcggcgcca tcggggctgc catccagcag gccggcaagg
     9421 tcgatcagcg gcagcagggc gctgccttgg ggggggatct gcgtcatgag gggatatccg
     9481 ggttcggccc ctgcccgaca tggctgacag gcagggctga caggcagggc tggcgggcag
     9541 gggcgcgggg ggaatacaaa cgggatcaga gatgcacggt gatcttcagc atcgccgtgc
     9601 gcccttcgcc gggcatggcg gtgccttccg tctcggtgct gcgctggggg atatagtagc
     9661 gggtatcgag gatgttgttc acataggccg agaggcgata gcggccgaaa tcataatgca
     9721 ccgagccgcg cagcaggtaa taatcgggga acacaaccgc gcccggcagc tggccgctgg
     9781 tcttgtccac ccagttgccg cccagcgtgg cgccaaagcc atggccgctc tcgtaattga
     9841 cgaaaagccc cagcacatag cgcggctggg tatgatcgac ataaccgctc tgatagcccg
     9901 cataggagca ggtggagacc tcatagacct cgccccagcc atcgacattg gccagcccca
     9961 gttccgatgg cgtggcaaac aggtaggaac agccatgcac atagctcttc tgatgcgtgg
    10021 cggtggcgga aatgccgata ttgcggttca ccgcccagcg cagctctgcc tcctgcccgt
    10081 tggtgacaac gccggtgaag ctgttcgaaa gcagctgtgt ctgcatgcgc ttctggtgat
    10141 aggctgaaaa ggcgaagaac agcttgttgt ggaacaaaga gcccttcacc ccgccctcgg
    10201 taagatcggt aggcgagagc cattcgttat aggcgacata gccggccgcc accgccccgc
    10261 cctgctgggt ttccagcgca taggcgcggc tgcgggtgaa atagggggca atcccccagg
    10321 gcgtttccac cgacacgctg cccgaccagc tgaacacgcc ccggctggcc cgatagacct
    10381 tgttaagctg ggcggaataa tcgagcaggc cggtgttgct ggccgccacc ttataggcat
    10441 cgtagcgccc gccgccggtc agccgcagca ggccgaacag cttgagcgag ccatcgaaga
    10501 aggtgcccat ctcgtccacg gtggaattat agacctgatc gaaagccccg cccgagacat
    10561 agagcgggtt gacgatgctg tcgggcgtgg cgccaacggt gagatcctgc cgatcgagga
    10621 tgagatagcc cagcgcatag gcatagcctt catgcgcctc gtaatagcga tagttggcgc
    10681 cggcggcata ggtgccggaa atctgcgggc ccagatccag cttgccgttc agcgtcagcc
    10741 gcagctcctc ggcccggtcg cgcaggcgct tggcaaagcc atagccattg taggtatcgg
    10801 cgttgagatc atcgatgaac ccctcgaact tcagcttgcc cgaacccacc gcatgggcga
    10861 ggctaaagcc gccggtgttc gtctcgacat tgccgaaatc gtaggaggag gagagcaccg
    10921 tgcgccggtt gagctgcacc tggctgatcg aggccggatc cagcttcgaa taattattcg
    10981 agg
//
LOCUS       DASRYE010000198         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_26701,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000198 DASRYE010000000
VERSION     DASRYE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26701"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFF98_10335"
     CDS             complement(<1..767)
                     /locus_tag="VFF98_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HZU64071.1"
                     /translation="MSISDARLAQLSHRFAELEARLASGTLEGADFVAASRDYAELEP
                     VAKAARQVAGMRAEQASLATLDDPEMRELAEEELARLKAELPAAERALAIAMLPRDTA
                     DARPAMLEIRAGTGGDEAALFAADLFRMYERYAATQGWRVEMISANASELGGFKEVVA
                     HVSGQGVFARLKFESGVHRVQRVPVTESGGRIHTSAATVAILPEPDEVDVHIEDKDLK
                     IDIYRASGAGGQHVNTTDSAVRITHLPSGLVVICQDER"
     gene            complement(764..2008)
                     /gene="hisS"
                     /locus_tag="VFF98_10340"
     CDS             complement(764..2008)
                     /gene="hisS"
                     /locus_tag="VFF98_10340"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZU64072.1"
                     /translation="MQTGKIETPQAIRGTQDIFGADAEAFARVVEVFERVRRLYRFRR
                     VEMPVFEKTAVFSRSLGETTDVVSKEMYSFEDRGGESLTLRPEFTAGIARAYLTNGWQ
                     QFAPVKIATHGPLFRYERPQKGRYRQFHQIDAEVIGAAEPTADVELLVMADQLLKELG
                     IADGVTLTLNTLGDGASREAWRAALIDYFRAVKGDLSEDSQERLEKNPLRILDSKDPR
                     DKAFLPDAPRIDDFLSDEAQDFFARVTGGLDAAGVAWVRNPALVRGLDYYRHTAFEFV
                     TDRLGAQGTVLGGGRYDGLMESLGGTATPAVGWAAGVERLAMLVADAGQLAEAPLDVV
                     LAVEDDSLVTLAIAASAALRRAGLASEMVASGSPRKRFDKAAKIPASVLVGLSLRDGA
                     VVFNYRGADTSAVQALLDPLRP"
BASE COUNT          333 a          771 c          687 g          332 t
ORIGIN      
        1 cgctcgtcct ggcagatcac caccagcccc gagggcagat gggtgatgcg cacggccgaa
       61 tcggtggtgt tcacatgctg gccacccgcg ccgctggccc ggtagatgtc gatcttgagg
      121 tccttgtcct cgatatgcac atccacctcg tcgggctcgg gcaggatggc cacggtggca
      181 gcgctggtgt ggatgcgccc gccgctttcc gtcaccggca cgcgctgcac ccggtgcacg
      241 ccgctctcga acttcagccg ggcaaacacc ccctgccccg aaacatgcgc caccacttcc
      301 ttgaagccac ccagctcgct ggcattggcg ctgatcatct ccacccgcca gccttgcgtg
      361 gcggcatagc gttcatacat gcggaagaga tcggcggcga agagggcggc ctcatcgccg
      421 ccggtgccgg cgcggatttc cagcatggcg gggcgggcat cggcggtgtc gcgcggcagc
      481 atggcgatcg ccagcgcccg ctcggccgcc ggcagctcgg ccttcagccg ggccagctcc
      541 tcctcggcca gctcgcgcat ttccggatcg tcgagcgtgg caaggctggc ctgttcggcg
      601 cgcatcccgg ccacctggcg ggccgccttg gccaccggct ccagctcggc ataatcgcgg
      661 ctggcggcaa cgaaatcggc cccctccagc gtgccggagg caaggcgggc ctccagctcg
      721 gcaaagcggt gggaaagctg cgcgaggcgc gcgtcggaaa tgctcacggg cgcaaggggt
      781 cgagcagcgc ctgcaccgcc gatgtgtcgg ccccgcgata gttgaacacc accgccccat
      841 cgcgcagcga caggcccacc agcacgctgg ccgggatctt ggcggccttg tcgaaacgct
      901 tgcgcgggct gccgctggcc accatctcgc tggcaagccc cgcccggcgc agcgcggcgc
      961 tggccgcgat ggccagggtg acgaggctgt cgtcctccac cgccagcacc acatccaaag
     1021 gcgcctcggc cagctgcccg gcatcggcca ccagcatcgc cagccgctcc acgccagccg
     1081 cccagcccac cgccggggtg gccgtgccgc ccaggctttc catcagcccg tcatagcggc
     1141 cgccacccag cacggtgccc tgcgcaccca gccggtcggt cacgaattca aaggcggtgt
     1201 ggcggtaata gtccagcccg cgcaccagcg ccgggttgcg cacccaggca acgccggccg
     1261 catccagccc gccggttacc ctggcgaaga aatcctgcgc ctcatcgctg aggaaatcgt
     1321 cgatcctcgg ggcatcgggc aggaaggcct tgtcgcgcgg gtctttggaa tcgaggatgc
     1381 gcagcgggtt cttctccagc cgctcctgcg aatcctcgga aagatcgccc ttcaccgcgc
     1441 ggaaataatc gatcagcgct gcccgccatg cctcgcggct ggcgccatcg cccagcgtgt
     1501 tcagcgtcag cgtcacgcca tcggcaatgc ccagctcctt cagcagctgg tcggccatca
     1561 ccagcagctc cacatcggcg gtgggctcgg ccgcgccgat cacctcggcg tcgatctggt
     1621 ggaactggcg atagcgcccc ttctgcggcc gttcatagcg gaacagcggg ccatgggtgg
     1681 caatcttcac cggcgcgaac tgctgccagc cattggtgag ataggcccgc gcgatgccgg
     1741 cggtgaattc gggccgcagg gtcagcgatt cgccgccgcg atcctcgaag gaatacatct
     1801 ccttcgagac cacatcggtc gtctcgccca ggctgcgcga gaacaccgcg gtcttctcga
     1861 acaccggcat ttccacgcgg cggaagcggt aaagccggcg cacgcgctcg aacacttcca
     1921 ccacgcgggc gaaagcctcg gcatcggcgc cgaaaatgtc ctgggtgccg cgaatggcct
     1981 gcggggtctc gatcttgcct gtttgcatgg gcaagcgctc tagctgcgcg gcgcgccaag
     2041 gtgaagcccc ttcctgcgcg ccgggcggtc cactttcgcg ggcgattccg ggccaagagg
     2101 ctggttgcag acagggtttt ttc
//
LOCUS       DASRYE010000199         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_60634,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000199 DASRYE010000000
VERSION     DASRYE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60634"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFF98_10345"
     CDS             <1..402
                     /locus_tag="VFF98_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZU64073.1"
                     /translation="PPPPPPPPPPPPPPAPVCNKGPYIVFFDFDKSDITPEAGTILDS
                     AVQAYANCQTIPIMLAGYTDLAGTQKYNLGLSARRNASVRAYLTSHGIADSVISSQAF
                     GKLHPRVPTADGVRELQNRRVEITYGPGSGN"
     gene            complement(582..1280)
                     /gene="queC"
                     /locus_tag="VFF98_10350"
     CDS             complement(582..1280)
                     /gene="queC"
                     /locus_tag="VFF98_10350"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZU64074.1"
                     /translation="MATLASGEGRLAVVLLSGGLDSMVSAGLAREAGYRICALTIDYN
                     QRHRRELDAAQAVARELGVARHVVLPLDLRQFGGSALTADIDVPKEGVGEEIPVTYVP
                     ARNLVFLSLTLAWAESLGARDVFIGVNALDYSGYPDCRPEFISGFEHLARLATKAGAE
                     GDDFRIHAPLQYLGKAEIVSEAFRLGLDPAISWSCYDPQPDGTACGKCDSCRLRLEGF
                     AKAGRVDGVPYAAL"
     gene            complement(1327..2229)
                     /locus_tag="VFF98_10355"
     CDS             complement(1327..2229)
                     /locus_tag="VFF98_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZU64075.1"
                     /translation="MSADQPAEPHSPEGLLAFTLADRNARGRVVRLGPVLREILSAHA
                     YPPVLARLLAEALTVTALMGSLLKDDDGQLTMQAQSTEGPISLLVCDYRQGELRGYVQ
                     HDEEALAALGPAPAIADLFGSGYLAITFDLAVSGQRYQGIVPLEGASLAAACESYFVQ
                     SEQVPTLIRVEVEQGEEGYVAGGLLMQHLPDGEEGRERLHVRLDHPEWQHVAIMAGSI
                     RGAELVDQGLPLDWLVWRLFHEEREVRVLPQQALHRGCRCTLAHYEAILSQFPEAERA
                     EMRNEAGEIVVDCAFCSRIFPIKV"
     gene            complement(2226..3152)
                     /gene="argF"
                     /locus_tag="VFF98_10360"
     CDS             complement(2226..3152)
                     /gene="argF"
                     /locus_tag="VFF98_10360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZU64076.1"
                     /translation="MSRHFLNLADAGPAALRAMLADAGARKAARASWPKGRADADAPL
                     AGHVLAMIFEKNSTRTRVSFDMAMRQLGGSAIVMESGSMQLGRGETIADTARVLSRMV
                     DAIMIRTNDHAKIEELARYASVPVINGLTDLSHPCQIMADLLTLAERGVALEGMEMAW
                     LGDGNNVLHSLIEAAGLLGFHIRVGGPAGYEPDGAFVAAAQAAGSRITFTQDPKAAVA
                     GAQVVVTDTWVSMGQAGGEAHVAAMQPFQVNEELMRAAAADAVFLHCLPAHRGEEVTD
                     AVIDGPHSAVWDEAENRIHAQKAVLRWAFGQL"
     gene            complement(3152..4339)
                     /locus_tag="VFF98_10365"
     CDS             complement(3152..4339)
                     /locus_tag="VFF98_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZU64077.1"
                     /translation="MSITPLMPVYPRCGFRPVRGEHCHLIGEDGQRYLDFASGIAVNI
                     LGHSHQGLIGAIQRQAATLMHVSNLYGSPQGEHLAQRLVDLTFADTVFFTNSGAEAVE
                     CAIKTARAYHQHVGNLDKYELITFKEAFHGRTMGTISASNQEKMHKGFLPMLPGFKYV
                     DFGDLEAVKAAIGPNTGGIMIEPIQGEGGIKVPSAEFLQGLRALCDEHDLMLVFDEVQ
                     CGVARSGTFYAYEQFGVVPDILATAKGIGGGFPLGACLAREKAARGMVAGTHGSTYGG
                     NPLAMAAGEAVLDAVANEDFLAHVRAMGSLLTSRLEQFIGNYPDLFEEVRGRGLMLGL
                     KLKKEPRPFVAHLRDKHQLLTVSAGDSTMRILPPLVIDESHIDEFMERLSAGAASYMA
                     EAA"
     gene            complement(4512..5276)
                     /locus_tag="VFF98_10370"
     CDS             complement(4512..5276)
                     /locus_tag="VFF98_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZU64078.1"
                     /translation="MGFDRGRRGRGRDKRDGFGEDSFDPFMGGGDRFGGHDRFGGDRG
                     GDRGGFGGGPRGGGGGFGGGPRGGGFGGGGGGGFGGPRGGGGGGGGMPAQVVGQSKGT
                     VKFFNGQKGFGFIQREDGGEDVFVHISAVERAGLEGLAEGQGLEFTLVDRGGKISASD
                     LVVMGDVIPVAKREPAPQRQLTGEKATGTVKFFNTMKGFGFITRDDGQPDAFVHISAV
                     ERSGLRELNEGDRLEFDIEVDRRGKYSAVNLVPRQG"
     gene            5626..6057
                     /locus_tag="VFF98_10375"
     CDS             5626..6057
                     /locus_tag="VFF98_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HZU64079.1"
                     /translation="MFRKLSEAVWVSPQITTEDVAAAAAQGIGLIINNRPEGESDDQT
                     PGDAIADSAAALGLAYVAIPVTHAGFSEGQVQQMAQALAEAKGGILAYCRSGTRSTLL
                     WALAQAATGTHPDDIARAAALAGYDIGPVRALVDMLAAGQN"
     gene            complement(6261..>6801)
                     /locus_tag="VFF98_10380"
     CDS             complement(6261..>6801)
                     /locus_tag="VFF98_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HZU64080.1"
                     /translation="ATAAAAVSWALCDQIFHGKPSMLGAASGAVAGLVAVTPASGYAG
                     PMGSVVLGLLVSPLCFAFVTWVKNIFKYDDTLDVFGVHCVGGIFGALMTGILDAPSLG
                     GVGITDYSKFPAATGSYDMLGQLWIQAQGVGITLLWSGIGSLILYVIIDKIFGLRPSE
                     EAEREGLDITEHGERAYNY"
BASE COUNT         1248 a         2369 c         2102 g         1082 t
ORIGIN      
        1 cctcccccgc cgcctccgcc gcctccgccg ccgcctcccc cggctccggt gtgcaacaag
       61 ggcccctaca tcgtgttctt cgacttcgat aagtcggaca tcacgcccga ggcaggaacc
      121 atccttgaca gcgccgtgca ggcctatgcc aactgccaga cgatcccgat catgctggct
      181 ggctacaccg accttgccgg cacgcagaag tacaacctgg gtctgtcggc gcgtcgtaac
      241 gcctcggtcc gggcttacct cacctcgcat ggcatcgctg actcggtcat ctcgagccag
      301 gccttcggca agctgcaccc ccgcgtgccg accgccgatg gcgtgcgcga actgcagaac
      361 cgtcgtgtgg aaatcaccta cggtccgggt tcgggcaact gatcggtttc cttgcggaca
      421 ccaagagaga ggccggagca atccggcctc ttttcttttg ggcgaggaag acaggctgag
      481 gcgggcgcga caggggcttg ccgcgccctt ggggcaggca acggacaggt ctgccgggag
      541 gagcttcccc cggcgcaatc gcttatgctg tctgagcgcg cttagagcgc ggcgtagggc
      601 acgccatcca cgcgcccggc cttggcgaag ccttcgaggc gcaggcggca actgtcgcac
      661 ttgccgcagg cggtgccatc gggctgggga tcgtagcacg accaactgat cgccggatca
      721 agccccaggc ggaaggcttc cgagacgatc tcggccttgc cgagatattg cagcggcgcg
      781 tgaatgcgaa aatcatcgcc ttccgcgcca gccttggtgg ccaggcgggc gagatgctcg
      841 aagccgctga tgaattccgg ccggcaatcg ggatagccgg aataatccag cgcattgacg
      901 ccgatgaaca catcgcgcgc gcccagactt tccgcccagg ccagcgtgag cgacagaaac
      961 accagattgc gcgcgggcac ataggtgacc gggatctctt cgccaacgcc ctctttgggc
     1021 acatcgatat ccgccgtgag ggcagagccg ccgaactggc gcagatcgag cggcaggacg
     1081 acatgccggg caacgcccag ttcccgcgcc acggcttgcg cggcatccag ttcgcggcgg
     1141 tggcgctggt tgtagtctat cgtcagcgcg cagatccgat agccggcttc gcgggcgagc
     1201 cctgccgaaa ccatcgaatc gagcccgccc gaaagcagga caaccgccag ccggccctcg
     1261 cccgatgcca aagttgccat gtgagaatat tcctgtgttc agccaagggg gaagggcggc
     1321 tgcagatcag accttaatcg gaaagatccg ggagcagaag gcgcaatcca ccacgatttc
     1381 gcctgcctcg ttgcgcattt cggcccgttc cgcctctgga aactggctca gaatggcctc
     1441 gtaatgcgcc agggtgcagc ggcaaccgcg gtgcaatgcc tgctgcggca gcacacgcac
     1501 ttcacgctct tcatggaaga ggcgccacac cagccaatcc agcggcaagc cctgatcgac
     1561 cagttcggcg ccgcggatgc tgcccgccat gatcgccaca tgctgccatt cgggatgatc
     1621 gaggcggaca tggaggcgct cgcgcccctc ttcgccatcg ggcaggtgct gcatcagcag
     1681 gccgccggcg acatagcctt cctcgccctg ctcaacctcc acacggatca gcgttggcac
     1741 ctgttcggac tggacgaaat agctttcgca ggcggctgcc aacgaggccc cttccagcgg
     1801 cacgatgccc tgataacgct ggcccgacac ggccagatcg aaagtgatcg ccagatagcc
     1861 gctgccgaac agatcggcga tggcgggcgc cgggcccagc gccgccaggg cttcctcgtc
     1921 atgctggaca tagccacgca attcgccttg gcgatagtcg cagaccagga ggctgatcgg
     1981 cccttcggtg gattgggcct gcatggtcag ctggccgtca tcgtccttca gcaggctgcc
     2041 catcagcgcc gtaaccgtca gcgcttcggc cagcaggcgg gccagtaccg gcggataggc
     2101 atgggccgag aggatctcgc gcagcaccgg gcccagccgc accacccgcc cgcgcgcatt
     2161 gcgatcggcc agggtgaaag ccagcaggcc ctcggggctg tgcggctcgg cgggctggtc
     2221 ggcgctcata gctggccgaa ggcccagcgc agcaccgcct tctgggcatg gatgcggttc
     2281 tccgcctcgt cccacacggc cgaatggggc ccgtcgatca ccgcgtcggt cacttcctcg
     2341 ccgcgatggg cgggcaggca gtgcaggaag acggcatcgg cggcggccgc gcgcatcaat
     2401 tcctcgttca cctggaaggg ctgcatggcc gccacatggg cctcgccgcc agcctggccc
     2461 atcgacaccc aggtgtccgt caccaccacc tgggcgccgg ccacggctgc tttgggatcc
     2521 tgcgtgaagg tgatgcggct gcccgcggcc tgcgcggctg ccacgaaagc gccatccggc
     2581 tcataaccgg ccggcccgcc cacgcggatg tggaaaccca gcagccctgc cgcctcgatc
     2641 agcgaatgca gcacattgtt gccatcgccc agccaggcca tttccatgcc ttccagcgcc
     2701 acgccgcgct cggccagcgt cagcagatcg gccatgatct ggcaggggtg cgacaggtcg
     2761 gtcagcccgt tgatcaccgg cacgctggca tagcgcgcca gttcctcgat cttggcatga
     2821 tcgttggtgc ggatcatgat ggcatccacc atgcggctca gcacgcgggc ggtgtcggcg
     2881 atggtctcgc cgcggcccag ctgcatgctg cccgattcca tcacgatcgc gctgccaccc
     2941 agctggcgca tcgccatgtc gaacgagaca cgggtgcggg tggagttctt ctcgaagatc
     3001 atcgccagca catggccggc cagcggcgca tcggcatcgg cccggccctt gggccagctg
     3061 gcgcgggcgg ccttgcgggc gccggcatcg gccagcatgg cccgcagcgc ggccggcccg
     3121 gcatcggcca ggttgaggaa atgccggctc atcacgctgc ctcggccata tagctggcag
     3181 cgcccgccga cagccgctcc atgaactcgt cgatatgcga ctcgtcgatc accagcggcg
     3241 gcagaatgcg catcgtgctg tcgccggccg aaacggtgag cagctggtgc ttgtcgcgca
     3301 gatgcgccac gaagggccgc ggctccttct tcagcttgag gcccagcatc aggccgcgcc
     3361 cgcgcacttc ctcgaacaga tccggataat tgccgatgaa ctgctccaac cggctggtca
     3421 gcaggctgcc cattgcgcgc acatgagcca ggaaatcctc gttggccacc gcatcgagca
     3481 ccgcctcgcc ggctgccatg gccagcggat tgccgccata ggtgctgcca tgcgtgccgg
     3541 ccaccatgcc gcgcgcagcc ttttccctgg caaggcaggc gcccagcggg aagccgccgc
     3601 caatgccctt ggccgtggcc aggatatcgg gcaccacgcc aaactgctca taggcataga
     3661 acgtgccgct gcgggccacg ccgcactgca cttcatcaaa caccagcatc aggtcgtgct
     3721 cgtcgcacag ggcgcgcagg ccctgcagga attcggcgct gggcaccttg atgccgccct
     3781 cgccctggat cggctcgatc atgatgccgc cagtgttggg gccgatcgcc gccttcaccg
     3841 cttccagatc gccgaaatcg acatatttga agccaggcag catgggcaga aagcccttat
     3901 gcatcttttc ctgattgctg gcgctgatcg tgcccatggt gcgcccgtgg aacgcctcct
     3961 tgaaggtgat cagctcgtat ttgtcgagat tgccgacatg ctggtgatag gcgcgcgccg
     4021 tcttgatcgc gcattccacc gcttccgcgc cggaattggt gaagaacacg gtgtcggcaa
     4081 aggtcaggtc caccagccgc tgggccagat gctcaccctg cgggctgcca tagaggttcg
     4141 acacatgcat cagcgtggcc gcctgacgct ggatcgcgcc gatcagcccc tgatgcgagt
     4201 ggcccaggat gttcaccgcg atgccgctgg caaaatccag atatcgttgc ccgtcctcgc
     4261 cgatcagatg gcagtgttca ccgcgcacgg ggcggaagcc gcaccggggg tagacaggca
     4321 tcagcggcgt gatcgacatg gagcattcct cttgcattac aaatagatgc gactggcaac
     4381 acaaatgaca aatggcggtg cgccgggagc gaatccccag cacaccgcca tttgcacgcg
     4441 tttaagtggc ctatggttct tggtcaaacc ccttgacggg agagcgacga agagaggcgg
     4501 tgcggacggg ttcagccctg acgcggcacc aggttgaccg ccgaatactt gcctcgtcga
     4561 tcgacctcga tatcaaattc cagacgatcg ccctcgttca gctcgcgcag gccggaacgc
     4621 tccaccgcgc tgatgtgaac gaacgcatcg ggctgcccat catcacgggt gatgaagccg
     4681 aagcccttca tcgtgttgaa gaacttcacc gtgccggtgg ccttttcgcc ggtcagctgg
     4741 cgctgcggcg caggctcgcg cttggcgacg gggatgacat cgcccatcac gaccagatcg
     4801 ctggccgaga tcttgccacc gcgatcgacg agggtgaact ccaggccctg gccttcggca
     4861 aggccttcca gcccggcacg ctccaccgcg ctgatgtgca cgaacacatc ttcgccgcca
     4921 tcttcgcgct gaatgaagcc gaagcccttc tggccgttga agaacttgac ggtgcccttg
     4981 ctctggccaa cgacctgggc gggcatgccg ccaccgccgc caccgccgcc gcgcgggccg
     5041 ccgaagccgc caccaccgcc gccgccgaag ccgccaccgc gcgggccgcc gccgaagccg
     5101 ccgccgccgc cacgcgggcc accgccgaaa ccgccgcgat caccgccacg atccccgccg
     5161 aagcgatcat ggccgccgaa gcgatcacca ccgcccatga agggatcgaa actgtcttcg
     5221 ccgaacccgt cgcgcttgtc tcggcccctg ccgcgacgtc ctctatcgaa acccataaac
     5281 ccgaaagtac cttcgcttcg cccgaaacca acccactcga tcacgcggac gaatcgcgcc
     5341 gaatgacaag gagaatggcc gcgccagcgc gccatcccac cttttccaaa ttgccacata
     5401 tacgaaaacc gcagccttag cgaataattt tcgcccccgc cccgcgcatt tcgatctttc
     5461 ttgtgacatt tctgccagct gcgataagcc agcggcgcac caacaccggc agggctcccc
     5521 tggcggcggc ctgcctgcct ggccaggccc ccggaaggcg cgcagtttca gccagaaccc
     5581 ggccgccaga atggcgcatt tccgggcaac aggaaaggga atcacatgtt tcgcaagttg
     5641 tccgaagccg tttgggtcag cccgcagatc acgaccgagg acgtggccgc cgctgccgcg
     5701 cagggcatcg ggctgatcat caacaatcgc cccgaaggcg aaagcgacga ccagaccccc
     5761 ggcgatgcca tcgccgacag cgccgcagct ctgggccttg cctatgtcgc cattccggtc
     5821 acccacgccg gcttcagcga aggccaggtg cagcagatgg cccaggccct ggccgaggcc
     5881 aagggcggca ttctggccta ttgccgctcg ggcacccgct ccaccctgct gtgggccctg
     5941 gcgcaggccg ccaccggcac ccatcccgac gatatcgccc gcgccgccgc ccttgccggc
     6001 tatgatatcg gcccggtgcg cgcattggtg gatatgctgg ccgccggcca gaactgatcc
     6061 ctgccccccc gaaggcaaaa gggcgcgagg cggccaggcc cctcgcgccc tttttgcatg
     6121 cctgccctct gcgaacagaa aaatgggccg gaagccaagg cttccgaccc ttaaagttgc
     6181 gttcgcagga ggaacatcgg atggcgggac cgagggggag gagaggagga ggagtccccg
     6241 gccccgccaa actggcaaac tcagtagttg taggcgcgtt cgccgtgttc ggtgatgtcg
     6301 aggccttcgc gctcggcttc ttccgagggg cgcaggccga agatcttgtc gatgatcaca
     6361 tagaggatca gcgaaccgat acccgaccac agcagcgtga tgcccacgcc ctgcgcctgg
     6421 atccacagct ggcccagcat gtcgtagctg cccgtggcgg cggggaactt cgagtagtcg
     6481 gtgatgccga caccgcccag cgacggggca tcgaggatgc cggtcatcag cgcgccgaag
     6541 ataccgccca cgcagtggac accgaagaca tcgagcgtgt cgtcatactt gaagatgttc
     6601 ttcacccagg tgacgaaggc gaagcacagc ggcgagacca gcaggcccag caccaccgaa
     6661 cccatcggcc cggcataacc cgaggcaggc gtcacggcga caagaccagc caccgcaccc
     6721 gaggcagcac ccagcatcga aggcttgccg tggaagatct ggtcgcacag cgcccagctg
     6781 acggcagccg cggcagtggc c
//
LOCUS       DASRYE010000200         9455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_98815,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000200 DASRYE010000000
VERSION     DASRYE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98815"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1800
                     /locus_tag="VFF98_10385"
     CDS             1..1800
                     /locus_tag="VFF98_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZU64081.1"
                     /translation="MQPHEARLAHLRAELARQGLDGFVIPISDEHMSEYVGAYAQRLE
                     WLTGFGGSAGTAVVLKDRAAIFVDGRYTLQVRDQVDGRLYDHLGVPTTSPVRWLAEQA
                     PEGARIGYDPWLHTGGWADGLAKALAARGAEAVAVAANPVDAVWADRPAPGTAPALPH
                     LLAQAGEASADKRVRLATALQADGLDAAVIAALDSLAWLLNLRGSDVACTPVALGFGL
                     LHADGTVDLFIAPEKVGPDLVAHLGDGVRIAPREGFVPALQALGGQRVGVDGERCVAA
                     ISAALAAAGARIVAAQDPCVLPKAIKNAAEQAGHRAAQQRDGAAVARFLHWLSVAAPA
                     GTISEIAAAEALHQFRRDTGLLRDLSFETISGAGPNGAIVHYRVSEETNRLLAPGSVY
                     LVDSGGQYADGTTDITRTVWIGPGAPDAELKDRFTRVLKGHIALARAVFPAGTAGSQL
                     DTLARQHLWAAGLDYAHGTGHGVGSFLAVHEGPQRIAKAAGGQAGTGQALLAGMILSN
                     EPGYYKAGAYGIRIENLVLVEPRAIPGAEGACLGFETLTHVPIERALIAPALLLPEER
                     AWLDAYHAATLALLAPLLEGAALAWLEAQCAPL"
     gene            1984..2742
                     /locus_tag="VFF98_10390"
     CDS             1984..2742
                     /locus_tag="VFF98_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64082.1"
                     /translation="MKSWTIGLAALATAIAGGAIAAEAPQKAPHELTRAEAKDQAEKL
                     FARLDANHDGKLDAADRTARRDALFDRLDTNHDGSLSRAEFDAPPPPPPAQGPDGAKG
                     APGAAPDGPPPAGPDGGHGPHRFGHGGPGHDGQGHDRPDHRGPGHWGPHPGEHMAELG
                     LILQQADPAHTGTITHDAFVNAALALFDKVDTNHDGKISKAEREAARAQWRAQHPGWK
                     HGEHGEHGEHGEHDGPPPMGPDGDMPPPPPPPAK"
     gene            2796..3536
                     /locus_tag="VFF98_10395"
     CDS             2796..3536
                     /locus_tag="VFF98_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU64083.1"
                     /translation="MIQPTGSPVRLLLVDDEAGLREPLAAYLARQGFIVAEAASAAEA
                     RETLREQPVDLVLLDIMMPGEDGLSLCRHLSETRAIPTIFLTARGEPTDRIVGLEIGA
                     DDYVVKPFEPRELVARIRSVLRRSARALAEPAADEDFLFDGWRLDPLKRRLTDPDGAV
                     VAISSVEFRLLMAFVEHPRQVLDRDRLLDMVQGREAHLFDRAVDNQVSRLRRKIEVDS
                     RNPQMIQTVWGGGYMLAADVRRVPSVGE"
     gene            3533..5098
                     /locus_tag="VFF98_10400"
     CDS             3533..5098
                     /locus_tag="VFF98_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64084.1"
                     /translation="MTVAEQPPRRHRLLRLWPASLQGQVLLALALALLLAQGIGAVLR
                     YQLSQQHRREELVNAAAFRLMGALREASQPDHGHDHDHAHDPGHDQGPPDAGHDGPPP
                     PDSGPPPRTDEPRPLHAEWQPAFAPAPADHRWDEEEERLSHILADQGVAVADVRLARR
                     SVADDPVSQVWLMRRWGRLGGHQAPAALGLSAPPKAPTRLLVAAIQPQKNGPWLVARV
                     LIPPAYDAGELLYALIAQTLIIYLVLVGVMALVLRRITRPLAALEKRLIAFAEHRQAD
                     GQLPPEGPEDLRRLILAHNRMEDRIAALLDEKDVMLGAIGHDLKTPLAALRVRIESVE
                     DDTERARMAQTIDEIARTLDDILSLARVGRPSERLEMTELSALVAQVVEDYEDMGEPV
                     LLEEMTRLSLPVRAIWLRRALRNLIGNALRYGGGARVNLARETVAGHSTARITIEDDG
                     PGIPEAAIAAMMEPFRRGEPSRNSLTGGAGLGLTLARAIAEQHGGSLTLANRLDGAGQ
                     IAGLVATLSLPLP"
     gene            5172..7016
                     /gene="pabB"
                     /locus_tag="VFF98_10405"
     CDS             5172..7016
                     /gene="pabB"
                     /locus_tag="VFF98_10405"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZU64085.1"
                     /translation="MAHSPSSAALAPFVLLDDAGGGEDSRARLYRAPGQVIIARRPQD
                     VAPALTAARAALAQGKALAGWLAYEAGLPLEPRLAALCAPRTGADGPLVWLGVFDGWE
                     EMPAAAVPAWLAAETRSPGPHRLGPMEPQLSPGGYARAFAAIRAGIEAGDIYQANFTL
                     PLAGSWRGDPLALYASLRGAGGAAHGGVVFDGANWLLSLSPELFFACADGDIRVKPMK
                     GTRPRGATPDEDAALAAQLRASVKDRAENLMILDLMRNDLARVAEAGSVRVESAFAVE
                     HYPSVHQMVSTATARLAPGLGPVDVLEALHPCGSVTGAPKIRAMELIAQHERDPRGAY
                     CGALGHIDPAGTAAFNVAIRTLRLTGDGQGAASGGGGRAVLGVGSAIVADSDAMDEWR
                     ECMVKGGFVRQAAAGADGAQFDLIETMAFAPDEGIPLLERHLERIAASARALGFAFDR
                     HGARNAIHALCFAAEAPARLRLLLARSGAISLEIGPAPVPFAAPLPCILLPAPVDRLD
                     WRLAHKTSERGFYEAGLAAARAAGAGEALFVGEGGCLTEGCFTSLFVERDGQLLTPPL
                     ADGLLPGVLRAQLLADGRAREARLRPEDLAGGFYLGNALRGLIPAVLL"
     gene            7013..8200
                     /locus_tag="VFF98_10410"
     CDS             7013..8200
                     /locus_tag="VFF98_10410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZU64086.1"
                     /translation="MTDPILSAALARIAPSPTTAMTDRAIALREAGRDIISLSVGEPD
                     FATPDHVVAAAKAALDAGQTKYTAVGGSTQLKQAAALHFERDLGIAAPTGQITVSAGG
                     KQAIFHALLATLNPGDEVLIPAPWWVSYPEIVHFAGATVVPLATTPATGFRLSAEQLA
                     AAITPRTRWLLLNSPGNPTGACYSAEELAALGEVLARHPQVLVMSDDIYAPLRYTPGA
                     HATLSVVRPDLAGRVLTVSGVSKSHAMTGFRIGVATGPAWLIRAMERLQSHSSGNPCS
                     ISQAAAVAAFEGPQAFLADWRERFRTRRDAVVAAINAIPGLATPVPDGAFYCMVDAGP
                     HMARFGDDTALCLHLLEQGVALVAASAFGGGHCGFAGFRISFAADDARLAEALSRIAK
                     ALA"
     gene            8197..8865
                     /locus_tag="VFF98_10415"
     CDS             8197..8865
                     /locus_tag="VFF98_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HZU64087.1"
                     /translation="MTQLPILFEDGEALVIDKPAGLPIDPPRAGGLSLENYLEELKLG
                     FQRPPMPVHRLDRDTSGCLLLARNPKALKRFAAAFEARAVEKRYLGIVAGPVAEDSGT
                     ITLALSKISSAAAGWRMIPAKKGKPAITHWRKLAEVGGHTLVEFLLETGRTHQIRVHA
                     ASGLGTALLGDPIYGDSAGAPRTMLHAASITLTRAGKPPITASAPLPDDFAALGFVDP
                     GHGR"
     gene            8855..9274
                     /gene="arfB"
                     /locus_tag="VFF98_10420"
     CDS             8855..9274
                     /gene="arfB"
                     /locus_tag="VFF98_10420"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZU64088.1"
                     /translation="MADEAEAAVNRAMAALSESFIAAAGPGGQNVNKVATAAQIRVSL
                     YALRLSPPVFQRLKELAGSKVTSAGELVLTARSFRTQEQNRADGRERLSQLIRAAHDL
                     PEKRAKSRLNRVGKAQRLESKKKRGAVKAGRGRVSFD"
BASE COUNT         1332 a         3417 c         3335 g         1371 t
ORIGIN      
        1 atgcagcccc acgaagcccg ccttgcccac ctgcgcgccg aactggcccg gcaggggctc
       61 gatggtttcg tcatccccat ttccgacgag catatgagcg aatatgtcgg cgcctatgcg
      121 caaaggctgg aatggctgac cggctttggc ggatcggccg gcacagcggt ggtgctgaag
      181 gacagggcgg cgatcttcgt cgacgggcgc tacacgctgc aggtgcgcga tcaggtggat
      241 gggcgcctgt atgaccatct tggcgtgccg accacaagcc cggtgcgctg gctggccgaa
      301 caggcgccgg aaggcgcgcg catcggctat gatccttggc tgcacaccgg cggctgggcc
      361 gatgggctgg ccaaggcgct ggccgcgcgc ggggccgagg cggtggcggt ggcggccaat
      421 ccggtggatg cggtgtgggc cgatcggccc gcgcccggca cggcgccggc gctgccccat
      481 ctgctggccc aggcaggcga ggccagcgcc gacaaacgcg tgcgcctggc caccgccctg
      541 caggcagacg ggctggatgc cgcggtgatc gccgcgctcg attcgctggc ctggctgctc
      601 aatttgcgcg gcagcgatgt ggcctgcacg ccggtggcgc tgggctttgg cctgctgcat
      661 gccgatggca cggtggatct gttcatcgcg ccggaaaagg tgggcccgga tctggtggcg
      721 catctgggcg atggggtgcg gatcgccccg cgcgagggct tcgtgcccgc cctgcaggcg
      781 ctgggcggcc agcgcgtggg cgtggatggg gaacgctgcg tggcggccat ctcggccgcg
      841 ctggcggcag cgggggccag gatcgtcgcc gcgcaggatc cttgcgtgct gcccaaggcg
      901 atcaagaacg cggccgaaca ggccggccac cgcgccgcac aacagcgcga cggggccgcc
      961 gtggcgcgct tcctccactg gctcagcgtg gcagcgcccg caggcaccat cagcgagatc
     1021 gccgcggccg aggcgctcca ccagttccgg cgcgacaccg gcctgctgcg cgacctctcc
     1081 ttcgagacga tcagtggcgc cgggcccaat ggcgccatcg tccactaccg ggtgagcgag
     1141 gagaccaacc ggctgctggc gccgggcagt gtctatctgg tcgattcagg cggccaatat
     1201 gccgatggca ccaccgatat cacccgcacc gtgtggattg gccccggcgc ccccgatgcc
     1261 gagctgaagg accgcttcac ccgcgtgctg aaaggccata tcgccctggc ccgcgcggtg
     1321 ttccccgccg gcaccgcggg cagccagctc gacacgctgg cgcgccagca tctgtgggcg
     1381 gccggcctcg attatgccca tggcaccggg cacggggtgg gcagcttcct ggccgtgcat
     1441 gaagggcccc agcgcattgc caaggcggcc ggcgggcagg cgggcaccgg ccaggcgctg
     1501 ctggcgggca tgatcctgtc caacgagccg ggctattaca aggccggcgc ctatggcatc
     1561 cgcatcgaga atctggtgct ggtggagccg cgcgcgattc ccggcgccga gggcgcctgc
     1621 ctcggcttcg agacgctgac ccatgtgccg atcgagcggg cgctgatcgc gcctgcgctg
     1681 ctgctgcctg aggaacgggc ctggctcgat gcctatcatg ccgccacgct ggccctgctg
     1741 gcgccgctgc tggaaggggc agcgctggcc tggctggaag cgcaatgcgc ccccctttaa
     1801 cccgcgcccc ggcgacagat tgcgacaata aaccccaccc ccggcataat cgggcgacat
     1861 atcgcggcta gatccggcac tggaaatcgc gcggccctca tggcaagtcc ccccgcgccg
     1921 ggggccccgc ggtttcttcc ccgccgcccg cgcaagcctg cggcacacag aaggattgcg
     1981 atgatgaaaa gctggaccat cggccttgct gccctggcaa cggcgattgc cggcggcgcc
     2041 attgccgccg aagcgcccca gaaggccccg cacgagctca cccgcgccga ggcgaaggac
     2101 caggccgaga agctgttcgc ccgcctcgat gccaaccacg acggcaagct ggatgccgcc
     2161 gatcgcaccg cccgccgcga cgccctgttc gaccggctgg acaccaacca cgacggcagc
     2221 ctgagccgcg ccgaattcga cgcgccgccg ccgccgccgc ccgcgcaagg ccccgatggc
     2281 gccaagggcg cgcccggcgc tgcccccgat ggccccccgc cggcgggccc ggatggcggc
     2341 cacgggccgc accgcttcgg ccatggcggg cccggtcatg atgggcaggg tcatgatagg
     2401 ccagaccatc gcgggccggg ccattggggc ccccaccccg gcgagcatat ggccgagctg
     2461 ggcctcatcc tgcagcaggc cgatcccgcc cacaccggca cgatcacgca tgatgccttc
     2521 gtgaacgcgg ccctggccct gttcgacaag gtggacacca accacgacgg caagatcagc
     2581 aaggccgagc gcgaagccgc ccgcgcccag tggcgcgccc agcaccccgg ctggaagcat
     2641 ggcgaacacg gcgagcatgg tgaacatggc gagcatgatg gcccgccgcc gatgggcccg
     2701 gatggcgata tgcctccgcc cccgccgccg cctgccaaat aattttttga agaaagccga
     2761 ttgccgatga cccaccccgc cacgaagcca accgcatgat ccagcccacg ggaagcccgg
     2821 tgcggttgct gctggttgac gacgaggcgg gcctgcgcga acccctggcg gcctatctgg
     2881 cccgccaggg gttcatcgtc gccgaggccg ccagcgctgc cgaggcgcgc gaaaccctgc
     2941 gcgagcagcc ggtggatctg gtgctgctcg atatcatgat gcccggcgag gatgggctgt
     3001 cgctgtgccg gcacctgtcg gaaacgcggg cgatccccac catcttcctc accgcccgcg
     3061 gcgagccgac cgaccgcatc gtcggcctgg agatcggcgc cgacgattat gtggtgaagc
     3121 ccttcgagcc gcgcgaactg gtggcgcgca tccgctcggt gctgcgccgt tcggcccgcg
     3181 cgctggcaga gccggcggcg gacgaggatt tcctcttcga tggctggcgg ctcgatccgc
     3241 tgaagcgccg gctgaccgat cccgatggcg cggtggtggc gatctcctcg gtggaattcc
     3301 gcctgctgat ggcctttgtc gaacacccgc ggcaggtgct ggaccgcgac cgcctgctcg
     3361 acatggtgca gggccgcgag gcgcatctgt tcgaccgcgc ggtggacaat caggtgagcc
     3421 gcctgcgccg caagatcgag gtggacagcc gcaacccgca gatgatccag accgtgtggg
     3481 gcggcggcta catgctggcc gccgatgtgc gccgcgtgcc ctcggtgggc gaatgacggt
     3541 ggccgaacag ccgccccggc gccaccggct gctgcgcctg tggccggcca gcctgcaggg
     3601 gcaggtgttg ctggcgctgg cgctggcgct gctgctggcg cagggcatcg gcgcggtcct
     3661 gcgctatcag ctcagccagc agcaccgccg cgaggagctg gtgaacgcgg ccgccttccg
     3721 cctgatgggg gccctgcgcg aggccagcca gcccgaccat ggccatgatc acgatcacgc
     3781 tcacgatccg ggccacgatc agggcccgcc cgatgccggc cacgacggcc cgcccccgcc
     3841 cgacagcggc cccccgcccc gtaccgacga gccccgcccg ctgcatgccg aatggcagcc
     3901 cgccttcgcc cctgcccctg ccgaccatcg ctgggacgag gaggaggagc ggctctccca
     3961 cattctggcc gatcagggcg tggcggtggc cgatgtgcgg ctggcccggc gcagcgtggc
     4021 cgatgatcct gtctcgcagg tgtggctgat gcggcgctgg gggcggctgg gcggccatca
     4081 ggcgccggcc gcgctgggcc tcagcgcgcc gcccaaggcg cccacccgcc tgctggtggc
     4141 cgccatccag ccgcagaaga acggcccctg gctggtcgcc cgcgtgctga tcccgccggc
     4201 ctatgatgcg ggcgaactgc tctatgcgct gatcgcccag acgctgatca tctatctcgt
     4261 gctggtgggc gtgatggcgc tggtgctgcg gcggatcacc cggccgctgg cggcgctgga
     4321 gaagcggctg atcgcctttg ccgaacaccg ccaggccgat ggccagctgc cccccgaagg
     4381 cccggaagac ctgcgccggc tgatcctggc ccacaaccgc atggaagacc gcatcgccgc
     4441 cctgctcgac gagaaggacg tgatgctggg cgccatcggc cacgatctca agaccccgct
     4501 ggcagcgctg cgcgtgcgca tcgaatcggt ggaggacgac accgagcgcg cccgcatggc
     4561 ccagaccatc gacgagatcg cccgcacgct cgacgatatc ctctcgctgg cccgcgtggg
     4621 ccggccgagc gagcggctgg aaatgaccga actgtcggcg ctggtggcgc aggtggtgga
     4681 agattacgag gacatgggcg agccggtgct gctggaggag atgacccgcc tcagcctgcc
     4741 ggtgcgggcg atctggctgc ggcgggcgct gcgcaacctc atcggcaatg cgctgcgcta
     4801 tggcggcggc gcgcgggtga accttgcgcg cgagacggtg gccggccaca gcaccgcgcg
     4861 catcaccatc gaggacgacg gccccggcat ccccgaggcc gcgatcgccg cgatgatgga
     4921 gcccttccgc cgcggcgagc cctcgcgcaa cagcctcacc gggggcgcgg ggctgggcct
     4981 cacgctggcc cgcgccatcg ccgagcagca tggcggcagc ctgacgctgg ccaaccggct
     5041 ggatggcgcc ggccagatcg ccggccttgt cgccaccctc agcctgccgc tgccctgacc
     5101 tgcctttcgg aaaccacgga aacaccgaca gcttttgcat ggcggcggca agccggctag
     5161 aaggctcggc catggcccat tcgccctctt ctgccgccct tgcgcccttt gtgctgctgg
     5221 atgatgccgg cggcggcgaa gattcccgcg cccgcctcta tcgcgcgccc gggcaggtga
     5281 tcatcgcccg ccggccgcag gatgtggcgc cggccctcac cgccgcccgc gccgcgctgg
     5341 cgcagggcaa agcgctggcc ggctggctgg cctatgaggc gggcctgccg ctggagccgc
     5401 gcctggcggc cctgtgcgcg ccgcgcacgg gcgccgatgg gccgctggtg tggctgggcg
     5461 tgttcgatgg ctgggaagag atgccggccg ccgccgtgcc cgcctggctg gccgccgaaa
     5521 cccgcagccc cggcccccac cgcctggggc cgatggagcc gcagctgtcg cccggcggct
     5581 atgcccgcgc ctttgccgcc atccgcgcgg ggatcgaggc gggcgatatc tatcaggcca
     5641 atttcaccct gccgctggcc ggcagctggc gcggcgatcc gctggcgctg tatgccagcc
     5701 tcaggggagc gggcggcgcg gcgcatggcg gggtggtgtt cgatggcgcc aactggctgc
     5761 tcagcctctc gcccgagctg ttcttcgcct gcgccgatgg cgatatccgc gtgaagccga
     5821 tgaagggcac ccgcccgcgc ggcgccaccc cggatgaaga cgccgccctt gccgcccaat
     5881 tgcgcgccag cgtgaaggac cgggcggaaa acctgatgat cctcgacctg atgcgcaacg
     5941 atctggcccg cgtggccgag gccggcagcg tgcgggtgga aagcgccttt gcggtggagc
     6001 attacccctc tgtccaccag atggtgtcca ccgccaccgc gcggctggcg ccggggctgg
     6061 gaccggtgga cgtgctggag gcgctgcacc cttgcggctc tgtaaccggc gcgcccaaga
     6121 tccgcgcgat ggaactgatc gcccagcacg agcgcgaccc gcgcggcgcc tattgcgggg
     6181 cgctcggcca tatcgatccg gctggcacgg ccgccttcaa tgtggcaatc cgcaccctgc
     6241 ggctgaccgg cgacgggcag ggcgcagcca gtggcggcgg gggccgcgcg gtgctggggg
     6301 tgggctctgc catcgtggcg gattccgatg ccatggacga atggcgcgaa tgcatggtca
     6361 aggggggctt cgtgaggcaa gcggcagccg gcgcggatgg cgcccagttc gacctgatcg
     6421 agacgatggc ctttgcgccg gacgagggca ttcccctgct ggagcgccat ctggaacgca
     6481 tcgccgccag cgcccgcgcg ctgggcttcg ctttcgaccg gcatggcgcg cgcaatgcca
     6541 tccatgccct gtgctttgcc gccgaagcgc ccgcccggct gcgcctgctg ctggcccgat
     6601 cgggtgcgat cagcctggag atcggcccgg cgccggtgcc ttttgccgcg cccctgccct
     6661 gcatcctgct gcccgccccg gtggaccggc tggactggcg cctggcgcac aaaacctccg
     6721 agcgcggctt ctacgaggcc ggccttgccg ccgcccgcgc cgccggggct ggcgaggcgc
     6781 tgttcgtggg cgagggcggc tgcctcaccg agggctgctt caccagcctg tttgtggaac
     6841 gcgatggcca gctgctcacc ccgccgctgg ccgatgggct gctgcccggc gtgctgcgcg
     6901 cgcaattgct ggccgatggc cgcgcccgcg aggcgcggct gcggcccgaa gatctggccg
     6961 ggggatttta ccttggtaat gcgctgcgcg ggctgatacc ggccgtgctg ttatgaccga
     7021 tccgatcctg tctgcggcgc tggcccgcat tgccccctcg cccaccaccg cgatgaccga
     7081 ccgcgccatt gccctgcgcg aggccgggcg tgacatcatc tcgctttcgg tgggcgagcc
     7141 cgattttgcc acgcccgacc atgtggtggc agcggccaag gccgcgcttg atgccgggca
     7201 gacgaaatac accgccgttg gcggctcgac ccagctgaaa caggccgccg ccctccattt
     7261 cgagcgcgat ctgggcattg ccgcgcccac cggccagatc accgtttcgg ccggcggcaa
     7321 acaggcgatc ttccacgccc tgctggccac gctgaacccc ggcgacgagg tgctgatccc
     7381 cgcgccctgg tgggtcagct atcccgagat cgtccatttt gccggagcca cggtggtgcc
     7441 gctggcgaca accccggcca ccggctttcg cctttcggcc gagcagctgg cagcggcgat
     7501 caccccgcgc acccgctggc tgctgctgaa cagccccggc aaccccaccg gcgcctgcta
     7561 ttcggccgag gaactggcgg cgctgggcga ggtgctggcc cgccacccgc aggtgctggt
     7621 gatgagcgac gacatctacg ccccgctgcg ctacacgccc ggcgcccatg ccacgctgag
     7681 cgtggtgcgg cccgatctgg ccgggcgcgt gctcaccgtt tccggcgttt ccaaaagcca
     7741 cgcgatgacc ggctttcgga tcggcgtggc caccggccct gcctggctga tccgcgcgat
     7801 ggagcggctg caatcccatt ccagcggcaa cccctgctcg atcagccagg ccgccgctgt
     7861 ggccgccttc gagggcccgc aggcgtttct ggccgattgg cgcgagcgtt tccgcacccg
     7921 gcgcgatgcc gtggtggcgg ccatcaacgc catcccgggg ctggcaacgc cggtgcccga
     7981 tggtgccttc tattgcatgg tcgatgccgg cccccacatg gcccgctttg gcgatgacac
     8041 cgccttgtgc ctgcacctgc tggagcaggg cgtggcgctg gtggcggcca gcgcctttgg
     8101 cggcggccat tgcggctttg ccggcttccg catcagcttt gccgccgacg atgcacggct
     8161 ggccgaggcc ctttcccgca tcgcgaaagc cctggcgtga cgcaacttcc catccttttc
     8221 gaagacggcg aggcgctggt gatcgacaag cccgccggcc tgccgatcga cccgccgcgc
     8281 gctggcggcc tcagccttga gaactatctg gaggaactaa agctgggctt ccagcgcccg
     8341 cccatgcccg tccaccggct ggaccgcgac acttccggct gcctgctgct ggcccgcaac
     8401 cccaaggcgc tgaagcgctt tgccgccgcc tttgaggccc gcgcggtgga aaagcgctat
     8461 ctcggcatcg tcgccgggcc ggtggcggag gacagcggca cgattaccct ggcgctcagc
     8521 aagatcagca gcgcggcggc cggctggcgg atgatccccg ccaagaaggg caagcccgcc
     8581 atcacccatt ggcgcaagct ggccgaagtg ggcggccaca cgctggtgga atttctgctg
     8641 gaaacgggcc gcacgcacca gatccgcgtg catgccgcca gcggccttgg caccgccctg
     8701 ctgggcgatc cgatctatgg cgacagcgcc ggcgcgccgc gcaccatgct gcatgccgcc
     8761 agcatcaccc tcacccgcgc gggcaagccg cccatcaccg ccagcgcgcc gctgccggat
     8821 gattttgccg cgctgggctt tgtggatccg ggccatggcc gatgaggccg aggccgccgt
     8881 caaccgcgcc atggcggcgc tgagcgaaag cttcatcgcc gcggcgggcc ccggcgggca
     8941 gaatgtcaac aaggtggcca ccgccgcgca gatccgcgtc agcctttatg cgctgcgcct
     9001 gtcgccgccg gtgttccagc ggctgaagga actggccggc agcaaggtca cctcggccgg
     9061 cgagctggtg ttgaccgcgc gcagcttccg cacgcaggaa cagaaccgcg ccgatggccg
     9121 cgagcgcctg tcccagctga tccgcgccgc gcatgatctg cccgaaaagc gcgccaagag
     9181 ccggctgaac cgcgtgggca aggcccagcg gctggaatcg aagaagaagc gcggcgcggt
     9241 gaaggccggc cgcggccgcg tcagcttcga ttagagcggc ttgctttaga gcgttttact
     9301 ggggctggtt gccggcgggc tggccggcat cgggggcggg ccgcccttcg gcgctggcgg
     9361 caacggcagc ggcagcggcg gccatgtctt ccggcgcctg gcgggtggag gcggcttcgg
     9421 cggcattggc gccgctatcg ggcgcgggca ccgcg
//
LOCUS       DASRYE010000201        14541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_153156,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000201 DASRYE010000000
VERSION     DASRYE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153156"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFF98_10425"
     CDS             complement(<1..281)
                     /locus_tag="VFF98_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HZU64089.1"
                     /translation="MASNIARITEIVEPEVKALGFDLVRVALFGKTEVGDEEHTLQIM
                     AERPETGQLVMDDCVTLSHRISDRIDALEEAGEVLIHEAYRLEVSSPGI"
     gene            complement(450..1169)
                     /locus_tag="VFF98_10430"
     CDS             complement(450..1169)
                     /locus_tag="VFF98_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU64090.1"
                     /translation="MGKVIAVYSMKGGVGKSSLAVNLAEAAAQKGRALLWDVDAQGAA
                     SFLLGDSARGHKAQRIFAKDIDPAEVVEPTDVPGLDLIAADLSLRHLDAALADDKPKR
                     LRKLLEGLAPDYERIILDCPPGLGELSDQIFRAADILVVPVAPAPLAIRTLDQVRAHL
                     AEARGKKAPVLLPVFSMVDRRKGLHREMLAAHADWPAIPQASVVEQMGVKQAPLVHFA
                     PKSPAAEAVADVWRRVEAALG"
     gene            1304..1735
                     /locus_tag="VFF98_10435"
     CDS             1304..1735
                     /locus_tag="VFF98_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HZU64091.1"
                     /translation="MSTPAPRSLDDIAEEYAFLEPDERYRLLIDLGRALPPMPDALKT
                     DATLVRGCSAAVWVYPAPGEGGRLNFLADSNAAITKGIIALVLSAVQGKPAAEVADMD
                     IAAALAPFDLRNQLSSNRTQGVPNMIALIHGHAARIAGAAE"
     gene            complement(1777..2340)
                     /locus_tag="VFF98_10440"
     CDS             complement(1777..2340)
                     /locus_tag="VFF98_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZU64092.1"
                     /translation="MQAFLAFLIVCASFLVMEGVAWSAHKYIMHGPGWAWHRDHHEPH
                     AGFWEKNDRYALVGAAISITAFALGSGPVMALLGLAAWWPGTWIGLGVLAYGLVYTAI
                     HDGLIHQRWFRYVPHGGYARRLVQAHRLHHAAIGKEGGVSFGFVIAGDPARLKRALAR
                     QRAAGRVVLREGEALREGEALREGEGV"
     gene            2481..3914
                     /gene="leuC"
                     /locus_tag="VFF98_10445"
     CDS             2481..3914
                     /gene="leuC"
                     /locus_tag="VFF98_10445"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZU64093.1"
                     /translation="MSNTPRTLYEKIWDAHVVERRADGTCLIYIDRHLVHEVTSPQAF
                     EALRAAGRKVRRPDLTLAVPDHNLPTTARRTAEGARVPIADAESAQQLEALEANAPAF
                     GIRYIGDADAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEH
                     VLATQTLLLKQSKTMEVRVEGDLPAGISAKDLILHIIGVVGTAGGTGHVIEYRGKVFE
                     DMSIEGRLTVCNMSIEAGARAGLVAPDATTFAYLKGRPYAPKGAEWDKAVAWWQSLAT
                     EEGARFDKSVVINAADIQPTVTWGTSPEDIAPIGGVVPAPESFADASKQDAARKSLAY
                     MGLTPGQKLDEVEIQNVFIGSCTNSRIEDLRAAAAVLKGRHKAANVKWAIVVPGSGLV
                     KAQAEAEGLDKVFIEAGLEWREPGCSACLGMNPDKVPAGERCASTSNRNFVGRQGPGA
                     RTHLVSPAMAAAAAVTGHLTDVRTLLG"
     gene            3934..4110
                     /locus_tag="VFF98_10450"
     CDS             3934..4110
                     /locus_tag="VFF98_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64094.1"
                     /translation="MSDKTEKSSERMGWGGAAALAGAAIGSAALAAALLFANRRKAKP
                     TDTPAHPEHAPETD"
     gene            4125..4718
                     /gene="leuD"
                     /locus_tag="VFF98_10455"
     CDS             4125..4718
                     /gene="leuD"
                     /locus_tag="VFF98_10455"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZU64095.1"
                     /translation="MEAIAEVSGKAIPFGRKNVDTDVIIPARWLKTITREGLGKGAFE
                     SVRAEEGNVFDNPAWAGAPILIAGDNFGCGSSREHAAWALLDMGVNCVIAPSFSDIFS
                     GNAFKNGILTVVLPQAEVDRLLEVAQDHPISVDLESQTVTTPFQDRFSFEIDPFRKHC
                     LLEGLDEVGLTLAQADSISAYEAKAAGNLSFLARAAA"
     gene            4874..5197
                     /locus_tag="VFF98_10460"
     CDS             4874..5197
                     /locus_tag="VFF98_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HZU64096.1"
                     /translation="MTNFQDRERAEEAKFAHDEEMEFRIQARRNRLLGLWAAERMGLS
                     QVEADAYGKSVVQADFEESGDEDVIRKVLGDLVAAGIEAEEAEVRSALKAKEAEARRS
                     VIGGV"
     gene            5253..5486
                     /locus_tag="VFF98_10465"
     CDS             5253..5486
                     /locus_tag="VFF98_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HZU64097.1"
                     /translation="MPMPAPQIEALIRAALPDAEVTITDLAGDGDHYAAHVVSAAFAG
                     KSRIAQHKLVYAAIGPAMGGELHALQVTTAIPN"
     gene            5522..5866
                     /gene="grxD"
                     /locus_tag="VFF98_10470"
     CDS             5522..5866
                     /gene="grxD"
                     /locus_tag="VFF98_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZU64098.1"
                     /translation="MTDTPADPVAQRIADLVEGHDVVLFMKGTPLFPQCGFSSRAVAI
                     LSHLDVPFHAVDVLQDAEIRQGIKAFSDWPTIPQLYVKGEFLGGSDIMMEMFEAGELH
                     ALMAEQGIAAKG"
     gene            6286..7074
                     /locus_tag="VFF98_10475"
     CDS             6286..7074
                     /locus_tag="VFF98_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZU64099.1"
                     /translation="MPASPAPASPIPAATLVIYRRAASGPAEVLMVQRSAQLRFAGGA
                     VAFPGGRVDAADEALAAELAPPGPAETAGERLAETAARIAAIRETLEETGLLLGLAGP
                     VNAADAAAARALLKREGAFAAVLARHGWRLDLAALVPLARWRGPRPPAYDTRFYLADV
                     GTGAQALSADASETDSLFWASPARTLAMAAEGAVSLVFPTRRNLERLAQFAGFTEARA
                     HAEATPRPLILPREEIRAGEPWLVIPEGLGYPVLGEPLAQARRG"
     gene            7251..8840
                     /locus_tag="VFF98_10480"
     CDS             7251..8840
                     /locus_tag="VFF98_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZU64100.1"
                     /translation="MVAAANPLAVAAGLRVLKAGGSAVDAAVAVQAVLGLVEPQSSGL
                     GGGAFLTYYDAKSHRVTAYDGREVAPAGASPRQFLGADGRPLPPFAAMQGGIASGVPG
                     AIAMLEMAQKDHGRLPWRGLFGDARALALKGFVVSPRLASAIRSGAPGAATADAHRYF
                     SRADGTPLQAGDRLANPAYAASLDAIAQKGAAGLLQGPLAAGIIASLQAAPLPSTMTL
                     ADLAAYRPRRAEALCRPYRVYAICTPGAPSGGPAVQEALGLLAHTDIAARRPDDEKAW
                     FLFAQASRLAYADRDRYEGDPTFVRFPQAGLLAPAYLADRAKLIGEVAGPVSWGQPQG
                     APTPGADHTQEPGGTTHFVIVDARGNAVSMTTTVENVFGNGRMVGGFFLNNQLTDFSF
                     SPSDADGTPAANAVAPGKRPRSSMAPTIVLGAGGRLVAATGSPGGSSIIAYTLKSLVA
                     LLDWHMRPQEAAALPNLVARGGSFTADPFPQPIMAGLAARALPLQTARGEASGIHSIL
                     VVNGRYVGGADPRREGQARGF"
     gene            complement(9003..11504)
                     /locus_tag="VFF98_10485"
     CDS             complement(9003..11504)
                     /locus_tag="VFF98_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64101.1"
                     /translation="MIFDDRKAARRLSHRAALALFASPLALAPVFAATAHAGESAGES
                     TGDAAVPASAGDTAGEIVVTATKRSESLQTVPISITAMTAQQLEQHQVAGFDDYAKLL
                     PSVSYQSFGPGQSQLYFRGITNGGDGIASGPLPAAGVYVDETPVTTIFGSVDMHIYDV
                     ARVEALAGPQGTLYGASSLSGTLRIITNQPKLGKWEGGLDGEFNKFGPGSAGGKAEGF
                     INIPIGDRAALRISGYYEHDGGYISNVPGTRTYQRQYTSGYDSGDNPIYATDPMNVSN
                     SKYVAKNFNQVDQAGGRAALRVELDDNWTVTPAVYYQHQTAGGTFLYGPEGYSPGNGT
                     PALGDLQVHDFTPDHDRDDWYLASLTIQGKLSDWDVTYSGSYFDRRVDTVADYSYYTV
                     AYDQLYPDYTHFTDANGKAIDPTQVVDTYNKYTKFSQELRISSPAHNPWRLTAGLFMQ
                     RQTDRFIANYEIPGLSGSQEGGAVYGDDIFYSNLNRVDRDYAAFAEGSYDITRNVTLT
                     AGIRGFIADNTLFGWSGGAGTLSKVAATNNCTGNVTVFSCPDVNSKYVGSGETHKASL
                     KWQVNHNAMVYFTYSTGFRPGGNNRVTYFYGYTQNPGPYKADTLTNYELGWKTTLFDK
                     RLRFNGAIFWEDWNNVQYAQPGIQGVIFTLNAGSARSRGIEGDVSWRVARGLTVSASG
                     TYVDAKLTQDFICPLADGCGNVAYGGLFAAKGTKLPITPSVKLNATARYEFAVGRAKA
                     FLQGSLNSQSSTANSLRNDWQAILGSNKGFTTVDFSGGWTKDNLSFTLFINNAFDERG
                     VLSKNLACSASPCLSYARSYPTKPQQFGIRSSIRF"
     gene            complement(11670..13373)
                     /locus_tag="VFF98_10490"
     CDS             complement(11670..13373)
                     /locus_tag="VFF98_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU64102.1"
                     /translation="MSADLAALRAAALAQPHAPEGWRHLADACFAAGLAAEADAAYQR
                     ATLVAVNDPALRAAGVHLAANRLAEAERLLKPYLKQHPTDVAAIRMLAELAGRIGRLE
                     DAGHLLTRALELAPGFLPARQNMAMLLLRRHRFAEALAEVALLLAAEPENPAFLNLQA
                     VALARIGDYADAVPRFEAVLALRDNQPRLWLSYGHALKTVGRKADAIAAYRRAIALEG
                     ALGEAWWSLANLKALRFSAGDSAAMEKALASGRLGEDDRLHLHFALGKAAEDAGDVPA
                     AFAHYEAANAIRAGQLRYDPAGIEALVDEACATLDPAFFAARAQGGDPAADPIFVLGM
                     PRSGSTLVEQILASHSAVEGTKELPDIEMLARQTGPVDGGHIAAIAALSAPQRAELGA
                     AYLARAGVHRKSGRPLFIDKMPNNWSFVPFIRLILPHAKIIDTRRGAMACCFSNFKQH
                     YARGQAFSYRQDHLARYYRAYVRFMDHMDGVLPGAVHRVSHEAMVADSAREIRALIAH
                     VGLPFEEGCLRFWETERAVQTPSSEQVRRPIFADGVDQWRAFSPFLGPLRQGLGDLAE
                     D"
     gene            complement(13370..13918)
                     /locus_tag="VFF98_10495"
     CDS             complement(13370..13918)
                     /locus_tag="VFF98_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU64103.1"
                     /translation="MSLAEPLLPCVLRAARPADAPALAALKLACFRETFLEGFAIPYP
                     PADRALFEAASYGEGPVAAEIADPAHRTWVMEEEGGALVAYAHVGPAKLPHAEVAPGD
                     GELYQLYLLRRVQGRGLGKVLLDHALDHLAAAGAPVWLGVWSGNLKAQHVYAGRGFAV
                     VGGYQFPVGAWRDDELIMRRPA"
     gene            complement(13967..>14541)
                     /locus_tag="VFF98_10500"
     CDS             complement(13967..>14541)
                     /locus_tag="VFF98_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZU64104.1"
                     /translation="AGAFHDSPAGPRALPGDVLPPFGMVDAAGTLPPPPPLPPGVHLP
                     GPGDPPESTAPGLPLAAATRAAAAAVAACERGGSRVGATVIDSAGEARAMLSADGSNG
                     SHVFVAMRKAEVALAFARPSSQVAAAAARDPGVAARVGPAMFVEGGAVPIFRRGKVIG
                     ALGVSGAGGAVIGRADELCALAGLKALGRR"
BASE COUNT         2420 a         5168 c         4717 g         2236 t
ORIGIN      
        1 tcgatcccgg gcgaggacac ttccaggcga taggcctcgt ggatcagcac ttcgccggct
       61 tcctccagcg cgtcgatccg gtcggaaatg cggtgcgaga gcgtcacgca gtcatccatc
      121 accagctggc cggtctcggg ccgctcagcc atgatctgca gcgtgtgctc ctcatcgccc
      181 acctcggtct tgccgaaaag cgccacgcgc accagatcga aacccagggc cttcacctcg
      241 ggctcgacga tctcggtaat acgcgcgata ttgctggcca taatctcttt cggttcaccc
      301 aaggggcaga cattccggaa atgccaaacc ggccggcccc ttcaaggcgc cagcccggca
      361 aagcattcac aatgtcggga tgaaaagcgc tataggcccc ttgccagcaa attgcaagcc
      421 gcttgggggc cctcttcagc cccccgcctt caccccagcg ccgcctccac gcggcgccac
      481 acatcggcca ccgcttctgc cgccgggctt ttgggggcaa aatgcaccag cggcgcctgt
      541 ttcacgccca tctgctccac cacgctggcc tgggggatgg ccggccagtc ggcatgggcg
      601 gccagcatct cgcggtgcag gcccttgcgc cggtccacca tcgagaacac cggcagcagc
      661 accggcgcct tcttgccgcg cgcctcggcc agatgggcgc gcacctgatc cagcgtgcgg
      721 atcgccagcg gcgccggcgc caccggcacc accagaatat cggccgcgcg gaagatctgg
      781 tccgacaatt cgccaaggcc gggcgggcaa tcgaggatga tccgctcgta atcgggggcc
      841 agcccctcca gcagcttgcg caggcgcttg ggcttgtcgt cggccagcgc cgcatccagg
      901 tggcgcagcg aaagatcggc ggcgataagg tccagccccg gcacgtcggt cggctccacc
      961 acctcggccg gatcgatatc cttggcaaag atgcgctgcg ccttgtgccc gcgcgcgcta
     1021 tcccccagca ggaacgaggc cgccccttgc gcatccacat cccacaacag ggcccgcccc
     1081 ttttgcgccg ccgcttccgc cagattgacc gccagcgagg atttgcccac cccgcccttc
     1141 atgctgtaaa ccgcgatcac cttgcccatc tggtgtcctt ctgccgcccg caatatccaa
     1201 gggttgcgcg cctagcaggc aaaaggcaag cctgtgttac agccggcgcg cctgcgccat
     1261 cgggctggat ttgcgcgcaa agccgggcta ttcgcctcgg cccatgagca cacctgcccc
     1321 ccgcagcctc gacgacatcg ccgaggaata tgccttcctc gagcccgacg agcgctatcg
     1381 cctgctgatc gacctcggcc gtgcgctgcc ccccatgccc gatgcgctga agaccgacgc
     1441 cacgctggtg cgcggctgct cggcggcggt gtgggtctat ccggcgccgg gcgaaggcgg
     1501 ccggctgaac ttcctggccg acagcaacgc ggccatcacc aagggcatca tcgccctggt
     1561 gctgagcgcg gtgcaaggca agccggcggc agaagtggcc gacatggaca tcgccgccgc
     1621 cctcgccccg ttcgatctca ggaaccagct gtcgtccaac cgcacccagg gcgtgcccaa
     1681 catgatcgcg ctgatccatg gccatgccgc gcggatcgcc ggggcggcgg aatagcggaa
     1741 taagcgcaga agcgtgatgc gggggcagcg gggccttcaa accccctcgc cctcccgcag
     1801 cgcctcgccc tcccgcagcg cttcgccttc ccgcagcacc acgcgcccgg cggcccgctg
     1861 gcgcgccagc gcgcgcttca gccgcgccgg atcgccagcg atcacaaagc caaagctgac
     1921 acccccctcc ttgccgatcg ccgcatggtg cagccggtgc gcctgcacca gccgccgggc
     1981 atagccgccg tgcggcacat aacggaacca gcgctggtgg atgaggccat cgtgaatggc
     2041 ggtgtaaacc agcccatagg ccagcacccc cagcccgatc cacgtgcccg gccaccatgc
     2101 cgccagcccc agcagcgcca tcaccgggcc gctgcccagc gcaaaggcgg tgatgctgat
     2161 cgccgcgccc accagcgcat agcggtcgtt cttttcccag aagccggcgt gcggctcgtg
     2221 gtgatcgcgg tgccaggccc agccggggcc atgcatgatg tatttatgcg cgctccacgc
     2281 cacgccctcc atcaccagaa agctggcgca cacgatgaga aaggcgagaa aggcttgcat
     2341 gggcccccta tgcgcccccc gcgccccgcc gccaagagcg gatggggccc ggcacatgcc
     2401 cccgcattcc attgcagcgc cccctgccgg ccctttgtgc tcttgtcatc acgcgcccca
     2461 atgcctaggt cgggcctgtc atgagcaaca ctccccgcac cctttacgag aagatctggg
     2521 atgcccacgt ggtcgagcgc cgcgcggatg gcacctgcct gatctatatc gaccgccatc
     2581 tggttcacga agtcaccagc ccgcaggcgt ttgaagcgct gcgcgcggcg gggcgcaagg
     2641 tgcgccggcc cgaccttacc ctggccgtgc ccgaccataa cctgcccacc accgcccgcc
     2701 gcacggccga aggcgcccgc gtgcccatcg ccgatgccga aagcgcccag cagctggaag
     2761 cgctggaagc caatgcgccg gcctttggca tccgctacat cggcgatgcc gatgcggaac
     2821 agggcatcgt ccatgtcgtc gggccggaac agggcttctc gctgcccggc gccaccatcg
     2881 tctgcggcga cagccacacc gcctgccacg gcggcctggg cgcgctggcc tttggcatcg
     2941 gcacttccga ggtggagcat gtgctggcca cccaaaccct gctgctcaag caatcgaaga
     3001 cgatggaagt gcgcgtggag ggcgatctgc ccgccggcat cagcgccaag gatctcattc
     3061 tccacatcat cggcgtggtg ggcaccgccg gcggcaccgg ccatgtgatc gaatatcgcg
     3121 gcaaggtgtt cgaggacatg tcgatcgagg gccgcctgac ggtgtgcaac atgtcgatcg
     3181 aggcgggcgc gcgcgcaggc ctggtggcgc ccgatgccac cacctttgcc tatcttaaag
     3241 gccgccccta tgcccccaag ggcgccgaat gggacaaggc cgtggcctgg tggcaaagcc
     3301 tggcgaccga ggaaggcgcc cgcttcgaca agagcgtggt gatcaacgcg gccgatatcc
     3361 agcccaccgt cacctggggc accagcccgg aagacatagc gccgatcggc ggcgtggtgc
     3421 ccgcgcccga aagctttgcc gatgcctcca agcaggatgc cgcgcgcaag agcctggcct
     3481 atatgggcct cacccccggc cagaagctgg acgaggtgga gatccagaac gtcttcatcg
     3541 gctcttgcac caacagccgc atcgaggatc tgcgcgcagc cgccgccgtg ctgaagggcc
     3601 gccacaaggc cgccaatgtg aaatgggcga tcgtcgtgcc gggctctggc ctggtgaagg
     3661 cgcaggccga ggccgagggg ctggacaagg tgttcatcga ggccggcctt gaatggcgcg
     3721 agccgggctg ctcggcgtgc ctgggcatga accccgacaa ggtgccggcc ggtgaacgct
     3781 gcgcctccac ctccaaccgc aatttcgtcg gccgccaggg gccgggcgcg cgcacgcatc
     3841 tcgtctcgcc ggcgatggcg gcggccgcgg ccgtgaccgg gcacctcacc gacgtgcgca
     3901 cccttctggg ctgagcaaag caggagcgaa aagatgagcg acaaaaccga aaaatcgtcc
     3961 gagcggatgg gctggggcgg cgcggcggcc ctggctggcg cggcgatcgg atcggcggcg
     4021 ctggccgcgg cgctgctgtt tgccaaccgc cgcaaggcca agcccacaga cacccccgcc
     4081 caccccgaac acgcccccga gacggattga gcggagcgac agacatggaa gcaattgcag
     4141 aggtttccgg caaggcgatc ccgtttggcc gcaagaatgt cgataccgac gtgatcatcc
     4201 cggcccgctg gctgaagacg atcacgcggg aagggctggg caagggcgct ttcgaatcgg
     4261 tgcgcgccga agaaggcaat gtgttcgaca accccgcctg ggcgggcgcg ccgatcctca
     4321 tcgccggcga caatttcggc tgcggctcca gccgcgaaca tgccgcctgg gcactgctcg
     4381 acatgggcgt gaactgcgtc atcgccccca gcttttccga catcttctcg ggcaatgcct
     4441 tcaagaacgg cattctcacc gtggtgctgc cgcaagccga ggtggaccgg ctgctggaag
     4501 tggcgcagga tcatccgatc agcgtggatc ttgagagcca gaccgtgacc acccccttcc
     4561 aggaccgttt ctccttcgag atcgatccgt tccgcaagca ttgcctgctg gaagggctgg
     4621 acgaggtggg gctgacactg gcccaggccg acagcatcag cgcctatgag gccaaggccg
     4681 ccggcaacct gtcgtttctg gccagggccg cggcctgaac gccgatcccg gccgccggcg
     4741 cacagcggat ttgcgccggc gcaaggattg ccgctgcggc cggtgcatga tcggcaccgt
     4801 gcccttccgg gcttgaacca gcgcccgcgc gggattaccc cggccgggcc tgcaattagg
     4861 ggaactgcct gccatgacca attttcagga ccgcgagcgc gccgaagagg ccaagttcgc
     4921 ccatgacgag gaaatggaat tccgcatcca ggcgcggcgc aaccgcctgc tgggcctgtg
     4981 ggcggccgaa cgcatgggcc tctcgcaagt ggaagccgat gcctatggca agagcgttgt
     5041 ccaggccgat ttcgaggaat cgggcgatga ggacgtgatc cgcaaggtgc tgggcgatct
     5101 cgtcgccgcc ggcatcgagg cggaagaggc cgaagtgcgc agcgcgctga aggccaagga
     5161 agccgaggcc cgccgctccg tcatcggcgg cgtctaagat tgcaggatcg cgcgccggcc
     5221 ccagcgccgg cgcccccctc ggagttgccc cgatgcccat gcccgccccc cagatcgaag
     5281 cgctgattcg cgccgccctg cccgatgccg aggtgaccat caccgacctt gccggcgatg
     5341 gcgatcatta tgccgcccat gtcgtttcgg ccgcgtttgc cggcaaaagc cgcatcgccc
     5401 agcacaagct ggtctatgcc gcgatcggcc cggcgatggg cggcgaactg cacgccttgc
     5461 aagtcaccac cgccatccct aactaggcgg ttccttcccc tgttcttgcg tgagcgtaaa
     5521 aatgaccgac acccccgccg atcccgtggc ccagcgcatt gccgatctgg tcgaaggcca
     5581 tgatgtggtg ctgttcatga agggcacgcc gctgttcccg caatgcggct tttccagccg
     5641 ggcggtggcc attctctcgc atctcgatgt gcccttccac gcggtggacg tgctgcagga
     5701 tgccgaaatc cgccagggca tcaaggcctt ctccgactgg ccgacgatcc cccagctcta
     5761 tgtgaagggc gaattcctgg gcggcagcga catcatgatg gaaatgttcg aggcgggcga
     5821 gctgcacgcg ctgatggccg agcagggcat cgccgccaag ggttgaagcc cccttcagac
     5881 tgacaacaag gcgccgcttt ccacccggaa ggcggcgttt tcgtatgggc ctggcaggag
     5941 gagccccaag gaggtggagc cgccgagatc gggaaggcgg ccccacctgt gctcgagatc
     6001 atcagcgacc ggggaagaca gagcttcttg gagaccgggt agtcagctct gcccatcgct
     6061 ttcgagactt cttgggtgca gacaactaag cacaagctgt gccaaacagt caacctgctg
     6121 tttttacgta tatttccaaa atacgcgatg cgcccgccgc ctccccttgt aaaattggcc
     6181 gacaggcctg ccgccgcgcc cctgccgcct tgcgcatttc gcatttgcct catcgccccc
     6241 ctgtggcaca caggcgcccc ccgcccctct ccccagagcc tttgcatgcc cgccagccct
     6301 gcccctgcct ctcccattcc cgccgccacg ctggtcatct atcgccgggc ggccagcggc
     6361 ccggccgaag tgctgatggt gcaaagatcg gcgcaattgc gctttgccgg cggcgcggtg
     6421 gcctttcccg gcgggcgggt ggatgccgcg gacgaagcgc tggcggccga actggcgccg
     6481 cccggccctg ccgaaaccgc cggggaaagg ctggccgaaa ccgccgcccg catcgccgcc
     6541 atccgcgaaa cgctggaaga aaccggcctg ctgctggggc tggcaggccc tgtcaacgcg
     6601 gccgacgccg ccgccgcgcg cgccttgctg aagcgcgagg gggcctttgc cgccgtgctg
     6661 gcccggcatg gctggcggct ggatctggcc gcgctggtgc cgctggcccg ctggcggggc
     6721 ccgcgcccgc cggcctatga cacccgcttc tacctcgccg atgtgggcac cggcgcccag
     6781 gcgctcagcg ccgatgccag cgagaccgac agcctgttct gggccagccc cgcccgcacg
     6841 ctggcgatgg ccgccgaggg cgccgtctcg ctggtgttcc ccacccggcg caatctggaa
     6901 cggctggcgc aatttgccgg ctttaccgag gcccgcgccc atgccgaagc cacgccccgg
     6961 ccgctgatcc tgccgcgcga ggaaatccgc gccggcgagc cctggctcgt tatccccgag
     7021 gggctgggct atccggtgct gggcgaaccc ctggcgcagg cccggcgggg ctgagcccgc
     7081 cccacctgtc aaatttgccg acaccccctt cgcgcccgcc atgcaggagc ctgccgatgc
     7141 ccccgattcc cccccgcaaa gcagccctgg cgctgatgct gtgccttgcc ggcaacgccg
     7201 ctgccaatcc gccccctgcc actccacaag cgggcagcgc cgcccatgcc atggtggccg
     7261 ctgccaatcc gctggcggtg gccgccggcc tcagagtgct gaaggcgggc ggcagcgcag
     7321 tggatgcggc ggtggccgtg caggcggtgc tggggctggt ggagccgcaa agctccggcc
     7381 tgggcggcgg cgccttcctc acctactacg acgccaaaag ccaccgcgtc accgcctatg
     7441 acgggcgcga agtggccccc gccggcgcca gcccgcgcca gttcctgggg gcagacggcc
     7501 gcccgctgcc ccccttcgcg gccatgcagg ggggcattgc cagcggcgtt ccgggcgcca
     7561 tcgccatgct ggaaatggcc cagaaagacc acggccgcct gccctggcgc gggctgttcg
     7621 gcgatgcgcg ggcgctggcg ctgaagggct ttgtcgtctc gccgcggctg gccagtgcca
     7681 ttcgcagcgg cgcgccgggc gctgccaccg ccgatgccca ccgctatttc agccgggccg
     7741 atggcacccc cttgcaggcc ggagaccggc tggccaaccc cgcctatgcc gccagcctcg
     7801 atgccattgc gcaaaagggc gcggccggcc tgctgcaggg gccgctggcc gccggcatca
     7861 tcgccagcct gcaggcagcg ccgctgccca gcacgatgac cctggccgat ctggccgcct
     7921 atcgcccccg ccgcgcagaa gcgctgtgcc ggccctatcg ggtgtacgcc atctgcacgc
     7981 ccggcgcgcc atcgggcggc ccggccgtgc aggaagcgct gggcctgctg gcccacaccg
     8041 acatcgccgc ccggcgcccc gacgacgaga aggcctggtt ccttttcgcc caggccagcc
     8101 gcctcgccta tgccgaccgc gaccgctatg agggcgaccc tacctttgtc cgctttccgc
     8161 aagccggcct gctggcgccc gcctatctgg ccgatcgggc gaagctgatc ggcgaggtcg
     8221 ccggccctgt cagctggggc cagccgcaag gcgcccccac ccccggcgcc gatcacaccc
     8281 aagagcccgg cggcaccacc catttcgtca tcgtcgatgc ccgcggcaat gccgtttcca
     8341 tgacgacgac ggtggaaaat gtgtttggca acgggcgcat ggtgggcggc ttcttcctca
     8401 acaaccagct caccgatttc tccttctcgc ccagcgatgc agatggcacg ccggcggcca
     8461 atgcggtggc gccgggcaag cggccgcgct cgtcgatggc gcccaccatc gtgctggggg
     8521 ctggcgggcg gctggtggcg gccaccggat cgcccggcgg cagctcgatc atcgcctata
     8581 cgctgaagag cctggttgcc ctgctcgatt ggcacatgcg cccgcaagag gccgcggccc
     8641 tccccaatct ggtggcgcgc gggggcagct tcaccgccga tcctttcccg cagccgatca
     8701 tggccggcct tgccgcccgc gccctgcccc tgcaaaccgc gcgcggcgag gcatcgggca
     8761 ttcacagcat tctcgtggtg aacgggcgct atgtcggcgg ggccgatccc cggcgcgagg
     8821 ggcaggcgcg cggtttctaa cgcaaaagcg gcgggaagcg ctctccgctc cccgccgcct
     8881 gggccttgtt tgcaaagcca gcggctggct ttaaaaaagg tggggcccgg gtgctggccc
     8941 gggcccctcg ctccgatcat gcccgggcca gtcccccaac cggccaaggc tcccccgatt
     9001 gttcagaacc ggatactgga gcgaattcca aactgctgcg gcttggtcgg ataggaccgg
     9061 gcataggaca gacacggcga agcgctgcaa gccaggttct tcgacaagac gccgcgctca
     9121 tcgaacgcgt tgttgatgaa cagcgtaaac gatagattat ccttcgtcca gccacctgag
     9181 aaatcaaccg tggtgaaacc cttgttagag ccaaggatcg cctgccagtc gttgcgcaag
     9241 ctgttggccg tgctcgactg gctgttgagc gagccctgca ggaaggcctt ggcccggccc
     9301 acggcaaatt cgtagcgcgc ggtggcgttc agcttcacgc tgggcgtgat cggcagcttg
     9361 gtgcccttgg cggcaaacag cccgccatag gccacattac cgcagccatc ggccagcggg
     9421 cagatgaaat cctgcgtcag cttggcatcc acataggtgc cgctggccga aacggtgagc
     9481 ccgcgcgcca cgcgccagct gacatcgccc tcgatcccgc gcgaacgggc agagccggcg
     9541 ttgagcgtaa agatcactcc ctggatgccg ggctgtgcat attgcacatt gttccagtct
     9601 tcccagaaga tggcgccgtt gaagcgcagc cgcttgtcga acagggtggt tttccagccc
     9661 agctcatagt tggtcagcgt gtcggccttg tagggcccgg ggttctgcgt gtagccgtag
     9721 aaataggtga cacggttgtt gccgcccggg cggaagccgg tggaataggt gaaatagacc
     9781 atcgcattgt ggttcacctg ccacttcagc gaggccttgt gcgtctcgcc gctgccgaca
     9841 tatttggaat tcacatccgg gcaggagaag acggtgacat tgcccgtgca attgttggtg
     9901 gccgccacct tcgacagcgt gcccgccccg cccgaccagc caaacagcgt gttatcggcg
     9961 atgaaaccgc ggataccggc cgtcaacgtt acgttgcggg tgatgtcgta ggagccttcg
    10021 gcaaaggcgg cataatcgcg gtccacgcga ttgaggttgg aatagaagat atcgtcaccg
    10081 taaacggcgc cgccctcctg gctgccgctc aggcccggga tctcgtaatt ggcgatgaaa
    10141 cggtcggtct ggcgctgcat gaacaggccc gccgtcagcc gccaggggtt gtgcgcgggc
    10201 gaggagatgc gcagttcctg gctgaacttg gtgtatttgt tgtaggtgtc caccacctgc
    10261 gtcgggtcga tcgccttgcc gttcgcatcg gtgaagtggg tgtaatcggg atagagctgg
    10321 tcgtaagcca cggtgtaata ggaataatcc gccaccgtat cgacgcgccg gtcgaaatag
    10381 ctgcccgaat aggtcacatc ccagtccgac agcttgccct ggatcgtcag cgaggcgaga
    10441 taccagtcgt cgcggtcgtg gtcgggcgtg aaatcatgca cctgcagatc gcccagcgcc
    10501 ggcgtgccat tgcccggcga atagccttcc gggccataga ggaaagtgcc gccagcggtc
    10561 tggtgctggt agtagacagc gggcgtcacc gtccagttgt cgtccagctc cacgcgcagg
    10621 gccgcgcggc caccggcctg atccacctgg ttgaagttct tggcaacata cttgctgttg
    10681 ctgacattca tcggatcggt ggcatagatc gggttgtcgc cgctgtcata gcccgaggta
    10741 tactggcgct gataggtgcg ggtgcccggc acattgctga tatagccgcc atcatgctcg
    10801 taatagccgc tgatgcgcag ggccgcccga tcgccgatgg ggatgttgat gaagccctcg
    10861 gccttgccgc cggcgctgcc cgggccgaac ttgttgaact cgccgtcaag cccgccttcc
    10921 cacttgccca gcttgggctg gttggtgatg atgcgcagcg tgcccgacag cgagctggcg
    10981 ccatacagcg tgccctgcgg cccggccagc gcctccaccc gggcgacatc gtagatatgc
    11041 atgtcgaccg agccgaagat cgtcgtcacc ggggtttcgt cgacatagac gccggcggcc
    11101 ggcagcgggc ccgaggcgat gccatcgccg ccattggtga tgccgcggaa atagagctgg
    11161 ctctgcccgg gcccgaacga ctggtagctg accgagggca gcagcttggc gtaatcgtcg
    11221 aacccggcga cctggtgctg ttccagctgc tgggcggtca tcgcggtgat gctgatcggc
    11281 accgtctgca gcgattcgga gcgcttggtg gcagtcacca cgatctcgcc ggccgtatcg
    11341 ccagcggagg ccggcacggc ggcatcgcca gtactttctc cggcgctttc tccagcatgg
    11401 gcggtggcgg caaaaaccgg cgccagcgcc agcggcgagg cgaacagggc cagggcggcc
    11461 cgatgcgaaa gccggcgcgc ggctttgcgg tcgtcaaaaa tcatgcatgt cccctgatgg
    11521 cagcttttgc ttttccggcg tccctcaagc cagagcgttc tgccgaaccg acaggcatgt
    11581 caatatgatg gctgtcaacg cacagaaaca ttgtcgccat gttgcaaagc tgccgcattc
    11641 agcgggcagc gccagccctt tgcgccccct caatcctccg ccagatcgcc cagcccttgg
    11701 cgaaggggac caagaaaagg cgaaaacgcc cgccattggt ccaccccgtc ggcgaagatg
    11761 gggcggcgca cctgttcgct gctgggggtt tgcacggcgc gctcggtttc ccagaagcgc
    11821 aggcagccct cctcgaaagg caggccgaca tgggcgatca gcgcgcggat ctcgcgcgcg
    11881 ctgtcggcca ccatcgcctc gtggctgacc cgatgcaccg cgcccggcag tacaccatcc
    11941 atatgatcca tgaaacggac ataggcgcga tagtagcggg ccaggtgatc ctgccggtag
    12001 ctgaacgcct gcccccgcgc ataatgctgt ttgaaattgg aaaagcagca ggccatggcg
    12061 ccgcgccggg tgtcgatgat cttcgcgtgc ggcaggatca gccggatgaa gggcacgaag
    12121 gaccagttgt tcggcatctt gtcgatgaac agcgggcgcc cgctcttgcg atgcaccccc
    12181 gcgcgggcca gataggccgc ccccagctcg gcccgctgcg gggcggaaag ggcggcaatg
    12241 gcagcgatat gcccgccatc caccggccct gtctggcgcg ccagcatctc gatatcgggc
    12301 agttccttgg tgccttccac ggcgctgtgg ctggccagga tctgctccac cagcgtgctg
    12361 cccgaacgcg gcatgcccag cacgaagatg ggatctgccg ccggatcgcc cccttgcgcg
    12421 cgcgcggcga aaaaggcggg atcaagcgtg gcgcaggcct cgtccaccag cgcctcgatg
    12481 ccggccggat catagcgcaa ctgcccggcg cggatggcat tggccgcctc gtaatgggca
    12541 aaggcggcgg gcacatcgcc ggcatcctcg gccgccttgc ccagggcaaa atggaggtgc
    12601 agccggtcgt cctcgcccag ccgtccgctg gccagcgcct tttccatcgc cgcgctgtca
    12661 ccagcggaaa agcgcagcgc cttcagattg gcaaggctcc accaggcctc gcccagcgcg
    12721 ccttccagcg cgatggcacg gcgataggcg gcaatcgcat cggccttgcg ccccaccgtc
    12781 ttcagcgcat ggccatagga cagccacagg cgcggctggt tgtcgcgcag ggccagcacc
    12841 gcttcgaaac gcggcaccgc atcggcataa tcgccgatcc gcgccagcgc caccgcctgc
    12901 aggttgagga aagcggggtt ctccggctct gcggccagca gcagcgccac ctcggccagc
    12961 gcctctgcaa agcggtggcg gcgcagcagc agcatcgcca tgttctgccg ggcgggcaga
    13021 aagccgggcg ccagctccag cgcgcgggtc agcagatggc cggcatcctc cagccggccg
    13081 atgcgccccg ccagttccgc cagcatgcgg atcgccgcca catcggtggg gtgctgcttg
    13141 agatagggct tcagcagccg ctcggcctcg gccagccggt tggcggccag atgcacgccc
    13201 gccgcgcgca gggccggatc gttcaccgcc accagcgtgg cgcgctgata ggcggcatcg
    13261 gcctcggcgg ccagcccggc ggcaaaacag gcatcggcca gatggcgcca gccttccggc
    13321 gcatggggct gggccagggc ggcggcgcgc agggcggcca gatccgcgct catgccggcc
    13381 ggcgcatgat cagttcatca tcgcgccagg cgcccaccgg gaactgatag ccgcccacca
    13441 ccgcaaagcc ccgcccggca tagacatgct gcgccttgag attgccagac cacacgccca
    13501 gccacaccgg cgcgcccgcc gccgccagat gatccagcgc atgatcgagc agcaccttgc
    13561 ccagcccccg cccctgcacc cggcgcagga gatagagctg atacagctcg ccatcgcccg
    13621 gcgccacttc ggcatggggc agcttggccg gccccacatg ggcataggcc accagcgcgc
    13681 cgccttcttc ctccatcacc caggtgcggt gggccggatc ggcgatttcc gccgccaccg
    13741 gcccctcgcc atagctggcc gcctcgaaca gggcgcgatc ggccggcgga tagggaatgg
    13801 caaagccttc gaggaaggtt tcgcggaaac aggccagctt cagcgcggcc agcgccggcg
    13861 catctgccgg gcgggcagcg cgcagaacgc aaggaagaag gggttcggca aggctcatgg
    13921 cggccgcgat acagcaatcg gcaaaagcgc gccagaagga gcgcgcctag cgccggccca
    13981 gcgccttgag gccggccagc gcgcataatt catcggcccg gccgatcact gctccgcccg
    14041 cgcccgaaac gcccagcgcg ccaatcacct tgccccgccg gaagatcggc accgcgccgc
    14101 cttccacaaa catcgccggc cccacgcgcg ccgcaacgcc cggatcgcgc gccgcggctg
    14161 ccgccacttg cgagctgggc cgggcaaagg ccagcgccac ttccgccttg cgcatcgcca
    14221 cgaacacatg actgccattg ctgccatcgg cgctgagcat ggcgcgcgcc tcgccggcgc
    14281 tgtcgatcac cgtagcgccc acgcgtgatc cgccccgctc gcaggccgcc acagcggcgg
    14341 ccgctgcccg cgtggccgcc gccagcggca ggccgggggc ggtgctttcc ggcggatcgc
    14401 ccgggcccgg cagatgcacg cccggcggca gcggcggggg cggcggcagg gtgccggccg
    14461 catccaccat gccgaagggc ggcagcacgt cgcccggcaa ggcgcgcggg cctgccgggc
    14521 tgtcgtggaa ggcgccggcg g
//
LOCUS       DASRYE010000202         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_206144,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000202 DASRYE010000000
VERSION     DASRYE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206144"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFF98_10505"
     CDS             complement(<1..561)
                     /locus_tag="VFF98_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZU64105.1"
                     /translation="MKRAAMKRKAAMPVASAKQRQITTPEGLVLTLTLASRGARLGAL
                     LLDLMLMGGAMVALVIAVIFTAGGMVQLGRHIDGRNAGDHAIQFLLVVLIAGAFLLRN
                     GWFLAFELGPRGATPGKRIMKIRVAARDGRRLSADMVIARNLLRDIEIFLPFSALGAV
                     AAGGQGAAPVAVAAAAWLALWAGFVFF"
     gene            complement(558..1625)
                     /locus_tag="VFF98_10510"
     CDS             complement(558..1625)
                     /locus_tag="VFF98_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZU64106.1"
                     /translation="MSDTLLGALRSRFARRGAAPAPDEAAIAAAALRSDRFRLAREGD
                     WRRLEGIVARMESGRLSGLSDRDVLDLPVLYRQLASSLSIARETSLDLATLSYLEALA
                     QRAWFLIYGPRVSFGGWLRAFFAGGWSRAVRAIAPDICVALAVMVAGTVVGWLLVAHD
                     PQWYGALCAGEQVRVPGASRAVLRGTLFDVRNQNMLGVFAAYLFGHNAQIAILCFALG
                     FALGIPTLLLLVQNTGQLGAMLWLFNGQGLLVDLVGWLCVHGTTELFAILLAGAAGLH
                     VGRSIAFPGALPVMEAAQAAGRRAAQVMLGVVIMLLVAGLLEGFARQLIQDTAGRMAV
                     GGFMLVFWLCWFFVAFRKEQP"
     gene            complement(1622..>2543)
                     /locus_tag="VFF98_10515"
     CDS             complement(1622..>2543)
                     /locus_tag="VFF98_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZU64107.1"
                     /translation="EAAWLRWNGPLGLGARQYRRDVDATIAVVPDIAPLRQGPLQTFL
                     RDSQMGMIARRLRGEGTMFETLAEYQPGMDRRRIDWKVSARHAHLYAKEYEAERNNQI
                     VFAVDCGQAMCEPVAGLSRLDRAVSAALACTWVAVKGGDRVALFGFAAKVRLLTPFAT
                     DTQGFASLRAAAATLDYEAREPNYTLALATLASRLQRRSLIVVFSDFTDPTSAEMMVE
                     AIGRLVARHRVLFVTLRDEELDGIAGAAPADAREVAMAVAADALLRQRALVTGRLRAM
                     GVDVVEAPHTAIGPRLIDAYLALKRAGGIG"
BASE COUNT          453 a          969 c          807 g          314 t
ORIGIN      
        1 gaagaacaca aagccggccc acagcgccag ccaagccgcc gccgcaacgg ccaccggcgc
       61 cgcgccttgc ccgccggccg ccacagcgcc cagcgccgaa aagggcagga atatctcgat
      121 atcgcgcagc aggttgcgcg cgatcaccat gtcggccgaa aggcggcgcc cgtcgcgcgc
      181 ggccacgcgg atcttcatga tgcgcttgcc cggcgttgcc ccgcgcggcc ccagctcgaa
      241 ggccaggaac cagccgttgc gcagcaggaa cgcgcccgcg atcagcacca ccagcaggaa
      301 ctggatggca tggtcgcccg cgttgcggcc atcgatgtgc cgccccagct gcaccatccc
      361 gcccgcggta aagatgaccg caatgaccag cgccaccatc gccccgccca tgagcatcag
      421 gtcgagcagc agcgccccca gccgcgcgcc ccggctggcc agtgtgaggg tgagcaccag
      481 tccctcgggc gtggtgatct ggcgctgctt ggcgctggca acgggcatgg ccgctttgcg
      541 tttcatggca gcgcgcttca tggctgctcc ttgcggaatg ccacgaagaa ccagcacagc
      601 cagaacacca gcatgaagcc gcccaccgcc atccgcccgg cagtgtcctg gatcaactgc
      661 cgcgcaaacc cctcgagcag gcccgccacc aggagcatga tcaccacgcc cagcatgacc
      721 tgcgccgcgc ggcgcccggc ggcctgcgcc gcctccatga cgggcagggc gcccggaaag
      781 gcgatcgagc ggcccacatg cagcccggcc gcccctgcca gcaggatggc gaacagctcg
      841 gtcgtgccat gcacgcacag ccagcccacc aggtcgacca gcaggccctg gccgttaaac
      901 agccacagca tcgcccccag ctgcccggtg ttctgcacca gcagcagcag cgtgggaatg
      961 cccagcgcaa accccagcgc aaagcacaat atggcgatct gcgcgttgtg cccgaacaga
     1021 taggcggcaa acacgcccag catgttctga ttgcgcacgt caaacagcgt tccacgcagc
     1081 accgcgcggc tggccccggg cacgcgcacc tgctcgcccg cgcacagcgc gccgtaccat
     1141 tgcgggtcgt gcgccaccag cagccagccc accaccgttc cggccaccat gaccgccagc
     1201 gccacgcaga tgtcgggggc aatggcgcgc accgcccggc tccacccgcc ggcaaagaag
     1261 gcgcgcagcc acccgccaaa cgacacgcgc gggccataga tcaggaacca ggccctttgc
     1321 gccagcgctt ccagatagct cagcgtggcc agatcgagcg aggtttcgcg cgcgatcgag
     1381 agcgatgagg ccagttgccg ataaagcacc ggcaggtcga gcacatcgcg gtccgacagg
     1441 cccgagaggc gcccgctctc catgcgcgcg acaatgccct cgagccggcg ccagtcgccc
     1501 tcgcgcgcca ggcgaaagcg gtccgatcgc agggcggcgg cggctatggc cgcctcgtcg
     1561 ggggcaggcg ccgcgccccg gcgggcaaag cggctgcgca aggcccccag cagcgtgtcg
     1621 ctcatccgat ccctcctgcc cgtttgagcg ccagataggc gtcgatcaac cttggcccga
     1681 tggccgtgtg cggcgcctcg accacatcga cgcccattgc ccgcaggcgc cccgttacca
     1741 gcgccctttg ccgcagcagc gcgtcggccg ctaccgccat ggccacctcg cgcgcatcgg
     1801 ccggggccgc gccggcaatg ccgtccagct cctcgtcgcg cagggtgacg aacagcacgc
     1861 ggtggcgcgc caccagccgg ccaatcgcct cgaccatcat ttcggcgctg gtggggtcgg
     1921 taaaatcgga aaacacaacg atgagcgagc ggcgctgcaa ccggctggcc aaagtggcca
     1981 gcgccagcgt gtagttgggt tcgcgcgctt cgtaatccag cgtggcggct gccgcgcgca
     2041 agctggcaaa cccctgcgta tcggtggcaa agggggtgag caggcgcacc ttggcggcaa
     2101 agccgaacag cgccacccgg tcgccgccct tgaccgccac ccacgtgcag gccagcgccg
     2161 ccgacactgc ccgatccagc cgcgaaagac ccgccaccgg ctcgcacatg gcctgcccgc
     2221 aatccacggc aaacacgatc tggttgttgc gctcggcctc gtattccttg gcatacaggt
     2281 gggcgtggcg ggccgaaacc ttccagtcga tgcggcgccg gtccatgccg ggctgatatt
     2341 cggccagcgt ctcgaacatg gtgccctcgc cgcgcaggcg gcgggcgatc atgcccatct
     2401 ggctgtcgcg cagaaaggtt tgcagcggcc cctgccgcag cggcgcaatg tcgggcacca
     2461 cggcaatggt ggcatccaca tcgcggcgat attggcgcgc gcccagcccc agcggcccgt
     2521 tccagcgcag ccacgccgcc tcg
//
LOCUS       DASRYE010000203        10127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_223377,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000203 DASRYE010000000
VERSION     DASRYE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223377"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFF98_10520"
     CDS             <1..549
                     /locus_tag="VFF98_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64108.1"
                     /translation="RLPADLDARLTLFAQAVEEALAQEAWDEVLLVAHSQGAVWAVEV
                     LARVLARRGGALPAGFALVTLGGCIPLLAARRDAAGFAAALARVRAADPAWLDLASPA
                     DGACAGLMPPGSAPPPPRFTQRNPRWYRYAATRGGQDRLAVHFNYLRRLDRPSPLDYP
                     ALALAAQPLAASIAAFDAAHAG"
     gene            546..1913
                     /locus_tag="VFF98_10525"
     CDS             546..1913
                     /locus_tag="VFF98_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU64109.1"
                     /translation="MSTCPFTPPYPRPLASKAGLLRRFWRGWHSWVHTLFAQSYRMKM
                     GQVRLPRLTLHVVNAPELVEEIMADPGGAFPKHPLLADMLEPLLGASLFTANGPHWAA
                     QRRMMGPAFAQAHLDRSFAPMAAACAGLMADLAHADLAAPLDIDPLMTHVTADVIMRT
                     LFSTPLAEGPARRLHRTFARYQAWAQRAALLGLYGLPGLGCGGRARRAGAEIRALFAP
                     LVAARLQAPPPYPAQDMLDALIAARHPETGAPFSQGDLVDQAAILFLAGHETSATALT
                     WALYLLAESPELQERLHAEVGAVAGSGPIDAAAVKRLEGVRNLFREALRLYPPVSFLP
                     RRAARGALLRGRAVGPEDMLVISPWLIHRHADHWPCPHAFDPDRFARPEGQAAAAHAF
                     LPFGKGPRICIGAGFAMQEAVLILASVIRAYRLRYPAGPKPQLTSRLTLRPRHGMPLW
                     LAPRG"
     gene            complement(1921..2331)
                     /locus_tag="VFF98_10530"
     CDS             complement(1921..2331)
                     /locus_tag="VFF98_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU64110.1"
                     /translation="MSPDDTPFPAPDPQPADSRIAARESLLLMADCRLVGDARIVRVK
                     LRNLSASGIMAESQLVPARGAAIAIDLRNIGWVEGVVAWVQAPRFGVAFRQEINPALV
                     RQLPASDQPDCIVRRPLAVRLKADRIDPGRIRSI"
     gene            2582..2893
                     /locus_tag="VFF98_10535"
     CDS             2582..2893
                     /locus_tag="VFF98_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZU64111.1"
                     /translation="MQTLRSPHRLLRESLVQGVALCCLLVMLALAVAGPSGLLAWQEN
                     RHDLTLRQQQIAQLTHQRDQLKNRVALLDPRHADPDLAGELLRRNLGVAHPDEMIIIV
                     H"
     gene            3066..4139
                     /gene="pdhA"
                     /locus_tag="VFF98_10540"
     CDS             3066..4139
                     /gene="pdhA"
                     /locus_tag="VFF98_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZU64112.1"
                     /translation="MAKSARTQAAATAAAEDASNPLHALQAAYEANNRYGASDEELLH
                     FYEQMVLIRRFEEKAGQLYGLGLIGGFCHLYIGQEAVAVGIQSALQAGHDSVITGYRD
                     HGHMLAYGIDPKVIMAELTGREAGISRGKGGSMHMFSTEHKFFGGHGIVGAQVSLGAG
                     LAFAHNYRADGGLCVAYFGDGAANQGQVYESFNMAALWKLPIIFVVENNQYAMGTAVK
                     RSSAETHFHRRGAAFTIPGMDVNGMDVLEVRAAANVAVEYVRAGNGPVLMELNTYRYR
                     GHSMSDPAKYRSREEVQDVREHRDPIEHAKKDLMERGIPEEKLKDIEKRIRQICAEAA
                     DFAETSPEPAPGELHTDVLVESY"
     gene            4141..5487
                     /locus_tag="VFF98_10545"
     CDS             4141..5487
                     /locus_tag="VFF98_10545"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HZU64113.1"
                     /translation="MAIELKMPALSPTMEEGKLAKWLVKVGDEVRSGDILAEIETDKA
                     TMEFEAVDEGTVGALLVAEGTEGVKVGTVIALIGGEGEAAVPAAAAPAPAPVAAAAAA
                     VAAPAPVARPADPVVPHGTNMKTSTVREALRDAMAEEMRRDERVFVIGEEVAEYQGAY
                     KVTQGLLEEFGARRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIIN
                     SAAKTNYMSGGQMRCPVVFRGPNGAASRVGAQHSQNYGPWYANVPGLIVIAPYDASDA
                     KGLLKAAIRSEDPVVFLENELVYGRTFELPELDDHVLPIGKARIMREGKDVTIVSYSI
                     GVGMALDAAEQLAAEGIDAEVVDLRTLRPLDKATVLASLKKTNRIVVAEEGWPQCSIA
                     SEIAAIVMEEGFDDLDAPVVRVCNEDVPLPYAHNLEKAALIDASRIVAAVKKVCYR"
     gene            5598..6248
                     /locus_tag="VFF98_10550"
     CDS             5598..6248
                     /locus_tag="VFF98_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64114.1"
                     /translation="MTELTDSLPPLQRLALAYAPAPTRALWAALLALDGRLAGVVRQV
                     GLAGKEPMIAQLRLAWWRDRLQEPAAQWPAGEPLLAALAGWQGQHGALAALVDGWEHL
                     LAEAPLPAYAMTAWADGRAHAASGLAALLGADAQAAAALARAWSLADLAAHLSAPEEQ
                     AEARRLWQEAPLPPRAPRALRPLAVLGGLARREAAGTPARGVNGLILALRLGLWGA"
     gene            6294..6857
                     /locus_tag="VFF98_10555"
     CDS             6294..6857
                     /locus_tag="VFF98_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HZU64115.1"
                     /translation="MNRIILGAVGALLLAAAGLFWWQGRAQLEKGAPPPDISASRAAS
                     GPVELPTAGAHGRGPGLPGAGKATQTTEQKRFNRFDKNHDGRITRNEMLSTRVAAFKK
                     LDVNHDNLLTFEEWAVKTANRFKEVDRNGDGIITRDELDAYYAAQDAKKAERAKERAG
                     RCACAPDGPAGKARATGKPPADDDEDQ"
     gene            complement(6861..8222)
                     /gene="trmFO"
                     /locus_tag="VFF98_10560"
     CDS             complement(6861..8222)
                     /gene="trmFO"
                     /locus_tag="VFF98_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZU64116.1"
                     /translation="MADDIHIIGGGLAGSEAAWQLARRGLKVRLSEMRGGAQGLRSPA
                     HQGDGLAELVCSNSLRSDDHDSNAVGLLHHEMRQCGSLIMAAADRARVPAGSALAVDR
                     DVFSAEIEAALTAQPTLTLARERVDSLPPAGLTIIATGPLTAAALADSIGAATGASSL
                     AFFDAIAPIVYHETIDMDIAWRAARWDKGAEASIALGSDGKDYINCPMDKTQYLAFRE
                     ALLAGEKTVFKEWEANTPYFDGCMPIEVMAERGEDTLRFGPMKPVGLDNPRTGRWPYA
                     VVQLRQDNKLGTLWNMVGFQTKLKYAEQTRVFRLIPGLEKAEFARLGGLHRNTFLNSP
                     VLLDRQLRLKSAPHIRFAGQITGCEGYVESASVGMLAGLMAAAELTGRAWSPPPATTA
                     LGALLAHITGDAEADTYQPMNINFGLFPPVVHERKVKKPLRKLAYTARAKADIAPWLA
                     TLD"
     gene            complement(8225..8581)
                     /locus_tag="VFF98_10565"
     CDS             complement(8225..8581)
                     /locus_tag="VFF98_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HZU64117.1"
                     /translation="MTIAWKILTTDQWTTWQQAGLFAGAPVDLADGYIHLSAEAQLAG
                     TLDKWFAGQEGLVLAAVDLAAAEAALPGAVRWEAARGGALFPHVYGALPLAVVRGHGP
                     VARAADGAVVLPALPA"
     gene            complement(8578..>10127)
                     /locus_tag="VFF98_10570"
     CDS             complement(8578..>10127)
                     /locus_tag="VFF98_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HZU64118.1"
                     /translation="NLDEVVRIIRGAPNPAAARAALLAREWPMGEIASYIRLVEAIEE
                     GADELSAPTYRLSEVQVKAILDLRLHRLTALGRDEIGDELEGLAKAIAEYLSILADRA
                     KLYGVMREELVVVKAAFATPRVSEITGSAEGIEDEDLIERDDMVVTVTMDGYIKRTPL
                     STFRAQNRGGKGRAGMATKEEDAVATMFVTTTHNPVLFFSTAGKVYRLKVYKLPEGGP
                     ATRGRPIVNLLPALDQGETIATVLPLPEDEAEWDKLSVVFATALGNIRRNSMDAFANI
                     PSNGKFAMRFEDGSADRLIGVGLLTEDDDVLLASRQGKAIRFAADEVREFASRTSTGV
                     RGMALKGDDEVISLSILHRVGATSEEREDYLKFAPWKGEKEGEPAVPAERMAELAARE
                     QFLLTVCANGYGKLSSAYEYRRTGRGGQGITNIDNIARNGAVVASFPANQADQLMLVT
                     DQAKLIRLPLESLRVIGRGSAGVRLFNVGAGEHVVSAVRLAEEEGAEEDGGVEGSTGA
                     ETGTTEA"
BASE COUNT         1582 a         3411 c         3504 g         1630 t
ORIGIN      
        1 cgcctgcccg ccgatctcga cgcgcggctg acgctgtttg cccaagctgt cgaggaagcc
       61 ctggcgcaag aagcgtggga cgaggtgctg ctggtggccc attcgcaagg ggcggtttgg
      121 gcggtggagg tgctggcccg ggtgctggcc cgcaggggcg gggcgctgcc ggccggtttc
      181 gcgctggtca cgctgggcgg ctgcattccg ctgctggcgg cgcggcgtga tgcggcaggc
      241 tttgccgcgg cgctggcgcg ggtgcgggcg gcagatcccg cctggctcga tctcgcctcg
      301 ccggcagatg gggcctgcgc ggggctgatg ccgcccggca gcgccccgcc gccgccgcgc
      361 ttcacccagc gcaatccgcg ctggtatcgc tatgccgcca cgcgcggcgg gcaagacagg
      421 ctggcggtcc acttcaacta tctgcgccgg ctcgatcggc cttcgccgct ggattatccg
      481 gcgctggcgc tggccgcgca gccgctggcg gcctccatcg ccgccttcga tgcggcgcat
      541 gccggatgag cacctgcccc ttcacgccgc cctatccccg cccgctggcc agcaaggccg
      601 ggctgctgcg gcgcttctgg cgcgggtggc acagctgggt gcacacgctt ttcgcccaga
      661 gctaccgcat gaagatgggg caggtgcgcc tgccccgcct gaccctgcat gtggtgaacg
      721 cgccggaact ggtggaggag atcatggccg atccgggcgg ggcctttccc aagcacccgc
      781 tgctggccga tatgctggaa cccttgctgg gcgccagcct cttcaccgcc aacggcccgc
      841 attgggcggc gcagcggcgg atgatggggc ccgccttcgc gcaggcgcat ctcgatcgca
      901 gctttgcgcc catggcggcg gcctgcgccg ggctgatggc ggatttggcc cacgcggatc
      961 tggccgcgcc cctcgatatc gacccgctga tgacccatgt gaccgccgat gtgatcatgc
     1021 gcacgctgtt ttccaccccg ctggcagaag ggccggcgcg gcgcctgcac cggaccttcg
     1081 cgcgctatca ggcctgggcg caaagggcgg cgctgctggg cctctatggc ttgccggggc
     1141 tgggctgtgg cggccgggca cggcgggcag gggcagagat ccgcgcgctg ttcgcaccgc
     1201 ttgtcgccgc ccggttgcag gccccgccgc cctatcccgc gcaagatatg ctggacgcac
     1261 tgatcgccgc ccgccacccg gaaacgggcg cgcccttcag ccagggcgat ctggtcgatc
     1321 aggcggcgat cctgttcctg gccgggcatg aaacttcggc cacggcgctc acctgggcgc
     1381 tctatctgct ggcggaaagc ccggagttgc aggagcgcct gcatgccgaa gtgggcgcgg
     1441 tggcgggcag cggcccgatc gatgccgcgg cggtgaagcg gctggaaggc gtgcgcaacc
     1501 tgttccgcga ggcgctgcgg ctgtatccgc cggtcagctt tctgccgcgg cgggcggcgc
     1561 gaggggcgtt gctgcgcggg cgcgcggttg gccctgagga tatgctggtt atctctccct
     1621 ggctgatcca ccgccatgcc gatcattggc catgccccca cgctttcgat cccgatcggt
     1681 ttgcccggcc ggaagggcag gcggcagctg cccatgcctt tctccctttc ggcaaggggc
     1741 cgcgcatctg catcggcgcg ggctttgcca tgcaggaagc ggtgttgatc ctggccagcg
     1801 tgatccgcgc ctatcgcctg cgctatccgg ccggccccaa gccacagttg accagccggc
     1861 tgaccctgcg cccgcgccat ggcatgccgc tgtggctggc gccgcgcggg tgagcgcagg
     1921 ctagatcgag cggatgcggc cggggtctat cctgtcggcc ttgaggcgca cggccagcgg
     1981 gcggcgcacg atacaatcgg gctggtcgct ggcgggcagc tggcgcacca gggcagggtt
     2041 gatctcctgg cggaaggcca cgccaaagcg cggcgcctgc acccaggcca ccaccccttc
     2101 cacccagccg atattgcgca gatcgatggc aatcgcggcg ccccttgccg gcaccagctg
     2161 gctttccgcc atgatgccgc tggccgacag gttgcgcagc ttcacccgca cgatgcgcgc
     2221 atcgcccacc agccggcaat cggccatcag cagcaggctt tcgcgcgcgg caatgcggct
     2281 gtcggcaggc tgcgggtcgg gcgcggggaa gggggtgtcg tctgggctca tcgcattgcc
     2341 tgttgtgggc gcctgtcgtg gaccggaaag gcacgccgcg cgccattttc cgggccgccc
     2401 gaaactaggc gccaaaggca aagacgggct tgggcccggc ataggggttt gcccctgaat
     2461 gcggccccgg gggatgccgg ccggcgtttt tccaccaaaa aacgccgcag ggcctggccg
     2521 agcaccgaaa accgcaattt atggcttgaa actgggattc aactgtgatt cccttccatt
     2581 catgcaaacg ctgcgttctc ctcatcgcct gctgcgtgaa agccttgttc agggggtggc
     2641 gctgtgttgc ctgctggtga tgctggcgct ggcggtggcc gggcccagcg gcctgctggc
     2701 ctggcaggag aaccgccacg atctgaccct gcgccagcaa cagatcgccc agctgaccca
     2761 ccagcgcgac cagttgaaga accgcgtggc cctgcttgat ccgcgccatg ccgatccgga
     2821 tctagcgggt gaattgctgc gccgtaatct gggcgtggcg caccccgacg agatgatcat
     2881 catcgtccat tgaggagggc ggaaccggtt ccttatcgcc tgcctttcac acatatataa
     2941 ccgatatggt tttaacctat cggaactgct tgccagccat tacaaaattc gtatgcacct
     3001 gtttccgggc gcagggcttg cgcctataga ccggcgccac tccagcctta tacggggaag
     3061 tcgacttggc caaatccgcc agaacccagg ccgcagccac tgccgcagcc gaagatgcca
     3121 gcaaccctct ccacgccctt caggccgcct atgaagcgaa caatcgctat ggcgcttcgg
     3181 acgaggaact gcttcatttc tacgaacaga tggtgctgat ccgccgcttc gaggaaaagg
     3241 ccggccagct ctatggcctg ggcctgatcg gcggcttctg ccacctctac atcggccagg
     3301 aagcggttgc ggtgggcatc cagtcggcgc tgcaggccgg ccatgacagc gtgatcaccg
     3361 gctatcgcga ccatggccac atgctggcct atggcatcga ccccaaggtg atcatggccg
     3421 agttgaccgg ccgcgaggct ggcatcagcc gcggcaaggg cggctcgatg cacatgttct
     3481 ccaccgagca caagttcttc ggcggccatg gcatcgtcgg cgcgcaggtg tcgctgggcg
     3541 cgggcctggc ctttgcccat aactatcgcg ccgatggcgg cctgtgcgtg gcctatttcg
     3601 gcgatggcgc ggccaaccag ggccaggtct acgagagctt caacatggcc gctctctgga
     3661 agctgccgat catcttcgtg gtcgagaaca accagtatgc catgggcacg gcggtgaagc
     3721 gttcctcggc ggaaacccat ttccaccgtc gcggcgccgc gttcaccatc ccgggcatgg
     3781 atgtgaacgg catggacgtg ctggaagtgc gcgcggccgc caatgtggcg gtggaatatg
     3841 tgcgcgccgg caatggcccg gtgctgatgg aactgaacac ctatcgctat cgcggccact
     3901 cgatgtccga tccggcgaaa tatcgcagcc gcgaggaagt gcaggatgtg cgcgagcatc
     3961 gcgatcccat cgagcatgcc aagaaggacc tgatggagcg cggcatcccc gaggaaaagc
     4021 tgaaggacat cgagaagcgc atccgccaga tctgcgccga agcggccgat ttcgccgaaa
     4081 cctcgcccga gccggcccct ggcgaactgc ataccgacgt actggtggag agctactgag
     4141 atggcaatcg aactgaagat gccggcgctc tcgccgacga tggaagaggg caagctggcc
     4201 aagtggctgg tgaaggtggg cgatgaggtc cgctcgggcg atatcctcgc cgagatcgag
     4261 accgacaagg cgacgatgga attcgaggcg gtggacgagg gcaccgtggg cgcgctgctg
     4321 gtggccgagg gcaccgaggg cgtgaaagtg ggcacggtga tcgcgctgat cggcggcgag
     4381 ggtgaggcgg ctgtgccggc cgcagccgcc ccggcgccgg cccctgtggc agcggctgct
     4441 gcggccgtgg cggcgcccgc gccggtggcc cgcccggccg atccggtggt gccccatggc
     4501 accaacatga agacctccac cgtgcgcgag gccctgcgcg atgccatggc cgaggaaatg
     4561 cgccgcgacg agcgcgtctt cgtgatcggc gaggaagtgg ccgaatatca gggcgcctac
     4621 aaggtgaccc agggcctgct ggaagagttc ggcgcgcgcc gcgtgatcga tacgccgatc
     4681 accgaatatg gctttgccgg catcggcacc ggcgcggcga tgggcggcct gcgcccgatc
     4741 gtcgagttca tgacgttcaa cttcgccatg caggcgattg accacatcat caactcggcc
     4801 gccaagacga actacatgtc gggtggtcag atgcgctgcc cggtggtgtt ccgcggcccc
     4861 aatggtgcgg ccagccgcgt cggcgcgcag cactcgcaga actatggccc gtggtatgcc
     4921 aatgtgcccg gcctgatcgt catcgcgccc tatgacgcga gcgatgccaa gggcctgctc
     4981 aaggccgcca tccgcagcga ggatccggtc gtgttcctcg agaacgagct ggtctatggc
     5041 cgcaccttcg agctgcccga gctggacgac cacgtgctgc cgatcggcaa ggcccgcatc
     5101 atgcgtgagg gcaaggacgt gaccatcgtc agctactcga tcggcgtggg catggcgctg
     5161 gacgccgccg agcaactggc cgccgaaggc atcgacgccg aggtggtgga cctgcgcacg
     5221 ctgcgcccgc tcgacaaggc gacggtgctg gcctcgctga agaagaccaa ccgcatcgtg
     5281 gtggccgaag aaggctggcc gcagtgctcg atcgcctcgg aaatcgccgc catcgtgatg
     5341 gaagagggct tcgatgatct cgacgcgccg gtggtccgcg tgtgcaacga ggatgtgccg
     5401 ctgccctatg cgcacaacct cgaaaaggcc gcgctgatcg atgcctcgcg catcgtggcg
     5461 gcggtcaaga aggtgtgcta ccgctaagcg gcagcgctgc ctcgcgtttt ggcgcgcagg
     5521 cacatctgcc aaagggaagg ggcatcgcca tgcgcggtgc ccctttgccg tgttattaca
     5581 ggaaccagga accgcgcatg accgagctga ccgacagtct gcctcccttg caaaggctgg
     5641 cgctggccta tgcgccggcg ccgacgcgtg ccctgtgggc ggcattgctg gcgctggacg
     5701 ggcggcttgc cggcgtggtg cggcaagtgg gcctggccgg caaggagccg atgatcgccc
     5761 agctgcgcct ggcgtggtgg cgcgatcggc tgcaggagcc ggcggcgcaa tggccggcgg
     5821 gcgagccgct gctggcagcg ctggccggat ggcaggggca gcatggcgcg ctggccgcgc
     5881 tggttgacgg gtgggagcat ctgctggccg aagcaccgct gcccgcctat gcgatgaccg
     5941 cctgggccga tggccgggcg cacgccgcca gcgggctcgc cgccctgctg ggggccgatg
     6001 cccaggccgc cgcggcgctg gcccgcgcgt ggagcctggc cgatctggcc gcccacctgt
     6061 ccgcgcccga ggagcaggcc gaggcccgcc gcctgtggca ggaagccccg ctgccccccc
     6121 gcgcgccgcg cgccctacgc ccgctggcgg tgctgggcgg cctggcccgg cgcgaggcgg
     6181 ccggtacccc ggcaaggggg gttaacggat tgattctggc tttaaggctt ggcttgtggg
     6241 gcgcctaagg taaaacccat atccggtgac gaggcagggg ggctggaaac gtcgtgaacc
     6301 ggatcattct cggggctgtg ggggcgctgc tgttggcagc ggcggggctg ttctggtggc
     6361 agggccgcgc ccagctggaa aagggcgcac cgccgcccga tatttccgcc agtcgcgccg
     6421 cctcagggcc ggtggagctg cctaccgccg gcgcccatgg ccgcgggccg ggcctgcccg
     6481 gcgctggcaa agccacccag accaccgagc agaagcgctt caaccgcttt gacaagaacc
     6541 acgatggccg catcacccgc aacgagatgc tgagcacgcg cgttgccgct ttcaagaagc
     6601 tggatgtgaa ccacgacaac ctcctcacct tcgaggaatg ggccgtgaag accgccaacc
     6661 gcttcaagga agtggatcgc aacggcgatg gcatcatcac ccgcgacgag ctggacgcct
     6721 attacgccgc gcaggatgcc aagaaggccg agcgcgccaa ggaacgcgcc ggccgctgcg
     6781 cctgcgcgcc cgatggcccc gccggcaagg cgcgcgccac cggcaagccc ccggcagacg
     6841 acgacgagga ccagtaggcg ctagtccagc gtggccagcc agggggcgat atcggccttg
     6901 gcgcgggcgg tgtaggccag cttgcgcagc ggcttcttca ccttgcgctc atgtaccacg
     6961 ggcgggaata ggccgaaatt gatgttcatc ggctgatagg tgtcggcctc ggcatccccg
     7021 gtgatatggg ccagcagcgc gcccagcgcg gtggtcgccg gcggcgggct ccaggcgcgg
     7081 ccggtcagct cggcggcggc catcagcccg gccagcatcc ccacactggc gctttccaca
     7141 tagccctcgc agccggtgat ctggccggca aagcggatat gcggcgccga tttcagccgc
     7201 agctgccggt cgagcagcac gggggaattg aggaaagtgt tgcgatgaag gccgcccagc
     7261 cgggcaaatt ccgccttctc cagcccgggg atcaggcgga acacgcgggt ctgctcggca
     7321 tatttgagct tggtctggaa gcccaccatg ttccacagcg tgcccagctt gttatcctgc
     7381 cgcagctgca ccaccgcata gggccagcgc ccggtgcgcg gattatccag ccccaccggc
     7441 ttcatcgggc caaagcgcag cgtatcctcg ccgcgctcgg ccatcacctc gatcggcatg
     7501 cagccgtcga aatagggggt gttggcctcc cattccttga acacggtctt ctcgccggcc
     7561 agcagtgcct cgcggaaggc gagatactgg gtcttgtcca tcgggcagtt gatgtaatcc
     7621 ttgccgtcgg agcccagcgc gatgctggcc tcggcgccct tgtcccagcg ggcggcgcgc
     7681 caggcgatgt ccatgtcgat cgtctcgtgg tagacgatgg gggcgatggc atcgaagaag
     7741 gccaggctgc tggcaccagt ggcagcgcca atgctgtctg ccagggcggc ggcggtgagc
     7801 ggcccggtgg cgatgatggt gaggcccgct ggcggcaggc tatccacccg ttcgcgcgcc
     7861 agggtgaggg tgggctgggc ggtcaacgcc gcctcgatct cggcggagaa cacgtcgcgg
     7921 tccaccgcca gcgcgctgcc ggcgggcacg cgggccctgt cggccgcggc catgatcagc
     7981 gagccgcact ggcgcatctc gtggtgcagc aggccgaccg cattgctgtc atgatcgtcg
     8041 gaacgcaggg aattggagca gaccagctcg gccaggccat cgccctgatg ggccgggctg
     8101 cgcaagcctt gcgctccgcc gcgcatttcc gacaggcgca ctttgaggcc ccggcgggcc
     8161 agctgccagg cagcctcgct gccggcaagg ccgccgccga tgatgtgaat gtcgtcagcc
     8221 atggtcaggc cggcagggca ggcagcacaa ccgcgccatc agccgcccgc gccacgggcc
     8281 cgtggccgcg caccaccgcc agcggcaagg cgccatagac atgcgggaac agcgcccccc
     8341 cgcgcgccgc ttcccagcgc accgcgccgg gcagggccgc ttctgccgcc gccagatcga
     8401 cagcggccag caccaggcct tcctgcccgg caaaccactt gtccagcgtg ccggccagct
     8461 gcgcttcggc cgacaggtgg atatagccat cggccagatc caccggggcg ccggcaaaca
     8521 ggcccgcctg ctgccaggtg gtccattgat cggtggtcag gatcttccag gcgatggtca
     8581 tgcctcggtg gtcccggtct cggccccggt gctgccttcc acgccgccgt cttcctcggc
     8641 gccttcttcc tcagccaggc gcacggccga gacgacatgc tcgcccgcgc ccacgttgaa
     8701 cagccgcaca ccggccgaac cgcgcccgat cacccgcagg ctttccagcg gcaggcggat
     8761 gagcttggcc tgatcggtca ccagcatcag ctgatcggcc tgattggccg ggaagctggc
     8821 caccaccgcg ccattgcggg cgatattgtc gatattggtg atgccctggc caccgcgccc
     8881 ggtgcgccgg tattcatagg cagacgacag cttgccatag ccattggcgc aaacggtgag
     8941 gaggaactgc tcgcgcgcgg caagttcggc catgcgttcg gccggcacgg cgggctcgcc
     9001 ttccttctcg cccttccagg gggcgaattt cagataatcc tcacgctcct cgctggtggc
     9061 gcccacgcgg tggaggatgg agagcgagat cacctcgtcg tcccccttca gcgccatgcc
     9121 gcgcacgccg gtggacgtgc gcgaggcaaa ttcgcgcact tcatcggcgg caaagcggat
     9181 ggccttgccc tgccggcttg ccagcagcac atcgtcgtcc tcggtcaaca ggcccacgcc
     9241 gatcaggcgg tcggcgctgc cgtcctcgaa gcgcatggcg aatttgccgt tggaggggat
     9301 gttggcgaag gcatccatgc tgttgcggcg gatgttgccc agcgccgtgg caaacaccac
     9361 cgacagcttg tcccattccg cctcgtcttc cggcagcggc agcacagtgg cgatcgtctc
     9421 gccctgatcg agcgccggca gcaggttgac gatgggccgc ccgcgcgtgg ccggcccgcc
     9481 ttccggcagc ttgtagacct tgaggcgata gaccttgccg gcggtggaga agaacagcac
     9541 cggattgtga gtggtggtaa cgaacatcgt cgccaccgca tcctcttcct tcgtcgccat
     9601 gccggcgcgg cccttgccgc cgcggttctg cgcacggaag gtggagaggg gggtgcgctt
     9661 gatgtagccg tccatcgtca ccgtcacgac catgtcgtcg cgctcgatga gatcctcgtc
     9721 ctcgatcccc tcggcagagc cggtgatctc ggacacgcgg ggcgtggcaa aggcggcctt
     9781 caccaccacc agctcctcgc gcatcacgcc ataaagcttg gcgcggtccg ccaggatcga
     9841 gagatattcg gcgatggcct tggccaggcc ttccagctcg tcgccgatct cgtcgcggcc
     9901 aagggccgtc aggcggtgga ggcgcaggtc gaggatcgcc ttcacctgca cttcggacag
     9961 gcgataggtg ggggcggaaa gctcgtccgc gccttcctcg atggcttcca ccaggcggat
    10021 ataggaggcg atctcgccca tcggccattc gcgcgccagc agcgcggcgc gggcggcggc
    10081 cgggttgggc gcgccgcgga tgatgcgcac cacctcatcg aggttgg
//
LOCUS       DASRYE010000204         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_12037,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000204 DASRYE010000000
VERSION     DASRYE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12037"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VFF98_10575"
     CDS             <1..1374
                     /locus_tag="VFF98_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HZU64119.1"
                     /translation="RILAAWLQANTAIPARPGKGAAWSVDNAAHRLLGWLCQAPLVLS
                     GQDKALRGQLLEAIGATARWLDLNVDRGEDVLARVAGWVAIVAAGLLLPDGRARRLYG
                     EAGLVAALGELVGDDGGVLSRSPLAQMEAIGLLVDLAACYRASRRDPPAAIEGIVQML
                     VPPLLALMHGDGGLGSWQGGWAVSAEALAGLIGASGVRTRPLRDVRQWGFQRVTAGKS
                     LLLFDAAPPPLPRHARHGCASTLAFEFGHLGQRIIVNCGGAAAAGGLMPVRLEQGLRA
                     TAAHSTLVLDDANSTAVTIHGGLGSGVTEVEVDRRQMAWDNGPRGGMSATRLEASHDG
                     YAARYGLVHRRILLLRDDGTELRGEDMLLPSGRKGKRGVVSFAIRFHLGPGIEAGRSG
                     DGQGVGLALPDGTYWQFRSGGGEVTIEESIWADEHGRPVPVQQLVVQGKLSRGGGNFA
                     WLLKKMG"
     gene            1531..3105
                     /gene="purH"
                     /locus_tag="VFF98_10580"
     CDS             1531..3105
                     /gene="purH"
                     /locus_tag="VFF98_10580"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZU64120.1"
                     /translation="MTDTVTIRRALLSVSDKTGLVELGQALSRQGVELVSTGGTAKAL
                     RDAGLDVKDISELTGFPEMMDGRVKTLHPKVHGGLLAVRDNAEHVASMDEHAIGAIDL
                     VVVNLYPFVQTVARGGSREDIIENIDIGGPSMVRSAAKNHDFVAIVTDPADYALVAGG
                     ETTLAQRRKFAAKAYALTAAYDGAISQWFATVDQGETFPETLAITGARVSELRYGENP
                     HQQAALYVPHGAREKGLAQAVQVQGKELSYNNYNDADAALELLAEFRDADPTVVIVKH
                     ANPCGVATAATLAEAYEAAFQCDSVSAFGGIIAVNRPLDGPTAEAISGIFTEVVVAPD
                     ANDDAKAVFAKKKNLRLLLIGEIPDAKRGGLVAKPIAGGLLVQNRDNGAISLDDLKVV
                     TRRQPSAQELADALFAWTVAKHVKSNAIVYARDGVTAGIGAGQMNRRDSSRIAAAKAA
                     EAAETHGFTETRTKGSAVASDAFFPFADGLLAAVEAGATMVIQPGGSMRDEEVIKAAD
                     EAGLAMVFTGMRHFRH"
     gene            3252..3398
                     /locus_tag="VFF98_10585"
     CDS             3252..3398
                     /locus_tag="VFF98_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64121.1"
                     /translation="MGLFKSDLYRSFAIGFVLGAIGICVLLGPGAGSGAGNVVPAAVA
                     APSH"
     gene            3486..3986
                     /locus_tag="VFF98_10590"
     CDS             3486..3986
                     /locus_tag="VFF98_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZU64122.1"
                     /translation="MGSLSSPERHSHHEHSGAGLTEAARDVLIAAGEQWTQMRADVFA
                     QLAGHERPASAYDIAEGVSALRGKRVAANSVYRILDLFVRTNLARRVESANAYVANSH
                     PGCRHDCIFLICDRCGKVVHIDDDALTGALIGAARAAGFADVRPVVELRGLCAECGAH
                     AALPQG"
BASE COUNT          651 a         1329 c         1496 g          705 t
ORIGIN      
        1 cgcattctgg ccgcctggct gcaggccaac accgccatcc ccgcccgccc cggcaagggc
       61 gcggcgtgga gcgtggacaa tgccgcccac cgcctgctgg gctggctgtg ccaggcgccg
      121 ctggtgctgt cgggccagga caaggcgctg cgcgggcaat tgctggaagc gattggcgcc
      181 accgcgcgct ggctcgatct taacgttgat cgcggcgagg atgtgctggc gcgggtggcc
      241 ggctgggtgg cgattgtggc cgccggcctg ctgctgcccg atggccgcgc ccgccgcctg
      301 tatggcgagg ccgggctggt ggcggcgctg ggcgagctgg tgggcgatga tggcggcgtg
      361 ctgtcgcgca gcccgctggc gcagatggag gcgattggcc tgctggttga tctggccgcc
      421 tgctatcggg ccagccgccg cgatccgccg gccgcgatcg agggcattgt gcagatgctg
      481 gtgccgccgc tgctggcctt gatgcatggc gatggcgggc tgggcagctg gcagggcggc
      541 tgggcggttt cggccgaggc gctggccggg ttgatcggcg ccagcggcgt gcgcacgcgg
      601 cccttgcgcg atgtgcggca atggggcttc cagcgagtga cggcgggcaa gagcctgctg
      661 ttgttcgatg ccgcgccccc gccgctgccg cgccatgccc gccatggctg cgcgtccacg
      721 ctggcgttcg aatttggcca tctgggccag cggatcatcg tcaattgcgg cggggcggcg
      781 gcggctggcg ggctgatgcc ggtgcggctg gaacagggcc tgcgggcaac cgctgcccat
      841 tccacgctgg tgcttgatga tgccaattcc accgctgtca ccatccatgg cgggctgggc
      901 agcggcgtga ccgaggtgga agtggaccgc cgccagatgg cgtgggacaa tggcccgcgc
      961 ggcggcatgt cggccacccg gctggaggcc agccacgatg gctatgccgc gcgctatggg
     1021 ctggtgcacc ggcgcatcct gctgctgcgc gatgatggca ccgaattgcg cggcgaggac
     1081 atgctgctgc cttcgggccg caagggcaag cgcggggtgg tctcctttgc catccgcttc
     1141 catctgggcc cgggcatcga ggcgggccgc tcgggcgatg gccagggggt ggggctggcg
     1201 ctgcccgatg gcacttactg gcagttccgc tcgggcgggg gcgaggtgac gatcgaggaa
     1261 tcgatctggg ccgacgagca tggccggccg gtgccggtgc agcagctggt ggtgcagggc
     1321 aagctgtctc gcgggggcgg caatttcgcc tggctgctga agaagatggg ctgagcggcc
     1381 ggctgttaag ctgcgcatct tgcagggcaa atgggcgatt gcccccttgg cccttcccaa
     1441 acccgccaaa atccttatga ggcgcggcaa aagggccccg ccgaagtggg cccgccgttc
     1501 gcgcaagatc cagcccagaa aggccccctc atgaccgata ccgtcacgat ccgtcgtgca
     1561 ttgctctccg tttccgacaa gacggggctt gtcgaactgg gccaggcgct gagccggcag
     1621 ggggtggagc tggtctctac cggcggcacc gccaaggcgc tgcgcgatgc cgggctggac
     1681 gtgaaggaca tctccgagct gaccggcttt cccgagatga tggacggccg ggtgaagacg
     1741 ctgcacccca aggtgcatgg cggcctgctg gccgtgcgcg acaatgccga gcatgtggca
     1801 tcgatggatg agcacgccat cggcgcgatc gatctggtgg tggtgaacct ctaccccttc
     1861 gtgcagacgg tggcgcgtgg cggcagccgc gaggacatca tcgagaacat cgacatcggc
     1921 ggccccagca tggtgcgttc ggcggccaag aaccatgatt tcgtcgccat cgtcaccgat
     1981 ccggccgatt atgcgctggt ggccggcggc gagaccacgc tggcccagcg ccgcaagttt
     2041 gccgccaagg cctatgcgct gacggctgcc tatgatggcg cgatcagcca gtggtttgcc
     2101 actgtcgatc agggcgagac cttccccgag acgctggcga tcaccggcgc ccgcgtgtcg
     2161 gaactgcgct atggcgagaa tccgcatcag caggcggcgc tgtatgtgcc ccatggcgcc
     2221 agggaaaagg gcctggccca ggccgtgcag gtgcagggca aggagctgag ctacaacaat
     2281 tacaacgacg ccgatgccgc gctcgaactg ctggccgaat tccgcgatgc cgatcccacg
     2341 gtggtgatcg tcaagcatgc caacccttgc ggcgtggcca ccgcggccac gctggcagaa
     2401 gcctatgagg ccgcgttcca gtgcgattcg gtcagcgcgt tcggcggcat catcgccgtc
     2461 aaccgcccgc tcgatggccc gacggccgag gcgatcagcg gcatcttcac cgaagtcgtc
     2521 gtggcgcccg atgccaatga cgatgccaag gcggtgtttg ccaaaaagaa gaacctgcgc
     2581 ctgctgctga tcggcgagat ccccgatgcc aagcgcggcg gccttgtcgc caagccgatt
     2641 gccggcggcc tgctggtgca gaaccgcgac aatggcgcga tcagccttga tgacctcaag
     2701 gtcgtcaccc ggcgacagcc cagcgcacag gaactggccg acgcgctgtt cgcctggacg
     2761 gtggccaagc atgtgaagtc gaacgccatc gtctatgcca gggacggcgt gacggcgggc
     2821 attggcgcag gccagatgaa ccgccgcgat tccagccgca ttgccgccgc caaggccgca
     2881 gaagccgccg aaacccacgg ctttaccgaa acccgcacca agggcagcgc ggtggcttcc
     2941 gatgcctttt tcccctttgc cgatggcctg ctggccgcgg tggaagcggg cgccacgatg
     3001 gtgatccagc cgggcggcag catgcgcgac gaggaagtga tcaaggcggc cgacgaggcg
     3061 ggcctggcca tggtgttcac cggcatgcgt cacttccgcc actgatgccc gggggccacg
     3121 ccggctgcgg cttgtcgcag ccggcgcgcg gctggtcgac aagcgcctgt gcaactgcgg
     3181 taggggtctg gaaattaacc agcagatacc cacataggcg tccttaacga atctcaagca
     3241 tgctgggcaa aatgggcctg ttcaaatccg acctgtatcg ttccttcgcc atcggttttg
     3301 tgttgggtgc catcggcatc tgcgttctgc ttggccccgg ggccggcagc ggcgcgggca
     3361 atgtcgtgcc cgcggcggtg gccgcgccct cgcactgagt tttccgggcg ggctgccatg
     3421 ccgggaaggg cccgccaggg gcgcagcttt tgccttggcg gaaaatcagg ctatagctgc
     3481 cggccatggg cagtctgtct tctcctgaac gccattccca tcacgagcac agcggcgccg
     3541 gcctgaccga ggccgcgcgc gacgtgctga tcgccgccgg cgagcaatgg acgcagatgc
     3601 gcgccgatgt gttcgcgcag ctggccgggc acgaacggcc cgcttccgcc tacgacatcg
     3661 ccgagggggt ttcggccctg cgcggcaagc gggtggcggc caacagcgtc tatcgcatcc
     3721 tcgatctgtt cgtgcgcacc aatctggcgc gccgggtgga aagtgccaac gcctatgtgg
     3781 cgaacagcca tccgggctgc cggcatgatt gcattttcct catctgtgat cgctgcggca
     3841 aggtcgtcca tatcgacgat gatgccctca ccggcgcgct gatcggcgcg gcgcgggcgg
     3901 ccggctttgc cgatgtgcgc ccggtggtgg aattgcgcgg gctgtgcgcc gagtgtggcg
     3961 cccatgcagc actgccacag ggttgacctt gacgcatgat gcattgcatc atgagagatc
     4021 gacactgcgt gccggaacgg gtaaatacgc ccgatggcca atagcacaaa gcccgcgctt
     4081 ctcgataata tcgccacgcc gtcggacctt cggggtttga gtgtatcgga gctggttgaa
     4141 ctggcgggcg aggtgcgtga tgagatgatc cactcggtgg g
//
LOCUS       DASRYE010000205         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_223466,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000205 DASRYE010000000
VERSION     DASRYE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223466"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1430
                     /locus_tag="VFF98_10595"
     CDS             222..1430
                     /locus_tag="VFF98_10595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZU64123.1"
                     /translation="MVQLLTRPASASRLAGTLTVAPTTKPRARRVAKPRVPKVQHVGP
                     HPDLPLGQILGGDCVAAMRALPSASVDMIFADPPYNLQLGGDLARPDGSHVDAVTNDW
                     DKFDSFAAYDAFTRDWLAEARRVLKPNGSLWVIGSYHNIFRVGATLQDMGFWILNDIV
                     WRKANPMPNFKGTRFTNAHETLIWASMGEKSKYTFNYRAMKTLNDELQMRSDWVLPIC
                     NGAERLKKGGTKVHPTQKPESLLYRLMLATTNKGDVVLDPFFGTGTTGAVAKRLGRHW
                     IGCEREGGYREAALERIELALPLDESSLVTMQSPKAAPKVAFGTLLETGWLKPGLELF
                     DKKNRFRARVRADGSLVSGQVTGSIHSAGAQLQGAPSCNGWTFWHLEHEGQVKPLDAI
                     RQLYILATEP"
     gene            1427..>2003
                     /locus_tag="VFF98_10600"
     CDS             1427..>2003
                     /locus_tag="VFF98_10600"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZU64124.1"
                     /translation="MSEAVYLRPLLLADSPQAVGGAAVRLAGGLAYASHIALIRREGG
                     RVVARSVLEAGAVPAAMAALPPALKAEAEAQWAALQAPRAPLHLGARTIRLDQPQVMG
                     ILNVTPDSFSDGGQWMERPGVAEEHAAAMLEAGAALIDIGGESTRPGAPPVWEGDEMK
                     RVIPLVAHLARAGAAISLDTRRLAVMEEAVAA"
BASE COUNT          332 a          657 c          695 g          319 t
ORIGIN      
        1 gtagcggcgg gcggaaacgg cgggcggcaa aaaaattccc gcccctgcgg caccacatat
       61 cgagtctcgc accgtggcgc agcccccata tagggtggca gactctgtct gttccccttt
      121 ctgactcatt gcgaatcgcg ggatttgcac gagtcgcgcc ttgacgcgga gtcggcgatg
      181 aatcatttta tggggccctt tgcagatatc cacaggttca tatggtccag ttgctcaccc
      241 ggccggcatc cgcttcccgc cttgccggca ctctgacggt tgcccccacc accaagccgc
      301 gcgcccgccg cgtggccaag ccgcgcgtgc ccaaggtgca gcatgtgggg ccgcatccgg
      361 atctgccgct gggccagatc ctgggcggcg attgcgtggc ggcgatgcgc gccctgccgt
      421 ccgccagcgt cgacatgatc ttcgccgatc cgccctacaa cctgcagctg ggcggcgatc
      481 tggcgcggcc cgatggcagc catgtcgatg cggtgaccaa cgactgggac aagttcgaca
      541 gcttcgccgc ctacgacgcc ttcacgcggg actggctggc cgaggcgcgc cgggtgctca
      601 agcccaatgg cagcctgtgg gtgatcggca gctatcacaa catcttccgc gtgggcgcca
      661 cgctgcagga catgggcttc tggatcctca acgacattgt ctggcgcaag gccaacccca
      721 tgcccaattt caagggcacg cgcttcacca acgcgcatga gacgctgatc tgggcgagca
      781 tgggcgagaa gtcgaaatac accttcaact atcgcgcgat gaagacgctg aacgatgagt
      841 tgcagatgcg ctcggactgg gtgctgccga tctgcaatgg cgctgaacgg ctgaagaagg
      901 gcggcaccaa ggtgcacccc acgcaaaagc cggaaagcct gctctatcgg ctgatgctgg
      961 ccactaccaa caagggcgat gtggtgctcg atcccttctt cggcaccggc accacagggg
     1021 ccgtggccaa gcggctgggc cgccactgga tcggctgcga gcgcgagggc ggttatcgcg
     1081 aagccgcgct ggaacgcatc gagctggcgc tgccgctcga tgaatcgagc cttgtcacga
     1141 tgcagagccc caaggccgcg cccaaggtgg cctttggcac gctgctggaa accggctggc
     1201 tgaagccggg ccttgaactg ttcgacaaga agaaccgctt tcgcgccagg gtgcgggccg
     1261 atggctcgct ggtgtcgggg caggtcaccg gctcgatcca ttcggccggc gcgcaattgc
     1321 agggcgcgcc cagctgcaac ggctggacct tctggcatct ggagcatgag gggcaggtga
     1381 agccgctcga tgcgatccgc cagctctaca tcctcgccac cgaaccatga gcgaggcggt
     1441 atacctgcgc ccgctgctgc tggcagacag cccgcaggcg gtgggcgggg cggcggtgcg
     1501 gctggcaggc gggctggcct atgccagcca tatcgcgctt atccgccgcg agggcggccg
     1561 cgtggtggcc cgcagcgtgc tggaggcggg cgccgtgccc gctgccatgg cggccctgcc
     1621 gccggcgctg aaggccgagg cagaggcgca gtgggccgct ttgcaggccc cgcgcgcacc
     1681 gctgcatctg ggcgcgcgca ccatccggct ggatcagccg caggtgatgg gcatcctcaa
     1741 cgtcacgccc gacagttttt ccgatggtgg ccagtggatg gagcgccccg gcgttgccga
     1801 agaacatgcc gcagccatgc tggaggcggg cgccgcgctg atcgacattg gcggcgagag
     1861 cacccgcccc ggcgcgcccc cggtgtggga gggcgacgag atgaagcggg tgatcccgct
     1921 ggtggcgcat ctggcgcggg cgggcgcggc gatcagcctc gacacgcgcc gcctggcggt
     1981 gatggaagag gcggtggctg ccg
//
LOCUS       DASRYE010000206         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_27059,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000206 DASRYE010000000
VERSION     DASRYE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27059"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFF98_10605"
     CDS             <1..692
                     /locus_tag="VFF98_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64125.1"
                     /translation="ELRALIGAFNAMQQRIDGLLDANAQTMLAIGHDLRTPLARLQLR
                     IDTLRLPADDRAALEADVDEMRDLLGSLQAYVEGDGAGQRREPVDLAAMAQTLVDQAT
                     EQGAEASYAGPPRVVVDAHATALRRALANLLGNALQYGGRARVRLRQQGSMIEIAIED
                     DGPGIAEAHLAKVLQPFVRLDHARERNTRGMGLGLAIVDKAVRAQGGTLVLENRPAGG
                     LVARICLPARG"
     gene            864..2126
                     /locus_tag="VFF98_10610"
     CDS             864..2126
                     /locus_tag="VFF98_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU64126.1"
                     /translation="MALAFWPLALAWAGGAAAGAGGAGEAYSLAALEELARTGSPALQ
                     AAAAGVDGARAGVETARAFPNPEVEYLAGNTRYRPGVDGVSGAVGSIGLSQPLDLPFR
                     RTPRIAAARAGLAAAHAGYAAIEADWIADLRRAYFDMLRRAAETANAGEDLALMQSVQ
                     AKIALRVQKGDAPRFEMIRAEADLLNVRKAAQAAALREEQARLRLRALVGPDLPADFA
                     IAGQLEAPLALAPLARLEEQALAANPALAEARAAAEQARYRLRYEEAGRLPQVALRAT
                     READRELRQFKLGVSLTVPLWDRKSGPVHEAEAQRAQADLALGARAFAIRQQLEIAWR
                     QYEVAQTQVSALENGLVAQARSAVTVSESAYRAGERGLIDVLDAQRVYRSARADLIAS
                     RFELASAWVDIQRLTAAETIPPLNRKLP"
     gene            2123..3259
                     /locus_tag="VFF98_10615"
     CDS             2123..3259
                     /locus_tag="VFF98_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU64127.1"
                     /translation="MKKLCVLIAPLLLAACGEEAEKAVSAPPVDPALVVATPALAKRL
                     QVAQVAQAPVSDTLRVAGQIDFDENRVARIGATVTGRVAAMPANIGQHVGKGAVLAQI
                     VSTELSAQQLAYVRAKSAYELNRRNAERARALYAGDVISAAELQKRESEYQVSAAEMR
                     AAADQLRLLGLPGAALGQLGARGALGSASQVRATMSGVVVERHLALGQVVQPSDALFV
                     VADLSKVWAVAQVPEQQVRLVRLGEEVTLEIPALAGEKRKGRLVFIGHTVDPKTRTVL
                     IRTELDNASGALKPSMLATMLVAGEPEARLVVPAAAVVRENNADHVFVVERAGAYRLR
                     PVKLGEAHDGRRVVLSGLAAGEKIVVDGAFHLNTERNAQAVNTQ"
     gene            3256..>3720
                     /locus_tag="VFF98_10620"
     CDS             3256..>3720
                     /locus_tag="VFF98_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU64128.1"
                     /translation="MIQGLIRAALNQRLIVVVLALVLLGFGFNAARQLSVDAFPDVTN
                     VQVQIATEASGRSPEEVERMATAPIEVAMTGLPGLEEMRSLNKPGLSLITLVFSEQTN
                     VYFARQLVMERLSELGSKLTPGIAPVLGPVSTGLGEVYQYTLDKPGDGKAALG"
BASE COUNT          537 a         1237 c         1397 g          549 t
ORIGIN      
        1 ccgaactgcg cgcgctgatc ggcgccttca atgcgatgca gcagcggatc gacgggctgc
       61 tcgatgccaa tgcgcagacg atgctggcca tcggccatga tctgcgcacg ccgctggcgc
      121 gcctgcaatt gcggatcgac acgctgcgcc tgccggccga cgaccgcgcc gcgctggaag
      181 ccgatgtgga cgagatgcgc gatctgctgg gctccttgca ggcctatgtg gaaggcgatg
      241 gcgccggcca gcggcgcgag ccggtggatc tggcggcgat ggcgcagacg ctggtggacc
      301 aggccacgga gcagggggcc gaggccagct atgccggccc gccgcgcgtg gtggtggacg
      361 cccatgccac cgcgctgcgc cgggcgctgg ccaatttgct gggcaatgcg ctgcaatatg
      421 gcgggcgggc gcgggtgcgg ctgcgccagc agggcagcat gatcgagatc gccatcgagg
      481 acgacgggcc gggcattgcc gaggcgcatc tggccaaggt gctgcaaccc ttcgtgcggc
      541 tggaccatgc gcgtgaacgc aacacccgcg gcatggggct ggggctggcc atcgtcgata
      601 aggcggtgcg cgcgcaaggg ggcacgctgg tgctggaaaa ccggcccgcc ggcgggctgg
      661 tggcgcgcat ctgcctgccc gcgcgcggct gagccccttc agcaacactt ccttacaaac
      721 cggcaaatca cggaaagacg cgtccgtcta gcgtccctgc cagcttcggc gcggccgggg
      781 cgcttttcag gattcgggat ttcagcatga tggcatcggc cggttgcggc acaggcaggc
      841 ataaacgcgg gcggcgctgg ccgttggcgc tggcattctg gccgctggcg ctggcatggg
      901 caggcggggc ggcggcgggc gcaggcgggg cgggcgaggc ctattcgctg gccgcgctgg
      961 aagagctggc ccgcaccggc agcccggcgc tgcaggccgc cgccgccggc gtcgatggcg
     1021 cccgggcggg ggtggaaacg gcgcgcgcct tccccaatcc ggaagtggaa tatctggccg
     1081 gcaacacccg ctatcgcccc ggggtggatg gcgtgtcggg cgcggtgggc agcattggcc
     1141 tgtcgcagcc gctggatctg cccttccgcc gcacgccgcg cattgccgcc gcgcgcgcgg
     1201 ggctggccgc cgcccatgcc ggctatgccg ccatcgaggc ggactggatc gccgatctgc
     1261 gccgcgccta tttcgacatg ctgcgccgcg ccgccgaaac cgccaatgcc ggggaggatc
     1321 tggcgctgat gcaatcggtg caggcgaaga tcgcgctgcg ggtgcagaag ggcgatgcgc
     1381 cgcgtttcga gatgatccgc gccgaggcgg acctgctcaa cgtgcgcaag gcagcccagg
     1441 cggcagccct gcgcgaggag caggcgcggc tgcgcctgcg cgcgctggtg ggccctgatc
     1501 tgccggccga ttttgccatt gccggccagc tggaggcgcc gctggcgctg gccccgctgg
     1561 cgcggctgga agagcaggcg ctggcggcca atccggcgct ggccgaagcg cgcgcggcgg
     1621 ccgaacaggc gcgctatcgc ctgcgctatg aagaggccgg gcggctgccg caggtggccc
     1681 tgcgcgccac gcgcgaggcc gaccgcgagc tgcgccagtt caagctgggg gtcagcctca
     1741 ccgtgcccct gtgggacagg aagagcggcc ccgtgcacga ggccgaggcc cagcgcgccc
     1801 aggccgatct ggcgctgggc gcgcgcgcct ttgccatccg ccagcagctg gagatcgcct
     1861 ggcgccagta tgaagtggcc cagacccagg tgagcgcgct ggaaaacggg ctggtggcgc
     1921 aggcccgcag cgccgtcacc gtctccgaaa gcgcctatcg cgccggcgag cgcggcctca
     1981 tcgacgtgct cgatgcccag cgcgtctatc gctcggcccg cgccgatctc atcgccagcc
     2041 gcttcgagct ggccagcgcc tgggtcgata tccagcgcct caccgctgcc gaaaccatcc
     2101 cccctctcaa ccggaagctg ccatgaagaa actgtgtgtg ttgattgccc cgctgctgct
     2161 ggcggcctgc ggcgaagagg ccgaaaaggc ggtatcggcg ccgccggtgg atccggcgct
     2221 ggtggtggca acgccggcgc tggccaagcg cctgcaggtg gcacaggtgg cacaggcgcc
     2281 cgtgtccgac acgctgcggg tggccggcca gatcgatttc gacgagaacc gcgtggcccg
     2341 catcggcgcc acggtgaccg ggcgggtggc cgcgatgccg gccaatatcg gccagcatgt
     2401 gggcaagggg gcggtgctgg cgcagatcgt cagcaccgag ctgtcggccc agcagctggc
     2461 ctatgtgcgg gccaaatcgg cctatgagct gaaccgccgc aatgccgagc gcgcccgcgc
     2521 cctttatgcc ggcgatgtga tttccgccgc cgagctgcag aagcgcgaga gcgaatatca
     2581 ggtttccgcc gccgaaatgc gtgccgctgc cgatcagctg cggctgctgg gcctgccggg
     2641 cgcggcgctg gggcagctgg gcgcgcgcgg cgcgctgggc tctgcctcgc aggtgcgcgc
     2701 gacgatgagc ggcgtggtgg tggagcggca tctggcgctg ggccaggtgg tgcagccttc
     2761 cgatgcgctg ttcgtggtgg cagatctgtc gaaggtgtgg gcagtggccc aggtgcccga
     2821 acagcaggtg cggctggtgc gccttggcga ggaggtgacg ctggagatcc ccgcccttgc
     2881 cggcgagaag cgcaagggcc ggctggtgtt cattggccac acggtggacc ccaagacgcg
     2941 caccgtgctg atccgcaccg agctcgacaa tgccagcggc gcgctcaagc cttccatgct
     3001 ggccaccatg ctggtggcgg gcgagccgga agcccggctg gtggtgccgg ccgcggccgt
     3061 ggtgcgcgag aacaatgccg accatgtctt cgtggtggag cgggccgggg cctatcgcct
     3121 gcgcccggtg aagctgggcg aggcgcatga cgggcgccgg gtggtgctct cgggcctggc
     3181 ggcgggcgag aagatcgtcg tcgatggcgc cttccatctc aacaccgaac gcaacgcaca
     3241 ggcggtgaac acgcaatgat ccagggcctt atccgcgccg cgctcaatca gcggctgatc
     3301 gtggtggtgc tggcgctggt gctgctgggc tttggcttca acgccgcccg gcagctttcg
     3361 gtggatgcct tccccgatgt taccaacgtg caggtgcaga tcgccaccga ggcctcgggc
     3421 cgcagccccg aggaagtgga acgcatggcc accgccccca tcgaggtggc gatgaccggc
     3481 ctgcccggcc ttgaggagat gcgctcgctc aacaagccgg gcctctcgct catcacgctg
     3541 gtgttcagcg agcagaccaa tgtctatttc gcccgccagc tggtgatgga gcggctgagc
     3601 gagctgggct ccaagctgac gccgggcatt gccccggtgc tgggccctgt ttccaccggc
     3661 cttggcgagg tttaccagta tacgctggac aagcccggcg atggcaaggc cgcgcttggc
//
LOCUS       DASRYE010000207        13299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_256844,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000207 DASRYE010000000
VERSION     DASRYE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256844"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFF98_10625"
     CDS             <1..215
                     /locus_tag="VFF98_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HZU64129.1"
                     /translation="PWMDASLSSFSLVAQYWTARRHRANWLLWIVLDVIYVGMFLVKG
                     LYPTAGLYAGMTGLAVLGWRRWRAQG"
     gene            452..2791
                     /locus_tag="VFF98_10630"
     CDS             452..2791
                     /locus_tag="VFF98_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64130.1"
                     /translation="MTKHRSHQGLRRAMRSGLSVLSLAAALPFAPAVAHAGEAGDAAA
                     APAAAGTGDIVVTATRRSELAKDVPVAVTAIGGEKLDNLNSSGLDIRFLSGRTPSLQI
                     ESSFGRTFPRFYIRGLGNTDYDPNAAQPVSVVYDDVALESPMLKSFPVFDLKQVEVLR
                     GPQGSLFGRNTPAGVVKLESAKPTDYYTGYVSGEWGTYNTLNVEGAMSGPLGGGFTAR
                     ISGLLERRDNWVHNTNAGAAGSVAKSDLEGYRDVAGRLQIAYASPDKSFNALWDFHAR
                     DLAGTPRIFRAGMFQQGTNNFAAGFDPSKVSLDGYTSQQLTQWGTSLHLDYHIDGVGT
                     IYSISSYEKAKVESSGDIDGGATYTGSGLGVGAYQDSTGGYTRPEEISQEIRFASDEF
                     HGMRLQGGFYYFHQNLQYSEYEYDGNSGYTNRANDLDQDVEHYDVNENYGVFASGEAK
                     LGKLTLRAGVRYSEDHKNDLVWGWMPSYSIYSAYDSGNTITEPSGTYSVMTPQRMRVS
                     GSDVTWDASATYAATRAINAYARVATGYQGPAIQDRVTFGSYQSSAPKQTTISVEGGF
                     KGSALDHKLTFSIDAYYNETKNMQVTAVGGLTASAQLYSLKKVLGHGVEAEFELRPIE
                     GLSISASGSYNFTKIKDPNAYVSPCGGGCTVTNAIVDGLASINNNPLPQAPKWVGNIT
                     IGYERPVAPNIKAFVMTDWSYRSSVNFFLYEAKEFVGKPLTEGGVKIGVKTDDGWELA
                     FYGRNILNQIRAVGALDFNNLTGMINDPRILGVAVRKAW"
     gene            2901..3959
                     /locus_tag="VFF98_10635"
     CDS             2901..3959
                     /locus_tag="VFF98_10635"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HZU64131.1"
                     /translation="MALKPLVMALAAGLLAAAPARALPAKPLPIRVVVVTAFEVGADT
                     GDQPGEFQAWAAHLPQRIAAPALPRNLRYDPATGVLAISIGEGTGHAAASIMALGEDR
                     RFDLTHAYWLVAAIAGVNPHAASIGSAAWIGDIIDTDFSYYIDPREIPADWTTGFVPM
                     NRARPYAQPLPDSSDNLFALDKGLRDWAYALTRHVALPDNADLAAERAPYVGEPAALL
                     PPHVLVGAEASGQAFWLGEVMNRETEKWTAYWTGGQDRFVMTAMEDTGVARALAMLGR
                     EGRVDPRRLLVLRTASDYSTPPAGMAPAALLQKENRHLPGLQASLDAAYLVGHVVVDE
                     LVRHWSLYADHLPAVPAP"
     gene            3956..4144
                     /locus_tag="VFF98_10640"
     CDS             3956..4144
                     /locus_tag="VFF98_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64132.1"
                     /translation="MSAAADFAARARDEAAIAAAAAAQGLTLPAQCLPGIAANLAILA
                     DRARVLAEALAAEQEPGA"
     gene            4141..5463
                     /locus_tag="VFF98_10645"
     CDS             4141..5463
                     /locus_tag="VFF98_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HZU64133.1"
                     /translation="MSPLPKGIAATAAAVRKGQISALAVAEATLAVLRAGDGPIGAVV
                     TLLEARARADAARIDAALARGEDPGPLAGVPIGLKALFDVAGQPTTAGAAMRADAPPA
                     AADAELLARFTAAGAVPVAILNMDEYAYGFVTVNAHYGTTRNPRDTARLAGGSSGGSA
                     AAVAAGLLPLALGSDTNGSIRVPASLCGTYGFRPAFGSVPVAGSFPFVHCLDTLGPFT
                     ASLEDLRLAMEVSLARPLPAAEGALKVARLGGWFVDHAIDAFEEGFAAIAAVLGALPM
                     ADLPKVEAARAAAFLITGHDGGALHREELTRRPLAFDPEVRDRLLAGCLVPDALRAEA
                     GAFGDRFRASALALLEKYDVLLAPSVPDVAPLIADPFIHTGFGRAPARANLGLLAQPI
                     SLAGLPVLAAPLQRPDGLPLGLQLIGAPGCEGQLFALAARLEAAGVLA"
     gene            complement(5518..6639)
                     /locus_tag="VFF98_10650"
     CDS             complement(5518..6639)
                     /locus_tag="VFF98_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZU64134.1"
                     /translation="MPSLFRPLVALGLGLLAIAGPAAAETRPAPHAEPRKVIIDDDGF
                     ALMQAMLLKAPDVEVLGITTVSGDFWQNRATAMALRGLELVDRKEVPVSAGATFPLVN
                     SEALTDRWEALYGKLTWRGAWMHHWVEPTEQAEPEYHGPFDPVHLAWGDARLKPTGET
                     AAAFLIRMVHKYPHQITIVACGPMTNLALAQKLDPEFAGLTKELLYMGGSLNPHQVLE
                     GRAASDFAREFANSPRREFNFRFDPEAASIMSHSPFPKITAVPVDPSTATQLSPAFIA
                     RLKASAPAAMQPLFASLAPGFPLWDEIVAAVWLDPSLEKDGEDLYLDADTQFGPAYGD
                     MLSWREHYQPGLGEQKAHVVRSIDVPRFEALITQLVGRR"
     gene            complement(6716..7732)
                     /locus_tag="VFF98_10655"
     CDS             complement(6716..7732)
                     /locus_tag="VFF98_10655"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZU64135.1"
                     /translation="MTDLSALIAALPKAELHLHIEGSLEPELMFALARRNGIAIPYAS
                     VEDVRAAYSFTRLQDFLDIYYAGAAVLQTEQDFHDLAAAYFARAAADNIRHAEIFFDP
                     QTHTARGIPMATVIGGLRAAMAEAKARHGISSGLILCFLRHLSEEEALATLAEAEPFL
                     DQLLGVGLDSSEVGHPPEKFARVFARAGELGLKRVAHAGEEGPPAYVTGALDLLHIDR
                     LDHGNRALEDPALVARLAHSGMALTVCPLSNLKLCVVTDMADHPIPAMLEAGLCATVN
                     SDDPAYFGGYVNDNFRALDGAGLLTRARLLALVRNSFAGAFLPEAEKAAHLAEVERVA
                     AGFG"
     gene            complement(7729..9045)
                     /gene="guaD"
                     /locus_tag="VFF98_10660"
     CDS             complement(7729..9045)
                     /gene="guaD"
                     /locus_tag="VFF98_10660"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HZU64136.1"
                     /translation="MLTAYRAEILSLAEDPQVAGPAAICHHADGLLVVEGGHVVARGD
                     HAALAPRFPGLAVEDWRGHLLVPGFVDTHVHYPQTDRIASHGLQLLDWLSEHIFPAER
                     AFADAAHAREVADFFLAELLRNGTTTALVFATVHEGSVDALFAGAEARGMRMAAGKVL
                     MDLGPEGLADTPETARAESEALIRRWHGRGRLTYAVTPRFALTSSDAQLQVAGDLLAA
                     HPEALLHTHISENPREIEAVLGRFPAAADYLDVYDRFGLVGARSVFAHGIHLSHRECT
                     RLAEAGAGVALCPTSNLFLGSGLFDLARLDGHGVACGLGTDVGAGTSFSLLATMGEAY
                     KVCQLQGQALDPFRALYLATLGGARLLGLGDRIGALAAGQEADFLLLDPAATPLLARR
                     TRSATLTEKLFALQILGDDRAIAATYIAGRQAHCRKPAPSSLESCA"
     gene            complement(9039..10010)
                     /gene="xdhC"
                     /locus_tag="VFF98_10665"
     CDS             complement(9039..10010)
                     /gene="xdhC"
                     /locus_tag="VFF98_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HZU64137.1"
                     /translation="MGDLAALAPFAARAALGWAATARQALATGPVALVTVLATEGSAP
                     LGAGARMVVGQQGLLAGSVGGGALEWRALEQARAILAHAPGTWRVQDYPLGPLLGQCC
                     GGRVRLLVERLDPALAGWLAEALPGEALEAHYTPTAVRRHVVPAHKAPPGMADARSAK
                     PEAGAVLGEMLGALPRPVLLFGAGHVGQALERALAPLPFALHWFDTRPEFARPGVAIA
                     GEDTLIAAITQAAPEAALLILTHDHGLDYRLASAALARPQGAPLFTGVIGSATKAARF
                     RSRLGAQGLAAARLTCPIGLAGIGGKAPAVIAASVAAQLLMLESAPC"
     gene            complement(10003..12351)
                     /gene="xdhB"
                     /locus_tag="VFF98_10670"
     CDS             complement(10003..12351)
                     /gene="xdhB"
                     /locus_tag="VFF98_10670"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HZU64138.1"
                     /translation="MAEADPKMGDTFLVHRPTPHDSAAMQVAGSALYIDDLPEPPGLL
                     HCAFGLATEAHAEILAMDLDAVRHAPGVVAVFTAADIPGRNDVSPFAGDDPMLAEGLV
                     HFLGQPLFLVAATSARAARRAARLGRISYAPRPALLTIEEALAAGSFIEPSQTMGHGD
                     VPAALAAAPHRLEGRLEIGGQDHFYLEGQIALATPGEDGTIHLAASSQHPSEVQHLVA
                     HLLHRHSADVVVEVRRMGGGFGGKETQPALFAAAAALVAAKTGRPAKFRADRDDDMAI
                     TGKRHDFRILYEVGCDAAGRVLGIRYDLAARCGWSTDLSPAICDRAMFHADNCYFLPA
                     VEIISHRCRTNTVSNTAFRGFGGPQGMIAAERVMDHIAAHLGLDPLAVRRANLYGLPG
                     AQGAPHATRRLTPYGMEVADNIAPDIIAELEASADYAARRQAVAAFNAASPVLKKGLS
                     LTPVKFGISFTTTHLNQAGALVLVYADGSVALNHGGTEMGQGLNIKVAQVVADVFGIS
                     LDTIRITATRTDKVPNTSATAASSGSDLNGMAAWDAAMQIRTRLAALVAEAAGVAPEA
                     VRFTPAGVVAGALTLPFAEVARRAYLARVQLSASGFYATPEIHYDRPTHTGRPFYYFA
                     YGAALSEVVVDTLTGEHRVLRADILHDAGRSLNPAIDLGQVEGGFIQGMGWLTTEELV
                     FSAKGSLLTHAPSTYKIPTANDRPPVMEIRLWPHGRAHVPTIHRSKAVGEPPFMLANS
                     VFGALTQAVMAAAPGKGLPLLDAPATPERVLAALAELRARHG"
     gene            complement(12344..>13299)
                     /locus_tag="VFF98_10675"
     CDS             complement(12344..>13299)
                     /locus_tag="VFF98_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZU64139.1"
                     /translation="LAALARPHMLELTHEDALAGQTRRAFLPRSADELAEVLLAHPDA
                     RIVAGATDVGLWVTKQQRDIPVLVFLGDCADLKGIEDSADGLTLYAGVRYSEAQAALA
                     RLHPELGELVRRIAGTQVRNAGTICGNIANGSPIGDTPPALIALDARITLRHGQARRE
                     VALADFFIAYGKQDRRPGEWVESLFIPRPAADLKLRIAKLSKRFDSDISAVCAAFALQ
                     VEAGQVSFARIAFGGMAATPKRARAAEAALLGQPWCEASVEAACAALATDYQPLNDVR
                     GSAAYRLAAAANLLRRLWVEEAHPGAALSVLDPALVEAHHG"
BASE COUNT         2102 a         4549 c         4613 g         2035 t
ORIGIN      
        1 tgccgtggat ggatgccagc ctctccagct tcagcctcgt tgcccaatat tggacagccc
       61 gccgccaccg cgccaactgg ctgttgtgga tcgtgctgga tgtcatctat gtcggcatgt
      121 tcctggtgaa ggggctctat cccaccgcgg gcctgtatgc cgggatgacc ggccttgccg
      181 tgctgggctg gcggcgctgg cgcgcacaag gctgaaccgc aggcttgccc ctgtggcata
      241 tacggataag cctgttgcat aaaggccacg gcttttggcg aaaccgcaca gggcgttctt
      301 attattagca cgctgcggtg cctgtagaga cttttttatc acaagccccc ttgtgggccg
      361 ccgcgcctct ggtaggatgg caccgtaatg aacccgcgtc agacgggcat tcgggaaggg
      421 ggcataacat ccatcacctc agggggttga tatgaccaag caccgctctc accagggcct
      481 tcggcgcgcc atgcgctcgg gcctttccgt tctgtccctt gctgccgcgc tgccttttgc
      541 gccggctgtg gcccatgccg gcgaggcggg cgatgccgcc gctgcccctg cggccgcggg
      601 cacgggcgat atcgtcgtca ccgccacccg ccgctccgag ctggccaagg acgtgccggt
      661 ggccgtcacc gcgatcggcg gcgaaaagct cgacaatctg aattccagcg gccttgatat
      721 ccgcttcctc tcgggccgca cgccttcgct gcagatcgaa agctcgttcg gccgcacctt
      781 cccgcgcttc tacatccgcg gccttggcaa caccgattat gaccccaacg ccgcccagcc
      841 ggtgtcggtc gtctatgacg atgtggcgct ggaaagcccg atgctgaaga gcttcccggt
      901 gttcgacctc aagcaggtgg aagtgctgcg cgggccgcag ggctcgctgt tcggccgcaa
      961 cacgccggcc ggtgtggtga agctggaatc ggccaagccg accgattatt acaccggcta
     1021 tgtctcgggc gaatggggca cctacaacac gctgaacgtt gaaggggcga tgtctggccc
     1081 gctgggcggc ggcttcacgg cgcgcatttc gggcctgctg gaacggcgcg acaactgggt
     1141 gcacaacacc aatgccggcg ctgcgggctc ggtcgccaag tcggatctgg aaggctatcg
     1201 cgatgtcgcc ggccgcctgc agatcgccta tgcctcgccc gacaagtcgt tcaacgcgct
     1261 gtgggatttc cacgcgcgtg atctggccgg cacgccgcgc atcttccgcg ccggcatgtt
     1321 ccagcagggc accaacaatt ttgccgccgg ttttgatccc tcgaaggtca gcctcgatgg
     1381 ctacaccagc cagcagctga cccagtgggg caccagcctc cacctcgatt accatatcga
     1441 tggcgtgggc acgatctact cgatctcctc ctacgagaag gccaaggtcg aaagctcggg
     1501 cgatatcgat ggcggcgcca cctacaccgg ctctggcctg ggcgtgggcg cctatcagga
     1561 ttccaccggc ggctacacgc ggcccgagga aatcagccag gaaatccgct tcgcctcgga
     1621 tgagttccac ggcatgcggc tgcagggtgg cttctattat ttccaccaga acctgcagta
     1681 cagcgaatat gaatatgacg gcaacagcgg ctacaccaac cgcgccaacg atctcgacca
     1741 ggatgtcgaa cattacgacg tcaacgagaa ctatggcgtg tttgccagcg gcgaggccaa
     1801 gctgggcaag ctgacgctgc gcgccggcgt gcgctattcg gaagaccaca agaacgatct
     1861 ggtgtggggc tggatgccct cctactcgat ctattccgcc tatgattcgg gcaacaccat
     1921 caccgagccc tcgggcacct acagcgtgat gaccccgcag cgcatgcgcg tgtcgggctc
     1981 tgacgtgacc tgggatgcca gcgccaccta tgccgccacc cgcgcgatca acgcctatgc
     2041 gcgcgttgcc accggctatc agggcccggc catccaggac cgcgtgacct tcggcagcta
     2101 tcagagctct gcccccaagc agacgacgat ctcggtggaa ggcggcttca agggctcggc
     2161 gctggatcac aagctgacct tctcgatcga tgcctactac aacgagacaa agaacatgca
     2221 ggtgacggcg gtcggcggcc tcaccgcctc ggcccagctc tacagcctga agaaggtgct
     2281 cggccatggt gtcgaggccg aattcgagct gcgcccgatc gagggcctgt cgatctcggc
     2341 ctcgggcagc tacaacttca ccaagatcaa ggatcccaac gcctatgtct cgccctgcgg
     2401 cggcggctgc acggtgacca acgccatcgt cgatggcctg gcctcgatca acaacaaccc
     2461 gctgccccag gcgcccaagt gggtgggcaa catcaccatc ggctatgaac ggccggtggc
     2521 gcccaacatc aaggccttcg tgatgaccga ctggtcctat cgctccagcg tcaacttctt
     2581 cctctacgag gcgaaggaat tcgtcggcaa gccgctgacc gaaggcggcg tgaagatcgg
     2641 cgtgaagacg gatgatggct gggaactggc cttctatggc cgcaacatcc tcaaccagat
     2701 ccgcgccgtg ggcgcgctgg acttcaacaa cctgaccggc atgatcaacg atcccaggat
     2761 cctgggcgtg gccgtgcgca aggcctggta agcaggcaac agggcagggg gcgcggcgat
     2821 ggctgcgccc cctgccttgc ccgcgatggt gcgggcccat cggccgcgcc gcacaggccc
     2881 gcgccaaagg acaggatata atggccctca aacctctcgt catggcgctg gccgctggcc
     2941 tgctggccgc cgcgcctgcc cgcgccctgc cggccaagcc gctgccgatc cgcgtggtgg
     3001 tcgtcaccgc tttcgaggtg ggggcggaca cgggcgacca gcccggcgaa ttccaggcct
     3061 gggcggccca tctgccgcag cgcatcgccg cgcccgccct gccgcgcaat ctgcgctatg
     3121 atccggccac cggcgtgctg gcgatcagca tcggcgaggg caccggccat gccgccgcct
     3181 cgatcatggc gctgggcgag gaccgccgct tcgatctcac ccatgcctat tggctggtgg
     3241 ccgcgattgc cggggtgaac ccgcatgccg cctcgatcgg ctctgccgct tggattggcg
     3301 acatcatcga caccgatttt tcctactaca tcgacccgcg cgaaatcccc gccgactgga
     3361 ccacaggctt cgtgccgatg aaccgcgcgc gcccctatgc ccagccgctg cccgacagca
     3421 gcgacaatct cttcgcgctg gacaagggcc tgcgcgactg ggcctatgcg ctcacccgcc
     3481 atgtcgccct gcccgacaat gccgatctgg ccgccgagcg cgcgccctat gtgggcgagc
     3541 ctgccgcgct gctgccgccc catgtgctgg tgggcgccga agccagcggc caggccttct
     3601 ggctgggcga ggtgatgaac cgcgagaccg agaagtggac cgcctactgg acaggcggcc
     3661 aggaccgctt cgtgatgacc gccatggaag acacaggcgt tgcccgcgcg ctggccatgc
     3721 tgggccgcga ggggcgggtt gatccgcgcc ggctgctggt cttgcgcacc gccagcgatt
     3781 attccacccc gcctgcgggc atggccccgg ccgccctgct gcaaaaggag aaccgccact
     3841 tgcccggact tcaggcctcg ctcgatgcgg cctatcttgt tggccatgtc gtggtcgacg
     3901 agctggtgcg ccactggtcg ctttatgccg atcatctgcc cgcggtgccc gcgccatgag
     3961 cgccgccgcc gattttgccg cccgcgcccg ggatgaggcg gcgattgccg ccgccgctgc
     4021 cgcgcagggg ctgacgctgc cggcgcaatg cctgccgggc atcgccgcca accttgccat
     4081 cctggccgat cgcgcccgcg tgctggcaga ggcgctggcc gcagagcagg agccgggcgc
     4141 atgagcccgc tgcccaaggg cattgccgcc accgccgctg ccgtgcgcaa aggccagatc
     4201 agcgcgctgg ccgtggcaga ggcaacgctg gccgtcctgc gtgctggcga tggcccgatc
     4261 ggcgcggttg tcaccctgct ggaagcgcgc gcgcgggccg atgccgcccg catcgatgca
     4321 gcgctggccc ggggcgagga tccgggcccg ctggccggcg tgccgatcgg cctcaaggcg
     4381 ctgttcgacg tggccggcca gcccaccacc gctggcgctg ccatgcgcgc cgatgcgccg
     4441 ccggccgctg ccgatgccga gctgctggcc cgcttcacgg cggcaggcgc ggtgcccgtc
     4501 gccatcctca acatggacga atacgcctat ggcttcgtca cggtgaacgc ccattacggc
     4561 accacccgca acccgcgcga cacggcgcgg ctggcgggcg gttcctcggg cggatcggcc
     4621 gcagcggtgg ccgccggcct gttgccgctg gcgctggggt cagacaccaa cggctcgatc
     4681 cgcgtgcccg cctcgctgtg cggcacctat ggcttccgcc cggcctttgg cagcgtgccg
     4741 gtggccggca gcttcccctt cgtccattgc ctcgacacgc tgggcccctt caccgccagc
     4801 ctggaagacc tgcggctggc gatggaagtg agccttgcgc ggcccctgcc ggcggccgag
     4861 ggcgcgctga aagtggcgcg gctgggcggc tggttcgtcg atcatgcgat cgatgcgttt
     4921 gaagagggct ttgccgccat tgccgctgtg ctgggcgccc tgccgatggc cgatctgccc
     4981 aaggtggaag cggcccgggc ggcggccttc ctcatcaccg gccatgatgg cggcgcgctg
     5041 cacagggaag agctgacccg ccgcccgctc gccttcgatc cggaagtgcg cgatcggctg
     5101 ctggccggct gcctggtgcc cgatgccctg cgggcagagg ccggcgcttt tggcgatcgc
     5161 ttccgcgcca gcgccctggc gctgctggag aagtatgacg tgctgctggc cccctcggtg
     5221 cccgatgtgg cgccgctgat tgccgatccg ttcatccaca caggctttgg ccgggcgccg
     5281 gcgcgggcca atctgggctt gctggcccag ccgatcagcc tggccggcct gccggtgctg
     5341 gccgccccgc tgcagcgccc ggacggcctg ccgctgggcc tgcagctgat cggcgcgccg
     5401 ggctgcgagg ggcagttgtt cgcgctggcc gcgcggctgg aagcggccgg cgtgctggcc
     5461 tgaaaagggc cgctgcgagg gggcgcgccc ctcgcagcgg ccctcgcagc gttggcctca
     5521 gcgccgcccc accagctggg tgatcagcgc ctcgaagcgg ggcacatcga tgctgcgcac
     5581 cacatgggcc ttctgctcgc caaggccggg ctgatagtgt tcgcgccagc tcagcatgtc
     5641 gccataggcc gggccgaact gggtgtcggc atcaaggtag aggtcctcgc catccttctc
     5701 cagcgaggga tccagccaga ccgccgcgac gatctcgtcc cacagcggga agcccggcgc
     5761 caggctggcg aacagcggct gcatggccgc cggcgcgctg gccttcagcc gggcgatgaa
     5821 agcgggcgac agctgcgtgg cggtggacgg atcgaccggc acggcggtga tcttcgggaa
     5881 ggggctgtgg ctcatgatgc tggcggcttc ggggtcgaag cggaagttga actcgcgccg
     5941 gggggaattg gcgaattcac gcgcgaaatc gctggccgcg cgaccttcca gcacctgatg
     6001 cgggttcagg ctgccgccca tgtagagcag ctctttcgtc agaccggcaa attcggggtc
     6061 cagcttctgg gccagcgcca gattggtcat cggcccgcag gcaacgatgg tgatctggtg
     6121 cggatatttg tgcaccatgc ggatgaggaa ggcggccgcc gtctcgccgg tgggcttcag
     6181 ccgggcatcg ccccaggcca gatgcaccgg atcgaagggg ccgtgatatt ccggctctgc
     6241 ctgctcggtc ggctccaccc agtggtgcat ccacgccccg cgccaggtca gcttgccata
     6301 cagcgcttcc cagcggtcgg tcagcgcctc ggaattgacc agcgggaagg tggccccggc
     6361 cgacaccggc acctccttgc ggtccaccag ctccagcccg cgcagggcca tggcggtggc
     6421 gcggttctgc cagaaatcgc ccgagaccgt ggtgatgccc agcacctcca catcgggcgc
     6481 cttcagcagc atggcctgca tcagggcaaa gccgtcgtcg tcgatgatga ctttacgggg
     6541 ctcggcatgg ggggcgggcc gggtttcggc agcggcaggg ccggcgatgg ccagcaggcc
     6601 aaggccaagg gcaaccagcg ggcggaagag ggagggcatg ggcgttgatc cttggtgtgt
     6661 cgtgtcagcc ctctcccgtt tgtggttgtg tgtttggggc cgatcgcggg gccgctcagc
     6721 caaagccggc ggccacgcgc tccacttcgg caagatgggc ggccttttcg gcctcgggca
     6781 ggaaggcgcc ggcaaaggaa ttgcgcacca gcgccagcag gcgcgcgcgg gtgagcaggc
     6841 cggcgccatc cagcgcgcgg aaattgtcgt tcacatagcc gccgaaatag gccggatcgt
     6901 cggaattgac ggtggcgcac aggcctgctt ccagcatcgc ggggatgggg tgatcggcca
     6961 tgtctgtcac cacgcacagc ttgaggttcg acagcgggca gacggtgagc gccatgccgc
     7021 tgtgcgccag gcgcgccacc agcgccggat cttccagcgc gcggttgcca tggtccagcc
     7081 ggtcgatatg cagcagatcg agcgcgccgg tcacataggc cggcgggcct tcctcgccgg
     7141 catgggccac gcgcttcagc cccaactcgc ccgcccgggc aaacacgcgg gcgaattttt
     7201 ccggcggatg gccgacttcc gatgaatcga gcccgacccc cagcagctgg tcgaggaacg
     7261 gctcggcctc ggccagggtg gccagcgcct cttcctcgga aaggtggcgc aggaagcaca
     7321 ggatgaggcc cgagctgatg ccatggcggg cctttgcctc ggccatcgcc gcgcgcagcc
     7381 cgccgatcac cgtggccatg ggaatgccgc gcgcggtatg cgtctgcggg tcgaagaaga
     7441 tctcggcgtg gcggatattg tcggccgcgg cgcgggcgaa ataggcggcg gccaaatcgt
     7501 ggaaatcctg ctcggtctgc aacacggcgg cgccggcgta gtagatgtcg aggaaatcct
     7561 gcagccgggt gaagctgtag gcggcgcgca catcctccac gctggcatag gggatggcga
     7621 tgccattgcg ccgggccagg gcaaacatca gctcgggctc gaggctgccc tcgatatgca
     7681 ggtgcagctc tgccttgggc agggcggcga tgagggcgga aaggtcggtc atgcgcagct
     7741 ttcgagcgag gagggggcgg gcttgcggca atgggcttgc cggccggcga tataggtggc
     7801 cgcgatcgcc cggtcatccc ccaggatctg cagggcgaac agcttttcgg tcagggtggc
     7861 gctgcgggtg cggcgggcca gcagcggcgt tgcggccgga tccagcagca ggaaatcggc
     7921 ctcttgccct gctgccagcg cgccgatcct gtcacccagg cccagcagcc gcgcgccgcc
     7981 cagcgttgcc agataaagcg cgcggaaggg atcgagcgcc tgcccttgca gctggcacac
     8041 cttgtaggcc tcgcccatcg tcgccagcag cgagaagctg gtgcccgccc ccacatcggt
     8101 gcccagcccg caggccacgc catggccatc cagccgcgcc agatcgaaca ggccagagcc
     8161 gaggaagagg ttcgatgtgg ggcacagggc cacgcccgcg cccgcttctg ccagccgcgt
     8221 gcattcgcgg tgcgacaggt ggatgccatg ggcaaacacc gaacgcgcgc cgacgaggcc
     8281 aaagcggtca tacacgtcga gataatccgc cgctgcggga aagcggccca gcacggcctc
     8341 gatctcgcgc ggattttccg agatgtgggt gtggagcagc gcttccgggt gggccgccag
     8401 cagatcgccc gctacctgca attgcgcatc cgaggaggtg agcgcgaaac gcggggtgac
     8461 ggcataggtg agccggccgc gcccgtgcca gcggcggatc agcgcctcgc tctcggcgcg
     8521 cgcggtttcg ggcgtgtcgg ccaggccttc ggggcccagg tccatcagca ccttgccggc
     8581 ggccatgcgc atgccgcgcg cctccgctcc ggcaaacagc gcatccacgc tgccttcatg
     8641 caccgtggcg aagaccagcg ccgtggtggt gccattgcgc agcagttcag cgaggaagaa
     8701 atcggccact tcgcgggcat gggcggcatc ggcaaaggcg cgctcggccg ggaagatgtg
     8761 ttccgacagc cagtcgagca gttgcagccc gtgcgaggcg atgcggtctg tctgcggata
     8821 atggacatgg gtgtcgacaa agccgggcac cagcagatgg ccgcgccaat cctccaccgc
     8881 aaggccgggg aagcgcggcg ccagcgcggc atgatcgccg cgcgccacca catggccgcc
     8941 ttccaccacc agcagcccat cggcatggtg gcagatggcg gccgggcccg ccacctgcgg
     9001 atcctcggcc aggctgagga tctcggcgcg ataggctgtc agcatggcgc gctctccaac
     9061 atcagcaatt gcgcggccac gctggcggcg ataaccgccg gcgccttgcc gccaatgccg
     9121 gcaaggccga tcgggcaggt gaggcgggcg gctgccagcc cctgcgcccc aaggcgcgag
     9181 cggaagcggg cggccttggt ggcagagccg atcacgccgg tgaacagcgg cgccccctgc
     9241 gggcgggcaa gggcggcgct ggcgaggcga tagtcgaggc catggtcatg cgtcaggatc
     9301 agcagggcgg cctcgggggc ggcctgggta atggcggcga tcagcgtatc ttctccggca
     9361 atggcaacgc ccgggcgggc aaattcgggc cgcgtgtcga accagtggag ggcaaagggc
     9421 agcggcgcca gcgcgcgttc cagcgcctgg cccacatggc cggcgccaaa cagcagcacc
     9481 ggccgcggca gcgcgcccag catctcgccc agcacggcgc cggcttcggg tttggcgctg
     9541 cgggcatcgg ccatgccggg cggcgccttg tgagcgggca ccacatggcg gcgcacggcc
     9601 gtgggcgtgt aatgggcttc cagcgcctcg ccgggcaggg cttcggccag ccagccggcc
     9661 agcgccgggt ccagccgctc caccagcagg cgcacgcgcc cgccgcagca ctggcccagc
     9721 aacgggccca gcggataatc ctgcacccgc cacgtgcccg gcgcatgggc aaggatggcg
     9781 cgcgcctgtt ccagcgcccg ccattccagc gcgccgccgc ccacagagcc cgccagcagc
     9841 ccttgctgcc ccaccaccat gcgcgcgccc gcgcccagcg gggcagagcc ttcggtggcc
     9901 agcaccgtga ccagcgccac cggccctgtg gccagcgcct ggcgcgcggt ggccgcccag
     9961 cccagcgcgg cgcgggcggc aaagggggca agggcggcca gatcacccat ggcgggcgcg
    10021 cagttcggcc agcgccgcca gcacgcgttc gggcgtggcc ggcgcatcga gcaggggcag
    10081 gcccttgccg ggggcggctg ccatcaccgc ctgcgtcaac gcgccgaaca cgctgttggc
    10141 cagcatgaag ggcggctcgc ccaccgcctt ggaacggtgg atggtgggca catgggcgcg
    10201 gccatgcggc cacaggcgga tctccatcac cggcgggcga tcattggcgg tggggatctt
    10261 gtaggtggaa ggggcatgcg tcagcaggct gcccttggcc gagaacacca gctcctcggt
    10321 ggtcagccag cccatgccct ggatgaagcc gccttccacc tggcccagat cgatcgccgg
    10381 gttcagcgag cggccggcat catgcaggat gtcggcgcgc agcacccggt gctcgccggt
    10441 cagcgtgtcc accaccactt cgctcagcgc cgcgccatag gcgaaatagt agaacgggcg
    10501 gcccgtgtgg gtggggcggt cgtagtggat ctcgggcgtg gcgtaaaagc cgctggcgga
    10561 aagctgcacg cgggcgagat aggcccgccg cgccacttcg gcaaagggca gggtgagggc
    10621 gccggccacc acccctgccg gcgtgaagcg caccgcctcg ggcgccacgc ccgccgcttc
    10681 tgccaccagc gctgccagcc gggtgcggat ctgcatggcc gcatcccagg cggccatgcc
    10741 attgaggtcg ctgcccgagg aagcggcggt ggcactggtg ttgggcacct tgtcggtgcg
    10801 ggtggcggtg atgcggatgg tatcgagcga tatgccgaaa acatcggcca ccacttgcgc
    10861 caccttgatg ttcagcccct ggcccatctc cgtgccgcca tggttcagcg ccacagagcc
    10921 gtcggcatag accagcacca gcgcgccggc ctggttgaga tgggtggtgg tgaaggaaat
    10981 gccgaatttg accggggtga gcgacagccc cttcttcagc accgggctgg ccgcgttgaa
    11041 ggcggccacg gcttgccggc gggcggcata atcggcgctg gcttccagct cggcgatgat
    11101 gtcgggggcg atgttgtcgg ccacctccat gccatagggg gtgaggcggc gggtggcatg
    11161 gggcgcccct tgcgcgcccg gcaggccata gaggttggcc cgccgcaccg ccagcggatc
    11221 cagccccaga tgggcggcga tatggtccat cacccgctcg gccgcgatca tcccctgcgg
    11281 gccgccaaag ccgcgaaacg ccgtgttcga cacggtgttg gtgcggcagc ggtggctgat
    11341 gatctccacc gccggcagga agtagcaatt gtcggcatgg aacatcgccc ggtcgcagat
    11401 ggccggcgac agatcggtgg accagccgca gcgcgcggcc agatcatagc ggatgcccag
    11461 aacgcgcccg gccgcatcgc agcccacctc gtagaggatg cggaaatcat gccgcttgcc
    11521 ggtgatcgcc atgtcatcgt cgcggtcggc gcggaatttg gcggggcggc cggttttggc
    11581 cgccaccagc gcggcggcgg cggcaaacag ggcgggctgg gtttccttgc cgccaaagcc
    11641 gccgcccatg cggcgcactt ccaccaccac atcggcgctg tggcgatgca gcagatgcgc
    11701 caccagatgc tgcacttcgg aagggtgctg gctggaggcg gcaaggtgga tggtgccatc
    11761 ctcgcccggg gttgccagcg cgatctgccc ttcgaggtag aaatgatcct gcccgccgat
    11821 ctccagccgg ccttccagcc ggtgcggggc ggcggccagg gctgcgggca catcgccatg
    11881 gcccatcgtc tggctgggct cgatgaagct gccggcggcc agcgcttcct cgatggtgag
    11941 aagcgccggg cgcggcgcat agctgatgcg ccccagcctg gccgcccgcc gcgccgcccg
    12001 cgcgctggtg gcggccacca gaaacagcgg ctggccaagg aaatgcacca gcccttcggc
    12061 cagcatcgga tcatcgccgg caaagggcga gacatcgttg cgcccgggaa tatcggccgc
    12121 ggtgaacacc gccaccacgc cgggggcgtg gcgcacggca tccagatcca tcgccaggat
    12181 ctcggcatgg gcttcggtgg caaggccaaa ggcgcagtgc agcaggcccg gcggctcggg
    12241 caggtcgtcg atatagagcg cgctgccggc cacctgcatg gcggccgaat catgcggcgt
    12301 ggggcggtgg acgaggaaag tgtcgcccat tttcggatcg gcctcagcca tgatgcgcct
    12361 ccaccagcgc gggatcgagc acggagaggg ccgcgccggg atgcgcctcc tccacccaca
    12421 ggcggcgcaa cagattggcc gctgccgcca gccgataggc cgcactgccg cgcacatcgt
    12481 tcaagggttg gtaatctgtg gcaagggccg cgcaggccgc ttccacgctg gcctcgcacc
    12541 agggctggcc cagcagtgcg gcctcggcgg cgcgggcgcg tttgggggtg gccgccatgc
    12601 cgccaaaggc gatgcgcgcg aaggacacct ggccggcttc cacctgcagc gcaaaggcgg
    12661 cgcaaacggc cgagatgtcg ctgtcgaacc gcttggaaag cttggcgatg cgcagcttca
    12721 gatctgccgc cgggcggggg atgaacaggc tttccaccca ttcgccgggg cggcgatcct
    12781 gcttgccata ggcaatgaag aaatccgcca gcgccacttc gcgccgggct tgcccgtgcc
    12841 gcagcgtaat gcgcgcatcc agcgcgatca gcgccggcgg cgtgtcacca atgggcgagc
    12901 cattggcgat attgccgcag atggtgccgg cattgcgcac ctgcgtgccg gcaatgcggc
    12961 gcaccagctc gcccagttcg gggtgcaggc gggccagcgc tgcctgcgcc tcgctgtagc
    13021 gcacgccggc ataaagggtg aggccatcgg cgctgtcctc gattcccttc agatccgcgc
    13081 aatcgcccag gaacaccagc accggaatat cgcgctgctg cttggtcacc cacaggccga
    13141 catcggtggc gccggcaacg atccgcgcat cgggatgggc cagcagcacc tcggccagct
    13201 catcggcaga gcgcggcagg aaggcgcggc gggtctgccc ggccaacgcg tcctcgtggg
    13261 tcagctccag catgtggggg cgggcaaggg cggcaagct
//
LOCUS       DASRYE010000208         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_135881,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000208 DASRYE010000000
VERSION     DASRYE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135881"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFF98_10680"
     CDS             <1..1049
                     /locus_tag="VFF98_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64140.1"
                     /translation="SALTPAAASLPAQINAAMAQSAIRAAPYRRARRAIVRAIGQIGD
                     RRLAAATRAALLQPGDCLRHRTGLSRADEEAIVARLRTAGFLTPELAPEAAREGLFPP
                     VLAAGSACPHLAVPVLAAAGGNSRSHHSWPGGLAIHTAENLALGSRLARAYPGLPQPA
                     QDGWRAAILWHDWAKALVLPWQADGLTRPELQIAGTGAHHILGLAEAMRRGLPGPVIA
                     AQACAHALPGTDGAPVAGWLQAAAIIARRPTTQVPTARPGPACRITFAADQSWPRTDA
                     ALARAEVRLRALAQPCGRDSGPAFEIGVKNALLARQGTALKAGRSLRPCPSTRATPEP
                     HGYLPPTPLSSFGA"
     gene            1105..>5796
                     /locus_tag="VFF98_10685"
     CDS             1105..>5796
                     /locus_tag="VFF98_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HZU64141.1"
                     /translation="MPETSHAPLPPADTLSAALAATLAARLGTAFLGEEETPEPEALA
                     QAAHFLREAAASRAPGLPALAIEPFLTATGQRLTRIAVINDDMPFLVDTIAGAVSTEG
                     LAIERLAHPVLAAERDGAGRLAALPAGDVPGTARESFVYLETARANARTRGRLATGIQ
                     RALADGRAAVADWEAMRAAMAADAATLARQDRSEAAALLRWLAADMFTLLGHVRHTRD
                     GGASAALGICRASTPAPLPPEALAEAFAWCDAFMAGGGGRLPLIVKSDHLALVHRRVP
                     LDLFIVPEIAGGRVEALSIHAGLWTSAALASPPAQVPLMRAQLATLMGKFGFASDGHA
                     GKVLAHALTSLPHDQLIAFSDDDLERVALAMMSLLDRPRPRLALVAAPMARQLFAFIW
                     LPRDMFSTDVREAITRLLVEATGARVLDWSLEVESSGLAQLRFVLARSDHAAPGAPAA
                     PAPAGTAPARAPADEAALDAQLGAMLRGWDEAVEAELAHSLPPRRAAAVAARYAGAFP
                     PAYRSLYGPAQAAIDIGHLRHLVARKGQALRRAVRLYALASDPPERLRLKIYQATGAL
                     VLSEVVPALENFGFRVIDTMPTHLAMASHGQAPAQGGLGTIHDILLALPAPAPDAPAS
                     HPADSARRAVLHHAPIVEDALGAMLNGAGEDDPFNRLIVGVGLSAREANWLRALYRYL
                     RQAGMSQGVDTAVDALMRAPVVALALVDLLRARHQPGIDGRAAAEALAEATLSAGLGE
                     VAAIQDDRLLRAFGAAVRALLRTNAFSPASTQALAFKFDSALVPGLPRPLPWREIFVY
                     APEVEGIHLRAGPVARGGLRWSDRRDDFRTEVLGLMKAQRVKNAVIVPTGAKGGFFPK
                     HLPDPATHRAAWAEGGRAAYQTFVRALLSLTDNLVDGQLVHPAGITIRDGADPYFVVA
                     ADKGTATFSDTANAIAAEAGFWLGDAFASGGSVGYDHKAMGITARGAWVSVQRHFREM
                     GVDVQAEPVRVAGVGDMSGDVFGNGMLLSKAICLVAAFDHRHIFLDPAPDPATAWAER
                     ARLFALPRSSWADYDSALISKGGGVFARSLKSIPLSPEARAALGTEAETIEPDALIRA
                     ILTASVDLLWFGGIGTYVKAASQSDAEAGDRTNDALRVNGAQLRARVVGEGANLGVTQ
                     AGRIEYALAGGRINTDFIDNSAGVDCSDKEVNIKIALASARRAGRLAEADRPALLAGM
                     TDAVAELVLDDNRQQALGLSVAQGGGVAAVPAYLRLIDQLEEAGQLDRATEGLADAET
                     FTRRAAGHDDGQDGRGLTRPELAVLLSSAKLALQAALEAAPLLDDPELTPLFAPLLAA
                     AFPEAMRRGFAADIAGHQLRREILATGIANRVVNRLGLLAPFELAEEEGVGLADVAAA
                     CLAAEALFGMAPLWRAIDTGVMPEAARIALMGAAAAGLRGHMSELLRAEPAAPMALAS
                     ALGPTIAELADERESQQEPAARAQSAARLAALTDAGAPVDLAISVNRLADMAGAVGLA
                     YLAHGRRCHLPALAQAFARLGTALDLDWAEAAAARLAPADPWERLLAGGLACDFQQMR
                     LARLARLAEGDP"
BASE COUNT          750 a         2207 c         2023 g          816 t
ORIGIN      
        1 ccagcgccct gacgccagcc gctgccagcc tgcccgcgca gatcaacgcc gccatggccc
       61 agagcgcgat ccgcgcggcc ccctatcggc gggcgcggcg ggccattgtg cgcgccatcg
      121 gccagatcgg cgatcgccgg ctggcggcgg ccacccgcgc ggccctgctg caaccgggcg
      181 attgcctgcg ccaccgcacc ggcctgtccc gcgcggatga ggaagccatt gtcgcccgcc
      241 tgcgcaccgc cggcttcctc accccggaac tggcgccaga agcggcgcgc gagggcctgt
      301 tcccccccgt gctggccgcg ggcagcgcct gcccgcacct cgccgtgccg gtgctggcgg
      361 cggccggcgg caacagccgc tcgcaccaca gctggccggg cgggctggcc atccacacgg
      421 cggaaaacct ggcgctgggc agccggctgg cgcgggccta tccgggcctg ccacagccgg
      481 cgcaggatgg ctggcgcgcg gccatcctgt ggcacgactg ggccaaggcg ctggtgctgc
      541 cctggcaggc cgatggcctc acccggcccg aactgcagat cgccggcacg ggcgcccacc
      601 acatactggg cctggccgaa gccatgcgcc gcggcctgcc cggcccggtg atcgccgcgc
      661 aggcctgcgc ccatgccctg cccggcaccg atggcgcgcc cgtggccggc tggctgcagg
      721 cggccgccat catcgcccgc cggcccacca cgcaggtgcc caccgcccgg ccgggccctg
      781 cctgccgcat cacttttgcc gccgatcaaa gctggccgcg caccgatgcc gccctcgccc
      841 gcgccgaagt ccgcctgcgc gcgctggccc agccctgcgg cagagacagc ggccctgcct
      901 tcgagatcgg cgtgaagaat gccctgctgg cccgccaggg cacggcgctg aaggcaggcc
      961 gcagcctgcg cccctgccca tccacccgcg ccacaccgga accccacgga tatttgccgc
     1021 caacacccct ttcatccttt ggcgcctaga cttgccgcgc tagaaagcag gaaacgacaa
     1081 gatagcctgc cgggagagac aaccatgccc gagacctctc acgcccccct tccccccgcc
     1141 gatacgctgt ctgccgcgct ggctgccaca ctggccgccc ggctgggcac ggcctttctg
     1201 ggcgaagagg aaacgccaga gcccgaggcg ctggcccagg cggcccattt cctgcgcgag
     1261 gcggccgcca gccgcgcccc cggcctgccg gcgctggcga tcgagccttt cctcaccgcc
     1321 accggccagc gcctcacccg catcgccgtg atcaacgacg acatgccctt ccttgtcgac
     1381 accatcgccg gcgctgtcag caccgagggg ctggcgatcg agcggctggc ccaccccgtg
     1441 ctggcggccg aacgcgatgg cgcgggccgc cttgccgccc tccccgcagg cgacgtgccg
     1501 ggcaccgcgc gcgaaagctt cgtctatctg gaaaccgcgc gggccaatgc ccgcacccgt
     1561 ggccggctgg ccacaggcat ccagcgggcg ctggccgatg gccgcgcggc ggtagccgac
     1621 tgggaagcga tgcgcgcggc gatggcggcc gatgccgcca cccttgcccg gcaggaccgc
     1681 agcgaggcgg ccgccctgct gcgctggctg gcggccgaca tgttcaccct gctcggccat
     1741 gtgcgccaca cgcgcgatgg gggcgccagc gccgcgctgg gcatctgccg ggccagcacg
     1801 cccgccccgc tgccgcccga agcgctggcc gaggcctttg cctggtgcga tgccttcatg
     1861 gccggcggcg gcgggcggct gccgcttatc gtcaaatccg atcatctggc gctggtgcac
     1921 cggcgggtgc cgctcgacct gttcatcgtg cccgagatcg ccggcggccg ggtggaggcg
     1981 ctgtcgatcc acgccggcct gtggaccagc gccgcccttg cctcgccgcc cgcccaggtg
     2041 ccgctgatgc gcgcccagct ggcaacgctg atgggcaagt tcggctttgc cagcgatggc
     2101 catgccggca aggtgctggc ccatgcgctg accagcctgc cgcacgacca gctgatcgcc
     2161 ttttccgacg acgatctcga acgcgtggcg ctggcgatga tgagcctgct cgaccggccg
     2221 cgcccgcgcc tggcgctggt ggcagcgccc atggcccgcc agctgttcgc cttcatctgg
     2281 ctcccgcggg acatgttctc caccgatgtg cgcgaggcga tcacccgcct gctggtggaa
     2341 gccaccggcg cgcgcgtgct ggactggagc ctggaggtgg aaagttcagg ccttgcccag
     2401 ttgcgcttcg tgctggcccg cagcgatcat gcggcccctg gcgcccctgc cgccccggcg
     2461 cccgccggca cagcgcctgc cagggcgccg gccgatgaag ccgcgctgga tgcccagctg
     2521 ggcgccatgc tgcgcggctg ggacgaggcg gtggaggcag agctggccca cagcctgccg
     2581 ccccgccggg cagcggccgt ggcggcgcgc tatgccgggg cctttccgcc ggcctatcgt
     2641 tcgctctatg gcccggcgca ggcggcgata gacattggcc acctgcgcca tctggtggcc
     2701 cgcaagggcc aggcgctgcg ccgggcggtg cgcctctatg ccctggcgag cgacccgccg
     2761 gaacggctgc ggctgaagat ctatcaggcc accggcgcgc tggtgctgtc cgaagtagtg
     2821 cccgcgctgg agaatttcgg cttccgggtg atcgacacca tgcccaccca cctggccatg
     2881 gccagccatg gccaggcgcc ggcgcaaggc gggctgggca ccatccacga tatcctgctg
     2941 gccctgcccg cccctgcccc cgatgccccc gccagccacc cggccgacag cgcccggcgc
     3001 gccgtgctgc accatgcgcc gatcgtggaa gatgcgctgg gcgccatgct gaacggggcc
     3061 ggcgaggatg atcccttcaa ccggctgatc gtcggcgtgg ggctttccgc gcgtgaggcg
     3121 aactggctgc gcgcgctcta ccgctatctc cggcaggcgg gcatgagcca gggcgtggat
     3181 acggcggtgg acgcgctgat gcgcgcgccg gtggtggcgc tggcgctggt ggatctgctg
     3241 cgcgcgcgcc accagcccgg catagacggg cgcgccgccg ccgaggcgct ggccgaggcc
     3301 accttgtcgg ccggccttgg cgaagtggcc gccattcagg acgaccgcct gctgcgcgcc
     3361 tttggcgctg cggtgcgggc gctgctgcgc accaatgcct tcagcccggc cagcacccag
     3421 gcgctggcct tcaagttcga tagcgcgctg gtgccgggcc tgccccgccc gctgccctgg
     3481 cgcgagatct tcgtctatgc ccccgaggtg gaaggcattc acctgcgcgc cggccctgtc
     3541 gcgcgtggcg gcctcagatg gtctgaccgg cgcgatgatt tccgcaccga ggtgttgggg
     3601 ctgatgaagg cccagcgcgt gaagaatgcc gttatcgtgc cgacaggggc caagggcggc
     3661 ttcttcccca agcatctgcc cgatccggcc acccatcgcg ccgcctgggc cgaagggggc
     3721 cgcgccgcct accagacctt cgtgcgcgcg ctgctctcgc tgaccgacaa tctcgtcgat
     3781 ggccagctgg tgcatccggc gggcatcacc atccgcgatg gcgccgatcc ctatttcgtc
     3841 gtcgccgccg acaagggcac cgccaccttc tccgacaccg ccaatgccat cgccgcagaa
     3901 gccggcttct ggctgggcga tgccttcgcc tcgggcggct ctgtcggcta tgaccacaag
     3961 gcgatgggca tcaccgcgcg cggcgcctgg gtctctgtcc agcggcattt ccgcgagatg
     4021 ggcgtggatg tgcaggcaga gccggtgcgc gtggcaggcg tgggcgatat gtcgggcgat
     4081 gtgttcggca atggcatgct gctgtccaag gcgatctgcc tggtcgccgc tttcgatcac
     4141 cgccacatct tcctagaccc cgcccccgat ccggccaccg cctgggccga gcgggcgcgg
     4201 ctgttcgccc tgccgcgctc cagctgggcc gattacgaca gcgcgctgat ctcgaagggc
     4261 ggcggcgtgt ttgcccgcag cctcaaatcc atccccctct cgcccgaggc gcgcgccgcc
     4321 cttggcacag aggccgagac gatcgagccc gatgcgctga tccgcgccat cctcaccgca
     4381 tcggtcgatc tgctgtggtt cggcggcatc ggcacctatg tgaaggcggc cagccaaagc
     4441 gacgccgagg cgggggatcg caccaacgat gccctgcgcg tgaacggcgc gcagctgcgc
     4501 gcgcgcgtgg tgggcgaagg ggcgaacctg ggcgtcaccc aggcggggcg catcgaatat
     4561 gccctggccg gcgggcgcat caacaccgat ttcatcgaca attccgccgg tgtcgattgt
     4621 tccgacaagg aagtgaacat caagatcgcg ctggcctcgg cccggcgcgc cggccgcctg
     4681 gccgaggcgg accgccccgc cctgctggcc ggcatgaccg atgccgtggc cgagctggtg
     4741 ctggacgaca accgccagca ggcgctgggt ctttccgtgg cgcagggcgg cggggtggcg
     4801 gcggtgccgg cctatctcag gctgatcgac cagctggagg aagccggcca gctcgaccgc
     4861 gcgacagaag gcctggccga tgccgaaacc ttcactcgcc gcgccgccgg ccacgatgac
     4921 gggcaggatg gccgcggcct cacccggccg gaactggcgg tgctgctctc cagcgccaag
     4981 ctggccctgc aggcggcgct ggaagccgcc ccgctgctcg atgatccgga actgacgccg
     5041 ctgttcgcgc ccctgctcgc cgccgccttc cccgaggcga tgcggcgcgg ctttgccgcc
     5101 gatatcgccg gccaccagct gcgccgcgag atccttgcca cgggtatcgc caaccgggtg
     5161 gtgaaccggc tgggcctgct cgcccccttc gagctggccg aggaggaagg cgtggggctg
     5221 gccgatgtgg ccgctgcctg ccttgccgca gaagcgctgt ttggcatggc cccgctgtgg
     5281 cgcgcgatcg acacaggggt gatgccagag gccgcccgca ttgccctgat gggcgcggcc
     5341 gccgctggcc tgcgcggcca tatgtcggaa ctgctgcggg cagagccggc cgcccccatg
     5401 gcgctggcca gcgcccttgg ccccaccatc gccgaactgg ccgacgagcg cgaaagccag
     5461 caggaaccgg ccgcccgcgc gcaatcggcc gcacggctgg cggctctcac cgatgccggc
     5521 gcgccggtcg atctggcgat cagcgtcaac cggctggccg atatggcggg cgcggtgggg
     5581 ctggcctatc tggcccatgg ccggcgctgc cacctgccgg cgctggcgca ggccttcgcc
     5641 cggctgggca ccgcgctcga tctcgactgg gcggaagcgg ccgcggcccg cctcgccccg
     5701 gcagacccgt gggagcggct gctggcaggc gggctggcgt gcgatttcca gcagatgcgc
     5761 ctggcccgcc tcgcgcgcct cgccgagggc gatccg
//
LOCUS       DASRYE010000209        10712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_27252,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000209 DASRYE010000000
VERSION     DASRYE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27252"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFF98_10690"
     CDS             <1..251
                     /locus_tag="VFF98_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZU64142.1"
                     /translation="LAGTPGLAALGVLVAAVTAGLKGGAALGGLLMVPLAVPILIFGA
                     GTLASGGTSGAALAGAASLVLVAMAPFAAGAAIRAGRG"
     gene            complement(259..1086)
                     /gene="map"
                     /locus_tag="VFF98_10695"
     CDS             complement(259..1086)
                     /gene="map"
                     /locus_tag="VFF98_10695"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZU64143.1"
                     /translation="MTQYQTVDATDTLLRDGTIKLHGPEGFAGMRKAGLLAAEILDAL
                     VPLVQPGVTTGHIDDIVRQMTLDAGAVPATLGYRGYAHSCCISINHVVCHGIPSDKTF
                     RDGDIVNIDVTPLVDGWHGDTSRMYLVGDVPLKARRLVDVTYECLMIGIEMAKPGARL
                     GDIGHAIQRHAESHRYGVVRDFCGHGVGRLFHDAPEVVHVGHPGTGPELRPGMFFTIE
                     PMINLGKPAVKILEDGWTAVTRDRSLSAQFEHSIGITETGCEIFTASPRGLHKPPYA"
     gene            complement(1176..1961)
                     /locus_tag="VFF98_10700"
     CDS             complement(1176..1961)
                     /locus_tag="VFF98_10700"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HZU64144.1"
                     /translation="MRKIGNRQAGRKLRIWPAVAIGLAALALMAPPAQAASAPAHKVE
                     ANTALYTFSYEYPQKAEAIAALRAWLDSDAARARREIAASAREGAADARQNGYPFNPY
                     DASTSWQVVTDLPGWLSLSGMAADYTGGAHPNHGPIALLWDKAAGQRRQALDLFASKA
                     AFSAALKGPFCAALNRQRAERRGAAIDPHSTDPFDACLDPAEAVVILGGADHQHFTRI
                     GLLLGPYAAGPYAEGDYEVTLPVSPAVLAAVRPPYRAAFAVGR"
     gene            2130..3521
                     /locus_tag="VFF98_10705"
     CDS             2130..3521
                     /locus_tag="VFF98_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HZU64145.1"
                     /translation="MPELVLADRGQAAIAIHLVDKAGLEGWLAKRSAGQRAQAAAQKF
                     RAEPFSHAILAEGEGWLVVAGVADAARLSSWCLGRLAEVLPAGTYRLHPAHAEADIAP
                     ALIGWALGQYRFARYRKDNGTPPEGPRLLLTKAAKAIAPALAEAAAVNLVRDLVNTPA
                     EDMGPADLEHEVERLARASRARLHVTRGEGLEQEFPCVHGVGRAASRHAAPRMIELEW
                     GDPAHPRVAIVGKGVCFDTGGLDIKPSSAMLLMKKDMGGAAHALALARLVIEAGLKIH
                     LHLLIPAVENAVAGNAFRPGDVLRSRAGLSVEIGNTDAEGRLILADALAKAGEEKPVL
                     ILDFATLTGAARVALGPDLPALMARQDATADALVAAGAETGDPVWRLPLYEGYRDYLK
                     SDVADLNNSPASGFAGAIAAGLFLDRFVPEGVDWAHFDTFAWRPAGKPGRPKGGDALG
                     LRAAWALLRQRFG"
     gene            3597..3959
                     /locus_tag="VFF98_10710"
     CDS             3597..3959
                     /locus_tag="VFF98_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZU64146.1"
                     /translation="MAQDHSHNGFLFAAEGGFLSPPGGFTLTGPVPAGSCEGLPVRGD
                     LAHIRLAGQVFVPHYVAPVERRVGSAGAALLQAPGGAVLAQLAAGSPFHMLDTSGGYA
                     WGEAGTLVGYMALGEMEA"
     gene            3963..4895
                     /gene="argC"
                     /locus_tag="VFF98_10715"
     CDS             3963..4895
                     /gene="argC"
                     /locus_tag="VFF98_10715"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZU64147.1"
                     /translation="MAARVFIDGAAGTTGLEIADRLGARAEFELIILDDARRKDSAAR
                     KDALHEADFAILCLHDDAAHEAVAMAEGARVRIIDASTAHRVADGWTYGFPEVVGREA
                     VAGAARVANPGCYPTGFIALMAPLVRAGLLPADFPYTVNAVSGYSGGGKALIERFEED
                     GDIAFRAYGLGMGHKHVPEMTRYAGLAHAPVFAPSVIPAHRGMWVEVPLALSAMAGAA
                     APDALREGLAAFYEGSAIVTVHAAATPDEMLLRRSKAPSDRLDLHVFASQDGSQARLV
                     AVLDNLGKGASGAAVQSLNLMAGLAEATGLALDA"
     gene            5182..5520
                     /locus_tag="VFF98_10720"
     CDS             5182..5520
                     /locus_tag="VFF98_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZU64148.1"
                     /translation="MKKIEAIIKPFKLDEVKEALHEVGVSGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEVVVPDDVAERVVEAIANAAQTGRIGDGKIFIIPVDSALRI
                     RTGERGDDAI"
     gene            5678..7087
                     /gene="glnA"
                     /locus_tag="VFF98_10725"
     CDS             5678..7087
                     /gene="glnA"
                     /locus_tag="VFF98_10725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZU64149.1"
                     /translation="MATAADILKRIKDEEIEWVDLRFTDPKGKWQHLTMVSTVLGEDE
                     LESGLMFDGSSIEGWKAINESDMILKPDLDAVYVDPFSATPMLIVVCDIVEPSTGELY
                     ARDPRSTAKRAEAFVKATGIGDTVYVGPEAEFFLFDDVKFYDGYDGNGFKIDDIELPT
                     NSAKDYEGGNLAHRPRAKGGYFPVAPVDSCVDIRGEMVSTMIGMGLPCDKHHHEVASA
                     QHELGLTFGTLTQTADRMQVYKYVVQQVAQAYGKTATFMPKPIKTDNGSGMHTHISIW
                     DKGNPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSYKRLVPGYEAPVLLA
                     YSARNRSASCRIPYGAGSKAKRVEFRFPDAMANPYLCYAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPADELALVPTVCGSLREALDSLAADYDFLLEGGVFTKDQIEAYLELKWPE
                     VMRWETTPSAVEFDMYYSA"
     gene            7235..7735
                     /locus_tag="VFF98_10730"
     CDS             7235..7735
                     /locus_tag="VFF98_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HZU64150.1"
                     /translation="MSLAIGPAGIALIQAFEQCGRALPGGRFAAYPDPASRAAPWTIG
                     WGSTGPGITRATVWTRAQCDARLVADLARFSARVAGLLAPAPTSQHQFDALVSLAYNL
                     GAERLQASRLLALHRAGDHAGAARAFARWASANGQPMPGLIRRRAAEAALYATPDGQR
                     APPIPQ"
     gene            complement(7729..>10712)
                     /locus_tag="VFF98_10735"
     CDS             complement(7729..>10712)
                     /locus_tag="VFF98_10735"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZU64151.1"
                     /translation="GWRLPVDLAVARLGTGNGALDPQLARGRAHATFTFAKGQLAAPD
                     LALSWPALRLALALKAEPARGAWALAGRASSSGWPVAGVGRISGQAGLALTSARGQGW
                     RLGVDLKGAAAGLKDGSLAALVGDSARLSARLAADSAGRMDLSQAALTGPRLSLALSG
                     QRRADGGFAFAGHGQQVTYGAFSATGSWGRDGPHGEVRLADPLPVLGVKQVVVGITPA
                     PAGAALAITGQSMLGPMDGTLALALPKDGPARLDVRRLAFSGTVAAGSLRLAGGGVDG
                     ALSLSGGGVNGSVELARRDGGQAVDLSLRLTNAHFGGDQPLTIGLAHLEANGLIRRDH
                     TTIAVAGLAQGVGKGRLFIGRLAANAKLEDGSGTINVALGGRRGSRFDLEAQARVAPE
                     KMDLLARGSFGNQPISMPQRARLAAERRPDGGFAGWRLAPAEVDYGGGRVMAEGFLGN
                     GSMDLKLGVVDMPLSLGDVVFADMGLSGRATGLFNWTQQREQMPEANAELMIKGLSRA
                     GLTTTSRPVDVALVGRLEKTALQLRARAAQGGQQNGRLQARVDNLPEQGTLAERLAAG
                     RLFAQLRYGGPADALWRLMALNDLDLSGPVDLAADITGSLRDPLIRGSLAGDDLRLQG
                     PQMGMDITHMATRASFDGSRLVFARLAGKTAGNGEVSGSGMVDFTALGHKGPAIDLRL
                     AAKKAQLVGRADMSLAVTGPLRIVSDGESGTIAGRVVIDMARWRLGQAADVAEIPVIA
                     TQESNRRADIAPASAREVAWSFLVDGAGGNVRLQGLGLDSIWRAAIRLRGPLDDPSLQ
                     GHADLLSGSYEFAGKRFDLTRGLITMDGTTPTDPRLDIAATASVSDINATVTVRGSAL
                     RPEITFSSVPPMPEEELLSRLLFGTSVTSISAPEAVQLGTAVASLHGGGGLDPINRLR
                     GAIGVDRLRIISADPAQDRQTGLAVGKYLGRRVYAEVVSDGRGYSATNLEFRLTSWLA
                     LLGTVSTIGRQSVNAKITHDY"
BASE COUNT         1676 a         3772 c         3618 g         1646 t
ORIGIN      
        1 ttttggccgg caccccgggc cttgccgcgc tgggcgtgct ggtggccgcg gtcaccgccg
       61 ggctgaaggg cggcgccgcg ctgggcgggc tgctgatggt gccgctggcg gtgcccatcc
      121 tcatcttcgg cgccggcacc ctggccagcg gcggcaccag cggcgcggcg ctggcgggcg
      181 ctgccagcct ggtgctggtg gccatggccc cctttgccgc gggcgccgcg atcagggccg
      241 gccggggctg agcttccctt acgcgtaagg cggcttgtgc agccccctgg gcgaggcggt
      301 gaagatctcg cagccggtct cggtgatgcc gatggaatgt tcgaactggg ccgagagcga
      361 gcggtcgcgc gtcaccgccg tccagccgtc ttccaggatc ttcaccgccg gcttgcccag
      421 gttgatcatc ggctcgatgg tgaagaacat gcccggccgc agttccggcc cggtgcccgg
      481 gtggcccaca tgcaccactt cgggcgcatc gtggaacagg cggcccacgc catggccgca
      541 gaaatcgcgc accacgccat agcggtggct ttcggcatgg cgctggatgg catggccgat
      601 atcgcccagc cgggcgccgg gcttggccat ctcaatgccg atcatcaggc attcataggt
      661 cacatccacc agccggcggg ccttcagcgg cacatcgccc accagataca tgcggctggt
      721 atcgccatgc cagccatcca ccagcggcgt cacgtcgatg ttgacgatat cgccatccct
      781 gaacgtcttg tcggacggga tgccatggca cacgacatgg ttgatcgaga tgcagcagct
      841 gtgcgcatag ccgcgatagc ccagcgttgc cggcacggcg ccggcatcca gcgtcatctg
      901 gcgcacgata tcgtcgatat ggccggtggt cacgcccggc tgcaccagcg gcaccagcgc
      961 atcgaggatt tcggccgcca gcagccctgc cttgcgcatg ccggcaaagc cttcgggccc
     1021 gtggagcttg atcgtgccat cgcgaagcaa ggtgtccgtg gcgtcaactg tctgatactg
     1081 ggtcatgagc catacatagg cgcgatggcg acaaaaagcg aggggcgctg catgggcagc
     1141 tccccccggc cctcgccccc gccctacccc ttggcctagc gccccacggc aaaggcggcc
     1201 cgatagggcg ggcgcaccgc cgccagcacg gccggcgaga cgggcagcgt cacttcgtaa
     1261 tccccttcgg catagggccc ggcggcatag ggccccagca gcaggccgat gcgcgtgaaa
     1321 tgctgatgat cggcgccgcc caggatcacc accgcctcgg ccggatcgag gcaggcatcg
     1381 aagggatcgg tgctgtgcgg atcgatggca gcgccgcgcc gctcggcccg ctggcggttg
     1441 agcgcggcgc agaacggccc cttcagcgcg gccgaaaacg ccgccttgct ggcgaacaga
     1501 tcgagcgcct ggcgcctttg gccggcggcc ttgtcccaca aaagggcgat cgggccgtgg
     1561 ttggggtggg cgccgccggt atagtctgcg gccatgcccg aaaggctgag ccagccgggc
     1621 agatcggtca ccacctgcca gctggtggaa gcatcatagg ggttgaaggg atagccgttc
     1681 tgccgggcat cggcggcccc ttcgcgcgcg ctggcggcga tctcgcgccg ggcgcgggcg
     1741 gcatcgctgt ccagccaggc gcgcagggcg gcaatggctt ctgccttctg gggatattcg
     1801 tagctgaagg tgtagagcgc ggtgttggcc tccaccttgt gcgccggggc gctggcggcc
     1861 tgcgccggcg gcgccatcag tgccagggcc gccagcccga ttgccactgc cggccagatc
     1921 cgcagcttgc gaccagcctg ccggttcccg attttccgca tccccatttt cctctcgcgc
     1981 tttggcggcc ggcacggcta gtaagttgct ggccagcccc catatcggcc ggcaccacca
     2041 cagcagaccg gccgttaacc gccgttgacg cagcagatca ggtaagcccc atgaacgcca
     2101 agcccaccac ccccaccgcc gaccatgcca tgcccgaact ggtgctggcc gatcgcgggc
     2161 aggcggccat cgccatccat ctggtggaca aggccgggct ggagggctgg ctggccaaac
     2221 gcagcgccgg ccagcgcgcg caggccgccg cccagaaatt ccgcgccgag cccttcagcc
     2281 acgccatcct tgccgagggc gagggctggc tggtggtggc cggcgttgcc gatgcggcgc
     2341 gcctgtcgag ctggtgcctg ggacggctgg ccgaagtact gccggccggc acctatcgcc
     2401 tgcacccggc ccatgccgag gccgacatcg ccccggcgct gatcggctgg gcgctggggc
     2461 aatatcgctt cgcgcgctat cgcaaggaca atggcacccc gcccgagggg ccccgcctgc
     2521 tgctgaccaa ggccgccaag gcgatcgccc cggcgctggc cgaagcggcg gcggtgaacc
     2581 tcgtgcgcga tctcgtcaac acgccggccg aggatatggg cccggccgat ctggagcatg
     2641 aggtggagcg cctggcccgc gccagccgcg cgcgcctgca cgtgacgcgc ggtgaagggc
     2701 tggagcagga atttccctgc gtgcatggcg tgggccgggc cgcatcgcgc catgccgccc
     2761 cgcgcatgat cgagctggaa tggggcgatc cggcccatcc gcgcgtggcc atcgtgggca
     2821 agggggtgtg tttcgatacc ggcgggctgg atatcaagcc gtcttccgcc atgctgctga
     2881 tgaagaagga catggggggc gccgcccatg cgctggcgct ggcccggctg gtgatcgagg
     2941 cggggctgaa gatccatctg cacctgctga tcccggcggt ggaaaatgcg gtggcgggca
     3001 atgccttccg ccccggcgac gtgctgcgca gccgggcggg cctgtccgtg gagattggca
     3061 ataccgatgc cgaggggcgg ctgatcctgg ccgatgcgct ggccaaggcg ggcgaggaaa
     3121 agccggtgct gatcctcgat ttcgccacgc tgacaggcgc tgcccgcgtg gcgctggggc
     3181 cagacctgcc ggcgctgatg gcccgccagg atgccaccgc cgacgcgctg gtggccgctg
     3241 gcgccgaaac gggcgatccg gtgtggcgcc tgccgcttta cgagggctat cgcgattatc
     3301 tgaagtcgga tgtggccgat ctcaacaaca gcccggccag cggctttgcc ggggcgatcg
     3361 cggccggcct gttcctcgac cgcttcgtgc ccgaaggggt ggactgggcc catttcgaca
     3421 ccttcgcctg gcgcccggcg ggcaagcccg gccggcccaa gggcggcgat gcgctgggcc
     3481 tcagggcggc ctgggcgctg ctgcgccagc ggtttggctg aaccggccgg ccagcgcttg
     3541 cgcaaacccc ggcgcccggc taatcgccgc ccctttcgat gagggacaag cgcaagttgg
     3601 cacaggatca ttcgcataat ggctttctct tcgcggccga gggcggcttt ctcagcccgc
     3661 cgggcggctt tacgctgaca gggccggtgc cggccggcag ctgcgaaggg ttgcccgtgc
     3721 gcggcgatct ggcgcatatc cgcctggccg gccaggtgtt cgtgccgcat tatgtggccc
     3781 cggtggaacg ccgcgtaggt agtgcgggcg ccgccctgct gcaagcgccg ggcggcgccg
     3841 tgctggcgca actggccgcc ggcagcccct ttcacatgct cgacacctcg ggcggttatg
     3901 cctgggggga agccggcacg ctcgtcggct atatggccct tggcgaaatg gaggcttgag
     3961 agatggcagc gcgcgtattc atcgatgggg cggccggcac cacggggctg gaaattgccg
     4021 accggctggg cgcccgcgcc gaattcgagc tgatcatcct ggatgatgcc cgccgcaagg
     4081 acagcgccgc ccgcaaggac gccctgcacg aagccgattt cgccatcctg tgcctgcatg
     4141 acgatgccgc gcacgaggcc gtggccatgg ccgagggcgc ccgggtgcgc atcatcgatg
     4201 ccagcaccgc gcaccgcgtg gccgatggct ggacctatgg ctttcccgag gtggtgggcc
     4261 gcgaagcggt ggcaggggcg gcgcgggtgg ccaatccggg ctgctatccc accggcttca
     4321 tcgcgctgat ggcgccgctg gtgcgcgccg gcctactgcc ggctgatttt ccctacacgg
     4381 tgaatgccgt ctcgggctat tcgggcggcg gcaaggcgct gatcgagcgt ttcgaggagg
     4441 atggcgacat cgcctttcgc gcctatgggc tgggcatggg ccacaaacat gtgccggaaa
     4501 tgacccgcta tgccgggctt gcccatgcgc cggtgtttgc ccccagcgtg atcccggccc
     4561 atcgcggcat gtgggtggaa gtgccgctgg cgctgtcggc gatggccggc gctgccgcgc
     4621 ccgatgccct gcgcgagggg ctggccgcct tttatgaggg cagcgccatt gttaccgtgc
     4681 atgccgccgc cacgcccgac gagatgctgc tgcgtcgttc caaggcgccg agcgaccggc
     4741 tggacctcca tgtgtttgcc agccaggatg gcagccaggc gcggctggtg gcggtgctcg
     4801 acaatctggg caagggggcc agcggcgctg ccgtgcagag cctgaacctg atggccggcc
     4861 ttgccgaagc caccggcctg gcgctggacg catgagccac gggcagagca ttgcccataa
     4921 attcagcact gcctgcccga aagctgggca ggccaggcag gaaaacccgg cagcgcccgc
     4981 aggcaggcag gaaaagccca gcttcattcg cgcaaatccg aggttttcct gcgagatgct
     5041 ttgccctacc aagggcaaac cgactctggc acggatcttg atagattctt accggggtac
     5101 tcggggcatc cggcccgcct ggcgcggcgc cggctctacg cacaggcggg ggtctcaccc
     5161 ctggaaaggg atgttgcagg cgtgaaaaag atcgaagcga tcatcaagcc gttcaagctc
     5221 gatgaggtga aggaggcgct gcatgaagtg ggcgtttccg gcatcaccgt gaccgaggcc
     5281 aagggttttg gtcgccagaa ggggcacacc gagctgtatc gcggagccga atatgtggtg
     5341 gacttcctgc ccaaggtgaa gcttgaggtg gtggtgcccg acgacgtggc cgagcgcgtg
     5401 gtggaagcca tcgccaatgc cgcgcagacc ggccgcatcg gcgacggcaa gatcttcatc
     5461 atcccggttg acagtgccct gcgcatccgc accggcgagc gcggcgacga cgccatctga
     5521 tccatccatt ccaccggccg ggccacaggc atgagggccg cgcccggccg gccagggaac
     5581 tgctttgcat tcaggggtct ggcggcagtt ttctctaatc cactcatacc ccttacccct
     5641 tccccccggg cagggacatt tctggagacc aaaggtaatg gctactgcag ctgacatcct
     5701 caagcgcatc aaggacgagg aaatcgagtg ggtcgatctg cgcttcaccg accccaaggg
     5761 caagtggcag cacctgacga tggtttcgac cgtcctcggc gaggatgagc tggaaagcgg
     5821 cctgatgttc gacggctcgt cgatcgaggg ctggaaggcc atcaacgaat cggacatgat
     5881 cctcaagccc gacctcgatg ccgtctatgt cgatccgttc tcggccaccc cgatgctgat
     5941 cgtggtgtgc gacatcgtcg agccctcgac cggtgagctc tatgcccgcg acccgcgctc
     6001 gaccgccaag cgcgccgaag ccttcgtcaa ggccaccggc attggcgaca ccgtctatgt
     6061 cggcccggaa gccgagttct tcctgttcga cgacgtgaag ttctacgacg gctacgacgg
     6121 caacggcttc aagatcgacg acatcgagct gccgaccaac tcggccaagg actatgaggg
     6181 cggcaacctc gctcaccgtc cgcgcgccaa gggcggctac ttccccgtgg ccccggtcga
     6241 cagctgcgtc gacatccgcg gcgagatggt ttcgaccatg atcggcatgg gcctgccctg
     6301 cgacaagcac caccacgaag tggccagcgc gcagcacgag ctgggcctga ccttcggcac
     6361 gctgacgcag accgccgacc gcatgcaggt ctacaagtat gtcgtgcagc aggttgccca
     6421 ggcctatggc aagaccgcga ccttcatgcc caagccgatc aagaccgaca acggcagcgg
     6481 catgcacacc cacatctcga tctgggacaa gggcaacccg ctgttcgccg gcaacggcta
     6541 tgctggtctc tcggacatgt gcctgtactt catcggcggc gtgatcaagc atgccaaggc
     6601 gctgaacgcc ttcaccaacc ccaccaccaa cagctacaag cgcctggtgc cgggttatga
     6661 ggcccccgtg ctgctggcct attcggcccg caaccgttcg gcctcgtgcc gcattcctta
     6721 cggtgccggt tcgaaggcca agcgcgtgga attccgcttc cccgacgcga tggccaaccc
     6781 ctacctgtgc tatgccgcgc tgctgatggc cggccttgat ggcatcaaga acaagatcca
     6841 tccgggcgaa gccatggaca agaacctcta cgacctgccc gccgacgaac tggccctcgt
     6901 gccgaccgtc tgcggctcac tgcgtgaggc gctggactcg ctggccgccg actacgactt
     6961 cctgctggaa ggcggcgtgt tcaccaagga ccagatcgaa gcctacctcg aactgaagtg
     7021 gcccgaagtg atgcgctggg aaaccacgcc gtcggccgtt gagttcgaca tgtactacag
     7081 cgcctgatct ttcagcgctt tagctatagg gagaagcccg ggtcgcaagg cccgggtttt
     7141 tcttttgggg cgcggcagca ggctggccct gtcctacagg aaaccaattc ggttaaacgc
     7201 gattcgcccg cccaccgcga aaggcgcatc gctcatgtcg cttgccatcg gccctgccgg
     7261 aattgccctg atccaggcgt ttgaacaatg cggccgcgcg ctgcctggcg ggcgctttgc
     7321 cgcctatccc gatcctgcca gcagggccgc gccctggacg atcggttggg gcagcaccgg
     7381 gcccggcatc acccgcgcca cggtctggac gagggcccag tgcgatgccc ggctggtggc
     7441 cgatctggcg cggttttctg ctcgggtggc gggtttgctg gcccctgccc cgaccagcca
     7501 gcaccagttc gacgcgctgg taagcctggc ctataatctg ggggcggagc ggctgcaggc
     7561 ctcgcgcctg ctggcgctgc accgggcagg cgatcatgcc ggggcggcgc gcgcctttgc
     7621 ccgctgggcc agcgccaatg gccagccgat gccggggctg atccgccgcc gggctgccga
     7681 ggcggcgctc tatgccaccc cggatggcca gcgggcgccg cctatccctc agtaatcatg
     7741 cgtgatcttg gcgttgacgc tttgccggcc gatggtggac accgtgccca gcagggccag
     7801 ccagctggtc aggcggaatt ccagattggt ggccgaatag ccccgcccat ccgacaccac
     7861 ctcggcatag acgcgccggc cgagatattt gcccacggcc aagcccgtct gccgatcctg
     7921 cgccggatct gccgagatga tgcgcagccg atcgaccccg atggcgccgc gcagccggtt
     7981 gatcggatcc agcccgccgc cgccatgcag cgaggccacc gccgtgccca gctgcaccgc
     8041 ctctggcgcg gaaatgctgg tgaccgaggt gccgaacagc aggcgcgaaa gcagctcctc
     8101 ctcgggcatc ggcggcaccg aggagaaggt gatttcgggc cgaagcgcac tgcccctgac
     8161 ggtgaccgtg gcgttgatat ccgacacgct ggcggtggcg gcgatatcca gccgcggatc
     8221 ggtgggcgtg gtgccatcca tggtgatgag gccgcgggtg agatcgaagc gcttgccggc
     8281 aaattcatag ctgcccgaca gcagatcggc atggccctgc agcgagggat catccaaggg
     8341 cccgcgcagg cggatcgccg cgcgccagat gctgtccagc cccagcccct gcagccgcac
     8401 attgccgccc gcgccatcga ccaggaagct ccacgccact tcgcgcgcgc tggccggcgc
     8461 gatatcggcg cggcggttgc tttcctgggt ggcgatcacc gggatctcgg cgacatcggc
     8521 cgcctggccc agccgccagc gggccatgtc gatcaccacg cggccggcga tggtgccgct
     8581 ctcgccatcc gagacgatgc gcagcgggcc agtgaccgcc agcgacatat ccgcccggcc
     8641 caccagctgc gcctttttgg ccgccaaccg caggtctatc gccgggcctt tgtggcccag
     8701 cgcggtgaaa tcgaccatgc cgctgccgct cacctcgcca ttgccggcgg ttttgccggc
     8761 cagccgcgcg aacaccagcc gcgaaccatc gaagctggcg cgcgtggcca tatgggtgat
     8821 atccatgccc atctgcgggc cctgcaggcg cagatcatcg cccgccagcg aaccgcggat
     8881 cagcggatcg cgcaggctgc cggtgatatc ggcggcaagg tccaccgggc ccgacagatc
     8941 cagatcgttc agcgccatca gccgccacag cgcatcggcc gggccgccat agcgcagctg
     9001 ggcgaacagg cggccagccg ccagccgctc ggccagcgtg ccctgttccg gcagattgtc
     9061 cacccgcgcc tgcaggcggc cgttctgctg gccgccctgg gcagcgcggg cgcgcagttg
     9121 cagggcggtc ttctccagcc ggcccaccag cgccacatcc accggccgcg aggtcgtcgt
     9181 cagccccgcg cggctgaggc ccttgatcat cagctcggca ttggcctcgg gcatctgctc
     9241 gcgctgctgg gtccagttga acaggccggt ggcgcgcccg ctcagcccca tatcggcaaa
     9301 caccacatcg cccagcgaca gcggcatatc caccacgccc agcttcagat ccatgctgcc
     9361 attgcccagg aagccttccg ccatcacccg gccgccgcca taatccacct cggcaggcgc
     9421 cagccgccag ccggcaaagc cgccatcggg gcgccgctct gccgccaggc gcgcgcgctg
     9481 gggcatgctg atcggctggt tgccgaagct gccgcgggcc agcaggtcca tcttctcggg
     9541 cgccacgcgg gcctgcgctt ccagatcaaa gcggctgccg cgccgcccgc ccagcgccac
     9601 attgatggtg ccgctgccat cctccagctt ggcgttggcg gccagccggc cgatgaacag
     9661 ccgccccttg cccacgccct gcgccagccc ggccacggca atggtggtgt gatcgcggcg
     9721 gatgaggcca ttggcttcca gatgggccag gccgatcgtc agcggctggt cgccgccgaa
     9781 atgggcgttg gtgagccgca gcgagagatc gaccgcctgg ccgccatcgc gccgggccag
     9841 ctccacgctg ccgttcaccc cgccgcccga caggctgagc gcgccatcca ccccgccgcc
     9901 ggccagccgc aaactgcccg ccgccaccgt gcccgaaaag gccagccgcc gcacatccag
     9961 ccgcgccggg ccgtctttcg gcaaggccag cgccagcgtg ccatccatcg gccccagcat
    10021 cgattgcccg gtgatcgcca gcgccgcccc ggccggcgcc ggggtgatgc ccaccaccac
    10081 ctgcttcacc cccagcaccg gcagcggatc ggccagccgc acctcgccat gggggccatc
    10141 gcgcccccag ctgccggtgg ccgaaaaggc gccataggtg acctgctggc catggccggc
    10201 aaaggcaaag ccgccatcgg cccgccgctg gcccgacagc gccaggctga gccggggccc
    10261 ggtgagggcg gcctgcgaga gatccatgcg gccggcgctg tctgccgcca gccgggcgga
    10321 aagccgcgcg ctatcgccca ccagcgcggc cagcgagcca tccttcaggc cggcggcggc
    10381 gcccttgagg tctaccccca gccgccagcc ctgcccgcgc gcagaggtga gcgccagccc
    10441 cgcctgcccg ctgatgcggc cgacgcccgc cactggccag ccgctgctgc tggcccggcc
    10501 ggccagcgcc caggcgccgc gcgccggttc tgccttcagc gccagcgcca ggcgcagcgc
    10561 cggccagctg agcgccagat cgggcgcggc cagctggccc ttggcgaaag tgaaagtggc
    10621 atgggcccgg ccgcgggcca gttgcggatc gagcgcgcca ttgcccgtgc ccagccgcgc
    10681 caccgccaga tcgaccggca ggcgccagcc cg
//
LOCUS       DASRYE010000210         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_153804,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000210 DASRYE010000000
VERSION     DASRYE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153804"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFF98_10740"
     CDS             <1..983
                     /locus_tag="VFF98_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZU64152.1"
                     /translation="AGDLGLPLVPGSAGAVSDYAEARELCDAMGYPVLIKAASGGGGR
                     GMKVVNHADELESQMSQARSEAQAAFGDDTVYIEKYLGNPRHIEFQIFGDGKGEAIHL
                     GERDCSLQRRHQKVLEEAPSPVITAAERARMGGIVAKAMADMAYRGAGTIEFLWENGE
                     FYFIEMNTRLQVEHPVTEAITGLDLVREQIRIADGLPLSVRQEELEFRGHAIECRINA
                     EDPWTFTPSPGLVESYHAAGGLNVRVDSGLYAGYRIPPYYDSMIAKLIVYGRTREGCI
                     MRLKRALAEMAITGPKTSIPLHQALLDQPDFKSGDYTIKWLEQWLAQRNG"
     gene            complement(1118..1585)
                     /locus_tag="VFF98_10745"
     CDS             complement(1118..1585)
                     /locus_tag="VFF98_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU64153.1"
                     /translation="MAHLDQIDRRLLAELQQEGRVTNVELAQRVGLTAPPCLRRVRAL
                     EESGFIRGYHAVLDGARLGFTITVFAMVSLKSQAEDALRAFEDHVRTLPEVRECHMLN
                     GEIDFILKIVSRDLQGFQEFLTSKLTPAPNVASVKTSLTIRTSKNQPGVPMEG"
     gene            1856..>2748
                     /locus_tag="VFF98_10750"
     CDS             1856..>2748
                     /locus_tag="VFF98_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZU64154.1"
                     /translation="MRYDDRLATVLRLTPVGAHVARVQYRQLIDLLGSTPADARGELL
                     DAAYLRLAETAASIAPEVRAAMLAEPALRLRSARLVAALAEGEPRVAEAALNAADLTA
                     EEWADLIPALPPLARALAGTRADLPASARALLARLGLGKRALPAAQAQAQAQAPAAPT
                     PAHPVDEIGAIVRRIERFRRHREAQAPAAPADSPRLPLGEAPTALPELRSFDFASDSE
                     GRIVWCEADVAPMIIGLSLGETPGLYTAIRHHQPLRAIPVELAGAAAIAGTWQVDALP
                     RFDPLGGRFIGYLGRFRRPAAG"
BASE COUNT          441 a          928 c          942 g          437 t
ORIGIN      
        1 ccgccggcga cctgggcctg ccgctggtgc ccggctctgc cggcgcggtg tcggattatg
       61 ccgaggcgcg cgagctgtgc gacgcaatgg gctatccggt gctgatcaag gcggcatcgg
      121 gcggcggcgg gcgcggcatg aaggtggtga accacgccga cgagctggaa agccagatga
      181 gccaggctcg ttccgaggcc caggcggcct tcggcgacga tacggtctat atcgagaaat
      241 atctcggcaa tccgcgccat atcgagttcc agatctttgg cgatggcaag ggcgaggcga
      301 tccatctggg cgagcgcgac tgttcgctgc agcgccgcca ccagaaggtg ctggaagagg
      361 ccccctcgcc ggtgatcacc gccgcagagc gggcccgcat gggcggcatc gttgccaagg
      421 cgatggccga catggcctat cgcggcgccg gcacgatcga gttcctgtgg gaaaacggcg
      481 aattctactt catcgagatg aacacccgcc tgcaggtgga acatccggtg accgaagcca
      541 tcaccggcct cgatctggtg cgcgagcaaa tccgcattgc cgatggcctg ccgctgtccg
      601 tccgccagga agagctggaa tttcggggcc atgccatcga atgccggatc aacgcggaag
      661 acccgtggac cttcaccccc tcgccggggc tggtggaaag ctatcatgcc gccggcgggc
      721 tcaatgtgcg cgtggatagc gggctttatg ccggctatcg catcccgccc tactatgatt
      781 cgatgatcgc caagctgatc gtctatggcc gcacacgtga gggctgcatc atgcggctga
      841 agcgggcgct ggcggaaatg gcgatcaccg ggccgaagac ctcgatcccg ctgcatcagg
      901 ccctgctgga tcagccggat ttcaagagcg gcgattacac catcaagtgg ctggaacagt
      961 ggctggccca gcgcaacggc tgagctgacc tgcaccgggc cacacgaaag gccgccccct
     1021 ttgctgcggg gcggcctttt tgctggcccg ccccttcacc accccagacg cggccattgc
     1081 ggaggcattg cgcagcgcgg cgagcagtgc ggcgggctca gccttccatc ggcacgcctg
     1141 gctggttctt cgaggtgcgg atggtgagcg aggttttcac gctggcgaca ttgggcgccg
     1201 gcgtcagctt gctggtgaga aattcctgga agccttgcag atcgcggctg acgatcttca
     1261 ggatgaagtc gatctcgccg ttcagcatgt ggcattcgcg cacttcgggc agggtgcgca
     1321 catggtcctc gaaggcgcgc agtgcgtcct cggcctggct tttcagcgag accatggcga
     1381 aaacggtgat ggtgaagccc agccgtgcgc catccagcac ggcgtgatag ccgcggataa
     1441 agccggattc ttccagcgcg cgcacgcggc gcaggcaagg cggagcagtc agccccacgc
     1501 gctgggccag ctccacgttg gtcacgcggc cttcctgctg caattccgcc agcaagcgcc
     1561 ggtcaatctg gtccagatgc gccataaccc tcacacgtta cgctggcgca gccggccggg
     1621 tgatcaccgc attgcggcaa accaccaagt catgtccaaa attcttgcgc ccgcccctgg
     1681 gtagctttta tgcgccgaag cgtcccgcga tgcaacatct tgcgggcgag atcttgctgc
     1741 ggctgcctct gggcccgctt cgcccacagc ttgcgccacg caggggctgg cattagggcc
     1801 ggaaacgggc gaaaagaccc gggaatattt acgaaggccc tgctagcaac cgccgatgcg
     1861 ctatgacgac cgcctcgcca ccgttctgcg cctgacacct gtcggggcgc atgtggcgcg
     1921 cgtgcaatat cgccagctga tcgacctgtt gggcagcacc ccggctgatg cgcgcggcga
     1981 actgctggat gccgcctatc tgcgcctggc cgagaccgcc gccagcatcg cccccgaagt
     2041 gcgtgcggcc atgctggcgg aaccggcgct gcggctgcgc tcggcgcggc tggtggcggc
     2101 cttggccgag ggcgagccgc gcgtggccga agctgccctt aacgcggccg atctgaccgc
     2161 ggaggaatgg gccgacctga tccccgccct gccgccgctg gcgcgcgcgc tggcgggcac
     2221 gcgggcggat ctgccggcca gcgcgcgcgc gctgctggcc cggctggggc tgggcaagcg
     2281 ggcgctgccg gcggcacagg cacaggcaca ggcacaggcc ccggcggcgc ccacaccggc
     2341 gcaccctgtc gacgagatcg gcgcgatcgt gcggcggata gagcggtttc gccgccaccg
     2401 cgaggcgcag gccccggccg cgcctgccga ttcgccccgc ctgccgctgg gcgaggcccc
     2461 taccgccctg cccgaactgc gcagcttcga tttcgccagc gacagcgagg ggcgcatcgt
     2521 gtggtgcgag gcggatgtgg cgccgatgat catcggcctc agcctgggcg agacgccggg
     2581 gctctacacc gccatccgcc accaccagcc gctgcgcgcc atcccggtgg aactggcagg
     2641 ggcggccgcc atcgccggca cctggcaagt ggatgcgctg ccccgcttcg atccgctggg
     2701 cgggcgcttc atcggctatc tcgggcgctt ccggcggccg gcagccgg
//
LOCUS       DASRYE010000211         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_206878,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000211 DASRYE010000000
VERSION     DASRYE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206878"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFF98_10755"
     CDS             <1..615
                     /locus_tag="VFF98_10755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZU64155.1"
                     /translation="VLIIPALLPRQAAADAPPPPRKGARPAKARPEPAATADDSAADT
                     PPAPKFGWPLLGAVRRGYAPRGTPNYHDGIDIAAPAGTPVRAAAAGTVRFAGNEPQDY
                     GRLVVIEHKGGWQSAYGFLGRIAVKKGEEVKAGAHIGLVGHSGEAKGDELHFELRQAN
                     KAVDPLAVLSPRARRAPRQAEAAADETPAPKPRRKPRPRAAPQD"
     gene            complement(612..959)
                     /locus_tag="VFF98_10760"
     CDS             complement(612..959)
                     /locus_tag="VFF98_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HZU64156.1"
                     /translation="MGPINWMAVGVAALVAAGLAPVWYGRLLRGEGPPPAALLALLLP
                     AWLMGHNFARVGADVLAAKPWLYWMMSGGFAPAIVWPVLAISGHRSGRFWGHAGYWLL
                     AFLAMGTVFRLMA"
     gene            1046..2443
                     /gene="rimO"
                     /locus_tag="VFF98_10765"
     CDS             1046..2443
                     /gene="rimO"
                     /locus_tag="VFF98_10765"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZU64157.1"
                     /translation="MASEPSSQIIPPAPKVGMVSLGCPKALVDSERILTRLRGDGYQM
                     SADYAGADVVLVNTCGFLDSAKEESLAAIGEAIAENGRVIVTGCMGNEAEAIRARFPA
                     VLAVTGAHQYEDVVQAVHVAAPPSQGPFVDLIPADRDTGEVKLTPRHYSYLKISEGCN
                     HACSFCIIPQLRGKLASRRIDAVLREAEKLVAAGTRELLVISQDTSAYGVDIRHEPRP
                     WKDREVRAHMTDLARELGQLRTADGTPPWVRLHYVYPYPHVDDVIPLMAQGLLTPYLD
                     IPFQHAAPNVLRAMKRPANEAKVLERIRKWREICPDLAIRSSFVVGFPGETEADFQYL
                     LDWLDEAQLDRVGAFRFEPVAGASANDLPGAVPEAVKEERYARLMEKTEAISAARLAA
                     KVGRVIPVIIDEVGEADEEGDIGATGRSQADAPEIDGQVLLRDVPPDLAPGAIVNVLI
                     EDADAHDLFGVIAEG"
     gene            2678..>3100
                     /locus_tag="VFF98_10770"
     CDS             2678..>3100
                     /locus_tag="VFF98_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZU64158.1"
                     /translation="MAESSSPPPRIFTAALVVIGDEILSGRTQDKNIAQVASWLGVQG
                     IRLREVRVVADDAAAIAEAVNTLRARNDYLFTTGGIGPTHDDITVDAIATAVGVPVIV
                     HPEARAILEDYYTNRGGLTEARLRMARAPEGASLIPNRY"
BASE COUNT          533 a         1117 c          994 g          456 t
ORIGIN      
        1 gtgttgatca tcccggcgct gctgcccagg caagccgcgg cggatgcgcc cccgccgccg
       61 cgcaaaggcg cccgccccgc caaggcccgc ccggaaccgg ccgccaccgc cgacgacagc
      121 gccgcagaca cgccccctgc ccccaaattc ggctggccgc tgctgggcgc ggtgcggcgc
      181 ggctatgccc cgcgcggcac gccaaactat catgacggga tcgacatcgc cgccccggcc
      241 ggcactccgg tgcgcgccgc tgctgccggc accgtgcgct ttgccggcaa cgagccgcag
      301 gattacggca ggctggtcgt catcgagcac aagggcggct ggcagagcgc ctatggcttc
      361 ctgggccgca tcgcggtgaa aaagggcgag gaagtgaagg ccggcgcgca tattggcctt
      421 gtgggccaca gcggcgaggc caagggcgac gagctgcatt tcgaactgcg ccaggccaac
      481 aaggcggtgg acccgctggc cgtgctgagc ccgcgcgcgc gccgcgcgcc gcgccaggcg
      541 gaagcggccg cagacgaaac ccccgcgccc aagccgcgcc gcaagccccg cccgcgcgcc
      601 gcgccgcagg attaggccat caggcggaac accgtgccca tggccagaaa ggccagcagc
      661 caatagcccg catggcccca gaagcgcccg ctcctgtggc ccgagatcgc cagcaccggc
      721 cacacgatcg ccggcgcgaa cccgcccgac atcatccagt agagccaggg cttggcggcc
      781 agcacatcgg cccccacgcg ggcgaaattg tggcccatca gccaggccgg cagcagcagc
      841 gccagcaagg cggccggcgg cggcccctca ccgcgcagca gccggccata ccacaccggc
      901 gccaggcccg ctgccaccag cgctgccacg cccaccgcca tccagttgat cgggcccatt
      961 gcctttgcgt cctttatctt gcgcgtgtgc cgccggaatg cccgctgtag cgaaggaaag
     1021 gctttgcgtg taggggcttc tcgctatggc aagcgaaccc tcctcccaga tcatcccccc
     1081 cgcccccaag gttggcatgg tcagcctcgg ctgccccaag gcgctggttg attccgaaag
     1141 gattcttacc cgcctgcgcg gcgatggcta tcagatgagc gccgattacg ccggcgccga
     1201 cgtggtgttg gtgaacacct gcggcttcct cgattcggcc aaggaggaaa gcctggccgc
     1261 catcggcgag gcaatcgccg agaacgggcg ggtgatcgtc accggctgca tgggcaatga
     1321 ggcagaggcc atccgcgccc gcttccccgc cgtgctggcc gtgaccggcg cccaccagta
     1381 tgaggatgtg gtgcaggcgg tgcatgtggc agcccccccc tcacaggggc ccttcgtcga
     1441 cctgatcccg gccgaccgcg acacgggcga ggtgaagctg accccgcgcc actattccta
     1501 tctgaagatc tcggaaggct gcaaccatgc ctgcagcttc tgcatcatcc cccaactgcg
     1561 cggcaagctg gccagccgcc gcatcgatgc ggtgctgcgc gaggcggaaa agctggtggc
     1621 cgccggcacc cgcgaactgc tggtgatcag ccaggatacc tcggcctatg gggtggatat
     1681 ccgccacgag ccgcgcccct ggaaagaccg cgaggtgcgc gcccacatga ccgatctggc
     1741 ccgcgaactg ggccagctgc gcacggcaga cggcaccccg ccctgggtgc gcctgcatta
     1801 cgtctatccc tatccgcatg tggatgatgt gatcccgctg atggcacagg ggctgctgac
     1861 gccctatctc gacatcccct tccagcatgc cgcccccaat gtgctgcgcg ccatgaagcg
     1921 cccggccaat gaagccaagg tgctggaacg catcaggaaa tggcgcgaga tctgccccga
     1981 tctggcgatc cgctccagct tcgtcgtcgg cttcccgggc gagacggaag ccgatttcca
     2041 atatctgctc gactggctgg acgaggccca gctcgaccgc gtgggcgcct tccgcttcga
     2101 gccggtggcg ggcgcttcgg ccaatgatct gcccggcgcc gtgcccgagg cagtgaagga
     2161 agagcgctat gcccggctga tggaaaagac cgaggcgatt tcggcggcca ggcttgccgc
     2221 caaggtgggc cgggtgattc ccgtcatcat cgacgaggtg ggcgaggccg acgaggaggg
     2281 cgatatcggc gccaccggcc gcagccaggc cgatgcgccg gaaatcgacg ggcaggtgct
     2341 gctgcgcgac gtgccgccgg atctggcccc cggcgcaatc gttaatgtgt tgatagaaga
     2401 cgcagacgcc cacgacctgt tcggggtgat cgccgaaggc taaccaaagc gaaccatggg
     2461 ggtttaaccc gattgccttt tacgcaacag gtagaacccc tttcgcattt gcagcaacaa
     2521 ccgttttcag gcttaaggag cgggcctttc aggcatcctc tcccaagact tccacgggcc
     2581 ggtcgcaaga tcggcccttt ttttgcgcgc cttgcacgca gagctggccc cttcaccaaa
     2641 ctggccacac cggccacttt gtcctaaccc tttgcccatg gccgaatcgt cttccccccc
     2701 gccccgcatc ttcactgccg ccctcgtcgt catcggcgac gagatcctct cgggccgcac
     2761 gcaggacaag aacatcgccc aggtggccag ctggctgggc gtgcagggca tccgcctgag
     2821 ggaagtgcgc gtggtggccg atgatgccgc cgccattgcc gaggcggtga acacccttcg
     2881 cgcgcgcaac gactatctgt tcaccaccgg cggcatcggc cccacgcatg acgacatcac
     2941 cgtcgatgcg atcgccacgg cggtgggcgt gccggtgatc gtgcaccccg aggcgcgggc
     3001 gattctggag gactattaca ccaaccgcgg cggcctcacc gaggcgcggc tgcgcatggc
     3061 ccgcgcgcca gagggcgcca gccttatccc caaccgctat
//
LOCUS       DASRYE010000212         7579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_257059,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000212 DASRYE010000000
VERSION     DASRYE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257059"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VFF98_10775"
     CDS             complement(<1..2201)
                     /locus_tag="VFF98_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZU64159.1"
                     /translation="MTSGLLRTTCAYCGVGCGISARVEGPRALAIRGDEEHPANHGRL
                     CSKGTHLAETVGLEDRLLQPLIGEKTASWSEALGEVARRLRATLDEHGPEAVAFYVSG
                     QLLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAAHNRAFGEDVVPCSYTDLDAAD
                     LILLVGSNTAWCHPVVWQRIEAARAARGTKIVVIDPRRTETAEQADLHIPLAPDGDVA
                     LFNALLAALHACGLTSEAGLAAPEGFWAALPCDPGVDAALFARLVEMVAAHPRMVTLF
                     SQGANQSTSGTDKGNAIINLHLATGRLNAPGMGPFSITGQPNAMGGREVGGLANMLAC
                     HLGFSAPELADTAAFWNAPHICAGPGLKAVDMFRAVHEGRIKFLWVMATNPAVSMPDA
                     SFVRAALERCPTLVVSEVIADTDTARHAHIRLPAHGWGEKDGTVTNSERRISRQRALL
                     APPGEARADWWIIAEVAAQLGWADSFAYRGPADIWREYASMTALATKHGKALDLTAKA
                     ALSDAAYDALAPFQWGGAHPLAGGYPTPDGRARLVAVRPPAPAEADPAFPLRLNTGRY
                     RDQWHTMTRTGLSPTLSQHRREGLAEVHPADAARLGLAEGGLARIATAQGAAVFRVSL
                     SAGQRPGEIFVPMHWTDEMAGGTGGGARANRLPGQATDPVSGQPGFKNTPASAAPVRP
                     AWRGFLVSRTPIMPPPLLYWSRARVAGGWLYELAGETDSLDPASLLPAGDRL"
     gene            complement(2198..2566)
                     /gene="nirD"
                     /locus_tag="VFF98_10780"
     CDS             complement(2198..2566)
                     /gene="nirD"
                     /locus_tag="VFF98_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HZU64160.1"
                     /translation="MSPQPLSGEWIDIGPVGQISPGNARTLPVQGGEEIAIFHTLNNE
                     FYALVNKCPHKHGPLSQGIVHGTVVTCPLHSWNISLKTGEAQGEDKGCVPTIPLKVDA
                     GRLYLQRAALMAARAPERAA"
     gene            complement(2563..5076)
                     /gene="nirB"
                     /locus_tag="VFF98_10785"
     CDS             complement(2563..5076)
                     /gene="nirB"
                     /locus_tag="VFF98_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HZU64161.1"
                     /translation="MTAPDTQISAPAACGQRERLVVIGNGMAGCRAVEEILARDAARF
                     AITIFGAEPRVNYNRIMLSPVLAGEKSFDEIVINTAQWYADNGIALVSGDPVVAVDRA
                     AQTVLSASGRIEPYDRLLIATGSEPFIIPVPGKDLAGVVSFRDLDDVDRMLAAADQGG
                     HAVVIGGGLLGLEAAHGLTLRGMQVTVLHLMPTLMERQLDEAAGWLLKSELERRGQTI
                     LTGADTAEILGRDGHVAGVKLKDGREIPADIVVMAVGIRPNTALARSAGLDVERGILV
                     DDHMLTSDPAVMAVGECVQHRGICYGLVAPLWEMCRALADQATGQPTGYTGSVTSTKL
                     KVSGIDLFSAGDFSGGDGAEDIVMRDAARGLYKRVVVKEGRLVGAVLYGDTADGSWYF
                     DLLKKAEDISDIREALIFGQAFAQGGGALADPNAAVAALADDAEICGCNGVSKGTVVA
                     CITGGAHSLDAVRGQCKASASCGSCTGLVESLLKVTLGAEVEAGPKTLCKCTSFTHDD
                     VRRLIPERGLKAIPQVMQELGWKTPDGCASCRPALNYYLLCAWPGEYLDDQQSRFVNE
                     RLHANIQKDGTYSVVPRMWGGVTTPAELRAIADAADKYNARLVKVTGGQRIDLFGIRK
                     EDLPAIWGDLNAAGMVSGHAYGKSLRTVKTCVGSEWCRFGTQDSTGLGIKTEQMTWGS
                     WMPHKFKIAVSGCPRNCAEATIKDFGVICVDSGYELHVGGNGGIHVRATDLLCKVATE
                     RQALDVCAAFIQLYREQAHYLERTAPWIERVGLDHVKAALFDTADAVAVLAARFRYSQ
                     QFMQDDPWAKRAAGAEAELHQHLAEFRPFPAIAYKEAAE"
     gene            complement(5307..6515)
                     /locus_tag="VFF98_10790"
     CDS             complement(5307..6515)
                     /locus_tag="VFF98_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64162.1"
                     /translation="MTQELAIGFLPLVDACLPILAHEHGFAEAEGLSLRLVRDVSWAT
                     VLDRLLYGQTDAAHLVAPLAIATTLGRGRPAKPLSVPFVLGLNGNAITLRSDLAAYVC
                     PAGGLGDPAAVGAALAQVAHRRMASGRALRLGVVHRYSSHNYMLRYWLAACGLRPDQD
                     VEIATIAPPFCADALEAGEVDGICVGEPWNSVAVERGVGTIVLATAQIWRRGVEKVLA
                     WRESVMEERRPQAEALVRALVAAARHFIDPANWDANAALLARRAYLDASPEIIRRAIS
                     DRLLLRQGGEPVHYPDFMFQYREAANFPWVSQAQWLYTQMLRWEGGAFDSVEAARAGR
                     VFRPDVYRAALIGSGEPLPGASSKVEGSLAGPMAVGTQQGLITMEPNHFFDGRIFDPA
                     AMPAYLAGLP"
     gene            complement(6512..7150)
                     /locus_tag="VFF98_10795"
     CDS             complement(6512..7150)
                     /locus_tag="VFF98_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZU64163.1"
                     /translation="MRIAVVDESAARASVIREGLAEIEDCEIFVVTERRGLVAHIGQI
                     APDIVLIDLGNPSRDVLEEYFAVSRALARPIAMFVDESDEEALAASVEAGVSSYVVDG
                     LAPHRIRPILDLAVRRFNAFSRLEADLADARGRLAEREAVDKAKRILMQSRGLAEHEA
                     YAELRATAMRQSRRIVDIAEAILTADQLLNGPPPGPSARRPTGRKGHDRTAP"
BASE COUNT         1178 a         2575 c         2641 g         1185 t
ORIGIN      
        1 agccggtcgc ccgccggcag caggctggcc ggatccaggc tgtcggtttc gccggccagt
       61 tcatagagcc agccgccggc cacccgcgcc cggctccagt agagcagcgg cggcggcatg
      121 atcggcgtgc ggctgacgag aaagccccgc caggccggcc gcacgggcgc tgcgctggcg
      181 ggcgtgttct tgaagccggg ctggcccgag acgggatcgg tggcctggcc gggcagccgg
      241 ttggcgcggg cgccgccgcc ggtgccgccg gccatctcgt cggtccagtg catcggcacg
      301 aagatctcgc ccgggcgctg gccggcgctg aggctgacgc ggaacaccgc ggccccttgc
      361 gcggtggcaa tgcgcgccag cccgccctcg gccagaccca gcctggcggc atcggccggg
      421 tgcacttcgg ctagcccctc gcgccggtgc tgcgagagag tgggcgagag gcccgtgcgg
      481 gtcatcgtgt gccactggtc gcgatagcgc ccggtgttca gccgcagcgg aaaggcaggg
      541 tctgcctcgg ccggggcggg ggggcgcacg gccaccagcc gggcgcggcc atcgggcgtg
      601 ggatagccgc cggccagcgg atgcgccccg ccccactgga agggcgccag cgcgtcatag
      661 gcggcatcgc tgagcgcggc cttggcggtg aggtccagcg ccttgccgtg tttggtcgcc
      721 agcgccgtca tgctggcata ttcgcgccag atatcggccg ggccgcgata ggcgaagcta
      781 tccgcccagc ccagctgcgc ggccacttcg gcgatgatcc accagtcggc ccgggcctcg
      841 cctgggggtg ccagcagggc gcgctggcgg ctgatgcgcc gctccgaatt ggtgacggtg
      901 ccatccttct cgccccagcc atgagcgggc aggcggatgt gggcgtggcg ggcggtgtcg
      961 gtgtcggcga tcacttcgga gacgaccagc gtggggcagc gttccagcgc ggcgcgcacg
     1021 aagctggcat cgggcatcga aacagccggg ttggtggcca tcacccagag gaacttgatg
     1081 cgcccttcat gcacggcgcg gaacatgtcc accgccttca gccccggccc ggcgcagatg
     1141 tggggggcat tccagaaagc ggcggtgtcg gccagttccg gcgccgagaa gcccaggtgg
     1201 caggccagca tgttggccag cccccccact tcgcgcccgc ccatcgcatt gggctggccg
     1261 gtgatggaaa agggccccat gcccggcgcg ttgaggcggc cggtggccag gtgcaggttg
     1321 atgatggcat tgcccttgtc cgtgcccgag gtggactggt tggccccctg gctgaacagg
     1381 gtgaccatgc gcggatgggc ggccaccatc tccaccagcc gggcaaacag cgcggcatcc
     1441 acaccgggat cgcagggcag cgccgcccag aagccttcgg gcgcggcaag gccggcctct
     1501 gatgtcaggc cacaggcatg caaggcggcc agcagggcgt tgaacagcgc gacatcgcca
     1561 tcgggggcca gcgggatgtg gaggtctgcc tgctcggcgg tttcggtgcg gcgcggatcg
     1621 atcaccacga tcttggtgcc gcgcgcggcg cgcgccgcct ctatccgctg ccacaccaca
     1681 gggtggcacc aggcggtgtt gctgcccacc agcaggatga ggtcggccgc gtccagatcg
     1741 gtgtaggagc agggcaccac atcctcgcca aaggcgcggt tgtgggcggc gaccgcgctg
     1801 gccatgcaca ggcgcgaatt ggtgtcgata ttggcgctgc cgatgaagcc cttcatcagc
     1861 ttgttggcca cgtaataatc ttcggtcagc agctggcccg aaacatagaa ggccaccgct
     1921 tcgggcccgt gttcgtccag cgtggccctg aggcgccggg ccacttcgcc cagcgcctcg
     1981 ctccagcttg cggtcttctc gccgataagc ggctggagca gccggtcttc cagacccacc
     2041 gtctcggcca gatgcgtgcc cttggagcac aggcggccgt ggttggcggg gtgttcttca
     2101 tcgccgcgga tggcaagggc gcgcggccct tccacccggg cactgatgcc gcagcccaca
     2161 ccgcaatagg cgcaggtggt gcgcagcagg ccgcttgtca cgcggcccgt tccggcgcgc
     2221 gcgcggccat aagtgcggcg cgctgcagat agaggcgccc ggcatccacc ttcagcggga
     2281 tggtgggcac gcagcccttg tcctcgccct gggcctcgcc ggttttcagc gagatgttcc
     2341 agctgtgcag cgggcaggtg accaccgtgc catgcacgat gccctggctg agcggcccgt
     2401 gcttgtgcgg gcacttgttg accagcgcgt agaactcgtt gttcagcgtg tggaagatgg
     2461 cgatctcctc gccgccctgc accggcaggg tgcgggcgtt gccggggctg atctggccca
     2521 cagggccaat gtcgatccac tcgccgctca ggggttgggg gctcattcgg cggcttcctt
     2581 gtaggcgatg gcggggaagg ggcggaattc ggccaggtgc tggtgcagct cggcctcggc
     2641 gccggcggcg cgtttggccc aggggtcgtc ctgcatgaac tgctgcgaat agcggaagcg
     2701 cgcggccagc acggccaccg catcggccgt gtcgaacagg gcggccttca catggtcgag
     2761 gcccacccgc tcgatccagg gggcggtgcg ttcgagataa tgggcctgct cgcggtagag
     2821 ctggatgaag gcggcgcaga catcgagggc ttgcctctcg gtggccacct tgcacagcag
     2881 atcggtggcg cgcacatgga tgccgccatt gccgcccaca tgcagctcgt agcctgaatc
     2941 gacgcagatc accccgaagt ccttgatcgt cgcctcggcg cagttgcgcg ggcagcccga
     3001 tacggcgatc ttgaacttgt gcggcatcca gctgccccag gtcatctgct cggtcttgat
     3061 gccaaggcct gtggaatcct gcgtgccaaa gcggcaccat tccgatccca cgcaggtttt
     3121 caccgtgcgc aggcttttgc cataggcatg gcccgagacc atgccggcgg cattgagatc
     3181 gccccagatc gccggcaggt cttccttccg gatgccgaac agatcgatcc gctggccgcc
     3241 ggtcaccttc accaggcggg cgttatactt gtcggcggca tcggcaatgg cgcgcagctc
     3301 ggcaggggtt gtcaccccgc cccacatgcg cggcaccacc gaataggtgc catccttctg
     3361 gatattggca tgcagccgct cgttgacgaa acggctctgc tggtcgtcca gatattcgcc
     3421 cggccaggcg cacagcagat aatagttcag cgccggccgg cacgaggcgc agccatcggg
     3481 ggttttccag cccagctcct gcatcacttg cggaatggcc ttcaggccgc gttccgggat
     3541 caggcggcgc acgtcgtcgt gggtgaagct ggtgcatttg cacagcgtct tggggccggc
     3601 ttccacctcg gcgcccagcg tgaccttcag caggctttcc accagcccgg tgcagctgcc
     3661 gcagctggcc gaggccttgc actggccgcg caccgcatcg aggctgtgcg ccccgccggt
     3721 gatgcaggcc accaccgtgc ccttggaaac gccgttgcag ccgcagatct cggcatcgtc
     3781 tgccagggct gcaacggcgg cattagggtc cgccagggcg cctccgccct gggcaaaggc
     3841 ctggccgaag atcagcgcct cgcggatgtc ggagatgtcc tcggccttct tcagcaggtc
     3901 gaaataccag ctgccatcgg cggtgtcgcc atagagcacg gcgcccacca gccggccttc
     3961 cttcaccacc acgcgcttgt agaggccgcg ggcggcatcg cgcatgacga tatcctcggc
     4021 cccgtcgccg ccggaaaaat cgccggccga gaacaggtcg atgcccgaga ccttgagctt
     4081 ggtggaagtg acagagccgg tgtagccggt gggctggcca gtggcctgat cggccagcgc
     4141 ccggcacatt tcccacagcg gcgccaccag gccatagcag atgccgcggt gctgcacgca
     4201 ttcgcccacg gccatcaccg ccgggtcgct cgtcagcatg tggtcgtcca ccagaatgcc
     4261 gcgttccaca tcaaggccgg cgctgcgggc aagcgcggtg ttggggcgga tgcccaccgc
     4321 catcaccacg atatcggcgg ggatctcgcg cccgtccttc agcttcacgc cggccacatg
     4381 gccatcgcgg ccgaggatct cggcggtgtc ggcgccggtg aggatggtct ggccgcgccg
     4441 ttccagctcg cttttcagca gccagccggc ggcctcgtcc agctggcgct ccatcagcgt
     4501 gggcatcagg tgcagcacgg tcacctgcat gccgcgcaag gtcaggccgt gggccgcctc
     4561 cagccccagc aggccgccgc cgatgaccac cgcatggccg ccctgatcgg ccgccgccag
     4621 catcctgtcg acatcgtcga gatcgcggaa ggagacgacg ccggccagat ccttgcccgg
     4681 caccgggatg atgaagggct cggaaccggt ggcgatcagc aggcggtcgt aaggctcgat
     4741 gcgacccgaa gcgctcaaga cggtttgcgc ggcgcgatcg accgccacta ccggatcgcc
     4801 cgacaccagc gcgatgccat tatcggcata ccattgcgcg gtgttgatga cgatctcgtc
     4861 gaagcttttc tcgccggcca gcaccggtga gagcatgatg cggttgtagt tcaccctggg
     4921 ctcggcgccg aagatggtga tggcaaagcg cgcggcatcg cgggcgagga tctcctccac
     4981 cgcgcggcag ccggccatgc cattgccgat caccaccagc ctttcgcgct ggccacaggc
     5041 ggcgggggca ctgatttgcg tgtctggcgc tgtcaccagc attctccatt gccaggcatg
     5101 gcaaacgcaa aaaagccgcc tcgagccaag cccgtcaggg cctgaaccgg gcggctgcgt
     5161 tgccacacgt tgttgcgaaa aaagaggcgg gtgaaaaagg ctccgtcatt ggagcgcccg
     5221 gccatctcag tcttcaatct atccgcgcgc ggcggcgatg acaagcggat tttgcgccgc
     5281 agcaagtgcc cggcgcccga tgggcatcag ggcaggccag ccagataggc cggcatggcg
     5341 gcgggatcga aaatccgccc gtcgaagaaa tggttgggtt ccatggtgat aagcccctgc
     5401 tgggtgccca cggccatggg cccggcaagg ctgccttcca ccttggaact ggcgccgggc
     5461 aggggctcgc cgctgccgat gagtgcggcg cggtagacat cggggcggaa cacccggccg
     5521 gccctggcgg cctccaccga gtcgaaagcg ccgccttccc agcgcagcat ctgcgtgtag
     5581 agccactgcg cctggctgac ccaggggaag ttggccgcct cgcgatactg gaacatgaag
     5641 tcggggtagt gcaccggctc gccgccctgg cgcagcagca gccggtcgga tatcgcgcgg
     5701 cggatgatct cggggctggc atcgagatag gcgcgccggg ccagcagggc ggcattggca
     5761 tcccagttgg ccggatcgat gaaatgacgg gcggcggcca ccagcgcgcg caccagcgct
     5821 tctgcctgcg ggcggcgctc ttccatcacg ctttcgcgcc aggccagcac cttttccacg
     5881 ccgcgccgcc agatctgggc agtggccagc acgatggtgc ccacgccgcg ttccaccgcc
     5941 acgctgttcc acggctcgcc cacgcagatg ccgtccacct cgcccgcctc cagcgcatcg
     6001 gcgcagaaag gcggggcgat ggtggcgatc tccacatcct gatcgggcct caggccacag
     6061 gccgccagcc aatagcgcag catgtaattg tggctggaat agcggtgcac cacccccagc
     6121 cgcagcgccc gcccgctggc catgcgccga tgcgccacct gcgccagcgc cgcgcccacg
     6181 gcggccggat cgcccagccc gcctgccggg cacacatagg cggcgagatc gctgcgcagg
     6241 gtgatggcat tgccgttcag ccccagcacg aaaggcaccg acagcggctt tgccggccgc
     6301 ccgcgcccca gcgtggtggc gatcgccagc ggggcgacca gatgggcggc atcggtctgg
     6361 ccatagagca gccggtcgag cacggtggcc cagctgacat cgcgcaccag ccgcaaggac
     6421 aggccctcgg cctcggcaaa gccatgttca tgggccagga tcggcaggca ggcatccacc
     6481 aggggcagaa aaccgatggc aagttcttgg gtcatggcgc ggtcctgtcg tgccccttcc
     6541 ggcctgtggg gcgccgggca gatggccccg ggggcgggcc gttcagcagc tggtcggcgg
     6601 tcaggatcgc ctcggcaata tcgacgatcc gccggctctg gcgcatggcg gtggcgcgca
     6661 gttcggcata ggcttcgtgt tcggcaaggc cccggctctg catcaggatg cgcttggcct
     6721 tgtccaccgc ctcgcgctcg gccaggcggc cgcgggcatc ggcaaggtct gcctccagcc
     6781 gcgagaaggc gttgaagcgg cgcacggcca gatcgaggat cgggcggatg cggtgcggcg
     6841 ccagcccgtc caccacatag gacgacaccc ccgcctccac cgaggcggcc agcgcttcct
     6901 cgtcgctttc atcgacgaac atggcgatcg gccgggccag cgcgcggctg acggcgaaat
     6961 attcctccag cacgtcgcgg ctggggttgc cgaggtcgat cagcacgatg tccggggcga
     7021 tctggccgat atgcgccacc agcccacgcc tttcggtgac cacgaagatc tcgcagtcct
     7081 ctatctcggc cagcccttcg cggatgaccg aggcgcgtgc ggcgctttcg tcgacaacgg
     7141 caatgcgcat caaccccgca tgccagagca caggccgcct gcgcaagccg gaaggaagga
     7201 gaatgccccg gccatgcttt tcaaggcttt cccgcaggtg ttcgttcaca tgttgttcag
     7261 cagggcaagt caaacgcggc tgacctgccc gcgcatgcgg atggcggggg gctggcgcct
     7321 gtcatcaaag cgcaatgctg cgatggaaag gcccgggcct gctgccgcct gcatgcgcgg
     7381 gggcaggccg gggcatcatt tgcgatacta gggatcagga cgggcacatg tcggcacggc
     7441 aggatgaaaa tggcgtgggc acggatggcg ctggcgagac caccggcttt gccctgccgc
     7501 gcggggtgca gctgcgctat ctggggctgg cgggggccgg cctgttggcg ctggcggggg
     7561 cctcggcgct gggccactg
//
LOCUS       DASRYE010000213        10430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_153947,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000213 DASRYE010000000
VERSION     DASRYE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153947"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFF98_10800"
     CDS             complement(<1..582)
                     /locus_tag="VFF98_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZU64164.1"
                     /translation="MTLLSADSPEAATPMIENRTYEEISVGDSASLTRTLTHQDIELF
                     ALVSGDVNPAHMDPAYAATDMFRKVIAHGLWGAGLISAVLGTHLPGPGTIYLGQTLKF
                     LRPVGLGDTITASVRVADKREKGRLTLACSCANQQGETVIEGEADVIAPQEKLRRPAV
                     SLPEIRLAEHDAYRRLVHAAAGRAVPTAVAHPCS"
     gene            736..1260
                     /locus_tag="VFF98_10805"
     CDS             736..1260
                     /locus_tag="VFF98_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64165.1"
                     /translation="MPAARLAAALGLAMASQAPAALAQSCHPLAGHWRYDAAASHVGS
                     GLSFNPYYAVSGIDLVLEQGAGGLTQSWHLTGPHLDETDRYTTPADGTLAPTGTRSAL
                     NAVPQAVSGQWESCTLVEEGRASLFGQTVWTRSRFFVAADNRRLTITQSSHSDLGDVE
                     RTLIFTRTDERPLP"
     gene            1257..2777
                     /locus_tag="VFF98_10810"
     CDS             1257..2777
                     /locus_tag="VFF98_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64166.1"
                     /translation="MSPLPLTRRALIGAAALAPLATQAAAATRPATAITLDPHAPTTP
                     FPHYWEACVGSDRAAVAMRAQWQQDLALARHEAGFRSVRFHGLFDDEMGLCRSIGPNG
                     PELNFLYIDQIFDAMLAIGVKPYVELSFMPLPLASDGNTIFWYKGNTSPPKDMALWVQ
                     VIEGLARHLLARYGAAEVTGWRFEVWNEPNLNFWAGTQGQYFQLYAATARALKRVNPA
                     LQVGGPATAQLMWIDDFLRFCAADNAPVDFVSSHIYADDPQENLFGQDLGLPREQVIP
                     RALQVANGKIRASAFPRIPLVISEWTSQNPAFIAQTIRDCAGLCDTFSYWTFDNIFEE
                     HAPIPGFGNTLYGLIGQSGVARPSFRAFQLLHRLGTQRIDTGSAPLIATRREDGTLAI
                     LAWNLITNADVSGQTGNPMNAPGEEPILHGPPRDLLLRLGGGARRRARVTGVDLAADP
                     VTLAYAAMGRPASPTAAQITALRKAGALPQARPLAGGPGGFHLALPANALALIEIG"
     gene            2955..4700
                     /locus_tag="VFF98_10815"
     CDS             2955..4700
                     /locus_tag="VFF98_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZU64167.1"
                     /translation="MSPEKNPARALPAFSRMLRLTRPNREDAIAFVILAGLALVVFRG
                     AQGAVEPLSRLHLEPLLLDPGQLPYYALRTILRMFAALFASTVFTLVVATLAARSRHL
                     GQIIVPALDILQSVPILGFLTFTVTFFMGLFPGNVLGVELASVFAIFTSQAWNMAFSF
                     YQSLRSLPSDLEEVCQSFALSPVRRFLKLDLPFATPALVWNAMMSMSGGWFFVVASEA
                     ITVGNTTVTLPGIGSWLALAIEKQDFTAVAEAVGTMAVVIVAYDQLFFRPIVAWADRF
                     RFEQTASTERPESWVYDMLRRTRLLPLLFAPVSAIGRLLLALEPARRARRLQAEGPRN
                     KLAERGWQVAVYAGVVAALWWSAHYVLGTLSLGDVGQALGLAVLTLIRVVVLIVLASF
                     IWVPLGVTIGLRPKLAESVQPVAQFLAAFPANVLFPFAVIVIVRFHLMPDIWLSPLMI
                     LGTQWYILFNVIAGASAIPSDLREAASMFGVKGWQWWRQVALPAIFPYYITGALTASG
                     GSWNASIVAEAVSWGQSHLEAHGLGAYIADATTAGNYPQVALGIGMMSLFVLGFNRLV
                     WRPLYAYGERRLRIG"
     gene            4736..6046
                     /locus_tag="VFF98_10820"
     CDS             4736..6046
                     /locus_tag="VFF98_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZU64168.1"
                     /translation="MATIAPLAPLVEVEGLNHYYGPPPGGHLVLEGVNLTIGQNEVVG
                     LLGRSGSGKSTLLRSIAGLIHPREGEVRFVPTEDCPDPSVSMVFQTFALFPWLTVQEN
                     VELGLEAQKVPAEERRARALAAIDLIGLDGFENAYPKELSGGMRQRVGLARALVVNPS
                     LLLMDEPFSALDVLTAETLRTDLLDLWSEGRMPIKSILIVTHNIEEAVQMCDRILVFS
                     SNPGRVVADIKVDIPRPRDRLDPPFRALVDDIYARMTARAAPESPLRDGLFPGTGIAM
                     VLPPKVSTNALAGLIEEVDGTPFNGKASLAELADSLQLEVDELFPMAETLQLLRFAEL
                     RGADLVLTVAARRYAQSDVDQRKAIFAQALLAYVPLAQHIRRVLDERASHRAPARRFR
                     DELEDYMSEDYADETLRTVTHWGRYAEIFTYEEDDDEFSLENPQ"
     gene            6163..8220
                     /locus_tag="VFF98_10825"
     CDS             6163..8220
                     /locus_tag="VFF98_10825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZU64169.1"
                     /translation="MFSSRTAIAVSALALALGSTPLGAKPAPAKAPPASPAHAAAPAP
                     QGFGAWGVDLATRDLSVRPGDDFQRYASGKWLDSTAIPADRASTGTFYDLRETVQAQV
                     RDIITTAPPAGPIGALYTSYMDAAHREAIGLAPLMADIASLRALANRADLARFMGTSR
                     SRFGISLVEAGLMPDTANAEMNVLGLGQSGLGLPDRDYYLKDQFKPQREAYRAYIART
                     LAAIGEADAPAKADAVLAFETEIARLSWDNARRRNLDLLNNPYSTAELAAYAPGIDWD
                     AYFAGAGIAPQKRIIVAENSAIQAISALYAATALDTLKLWEAFHVADQAAPYLTKAMD
                     DSRFAFTKTLSGVAVQRPQWKRGVDLVSGQLGELVGQTYVARHFSPAAKAKMEGLVAH
                     LKEAMAGRIRANGWMSEATKQAALLKLSRMDVMVGYPDKWRDYAGLAMAPGDLYGNVE
                     RASRFNSDYDLADLGKPVDHRKWAMSPQTVNAYNGEQENKIVFPAAILQPPFFDPHAD
                     DAVNYGAIGAVIGHEISHAFDDQGRKIDASGTLRDWWAPEDAKRFEAEAKVFGDQYAR
                     FEAAPGAFVNPGLTMGENIADFAGVEVALDAYHAALGAKAPPARDGLTGDQRFFLAFA
                     QVWRAKSREDALRNQVATDPHSPARFRVLGPLRNVDAWYQAFHVQPGDKMYLPPEKRA
                     RIW"
     gene            complement(8226..8921)
                     /locus_tag="VFF98_10830"
     CDS             complement(8226..8921)
                     /locus_tag="VFF98_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZU64170.1"
                     /translation="MKTALIIRHVPYEGVAGFRAPIEAAGYVVDRIDVADPAFSSLDL
                     AEPDLLIMMGGPMGVYESDRHPWIACQLRRLQRRLAADRPTLGVCFGAQMIAAALGAK
                     VYKGPAKEVGFHPVQLRAEAEGGPLRHIAEVPVLHWHGDTFTRPEGVELLASSALYPH
                     QAFCRGETVLALQFHAEMGLDPRFDAWVAQGPGDIAEAGHTEATLRAAHDTHGATAVA
                     AGQAMIGEWLAGL"
     gene            complement(8966..9886)
                     /gene="cysK"
                     /locus_tag="VFF98_10835"
     CDS             complement(8966..9886)
                     /gene="cysK"
                     /locus_tag="VFF98_10835"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZU64171.1"
                     /translation="MKAESVLSTIGGTPHIRLSRLFPDHEVWVKSERGNPGGSIKDRI
                     ALAMVEAAEADGTLQPGGTIIEPTSGNTGIGLALVAAVKGYKLVLVMPESMSLERRRL
                     MLAYGATFDLTPREKGMKGAIERAKELAAETPGAWIPQQFENPANIDVHVRTTAHEIL
                     DDFADTPIDYVITGVGTGGHLTGTAETLKASWPALKAFAVEPALSPVISGGQPAPHPI
                     QGIGAGFVPANLHTQSIDGVIQVDAAQAKEWARRSAREEGLLVGISSGATLAAIAQKL
                     AEIPAGSRVLGFNYDTGERYLSVADFLPEL"
     gene            complement(9876..>10430)
                     /locus_tag="VFF98_10840"
     CDS             complement(9876..>10430)
                     /locus_tag="VFF98_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU64172.1"
                     /translation="DLGAWDKPRRNLWASRLGLAIRAAGRPVVLAAHSLGCHAVAWWA
                     RMEPAAAALVRGALLVAPPDVDEAPVDARLRGFAGSAGQALPFRATLVASSDDPYLSL
                     AGARVLAQRWGAQFVDIGARGHINAESRLDVWPRGQALLDDLLGEAAGPAYDTSLAGP
                     SAFPQLSVSTPFFVPHKEASIHEG"
BASE COUNT         1656 a         3677 c         3371 g         1726 t
ORIGIN      
        1 cgaacagggg tgggcaacgg cggtgggcac cgcgcggccg gccgcggcat gcaccagccg
       61 gcgataggca tcatgctcgg ccaggcggat ctcgggcagg ctgacagccg ggcggcgcag
      121 cttctcctgc ggcgcgatca catcggcctc gccctcgatc accgtctcgc cctgctggtt
      181 ggcgcagctg caggccagcg tcagccggcc cttctcgcgc ttgtcggcca cacgcacgct
      241 ggcggtgatg gtatcgccca gccccaccgg gcgcaggaac ttcagcgttt ggcccagata
      301 gatcgtgccc ggccccggca gatgggtgcc cagcaccgcg ctgatcagcc ctgcccccca
      361 caatccgtgg gcgatcacct tgcggaacat gtcggtggcg gcataggccg ggtccatatg
      421 ggccgggttc acatcgcccg acaccagcgc gaacagctcg atatcctgat gcgtcagcgt
      481 gcgggtgagg ctggcgctgt cgccaacgga tatctcctca taggtgcggt tttcgatcat
      541 gggcgtggcg gcttcgggcg aatcggcgga aagcagcgtc atgcggggcc tgcggggctg
      601 tggtcgatcc gcttgatata gcagcttatg cgagcagaaa catgacgaag cgcaagcgcc
      661 aattgacaag gcccacgccc accaatatca gaaccataat tccaaattta tccaaaacag
      721 gggagagaga agatcatgcc cgccgcccgc cttgcggccg cgctggggct ggccatggcc
      781 agccaggcgc ccgccgccct tgcgcaaagc tgccacccgc tggccggcca ctggcgctat
      841 gatgccgctg ccagccatgt cggctcgggc ctctcgttca acccctatta cgcggtgagc
      901 ggcatcgatc tggtgctgga acagggcgcc ggcggcctca cccagagctg gcacctcacc
      961 ggcccccacc ttgacgagac cgaccgctac accacgcccg ccgatggcac gctggcgccc
     1021 accggcaccc gctcggcgct caatgccgtg ccgcaggccg tgagcggcca gtgggaaagc
     1081 tgcacgctgg tggaggaagg ccgcgccagc ctgttcggcc agaccgtctg gacccgcagc
     1141 cgcttcttcg tggcggcaga caaccgccgg ctcaccatca cccaatcctc ccattcagac
     1201 cttggcgatg tggaacgcac gctgatcttc acccgcaccg acgagaggcc cctgccatga
     1261 gccctctgcc cctcacccgc cgcgcgctga tcggcgcggc cgcgctggct ccgctggcca
     1321 cgcaggccgc cgccgccacc cggcccgcca cggcgatcac ccttgatccc catgcgccca
     1381 ccaccccctt cccgcattac tgggaagcct gcgtcggctc cgaccgggcg gcagtggcga
     1441 tgcgcgccca gtggcagcag gatctggcgc tggcccggca cgaggcaggc ttccgctcgg
     1501 tgcgcttcca tggcctgttc gacgacgaga tgggcctgtg ccgcagcatc ggccccaacg
     1561 ggccggaact gaacttcctc tacatcgacc agatcttcga tgccatgctg gccatcggcg
     1621 tgaagcccta tgtggagctg agcttcatgc cgctgccgct ggcctctgat ggcaacacca
     1681 tcttctggta caagggcaac acctcgccgc caaaggacat ggcgctgtgg gtgcaggtga
     1741 tcgaggggct cgcgcggcat ctgctggccc gctatggcgc ggccgaggtg acgggctggc
     1801 gctttgaagt gtggaacgag cccaacctca acttctgggc cggcacgcaa ggccagtatt
     1861 tccagctcta tgccgccacc gcgcgggcgc tgaagcgagt gaacccggcc ttgcaggtgg
     1921 gcgggccggc cactgcccag ctgatgtgga tcgacgattt cctgcgcttt tgcgcggccg
     1981 acaacgcccc tgtcgatttc gtctccagcc atatctatgc cgatgatccg caggaaaacc
     2041 tgttcggcca ggatctgggc ctgccgcgcg aacaggtgat cccgcgcgcg ctgcaagtgg
     2101 ccaatggcaa gatccgcgcc tcggcgtttc cgcgcatccc gctggtgatc tcggaatgga
     2161 ccagccagaa ccccgccttc atcgcccaga ccatccgcga ttgcgccggc ctgtgcgaca
     2221 ccttcagcta ctggaccttc gacaatatct tcgaggaaca cgcgccgatc ccggggttcg
     2281 gcaacacgct gtatggcctc atcggccaga gcggggtggc gcgcccctcc ttccgtgcct
     2341 tccagttgct gcaccggctg ggcacgcagc ggatcgacac cggcagcgcg cccctcatcg
     2401 ccacccgcag ggaagatggc acgctggcca tccttgcctg gaacctgatc accaacgccg
     2461 atgtctcggg ccagacgggc aatccgatga acgcgccggg cgaggagccg atcctccatg
     2521 gccccccgcg cgatctgctg ctgcggctgg gcggcggcgc gcgcaggcgc gcgcgcgtca
     2581 ccggggtgga tctcgccgca gatccggtga cgctggccta tgccgccatg ggccggcccg
     2641 cctcgcccac cgccgcccag atcaccgccc tgcgcaaggc cggcgcgctg ccccaggccc
     2701 ggccgctggc cggcgggccg ggcggcttcc atctggcgct gccagccaac gccctggcgc
     2761 tgatcgagat cggctgagcc acgcccgcgc tgtcacacag ctgtctttga gcccggtcta
     2821 tggcgatgcc atggccgggc gtgcccgcgc cgcagcggaa tttgcgcttg caaccgcagg
     2881 cgcatatccg gcagcggcgg ttctgccccg aatcggacac atcactcgga aagacattgc
     2941 ggcagacgac acctatgagc ccggaaaaga accccgcccg cgccttgccg gccttctcgc
     3001 gcatgttgcg cctcacccgg cccaaccgcg aggatgcgat cgcctttgtc attctggcgg
     3061 ggctggcgct ggtggtgttc cgcggcgccc agggcgcggt ggagccgctc tcgcgcctgc
     3121 atctggaacc gctgctgctg gatccgggcc agctgcccta ttatgccctg cgcaccatcc
     3181 tgcgcatgtt tgccgcattg ttcgcctcca ccgtcttcac gctggtggtg gcaacgctgg
     3241 ccgcgcgcag ccggcatctg ggccagatca tcgtgccggc gctggatatc ctgcagtcgg
     3301 tgccgatcct gggcttcctc accttcacgg tcactttctt catggggctg tttcccggca
     3361 atgtgctggg ggtggaactg gcgagcgtgt tcgccatctt cacctcgcag gcgtggaaca
     3421 tggccttcag cttctaccag tcgctgcgca gcctgccgtc tgacctggag gaagtgtgcc
     3481 agagcttcgc gctctcgccg gtgcgccgct tcctcaagct ggacctgccc tttgccacgc
     3541 ccgcactggt gtggaacgcg atgatgtcga tgtcgggcgg ctggttcttc gtggtcgcct
     3601 ccgaggcgat caccgtgggc aacaccaccg tcaccctgcc gggcatcggc tcgtggctgg
     3661 cactggcgat cgagaagcag gatttcaccg ccgtggccga agcggtgggc accatggccg
     3721 tggtcatcgt ggcctacgac cagctgttct tccgcccgat cgtcgcctgg gcagaccgct
     3781 tccgcttcga acagaccgcc agcaccgagc ggcccgaaag ctgggtctat gacatgctgc
     3841 gccgcacccg cctgctgccg ctgctgtttg cccccgtcag cgccatcggc cgcctgctgc
     3901 tggcgctgga gccggcccgc cgcgcccgcc gcctgcaggc cgaaggcccg cgcaacaagc
     3961 tcgcagagcg tggctggcag gtggccgtct atgccggcgt ggtggccgcg ctgtggtggt
     4021 ccgcccatta tgtgctgggc acgctgagcc ttggcgatgt cgggcaggcg ctggggctgg
     4081 cggtgctcac gctcatccgc gtggtggtgc tgatcgtgct ggccagcttc atctgggtgc
     4141 cgctgggcgt caccatcggg ctcaggccaa agctggccga aagtgtgcag ccggtggccc
     4201 agttcctggc cgccttcccg gccaacgtgc tgtttccctt cgcggtgatc gtcatcgtgc
     4261 gcttccacct catgcccgat atctggctct cgccgctgat gatcctgggc acgcagtggt
     4321 acattctgtt caacgtcatc gccggcgcct cggccatccc ttcagacctg cgcgaggcgg
     4381 ccagcatgtt cggcgtgaag ggctggcagt ggtggcggca agtggccctg ccggcgatct
     4441 tcccctacta catcaccggc gcgctcacgg cctctggcgg ctcgtggaac gcctcgatcg
     4501 tggcagaagc ggtcagctgg gggcaaagcc atctggaagc ccacggcctt ggcgcctata
     4561 ttgccgatgc caccacggcg ggcaattatc cgcaggtggc gctgggcatc ggcatgatga
     4621 gcctgttcgt gctgggcttc aaccggctgg tgtggcgccc gctctacgcc tatggcgagc
     4681 gccgcctgcg catcggctga cctctttttt cttcctgctg accggagcct ttttcatggc
     4741 caccatcgcc ccgcttgccc cgctcgtcga agtggaaggc ctcaaccact actacggccc
     4801 cccgccgggc ggccatctgg tgctggaagg ggtgaacctt accatcggcc agaacgaggt
     4861 ggtgggcctg ctcggccgtt cgggctcggg caagtccacg ctgctgcgct ccatcgccgg
     4921 gctgatccac ccgcgcgagg gtgaggtgcg cttcgtgccg accgaggatt gccccgatcc
     4981 cagcgtgtcg atggtgttcc agaccttcgc cctgttcccc tggctgacgg tgcaggaaaa
     5041 tgtggaactg ggcctggaag cccagaaagt gccggccgag gaacgccgcg cccgcgccct
     5101 ggccgcgatc gacctcatcg gcctcgacgg cttcgagaac gcctatccca aggaactgtc
     5161 gggcggcatg cgccagcggg tggggctggc gcgcgcgctg gtggtgaacc cctcgctgct
     5221 gctgatggac gagcccttct ccgcgctcga cgtgctgacc gccgaaaccc tgcgcaccga
     5281 cctgctcgac ctgtggtcgg aagggcgcat gccgatcaag tcgatcctca tcgtcaccca
     5341 caatatcgag gaagcggtgc agatgtgcga tcgcatcctc gtcttctcgt ccaatccggg
     5401 ccgggtggtg gccgatatca aggtggatat cccccgcccg cgggaccggc tggacccgcc
     5461 cttccgcgcg ctggtggacg atatctatgc ccgcatgacc gcccgcgccg cccccgaaag
     5521 cccgctgcgc gatggcctgt tccccggcac cggcattgcc atggtgctgc cgcccaaggt
     5581 ttccaccaac gcgctggcgg gcctgatcga ggaggtggat ggcaccccct tcaacggcaa
     5641 ggccagcctg gcggaactgg ccgattcgct gcagctggaa gtggacgaac tgttccccat
     5701 ggccgaaacg ctgcaattgc tgcgctttgc cgaattgcgc ggggccgatc tggtgctcac
     5761 tgtggccgcc cgccgctatg cccagtctga tgtggaccag cgcaaggcga tctttgccca
     5821 ggcgctgctg gcctatgtgc cgctggccca gcatatccgc cgcgtgctgg atgaacgcgc
     5881 cagccaccgc gcacccgccc gccgcttccg cgatgagctg gaggattaca tgtcggaaga
     5941 ctatgccgac gagaccctgc gcacggtgac gcattggggc cgctatgccg aaatcttcac
     6001 ctatgaagag gacgacgacg aattcagcct tgaaaatccg caataagctc catttgccgc
     6061 ggcttggttg acaggcgctg ccgcgggcgc aatgcaggca tcccctgtgc ccatggcgcc
     6121 cgcaagggcg cccggccacc gccaaggatg cctgccgccg tgatgttttc cagccgcacc
     6181 gccattgccg tttccgccct tgcgctggcg ctgggcagca ccccgctggg ggcaaaaccc
     6241 gcccccgcga aggcgccccc ggcaagccct gcgcatgccg cagcgcccgc gccgcagggc
     6301 tttggcgctt ggggggtgga tctggccacg cgcgatctgt cggtgcggcc gggcgatgat
     6361 ttccagcgct atgcctcggg caaatggctg gatagcaccg ccatcccggc cgaccgcgct
     6421 tccaccggca ccttctacga cctgcgcgaa accgtgcagg cccaggtgcg cgatatcatc
     6481 accaccgccc cgcccgccgg cccgatcggc gcgctctaca ccagctatat ggatgccgcc
     6541 caccgcgagg cgatcggcct ggccccgctg atggccgata tcgccagcct gcgcgcgctg
     6601 gccaaccggg cggatctggc ccgcttcatg ggcaccagcc gcagccgctt cggcatctcg
     6661 ctggtggaag ccggcctgat gcccgacacc gccaatgccg agatgaacgt gctgggcctt
     6721 ggccagagcg gccttggcct gcccgaccgc gactattacc tgaaggacca gttcaagccc
     6781 cagcgcgagg cctatcgcgc ctatatcgcc cgcacgctgg cggccatcgg cgaggcagac
     6841 gcgcccgcca aggccgatgc cgtgctggcc ttcgagaccg agatcgcccg gctgagctgg
     6901 gacaatgccc gccgccgcaa ccttgatctt ctgaacaacc cctattccac cgccgagctt
     6961 gccgcctatg ccccggggat agactgggac gcctatttcg ccggcgccgg catcgccccg
     7021 caaaagcgca tcatcgtcgc cgaaaacagc gccatccagg cgatctcggc gctttatgcg
     7081 gccaccgcgc tcgatacgct gaagctgtgg gaggctttcc atgtggccga tcaggccgcg
     7141 ccctatctca ccaaggcgat ggacgacagc cgctttgcct tcaccaaaac gctatcgggc
     7201 gtggcggtgc agcggccgca atggaagcgc ggggtcgatc tcgtctcggg ccagctgggc
     7261 gagctggtgg ggcagaccta tgtggcgcgc catttctcgc cggcggccaa ggccaagatg
     7321 gaagggctgg tggcccatct gaaagaggcg atggccggcc gcatccgcgc caatggctgg
     7381 atgagcgagg ccaccaaaca ggccgccctg ctgaagctgt cgcgcatgga cgtgatggtg
     7441 ggctaccccg acaaatggcg cgattatgcc gggctggcga tggcgccggg cgatctttac
     7501 ggcaatgtgg aaagggcaag ccgcttcaac tcggattacg atctggcgga tctgggcaag
     7561 ccggtcgatc accggaaatg ggcgatgagc ccgcagacgg tgaacgccta taatggcgag
     7621 caggaaaaca agatcgtctt ccccgccgcc atcctgcagc cgcccttctt cgatccccat
     7681 gccgatgatg cggtgaatta tggcgcgatc ggcgcggtga tcggccatga gatcagccat
     7741 gccttcgacg atcagggccg caagatcgac gcctccggca ccctgcgcga ctggtgggcg
     7801 cccgaggatg ccaagcgctt cgaggccgag gccaaggtgt ttggcgacca atacgcccgt
     7861 tttgaggcag cgcccggcgc tttcgtcaac cccggcctga cgatgggcga gaacatcgcc
     7921 gatttcgccg gggtggaagt ggcgctcgat gcctatcacg cggcactggg ggcaaaggcg
     7981 ccccctgccc gcgatggcct cacgggtgat cagcgcttct tcctcgcctt cgcgcaggtg
     8041 tggcgggcca aatcgcgcga ggatgccctg cgcaaccagg tagcgacaga cccgcacagc
     8101 ccggcgcgct ttcgcgtgct ggggccgctg cgcaatgtcg atgcgtggta tcaggccttc
     8161 cacgtgcagc cgggcgacaa aatgtatctg ccgcccgaga agcgcgcacg gatctggtaa
     8221 ggggctcaga gccccgccag ccactcgccg atcatcgcct ggcctgcggc caccgccgtt
     8281 gcgccatgcg tgtcatgcgc ggcgcgcagg gtggcctcgg tgtggccggc ttcggcgata
     8341 tcgccgggcc cctgcgccac ccaggcgtca aaccgcggat ccagccccat ttcggcgtgg
     8401 aactgcaggg ccagcaccgt ctcgccgcgg cagaaggcct gatgcggata gagcgcgctg
     8461 gaagccagca gctccacccc ctcgggccgg gtgaaagtgt cgccatgcca gtgcagcacc
     8521 ggcacctcgg cgatatggcg cagcggcccg ccctccgcct cggcgcgcag ctgcaccggg
     8581 tgaaagccca cttccttggc cggccccttg tagaccttgg cccccagcgc tgcggcgatc
     8641 atctgggcgc cgaaacacac gcccagcgtt ggcctgtccg ctgccaggcg gcgctgcaaa
     8701 cggcgcagct ggcaggcgat ccaggggtgg cggtcgctct catagacgcc catcggcccg
     8761 cccatcatga tgagcagatc cggctctgcc agatcgaggc tggaaaaggc gggatctgcc
     8821 acatcgatcc ggtcgaccac atagccggcg gcttcgatag gggcgcgaaa gcccgccacg
     8881 ccctcatagg gaacgtggcg gatgatgaga gcggtcttca tggttgcagg ctaacacata
     8941 tgaagggggc agatgccccc gattgttaca gttcgggcag gaaatccgcc accgacagat
     9001 aacgttcgcc ggtgtcgtaa ttaaagccca gcacgcggct gccggcggga atttcggcca
     9061 gcttctgcgc aatggcggcc agcgtcgcgc ccgaggaaat gccgaccagc agcccttcct
     9121 cgcgggcgct ccggcgggcc cattccttgg cctgggcggc atccacctgg atcaccccgt
     9181 cgatcgactg ggtgtgcagg ttggccggca cgaagcccgc gccaatgccc tggatcgggt
     9241 gcggcgcagg ctggccgccg ctgatgacgg gcgacagcgc cggctccacc gcaaaagcct
     9301 tcagcgccgg ccagctggcc ttcagcgttt cggccgtgcc ggtgaggtgg ccgccggtgc
     9361 caacgccggt gatcacgtaa tcgatcggcg tatcggcgaa atcatcgagg atctcatgcg
     9421 cggtggtgcg cacatgcaca tcgatgttgg ccgggttctc gaactgctgc gggatccacg
     9481 cgccgggcgt ttcggcggcc agttccttcg cgcgctcgat cgcgcccttc atgcccttct
     9541 cgcgcggggt gagatcgaaa gtggcgccat aggccagcat cagccggcgg cgctccaggc
     9601 tcatcgattc cggcatgaca agcaccagct tgtagccctt caccgccgcc accagggcca
     9661 ggccaatgcc ggtgttgccc gaggtcggct cgatgatcgt gccgcccggc tgcagcgtgc
     9721 catcggcttc ggccgcttcc accatggcaa gggcaatgcg atccttgatc gagccgcccg
     9781 gattgccgcg ttcgcttttg acccacactt cgtgatcggg aaacagccgc gacagccgga
     9841 tatgcggcgt gcccccgatg gtcgacagaa cagactcagc cttcatggat cgatgcttcc
     9901 ttgtgtggaa cgaaaaacgg cgtggaaacg gacaattgcg ggaaagcgga agggccggca
     9961 agcgatgtgt cataggccgg ccccgccgcc tcgcccagca gatcatccag cagggcctgc
    10021 ccgcgcggcc acacatccag ccggctttcg gcgttgatat gcccgcgcgc gccgatatcg
    10081 acaaactgcg ccccccagcg ctgcgccagc acccgcgcac cggccagcga gagataggga
    10141 tcatccgagc tggcgaccag cgtggcgcga aacggcagcg cctgcccggc agagccggca
    10201 aaaccgcgca gccgcgcatc caccggggcc tcgtccacat cgggcggggc caccagcaga
    10261 gcgccgcgca ccagggcggc agcggccggc tccatccgcg cccaccacgc caccgcatgg
    10321 cagcccaggc tgtgcgcggc cagcaccacc ggccggccgg cggcgcgaat cgccaggccc
    10381 agccggctgg cccacaggtt gcggcggggc ttgtcccatg cgcccagatc
//
LOCUS       DASRYE010000214        15090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_207123,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000214 DASRYE010000000
VERSION     DASRYE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207123"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFF98_10845"
     CDS             <1..292
                     /locus_tag="VFF98_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64173.1"
                     /translation="LARLEPALGPLLHHMPRLRAIGRIRNAACDGEMGAAGSRLARDW
                     AIAAGGGQGMAAGAPFTRIDAAALAQIAQFYLALADQLRSAFARTRGGLDFS"
     gene            complement(686..1951)
                     /locus_tag="VFF98_10850"
     CDS             complement(686..1951)
                     /locus_tag="VFF98_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64174.1"
                     /translation="MGARWAGRHPAIGVAAVTFLALLVAAGVRSAPSVMMVPLQLHFG
                     WSRAEVSLTSAIGIFLYGLVGPFAAALMLSFGIRRTMLLGLMLMALATLASLGMTRSW
                     HYLLSWGVISGLGSGAVASVLAAAVVGRWFAQRQGLMMGLLSASTATGSLVFLPVLAW
                     LSGPARWQPVAQAVGLACLAVVPLVLAFVPEHPADAGVRRFGEPDDAPLPPRAAAGVG
                     LALRVLARAVRVPLFWLLFGTFFICGLTTNGLVGTHLIAYCGDHGIAPVAAAGLLSTM
                     GLFDLVGTTASGWLTDRYDPARLLVAYYALRGLSLMALPFVPFSPLPLAVFAVFYGLD
                     WIATVPPTVAMAVRRFGAREAPIVFGWVVVGHQIGAAVAAFAGGLLRQDWGSYAPAFV
                     VAGALGVGAGLAMAAHAMGARRGLVPAAG"
     gene            complement(1954..3693)
                     /locus_tag="VFF98_10855"
     CDS             complement(1954..3693)
                     /locus_tag="VFF98_10855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU64175.1"
                     /translation="MRESLGWVAHSSEVLKTANRSLDHLRAAESGLRGFVLTHDPVFA
                     ETADAEIAAARQGAAGIVQLTQDNPSQNSRARQIRALIDDRAASLQQSAGLARKGDFA
                     GAQANIASGRGRYLMVLVDARVGDLVEEERALAGTRMATATYRLDAIGWLVLLGTPVT
                     MLLVLGACIVLSGRIRQPVEAMLAVMGQLGRGDRSARIDTAMRSSEFERLAVGYNAMA
                     GELAAAVADQVDSEERLRLANLELSRNAEVLRERGEVIELLGGMAHRMQAARTDEELA
                     AIIRVFVPRVLPDIPGVLYAHNNSRNLLVPIAAWGGLEVEAVGFPPDQCWALRRGQSH
                     FVIEPGSDIVCAHAVDVTDHYHCEPLLAGGEVIGVLYLRGVVEAENRFRLTVLTENIA
                     SALVNHRLQRGLREQTIRDPLTGLFNRRYMEETLALEIARVLRSGGPLSLVMCDVDHF
                     KRFNDEFGHDAGDAVLQAVAAEMRSRFRDGDVICRFGGEEFTIIAPGSTADVLARRVE
                     VVRQAISELTVHQGGRTLGAITMSFGIASWEAHMERDGSTLITAADASLYQAKREGRN
                     RVAVDLRAEPADA"
     gene            4043..5086
                     /locus_tag="VFF98_10860"
     CDS             4043..5086
                     /locus_tag="VFF98_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64176.1"
                     /translation="MNATSRETPHFDVARAAQVAEAYAGSIDPIFSLGTQPLDGPGAD
                     VGLDAYHAGAMLIGRAHMHGARFDYARDRAKIARTGLDMILVQIITAGSDVRFHGDEV
                     QVTRPGDICIDDLTRPFATRATDCENINIILPRAALGLDALQLDRIHGLSFAADSLAA
                     RLVSAQANALLDRFDSCSPGEAAAAARATGLMIGGLAAPLANQREAEEAISVAVLHRV
                     QRYIQAHLARPDLTPDGIARAVGLSRASLYRIFSPIGGVREYIRQQRLGRVFADLRNP
                     RLRARSIAEMAYGWGFADWSSFSRAFKAQFAVTPSEVRGLAGVLAPADSAGIVLPQWL
                     REMEAGEIGPAMA"
     gene            complement(5109..6716)
                     /locus_tag="VFF98_10865"
     CDS             complement(5109..6716)
                     /locus_tag="VFF98_10865"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HZU64177.1"
                     /translation="MDIFASLFAGLGLFFIGIRLIGSNLKQMAGRKLRQLITRAVAGP
                     VSTAVFGLLAGAIMQSVNAVTYVLVALVSAGTIEKRRAFPVVSWANLGTSMLVLIAAL
                     NMHLAILTLLGMTGAAYYLGLDQSSRHRHVVNALLGVGLLFLGVDFIKTAAHGLRDSS
                     LLHVAMAYSGRFWVLSFLVGAAVAMVIQSSTTVAVLAMAMAGAGLLPFLSGAMVVMGA
                     GLGSGLSALPLAGKLKGSARQLVLYQLALKAVGVAGLLGLFLADWLAGGRIIAPAFGF
                     LHLSRSGELAGVYLALQLASTACMALVEGPIARRIDALSPPSVEEVLGRPHYLSEDAL
                     ADAGTAILVAEREQARLLGSLPDYLAALREEGGAGLLPVAARLEADGSVTRECNMFLA
                     ALADSYDDREVREQVMRLRDRNELIAALQNSLFELHESVGGADDRGVLSLMHSLVESL
                     HMVLETLADVGSSADAEDLELLRALTHDRSELMDGIRRRMQGSALPPDAQQRAFIATT
                     IFERCMWLLRRYVLQLQGQQAPQGSAG"
     gene            7035..8543
                     /locus_tag="VFF98_10870"
     CDS             7035..8543
                     /locus_tag="VFF98_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64178.1"
                     /translation="MTSLPVPGSARPDAAHKGNSAGALARRPASARPASAKLASLALL
                     ASAGAPVALHAQEIASPAPADAPLPADASLPADSGPAASPGEAHGLKALRLSGFATLG
                     FTYNDNSHAGVITSYAQAHPANSGFSADLDSVVGVQAVWTPLKGTSITVQLVARPDNG
                     MKPAVRMAYLRHELTPSLAVRGGRIRSPLYFDSDVSEVGYAYLTARPPIPLYTIVNSV
                     DTLDGGDVQWRHSWHDVAVLLQAYYGNSQYRHHFYNDGFAAHAKLTGIKGGAVSLILP
                     WITLRASRTWISSYTMRGGAIDTLDAGLETLATQMNAAAANPYLPAGYGAALGAQASA
                     ISALMYPFDNHPIYTSVGFDASFNKLRVLGEWAELDSRSRMVGRYQGYQVTVGYALGN
                     VTPYATYARQRRLGPALDTSALNATGLSEQLDAGLAQMQQALALAGSYANLSGSSLSL
                     GARWDVREHVAIKAQYDRLETPASTIPGTFAVSALPIRNRANLATVNLNLVF"
     gene            8556..8993
                     /locus_tag="VFF98_10875"
     CDS             8556..8993
                     /locus_tag="VFF98_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64179.1"
                     /translation="MKTPAALMAALIAAMALPGAAHAQDSVVVIANKSVPLDRLDNQR
                     ATQIFLKQIQTWPDGKPIAPVDLKEGAPLRAEFYAKVTGRSAGQVRAYWARQEFTGMG
                     FPPRQVASPADVTRFVQATPGAVGYVNRKAADGSVKTLLNVSR"
     gene            8990..11476
                     /locus_tag="VFF98_10880"
     CDS             8990..11476
                     /locus_tag="VFF98_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZU64180.1"
                     /translation="MSTAAPGAEARQGAGSGLFAPTVWLSARLSFSRKYMVIGLLVMI
                     ALAALSTPLLLQINRQIETAARERVGLGHVAAQTDLLAGLVRRRSALLGNDRLPDPAA
                     LDRAVRQAVRIGRADGLEADAARLEQGWQASQGELGEPQVRFAAATGAINAALALIRD
                     EARLYRLNVDPDLDAALEMLSLRLPLVLDIIGKQRDALAIRQADITSYALSAQVALTE
                     SAPVLRAGVNQLVAAGADPALGPQLSRLLADIARQQDSVDRSVGAAANREELQQIAQG
                     NIASAQGLLAKAASAVDSRLRQRIEALSRERAVILALILGELGVVFYLFAGIYISTLQ
                     SLKRLSHGTRAFCSGRMDARIQIDTHDELVLIARNFNTMASEVERLLGVIQQQNESRQ
                     AELESEVRLRTAELAERNRELQRAGKRVAEELDLARSMQKAILPQTFPDEAGWGVHAA
                     MHPARELGGDFYDCVPLPGGRYGVLVADVAGKGVGAAFFMAVARTVLLDLALSGLSPE
                     AVLARANDLLCERNPVELFVTTCYAVFDPGDGSLVYASAGHHPPLVRRADGRAQTLAS
                     ANEVALGVMPQMAYSLARAQLAPGEAVLLYTDGITEAFDAAGDPYGDARLAAWFERCA
                     PDAPAADLVAGLVADVARFVGGEEASDDLTCLVLCRKSGELPMQDPPKPASVPALPVE
                     IGDKRQLLHRLIDSRVEEIAGLAEAIEEALPDRADLAFVANLCLEELITNTILHGFAG
                     QPGHAIEVFLNRSEEWLEIILKDDAPPFDPFVEAPAPDIEADLDGRAVGGLGVYIVKT
                     MMDAAHAYYDGGGNLIVLLKALQQPESPTQ"
     gene            11505..11849
                     /locus_tag="VFF98_10885"
     CDS             11505..11849
                     /locus_tag="VFF98_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZU64181.1"
                     /translation="MSFEAQLNVEHAINWLSLSGRLDSATANDLEQVLLPLFAAPAPT
                     VLVDFAGLGYISSAGLRVVLMAAKRAKQAGGRLMLCGLSSAVREVFEISGFLKILEVS
                     DSRAEAERLLLA"
     gene            complement(11854..13215)
                     /locus_tag="VFF98_10890"
     CDS             complement(11854..13215)
                     /locus_tag="VFF98_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU64182.1"
                     /translation="MSIEATPLSALRSGAFRFALALALVFALSSLGLLWMMDRQVAAY
                     GQEATEAMLHAEARVLAGEYAQTGREGLLNALQRHSASTREQPFHYLLQDAQGRRLFG
                     DIPAGLGRPGAATITLAEPDSGPVAMRQFGMRIGDGQLLVVATDFFDIENLRHRLARY
                     TLLGAVAISLCALFGGYGVGRLFLRRLDGVNRQVERIVAGNGAERLPAIGLAPEFDTL
                     TRNLNHMLERQDAAMEGLRQVSTAIAHDLRTPLTRLHQRLESLNARLDADAGVELAIG
                     EVEGLLSTFQALLRIGMIEGGMGRGRFQPVDLSEELARIVETYAPVAEDAGHRLGASL
                     AAGLWVEGDAELLTQLFINLVENAIVHTPVGTAIHIALAARGDRVCATVSDDGPGVDP
                     ADHARIFRRFYRCAASKGLPGTGLGLSLVAAIADMHGAACTLLPADRGFALELAFPRW
                     RGG"
     gene            complement(13219..13893)
                     /locus_tag="VFF98_10895"
     CDS             complement(13219..13893)
                     /locus_tag="VFF98_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU64183.1"
                     /translation="MKILLLEDDAVLSEHLVRVLGEAGHTVDTCRAGQDAIYLASTQD
                     YGVLILDRMVEGIDGLAALKALRAMGVRAPALFLTAMTGVDDRVDGLDAGGDDYLAKP
                     FAAREFLARVHALGRRVPLADVPSALCIGDIEIDRLKRSVTRGGQALRLQAQEYKLLE
                     YLMLHAGQVVTRSMLLENVWSYHFDTRTNIIESHMSRLRAKLCETGRPDPIQTLRGAG
                     YRFHEQ"
     gene            14048..>15090
                     /locus_tag="VFF98_10900"
     CDS             14048..>15090
                     /locus_tag="VFF98_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU64184.1"
                     /translation="MPVFMPCLPARLPAPRLPVPSGAPAARKGRWLAAAGCALLAGCA
                     HYAPLPLPARPALAPGVAALAQPPASERPLGIAEVAALALANNPDLKAVRLRDAVARG
                     QTRQAGLLPNPQVTGAFLPLLSGVGTVSAWNAGLAQNLRAIVTYRAHLRAAQDSQQQV
                     AADIVWQEWQVAGQARQLAADIIMGEQARPAYAETLALLADRNARLEQAMAQGQATLA
                     QLAPDRAALQAARAALDAQDQRQLALRQQLNALLGLRPDAPLPLVAEPDLPPFAPAAL
                     RAGLASLPDRRPDLIALRCGYAAADEGVREAIRAQFPDLVLGGGIASDNSKVISGGPN
                     AALGLPVFDRNQG"
BASE COUNT         2509 a         5487 c         4867 g         2227 t
ORIGIN      
        1 gctggcccgg ctggagccgg cgctgggccc cctgctgcac cacatgccgc gcctgcgggc
       61 gatcgggcgg atccgcaacg cggcctgcga tggcgagatg ggcgcggccg gcagccggct
      121 ggcgcgcgat tgggccatcg ctgccggggg cggccagggc atggcggccg gcgccccctt
      181 cacccggatc gatgcggccg cactggcgca gattgcccaa ttctacctcg cccttgccga
      241 tcaattgcgc agcgcttttg ccagaacccg gggcgggctt gacttttcct gaagggcaag
      301 ccatatggca ctcgtcctga tcgtttacga cgggacgagg cgcaggcttt gcagccgcgc
      361 ctgccttctc tcccccaccg gcctttccgc cccttgcgag cggctgtgcc ctgcaggcgg
      421 acgagagcgc tccccaccat ggccggcctg ccgttaacag cgctgccggc ctgttttcac
      481 cctcctgcca cgggcccatc atggccgcgg catgatttca tgtttcgagt ttgacgacac
      541 cttttatgca tcattccaag cctcttcaca ccacccagcc ggcgcccctg ccctgcccgc
      601 cagcgccgct tggcgcgcag ccgggcctgc tttcgcgcgc gcaattgcgg cgccttgtcg
      661 ccgatatggt ggattgaccc aaagctcagc cggcggcggg caccagcccg cgccgcgcac
      721 ccatggcatg ggcggccatg gccagccccg cccccacgcc cagagcgccg gccaccacaa
      781 aggccggcgc atagctgccc caatcctggc gcagcaggcc gccggcaaaa gcggccaccg
      841 ccgcgccgat ctggtggccc accaccaccc agccaaacac gatgggcgcc tcacgcgcgc
      901 caaagcggcg caccgccatg gccacggtgg gcggcacggt ggcaatccag tccagcccgt
      961 agaacaccgc aaaaaccgcc agcggcagcg gtgaaaaggg cacaaacggc agcgccatca
     1021 gcgacaggcc gcgcaaggcg taataggcca ccagcaggcg ggccggatca taccggtcgg
     1081 tcagccagcc agaagcggtg gtgcccacca gatcgaacag gcccatggtg gaaagcagcc
     1141 ccgccgctgc caccggcgca atgccatgat cgccgcaata ggcaatgaga tgcgtgccca
     1201 cgaggccgtt cgtcgtcagc ccgcagatga agaacgtgcc gaacaacagc cagaacagcg
     1261 gcacccgcac cgcccgcgcc agcaccctca gcgccagccc caccccggcc gccgcgcggg
     1321 gcggcaaggg cgcatcatcc ggctcgccaa agcggcgcac gccggcatcg gcaggatgtt
     1381 cgggcacaaa ggccagcacc agcggcacca ccgccaggca agccagcccc accgcctgcg
     1441 ccaccggctg ccagcgcgcc ggccccgaca gccaggccag caccggcagg aacaccagcg
     1501 atccggtggc cgtgctggcg gaaagcagcc ccatcatcag cccctggcgc tgcgcaaacc
     1561 agcgccctac caccgctgct gccagcacgc tggccaccgc cccgctgcca aggcccgaga
     1621 tcacgcccca gctcagcagg taatgccagc tgcgtgtcat gcccaggctg gccagcgtgg
     1681 ccagcgccat cagcatcagc cccagcagca tcgtgcgacg aatgccgaag gagagcatca
     1741 gcgccgcggc aaacggcccc accagcccat agaggaaaat gccgatggcc gaggtgagcg
     1801 acacctctgc ccgcgaccag ccgaaatgga gctgcaaggg caccatcatc acgctgggcg
     1861 ccgagcgcac gcccgctgcc accagcagcg ccaggaaggt gaccgccgcc acgccgatcg
     1921 ccggatggcg ccctgcccat ctggcgccca tggtcaggcg tcggccggct cggcccgcag
     1981 gtccaccgcc acccggttgc ggccctcgcg cttggcctga taaaggctgg catcggccgc
     2041 ggtgatcagc gtggagccat cgcgctccat atgggcctcc cagctggcga tgccgaagga
     2101 catggtgatc gcccccagcg tgcgcccgcc ctggtgcacg gtcagctcgc tgatcgcctg
     2161 gcgcaccact tccacgcgcc ttgccagcac atcggcggtg gagcccggcg cgatgatggt
     2221 gaattcctcg ccgccaaagc ggcagatcac atcgccatcg cggaagcggc tgcgcatttc
     2281 ggcggccacc gcctgcagca cggcatcgcc ggcatcatgg ccgaactcgt cgttgaagcg
     2341 cttgaaatgg tcgacatcgc acatgaccag gctgagcgga ccgcccgagc gcagcacccg
     2401 cgcgatctcc agcgccagcg tctcttccat gtagcggcgg ttgaacaggc cggtcagcgg
     2461 atcgcggatg gtctgctcgc gcaggccccg ctgcaggcgg tggttcacca gcgccgaggc
     2521 gatgttctcg gtcagcaccg tcaggcggaa gcggttttcc gcctccacca cgccgcgcag
     2581 atagagcacg ccgatcacct cgccgccggc cagcagcggc tcgcaatgat aatggtcggt
     2641 cacatccacc gcatgggcgc agacgatatc ggagcccggc tcgatgacga aatggctctg
     2701 cccgcgccgc agcgcccagc actgatccgg cggaaagccc accgcttcca cttccagccc
     2761 gccccatgcc gcaatcggca ccagcaggtt gcgcgaattg ttgtgggcat acagcacgcc
     2821 gggaatatcg ggcagcacgc gcggcacgaa aacgcggatg atcgcggcca gttcctcgtc
     2881 ggtgcgggcg gcctgcatgc ggtgggccat gccgcccagc agctcgatca cctcgccgcg
     2941 ctcgcgcagc acttcggcat tgcggctcag ttccagattg gccagccgca gccgctcctc
     3001 gctgtccacc tgatcggcca cggccgcggc cagctcgccg gccatggcgt tataacccac
     3061 cgccagccgc tcgaactcgc tggagcgcat ggcggtgtcg atgcgggcgc tgcgatcgcc
     3121 ccggcccagc tgccccatca ccgccagcat cgcttccacc ggctggcgga tgcggccgct
     3181 gagcacaatg cacgccccca gcaccagcag catggtgacc ggcgtgccca gcagcaccag
     3241 ccagccgatc gcatccagcc gataggtggc ggtggccatg cgggtgccgg ccagggcccg
     3301 ctcctcctcc accagatcgc ccacgcgggc atcgaccagc accatcagat atcgcccgcg
     3361 cccgctggcg atattggcct gggcgccggc aaaatcgccc ttgcgggcca gccccgccga
     3421 ttgctgcagg ctggcggcgc gatcatcgat cagcgcgcgg atctgccggg cgcggctgtt
     3481 ttgcgagggg ttatcctgtg tcagctgaac gatgccggcg gcgccctggc gggccgcggc
     3541 gatctcggcg tcggccgttt cggcaaacac cggatcatgg gtgagcacaa agccgcgcag
     3601 gcccgattcg gccgcgcgca gatgatcgag cgagcggttc gccgttttca gcacttcgga
     3661 ggaatgggcc acccagccca ggctctcgcg catctgcgcc gactggtcga gcagcaggcc
     3721 ggcaaacagc accagcacgc cgcataacac agcgcacagc acaaagatcc gacgggacaa
     3781 tgatccggcc aaggcaaatc cccaaaatcg cctgccgggg ctatgcccaa agcgctggcg
     3841 atgcaatgga aaaggaaatg ccccgcgcga tgccccggcc ctgcgccaac ggcgcccccg
     3901 gggccgccta ccggccgcaa gcggggcgga cggcgggttt tccgggcatt gtcatcaatc
     3961 tgtgcctttg cgtcaatttt atgtcatgtg cagcgggtac aggcggggtt ctgtcatgtc
     4021 cctgtcgcag tcggagcgcg cgatgaacgc cacatcccgg gaaacgcccc atttcgatgt
     4081 ggcccgcgcc gcgcaggtgg ccgaggccta tgccggctcg atcgatccga tcttctcgct
     4141 gggcacccag ccgctggatg gccccggcgc cgatgtcggg ctggatgcct atcatgccgg
     4201 cgccatgctg atcggccggg cgcacatgca cggcgcccgc ttcgattatg cgcgcgaccg
     4261 ggccaagatc gcccgcaccg ggctcgacat gatcctcgtg cagatcatca ccgccggcag
     4321 cgatgtgcgc ttccatggcg atgaggtgca ggtcacccgc ccgggcgaca tctgcatcga
     4381 cgatctcacc cgccccttcg ccacccgcgc cacggattgc gagaacatca acatcatcct
     4441 gccgcgcgcc gcgctggggc tggatgcgct gcagcttgac cgcatccacg gcctcagctt
     4501 tgccgccgac agcctggccg cccggctggt gagcgcgcag gccaacgccc tgctcgaccg
     4561 gttcgacagc tgctcgcccg gggaagcggc ggccgccgcc cgcgccaccg gcctgatgat
     4621 cggcggcctg gccgccccgc tggccaacca gcgcgaggcc gaagaagcca tctcggtggc
     4681 cgtgctgcac cgggtgcagc gctatatcca ggcccatctc gcccggccag acctgacccc
     4741 cgatggcatc gcgcgggcgg tcggcctctc gcgcgccagc ctctatcgca tcttctcgcc
     4801 catcggcggc gtgcgcgaat atatccgcca gcagcggctg ggccgcgtct ttgccgatct
     4861 gcgcaacccc cggctgcgcg cccgctcgat tgccgaaatg gcctatggct ggggctttgc
     4921 cgactggtcg tcgttctcac gcgcattcaa ggcgcaattc gccgtgacgc caagcgaggt
     4981 gcgcggcctg gccggcgtgc tggccccggc tgacagcgcg ggcatcgtgc tgccgcaatg
     5041 gctgcgggag atggaagcgg gcgagatcgg cccggccatg gcctgaggca tgatggctta
     5101 aggcgccgtc agcccgcgct gccctggggc gcctgctggc cctgcaattg cagcacatag
     5161 cggcgcagca gccacatgca gcgttcgaag atggtggtgg cgatgaaggc gcgctgctgg
     5221 gcatcgggcg gcagggcgct gccctgcatg cggcggcgga tgccatccat cagctctgac
     5281 cggtcatgcg tcagcgcgcg cagcaattcc aggtcctcgg catcggcgct gctgcccaca
     5341 tcggccagcg tttccagcac catgtgcagg ctttccacca ggctgtgcat cagcgagagc
     5401 acgccgcgat catccgcccc gcccacgctt tcatgcagct cgaacagcga gttctgcaag
     5461 gcggcgatca gctcgttgcg atcgcgcagg cgcatcacct gctcgcgcac ctcgcggtcg
     5521 tcatagctgt cggccagcgc cgccaggaac atgttgcatt cccgcgtgac agagccatcc
     5581 gcctccagcc gcgcggccac cggcagcagc cccgcgccgc cctcctcgcg cagggccgcc
     5641 agataatcgg gcaggctgcc cagcaggcgc gcctgctccc tttcggccac cagaatggcc
     5701 gtgcccgcat cggccagcgc atcttccgac agataatgcg ggcggcccag cacttcctcg
     5761 accgacggcg gcgaaagcgc gtcgatccgc cgggcaatcg gcccttccac cagcgccatg
     5821 caggccgtgg aggccagctg cagcgccaga tagacccccg ccagctcgcc cgagcgcgac
     5881 agatggagga agccgaaggc aggcgcgatg atccgcccgc ccgccagcca atcggccagg
     5941 aacagcccca gcaggcccgc cacccccacc gccttcagcg ccagttgata gagcaccagc
     6001 tggcgcgcgc tgcccttcag cttgcccgcc agcggcagcg ccgacaggcc cgagcccagc
     6061 cccgccccca tcaccaccat cgcccccgac aggaacggca gcagccccgc gcccgccatc
     6121 gccatcgcca gcaccgccac cgtggtggac gactggatga ccatcgccac cgccgccccg
     6181 acgaggaagc tgagcaccca gaagcgcccg gaataggcca tcgccacatg cagcaggctg
     6241 ctgtcgcgca ggccatgggc ggcggtcttg atgaaatcga cgccgaggaa caacagcccc
     6301 acccccagca acgcattgac gacatgacgg tgccggctcg actgatcgag ccccaggtaa
     6361 taggccgccc ccgtcatgcc cagcagggtg aggatcgcca gatgcatgtt gagcgcggcg
     6421 atcagcacca gcatcgatgt gcccagattg gcccagctga ccaccggaaa agcccgccgc
     6481 ttctcgatcg tgccggccga caccagcgcc accagcacat aggtgacggc attgacgctt
     6541 tgcatgatcg cgcccgccag caggccaaac accgccgtgg aaaccggccc tgccaccgcc
     6601 cgcgtgatca gctggcgcag cttgcgcccg gccatctgct tgagattgct gccgatcagg
     6661 cggatgccga tgaaaaacag gccaagaccg gcaaacaggc tggcgaaaat atccatggcg
     6721 ggccaatgct cgaaagtcag ggactgaaaa ggccggccag ccacaggcca gccgcttgcc
     6781 gcgcctccta aacaggtttg gcgcccgctt tccacatcga aatgaaaagc ctgcgcaaag
     6841 gtgcccgccc gcgcatggca gggcgtatca ttcctgttgc attacgtctc attgcggcaa
     6901 agcttcaaaa tcaatagtct ggaatataaa agaatttcag aagtgtggaa attcatgccc
     6961 gctctgccgg tttgacgccg gttggcccgg cgcattaacg aggcgccact tcctctcaga
     7021 caatcgagcg agccatgacc tccttgcctg tgcccggcag cgcccggccc gatgccgccc
     7081 ataagggcaa ttccgcaggg gccctggccc gccggcccgc atctgcccgg cccgcatccg
     7141 ccaagctggc cagcctggcc ctgctggcca gcgccggggc gcctgtggcc ctgcacgcgc
     7201 aggagatcgc cagcccggcc cccgccgatg ccccgctgcc agcagatgct tcgctgccag
     7261 cagattcagg gcccgccgcc agccccggcg aggcccatgg gctgaaggcg ctgcgcctct
     7321 ccggctttgc cacgctgggc ttcacctata acgacaacag ccatgccggg gtgatcacct
     7381 cctatgccca ggcgcacccg gccaacagcg gcttttccgc cgatctcgat tcggtcgttg
     7441 gcgtgcaggc ggtgtggacc ccgctgaaag gcacctcgat caccgtgcag ctggtggccc
     7501 gccccgacaa tggcatgaag ccggccgtgc gcatggccta tctgcgccac gagctcacgc
     7561 cttcgctggc ggtgcgcggc gggcgcatcc gcagcccgct ctatttcgat tccgacgtgt
     7621 cggaagtggg ctatgcctat ctcaccgccc gcccgccgat cccgctctac accatcgtca
     7681 acagtgtcga cacgctggat ggcggcgatg tgcaatggcg ccacagctgg catgatgtgg
     7741 ccgtgctgct gcaggcctat tacggcaaca gccagtatcg ccaccatttc tacaacgacg
     7801 gctttgccgc ccatgccaag ctcaccggca tcaagggggg cgctgtcagc ctgatcctgc
     7861 cgtggatcac cctgcgggcc tcgcgcacct ggatttccag ctacaccatg cgcggcggcg
     7921 cgatcgacac gctggatgcc gggctggaaa cgctggccac gcagatgaac gcggccgccg
     7981 ccaaccccta tctgccggcc ggctatggcg cggcgctggg ggcgcaggcc tcggccatct
     8041 cggcgctgat gtatccgttc gacaaccatc ccatctatac cagcgtcggc ttcgatgcct
     8101 cgttcaacaa gctgagggtg ctgggcgaat gggccgagct ggattcgcgc tcgcgcatgg
     8161 tggggcgcta tcagggctat caggtcacgg tgggctatgc actgggcaat gtcacgccct
     8221 atgccaccta tgcgcgccag cggcggctgg gcccggcgct ggataccagc gcgctgaacg
     8281 ccaccggcct tagcgaacag ctggatgccg ggctggcgca gatgcagcag gcgctggcac
     8341 tggcgggcag ctatgccaac ctgtcgggca gttcgctcag ccttggcgcg cgctgggatg
     8401 tgcgcgaaca tgtggcgatc aaggcgcaat acgaccggct ggaaacgccg gccagcacca
     8461 ttccgggcac ctttgccgtc agcgcgctgc ccattcgcaa ccgggcgaac ctggccaccg
     8521 tcaacctcaa tctcgtcttc taggagcgcc ttgctgtgaa gacccccgcc gccctcatgg
     8581 ccgccctgat cgccgcgatg gcgttgcccg gcgccgccca cgcacaagac agcgtggtgg
     8641 tgatcgccaa caaatccgtg ccgctcgacc ggctggacaa ccagcgcgcc acccagatct
     8701 tcctcaagca gatccagacc tggcccgatg gcaagccgat cgcccctgtg gacctgaagg
     8761 aaggcgcccc cttgcgcgcc gaattctacg ccaaggtgac agggcgcagc gccggccagg
     8821 tgcgcgccta ttgggcccgg caggaattca ccggcatggg ctttccgccc cggcaggtgg
     8881 ccagcccggc cgatgtcacc cgctttgtgc aggcaacgcc gggggcggtg ggctatgtca
     8941 accgcaaggc ggccgatggc tcggtcaaga ccttgctgaa cgtcagccgt tgagcactgc
     9001 cgcgcctggc gcagaagcgc ggcagggcgc cggcagtggc ctgttcgcgc ccaccgtgtg
     9061 gctgtcggca aggctgagct tttcgcgcaa atacatggtg atcggcctgc tggtgatgat
     9121 cgccctggca gcgctgagca cgccgctgct gctgcagatc aaccgccaga tcgaaaccgc
     9181 cgcgcgtgaa cgggtggggc tgggccatgt ggcggcgcaa accgatctgc tggcagggct
     9241 ggtgcgccgg cgcagcgcgc tgctgggcaa tgaccgcctg cccgatcccg cagcgctcga
     9301 cagggctgtg cgccaagccg tgcggatcgg ccgggccgac gggctggagg ccgatgccgc
     9361 ccggctggag cagggctggc aggcgagcca gggcgaactg ggcgagccgc aggttcgctt
     9421 tgccgccgcc accggggcga tcaacgccgc cctcgccctc atccgcgacg aagcgcggct
     9481 ctatcggctg aatgtcgatc ccgatctcga tgcggcgctg gaaatgctca gcctgcgcct
     9541 gccgctggtg ctcgatatca tcggcaagca gcgcgatgcg ctggccatcc gccaggccga
     9601 catcacctcc tatgcgctga gcgcgcaagt ggcgctgacc gaatcggccc ccgtgctgcg
     9661 cgccggcgtg aaccagctgg tggccgccgg cgccgaccct gcgctggggc cgcagcttag
     9721 ccgcctgctg gccgatatcg cccgccagca ggacagtgtc gatcgcagcg tgggggccgc
     9781 agccaaccgc gaggaactgc agcagatcgc ccagggcaac atcgccagcg cgcaaggcct
     9841 gctggccaag gccgccagcg cggtggacag ccggctgcgc cagcgcatcg aggccctctc
     9901 gcgtgagcgg gcggtgatcc tcgcgctgat cctgggcgag ctgggcgtgg tgttctacct
     9961 gttcgccggc atctatatct ccaccctgca atcgctgaag cgcctgtcgc atggcacacg
    10021 ggccttctgc agcggccgca tggatgcccg catccagatc gacacccatg acgagctggt
    10081 gctgatcgcc cgcaatttca acacgatggc cagcgaggtg gagcgcctgc tgggggttat
    10141 ccagcagcag aacgaaagcc gccaggcaga gctggaaagc gaagtgcggc tgcgcaccgc
    10201 cgaactggcc gagcgcaacc gcgaattgca gcgcgccggc aagcgcgtgg ccgaagagct
    10261 ggatctggcc cgcagcatgc aaaaggcgat cctgccgcaa accttccccg acgaggccgg
    10321 ctggggcgtg catgccgcca tgcaccccgc gcgcgagctg ggcggcgatt tctacgattg
    10381 cgtgccgctg ccggggggcc gctatggcgt gctggtggcc gatgtggcgg gcaagggcgt
    10441 gggggcggcc ttcttcatgg cggtggcgcg cacggtgctg ctggatctgg cgctgtcggg
    10501 cctgtcgccc gaggcggtgc tggcccgcgc caacgacctg ctgtgcgagc gcaatccggt
    10561 cgagctgttc gtcaccacct gctatgcggt gttcgatccg ggcgatggca gcctggtcta
    10621 tgccagcgcc ggccatcatc cgccgctggt gcgccgtgcc gatggccggg cccagacgct
    10681 ggcctcggcc aatgaggtgg cgcttggcgt gatgccgcag atggcctatt cccttgcccg
    10741 cgcgcaactg gcgccagggg aggccgtgct gctctacacc gatggcatca ccgaggcttt
    10801 cgatgcggcc ggcgatccct atggcgatgc gcgcctggcc gcctggttcg agcgctgcgc
    10861 gcccgatgcg ccggcggccg atctggtggc ggggctggtg gccgatgtgg cgcgctttgt
    10921 cggcggggag gaagcctccg acgatctgac atgtctggtt ctgtgccgca aatcaggaga
    10981 gctgcccatg caggatcccc ccaaacccgc ctcggtgccg gcgctgccgg tggagatcgg
    11041 ggacaagcgg caattgctgc accggctgat cgattcccgc gtggaagaga ttgccggcct
    11101 tgccgaggcg atcgaggaag cgctgccgga tcgggccgat ctcgcctttg tcgccaacct
    11161 gtgcctggaa gagctgatca ccaacaccat cctgcatggt tttgccggcc agccgggcca
    11221 tgccatagaa gtgttcctca accgttcgga ggaatggctg gagatcatcc tgaaggatga
    11281 tgcccccccc ttcgatccct ttgtcgaggc gcccgcgccc gatatcgagg ccgatctcga
    11341 cgggcgggcg gtgggcggcc ttggcgtcta tatcgtcaag accatgatgg atgcagctca
    11401 tgcctattac gatggcggcg gcaatttgat cgtgctgctc aaggcgctac aacagcccga
    11461 atcgcccacc caatagcccc ttcacctgca caccgggaat tgccatgagc ttcgaggccc
    11521 aactgaacgt cgaacatgcc atcaactggc tcagcctcag tggccggctc gacagtgcca
    11581 cggccaatga tctggagcag gtgctgctgc cgctgttcgc cgcgcccgcg cccaccgtgc
    11641 tggtggattt tgccgggctg ggctatattt ccagcgccgg cctcagggtg gtgctgatgg
    11701 cggccaagcg ggccaagcag gccggtgggc ggctgatgct gtgcggcctt tccagcgccg
    11761 tgcgcgaagt gttcgagatc agcggcttcc tcaagatcct cgaagtgagc gacagccgcg
    11821 ccgaggccga gcgcctgctg ctggcctgag gcatcagccc ccgcgccagc gcggaaaggc
    11881 cagttccagc gcaaagcccc gatcggccgg cagcagggtg caggccgccc catgcatatc
    11941 ggcaatcgct gccaccagcg acaggcccag cccggtgccc ggcagcccct tgctggcagc
    12001 gcagcgatag aagcggcgga agatccgcgc atgatcggcc ggatccaccc ccggcccgtc
    12061 atcgctgacc gttgcgcaca ccctgtcgcc ccgcgccgcc agcgcgatgt ggatggccgt
    12121 gcccacaggc gtgtggacga tggcattctc gaccaggttg atgaacagct gggtgagcaa
    12181 ttcggcatcg ccttccaccc acaggcccgc cgccaggctg gcgcccagcc ggtggccggc
    12241 atcctcggcc accggcgcat aggtctcgac aatgcgcgcc agttcctccg acaggtccac
    12301 cggctgaaac cggccgcgcc ccatgccgcc ctcgatcatg ccgatccgca gcagggcctg
    12361 gaaggtggaa agcaggccct ccacctcgcc gatggccagc tccacccccg catcggcatc
    12421 gagccgcgcg ttcaggcttt ccagccgctg gtgcaggcga gtgagcggcg tgcgcagatc
    12481 atgggcaatc gccgtggaaa cctggcgcaa cccctccatc gccgcatcct ggcgctccag
    12541 catgtggttg aggttgcggg tcagcgtgtc gaattcgggc gccaggccaa tggcgggcag
    12601 gcgctcggcg ccattgccgg caacgatccg ctccacctgc cggttcaccc catccagccg
    12661 gcgcagaaac agccgcccca cgccatagcc gccaaacagc gcgcacaggc tgatcgccac
    12721 cgcgcccagc agcgtgtagc gcgcgaggcg atggcgcagg ttctcgatat cgaagaaatc
    12781 ggtggccacc accagcagct ggccatcgcc gatgcgcatg ccgaactggc gcatggccac
    12841 agggccgctg tcgggctcgg ccagggtgat ggtggccgcg cccgggcggc ccagcccggc
    12901 cggaatgtcg ccaaacaggc ggcggccctg agcatcctgc agcaggtaat ggaagggctg
    12961 ctcacgcgtg ctggcgctgt ggcgttgcag cgcgttgagc agcccttccc tgccggtctg
    13021 cgcatattcg ccggccagca cccgcgcctc ggcgtgcagc atggcctctg tcgcctcctg
    13081 gccataggcg gccacctgcc gatccatcat ccacagcagg cccagcgagc tgagcgcgaa
    13141 caccagcgcc agcgccagcg caaagcggaa cgccccgctg cgcagggccg acaacggtgt
    13201 ggcttcaatg ctcatgcctt actgctcatg gaaacgataa cccgcgccgc gcagtgtctg
    13261 gatcggatcg ggccggccgg tttcgcacaa tttggcgcgc aggcggctca tgtggctttc
    13321 gatgatattg gtgcgcgtgt cgaaatgata ggaccagaca ttctccagca gcatgctacg
    13381 ggtgaccacc tggcctgcat gcagcatcag atattccagc agcttgtatt cctgcgcctg
    13441 cagccgcaag gcttggccgc cgcgcgttac gctgcgcttc agccggtcga tctcgatatc
    13501 gccgatgcac agggccgaag gcacatcggc cagcggcacg cggcgcccca gcgcatgcac
    13561 ccgcgccaga aactcgcgcg cggcaaaggg cttggccaga taatcatcgc cgccggcatc
    13621 cagcccatcc accctgtcgt ccaccccggt catcgcggtc aggaacaggg cgggcgcgcg
    13681 cacgcccatc gcccgcaagg ccttgagcgc ggccagccca tcgatccctt cgaccatgcg
    13741 atcgaggatc agcacgccat aatcctgcgt ggaggcgaga tagatcgcat cctgcccggc
    13801 gcggcaggtg tcaaccgtgt ggccggcctc gcccagcacc cgcaccagat gctccgagag
    13861 gacggcatca tcttccagca aaagaatttt catcgctgaa ccgcctctct gtcgccaacc
    13921 tgcggatcgg ccgcccggcc gccggccaaa atatgccgat aggccggggc aaggcaaaat
    13981 gccaagttgg caaattgtta tgtttgccca atcctgcggt catgccggcc cggctagagg
    14041 caggcgcatg cctgtgttca tgccttgcct gcccgcccgc ctgcccgccc cccgcttgcc
    14101 tgttccctct ggcgccccgg ctgcccggaa agggcggtgg ctggcggcgg ccggctgcgc
    14161 gctgctggcg ggctgtgccc attatgcgcc actgccgctg cccgcccgcc ccgccctggc
    14221 gcccggcgtg gcggcccttg cgcaaccgcc ggcaagcgaa aggccgcttg gcatcgccga
    14281 ggtggccgcg ctggcgctgg ccaacaaccc cgatctcaag gcggtgcgcc tgcgcgatgc
    14341 ggtggcccgc ggccagaccc ggcaggccgg cctgctgccc aacccgcaag tgacaggcgc
    14401 cttcctgccg ctgctctccg gggtgggcac ggtgtcggcg tggaatgccg gcctcgccca
    14461 gaacctgcgc gccatcgtca cctatcgcgc ccatctgcgc gccgcgcagg acagccagca
    14521 gcaggtggcg gccgatatcg tctggcagga atggcaggtg gcggggcagg cccggcagct
    14581 ggcggcagac atcatcatgg gcgagcaggc ccgcccggcc tatgccgaaa cgctggccct
    14641 gctggcagac cgcaacgccc ggctggaaca ggcgatggcg cagggccagg ccacgctggc
    14701 gcagctggcg cccgaccgcg ccgccctgca ggccgcccgc gccgcgctcg acgcgcagga
    14761 ccagcgccag ctggccctgc gccagcagct gaacgccctg ctgggcctgc gccccgatgc
    14821 cccgctgccg ctggtggcag agccggacct gccccccttc gcgcctgccg ccctgcgcgc
    14881 ggggctcgcc agcctgcccg atcgccggcc cgatctcatc gccctgcgct gcggctatgc
    14941 cgcggccgac gaaggcgtgc gcgaggccat ccgcgcgcaa tttcccgacc tcgtgctggg
    15001 cggcggcatc gccagcgaca attccaaagt catcagcggc gggcccaatg ccgcgctggg
    15061 cctgccggtg ttcgatcgca accagggcca
//
LOCUS       DASRYE010000215         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_118962,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000215 DASRYE010000000
VERSION     DASRYE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118962"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1998
                     /locus_tag="VFF98_10905"
     CDS             148..1998
                     /locus_tag="VFF98_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HZU64185.1"
                     /translation="MKRLLFCLALALPLSATAGNLPPRAGAQPSAAHAGAAAVLGAHR
                     AEAEAPVDIGLIALNDFHGHLLPPHAAMMAPDGRGGAVAVPAGGAAWLASAVDAARAR
                     HPHALTLSAGDILSASPSESALFLDEPAIGVMNRIGLDYNAVGNHEFDRGRAELLRIA
                     HGGCEQTTLRKPCALEPYAGARFPFLAANTLTEDGTPLFPATALRSFGKGARRVRVGI
                     IGLTLRGTGALSSPEGHKGLRWADEADTANALVPQLKAEGADVVVVLIHQGGRTDSAQ
                     PDPNGCSGFHGGLKEILDRLDPRVDLVISGHTHWAYVCHYPVAGGAHSLLVTSAGLWG
                     EAITEIDLKVDPARHLLLSAEARNIIVQSDGYGGPRGAANTPLYPRFAPRADIAAYVQ
                     RYADAAQKLAGQQVGWLAGPAEKTEGALGSTGGPLGRLIADAQLAATRPAGAEISFMN
                     PFGVRASLLPAGDRAVNWGQLFAVQPFGNDLVTLTLTGAEIRALLEQGFGASGPEQAL
                     TPSAGFAYGYDRTRPPGSRIVWATLDGRPLEAARPYRVTVSSFLADGGDSFSLFTQGR
                     NRTIGANDLAALESWLQARPPRALPTDPRDTDLAPATRGNPTPSPPGHHY"
     gene            complement(2019..>2709)
                     /locus_tag="VFF98_10910"
     CDS             complement(2019..>2709)
                     /locus_tag="VFF98_10910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZU64186.1"
                     /translation="VVPGAHWHDDLSTLPQDRPLLVLANEFLDALPVRQMVQTPHGWR
                     ERLVAHDGARFLPVAGRLPMDAAVPEGLRAAAPGAIVEACPAAAAITDELAHRLAAQG
                     GAGLIVDYGYAQAEALSTLQALKAHARADPFADPGEADLTCLVDFATTAAVARRAGAR
                     VRGPQGQGAFLQALGMGARAEALARATPARTQEVARAFHRLVDAAEMGTLFQVLALES
                     PAWPEGAGFPA"
BASE COUNT          382 a         1038 c          912 g          377 t
ORIGIN      
        1 ccaccgcgag ggccgccgaa cccgcacctg gatcctcgat ccggccgaga tcgcccagct
       61 gaccgcccgg ctggaagagg aaggctgacg gccggccggg cgcctcctgt cttgccccgg
      121 caacaatcct gcgctctctt cgcggccatg aaacgcctgc ttttctgcct tgccctcgcc
      181 ctgccgctgt cggccacggc cggcaatctg ccgccccgtg ccggcgccca gcccagcgct
      241 gcccatgcgg gcgccgccgc cgtgctgggc gcgcaccggg cagaagctga ggcgcccgtc
      301 gatatcggcc ttatcgccct caacgatttt cacggccacc tgctgccgcc ccatgccgcg
      361 atgatggcgc ccgatggcag gggcggcgcg gtggcggtgc cggcaggggg cgcggcctgg
      421 ctggccagcg cggtggatgc cgcccgcgcg cgccatcccc atgccctcac cctgtcggcc
      481 ggcgatatcc tctcggccag cccgtcggaa tcggcgctgt tcctcgatga accggcgatc
      541 ggcgtgatga accgcatcgg gctggattat aacgctgtcg gcaaccacga gttcgatcgc
      601 ggccgggccg agctgctgcg catcgcccat ggcggctgcg agcagaccac cttgcgcaag
      661 ccctgcgcgc tggaacccta tgccggcgcc cgcttcccct tcctcgccgc caacacgctg
      721 acggaggatg gcacgccgct gttccccgcc accgccctcc gcagctttgg caagggcgcg
      781 cggcgcgtgc gggtggggat catcggcctc accctgcgcg gcaccggcgc gctgtcctcg
      841 cccgaggggc acaagggcct gcgctgggcc gacgaggccg acaccgccaa tgcgctggtg
      901 ccgcagctga aggccgaggg cgccgatgtg gtggtggtgc tgatccacca gggcgggcgg
      961 acagacagcg cccagcccga tcccaatggc tgcagcggct ttcatggcgg actgaaggag
     1021 atcctcgacc ggctcgatcc gcgcgtcgat ctggttatct ccggccacac gcattgggcc
     1081 tatgtctgcc attatccggt ggccgggggc gcgcacagcc tgctggtcac cagcgcgggc
     1141 ctgtggggcg aggcgatcac cgagatcgac ctcaaggtgg atccggcccg ccacctgttg
     1201 ctttcggccg aggcgcgcaa catcatcgtc cagtcagacg gctatggggg cccgcggggt
     1261 gcggccaaca cgccgctcta tccgcgcttt gccccccgcg ccgatatcgc cgcctatgtg
     1321 cagcgctatg ccgatgcggc gcagaagctg gcgggccagc aggtgggctg gctggcgggc
     1381 ccggcggaaa agaccgaggg cgcgcttggc agcaccggcg ggccgctggg ccggctgatc
     1441 gccgatgccc agctggccgc cacccggcct gccggcgccg agatctcttt catgaacccc
     1501 tttggcgtgc gcgcctcgct gctgccggcg ggcgataggg cagtcaactg ggggcagctg
     1561 tttgccgtgc agcccttcgg caatgatctg gtcaccctca cgctcaccgg cgccgagatc
     1621 cgcgccctgc tcgaacaggg ctttggggcc agtgggccgg aacaggcgct caccccctcg
     1681 gccggctttg cctatggcta tgatcgcacc cggccgccgg gcagccgcat cgtctgggcc
     1741 acgctggatg ggcgcccgct ggaggcagcg cggccctatc gcgtcaccgt cagcagcttc
     1801 ctggccgatg gcggggacag tttcagcctg ttcacccaag ggcgcaaccg caccatcggc
     1861 gccaatgatc tggccgcgct ggaaagctgg ctgcaggccc ggcccccccg cgccctgccc
     1921 accgacccgc gcgacacaga cctggccccc gccacgcgcg gcaaccccac gcccagcccg
     1981 cccggccatc actactgagg ccaccactac tgaggcggtt acgccgggaa gccggcgccc
     2041 tctggccaag ccggcgattc cagcgccagc acctggaaga gcgtgcccat ttccgctgca
     2101 tccaccagcc ggtggaaggc gcgggccacc tcttgcgtgc gggccggcgt ggcgcgggcc
     2161 agcgcctcgg cccgggcgcc catgcccagc gcctgcagga aagccccctg cccttgcggc
     2221 ccgcgcaccc gcgcgccagc ccggcgggcc accgccgccg tcgtggcgaa atcgaccagg
     2281 caggtcaggt ccgcctcgcc cggatcggcg aagggatccg ccctcgcatg cgccttcagc
     2341 gcctgcaggg tggaaagcgc ctctgcctgc gcatagccat aatcgacgat cagccccgcc
     2401 ccgccctgcg cggccagccg gtgggccagc tcgtcggtga tggcggcagc ggcggggcaa
     2461 gcctccacaa tggcgcccgg cgctgccgcg cgcagcccct cgggcacggc ggcatccatc
     2521 ggcaatcgcc ccgccaccgg caggaagcgc gcgccatcat gggccaccag tcgctcgcgc
     2581 cagccatggg gggtctgcac catctggcgc accggcagcg catcgagaaa ttcattggcc
     2641 agcaccagca gcggccgatc ctgcggcagg gtggaaagat catcatgcca atgcgcgccc
     2701 ggcaccaca
//
LOCUS       DASRYE010000216        21500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_12830,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000216 DASRYE010000000
VERSION     DASRYE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12830"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5269)
                     /locus_tag="VFF98_10915"
     CDS             complement(<1..5269)
                     /locus_tag="VFF98_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HZU64187.1"
                     /translation="MATTMIPQSDADPASIFQSNLPIADKLDRARMELLDLSARNRLL
                     NLPRKNARSVEIVDEKSAEIFRLLVREGKVFTFLAGKGARGGDEGETDDADEIMDLAL
                     PEDDVADERGVFSRHSDTRLQTRLTPKGLQKRLLELYFDARTLEEEQGVNILYLTLGM
                     LRWVDPGNAANIRHAPLLLIPVSLERGNAGEKFKLRARPEEFASNLSLEAWLDRVHGI
                     RLPAFEASDGFDPMTYMDEVAEAVAAKTGWAVLHDDMALGFFSFAKFLMYRDLDPANW
                     PDHARISDRPLVRGLLADGFQGAGGMIPEDAKIDPFIPPADMLHILDSDSSQALAVHE
                     VRRGRDMVIQGPPGTGKSQTIANIVASAVADGKTVLFVAEKMAALEVVKRRLDATGVG
                     DACLELHSNKANKRAVLDELRRTWELGAPKGGDPGSLHARLTEARDQLNDHAARMHSL
                     HAASGLTPYQVIGQLARLRLAGEQPNDIALEAPQTWSADGFNQRHNILSELVERVAVI
                     GQPADHVWRGVRLASASPMEVERFVSRFAALGQRLDRFDADQAALAARLEQEAVARLR
                     DVGVLTGLARRVGTAPGLSSRALGSPVWEEQAQEIAALLETGRRYAALREALAPICPD
                     AAWAMDFVGARDRLGHLPTGFSLEAFARLSVLVEHIPRLRAEADALSQAMGRDAPATL
                     ADIRRLLRVGDRVVVAPEASADAFAADLWDNGVERAADIAGAVQRLESARTAIGDRLL
                     DAAWGMDLGAARATLASHGTGFFKVLSGDWRHANRLVRTVLTNPDQGLDETLGQLDAL
                     AEGQAARRAIEAEEAFARSAFGGDWRGDRSASASLLALIEWVRSLKGLGAEPRIVAAN
                     RPDRDAITLRLSRTQALLTELSPVIDALWSDLPEQGVMFGDALSAERADMAALLAAVS
                     GFHDAGQASAAAMATVPADLSLRLRTLDDLAEGQRCSASLMRDDDLGQAAFDTMWQGG
                     TSDWGELRVMADWIESHRDIRLLASRIAHRQALVPSADALERDGDALVADIGGLLGEL
                     SCETYVVLGFSSVAAAPCAHLRQRLAEWAAGGEDLSRWVAYRDRADRARALGCGDVVA
                     RLEGGRLAPGAVIPAFEMAYFEAIHQDQLREHPELGRFDGTLHGRLAREFADLDRQRI
                     VAASLEVVRAHHQRIPPRNGGPPVGPLGTLKTEMERKRGHMPIRRLMERAGPAVQALK
                     PVFMMSPLSVAQFLAPGAFEFDLLVMDEASQIQPVDALGAVARARQVVVVGDPKQLPP
                     TAFFSKVTGNGADGDDDDGGAQVADIESILGLFTARGLPERMLRWHYRSRHQSLIAVS
                     NRQFYESKLFIVPSPYTAEAGMGLRFHHITEGLFDSGSTKTNQVEAKIVARAIMAHAC
                     DHPELSLGVAAFSVSQRRAILDHLELLRRSATPEVEAFFQAHYAEPFFVKNLENVQGD
                     ERDVIFISVGYGPTTPGGRVPMRFGPLGMEGGERRLNVLISRAKQRCEVFSSMTDEDI
                     DPDFAQSRKGVFAFRLFLHFARTGRMTMAESTGRDHDSVFEEQVAKALQARGYHVHRQ
                     VGLAGFFIDLAVSDPERPGRYLLGIECDGAAYHDARSARDRDRLRQSVLESHGWTIHR
                     VWSTDWFHRPNEQLEQIIARIEEAKAEHAGEPHERASRAVPVDIFSIERDDVTEMGLV
                     RVDGEEGDPPQNLYVEAVLNRPTHRTDDLHETPTGILTALLVEAVSIEGPVHIDEVIE
                     RIRSAWGLKRAGGRIQQAIEDAADVALKTG"
     gene            complement(5346..5789)
                     /locus_tag="VFF98_10920"
     CDS             complement(5346..5789)
                     /locus_tag="VFF98_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64188.1"
                     /translation="MRALVDEPLVYRRGFLLLLTAARSNELFGAPASEIVDGLWTLPA
                     DRSKNGEENYVALGPWGYDAHSNALGVGCDASVHFRDPRTGAFGQAWSVGGKAWHLRH
                     KGEQLMAFVVFSDAEAFCVYMVAALRTNTDVLDVIRCLRRRDLTP"
     gene            complement(5946..6176)
                     /locus_tag="VFF98_10925"
     CDS             complement(5946..6176)
                     /locus_tag="VFF98_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64189.1"
                     /translation="MLAVAMAHTIYMEAMRRGDRRKLKPRTLCDKEVIFSRDIEPRLG
                     SVSLYQLSENECWDSVYDKAKASKVRANKMAG"
     gene            complement(6655..6731)
                     /locus_tag="VFF98_10930"
     tRNA            complement(6655..6731)
                     /locus_tag="VFF98_10930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6695..6697),aa:Met,seq:cat)
     gene            complement(6877..7854)
                     /gene="mltG"
                     /locus_tag="VFF98_10935"
     CDS             complement(6877..7854)
                     /gene="mltG"
                     /locus_tag="VFF98_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZU64190.1"
                     /translation="MTRKQRRRLIAIGAALAVGLPALNLAWSWWAPGPLPADTQVTVP
                     DGASLASVAMRLEKGGVVRSAFNFRWHAKLLAMGRKLKAGEFLLPAGASESQVLAIIT
                     GDNALRRFVTVPEGLPSVMVQEKLLSETMLAGPAPLPAEGSILPETYDFARGESRAEV
                     VGRMQAAMTKALAEEWAARAPDLPVKTPAEALTLASIVEKETGKSEERAMVAGLYENR
                     LKKGIMLQADPTIIYPITKGKPLGRRIRESEIHAVNGYNTYAMAGLPDGPITNPGRAS
                     IHAVLHPAKTEVLYMVADGSGGHVFATTLDEHNRNVARWFAIRRARGEL"
     gene            complement(7856..9115)
                     /gene="fabF"
                     /locus_tag="VFF98_10940"
     CDS             complement(7856..9115)
                     /gene="fabF"
                     /locus_tag="VFF98_10940"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZU64191.1"
                     /translation="MRRVVVTGLGLVTPLGADVETAWANILAGKSGAGRITRFDTTGQ
                     KCHIACEVKPADHEYGFDAGKRVDHKIQRQVDPFIVFGIDAAGQALEDAGLVDMPEEM
                     RLRAGCSIGSGIGGLPGIESESLVLAEKGPARVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMIRDGDADIMLAGGAESTICPIGIAGFAQARALSTAY
                     NDQPEKASRPYDKDRDGFVMGEGAGVVCLEEYEHAVARGAKIYAEVVGYGLSGDAYHV
                     TAPHPGGSGAYRSMEMALKKAGMTPADIDYVNAHGTSTPMGDELELGAVRRLFGDAIS
                     TVSMSSTKSAIGHLLGGAGAVETIFCILAIRDQIVPPTLNLDNPSESCEGVDLVPHTA
                     KKRPVRAALNNSFGFGGTNASLVVKAL"
     gene            complement(9339..9575)
                     /locus_tag="VFF98_10945"
     CDS             complement(9339..9575)
                     /locus_tag="VFF98_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU64192.1"
                     /translation="MSDTAERVKKIVVEHLGVEAEKVTEDASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKISTVSDAIKYIDEHKG"
     gene            complement(9744..11552)
                     /gene="aspS"
                     /locus_tag="VFF98_10950"
     CDS             complement(9744..11552)
                     /gene="aspS"
                     /locus_tag="VFF98_10950"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZU64193.1"
                     /translation="MQSPVTDTLAHPYRSHTCGALNQDHVGQNVRLSGWVHRKRDHGG
                     VLFVDLRDHYGITQIVADSDSAALPVLESLRVESVVSIDGVVKARGAGTVNANLATGA
                     IEVFARVATVQSAAAELPMPVAGEQEYPEEIRLKYRFLDLRRETLHANIVKRTKVISA
                     MRRAMEDTGFTEYSTPILTASSPEGARDFLVPSRIHPGSFFALPQAPQQYKQLLMVAG
                     FDRYFQIAPCFRDEDPRADRLPGEFYQLDLEMSFVTQEEIWETLEPVVTGIFTQFGQG
                     AAVTPAGEWPRIPFDEAMLKYGTDKPDLRNPLIISDVSHHFTSSGFGLFEKIVGSGGV
                     VRAIPAPATAEKSRKFFDEMNEWARAEGHAGLGYVTRKGGEFGGPIAKNHGAEGMEKL
                     YAELGLGENDGLFFAAGKSEQAAKLAGAARIRVGELLDLIEKDAYRLCWIVDFPFYEW
                     NEDEKKVDFCHNPFSMPQGGLEALNAGDPLAIKALQYDAVCNGYEIASGSIRNHLPET
                     MVRAFEITGLSREDVEARFGGLYRAFQYGAPPHGGAAFGVDRIVMLLCGAHNLREITL
                     FPMNQRAEDLLMGAPSAATMKQLRELHIRVVEQPKG"
     gene            11779..12975
                     /gene="rnd"
                     /locus_tag="VFF98_10955"
     CDS             11779..12975
                     /gene="rnd"
                     /locus_tag="VFF98_10955"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZU64194.1"
                     /translation="MKIHPLITTTDALADLCGRLARAQFVCVDTEFMRESTYWPELCL
                     IQIADDKEAAAIDPKADGIDLSPLLDLLVDNEEVLKIFHAGGQDVEIIYNLTGKTPHP
                     IFDTQIAMMAISQSEQIGYSNLVESWLGLVIDKGARFTDWSRRPLTERQIEYAIGDVT
                     HLARIFPKILKRLIKTGRGEWLDAEMEKLADPENYRNDASLAWLRIKAAGRNPAMLGR
                     LKALAAWREYEAQDKNIPRGRIARDETLADMASHPPKAQADLAKVRGLSPGWKDNEIG
                     KRLMLALADAQPLAEDELPPRAPRGAPLGKEGSLVADLLKLLLKIRAREIDVAARLLA
                     RSDDLELLAAGQRKGISILEGWRYEVFGHDALDLVEGKLGFAVVHGRLKMTRLDESAD
                     TPEGAA"
     gene            12978..13874
                     /locus_tag="VFF98_10960"
     CDS             12978..13874
                     /locus_tag="VFF98_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HZU64195.1"
                     /translation="MSVYQPTLKQLQYLVALHEHGHFGRAADSCFVSQSTLSAGLRDL
                     ESLLGVTLVERTKRAVHFTPLGNQVVAKAHRLLREAEELSDLIQASSQPLCGEIRMSV
                     IPTIAPFLLPRMLPRLRQERPALKLFLREEPSGAAIESLQHGRADCVLLALPFATGEV
                     EKETIELDALYVAFPKDDPRDPPEEISPDLIDEHRLLLLEDGHCLKDHALAACNRPEL
                     RASATMIGTSLHTLVQMVDNGLGLTMLPEMALDAGILNGTNVVARPLQSPTANREIAL
                     VWRRHSPRTEEFRLLAEELRAG"
     gene            13977..14129
                     /locus_tag="VFF98_10965"
     CDS             13977..14129
                     /locus_tag="VFF98_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64196.1"
                     /translation="MRRITLALAALATTAAFAAPAMAHPHKQPHKAAQHGKAAHGHGQ
                     VKKGHH"
     gene            complement(14191..14784)
                     /gene="pgsA"
                     /locus_tag="VFF98_10970"
     CDS             complement(14191..14784)
                     /gene="pgsA"
                     /locus_tag="VFF98_10970"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZU64197.1"
                     /translation="MLTLPNLLTLSRILAVPFLVWLMWWPDWPLGYGLAFAMYGLMGI
                     TDYFDGYLARAQGTVSKLGVFLDPIADKIMVAAVILVLTAKGVLTGPYVQDLHVIAGL
                     IILLREIAVSGLREFLGGIQVSVPVSKLAKWKTTFQIICLGALILGVAMPWWNVVVWG
                     IEANVPHTVGLITLWGAAGLTLITGWDYLRVGLKHMD"
     gene            14898..16211
                     /locus_tag="VFF98_10975"
     CDS             14898..16211
                     /locus_tag="VFF98_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64198.1"
                     /translation="MTQSTKLLRGRRFLPLFVTQLLGAFNDNLFKNAIVFFVVYSVYD
                     SDKAEAKFSAISSAVFILPFFLLSALSGQLADMRDKARIIRFIKLAEIGIMLVGACGL
                     LLAWRGIGTQDLAIPLMLLALLGMGTHSTFFGPIKYAILPQHLADKEVLAGTGLVEAG
                     TYLAILAGTILAGWIPVQWAAIGVVITALIGFVSSLAVPSAPPLGAIEPLDFHFVRAS
                     IRLVRNTMEDRRVFLAIVAISFFWAVGAILFIEFPPLAKNVLTASKEVASLFLVIFSV
                     GVALGSVSINALLKGRVSARYAPASVLVMAAFIIAFEQVCQAWQPQTDGSLLTVGAFL
                     VHPLAPALLGTLAGIAIAGGMFVVPLYAFLTTFVEKSQTARTIAANNIVNSGAMVLGA
                     SLAVGLSALGVSIVQQVLLSAAMCLISAWLSYRLFRAEDDGPQPA"
     gene            complement(16212..16418)
                     /locus_tag="VFF98_10980"
     CDS             complement(16212..16418)
                     /locus_tag="VFF98_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64199.1"
                     /translation="MRRALVLVDDAARHPFRQGLPLHVRRMSTGRAPALWWPAVQGLR
                     GARALREFLMAYCACFVAVCVFIG"
     gene            complement(16507..16923)
                     /locus_tag="VFF98_10985"
     CDS             complement(16507..16923)
                     /locus_tag="VFF98_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZU64200.1"
                     /translation="MSEINYWLMKSEPNVYSWDDLVAEGEGTWDGVRNHRAANNLRAM
                     KPGDLVFFYHSNIGLEIVGIATISVGGLSDPTDETGKWAAVKLKPVEKLAHPVTLKRI
                     KADPALAEMELVRLSRLSVAAVRPEEWAYILKLSQD"
     gene            17046..18002
                     /locus_tag="VFF98_10990"
     CDS             17046..18002
                     /locus_tag="VFF98_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZU64201.1"
                     /translation="MPHSLAALPVLSLAAPRADFARDIGASFRRFGFALVADHGIDAA
                     LIAEGWRLTEAFFALPEAEKRRWHVPGIGGARGYTPFRTEIAKGASAHDLKEFWHVGR
                     DLADSEYAAGTALAASMPRNLWPDAPAHFADTFRALYAAFDAAGARILAAIALDLGLA
                     EDWFAPAIDQGNSVLRLLHYPPIAGASCGAIRAGAHEDINLITLLLGAEEAGLELLTK
                     QGEWQEVAPPPGALVINIGDMLQRLTNHVLPSTTHRVRNPDAARAAHSRYSMPFFLHL
                     RSDFAFHTLPQCITPENPDRYPVSITADDYLQERLREIGLTK"
     gene            17999..18973
                     /locus_tag="VFF98_10995"
     CDS             17999..18973
                     /locus_tag="VFF98_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZU64202.1"
                     /translation="MTDRPGILITAPLLPQIETALDAAFPLHRLWEAPDRAAFLAEVG
                     PGVRGLATAGYFGRVSEDLLCQLPALEIIASFGVGYDTIDTAAATAYGVVVTNTPGVL
                     DDEVADLTVGLLLATIRQIPQAERFVREGQWLKGAFPFSASLRGRKVGILGLGAIGKA
                     VARRLEGFGVEIAYHGRHRQADVPYAYHASPLELARAVDTLVVLTPGGAATQHLVNAE
                     VIDALGPQGVLVNAARGSVLDQAALVAALREGRLLAAGLDVFAEEPCQPADLLALPQV
                     VCLPHVGSATHKTRAEMGQLLVDNLTSWFAGTGPLTPVAESRALLAPA"
     gene            complement(18959..21031)
                     /gene="recG"
                     /locus_tag="VFF98_11000"
     CDS             complement(18959..21031)
                     /gene="recG"
                     /locus_tag="VFF98_11000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZU64203.1"
                     /translation="MRPDLLNPLFASVESLKGVGAGLARPLDRLGLTRIRDLAYHLPE
                     RFVERRVIATLAEGAPGDQVILPLVVRAHKLSAGRGPFRIIAEDGAGESVALAYFGRN
                     GGWAARQFPLGETRWVAGRLDQFGGAWQIVHPDHVAAEASALAGQQREAVYPLSEGLT
                     QGRLGGLVAQALSRLPELPEWIEPGLLAREGWPAWRAALAEAHSAEAKKARDRLAYDE
                     LFANALALMLVRQQGRRREGLPLRGDGRLRARLALPFALTGAQARAIAEIEGDMAQTA
                     PMLRLLQGDVGSGKTVVALHALLIAVEAGGQGALLAPTEILARQHFETLSRMAAPTGA
                     RVALFTGRDKGKARESILMGLMDGSIDIIVGTHAIFQEGVAYRNLALAVIDEQHRFGV
                     GQRLMLTQKGRRTPHCLAMTATPIPRTLTLAQYGEMDVSRLDELPPGRQAIDTRVVAI
                     ERLDDVLTALARHVAGGGQAYWVCPMVRESETADLAAAEERHALLHDRLGAAVELVHG
                     QLKPEVKDAAMARFAAGEAQVLVATTVIEVGVDVPNATLMVIEHAERFGLAQLHQLRG
                     RVGRGSEKSVCLLLRGEALSEVGRERLALMRATQDGFVLAEEDLRLRGGGELLGTRQS
                     GDTPFRIASLEQIQRLLPIAHDDARLLVERAGAEQGNALATPRGEAARILLYLFERDW
                     GVQLLRGG"
     gene            complement(21086..>21500)
                     /locus_tag="VFF98_11005"
     CDS             complement(21086..>21500)
                     /locus_tag="VFF98_11005"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64204.1"
                     /translation="TARPDAPLADILAAFLRPFLEVLAGTDEGWILYLRLIGRGMSNY
                     ARGGVHPALRQLAGIPDLLKEALRARYPLADEAAFHLASYMLEVALTHIVQDRGLLAA
                     RSEGQVEIARAIAHLDEISRYFAAGFAAIALRAES"
BASE COUNT         3810 a         7461 c         6653 g         3576 t
ORIGIN      
        1 tcccggtctt gagcgccaca tcggcggcat cctctatggc ctgctggata cgcccgcctg
       61 cccgtttcag cccccatgcc gaccggatgc gttcgatcac ctcatcgata tgcaccgggc
      121 cctcgatgct gaccgcctcc accagaaggg ccgtcagaat gcccgtgggc gtttcatgca
      181 gatcatcggt gcgatgggtc ggccggttca acaccgcctc cacataaagg ttctgtgggg
      241 gatcaccctc ctccccatcc acacgcacca gccccatctc ggtcacatcg tcccgttcga
      301 tgctgaagat atcgaccggc acggcgcgcg atgcccgctc atgcggctcg ccggcatgct
      361 cggccttcgc ctcctcgatc cgggcgatga tctgctcaag ctgctcattg ggccgatgga
      421 accagtcggt gctccatacg cggtgaatgg tccagccatg gctttccagc accgattgcc
      481 gcaggcggtc ccggtcacgc gcggaccgcg catcgtgata ggccgcaccg tcacattcga
      541 tccccagcag atagcggccc ggacgttcgg gatcggacac ggcaaggtcg atgaaaaagc
      601 ccgcaagacc gacctgacga tgcacatgat aaccgcgcgc ctgaagcgcc ttggcgacct
      661 gctcctcgaa cacgctgtca tgatcgcgcc cggtgctctc ggccatggtc atccggcccg
      721 tgcgcgcgaa atgcaggaac aggcgaaagg cgaacacacc cttgcggctc tgcgcaaagt
      781 ccgggtcgat gtcctcatcg gtcatcgagg aaaagacctc gcagcgctgc ttggcccggc
      841 tgatcaacac gttcagccgc cgctcgccgc cctccatgcc caatgggccg aaacgcatcg
      901 gcacccgccc gcccggcgtc gtggggccgt agccgaccga gatgaagatc acatcccgct
      961 catcgccctg cacattctca aggttcttga cgaagaaggg ctcagcataa tgcgcctgga
     1021 aaaaagcctc cacctcgggc gttgccgagc ggcgcagcag ttcgagatga tccagaatgg
     1081 cccgccgttg cgacacggag aaagccgcaa cgcccagcga cagttcggga tgatcgcagg
     1141 catgggccat gatcgccctg gccacgatct tcgcttcgac ctgattggtc ttggtgctgc
     1201 ctgaatcgaa caggccttcg gtgatatggt ggaaccgcag ccccattccg gcttccgccg
     1261 tataggggct gggcacgatg aacagcttgc tctcgtaaaa ctggcggttg ctgaccgcga
     1321 tcagcgactg atgacgactg cgataatgcc agcgtagcat ccgttcgggc aggccgcgcg
     1381 ccgtgaacag gccaagaatg ctctcgatat cggccacctg cgcgccgcca tcgtcatcgt
     1441 cgccatccgc gccattgccc gtcaccttcg agaaaaaggc cgttggcggc aactgcttgg
     1501 gatcgccgac cacgacgacc tgtctggccc gcgccaccgc gcccagcgca tcgacgggct
     1561 ggatctggct ggcctcatcc atgaccagca aatcgaattc gaaagccccg ggcgccagaa
     1621 actgcgcaac agacaggggg ctcatcatga agacgggctt caatgcctgc accgccggcc
     1681 cggcccgttc catcagcctg cggatcggca tatggccgcg cttgcgctcc atctccgtct
     1741 tcagcgtgcc cagcgggccg accggcggac cgccgttgcg cgggggaata cgctggtggt
     1801 gggcccgcac gacctccagg ctggccgcca cgatccgctg acggtccaga tcggcaaact
     1861 cgcgcgccag acggccatgc aatgtgccgt cgaaccggcc aagctcggga tgctcgcgca
     1921 actggtcctg atggatcgcc tcgaaatagg ccatttcaaa cgccgggatg acggccccgg
     1981 gcgccagccg tccgccctcc agccgcgcga ccacatcgcc gcagcccagc gcccgcgcgc
     2041 gatccgcgcg atcccgatag gccacccagc gcgacagatc ctcgccgccc gccgcccatt
     2101 ccgccagcct ctggcgcaaa tgggcacagg gcgccgccgc cacgctcgaa aagccgagca
     2161 ccacataggt ttcgcacgaa agctcgccca gcagcccgcc gatatcggcg acaagcgcat
     2221 cgccatcccg ttccagcgca tcggcactcg gcaccagcgc ctgtcgatgc gcgatccggc
     2281 tggccagcag gcggatgtca cgatggctct cgatccagtc cgccatgacg cgcaactcgc
     2341 cccaatccga cgtgcccccc tgccacatgg tatcgaacgc cgcctgacca aggtcgtcgt
     2401 cccgcatgag gctggcggaa cagcgctgtc cttcggcaag atcatcgagc gtccgaaggc
     2461 gaagcgacag atcagccggg accgtcgcca tggcggcagc actggcctga ccggcgtcat
     2521 gaaaaccgga aaccgccgcc agcaaggccg ccatatccgc gcgctccgcc gacaaggcat
     2581 cgccgaacat caccccttgc tcgggcagat cgctccacag cgcatcgatc accggcgaca
     2641 gttcggtcag cagggcctgc gtgcgggaca gacgtaaagt gatggcatcg cgatccgggc
     2701 gattggccgc cacgatccgg ggttccgccc ccagaccttt aagagaccgc acccattcga
     2761 tcagagcaag cagcgaagcc gaggccgaac ggtccccgcg ccagtctccc ccgaaagcgc
     2821 tgcgggcaaa ggcttcctcg gcctcgatcg cgcgccgcgc cgcctgccct tcggccaggg
     2881 catcgagctg ccccaacgtc tcgtccagcc cctgatccgg attggtcagg acagtgcgga
     2941 ccagccggtt ggcatggcgc caatcgccgc tcaagacctt gaaaaagccg gtgccatgcg
     3001 aggccagtgt cgcccgcgcc gcgcccagat ccatccccca tgccgcatcg agcagcctgt
     3061 caccgatggc cgtgcgggcg ctttccagcc gctgaaccgc cccggcgata tcagcggccc
     3121 gctcgacccc attgtcccac agatcagcgg caaaggcatc cgcgctggcc tctggcgcaa
     3181 cgaccacgcg gtcgcccacg cgcaacaggc gcctgatgtc ggccaatgtc gccggagcat
     3241 cgcgtcccat ggcttgcgaa agcgcatccg cctcggcccg caagcgtggt atatgctcca
     3301 ccagcaccga caggcgcgcg aaagcctcaa gcgaaaaacc ggtaggcaaa tgccccagcc
     3361 gatcccgcgc cccgacaaaa tccatggccc atgccgcatc ggggcagatc ggtgccagtg
     3421 cctcgcgcaa tgcggcgtaa cgcctgcccg tctccagcag cgcggcgatc tcctgcgcct
     3481 gctcttccca aaccggcgag cccagggcac gcgacgacag gccgggcgcc gtgcccaccc
     3541 gccgggccag cccggtcagc acacccacat cccgcaatct ggccacggcc tcctgctcca
     3601 gccgcgccgc aagcgcagcc tgatccgcat cgaagcggtc cagtcgctgg cccagtgcgg
     3661 caaagcggct cacaaaccgc tccacctcca tcggcgaggc cgaggccagc cgtaccccgc
     3721 gccagacatg atcggcaggc tggccgatca ccgccacccg ttcgaccagt tcggacagga
     3781 tgttatggcg ctggttgaaa ccatcggccg accatgtctg gggcgcctca agcgcaatat
     3841 cgttgggctg ctcccccgcc agccgcaacc gggccagttg tccgatcacc tgataggggg
     3901 tgagcccgct ggccgcgtga aggctgtgca tgcgcgcggc atgatcgttg agctgatcgc
     3961 gcgcctcggt caggcgggca tggagcgagc cgggatcgcc gcccttgggc gcgcccagtt
     4021 cccatgtccg gcgcaattca tccagcacgg cgcgcttgtt cgccttgttg ctgtgcagtt
     4081 ccagacaggc atcgccaacg cccgtggcat ccagcctgcg cttcacgacc tcgagcgccg
     4141 ccattttctc ggcaacgaac aggaccgtct ttccatcggc cacggcggaa gcaacgatgt
     4201 tggcgatcgt ctggctcttg cccgttcccg gcggcccctg gatcaccatg tcgcggccac
     4261 ggcgcacctc atgcacggcc agcgcctggc tgctgtcact gtccagaata tggagcatgt
     4321 cggcaggcgg gatgaaggga tcgatcttgg catcctcggg gatcatcccc ccggcgccct
     4381 ggaacccgtc ggccagcagg ccgcgcacga gcggccggtc gctgatccgc gcatgatcgg
     4441 gccagtttgc cggatcgaga tcgcgataca tcaggaattt ggcgaaagag aaaaagccca
     4501 gcgccatatc gtcatgcagc accgcccagc ccgtcttggc ggccaccgcc tcggcgacct
     4561 cgtccatata ggtcatcggg tcgaaaccat cgctcgcctc gaaagcgggc aggcggatgc
     4621 cgtgaacccg gtcgagccag gcctccagcg acaggttgga agcaaactcc tcgggccgcg
     4681 cgcgcagctt gaacttctcg ccggcattgc cacgttcgag cgacaccggg atcaacagca
     4741 agggcgcatg gcggatgttg gcggcattgc cgggatcgac ccagcgcagc atgccaagcg
     4801 tcagatagag gatattgacg ccctgctcct cctccagcgt gcgcgcgtcg aaatagagtt
     4861 caagcaggcg cttctgaagg cccttgggcg tcagccgggt ttgcaggcgg gtgtcggaat
     4921 ggcggctgaa aacaccgcgc tcgtcggcga catcatcctc gggaagggca agatccatga
     4981 tctcgtcggc gtcatcggtt tcgccctcat ccccgccgcg cgcgcccttt ccggcaagga
     5041 aggtaaagac cttgccttcc cgcaccagca ggcgaaagat ctcggcgctt ttctcgtcga
     5101 caatctcgac ggatcgcgcg tttttccgcg gcaggttgag caaccggttg cgcgccgaaa
     5161 ggtccagcaa ttccatgcgc gcgcggtcca gcttgtccgc aatcggcagg ttgctttgaa
     5221 agatcgacgc cggatcggca tcagactgag gaatcatagt cgtggccatg gagcaccccc
     5281 tacggccatc gtgctaagta tatcttacta agtctagcga tgaatcattg acatgacgct
     5341 ctgcatcagg gcgtcaagtc ccgtctccga aggcatcgga tgacatcgag cacatccgtg
     5401 ttcgttcgca gggccgcgac catataaaca caaaatgctt ccgcgtcgga aaagacgacg
     5461 aaagccatca actgctcgcc cttgtgtcgc aaatgccagg ccttgccgcc caccgaccac
     5521 gcctgcccaa aagcgccggt gcgaggatcg cggaaatgca ccgatgcgtc gcatcccaca
     5581 ccaagcgcat tcgaatgagc gtcgtatccc cacggcccaa gggcgacata attttcctcg
     5641 ccgttcttcg atctgtccgc aggcaaggtc cagagaccat caactatttc cgacgctggt
     5701 gctccgaaca actcattgct gcgtgcagcc gtcagcagca gcagaaaacc ccgccgataa
     5761 actagcggtt catcgaccag cgcccgcata agccagacga tctcatcgtc attgagaaaa
     5821 cgcttgttcg ccttcggccc ggtcgagaac cagttcgaat tgagcgtcta cgcaggatgc
     5881 gccatcggct cgatccccgc catgcgccct tcccgccccg cacaccattt caggaagcga
     5941 ctcagttacc cagccatttt gttagcccgc acctttgaag ccttggcctt gtcatagacg
     6001 gaatcccagc actcgttctc gctgagttgg tacaaggaga cgctaccaag ccgaggctcg
     6061 atatcgcggc tgaagatcac ctccttgtcg caaagggtcc tgggcttgag cttcctgcga
     6121 tcacctcgcc gcatcgcttc catgtaaatg gtatgcgcca tggcgaccgc aagcacctct
     6181 tgaacctcct tgacccgcag agccgccctg ggatcctccc ctcgctcaat tacctcattg
     6241 agctttgcag cccaattccg ggcctcggaa atcggatagg cgggaaggtc cccagcgtca
     6301 tgctgacaat cgccccggaa cgcgccaccc ggcgccgaaa taatcaggtt tttccgccgg
     6361 aagccgaaac gctgacacaa agtcccggca caagcggatc tgccagtcgg cctgcgatga
     6421 gcccatcaag gctcgaaggg ctgaagttcc gggtgcttcc catgatgacc tcgacacatg
     6481 cgaggcgata aaacgatgac acaaatcatg acacaagagg tgtccaaaca gatcgaaatc
     6541 tagcgaaatc tgaccagatc gacatctgga aattcggtct gatctggaac cctctatcac
     6601 cccgtaaaca gcggaaatcc gctaaaaatg caaggtttgg aaaaggttgc atcatggtag
     6661 cggaggaggg actcgaaccc ccgacacgcg gattatgatt ccgctgctct aacctgctga
     6721 gctactccgc cataccattc cggcgtctcc gaagaggcct tcctgcgctg ggcaggccgc
     6781 gctcttagga gcatgcggga aggtggtcaa cgggaaaaat gagaaaattt cgaagaatgt
     6841 gcaaagcgcc cgaaccacag cgccaccccg ccccgcctac agttcgcccc gcgcccggcg
     6901 gatggcaaac cagcgcgcca cattgcggtt atgctcgtcc agcgtggtgg caaacacatg
     6961 gccgccgctg ccatcggcca ccatatagag cacttccgtc ttggccgggt gcagcaccgc
     7021 atggatgctg gcgcggccgg gattggtgat cggcccatcg ggcaggccgg ccatggcata
     7081 ggtgttatag ccgttcaccg cgtggatctc gctttcgcgg atgcgccggc ccagcggctt
     7141 gcccttggtg atgggataga tgatggtcgg gtctgcctgc agcatgatcc ccttcttcag
     7201 ccggttctca tagaggccgg ccaccatcgc ccgctcctcg ctcttgccgg tctccttctc
     7261 gacgatggag gccagcgtca gcgcttcggc cggggtcttc accggcaggt cgggcgcgcg
     7321 ggcggcccat tcctcggcca gcgccttggt catcgccgcc tgcatgcggc ccaccacttc
     7381 ggcgcggctt tcgccccggg caaaatcata ggtttcaggc aggatgctgc cttctgccgg
     7441 cagcggcgcg gggccggcca gcatcgtctc gctcaacagc ttttcctgca ccatcacgct
     7501 gggcaggcct tcgggcacgg tgacgaagcg gcgcagcgca ttgtcgccgg tgatgatcgc
     7561 cagcacctgc gattcgctgg cgcccgccgg cagcaggaac tcacccgcct tcagcttgcg
     7621 gcccatcgcc agcagcttgg catgccagcg aaagttgaac gccgagcgca ccaccccgcc
     7681 cttttccagc cgcatggcaa cgctggcaag gctggcgcca tcgggcacgg tcacctgcgt
     7741 atcggccggc agcgggccgg gggcccacca gctccaggcc aggttcagcg ccggcaggcc
     7801 caccgccagc gccgcgccga tggcaatcag gcggcggcgc tgcttgcggg tcatgtcaga
     7861 gcgccttcac caccaggctg gcattggtgc cgccaaagcc gaaggaattg ttgagcgcgg
     7921 cgcgcacggg gcgcttcttg gcggtgtgcg gcaccaggtc cacgccctcg cagctctcgc
     7981 tggggttgtc gaggttgagg gtcggcggca cgatctggtc gcggatggcc aggatgcaga
     8041 agatcgtttc caccgcgccc gcgccgccca gcagatggcc gatcgcgctc ttggtggagc
     8101 tcatcgagac agtggagatg gcatcgccaa acaggcggcg caccgcgccc agttccagct
     8161 cgtcacccat cggcgtgctg gtgccatggg cgttcacata gtcgatgtcg gccggcgtca
     8221 tgcccgcctt cttcagcgcc atctccatcg agcgataggc gcccgagccg cccggatgcg
     8281 gcgccgtcac gtgataggca tcgcccgaga ggccatagcc caccacttcg gcatagatct
     8341 tggcgccgcg cgccacggca tgctcatatt cctcaaggca gaccacgccc gcgccctcgc
     8401 ccatgacgaa accgtcgcgg tccttgtcgt aggggcggct ggccttctcg ggctggtcgt
     8461 tataggcggt cgacagggcg cgggcctggg caaagccggc gatgccgatc ggacagatgg
     8521 tcgattcggc gccgccggcc agcatgatgt cggcatcgcc atcgcggatc atccgcgcgg
     8581 catcgccgat cgagtgggca ccggtggagc aggccgtgac gaccgcgtgg ttcggcccca
     8641 tcaggccata tttgatcgac acctgacccg agatcaggtt gatcagccgc ccgtggacga
     8701 aatggggcga gacgcgcgcc gggccctttt cggccagcac cagcgattcg ctctcgatac
     8761 ccggcaggcc gccgatgccg ctgccgatgc tgcagccggc gcgcaggcgc atctcttcgg
     8821 gcatgtcgac aaggcccgca tcttccagcg cctggccggc cgcatcaatg ccaaagacga
     8881 tgaacggatc gacctgccgc tggatcttgt gatccacccg cttgcccgca tcgaaaccat
     8941 attcatggtc ggccggcttc acttcgcagg cgatatggca cttctgccct gtggtatcga
     9001 acctggtgat gcgccccgcg cccgacttgc cggccaggat attggcccag gcggtttcga
     9061 catccgcgcc cagcggggtg acaagaccaa gaccggtgac aacaacacga cgcatcacga
     9121 cattctccac gcggatttga taccgcttca ttcatacgca acaggcccag ccctgacgga
     9181 ccgagcctgc ctcttgcccc gcgtttcgga ggccgaaggg ggcggattgc ccgccccatc
     9241 ccgtgcaccc gccgcagcca tgatccatgg ccagcgcgag acaaaccggg cgggctgcgc
     9301 ctttagccgg cagcggcccg cccgacaagc agaagcgctt agcccttgtg ctcgtcgata
     9361 tacttgatcg catccgaaac ggtgctgatc ttctcggcgg catcgtccgg gatctcaacg
     9421 ccgaactctt cctcgaaagc catcaccagc tcgacgatgt cgaggctgtc ggcgcccaga
     9481 tcatcgatga agctggcatc ttcggtgacc ttctcggcct cgacgcccag gtgctcaacg
     9541 acgatcttct taacgcgctc ggcggtgtcg ctcatggtaa tcccctttat gaagcggcaa
     9601 aacaggaaac tcgatgcgtg ccctaatggg tggcaagcgg gctggcaagc ggcgtttgcg
     9661 atagccgcca gcgcacaggt ggcaaagcgc cgggcccaaa cgggccccgc cccttgccgc
     9721 aaggcggcca gcctttcgcg cgcctagccc ttgggctgtt caaccacgcg gatgtgcaat
     9781 tcgcgcagtt gcttcatggt agccgcgctt ggcgcgccca tcaacaggtc ctctgcgcgc
     9841 tggttcatcg ggaacagcgt gatctcgcgc agattgtggg cgccgcacag cagcatgacg
     9901 atgcgatcca ccccgaaagc ggccccgcca tgcggcggcg cgccatactg gaaggcgcgg
     9961 tagagcccgc caaagcgcgc ttccacatcc tcgcggctaa ggccggtgat ctcgaaggcg
    10021 cgcaccatcg tctcgggcag atggttgcgg atggagcccg aggcgatctc gtagccattg
    10081 cacaccgcat catactgcag cgccttgatc gccagcggat cgccagcgtt cagcgcctcc
    10141 agcccgccct gcggcatgga gaacggattg tggcagaaat ccaccttctt ctcgtcctcg
    10201 ttccactcgt agaaggggaa gtcgacgatc cagcacaggc gataggcgtc cttctcgata
    10261 agatcgagca gctcgcccac gcggatgcgg gcagcgccag ccagcttggc ggcctgctcg
    10321 ctcttgccgg cggcgaagaa caggccgtcg ttctcgccca ggcccagctc ggcatagagc
    10381 ttttccatgc cctcggcccc gtggttcttg gcgatcggcc cgccaaactc gccgcccttg
    10441 cgggtgacat agccaaggcc ggcatggcct tcggcgcgcg cccactcgtt catctcgtcg
    10501 aagaacttgc ggctcttttc agcggtggcc ggcgccggaa tggcccgcac cacgccgccc
    10561 gagccgacga tcttctcgaa cagcccaaag ccggaactgg tgaaatgatg cgacacatcc
    10621 gagatgatca gcgggttgcg cagatccggc ttgtcggtgc catatttcag catcgcctca
    10681 tcaaagggga tgcgcggcca ttcgccggcg ggcgtaaccg ccgcgccctg gccgaactgg
    10741 gtgaagatgc ccgtcaccac cggctccagc gtctcccaga tctcttcctg ggtgacaaag
    10801 ctcatctcga ggtcgagctg gtagaattcg ccgggcaggc gatcggcgcg cggatcctcg
    10861 tcgcggaagc acggggcgat ctggaaatag cggtcgaagc cggccaccat cagcagctgc
    10921 ttgtactgct ggggcgcctg cggcagcgca aagaagctgc cgggatggat gcggctgggc
    10981 accagaaaat cgcgcgcgcc ctcggggctg gaagcggtca ggatcggcgt cgaatattcg
    11041 gtgaagccgg tgtcttccat cgcccggcgc atggccgaga tgaccttggt gcgcttgacg
    11101 atattggcgt gcagcgtctc gcggcgcagg tcgaggaagc ggtatttgag gcggatttcc
    11161 tcaggatatt cctgctcgcc ggccaccggc atcggcagct cggccgcggc cgactgcacc
    11221 gtggccacgc gcgcgaacac ctcgatcgcg ccggtggcaa gattggcgtt caccgtgccc
    11281 gcgccgcgcg ccttcaccac gccatcgatc gagacgacgc tttccacccg cagcgattcg
    11341 agcaccggaa gcgccgcaga atcgctgtcg gcaacgatct gggtgatgcc gtaatggtcg
    11401 cgcaggtcca caaacagcac gccgccatgg tcgcgctttc tgtgcaccca gcccgacagg
    11461 cgcacgttct ggcccacatg atcctgattg agcgccccgc aagtgtgcga acgataggga
    11521 tgggccagcg tgtccgtgac aggcgattgc atgacaagtc tttccattcg gccctaactc
    11581 gggcagggga acagggccgc gaccaaaggc gatgggcgcg gatcgtgcgg gctaacagtc
    11641 gatacccggc gatttgtcaa gccgcgggcc cttccggccc atcctccggc tgctttatgc
    11701 ccccttttgc aagggggcgc atgaagcggc gaaagcgccc gccaccgggc cagatgaaaa
    11761 gacataaaga cccaacgcat gaaaatccat ccgctgatca ccaccaccga tgcccttgcc
    11821 gatctgtgtg gccgccttgc cagggcgcag ttcgtctgcg tcgacaccga attcatgcgc
    11881 gagagcacct attggcccga gctgtgcctc atccagatcg ccgacgacaa ggaagccgcc
    11941 gcgatcgacc ccaaggccga tggcatcgac ctctcgccgc tgctcgacct gctggtcgac
    12001 aatgaggagg tgctgaagat cttccacgcc ggcgggcagg atgtggagat catctacaac
    12061 ctcaccggca agacccccca ccccatcttc gacacccaga tcgccatgat ggcgatcagc
    12121 cagagcgagc agatcggcta ttccaatctg gtggaaagct ggctgggcct cgtcatcgac
    12181 aagggcgcgc gctttaccga ctggagccgc cgcccgctga ccgagcggca gatcgaatat
    12241 gccatcggcg atgtcaccca cctcgcccgc atcttcccca agatcctcaa gcgcctgatc
    12301 aagaccggcc gcggggaatg gctggatgcc gagatggaaa agctggccga tccggaaaat
    12361 taccgcaacg atgccagcct ggcctggctg cgcatcaagg cggccgggcg caatccggcg
    12421 atgctgggcc ggctgaaggc gctggccgcc tggcgcgaat atgaggcgca ggacaagaac
    12481 atcccgcgcg gccgcatcgc ccgcgacgaa accctggccg acatggccag ccacccgccc
    12541 aaggcccagg cagacctcgc caaggtgcgc ggcctgtcgc ccggctggaa ggacaatgag
    12601 atcggcaagc ggctgatgct ggcgctggcc gatgcccagc cgctggccga ggacgagctg
    12661 cccccgcgcg cgccgcgcgg cgcgcccctg ggcaaggaag gctcgctggt ggcggatctg
    12721 ctgaagctgc tgctgaagat ccgcgcgcgc gagatcgatg tggccgcccg cctcctcgcc
    12781 cgctccgacg atctggaact gctggcggcc ggccagcgca agggcatctc catccttgaa
    12841 ggctggcgct atgaggtgtt tggccatgat gcgctggatc tggtggaggg caagctgggc
    12901 tttgccgttg tccacggccg gctgaagatg acccggctgg acgagagcgc cgacaccccc
    12961 gagggcgccg cctgaggatg agcgtctacc agccgaccct caagcagctg caatatctgg
    13021 tggcgctgca tgaacatggc catttcggcc gcgccgccga ttcctgcttt gtctcgcaat
    13081 cgacgctgtc ggcgggcctg cgcgatctgg aaagcctgct gggcgtaacg ctggtggaac
    13141 gcaccaagcg ggcggtgcat ttcaccccgc tgggcaatca ggtggtggcc aaggcccacc
    13201 gcctgctgcg cgaggcggag gagctgtcgg atctgatcca ggcctcctcg cagccgctgt
    13261 gcggtgaaat ccgcatgagc gtgatcccca ccatcgcccc gttcctgctg ccgcgcatgc
    13321 tgccccggct gcggcaggag cgccccgccc tcaagctctt cctgcgcgag gagccttcgg
    13381 gcgcggcgat cgaatcgctg cagcatggcc gggccgattg cgtgctgctg gccctgccct
    13441 ttgccaccgg cgaggtggaa aaggagacga tcgagctaga cgcgctctat gtcgccttcc
    13501 ccaaggacga tccgcgcgat ccgcccgagg agatctcccc cgacctcatc gacgagcacc
    13561 gcctgctgct gctggaagat ggccattgcc tgaaggacca tgcgctggcc gcctgcaacc
    13621 gcccggaact gcgcgccagc gccacgatga tcggcaccag cctgcacacg ctggtgcaga
    13681 tggtggacaa tgggctgggc ctgacgatgc tgccggagat ggcgctggat gcgggcattc
    13741 tcaacggcac caatgtggtg gcgcgcccgc tgcaatcgcc caccgccaac cgcgagatcg
    13801 cgctggtgtg gcgccgccat tcgccccgca ccgaggagtt ccgcctgctg gccgaggagc
    13861 tgcgcgccgg ttgatgcgag gcattatcaa cgccacgcgc cggaaattca tatagaattc
    13921 atcctgtctc ggcgaaacgg ggcccgccat tcccggtttt ttacaggaga ttacccatgc
    13981 gccgtatcac cctcgccctt gcggccctgg ccaccaccgc ggcctttgcc gccccggcca
    14041 tggcccaccc gcacaagcag ccccacaagg ccgcccagca cgggaaggcc gcccatggcc
    14101 atggccaggt gaagaagggc caccactgat cctcaggggt gctcataaag gaaaggccgg
    14161 tggagcgatc cgccggcctt ttttgcaggt tcagtccatg tgcttgaggc ctacccgcag
    14221 gtaatcccag ccggtgatca gcgtgaggcc ggccgcgccc cacagggtga tcagccccac
    14281 cgtatggggc acattggcct ctatccccca caccaccaca ttccaccagg gcatcgccac
    14341 gccgagaatc agcgcgccaa ggcagatgat ctggaaagtc gtcttccact tggccagctt
    14401 cgagaccggc accgacacct gaatgccgcc gagaaattcg cgcaggcccg aaacggcaat
    14461 ctcgcgcagc aaaatgatca gaccggcgat gacatgcaga tcctgcacat agggcccggt
    14521 gagcacgccc ttggccgtta acaccagaat caccgccgcc accatgatct tgtcggcgat
    14581 gggatcgagg aacacgccca gcttgctgac ggtgccttgc gcgcgcgcca gatagccgtc
    14641 gaaataatcg gttatgccca tcaaaccata catggcaaag gccaggccat agcccagcgg
    14701 ccagtccggc caccacatca gccagacgag gaagggcacc gcgagaatgc gcgacagggt
    14761 gagcaggttg ggcaaggtca gcatgacggg attcgccata gcggctcgtt cgcccacaaa
    14821 aaagacggaa ggtaggcttg cgggggcgcg atctgccgct agttagactg cctcgcctgc
    14881 ggacaaaccc ggaatatatg acccaatcga ccaaactgct gcgaggccgg cgcttcctgc
    14941 cgctgttcgt gacccaactg ctgggggcgt tcaacgacaa tctgttcaag aacgccatcg
    15001 tcttcttcgt cgtctacagc gtctatgatt cggacaaggc cgaggcgaaa ttctcggcga
    15061 tctcctcggc ggtgttcatc ctgcccttct tcctgctctc cgcgctttcg ggccagttgg
    15121 ccgacatgcg cgacaaggcg cgcatcatcc gcttcatcaa gctggcggaa atcggcatca
    15181 tgctggtggg ggcgtgcggg ctgctgctgg cctggcgggg gattggcacg caggatctgg
    15241 cgatcccgct gatgctgctg gccctgctgg gcatgggcac gcattccacc ttcttcggcc
    15301 ccatcaaata cgccatcctg ccccagcacc tggccgacaa ggaagtgctg gcgggcacgg
    15361 ggctggtgga agcgggcacc tatctggcca ttctggccgg cactatcctg gccggctgga
    15421 tcccggtgca atgggcggcc atcggcgtgg tgatcactgc gctgatcggc tttgtctcca
    15481 gcctggcggt gccttccgcg ccgccgctgg gggcgatcga gccgctcgat ttccatttcg
    15541 tgcgcgcctc catccggctg gtgcgcaaca cgatggaaga ccggcgggtg ttcctcgcca
    15601 tcgtggcaat cagctttttc tgggcagtgg gcgccatcct gttcatcgaa tttccgccgc
    15661 tggccaagaa tgtgctgacc gccagcaagg aagtggccag cctcttcctg gtgatcttct
    15721 cggtgggcgt ggcgctgggc tctgtctcga tcaacgcgct gctgaagggg cgggtctcgg
    15781 cccgctatgc gccggcctcg gtgctggtga tggccgcctt catcatcgct ttcgagcagg
    15841 tgtgccaggc ctggcagccg cagacagacg gcagcctgct gaccgttggc gcctttctcg
    15901 tccatccgct ggcgccggca ctgctgggca cgctggcggg cattgccatt gccggcggca
    15961 tgttcgtggt gccgctttat gccttcctca ccacctttgt ggaaaagagc cagaccgcgc
    16021 gcaccattgc cgccaacaac atcgtcaact ccggcgccat ggtgctgggc gccagcctgg
    16081 cggtggggtt gagcgcgctg ggcgtgtcga tcgtgcagca ggtgctgctg agcgctgcga
    16141 tgtgcctgat ctcggcctgg ctgtcctatc gcctgttccg cgccgaggac gatgggccgc
    16201 agcccgcctg atcagccgat aaacacgcat accgcgacga aacaggcgca ataggccatc
    16261 agaaattcgc gcaaggcccg cgcgccgcgc agcccctgca ccgctggcca ccagagcgcc
    16321 ggggcccggc ctgtgctcat ccgccgcaca tgcagcggca gcccctgacg gaaggggtgg
    16381 cgggcggcat cgtcgacaag gaccagggct ctgcgcataa gcgctgatta ggccccacag
    16441 atgccgcccg aaagtagtta atatggagtt aacaaactgc cccgtcccat agcgggacgg
    16501 gcgcgctcag tcttgcgaca gcttcaggat ataggcccat tcctccggcc gcaccgccgc
    16561 cacagacagg cgcgagagac gcaccagttc catttcggcc agcgccggat cggccttgat
    16621 ccgcttcagc gtaaccggat gggccagctt ttccaccggc ttcagcttca ccgccgccca
    16681 tttgccggtt tcgtcggtgg ggtcgctcag cccgcccacg ctgatggtgg cgatgccgac
    16741 gatctccagc ccgatgttcg aatggtagaa gaagaccaga tcgcccggct tcatggcgcg
    16801 caggttgttg gccgcgcggt ggttgcgcac gccgtcccag gtgccctcgc cctcggccac
    16861 cagatcgtcc cagctgtaga cgttgggttc ggatttcatc agccagtagt tgatttcgct
    16921 catgcctctt gtgtccattg cgcctatcgg ggaaggatcg agtgccttcc atagagccag
    16981 cgccccctgc acttcaaccg acccacggca tctggccggc cgaaaagacc cgaaaagaga
    17041 agcccatgcc ccattccctc gccgcgctcc ccgttctcag cctggcagcc ccccgcgccg
    17101 atttcgcccg cgacatcggc gccagcttcc gccgcttcgg ctttgcgctg gtggccgatc
    17161 atggcatcga tgccgccctc atcgccgagg gctggcggct gacggaagcc ttcttcgccc
    17221 tgcccgaggc ggaaaagcgc cgctggcatg tgcccggcat cggcggcgcg cgcggctaca
    17281 cgccgttccg caccgagatc gccaagggtg ccagcgcgca tgatctcaag gaattctggc
    17341 atgtcggccg cgatctggcc gacagcgaat atgccgccgg caccgccctt gccgcttcca
    17401 tgccgcgcaa cctctggccc gatgcgccag cccatttcgc cgacactttc cgcgcgctct
    17461 atgccgcctt cgatgcggcg ggcgcgcgca ttctggcggc cattgcgctc gatctgggcc
    17521 ttgcggaaga ctggtttgcc cctgccatcg atcagggcaa ttcggtgctg cggctgctgc
    17581 attatccgcc catcgccggg gccagctgcg gcgccatccg cgccggcgcg catgaggata
    17641 tcaacctcat caccctgctg ctgggcgccg aggaagccgg gctggaactg ctgaccaagc
    17701 agggcgaatg gcaggaggtg gccccgccgc cgggggccct ggtcatcaac atcggcgaca
    17761 tgctgcagcg cctgaccaac cacgtgctgc cttccaccac ccaccgcgtg cgcaaccccg
    17821 atgccgcgcg ggcagcccac agccgctatt ccatgccttt tttcctccat ctcaggagcg
    17881 atttcgcctt tcacacgttg cctcaatgca tcacgcccga aaatccggat cgctatccgg
    17941 tgtcgatcac ggcggatgat tatctgcagg aacgtttgcg cgagattggc ctgacaaaat
    18001 gacagacaga cccgggatcc tcatcactgc ccccctgctg ccgcagatcg agacggcgct
    18061 ggacgccgcc tttcccctgc atcgcctgtg ggaggcgccg gaccgcgcag cctttctggc
    18121 cgaggtgggc cccggcgtgc gcggccttgc cacggccggc tatttcggcc gggtgagcga
    18181 agatctgctc tgccagctgc cggcgctgga aatcatcgcc agcttcggtg tcggctacga
    18241 cacgatcgac accgccgccg ccaccgccta tggcgtggtg gtgaccaaca cccccggcgt
    18301 gctcgacgat gaggtggccg atctcaccgt gggcctgctg ctggccacca tccgccagat
    18361 cccgcaggca gagcgctttg tgcgcgaggg gcagtggctg aagggggcct tccccttctc
    18421 ggcctccctg cgcgggcgca aggtgggcat tctggggctg ggagccattg gcaaggcggt
    18481 ggcgcggcgg ctggaaggct ttggcgtgga gatcgcctat cacggccgcc atcgccaggc
    18541 tgatgtgccc tatgcctatc atgccagccc gctggaactg gcccgcgcgg tcgatacgct
    18601 ggtggtgctg acgcccggcg gcgcggccac gcagcatctg gtcaacgccg aggtgatcga
    18661 tgcgctgggg ccgcaggggg tgctggtgaa tgccgcgcgc ggcagcgtgc tggatcaggc
    18721 ggcgctggtg gcagcgcttc gcgaagggcg gctgctggcc gccgggctcg acgtgttcgc
    18781 tgaggaaccc tgccagcctg ccgatctcct cgccctgccg caggtggtgt gcctgcccca
    18841 tgtcggctct gccacgcaca agacgcgggc ggaaatgggc cagctgctgg tcgataacct
    18901 caccagctgg tttgccggca ccgggccgct gacaccggtg gccgaaagcc gcgcgctact
    18961 agcccccgcg taaaagctgc acgccccagt cgcgctcgaa cagatagagc aggatgcgcg
    19021 ccgcctcgcc gcgcggggtg gccagggcat tgccctgttc ggccccggcg cgctccacca
    19081 gcaggcgggc atcatcgtgg gcgatgggca gcaggcgctg gatctgttcg aggctggcga
    19141 tgcgaaaggg cgtgtcgcca gactggcgcg tgcccagcaa ttcgccgcca ccgcgcaggc
    19201 gcaggtcttc ctcggccagc acaaagccat cctgcgtggc ccgcatcagc gccaggcgtt
    19261 cgcgccccac ctcgctcagc gcctcgccgc gcagcaacag gcacaccgat ttttccgacc
    19321 cccgccccac ccggccgcgc agctgatgca gctgggccag gccaaagcgc tcggcatgtt
    19381 cgatcaccat cagcgtggca ttgggcacat ccacccccac ctcgatcacc gtggtggcta
    19441 ccagcacctg tgcctcgccg gcggcaaagc gggccatggc ggcatccttc acctcgggct
    19501 tcagctggcc gtggacgagt tccaccgccg cgcccagccg atcatgcagc agggcgtggc
    19561 gctcttcggc ggcggcaagg tcggcggttt cgctctcgcg caccatgggg cacacccaat
    19621 aggcctgccc gccgccggcg acatggcggg ccagcgccgt caacacatcg tccagccgct
    19681 cgatcgccac cacccgcgtg tcgatcgcct gccggccggg gggcagctcg tccagccggc
    19741 tcacatccat ctcgccatat tgcgccaggg tcagcgtgcg ggggataggg gtggcggtca
    19801 tcgccaggca atggggggtg cggcggccct tttgcgtcaa catcagccgc tggcccacgc
    19861 caaagcggtg ctgctcgtcg ataacggcca acgccagatt gcgataggca accccctcct
    19921 ggaagatcgc atgggtgccc acgatgatgt cgatgctgcc atccatcagg cccatgagga
    19981 tgctctcgcg cgccttgccc ttgtcgcgcc cggtgaacag cgccacccgg gcgccggtgg
    20041 gggcggccat cctcgaaagc gtttcgaaat gctggcgggc gaggatttcg gtgggggcca
    20101 gcagtgcccc ctgcccgccc gcttccaccg cgatcagcag cgcatgcagc gccaccaccg
    20161 tcttgcctga gcccacatcg ccctgcagca ggcgcagcat gggggcggtt tgcgccatgt
    20221 cgccctcgat ctcggcgatg gccctggcct gcgcgccggt gagcgcgaag ggcagcgcca
    20281 ggcgggcgcg caggcggcca tcgccgcgca ggggcaggcc ttcgcgccgg cggccctgct
    20341 ggcgcaccag catcaacgcc agcgcattgg cgaaaagctc gtcataggcg aggcggtcgc
    20401 gcgccttctt ggcctcggcg ctgtgggcct cggccagcgc cgcgcgccag gcgggccagc
    20461 cttcgcgggc cagaagcccc ggctcgatcc attcgggcag ctctggcagg cgggagagcg
    20521 cctgcgccac aaggccgccc agccgcccct gcgtcaggcc ttccgacagc ggatagaccg
    20581 cttcgcgctg ctggccggcc agcgccgagg cctctgccgc gacatgatcg gggtgcacga
    20641 tctgccaggc gccgccgaac tgatccagcc ggcccgccac ccagcgcgtc tcgcccagcg
    20701 gaaactgccg ggccgcccag ccgccattgc ggccgaaata ggccagcgcc acgctctcgc
    20761 ccgccccatc ctcggcaatg atgcgaaagg gcccgcgccc ggcgctgagc ttgtgggcgc
    20821 gcaccaccag cggcaggatc acctgatcgc caggcgcccc ttcggccagc gtggcgatca
    20881 cccggcgctc cacgaaacgt tcgggcaggt gataggccag gtcgcggatg cgcgtcagcc
    20941 ccagccggtc gagcgggcgg gcaagccccg cccccacgcc cttcaggctt tcgacagagg
    21001 caaacagcgg attgagcaga tcgggacgca tgatgccctt cccatagccg gcccaccgcg
    21061 tttttccagt ggcggcgcct ggctttcagc tctctgcccg cagcgcgatg gcggcaaagc
    21121 cggcggcgaa atagcggctg atctcatcga gatgggcgat ggcgcgggca atctccacct
    21181 gcccctcgct gcgcgcggcc agcagcccgc gatcctgcac gatatgggtg agcgccactt
    21241 ccagcatata gctggcaagg tggaaggcgg cctcgtcggc cagcggatag cgggcgcgca
    21301 gggcctcctt cagcagatcg gggatgcccg ccagctggcg cagggccgga tgcacgccgc
    21361 cgcgggcata attgctcatc ccccgcccga tcagccgcag atagaggatc cagccctcat
    21421 ccgtgccggc cagcacttcg aggaaagggc gcaggaaagc ggcaagaata tcggccagcg
    21481 gcgcatcggg gcgggcggtg
//
LOCUS       DASRYE010000217         8729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_61250,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000217 DASRYE010000000
VERSION     DASRYE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61250"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1316)
                     /locus_tag="VFF98_11010"
     CDS             complement(63..1316)
                     /locus_tag="VFF98_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZU64205.1"
                     /translation="MLTDAACRKAKPSPDGKPRKLPDALGLYLTIMPSGYKVWRFKYR
                     HAQKEGKLVIGPYPDVTLQDARAERDRVRALLRDGLNPSLEKKLRKMVTASEDLDTFE
                     LITRAWHKERAKTLSPRYAKQLLSRFEENVFPVIGQLPIKKITAPMILAVLRKIEARG
                     AKEMAKRVRGHISDVFSWAIGNALADQDPSAVVRKALIPADTGKRPALVDLQSARQLL
                     IKIHSAESAQVATRLASTLLALTAARPGVIRMAERHEFEQLNGPSPIWRISAEKMKLT
                     RQRKRDSAYEFLIPLAPQAVEIVQEAMQTSQSKRWLFAGVQKDAPISDNTLSKLYREN
                     GYAGRHVPHGWRATFSTIMNRIAAEANRPSDREIIDLMLAHIAGGVEPIYNRYLYMPR
                     RRQIACEWAAMLSNGLLPAASPTRF"
     gene            complement(1465..1539)
                     /locus_tag="VFF98_11015"
     tRNA            complement(1465..1539)
                     /locus_tag="VFF98_11015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Asn,seq:gtt)
     gene            complement(1708..2487)
                     /locus_tag="VFF98_11020"
     CDS             complement(1708..2487)
                     /locus_tag="VFF98_11020"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZU64206.1"
                     /translation="MTPPTKLALMASDTAQAQEAAAALAAEHAFVPLAEAEALVVVGG
                     DGFMLHALHHMMDEGHVVPAYGLNHGTVGFLMNKVKAGRPIGQRVAKARPIAVSPLHM
                     TATSNDGSEQSFYAINEVSLLRETRQTAKLEITVNGKVRLAELACDGVLVATPAGSTA
                     YNLSANGPILPLGSRMLALTPISPFRPRRWRGAILPESAEVIFRVLEPEKRPVAAVAD
                     QKEVRDISHVRIGAARDHSLTLLFDRSSSLEERIFSEQFQV"
     gene            2660..4030
                     /locus_tag="VFF98_11025"
     CDS             2660..4030
                     /locus_tag="VFF98_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZU64207.1"
                     /translation="MPMEDQSTVPGLAAEEGADAPRDALTGLPGMEAARQRLAGWLAE
                     GAVGPQGAEAGYVHALLLGVRRFDTINIAYGEAAGDGVLLALAERITRYAASELGGAV
                     MVARGPGGTFLILANEACSRERWQMFAEQLADLVALPIVRRSGTVRLSPRLALLRVVG
                     QEAPLSVFDRLAQTLEQAKRNLGRRVVWADGETTRVGRTAAQLEADLLRAIDRREIEV
                     VFQPQFALVGGADSAADPVVGAEALARWNHPKLGRIGAGALFAAAERADHVAPLSRHI
                     AQGALAAARGWPGHLRLSLNVTASDLAAASYAGEMAGLVAQSGFPAGRLTLEVTEQVL
                     LADMQLAERSLAALAGAGMRIALDDFGAGFCNFRYLKTLPLHYLKLDRSMVDGISEDA
                     RDLAVLRAIVAMARALDLAVIAEGIETEAQRLAVAREGCAIYQGFLRARPMRAAAFAA
                     LVGRGQ"
     gene            4124..4450
                     /locus_tag="VFF98_11030"
     CDS             4124..4450
                     /locus_tag="VFF98_11030"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HZU64208.1"
                     /translation="MTLLEENAEILREIAAGGTDLTQEVTLEFAHFFEDEEAAHAFAE
                     AAHGLAFAVEVFEGDEEEEEPWEVAASKVMVPTAEAITAAEQELGALAEKLGGEADGW
                     GFLEEE"
     gene            complement(4464..7955)
                     /gene="mfd"
                     /locus_tag="VFF98_11035"
     CDS             complement(4464..7955)
                     /gene="mfd"
                     /locus_tag="VFF98_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZU64209.1"
                     /translation="MPDIARILAATAPLTLAGLARGAQGLVLGDLARAAAMRKGRAVF
                     LAPDDAAMRALAEAARLFAPELTVIELPAWDCLPYDRASPALAISARRLAALHQLQAP
                     VAGPVLVLSTINAALQRLLTPFRIREQVRLLKPGMEISRESLAGLLRRQGYMRTDTVA
                     DAGEFAVRGAIVDIFPSGREEGLRLDFFGDELETLRSFDPATQRTSGTVPQHLLLPAG
                     EAMLDEDSIKRFRAGYRELFGANATADPLYQAVSDGRRMAGMEHWLPLFEERLATLFD
                     HLAPTDLLLADTSALGAAEERLGDIADYHQARRDPRQGGAYRPLEPASLYLTRAELDE
                     ALARWPAHKADAFAQPESASVIDFGFAGGRDFAPERARGDNAYEAAARHLQSAARAGR
                     KAVLACYSAGSLARITAILGEAVPPAPALADSWQEALGTAATGRVAALVLPLDTGFAN
                     ADIELVTEQDLLGDRLVRRKKKKKSADAFLAELAALTPGDLVVHLDHGIGRYEGLQSV
                     PVGGSPHDCVMLTYAGGDKLYIPVENLDVLSRYGSEAEGVALDRLGGEGWQKRRAKLR
                     ERIRAIAHELLATAAKRALRTAPALLPEQASFAPFVDRFPWQETEDQEAAIADVLDDL
                     GSGRPMDRLVCGDVGFGKTEVALRAAFVSAMAGCQVAVVAPTTLLARQHHKGFAERFD
                     GFPLQVGRLSRLVPEKEAALTRAGLADGTVDIVVGTHALLAKSVQFKRLGLVIVDEEQ
                     RFGVNHKERLKQLRADVHVLTLTATPIPRTLQMAMSGLRELSTIQTPPVDRLAVRTYV
                     MEWDEVVMREALLREHHRGGQSFIVVPRISDMAEVEDWLRAAVPEVKYVSAHGQMAAS
                     EVEERMSAFYERKYDVLLSTTIVESGIDIPSANTIIIHRADRFGLAQLYQLRGRVGRS
                     KLRAYAYLTTPAGRVLSEVAEKRLKVLGDLDSLGAGFQLASHDLDIRGAGNLLGDEQS
                     GHIREVGFELYQSMLEEAILAAKAGDYGLERESTRGLSPQITVDAPIMIPEDYVPDLA
                     VRMALYRRLNEVEEMSEIEALSAEMIDRFGPLPQPTANLVQLIQIKRQAIAAHIAKID
                     VGARGTLVAFHNDHFPDPAGLVAYAQRLEGTVKLRPDNKLVVNRAWGDPRARLNGLTQ
                     LTKGLSAIALKAQGKGR"
     gene            complement(8070..8348)
                     /locus_tag="VFF98_11040"
     CDS             complement(8070..8348)
                     /locus_tag="VFF98_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZU64210.1"
                     /translation="MVHISPDTARLKRIHYRAWHRGTREADYMIGGFFDHYHAGWGEA
                     DLVWFEALLDEEDVEIMGWALGSFVVPERYQGPLMEAFRRLDYVDIPR"
     gene            8482..>8729
                     /locus_tag="VFF98_11045"
     CDS             8482..>8729
                     /locus_tag="VFF98_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZU64211.1"
                     /translation="MPRQKGTDTGGTDARLAILDAAERIFANEGYNGAVIRDIAEAAG
                     VNLGLIQYHFGSKDKLFTAVINRRLDGWRARVAGSIAAA"
BASE COUNT         1421 a         2828 c         3032 g         1448 t
ORIGIN      
        1 ctgctgtgct gccgcgcagc ccgccgattg cccgggccgt catacgcggc ccccggcgat
       61 ggtcagaagc gggtggggct tgcggcgggc agcagaccgt tgctcagcat tgcggcccat
      121 tcgcaggcga tctgccgccg gcgaggcata tacaggtagc gattgtagat cggttctacc
      181 ccgccggcga tgtgcgccag catcaggtcg atgatctcgc gatctgaagg ccggtttgcc
      241 tccgccgcaa tcctgttcat gatggtcgag aaggtggcgc gccagccatg tggcacgtgg
      301 cgcccggcat agccattttc ccggtacagt ttcgacagcg tgttgtccga aatcggcgca
      361 tccttttgga cgcccgcgaa cagccagcgc ttgctctggc ttgtctgcat tgcctcctgc
      421 acaatctcta cagcctgcgg ggccagaggg attaagaact cgtaggctga atctcgcttg
      481 cgttggcgcg tcagcttcat cttctcggcc gatatgcgcc agatgggcga gggcccattc
      541 agctgctcga attcgtgcct ctcggccatc cgtatgacac ctggccgggc ggccgtgagc
      601 gccagaaggg tggaggccag gcgtgttgcc acttgggcag actcggccga atggatcttg
      661 atcagcaatt gccttgcgct ctgcaggtcg accagcgctg gccttttgcc ggtgtccgcc
      721 ggtatcaatg ccttgcgcac cacagctgac ggatcctgat cggccagcgc attgccgata
      781 gcccagctga acacgtccga aatatgcccg cgaacgcgct tggccatttc cttggcgcca
      841 cgggcctcga tcttgcgtag caccgcaagt atcattggcg cggtgatctt cttgatcggc
      901 agctggccaa tcaccggaaa cacgttctct tcgaagcggc tgagcagctg cttggcatag
      961 cgaggcgaca gcgtcttggc gcgttccttg tgccaggctc gcgtgatcag ctcgaaagtg
     1021 tcgaggtcct cggacgccgt caccatcttg cgcagcttct tctcgagcga cgggttcaag
     1081 ccgtctcgca gcagggcgcg cacgcgatcg cgttcggctc gggcgtcctg aagcgtgaca
     1141 tctggatacg gcccgatgac caacttcccc tccttctggg catggcgata tttgaagcgc
     1201 caaaccttgt agccggaggg cattatcgtc aggtacaagc ctagcgcatc gggaagtttg
     1261 cgaggtttgc catccgggct gggcttggct tttctgcagg ccgcatcggt cagcatgaag
     1321 gcccccattc ggggccccca agaaggcccc caaactgggg gcccttggat gagaaggggt
     1381 gcactcgcat gcgaaaggtg ttgccgctaa aggcgcagaa aatctaggtt tcgcgctcac
     1441 atgcaacggc atgcgaaaag gaaatggctc cctgagtagg attcgaacct acggccgctc
     1501 gattaacagt cgagagctct accgctgagc tatcagggat catttcgttc cgcctggcgt
     1561 cgttgccgcc gggcaggtgc gcgctaatag cagggcttcc ggccttggca agcggcaatt
     1621 gcaattttct gacaaggcga aatcaatata ttgaaaataa acgatataaa ctgaacgcag
     1681 ggcccccgcc ggggcgggcc cggcgaatca cacctggaac tgttcggaga aaatccgctc
     1741 ctccaggctg ctggaacgat cgaacagcaa ggtcagcgaa tgatcgcgcg cggcgccgat
     1801 gcgcacatgg ctgatgtcgc gcacttcctt ctggtcggca acggcggcca ccgggcgctt
     1861 ttcgggctcc agcacgcgga agatcacctc ggcgctttcc ggcaggatgg cgccgcgcca
     1921 gcgccgcggg cggaacgggc tgatcggggt gagcgccagc atgcggctgc ccagcggcag
     1981 gatcgggcca ttggccgaga gattataggc cgtggagccg gccggcgttg ccaccagcac
     2041 gccatcgcag gccagttcgg ccagccgcac cttgccgttc acggtgatct ccagcttggc
     2101 ggtctggcgc gtctcgcgca gcaaagacac ctcgttgatg gcatagaagc tctgctcgct
     2161 gccatcattg ctggtggcgg tcatgtgcag cggcgagacg gcgatgggcc gcgccttggc
     2221 cacgcgctgg ccaatcggcc ggccggcctt caccttgttc atcaagaagc ccaccgtgcc
     2281 gtggttcagc ccataggcgg gcaccacatg gccctcgtcc atcatgtggt gcagggcgtg
     2341 cagcatgaag ccgtcgccgc ccaccaccac cagtgcctcg gcctcggcca gtggcacgaa
     2401 ggcgtgctct gcggcaaggg cggcggcggc ttcctgcgcc tgggcggtgt cagacgccat
     2461 cagggcgagc ttggtaggcg gggtcatctg ggcagtcctc gttatgttcg ggcctgtttc
     2521 agccctgttg ccggaaatcg cccggcaaaa aggctttggc aagttgtctc acacggcgaa
     2581 gaagggcaaa gaaaggccaa gggcaagcat ggactggcaa acccggcaca aatatcagct
     2641 acgcaatcga acggacggca tgcccatgga agatcagtcg acagtgccgg gccttgccgc
     2701 cgaagagggc gccgatgccc cgcgcgatgc gctgacgggc ctgccgggca tggaagcggc
     2761 ccgccagcgc ctggccggct ggctggccga aggggctgtg gggccgcaag gggccgaggc
     2821 cggctatgtg catgcgctgc tgctgggcgt gcgccggttc gacacgatca acatcgccta
     2881 tggcgaggcg gccggtgatg gcgtgctgct ggccctggcc gagcgcatca cccgctatgc
     2941 cgccagcgag ctgggcgggg cggtgatggt ggcgcgcggg cccggcggca ccttcctgat
     3001 cctggccaac gaggcctgca gccgcgagcg ctggcagatg tttgccgagc agctggccga
     3061 tctggtggcg ctgccgatcg tgcggcgttc gggcacggtg cggctgtcgc cccggctggc
     3121 gctgctgcgg gtggtggggc aggaagcccc gctctcggtg ttcgaccggc tggcgcagac
     3181 gctggagcag gccaagcgca acctgggccg ccgcgtggta tgggccgatg gcgagaccac
     3241 ccgcgtgggc cgcaccgccg cccagctgga ggccgacctg ctgcgcgcga tcgaccggcg
     3301 cgagatcgag gtggtgttcc agccgcaatt cgcgctggtg gggggggctg attcggccgc
     3361 cgatccggtg gtgggcgccg aggcgctggc ccgctggaac caccccaagc tgggccgcat
     3421 tggcgccggc gccctgtttg ccgcggccga gcgggccgat catgtggccc ccctgtcgcg
     3481 ccatatcgcg caaggggcgc tggcggcggc gcggggctgg ccgggccatt tgcgcctgtc
     3541 gctcaatgtc acggcttccg atctggcggc ggccagctat gctggggaaa tggccgggct
     3601 ggtggcgcaa tcgggctttc cggccggccg gttgacgctg gaagtgaccg agcaggtgct
     3661 gctggccgat atgcagctgg ccgaacgctc gctggcggcg ctggcagggg ccggcatgcg
     3721 catcgcgctc gacgattttg gcgccggttt ctgcaatttc cgctatctga agaccttgcc
     3781 gctgcattat ctgaagctcg atcgctcgat ggtcgatggc atcagcgagg atgcgcgcga
     3841 tctggcggtg ctgcgcgcga ttgtggcgat ggcccgcgcg ctggatctgg cagtgatcgc
     3901 cgaggggatc gagaccgagg cccagcggct tgccgtggcg cgcgagggct gcgccatcta
     3961 tcaggggttc ctgcgcgcgc ggccgatgcg ggcggcggcc tttgcggcgc tggtgggccg
     4021 gggccagtaa ggtgggaacc caggtcatgc ctctgtcgtt tttccgtttt aaaaccttca
     4081 tccagccggg gtggcgcctg ctgcccgaat gtgaggaaca gccatgacgc ttctggaaga
     4141 aaacgccgaa atcctgcgtg agattgccgc tggcggcacc gatctgacgc aggaagtgac
     4201 gctggaattc gcccatttct tcgaggatga ggaagccgct catgcctttg ccgaggcagc
     4261 gcatggcctg gccttcgccg tcgaggtgtt cgagggcgac gaggaagagg aagagccctg
     4321 ggaagtggcc gccagcaagg tgatggtgcc caccgccgag gcgatcaccg cggccgagca
     4381 ggaactgggc gcgctggccg aaaagctggg cggcgaggcc gacggctggg gcttcctcga
     4441 agaggaatga gccccgcgcg ccgttagcgt cccttgccct gcgctttcag ggcaatcgca
     4501 gacaggccct tggtcagctg cgtcagcccg ttcagccgcg ccctgggatc gccccaggcg
     4561 cggttgacga cgagcttgtt gtcgggccgc agcttcaccg tgccctccag cctttgcgca
     4621 taggccacca gcccggccgg atcggggaaa tggtcgttgt ggaaggccac cagcgtgccg
     4681 cgcgcgccca cgtcgatctt ggcgatatgg gcggcgatcg cctgccgctt gatctggata
     4741 agctggacga gattggcggt gggctggggc agcgggccga agcggtcgat catctcggcc
     4801 gacagggcct cgatctcgct catctcctcc acctcgttga ggcgccgata gagcgccatg
     4861 cgcacggcca gatccggcac atagtcttcc gggatcatga tcggcgcatc cacggtgatc
     4921 tgcggcgaga ggccgcgggt cgattcgcgc tccagcccgt aatcgccggc cttggccgcc
     4981 aggatcgcct cttccagcat cgactgataa agctcgaagc ccacctcgcg gatatggccc
     5041 gattgttcat cgcccagcag gttgccggcg ccgcgaatgt cgagatcatg gctggccagc
     5101 tggaagccgg cacccagcga atcgagatcg cccagcactt tcagccgctt ctcggccacc
     5161 tcgctcagca cccggccggc cggcgtggtg agataggcat aggcccgcag cttggagcgc
     5221 cccacccgcc cgcgcagctg atagagctgg gccaggccaa agcgatccgc ccggtggatg
     5281 atgatggtgt tggccgaggg gatatcgatc ccgctctcga cgatggtggt ggagagcagc
     5341 acgtcgtatt tgcgctcgta gaaggcgctc atccgctctt ccacctcgct ggcggccatc
     5401 tgcccatggg ccgagacata tttcacttcc ggcaccgccg cgcgcagcca gtcttccacc
     5461 tcggccatgt cggaaatgcg cggcacgacg atgaagctct gcccgccgcg gtggtgttcg
     5521 cgcagcaggg cctcgcgcat caccacctcg tcccattcca tcacataggt gcgcacggca
     5581 agccggtcga ccgggggggt ctggatggtg gacagctcgc gcaggccgct catcgccatc
     5641 tgcagcgtgc gcgggatggg cgtggcggtc agcgtcagca catggacatc ggcgcgcagc
     5701 tgcttcagcc gctccttgtg gttgacgcca aagcgctgct cctcatcgac gatgaccagc
     5761 cccagccgct tgaactgcac cgatttggcc agcagcgcat gggtgcccac aacgatatcc
     5821 accgtgccat cggcaagccc tgcgcgggtg agcgcggcct ccttctcggg caccaggcgc
     5881 gagaggcggc ccacctgcag cgggaagccg tcaaaccttt cggcaaagcc cttgtggtgc
     5941 tggcgggcca gcagcgtggt gggggccacc accgccacct ggcagccggc catggccgag
     6001 acgaaagccg cgcgcagggc cacctcggtc ttgccaaagc cgacatcgcc gcacaccagc
     6061 cggtccatcg gccggccaga gccgagatca tccagcacat cggcgatggc cgcttcctgg
     6121 tcctcggtct cctgccaggg gaagcggtcg acgaaggggg caaagctggc ctgttcgggc
     6181 agcagggcag gggcggtgcg caaggcgcgc ttggcggcgg tggccagcaa ttcgtgggcg
     6241 atggcgcgga tgcgctcgcg cagcttggcg cgccgcttct gccagccctc gccgcccagc
     6301 cggtcgagcg ccaccccttc ggcctcgctg ccatagcggc tgagcacatc gaggttctcg
     6361 accgggatat agagcttgtc gcccccggca taggtgagca tcacgcaatc atgcggagag
     6421 ccgcccaccg gcaccgattg gaggccttca taacggccaa tgccatggtc gaggtggacg
     6481 accagatcgc ccggcgtcag cgccgccagt tcggcgagga aggcgtcggc cgatttcttc
     6541 tttttcttgc ggcgcaccag ccggtcgccc agcaggtcct gctcggtaac cagctcgata
     6601 tcggcattgg caaagccggt gtcgagcggc agcaccaggg cggccacccg gccggtggcg
     6661 gcggtgccca gcgcttcctg ccagctgtcg gccagcgcgg gggcaggggg caccgcctcg
     6721 cccagaatgg cggtgatgcg ggccaggctg ccggccgaat agcaggccag caccgccttg
     6781 cggccggcgc gggcggcgct ttgcaagtgg cgggcggcgg cttcataggc attgtcgccg
     6841 cgggcccgtt cgggggcgaa atcgcgcccg ccggcaaagc cgaaatcgat gaccgaggcg
     6901 ctttcgggct gggcgaaggc atcggccttg tgggcgggcc agcgggccag cgcctcgtcc
     6961 agctcggcgc gggtgagata gaggctggcc ggctccagcg ggcgataggc gccgccctgc
     7021 ctgggatcgc gccgggcctg gtggtaatcg gcgatgtcgc ccagccgctc ctcggccgcg
     7081 cccagcgccg aggtatcggc cagcaggagg tctgtggggg cgaggtgatc gaaaagcgtg
     7141 gccaggcgct cctcgaacag cggcagccaa tgttccatgc cggccatccg ccgcccgtcc
     7201 gacaccgcct gatagagcgg atcggccgtg gcattggcgc cgaacagctc gcgatagccg
     7261 gcacggaagc gcttgatgct gtcttcgtcg agcatcgcct cgccggcggg cagcagcagg
     7321 tgctggggca cggtgccgct ggtgcgctgg gtggccggat cgaagctgcg cagcgtttcc
     7381 agctcgtcgc cgaagaagtc gagcctcagg ccctcctcgc ggcccgaggg gaagatgtcg
     7441 acgatcgccc cgcgcacggc aaattcgccg gcatcggcca cggtgtcggt gcgcatgtag
     7501 ccctggcggc gcagcaggcc ggcaaggctc tcgcggctga tctccatgcc cggcttcagc
     7561 aggcgcacct gctcgcggat gcggaacggg gtgagcaggc gctgcagcgc ggcattgatg
     7621 gtggagagca ccagcaccgg ccctgccacc ggcgcctgca gctggtgcag cgcggccagg
     7681 cgccgggcgc tgatggccag cgccgggctg gcgcggtcat agggcaggca atcccaggcc
     7741 ggcagctcga tcacggtgag ttcgggggcg aacaggcggg cggcctcggc cagcgcgcgc
     7801 atcgccgcat catcgggcgc caggaacacc gcgcggccct tgcgcatggc cgccgcccgc
     7861 gccagatcgc ccagcaccag cccttgcgcg ccgcgggcaa ggccggccag cgtgagcgga
     7921 gcagtggcgg caagaatgcg ggcaatatca ggcatggcgg gcggcgatct tccaacaggc
     7981 aaaagccgcc cgcgccgaat cggcggggcg gtgaacaggc agcggcttag cccatgcggg
     8041 gccggcaggg aaaggtgggg ccgcggcgct cagcgcggga tatcgacgta gtccagccgg
     8101 cggaacgcct ccatcagcgg gccctgatag cgttcgggca ccacaaagct gcccagcgcc
     8161 cagcccatga tctccacgtc ttcctcatcg agcagcgcct cgaaccagac gagatccgcc
     8221 tcgccccagc cggcgtgata atggtcgaag aagccgccga tcatgtaatc ggcctctcgc
     8281 gtgccgcgat gccaggcgcg atagtggatc cgcttgaggc gagctgtgtc gggcgagatg
     8341 tggaccatgg gccaagcgcc taggccagtg cgcgccaaag cgcaaccgca acgggcctgc
     8401 gggcggcgcc tcttccattg atcaggcttt cgccggggcc tatcctgtgc tccgctggcc
     8461 ggaacagtgg aaaggagaac catgccccgg cagaagggaa ccgatacagg cggcaccgat
     8521 gcgcgccttg ccatcttgga cgcggccgag aggatctttg ccaacgaagg ctacaacggc
     8581 gctgtcatcc gcgatatcgc cgaggcggcg ggcgttaacc tgggcctcat ccagtatcat
     8641 ttcggcagca aggacaagct gttcaccgcc gtcatcaacc gccggctcga tggctggcgg
     8701 gcgcgcgtgg ctggcagcat tgccgctgc
//
LOCUS       DASRYE010000218         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_257280,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000218 DASRYE010000000
VERSION     DASRYE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257280"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFF98_11050"
     CDS             <1..216
                     /locus_tag="VFF98_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64212.1"
                     /translation="DRRAQGGYSLARTSGRWWVEGAQLCRQQARPAPDAPQCESAEAY
                     PRPGTSTASPDGQMLLILLKGWMEPIR"
     gene            complement(262..1953)
                     /locus_tag="VFF98_11055"
     CDS             complement(262..1953)
                     /locus_tag="VFF98_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZU64213.1"
                     /translation="MTHDFDAIVVGSGITGGWAAKELTEAGLKVLLIERGRDIPHQTG
                     YDREMKAPWELPFRGQGDQLAYADQYPVQRLNRHFTEWTENHFVNDRESPYTTGEDTH
                     FTWFRSYQLGGRSLTWGRQCYRWSDYDFGANRRDGHGTDWPIRYADIAPWYDHVESFI
                     GVSGAAEGLAQLPDGRFQPAMPLNAVEQACRAAIRARWPERVLTIGRTANLTEAKPEE
                     GRAHCQYRDICARGCSFGAYFSTQSSTLPAARKTGRLTLLTDKQVERLDYDPATRRIT
                     GIRWIDRTTRQRGQASARLVFLNAGAFNSVHLLLNSASEAMPQGLANGSGVLGTHIMD
                     HANTMAGMAEMPGFEGHTMFGNRPTGIVIPRFRNMEVLDGKGHTRGFSFQGGALQSGW
                     SRGKREAGLGAAFKDSLRGPGAWRMVLVAFADCVPRASNRLTLDRTRTDPLGLPQLKI
                     DFAFGQEEHAALAEAHEAAKEMLEAAGGRFLFGSTQPGSGGSAIHEMGGARMGHDPAT
                     SVLNQWSQAHEVANLFVTDGAQMASSACQNPSLTYMALTARAAAHAVELAKGGVI"
     gene            complement(1959..>2386)
                     /locus_tag="VFF98_11060"
     CDS             complement(1959..>2386)
                     /locus_tag="VFF98_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZU64214.1"
                     /translation="LALAHGLEGTRAPLAAPPPATKAPLPLRADGSPRYLAWLEAELD
                     ARAGGFLAASAAARQAALAALDAESFPPGPPLGPPAAFGPNPWCKVKALILTGYYTSE
                     EGAARELQYQLIPGRFDPDAPLHPGDRAWSSDWTAVDFG"
BASE COUNT          361 a          776 c          875 g          374 t
ORIGIN      
        1 gaccggcggg cgcagggcgg ctatagcctg gcccgcacca gcgggcgctg gtgggtggag
       61 ggcgcgcagc tgtgccgcca gcaggcccgc cccgcccctg atgccccgca atgcgaaagc
      121 gccgaggcct atccccgccc cggcacatcc acggccagcc cggatgggca gatgctgctg
      181 atcctgctga agggatggat ggagccgatc aggtaggttg cgaggggcca gccccccgca
      241 accacccgtt tctggcgcgc ctcagatcac cccgcccttc gccagttcca ccgcatgggc
      301 ggcagcccgc gccgtcagcg ccatataggt gagcgagggg ttctggcagg cgctggaggc
      361 catttgcgcg ccatcggtaa cgaagaggtt ggccacctca tgcgcctggc tccattggtt
      421 gagcacgctg gtggccggat catggcccat gcgcgcgccg cccatctcgt ggatggcgct
      481 gccgccgctg ccgggctggg tcgaaccgaa aaggaagcgg ccgccggcag cttccagcat
      541 ttccttcgcc gcctcatgcg cttcggcaag ggctgcgtgc tcttcctggc cgaaagcgaa
      601 gtcgatcttc agctgcggca ggcccagcgg atcggtgcgg gtgcgatcga gcgtgaggcg
      661 gttgctggcg cgcggcacgc aatcggcaaa ggccaccagc accatgcgcc aggcgccggg
      721 gccgcgcaga ctgtccttga aggccgcgcc caggcccgct tcgcgcttgc cccggctcca
      781 gcccgattgc agcgccccgc cctggaagga gaagccgcgc gtgtggccct tgccatccag
      841 cacttccatg ttgcgaaagc gcgggatgac aatgccggtg ggccggttgc cgaacatggt
      901 gtggccttca aagccgggca tttcggccat gccggccatg gtgttggcat gatccatgat
      961 gtgggtgccc agcacgccag agccattggc caggccctgc ggcatcgcct cgctggccga
     1021 gttcagcagc aggtgcacgc tgttgaaggc gccggcattg aggaacacca gccgcgcgct
     1081 ggcctggccg cgctggcggg tggtgcgatc gatccagcgg atgccggtga tgcgccgggt
     1141 cgccgggtca taatccagcc gttccacctg cttgtcggta agcagcgtca ggcggccggt
     1201 cttgcgcgcg gccggcaggg tggaggactg ggtggagaaa taggcgccga aggagcagcc
     1261 gcgcgcgcag atgtcgcgat actggcaatg ggcgcggcct tcctcgggct tggcctcggt
     1321 gaggttggcg gtgcggccga tggtcagcac gcgttcgggc catcgggcgc ggatggccgc
     1381 gcggcaggcc tgttccaccg cgttcagcgg catggccggc tggaagcggc catcgggcaa
     1441 ttgcgccagc ccttcggccg cgcccgacac gccaatgaag ctttcgacat gatcgtacca
     1501 cggggcgata tcggcatagc ggatgggcca gtccgtgccg tggccatcgc gccggttggc
     1561 gccgaaatca taatccgacc agcgatagca ctggcgcccc caggtgagcg agcgcccgcc
     1621 cagctgatag gagcggaacc aggtgaaatg cgtatcctcg cccgtggtgt aggggctttc
     1681 gcgatcgttc acgaagtggt tttcggtcca ttccgtgaaa tggcggttca gccgctggac
     1741 gggatattga tcggcatagg ccagctggtc gccctggccg cggaagggca gttcccaggg
     1801 ggccttcatc tcgcggtcat agccggtctg gtgggggatg tcgcggccgc gctcgatcag
     1861 cagcaccttg aggccggcct cggtgagttc cttggccgcc cagccgccgg tgatgccgga
     1921 gccgacgacg atggcgtcga aatcatgagt catgaggctt atccgaaatc cactgccgtc
     1981 cagtcgctcg accatgccct gtcgccgggg tgcagcgggg catcggggtc gaaacggccg
     2041 ggaatgagct gatattgcaa ttcgcgcgcg gcgccttcct cgctggtgta gtagccggtg
     2101 aggatcagcg ccttcacctt gcaccagggg ttggggccaa aggcagcggg ggggccgagc
     2161 ggcgggccgg gcgggaagct ttcggcatcc agcgcggcca gggcagcctg gcgggcggcc
     2221 gcgctggcgg cgaggaaacc gccggcgcgc gcatccagtt cggcctccag ccaggcgaga
     2281 tagcgcggcg agccatcggc gcgcagcggc agcggggcct tggtggcggg cggcggggca
     2341 gccagcggcg cgcgggtgcc ttccagccca tgggccagcg ccagcg
//
LOCUS       DASRYE010000219        14311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_154204,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000219 DASRYE010000000
VERSION     DASRYE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154204"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="fusA"
                     /locus_tag="VFF98_11065"
     CDS             complement(<1..1656)
                     /gene="fusA"
                     /locus_tag="VFF98_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZU64215.1"
                     /translation="MARSHPLEKYRNIGIMAHIDAGKTTTTERILYYTGKSYKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWNDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VACFDGVAGVEPQSETVWRQADKYGVPRMCFINKLDRTGANFKYCVQSIIDRLGAKPA
                     VLYIPIGLEADLKGLVDLVHNRAIIWKDEALGAEFFYEEIPADLADEAAEYRANLIEL
                     AVEQDDDAMEAYLEGTEPDVDTLKALIRKGTLNQSFVPVCCGSAFKNKGVQPLLDAVV
                     DYLPSPLDIPDVQGVKMDSDEKDSRPAKDDAPFSALAFKVMNDPFVGSLTFIRIYSGT
                     LTKGTYLNSVKDKKEKVGRMLEMHANERKDIDEAFAGDIIALAGMKETTTGDTLCAER
                     FPIVLERMEFPEPVIELSVEPKTKADQEKMGIALNRLSAEDPSFRVSTDHESGQTIIK
                     GMGELHLDIIVDRMRREFKVEANVGAPQVAYREYLAKPVDIDYTHKKQSGGTGQFGRV
                     KVKLSPGERGSGFVFKDEIKGGNIPKEYIPAIEKGFRETAATGS"
     gene            complement(1768..2238)
                     /gene="rpsG"
                     /locus_tag="VFF98_11070"
     CDS             complement(1768..2238)
                     /gene="rpsG"
                     /locus_tag="VFF98_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZU64216.1"
                     /translation="MSRRRRPEKREILPDPKFGDQVLSKFMNNLMLDGKKSVAESIVY
                     GALDTMQTRAKADPVQLFHDALNNVKPQIEVRSRRVGGATYQVPVEVRPERAQALAIR
                     WLITAARNRSETTMSARLSGELLDAANNRGNAVKKREDTHRMADANRAFSHYRW"
     gene            complement(2288..2659)
                     /gene="rpsL"
                     /locus_tag="VFF98_11075"
     CDS             complement(2288..2659)
                     /gene="rpsL"
                     /locus_tag="VFF98_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZU64217.1"
                     /translation="MPTINQLVRKGRELQKAKSKVPAMEQNPQKRGVCTRVYTTTPKK
                     PNSALRKVAKIRLTNSREVISYIPGEGHNLQEHSVVLIRGGRVRDLPGVRYHVLRGVL
                     DTQGVKNRKQSRSKYGAKRPK"
     gene            complement(2977..3813)
                     /locus_tag="VFF98_11080"
     CDS             complement(2977..3813)
                     /locus_tag="VFF98_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64218.1"
                     /translation="MGFGNPLIYRSSASGYELVPGQRVNRLGKITTINDLGGRGPALP
                     RQPSADRLRVLSIGDSVANGGTQINDEQTYPMLTGACLRTAGLPAEVLNVAAGGWAVQ
                     NEAAWLKAHGVFGARVVLLEVNEKDLDQAFGDASLLDRNPSFPSHRPATGLSEILFRY
                     ALPRFGLGVAADPGSTAGAFNAANVGAVMNAIGEIKSLSDAAGARMAILYWDPLMSGA
                     AGAGRAREQLFAWANGHGVQIIRPQLNRRRDAPGLFRDGIHPNERGNAIIASAICQAR
                     VI"
     gene            4525..5235
                     /locus_tag="VFF98_11085"
     CDS             4525..5235
                     /locus_tag="VFF98_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64219.1"
                     /translation="MEALYRLRHQPGAQGFAAAWEAAVDRGMARLEDCALERAIAGEE
                     RVMIRRGEVVARWTRYDTPLLVFLLRNRRRGRFGQEALLSRAEWEAVREAEEAGEAEV
                     VLERLNAKLDAMRARMAAAAETAAAEEDATPGEEGAGEEPADDQAAGPAGGRALEVKG
                     LAGMEAACGAQPAGGAARMLPGFLRQGDAGVGMPRIDPGRHPERLIGRSCLRPQGAPA
                     SAEEEMVRGWMQRLAEQG"
     gene            complement(5268..6305)
                     /locus_tag="VFF98_11090"
     CDS             complement(5268..6305)
                     /locus_tag="VFF98_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HZU64220.1"
                     /translation="MPSHTARPLRQRIIALYLLLGAANIAVWLWAYAVLRGHPALIGT
                     AFLAYSFGLRHAVDADHIAAIDNVTRKLMQEGQRPLGTGFFFAIGHSAVVLVVTLLIA
                     RTASAASGIEALKAAGGVISTCVSAFFLFAIAAMNLVILRSVYRSYRQVLAGGVYVEE
                     DLDMLLAGRGLMARLLRPLFALIRRSWHMAPLGFLFGLGFDTATEVGLMGLAAVQAAQ
                     GLSLGVVLLFPALFAAGMALVDTTDGIMMLGAYEWAFVKPIRKLYYNMTITLVAALVA
                     ILIGGIETLALIAHQWNLQGALWDGLRDLAGQFGLLGFAIVGLFALSWALSWLLYRRK
                     GLDHIEVIRAG"
     regulatory      complement(6495..6741)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(6797..7204)
                     /locus_tag="VFF98_11095"
     CDS             complement(6797..7204)
                     /locus_tag="VFF98_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU64221.1"
                     /translation="MRSSLAAVCAAALLAPALLAPMPALAGEGGAPDGEHIFHSRCQI
                     CHTVAANGKPGQAAPNLRGVVGRPAASTAFRGYSAALRASGLTWTPALIDTFLQGPAK
                     LVPGTKMAIILKDPAERAALVGWLAALSPPAGR"
     gene            complement(7226..8017)
                     /locus_tag="VFF98_11100"
     CDS             complement(7226..8017)
                     /locus_tag="VFF98_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64222.1"
                     /translation="MARPSACLFSALFFLAGLAPAAPALAAPEEIQVYTNEINDPGEI
                     GLELHANTVPTGNAAPDHAGGEGSTGRLRLTPEWSLGLGEHFELGAYVPLMTLDDHGA
                     FRADGFKVRLKWLGRHAERGVYYGLNYEIGREDIHLDQNAWNNEVKLIGGWEGDRWLI
                     GSNVNFDFALSGPAKGPPDVEWTTKLAYKLHEDTLIGVETYNGMGSTARFGQFGASDQ
                     STFATFDTRMGIWDLNLGIGRGYGSNPDHLILKMIIGLPIGHLHH"
     gene            complement(8242..8769)
                     /locus_tag="VFF98_11105"
     CDS             complement(8242..8769)
                     /locus_tag="VFF98_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HZU64223.1"
                     /translation="MTDTPPELYYLTQDIFVGDVIALGAAVSAGDWRPDYVVGIGRGG
                     LVPAVFLSHRLNLPMLSIDHSSKLPGFAGELLHKVAEKSAAGTRLLFVDDINDSGGTM
                     ATIRQILAEAGCDEANLRFAVLMDNIRSAVRVDYFAQTIDRATDKRWFVFPWEAMGMA
                     DTLTEEALSVPERLA"
     gene            complement(8909..9844)
                     /locus_tag="VFF98_11110"
     CDS             complement(8909..9844)
                     /locus_tag="VFF98_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZU64224.1"
                     /translation="MTLPPFTIALAQYPIEALADWPAYEAKLSRWVAQAAQGGAALAV
                     FPEYGAMELASLDPASMGDLAGSLGFVAGLAARVDALHARLAQAHGLYVLAASLPVQR
                     ADGAAYVNYVNRARLFAPSGAMAHQDKAVMTRFEAERWGISAGADGLALFDTALGRIG
                     IAICYDSEFPLIARAFAEAGAHLLLAPSCTDSAHGYWRVRIGCQARALEGQFYVAQSP
                     TVGDAPWSPAVDENRGAAGLFAPPDRHAGQGWAMPADGVVALGEMDAAQWLFAQVEPA
                     RVEALRADGGVLNTRDWARQPGAAPLPPVRVVALA"
     gene            complement(9844..10431)
                     /locus_tag="VFF98_11115"
     CDS             complement(9844..10431)
                     /locus_tag="VFF98_11115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU64225.1"
                     /translation="MSLSIRPFTGAEIHAALADLAALRIAVFAAFPYLYDGDPAYEAA
                     YLAGFADAPGAVLVAALDGARIVGAATAAPMWAQQAPFRAPFEAAGLDTQALFYFGES
                     VLLAEYRGQGIGHAFFDQREAQARAQGAHAATFASVIRAQDHPARPADYRPLDPFWRA
                     RGYAPVPGLVTHLAWKEWGEQAESPKPLQYWLKHF"
     gene            10507..10692
                     /locus_tag="VFF98_11120"
     CDS             10507..10692
                     /locus_tag="VFF98_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HZU64226.1"
                     /translation="MAEIINLRRARKARARTEAGRTAEANRALFGEATPLRKARAAEE
                     ARRLQALEGARLEKDEG"
     gene            10692..10907
                     /locus_tag="VFF98_11125"
     CDS             10692..10907
                     /locus_tag="VFF98_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64227.1"
                     /translation="MRTTYDAATVRLYHLDGAAEGGAAETLCYAPLAEALRVAAAQSA
                     EVQEGLFIATDNDVIAYLDLVDEGGAG"
     gene            complement(10926..11906)
                     /locus_tag="VFF98_11130"
     CDS             complement(10926..11906)
                     /locus_tag="VFF98_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZU64228.1"
                     /translation="MPITTGATLYAFTNEYLTRRYSFADLLREVAARGLGPGIEVIGF
                     QSIKTFPEVTDAFAENFRALVAELGLAPVSLGINSDRFLRPGQPVEDAHLLEYHKRQM
                     RSAHKLGFPILRYQFSTPPAMVADLAPYAEELGLKLGLEVHAPAHAHHPLVLAYREMY
                     AKVNSPALGWIPDFGGTASRIPPSLLEAARARGARESLITLLEEIWPGPGPSHEKAAT
                     LRAAALRDGHAPEHIQAVSLAFFILANGDPKSWAELADRTIHIHGKFYGVGADGREEA
                     VDYETILPIFRDAGVDATIVAEWEGHAYSTRDAFAEVEKFQAMCRRILAA"
     gene            complement(11917..12309)
                     /locus_tag="VFF98_11135"
     CDS             complement(11917..12309)
                     /locus_tag="VFF98_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6379 domain-containing protein"
                     /protein_id="HZU64229.1"
                     /translation="MTHTLLPVERVIGESGLAASADGLRLDMRLPWYRSLPLSTVRVE
                     ALSLDGAPVDLADALFELDGRRWPLAALADEIDSFWFVLDSAFLILPQLHPAPGSRHE
                     VALTLAVFPPYIPGMKRANSQTATLAVA"
     gene            complement(12328..13296)
                     /locus_tag="VFF98_11140"
     CDS             complement(12328..13296)
                     /locus_tag="VFF98_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU64230.1"
                     /translation="MRLATLRDGSPDGALAVISADGARYLAAPGLSLLAAIGDWGRAE
                     PVLQRLAAGVAAGQGGRLDPARLQAPLPRTWQWLDGSAFLQHGKLMQVAFHLPPIETD
                     RPLMYQGLSDRFYGPTDDIPLPSEADGIDFEGEYAVITDAVPMGTSAADAAGHIRLIV
                     QLNDWSLRAIAPVEMKTGFGWVQAKPACSMAPFAITPDELGAAWADGRVGADLHVAWN
                     GAAFGQPNGAEMSVSFADLVAHAARTRDLVAGTLIGSGTVSNAAYATVGSACISERRA
                     IEIIAAGQPATPFMGFGDAVRMEARLPDGRCPFGAIDQRVIRAVAP"
     gene            complement(13296..14093)
                     /locus_tag="VFF98_11145"
     CDS             complement(13296..14093)
                     /locus_tag="VFF98_11145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZU64231.1"
                     /translation="MARRFIDLSITLCNEVVSDPPFMRPEITYQTHSETVAELGHFFP
                     GVGAEQTPDGAGFAAAEWVRLTTHNGTHLDAPWHFHPTMDAHDGAPKRAITIDEVPLE
                     WCFQRGVKLDFRHLPDGHVVTAAEVEAELARIGHTLQPLDIVLVNTAAGMALGRPDFV
                     NVGCGMGYEATMYLTSRGVRVTGTDAWSWDAPFSYTAEKVKETGDTSLIWEGHKAGRD
                     IGYCHLEKLHNLEALPATGFTVSCFPAKIKGASAGWTRAVAIIEEEG"
     gene            complement(14112..>14311)
                     /locus_tag="VFF98_11150"
     CDS             complement(14112..>14311)
                     /locus_tag="VFF98_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64232.1"
                     /translation="AGLWPRALLLAAASALGALVLAAAALAATGAVLLPFAPALAAKL
                     AFGAALAAFITPLGLKAVLAP"
BASE COUNT         2490 a         4600 c         4939 g         2282 t
ORIGIN      
        1 cgagccggtg gcggcggttt cacggaagcc cttttcaatc gcggggatat attccttggg
       61 aatgttaccg cccttgatct cgtccttgaa gacgaagccc gaaccgcgct cacccgggct
      121 cagcttgacc ttcacgcggc caaactggcc ggtgccgccc gactgcttct tgtgggtgta
      181 gtcgatgtcc acgggcttgg cgagatattc gcggtaagcc acctgcggcg cgccgacatt
      241 ggcttccacc ttgaactcgc ggcgcatgcg gtcgacgatg atgtcgaggt gcagttcgcc
      301 catgcccttg atgatcgtct ggcccgattc gtggtcggtc gaaacgcgga acgagggatc
      361 ctcggccgac aggcgattga gcgcgatgcc catcttttcc tggtccgcct tggtcttggg
      421 ctccaccgac agctcgatca ccggctcggg gaattccatg cgctccagca cgatggggaa
      481 acgctcggcg cacagcgtgt caccggtggt ggtttccttc atgccggcca gcgcgatgat
      541 gtcgcccgcg aaggcctcat cgatgtcctt gcgctcgttc gcatgcattt ccagcatacg
      601 accgaccttt tccttcttgt ccttcaccga attcaggtag gtgcccttgg tgagcgtgcc
      661 cgaatagatg cggatgaagg tgagcgagcc gacgaaggga tcgttcatca ccttgaaggc
      721 cagagccgag aacggcgcgt catccttggc aggacggctg tccttctcgt cgctgtccat
      781 cttgacgccc tggacgtcag gaatgtcgag cggcgaaggc agataatcca ccacggcgtc
      841 gagcaggggc tgcacgccct tgttcttgaa cgccgagccg cagcacaccg gcacgaacga
      901 ctggttcagc gtgcccttgc ggatcagcgc cttcagcgtg tcgacgtcgg gctcggtgcc
      961 ttccagatag gcttccatcg cgtcatcgtc ctgctcaacg gcaagctcga tgaggttggc
     1021 gcggtattcg gcggcttcgt cggcgaggtc ggccgggatc tcctcgtaga agaattcggc
     1081 gcccagcgcc tcgtccttcc agatgatcgc gcggttgtgc accaggtcaa ccagcccctt
     1141 cagatcggct tcgaggccga tcggaatgta gagcacggcc ggcttcgcac ccaggcggtc
     1201 gatgatcgac tgcacgcagt acttgaagtt ggcgccggtg cggtccagct tgttgatgaa
     1261 gcacatccgg ggcacgccat acttgtcagc ctggcgccac acggtttccg actgcggctc
     1321 cacgccggcc acgccatcga agcaggccac ggcgccgtcg agcacgcgca gcgaacgttc
     1381 cacctcgatg gtgaagtcca cgtggcccgg ggtgtcgatg atgttgatgc ggtggtcgtt
     1441 ccagaagcag gtcgtggcgg ccgacgtgat cgtgatgccg cgctcctgct cctgctccat
     1501 ccagtccatc gtggcggcac cgtcgtgcac ttcgccgatc ttgtaggact tgccggtgta
     1561 gtaaaggata cgctcggtgg tcgtggtctt gccggcgtca atatgcgcca taataccgat
     1621 gttgcggtat ttctcgagcg gatggctgcg ggccatggga ggttccttgg gttcgggtgg
     1681 agcgcctttg caagcaaccc cgcccttagg ttcatttcta tgacagccgt gtgacggggc
     1741 cgccaaagcg cccccgcccc aagggtttta ccagcggtag tgcgagaagg cgcggttcgc
     1801 gtccgccatg cggtgcgtat cttcgcgctt cttcacggca ttgccgcggt tgttggccgc
     1861 atcgagcagc tcgcccgaaa ggcgggccga catggtggtt tcgctgcggt tgcgcgcagc
     1921 ggtgatcagc cagcggatgg ccagcgcctg ggcgcgctcg gggcgcactt cgacgggaac
     1981 ctgataggtg gcaccaccaa cgcggcggct gcgcacttcg atctgcggct tcacattgtt
     2041 cagcgcatcg tggaacagct gaacagggtc agccttcgca cgggtctgca tggtgtcgag
     2101 cgcaccatag acgatgctct cggcgacgga cttcttgccg tcgagcatca ggttgttcat
     2161 gaacttcgac agcacctgat caccaaactt cggatcaggc aggatttccc gcttctcggg
     2221 acgacgacga cgcgacatcg cttaattcct taaattctgt aagaccaacg caagaggcca
     2281 gatggcctta cttcggacgc ttggcgccgt acttcgagcg gctctgcttg cggttcttca
     2341 cgccctgggt atccagcacg ccgcgcagca cgtgatagcg cacgcccgga agatcgcgca
     2401 cacgcccgcc gcggatcagc accaccgagt gctcctgcag gttgtggcct tcgcccggga
     2461 tgtacgaaat gacttcgcgg ctgttggtca ggcggatctt ggccaccttg cgaagcgccg
     2521 agttcggctt cttcggggtc gtcgtataga cgcgggtgca gacgccgcgc ttctgcgggt
     2581 tctgctccat tgcagggacc ttgctcttgg ccttctgcag ttcgcggccc ttgcggacca
     2641 gctggttaat cgtaggcatg aagctctctt caccttggtt gaatgatacc atccgcacag
     2701 gtgaagaatg acaaacccgc atgagcgaat caccgtgagg aagcccccac gcgacagcca
     2761 atacgaaccg aaacgctcca cccgcaccat ccggcaccgg gttactttga cggattacac
     2821 caaggtttca gcaggttgcc cccgccaaca cccggcacgc cccggcaatg ttcagctctt
     2881 caatgcagca ggcaggcaag gccggccccc acatgaaccc gcgcgcttta ggcctgttgg
     2941 gccagtgcgt caagaatggc gggatttggg ggccgatcag atgaccctgg cctgacagat
     3001 tgcagatgcg atgatggcat tccccctttc gttagggtgg atgccatctc tgaagaggcc
     3061 cggcgcatcc cttcgacggt tcaactgcgg gcgaataatc tggacaccat gcccattcgc
     3121 ccatgcgaac agttgctctc tggcccgacc cgcgccagca gcaccagaca tcagcggatc
     3181 ccagtaaagg atcgccatgc gggcaccggc cgcgtcagac agggatttga tttcgccaat
     3241 ggcgttcatc accgcgccga cattggccgc gttgaacgca ccggcagtgg acccgggatc
     3301 agccgccaca ccgagcccaa agcgcggcaa ggcatagcgg aacaggattt ccgaaagccc
     3361 cgtggccggg cgatgcgatg gaaaggacgg gttcctgtca agcagtgacg catcgccgaa
     3421 agcctgatcc agatcctttt cgttcacctc gagcaacaca accctggccc cgaacacacc
     3481 atgcgccttg agccaggccg cttcattctg gaccgcccag ccgccggcgg cgacattgag
     3541 cacttctgct ggcaaaccgg cggtgcgcag acaagcaccc gtgagcatgg gataggtttg
     3601 ctcgtcgttg atctgggttc ccccattggc cacactgtcg ccaatggaaa gaacgcgcag
     3661 gcgatcggcg gaaggctggc gaggaagggc tggcccgcgc cctccaagat cattgattgt
     3721 ggttattttc ccgagcctgt tgacgcgctg gcccggcacc agttcatagc ccgacgcact
     3781 ggatcgatag atcagtggat tgccaaaccc catatgacgg gcgactgcct ctgcgcccac
     3841 aagggcaagg gcggcggcgg caatccccca taggattcgg cacggcacgg cacggcacgg
     3901 cacggcacgg cacggcacgg cacggcacgg cacggcacgg cacggcacgg caattttagc
     3961 atcatatcct cgattgcaat aggtggagaa ggattagtac gcgttatcta ttgagatcaa
     4021 ttgttaaatg ccctgttccc ggttgcgggt gacaggcaag gttagctatt gcagcggccg
     4081 tatcgctatg ctgcggcgac aagcagcgca gaaatttggg agcgcgaggg ggtaaccccc
     4141 tcgcatggtt tcttttcttg ccccccttcc ctttcgggaa agcgcgcggc gatccgtggg
     4201 ggcttgtgcc atacggcggc atgttcccat aatgttctgt cctacatgcc tgtctgtttg
     4261 ctagacctcg cctcccttgt cggtggaggt tgggtgatgg gtggcggtgt gcggtgcgcg
     4321 tcgggcaggc cggggcggcg gacgagccgg gtggcgtttt cgagcgaggc gccggaaggg
     4381 gatgatccgt tgctggggtt tgcaccgtat ctgcacaagc agcccaggcg caatgcgatc
     4441 acgccggatc ggcagcgggc cttcattgcc gcgctggcgg cgacggggat tgtgacgcag
     4501 gcggcacgca gcatcggggc cagcatggag gccttgtaca ggctgcgcca ccagccgggg
     4561 gcgcaggggt ttgcggcggc gtgggaagcg gcggtggacc gggggatggc gcggctggag
     4621 gattgcgcgc tggagcgggc gattgccggc gaggagcggg tgatgatccg gcgcggcgag
     4681 gtggtggccc gctggacgcg ctatgacacg ccattgctgg tatttttgct gcgcaaccgg
     4741 cggcggggcc ggtttggcca ggaggccttg ctgagccggg cggagtggga ggcggtgcgc
     4801 gaggcggaag aggcgggcga ggcagaggtg gtgctggagc ggttgaacgc caagctggat
     4861 gcgatgcggg cgcgcatggc ggcggccgcg gagacggcag cggcagagga agacgcgacg
     4921 ccgggcgaag agggggcggg cgaagagcca gcggatgatc aggcggcggg gccagcgggc
     4981 gggcgggcgc tggaagtgaa ggggctggcc gggatggagg cggcctgcgg ggcgcagccg
     5041 gcaggcggcg cggcgcggat gctgccgggc tttttgcggc agggggatgc gggggtgggg
     5101 atgccgcgca tcgacccggg gcggcatccg gagcggttga tcgggcgatc ctgcctgcgg
     5161 ccgcaggggg cgccggccag cgcggaggag gagatggtgc gaggctggat gcaacgcttg
     5221 gcggagcagg ggtgacgctc ccccctgccc ctgcctctgc ggcccgatca gccggcgcgg
     5281 atcacctcga tgtgatccag ccccttgcgg cggtagagca gccaggacag cgcccagctg
     5341 agcgcgaaca ggccgacgat ggcaaagccc agcaggccga actgcccggc cagatcgcgc
     5401 aggccatccc acagcgcgcc ctgcaggttc cactggtggg cgatcagcgc cagcgtctcg
     5461 atcccgccga tgaggatggc caccagcgcg gcgacgaggg tgatcgtcat gttatagtag
     5521 agcttgcgga tgggtttgac gaaagcccat tcataggcgc ccagcatcat gatgccgtcg
     5581 gtcgtatcca ccagggccat gccggcggcg aacagcgccg ggaacagcag caccaccccc
     5641 agcgacaggc cttgcgcggc ctggacggcg gccaggccca tcaaccccac ttcggtggcg
     5701 gtgtcgaagc ccaggccgaa caggaagccc agcggcgcca tgtgccagct gcgccggatc
     5761 agcgcgaaca gcgggcgcag caggcgcgcc atcagcccgc ggccggccag cagcatgtcg
     5821 agatcctcct ccacatagac gccgccggcc agcacctgcc gataggaacg atagaccgag
     5881 cgcaggatga cgaggttcat cgccgcaatg gcaaacagga agaaggccga aacgcaggtg
     5941 gaaatcactc cgccggccgc cttcagcgcc tctatgccgc tggccgcgct ggccgtgcgg
     6001 gcgatcagca aggtgacgac gagcaccacc gccgaatggc cgatggcaaa gaagaagccg
     6061 gtgcccagcg gccgctggcc ttcctgcatg agcttgcggg tgacattgtc gatcgcggcg
     6121 atgtgatcgg catcgaccgc gtggcgcagg ccgaaggaat aggcgaggaa ggccgtgccg
     6181 atcagcgccg gatggccgcg caacacggca taggcccaca gccagacggc gatattggct
     6241 gcccccagca gcagataaag ggcaatgatg cgctggcgca aaggccgcgc ggtgtgcgat
     6301 ggcatggcat tcaagtcccg ctggcggtgc tggcagaacg gggccgcgcc ggccgatcct
     6361 gccgcgcagg ttcgattccg cgcccggatg ccgcagcgcc ccgccaatgg ccgcgccagc
     6421 acccggcggt ggccccgcca tgactgccgc ccggacggac aaaatccgcg ccgcgcccat
     6481 cccctggaac acagcatgag cgacgacgcg ccggcaggtc tcctggcttg cgggtcaaca
     6541 cgcggtgcaa agccttcccg cgccctgccc gcttcggcat ccggcccagt ggctgccccc
     6601 tggcctgtgg ccggaggcgg tatgcagcgc gctcgtcgct tacagttgca gggacagccc
     6661 gggatttggc ccagccttgc ggccggccgc acccgcgttc cctttcaagc ccttgcgggc
     6721 accggcgcgt gggaggcagc cgtgaacggc tgcccgtggc agggatagac cagcggccgc
     6781 cccctgccaa gaccccctag cgccccgctg gcggagacaa cgcggccagc cagccaacga
     6841 gggcggcgcg ttcggccgga tccttgagga tgatcgccat tttggtgccg ggcaccagct
     6901 tggccggccc ttgcaggaag gtgtcgatca atgccggtgt ccaggtgagg ccggaggccc
     6961 tgagcgctgc ggaatagccg cgaaacgcgg tggaggcggc cgggcgcccc accacgccgc
     7021 gcagattggg ggcggcctgc cccggtttgc cattggccgc caccgtgtgg cagatctggc
     7081 agcgactgtg gaagatatgt tcgccatcag gggcgccccc ctcgcccgcc agcgccggca
     7141 tgggagccag cagcgcggga gccagcagcg cggcggcaca cacggcggca agactggagc
     7201 gcatggcggg gcctttcggg gtggatcagt ggtgcagatg gccgatcggc aggccgatga
     7261 tcatcttgag gatgaggtga tcggggttgc tgccatagcc gcggccgatg ccaaggttca
     7321 gatcccagat gcccatcctg gtgtcgaagg tggcgaaggt ggattgatcg ctggcgccaa
     7381 actggccgaa ccgggcggtg gagcccatgc cattataggt ttccacgccg atcagcgtgt
     7441 cttcatgcag cttgtaggcc agcttggtgg tccattccac atcgggcggg cccttggcgg
     7501 ggcctgaaag ggcgaaatcg aaattgacat tgctgccgat cagccagcga tcgccttccc
     7561 agccgccgat cagcttcacc tcattgttcc aggcgttctg gtcgaggtgg atgtcctcgc
     7621 ggccgatctc gtaattcagg ccatagtaga cgccgcgctc ggcatggcgg cccagccatt
     7681 tgaggcgcac cttgaagcca tcggcccgaa aggcgccatg atcgtccagc gtcatcagcg
     7741 gcacataggc gcccagctcg aaatgttcgc caaggcccag cgaccattcc ggcgtgaggc
     7801 gcaggcgccc ggtgctgccc tcgccgccgg catgatcggg cgcggcattg ccggtgggca
     7861 cggtgttggc gtgcagttcc aggccgatct cgccaggatc gttgatctcg ttggtgtaga
     7921 cctggatttc ctcgggcgcg gccagcgccg gcgcggccgg ggcaaggccg gccaggaaga
     7981 acagggcgct gaacagacag gcagaagggc gggccattgc ttgaaccaca caaagaggca
     8041 gacgccaaaa ggcagacaga gcgccgcctt acaaaggccc gcaggccctg tctatcggcg
     8101 tgctggccca gcggatcgat gaaggcgcac gggccagaag ggggccattt gcgccccgcc
     8161 cgcgggggcc agcggcggcg ccctgccaga tggcaatggc gcggcagggg ccaggccctg
     8221 ccccgcgccc cctgccccct gtcaggccag ccgttcaggc accgacagcg cttcctcggt
     8281 cagcgtgtcg gccatgccca tcgcttccca ggggaagacg aaccagcgct tgtccgtcgc
     8341 gcggtcgatc gtctgggcga aataatccac tctcacggcc gagcggatat tgtccatcag
     8401 cacggcaaag cgcaggttcg cctcgtcgca gccggcctcg gccaggatct ggcggatggt
     8461 ggccatggtg ccgccgctgt cgttgatgtc gtccacgaac agcaggcgag tgccggccgc
     8521 gcttttctcg gccaccttgt gcagcagttc gccggcaaag ccgggcagct tggaggaatg
     8581 gtcgatcgac agcatcggca ggttcagccg gtgcgagagg aacaccgccg gcaccaggcc
     8641 gccgcggcca atgcccacga cataatccgg ccgccagtcg cccgcgctga cagcagcgcc
     8701 cagggcgatg acatcgccaa cgaaaatatc ctgcgtgaga taatagagtt cgggaggggt
     8761 gtcggtcatc cgattgtccg gcaaaagcgc gcgaatctgg cgcgggaagg ctgaacggca
     8821 catcctagcc ggcgcggccg tttccggcaa tccgggtttg gccgggtttg gccgggtttg
     8881 ggcgtggtgt tttatggccg gccggcgcct aggccagggc caccacgcgc accgggggca
     8941 gcggggcagc gccgggctgg cgggcccaat cacgcgtgtt gagcacgcca ccatcggcgc
     9001 gcagggcctc cacccgggcc ggctccacct gtgcgaacag ccattgcgcg gcatccattt
     9061 cgcccagcgc caccacgcca tcggccggca tcgcccagcc ctgcccggca tggcgatcgg
     9121 gcggggcgaa gaggccggcc gcgccccggt tctcgtccac cgccggcgac cagggggcat
     9181 cgcccacggt cgggctttgc gccacataga actgcccttc cagcgcgcgg gcctggcagc
     9241 cgatgcgcac gcgccaatag ccatgggcgc tatccgtgca ggagggcgcc agcaggagat
     9301 gggcgccggc ctcggcgaag gcgcgggcga tgaggggaaa ttcgctgtcg tagcaaatgg
     9361 cgatgccgat gcggccgagc gcggtgtcga acagggccag gccatcggcg ccggcggaaa
     9421 tgccccagcg ttccgcctcg aagcgggtca tcaccgcctt gtcctgatgg gccatggcgc
     9481 ccgatggggc aaacaggcgc gcgcggttca catagttgac ataggcggcg ccatcggccc
     9541 gctgcaccgg caggctggcg gccagcacat aaagcccatg ggcctgcgcc agccgggcat
     9601 gcagcgcatc aacgcgcgcg gccagccccg ccacaaagcc cagcgatccg gccagatcgc
     9661 ccatgctggc aggatcgagc gaggccagtt ccatcgcccc atattcgggg aacaccgcca
     9721 gcgccgcgcc gccttgcgcc gcttgcgcca cccagcggga aagctttgcc tcataggccg
     9781 gccaatcggc cagcgcctcg atcggatatt gcgccagggc gatggtgaag gggggcagcg
     9841 tcatcagaaa tgtttcaacc aatattggag gggcttgggg ctttccgcct gctcgcccca
     9901 ttccttccac gccagatggg taacaaggcc gggcaccggg gcatagccgc gcgcgcgcca
     9961 gaagggatcg agcgggcgat aatctgccgg ccgggcgggg tggtcttgcg cccggatcac
    10021 ggaggcgaaa gtggcggcat gggcgccttg cgcgcgggcc tgtgcctcgc gctggtcgaa
    10081 gaaggcgtgg ccgatgccct ggccgcgata ttcggccaga agcacgcttt cgccgaaata
    10141 gaagagcgcc tgcgtgtcca gcccggcggc ctcgaagggg gcgcggaaag gtgcctgctg
    10201 ggcccacatc ggcgcggcgg tggccgcccc cacgatccgc gcgccatcca gcgctgccac
    10261 cagcaccgcc cccggcgcat cggcaaaacc ggcgagatag gccgcctcat aggccggatc
    10321 gccatcgtag agatagggga aggcggcaaa gacggcgatg cgcagcgccg ccagatcggc
    10381 aagcgcggcg tggatttcgg cgccggtgaa ggggcggatg gacaggctca tgcccatcgc
    10441 ttaccctgat tgcccgcgcc aggcgagagg cctggcgccc gatccgcgcc tgcatcaggg
    10501 aagcttatgg ccgaaatcat caacctgcgc cgcgcccgca aggcccgcgc ccgcacagag
    10561 gccggccgca ccgccgaggc caaccgcgcc ctgttcggcg aggccacccc cttgcgcaag
    10621 gcccgcgccg cagaagaagc ccgccggctg caggcgctgg aaggcgcgcg gctggaaaag
    10681 gacgaaggct gatgcgcacc acttacgatg cggcaacggt gaggctctat cacctcgatg
    10741 gcgcggccga agggggcgcg gccgaaacgc tgtgctatgc gccgctggcc gaagcgctgc
    10801 gggtggccgc cgcgcaaagc gccgaggtgc aggaaggcct gttcatcgcc accgacaatg
    10861 acgtgatcgc ctatctcgat ctggtggacg agggcggcgc cggctgacgc cccctccccg
    10921 gcccctcagg cggcgaggat gcgccggcac atggcctgga atttctccac ctcggcaaag
    10981 gcatcgcggg tggaataggc atggccctcc cattcggcga cgatagtggc atccacgccc
    11041 gcatcgcgga agatcggcag gatggtttcg taatccaccg cttcctcgcg gccatcggcg
    11101 cccacgccat agaatttgcc gtggatgtgg atggtgcgat cggccagttc ggcccagctt
    11161 ttcggatcgc cattggccag aatgaagaag gcgaggctga ccgcctggat atgctcgggc
    11221 gcgtggccat cgcgcagggc ggccgcgcgc agggtggcgg ccttttcatg cgaggggccg
    11281 gggccgggcc agatctcttc cagcagggtg atcaggcttt cccgggcgcc gcgcgcgcgc
    11341 gcggcctcca gcaggctggg cgggatgcgg ctggcggtgc cgccaaaatc ggggatccag
    11401 cccagcgccg gggaattcac cttcgcgtac atctcgcgat aggccagcac cagcggatgg
    11461 tgggcatggg caggcgcgtg cacttcaagc cccagcttca ggcccagctc ctcggcatag
    11521 ggggcaaggt ctgccaccat cgccggcggg gtggagaact gatagcgcag gatgggaaaa
    11581 cccagcttgt gcgcgctgcg catctggcgc ttgtgatatt ccagcagatg ggcatcctcc
    11641 accggctggc cgggcctgag gaagcgatcg gaattgatgc ccagcgagac cggcgccagg
    11701 cccagttctg ccaccagcgc gcggaagttt tcggcaaagg catcggtcac ttcggggaag
    11761 gtcttgatcg actggaagcc gatcacctcg atccccgggc caaggccgcg ggcggccact
    11821 tcgcgcagca gatcggcaaa gctgtagcgg cgggtgagat attcgttggt gaaggcgtag
    11881 agcgtggcgc cggtggtgat gggcatgggg tatccttcag gcaacggcaa gggtggcggt
    11941 ctggctgttg gcgcgcttca tgcccgggat atagggcggg aagacagcca gcgtgagcgc
    12001 cacttcatgg cggctgcccg gtgccgggtg cagctgcggc aggatcagga aggcgctgtc
    12061 gagcacgaac cagaagctgt cgatctcatc ggcaagggcg gccagcggcc agcggcggcc
    12121 atccagctcg aacagcgcat cggccagatc caccggcgcg ccatcaaggc tgagcgcttc
    12181 caccctgacg gtggacagcg gcagcgagcg ataccagggc aggcgcatat ccagccgcag
    12241 accatcggcg ctggcagcaa ggccgctttc accgatcacg cgttcaacag gcagcagggt
    12301 gtgggtcatg gcactctttt ttccggatca gggggcaacg gcgcggatca cgcgctggtc
    12361 gatggcgccg aaggggcagc ggccatcggg caggcgcgct tccatccgca cggcatcgcc
    12421 aaagcccatg aagggggtgg caggctggcc ggcggcgatg atctcgatcg cccggcgttc
    12481 ggaaatgcag gccgagccga ccgtggcata ggccgcgttc gacaccgtgc ccgagccgat
    12541 gagcgtgccg gcgaccagat cgcgcgtgcg ggcggcatgg gcgacgagat cggcaaagga
    12601 gacgctcatc tcggcgccat tgggctggcc aaaggcagcg ccgttccacg ccacatgcag
    12661 gtctgcgccc acgcggccat cggcccatgc ggcgcccagc tcgtcgggcg tgatggcgaa
    12721 gggggccatg gaacaggcgg gctttgcctg cacccagcca aagccggtct tcatctcgac
    12781 aggggcgatg gcgcgcagcg accagtcgtt cagctggacg atgaggcgga tgtggccggc
    12841 ggcatcggca gcgctggtgc ccatcggcac ggcatcggtg atgacggcat actcgccctc
    12901 gaaatcgatg ccatcagcct cggatggcag gggaatatcg tctgtcggcc catagaaccg
    12961 gtcggacagg ccctgataca tcagcgggcg gtcggtctcg atcggcggca ggtggaaggc
    13021 cacctgcatc agtttgccat gctggaggaa ggccgagcca tcgagccatt gccatgtgcg
    13081 cggcagcggc gcctgcagcc gggccggatc gagccggccg ccctgcccgg cggccacgcc
    13141 cgccgccagc cgttgcagca ccggctctgc cctgccccaa tcgccaatgg ccgccagcag
    13201 cgacaggccg ggcgctgcca gatagcgcgc gccatcggcc gaaatcaccg ccagcgcgcc
    13261 atcggggctg ccatcccgca aggtggcaag gcgcatcagc cttcctcctc gatgatcgcc
    13321 acggcccgcg tccagccggc agaggcgccc ttgatcttgg cggggaagca ggaaacggtg
    13381 aagccggtgg ccggcagcgc ttccagattg tgcagctttt caaggtggca ataaccgatg
    13441 tcacgcccgg ccttgtggcc ttcccagatc agcgaggtgt cgcccgtctc cttcaccttt
    13501 tccgccgtgt agctgaaggg cgcatcccag ctccaggcgt cggtgccggt cacgcgcacg
    13561 ccgcggctgg tgagatacat cgtcgcctca tagcccatgc cgcagccgac attgacgaaa
    13621 tccggccggc ccagcgccat gccggccgcc gtgttgacca gcacgatatc gagcggctgc
    13681 agcgtgtggc cgatgcgggc cagctctgcc tccacctcgg cggcggtgac gacatggcca
    13741 tcgggcaggt ggcgaaaatc cagcttcacg ccgcgctgga agcaccattc cagcggcact
    13801 tcatcgatgg tgatggcgcg tttgggagcg ccatcatggg catccatcgt cgggtggaag
    13861 tgccaggggg catcaagatg cgtgccgtta tgcgtggtca gccgcaccca ttcggccgcg
    13921 gcaaagccgg cgccatcggg ggtttgctcc gcccccacgc cggggaagaa atggcccagc
    13981 tcggccaccg tttcgctgtg ggtctgatag gtgatctcgg gccgcatgaa gggcggatcg
    14041 gataccacct cgttgcacag ggtgatcgac aggtcgatga aacggcgggc catggggctc
    14101 tctccagaat gtcagggcgc cagcacggct ttcaggccga gcggggtgat gaaggcggca
    14161 agggcagcgc caaaggccag cttggccgcc agcgccgggg caaagggcag aaggacagcg
    14221 cccgtggccg ccagcgccgc cgccgccagc accagcgctc ccagcgccga ggccgccgcc
    14281 agcagcagcg cgcgcggcca caggccggcg g
//
LOCUS       DASRYE010000220        21582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_224492,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000220 DASRYE010000000
VERSION     DASRYE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 21582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21582
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224492"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="VFF98_11155"
     CDS             <1..1826
                     /locus_tag="VFF98_11155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZU64233.1"
                     /translation="AEGHARLTAQRRAALEKLAGLRGTLRELTEAAGVSEAVLRGMVG
                     AGLMVAETVDLDRPYPAPDADHAAPALADGQKAAASQFVAAVAAGGGADGFAPFLLDG
                     VTGSGKTEVYFEGIAEAIRRGRQVLVLLPEIALTEHFLRRFEARFGVAPVLWHSSLSQ
                     AERRRAWRAAAEGRAAVVVGARSALFLPFAQLGLIVVDEAHEVSFKQDDGVRYNGRDV
                     AVIRARLEKIPVILASATPALESLALAEAGVYRKIDLPARFGGAQLPTVTIIDLTRDP
                     PERGRWIAPPLVEALEDRLARGEQSLLFLNRRGYAPLTLCRHCGHRFQCDSCSAWLVE
                     HRLSQRLACHHCGQEQPMPQRCPECGEADCLVACGPGVERIAEEVATLFPDARLAIAT
                     SDTLGSAEKVAEFVESAEQGMIDVIVGTQLVTKGYHFPELTLVGVIDADLGLEGGDLR
                     AGERSYQQIAQVAGRAGRAQKPGEVMIQTRHPTAPVIAALAGGGRDAFYAAETENRRH
                     AHAPPFGRWAAIIVSSEDEAEARDAARAIGATRPQQDDIAVIGPAPAPLSLLRGRYRY
                     RLLVNARRSARLQDALRAWLSALNLPQGVRVGIDIDPYSFV"
     gene            complement(1851..2930)
                     /locus_tag="VFF98_11160"
     CDS             complement(1851..2930)
                     /locus_tag="VFF98_11160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZU64234.1"
                     /translation="MPAALSARPGASPDGPAAPDVAPDDEARWQAVCARDRAGDGQFV
                     FAVATTYIYCRPSCPARRPARAHVRFYADGAAARAAGFRPCQRCRPDEAPRDAAALAH
                     ALALLAAAGPAPALADLAAHTGYSAGHIRRLVERATGLTPAAYGRALRRQRAEAALAD
                     GGRVIDAVYAAGYGAPSGFYAEARGAAMAPAARARGGAGQLIRWAEAATTLGPLLVAA
                     TQKGVCFLAFGAGKAELAARFPAAVLEEGGAGFAALVAKVVAAVEQPGSGEAIPLDVA
                     GTAFQEAVWQALSRIPPGETLSYAELAVAAGFPGAARAVGQANGANRVAVLIPCHRVV
                     RADGALGGYAWGEAIKAELLRRERK"
     gene            3327..3881
                     /locus_tag="VFF98_11165"
     CDS             3327..3881
                     /locus_tag="VFF98_11165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZU64235.1"
                     /translation="MENSAGITASLQGRYAAALYDLASEQGQVPGIEADLNAIEAALA
                     ASEDLAKLIRNPLVRRDAAAAAVAGVADVLGLGDLARKFLGVLAGNGRLSALPDIIRA
                     FAAIAAAARGEVTAEVTSAHPLSDEQLADLAARLGAREGKTVKIKAGVDADLLGGLVV
                     RIGSRQIDSSIRTRINSLAQAMKG"
     gene            3913..5442
                     /gene="atpA"
                     /locus_tag="VFF98_11170"
     CDS             3913..5442
                     /gene="atpA"
                     /locus_tag="VFF98_11170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZU64236.1"
                     /translation="MDIRAAEISKVIKDQIANFGTEAQVSEVGSVLSVGDGIARIHGL
                     DKVQAGEMVEFANGVQGMALNLEADNVGVVIFGSDSEIREGDTVKRTGTIVDVPTGKG
                     LLGRVVDALGNPIDGKGPIMYTERRRVEVKAPGIIPRKSVHEPVQTGLKAIDCLVPVG
                     RGQRELIIGDRQTGKTAVAIDTFINQKSVNAGDDEGKKLYCIYVAVGQKRSTVAQIVR
                     QLEENGAMEYSVVIAATASEPAPLQYLAPYTGAAIGEFFRDNGMHAVIVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDENGSGSLTALPIIETQAGDV
                     SAYIPTNVISITDGQIFLETGLFYQGIRPAINVGLSVSRVGSSAQTKAMKKVSGSIKL
                     ELAQYREMAAFAQFGSDLDASTQKLLNRGARLTELLKQPQFSPLGFEEQTCVIFAGTN
                     GYLDAIPTKQVTEYEAELLSFLRNSHADLLKLIRDTKDLGDEAKGKLKAALDTFAKQF
                     A"
     gene            5474..6349
                     /locus_tag="VFF98_11175"
     CDS             5474..6349
                     /locus_tag="VFF98_11175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZU64237.1"
                     /translation="MASLKELKGRINSVKSTQKITKAKQMVAAAKLRKAQSAAEAARP
                     YAQALTEVMGSLASRIVISESSPRLLAGTGKDQVHLLVVANSDRGLCGAFNSNIVKAA
                     RAKAAELEAQGKQVAFYLVGRKGRAVIRREYPKAIIGGFDTTSVREAGYSEAEAIAAE
                     LLALFDEGKFDVAHLFFSKFRSALLQEPTEQQIIPVPAPKAQVATGGAAVEYEPDEEE
                     ILSALLPRYLKTQIFGALLENAASEQGASMTAMDNATRNAGDLISKLTIQYNRSRQAA
                     ITTELIEIIAGAEAL"
     gene            6379..7818
                     /gene="atpD"
                     /locus_tag="VFF98_11180"
     CDS             6379..7818
                     /gene="atpD"
                     /locus_tag="VFF98_11180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZU64238.1"
                     /translation="MSTTSTGKIAQVIGAVVDVAFEGALPAILTALETDNNGNRLVLE
                     VAQHLGENTVRTIAMDSTDGLTRGQPVSNTGAQISVPVGPKTLGRILNVIGEPIDERG
                     PVGAEQTAPIHAKAPEFIDQSTEAAILVTGIKVIDLLAPYARGGKIGLFGGAGVGKTV
                     LIQELINNIAKGHGGVSVFAGVGERTREGNDLYHEFLDAGVIAKDADGNPTSDGSKVA
                     LVFGQMNEPPGARARVALSGLTMAEYFRDQEGQDVLFFVDNIFRFTQAGSEVSALLGR
                     IPSAVGYQPTLATDMGSLQERITSTTKGSITSVQAIYVPADDLTDPAPAASFAHLDAT
                     TTLNRSIAELGIYPAVDPLDSTSRVLEPRVVGAEHYETARRVQETLQKYKSLQDIIAI
                     LGMDELSEEDKLVVQRARKIQRFLSQPFHVAEVFTGMPGKFVQLEDTVKSFKAVVDGE
                     YDHLPEAAFYMVGGIDEAVAKAKKLAAEA"
     gene            7823..8080
                     /locus_tag="VFF98_11185"
     CDS             7823..8080
                     /locus_tag="VFF98_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZU64239.1"
                     /translation="MALHFELVTPARLVLSENVHMVVVPGSEGEFGVLEGHAPFMATV
                     RDGAVKVYKTENGQPQEIIVAGGFAEVTPKGLKLLAEHVEG"
     gene            8309..9805
                     /locus_tag="VFF98_11190"
     CDS             8309..9805
                     /locus_tag="VFF98_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZU64240.1"
                     /translation="MSSFGRRMGIGGGFGQAGRPSFGVARPMRGPDMAGAQPDPAPGE
                     HTPEPGDSAAKSDAMARLADRMNATADAGRVTEGFEASVHKIKEQVLPRLLERIDPEA
                     AATLTKDELAEEFRPIIMEVLSELKITLNRREQFALEKVLIDELLGFGPLEELLGDPD
                     VSDIMVNGPDQTYIEKKGKLQLSTIRFRDEQHLFQIAQRIVNQVGRRVDQTTPLADAR
                     LKDGSRVNVIVPPLSLRGTAISIRKFSEKPITLDMLRDFGSMDDRMCTALKIAGACRM
                     NIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQ
                     GAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLG
                     RMENMILMGDIKIPKEAISRQIAESVDIIVQVKRLRDGSRRTTNITEVIGMEGDVIVT
                     QELFKFEYLDENEEGKIIGEFRPSGLRPYTLEKARQFGFDQAYLEACL"
     gene            complement(9824..10033)
                     /locus_tag="VFF98_11195"
     CDS             complement(9824..10033)
                     /locus_tag="VFF98_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64241.1"
                     /translation="MGWGWAQICALSGAGAAALALGAWWADRRHMRRRQLDRVGLVVW
                     RDVAFWATVAALLAWGAALRLWAQG"
     gene            complement(10044..11603)
                     /gene="gcvPB"
                     /locus_tag="VFF98_11200"
     CDS             complement(10044..11603)
                     /gene="gcvPB"
                     /locus_tag="VFF98_11200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZU64242.1"
                     /translation="MNAPNASGWKPHMGTAEAATAATASGDRALNLEEALIFELAGPG
                     GCGVDMEAPALPPVAGLERFLRQAPAALPGLSEPEVVRHYTRLSRQNYAIDLGPFPLG
                     SCTMKHNPRLNEKVARLPGFADIHPLQPKHTVPGALEAIAQLADWLIALTGMAAVAMT
                     PKAGAHGELCGLLCIRAALDARGENREVVLVPESAHGTNPATAAFAGFRVENIPATAD
                     GRVDLAALTARLGPDIAGVMITNPNTCGLFERDMKAISDAVHGVGGFVYCDGANFNAI
                     VGKVRPGDLGVDAMHINLHKTFSTPHGGGGPGSGPVVLSAALAPFAPLPFAQRGADGA
                     VHLIEEEEAPADHPASFGRMAAFHGQMGMFTRALAYILSHGADGLRQVAEDAVLNANY
                     VLRSLEDVLDAPFAAAGPCMHEALFSDNGLPEGFSTLDIAKGLIDEGFHPMTVYFPLV
                     VHGAMLVEPTETESKAGLDRFIASLRSLATRARAGDETLQSAPHFAPRRRVDETLAAR
                     KPRLVWQADAA"
     gene            complement(11600..12958)
                     /gene="gcvPA"
                     /locus_tag="VFF98_11205"
     CDS             complement(11600..12958)
                     /gene="gcvPA"
                     /locus_tag="VFF98_11205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZU64243.1"
                     /translation="MRYLPLTPADRAAMLARIGADDIDALFADVPATALLAGPVAGLA
                     PHASEMAVERHMARLAGQNLTAGAVPFFLGAGAYRHHIPASVDHLIQRGEFLTAYTPY
                     QPEIAQGTLQVLFEFQTQVARLFGTDIANASMYDGSTACWEAIAMAGRVTRRKKAVLA
                     AGLHPHYVSTAQTMARFTGDAILAATPLLAADAGDAATIAAIDGETAAVVVQYPDILG
                     RIPDLAAIAEAAHARGALLVAVVTEPVALGLIEAPGHLGADIVVGEGQSLGVGLNFGG
                     PYLGLFGARAKFVRQMPGRLCGETVDAEGKRAFVLTLSTREQHIRREKATSNICTNAG
                     LCALAFSIHMSLLGGEGLARLAALNHARARATAEALAAIPGVEVVNDAYVHEFAVRLP
                     TEARSAVRAMADRGVLGGVALGRLYPGAAGLENGLVVAASELTSDEDIAALVAAIKGV
                     IA"
     gene            complement(12966..13337)
                     /gene="gcvH"
                     /locus_tag="VFF98_11210"
     CDS             complement(12966..13337)
                     /gene="gcvH"
                     /locus_tag="VFF98_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZU64244.1"
                     /translation="MTRYFTADHEWIELDGAHATVGITDYAQEALGDITFVELPDAGR
                     ALAKGDSVAVVESVKAASDVYAPLAGTVTEVNGALEDEPELVNTSAELEGWLWRMDVA
                     EGADLAALMDEAAYKAHIAGL"
     gene            complement(13337..14539)
                     /gene="gcvT"
                     /locus_tag="VFF98_11215"
     CDS             complement(13337..14539)
                     /gene="gcvT"
                     /locus_tag="VFF98_11215"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZU64245.1"
                     /translation="MSETDLPETDILDGDDAPEVLSLPLEGWHVAKGARMVEFAGYRM
                     PVQYEGIIAEHLWTRAHAGLFDVSHMGQLLVSAPESDVATAAAALEALLPADLAALGL
                     GRQRYSLLLDEAGGILDDLMATRWREGFYLVVNGATKWDDIAHLREHLPDEITLTHLE
                     EQALLALQGPEAVMALGALGLQPGFAGIVPVEELGFMQAAPYLWGEVALGISRSGYTG
                     EDGFEISVPAGMAQALADALCAHPSVRPIGLGARDSLRLEAGLPLYGHDLDETTDPVS
                     AGLAFAISKRRRAEGGFMGAARVQQALADGTETIRVGLALDGRQAAREGAAVLADGVA
                     VGRVTSGGFAPSLGHPVAMAYVAAAHAAPGTVLSIEVRGRQLPARVVALPFVPNRYFR
                     KTAQNGAK"
     regulatory      complement(14572..14688)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(14689..14796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(14880..16073)
                     /locus_tag="VFF98_11220"
     CDS             complement(14880..16073)
                     /locus_tag="VFF98_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZU64246.1"
                     /translation="MPLSPLACHAAASRGREFAIEGAVARGPRTAYQRDRDRIIHSIA
                     FRRLRSKTQVFIAPDGDHYRVRLTHSLEVAQIGRVIARVLGLDEDLTEALCLAHDIGH
                     PPFGHSGEDALAAALAPHGGWDHNAHALRVLMRLESPYCEHDGLNLSWELLEGLAKHN
                     GPVGLGTEALGGRGRAANWALAELDAAFPLHLREWCGLEAQVAALADDIAYDNHDIDD
                     GLRAGFLTLDELMAQPFVADQWKQVEARHPHAPRERQLRELVRSQIGLMVNDLIAETW
                     KRAAGMESLADVRAAGRATVAFSPAVAEAERSLKRFMYERLYYHPEQLATAARAREVT
                     AALYAAYEADPALMGPGWAARLPADNPARARHIADFIAGMTDHFAISRYAAIFGHVPE
                     GLSNV"
     gene            complement(16143..16451)
                     /locus_tag="VFF98_11225"
     CDS             complement(16143..16451)
                     /locus_tag="VFF98_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64247.1"
                     /translation="MRRALFAVLIAPLALAGCGEGVARHRIEAALEQRGIAQGPARCM
                     ADRLVDRLSIAQLRKLEAVKAHAGDPVAAVLALQAIDDPEVVRVTITAAGLCLGGLDR
                     "
     gene            complement(16504..17919)
                     /locus_tag="VFF98_11230"
     CDS             complement(16504..17919)
                     /locus_tag="VFF98_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZU64248.1"
                     /translation="MEASAPNPWVALAYLVAGVLFILALRGLSSPATSRAGNRYGMIG
                     MTIAMVTTLLTHEIASLPEIAAALAIGGGVGFFTARRIKMTDMPQLVAAFHSLVGMAA
                     VLVGWAAYLNPAAFGIMEGAEIAAHSRIEMGLGIAIGAITFSGSVIAFLKLAGKMSGA
                     PILLPGRHVLNLGTLLAILGLVAWFTQDESLWVIAIVSALSFAIGFLLIIPIGGADMP
                     VVVSMLNSYSGWAAAAMGFTLHNTAMIITGALVGSSGAILSYIMCRAMNRGFLSVIAG
                     GFGADTGGSSAGGAPKEQRPWKRGSAEDAAFLLKEAENVIIIPGYGMAVAQAQHVLRE
                     MGDLLKKAGVNVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINAEFAQADVAF
                     IIGANDVVNPAAKTDKSSPIYGMPVFDVDKAKTVMFIKRSMGGVGYSGVDNDVFYMDQ
                     TMMLLADAKKMVEDIVKALAH"
     gene            complement(17919..18203)
                     /locus_tag="VFF98_11235"
     CDS             complement(17919..18203)
                     /locus_tag="VFF98_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HZU64249.1"
                     /translation="MDFVSILSIFVMACFVGYYVVWSVTPALHTPLMAVTNAISSVIV
                     VGALIAAAAAGSVVSKWLGLGAVVLASVNIFGGFAVTERMLAMYKKKEKK"
     gene            complement(18220..19341)
                     /locus_tag="VFF98_11240"
     CDS             complement(18220..19341)
                     /locus_tag="VFF98_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZU64250.1"
                     /translation="MKIAVLAERAMGETRVSASPETVKKFSALGALVAVEAGAGLAAS
                     IADADYAAAGAQLGDAGAVVAGADIVLGVQGPDVALLAGAKPGAWIAAGLDPFGQRAR
                     VDAYAAAGFEALAMEFMPRITRAQSMDILSSQSNLAGYKAVIDAAATYGRAFPMMMTA
                     AGTITAAKAFIMGVGVAGLQAIATARRLGAQVSATDVRSATKEQILSLGAKPIFVESV
                     AGIEGEGSGGYATEMSAEYQKAQAELVSAHIARQDIVVTTALIPGRAAPRLISDAQIA
                     SMKPGSVIFDLAAPQGGNVEGCVADEVVVRHGVKIIGYANTPARLPADASALFARNLY
                     NFLSAFWDKEAGRPVLDEEIGNAIRLTQGGAVVNPRLLG"
     gene            complement(19363..19488)
                     /locus_tag="VFF98_11245"
     CDS             complement(19363..19488)
                     /locus_tag="VFF98_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HZU64251.1"
                     /translation="MATTHDIEAARATYEGFLRLFRIGTPIVALIAALVLFLLTR"
     gene            19701..21122
                     /locus_tag="VFF98_11250"
     CDS             19701..21122
                     /locus_tag="VFF98_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU64252.1"
                     /translation="MADTDTRLLMLIDDEPAQSRLITALAAREGWRTLVVRDAETAIA
                     TLGTRQGMQLGAIILDQWVPGDDACALIAELRARRPALPVLMLTTSASPLLAVEAMRA
                     GATDYLIKPVAPERLMLALRSATTRETPRDELQPLTEKMPGPPDFEAMIGAAPAFRSA
                     LAIAAKAARGHAPVLVEGESGTGKEMLVRAIHAASQRAKLPLRIVNAGSTPANSIESA
                     IFGHEKGAFPGAFERQIGALQHCDGATLVIDEIDRLPLSVQARLAEALARGDCRPIGA
                     RHSFRVDVRLIAASNYPLKELVATGDFLPELYEALSATTVAVPPLRERAGDIAALSRH
                     FLARIGEQPGLRQLGITDGALALLAAYSWPGNVRQLQAVLFRAAVFCDGDALTAEDFP
                     QLSSLIGTQGSFAASQTHESAGITLYTPDGNLRPLEEIEADVIRLAIGHYRGRMTEVA
                     RRLGIGRSTLYRKLSDLGIDNAA"
     gene            complement(21162..>21582)
                     /locus_tag="VFF98_11255"
     CDS             complement(21162..>21582)
                     /locus_tag="VFF98_11255"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZU64253.1"
                     /translation="LHADGTYADVLLDVFDWLKARRDAALAAGIRAENLLLDPGIGFG
                     KTLAENLALMNGLELFHALGCPLLVGASRKRIVGALSNEAPAHLRLGGSLALALRMMQ
                     AGVQIIRVHDVAETVQALRVWRGLRDQALTDFAQVVA"
BASE COUNT         3668 a         7565 c         7075 g         3274 t
ORIGIN      
        1 cggcagaggg ccacgcccgc ctcaccgccc agcgccgggc cgcgctggag aagctggcgg
       61 gcctgcgcgg cacgctgcgc gagctgaccg aggcggccgg cgtttccgaa gcggtgctgc
      121 gcggcatggt gggggccggg ctgatggtgg ccgagacggt ggacctcgac cggccctacc
      181 ccgcgcccga tgccgaccat gccgcccctg ccctcgccga tggccaaaag gccgccgcca
      241 gccagtttgt ggcggcggtg gcggcaggcg gcggggcgga tggctttgcc cccttcctcc
      301 ttgatggcgt caccggctcg ggcaagacgg aggtctattt cgaggggatt gccgaggcga
      361 tccggcgcgg gcgacaggtg ctggtgctgc tgcccgaaat tgccctgaca gagcatttcc
      421 tgcgccgttt cgaggcgcgc ttcggggtgg cgccggtgct gtggcattcc tccctgtcgc
      481 aggcagagcg gcggcgggcg tggcgggcgg cggccgaggg gcgggcagcg gtggtggtgg
      541 gcgcacgctc ggcgctgttc ctgccctttg cgcagctggg cctcatcgtg gtggacgagg
      601 cgcatgaagt ctccttcaag caggatgacg gcgtgcgcta taatggccgc gacgtggcgg
      661 tgatccgcgc gcggctggag aagatcccgg tgatcctcgc cagtgccacc ccggcgctgg
      721 aaagcctggc gctggccgag gccggggtct atcgcaagat cgacctgccg gcgcgctttg
      781 gcggcgccca gctgcccacc gtcaccatca tcgacctcac gcgtgacccg cccgagcgcg
      841 gccgctggat cgccccgccg ctggtggaag cgctggaaga ccggctggcg cgcggcgagc
      901 aatcgctgct gttcctcaac cggcgcggct atgccccgct gaccctgtgc cgccactgcg
      961 gccaccgctt ccagtgcgac agttgcagcg cctggctggt ggagcatcgc ctgtcgcagc
     1021 ggctggcctg ccaccattgc gggcaggaac agcccatgcc ccagcgctgc ccggaatgcg
     1081 gcgaggccga ttgcctggtg gcctgcggcc cgggcgtgga gcgcatcgcc gaggaagtgg
     1141 ccacgctttt ccccgatgcg cgcctggcca tcgccacgtc cgacacgctg ggcagcgccg
     1201 aaaaggtggc cgaattcgtc gagagcgccg aacagggcat gatcgacgtg atcgtcggca
     1261 cgcagctggt caccaagggc tatcacttcc cggagctgac gctggtgggg gtgatcgacg
     1321 ctgatctggg cctcgaaggc ggcgacctgc gcgcgggcga gcggtcctat cagcagatcg
     1381 cccaggtggc cggccgcgcc ggccgcgccc agaagcccgg cgaggtgatg atccagaccc
     1441 gccaccccac agcgccggtg atcgccgccc ttgccggcgg cgggcgcgat gccttctatg
     1501 ccgccgagac ggaaaaccgc cgccatgccc atgccccgcc ctttggccgc tgggcggcga
     1561 tcatcgtctc ttccgaagac gaggccgagg cgcgcgatgc cgcccgcgcc attggcgcca
     1621 cccggcccca gcaggacgac attgccgtga tcggcccggc gccggcgccg ctgtcgctgc
     1681 tgcgcgggcg ctatcgctat cggctgctgg tgaacgcccg gcgcagcgcc cgcctgcagg
     1741 atgccctgcg cgcctggctc tcggcactca acctgccgca gggcgtgcgc gtggggatag
     1801 acatcgatcc ctattcgttt gtgtgacagg gcggcgccgg cccgctccct ttatttccgc
     1861 tcgcgccgca gcagttcggc cttgatcgcc tcgccccagg catagccgcc cagcgcgcca
     1921 tcggcccgca ccacccggtg gcaggggatc aacaccgcca cccggttggc gccattggcc
     1981 tgccccaccg cccgcgccgc gccgggaaag ccggcggcca cggccagctc cgcatagctc
     2041 agcgtttcgc ccggggggat gcggctgaga gcctgccaca ccgcttcctg aaacgccgtg
     2101 ccggccacgt cgagcgggat cgcctcgccg ctgccgggct gttccaccgc ggccaccacc
     2161 ttggccacca gggcggcaaa gcctgcgccc ccctcctcca gcacggcggc ggggaagcgg
     2221 gcggccagct ctgccttgcc ggcgccaaaa gcgaggaagc acacgccctt ttgcgtggcc
     2281 gccaccagca gcggccccag cgtggtggca gcctctgccc agcggatcag ctgccccgcc
     2341 ccgccgcgcg cgcgcgcggc cggcgccatc gctgccccgc gcgcctcggc ataaaagccc
     2401 gagggcgcgc catagcctgc cgcatagacg gcatcgatca cccgcccgcc atcggccagc
     2461 gccgcttctg cccgctggcg ccgcagggca cggccatagg cggccggggt caggccggtg
     2521 gcgcgttcca ccagccggcg gatgtggccc gcgctatagc cggtgtgggc ggccagatca
     2581 gccagcgccg gcgccggccc agccgctgcc agcaacgcca gcgcgtgggc cagggctgcg
     2641 gcatcgcgcg gggcctcgtc gggccggcag cgctggcagg gccggaaccc cgccgcccgc
     2701 gccgccgcgc catcggcata gaaacgcaca tgcgcgcgcg cgggccgccg cgccgggcag
     2761 gacgggcggc aatatatata ggtggtggcg acagcgaaga cgaattgccc atcgcccgcg
     2821 cgatcacgcg cgcaaaccgc ctgccagcgc gcctcgtcat ctggggcaac atctggggcg
     2881 gcggggccat cggggctggc gccgggccgt gcggaaaggg cggcgggcat catcatcgaa
     2941 tctccgactt tagtatgaac cccggattta ccggtatttc cgcccggttt tgccacagga
     3001 aacgggcccg cgcgtcctga aaaatggctt caaaattttt gcagaacggc ccatctgcgg
     3061 cagaaaaccg gggaaattca cagcgctgcg gcctatcagg ggtgcccccg cccgccccct
     3121 tgtggccaca tcgccccatc agcccccctt gccggctgcc cactgccgct ttaacccccc
     3181 gacttgcatc agctgccaat cgtcgctatg cgcgcccccg tctgagggcg gccgcattgg
     3241 tatgtaattg cggcgcaaga ccagtcagcg ttccggcatg gcaccacccg gtgtccgtgc
     3301 catccggtaa gaggggaccg accagcgtgg agaattccgc cggcattacc gccagtttac
     3361 aaggccgcta tgcagcggcg ctgtatgatc tggccagcga gcagggccaa gtccccggca
     3421 tcgaggcgga tctgaacgcc atcgaggcgg cgctggctgc ttctgaagat ctggccaaac
     3481 tgattcgcaa cccgctcgtg cgccgcgatg cggccgctgc tgcggtggca ggcgtggccg
     3541 atgtgctggg ccttggcgat ctggcccgca agtttctggg cgtgctggcc ggcaacggcc
     3601 gcctgtcggc cctgcccgac atcatccgcg cctttgccgc catcgcggct gccgcccgcg
     3661 gcgaagtgac cgccgaggtg accagcgccc acccgctgag cgacgagcag ctggccgatc
     3721 tggccgcccg cctcggcgcc cgcgaaggca agaccgtgaa gatcaaggcg ggcgtcgatg
     3781 ccgatctgct ggggggcctg gtggtccgca tcggcagccg gcagatcgac agctcgatcc
     3841 gcacccgtat caattccctc gcccaggcga tgaagggcta agcccttaaa acggcttgat
     3901 gaaaggctga acatggacat tcgcgccgca gaaatctcga aggtcatcaa ggaccagatc
     3961 gccaatttcg gcaccgaggc ccaggtctcg gaagtcggct ccgtgctgtc ggtgggcgac
     4021 ggtatcgccc gcatccacgg cctcgacaag gtgcaggccg gcgagatggt ggaattcgcc
     4081 aatggcgtgc agggcatggc cctcaacctc gaggccgata acgtcggcgt cgtgatcttc
     4141 ggctcggaca gcgagatccg cgaaggtgac acggtgaagc gcaccggcac catcgtcgac
     4201 gtgccgaccg gcaagggcct gctgggccgc gtggttgacg cgctgggcaa cccgatcgac
     4261 ggcaagggcc cgatcatgta taccgagcgc cgccgcgtgg aagtgaaggc gccgggcatc
     4321 atcccgcgca agagcgtgca cgagccggtg cagaccggcc tcaaggcgat cgactgcctg
     4381 gtgcccgtgg gccgtggcca gcgcgagctg atcatcggtg accgccagac cggcaagacc
     4441 gccgttgcca tcgacacctt catcaaccag aagtcggtga atgcaggcga tgacgagggc
     4501 aagaagctct actgcatcta cgtggccgtg ggccagaagc gctccaccgt ggcgcagatc
     4561 gtgcgccagc tggaagagaa cggcgccatg gaatattccg tggtgatcgc cgccaccgct
     4621 tcggagccgg ctccgctgca gtatctggcg ccctacaccg gcgctgccat cggcgagttc
     4681 ttccgcgaca acggcatgca tgccgtgatc gtgtatgacg atctttccaa gcaggccgtg
     4741 gcctatcgtc agatgtcgct gctgctgcgc cgcccgccgg gccgcgaagc ctaccccggc
     4801 gacgtgttct acctgcacag ccgcctgctg gagcgtgcag ccaagctgaa cgacgagaac
     4861 ggctcgggct cgctgaccgc gctgccgatc atcgagaccc aggcgggcga cgtgtcggcc
     4921 tacatcccga ccaacgtgat ctcgatcacc gacggccaga tcttcctgga aaccggcctg
     4981 ttctaccagg gcatccgccc ggccatcaac gtcggtctgt cggtgagccg cgtgggctcc
     5041 tcggcccaga ccaaggcgat gaagaaggtg tcgggctcga tcaagctgga gctggcccag
     5101 tatcgcgaaa tggccgcctt cgcccagttt ggctcggacc tcgatgcctc gacccagaag
     5161 ctgctgaacc gcggtgcgcg cctgaccgag ctgctcaagc agccccagtt ctcgccgctc
     5221 ggctttgaag agcagacctg cgtgatcttc gccggcacca acggctacct cgatgccatc
     5281 cccaccaagc aggtgacgga gtatgaggcc gagctgctga gcttcctgcg caacagccac
     5341 gccgacctcc tcaagctgat ccgcgacacc aaggatctgg gcgacgaggc caagggcaag
     5401 ctgaaggcag cgctggatac gttcgccaag cagttcgcct gagccccgcc attacctaag
     5461 gaggtagttg gcaatggcca gccttaagga actcaagggc cggatcaact cggtcaagtc
     5521 gacccagaag atcaccaagg ccaagcaaat ggtggccgcc gcgaagctgc gcaaggcgca
     5581 gtccgcggcg gaggccgcgc gcccctatgc ccaggccctt accgaggtca tgggcagcct
     5641 cgcctcgcgg atcgtgatca gcgagagcag ccccaggctg ctggccggca ccggcaagga
     5701 tcaggttcac ctgctcgttg tcgccaattc cgaccgcggc ctgtgcggtg ctttcaactc
     5761 gaacatcgtc aaggccgcac gcgccaaggc ggccgagctg gaagcccagg gcaagcaggt
     5821 ggcgttctat ctcgtcggcc gcaaggggcg cgcggtgatc cgccgcgaat accccaaggc
     5881 gatcatcggc ggcttcgaca ccacctcggt gcgcgaggcc ggctatagcg aggccgaagc
     5941 gattgccgcc gagctgctcg ccctgttcga cgagggcaag ttcgacgtgg cgcatctgtt
     6001 cttcagcaag ttccgcagcg ccctgctgca ggagccgacc gagcagcaga tcatcccggt
     6061 gcccgcgccc aaggcgcaag tggccaccgg cggcgcggcg gttgaatatg agccggacga
     6121 ggaggaaatc ctctcggccc tgctgccgcg ctacctcaag acccagatct tcggcgcgct
     6181 gctggagaat gcggcttccg aacagggcgc ctcgatgacc gcgatggaca atgccacgcg
     6241 caacgccggc gacctcatca gcaagctcac catccagtac aatcgcagcc gccaggccgc
     6301 gatcacgacc gaactcatcg aaatcatcgc tggcgccgaa gcgctctgac gacgagacga
     6361 aaaggcaagg aacaacccat gtccaccacc tccaccggca agattgccca agtcatcggc
     6421 gccgtcgtcg acgttgcttt cgaaggcgca ctgcccgcga ttctcaccgc gctcgagacc
     6481 gacaacaacg gcaaccgcct ggttctcgaa gttgcccagc acctgggcga gaacaccgtg
     6541 cgcaccatcg ccatggattc gaccgacggc ctgacccgcg gccagcccgt gtcgaacacc
     6601 ggcgcgcaga tctcggtgcc ggttggcccc aagacactgg gccgcatcct caacgtgatc
     6661 ggcgagccga tcgacgagcg cggccccgtg ggcgccgagc agaccgcccc gatccacgcc
     6721 aaggcgccgg aattcatcga ccagtcgacc gaagcggcga tcctggtcac cggcatcaag
     6781 gtgatcgacc tgctcgcgcc ctatgcgcgc ggcggcaaga tcggcctgtt cggcggcgct
     6841 ggcgtgggca agaccgtgct catccaggaa ctgatcaaca acatcgccaa gggccacggc
     6901 ggcgtgtcgg tgttcgctgg cgtgggtgag cgcacccgcg agggcaacga cctctaccac
     6961 gaattcctcg acgccggcgt tatcgccaag gacgccgatg gcaatccgac ctcggatggc
     7021 tcgaaggtgg ccctcgtgtt cggccagatg aacgagccgc cgggcgcccg tgcccgcgtc
     7081 gccctctcgg gcctgacgat ggccgaatac ttccgcgacc aggaaggcca ggacgtgctg
     7141 ttcttcgtgg acaacatctt ccgcttcacc caggctggtt cggaagtgtc ggcactgctg
     7201 ggccgcattc cctcggccgt gggctatcag ccgacgctgg ccaccgacat gggctcgctg
     7261 caggagcgca tcacctccac caccaagggt tcgatcacct cggtgcaggc gatttacgtg
     7321 ccggccgacg acttgaccga cccggcgccg gccgcctcgt tcgcgcactt ggacgcgacg
     7381 accacgctga accgctcgat cgccgagctg ggcatctacc cggccgttga cccgctcgac
     7441 tcgaccagcc gcgtgctgga gccgcgcgtg gtgggtgccg agcactacga gaccgctcgc
     7501 cgcgttcagg aaacgctgca gaagtacaag tcgctgcagg acatcatcgc gatcctgggc
     7561 atggacgaac tgagcgagga agataagctg gtcgtgcagc gcgcccgcaa gatccagcgc
     7621 ttcctgtcgc agcccttcca cgtggccgaa gtgttcaccg gcatgcccgg caagttcgtg
     7681 cagctggaag acacggtgaa gtcgttcaag gccgtcgtcg atggcgaata cgaccacctg
     7741 cccgaagcgg ccttctacat ggttggcggc atcgacgagg cggtcgcgaa ggccaagaag
     7801 ctggccgcgg aggcctgagc ccatggcact gcactttgaa ctcgtaacgc cggcccggct
     7861 tgtcctttcg gagaacgtcc acatggtggt cgtgcccggc agcgagggcg agttcggcgt
     7921 gctggaaggc catgccccgt tcatggccac cgtccgcgat ggcgctgtca aggtctacaa
     7981 gaccgaaaac ggccagccgc aggaaatcat cgtggcaggc ggttttgccg aagtgacgcc
     8041 caaggggctg aagcttttgg cggagcatgt ggagggctga acgcccccac gcagctacgc
     8101 gcaaacacga aaggccggcg gggcaacctg ccggcctttt gctgtgcggg cccggcaggc
     8161 ccggcgggca ggcccctcgc cttctgccgc gcctcaccgt ctcattagca aatcctaagg
     8221 gtttccgcct tattcaccat gcctctaagc agcatggtag gcatgcagcg cgaaggaaca
     8281 aacggttgat taagggaagg cttctggcat gagctcgttc gggcgtcgca tgggaattgg
     8341 cgggggcttt ggccaggctg gtcgccccag ttttggcgtg gcgcgcccca tgcgcgggcc
     8401 cgacatggcc ggtgcccagc ccgatcccgc gccgggcgaa cacacgcccg agcctggcga
     8461 cagcgccgcc aaatccgatg ccatggcgcg cctggccgac cggatgaacg ccaccgccga
     8521 tgccggccgc gtgaccgagg gcttcgaggc cagcgtccac aagatcaagg agcaggtgct
     8581 gccacgcctg ctggaacgca tcgaccccga ggccgccgcc accctcacca aggacgagct
     8641 ggccgaggaa ttccgcccga tcatcatgga ggtgctctcg gagctgaaga tcaccctcaa
     8701 ccggcgcgag cagttcgcgc tggaaaaggt gttgatcgac gagctgctgg gctttggccc
     8761 gctggaagag ctgctgggcg acccggatgt ctcggacatc atggtcaacg ggcccgacca
     8821 gacctatatc gagaagaagg gcaagctgca gctttccacc atccgcttcc gcgacgagca
     8881 gcacctgttc cagatcgccc agcgcatcgt caaccaggtg ggccgccggg tggaccagac
     8941 gacgccgctg gccgacgcgc gtctgaagga tggtagccgc gtcaacgtca tcgtgccgcc
     9001 gctgagcttg cggggcacgg cgatctcgat ccgcaaattc tccgaaaagc cgatcacgct
     9061 ggatatgctg cgcgatttcg gctcgatgga cgatcgcatg tgcaccgcgc tgaagatcgc
     9121 cggggcctgc cggatgaaca tcgtcatctc gggcggcacg ggttcgggca agacgacgat
     9181 gctgaacgcc ctttccaaga tgatcgaccc gggcgagcgc gtgctgacga tcgaggatgc
     9241 ggcggaactg cgccttcagc agccccactg gctgccgctg gaaacccgcc cgcccaatct
     9301 ggaaggccag ggcgcgatca ccattggcga cctcgtcaag aacgccctgc gcatgcggcc
     9361 cgatcgcatc atcctgggcg aaatccgcgg cgcggaatgt ttcgacctgc tggcggcgat
     9421 gaacaccggc catgatggct cgatgtgcac gctgcacgcc aattccccgc gcgaatgcct
     9481 ggggcgcatg gaaaacatga tcctgatggg cgacatcaag atccccaagg aagcgatcag
     9541 ccggcagatt gccgaatcgg tcgatatcat cgtgcaggtg aagcgcctgc gcgatggcag
     9601 ccgccgcacc accaacatca ccgaggtgat cggcatggag ggcgatgtga tcgtgacgca
     9661 ggaattgttc aagttcgaat atctcgacga aaacgaggag ggcaagatca tcggcgagtt
     9721 ccgcccctcc ggcctcaggc cctacacgct ggaaaaggcc cgccagttcg gctttgatca
     9781 ggcctatctg gaagcctgcc tgtaaaagcc cgcctccagg cgctcagccc tgggcccaca
     9841 ggcgcagggc cgccccccag gccagcagcg ccgccaccgt ggcccagaag gccacatcgc
     9901 gccagaccac cagccccacc ctgtcgagct ggcggcggcg catgtggcgc ctgtctgccc
     9961 accacgcccc gagcgccagc gctgcagcgc cagcgcccga tagcgcgcag atctgcgccc
    10021 atccccatcc catacccagg cgcctaggcg gcgtctgcct gccacacgag gcggggcttg
    10081 cgggcggcca gcgtttcatc cacccggcgg cggggggcga aatgcggggc cgattgcagc
    10141 gtctcgtcac cggcacgggc gcgggtggcc aggctgcgca ggctggcgat gaagcggtcg
    10201 agcccggcct tgctctcggt ctcggtcggc tccaccagca tggcgccatg caccaccagc
    10261 gggaaataga ccgtcatcgg gtggaagccc tcgtcgatca gccccttggc gatgtcgagg
    10321 gtggagaaac cctcgggcag gccgttgtcg ctgaacagcg cctcgtgcat gcaggggccg
    10381 gccgcggcaa agggcgcgtc cagcacgtct tccaggctgc gcagcacgta attggcgttc
    10441 agcacggcat cctcggccac ctgccgcagg ccatcggcgc catggctgag gatataggcc
    10501 agcgcgcggg tgaacatgcc catctgcccg tggaaggcgg ccatgcggcc aaagctggcg
    10561 ggatgatcgg ccggcgcttc ctcctcctcg atgaggtgca ccgcgccatc tgcgccgcgc
    10621 tgggcgaagg gcagcggcgc aaaaggcgcc agcgccgccg acagcaccac cgggccagag
    10681 cccggcccgc cgccgccatg gggcgtggag aaggttttgt gcaggttgat gtgcatggca
    10741 tccacgccca gatcccccgg gcgcaccttg ccgacgatgg cgttgaaatt ggcgccatcg
    10801 caatagacga agccgcccac cccatgcacc gcatccgaga tcgccttcat gtcgcgctcg
    10861 aacaggccgc aggtgttggg gttggtgatc atcaccccgg cgatatcagg gcccagccgg
    10921 gcggtgagcg cggccagatc cacccggcca tcagccgtgg cagggatgtt ttccacccgg
    10981 aagccggcaa aggcggcggt ggccgggttg gtgccatggg cgctttcggg caccagcacc
    11041 acctcgcggt tttcaccgcg cgcatcaagc gcggcgcgga tgcacagcag gccgcacaat
    11101 tcgccatggg cgccggcctt gggggtcatg gcaacggcgg ccatgccggt cagcgcgatc
    11161 agccagtcgg ccagctgggc gatggcctcc agcgcgccgg gcacggtgtg cttgggctgc
    11221 agcgggtgga tatcggcaaa gccgggcagg cgcgccacct tctcgttgag gcgcggattg
    11281 tgcttcatgg tgcagctgcc cagcgggaac ggcccgaggt cgatcgcgta attctggcgg
    11341 ctgaggcggg tgtagtggcg caccacttcg ggttcggaga ggcccggcag ggcagcgggc
    11401 gcctggcgca ggaagcgttc cagcccggcc accggcggca gcgccggcgc ttccatgtcc
    11461 acgccgcagc caccagggcc ggccagctcg aagatcagcg cctcttccag gttcagcgcc
    11521 ctgtcgccac tggcggtggc ggcggtggcg gcttcggcgg tgcccatatg cggcttccag
    11581 cccgatgcgt tgggcgcgtt catgcgataa ctcccttgat ggcggccacc agcgcggcaa
    11641 tgtcctcgtc gctggtcaat tcgctggcgg ccacgacaag gccgttctcc agcccggccg
    11701 cgcccggata aagccggccc agcgccacgc cccccagcac gcccctgtcg gccatggcgc
    11761 gcacggcgct gcgcgcttcg gttggcaggc gcacggcaaa ttcatgcaca taggcatcgt
    11821 tcaccacctc cacgccgggg atcgcggcca gcgcctcggc ggtggcgcgg gcgcgggcgt
    11881 ggttcagcgc ggcaagccgg gccagcccct cgccgcccag caggctcatg tggatggaaa
    11941 aggccagcgc gcacaggccg gcattggtgc agatgttgct ggtggccttt tcgcggcgga
    12001 tatgctgctc gcgggtagac agcgtcagca cgaaggcgcg cttgccctcg gcatccaccg
    12061 tctcgccgca caggcggccg ggcatctgcc gcacgaattt ggcgcgggcg ccgaacaggc
    12121 ccagataggg cccgccgaaa ttgagcccca cgcccagcga ctgcccctcg cccaccacga
    12181 tatcggcgcc cagatggccc ggcgcctcga tcagccccag cgccaccggc tctgtcacca
    12241 ccgccaccag cagcgcgccc cgggcatggg cagcctcggc aatggcggcc agatcaggga
    12301 tgcggccgag gatatcggga tattgcacca ccacggccgc cgtctcgcca tcgatcgcgg
    12361 cgatggtggc ggcatcgccg gcatcggctg ccaacagggg cgttgcagcc agaatggcat
    12421 cgccggtgaa gcgcgccatc gtctgcgcgg tggagacata atgcgggtgc aacccggctg
    12481 ccagcaccgc cttcttgcgc cgggtgacgc ggccggccat ggcaatcgcc tcccagcaag
    12541 cggtcgagcc gtcatacatc gaggcattgg cgatatcggt gccgaacagg cgggccacct
    12601 gcgtctggaa ttcgaacagc acctgcagcg tgccctgggc gatttccggc tgatagggcg
    12661 tgtaggcggt gaggaattcg ccgcgctgga tcaggtgatc aacgctggcg ggaatatggt
    12721 ggcgataggc ccccgccccc aggaagaagg gcaccgcgcc cgctgtcagg ttctgcccgg
    12781 ccaggcgcgc catgtgccgc tccaccgcca tttcgctggc atggggggca aggccggcca
    12841 ccggccctgc cagcagcgct gtggccggca catcggcaaa cagcgcatcg atatcatcgg
    12901 ccccgatccg cgccagcatg gcggcgcggt cggccggggt aagcggcaaa tagcgcatgt
    12961 ctgtcttaca gtcccgcgat atgggccttg taggccgcct catccatcag cgcggccaga
    13021 tccgcgccct cggccacatc catgcgccac agccagcctt ccagctcggc cgaggtgttg
    13081 acgagctcgg gctcgtcttc cagcgcgccg ttgacttccg tcaccgtgcc ggcgagcggc
    13141 gcatagacgt cggaagccgc cttcacgctt tccaccacgg ccacgctatc gcccttggcc
    13201 agcgcccggc cggcatcggg cagttccaca aaggtgatat cgcccagcgc ttcctgcgcg
    13261 taatcggtaa tccccaccgt ggcgtgggcg ccgtccagct cgatccattc atggtctgcg
    13321 gtgaaatagc gggtcatcac ttggctccgt tctgggcggt tttgcggaaa tagcggttgg
    13381 gcacgaaggg cagcgccacc acgcgggcag gcagctggcg cccgcgcacc tcgatggaaa
    13441 gcacggtgcc gggcgcggca tgggcggcgg ccacataggc catggcaaca ggatgcccca
    13501 ggctgggcgc aaagcccccc gaggtgacgc ggcccaccgc cacgccatcg gccagcaccg
    13561 cggcgccctc gcgcgcggcc tggcggccat ccagcgccag ccccacgcgg atggtctcgg
    13621 tgccatcggc cagcgcctgc tgcacgcgcg ccgcgcccat gaagccgcct tcggcccggc
    13681 gccgcttgct gatggcaaag gcaaggccgg cgctcaccgg gtcggtcgtc tcgtccagat
    13741 catggccata gagcggcagg cccgcttcca gccgcagcga atcgcgcgcg ccaaggccga
    13801 tgggcctgac cgagggatgg gcgcaaaggg catcggccag cgcctgggcc atgccggcgg
    13861 gcaccgagat ctcgaacccg tcctcacccg tatagcccga gcgcgagatg cccagcgcca
    13921 cttcgcccca aagataaggc gcggcctgca tgaagcccag ttcctccacc ggcacgatcc
    13981 cggcaaagcc cggctgcagc cccagcgccc ccagcgccat caccgcttcc ggcccctgca
    14041 gcgccagcag cgcctgctct tccagatggg taagggtgat ctcgtcgggc agatgctcgc
    14101 gcaggtgggc gatgtcgtcc cacttggtgg cgccgttgac gacgagatag aacccctcgc
    14161 gccagcgcgt ggccatcagg tcgtcgagaa tgccgcccgc ctcgtccagc agcagcgaat
    14221 agcgctggcg gccaaggccg agcgcagcca gatccgccgg cagcagcgct tccagcgcgg
    14281 cggcggccgt ggccacatcg ctctcgggcg cgctcaccag cagctggccc atgtggctca
    14341 catcgaacag gcccgcatgg gcgcgcgtcc acagatgttc ggcgatgatg ccttcatatt
    14401 ggaccggcat gcgatagccg gcaaattcga ccatccgcgc gcccttcgcc acatgccagc
    14461 cttccagcgg cagcgagagg acctcgggcg cgtcgtcgcc atcaaggata tcggtttcag
    14521 gcagatctgt ttcgctcacg cggctttcct catgacacaa gcacccatca cggcgccccc
    14581 tctgtcacgg aaaacctgag agcttggccc gctggcatca aagccttgcg gggttacccc
    14641 ttcggtggcc cggccccggg gggcagagcg ctttccagag cgtcgctatc gatccacacg
    14701 gtccttttgc ctgagagatt ccggggcggt tgctccttcg gcgccctgtg gccggccttg
    14761 catggcctgc cgcagggtct ctcccgcatg tttcagcgac tggcccgcaa atcctatgcc
    14821 gcgccgcaaa agtcaaaggc caattcaaaa aaatttgtgg ggccgatcaa aaaaactgat
    14881 cacacattgc taagcccttc cggcacatgg ccaaaaatgg cggcatagcg cgatatggcg
    14941 aaatgatccg tcatgccggc gatgaagtcg gcgatgtggc gggcgcgggc ggggttgtcg
    15001 gcgggcaggc gggcggccca gcccgggccc atcagcgccg ggtccgcctc ataggcggca
    15061 tagagggcag cggtcacctc gcgcgcgcgc gcggcggtgg ccagctgctc ggggtggtaa
    15121 tacagcctct cgtacatgaa gcgcttgagg ctgcgctcgg cctcggccac ggccggcgag
    15181 aaggccaccg tggcccgccc tgccgcgcgc acatcggcca ggctttccat gccggcggcg
    15241 cgcttccatg tctcggcgat aaggtcgttc accatcaggc cgatctggct gcgcaccagc
    15301 tcgcgcaatt gccgctcgcg cggggcgtga gggtggcgcg cttccacctg cttccactga
    15361 tcggccacaa agggctgcgc catcagctcg tccagcgtga ggaagccggc gcgcaggcca
    15421 tcgtcgatat cgtgattgtc gtaggcgatg tcgtcggcca gcgccgccac ctgcgcttcc
    15481 agcccgcacc attcgcgcag atgcagcgga aacgccgcat ccagctcggc cagcgcccag
    15541 ttggccgccc tgccccgccc gcccagcgcc tctgtgccca gccccaccgg gccattgtgc
    15601 ttggccagcc cttccagcaa ttcccagctg aggttgaggc catcatgctc gcaatagggg
    15661 ctttccaggc gcatcagcac gcgcagcgca tgggcgttgt gatcccaccc gccatggggc
    15721 gccagcgcgg ccgccagcgc atcctcgccg gaatggccga agggcgggtg gccgatgtca
    15781 tgcgctaggc acagcgcctc ggtcaggtcc tcgtccagcc ccagcacgcg ggcgatgacg
    15841 cggccgatct gcgccacttc caggctgtgg gtgaggcgca cgcggtaatg atcgccatcg
    15901 ggcgcgatga acacctgcgt cttggagcgc aggcggcgaa aggcgatgga atggatgatg
    15961 cggtcgcggt cgcgctgata ggcggtgcgc gggccacggg ccaccgcgcc ctcgatggcg
    16021 aattcgcgcc cccggctggc ggcggcatgg caggcaagcg gcgaaagcgg catcaaaaat
    16081 ccattccgtc tggggctttg cgcaagggtt agccgagcgc tgccgcccgc gccagcccaa
    16141 gcctagcgat ccagcccgcc aaggcacagg cccgccgcgg tgatggtcac gcgcaccact
    16201 tcgggatcat cgatcgcctg cagcgccagc acagcggcga cgggatcacc cgcatgggcc
    16261 ttcaccgctt ccagcttgcg caactgggca atggagagcc tgtccaccag ccggtcggcc
    16321 atgcagcggg cggggccctg ggcaatgccg cgttgctcca gcgcggcctc gatgcggtgg
    16381 cgggccaccc cctcgccgca gccggccagg gccagagggg cgatcagaac ggcaaaaagg
    16441 gcacggcgca tgggcttcct ttcggttgcc gctgcgccgc gcccctttca aaaggcagga
    16501 atatcagtgg gccagcgcct tcacgatatc ctccaccatc ttcttggcgt cggccagcag
    16561 catcatcgtc tggtccatgt agaacacatc attatcgacg cctgaatagc ccacgccgcc
    16621 catgctgcgt ttgatgaaca tcaccgtctt ggccttgtcc acgtcgaaca ccggcatgcc
    16681 gtagatcggg ctggacttgt cggtcttggc ggcagggttc accacgtcgt tggcgccgat
    16741 gatgaaggcc acatcggcct gggcgaattc ggcgttgatg tcctccagct cgaacacctc
    16801 gtcatagggc acgttggctt cggccagcag cacgttcatg tggcccggca tgcggcccgc
    16861 caccggatgg atggcgtatt tcacattcac gcctgccttc ttcagcaggt cgcccatttc
    16921 gcgcagcacg tgctgggcct gcgccacggc catgccatag ccggggatga tgatgacatt
    16981 ttccgcttcc ttcagcagga aggcggcatc ctcggcgctg ccgcgcttcc acgggcgctg
    17041 ttccttgggc gcgccgccgg cgctgctgcc accggtgtcg gcgccaaagc cgccggcgat
    17101 caccgagagg aagccgcggt tcatcgcccg gcacatgata taggagagga tcgcgcccga
    17161 gctgcccacc agcgcgccgg tgatgatcat cgccgtgttg tgcagggtga agcccatggc
    17221 cgccgccgcc cagcccgaat aggaattgag catggagacg accaccggca tatccgcccc
    17281 gccgatgggg atgatgagca ggaagccgat ggcaaaactg agcgccgaga cgatggcgat
    17341 cacccacagg ctttcatcct gggtgaacca ggccaccagc cccaggatcg ccagcagcgt
    17401 gcccaggttc agcacatggc ggccgggcag caggatgggc gcgccgctca tcttgccggc
    17461 cagcttgagg aaggcgatga ccgagcccga gaaggtgatc gcgccgatgg caatgcccag
    17521 ccccatctcg atgcggctgt gcgcggcgat ctcggcgcct tccatgatgc cgaaagcggc
    17581 cgggttgaga taggccgccc agcccaccag caccgcggcc atgcccacca gcgagtggaa
    17641 ggcagccacc agctggggca tgtcggtcat cttgatgcgc cgcgcggtga aaaagcccac
    17701 gccgccgcca atggccagcg ccgccgcgat ctcgggcagg ctggcgatct cgtgggtcaa
    17761 gagcgtggtc accatggcga tggtcatgcc gatcatgcca tagcggttgc ccgcgcgcga
    17821 tgtggccggg ctggaaaggc cgcgcagggc cagaatgaac agcacgccgg ccaccagata
    17881 ggccagcgcc acccacggat tgggagcaga agcttccatc acttcttctc cttcttcttg
    17941 tacatcgcca gcatgcgctc ggtcacggca aagccgccga agatgttgac cgaggccagc
    18001 accaccgcgc ccagccccag ccacttgctg accacgctgc cagcggcggc cgcggcaatc
    18061 agcgcgccca ccacgataac cgaggaaatg gcattggtga cggccatcag cggcgtgtgc
    18121 aacgccggcg tgaccgacca gacaacgtaa tagccgacaa agcaggccat cacgaagatc
    18181 gagagaatag aaacgaaatc catgcgttca gccccccatt cagcccagca gccgcggatt
    18241 gacgacagcc ccgccctgcg tcaggcggat ggcattgccg atctcctcgt ccagcacggg
    18301 ccggccggcc tccttgtccc agaaggcgga gagaaaatta tacaggttgc gcgcgaacag
    18361 cgccgaggca tcggccggca ggcgcgcggg cgtgttggca tagccgatga tcttcacgcc
    18421 atggcgcacc accacctcat cggccacaca cccctcgaca ttgccgccct ggggcgcggc
    18481 cagatcgaag atgacagagc ccggcttcat gctggcgatc tgcgcatcgg aaatgaggcg
    18541 cggcgccgcc cggcccggga tcagcgcggt ggtgacaacg atatcctgcc gggcgatatg
    18601 ggcagacacc agctcggcct gggccttctg gtattccgcg ctcatctcgg tggcatagcc
    18661 gccgctgccc tcgccctcga tgccggccac gctttccacg aagatcggct tggcgcccag
    18721 gctgaggatc tgctccttcg tggccgaacg cacatcggtg gcagacacct gcgcgcccag
    18781 ccggcgcgcc gtggcaatcg cctgcagccc ggccacgccc acgcccatga tgaaggcctt
    18841 ggcagcggtg atcgtgccgg ccgcggtcat catcatcggg aaggcgcggc cataggtggc
    18901 cgccgcatcg atcaccgcct tgtagccggc caggttcgat tgcgaggaga ggatatccat
    18961 cgactgggcg cgggtgatgc gcggcatgaa ctccatcgcc agcgcctcga acccggcagc
    19021 ggcataggca tccacccgcg ctcgctggcc gaaaggatca agcccggcgg caatccacgc
    19081 ccccggtttt gcccctgcca gcagcgccac atccggcccc tgcacgccca gcacgatatc
    19141 cgcacccgcc accacagcgc cggcatcgcc aagctgggcg ccggcggcgg cataatcggc
    19201 atcggcaatg ctggcggcaa ggccggcgcc cgcttccacc gccaccaaag cacccagcgc
    19261 actgaatttt ttcaccgtct cggggctcgc cgagacgcgc gtttccccca tcgcccgttc
    19321 agccagaacg gcgattttca cttgtcctgt catgcttgtt cctcagcggg tcagcagaaa
    19381 gagcaccagc gccgcgatca acgccacaat cggcgtgccg atcctgaaca ggcgcagaaa
    19441 accttcgtat gttgcccgcg ccgcttcaat gtcgtgtgtc gttgccatcg cgcttgtgat
    19501 ccctaccctg ggccgtatcg cggcccccgt tatacgcccc ttgttcacgg cctttgccgg
    19561 ccgatgccgc cggtgtaccc cccgcctccg cccggctcaa ggggcgaagc atccgtcatg
    19621 ttcatttggt taggtttaag ctcctcttta ctcgcgcgcg cctatccctt cacttcaccc
    19681 aagatggacc ttcggggaag atggccgaca ccgacacccg cctgctgatg ctgatcgacg
    19741 atgagccggc gcaaagccgc ctgatcaccg cgcttgccgc gcgcgagggc tggcgcacgc
    19801 tggtggtgcg cgatgccgaa accgccattg ccacgctggg cacgcgccag ggcatgcagc
    19861 tgggggcgat catcctcgac cagtgggtgc cgggcgatga tgcctgcgcg ctgatcgccg
    19921 aattgcgcgc ccgccgcccg gccctgccgg tgctgatgct gaccaccagc gcctcgccgc
    19981 tgctggcggt ggaagcgatg cgggcgggcg ccaccgatta tctcatcaag ccggtggcgc
    20041 cagagcggct gatgctggcc ctgcgcagcg ccaccacccg ggaaaccccg cgcgacgaat
    20101 tgcagccgct gaccgagaaa atgcccggcc cgcccgattt cgaggcgatg atcggcgccg
    20161 cgcccgcctt tcgcagcgct ctggcgattg ccgccaaggc cgcgcgcggc catgccccgg
    20221 tgctggtgga aggcgaaagc ggcaccggca aggaaatgct ggtgcgcgcc atccatgccg
    20281 ccagccagcg cgccaagctg ccgctgcgca tcgtcaacgc cggatcgacc ccggccaatt
    20341 ccatcgaatc agccatcttc ggccatgaga aaggcgcctt tcccggcgct ttcgaacggc
    20401 agatcggcgc cctgcagcat tgcgatggcg ccaccctggt gatcgacgag atcgaccgcc
    20461 tgccgctatc ggtgcaggcg cggctggcag aagcgctggc ccgcggcgat tgccggccga
    20521 tcggcgcgcg ccattccttc cgcgtggatg tgcggctgat cgcggccagc aactatccgc
    20581 tgaaggaact ggtggccacc ggcgatttcc tgcccgaact gtatgaggca ctgagcgcca
    20641 ccaccgtggc ggtgccgccc ttgcgggaac gggcgggcga tatcgctgcc ctctcgcgcc
    20701 atttcctcgc ccgcatcggc gagcagccgg gcctcaggca actgggcatc accgatggcg
    20761 cgctggcgct gctggcggcc tacagctggc cgggcaatgt gcgccagttg caggcggtgc
    20821 tgtttcgcgc ggcggtgttc tgcgatggcg atgcgctgac ggcggaggat ttcccccagc
    20881 tgtccagcct gatcggcacg cagggcagct ttgccgccag ccagacccac gaaagcgccg
    20941 gcatcaccct ctacacgccc gatggcaacc tgcgcccgct ggaagagatc gaggccgatg
    21001 tgatccgcct ggccatcggc cactatcgcg ggcggatgac ggaagtggcc cgccggctgg
    21061 gcatcggccg ctccaccctc tatcgcaagc tcagcgatct cgggatcgat aacgcagcct
    21121 gaagcggggc agcctgaagc ggggcggcct gaagcggccc ctcaggccac cacctgggca
    21181 aaatcggtca gcgcctgatc gcgcaggccc cgccacaccc gcagcgcctg cacggtttcg
    21241 gccacatcat gcacgcggat gatctgcacg cccgcctgca tcatgcgcag cgccagcgcc
    21301 agcgatccgc ccagccgcaa atgcgccggc gcctcgttcg acagcgcgcc gacgatccgc
    21361 ttgcggctgg cccccaccaa cagcgggcag cccagcgcgt ggaacagctc cagcccgttc
    21421 atcagcgcca gattttccgc cagcgtcttg ccaaagccga tgcccggatc aagcagcagg
    21481 ttttccgccc tgatgcccgc cgccagcgcc gcatcgcgcc ttgccttcag ccagtcgaac
    21541 acatcgagca gcacatcggc ataggtgcca tcggcatgca gg
//
LOCUS       DASRYE010000221        12197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_84166,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000221 DASRYE010000000
VERSION     DASRYE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84166"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFF98_11260"
     CDS             complement(<1..1383)
                     /locus_tag="VFF98_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU64254.1"
                     /translation="MTLLRSVAMAAVSAIAIANAGLAHAAGTDTASTDAADDNGVILV
                     TAQHRSENLQNVPLAVSVVKGETLEDYHANGADTLLSLSGKVPSLYVESTTGRIFPRF
                     YIRGLGNTDYYLGASQPVSIIQDDVVEEHVVLKSTPAFDIGQVEVLKGPQGSLFGRNT
                     TAGIIKFESAKPTEYMTGDTSISWGSHNSMNVESGVGGPLSANHVLKFRLSSLYQQRS
                     GWISNTYAGISDDGTKAGRNVMGGFKEGDVRLQLQLDPEPATSINLSTHYRNYSGSAT
                     IFYRGSIQKGTSRPAAGFNYGAVQYDEGNNNPQAYTTWGQSLHVRHDFGGVSLTSITA
                     YENTSGYSRGDTDGGAGVNNGNVGFSESMGRVAQLNQWSQELRLANSDKARLKWQLGG
                     LYFDSRDDTEFYQRAYFLTSNALGGTPNPNNWVMLHNINTSWAVFGQASYKITDKLTI
                     SAGARETNDTK"
     gene            1662..2222
                     /locus_tag="VFF98_11265"
     CDS             1662..2222
                     /locus_tag="VFF98_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64255.1"
                     /translation="MPALPARRPLAALAAALCAGAGLLAAPPAIARDGLGVFGNWAAF
                     RDGAVPRCYAIALAEPERAGLRGGNRQAGLPRQFDAYADVGSWPRRGVRGQLHLRASR
                     RLGAGVAQLSLGGQRIALTSGQADLWAADPRADAAIMAAMRQGGRMQLLARDASGHLF
                     TDSWQLDGAACAMDAATLACAADARR"
     gene            complement(2219..2635)
                     /locus_tag="VFF98_11270"
     CDS             complement(2219..2635)
                     /locus_tag="VFF98_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64256.1"
                     /translation="MGWKAGVAGVGVAALAVVVAVPLVRGHGAACAAVAGQADGALAE
                     AVSARALAGAAPASAQAGGAPDLGQLAGLLGADPGDSGAAAVQAWLARAPLAGGRCVG
                     LWWRYALLAQVAPGRAHAEWLALDMAALDGAALEAR"
     gene            2694..3986
                     /gene="rlmN"
                     /locus_tag="VFF98_11275"
     CDS             2694..3986
                     /gene="rlmN"
                     /locus_tag="VFF98_11275"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZU64257.1"
                     /translation="MSDTTASAPVAHPAAHMPIPGAIDPVPTPREVTPRADGRVDLTG
                     LPCAEITRLLGEAGLDTKAAKLRAKQLFHWIYHRGVTDFDGMTDIAKTMRPWLAERFV
                     IGRPEIVVSQHSTDGTRKWLLRTADGHEFEMVFIPDADRGTLCVSSQVGCTLNCRFCH
                     TGTMRLVRNLTPGEIVGQVMLARDALGEWPKAGQADFSELGDDLVDDNGGYSADGRLL
                     TNIVMMGMGEPLYNFDHVRDALKIVMDGDGLALSKRRITLSTSGVVPMMARCGEEIGV
                     NLAVSLHAVTKEIRDEIVPINRKYGLEELLQACADYPGASNARRITFEYVMLKDKNDT
                     DAHARELVRLIRHYKLPAKVNLIPFNPWPGAIYECSAPERIRRFSDIVFEAGISAPVR
                     TPRGRDIDAACGQLKTAAQKLSRAELDRLADEKQAALG"
     gene            3993..4781
                     /locus_tag="VFF98_11280"
     CDS             3993..4781
                     /locus_tag="VFF98_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64258.1"
                     /translation="MPTRAASRPSVLITGAARRIGATLARAFGAAGWHVVLHHHDRHA
                     RAEAKALAASLPSAETVACDLLDAEAALAMVRKLAARLADWRLLVNCAAVFRFDDATA
                     LDPALFAEAMRVNAETPIRMAQAYLAPPAEGGARATGGRRVIQFTDMKVLNPNPDFFS
                     YTLAKQAMAASVKLLAMGAGGPQDRIYGLAPGAMLPSWDQAAHEHETSGRMNLLQRLT
                     LPEELAEAALFLAEGWLASGETLCIDSGQHLLAQPRDVLYLART"
     gene            4778..5374
                     /locus_tag="VFF98_11285"
     CDS             4778..5374
                     /locus_tag="VFF98_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZU64259.1"
                     /translation="MMPAFLTPLLTSTAVVTLAEIGDKTMLLAIVLAARMRRPWAIVA
                     GIFCATLVNHLISAFIGSEVAGLLSARWFQFAVAAGFIAMAGWTLVPDKLDDDEAEGA
                     VHSHGGAFLTTLVSFFLVEIGDKTQIATIALGARFHDVGAVAAGTTLGMMIADAPAVF
                     LGDRLVRIVPLKLARAVAAALFAGIGIWQLWGLLAGQA"
     gene            5474..6685
                     /locus_tag="VFF98_11290"
     CDS             5474..6685
                     /locus_tag="VFF98_11290"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZU64260.1"
                     /translation="MSDIKRVVLAYSGGLDTSVILKWLQVTYNCEVVTFTADLGQGEE
                     LEPARAKAELMGIKPEHIYIDDLREEFVRDFVFPMMRANARYEGDYLLGTSIARPLIS
                     KRLIEIARETGADAVSHGATGKGNDQVRFELSAYALEPGIKVIAPWREWDLTSRTALI
                     AWAEKHQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWSEVPDYVYSRTVNPEDAP
                     DAPEYIEIDFEKGDGVALNGVAMSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRG
                     MYETPGGEIYARAHRGIESITLDRGAAHLKDELMPKYAELIYNGFWFSPEREMLQAAI
                     DHSQAKVSGTVRLKLYKGSASVVGRKSPHSLYSERHVTFEDDAGAYDQKDAAGFIKLN
                     ALRLKLLANQK"
     gene            complement(6735..7070)
                     /locus_tag="VFF98_11295"
     CDS             complement(6735..7070)
                     /locus_tag="VFF98_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64261.1"
                     /translation="MPQGLTWRAGAMVLYVIVAQVIGASLLVKSAGFKAPGATLACVL
                     VLVSSFWMMARIIQEGLPLGLLMPMLAALVPLATIGVAVTFLGEAASWARVGVLGVAC
                     MLVGWAATL"
     gene            7182..8192
                     /locus_tag="VFF98_11300"
     CDS             7182..8192
                     /locus_tag="VFF98_11300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU64262.1"
                     /translation="MRPVCRHYGGIAAGGGAALPAMLARMPPVRPPILLFHRRYRSFQ
                     GGHLKLRDYIGHTAASGLFAPALHMAESAAGHPFADACPLWPAYRPERAAALFLAGMD
                     WDALPCGEEHRRPVINLLQGLRHADPADRRFAFLARPAIRLCVSSAVAEAVRATGQAS
                     GPVLCLPAGVDLAEIPCPASRPVAVVVAGLKRPDLARAIAARLAPALPVDCLTQPLPR
                     PAFLARLGAARIAITLPEVAEGCFLPALEAMAAGCAVICPDAGGNRDFCRDGETCLMP
                     PATPPDLADAACALLADGAWAARLAAAGRAEAARRSLAAERAAFIAVLASVASRLSPA
                     AV"
     gene            complement(8164..11064)
                     /gene="uvrA"
                     /locus_tag="VFF98_11305"
     CDS             complement(8164..11064)
                     /gene="uvrA"
                     /locus_tag="VFF98_11305"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZU64263.1"
                     /translation="MSLTHITIRGAREHNLKGVDISLPRDRLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEMMQKPDVEHIDGLSPAISIEQKTTSRNPRSTVAT
                     VTEIYDYMRLLWARIGVPYSPATGLPIAAQTVSQMVDRVMALPEGTRAYLLAPVVRGR
                     KGEYRKEIAEWQKAGYTRLRIDGELYAIEDAPALDKKYKHDIEVVVDRIAVKPGIESR
                     LADSFEQALKLADGLAFIDLADGVVPGREGEKRGKNLKGAGIPANRIVFSEKFACPVS
                     GFTLDALEPRLFSFNAPQGACPTCDGLGEKLLFDPQLVVPNEALSIKEGAVVPWAKSN
                     PPSPYYMQVLSSLAGHFGFSLTKPWADLPAEAKIVVLYGTGKQAVPLTFMDGRKSYTV
                     EKPFEGVIGNLNRRMLTTDSAWMKEELGKFQTAQPCEACEGKRLKPEALCVKVADSDI
                     SSATRLSVADAFAWFGTLDEKLGDQQRQIARAILKEINERLGFLNNVGLDYLNLDRTS
                     GTLSGGESQRIRLASQIGSGLSGVLYVLDEPSIGLHQRDNDRLLETLKRLRDLGNTVI
                     VVEHDEDAIRTADYIVDLGPGAGVHGGEVVAEGTLADILAHPDSITGQYLTGVRAIPV
                     PAERRAGNGKAITVENARANNLKGVTARFPLGTFCCVTGVSGSGKSTLTLDTLQAGAS
                     RTLNGARVIAGAHDRITGLEACDKVIEIDQSPIGRTPRSNPATYTGAFTLIRDWFAGL
                     PEAEARGYKAGRFSFNVKGGRCEACQGDGLLKIEMHFLPDVYVTCEECGGKRYNRETL
                     EVKFKGHSIADVLDMTIEDAESFFANVPPIRDRMHMLNEVGLGYVKVGQQATTLSGGE
                     AQRVKLAKELARRSTGKTLYVLDEPTTGLHFEDVRKLLEVLQRLVDQGNSVVVIEHNL
                     DVIKTADWIIDMGPEGGVRGGMVVAEGTPEDVAANPASFTGAYLKPLLEKAG"
     gene            11270..>12197
                     /locus_tag="VFF98_11310"
     CDS             11270..>12197
                     /locus_tag="VFF98_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HZU64264.1"
                     /translation="MATFALSLEDTAPLPALHMRLQRAPAVSLGRRLRVARLRQLGLR
                     MAVLLASSLSVAGLYGAQAHSEGPRPTADAAPAANTAPMVEPMPFEKAGDSFPGSAYY
                     YLSGNDSAPLLPATGDQAAHATAGPPAHPLVAALAPLDYSRALTCLTSAIYYEAATEP
                     DDGQRAVAQVVLNRLAHPAFPKTVCDVVYQGSERNTGCQFTFSCDGALARAPNRLFWE
                     RAQAVAKAALAGFVYAPVGLATHYHTFAVHPAWADSLAFIGQIGAHRFYRFAGPAGAT
                     AAFRTAYAGGEPLPGPHPRLLSAQFTAMARAGA"
BASE COUNT         1876 a         4385 c         3919 g         2017 t
ORIGIN      
        1 cttggtgtcg ttggtctcgc gcgcgccggc cgagatcgtc agcttgtcgg tgatcttgta
       61 gctggcctgg ccaaacaccg cccagctggt gttgatattg tgcagcatca cccagttatt
      121 ggggttgggc gtgccgccca gcgcgttgct ggtgaggaaa taggcgcgct gatagaattc
      181 ggtatcgtcg cggctgtcga aatagaggcc gcccagctgc cacttcagcc gcgccttgtc
      241 ggaattggcg aggcgcagct cctggctcca ctgattgagc tgggccacgc ggcccatcga
      301 ttcgctgaag ccgacattgc cgttgttgac gcccgcgccg ccatcggtgt cgccccggct
      361 atagcccgag gtgttctcgt aggcggtgat cgaggtcagg ctcaccccgc caaaatcgtg
      421 gcgcacatgc agcgactggc cccaggtggt gtaggcctgc gggttgttgt tgccctcgtc
      481 atactgcacg gcgccatagt tgaagccggc agccgggcgg ctggtgccct tctggatcga
      541 gccgcgatag aagatcgtcg ccgagccgga atagttgcga taatgggtcg acaggttgat
      601 cgaggtggcc ggctccggat cgagctgcag ctgcaggcgc acatcgcctt ccttgaagcc
      661 gcccatcaca ttgcgcccgg ccttggtgcc atcgtccgag atgccggcat aggtgttcga
      721 gatccagccc gagcgctgct gatagagcga ggacaggcgg aacttgagca catggttggc
      781 cgacagcggg ccgcccacgc cgctttccac gttcatgctg ttgtggctgc cccaggagat
      841 gctggtgtcg ccggtcatgt attcggtcgg cttggcgctt tcgaacttga tgatgccggc
      901 ggtggtgttg cggccgaaca gcgagccttg cgggcccttc agcacttcca cctggccgat
      961 atcgaaggcc ggggtggatt tgagcacgac atgctcttcc accacatcgt cctggatgat
     1021 cgagacgggc tggctggcgc cgaggtaata atcggtgttg ccaaggccgc ggatatagaa
     1081 gcgcgggaag atgcggccgg tggtcgattc cacatagagg ctgggcacct tgcccgacag
     1141 cgagagcagc gtgtcggcgc cattggcgtg atagtcttcc agcgtctcgc ccttcaccac
     1201 cgagacggcg agcggcacgt tctggaggtt ctcgctgcga tgctgggcgg tcaccaggat
     1261 cacgccattg tcatcggccg catcggtgct ggccgtatcg gtgccggcgg catgggcaag
     1321 gcccgcattg gcgatcgcga tcgcgctcac ggcagccatc gccaccgaac gaagaagtgt
     1381 catcatcaat cccttgttgc ttcaggccag ggtcacatcc cgaaggcgtg gcaaaacccc
     1441 gccgggcctc gcccatcacc tctcggacgc ggcctaggcc cgctttatga cactctgtat
     1501 aaacacgtat cccgtcgagc ccggaaacgg ccgcaatcgt ggttaaaaac gattttaccg
     1561 caggcgagtg gcttttgcgc aacagcgcca gcccgaagcg ccacacctgc cgcaccccgc
     1621 aacgcttgcc ccgcctgccc gcttctgcca agcaggccgc catgcccgcc ctgcctgccc
     1681 gccgccccct cgcagccctg gccgctgccc tttgcgccgg cgccggcctg ctggccgcgc
     1741 cccccgcgat cgcccgcgat gggctggggg tgttcggcaa ttgggcggct ttccgcgatg
     1801 gcgcggtgcc gcgctgctat gccattgccc tggccgagcc ggaacgcgcg ggcctgcgtg
     1861 gcggcaaccg gcaggccggt ctgccccgcc agttcgacgc ttatgccgat gtgggcagct
     1921 ggccgcgccg gggcgtgcgc gggcaattgc acctgcgcgc ctcgcgccgg ctgggggcag
     1981 gcgtggcaca attgtcactg ggcgggcagc gtatcgcgct gacatccggc caggcagacc
     2041 tgtgggccgc cgacccgcgc gccgatgccg ccatcatggc cgccatgcgc cagggcgggc
     2101 gcatgcaatt gctggcgcgc gatgcctcag gccacctctt caccgacagc tggcagctgg
     2161 atggcgcggc ctgcgcgatg gatgcggcca cccttgcctg cgcggccgac gcgcgccgct
     2221 agcgggcttc cagcgccgcc ccgtccagcg ccgccatatc cagcgccagc cattcggcat
     2281 gggcccgccc cggcgccacc tgcgccagca gcgcatagcg ccaccacagc cccacgcagc
     2341 gcccacccgc cagcggcgcc cgcgccagcc aggcctgcac cgccgccgct ccgctatcgc
     2401 ccgggtccgc gcccagcagg ccggccaact ggcccagatc cggcgcccca ccagcctgcg
     2461 ccgatgccgg cgccgcgcca gccagcgccc gcgcgctcac cgcctcggcc agggcgccat
     2521 cggcctggcc ggccaccgcc gcgcaagctg ccccatggcc ccgcaccagc ggcaccgcca
     2581 ccaccaccgc cagcgctgcc acaccaacgc ccgccacacc tgctttccag cccatctgtc
     2641 gcctcatggc cgcccgatag catttccagg gcaaatgcag tataggcccc ggcatgtccg
     2701 acaccacagc ctctgccccg gtggcccatc ccgccgccca catgcccatt cccggcgcca
     2761 tcgaccctgt gcccaccccg cgcgaggtga cgccccgcgc cgatggccgg gtagacctca
     2821 ccggcctgcc ctgcgccgaa atcacccgcc tgctgggcga agccgggctg gataccaagg
     2881 ccgccaaact gcgcgccaag cagctgttcc actggatcta ccaccgcggc gtcaccgact
     2941 tcgacggcat gaccgacatc gccaagacga tgcgcccctg gctggccgaa cgcttcgtca
     3001 tcggccggcc cgagatcgtc gtctcgcagc attccaccga tggcacgcgc aaatggctgc
     3061 tgcgcaccgc cgatggccac gaattcgaga tggtcttcat ccccgatgcc gaccgcggca
     3121 cgctgtgcgt ttccagccag gtgggctgca cgctcaactg ccgtttctgc cacaccggca
     3181 ccatgcgcct ggtgcgcaac ctcacccccg gcgagatcgt gggccaggtg atgctggcgc
     3241 gcgatgcgct gggcgaatgg cccaaggccg gccaggccga tttcagcgag ctgggcgatg
     3301 atctggtgga cgacaacggc ggctattcgg ccgacgggcg cctgctcacc aatatcgtga
     3361 tgatgggcat gggcgagccg ctctacaatt tcgatcatgt gcgcgatgcg ctgaagatcg
     3421 tcatggatgg cgatggcctg gccctgtcca agcggcgcat caccctgtcc accagcggcg
     3481 tggtgccgat gatggcgcgc tgcggcgagg agatcggcgt caacctcgcg gtctcgctgc
     3541 atgccgtcac gaaggagatc cgcgacgaga tcgtgccgat caaccgcaaa tacgggctgg
     3601 aggaactgct gcaggcctgc gccgattatc ccggcgccag caatgcccgg cgcatcacct
     3661 tcgaatatgt gatgctgaag gacaagaacg acaccgacgc gcatgcccgc gaactggtgc
     3721 ggctgatccg ccactacaag ctgccggcca aggtcaacct catccccttc aacccttggc
     3781 cgggcgccat ctacgaatgt tcggcgcccg aacgcatcag gcgcttttcc gacatcgtct
     3841 tcgaggcggg catttcggcg ccggtgcgca ccccgcgcgg gcgcgacatc gatgccgcct
     3901 gcggccagct gaaaaccgcc gcgcaaaagc tcagccgggc agagctggac cggctggccg
     3961 acgagaagca ggcggcgctg ggctaggcgc ccatgcccac gcgcgccgcc tcgcgcccct
     4021 ccgtgctgat caccggtgcc gcccggcgca ttggcgcaac gctcgcgcgg gcgtttggcg
     4081 cggcgggctg gcatgtggtg ctgcaccacc atgatcgcca tgcccgcgcc gaggccaagg
     4141 cgctggccgc cagcctgccc tcggccgaaa ccgtggcctg cgacctgctg gatgccgaag
     4201 cggcgctggc aatggtgcgc aagctggccg cgcgcctcgc tgattggcgc ctgctggtga
     4261 attgcgcggc ggtgttccgc ttcgatgatg ccacagcgct tgatcccgcc ctgtttgcag
     4321 aagcaatgcg cgtgaatgcg gaaaccccca tccgcatggc gcaggcctat ctcgccccgc
     4381 ccgcagaagg cggcgcgcgg gccacaggcg gccgccgggt gatccagttc accgacatga
     4441 aggtgctgaa ccccaacccc gatttcttca gctacacgct ggccaagcag gccatggccg
     4501 ccagcgtgaa gctgctggcg atgggcgcgg gcgggccgca ggatcgcatc tatggcctgg
     4561 cccccggcgc catgctgccc agctgggacc aggccgcgca tgagcatgag acctcgggcc
     4621 ggatgaacct gctgcaacgc ctgaccctgc cggaagaact ggccgaggcc gcgctgttcc
     4681 tcgccgaggg ctggctggcc agcggcgaga cgctgtgcat cgattccggc cagcacctgc
     4741 ttgcccagcc gcgcgacgtg ctttacctcg cccgcacatg atgcccgctt tcctcacgcc
     4801 gctcctcact tccaccgccg tcgtcaccct ggctgaaatc ggcgacaaga cgatgctgct
     4861 ggccatcgtg ctggccgccc gcatgcgccg cccctgggcg atcgtggcgg gcattttctg
     4921 cgccacgctc gtcaaccacc tcatctctgc cttcatcggc agcgaagtgg cagggctgct
     4981 ttccgcgcgc tggttccagt ttgccgtggc cgccggcttc atcgccatgg ccggctggac
     5041 cctggtgccc gacaagctgg acgatgacga ggccgagggc gccgtgcaca gccatggcgg
     5101 cgctttcctc accaccctcg tctccttctt cctcgtcgag atcggcgaca agacgcagat
     5161 cgccaccatc gcccttggcg cgcgcttcca cgatgtgggg gcagtggccg ccggcaccac
     5221 gctgggcatg atgatcgccg atgcgccggc ggtgttcctg ggagacaggc tggtgcggat
     5281 cgtgccgctg aagctggcac gggcggtggc agcggcgctg tttgccggca tcggcatctg
     5341 gcaattgtgg ggcctgctgg ccggccaggc ctgattgccg cgcccggccc taaggcctct
     5401 tccaccttgc gcgcccctgc cccccctgct aggcgccgcg ccaatttccc acgcaatgcg
     5461 gagcgcgcct tccatgtccg atatcaagcg ggtcgttctc gcctattccg gcggccttga
     5521 cacttcggtg atcctgaaat ggcttcaggt gacctataat tgcgaggtcg tcaccttcac
     5581 ggccgatctg ggccagggcg aggagctgga gccggcccgc gccaaggccg agctgatggg
     5641 catcaagccc gagcacatct atatcgacga cctgcgcgag gaattcgtgc gcgatttcgt
     5701 cttcccgatg atgcgcgcca atgcccgtta cgagggcgac tatctgctgg gcaccagcat
     5761 tgcccgcccg ctgatctcca agcggctgat cgagattgcg cgtgagaccg gcgccgatgc
     5821 cgtgtcgcat ggcgccaccg gcaagggcaa tgatcaggtg cgcttcgagc tgtcggccta
     5881 tgcgctggag ccgggcatca aggtgatcgc cccctggcgc gagtgggatc tcaccagccg
     5941 caccgcgctc atcgcctggg ccgaaaagca ccagatcccc gtgcccaagg acaagcgcgg
     6001 cgaaagcccc ttcagcaccg atgccaacct gctccacacc tcgtctgaag gcaaggtgct
     6061 ggaagatccg tggtcggaag tgccggatta tgtctacagc cgcacggtga accccgagga
     6121 cgcgcccgac gcgcccgaat atatcgagat cgacttcgag aagggcgatg gcgtggcgct
     6181 gaacggcgtg gcgatgagcc cggcgacgct gctggccgcg ctgaacgatc tgggccgcaa
     6241 gcatggcatc ggccggctcg atctggtcga gaaccgtttc gtcggcatga agagccgcgg
     6301 catgtatgaa acgccgggcg gcgagatcta tgcccgcgcg catcgcggca tcgaatcgat
     6361 caccctggat cgcggcgcgg cgcatctgaa ggatgagctg atgccgaaat atgccgagct
     6421 gatctacaac ggcttctggt tcagccccga gcgcgagatg ctgcaggctg ccatcgacca
     6481 ttcgcaggcc aaggtttcgg gcacggtgcg cctgaagctg tataagggca gcgcttcggt
     6541 cgtgggccgc aagtcgcccc actcgctcta ttccgaacgc catgtgacgt tcgaggacga
     6601 tgccggcgcc tatgaccaga aggacgcagc cggcttcatc aagctgaacg ccctgcgcct
     6661 gaagctgctg gccaaccaga agtaagcccc ggccgcgcgg gggaagcgtg ccttcccccg
     6721 cgcggcgcgg ccgttcacag cgtggccgcc cagcccacca gcatgcaggc cacccccagc
     6781 acgcccacgc gcgcccagct ggccgcctcg cccaggaaag tgaccgccac gccgatcgtt
     6841 gccagcggca ccagcgctgc cagcatcggc atcagcaggc ctagcggcag cccctcctgg
     6901 atgatccgcg ccatcatcca gaacgaggac accagcacca gcacgcaggc cagcgtggcc
     6961 cccggcgcct tgaagccggc gcttttcacc agcagcgatg cgccgatcac ctgcgccacg
     7021 atcacataga gcaccatcgc cccggcgcgc caagtcagcc cttgcggcat agatcccttc
     7081 ctctccttgg ggcgcggcgc tggccgccgc tgcccggtgg cggcaaaggt aatgcaagcg
     7141 cggggctgcg ggcccctcgc caaaacgcca gtgcggccag agtgcgcccc gtgtgtcgcc
     7201 attatggcgg catcgctgct ggcggcgggg cagccctccc tgccatgctg gcgcgcatgc
     7261 cgcctgttcg cccgcccatc ctgctgttcc atcgccgcta ccgcagcttt cagggcggcc
     7321 atctgaagct cagggactat atcgggcaca ccgccgcctc cggcctgttc gcccccgcgc
     7381 tgcacatggc tgaaagcgcc gccggccatc cgtttgccga tgcctgcccg ctgtggcccg
     7441 cctatcggcc agagcgggcc gccgccctct tccttgccgg gatggactgg gacgcgctgc
     7501 cctgcggcga ggaacaccgc cgcccggtga tcaacctgct gcaaggcctg cgccatgccg
     7561 acccggccga ccgccgcttt gccttcctcg cccgccccgc catccgcctg tgcgtgagct
     7621 ccgcggtggc agaggccgtg cgcgccacgg ggcaggcgtc aggcccggtg ctgtgccttc
     7681 ccgccggggt ggatctggcc gagatcccct gcccggcttc ccggcccgtg gcagtggtgg
     7741 tggcggggct gaaacggccc gatctggccc gcgcgatcgc cgcgcgcctg gcgcctgccc
     7801 tgccggtgga ttgcctgacc cagccgctgc cgcgccccgc cttcctcgcc cggctggggg
     7861 cggcgcgcat cgccatcacc ctgcccgaag tggcggaggg ctgctttctc cccgcgctgg
     7921 aagcgatggc cgccggctgc gcggtgatct gccccgatgc cggcggcaac cgcgatttct
     7981 gccgcgatgg cgagacctgc ctgatgccgc ccgccacgcc cccggatctg gccgatgccg
     8041 cctgcgccct gctagccgat ggcgcctggg ccgcgcgcct cgccgctgcc ggccgggccg
     8101 aggcggcccg ccgcagcctt gcggcagagc gggccgcctt tatcgccgtg ctggcctctg
     8161 tcgctagccg gctttctcca gcagcggttt gagataagcg ccggtgaagc tggccgggtt
     8221 ggccgccaca tcctcgggcg tgccctcggc caccaccatg ccgccacgca cgccgccttc
     8281 gggccccatg tcgatgatcc agtccgccgt cttgatgaca tcgaggttgt gctcgatcac
     8341 caccaccgaa ttgccctgat ccaccagccg ctgcagcacc tccagcagct tcctcacatc
     8401 ctcgaaatgg aggcccgtgg tcggctcgtc cagcacatag agcgtcttgc cggtggagcg
     8461 gcgggccagt tccttggcca gcttcacccg ctgcgcctcg ccgcccgaca gggtagtggc
     8521 ctgctggccc accttcacat agccaaggcc cacctcgttc agcatatgca tccggtcgcg
     8581 gatgggcggc acattggcga agaagctttc ggcatcctcg atcgtcatgt ccagcacatc
     8641 ggcgatggaa tggcccttga acttcacttc cagcgtctcg cggttgtagc gcttgccgcc
     8701 acattcctcg caggtcacat acacgtcggg caggaagtgc atctcgatct tgaggaggcc
     8761 atcgccctgg caagcctcgc agcgcccgcc cttcacgttg aagctgaagc gccccgcctt
     8821 gtagccgcgc gcctccgctt ccggcaggcc agcgaaccag tcgcggatga gggtgaaggc
     8881 gccggtgtag gtcgccgggt tcgaccgcgg cgtgcggccg atcggtgact ggtcgatctc
     8941 gatcaccttg tcgcaggcct ccagcccggt gatccggtca tgcgcgccgg cgatcacccg
     9001 ggcgccgttc agcgtgcggc tggcgccggc ctgcaaggtg tccagcgtca gcgtcgattt
     9061 gccgctgccc gaaacgcccg tcacacagca gaaggtgccc agcgggaagc gcgccgtcac
     9121 ccccttcaga ttgttggcgc gggcattttc cacggtgatc gccttgccat tgccggcgcg
     9181 cctttcggcc ggcacgggga tggcgcgcac gccggtgaga tactggccgg tgatgctgtc
     9241 gggatgggcg aggatatcgg ccagcgtgcc ctcggccacc acctcgccgc catgcacgcc
     9301 cgcccccggg cccagatcga cgatataatc ggccgtgcgg atggcatcct cgtcatgctc
     9361 caccacgatc accgtattgc cgaggtcgcg caggcgcttc agcgtttcca gcagccggtc
     9421 attgtcgcgc tggtgcaggc caatgctggg ctcatccagc acataaagca cccccgacag
     9481 gcccgagccg atctggctgg ccaggcggat gcgctgcgat tcgccgcccg acagcgtgcc
     9541 cgaggtgcga tcgaggttga gataatcgag cccgacattg ttgaggaagc ccagccgctc
     9601 gttgatctcc ttgaggatgg cgcgggcgat ctggcgctgc tggtcgccca gcttttcatc
     9661 cagcgtgccg aaccaggcaa aggcatcggc cacagacagg cgcgtggcac tggaaatgtc
     9721 gctatcggcc actttcacac acagcgcttc aggcttcagg cgcttgccct cgcaggcctc
     9781 gcagggctgg gcggtctgga acttgcccag ctcttccttc atccaggcgc tgtcggtcgt
     9841 cagcatgcgg cggttcagat tgccgatcac gccctcgaag ggcttctcca ccgtatagct
     9901 cttgcggcca tccataaagg tcagcggcac ggcctgcttg ccggtaccat acagcacgac
     9961 gatcttcgcc tcggccggca gatccgccca gggcttggtc aggctaaagc cgaaatggcc
    10021 ggcgaggctg gagagcacct gcatgtaata ggggctgggc gggttcgatt tggcccaggg
    10081 caccaccgcc ccctccttga tcgacagcgc ctcgttgggc accaccagct gcggatcgaa
    10141 caacagcttc tcgcccaggc catcgcaggt ggggcaggcc ccctgcggcg cgttgaagga
    10201 aaacaggcgc ggctccagcg catccagcgt gaagccggaa accgggcagg cgaatttctc
    10261 ggaaaagacg atgcggttgg ccgggatgcc ggcgcccttc aggttcttgc cgcgcttctc
    10321 gccctcgcgc ccgggcacca cgccatcggc cagatcgata aaggccagcc cgtctgccag
    10381 cttcagcgcc tgctcgaaac tgtcggccaa gcggctctcg atgcccggct tcacggcgat
    10441 gcggtcaacc accacctcga tgtcatgctt gtatttcttg tcgagcgcgg gcgcgtcctc
    10501 gatcgcgtaa agctcgccat cgatgcgcag gcgggtgtag ccggccttct gccactcggc
    10561 gatttccttg cgatattcgc ccttcctgcc gcgcaccacc ggcgccagca gataggcgcg
    10621 cgtgccctcg ggcagggcca tcacccggtc caccatctgg ctcaccgttt gcgccgcgat
    10681 cggcaggccg gtggccggcg aataaggcac gccgatgcgc gcccacaaaa ggcgcatata
    10741 gtcgtagatc tcggtcaccg ttgccacggt ggagcgcggg ttgcggctgg tggtcttctg
    10801 ctcgatggag atcgccggcg acaggccgtc gatatgctcg acatcgggct tctgcatcat
    10861 ttcgaggaac tggcgcgcat aggccgacag gctctcgaca tagcggcgct ggccctcggc
    10921 atagatcgtg tcgaaggcca ggctggattt gcccgagccc gaaaggccgg tgatgacgat
    10981 cagcctgtcg cgcggcaggc tgatgtccac gcccttgaga ttgtgttccc gcgcgccgcg
    11041 aatggtgatg tgggtgagac tcatggagcc ggcttgttcc agatatgttc gaccgatgca
    11101 acggggaaag cctgcccaca aaggcaggcc gggcaggcgc cggcaccggt aaaacccgcc
    11161 agcgccggtg tgccgggatg gaacaggccg aaatcggcgg atgcgcccgc gcccaagccg
    11221 ggttagcggc aaggctcgcc gccggatctg ccggcgcagg gattgcatca tggccacctt
    11281 cgccctctcg cttgaagaca ctgcccccct gcccgcgctc catatgcggc tgcagcgggc
    11341 gccggctgtg tcgctggggc ggcggctgcg ggtggcgcgg ctgcgccagc tggggctgcg
    11401 catggcggtg ctgctggcca gcagcctgtc ggtggccggg ctctatggcg cgcaggcgca
    11461 tagcgagggc ccccgcccca ccgccgatgc cgcccccgcc gccaataccg cgccgatggt
    11521 ggagccgatg cccttcgaaa aggccggcga cagcttcccc ggctctgcct attactacct
    11581 ttccggcaat gacagcgcgc cgctgctgcc cgccaccggc gatcaggccg cccatgccac
    11641 cgccggcccg ccggcgcatc cgctggtggc agcgctggcg ccgctcgatt acagccgcgc
    11701 gctcacctgc ctcaccagcg ccatctatta cgaagccgcc accgagcccg acgatggcca
    11761 gcgcgccgtg gcgcaggtgg tgctgaaccg cctggcccac cccgccttcc ccaagacggt
    11821 gtgcgatgtc gtctaccagg gctcggagcg caacaccggc tgccagttca ccttcagctg
    11881 cgatggcgcg ctggcccgcg cgcccaaccg cctgttctgg gaacgcgcgc aggcggtggc
    11941 aaaggcggcg ctggccggct ttgtctatgc gccggtgggg ctggccaccc attaccacac
    12001 cttcgccgtg caccccgcct gggccgacag cctggccttc atcggccaga tcggcgcgca
    12061 tcgcttctat cgctttgccg gccctgcggg cgccaccgcc gccttccgca ctgcctatgc
    12121 cggcggcgag ccgctgcccg gcccccaccc gcgcctgttg agcgcgcagt tcaccgccat
    12181 ggcccgcgcg ggcgccg
//
LOCUS       DASRYE010000222         6941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_257501,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000222 DASRYE010000000
VERSION     DASRYE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257501"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFF98_11315"
     CDS             complement(<1..941)
                     /locus_tag="VFF98_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU64265.1"
                     /translation="MRAPLAKQRPKLLVVEDDAGLQAQLKWAYEDYTVITAADRPSAI
                     AALRAEMPAVVTLDLGLPPDPDGTSEGFALLDEIMALKPDTKVIVASGHGARESALQA
                     IARGAYDFYQKPVNIDDLGLIVARAFRLHGLEAENRQLAQRGGEGHQVLGEMITAAPE
                     MLKVARTIERVAPTNVSVMLLGASGTGKELLARGLHEASDRREGAFIAINCAAIPESL
                     LESELFGHEKGSFTGAVKTTEGKIEMADGGTLFLDEVGDIPLALQVKLLRFLQERVIE
                     RIGGRRAIAVDTRIVCATHQNLEAMIAEGRFREDLWYR"
     gene            complement(941..3019)
                     /gene="prsK"
                     /locus_tag="VFF98_11320"
     CDS             complement(941..3019)
                     /gene="prsK"
                     /locus_tag="VFF98_11320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HZU64266.1"
                     /translation="MMGGHAVWAGVGDATHALGAFACVIALGWLWQRRGPLPAVQRAS
                     ALSLAATALWCLAVAAEGSQSLAAGLVQALRNLAYLGTVYALFAGDGRHTSVAQVRPV
                     MAALAMVAVMQPLVLLAQYRLAGELGPAAYQANTLMALLQVVGSLVLLHNLYAGGSNA
                     ARQLLRWPVLGLAAVWVFELNLYTAAYLGDGWPEALAALHGLADTALAAGLALGTARG
                     HAGLRLRPSRAVAFRSVSLLLIGVYLAGMVAVARGLAVIGGGHARWLQFGFLILASAL
                     VVLALPSRRLRGWLRITLTKHLFEHRYDYRAEWLRFSATIGGRGADAPPLYQRAIQAL
                     ADITDSPGGLLLLPDEEGEMVLADRWRWPEMVVPAPAFPRIGAHFAQRMDFIVDFDAW
                     RGAGAGQAAVPAWLMAEPAAWAMVPLLHAEQLVGAVILARPPHSRRIDWEDFDLLRIV
                     GRHLGTYLAEHHSQQALAEAARFDEFNRRMAFVMHDIKNLASQFALLARNAERHAENP
                     AFRADMLITLRASADKLNTLVSRLSRYGGPAPDRVAPVSLGAVARGVAARFGGAATVS
                     VVEREEGLVLAQAEALEQALMHLVQNAVDASPAGRPVYLFVCREGAEGRIDVQDNGCG
                     MSADFLRTRLFRPFVSTKPGGFGIGTFEAREMVRAMHGRLDAESREGLGSCFTIRLPL
                     AAAGDVAQVA"
     gene            complement(3021..4406)
                     /locus_tag="VFF98_11325"
     CDS             complement(3021..4406)
                     /locus_tag="VFF98_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HZU64267.1"
                     /translation="MLRLFKHYIPHAVLLLGLVDLVLLVLAGEMAWRLRSDQLGVYAG
                     TIDSRLRSLAVFAAMILTAMIAVGVYGAQALRSLRFSSARVMVAVSLGIIALSALCQI
                     LGGHDFWRSTLLYAMGLAIGFLVLNRMLLGEVIGAHAFRRRVLVLGAGERAQRLRQLG
                     EAPEAGFAIVGYVGMSDKPPVVEEAIARAAIHNLKRHVENLGVSEVVLALEERRGALP
                     LKDLLRIKTAGVHVNDFASFIERETGRVDLDSVNPSWLIFSDGFSAGRAFSSTTKRLF
                     DVLVSLTLLGLTAPVIALFAVIVKLDSRGPAFFRQTRVGLYGQPFDVIKLRSMRLDAE
                     AGGAQWASQNDPRVTRVGRFIRKVRIDELPQAWSVLKGDMSFVGPRPERPEFVADLEE
                     QLRYYAERHMVKPGITGWAQVNYPYGASIEDSHNKLEYDLYYVKNYTPFLDVLIILQT
                     LRVILWPEGAR"
     gene            complement(4587..5990)
                     /locus_tag="VFF98_11330"
     CDS             complement(4587..5990)
                     /locus_tag="VFF98_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HZU64268.1"
                     /translation="MSISSSTVGPAIRRLRRQQGLTQTAMAGRLQISPSYLNLIERGQ
                     RPITPRLVEQMVELFDFDPRALRGEDVIGGMAGLERRLADDRFADLAIAPQDIAEWLA
                     AAPQTAVAFARLYDASASGVEQAAALDPLVLARREIERWRNHFPALEAAAEALAGELD
                     LPAADTTAALAGHLAGRHGFAVEVVPVAAMPDALRRVDMAARVLQLSEMLDLPARHFR
                     MARQIALLEQREAIAAIANGAHFADRAAWRLFRRHLAAYFAAALLMPYGRFLAACEET
                     GYDLAVLQRRFSVSFEQLAHRLTTLQRPGARGLPFFMARLDRAGQYSKRYAAGSGALL
                     LEGEASCPKWAVHTAFATGSPQAQLVRARDGAGRLGQWLTLARPVAKPDLPAEPGAAR
                     QAAHFVVVLGVEARLAGRLAAGGALLSAGQGAIVVGPGCIHCAEAGCAQRAQPPRGAS
                     LTFDERVEGPMPFALRP"
     gene            complement(6118..>6941)
                     /locus_tag="VFF98_11335"
     CDS             complement(6118..>6941)
                     /locus_tag="VFF98_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU64269.1"
                     /translation="VNCAAIPESLLEAELFGVEKGAFTGATAPRAGRFERAEGGTLFL
                     DEVGTLSLAAQGKLLRAIQEGEYERVGGERVHRADVRLVAATNVDLRTAVREGHFRAD
                     LFYRLNVFPIRIPPLRERRADLPLLMEHFLDTYAARYRKQITGFEEEAVAAILGYDWP
                     GNIRELENVIERGVIMARDGDPLRLHHLFGGGEVLGPPAFRVGPEGRVADRAEAPGPD
                     EALAALMAAGMDLAGLERRMIDQALARAGGNKSRAAAALGITRAQLYYRLKEQGQ"
BASE COUNT         1217 a         2549 c         2234 g          941 t
ORIGIN      
        1 cgataccaca gatcctcgcg gaagcgcccc tcggcaatca tcgcctccag gttctggtga
       61 gtggcgcaca cgatccgcgt gtccaccgca atcgcccgcc gcccgccgat ccgctcgatc
      121 acccgctcct gcaggaagcg cagcagcttc acttgcagcg ccagcggaat atcgcccacc
      181 tcgtcgagga acagcgtgcc gccatcggcc atctcgatct tgccctcggt ggttttcacc
      241 gcccccgtga acgagccctt ttcatggccg aacagttccg attccagcag gctttcggga
      301 atggccgcgc aattgatggc gatgaacgcc ccctcgcgcc ggtcggaagc ctcgtgcagg
      361 ccgcgcgcca gcagttcctt gccggtgccg ctggccccca gcagcatcac cgagacattg
      421 gtgggcgcca cccgctcgat ggtgcgggcc actttcagca tttcgggcgc ggcggtgatc
      481 atctcgccca gcacctggtg cccctcgccg ccgcgctggg ccagctggcg gttttccgcc
      541 tccagcccgt gcaggcggaa ggcgcgcgcc acgatcagcc ccagatcatc gatgttcacc
      601 ggcttctggt agaaatcata ggcaccgcgg gcgatcgcct gcagggcgct ttcgcgcgcg
      661 ccatggccag aggcgacgat gaccttggta tcgggcttca gcgccatgat ctcgtcgagc
      721 agggcaaagc cctcgctggt gccatcagga tcgggcggca ggcccagatc gagcgtgacc
      781 accgccggca tttccgcccg cagcgccgcg atcgccgagg ggcgatcagc agcggtgatc
      841 accgtgtaat cctcataggc ccatttgagc tgcgcctgga ggccggcatc atcctccacc
      901 accagcagct tggggcgctg ttttgccagg ggcgcgcgca tcaggccacc tgtgccacat
      961 cgccggctgc tgccagcggc aggcggatgg tgaagcagct gcccagcccc tcgcgcgatt
     1021 cggcatccag ccggccgtgc atggcgcgca ccatctcgcg cgcctcgaaa gtgccgatgc
     1081 caaagccgcc gggcttggtg gaaacgaacg ggcggaacag gcgcgtgcgc aggaaatcgg
     1141 cactcatgcc gcagccattg tcctgcacat cgatgcggcc ctcagccccc tcgcggcaga
     1201 cgaacagata gaccgggcgg ccggcggggc tggcatccac ggcgttttgc accagatgca
     1261 tcagtgcctg ctccagcgct tcggcctggg ccagcaccag cccctcctcg cgctccacca
     1321 ccgagaccgt ggccgccccg ccaaagcgcg ccgccacccc ccgcgccacc gcccccagcg
     1381 acaccggcgc cacccgatcg ggcgccggcc cgccatagcg cgacaggcgg ctgaccagcg
     1441 tgttcagctt gtcggccgag gcgcgcaggg tgatcagcat gtcggcgcgg aaggccgggt
     1501 tttcggcatg gcgctcggca ttgcgggcga gcagcgcaaa ctggctcgcc aaattcttga
     1561 tatcatgcat gacaaaggcc atccgccggt tgaactcgtc gaagcgggcg gcttcagcca
     1621 gggcctgctg gctgtggtgt tcggccagat aggtgcccag atggcggccg acgatacgca
     1681 ggagatcaaa atcctcccag tcgatccggc ggctgtgcgg cgggcgggcc aggatcaccg
     1741 cgcccaccag ctgctcggca tgcagcagcg gcaccatcgc ccaggcggcc ggctctgcca
     1801 tcagccaggc gggcaccgcc gcctgcccgg ccccggcgcc ccgccaggca tcgaaatcga
     1861 cgatgaaatc catccgctgc gcaaaatgcg cgccaatgcg cgggaaggca ggcgccggca
     1921 ccaccatctc gggccagcgc cagcgatcgg ccagcaccat ctcgccctcc tcgtcgggca
     1981 gcagcagcag gccgccgggg ctgtcggtga tatcggccag ggcctggatc gcgcgctggt
     2041 agagcggcgg cgcatcggcg ccccgcccgc caatcgtggc ggaaaaccgc agccattcgg
     2101 cgcgatagtc gtaacggtgc tcgaacaggt gtttggtgag cgtgatccgc agccagccgc
     2161 gcagccgccg cgagggcagc gccagcacca ccagcgccga ggcgaggatg agaaagccga
     2221 actgcagcca gcgcgcatgc ccgccgccga tcaccgccag cccgcgcgcc accgccacca
     2281 tgccggccag atacacgccg atcagcagca gcgacaccga gcggaacgcc accgcccgcg
     2341 atggccgcag ccgcagcccc gcatggccgc gcgccgtgcc cagcgcgagg cctgccgcca
     2401 gcgccgtatc ggccagccca tgcagcgcgg ccagcgcctc gggccagcca tcgcccagat
     2461 aggcggcggt gtagaggttc agctcgaaca cccagaccgc cgccagcccc agcaccggcc
     2521 agcgcagcaa ttgccgcgcc gcattggaac cgccggcata aagattgtgc agcagcacca
     2581 gcgagccgac cacctgcagc agcgccatca gcgtgttggc ctgataggcc gccgggccca
     2641 gctcgcccgc cagccggtat tgcgccagca gcaccagcgg ctgcatcacc gccaccattg
     2701 ccagcgcggc catcaccggg cgcacctggg ccacgctggt gtggcgccca tcgccagcga
     2761 acagggcata gaccgtgccc agataggcca ggttgcgcag cgcctgcacc agcccggccg
     2821 ccaggctctg gctgccctcg gcggccacgg ccaggcacca cagcgccgtg gcggcaaggc
     2881 tgagcgcgct ggcccgctgc accgccggca agggcccccg ccgctgccac agccagccca
     2941 gcgcgatcac gcaggcaaaa gcgcccagcg catgggtggc atcgcccacc cccgcccaga
     3001 ccgcatgccc gcccatcatg ctagcgcgcc ccttccggcc acaggatcac ccgcagcgtt
     3061 tgcaggatga tcagcacgtc gagaaagggt gtgtaatttt ttacataata gagatcatac
     3121 tcaagcttgt tgtgcgaatc ctcgatcgag gcgccatagg gataattcac ttgcgcccag
     3181 ccggtgatgc ccggcttcac catgtgccgc tcggcgtaat agcgcagctg ctcttccaga
     3241 tcggccacga attccgggcg ttccggccgg gggccaacga agctcatatc gcccttcagc
     3301 acgctccagg cctggggcag ttcatcgatg cgcaccttgc ggatgaaccg gccaacgcgg
     3361 gtgacgcgcg gatcgttctg gctggcccat tgggcgccgc ccgcttctgc atccagccgc
     3421 atggagcgca gcttgatgac atcgaaaggc tggccataaa ggcccacacg cgtctgccgg
     3481 aagaaggccg ggccgcggct gtccagcttc acgatcacgg cgaacaaggc gatcaccggc
     3541 gccgtcaacc ccagcagggt gaggctgacg agaacatcga acaggcgctt ggtcgtgctg
     3601 gaaaaggcgc gcccggccga aaaaccgtcg gagaagatca gccagctggg gttcacgctg
     3661 tcgagatcga cgcggcccgt ctcgcgctcg atgaagctgg caaaatcatt gacatgcacg
     3721 cccgccgtct tgatccgcag caggtctttc aggggcagcg cgccgcgccg ttcctccagc
     3781 gccagcacca cttcggaaac gcccagattt tccacatggc gcttcagatt gtggatcgcc
     3841 gcacgggcaa tggcctcttc caccaccggc ggcttgtcgc tcatgcccac atagcccacg
     3901 atggcaaagc cggcttcggg cgcctcgccc agctggcgca ggcgctgggc ccgctcgccc
     3961 gcgcccagca ccagcacccg ccggcggaag gcatgggcgc cgatcacctc gcccagcagc
     4021 atgcggttga gcaccagaaa gccgatcgcc agccccatcg catagagcag ggtggagcgc
     4081 cagaaatcat gcccgcccaa gatctggcac agggccgaaa gggcgatgat gcccagcgac
     4141 accgccacca tcacccgcgc cgaggaaaac cgcagcgagc gcagcgcctg cgcgccataa
     4201 acgcccaccg cgatcatcgc tgtgaggatc atcgcggcaa acaccgccag cgagcgcagg
     4261 cggctgtcga tcgtgccggc ataaacgccc agctgatcgg agcgcagccg ccaggccatc
     4321 tcgccggcca gcaccagcag caccagatcg acaaggccca gcagcagcac ggcatggggg
     4381 atataatgtt tgaagaggcg cagcattggt ggggcaaacc tgttccagcc ggggccatgc
     4441 cccacccctg cttagggcaa agccgtaaac tgtcggtaaa actgacaggc gacaatcggc
     4501 ggcccgcata aaaatcgtgg cttgccagcc agatgggccg cagccctgtt aaccagggcg
     4561 acagaaggcc ggttcagggg gcaggttcag gggcgcaggg caaacggcat cggtccttcc
     4621 acccgctcgt cgaaagtgag gctggcgccg cggggcggct gggcgcgctg ggcgcagccg
     4681 gcctccgcgc aatggatgca gcccgggccc accacgatcg ccccctgccc cgccgacagc
     4741 agggcgccgc ccgccgccag ccgcccggcc agccgcgcct ccacccccag caccacgacg
     4801 aaatgcgccg cctgccgcgc cgcgcccggt tctgccggca gatcgggctt ggccaccggc
     4861 cgcgccagcg tcaaccactg gcccagccgg ccggcaccat cacgcgcgcg caccagctgg
     4921 gcctgggggc tgccggtggc aaaagcggtg tgcaccgccc atttggggca gctggcctca
     4981 ccctccagca gcagcgcgcc gctgccagcg gcatagcgct tggaatattg ccccgcccta
     5041 tccagccgcg ccatgaagaa gggcaggccg cgcgcacccg gccgctgcag ggtggtcaac
     5101 cggtgggcca gctgctcgaa gctgaccgag aagcgccgct gcagcaccgc cagatcatag
     5161 cccgtctcct cgcaggcggc gaggaaccgg ccatagggca tcagcagggc agcggcgaaa
     5221 taggccgcga gatggcggcg gaacaggcgc caggccgccc gatcggcaaa atgggcgcca
     5281 ttggcgatgg ccgcgatcgc ctcgcgctgc tccagcagag caatctggcg cgccatgcgg
     5341 aaatggcggg ccggcagatc cagcatttcc gacagttgca gtacgcgcgc cgccatatcc
     5401 acccggcgca gcgcatcggg catggccgcc accggcacca cttccaccgc aaagccatgc
     5461 cgcccggcca gatggccggc cagcgcggcg gtagtgtcgg ccgcgggcag gtccagctcg
     5521 cccgccagcg cctcggcggc cgcttccagc gcggggaaat gattgcgcca gcgctcgatc
     5581 tcgcggcgcg ccagcaccag cggatcaagc gcggcggcct gctccacccc gctggccgag
     5641 gcatcgtaga ggcgggcgaa ggccaccgcc gtctggggcg cggcggccag ccattcggca
     5701 atgtcttgcg gcgcgatggc aaggtcggcg aagcggtcgt cggccagccg ccgttccagc
     5761 ccggccatgc cgccaatcac atcctcgccg cgcagcgcgc gcgggtcgaa atcgaacagt
     5821 tccaccatct gttcaacgag gcgcggcgtg atcggccgct gcccccgctc gatcaggttg
     5881 aggtagctgg gcgagatctg cagccgcccg gccatcgctg tctgggtgag gccctgctgg
     5941 cggcgcagcc ggcggatggc ggggccgaca gtggaggaag agatgctcat gggcagaagc
     6001 gtgtcatgct ttttacaagc caacaagcac ctgaaaaaga ataataatct tcacatattc
     6061 aaggctttga acaggccctt tcgggccccc taggggggaa tccggggcgc tgtggtgcta
     6121 ttgcccctgc tccttcagcc gatagtaaag ctgcgcccgg gtgatgccca gcgcggcggc
     6181 ggcgcggctc ttgttgcccc cggcgcgggc cagcgcctgg tcgatcatcc gccgctccag
     6241 cccggccaga tccatccctg cggccatcag cgcggccagc gcctcgtcgg gcccgggtgc
     6301 ttccgcccgg tcggccacgc gcccttccgg ccccacgcgg aaggccggcg gccccaacac
     6361 ctcgcccccg ccaaacaggt ggtgcagccg caagggatcg ccatcgcgcg ccatgatcac
     6421 cccgcgctcg atgacatttt ccagctcgcg gatattgccc ggccagtcgt aacccaggat
     6481 cgccgccacc gcctcttcct cgaagccggt gatctgcttg cgatagcgcg cggcataggt
     6541 gtcgaggaaa tgctccatca gcagcggcag atcggcccgc cgctcccgca agggcgggat
     6601 gcggatggga aagacgttga gccggtagaa caggtcggcg cggaaatggc cctcgcgcac
     6661 cgccgtgcgc agatcgacat tggtggccgc caccagccgc acatcggcgc ggtgcacccg
     6721 ctcgccgccc acgcgctcat attcgccctc ctggatggcg cgcagcagct tgccctgcgc
     6781 cgccagcgac agcgtgccca cctcgtcgag gaacagcgtg cccccctcgg cccgttcgaa
     6841 gcggccggcg cgcggggcgg tggcgccggt gaaggcgccc ttctccacgc cgaacagttc
     6901 cgcttccagc aggctttccg ggatggccgc gcaattgacc g
//
LOCUS       DASRYE010000223        12893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_13334,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000223 DASRYE010000000
VERSION     DASRYE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13334"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1000
                     /gene="rsmI"
                     /locus_tag="VFF98_11340"
     CDS             173..1000
                     /gene="rsmI"
                     /locus_tag="VFF98_11340"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZU64270.1"
                     /translation="MDQPLLPGLYIVATPIGNLGDITLRAIEVLRGVDAVACEDTRVT
                     GRLMQHLGLKKRLIRYDDHAEETAREGLLALMESQAVALVSDAGTPLVSDPGYRLVKE
                     ARARGLAITSLPGPCAAIMGITLAGLPSDRFLFAGFLPPREKARAEALAELAAVPASL
                     VFYETAPRLLDTLAAIGAGLPGREMAVARELTKLFEECRTGTPDALIAHYTAHPPKGE
                     IVLVVGPPVARAASMADAEALLRAELAHAKPSQAAAAVARATGLDRKALYQLALDLK"
     gene            997..1368
                     /locus_tag="VFF98_11345"
     CDS             997..1368
                     /locus_tag="VFF98_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZU64271.1"
                     /translation="MNWRRAADRIAADAHGRRGEEEAARWLAAQGFALLARRVKTPRG
                     EVDLIARGEGLVVFVEVKWRARAGDLAQAIDQRRLARVAAAAEVLAPHYVKPGEDMRI
                     DVLLLAPGHDPHHIANAWMPL"
     gene            1381..2385
                     /gene="gshB"
                     /locus_tag="VFF98_11350"
     CDS             1381..2385
                     /gene="gshB"
                     /locus_tag="VFF98_11350"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZU64272.1"
                     /translation="MSLRVAVQMDPLHAINVAGDSTFHMMLAAQARGHQLWYYDVATL
                     AWQSQGAGAGRITAWAAPVSVRRPEAAGESHYTLGAFADIDLGADIDVVLMRQDPPFD
                     LGYITGAHLLEKLKGTTLVVNDPAEVRNAPEKVFVLDFARFMPPTLVTRRIEDVRAFQ
                     ARMLAEGLGGDLVIKPLHGNGGKAVFRVPASGDNLGALVEVFGQMWKEPFMVQPFLPD
                     VAKGDKRIVLVDGEVAGAINRRPGEGEFRSNLAVGGSAEASELTEREAEICAALGPEL
                     RARGLVFVGIDVIGGQWLTEINVTSPTGIVAIDRFNGSDVGGLIWQAIERRHKELAGA
                     "
     gene            2382..3482
                     /locus_tag="VFF98_11355"
     CDS             2382..3482
                     /locus_tag="VFF98_11355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZU64273.1"
                     /translation="MNPPAPARLDQLTSLRFFAALAVLASHLWPLAEEPNLLQPVART
                     LFFEGFAGVSFFFMLSGFILAHTYQAKLATGAISKRKYLALRIARIAPLHWLVAAPFA
                     VAALVAGGLAEAPKVGLNLALLQSWVPDADWYFTLNEPSWSLSDEAFFYVCFAFLAFW
                     PVRRLGLLAAGLLLADGAVAAWRVAGGHGAILVDGRNTFTHWLTYIDPAARLLDFVWG
                     MLLYRLPRKGGSTWLEALALALLFDAMASAQALGLAEAWRMQLAYLPYMALLIWTFAQ
                     GGGWFSRVLARTRWLVLLGDASFALYLVHLPVVHAGLWLQDRLDEAALPWLPWAAGVS
                     MIAIALSVAVYRWVEVPLLRASRRWIDAVFAR"
     gene            complement(3479..4384)
                     /locus_tag="VFF98_11360"
     CDS             complement(3479..4384)
                     /locus_tag="VFF98_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZU64274.1"
                     /translation="MNDLAARAALVARWQAHLAAERRRSPHTVRAYVATARRLLEASG
                     AESWPALAALKAPELRDFLARRRAAGLANVSAAREMAAMAAFIAFARREAGLPEEPMP
                     LRRPRVKRGLPRPVTPDEAVALAEGVAEEASAPWIGLRDRAVLLLLYGAGLRIAEALA
                     LSAGALPPGETLRITGKGGKQRLVPILPQVAAALAEYAAACPFPLPPAEPLFRGARGG
                     PLQAALVRRAVARLRGALGLPDTATPHALRHSFASHLLGAGADLRSLQELLGHASLSS
                     TQIYTKVDAARLLDTYRAAHPRAKD"
     gene            complement(4381..5529)
                     /gene="hemW"
                     /locus_tag="VFF98_11365"
     CDS             complement(4381..5529)
                     /gene="hemW"
                     /locus_tag="VFF98_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZU64275.1"
                     /translation="MARALYIHWPFCLKKCPYCDFNSHVRAGVDIALWQRALLADMAH
                     EAEHAGGEPLESIFFGGGTPSLMPPALVATLLAEAEKRWGFAPNIEITLEANPSSVEA
                     AHFAALASAGVNRVSLGVQALDDAALRFLGRLHGVDEALAALAVAQAHFARVSCDLIY
                     ARPGQSAAAWEAELTRALGFGTSHLSLYQLTIEPGTRFATDVRAGAFTPLAEDPAADL
                     FTLTRQITAACGLPAYEVSNHARPGAESRHNLAYWRYQDYAGIGPGAHGRRGGKATLR
                     HKKPENWLAGITETGHGLAEERPLPPREQAAEALLMGLRLAEGIAPAALAARFGLAEA
                     QLIDPAKAALYAAQGLVWRRADRLGVTEAGMLLLDALIAELAAEGLAA"
     gene            complement(5538..6164)
                     /gene="rdgB"
                     /locus_tag="VFF98_11370"
     CDS             complement(5538..6164)
                     /gene="rdgB"
                     /locus_tag="VFF98_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZU64276.1"
                     /translation="MARLSGRLVIATHNAGKLREIAALLAPYGLDCVSAGDLGLPEPA
                     ETGTTFAENAALKARAAASASGLPALADDSGLCVAALGGRPGVYTADWAERQWFEGEQ
                     GRDWYMAMGKVEGLLAEQGPEVDRSGYFACTLALAWPDGALAVYEGRVHGQLTWPPRG
                     KLGFGYDPVFVPQGHAQTFAEIAPEAKHAISHRADAFARLVADQFGPD"
     gene            complement(6168..6884)
                     /gene="rph"
                     /locus_tag="VFF98_11375"
     CDS             complement(6168..6884)
                     /gene="rph"
                     /locus_tag="VFF98_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZU64277.1"
                     /translation="MRPSGRAPDEMRPIEIVTHFTKHAEGSVLISFGDTRVLVTASVE
                     ERVPPFLRGKGEGWVTAEYSMLPRATHTRGQREAAKGKQSGRTQEIQRLIGRSLRAVT
                     DLKKLGERQITLDCDVLQADGGTRTAAISGAWVALRIAVDKLMASGHITEDPLTRQVA
                     AVSCGICNGTPVLDLDYVEDSAADADANFVLISGGHIAEAQATAEGATYDEESLLRLL
                     RLARIGCEGIFAEQAKAVGR"
     gene            6980..8038
                     /gene="hrcA"
                     /locus_tag="VFF98_11380"
     CDS             6980..8038
                     /gene="hrcA"
                     /locus_tag="VFF98_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZU64278.1"
                     /translation="MTSLPITELNARAREVFRLVVEGYLESGQPVGSKRLAGLGSGLG
                     VSSATIRGVLAELEGMGLLSHPHTSAGRMPTEVGLRLFVDGIMQVAEPTLEEREAIAR
                     SVAEPGPIEAALAATSAALSGLSACAGVVMVPAREPHLAQVSLVPLGPGRALAVLVDE
                     EGGIENRLLNLPLSLPPGALEQASNYITARLAGRTLADAAGAIQAEITSGESALDAAA
                     ADLVRRGLAVWSEDGLAEPGRRPVLIVRGAANLLDEAAITDLERVRSLLDDLETKQSV
                     ASLLDVAREAQSTRIFIGSENRLFALSGSSVIASPYRDRDGRVVGVVGVIGPTRLNYA
                     RIVPMVDFTAQSLGKLIG"
     gene            8054..8596
                     /gene="grpE"
                     /locus_tag="VFF98_11385"
     CDS             8054..8596
                     /gene="grpE"
                     /locus_tag="VFF98_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZU64279.1"
                     /translation="MTTDTPQPQDEVAVDGQNTGETDTATRLAALEAELEAARNDVLY
                     ARADTQNVRRRLEKDVADARAYAATGFARDILPVADNLARALEAIPADLREDEKVKAV
                     VAGIEATVRELEKVFGVHGIAKVAAHGLPLDPNQHQAMMEVPHGEAEPGTVLQVLQHG
                     YTIKERLLRPAMVVVAKKPD"
     gene            complement(8651..9157)
                     /locus_tag="VFF98_11390"
     CDS             complement(8651..9157)
                     /locus_tag="VFF98_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZU64280.1"
                     /translation="MKYFPLATAALLLSACHSQKAEQAPAAPASANLVITGGRLVLPA
                     VPGHPAAAYFSVLNLSHAPVTITGVQLADAASAEMHQTSGDTMAALPRLEIAGDGSAI
                     FSPGGRHVMIFGLSPKVKAGQNEELTLTIANAKPVTLPLHVESAGGTDMSGMASSASS
                     DAMASMKM"
     gene            9413..11317
                     /gene="dnaK"
                     /locus_tag="VFF98_11395"
     CDS             9413..11317
                     /gene="dnaK"
                     /locus_tag="VFF98_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZU64281.1"
                     /translation="MGKVIGIDLGTTNSCVAVMDGGKPKVIENSEGARTTPSIVAFTK
                     DGERLIGQPAKRQAVTNPDNTIFAVKRLIGRRYDDPMTQKDMALVPYKIAKGKNGDAW
                     VEAGGSDYSPSQVSAFTLQKMKETAESYLGESVTQAVITVPAYFNDAQRQATKDAGQI
                     AGLEVLRIINEPTAAALAYGLDKNDGKTIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDTKLVEYLADKFKAKEGLDLKTDRLALQRLKEAAEKAKIELSSAQTTEI
                     NLPFITARMEGGMTTPLHLVETVSRADLERLVADLIERTMEPCRKALADAGIKASDID
                     DVVLVGGMTRMPKVRDAVKAFFGKEPHTGVNPDEVVAMGAAIQAGVLQGDVKDVLLLD
                     VTPLSLGIETLGGVFTRMIDRNTTIPTKKSQVFSTADDNQQAVTIRVFQGEREMAADN
                     KILGQFDLIGIPAARRGVPQIEVTFDIDANGLVNVSAKDKGTGKEQQIRIQASGGLSD
                     SDIDQMVRDAEKFAEEDKKRRASAEARNNADSLAHATEQQLAEHGDKIDAGLKAEIEA
                     AIAEVKAASEAGNVEDITAKTQALTEKAMKLGQAIYEKEQAAAAAPGAQPGGEDVVDA
                     EFSEVDEKKG"
     gene            11395..12537
                     /gene="dnaJ"
                     /locus_tag="VFF98_11400"
     CDS             11395..12537
                     /gene="dnaJ"
                     /locus_tag="VFF98_11400"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZU64282.1"
                     /translation="MSIEIDYYELLEVNREADDATIKSSFRKLAMKCHPDRNPGCKDS
                     EAKFKQINEAYECLKDPQKRAAYDRFGHAAFQQGGPGGPGGGFGGGQEFGDIGDIFET
                     IFGSAFGGGGGGRQQARRGADLRYDLEIGLEEAFHGKSLEIEVDVAAPCEPCKGSGAE
                     PGTGARRCNMCGGHGKVRAQQGFFMVERTCPTCHGRGEVIEKPCRACQGEGRVDKRQK
                     LEVNIPRGVDSGTRIRLAGKGEAGPFGAPPGDLYIFLHLRRHAMFDRDGTTLIAHVPV
                     SFTTAALGGEIEIPGLDGQRHAIPIPAGTQSGKQLRKRGAGMPVLNGRGVGDMVVEVV
                     VRTPEKLTARQKEILREFQSTETGEECPEVKGFFERLKDAWNDLTE"
     gene            complement(12581..>12893)
                     /locus_tag="VFF98_11405"
     CDS             complement(12581..>12893)
                     /locus_tag="VFF98_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64283.1"
                     /translation="VGLVIVALAHRWQAGPRLQKIAATLGLMTYPFYLFHQDVGAILL
                     GLLRRAGLAHAPALMAQLALLLAMTLAITLWAEPLLRRALAALLAWRPGGRTGQPAIR
                     T"
BASE COUNT         2023 a         4162 c         4683 g         2025 t
ORIGIN      
        1 agcgccatcg ccggcccccg ggccttgcgc cacgccgctg cgcgcgaaag ggcgggcagg
       61 cgggcaggct tgcggttctt ggcagtcatg gcgtcactct tccctgatgg catcgcggcg
      121 cggcggcctt gatgcccttg cgttgggcgg gggggctggt ccatgtgggg ctatggatca
      181 acccttgctt cccggtctct atattgtcgc cacgccgatt ggcaatctgg gcgatatcac
      241 cttgcgcgcg atcgaggtgc tgcgtggcgt cgatgcggtg gcctgtgagg acacgcgggt
      301 gacaggccgg ctgatgcagc atctgggcct gaagaagcgc ctgatccgct acgatgatca
      361 tgccgaggaa accgcgcgcg aagggctgct ggcgctgatg gaaagccagg cggtggcgct
      421 ggtgtcggat gccggcacgc cgctggtgtc ggatccgggc taccggctgg tgaaggaggc
      481 gcgtgcgcgc ggccttgcca tcaccagcct gccggggcct tgcgcggcga tcatgggcat
      541 cacgctggcg ggcctgccgt cggatcggtt tctgttcgcc ggcttcctgc cgcccaggga
      601 aaaggcgcgg gccgaggcgc tggccgaact ggccgccgtg cccgccagcc tggtgttcta
      661 cgaaaccgcg ccgcgcctgc tcgacacgct ggcggcgatc ggcgccgggc tgccgggccg
      721 cgagatggcc gtggcgcgcg agctgaccaa gctgttcgag gaatgccgca cgggcacgcc
      781 cgacgcgctg atcgcccatt acaccgccca tccgcccaag ggcgagatcg tgctggtggt
      841 gggcccgccg gtggcgcgcg cggccagcat ggccgatgcc gaggcgctgc tgcgcgccga
      901 actggcccat gccaagccca gccaggccgc cgctgcggtg gcccgcgcca ccgggctgga
      961 caggaaggcg ctctatcagc tggcgcttga tctcaaatga actggcgccg cgcggccgac
     1021 cggatcgccg ccgatgccca tggccggcgc ggcgaggaag aagctgcccg ctggctggcc
     1081 gcgcaggggt ttgccctgct ggcccggcgg gtgaaaaccc cgcgcggcga ggtggatctg
     1141 atcgcccggg gggaagggct ggtggtcttc gtcgaggtga aatggcgggc ccgcgccggg
     1201 gatctggccc aggcgataga ccagcggcgc ctcgcccgcg tggccgccgc cgccgaggtg
     1261 ctcgcgcccc attatgtaaa accgggcgaa gacatgcgga tagatgttct tctgcttgcg
     1321 cccggccacg atccgcacca catcgccaat gcgtggatgc cgctttagga gaatgattgc
     1381 atgtcgctgc gtgtcgccgt ccagatggac cctttgcatg cgatcaatgt cgccggcgat
     1441 tccacctttc acatgatgct ggccgcccag gcccgcggcc accagctgtg gtattacgat
     1501 gtggcaacgc tggcctggca gtcgcagggc gcgggggcag gccgcatcac cgcctgggcc
     1561 gcgccggttt cggtgcgccg gcccgaagct gcgggcgaga gccactacac gctgggcgcc
     1621 tttgccgata tcgaccttgg cgccgatatc gatgtggtgc tgatgcggca ggatccgccg
     1681 tttgatctgg gctatatcac cggcgcccat ctgctggaaa agctgaaggg caccacgctg
     1741 gtggtgaacg acccggccga ggtgcgcaat gcgcccgaga aggttttcgt gctcgatttc
     1801 gcgcgcttca tgccgcccac gctggtcacc cggcggatcg aggatgtgcg cgctttccag
     1861 gcccgcatgc tggccgaagg gctgggcggc gatctggtca tcaagccgct gcatggcaat
     1921 ggcggcaagg cggtgttccg cgtgccggcc agcggcgaca atctgggcgc gctggtggaa
     1981 gtgttcggcc agatgtggaa ggagcccttc atggtgcagc ctttcctgcc ggatgtggcc
     2041 aagggcgaca agcgcatcgt gctggtggat ggcgaggtgg ccggcgccat caaccgccgc
     2101 ccgggcgagg gggagttccg ctcgaacctc gccgtgggcg gcagcgccga ggccagcgag
     2161 ctgaccgagc gcgaggccga gatctgcgcc gcgctggggc cggaactgcg cgcgcgcggg
     2221 ctggtgttcg tgggcatcga tgtgatcggc ggccagtggc tgaccgagat caacgtgacc
     2281 agccccaccg gcatcgttgc catcgaccgg ttcaatggca gcgatgtggg cggtctgatc
     2341 tggcaggcga tcgagcggcg ccacaaggag ctggccgggg cgtgaaccca cccgcacctg
     2401 cccgcctcga ccagctgaca tcgctgcgtt tcttcgcggc gctggcggtg ctggcctcgc
     2461 atctgtggcc gctggccgaa gagcccaacc tgctgcagcc ggtggcccgc acgctgttct
     2521 tcgaggggtt tgccggggtc agcttcttct tcatgctgtc gggctttatc ctggcgcata
     2581 cctatcaggc aaagctggcc acaggggcga tctcgaagcg caaatatctg gccttgcgca
     2641 ttgcccgcat cgccccgctg cattggctgg tggccgcgcc ctttgcggtg gcggcgctgg
     2701 tggcgggcgg gctggcagag gcgcccaagg tgggcctgaa cctggccctg ctgcaaagct
     2761 gggtgcccga tgccgactgg tatttcacgc tgaacgagcc gagctggagc ctgtcggacg
     2821 aggcgttctt ctatgtctgc tttgcctttc tggccttctg gccggtgcgg cggctggggt
     2881 tgctggcggc gggcctgctg ctggccgatg gcgcggtggc ggcatggcgg gtggctggcg
     2941 ggcacggcgc cattctggtg gatgggcgca acaccttcac ccattggctg acctatatcg
     3001 acccggctgc gcggctgctc gatttcgtct ggggcatgct gctttaccgg ttgccgcgca
     3061 agggcggcag cacctggctg gaagcgctgg cgctggcgct gctgttcgat gcgatggcca
     3121 gcgcgcaggc gctgggcctg gccgaggcgt ggcgcatgca gctggcctat ctgccctata
     3181 tggccctgct gatctggact ttcgcgcaag gcggcgggtg gttctcgcgg gtgctggcgc
     3241 gcacgcgctg gctggtgctg ctgggcgatg cgtctttcgc gctttatctc gtgcatctgc
     3301 cggtggtgca tgcgggcctg tggctgcagg accggctgga tgaggcggcg ctgccctggt
     3361 tgccctgggc ggcgggggtg agcatgatcg ccatcgcctt gtcggtggcg gtgtatcgct
     3421 gggtggaagt gccgctgctg cgtgcctcgc gccggtggat cgacgcggtg ttcgcgcgct
     3481 agtcctttgc ccgcggatgg gcggcgcgat aggtgtcgag caggcgggcg gcatccacct
     3541 tggtgtagat ctgggtggac gacaggctgg cgtggcccag cagttcctgc aggcttctga
     3601 ggtctgcccc ggcgcccagc aggtggctgg caaagctgtg gcgcagggca tggggggtgg
     3661 ccgtatcagg caggcccagc gcgccgcgca gccgcgccac cgcccggcgc accagcgctg
     3721 cctgcaaggg gccgccgcgc gcgccgcgaa acagcggttc ggccggcggc agcgggaagg
     3781 ggcaggcagc ggcatattcc gccagcgctg cggccacttg cggcaatatc ggcaccagcc
     3841 gctgcttgcc gcccttgccg gtgatgcgca gcgtttcgcc cgggggcagg gcgccggcgc
     3901 tcagcgccag tgcttcggca atgcgcaggc cggcgccata gagcagcagc agcaccgccc
     3961 tgtcgcgcag gccaatccag ggcgcgctgg cttcctccgc cacgccttcg gccagcgcca
     4021 ccgcctcgtc gggcgtcacc gggcggggca ggccgcgctt cacccgcggg cggcgcagag
     4081 gcatgggctc ttccggcagg cccgcctccc gtcgggcaaa ggcgatgaac gcggccatcg
     4141 ccgccatttc ccgcgcggcg gaaacattgg ccaggcccgc cgcccgccgc cgggcgagaa
     4201 aatcgcgcag ctctggcgcc ttcagcgcgg ccagcgccgg ccagctttcg gccccgctcg
     4261 cttccagcag gcggcgggcg gtggcgacat aggcgcgcac cgtgtggggc gagcgccgcc
     4321 gctctgccgc cagatgggcc tgccagcgcg cgacaagggc ggcgcgggcg gccagatcat
     4381 tcatgccgca aggccctcgg cggcaagctc cgcgatcagc gcgtcgagca gcagcatgcc
     4441 cgcttctgtc acccccagcc gatcggccct gcgccacacc agcccttgcg ctgcataaag
     4501 cgcggccttg gcgggatcga tcagctgcgc ctcggccagg ccaaagcggg cggcaagggc
     4561 ggcaggggca attccctcgg ccaggcgcag gcccatcagc agggcttctg cggcctgttc
     4621 gcgcgggggg agcgggcgtt cctcggccag gccatggccg gtttcggtaa tgccggccag
     4681 ccagttttcc ggcttcttgt ggcgcaaggt ggccttgccg ccgcgccggc catgggcgcc
     4741 cgggccaatc ccggcataat cctgatagcg ccaataggcg aggttatgcc ggctttcggc
     4801 gcccgggcgg gcatggttgc tcacctcata ggcgggcagg ccacaggcgg cggtgatctg
     4861 gcgggtgagg gtgaagagat cggccgccgg gtcttctgcc agcggggtga aggcgccagc
     4921 gcgcacatcg gtggcaaagc gcgtgcccgg ctcgatcgtc agctggtaga gcgagaggtg
     4981 gctggtgcca aagcccagcg ctcgggtcag ctcggcttcc catgcggcgg cgctctggcc
     5041 ggggcgggca tagatgagat cgcagctgac gcgggcgaaa tgggcctgcg ccacggcaag
     5101 ggcggccagc gcctcgtcca ccccgtgcag ccggccgagg aagcgcagcg cggcatcatc
     5161 gagcgcctgc acccccagcg acacgcgatt gaccccggcg ctggcaaggg cggcgaaatg
     5221 ggccgcttcc acgctggagg ggttggcttc cagcgtgatc tcgatgttgg gcgcaaagcc
     5281 ccagcgtttc tccgcctcgg ccagcagggt ggcgaccagc gccggcggca tcaggctggg
     5341 cgtgccgccg ccaaagaaga tgctctccag cggctcgccg ccggcgtgct cggcctcatg
     5401 ggccatgtcg gccagcaggg cgcgctgcca cagggcgata tccacgccgg cgcgcacatg
     5461 gctgttgaaa tcgcaatagg ggcatttttt caggcagaac ggccagtgga tatagagcgc
     5521 gcgggccatg ggcgcggtca atccggcccg aactgatcgg ccaccagccg ggcgaaggca
     5581 tcggcgcggt ggctgatggc gtgcttggcc tcgggggcga tttcggcaaa ggtttgcgca
     5641 tggccctgcg gcacgaagac aggatcgtag ccaaagccca gcttgccgcg cggcggccag
     5701 gtgagctggc catgcacccg cccctcatag accgccagcg ccccatccgg ccaggccagc
     5761 gccagcgtgc aggcgaaata gccgctgcgg tccacctcgg ggccctgttc ggccaggagg
     5821 ccctccacct tgcccatcgc catgtaccag tcgcggccct gctcgccctc gaaccactgg
     5881 cgctcggccc agtcggccgt gtagacgccg gggcggccgc ccagcgctgc cacgcacagg
     5941 ccgctgtcgt cggccagcgc cggcaggccg ctggcactgg ccgccgcgcg cgccttgagg
     6001 gcggcgtttt cggcaaaggt ggtgccggtt tcggccggct cgggcaggcc cagatcgccg
     6061 gcggaaacgc aatccagccc ataaggcgcc agcaaggcgg cgatttcgcg cagcttgccg
     6121 gcgttatggg tggcgatcac cagcctgccc gaaagcctgg ccatggctta gcggcccacg
     6181 gccttggcct gctcggcaaa gatgccctcg cagccgatgc gggccaggcg cagcaggcgc
     6241 agcaggcttt cctcgtcata ggtggcgccc tcggcggtgg cctgggcctc ggcgatgtgg
     6301 ccgcccgaga tcagcacgaa attggcatcg gcatcggcgg cggaatcctc cacatagtcg
     6361 aggtcgagca ccggcgtgcc gttgcagatg ccgcaggaga ccgccgccac ctggcgggtc
     6421 agcggatcct cggtgatgtg gccagaggcc atcagcttgt ccaccgcgat gcgcagcgcc
     6481 acccaggcgc cggaaatcgc cgcggtgcgg gtgccgccat cggcctgcag cacgtcgcag
     6541 tcgagggtga tctgccgctc gcccagcttc ttgaggtcgg tcacggcgcg cagcgagcgg
     6601 ccgatcagcc gctggatttc ctgcgtgcgg ccgctttgct tgcccttggc cgcttcacgc
     6661 tggccgcggg tgtgggtggc gcgcggcagc atggaatatt ccgccgttac ccagccctcg
     6721 cccttgccgc gcaggaacgg gggcacgcgc tcttccacgc tggcggtgac cagcaccctg
     6781 gtgtcgccaa agctgatcag caccgagcct tcggcgtgct tggtgaagtg cgtgacgatt
     6841 tcgatggggc gcatttcgtc gggggcgcgg ccggatgggc gcatgggtgt ctccttcttc
     6901 tcgcgcttcc cttggcgcct gcccgcctgc cgcgcaagag cgttggcgat ttattcgccg
     6961 ctggttatag caggcagcca tgaccagcct gccgatcacc gaactgaacg cgcgtgcgcg
     7021 cgaggttttc cgcctggtgg tggaaggcta tctggaaagc gggcagccgg tggggtccaa
     7081 gcgcctggcg gggctgggca gcgggctggg cgtgtcctcg gccaccattc gcggcgtgct
     7141 ggccgagctg gagggcatgg gcctgctcag ccacccccac accagcgccg ggcggatgcc
     7201 caccgaagtg gggctcaggc tgtttgtcga tggcatcatg caggtggcag aacccaccct
     7261 tgaggagcgc gaggcgatcg cccgctcggt ggcggaaccg gggccgatcg aggcggcgct
     7321 ggcggccacc agcgcggcgc tctcgggcct gtcggcctgc gcgggcgtgg tgatggtgcc
     7381 cgcgcgtgag ccgcatctgg cgcaggtgag cctggtgccg ctggggccgg gccgggcgct
     7441 ggcggtgctg gtcgacgagg agggcgggat cgagaaccgc ctgctgaact tgccgctgtc
     7501 gctgccgccc ggcgcgctgg agcaggcttc gaactacatc accgcgcggc tggccgggcg
     7561 cacgctggcc gatgcggccg gcgccatcca ggccgagatc acctcgggcg agagcgcgct
     7621 ggatgccgcc gctgccgatc tggtgcgccg cggccttgcc gtgtggagcg aggacgggct
     7681 ggccgagccc ggccgccgcc cggtgctgat cgtgcggggg gcggccaacc tgctggacga
     7741 ggcggcgatc accgatctgg aacgcgtgcg ctcgctgctc gacgatctgg agaccaagca
     7801 atcggtggcc agccttctgg atgtggcgcg cgaggcgcag tcgacgcgga ttttcatcgg
     7861 cagcgagaac cggctgttcg cgctgtcggg ctcttcggtc atcgcctcgc cctatcgcga
     7921 tcgggatggg cgggtggttg gcgttgtggg ggtgatcggc cccacgaggt tgaattatgc
     7981 gcgcatcgtc cccatggtgg atttcaccgc ccagagtttg ggcaagctca tcggataacc
     8041 ggaaacccat attatgacga cagacacccc gcagccccag gacgaggtgg ccgtggacgg
     8101 ccagaacact ggcgaaaccg ataccgccac gcggcttgcc gcgctggaag ccgaactgga
     8161 ggcggcacgc aacgacgtgc tgtatgcccg cgccgacacg cagaatgtgc gccgccggct
     8221 ggaaaaggac gtggccgatg cgcgcgccta tgccgccacc ggctttgccc gcgatatcct
     8281 gccggtggcc gataatctcg cccgcgcgct ggaggcgatc cctgcggatc tgcgcgagga
     8341 cgagaaggtg aaggcggtgg tggccggcat cgaggccacc gtgcgcgagc tggagaaggt
     8401 gtttggcgtg catggcattg ccaaggtggc cgcccatggc ctgccgctgg accccaacca
     8461 gcaccaggcg atgatggaag tgccccatgg cgaggcagag cccggcaccg tgctgcaggt
     8521 gctgcagcat ggctatacca tcaaggagcg gctgctgcgc ccggcgatgg tggtggtggc
     8581 caagaagccc gactgagcct ggccccatgg cggcgcgcgc gggcccctgg caagcgcgcg
     8641 ccggccggga tcacattttc atgctggcca ttgcatcgct gctggccgaa ctggccatgc
     8701 cggacatgtc ggtgccgccg gcgctttcca catgcagggg caaggttact ggcttggcat
     8761 tggcgatggt cagcgtaagc tcctcgttct gcccggcttt caccttgggc gagaggccga
     8821 agatcatcac atgcctgccg cccggcgaga agattgccga gccatcgccg gcgatttcca
     8881 gccggggcag ggcggccatg gtgtcgccgc tggtctggtg catttcggcg ctggcggcat
     8941 cggccagttg cacgccggtg atggtgacag gggcatgcga gaggttgagc acggagaaat
     9001 aggcagccgc cggatggccg ggcacggccg gcagcaccag ccgcccgccg gtgatcacca
     9061 gattggccga ggcaggggcc gcaggggcct gctctgcctt ctgggaatgg caggcgctga
     9121 gcagcagggc agctgtggca agggggaaat atttcacgtg acgctcccga aaccggtgct
     9181 tgcaaggcac cgcttgaacc cgattctatg gcagaggggg gcagatttgt gcaccccttt
     9241 cctcgatctt aaccacgaaa agcaaaatgg cgggtgaacc gcaggcgggg gccttgtgcc
     9301 ggcgcaaagc gctcctatat cggcccgcat atcagagccg ccatggcagt ttgccgcagt
     9361 ttcgcggggg ctgcgcgggt ggtgtcacga ataggcaagg actggaaccg ctatgggtaa
     9421 agtaatcggg atcgatctgg gcaccaccaa cagctgcgtc gccgtgatgg acggcggcaa
     9481 gccgaaggtg atcgagaatt cggaaggcgc gcgcaccacg ccctccatcg tggccttcac
     9541 caaggatggt gagcggctga tcggccagcc ggccaagcgc caggcggtga ccaaccccga
     9601 caacaccatc ttcgcggtga agcgcctgat cggccgccgc tatgacgacc ccatgaccca
     9661 gaaggacatg gcgctggtgc cctacaagat cgccaagggc aagaatggcg atgcctgggt
     9721 ggaagctggc ggcagcgatt attcgccttc gcaggtttcc gccttcaccc tgcagaagat
     9781 gaaggaaacc gccgagagct atctgggcga gagcgtgacg caggcggtga tcaccgtgcc
     9841 ggcctacttc aacgacgccc agcgccaggc caccaaggat gccggccaga ttgcgggcct
     9901 tgaagtgctg cgcatcatca acgagccgac cgcggcggcg cttgcctatg gcctcgacaa
     9961 gaacgatggc aagaccatcg ctgtctacga ccttggcggc ggcacgttcg acgtgtcgat
    10021 cctggagatc ggcgatggcg tcttcgaggt gaagagcacc aacggcgaca ccttcctggg
    10081 cggcgaggat ttcgacacca agctggttga atatctggcc gacaagttca aggccaagga
    10141 agggcttgac ctcaagaccg accgcctggc cctgcagcgc ctgaaggaag cggctgaaaa
    10201 ggccaagatc gagctgtcga gcgcgcagac caccgagatc aacctgccct tcatcaccgc
    10261 ccgcatggaa ggcggcatga cgaccccgct gcatctggtg gaaacggtca gccgcgccga
    10321 tctggagcgg ctggtggccg atctcatcga gcgcacgatg gagccttgcc gcaaggcgct
    10381 ggccgatgcc ggcatcaagg cgagcgacat cgacgatgtg gtgctggtgg gcggcatgac
    10441 ccgcatgccc aaggtgcgtg atgcggtgaa ggccttcttc ggcaaggaac cgcacaccgg
    10501 cgtcaacccg gacgaagtgg tggcgatggg cgctgccatc caggccggcg tgctgcaggg
    10561 cgatgtgaag gatgtgctgc tgctcgacgt gaccccgctg tcgctgggca tcgagacgct
    10621 gggcggcgtg ttcacccgca tgatcgaccg caacacgacg atccccacca agaagagcca
    10681 ggtgttctcg actgctgacg acaaccagca ggcggtgacg atccgcgtct tccagggcga
    10741 gcgcgagatg gccgcggaca acaagatcct cggccagttc gatctcatcg gcattccggc
    10801 ggcgcggcgc ggcgtgccgc agatcgaggt gaccttcgat atcgacgcga acggcctcgt
    10861 caacgtgtcg gccaaggaca agggcaccgg caaggagcag cagatccgca tccaggcctc
    10921 gggcggcctg tcggacagcg acatcgacca gatggtgcgc gatgccgaga agtttgccga
    10981 ggaagacaag aagcgccgcg cttcggccga ggccaggaac aacgccgaca gcctggccca
    11041 tgccaccgag cagcagctgg ccgagcatgg cgacaagatc gatgcgggcc tgaaggccga
    11101 gatcgaggcc gccatcgccg aggtgaaggc cgcgagcgag gccggcaatg tggaggacat
    11161 caccgccaag acccaggccc tgaccgaaaa ggccatgaag ctgggccagg cgatctatga
    11221 gaaggagcag gccgctgctg ctgcccccgg cgcccagccg ggcggtgagg atgtggtcga
    11281 cgccgaattc tcggaagtgg acgaaaagaa gggctgatcg ccccgttgac acaagcttcc
    11341 gccgtctctg ccccggccgg gtaggggcgg cggttgcctt tcagggatcg ggccatgtcc
    11401 atagagatcg actattacga attgctggaa gtgaaccgcg aggcggacga tgcgacgatc
    11461 aagtcctcgt tccgcaagct ggcgatgaaa tgccacccgg atcgcaaccc cggctgcaag
    11521 gattccgagg cgaagttcaa gcagatcaac gaggcctatg agtgcctgaa ggatccgcaa
    11581 aagcgcgccg cctatgatcg tttcggccat gccgccttcc agcagggcgg gcctggcggg
    11641 cccggcgggg gctttggcgg cggccaggaa tttggcgata tcggcgatat tttcgagacc
    11701 atctttggca gcgcctttgg cggcggcggg ggtgggcgcc agcaggcgcg gcgcggggcg
    11761 gacctgcgct atgaccttga gatcgggctg gaagaggcct tccacggcaa atcgctggag
    11821 atcgaggtgg atgtggccgc cccttgcgag ccgtgcaagg gatcgggcgc ggaacccggc
    11881 accggcgccc ggcgctgcaa catgtgcggc ggccatggca aggtgcgcgc gcagcagggc
    11941 ttcttcatgg tggagcgcac ctgccccacc tgccacggcc gcggcgaggt gatcgaaaag
    12001 ccctgccgcg cctgccaggg cgagggccgg gtggacaagc gccagaagct ggaagtgaac
    12061 attccgcgcg gggtggatag cggcacgcgc atccgcctgg ccggcaaggg cgaggcgggg
    12121 ccgtttggcg cgccgccggg cgatctgtat atctttctcc acctgcgccg ccacgccatg
    12181 ttcgaccgcg atggcaccac gctgatcgcc catgtgccgg tgagctttac caccgcggcg
    12241 ctgggcggcg agatcgagat ccccggcctt gatggccagc gccatgccat ccccattccc
    12301 gccggcacgc aaagcggcaa gcagctgcgc aagcgcggcg ccggcatgcc ggtgctgaac
    12361 gggcgcggcg tgggcgatat ggtggtggaa gtggtggtgc gcacgcccga gaagctgacg
    12421 gcccggcaga aggagattct gcgcgagttc cagtctaccg agaccggcga ggaatgcccc
    12481 gaggtgaagg gctttttcga acggttgaag gatgcctgga acgatctgac cgaataggcg
    12541 ggcacaaagg ggcgggggcg atgttccccg cccctttttg tcaggtgcgg atggcgggct
    12601 ggccggtgcg cccgcccggc cgccaggcca gcagggccgc cagggcacgg cgcagcagtg
    12661 gttcggccca cagggtgatc gccagcgtca tcgccagcag cagcgccagc tgggccatca
    12721 gcgccggcgc atgggccagc ccggcccggc gcagcagccc cagcagaatg gcaccgacat
    12781 cctgatggaa gaggtagaag gggtaggtca tcagccccag cgtggcggcg atcttctgca
    12841 ggcgggggcc tgcctgccag cgatgagcca gcgccacgat caccagacca acc
//
LOCUS       DASRYE010000224         6088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_154567,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000224 DASRYE010000000
VERSION     DASRYE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154567"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /gene="sppA"
                     /locus_tag="VFF98_11410"
     CDS             <1..1768
                     /gene="sppA"
                     /locus_tag="VFF98_11410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZU64284.1"
                     /translation="VRLNGTLVEEPQAQDPASVLLGNGEAPAQTRARDVERALALAAG
                     DSQVKAVVVDLAGFSGSSGLVTLEEVGAAMDRVRAAGKPVLVYGTMLEDAGLLLAAHG
                     SEVWLDPLGAAWAPGPGGYHLYYGKLLEKLKITAHIFRVGTYKDFVEPYFRNDQSEPS
                     RAAREALYQAIWAEWRANVGHARPKARIDLASADPVGLMKAAGGDAAKAAQSAGLIDR
                     IGTRADFGARVAAIAGADGASKRPGAYAHTGLAAYLARHPESRAGKAIGVVTVAGDIV
                     DGNEGPGTAGGARIARLIDDSAAKNLAALVVRVDSPGGSVTGAEAIRAAILRAKARGL
                     PVVVSMGDMAASGGYWVATPGSRIFARPGTITGSIGIFAVIPSFEKALAAWGVSGDGV
                     RTTPLSGQPDVFTGLTPQMEQLVQAEIEGGYARFVGLVAASRHKSPEAVDAIAQGRVW
                     DGASARGNGLIDAYGGLDEALADAASQAHLGAGAWHADYLGSEKSAFAQWLAQIAPDD
                     GTSDSGDGDAPQDLAGLVADRQQRELAGMLGDVAMLAGSKGAKAYCLECSAALLAPRP
                     LPAGGWPGWLGLAGQVLAAFRR"
     gene            1871..1955
                     /locus_tag="VFF98_11415"
     tRNA            1871..1955
                     /locus_tag="VFF98_11415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1905..1907,aa:Leu,seq:tag)
     gene            2013..3545
                     /gene="tig"
                     /locus_tag="VFF98_11420"
     CDS             2013..3545
                     /gene="tig"
                     /locus_tag="VFF98_11420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZU64285.1"
                     /translation="MQIVETSSEGLKRAYTVTIPAGVIAEKVESEVVRLAPQVRLPGF
                     RPGKVPVNLLKKMHGAQLHQEALSGSIREALDKVVAENALRPATQPDVQLAEGYEEGK
                     DAELTVALEVLPTIATPDLSGLKLEKLVVPVTDEQVSEAVERIASSAKRFVDAPEGKE
                     AETGDQLTIDFLGKLDGVPFDGGKAEDAALEIGAGRFIPGFEEQLAGAKAGDERVITV
                     TFPEDYQAAHLAGKEATFDITVKAVKVAGETVIDDEFAKSLGLTDLAQLQGLLRGQLE
                     QETAGLTRTQMKRQLLDILAEGHDFDVPPSMVEAEFGQIWQQLTHEASHEENPEEALA
                     EIEAEKDDYRAIAVRRVRLGLLLSEIGQSNGVEVTAQEMQMLVSQAAQQYRPEDRQRF
                     AEYIQNEPLAAAQLRAPLYEEKVVDFLFDQAEVAEREVTKDELQAAIEADENGPAEHV
                     HGPDCNHDHDHDHEAAKAEDAAPKKKAAPKKKAEAAEGEDAAEPKKKAAKKKAAEEAG
                     EA"
     gene            3835..4521
                     /gene="clpP"
                     /locus_tag="VFF98_11425"
     CDS             3835..4521
                     /gene="clpP"
                     /locus_tag="VFF98_11425"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZU64286.1"
                     /translation="MIDLFGHAGSNAQGKFHVDPVTGSLIPVVVEQSSRGERSFDIYS
                     RLLRERIIFVTGQIEDHMASVIVAQLLFLESENPSKDISMYINSPGGVVTAGLAIYDT
                     MKYIRPKVSTVCIGQACSMGSFLLAAGEPGMRIALPSARIMVHQPSGGAQGMASDIEI
                     QAREILRIRKRMNDLYVKFTGRSLEEIENAMDRDTFLEADEALKFGLIDKVFETRPNA
                     DEISGEAGKE"
     gene            4631..5887
                     /gene="clpX"
                     /locus_tag="VFF98_11430"
     CDS             4631..5887
                     /gene="clpX"
                     /locus_tag="VFF98_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZU64287.1"
                     /translation="MTKLSGSDTKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     NDIIREESKSGIAGKKDGGVPTPRDIFETLNDYVIGQDRAKRVLSVAVHNHYKRLKHS
                     GKGGEVELSKSNILLVGPTGSGKTLLAQTLAKTFDVPFTMADATTLTEAGYVGEDVEN
                     IILKLLQASDYNVEKAQHGIVYIDEIDKISRKAENPSITRDVSGEGVQQALLKLMEGT
                     TASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIIADRLQKRSIGFGAHVADP
                     DKRKVGELLQKAEPEDLLKFGLIPEFVGRLPVIATLEDLDIPALVRILREPKNALVKQ
                     YAKLFELEDVALTFTEEALEAIAKKAIERKTGARGLRSIVEGLLLDTMFDVPTETGIA
                     EVVVDKDVVEGRKEPVRVLKAKEEAA"
BASE COUNT         1086 a         1895 c         2190 g          917 t
ORIGIN      
        1 ggtgcggctg aacggcacgc tggtggaaga gccgcaggcg caggatccgg ccagcgtgct
       61 gctgggcaat ggcgaggcgc cggcccaaac ccgcgcccgc gatgtggagc gggcgctggc
      121 gctggcggcc ggtgattcgc aagtgaaggc ggtggtcgtc gatctggccg gcttttcggg
      181 cagttcgggc ctcgtcacgc tggaggaagt gggcgcggcg atggaccgcg tgcgcgccgc
      241 cggcaagccg gtgctggttt atggcaccat gctggaggat gcagggctgc tgctggccgc
      301 ccatggcagc gaggtgtggc tcgatccgct gggggcggcc tgggcgccgg ggccgggggg
      361 ctatcacctc tattacggca agctgctgga aaagctgaag atcaccgcgc atatcttccg
      421 cgtgggcacc tacaaggatt tcgtcgaacc ctatttccgc aacgaccagt cggaaccctc
      481 gcgcgctgcg cgtgaggcgc tttaccaggc gatctgggcg gagtggcggg ccaatgtcgg
      541 ccatgcccgg cccaaggcgc gcatcgatct ggccagcgcc gatccggtgg gcctgatgaa
      601 ggcggccggc ggcgatgcgg ccaaggcggc gcaatcggcc gggctaatcg accggattgg
      661 cacccgggcc gattttggcg cccgcgtggc cgccatcgcc ggggcagacg gggccagcaa
      721 gcggcccggc gcctatgccc acaccggcct tgccgcctat cttgcccgcc atccggaaag
      781 ccgggcgggc aaggcgattg gcgttgtcac cgtggcgggc gatatcgtgg atggcaatga
      841 agggccgggc acggcgggcg gcgcgcgcat cgcccggctg atcgacgatt cggcggccaa
      901 gaatctggcg gcgctggtgg tgcgggttga ttcgccgggc ggctctgtca ccggggcgga
      961 ggcgattcgc gcggccatcc tccgggccaa ggcgcgcggc ctgccggtag tggtgtcgat
     1021 gggcgatatg gcggccagcg gcggctattg ggtggcaacg ccgggcagcc gcatctttgc
     1081 ccgcccgggc acgatcaccg gcagcatcgg catcttcgcg gtgatcccca gctttgagaa
     1141 ggcgctggcg gcctggggcg tttccggcga cggggtgcgc accacgccgc tttccggcca
     1201 gcccgatgtg ttcaccggcc tcacgccgca gatggagcag ctggtgcagg ccgagatcga
     1261 ggggggctat gcccgctttg tggggctggt ggccgcctcg cgccacaaga gccccgaagc
     1321 ggtggatgcc atcgcccagg ggcgcgtgtg ggatggcgcc agcgcgcggg gcaacgggct
     1381 gatcgacgcc tatggcgggc tggacgaggc gctggccgat gccgccagcc aggcccatct
     1441 gggcgcgggc gcctggcatg ccgattatct gggcagcgaa aaatccgcgt ttgcccaatg
     1501 gttggcgcag atcgccccgg atgatggcac cagcgattcc ggcgatggcg atgccccgca
     1561 ggatctggca gggctggtgg cagaccggca gcagcgggaa ttggccggca tgctgggcga
     1621 tgtggcgatg ctggcgggca gcaagggggc caaggcctat tgcctggaat gttcggccgc
     1681 gctgctggcg cccaggccgc tgccggcggg cggctggccg ggctggctgg ggctggcggg
     1741 gcaggtgctg gcggcgttcc ggcgctgacg gcggctctgc tgaatcgccc cgcggcgcaa
     1801 tgcccgcttg ccctttgccc tgcgcccgac taagggcctc ggcttgattt ttcgtggctt
     1861 cgcatctgac gcgggcgtgg cggaactggt agacgcgctg gatttaggtt ccagtatcgc
     1921 aagatgtggg ggttcaagtc ccttcgcccg caccagtttt ccgcccggtg tcaggggttt
     1981 ggccgccggc gaatggaagg cgtttgagga tcatgcagat tgtcgagacc agcagcgaag
     2041 ggctgaagcg cgcctatacg gtgaccattc ccgcaggggt gatcgccgag aaggtggaaa
     2101 gcgaagtggt gcgcctggcg ccgcaggtgc gcctgcccgg cttccgcccc ggcaaggtgc
     2161 cggtcaacct gctcaagaag atgcacggcg cccagctgca ccaggaagcg ctttcgggct
     2221 cgatccgcga ggcgctggac aaggtggtgg ccgagaacgc cctgcgcccg gccacccagc
     2281 ccgacgtgca gctggccgag ggctatgagg aaggcaagga cgccgagctg accgtggcgc
     2341 tggaagtgct gccgacgatc gccacgccgg atctttcggg cctgaagctg gaaaagctgg
     2401 tggtgccggt gaccgacgag caggtgagcg aggcggtgga gcgcatcgct tcctcggcca
     2461 agcgctttgt cgacgcgccc gagggcaagg aagccgaaac cggcgaccag ctgacgatcg
     2521 acttcctggg caagctcgat ggcgtgccgt ttgacggtgg caaggccgag gacgccgcgc
     2581 tggaaatcgg cgccggccgc ttcatccccg gcttcgagga gcagctggca ggggccaagg
     2641 cgggcgacga gcgcgtgatc accgtgacct tccccgagga ttaccaggcc gcccatctgg
     2701 ccggcaagga agccactttc gacatcaccg tgaaggcggt gaaggtggcc ggcgagacgg
     2761 tgatcgacga cgagttcgcc aagagcctgg gcctgaccga tctggcgcag ctgcagggcc
     2821 tgctgcgcgg ccagctggag caggaaaccg ccggcctcac ccgcacccag atgaagcgcc
     2881 agctgctcga catcctggcc gagggccatg atttcgacgt gccgccgagc atggtggaag
     2941 ccgagttcgg ccagatctgg cagcagctga cccacgaagc cagccacgag gaaaaccccg
     3001 aggaagcgct ggccgagatc gaggccgaga aggacgacta tcgcgccatc gccgtgcgcc
     3061 gcgtgcgcct gggcctgctg ctgagcgaga tcggccagag caatggcgtg gaagtgaccg
     3121 cccaggaaat gcagatgctg gtgagccagg ccgcccagca gtatcgcccg gaagaccgcc
     3181 agcgctttgc cgaatacatc cagaacgagc cgctggctgc tgcccagctg cgcgcgccgc
     3241 tgtatgagga aaaggtcgtc gacttcctgt tcgatcaggc cgaagtggcc gagcgcgaag
     3301 tgactaagga cgagctgcag gctgccatcg aagccgatga gaacggcccg gccgaacacg
     3361 tccacggccc cgactgcaac cacgaccacg accacgacca tgaggccgcc aaggccgagg
     3421 acgctgcccc caagaagaag gccgcgccca agaagaaggc cgaggccgcc gagggcgagg
     3481 acgctgccga gcccaagaag aaggccgcca agaagaaggc tgccgaggaa gccggcgagg
     3541 cctgatcgcc tgccggcagg ccctgggccg tgccgctggc tgcaatgcac gaaggctccg
     3601 cccccgaaaa gggggtgggg cctttgtcct gagcgggggc tgccggcggc cgcggcctgc
     3661 ggaaaacccc gatgcgccgg gcaaggggca ggggcgcaag ggcgcggccg ggcgtttggc
     3721 caatccgttt caaaacgggc ccaaaaccgg ccttgcacag gaatgttggg ttaatcccct
     3781 tgaacatcgc gggaaggcac gcgacataga gcgccttcgc cagagaggtt ttgcatgata
     3841 gatctgttcg gacatgccgg ttccaatgcg cagggcaagt ttcacgtcga cccggtgacc
     3901 ggctcgctga tccccgtggt ggtcgagcaa tcgagccgcg gcgagcgcag cttcgacatc
     3961 tattcgcgcc tgctgcgcga gcggatcatc tttgtcaccg gccagatcga ggaccacatg
     4021 gcctcggtga tcgtggcgca gctgctgttc ctggaatcgg agaatccgag caaggacatc
     4081 agcatgtaca tcaactcgcc cggcggcgtc gtcacggcgg gcctcgccat ctacgacacg
     4141 atgaaataca tccgccccaa ggtttccacc gtgtgcatcg gccaggcctg ctcgatgggc
     4201 agcttcctgc tggccgccgg cgagccgggc atgcgcatcg cgctgccctc ggcgcggatc
     4261 atggtgcacc agccttcggg cggcgcccag ggcatggctt ccgacatcga gatccaggcg
     4321 cgcgagatcc tgcggatccg caagcggatg aacgatctct atgtgaagtt caccggccgc
     4381 agcctcgagg agatcgagaa tgcgatggat cgcgacacct tcctcgaagc cgatgaggcg
     4441 ctgaagttcg gcctgatcga caaggttttc gaaacccgcc ccaatgcgga cgagatttcc
     4501 ggcgaggcag gcaaggaata aggccccggg ccgggcgggc agcgcccttt ccggccgacg
     4561 ggattgatta tcgcgcccat ggggctagac ttcgcctgag tctgccccag gggtgcagtt
     4621 gggaaacaca atgacgaaac tgagcggatc ggacaccaag agcactctct actgcagctt
     4681 ctgcggcaaa tcgcagcatg aggtgcgcaa gctcattgcc gggcccacgg tgttcatctg
     4741 cgatgaatgc gtcgagctgt gcaacgacat catccgcgag gaatcgaaga gcggcatcgc
     4801 cggcaagaag gatggcggcg tgcccacccc gcgcgacatc ttcgagacgc tgaacgatta
     4861 cgtgatcggc caggaccgcg ccaagcgcgt gctctcggtg gcggtgcaca accactacaa
     4921 gcgcctgaag cacagcggca agggcggcga ggtggagctg tcgaagtcga acatcctgct
     4981 ggtgggcccc accggcagcg gcaagacgct gctggcccag accctggcca agacctttga
     5041 cgtgcccttc accatggccg acgccaccac gctgaccgag gccggctatg tgggcgagga
     5101 tgtggagaac atcatcctca agctgctgca ggccagcgac tataacgtcg agaaggccca
     5161 gcacggcatc gtctacatcg acgagatcga caagatcagc cgcaaggccg agaacccctc
     5221 gatcacccgc gatgtgagcg gcgagggcgt gcagcaggcc ctgctgaagc tgatggaagg
     5281 caccaccgct tccgtgccgc cgcagggcgg gcgcaagcat ccgcagcagg aattcctgca
     5341 ggtggacacg acgaacatcc tgttcatctg tggcggcgcc tttgcggggc tggaaaagat
     5401 catcgcggat cgcctgcaga agcgctcgat cggctttggc gcccatgtgg cagaccccga
     5461 caagcgcaag gtgggcgaac tgctgcagaa ggcggagccg gaagatctgc tcaagttcgg
     5521 cctgatcccc gaattcgttg gccgcctgcc ggtgatcgcc acgctggagg atctggatat
     5581 ccccgcgctg gtgcgcatcc tgcgcgagcc gaagaatgcg ctggtgaagc aatatgccaa
     5641 gctgttcgag ctggaggatg tggcgctgac cttcaccgag gaagcgctgg aagccatcgc
     5701 caagaaggcg atcgagcgca agaccggcgc gcgcggcctg cgttccatcg tcgaggggct
     5761 gctgctcgac acgatgttcg atgtgcccac cgaaaccggc attgccgaag tggtggtgga
     5821 caaggatgtg gtggaaggcc gcaaggagcc ggtgcgcgtg ctgaaggcca aggaagaggc
     5881 ggcctgaggc cagcgctttt cgcagcacat tgaaaggggc gggaaggctt gggctttccc
     5941 gcccctttcg cgttcagggg tagcgttcag ctcaccgggg ccgggccgcc ggcgggcagg
     6001 ggcggcgcgc ccaacgcctc gcccagctgg cggatgcggg cgacatgggc catcagcggg
     6061 gcccagtcgt cctgcgcgtc gatggcgg
//
LOCUS       DASRYE010000225        28372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_257671,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000225 DASRYE010000000
VERSION     DASRYE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 28372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28372
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257671"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFF98_11435"
     CDS             <1..205
                     /locus_tag="VFF98_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZU64288.1"
                     /translation="YCQRTGRNGIDNWDFYIAFNFFRLAAIFHGIKGRVLRGNAANAQ
                     AAARAQAFPRLARLARLAMERCA"
     gene            202..996
                     /locus_tag="VFF98_11440"
     CDS             202..996
                     /locus_tag="VFF98_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU64289.1"
                     /translation="MSGPVLLRHEGAVAVLTLNRPEAGNTVNMALALELEQAVNAIAD
                     DPAVRCVVLTGAGKLFCGGGDIGAFAEAGEEAPAFLHALASRLHRCVRRLAEMEKPLV
                     TLVNGPAAGAGLSLAILGDIVLAGASAHFTAAYGLVGLTPDGGMSWLLPRLVGLRRAQ
                     EILLTNRRIGAAEAQAIGLVTRVEEEEALLAEGLALAASLAAGPTGALGGARALLAGS
                     LQHDLASHLDTEAARIAEASATAEAREGIAAFLARRRPDFSERTPS"
     gene            993..2153
                     /locus_tag="VFF98_11445"
     CDS             993..2153
                     /locus_tag="VFF98_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZU64290.1"
                     /translation="MTQAYIVEAVRTAGGKRKGALADWHPADMGGAVLDALVARSGID
                     PAAIEDVILGCVTQAGEQGFAFARNAVLASGLPQSVPAVTVDRQCGSSQQCLQFAAQA
                     VMSGTQEVVIAAGAESMTRVPMFSNISYHAAQGVGIGPFSPRILERFGVSGFSQFEGA
                     EMIAAKYGFTREQLDRFALESHRRAHAASEAGAFAAEILPLQGAGGLHARDEGIRADA
                     TLEGIGAVKLLKEGGVISAANASQICDGASGVLVVSEGALQRHGLTPIARIVNMTVTA
                     GDPVIMLEEPINATQKALARAGLTIGDIDLYEVNEAFAPVPLAWAQALGADPARLNVN
                     GGAIALGHPLGATGTKLMTTLIHALRARGGRYGLQTVCEGGGIANVTIVEAL"
     gene            2153..3145
                     /locus_tag="VFF98_11450"
     CDS             2153..3145
                     /locus_tag="VFF98_11450"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZU64291.1"
                     /translation="MALKTRITELLGIAHPIVQGGMMWVGRAELASAVSNAGGLGILT
                     ALTQPTPDDLRREIDRCRAMTDRPFGVNLTILPSVSPPPYADYRRAIIESGVRIVETA
                     GHAPREHVEDFKSHGIIVLHKCTAVRHAVSAQKMGVDVISIDGFECAGHPGEDDIPGL
                     VLIPAAADQVTIPMLASGGIGDGRGLAAALALGAEGVNMGTRFCATREAPIHDNIKQF
                     LLANTERDTRLIFRRFHNTGRVAASPVSNRVVEISAGAEAVFEDIRPLVSGARGRAAL
                     ETGDLEAGLVWAGQVQGLIHDIPSCADLLARMVAEAQAIISRRLAGFLVAEPVQ"
     gene            3142..3918
                     /locus_tag="VFF98_11455"
     CDS             3142..3918
                     /locus_tag="VFF98_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU64292.1"
                     /translation="MSGASVLVSREGALTLITINRPEAHNALNPATQLELMAALDAFE
                     ADDGQWVAILTGAGEKAFCGGMDLKHQAAEGGLFTAPKGFGGITERPAMTKPVIAAVN
                     GIAMGGGFEMALACDIIVAAAHAVFALPEPRVGLAALAGGIQRLPAMIGLKQAMGLLL
                     TGRRVSAAEGLRLGFVNEVAEGDVLAAARAVAQEILACSPLAVRATKEAALRSLHLPA
                     EEAIAAQWQWPAMAAMLASEDAVEGPRAFAEKRRPAWRGR"
     gene            3903..5006
                     /locus_tag="VFF98_11460"
     CDS             3903..5006
                     /locus_tag="VFF98_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZU64293.1"
                     /translation="MAGALMAQKTQDAQGPLAGLRVIEFTSIGPGPHAAMLLADLGAD
                     VLRIDRAGGNGWPNPIVDRGRAAKAVDIRSEAGRDWCRAAAAHADVLIEGFRPGVMER
                     LGLGPDVLCAANPRLVYGRMTGWGQEGPLARSAGHDINYIALSGALAAMGRPGEPAAV
                     PLNLVGDFGGGSMFLVFGLLAALWERGRSGRGQVVDAAIIDGVASLMTMFTGLVPTGR
                     ISLDRADNLLGGAAPFYRCYLCADGREVSVGPLEPPFFAAMMAALGLGALAGAQYDTA
                     RWGELTGALAATFASQPQEHWASLFAGSDACVAPVLSAAEAPGHPHHLARATFVEAAG
                     LVQAAPAPRFSRTGGAIRPAGDADAMLAAWQAV"
     gene            5003..6145
                     /locus_tag="VFF98_11465"
     CDS             5003..6145
                     /locus_tag="VFF98_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64294.1"
                     /translation="MIPVAGRPLFGEEHALFRDSVRRFFAAHLLPHLDRWEREGIVER
                     AFWRRCGEMGLLCPTVPPAYGGPGLDFRCNAIVGEELFHAISSAGITLQSDITADYLL
                     HYGTEAQKRRWLPAMVRGEVITAIAMTEPGTGSDLKAIRTTARRQGTDYVLNGAKTYI
                     TNGQNADLVIVVAKTDPAAGAKGLSLFLVEATRTGFARGRNLDKIGQHSADTSELFFQ
                     DLRLPQENLLGAEGQGFACLMAQLPQERLSIAIAAQASAQRAFDEALAFTRDRRAFGQ
                     PVFDFQHTRFTLAGMQAKIQAGWAHVDWALACHLRKELSAAQASAAKLFHTELLWEVA
                     DAALQLHGGAGYMNEYPIARLWRDARVQRIYGGTSEIMKEVIGRAL"
     gene            6226..7347
                     /locus_tag="VFF98_11470"
     CDS             6226..7347
                     /locus_tag="VFF98_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64295.1"
                     /translation="MLIRHDDYPVHQTAEPLSYLASSERNMYARYWYNGYDFDGEFYI
                     GVAFAAYPNREVMDGAVSIVRKDGTQDSFRASRRLRGDRTDLFAGPLRHEIIEPMKTV
                     RVTVAPNDTGFTCDLTFHANTVAHEEPADHMRQGVRTVAQMKRFTQFGRWEGHVTVNG
                     RRQEVDIRSTYGTKDRSWGWRWTGEPEQGISRDMPEQFFWLWAPIHWADRCTHWGQHE
                     YADGRRWKEFAQIFRSFSARSGFDPLDEGGVEAALCGPHRLQFAPGSRLIRRAEIDTI
                     CRDGSTQTFVLEPLLRYHMYGTGYNHPEWGHGFYKGEEAHTLESWNIHEIDLNAPRFM
                     HVQQVVRATCGDEVGVGALEQYIVGPHARYGMTGFLDPL"
     gene            7363..8460
                     /locus_tag="VFF98_11475"
     CDS             7363..8460
                     /locus_tag="VFF98_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZU64296.1"
                     /translation="MRAAVFHNGRFTVEQRADPAPGPGQWLVRPIACGICGSDLGIRG
                     DAPHLCDVLHRAGFRTFMDPARPVVMGHEFVCELLEAGAGAGAGAAGLARGARLVALP
                     FLRDAAAGLQLLGYSNAYNGAFAEAMLIDAASALPVPDAVSDEAAALTEPLAVAVHAV
                     AAARPDGDCAFGVVGCGPVGLFVIARLKALGLGPVIAIEPDPVRRALAERMGADVVAA
                     GVDGSVDGRADGWWAGQGLPVGLSDAMAVDPAARARSRAVLFDCVGKPGLLMQIARAA
                     PVGATIIGVGTCKAEDRIEPAFLLQKGLCLHFVFAYAPAEFAEALAMIARAPDRLLPL
                     VSGQVGLGDLDSAFDALAGGGVVKLLVRPDL"
     gene            complement(8457..9515)
                     /locus_tag="VFF98_11480"
     CDS             complement(8457..9515)
                     /locus_tag="VFF98_11480"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZU64297.1"
                     /translation="MPMHVLVMGGNRYIGLQLVHELVAQGHKVTVLNSHPAPLPDGVV
                     RLHGQRHEAGVLEALLGPLRDRFDAVFDNTAYTPDHLRPMIALFRGRLRHFVFTSSIA
                     VYEMALAQPVDENAAVGRDAASALYGAYAAGKVACEELLAREHAAGGLPFTALRVAHT
                     CGPMSPAVTREPGTFKRLEEGRPLLIAGKSEAMVHFVHTRDAARALCAVLGKPQAAGQ
                     IYNVAGRQFSSIANYMRLMAETVGVAARIIDMPADLPAHMRSPIVHWLEASRGSMVFD
                     IGKIRRDLGWEPQLGLAESLADSYRWFCAGGRAHYTYDFAPDEEILAEIARRGGPGQP
                     PESARTVLRPQLNSLENL"
     gene            complement(9523..9921)
                     /locus_tag="VFF98_11485"
     CDS             complement(9523..9921)
                     /locus_tag="VFF98_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64298.1"
                     /translation="MTEPASPLHEARALLRQFQHGKARACHIRTPTLELFLSRDRACR
                     PQALPPAPDPDPAPAALPASPLAAPHLATVVELAGVGLAVAAGDVLARLRVLDRHSEL
                     RADSACVVAAHHLAPGALVQFGEALLSIQS"
     gene            complement(9914..11476)
                     /locus_tag="VFF98_11490"
     CDS             complement(9914..11476)
                     /locus_tag="VFF98_11490"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZU64299.1"
                     /translation="MAMTWTVRLDGADLPERALVGGKAWSIARMLALGLNVPPAFVVT
                     TRACAAYQNGDLPEGLEAEIAEGLAWLEAGSGRRFGAGPAPLLLSVRSGAAISMPGMM
                     DTVLNLGITDATEAALAAETGQPAFARDTHRRFLELYAGIVLKAPAHLPPEGTPAQWR
                     AAVALAAGQHLPEDPRAQLLAAVRAVFDSWNSRRARKYRQHNAIPDDLGTAVTIQAMV
                     FGNLDADSGTGVLFSRNPLTGARAPFGEYLPQAQGEDVVSGKFTPLRLEAMHERVGAA
                     HDLLLAASERLEQDSRDMQDIEFTVQKGRLYLLQARAAKRAPAAAVRCAVEMAEEGLI
                     SRAEALARVSPEQAAELLRPRLAEGATAGLAPIATGEGACPGVASGRVVSSPDEAEAR
                     AAAGEAVILARETTSPDDIHGMIGARAIITAQGGATSHAAVVSRALGRPCVVGCGAGV
                     LALAGQVVTVDGSGAVYAGALPLVVPGAADHPFLGRLLAWAEEAGEAKFAARPDARPD
                     AASLKTLGAVHD"
     gene            complement(11476..12078)
                     /locus_tag="VFF98_11495"
     CDS             complement(11476..12078)
                     /locus_tag="VFF98_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64300.1"
                     /translation="METRDLALHGLAIKRHAGPAAVAELTGLDAEQAAGELARAVATG
                     RAVEARGAFTLTPLARVALQASYERLCAALRADAGFAAAYHGFERINPELKQVITDWQ
                     TIDLHGERRANDHTDQAWDDKVIDRLSALHERAEAVLAGLARALPRMAIYGRKLEAAL
                     DRAEGGAIEWVSDIHRDSYHTVWFELHEDLLRLMGRERVE"
     gene            complement(12078..12842)
                     /locus_tag="VFF98_11500"
     CDS             complement(12078..12842)
                     /locus_tag="VFF98_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HZU64301.1"
                     /translation="MAGLPIDEMRAAPPPASPIVEATLALLHQAVRRKLAAHVAQGGA
                     GFDDFVFAAPGSAEDCAISHADVAAVEAALLASGHRFDWSAAISLAERPEAYKGGTGG
                     ADAVVFEHPGAITGAPDAQGRTPCGQGDNVVRHEADITGTARYIRSNERVLAWLAEGV
                     PPGTIAIIDDSGGTLTAPILEQFAGVLCAGGTVRSHLGILSREYGIPCFMNSKLSGIA
                     DGDTVCMEASAPPRTTEDYQSGMERTGRVWKLAGEA"
     gene            13077..13868
                     /gene="surE"
                     /locus_tag="VFF98_11505"
     CDS             13077..13868
                     /gene="surE"
                     /locus_tag="VFF98_11505"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZU64302.1"
                     /translation="MNDDGIDGPGIRLLEEIARGFTDDVWVVAPDEERSGAGQAISIS
                     HPIRYTQRDERHFAVRGTPTDCALLGINTFLEGRKPDILFSGINAGPNLAEDVHYSGT
                     CAAAREGALLGIPSFALSQMRAYGTPTHWEASATHLPGVVAQLLALPWAPGSFANINV
                     PNLPPAEITGIRITRQGQRRPGGFLPVRRVDERTVPYFWVKICPNPPELVAGTDLHAV
                     AHGAISITPMQLDLTRHQAIAGLEAVFGASDGAPGPLLAAADACI"
     gene            13880..14593
                     /locus_tag="VFF98_11510"
     CDS             13880..14593
                     /locus_tag="VFF98_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU64303.1"
                     /translation="MTSDMPFSIADSDVAPASPRDTIQQVTEGLIYCIRAGQMVPGQH
                     LVEADLTQRFGVSRGSLREGLKQLAADGIVTLSRFRGAFIASLDRKGVSDLLDVLEPL
                     CMLAARLAAQACQQADAHATLHEVAALLERHGENGGRAAYLENRRRFYDLLIEMGGNS
                     ELGRVIPLARTDLFRAQFDTVQTKEQHRRHARGYARIAEAVAQNDPAKAERAVRRHFA
                     GTRATLDVLPDQAFAVALR"
     gene            complement(14623..15507)
                     /locus_tag="VFF98_11515"
     CDS             complement(14623..15507)
                     /locus_tag="VFF98_11515"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HZU64304.1"
                     /translation="MRKGWMICCAALLLAMPAGLVAQLPVPPAPAAHQRLLPLQGGRN
                     FRDLGGYRTADGHHVKWGLLYRSGAMHGLTGADYALLESRGIRTVCDLRDARERAAEP
                     ADWPPAHAPRQLFDAYTLDVKALMPPGDPQGWRVGQVRAAMIASYPRLLVLLNGQYRR
                     MFARLLAGEAPLAFNCTAGKDRTGIAAALILTALGVPRHTIVADYMLSNRYLDLAVMR
                     GSARAPANMASGKSALGMLASLPPPVFHALMAADPAYIGAALAVMERHRGGPAGYLAQ
                     EMGLSAQDIRQLRALYLE"
     gene            complement(15531..16754)
                     /locus_tag="VFF98_11520"
     CDS             complement(15531..16754)
                     /locus_tag="VFF98_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZU64305.1"
                     /translation="MRAVDIIGVGMTPFGRHLDLSYSALAQAAVKEALADAGIAPSAL
                     DTSIFSSVVQGFVAGEMSIPGEYALRPLGIAGVRAFHVEAACASGTMALHLAYDLIRA
                     GLADVVLTVGVEKLYTQDRAKKFAVFQQPLDIGVAEAYLARTQGRLAPVPAGQEAPGP
                     NVLMQAYAAQARLHMATYGSTPAHFAAISAKNHAHSVHNPLAQYRHALSLEEVLAAPT
                     VAWPLTVPMCAPISDGAAAAVLCAADLTGRLGAGAPVRVRACRSLSGHDRDPADYAGH
                     VSAKVAALAYADAGLGPQDLDLAEVHDASSTGEAIQIEALGLAPPGEAGARAMAGEWT
                     LGGRLPVNVSGGLVSKGHPLGATGLAQIHELVTQLRGRAGARQVAGARVALAENSGGF
                     YGVEDALSSVTVLGG"
     gene            complement(16754..18310)
                     /locus_tag="VFF98_11525"
     CDS             complement(16754..18310)
                     /locus_tag="VFF98_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU64306.1"
                     /translation="MRMIDFFDRGARNAPSQPCMVSAGRSITYAQLRARSIRIAHALI
                     ADGFAAGAHGAVLSGNDPAAFEAILGLLRADGVWTMANARASEDENAYLLDLLDVDTL
                     FLHTEVAERIARFRRDCPKIRRYVTIDAPHAEADAFLGDWLARPNDAEVPRRRHGAPD
                     ELAFLANTGGTTGRPRGVMLTNLVWQSLVSSLVASMPMKAPPVNLVAAPMTHAAGPLA
                     LASMALSGTVVVLPRFEAGAVLEAIERHRVTYMFLPPTAIYMLLDHPEVRRRDLASLE
                     YVSYAGAPMSAERLKEAIALFGPVMNASFGQTEAPMCVTAMPPHQHLNAAGELAHPGS
                     CGQANLLSQVEIMDEEGRLLPPGERGEIVVRGPLVMAGYYRNPEATAEVSRFGWHHTG
                     DIGYRDAEGWFFIVDRKKDMIISGGFNVYPAEVEQALQAHPAVQDCAVFGIPDDKWGE
                     QVTAVVECRAGIAPPPPEELIAFARARLGAVKTPKQLTYTEALPRSNAGKVLRAALRQ
                     PYWNQRERTI"
     gene            complement(18324..19778)
                     /locus_tag="VFF98_11530"
     CDS             complement(18324..19778)
                     /locus_tag="VFF98_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HZU64307.1"
                     /translation="MAHIRFLDETCRDGQQSLWGMRMQAGQAYPVLPTIDRTGYSTIS
                     LAGSSLFEVLVRHCQEDPWAGFDLMMSQIRQTPVRGGIRSNGSVTFGFTPDCLMDLWM
                     ERLCVHGVRSWWIYDVLFNIDKMARLAKLSKKYDCKVAGTMLFTLSPVHDDAFWADKA
                     AKLAAIPEIDSLLLYDTGGVLDRERIQTLVPAIVANAGGKPVEMHSNNMLGQSAKAYC
                     DAVECGVSVLHTAARSMANGPSIPSIDVMVRNMEALGHTHDIDTSLLPPVSDHFRRVA
                     KAAGYLHDQHYEYDVTSLTTQIPGGMMGTLRNQLIQQNMLDKLPVVLEEVATVRRELG
                     YPGMATPFAQLVGTLAVLNIVTGKRYSAVPDEVIQYAAGYYGEPPAPIDPEALDRIMA
                     APRARDIVNNPPPQPDLAELKRQHGTDDEDELILRALVPAADIARMRKSGPPARDFPM
                     LSSPELAQVEKLMRVATAPLVRLKTEGLEVSIRR"
     gene            complement(19791..21149)
                     /locus_tag="VFF98_11535"
     CDS             complement(19791..21149)
                     /locus_tag="VFF98_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZU64308.1"
                     /translation="MALQRLFIANRGEIALRIAHAAKALGIETVIGVSDADRDSAAAR
                     AATRVVVLGPSPAARSYLDPELIVHAARETGCDGLHPGYGFLSERAVLPRLCAAHGIA
                     FVGPDAETIEALGDKIRARAMARAAKVPLVPGSDSIASAADAVRVAEEIGYPVLLKAS
                     AGGGGRGMVIAHSGAEVEAGFARASAEAESAFGDGTLFMERFVPRARHVEVQLMGDGT
                     GKVLHFGERDCSVQRRYQKLIEEAPSAAMPEALRRQLHASAVALAASVRYKGAATAEF
                     LYDVDRQEIYFIEVNARIQVEHPVSEAVTGADLVQWQLRIAGGETLSIEQADIAVAGH
                     AIECRINAEDWQRGFIPSPGTISRWAPPAGTHIRLDTHMGEGATIPPYYDSMIGKLIV
                     SGATRAEAIDRLEAALADFAVAGVPTTIGLHRRIIAHPDFRANAIHTRWLEQVLLPSL
                     DQ"
     gene            complement(21153..21659)
                     /locus_tag="VFF98_11540"
     CDS             complement(21153..21659)
                     /locus_tag="VFF98_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZU64309.1"
                     /translation="MAQPRDNEDLEALIRQFQASDLEELHIKAAGLELFLSRDPQASA
                     PWDGAPATPQPAAAPAPAAPAPATPTQPAPAAPAPAPAPAETDGLTLVRAPYLGTFYR
                     APKPGEPVFCEKGDTVEAGADLCLVEVMKLFTAVRAESKGIVRHIYASDGDMVSEGQV
                     LFALEPVA"
     gene            complement(21698..23275)
                     /locus_tag="VFF98_11545"
     CDS             complement(21698..23275)
                     /locus_tag="VFF98_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64310.1"
                     /translation="MSSLAEQGAGQSGLTYARILEANNAIQTIGDDTYWLCVTRTVQE
                     SKLFPVPAYMLLSYLMCFYRYPALLRKIEAAMPAEEIGDRAREMGAKFGNLPGWGMVT
                     FYLLGREMLINFGMLRPEDAAEDTCYVLDFWRRFKLAQQREDGHLNAREFGQRVQLLP
                     ERRVQVFHADAHRCAPGDRLHKAAQGFLAAVSQYGFMISCESRVTLNNSGPYRLADNR
                     ELIIRDFTDLAAGDYPWLDGVGDPIAYNNLTVTMEVADCHFYLMDDWGSFESRPEFTA
                     DKLVGVGLYTSDVLSDGYMPVGMGSAEELAATFETLTEQVRAAIAALWKRVAGWSRDE
                     MMDAGALVYFSIIKDFAHVAGVYEQDDWMTIDPRADRFRPLLNDEFGRDFLGELVGHV
                     DLPSQHLADHMMMQHNNLPRRYLSHIPFSVLDGEGAAQKPGILPGTSRHAAKRDSYTT
                     TQGPLPLAEYNARARAHRPAQMDPLYRGLSDTWLKWHPADPRADALYRLEQRRSRLLK
                     DRGIGLAAADIEALRRG"
     gene            23711..24433
                     /locus_tag="VFF98_11550"
     CDS             23711..24433
                     /locus_tag="VFF98_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU64311.1"
                     /translation="MRLNRAIAEASHRLVIDCALDTASIFFCDHSTGDGSLSYLHHVN
                     ITSEAQHAYRHHGLFRHDPFVALTRGDASGRGPGFVAWGDRRLEAATHSARDYHSFLG
                     QHGVAVAGALGRRVSSQVSLIIGAHRRPRGQHCGEVPMGLLEQRINALCEMVIDGLFE
                     ELLGDAAGRQVLAEAGSPPLLAERESQITAMICQGLRNKEIAWRLGISEYTVENHLRR
                     IYRKLGIHNRAALVARYSGRCH"
     gene            complement(24452..24862)
                     /locus_tag="VFF98_11555"
     CDS             complement(24452..24862)
                     /locus_tag="VFF98_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64312.1"
                     /translation="MKPDVSLAAAELARRLREELQPELTGFRANTAGMGAAMLDMIAD
                     AWDGAAARLVAENRALRALLQRGGDALAPEAAEEDLRISALTAANDRLRGQLIALHAR
                     IEQAEGPAARDLERAIWAFLRASVAARQIRSANF"
     gene            complement(24859..25899)
                     /locus_tag="VFF98_11560"
     CDS             complement(24859..25899)
                     /locus_tag="VFF98_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZU64313.1"
                     /translation="MADITTDQLTAYLAARMPTWHDLAIEGLSRLPMGASRETYRFDL
                     IHTDAAGTRQRLQLILRRDPPASNVDSDRAHEFAAYRAIYGHGIPVPRMLLLEEDPAP
                     LGGAISLAEDLRGFHNSEYLFQQPAWAPLLPHVARQMWGTMGRLAAVPVERLDLSFTR
                     PATREGTAAAELAYWVASLDRHDIGPEPIIRAAIRRLERWQPVAAKLALVHGDFRAGN
                     FLYDDAGNLQAVLDWEMAHCGDPLEDLAWSLTRSFCFGKDARRSGLAPHAEAVRLWEA
                     ASGLEADPQALWWWELFTCVKAQAIWNAAAHVWASGAGREVIHAYAAWWLRNTQDKAA
                     LELMGQLTETLP"
     gene            complement(25969..28230)
                     /locus_tag="VFF98_11565"
     CDS             complement(25969..28230)
                     /locus_tag="VFF98_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64314.1"
                     /translation="MSVSMKSLLLCATASLAWGAPALAENADAPTTSAPAGNGEIIVT
                     AQRRSESLMKVPIAMSALSQEDLVKRGITNTSALAGEVPSLQVNSPYGESQPNFTLRG
                     IGVANEHNPNQASPVGIYFDDAYIAARAAHGLNLFDLERVEVLRGPQGTLYGRNTTGG
                     AINFISRKPGLSGDNGNIQLGYGSYNTATAEGAVETTLKDGVAGLRVAFNYAHGDGYI
                     HNIALGQPNANSTDSVAGRAILRVKPTDRLDITLKGTIGRSNPTQASVYNLGTGADGY
                     NPVLGTSRAQKGLGFFETDSQRLGHSLVYAYGAELTVKYQLSDAVALMSLTSYDHVRQ
                     AFTQEGTGLESPLYQQPLDTLYGNRFTMFNQELRASYSAGHTNAQAGLYYGYDKDASN
                     SYYWLLDGAAMDHQIYDQIRKSYALFAQADQKFGDHLGVTVGLRYTWDRLRYQNYTSY
                     LTPSSLYTGTPDTTIFPFSQGTYVFGAYNAATGGYTSGPTIPLSSQALTGRLAINYTF
                     TGGHMLYASYGRGYRSAAYCGQCFLGPTITTTRPESVDAYEIGAKGRFADGHLSLAVA
                     AFLMNYRNQQINETEGAQVLLDNVPKSQIKGAEIDGSWTPLPGLKLALNGSYIDARYK
                     SAQLLLGSVAGNRLPYAPEWQFSGHLDWLVMELGKGQIHFIPSVVYTDRVYFTPYNAL
                     QGNQNLTQAPNAKVNAQLSYDTQRYTLRLWTNNLFNAKTFADGLDLRASFGFDYLVQA
                     PPRTFGLSANYRF"
BASE COUNT         4349 a         9345 c        10033 g         4645 t
ORIGIN      
        1 ctattgccag cgcaccggcc gcaatggcat cgacaactgg gatttctaca tcgccttcaa
       61 cttcttccgc ctggccgcca tcttccacgg catcaaaggc cgcgtgctgc gcggcaacgc
      121 cgccaacgcc caggccgccg ccagagcaca agccttcccc cgcctcgcca gactggcaag
      181 gctggcgatg gagcggtgcg catgagcggg cccgttctgc tgcgccatga aggcgccgtg
      241 gcagtgctga cgctgaaccg gcccgaggcg ggcaatacgg tgaacatggc gctggcgctg
      301 gaactggagc aggcggtgaa cgccatcgcc gatgatccgg cggtgcgctg tgtggtgctc
      361 acgggggcgg gcaagctgtt ctgcggcggc ggcgatattg gcgcttttgc cgaagcgggg
      421 gaggaggcgc cggctttcct gcatgcgctg gccagcaggt tgcaccgctg cgtgcgccgg
      481 ctggcggaga tggagaagcc gctggtcacg ctggtgaacg gcccggcggc gggtgcgggg
      541 ctgagcctgg cgatcctggg cgatatcgtg ctggcggggg cctctgcgca tttcaccgcg
      601 gcctatgggc tggtgggcct cacgcctgat gggggcatga gctggctgct gccgcggctg
      661 gtgggcctgc gccgcgcgca ggagatcctg ctgaccaacc gccgcattgg cgcggccgaa
      721 gcgcaggcga tcggccttgt cacccgcgtg gaggaggagg aggcgctgct ggccgaaggg
      781 ctggcgctgg cggcatcgct ggcggccggc cccaccggtg cgctgggcgg cgcgcgggcg
      841 cttctggccg gcagcctgca gcacgatctg gccagccatc tcgacaccga ggccgcccgc
      901 attgccgagg ccagcgccac cgccgaggcg cgcgaaggca ttgccgcctt cctcgcccgc
      961 cgcagacccg atttttccga aaggaccccg tcatgaccca agcctatatc gtagaggccg
     1021 tgcgcaccgc gggcggcaag cgcaagggcg cgctggccga ttggcacccg gccgacatgg
     1081 gcggcgccgt gctcgatgcg ctggtggcgc gcagcggcat cgatccggcc gccatcgagg
     1141 atgtgatcct cggctgtgtc acccaggctg gcgagcaggg ctttgccttt gcccgcaatg
     1201 ccgtgctggc ctcgggcctg ccgcaaagcg tgccggccgt cacggtggac cgccagtgcg
     1261 gatcgagcca gcaatgcctg caatttgccg cccaagcggt gatgagcggc acgcaggagg
     1321 tcgtcatcgc cgccggcgct gaaagcatga cccgcgtgcc gatgttctcg aacatcagct
     1381 atcatgcggc acaaggcgtt ggcatcggcc cgttctcgcc gcgcattctt gagcgtttcg
     1441 gcgtcagcgg cttcagccag ttcgagggcg ccgagatgat cgctgccaaa tatggcttca
     1501 cccgcgagca gctcgaccgc tttgccctgg aaagccaccg ccgcgcccat gccgccagcg
     1561 aggcgggcgc ctttgccgcc gagatcctgc cgctgcaggg ggccggcggg ctgcatgcgc
     1621 gcgatgaggg catccgcgcc gatgccacgc tggaagggat tggcgcggtc aagctgctga
     1681 aggagggcgg ggtgatctcg gcggccaatg ccagccagat ctgcgatggc gcctcgggcg
     1741 tgctggtggt gagcgagggg gcgctgcagc gccatggcct cacccccatc gcccgcatcg
     1801 tcaacatgac ggtgacggcc ggcgatccgg tgatcatgct ggaagagccg atcaacgcca
     1861 cgcaaaaggc cctggcccgc gccggcctga cgattggcga catcgacctt tacgaagtga
     1921 acgaggcctt tgccccggtg ccgctggcct gggcgcaggc gctgggggca gatccggccc
     1981 ggctcaacgt gaacggcggg gcgattgcgc tgggccatcc gctgggcgcc accggcacca
     2041 agctgatgac cacgctgatc catgccctgc gcgcgcgcgg cggccgttat ggtctgcaaa
     2101 ccgtgtgcga gggcggcggc attgccaatg tgacgattgt ggaggcgctc tgatggccct
     2161 gaagacacgc atcaccgaat tgctgggcat tgcccatccg atcgtgcagg gcggcatgat
     2221 gtgggtgggc cgggcggaac tggccagcgc cgtttccaat gccggcgggc ttggcatact
     2281 caccgcgctg acccagccga cgcccgatga cctgcgccgc gagatcgacc gctgccgggc
     2341 gatgacggac aggccctttg gcgtcaacct caccatcctg ccttcggtgt cgccgccgcc
     2401 ctatgcggat tatcgccgcg ccatcatcga gagcggcgtg cgcattgtcg aaaccgccgg
     2461 ccatgccccg cgcgaacatg tcgaggattt caagagccac ggtatcattg tgctgcacaa
     2521 atgcaccgcg gtgcgccatg ccgtctcggc gcagaagatg ggcgtcgatg tcatcagcat
     2581 cgacgggttc gaatgcgccg gccacccggg cgaggatgat attcccggcc tcgtcctcat
     2641 cccggccgcc gccgatcagg tgacgatccc catgctggcc agcggcggca tcggcgatgg
     2701 gcgcgggctg gccgcggcgc tggcgctggg ggccgaaggg gtgaacatgg gcacccgctt
     2761 ctgcgccacc cgcgaggcgc ccatccacga caacatcaag cagttcctcc tggccaatac
     2821 cgagcgcgac acgcggctga tcttccgccg cttccacaac accgggcgcg tggcggccag
     2881 cccggtgtcg aacagggtgg tggagatttc ggccggggcc gaggcggtgt tcgaggatat
     2941 ccgcccgctg gtctcgggcg ccagggggcg cgcggcgctg gaaaccggcg atctggaagc
     3001 gggcctcgtc tgggccgggc aggtgcaggg gttgatccac gacattccca gctgcgccga
     3061 cctgctggcg cgcatggtgg ccgaggcgca ggcgatcatc agccgccgcc tggccggctt
     3121 cctcgtggca gagccggtgc aatgagcggc gcctccgtgc tggtgagccg cgaaggcgcg
     3181 ctcacgctca tcaccatcaa ccggcccgag gcgcataatg cgctgaaccc tgccacccag
     3241 ctggagctga tggccgcgct cgatgctttc gaggcggatg acgggcaatg ggtggcgatc
     3301 ctcaccggcg ctggcgaaaa ggccttttgc ggcggcatgg atctgaagca ccaggccgcc
     3361 gagggcgggc tgttcaccgc gcccaagggc tttggcggca tcactgaacg cccggcgatg
     3421 accaagcccg tgatcgccgc ggtgaacggc attgccatgg gcggcggctt cgagatggcc
     3481 ctggcctgcg atatcatcgt ggctgcggcc catgccgtgt ttgccctgcc agagccgcgc
     3541 gtggggctgg cggcgctggc tggcggcatc cagcggctgc cggcgatgat cggcctcaag
     3601 caggcgatgg gcctgttgct gacggggcgg cgggtgtcgg cggcagaagg gctgcggctg
     3661 ggctttgtca acgaggtggc cgagggcgat gtgctggccg ctgcccgggc ggtggcgcag
     3721 gagatccttg cctgcagccc gctggccgtg cgcgccacca aggaagcggc gctgcgctcg
     3781 ctgcatctgc cggccgagga ggcgatcgcc gcgcagtggc aatggccggc gatggcggcc
     3841 atgctggctt ccgaagatgc cgtggaaggc ccgcgcgcct ttgccgaaaa gcgccgtccg
     3901 gcgtggcggg ggcgctgatg gcgcagaaaa cgcaagatgc gcagggcccg ctggccggcc
     3961 tgcgggtgat cgagttcact tccatcgggc cggggcccca tgccgccatg ctgctggcgg
     4021 atctgggcgc cgatgtgctg cggatagacc gcgccggcgg caatggctgg cccaacccga
     4081 tcgtcgatcg cgggcgggcg gccaaggcgg tggatatccg ctctgaagcg gggcgtgact
     4141 ggtgccgggc ggccgctgcc catgccgatg tgctgatcga gggcttccgc cccggcgtga
     4201 tggagcggct gggccttggg cccgatgtgc tgtgcgcggc caatccgcgc ctcgtttacg
     4261 ggcggatgac gggctggggg caggaaggcc cgctggcccg cagcgccggg catgatatca
     4321 actacatcgc cctgtcgggc gcgctggcgg ccatgggccg gccgggcgag ccggcggcgg
     4381 tgccgctcaa tctggtgggc gattttggcg gcggctcgat gttcctcgtc ttcggtctgc
     4441 tggccgcgct gtgggagcgg gggcgttccg ggcgcgggca ggtggtggat gcggcgatca
     4501 tcgatggcgt ggcctcgctg atgacgatgt tcacagggct ggtgccaaca gggcgcatct
     4561 cgctcgatcg ggcggataat ctgctgggcg gggcggcgcc cttctatcgc tgctatctgt
     4621 gcgccgatgg gcgggaagtg tcggtggggc cgctggagcc gccctttttc gccgcgatga
     4681 tggcggcgct ggggctgggc gcgctggccg gcgcgcaata tgacaccgcc cgctggggcg
     4741 agctgaccgg cgcgctggcg gccactttcg ccagccagcc gcaggagcac tgggccagcc
     4801 tgtttgccgg cagcgatgcc tgcgtggccc cggtgctgag cgccgccgaa gcgcccggcc
     4861 atccccatca cctggcccgc gccacctttg tggaagcggc cgggctggtg caggccgcgc
     4921 ccgcgccgcg cttctcgcgc acggggggcg ccatccggcc ggcgggcgat gccgatgcca
     4981 tgctggccgc ctggcaggcc gtatgatccc tgtcgccggc cgcccgctct tcggcgagga
     5041 acatgcgctg tttcgcgaca gtgtgcgccg tttcttcgcc gcccacctcc tgccccatct
     5101 cgaccggtgg gagcgcgagg ggatcgttga gcgcgccttc tggcggcgct gcggcgagat
     5161 gggcctgctg tgccccaccg tgccgccggc ctatggcggg ccggggctgg atttccgctg
     5221 caacgcgatt gtcggcgaag agctgttcca tgccatttcc tcggccggca tcacgctgca
     5281 atcggatatc accgccgatt acctcctcca ttatggcaca gaggcgcaga agcggcgctg
     5341 gctgccggcg atggtccgcg gcgaggtgat caccgccatc gccatgaccg agccgggcac
     5401 cggatccgac ctcaaggcca tccgcaccac cgcccgccgg cagggcacag attatgtgct
     5461 gaacggcgcc aagacctata tcaccaacgg gcagaacgcc gatctggtga tcgtggtggc
     5521 caaaaccgat ccggcggccg gcgccaaagg tctctcgctg tttctggtgg aagcgacgcg
     5581 caccggcttt gcccgcggcc gcaacctcga caagatcggc cagcacagcg ccgatacctc
     5641 cgagctgttc ttccaggatc tgcgcctgcc gcaggagaac ctgctgggcg cagaagggca
     5701 gggctttgcc tgcctgatgg cgcaattgcc gcaggaacgg ctgagcattg ccattgccgc
     5761 ccaggccagc gcccagcgcg ccttcgacga ggcgctggcc ttcactcgcg atcggcgggc
     5821 gtttggccag ccggtgttcg atttccagca cacccgcttc accctggcgg gcatgcaggc
     5881 gaagatccag gccggctggg cgcatgtgga ttgggcgctg gcctgtcatt tgcgcaagga
     5941 gctgagcgcg gcgcaggcct cggcggccaa gctgttccac accgaactgc tgtgggaagt
     6001 ggctgatgcg gcgctgcagc tgcatggcgg cgcgggctat atgaacgaat accccatcgc
     6061 ccgcctgtgg cgcgatgcgc gggtgcagcg gatctatggc ggcacgtctg aaatcatgaa
     6121 ggaagtgatc ggccgggcgc tctagctaac gcgtttccgt ctcttggacg ggcccgggcg
     6181 ccgggcgaca aggcgggcag acctctgctt ttccgggaca agcccatgct gatccgccac
     6241 gacgactacc ccgttcacca gaccgccgag ccgctctcct atctcgcttc ctcagagcgc
     6301 aacatgtatg cccgctattg gtataacggc tatgatttcg atggcgaatt ctacatcggc
     6361 gtggcctttg ccgcctatcc caaccgggaa gtgatggatg gcgcggtgtc catcgtgcgc
     6421 aaggatggca cgcaggattc cttccgcgcc tcgcgccggc tgcgcggcga tcgcaccgat
     6481 ctgtttgccg gccccctgcg ccacgagatc atcgagccga tgaagaccgt gcgcgtgacc
     6541 gtggccccca acgacaccgg tttcacctgc gatctcacct tccacgccaa caccgtggcg
     6601 catgaggagc cggccgacca tatgcgccag ggcgtgcgca ccgtggcgca gatgaagcgc
     6661 ttcacccagt ttggccggtg ggaagggcat gtcaccgtca acgggcggcg gcaggaggtg
     6721 gatatccgca gcacctatgg caccaaggac cgctcctggg gttggcgctg gacgggcgag
     6781 cccgagcagg gcatcagccg cgacatgccc gagcagttct tctggctgtg ggcgccgatc
     6841 cactgggcgg atcgctgcac ccattggggc cagcacgaat atgcggatgg ccggcgctgg
     6901 aaggaatttg cccagatctt ccgcagcttt tcggcccgaa gcggcttcga tccgctggac
     6961 gagggcgggg tggaggcggc cctgtgcggc ccgcaccgcc tgcaattcgc gcccggcagc
     7021 cggctgatcc gccgcgccga gatcgacacc atctgtcgcg atggcagcac gcagaccttc
     7081 gtgctggagc cgctgctgcg ctatcacatg tatggcaccg gctataacca cccggaatgg
     7141 ggccacggct tctacaaggg cgaggaagcc cacacgctgg aaagctggaa catccacgag
     7201 atcgacctca acgccccccg cttcatgcat gtgcagcagg tggtgcgcgc cacttgcggc
     7261 gatgaggtgg gcgtgggcgc gctggagcaa tatatcgtcg gcccgcatgc gcgctacggg
     7321 atgaccggct ttctcgatcc gctctgaacc gggaggcagg atatgcgcgc ggcggtattt
     7381 cacaacgggc gtttcacggt ggagcagcgg gccgatcctg cccccggccc cgggcaatgg
     7441 ctggtgcggc cgattgcctg cggcatttgc ggcagcgatc tgggcatccg cggggatgcg
     7501 ccgcatctgt gcgatgtgct gcaccgggcg ggttttcgca ccttcatgga tccggcgcgg
     7561 ccggtggtga tggggcacga attcgtctgc gaactgctgg aggcgggcgc gggcgcgggc
     7621 gcgggggctg cgggcctggc ccgcggggcc cggctggtgg cgctgccttt cctgcgcgat
     7681 gcggcggccg ggctgcagct gctgggctat tccaacgcct ataacggcgc ctttgccgag
     7741 gccatgctga tcgatgccgc cagcgccttg ccggtgcccg atgccgtgag cgatgaggcc
     7801 gctgcgctga ccgagccgct ggccgtggcg gtgcatgcgg tggccgcggc ccggcccgat
     7861 ggcgattgcg cctttggcgt ggtgggctgc ggccctgtgg ggctgttcgt catcgctcgg
     7921 ctgaaggcgc tggggctggg cccggtgatc gccatcgagc ctgatcccgt gcgccgggcg
     7981 ctggccgaac ggatgggcgc cgatgtggtg gccgccggcg ttgatggcag cgtcgatggc
     8041 cgggccgatg gctggtgggc ggggcagggg ctgccggtgg gcctgtccga tgcgatggcg
     8101 gtggacccgg cagcgcgggc gcgttcgcgc gcggtgctgt tcgattgcgt gggcaagccg
     8161 ggcctgttga tgcagatcgc gcgggcagcg ccggtggggg caacgatcat cggcgttggc
     8221 acctgcaagg cggaggaccg gatcgagccg gccttcctgc tgcaaaaggg cctgtgcctc
     8281 catttcgtct tcgcctatgc gcccgccgaa tttgccgagg cgctggcgat gatcgcccgc
     8341 gcgcccgatc ggctgttgcc gctggtgagc gggcaggtgg ggctgggcga tctggacagc
     8401 gctttcgacg ccttggccgg cggcggcgtg gtgaagctgc tggtgcgccc cgatctctag
     8461 aggttttcca ggctgttcag ctgggggcgc agcacggtgc gggcgctttc cggcggttgg
     8521 ccagggcccc cgcgccgggc gatttcggcc aggatctcct cgtcgggcgc gaaatcatag
     8581 gtataatgcg cgcgcccgcc ggcgcagaac cagcgatagg aatcggccag gctctcggcc
     8641 aggcccagct gcggctccca gcccagatcg cggcggatct tgccgatatc gaacaccatc
     8701 gagccgcggc tcgcctccag ccagtggacg attggcgagc gcatgtgggc gggcaggtcg
     8761 gccggcatgt cgatgatccg ggcggccacg cccacggtct cggccatcag gcgcatgtag
     8821 ttggcgatgc tggaaaactg ccgccccgcc acattataga tctgccccgc cgcctgcggc
     8881 ttgcccagca ccgcgcacag cgcgcgggcg gcatcgcggg tgtggacgaa atggaccatc
     8941 gcctcgctct tgccggcgat cagcagcggg cggccttcct ccagccgctt gaaagtgccc
     9001 ggctcgcgcg tcacggcagg gctcatcggg ccgcaggtgt gggccacgcg cagggccgtg
     9061 aagggcaggc caccggcggc atgttcgcgc gccagcagtt cctcgcaagc caccttgccg
     9121 gcggcatagg cgccatacag cgcgctggcg gcatcacggc ccacggcggc gttttcgtcc
     9181 accggctggg ccagcgccat ttcatacacc gcgatggagg aggtgaagac gaaatgccgc
     9241 agccggccgc ggaacagcgc gatcatcggg cgcagatgat cgggcgtgta ggcggtattg
     9301 tcgaacacgg catcgaaacg gtcgcgcaag gggcccagca gggcttccag cacgccggcc
     9361 tcgtggcgct ggccatgcag gcgcacaacg ccatcgggca gcggcgccgg atggctgttc
     9421 agcaccgtca ccttgtggcc ctgcgctacc agctcgtgca ccagttgcag cccgatatag
     9481 cggttgccgc ccatcaccag cacatgcatc ggcatttccc tttcagcttt ggatgctcaa
     9541 cagcgcttcg ccgaactgca ccagcgcgcc gggcgccaga tgatgggcag caaccacaca
     9601 ggcgctgtct gcgcgcagtt cgctgtgccg gtcgagcacg cgcagccgcg ccagcacatc
     9661 gccggcggcc accgccagcc ccacgccggc cagctccacc accgttgcca gatggggcgc
     9721 ggccagcggg ctggctggca aggccgctgg cgccggatcc ggatccgggg ccgggggcag
     9781 ggcctgcggg cggcaggccc tgtcgcgtga gaggaacagc tccagcgtgg gagtgcggat
     9841 atggcaggcg cgcgccttgc catgctggaa ctggcgcagc agcgcgcgcg cctcatgcag
     9901 cggcgaggca ggctcagtca tgcacggctc ccagcgtttt cagcgatgcg gcatcggggc
     9961 gggcatcggg gcgggcggcg aatttggcct cgccggcctc ctctgcccag gccagcaagc
    10021 ggccgaggaa ggggtgatcg gcagcgccgg gcaccaccag cggcaaggca ccggcataga
    10081 ccgcccctga gccatccacc gtgaccacct gccccgccag cgccagcacg ccagccccgc
    10141 agcccaccac gcaaggccgc cccagcgccc ggctgacaac cgcggcatgg ctggtggcgc
    10201 ccccttgcgc ggtgatgatg gcgcgcgcgc cgatcatgcc gtggatatca tcggggctgg
    10261 tggtctcgcg cgccaggatc actgcctcgc cggcggccgc gcgggcctcg gcctcatccg
    10321 ggctggaaac cacgcggccg ctggcaacgc cggggcaggc gccctcgccg gtggcgatgg
    10381 gggcaaggcc ggccgttgcc ccttcggcaa ggcgcgggcg cagcagttcg gccgcctgct
    10441 cggggctgac acgggccagc gcctctgcgc gtgagatcag cccctcctcg gccatttcca
    10501 ccgcgcagcg caccgctgct gccggcgcgc gcttggccgc gcgggcctgc agcagataca
    10561 gccgcccctt ctgcacggtg aactcgatat cctgcatatc gcgcgaatcc tgctccagcc
    10621 gctcgctggc ggccagcagc agatcgtgcg ccgcgcccac ccgttcgtgc atcgcctcca
    10681 gccgcagcgg cgtgaacttg cccgaaacga catcctcgcc ctgcgcctgc ggcagatatt
    10741 cgccaaaggg cgcgcgcgcg ccggtgagcg gattgcggct gaacagcacg ccggtgccgc
    10801 tgtcggcatc gagattgccg aacaccatcg cctgaatggt gaccgccgtg cccagatcat
    10861 cggggatggc gttgtgctgg cggtatttgc gggcgcgtcg gctgttccag ctgtcgaaca
    10921 cggcgcgcac ggcggccagc aattgcgcgc gcggatcttc cggcaggtgc tgcccggcgg
    10981 ccagcgccac ggcggcgcgc cattgcgcag gcgtgccctc tggcggcaga tgggccggcg
    11041 ccttcagcac gatgccggca tagagttcca gaaagcggcg gtgcgtatcc cgtgcgaagg
    11101 cgggttggcc ggtttctgcg gcaagggcgg cttcggtggc atcggtgatg cccagattca
    11161 gcaccgtgtc catcatcccc ggcatcgaga tcgccgcgcc agagcgcacc gacagcagca
    11221 gcggcgctgg ccccgcgcca aagcggcgcc cgctgccggc ttccagccag gccagcccct
    11281 cggcgatctc ggcctccagc ccctccggca gatcgccgtt ctgataggcc gcgcaggcgc
    11341 gtgtcgtcac cacgaaggcc ggcggcacat tcagccccag cgccagcatc cgcgcgatcg
    11401 accaggcctt gccgcccacc agcgcccgtt cgggcagatc cgcgccatcc aggcgcacag
    11461 tccatgtcat tgccatcatt cgaccctttc gcggcccatg aggcgcagca gatcctcgtg
    11521 cagttcgaac cacacggtgt gatagctgtc gcggtgaata tcgctgaccc attcgatggc
    11581 gccgccctcg gcgcggtcga gcgcggcttc cagcttgcgg ccatagatcg ccatgcgcgg
    11641 cagggcgcgg gcaaggccgg ccagcacggc ttctgcgcgt tcgtgcaggg cagacaggcg
    11701 gtcgatcacc ttgtcgtccc aggcctggtc ggtgtggtcg ttggcgcggc gctcgccatg
    11761 caggtcgatc gtctgccagt cggtgatcac ctgcttcagc tcggggttga tccgctcgaa
    11821 gccgtgatag gccgcggcaa agcctgcatc ggcccgcaag gcggcgcaca ggcgctcgta
    11881 gctggcctgc agcgccacgc gggccagcgg cgtgagggtg aaggcgccgc gcgcttccac
    11941 cgcccgcccc gtggccaccg cccgggccag ttcgcccgcg gcctgctcgg catccagccc
    12001 cgtcagttcg gccaccgccg cgggccctgc atggcgcttg atcgccaggc cgtgcagcgc
    12061 cagatcgcgc gtttccatca tgcctctccc gccagtttcc acacgcgccc ggtgcgttcc
    12121 atgcccgatt ggtagtcttc cgtggtgcgc gggggggcgc tggcttccat gcacacggtg
    12181 tcgccatcgg cgatgccgga gagcttgctg ttcatgaagc aggggatgcc atattcgcgg
    12241 ctgaggatgc ccagatgcga gcgcaccgtg ccgcctgcgc acagcacgcc ggcaaattgt
    12301 tcgaggatgg gggcagtgag ggtgccgccg gaatcgtcga tgatggcgat ggtgccgggc
    12361 ggcacgccct ctgccagcca ggccagcacg cgctcgttgc tgcggatata gcgggcggtg
    12421 ccggtgatgt cggcctcgtg gcgcaccaca ttgtcgccct ggccgcaggg ggtgcgccct
    12481 tgcgcatcgg gcgcgccggt gatcgccccg gggtgctcga acaccaccgc atcggcgccg
    12541 ccggtgccgc ccttgtaggc ctcgggccgc tcggccagcg agatcgccgc cgaccagtcg
    12601 aagcggtggc cgctggccag cagcgcggct tccaccgctg ccacatcggc gtggctgatg
    12661 gcgcaatcct cggcgctgcc gggcgcggca aagacgaaat cgtcgaagcc tgcgccgccc
    12721 tgcgccacat gggcggccag cttgcggcgc accgcctgat gcagcagcgc cagcgttgcc
    12781 tccacgatgg ggctggccgg cggcggcgcg gcgcgcatct cgtctatcgg caatcctgcc
    12841 attgtcctct ctcccggggt tccgctggcc agtgtccgcc atgcgggcgg gctggtcgac
    12901 ggacgatttt ctgtgtgatg attgtctgat aaaattgtgc gacaaggtaa gtcaagcgag
    12961 tcggtaaatc aattgacgac tttggccgac gaatatgtca taccatatcg cagagagaag
    13021 gcgcctgata gatgagtgca tctgaaaaac ctttccaaaa caagcgcgtg ttgatcgtga
    13081 atgacgacgg catcgacggg cccggcatcc gcctgctgga ggaaattgcc cgcggtttca
    13141 ccgatgatgt gtgggtcgtc gcccccgatg aggagcgttc cggcgcgggg caggccatct
    13201 cgatttcgca tcccatccgc tatacgcagc gcgatgaacg ccatttcgcc gtgcgcggca
    13261 cccccaccga ttgcgcgctg ctgggcatca acaccttcct cgaagggcgc aagcccgata
    13321 tcctgttctc gggcatcaac gccgggccca acctggccga ggatgtgcat tattccggca
    13381 cttgcgcggc cgcgcgcgaa ggggcgctgc tgggcattcc ctccttcgcg ctcagccaga
    13441 tgcgcgctta tggcacgccc acccattggg aggcatcggc cacccatctg cccggcgttg
    13501 tggcgcaatt gctggcgctg ccctgggcgc cgggcagctt tgccaacatc aacgtgccca
    13561 acctgccgcc ggcggagatc accggcatcc gcatcacccg gcagggccag cgccggccgg
    13621 gcggctttct gcccgtgcgc cgggtggatg aacgcacggt gccctatttc tgggtgaaga
    13681 tctgccccaa tccgcccgaa ctggtagccg gcaccgatct gcacgcggtg gcccatgggg
    13741 cgatttcgat cacaccgatg cagctggatc tgacgcgcca ccaggcgatt gccgggctgg
    13801 aagcggtgtt cggcgccagc gatggcgcgc cgggcccgct gcttgccgcg gccgatgcct
    13861 gcatataagg cgcgggcgca tgacaagcga tatgcccttt tccatcgccg acagcgatgt
    13921 ggccccggcc agcccgcgcg atacgatcca gcaggtgacc gaggggctga tctattgcat
    13981 ccgggccggg cagatggtgc ccggccagca tctggtcgag gccgatctca cccagcgctt
    14041 cggcgtcagc cggggatcgc tgcgcgaagg gctgaagcag ctggcggccg atggcatcgt
    14101 caccctctcg cggtttcgcg gggcgttcat cgcctcgctc gaccgcaagg gggtgagcga
    14161 cctgctcgac gtgctggagc ccctgtgcat gctggccgcg cgcctggccg cgcaagcctg
    14221 ccagcaggcc gatgcccatg ccaccctgca cgaagtggcc gccctgctgg aaaggcacgg
    14281 cgagaatggc gggcgcgcgg cctatctgga aaaccgccgc cggttctatg acctgctgat
    14341 cgagatgggc ggcaacagcg agctgggccg ggtgatcccg ctggcccgca ctgacctgtt
    14401 ccgcgcccag ttcgacacgg tgcagaccaa ggagcagcac cgccgccatg cccgcggcta
    14461 tgcccgcatt gccgaggccg tggcgcagaa cgatccggcc aaggcggaaa gggcggtgcg
    14521 ccgccatttc gccggcacgc gcgccacgct ggatgtgctg cccgatcagg ccttcgcggt
    14581 ggcgctgcgt tgaggtttgg cgctgaggtt tggcgctgcg atctattcca gatagagggc
    14641 gcgcagctgg cggatatcct gcgcggacag gcccatctcc tgcgccagat agcccgctgg
    14701 cccgcccctg tgccgctcca taaccgccag cgctgcgccg atataggccg ggtctgccgc
    14761 catcagcgcg tggaacaccg gcggcggcag gctggccagc atgcccagcg cgcttttgcc
    14821 gctggccata ttggccggcg cacgggcgct gccacgcatc accgcaaggt ctagatagcg
    14881 gttgctcagc atgtaatcgg cgacaatcgt gtggcgcggc acgcccagcg cggtgaggat
    14941 cagcgcagcg gcgatgccgg tgcggtcctt gccggcggtg cagttgaagg ccagcggcgc
    15001 ctcgccggcc agcaaccggg cgaacatgcg gcgatattgc ccgttcagca gcaccagcag
    15061 gcgcggatag ctggcgatca tcgccgcgcg cacctggccc acgcgccagc cttgcggatc
    15121 gcccggcggc atcagcgcct tcacatcaag cgtataggca tcgaacagct ggcggggggc
    15181 gtgggcaggc ggccagtcgg ccggctcggc ggcgcgttcg cgcgcgtcgc gcaaatcgca
    15241 cacggtgcgg atgccgcggc tttccagcag ggcataatcg gccccggtca gcccgtgcat
    15301 cgcgcccgag cgatagagca ggccccattt cacatgatgc ccgtctgccg tgcgatagcc
    15361 gcccagatcg cggaaattgc gcccgccctg cagcggcagc aggcgctggt gggcggccgg
    15421 cgcgggcggc acaggcagtt gggcaacaag gcccgccggc atggccagca gcagtgcggc
    15481 gcagcagatc atccagccct tgcgcatggc ttgcctccct ttggggccgt tcagccgccc
    15541 agcaccgtca ccgaggacag cgcgtcttcc acgccataaa agccgcccga gttttccgcc
    15601 agcgccacgc gcgcgccggc cacttgccgg gcaccggcgc ggccgcgcaa ttgcgtcacc
    15661 agctcgtgga tctgggccag gccggtggcg cccagcggat ggcccttcga caccagcccg
    15721 cccgagacat tgaccggcag ccgcccgccc agcgtccatt cgcccgccat tgcccgcgcg
    15781 cccgcctcgc ccggcggggc caggcccagc gcctcgatct ggatcgcctc gccggtggag
    15841 gaggcatcat gcacctcggc cagatcaaga tcctgcgggc ccaggcccgc atcggcatag
    15901 gccagcgctg ccaccttggc cgaaacgtgg ccggcataat cggccggatc gcgatcatgc
    15961 cccgaaagcg agcggcaggc gcgcaccctg accggcgcgc cggcgcccag ccgcccggtg
    16021 agatcggccg cgcacagcac ggcggccgcc gcgccatcgc tgatcggcgc gcacatcggc
    16081 acggtcagcg gccaggcgac ggtgggggcg gccagcacct cctcaaggct gagcgcatgg
    16141 cgatattgcg cgagcggatt atgcaccgaa tgggcatggt tcttggcgct gatcgcggcg
    16201 aaatgggcag gggtggagcc ataggtggcc atgtgcagcc gggcctgggc ggcataggcc
    16261 tgcatcagca cattggggcc gggcgcctct tgcccagccg gcacgggcgc cagccgcccc
    16321 tgcgtgcggg ccagataggc ctcggccacg ccgatatcga gcggctgctg gaacacggcg
    16381 aatttcttcg cccggtcctg cgtgtagagc ttttccacgc ccaccgtcag caccacatcg
    16441 gcaaggcctg cgcggatgag atcataggcc agatgcagcg ccatggtgcc gctggcgcag
    16501 gcggcctcca catggaaggc gcgcacgccg gcaatgccca gcgggcgcag cgcatattcg
    16561 ccggggatcg acatttcgcc cgcgacaaag ccctgcacca cgctggaaaa gatcgaggtg
    16621 tcgagcgcgg aaggggcgat gccggcatct gccagcgctt ccttcacggc cgcctgggca
    16681 agggcggaat agctgagatc gaggtggcgg ccaaagggcg tcatccccac cccgatgata
    16741 tcgacggcgc gcatcagatc gtcctctcgc gttggttcca atagggttgg cgcagggccg
    16801 cgcgcagcac cttgccggcg ttggaacgcg gcagcgcctc ggtataggtc agctgcttgg
    16861 gggttttcac cgcgcccagc ctggcgcggg caaaggcgat cagctcctcg ggcgggggcg
    16921 gggcgatgcc ggcgcggcat tccaccacgg cggtcacctg ctcgccccat ttgtcgtcgg
    16981 gtatgccgaa gacggcgcaa tcctgcacgg ccgggtgcgc ctgcagggcc tgttccacct
    17041 cggccggata gacgttgaag ccgcccgaga tgatcatgtc cttcttgcga tcgacgatga
    17101 agaaccagcc ctcggcgtcg cggtagccga tatcgccggt gtggtgccag ccaaagcgcg
    17161 agacttccgc cgtcgcctcg gggttgcgat agtagccggc catcaccagc ggcccgcgca
    17221 caacgatctc gcccctttcg cccggcggca gcaggcggcc ctcctcatcc atgatctcca
    17281 cctgcgagag gaggttggcc tgcccgcacg agccgggatg ggccagctct cctgcggcat
    17341 tcaggtgctg gtgggggggc atggcggtca cgcacatcgg cgcctcggtc tggccgaagc
    17401 tggcgttcat caccgggccg aacagggcga tggcctcctt cagccgctcg gccgacatcg
    17461 gcgcgccggc ataggagaca tattcgaggc tggcgagatc gcgccggcgc acttcggggt
    17521 ggtccagcag catgtagatc gcggtgggcg gcaggaacat gtaggtcacg cggtggcgct
    17581 cgatcgcctc cagcaccgcg cccgcctcga acctgggcag caccaccacc gtgcccgaca
    17641 gcgccatgct ggccagcgcc agcggccctg cggcatgggt catgggggcg gccaccagat
    17701 tgaccggcgg cgccttcatc ggcatcgagg cgaccaggct ggagaccagc gactgccaca
    17761 ccagattggt gagcatcacc ccgcgcgggc gcccggtggt gccgccggtg ttggccagga
    17821 acgccagctc gtcgggcgcg ccgtggcggc ggcggggcac ttcggcatcg tttggccggg
    17881 ccagccagtc gcccagaaag gcatcggctt cggcatgggg ggcatcgatc gtcacatagc
    17941 ggcggatctt cgggcaatcg cggcggaagc gggcgatgcg ctctgccact tcggtgtgga
    18001 gaaacagcgt gtccacatcg agcaggtcga gcagataggc gttctcgtct tccgaggcgc
    18061 gggcattggc catggtccac acgccatcgg cgcgcagcag gcccagaatg gcctcgaagg
    18121 cagccggatc attgcccgac agcaccgcgc catgggcccc tgcggcaaag ccgtcggcga
    18181 tcagcgcatg ggcgatgcgg atggagcggg cgcgcagctg ggcataggtg atgctgcgcc
    18241 cggcgctcac catgcagggc tgcgagggcg cattgcgcgc gccccggtcg aagaagtcga
    18301 tcatgcgcat gggaaggggc ctttcagcgg cggatcgaga cttccagccc ttcggtcttc
    18361 aggcggacga gcggggcggt ggccacgcgc atcagctttt ccacctgggc cagctcgggc
    18421 gaggagagca tcggaaaatc acgcgcgggc gggcccgatt tgcgcatgcg ggcaatgtcg
    18481 gcggcgggca ccagcgcgcg caggatcagc tcgtcctcgt catccgtgcc atgctggcgc
    18541 ttcagttcgg caaggtcggg ctggggcggc gggttgttga cgatatccct ggcgcgcggg
    18601 gcggccatga tgcggtccag cgcttcgggg tcgatggggg cgggcggctc gccataatag
    18661 ccggcggcat attggatcac ctcgtcgggc acggcggaat agcgcttgcc cgtcacgatg
    18721 ttcagcaccg ccagcgtgcc caccagctgg gcaaagggcg tggccatgcc gggatagccc
    18781 agctcgcgcc gcacggtggc cacttcctcc agcaccaccg gcagcttgtc gagcatgttc
    18841 tgctggatca gctggttgcg caaggtgccc atcatgccgc cggggatctg ggtggtcagc
    18901 gaggtgacgt cgtattcgta gtgctgatca tgcagatagc cggccgcctt ggccacgcgg
    18961 cggaaatgat ccgacaccgg gggcagcagc gatgtatcga tatcatgcgt gtggcccagc
    19021 gcttccatgt tgcgcaccat cacgtcgatc gaggggatgg aagggccgtt ggccatcgag
    19081 cgggcggccg tgtgcagcac cgagacaccg cattccaccg catcgcaata ggccttggcc
    19141 gactggccca gcatgttgtt cgaatgcatc tccaccggct tgccgcccgc attggcgaca
    19201 atggcgggca ccagcgtttg gatccgctcg cggtccaaaa cgccgccggt gtcgtagagc
    19261 agcaggctgt cgatctcggg gatggcggcc agcttggcgg ccttgtcggc ccagaaggcg
    19321 tcgtcatgca cgggcgagag ggtgaagagc atcgtgccgg ccaccttgca gtcgtatttc
    19381 ttggagagct tggccaggcg cgccatcttg tcgatgttga acagcacgtc gtaaatccac
    19441 cagctgcgca cgccatggac gcacaggcgc tccatccaca ggtccatcag gcaatcgggc
    19501 gtgaagccga acgttaccga gccattggag cggatgccgc cgcgcaccgg cgtctggcgg
    19561 atctggctca tcatcaggtc gaagccggcc caggggtctt cctggcaatg gcgcaccagc
    19621 acctcaaaca gcgaggagcc ggcaaggctg atggtggaat agccggtcct gtcgatggtg
    19681 ggcagcaccg gataggcctg gcctgcctgc atgcgcatgc cccacaggct ctgctggcca
    19741 tcgcggcagg tttcatcgag aaagcggatg tgcgccatga gggcagcccc ttattgatcg
    19801 agggagggca ggagcacttg ctccagccag cgggtgtgga tggcattggc cctgaaatcg
    19861 ggatgggcga tgatgcggcg gtgcaggcca atggtggtgg gcacgcctgc caccgcaaaa
    19921 tcggccagcg cggcttccag ccggtcgatc gcctcggcgc gggtggcgcc ggagacgatc
    19981 agcttgccga tcatcgaatc gtaatagggc gggatggtgg cgccttcgcc catatgggtg
    20041 tcgaggcgga tatgcgtgcc ggccggcggc gcccagcggc tgatcgtgcc gggcgagggg
    20101 atgaagccgc gctgccagtc ctcggcgttg atgcggcatt cgatggcatg gccggcaacg
    20161 gcaatgtcgg cttgctcgat ggacaatgtc tcgccgccgg cgatgcgcaa ttgccattgc
    20221 accagatcgg cgccagtcac cgcttcgctc accgggtgtt ctacctggat gcgggcgttg
    20281 acctcgatga aatagatctc ctggcggtct acatcataga ggaattccgc cgtggccgcg
    20341 cccttgtaac gcacgctggc ggccagggcc acggcgctgg catgcaattg ccggcgcagc
    20401 gcctcgggca tggcggcaga cggcgcctcc tcgatcagct tctgatagcg gcgctgcacc
    20461 gaacagtcgc gctcgccgaa atgcagcacc ttgccggtgc catcgcccat cagctgcact
    20521 tccacatggc gcgcgcgcgg cacgaagcgt tccatgaaca gcgtgccatc gccaaaggcg
    20581 ctttcggcct cggcgctggc gcgggcaaag ccggcctcca cctcggcgcc gctgtgggcg
    20641 atgaccatgc cgcgcccgcc gccgccggca gaggccttga gcagcaccgg atagccgatc
    20701 tcttcggcca cgcgcacggc atcggcggcg ctggcgatgg aatcgctgcc cggcaccagc
    20761 ggcactttgg cggcgcgggc catggcgcgg gcgcggatct tgtcgcccag cgcctcgatc
    20821 gtttcggcat cggggccgac aaaggcaatg ccatgggccg cgcacagccg tggcagcacc
    20881 gcccgttccg acaggaagcc atagcctgga tggagcccgt cgcagccggt ttcccttgcg
    20941 gcatgaacga tcagctccgg gtcgagatag cttctggccg cagggctggg gcccagcacc
    21001 accacccggg tggcagcgcg ggcggcggcg ctgtcgcggt cggcatcgga aacgccgatc
    21061 accgtctcga tgcccagcgc ctttgcggca tgggcgatgc gcagggcgat ctcgccccgg
    21121 ttggcgatga agagccgttg cagcgccatg gttcaggcca ccggctcaag cgcgaaaagc
    21181 acctgcccct cgctgaccat gtcgccatcg ctggcataga tgtggcgcac gatgcccttg
    21241 ctctcggcgc gcacggcggt gaacagcttc atcacctcga caaggcacag atccgcgccg
    21301 gcctccaccg tgtcgccctt ttcgcagaac accggctcgc cgggcttggg ggcgcggtag
    21361 aacgtgccga gatagggcgc gcgcaccagc gtcaggccat cggtctcggc cggtgctggc
    21421 gctggcgctg gcgcggcggg ggcgggttgc gtgggggtgg cgggggcagg ggccgccgga
    21481 gcaggggccg cggccggctg gggcgtggcg ggggcgccat cccacggcgc gctggcctgg
    21541 ggatcgcggg agaggaacag ttccagccct gccgccttga tgtgcagctc ttccagatcg
    21601 gaagcctgga actggcggat cagggcttcc agatcctcat tgtctcgtgg ctgggccata
    21661 tcgcttgtcc ttgtgtgatg ggcgggcggc gggcggttca gccgcggcgc agggcctcga
    21721 tgtcggcggc ggcaaggccg atgcccctgt ccttcagcag ccgcgagcgg cgctgctcga
    21781 ggcggtagag ggcgtcggcg cgcgggtctg cggggtgcca tttcagccag gtgtcgctca
    21841 gcccgcgata gagcgggtcc atctgcgcgg gccggtgggc gcgggcgcgg gcgttgtatt
    21901 cggccagcgg cagggggccc tgcgtggtgg tatagctgtc gcgcttggcg gcgtggcggc
    21961 tggtgccggg caggatgccg ggcttttgcg cggcgccctc gccatccagc acgctgaagg
    22021 ggatgtggct gagatagcgg cgcggcaggt tgttgtgctg catcatcatg tgatcggcca
    22081 gatgctggct gggcagatcg acatggccaa ccagctcgcc caggaaatcg cggccgaatt
    22141 catcgttgag cagcgggcgg aagcgatcgg cgcgcgggtc gatcgtcatc cagtcgtcct
    22201 gttcatacac gcccgccaca tgggcgaaat ccttgatgat cgagaaatag acgagggcgc
    22261 cggcatccat catctcgtcg cggctccagc cggccacgcg cttccacagg gccgcgatgg
    22321 ccgcgcgcac ctgctcggtc agcgtctcga atgtggcggc cagttcctct gccgagccca
    22381 tgcccaccgg catatagcca tccgagagca catccgacgt gtaaagcccg acgccgacca
    22441 gcttgtcggc ggtgaattcc gggcgggatt caaagctgcc ccagtcgtcc atcaggtaga
    22501 aatggcaatc ggccacttcc atcgtcacgg tcaggttgtt gtaggcgatg ggatcgccca
    22561 cgccatcgag ccagggataa tcgccggcgg ccagatcggt gaaatcgcgg atgatcagct
    22621 cgcggttgtc ggccaggcga taggggccgg aattgttgag cgtgacccgg ctttcgcacg
    22681 agatcatgaa gccatattgc gacactgccg ccagaaagcc ctgcgcggcc ttgtgcagcc
    22741 ggtcgcccgg cgcgcagcgg tgggcatcgg cgtggaacac ctgtacccgc cgttccggca
    22801 gcaattgcac gcgctggccg aattcgcgcg cgttcagatg gccatcctcg cgctgctggg
    22861 ccagtttgaa gcggcgccag aaatccagca catagcaggt gtcctcggcc gcatcttccg
    22921 ggcgcagcat gccgaaattg atcagcatct cgcggcccag cagatagaag gtgaccatgc
    22981 cccagccggg cagattgccg aatttggcgc ccatttcgcg cgcgcgatcg ccgatctcct
    23041 cggccggcat cgccgcctcg atcttgcgca gcagcgccgg gtagcgatag aaacacatga
    23101 ggtatgacag cagcatgtag gccggcaccg ggaacagctt cgattcctgc acggtgcgcg
    23161 tcacgcacag ccaataggtg tcatccccga tggtctggat ggcgttgttc gcttccagga
    23221 tccgggcgta ggtcaggccg gattggccgg ccccttgctc tgccagactg ctcatcccac
    23281 tcatgctctt tcttgggccc gatggagcga gccgattttt gatggatcaa attgtcggac
    23341 gacattgccc gaagaagctt ttaggcaaag gcgaaaagct ttgcaaccgc ccttgtgcct
    23401 gtcggggcct ccatgcaggt gtttgcctcg gttttgcctg gcgctgaaat ggcgcaaatc
    23461 ggccgggtct gccattaacg gaaatccgtt ggcgggggcg gcggggtggc ttttggggcc
    23521 ccgccatgcc gccggaaccg gtgcgaatgg cccgtttgcg cggccggcgc gggggtgggt
    23581 gtggcggcct cggcagcgaa tcggatcgcg ccataatcgt cgatgaaatt gtttgacgat
    23641 atggcgtttg cctctatctt ccccgccaca atgaccggcc cgcccaagcg gccggcaagt
    23701 ggagagtgtc atgcgtttga accgggctat tgccgaggcc tcgcaccgcc tcgtgatcga
    23761 ttgcgcgctg gataccgcct cgatcttctt ctgcgatcat tcgaccgggg atggcagcct
    23821 gtcctatctc catcacgtca acatcaccag cgaggcgcag cacgcctatc gccaccacgg
    23881 cctgttccgc cacgatccct tcgtggcgct gacgcgtggc gatgccagcg ggcgcgggcc
    23941 gggctttgtc gcctgggggg atcggcggct ggaagcggca acgcacagcg cgcgcgatta
    24001 tcacagcttt cttggccagc atggggtggc ggtggcgggc gcgctggggc ggcgggtcag
    24061 ctcgcaggtc agcctgatca tcggcgcgca ccggcgcccg cgcggccagc attgcggcga
    24121 ggtgccgatg ggcctgctgg aacagcgcat caacgccctg tgcgagatgg tgatcgatgg
    24181 cctgttcgag gaactgctgg gcgatgccgc cggccgccag gtgttggccg aggcaggcag
    24241 cccgccgctg ctggccgagc gcgagagcca gatcaccgcg atgatctgcc agggcctgcg
    24301 caacaaggag atcgcctggc ggctgggcat ttcggaatat acggtggaga accacttgcg
    24361 gcggatctat cgcaagctgg gcattcacaa ccgggcggcg ctggtggcgc gctattccgg
    24421 ccgctgccat taggcggcgg ccggcggcgg ctcagaaatt ggccgagcgg atctgccggg
    24481 cggccacgct ggcgcgcagg aaagcccaga tcgcccgttc cagatcgcgc gcggcgggcc
    24541 cttctgcctg ttcgatgcgg gcgtgcaggg cgatcagctg gccgcgcagg cgatcattgg
    24601 cggcggtaag cgcgctgatg cgcaggtctt cctccgccgc ctctggcgcc agagcatcgc
    24661 cgccgcgttg cagcagggcg cgcaaggcgc ggttttccgc caccagccgg gccgccgcgc
    24721 catcccaggc atcggcgatc atatccagca tggccgcgcc catgccggcg gtgttggcgc
    24781 gaaagccggt cagttcgggc tgcagttcct cgcgcaggcg gcgggccagc tcggcggcgg
    24841 ccagcgagac atcgggtttc atggcagcgt ctccgtcaac tggcccatca gttcaagggc
    24901 agccttgtcc tgcgtgttgc gcagccacca ggcggcatag gcgtggatca cctcgcggcc
    24961 ggcgccgctg gcccacacat gggcggccgc gttccagatc gcctgcgcct tcacgcaggt
    25021 gaacaattcc caccaccaca gcgcctgtgg gtcggcctcc agcccgctgg cagcttccca
    25081 caggcgcacc gcctcggcat ggggggcaag gccggatcgg cgcgcgtcct tgccgaagca
    25141 gaagctgcgg gtgaggctcc aggccagatc ctccagcgga tcgccgcaat gggccatctc
    25201 ccagtcgagc acggcctgca ggttgccggc atcgtcgtag aggaaattgc cggcgcgaaa
    25261 atcgccatgc accagcgcca gcttggcagc caccggctgc cagcgttcca gccggcggat
    25321 ggccgcgcgg atgatcggct cggggccgat atcgtgccgg tcgagcgagg ccacccaata
    25381 ggccagttcg gctgccgccg tgccttcacg cgtggccggc cgggtgaagg agaggtcgag
    25441 cctttccacc ggcaccgcgg ccaggcggcc catcgtgccc cacatctggc gcgccacatg
    25501 cggcagcagc ggggcccagg cgggctgctg gaagagatat tcgctgttgt gaaacccgcg
    25561 caggtcttct gccagcgaga tcgcgccgcc cagtggcgcc gggtcctcct ccagcagcag
    25621 catgcgcggc accggtatgc cgtggccata aatggcgcga taggcggcaa attcatgcgc
    25681 gcggtcgctg tcgacattgg aggcgggcgg atcgcgccgc aagatcagct gcaggcgctg
    25741 gcgggtgccg gcggcatcgg tgtggatgag gtcgaagcga taggtctcgc gcgaggcgcc
    25801 catcggcagg cggctgaggc cctctatcgc cagatcgtgc caggtgggca tgcgcgcggc
    25861 gagataggcg gtcagctggt cggtggtgat atcggccatg gcggtgctcg caagggggaa
    25921 gggggcgggc tgtttggcgg cccgctgccc ctttccggaa aaatcgcgtc agaagcggta
    25981 gttcgcgctg aggccgaagg tgcgtggcgg ggcctgcacc agatagtcga aaccgaagga
    26041 ggcgcgcagg tccagcccat cggcaaaggt cttggcgttg aacaggttgt tggtccacag
    26101 ccgcagggtg tagcgctgcg tgtcatagct cagctgggcg ttcaccttgg cgttgggcgc
    26161 ctgggtgagg ttctggttgc cctgcagggc gttgtagggg gtgaaataga cccggtctgt
    26221 gtagaccacc gaggggatga agtggatttg ccccttgcca agttccatca ccagccagtc
    26281 taggtgcccg ctgaactgcc attcgggcgc atagggcagg cggttgcccg ccacagagcc
    26341 cagcagcagc tgcgccgatt tgtagcgcgc atcgatatag gaaccgttca gcgccagctt
    26401 gaggccgggc agcggtgtcc agctgccatc gatctcggcg cccttgatct gcgatttggg
    26461 cacattgtcg agcagcacct gcgcgccttc ggtctcgttg atctgctggt tgcggtaatt
    26521 catcaggaag gcggccaccg ccagcgacag gtggccatcg gcaaagcggc ccttggcgcc
    26581 gatctcgtag gcatccacgc tttcgggcct ggtggtggtg atggtggggc caaggaagca
    26641 ctggccgcaa taggccgcgc tgcgatagcc gcggccatag ctggcataga gcatgtggcc
    26701 gccggtgaag gtgtagttga tggccagccg gccggtgagc gcctggctgc tgagcgggat
    26761 ggtgggcccc gatgtgtagc cgccggtggc cgcattatac gcgccgaaga cataggtgcc
    26821 ctggctgaag gggaagatcg ttgtgtcggg cgtgccggtg tagaggctgg agggggtgag
    26881 ataggaggtg tagttctgat agcgcagccg gtcccaggtg tagcgcaggc ccaccgtcac
    26941 gccgaggtga tcgccgaatt tctggtcggc ctgggcgaac agcgcatagg atttgcggat
    27001 ctggtcgtag atctggtggt ccatcgcggc gccatccagc agccagtaat agctgttcga
    27061 ggcatccttg tcgtagccat aatagaggcc ggcctgggca ttggtgtggc cggccgaata
    27121 gctggcgcgc agctcctggt tgaacatggt gaagcggttg ccataaagcg tgtcgagcgg
    27181 ctgctggtag agcggcgatt ccagccctgt gccttcctgc gtgaaggcct ggcgcacatg
    27241 gtcgtaggag gtgagcgaca tcagcgccac cgcatcggag agctggtatt tcaccgtcag
    27301 ctcggcgcca taggcataga ccagcgaatg gcccaggcgc tggctgtcgg tctcgaagaa
    27361 gcccagcccc ttttgcgccc ggctggtgcc cagcaccgga ttatagccat cagccccggt
    27421 gcccagattg tagacgctgg cctgcgtggg gttggaacgg ccgatcgtgc ccttcagcgt
    27481 gatatccagc cggtctgtcg gcttcactcg caagatggcg cggccggcca cggaatcggt
    27541 ggaattggca ttgggctggc ccagcgcgat attgtggata tagccatcgc catgggcata
    27601 gttgaaggcc acccgcaggc cggccacgcc gtccttcagc gtggtttcca ccgcgccttc
    27661 ggccgtggcg gtgttatagc tgccatagcc cagctggata ttgccattat cgcccgacag
    27721 gcccggcttg cgcgagatga agttgatcgc cccgccggtg gtgttgcggc catagagcgt
    27781 gccctggggc ccgcgcagca cttccacccg ctccagatcg aacaggttga ggccgtgggc
    27841 ggcgcgcgcg gcgatatagg catcgtcgaa atagatgccc accggcgagg cctggttggg
    27901 gttgtgctcg ttggccacgc caatgccgcg cagggtgaaa ttgggctggc tttcgccata
    27961 ggggctgttc acctgcagcg agggcacttc gccggccagc gcgctggtgt tggtgatgcc
    28021 gcgcttcacc agatcctcct gcgagagggc ggacatggcg atgggcacct tcatcagcga
    28081 ttccgaccgg cgctgggcgg tgacgatgat ctcgccattg ccggcagggg cgctggtggt
    28141 gggggcatcg gcgttttcgg ccagcgccgg cgccccccat gccagcgatg cggtggcaca
    28201 cagcagcagg cttttcatcg acacagacat ccacaccctc ccgttatgtt gtttcaggcc
    28261 cgttatgttg tttcaggcgt ggttatgggc gtgttcggcg gcaatggcga agtgacggat
    28321 tatcgttatc ggcaagggcc gcctgcgcca ggcgcgccaa ggcaccggca gg
//
LOCUS       DASRYE010000226        32077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_184225,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000226 DASRYE010000000
VERSION     DASRYE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 32077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32077
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184225"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VFF98_11570"
     CDS             <1..139
                     /locus_tag="VFF98_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZU64315.1"
                     /translation="HLAGMKDSKTIIAINKDEDAPIFQVADIGLVADLFSAVPEIAAK
                     A"
     gene            512..2890
                     /locus_tag="VFF98_11575"
     CDS             512..2890
                     /locus_tag="VFF98_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64316.1"
                     /translation="MTGAKKAAANTADQPIDHLILGGGAAAVSAAIALRLEDPSATIT
                     LLCADEGLPFYRPALSKQALLGTADEAQILIHPASFYAEQNIRLELGTTAIALDTEAR
                     VVSCASGARFSYRRLLIATGAKACRLALPGADLPGIHHLRDRAECAAMRREIAAGARR
                     AIVLGGSFLGMEIAMSLRDLGLEVTVIEQREQVLPHVESGRVSDFFRRHAEERGLKVL
                     LCDTITALHGVGRLSEVETRSGQRLACDLLVVSIGVEPAGAFLEGSGIACAQGLVLVD
                     EQLRTNRPDVFAAGDITSFYDPVFGRRRHIEHWDNAIKQGRLAGRNMLGQRLRYDEVS
                     YFFCDVGDISFSMLGAPEEADERIARGTLASRSLALFYLRNDVPRALLSVGRPAEETR
                     SIEGLVRYRVNLRACKARLADPAFPLEQIPTQTVLVLQGGGAMGAFECGVVEALEEAG
                     IFPDIVAGISIGALNGAIVAGNPRDASAALVSFWSQLAIPSPAALIGEEAARALTAAQ
                     ILAFGVPHFFRPRWMQPFAASLPSSWTSFYDIGPMKELIARHVDFARLKASPVRLLVG
                     AVNVTSGELEVFDSYTDDLTPDHVLASGSLPPGFPWTEIGGQAYWDGGVVSNSPLDIV
                     MDRCGPDGKQVYIVDLFAGRRPLPTNMMEVLARRDEIVYSERVRSDLRLREMQGAYRE
                     LVGRMLELVDPATRARIRQRPLYIELMGDGAATGITRFIRQGRDGEPSSRDYDFSDIA
                     IRANRQQGLALARATLAERAEAPAAPRAAADAPAAPAPVPLRRADAGRNRPE"
     gene            complement(2853..3629)
                     /gene="fabI"
                     /locus_tag="VFF98_11580"
     CDS             complement(2853..3629)
                     /gene="fabI"
                     /locus_tag="VFF98_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZU64317.1"
                     /translation="MTEPLVNLAGKRGLVIGIANDHSIAAGCAEAFRQCGARLGASYL
                     NEKARPFVEPVAQRLGIEWLLPCDVRVPGQLEALFAEAGTRWGGLDFVLHSIAFAPRE
                     DLHGRVVDSSQQGFAEAMDISCHSFIRMARLAEPLMADGGCLLCLTFYGSERVVEHYN
                     LMGPVKAALESTTRYVAAELGPKRIRAHAISPGPIATRAASGIDRFDEMLDAARARAP
                     EQELVEVADVGALAAFLVSPAAKRITGTIIPVDSGQHLLA"
     gene            complement(3643..5394)
                     /locus_tag="VFF98_11585"
     CDS             complement(3643..5394)
                     /locus_tag="VFF98_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU64318.1"
                     /translation="MPQSAATPAADPLDGLAETFDRAAVAAIAQLTHGLSPGTLAQAC
                     SDWMLHLACSPGKQMQLGARAAQDWARIAEQLLGSACGARAPQAIQPLPQDHRFDNPA
                     WQQPPYDLLVQSFLMAQQWWHAATTGVRGVERHHEDMVAFAARQLLDVLAPTNFIATN
                     PVLQQRIAETGGRCLVDGLHYLLEDMGRLAHGEPPAGTDGFAVGEEVAATPGQVVYRN
                     ELIELIQYTPTTGTVRPEPVLIVPAWIMKYYILDLSPDNSLIRWLVGQGYTVFAISWH
                     NPTGADRNLDMEAYRRLGPLAALEAIGEITGAGAIHALGYCLGGTLLAITAAALAREG
                     DRRLASVTLLAAQTEFSEPGELGLFIDESQLNLLENMMWSRGYLDSSQMGGAFQILRS
                     NDLVWSRVLTTYLMGEREGMNDLMAWNADGTRMPYAMHSEYLRRLFLENELAHDKYRV
                     DGHSISLADVTAPIFAVGTERDHVAPWRSVYKIHQLSHGPITFVLTSGGHNAGIVSEP
                     GHPHRSYRLLTRAEGQMGYDPEEWLLHAEAHQGSWWEAWGQWLGAHSGAPVAPPAMGS
                     AQFPPLAPAPGSYVHER"
     gene            5618..6331
                     /locus_tag="VFF98_11590"
     CDS             5618..6331
                     /locus_tag="VFF98_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZU64319.1"
                     /translation="MRSKLYASPQEALAGLLHDGMVVMSGGFGLSGNPESLIPQIRAA
                     GVKGLTVISNNAGADGFGLWMLLESRQIARMISSYVGENKLFEQQYLSGELELELTPQ
                     GTLAERIRAGGAGIPAFYTKTGVGTVVAEGKPTEIFEGEEYVRETWLRADLAIIKAWR
                     ADPAGNLMFRKTARNFNPNMATAAKITVVEVEEIVPEGTFDPDCIHTPGIYVDRIVLS
                     TINEKRIEKLTTRVKEPAQ"
     gene            6328..6978
                     /locus_tag="VFF98_11595"
     CDS             6328..6978
                     /locus_tag="VFF98_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZU64320.1"
                     /translation="MSWNRDEMAARAAQELQDGFYVNLGIGIPTLVANHVPVGITVTL
                     QSENGMLGIGPFPYEGEADPDLINAGKQTVTALPTSSFFSSADSFAMIRGGHIDMAVL
                     GAMEVAANGDLANWTIPGKMVKGMGGAMDLVAGVRRIVVVMDHCAKDGSPKILPACTL
                     PLTGRGVVDLIITDLAVIAVDRKAGGLTLVETAPGVSVDEVVAKTGAPLAITQGAA"
     gene            6978..8180
                     /gene="pcaF"
                     /locus_tag="VFF98_11600"
     CDS             6978..8180
                     /gene="pcaF"
                     /locus_tag="VFF98_11600"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZU64321.1"
                     /translation="MAEAFICDAIRTPIGRLNGSLATIRADDLAALPLRALMARNPGA
                     DWAALDDILLGCANQSGEDNRNLARMAGLLSGLPKDVPGATINRLCGSGLNALGLAAQ
                     AIRSGDADFLIAGGAESMTRAPFVMGKAASAFARDQKLEDTTMGWRFVNPAMKAAWGV
                     DTMPQTAENVAAEWGISRAAQDAFALASQEKAAAAQASGRLAAEILPVDVPQKKGAPL
                     VFAADEHPRATSLEALARLKPVVHADGTITAGNASGLNDGAAALVIASEAAARAHGLT
                     PRARVVAMASAGVAPRVMGIGPVEATRRLLARTGLSLAAIGVIELNEAFAAQALACLQ
                     DLGIDPADPRVNPNGGAIALGHPLGMSGARIALSAVEELQRSSARHALATMCIGVGQG
                     IAVLLERV"
     gene            8226..8993
                     /locus_tag="VFF98_11605"
     CDS             8226..8993
                     /locus_tag="VFF98_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU64322.1"
                     /translation="MSTSDPDFMQSLARGLQVLEAFAELGPGQTIASLARHTGLARGV
                     VARCLHTLAATGYVEQAGRQFGVRGKVLRLAEGFLADGSLPALAQPLLEQLRDELGES
                     CSLGVLDGAQVLYVARASRSRIMAVGLHVGSRLPAWCTSMGRVLLAALPAAQRAALIP
                     ATLPAHTAHTITDPARLAAVLDEVAHSGMALVDQELEVGLRSVAVPVRDGRGQVVAAL
                     NMGVNALAHEPEALRQTIAPRLRQAAERLGASLPSRD"
     gene            complement(9073..10566)
                     /locus_tag="VFF98_11610"
     CDS             complement(9073..10566)
                     /locus_tag="VFF98_11610"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZU64323.1"
                     /translation="MRLIDHVIVGGGGVPARKSPVFDPNTGAVQAEVALGDAALLDAA
                     VAKALAVQPAWAATNPQRRARVMFEFKRLVEANMQELAELLASEHGKVVADAKGDIQR
                     GLEVVEFACGIPHALKGEYTHGAGPGIDVYSMRQPIGIGAGITPFNFPAMIPMWMFGV
                     AIACGNAFILKPSERDPSVPVRLTQLMLEAGAPEGVLQCVHGDKEMVDAILDHPAIGA
                     ISFVGSSDIANYVYERGAANGKRVQAMGGAKNHGIVMPDADLDKVVNDLVGAAFGSAG
                     ERCMALPVVVPVGEGTAERLIDRLLPEIESLQVGVSNDPAAHYGPVVTAQHKARIEDW
                     IAKCEAEGGKILVDGRGFKLQGHENGFFVGPTLIDHVTPEMDSYHNEIFGPVLQIVRA
                     ADFETAVALPSKHQYGNGVAIFTRNGHAAREFAARVNVGMVGINVPIPVPVAYHTFGG
                     WKRSAFGDTNQHGMEGVKFWTKVKTVTARWPDGSADGGNAFVIPTMG"
     gene            10665..11555
                     /locus_tag="VFF98_11615"
     CDS             10665..11555
                     /locus_tag="VFF98_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU64324.1"
                     /translation="MQDADWSDYQAFLAIARAGQLAGAGRMMGLNATTIGRRLRRLES
                     RLGTTLFEQTREGQLLTPAGEALLAAVEQMAQAASGVEPASSANGPSGSVRISVSEGF
                     GSWIVARHLGDFAKRYPGLAIDLVASNGFLSPSRREADIAVLLSRPQTGPLIARKLAD
                     YALRLYASPAYLAGHAAITGPACLAHGHQLIGYIPDLVYAPELNYLTEIAPDLAASLR
                     SSSILAQHRLLAAGAGIGVLPCFIGDADPALVRVLPDRTITRAFWLVTHKDTHQLQRI
                     RLVTRWLADIVKADARLLAP"
     gene            11721..12128
                     /locus_tag="VFF98_11620"
     CDS             11721..12128
                     /locus_tag="VFF98_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64325.1"
                     /translation="MAAFTFHHGGVSVPSLDEAIAWYGHVLGFELERRFFIEAAGSHT
                     AMVRKGPLRFEIFEVAGAAPLPEDRRHPPRDIKTHGNKHVAFRVADIEAFLVEMEEKG
                     ADIAFVVREAFGKGCFIRDCAGNLIEFVEEPEG"
     gene            complement(12178..13308)
                     /locus_tag="VFF98_11625"
     CDS             complement(12178..13308)
                     /locus_tag="VFF98_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64326.1"
                     /translation="MAKAQKICIIGGGVGGLALAIGLHQRGLAVEVVERDKDWKVYHV
                     GIIVQANFIRALAELGLGDAAVAAGYPYKGARFKDAAGNTIAELPGDAQVDGLPCDLG
                     LTRPALHEVLTQKVRELGIAVRLGVTFTAFEDKGDGVDVAFSDGTTGHYDLLIGADGN
                     YSAVRKALWPDARPQFTGQGVWRYNVPRPADLEWSDIYIGNGQNGFNGKAGYCPLTPS
                     EMYIFAVIEEKGNPRFAPETLAEEMRKRLAGYGGLLGEAAKAVIDPALVVYRPLEACI
                     MPAPWYQGRVVLIGDAAHSATPHLGQGAAMAVEDAVVLAEELATRDLDEALAAFMQRR
                     FERAKLVGTSSIQLGEWEMHPETAGDPVELTARIRAKLAEPV"
     gene            complement(13320..14381)
                     /locus_tag="VFF98_11630"
     CDS             complement(13320..14381)
                     /locus_tag="VFF98_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64327.1"
                     /translation="MAITGIRRLVFLVDDLTTTTRFFTDYGLSFIAGDATSSRFEVMN
                     GAEVLLLLRGHPDLPQGHAQTGIGVVECVWSVDNAAALKRLTDDLARDHQLATDAEGV
                     VHFVTPFGQAVALQHWTPRAVHGAPSPANTPGLVGRLNQPRKWLARATPKRIHHAVWT
                     FPDVQEALEYYRDRLGFRITDVQKGFGVYMRASAAFEHHNIFLANAQAPMPGFDGTLK
                     FHHANFELEDIDEIMVGKNHLDRLGYDFAGNWGLGRHRLTSAAFLYLNMPALGGDMEY
                     GADCDCVDDSWKPRVWDGAFGTAIFMHDLPQWMMPTPNWDVTYARDEQLRFLPADPKP
                     ANTTPAGVPATDIAAEAAE"
     gene            complement(14517..14747)
                     /locus_tag="VFF98_11635"
     CDS             complement(14517..14747)
                     /locus_tag="VFF98_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64328.1"
                     /translation="MSQNSKPPQMRVLDANDKELMTVRKIEREGDALVIRGKIFGAMP
                     MVAKLTPAEARAALKLLDAKTILFLVTLLFRR"
     gene            complement(14747..16330)
                     /locus_tag="VFF98_11640"
     CDS             complement(14747..16330)
                     /locus_tag="VFF98_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZU64329.1"
                     /translation="MSNYDIVVMGAGHNGLTAAAYMAKAGKKVLVLERKPHFGGGVST
                     RELIQPGFWHDEHSNVHIMIQGNPMLREDELGLLSRFGLEYIYPDLPHASIWEDGTIV
                     RSWKDLDRTCEEIASFSPKDAEAYRGFARASMAALPMFMSGLYMPPFPMGAFVAMMDQ
                     SDEGRFLLDVMSRSALDVVEQYFESDLLKLHIVRMVTENLQMPDELGTGMGAFLMPGI
                     IHTYGCSMPKGGSGQLSKALVRAIEHWGGEVRCNAEVARVIVSGGKAAGLELTTGETF
                     MAKDGVIGAIHPHVLRKFVAETPEPVLQRAERVTPSTFSINLTHLTLKERLRLKVGNE
                     TNAMMTELMDFYTIREMLLEYDKLRRGLTAPRLIAGGDNTIFDPSRAPEGAGVFYGVN
                     FAPYELFEGGHAAWDEKKEEVADAALAQYRKFYANLTDDNITGRLIRSPIDHERDSPA
                     SFLKGDIHGCAPFMYQSVGHRPTPDLGSFRVPQVEGLYLVGPFMHPGGGVFGAGRATA
                     IQMMDDMGIDFDKVCGGAI"
     gene            complement(16327..17139)
                     /locus_tag="VFF98_11645"
     CDS             complement(16327..17139)
                     /locus_tag="VFF98_11645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64330.1"
                     /translation="MAQDAIRMDGKVALITGGAGGIGAATARLIAARGGKVAIADIAF
                     ERAEALAAELPDALAIRLDLEREESILAMVAATLSHFGQLDVLHNNAALLSPEIARAD
                     GDVEAMDTALWDRTYAVNVRGTMIGCRAALPHLRATHGNIVNTVSNLALQGHIIQAAY
                     SSSKAAIIQMTRAIAASHGIHGVRCNAVAPGMTMTPALREAFPPPLRAAVEGETLRTQ
                     LGEPEDIAEAVAFLASDAARNITGQVLVADGGCASHVPGLAPFRAFFSGENH"
     gene            17245..17679
                     /locus_tag="VFF98_11650"
     CDS             17245..17679
                     /locus_tag="VFF98_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64331.1"
                     /translation="MTDGIPQHLGARIAALAEEMARHGNHREAPVREVMAKLGDRWST
                     LILIVLEMGEWRHADLKRVLVQLSAEKAISQRVLTLKLRALERDGFVERRATADVPPR
                     VSYRLTALGERLAAEARRMIGWVNSQAGAIEAARQAFDMRED"
     gene            complement(17706..18716)
                     /locus_tag="VFF98_11655"
     CDS             complement(17706..18716)
                     /locus_tag="VFF98_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64332.1"
                     /translation="MRFQRLDLNLLVALDALLSEKSVSLAADRLCLSQSATSSALGRL
                     RDYFGDELLVVKGRNMVLTARAEELIEPVRTVLEQIRTTIAIAPAFDPATADRLVRIM
                     ASDYSTQVLLAGALVMLEKEAPHLRFEIQPMSDNVVEALERGQIDLLLTIDYAISADH
                     PSQLLFEDDYVVVGDAANPAMAGDMTREKYFALGHVTARFGRARTPAFDDWFVRRQKT
                     QRRIEVVAPSFLSLPGLVLGTQRVATMHRRLATQVTKNLPLVMREMPFDIPPIREAIQ
                     WHLSNNNDRALRWVVERLSAYAQEGGPAARGNGNVVPIAPLAEVHRDAIAAQFQQLNS
                     GR"
     gene            complement(18732..19685)
                     /locus_tag="VFF98_11660"
     CDS             complement(18732..19685)
                     /locus_tag="VFF98_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZU64333.1"
                     /translation="MGIIVTGAAGFVGRRIVAGLLAQGHDVVAMDRDVAALETGAGLL
                     AIAGDLADADVRAAALAAPALAGGVAGVIHLATVPGGAAEADPAASRRINLDAMYDLM
                     EDVAARKGPGGTVPRFVYASSIAVFGDPLPAFVDDASPLSPKMIYGGHKAMMEDAVAL
                     FANRGWLEGVTVRLPGILARPKGPSGMKSAFMSDLFHALAAGEAFTCPVSAGATIWAQ
                     SVARCAANFLHALTLDTALLPPTRAVTLPALRVTMGDMAAEIARQCGVGTDLVSYRPD
                     AALEVAFGAQPPLSTPAAEKAGFAHDGDLATLVASALQTLE"
     gene            19875..20996
                     /locus_tag="VFF98_11665"
     CDS             19875..20996
                     /locus_tag="VFF98_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64334.1"
                     /translation="MEGLRTLIVGGGIGGMAAAIALAERGAAVELIDLDPNWRVYGAG
                     ITITGPTLRAYRRLGLLDQIKAQGAITTKTRLFRYDGTHIKDLDEPVIEEGLPATGGI
                     MRPVLHRIMQARVQELAIPVRLGLTVTALENTGAGVDVTFSDGSQGHYALVVGADSVF
                     STVREIAFPHMGAAQPTGQGCWRISIAKPPGLEYGEFYLGHDNPCGITACAPDSVYMW
                     MLTRHVERETFLTEAELYDELKRQLADFGGNAGWIRDHMTMDDWINYRPLAAVLQPAP
                     WSVGRIVLLGDAVHATTPHLASGAGMAVESGIVLAEELERADSVEAALAAYEARRFDR
                     CRDVIESSVKIGQLQLDHASPQAHAEVLEGALARLNQPF"
     gene            21300..22898
                     /locus_tag="VFF98_11670"
     CDS             21300..22898
                     /locus_tag="VFF98_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZU64335.1"
                     /translation="MSYFSDLGLAEPILRALAEKGYADPTPIQRQAIPALIEGRDILG
                     LAQTGTGKTAAFALPSLHRLAADRKPKKPASCRMLVLSPTRELAAQIAENMRFYAKHL
                     PLSVQCVFGGVPAPKQARALVPGCDILVATPGRLLDLIDSRALTLRDVEIFVLDEADQ
                     MMDLGFIVPLKRVARLLPQQRQSLFFSATMPQAIEELGKQFINNPVKVEVAPQSTTAE
                     RVEQNAIFVSQPEKQALMTIILRNGLASNEIDRCLVFCRTKHGADRIVRFLTPAGIPA
                     AAIHGDKSQAQRTAALKGFREGNVRVLVATDIAARGIDVTGVSHVFNFDLPNVAEQYV
                     HRIGRTARAGADGIALSLVAEDERPYLKAIERLTGVKLALQPLPEDFLKEASRLPAPA
                     KRARGGEGNGGRGEGARGGRDGGRDGGRGQGGGGRSEGGRSEGGQRDGGQRDGGRGAR
                     PEEARAKRRFGEGREERVRDGQRSSGQRENDRARAERRQTERRAGEQRADQRSARPSA
                     RSNFDPLRENTDAPARRQGQGRRG"
     gene            22969..24480
                     /locus_tag="VFF98_11675"
     CDS             22969..24480
                     /locus_tag="VFF98_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64336.1"
                     /translation="MSASIIIGEDSKGSPLRIDIEELLATRLLVQGNSGSGKSHLLRR
                     LLEESAALVQQVVVDPEGDFVTLAEPFGHIVVDGAAYTTAELVRLANRVRQHRASVVL
                     ALDGLEIEAQMRCAATFLNTLFDAPREQWYPALVVVDEAQMFAPAAAGDVSDEVRRLS
                     LSAMTNLMCRGRKRGLAGIIATQRLAKLAKNVAAEASNFLMGRTFLDIDMARAADLLG
                     MDRRQAETIRDLARGQFLALGPAITRRPVSVRIGGVRTKSQAISTGLLPPPEVTGEDL
                     HALLHAAPSEEEAALAEAPARLLPRADAGTLIDQISSFAMAAAPGEEPLPASPGDDLF
                     AAASVPAPPSLPPEEVQAILAEIMADIATEPDCTFQPAASLFQDFSVRCRMRRIGNHV
                     GDAAQFRRRFALAVAGIYAPQEERWAALLGLAGGVPEDLLAPFLVIARAAMEEAPCPD
                     DEALARAYGTRSPGRIRRLLEHLEKSGLIVVKTDYGGRRAIGIPELGVSTAAV"
     gene            complement(24502..26136)
                     /locus_tag="VFF98_11680"
     CDS             complement(24502..26136)
                     /locus_tag="VFF98_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64337.1"
                     /translation="MIRLADLALPLDHAPEALEAAAAARLGITPDRLLRLHVVRRGND
                     ARKKAAIRLVYVLDVEVAEEAEVLARLADDPHVGPAPDTAYSLPVMAPAGYAGERPVV
                     IGAGPCGLMAGLILAQMGLKPIIIDRGKAVRERTKDTWGLWRRRELTPESNVQFGEGG
                     AGTFSDGKLYSRIKDPRHLSRKVLTEFVKAGAPDDILYEAHPHIGTFRLVTMVMSIRE
                     TIEALGGEYRFGTRVTDFIVEGEGRQLAGLELTDVASGAVSQLAARHVIMAVGHSARD
                     TFEVLHGRGVFMEAKPFAIGVRIEHPQSWIDKARYGVHAGNKVLGPAAYSLSHQASNG
                     RAVYSFCMCPGGRVVAATSEEGRVVTNGMSQYSRAEFNANSGLVVTIEPEKDYPGGPL
                     AGIAFQRHWESLAFVAGGSDYSAPGQKVGDLLAGRPSSGDFGAVEPSYQPGVKLTDLG
                     TCLPPYVMEAIREALPVFGRKIAGYDHPDVVMTGVETRTSCPLRITRGKDLQSLNTQG
                     LYPGGEGAGYAGGILSAAVDGIRLAEAVGAAILPQG"
     gene            26333..27388
                     /locus_tag="VFF98_11685"
     CDS             26333..27388
                     /locus_tag="VFF98_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZU64338.1"
                     /translation="MAGAGALALLGLGLGWRYETHGRYIEETNDAQLAADSVAISAKL
                     AGYVREVPVVDNDAVSRRAPLVKIDPVDYATRLDAANADIAAANAAQAAASAEAGQAR
                     AAADEAEAGLGEAQVELERAQREEGRYRPLASAGAEPAEKLAQLISNTAHARAEVALR
                     RATLARMRQQVALAQAHGQAATARAQSATTAAHAARNDLDATRLTAPIDGRVASLGVR
                     VGQFVSPGQRLMTLVPDGSLYVVANFKETQLGLMRPGQMAEIRVDALPGVVFHGEVES
                     TSPGTGAQFSLIPPQNATGNFTKIVQRVPVRIRIYAGPVSRRVLVPGLSLSVSVDTSA
                     GKEELAAIRAEQERRGQ"
     gene            27385..28950
                     /locus_tag="VFF98_11690"
     CDS             27385..28950
                     /locus_tag="VFF98_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU64339.1"
                     /translation="MSEAPSGTAPLETADLAAWLAVAAGTLGGFMAFLDVSVVNSAMP
                     VIQGEIGATQSEGTWISTAYLVAEIVVMPLTTWFDRLIGLRRFLLASAVLFTLFSMLC
                     GLAGDLPTMICGRVGQGLAGGSLIPTALTIIATRLPPRQQAIGIALFSCAALLGPIIG
                     PLLGGWITETYSWRYAFYINAPICGVLVALLLLGLPAKKGDWHELARADWAGIFGMAL
                     ALGSITILLEEGHREQWFDSALIRTLAAGCVVGCVAVGWGQWRSARPIVSLALLRNPA
                     LTATIVLSLMAGALMFTSLFSVPQFLAAIAGYNAWQSGTVIAISGGSAILAAALYPLV
                     IARVDVRLLVGAAFLVQALACWQASHLTAASDGTAFALSQMLLGVGLTLAVVPLQQVN
                     VSVVRLEEAGEASSLFVVARNLGASIGLAAVASFQDQRLEFHHWRLHSAIAASDPAMQ
                     AQVAADAAAHGGGADGLAAALTLVDSHVMMDALVMSFNDVFLALAVATLLASPLVLLL
                     RPIDPARAMGAAH"
     gene            28950..30329
                     /locus_tag="VFF98_11695"
     CDS             28950..30329
                     /locus_tag="VFF98_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU64340.1"
                     /translation="MRAPILVLGALALSACKLGPDYARPALPTGAAASLHAGEAPPPL
                     APWWPALADPRLDALEDEALAHAPTLAEARARVEAARAGLRAARAAQAPAMAVDAAYA
                     HAQLPGQFTTLTGIDRAEAYAAGLDASWEVDLWGARRRAVEQARAGAGLASASLADAE
                     VRLTAELAQTYVALAARQAAATLLEHRAGLEGQLLHMARLRLAGGTGTGQQVEQAAAT
                     LDATRADIALNAGEESALQAALAVLAGRAPGAAPAERIAAVPLPPARVEVGDAGALIA
                     RRPDVRMAERNLAAAHAGIGMALAQRYPSVSLLGFIGIGGLSAGHGFDSAQAGGLLLP
                     RLTWAFPDFGRVNAAVHAARAEDAAALAHYQAAMLGALQDADGALARFGAARSAYAGA
                     LDSEGHAHEAARLEALRAARGTIARSQALAAETQAISATLARQAAAARLTLAYAQLAK
                     ALGLGWQGP"
     gene            30326..30979
                     /locus_tag="VFF98_11700"
     CDS             30326..30979
                     /locus_tag="VFF98_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64341.1"
                     /translation="MSPAPPPPARGRPQAARAALIGHRIIEAAWGLLVDVGPEAFTID
                     ALALRARASKQTIYARFAGKQPLLAAVLASRLRRILAAVEEGGPAPSPLAHLADLAAR
                     AVLALSEPEARLFDRLIDWIDNAEPASTQPTGTQPTRRAVYAGFHAAAEGHLAHFCAR
                     WQCRLEDLPAVAAFWVEGLVGHVRCAITDADGHRDWAHAYAAHFLRAQGWAGNGRAD"
     gene            complement(30976..>32077)
                     /locus_tag="VFF98_11705"
     CDS             complement(30976..>32077)
                     /locus_tag="VFF98_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HZU64342.1"
                     /translation="EKPAAIAQGPAGPLPAARQPAAGEEGYEMVMLRYADVSEVVGLL
                     TDGLTVKSNDVFTPREPGFGSTSLTGGAYQASAPAMAGADDQPLGQSVDSAVAVDRRL
                     NAVWLRGSPAQIARMKAEIALIDVPVDSVILETQLVELTESGARSVGIDFTNANGQIG
                     LATVQTGEYIPSGFTANSGQRLTSAQLQAAVYAQVQKGEGRIVSRPRISARSGASAKI
                     ITGDALPILTSITLSGVNGVSQQVQYVNVGVTLQIAPRVSDDGFVTSHVFCVVSSVTG
                     YSSGYPTISQREAETSATVKDGESFVIGGLTQTSTISSDAKVPILGDVPLLGQAFHNK
                     KLTKAKTELYILVTPHIVRHAAPPAPLPEARP"
BASE COUNT         5036 a        11108 c        10819 g         5114 t
ORIGIN      
        1 gcacctcgcc ggcatgaagg acagcaaaac catcatcgcc atcaacaagg acgaagacgc
       61 acccatcttc caggtcgcag acatcggcct cgtcgccgac ctcttctcag ccgtgccgga
      121 aatcgccgcc aaggcctgag gaaaacagcc gcgcacgcac aggcgggccc cttcagggcc
      181 tgcctgctgc agccttgcgg cccctatccg cccagcaggg cgtaggtgag gccgaaaatg
      241 ccccaagcca ccaatgcccc cacgctgagg tggctcaacg ggcaattcac ccagatgatc
      301 tgcacctttt ccataaggcc tctccggcgc cctgttctgc ccaagggctg ccttgcccgg
      361 catgatacgc ctgttggcgc cgggttgaaa ggattcttcg ggccgccccc tcagtcatcg
      421 cacctctgat cgcgcctgcc gcccaaggat ggacaaatgc ccccggccaa gggaaacaga
      481 tgggcaggcg ggatgtgaaa ggcagcgatg catgacaggc gcaaagaagg cagccgcgaa
      541 cacggccgat cagccgatcg accatctcat cctgggtggc ggggcggcgg ccgtctcggc
      601 ggcgattgcc ctgcggctgg aagatccatc cgccaccatc acgctccttt gcgccgatga
      661 gggcctgccc ttctatcgcc cggcgctgtc caaacaggcg ctgctgggca ctgcggatga
      721 ggcgcagatc ctcatccacc ccgccagctt ctatgccgag cagaacatcc ggctggaact
      781 gggcacaacg gccatcgcgc tcgacaccga ggccagggtg gtgagctgcg ccagcggcgc
      841 gcgcttttcc tatcggcgcc tgttgatcgc caccggcgca aaggcgtgcc gtctggccct
      901 gcccggcgcc gatctgcccg gcatccatca cctgcgcgat cgggccgaat gcgcggccat
      961 gcgacgagaa atcgccgccg gcgcgcggcg ggcgatcgtg ctggggggca gtttccttgg
     1021 catggaaatc gccatgtcgc tccgcgatct ggggctggag gtaacggtga tcgagcagcg
     1081 cgaacaggtg ctgccgcatg tcgaatcggg ccgcgtttcc gatttcttcc gccgccatgc
     1141 cgaagagcgc ggcctcaagg tgctgttgtg cgacacgatc accgcgctgc atggcgtggg
     1201 ccggctgagc gaggtggaga cccgctccgg ccagcgcctg gcctgcgacc tgctggtggt
     1261 cagcatcggc gtggagccgg ccggcgcctt ccttgagggc agcggcatcg cctgcgcgca
     1321 agggctggtg ctggtggacg agcaattgcg caccaaccgc cccgatgtgt ttgccgccgg
     1381 cgacatcacc agcttttacg atcccgtgtt tggccggcgc cgccatatcg aacattggga
     1441 caatgccatc aagcagggca ggctggccgg gcgcaacatg ctgggccagc gcctgcgcta
     1501 cgatgaggtc tcctatttct tctgcgatgt gggcgatatc agcttcagca tgctgggcgc
     1561 gcccgaggag gcggatgagc ggatcgcccg cggcacgctg gcatcgcgct cgctggcgtt
     1621 gttctatctg cgcaacgatg tgccgcgcgc gctgctctcg gtgggccgcc cggccgagga
     1681 gacgcgcagc atcgaggggc tggtgcgcta tcgcgtgaac ctgcgcgcct gcaaggcccg
     1741 actcgctgat cctgcctttc cgctggaaca gatccccacg caaaccgtgc tggtgctgca
     1801 gggcggcggg gcgatggggg cgttcgaatg cggcgtggtg gaagcgctgg aggaagcggg
     1861 catcttcccc gatatcgtcg ccggcatctc gatcggcgcg ctgaacgggg cgatcgtggc
     1921 gggcaacccg cgcgatgcca gcgcggcgct ggtctccttc tggtcgcagc tggccatccc
     1981 ctcgcccgcc gcgctgatcg gcgaggaggc cgcccgcgcg ctcaccgcgg cgcagatcct
     2041 cgcctttggc gtgccgcatt tcttccgccc gcgctggatg cagccctttg ccgccagcct
     2101 gcccagcagc tggaccagct tctacgacat cggcccgatg aaggagctga tcgcccgcca
     2161 tgtggatttc gcgcggctga aggccagccc ggtgcggctg ctggtgggcg cggtcaacgt
     2221 cacttcgggc gagctggaag tgttcgacag ctataccgac gatctcaccc ccgatcatgt
     2281 gctggccagc ggcagcctgc cgccgggctt cccgtggact gagatcggcg gccaggccta
     2341 ttgggatggc ggggtggtca gcaattcgcc gctcgatatc gtgatggatc gctgcgggcc
     2401 ggatggcaag caggtctata tcgtcgatct gttcgccggg cgccggccgc tgcccaccaa
     2461 catgatggag gtgctggccc ggcgcgacga gatcgtctat tccgagcggg tgcgcagcga
     2521 cctgcgcctg cgcgagatgc agggcgccta tcgcgagctg gtggggcgga tgctggaact
     2581 ggtggatccg gccacgcgcg cgcgcatccg ccagcgcccg ctctatatcg agctgatggg
     2641 cgatggcgcg gccaccggca tcacccgctt catccgccag ggccgcgatg gcgagccctc
     2701 ctcgcgcgat tacgattttt ccgacatcgc catccgcgcc aaccgccagc agggcctggc
     2761 gctggccagg gccaccctcg ccgagcgcgc agaggcccct gcggccccgc gcgcggcggc
     2821 ggatgcgccc gcagccccgg cgcctgtgcc cctcaggcga gcagatgctg gccggaatcg
     2881 accggaatga tcgtgccggt gatccgcttt gcggcagggc tcaccagaaa ggcggccagc
     2941 gcgcccacat cggccacttc caccagctcc tgctcgggcg cgcgggcgcg ggcggcatcc
     3001 agcatctcgt cgaaccggtc tatcccgctg gccgcgcgtg tggcgatggg cccgggcgaa
     3061 atggcatggg cgcggatgcg tttgggcccc agctctgccg ccacatagcg ggtggtgctt
     3121 tccagcgccg ccttcaccgg gcccatcaga ttgtaatgtt ccaccacccg ttccgagcca
     3181 tagaaggtga ggcacagcag gcagccgcca tcggccatca gcggttcggc caggcgcgcc
     3241 atgcggatga agctgtggca ggagatgtcc atcgcctcgg caaagccctg ctggctgctg
     3301 tccaccaccc ggccgtgcag atcctcacgc ggggcaaagg cgatggaatg gagcacgaaa
     3361 tcaagcccgc cccagcgcgt gcctgcctcg gcgaacagcg cttccagctg gcccggcacc
     3421 cgcacatcgc agggcagcag ccattcgatc cccagccgct gggccaccgg ttccacaaag
     3481 gggcgcgcct tctcgttgag atagctggcg cccagccttg ccccgcactg gcggaaggcc
     3541 tcggcgcagc cggcggcgat ggaatgatca ttggcaatgc cgatcaccag gccgcgcttg
     3601 ccggccagat tgacgagcgg ttcggtcatc ggtctatcct cttcagcgtt catgcacata
     3661 gctgcccggc gccggggcca gcggcggaaa ttgcgcgctg cccatcgccg gcggggccac
     3721 cggcgcgccg gaatgcgccc ccagccactg cccccaggcc tcccaccagg atccctgatg
     3781 cgcctcggca tgcagcagcc attcctccgg gtcatagccc atctggcctt ccgcccgggt
     3841 cagcaggcga tagctgcgat gggggtggcc gggttccgac acgatgccgg cattgtgccc
     3901 gccgctggtc agcacgaaag tgatcggccc gtggctcagc tggtggatct tgtagaccga
     3961 gcgccagggg gccacatggt cgcgctcggt gcccacggca aagatcggcg cggtcacatc
     4021 ggccagcgag atgctgtggc catccacccg gtatttgtca tgcgccagct cgttttcgag
     4081 gaacaggcgg cgcagatatt cgctgtgcat cgcatagggc atgcgggtgc catcggcgtt
     4141 ccaagccatc aggtcgttca tcccctcgcg ctcgcccatc agataggtgg tgagcacgcg
     4201 cgaccagacg agatcgttgg agcgcaggat ctggaaggcg ccgcccatct ggctgctgtc
     4261 gagatagccg cggctccaca tcatgttttc cagcaggttg agctgggatt catcgatgaa
     4321 caggcccagc tcgcccggct cgctgaattc ggtctgggct gccagcaggg tcacgctggc
     4381 cagccgccgg tccccctcgc gcgccagcgc cgcggcggtg atcgccagca aggtgccgcc
     4441 caggcaatag cccagcgcat ggatggcgcc cgccccggtg atctcgccaa tcgcctccag
     4501 cgcggccagc gggcccagcc ggcgataggc ctccatatcg agattgcgat cagcgccggt
     4561 gggattgtgc caggaaatgg caaagacggt gtagccctgc cctaccagcc agcggatcag
     4621 cgaattgtcg ggcgagagat cgaggatgta atatttcatg atccacgccg gcacgatcag
     4681 caccggttcc ggccgcacgg tgccggtggt gggcgtatat tggatcagct cgatcagctc
     4741 gttgcgatag accacctgcc ccggcgttgc cgccacttcc tcgcccacgg caaagccatc
     4801 ggtgccggcg ggcggttcgc catgggccag ccggcccatg tcttccagca gatagtgcag
     4861 cccatccacc aggcagcgcc cgccggtttc ggcaatgcgc tgctgcagca ccgggttggt
     4921 ggcgatgaaa ttggtgggcg ccagcacatc cagcaactgg cgggcggcaa aggccaccat
     4981 gtcctcgtgg tgccgctcca ccccgcgcac gccggtggtg gccgcatgcc accattgctg
     5041 ggccatcagg aaggattgca ccaacaagtc gtagggcggt tgctgccagg cggggttgtc
     5101 gaagcggtgg tcttgcggca gcggctggat cgcctgcggt gccctcgccc cgcaggcgct
     5161 gcccagcaac tgttcggcaa tgcgggccca atcctgcgcg gcgcgggcgc ccagctgcat
     5221 ctgcttgccg ggcgaacagg ccagatgcag catccagtct gaacaggcct gcgccagcgt
     5281 gccgggcgaa aggccatggg tgagctgggc gatggcggcc accgctgcgc gatcgaaggt
     5341 ttcggccagg ccatccagcg gatcggcggc gggggtggca gcggattgcg gcatcatcct
     5401 gtctccttgg cccggcgggc agcccggcga gccagcccgc gcaatgtcgc atgctgagcc
     5461 gattcggcca cccccgacaa acgtaagagg cgattattcc agctgtttga caagcacctg
     5521 cgcacactcg gcaggcggat ttgacctggg cgccccatcc gcatataacc gataaacaat
     5581 cacattgtcc gatattcgtt caccagagga accgaaaatg cgatcgaaac tctacgccag
     5641 cccgcaagag gcgctggccg gcctgctgca tgacgggatg gtggtgatgt cgggcgggtt
     5701 tggcctgtcg ggcaatccgg aaagcctgat cccgcaaatc cgcgcggccg gcgtgaaggg
     5761 cctgacggtt atctcgaaca atgccggcgc cgatggcttt ggcctgtgga tgctgctgga
     5821 aagccggcag atcgcccgga tgatcagctc ctatgtgggc gagaacaagc tgttcgagca
     5881 gcaatatctc tcgggcgaac tggaactgga gctgaccccg caaggcacgc tggccgagcg
     5941 catccgcgcc ggcggcgccg gcatcccggc cttctacacc aaaacgggcg tgggcaccgt
     6001 ggtggcagaa ggcaagccca ccgagatctt cgagggcgag gaatatgtgc gcgagacctg
     6061 gctgcgcgcg gatctggcca tcatcaaggc ctggcgggcg gatccggccg gcaacctcat
     6121 gttccgcaag accgcgcgca acttcaaccc caacatggcc accgccgcca aaatcaccgt
     6181 ggtggaggtg gaggagatcg tgcccgaggg caccttcgat cccgactgca tccacacgcc
     6241 gggcatctat gtggaccgga tcgtgctttc caccatcaac gaaaagcgca tcgaaaagct
     6301 gacaacccgc gtgaaggagc ccgcccaatg agctggaatc gcgatgaaat ggcggcccgt
     6361 gcggcgcagg aactgcaaga tggcttctat gtgaacctgg gcatcggcat ccccacgctg
     6421 gtggccaacc atgtgccggt cggcatcacc gtcacgctgc aatcggaaaa cggcatgctg
     6481 ggcatcggcc cctttcccta tgagggcgag gccgaccccg atctcatcaa cgccggcaag
     6541 cagacggtga cggcgctgcc caccagcagc ttcttttcca gcgccgacag tttcgcgatg
     6601 atccgcggcg gccatatcga catggccgtg ctgggggcaa tggaagtggc cgccaatggc
     6661 gatcttgcca actggacgat cccgggcaag atggtgaagg gcatgggcgg cgccatggat
     6721 ctggtggcgg gcgtgaggcg catcgtggtg gtgatggacc actgcgccaa ggatggcagc
     6781 cccaagatcc tgccggcctg caccctgccg ctcaccggcc ggggcgtggt cgatctcatc
     6841 atcactgatc tggccgttat cgcggtggac aggaaggccg gcgggctgac gctggtggaa
     6901 accgcgccgg gcgtcagcgt ggacgaggtg gtggccaaga ccggcgcgcc gctggccatt
     6961 actcagggag ccgcatgatg gccgaggcgt tcatctgcga tgccattcgc acccccatcg
     7021 gccgcctcaa cggcagcctg gccaccatcc gcgccgatga tctggccgcc ctcccgctgc
     7081 gggcgctgat ggcgcgcaac ccgggcgccg actgggccgc gctcgacgat atcctgctgg
     7141 gctgcgccaa ccagtctggc gaggataacc gcaaccttgc ccgcatggcc ggcctgctct
     7201 cgggcctgcc caaggacgtg cccggcgcca ccatcaaccg cctgtgcgga tcggggctga
     7261 atgccctggg gctggccgcg caggcaatcc gcagcggcga tgccgatttc ctgatcgccg
     7321 gcggcgccga aagcatgacc cgcgccccct tcgtgatggg caaggccgcc agcgccttcg
     7381 cgcgtgacca gaagctggaa gacacgacga tgggctggcg cttcgtcaac ccggcgatga
     7441 aggccgcctg gggcgtcgac accatgccgc agacggccga gaatgtggcc gccgaatggg
     7501 gcatcagccg cgccgcgcaa gatgccttcg cgctggccag ccaggagaag gccgccgccg
     7561 cccaggccag cggccggctc gccgccgaga tcctgccggt ggacgtgccg cagaagaagg
     7621 gcgcgccgct tgtcttcgcg gcggatgaac acccccgcgc caccagcctt gaagcgctcg
     7681 cccggctgaa gccagtggtg catgccgatg gcacgatcac cgccggcaat gccagcggcc
     7741 tcaacgatgg cgcggcggca ctggtgatcg ccagcgaggc ggccgcgcgc gcccatgggc
     7801 tgaccccgcg cgcccgcgtg gtggcgatgg ccagcgccgg cgttgccccc cgggtgatgg
     7861 gcatcggccc ggtggaggcc acccgccgcc tgctggcccg caccggcctc tcgctggccg
     7921 ctatcggcgt gatcgagctg aacgaggcct ttgccgccca ggcgctggcc tgcctgcagg
     7981 atctcggcat cgatccggcc gatccgcgcg tcaaccccaa tggcggggcg atcgcgctgg
     8041 gccatccgct gggcatgtcg ggcgcacgca ttgcgctcag cgccgtggag gagctgcagc
     8101 gcagcagcgc ccgccatgcg ctggccacca tgtgcattgg cgtgggccag ggcattgccg
     8161 tgctgctgga gcgggtctga accaaccact cgacagggcg cgcgggcgat gccatggcaa
     8221 gcggggtgag cacatccgat cccgatttca tgcaatcgct cgcgcgcggg ctccaggtgc
     8281 tggaagcctt tgccgaactg ggcccggggc aaaccatcgc cagcctggcc cgccacaccg
     8341 ggctggcccg cggggtggtg gcgcgctgcc tgcacacgct ggcggccacc ggctatgtgg
     8401 agcaggccgg tcgccagttt ggcgtgcgcg gcaaggtgct gcggctggcc gaggggtttc
     8461 tggccgatgg ctcgctgccg gcgctggccc agccgctgct ggaacagctg cgtgacgagc
     8521 tgggcgaaag ctgctcgctg ggcgtgctgg atggggcgca ggtgctttat gtggcgcgcg
     8581 cctcgcgcag ccggatcatg gcggtggggc tgcatgtggg cagccgcttg ccggcctggt
     8641 gcacgtccat gggccgggtg ctgctggccg ccctgcccgc agcgcagcgg gccgcgctga
     8701 tccccgccac gctgcccgcc cacaccgccc acacgatcac cgatcccgcc cggctggccg
     8761 ccgtgctgga cgaggtggcg cacagcggca tggcgctggt cgatcaggag ctggaagtgg
     8821 gcctgcgctc ggtagcggtg ccggtgcgcg atgggcgggg gcaggtggtg gccgcgctca
     8881 acatgggcgt caacgcgctg gcgcatgagc cggaggcgct gcgccagacc atcgccccgc
     8941 gcctgcgcca ggcggcagag cggctgggcg ccagcctgcc atcaagggat tagggcgcaa
     9001 aaaaggggca agggcccatg gcccctgccc cccttttctc aggccccaga ggaccttaac
     9061 ccatgccggg cctcagccca tcgtggggat cacgaaagcg ttgccgccat cggcgctgcc
     9121 atcgggccag cgggcggtca cggtcttcac cttggtccag aacttcacgc cttccatgcc
     9181 gtgctggttg gtgtcgccaa aggcgctgcg cttccagccg ccaaaggtgt gataggccac
     9241 cggcaccggg atcggcacgt tgatgcccac catgcccaca ttcacgcggg cggcaaattc
     9301 acgcgcggca tggccgttgc gggtaaagat ggccacgcca ttgccatact ggtgcttgct
     9361 gggcagggcc acggcggtct cgaaatcggc cgcgcgcacg atctgcagca cggggccgaa
     9421 gatctcgttg tggtagctgt ccatctcggg cgtcacatgg tcgatcagcg tcgggccgac
     9481 gaagaagccg ttctcgtggc cctgcagctt gaagccgcgg ccatcgacga ggatcttgcc
     9541 gccttcggcc tcgcatttgg cgatccagtc ctcgatccgc gccttgtgct gggcggtgac
     9601 gaccgggcca taatgcgccg ccggatcgtt ggagacgccc acctgcaggc tctcgatctc
     9661 gggcagcagc cggtcgatca gccgctcggc cgtgccctcg cccaccggca ccaccaccgg
     9721 cagcgccatg cagcgctcgc ccgccgagcc aaaggccgca ccgacgagat cgttcaccac
     9781 cttgtcgaga tcggcatcgg gcatgacgat gccatggttc ttggcaccgc ccatcgcctg
     9841 cacgcgcttg ccattggcag cgccgcgctc atagacgtaa ttggcgatgt cggaggagcc
     9901 gacaaagctg atcgcgccga tcgccggatg atcgaggatg gcgtccacca tttccttgtc
     9961 gccatgcacg cattgcagca cgccttcggg ggcgcctgct tccagcatca gctgcgtcag
    10021 gcgcaccggc accgagggat cgcgctcgga aggcttgagg atgaaggcat tgccgcaagc
    10081 gatcgccacg ccaaacatcc acatcgggat catcgcgggg aagttgaacg gcgtgatgcc
    10141 cgcgccaatg ccgatcggct ggcgcatgga gtaaacatcg atgcccgggc ccgcgccatg
    10201 ggtatattcg cccttcagcg catgggggat gccgcaggca aattccacca cttccagccc
    10261 gcgctggata tcgcccttgg catcggccac caccttgcca tgctcgctgg ccagcagctc
    10321 ggccagctcc tgcatgttgg cctcgaccaa gcgcttgaac tcgaacatca cgcgggcgcg
    10381 gcgctgcggg ttggtggccg cccaggccgg ctgcaccgcc agcgccttgg ccaccgccgc
    10441 atccagcagc gcggcatcgc ccagcgccac ttccgcctga accgcgcccg tgttggggtc
    10501 gaacaccggg ctcttgcggg ccggcacacc gccgccgccc acgatcacat ggtcaatcag
    10561 tcgcatttcg cttcaactcc gttcaggcat ccttgatttg cacccagcat tatcccctcg
    10621 aaaattgcgg gcaactctca ataaagctga tatgacctgc aaatatgcag gatgccgact
    10681 ggagcgatta tcaggccttt ctcgccatag cccgcgcggg ccagctggcg ggcgcgggcc
    10741 gcatgatggg gctgaatgcc accaccatcg gccggcggct gcggcggctg gaaagccggc
    10801 tgggcaccac cctgttcgag cagacccgcg agggccagct gctgacaccg gccggcgagg
    10861 cgctgctggc cgccgtggag cagatggccc aggccgccag cggcgtggag ccggccagca
    10921 gcgccaacgg ccccagcggc agcgtgcgca tcagcgtgtc ggaagggttt ggcagctgga
    10981 tcgtggcgcg ccatctgggc gattttgcca agcgctatcc ggggctggcg atcgatctgg
    11041 tggcctccaa cggcttcctc agcccctcgc gccgcgaggc ggatatcgcc gtgctgctct
    11101 cgcgcccgca gacagggccg ctgatcgccc gcaagctggc cgattatgcg ctgcgcctct
    11161 atgccagccc cgcctatctg gccgggcacg cggcgatcac cggcccggcc tgccttgccc
    11221 atggccacca gctgatcggc tatatccccg atctcgtcta tgcgcccgag ctgaactatc
    11281 tgaccgagat cgcccccgat ctggccgcga gcctgcgctc ctccagcatc ctggcccagc
    11341 accggctgct ggcggcgggc gccggcattg gcgtgctgcc ctgcttcatc ggcgatgccg
    11401 atccggcgct ggtgcgcgtg ctgcccgacc gcaccatcac ccgcgccttc tggctggtga
    11461 cgcacaagga cacccaccag ctgcagcgca tccgcctcgt cacccgctgg ctggccgata
    11521 tcgtgaaggc cgatgcccgg ctgctggcgc cttgagggca ccacagccgc gccggcgcca
    11581 tcgataggcc gctatccaca gcatgggctg gaacgccgcc ccgccccgtg ccagccttgc
    11641 cggcaaaagc cagaccgaag ggagacacac ttggccgctc accccacccc atcgccccgc
    11701 ctggcaggca ggatctagcg atggccgcat tcaccttcca ccacggcggc gtgtccgtgc
    11761 ccagcctcga tgaggcgatc gcctggtatg gccatgtgct gggctttgaa ctggagcggc
    11821 gcttcttcat cgaggcggcc ggcagccaca ccgccatggt gcgcaaaggc ccgctgcgct
    11881 tcgagatctt cgaagtggca ggcgcggccc ccctgcccga agaccgccgc caccccccgc
    11941 gcgacatcaa gacccatggc aacaagcatg tcgccttccg cgtggccgat atcgaggcgt
    12001 ttctcgtcga gatggaagaa aagggcgccg atatcgcctt tgtcgtgcgc gaggcgttcg
    12061 gcaagggctg cttcatccgc gattgcgcag gcaatctgat cgaattcgtc gaagagcccg
    12121 aaggctgaaa aaagggggca ggtgccaggg gctgccccct cgcacctgcc ctttgcctta
    12181 caccggttcg gccagcttgg cgcggatgcg tgcagtcagc tccaccgggt cgccagcggt
    12241 ttccgggtgc atttcccact cgcccagctg gatagagctg gtgcccacca gcttggcccg
    12301 ctcgaagcgg cgctgcatga aggccgcaag cgcctcatcc agatcgcgcg tggccagctc
    12361 ctctgccagc accaccgcat cttccaccgc catcgccgcg ccctggccca gatggggcgt
    12421 ggccgaatgg gcagcatcgc cgatcagcac cacccggccc tgataccagg gggcgggcat
    12481 gatgcaggct tccagcgggc gatagaccac cagcgccgga tcgatcaccg ccttggccgc
    12541 ctcgcccagc aggccgccat agccggccag gcgcttgcgc atttcctcgg ccagcgtctc
    12601 gggcgcgaag cgcggattgc ccttctcctc gatgaccgcg aagatataca tctcgctcgg
    12661 cgtcagcggg caatagccgg ccttgccgtt gaaaccgttc tggccattgc cgatgtagat
    12721 gtcggaccat tcgagatcag ccggccgcgg cacgttgtag cgccacacgc cctggccggt
    12781 gaactgcggc cgggcatcgg gccacaacgc cttgcgcacg gccgaataat tgccatcggc
    12841 gccgatcagc aggtcgtagt ggccggtggt gccatccgag aaggccacgt ccaccccatc
    12901 gcccttgtcc tcgaaggcgg tgaaggtaac gcccaggcgc accgcaatgc ccagctcgcg
    12961 caccttttgc gtcaacacct catgcagcgc cgggcgggtg aggccaagat cgcagggcag
    13021 gccatcgacc tgcgcatcgc cgggcagttc ggcaatggtg ttgcccgccg catccttgaa
    13081 gcgcgcgccc ttgtaggggt agcccgccgc caccgccgca tcgcccagcc ccagttcggc
    13141 cagcgcgcgg atgaaattgg cctgcacgat gatgccgaca tgatagacct tccagtcctt
    13201 gtcgcgctcc accacttcca ccgccaggcc ccgctgatgc aggccaatgg ccagggccaa
    13261 accgcccacg ccgccgccga tgatgcagat cttctgtgcc tttgccatgc tcgctctcct
    13321 tattcggccg cttcggcggc aatgtctgtg gcaggaacgc ctgcgggtgt agtgttggcc
    13381 ggtttggggt ctgccggcag gaagcgcagc tgctcgtcgc gcgcataggt cacgtcccag
    13441 ttgggcgtgg gcatcatcca ttgcggcaga tcatgcatga agatcgccgt gccaaaggcg
    13501 ccatcccaca cgcgcggctt ccagctgtcg tccacgcagt cgcaatcggc gccatattcc
    13561 atgtcgccgc ccagcgccgg catgttgaga tagaggaagg cggcgctggt cagccggtgg
    13621 cggccaaggc cccagttgcc ggcaaaatca tagcccagcc ggtcgaggtg gttcttgccc
    13681 accatgatct cgtcgatatc ctccagctcg aaattggcgt ggtggaattt cagcgtgcca
    13741 tcaaagccgg gcatcggcgc ctgggcgttg gccagaaaga tgttgtggtg ctcgaaagcg
    13801 gcgctggcgc gcatgtagac gccaaagccc ttctgcacgt cggtgatgcg aaaacccagc
    13861 cggtcgcggt agtattccag cgcttcctgc acatcgggga aggtccacac ggcgtggtgg
    13921 atgcgctttg gcgtggcacg ggccagccat ttgcgcggct ggttcagccg gccgacaagg
    13981 cccggcgtgt tggccgggct gggcgcgcca tgcaccgcgc gcggcgtcca gtgctgcagc
    14041 gccaccgcct ggccgaaggg ggtgacgaag tggacaacgc cctcggcatc ggtggccagc
    14101 tgatgatcgc gggcaagatc gtcggtcagc cgcttcaggg cggcggcatt gtccacgctc
    14161 cacacgcatt ccaccacgcc aatgccggtt tgcgcatggc cctgcggcag atccgggtgg
    14221 ccgcgcagca gcagcagcac ctcggcgccg ttcatcacct cgaagcggct ggaagtggca
    14281 tcgcccgcga tgaagctgag gccataatcg gtgaaaaagc gtgtggtggt ggtgaggtca
    14341 tcgaccagaa acaccagccg gcgaatgcct gtgattgcca tttcctgcat ctcccttgcg
    14401 tgttgcgcaa ggaatggcgc gaggcggccg ccggctggtt gatcgcgatc aattctgatt
    14461 tatggaaatt accgctgcag ttgcatcggt ttcatggatg gataaaccgc ccctcctcag
    14521 cgccggaaca agagcgtgac gaggaacaga atggtcttgg catccagcag tttaagcgcg
    14581 gcgcgcgctt ctgccggggt caatttggcc accatcggca tggcgccgaa gatcttgccg
    14641 cggatgacca gcgcatcacc ctcgcgctca atcttgcgca cggtcatcag ttccttgtcg
    14701 ttggcatcga gcacgcgcat ctgcggcggc ttgctattct ggctcatcag atcgcccctc
    14761 cacacacctt gtcgaaatcg atacccatgt cgtccatcat ctggatggcc gtggcgcgcc
    14821 cggcgccaaa cacgccgccg cccggatgca tgaacggccc gacgagatag agcccttcca
    14881 cctgcggcac gcggaaagag ccgagatcgg gcgtgggccg gtggccgaca gactgataca
    14941 tgaagggcgc gcagccgtgg atatcgccct tgaggaagct ggcggggctg tcgcgctcat
    15001 gatcgatggg cgagcggatg aggcgaccgg tgatgttgtc gtcggtcagg ttcgcataga
    15061 acttgcggta ttgggccagg gccgcatcgg ccacttcttc cttcttctcg tcccacgcgg
    15121 catggccgcc ctcgaacagt tcgtagggcg cgaaattgac gccatagaac acgcccgccc
    15181 cttccggcgc ccggctggga tcgaagatgg tgttgtcacc cccggcgatc agccgcggcg
    15241 cggtgaggcc ccggcgcagc ttgtcatatt ccagcagcat ctcgcggatg gtgtagaagt
    15301 ccatcagctc ggtcatcatg gcattggttt cattgcccac cttgagcctc agccgctcct
    15361 tcaaggtgag atgggtgagg ttgatcgaga aggtggacgg ggtgacgcgc tcggcccgct
    15421 gcagcacggg ctcgggcgtt tcggccacga atttgcgcag cacatggggg tggatggcgc
    15481 cgatcacgcc gtctttcgcc atgaaggttt cgccggtggt cagctccagc ccggccgcct
    15541 tgccgcccga gacgatcacg cgcgccacct cggcattgca gcgcacctcg ccgccccaat
    15601 gctcgatcgc gcgcaccagc gccttcgaca gctggcccga gccgcccttg ggcatcgagc
    15661 agccataggt gtggatgatg cccggcatca ggaaggcgcc catgccggtg cccagctcgt
    15721 cgggcatctg caggttttcg gtcaccatcc gcacgatgtg aagcttgagg agatcgctct
    15781 cgaaatactg ctccaccaca tcgagcgccg agcggctcat cacatcgagc aggaagcggc
    15841 cctcgtcgga ctggtccatc atcgccacaa aggcgcccat cgggaagggc ggcatgtaga
    15901 gccccgacat gaacatcggc agggcggcca tgctggccct ggcaaagccg cgataggcct
    15961 cggcatcctt ggggctgaag ctggcgatct cctcgcaggt gcgatccaga tccttccagc
    16021 tgcgcacgat cgtgccatct tcccagatcg aggcatgcgg cagatcggga tagatatatt
    16081 ccaggccaaa gcgcgaaagc aggcccagtt cgtcctcgcg cagcatgggg ttaccctgga
    16141 tcatgatatg cacgttggag tgctcgtcat gccagaagcc gggctggatc agctcgcgcg
    16201 tggaaacgcc gccgccgaaa tggggcttcc tttccagcac cagcaccttc ttgccggcct
    16261 tggccatata ggcggcggcg gtgaggccat tgtggcctgc gcccatcacc acgatgtcat
    16321 agttgctcaa tggttctctc ccgaaaagaa ggcgcggaac ggggccaggc ccggcacgtg
    16381 cgaggcgcag ccgccatcgg ccaccagcac ctggccggtg atgttgcgcg ccgcatcgct
    16441 ggccaggaag gccaccgcct cggcaatgtc ttccggctcg cccagctggg tgcgcagggt
    16501 ttcgccctcc accgcggcgc gcaaaggcgg cgggaaggcc tcgcgcaggg ccggcgtcat
    16561 cgtcatgccc ggcgccaccg cgttgcagcg cacgccatgg atgccgtggc tggccgcgat
    16621 cgcccgggtc atctggatga tcgccgcctt gctgctggaa taggccgcct ggatgatatg
    16681 cccctgcagc gccagattgc tgacggtgtt gacgatattg ccatgcgtgg cgcgcaggtg
    16741 cggcagcgcc gcccggcagc cgatcatcgt gccgcgcaca ttcaccgcat aggtgcggtc
    16801 ccagagggcg gtgtccatcg cttccacatc gccatcggcg cgggcgattt ccgggctcag
    16861 cagggcggca ttgttgtgca gcacatccag ctggccgaaa tggctgaggg tggccgccac
    16921 catcgccaga atgctctctt cgcgctccag atccagccgg atcgccagcg cgtccggcag
    16981 ttcggcggcc agggcctcgg cgcgttcgaa ggcaatgtcg gcaatcgcca ccttgccacc
    17041 gcgcgccgcg atcagccgcg cggtggcagc accaatgccg ccagccccgc cggtgatgag
    17101 cgcaaccttg ccgtccatgc ggatggcgtc ttgtgccatc ttgtctctcc ccttgaattg
    17161 gtttgtgagg cagggtttaa ggcggcgctg cgcaagcgga aagacggcac ttttttgtgc
    17221 cctcgccgcc ccatggaaca ggcaatgaca gacggtatcc cgcaacatct gggcgcgcgc
    17281 atcgcagcgc tggccgaaga gatggcccgc cacggcaacc accgcgaggc gcccgtgcgc
    17341 gaggtgatgg ccaagctggg cgacaggtgg tccacgctca tcctcatcgt gctggaaatg
    17401 ggcgagtggc gccatgccga tctgaagcgc gtgctggtgc aactgtcggc ggaaaaggcg
    17461 atttcgcagc gggtgctgac gctgaaactg cgcgcgctgg aacgcgatgg cttcgttgag
    17521 cggcgggcca ccgcggatgt gccgccgcgc gtatcctatc ggctgacggc actgggcgaa
    17581 aggctggcgg ccgaggcgcg gcggatgatc ggctgggtga acagccaggc cggcgccatc
    17641 gaggcggccc gccaggcctt cgacatgcgg gaggactgag gcgggagccc atcctcccgc
    17701 gcccctcagc gcccgctgtt cagctgctgg aactgggcgg cgatggcatc gcggtgcact
    17761 tcggccagcg gcgcgatcgg caccacattg ccattgccgc gcgcggccgg cccgccttcc
    17821 tgcgcatagg ccgacagccg ctccaccacc cagcgcaggg cccggtcgtt gttgttcgac
    17881 aggtgccact ggatcgcctc gcggatgggc ggaatgtcga acggcatctc gcgcatcacc
    17941 agcggcaggt tcttggtcac ctgggtggcc aggcggcggt gcatggtggc cacgcgctgc
    18001 gtgcccagca ccagcccggg cagcgagagg aagctgggcg ccaccacctc gatgcggcgc
    18061 tgggttttct ggcggcgcac gaaccagtcg tcgaaagcgg gggtgcgggc gcggccgaag
    18121 cgcgccgtca catggcccag ggcgaaatat ttctcgcggg tcatgtcgcc ggccatcgcc
    18181 gggttggcgg catcgcccac caccacgtaa tcatcctcga acaacagctg gctgggatga
    18241 tcggcgctga tcgcatagtc gatcgtcagc agcaggtcga tctggccgcg ctccagcgct
    18301 tccaccacat tgtcgctcat cggctggatc tcgaagcgca ggtgcggcgc ttccttttcc
    18361 agcatcacca gcgcgccggc cagcagcacc tgggtggaat agtccgacgc catgatacgc
    18421 accagccggt cggccgtggc cggatcgaag gccggggcaa tggcgatggt ggtgcggatc
    18481 tgctcgagca cggtgcgcac cggctcgatc agttcctcgg cgcgggcggt gagcaccatg
    18541 ttgcgcccct tcaccaccag caattcatcg ccgaaatagt cgcgcaggcg gcccagcgcg
    18601 ctcgatgtgg ccgattgcga caggcacagc ctgtctgccg ccagcgacac gctcttttcc
    18661 gacagcagcg catcaagcgc caccagcaga ttgagatcca gcctttggaa ccgcatgagg
    18721 ccaacctttc cctattccag cgtctgaagc gcgctggcaa caagggtagc aagatcgcca
    18781 tcatgggcaa agcccgcttt ctcggcggcg ggcgtggaaa gcggcggctg tgccccgaaa
    18841 gccacttcca gcgccgcatc gggccgatag ctgaccagat ccgtgcccac gccgcactgg
    18901 cgggcgatct cggccgccat atcgcccatg gtcacgcgca gcgccggcag ggtgacagcg
    18961 cgggtgggcg gcagcagcgc ggtatccagc gtgagcgcgt ggaggaaatt ggccgcgcag
    19021 cgcgccaccg attgcgccca gatcgtggcg ccggccgaaa ccgggcaggt gaacgcctca
    19081 ccggcggcca gcgcgtggaa caggtcgctc atgaaggcgc ttttcatgcc gctggggccc
    19141 ttggggcggg cgaggatccc cggcaggcgc accgtcaccc cttccagcca gccgcgattg
    19201 gcaaacagcg ccaccgcatc ttccatcatc gccttgtggc cgccatagat catcttgggg
    19261 ctgagcgggc tggcatcatc gacaaaggcc ggcagcggat cgccgaacac cgcgatggaa
    19321 ctggcataga caaagcgcgg cacggtgccg cccggcccct tgcgggcagc gacatcctcc
    19381 atcaggtcat acatcgcatc gaggttgatg cggcgcgagg cggcagggtc ggcctcggca
    19441 gcgccgccgg gcacggtggc cagatggata acgccggcaa cgccgcccgc cagcgccggc
    19501 gcggcaaggg cggccgcgcg cacatcggca tcagccagat cgccagcgat agccagaagg
    19561 ccagcgccgg tttccagcgc cgccacatcg cggtccatcg ccaccacatc atgaccctgc
    19621 gccagcagcc ccgccacgat ccgccgcccg acaaagccgg cagcgccggt cacgataatg
    19681 cccatcttgc tctccccggc cgcgcagccc ttgcatgggg cgctgcggcc acgtctgttt
    19741 ccagcctgcc gcctagcgcg cgccccctcg cctgtctcac ccattgtgta gatagcagct
    19801 tatctacgct tcgggtttga atggcgcggc caccgcttgt taggtggggc gagaagattt
    19861 ctagggagaa tgcaatggaa gggctgcgca cgctgatcgt gggcgggggg attggcggca
    19921 tggcggcagc gatcgcattg gccgaacgcg gcgcggccgt ggaactgatc gacctcgacc
    19981 ccaactggcg cgtctatggc gcgggcatca ccatcaccgg gcccacgctg cgcgcctatc
    20041 gccggctggg cctgctggac cagatcaagg cgcaaggggc gatcactacc aagacccgcc
    20101 tgttccgcta tgatggcacc cacatcaagg atctcgacga gccggtgatc gaggaagggc
    20161 tgccggccac cggcggcatc atgcggcccg tgctgcaccg gatcatgcag gcgcgcgtgc
    20221 aggaactggc gatcccggtg cgcctgggcc tcaccgtgac ggcgctggaa aacaccggcg
    20281 cgggcgtcga tgtcaccttc tcggatggca gccagggcca ttatgcgctg gtggtgggcg
    20341 ccgacagcgt gttctccacc gtgcgcgaga tcgccttccc gcacatgggc gcggcccagc
    20401 ccaccggcca gggctgctgg cgcatttcca tcgccaagcc gccggggctg gaatatggcg
    20461 agttctatct gggccatgac aacccctgcg gcatcaccgc ctgcgcgccc gacagcgtct
    20521 atatgtggat gctcacccgc catgtggagc gcgagacctt cctcaccgag gccgagctct
    20581 atgacgagct gaagcgccag ctggccgatt ttggcggcaa tgccggctgg atccgcgacc
    20641 atatgacgat ggacgactgg atcaactatc gcccgctggc ggcggtgttg cagccggcgc
    20701 cctggtctgt cgggcggatc gtgctgctgg gcgatgcggt gcatgccacc accccccacc
    20761 tcgcctcggg cgcgggcatg gcggtggaaa gcggcatcgt gctggccgag gagctggagc
    20821 gggccgacag cgtggaagcg gcgctcgccg cctatgaggc gcggcgcttc gatcgctgcc
    20881 gcgatgtcat cgaatcgagc gtgaagatcg gccagctgca gctcgatcat gcctcgcccc
    20941 aggcccatgc cgaagtgctg gaaggggcgc tggcccggct gaaccagccc ttctgagcca
    21001 ggctgcggga gagcgggccg gccggcccct cttccgcagc gccggccggc tgcggccggg
    21061 cgccggcaaa gcttgtcttg ctgcgccgca gcgtgtatat ggctggccaa ggccaaggtg
    21121 gtggcaattg ttcaccgccg ggccgggcag cgtgagggcg cggttttggc catgcccgcc
    21181 cggcctttgc acaggccctt ctgccatatc cggaatgcgc ctttcacgcg gggttgcctt
    21241 catccatccc cgctccggcg cgcggcctcg cgtgccggcg cccgctgtga aagacgctca
    21301 tgtcctattt ttccgatctt ggccttgccg aaccgatcct gcgcgcactg gccgaaaagg
    21361 gctatgccga tcccaccccg atccagcgcc aggcgatccc cgcgctgatc gagggccgcg
    21421 atatcctcgg cctggcgcag accggcaccg gcaagaccgc tgccttcgcc ctgccctcgc
    21481 tgcaccgcct ggcggccgat cgcaagccga agaagccggc ttcctgccgc atgctggtgc
    21541 tctcgcccac gcgtgagctg gccgcgcaga ttgccgagaa catgcgcttt tatgccaagc
    21601 acctgccgct gagcgtgcaa tgcgtgtttg gcggcgtgcc ggcgcccaag caggcccgcg
    21661 cgctggtgcc cggctgcgac attctggtgg ccacccccgg ccgcctgctc gacctgatcg
    21721 acagccgcgc gctgaccctg cgcgatgtcg agatcttcgt gctcgacgag gctgaccaga
    21781 tgatggacct gggcttcatc gtgccgctga agcgcgtggc ccgcctgctg ccgcagcagc
    21841 gccagagcct gttcttctcg gccaccatgc cgcaggcgat cgaggaactg ggcaagcagt
    21901 tcatcaacaa ccccgtgaag gtggaagtgg ccccgcaatc caccaccgcc gagcgggtgg
    21961 agcagaacgc catcttcgtc agccagccgg aaaagcaggc gctgatgacg atcatcctgc
    22021 gcaacggcct ggcctcgaac gagatcgacc gctgcctcgt cttctgccgc accaagcatg
    22081 gcgccgatcg catcgtgcgc ttcctcacgc ccgccggcat cccggccgcc gccatccatg
    22141 gcgacaagtc gcaggcccag cgcaccgccg cgctgaaggg cttccgcgag ggcaatgtgc
    22201 gcgtgctggt ggccaccgac attgccgcgc gcggcatcga tgtgaccggc gtgtcgcatg
    22261 tgttcaactt tgacctgccc aatgtggccg agcaatatgt ccaccgcatt ggccgcaccg
    22321 cgcgcgccgg cgccgatggc attgccctgt cgctggtggc tgaggacgag cggccctatc
    22381 tgaaggccat cgagcggctg accggcgtga agctggccct gcagcccctg cccgaggatt
    22441 tcctcaagga agcctcgcgc ctgcccgccc ctgccaagcg cgcccgtggt ggcgaaggca
    22501 atggcggccg cggcgagggc gcgcgcggcg ggcgtgatgg cgggcgcgat ggcggccggg
    22561 gccagggcgg cggcggccgc agtgaaggcg gccgcagcga aggcggccag cgtgatggtg
    22621 ggcagcgtga tggcggccgg ggcgcccggc ccgaggaagc ccgcgccaag cgccgctttg
    22681 gcgaaggccg cgaggagcgc gtgcgtgatg gccagcgctc gtccggccag cgcgagaacg
    22741 accgcgcccg cgccgaacgc cgccagaccg agcgccgcgc cggcgaacag cgcgccgacc
    22801 agcgcagcgc ccgcccctcg gcccgcagca atttcgatcc gctgcgcgaa aacaccgatg
    22861 ccccggcccg ccgccagggc caaggccgcc ggggctgagg ccctaacctc tggaaaaccc
    22921 gcgggggccc cctagccggc gcccccgcca ctggctaacc ctgccggcgt gagcgcatcc
    22981 atcatcattg gcgaggattc caagggcagc cccttgcgga tcgatatcga ggaattgctg
    23041 gccacgcgcc ttctggtgca gggcaattcc ggctcgggca agtcgcatct gctgcgccgg
    23101 ctgctggagg aatcggccgc cctggtgcag caggtggtgg tggatccgga aggcgatttc
    23161 gtcacgctgg cagagccttt cggccatatc gtggtggatg gcgcggccta taccacggcc
    23221 gagctggtgc gcctggccaa tcgcgtgcgc cagcatcgcg ccagcgtggt gctggccctc
    23281 gacgggctgg agatcgaggc gcagatgcgc tgcgccgcca ctttcctcaa caccctgttc
    23341 gatgccccgc gcgagcaatg gtatcccgcg ctggtagtgg tggacgaggc gcagatgttc
    23401 gcgcccgctg ctgccggcga tgtgtcggac gaggtgcgcc gcctgtcgct gagcgcgatg
    23461 accaacctca tgtgccgcgg gcgcaagcgc ggccttgccg gcatcatcgc cacccagcgg
    23521 ctggccaagc tggccaagaa tgtggcggca gaggccagca atttcctcat gggccgcacc
    23581 ttcctcgata tcgacatggc ccgcgcggca gaccttctgg gcatggaccg gcgccaggcc
    23641 gaaaccatcc gcgatctggc gcgcggccag tttctggcgc tgggcccggc gatcacgcgg
    23701 cggccggtgt cagtgcggat cggcggggtg cgcaccaaga gccaggcgat ctccaccggc
    23761 ctgctgccgc cgcccgaagt gaccggcgag gacctccacg cgctgctcca cgctgccccc
    23821 agcgaggagg aagcggcgct ggccgaggcg cccgcccgcc tgctgccgcg cgccgatgcc
    23881 ggcacgctga tcgatcagat ctccagcttt gccatggccg ctgcccccgg cgaggagccg
    23941 ctgcccgcca gccccggcga tgatctgttt gccgcggcca gcgtgcccgc cccgcccagc
    24001 ctgccgcccg aggaagtgca ggcgatcctg gccgagatca tggccgatat cgccaccgag
    24061 cccgattgca ccttccagcc cgccgccagc ctgttccagg atttctcggt gcgctgccgc
    24121 atgcgccgca tcggcaatca tgtgggcgat gccgcgcagt tccgccgccg cttcgcgctg
    24181 gccgtggccg gcatctatgc cccccaggaa gagcgctggg cagcgctgct ggggctggcg
    24241 ggcggcgtgc cggaagatct gctggcgccc ttcctcgtca tcgcccgcgc ggcgatggaa
    24301 gaggccccct gccccgacga cgaggccctg gcccgcgcct atggcacgcg ctcgcccggg
    24361 cgcatccgcc gcctgctcga acatctggag aaatccgggc tgatcgtggt gaagaccgat
    24421 tatggcggcc gccgggcgat cggcattccc gagctgggcg tgtctaccgc ggcggtttaa
    24481 cggcgggggg cgccgccccc ttcacccctg cggcaggatc gccgcgccca ccgcttctgc
    24541 caggcggatg ccatccaccg ccgccgaaag gatgccgccg gcatagcccg ccccctcgcc
    24601 gccgggatag agaccttgcg tgttgaggct ttgcagatcc ttgccgcggg tgatgcgcaa
    24661 ggggcaggag gtgcgcgttt ccacgcccgt catcactaca tcggggtggt catagccggc
    24721 gatcttgcgg ccgaagacgg gcagcgcctc gcggatcgct tccatcacat agggcggcag
    24781 gcaggtgcca aggtcggtca gcttcacgcc cggctggtag ctgggctcca ccgcgccgaa
    24841 atcgccgctg gaaggccgac cggccagaag atcgcccacc ttctggccgg gcgccgaata
    24901 atcgctgcca ccggccacaa aggccaggct ttcccagtgg cgctggaagg cgatgccggc
    24961 cagcggcccg ccgggataat ccttctccgg ctcgatggtg acgacaaggc cggaattggc
    25021 gttgaattcg gcgcgggaat actggctcat gccattggtc accacgcggc cttcctcgct
    25081 ggtggccgcc accacccgcc cgcccgggca catgcagaag gaatagaccg cccgcccgtt
    25141 cgacgcctga tgcgacagcg aataggccgc cggccccagc accttgttgc cggcgtgcac
    25201 gccatagcgg gccttgtcga tccagctttg cggatgttcg atgcgcacgc caatggcaaa
    25261 gggcttggct tccatgaaca cgccgcgccc gtgcagcacc tcgaacgtat cgcgcgcaga
    25321 atggcccacc gccatgatca catggcgcgc ggccagctgg ctgacagcgc cgctcgcgac
    25381 atcggtcagt tccagcccgg cgagctggcg cccctcgccc tccacgataa aatcggtgac
    25441 gcgggtgcca aagcgatatt cgccgcccag cgcctcgatc gtctcgcgga tgctcatcac
    25501 catggtgaca aggcggaagg tgccgatatg cgggtgcgct tcatagagga tgtcgtcggg
    25561 cgcgccggct ttcacgaatt cggtgagcac cttgcgggaa aggtggcgcg gatccttgat
    25621 gcgcgaatag agcttgccat cggaaaaggt gccggcgccg ccctcgccaa actgcacgtt
    25681 cgattccggc gtcagctcgc gccggcgcca caggccccag gtgtccttgg tgcgctcacg
    25741 cacagccttg ccgcgatcga tgatgatcgg cttcagcccc atctgcgcca ggatcagccc
    25801 cgccatcagc ccgcaagggc cggcgccgat caccaccggc cgctcgcccg catagccggc
    25861 aggggccatc acgggcaggc tgtaggcggt gtcgggcgcg gggcccacat gcggatcatc
    25921 ggccaggcgc gccagcacct cggcctcctc ggccacttcc acatccagca catagacgag
    25981 gcggatggcg gccttcttgc gcgcatcatt gccgcggcgc accacatgca ggcgcagcag
    26041 ccgatcgggt gtgatgccaa ggcgcgcggc agcggccgcc tccagcgctt cgggggcatg
    26101 gtcgagcggc agggcaaggt cggcaaggcg gatcatctgc gccccctatc gccgaaagcg
    26161 gccacctgtc catgggcgaa catgcggctt tggccggcct tgtcttcacc ggaagcgaat
    26221 ggtactggcc cgttccatta attttcgcaa ggcgacagcc ggttgcgcgg ccccaaggga
    26281 tgcgcgccgg gcgaaagggg cgggcgtgcc gacaggtatc aggacatgga tgatggcggg
    26341 cgcgggcgcc ctggcgctgc tggggctggg ccttggctgg cgctatgaaa cccatggccg
    26401 ctacatcgag gaaaccaacg acgcgcagct cgccgcggat tcggtggcga tcagcgccaa
    26461 gctggccggc tatgtgcgcg aagtgccggt ggtggacaat gacgcggtga gccgccgcgc
    26521 gccgctggtg aagatcgacc cggtggatta tgccaccagg ctggatgccg ccaacgccga
    26581 tatcgccgcc gccaacgccg cccaggccgc cgccagcgcc gaggccggcc aagcccgcgc
    26641 cgccgccgat gaagccgagg ccggccttgg cgaagcgcag gtggagctgg agcgcgccca
    26701 gcgcgaggaa ggccgctatc gcccgctggc cagcgccggg gcagagccgg ccgaaaagct
    26761 ggcccagctg atctcgaaca ccgcccatgc ccgcgccgaa gtggccctgc gccgcgccac
    26821 gctggcccgc atgcgccagc aggtggcgct ggcccaggcg catggccagg ccgccaccgc
    26881 ccgcgcgcaa agcgccacca ccgccgccca tgccgcccgg aacgacctcg acgccacccg
    26941 cctcaccgcg ccgatcgacg ggcgcgtggc cagccttggc gtgcgggtgg ggcagtttgt
    27001 cagccccggc cagcggctga tgacgctggt gcccgatggc agcctctatg tggtggccaa
    27061 tttcaaggaa acccagctgg ggctgatgcg cccgggccag atggccgaaa tccgcgtgga
    27121 tgcgctgccc ggcgtggtgt tccacggcga ggtggaaagc accagccccg gcaccggcgc
    27181 ccagttcagc ctcatcccgc cgcaaaacgc caccggcaat ttcaccaaga tcgtccagcg
    27241 cgtgcccgtg cgcatccgca tctatgcagg gccagtgtcg cgccgggtgc tggtgccggg
    27301 cctgtcgctg tcggtctcgg tggataccag cgcgggcaag gaagagctgg ccgccatccg
    27361 cgccgaacag gaacgccgcg gccaatgagc gaggccccct ctggcacggc gccgctcgaa
    27421 accgcggatc tggccgcctg gctggcggtg gcggcgggca cgctgggcgg tttcatggcc
    27481 tttctcgatg tgtcggtggt caattcggcg atgccggtga tccagggcga gatcggcgcc
    27541 acgcaatcgg aaggcacgtg gatttccacc gcctatctgg tggccgagat cgtcgtcatg
    27601 ccgctgacga cctggtttga ccggctgatc ggcctcaggc gcttcctgct ggccagcgcg
    27661 gtgctgttca ccctgttctc gatgctgtgc ggccttgccg gcgatttgcc gacgatgatc
    27721 tgcggccggg tgggccaggg gctggcgggc ggctcgctca tccccacggc gctgacgatc
    27781 atcgccacgc gcctgccgcc gcgtcagcag gcgatcggca tcgcgctgtt ttcctgcgcg
    27841 gcgctgctgg ggccgatcat cgggcccttg ctgggcggct ggatcaccga gacctacagc
    27901 tggcgctatg ccttctacat caacgcgccg atctgcggcg tgctggtggc gctgctgctg
    27961 ctgggcctgc cggccaaaaa gggcgactgg cacgagctgg cccgcgccga ttgggcgggc
    28021 attttcggca tggcgctggc gctgggcagc atcaccatcc tgctggaaga ggggcaccgc
    28081 gagcagtggt tcgattccgc cctcatccgc accctggcgg ccggctgcgt ggtgggctgc
    28141 gtggcggtgg gctgggggca atggcgcagc gcgcggccga tcgtcagcct ggcgctgctg
    28201 cgcaatccgg cgctgacggc caccattgta ctcagcctga tggccggcgc gctgatgttc
    28261 accagcctgt tctcggtgcc acagttcctg gccgccatcg ccggctacaa tgcctggcaa
    28321 tcgggcacgg tgatcgccat ttcgggcggc agcgccattc ttgccgccgc gctctatccg
    28381 ctggtgatcg cgcgggtgga tgtgcggctg ctggtggggg cggcctttct ggtgcaggcg
    28441 ctggcgtgct ggcaggccag ccacctcacc gccgcttccg atggcaccgc ctttgccctc
    28501 tcgcagatgc tgctgggggt gggccttacg ctggcggtgg tgccgctgca gcaggtcaat
    28561 gtctctgtcg tgcggctgga ggaggcgggc gaggcctcca gcctgttcgt cgtcgcgcgc
    28621 aatctgggcg cctcgatcgg ccttgcggcg gtggccagct ttcaggatca gcggctggaa
    28681 tttcaccact ggcgcctcca cagcgccatt gccgccagcg atccggcgat gcaggcgcag
    28741 gtggccgccg atgccgcggc ccatggcggc ggcgccgacg ggctggccgc ggcgctcacc
    28801 cttgtcgaca gccatgtgat gatggatgcg ctggttatgt ctttcaacga tgtgtttctg
    28861 gcgctggcgg tggccaccct gctggccagc ccgctggtgc tgctgctgcg cccgatagac
    28921 ccggcccgcg cgatgggggc ggcgcactga tgcgcgcgcc gatccttgtg ctgggcgcgc
    28981 tggcgctgtc ggcctgcaag ctggggcccg attatgcccg gccggccctg cccacaggcg
    29041 ctgccgccag cctgcatgcc ggcgaggcgc cgccgccgct ggcgccctgg tggccggcgc
    29101 tggccgatcc gcggctggat gcgctggagg atgaggcgct ggcccatgcc cccaccctgg
    29161 ccgaagcgcg cgcccgggtg gaagccgccc gcgccggcct gcgcgcggcc cgtgcagcgc
    29221 aggccccggc catggcggtg gacgccgcct atgcccatgc ccagctgccc ggccagttca
    29281 ccacgctcac cggcatcgac cgcgccgagg cctatgccgc cggcctcgat gccagctggg
    29341 aggtggacct gtggggcgcg cgccgccgcg cggtggaaca ggcccgcgcc ggcgccggct
    29401 tggccagcgc cagcctggcc gatgccgaag tgcggctgac agcggaactg gcccagacct
    29461 atgtggcgct ggccgcccgc caggccgccg ccaccctgct ggaacaccgc gccgggctgg
    29521 aggggcaatt gctgcacatg gcccgcctgc gcctggccgg cggcaccggc accggccagc
    29581 aggtggaaca ggccgccgcc acgctggatg ccacccgcgc cgacatcgcc ctcaacgccg
    29641 gcgaggaaag cgccctgcag gccgcgctgg cggtgctggc cgggcgggcg ccgggcgctg
    29701 cgcctgccga acggatcgcc gccgtgccgc tgccccctgc ccgggtggaa gtgggcgatg
    29761 ccggcgcgct gatcgcccgc cgccccgatg tgcgcatggc cgagcgcaat ctggccgctg
    29821 cccatgccgg catcggcatg gcgctggccc agcgctatcc ctcggtgtcg ctgctggggt
    29881 tcatcggcat tggcgggctg tcggccggcc atggcttcga cagcgcccag gccggcggcc
    29941 tgctgctgcc gcgcctcacc tgggccttcc ccgattttgg ccgggtgaat gccgccgtgc
    30001 acgccgcccg cgcggaagat gccgccgcgc tggcccatta tcaggccgcc atgctgggcg
    30061 ccctgcagga tgccgatggc gcgctggccc gctttggcgc ggcccgcagc gcctatgccg
    30121 gcgcgcttga cagcgagggc catgcgcatg aggccgcgcg ccttgaggcc ctgcgcgccg
    30181 cgcgcggcac catcgcccgc agccaggcac tggccgccga aacgcaggcg atcagcgcca
    30241 cgctggcccg gcaggccgcc gccgcccggc tgacgctcgc ctatgcgcag ctggccaagg
    30301 cgctggggct gggctggcag gggccatgag ccctgccccg ccgccgcccg cgcggggccg
    30361 cccgcaggct gcccgcgcgg cgctgatcgg ccatcgcatc atcgaggcgg cctggggcct
    30421 tttggtcgat gtcgggccag aggcgttcac catcgatgcc ctggccctgc gcgcgcgcgc
    30481 cagcaagcag acgatctacg cccgttttgc cggcaagcag cccttgctgg cagcggtgct
    30541 ggccagccgg ctgcggcgca ttctggccgc ggtggaggaa ggcgggccgg cgccctctcc
    30601 gcttgcccat ctggccgatc tggccgcccg cgcggtgctg gcgctgagcg agccggaagc
    30661 gcgcctgttc gaccggctga tcgactggat cgacaatgcc gagccggcca gcacccagcc
    30721 cacaggcacc cagcccacgc gccgcgccgt ctacgccggt ttccatgccg ccgccgaggg
    30781 gcatctcgcc catttctgcg cgcgctggca gtgccggctg gaagatctgc cggcggtggc
    30841 cgccttctgg gtggaagggc tggtgggcca tgtgcgctgc gccatcaccg atgccgatgg
    30901 ccaccgcgac tgggcccatg cctatgccgc ccatttcctg cgcgcgcagg ggtgggcagg
    30961 aaatgggcgg gcggattagg gccgcgcttc cggcaggggc gctggcgggg ccgcatggcg
    31021 cacgatatgc ggcgttacca gaatatagag ctcggtcttc gccttggtca gcttcttgtt
    31081 gtggaaggcc tggcccagca gcggcacatc gcccaggatc ggcaccttgg catcgctcga
    31141 tatcgtcgag gtctgggtga ggccgccgat gacgaagctc tcgccatcct tcaccgtcgc
    31201 cgaggtttcc gcctcgcgct ggctgatggt gggatagccc gaggaatagc ccgtcacgct
    31261 ggagacgacg cagaacacat ggctcgtcac aaagccatcg tcgctgacgc gcggggcgat
    31321 ctgcagcgtc acgcccacat tcacatattg cacctgctgc gacacgccat tgacgcccga
    31381 cagcgtgatc gaggtgagga tcggcagcgc atcgccggtg atgatcttgg cgctggcccc
    31441 gctgcgcgcg gaaatgcgcg ggcgcgagac gatgcgcccc tcgccctttt gcacctgcgc
    31501 gtaaaccgcc gcctgcaatt gcgcgctggt gagccgctgg ccgctgttgg cggtgaagcc
    31561 ggaggggata tattcgcccg tctgcaccgt ggcgaggccg atctggccgt tggcgttggt
    31621 gaaatcgatg cccaccgatc gcgccccgct ttcggtcagc tccaccagct gggtttccag
    31681 gatcacgctg tccaccggca cgtcgatgag ggcgatctcg gccttcatcc gggcgatctg
    31741 ggccgggctg ccgcgcagcc acaccgcgtt cagccgccgg tccaccgcca ccgcgctgtc
    31801 aaccgactgg ccgagcggct ggtcgtctgc cccggccatg gcgggggcgc tggcctgata
    31861 ggcgccgccg gtcaggctgg tggagccaaa gcccggctcg cgcggggtga acacatcgtt
    31921 cgatttcacc gtcagcccgt cggtcagcag gcccaccacc tcgctcacat cggcatagcg
    31981 cagcatcacc atctcatagc cttcctcgcc ggcggcgggc tggcgggcgg cgggcagcgg
    32041 gccggcgggg ccctgggcta tggcggcggg cttttca
//
LOCUS       DASRYE010000227        11425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_84455,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000227 DASRYE010000000
VERSION     DASRYE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84455"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2258
                     /locus_tag="VFF98_11710"
     CDS             <1..2258
                     /locus_tag="VFF98_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZU64343.1"
                     /translation="IRSHFASWAAAPAAAQPAAGPVAPADAGRSAVYLDPALSERLVI
                     TRRAAWRPEPDTQAQRREDLLRRIGYAIVNRRLQHIARQPNLPFREAVFGSGDLFRAG
                     RATSLVIDAVDGQWRRAMAVAAQTYRQVLDQGITRAELDEQIANIHTAAEHAAAGAAT
                     RGSGELLGAVFALLRDEAVPSDPVADAARIEGWLGAITPEAVLAALKADVVPLDNPLI
                     RFQGRKAPDGNTEGLRTAWNEAMRSPLPARTAATASGFAYADFGAPGRVVADRREPGL
                     GIREIRFANGVKLNLKHTDIEKDKVLIAVNIDGGQMLETRADPLATEMVQMLTTGGLG
                     KHSLDDLQTVLAGHEVSTSLANGPDSFVGSARTTPADLALQLRVLAALITDPGYRIEG
                     EQQYRLAVNTYFTQLNATPAAALTNSIGGILSDQDPRFSVQKVEAYRALTFARLKAAI
                     ADRLAHGALEVGLVGDVDEARAIALVGATLGALPVREADFRPYAEQRQRPFTAERTER
                     VVRHTGPANQAMLRLTWPTRDDSDPQAKQVLNMLGRVAQVELTENLRQRLGKAYAPSA
                     SSDPSRVWRGYGTFAITASVDVADLAVMRAAMLDTVAALRARPVSADIMLRARQPLVE
                     SFQNALKSNGGWLALVARAQSQPDRIARHLKAEARLAAVSAGDVQAAARRYLGAGAAV
                     EVAVLPEGAALPAPLQPAPALLAGGDHQAGKAGANPAGGPSAGASPAAPDPAPQAEPA
                     QLSPRASIASE"
     gene            2361..2615
                     /gene="purS"
                     /locus_tag="VFF98_11715"
     CDS             2361..2615
                     /gene="purS"
                     /locus_tag="VFF98_11715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZU64344.1"
                     /translation="MSGIPSSTSKVRVYVSLKNGVLDPQGRAIQHAIEGLGFSGVEDV
                     RAGRLIELEVDASVTDAALDEIANKLLANMVIENYRIERV"
     gene            2627..3298
                     /gene="purQ"
                     /locus_tag="VFF98_11720"
     CDS             2627..3298
                     /gene="purQ"
                     /locus_tag="VFF98_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZU64345.1"
                     /translation="MRSAVITFPGSNCDRDMAVAIEEVCGGEVFRVWHGDAGLPDDLD
                     FIALPGGFSYGDYLRSGAMAARSPVMRAVAEAAARGVAVLGVCNGFQVLTEAGLLPGA
                     LMRNAGLRFVCREVALSVANDSSIFTSRYAKGQRITIPVAHHDGNFFADDATLDRLEG
                     EGRVAFRYAESVNGSARNIAGVLNEKGNVLGMMPHPERHIEQAQGGIDGRALFAGLVS
                     RLVNA"
     gene            complement(3342..5600)
                     /locus_tag="VFF98_11725"
     CDS             complement(3342..5600)
                     /locus_tag="VFF98_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU64346.1"
                     /translation="MLGLQYARLGQVAAARLFVQALAALLTLRLCIGLVGPAVLSAWL
                     GALAGCAMFMTRSDQALSTLAPHALRRGDARRHVLGMVVNGLLWSVPPLLFMAHADEM
                     LRVEQWAVAALLMTGTAVIVPAVPLSTVLFCAILGSSTVLGLALHGDVGMAGLAGLFV
                     LCVVLGAVEMGRHLVESRLAMDGMAERNEVVSLLLREYEETEADWLWQIDRRGRVCHA
                     NRRFATALGRPVEAVEDALLLPLLAGAAWPAALAEAGMGLLAQRLRARESFSEVEVQV
                     RLGEEVRWWSLSGTPRHGDNGVFEGFRGVASDVTRQREHSDRIAWLARYDTLTGLPNR
                     MLVTASLDVALAEAGRQERSCGFLMIDLDRFKTVNDTQGHHMGDRLLAQVARRLEALM
                     ERQDLCGRLGGDEFAVVLEEGVDEARVAQLAEAVIHHLSEPFCIDGQLLQVGASVGSA
                     VGPRDGASVAELMRNADLALYRAKEQGRQQHHAFVPALHAQVEERHQMEKALRRALDR
                     RELSLHFQPVVRANDTEIVSFEALLRWHNPQLGQVRPDQFIALAEETRLILPIGAWVL
                     AEACRQAAGWPVPARVAVNVSGVQLLDPGFLETVVGALTASGLPAQRLDLEVTESVFL
                     SEADTARAALEAVMALGCRVALDDFGVGYSALGYLHKLRFSAIKVDRSFVVGASAGNA
                     ESVAIIRAVVALAGSLGMVTTAEGVETADQLAMVRELGCTKIQGYLFGRPMPAAEVAG
                     VLLSGVEARLAG"
     gene            complement(5778..7601)
                     /gene="glmS"
                     /locus_tag="VFF98_11730"
     CDS             complement(5778..7601)
                     /gene="glmS"
                     /locus_tag="VFF98_11730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZU64347.1"
                     /translation="MCGIIGIVGKEEVADRLVDGLRRMEYRGYDSAGVCTVHDGQLIR
                     RRAPGKLNGLVKELAVNPAPGTTGIAHTRWATHGAPTANNAHPHATGEVALVHNGIIE
                     NFRPLKEELTARGRVFESDTDTEVVAHLVSEAVEAGRSPEEAVRAALPRLRGAFALAI
                     AFRSHPDMLIGARLGSPLVLGFGEGETYLGSDALALAPLTQRITYLEEGDWVVVRANS
                     VQIFDKDNVPVARPVVASGASAAAVEKGNFRHFMQKEIYEQPTVVAQTLRSYIRQVDD
                     TVALPQIDFDLSAIKRVTIVACGTSFYAGLVAKYWFEAFARLPVDIDVASEFRYRDPV
                     LEQGGLAIFISQSGETADTLAALRHCKANGQTIAVVVNVPTSSMAREADLLLPTHAGP
                     EIGVASTKAFTCQLAVLAALAAHLAVKRGRMSREEERSVVQQLIETPAALNAALAHDD
                     EIAGMAHLIAPARDVLYLGRGQDYPLALEGALKLKEISYIHAEGYASGEMKHGPIALI
                     DENVPVIVLAPSGPLFEKTVSNMQEVRARGGKIVLISDAEGLAEAGEGCMATIEMPKV
                     HPLIAPLVYAVPVQLLAYHVACVKGTDVDQPRNLAKSVTVE"
     gene            complement(7726..9096)
                     /gene="glmU"
                     /locus_tag="VFF98_11735"
     CDS             complement(7726..9096)
                     /gene="glmU"
                     /locus_tag="VFF98_11735"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZU64348.1"
                     /translation="MTAEIASLAIVVLAAGKGTRMKSATHKVLHPIAGRPMLEHLLAS
                     AAELAPGAQVVVAGHGREQLEKALAGRAAIAVQDPQLGTGHAVQMAQGALAGLDPATP
                     VLILYGDVPFVAPDTMRAMVARLLAEDAPAVVVLGFTPQDTLQYGRVIAEGGRVLKMV
                     EHKDASEAERACGLCNSGLMAARAGDLFALLARVGNDNAQGEYYLTDIVNIANADGRA
                     CAVVVTADEHEVMGINSRGELAIAEARWQQRRRAAAMADGATLVAPETVWFSWDTVLG
                     RDVLVEPNVWFGPGAVVADGVTIHAFCHIEGARIAPGAEVGPYARLRPGAVIGGKAKV
                     GNFVEIKNATMGEGAKANHLSYIGDAEVGAHANIGAGTITCNYDGYFKHKTVIGERAF
                     IGSNSALIAPVKIGADAIVAAGSAVSRDVAAGELRLVRAEQLVKPGWADQFHDAMRRK
                     KAEKKG"
     gene            9157..9852
                     /locus_tag="VFF98_11740"
     CDS             9157..9852
                     /locus_tag="VFF98_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZU64349.1"
                     /translation="MAKIPFDVVGFDLDGTLLDTSGDLGVALNHTLGLAGRPAVAMEK
                     VRDLVGGGTRMMLERALAITGGPLPADELRALSATLVAHYEAHIADHSRLFPGGEAML
                     DALAATGAKLAVVTNKLEHLAVKLLAELGLAERFYTIIGGDTLGPGRAKPRPDLLQEM
                     VARAGLPARPRAAYVGDTSFDTGAAQAAGLPCVVVRFGFNDRPAEELGGSAIIDHYDE
                     LVPALARLGADAD"
     gene            10030..10230
                     /locus_tag="VFF98_11745"
     CDS             10030..10230
                     /locus_tag="VFF98_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64350.1"
                     /translation="MFLDLRQEGIPPEPWQPPRRRRSLSHREKQAVAGIAGFNLLLLL
                     LAPVGGVGLVEALAYLLHPLLR"
     gene            complement(10248..10577)
                     /locus_tag="VFF98_11750"
     CDS             complement(10248..10577)
                     /locus_tag="VFF98_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HZU64351.1"
                     /translation="MIAFPRGGAPQVGFDRLELTRILDLYGRMVAAGLWRDYAMNFDR
                     EAASFSAFCRTAERPQARIEKRPALRSKQGMWTLFGEGGQVLKRGRDLAGVLFPIERR
                     LLKAVDG"
     gene            complement(10692..11381)
                     /gene="epsC"
                     /locus_tag="VFF98_11755"
     CDS             complement(10692..11381)
                     /gene="epsC"
                     /locus_tag="VFF98_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZU64352.1"
                     /translation="MFSRLIAYLDSVVARDPAPRSRWEVLLYPGFWAVGFHRVAHALF
                     GARLFFLARAVNHLARFLTAIDIHPGATVGRHLFIDHGFTVIGETAHIGDNVTIYQCV
                     TLGGTNPANGIGGKRHPTLSDDCIIGSGAQILGPITVGRRARVGANAVVTEDVPEGAT
                     MVGVKARSTLVAAESWAKDFVPYGTPCREPCADAEPASQRIATLEAQVAELSAKLEAM
                     LEPAPRDGTKG"
BASE COUNT         1869 a         4125 c         3710 g         1721 t
ORIGIN      
        1 ccatccgcag ccatttcgcc agctgggcag ccgcgccggc cgccgcccag cccgccgccg
       61 gccccgtggc cccggccgat gccgggcgca gcgcggtcta tctcgatccg gcgctgtcgg
      121 agcggctggt gatcactcgc cgcgccgcct ggcggccaga gcccgacacc caggcccagc
      181 ggcgcgagga tctgctccgc cgcatcggct atgccatcgt caaccgccgg ctgcagcata
      241 tcgcccgcca gcccaacctg cccttccgcg aggcggtgtt cggcagcggc gacctgttcc
      301 gcgccggccg cgccaccagc ctggtgatcg atgcggtgga tggccagtgg cgccgggcga
      361 tggcggtggc cgcgcaaacc taccggcagg tgctggacca gggcatcacc cgcgccgagc
      421 tggacgagca gatcgccaat atccacaccg ctgccgaaca tgccgccgcc ggcgctgcca
      481 cgcgcggctc tggcgagctg ctgggcgcgg tcttcgcgct gctgcgcgac gaggccgtgc
      541 ccagcgaccc ggtggccgac gccgcgcgca tcgagggctg gctgggcgcg atcacgcccg
      601 aggcggtgct ggccgcgctg aaggccgatg tcgtgccgct ggacaatccg ctgatccgct
      661 ttcagggcag gaaagcgccc gatggcaaca cagaaggcct gcgcaccgcc tggaacgagg
      721 ccatgcgcag cccgctgccc gcccgcactg ctgccaccgc cagcggcttt gcctatgccg
      781 atttcggcgc ccccggccgc gtggtggcag accggcgcga gcctggcctt ggcatccgcg
      841 agatccgctt tgccaatggc gtgaagctga acctcaagca caccgacatc gagaaggaca
      901 aggtgctgat cgccgtcaac atcgatggcg ggcagatgct ggagacgcgc gccgatccgc
      961 tggccaccga aatggtgcag atgctgacga ccggcggcct tggcaagcac agcctcgatg
     1021 atctgcaaac cgtgctggcc ggccatgagg tgagcaccag cctggccaac gggcccgaca
     1081 gtttcgtggg cagcgcgcgc accaccccgg ccgatctggc gctgcagctg cgggtgctgg
     1141 ccgcgctgat caccgatccg ggctaccgca tcgagggcga gcagcaatat cggctggcgg
     1201 tgaacaccta tttcacccag ctcaacgcaa cgcccgccgc tgccctcacc aacagcatcg
     1261 gcggcatcct ctcggatcag gatccgcgct tcagcgtgca gaaggtggag gcctatcgcg
     1321 cgctcacctt cgcccggctg aaagcggcga ttgccgaccg gctggcccat ggcgcgctgg
     1381 aagtggggct ggtgggcgat gtggacgagg cccgggcgat cgcgctggtg ggggcaacgc
     1441 tgggcgccct gccggtgcgc gaggcggatt tccgccccta tgccgagcag cgccagcgcc
     1501 cctttaccgc cgagcggaca gagcgcgtgg tgcgccacac ggggcctgcc aaccaggcca
     1561 tgctgcgcct cacctggccc acgcgcgatg acagcgatcc gcaggccaag caggtgctca
     1621 acatgctggg ccgggtggcg caggtggagc tgaccgagaa tttgcgccag cgcctgggca
     1681 aggcctatgc gccatcggcc agcagcgatc cctcgcgcgt gtggcgcggc tatggcacct
     1741 ttgccatcac cgcttctgtc gatgtggccg atctggcggt gatgcgggcg gccatgctcg
     1801 acacggtggc ggccctgcgc gcgcggccgg tgagcgccga catcatgctg cgcgcccgcc
     1861 agccgctggt ggaaagcttc cagaacgcgc tgaaaagcaa tggcggctgg ctggcgctgg
     1921 tggcccgcgc gcaaagccag ccggatcgca tcgcccgcca tctgaaggcg gaagcacggc
     1981 tggccgcggt cagcgccggc gatgtgcagg cggcggcgcg gcgctatctg ggcgcgggcg
     2041 cggcggtgga agtggcggtg ctgcccgaag gcgccgccct gcccgccccg ctgcaaccgg
     2101 cgccggccct gctggcgggc ggcgatcatc aggccggcaa ggccggcgcc aatccggccg
     2161 gcgggcccag cgccggcgcc agcccggccg cgcccgatcc cgccccccag gcagaacccg
     2221 cccagctctc gccgcgcgct tccatcgcca gcgaataggc cctgccgccc gcccctttgc
     2281 gcacggggct tcccttttgg ccgcaaggcc gccataggca gcgcttgatt ccgtttatgc
     2341 agccagaaag gcccaccgcg atgtccggca tcccttccag caccagcaag gtgcgcgtgt
     2401 atgtcagcct gaagaatggc gtgctcgatc cgcagggccg cgccatccag catgcgatcg
     2461 aggggctggg cttctcgggc gtggaagatg tgcgcgccgg ccggctgatc gagctggaag
     2521 tggacgccag cgtgaccgat gcggcgctgg atgagatcgc caacaagctg ctcgccaata
     2581 tggtgatcga aaactaccgc atcgagaggg tgtaagcatc atggcgctgc gttcggcggt
     2641 tatcaccttc cccggctcca actgcgaccg cgacatggcg gtggcgatcg aagaggtgtg
     2701 cggcggcgag gtgttccggg tgtggcatgg cgatgccggc ctgcccgatg accttgattt
     2761 catcgccctg cccggcggct tttcctatgg cgactatctg cgctcgggcg cgatggcggc
     2821 gcgcagcccg gtgatgcggg cggtggccga agcggccgcg cgcggcgttg ccgtgctggg
     2881 cgtgtgcaac ggcttccagg tgctgaccga ggccggcctg ctgcccggcg cgctgatgcg
     2941 caatgccggc ctgcgcttcg tctgccgcga agtggcgctg agcgtggcga acgacagctc
     3001 gatcttcacc agccgctatg ccaagggcca gcgcatcacc attccggtgg cccatcacga
     3061 tggcaatttc tttgccgatg acgcgacgct ggaccggctg gaaggcgaag gccgcgtggc
     3121 cttccgctat gccgaaagcg tgaacggttc ggcccggaat atcgccggcg tgctgaacga
     3181 gaagggcaat gtgctgggca tgatgcccca ccccgagcgc catatcgagc aggcccaggg
     3241 cggcatcgat ggccgcgcgc tgtttgccgg gctggtcagc cggctggtga acgcctgaga
     3301 tcgcaccagg cgggggcggc cagcggccct cccgcctgcc atcagccagc caggcgggct
     3361 tccacgccgc tcagcagcac gccggccacc tctgccgccg gcatcggccg gccaaacaga
     3421 tagccctgga tcttggtgca gcccagttcg cgcaccatcg ccagctgatc ggcggtttcc
     3481 acgccctcgg ccgttgtcac catgcccagc gagccagcca gcgccaccac cgcgcggatg
     3541 atcgccacgc tttcggcatt gcccgcagaa gcgcccacca caaagctgcg atccaccttg
     3601 atggcggaaa agcgcaattt gtgcagatag cccagcgccg aatagcccac gccaaaatca
     3661 tccagcgcca cccggcagcc cagcgccatc accgcttcca gcgccgcgcg ggcggtatcg
     3721 gcctcggaga ggaagacgct ttcggtcact tccagatcca gccgctgggc cggcagaccg
     3781 ctggccgtca gcgcccccac caccgtttcg agaaagcccg gatcaagcaa ctgcacgccc
     3841 gagacattca ccgccacgcg cgccggcacc ggccagccgg ccgcctgccg gcaggcctct
     3901 gccagcaccc aggcgccgat cggcaggatc agccgcgtct cttcggcaag ggcaatgaac
     3961 tgatcgggcc gcacctggcc cagctgcgga ttgtgccagc gcagcagcgc ctcgaagctg
     4021 acgatctcgg tatcgttggc gcgcaccacc ggctggaaat gcaggctcag ctcgcgccgg
     4081 tccagcgcgc ggcgcagggc cttttccatc tggtggcgct cttccacctg ggcgtggagc
     4141 gcgggcacga aggcatggtg ctgctggcgc ccctgctcct tggcgcgata gagcgccaga
     4201 tcggcgttgc gcatcagctc ggccacgctg gcgccatcac gcgggcccac cgcggagccc
     4261 acgctggcgc ccacctgcag cagctgcccg tcgatgcaga agggctccga caggtggtgg
     4321 atcaccgctt cggccagctg cgccacgcgc gcctcgtcca cgccttcttc cagcaccacg
     4381 gcaaattcat cgccgcccag ccggccgcac agatcctgcc gctccatcag tgcttccagc
     4441 cgccgcgcca cctgggccag cagccggtcg cccatatggt ggccttgcgt gtcgttcacc
     4501 gtcttgaagc ggtcgaggtc gatcatcagg aagccgcaac tgcgctcctg ccggccggct
     4561 tcggccagcg ccacatccag gctggcggtt accagcatgc ggttgggcag gccggtcagc
     4621 gtgtcatagc gggcaagcca ggcgatgcgg tcggaatgtt cgcgctggcg ggtgacatcg
     4681 gaagcaacgc cgcggaagcc ctcgaacacg ccattatcac catggcgcgg cgtgcccgaa
     4741 agcgaccacc agcgcacttc ctcgcccagc cgcacctgca cttccacttc ggaaaagctt
     4801 tcccgcgcgc gcaggcgctg ggccagcagg cccatgcccg cctcggccag cgccgccggc
     4861 caggcagcgc ccgccagcag cggcagcagc aatgcgtctt ccaccgcctc caccgggcgg
     4921 cccagcgcgg tggcaaagcg ccggttggca tggcacaccc gcccgcgccg gtcgatctgc
     4981 cacagccagt cggcctcggt ctcctcatat tcgcgcagca gcaggctgac gacctcgttg
     5041 cgctcggcca tgccatccat cgccaggcgc gattcgacca gatggcggcc catttccacc
     5101 gcccccagca ccacgcacag cacgaaaagc ccggcaagcc cggccatccc cacatcgcca
     5161 tgcagcgcca gccccagcac cgtggaactg cccagaatgg cgcagaacag caccgtggaa
     5221 agcggcaccg ccggcacgat caccgccgtg ccggtcatca gcagggcggc caccgcccat
     5281 tgttccaccc gcagcatctc gtcggcatgg gccatgaaca gcagcggcgg caccgaccac
     5341 aacagcccgt tcaccaccat gcccagcaca tggcgccgcg catcgccccg gcgcagcgca
     5401 tggggcgcca gggtagacag cgcctgatcc gatcgggtca tgaacatggc gcagccggcc
     5461 agcgccccca gccaggccga cagcaccgcc ggccccacca ggccaatgca cagccgcaag
     5521 gtgagcagcg cggccagcgc ctgcacaaac agccgcgcgg ccgccacctg ccccagccgg
     5581 gcatattgca gccccagcat gcgcgcagca tccacccgcc cctgccggga caagcccagc
     5641 agcgcccagg ccgtgagggg cctggatacg gcctcggaag aagaagcgga cgtcatcgcc
     5701 tgacagcgat gcacgggatc cgctaatttt aggtaaattt acttaggcat ttgcccgtga
     5761 tttgtccaaa tcggtgctca ttccaccgtc accgatttgg cgagattgcg cggctgatcg
     5821 acatccgtgc ccttcacgca ggccacgtga taggccagca gctgcaccgg caccgcatag
     5881 accagcggcg cgatcagcgg gtgcaccttg ggcatctcga tcgtggccat gcagccctcg
     5941 ccggcctcgg ccagcccctc ggcatcggag atcagcacga tcttgccgcc gcgcgcgcgc
     6001 acttcctgca tgttgctcac cgtcttctcg aacagcgggc cggaaggcgc cagcacgatc
     6061 accggcacgt tctcgtcgat cagcgcgatg gggccatgct tcatctcgcc gctggcatag
     6121 ccttcggcgt ggatatagct gatttccttg agcttcagcg cgccttccag ggccagcgga
     6181 taatcctgcc cgcggcccag atagagcacg tcgcgcgccg gcgcgatgag gtgggccatg
     6241 ccggcaatct cgtcatcatg cgccagcgcc gcattcagcg cggccggcgt ctcgatcagc
     6301 tgctgcacca cagagcgctc ttcctcgcgg ctcatgcggc cgcgcttcac cgccagatgc
     6361 gcggccagcg ccgccagcac cgccagctgg caagtgaagg ccttggtgga agccacgccg
     6421 atttccggcc cggcatgggt gggcagcagc agatcggcct cgcgcgccat gctgctggtg
     6481 ggcacgttca ccaccacggc gatcgtctgc ccgttggcct tgcagtggcg cagggccgcc
     6541 agcgtgtcgg cggtctcgcc gctctggctg atgaagatgg ccagcccccc ctgctccagc
     6601 accggatcgc ggtagcggaa ttcggaagcc acatcgatat ccaccggcag gcgggcgaaa
     6661 gcctcgaacc agtattttgc cacaaggccc gcatagaagg aagtgccgca agccacgatc
     6721 gtcacgcgct tgatggcgga aaggtcgaaa tcgatctgcg gcagggccac ggtgtcgtcc
     6781 acctggcgga tgtagctgcg cagggtctgc gccaccacgg tcggctgctc gtagatctcc
     6841 ttctgcatga agtggcggaa attgcccttt tccactgccg ccgcagacgc gccagaagcc
     6901 accaccggcc gcgccaccgg cacattgtcc ttgtcgaaga tctgcacgct gttggcgcgc
     6961 accaccaccc agtcgccctc ttccagatag gtgatgcgct gggtcagcgg cgccagcgcc
     7021 agcgcgtcgg agccgagata ggtctcgccc tcgccaaagc ccagcaccag cggcgagccc
     7081 agccgcgcgc cgatcagcat gtcggggtgg ctgcgaaagg caatggccag cgcaaaggcc
     7141 ccgcgcaggc gcggcaacgc cgcccgcacc gcttcctccg gcgagcgccc cgcctccacc
     7201 gcttccgaca ccagatgcgc caccacctcg gtatcggtgt cgctttcaaa cacgcggccg
     7261 cgcgcggtca gctcttcctt cagcgggcgg aaattctcga tgatgccatt gtgcaccagc
     7321 gccacctcgc cagtggcatg ggggtgggcg ttgttggcgg tgggcgcgcc atgagtggcc
     7381 cagcgggtgt gggcaatgcc ggtggtgccg ggcgcggggt tcaccgccag ctccttcacc
     7441 agcccgttaa gcttgcccgg cgcgcgccgg cggatcagct ggccatcatg caccgtgcac
     7501 acgccggccg aatcatagcc gcgatattcc atccgcctga ggccatcgac cagacgatcc
     7561 gctacctctt ccttgccgac gataccgatg atgccgcaca tagatctgct gccctacccc
     7621 tgaaacgttt gcgccgcagg ccgcgccctt caaacagcgc gcccgcctgt gccccatgca
     7681 cattaggcga tgaatgcgat attgccagcc gcgagcggcg gccgatcagc ctttcttttc
     7741 ggcctttttc ctgcgcatgg catcgtggaa ctggtctgcc cagcccggtt tcaccaattg
     7801 ctcggcgcgc accagccgca gctcgcccgc cgccacatcg cggctgacgg cactgcccgc
     7861 cgccacgatc gcatccgcgc cgatcttcac aggggcgatc agcgccgaat tggagccgat
     7921 gaaagcccgc tcgccgatca ccgtcttgtg cttaaaatag ccgtcgtagt tgcaggtgat
     7981 ggtgccggcg ccaatattgg catgggcccc cacttccgca tcgccgatat aggagaggtg
     8041 gttggccttg gcgccctcgc ccatcgtggc gttcttgatc tcgacgaaat tgcccacctt
     8101 ggccttgccg ccaatcaccg cgccggggcg caggcgggca taggggccca cttcggcgcc
     8161 cggcgcaatg cgcgcgccct cgatatggca gaaagcgtgg atcgtcaccc cgtcggccac
     8221 cacggcgccc gggccaaacc acacattggg ctccaccagc acatcgcggc ccagcaccgt
     8281 atcccagctg aaccacaccg tttcgggcgc caccagcgtg gcgccatcgg ccatggcggc
     8341 ggcgcggcgg cgctgctgcc agcgcgcctc ggcaatcgcc agctcgccgc ggctgttgat
     8401 gcccatcacc tcatgctcgt cggcggtgac gaccaccgcg caggcccgcc catcggcatt
     8461 ggcaatgttg acgatatcgg tgaggtaata ttcgccctgg gcgttatcat tgcccacccg
     8521 cgccagcagg gcaaacagat cgcccgcgcg cgcggccatc aggcccgaat tgcacaggcc
     8581 gcaggcgcgc tccgcctcgc tggcatcctt gtgctccacc atcttcagca cccggccgcc
     8641 ctcggcgatc acccggccat attgcagcgt gtcctgcggg gtaaagccca gcaccactac
     8701 cgccggcgcg tcctcggcca gcaggcgggc caccatcgcg cgcatcgtat cgggcgccac
     8761 gaagggcaca tcgccataga gaatcagcac cggcgtcgcc ggatccagcc ccgccagcgc
     8821 cccttgcgcc atctgcacgg catggccggt gcccagctgc ggatcctgca cggcgatggc
     8881 cgcgcgccct gccagcgcct tttccagctg ctcgcgcccg tggccggcca ccaccacctg
     8941 ggcgccgggc gccagttcgg cagcgcttgc cagcagatgc tccagcatcg gccgcccggc
     9001 gatggggtgc agcaccttgt gggtggcact tttcatgcgg gtgcccttgc cggcggccag
     9061 gacgacgatg gcgagcgatg cgatctcagc ggtcatgcgc ttcccttgcc agcaaattct
     9121 tgcaagttcc atacgggcag ggcatcgcag cggccgatgg caaaaattcc tttcgatgtg
     9181 gtcggcttcg acctcgatgg caccctgctc gacacctcgg gcgatctggg cgtggcgctc
     9241 aaccacacgc tggggctggc cgggcggccg gccgtggcga tggagaaggt gcgcgatctg
     9301 gtgggcggcg gcacccgcat gatgctggaa cgcgccctgg ccatcaccgg cggcccgctg
     9361 ccggccgacg agctgcgcgc gctgagcgcc acgctggtgg cccattacga ggcgcatatc
     9421 gccgatcaca gccgcctgtt ccccggcggc gaggcgatgc tggatgcgct ggccgccacc
     9481 ggcgcgaagc tggcggtggt gaccaacaag ctggagcatc tggcggtgaa gctgctggcc
     9541 gagctgggcc tggcggagcg cttctacacc atcatcggcg gcgacacgct gggccccggc
     9601 cgtgccaagc cgcggcccga tctgctgcag gaaatggtgg cccgcgcggg cctgcctgcg
     9661 cgcccgcgcg ccgcctatgt gggcgacacc agcttcgata cgggcgcggc gcaagcggcc
     9721 ggcctgccct gcgtggtggt gcgcttcggc ttcaacgacc ggcccgccga ggaactgggc
     9781 ggcagcgcca tcatcgatca ttacgacgag ctggtgccgg cgctggcaag gctgggcgca
     9841 gacgccgact gaacacgggc ggctgaacac gggcgggcct tgcgtccaaa atttggccaa
     9901 actgcctcaa acccgtacaa aactgcgcaa aaggcggttg cacccggcct tggccagagg
     9961 catccttctg agtctgcaaa aacagaatcg ccaaaaaaag cagacttaat gttgggaggc
    10021 ctgggagcca tgtttcttga tctgcgtcag gaaggtattc cgccggaacc gtggcagccg
    10081 ccgcgtcggc gtcgttcgct cagccatcgc gagaaacagg cggtggccgg cattgcgggg
    10141 ttcaacctgc tgttgttgct gctggcgccg gtgggcggcg tggggctggt ggaagcgctg
    10201 gcctatctac tgcacccgct gttgcgataa cgcgcaagaa aagcgcctca gccatccacc
    10261 gccttgagca ggcggcgctc gatggggaac agcacgccgg ccagatcgcg cccgcgcttc
    10321 agcacctgcc cgccctcgcc aaacagggtc cacatgccct gtttgctgcg cagggccggg
    10381 cgcttctcga tgcgcgcctg gggccgttcg gccgtgcggc aaaaggcgct gaagctggcc
    10441 gcttccctgt cgaaattcat cgcataatcg cgccacaggc cggccgccac catgcggcca
    10501 taaagatcga ggatgcgcgt cagctccagc cggtcgaacc ccacctgggg ggcgccgccg
    10561 cgcggaaagg cgatgatatt ggcagcgcct gagccctgac ctcctgcgcc ctggcctcct
    10621 gcgccatgcc cgggcgtggc ggaagcggat gcccccttgc cctcgcccgg cttgcgctcc
    10681 atcggcaggg cctagccctt cgtcccgtcg cgcggggcgg gctccagcat ggcttccagc
    10741 ttggcgctca gctctgccac ctgcgcttcc agcgtggcga tgcgctggct ggccggctct
    10801 gcatcggcgc aaggctcgcg gcagggggtg ccatagggca cgaaatcctt cgcccagctt
    10861 tccgccgcca ccagcgtgga gcgcgccttc acgcccacca tcgtcgcgcc ttcgggcacg
    10921 tcttccgtca ccaccgcatt ggcgcccacg cgcgcgcgcc ggcccacggt gatgggcccc
    10981 aggatctgcg cgcccgagcc gatgatgcaa tcatccgaca gagtggggtg gcgcttgccg
    11041 ccaatgccat tggccggatt ggtgccgccc agcgtaacgc actgatagat ggtgacgtta
    11101 tcgccgatat gggccgtctc gccgatcacg gtgaagccat ggtcgataaa caggtggcgg
    11161 cccacggtgg cgccggggtg aatatcgatg gcggtgagaa agcgcgccag atggttgacc
    11221 gcgcgcgcca ggaaaaacag ccgcgcacca aacagggcat gggccacgcg atgaaaaccc
    11281 accgcccaga agcccggata cagcagcact tcccaacgcg aacgcggcgc cggatcgcgc
    11341 gcgacaacgg aatccagata ggcaatcaat cggctgaaca tcgcctttta tcccatgaga
    11401 gcccgcttat ggcaagcgcg gctgg
//
LOCUS       DASRYE010000228        32226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_119609,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000228 DASRYE010000000
VERSION     DASRYE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 32226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32226
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119609"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFF98_11760"
     CDS             complement(<1..1169)
                     /locus_tag="VFF98_11760"
                     /inference="COORDINATES: protein motif:HMM:NF007640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaI family glycosyltransferase"
                     /protein_id="HZU64353.1"
                     /translation="MSSAMRLAILGINYAPEEIGIARYTTDMAVELAARGHVVEVVAG
                     KPYYPAWQVPQAWRGGWRRSGEAGVGIMRCPLYVPREPNGLRRIVHLISFALSALGPA
                     LMLARRPREERPQVVLCVAPALLSVPVAWLMARLAGARLWVHVQDFEVEAAFATGLVG
                     GRGWLKWLARRMEQRILRLADRASSISPQMCVKLREKGFAAEEVVEIRNWANARGSYG
                     PPEESGYRAQWGLGGRKVCLYSGNIANKQGIEILVETARLLAGRTDIAFVICGEGPNR
                     ARLMELAGGLDNIQFHDLQPLERLNDLLSLASVHLLPQIPGAADLVLPSKLTNMLASG
                     RPVVATAEPGTGLYEEVIGCGLAVPPGDAQALAQAIAQLVDEPAIAAEMGRRAVDR"
     gene            complement(1293..2228)
                     /locus_tag="VFF98_11765"
     CDS             complement(1293..2228)
                     /locus_tag="VFF98_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HZU64354.1"
                     /translation="MTFSLAGKRVFVAGHRGMVGSAVVRRLGRENCEVLTAGREAMNL
                     IEQAEVRAWMARERPEVVVVAAARVGGILANSTYPADFLYDNLMIEANLIEAARVAGA
                     QKLLFLGSSCIYPKFAEQPIREESLLTGPLEPTNEWYAIAKIAGIKLCQAYRQQYGVD
                     FISAMPTNLYGPGDNFDLNSSHVLPALIRKAHEARLAGHDSFEIWGTGTPRREFMHAD
                     DLADACIFLLQHYSDPLPINIGYGEDVTIGDLADLVARTVGFTGEVTRDASKPDGTPR
                     KLMDSSRLHAMGWKPAIPLEAGIADAYEHFLKAQK"
     gene            complement(2339..3415)
                     /gene="gmd"
                     /locus_tag="VFF98_11770"
     CDS             complement(2339..3415)
                     /gene="gmd"
                     /locus_tag="VFF98_11770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZU64355.1"
                     /translation="MVETKHKKVALITGITGQDGAYLAQLLLDKGYEVHGIKRRSSSF
                     NTGRIEEIYQDPHVEGARFHLHYGDMTDSTNIIRIIQQVQPDEIYNLAAQSHVLVSFE
                     TPEYTANADAVGTLRILEAIRILGLEKKTRFYQASTSELYGLVQEVPQRETTPFYPRS
                     PYGVAKLYGYWIVVNYREAYGMFACNGILFNHESPLRGETFVTRKITRAAAAISLGRQ
                     EKLYLGNLDAQRDWGHAREYVRGMWMMLQQDEPDDFVLATGETTRVREFVRWAFEDVG
                     IDLEFRGEGIDEKGYCQKTGKCLIEIDPRYFRPTEVELLIGDPTKAREKLGWSHQTSP
                     RDLAREMVQADLMVMRDAPIAKGS"
     gene            3672..4100
                     /locus_tag="VFF98_11775"
     CDS             3672..4100
                     /locus_tag="VFF98_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZU64356.1"
                     /translation="MRRLTGVTGGMAGLLVAGLAMAGSAQAQDWRGYDGPPPYARGWQ
                     EGEGLRMIDLVCSGRRAHMLEARLGHEVEEGDIDPDTAGRMHDAIDGLEGRQRRECAE
                     GDVRSVREIGWRYDRIGQWMEAAAHGHEGWRDWRPGWGAR"
     gene            complement(4097..6253)
                     /locus_tag="VFF98_11780"
     CDS             complement(4097..6253)
                     /locus_tag="VFF98_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64357.1"
                     /translation="MRLGQILHLGLACMVAAGSAGLPAPGAIAHAAPAPHAPQRPAHD
                     DDDDDGDDGPVQTGAIVVTARQLDAARASINPALGASAYLLTNDAVEKRPSGETANLV
                     NVLLQMPGVSQGGDGAIRVRGQDHVQYRLNNIILPDGLADPADTLRARFADRVELVTG
                     ALPAQYGLQTGGIVNLTTKSGVYQHDGEVELYGGTQGRFEPAFELAGAEAGTSYYVSG
                     AWLRSDAGLPAPDASATPLHDHTDQGDALAYFERLLDARSRLALILGVSDDGLQTPRS
                     RSGPASTITGTTTGTITGTAAGGAQQRASQYGLLSYLRSAGALNLQLAAFLSHGVQRL
                     EGDVPGDLALAGIARQDRADLLSSGLQAEAVYAPGRAHHLRAGLLASWRRTRSRAADT
                     VLLADSPLTLRGQASARRQETGLYLQDEWAAAPGLTFNYGLRFDRVSGSGGGSAPGPR
                     ASLVWSPAKATTFHLGYARYFVPAPQEREAIAPAAYAGSSAALPTATDTPARAERDDY
                     IDIGLTQKLGGLALGIDAFWRGSRNLLDQTWSADGLVAHAFNYARGTGKGVELSANWS
                     EGPLSAWGNLAVSRLQGWRIVSGQAPFSAAALAGAASQAVATNSDQRFTGSAGLAWRT
                     GPWRLSGDATFGSGLPRSATALAPNGATMPAWVALNLAAVRRFAGWGRRPLDIRLDVL
                     NALDGARRWQDGTGLAGGQPQWAARRGVFIGLEQAL"
     gene            6349..7026
                     /locus_tag="VFF98_11785"
     CDS             6349..7026
                     /locus_tag="VFF98_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU64358.1"
                     /translation="MRVLVVEDEGRIAELVSAALRAAGFAVDVCASAGDGAAAQELTA
                     YDALVLDLGLPDGDGLAVLRAARRGGGSVPVLILTARDAVEDRVAGLDAGADDYLTKP
                     FATAELISRIKALLRRPGLALGATLAAGGLLFDTLGREAQVAGRPLALARQELAILEH
                     LMRRLGRVVPRDVLEEKLYGEGGEIASNAIPVHIHHLRRKLADAGAGCAIQTVRGIGY
                     FLAEERA"
     gene            7023..8063
                     /locus_tag="VFF98_11790"
     CDS             7023..8063
                     /locus_tag="VFF98_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU64359.1"
                     /translation="MTGRWRASLEGRLILRLVAACLAASVAGFAGLVIKSWRIARGLS
                     DEGAADIFVGEFLKEAGWAFPLFALLIITLVVATVRSALRPLAAAAQQAAAIAPARAD
                     RRLPAAGLPGEILPFVGAVNAALERLEQGFEAQRRFTADAAHELRTPLAILIAGLEAL
                     PEGAPVAALRADAARMGRLVDQLLRVARLDALPAPVLVRQDLAAGAAGLVAAMAPWAH
                     GQGRSLAFEAPAGPAWVQADADSLASALRNLIENAVFHAPRGGEVLVRVEGACVRVID
                     RGAGVAAGASERLFDRFWRAADTGRHGAGLGLAIVAGVAQAHGGRACHEETPGGGATF
                     VLQLVPAGPAEG"
     gene            complement(8098..10656)
                     /locus_tag="VFF98_11795"
     CDS             complement(8098..10656)
                     /locus_tag="VFF98_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64360.1"
                     /translation="MRYSSASLVALACALLPHAGLAADADEEATANKADAIIVSARLD
                     EAARSEQKSAPNLINIQAAESIAKYPDVNAAEAISRIPGVSLSIDTSEGRFVNIRGID
                     GNFDGATMGGVVLLNTQPGGTYFNAAGRAVEFDTVPIGAVDRIAVIKTGLPDHDAEGI
                     GGSIELTPRTALHANRLFADVTLGGGIETFAGKGLYRDEVVLGGPIGGAGSPVSFVVS
                     QFLYNDHRSFHDIEESYVDDTSKAPDKAYGALEMRKYDYYRQRFGYSGELDYAPDATQ
                     RYYLRASLAGYNEHVYRNRLEIDFNGNTATNPANANGLVADTVNPVKTLRDEDETHRN
                     LVLQLGGDNQLGKLHVDYWGAYSRATYDKHFDYNSTFENANNYTLTYDNTTNPNYPTY
                     AVTSGTGFTDPANYALTKIGNATEHDRDEEWSYALSGALPVGLAANDEVKIGVKQRWR
                     HKVANSYAGTLDITNGASLSQYAGSSPITDFYRNGYNIGPMIAGQAMRDIFNASGTAL
                     TQVAGNYFDDNEDITAAFVQYKGDFGKLGVLAGLRFEHTSATYRGIGNTAVVSGSGSS
                     TTVTTVTGPLSTRHNYDNVFPTLQLHYQADAHLVLRATYSTGLARPGFYQTQQSTSID
                     TGAQTVSTGNPNLLPTYSHNFDLSAEYYLPGAGIVSAGLFDKELLDFIVTRGVPVGNY
                     AGSGYTYVVNTYENAHHAHARGIEGNFVDHFRGLPGWLGGFGIDANITYVASSVALRN
                     GEGNVAMPGTIPWSWNAAVFYERGKLKARLASQFEGTVLFGVGSTRATDVFQDSRYTL
                     DFSGSYDISRRVQLYLNMKNLTNAPLRFYEGSPNRPIQREFYDLTLETGVKLKF"
     gene            10952..11629
                     /locus_tag="VFF98_11800"
     CDS             10952..11629
                     /locus_tag="VFF98_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU64361.1"
                     /translation="MKLLLLEDDAETIAHVTRILRADGHVVDVARTGPEAIFLATTAA
                     HAVLVLDRMVPGLDGLATLKAIRAAGVQTPALFLTAMGDVTDRVEGLNAGADDYLGKP
                     FAASELLARIAALGRRTPQIDVVTRIVIADLEIDLLKRQVARAGRRIELQHQEYRLLE
                     FLARHAGQVVTRTMLLESIWDFHFEPGTNLIESHISRLRGKVDRGFDVELIRTVRGAG
                     YRLDAPC"
     gene            11616..13031
                     /locus_tag="VFF98_11805"
     CDS             11616..13031
                     /locus_tag="VFF98_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64362.1"
                     /translation="MRPARSGRLPTTGAAPIRPLASGAFRFALVLAGVFALTSAVLLF
                     LVAHLVDHYIAEGVADAVGTESDILVNEARERGPVELLHAVERHGRAVRDNDFRYLLL
                     DAGGRRLAGTLPQAAARVGWSKARVAALDEEGAHMIDVLTRGVRLSDGALLVVAGDLE
                     DLRELRARLLQFTALAAVVVVVLALVGGGMAGGLFLRRLDAVNAAIARIIQGNFAQRL
                     PALGISPEFNALTSHLNLMLDRMEALVDSMRQISVDVAHDLRTPLTRLRHRLERIEAS
                     PAGAAVAGDLGGCIAEIDEVIAIFAAMLRISAVESGMAGAGHARIDLSALLGQLVAAY
                     APAIEDGGRRLALRIAPGVHIAADRELLAQAVANLLENAIRHTPPGSTIALGLDGAGL
                     PGMARITVEDDGPGVPEADRERVLRRFFRLERSRTTPGSGLGLALVAAVVRLHHGTIV
                     LEEAAPGLRVRLDLPIAAEGG"
     gene            complement(13083..13706)
                     /locus_tag="VFF98_11810"
     CDS             complement(13083..13706)
                     /locus_tag="VFF98_11810"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZU64363.1"
                     /translation="MRMIKCLALASALTIAHPAAAKTWVSDPVGTWLELHFKHSNLFE
                     VTTSVPNMIATVDLDGEDLTKAHFEISAAVSAMWSHNTLFIGDLKSDQYFWADRYPVV
                     TFSSDRVVKEGTGYRVEGQLTARGVTRPFAMAMVPSKIVPWDGVQFRAFTLNGVLHPK
                     DFGMPFQNPPGLVNDPLFDNQFALTFTVEVTDKPNASAYTPLPSVPH"
     gene            complement(13758..16100)
                     /locus_tag="VFF98_11815"
     CDS             complement(13758..16100)
                     /locus_tag="VFF98_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64364.1"
                     /translation="MRTNHRLPARGRAHRDLPLLLGTALLALAPAAPALANDKTETGA
                     QNPPDTGADSGQIVVTATKRKESQLEVPISMSAISANALQQRQITSFEDVARSVPGLA
                     ITDQEAAGRSSYAVRGVSSAGGVGSIGQQTVGVYLDDVSMTMPTGASVGAATPAFFDI
                     NHMELLRGPQGTLYGAGAMGGAIRVIANQADLARTSLDVRADVSANARGGLGYLGEGV
                     INLPLIRDHLAARVGIQYNHTGGYIDRKDGTTGAITPNFNDSSQLVVRGQLRYENDGG
                     DLQVVPSIFYQKTTTQGSGESDAGNANLVTRYTDEPLRDEIFAPSLTIRKNFGGYALT
                     SATTYLKRSFYTATDYTISALGNELYGLVTPVSMALPTQISQVSEEMRLVSPKANGVD
                     RKFEWILGVYFEHSDTHVANYLTAEENQTSLYDRLAAAYGADTADALTASGSNVYSQI
                     STQSTTQFSGFGELSFKPNRRITATVGFRALTAHQSALTLTNGLIGTGSNSWSSDSSS
                     LNSSAFTPKFSLRYELSRDANIYATAVKGFRLGGINQSVPTSTCGSALGQLGLTKAPM
                     TYKPDSVWSYEGGFKGSLLHHRLDVAGAVYLIDWNGVQQTVNLQTYDNACPYTYVGNV
                     GRARVMGTDLEFSAHLTRTLTLRANGGYTQAEIRTDNESTGTYRGEWLLGVPRWTGAA
                     GFSYNGRADERTRLFGALDVHLIGPSRASFSASDVGYRRSPYATLDGSFGVDMAGTRV
                     SLFAKNITNSRKITQQETASPYLVRALAPRTIGLSVQKSF"
     gene            complement(16138..16776)
                     /locus_tag="VFF98_11820"
     CDS             complement(16138..16776)
                     /locus_tag="VFF98_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZU64365.1"
                     /translation="MPIRFRTALPALLITALLPAAAAQASPAPWYHDPAGCFLELSYV
                     HAGMYRIPVIFPGVSAQARIDPANLPASSFDITVQTASLQVYEPLVHQQMTGNIGLLE
                     PARYPQMRLVSRRIVKTARGYDVWMDTTIHGISHPVRFAMVASPDVPFEGTHFRSFTL
                     RGAISRKAFGMGWMQPEPLKWPLFGDRFDLDLTVELTDRPFGPPPAKPEPHA"
     gene            complement(16940..17395)
                     /locus_tag="VFF98_11825"
     CDS             complement(16940..17395)
                     /locus_tag="VFF98_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allene oxide cyclase family protein"
                     /protein_id="HZU64366.1"
                     /translation="MSKAGLVLALAANGLAANALAAPAHSAPLRHLDLVERAATDTVS
                     LHGGAGADNVGDVLTFANPVYDAGNTQVLGQDHGYCVRLVVGKSMECHWTLMLQHGQI
                     MADGPVYDSADTLMAITGGTGAYRGVRGEVLIHVRDAKATAYDFHYRLQ"
     gene            complement(17464..18921)
                     /locus_tag="VFF98_11830"
     CDS             complement(17464..18921)
                     /locus_tag="VFF98_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU64367.1"
                     /translation="MSDMTSQDDAELSALGYTAHFDRNMSLWENFALGFTYLSPVVGV
                     YSVFGIGMAAGGPAMIWWYPIAALGQFIVCLTFCEVVSQFPISGGLYPWCRRLVGRKW
                     AWMAGWIYGWALFTSIAAVAAGAAPFVAPLLGFQSSPVLAGLIAIALVALSTLTNLAG
                     TRMLARVAMFGFICEILGALAVGTFLLLFHRHQPLGILFSSFGIARDGHYGPAFLSAA
                     IVGLFTCYGFEACGDVAEETPNPGIAIPRAMKSTIYIGMSASIFVCLALILSLPDVMA
                     VVEGRVANPIEDALLAAFGPLGAKLVMLVVLVSFLSCILSLQAAASRLVYAYARDDMV
                     VASGWLARVSERHHVPTNALVLSGVVAGAILVLGMFFADTVATIVSFAVVGIYAGFQM
                     VVLGALVARSRGWRPRGRFRLGAWAWPVNVAGLAWGVWAIADMLRPADGAGPWYMSWA
                     MALTFGAVVLTGLAYMLAARPHDRSDAPAGDAWQV"
     gene            complement(18977..20389)
                     /locus_tag="VFF98_11835"
     CDS             complement(18977..20389)
                     /locus_tag="VFF98_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HZU64368.1"
                     /translation="MRIGDRLETGSEEPERLINPRTGATIAEIAEASFAQVDQAVAAA
                     QAAFAPWARTTPAERSNLLLKLADLVERQADEYARLEALNCGKPLHLVLGDEIPAVVD
                     CLRFFAGAGRAMQGPLAGEYLPGHTSMIRRDPVGVIAAIAPWNYPLLMAVWKLAAPVM
                     AGNTVVLKPSELTPLTSFKLVEDAAGLFPPGVINIVYGRGETVGNHLINHPAVGMISV
                     TGDIATGRKVLAAATKTIKRTHLELGGKAPVIVLADADIAEVVQAVRSFGYYNAGQDC
                     TAACRIYCAAPLYERLVADLSAAVATLRFDEADDGLNDMGPLISGRQRSRVAAFVARA
                     AEHGHIEITTGGQPGAGDGFYFQPTVIAGAHQDDEIVRREVFGPVVSITRFTDVEEAV
                     GWANDSDYGLASSVWSKDVGQAMRVAAGLRYGCTWINTHFMLANEMPHGGFKQSGYGK
                     DMSLYALEDYTCPRHIMIKL"
     gene            complement(20649..21563)
                     /locus_tag="VFF98_11840"
     CDS             complement(20649..21563)
                     /locus_tag="VFF98_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64369.1"
                     /translation="MALSLRQIQYFVAIADLGSLSRAAKTLNVTPSSITVSIHDLEES
                     LACRLFERRARGMELTIKGHHFLRHARAILDAVAQARHSLVSDEAPVSGPLNLGVTPL
                     VAGYVLAELLSRFRRSFPNCHVSIIEDTREDLEHLLVGGELDAAAIILPDGLQGQALQ
                     AMLIQSSPYRLWMAADHPLARQAEIDIADLGDTPQILLATDEIAETAEKHWRRLRARL
                     NVLVRTGSVEAVRSLVATGAGVAAMPDLVYRRWSLEGDRVEARTLTHAPTPAQVSVVW
                     RRGAVESPELKGFLSVVRGSPIVGADLA"
     gene            21827..22600
                     /locus_tag="VFF98_11845"
     CDS             21827..22600
                     /locus_tag="VFF98_11845"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64370.1"
                     /translation="MPAHDALCAYGLEGKTALVTGAGSGIGLATARLFAEVGAQVLFV
                     DIAADAVRAAAEAAGSPWAICDIADEAQVDALFAHAQSLFGRLDVLANVAAYRRKADT
                     MAMPAAEWDAMHAVTTRGTFLMMRAAIRLMRAQQGGGAIVNVSSVSADHPTIFNNMHY
                     DSAKAGVDAMTRAAAVEFAGQGIRVNSVQPGGTASGGAGEMAAQLPIRGPMVQPGRIP
                     LGRMSQPIEQARAILFLASDAASYITGHHLTVDGGYHVS"
     gene            complement(22781..22855)
                     /locus_tag="VFF98_11850"
     tRNA            complement(22781..22855)
                     /locus_tag="VFF98_11850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22821..22823),aa:Val,seq:cac)
     gene            22962..23270
                     /locus_tag="VFF98_11855"
     CDS             22962..23270
                     /locus_tag="VFF98_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HZU64371.1"
                     /translation="MTETPETAAQPATSGSDVPPDRLAINPSSTHFDGDLLRRGIGIR
                     FKGVVRTNVEEYCISEGWVRVQAGRSMDRKGNPLTIKLNGPVEAWFEDLGEGAPVASA
                     "
     gene            complement(23331..23951)
                     /locus_tag="VFF98_11860"
     CDS             complement(23331..23951)
                     /locus_tag="VFF98_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HZU64372.1"
                     /translation="MTGWTRSIARGASLALALAATTPAIARVHFVDVSDFNEVGGDER
                     TLPAYLQCVPYARTASGIQLFGDAWTWWRQAEGRYARGVRPKVGAVMAFHPYGKMVLG
                     HVAMVSRIVDSRKVLLRHANWSPINGRRGQVERDVEAVDVSPANDWSEVRVWFAPIQG
                     LGSTHWPVDGFIYNKTPITGAHGLGRNRWSTDPIGAIIAAFTGKND"
     gene            24099..25481
                     /gene="der"
                     /locus_tag="VFF98_11865"
     CDS             24099..25481
                     /gene="der"
                     /locus_tag="VFF98_11865"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZU64373.1"
                     /translation="MLPQVIIIGRPNVGKSTLFNRLVGKKLALVDDQPGVTRDRRFGE
                     AHLLGLDFTIVDTAGWEDEDPHSLPGRMRQQTEAALEGADVALFVIDSRAGLTPLDEE
                     IARYLRESDVPVILVANKAEGRAGDAGLYEAFGLGMGDPVPFSAEHGEGLADLFEALL
                     PLIEDKQPDEDAEPAEEEDEEALLKAPLKLAIVGRPNAGKSTLINALLGEDRLLTGPE
                     AGITRDSIAIDWQWRDPRTGDSRPVRLIDTAGMRKRAQVTDKLEKLSVADARRAVDFA
                     EVVVLLLDATRGLEHQDLKIAAHVIEEGRALMIAVNKWDVAEEASALFNGIRGALEEG
                     LSQVKGLPLLTVSARTGKGLDALIGAAFELREAWSKRVPTALLNRWFEEALTANPPPA
                     PGGKRIKLRYITQAKTRPPGFVLFGTRLDDLPASYQRYLVNGIRRELGFASVPVRLTL
                     RSPKNPFKRD"
     gene            complement(25653..26912)
                     /locus_tag="VFF98_11870"
     CDS             complement(25653..26912)
                     /locus_tag="VFF98_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HZU64374.1"
                     /translation="MPAQDAPAGARLATLDLVRGVAVLGIVAVNMAGFAGPIATTDTP
                     ALAVPPQAGPAGMVDEALFALTMLLVEGKMRALFSMLFGASMLLFVERAELRGRSGEA
                     LQMRRLFWLGAFGYAHYVLLWWGDILFLYALCGLLALALRGLAPRAQVGLAVALFMGW
                     HGWGMIGSLPQILAEEHVRTGIATAREAADVQAALAQTLAQLRADVAQAALGFWPQAA
                     AKLADAPAWPLVMTLYTIGETLPLMLIGMALYASGFFTGHWPARWHRAVLWAGAAGAV
                     WTGVLLGWAWPRHFPPLAMPDVLSYWAGPAHLAMALAYASLLVRAAPCLLGTGPGRRL
                     VAAGRMAFSNYLCTSLAMCFLFNGWGLGWMGHVGRAGQAGLVLLVWAAMLGWSQPWLA
                     RFRQGPLEWLWRSLTEGRPMPFARPNG"
     gene            complement(26921..28072)
                     /locus_tag="VFF98_11875"
     CDS             complement(26921..28072)
                     /locus_tag="VFF98_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU64375.1"
                     /translation="MNRPKRAASPAHRLLAAAGRVGTGLVGAGLAALLMAGAPARADV
                     NTDVNAGVNAGAEAWSRGDFAAAVHAWQGPAETGDAVAQYNLGQAYKFGKGVPQDLTR
                     AEMLFAKAAAQGHAGAADQYGLLLFQRGQHAQAMPYIRTAADRGEPRAQYLLGVALFN
                     GDSIGKDWVRAYALELLANQAGLPQARAALAQMDQFVPLDQRQKGVSLAGQMQPAIES
                     SRNRQTAAELLGADAPPPPATPAPSPRPSVVAALPLPPRASPADAGADYARPKGGQHL
                     AAAPAPAKAPAPAKPAPEKPTPEKPIAAKPAAHGEWMLQLGAFAVAGNADALWARVKA
                     RPEVAGHARENRPTGKVSRLLVTGYSAEGAQGACHKLAAAGIACLPTRN"
     gene            28324..29667
                     /locus_tag="VFF98_11880"
     CDS             28324..29667
                     /locus_tag="VFF98_11880"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU64376.1"
                     /translation="MQQTDAFRFAAAQPGPRWLAAGGALALALGGSLLAVPAFASSEA
                     GRGEAAKAEAAQAAALGGAVSAAEAAVTSAPREAALRAALGRAYFRAGRFESAAAAYG
                     DALTLGDASGRTALSLALSDVAIGRKGEAVQVLDGARGEIGVADFGLAMALAGETGRG
                     VAVLVDAVRSGQGTLQLRQNLAYAYALDGRWEDARVMALLDVPADQVDERMTQWARTT
                     RDDAFRARVASLLGVPLRGDNGMPAQLALAPAPVGQPAPQMALAPTPAPAPAPVAAPA
                     QELPPVNEAPAAAPDDLRRAEAAPAVPAYRPPVFHPVVAEAAPAPRVLAHGMAPARAA
                     VPHGARKPASAGSHVVQLGAFASAENAEKARRQFMAHGAFAGHSIEVARAEVNGRAFW
                     RVSARGYDAASAQGACGALKRHGAVCLARADLGGAQHAAALHGHGTPDLAFADRR"
     gene            complement(29746..30981)
                     /locus_tag="VFF98_11885"
     CDS             complement(29746..30981)
                     /locus_tag="VFF98_11885"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZU64377.1"
                     /translation="MKPVASITITNGLLVLAEGLPVPGALRIEGDRIAALGADVAPAE
                     GERVLDAAGKLIAPGLVDLGVFAIDKPALHFGGITRAALMPDQNPPLDRPSAVRYAAQ
                     SGKPDLWVHPLAAATRALEGRELAEIALMRDAGARAIATGRRWIGDSGTMLRLLRYAA
                     MLDLVVITHAEDAGLVGSGVATASEMATRLGLPSAPAEAEALAIARDVALAEMAGARL
                     HIRQVTTRAGLDLVRAAKARGVAITAGVTPAHFMLSDTALADFRTFCRLSPPLRHEAD
                     RQAVIEAIGDGTIDIIGSGHDPRGPEDKRLPFADAEPGMAGAETLLPLTMNLVRDGVI
                     DMARAFQLLAGNPACILGVNAGRLAPGAEADIAIVDADKPWIVSSDKMAAAAGNTPFD
                     RQPVQGRVLALFKGGVKVG"
     gene            complement(30978..31985)
                     /locus_tag="VFF98_11890"
     CDS             complement(30978..31985)
                     /locus_tag="VFF98_11890"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZU64378.1"
                     /translation="MTSATSINGPYPAGSAAFPHRGLLGIAGLAPHELLFLLDEAEQW
                     VELNRQPQKRADLLQGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMSVGQSSVKKG
                     ETLIDTAVTLNAMRADAIVIRHASSGAVRLIAGKVDCPVLNAGDGQHEHPTQALLDAL
                     TIRRALAERGLGSGFVGLKVVICGDILHSRVARSNILSLTALGADVRVCAPPALMPAE
                     IERMRVTAFHDFDAALKGADVVMMLRLQQERMEGQFIPSPREYRHLYGLTAERLAKAA
                     PGALVMHPGPMNRGIEIDSHIADLAGRSLITSQVEMGVAMRMACLEVLTRKARKLEGW
                     A"
BASE COUNT         5109 a        10468 c        11397 g         5252 t
ORIGIN      
        1 cgatcgaccg cgcggcggcc catttcggca gcgatggccg gctcgtcaac cagctgggca
       61 atggcctggg ccagcgcctg ggcatcgccc ggaggcaccg ccaggccgca gccgatcact
      121 tcctcgtaaa ggcccgtgcc cggctctgcg gtggccacca ccggccggcc cgaggccagc
      181 atgttggtga gcttggaggg cagcaccaga tcggccgccc cggggatctg cggcagcagg
      241 tgcacgctgg ccaggctgag cagatcgttc agccgttcca gcggctgcag atcgtggaac
      301 tggatattgt cgagcccgcc ggccagctcc atcagccggg cgcgattggg cccctcgccg
      361 cagatgacaa aggcgatatc cgtgcgcccg gccagcaggc gggcggtttc caccaggatc
      421 tcgatgccct gcttgttggc gatattgccg gaatagaggc acaccttgcg cccgcccagc
      481 ccccactgcg cgcgatagcc gctttcctcg ggcgggccat agctgccgcg ggcattggcc
      541 cagttgcgga tttccaccac ttcctcggcg gcaaagccct tctcgcgcag cttcacgcac
      601 atttgcgggc tgatcgagga ggcgcggtcg gccaggcgca ggatgcgctg ttccatccgc
      661 cgcgccagcc atttcagcca gcccctgccg cccacaaggc ccgtggcaaa ggccgcttcc
      721 acctcgaaat cctgcacatg cacccacagc cgcgcgccgg ccaggcgggc catcagccag
      781 gccaccggca cgctcagcag cgccggggcc acgcacagca ccacctgcgg gcgctcctca
      841 cgcgggcggc gcgccagcat cagcgccggc cccagcgccg acagcgcaaa gctgatcaga
      901 tggacgatcc ggcgcaggcc attgggttcg cgcggcacat agagcgggca gcgcatgatc
      961 cccacgcccg cctcgcccga gcggcgccag cccccgcgcc aggcctgcgg cacctgccag
     1021 gccggataat agggcttgcc cgccaccact tccaccacat ggccgcgcgc ggccagttcc
     1081 acggccatat cggtggtgta gcgggcgatg ccgatttcct cgggcgcata gttgatgccc
     1141 agaatggcaa ggcgcattgc agatgacatt ctcatccttc gttcatccgt tttgtcgcct
     1201 acccgttacg gttaaggggt aatactagca ttcatgcccc cgccgcgcca agcctgcttc
     1261 gcgcatgcag cacgcaggcc ctggcgggct ggttatttct gggctttcag gaaatgttcg
     1321 taggcatcgg cgatgccggc ttccagcggg atggccggct tccagcccat ggcgtgcagg
     1381 cggctggaat ccatcagctt gcgcggcgtg ccatcgggct tgctggcatc gcgcgtgact
     1441 tcgccggtga agcccaccgt gcgggccacc agatcggcca gatcgccaat cgtcacatcc
     1501 tcgccatagc cgatgttgat cggcagcgga tcggaataat gctgcaggag gaagatgcag
     1561 gcatcggcca gatcgtcggc atgcatgaat tcgcggcgcg gcgtgccggt gccccagatc
     1621 tcgaaactgt cgtgcccggc cagccttgcc tcatgcgcct tgcggatgag ggcgggcagc
     1681 acatggctgg aattgagatc gaaattatcg cccgggccat acaggttggt gggcatcgcc
     1741 gagatgaaat ccaccccata ttgctggcga taggcctggc acagcttgat gccggcgatc
     1801 ttggcgatcg cataccattc gttggtcggc tccagcgggc cggtcagcag gctttcctcg
     1861 cggatcggct gctcggcgaa tttgggatag atgcaggacg agccgaggaa cagcagcttt
     1921 tgcgcaccag ctacgcgggc cgcctcgatg aggttggcct cgatcatcag attgtcgtag
     1981 aggaaatcgg ccggataggt ggaattggcg agaatgccgc ccacccgggc cgcggccacc
     2041 accactacct cgggccgctc gcgcgccatc caggcgcgca cctcggcctg ttcgataagg
     2101 ttcatcgcct cgcgcccggc ggtcagcacc tcgcagttct cgcggcccag ccggcgcacc
     2161 acggcgctgc ccaccatgcc acgatggccg gccacgaaca cccgctttcc tgcaagcgaa
     2221 aaggtcatcg gatcctaatc ctcaattgat cgaaacaaca tgcgccacgc ggcaatggcc
     2281 agcgtgcaag gcacagctgg ccattggtcc tgtcaacatc ggcccggcgc ggccgggttc
     2341 agctgccctt ggcgatcggc gcgtcgcgca tcaccatcag gtcggcctgc accatttcgc
     2401 gcgccagatc gcgcggcgag gtctggtggc tccagcccag cttctcgcgc gccttggtgg
     2461 gatcgccgat cagcagttcc acctcggtcg ggcggaaata gcgcgggtcg atctcgatca
     2521 ggcacttgcc ggtcttctgg caatagccct tctcgtcgat cccctcgccg cgaaattcca
     2581 gatcgatgcc cacatcctcg aaggcccagc gcacgaattc acgcacgcgc gtcgtctcgc
     2641 cggtggccag cacgaaatca tcgggctcgt cctgctgcag catcatccac atgccgcgca
     2701 catattcgcg ggcatggccc cagtcgcgct gggcatcgag attgcccaga tacagctttt
     2761 cctgccggcc cagcgaaatg gccgcggccg cgcgggtgat cttgcgggtc acgaaggttt
     2821 cgccgcgcag cgggctttcg tggttgaaga ggatgccgtt gcaggcaaac atgccatagg
     2881 cctcgcggta gttcaccacg atccagtagc catagagctt ggccacgcca tagggcgagc
     2941 gcggatagaa cggcgtcgtc tcgcgctggg gcacttcctg caccaggcca tacagttccg
     3001 aggtggaagc ctggtagaag cgggtcttct tctccagccc caggatgcgg atcgcctcca
     3061 gaatgcgcag cgtgcccacg gcatcggcat tggcggtgta ttccggcgtc tcgaagctga
     3121 ccagcacgtg gctttgcgcg gccagattgt agatttcgtc gggctgcacc tgctggatga
     3181 tgcggatgat attcgtcgaa tccgtcatat cgccgtaatg aaggtgaaaa cgcgcaccct
     3241 ccacatgcgg atcctgatag atctcctcga tccgcccggt gttgaacgaa gaggagcggc
     3301 gcttgatgcc atgcacctca tagcccttgt ccagcagcaa ttgtgccaga taggcaccat
     3361 cctgcccggt aatgccggta atcagcgcta ccttcttgtg cttcgtctca accatctggg
     3421 ttccggcctg tcctaacggg ggtcaattac gacgatacac ctaatgataa ataccaatat
     3481 agcaaccgcc ttcgccactg caacatagca aaatctcttc gcttctaacc atatgtaacc
     3541 aacctgggcg gccgggtcgg ggccgccgcg cggccaggat gaacaggcgg cgacaattgt
     3601 attcagccgc gcacaagccg cccgcggcta tctggcaggc atcggcgatg ggccggtgcg
     3661 ggagatgcga catgcggcgt ttgacgggtg tgacaggcgg gatggcgggt ttgctggtgg
     3721 ccgggctggc gatggccggc agtgcccagg cgcaggattg gcgcggctat gacgggccgc
     3781 cgccctatgc ccgaggctgg caggagggcg aggggctgcg gatgatagac ctcgtctgct
     3841 cgggccggcg cgcgcatatg ctggaggcgc ggctgggcca tgaggtggag gagggcgaca
     3901 tcgaccccga cactgccggg cgcatgcatg atgcgatcga cgggctggaa gggcgccagc
     3961 gccgggaatg cgcggaaggc gatgtgcgct cggtgcgcga gatcggctgg cgctacgacc
     4021 ggatcggcca gtggatggaa gcggccgccc atggccatga gggctggcgc gattggcggc
     4081 cgggctgggg ggcgcgctag agcgcctgct ccagcccgat gaacaccccg cgccgggcgg
     4141 cccattgcgg ctggccgccg gcaaggccgg tgccatcctg ccagcgccgg gcgccatcca
     4201 gcgcgttcag cacatcgagg cggatatcga gcgggcggcg gccccagccg gcaaaccgcc
     4261 gcaccgccgc caggttcagc gccacccagg ccggcatggt ggcgccattg ggcgcaaggg
     4321 cagtggcgct gcggggcagg ccgctgccga aagtggcatc gcccgaaagg cgccagggcc
     4381 ccgtgcgcca ggccagcccc gccgatccgg tgaagcgctg atcgctgttg gtggccacgg
     4441 cctggctggc cgcccctgcc agcgcggcgg cgctgaacgg tgcctgcccg ctgacgatgc
     4501 gccagccctg cagccgcgaa accgccagat tgccccaggc cgacaagggc ccctcgctcc
     4561 agttggcgct gagttccacg cccttgccgg tgccgcgggc atagttgaag gcatgggcca
     4621 ccagcccgtc ggcgctccac gtctgatcga gcagattgcg ggagccgcgc cagaaggcat
     4681 cgatgcccag cgccagcccg cccagcttct gggtgaggcc gatgtcgatg taatcgtcgc
     4741 gctcggcgcg ggcgggggtg tcggtggcgg tgggcagggc ggcgctgctg ccggcatagg
     4801 cggcgggcgc tatcgcctcg cgctcttgcg gggccggcac gaaatagcgg gcatagccca
     4861 gatggaaggt ggtggccttg gcggggctcc acaccaggct ggcgcgcggg cccggcgcgc
     4921 tgccgccgcc gctgccgctc acccgatcga agcgcaggcc atagttgaac gtgaggcccg
     4981 gcgctgcggc ccattcatcc tgcaggtaaa ggccggtttc ctggcggcgg gcgctggcct
     5041 ggccgcgcag tgtcaggggg ctgtcggcca gcagcacggt atctgcggcg cggctgcggg
     5101 tgcgccgcca gctggccagc aggccggcgc gcagatggtg cgcgcggccc ggcgcataga
     5161 cggcctctgc ctgcaggccg ctgctcaaca gatcggcgcg atcctgccgg gcgatgccgg
     5221 caagggcgag atcgcccggc acatcgccct ccagccgctg cacgccatgg ctgaggaagg
     5281 cggccagctg caggttgagc gccccggcgc tgcgcagata ggaaagcagg ccatactgac
     5341 tggcgcgctg ctgcgccccg cccgctgccg tgccggtgat cgtgccggtg gtcgtgccgg
     5401 tgatcgtgct ggcgggcccg ctgcgggagc ggggcgtttg cagcccgtcg tcggaaacgc
     5461 caaggatcag cgccaggcgc gagcgggcat cgagcaggcg ctcgaaatag gccagcgcgt
     5521 cgccctggtc ggtgtggtcg tggagcgggg tggcgctggc atcgggcgcc ggcagcccgg
     5581 catcggagcg cagccaggcg cccgagacat aatagctggt gcccgcctcg gcgccggcca
     5641 gttcgaaggc cggctcgaag cgcccctgcg tgccgccata cagttccacc tcgccgtcat
     5701 gctgatagac gccgctcttg gtggtgaggt tgacgatgcc gccggtctgc agcccatatt
     5761 gtgccggcag cgcgccggtg accagctcca cccggtcggc aaagcgggcg cgcagcgtgt
     5821 cggccgggtc tgccagccca tcgggcagga tgatgttgtt gaggcgatat tgcacatgat
     5881 cctgcccgcg cacgcggatc gccccatcgc ccccttgcga gacgccgggc atctgcagca
     5941 gcacattcac cagattggct gtttcgccgc tgggtcgctt ttccaccgcg tcattcgtca
     6001 gcagataggc cgaggcgccc agcgccgggt tgatgctggc ccgcgctgca tcaagctggc
     6061 gcgcggtgac gacgatcgcg ccggtctgca ccgggccgtc gtcgccatcg tcgtcatcgt
     6121 cgtcatgcgc cgggcgctgc ggggcatggg gcgcgggcgc tgcatgggcg atggcgcccg
     6181 gggccggcag gccggcggat ccggccgcga ccatgcaggc caggcccaga tgaagaattt
     6241 gccccagacg cacgcgctcg ctcccttgga tggtgttgcc ccgcctccct ctggcctgcg
     6301 cattaaggca gtattaggag gctgggacgg caagagggga agggcgccat gcgggtgctg
     6361 gtggtggaag atgaggggcg gatcgccgag ctggtgagcg cggccctgcg cgcggcgggc
     6421 tttgccgtgg atgtctgcgc cagcgcgggc gatggcgccg ccgcgcagga actgaccgcc
     6481 tatgacgcgc tggtgctcga tctgggcctg cccgatggcg atgggctggc ggttttgcgc
     6541 gcggcaaggc ggggcggggg cagcgtgccg gtgctgatcc tcaccgcgcg cgatgccgtg
     6601 gaagaccgcg tggccgggct tgacgccggg gcggacgact atctcaccaa gcccttcgcc
     6661 acggccgagc tgatctcgcg catcaaggcg ctgctgcgcc ggccggggct ggcgctgggg
     6721 gccacgctgg cagccggagg gctgttgttc gacacgctgg gccgcgaggc gcaggtggct
     6781 ggccgcccgc tggcgctggc gcggcaggaa ctggctatcc tggaacatct gatgcgccgg
     6841 ctgggccggg tggtgccgcg cgatgtgctg gaggaaaagc tctatggcga gggcggcgag
     6901 atcgcctcca acgccattcc ggtgcatatc caccacctgc gccgcaagct ggccgatgcc
     6961 ggcgccggct gcgccatcca gacggtgcgc ggcatcggct attttctggc ggaggagcgg
     7021 gcatgaccgg ccgctggcgg gcatcgctgg aagggcggct gatcctgcgg ctggtggcgg
     7081 cctgcctggc cgcttcggtg gccggttttg ccgggctggt gatcaagagc tggcgcatcg
     7141 cccggggcct ttccgatgag ggcgcggcgg atattttcgt tggcgaattc ttgaaggagg
     7201 cgggctgggc ctttccgctt ttcgcgctgc tgatcatcac actcgtggtg gccacggtgc
     7261 gctctgccct gcgcccgctg gcggctgccg cgcagcaggc cgctgccatc gccccggcgc
     7321 gggccgacag gcgcctgccc gccgctggcc tgcccggcga gatcctgccc ttcgttggcg
     7381 cggtgaacgc cgcgctggag cggctggagc agggcttcga ggcgcagcgc cgcttcaccg
     7441 ccgatgccgc gcatgaactg cgcacgccgc tggcgatcct gatcgccggg ctggaggcgc
     7501 tgcccgaagg ggcgccagtg gccgccttgc gggccgatgc cgcgcgcatg gggcggctgg
     7561 tcgatcaatt gctgcgggtg gcgcggctgg atgccttgcc ggcgccggtg ctggtgcgcc
     7621 aggatctggc ggcgggcgcg gccggtctgg tggcggcgat ggcgccctgg gcccatgggc
     7681 aggggcgctc tctggcgttc gaggcgccgg ccggcccggc ctgggtgcag gccgatgccg
     7741 acagcctggc cagcgcgctg cgcaacctga tcgagaatgc ggtgttccat gccccccgtg
     7801 gcggcgaggt gctggtgcgc gtggaagggg cctgcgtgcg ggtgatcgac cggggcgccg
     7861 gggttgccgc cggggcgagc gagcgattgt tcgaccgctt ctggcgcgca gccgataccg
     7921 ggcgccatgg ggccgggctg gggctggcga tcgtcgccgg cgtggcccag gcccatggcg
     7981 gccgcgcctg ccacgaggaa accccgggcg gcggcgccac ctttgtgctg cagctggtgc
     8041 ccgccggccc ggcagaaggg tgatccctgc tgccgggcgc ggcggggcgg gggtgcgtca
     8101 gaacttcagc ttcacgccgg tttccagggt cagatcatag aattcgcgct ggatcggccg
     8161 gttgggcgaa ccctcgtaga agcgcagcgg cgcgttggtc aggttcttca tgttgagata
     8221 gagctgcacg cggcggctga tatcgtagga gccgctgaaa tccagcgtgt agcggctgtc
     8281 ctggaagaca tcggtggcgc gcgtggagcc gaccccgaac agcaccgtgc cctcgaactg
     8341 gctggccagg cgcgccttca gcttgccgcg ctcgtagaac acggcagcgt tccacgacca
     8401 cgggatggtg cccggcatgg cgacattgcc ctcgccattg cgcagggcca cgctgctggc
     8461 cacataggtg atgttggcat cgatgccaaa gccgcccagc cagcccggca ggccgcggaa
     8521 atgatcgacg aaattgccct cgatgccgcg cgcatgggca tgatgggcgt tctcgtaggt
     8581 gttcaccaca taggtgtagc cagagccggc ataattgccc accggcaccc cgcgcgtgac
     8641 gatgaagtcc agcagttcct tgtcgaacag gccggccgag acgatgccgg cgccgggcag
     8701 gtaatattcg gccgagaggt cgaaattatg gctgtaggtg ggcagcaggt tggggttgcc
     8761 ggtgctcacc gtctgcgcgc cggtgtcgat gctggtggat tgctgggtct gatagaagcc
     8821 ggggcgggcg aggccggtgg aataggtggc gcgcagcacc agatgggcat cggcctgata
     8881 gtgcagctgc agggtgggga agacattgtc gtaattgtgg cgggtgctca gcggcccggt
     8941 cacggtcgtc accgtggtgc tgctgccgct gccgctgacg acggcggtgt tgccaatgcc
     9001 gcgataggtg gcgctggtgt gttcgaagcg cagccccgcc agcacgccca gcttgccgaa
     9061 atcgcccttg tattgcacga aagcggcggt gatatcctca ttgtcgtcaa aataattgcc
     9121 ggccacctgg gtcagggcgg tgccgctggc gttgaagatg tcgcgcatgg cctggccggc
     9181 gatcatcggg ccgatgttat agccgttgcg gtagaaatcg gtgatcgggc tgctgccggc
     9241 atattggctg aggctggccc cgttggtgat atccagcgtg ccggcatagg aattggccac
     9301 cttgtggcgc cagcgctgct tcacgccgat cttcacctcg tcgttggcgg ccaggcccac
     9361 cggcagggcg ccgctcagcg cataggacca ttcctcgtcg cggtcgtgct cggtggcatt
     9421 gccgatcttg gtgagggcat aattggcagg atcggtgaag cccgtgcccg aggtgacggc
     9481 ataggtggga tagttggggt tggtggtgtt gtcgtaggtc agcgtgtagt tgttggcgtt
     9541 ctcgaaggtg gaattatagt cgaaatgctt gtcgtaggtg gcgcgggaat aggcgcccca
     9601 ataatcgaca tgcagcttgc ccagctggtt gtcgccgccc agctgcagca ccaggttgcg
     9661 gtgggtttcg tcctcgtcgc gcagggtctt caccgggttc accgtatcgg ccaccagccc
     9721 gttggcattg gccggattgg tggcggtgtt gccgttgaag tcgatctcga ggcggttgcg
     9781 atagacatgc tcgttatagc cggccaggct ggcgcgcaga tagtagcgct gggtggcatc
     9841 gggggcatag tccagctcgc ccgaatagcc gaagcgctgg cggtaataat catatttgcg
     9901 catttccagc gcgccatagg ccttgtcggg cgccttgctg gtgtcgtcca catagctttc
     9961 ctcgatatcg tggaagctgc ggtggtcgtt gtagaggaac tggctgacga cgaaggagac
    10021 cgggctgccg gcgccgccga tgggcccgcc cagcaccacc tcgtcgcgat aaaggccctt
    10081 gccggcgaag gtttcgatgc cgccgcccag cgtcacatcg gcaaacaggc ggttggcatg
    10141 aagggcggtg cgcggcgtca gctcgatcga gccgccgatg ccttcggcgt catggtcggg
    10201 caggccggtc ttgatcacgg cgatccggtc gaccgcgccg atcggcacgg tgtcgaattc
    10261 caccgcgcgg ccggcggcgt tgaaataggt gccgcccggc tgggtgttca gcagcaccac
    10321 gccgcccatc gtggcgccat cgaaattgcc gtcgatgccg cggatgttga cgaagcggcc
    10381 ctcggatgtg tcgatcgaca gggagacgcc ggggatgcgg ctgatcgcct cggcggcgtt
    10441 cacatcggga tatttggcga tgctttcggc cgcctggatg ttgatgaggt taggagcgct
    10501 cttctgctcg ctgcgcgcgg cctcgtcgag gcgggccgaa acgatgatgg catccgcctt
    10561 gtttgcggtt gcctcctcgt ctgcatcagc agcaagcccc gcgtgaggca gaagggcgca
    10621 ggccagtgca acaagcgatg cagatgaata gcgcatgatt atgaccccgt tttttaaaaa
    10681 tcggagccgc tattgggcga tggttgttgc acatttggga cggatatatt gattaatcct
    10741 aacactatcc aatgtgttct ggcctgaatt gcgcgttgat atggttttat tttaacgcaa
    10801 acggctcaat ttccggctgt tttcgcccgg cccacagggt gtgccacaag ggcccttggc
    10861 gccggctctc ttccacattg ggaattgcca atcttgcctg ccaccgcctt tcgcacctag
    10921 agcaagcgcc ttgcccaccg ggagggatgg cgtgaagctg ctgctgcttg aggatgacgc
    10981 cgagacgatc gcgcatgtca cccgcatcct gcgggccgat ggccatgtgg tggatgtggc
    11041 gcgcaccggg cccgaggcga tcttcctggc caccaccgcg gcccatgcgg tgctggtgct
    11101 cgaccggatg gtgcccgggc tcgacgggct ggcgacgctg aaggcgatcc gcgcggccgg
    11161 cgtgcagacc ccggcgctgt tcctgaccgc gatgggcgat gtgaccgacc gcgtggaagg
    11221 gctgaatgcc ggcgccgacg attatctggg caagcccttt gccgcctcgg aactgctggc
    11281 gcgcattgcc gcgctgggcc ggcgcacacc gcagatcgat gtcgtcaccc gcatcgtcat
    11341 cgccgatctg gagatcgatc tgttgaagcg gcaggtggcg cgggcggggc ggcgcattga
    11401 attgcagcat caggaatatc ggctgctgga atttctggcg cgccatgccg ggcaggtggt
    11461 cacgcgcacg atgctgctgg aaagcatctg ggatttccac ttcgagccgg gcaccaacct
    11521 gatcgaaagc cacatcagcc ggttgcgcgg caaggtggac aggggcttcg atgtggagct
    11581 gatccgcacg gtgcgcggcg cgggataccg gctcgatgcg ccctgctagg tctggcaggc
    11641 tgcccaccac cggcgctgcg ccgatccggc cgctggccag cggggccttt cgctttgcgc
    11701 tggtgctggc gggggtgttc gcgctcacat cggcggtgct gctgtttctg gtggcgcatc
    11761 tggtcgatca ctacatcgcc gaaggggtgg ccgatgcggt gggcaccgag agcgacattc
    11821 tcgtcaacga ggcgcgcgag cgcggccctg tggaactgct ccatgcggtg gaacgccacg
    11881 ggcgggccgt gcgcgataat gattttcgct accttctgct cgatgcgggc gggcggcggc
    11941 tggccggcac gctgccgcag gcggcggccc gggtgggctg gagcaaggcc cgcgttgccg
    12001 cgctcgacga ggagggcgcg catatgatcg atgtgctgac gcgcggcgtg cgcctctccg
    12061 atggcgcgct gctggtggtg gcgggcgatc tggaggatct gcgcgaattg cgcgcgcggc
    12121 tgctgcagtt cacggcgctg gcggcggtgg tggtggtggt gctggcgctg gtggggggcg
    12181 gcatggctgg cggcctgttc ctgcgccggc tggatgcggt gaatgcggcg atcgcgcgga
    12241 tcatccaggg caatttcgcc cagcgcctgc ccgcccttgg catcagcccg gaattcaacg
    12301 cgctgacctc gcatctcaac ctgatgctcg accggatgga ggcgctggtg gacagcatgc
    12361 gccagatctc ggtcgatgtg gcgcatgatc tgcgcacgcc gctgacgcgc ctgcgccacc
    12421 ggctggagcg gatcgaggcg agcccggccg gcgctgcggt ggccggcgat ctgggcggct
    12481 gcatcgccga gatcgacgag gtgatcgcca tcttcgcggc aatgctgcgg atcagcgcgg
    12541 tggaaagcgg catggccggg gcgggccatg cgcggatcga tctgtcggcg ctgctgggcc
    12601 agctggtggc cgcctatgcg ccggccatag aggatggcgg ccgccggctg gccctgcgca
    12661 tcgcgccggg cgtgcatatc gccgccgatc gcgaattgct ggcgcaggcg gtggccaacc
    12721 tgctggaaaa tgccatccgc cacacgccgc cgggcagcac catcgcgctg gggctggatg
    12781 gcgccggcct gcccggcatg gcgcgcatca cggttgagga tgatggcccc ggcgtgcccg
    12841 aggccgaccg cgagcgcgtg ctgcgccgct tcttccggct ggagcgcagc cgcaccacgc
    12901 cgggctccgg gctggggctg gcgctggtgg cagcggtggt gcgcctgcat cacggcacca
    12961 tcgtgctgga ggaggccgcg ccgggcctgc gcgtgcgcct tgatctgccc atcgcggccg
    13021 aggggggctg aaagcgctgc tggcggcagc ccgaaaccgt cgccgccagc gcgcggggag
    13081 gctcagtgcg gcaccgaggg cagcggcgta taggccgagg cgttgggctt gtcggtcacc
    13141 tcgacggtga aggtgagggc gaactggttg tcgaacagcg gatcgttgac caggcccggc
    13201 gggttctgga agggcatgcc gaaatccttg gggtgcagca ccccgttcag cgtgaaggcg
    13261 cgaaactgca cgccatccca cggcacgatc ttggaaggca ccatggccat ggcaaagggc
    13321 cgcgtcacgc cgcgcgcggt cagctggccc tccacccggt agcctgtgcc ctccttcacc
    13381 acccgatccg acgagaaggt gacgaccgga tagcgatcgg cccagaaata ctgatccgac
    13441 ttgagatcgc cgatgaacag cgtgttatgc gaccacatcg ccgacacggc cgccgaaatc
    13501 tcgaaatggg ccttcgtcag gtcctcgcca tccagatcga cggtggcgat catgttgggc
    13561 acgctggtgg tcacctcgaa caggttgctg tgcttgaagt gcagttccag ccaggtgccc
    13621 acagggtcgg aaacccaggt cttggccgcg gccgggtgag cgatggtcag cgcgcttgcc
    13681 agcgccaggc atttgatcat ccgcatgctg cttccttatc ggttggcggg tgggagtggg
    13741 gcctatctta ccctgattca aaaggatttc tgaaccgaaa ggccgatcgt gcgcggggcc
    13801 agcgcgcgca ccagataggg cgatgcggtt tcctgctggg tgatcttgcg gctgttggtg
    13861 atgttcttgg caaacagcga gacgcgggtg ccggccatgt ctaccccgaa gctgccatcc
    13921 agcgtggcat agggcgaacg gcgatagccg acatcgctgg ccgagaagct ggcccggctg
    13981 gggccgatga gatgcacatc gagcgcgccg aacaggcggg tgcgctcgtc tgcccggccg
    14041 ttgtagctga agccggccgc ccccgtccag cgcggcacgc ccagcagcca ctcgccgcga
    14101 taggtgccgg tgctctcgtt gtcggtgcgg atttcggcct gggtatagcc gccattggcg
    14161 cgcagcgtga gcgtgcgggt gaggtgggcg gaaaattcca gatcggtgcc catcacccgc
    14221 gcccgcccga cattgccgac ataggtgtag gggcaggcat tgtcgtaggt ctgcaggttc
    14281 accgtctgct gcacgccgtt ccagtcgatc agatagacag cgccggccac atccagccgg
    14341 tgatgcagca gcgagccctt gaagccgccc tcatagctcc acaccgaatc gggcttgtag
    14401 gtcatcggcg ccttggtcag ccccagctgg cccagcgccg agccgcaggt gctggtgggt
    14461 acagactggt tgatgccccc cagccggaac cccttgacgg cggtcgcata gatattggca
    14521 tcgcggctca gctcatagcg cagcgagaat ttgggcgtga aggccgagct gttgaggctc
    14581 gagctgtcgc tgctccagct gttcgatccg gtgccgatca ggccgttggt cagggtcagc
    14641 gcgctttggt gggcggtcag cgcgcggaag cccaccgtgg cggtgatgcg ccggttgggc
    14701 ttgaagctca gctcgccaaa gccggagaac tgcgtggtgc tctgggtgga gatctgggaa
    14761 tagacattgc tgccgctggc ggtcagcgcg tcggcggtgt cggcgccata ggcggcggcc
    14821 aggcggtcgt agaggctggt ctggttttcc tcggcggtca gataattggc gacatgggta
    14881 tcggaatgtt cgaaatagac gccgaggatc cattcgaact tgcgatccac gccattggcc
    14941 ttgggcgaga ccagccgcat ctcttccgac acctgcgaga tctgcgtggg cagcgccatc
    15001 gagacgggcg tgaccagccc gtacagctcg ttgcccagcg cgctgatcgt gtagtcggtg
    15061 gcggtgtaga agctgcgctt cagataggtg gtggcgctgg tgagcgcata gccgccgaaa
    15121 ttcttgcgga tggtgaggct gggcgcgaag atctcgtcgc gcagtggctc gtcggtgtag
    15181 cgggtgacga gattggcgtt gccggcatcg ctttcgcccg atccctgggt ggtggtcttc
    15241 tggtagaaga tcgagggcac cacctgcaga tcgccgccat cgttttcata acgcagctgg
    15301 ccgcgcacca ccaattgcga gctgtcgttg aaattgggcg tgatggcgcc ggtggtgcca
    15361 tccttgcggt cgatatagcc gccggtgtgg ttatactgga tgcccacccg cgcggcgaga
    15421 tgatcgcgga tgagcggcag gttgatcacc ccttcgccca gatagccaag gccgccgcgg
    15481 gcattggccg agacatcggc gcgcacatcg agcgatgttc tggccagatc cgcctggttg
    15541 gcgatcaccc ggatcgcccc gcccatggcg ccggcgccat agagcgtgcc ctgtggcccg
    15601 cgcagcagct ccatatggtt gatatcgaag aaggccggcg ttgccgcgcc gacagaggcg
    15661 ccggtcggca tcgtcatcga gacatcgtcg agatagacgc ccaccgtctg ctggccgatc
    15721 gagccgacgc cgccggcaga gctgacgccg cgcaccgcat aggaggagcg ccccgccgct
    15781 tcctgatcgg tgatggcaag gcccggcacc gagcgggcga catcctcgaa gctggtgatc
    15841 tggcgctgct gcagcgcatt ggccgagatt gccgacatgc tgatcggcac ctccagctgg
    15901 ctttccttgc gcttggtggc ggtaacgacg atctggccgc tgtcggcgcc cgtatcaggc
    15961 gggttttgcg cgccggtttc agtcttgtca ttggcgaggg ctggcgcggc cggggccagc
    16021 gccagcagcg cggtgcccag cagcagcgga agatccctgt gcgccctgcc gcgcgcgggc
    16081 agtctgtggt tggtcctcat gtgtcattcc ctttgttttc tatgaatgga ggtaggctta
    16141 tgcgtggggt tctggcttgg ctggcggtgg gccgaagggc cggtcggtca gctccacggt
    16201 caggtcgaga tcgaagcggt cgccaaacag cggccatttc agcggctcgg gctgcatcca
    16261 gcccatgccg aaggccttgc ggctgatcgc gccgcgcagg gtgaaggagc ggaaatgggt
    16321 gccctcgaag ggcacatcgg gcgaggcgac catggcaaag cgcaccgggt ggctgatgcc
    16381 gtggatggtg gtgtccatcc acacgtcata gccgcgggcg gttttcacga tccgccggct
    16441 gacaaggcgc atctgcggat agcgggccgg ttcgagcagg ccgatgttgc cggtcatctg
    16501 ctggtgcacc agcggttcat agacctgcag actggcggtc tgcacggtga tgtcgaagga
    16561 ggaggcgggc aggttggccg gatcgatccg ggcctgcgcg ctgacgccgg gaaagatcac
    16621 cgggatgcga tacatgccgg catgcacata gctcagctcc agaaagcagc cggcgggatc
    16681 gtgataccag ggcgcagggc tggcctgtgc ggcagcggcg ggcagcagcg cggtgatcag
    16741 cagggcgggc agggcggtgc ggaaacggat aggcataagg gcttccccga ggcatcgggc
    16801 ccgtcagata cgggcgcgcg gatgggcggc gcctggcctg gatggcccgg cgcgggatcg
    16861 ggcggcgcac cgcccctggc aggttctcgc aacggttttt acctgccccg cgggccgtca
    16921 gccgcggggc aggggcggct cattgcaggc gatagtggaa atcataggcg gtggccttgg
    16981 cgtcgcgcac atggatcagc acttcgccgc gcaccccgcg ataggcgccg gtgccgccgg
    17041 tgatggccat cagcgtgtcg gcgctgtcgt agaccgggcc atcggccatg atctggccgt
    17101 gctgcagcat cagcgtccag tggcattcca tggatttgcc caccaccagc cgcacgcaat
    17161 agccatgatc ctgccccagc acctgcgtgt tgccggcatc atagaccgga ttggcaaagg
    17221 tcagcacatc gccgacattg tctgccccgg cgccgccatg caggctgacg gtgtcggtgg
    17281 cggcgcgttc aaccagatcg agatggcgca agggggcgct gtgggcgggg gcggccagcg
    17341 cgtttgcggc cagcccgttt gcggcaaggg ccagaaccag gccagccttg gacattctgc
    17401 gcatcattca tgctcctgtg tgggccttgc ggggccggct ggaaagaaag gggaaggaaa
    17461 ggatcagacc tgccaggcat cgccggcggg ggcatcgctg cggtcatgcg ggcgggcggc
    17521 gagcatgtag gccagccctg tcagcaccac cgcgccaaag gtcagcgcca tcgcccagga
    17581 catataccag gggccggcgc catctgccgg gcgcagcatg tcggcaatgg cccacacgcc
    17641 ccaggccagc ccggccacgt ttaccggcca ggcccatgcc cccaggcgaa agcggccgcg
    17701 cgggcgccag ccccggctgc gcgccaccag cgcgcccagc accaccatct ggaagccggc
    17761 atagatgccc accaccgcaa agctgacgat cgttgccacc gtatcggcaa agaacatgcc
    17821 cagcaccagg atcgcccctg ccaccacgcc ggaaagcacc agcgcattgg tgggcacgtg
    17881 atggcgctcg gaaacccggg cgagccagcc gctggccacc accatgtcat cgcgggcata
    17941 ggcatagaca aggcggctgg cggcggcctg caggctgagg atgcacgaca ggaacgagac
    18001 cagcaccacc agcatcacca gctttgctcc cagcgggcca aaggcggcca gcagcgcgtc
    18061 ctcgatcgga ttggccacgc gcccctccac caccgccatc acatcgggca gcgacaggat
    18121 cagcgccagg cacacgaaga tcgaggccga catgccgatg tagatcgtcg atttcatggc
    18181 gcggggaatg gcgatgccgg ggttgggggt ttcctcggcc acatccccgc aggcctcgaa
    18241 gccatagcag gtgaacaggc cgacaatggc cgccgacaga aaggccgggc cataatgccc
    18301 atcccgggcg atgccgaagc tggagaacag gatgcccagc ggctggtgcc ggtggaacag
    18361 cagcaggaag gtccccaccg ccagcgcccc caggatctcg cagatgaagc cgaacatcgc
    18421 cacgcgcgcc agcatccgcg tgccggccag attggtgagc gtggacaggg ccaccagcgc
    18481 gatggcgatc agcccggcca gcaccgggga ggactggaag cccagcagcg gcgccacaaa
    18541 tggcgcggcg cccgctgcca ccgcggcaat cgaggtgaac agcgcccagc catagatcca
    18601 gccggccatc cacgcccatt tgcgccccac cagccggcgg caccagggat agagcccccc
    18661 cgaaatggga aattgcgaga cgacctcgca aaaggtgagg cagacgatga actgccccag
    18721 cgcggcgatc ggataccacc agatcatcgc cgggccaccg gccgccatgc cgatgccgaa
    18781 caccgaatag acccccacca cgggcgacag ataggtgaag ccgagggcga aattctccca
    18841 cagcgacatg ttgcggtcga aatgggcggt gtagcccaga gcgctcagct cggcatcatc
    18901 ctggcttgtc atgtccgaca tgtcttgtgc ttccctgaac cggcggggcg gtctgtgccg
    18961 ccccatccct tttgatttac agcttgatca tgatgtggcg cgggcaggta tagtcctcca
    19021 gcgcgtagag gctcatgtcc ttgccatagc ccgattgctt gaagccgccg tggggcatct
    19081 cgttggccag catgaagtgg gtgttgatcc aggtgcagcc atagcgcagg cccgcggcca
    19141 cccgcatcgc ctggcccaca tccttcgacc acactgagga ggcgaggccg taatcggaat
    19201 cattggccca gccgaccgct tcctccacat ctgtaaagcg ggtgatggag acgaccggcc
    19261 cgaacacctc gcgccgcacg atctcgtcgt cctggtgggc gccggcgatg acggtgggct
    19321 ggaaatagaa gccatcgccc gcgcccggct ggccgccggt ggtgatctcg atatggccgt
    19381 gttcggccgc gcgggccacg aaggcggcca cgcgcgaacg ctgccgcccg gaaatcagcg
    19441 ggcccatgtc gttcagcccg tcgtcggcct cgtcgaagcg caaggtggcc accgccgccg
    19501 acagatccgc caccagccgc tcgtagaggg gggcggcgca atagatccgg caggccgcgg
    19561 tgcaatcctg gccggcgttg taatagccga agctgcgcac ggcctgcacc acttcggcga
    19621 tgtcggcatc ggccagcacg atcaccgggg ccttgccgcc cagttccaga tgggtgcgct
    19681 tgatcgtctt ggtggcggcg gccagcacct tgcggccggt ggcgatatcg ccggtgaccg
    19741 agatcatgcc cacggccggg tggttgatga ggtggttgcc caccgtctcg ccgcggccat
    19801 agacgatgtt gatgacgccc ggcggaaaca ggccggcggc atcctccacc agcttgaaac
    19861 tggtgagcgg ggtaagttcc gacggcttga gcaccaccgt gttgccggcc atcaccggcg
    19921 cggccagctt ccacaccgcc atcagcagcg gatagttcca cggcgcgatg gcggcgatca
    19981 cgcccaccgg atcgcggcgg atcatcgagg tgtggccggg cagatattcg cccgccagcg
    20041 gcccttgcat ggcgcggccg gccccggcga aaaagcgcag gcaatcgacc acggcgggga
    20101 tctcgtcgcc cagcaccaga tgcagcggct tgccgcagtt gagcgcttcc agccgggcat
    20161 attcatcggc ctggcgttcc accagatcgg ccagcttgag cagcaggttg cttctttcgg
    20221 cgggcgttgt gcgggcccag ggggcaaagg cggcctgggc ggcggccacg gcctgatcga
    20281 cctgcgcgaa agaggcctcg gcgatctcgg cgatggtggc gcctgtgcgg gggttgatca
    20341 gccgttcggg ctcctcgctt cccgtttcga ggcggtcgcc aatacgcatc tggatagtca
    20401 tgttcgaacc tctgacagga actgccgttc tggtgcgctg ccgggccggg cggtgagggc
    20461 gcgcaagggc acaccccgcc gcccgccggc aggcggcgct tgcaggaaag ggccggatta
    20521 tcccggccca tcagatgatt gtcagctgat gggagaaggc tatcgaagcg cccctggctt
    20581 cgccattgca aaaataaaaa acacgttttc gtgaaaagcg aatggaggat gccagattgg
    20641 acgccggctc aggccagatc ggcgccgaca atggggctgc cgcgcactac cgagagaaac
    20701 cccttcagct ctggcgattc cacagcgccg cgccgccaca ccacgctgac ctgcgccggc
    20761 gtgggcgcat gggtgagggt gcgcgcttcc accctgtcgc cctccagcga ccagcggcgg
    20821 tagaccagat cgggcattgc cgccacgccg gcgccggtgg ccaccaggct gcgcaccgct
    20881 tccaccgatc cggtgcgcac cagcacgttc agccgggcgc gcaggcgccg ccagtgcttt
    20941 tcggcggttt cggcgatttc gtcggtggcc agcaggattt gcggcgtgtc gcccagatcg
    21001 gcgatgtcga tctcggcctg gcgggccagc ggatggtctg ccgccatcca cagccgatag
    21061 ggcgaggact ggatgagcat ggcctgcagc gcctgccctt gcagcccgtc gggcaggatg
    21121 atggcggccg catccagctc gccgcccacc agcagatgct ccagatcctc tcgcgtgtcc
    21181 tcgatgatcg agacatggca gttggggaag gaacgccgga agcgcgacag cagctcggcc
    21241 agcacatagc ccgccaccag cggcgtgacc cccaggttga gcgggccgct gaccggcgcc
    21301 tcgtcgctga ccaggctgtg gcgggcctgg gccacggcat cgagaatggc gcgggcatgg
    21361 cgcaggaagt ggtggccctt gatggtcagt tccatgccgc gggcgcgccg ctcgaacagg
    21421 cggcaggcca ggctttcctc cagatcgtga atgctgacgg tgatcgagga gggcgtgacg
    21481 ttcagcgtct tggccgcgcg ggacagcgag cccagatcgg caatggcaac aaaatattga
    21541 atttgccgaa gggaaagagc catcctttga tgatatgcaa tggttggggc ctgtcaatca
    21601 tgggcggccc gggccttcgt tcggggagat tgccggcgtg gcaggcggcc cctgcatcac
    21661 ccctgcatca caagggcgat gcatcggccg cttcggcgcg gggcgtgatc gtgtggctgg
    21721 caaatcgggg cagatggctt aagccgcttg tcaggcgtgc cggcaggcgg cgcgcgggtc
    21781 cggcatttcg gcctgccgcg cctgtctgat taaatggaga accgccatgc ccgcccatga
    21841 tgccctttgc gcctatggcc ttgagggcaa gaccgccctt gtcaccggcg ccggatcggg
    21901 cattggcctg gccacggcca ggctgtttgc cgaagtgggg gcgcaggtgt tgttcgtgga
    21961 tatcgcggcc gatgcggtgc gcgcggcggc cgaggcggcc ggatcgccct gggccatctg
    22021 cgacattgcc gatgaagccc aggttgacgc gcttttcgcc catgcgcaaa gcctgttcgg
    22081 gcggctggat gtgctggcca atgtggccgc ctatcgccgc aaggccgata cgatggcgat
    22141 gccggcggcg gaatgggatg ccatgcatgc cgtcaccacg cggggcactt tcctgatgat
    22201 gcgcgcggcg atccgcctca tgcgggcgca gcagggcggc ggcgcgatcg tcaatgtctc
    22261 gtcggtcagc gccgatcacc ccacgatctt caacaacatg cattatgatt cggccaaggc
    22321 cggggtggat gcgatgaccc gcgcggccgc ggtggaattt gccgggcagg gcattcgcgt
    22381 caattcggtg cagccgggcg gaaccgccag cggcggtgcg ggcgaaatgg ccgcgcaatt
    22441 gccgataagg ggcccgatgg tgcagccggg gcgcattccg ctgggccgga tgtcgcagcc
    22501 gatcgagcag gcccgggcga ttctcttcct cgccagcgat gcggccagct atatcaccgg
    22561 gcatcatctg acggtggatg gcggttacca cgtgtcctag cgcgcacgtg tcctggcaag
    22621 ccgggccagg cacaggctgc gcaacagagc cggggcctgc ggtaaatcgt ctgacggtgt
    22681 ttgcagggat cgctggtgct atgcgctatg cccgatcgcc tgactttgcc ggatggttcc
    22741 gggcaaacca gccatctttc cagccaagcg cttgaaaaga tggtgggcgc ggcagggatt
    22801 gaacctgcga ccccacccgt gtgaagggtg tgctctaccg ctgagctacg cgcccgccct
    22861 gatggtgaac cgtcgtttcc ggcggcccct cagtggggag acgcgcctct agaagggttc
    22921 tgctgcgttg acaagtgggt tttgaaaatt attcaggcgg catgaccgaa acacccgaaa
    22981 ccgccgccca gcccgccacc tctggctccg acgtgccgcc cgaccggctg gccatcaacc
    23041 cgtccagcac gcatttcgat ggcgatctgc tgcgccgggg cattggcatc cgcttcaagg
    23101 gcgtggtgcg caccaatgtc gaggaatatt gcatttccga aggctgggtg cgcgtgcagg
    23161 ccggccgcag catggatcgc aagggcaatc cgctgacgat caagctgaat ggcccggtgg
    23221 aagcctggtt tgaggatctg ggcgagggcg cgcctgtagc cagcgcgtga tctgtgccgg
    23281 ggcagggcgg ttcgcccccc tgccctttgg gcccttcggc cactgcgcct tcagtcgttc
    23341 ttgccggtga aggcggcgat gatcgcgccg atcgggtctg tcgaccagcg gttgcggccc
    23401 agcccgtggg cgccggtgat cggcgtcttg ttgtagatga agccatccac cggccagtgc
    23461 gtgctgccca gcccctggat gggggcgaac cacacgcgca cctcgctcca gtcgttggcg
    23521 ggcgagacgt ccaccgcctc cacatcgcgt tccacctggc cgcgccgccc gttgatcggg
    23581 ctccagttgg cgtggcgcag cagcaccttg cgcgaatcga caatgcggct gaccatcgcc
    23641 acatggccca gcaccatctt gccatagggg tgaaaggcca tcaccgcgcc cactttgggc
    23701 cgcacgccgc gggcatagcg cccctcggcc tgccgccacc atgtccaggc atcgccgaac
    23761 agctggatgc ccgaggccgt gcgggcatag ggcacgcatt gcagataggc cggcagcgtt
    23821 cgttcatcgc cgcccacctc gttgaaatcg ctgacatcca cgaagtgcac ccgcgcgatg
    23881 gccggcgttg tggctgccaa ggccagggca aggctcgcac cccttgcgat cgatctggtc
    23941 catcccgtca tgccgccacc ctgcattgcg cagggttagg ctgcccctgc cagacatggt
    24001 aaatgaaaaa cttacgattg acctgaaggt tcccgtaaac atacgcaggc cgcgccgttt
    24061 tgcttgccaa gcggcgccgg ggaagccaca ggcaccagat gctgccgcaa gtgatcatta
    24121 tcggccggcc caatgtcggc aaatccacgc tgttcaaccg gctcgtgggc aagaaactcg
    24181 cgctggtcga cgaccagccc ggcgtgacgc gtgaccgccg ctttggcgag gcgcatctgc
    24241 tggggctgga cttcaccatc gtcgacaccg ccggctggga ggatgaagac ccccacagcc
    24301 tgcccggccg catgcgccag caaaccgaag ccgcgctgga aggcgctgac gtggcgctgt
    24361 tcgtcatcga ttcgcgtgct ggcttgacgc cgctggacga ggaaatcgcc cgatatctgc
    24421 gcgaatcgga cgtgccggtg attctggtgg ccaacaaggc cgagggccgc gcgggcgatg
    24481 ccggcctgta tgaagccttt ggcctgggca tgggcgatcc ggtgcccttc tcggccgagc
    24541 atggcgaggg gctggccgat ctgttcgagg cgctgctgcc gctgatcgag gacaagcagc
    24601 ccgacgagga tgcggaaccg gccgaagagg aagacgagga agcgctgctg aaggcgccgc
    24661 tgaagctggc catcgtgggc cggccgaatg cgggcaagtc aacgctcatc aacgcgctcc
    24721 tcggcgagga ccggctgctg accgggccgg aagcgggcat cactcgcgat tcgatcgcca
    24781 tcgactggca gtggcgcgac ccccggactg gcgatagccg cccggtgcgg ctgatcgaca
    24841 ccgccggcat gcgcaagcgc gcgcaggtga ccgacaagct ggagaagctc tcggttgccg
    24901 atgcgcgccg ggcggtggat tttgccgaag tggtggtgct gctgctcgat gccacgcgcg
    24961 ggctcgaaca tcaggatctg aagatcgccg cccatgtgat cgaggaaggc cgcgcgctga
    25021 tgatcgcggt gaacaaatgg gatgtggccg aagaggcttc cgccctgttc aacggcatcc
    25081 gcggggcgct ggaagagggg ctgtcgcagg tcaagggcct gccgctgctc accgtttcgg
    25141 cgcgcaccgg caaggggctg gatgcgctga ttggcgctgc tttcgagctg cgcgaggcgt
    25201 ggagcaagcg cgtgccgacc gcgctgctca accgctggtt cgaggaagcg ctgaccgcca
    25261 atccgccgcc ggcgccgggc ggcaagcgga tcaagctgcg ctacatcacc caggccaaga
    25321 cgcgcccgcc cggctttgtg ctgtttggca cgcggctgga tgatctgccc gccagctatc
    25381 agcgctatct ggtgaacggc atccggcgcg agctgggctt tgcctcggtg ccggtgcgcc
    25441 tgacgctgcg cagcccgaag aaccccttca agcgcgactg atcgccccgc ctgcgggcag
    25501 ggctggcagg gcggtgtagg ctgggcagtg ctgctcatgg ccgggaacct gcgcggggca
    25561 aaaggaatgg gctgcgcgct gggtcataat atgcgccggc ttgcgggcgt aattcggtaa
    25621 attgcctagt ttgggcgttt ggcggggcgg tttcagccgt ttggccgggc aaagggcatg
    25681 ggccggcctt ccgtcagcga tcgccacagc cattccagcg ggccctggcg gaagcgggcc
    25741 agccagggct ggctccagcc cagcatggcg gcccacacca gcagcaccag cccggcctgg
    25801 ccggcgcgcc ccacatggcc catccagccc agcccccagc cgttgaacag gaagcacatg
    25861 gccaggctgg tgcacagata attggaaaaa gccatgcgcc cggccgccac cagccgccgg
    25921 ccagggcccg tgcccagcag gcaaggggcc gcgcgcacca gcaggctggc ataggccagc
    25981 gccatggcca gatgcgccgg gcccgcccaa tagctcagca catcgggcat ggcgagcggg
    26041 ggaaaatggc gcggccaggc ccagcccagc agcacccccg tccacacagc gcccgcagcg
    26101 cccgcccaca gcacggcccg gtgccagcgc gccggccagt gcccggtgaa aaagccgctg
    26161 gcataaagcg ccatgccgat cagcatcagc ggcagcgtct cgccaatggt gtagagcgtc
    26221 atcaccagcg gccaggcggg ggcatcggcc agcttggcgg cggcctgcgg ccagaagccc
    26281 agtgccgcct gcgccacatc ggcccgcagc tgggccagtg tctgggcaag ggcggcctgc
    26341 acgtcggccg cctcgcgcgc ggtggcgatg ccggtgcgca catgttcctc ggccaggatc
    26401 tgcggcaggc tgccgatcat cccccagccg tgccagccca tgaacagcgc cactgccagc
    26461 cccacctgcg cgcgcggcgc cagcccccgc agcgccagcg ccagcaaccc gcacagcgca
    26521 tagagaaaca ggatatcgcc ccaccacagc agcacataat gggcatagcc aaacgccccc
    26581 agccagaaca gccggcgcat ctgcaaggcc tcgccagagc ggccgcgcag ctctgcccgc
    26641 tccacaaaca gcagcatgct ggccccgaac agcatcgaga acagcgcccg catcttgcct
    26701 tccaccagca gcatggtgag cgcgaacagc gcttcatcca ccatgccggc cggccccgcc
    26761 tgcggcggca cggcaagcgc gggcgtgtct gtagtggcga tggggccggc aaaaccggcc
    26821 atgttcaccg ccacgatccc cagcaccgcc accccgcgca caaggtccag cgtggcaagg
    26881 cgggcgccgg cgggcgcatc ctgggcgggc atgggggcgc ttagttgcgg gtgggcaggc
    26941 aggcgatgcc ggcggcggcc agcttgtggc aggcgccttg cgcgccttcc gccgaatagc
    27001 cggtgacaag cagccggctg accttgccgg tgggccggtt ctcgcgcgcg tggccggcca
    27061 cttcgggccg ggccttcacc cgcgcccaca gcgcatcggc attgccggcc acggcaaagg
    27121 cgcccagttg cagcatccat tcgccatggg cggcaggctt ggccgcaata ggcttttcag
    27181 gggtgggctt ttcaggggcg ggtttggcgg gcgcgggcgc cttggcgggt gccggtgctg
    27241 ccgccagatg ctggccgccc ttgggccggg cataatcggc gccggcatcg gccgggctgg
    27301 cgcgcggcgg caggggcagc gcggcgacaa ccgaggggcg gggcgaaggc gcaggcgtgg
    27361 ccggcggcgg cggggcatcg gcgcccagca gttcggcggc ggtttgtcgg ttgcggctcg
    27421 attcgatggc cggttgcatc tgccctgcca gcgagacgcc cttctgccgc tggtccagcg
    27481 gcacgaactg gtccatctgc gccagcgccg cgcgggcctg cggcaggccg gcctggttgg
    27541 ccagcaattc cagcgcatag gcgcgcaccc agtccttgcc gatgctgtcg ccgttgaaca
    27601 gggccacgcc cagcagatat tgcgcgcgcg gctcgcccct gtcggcggcg gtgcggatat
    27661 agggcatggc ctgggcgtgc tggccgcgct ggaacagcag caggccgtat tggtcggccg
    27721 cgccggcatg gccttgcgcg gcggccttgg caaacagcat ctcggcccgg gtcagatcct
    27781 gcggcacgcc cttgccgaac ttgtaggcct ggcccagatt atattgcgcc accgcatcgc
    27841 cggtttcggc cgggccctgc caggcatgca cggctgcggc aaaatcgccc cggctccagg
    27901 cctcggcgcc ggcgttgacg ccagcgttga cgtcggtgtt gacatcggcg cgggcgggcg
    27961 cgcctgccat cagcagcgcg gcaaggccgg cgcccacaag gccagtgccc acccggccgg
    28021 cagcggcaag caggcggtgg gccggtgagg cggcgcgttt cgggcggttc atgcgtcggt
    28081 tccctgtggg cgcggcaggc gcgcgcggat gaggcgtaaa ggggccggat gtgcccgctc
    28141 gattcaatgg cataggcggc catggttagc aaagccttgc tggccgcgcg cacctgcctg
    28201 aaaagcggcg tgaaacgggg tgtggaacat cgcgttaacc gcaatttagg acagatttgc
    28261 gattcgcata ccggataacc ggaggtttgc ccaagcggca gccccgccaa caggaccgca
    28321 ccgatgcaac agacagacgc tttccgcttt gccgctgccc agccggggcc gcgttggctg
    28381 gccgccggtg gcgcgctggc gctggcgctg ggcggcagcc tgctggcggt gccggccttt
    28441 gccagcagtg aggcgggcag gggcgaggca gcgaaagccg aggcggcgca ggcagcagcg
    28501 ctgggcggcg cggtgagcgc ggcggaagcg gccgtgacca gcgccccgcg tgaggcggcg
    28561 ctgcgcgcgg cgttgggccg ggcctatttc cgcgccgggc ggttcgaatc ggccgccgct
    28621 gcctatggcg atgcgctgac gctgggcgat gcctcgggcc gcaccgcgct cagcctggcg
    28681 ctcagcgatg tggcgatcgg ccgcaagggc gaggcggtgc aggtgctgga tggcgcgcgc
    28741 ggcgagatcg gcgtggccga ttttggcctg gccatggcgc tggccggcga gacgggccgc
    28801 ggcgttgccg tgctggtcga tgcggtgcgc tctggccagg gcacgctgca gctgcgccag
    28861 aacctggcct atgcctatgc gctggacggc cgctgggaag atgcccgggt gatggccctg
    28921 ctcgatgtgc cggccgatca ggtggacgag cgcatgaccc aatgggcgcg caccacccgt
    28981 gacgatgcgt tccgcgcccg cgtggcgagc cttttgggcg tgccgctgcg gggcgataat
    29041 ggcatgcccg cgcagctggc gctggcgccg gccccggtgg gccagcccgc gccgcagatg
    29101 gcgctggcgc caacgccggc gccggctcct gcaccggtgg ccgcgccggc gcaggaactg
    29161 cccccggtga acgaagcgcc cgccgctgcg cccgacgatc tgcgccgcgc cgaggccgcc
    29221 ccggccgtgc cggcctatcg cccgccggtg ttccacccgg tggtggcaga ggccgccccg
    29281 gcgccgcgcg tgctggcgca tggcatggca ccggcccgtg ctgctgtgcc ccatggcgcc
    29341 cgcaagccgg ccagcgccgg cagccatgtg gtgcagctgg gcgcctttgc cagcgccgag
    29401 aatgccgaga aggcccgccg ccagttcatg gcccatggcg cctttgccgg ccacagcatc
    29461 gaggtggccc gggccgaagt gaacggccgt gctttctggc gggtgtcggc gcgcgggtat
    29521 gatgcggcct cggcgcaagg ggcctgcggg gcgctgaagc gccatggcgc ggtgtgcctg
    29581 gctcgcgccg atctgggcgg ggcgcagcac gccgctgccc tccatggcca tggcacgccc
    29641 gatctggcct ttgccgaccg gcgctgacag cggtttttcc gaccacaaaa aagcggggga
    29701 gggcaaacct ctcccgcttt tttgtgggcc ggttgcgggc gggcctcagc ccaccttcac
    29761 gccgcccttg aacagcgcca gcacccggcc ctgcaccggc tggcggtcaa acggggtgtt
    29821 gccggcagcg gcggccatct tgtcggaaga gacgatccag ggcttgtcgg catcgacgat
    29881 ggcgatgtcg gcctcggcgc ccggggccag gcgcccggcg ttcaccccca ggatgcaggc
    29941 cgggttgccc gccagcaact ggaaggcgcg ggccatgtcg atcacgccgt cacgcaccag
    30001 gttcatcgtc agcggcagca gggtttccgc gccggccatg cccggttcgg catcggcaaa
    30061 gggcaggcgc ttgtcttccg gcccgcgcgg atcgtggcca gagccgatga tgtcgatcgt
    30121 gccatcgccg atcgcctcga tcactgcctg ccggtccgcc tcgtgccgca agggcggcga
    30181 gaggcggcag aaggtgcgga aatcggccag cgccgtgtcc gacagcatga aatgcgccgg
    30241 cgtgaccccg gcagtgatcg ccaccccgcg ggccttggcc gcgcgcacca gatcgagccc
    30301 ggcgcgcgtc gtcacctggc ggatgtggag gcgcgcgccg gccatttcgg ccagcgccac
    30361 atcgcgcgcg atagccagcg cctcggcctc ggccggggcg gacggcaggc ccaggcgggt
    30421 ggccatctcg ctggcggtgg caacgccgct gcccacaagg ccggcatcct cggcatgggt
    30481 aatgaccacc agatccagca ttgcggcata gcgcagcagc cgcagcatcg tgcccgaatc
    30541 gccgatccag cggcggccgg tggcaatggc gcgggcgcct gcatcgcgca tcagcgcaat
    30601 ctcggccagc tcgcgccctt ccagcgcgcg ggtggccgcg gccagcggat gcacccacag
    30661 gtccggcttg ccgctctgcg cggcataacg cacggcagag ggccgatcga gcggcgggtt
    30721 ctgatcgggc atcagcgcgg cgcgggtgat gccgccgaaa tgcagcgccg gcttgtctat
    30781 cgcgaaaacg cccagatcaa ccaggcccgg cgcgatcagc ttgccggccg catcgagcac
    30841 gcgctcgccc tcggccgggg ccacatcggc gccaagggcg gcaatgcggt cgccctcgat
    30901 ccgcagggcg cccggcaccg gcaggccttc tgccagcacc agcaggccat tggtgatggt
    30961 gatgcttgcc accggtttca tgcccagccc tccagcttgc gtgccttgcg ggtcagcact
    31021 tcgaggcagg ccatgcgcat ggccacgccc atttccacct gcgaggtgat cagcgaacgg
    31081 ccggcaagat cggcgatgtg cgaatcgatc tcgatgccgc ggttcatcgg ccccgggtgc
    31141 atcaccagcg cgccgggcgc agccttggcc aggcgctctg ccgtaagacc ataaaggtgg
    31201 cgatattcgc gcggggaggg gatgaactgg ccctccatcc gttcctgttg caggcgcagc
    31261 atcatcacca catcggcgcc cttcagcgcg gcgtcgaaat catggaaagc ggtgacgcgc
    31321 atgcgctcga tctcggccgg catcagcgcc gggggcgcgc acacgcgcac atcggcgccg
    31381 agcgctgtga gcgagaggat gttcgagcgg gccacgcggc tgtgcagaat atcgccgcag
    31441 atcaccacct tcagcccgac aaagccgctg cccagcccgc gttcggccag tgcccggcgg
    31501 atcgtcagcg catcgagcag ggcctgggtg gggtgttcat gctggccatc gccggcgttc
    31561 agcaccgggc aatccacctt gccggcgatc agccgcaccg cgcccgagga ggcatggcgg
    31621 atcacgatcg catcggcgcg catggcgttg agcgtgacgg cggtgtcgat cagcgtctcg
    31681 cccttcttca cgctcgactg gccgaccgac atgttcacca catcggcccc cagccgcttg
    31741 ccggcaatct cgaaggacag cagcgtgcgg gtggagttct cgaagaaggc gttgatgatg
    31801 gtgaggccct gaagcaggtc ggcgcgcttt tgcggctgac ggttcagttc cacccattgc
    31861 tcggcctcgt cgagcaggaa gaggagctcg tgcggggcaa ggccggcaat gccgagcagc
    31921 ccgcgatgcg ggaaggccgc cgatcctgcc ggataggggc cgttgatgga agtcgcagat
    31981 gtcatcgaag atcaggccat aggggcggtg ggcggcggcc tcaagcccac tttgcctctt
    32041 tgtccaccgt gtttaagaag gggcgttggc cagctggcgc agatggggat gaaagacatg
    32101 gaattcgccg ggaaagtctg gcgcttgctg gtgggcgtga aggatgcgct ggtgctgatg
    32161 gtgctgctgc tgttcttcgg cctgctgtat gccgcgctgg ccgggcgggc gccggcgccg
    32221 gcgcag
//
LOCUS       DASRYE010000229        18549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_119796,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000229 DASRYE010000000
VERSION     DASRYE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119796"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFF98_11895"
     CDS             complement(<1..1140)
                     /locus_tag="VFF98_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZU64379.1"
                     /translation="MSAYVFHFGGGAAPQDPRARDKTIVGGKGANLAEMASIGLPVPP
                     GFTISTEVCAYYNANGKSFDADLRAGVAAGVAHVESATARSFGDAANPLLVSVRSGAR
                     VSMPGMMDTVLNLGLNDATVLGLAAGSGDERFAWDSYRRFIQMYSDVVLGIDHHLFED
                     ALEIVKEDNGFYADIELGAGHWQDLVGQYKAIVESELGRPFPQDVHEQLWGAIGAVFD
                     SWESDRAKVYRRLNDIPADWGTAVNVQAMVFGNMGDTSATGVAFTRDPATGERAYYGE
                     WLVNAQGEDVVAGIRTPQSLTRAAREEMGDANPSMEEALPEVFVQFKAVVERLERHYR
                     DMQDIEFTVQQGKLYMLQTRNGKRTAKAALKVAVDMAGEGLITQKE"
     gene            complement(1190..3262)
                     /gene="glyS"
                     /locus_tag="VFF98_11900"
     CDS             complement(1190..3262)
                     /gene="glyS"
                     /locus_tag="VFF98_11900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZU64380.1"
                     /translation="MSTFLLELFSEEIPARMQAGARAELERLFRAGLAAAGVAAGALT
                     VWSTPRRLALIARDLPAATQAVREEVKGPRSSAPAQALEGFLRKTGLTPDQLTERDGV
                     YFAITEKPGRALAEVLAEMVPAIVRGFAWPKSQRWGAPSLSSESPRWVRPLRGIIALL
                     DGAVVPCEVAGVAAGRATLGHRFHATGAITVADADSYAGQLRAAHVIVDHEERAGIVR
                     ARAQEAAAAAGLTLVEDEGLVVENAGLTEWPVPLLGRFDEAFLAVPPEVIQLTARINQ
                     KYFICRDGAGKLANAFICTANIAARDGGAGIVDGNRKVLAARLSDARFFWEQDLKVPL
                     ARQAEKLARITFHEKLGTLADKVARVAQLAEWLAAEGIVPGADPVLARQAAELCKADL
                     VTEMVGEFPELQGLMGGYYAAAEGLDAAVAEAIRDHYKPVGQGDDVPTAPVTVAVALA
                     DKLDTLVQFFAEDLRPTGSKDPFALRRAALGILQILATNKMRVRLFGVINATAITRLG
                     NYHQRVVDNSLADEVMSFFIDRLKVQQKEAGVRHDLIDAVFALGGEDDLVRLLARVHA
                     LQGFVATEDGANLLAGYKRAANILKAEEKKGWADGALNAAPEPAEAALATALAEAAPR
                     AEAAVAAEDFTGAMAALASLRASVDAFFDGVIVNDADPAKRDARLTLLAKLRAAVHKV
                     ADFSRIEG"
     gene            complement(3259..4173)
                     /locus_tag="VFF98_11905"
     CDS             complement(3259..4173)
                     /locus_tag="VFF98_11905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZU64381.1"
                     /translation="MTNAADSARAPLSFQDMILTLHAYWSRQGCLILQPYDMRVGAGT
                     FHPATTLRSLGPEPWNAAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPENLQEL
                     YLGSLAAIGIDPLAHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVTQFTYFQQVGGFD
                     CKPVSGELTYGLERLAMYIQGVDRVYDLRFNDMGVSYGDVFLANEQEFSKYNFEVADT
                     EQLFIGFQHAEKEAQRCIAANIPLAAYDQAIEASHLFNLLQARGVISVQERASYIGRV
                     RDLAKGAAVKLIEKNEARWAAQFPGWTK"
     gene            4285..5211
                     /locus_tag="VFF98_11910"
     CDS             4285..5211
                     /locus_tag="VFF98_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZU64382.1"
                     /translation="MLPFFNRFARYLLLAGLLLAPLSPPAMARALPRHGAAPAAIAAP
                     HPALWRLRFGASTIYLFGTVHLMSAEADWFAPPVARAFAASDTLVTEIIEPAPQEMAR
                     LLLARATLPQGQSLRALLPAKTRATYEQALAGIHLPPAALDRFQPAFGAVSLVTLSLK
                     QAGFAPDNGVDAQLAARADAAHKAREALETVDFQLGLFASLPMDKQQAYLAEVLDNLP
                     KLPTDMRDVVAAWKAGRVARLARLINDDTDDPALKQALLINRNKAWAQWLKARRLQPG
                     TVFVAVGAGHLAGPGSVIDQLAALGIRAERLP"
     gene            5208..6086
                     /locus_tag="VFF98_11915"
     CDS             5208..6086
                     /locus_tag="VFF98_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZU64383.1"
                     /translation="MRRPRPAWRAAALALCLLAGACTKPDPASPALWRVDGPNGAHGW
                     LFGTIHALPHKAAWRTPAVADALDQSDRLVVEIAGLNDGAALSRAFRALSEDRAHALP
                     PLADRVAPEQRAALADLVKRGGLAAGALDSAKTWAAAIVLGQIAASAEDAAHGIDRDL
                     IAHYPHAIGELEGAPAQFALFDALPEEAQRRLLADTLAGAPTAEADGRQLAAEWRAGD
                     MGAIAAETRRGLLADPTLHEALYAARNRRWAAAIEAQMKGGAHPFVAVGAAHLAGAEG
                     LPALLAADGWQLRRVE"
     gene            6216..6503
                     /gene="rplY"
                     /locus_tag="VFF98_11920"
                     /pseudo
     CDS             6216..6503
                     /gene="rplY"
                     /locus_tag="VFF98_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
     gene            6634..7206
                     /gene="pth"
                     /locus_tag="VFF98_11925"
     CDS             6634..7206
                     /gene="pth"
                     /locus_tag="VFF98_11925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZU64384.1"
                     /translation="MQIWVGLGNPGEQYRLNRHNVGFMAADVIAGVHRFGPEQKKFSG
                     WLREGRIGTEKVLLLKPATFMNESGRAVAEAMRFYKLGLDALTVFHDELDLAPMKVKV
                     KQGGGIAGHNGLRSIAQHLGPDFRRVRIGIGHPGHKDRVTAHVLGNYAKAEMGLLEDM
                     LGAMAAESEWLAKGDDTRFMNDVALRLQQA"
     gene            7231..8331
                     /gene="ychF"
                     /locus_tag="VFF98_11930"
     CDS             7231..8331
                     /gene="ychF"
                     /locus_tag="VFF98_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZU64385.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTETQAAQAANYPFCTIEPNVGQ
                     VGVPDPRLDKLAAIAGSQKIIPTQLSFVDIAGLVRGASKGEGLGNQFLGNIREVDAIV
                     HVLRCFENDDIQHVDNRVDPISDAETVETELMLSDLESLEKRVPAAQKKAAQGDKEAK
                     ITASVLGQALELLRDGKPARLTEPKDDDEARIFAQAQLLTAKPVLYVCNVEEESAASG
                     NAFSEKVFAKAKAEGATAVIVSAAIEADLVGMDTAERTEFLAELGLAETGLARIIRAG
                     YELLGLLTFFTVGPKEARAWTVACGSRAPQAAGAIHSDFERGFIRAETIAFDDYIALN
                     GEAGARDAGKLRQEGKDYVVKDGDVFHFKFNV"
     gene            8346..8804
                     /locus_tag="VFF98_11935"
     CDS             8346..8804
                     /locus_tag="VFF98_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZU64386.1"
                     /translation="MRFYEDLVVGEAEHFGQHAVLREEVIAFARQFDPQPFHLSDEAA
                     AQTHFGRLAASGWHTCGMTMAMLVAHFQETGHQGLGGAGIENLAWLQPVYPGDTLSVE
                     VVVTEKRRSRSRPEMGIIRQQTTTFNQHGEPVLRYTATGMIATRDPAGQD"
     gene            complement(8815..10653)
                     /gene="mutL"
                     /locus_tag="VFF98_11940"
     CDS             complement(8815..10653)
                     /gene="mutL"
                     /locus_tag="VFF98_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZU64387.1"
                     /translation="MEPARPTIRRLPDALINRIAAGEVVERPASALKELMENALDAGA
                     AQLVVRLAEGGLAGIEVVDDGCGMAPAEIALALERHATSKLPDEHIERVLTMGFRGEA
                     LPSIASVARLQVDSRVRGAGEGWRRVVDHGALVEEGPVGLPPGTRIRVDGLFARVPAR
                     RKFLRSARSEYAACLDVVRRLAMARPEVGFTLEHDGRRVLAVQKGETAPQRVARIVAR
                     ELAEDGVVIDLERGPVRLTGVAGLPTFNRGVADHQYLFVNGRPVRDRLLVGAVRGAYA
                     EMLARDRHAVLALFLDVPPEEVDVNVHPAKTEVRFRDPAFIRGFIVSGLRHALDAAGQ
                     KTAQSPAAPAMANWQVEPLAGQGAPAPARQLESLFARQYAPQPAFARPRVEEAASALW
                     RGYEGDVLAPAGKAEEAPPAPEAASYPLGVARGQVGTTYIIAEAADGLVIVDQHAAHE
                     RIVLERLRAAREAAGAEAAPAQALLLPQVVEMDEPDCDRLEEAAATLAGFGLALERFG
                     PAAMLVRAVPAALARGDASALVRDIADDLAQNGAALLLPERLDHVLATMACHGSVRAG
                     RQLSVAEMNALLREMEATPRSGQCNHGRPTWVKLAHGDIEKLFGRK"
     gene            10900..11946
                     /locus_tag="VFF98_11945"
     CDS             10900..11946
                     /locus_tag="VFF98_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZU64388.1"
                     /translation="MSSLMSRFFKFGSQNMAIDLGTANTLVYVQDRGIVLNEPSVVAI
                     ETINGNKSVKAVGDDAKMMMGKTPDNIEAIRPMRDGVIADIEIAEEMIKHFIRKVHGK
                     KSLFRYPEIVICVPSGSTSVERRAIRDAASNAGASQVYLILEPMAAAIGADMPVTEPV
                     GSMVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAIVSYVRRHHNLLIGEATAER
                     IKKDYGIATIPADGVGETIHLKGRDLVNGVPKEITITQANVAEALSEPISAIVEGVRI
                     ALENTQPELAADIVDQGIMLTGGGALIKGLDDFLREETGLPVSVAEDPLSCVALGTGR
                     AMEDPIYRGVLMTA"
     gene            12042..13055
                     /gene="mreC"
                     /locus_tag="VFF98_11950"
     CDS             12042..13055
                     /gene="mreC"
                     /locus_tag="VFF98_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZU64389.1"
                     /translation="MAPPANRRTGYSRRAQYSTFFAYVAGVIGVLAGALLLLGSISNS
                     AAASSPVLAFLRGTAGDITAPLGRAVAAMRASVAQGVDVGQGFLASGSEVARLRKEVA
                     LARVARVETRAAQDENRRLKALLDLATQDPRPIANGWLISSSASSTRRYALVSAGLRD
                     GVRPGMPVRTPEGLVGRVLEVGQVSARVLLITDTQSVVPVRRAQDGVPAFATGKGDGT
                     LVLRLLTLAVNPLHVGDVFVTSGSGGIYWPGTPIAQVTGLTHDGATARVLGDPAASEM
                     VAVLPAWTPTEDATLPPPTGEQPPAPKPTAKAKGKKGAKGAKPAASPAPAPAPAPAGK
                     HAP"
     gene            13052..13609
                     /locus_tag="VFF98_11955"
     CDS             13052..13609
                     /locus_tag="VFF98_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZU64390.1"
                     /translation="MMERLAALLAREGAPRRLNAAPSPAIAYSTPWISIALSSMAVGL
                     PLIAKAPLLPPLGYLALLGWRQLRPGLLPVWAGAALGFVDDLFSGQPMGSAILLWSAC
                     MLALDVIEARFPWRTFLIEWLVAAGMILAYLLATTLLANITGGATPLRLVLPQLLLSV
                     LCFPVAGRVVAALDRWRLGGFRIFG"
     gene            13654..15726
                     /gene="mrdA"
                     /locus_tag="VFF98_11960"
     CDS             13654..15726
                     /gene="mrdA"
                     /locus_tag="VFF98_11960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZU64391.1"
                     /translation="MLRNSFDRRSVVIGALQGGVGVLLATRLGYLAVAENAHYQMMAE
                     SNRVNLSLIPPRRGWILDRQGQPLAANRADFRVDLIPDRLVDADKTLAELGQILGLDA
                     IDIQDIKDQLAQARGFQPVGVAEHLDWEKFAALSVRQPDLPGVVPQRGFSRFYPTGPC
                     VGHLVGYVGAANAKDYEKEHNQLLITPGFKIGKDGLEKQFDADLRGVPGARRAEVTAS
                     GKIVRDLETREDIPGKPIELTIHAGLQEYAARRIGVESGAVTVIDCRSGDILAMCSMP
                     SYDPNSFSAGIGRLEWKMLADDDHVPLRNKTLHGLYPPGSTVKPSVALALLEAGLDPH
                     EAVNCSGGLQVGNRVFHCWNHHGHGRVDMFKGIYQSCDVYFYHFAQKIGMERIAAMMR
                     RMGLGQSFDLPLGKQSTGTVPDPAWKQRRYHQDWAIYDTVNATIGQGYVQVNPLQQAV
                     VVSRLASGKALQPRLLRNQHTPPPGDLGIPQEHFDVIRSGMRDVINGPGTGGKARLPF
                     PDILMAGKSGSAQVVGLNYGNGKAGVAWKRRDHGHFICFAPFDNPRFACAVLIEHGGG
                     SPAAIPIARDVMTYLFDQQKAWDMLATMEPGWGGTPQQRMDAKYRTYAAQYGVSAPRV
                     DTSGEPVAQVQSASDAAAATPQPVQSDAAPPAPEPGAEAPAPAPAATPAAPAPAPAAP
                     GNATGGGH"
     gene            15726..16844
                     /gene="rodA"
                     /locus_tag="VFF98_11965"
     CDS             15726..16844
                     /gene="rodA"
                     /locus_tag="VFF98_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZU64392.1"
                     /translation="MSSRSIIPAPLRQQPWHVLLPLAALNTFGSAVLYSAAGGRMWPW
                     AGMHMLHFAVFLALAMAVSRVPRHLFTQAAFPAYAALIVLLVLVEAVGRIGGGSQRWL
                     NLGFMTLQPSETMKPVIVLVLAHFYANLPPAMIRSWRALVPAGLLMGVPAVLVILQPD
                     LGTALAICFGAVAVIFMAGVPIWWFVSGGLAAGIIAPLAYFFALHDYQRKRVTVFMDP
                     ESDMLGAGYHITQSKIAIGSGGIFGKGFGNGTQSHLEYLPESHTDFAFATMAEEWGLI
                     GGYFILAVFFLVLFRWGMGVARRAPDRFSQLLAAGMTVTIFFYVAINMMMVMGLAPVV
                     GIPLPFISHGGSSMMTNMLCIGMIMAVERWSKRASTLV"
     gene            16984..17059
                     /locus_tag="VFF98_11970"
     tRNA            16984..17059
                     /locus_tag="VFF98_11970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17017..17019,aa:Lys,seq:ctt)
     gene            17104..17179
                     /locus_tag="VFF98_11975"
     tRNA            17104..17179
                     /locus_tag="VFF98_11975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17137..17139,aa:Lys,seq:ctt)
     gene            17299..18015
                     /locus_tag="VFF98_11980"
     CDS             17299..18015
                     /locus_tag="VFF98_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU64393.1"
                     /translation="MTQSPPDDRPPAAEPPAGTDTATASDKVADAILRGIRAGTFATG
                     QHLVEPDLMRRLGISRGSLREALKRLAAEGIVTLNRFRGAYISSFDRKTVFDLLQMLE
                     PMFCLAARLAAEHCDTAEKLARVQAVTASIEAAAARRNRADYMEQRRNFYSVLIEVGG
                     NSELMRAMPLARTELFRAQIEAALSERQRQLHAGGYGRISRAVIERNPAAAERAMKKH
                     FASTHKTISELPDHVFVAAS"
     gene            complement(18043..>18549)
                     /locus_tag="VFF98_11985"
     CDS             complement(18043..>18549)
                     /locus_tag="VFF98_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64394.1"
                     /translation="GLDILANIAGSYPTARVEDTSDAQFAATLAANLGATFAFCRAAA
                     PALRARGGAIVNMSSTAARFPTPGLAVYSASKAGVEGFTRSLAAELAPAVRVNAVAAG
                     PTRTETVDALMASDTTGAARAVTSALPLGRLAEVEEIAGVVAFLASAQAAVITGQVLY
                     ANGGGHMA"
BASE COUNT         2998 a         6452 c         6157 g         2942 t
ORIGIN      
        1 ttccttctgg gtgatcaggc cctcgccggc catgtccaca gcgaccttca gggcggcctt
       61 ggccgtgcgc ttcccgttgc gcgtctgcag catgtagagc ttgccctgct gcacggtgaa
      121 ctcgatgtcc tgcatgtcgc ggtagtggcg ctcgagccgc tcgaccaccg ccttgaactg
      181 gacgaacacc tcgggcagcg cctcctccat cgagggattg gcgtcgccca tctcctcgcg
      241 cgccgccctg gtcagggact ggggcgtgcg gatgccggcc accacgtcct cgccctgggc
      301 gttcaccagc cattcgccgt aataggcgcg ctcgccggtg gccggatcgc gcgtgaaggc
      361 aacgccggtg gcgctggtat cgcccatgtt gccgaacacc atcgcctgca cgttgaccgc
      421 cgtgccccaa tcggccggaa tgtcgttcag ccggcgatag accttggcgc ggtcgctctc
      481 ccagctgtcg aacacggcgc cgatggcacc ccacagctgc tcatgcacat cctgcgggaa
      541 ggggcggccc agttcgctct cgacgatcgc cttgtactgg ccgacgagat cctgccagtg
      601 gccggcgccc agctcgatat cggcatagaa gccgttgtct tccttgacga tctcgagggc
      661 gtcctcgaac aggtgatggt cgatgcccag caccacgtcg gaatacatct ggatgaagcg
      721 gcgatagctg tcccaggcga aacgttcgtc gcccgagccg gccgccaggc ccagcaccgt
      781 tgcatcgttg aggcccaggt tcagcaccgt gtccatcatg cccggcatcg acacccgcgc
      841 acccgaacgc accgaaacca gcagcggatt ggccgcatcg ccaaagctgc gcgccgtggc
      901 gctttccaca tgggcaacgc ctgctgccac gcccgcgcgc agatcggcat caaagctttt
      961 gccgttggcg ttgtaataag cgcagacttc ggtggaaatg gtaaagcccg gaggcaccgg
     1021 caggccaatg ctggccatct cggccagatt ggcgcccttg ccgccaacaa tcgtcttgtc
     1081 gcgggcgcgc gggtcttgcg gcgcggcgcc gccaccgaaa tggaatacgt atgcgctcat
     1141 gagattcacc tttacgaggt gtggaaaggg atgattcgcg gagaagtgcc tagccttcga
     1201 tccgcgagaa gtcagcaacc ttgtgcactg cagcacgcaa ctttgccaga agtgtgagcc
     1261 gcgcgtcacg ctttgccgga tcggcatcgt tgacgatgac cccatcgaag aaggcatcga
     1321 cactggcgcg caggctggcc agcgccgcca ttgcgcctgt aaagtcctcc gccgccacgg
     1381 ccgcctcggc gcgcggggcg gcttcggcaa gggcggtggc aagggcggcc tcggccggtt
     1441 ccggcgctgc gttcagcgcg ccatcggccc agcccttctt ctcctcggcc ttcaggatat
     1501 tggccgcgcg cttgtagccg gccagcaggt tggcgccatc ctcggtggcc acaaacccct
     1561 gcagcgcatg cacgcgcgcc agcaggcgca ccagatcatc ctcgccgcca agggcaaaca
     1621 cagcgtcgat cagatcgtgg cgaacgcccg cttccttctg ctgcaccttc aggcggtcaa
     1681 tgaagaacga cataacctca tcggcaaggc tattatcaac gaccctctga tggtaattgc
     1741 caagacgcgt aattgctgtc gcattaatga caccaaacag gcgaacacgc attttattag
     1801 tggccagtat ttgcaaaatg cccaatgcag cccgacgcaa agcgaaagga tctttcgagc
     1861 ctgtcggccg caaatcctcg gcaaaaaatt gaacaagcgt atccagcttg tcggccagcg
     1921 ccacggccac cgtcaccggc gctgtgggca catcatcgcc ctggcccacc ggcttgtagt
     1981 gatcgcggat ggcctcggcc acggccgcgt ccagcccctc ggcagcggca taatagccgc
     2041 ccatcaggcc ctgcagctcg gggaactcgc ccaccatctc ggtcacgaga tcagccttgc
     2101 acagctctgc cgcctggcgc gccagcaccg gatcggcgcc cggcacaatg ccttctgccg
     2161 ccagccattc ggccagttgg gccacgcggg ccaccttgtc ggccagcgtg cccagctttt
     2221 cgtggaaggt gatgcgggcc agcttttccg cctggcgggc cagcggcacc ttcagatcct
     2281 gttcccagaa gaagcgggca tccgacaggc gcgcggccag caccttgcga ttgccatcga
     2341 caatgccggc gccgccatcg cgggcggcga tattggccgt gcagatgaag gcattggcca
     2401 gcttgccggc gccatcgcgg cagatgaaat acttctggtt gatgcgcgcc gtcagctgga
     2461 tcacctcggg cggcacggcg aggaaggcct cgtcgaaccg gcccagcagc ggcaccggcc
     2521 attcggtaag gccggcgttt tccaccacca gcccctcatc ctccaccagc gtcaggccgg
     2581 cggcggcggc agcctcctgc gcacgggcgc gcacgatgcc ggcgcgctcc tcatggtcga
     2641 cgatcacatg ggcggcgcgc agctggccgg catagctgtc ggcatcggcc acggtgatgg
     2701 cgcctgtggc atggaagcga tggcccagcg ttgcccggcc agcggcaacg ccggccactt
     2761 cgcaaggcac caccgcgcca tccagcaggg cgatgatgcc ccggagcggg cgcacccagc
     2821 gcgggctttc cgaggagagc gagggcgcgc cccagcgctg gctcttgggc caggcaaagc
     2881 cgcgcacgat cgccggcacc atctcggcca gcacctcggc cagggcgcgg ccgggcttct
     2941 cggtgatggc gaaatagacg ccatcgcgct cggtcagctg gtctggcgtg aggccggtct
     3001 tgcgcaggaa gccttccagc gcctgcgccg gcgcgctgct gcgcggcccc ttcacttcct
     3061 cgcgcacggc ctgggtggcc gcgggcagat cgcgcgcgat cagcgccagg cgccgcggcg
     3121 tggaccacac ggtgagcgcg cctgccgcaa cgcccgccgc tgccagcccg gcgcgaaaca
     3181 gcctttccag ctcagcccgg gcgccggcct gcatgcgggc cggaatttcc tcggaaaaca
     3241 gttcgagaag gaaagtgctc acttcgtcca ccccgggaat tgcgcggccc agcgggcctc
     3301 gttcttttcg ataagcttga cagcggcgcc cttggccaga tcgcgcacgc ggccgatata
     3361 gctcgcccgt tcctgcacgc tgatcacccc gcgcgcctgc agcaggttga acaggtggct
     3421 ggcctcgatc gcctggtcat aggcggccag cggaatattc gccgcgatgc agcgctgggc
     3481 ttccttttcg gcatgctgga agccgatgaa cagctgctcg gtatcggcca cctcgaaatt
     3541 atatttcgag aactcctgct cattggcaag gaacacatcg ccataggaca cgcccatgtc
     3601 gttgaagcgc aggtcataga cccggtccac gccctggata tacatggcca ggcgctccag
     3661 cccataggtc agctcgcccg agaccggctt gcaatcgaac ccgccaacct gctggaaata
     3721 ggtgaactgc gtcacctcca tgccatcgca ccacacttcc cagcccagcc cccaggcgcc
     3781 cagcgtgggg ctttcccagt cgtcctcgac aaagcggatg tcatgcgcca gcggatcgat
     3841 gccgatggcc gcaaggctgc cgagatagag ctcctgcagg ttctccgggc tcggcttcat
     3901 gatcacctga tactggtaat aatgctgcag ccggttgggg ttctcgccat agcggccatc
     3961 ggtgggccgg cggctgggct gcacataggc ggcattccac ggctctggcc ccagcgagcg
     4021 cagagtggta gccgggtgaa acgtgcccgc gcccacgcgc atgtcgtagg gctgcaggat
     4081 caggcagccc tgccggctcc aataggcgtg gagcgtgagg atcatgtcct gaaagctcag
     4141 gggcgcgcgg gcgctgtcgg cggcattggt catggcctgc gccttaggcc gatggggcgc
     4201 ttgcggtcaa tgggcggcag ggagaagcgc cggcgcgaca gcgccgcctc gacagcaccg
     4261 caaggcccgc tatagaacag cgccatgctg ccttttttca accgctttgc gcgttatctg
     4321 ctgctggccg gcctgctgct ggcgccgctg tcgccccctg ccatggcgcg ggccctgccc
     4381 cggcatggcg cagcgccagc cgccatcgcg gcaccccacc cggcgctgtg gcgcctgcgc
     4441 ttcggggcca gcacgatcta cctgttcggc acggtgcatc tgatgtcggc cgaggcggac
     4501 tggtttgctc cccccgtggc ccgcgccttt gccgcatccg acacgctggt gaccgagatc
     4561 atcgagccgg ccccgcaaga gatggcccgc ctgctgctgg ccagggccac cctgccccag
     4621 ggccagagcc tgcgcgccct gctgccggcc aaaacccgcg ccacctatga gcaggcgctg
     4681 gccggcatcc acctgccgcc ggccgcgctt gaccggttcc agccggcctt cggggcggtc
     4741 tcgctggtca cgctgtcgct gaagcaggcc ggctttgcgc ccgacaatgg cgtcgatgcc
     4801 cagctggccg cccgcgccga tgccgcccac aaggcgcgcg aggcgctgga gacggtggat
     4861 ttccagctcg gcctcttcgc ctcgctgccg atggacaagc agcaggccta tctggccgaa
     4921 gtgctcgaca acctgcccaa gctgcccacc gacatgcgcg atgtggtggc cgcctggaag
     4981 gccggccggg tggcgcgcct ggcccgactg atcaacgacg acaccgacga tccggccctg
     5041 aaacaggcgc tgctgatcaa ccgcaacaag gcctgggcgc aatggctcaa ggcgcggcgg
     5101 ctgcagccgg gcacggtgtt cgtggcggtg ggcgcggggc atctggccgg ccccggctcg
     5161 gtgatcgacc agctggccgc gctgggcatc cgggccgagc ggctgccgtg aggcggcccc
     5221 gccccgcatg gcgcgcggcc gcgctggcgc tgtgcctact ggccggcgcc tgcacgaagc
     5281 cggatcctgc cagccccgcc ctctggcggg tggatgggcc caatggcgcc catggctggc
     5341 tgttcggcac catccacgcc ctgccccaca aggccgcctg gcgcacgccg gccgtggccg
     5401 atgcgctgga ccagtcggac cggctggtgg tggagattgc cgggctgaac gatggcgccg
     5461 ccctttcccg cgcctttcgc gccctgtcgg aagaccgcgc ccatgccctg ccgccgctgg
     5521 ccgatcgcgt ggcgcccgaa cagcgcgcgg cgctggccga tctggtcaaa cgcggcgggc
     5581 tggccgccgg cgcgctggat agcgcgaaga cctgggcggc ggccatcgtg ctgggccaga
     5641 tcgccgcctc ggccgaggat gccgcccatg gcatagaccg cgacctcatc gcccactacc
     5701 cccatgccat cggcgagctg gaaggcgcgc ccgcccagtt cgccctgttc gacgccctgc
     5761 ccgaggaggc ccagcgccgc ctgctggccg acacgctggc cggcgccccc accgccgagg
     5821 ccgatggccg ccagctggcc gccgaatggc gcgcgggcga catgggcgcc atcgccgccg
     5881 aaacccgccg cggcctgctg gccgatccca ccctgcacga ggcgctctat gccgcgcgca
     5941 accgccgctg ggccgcggcc atcgaggcgc agatgaaggg cggcgcgcat cccttcgtgg
     6001 ccgtgggcgc ggcgcatctg gcgggcgcgg aaggcctgcc cgccctgctc gccgccgatg
     6061 gctggcagct gcgccgggtg gagtaggcgc gcaactgccg cgcgaccttg ccttttggcg
     6121 catttgccgc taagggcgcc cgcttcccgt catggtcatc cctggaggcg tggcgggcga
     6181 tagtgtgttt tccagtgttt tcgaaaggca agacgatgag cgacaagctg accctgccgg
     6241 ccgaggcgcg cgcgaagact ggcaagggag ccagccgcgt tctgcgtcgt gaaggccgcg
     6301 tgcccgtggt ggtttatggt ggcaagaccg agccgctgtc cgcccatgtg gaagagaagg
     6361 aactggtgcg ccagctgggc accggcactt tcttcgacgg cgtggtggaa ctggtgatcg
     6421 atggccagac catcaccacc accgccaagg acgttgcttt ccacccggtg agcgaccgcc
     6481 cgctgcacgt ggacttcctg cgcagctgac gaatgcggcc gccctttgcc gggcggccac
     6541 atggcaaggc gggcaagcgg ccgcaaggcg ctttcccgcc ttttgcttgc ctgatgcccg
     6601 ctgcccgcga aaacgggcct gaaggatggc tgcatgcaga tctgggtggg cctgggcaat
     6661 ccgggcgagc aatatcggct caatcgccac aatgtcggct tcatggcggc agatgtgatc
     6721 gccggcgtgc accgtttcgg gccggagcag aagaagtttt ccggctggct gcgcgaaggg
     6781 cgcatcggca ccgagaaggt gctgctgctg aagcccgcca ccttcatgaa cgagagcggc
     6841 cgcgcggtgg ccgaggccat gcgcttctac aagctgggcc tcgatgcgct gaccgtcttc
     6901 cacgacgagc tcgaccttgc gcccatgaag gtgaaggtga agcagggcgg cggcattgcc
     6961 gggcacaatg gcctgcgctc catcgcccag catctggggc cggattttcg ccgcgtgcgc
     7021 atcggcatcg gccaccccgg ccacaaggac cgggtgaccg cccatgtgct gggcaattac
     7081 gccaaggccg aaatgggctt gcttgaagac atgctgggcg ccatggcagc cgaaagcgaa
     7141 tggttggcaa agggcgacga cacgcgcttc atgaacgatg tggcgctgcg cctgcaacag
     7201 gcctgaacaa ggccggtcac gagggacaag atgggtttca aatgcggtat cgtcgggctg
     7261 cccaatgtcg gcaagtccac cctgttcaac gcgcttaccg agacgcaggc ggcgcaggcg
     7321 gccaactatc ccttctgcac catcgagccc aatgtgggcc aggtgggcgt gcccgatccc
     7381 cggctcgaca agctggccgc gattgccggc agccagaaga tcatccccac ccagctgtcc
     7441 ttcgtggata ttgccgggct ggtgcgcggc gcttccaagg gcgaagggct gggcaaccag
     7501 ttcctcggca acatccgcga ggtggatgcc atcgtccacg tgctgcgctg cttcgagaat
     7561 gacgacatcc agcatgtcga caaccgggtg gacccgattt ccgacgccga gacggtggaa
     7621 accgagctga tgctctcgga cctggaaagc ctggaaaagc gcgtgccggc agcgcagaag
     7681 aaggccgcgc agggcgacaa ggaagcgaag atcaccgcct ctgtgctggg ccaggcgctg
     7741 gaactgctgc gcgatggcaa gcccgcccgc ctgaccgagc ccaaggatga cgacgaggcc
     7801 cgcatcttcg cccaggccca gctgctgacc gccaagcccg tgctctatgt gtgcaacgtg
     7861 gaagaggaaa gcgccgcttc gggcaatgcc ttctccgaaa aggtgtttgc caaggccaag
     7921 gccgaaggcg ccaccgcggt catcgtctcg gccgcgatcg aggccgatct ggtgggcatg
     7981 gacacagcgg aacgcaccga attcctcgcc gagctgggcc tcgctgaaac gggtctcgcc
     8041 cgcatcatcc gcgccggcta cgagctcctc ggcctgctca ccttcttcac cgtgggcccc
     8101 aaggaagccc gcgcctggac ggtggcctgt ggctcgcgcg cgccgcaggc ggccggcgcc
     8161 atccactccg acttcgaacg cggcttcatc cgcgccgaaa ccatcgcctt tgacgattac
     8221 atcgccctca acggcgaggc cggcgcgcgc gatgccggca agctgcgcca ggaaggcaag
     8281 gattatgtgg tgaaggacgg cgacgtcttc cacttcaagt tcaacgtgtg aaggtggcag
     8341 gggcgatgcg cttctacgag gatctggtgg tgggcgaggc cgagcacttt ggccagcacg
     8401 ccgtgctccg cgaggaggtg atcgcctttg cccgccagtt cgatccccag cccttccacc
     8461 tgtcggacga ggcggccgcg caaacgcatt tcggccgcct tgccgcctcg ggctggcaca
     8521 cctgcgggat gacgatggcc atgctcgtcg cccatttcca ggaaaccggc catcaggggc
     8581 tgggcggcgc cgggatcgag aacctcgcct ggctgcagcc cgtctacccc ggcgatacgc
     8641 tgagcgtgga agtggtggtg accgagaagc ggcgcagccg ttcgcgcccg gaaatgggca
     8701 tcatccgcca gcagaccacc accttcaacc agcatggcga accagtgctg cgctacaccg
     8761 ccaccggcat gatcgccacg cgcgatcccg ccggacagga ctgaacctgc cggcctattt
     8821 gcggccgaac agcttctcga tatcgccatg ggccagcttc acccaggtgg gccggccgtg
     8881 gttgcactgg ccggaacgcg gcgtggcttc catctcgcgc agcagggcgt tcatttccgc
     8941 caccgacagc tggcgccctg cccgcacgct gccatggcag gccatggtgg ccagcacatg
     9001 gtccagcctt tcgggcagca gcagggccgc gccattctgc gccagatcat cggcgatatc
     9061 gcgcaccagc gcgctggcat cgccgcgcgc cagcgccgcc ggcaccgcgc gcaccagcat
     9121 ggcggcaggg ccaaaccgtt ccagcgccag gccaaagccg gcgagcgtgg cagcggcttc
     9181 ctccagccgg tcgcaatcgg gctcgtccat ctccaccact tgcggcagca gcagggcctg
     9241 ggcgggcgcg gcctcggcgc cggccgcctc gcgcgcggcg cgcaggcgtt ccagcacgat
     9301 ccgctcatgc gcggcatgct ggtcaacgat gaccaggcca tcggccgcct cggcgatgat
     9361 ataggtggtg cccacctgcc cgcgcgccac gcccagcgga taggaggctg cctcgggcgc
     9421 agggggcgct tcctcggcct tgccggcggg cgccagcaca tcaccctcgt aaccgcgcca
     9481 gagggcgctg gccgcttcct ccacccgcgg gcgggcaaaa gcgggctggg gcgcatattg
     9541 gcgggcaaac aggctttcca gctggcgcgc cggcgccggc gcgccttgcc cggccagcgg
     9601 ctccacctgc cagttggcca tggcgggggc agcggggctt tgcgcggtct tctggccggc
     9661 ggcatccagc gcatggcgca ggcccgagac gatgaagccg cgaatgaagg ccggatcgcg
     9721 gaagcgcact tccgtcttgg ccgggtgcac gttcacatcc acctcttccg gcggcacatc
     9781 gaggaacagc gccagcaccg catggcgatc gcgcgccagc atctcggcat aggcgccgcg
     9841 caccgcgccc accagcagcc gatcgcgcac cggccggccg ttgacgaaga gatactggtg
     9901 atcggccacg ccccggttga aggtgggcag gcccgccacc ccggtcagcc gcacagggcc
     9961 gcgttcgaga tcgatcacca cgccatcctc agccagttcg cgcgccacga tgcgggccac
    10021 gcgctgcggc gccgtctcgc ccttctgcac cgccagcacg cggcgcccgt catgctccag
    10081 cgtgaagccc acctcggggc gggccatcgc cagccggcgc accacatcga ggcaggcggc
    10141 atattccgaa cgggccgagc gcaggaactt gcgccgcgcc ggcaccctgg caaacagccc
    10201 atcaacccgg atgcgcgtgc ccggcggcag gcccaccggc ccctcctcca ccagcgcgcc
    10261 atgatcgacc acccgccgcc agccctcgcc ggcgccgcgc acccggctat ccacctgcag
    10321 ccgcgccacg ctggcgatgg aaggcagcgc ctcgccgcga aagcccatgg tcaacacccg
    10381 ctcgatatgt tcatcgggca gcttggacgt ggcatggcgc tccagcgcca gggcgatctc
    10441 ggccggcgcc atgccgcagc catcatccac tacctcgatg ccggcaaggc ccccttcggc
    10501 caggcgcacc accagttggg cagcgccggc atccagcgca ttttccatca gctccttcag
    10561 ggccgaggcg ggccgctcca ctacctcgcc ggcagcgatg cggttgatga gcgcgtcggg
    10621 cagacggcgg atggtgggcc gggcgggttc catgccccca tcataggccg caaagccgcc
    10681 gctgacaccg ccagacgcgc cccttttcaa ccgaaatcgc tgtatggcgg gcatattggc
    10741 gcaaagctgg cgctgttggg ctataaatcg gcgggcaggc gcgaatcgcg tttgctcccg
    10801 gggcgatgct tgcgctaagg agccggctcc tgcccctcga tagcccggga atttcggcct
    10861 ccctcgccgg aacctccggg aaaactgacg gatttcccaa tgtcatcgtt aatgtcgcga
    10921 ttcttcaagt tcggctcgca gaacatggcc atcgacctcg gtacggccaa cacgctcgtc
    10981 tatgtgcagg atcgcggcat tgtgctgaac gagcccagcg tggtggcgat cgagacgatc
    11041 aacggcaaca agtccgtcaa ggcggtgggc gatgatgcca agatgatgat gggcaagacg
    11101 cccgacaaca tcgaggcgat ccgcccgatg cgcgatggcg tgatcgccga catcgagatc
    11161 gccgaggaga tgatcaagca cttcatccgc aaggtgcacg gcaagaagag cctgttccgc
    11221 tatcccgaaa tcgtcatctg cgtgccctcg ggcagcacca gcgtggaacg ccgcgccatc
    11281 cgcgatgcgg ccagcaatgc cggcgccagc caggtctatc tgatcctcga gccgatggca
    11341 gcggccatcg gcgccgacat gcccgtgacc gagcccgtcg gctcgatggt ggtggatatc
    11401 ggcggcggca ccacggaagt ggcggtgctg tcgctgcgcg gcttggccta caccaccagc
    11461 gtgcgcgtgg gtggcgacaa gatggacgag gcgatcgtct cctatgtgcg ccgccaccac
    11521 aacctgctga tcggcgaggc caccgccgag cgcatcaaga aggattacgg catcgccacg
    11581 atccccgccg atggcgtggg cgagacgatc cacctgaagg gccgcgacct ggtcaacggc
    11641 gtgcccaagg aaatcaccat cacccaggcc aatgtcgccg aggcgctgtc ggaaccgatc
    11701 agcgccatcg tcgagggcgt gcgcatcgcg ctggagaaca cccagcccga actcgccgcc
    11761 gacattgtcg accagggcat catgctgacc ggcggcggcg ccctcatcaa ggggctggac
    11821 gacttcctgc gcgaggaaac cggcctgccg gtgagcgtgg cggaagatcc gctgtcctgc
    11881 gtggcgctgg gcacgggccg ggccatggaa gacccgatct atcgcggcgt gctgatgacc
    11941 gcctgagcgg ccagcacttt ttttccgcgc aaaagcttga atgcacagcc cggccggcgc
    12001 accgttgcgc cggccgggcc aatgaaggga atatcgcaag catggcgccg ccagccaacc
    12061 ggcgcaccgg ctattcgcgc agggcgcaat acagcacatt ctttgcctat gtcgccggtg
    12121 tgatcggcgt gctggcgggc gccctccttc tgctcggctc catctccaac agcgcggcgg
    12181 ccagttcgcc cgtgctggcc ttcctgcgcg gcactgccgg cgatatcacc gcgccgctgg
    12241 gccgggcggt ggccgccatg cgcgcttctg tggcgcaagg ggtggatgtg ggccagggct
    12301 tcctggcttc gggcagcgaa gtggcgcggc tgcgcaagga agtggcgctg gcccgcgtgg
    12361 cccgcgtgga aacccgcgcc gcccaggatg aaaaccgccg cctgaaggcg ctgctcgatc
    12421 tggcgacgca agatccccgg cccattgcca atggctggct gatttcctcc agcgcttcca
    12481 gcacgcggcg ctatgcgctg gtgtcggcgg gcctgcgcga tggcgtgcgc cccggcatgc
    12541 ccgtgcgcac gcccgagggg ctggtgggcc gggtgctgga agtgggccag gtgtcggccc
    12601 gggtgctgct gatcaccgat acgcaaagcg tggtgccggt gcgccgcgcg caggatggcg
    12661 tgcccgcctt tgccaccggc aagggcgatg gcacgctggt gctgcgcctg ctgacgctgg
    12721 cggtgaaccc gctgcatgtg ggcgatgtct tcgtcacctc gggctctggc ggcatttact
    12781 ggcccggcac gccgatcgcc caggtaaccg ggctgaccca tgacggcgcc acggcgcgcg
    12841 tgctgggcga tccggccgcc agcgagatgg tggccgtgct gcccgcctgg accccgaccg
    12901 aagacgccac cctgccgccc cccaccggcg agcagccccc cgctcccaag cccaccgcca
    12961 aggccaaggg caagaagggc gccaagggtg ccaagcctgc cgcctcgcct gccccggcgc
    13021 ctgcccccgc ccctgccgga aagcacgcgc catgatggag cggctggccg cgctgctggc
    13081 ccgcgaaggc gcgccccggc ggctcaacgc cgccccttcg cccgccatcg cctacagcac
    13141 gccctggatc agcatcgcgc tttccagcat ggccgtgggc ctgccgctga tcgccaaggc
    13201 gccgctgctg ccgccgctgg gctatctggc gctgctgggc tggcggcaat tgcgccccgg
    13261 cctgctgccg gtgtgggcgg gcgcggcgct gggctttgtc gatgatctgt tcagcggcca
    13321 gccgatgggc agcgcgatcc tgctgtggtc ggcctgcatg ctggcgctcg atgtgatcga
    13381 ggcgcgcttt ccctggcgca ccttcctgat cgagtggctg gtggccgcgg gcatgatcct
    13441 cgcctatctg ctggccacca ccctgctggc caatatcacc ggcggggcca cgccgcttcg
    13501 gctggtgctg ccgcaactgc tgctttcggt gctgtgcttt ccggtggcgg gccgcgtggt
    13561 ggccgcgctc gaccgctggc ggctcggcgg tttcaggatt ttcggctgat ggcgcgacgg
    13621 cgcggcaaaa ctgccaaaca catttcccag ggcatgctgc gcaacagttt cgaccggcgc
    13681 agcgtggtga tcggcgcgct gcagggcggc gtgggcgtgc tgctggcaac ccggctgggc
    13741 tatctggcgg tggcggaaaa cgcccattac cagatgatgg ccgaaagcaa ccgggtgaac
    13801 ctgtcgctga tcccgccgcg ccgcggctgg atcctcgacc ggcagggcca gccgctggcg
    13861 gccaaccgcg ccgatttccg ggtggacctc atccccgacc ggctggtcga tgccgacaag
    13921 acgctggccg agctgggcca gatcctcggc ctcgatgcga tcgacatcca ggatatcaag
    13981 gaccagctgg cccaggcgcg cggcttccag ccggtgggcg tggccgaaca tctcgactgg
    14041 gagaaattcg ccgccctctc cgtgcgccag cccgatctgc cgggcgtggt gccgcagcgc
    14101 ggcttctcgc gcttctatcc caccgggccc tgcgttggcc atctcgttgg ctatgtgggc
    14161 gcggccaacg ccaaggatta cgagaaagag cacaaccagc tgctcatcac ccccggcttc
    14221 aagatcggca aggatgggct ggaaaagcag ttcgatgccg atctgcgcgg ggtgcccggc
    14281 gcccgccgcg ccgaagtcac ggcctctggc aagatcgtgc gcgacctgga aacgcgcgag
    14341 gacatccccg gcaagcccat cgaactgacc atccacgcag ggcttcagga atatgccgcc
    14401 cggcgcatcg gcgtggaatc gggcgcggtg acggtgatcg attgccgctc gggcgatatc
    14461 ctcgccatgt gctcgatgcc cagctacgat cccaattcct tctcggcggg catcggccgg
    14521 ctggaatgga agatgctggc cgacgacgac catgtgccct tgcgcaacaa gaccctgcac
    14581 ggcctttatc cgcccggctc cacggtgaag ccatcggtgg cgctggcgct gctggaagcc
    14641 gggctcgatc cgcatgaggc ggtgaactgc tcgggcgggc tgcaggtggg caaccgcgtg
    14701 ttccactgct ggaaccacca cggccacggc cgggtggaca tgttcaaggg catctaccag
    14761 agctgcgacg tctatttcta ccacttcgcc cagaagatcg gcatggaacg catcgccgcg
    14821 atgatgcgcc gcatggggct tggccagagc ttcgacctgc cgctgggcaa gcaatcgacc
    14881 ggcaccgtgc ccgaccccgc ctggaagcag cgccgctacc accaggactg ggcgatctat
    14941 gacacggtga acgccaccat cggccagggc tatgtgcagg tgaacccgct gcagcaggcg
    15001 gtggtggtca gccggcttgc ctcgggcaag gcgctgcagc cgcgcctgct gcgcaatcag
    15061 cacaccccgc cccccggcga ccttggcata ccgcaggaac atttcgacgt gatccgctcg
    15121 ggcatgcgcg atgtgatcaa cgggccgggc accggcggca aggcccgcct gcccttcccc
    15181 gatatattga tggccggcaa atcgggctcg gcgcaggtgg tcgggctcaa ctatggcaac
    15241 ggcaaggcgg gcgtggcgtg gaagcggcgc gaccatggcc atttcatctg ctttgccccc
    15301 ttcgacaacc cgcgctttgc ctgcgccgtg ctgatcgagc atggcggcgg ctcgccggcg
    15361 gccatcccga tcgcgcgcga tgtgatgacc tatctgttcg accaacaaaa ggcctgggac
    15421 atgctggcca ccatggagcc gggctggggc ggcacgccgc agcagcgcat ggatgccaaa
    15481 taccgcacct atgccgcgca atatggcgtg agcgcgccgc gcgtggatac cagcggcgaa
    15541 ccggtggcgc aggtgcaaag cgccagcgat gccgccgcgg ccacccccca gccggtgcag
    15601 agcgatgccg cgccccccgc gcccgaaccc ggcgccgaag cgcccgcgcc cgcgcccgcc
    15661 gccacaccgg cagcgccggc accggcgccg gccgcgcccg gcaatgccac cggcggaggc
    15721 cactgatgag cagccgctcg atcatccctg cgcccttgcg gcagcagccg tggcatgtgc
    15781 tgctgccgct ggccgcgctc aacactttcg gctcggccgt gctttattcg gccgcgggcg
    15841 ggcggatgtg gccctgggcg ggcatgcaca tgctgcattt cgccgtgttc ctggcgctgg
    15901 cgatggcggt ctcgcgggtg ccgcggcatc tgttcaccca ggccgccttc ccggcctatg
    15961 ccgcgctgat cgtgctgctg gtgctggtgg aggcggtggg gcgcattggc gggggcagcc
    16021 agcgctggct gaacctgggc ttcatgacct tgcagccatc ggaaacgatg aagccggtga
    16081 tcgtgctggt gctggcgcat ttctatgcca acctgccgcc ggcaatgatc cgcagctggc
    16141 gcgcgctggt gccggcgggg ctgttgatgg gggtgccggc ggtgctggtg atcctgcagc
    16201 ccgatcttgg cacggcgctg gccatctgct ttggcgcggt ggcggtgatc ttcatggccg
    16261 gcgtgccaat ctggtggttt gtctcgggcg ggctggcggc gggcatcatc gccccgctgg
    16321 cctatttctt cgccctgcat gattatcagc gcaagcgcgt gaccgtgttc atggacccgg
    16381 aaagcgacat gctgggcgcg ggctatcaca tcacccagtc gaagatcgcc atcggctcgg
    16441 gcggcatttt cggcaagggc ttcggcaatg gcacgcaaag ccatctggaa tatctgcccg
    16501 aaagccacac cgacttcgcc tttgccacca tggccgagga atgggggctg atcggcggct
    16561 atttcattct ggcggtgttt ttcctggtgc tgttccgctg gggcatgggc gtggcccgcc
    16621 gcgcgcccga tcgcttcagc cagctgctgg ccgccggcat gacggtgacg atcttcttct
    16681 acgtggcgat caacatgatg atggtgatgg ggctggcgcc ggtggtgggc atcccgctgc
    16741 cgttcatcag ccatggcggc tcctcgatga tgaccaacat gctgtgcatc ggcatgatca
    16801 tggcggtgga acgctggagc aagcgggcca gcacgctggt gtaaacgggg gtttgaccag
    16861 gggtttggcc aggcgcgcaa aaaatttata acgaatcact tgcctgcgcc gaaacaggcg
    16921 ctaaagggag cgcctccggt gacggggcgg cggcttcagc ctcctccccc agatggcgga
    16981 aatggacgca tagctcagtt ggtagagcag ctgactctta atcagcgggt cctaggttcg
    17041 agccctagtg cgtccaccac catcgcttct ccggctttgc cgccccccaa gggttgcagg
    17101 tgtggacgca tagctcagtt ggtagagcag ctgactctta atcagcgggt cctaggttcg
    17161 agccctagtg cgtccaccat acctttctcc tgaaagacag ggtctgcttt tccccttttc
    17221 cgcagcgctt cgggttttcc gcagcgccag cgccatcgcc tcttgcagaa tggccgggaa
    17281 atgaaccaga aaggcacaat gacccaaagc ccccccgacg atcggccccc cgcggcagag
    17341 ccccctgcgg gcaccgacac ggccaccgct tccgacaagg tggccgatgc catcctgcgc
    17401 ggcatccgcg ccggcacctt tgccaccggc cagcatctgg tggagccgga tctgatgcgc
    17461 cggctgggga tcagccgcgg atcgctgcgc gaggcgctga aacggctggc cgccgagggc
    17521 atcgtcacgc tcaaccgctt tcgcggcgcc tatatctcct ccttcgaccg caagacggtg
    17581 ttcgacctac tgcagatgct ggagccgatg ttctgcctgg ccgcgcggct ggccgccgaa
    17641 cattgcgaca ccgcggaaaa gctggcccgc gtgcaggcgg tgaccgccag catcgaggcc
    17701 gccgccgccc gccgcaaccg cgccgactat atggagcagc ggcgcaattt ctattcggtg
    17761 ctgatcgagg tgggcggcaa cagcgaattg atgcgcgcca tgccgctggc ccgcaccgaa
    17821 ctgttccgcg cgcagatcga ggcggccctg tcggaacggc agcgccagct gcatgccggc
    17881 ggctatggcc ggatttcccg cgcggtgatc gagcgcaacc cggccgctgc cgaaagggcg
    17941 atgaagaagc acttcgccag cacccacaag acaatcagcg aattgcccga tcacgtgttc
    18001 gtggcggcca gctgaaccgc ctgatggcgg cgagccgggc actcaggcca tgtggccgcc
    18061 gccattggca tagagcacct gcccggtgat caccgccgcc tgcgccgagg ccaggaaggc
    18121 caccaccccg gcaatctcct ccacctcggc cagccggccc agcggcaggg cactggttac
    18181 cgcgcgggcg gcgccggtgg tatcgctggc catcagcgca tccaccgtct cggtgcgggt
    18241 ggggccggcg gccacggcat tcacccgcac ggcaggcgcc agttcggcgg ccaggctgcg
    18301 ggtgaagcct tccacccccg ccttgctggc gctgtaaacg gcaaggccgg gcgtggggaa
    18361 acgcgccgcg gtggacgaca tgttgacgat ggccccgccg cgcgcgcgca aggccggcgc
    18421 ggcggcccgg cagaaggcaa aggtggcgcc cagattggcc gccagcgtgg cggcgaattg
    18481 cgcatcgctg gtgtcttcca ctcgcgcggt gggatagctg ccggcgatat tggccagaat
    18541 gtcgagccc
//
LOCUS       DASRYE010000230         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_28340,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000230 DASRYE010000000
VERSION     DASRYE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28340"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFF98_11990"
     CDS             <1..1011
                     /locus_tag="VFF98_11990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZU64395.1"
                     /translation="PAPAPEPAPEPVAEPAPPPPPSPPPASPAPAPIAAGAHVLASPA
                     VRARARALGVDLAEVRAMAGGHIRHADLDAFLAYGAAGGFQPVGRRGVDEAVKVIGLR
                     RRIAERMAEAKRHIPHFSYVEECDVTLLEALRAQLNAPQGAPRGLAPAPERPHLTLLA
                     LFAAALCRALPDFPMMNARYDEEEGLLIRHGAVHLGVATQTAAGLMVPVLRHAEGLNL
                     WQLATGIARLAEAARSGRITRAELHGSTITVTSLGPLGGIAHTPIINRPEVAIIGPNR
                     IVERPMFVPDGLGGERIEKRKLMNISISCDHRVIDGQDAARFVQALRALLEAPARLLA
                     PA"
     gene            complement(1025..1732)
                     /locus_tag="VFF98_11995"
     CDS             complement(1025..1732)
                     /locus_tag="VFF98_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZU64396.1"
                     /translation="MSLPDWSLPDWEMPEWAEARGRALNHFPAIDPARAALVVIDMQG
                     AFITAGAPFANPRAEAIIPAVNRLAGRFRAARAPVIWTRQSVGPQPPLAMPAWQYDPA
                     DPFVAGAMAALTPGAALHGLHPAMAVAPGDAVIDKYRYSAFLCPAGALARELAARGSE
                     LAARGSELIVIVGTLTNICCESTARDGNMRGYKVVIAADATAAVTAEEERAALLNLRI
                     GFADVAGVAAIGARLEG"
     gene            complement(1729..2574)
                     /locus_tag="VFF98_12000"
     CDS             complement(1729..2574)
                     /locus_tag="VFF98_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZU64397.1"
                     /translation="MTNWLITGAGGGLGQDLAAAALARGDKVVATMRSAEAAAAFSAS
                     APGRALGLVLDLARPENAAAVVAEAESWLGQVDRLVNNAGFGMAGAIEETTLDQIRAL
                     FEVNVLGAIAMIQAVLPAMRAARAGHIINVTSMSGWAPWAGTAIYGASKYALECIGQT
                     LAQEVAELGIRVTNVAPGGMRTGFAGKGLVQAARHIADYDGAARFAQHSLAAGVGREK
                     GDPVRAAAAILAALDAPRPPVHLFLGEDSLRHIREHLDEVQAAMAEWEALTLSIAYPE
                     AEATA"
     gene            2782..>3220
                     /locus_tag="VFF98_12005"
     CDS             2782..>3220
                     /locus_tag="VFF98_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZU64398.1"
                     /translation="MQVDVVGSVNVDWVLRVADLPAPGETVMAAAATRLPGGKGANQA
                     AAAARMGVATRLLGAVGDDEAGQWMRGQLAAVGVDVAGLAAVPGWPTGAAHIAVDARG
                     ENQIIVVPGANGALGPVVPEAGVVLAQNEVPAASVASAFAAAGE"
BASE COUNT          441 a         1170 c         1156 g          453 t
ORIGIN      
        1 ccagcgcccg cgcccgaacc agcgcccgaa cctgtggcgg aacctgcgcc cccgccgccg
       61 ccatcgccgc cccctgcttc cccggcccct gcgcccatcg cggctggcgc acatgtgctg
      121 gcctcaccgg cggtgcgggc ccgggcccgg gcgctggggg tggatctggc cgaggtgcgg
      181 gcgatggctg gcggccatat ccgccatgcc gatctcgatg cgtttctcgc ttatggcgcg
      241 gcgggcggtt tccagccggt gggccggcgc ggggtggatg aggcagtgaa ggtgatcggc
      301 ctcaggcgcc gcattgccga gcggatggcc gaggccaagc gccacatccc ccatttctcc
      361 tatgtggagg aatgcgacgt gaccctgctg gaagccctgc gcgcccagct caacgcaccc
      421 cagggcgcgc ctcgggggct ggctcccgcg ccagaacgcc cgcatctgac gctgctggcg
      481 ctgttcgccg ccgccctgtg ccgcgccctg cccgatttcc cgatgatgaa cgcccgctat
      541 gacgaggagg agggcctgct catccgccat ggcgcggtgc atctgggagt ggccacgcaa
      601 accgcggccg ggctgatggt gccggtgctg cgccatgccg aagggctgaa cctgtggcag
      661 ctggccaccg gcatcgcccg tctggcagag gccgcccgca gcggccgcat cacccgggcc
      721 gaactgcatg gctccaccat caccgtcacc tcgctggggc cgctgggcgg cattgcccac
      781 acgcccatca tcaaccggcc ggaagtggcg atcatcggcc ccaaccgcat tgtcgagcgg
      841 ccgatgttcg tgcccgacgg gctgggcggc gaacgcatcg agaagcgcaa gctgatgaac
      901 atctcgatca gctgcgacca ccgggtgatc gacgggcagg atgccgcccg cttcgtgcag
      961 gccctgcgcg ccctgctgga agcgccggcg cggctgctgg cgccggcgtg aggggcaccc
     1021 cgtctcagcc ttccagccgc gcgccgatgg ccgccacccc ggccacatcg gcaaagccga
     1081 tccgcaggtt gaggagggcg gcgcgttcct cctcggcggt aaccgcggcg gtggcatcgg
     1141 cggcgatcac caccttatag ccccgcatgt tgccatcgcg cgcggtgctc tcgcagcaga
     1201 tgttggtgag cgtgccgaca atgacgatca gctcgctgcc gcgcgccgcc agctcgctgc
     1261 cgcgcgccgc cagctcgcgc gccaaagcgc cggccgggca gaggaaggcg ctgtagcggt
     1321 atttgtcgat cactgcatcg cccggcgcca ccgccatggc cgggtgcagc ccatgcagcg
     1381 ccgcgcccgg cgtcagcgcg gccatggcgc cggccacgaa gggatcggcc gggtcatact
     1441 gccaggcggg catggccagc ggcggctggg ggcccacgct ctgccgggtc cagatcaccg
     1501 gggcgcgggc ggcgcgaaac cgcccggcca gccggttcac cgccggaatg atcgcttctg
     1561 cccgcggatt ggcaaaaggc gcgccggcgg tgatgaaggc cccctgcatg tcgatcacca
     1621 ccagcgccgc gcgggccgga tcgatcgccg ggaaatggtt gagcgcccgc ccgcgcgcct
     1681 ccgcccattc cggcatctcc caatccggca ggctccaatc cggcaggctc atgccgtggc
     1741 ttcggcctcg ggataggcga tggaaagcgt cagcgcttcc cattcggcca tggcggcctg
     1801 cacctcgtcc agatgctcgc ggatgtggcg cagcgaatcc tcgcccagga acaggtgcac
     1861 cggcgggcgc ggcgcatcca gcgcggccag aatggcggcg gccgcccgca ccggatcgcc
     1921 cttttcgcgg ccaacgccgg cggcaaggct gtgctgggca aagcgcgcgg cgccatcgta
     1981 atcggcgatg tggcgggccg cctgcaccag ccccttgccg gcaaagcccg tgcgcatgcc
     2041 gcccggcgcc acattggtca cgcggatgcc cagctccgcc acttcctgcg ccagcgtctg
     2101 gccgatgcat tccagcgcat atttgctggc gccatagatc gccgtgcccg cccacggcgc
     2161 ccagccgctc atcgaggtga cgttgatgat atggccggcg cgcgccgcgc gcatggccgg
     2221 cagcaccgcc tggatcatgg cgatggcgcc cagcacgttc acttcgaaca gcgcgcggat
     2281 ctggtccagc gtggtctctt caatggcgcc tgccatgccg aagccggcat tgttcaccag
     2341 ccggtccacc tggcccagcc agctttccgc ctcggccacc acggcggcgg cattctcggg
     2401 ccgggccaga tccagcacaa ggcccagcgc gcggccgggc gcgctggcgc tgaaggccgc
     2461 tgccgcctcg gcgctgcgca tggtggccac caccttgtcg ccccgcgcca gcgcggccgc
     2521 cgccagatcc tgccccagcc cgccgccggc gccggtgatc aaccagtttg tcatgccttc
     2581 ctccacacct ttggcctgcg ctgcggcgcc aggctccatg caacacacaa gggggcaaca
     2641 tagggcggtg gcggccgcgc tcttgccgca ggcggcccca aatcttgggg cagggcgcat
     2701 ggccgccggg caccgcttgc cagcggcgcg cgcgcgtggt tccagcaagg ccggcttgat
     2761 gcaccggaaa atgagggcag gatgcaggtg gacgtggtgg gctctgtgaa tgtggactgg
     2821 gtgctgaggg tggccgatct gccggccccg ggcgaaacgg tgatggcggc cgcggccacg
     2881 cgcctgcccg gtggcaaggg ggccaatcag gcggcagcgg ctgcccgcat gggcgtggcc
     2941 acccgcctgc tgggcgcggt gggcgatgat gaggcgggcc aatggatgcg cggccagctg
     3001 gcggcggtgg gggtggatgt ggccgggctg gcggccgtgc caggctggcc cacgggggct
     3061 gcgcatattg ccgtggatgc gcgcggcgag aaccagatca tcgttgtgcc cggggccaat
     3121 ggcgcgctgg ggccggtggt gcccgaggcg ggcgtggtgc tggcgcagaa cgaggtgccc
     3181 gccgccagcg ttgccagcgc cttcgccgcg gccggcgagg
//
LOCUS       DASRYE010000231         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_43203,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000231 DASRYE010000000
VERSION     DASRYE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43203"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFF98_12010"
     CDS             <1..570
                     /locus_tag="VFF98_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HZU64399.1"
                     /translation="DPRSEAPAMPRPEPVAAPVAQPPASDPATEAEARGHARGLAEGL
                     ARARAEAEADRAAQAALQLAFAHLDTQLAEQLRQRLTDTVVALCEHALAPLAVDEAAL
                     ALRVRRAVAMLARADDERVICLHPADLTLVRAALPADWTFRPDPRLERGALRVETQGG
                     GVEDGPREWRRAIAEALDIGGPEALEDEA"
     gene            606..1904
                     /locus_tag="VFF98_12015"
     CDS             606..1904
                     /locus_tag="VFF98_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HZU64400.1"
                     /translation="MAGAQVDITPRRYGVVASCDGGLLEISGLAVPVGALCRIPQGSG
                     RGLAAEVIGFRNGRTLAMLLGDMVNLRPGARVRPEGQPGMLPVGEAFLGRAVDGEGHP
                     IDGLGPLHPRALWPAGGVRAGALDRSPVRLPFDTGVRALNAITTFGVGQRIGIMAGSG
                     VGKSVLLDMIAHGASAEIVIVGLIGERAREVSDFVERHMAGTKRATTAVIAVPADHAP
                     NLRLRGALLATAMAEYFRSQGRRVLLIMDSLTRVAHAGREIGLLLGEPGAARGYPPSA
                     LATITKLVERAGNSAASGGSITGLYTVLADGDNQDDPVVDTARSILDGHIVLSRELAQ
                     RGQYPAIDIAASLSRVMSDIVSPGHQRAARAFRALVASYEANRDLVLMGAYRAGADPQ
                     LDQAIAMHPALVAFLAQEGGRIVPLAASVAELLQLTGMQG"
     gene            1910..2335
                     /locus_tag="VFF98_12020"
     CDS             1910..2335
                     /locus_tag="VFF98_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64401.1"
                     /translation="MQAEKRKLARLHRLERLRAIAKQAAASEAARAEATLSQLALLAE
                     RTGQLAADYAARTDAEDGAALGRIGRFAGSLQGLRAATEADVSRARAIADERQSQLAT
                     AERRRAAVEDRAEAQARQLAARRHYAVLAGRRGFGTKLD"
     gene            2382..>3574
                     /locus_tag="VFF98_12025"
     CDS             2382..>3574
                     /locus_tag="VFF98_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64402.1"
                     /translation="MPDCAASVSLAALAPSQIATLAALPAALPATGADAGPQSDFGSV
                     LAGQTAAPAQSAQTGQTTIVQGAVQAADQPAQASAPPQTALNLPAAAPAPLAASTLAP
                     MAPTALAATLAALPQALVSAGQQPAPATATASPPTAQDTPATPAPAASATSAPPITPA
                     PEDPAAANAADTIALAAGTSLPLAGNSLPALPQPLTSTASSAPQAKAPRQPSDSDDDS
                     DGASSAATAAPATDTPVLAALMGLAPLVPATPAPSPQPGNESQTPAPSAAPVTPTASI
                     QPVAQAGSQPAAPAAAPLQALAASPALPASNGSSTNSASGSAAGSTPATPAAAPTVLA
                     MALPAVSLPVSALPAATLPVSAALPAMAAPLVTGTAARTLALPTVSARLAAAPLAVNS
                     VASPAA"
BASE COUNT          480 a         1423 c         1221 g          450 t
ORIGIN      
        1 gacccccgca gcgaggcgcc tgccatgccg cggccagaac ccgtggcagc ccccgtggcc
       61 cagccccccg ccagcgatcc tgccacagag gccgaggcgc gcggccatgc ccgcggcctg
      121 gccgaagggc tggcccgcgc ccgcgccgaa gccgaggccg atcgcgccgc ccaggccgcc
      181 ctgcagctgg ctttcgctca cctcgacacc cagctggccg agcaattgcg ccagcgcctc
      241 accgacacgg tggtggccct gtgcgaacat gcgctggcgc cgctggcggt ggatgaggcg
      301 gcgctagcgc tgcgggtgcg gcgggcggtg gccatgctgg cgcgcgccga cgacgagcgg
      361 gtgatctgcc tccaccccgc cgacctcacc ctggtgcgcg ccgccctgcc ggccgactgg
      421 accttccgcc ccgatccccg gctggaacgc ggcgcgctgc gagtggaaac gcaaggcggc
      481 ggcgtggagg atggcccgcg cgaatggcgc cgcgccatcg ccgaggcgct cgatatcggc
      541 gggcccgagg cgcttgagga cgaggcgtga gcctgccccc atcgctggac agcctgctgg
      601 ccgacatggc cggcgcgcaa gtggatatca ccccgcgccg ctatggcgtg gtggcctcct
      661 gcgatggcgg gctgctggaa atttccggcc ttgccgtgcc ggtgggcgcc ctgtgccgca
      721 ttccgcaagg gagcgggcgg gggctggcgg ccgaagtgat cggctttcgc aatggccgca
      781 cgctggccat gctgctgggc gacatggtga atttgcgccc cggcgccagg gtgcggccgg
      841 aaggccagcc gggcatgctg ccggtgggcg aggcgttcct gggccgcgcg gtcgatggcg
      901 aggggcaccc gatcgacggg ctgggcccgc tgcacccccg cgcgctgtgg ccagccggcg
      961 gcgtgcgtgc cggcgcgctt gatcgcagcc cggtgcgcct gcctttcgac accggcgtgc
     1021 gcgcgctcaa cgccatcacc acctttggcg tgggccagcg cattggcatc atggcgggtt
     1081 ctggcgtggg taaatcggtg ctgctcgaca tgatcgccca tggcgccagc gccgagatcg
     1141 tcatcgtcgg cctgatcggc gagcgcgcgc gcgaagtgtc tgattttgtg gagcggcaca
     1201 tggccggcac caagcgcgcc accaccgctg tcatcgccgt gccggcagac catgccccca
     1261 acctgcgcct gcgcggcgcc ctgctggcca ccgccatggc cgaatatttc cgcagccagg
     1321 gccggcgcgt gctgctgatc atggacagcc tgacccgcgt ggcccacgcc ggccgcgaga
     1381 tcggcctgtt gctgggcgag ccgggggcag cgcgcggcta tcccccttcg gcgctggcca
     1441 ccattaccaa gctggtggag cgggcgggca attcggccgc ctcgggcggc tcgatcaccg
     1501 ggctctacac cgtgctggcc gatggcgaca accaggacga tccggtggtg gacaccgccc
     1561 gctcgatcct cgacgggcat atcgtgctga gccgcgagct ggcccagcgc gggcaatatc
     1621 ccgccatcga catcgccgcc tcgctcagcc gggtgatgag cgacatcgtc agccccggcc
     1681 accagcgcgc cgcccgcgcc tttcgcgcgc tggtggccag ctatgaggcc aaccgcgatc
     1741 tggtgctgat gggcgcctac cgcgccgggg ccgatccgca gctggatcag gccatcgcca
     1801 tgcatcccgc gctggtggcc tttctcgcgc aggagggcgg caggatcgtg ccgctggcgg
     1861 caagtgttgc cgagctcctg cagctgaccg gcatgcaagg ctaggccccg tgcaggcgga
     1921 aaaacgcaag ctggcccggc tgcaccggct ggaacggctg cgcgccattg ccaagcaggc
     1981 cgccgccagc gaagccgccc gcgccgaggc cacgctgagc cagctggccc tgctggccga
     2041 gcgcaccggc cagctggccg ccgattatgc cgcccgcacc gatgccgagg atggcgccgc
     2101 gctggggcgg atcggccggt ttgccggcag tttgcagggc ctgcgggctg ccaccgaggc
     2161 cgatgtgagc cgcgcccgcg ccattgccga cgaacgccag agccagctgg ccaccgccga
     2221 acgccgccgc gccgccgtgg aagaccgcgc cgaggcacag gcccggcagc tggccgcgcg
     2281 ccgccattac gctgttctgg caggccggcg cggatttggc acgaaacttg attgatcgtc
     2341 agggctctcc tcgcggaggg cgctttgcca aggatctgcc gatgccggat tgcgccgcct
     2401 ctgtctccct cgctgccctg gcccccagcc agatcgccac gctggctgcc ctgccggccg
     2461 ccctgccggc cacgggcgcc gatgccggcc cgcagagcga tttcggcagc gtgctggccg
     2521 ggcagacggc agcgccggcc cagagcgcgc aaactggcca gacaacgatc gttcagggag
     2581 ccgttcaggc cgccgatcag cccgcgcagg ccagcgcccc gccgcaaacc gcgctcaacc
     2641 tgccggccgc agcgcccgcg ccattggcgg cgtccactct ggcaccaatg gcgcccactg
     2701 cccttgccgc cacgctggcc gccctgccgc aagccctggt ttccgccggc cagcagcccg
     2761 cgccggcaac cgccaccgcc agcccgccca ccgcgcaaga caccccggcc acgccagcgc
     2821 cagcggcttc agccacatcg gcgcccccga tcacgccggc ccctgaagat ccggccgctg
     2881 ccaacgcggc agacacgatc gcccttgctg ccggcacatc cttgccgctt gccggcaaca
     2941 gcctgccggc cctgccccag ccgctgacca gcaccgccag cagcgccccg caggccaaag
     3001 ccccccgcca gccatccgac agcgatgacg acagcgacgg ggccagcagc gctgccaccg
     3061 ccgcccctgc taccgacacc ccggtgctgg ccgcgctgat gggcctggcc ccgctggtgc
     3121 cggcaacgcc cgcgcccagc ccgcagcccg gcaacgagag ccagaccccg gccccaagcg
     3181 ctgcgccagt aacaccaacc gccagcatcc agccggtggc acaagccggc agccagcctg
     3241 cggcccccgc cgccgcgccc cttcaggccc ttgccgcaag ccccgccttg cccgccagca
     3301 acggttccag caccaacagc gcttcgggca gcgctgcggg cagcaccccc gccacgccgg
     3361 cagcggcgcc cacggtgctg gccatggccc tgccggccgt ttccctgccc gtttcagccc
     3421 tgcccgccgc caccctgcct gtgtctgctg ccctgcccgc catggccgcg ccgctggtca
     3481 cgggcacggc cgcccggacg ctggccctgc ccaccgtttc ggccagactg gccgccgcgc
     3541 cgctggcggt gaacagcgtc gccagccccg cggc
//
LOCUS       DASRYE010000232         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_225293,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000232 DASRYE010000000
VERSION     DASRYE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225293"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..2400)
                     /locus_tag="VFF98_12030"
     CDS             complement(100..2400)
                     /locus_tag="VFF98_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64403.1"
                     /translation="MRMNPNYALRLGVAFCALGVALPVLAAPALADEAAQPKSMGDGE
                     IVVTAQRRAEKVTKVPISITVADAGQLERQQVNTVNDLNRIAPSLEIQSAPGQNTGGG
                     GAIRGIGTQTFSAGAVASVGVVVDQVSQGNANISDLFDVARVEVLKGPQGTLFGLTTS
                     AGVINITTNKPDFNKFSARVRTELSGAGTAGSKYGDQVVQGVVNVPLASNAAVRVAGF
                     TNLRQGPDYDVTTGKYVNNDTWGLRGHLLWEPTSRLTVNIIGDYNKTRTTNGGDFFTF
                     VKTAGAGSSNIASGFAVADNMTGNLASCGVTAAVGNRSFCTSQNYTDHLYSGGVSLQV
                     DYQADPFTITSITAYRKSLESGYGAATNVLRGDSLLLDIANAAVHRPTSLITQELRIS
                     SPAGGLVEYTGGLFISSQIAHRGAEPLAVYAQVAPGYYIDVHDGGAGESRVSDNSQAI
                     FGQATIHATPKLRLIVGGRYTSDQLKLDTVADAVAYSTTGHTGLTVNKFSWKLGAQYD
                     VARRTMVYGTVTRGFKGGQIAQPTDAAAYVVQPEVPTSYELGLKSTLLGGWILDTDLF
                     YQTIKNFQAQTCTVDTSNVINCLQQNINGVKSRGAEINLFGKVTHDLSLNTGFIWAKA
                     TYPKGYIGTDGNDIGGTQLAYAPEYKFTLSGEYSKAITRTYNGFLALDTVWKSRVRYE
                     ANTLADTTYHAHWTVGGRIGVRTADDRFTVALFARNLFNEHEPALMQSSLPYTTSVGG
                     VTQQNIGAIYGPQSFRQIGLSLDGKF"
     gene            complement(2520..3350)
                     /locus_tag="VFF98_12035"
     CDS             complement(2520..3350)
                     /locus_tag="VFF98_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU64404.1"
                     /translation="MRYVSFTRPDGSASFGRLAGETVFDLGHAGHADLKAAIAADALA
                     DLADGTAFALGDITLLPVIPNPEKILCVGLNYATHVKETGREQKEHPAIFHRWANSLV
                     AEGAALVCPPETTRFDYEGELAIVIGKAGRRIKAQDAWDHIAGYAPFNDGSVRDWQRH
                     NIQFLPGKTWPGTGGFGPALVTPDEVADLGSQRVQTRLNGELVQDQPIADMIWDIPTV
                     IAYCSTFTELQPGDVIATGTPGGVGDKRNPPLYMKGGDIVEISVGVIGTLRNPVVDEA
                     "
     gene            complement(3357..4289)
                     /locus_tag="VFF98_12040"
     CDS             complement(3357..4289)
                     /locus_tag="VFF98_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64405.1"
                     /translation="MKLRSIELALPKAAEAHRWMVDIWGCADAGHGVAGGRDTFYLRG
                     SGSFPYLVALSESAENFVRSTTFVCSHERLAQVAQAAAARGLNAAPCTSEDPGGGHGI
                     VVELAEGELLRFLVDAQEVAPIAPHAKATRRVMPVKLTHVVFNAADAEASGDLVEAVL
                     GFRVSDRTKGMVFVRCNEAHHSTAFARAGFSSLNHIAFEMADLDAVMRGIGNMRDAGF
                     APAWGPGRHGPGDNVYAYYIAPFGPVIEYSTAVEKVAEDYQPGAPEDWTWPDRRIDQW
                     GVSDKDFAGLRVAEEAFRFRREWHPAPTDQLAGA"
     gene            complement(4297..4716)
                     /locus_tag="VFF98_12045"
     CDS             complement(4297..4716)
                     /locus_tag="VFF98_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZU64406.1"
                     /translation="MTISLSAAQAILTAGLGHARAAGLKPVAVVVLDAGGHPVAMARE
                     DGATFFRAEIARAKAYGALGMGANTAVIAERARGNPTFFGGVVTTVGGDLVFSPGGVL
                     VMAGGVVVGAVGVSGDTGENDEAVAQAGIAAAGLAKE"
     gene            complement(4713..>5496)
                     /locus_tag="VFF98_12050"
     CDS             complement(4713..>5496)
                     /locus_tag="VFF98_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64407.1"
                     /translation="DAAMMVPAKVAELLAPWVGDVPHTIVRSATYRFHGLVAEQWQVG
                     QVFLAGDAAHQTPPFFGQGMCHGLRDVANLAWKMAAIVQEGAHPDILATYQPERDPHV
                     RAVIGAAVAAGRYICELDPAKAAARDAEMVARAAAQAGQRQTAHDLIPPIATGLLATG
                     TPGAGERFIQPVLADGRKLDEATGDGWRLFAHGAPCVPAGVAAVALETLADAGAVAGW
                     LADRGGEAVLVRPDHYVFGTGHPAALIAARAAALGLAQEELA"
BASE COUNT          903 a         1809 c         1864 g          920 t
ORIGIN      
        1 cggcactggc agcggcagcg ttatcctgca tccctggcat tcctgtgtaa ggccttggca
       61 aaaagggcgg ggcttgcagc cccgccctct ttttctagat cagaacttgc cgtcgagcga
      121 caggccgatc tggcggaagg attgcggccc atagatggcg ccgatgttct gctgcgtcac
      181 cccgcccacg ctggtggtat agggcagcga gctttgcatc agggccggct cgtgctcgtt
      241 gaacaggttg cgggcaaaga gcgccacggt gaagcgatca tcggccgtgc gcacgccgat
      301 gcggccgccc actgtccaat gggcatgata ggtggtgtcg gccagcgtgt tggcttcata
      361 gcgcacgcgc gacttccaca ccgtgtcgag cgcgaggaag ccgttgtagg tgcgggtgat
      421 cgccttgctg tattcgcccg agagggtgaa cttgtattcc ggcgcatagg ccagctgggt
      481 gccgccgata tcattgccat cggtgccgat atagcccttg ggataggtgg ccttggccca
      541 gatgaagccg gtgttcagcg acagatcatg cgtgaccttg ccgaacaggt tgatctcggc
      601 cccgcgcgac ttcacgccgt tgatgttctg ctgcaggcag ttgatcacat tgctggtgtc
      661 aacggtgcag gtttgcgcct ggaagttctt gatcgtctgg tagaacaggt cggtatcgag
      721 gatccagccg cccagcagcg tgctcttcag gcccagttcg taggaggtgg gcacttcggg
      781 ctgcacgaca taggcggcgg catccgtcgg ctgggcgatc tgcccgccct tgaagccgcg
      841 cgtcaccgtg ccatagacca tcgtgcggcg ggccacatca tattgcgcgc ccagcttcca
      901 cgagaacttg ttgacggtaa ggccggtgtg gccggtggtc gaataggcca ccgcatcggc
      961 caccgtgtca agcttcagct ggtcgctggt gtagcgcccg ccaacgatca ggcgcagctt
     1021 gggggtggca tggatggtgg cctggccgaa gatcgcctgc gaattgtcgg acacgcgact
     1081 ttcgccggcg ccgccatcat gcacgtcgat gtagtagccg ggcgccacct gggcatagac
     1141 ggccagcggc tctgccccgc gatgggcgat ctggctggag atgaacaggc cgccggtata
     1201 ttccaccagc ccgccagccg gcgaggaaat gcgcagttcc tgggtgatca ggctggtcgg
     1261 ccggtgcacc gcggcattgg cgatatcgag cagcagcgaa tcgccgcgca gcacgttggt
     1321 ggccgcgcca tagcccgatt ccaggctctt gcgataagcg gtgatcgagg tgatggtgaa
     1381 cggatcggcc tgataatcca cctgcagcga aacgccgccg ctgtagaggt ggtcggtgta
     1441 gttctggctg gtgcagaagc tgcggttacc cacagcggcc gtcacgccgc agctggccag
     1501 gttgccggtc atgttgtcgg ccacggcaaa gcccgaggcg atgttcgagg agccggcgcc
     1561 ggcggtcttc acgaaggtga agaagtcgcc gccattggtg gtgcgggtct tgttatagtc
     1621 gccgatgatg ttgacggtga ggcgcgaggt cggctcccac agcaggtggc cgcgcaggcc
     1681 ccaagtgtcg ttgttgacat atttgcccgt cgtcacgtca taatccgggc cctggcgcag
     1741 gttggtgaaa ccggccacgc gcaccgccgc attgctggcc agcggcacgt tgaccacgcc
     1801 ctgcaccacc tgatcgccat atttggaacc agccgtgccc gcgcccgaca gttcggtgcg
     1861 cacgcgcgcg gagaacttgt tgaaatcagg cttgttggtg gtgatgttga tgacaccggc
     1921 cgaggtcgtc aggccgaaca gcgtgccttg cgggcctttc agcacttcca cgcgggccac
     1981 atcgaacagg tcggagatat tggcattgcc ctggctgacc tgatcaacca ccacgccgac
     2041 agaggccacg gcgcccgccg agaaggtctg cgtgccgatg ccgcggatgg cgccgccgcc
     2101 gccggtgttc tggcccgggg cagactggat ctcgaggctg ggggcgatgc ggttgaggtc
     2161 gttcaccgtg ttgacctgct ggcgctccag ctggccggca tcggccacgg tgatcgagat
     2221 cggcactttc gtcaccttct cggcgcggcg ctgggcggtc accacgatct cgccatcgcc
     2281 catgctcttg ggctgggcgg cttcatcggc cagggcgggc gcggccagca ccggcagggc
     2341 cacccccagc gcgcagaagg caacgccaag gcgaagggca taattcgggt tcatacgcat
     2401 cgttcatctc cccaaaggct ggcccggcgg ggtgcgcgcc ccgccggtgc tgttctgttc
     2461 aatgtgcggg ccttgtggcc ttttcgcagt cagtggtatt tggttgcggg cagcgggcct
     2521 caggcctcgt ccaccaccgg gttgcggagc gtgccgatca cgcccacgga aatttccaca
     2581 atgtcgccgc ccttcatgta gagcggcgga ttgcgcttat cgcccacgcc gccgggcgtg
     2641 ccggtggcga tcacatcgcc cggctgcagc tcggtgaagg tggagcaata ggcgatgacg
     2701 gtggggatat cccagatcat gtcggcgatc ggctgatcct gcaccagctc gccgttgagg
     2761 cgggtctgca cgcgctggct gcccagatcc gccacctcgt cgggggtgac aagcgccggg
     2821 ccgaagccgc cggtgccggg ccaggtcttg ccgggcagga actggatgtt gtggcgctgc
     2881 cagtcgcgca cggagccatc gttgaagggg gcatagccgg cgatatggtc ccaggcatct
     2941 tgcgccttga tgcgccggcc cgccttgccg ataacgatgg ccagctcgcc ctcataatcg
     3001 aagcgggtgg tttcgggcgg gcacaccaga gcggcgcctt ctgccaccag cgaattggcc
     3061 cagcgatgga agatcgccgg gtgttccttc tgctcgcggc cggtttcctt cacatgggtg
     3121 gcatagttca gccccacgca gaggatcttt tccggattgg gaatgaccgg cagcagggtg
     3181 atatcaccca gcgcgaaggc cgtgccatcg gcgagatccg ccagcgcatc ggcggcgatc
     3241 gccgccttca gatcggcatg gccggcatgg cccagatcga acaccgtctc gccggcaagg
     3301 cggccgaagg aggcgctgcc atcggggcgg gtgaaagaga cgtaacgcat ggggggtcag
     3361 gctcctgcaa gctggtctgt gggggcgggg tgccattcgc ggcggaagcg gaaggcttct
     3421 tcggccacgc gcaggccggc aaaatccttg tccgacacgc cccactggtc gatccgcctg
     3481 tcgggccagg tccagtcctc gggggcgccg ggctgataat cctcggccac cttctcaacg
     3541 gcggtggaat attcgatcac cgggccgaag ggcgcgatgt aataggcata gacattatcg
     3601 cccgggccat ggcggccggg gccccaggca ggcgcaaagc cggcatcgcg catgttgcca
     3661 atgccgcgca tcaccgcatc aaggtctgcc atctcgaagg cgatgtggtt gagcgaggag
     3721 aagccggcgc gggcaaaggc ggtggaatga tgcgcctcgt tgcagcgcac gaagaccatg
     3781 cccttggtgc ggtccgacac gcgaaagccc agcaccgctt ccaccagatc gccgctggcc
     3841 tcggcatcgg cggcgttgaa caccacatgg gtcagcttca ccggcatcac ccggcgggtg
     3901 gccttggcgt ggggggcgat cggcgccacc tcctgcgcat cgacaaggaa gcgcagcagc
     3961 tcgccctcgg ccagttcgac gacgatgcca tggccgccgc ccggatcctc gctggtgcag
     4021 ggcgccgcgt tgaggccgcg cgcggcggcc gcctgcgcca cttgcgccag ccgctcatgg
     4081 ctgcacacga aagtggtgga acgcacgaaa ttctccgcgc tttccgacag cgccaccaga
     4141 taggggaagc tgcccgaacc gcgcaggtag aaggtgtcgc gcccgcccgc cacgccatgg
     4201 ccggcatcgg cgcaccccca gatatccacc atccagcgat gggcctcggc cgccttgggc
     4261 agggccagtt cgatgctgcg cagcttcatc atcggcttat tcctttgcca ggccggccgc
     4321 ggcaatgccc gcctgcgcca ctgcttcgtc attctcgccg gtgtcgcccg agacgcccac
     4381 cgcgcccacc accacgccgc cggccatcac cagcacgccg ccgggcgaaa acaccagatc
     4441 gccgcccacc gtggtgacaa cgccgccgaa gaaggtgggg ttgcccctgg cgcgctcggc
     4501 gatcacggcg gtgttggccc ccatgcccag cgcgccatag gccttggccc gggcgatctc
     4561 ggcgcggaag aaggtggcgc catcctcgcg ggccatggcc accggatggc cgccggcatc
     4621 cagcaccacc acggccacag gcttcagccc ggccgcgcgg gcatggccaa ggccggcggt
     4681 caggatcgcc tgtgcggcgg aaagggagat cgtcatgcca gttcttcctg agccaggcca
     4741 agcgctgcgg cccgcgcggc aatcagcgcg gccggatggc ccgtgccgaa cacgtaatga
     4801 tcgggccgca ccagcaccgc ctcgcccccc ctgtcggcca gccagccggc aacagcgccg
     4861 gcatccgcca gcgtttccag cgccacggcg gcaacgcctg ccggcacaca gggcgcgcca
     4921 tgggcaaaca agcgccaacc atcgccggtg gcctcgtcca gcttgcggcc atcggccagc
     4981 accggctgga tgaagcgctc gccagcgccg ggcgtgccgg tcgccagcag gccggtggcg
     5041 atcggcggga tcagatcatg cgcggtctgg cgctggccgg cctgggcggc ggcgcgggcc
     5101 accatctcgg catcgcgggc cgctgccttg gccggatcca gctcgcagat atagcgcccg
     5161 gccgccactg ccgcgccgat caccgcgcgc acatgcggat cacgctcggg ctgataggtg
     5221 gcgaggatat cgggatgggc gccctcctgc acgatggcgg ccatcttcca ggccagattg
     5281 gcgacatcgc gcaggccatg gcacatgccc tggccgaaga agggcggcgt ttgatgcgcg
     5341 gcatcgccag ccagaaacac ctggcccacc tgccactgct ctgccaccag cccgtggaag
     5401 cgataggtgg ccgagcgcac gatcgtgtgc ggcacatcgc ccacccaggg cgccagcagt
     5461 tcggccacct tggcaggcac catcatcgcg gcatcc
//
LOCUS       DASRYE010000233         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_61811,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000233 DASRYE010000000
VERSION     DASRYE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61811"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFF98_12055"
     CDS             complement(<1..441)
                     /locus_tag="VFF98_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZU64408.1"
                     /translation="MQGETIFALSSGLPPAGVAVIRVSGPGAGPALAGLARAMPPARQ
                     ASLRVLRDRAGDVLDQALVLWLPGPRTVTGEDMAEIHCHGGRAVVAALLAALGAMDGL
                     RMAEAGEFTRRAFANGRIDLAEAEGLADLLSAETELQRRASLAMA"
     gene            complement(474..713)
                     /locus_tag="VFF98_12060"
     CDS             complement(474..713)
                     /locus_tag="VFF98_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HZU64409.1"
                     /translation="MKVNVEVECTPEEARRFLGLPDVTAANQAYMDAVLNAMKGMGSL
                     EQMGEFVKQLAPMGQFGLKMFQQFMETSTGLAGRK"
     gene            848..1546
                     /locus_tag="VFF98_12065"
     CDS             848..1546
                     /locus_tag="VFF98_12065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU64410.1"
                     /translation="MATNTLIPTLDHSGEIPAYVARPAGTPRGAIIVVQEIFGVNSGI
                     VKKAEDWAAEGYLAIAPDIFWRQEAGVELDHDIPAEFQKAIQLMLAHDFDRGVEDLKA
                     VLAWARDEGTPRVGLVGFCMGGKIAYQMAARSTIDAAVGYYGVGLDQMLDEAANITGK
                     LLLHIPTADEFVTAAAQAAVHQGLEGNPHITLYDYEGLGHGFAETHGSRRHEAGAQLA
                     DERSRAFLAEVLTA"
     gene            complement(1677..2933)
                     /gene="rho"
                     /locus_tag="VFF98_12070"
     CDS             complement(1677..2933)
                     /gene="rho"
                     /locus_tag="VFF98_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZU64411.1"
                     /translation="MHLKELKKKTSAELVEMAEELGVEGASTLRRQDLMFAVLKELAE
                     DGEEILGIGTIEVLPDGFGFLRSPEANYLAGPDDIYVSPNQVRKWGLRTGDTVEGEIR
                     APKDGERYFAITKLIKVNFDDPEAVRHRVNFDNLTPLYPNQRLKLDTLDPTVKDKSAR
                     VIDLISPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTD
                     MQRSVKGEVISSTFDEPAARHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVPKDQLSKMWVLRRILMQMGT
                     VDAMEFLLDKMKDSKTNEDFFATMNQ"
     gene            complement(3109..3552)
                     /locus_tag="VFF98_12075"
     CDS             complement(3109..3552)
                     /locus_tag="VFF98_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZU64412.1"
                     /translation="MDWIISMTYLWLKAGHIIFVIFWMAGLFMLPRYFVYHQEAEAGS
                     AEEARWVEREQKLLKIILWPSLVAVWLLGLALAHAIGAFAHGWFHLKLAVALGLSFYH
                     IWLARYAGALARGERRLSGRHLRMLNEVPGLAAAVIVVMVVVKPF"
     gene            complement(3565..4590)
                     /gene="hemE"
                     /locus_tag="VFF98_12080"
     CDS             complement(3565..4590)
                     /gene="hemE"
                     /locus_tag="VFF98_12080"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZU64413.1"
                     /translation="MPGLLIDTLRGANAKQRPIWLMRQAGRYLPEYRALRAEKGGFLA
                     LVYDCAAAAEITLQPLRRFGFDGAILFSDILIVPYAMGQDLQFLVGEGPHLAPRLVDH
                     ALNGLQSVPERLLPIYETVRQVVAQLSPEQTMLGFAGSPWTIATYMVNGEGSRDQHET
                     RAYAYRDRAAFQAIIDAIVGVTVEYLLGQIEAGAEAVQLFDSWAGSLAPAEFERWVIA
                     PNAAIVAGVKARAPHVPIIGFPKGAGEKLAAYARETGVDALGIDETIDPVWAARTLPA
                     GMPVQGNLDPLLLLAGGAEMEARALGVLEAFADRPHVFNLGHGIGQTTPIAHVEQLLA
                     IVRGWSR"
     gene            4913..>5236
                     /locus_tag="VFF98_12085"
     CDS             4913..>5236
                     /locus_tag="VFF98_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HZU64414.1"
                     /translation="MTRLHLHLLSDSTGETLEMIAKAGLAQFDDADVVRHFWPMVRSQ
                     QHLDRIMGDIAAHPGLVLFTIVSADIRTLLEERCRAMGLPAVPAIDAVIDALENALGQ
                     ESKGRP"
BASE COUNT          971 a         1806 c         1530 g          929 t
ORIGIN      
        1 cgccatcgcc aggctggccc gccgctgcag ctccgtctcg gccgagagca gatcggccag
       61 cccttccgct tcggccagat cgatgcggcc attggcaaag gcgcggcgcg tgaactctcc
      121 cgcctcggcc atgcgcaaac catccatcgc gcccaatgcc gccagcagcg ccgccaccac
      181 cgcccgcccg ccgtggcagt ggatctcggc catatcctcg cccgtcaccg tgcgcgggcc
      241 gggcagccac aacaccagcg cctgatccag cacatcgccc gcccgatcac gcagcacccg
      301 caagctggcc tgccgcgctg gcggcatggc ccgcgccagc cccgccaacg ccggcccagc
      361 ccccgggccg cttacccgga tcaccgccac acccgccggc ggcaatccgc tcgacagtgc
      421 aaaaatcgtt tcgccctgca tgtctggtca aaatgtctcg tctaagtggc ggcctatttg
      481 cggccagcca accccgtgct ggtttccatg aactgctgga acatcttcag gccaaactgg
      541 cccatcggcg ccaactgctt cacaaactcg cccatctgct ccaggctgcc catgcccttc
      601 atcgcattca gcaccgcatc catataggct tggttcgccg ccgtcacatc cggcaggccc
      661 agaaaacgcc gcgcctcttc gggcgtgcat tccacttcca cattcacttt cattgtcgcg
      721 tttctcctgc cctgccgcct tgcgggtccc tcgcctgccc gcaaggctga cacatgccct
      781 agccaaagtc catctagggg ctttaggaag gattgtgctc ccaaccattc cccccaggag
      841 atatcccatg gccaccaaca cacttatccc cacccttgac cacagcggcg agatccccgc
      901 ctatgttgcc cgcccggccg gcactccgcg cggcgccatc atcgtggtgc aggaaatttt
      961 cggcgtgaac tccggcatcg tgaagaaggc cgaggattgg gcggcggaag gctatcttgc
     1021 catcgccccc gatatcttct ggcggcagga agccggggtt gagctggacc acgatattcc
     1081 cgcagaattc cagaaggcca tccagctgat gctggcccat gatttcgatc gcggcgtgga
     1141 agatctgaag gcggtgctgg cctgggcccg cgatgaaggc accccgcgcg tggggctggt
     1201 gggcttctgc atgggcggca agatcgccta tcagatggcc gcccgcagca ccatcgatgc
     1261 cgccgttggc tattatggcg tcgggctgga ccagatgctg gacgaggccg ccaacatcac
     1321 cggcaagctg ctgctgcaca tccccaccgc cgatgaattc gtgaccgccg ccgcccaggc
     1381 cgccgtgcac caggggctgg aaggcaaccc gcacatcacc ctctacgatt acgaagggct
     1441 gggccatggc tttgccgaaa cccacggcag ccgccgccac gaagccggcg cccagctggc
     1501 cgacgaacgc agccgggcct tcctggccga ggtgctgacc gcctgaggca caccgcctga
     1561 agcacaaggg ggggagggag ccggcccagc gcctgccccc tcccccaaac gcaaagaggc
     1621 ggcggccttc ccggccaccg cccctctcgc gcaaagcctg ttcgcgcgtg aaccgcttac
     1681 tggttcatcg ttgcgaagaa gtcttcgttg gtcttggaat ccttcatctt gtcgagcagg
     1741 aattccatgg catcgaccgt gcccatctgc atgaggatgc ggcgcagcac ccacatcttc
     1801 gagagctgat ccttgggcac cagcagctct tccttgcggg tgccgctctt gcccacgtcc
     1861 agcgccggga agatgcgctt gtcggccacc ttgcggtcca gcacgatttc gctgttgccg
     1921 gtgcccttga actcttcgaa gatcacttca tccatgcggc tgccggtatc gatcagcgcg
     1981 gtggcaatga tggaaagcga gccgccctcc tcgatgttgc gtgccgcacc aaagaagcgc
     2041 ttggggcgct gcagggcgtt ggcatccaca ccgccggtca acaccttgcc gctggaaggc
     2101 accaccgtgt tgtaggcgcg gcccaggcgg gtaatcgagt ccagcaggat gacgacatcg
     2161 cgcttgtgct caaccaggcg cttggccttt tcgatcacca tctcggccac ctgcacatgg
     2221 cgcgcggccg gctcgtcgaa ggtggaggag atcacctcgc ccttcacgct gcgctgcatg
     2281 tcggtcactt cttccgggcg ctcgtccacc agcagcacca gcaggaacac ttccgggtga
     2341 ttgtcggtga tcgccttggc gatgttctgc agcagcacgg tcttgccggt gcgcggcggc
     2401 gccacgatca gcgcgcgctg gcccttgccc tgcgggctga tcaggtcaat cacccgggca
     2461 gacttgtcct tcaccgtggg atcaagcgtg tccagcttca ggcgctggtt gggatagagc
     2521 ggcgtgagat tgtcgaaatt cacccggtgg cgcactgcct cgggatcgtc gaaattcacc
     2581 ttgatcagct tggtgatggc aaaatagcgc tcgccatcct tgggcgcgcg gatctcgcct
     2641 tccaccgtgt cacccgtgcg caggccccat ttgcgcacct ggttgggcga cacgtaaata
     2701 tcatcgggcc cggcaagata attcgcctcg gggctgcgca ggaagccaaa gccatcgggc
     2761 agcacctcga tcgtgccgat gcccaggatc tcttcgccat cctcggccag ttccttcagc
     2821 acggcaaaca tcagatcttg ccgacgcagc gtgctggcgc cttccacgcc cagttcctca
     2881 gccatttcaa ccagttcggc cgaagtcttt ttcttcaatt ctttcaaatg catgttctgt
     2941 attccataag gagttttcgg ccggctggtc catcgggctt ggagaaagcg agacctttgg
     3001 gcagcgcaac acaggcgcgc tgcgggcatt gccgactggg cgggctttta ggcccgccgt
     3061 gcgtgttagt caagaaaagc ggcttgcgcc ggcgatagct gccagccttc agaaaggctt
     3121 caccaccacc atcaccacga tcaccgccgc ggccagtcct ggcacctcgt tcagcatgcg
     3181 caaatgccga cccgacaggc gccgttcccc ccgcgccagt gccccggcat agcgcgccag
     3241 ccagatgtga tagaagctga gccccaaggc caccgccagc ttcaggtgaa accagccatg
     3301 ggcaaaagcg ccaatcgcat gggccagcgc tagccccagc agccacaccg ccaccagcga
     3361 tggccacagg atgatcttca gcagcttctg ctcgcgctcc acccagcgcg cttcctcggc
     3421 cgagccggct tccgcttcct ggtgatagac aaaataacgc ggcagcatga acaggcccgc
     3481 catccagaag atgacgaaaa tgatatgtcc ggccttcagc cagagatagg tcatcgagat
     3541 aatccagtcc atgcaggcac catcttaccg gctccatccc cgcacgatgg cgagaagctg
     3601 ctcgacatgg gcaatcggcg tcgtctgccc gatgccgtgg cccaggttga acacatgggg
     3661 ccgatcggca aaggcttcaa gcacgcccag cgcccgcgct tccatctcgg caccgcccgc
     3721 cagcagcagc agtggatcaa gattgccctg caccggcatg cccgcaggca gggtgcgcgc
     3781 ggcccacaca gggtcgatgg tctcgtctat gcccagcgca tccacgccgg tttcgcgggc
     3841 ataggccgcc agtttctcgc ccgccccctt gggaaagccg atgatcggca catgcggcgc
     3901 gcgcgccttc accccggcca caatggccgc attgggcgcg atcacccagc gctcgaattc
     3961 ggccggggcc aggctgcctg cccagctgtc gaacaattgc accgcttcgg cgcccgcctc
     4021 gatctgcccc agcagatact ccaccgtcac gccaacgatg gcatcgatga tcgcctgaaa
     4081 cgccgcccgg tcgcgatagg cataggcgcg ggtctcatgc tggtcgcggc tgccctcgcc
     4141 gttcaccatg taggtggcga tggtccaggg gctgccggca aagcccagca tggtctgctc
     4201 agggctcagc tgggccacca cctgccgtac cgtctcatag atcggcagaa ggcgctctgg
     4261 cacgctctgg aggccattga gggcatgatc cacaaggcgg ggggcaaggt gcggcccctc
     4321 gcccaccagg aactgcagat cctgccccat ggcatagggc acgatgagga tatcggagaa
     4381 caggatcgcg ccatcgaagc caaagcggcg cagcggctgc aaggtgatct cggcagcagc
     4441 cgcgcaatcg tagaccagcg ccagaaagcc gcccttctct gcccgcaagg cgcgatattc
     4501 cggcagatag cggccggcct ggcgcatcag ccagatcggc ctctgcttcg cattggcacc
     4561 gcgcaacgta tcgatcagca agccgggcat gggtctatcc gattccaaaa aaaagagaat
     4621 ttaaaaggat tctggagtct gttggccctg tggaaactgg ggatggcgcg cccatgcccg
     4681 atttatcccg atctgaacag gctttccgat ggcatgcgac tccgcctgac tcatgagtca
     4741 aacgcaactt atgagtctta tccccagcct gtgggtgaaa gcgcccagcc tgtccacaag
     4801 cgtgcgggct gaacggccaa aacggggatg gattgtgcac ctgcgattgt cacccggttc
     4861 atcccgtggt ttatgccact gattatccac aagccgggga aacgccgcac acatgacgcg
     4921 cctgcacttg catctcctgt cagactcgac cggcgagacg ctggaaatga tcgccaaggc
     4981 cggccttgcc cagttcgacg atgccgatgt ggtgcgccat ttctggccga tggtgcgctc
     5041 gcagcagcat ctcgacagga tcatgggcga tattgccgcg catccggggc tggtgctgtt
     5101 cacgattgtc agcgccgata tccgcaccct gctggaagag cgctgccggg cgatgggcct
     5161 gcccgccgtg ccggcaatcg atgcggtgat cgatgcactg gaaaatgcgc tggggcagga
     5221 atccaagggc cggccg
//
LOCUS       DASRYE010000234         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_243017,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000234 DASRYE010000000
VERSION     DASRYE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243017"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..311)
                     /locus_tag="VFF98_12090"
     CDS             complement(54..311)
                     /locus_tag="VFF98_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZU64415.1"
                     /translation="MPLIVWLLVGGFVGWLAGAVMKNDAGCIGNIVIGIAGAFIGGLI
                     FGRGTINNAPLTISSLVVSVIGAIVLIGLVNLLRQSGGRRR"
     gene            complement(359..2356)
                     /gene="parE"
                     /locus_tag="VFF98_12095"
     CDS             complement(359..2356)
                     /gene="parE"
                     /locus_tag="VFF98_12095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZU64416.1"
                     /translation="MADDLFDGPGAKAEDSYDGSAIEVLEGLEPVRRRPGMYIGGTDE
                     RALHHLAAEVLDNAMDEAVAGHANRIEVILEEAPGTPGRLTIIDNGRGIPVDEHPKFP
                     GKSALEVILSTLHSGGKFSGKAYATSGGLHGVGISVVNALSTLTRVEVARNRELFAQE
                     FSRGQTLGPLQKVGAAPNRRGTSVSFTPDPEIFGADARFKPARLFRLVRSKAYLFAGV
                     EIRWKCAASLVSDDVPAEASFQFPGGLADHLAEQLDSRECATSQFFSGTQDFPGENMG
                     RVEWAVAWPLWSDGAYSWYCNTIPTPDGGTHEQGLRAALTKAVRAFGELVGNKRAKDI
                     APEDIVTGCEAMLSVFIRDPQFQSQTKDRLTSPEAARLVENAVRDHFDHFLTDNMERG
                     KALLGYVMERMDERLRRKAEREVKRKTATNARKLRLPGKLTDCSGEGEGETELFIVEG
                     DSAGGSAKQARNRKTQAILPIRGKILNVASASADKIRANQEIADLTLALGCGIRKECN
                     PDHLRYDRIVIMTDADVDGAHIATLLMTFFFQEMPEVVKRGHLYLAQPPLYRLTAGKE
                     SAYARDDAHRAELEATRFKGKRVEVGRFKGLGEMNPQQLRETTMDPAKRGLLRITLPP
                     EYEGRAAVKDLVDRLMGRNPEHRFHFIQNRAAEIDPEMIDA"
     gene            complement(2464..>3575)
                     /locus_tag="VFF98_12100"
     CDS             complement(2464..>3575)
                     /locus_tag="VFF98_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZU64417.1"
                     /translation="MAGLLAACQVIPQGPHEAPPPPAQGSAIALPRDGARHRVALLVP
                     LGAGGQAGAGQAIANAATMALLDTNADTIRITTYDTSAGVSAAAARAIADGNKLILGP
                     LFTEETQAVAMAARPARVPVIAFANDAGLAGENLFIMGTPPEQPIARVVRQARAAGVQ
                     RFAALIPHGAYGTRASSAMIEAVRANGGTLVGMEGYERTPASVQAAARRLKAHGPIDA
                     VLVADEPRVAIDAASVLKAGNPQLRLLGTDLWTGDAAIARSPLFNGALYAALSDQRYA
                     RFAESYKSRYGTAPHRIATLGYDSVLLTLRLARDWPAGGPFPTARLFDHDGFLGLDGV
                     FRFDVADVIQRALEVREVRRTGVAVVSPAPARFAN"
BASE COUNT          551 a         1195 c         1242 g          587 t
ORIGIN      
        1 ggccggccgc tggcctgaag gcagcgccgg cgtgacaggc tgcgccggcg ggctcagcgc
       61 cgccggccac cgctctggcg caacaggttg accaggccga tcagcacgat cgccccgatc
      121 accgagacga ccaggctgga gatcgtcagc ggggcgttgt tgatcgtgcc gcggccaaag
      181 atcagcccgc cgatgaaagc gccggcaatg ccgatgacga tattgccgat gcagccggca
      241 tcattcttca tcacagcgcc ggccagccag ccgacaaagc cgcccaccag cagccagacg
      301 ataaggggga tcatggagga aggacctttt ctcttgtgcg ctcaggttct ctgggggctc
      361 aggcgtcgat catctcgggg tcgatctcgg cggcgcggtt ctggatgaaa tggaagcggt
      421 gctccgggtt gcggcccatc agccggtcca ccagatcctt caccgccgcg cggccctcat
      481 attcgggagg cagggtgatg cgcagcaggc cgcgcttggc cggatccatg gtggtttcgc
      541 gcaattgctg ggggttcatt tcgcccaggc ccttgaagcg gcccacttcc acgcgcttgc
      601 ccttgaaccg ggtcgcttcc agctcggccc gatgggcatc gtcgcgggca taggcgctct
      661 ccttgccggc ggtgaggcgg tagagcggcg gctgggccag atagagatgg ccgcgcttca
      721 ccacctcggg catttcctgg aagaagaagg tcatcagcag ggtggcgata tgggcaccat
      781 cgacatcggc gtcggtcatg atgacgatgc ggtcgtagcg cagatgatcg gggttgcact
      841 ccttgcggat gccgcagccc agcgccagcg tcagatcggc gatttcctga ttggcgcgga
      901 tcttgtcggc gctggcgctg gccacattca ggatcttgcc gcggatgggc aggatcgcct
      961 gggtcttgcg gttgcgggcc tgcttggcgc tgccaccggc cgaatcgccc tccacgatga
     1021 acagttccgt ctcgccttcg ccctcgccgc tgcaatcggt cagcttgccg ggcaggcgca
     1081 gcttcctcgc attggtggcg gtcttgcgct tcacctcgcg ctccgccttg cggcgcaggc
     1141 gctcgtccat cctctccatc acatagccga gcagcgcctt gccgcgctcc atattgtcgg
     1201 tgaggaagtg gtcgaaatgg tcgcgcacgg cgttttccac cagccttgcc gcttccgggc
     1261 tggtcaggcg gtccttggtc tggctctgga actgggggtc gcggatgaac accgagagca
     1321 tcgcctcgca gccggtgacg atgtcttcgg gcgcgatatc cttggcgcgc ttgttgccca
     1381 ccagctcgcc aaaggcgcgc accgccttgg tgagggcggc gcgcaggccc tgttcgtggg
     1441 tgccgccatc gggcgtgggg atggtgttgc aataccagct gtaggcgcca tccgaccaca
     1501 gcggccaggc caccgcccat tccacccggc ccatgttctc gccgggaaaa tcctgcgtgc
     1561 ccgagaagaa ctggctggtg gcgcattcgc ggctgtccag ctgctcggcc agatgatcgg
     1621 caaggccccc ggggaactgg aagctggcct cggccggcac gtcgtccgat acgagggaag
     1681 ccgcgcattt ccagcggatt tccacgccgg cgaagagata ggccttggag cgcaccagcc
     1741 tgaacagccg cgccggcttg aagcgggcat cggcaccgaa aatctcggga tcgggcgtga
     1801 agctgaccga agtgccgcgc cggttgggcg ccgcgcccac cttctgcagc ggccccagcg
     1861 tttgcccgcg cgagaattcc tgggcgaaca attcgcggtt gcgggccact tccacccgcg
     1921 tcaacgtgga cagcgcattg accacgctga tgcccacgcc atgcaggccg cccgaggtgg
     1981 cataggcctt gccggaaaac ttgccgcccg aatggagcgt ggagaggatc acttccagcg
     2041 cgctcttgcc ggggaacttg gggtgttcgt ccaccggaat gccgcggcca ttgtcgatga
     2101 tggtcagccg gccgggggtg ccgggggctt cctccaaaat cacctcgatg cggttggcat
     2161 ggccggccac ggcctcgtcc atcgcattgt cgagcacttc ggcggccaga tggtgcaggg
     2221 cgcgttcgtc ggtgccgccg atatacatgc ccgggcgccg gcgcactggc tccagcccct
     2281 ccagcacctc gatcgccgag ccgtcgtagc tgtcctcggc cttcgcgccg ggcccgtcga
     2341 agagatcatc cgccatggcg cgcgttctag cttggccgcc cctgccaagg caagcggcgc
     2401 ggcgcgctta tcgccagatg ttttgccttt gttcgacagg agcaggcccg cttgcccgca
     2461 atttcaattg gcaaagcggg ccggcgccgg gctgacaacg gccacgcccg tgcggcgcac
     2521 ctcgcgcact tccagcgctc gctggatcac atcggcgaca tcgaagcgga acacgccatc
     2581 gaggccgagg aagccatcat gatcgaacag ccgggcggtg gggaaggggc cgccggccgg
     2641 ccagtcgcgc gccagccgca aggtcagcag cacgctgtcg tagcccagcg tggcgatgcg
     2701 gtggggggcg gtgccatagc ggctcttgta gctttcggcg aagcgggcat agcgctggtc
     2761 cgacagggcg gcatagagcg cgccattgaa cagcgggctg cgggcaatcg cggcatcgcc
     2821 ggtccacaga tcggtgccca agaggcgcag ctgcgggttg ccggccttca gcacgctggc
     2881 cgcatcgatc gccacgcgcg gctcgtcggc caccagcacg gcatcgatgg ggccatgcgc
     2941 cttcagccgc cgggcggccg cctgcaccga ggcgggcgtg cgctcatagc cttccatgcc
     3001 caccagcgtg ccgccattgg cgcgcaccgc ctcgatcatc gccgaggaag cgcgggtgcc
     3061 ataggcgcca tgggggatca gcgcggcaaa gcgctgcacg ccggccgcgc gggcctggcg
     3121 caccacgcgg gcaatcggct gttcgggcgg ggtgcccatg atgaagaggt tctcgcccgc
     3181 cagccccgca tcattggcaa aggcgatcac cggcacacgg gcgggccgcg cggccatcgc
     3241 cacggcctgg gtctcctcgg tgaacagcgg gcccaggatc agcttgttgc catcggcgat
     3301 ggcgcgggcg gcagcggccg aaacgccggc cgaagtgtcg taggtggtga tgcggatggt
     3361 gtcggcattg gtgtcgagca gggccatggt ggcggcattg gcaatggcct ggccggcacc
     3421 ggcctgcccg cccgcgccca gcggcaccag cagtgccacc cggtggcgcg cgccatcgcg
     3481 cggcagggca atggcgctgc cctgggcggg cggcggcggc gcttcatgcg ggccttgcgg
     3541 aatgacctgg caggccgcca gcaggccggc catca
//
LOCUS       DASRYE010000235        29668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_155019,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000235 DASRYE010000000
VERSION     DASRYE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 29668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29668
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155019"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2209)
                     /locus_tag="VFF98_12105"
     CDS             complement(71..2209)
                     /locus_tag="VFF98_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64418.1"
                     /translation="MISNRYPYLLSPGRIGAMETKNRILVTAMGVSLAEDDGSVGAQL
                     LAYHEEQARGGAGLIITGVTGVAWPVGSVTRQQTAISDDRFIPGLARLCAAVHAHGAR
                     IAAQLHHGGLAAAYAAGRWGHPLWGPAVPPAPKGSFTDYFLMEELAGLAGMKAPAIKV
                     MDEADIAVVIDQFVQAARRAQIAGFDGIEFHAGHGYLLSSFLSPITNTRTDRYGGSVE
                     NRARLLLEVLAAVRAEVGADMPLWVKLDSREVGKPGGITIEDAIVTARLIEAAGANAI
                     TVTTYHETSQGKLHSQSHTPHIENYNLEASTRIRQQVAIPVISSGRIEPEAGDSAIGK
                     GQVDFIAMGRKLLADPHLPAKLAAGQRERLRPCIYCYTCISAIYMGQQVRCAVNPELA
                     HESEAQGLPAGPVKHVVVVGGGPGGMEATRRLAARGHRVTLLEKAPRLGGTVRFAGLA
                     YEPNERLLKWLIGEVEQSGAEIRLDTLATPRLVADLAPDAVVVATGAQRGMPPLPGND
                     LPHVFSGDDMRAMMFGETSPELKRKTGLFTRLATRVGAATGASANLDLVRKATHSWMP
                     LGQRIVIIGGELVGLELAEFLHERGRQVAVVEAAPRLGKGLMLVRRMRMLQELAEHGV
                     ALHAGAADIAITPEAVTFSTGGTPQALAADTVIVAQGATADLALADALRAAGLAVHAI
                     GDAGGVGYIEGAMRDAYDAVRTIEGWGKPE"
     gene            complement(2398..4953)
                     /locus_tag="VFF98_12110"
     CDS             complement(2398..4953)
                     /locus_tag="VFF98_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64419.1"
                     /translation="MHVHRYRLLLGAGAMALGLANPASAKADTTAQDHVKPADNQLGE
                     IVVTAQKRKENLQVVPVAVSAISAEKIAKIGVSDARDLSGIAPNVTIAPGTTSNSAGV
                     MSIRGISSGSSETFGLDAANGLYVDGIYIARAAEAGLDVADIERVEVLRGPQGTLFGR
                     NTTGGAISFVSRKPSDTFRLTAEVGYGNYNAWNGKISVDPGEIAGIDTSFLFVHRERE
                     GVVTNLDQPDRSRDPGAFRSNGFRAAARAHLGTSGTIQYIFDMSQTIGSPMNFQLTNT
                     SNGAYNAPISVNGQQVTVQQQAPVSQYLAGVTFANPACAALATPQRAWRNTVCNDITN
                     YSNDKMWGHNLQVENDFGSFKLKSTTGYRIWHNTNVADLDGIGAFSGAQYTSASLFNG
                     MPAALLQYIPSIPTAYDAAIAASAVPTITQNLFTSADQRSQRQLSQELELSGSSDRFD
                     WVVGGFYFWEKGIEYNPQNSGYVLDTNASVFTAANFGALASSFAAANPAQYRLVQTLT
                     VLNYQTTSQSEAAYAQATFYPGGHHGRWRLTLGGRYTWDQKSMLRFQNGSTPDTTPET
                     GNANFSRFTWNAMLGYDFAKGITGYARVATGYRSGGFNAADPSITGTNTLAHFNPESV
                     TSYEVGLKTELLDRHLRLNVAAYHNVYENLEVTVPVVSASGGTFVSRVQNAGRVHYDG
                     IEADAQAILSRYVSIDGSIGYVSTKFIEFNSGLSTTGATVNVASLAVGTYSSPLTANI
                     ALDAHYPLGRGDARLFGRIGYTHEDGQYSFSQVLTSPFNNAIKGDARNLVDLQFGVDR
                     FKVGTAEAQLKFWVKNLTDEHALTRGIDFGALGYAGGYYELPRTYGVSLGVKL"
     gene            complement(5113..5874)
                     /locus_tag="VFF98_12115"
     CDS             complement(5113..5874)
                     /locus_tag="VFF98_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU64420.1"
                     /translation="MLLPALGADLPGGAVLAGCLLATGLAGALRGFTGFGFGLAATPL
                     LSLVLAPGELVPVVLLLQVASSLVGLRQTLAAHEPGSTRWLSLAAMLTTPLGVWILHH
                     IGLAAARLCIAGVSAGATLVLAGGWRHTRPHGPGFILPFGLLAGVLSGLCAMPGPPVL
                     AYYLAVGTPPAAARASMILIFLVTGGLALAGNLWVAGLGAPVLLLAGLCLPAMMAGTL
                     AGESLFRLSGGRHYRQIGLACLVAIAASAALRALL"
     gene            complement(5878..7374)
                     /locus_tag="VFF98_12120"
     CDS             complement(5878..7374)
                     /locus_tag="VFF98_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU64421.1"
                     /translation="MAHILSDTPDMAAHGTAPIRAGHLINGQLVAPAGDLFAVINPAT
                     GAVLGHAAAGTAADVDQAVQAAAAAQPGWAALPARERGRLVGEAARRLLAQTEPLARL
                     LALETGKAIRTECRPEILTAADILAFYAGLGSELKGETLPFRPDLLCLTTREPIGVVA
                     AILPWNVPLVLMMLKAAPALVAGNAVVVKSAEEAPFATLEAARLMAEVLPAGVLNVVS
                     GFGPACGQPLVEHPLVGKVTFTGSQAVGETIYATAARKIVPVSLELGGKSPMIVMADA
                     DLAKAVAGAIAGMRFTRQGQSCTAASRIYVHEALHAPFVAALEEALQALRIGDPLDEA
                     TDIGTIISPEQMATIARYIEIGRQTPGARVIECGQPPAGAGLDPALFALPTLFTGLPE
                     DSPLLREEIFGPVACIQPWRDYEEVIAAANDSDFGLAASIWTNDLSAALDATRRLDAG
                     YVQVNQNLTIQPNLSYGGFGKSGLGKEASLEAMLEHFTRKKTIVFAAA"
     gene            complement(7460..8941)
                     /locus_tag="VFF98_12125"
     CDS             complement(7460..8941)
                     /locus_tag="VFF98_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZU64422.1"
                     /translation="MRKRGAILWQRLLAIDASDARPLQVQLRTRVVDAILSGTLAPGT
                     AMPSSRGLASELAVSRNTVILSYQQLVDDGFLESRERSGFVVARNAAAGWVSQAPARA
                     PAQRIDLGARVRSRAAALRHIEKPSNWHTYRFPFLYGQVDAGAFPIAEWRQCCRQALS
                     VLELRDWAPDKIDEDDPLLIEQLRSRILPRRGIWAERDEIMVTLGAQQALYLLAEALF
                     GPETRVGLEEPGYPDMRNIAAMRTRHLCRLPVDAAGLQPDADLLACDYVHVTPSHQCP
                     TTVTMPYERRMALIEAVQRSSTVLIEDDYDSETSFEAAHRPALKSLDRTGQVIYVGSL
                     SKLLAPGLRLGFVVADAALIRELRALRRLMVRHPPLNNQRSVALFLSYGHYESLVRRL
                     TATYRARAGQLVAALSALPGVDFVPPTGGSCLWLRGPKGFDAQAAAQAARAEGLLFEP
                     GDIFFATPDSPGRHIRLGYSVIPERRIAAGIALLGEIIARQMG"
     gene            9088..10419
                     /locus_tag="VFF98_12130"
     CDS             9088..10419
                     /locus_tag="VFF98_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU64423.1"
                     /translation="METQAPPPALRPDLAHHWMPFTANRDFREAPKMILRGQGVVYYG
                     EGGQPILDGCSGLFTCPAGHSRPEIAEAVRDQLLTLDYAPSFTRGHPLSFRAAERIAA
                     LTPAGLDRIFFANSGSEAVETALKICLAYHRARGEGTRSLFVSRERAYHGVNFGGVAL
                     SGMVPNRRAFGGAGPQVVHMRHTWLEENRFTPGQPVHGAELAEDLARFVALHGAENIA
                     ACVVEPIAGSTGVLVPPPGYLERLREICTQNGILLVFDEVITGFGRTGHAFAAQAFGV
                     VPDLITMAKAITNGAQPMAAVAVSRHVHDTIMEAAPEGQVEFFHGYTFSAHPAACAAS
                     LAALEIYETEHLFDRARQLSAYFLDAVMTLADLPVVTDIRGFGMLAGIDMAPAERPGL
                     RGHAVQKRLFDAGLHLKTTGDAALLAPAFVTETGQIDAMVGIVREVLSALG"
     gene            10652..12829
                     /locus_tag="VFF98_12135"
     CDS             10652..12829
                     /locus_tag="VFF98_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64424.1"
                     /translation="MLCCAASWALALPGIAQAQQAQQAQGGLGDIVVTAQKRTESAQG
                     VPLSIQSLGAQKLEALGAAKLQNIEMAAPSVSFGDGSEQGRAGIRGVIDYSRNAGYDS
                     RVGIYVDGVYYSRSWMNNLTLLGVQQVDVLRGPQGTLFGKNTDAGAIAITTLQPGRRT
                     EGEVQAEVGNFGYWKLGGRLAAPVGSDLAMQLAATHVESRGYYHNSYTSQRNQGLHGD
                     AVAGKLRYQPGAGIDITLAGDYTRDRNTTQHYTAPPAAGGDPYTFRSYYNDHANRDMG
                     GLSLSARVPLGGGYDLTAITAWRGGQQDLAFNNETGTTPYLTALLNQHTRQFSQEARI
                     ASPRRRTYDFVAGVYYFWANNQDDYTSLWGSGLAQLGSVYGLYAGASTGAHASVSTQS
                     VAGFVQGSWRPGRVIELFAGGRLTYEQKRLNDLTTHDPYGYFLAGLHGYADSLSNSFF
                     TPRGGVNLHLTPHVLVFGTVSRGFKSGGWNVEGTSLAAQAAGIRFRPEKVTSYEIGLK
                     SDFLDRRARFNLTGFYERFTDFQVFTFVPTTLYGVTSLTSSLTNAGAVSSQGGEVELA
                     VIPVRGLTVSANYTYDVSRFDRYPGGGGTVAGVPLDAHGVQTPYAPRHKLYAALDYAG
                     QLSPAVGITAHFGYSAQSSENFDPKVINPAYGRYYFIQGYDLADARLGLAGTHSRWSA
                     ALWSKNLFDRRYVLFANRTALLQAPAVMYGEPRSFGLTVGYRF"
     gene            complement(12873..13802)
                     /locus_tag="VFF98_12140"
     CDS             complement(12873..13802)
                     /locus_tag="VFF98_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HZU64425.1"
                     /translation="MREPPVPVPVPVPATILAGYLGAGKTTTVNHLLRHAGGRRLLVL
                     VNDFGALPIDGDLIAGREGGVITLANGCACCQVGQDLLGALARALAGPARPDHLLIEA
                     SGIADPVQLAQLAQAEPLLRLDAVATLVDAAGIRQRLADRWAGATVRRQIGAAGLLVL
                     NKADLVAPEELAALCALLADLAPGAARLATRQGRVAPDLLLGPAGWRSAPGPTGAGHG
                     YRSWSGRAGGAIPPAAMRAFLAALPEWVHRVKGIAPDPAGSAYACHRVGRQVAIEAAP
                     AGTDPAARIVAIGPAGPFAPAVLESAWAALCGS"
     gene            complement(13802..15424)
                     /locus_tag="VFF98_12145"
     CDS             complement(13802..15424)
                     /locus_tag="VFF98_12145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU64426.1"
                     /translation="MPPITLFSARAILTMDRNCPRATHVAVREGKVLGVGTAESLAGW
                     GPALLDTRFADKVLLPGFVEGHAHAMTGHLWKDTYLGHMERIDPAGRRWAGLGETEAV
                     VARLSAATAARAGVLMGWGFDPLFVAGERLSRQHLDRVSATRPVIVLHSNGHLMTVNS
                     AALALAAYDRTTCVPGVCKDAAGEPTGELHEMAAMGPVFMRLGLAGLQSGEPTYRAFA
                     ELARRAGVTTATDLGATIAAADVPALRAFIDRPDFPLRLAVMLMAHHRPPAEILAEAL
                     ALAPLGSDKLRLGAVKIVLDGSIQGFTAQLRWPGYYRGTDHGLWNIAPEQLFELVAML
                     NAAGVQIHIHTNGDLATDLALEAFAAALARTPRFDHRHTLQHCQMADAAQFRRMKALG
                     LACNLFANHLYYFGDKHYELTIGPDRAERMNACATALELGVPFAIHSDEPVTPIGPLF
                     TAWCAVNRRTQEGRVLGAAQRISVEAALRAITLGAAHTLHMDDTIGSIEIGKWADFTV
                     LDEDPTAVHPMALREIGIAGTVLAGQPTGALA"
     gene            15582..16859
                     /locus_tag="VFF98_12150"
     CDS             15582..16859
                     /locus_tag="VFF98_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64427.1"
                     /translation="MRFKIFPGWLLVGAVLVALMLSGATVIVSSFGIVTAHLTDRFGW
                     SQVTMAGALSVALLATTLAVPLVGLTVDRFGTRRTAIAGSLAFAAVLASAGLMVRSLP
                     GFYGFYALLGVVGAFTNPIVYIAALSAWFDRQRGVALGLAVAGQGLGGAVLPVGIETI
                     ARQGGWAMAFAGMAVAVVLVAVPLILAFVHDHPAARGCLPDHGHSRPGGQAGPGGPAG
                     AAAPHGLSLAEALRCRSFWIIMAVLALLGLTTYAYSGHLVALMRARHIATIGQAALLA
                     SVSGATMILSRVAFGWLFDRFALPLVGAGGIALCALAQFLLLRLDHFGPMVLVMGMLF
                     GASMGAETDLLTLLVGRYFGTRALSRIYSWQNVAFLAGAAVGPVLFSALMQGLGGVEL
                     PVFVMMGLTVLAAALLLGLHREPQHGRAAQALA"
     gene            complement(16881..17225)
                     /locus_tag="VFF98_12155"
     CDS             complement(16881..17225)
                     /locus_tag="VFF98_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU64428.1"
                     /translation="MTIQRIDPGPRMSEAVIHNDTIYLAGQIGDPASDAAGQTREALA
                     EIDALLARCGSSKAKILSATVWLADIADFDAMNTAWDAWVDPAHPPARATGEARLFAP
                     GYKVEIIVVAAR"
     gene            complement(17267..18526)
                     /locus_tag="VFF98_12160"
     CDS             complement(17267..18526)
                     /locus_tag="VFF98_12160"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HZU64429.1"
                     /translation="MKIAILGAGVIGVTSAHYLARAGHEVVLVDRQPAPALETSFANA
                     GEISPGYASPWAAPGIPLKALKWLAMRHAPLILRPGLSPAAWGWVARMLGQCNAPAYA
                     ANKARMVRLAEYSRDCLDALRQETGIAYDGRQQGTLQVLRTQGQLDAIGKDMAVLSSY
                     GVPFELLDRAGCIAAEPGLAGARARIAGGLRLPHDETGDCFLFTRALAEQARALGVEM
                     QMGVAVHALEAAGGRITAAHTSAGRIVADAFVVALGSFSPALLRPLGIGLPVYPVKGY
                     SLTLPVADAAAAPQSTVMDETFKVAITRLGSRIRVGGIAEISGFNRQLRTAALATLRK
                     SLADMFPTAADAGADPQFWTGLRPMTPDGTPVIGATPLGNLFLNTGHGTLGWTMACGS
                     ARLLADVICGRPPEIAHADLAIARYRA"
     gene            complement(18523..19656)
                     /gene="alr"
                     /locus_tag="VFF98_12165"
     CDS             complement(18523..19656)
                     /gene="alr"
                     /locus_tag="VFF98_12165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZU64430.1"
                     /translation="MALPPPPAGALLTIDLAALVANWRHIGAASGAAQVAAVVKADAY
                     GLGARPVVRALAAAGCRLFCVAHLEEALALADLLPQGGALGVLNGLVPGAEGACARAG
                     LRPVLNTLAQARRWQAEAATQHRPLPAFLQVDSGMARLGVSPAEAALLATDTAFRAAV
                     PLAGLISHLACADVPEDPANARQLASFAATAAHFPGVARSLANSGGCLMGPAFGGDIA
                     RPGLALYGAQPAIHPDPALRPVVRLDARVIQIRDLPAGAGAGYGLSWQAPAPARLAAL
                     AVGYADGWPRSLSNRGFAAFGGQILPIAGRVSMDSTLVDITALAPDALAEGDLVELIG
                     PNRPIGQVAAEAGTIAYEILTGLGPRYARRYLGAAPLATEQMP"
     gene            19800..20258
                     /locus_tag="VFF98_12170"
     CDS             19800..20258
                     /locus_tag="VFF98_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU64431.1"
                     /translation="MPTLDKADREILRILQVDGRISNQDLAQKVGLSPSACLRRVRLL
                     EGRGIIRGYAAQVAASEETEATVVIVRITLEKQTEEYLRRFEHALRQYPEIKECYLMT
                     GDADYTMRVATGSAADYEHIHTHILSRLPGVARIHSSVAMRNVLGAGRRR"
     gene            complement(20255..20851)
                     /gene="cobO"
                     /locus_tag="VFF98_12175"
     CDS             complement(20255..20851)
                     /gene="cobO"
                     /locus_tag="VFF98_12175"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZU64432.1"
                     /translation="MQRHAAKMAKKKAGRDRIMAGKTGEKGLVIVHTGTGKGKSSSGF
                     GMILRCVAHGMPCAVVQFIKGAWDTGERRLIESHFADLCQFYAMGEGFTWETQDRARD
                     IAAARQGWEKAKALIRDPAIRMVLLDEINIALRYDYLPLDEVLAFLREEKPELTHVVL
                     TGRNAAPELIEQADLVTEMTLVKHPFRSGIKAQAGVEF"
     regulatory      20949..21185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            21278..21643
                     /locus_tag="VFF98_12180"
     CDS             21278..21643
                     /locus_tag="VFF98_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HZU64433.1"
                     /translation="MENEATLFVCATCRAGDPQATGPKSAGARLAAALIAAHDGAPGL
                     AIRPVECLSACNNGCAIALSRPGAWTYVYGRLGEGDVPQILSGAVAYAASADGIVPWR
                     ERPDIFRKQSLARIPPLEA"
     gene            21647..22705
                     /gene="cobW"
                     /locus_tag="VFF98_12185"
     CDS             21647..22705
                     /gene="cobW"
                     /locus_tag="VFF98_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HZU64434.1"
                     /translation="MSLEKSLEKIPVTIVTGFLGAGKTTLIRHLLQNPGGRRLAVLVN
                     EFGTVGVDGEILKACADENCPESAILELANGCICCTVADDFIPTVEQLLALPQRPDHI
                     LIETSGLALPKPLLKAFDWPAIRSRITVDGVIALADAEAVAAGRFAPDVAAVDAQRAA
                     DDSLDHETPLSEVFEDQLACADIVLLAKADLVDEAGLAAARAVIAAEAPRPLPVLPVT
                     DGAIDPAIILGLNAAAEDDLAARPSHHDGEDEHEHEDFDSTIVSLGEVADPEALVAAV
                     QQLARAQGVLRVKGFVAVHGKPMRLLLQAVGERVRHQYDQPWGARPRTTQLVVIAEHD
                     RLDRAAVQAVLGGALAPA"
     gene            22710..25964
                     /gene="cobN"
                     /locus_tag="VFF98_12190"
     CDS             22710..25964
                     /gene="cobN"
                     /locus_tag="VFF98_12190"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HZU64435.1"
                     /translation="MHVVFRERRGLEDTGIATDLGQSPAPLVVLSFSDSDLGAFAAGW
                     HAAGGRLPALRLANLAALTHPLSVDTYIARTLQGARAVLVRLIGGAGYWPYGLGQLQD
                     LARRQGLALAVLPADGREDGVLDGFSTLPVPVLRRLAGLCDAGGAAAARAALGELARA
                     AGLPAPKAPEPQPQALMLPACGWYLPGAGVVAGPPPGAGPLAVVSFYRAYLASADMGA
                     VDALLAALAERGFRAAGLFAPSLKAPGAVAWITGQLACHRPAAIVNATAFSARDEAGA
                     SPLDGPGCPVFQVALSTAGREVWAAAERGLSPADLAMHVALPEVDGRLFAGVASFKQA
                     GRADPALEFARVCHEADPALVAAIADRVAGWHRLASTTPAARQLAFVLSTYPGRADQM
                     AHAVGLDALASTEAMLAALAAEGYDVAPGPPLAGALQAEEVSWPLAAYRAALAGLPQA
                     LRDDLARAWGEPESDPAVADGAFRFAVARRGAMLVALQPERGPAAAREGDYHDLSRTP
                     CHGYVAFYLWLRAQGVQALVHVGAHGTLEWLPGKAVALSAACWPQALIGALPVIYPFI
                     VNDPGEAAQAKRRLGAVTLGHVPPPQGRAEVPPALLRLERLLDEYSTAEGLDPARRDR
                     LIGAIRAEAQASGVEADLGLAAGASAAEAMVRIDRFVCDIKESQFGDGLHIYGAAAGE
                     MAGLLRALDGRRVAAGPAGSPARGRQDVLPTGRNLYTIDPRAVPSRVAHTQGVKLAEE
                     LLRRHLQDHGDWPRGLVVDLWGSASMRTAGEEFAMALHLAGLAPRWDAGSERVSGFEI
                     LPPALLGRPRIDVTLRVSGLFRDVFPGLAQLFEAASAALAERCEEGPGENPYLARAPR
                     VFGPQPGQYGLGMADPASHLGDAARLAAGEAWLSASAWRIDAQGAIHHDRQALATRLA
                     GADAFAHVQDLPETDLLMARDYAAHEAGFAAAKAALGQAAPALYHLDATRPGAPVARS
                     QAEEIARVVRARAANPAWASGMMRHGWRGAAEIAATLDNLAGFAQLAGQVGPHLFDLY
                     YEATLARADLVDFMARENPQALAELRARFAALVAAGLWTTRRNSIAAALAGGAA"
     gene            25961..27136
                     /gene="cobG"
                     /locus_tag="VFF98_12195"
     CDS             25961..27136
                     /gene="cobG"
                     /locus_tag="VFF98_12195"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HZU64436.1"
                     /translation="MSAPAIRGWCPGALRPMASGDGLVVRLRLPAGRMTGPQARAIAD
                     LARRFGNGLIDVSARANLQLRGVQPDGHPPLLAALAELGLVDADAGAEMRRNVLLAPF
                     WQAGDGAGELALALGAALALGAALAGAQDLALPGKFGFAVDGGALPVLRGASADIRLE
                     RAAGGRWLVYADGAGLGQVVAGAEAVAAALALARWFLATGGVEQGRGRMAAHVRRCGA
                     LPDEANSAVPQAPAFAPVPGPVSAGVLVGVAFGRLSAEVLAALAEAGPIRLTPWRMLL
                     LEGCRQAPALAGLVTLADDPRLRLAVCPGAPQCTQALAEVRDLALALAPHVPAGQVLH
                     VSACAKGCAHPAPADLVLAARPGGFGLARGATAATAASCPPIARAALLSHPELLQAE"
     gene            27139..27768
                     /locus_tag="VFF98_12200"
     CDS             27139..27768
                     /locus_tag="VFF98_12200"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZU64437.1"
                     /translation="MPHVYEQDGAAIYRQSFATIRAEADLARFSPTEEQVVVRMIHAT
                     GLVGLEAQAAFTPGMAEAARGALAAGAVILCDARMVSEGVTRARLPADNRVICTLHDP
                     AVPEMARAMGNTRSAAALELWRPHLAGSIVAIGNAPTALFHLLNMLEDPACPRPAAII
                     GCPVGFVGAMESKQALMAAPPCPAMAVHGRLGGSAMTVAAINALATEKE"
     gene            27765..28526
                     /locus_tag="VFF98_12205"
     CDS             27765..28526
                     /locus_tag="VFF98_12205"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZU64438.1"
                     /translation="MMGKIICVGLGPGDPDLMSVRADRLVRAAAHVAYFRKAGRAGQA
                     RRIVAGMLAADVAEYPMEYPVTTEIAFDSAAYNELLAAFYDDWAQRLAALASDADVVV
                     LCEGDPFFYGSFMHLHARLQGQVAIEVVPGITGMTGCWHATGLPITWGDDVLSVLMGT
                     LPEAELVRHMRQADALAIMKTGRNLPRVRRALAAAGRLEDAWLVERGTMPGQRVLRLA
                     EADPADCPYFAIVLVHGQGRRPQAVAGAAVQELPA"
     gene            28523..29257
                     /gene="cobJ"
                     /locus_tag="VFF98_12210"
     CDS             28523..29257
                     /gene="cobJ"
                     /locus_tag="VFF98_12210"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HZU64439.1"
                     /translation="MSGSVVVAGLGPGDGALITPEVTTALARATDILGYIPYVARIAP
                     RPGLTLHPSDNREELERAAHALDLAAAGRHAVIVSSGDPGVFAMASALFEALEERPAC
                     HDLPIAILPGITAMLAAAARAGAPLGHDFCAINLSDNLKPFALVEHRLRMAARGDFAM
                     AFYNPRSRSRPHQFARVLEILREELAGERLILFARAVCTAEEEIRTTTLAEARPEMAD
                     MRTLVLVGNAATRQVGRWVYTPRSAE"
     gene            complement(29233..>29668)
                     /locus_tag="VFF98_12215"
     CDS             complement(29233..>29668)
                     /locus_tag="VFF98_12215"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HZU64440.1"
                     /translation="PGDRWQPVADMAAACAALPQAPARVFLAIGKQQLDVFAAAPQHV
                     YLLRLVDPPAAPLPLPHAEVVIDRGPFGVEGDIALLRRHAITHVVAKNAGGAGASAKL
                     AAARALGLPVLLVERPQLPPRRVLGEVNAVLGWLAHSADLGV"
BASE COUNT         4328 a         9879 c        10802 g         4659 t
ORIGIN      
        1 tcgtaaaaaa atatgccaac agatacgcaa atggtcaatt gaaaagcttg tcggctttgg
       61 atagcggaat ttattccggc tttccccatc cctcgatggt gcggacggcg tcataggcgt
      121 cgcgcatggc cccttcgata tagccaaccc cgccggcatc gccgatcgcg tgcacggcaa
      181 ggccggcggc gcgcagcgca tcggccagcg ccagatcggc ggtggccccc tgggcaacga
      241 tcaccgtgtc tgcggccaac gcctgcgggg tgcccccggt gctgaaggtc acggcctcgg
      301 gcgtgatggc gatatcggca gcgccggcat gcagggccac gccatgttcg gccagttcct
      361 gcagcatgcg catgcggcgc accagcatca gccccttgcc caggcggggc gcggcttcca
      421 ccacggccac ctggcggccg cgctcgtgca ggaattcggc cagttccagc cccaccagct
      481 cgccgccgat gatgacgatg cgctggccca gcggcatcca gctgtgggtg gccttgcgca
      541 ccagatcgag gttggcgctg gcgccggtgg cagcgcccac gcgggtggcc aggcgcgtga
      601 acaggccggt cttgcgcttc agctccggcg aggtttcgcc gaacatcatc gcccgcatgt
      661 catcgcccga gaagacatgc ggcagatcat tgcccggaag cggcggcatg ccgcgctgcg
      721 cgccggtggc caccaccacc gcatcggggg cgagatctgc caccagccgg ggcgtggcca
      781 gcgtgtcgag gcggatttcg gcgcccgatt gctccacctc gccgatcagc catttcagca
      841 gcctttcgtt gggctcgtag gcaaggccgg caaagcgcac cgtgccgccc agccgcggcg
      901 ccttttccag cagggtcacc cggtgccccc gcgcggcgag gcggcgggtg gcctccatgc
      961 cgccggggcc gccgcccacc accaccacat gcttcacagg gccggcgggc aggccctgcg
     1021 cctcgctttc atgggccagt tccgggttca ccgcgcagcg cacctgctgg cccatgtaga
     1081 tcgcgctgat gcaggtgtag caatagatgc aggggcgcag cctttcgcgc tggccggcgg
     1141 ccagcttggc cggcaggtgc ggatcggcca gcagcttgcg gcccatggcg atgaaatcca
     1201 cctgcccctt gccgatggcg ctgtcgcccg cttccggctc gatgcggccc gaggagatca
     1261 ccgggatcgc cacctgctgg cggatgcggg tgctggcctc gaggttgtag ttctcgatat
     1321 gcggcgtgtg cgactgggaa tgcagcttgc cctggctggt ctcgtgatag gtggtgacgg
     1381 tgatggcatt ggcgccggcc gcctcgatca ggcgggcggt gacgatggca tcctcgatgg
     1441 tgatgccgcc aggcttgccc acctcgcgcg aatcgagctt gacccacagc ggcatgtccg
     1501 cccccacctc ggcgcgcact gcggccagca cctcgagcag caggcgcgcc cggttttcca
     1561 cgctgccgcc atagcggtcg gtgcgggtgt tggtgatcgg cgagaggaag gaggaaagca
     1621 gatagccgtg gccggcgtgg aactctatgc catcgaagcc ggcgatctgc gcgcgccggg
     1681 cggcctggac gaactggtcg atgaccacgg cgatatcggc ctcgtccatc accttgatgg
     1741 ccggcgcctt catgccggcc agcccggcca gctcttccat caggaaataa tcggtgaaac
     1801 tgcccttggg cgcgggcggc accgccggcc cccacagcgg atggccccag cggccggcgg
     1861 cataggccgc ggccagcccg ccatggtgca gctgggcggc aatgcgcgcg ccatgggcat
     1921 gcacggccgc gcacagccgc gccaggccgg ggatgaagcg atcatcggaa atggcggtct
     1981 gctggcgggt gacactgccc accggccagg caacgccggt aacgccggtg atgatcagcc
     2041 ccgccccgcc gcgcgcctgt tcctcgtgat aggccagcaa ttgggcaccc acgctgccat
     2101 cgtcctcggc caggctaacg cccatggcgg tgacgaggat gcggttcttc gtctccattg
     2161 cgccaatgcg gcccggcgac aggagatacg gatagcggtt cgagatcatg cgaaggctcc
     2221 atggcgcggg gcgtttggct tgcggcctat gcgtggaatg gggatctgca gtttacgaaa
     2281 caggcaatgg gccttccata aggcgaggcc gcgccgcccg accgtggctg atgtcggatg
     2341 gcgcggcctc gggcagtctt cccgaactgc cctgtcgctt gtggccggcc tgtggcctca
     2401 gagcttcacg ccaagcgaca caccataggt gcgcggcagt tcgtaatagc cgccggcata
     2461 gcccagcgcg ccaaaatcga tgccgcgggt cagcgcgtgc tcgtcggtca ggttcttcac
     2521 ccagaacttc agctgcgcct cggccgtgcc caccttgaag cggtctacac cgaattgcag
     2581 gtctaccagg ttgcgcgcat cgcccttgat cgcattgttg aaaggcgagg tgagcacctg
     2641 cgagaagctg tattggccat cctcatgcgt atagccgatg cggccgaaca ggcgggcatc
     2701 gccccggccc agcgggtaat gcgcatcgag cgcgatattg gccgtgagcg gcgaggaata
     2761 ggtgcccact gccagcgagg ccacgttcac cgtggcgccg gtggtcgaca ggcccgagtt
     2821 gaactcgatg aacttggtcg agacatagcc gatgctgcca tcgatcgaga cgtagcgcga
     2881 gaggatcgcc tgcgcatcgg cctcgatgcc gtcgtaatgc acgcggccgg cgttctgcac
     2941 gcgcgagaca aaggtgccgc ccgaggccga caccaccggc acggtcactt ccaggttctc
     3001 gtagacgttg tgataggccg ccacgttcag ccgcaggtgc cggtcgagca gttcggtctt
     3061 caggcccacc tcgtaggagg tcacgctttc cgggttgaag tgcgccagcg tgttggtgcc
     3121 ggtgatgctg ggatcggcgg cgttgaaccc gcccgagcga tagcctgtgg ctacccgcgc
     3181 atagccggtg atgcccttgg caaaatcata gcccagcatc gcgttccagg tgaagcggct
     3241 gaagttggca ttgccggttt cgggcgtggt gtcgggggtg gagccgttct ggaaacggag
     3301 catgctcttc tggtcccagg tgtagcgccc gcccagggtg aggcgccagc ggccatggtg
     3361 cccgccggga tagaaggtgg cctgcgcata ggccgcctcg ctttgcgagg tggtctgata
     3421 gttcagcacg gtcagcgtct gcaccaggcg gtattgcgcc ggattggcgg cggcaaagct
     3481 ggaagccagg gcgccgaaat tggccgcggt gaacaccgag gcgttggtgt cgagcacata
     3541 gccgctgttc tgcggattgt attcgatgcc cttttcccag aaatagaagc cgcccacgac
     3601 ccagtcgaac cggtcgctgc tgcccgagag ttccagctcc tggctgagct ggcgctggct
     3661 gcgctggtcg gccgaggtga acaggttctg ggtgatggtg ggcacggcgc tggctgcgat
     3721 cgccgcgtca taggcggtgg ggatcgaggg gatgtattgc agcagcgcgg ccggcatgcc
     3781 gttgaacagc gaggccgagg tgtattgcgc gccgctgaag gcgccgatgc cgtcgagatc
     3841 ggccacgttg gtgttgtgcc agatgcgata gccggtggtc gatttcagct tgaagctgcc
     3901 gaaatcattt tccacctgca ggttgtggcc ccacatcttg tcgttggaat agttggtgat
     3961 gtcgttgcac acggtgttgc gccaggcgcg ctggggcgtg gccagcgccg cgcaggccgg
     4021 attggcaaag gtgaccccgg ccagatattg cgacaccggc gcctgctgct gcaccgtgac
     4081 ctgctggccg ttcaccgaaa tgggcgcgtt ataggcgccg ttcgaggtgt tggtgagctg
     4141 gaagttcatc gggctgccga tggtctgcga catgtcgaag atatactgga tggtgccgct
     4201 ggtgcccaga tgggcgcggg cggcagcgcg gaagccattg ctgcggaagg cgccgggatc
     4261 gcgcgagcgg tcgggctggt cgagattggt gacgacgcct tcgcgttcgc ggtgaacgaa
     4321 gaggaaggag gtgtcgatgc cggcgatctc gcccggatcg accgagatct tgccgttcca
     4381 ggcgttatag ttgccatagc ccacttcggc ggtcaggcgg aaggtgtctg agggcttgcg
     4441 cgagacgaag gagatggcgc cgccggtggt gttgcggcca aacagcgtgc cctggggccc
     4501 gcgcagcact tccacccgct cgatatcggc cacatcgagg ccggcttcgg ccgcgcgggc
     4561 aatgtagatg ccatcgacat agaggccgtt ggccgcatcc aggccaaagg tttcggacga
     4621 gcccgaggag atgccgcgaa tcgacatcac cccggcgctg ttgctggtgg tgcccggcgc
     4681 aatggtgacg ttgggggcaa tgcccgacag gtcgcgcgcg tcggacacgc cgatcttggc
     4741 gatcttctcg gcgctgatcg cgctgaccgc gaccggcacg acctgcaggt tctccttgcg
     4801 cttctgcgcg gtgacgacga tctcgccgag ctggttgtcg gccggcttca catggtcttg
     4861 cgcggtggtg tcggccttgg cgctggccgg gtttgccagc cccagcgcca tagcgccggc
     4921 gcccagcaac aggcgatatc ggtggacatg catcatagcc actcccctcc tttgattggt
     4981 tttgtggcca ttttgatgaa tgcgtagaaa atgtcaatca atggtacgca aaattatcat
     5041 catgtggaat tttgaaagat atttgtggcg ttgtgtggga aaaattccgc tgttttcctg
     5101 ctgtacgggc ggtcagagca gcgcccgcag cgctgcgctg gcggcgatcg ccaccaggca
     5161 tgccagcccg atctgccggt agtgccgccc gcccgacagg cggaacaggg attcgcccgc
     5221 cagcgtgccg gccatcatgg cgggcaggca caggcccgcc agcagcagca ccggcgcgcc
     5281 cagccctgcc acccacagat tgccggccag cgccagcccg ccggtaacca ggaagatcag
     5341 gatcatcgag gcgcgggcgg cggccggcgg cgtgcccacg gccaggtaat aggccagcac
     5401 cggcggcccc ggcatggcgc aaagccccga cagcaccccc gccagcagcc cgaacggcag
     5461 gatgaagccg ggcccgtgcg ggcgcgtgtg gcgccagccg ccggccagca ccagcgtggc
     5521 gccggccgac accccggcga tgcacagccg cgccgcggca aggccgatat gatggaggat
     5581 ccacacgccc agcggcgttg tcagcatggc ggccaggctc agccagcggg ttgatccggg
     5641 ctcatgcgcg gccagcgtct ggcgcaggcc gacaaggctg gaggccacct gcagcaacag
     5701 caccaccggc accagctcgc ccggcgccag caccagcgaa agcagcggcg tggccgccag
     5761 cccgaagccg aagccggtga agccgcgcag ggcgccggca aggccggtgg ccagcaggca
     5821 gccggccagc acggccccgc cgggcagatc agcgcccagg gcaggcagca gcatggctca
     5881 ggccgcggcg aagacgatgg tcttcttgcg ggtgaaatgc tccagcatgg cttccagcga
     5941 agcctccttg cccaggcccg atttgccaaa gccgccatag ctgagattgg gctggatggt
     6001 gaggttctgg ttcacctgca catagccggc atcgaggcgc cgggtggcat cgagcgcggc
     6061 cgacaggtcg ttggtccaga tgctggcggc aaggccgaaa tcgctgtcgt tggccgccgc
     6121 gatcacttcc tcgtaatcgc gccagggctg gatgcaggcg acagggccga agatctcctc
     6181 gcgcagcagg gggctgtctt cgggcaggcc ggtgaacagg gtgggcaggg caaacagcgc
     6241 cggatcgagc cccgcgcccg ccggcggctg gccgcattcg atcacccggg cgccaggggt
     6301 ctgccggccg atctcgatgt agcgggcaat ggtggccatc tgctcgggcg agatgatggt
     6361 gccgatatcg gtcgcctcgt ccagcggatc gccgatgcgc agcgcctgca gcgcttcctc
     6421 cagcgcggcg acgaagggcg catggagcgc ttcatgcaca tagatgcggc tggccgcggt
     6481 gcagctttgc ccctggcgcg tgaagcgcat gccggcgatg gcgccggcca cggccttggc
     6541 aagatcggca tcggccatca cgatcatcgg gctcttgccg cccagttcca gcgagaccgg
     6601 cacgatcttg cgcgcggcgg tggcatagat cgtctcgccc accgcttgcg agccggtgaa
     6661 ggtgaccttg ccgaccagcg gatgctccac cagcggctgg ccgcacgccg gcccgaagcc
     6721 gctgacgaca ttgagcacgc cggctggcag cacctcggcc atcagccggg ccgcttccag
     6781 cgtggcaaag ggcgcttcct cggcggattt cactaccacc gcattgcccg ccaccagcgc
     6841 cggggcggcc ttcagcatca tcagcaccag cggcacgttc cacggcagga tggcggccac
     6901 cacgccgatc ggctcacgcg tggtgaggca cagcagatcg gggcggaagg gcagcgtctc
     6961 gcccttcagt tcagagccga gcccggcgta gaaggccagg atatcggcgg cggtgaggat
     7021 ttcgggccgg cattcggtgc ggatcgcctt gccggtttcc agcgccagca gccgggccag
     7081 cggttcggtt tgcgccagca ggcggcgggc ggcctctccc accagccggc cgcgttcacg
     7141 cgcgggcagg gcggcccagc cgggctgggc ggccgcggcc gcttgcaccg cctgatcgac
     7201 atcggccgcc gtgcccgccg cggcatggcc cagcaccgcg ccggtggccg ggttgatcac
     7261 cgcgaacaga tcgccggcgg gcgccaccag ctgcccgttg atgaggtgcc ctgcgcggat
     7321 tggcgctgtg ccatgggccg ccatgtcagg ggtgtcggaa agaatatgcg ccatagtgcc
     7381 tcctgcggtt gccgggcagt taggcgtggg gccaggggcc gcgataggac cagttgccgc
     7441 cgaccgttgg gccagccgct caccccatct ggcgcgcgat gatctcgcca agcagggcaa
     7501 tgccggcggc aatgcgcctt tcggggataa ccgaatagcc aaggcggata tgccggccgg
     7561 ggctgtcggg ggtggcaaag aagatgtcgc ccggctcgaa cagcaggcct tctgcgcgtg
     7621 cggcctgggc ggcggcctgg gcatcaaagc ccttggggcc gcgcagccac aggcaggagc
     7681 cgcccgtggg cggcacgaaa tccacccccg gcagcgccga gagcgctgcc accagctggc
     7741 cggcgcgggc gcgataggtg gcggtgagcc ggcgcaccag cgattcataa tggccatagg
     7801 aaaggaacag cgccaccgag cgctggttgt tgagcggcgg atggcgcacc atcagccggc
     7861 gcaaggcgcg caattcgcgg atcagcgcgg catcggccac cacgaagccc agcctgaggc
     7921 ccggcgccag cagcttcgac aggctgccga catagatcac ctggccggtg cgatcgaggc
     7981 ttttgagagc ggggcggtgg gcggcctcga agctggtttc gctgtcgtaa tcatcctcga
     8041 tcagcaccgt gctgctgcgc tgcaccgcct cgatcagcgc catgcgccgt tcgtagggca
     8101 tggtaacggt ggtggggcac tggtggctgg gggtgacatg cacatagtcg caggccagca
     8161 ggtcggcatc gggttgcagg ccggcggcat ccaccggcag gcggcacagg tgccttgtgc
     8221 gcatggcggc gatgttgcgc atgtcgggat agcccggctc ttccaggccc acccgcgttt
     8281 ccgggccgaa cagggcttct gccagcagat agagcgcctg ctgcgcgccc agtgtcacca
     8341 tgatctcgtc gcgctcggcc cagatgcccc ggcgcggcag gatgcggctg cgcagctgct
     8401 cgatcagcag ggggtcgtcc tcgtcgatct tgtcgggcgc ccagtcgcgc agttccagca
     8461 cgctcagcgc ctggcggcag cactggcgcc attcggcgat gggaaaggcg ccggcatcca
     8521 cctgcccgta aaggaagggg aagcgatagg tgtgccagtt cgatggcttc tcgatatggc
     8581 gcagcgccgc cgcgcgtgag cggacgcggg cgcccagatc gatccgctgg gccggcgcgc
     8641 gcgcgggggc ctggctgacc cagccggcag cggcattgcg ggcaacgaca aagccgctgc
     8701 gctcgcgcga ttcgagaaag ccgtcgtcga ccagctgctg gtaggagagg atgacggtgt
     8761 tgcgcgagac cgccagttcg ctcgccagcc cccggctgga aggcatcgcc gtgcccggcg
     8821 ccagcgtgcc cgacaggatg gcatcgacca cgcgggtgcg cagctgcacc tgcagcgggc
     8881 gcgcatcgct ggcatcgatg gccagcaggc gctgccacag gatcgcgccg cgtttcctca
     8941 cattagccac catacgcctt gcaaaccctg tctgttgttt tctgccggtg ggccccatcg
     9001 ggcgggcccc tgctggccgg cttgtcccat gcgattgggg gaaactggca ctatgtgcgg
     9061 ccagcaaaaa gaggatgctt gaaaaccatg gaaacacaag ccccccctcc cgcgcttcgg
     9121 cccgatctcg cccatcactg gatgcccttc accgccaacc gggatttccg cgaggcgccc
     9181 aagatgatcc tgcgcgggca gggtgtggtc tattatggcg agggggggca gccgatcctc
     9241 gatggctgtt cgggcctgtt cacttgccct gccggccaca gccgccccga gatcgccgag
     9301 gccgtgcgcg accagctgct gacgctggat tatgccccca gcttcacccg cggccatccg
     9361 ctttccttcc gcgcggcaga gcgcatcgcc gcgctgacgc cggccgggct ggaccgcatc
     9421 ttctttgcca attcgggttc ggaagcggtg gaaaccgcgc tgaaaatctg cctggcctat
     9481 caccgcgccc ggggcgaggg cacgcgcagc ctctttgtct cgcgggagcg ggcctatcat
     9541 ggcgtcaatt tcggcggtgt ggcgctgtcg ggcatggtgc ccaaccggcg cgcctttggc
     9601 ggggcggggc cgcaggtggt gcatatgcgg cacacctggc tggaggagaa ccgcttcacc
     9661 cccggccagc ccgttcatgg cgccgaactg gccgaggacc ttgcccgctt cgtggcgctg
     9721 cacggggccg agaacatcgc ggcctgcgtg gtcgagccga tcgccggatc aaccggcgtg
     9781 ctggtgccgc cgcccggcta tctggagcgg ctgcgcgaga tctgcacgca aaacggcatt
     9841 ctccttgtgt tcgacgaggt gatcaccggc tttggccgca ccggccatgc ctttgccgcg
     9901 caggcctttg gcgtggtgcc cgatctcatc accatggcca aggcgatcac caatggcgcc
     9961 cagccgatgg cggcggtggc ggtgagccgg catgtgcatg acacgatcat ggaggccgcg
    10021 cccgaggggc aggtggaatt cttccacggc tacaccttct cggcccatcc ggcggcctgc
    10081 gcggccagcc tggcggcgct ggagatctat gagacagagc atctgttcga ccgggcgcgc
    10141 cagctctcgg cctatttcct cgatgcggtg atgacgctgg ccgacctgcc ggtggtgacc
    10201 gacatccgcg gctttggcat gctggccggg atcgacatgg cgccggcaga gcgcccgggc
    10261 ctgcgcggcc atgcggtgca aaagcgcctg ttcgatgccg gcctccatct caagaccacg
    10321 ggcgatgcgg ccctgctggc gccggccttc gtgaccgaga cggggcagat cgacgcgatg
    10381 gtggggatcg tgcgcgaggt gctgtcggcc ctgggctgaa ggcgcggccg gatcggctgc
    10441 gcaaaaccgg caataccccg ggcgcgcgag ggaacgcgcc cggagaacat gagcacaggg
    10501 atatgtaaaa acaagataaa atgaggatgc ccttctggcg ataatcacca aagggaggct
    10561 gacaatgcgt gttgtttcat cacacgaagt gcaatcacat caggcgcaac cacacgaacc
    10621 gcaaacgcgc agatctggaa agagggggat cttgctgtgc tgcgcggcca gctgggcgct
    10681 ggcgctgccc ggcatagcgc aggcccagca ggcccagcag gcccagggcg ggctgggcga
    10741 tatcgtggtg accgcccaga agcgcacgga aagcgcgcaa ggcgtgccgc tgtcgatcca
    10801 gagcctgggc gcccagaagc tggaggcgct gggcgcggcc aaattgcaga acatcgagat
    10861 ggccgcccct tccgtcagct ttggcgatgg cagcgagcag ggccgggcgg gcatccgcgg
    10921 ggtgatcgat tattcccgca atgccggcta tgacagccgc gtgggcattt atgtcgatgg
    10981 cgtgtattac agccgttcgt ggatgaacaa cctcaccctg ctgggggtgc agcaggtgga
    11041 tgtgctgcgc gggccgcagg gcaccttgtt cggcaagaac accgatgccg gcgccattgc
    11101 catcaccacc ttgcagccgg gccgccgcac agagggcgaa gtgcaggccg aagtgggcaa
    11161 tttcggctat tggaagctgg gcggccggct ggcagccccg gtgggcagcg atctggccat
    11221 gcaactggcc gccacccatg tggaaagccg cggctattat cacaacagct acaccagcca
    11281 gcgcaaccag ggcctgcatg gcgatgcggt ggcgggcaag ctgcgctatc agccgggcgc
    11341 gggcatcgat atcaccctgg ccggcgatta cacccgcgat cgcaacacca cccagcatta
    11401 caccgccccg ccggccgccg gcggcgaccc ctacactttc cgctcctact acaacgacca
    11461 tgccaatcgc gacatgggcg gcctgtcgct gagcgccagg gtgccgctgg gcggcggcta
    11521 tgatctcacc gcgatcaccg cctggcgcgg cggccagcag gatctggctt tcaacaacga
    11581 aaccggcacc acgccctatc tcaccgcgct gctcaaccag cacacgcgcc agttctcgca
    11641 ggaagcgcgc attgcctcgc cgcgccggcg cacttatgat tttgtggccg gcgtctatta
    11701 tttctgggcc aacaaccagg atgattatac cagcctgtgg ggttcgggcc tggcgcagct
    11761 gggttcggtc tatgggctgt atgccggggc cagcacgggg gcgcatgcct cggtcagcac
    11821 gcaatcggtg gccggtttcg tgcagggcag ctggcggccg ggccgggtga tcgaactgtt
    11881 tgccggcggc cggctgacct atgagcagaa gcggctgaac gatctgacca cccacgatcc
    11941 ctatggctat ttcctcgccg gcctgcatgg ctatgccgac agcctgagca acagcttctt
    12001 cacgccaagg gggggcgtga acctgcatct gacgccccat gtgctggtgt ttggcacggt
    12061 gtcgcgcggg ttcaagagcg gcggctggaa tgtggaaggc accagcctgg ctgcccaggc
    12121 cgccggcatc cgcttccgcc cggaaaaggt gacgagctat gaaatcggcc tcaaatccga
    12181 tttcctcgat cgccgggcgc gcttcaacct caccggcttt tacgagcgct ttaccgattt
    12241 ccaggtcttc accttcgtgc cgacaacgct ttatggcgtc acctcgctga ccagcagcct
    12301 caccaatgcc ggggcggtgt cgtcgcaagg gggcgaggtg gaactggcgg tgatcccggt
    12361 gcgcgggctc accgtttcgg ccaactacac ctatgatgtc agccggttcg atcgctatcc
    12421 gggcggcggc ggcacggtgg cgggcgtgcc gctcgatgcc catggcgtgc agacacccta
    12481 tgcgccgcgc cacaagctct atgccgcgct ggattatgcc ggccagctga gcccggcggt
    12541 cggcatcacg gcccatttcg gctatagcgc gcaatcgtcg gagaatttcg atcccaaggt
    12601 gatcaacccg gcctatggcc gctattactt catccagggc tatgatctgg ccgatgcgcg
    12661 ccttggcctt gccggcaccc acagccgctg gagcgcggcg ctgtggtcga agaacctgtt
    12721 cgaccggcgc tatgtgctgt ttgccaaccg caccgcgctg ctgcaggcgc cggcggtgat
    12781 gtatggcgag ccgcgcagct tcgggctgac ggtgggctat cggttctgac aggcggggca
    12841 ggggtgggcc cgggcggtgt gccgggcccg cttcagctgc cgcacagcgc tgcccaggcg
    12901 ctttccagca ccgccggcgc aaagggcccg gcagggccaa ttgccacgat ccgcgcggcc
    12961 ggatcggtgc cggccggcgc tgcctcgatg gccacctgcc ggcccacgcg gtggcaggca
    13021 taggcgctgc cggccggatc gggggcaatg cctttcaccc ggtgcaccca ttccggcagg
    13081 gcagccagga aggcccgcat ggccgcagga ggaatggccc cgccggctct gcccgaccag
    13141 ctgcgatagc catggccagc cccggttggc ccgggcgcgc tgcgccagcc ggccgggccc
    13201 agcagcagat cgggcgccac ccggccctgg cgcgtggcca gccgcgccgc gcccggcgcc
    13261 agatcggcca gcagggcgca aagggcggcc agctcttcgg gggccaccag atcggccttg
    13321 ttgagcacca gcagcccggc cgcgccaatc tgccggcgca cggtggcgcc cgcccagcga
    13381 tcggccaggc gctggcggat gccggccgca tcaaccagcg ttgccaccgc atcgagccgc
    13441 agcagcggct ctgcctgcgc cagctgggcc agctgcaccg gatcggcaat gcccgaggct
    13501 tcgatcagca ggtgatcggg ccgggccggg cctgccagcg cgcgggccag cgcccccagc
    13561 agatcctgtc ccacctggca gcaggcgcag ccattggcga gggtgatcac ccctccctcg
    13621 cgcccggcga tcagatcgcc atcgatcggc agcgcgccaa aatcattgac cagcaccagc
    13681 aaccgccgcc cgccggcatg gcgcagcaga tggttgacgg tggtggtctt gccggcgccc
    13741 agatagccgg caaggatggt ggcgggcacg ggcacgggca cgggcacggg cggctcgcgc
    13801 atcaggccag cgcgccggtg ggctggccgg ccagcaccgt gccggcaatg ccgatctcgc
    13861 gcagggccat cggatgcacg gcggtggggt cctcgtcgag cacggtgaaa tcggcccatt
    13921 tgccgatctc gatcgagccg atggtgtcgt ccatatgcag cgtgtgggcc gcccccaggg
    13981 tgatcgcgcg caaggctgcc tccacgctga tgcgctgggc cgcgcccagc acgcgccctt
    14041 cctgcgtgcg ccggttgacc gcgcaccagg cagtgaacag cgggccgatg ggggtgaccg
    14101 gctcgtcgga atggatggcg aagggcacgc ccagttccag cgccgtggcg caggcattca
    14161 tccgctcggc gcggtcgggg ccgatggtca gctcgtaatg cttgtcgccg aagtaataga
    14221 ggtggttggc aaacaggttg caggccagcc ccagcgcctt catccggcgg aattgcgcgg
    14281 catcggccat ctggcaatgc tgcagcgtgt ggcggtggtc gaagcgcggg gtgcgggcca
    14341 gcgcggcagc aaaggcttcc agcgccagat cggtggccag atcgccattg gtgtggatgt
    14401 ggatctgcac gccggccgcg ttcagcatcg ccaccagctc gaacaattgc tcgggcgcga
    14461 tgttccacag gccgtgatcg gtgccgcgat aatagccggg ccagcgcaat tgcgcggtga
    14521 agccctggat cgagccatcg agcacgatct tcaccgcccc cagccgcagc ttgtcggaac
    14581 ccagcggcgc cagggccagc gcctcggcca ggatctcggc cggcgggcgg tggtgggcca
    14641 tcagcatcac ggccaggcgc agcggaaaat cgggccggtc gatgaaggcg cgcagggccg
    14701 gcacatcggc cgcggcaatc gtggcgccaa gatcggtggc ggtggtcacg ccggcgcggc
    14761 gcgccagttc ggcaaaggcg cgataggtcg gctcgccgct ttgcaggccg gccagcccca
    14821 gccgcatgaa caccggcccc atcgcggcca tctcgtgcaa ttcgccggtg ggctcgcccg
    14881 cggcatcctt gcacacgccg ggcacgcagg tggtgcgatc gtaggcggca agggccaggg
    14941 cggcggaatt gaccgtcatc agatggccgt tggaatgcag cacgatcacc ggccgcgtgg
    15001 ccgagacacg atcgagatgc tggcgcgaga ggcgctcgcc ggcaacgaac agcggatcaa
    15061 agccccagcc catcagcacg cccgcgcgcg cggctgtggc cgcgctcagg cgcgccacca
    15121 ccgcttccgt ctcgcccagc cccgcccagc ggcggccggc cggatcgatc cgctccatat
    15181 ggccaagata ggtgtccttc cagagatggc cggtcatcgc atgggcgtgg ccctcgacaa
    15241 agccgggcaa cagcaccttg tcggcaaagc gcgtgtcgag cagcgccggg ccccagccgg
    15301 ccaggctctc ggcggtgcca acgcccagca ccttgccctc gcgcacggcg acatgggtgg
    15361 cgcgcgggca gttgcgatcc atggtgagga tggcgcgggc ggaaaagagg gtgatcgggg
    15421 gcatggcggc tccgtaatgg gaggaagaac aggcttctag gcgaagcgct gcccgccaac
    15481 tggacccaga ggccgccaaa gcatggtgcc agcaggacaa accggctggc ggcatgggcg
    15541 ccatgggcta gcctgccggc cattctgagc aggagcatgg cgtgcgtttc aagatttttc
    15601 ccggttggct gctggtgggg gccgtgctgg tggcgctgat gctgagcggg gcgacggtga
    15661 tcgtcagctc cttcggcatt gtcaccgcgc acctgaccga ccgcttcggc tggagccagg
    15721 tgacgatggc cggcgccctt tcggtggcgc tgctggctac cacgctggcg gtgccgctgg
    15781 tggggctcac ggtggatcgc ttcggcaccc ggcgcacggc catcgccggt tcgctggcgt
    15841 ttgccgcggt gctggcatcg gccgggctga tggtgcgttc gctgccgggg ttctatggtt
    15901 tttacgcgct gctgggggtg gtgggggctt tcaccaatcc catcgtctat atcgccgccc
    15961 tgtcggcctg gttcgaccgg cagcgcggcg tggcgctggg gctggcggtg gcgggccagg
    16021 ggctgggcgg cgcggtgctg ccggtgggca tagagacgat cgcccgccag ggcggctggg
    16081 cgatggcctt tgccggcatg gcggtggccg tggtgctggt ggcggtgccg ctgatcctgg
    16141 cctttgtgca tgatcacccg gccgcgcgcg gctgcctgcc cgatcatggc cattccaggc
    16201 ctggtgggca ggccgggcct ggcgggcctg ccggagcggc cgcgccccat ggccttagcc
    16261 tggcagaggc gctgcgctgc cgcagcttct ggatcatcat ggcggtgctg gccctgctgg
    16321 ggctgacgac ctatgcctat tcgggccatc tggtggcgct gatgcgcgcg cgccatatcg
    16381 ccaccatcgg gcaggccgcg ctgcttgcct cggtgtcggg ggcaacgatg atcctcagcc
    16441 gggtggcctt tggctggctg ttcgatcgct ttgccctgcc gctggtgggg gcgggcggca
    16501 ttgccctttg cgccctggcg cagttcctgc tgctgcggct cgatcatttc gggccgatgg
    16561 tgctggtgat ggggatgttg ttcggcgcct cgatgggggc cgagaccgac ctgctgacgc
    16621 tgcttgtcgg ccgctatttc ggcacgcggg cgctgtcgcg catctacagc tggcagaatg
    16681 tggcgtttct ggccggggcg gcggtggggc cggtgctgtt ctcggcgctg atgcaggggc
    16741 tgggcggcgt ggagctgccg gtgttcgtga tgatggggct gacggtgctg gccgccgccc
    16801 tgctgctggg cctgcaccgc gagccccagc acgggcgggc ggcgcaggcg ctggcctgat
    16861 ccgcccgccg caagcgccgc ctagcgggcg gcgacgacga tgatttccac cttgtagccg
    16921 ggggcgaaga ggcgcgcttc gccggtggct ctggcgggcg ggtgggccgg gtccacccag
    16981 gcgtcccagg cggtgttcat cgcgtcgaaa tcggcgatgt cggccagcca cacggtggcc
    17041 gagaggatct tggccttgct gctgccgcag cgggccagca gcgcgtcgat ctcggccagt
    17101 gcctcgcggg tctggccggc ggcatcgctg gcgggatcgc cgatctggcc ggcgaggtag
    17161 atcgtgtcgt tatggatgac ggcctcgctc atgcgggggc cgggatcgat gcgctggatg
    17221 gtcatggctg gttctttcag tcgggcgcga tagcgcgcaa tgtcattcag gcgcgatagc
    17281 gcgcaatggc cagatccgca tgcgcgattt cgggcgggcg gccgcagatc acatcggcca
    17341 gcagccgggc tgatccgcag gccatcgtcc agcccagcgt gccatggccg gtgttgagga
    17401 agaggttgcc aagcggcgtg gcgccgatca ccggcgtgcc atccggggtc atcgggcgca
    17461 ggccggtcca gaattggggg tcggcgccgg catcggcggc ggtggggaac atgtcggcca
    17521 gcgatttgcg caaggtggcc agcgcggcgg tgcgcagctg ccggttgaag cccgagatct
    17581 cggcgatgcc gcccacgcgg atgcggctgc caagccgggt gatcgccacc ttgaaggtct
    17641 cgtccatcac ggtggattgc ggggcggcgg ccgcatcggc caccggcagg gtgagggaat
    17701 agcccttcac cggatagacc ggcaggccga tccccagcgg gcgcagcagg gcgggtgaga
    17761 agctgcccag cgccaccacg aaggcatccg ccactatccg cccggcgctg gtgtgggccg
    17821 cggtgatgcg cccgcctgcg gcctccagcg cgtgcaccgc cacgcccatc tgcatctcca
    17881 cgcccagcgc ccgggcctgt tcggccagcg cccgggtgaa gaggaagcag tcacccgttt
    17941 cgtcatgggg caggcgcagg ccgccggcga tgcgcgcgcg cgcgcctgcc agccccggct
    18001 cggcggcgat gcagccggct ctgtcgagca gttcgaaagg cacgccatag gaggatagca
    18061 cggccatgtc cttgccgatg gcatcgagct ggccttgcgt gcgcagcacc tgcagcgtgc
    18121 cctgctggcg gccgtcatag gcgatgccgg tctcctgccg cagcgcatcg aggcaatcgc
    18181 ggctgtattc ggccaggcgc accatgcgcg ccttgttggc ggcataggcg ggcgcgttgc
    18241 actggcccag catgcgcgcc acccaccccc aggcggccgg gctgaggccg ggccgcagga
    18301 tcagcggggc atggcgcatg gccagccatt tcagcgcctt cagcgggatg ccgggcgctg
    18361 cccagggcga ggcatagccg ggcgagatct cgccggcatt ggcaaagctg gtttccagcg
    18421 cgggggccgg ctggcggtcc accagcacca cctcgtggcc ggcgcgggcg agataatggg
    18481 cagaggtgac gccgatcacc ccggcgccca gaatggcgat cttcatggca tctgctccgt
    18541 ggcgagaggg gcggcgccca gatagcgccg cgcatagcgg ggccccaggc cggtgaggat
    18601 ttcataggcg atggtgccgg cctctgcggc cacctgcccg atcgggcggt tggggccgat
    18661 cagctcgacc agatcgccct cggccagggc atcgggcgca agggcggtga tgtcgacaag
    18721 ggtgctgtcc atcgagacgc ggccggcgat cggcaggatc tgcccgccaa aggccgcaaa
    18781 gccccggttg gaaaggctgc gcggccagcc atcggcatag cccaccgcca gcgctgccag
    18841 ccgggccggg gccggggcct gccagctgag gccatagccg gcgcccgcgc ccgccggcag
    18901 atcgcggatc tggatcaccc gcgcatccag ccgcaccacc gggcgcagcg ccggatcggg
    18961 gtggattgcc ggctgggcgc catagagggc caggcccggc cgggcgatat cgccgccaaa
    19021 ggccggcccc atcaggcagc cgccggaatt ggccaggctg cgggcaacgc cgggaaaatg
    19081 cgcggccgtg gcggcaaagc tggccagctg gcgggcattg gcggggtctt cgggcacatc
    19141 ggcgcaggcc aggtggctga tgaggcccgc cagcggcacg gcggcgcgaa aggcggtatc
    19201 cgtggcgagg agggcggcct ctgccgggct gacgcccagc cgggccatgc cgctgtcgac
    19261 ctggaggaag gcgggcagcg ggcggtgctg ggtggcggcc tctgcctgcc agcgccgggc
    19321 ctgtgccagc gtgttcaaca ccggccgcaa gccggcgcgc gcgcaggccc cttcggcgcc
    19381 gggcaccagc ccgttcagca cgcccagcgc ccctccttgc ggcagcaggt cggccagtgc
    19441 cagcgcctct tccagatggg cgacgcagaa cagccggcaa ccggccgccg ccagcgcccg
    19501 caccaccggc cgcgcgccca gcccataggc atcggccttg accacggcgg ccacctgcgc
    19561 tgccccgctg gccgcgccga tatggcgcca gttggccacc agcgccgcaa gatcgatggt
    19621 gagaagggcg ccggcgggcg gcgggggcaa ggccatctgc gtaactcctg tgctgcggca
    19681 cgaggctaca ggtggattat aggcatgtgt tggcaaaatg gcgcatgaac ttgcgtgaac
    19741 gccgccattc agacagatta tgcggcattt ctggtgaatg atgccgcaag gatagcccca
    19801 tgcccacgct cgacaaggcc gatcgcgaga tcctgcgcat cctgcaggtg gatgggcgca
    19861 tttcgaatca ggatctggcg cagaaggtgg gtctgtcgcc ctcggcctgc ctccggcggg
    19921 tgcggctgct ggaggggcgc gggatcatcc gtggctatgc cgcgcaggtc gccgcctcgg
    19981 aggagaccga ggccaccgtt gtcatcgtgc ggatcacgct ggagaagcag accgaggaat
    20041 atctgcgccg cttcgagcat gccctgcggc aatatcccga gatcaaggaa tgctatctga
    20101 tgacgggcga tgccgattac accatgcgcg tggccaccgg cagcgcggcc gattacgagc
    20161 atatccacac ccatatcctc tcgcgcctgc cgggcgtggc gcgcatccat tccagcgtgg
    20221 ccatgcgcaa tgtgctgggg gcgggccgcc ggcgctagaa ctccacgccg gcctgggcct
    20281 tgatgccgct gcggaacggg tgcttcacca gcgtcatctc ggtcaccagg tcggcctgtt
    20341 cgatcagctc gggcgcggca ttgcggccgg tcagcaccac atgggtcagc tccggctttt
    20401 cctcgcgcag gaaggccagc acctcgtcca gcggcagata gtcgtagcgc agggcgatgt
    20461 tgatctcgtc gagcagcacc atgcggatgg ccggatcgcg gatcagcgcc ttggcctttt
    20521 cccacccctg ccgggccgcg gcgatgtcac gcgcgcggtc ctgcgtttcc caggtgaagc
    20581 cctcgcccat ggcatagaac tggcacagat cggcaaaatg gctctcgatg aggcgccgct
    20641 cgccggtatc ccacgcgccc ttgatgaact gcaccacggc gcagggcatg ccatgggcca
    20701 cgcagcgcag gatcatgcca aagccggagg aggatttgcc cttgccggtg cccgtgtgga
    20761 cgatgaccag gcctttctcg ccggtcttgc cggccatgat ccggtcgcgt ccggccttct
    20821 tcttggccat tttggcggcg tggcgctgca gatcggcatc cggggcgaga ggggcggtgt
    20881 ctgcggaaat ggccggggct cccttggctg gcggcggggc gctttgcttg acatgcgccc
    20941 gcgcatgccg caatcgcctg tgtgctggtt cctgcctata aagcgatttg tcggcaggcg
    21001 aagagggaat gcaaccgggc cgccccaggc ggttcgcaat cgcagccgcc cccgcgaccg
    21061 taaccggacg agacaggccc cgatgccact ggccccttgt gggcgcggga aggcaggcct
    21121 gtcgctggtg ccgcaaggca tcgcagtccg cagccgggag acctgccagc aggagaagac
    21181 cggcagccgg ggtgtgctgc gcgaggcgtt gcgggcgggc cctggcggca tgcccggcct
    21241 tgttgcatgc ccccgcgcca gaagaggacg cccgggtatg gagaatgagg caaccctgtt
    21301 cgtatgcgcc acctgccggg cgggcgatcc gcaggccacg gggccgaaat cggccggtgc
    21361 gcggctggcc gcggcgctga tcgccgcgca tgatggcgcc ccgggccttg ccatccgccc
    21421 ggtggaatgc ctgtctgcct gcaacaatgg ctgcgccatc gcgctttcgc gccccggcgc
    21481 ctggacctat gtctatggcc ggctgggcga gggcgatgtg ccgcagatcc tgtcgggcgc
    21541 tgtggcctat gccgccagcg ccgatggcat cgtgccctgg cgcgaaaggc ccgatatctt
    21601 ccgcaagcaa tcgcttgccc gcatcccccc gctggaggcc tgatccatgt cgctcgaaaa
    21661 gtcgctcgaa aagatccccg tcaccatcgt caccggcttt ctgggcgccg gcaagaccac
    21721 gctgatccgc cacctgctgc agaacccggg cgggcggcgg ctggcggtgc tggtcaacga
    21781 atttggcacg gtgggcgtgg atggcgagat actgaaggcc tgcgccgacg agaactgccc
    21841 ggaaagcgcc attctggaac tggccaatgg ctgcatctgc tgcaccgtgg ccgatgattt
    21901 catccccacg gtggagcagt tgctggccct gccgcagcgg cccgatcata tcctgatcga
    21961 gacttcgggc ctggccctgc ccaagccgct gctgaaggcg ttcgactggc cggcgatccg
    22021 cagccgcatc acggtggatg gggtgattgc cctggctgat gccgaggcgg tggcggccgg
    22081 gcgctttgcc cccgatgtgg ccgcggtgga tgcccagcgc gccgccgacg acagcctcga
    22141 ccatgaaacg ccgctgtccg aagtgttcga ggaccagctg gcctgcgccg atatcgtgct
    22201 gctggccaag gccgatctgg tggacgaggc cgggctggcg gcggcccgcg cggtgatcgc
    22261 cgccgaggcg ccgcgcccgc tgccggtgct gccggttacc gatggcgcga tcgatccggc
    22321 gatcatcctt ggcctcaatg ccgcggccga ggacgacctt gccgcccgcc ccagccacca
    22381 cgatggcgag gacgagcacg agcatgagga tttcgacagc accattgtca gccttggcga
    22441 agtggccgat cccgaagcgc tggtggccgc cgtgcagcag ctggcgcgcg cgcagggcgt
    22501 gctgcgggtg aagggctttg tggcggtgca tggcaagccg atgcgccttt tgctgcaggc
    22561 cgtgggcgaa cgcgtgcgcc atcagtatga ccagccctgg ggcgcccgcc cgcgcaccac
    22621 ccagctggtg gtgatcgccg agcatgaccg gctggaccgg gccgcggtgc aggccgtgct
    22681 gggcggcgcg ctggcgccgg cctgacgcca tgcatgtcgt cttccgcgag cggcgtgggc
    22741 tggaggatac cgggatcgcc accgatctcg gccaaagccc tgcgccgctc gtggtgctgt
    22801 ccttttccga cagcgatctg ggtgcttttg ccgcgggctg gcatgcggcc ggcggccgcc
    22861 tgccggcgct gcgcctggcc aatctggccg cgcttaccca tccgctctcg gttgatacct
    22921 atatcgcgcg cacgttgcag ggtgcgcgtg cggtgctggt gcggctgatc ggcggcgcgg
    22981 gctattggcc ctatgggctg ggccagttgc aggatctggc gcggcggcag gggctggcgc
    23041 tggccgtgct gccggccgat ggccgggaag atggcgtgct cgacgggttt tccaccctgc
    23101 cggtgccggt gctgcgccgg ctggcggggc tgtgcgatgc gggcggggcg gcagcggccc
    23161 gcgcggcgct gggcgagctg gcgcgggcgg cgggcctgcc cgcgcccaag gcgccagagc
    23221 cacagccaca ggcgctgatg ctgcccgcat gcggctggta tctgccgggc gccggcgtgg
    23281 tggcggggcc gccgccgggc gcgggcccgc tggcggtggt cagcttctat cgcgcctatc
    23341 tcgccagtgc cgatatgggc gcggtggatg ccttgctggc ggcgctggca gagcggggct
    23401 ttcgcgccgc ggggctgttc gcgccatcgc tgaaggcgcc gggggcagtg gcgtggatca
    23461 ccggccagct ggcctgccac cggccggcgg cgatcgtcaa cgccacggcg ttttccgcgc
    23521 gggacgaggc cggcgcctcc ccgctcgacg ggccgggctg cccggtgttt caggtggcgc
    23581 tgtccactgc cgggcgcgag gtctgggccg cggcggaacg gggcctctcg ccggcagacc
    23641 tcgccatgca tgtggccctg cccgaggtgg acgggcggct gtttgccggc gttgcctcgt
    23701 tcaagcaggc gggccgggcc gatcccgcgc tcgaattcgc ccgcgtgtgc cacgaggccg
    23761 atccggcgct ggtggccgcc attgccgatc gggtggccgg ctggcaccgg ctggccagca
    23821 cgacgccggc cgcgcgccag ctggccttcg tgctctccac ctatcccggc cgggcggatc
    23881 agatggccca tgcggtgggg ctggatgcgc tggccagcac cgaggcgatg ctggcggcgc
    23941 tggccgccga gggctatgac gtggcgccgg gcccgccgct ggccggcgcc ttgcaggcag
    24001 aggaggtgag ctggccgctg gccgcctatc gcgccgcgct ggccggcctg ccgcaagccc
    24061 tgcgcgatga tctggcgcgc gcctgggggg agccggaaag cgatccagcc gttgccgatg
    24121 gcgccttccg cttcgcggtg gcgcgccggg gcgccatgct ggtggcgctg cagcccgagc
    24181 gcggccctgc cgcggcccgc gagggcgatt accatgatct ctcgcgcacg ccctgccatg
    24241 gctatgtggc gttctatctg tggctgcgcg cgcaaggggt gcaggcgctg gtgcatgtgg
    24301 gcgcgcatgg cacgctggaa tggctgccgg gcaaggcggt ggcgttgtcg gcggcatgct
    24361 ggccgcaggc gctgatcggc gccctgccgg tgatctatcc cttcatcgtc aacgatccgg
    24421 gcgaggcggc gcaggccaag cggcggctgg gcgcggtcac gctgggccat gtgccgccgc
    24481 cgcagggccg cgccgaagtg ccgccggcgc tgctgcggct ggagcggctg ctggatgaat
    24541 attccaccgc cgaggggctg gatccggccc ggcgcgaccg cctgatcggc gccatccgcg
    24601 cagaggcgca ggccagcggc gtggaggcag accttggtct tgccgccggg gccagcgccg
    24661 ccgaggcgat ggtgcggatc gaccgtttcg tgtgcgatat caaggaaagc cagtttggcg
    24721 atggccttca catctatggc gcggccgcgg gcgagatggc tggcctgctg cgggcgctgg
    24781 atggccggcg ggtggcggca ggccctgccg gatcgccggc gcgcggacgc caggatgtgc
    24841 tgcccaccgg gcgcaacctc tacaccatcg atccgcgcgc ggtgcccagc cgggtggccc
    24901 atacgcaggg ggtgaagctg gccgaggaac tgctgcgccg ccacttgcag gatcacggcg
    24961 actggccgcg cgggctggtg gtggatctgt ggggctctgc cagcatgcgc acggcgggcg
    25021 aggaatttgc catggcgctg catctggccg gccttgcgcc gcgctgggat gcgggcagcg
    25081 agcgggtgag cggcttcgag atcctgccgc ccgccctgct gggccggccg cgcatcgatg
    25141 tcaccctgcg cgtctcgggc ctgttccgcg atgtgtttcc cggccttgcc cagctgttcg
    25201 aggcggcctc cgccgcgctg gccgagcgct gcgaggaggg gcccggggag aacccctatc
    25261 tggcccgcgc gccgcgcgtg tttggcccgc agccggggca atatgggctg ggcatggccg
    25321 atccggccag ccatctgggc gatgcggcgc ggctggcggc gggcgaggcg tggctgtcgg
    25381 ccagcgcctg gcggatcgat gcccagggcg cgatccacca cgacaggcag gcgctggcca
    25441 cccggctggc cggcgccgat gcctttgccc atgtgcagga cctgcccgag acagacctcc
    25501 tgatggcgcg cgactatgcc gcgcacgagg ccggctttgc cgccgccaag gccgcgctgg
    25561 ggcaggcagc gccggcgctc tatcacctgg atgccacgcg ccccggtgcc cctgtcgccc
    25621 gcagccaggc cgaggagatt gcccgggtgg tgcgcgcgcg ggcggccaac ccggcctggg
    25681 ccagcggcat gatgcgccat ggctggcggg gcgcggccga aatcgccgcc acgctcgaca
    25741 atctggcggg ttttgcccag ctggccggcc aggtcgggcc gcatctgttc gatctctatt
    25801 acgaggcgac gctggcgcgc gcggatctgg tcgatttcat ggcgcgggaa aacccgcagg
    25861 cgctggccga gctgcgggcg cgctttgccg cgctggtggc cgcggggctg tggaccacgc
    25921 ggcgcaattc catcgccgcg gcgctggcag gcggggcggc atgagcgcgc cggcgatccg
    25981 tggctggtgc ccgggggcgc tgcggccgat ggcctcgggc gatgggctgg tagtgcgcct
    26041 gcgcctgccg gccgggcgga tgaccgggcc gcaggcacgc gccatcgccg atctggcgcg
    26101 gcgcttcggc aatgggctga tcgatgtgtc ggcgcgggcc aacctccaat tgcggggcgt
    26161 gcagcccgat gggcatccgc cgctgctggc ggcgctggcc gagctggggc tggtggatgc
    26221 cgatgctggc gccgaaatgc ggcgcaacgt gctgctggcg ccgttctggc aggcgggcga
    26281 tggcgcgggc gagctggcgc tggcgctggg cgcggcgctg gcgctgggcg cggcgctggc
    26341 gggggcgcag gatctggcgc tgccgggcaa gtttggtttt gcggtggatg gcggggcgct
    26401 gccggtgctg cgcggcgctt cggccgatat ccggctggag cgggccgcag gcgggcgctg
    26461 gctggtctat gccgatggcg cggggcttgg gcaggtggtg gcaggggcag aggcggtggc
    26521 ggccgcgctg gcgctggcgc gctggtttct ggccaccggc ggtgtggaac aggggcgggg
    26581 gcgaatggcg gcgcatgtgc ggcgctgcgg cgccctgccg gatgaggcca acagcgcggt
    26641 gccgcaggcc cccgcctttg cccccgtgcc gggcccggtg tccgcaggtg tgctggtggg
    26701 cgtggcattc gggcggttga gcgccgaggt gctggccgcg ctggccgaag ccgggccgat
    26761 ccgcctgacc ccctggcgga tgctgctgct ggaagggtgc cgccaggcgc cggcgctggc
    26821 cggccttgtc accctggcgg atgatccgcg gctgcgcctg gcggtttgcc ccggcgcgcc
    26881 gcaatgcacc caggcgctgg ccgaagtgcg cgatctggcg ctggcgctgg cgccgcatgt
    26941 gccggcgggg caggtgctgc atgtctcggc ctgtgccaag ggctgcgcgc atcctgcgcc
    27001 ggccgatctg gtgctggccg cgcgccccgg cggctttggc ctcgcccgcg gcgccaccgc
    27061 cgccacagcg gccagctgcc ccccgatcgc ccgcgcggcg ttgttgtctc accccgaatt
    27121 gctgcaggcg gaatagtgat gccccatgtg tatgaacagg atggcgcggc catctatcgc
    27181 caatccttcg ccaccatccg cgccgaggcc gatctggcgc gcttttcacc caccgaggag
    27241 caggtggtgg tgcgcatgat ccatgccacg ggcctggtcg ggctggaggc gcaggcggcc
    27301 ttcacccccg gcatggccga ggcggcgcgc ggggccctgg ccgcgggcgc tgtgatcctg
    27361 tgcgatgcgc gcatggtatc ggaaggggtc acccgcgcgc ggctgccggc cgataaccgg
    27421 gtgatctgca cgctgcacga tcccgccgtg ccggagatgg cgcgggcgat gggcaacacc
    27481 cgctctgccg ccgcgctgga gctgtggcgg ccgcatctgg ccggcagcat cgtggccatc
    27541 ggcaatgccc ccaccgcgct gttccatctg ctgaacatgc tggaagaccc ggcctgcccg
    27601 cgcccggcgg cgatcatcgg ctgcccggtg ggctttgtgg gggcgatgga atcgaaacag
    27661 gcgctgatgg ccgcgccgcc ctgcccggca atggcggtgc acggccggct gggcggttcg
    27721 gcgatgacgg tggcggcgat caacgcgctg gccacggaga aggaatgatg ggcaagatca
    27781 tctgcgtggg gctggggccg ggcgaccccg acctgatgag cgtcagggcc gaccggctgg
    27841 tgcgcgcggc ggcccatgtc gcctatttcc gcaaggccgg gcgagcgggg caggcgcgcc
    27901 gcatcgtggc aggcatgctg gcggcagatg tggcggaata tccgatggaa tatcccgtca
    27961 ccaccgaaat cgccttcgac agcgctgcct ataacgagct gctggccgcc ttctacgacg
    28021 attgggcgca gcgcctggcc gcccttgcaa gcgatgccga tgtggtggtg ctgtgcgagg
    28081 gcgatccgtt cttctatggc tccttcatgc atttgcatgc gcggctgcag gggcaggtgg
    28141 cgatcgaggt ggtgccgggc atcaccggca tgaccggctg ctggcacgcc accggcctgc
    28201 cgatcacctg gggcgatgat gtgctgagcg tgctgatggg caccctgccc gaggccgagt
    28261 tggtgcgcca catgcgccag gccgatgcgc tggcgatcat gaagaccggg cgcaacctgc
    28321 cccgcgtgcg ccgggcgctg gcggcggccg gccggctgga ggatgcctgg ctggtggagc
    28381 ggggcaccat gcccggccag cgcgtgctgc gcctggccga ggcagaccct gccgattgcc
    28441 cctattttgc catcgtgctg gtgcatgggc aggggcgccg gccgcaggcc gtggccggcg
    28501 cggcggtgca ggagctgccg gcatgagcgg ctctgtggtg gtggccgggc tggggccggg
    28561 cgatggcgcg ctgatcacgc ccgaggtgac gacagcgctg gcccgcgcca ccgatatcct
    28621 cggctatatc ccctatgtgg cgcgcattgc cccccggccg ggcctgaccc tgcaccccag
    28681 cgacaaccgc gaggagctgg agcgggccgc ccatgcgctg gatctggccg ccgccgggcg
    28741 ccatgcggtg atcgtctctt cgggcgatcc gggcgtgttc gccatggcct ctgccctgtt
    28801 cgaggcgctg gaagagcggc ccgcctgcca cgatctgccg atcgccatcc tgcccggcat
    28861 caccgccatg ctggccgctg ccgctcgcgc cggcgcgccg ctggggcatg atttctgcgc
    28921 catcaacctg tcggacaacc tcaagccctt cgcgctggtg gaacaccggc tgcgcatggc
    28981 cgcgcggggc gatttcgcca tggccttcta caacccgcgc agccgcagcc gcccgcacca
    29041 gtttgcccgc gtgctggaga tcctgcgcga ggagctggcg ggcgagcggc tgatcctttt
    29101 tgcccgcgcc gtgtgcacgg ccgaggagga gatccgcacc accaccctgg cagaggcccg
    29161 ccccgagatg gccgacatgc gcacgctggt gctggtgggc aatgccgcca cgcgccaggt
    29221 gggccgctgg gtctacacgc caaggtcggc cgaatgagcc agccagccca gcaccgcgtt
    29281 cacctcgccc agcacccggc gggggggcag ctgcggccgc tcgaccagca gcaccggcag
    29341 gcccagcgca cgggccgccg ccagcttggc cgaggccccc gcgccgccgg cattcttggc
    29401 cacgacatgg gtgatcgcat ggcggcgcag cagggcgata tcgccctcca cgccgaaggg
    29461 cccgcggtcg atcaccacct cggcatgggg cagcggcagg ggcgctgcgg gcggatcgac
    29521 cagccgcagc aggtacacat gctgcggcgc tgcggcaaac acgtccagct gctgcttgcc
    29581 gatggcgagg aacacccgcg ccggcgcttg cggcagggcg gcgcaggcgg cggccatatc
    29641 ggccaccggc tgccagcgat cgccgggg
//
LOCUS       DASRYE010000236         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_28499,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000236 DASRYE010000000
VERSION     DASRYE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28499"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1519
                     /locus_tag="VFF98_12220"
     CDS             71..1519
                     /locus_tag="VFF98_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZU64441.1"
                     /translation="MTEPTGENPLTDSPVAAPSPARPRPPVHGDLTQGPILKTLLLFS
                     IPTLISNLLQTLNGTINAIWVGRLIGNSALAATANANLVMFLLFAAVFGFGMATTVRV
                     GHAFGAGDVVGARRVIGTGLGFCLMVSAVVGIAGSLLARPLLHALATPAASLEEALAY
                     LRVVFITFPLATLSLVVSMGLRGAGDAKTPLYAMILTVLVDAALNPLLIRGAGPVPGL
                     GIAGSAMATAFANALGSAFMIARAYARALPLRLGRGELRYLLPAREELGFVLAKGLPM
                     GAQMLLISSAGLVMVGLVNREGMMMAAAYGASLQIWNYLQMPSFAISSAVSAMVAQNV
                     GAGNHLRVGRITRAGVFSNIAFTTAMSSLIVLGAHPLLVLFLGSGSPAVPLASHMQLI
                     VTWSFVLVGVMMILTGTMRAYGSVIVPLVVMGVSMYPARLGFYFLAYPHIGAEALWWA
                     YLAGSVVAVGLTLAAYAGGRWRGHGKEAQPAR"
     gene            complement(1524..3158)
                     /locus_tag="VFF98_12225"
     CDS             complement(1524..3158)
                     /locus_tag="VFF98_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HZU64442.1"
                     /translation="MTVMEPLNPAAGNGARVRLGEVLEVSGSGSLIAVDPAAQAHFAG
                     DADPAVALAGQVGCPLKVWSGGRWLLATLRDLRRGPAGEIRAAIDFMGEGEGDPATGA
                     LHAFRRGVTHYPLPGAPVVPVTTADLAIAYAGDGSRATIAIGRVHPTSDIAASLYVDP
                     LLSKHFALLGSTGTGKSTALALILHRICDASPHGHVLMVDPHGEYGAAFRDTGMILDV
                     SNLQLPYWLMNFEEHCEILLSSRGDARQQEAEILAKCLHLARSRNRLAEQMRITVDSP
                     IPYLLSDLSTILSNEMGKLDKATTTAPYMRIKTRLDELRADPRYQFLFSGMLVGDTMV
                     ETISRLFRMPADGRPISIIDVSGVPSEITATVVALVARLVFDFAIWSRSEATRPILLV
                     CEEAHRYVPSERNADGSSVGRILSRIAKEGRKYGVALGLVTQRPSDLAEGVLSQCGTI
                     LAMRLNNERDQAFVKAAMPEGARGFLDVIPALRNREAIVCGEGVPIPVRLAFDDLEAH
                     RRPASDDPSFSALWATPGGEQALVARTVQRWRSHGK"
     gene            complement(3250..4005)
                     /locus_tag="VFF98_12230"
     CDS             complement(3250..4005)
                     /locus_tag="VFF98_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HZU64443.1"
                     /translation="MKRAALAAIAGFALMGAGEPLLVPDVSQGEVQVRQGFTGTELLL
                     FGAILTPEGARAARDYDVVVVLEGPARPMVLREKRKMAGIWVNADSITLRSVPSFYAV
                     ASSRPLRDIVDERTAAIYELGLGSLQLSPAEAVDEARQNHFAAGLVEQMGRKGLYRQD
                     EHAVEITGQVLYKARIQLPSSVQTGTYTAETFAIRKGRVVASALAHVEVKKIGFEKAV
                     ADHARHDGLLYGLLAVAVSVAMGWVAGRLFVRG"
     gene            complement(4002..>4512)
                     /locus_tag="VFF98_12235"
     CDS             complement(4002..>4512)
                     /locus_tag="VFF98_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU64444.1"
                     /translation="TLAATRRGETRPVARRRHHPLVAALPWRWRFYRSGLYISPLAPL
                     LLGLVTGILTMLMGIGGGFVLVPAMLYILGMGANVVVGTSLFQILFVTMATTMMHALT
                     THAVDIVLAVLLLFGSVSGAQLGTLFAMKASPARLRLILALIVLAVGARMALGLTYHP
                     DEIFTVSPL"
BASE COUNT          729 a         1537 c         1523 g          723 t
ORIGIN      
        1 cctgcctgca gcgcggctat cggttgacag atcgcttgca tatccacctt tccggcgaca
       61 ccagagggac atgacagaac cgaccggaga gaaccccctg accgacagcc ctgtggccgc
      121 gccttccccc gcccggcccc gcccgcccgt gcatggcgat ctgacgcagg ggccgatcct
      181 gaaaacgctg ctgctgtttt ccatccccac cctcatcagc aacctgttgc aaacgctgaa
      241 cggcacgatc aacgccatct gggtgggccg gctgatcggc aattcggcgc tggcggccac
      301 ggccaatgcc aacctcgtga tgttcctgct gtttgccgcg gtgttcggct ttggcatggc
      361 caccacggtg cgggtaggcc atgcctttgg cgcgggcgat gtggtgggcg cgcggcgggt
      421 aatcggcacc ggccttggct tttgcctgat ggtgtcggcg gtggtgggca ttgccggatc
      481 gctgctggcg cgcccgctgc tgcatgcgct ggcaacgccg gcggccagcc tggaagaggc
      541 gctggcctat ctgcgggtgg tgttcatcac ctttccgctg gccacgctgt cgctggtggt
      601 atccatgggg ctgcgcggcg cgggcgatgc caagacgccg ctctatgcga tgatcctcac
      661 cgtgctggtg gatgccgcgc tcaacccgct gctgatccgc ggggcagggc cggtgccggg
      721 gctgggcatt gccggctctg ccatggccac cgcctttgcc aatgcgctgg gctctgcctt
      781 catgatcgcg cgcgcctatg cccgcgccct gccgctcagg cttggccggg gggagctgcg
      841 ctatctgctg cccgcgcgcg aagagctggg cttcgtgctg gccaagggcc tgccgatggg
      901 cgcgcagatg ctgctgattt ccagcgcggg gctggtcatg gtggggctgg tcaaccgcga
      961 ggggatgatg atggcggcgg cctatggcgc ctcgctgcag atctggaact atctgcagat
     1021 gccctctttc gccatttcca gcgcggtttc agcgatggtg gcgcagaatg tgggggcggg
     1081 caaccatctg cgcgtgggcc gcatcacgcg ggcgggcgtc ttcagcaaca tcgccttcac
     1141 caccgccatg tccagcctca tcgtgctggg tgcgcatccg ctgctggtgc tgtttctggg
     1201 ctctggcagc ccggcggtgc cgctggccag ccacatgcag ctgatcgtca cctggtcctt
     1261 cgtgctggtg ggggtgatga tgatcctcac cggcaccatg cgcgcctatg gctcggtgat
     1321 cgtgccgctg gtggtgatgg gggtgtcgat gtatccggcg cggctgggtt tctatttcct
     1381 cgcctatccg catatcgggg cagaggcgct gtggtgggcc tatctggccg gatcggtggt
     1441 ggccgtgggc ttaacgctgg ccgcctatgc cggcgggcgc tggcgcggcc acgggaaaga
     1501 ggcccaaccg gcccgctgac ggattatttc ccgtggctgc gccagcgctg cacggtgcgc
     1561 gccaccagcg cctgctcgcc gccgggggtg gcccacaggg cgctgaagga gggatcgtcg
     1621 gaagccgggc ggcggtgggc ttccagatcg tcgaaggcca ggcgtaccgg gatcggcaca
     1681 ccctcgccgc acacgatcgc ctcgcggttg cgcagcgccg ggatcacatc gagaaagccg
     1741 cgcgcgcctt cgggcatggc ggctttcaca aaggcctggt cgcgctcgtt gttgaggcgc
     1801 atggccagga tcgtgccgca ttgcgagagc accccttcgg ccagatccga cggccgctgc
     1861 gtgaccagcc ccagcgccac gccatatttg cgcccttcct tggcgatgcg tgaaaggatc
     1921 cggcccaccg aggacccatc ggcattgcgc tcgctgggca catagcggtg ggcctcctcg
     1981 cacaccagaa ggatcggccg ggtggcctcg ctgcgcgacc agatggcaaa atcgaacacc
     2041 agccgcgcca ccagcgccac caccgtggcc gtgatctccg atggcacgcc cgagacatcg
     2101 atgatcgaga tcggccgccc atcggccggc atgcggaaca ggcggctgat cgtctccacc
     2161 atcgtatcgc ccaccagcat gccggagaac aggaactgat agcgcggatc ggcacgcagt
     2221 tcatccagcc tggtcttgat gcgcatgtag ggggcggtgg tggtggcctt gtccagcttg
     2281 cccatctcgt tcgagaggat cgtcgagaga tcggacagga gataggggat cggcgaatcg
     2341 acggtgatgc gcatctgctc ggccagccgg ttgcgcgatc gggccaggtg gaggcatttc
     2401 gccaggatct cggcctcctg ctggcgggca tccccgcgcg aggagagcag gatctcgcaa
     2461 tgctcctcga aattcatcag ccagtagggc agctgcaggt tcgacacatc gaggatcatg
     2521 ccggtatcgc gaaaggccgc gccatattcg ccatgcgggt ccaccatcag cacatggcca
     2581 tggggcgagg catcgcagat gcggtggagg atcagcgcca gcgcggtgga tttgccggtg
     2641 ccggtggagc ccagcagggc aaaatgcttc gacagcagcg gatcgacata gaggctggcg
     2701 gcgatatcgc tggtggggtg cacgcggccg atggcgatag tggcgcggct gccatcgccg
     2761 gcataggcaa tggccagatc ggccgtcgtc accggcacca ccggcgcgcc gggcagcgga
     2821 taatgggtaa cgccgcggcg gaaggcatgg agcgcgccgg tggcaggatc gccctcgccc
     2881 tcgcccatga aatcgatcgc ggcgcggatc tcgccggccg gcccccggcg cagatcgcgc
     2941 aaggtggcca gcagccagcg cccgccagac cacactttca gcgggcagcc cacctgcccc
     3001 gccagcgcca cggccggatc ggcatctccg gcaaaatgcg cttgcgctgc cggatcgaca
     3061 gcgatgaggc tgccgctgcc cgacacttcc agcacctcgc ccagccgcac gcgcgcgcca
     3121 ttgccggccg cagggttcaa aggctccatc accgtcatgc gcgcgatcct tgtcgccggc
     3181 gcgaaggccc ggcccatcat cggccagcag ataggcaggg ctggttaatt ttgcgcaaaa
     3241 atgcgcagct catccccgca cgaacaggcg cccggccacc cagcccatcg ccaccgacac
     3301 cgccaccgcc agcaggccat agagcagccc gtcgtgccgg gcatgatcgg ccacggcctt
     3361 ttcaaagccg atcttcttca cctccacatg ggccagcgcc gaggccacca cccggccctt
     3421 gcggatggcg aaggtctcgg cggtgtaggt gccggtctgc acgctggagg gcagctggat
     3481 gcgcgccttg taaagcacct gcccggtgat ctccaccgca tgctcgtcct gccggtagag
     3541 ccccttgcgg cccatctgct ccaccaggcc ggcggcgaaa tggttctgcc gcgcctcgtc
     3601 caccgcctcg gccggcgaca gctgcagcga gcccagcccc agctcgtaga tcgccgcggt
     3661 gcgttcatcc acaatatcgc gcaagggccg cgaggaggcc accgcataga agctgggcac
     3721 cgacctgagg gtgatactgt cggcattgac ccagatgccg gccatcttgc gcttctcgcg
     3781 cagcaccatc ggccgggccg ggccttccag caccaccacc acgtcatagt cgcgcgcggc
     3841 gcgggccccc tcgggcgtga ggatggcgcc aaacagcagc agttcggtgc cggtgaagcc
     3901 ctggcgcacc tgcacctcgc cctgcgacac atcgggcacc agcagcggct cgcccgcgcc
     3961 catcagcgca aagcccgcaa tggcagccag cgccgcgcgt ttcacagcgg gctcaccgtg
     4021 aagatctcgt cggggtgata ggtgaggccc agcgccatgc gcgcgcccac cgccagcacg
     4081 atcagcgcca ggatcagccg gaggcgcgcc gggcttgcct tcatggcaaa cagcgtgccc
     4141 agctgcgccc ccgaaaccga gccgaacagc agcagcaccg ccagcacgat atccaccgca
     4201 tgggtggtca aggcatgcat catggtggtg gccatggtga cgaagaggat ctggaacagg
     4261 ctggtgccca ccaccacatt ggcccccatg cccagaatat acagcatggc cggcaccagc
     4321 acgaagccgc cgccaatgcc catcagcatc gtcagtatgc cggtgacaag gcccagcagc
     4381 agcggcgcca gcggcgagat atagaggccc gagcgataga agcgccagcg ccacggcagc
     4441 gcggccacca gcgggtggtg ccggcggcgg gccacgggcc gcgtctcgcc gcggcgggtg
     4501 gcggcgaggg tc
//
LOCUS       DASRYE010000237         9865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_166977,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000237 DASRYE010000000
VERSION     DASRYE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166977"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VFF98_12240"
     CDS             complement(<1..1382)
                     /locus_tag="VFF98_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HZU64445.1"
                     /translation="MNSDAMSEILTVESVMQNSGVQFGTSGARGEVVAMTDFVCYAYT
                     CGFLAYLAEIGEFSAGMQVALAGDLRPSTPRILAACAQGVRDSGGEPVFCGFIPTPAL
                     ALFAFGRAIPSLMVTGSHIPDDRNGIKFYRPHGEVLKSDEAGMKRQELAADDALFDGA
                     GMLAKPLPLPAVTDATEAYVARYTGFFGTGALAGKRVGVYQHSAVGRDVLLRILTELG
                     AETVALGRSAQFIPVDTEAIRPEDVVLARGWSAEHGLDAIASTDGDSDRPLLADETGE
                     WLRGDVLGVLCARALGAAAVVTPVSSNTVLERSGAFATTRRTRIGSPFVIDVMNALLA
                     EGQAGVCGYEANGGFLLASPAERGGKILPALPTRDAVLPIVAALVAAGAGSLSAAQAD
                     LPPRVTHSDRIQNFATARSEALFAALLEGDEAARLERLNRHFAAIAGAITAIDLTDGI
                     RMSCTGGAIL"
     gene            1746..4610
                     /locus_tag="VFF98_12245"
     CDS             1746..4610
                     /locus_tag="VFF98_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HZU64446.1"
                     /translation="MTSYLNALGVNLYTSATKVNSFWGAALGQTLTGTSGADGLRGNG
                     IGDVLVGGAGDDTYYIYSTLDQVVEQPNGGTDTVVSGAPSYILPANVENLTMTSTEWY
                     GSGNGLDNIITSQAPYQQMEGGGGNDVFIGSTYTTFIIDAGKGSDRIYNWSSTDTIRL
                     NGYGITSFAQVQSIATQVGSDVVLRFANGDILDLTGVTLGQLTAANFELGLSTSGMKL
                     AFDGEFNSLSLYNAATGTGIWRDEYGYGGAGTISSRTLPGEAEIYMDPSFAGTGTSAL
                     GINPFSVNNGILSITAAPVTAAAAPYLAGHTYTSGLLTTKYSFSMEYGYFQATVKMPS
                     GAGFWPAFWLLPSNGSWPPELDVFEMLGKDPTTVYMTSHYTTATSSNAQTQSLAYVDT
                     TQWHTYGVDWEATTLTFYIDGVAVAQMATPSDMNTPMYMLLNLAVGGTNSWGGGANAS
                     TGTGVMQVSSVKAYATAGTVQETVNGVTTYFTNGNATSTATTLTSGTNGAPPVANADS
                     FATKECAALTVSAANGVLANDTSSTNLALSAVLDTTTLHGKLVLNADGSFSYTPTAGY
                     VGADSFTYQAEDSKGNFSSATTVSLNVTQVTPTANADSFAGKEGTALSVSAANGVLAN
                     DTGASGLALSAVLGTTTQHGALALNADGSFTYTPNAGYSGTDSFTYQAKDSTGALSSA
                     ATVTLNVAQVLPSATSYTGYTTAYEAPLTIAATTLLAGASDAAGYPLSVTGVSNAVGG
                     TVSFSNGLITFTPASWYSGAASFSYTVADAYGDTATASVNLSVGAEVKPSSLYIYGGT
                     ANQTIDKHLSSFGWMINAGSGNDIILGGAGSNSLNAGTGNTTLIGGASADLLQAGTGV
                     DVLTGGGGADTFLFAAGTLHKLGSAGGIDSITDFISGGVASVHDVIKFSGFGAGASLS
                     YTSTNTDGSFNYHVTAGSYSGDLIIQSGGHKLVAADYLFM"
     gene            4741..5754
                     /locus_tag="VFF98_12250"
     CDS             4741..5754
                     /locus_tag="VFF98_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HZU64447.1"
                     /translation="MQLVHYLSTTPNFGDDLNRDIWPALAPGLFADAGDGRGFVGIGT
                     IIGMPDVPDMRLEVFSSGAGYDALAAWRGRDVVYHCVRGPVSARLCGLDPALALTDGA
                     ILAPLVPGLPDAAGGGASGGGGIAVVPHFQTLAGGGWAEACAAAGMRLVDPRQNPHAV
                     ISALAGADLVLTESLHGAILADSYGVPWRAFATSRNFGLAKWIDWTASVGLPMELTAL
                     PPPTAEPWLRFGKRAEPFGLAQSYGLEDAEREFAGRVAPAIASGSSGLKGAAKWLAHS
                     ALRAAPPLQAALGLGVGRTATALADLARQGPMLSASSLRRELTARMAERLAQVERSCL
                     IPA"
     gene            complement(5788..7251)
                     /locus_tag="VFF98_12255"
     CDS             complement(5788..7251)
                     /locus_tag="VFF98_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU64448.1"
                     /translation="MNQMQAIAPAAPADAAPAHAARGPSGRSGVLWSALNGLAGVVVP
                     FVVFAAFARLMTPAQFARFAVALAALELLKALGPMGLYDILVTREESDTTAHRGANSW
                     LLGGAALLALAYAGCLALADPLLGMVMPWPTYLLCGKLLLDYALMQPQAMLVRRKEVR
                     RLGSRSLIAGLAGGVGGFAVGLVATPELGLALFYLLQAAVSFGATVWRTDSWRWPALR
                     ASDARPMMAQGIRASGVRLAGAANSYLDQLVVGRVFAPALVGDYNLGKRLEVVSITIA
                     GSCAQILWQRAFATGDLADRRRALAPGIASAALLCGVPVAGFTVLHAQAVGLIFGRQW
                     QSAALVAALLALSGGMRALGSVAGAMLTVTGRNGRMLALSLFAASSNLAVILALAPFG
                     LEATAYGVLLRNMGQCLLQFAALGEMRRHAMGLLWSHFAVPVGVSALACALAAWAAQG
                     ALLAWAMPDTLRGLLVLAAGGLAGGLVGLRLLWRRMG"
     gene            complement(7248..8303)
                     /locus_tag="VFF98_12260"
     CDS             complement(7248..8303)
                     /locus_tag="VFF98_12260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU64449.1"
                     /translation="MAQAPLLNGPQVSVLMVARNTGPFIGAAIASARAQSVADIEIIV
                     VDDGSEDETRAIAESHAAADARVRVLAGPGAGLAAVRNASLDAARGRRALILDSDDLL
                     APDHVAGLLALAESSGAPMVAANMVSFREGGSEAGAWLFASGQGWQAEREIDLATFLR
                     ANCLFGAGPNLGYLKPLFDMDFLRRHALRYASGLRIGEDFDLLLRAMLAGGQLCFTPR
                     ASYFYRQHAGSTSARLPLADIETLIAAEAAYALPETGDIAAAARARRASLHAAAGHAR
                     AVAALKRRDGAAALAALGGNWHAWQLLGRSCVEAVAKRCGLAAGHRPAPEADAAARHL
                     LQAQDQAARNRAQAVPA"
     gene            complement(8282..9262)
                     /locus_tag="VFF98_12265"
     CDS             complement(8282..9262)
                     /locus_tag="VFF98_12265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU64450.1"
                     /translation="MSVLIVVPCLNEERHLPQLLPALLAENPEALVVVADGGSTDGSR
                     AIVADLARTHPQLVLMDNPRRLQAAGVNLAARRHGGGGRWLVRVDAHCAYPAGYVAGL
                     VAAAEQVGATSVVVPMVTAGITCFQSAVAAAQNSRLGNGGSPHRNLGQGRWVDHGHHA
                     LFDMALFLAVGGYDESFSHNEDAELDVRLAQAGGRLWLEPSLALTYFPRRAPAPLLAQ
                     YRNYGRGRARNRRRHRLRLALRQIVPLAVAPLVCLALLGLLAAGVDRRALWLAVPALA
                     WAGLCLAYGGALAWRQRSSCVLCAGLAAMLMHLGWSFGFLEEQLWPRHPS"
     gene            complement(9259..>9865)
                     /locus_tag="VFF98_12270"
     CDS             complement(9259..>9865)
                     /locus_tag="VFF98_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64451.1"
                     /translation="PPAPAGGRPWVIGWFGMLRCQRTLEMLGRLAAGSGGRIEVVIAG
                     IPSAAVFPDFAAQVAAMPGLRYLGPYTKADLPRLYGQIHFVWAIDYFEEGLNSAWLLP
                     NRLYEGLAHGAVPLALGDVATGSWLHRHGAGLLLGAPAADLPARLQALDAAGLARLRA
                     EVDAIPDEAIWQTAAERAAIASAICGAAAAATTPARLEVAA"
BASE COUNT         1758 a         3562 c         3109 g         1436 t
ORIGIN      
        1 tgcaggatcg ccccgccagt acagctcatg cggatgccat cggtcaggtc gatcgcggtg
       61 atggcgccgg cgatggcagc aaaatgccgg ttcagccgct ccagccgcgc cgcttcatcg
      121 ccttccagca gggcggcgaa cagggcctcg ctgcgggcgg tggcaaagtt ctggatgcgg
      181 tcggaatggg tgacgcgtgg cggcagatcg gcctgcgcgg cgctgaggct gcccgcgccc
      241 gccgccacca gcgcggcaac gatcggcagc accgcatcgc gcgtgggcag cgccggcagg
      301 atcttgccgc cgcgctctgc agggctggcc agcaggaaac cgccattggc ttcatagccg
      361 cagacgcccg cctgcccttc ggccagcagg gcgttcatca catcgatcac gaagggcgag
      421 ccgatgcgcg tgcgccgcgt ggtggcaaag gcgcccgaac gttccagcac cgtgttgctg
      481 gacaccggcg tcaccaccgc cgctgccccc agcgcgcggg cgcacagcac gcccagcaca
      541 tcgccgcgca gccattcgcc ggtttcgtcg gccagcagcg gccggtccga atcgccatcg
      601 gtggaggcaa tcgcatccag cccgtgttcg gccgaccagc cgcgtgccag caccacatcc
      661 tcggggcgaa tggcctcggt atcgaccggg atgaactgcg cagagcggcc cagcgccacc
      721 gtttcggcgc ccagttccgt caggatgcgc agcagcacat cgcgccccac ggcggaatgc
      781 tgatacaccc ccactcgctt gccggccagc gccccggttc cgaaaaaccc cgtataacgg
      841 gccacatagg cctcggtggc atcggtgacg gcaggtaggg gcaagggctt agccagcatg
      901 cctgcgccat cgaacagggc atcatcggcg gccaattcct gccgcttcat gccggcctca
      961 tcgcttttca gcacttcgcc atgggggcgg tagaacttga tgccgttgcg atcgtcgggg
     1021 atgtggctgc cggtgaccat tagcgagggg atcgcccggc caaaggcaaa cagcgccagc
     1081 gccggcgtgg ggatgaagcc gcagaacacc ggctcgccgc ccgagtcgcg cacgccttgc
     1141 gcgcaggccg ccaggatgcg cggcgtggag gggcgcagat cgcccgccag cgccacttgc
     1201 atgcccgcag agaactctcc gatttcagca agataggcaa ggaagccgca ggtgtaggcg
     1261 tagcagacga aatcggtcat ggcgacaacc tcgccccgcg cgccgctggt gccgaactgc
     1321 acgcccgagt tttgcatcac gctttccacg gtgagaatct cgctcatcgc atcgctgttc
     1381 atcgcgcatt tcctgtgttg tcggcgcccg gccattccag accacgtgat tctccgcagc
     1441 ctgcttttgt ttcttaagca gcacaatgga ttgggcagca agagcctgtg tgaacggcct
     1501 gccgctttcc aggtgttgtc aggcaacaag atatcgtctg tgggaagtct ctcaattgac
     1561 atgcgcgcaa tgtattttac gaagatgttc ggttaaataa aattcacatc atctatgcaa
     1621 aatcacataa tcctgtgtaa atcatttata aatcatgact tacggtggtt aactttaaaa
     1681 tgtggtaata attttagggc ggcaatgaga aatcgtcttc gtaatccagt tcgaggatga
     1741 agaagatgac gtcttacctg aatgccctgg gcgtgaacct ctacacttcg gccacgaagg
     1801 tgaattcctt ctggggcgct gcgcttggcc agactttgac cggcacctcg ggggcagatg
     1861 gcctgcgggg taatggcatt ggcgatgtgc tggtgggggg cgccggcgac gacacctact
     1921 acatctactc caccctcgat caggtggtgg agcagccaaa cgggggaacg gatacggtgg
     1981 tttcgggcgc gccatcctat atcttgccgg ccaatgtcga gaacctcacc atgacctcga
     2041 cggaatggta tggttcgggc aacgggctcg acaatatcat cacctcgcag gccccctatc
     2101 agcagatgga aggcggcggc ggcaatgatg tcttcatcgg ctccacctac accaccttca
     2161 tcatcgatgc cggcaagggc agcgatcgca tctacaactg gtcgtccacc gacacgatcc
     2221 gcctcaatgg ctatggcatc actagcttcg cacaggtgca aagcatcgcc acccaggttg
     2281 gcagcgatgt ggtgctgcgc tttgccaatg gcgatatcct cgacctgacc ggcgttacgc
     2341 tgggccagtt gaccgccgcc aatttcgagc tgggcctcag caccagcggc atgaagctag
     2401 ccttcgatgg cgagttcaac agcctcagcc tgtataacgc ggccactggc accggcatct
     2461 ggcgcgacga atatggctat ggcggcgccg gcacgatctc cagccgcacc ctgcccggcg
     2521 aggccgaaat ctacatggat cccagctttg ccggcaccgg cacctcggcg ctgggcatca
     2581 accccttctc ggtgaacaac ggcatcctgt cgatcaccgc agcgcccgtc accgcggcag
     2641 cggcgcccta tctggccggc cacacctata cctcgggcct gctcaccacc aaatattcct
     2701 tctcgatgga atatggctat ttccaggcga cggtgaagat gccctcgggc gcgggcttct
     2761 ggccggcctt ctggctgctg cccagcaacg gcagctggcc gcccgaactc gacgtgttcg
     2821 agatgctggg caaggacccc accaccgtct acatgaccag ccactacacc accgccacca
     2881 gcagcaacgc ccagacgcaa agcctggcct atgtcgatac cacccagtgg cacacctatg
     2941 gcgtggattg ggaagcaacg acgctgacct tctatatcga tggcgtggcc gtggcgcaga
     3001 tggccacccc atcagacatg aacacgccga tgtacatgct gctgaacctg gccgtgggcg
     3061 gcaccaacag ctggggcggc ggcgccaatg ccagcaccgg cactggcgtg atgcaggtgt
     3121 cgagcgtgaa ggcctatgcc acggccggca cggtgcagga aacggtgaac ggggtgacca
     3181 cctatttcac caacggcaat gccaccagca ccgccaccac cctcaccagc ggcaccaatg
     3241 gcgcgccgcc cgtggccaat gccgacagct tcgccacgaa ggaatgcgca gccctcaccg
     3301 tgtcggccgc caatggcgtg ctggccaacg acacctcttc caccaacctg gccctttccg
     3361 cggtgctgga taccaccacc ctgcatggca agctggtgct caacgccgat ggctccttca
     3421 gctatacgcc gacggccggc tatgtcggcg ccgacagctt cacctatcag gccgaggatt
     3481 cgaagggcaa tttcagctct gccacaacgg tgagcctgaa cgtgacccag gtgacgccca
     3541 ccgccaatgc cgacagtttc gccggcaagg aaggcacagc gctcagcgtg tcggccgcca
     3601 atggcgtgct ggccaacgac accggcgcca gcggccttgc gctttcggcg gtgctgggca
     3661 ccaccaccca gcatggcgcg ctggcgctga atgccgatgg ctccttcacc tatacgccca
     3721 acgccggcta ttcgggcacc gacagcttca cctatcaggc caaggatagc acaggcgcgc
     3781 tcagcagcgc ggccaccgtc acgctgaatg tagcgcaggt gctgcccagc gccaccagct
     3841 acaccggcta taccaccgcc tatgaggccc cgctgacgat cgctgccacc actctgctgg
     3901 ccggcgccag cgatgccgcc ggctatccgc tgagcgtgac gggcgtgagc aatgcggtgg
     3961 gcggcacggt gtccttcagc aacgggctga tcactttcac gccggccagc tggtatagcg
     4021 gggcggcctc cttcagctac accgttgccg atgcctatgg cgacaccgcc accgccagcg
     4081 tcaacctctc tgtgggcgcc gaagtgaagc cgtcctcgct ctacatctat ggcggcaccg
     4141 ccaatcagac gatcgacaag cacctgtcga gctttggctg gatgatcaac gcgggctcgg
     4201 gcaacgacat catcctgggc ggcgccggca gcaattcgct gaacgcgggc accggcaaca
     4261 ccacgctgat cggcggcgcc agcgccgacc tgctgcaggc cggcaccggc gtggacgtgc
     4321 tgaccggcgg cggcggggcc gacaccttcc tgtttgccgc cggcacgctg cacaagctgg
     4381 gcagcgccgg cgggatcgac agcatcaccg atttcatcag cggcggcgtg gccagcgtgc
     4441 atgacgtgat caaattctcc ggctttggcg ccggcgccag cctcagctac acctccacca
     4501 acaccgatgg ttccttcaac tatcacgtca cggccggcag ctattcgggc gatctcatca
     4561 tccagagcgg cgggcacaag ctggtcgcgg cggattacct gttcatgtaa tggggagggc
     4621 cggggcagac ggggccccgg cccgtttccg gatcaatccg aaaccgatgc gagcatgggt
     4681 tccggcaaac ccccgcttca cgatacgact gacggcaaag aggcgcaaag aggggtcgcc
     4741 atgcagcttg tccattatct cagcaccacg cccaatttcg gcgacgatct caaccgcgac
     4801 atctggcccg ccctggcgcc cggcctgttc gccgatgctg gcgacgggcg gggctttgtc
     4861 ggcatcggca cgatcatcgg catgcccgat gtgccggata tgcggctgga ggtgttctct
     4921 tccggcgccg gctatgatgc gctggcggcc tggcgggggc gcgatgtggt ctatcattgc
     4981 gtgcgcgggc cggtcagcgc gcggctgtgc gggctcgatc cggcgctggc gctgacagat
     5041 ggcgccattc tcgcgccgct ggtgcccggc ctgccggatg cggcgggcgg cggggccagc
     5101 ggcggcggcg gcatcgccgt ggtgccccat ttccagaccc tggccggcgg cggctgggca
     5161 gaggcgtgcg cggccgccgg catgcggctg gtcgatccgc ggcagaaccc gcatgcggtg
     5221 atctcggcgc tggccggggc cgatctggtg ctcaccgaat cccttcacgg ggcgatcctg
     5281 gccgacagtt atggcgtgcc ctggcgcgcc tttgccacct cgcgcaattt cggcctcgcc
     5341 aaatggatcg actggaccgc ctctgtcggc ctgccgatgg agctgaccgc cctgcccccg
     5401 cccacggccg aaccctggct gcgctttggc aagcgggcgg aacccttcgg tctggcccag
     5461 agctatgggc tggaagatgc ggagagggaa tttgccggcc gggtggcgcc ggccatcgcc
     5521 agcggcagct ccgggctgaa aggcgcggcc aaatggctgg cccattccgc cctgcgcgcg
     5581 gcgcccccct tgcaggcggc gctggggctg ggcgtgggcc gcacggccac cgcgctggcc
     5641 gatctggcgc ggcagggccc gatgctcagt gcctccagcc tgcgccgcga attgaccgcc
     5701 agaatggccg agcgcctggc gcaggtggag cgcagctgcc tgatcccggc ctgatgcggc
     5761 ggcggcggcc tgctgccggg gccgccgtca gcccatgcgg cgccatagca ggcgcaggcc
     5821 caccaggccg ccggccagcc cgcccgccgc cagcaccagc aggccgcgca acgtgtccgg
     5881 catggcccat gccagcagcg cgccctgcgc tgcccatgcc gccagcgcgc aagccagcgc
     5941 actcaccccc actggcacgg cgaaatggct ccacaacaga cccatcgcat gccggcgcat
     6001 ttcacccagc gcggcaaact gcagcaggca ctggcccatg ttgcgcaaca aaacgccata
     6061 ggccgtggcc tccagcccga acggggccaa agcgaggatc accgccagat tgctgctcgc
     6121 ggcaaacagg ctgagcgcca gcatccggcc attgcgcccg gtaaccgtca acatcgcgcc
     6181 ggccacactg cccagcgccc gcatcccgcc agacagggcc agcagcgccg ccaccagcgc
     6241 cgcagattgc cactgccggc cgaagatcag ccccaccgcc tgggcatgca gcaccgtaaa
     6301 gccggccacc ggcaccccgc acagcagcgc cgcgctggcg atgccgggcg ccagcgcgcg
     6361 ccgccggtcg gccagatcgc cggtggcgaa ggcgcgttgc cacaggatct gcgcgcagga
     6421 accggcgatc gtgatcgaca ccacttccag ccgcttgccc agattatagt cgcccaccag
     6481 cgccggcgcg aacacccggc ccaccaccag ctgatcgaga tagctgttgg ccgcgcccgc
     6541 caaccgcacg ccagaggccc tgatgccctg cgccatcatc ggccgcgcat cgctggcacg
     6601 cagggcgggc cagcgccagc tatccgtgcg ccacaccgtg gccccgaaag acaccgccgc
     6661 ctgcagcaga tagaacagcg ccagccccag ttccggcgtg gccaccagcc ccacggcaaa
     6721 gccgcccacg ccgcccgcca gcccggcaat caggctgcgc gagccaagcc tgcgcacttc
     6781 cttgcggcgc accagcatgg cctgcggctg catcagcgca taatcgagca gcagcttgcc
     6841 gcacagcaga taggtgggcc agggcatcac catgcccagc agcggatcgg ccagcgccag
     6901 gcagcccgca taggccagcg ccagcaaggc cgcgccgccc agcagccagc tgttggcgcc
     6961 gcgatgggca gtggtatcgc tctcctcccg cgtgacgaga atgtcgtaaa gccccatcgg
     7021 gcccagcgcc ttcaacagtt ccagcgcggc cagtgccacg gcgaaacggg cgaattgggc
     7081 cggtgtcatc aaccgggcga aagcggcaaa gacgacaaac ggcacgacca ccccggccag
     7141 cccgttcaac gccgaccaca agacgcccga tcgcccgctt ggcccgcgcg ccgcatgagc
     7201 gggcgcggca tcggcggggg cggcgggggc gatcgcctgc atctggttca tgccggcacc
     7261 gcctgcgccc ggttgcgggc agcctgatcc tgcgcctgca acagatggcg cgcggccgcg
     7321 tctgcctctg gcgcggggcg atggccggcg gccaggccgc aacgcttggc cacagcttcc
     7381 acacagctgc gccccagcaa ctgccaggcg tgccagttcc cccccagcgc cgccagcgct
     7441 gccgccccat cccggcgctt cagcgcggcc acggcccggg catggccggc cgccgcatgc
     7501 aaactggccc ggcgcgcccg cgcggcagcg gcgatatctc ccgtctcggg caaggcatag
     7561 gcagcttcgg cagcgatcag cgtctcgata tccgccagcg gcaggcgcgc ggaggtggag
     7621 ccggcatgct ggcgatagaa atagctggcc cggggggtga agcacagctg cccgccggcc
     7681 agcattgccc gcagcagcag gtcgaaatcc tccccgatgc gcaggccgct ggcatagcgc
     7741 agcgcgtgcc ggcgcaggaa atccatatcg aacagcggct tcagatagcc cagattgggc
     7801 ccggcgccga acaggcaatt ggcacgcagg aaggtggcca gatcaatctc acgctctgcc
     7861 tgccagccct ggccgctggc gaaaagccac gccccggcct cgctcccgcc ttcgcgaaag
     7921 ctgaccatat tggcggccac catcggcgcc ccgctgcttt ctgccagagc cagcaggccc
     7981 gccacatgat cgggcgccag cagatcgtca ctgtcaagga tcagcgcccg gcgccccctt
     8041 gccgcatcaa ggctggcatt gcgcaccgcg gccagccccg cccccgggcc tgccagcacg
     8101 cgcacccgcg catcggccgc ggcatggctt tcggcaatgg cgcgcgtctc atcctcagag
     8161 ccatcgtcca ccacgataat ctcgatatcg gccacgcttt gcgcccgtgc ggaagcgatc
     8221 gccgcgccaa tgaagggccc cgtgttgcgc gccaccatca acaccgacac ctgcggcccg
     8281 ttcaagaggg gtgcctgggc cacaattgct cctccagaaa gccgaaagac cagcccagat
     8341 gcatcagcat cgccgccagc ccggcacaca acacgcaaga tgaacgctgc cgccacgcca
     8401 gcgcgccgcc ataagccagg cacaggcccg cccaggccag cgccggcacg gccagccaca
     8461 gcgcccgcct gtccaccccg gcggccagca ggcccagcag cgccaggcac accagcggcg
     8521 ccaccgccag cggcacgatc tgccgcagcg ccaggcgcag ccggtggcgc cggcgattgc
     8581 gcgcccggcc ccggccataa ttgcgatatt gcgccagcag cggcgcgggc gcccggcggg
     8641 ggaaataggt caacgccaga gacggttcca gccagaggcg cccgcccgcc tgggccaggc
     8701 gcacatccag ctcggcatcc tcattatgtg aaaaactttc gtcatacccg cccacggcga
     8761 ggaacagcgc catgtcgaac agcgcatggt ggccatgatc cacccagcgg ccctgcccca
     8821 ggttgcgatg cggcgatccg ccattgccca gccgcgaatt ctgcgccgcc gccacggctg
     8881 actggaagca ggtgatcccc gccgtcacca tcggcacgac gaccgaggtt gcccccacct
     8941 gttccgccgc ggccaccaac ccggccacat agcccgccgg ataagcgcaa tgggcatcca
     9001 cccgcaccag ccagcggccg ccgccgccat ggcgccgcgc cgccagattg acgcccgccg
     9061 cttgcagccg gcgcggattg tccatcaaca ccagctgcgg atgggtgcgc gccagatcgg
     9121 cgacgatggc gcggctgcca tcggtgctgc cgccatcggc caccaccacc agcgcctccg
     9181 ggttttccgc cagcagggcg ggcagcagct ggggcagatg ccgctcctcg ttcaggcagg
     9241 gcacaacgat cagcacgctc atgccgccac ctccagccgg gcgggtgtgg tggcagcggc
     9301 ggccgcaccg caaatggcgc tggcgatcgc cgcgcgttct gccgccgtct gccagatggc
     9361 ctcgtcgggt atggcgtcca cctcggcgcg caggcgggcc agccctgcgg catccagcgc
     9421 ctgcaggcga gcgggcaggt ccgctgccgg cgcccccagc agcaggccgg cgccatggcg
     9481 atgcagccag ctgccggtgg ccacatcgcc cagcgccagc ggcaccgcgc catgggccag
     9541 cccttcatac aggcggttgg gcagcagcca ggcggaattc agcccttcct cgaaatagtc
     9601 gatcgcccag acgaaatgga tctgcccata aaggcgcggc agatccgcct tcgtgtaggg
     9661 ccccagatag cgcagccccg gcatggcggc cacttgcgcg gcaaaatcgg gaaagaccgc
     9721 ggctgaggga atgccggcaa tcaccacctc gatccgcccg ccgctgccag cggccaggcg
     9781 ccccagcatc tccagcgtgc gctggcagcg cagcatgccg aaccagccga tcacccaggg
     9841 cctgcccccg gcgggcgccg gcggg
//
LOCUS       DASRYE010000238         7439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_14110,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000238 DASRYE010000000
VERSION     DASRYE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14110"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFF98_12275"
     CDS             <1..560
                     /locus_tag="VFF98_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU64452.1"
                     /translation="ETGQQRRAPAPAGPLAEEARAKARAVAYRAGVKHISVPELELLQ
                     ADPARTLYRYDVRLPEEYAQGHLPGFRNAQGGQLVQETDHFAPVRGARIVLFDPLGAR
                     ADMTASWLAQLGWETYVLAADPQPAAWTGATEEAPPPRGPEGRYRRPYEGTDQAHAAM
                     QAYLDWEYGLVAQLERDGTHGFFVI"
     gene            754..1542
                     /locus_tag="VFF98_12280"
     CDS             754..1542
                     /locus_tag="VFF98_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZU64453.1"
                     /translation="MAVIAVYSPKGGVGKTTISVDLAWRCAAIGGLRTLLWDLDPQGG
                     SGFLLGAEQGRRQAAASVFQREGRPERLAMPTAYPGLSLLGADDSLRSLPLHLARLGQ
                     RRRLATLTRTLGGAFERIVLDCPPMQNEVSEQILHAADVVIVPLPVSPLAARGLDLLR
                     ADMARHHGSHPPILPVLSMYDPRRQSHRAAREGHMAHFPVVPMASQIEQVAFQRAPIG
                     TFAPHCPPARALDRLWRAIEAKLAATGTAGALPAAAPPPVAARA"
     gene            complement(1609..2088)
                     /locus_tag="VFF98_12285"
     CDS             complement(1609..2088)
                     /locus_tag="VFF98_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZU64454.1"
                     /translation="MTIAVGQSLPDVKLVKSSEDAREPVQTAEYFKGKKVALFSVPGA
                     FTPTCSAKHLPGFVEKAADLKAKGIDEIVCTAVNDPFVMGIWGAQKGSTDITMLADGN
                     GDFAEALGLTMDGSGFGLGKRGQRFALVVNDGVVEQVFVEAPGEFKVSSAEHVLESL"
     gene            2316..2777
                     /locus_tag="VFF98_12290"
     CDS             2316..2777
                     /locus_tag="VFF98_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-L-homocysteine hydrolase"
                     /protein_id="HZU64455.1"
                     /translation="MTGWTKWAGALPAAALLLAPVGAGAAGSASNAEKLRRLDIMLMV
                     TALRCRSGTDDFQTDFQAFEGHHLAELNQAAHQLRDELAAREGAVAADRALDRLSVVV
                     ANQYGGGHPWLGCHELKGLAHELAREDGVAPLLDAADATLKGDAPVLAALP"
     gene            2936..4351
                     /gene="ahcY"
                     /locus_tag="VFF98_12295"
     CDS             2936..4351
                     /gene="ahcY"
                     /locus_tag="VFF98_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZU64456.1"
                     /translation="MIPVSTADLTKDYVIADIGLADYGRAEIAIAETEMPGLMALRAE
                     FGETKPLKGARITGSLHMTIQTAVLIETLVALGAEVRWATCNIFSTQDHAAAAIAAQG
                     IPVFAIKGESLADYWDYVGRIFDWGDNHTANLILDDGGDATMFALWGARVEAGEDLFE
                     PSNAEEIEFCRALKAFLKAKPGYLSRSVNAIRGVSEETTTGVHRLYHIAKDGKLPFPA
                     INVNDSVTKSKFDNLYGCKESLVDAIRRATDVMLAGKVACVAGFGDVGKGSAASLRNG
                     GARVLVTEIDPICALQAAMEGYEVVTMEEATKRADIFVTCTGNEDVITAEHMAAMKPM
                     AIVCNIGHFDSEIQISALSNYHWTEVKPGTDLVTFPDGKQIIVLAKGRLVNLGCATGH
                     PSFVMSASFTNQVLAQIELYTKAAEYENRVYVLPKHLDEKVAALHLAKLGVQLTRLSD
                     KQAKYIGVPQAGPFKADHYRY"
     gene            4522..6900
                     /locus_tag="VFF98_12300"
     CDS             4522..6900
                     /locus_tag="VFF98_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HZU64457.1"
                     /translation="MTMNHAALVLLGLALAGWMLVAGRLWLAGRQALARAQGMQGMLA
                     RLARMVEENPAQLLVVHADGRLEGSARLAGWLGLEQLPPLLERLDNTPGQGLAPAQLA
                     SLREAMGASQRSSRPFRLTLTPPGGQRTLVLRGSQADPQVAPAGAMQIWVFDNSESAQ
                     ALARLQVDAARARGDFSALVGLIEAAPMPMWFRGQDGALRLVNSAYVAAVGGESAAQV
                     VTDGVELVEPIDGQTPAQVALGAAGRGEAVERLVSATIDGQRRAMRVNDLPLGAEGIA
                     GYAVDVEDMEELARSLRAFREAQRSMLDLLSAGVAQFDAGRRLNFANQPFQRLLALKP
                     ADVPVGQGFERLLDLAREGGRVPELRDYPAWRRERARWFAHNGAQEEAWPLSDGTHLR
                     VVAQPMPDGGLLLIVEDRTETLRIAAMRDTLLRTRTATFDSLFEAVAVFAPDGRMQLW
                     NRRFASAWGLESELLDSHPHIGDLLKAVAPGLAQAEEARALGDVVRAATLDRRQTEAR
                     LKLADGRVLEFAGVPLPDGNGLLTVLDITDAQKAEAALRERNAALAEADRLKTRFLAN
                     MSYEFRTPLTSITGFAELLQAGLGGELSEGGREYVAAILASSARLAEQIENVLDLSQS
                     EAGLLPMAAEAIDLFPFITAIAGDRSARLGEKHLSLDLRGDRSAGQVRGDPRRLGKAV
                     GHLIDNAIAASPEGARIRLDLVTLRQGPGPGGGRIRIIIADQGPGMDSAMLARALGGL
                     KPDDEPGEGALERRQGLGLPLASQLIEAHGGTLDIHSRPGAGTQAIIELPRL"
     gene            6897..7367
                     /gene="tsaE"
                     /locus_tag="VFF98_12305"
     CDS             6897..7367
                     /gene="tsaE"
                     /locus_tag="VFF98_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZU64458.1"
                     /translation="MTARPFLALPDEAATQRLGARIAAGLQAGDVVALSGDLGAGKTT
                     LARAILRALGHAGEVPSPSFAIIEPYEPPAVRLPVVHADFYRLAHPQEAEELGLEDYR
                     ADGALIAEWPEQAGGFTHEGACLAIHIEVGPEGRRAIVRPGPDWLGRWDDSESA"
BASE COUNT         1124 a         2414 c         2712 g         1189 t
ORIGIN      
        1 tggaaaccgg ccagcagcgc cgcgcgcccg cgcccgccgg cccgctggcc gaagaggccc
       61 gggcaaaggc gcgcgcggtg gcctatcgcg cgggggtgaa gcacatcagc gtgccggaac
      121 tggagctgct gcaggcagac cccgcccgca cgctctaccg ctatgatgtg cgcctgcccg
      181 aggaatatgc gcaaggccat ctgcccggct ttcgcaatgc ccagggcggg cagctcgtgc
      241 aggaaaccga tcatttcgcg ccggtgcgcg gcgcccgcat cgtgctgttc gacccgctgg
      301 gcgcgcgggc cgacatgacc gccagctggc tggcccagct gggctgggaa acctatgtgc
      361 tggccgccga tccgcagccc gccgcatgga caggcgctac cgaggaagcc ccgccgcccc
      421 gcgggcccga gggccgctat cggcgcccct atgaggggac cgatcaggct catgccgcca
      481 tgcaggccta tctcgattgg gaatatggcc tggttgccca gctggaacgc gatggcaccc
      541 acggcttttt cgtcatctga acgccggtga accgcgaatc gccctgccgt cttgatcggg
      601 aaaccgcgcc cgatttcgat cgaattttac ataagcagct ttatttaaaa cacaaatgct
      661 tattctgccg gacgcttgcg gcgcctggca aaaaaaggca tctggcggca gggataacgc
      721 gcgttaagga ttgcggggcg gcatgagcgg gcgatggcgg taatcgcggt ctacagcccg
      781 aagggcgggg tcggcaagac gaccatctcg gtcgatctgg cctggcgctg tgcggccatt
      841 ggcggcctgc gcacgctgtt gtgggatctt gatccgcagg gcggttccgg ctttctcctg
      901 ggggcagagc agggccggcg gcaggcggcc gcctcggtgt tccagcgcga ggggcggccc
      961 gagcggctgg cgatgcccac ggcctatccc ggcctttccc tgctgggcgc ggacgacagt
     1021 ttgcgcagcc tgccgctgca tctggcgcgg ctgggccagc gccggcgcct ggccacgctg
     1081 acgcgcacgc tgggcggcgc ttttgagcgg atcgtgctgg attgcccgcc gatgcagaat
     1141 gaggtgagcg agcagatcct gcatgccgcc gatgtggtga tcgtgccgct gccggtctcg
     1201 ccgctggctg cgcgcgggct ggatctgctg cgggcagaca tggcccgcca ccatggcagc
     1261 catccgccga tcctgccggt gctctcgatg tatgatccgc gccgccagtc gcaccgtgcc
     1321 gcgcgtgagg ggcacatggc ccatttcccg gtggtgccga tggccagcca gatcgagcag
     1381 gtggcctttc agcgcgcgcc gatcggcact tttgcgccgc attgcccgcc ggcccgcgcg
     1441 ctggacaggc tgtggcgggc gattgaggcc aagctggcag ccactggcac ggccggggca
     1501 ttgcccgccg cagcgccgcc gccggtggcc gcccgggcct gaccgcccca aaagcgcgca
     1561 ggccggcggc aggccgcagc ccgccaccgg cccgcaaacc tcaaggcctc agaggctttc
     1621 gagcacgtgc tcggcgctcg acaccttgaa ctcgcccggc gcttcgacga agacctgctc
     1681 caccacgcca tcattcacca ccagcgcgaa acgctggccg cgcttgccca ggccaaagcc
     1741 cgaaccgtcc atggtcaggc ccagcgcctc ggcgaaatcg ccattgccat cggccagcat
     1801 ggtgatgtcg gtgctgccct tctgcgcgcc ccagatgccc atcacgaagg gatcgttgac
     1861 ggcggtgcag acgatctcgt cgatgccctt ggccttgaga tcggcggcct tttcgacaaa
     1921 gcccggcagg tgcttggccg agcaggtggg ggtgaaggcg cccggcaccg agaacagcgc
     1981 caccttcttg cccttgaaat attcagcggt ctgcaccggt tcgcgggcgt cttcgctcga
     2041 ctttaccagc ttcacatccg gcaggctctg gccaacggcg atggtcatgt ctttctcctc
     2101 agggatcttt ggcgaccagc agcgcatcgg gcgcgcctgt cgcctcgcca catatagggc
     2161 gcgggggcgc gggggcaagg tggcggggca ctgtaacgtg gcaggtaaca cggcttaatt
     2221 tcgcttaacc gcaaaggttc aaccggcggc aagccggatc ggcgaacatg gccggcccgg
     2281 caggcgatgg atcgccgccg ggccgggagg agcatatgac gggatggacg aaatgggccg
     2341 gcgccttgcc ggcagccgcg ctgctgctgg cgccggtggg ggcaggggcg gcaggttctg
     2401 ccagcaatgc cgagaaattg cgccggctgg atatcatgct gatggtgacg gcgctgcgct
     2461 gccgcagcgg caccgatgat ttccagaccg atttccaggc cttcgagggc catcatctgg
     2521 ccgagctgaa ccaggccgcg catcagctga gggacgagct ggccgcgcgc gagggggcgg
     2581 tcgcggccga tcgcgcgctc gacaggctca gcgtggtggt ggccaaccag tatggcggcg
     2641 gccacccctg gctgggctgc cacgagctga aggggctggc ccatgagctg gcccgcgagg
     2701 atggcgtggc gccgctgctg gatgcggccg atgccacgct gaagggcgat gcgccggtgc
     2761 tggccgcttt gccctgattg cgcggcgttg ccctgacagg atgcctccgc agtgggatat
     2821 aaagacttct ttatatttgc atgcgggcgg caaggcggct aaaggcggca gggtatggcg
     2881 cgcatggtgg caggctcttc ggggtggccg ccctgtgcgc cgcttgccca ggagtatgat
     2941 ccccgtgtcc accgccgacc ttaccaagga ttatgtgatc gccgacattg gcctggccga
     3001 ttatggccgc gccgagatcg ccatcgccga aaccgagatg ccgggcctga tggccctgcg
     3061 cgccgaattc ggcgagacga agccgctgaa gggcgcgcgc atcaccggca gcctgcacat
     3121 gaccatccag accgccgtgc tgatcgagac gctggtggcg ctgggcgccg aagtgcgctg
     3181 ggccacctgc aacatcttct ccacgcagga tcatgccgcc gccgccatcg ccgcgcaggg
     3241 catccccgtc ttcgccatca agggcgagag cctggccgac tactgggatt atgtcggccg
     3301 catcttcgat tggggggata accacaccgc caacctcatc ctcgatgatg gcggcgatgc
     3361 caccatgttt gccctgtggg gcgcgcgggt ggaagcgggc gaggacctgt tcgagccctc
     3421 gaacgccgag gaaatcgagt tctgccgcgc gctcaaggcc ttcctgaagg ccaagccggg
     3481 ctatctgtcg cgctcggtca acgccattcg cggcgtgtcg gaagagacga cgacgggcgt
     3541 gcaccgcctt tatcacatcg ccaaggatgg caagctgcct ttcccggcga tcaatgtgaa
     3601 cgattcggtc accaagtcga agttcgacaa cctctatggc tgcaaggaat cgctggtcga
     3661 tgccatccgc cgcgcgaccg acgtgatgct ggccggcaag gtggcttgcg ttgccggttt
     3721 cggcgatgtc ggcaagggct cggccgcttc gctgcgcaat ggcggcgcgc gcgtgctggt
     3781 caccgagatc gatccgatct gcgcgctgca ggcggcgatg gaaggctatg aagtggtgac
     3841 gatggaagag gcgaccaagc gcgccgacat cttcgtgacc tgcaccggca atgaggacgt
     3901 gatcaccgcc gagcacatgg cggcgatgaa gccgatggcg atcgtgtgca acatcggcca
     3961 cttcgacagc gagatccaga tctcggccct gtcgaactat cactggaccg aagtgaagcc
     4021 gggcaccgat cttgtcacct tccccgatgg caagcagatc atcgtgctgg ccaagggccg
     4081 gctggtgaac ctgggctgcg ccactggcca ccccagcttc gtgatgtcgg ccagcttcac
     4141 caaccaggtg ctggcgcaga tcgagctgta caccaaggcg gccgaatatg agaaccgcgt
     4201 ctatgtgctg cccaagcatc tggacgagaa agtggccgcg ctgcacctgg ccaagctggg
     4261 cgtgcagctg acccggcttt cggacaagca ggccaagtat atcggcgtgc cgcaggccgg
     4321 cccgttcaag gcggaccatt accgctactg atcggccggt ttccggcagc atggcggggg
     4381 gcgggcgatg gcctgccccc cgtttgcgtt tgcggggggg cgacaggccg ccccccgttt
     4441 gcgctggggc gcaggcggaa tgcggtgaca ggtggcgccc ggggtgttgt caatccgccc
     4501 gcgcttgcct agccaaggat gatgacgatg aaccatgccg cgctggtgtt gctggggctg
     4561 gcgctggccg gctggatgct ggtggcaggc cggctgtggc tggccgggcg ccaggcgctg
     4621 gcccgggcgc aagggatgca gggcatgctg gcccggctgg cccgcatggt ggaggaaaac
     4681 cccgcgcaat tgctggtggt gcatgccgat ggccggctgg aaggctctgc ccggctggct
     4741 ggctggctgg ggctggagca gttgcccccc ctgctggagc ggctggataa cacgccgggc
     4801 caggggctgg cgccggccca gctggccagc ctgcgcgagg cgatgggcgc cagccagcgc
     4861 tcctcgcgcc ccttccgcct gacgctgacg ccgccgggcg gccagcgcac gctggtgctg
     4921 cgcggcagcc aggccgatcc gcaggtggcg ccggccggcg cgatgcagat ctgggtgttc
     4981 gacaatagcg agagcgccca ggcgctggcc cggctgcaag tggatgccgc ccgcgcgcgc
     5041 ggcgatttct cggcgctggt ggggctgatc gaggccgcgc ccatgccgat gtggtttcgc
     5101 gggcaggatg gcgcgctgcg gctggtgaat tcggcctatg tggcggcggt gggcggcgag
     5161 agcgcggcgc aggtggtgac agatggcgtg gagctggtgg agccgatcga cgggcagacg
     5221 ccggcgcagg tggcgctggg cgcggccggg cgaggcgagg cggtggagcg gctggtgagc
     5281 gccaccatcg atggccagcg ccgcgccatg cgggtgaacg acctgccgct gggggcagag
     5341 ggcatcgccg gctatgcggt ggatgtggag gatatggagg agctggcccg ctcgctgcgc
     5401 gccttccgcg aggcgcaaag gtcgatgctc gatctgctgt cggcgggggt ggcgcagttc
     5461 gatgccgggc gccggctgaa tttcgccaac cagcctttcc agcggctgct ggcgctgaag
     5521 ccggccgatg tgccggtggg gcaggggttt gaacggctgc tcgatctggc gcgcgaaggc
     5581 gggcgggtgc cggaactgcg cgattatccg gcctggcggc gcgagcgggc gcgctggttt
     5641 gcccataatg gcgcgcagga agaggcctgg ccgctgtcgg atggcacgca tctgcgcgtg
     5701 gtggcccagc cgatgcccga tggcgggctg ctgctgatcg tggaggaccg caccgagacg
     5761 ctgcgcatcg cggccatgcg cgacacgctg ctgcgcaccc gcaccgccac gttcgacagc
     5821 ctgttcgagg cggtggcggt gtttgccccc gatggccgga tgcagctgtg gaaccgccgc
     5881 tttgccagcg cctgggggct ggagagcgag ctgctcgaca gccacccgca tatcggcgat
     5941 ctgctgaagg cggtggcgcc ggggctggcg caggcggaag aggcgcgcgc gctgggcgat
     6001 gtggtgcgcg cggccacgct cgaccggcgg cagaccgagg cgcggctgaa gctggctgat
     6061 ggccgggtgc tggaatttgc cggcgtgccg ctgcctgatg gcaatggcct gctcaccgtg
     6121 ctcgacatca ccgatgcgca aaaggccgag gcggccctgc gcgaacgcaa tgccgccctt
     6181 gccgaggcgg accggctgaa gacccgcttc ctcgccaata tgagctatga gttccgcacg
     6241 ccgctcacca gcatcaccgg ctttgccgag ctgctgcagg ccgggcttgg cggcgagctg
     6301 agcgagggcg ggcgcgaata tgtggcggcg atcctggcct cttcggcgcg cctggccgag
     6361 cagatcgaga atgtgctcga cctgtcgcaa agcgaggcgg ggctgctgcc gatggccgca
     6421 gaagcgatcg acctgttccc cttcatcacc gccatcgccg gcgacaggtc tgcccggctg
     6481 ggcgagaagc acctcagcct tgatctgcgc ggtgatcgca gcgccgggca ggtgcgcggc
     6541 gatccgcgcc ggctgggcaa ggcggtgggc cacctgatcg acaatgccat tgccgccagc
     6601 ccggaaggcg cgcgcatccg gctggatctc gtcaccttgc ggcaggggcc ggggccgggc
     6661 ggcgggcgca tcaggatcat catcgccgat caggggccgg gcatggattc ggccatgctg
     6721 gcccgcgcgc tgggcggcct gaagcccgat gacgagccgg gcgagggcgc gctggagcgc
     6781 cgccagggcc tgggcctgcc acttgccagc cagctgatcg aggcgcatgg cggcacgctg
     6841 gatatccatt cgcgcccggg tgccggcacc caggccatta tcgagctgcc ccgcctgtga
     6901 ccgcgcgccc tttccttgcc ctgcccgatg aagcggccac ccaaaggctg ggcgcgcgca
     6961 ttgccgcggg gcttcaggcg ggcgatgtgg tggcgctgtc gggcgatctg ggcgccggca
     7021 agacgacgct ggcccgcgct atcctgcgcg cgctgggcca tgccggcgaa gtgccatcgc
     7081 ccagctttgc catcatcgag ccctatgagc cgccggccgt gcgcctgccg gtggtgcatg
     7141 ccgatttcta tcgcctcgcc catccgcagg aggctgagga gctggggctg gaggactatc
     7201 gcgcagatgg cgcgctcatc gccgaatggc ccgagcaggc cggcggtttc acccatgagg
     7261 gcgcctgcct tgccatccac atcgaagtgg ggccagaggg gcggcgggcc attgtgcgcc
     7321 cgggcccgga ttggctaggg cggtgggatg acagcgaatc tgcctgaggg ccttgccgaa
     7381 tttcttgtcc gcgccggctg gggcggggcc gatgtggcgc cgctggcggg cgatgcctc
//
LOCUS       DASRYE010000239         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_137701,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000239 DASRYE010000000
VERSION     DASRYE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137701"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFF98_12310"
     CDS             complement(<1..241)
                     /locus_tag="VFF98_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU64459.1"
                     /translation="MTQTTRIALVDDDDDLRAAAAQTLALAGFTVDPLAGAEAALAAV
                     GPDYPGIVVTDIRMPVVDGITLFHRLRAADGELPVI"
     gene            complement(238..1986)
                     /locus_tag="VFF98_12315"
     CDS             complement(238..1986)
                     /locus_tag="VFF98_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64460.1"
                     /translation="MPRPLDLMRQYRRAILPLALAGMGLVAVLAWQAGEWTRRSYLAE
                     AHGQLAVDAGLRAAVIQSEVARFRLLPLAFAEDRDVAAAVARQPGAADRLDRRLEALA
                     RDTGAANLYLLDPAGRTLAASNYRQAQSFVGQDYAFRNYYQQARRSGMGAQFGMGTAS
                     HQPGLYEAHRTVGGGFIVVKRDFLRLEQQWAATPGLTLVTDANGIVIIASDPALRFRA
                     MQPLAPAQVARFRHELQSGDGSLRPLGMRAGADAQIVALDGAPGVRWVHTQVPAGDFG
                     WQVHRFVPVSAQIERSVHMAWALSGLSGLVVMMAAGALVLRRQRQRLRTIRLQQEVRA
                     GTEQLRREIAERQAIEARSDLLREELRQANRLATLGQVTAGIAHETAQPVAAIRSYAE
                     NGIAFLQRGDLATAEGNFAAISRLTERIGTITAQLRSFARRREDAVLAMPVSQAVEGA
                     LLVLRPRLAGLHCQRDLGRDPQVLASRVRLEQVLVNLLQNAIDALADRREATIRLTLR
                     ESEEGVYLRVADNGPGIAPDIAARLFTPFATSRASGLGLGLVIARDIMREMGGDLAWV
                     PEEQGAVFDLLLKAAP"
     gene            2185..3492
                     /locus_tag="VFF98_12320"
     CDS             2185..3492
                     /locus_tag="VFF98_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZU64461.1"
                     /translation="MHALPHAVSLPAGPRPWYRHLYVQVLVAIAAGVALGHYAPQAGA
                     AMKPLGDAFIKLVKMVITPVIFLTIVTGVAGMRDLGAVGRVAAKAMGYFLVFSTLALA
                     VGLLVANVVQPGAMLHIDPASLNAGKVSEYAAKAHDATIVGFLTGIIPDTFVSALAEG
                     NILQVLFVAILFGIALSMVGARGEKVLALMEELSEVVFRLVALLMKAAPVGAFGAMAF
                     TIGKYGIASLLGLVSLVATFYFTSALFVLVVLGLVGRVCGFSILRLLAYLKTELLLVL
                     GTSSSESGLPGLMEKLERAGCPKSVVGMVVPAGYSFNLDGTNIYMTLAALFIAQATGV
                     HLGLGEQLLLLGVAMLSSKGAAGVTGAGFITLAATLSIVPSVPVAGMALILGVDRFMS
                     ECRSLTNFIGNAVATIVVSRWEGQLDRAALDNALSGRATAPAE"
     gene            3548..>4180
                     /locus_tag="VFF98_12325"
     CDS             3548..>4180
                     /locus_tag="VFF98_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64462.1"
                     /translation="MINRITRWSLMAAALPFAHPAQAAATDAASSTADTATAATADTA
                     AAVSATPIDAGQPANVPAHGATITFGHFTATHNPAELAVSSYPSEAAGDGNATNGYNL
                     SRWAEDWSKMCDARNRRDLVDRLKCIPFNSRHSIYLTLSGEIRERVNLTTNPGLLNAE
                     GQRQEMLRLVGGADLHVGPYLRFYGEVAHGGLGGINLGSVTGTQKNDAIWL"
BASE COUNT          669 a         1471 c         1360 g          680 t
ORIGIN      
        1 tgatcaccgg cagttcgcca tcggccgcgc gcaggcggtg gaacagggtg atgccatcga
       61 ccaccggcat gcggatatcg gtgacgacaa tgcccggata atccggcccc actgccgcca
      121 gcgcagcctc cgcgccggcc agcggatcga ccgtgaagcc ggccagcgcc agcgtctggg
      181 cggcagcggc gcgcaggtcg tcgtcgtcgt ccaccagggc gatgcgggtg gtttgggtca
      241 tggggcagcc ttcagcaaca gatcgaacac cgcgccttgc tcctcgggca cccaggccag
      301 atcgccgccc atctcgcgca tgatatcgcg cgcgatgacc agccccagcc ccagccccga
      361 agcgcgcgag gtggcaaagg gggtgaacag gcgcgcggcg atatcgggcg cgatgcccgg
      421 gccattatcg gccacgcgca gatagacgcc ctcttcgctt tcccgcagcg tcaggcggat
      481 ggtggcctcg cgccggtcgg ccagcgcgtc gatggcgttt tgcagcaggt tgacgagcac
      541 ctgctccagc cgcacccggc tggccagcac ctgcggatca cgcccgagat cgcgctggca
      601 atggaggccc gccagccgcg ggcgcagcac cagcagcgcg ccttccaccg cctgcgaaac
      661 cggcatcgcc agcaccgcat cctcgcgccg gcgggcaaag ctgcgcaatt gcgcggtgat
      721 cgtgccgatc cgctcggtca gccggctgat ggcggcgaaa ttgccctcgg cggtagccag
      781 atcgccgcgc tggaggaaag cgatgccgtt ttcggcatag ctgcggatgg cggccaccgg
      841 ctgggcggtt tcatgggcga tgccggccgt cacctggccc agcgtggcca gccggttggc
      901 ctggcgcaat tcctcgcgca gcagatcgga acgggcctct atcgcctggc gctcggcgat
      961 ctcgcggcgc aattgctcgg tgccggcgcg cacttcctgc tgcaggcgga tggtgcgcag
     1021 ccgctggcgc tggcggcgca gcaccagcgc gccggccgcc atcatcacca ccagcccact
     1081 gaggcccgaa agcgcccagg ccatgtgcac agaccgctcg atctgcgcgc tgaccggcac
     1141 gaagcggtgc acctgccagc caaaatcgcc cgcgggcacc tgcgtgtgca cccagcgcac
     1201 ccccggcgcg ccatccagcg ccacgatctg tgcatcagcg cccgccctca tgcccagcgg
     1261 ccgcagcgaa ccatcgcccg attgcagctc gtggcggaag cgggccacct gcgcgggcgc
     1321 cagcggctgc atggcgcgaa agcgcagcgc cggatcgctg gcgatgatga caatgccatt
     1381 ggcatccgtc accagagtga ggcccggcgt ggccgcccat tgctgctcca gccgcagaaa
     1441 atcgcgtttg acgacgatga agccgccccc caccgtacga tgcgcctcat agaggcccgg
     1501 ctgatggctg gccgtgccca tgccgaactg cgcgcccatg ccgctgcggc gggcctgctg
     1561 gtagtagttg cggaaggcat aatcctggcc gacgaaggat tgcgcctggc ggtaattgct
     1621 ggccgccagc gtgcgcccgg ccggatcgag cagatagagg ttggccgccc ccgtatcgcg
     1681 cgccagcgct tccagccgcc ggtccagccg atcggccgcg ccgggctggc gggccaccgc
     1741 ggcggcgaca tcgcggtctt cggcaaaggc cagcggcagc aggcggaaac gcgccacttc
     1801 cgactggatg accgccgcgc gcaggcccgc atccaccgcc agctggccat gcgcctcggc
     1861 cagataggaa cgccgtgtcc attcccccgc ctgccaggcc agcacggcca ccagccccat
     1921 gcccgccagc gccagcggca ggatcgcgcg ccgatactgg cgcatcaggt caagggggcg
     1981 aggcatggca cgcttgtgca gaaagctgca cgcgatgtcc agatggcgta gaaaatcttg
     2041 caccggatgc gccatgggaa aatgcaaagt aacttaatat caatatgata gctaattcca
     2101 aaattcctgc acaccgctcg ccaccaactg ctatcaccgc tttaccgagc cggcagcaga
     2161 ccggctccga gaggaccatt gcccatgcac gccttgcccc acgccgtttc cctgcctgcc
     2221 ggcccgcgcc cctggtatcg ccatctctat gtgcaggtgc tggtggcgat tgcggccggt
     2281 gtggcgctgg gccattatgc cccgcaggcg ggcgcggcga tgaagccact gggcgatgcc
     2341 ttcatcaagc tggtgaagat ggtgatcacc ccggtgatct tcctcaccat cgtcaccggc
     2401 gttgccggca tgcgtgatct gggcgcggtg ggccgggtgg cggccaaggc gatgggctat
     2461 ttcctggtct tttccacgct ggcgctggcg gtgggtctgc tggtggccaa tgtcgtccag
     2521 ccgggggcca tgctgcatat cgatccggcc agcctcaacg ccggcaaggt cagcgaatat
     2581 gccgccaagg cgcatgatgc cacgatcgtc ggctttctca ccggcatcat ccccgacact
     2641 ttcgtctcgg cgctggccga gggcaatatc ctgcaggtgc tgttcgtggc gatcctgttc
     2701 ggcattgcgc tttcgatggt cggcgcgcgc ggcgagaagg tgctggcgct gatggaggaa
     2761 ttgtcggaag tcgtcttccg cctggtggcc cttctgatga aggcggcgcc ggtgggcgcg
     2821 tttggcgcca tggccttcac cattggcaaa tacggcattg ccagcctgct gggcctcgtc
     2881 agcctggtcg ccacgttcta cttcacctcg gcgctgttcg tgctggtggt gctggggctg
     2941 gtggggcgcg tgtgcggctt ctcgatcctg cggctgctgg cctacctcaa gaccgaactg
     3001 ctgctggtgc tgggcacctc ttcttccgaa agcggcctgc ccggcctgat ggagaagctg
     3061 gagcgcgccg gctgccccaa gtcggtggtg ggcatggtgg tgccggcggg ctattccttc
     3121 aaccttgatg gcaccaacat ctacatgacg ctggccgccc tgttcattgc ccaggccacc
     3181 ggcgtgcacc tgggccttgg cgaacagctg ctgctgctgg gcgttgccat gctctcctcg
     3241 aagggcgcgg cgggcgttac cggggccggc ttcatcaccc ttgccgccac cctttccatc
     3301 gtgccttcgg tgccggtggc gggcatggcg ctgatcctgg gcgtggaccg cttcatgagc
     3361 gaatgccgca gcctcaccaa tttcatcggc aatgcggtcg ccacgatcgt cgtctcgcgc
     3421 tgggaaggcc agctcgatcg cgccgcgctc gacaacgccc tttccggccg cgccaccgcg
     3481 ccggcggaat aatccgcgcc cttcggggcg ccccataaca acaacaccaa actcagggat
     3541 acaggatatg atcaaccgca tcacgcggtg gtcgctgatg gcggccgccc tccccttcgc
     3601 gcaccctgcg caggcagccg ccacggacgc cgccagcagc accgccgata ccgccaccgc
     3661 cgccactgcc gatacggctg ccgccgtcag cgccacgccc atcgatgccg gccagcccgc
     3721 caatgtgcct gctcatggcg ccaccatcac cttcggccat ttcaccgcca cgcataatcc
     3781 ggcggaactg gccgtctcct cctatccgtc ggaagcggcg ggcgatggca atgccaccaa
     3841 tggctacaac ctgtcgcgct gggcggaaga ctggtcgaag atgtgcgatg cgcgtaaccg
     3901 gcgcgatctg gtcgaccggc tgaagtgcat ccccttcaac agccgccaca gcatctacct
     3961 caccctcagc ggcgaaatcc gcgagcgcgt caacctcacc accaaccccg gcctgctgaa
     4021 cgccgagggc cagcgccagg aaatgctgcg gctggtgggc ggcgcggatc tgcatgtggg
     4081 gccctatctg cgcttctatg gcgaggtggc ccatggcggg ctgggcggca tcaacctggg
     4141 ctctgtcacc ggcacgcaga agaacgacgc gatctggctg
//
LOCUS       DASRYE010000240         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_85178,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000240 DASRYE010000000
VERSION     DASRYE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85178"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFF98_12330"
     CDS             complement(<1..249)
                     /locus_tag="VFF98_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU64463.1"
                     /translation="MTLPDVPPLALVARLAGKRCFVTGGASGLGAAIARRFVAEGAAE
                     VVIADIDAVAGRALAAELGAAAHFIALDVTSEASWQTAL"
     gene            399..815
                     /locus_tag="VFF98_12335"
     CDS             399..815
                     /locus_tag="VFF98_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU64464.1"
                     /translation="MTLTPAQIEQFVDDLYAATAVGDFDKAADMLTDDFFVTEADNLP
                     MAGIYRGRNALKELFTKVMGLMDVAGLERVQTLVGGDYAVTYLSFRFADPALKSAELC
                     ELFRFRDGKCCEIKPFYFDPATVHAAVAAKQAALTA"
     gene            934..2565
                     /locus_tag="VFF98_12340"
     CDS             934..2565
                     /locus_tag="VFF98_12340"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HZU64465.1"
                     /translation="MRIWQDLGTGLAALAVVLAGGAAPSALAQAGAANTPPAPDYADI
                     ADWAAGPAGFGPSAVLPAGASKPVKYPRVDVFYVHPTVFSSETALNQDLADAQANRWV
                     DEGAVAREASAFTSCCRVFAPRYRAASFQALAGDRRDAAFALAYADVESAFGYYLAHF
                     NKGRPFILAGDGQGASLLAALLDRRFEGTVLRDQLVAAYLVGGNVAQGDFGRRWHGLP
                     ACDEARQTGCVVQWNVVAAGSNLPALAAPHEQAYTARTGDVAGQRLLCTNPLTFEANR
                     PDASAGAARGAIPGAPGYGAPAGLEARAVAARCEHGLLVVSPDAALKARAAGDGSLGQ
                     YGYTLFYQDIRENAALRAAAWRPGQAGVASVAAPVPVVAPAPAPAPAPELVAAPARPA
                     DPAAAEAGAHLLFDAGAARPAPVAAAPALAVVPEAAPTPVVKVAPAVVAPKPVAAPKP
                     VPVAEPAPLVAARPVLPQAAPAPAPVAAPAPVAVPVAAVPVSAPVPALVAPAVSAQAG
                     PAAPAQADNGDQAKVAPGKPRRGHRLGRFFHSSEH"
     gene            complement(2614..>3136)
                     /locus_tag="VFF98_12345"
     CDS             complement(2614..>3136)
                     /locus_tag="VFF98_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZU64466.1"
                     /translation="PPVAAALQSALRLREKAHKAGVPVILTNVVYQAGGVDGGRFFEK
                     AAPLHYFVQGHPMAAWGPGLVPQEGEIIVSKQYPSAFFGTSLASTLTALGVDSVLLTG
                     LTTSGCVRASCVDAMSHGFRTAVIAEACGDRHSAPHEANLFDMNAKYADVVSEEEALA
                     FFKGLKKKAKARR"
BASE COUNT          432 a         1047 c         1142 g          515 t
ORIGIN      
        1 cagcgcggtc tgccagctgg cctcgctggt gacatcaagg gcgatgaagt gggccgccgc
       61 gcccagctct gcggccagcg cccggccggc cacggcatcg atatcggcga tcaccacttc
      121 ggcggcgccc tctgccacga aacggcgcgc gatcgccgcg ccaaggccag aggccccgcc
      181 ggtgacgaaa cagcgcttgc cagccagcct tgccacaagc gcaaggggcg gaacatcggg
      241 aagggtcatc gtgcagctct ccgcgccggc ccttctggcg gggccgccat ctgcctgctc
      301 taacccggct ttgggtaaac gccggctagt tatatcgcta ggttcgccgc ctgcggtttc
      361 ggcggaaaat cgggagaaag attgagagga gcttccccat gacgctgacg cctgcccaga
      421 tcgagcaatt tgtcgatgat ctttatgccg ccaccgcggt cggcgatttc gacaaggcgg
      481 ccgacatgct gaccgacgat ttcttcgtga ccgaggccga caacctgccg atggccggca
      541 tctatcgtgg ccgcaatgcg ctgaaggagc tgttcaccaa ggtgatgggc cttatggatg
      601 tggccgggct ggagcgcgtg cagacgctgg tgggcggcga ttacgcggtc acctatcttt
      661 ccttccgctt tgccgatccg gcgctcaagt ctgccgagct gtgcgagctg ttccgcttcc
      721 gcgatggcaa gtgctgcgag atcaagccct tctatttcga tccggccacc gtgcatgcgg
      781 ccgtggcggc caagcaggcg gcgttgacgg cctgaggggc tctggcgggg cggcgcctca
      841 aatcgcgctg gaaactcgcg ctgcgcctgc tatcgaaggc gccgggtgcg caggtgatgc
      901 gctctggcgc cgatggcagg aacaggaacg acaatgcgga tctggcagga tttgggcacc
      961 ggccttgcgg cgctggccgt ggttttggca gggggcgccg cgccttcggc cctggcgcag
     1021 gcgggcgccg ccaacacccc gcccgcgccc gattatgccg acattgccga ctgggcggcg
     1081 ggccctgcgg gctttggccc ttcggcggtg ctgccggcag gggccagcaa gccggtgaaa
     1141 tatccgcggg tcgatgtgtt ctatgtccac ccgacagtgt tcagctccga gactgccctc
     1201 aaccaggatc tggctgatgc gcaggccaac cgctgggtgg atgaaggcgc cgtggcgcgc
     1261 gaggccagcg ctttcacctc gtgctgccgg gtgtttgccc cgcgctatcg ggcggcctcg
     1321 ttccaggcgc tggcggggga ccggcgcgat gcggcctttg cccttgctta tgccgatgtg
     1381 gaaagcgcct tcggttatta cctggcccat ttcaacaagg gccgcccttt catcctcgct
     1441 ggtgacgggc agggggccag cctgctggca gcgctgctcg accggcggtt cgagggcacg
     1501 gtgctgcgcg atcagctggt ggcggcctat ctggtgggcg gcaatgtggc gcagggcgat
     1561 ttcggccggc gctggcatgg cctgcccgct tgcgacgagg cccgccagac gggctgcgtg
     1621 gtgcagtgga atgtggtggc cgcgggcagc aacctgccgg cgctggccgc cccgcatgag
     1681 caggcctata ccgcgcgcac cggcgatgtg gccggccagc gcctgctgtg caccaacccg
     1741 ctgaccttcg aggccaaccg gccggatgcg tctgcgggcg ctgcgcgcgg cgccattccc
     1801 ggcgcgcccg gctatggtgc gccggccggg ctggaagccc gcgcggtggc ggcgcgctgc
     1861 gaacatggcc tgctggtggt gagcccggat gccgccctga aggcgcgggc ggcaggcgat
     1921 ggctcgctgg ggcaatatgg ctacacgctg ttctatcagg acatccgcga gaatgccgcg
     1981 ctgcgggcgg cggcctggcg gccggggcag gccggggtgg ccagtgtggc agcgcctgtg
     2041 ccggtggtag cgcccgcgcc ggcgcccgcc cccgcccccg agctggtggc agcgcccgcc
     2101 cggccggccg atccggcagc ggcggaggca ggcgcgcatc tgttgttcga tgcgggcgcc
     2161 gcgcggcccg cgccggttgc tgcggcgcct gccttggctg tcgtgccgga agcagcgccg
     2221 acgccggtgg tgaaggtggc gccggcagtg gtggcgccaa agccggtggc cgcgccaaag
     2281 cctgtgccgg ttgccgagcc ggctccgctg gttgcggcgc ggccggtgtt gccgcaagcc
     2341 gcgccggcac ctgctcctgt tgctgcgccc gcgcctgttg ctgtgccggt ggcggcagtg
     2401 ccggtcagcg cgccggtgcc cgccctggtg gcgccagcgg tgtcggccca ggcgggccct
     2461 gccgcgcctg cccaggctga caatggcgat caggccaagg tggcgccggg caagccgcgc
     2521 cgtggccacc ggctgggccg cttcttccac agctcagagc attgagggcc tgaacggggt
     2581 gccgggcgca aaaaggcccg gcgcctgccc catttagcgg cgggctttgg cctttttctt
     2641 caggcccttg aaaaaagcga gagcctcttc ctcgctcacc acatcggcat atttggcgtt
     2701 catgtcgaag aggttggcct catgcggggc tgaatggcgg tcgccgcagg cctcggcgat
     2761 aacggcggtg cgaaagccgt ggctcatcgc atcgacgcag gaagcgcgca cgcagcccga
     2821 tgtggtgagg cccgtcagca gcacgctgtc cacccccagc gcggtcagcg tgctggccag
     2881 cgaagtgccg aaaaaggccg agggatattg cttggagacg atgatctcgc cctcctgcgg
     2941 caccagcccg gggccccagg cggccatcgg gtggccttgc acgaaatagt gcaagggcgc
     3001 tgccttctcg aagaagcgcc cgccatccac gccgcctgcc tggtagacga cattggtgag
     3061 gatcaccggc acgccggcct tgtgggcctt ttcgcgcagg cgcagcgccg attgcagggc
     3121 cgctgccacc ggcggg
//
LOCUS       DASRYE010000241         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_208641,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000241 DASRYE010000000
VERSION     DASRYE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208641"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFF98_12350"
     CDS             <1..214
                     /locus_tag="VFF98_12350"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544237.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZU64467.1"
                     /translation="LGAHVLTLASDTGLTDAGLKIRTLRLPDTFQDHDNPDKQYDEAG
                     LTAPHIVNTVLTALRWNEGALSAAVR"
     gene            complement(228..2015)
                     /locus_tag="VFF98_12355"
     CDS             complement(228..2015)
                     /locus_tag="VFF98_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HZU64468.1"
                     /translation="MTTSAATPAPAAGWFGHPAPLARLFGIEMWERFGYYGMRALLTL
                     YLTKHVLLADRAASGLYGGYSALVYLTPLVGGLVADQLLGAKRAVRFGAALMALGYGL
                     LALGAATSGGQARAWLDFDGARHPITIAHHHDGPTDTGAEARQVLVAGHPLTIKGHAD
                     GSIALVAADGQVARQLPAGAFAEGASQSPAMLALLLLALSTLAVGNGFFKPNISTMVG
                     ALYAGTDSRRDAGFTIFYMGINIGSILGQALCPLFADTIGWAAGFAVAGGGMALAFAM
                     VALAGDRLAGLGERPAQANPRRDRWVYLGAIATIGLFTLLYRNLMASPAPVAGAGLGG
                     YLLALPVMGKLLIGTFLLGVPALLAWTWRQGSRAEFEMALAAMALVTFNIVFWTLFEQ
                     AGTSLTLFADRNTDRSVFGLFELSAPQTQNFNPIAIVLLAPLMSALWGWLGRRGWEPS
                     IPVKFGIALLGAGAGFLLLVLGGRFAGPHAQVGLAWLAGLYVIHSVAELCISPVGLSM
                     ITKLAIARVVGLMMGVWFLSISVAQYAAGMVAQMASVATVGGEVMDPAQSLATYLAVF
                     TRIGGVAIALGLALLALARPLGRLTHGVR"
     gene            2368..2646
                     /locus_tag="VFF98_12360"
     CDS             2368..2646
                     /locus_tag="VFF98_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HZU64469.1"
                     /translation="MIRSELLQILSRDNPELRADDVERIVDVFFDEIAQRLSDGGRVE
                     LRGFGAFSTRERDPRKGRNPRTGETVEVPGKRVPFFKPGKEIRLRLNA"
     gene            2738..2821
                     /locus_tag="VFF98_12365"
     tRNA            2738..2821
                     /locus_tag="VFF98_12365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2771..2773,aa:Leu,seq:taa)
     gene            2861..3337
                     /gene="ybaK"
                     /locus_tag="VFF98_12370"
     CDS             2861..3337
                     /gene="ybaK"
                     /locus_tag="VFF98_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HZU64470.1"
                     /translation="MAKDTRATRMLAEAGIAFTRHTYDYDADADSIGLAAAAALGADP
                     ACVLKTLMVLVDGKPAVAIVPSDCEVAMKKLAAALGGKAAQMMAPAAAERLAGYKVGG
                     ISPFGQTRRLPVVMEAAAMAAPLVFLNGGQRGLQVQLAPAEAARLLAAVVAPIVAG"
     gene            3449..>4667
                     /locus_tag="VFF98_12375"
     CDS             3449..>4667
                     /locus_tag="VFF98_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZU64471.1"
                     /translation="MSEISTVAGQAPAAPDVSIIMPCLNEIISLPHCIVNAQAALALV
                     RERYGLTGEIVIADNGSTDGSQALAQSLGARVVPVARRGYGAALIGGCEGAHGRFLLM
                     GDADGSYDFTDGVAMIGRLVAGADLCMGSRFAGGIAPGAMPWKNRYIGNPALTGVLNL
                     FFRSGIEDAHCGIRAITRAALLACGLSSSGMEFASEMVVKASLLKLRIEEVPATLSPD
                     LRQRAPHLRPWRDGWRHLRYLLMLSPRWLFGVPGALAMLIGLALLGAGGADYLVPGTL
                     PQIGAYWTLLGDTLAALGHLSLLMALAGHLYAVRAGYRLPGRITRVAGRFASLETMLV
                     SGLGLMGLGIAVLAAVAYGWVARDFQKADSLWPAAVGCLAVQLGLQNALGGFLMAIIG
                     GHEARFFAGLDSRP"
BASE COUNT          750 a         1546 c         1694 g          677 t
ORIGIN      
        1 tctgggcgcc catgtgctga cgctggcctc cgacaccggc cttaccgacg ccggcctcaa
       61 gatccgcacc ctgcgcctgc ccgacacctt ccaggaccac gacaaccccg acaagcaata
      121 cgacgaagcc ggcctcaccg ctccccacat cgtcaacacc gtcctcaccg ccctcaggtg
      181 gaacgagggg gcgctgtctg ccgcagtgcg ctgaggggcg ctgattttca gcgcacgccg
      241 tgggtcagcc ggcccagcgg ccgggccagc gccagcagcg ccagccccag cgcgatggcc
      301 acgccgccga tgcgggtgaa aacggccagg taggtggcca ggctttgcgc cgggtccatc
      361 acctcgccgc ccacggtggc aacgctggcc atctgcgcca ccatgcccgc ggcatattgc
      421 gccaccgaga tcgacaggaa ccacacgccc atcatcagcc ccaccacgcg ggcgatggcc
      481 agcttggtga tcatcgacag gccgaccggc gagatgcaca gctctgccac cgaatggatg
      541 acatagagcc ccgccagcca cgccagcccc acctgcgcat ggggcccggc aaagcgcccg
      601 cccagcacca gcagcaggaa gccggcgccc gcccccagca aggcaatgcc gaatttgacc
      661 gggatcgaag gttcccaccc gcgccggccc agccagcccc acagggcgct catcaagggg
      721 gccagcagca cgatcgcaat cgggttgaaa ttctgcgtct gcggggcgga aagctcgaac
      781 aggccgaaga ccgagcgatc ggtgttgcga tcggcaaaca gggtgagcga ggtgcccgcc
      841 tgctcgaaca gcgtccagaa gacgatgttg aatgtgacca gcgccatggc cgccagcgcc
      901 atctcgaatt cggcgcggct gccctgccgc caggtccagg ccagcagcgc cggcacgccc
      961 agcaggaagg tgccaatcag cagcttgccc atcaccggca gcgccagcag atagccgcca
     1021 aggccggcgc cggccaccgg cgcggggctg gccatcaggt tgcgatagag cagggtgaac
     1081 aggccgatgg tggcgatagc gcccagatag acccagcggt cgcgccgggg gttggcctgt
     1141 gcgggccgct cgccaaggcc ggcgagcctg tctccggcca gcgccaccat ggcaaaggcc
     1201 agcgccatgc cgccgcccgc cacggcaaag cccgccgccc agccgatcgt gtcggcaaac
     1261 agcgggcaca gcgcctggcc caggatcgag ccgatgttga tgcccatgta gaagatggtg
     1321 aagccggcat cgcggcggct gtcggtgccg gcatacagcg cgccgaccat ggtggagatg
     1381 ttgggcttga agaagccgtt gcccaccgcc agcgtggaca gcgccagcag cagcagcgcc
     1441 agcatcgccg ggctctggct ggcgccttcg gcaaaggcgc cggcgggcaa ttggcgggcc
     1501 acctggccat ctgcggcgac aagcgcgatg ctgccatcgg catggccctt gatagtgagc
     1561 gggtggccgg ccaccagcac ctgccgggcc tcggcgccgg tgtcggtggg gccatcgtgg
     1621 tggtgggcga tggtgatcgg gtggcgggcg ccgtcgaaat ccagccaggc ccgggcctgg
     1681 ccgcccgatg tggccgcccc cagcgccagc aggccatagc ccagcgccat cagcgccgcg
     1741 ccaaagcgca ctgcccgctt ggcccccagc agctgatcgg ccaccagccc gcccaccagc
     1801 ggggtgagat agaccagcgc cgaatagccg ccatagaggc ccgaggcggc gcgatcggcc
     1861 agcagcacat gtttggtgag atagagcgtg agcagcgcgc gcatgccgta atagccaaag
     1921 cgctcccaca tctcgatgcc gaacaggcgg gccagcggcg cggggtggcc aaaccagccg
     1981 gcggcggggg caggcgtagc ggcagaggta gtcatgcgtg ggcggctccg gcaggcgtga
     2041 tggccatggc gtagcggcgg ggggcggggc gagacaacgg aattttcgcg caaaaaggcg
     2101 cgcaggtgcc agcggcgggg gcggggcccc ggtgggggat acgtatcaat gcgtatttcc
     2161 cgccgaaaac cgcaccgatt cggcacaaac cctcgttcag cttgccgcaa acccgcaggt
     2221 ggcattgcac aaaccgttga actctggcac ttatgatcgg tgctggaacg gcgttgacaa
     2281 gccgatcagc cgggagagcg gccgcgccgg cagacgccgg gcgcaagcgg tttgaagggc
     2341 cgttcgggat gctacggggg atatgggatg atcaggtcag aactgctgca gatactctcg
     2401 cgggataatc cggaactgcg cgccgacgat gtggagcgca ttgtcgatgt gtttttcgat
     2461 gaaatcgccc agcgcctgtc ggatggcggc cgggtggagc tgcgcggctt cggcgccttt
     2521 tccacgcggg agcgtgatcc gcgcaagggc cgcaacccgc gcaccggcga aaccgtggaa
     2581 gtgccgggca agcgcgtgcc cttcttcaag ccgggcaagg aaatccgcct gcgcctcaac
     2641 gcctgagggc agggcgccag cgccgcgcca agcgcctgct cgggggagcg ggcgcttgcc
     2701 ggcggcggcc cgatctgcca caggcgctgc ttccggcgcg gacgtggcgg aatggtagac
     2761 gcaggggact taaaatccct tgagcttgct cgtgcgggtt cgagtcccgc cgtccgtacc
     2821 atttgccctg ccgggggcgc ccccggcagg aacgccagcc atggccaagg atacccgcgc
     2881 aacccgcatg ctggcagagg ccggcatcgc cttcacccgc cacacctacg actatgacgc
     2941 cgatgccgac agcattggcc tggccgccgc cgcagcgctt ggggcggatc cggcctgtgt
     3001 gctgaaaacg ctgatggtgc tggtggatgg caagccggcg gtggcgatcg tgcccagcga
     3061 ttgcgaagtg gcgatgaaga agctggcggc ggcgctgggc ggcaaggcgg cgcagatgat
     3121 ggcgcccgcc gctgccgagc gcctggccgg ctacaaggtg ggcggcatca gcccctttgg
     3181 ccagacccgg cgcctgccgg tggtgatgga ggccgcggcc atggccgcgc cgctggtgtt
     3241 cctcaacggc gggcagcggg ggctgcaggt gcagctggcg ccggcagagg ccgcccggct
     3301 gctggcggca gtggtggcgc cgatcgtcgc cggatagcgg ccgcgctggc tggcggcgcc
     3361 ggcaacgccc aaatccgcgc agcccatttg cactcggcca gccatgccgc taggcggggg
     3421 gcgggcgcaa atcatggggc atggcggcgt gagcgaaatt tccacagtgg cagggcaggc
     3481 cccggcagcg cctgatgtgt cgatcatcat gccctgcctg aacgagatca tcagcctgcc
     3541 gcattgcatc gtcaatgcgc aggccgcgct ggcgctggtg cgcgagcgct atggcctgac
     3601 aggcgagatc gtcattgccg acaatggctc caccgatggc agccaggcgc tggcgcaaag
     3661 cctgggcgcg cgggtggtgc cggtggcgcg gcgcggctat ggcgcggcgc tgatcggcgg
     3721 ctgcgaaggg gcccatggcc gcttcctgct gatgggcgat gccgatggca gctatgattt
     3781 caccgatggc gtggcgatga tcggccggct ggtggcgggc gcggatctgt gcatggggtc
     3841 gcgctttgcc ggcggcattg ccccgggcgc catgccctgg aagaaccgct atatcggcaa
     3901 tccggcgctc acgggcgtgc tcaacctgtt tttccgaagc ggcattgagg atgcccattg
     3961 cggcatccgc gccatcaccc gcgcggcgtt actggcttgc ggcctgtctt ccagcggcat
     4021 ggaatttgcc agcgagatgg tggtgaaggc cagcctcctc aagctgcgca tcgaggaagt
     4081 gccggccacc ctctcgccag acctgcgcca gcgcgcgccg catctgcgcc cctggcgcga
     4141 tggctggcgg catttgcgct atctgctgat gctgagcccg cgctggctgt ttggcgtgcc
     4201 cggcgcgctg gccatgctga tcgggctggc gctgctgggg gcgggcgggg ccgattatct
     4261 ggtgcccggc acgctgccgc agatcggcgc ctactggacc ttgctgggcg atacgctggc
     4321 ggctttgggc catctgtcgc tgctgatggc gctggcgggg catttgtatg cggtgcgggc
     4381 gggctatcgg ctgccggggc ggatcacgcg ggtggccggg cgctttgcca gcctggagac
     4441 gatgctcgtc tcggggctgg ggctgatggg gctgggcatc gcggtgctgg cggcggtggc
     4501 ctatggctgg gtggcgcgcg atttccagaa ggcggattcg ctgtggccgg cggcggtggg
     4561 ctgcctggcc gtgcagctgg gcctgcagaa cgcgctgggt ggcttcctga tggcgatcat
     4621 cggcgggcat gaggcgcgct tctttgccgg gctggacagc cggccgg
//
LOCUS       DASRYE010000242        16199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_43404,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000242 DASRYE010000000
VERSION     DASRYE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16199
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43404"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFF98_12380"
     CDS             complement(<1..235)
                     /locus_tag="VFF98_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU64472.1"
                     /translation="MGLPLSHWSFEGLSVGCRIGGAGLPLLLIHGSGPGASTIGNWRL
                     VLEDLANRYHVHAMDLVGFGESDRKPAPPYFDFE"
     gene            complement(244..651)
                     /locus_tag="VFF98_12385"
     CDS             complement(244..651)
                     /locus_tag="VFF98_12385"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZU64473.1"
                     /translation="MADIQIYNPKTLGTPMGQYSQVTRVKANEFLFIAGQLAGDAQDN
                     IIGEGDFDAQCEQVFRNIHAALLDAGADWGNVVQFTTYLVHSQDIPRFMDFRLRRFPQ
                     FFPDGVYPPNTLLMIDRLVKEPFLVEVQTIAAL"
     gene            complement(697..1389)
                     /locus_tag="VFF98_12390"
     CDS             complement(697..1389)
                     /locus_tag="VFF98_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU64474.1"
                     /translation="MLETDNSSLPETLYQAVREAILDGRFAPGAELRQEVLARQYGVS
                     RVPLREALSRLQADGLIELRPRRGFAVAELDVVEIIELFELRMVVEQHALEVAARMRS
                     EADLREIEALLVQMEEIEAHTPGNFPEWLDLNRRFHHRLVACAGRERLAATVSNLRDA
                     TEPYVRLEAHATGHVEDASSEHRQLYDAVRERNATLAGEVAREHALGTMKRLVEWLRL
                     RKQVGKRGKSAA"
     gene            1626..2900
                     /locus_tag="VFF98_12395"
     CDS             1626..2900
                     /locus_tag="VFF98_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU64475.1"
                     /translation="MTTNAAIQWPAEDCSRIPFAIYHDPAVYEREQEAIFRGPTWNYI
                     GLEAELPNKGDFITTRIGATPILVSRDKTGAVKAFINRCAHRGAELRREPCGNAQLHV
                     CVYHQWSFELDGRLRGVPFPSGVNGVGGLPRDFDKKAIRLHELTVRSYKGVLFATFDA
                     TGESLEDYLGEAICGELDRMFARPIKILGYQRQRMNGNWKLYNDNVRDPNHGGLLHMF
                     HATFGLYRLSQIGGARMDPKGRHNITYNQLDSDDVAASDEGYKDTQKVYSKGYKLEDM
                     RMLSNRTEFPDAVTLVIASVFPNSVFQQIANSLCTRQIRTYGVDEMELYWTYFGYTDD
                     SEEMTRHRLLQSNLCGPGGYISMEDGEAVEIVHRATARETDAHAICEIGGKGPIVDQP
                     NLVSEVPVRGFYGYYTDLMGFAKGPQAQEALA"
     gene            2897..3373
                     /locus_tag="VFF98_12400"
     CDS             2897..3373
                     /locus_tag="VFF98_12400"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZU64476.1"
                     /translation="MSVSMEQRLAALDLIARAANSLDADRMEDWAECFFEDGSYQILS
                     RENVERGLPLPLFMAENKQMIRDRVLSLRKANVTNPHRDRHIIGPAEMRAGADGEVVS
                     EAGFAVYSTTPEGVSRLFSLGVYKDRIQIGDAGARFVERTAIVDMFSIPTMLSTPV"
     gene            3470..5641
                     /locus_tag="VFF98_12405"
     CDS             3470..5641
                     /locus_tag="VFF98_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64477.1"
                     /translation="MRIHKINRTLLITACSTLALIASPACAQTGAAPAPTKADGLGEI
                     VVTAERHDSTIQKTPIAIAAYSAAQLEREGVTSLAGLTAIAPNLGFATVAGQSIVTIR
                     GISSRDTSEVGDPAVAVSTDGFYNNRPYGLNASLFDLERVEVLRGPQGTLNGRNAVGG
                     AINVVTAKPTKQFAADVSLGYGNYNAIEARGMVNVPVSDRLQLRAAILSTSHDGYRNN
                     APAANGDDEDTKAGRLAIAFQPTDRFSGLVELEMIKMGGAGQSLLNVPFSYTGSAVNH
                     NVAAPTASAAGWASYKSGVLDMNQKTLRWQFDYAAGPVKITYLGGFQSLYWHATADLS
                     LAGTPRQFVQIEQPSTQNHELRFASATGGRFTWQAGVFYFREDSSLHSYNEYTSAAGV
                     YPGFAYYYGPIATQSVAGYGQASYKLTDALTLTGGLRYTDDSKSRSGGYYIPNGTTNP
                     VSYTGYTGTKSGHWSKLTYHAALDWQASARNMLYAKVDTGYKAGGFDDVGTYQPETVT
                     AFEIGAKNRFWGNKLELNLSAFYDDYSNQQVSQLIQFGDNAGATHVQNAGKSHIYGIE
                     ADSVAMLSPDDRLNLTVDWLHAKFVQFIGSTPDSTANVNFAGNALPQSPVWTIAAGYE
                     HGFDVAHGRLTARAETKFTSSQYYEYYNYADMRQGAYTNTTLSLAYAPASKGWQVEAY
                     VHNLENNSTLAYAEENYIVNTYRYAFNPPRTYGIRFSAHIK"
     gene            5715..8078
                     /locus_tag="VFF98_12410"
     CDS             5715..8078
                     /locus_tag="VFF98_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZU64478.1"
                     /translation="MTDTPPAAPATHLAQRVAALTDAEKAELLTGQGMWRSHAIGRLG
                     IPALLMTDGAYGVRYSISQIDGQQQGGQDLDQFLAVVNTRARDMKAAFGNTKPATCYP
                     NGSAIACSWDVATAYQLGASLAAECRAFDVNILLGPGINIRRTPLGGRGYEYYAEDPV
                     LTGDFAAAVVEGLQDHGVGASLKHFACNNSEIERTTMDSRVSPRALREIYLLGFERVV
                     RKARPWTVMSSYNRLNGMQTAQDPWLLTELLRGEWGFDGVVVSDWHGIKDRPAALAAG
                     NDLDMPQNASRQRALAEAIADGSLPAEVVNQACARMLALVDKAKAAAGPVVPFDAAEH
                     HAHARAMAAASIVLLRNEGGLLPLQPAALRRILVVGDGAVNPVIQGSGSATTLPTARD
                     IPLDEIARQAGAGVIVQHLPGHGDTPEETAARTAEAAKAAEGADAVLVFVTTDAAEDG
                     EGADRRTLALARGHDALIEALAAASGKVVVVLANPDAVVMPWAGKVAAIVETFFAGQA
                     MGGALADILFGAVNPSGKLTVSFPARIEDTPAYLSYPGEAGVHEYAEGIFVGYRYYDK
                     RAIAPLFPFGFGLSYTSFAYSDLEVATPALAEGEDLRIAFTLANTGPVAGAEVAQLYL
                     GFPESRVQRPLRELKGFAKRMLAPGETTRVELAVPARDLCFFDAEAGRWRLEEGAIAI
                     ELGASSRDIRLAGAARAQPARPHYPPLAIDSQPTKVLANPVARAHFERFLAEKLGLSP
                     DQAARMLAYTRSSFFGIHTTLNYFFRQDLPRAEIAAIIAAINAETAA"
     gene            8102..8806
                     /locus_tag="VFF98_12415"
     CDS             8102..8806
                     /locus_tag="VFF98_12415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZU64479.1"
                     /translation="MTSPLPPIAAYVYGEKKPVPEALRGFITHADTAVVSIDMHLGHL
                     SDKPECPCPAPRARAIIDPVNAFHRAARDLGIPVIHVRSVLRRTGEDDLKGLAPAAWR
                     SLVELHGPAIPNIAEHAIEGSEWTQFATEVLDSDLIVETKRRLSPFYPTDLDFLLRNM
                     GVRRVVFNGGMTDCCVLNATFDASNRGYRVVVARDLVRGSNAEMEDAALKMVSLHTGL
                     VMEADDILTQWRATRP"
     gene            8832..9833
                     /locus_tag="VFF98_12420"
     CDS             8832..9833
                     /locus_tag="VFF98_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64480.1"
                     /translation="MIDIVGGISMSHVPGALGWPDAPPADQQARLAAIHTHLRERLAA
                     ARPDLIVAFLDDHFENHFRNLLPTLGVGIAPAHIGPAAYMMEALRFDRQHAIPSDADL
                     AEGLLRELVHAGFDAARMGAIEYGNNLMMPLHYIRPEFDIPVLPVFINVFSPPLMPYG
                     RAYDLGLATRRALEAIGGDRRILFLATGGLSHWPPVWTEGAPEDDSFLQRMKTYQTEG
                     KGVIADDPDLYTDLARYEMKMMDKMQWPLGYQHPLVNAEFDEELLARFAEGDVRYMRE
                     ITYDEVEQRGGHGGHELLNWVALMGAMNGARADYTAYEPVTEWITGMAYAAYDGQQG"
     gene            complement(9893..10963)
                     /locus_tag="VFF98_12425"
     CDS             complement(9893..10963)
                     /locus_tag="VFF98_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64481.1"
                     /translation="MSILGVESVVFGVDDVAEHARFWSDFGLPVERIAADEAVFRLAS
                     GSRVILYAHGDARLPGPDPFAGNGVKETVWGVDTAENLERIAASLASEVAITRDADGT
                     VHCVCPDGQPIALRVWAKRPVASETSPVNTPGNHPRFNQHRIWRHKAIPKTINHVVFF
                     SPDYVGAFEFYERHLGFRYVDHSKGVGIFSRAGGTFEHHSIFWVNCDLPFAPDAFKFM
                     HIAFGMDDIDEVMLGANLMAEKGWKNESMNSSGGISRHRISSAIYYYCDIPGKGGEAE
                     YHADTDYLDDNWVPRAWDFRFGSLLWSNNAPPIFRGPDIPWDMAFDADRKSFEPYRKA
                     KPGKQPVDGHLAAITEDDEHAI"
     gene            complement(11098..12249)
                     /locus_tag="VFF98_12430"
     CDS             complement(11098..12249)
                     /locus_tag="VFF98_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU64482.1"
                     /translation="MFEPFPGNYVWNLATNLALVCGGNHGEIDEACRPVREASKAGAD
                     AGSALLFDSWIAVADQVAQNAEDDLAAGYRLSAGTKFLRASGYYLAAERMQSRDYAPR
                     WVAYDKGLDLYHKGSALRGLHVDKVEIPYEGSAYTAIFVHDGSGTPRPTLVSVNGLDS
                     MKEQVNMAGHGQSNLERGMNTLFLDQPGTGEAIRKRGLPAVYDAERWGSPAFDYLLTR
                     SDVIHSQIGIFGLSFGGYHAPRIAANDPRYALCAVMGANHVWGQRQRERVKNEGENPV
                     PHYWDHVLWVFGYDDREAFLAYADQITLNGQVEKIRVPFLITHGENDRQIPAFNAQLS
                     YDQAVNSPNRHLRMFTKADFEVEHCGADNGTGMRDYIADWCAQTFAAMS"
     gene            complement(12261..13046)
                     /locus_tag="VFF98_12435"
     CDS             complement(12261..13046)
                     /locus_tag="VFF98_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64483.1"
                     /translation="MSEGGMSESLPRTPGFRLDGQRALVTGAGRGIGLACAAALAEAG
                     AHVVLAARTAAEIEAGAGAIRAAGGSAQAAVLDVSDLAGVEAFFAGQPAFHVLVNNAG
                     TNRPKPMWEVSPEDYDAVLDLNVKSAFFVAQGAARAMIREGIAGSLIHMGSQMGHVGG
                     PRRSLYCASKWAIEGMNKAFALDLAPHGIRSNTIAPTFIETPMTKPFFEDEAFRASVL
                     AKIKLGRLGRVEDLMGSVLFLASPASALITGTSLIVDGGWTAD"
     gene            complement(13043..14374)
                     /gene="hisD"
                     /locus_tag="VFF98_12440"
     CDS             complement(13043..14374)
                     /gene="hisD"
                     /locus_tag="VFF98_12440"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZU64484.1"
                     /translation="MAIWLKRGAAAEEKAAADRKVRDIVEAALADIEKRGDAAVREMS
                     IKFDGWDREDYRLSQAEIDAAVDSLSPQERKDIEFAQAQVKNFARIQRDSMLDVEVET
                     LPGVILGHKNLPVNAAGCYVPGGKYPLLASAHMSVITAKVAGVPRVITCAPPFQGKPA
                     RAIVAAQALAGADAIYALGGIQAIGAMAIGTQTIDPVDILVGPGNAFVAEAKRQLFGR
                     VGIDLFAGPTETLIIADEIGCDPEMAATDILGQVEHGPDSPGVLLTNSEKFARATIAE
                     IERLLEILPTADHARKAWNTFGEVIVAESYEEMVQIADEIASEHVQVMTADPDYFLKN
                     MTNYGALFLGSRTNVSFGDKVIGTNHTLPTKKSARYTGGLWVGKFLKTCTYQKVLTDE
                     ASALIGEYCSRLCALEGFAGHGEQANIRVRRFGGRNVEYAGQAEPSELTRA"
     gene            14539..15483
                     /locus_tag="VFF98_12445"
     CDS             14539..15483
                     /locus_tag="VFF98_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU64485.1"
                     /translation="MRLATLNNGRPDGQLIVVAPTGTHYAEAPLPTLQAALEDWDAAA
                     PVLAAITDFPHPLDVRQLLAPLPRAWQWLDGSAYPSHGALMDKALGIAPEKPPFPQMY
                     QGVSDTFFAPHADVPMADDALGIDFEGEFGIITDAVPLGTPAAEAGKHIRLLVQINDW
                     SLRKLAGAEMKTGFGWIQAKPPCGMAPFAITPDELGDHWRRARVCLTLKVDWNGANFG
                     NAHGEPMAYGFDELVAHAARTRNLVAGTVIGSGTVSNENCREVGSSCIAERRGIEIVD
                     KGAAETEFMGWGDSVRMEARLPDGSAPFGVLEQKVVKA"
     gene            15480..15998
                     /locus_tag="VFF98_12450"
     CDS             15480..15998
                     /locus_tag="VFF98_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZU64486.1"
                     /translation="MSEDVSDSGLPLVQRVVTGHNEEGRAFFKSEDLSPTRMIPSGDA
                     SFLTIWSTENVPADNNDERDGRDLPTGLTLERGSVIRITDMLPGKESPMHRTNSVDYG
                     IVLKGEIELELEDGRKTTIREGGIIVQRGTNHLWRNTTDKPTRIAFILIEAPAYLHNG
                     QPLAEAKPENAH"
BASE COUNT         2887 a         5524 c         5042 g         2746 t
ORIGIN      
        1 gctcgaaatc gaaatagggc ggcgccggct tgcgatcgga ttcgccaaag cccaccagat
       61 ccatcgcatg gacatgatag cggttggcca gatcctccag caccagccgc cagttgccga
      121 tggtggaggc gcccggcccc gagccatgga tcagcagcag cggcaggccc gccccgccga
      181 tccggcagcc gacggacagc ccctcgaacg accagtggct gagcggcagg cccatggccg
      241 cgctcacagc gccgcgatgg tctgcacctc gacaaggaat ggctccttca cgaggcggtc
      301 gatcatcagc agggtgttgg gcggatagac gccatcgggg aagaactgcg gaaagcggcg
      361 caggcggaaa tccatgaagc ggggaatatc ctgggaatgc accagatagg tggtgaactg
      421 gacgacattg ccccaatcgg ccccggcatc cagcagcgcg gcatggatgt tgcggaacac
      481 ctgctcgcac tgggcatcga aatcgccctc gccgatgatg ttgtcctgcg catcgcccgc
      541 cagctgcccg gcgatgaaca ggaactcatt ggccttcacg cgggtcactt gcgaatattg
      601 gcccatgggc gtgccaagcg tcttgggatt atagatctgg atatcggcca tcttgtcctc
      661 tgggggaagg agtgaggaag gggggaggaa gggaattcag gcggcgcttt tgccgcgctt
      721 gcccacctgc ttgcgcaggc gcagccattc gacgaggcgc ttcatcgtgc ccagcgcatg
      781 ttcgcgggcc acttcgccgg ccagcgtggc attgcgctcg cgcacggcat catacagctg
      841 gcgatgctcg ctcgaggcat cctcgacatg gccggtggca tgggcctcca gccgcacata
      901 gggctctgtc gcatcgcgca ggttgctcac cgtggcggcc agtcgctcgc gcccggcgca
      961 ggccaccagc cggtggtgaa agcggcggtt gagatcgagc cattccggga aattgcccgg
     1021 cgtatgcgcc tcgatctcct ccatctgcac cagcagcgcc tcgatctcgc gcaggtccgc
     1081 ctcggaacgc atgcgcgcgg ccacttccag cgcgtgctgt tccaccacca tgcgcagctc
     1141 gaacagctcg atgatctcga cgacatcgag ttcggccacg gcaaagcccc ggcgcgggcg
     1201 cagctcgatc aggccatcgg cctgcaggcg gctcagcgcc tcgcgcagcg gcacccggct
     1261 gacaccatac tggcgcgcaa gcacttcctg gcgcagctct gcccccggcg cgaaacggcc
     1321 atcgagaatg gcctcgcgca cggcctgata cagcgtttcg ggaagggagg aattgtcggt
     1381 ttcgagcaag gtgggccccg gattggcgtg gtcggaaatt atgtattcag tttttggggt
     1441 tttcgctccg ccgtcaagcc ggatttttcg ctgatcagcg cctgacctgt cgattcgact
     1501 cggacatttc caaaaatatg gcggaatttc agcaaaaata gtgctggaaa attttgtcat
     1561 tgacacagat caacattgta gattgtattc aataatcatc acccctcctg ccacggagtt
     1621 tccccatgac cacgaacgcg gccatccagt ggccggcgga ggattgctcg cgcattcctt
     1681 tcgcgatcta tcacgacccc gccgtctacg agcgcgagca ggaagccatc ttccgcggcc
     1741 ccacctggaa ctacatcggg ctggaagccg aactgccgaa caagggcgat ttcatcacca
     1801 cccgcatcgg cgccacgccg atcctcgtca gccgcgacaa gaccggcgcc gtaaaggcct
     1861 tcatcaaccg ctgcgcccac cgcggcgcgg aactgcggcg cgagccctgc ggcaatgccc
     1921 agctccacgt ctgcgtctac catcagtggt cgttcgagct ggacgggcgg ctgcgcggcg
     1981 tgccctttcc cagcggcgtc aatggcgtgg gcggcctgcc cagggatttc gacaagaagg
     2041 ccatccgcct gcacgagctg accgtgcgct cctacaaggg cgtgctgttc gccacgttcg
     2101 atgccaccgg cgaaagcctg gaagactatc tgggcgaggc catctgtggc gaactggacc
     2161 gcatgttcgc ccggccgatc aagatcctgg gctatcagcg ccagcgcatg aacggcaact
     2221 ggaagcttta taacgacaac gtgcgcgacc ccaatcatgg cggcctgctg cacatgttcc
     2281 acgccacttt cggcctctat cgcctcagcc agatcggcgg cgcgcggatg gaccccaagg
     2341 gccgccacaa catcacctac aaccagctcg acagcgacga tgtggccgcc agcgacgaag
     2401 gctacaagga cacgcagaag gtctattcca agggctacaa gctggaagac atgcgcatgc
     2461 tatcgaaccg cacggaattt cccgatgcgg tgacgctggt gatcgcctcg gtgttcccca
     2521 acagcgtgtt ccagcagatc gccaactcgc tctgcactcg ccagatccgc acctatggcg
     2581 tggacgagat ggagctttac tggacctatt tcggctatac cgacgacagc gaggaaatga
     2641 cccgccaccg cctgctgcaa tcgaacctgt gcgggccggg cggctatatc tcgatggaag
     2701 atggcgaggc ggtagagatc gtccaccgcg ccaccgcccg cgaaaccgat gcccatgcca
     2761 tctgcgagat cggcggcaag ggccccatcg tcgatcagcc caatctggtg agcgaagtgc
     2821 cggtgcgcgg cttctatggc tattacacgg atctgatggg ctttgccaaa ggcccccagg
     2881 cccaggaggc gctggcatga gcgtctcgat ggaacagcgc ctggccgcgc tcgatctcat
     2941 cgcccgcgcc gccaacagcc tcgatgccga ccgcatggag gattgggccg agtgcttctt
     3001 cgaggatggc agctatcaga tcctctcgcg cgagaatgtc gagcgcggcc tgccgctgcc
     3061 gctgttcatg gcggaaaaca agcagatgat ccgcgatcgc gtgctctcgc tgcgcaaggc
     3121 caatgtcacc aacccgcacc gcgatcgcca catcatcggc cctgccgaaa tgcgggcggg
     3181 cgcggatggc gaagtggtga gcgaggcggg ctttgccgtc tattccacca cgcctgaagg
     3241 ggtcagccgg ctgttcagcc tgggtgtcta caaggatcgt attcaaattg gcgatgccgg
     3301 cgcccgcttt gtcgagcgca ccgccatcgt cgacatgttc tccatcccca ccatgctgtc
     3361 caccccggtc tgatccgggg ctggccagcc atctgcgcga gccgggcaaa agacccggcc
     3421 gcatccttgc accaggcggg cgccgttttt tcaacatgag ggacagacga tgcgtatcca
     3481 taaaatcaat cgaacgctgc tgataacggc ctgctccacc ctggcgctga tcgccagccc
     3541 ggcctgcgcg caaacgggcg ctgcacccgc cccaacgaaa gccgacgggc tgggcgagat
     3601 cgtggtgacc gcagagcgcc acgacagcac catccagaaa acccccatcg ccatcgccgc
     3661 ctattccgcc gcccagctgg agcgcgaggg cgtgaccagc ctcgctggcc tcaccgccat
     3721 tgcccccaac ctgggctttg ccaccgtggc cgggcagagc atcgtcacca tccgcggcat
     3781 ttccagccgc gacacatcgg aagtgggcga tccggccgtc gctgtcagca ccgatggctt
     3841 ctacaacaac cgcccctatg ggctgaacgc ctcgctgttc gatctggagc gcgtggaagt
     3901 gctccgcggg ccccagggca cgctcaacgg gcgcaatgcg gtgggcggcg cgatcaacgt
     3961 cgtcaccgcc aagcccacca aacagttcgc cgccgatgtc agcctgggct atggcaatta
     4021 caacgccatc gaggcgcgcg gcatggtcaa cgtgccggtg agcgaccggc tgcagctgcg
     4081 cgccgccatc ctcagcacct cgcatgatgg ctatcgcaac aacgcgccgg ccgccaatgg
     4141 cgatgacgag gacaccaagg ccggccgcct ggccatcgcc ttccagccca ccgatcgctt
     4201 cagcggcctg gtcgagctgg aaatgatcaa gatgggcggc gccggccagt cgctgctcaa
     4261 cgtgcccttc agctataccg gcagcgcggt gaaccacaat gtggccgccc ccaccgccag
     4321 cgcggccggc tgggcctcct acaagtcggg cgtgctcgac atgaaccaga agaccttgcg
     4381 ctggcagttc gattatgccg caggcccggt gaagatcacc tatctgggcg gtttccagag
     4441 cctttactgg cacgccaccg ccgatctctc gctggccggc acgccgcgcc agttcgtgca
     4501 gatcgagcag ccctccaccc agaatcacga attgcgtttt gcctcggcca ccggcgggcg
     4561 cttcacctgg caggcgggcg tgttctattt ccgcgaggat tccagcctgc actcctataa
     4621 cgaatatacc tccgcggccg gggtctatcc gggctttgcc tattattatg gcccgatcgc
     4681 cacgcaatcg gtggccggct atggccaggc cagctacaag ctgaccgatg ccctgaccct
     4741 gaccggcggc ctgcgctaca ccgacgacag caaatcgcgc agcggcggct attacatccc
     4801 caacggcacc accaatccgg tcagctacac cggctacacc ggcaccaaat cgggccactg
     4861 gagcaagctg acctatcacg ccgcgctcga ctggcaggcc agcgcccgca acatgctcta
     4921 tgccaaggtg gataccggct acaaggcggg cggcttcgac gatgtcggca cctatcagcc
     4981 cgaaaccgtt accgccttcg agatcggcgc caagaaccgc ttctggggca acaagctgga
     5041 gctgaacctc tccgccttct acgacgacta cagcaaccag caggtctcgc agctgatcca
     5101 gttcggcgac aatgccggcg ccacccatgt gcagaacgcc ggcaaaagcc acatctacgg
     5161 catcgaggcc gacagcgtgg cgatgctctc gcccgacgac cggctgaacc tgacggtgga
     5221 ctggctgcac gccaaattcg tgcagttcat cggctccacg cccgacagca ccgccaatgt
     5281 gaactttgcc ggcaatgccc tgccgcaatc gccggtgtgg acgatcgcgg ccggctacga
     5341 acatggcttc gatgtggccc atggccggct gaccgcgcgc gccgaaacca agttcaccag
     5401 cagccagtac tacgaatatt acaactacgc cgacatgcgc caaggcgcct acaccaacac
     5461 cacgctgagc ctggcctatg cgcccgccag caagggctgg caggtggaag cctatgttca
     5521 caatctcgag aacaacagca ccctcgccta tgccgaggaa aactacatcg tgaacaccta
     5581 ccgctacgcc ttcaacccgc cgcgcaccta cggcatccgc ttcagcgccc atatcaaata
     5641 gagcccgcct aggccgggcg cgccacatcg aaggggcgcg cccggccctt tttccttgcc
     5701 agacaggacc agacatgacc gacaccccgc ccgcggcccc cgccacccat ctggcccagc
     5761 gcgtggccgc ccttaccgat gccgagaagg cggaactgct gaccggccag ggcatgtggc
     5821 gcagccacgc catcggccgg ctgggcatcc ccgccctgct gatgaccgat ggcgcctatg
     5881 gcgtgcgcta ttccatcagc cagatcgacg gccagcagca gggcgggcag gatctcgacc
     5941 agttcctcgc cgtggtgaac acccgcgcgc gcgacatgaa ggccgctttc ggcaatacga
     6001 agccggccac ctgctatccc aatggctcgg ccattgcctg cagctgggat gtggccaccg
     6061 cctatcagct gggcgccagc ctggccgccg aatgccgcgc ttttgacgtg aacatcctgc
     6121 tggggccggg catcaacatc cgccgcacgc cgctgggcgg gcgcggctat gaatattatg
     6181 ccgaggatcc ggtgctgacc ggcgattttg ccgccgccgt ggtggaaggc ctgcaggatc
     6241 acggggtggg tgcctcgctc aagcattttg cctgcaacaa ttccgagatc gagcgcacca
     6301 ccatggattc gcgcgtgtcg ccccgggccc tgcgcgagat ctatctgctg ggctttgaac
     6361 gggtggtgcg caaggcccgg ccgtggacgg tgatgagctc gtacaaccgc ctgaacggca
     6421 tgcagaccgc gcaggacccc tggctgctga ccgaactgct gcgcggcgaa tggggctttg
     6481 acggcgtcgt cgtctccgac tggcacggca tcaaggaccg gcccgccgcc cttgccgccg
     6541 gcaatgatct cgacatgccg caaaacgcca gccgccagcg cgcgctggca gaagcgatcg
     6601 ccgatggcag cctgccggca gaggtggtga accaggcctg cgcgcgcatg ctggctctgg
     6661 tggacaaggc caaggccgcc gccggcccgg tggtgccctt cgatgccgcc gaacaccacg
     6721 cccacgcccg cgccatggcg gccgcttcca tcgtgctgct gcgcaatgaa gggggcctgc
     6781 tgccgctgca gccggctgcc ctgcgccgca ttctggtcgt gggcgatggc gcggtaaacc
     6841 cggtgatcca gggctcgggc tcggccacca ccctgcccac cgcgcgcgac atcccgctcg
     6901 acgagattgc ccgccaggcc ggcgcggggg tgatcgtgca gcacctgccc ggccatggcg
     6961 acacgcccga ggaaaccgcc gcccgcaccg ccgaggccgc caaagcggca gaaggggccg
     7021 atgccgtcct cgtctttgtc accaccgatg cggccgagga tggcgaaggc gccgaccgcc
     7081 gcacgctggc gctggcccgc ggccatgatg cgctgatcga ggcgctggcg gcggcatcgg
     7141 gcaaggtggt ggtggtgctg gccaaccccg atgcggtggt gatgccctgg gcgggcaagg
     7201 tcgccgccat tgtcgaaacc ttctttgccg ggcaggcgat gggcggcgcc ctggccgata
     7261 tcctgtttgg cgcggtgaac cccagcggca agctcaccgt ctccttcccg gcgcgcatcg
     7321 aggatacgcc cgcctacctc tcctatcccg gcgaggccgg cgtgcatgag tatgccgagg
     7381 ggattttcgt cggctatcgc tattacgaca agcgcgccat cgccccgctg ttccccttcg
     7441 gctttggcct cagctacacc tcctttgcct acagcgatct ggaagtggca acgcccgccc
     7501 ttgccgaggg cgaggatctg cgcattgcct tcaccctggc caacaccggc cctgtggcgg
     7561 gcgcggaagt ggcgcagctc tatctcggct ttcccgaaag ccgggtgcag cgcccgttgc
     7621 gcgagctgaa gggctttgcc aagcgcatgc tggcccccgg cgagaccacc cgcgtggagc
     7681 tggccgtgcc cgcccgcgac ctgtgcttct ttgatgcaga agccggccgc tggcggctgg
     7741 aggaaggcgc cattgccatc gaactgggcg cctcctcgcg cgatatccgg ctggcggggg
     7801 cggcgcgcgc gcagcccgcc cgcccgcatt atcccccgct cgcgatcgac agccagccca
     7861 ccaaggtgct ggccaacccc gtcgcacgcg cgcatttcga gcgcttcctt gccgaaaagc
     7921 tgggcctttc gcccgatcag gccgcgcgca tgctggccta cacgcgctcc tccttcttcg
     7981 gcattcacac cacgctgaac tatttcttcc ggcaggatct gccccgcgcc gagatcgccg
     8041 ccatcatcgc ggccatcaac gccgaaaccg ccgcctgacc gcccatagcg aaggacaccg
     8101 catgaccagc cccctcccgc ccatcgccgc ctatgtctat ggcgagaaga agccggtgcc
     8161 cgaagccttg cgcggcttca tcacccatgc cgacaccgcc gtcgtctcga tcgacatgca
     8221 tctgggccat ctttcggaca aacccgaatg cccctgcccg gcgccgcgcg cgcgcgccat
     8281 catcgatccg gtcaacgcct tccaccgcgc cgcgcgcgat ctgggcattc cggtcatcca
     8341 tgtccgctcg gtgctgcgcc gtaccggcga ggatgatctg aaggggctgg cgccggccgc
     8401 ctggcgttcg ctggtggaac tgcatgggcc ggccatcccc aatatcgccg aacacgccat
     8461 cgagggcagc gaatggacgc aatttgccac cgaagtgctg gacagcgacc ttatcgtcga
     8521 gaccaagcgg cgcctgtcgc ccttctatcc caccgatctg gatttcctgc tgcgcaacat
     8581 gggcgtgcgc cgggtggtgt tcaacggcgg catgactgac tgctgcgtgc tgaacgccac
     8641 cttcgatgcc agcaaccgcg gctatcgcgt ggtggtggcg cgcgatctgg tgcgcggcag
     8701 caatgccgag atggaggacg ccgcgctcaa gatggtctcg ctgcacaccg gcctggtgat
     8761 ggaagccgac gacatcctca cccaatggcg cgccacccgg ccctgatctt ccccacacga
     8821 aaggcctttt catgatcgac atcgtaggcg gcatcagcat gtcgcatgtg ccgggcgcgc
     8881 tgggctggcc ggatgccccc ccggccgacc agcaggcgcg cctggccgcc atccataccc
     8941 acctgcgcga acggctggcg gcggcccggc ccgatctcat cgtcgccttc ctcgacgatc
     9001 atttcgagaa ccatttccgc aacctgctgc caacgctggg ggtgggcatc gcgccggccc
     9061 atatcggccc ggccgcatat atgatggaag ccctgcgctt cgatcgccag catgccatcc
     9121 ccagcgatgc cgatctggcc gaggggctgc tgcgcgagct ggtgcatgcc ggcttcgatg
     9181 ccgcccgcat gggggccatc gaatatggca acaatctgat gatgccgctg cactatatcc
     9241 gcccggaatt cgatattccg gtgctgccgg tgttcatcaa cgtgttctcg ccgccgctga
     9301 tgccctatgg ccgcgcctat gatctggggc tggccacgcg ccgcgcactg gaagcgattg
     9361 gcggcgatcg gcggatcctg ttcctggcca ccggcggcct ctcgcactgg cccccggtgt
     9421 ggaccgaagg cgcgcccgag gacgacagct tcctccagcg gatgaaaacc tatcagaccg
     9481 agggcaaggg cgtgatcgcc gatgatcccg atctctacac cgatctggcc cgctacgaga
     9541 tgaagatgat ggacaagatg cagtggccgc tgggctatca gcatccgctg gtgaacgccg
     9601 agttcgacga ggagttgctg gcccgctttg ccgagggcga tgtgcgctac atgcgcgaga
     9661 tcacctatga cgaggtggag cagcgcggcg gccatggcgg ccatgaattg ttgaactggg
     9721 tggcgctgat gggggcgatg aacggcgccc gggccgacta taccgcctat gagccggtga
     9781 cggaatggat caccggcatg gcctatgccg cctatgacgg gcaacagggc tgaggcaggc
     9841 gcacaaaagg agagggcggc gggggccggc gatacccccg ccgccggttc cctcagatgg
     9901 cgtgttcatc atcctcggtg atcgcggcca gatggccatc caccggctgc ttgccgggct
     9961 tggccttgcg atagggctcg aagctcttgc ggtcggcgtc gaaggccatg tcccagggga
    10021 tatcgggccc gcggaagatc ggcggggcat tgttcgacca cagcagcgaa ccaaagcgga
    10081 aatcccaggc gcgcggcacc caattgtcgt ccagatagtc ggtgtcggcg tggtattccg
    10141 cctcgccgcc cttgccgggg atgtcgcaat aataatagat cgcgctggaa atgcggtggc
    10201 gcgagatgcc gccgctggaa ttcatgctct cgttcttcca gcccttttcg gccatcagat
    10261 tggcgcccag catcacctcg tcgatatcgt ccatgccaaa ggcgatgtgc atgaacttga
    10321 aggcatcggg cgcgaagggc aggtcgcagt tcacccagaa gatcgagtga tgctcgaacg
    10381 tgccgcccgc gcgcgagaag atgcccacgc ccttcgaatg atcgacatag cgaaagccca
    10441 ggtgccgctc ataaaattcg aaagcgccga cataatcggg gctgaagaac accacatggt
    10501 tgatcgtctt ggggatggcc ttatggcgcc agatgcggtg ctggttgaag cgcggatggt
    10561 tgcccggcgt gttgaccggg ctggtctcgc tggccaccgg ccgcttggcc cacacgcgca
    10621 gggcaatcgg ctggccatcc gggcacacgc aatgcaccgt gccatccgca tcgcgggtga
    10681 tcgccacttc gctggcgagg ctggcggcga tgcgctcgag gttctcggcc gtatccacgc
    10741 cccacacggt ttccttcacg ccattgccgg cgaagggatc ggggccgggc aggcgcgcat
    10801 cgccatgggc atagaggatc accctgctgc ccgaggccag gcggaacacc gcctcatcag
    10861 ccgcgatgcg ttccaccggc aggccgaagt cgctccagaa gcgggcatgt tcggccacat
    10921 catcgacgcc gaacaccacg ctttccacgc ccagaatgct catactatct cccgatcctt
    10981 gatctctttg tgcctgcact tatgaccggg ccggcagcgc cccgtaaaac gcaaagagca
    11041 aaaggctggc cataggcgcg gtggatagca gccccgccag cccctcgctc agccccttca
    11101 gctcatcgcg gcgaaggttt gcgcgcacca gtcggcgata tagtcgcgca tgccggtgcc
    11161 attgtcggcg ccgcaatgct ccacctcgaa atcggccttg gtgaacatgc gcaggtgccg
    11221 gttggggctg ttcaccgcct gatcgtagga aagctgggcg ttgaaggcgg ggatctgccg
    11281 gtcgttctcg ccatgggtga tgaggaaggg cacgcggatc ttttccacct gcccgttcag
    11341 cgtgatctgg tcggcatagg caaggaaggc ctcgcggtcg tcatagccga agacccacag
    11401 cacatggtcc cagtagtggg gcacggggtt ctcgccctcg ttcttcacgc gttcgcgctg
    11461 gcgctggccc cacacatggt tggcccccat caccgcgcac agcgcatagc gcggatcgtt
    11521 ggcggcaatg cgcggcgcat gatagccgcc gaaggagagg ccaaagatgc cgatctggct
    11581 gtggatcaca tccgagcggg tcagcagata gtcgaaagcc gggctgcccc agcgctcggc
    11641 atcatacacc gccggcaggc cgcgcttgcg gatcgcctcg ccggtgcccg gctgatcgag
    11701 gaacagcgtg ttcatgccgc gctcgaggtt cgattggccg tggccggcca tgttgacctg
    11761 ttccttcatg gaatcgaggc cattgaccga gaccagcgtg ggccgcggcg tgccgctgcc
    11821 atcatggacg aaaatcgccg tgtaggcgct gccctcgtag gggatctcca ccttgtccac
    11881 atgcaggccg cgcagcgcgc tgcccttgtg gtagaggtcc agccccttgt cataggccac
    11941 ccagcgcggc gcataatcgc ggctctgcat gcgctctgcg gccaggtaat agcccgaggc
    12001 gcgcaggaac ttggtgccgg ccgacaggcg atagcccgcc gccagatcgt cttccgcgtt
    12061 ctgcgccacc tgatcggcaa cggcgatcca gctgtcgaac aacagggcgc tgccggcatc
    12121 ggccccggcc ttggaggcct cgcgcacggg gcggcaggcc tcgtcgatct cgccatggtt
    12181 gccgccgcac accagcgcca gattggtggc caggttccac acgtaattgc cggggaatgg
    12241 ttcaaacatc ggggaaatcc ttaatcggcg gtccagccgc catcgacgat gagggaggtg
    12301 ccggtgatga gggcagaggc gggcgaggcc aggaacagca cgctgcccat cagatcttcc
    12361 acccggccca gccgccccag cttgatcttg gccagcaccg aagcgcggaa ggcctcatcc
    12421 tcgaagaagg gtttggtcat cggcgtttcg atgaaagtgg gggcaatcgt gttggagcgg
    12481 atgccatgcg gcgccagatc cagcgcaaag gccttgttca tcccctctat cgcccatttg
    12541 ctggcgcaat agaggctgcg gcgcggcccg ccgacatggc ccatctggct gcccatatgg
    12601 atgaggctgc cggcgatccc ttcgcggatc atcgcccttg ccgcgccctg ggccacgaag
    12661 aaggcgctct tcacattgag atcgagcacc gcgtcatagt cttcgggcga gacctcccac
    12721 atcggcttgg gccggttggt gccggcattg ttgaccagca cgtggaacgc cggctggccg
    12781 gcgaagaaag cctccacgcc ggccaggtcg ctcacatcca gcaccgctgc ctgcgcactg
    12841 ccgccggccg cgcggatagc ccccgcgccc gcctcgatct cggcggcagt gcgcgcggcc
    12901 agcaccacat gggcgccggc ctctgccaga gcggcggcgc aggcaaggcc aatgccccgc
    12961 cccgccccgg tgaccagcgc gcgctgcccg tccagcctga aaccgggcgt gcggggcagg
    13021 ctctcgctca tgcccccctc gctcatgccc tggtcagttc gctgggttcg gcctggccgg
    13081 catattccac attgcggcca ccaaagcggc gcacgcggat attggcctgc tcgccatggc
    13141 cggcaaagcc ttccagcgcg cacaggcggc tgcaatattc gccgatcagc gccgaggcct
    13201 catccgtcag caccttctgg taggtgcagg tcttgaggaa cttgccgacc cacaggccac
    13261 cggtgtagcg cgccgatttc ttggtgggca gcgtgtggtt ggtgccgatc accttgtcgc
    13321 cgaacgagac attggtgcgg ctgccgagga acagggcgcc ataattggtc atgttcttca
    13381 ggaaatagtc ggggtcggca gtcatcacct gcacgtgttc gctggcgatc tcgtcggcga
    13441 tctgcaccat ctcctcatag ctttccgcca cgatcacctc gccaaaggtg ttccacgcct
    13501 tgcgggcatg gtcggcggtc ggcaggattt cgagcaggcg ctcgatctcg gcgatggtgg
    13561 cgcgggcaaa cttctccgaa ttggtgagca gcacgcccgg gctgtcgggc ccatgctcca
    13621 cctggccgag gatgtcggtg gcggccattt ccggatcgca gccaatctcg tcggcgatga
    13681 tcagcgtctc ggtggggccg gcgaacaggt cgatgcccac gcggccgaac agctggcgct
    13741 tggcttcggc cacaaaggca ttgcccgggc ccacgaggat atccaccgga tcgatggttt
    13801 gcgtgccgat cgccatggcg ccgatcgcct ggatgccgcc cagcgcatag atcgcatcgg
    13861 cgccggccag cgcctgcgct gccacgatgg cgcgcgccgg cttgccctgg aacggcggcg
    13921 cgcaggtgat cacgcgcggc acgcccgcca ccttggcggt gatcacgctc atatgggcgg
    13981 aagccagcag cggatatttg ccgcccggca catagcagcc ggccgcgttc accggcaggt
    14041 tcttgtgccc caggatcacg cccggcagcg tttccacttc cacatccagc atggaatcgc
    14101 gctggatccg ggcgaaattc ttcacctgcg cctgggcaaa ttcaatgtcc ttgcgctctt
    14161 gcgggctcag gctgtccact gccgcgtcga tttcggcctg gctcaggcga taatcctcgc
    14221 ggtcccagcc gtcgaacttg atcgacatct cgcgcacggc ggcatcgccc cgcttctcga
    14281 tatcggccag cgccgcttcc acaatatcgc gtaccttgcg gtctgccgca gctttctctt
    14341 ccgccgccgc accgcgcttc agccaaatgg ccatcgccac aatctcccta tcgaggccct
    14401 gttttgcccg ccttgccacg gccccccaaa cccgaccatc gcatcttatc gatggctgcc
    14461 tatcgatcca aagccgttgt gcgcgcctga ggggcgcgat agcccatgcc tgttagaata
    14521 tgtgggagat gcgcgcagat gcgccttgca accttgaata acggccgccc cgacgggcag
    14581 ctgatcgtcg tcgcgcccac gggcacgcat tatgccgaag cccccctgcc caccttgcag
    14641 gcggcgctgg aagactggga cgcggccgcc cccgtgcttg ccgccatcac cgattttccc
    14701 catccgctcg atgtgcgcca gctgctggcg ccgctgccgc gcgcgtggca gtggctggat
    14761 ggctcggcct atcccagcca tggcgcgctg atggacaagg cgctgggcat cgctccagaa
    14821 aagccgccct tcccacagat gtatcagggc gtgtccgaca cgttctttgc cccccacgcc
    14881 gatgtgccga tggccgatga cgcgctgggc atcgatttcg agggcgagtt cggcattatc
    14941 accgacgccg tgccgctggg cacgccggcg gccgaggcgg gcaagcacat ccgcctgctg
    15001 gtgcagatca acgattggtc cctgcgcaag ctggccggcg ccgagatgaa gaccggcttc
    15061 ggctggatcc aggccaagcc cccctgcggc atggccccct ttgccatcac gccggacgag
    15121 ctgggcgacc actggcgccg cgcccgcgtg tgcctcacgc tgaaggtgga ctggaacggc
    15181 gccaatttcg gcaacgccca tggcgagccg atggcctatg gctttgacga gctggtggcc
    15241 catgccgccc gcacccgcaa tctggtcgcc ggcacggtga tcggctcggg cacggtgtcg
    15301 aacgagaatt gccgcgaggt cggctcctcc tgcatcgccg agcggcgcgg catcgagatc
    15361 gtcgacaagg gcgcggccga gaccgaattc atgggctggg gcgacagcgt gcgcatggaa
    15421 gcccgcctgc ccgatggcag cgcccccttt ggtgttctgg aacagaaggt tgtgaaagca
    15481 tgagcgaaga tgtttccgac agcggcctgc cgctggtgca gcgcgttgtc accggccaca
    15541 acgaagaggg ccgcgccttc ttcaaatcgg aggatctctc gcccacgcgg atgatcccct
    15601 cgggcgatgc cagcttcctg acgatctggt cgaccgagaa cgtgcccgcc gacaacaacg
    15661 acgagcgcga cggccgcgac ctgccgaccg gccttacgct ggagcgcggc agcgtgatcc
    15721 gcatcaccga catgctgccg ggcaaggaaa gcccgatgca ccgcaccaat tcggtggatt
    15781 atggcatcgt gctgaagggc gagatcgagc tggaactgga agacggccgc aagacgacga
    15841 tccgcgaagg cggcatcatc gtgcagcgcg gcaccaacca cctgtggcgc aacacgaccg
    15901 acaagcccac ccgcatcgcc ttcatcctga tcgaagcgcc ggcctatctg cacaatggcc
    15961 aaccgctggc cgaagccaag ccggaaaacg cgcactgaaa acggcacacg cccccgagca
    16021 tgattgttcg ggggcatttt tgatggattt cgactgagca aacgcaagca ccccccctcg
    16081 tcgtaaaacg agggggggtg cttgcgtgta tgtggtgctt gtgatgggtt tttatacgcg
    16141 ggtagcaatg cctggcgacg ccctactctt ccaacgcttg agcgttagta ccattggcg
//
LOCUS       DASRYE010000243         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_184861,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000243 DASRYE010000000
VERSION     DASRYE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184861"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFF98_12455"
     CDS             <1..681
                     /locus_tag="VFF98_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64487.1"
                     /translation="QAVPPMNYGDGQYGGQQDAPPPGGGDYRNPVPPADQAPQGYDAP
                     PSEPGAPEGYDPSRPPPPPQGYAAPEDMAAINAADQRYAYEAQRWAAENCVKSHGDTA
                     AGAVVGGIFGAILGGAIGGGHGGAVAAGAALGAVGGAAVASNSGGATSPGCPPHYVLR
                     RDAAPYGYAPSGYYYAAPSWYVPWVFVGGAWTYRPYPYHDWYWRTYRAPHGWHGGPGW
                     RGGEHWRR"
     gene            813..1157
                     /locus_tag="VFF98_12460"
     CDS             813..1157
                     /locus_tag="VFF98_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64488.1"
                     /translation="MFFLALGVVLFGTLAPHPPHVPMDSMGDKFEHFTAFAGLALFAR
                     LGFRKMPNWLLLERLSFFGALIEVAQAAPAIHRDCDWHDWVADTMGAWVMLVAIILVR
                     FWLNVRRPFGTV"
     gene            1284..2690
                     /locus_tag="VFF98_12465"
     CDS             1284..2690
                     /locus_tag="VFF98_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU64489.1"
                     /translation="MSTPFRLLIDGALVEGASLLPVVNPALGTPFADAPRADAALLEQ
                     AVAAANRAFPAWAARPWAERAAIVGALGDAVEARADDFARLLTQEQGKPLAEAQSEVA
                     GTVHGFRWYAQQTLPPKVLRENERERIVEHHVPLGVVAAITPWNFPLLLLMLKLAPAL
                     VTGNVVIGKPAPTTPLTTLLLGEVAADLVPAGVFQTLVDGNDLGGALSSHPGVRHVSF
                     TGSTATGKKVLASTADTLKRFTLELGGNDAALVLDDADVARIAPSIFAAATVNAGQVC
                     LATKRVYAPRALYDALCEALGEQARAAAVGDGLEQGTRIGPVQNAAQYEKLKGFLEEA
                     RAEGQIVAGGEVLDRPGYFIAPTVVRDLPDDARLVREEQFGPLLPVLAYDDLEAAIAA
                     VNSTEYGLGGTVWSGDAARGEAVAARIDSGTVWVNRFLDLPFDIPFGGAKQSGIGRQQ
                     GLEGMAEFTQVKVINAAL"
BASE COUNT          385 a          925 c         1020 g          469 t
ORIGIN      
        1 caagcggtgc cgccgatgaa ttatggcgat ggccagtatg gcggccagca ggatgcgccg
       61 ccgccgggtg gtggcgatta tcgcaacccg gtgccgccgg cagaccaggc gccccaaggc
      121 tatgacgcgc ccccgtccga gccgggcgcg ccagagggct atgatcccag ccgcccgccg
      181 ccgccgccgc agggctatgc cgcgcccgag gacatggccg cgatcaacgc tgccgatcag
      241 cgctatgcct atgaggcgca gcgctgggcc gctgaaaact gcgtgaagtc gcatggcgat
      301 acggcggccg gcgcggtggt gggcggcatc ttcggtgcca tcctgggcgg cgccatcggt
      361 ggcggccatg gcggcgcggt ggccgcgggc gcggcgctgg gcgcggtggg cggcgcggcg
      421 gtggccagca attctggcgg cgccaccagc cccggctgcc ctccgcatta tgtgctgcgg
      481 cgcgatgcgg cgccttatgg ctatgcgccc tcgggctatt actatgcggc gcccagctgg
      541 tatgtgccct gggtgtttgt cggcggcgcc tggacctatc gcccctatcc ctatcacgac
      601 tggtactggc gcacctatcg cgcgccgcat ggctggcatg gcggcccggg ctggcgcggc
      661 ggcgaacact ggcgccgctg agccgccgca aggggcgcac gcaaagaagg ggctgggcgt
      721 tcaagaaggg gctggacagg gccgagaatt ctgccacgac caaagcccct tccttgcggt
      781 gagccatgac ctttaccaaa ccttcgcgcc tgttgttttt tcttgctctt ggcgtggttc
      841 tgttcggcac gctggcgccc catccaccgc atgtgccgat ggacagcatg ggcgacaagt
      901 tcgagcattt caccgctttt gcagggctgg cgctgtttgc ccggctgggc tttcgcaaga
      961 tgcccaactg gctgctgctg gagcgcctgt cgttcttcgg cgcgctgatc gaggtggcgc
     1021 aggccgcccc tgccatccac cgcgactgcg actggcacga ctgggtggcc gatacgatgg
     1081 gcgcttgggt gatgctggtg gcgatcatcc tcgtgcgctt ctggctgaac gtgcgccggc
     1141 ctttcggcac cgtctgatcc acagccggca cagctgcgca aggggggccc tatccacttg
     1201 gatagcgccc ccttgcgcag ctgtgcctgc gccccaatat gcccggcggc cgcccctcgc
     1261 gcgccaatcc ggaggagatc ctgatgagca ccccctttcg tctgttgatc gatggcgcgc
     1321 tggtggaagg cgccagcctg ctgccggtgg tgaatccggc gctgggcacc ccctttgccg
     1381 atgccccccg cgccgatgcc gccctgctgg aacaggccgt ggccgcggcc aaccgcgcct
     1441 tccccgcctg ggcggcgcgc ccctgggccg agcgggcggc gatcgtgggc gccctgggcg
     1501 atgcggtgga agcgcgggcc gatgattttg cccgcctgtt gacgcaggaa cagggcaagc
     1561 ccctggccga ggcgcaatcg gaagtggccg gcaccgtgca tggcttccgc tggtatgccc
     1621 agcagaccct gccgcccaag gtgctgcgcg agaacgagcg cgaacgcatt gtggagcacc
     1681 acgtgccgct gggcgtggtg gcggcgatca cgccgtggaa cttcccactg ctgctgctga
     1741 tgctgaagct ggcgccggcg ctggtcaccg gcaatgtggt gatcggcaag cctgcgccga
     1801 ccacgccgct caccaccctg ctgctgggcg aggtggccgc cgatctggtg cccgccggcg
     1861 tgttccagac gctggtggat ggcaatgatc tgggcggcgc gctgtcgtcg catccggggg
     1921 tgcgccatgt cagcttcacc ggttccaccg ccaccggcaa gaaggtgctg gcgagcacgg
     1981 ccgacacgct gaagcgcttc acgctggagc tgggcggcaa tgatgccgcg ctggtgctgg
     2041 atgatgccga tgtggcgcgg atcgccccct cgatctttgc cgccgccacg gtgaatgccg
     2101 ggcaggtgtg cctggccacc aagcgcgtct atgcgccgcg cgcgctgtat gacgcgctgt
     2161 gcgaggcgct gggcgaacag gcccgcgcgg ctgccgtggg tgatgggctg gagcagggca
     2221 cgcgcattgg cccggtgcag aatgccgccc aatacgagaa gctgaagggc tttctggaag
     2281 aggcgcgcgc cgaggggcag attgtggccg gcggcgaggt gctggaccgg ccgggctatt
     2341 tcatcgcccc cacggtggtg cgcgatctgc cggacgatgc gcggctggtg cgtgaggagc
     2401 agtttggccc gctgctgccg gtgctggcct atgacgacct ggaggcggcc atcgccgcgg
     2461 tgaacagcac cgaatatggc ctgggcggca cggtatggtc gggcgatgcg gcgcgcggcg
     2521 aggccgtggc ggcgcgcatc gattcgggca cggtgtgggt caaccgcttc ctcgatctgc
     2581 ctttcgacat ccccttcggc ggcgccaagc aatcgggcat cggccgccag caggggctgg
     2641 agggcatggc cgaattcacc caggtgaagg tgatcaacgc ggccctgtga taatggtgtg
     2701 aatgatggcc ggttcccgct gtgcggggac cggccggcgg caccagccca cgcccccacc
     2761 cggcctcccc tagggtacta tcgttgggag gccgggtgg
//
LOCUS       DASRYE010000244         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_184863,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000244 DASRYE010000000
VERSION     DASRYE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184863"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFF98_12470"
     CDS             <1..834
                     /locus_tag="VFF98_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HZU64490.1"
                     /translation="PPENETAAGHAAPPAPTLPILPILPTLPALALLMAAPFGPPDAG
                     ALEQALAQADPAGRWRARAVDGALRLEGAGGALLLGASGGAELPARVAARSLWLQPGE
                     AAGIAEAQRASLWLRALPGAQADSTGRRRLALAMAVLVAQLVAVRPVLAVLNADRGLL
                     FPARLVPALLAPLAAGEVPLQLFVAIACHGVRAGAISLASEGLQALVGHELEVCEAPG
                     SLAEVAALVEQVARYLLAAGPVIGAGDTLAPAGSGAPWRAAWQDRAVLRLLPPPGVQQ
                     R"
     regulatory      876..1014
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1101..1502
                     /locus_tag="VFF98_12475"
     CDS             1101..1502
                     /locus_tag="VFF98_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64491.1"
                     /translation="MPDSLDSTAWSSLLLGLAALAAGGGALRKPFVWRSMISEVEQSP
                     ALQFLSGMLELVVGAAVYLANGWLPADLLACLLKGLGGLMMIEALAILSMCDLYTQLW
                     LRNLAHFHRGWAGATALLGAVLAGLGMFRFS"
     gene            1530..>3006
                     /gene="thiC"
                     /locus_tag="VFF98_12480"
     CDS             1530..>3006
                     /gene="thiC"
                     /locus_tag="VFF98_12480"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZU64492.1"
                     /translation="MADIASRLDIGVTTGPIRGSAKIHVGPLKVAMREIALEPGSGEA
                     PVRVYDTSGPYTDPAATIDIAAGLAPLRREWILARGDVEAYDARAVKPEDNGQLGPDR
                     SGGVPPFPQVVRQPLRARAGANVSQMHYARRGIITPEMEYVAVRENLGRAALKEQLAR
                     DGQDWGAAIPDFVTGEFVREEVARGRAIIPNNINHPESEPMAIGRNFLVKINANIGNS
                     AVASDVASEVDKMVWAIRHGADTVMDLSTGRNIHDTREWIIRNSPVPIGTVPIYQALE
                     KVGGIAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLPYIPLTAKRVTGIVSRGGSIM
                     AKWCLAHHKESFLYEHFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLG
                     ELTKRAWAQDVQVMIEGPGHVPMHKIKENMDKQLAACGEAPFYTLGPLVTDIAPGYDH
                     ITSGIGAAMIGWFGTAMLCYVTPKEHLGLPDREDVKAGVIAY"
BASE COUNT          449 a          953 c         1114 g          490 t
ORIGIN      
        1 ccgcccgaaa acgagacggc tgccggccat gctgccccgc cggcgccgac attgccgata
       61 ttgccgatat tgccgacatt gccggccctg gcgctgctga tggcggcgcc tttcggcccg
      121 cccgatgccg gggcgctgga acaggcgctg gcgcaggccg atcctgccgg gcgctggcgg
      181 gcgcgggcgg tggatggggc gctgcggctg gaaggggcgg gcggggccct cctgctgggg
      241 gcctctggcg gggcggagct gccggcccgg gtggccgcgc gcagcctgtg gctgcaaccg
      301 ggcgaggcgg ccgggattgc cgaggcgcag cgcgccagcc tgtggctgcg ggcgttgccg
      361 ggcgcgcagg cggatagcac cggccggcgc cgcttggccc tggcgatggc ggtgctggtg
      421 gcgcagctgg tggcggtgcg gccggtgctg gcggtgctca atgccgatcg cgggctgctg
      481 tttccggcgc ggctggtgcc ggcgctgctg gcgccgctgg cggcgggcga agtgccgctg
      541 cagctgtttg tcgccatcgc ctgtcacggc gtgcgggccg gggcgatctc gctggccagc
      601 gaggggctgc aggcgctggt gggccatgag ctggaagtgt gcgaggcgcc cggcagcctg
      661 gccgaagtgg cagcgctggt ggagcaggtg gcgcgctatc tgctggcggc aggcccggtg
      721 atcggcgcgg gcgacacgct ggcgcctgcc ggctccggcg cgccctggcg tgcggcatgg
      781 caggatcggg cggtgctgcg gctgctgccc ccgcccggcg tgcaacagcg ctgacaggag
      841 gggctggaca ggggcggcgc ttgcgcttac cacagggcca ttccccgggg agcctgcatg
      901 ggctgagagg tgggttttac cgcgcggtgc cttgcgttgc cgctgcgggg caccccacga
      961 cccgccgaac ctgaacccgt taatacgggc ggagggaggg agcgcgacgg cctgcctggt
     1021 ggcgtttgcc tgttgcgtgt ctgttcttct cgcccggcct gtgaacccga agaggtgata
     1081 acaaagccga ggagagacct atgcctgaca gccttgattc cactgcctgg agcagccttc
     1141 tgctgggatt ggccgcattg gccgccggcg ggggcgcgct gcgcaaacct ttcgtatggc
     1201 gctcgatgat cagcgaggtg gagcaatcgc ccgccttgca gttcctcagt ggcatgctgg
     1261 agctggtggt gggggcggct gtctatctgg ccaatggctg gctgccggcc gatctgttgg
     1321 cctgcctgct gaaagggctg ggcgggctga tgatgatcga ggcactggcg atcctctcga
     1381 tgtgcgatct ctatacgcag ctgtggctgc gcaatctggc ccatttccac cgcggctggg
     1441 cgggcgccac ggcgctgctg ggcgcggtgt tggcggggct ggggatgttt cgtttttcgt
     1501 gatttttgcg caaggaaaga gtgtagttca tggctgacat cgcatcgcgc ctcgacatcg
     1561 gcgtgaccac cgggcccatt cgcggctctg ccaagatcca tgtgggcccg ctgaaagtgg
     1621 cgatgcgcga gatcgcgctg gagccgggca gcggcgaggc gccggtgcgt gtctacgaca
     1681 cctccggccc ctacaccgat ccggccgcca ccatcgacat cgccgccggc cttgcccccc
     1741 tgcggcgcga gtggatcctg gcgcgcggcg atgtcgaagc ctatgacgcg cgggcggtga
     1801 agccggaaga caacggccag ctgggccccg atcgcagcgg cggcgtgccg cctttcccgc
     1861 aggtggtgcg ccagcccctg cgggccaggg cgggcgccaa tgtcagccag atgcattatg
     1921 cccgccgcgg catcatcacg cccgagatgg aatatgtggc ggtgcgcgag aacctgggcc
     1981 gcgccgcgct gaaggagcag ctggcgcgcg acgggcagga ttggggggcg gccatccccg
     2041 atttcgtcac tggcgaattc gtgcgcgagg aagtggcgcg cggccgcgcc atcatcccca
     2101 acaacatcaa ccacccggaa agcgagccga tggcgatcgg ccgcaacttc ctggtgaaga
     2161 tcaacgccaa tatcggcaat tccgccgtgg ccagcgatgt ggcgagcgaa gtggacaaga
     2221 tggtctgggc catccgccat ggcgccgaca ccgtcatgga cctgtccacc ggccgcaaca
     2281 tccacgacac gcgcgaatgg atcatccgca acagcccggt gccgatcggc acggtgccga
     2341 tctatcaggc gctggaaaag gtgggcggca ttgccgagga cctgacctgg gagatcttcc
     2401 gcgacacgct gatcgagcag gccgagcagg gtgtggatta tttcaccatc cacgccggcg
     2461 tgcgcctgcc ctatatcccg ctgacggcca agcgcgtgac gggcatcgtc agccgcgggg
     2521 gcagcatcat ggccaagtgg tgcctggcgc atcacaagga aagcttcctc tacgagcact
     2581 tcgacgagat caccgagatc atgaaggcgt atgacatcgc ctattcgctg ggcgatggcc
     2641 tgcgcccggg cagcattgcc gatgccaatg acgaggcgca attcgccgaa ctctacacgc
     2701 tgggcgagct gaccaagcgc gcctgggcgc aggatgtgca ggtgatgatc gagggccccg
     2761 gccatgtgcc gatgcacaag atcaaggaga acatggacaa gcagctggcc gcttgcggcg
     2821 aggccccgtt ctacacgctg gggcccctcg tcaccgacat tgcgccgggg tatgaccaca
     2881 tcaccagcgg cattggcgcg gcgatgatcg gctggttcgg cacggcgatg ctgtgctacg
     2941 tgactccgaa ggagcatctt ggcctccccg accgcgagga cgtgaaagcc ggcgtcatcg
     3001 cctatc
//
LOCUS       DASRYE010000245         7334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_184891,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000245 DASRYE010000000
VERSION     DASRYE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184891"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFF98_12485"
     CDS             <1..209
                     /locus_tag="VFF98_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZU64493.1"
                     /translation="DARQQLAERRLVNRSTDTPFGKITLSGGIADVFAFADPRAALRA
                     ADKALYRAKAEGRNRMLIAERETA"
     gene            complement(301..2607)
                     /gene="pnp"
                     /locus_tag="VFF98_12490"
     CDS             complement(301..2607)
                     /gene="pnp"
                     /locus_tag="VFF98_12490"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZU64494.1"
                     /translation="MFDVKTVSLEWGGKTLTLETGRIARQADGAVLATYGETVVLCAV
                     TAAKSVKEGQDFFPLTVHYQEKFSAAGRIPGGFFKRERGATEKETLVSRLIDRPVRPL
                     FPEGFYNEINVIAQVLSYDGETEPDIVAMIAASAALTISGVPFMGPIGAARVGFKDGE
                     YQLNPSMAQVAEGRLDLVVAATGNAVMMVESEAKELSEDEMLGAVNFAHDEARKVINA
                     IIDLAEKAAKDPWEIDLSDNTAEMKAKLKKLVGKDIAKAYKLTDKSARSNALNEVRAK
                     AKAAFAEESPQTQMVAIKVTKKLEAEIVRTAILKDGQRIDGRTTTQIRPIESMVGFLP
                     RTHGSALFTRGETQAICTTTLGTKDAEQMIDGLDGLSYQNFMLHYNFPPYSVGEVGRF
                     GAPGRREVGHGKLAWRALHPVLPAKEDFPYTIRVLSDITESNGSSSMATVCGGSLSMM
                     DAGVPLVRPVSGIAMGLILEGDEFTVLSDILGDEDHLGDMDFKVAGTSEGITTMQMDI
                     KVAGITPAIMKQALEQAKEGRAHILGEMLKSLGEARTELSAHAPRIETINIDKSKIRD
                     VIGTGGKVIREIVATTGAKVDIDDEGIIKISSSDLSQIEAAKVWILGIVEEAEVGKIY
                     KGKVVNIVDFGAFVNFMGGKDGLVHVSEMKNERVEKPTDVVKEGQEVFVKVLEIDQRG
                     KVRLSMRVVDQETGAELEDTRPPREPKADRGDRGDRGDRGGDRRGPRSDRGDRGPRRE
                     GRGDRGPRREREEGGNPDHMPAFLKGDE"
     gene            complement(2840..3109)
                     /gene="rpsO"
                     /locus_tag="VFF98_12495"
     CDS             complement(2840..3109)
                     /gene="rpsO"
                     /locus_tag="VFF98_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZU64495.1"
                     /translation="MSITAEKKQEVIQDNARAANDTGSPEVQVAILTSRIVTLTEHFK
                     GHHKDNHSRRGLLKMVNKRRSLLDYLKKKDVERYNALIAKLGLRK"
     gene            complement(3131..4066)
                     /gene="truB"
                     /locus_tag="VFF98_12500"
     CDS             complement(3131..4066)
                     /gene="truB"
                     /locus_tag="VFF98_12500"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZU64496.1"
                     /translation="MDNAPPKTAPQKPAHGWIILDKPVGLGSTQAVAAVKRNFRQAGF
                     GKVKVGHGGTLDPLASGVLPIALGEATKLTGRLLDADKIYAFTIAFGSQTDTLDLEGA
                     VVASSDVRPTRAALEAALAAFTGPISQVPPAYSALMVEGRRAYDLARAGEAVELPARA
                     VTIHALAVMATEGPEEALASATLRAHVSKGTYIRSLARDIALALGSCGHVTWLHREKA
                     GPFRLEQAISLDKLNEIGQGRPLGTSMLPLEAGLVDIPALSLDPQAVAAVRQGRVLCG
                     MAQADGLYWALDGDVPVALVRLEGGSMKVERGFNL"
     gene            4172..5044
                     /locus_tag="VFF98_12505"
     CDS             4172..5044
                     /locus_tag="VFF98_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64497.1"
                     /translation="MQLLVHIGLHKTGSTYLQYLLNGNHAALARAGFWYAPQPGYPAH
                     HHAAWRLLAGDGAPLAAMIADARQAGCHTLILSSEDLEGALHDPRPLAAIAAAAREGG
                     VDAVAWHVVLREAGAVFASLFAQLQHHIYADSLQMFHDCLRRGHLFYDQPMPGEGTPY
                     WYYSFDVARDLDRLAERLAACGHRLVAHDFADASPFPGWRLLPDAARAVLQALPDAGA
                     RNARLPAEEVAGGYIARIFDIVPDEAGRQAILNAFTTSLSASLEGVAAYAPLVGQRFA
                     ASRQRALATHGAAA"
     gene            complement(5054..5632)
                     /locus_tag="VFF98_12510"
     CDS             complement(5054..5632)
                     /locus_tag="VFF98_12510"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZU64498.1"
                     /translation="MAKLYFYYASMNAGKSTNLLQADFNYRERGMATMLWTAALDDRG
                     RQGAIESRIGLAADARCFDADTDLFAEGIAEQARGPLACVLVDEAQFLDADQVWQLAR
                     LADEAGIAVLCYGLRTDFQGRLFSGSAALLGIADTLVELKGVCHCGAKATMNLRVDAQ
                     GRAVIAGAQTEIGGNERYVALCRRHFVAARGG"
     gene            complement(5639..6046)
                     /gene="rbfA"
                     /locus_tag="VFF98_12515"
     CDS             complement(5639..6046)
                     /gene="rbfA"
                     /locus_tag="VFF98_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZU64499.1"
                     /translation="MARSPATPETRSVRLLKVGEQVRHVISELLARQQVHDDVLTAHA
                     VSVTEVRMSPDLRHAAVFVKPLLGEDEEAVLKALRTNTAFFQKEVAGRLKLKYAARIK
                     FLPDESFDEASRIDALLADPRVRRDVEGEDEPG"
     gene            6307..6714
                     /locus_tag="VFF98_12520"
     CDS             6307..6714
                     /locus_tag="VFF98_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZU64500.1"
                     /translation="MRKILNTLLAAALVAPMLAPAAAMADPYDHGGPGWHGDRDHGPG
                     PGGPGWHGDRGPGWHGDRGWHDGRPGDWRQFRRGERFDRDRAWRYGELDYRAYHGLRR
                     PPRGYHWVRNGDDALLIGITSGIVASVIVGAIR"
     gene            6886..7278
                     /locus_tag="VFF98_12525"
     CDS             6886..7278
                     /locus_tag="VFF98_12525"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZU64501.1"
                     /translation="MKRILSAAIAVAMLAPAVPALAQPGGGPDRGGEHGGDRGGDHGG
                     DHGGGERGGDHGRGGPGGDHGGAPQFRRGEHFDRGRAVNYGELDYRQFHGMRRPPRGY
                     HWVRNGDDALLVGITSGIIASVIVGATR"
BASE COUNT         1156 a         2607 c         2285 g         1286 t
ORIGIN      
        1 aagacgcccg ccagcaactg gccgaacgcc gcctcgtcaa ccgctccacc gacacgccct
       61 tcggcaagat caccctgtcg ggcgggatcg ccgatgtgtt tgcctttgcc gatccgcgcg
      121 cggccctgcg cgcggccgac aaggcgctgt atcgcgccaa ggcggaaggg cgcaaccgca
      181 tgctgatcgc cgagcgcgaa accgcctgag ccccaaaaca aagggcgcgg gaaaggatcg
      241 ctcctctccc gcgccctgtc agcgccagcg gcggggggcc ttgcccccca gcgccaatgc
      301 ttattcgtcg cccttcagga aggcgggcat gtggtcgggg ttgccccctt cctcacgctc
      361 gcggcggggg ccacgatcgc cccggccttc gcggcgcggg ccgcgatcgc cccggtcgct
      421 gcgcggccca cggcggtcgc cgccgcggtc accccggtcg ccacggtcac cgcggtcggc
      481 cttgggctcg cgcggggggc gggtatcttc cagctcagcg ccggtttcct ggtcgacgac
      541 ccgcatcgac aggcgcacct tgccgcgctg gtcgatctcc agcaccttga cgaagacttc
      601 ctggccttcc ttcaccacgt cggtgggctt ttccacgcgc tcgttcttca tttccgacac
      661 gtggacgaga ccgtccttgc cgcccatgaa gttcacgaaa gcgccgaaat cgacgatgtt
      721 gacgaccttg cccttgtaga tcttgccgac ctcggcctct tccacaatgc ccaggatcca
      781 caccttggcg gcctcgatct ggctcaggtc cgacgaggag atcttgatga tgccctcatc
      841 gtcgatatcc accttggcgc cggtggtggc cacgatctcg cggatcacct tgccgccggt
      901 gccgatcacg tcgcggatct tcgacttgtc gatgttgatc gtctcgatgc gcggcgcatg
      961 cgccgacagc tcggtgcggg cctcgcccag cgacttcagc atctcgccca ggatatgcgc
     1021 gcggccttcc ttggcctgct ccagcgcctg cttcatgatg gcgggcgtga tgccggccac
     1081 cttgatgtcc atctgcatcg tggtgatgcc ttcagacgtg ccggccacct tgaagtccat
     1141 gtcgcccagg tgatcctcgt cacccagaat gtcggacagc acggtgaact cgtcgccttc
     1201 caggatcagg cccatggcga tgcccgacac cgggcgcacc agcggcacgc cggcatccat
     1261 catcgacagg gaaccgccgc agacggtggc catcgaggac gaaccgttcg actcggtgat
     1321 gtcggacagc acgcggatcg tgtaggggaa gtcttccttg gcgggcagca ccgggtgcag
     1381 cgcccgccag gccagcttgc catggcccac ttcgcgccga ccgggcgcgc caaagcggcc
     1441 cacttcaccc accgaatagg gggggaagtt gtagtgcagc atgaagttct gatacgacag
     1501 gccatcaagg ccatcgatca tctgctcggc gtccttggtg cccagcgtgg tggtgcagat
     1561 ggcctgcgtt tccccgcggg tgaacagcgc cgaaccatgg gtgcgcggca ggaagccgac
     1621 catcgattcg atcgggcgga tctgggtggt ggtgcgcccg tcgatgcgct ggccgtcctt
     1681 caggatggcg gtgcgcacga tctcggcttc cagcttcttg gtcaccttga tggcaaccat
     1741 ctgggtctgc gggctttcct cggcgaaagc ggccttggcc ttggcacgca cctcgttcag
     1801 cgcgttggaa cgggccgact tgtcggtcag tttgtaggcc ttggcgatat ccttgccgac
     1861 cagcttcttc agcttggcct tcatctccgc ggtgttgtcg ctgaggtcga tttcccacgg
     1921 atccttcgcg gccttctcgg ccaggtcgat gatcgcgttg ataaccttgc gggcctcgtc
     1981 atgagcgaag ttgaccgcac ccagcatctc gtcctcggaa agctccttgg cttccgattc
     2041 caccatcatc acggcattgc cggtggcagc gacaacgagg tcgaggcggc cttcggccac
     2101 ctgcgccatg ctggggttca gctggtattc gccatccttg aagcccacgc gcgcggcgcc
     2161 aatggggccc atgaaaggca cgcccgagat ggtgagcgca gccgaagcag cgatcatcgc
     2221 cacgatatcg ggctcggtct cgccgtcata gctcagcacc tgagcgataa cgttgatctc
     2281 gttgtagaaa ccttcgggga acagcgggcg caccgggcgg tcgatcaggc gggaaaccag
     2341 cgtttccttt tccgtcgcgc cgcgctcgcg cttgaagaag ccaccgggaa tgcggccggc
     2401 agccgagaac ttttcctgat agtggacggt gagcgggaag aagtcctggc cttccttcac
     2461 gctcttggcg gcggtcacgg cgcacagcac cacggtctcg ccataggttg ccagcacggc
     2521 accatcggcc tggcgggcaa tgcggccggt ttcgagagtg agggtctttc cgccccactc
     2581 cagcgatacg gtcttcacgt cgaacatttc gtttccttca tccccacagg ccctattgcc
     2641 ccaggggttg cttatgccgg gattgccgcc cggcggcggt gcggggcttc aggtcttggc
     2701 cccaggcccc ccgccggatt gcgggaaaac ctgtattcct gtgctgccgc cagacatgac
     2761 aagcgccccg gaggcggggc gggccgtgcg ggcagctgat acaagaagcg ccccgctggg
     2821 gggcgcttcc ttctgaacct tacttgcgca ggcccagctt ggcgatgaga gcgttgtagc
     2881 gctccacatc cttcttcttc agatagtcga gcaggctgcg acgcttgttg accatcttga
     2941 gcagaccacg gcgggaatgg ttatccttgt ggtggccctt gaagtgctcg gtcagcgtca
     3001 cgatgcggct ggtcaggatc gccacctgca cctcgggcga accggtatcg ttcgcggcgc
     3061 gggcgttgtc ctgaataact tcctgcttct tttcagcggt aatcgacata tccaaactcc
     3121 gcgacatcga ttagaggttg aagccccgct cgaccttcat cgagccgcct tccagcctga
     3181 ccagcgccac cggcacatcg ccgtccagcg cccagtacag cccgtcggcc tgggccatcc
     3241 cgcacagaac gcggccctga cggaccgctg ccaccgcttg cggatcgagg gagagagccg
     3301 ggatgtcgac cagccctgcc tccagcggca gcatggaagt tccaagcggc cggccctgtc
     3361 cgatttcgtt caacttgtcc agtgaaatcg cctgttccag ccggaacggg ccggctttct
     3421 cgcggtgcag ccaggtcaca tggccgcagc tgcccagcgc cagcgcaata tcgcgcgcca
     3481 ggctgcggat ataggtgccc ttggagacat gggcgcgcaa ggtggcgctg gccagcgctt
     3541 cctccggccc ttccgtggcc atcaccgcca gcgcgtggat cgtcaccgcg cgggcgggca
     3601 gttccaccgc ctcgccggcg cgggccaggt cataggcgcg ccggccctcc accatcagcg
     3661 ccgaataggc cggcggcact tgcgagatgg gcccggtgaa tgccgccagc gccgcctcca
     3721 gcgccgcgcg ggtgggccgc acatcgctgc tggccaccac cgccccttcc agatccagcg
     3781 tatcggtctg gctgccaaag gcgatggtga aggcatagat cttgtcggca tcgagcaggc
     3841 ggccggtcag cttggtcgcc tcgccaaggg caatgggcag cacgcccgag gccagcggat
     3901 ccagcgtgcc gccatggccc accttcacct tgccaaagcc ggcctggcgg aaattgcgct
     3961 tcactgcggc caccgcctgc gtgctgccaa ggcccacggg cttgtcgagg atgatccagc
     4021 catgggccgg tttctggggg gccgtttttg ggggcgcgtt atccatgggc gcgggccata
     4081 gcggcggatg ggggggaaga aaaggcgccc cgcaaaactg cgcaagcctg ccttgccccc
     4141 ctctttacca tccccgcccc acaagggcgc catgcagctt ctcgtccaca tcggccttca
     4201 caagaccggc tcgacctatc ttcaatatct gctgaacggc aaccacgccg cgctggcccg
     4261 ggccggcttc tggtatgcgc cgcagccggg ctatccggcc catcaccatg ccgcctggcg
     4321 cctactggcc ggcgatggcg cgccgctggc agccatgatc gccgatgccc ggcaggccgg
     4381 ctgccacacc ctcatcctct cctccgaaga cctcgaaggc gcgctgcatg atccgcgccc
     4441 gctggccgcc atcgccgccg ccgcgcgcga ggggggcgtg gacgcagtgg cctggcatgt
     4501 ggtgctgcgt gaggcgggcg cggtgtttgc cagcctgttc gcccagctgc agcaccacat
     4561 ctatgccgat tcgctgcaga tgttccacga ttgcctgcgc cgcggccacc tcttctacga
     4621 tcagccgatg cccggcgaag gcacgcccta ctggtattac agcttcgatg tcgcccgcga
     4681 tctcgaccgg ctggccgagc gcctggccgc ctgtggccac cggctggtgg cgcatgattt
     4741 tgccgatgcc agccccttcc ccggctggcg cctgctgccc gatgccgctc gcgccgtgct
     4801 gcaggccctg cccgatgccg gcgcccgcaa cgcccgcctg ccggccgagg aagtggccgg
     4861 cggctatatc gcccggatct tcgacatcgt gcccgatgag gccggccggc aagcgatcct
     4921 gaacgccttc accacctcgc tctcggccag cctggaaggg gttgccgcct atgccccgct
     4981 ggtgggccag cgctttgccg caagccggca gcgcgcgctg gccacccatg gcgccgccgc
     5041 atagccacgg cgcctaaccg ccgcgcgccg ccacaaaatg ccgccggcac agcgccacat
     5101 agcgctcgtt gccgccaatc tcggtctgcg cgccagcgat cacggcgcgg ccctgcgcat
     5161 ccaccctcag gttcatggta gccttggcgc cgcagtggca cacgcccttc agctccacca
     5221 gcgtatcggc aatgcccagc aacgccgccg atccggaaaa cagccgcccc tgaaaatccg
     5281 tgcgcaagcc atagcacagc accgcaatgc ccgcctcatc ggccaggcgc gccagctgcc
     5341 acacctgatc cgcgtccaga aactgcgcct catccaccag cacacaggcc agcggcccgc
     5401 gcgcctgttc ggctatccct tcggcaaaca ggtcggtatc tgcatcaaag cagcgggcat
     5461 cggcggcaag gccaatgcgg ctttcgatcg ccccctgccg cccgcgatca tccagcgctg
     5521 ctgtccacag catggtggcc atgccgcgct cgcgatagtt gaaatcggcc tgcagcagat
     5581 tggtcgattt gcccgcattc atgctggcat aatagaaata gagcttggcc acgccgcctc
     5641 agcccggctc gtcctcgccc tccacatcgc ggcgcacgcg cggatcggcc agcagcgcat
     5701 cgatgcggct ggcctcgtca aagctttcat ccggcaggaa cttgatgcgc gcggcatatt
     5761 tcagcttcag ccgcccggcc acttccttct ggaagaaggc ggtgttggtg cgcagcgcct
     5821 tcagcaccgc ctcctcatcc tcgcccagca gcggcttcac gaaaaccgcg gcatggcgca
     5881 agtccgggct catgcgcact tcggtcacgc tcacggcatg ggctgtcagc acatcgtcat
     5941 gcacctgctg gcgggccagc agttcggaga tcacatggcg cacctgctcg cccactttca
     6001 gcaggcgaac cgagcgggtt tcgggagtgg cggggctgcg ggccataact atccaatcga
     6061 ctaggtgatc gacaggccgc gcctggcggc acgcctgtcc ctgcccgccg ctttgccccc
     6121 gcaggcaggg gcttggcaat ggtttcctgc agggatttcc tgcgggggtt gcctgccggc
     6181 aaaggggttc cagccccgcc attcagaaaa ccgggtaagt tttctgaaca aaacccccca
     6241 aggccgttca gccaccactc atcgcaacag gggcaatttg ccctcatccc cgcaaggaga
     6301 gacaacatgc gaaagatcct caacactctg ctcgccgccg cactggtggc gcccatgctg
     6361 gcccccgccg ccgccatggc cgacccttat gaccacggcg gccccggctg gcacggcgat
     6421 cgcgaccatg gccctggccc cggcggcccg ggctggcatg gtgatcgcgg ccccggctgg
     6481 catggcgatc gcggctggca cgatggccgc ccgggcgact ggcgccagtt ccgccgcggc
     6541 gaacgcttcg accgcgaccg cgcctggcgc tatggcgagc tcgactatcg cgcctatcac
     6601 ggcctgcgcc gcccgccgcg cggctatcac tgggtgcgca atggcgatga cgcattgctg
     6661 atcggcatca ccagcggcat cgttgccagc gtgatcgtcg gcgccatccg ctaaccggcc
     6721 aaagggtgcg acacaccggc ggccccgtgc acagcggggc cgccttcttt ctggcatacc
     6781 ggcaacattg ccttatatca ttgcaaagcg gcaacattgc cgccctatcc ttcccccggc
     6841 ccggtggcat tcccccgcct gcgggcccat cagaaaggct caatcatgaa gcgtatcctt
     6901 tcggcggcca tcgccgttgc catgctcgcc ccggccgtgc cggccctggc ccagcccggc
     6961 ggcggcccgg atcgtggcgg cgaacatggc ggtgatcgcg gtggcgatca tggcggtgat
     7021 catggcggcg gcgagcgtgg cggcgatcat ggccgtggcg gccccggcgg cgatcatggc
     7081 ggcgcccccc agttccgccg tggcgagcat ttcgatcgcg gccgcgcagt caactatggc
     7141 gagctcgact atcgccagtt ccacggcatg cgccgcccgc cgcgcggcta tcactgggtg
     7201 cgcaatggcg acgatgcgct gctggtgggc atcaccagcg gcatcatcgc cagcgtgatc
     7261 gtcggcgcca cccgctaagc gctgcttcgg cgctatccat caggctggcg caaaaagaag
     7321 ggcggcctcc ttgc
//
LOCUS       DASRYE010000246         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_85677,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000246 DASRYE010000000
VERSION     DASRYE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85677"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFF98_12530"
     CDS             <1..297
                     /locus_tag="VFF98_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HZU64502.1"
                     /translation="SQHKNKAKAMQVLRARLYEKMRDEAQGAEAEARRAMVGSGDRSE
                     RIRTYNFPQGRVTDHRINLTLHRLPEVLEGPGLGELINALTAEDQAKRLAAIGE"
     gene            341..1207
                     /gene="prmC"
                     /locus_tag="VFF98_12535"
     CDS             341..1207
                     /gene="prmC"
                     /locus_tag="VFF98_12535"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZU64503.1"
                     /translation="MPANVGAAVRAAAAALAATSDTARLDAEWLMAHALGVSRSDLLL
                     RHMGDAVPAGFAALVARRAGHEPVAYILGHAEFYGLTLAVGPDVLIPRGDSETLIEAA
                     RLALEGRPPARILDLGTGSGCLLLAALSLWPQAEGVGVERSAGARAVAGANAQRLGLP
                     ARIIAADWTMAGWAADLGRFELILANPPYVEADAALAPDVAGHEPHSALFSGPEGLDD
                     YRLLIPQLPALLAPQGLALIEIGWQQAAAVAAIAHAAGLATRLHHDLAGRPRTIAAWA
                     SGAPWEEGAAQA"
     gene            1451..>2086
                     /locus_tag="VFF98_12540"
     CDS             1451..>2086
                     /locus_tag="VFF98_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HZU64504.1"
                     /translation="MTNNRGNNRRRGRGNGNNRPQGGGQQVNRIDSRARGNAPQLLEK
                     YRKLAHDAHLNGDRVQAEYYLQFADHYFRVIADTRLRQEEQRARREDRWQDNGEPYRD
                     DGEDGGEFSVESDFPAFDQPPVYTRREREDRPQREPRRSEAAAPAPAREEPVAEPGNE
                     AAEDADPRNIYEPAENPFVRENRTPRAAPRPRREREGREREERRPRRAERRE"
BASE COUNT          323 a          702 c          768 g          293 t
ORIGIN      
        1 agccagcaca agaacaaggc caaggccatg caggtgctgc gcgcccgcct ctatgagaag
       61 atgcgcgacg aggcgcaggg cgccgaagcc gaggcgcgcc gggcgatggt gggcagcggc
      121 gaccgttccg aacggatccg cacctacaac ttcccgcaag ggcgcgtgac cgaccaccgc
      181 atcaacctca ccctgcaccg cctgcccgaa gtgctggaag ggccgggcct gggcgagctg
      241 atcaacgcgc tgaccgccga ggatcaggcc aagcggctgg ccgccattgg cgagtgatct
      301 gcccccgggc gggcctgcgt ctgtaaacgc aggcgggcct gtgcccgcaa acgtcggcgc
      361 ggcggtgcgc gcggcggcgg cggcgctggc cgccaccagc gacaccgccc ggctggatgc
      421 cgaatggctg atggcccatg cgctgggggt ttcgcgctcg gatctgctgc tgcggcatat
      481 gggcgatgcg gtgccggccg gctttgccgc gctggtggcg cgccgggccg ggcatgagcc
      541 ggtggcctat attctgggcc atgccgaatt ctatggcctc accctggcgg tggggcccga
      601 tgtgctgatc ccgcgcgggg atagcgagac gctgatcgag gccgcccgcc tggcgctgga
      661 aggccggccc ccggcgcgca ttctcgatct gggcacgggc tctggctgcc tgctgctggc
      721 cgcgctcagc ctgtggccgc aggccgaagg ggtgggggtg gaacgttctg ccggcgcccg
      781 ggcggtggcc ggcgccaatg cgcagcggct gggcctgccg gcgcggatca tcgcggcaga
      841 ctggacgatg gccggctggg ccgcggatct gggccgcttc gagctgattt tggcgaaccc
      901 gccctatgtg gaggccgatg ccgcccttgc ccccgatgtg gccgggcacg agccgcacag
      961 cgcgctgttt tccgggccag aggggcttga tgattatcgc ctgctgatcc cgcagctgcc
     1021 ggccctgctg gcgccgcagg ggctggcgct gatcgagatc ggctggcagc aggcagcagc
     1081 agtggcggcc attgcccatg ccgccggcct tgccacgcgc ctgcaccatg atctggccgg
     1141 ccggccgcgg acgatagccg cctgggccag cggcgcccca tgggaagagg gcgcagcgca
     1201 ggcttaagaa tctgcttggc atttgctggt agggctttta aggagggttt gaggctggcg
     1261 agggatggct tttcccctct ggctggcccc actccagcat gagtgtcgga gattacggcg
     1321 atggtccgcc gtgagcttgg gcggatatag gcttggcgca ggctgaccgg gagctgccgc
     1381 gccgcactag gaacgcttag cctgtcggcg ctggagccgc acggcaagcc ggtttgagga
     1441 agacttaccc ttgaccaata atcgtgggaa taaccgtcgg cgcggccgcg gcaacggcaa
     1501 taacaggcct cagggcggtg gccagcaggt caaccggatc gacagccgtg cccgcggcaa
     1561 tgcgccgcag ttgctggaaa aataccgcaa gctggcgcat gatgcgcatc tgaacgggga
     1621 tcgcgtgcag gcggaatact acctgcagtt cgcagaccat tacttccgcg tgatcgccga
     1681 tacgcgcctg cgccaggaag aacagcgcgc ccgccgcgag gaccgctggc aggacaatgg
     1741 cgagccctat cgcgacgacg gcgaggatgg cggcgagttc tcggtggaga gcgattttcc
     1801 cgctttcgac cagccgcccg tctacacccg ccgcgagcgc gaggatcgcc cccagcgcga
     1861 gccgcgccgc agcgaggcgg cagcgcctgc ccccgcccgc gaggagcccg tggccgaacc
     1921 cggcaacgag gccgccgagg atgcggatcc gcgcaacatc tacgagccgg cggaaaaccc
     1981 cttcgtgcgg gagaaccgca cgccccgcgc cgcgccgcgt ccgcgccgcg agcgggaggg
     2041 ccgcgagcgt gaggaacgcc gcccccgccg cgccgagcgc cgcgag
//
LOCUS       DASRYE010000247        20440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_185001,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000247 DASRYE010000000
VERSION     DASRYE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20440
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185001"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFF98_12545"
     CDS             <1..956
                     /locus_tag="VFF98_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZU64505.1"
                     /translation="PPPGGEIPLWLVDRLFRHILVDITGNTHRAEICIDKLFSPDGPT
                     GRLGLVEFRGFEMPPEPRMSLAQQLLIRALAAMFWRKPVDGPLVRWGTQLHDRFMLPH
                     FVWADFLDVLTDLADAGYPMDPAWFEAQRQFRFPVHGAVHAGGVNLEITHALEPWNVL
                     GETGAIGGTVRYVDSSTERLQVKASGMVPGRHIITCNGRRVPLVPTGVPGEGVAGVRY
                     KAWWPANCLHPMLPPHAPLTFDVLDAWNGRSLGGCTYHVAHPGGRSYETEPVNAYEAE
                     ARRRARFAENAHSPGRWVVPAEELPNEFPFSLDLRRPAHVR"
     gene            966..3479
                     /locus_tag="VFF98_12550"
     CDS             966..3479
                     /locus_tag="VFF98_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZU64506.1"
                     /translation="MTNDAARSLPEDPADWLCSYPAGEGADLYRAAAPEVQRVWQAVA
                     RGLAQRSGPGRPVADQVARQIQELGLTFRLTGDDDERDWPLTPMPLIIGAKAWSALEK
                     GVIQRARLLNALAADILGPRRMVAEGHLPAAALAGSPYFARKMLGVVPASGHFLHTYA
                     VDLTLEADGRWRVLSDRLRLANGVGYALENRLAFSRATGSLLADVHTRRLATFFGELR
                     GGLARDCAREYPRIALLTPGRLNQSYPEQAHLARYLGFPLVEGRDLVVTDGRLYVRTI
                     AGPKRIDAVWRWLDTNALDPLAFDARSALGVPDLLRACAEGQLVMGNWPGVEVMESRA
                     MAAYLPRLCEILLREPLALPQVRTIWGGEGEHASALAERLDDWLVAPAFGQRASGLPG
                     PGAFAGSALDADQRAALLAAMALRPMDYCGQDMARLATTPVLVSNAEGAGALLPRPFS
                     LRVFVARGASGEWVVMPGGFARLCSGTQVQAALMGDSDLSADVCIVDPEPLARHARTA
                     LASEPAVQRGGGILASQAADNLFWLARYAERAETTLRILRSILGSSIDADGGLGQDPA
                     VTAALVRLLYLWGAISPRQAEAPLETICAAALAEPEMAGSVAFLLRRAQGVGQSLRQR
                     FSRDFWAMVARPMPRIDTSQPQAMLIAATELIERFSAISGQVAENMLRGPAWRFVDIG
                     RRLERALSVCRMVRQLAAIDSGVESGGDALNALLDLCDCPVTYRSRYLIGPARGPVLD
                     LLLLDPDNPRSLAYQAQALADHVAALPTLGESNLPEAPWRAARAILAPLETLRVEELK
                     DHVLQDIETRLLGLSEAISTRYFLPTERPEPRVQDSFLA"
     gene            3476..4456
                     /locus_tag="VFF98_12555"
     CDS             3476..4456
                     /locus_tag="VFF98_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZU64507.1"
                     /translation="MIYDVCHRTTIHYAGVVRLARFNLRLAPAPWPGQLVSNYRLVVD
                     PHPASLREAEGPYLAHECHLLLREPIARLTIESRFRAEVLERPVGPLPPGPPLAELRA
                     AALARRDLARTSPANYLFASPIARPDADIAAWAAAHIPPGCGVVAAGQALMQAIHAAF
                     RFDASATRADTSPTDAFRLRQGVCQDFTHVMIVAARAQGIPAAYVSGYLRTLPPPGMP
                     RLVGADAMHAWAALWCGDALGWVGFDPTNAVLAGTDHIFVAMGRDYSDVAPLDGVFHG
                     GDGQSMGVSVDVLPLGEEAGLGTEAGQGEDSNAPAQRQIPGFQRQRQAPA"
     gene            complement(4466..5308)
                     /locus_tag="VFF98_12560"
     CDS             complement(4466..5308)
                     /locus_tag="VFF98_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64508.1"
                     /translation="MSRALAAVAALIAVAAATPACAQASASDPSLYRDGQAPAAPLLR
                     PGQPAVLAPPPRIDHTGPLIARFTQAYRRAKSPRMVIFWNRALTDDVATSHELYARSH
                     TDSQANVQSGYGWAHGDSASNSETRLGISHDDPGRRDTPLAESDDFGLEQGFTDALAQ
                     AGVRLVDRTAIMRTGALRGGTWNLQALETRSILAHAELVVEVAPLADGSGGAGSFKVV
                     VRNLASGRLVANVQSRGEPPAQVQPYVAGPSGFVRAAPHEPTPYEAGRQIGLDVMAAL
                     AGRL"
     gene            complement(5305..5796)
                     /locus_tag="VFF98_12565"
     CDS             complement(5305..5796)
                     /locus_tag="VFF98_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64509.1"
                     /translation="MKTCRSMVAALVAGVLLPATLLPASLQASENRPLKCDIAEARSA
                     APTGGPALVANVPGAMTPIDLDAVLMDDKGVWKKVVVEGLYARRTPTNTVSVSARLVN
                     CSKQPITIQARSSFLDAAQGPAEPASVWKVIHISPRSIGTYQESSLSTGVANYLIELR
                     SDQ"
     gene            complement(5793..7331)
                     /locus_tag="VFF98_12570"
     CDS             complement(5793..7331)
                     /locus_tag="VFF98_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64510.1"
                     /translation="MQKSMFLLGVAGAVLMVPAAAEAQFGLLGSMLAQKRSKAAVPAA
                     NAAATPTPVASGAPAAATGAPASTPTPVPTVEALPTQADIGAADVNPADTPAGSFAGS
                     RPQPLRPIYRALYNGGERNAVLNFERLGLAALQMGDYSRAEWAFDQALNRIEAIYANN
                     PQAATARSVFHNEANKDFKGEPYERAMAYYYRGLLYLRVGDFSNARASFKMAEYQDTL
                     SDSESFESEFAVMDYLVGWTLRCDGQGATADESFAIAAKAQAGLAAPPPTDNVLFIAE
                     IGNGPQKLRDGAQAQKLLFRAGPAYPETTVAVSLAAPARKAAPGRPVAKPAAASAHPI
                     PLMEAGSVSYQATTRGGRAIDGLMNGKANWKSGTDTVGNVALIAGASGMFGNDAGGSL
                     GMMGFGLAMKMFSSGMKTNADIRAWDGLPDKILVATAHAPAPDWTYAASYRAGEVALP
                     FRDAQMRADPKGCSVVWLRARPAAYGEAIVGEDAGVAASVAKKKQVQEKNTRFRAQLE
                     ESKL"
     gene            complement(7442..8062)
                     /locus_tag="VFF98_12575"
     CDS             complement(7442..8062)
                     /locus_tag="VFF98_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HZU64511.1"
                     /translation="MNYSAALALLAAGGVAHAGDNRHGTQAMELDPGSKGAIGGIGIE
                     SRDIDRIADQVVRELMARPDLVNTPVPPRVVVDSEKFYNNSAQRIDRDMITGALRASL
                     NRAAAGRIRFVSRESMDIVMRERELKRAGTVDVGTRGLTRAASGVDYQLIGKMTSLDS
                     RDGRSGLMQRRTQVIFELVDLETGDLPWTSEPYVILRAAGDDIIYR"
     gene            complement(8370..8444)
                     /locus_tag="VFF98_12580"
     tRNA            complement(8370..8444)
                     /locus_tag="VFF98_12580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8410..8412),aa:Thr,seq:ggt)
     gene            8597..8848
                     /locus_tag="VFF98_12585"
     CDS             8597..8848
                     /locus_tag="VFF98_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64512.1"
                     /translation="MTTPPAPPQTPPADPRAEHTDARAERLAAKLRENLHRRKAQARA
                     LSEEDARALSEEDARALSEDDARALGGGEAPSAPPPATP"
     gene            8958..9644
                     /gene="gpmA"
                     /locus_tag="VFF98_12590"
     CDS             8958..9644
                     /gene="gpmA"
                     /locus_tag="VFF98_12590"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZU64513.1"
                     /translation="MPRLILVRHGQSQWNLENRFTGWWDVDLTEKGEAEARAAGTLLK
                     DKGLLPTVAFTSFQTRAIRTLHLALEAAGRVWIPEYKDWRLNERHYGGLTGLDKAETA
                     ALHGDEQVKIWRRSFDVPPPVLEAGSAFDLKQDPRYAGIAIPDTESLKLTIARVLPYY
                     EAAIVPQLQAGETVIVAAHGNSLRALVKHLSGISDADIVSLEIPTGQPIVYELDDSLA
                     ATDRYYLSER"
     gene            9649..10143
                     /gene="purE"
                     /locus_tag="VFF98_12595"
     CDS             9649..10143
                     /gene="purE"
                     /locus_tag="VFF98_12595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZU64514.1"
                     /translation="MTRPGSLVSIVMGSQSDWHTMKKAADILTQLGVAHDVRIVSAHR
                     TPDRLVEYAKSAADEGFKVIIAGAGGAAHLPGMVASMTHLPVLGVPVQSKALSGKDSL
                     LSIVQMPAGVPVGCLAIGEPGATNAGLLAAKILATSDAALAARVKAFRTEQTEAVAER
                     PVEG"
     gene            10154..11209
                     /locus_tag="VFF98_12600"
     CDS             10154..11209
                     /locus_tag="VFF98_12600"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZU64515.1"
                     /translation="MIPPGETIGILGGGQLGRMLAMAAARLGYRVHVFAPEADSIAAD
                     VAAEHTCAEWHDTEALARFAAACAVITYEFENVPVGPLAALGLVPLLPGPRALETAQD
                     RLAEKRFVQALGGTPAPFMAVDGADDLARAIAEIGTPGILKTRRDGYDGKGQWRIMTP
                     ADAEGLELPPKPLIYEGFVSFGHEFSVILARGGDGGIVFWDSADNIHKAGILDITRVP
                     APEVITQQVPAARALAQKVAEALDYVGVLTLEFFATAAGPVFNEMAPRVHNSGHWTIE
                     GALTSQFENHIRAICGLPLGATTLAVQGAEMRNLIGDEAHDWPAILADPANHLHLYGK
                     AAARPGRKMGHVTRLSL"
     gene            11206..11697
                     /locus_tag="VFF98_12605"
     CDS             11206..11697
                     /locus_tag="VFF98_12605"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZU64516.1"
                     /translation="MSGEIVIVVARAANGVIGNQGAMPWHLPADLRHFKALTMGKPMI
                     MGRKTFASLPGLLPGRRHIVLTRDAGWAAPGAETAADVAGALALAGDVPEICVVGGGE
                     VYAQFLPLADRIEITHIDAEPAGDTHFPALAGPPWHEAARTEHPAQEGRPAFAFVTLT
                     RSP"
     gene            11708..12637
                     /locus_tag="VFF98_12610"
     CDS             11708..12637
                     /locus_tag="VFF98_12610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZU64517.1"
                     /translation="MIRLDSRLPIPDALRGAIFALGNFDGFHLGHQAVVGEALAWARA
                     EGRPAIVATFDPHPVSVFVPDAPPFRLTTLDQREELFGAAGADAMLVFHFDAAMAATP
                     ADGWVADILGRQLGAAGVVTGEDFTFGKGRGGNVGLLAEMGAAHGIGARAVGPVRDAG
                     GVISSSRIREAIAAGDMAAAARLLSRPFAIRGHVQHGDKVGRTIGFPTANLDMADYLR
                     PAYGIYAVTGRLPDGRVLKGAANLGIRPTFTPPKELLEPHFFDFSGDLYGQQIEVAFH
                     AFLRGEAKFGSLDALMQQIGRDCEKAREILESI"
     gene            12692..15565
                     /gene="ileS"
                     /locus_tag="VFF98_12615"
     CDS             12692..15565
                     /gene="ileS"
                     /locus_tag="VFF98_12615"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZU64518.1"
                     /translation="MTEKRDYRDTVFLPKTDFPMKAGLPQKEPGILARWQQQGLYRAL
                     REARAGAEKFILHDGPPYANGNMHIGHAVNHILKDMVVRSQSLLGKDAPYVPGWDCHG
                     LPIEWKVEEEYRKKKKNKDEVPPKEFRAECRAYAQTWVNAQREQLKRLGINGDWDNPY
                     LTMDFQAEATIVGELLKFATSGQLYRGAKPVMWSPVEKTALAEAEVEYEDITSTQIDV
                     AFEIVESPIAELVGAYAVIWTTTPWTIPANQALAYGPEVDYHLYRADDGRRFLIAVDL
                     LGHFLARTGLGPHPEGLTGGLDLAEHALDTIKGADLAGTIARHPMAAAFPDSAFFTRP
                     RPFLAGDFVTTESGTGLVHMAPDHGEDDFALCKAHGIEPVFAVEGDGKYRADWPWLGG
                     EGSVINPKFNAPDGPICTALREAGSLLAASADYKHSYPHSWRSKAKVIFRCTPQWFVP
                     MDKPIDRQIPLCATEAAIRTEQHAGETLREIAVAAIAATRFVPEKGRNRIGAMVEGRP
                     DWVLSRQRAWGVPITLFVDRKTGQYLADPAVNARIIAAIREEGVDAWDEARAQEFLGA
                     DYNAADYERVTDILDVWFDSGSTHAFVLESGRWPDLTRPAGHQGPWADLYLEGSDQHR
                     GWFQSSLLESCATRGHAPYKAILTHGFTMDAKGLKMSKSLGNTVDPLKVMETYGADII
                     RLWALSVDYTEDHRIGDEILKGVADQYRKLRNTFRYLLGALDGFAEEERLPVAEMPEL
                     ERYVLALLGGLDATLKQAIADFDFNAYVRALVDFCNEDLSAFFFDIRKDVLYCDAPAS
                     LKRRAYRTVLDILFHALIRYAAPVLVFTAEEVWAARYPGAQSVHLLEWPDVPAVDVAA
                     ERWAALRALRAEVLGAIEPLRRDKTLGSSLEAEVTVPADAPEGDLAELFISASVARGE
                     GLAVARTEAHKCGRCWRHLPEVSEDGDLCGRCEETVALVDAGR"
     gene            15562..16080
                     /gene="lspA"
                     /locus_tag="VFF98_12620"
     CDS             15562..16080
                     /gene="lspA"
                     /locus_tag="VFF98_12620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZU64519.1"
                     /translation="MKGARARLLGLAVAGLVFLADQAVKAAVTGPLALPEIGQIRLLP
                     IFNLTWTQNFGVSLGLLTASSDQQRWLLVGITAAIALFVCAWLWREKRGLDAGALGLI
                     LGGALGNIRDRITAGFVIDYADLHFGDFRPFLVFNLADAAISLGVLIILARSFLSREK
                     RGAETAHSATEM"
     gene            16087..16500
                     /locus_tag="VFF98_12625"
     CDS             16087..16500
                     /locus_tag="VFF98_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HZU64520.1"
                     /translation="MRKMNALVLAGATTVLLSGCSVGNLFNRVRPDEFAVQRQAPLMV
                     PPDYSLAPPQPGAPRPATRTAEQETLEAMFGGPAPRSGVEKTVDGKAGTAEPSIRSTV
                     ADPKTATANKGDVTKDILAAPQGDGREAQARINGS"
     gene            complement(16580..17884)
                     /locus_tag="VFF98_12630"
     CDS             complement(16580..17884)
                     /locus_tag="VFF98_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZU64521.1"
                     /translation="MTPFPWIDVLVIAGLVLLNGLFAMSELAIVSARPARLRMAADRG
                     HRGSRLALALAEDPGKLLSTVQIGITLVALATGAFSESSLGAPVAERIGGWGLSPTLA
                     DRLASVLVLGGTTLVSLIVGELVPKQLALRHAELIAGLAAPVMAVMARGMAPLVWFLD
                     RSSRALLRLFGEKDDDEPQVTAEEIHLMFAEATRSGVIEEEERAIMTGIMRLADRPVR
                     ELMTPRNQIDWIERDASPQDLRVAITESPHSLLAVADGSPDTIVGVVKAREVQAALLQ
                     GDAPDIGALMKKAEIVPDQLDAMDALRILQQSEVAMALVHDEYGHLEGIVTPTDLLTA
                     ILGDFASDRDEGDAPMLVERGDGSLLISGSMPADALAGRLGLDLPGSRDYATAAGYAL
                     SVLRRLPDEGDSFADQDWRFEIVDMDGRKIDKLLVSPLKGTA"
     gene            complement(17950..18768)
                     /locus_tag="VFF98_12635"
     CDS             complement(17950..18768)
                     /locus_tag="VFF98_12635"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZU64522.1"
                     /translation="MIDRERLEQIVRAAGALALKAWPGQGPWASPRENPLKTWYKSDG
                     QPVCEADMAVDAFLRAELGALLPQAGWLSEETVDSPGQRDGDLIWLVDPIDGTRDFVA
                     GRDGWAVSVALISCGRPLIGLLHAPARGNGGGGELWLAEEGRGATRDGRRLRASTRAD
                     LAGARVPARLLGPEDADLTPVSQPNSIALRIAMVAADEADLLATLRWGYEWDIGAACL
                     IAREAGAAVSDAFGQALGYNKPDPRAFGVLVSAPAIHAAAVARLGPRARHIAGA"
     gene            18903..20105
                     /gene="sucC"
                     /locus_tag="VFF98_12640"
     CDS             18903..20105
                     /gene="sucC"
                     /locus_tag="VFF98_12640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZU64523.1"
                     /translation="MNIHEYQAKELLAKFGVAIPAGHAALTVEEAVAGAKLLPGPLYV
                     VKSQIHAGGRGKGKFKELPADAKGGVRLAKSLEEVESHSQEMLGNTLVTIQTGEAGKQ
                     VNRLYVTDGVDIAHEYYLSLLVDRKTGRVAIIASTEGGMDIEEVAHSTPEKITTITID
                     PASGFQPHHGRAIAFALKLKGDTNKQAQVLAKQLYDAFVALDCAMLEINPLVEDKSGA
                     LLVLDTKMSFDSNALYRHPDVQALRDETEEDPAEIEASKYDLAYIKLDGNIGCMVNGA
                     GLAMATMDIIKLNGAFPANFLDVGGGATTEKVTAAFKIILSDPAVEGILVNIFGGIMK
                     CDVIAEGIIAAAKDVNLSVPLVVRLEGTNVEKGKELLNNSGLPIVAANDLGDAAKKIV
                     AEVAKVSA"
     gene            20301..>20440
                     /locus_tag="VFF98_12645"
     CDS             20301..>20440
                     /locus_tag="VFF98_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZU64524.1"
                     /translation="MKILVPVKRVIDYNVKPRVKADGTGVDLSNVKMSMNPFDEIAVE
                     EG"
BASE COUNT         3250 a         7003 c         6886 g         3301 t
ORIGIN      
        1 tgccgccgcc gggcggggag atcccgctgt ggctggtcga tcgcctgttc cggcacatcc
       61 tggtcgacat caccggcaac acccaccgcg ccgagatctg catcgacaag ctgttctcgc
      121 ccgatggccc caccggccgg ctggggctgg tggaattccg cggcttcgaa atgccgccag
      181 agccgcggat gagcctggcc cagcaattgc tgatccgcgc gctggcggcc atgttctggc
      241 gcaagccggt ggatgggccg ctggtgcgct ggggcacgca gctgcatgat cgcttcatgc
      301 tgccgcattt cgtctgggcc gatttcctcg atgtgctgac cgatcttgcc gatgccggct
      361 acccgatgga cccggcctgg ttcgaggcgc agcgccagtt ccgcttccct gtgcatggcg
      421 cggtgcatgc cggcggcgtg aacctggaaa tcacccatgc gctggaaccc tggaacgtgc
      481 tgggcgagac cggcgcgatc ggcggcacgg tgcgctatgt cgacagttcg accgagcggc
      541 tgcaggtgaa ggccagcggg atggtgcccg gccgccacat catcacctgc aacggccggc
      601 gggtgccgct ggtgcccacc ggcgttccgg gcgaaggggt ggccggcgtg cgctacaagg
      661 cgtggtggcc ggccaattgc ctgcatccga tgctgccgcc ccatgccccc ctcaccttcg
      721 acgtgctgga tgcctggaac gggcgcagcc tgggcggctg cacctatcat gtggcccacc
      781 cgggcgggcg cagctatgaa acagagccgg tgaacgccta tgaggccgag gcccgccgcc
      841 gcgcccgctt tgccgaaaac gcccattcgc cgggccgctg ggtggtgccg gcggaagagc
      901 tgcccaacga attccccttc tcgctggatt tgcgccggcc cgcacatgtg cgataatccg
      961 cgccgatgac caatgatgcc gcccgcagcc tgcccgaaga tccggcggac tggctgtgtt
     1021 cctatcccgc cggcgagggg gcggatctct atcgcgccgc cgcgcccgag gtgcagcgcg
     1081 tgtggcaggc ggtggcgcgc gggttggcgc agcggtcggg ccccgggcgc ccggtggccg
     1141 atcaggtggc ccgccagatc caggagctgg gcctcacctt ccgcctgacg ggcgatgacg
     1201 acgagcgcga ctggccgctg acgccgatgc cgctgatcat cggcgccaaa gcgtggagcg
     1261 cgctggaaaa gggggtgatc cagcgggcgc gcctgctcaa tgcgctggcc gccgatattc
     1321 tgggcccgcg ccgcatggtg gcagaaggcc atctgcccgc cgccgcgctg gccggcagcc
     1381 cctatttcgc ccgcaagatg ctgggggtgg tgccggcctc gggccatttc ctccacacct
     1441 atgcggtgga cctgacgctg gaagccgacg ggcgctggcg cgtgctgtcg gaccggctgc
     1501 ggctggccaa tggcgtgggc tatgcgctgg agaacaggct cgccttctcg cgcgccaccg
     1561 gctcgctgct ggccgatgtg cacacgcggc gcctcgccac cttctttggc gagctgcgcg
     1621 gcggcctggc ccgcgattgc gcgcgcgaat atccgcgtat tgccctgctc acccccggcc
     1681 ggctgaacca gagctatccc gaacaggcgc atctcgcccg ctatctgggc tttccgctgg
     1741 tggaggggcg cgatctggtg gtgacggacg ggcggcttta tgtgcgcacc attgccggcc
     1801 ccaagcggat cgatgcggtg tggcgctggc tggataccaa cgcgctcgat ccgctggctt
     1861 ttgatgcgcg ctctgcgctg ggggtgccgg atctgctgcg cgcctgcgcc gaagggcagc
     1921 tggtgatggg caactggccg ggggtggagg tgatggaaag ccgggcgatg gcggcctatc
     1981 tgccgcgcct gtgcgagatc ctgctgcgcg aaccgctggc cctgccgcag gtgcgcacga
     2041 tctggggcgg cgagggcgaa catgccagcg ccctggccga gcggctggac gactggctgg
     2101 tggcgcccgc ctttggccag cgggccagcg gcctgcccgg ccccggcgcc tttgccggca
     2161 gcgcgctgga tgccgaccag cgcgccgccc tgctggccgc catggcgctg cggcccatgg
     2221 attattgcgg gcaggatatg gcccggctgg ccaccacgcc ggtgctcgtc agcaatgccg
     2281 aaggggccgg cgcgctgctg ccgcggccgt tctcgctgcg ggtgttcgtg gcgcgggggg
     2341 catcgggcga atgggtggtg atgccgggcg gctttgcccg cctgtgttcg ggcacgcagg
     2401 tgcaggccgc gctgatgggc gacagcgacc tgtccgccga tgtctgcatc gtcgatcccg
     2461 agccgctggc ccgccatgcc cgcaccgcgc ttgccagcga gccggccgtg cagcgcggcg
     2521 gcggcattct cgccagccag gcggccgaca atctcttctg gctggcccgc tatgccgagc
     2581 gggcggaaac cacgctgcgc atcctgcgct ccatcctggg cagctcgatc gatgccgatg
     2641 gcgggctggg gcaggatccg gccgtgacgg cggcgctggt gcggctgctc tatctgtggg
     2701 gcgcgatcag cccgcgccag gccgaggcgc cgctcgagac gatctgcgct gccgccctgg
     2761 ccgagcccga gatggccgga tcggtggcct tcctgctgcg ccgggcgcaa ggggtggggc
     2821 aatcgctgcg ccagcgcttc tcgcgcgatt tctgggcgat ggtggcgcgc cccatgccgc
     2881 gcatagacac cagccagccg caagccatgc tgatcgccgc gaccgagctg atcgagcggt
     2941 tttccgccat ttccggccag gtggccgaga acatgctgcg cgggccggcc tggcgcttcg
     3001 tcgatatcgg ccggcggctg gagcgggcgc tgtcggtctg ccggatggtg cgccagctgg
     3061 ccgcgatcga cagcggggtg gaaagcggcg gcgatgcgct gaacgccctg ctcgacctgt
     3121 gcgattgccc ggtcacctat cgctcgcgct atctgatcgg gccggcgcgc gggccggtgc
     3181 tcgatctgct gctgctcgat ccggacaatc cgcgctcgct ggcctatcag gcgcaggcgc
     3241 tggccgatca tgtcgcggcg ctgccaacgc tgggtgaaag caacctgccc gaagccccct
     3301 ggcgcgccgc ccgggccatt ctggcgccgc tggaaacctt gcgcgtggaa gagctgaagg
     3361 accacgtgct gcaggatata gaaacacgcc tgctgggctt gtcggaagcg atctcgacgc
     3421 gctattttct gccgaccgag cggcccgagc cgcgcgtgca ggacagtttc ctcgcatgat
     3481 ctacgatgtc tgccaccgca caaccattca ctacgccggc gtggtgcggc tggcgcggtt
     3541 caacctgcgg ctggcgccgg cgccctggcc ggggcaattg gtcagcaatt accggctggt
     3601 ggtcgatccg catccggcca gcctgcgcga ggcggaaggg ccctatctgg cccatgaatg
     3661 ccaccttttg ctgcgcgagc cgatcgcccg gctgaccatc gaaagccgct ttcgcgccga
     3721 agtgctggag cggccggtgg gcccgctgcc ccccgggccg ccgctggcag agctgcgcgc
     3781 ggccgccctt gcccggcggg atctggcccg caccagcccc gccaactacc tcttcgcctc
     3841 gcccatcgcc cggcccgatg ccgatatcgc cgcctgggcc gccgcgcata ttccgcccgg
     3901 ctgcggcgtg gtggcggcgg gccaggcgct gatgcaggcg atccacgccg ccttccgctt
     3961 cgatgccagc gccacccgcg ccgacacctc gcccaccgat gccttccgcc tgcgccaggg
     4021 cgtgtgccag gattttaccc atgtgatgat cgtggccgcc cgcgcgcagg gcataccggc
     4081 ggcctatgtc agcggctatc tgcgcacgct gccgccgccg ggcatgccgc ggctggtggg
     4141 ggccgatgcg atgcatgcct gggcggcgct gtggtgcggc gatgcgctgg gctgggtggg
     4201 tttcgatccc accaatgcgg tgctggccgg caccgatcat atcttcgtgg cgatgggccg
     4261 cgactatagc gatgtcgccc cgctggacgg ggtgttccat ggtggcgacg ggcaatcgat
     4321 gggcgtcagc gtcgatgtgc tgccgctggg cgaggaagcg gggctgggca ccgaagcggg
     4381 ccagggcgag gacagcaatg cccccgccca gcgccagatc cccggcttcc agaggcagag
     4441 gcaggcccct gcctgaagcc ggcgcctaaa gccggccggc cagcgccgcc atcacatcca
     4501 gcccgatctg ccgcccggcc tcatagggcg tgggctcatg cggggcggcg cgcacaaagc
     4561 cgctgggccc ggccacatag ggctgcacct gcgccggcgg ctcgccccgg ctttgcacat
     4621 tggccaccag ccgcccgctg gccagattgc gcaccaccac cttgaacgag cccgccccgc
     4681 cgctgccatc ggccagcggc gccacttcca ccacgagttc ggcatgggcc aggatgctgc
     4741 gcgtttccag cgcctgcagg ttccacgtgc cgccgcgcag ggcgccggtg cgcatgatcg
     4801 ccgtgcgatc caccagccgc acgccggcct gcgccagcgc atcggtaaag ccctgttcca
     4861 gcccgaaatc atcgctttcg gccagcggcg tgtcgcgccg gccggggtcg tcgtggctga
     4921 tgcccaggcg cgtttcgctg ttgctggcgc tgtcgccatg ggcccagcca tagccgcttt
     4981 gcacattggc ctggctgtcg gtgtggctgc gggcatagag ttcgtggctg gtggccacgt
     5041 catcggtcaa cgcgcggttc cagaagatca ccatgcgcgg cgatttcgcc cggcgatagg
     5101 cctgggtgaa gcgggcgatg agcgggccgg tgtggtctat ccgcggcggc ggggccagca
     5161 cggcgggctg gccggggcgc agcagcgggg cggcaggcgc ctgcccgtca cggtagagcg
     5221 agggatcgct ggcggaggcc tgcgcgcagg cgggcgtggc ggcggccacg gcgatgaggg
     5281 cggccacggc ggctagggcg cggctcattg gtcgctgcgc agttcgatga ggtagttcgc
     5341 aacgcccgtg ctgagcgaac tttcctgata ggtgccgatc gagcgcggcg agatgtggat
     5401 caccttccac accgaagccg gctctgccgg gccttgcgcg gcatcgagga aggaggaacg
     5461 cgcctggatg gtgatcggct gcttggagca gttcaccagc cgcgcgctga ccgacaccgt
     5521 gttggtgggg gtgcgccggg catagagccc ttcaaccacc accttcttcc acacgccctt
     5581 gtcgtccatc agcaccgcgt ccagatcgat cggcgtcatc gcgccgggca cattggccac
     5641 cagcgccggc ccgcctgtgg gcgcggccga gcgcgcctcg gcgatatcgc atttgagcgg
     5701 ccggttctcg gatgcctgca gggaggctgg cagcagggtg gctggcagca gcacccccgc
     5761 caccagcgcc gccaccatcg aacgacatgt cttcacagct tgctctcctc gagctgggcc
     5821 cggaaccggg tgttcttttc ctgaacctgc ttcttcttgg ccaccgaggc ggcaacgccg
     5881 gcatcctcgc cgacgatcgc ctcgccatag gcggccgggc gggcgcgcag ccacaccacc
     5941 gaacagccct tggggtctgc ccgcatctgc gcatccctga agggcagcgc cacctcgccc
     6001 gcgcgatagc tggcggcata ggtccagtct ggcgcggggg cgtgggcggt ggccaccagg
     6061 atcttgtcgg gcaagccatc ccaggcgcgg atgtcggcgt tggtcttcat gccagaggag
     6121 aacatcttca tcgccaggcc aaagcccatc atccccaacg agccgcccgc atcattgccg
     6181 aacatgccgc tggcgccggc gatcagcgcc acattgccca ccgtgtcggt gcccgatttc
     6241 cagttggcct tgccgttcat cagcccgtcg atcgcgcggc cgccacgggt ggtggcctga
     6301 tagctgacgc tgcccgcttc catcagcggg atcggatggg ccgaggctgc cgccggcttg
     6361 gccacaggcc ggcccggcgc agccttgcgc gcgggcgctg ccaggctgac ggcaaccgtc
     6421 gtttccggat aggcgggccc ggcgcggaac agcagctttt gcgcctgcgc cccatcgcgc
     6481 agcttttgcg ggccattgcc gatctcggcg atgaacagca cgttgtcggt gggcggcggc
     6541 gcggcaaggc cggcctgggc cttggcggca atggcgaagg attcatcggc ggtggcgccc
     6601 tgcccgtcgc agcgcaaggt ccagccgacg agatagtcca tcaccgcaaa ttcgctctcg
     6661 aagctttccg aatcagagag cgtgtcctga tattcggcca tcttgaacga ggcgcgggca
     6721 ttgctgaaat cgcccacccg caaatagagc aggccgcgat agtaataggc catggcgcgt
     6781 tcatagggct cgcccttgaa atccttgttg gcctcgttgt ggaacaccga gcgggcggtg
     6841 gcggcctgcg ggttgttggc gtagatcgcc tcgatgcggt tcagcgcctg gtcgaacgcc
     6901 cattcggcgc gcgaataatc gcccatctgc agcgcggcca ggcccagccg ctcgaaattg
     6961 agcaccgcat tgcgctcgcc gccattatac agcgcgcggt agatcgggcg cagcggctgc
     7021 gggcggctgc cggcaaagct gccggcgggc gtgtcggccg ggttcacatc ggccgcgccg
     7081 atatcggcct gggtgggcag ggcttccacc gtgggaaccg gcgtgggcgt ggatgcgggc
     7141 gcgcccgtgg cggcggctgg cgcgccgctg gccaccggcg ttggcgtggc cgcggcattg
     7201 gccgccggca cggccgcctt gctgcgcttc tgggccagca tgctgcccag caggccaaac
     7261 tgcgcttcag ccgcggccgg caccatcagc acggcgcccg ccacacccag caggaacatg
     7321 gatttctgca tgataacacc cccttgttat cgcgcggcct ggcgcccccc gtggccatcc
     7381 agaaccgcga atatctgtgc aaacacccgg tgcggcaccc ctgccgaccg gggcctgccg
     7441 atcaccggta gatgatgtcg tcgccggcgg cccgcaggat cacatagggc tcgctggtcc
     7501 agggcagatc gccggtttcc agatcgacca gttcgaagat cacctgggtg cggcgctgca
     7561 tcagcccgct gcggccatcg cgcgaatcga gcgaggtcat cttgccgatc agctggtaat
     7621 cgacgcccga agccgcgcgg gtgaggccgc gcgtgccgac atccaccgtg cccgcccgct
     7681 tcagctcgcg ctcgcgcatg acgatatcca tcgattcgcg gctgacgaag cggatgcgcc
     7741 cggccgctgc ccggttgagg ctggccctga gcgcgccggt gatcatgtcg cgatcaatcc
     7801 gctgcgcgct gttgttgtag aatttctcgc tgtcgaccac cacgcgcggc ggcaccggcg
     7861 tgttcaccag atcggggcgg gccatcagct cgcgcaccac ctggtcggca atccggtcga
     7921 tatcgcgcga ttcgatgccg atgccgccga tcgccccctt gctgccaggg tccagctcca
     7981 tcgcctgggt gccgtggcgg ttatcgccgg catgcgccac gccgcctgca gcaagcagcg
     8041 ccaaggcagc ggaataattc aagaatttcg ccatcgttcc ccctcgattg cgtttcccgg
     8101 caagcgcccc cgcgccagat ctgggaaata agatcaccgg acaatgatta aggcataata
     8161 tttcagtatt atgccagatc tgcacggatg ttgataaggg ccgatacgag attttgtcaa
     8221 tcctgccctt tgctccggtt tttcttggcg aaaaggctgc gaaaaaaccc tgaccgcagc
     8281 catggggcga ataaagcggc aggggcaaag gcccggatcc aaatcgaaaa aggggaaagc
     8341 cagcgcgaca gggacggacg ggagatctgt ggtgctgctg gggaggattg aactcccgac
     8401 ctcagcctta ccaaggatgc gctctaccac tgagctacag cagcgtgacc acagatctca
     8461 tccgtcgctc ggccaaacca gtcggcgtcg cggtggaggc gcgctattgg ccggggaaag
     8521 cgtgcttgtc aagcgcctgt gatgcagttg tcgcttgagc tggccgcgcg caggcggcac
     8581 aaggcccgcc acagccatga caacgccccc cgcccccccg caaaccccac ccgccgatcc
     8641 ccgcgcagag cacaccgatg cccgcgcaga gcgcctggcc gccaagctgc gcgagaacct
     8701 gcaccgccgg aaggcccagg cccgcgcctt gagcgaagag gatgcccgcg ccttgagcga
     8761 ggaagacgcc cgcgccttga gcgaagacga cgcccgcgcc ttgggcgggg gcgaggcccc
     8821 ctcggccccg ccgcccgcca cgccgtaacg ccctttccaa agcgcgcccg aaaagctagg
     8881 gccctgcctt tgccgaagca cacaccccgg cgcccccgcc ccgcagcggg ccgcgcccaa
     8941 agaggagagt gacaccgatg cctcgtctga tcctggtccg ccatggccag agccagtgga
     9001 acctcgaaaa tcgcttcacc ggctggtggg atgtggacct gacggaaaag ggcgaggccg
     9061 aggcccgcgc tgccggcacc ctgctgaagg acaagggcct gctgccgacg gtggccttca
     9121 cctccttcca gacccgcgcc atccgcacgc tgcatctggc gctggaagcg gccgggcggg
     9181 tgtggatccc cgaatacaag gactggcgcc tgaacgagcg ccattatggc ggcctcaccg
     9241 ggctcgacaa ggccgagacg gcagccctgc atggcgacga gcaggtgaag atctggcgcc
     9301 gcagcttcga tgtgccgccg ccggtgctgg aagcggggag cgctttcgat ctgaagcagg
     9361 atccgcgcta tgccggcatc gccattcccg ataccgaatc gctgaagctg accatcgcgc
     9421 gcgtgctgcc ctattatgaa gccgccatcg tgccccagct gcaggccggc gagacggtga
     9481 tcgtggctgc ccatggcaat tcgctgcgcg cgctggtgaa gcacctgtcg ggcatttcgg
     9541 atgcggatat cgtcagcctc gagatcccca ccggccagcc gatcgtctat gagctggacg
     9601 acagcctggc tgccaccgac cgttattacc tgtctgaaag gtaagctgat gacccgtcct
     9661 ggttcgcttg tttcgattgt catgggttcc cagtccgact ggcacaccat gaagaaggcc
     9721 gcagacatcc tcacccagct gggcgtggcg catgatgtgc gcatcgtctc ggcgcatcgc
     9781 acgcccgatc ggctggtgga atatgccaag agcgcggccg atgaaggctt caaggtgatc
     9841 atcgccggcg ctggcggcgc ggcgcatctg ccgggcatgg tggccagcat gacgcatctg
     9901 ccggtgctgg gcgtgccggt gcagtccaag gcgctgtcgg gcaaggattc gctgctgtcg
     9961 atcgtgcaga tgccggccgg cgtgccggtg ggctgcctgg cgattggcga gccgggggcc
    10021 accaatgccg gcctgctggc cgccaagatc ctggccacca gcgatgcggc cctggccgcg
    10081 cgggtgaagg ccttccgcac cgagcagacc gaagcggtgg ccgaacggcc ggtggaaggc
    10141 tgaaaggcaa gcgatgatcc cccccggcga gacgatcggc attctgggcg gcggccagct
    10201 ggggcggatg ctggccatgg cggcggcgcg gctgggctac agggtgcatg tcttcgcgcc
    10261 cgaggctgac agcatcgccg ccgatgtggc ggccgaacac acctgcgccg aatggcacga
    10321 taccgaagcg ctggcccgct ttgccgcagc ctgcgcggtg atcacctatg agttcgagaa
    10381 tgtgccggtg gggccgctgg cggcgctggg gctggtgccc ctgctgccgg gcccccgcgc
    10441 gctggaaacc gcgcaggaca ggctggccga gaagcgcttt gtgcaagcgc tgggcggcac
    10501 gccggcgccc ttcatggcgg tggatggcgc cgacgacctc gcccgcgcca ttgccgaaat
    10561 cggcacgccc ggcattctga aaacccggcg cgatggctat gacggcaagg gccagtggcg
    10621 gatcatgacc ccggccgatg ccgaggggct ggaactgccg ccaaagccgc tgatctacga
    10681 gggctttgtc agcttcggcc acgaattctc ggtgattctg gcgcgtggcg gcgatggcgg
    10741 gatcgtcttc tgggattcgg ccgacaatat ccacaaggcc ggcattctcg acatcacccg
    10801 cgtgcccgcg cccgaagtga tcacgcagca ggtgccggcc gcccgcgcgc tggcgcagaa
    10861 ggtggcagaa gcgctcgatt atgtcggcgt gctgacgctg gaattctttg ccacggcagc
    10921 tgggccggtg ttcaacgaga tggcgccgcg cgtccacaat tccggccact ggacgatcga
    10981 gggcgcgctc accagccagt tcgagaacca tatccgcgcc atttgcggcc tgccgctggg
    11041 cgccaccacg ctggccgtgc aaggcgccga aatgcgcaac ctgatcggcg atgaggcgca
    11101 tgactggccg gcgatcctgg ccgatccggc caaccatctg catctctatg gcaaggccgc
    11161 ggcccgcccc gggcgcaaga tgggccatgt gacccgcctc agcctgtgag cggcgagatc
    11221 gtcatcgtgg tggcccgggc ggccaatggg gtgatcggca accagggcgc gatgccctgg
    11281 cacctgccgg cagacctccg gcatttcaag gcgctgacca tgggcaagcc gatgatcatg
    11341 ggccgcaaga ccttcgcctc cctccccggc ctgctgcccg gccggcgcca tatcgtgtta
    11401 acgcgcgatg ccggctgggc cgcgcccggg gcagaaaccg cagccgatgt ggcaggcgcg
    11461 ctggcgctgg ccggcgatgt gccggaaatc tgcgtggtgg gcggcggtga ggtctatgcg
    11521 cagttcctgc cgctggccga tcgcatcgag atcacccata tcgatgccga gcctgccggc
    11581 gacacccatt ttcccgcgct ggccggcccg ccctggcacg aggccgcccg caccgaacac
    11641 ccggcgcaag agggccgccc ggccttcgcc ttcgtcacac tgacccgaag cccataggcc
    11701 acccacaatg atccgcctcg attcgcgcct tccgatcccc gatgccctgc gcggcgccat
    11761 tttcgcgctg ggcaatttcg atggtttcca ccttggccat caggccgttg tcggcgaggc
    11821 gctggcctgg gcccgggccg aggggcggcc ggccatcgtc gccaccttcg atccgcatcc
    11881 ggtcagcgtt ttcgtgcccg atgcgccgcc tttccgcctc accacgcttg atcagcgcga
    11941 ggaactgttt ggcgcggccg gcgccgatgc catgctcgtg ttccatttcg atgcggccat
    12001 ggcggcaacg ccggccgatg gctgggtggc cgatatcctg ggccggcagc tgggcgcggc
    12061 gggcgttgtc accggcgagg atttcacctt cggcaagggg cgcggcggca atgtcgggct
    12121 gctggccgaa atgggcgcgg cccatggcat tggcgcgcgg gcggtgggcc cggtgcgcga
    12181 tgccggcggc gtcatctcct ccagccgcat ccgcgaggcg atcgcggccg gcgacatggc
    12241 cgcggccgcc cgcctgctgt cgcgcccctt tgccattcgc ggccatgtgc agcatggcga
    12301 caaggtgggc cgcaccatcg gctttcccac cgccaacctc gacatggccg attatctgcg
    12361 gccggcctat ggcatctatg cggtgaccgg ccgcctgccc gacgggcggg tgctgaaggg
    12421 cgccgccaat ctgggcatcc gccccacctt caccccgccc aaggaattgc tggagccgca
    12481 tttcttcgat ttttccggcg atctgtatgg ccagcagatc gaggtggcct tccatgcctt
    12541 cctgcgcggc gaggcgaagt tcggctcgct tgatgcgctg atgcagcaga tcgggcgcga
    12601 ttgcgagaag gcgcgcgaga tcctcgaaag catctgaccg cgccttaccg gtttgcagga
    12661 atccctcgca agcgctaagg cacgcgccat catgaccgag aagcgcgact accgagacac
    12721 cgtcttcctg ccgaagaccg atttccccat gaaggcgggc ctcccccaga aggagcccgg
    12781 cattctggcg cgctggcagc agcagggcct ttatcgcgcc ctgcgcgagg cccgcgcggg
    12841 cgccgagaag ttcatccttc acgatggccc cccctatgcc aatggcaaca tgcatatcgg
    12901 ccatgcggtg aaccatatcc tgaaggacat ggtggtgcgc agccagagcc tgctgggcaa
    12961 ggacgcgccc tatgtgcccg gctgggattg ccacgggctt cccatcgagt ggaaggtcga
    13021 ggaagaatac cgcaagaaga agaagaacaa ggacgaggtt ccgccgaagg aattccgcgc
    13081 cgaatgccgc gcctatgcgc agacctgggt gaacgcccag cgcgagcagc tgaagcgcct
    13141 tggcatcaat ggcgactggg acaaccccta tctcaccatg gatttccagg cggaagcaac
    13201 catcgttggc gagctgttga agtttgcgac cagcggccag ctttatcgcg gcgccaagcc
    13261 ggtgatgtgg agcccggtgg aaaaaaccgc gctggccgag gccgaggtgg aatatgagga
    13321 catcacctcc acccagatcg acgtggcgtt cgagatcgtg gaatcgccga ttgccgaact
    13381 ggtcggcgcc tatgcggtga tctggacgac gacaccctgg acgatcccgg ccaatcaggc
    13441 tttggcctat gggccggagg tggattatca cctctatcgc gccgacgatg gccgccgctt
    13501 cctcatcgct gtcgatctgc tgggccattt cctggcgcgc accggccttg gcccccaccc
    13561 cgaagggctg accggcggcc tcgacctggc cgaacatgcg ctggacacca tcaaaggcgc
    13621 cgatcttgcc ggcaccattg cccgccaccc gatggcggcc gccttccccg acagcgcctt
    13681 cttcacccgc ccccgcccct tcctcgccgg cgatttcgtc actaccgaat cgggcaccgg
    13741 ccttgtccac atggcgcccg atcatggcga ggacgacttt gccctgtgca aggcgcatgg
    13801 catcgagccg gtgttcgccg tggaaggcga tggcaaatat cgcgccgact ggccctggct
    13861 gggcggcgag ggcagcgtta tcaaccccaa gttcaacgcg cccgatggcc ccatctgcac
    13921 ggccttgcgc gaagccggca gcttgctggc ggccagcgcc gactacaagc attcctatcc
    13981 gcacagctgg cgttccaagg ccaaggtgat cttccgctgc acgccgcagt ggtttgtgcc
    14041 gatggacaag ccgatcgatc gccagatccc gctttgcgcc accgaagcgg ccatccgcac
    14101 agagcagcac gccggcgaaa ccctgcgcga gatcgccgtg gccgccattg ccgccacccg
    14161 cttcgtgccg gaaaaggggc gcaaccgcat cggcgccatg gtggaaggcc ggcccgactg
    14221 ggtgctttcg cgccagcgcg cctggggcgt gccgatcacc ctgttcgtcg accgcaagac
    14281 gggccagtat ctggctgatc ctgccgttaa cgcccgcatc atcgccgcca tccgcgaaga
    14341 gggcgtggac gcctgggatg aggcgcgcgc gcaggaattt ctcggcgccg attataacgc
    14401 cgcggattac gagcgcgtca ccgacattct cgatgtgtgg ttcgattccg gctccaccca
    14461 tgccttcgtg ctggaaagcg ggcgctggcc cgatcttacg cgccccgccg gccatcaggg
    14521 cccctgggcc gatctgtatc ttgaaggctc cgaccagcat cgcggctggt tccagtcctc
    14581 gctgctggaa agctgcgcca cgcgcggcca tgccccctac aaggccatcc tcacccacgg
    14641 cttcaccatg gatgccaagg ggctgaagat gtcgaagagc ctgggcaaca cggtggatcc
    14701 gctgaaggtg atggaaacct atggcgcgga tatcatccgc ctgtgggcgc tttcggtgga
    14761 ttacaccgag gaccaccgga ttggcgacga gatcctgaag ggcgtggccg accagtatcg
    14821 caagctgcgc aacaccttcc gctatctgct gggcgcgctg gatggctttg ccgaagaaga
    14881 gcgcctgccg gtggccgaaa tgcccgagct ggagcggtat gtgctggcgc tgctgggcgg
    14941 gctggatgcc acgctgaagc aggcgatcgc tgatttcgac ttcaacgcct atgtccgcgc
    15001 gctggtcgat ttctgcaacg aggacctgtc ggccttcttc ttcgatatcc gcaaggatgt
    15061 gctctattgc gatgcgcccg ccagcctgaa gcgccgcgcc tatcgcaccg tgctggacat
    15121 cctgttccac gcgctgatcc gctatgcggc gccggtgctg gtgtttaccg ccgaggaagt
    15181 gtgggccgcc cgctatcccg gggcgcaaag cgtgcatctg ctggagtggc ccgacgtgcc
    15241 ggcggtggac gtggcggcgg aacgctgggc cgcgctgcgg gccctgcgcg ccgaagtgct
    15301 gggcgcgatc gagccgctgc gccgcgacaa gacgctggga tccagcctgg aggccgaagt
    15361 gaccgtgccg gccgatgcgc ccgagggcga tctggccgag ctgttcatct cggccagcgt
    15421 ggcgcgcggc gaggggctgg ccgttgcccg caccgaggca cacaaatgcg gccgctgctg
    15481 gcgccacctg cccgaggtgt ccgaggatgg cgatctgtgc ggccgctgcg aggaaactgt
    15541 cgcgctggtg gatgccggcc ggtgaaaggc gcgcgcgcaa ggcttttggg gctggcggtg
    15601 gcgggccttg tcttccttgc cgatcaggcg gtgaaggcgg ccgtgaccgg cccgctggcc
    15661 ctgcccgaga tcggccagat ccgcctgctg cccatcttca acctgacgtg gacgcagaat
    15721 ttcggtgtct cgctggggct gctcacggcc agttcggatc agcagcgctg gctcctggtg
    15781 gggatcaccg cggccatcgc gctgttcgtc tgcgcctggc tgtggcgcga gaagcgcggg
    15841 ctggatgcgg gcgcgctggg cctcattctg ggcggcgcgc tgggcaacat ccgcgaccgc
    15901 atcaccgccg gtttcgtgat cgattatgcc gacctccatt tcggtgattt ccgccctttt
    15961 ctggtgttta atctggccga tgcggcaatc tcgctgggcg tgctgattat ccttgcccga
    16021 tccttccttt cgcgcgagaa gcgcggggcc gaaacggccc acagcgccac ggagatgtga
    16081 acagcaatgc gcaagatgaa tgccctcgtt ctggcgggcg ccaccacggt cctgctttcg
    16141 ggctgcagtg tcggcaatct tttcaaccgt gtgcgcccgg atgagttcgc cgtgcagcgc
    16201 caggcgccgc tgatggtgcc gccggattat tcgctggccc cgccgcagcc gggtgccccg
    16261 cgccccgcca cccgcaccgc cgagcaggaa acgctggaag ccatgtttgg cggcccggcc
    16321 ccgcgcagcg gcgtggaaaa gaccgtggat ggcaaggccg gcactgccga gcccagcatc
    16381 cgctccaccg tggccgaccc caagaccgcc accgccaaca agggcgatgt gaccaaggac
    16441 atcctcgccg cgccccaggg cgatggccgc gaggcgcagg cccgcatcaa cggcagctga
    16501 tcgccgcgcc agaagcgcat acaaaacccc ccgccagccc caaggccggc gggggttttt
    16561 gtatgcgctt ctggcagatt tatgcggtgc ccttcagcgg gctgaccagc agcttgtcga
    16621 tcttgcgccc gtccatgtcg acgatctcga agcgccagtc ctgatcggca aagctgtcgc
    16681 cctcatcggg caggcggcgc agcaccgaga gcgcatagcc ggcagcggtg gcataatcgc
    16741 ggctgcccgg cagatcgaga cccagccggc cggccagcgc atcggccggc atgctgcccg
    16801 aaatcagcag cgaaccatcg ccgcgctcca ccagcatggg cgcatcgccc tcgtcgcggt
    16861 cgctggcaaa atcgccgaga atggcggtca acaggtcggt gggggtaacg atgccttcca
    16921 aatggccgta ttcgtcatgc accagcgcca tcgccacttc cgattgctgc aggatgcgca
    16981 gcgcgtccat cgcgtccagc tggtcgggca cgatctcggc cttcttcatc agcgcgccaa
    17041 tatcgggggc atcgccctgc agcagcgcgg cctgcacctc gcgcgctttc accacgccga
    17101 cgatcgtgtc gggcgagcca tcggccacgg ccagcagcga atgggggctt tcggtgatcg
    17161 ccacgcgcag atcctggggg gaggcatcgc gctcgatcca gtcgatctgg ttgcgcgggg
    17221 tcatcagctc gcgcacgggg cgatcggcca ggcgcatgat gccggtcatg atcgcccgct
    17281 cctcctcctc gatcacgccg gagcgggtgg cctcggcgaa catcaggtgg atttcctcgg
    17341 cggtcacctg cggctcgtcg tcgtccttct cgccaaacag ccgcagcagc gcccgcgagg
    17401 agcgatcgag gaaccacacc agcggcgcca tgccgcgcgc catcaccgcc atcaccggtg
    17461 ccgccagccc ggcgatcagc tcggcatggc gcagggccag ctgcttgggc accagttcgc
    17521 cgacgatcag gctgaccagc gtggtgccgc ccagcaccag cacgctggca agccggtctg
    17581 ccagcgtggg cgacaggccc cagccgccga tccgttccgc caccggcgcg cccaggctgc
    17641 tttcggaaaa ggcgcccgtg gccagcgcca ccagcgtgat gccgatctgc acggtggaga
    17701 gcagcttgcc cggatcttcc gccagcgcca gcgccaggcg tgagccgcgg tggccacgat
    17761 cagccgccat gcgcagccgc gccggccgcg ccgagacaat cgccagttct gacatggcaa
    17821 acaggccgtt gagcaggacc aggcccgcaa tcaccagcac gtcgatccag gggaaaggcg
    17881 tcacgcttca ggccctagca gctttgcggg gaaaggcgaa gaggcaatgc gccgcccatg
    17941 aaaacaagct tatgcaccgg caatatggcg cgcgcgcggg cccagccggg ccaccgccgc
    18001 ggcatggatc gccggcgctg aaacgagcac gccaaacgcg cgcggatcgg gtttgttata
    18061 gcccagcgcc tggccgaacg catcggacac cgccgcccct gcctcgcgcg cgatcaggca
    18121 ggccgcgcca atgtcccact catagcccca gcgcagcgtg gccagcagat cggcctcgtc
    18181 ggccgccacc atggcaatgc gcagggcaat ggaattgggc tggctgacag gggtgagatc
    18241 ggcatcttcc gggcccagca gccgcgctgg cacccgcgcc ccggccagat ccgcccgggt
    18301 gctggcgcgc agccgccggc catcgcgcgt ggcgccccgg ccctcctcgg ccagccacaa
    18361 ctcgcccccg cccccattgc cgcgcgccgg cgcatgcagc aggccgatca gcggccgccc
    18421 gcaggagatc aacgccaccg acaccgccca gccatcgcgc ccggcaacaa aatcgcgcgt
    18481 gccatcgatg ggatcgacca gccagatgag atcgccatcg cgctggccgg gcgaatcgac
    18541 cgtctcttcc gacagccagc cggcctgcgg caacagcgcg cccagttccg cccgcaaaaa
    18601 ggcatccacc gccatatccg cctcgcacac cggctggcca tcggatttat accatgtttt
    18661 caggggattt tcgcgcgggc tggcccatgg cccctgcccc ggccacgcct tcagcgccag
    18721 cgcgccggct gccctcacga tctgttccag gcgttcccga tcgatcattt ccccgccgcc
    18781 gacctatcca aaatgcattg ttgcgaatca ctctcacacg caagcctttt caaggcctcc
    18841 gccatggctt atgcggcggg ccacacattc gggcgcttgc ccagccatag cgggatctga
    18901 ccatgaacat ccacgagtat caggcgaaag agctgcttgc caagttcggc gttgccattc
    18961 cggccggcca tgccgcgctg accgtcgagg aagccgttgc cggcgccaag ctgctgcccg
    19021 ggccgctcta tgtcgtcaag tcgcagatcc acgccggcgg ccgcggcaag ggcaagttca
    19081 aggaactgcc cgccgatgcc aagggcggcg tgcgcctggc caagagcctg gaagaggtgg
    19141 aaagccactc gcaggaaatg ctgggcaaca ccctcgtcac catccagacg ggcgaggccg
    19201 gcaagcaggt gaaccgcctc tatgtgaccg atggcgttga catcgctcac gaatactacc
    19261 tctcgctgct ggttgaccgc aagaccggcc gcgtggcgat catcgccagc accgagggcg
    19321 gcatggacat cgaggaggtt gcgcacagca cccccgagaa gatcaccacc atcaccatcg
    19381 accctgcctc gggcttccag ccgcaccacg gccgcgccat cgccttcgcg ctgaagctga
    19441 agggcgacac caacaagcag gcccaggtgc tggccaagca gctgtatgat gccttcgtgg
    19501 cgctggattg cgcgatgctg gaaatcaacc cgctggtgga agacaagtcg ggcgcgctgc
    19561 tggtgctgga caccaagatg agcttcgatt cgaacgcgct ctatcgccac cccgacgtgc
    19621 aggccctgcg cgacgagacc gaggaagacc cggccgagat cgaagccagc aagtatgacc
    19681 tcgcctacat caagctggat ggcaacatcg gctgcatggt gaacggcgcg ggcctcgcca
    19741 tggcgacgat ggacatcatc aagctgaacg gcgccttccc ggccaacttc ctcgacgtgg
    19801 gcggcggcgc caccaccgag aaggtgacgg cggcgttcaa gatcatcctc tcggatccgg
    19861 cggtggaagg catcctcgtc aacatcttcg gcggcatcat gaagtgcgac gtcatcgccg
    19921 agggcatcat cgccgcggcc aaggacgtga acctgtcggt gccgctggtg gtccgcctcg
    19981 agggcaccaa tgtcgagaag ggcaaggagc tgctcaacaa ttcgggcctg ccgatcgtgg
    20041 ccgccaacga tctgggcgat gccgccaaga agatcgtggc cgaagtggcc aaggtttcgg
    20101 cctgaacatg atgcgcccgg cgggccttgg cctgccggcg ccctgctgca ggggcctttg
    20161 ccggcctctc agttatgaca ggccccgcag cgcagccgcg ttgcggggct tgacgttatc
    20221 tgcaggccgg gcgattgcaa aaaggcgagt cgtttaatcg atcgactaaa aactgttttg
    20281 gtgaggaaag gccacctgcc atgaagatac tggtgccggt gaagcgtgtg atagactaca
    20341 acgtgaagcc gcgagtgaag gcggacggga cgggtgtgga cctgtcgaac gtgaagatga
    20401 gcatgaaccc gtttgacgag atcgcggttg aggaagggat
//
LOCUS       DASRYE010000248         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_259305,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000248 DASRYE010000000
VERSION     DASRYE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259305"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFF98_12650"
     CDS             <1..514
                     /locus_tag="VFF98_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZU64525.1"
                     /translation="LDLRVIATASAPLEEAARTGAFPPALLYRLSGMRLALPPLRERR
                     EDAESLFVHLLDGAAARLRRPQPAITPAMRAHLAGHDWPGNVRELAQYAERVVLGLEG
                     EAAPGEGALSLAEQVARFEAALLRDAFLAEKGDVNRTATRLAIPRETFYYKAKRLGLD
                     LAGLRRVLAP"
     gene            540..1133
                     /locus_tag="VFF98_12655"
     CDS             540..1133
                     /locus_tag="VFF98_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64526.1"
                     /translation="MTIFRNITELGKGIAARHGWPAAKAFKAYAMTEADRAALSHCAI
                     ELLSLFPPVAGASVLMSAALAVMLERRMQAPVQVVAGTLAVDGAPVFGNRQPLDGAAL
                     FADANPAWDGHAWVMVGPYVADVALFRAAYAAWGPARLARHVDLVFGPNKGLYVDRWA
                     HTGRQGLSYEPQYVLGTEEVTRQMGAAFHLIKAARGA"
     gene            1197..>2476
                     /locus_tag="VFF98_12660"
     CDS             1197..>2476
                     /locus_tag="VFF98_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZU64527.1"
                     /translation="MPPPAITDRFARHFLRGLFDAAIDSADPARAVPAHLPDRPRGRC
                     VVVGAGKASAAMAAAVDAAWPDVALSGVVVTRDGYAVPAGRIEILKAAHPVPDARGVE
                     AARRMLAAVRGLGPDDLVLALVSGGGSALLVAPAPGLTLADKQAVNRALLHSGATIEE
                     MNAVRKHLSAIKGGRLAAAAFPARVVSLLVSDVPGDDPGAIASGPTVADGSTLADARA
                     IVARHAICLPPAARQALERGEETPKPGSVAEDIRIIATPALALRHAAQVARQAGICPL
                     ILGDAIEGEAREAGIVLAGMAQGVRRQGFPLAAPAVLLSGGETSVTIGAGPVGKGGRN
                     SELQLAMALALKGAPGIWSIAGDTDGIDGSEEAAGALVCPDTLARGHALGLDAHRLLA
                     GHDSYSFFRPLGDLVITGPTRTNVNDFRAMLIGGG"
BASE COUNT          303 a          849 c          935 g          389 t
ORIGIN      
        1 gctggatctg cgggtgatcg ccacggcctc cgccccgctg gaggaggccg cccgcacggg
       61 ggccttcccg ccggcgctgc tctatcgcct gtcgggcatg cggctggcgc tgccgccctt
      121 gcgggaacgg cgcgaggatg ccgaaagcct gttcgttcac ctgctggatg gcgcggcggc
      181 ccgcctgcgc cgcccgcagc cggccatcac cccggccatg cgcgcgcatc tcgccgggca
      241 cgactggccc ggcaatgtgc gcgagctggc gcaatatgcc gaaagggtgg tgctggggct
      301 ggagggcgag gcggcccctg gcgaaggcgc cctgtcgctg gccgagcagg tggcccgctt
      361 cgaggcggcg ctgctgcgcg atgctttcct ggccgagaag ggcgatgtga accgcaccgc
      421 tacccgcctc gccatcccgc gggagacgtt ctactacaag gccaagcggc ttggccttga
      481 tctggcgggc ctgcgcaggg tgctggcgcc ctgagccgca aggccctaag gggccgggca
      541 tgacgatttt ccgcaacatc accgaactgg gcaagggcat tgccgcgcgc catggctggc
      601 ccgccgccaa ggccttcaag gcctatgcca tgaccgaggc ggaccgtgcc gccctctcgc
      661 actgcgcgat cgagctgctg tcgctgtttc cgcccgtggc gggcgccagc gtgctgatga
      721 gcgcggccct ggccgtgatg ctggagcggc ggatgcaggc gccggtgcag gtggtggccg
      781 gcacgctggc ggtggatggc gcgccggtgt tcggcaaccg ccagccgctg gatggcgccg
      841 cgctgtttgc cgatgccaac cctgcctggg atggccatgc ctgggtgatg gtgggcccct
      901 atgtggccga tgtggcgctg tttcgcgcgg cctatgcggc ctggggcccg gcgcggctgg
      961 cgcggcatgt cgatctcgtc tttggcccca acaaggggct ctatgtcgat cgctgggcgc
     1021 acaccggccg gcagggcctg tcctacgagc cgcaatatgt gctgggcacg gaggaagtga
     1081 cgcggcagat gggcgcggcc ttccacctca tcaaggctgc ccggggggcc tgaacgcgcc
     1141 gccgatcggt gctatcctgc ttgctgcccg cgcgacagat ggaaccgatg cccgccatgc
     1201 ccccgcctgc catcaccgac cgttttgccc gccatttcct gcgcggcctg ttcgatgccg
     1261 cgatagacag cgccgatccc gcgcgggcgg tgcctgccca tctgccagac aggccgcgcg
     1321 ggcgctgcgt ggtggtgggc gcgggcaagg cctctgccgc catggccgca gcggtcgatg
     1381 ccgcctggcc ggatgtggcg ctttccggcg tggtggtcac ccgggatggc tatgccgtgc
     1441 cggccgggcg gatcgagatc ctcaaggccg cccacccggt gcccgacgcg cgtggcgtgg
     1501 aagcggcgcg gcgcatgctg gccgccgtgc gcgggctggg gcccgatgat ctggtgctgg
     1561 cgctggtgtc gggcggcggc tcggcgttgc tggtggcccc ggcgccgggc ctgacgctgg
     1621 ccgacaagca ggcagtcaac cgggcgttgc tgcacagcgg cgccacgatc gaggagatga
     1681 atgccgtgcg caagcatctc tccgccatca agggcgggcg gctggcggcg gcggcgtttc
     1741 cggcgcgggt ggtctcgctg ctggtatcgg atgttccggg cgatgatccc ggcgccattg
     1801 cctctggccc caccgtggcc gatggcagca cgctggccga tgcccgcgcg attgtggcgc
     1861 gccatgccat ctgcctgccg cccgctgccc ggcaggcgct ggagcggggc gaggaaaccc
     1921 ccaagcccgg ctctgtggcc gaggatatcc gcatcatcgc cactcccgcg ctggcgctgc
     1981 gccatgccgc gcaagtggcc cggcaggcgg gcatctgccc cctgatcctg ggcgatgcca
     2041 tcgaggggga agcgcgcgag gcgggcattg tgctggccgg catggcgcaa ggggtgcgcc
     2101 ggcaggggtt tccactggcc gcgcccgccg tgctgctctc gggcggcgag acgagtgtga
     2161 ccattggcgc cggccctgtc ggcaagggcg ggcgcaacag cgaattgcaa ctggcgatgg
     2221 cgctggcgct gaagggggcg ccgggcatct ggtcgatcgc cggcgacacc gatggcatcg
     2281 atggcagcga ggaggccgcc ggcgctctcg tctgccccga tacgctggcg cggggccatg
     2341 cgctggggct ggatgcccat cgcctgctgg ccgggcatga cagctacagc ttcttccggc
     2401 cgctgggcga tctggtgatc accggcccca cgcgcaccaa tgtcaatgat ttccgcgcca
     2461 tgctgatcgg cggggg
//
LOCUS       DASRYE010000249         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_29305,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000249 DASRYE010000000
VERSION     DASRYE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29305"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFF98_12665"
     CDS             <1..1462
                     /locus_tag="VFF98_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZU64528.1"
                     /translation="APLPPPPPADAPGADTSGGDALPPPPSPPPPPLLAPPALLPPAS
                     PDMLADPGYLVDRARFLLRRGMGLQAAQMLAGRPPLARPAFDPRRWVGVSLAAARAAA
                     TALDAGDAALVATGAVEGFVPGTDIARQSFAIRDDYTSLMWLGGTSALWQLHDSARAA
                     LLFARYATAARTPQTRAKGFYWAGRALAAQRDTPGARGYFGSAAVYADQYYGQLALER
                     LGLPMPPMADTAHPLPTDSQRAAFQARPITRAVREVARDADWQTTIKFFREIANQAQD
                     ESDFVLLGDYARTLGRRDLAVVAGQAAENAGYQNFRSLCFPLMPLPATADFTMVHAIT
                     RQESQFSQNALSRTGARGLMQLMPSTAEGVARHLGLAYSAGALTQDASFNTALGGAYF
                     AHLLEVFSGSYPLAIAAYNAGPGAVRRWLGQYGDPRSTGADWVEWIEKLPYGETRGYV
                     QHVIENAVVYEAMNPARASYRGPNPTSHFLGKGGPG"
     gene            1462..2013
                     /gene="greB"
                     /locus_tag="VFF98_12670"
     CDS             1462..2013
                     /gene="greB"
                     /locus_tag="VFF98_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZU64529.1"
                     /translation="MVFGSGRLRTAKGPRPAAPPKAPGEGPPITPAGFAALRARYDHL
                     LGTERPQIVEIVSWAAGNGDRSENGDYLYGRKRMREIDRELAFLARRMKAVRVVDPAR
                     QEERTRVWFGASVVIADEDDNRRALTLVGDDEQDAGKGLIGWSAPIARALRGARLGDL
                     RLVRLPGGEKEWEVMEITYPDPA"
     gene            complement(2142..>3044)
                     /locus_tag="VFF98_12675"
     CDS             complement(2142..>3044)
                     /locus_tag="VFF98_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64530.1"
                     /translation="NDTKSTRLLKASGTYAYAPYVRLSDTKPSWDASLTYALVPEASL
                     YARVARGFRGPTIQGRSAVFGSNWTTAGSETNTSWEAGVKTSWLGNRVHFNLDGFYYT
                     VKNIQLNGYDSNNNAVLFNANKAVGYGLEAELDAHPTSRLKLNAGLSLLHTEIKDPNV
                     YVQACSLSGALVCTLQNPYYAKGTSYYAQINGNALPNAPKFNLNLGARYDLPLGPGKA
                     YLSTDWNFQGRTYLTAYKAVEFSTNGNMEGGLKGGYNWGMYDVSVWVRNITNRHNLIG
                     VLDSYMAGAYNDPRTIGVAGSMHF"
BASE COUNT          460 a         1090 c         1006 g          488 t
ORIGIN      
        1 tgcccccctg cccccgccgc cccctgccga tgcccccggc gccgacacat ccggcggcga
       61 tgcgctgccg cccccgccat caccgccccc cccgccgctg ctggcacccc cggccctgct
      121 gccccccgcc agccccgaca tgctggcaga cccgggctat cttgttgacc gcgcccgctt
      181 cctgctgcgc cggggcatgg gcctgcaggc cgcgcagatg ctggccggcc gcccgccgct
      241 ggcccgcccc gccttcgatc cgcgccgctg ggtgggggtc agcctggctg ctgcccgcgc
      301 cgccgccacc gcgctcgatg ccggcgatgc cgcgctggtc gccaccggcg ctgtggaagg
      361 cttcgtgccc ggcaccgata tcgcccggca aagcttcgcc atccgcgacg attacacctc
      421 gctgatgtgg ctgggcggca cctcggccct gtggcaattg cacgacagcg cccgcgccgc
      481 gctgctgttc gcccgctatg ccaccgccgc ccgcaccccg cagacccgcg ccaagggctt
      541 ctactgggcc ggccgcgccc ttgccgcgca gcgcgacacg ccgggcgcgc gcggctattt
      601 cggcagcgcc gccgtctatg ccgaccagta ttacggccag cttgcgctcg aacgcctggg
      661 cctgccgatg ccgcccatgg ccgacaccgc ccaccccctg cccaccgaca gccagcgcgc
      721 cgctttccag gcgcggccga tcacccgcgc cgtgcgcgaa gtggcccgcg atgccgactg
      781 gcagacgacc atcaaattct tccgcgaaat cgccaatcag gcgcaggacg agagcgattt
      841 cgtgctgctg ggcgattatg cccgcacgct gggccggcgc gatctggccg tggtggccgg
      901 ccaggcggcg gaaaacgcgg gctatcagaa tttccgcagc ctgtgcttcc cgctgatgcc
      961 gctgccggcc acggccgatt tcaccatggt ccacgccatc acccggcagg aaagccagtt
     1021 cagccagaac gccctttcgc gcaccggcgc gcgcgggctg atgcagctga tgccatctac
     1081 ggccgaaggg gtagcgcgcc atctcgggct ggcctattcg gccggcgccc tcacgcaaga
     1141 tgccagcttc aacacagcgc tgggcggggc ctatttcgcc catctgctgg aggtgttctc
     1201 cggcagctat ccgctcgcca tcgccgccta caacgccggg ccgggcgcgg tgcgccgctg
     1261 gctgggccaa tatggcgatc cgcgcagcac cggcgccgac tgggtggaat ggatcgagaa
     1321 gctgccctat ggcgaaaccc gcggatatgt gcagcatgtg atcgagaacg ccgtggtcta
     1381 tgaagcgatg aaccccgccc gcgccagcta tcgcgggccc aatcccacca gccatttcct
     1441 cggcaagggg gggccgggct gatggtgttc ggttccggcc gcctgcgcac cgccaagggc
     1501 ccgcgccccg ctgccccgcc caaggccccc ggcgaggggc cgccgatcac gccggccggc
     1561 tttgcggcct tacgcgcccg ctatgatcat ctgctgggca ccgagcggcc gcagatcgtc
     1621 gagatcgtca gctgggcggc cggcaatggc gacaggagcg agaatggcga ctatctctat
     1681 ggccgcaagc gcatgcgcga gatcgaccgc gagctggcct tccttgcccg gcgcatgaag
     1741 gcggtgcggg tggtcgatcc ggcccggcag gaggaacgca cgcgcgtgtg gttcggcgcc
     1801 agcgttgtca tcgccgacga ggacgacaac cgccgcgccc tcacccttgt cggcgatgac
     1861 gagcaggatg ccggcaaggg cctgatcggc tggagcgccc ccatcgcccg ggccctgcgc
     1921 ggcgcgcggc tgggcgatct gaggctggtg cgcctgcccg gcggcgagaa ggaatgggaa
     1981 gtgatggaga tcacctatcc ggatccggcc tgatcccgat ccggcctgac cgccacacaa
     2041 gaaaggggcc ggccccgcaa gcaaggccag cccctttccc cctgttgccc tgagcgcggg
     2101 gggaacgctg caacagcgcc ccccgcacgg gacgaaacgg atcagaagtg catcgatccg
     2161 gcaacgccga tggtgcgcgg atcattgtag gcgccggcca tgtagctatc gagcacgccg
     2221 atcagattgt gccggttggt gatgttgcgc acccacaccg acacatcata catgccccag
     2281 ttgtagccgc ccttcaggcc gccttccata ttgccgttgg tggagaattc cacggccttg
     2341 taggcggtga gataggtgcg gccctggaag ttccagtcgg tcgacagata ggccttgccc
     2401 ggccccagcg gcagatcata gcgcgcgcca aggttcaggt tgaacttggg cgcgttgggc
     2461 agggcattgc cgttgatctg ggcataatag ctggtgccct tggcgtaata ggggttctgc
     2521 agcgtgcaca ccagcgcccc gctcagcgag caggcctgca catagacgtt gggatccttg
     2581 atctcggtgt gcagcagcga gaggcccgcg ttcagcttca ggcggctggt cggatgggca
     2641 tccagctcgg cttccaggcc atagcccacc gccttgttgg cgttgaacag caccgcgttg
     2701 ttgttgctgt catagccgtt cagctggatg ttcttcaccg tgtagtagaa gccatcgagg
     2761 ttgaagtgca cccggttgcc cagccacgag gtcttcacgc cggcttccca gctggtgttg
     2821 gtctcgctgc cggcggttgt ccagttgctg ccgaacacgg ccgagcggcc ctggatggtg
     2881 gggccacgga agccgcgcgc cacacgggca taaaggctgg cctcgggcac cagcgcatag
     2941 gtgaggctgg catcccagct cggcttggtg tccgacaggc gcacataggg cgcataggca
     3001 taggtgcccg acgccttgag caggcgggtg ctcttggtgt cgtt
//
LOCUS       DASRYE010000250         7294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_121476,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000250 DASRYE010000000
VERSION     DASRYE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121476"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VFF98_12680"
     CDS             complement(<1..1601)
                     /locus_tag="VFF98_12680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZU64531.1"
                     /translation="MIRRLLLCASALLPLPLLPLPALAATGPFASPSTLPFEAPDFTR
                     LHDADYQPAIEQAIALQRAEIEAIAANPAPPSFANTIEAMERSGAMLTRATNVFSALV
                     AANTNDTLDKADTATAPQLAALHDAIMMNAQLFARVNHLYEQRAHLGLAPDQLQLLEV
                     TWRGFRLSGALLAPEAKLQLQAINQQLSTLQTSFSQKLVAATKDAAPTFADKAQLAGL
                     AEGDLAAAAKAAEERKAPAGSYVLPLQNTLQQPALESLTNRATRQALYTASATRAEQG
                     NANDTRATILEIARLRAQKAQLLGYPDYASLALADQMAQTPARAIAFMQALVPPLAAQ
                     AHAEAADLDALIAKEGGHFTVEPWDWPLYAAQLRKSRLAFDAASAKPYFEITRVLEQG
                     VFYAAHQLYGLSFEKRGDIPTYHRDVSVYTVKNAQGQAIGLFYFDPWKRDNKQGGAWM
                     SNFVQQSTLLGEQPVVFNVENFTPPAPGQPALVGFDDVVTMFHEFGHALHGLLSDQRY
                     PSLAGTSTARDFVEFPSQFNENWAT"
     gene            complement(1598..2449)
                     /locus_tag="VFF98_12685"
     CDS             complement(1598..2449)
                     /locus_tag="VFF98_12685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZU64532.1"
                     /translation="MRGSRRAVLAGLAAAGWQAGSGPWTGRAKATSPALPFGPLCQGG
                     WMRGFAPPGAQAVSLDGQPVPLAQGRFFLGFDRDAPPGALLRIDEASFPLAIAPRAWA
                     IEQVNAPFHPPGLPDATFAALRTGELARIHAARARATDAAGWNQPFQRPAEGRFSGFF
                     GAQRIYQGGVAGAYHGGLDIAAAAGTPYRAPAAGVVVLAAEAPFTLEGNLLILDHGMG
                     LNSAFLHASALLVREGEAVAQGQPLGLVGQTGRATGPHLHWAVMWHSARLDPLLLLPA
                     PLREGHP"
     gene            complement(2458..2655)
                     /locus_tag="VFF98_12690"
     CDS             complement(2458..2655)
                     /locus_tag="VFF98_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HZU64533.1"
                     /translation="MLTPSAKPAMLLYEPSSFRIISPGNHVICAVSGEQIPLEALRYW
                     SVLHQEAYASCELATRRLTGH"
     gene            complement(2682..4142)
                     /gene="xseA"
                     /locus_tag="VFF98_12695"
     CDS             complement(2682..4142)
                     /gene="xseA"
                     /locus_tag="VFF98_12695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZU64534.1"
                     /translation="MAASSFSDDGISDALVARDAPGDNAAPLSISEISAALKRVVEDR
                     FGFVRVRGELSGVKRAASGHLYLALKDENARLDGVMWKGNAARLGFRPEDGVEVIATG
                     KLTTYPGRSNYQLVIERMEVAGEGALLALLARLKARLEAEGLFDPRRKRALPFLPQVI
                     GVVTSPTGAVIRDILHRLADRFPSHVLLWPVLVQGQGAAEQVAAAIRGFSALDGTGPV
                     PRPDVLIVGRGGGSIEDLWAFNEEVVVRAIADCTIPVISAVGHETDTTLADFAADRRA
                     PTPTAAAEMAVPVRAELAAQLDELAYRQRRAIQRALTLGRERLAARADRLPAPAALLE
                     GPTQTLDGAAEALKRGLSQRIVVAGTALQRQAGRLSLPLLAARLERARDRLAARRLAP
                     ALLTQRLAEGQRHLDASARIMASLNPDNVLKRGYARVTDAEGRTLMSREAAAAEAALT
                     LHFRDGRLDVAPAEGAARGPRKAPAKPAAPEQGKLL"
     gene            4141..5436
                     /gene="purD"
                     /locus_tag="VFF98_12700"
     CDS             4141..5436
                     /gene="purD"
                     /locus_tag="VFF98_12700"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZU64535.1"
                     /translation="MNILLLGSGGREHALAWKLAQSSLLAGGGKLYAAPGNPGIAGHA
                     TCVALDVADHGAVIAFCDAHAIGLVVVGPEAPLVDGLGDSLKGAGVPVFGPSALAARL
                     EGSKGFTKDLCTRADIPTAGYARVASAEAAAAALARFGAPVVIKADGLAAGKGVTVAM
                     TMAEAEEAVAEIFAGRFGAGAEAVIEEFMEGEEASFFALTDGATIQPFASAQDHKRVG
                     DGDTGPNTGGMGAYSPARVLTPELEAEVMTRIIAPTVKTLADEGMPYVGVLFAGLMLT
                     AEGPKLIEYNARFGDPECQVMLSRLESDLGELLLAAATNRLGSVAPPRFSPDVALTVV
                     MAANGYPGTPEKGSPIGALAEAEAGGDVRVFHAGTALVDGQLVASGGRVLNVTARGAT
                     VSAAQAAAYRAVDAIAAPGLFCRRDIGWREVAREEAGEG"
     gene            complement(5447..5647)
                     /locus_tag="VFF98_12705"
     CDS             complement(5447..5647)
                     /locus_tag="VFF98_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64536.1"
                     /translation="MAGGVSPISPVTASGPQSKYVYAATAVSPSVPKASDFGDNRPKG
                     LAPIVDVSPTPPKDPTNINIMA"
     gene            complement(5686..5892)
                     /locus_tag="VFF98_12710"
     CDS             complement(5686..5892)
                     /locus_tag="VFF98_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64537.1"
                     /translation="MSSISPAGAQGAAAAYQQTATRAAQGPVDADGDHDGTSAAKAPA
                     AKTAPAPAAAPQPKSPGATVTVYA"
     gene            complement(6003..6998)
                     /locus_tag="VFF98_12715"
     CDS             complement(6003..6998)
                     /locus_tag="VFF98_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HZU64538.1"
                     /translation="MSEPRIDVIAIGNAIVDVMAPASEEDIAALGLAKGGMTLVDPAR
                     AQELYAAMGPAREISGGSAANTLAGLAELGAACAFIGQVADDQFGRVFAHDIRAGGIA
                     FATPVREGEPATAQCLIFVTPDGQRTMNTFLGASQFLPATALDEATIASARVLYLEGY
                     LWDPEEPRKAMRRAIAAARAAGREVAFTLSDGFVIARHGDDFRQLIEEGQIDILFANE
                     HELAALTGIEDFHEGLASLHGRVPTVVVTRSAQGAHAFRGDEHAQVPAAPVTHVVDTT
                     GAGDLFAAGFLFGHVRGLALDACLKLGALCAAEIISHYGARPEADLKALVAPLLA"
BASE COUNT         1063 a         2371 c         2748 g         1112 t
ORIGIN      
        1 tcggtggccc agttctcgtt gaactggctg ggaaattcca cgaaatcgcg cgccgtgctg
       61 gtgcccgcca ggctgggata gcgctggtct gacagcaggc cgtgcagggc atggccaaat
      121 tcgtggaaca tcgtcaccac atcgtcgaag ccaacgaggg cgggctggcc gggggcgggc
      181 ggggtgaaat tctccacgtt gaagaccacc ggttgttcgc ccagcagggt ggattgctgg
      241 acgaaattgc tcatccacgc gccgccctgc ttgttgtcgc gcttccacgg gtcgaaatag
      301 aacaggccga tcgcctgccc ctgcgcgttt ttcacggtgt agacgctgac atcgcggtga
      361 taggtgggga tatcgccgcg cttctcgaag gagaggccat agagctggtg ggcggcatag
      421 aacacgccct gttccagcac gcgggtgatc tcgaaatagg gcttggcgct ggcggcatcg
      481 aaagcgaggc ggcttttgcg caattgcgcg gcatagagcg gccagtccca cggctctacg
      541 gtgaaatggc cgccctcctt ggcgatcagc gcatccagat ccgcggcttc ggcatgggcc
      601 tgggctgcca gcgggggcac cagcgcctgc atgaaggcga tggccctggc cggcgtttgc
      661 gccatctggt cggccagcgc cagcgaggca taatcgggat agcccagcaa ttgcgccttt
      721 tgcgcgcgca gccgggcgat ttccagaatg gtggcgcggg tgtcgttggc attgccctgt
      781 tcggcgcggg tggcgctggc ggtgtagagc gcctgccgcg tggcgcggtt ggtcaggctt
      841 tccagcgcgg gctgctgcag ggtgttctgc aagggcagca catagctgcc ggcgggcgcc
      901 ttgcgttcct cggcggcctt ggcggcagcg gcgaggtcgc cttcggcaag gccggccagc
      961 tgggccttgt cggcaaaggt gggggcggca tccttggtgg ccgccaccag cttttgcgag
     1021 aagctggtct gcagggtgga aagctgctgg ttgatcgcct gcagctgcag cttggcctcg
     1081 ggcgccagca gcgcgccaga caggcgaaag ccgcgccagg tcacttccag cagctgcagc
     1141 tgatcgggcg cgaggccgag atgggcgcgc tgttcgtaga ggtggttcac ccgggcgaac
     1201 agctgcgcgt tcatcatgat cgcatcgtgc agcgctgcca gctgcggggc ggtggcggtg
     1261 tctgccttgt cgagcgtgtc gttggtgttg gcggccacca gggcggaaaa cacgttggtg
     1321 gccctggtca gcatggcgcc cgatcgttcc atcgcctcga tcgtgttggc aaagctgggc
     1381 ggggcggggt tggcggcgat ggcctcgatc tcggcgcgct gaagggcgat ggcctgctcg
     1441 atcgccggct gatagtcggc atcgtgcagc cgggtgaaat cgggcgcctc gaagggcagg
     1501 gtggaagggc tggcgaaagg cccggtggcg gccagcgcgg gcagcggcag caagggcagc
     1561 ggcagcaggg cggaggcgca aagcagcagg cggcggatca tgggtgtcct tcgcgtaagg
     1621 gggcggggag gagaagcagc ggatcgagcc gggcgctgtg ccacatcact gcccagtgca
     1681 ggtgggggcc ggtggcgcgc ccggtctggc cgacaaggcc cagcggctgg ccctgcgcca
     1741 ccgcctcgcc ttcgcgcacc aagagggccg aggcgtggag gaaggcgctg ttgaggccca
     1801 tgccatgatc gaggataagg aggttacctt ccagcgtgaa cggcgcctcg gccgccagca
     1861 cgaccacccc tgcggccggc gcgcgatagg gggtgccggc ggccgcggca atatccagcc
     1921 cgccgtgata ggcgccggcc accccgccct gatagatgcg ctgggcgccg aagaagccgg
     1981 agaagcgccc ctcggccggg cgctggaagg gctggttcca gccggcggca tcggtggcgc
     2041 gggcgcgggc ggcgtggatg cgggccagct cgcccgtgcg cagggcggca aaggtggcat
     2101 cgggcaggcc cggcgggtgg aagggggcgt tcacctgctc gatcgcccag gcgcgcgggg
     2161 cgatggcgag ggggaagctg gcctcgtcta tgcgcagcag ggcgccgggc ggggcatcgc
     2221 ggtcgaaacc gaggaagaag cggccttgcg ccagcggcac cggctgccca tccagcgaga
     2281 cggcctgcgc gcccggcggg gcaaagccgc gcatccagcc gccctggcac agcgggccaa
     2341 agggcagcgc cgggctggtg gcctttgccc tgccggtcca tggcccgctg cccgcctgcc
     2401 atccggccgc ggccaagccg gccagcaccg cccgccggct gccgcgcatg ggcgcgctca
     2461 gtggccggtc aaccggcggg tggccagctc gcaggaggca taggcttcct ggtgcagcac
     2521 gctccagtag cgcagagctt ccagcgggat ctgctcgccc gagacggcgc agatcacatg
     2581 gttgcccggg ctgatgatgc ggaaactgct cggttcgtaa agcagcatgg cgggcttggc
     2641 ggaaggcgtg agcatggcag gggtttatcc ttggctgcgg gtcaaagcaa tttgccctgt
     2701 tcgggggcgg cgggtttggc aggggccttg cgggggccgc gggcggcgcc ttctgccggg
     2761 gccacatcca gccggccatc gcggaaatgg agagtgaggg cggcttcggc ggcggcggcc
     2821 tcgcggctca tcagcgtgcg gccctcggca tcggtcacgc gggcatagcc gcgcttcagc
     2881 acattgtcgg gattgaggct ggccatgatc cgcgccgagg catcgagatg gcgctgcccc
     2941 tcggccagcc gctgggtgag gagggcgggc gccaggcgcc gggccgccag ccgatcacgc
     3001 gcgcgctcca gccgggccgc cagcagcggc agcgagaggc gcccggcctg ccgctgcagc
     3061 gccgtgccgg ccacgacaat gcgctgcgag aggccgcgct tcagcgcctc ggccgcgcca
     3121 tccagcgttt gcgttggccc ttccagcagc gctgcggggg cgggcagccg gtcggcgcgg
     3181 gcggccagcc tctcgcgccc cagcgtgagg gcgcgctgga tggcgcggcg ctggcgatag
     3241 gccagctcgt cgagctgggc ggccagttcg gcgcgcacgg gcaccgccat ttcggccgcc
     3301 gcggtgggag tgggggcgcg ccggtcggcg gcgaaatcgg ccagtgtggt gtccgtctca
     3361 tggccaacgg cgctgatcac ggggatcgtg cagtcggcga tggcgcgcac caccacttcc
     3421 tcgttgaagg cccacagatc ctcgatcgag ccgccgcccc ggccaacgat cagcacatcc
     3481 ggccggggca ctggccctgt gccatccagc gcggaaaaac cgcggatggc ggcggccacc
     3541 tgctctgccg cgccctgccc ctgcaccagc accggccaca gcagcacatg gctggggaag
     3601 cggtctgcca ggcggtggag gatatcgcgg atgacagcgc cggtggggct ggtgaccacg
     3661 ccgatcacct gcggcaggaa cggcagcgcc cgcttgcggc gcggatcgaa caggccctct
     3721 gcctccagcc gggccttcag cctggccagc agcgccagca gcgcgccttc gccggccact
     3781 tccatccgct cgatcaccag ctgatagttg gagcggccgg gataggtcgt cagcttgccg
     3841 gtggcgatca cttccacgcc atcttccggg cgaaagccaa ggcgcgcggc attgcccttc
     3901 cacatcacgc catccagccg ggcgttctcg tccttcagcg ccagatagag atggcccgag
     3961 gccgcccgct tcacccccga aagctcgccg cgcacgcgca caaagccgaa gcgatcttcc
     4021 accacgcgct tcagggcggc ggaaatctcg ctgatcgaca ggggtgcggc attgtcgccg
     4081 ggcgcgtccc tcgctaccag cgcgtcggaa ataccatcgt cggaaaaaga agaggcggcc
     4141 atgaatatcc ttctgctggg ctcgggcggg cgcgaacatg cgctggcgtg gaagttggcg
     4201 caatcctcgc tgcttgccgg tggtgggaaa ctttatgcag cgccgggcaa tccgggcatt
     4261 gccggccatg ccacctgcgt ggcgctggat gtggccgacc atggcgccgt gatcgccttt
     4321 tgcgatgccc atgccattgg cctggtggtg gtgggccctg aagcgccgct ggtcgatggg
     4381 ctgggcgatt cgctgaaggg ggcgggcgtg ccggtgttcg gcccttcggc gctggctgcc
     4441 cggctggaag gctccaaggg cttcaccaag gatctgtgca ccagggccga tatccccact
     4501 gccggctatg cccgcgtggc cagcgccgag gcggcggcgg ccgcgctcgc ccggtttggc
     4561 gcgccggtgg tgatcaaggc cgacgggctg gccgccggca agggcgtcac cgtggcgatg
     4621 accatggccg aggctgagga ggctgtggcc gagatctttg ccggccgctt tggcgccggc
     4681 gccgaggcgg tgatcgagga attcatggaa ggcgaggaag ccagcttctt tgccctgacc
     4741 gatggcgcca ccatccagcc ctttgccagc gcgcaggatc acaagcgcgt gggcgatggc
     4801 gacaccggcc ccaacaccgg cggcatgggc gcctattccc ccgcccgcgt gctgacgccc
     4861 gagctggaag ccgaggtgat gacccgcatc atcgccccca cggtgaagac gctggccgat
     4921 gagggcatgc cctatgtggg ggtgctgttc gccgggctga tgctgacggc cgaaggcccg
     4981 aagctgatcg aatataacgc ccgcttcggc gatccggaat gccaggtgat gctcagccgg
     5041 ctggaatcgg atctgggcga attgctgctg gccgcggcca ccaaccggct gggttccgtg
     5101 gcgccgccgc gcttttcgcc cgatgtggcg ctgacggtgg tgatggccgc caatggctat
     5161 cccggcacgc ccgagaaggg cagcccgatc ggcgcgctgg ccgaggccga ggccggcggc
     5221 gatgtgcgcg tgttccacgc cggcacggcg ctggtcgatg gccagctggt ggccagcggc
     5281 gggcgcgtgc tgaacgtgac ggcgcgtggc gccaccgtca gcgccgcgca ggccgccgcc
     5341 tatcgcgcgg tggatgcgat tgcggcgccg ggcctgttct gccggcgcga cattggctgg
     5401 cgcgaagtgg cccgcgagga agccggggag ggctgagcgc cccccctcag gccatgatgt
     5461 tgatgttggt ggggtctttc ggcggggtgg gcgacacatc gacgatcggc gccaggccct
     5521 tgggccggtt gtcgccgaaa tcggaagcct tgggcaccga gggcgacacg gccgtggccg
     5581 catagacata tttcgattgc gggcccgatg ccgttacggg cgagatgggg gaaacgccac
     5641 cggccatggc atggttcctt tccggttggg gggtgaacgg cgcgctcagg cgtagacggt
     5701 gaccgtggcg cccggcgatt tgggctgggg cgctgcggca ggagcagggg cagtcttggc
     5761 agcgggcgcc ttggccgcgc tcgtgccatc atgatcgcca tcggcatcaa cggggccttg
     5821 cgccgcccgc gtggcggttt gctgataggc ggcggcagcg ccttgcgcgc cggcggggga
     5881 aatgctgctc atgagacccg gtgctccgtt ctgtgcggcg gcccatgcca ccacaacgac
     5941 agaacggcag gccggcgccc ggtttaaggg cgccagcagc gatccgggcc gtaatccggt
     6001 cttcaggcga gaagcggggc caccagcgcc ttgaggtcgg cctcgggccg ggcgccataa
     6061 tggctgatga tctcggccgc gcacagggcg cccagcttca ggcaggcatc cagcgccagg
     6121 ccgcgcacat ggccgaacag gaagccggcg gcaaacagat cgccggcgcc ggtggtatcc
     6181 accacatggg tcacgggcgc tgccggcacc tgcgcatgtt cgtcgccgcg gaaggcatgg
     6241 gcgccctgcg cgctgcgggt gacgacgacg gtgggcacgc ggccatggag gctggccagc
     6301 ccttcgtgga aatcctcgat gccggtaagg gcggccagtt catgctcgtt ggcgaagagg
     6361 atgtcgatct ggccctcctc gatcagctgg cggaaatcat cgccatggcg ggcgatgaca
     6421 aagccgtcgg acagggtgaa ggccacttcg cggccggccg cgcgggccgc ggcaatggcc
     6481 cggcgcatgg ccttgcgcgg ctcttccgga tcccacagat agccttcgag atagagcacc
     6541 cgggccgagg cgatggtcgc ctcgtccagc gcggtggccg gcaggaactg gctggcgcca
     6601 aggaaggtgt tcatcgtgcg ctggccatcg ggggtgacga agatcaggca ctgggcggtg
     6661 gccggctcgc cttcgcgcac gggggtggca aaagcgatgc cgccggcgcg gatatcatgc
     6721 gcgaagacgc ggccgaactg gtcgtcggcc acctggccga tgaaggcgca ggccgcgccc
     6781 agctcggcca ggcccgccag cgtgttggcg gccgatccgc ccgagatctc gcgcgcgggg
     6841 cccatcgcgg catagagttc ctgcgcgcgg gccggatcca ccagcgtcat gccgcccttg
     6901 gccaggccca gcgccgcgat atcctcttcg ctggccggcg ccatcacatc gacaatggca
     6961 ttcccgatgg cgatcacatc gatgcggggc tctgacacgg ggtttcgttc tttcctgcag
     7021 gaggggctgt ggttgcggcg caccgcctag ccgccggccg ggatcagggc aaggaaaagg
     7081 gcgcaggccg cgctctgcct ggcgcaagtg gcgcgccggc gtggttgaca taggcgatgg
     7141 ccccggcaat cacccgcgcc atgtttgccg ccctgcgccc tgccccgcct gccgcctttg
     7201 gccgcctgct gcccttcctg ccggcgctgg cgctgctggc gggttgcggt ggcggcaccc
     7261 ctgtggccag cccggggtcg cgccccgcgc cgcc
//
LOCUS       DASRYE010000251         8307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_15615,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000251 DASRYE010000000
VERSION     DASRYE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15615"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..439)
                     /locus_tag="VFF98_12720"
     CDS             complement(140..439)
                     /locus_tag="VFF98_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64539.1"
                     /translation="MRNWTNGAPRAYERGSNKGAGALLLADKDWNNRVTKAIKLTLTE
                     DEAEMIVDALEADMEGYLESAKEARGNANREDVKTFGEAAERIAGLLGKVRALLD"
     gene            complement(449..1438)
                     /locus_tag="VFF98_12725"
     CDS             complement(449..1438)
                     /locus_tag="VFF98_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU64540.1"
                     /translation="MDVARVAGVSKSAVSRAFTGGIVSEDARRKIMEAARLLRYRPSN
                     SARSLTTNRSHLIGLAITSLDNLFYPLMVERLHHKLAAAGYRLVLFITHGDADLDPVL
                     DELLAYRLDGVILASSSRAARVARECLEGGVPVVMLNNVDPEGRIPGVCTDAEAGTRA
                     IAGHLLASGRRRIGLVSGLEESSTSVERSAAFTRAIAEHPEACLFTACGQFSAEGAYA
                     AARKLLSLRQPVEALFCVTDFMALSALEAVRDMGLEPGCEVAVFGFDDVPVGRRPAFA
                     LATYAQPLEAMVDATLEMLYAQIEGQPLADGIRRLEGQLVLRQSGGQIPPAGG"
     gene            1836..3071
                     /locus_tag="VFF98_12730"
     CDS             1836..3071
                     /locus_tag="VFF98_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64541.1"
                     /translation="MTKHHHPGIMTGVALLLPITLSTMAIVLLAPVLPKILEEFAAVP
                     GHDYWVPMILTIPALCIALFSTLAGALGDFFGRRRLLIASFCVYAVVGIAPLFLHDLT
                     AILVSRVGVGLAEALIMVLSTTMIGDYFHGAARDKWLAAQTAFASMSALVFFNVGGQL
                     GAFGWRTPFWVYLSALVMLVLVLAFTWEPQGDTHDDDISDEAEAAPHNASWAGFPWAR
                     MALILAITIYGAVFFYTVQIQAAGGLALLGVNSPAQAGFLTSIASIGVPLGTFIYSRV
                     GRAPVQRLLMAEFALLAVGFTVMGRAPSPHLFVLGCFINQLGAGLLLPTLLVWAMSLL
                     AFEIRGRGAGMWQSAFALGQFLSPVVVTSAALRTGGLQNAFTVLAAGALLGLVVVTLA
                     GRRIRRSDDAVPFQGALHG"
     gene            3218..5362
                     /locus_tag="VFF98_12735"
     CDS             3218..5362
                     /locus_tag="VFF98_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64542.1"
                     /translation="MSAHAQTAAPQAPQQLEADIVVTAEKVERRAQDTPITISAVTGE
                     AILREARTKLDDVLANAPGVNVQGVSRGFMVSIRGLGLNLPAQNGAGAVATNYDGVYN
                     SRGEAASTGFYDLERVESLSGPQSTIYGRNAVGGVVNIISHNPTFDRISGYAQGELGN
                     YSNRRIEGALNLPITSNLALRVAAAGVSRDGYISNGHDDNKASSFRAKLLYKPVEPVS
                     ILLGYEQTTLGGKGPGAIPDASYETGSRTTTDPSEGYQHHIARKYWAELKADIGPGQL
                     VVQPSYQTLHGFVQGAFGGVFANNWDPLYTDQLAIEARYSSQKGAKVPWNIGYYHYHN
                     KGTGPTTVSGACYVYSAAGGAGYNTVYIPAAGYSNAAASNGTGCSTTTYQSDYGVDMR
                     TTNVNSAFGQFTVPLIASLRLTLGARESWVQSGGTSDLNSGNTLGATGTALTTSDYVA
                     LAEISKKFFDYRISLEGRIAPRSLVYATVASGHREGGYSFVTGATATTAGVAPKAYDP
                     ESMVDYEIGIKNTLLHGALLLNADAFYYDYKSYQLVYFNPAVGITTPQFLTFPGREYG
                     AEASAAFAITRVDNLTLSIVYLNSKLLGSSAYAGSPFTNAPKWSYKASYGHRFELGDY
                     GTLSARADFRALSSQLVYPTFTADIAGSDTVMGAYATGDFLLSWESANRRYGISAYVK
                     NVNDKLIKSSEFFGYAQVQAPRTYGVTAKVSF"
     gene            5433..6848
                     /locus_tag="VFF98_12740"
     CDS             5433..6848
                     /locus_tag="VFF98_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64543.1"
                     /translation="MIRSRSLSAALLCAGALLAAPLHAQPGAGGPPPGPGGPGGPPPA
                     MPTMADTHYDTTIEVREGKLVKGAASARLGATFSQGAAIASARDGLNGVFISGGHSAF
                     TLARAHIRLAGMGRNDFLGIGAGALVRDNAALVIDHADIDTRGATASAVVAAEGATLR
                     VYDSHLATHGGPLPKGYVRHIGPGMMEPPAPLGLDGDARTVLAMSNSRSFFYRTTIEA
                     DGWGALSTDATGGHLYAEANDCTLIVHQRGYGAYADFGAHVVINRSKVDSGGEVGIIA
                     GAARIDLNRVTGKAGRYGVMIHSVMGDPQEVAELNLADTSLVTTGPAFLVKSANARIT
                     IHGGQIASAAGRLLELRRNEDANATQTHGATVPGVTLTLAAADLAGDVVNSDGDRALR
                     LRLEGARLSGALHHVVLAADAASHWRATAASEVALAPGAALGAIDAAAGITITATTQD
                     PALVAGPRPLPSGGVLLITAG"
     gene            6885..>8307
                     /locus_tag="VFF98_12745"
     CDS             6885..>8307
                     /locus_tag="VFF98_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64544.1"
                     /translation="MHRAKLTSILLGASLLPLAAPALHAETAPASLAASATYRDLTIL
                     PGQIPAAAPGRLLTLTVDGVERPLAPGRYTGAVALSVTDDIPVKYHELPEHHFRAAAY
                     VEDGQLVVAKSVLPALSAHAVIGNGVLSGASIASEGERFNGIIVAGKGAYRIDHPVID
                     LVGNGGNDFAGFGAAITAKGEADLTIDRPIIRTHGAIRTALFVGGHATVHVNDAEIEV
                     INGTLPADYKFTVDIGKMMEVPWMLGMTGNVRATNLVDYGTVYYNRSHIRAQGWGAAS
                     TDDNTRVRMFVNDSLVETIESGYGCYSIGDSVDTFTHSIVRAADVGCIMAANGSVTFS
                     EGTRVQSGRYGVMMHSGAGGGTLLIDKGSELAAASTAIEVKGIGTTIRVDGASLRAGN
                     GILLQSMDNDDPFLVALMSGHLPEGMTAMPPGMGPDGDHADKKGPQPDPDVVASFRNT
                     SLAGDMYNARPKGSAMRLSFEGAA"
BASE COUNT         1342 a         2973 c         2581 g         1411 t
ORIGIN      
        1 catggcgctg atcgacaggg cggggctgct ggcgctgcgc cgcgccgcgc ccttccccgc
       61 gccccctgcc ggagagcgcg cggaggactg gcgcttcgag gtggaaatcc actttggcga
      121 ggcgccctga cgggcccgct caatccagca gcgcgcgcac cttgcccagc aggccggcaa
      181 tgcgctcggc cgcctcgcca aaggtcttca catcctcgcg gttggcattg ccgcgcgctt
      241 ccttggcgga ttccagatag ccttccatat ccgcttccag cgcatcgacg atcatctcgg
      301 cctcgtcctc ggtcagcgtc agtttgattg ccttggtcac gcgattgttc cagtccttgt
      361 cggccagcag gagagcgcca gcgcccttgt tcgagccgcg ctcataggcg cggggggcgc
      421 cgttcgtcca gttgcgcaag gggccttatc aaccgcccgc tggcggtatc tggccgccgc
      481 tctggcgcag caccagctgg ccctccagcc ggcggatgcc atcggccagc ggctgcccct
      541 cgatctgcgc atagagcatc tccagcgttg catcgaccat cgcttccagc ggctgggcat
      601 aggtggccag cgcaaaggcc gggcggcggc ccaccggcac gtcgtcaaag ccgaacaccg
      661 ccacctcgca ccccggctcc agccccatgt cgcgcaccgc ctccagcgcc gacagcgcca
      721 tgaaatcggt gacgcagaac aaagcctcca ccggctggcg cagggagagc agcttgcgcg
      781 ccgcggcata ggcgccctcg gccgaaaact ggccgcaggc ggtgaacagg caggcctcgg
      841 gatgttcggc gatggcgcgg gtgaaggcgg ccgaacgctc cacgctggtc gagctttcct
      901 ccagcccgct gacaaggccg atgcgccgcc gcccgctggc cagcagatgg ccggcgatcg
      961 cccgtgtgcc cgcctcggca tcggtgcaca cgcccggaat gcgcccctcg ggatcgacat
     1021 tgttgagcat caccaccggc acgccgcctt ccaggcattc gcgcgccacc cgcgccgccc
     1081 ggctgctgga cgccaggatc accccatcca gccgataggc cagcaattca tccagcaccg
     1141 gatcaagatc ggcatcgcca tgggtgatga acagcaccag ccggtatccg gccgcagcca
     1201 gcttgtggtg caggcgctcc accatcagcg gatagaacag attgtcgagg ctggtgatcg
     1261 ccaggccaat cagatgcgag cggttggtag tgagcgagcg ggcgctgttg ctgggccgat
     1321 agcgcagcag ccgcgccgcc tccatgatct tgcggcgcgc atcctccgac acgatccccc
     1381 cggtaaacgc ccgggatacc gccgatttcg aaacgcccgc cacccgggcc acatccattc
     1441 cggtaggccg tcccttcgcc acgccgcctt tgcccccttg atccagccgc gcccgagcat
     1501 tgcacaggcg ggccgcaaaa atccatcatc cattttgctg gataggcgcc cattggcgaa
     1561 ttggatttta caatggaagc caagcacgtt cagataagga aaataaattt gggagattct
     1621 cactgatgct gaagaaatct gtcttttgcg tccatcgctg ggatcagttg cagtaatcgt
     1681 cagacagatc gatccgccgc ccttcgtggc cggccgggga tagcctgccg cgccccccgc
     1741 gcctgcccat ccgggacccg acaccctcgc ctgccggctg ctgcgcgcag cctctgcgcg
     1801 tgcccttggc gcgcgccctt cgccaaggaa cccgcatgac caaacaccac catccaggca
     1861 tcatgacagg cgtggccctg ctgctgccga tcaccctttc caccatggcg atcgtgctgc
     1921 tggcgccagt tctgcccaag atccttgaag aattcgcggc cgtgcccggc catgattatt
     1981 gggtgccgat gatcctgacg atcccggcgc tgtgcattgc gctgttctcc acgctggccg
     2041 gcgcgctggg cgatttcttc ggccggcgcc gcctgctgat cgcctctttc tgcgtttatg
     2101 cggtggtcgg cattgcgccg ctgttcctgc atgatctcac cgccatcctc gtcagccggg
     2161 tgggcgtggg gctggccgag gcgctcatca tggtgctctc caccacgatg atcggcgatt
     2221 atttccacgg cgccgcgcgc gacaagtggc tggccgcgca aaccgccttt gcctcgatgt
     2281 cggcgctggt gttcttcaat gtcggcgggc agctgggcgc ctttggctgg cgcacgccgt
     2341 tctgggtcta tctgtcggcg ctggtgatgc tggtgctggt gctggccttt acctgggaac
     2401 cgcagggcga cacccatgac gacgatatca gcgacgaggc cgaggcagcg ccccacaatg
     2461 ccagttgggc cggctttccc tgggcacgca tggccctcat tctggcgatc accatctatg
     2521 gcgcagtatt cttctacacg gtgcagatcc aggcggccgg cggcctggcg ctgctgggcg
     2581 tgaacagccc ggcccaggcc ggcttcctca cctccatcgc cagcatcggc gtgccgctgg
     2641 gcacctttat ctactcacgc gtgggccgcg cgcccgtgca gcgcctgctg atggccgaat
     2701 tcgcgctgct ggcggtgggc ttcacggtga tgggccgcgc cccctcgccg catctgttcg
     2761 tgctgggctg cttcatcaac cagctgggcg ccgggctgct gctgcccacg ctgctggtgt
     2821 gggcgatgag cctgctggcc ttcgagatcc gcggccgcgg cgccggcatg tggcagagcg
     2881 ccttcgcgct gggccagttc ctcagcccgg tggtcgtcac ctcggccgcg ctgcgcaccg
     2941 gcggcctgca gaacgccttc accgtgctgg ccgccggcgc cctgctgggg ctggtggtgg
     3001 tcacgctggc cggccgccgc atccgccgca gcgatgatgc cgtccccttt cagggcgcgc
     3061 tgcatggctg aatgaggcgc tgcacgatct gacatcaagc ccccccggca agttcataaa
     3121 aacgccgggt taatcgagca aaagtttcca agggagaggt attatgaaat atctgtacag
     3181 ggctcttctt ctgtcttcgg cgttgggatc gatcccattg tcagcccacg cccagacagc
     3241 cgccccgcaa gccccccagc agctggaagc tgacatcgtc gttaccgccg aaaaggtgga
     3301 gcgccgcgcg caggatacgc cgatcaccat ctcggccgtt acgggcgagg ccatcctgcg
     3361 cgaggcgcgc accaagctgg atgacgtgct ggccaatgcg ccgggcgtga atgtgcaggg
     3421 tgtctcgcgc gggttcatgg tgtctatccg cgggctgggc ctcaacctgc cggcgcagaa
     3481 cggcgccggc gctgtcgcca ccaactacga tggcgtctat aattcgcgcg gcgaggcggc
     3541 ctccaccggc ttctacgatc tggaacgcgt ggaatcgctg agcggcccgc aaagcaccat
     3601 ctatggccgc aacgcggtgg gcggcgtggt caacatcatc agccacaacc ccactttcga
     3661 tcgcatttcg ggctatgccc agggcgagct gggcaattat tcgaaccgcc gcatcgaagg
     3721 cgcgctcaac ctgccgatca cctccaacct ggcgctgcgc gtggccgcgg ccggcgtatc
     3781 gcgcgatggc tatatctcca acggccacga cgacaacaag gcctcctcct tccgcgccaa
     3841 gctgctctac aagccggtgg agccggtctc gatcctgctg ggctatgaac agaccacgct
     3901 gggcggcaag gggccgggtg ccatccccga tgcctcctac gagaccggct cgcgcacgac
     3961 caccgatccc tcggaaggct atcagcacca catcgcccgc aaatactggg ccgagctgaa
     4021 ggccgacatc ggccccggcc agctggtggt gcagccctcc taccagacgc tgcacggctt
     4081 tgtgcagggc gccttcggcg gcgttttcgc caacaactgg gatccgctct acaccgacca
     4141 gctggcgatc gaggcgcgct acagctcgca gaagggcgcc aaggtgccgt ggaacatcgg
     4201 ctattaccac tatcacaaca agggcaccgg ccccaccacc gtgtcgggtg cgtgctatgt
     4261 ctattcggcc gccggcggag cgggctacaa caccgtctac attcccgccg ccggctacag
     4321 caatgccgcc gcctccaacg gcacgggctg ctccaccacc acctaccagt cggactatgg
     4381 cgtggacatg cgcacgacca atgtgaactc ggccttcggc cagttcaccg tgccgctgat
     4441 cgcctcgctg cgcctcaccc tgggcgcgcg cgaaagctgg gtgcagagcg gcggcacctc
     4501 ggatctcaac agcggcaaca cgctgggcgc caccggcacc gcgctcacca cttccgatta
     4561 tgtggcgctg gccgagatca gcaagaagtt cttcgactat cgcatctcgc ttgaagggcg
     4621 cattgccccg cgctcgctgg tctatgccac ggttgccagc ggccaccgcg agggcggcta
     4681 ttccttcgtc accggcgcca cggccaccac ggcgggcgtc gcgcccaagg cctatgatcc
     4741 ggaaagcatg gtcgattacg aaatcggcat caagaacacg ctgctgcatg gcgcgctgct
     4801 gctcaacgcc gatgccttct attacgacta caagtcgtat cagctggtct atttcaaccc
     4861 ggcggtgggc atcaccacgc cgcagttcct caccttcccg ggccgcgaat atggcgcgga
     4921 ggcctcggcc gcctttgcca tcacccgggt ggacaacctc accttgtcga tcgtctatct
     4981 caactccaag ctgctgggca gcagcgccta tgccggctcg cccttcacca atgcgcccaa
     5041 gtggtcctac aaggccagct atggccaccg cttcgagctg ggcgactatg gcacgctgag
     5101 cgcccgggcc gatttccgcg cgctgtcctc gcagctcgtc taccccacct tcaccgccga
     5161 catcgccggc tccgacacgg tgatgggcgc ctatgccacg ggcgatttcc tgctctcgtg
     5221 ggaaagcgcg aaccggcgct atggcatctc ggcctatgtc aagaacgtga acgacaagct
     5281 gatcaagtcg tctgaattct ttggctacgc gcaggtgcag gcgccgcgca cctatggcgt
     5341 cactgccaag gtcagcttct gatcggccgg ggcgcgcaag gccctgcggc catgcgcgcc
     5401 cccttctccc ccccgagaac aaggatatac cgatgatccg ctcgcgatcc ctttctgccg
     5461 cgctgctgtg cgctggcgcg ctgctggctg cgccccttca tgcccagccc ggcgctggcg
     5521 gcccgccgcc tggccccggt ggccccggcg gcccgccgcc cgccatgccc accatggccg
     5581 acacccatta cgacaccacc atcgaagtgc gcgaaggcaa gctggtgaag ggcgccgcca
     5641 gcgcccggct gggcgccacc ttcagccagg gcgcggccat tgcttccgcg cgtgacgggc
     5701 tgaacggggt cttcatcagc ggcgggcaca gcgccttcac gctggcccgc gcccatatcc
     5761 gccttgccgg catgggccgg aacgatttcc tcggcattgg cgccggcgcg ctggtgcgcg
     5821 acaatgccgc gctggtgatc gaccatgccg atatcgacac ccgcggtgcc accgcttctg
     5881 ccgtggtggc ggccgaaggg gccaccttgc gggtgtatga ttcgcatctg gccacccatg
     5941 gcgggccgct gcccaagggc tatgtgcgcc acatcggccc ggggatgatg gagccgcccg
     6001 ccccgctggg cctggatggc gatgcccgca ccgtgctggc gatgagcaat tcgcgcagct
     6061 tcttctatcg caccaccatc gaggccgatg gctggggcgc cctttccacc gatgccaccg
     6121 gcggccacct ctatgccgag gcgaatgact gcacccttat cgtgcaccag cgcggctatg
     6181 gcgcctatgc cgatttcggc gcccatgtgg tgatcaatcg cagcaaggtg gatagcggcg
     6241 gcgaggtcgg catcatcgcc ggcgcggccc gcatcgacct caatcgggtg accggcaagg
     6301 ccggccgcta tggcgtgatg atccattcgg tgatgggcga cccgcaggaa gtggccgagc
     6361 tgaaccttgc cgacaccagc cttgtcacca ccggccccgc cttcctcgtc aaaagcgcca
     6421 atgcgcgcat caccatccat ggcggccaga tcgccagcgc cgccggccgc ctgctggagc
     6481 tgcgccgcaa cgaggatgcc aatgccacgc agacccacgg cgccacggtg cccggcgtca
     6541 ccctcaccct ggcagcggcg gatctggccg gcgatgtggt gaacagcgat ggcgatcggg
     6601 cgctgcggct gcggctggaa ggcgcgcggc tgagcggcgc gctccatcac gtggtgctgg
     6661 ccgcagatgc cgccagccac tggcgcgcca ctgccgcctc cgaagtggcg ctagcccccg
     6721 gcgctgccct tggcgcgatc gatgcggccg ccggcatcac catcaccgcc accacgcagg
     6781 atccggccct tgtcgccggg ccccggccgc tgccttccgg cggcgtgctg ctgatcaccg
     6841 ccggctgacc ggccccgctt tccccccacg aagagaccag acccatgcac agagccaagc
     6901 tgacttccat ccttctgggt gcctcgctgc tgccgctggc agcccctgcg ctgcatgccg
     6961 aaaccgcgcc ggccagcctt gccgccagcg ccacctaccg cgacctcacc atcctgcccg
     7021 gccagatccc cgccgccgcc cccggccgcc tgctgacact gacggtggat ggcgtggagc
     7081 gcccgctggc acccggccgc tacaccggcg ccgtggcact gagtgtgacc gacgacattc
     7141 ccgtgaaata ccacgagctg cccgaacacc atttccgcgc cgccgcctat gtggaggatg
     7201 gccagctggt ggtggccaaa tcggtgctgc cggcgctgtc ggcccatgcg gtgatcggca
     7261 atggcgtgct gtcgggcgcc agcatcgcct ccgagggcga gcgtttcaac ggcatcatcg
     7321 tggccggcaa gggcgcctat cgcatcgatc atccggtgat cgatctggtg ggcaatggcg
     7381 gcaatgattt tgccggcttt ggcgcggcga tcaccgccaa gggcgaggcc gacctcacta
     7441 tcgaccggcc gatcatccgc acccatggcg ccatccgcac ggcgctgttc gtgggcggcc
     7501 atgccaccgt ccatgtcaac gatgccgaga tcgaggtgat caacggcacg ctgccggccg
     7561 attacaagtt caccgtcgat atcggcaaga tgatggaagt gccctggatg ctgggcatga
     7621 ccggcaatgt gcgcgccacc aatctggtgg attatggcac cgtctactac aaccgctcgc
     7681 atatccgcgc gcagggctgg ggcgctgcct ccaccgacga caacacccgc gtgcgcatgt
     7741 tcgtcaacga cagcctcgtc gagacgatcg agagcggcta tggctgctat tccatcggcg
     7801 acagcgtgga taccttcacc cattcgatcg tgcgcgcggc cgatgtcggc tgcatcatgg
     7861 cggccaatgg cagcgtcacc ttcagcgagg gcacccgcgt gcaatcgggc cgctatggcg
     7921 tgatgatgca ttcgggcgcc ggcggcggca cgctgctgat cgacaagggc agcgaactgg
     7981 ccgcggccag caccgcgatc gaggtgaagg gcattggcac caccatccgc gtcgatggcg
     8041 ccagcctgcg cgcgggcaat ggcatcctcc tccagtcgat ggacaatgac gatcccttcc
     8101 tggtggcgct gatgagcggc cacctgcccg aaggcatgac cgccatgccc cccggcatgg
     8161 gcccggatgg cgatcatgcc gacaagaagg gcccgcagcc cgatcccgat gtggtggcca
     8221 gcttccgcaa caccagcctt gccggcgata tgtacaacgc tcgccccaag ggcagcgcca
     8281 tgcgcctctc cttcgagggc gcagcga
//
LOCUS       DASRYE010000252        19125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_29964,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000252 DASRYE010000000
VERSION     DASRYE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29964"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /gene="bamA"
                     /locus_tag="VFF98_12750"
     CDS             complement(<1..1940)
                     /gene="bamA"
                     /locus_tag="VFF98_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZU64545.1"
                     /translation="MGHNAGIYRISHLGVALLGSSILAGVPQMAQARAPRAEAIDPAP
                     APAPAKPAAVAVPAAASTAPAVIIRSISVEGAQRLEGDTVLSYIHLRVGDAYTEVVAD
                     QALKDLYATELFADAQVRNDNGHVVITVKENPVINRIILEGNKHLKEDKINPEIKVAP
                     RQIFTRSKVRADVARIIELYKRQGRFGASVEPKMVKLDQNRVDIVFEINEGPKSKVRQ
                     INVIGNEAFNDTQLRDVMMTKQARPLHVFRGGTGYDPDKLAYDQQKLRQFYLTQGYAD
                     FRVVSAVAELTPDKRDFIITYVIEEGKRYKFGDVKVESQLRDFDSSALTKALPMHKGD
                     WYNAKEVEDTIDQMNTAAGGFGYAFADARPSYQRDAKTLTMGVTFNIGEAPRVYVERI
                     DVNGNTLTQDKVIRREFRLAEGDAFNASQVKRSTNRIKSLGYFQEKFEVKQVRVSDDR
                     INLEANLEEKPTGELQLSAGYSSLESLIFQASVQQHNFRGRGQTVGLSGSISRYSKSV
                     TLSFTEPYLFDKNVSLGVDIYRRDYNNYNYYSSNSNTLYSNSTTGFQVRLGMPVTEYV
                     SLIGRYTLNYDNVSLDKSTYYANDPVTKSYGCSALLAGRYLCEAVGKRLSSIVGSSLV
                     FENVDNRAHPTKGRIASVGFDLA"
     gene            complement(2153..3289)
                     /gene="rseP"
                     /locus_tag="VFF98_12755"
     CDS             complement(2153..3289)
                     /gene="rseP"
                     /locus_tag="VFF98_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZU64546.1"
                     /translation="MGAGALAHLAQPSLLLILVAFALLLGPLVMLHELGHYAMGRLFG
                     VKADAFSIGFGRELIGWTDRRGTRWKVSMLPLGGYVQFAGDANAASLPTLPPADRAAA
                     AAAGFFHAKPLWQRALVVLAGPLANLLIAVTIFATFNLVYGRIEALPVVAGLAPGSAA
                     EVAGLKLDDRIVAIDEAPITSFEDIRAHVMPYPGAHLRLTVARGGQQLVLPVTVGTHV
                     ETDAYGNEARIGMLGVASGRISRVAVGPVGAVGLAFRQCADVMGMMTTGIGQIIGGQR
                     SVKELGGPVKIAQYSGQQLAQGWLNFVDFAALISINLAFINLLPIPMLDGGHLAFYLA
                     EAVRRRPVDLRMQEWAYRMGLALVLGLMLFVTLNDLASLHLFGR"
     gene            complement(3293..4465)
                     /locus_tag="VFF98_12760"
     CDS             complement(3293..4465)
                     /locus_tag="VFF98_12760"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZU64547.1"
                     /translation="MTHPHTPRSISVLGATGSIGASTLDLIRRNRADWRVVALTANGN
                     AAALAELAREFAAEVAVVADEAALPALREALAGTGIAAAAGTAALVEAAARPADLTMA
                     AIVGCAGLAPTMAAIEQGRTVALANKEALVSAGEVMLAAVRAHGTTLLPVDSEHNAIF
                     QCLSGNKTDHVRWITLTASGGPFRDWSLEQLHTATPAQAIKHPNWSMGAKISVDSATM
                     MNKGLEFIEAYHLFPVGLDRLRIIVHPQSIIHSMVEYVDGSTLAQLGPSDMRVPIASC
                     LAWPERMATPLSSQLDLAAIGELTFRAPDETRFPATRLARQAAHAGGGIPAVLNAANE
                     VAVDAFLKGRIGFTRIAAMVEDVLGRYAPPPPVALEDVHAVDHEARLRAGALLELA"
     gene            complement(4466..5221)
                     /locus_tag="VFF98_12765"
     CDS             complement(4466..5221)
                     /locus_tag="VFF98_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZU64548.1"
                     /translation="MADGEPRAGRFAADLKQRTLSAVVMVALAGGALWLGGPVWQSFV
                     LLVGAGVCWEWSRLAARISRNAFARLAWLAAAVVYVGMGVEMLLRLRLDGPLPVLVIV
                     LAVMATDIGAYFAGRTFGGPKVAPKISPSKTWSGLGGAMLAAGLVLALLIAWLCPAQS
                     QPGAWLQPLPVRWTGPFWALEARCMGAGAVIALIAQTGDFFESWMKRRAGVKDSSNLI
                     PGHGGLFDRLDGLLAVLFVAGGLALASALAGRF"
     gene            complement(5208..5852)
                     /gene="uppS"
                     /locus_tag="VFF98_12770"
     CDS             complement(5208..5852)
                     /gene="uppS"
                     /locus_tag="VFF98_12770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZU64549.1"
                     /translation="MDGNGRWAKKRMLPRAVGHSRGVETVRRLVRAAPGLGIDVLTLY
                     AFSCENWRRPADEVSDLMGLLKRFILSDLDEIHANGVRLRILGDYKAMATDIVTLLDN
                     AIARTAGNTRMTLAIALNYGSQQEIARAAARAAEKGPITPEAIEAELDTADLPPLDLM
                     VRTSGEVRLSNFLLWQAAYAEMWFTPVLWPDFTPEHLAEAMSAFGQRERRFGGR"
     gene            complement(5915..6478)
                     /gene="frr"
                     /locus_tag="VFF98_12775"
     CDS             complement(5915..6478)
                     /gene="frr"
                     /locus_tag="VFF98_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZU64550.1"
                     /translation="MAAPKYDKADLERRMKGAVESLKGDLSGLRTGRANIALLDPITV
                     EIYGSHMPLNQVATVATPEPRMLTVTVWDKSSVGPVEKAIRSAGLGLNPINDGNTLRL
                     PIPDLTEERRKELAKLASKYAEAARIAIRNVRRDGMEALKADENKKLISEDERKRAET
                     EVQKLTDEIVKAVDEVVSAKEKEILGK"
     gene            complement(6491..7210)
                     /gene="pyrH"
                     /locus_tag="VFF98_12780"
     CDS             complement(6491..7210)
                     /gene="pyrH"
                     /locus_tag="VFF98_12780"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZU64551.1"
                     /translation="MQLPTYKRILLKLSGEVLMGDQQFGIDPAFVAELAKEVKAARDT
                     GLEICLVIGGGNIFRGMAGAARGMDRAQADYMGMLATIMNALAMQNALEQLGVPTRVQ
                     SAIQMDEVCEPVIRRRAERHLEKGRIVIFAAGVGSPYFTTDSGAALRAAELNCDALLK
                     GTSVDGVYNADPKKDPEARRYDTVDYDTVLADNLKVMDASAVALCRDNKIPIVVFSIR
                     ERGNLARVLAGEGTQTVVQQA"
     gene            complement(7482..8408)
                     /gene="tsf"
                     /locus_tag="VFF98_12785"
     CDS             complement(7482..8408)
                     /gene="tsf"
                     /locus_tag="VFF98_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZU64552.1"
                     /translation="MAYTAADVKNLRERTGAGMMDCKKALDETGGDFEAAVDALRAKG
                     LAAAAKKSSRTAAEGLVGVAVAGTAGIAVEVNSETDFVAKNDQFQDFVRKATEVALGG
                     PISDVEALKAAAYPDGGTVGEKLTSNVATIGENQQIRRIKAVQVKAGVVVPYMHNAAA
                     PNLGKIGVLVALESEAGADVLEPLGKQIAMHIAAAFPLALNADDLDAELIERERKIAA
                     EKAAESGKPAEVQAKMVDGAVAKYAKENALLSQLFVMDNKNTVQQVVDAAGKAAGAKI
                     ALVDYVRFQLGEGIEKEASDFAAEVAAAAGLA"
     gene            complement(8544..9299)
                     /gene="rpsB"
                     /locus_tag="VFF98_12790"
     CDS             complement(8544..9299)
                     /gene="rpsB"
                     /locus_tag="VFF98_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZU64553.1"
                     /translation="MAAPVVSMQQLIEAGAHFGHQTHRWNPRMKPYIFGARNGVHILD
                     LSQTVPLFARALDFVSATVQAGGKVLFVGTKRQAQEPIAQAARACGQHFVNHRWLGGM
                     LTNWKTISQSIKRFKALEEKLSGDTAGLTKKEVLQMTRERDKLELSLGGIRDMGGIPD
                     VMFVIDANKEELAIKEANVLGIPVVAILDSNVSPEGIAFPIPANDDASRAIRLYCEAV
                     ASAATKGGRDAVVDSGADIGAFENPLAEEAIEG"
     gene            9687..10091
                     /locus_tag="VFF98_12795"
     CDS             9687..10091
                     /locus_tag="VFF98_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64554.1"
                     /translation="MPISPAAAMHSLSVPGLAADDLRTWVAPMLAMVALLAISVLGAS
                     LMKAARLFAVLREEALAAGAERPIALALRPAAMPAAWQDSEMPAVWQASQMAAAWQAG
                     PVAACREEWVNRPMRQALRPAPQQTGAMPAAA"
     gene            complement(10021..10857)
                     /locus_tag="VFF98_12800"
     CDS             complement(10021..10857)
                     /locus_tag="VFF98_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HZU64555.1"
                     /translation="MSRPSRAGFSREGAAGRFPGGLTLRALIPNAITSSALCCGLTAI
                     RFAIQGDWEKSVLAVILAGVLDGIDGRIARLLKAQSRFGAEMDSLSDAISFGVAPALI
                     LFLWSLQQAPRLGWFAALAFALCCVLRLARFNARIDMPEQPHKSAGFLTGVPAPMGAA
                     LGMLPLYLWISSDNPLFRQPQVVGIWLVVIAFLMISSLATFGWGALRPRRAVRLELIA
                     LFGLVFAALFSEPWLTLAGLCIAYIALLPIGLVVYGRVKRQRASRQSAAVQGAAPGAS
                     AD"
     gene            complement(10854..11603)
                     /locus_tag="VFF98_12805"
     CDS             complement(10854..11603)
                     /locus_tag="VFF98_12805"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZU64556.1"
                     /translation="MPGEILDNRGHGAASWHWPSVHPEARKFAAVAAIGCLLAAIFGL
                     SLLAWPLGILTYCICAFFRDPQRVTPRGEGFIVAPADGLVTLIQKVPPPRELSTDDGS
                     GLPCLGTQPVTRISIFMSVFDVHINRAPIGGTVRRVVYIPGRFLNADLDKASEENERQ
                     HILIDRGQGVLIGFTQIAGLVARRIVPFVKPGDIIASGQRVGLIRFGSRVDVYLPEGT
                     EPAIILGQRIVAGETILATMGSQPLIEGISQ"
     gene            complement(11717..11802)
                     /locus_tag="VFF98_12810"
     tRNA            complement(11717..11802)
                     /locus_tag="VFF98_12810"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11766..11768),aa:Tyr,seq:gta)
     gene            11965..12795
                     /gene="rlmB"
                     /locus_tag="VFF98_12815"
     CDS             11965..12795
                     /gene="rlmB"
                     /locus_tag="VFF98_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZU64557.1"
                     /translation="MGKNGEEGGGRREGRLLRGRAGRMQGGRGSGRGSKGAVRLWGRH
                     AVEAALKNPERRHRKLWATREGIASLDGELPADFPLEYAQAADLGRLVAKDAPHQGLV
                     LDCEPLEDIYLDDVLSGDPARPLVVLDQVTDPHNVGAILRSAAAFDACAIVTQDRHSP
                     PESGTVAKSASGALEIVPWVRVVNLARALEEIAEAGYWRIGLAGEARSTLAAALPAGP
                     VALVLGAEGEGMRHNITQHCDALARLPISSAMESLNVSNAAAIALYAAASRADIAEED
                     "
     gene            complement(12949..13221)
                     /locus_tag="VFF98_12820"
     CDS             complement(12949..13221)
                     /locus_tag="VFF98_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZU64558.1"
                     /translation="MNKNDLIGAVAEASGLTRNDATKAVEGVFDAITSALKNGDEVRL
                     VGFGTFSVAKRKASTGRNPRTGEPMPIAASAQPKFKAGKGLKDAVN"
     gene            complement(13464..15863)
                     /gene="lon"
                     /locus_tag="VFF98_12825"
     CDS             complement(13464..15863)
                     /gene="lon"
                     /locus_tag="VFF98_12825"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZU64559.1"
                     /translation="MDVTSLPLLPLRDIVVFPGMVVPLFVGREKSVAALEAAMAGNKT
                     IFLLAQLDPGCDDPQADDLYDVGVVASVLQLLKLPDGTVRVLVEGQSRAKLAGLKEEP
                     TLLMADVADIEPETAEGPEVAAMMRSVVDQFHEYAKLNKKLSQEAGDQLGDIEEASKL
                     ADAVAAHISAKVSDKQALLSEIDPLKRLEMVYGLMEGELGVLQVERRIRGRVKRQMEK
                     TQREYYLNEQLKAIQSELGGGDDGEANEIAELTEKIAKLKLSKEARAKAEAEVKKLKT
                     MQPMSAEATVVRNYLDVLLGLPWGKKSKLKKDIGAAQAILDADHYALDKVKDRIVEYL
                     AVQARTNKLKGPILCLVGPPGVGKTSLGKSIARATGREFIRQSLGGVRDEAEIRGHRR
                     TYIGSMPGKIVSNLKKAGTSNPLFLLDEIDKLGQDFRGDPASALLEVLDPEQNAKFQD
                     HYLELDIDLSDVMFVCTANSLNLPQPLLDRMEIIRLEGYTEDEKLEIAERHLIPRQVQ
                     QHGLKKGEFELTSAALRDLIRYYTREAGVRTLDREIARLARKSLRRILEGKETGVVIT
                     PENLADFAGVRKFRHGMSDDENQIGAVTGLAWTEVGGELLTIESVTVPGKGAIKTTGK
                     LGEVMTESVQAAFSFVKARAPAYGIKPSIFNRKDIHIHLPEGAVPKDGPSAGIGMVTA
                     MVSTLSGIPVRADVAMTGEVTLRGRVLAIGGLKEKLLAALRGGIKTVLIPEENQKDLA
                     EIPANIKAGLEIIAVAHVDEVLARALAQPVAPIEWTEADDLASQPGALPAPGSPAATA
                     H"
     gene            complement(16056..16391)
                     /locus_tag="VFF98_12830"
     CDS             complement(16056..16391)
                     /locus_tag="VFF98_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZU64560.1"
                     /translation="MKSMEEMIAAAQQAAETISKQMNEAQGRLDSLEVEGVAGGGLVR
                     IRASAKGRVIGVAIDDSLLAPSEKGILEDLVAAAFNDARAKADEVAGAELAKAQAGMG
                     LPPGFKLPF"
     gene            complement(16392..18200)
                     /locus_tag="VFF98_12835"
     CDS             complement(16392..18200)
                     /locus_tag="VFF98_12835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZU64561.1"
                     /translation="MGDSPQHTASTEAGQAQEALPTAAELEAAGQSALFGGPAPAAQA
                     APPVVAKPAASQPYRVLARKYRPQTFSALIGQDAMVQTLANAIKRDRLAHAFLMTGVR
                     GVGKTSTARLIAKALNCVGADGQGGPTIDPCGQCEPCRAIAEGRHIDVIEMDAASHTG
                     VDDVREIIEAVRYAAVSARYKIYIIDEVHMLSRNAFNALLKTLEEPPAHVKFLFATTE
                     VDKLPITVLSRCQRFDLKRIPAPLLATHFAGICAAEGVEAEAPALAMVAQAAEGSVRD
                     GLSILDQAIAHADMAAAGAATLVTAEQVRDMLGLADKGAQRRLFAALLAGEGADLLDL
                     VANQYALGVEPLALMRGAMELAHRVAVAQLGRGDVRAVSDEERAAIEDWGKRLSAGQV
                     HRLWQLLLKGHEEVKGAPDPLVAAEMALLRCLHAADLPDPGQLARRIEQMAASGAVLA
                     AAGGPGPDALPGGGAEALAPALSWDALVEQVEESGLPGGMTMASMMRVQVRVIELAQG
                     RLVFAQPPEFRDDLGPEMRAMLQKVSGQLWHVERQAEGGAPTLDEAHRADVRARGEAE
                     RAAPLVAAALAAFPAAEIIRDEPGAPQRPRQANWRN"
     gene            complement(18289..18383)
                     /gene="ffs"
                     /locus_tag="VFF98_12840"
     ncRNA           complement(18289..18383)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFF98_12840"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(18437..18715)
                     /locus_tag="VFF98_12845"
     CDS             complement(18437..18715)
                     /locus_tag="VFF98_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HZU64562.1"
                     /translation="MTARIYQRPKNAMQSGKALLGQWILEFAPAEARHADPLMGWAGS
                     GDTQSQVQLKFHTQGEAQAYADKYGIAAAVHATPPRRLKLQAYADNFR"
     gene            complement(18749..>19125)
                     /locus_tag="VFF98_12850"
     CDS             complement(18749..>19125)
                     /locus_tag="VFF98_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HZU64563.1"
                     /translation="GWEGKQALERLAPAEFRSPAGTSHSIDYDDPGGPSVEVRVQALF
                     GLERHPGFGQPAQPLLLKLTSPGGKPIQTTRDLPGFWRGSWRDVVKEMKGRYPRHRWP
                     DEPWAEDPSLKTKNAFSAQRRT"
BASE COUNT         3170 a         6550 c         6173 g         3232 t
ORIGIN      
        1 gcgaggtcga aacccacaga ggcgatccgc cccttggtgg ggtgcgcgcg gttgtcgaca
       61 ttctcgaaca ccagcgagga gccgacgatc gagctcaacc gcttgcccac cgcctcgcac
      121 agatagcggc cggccagcag cgcgctacag ccataggact tggtgaccgg atcgttggcg
      181 taataggtgg acttgtcgag cgagacgttg tcgtagttca gcgtgtagcg gccgatcagc
      241 gagacatatt ccgtgaccgg catgcccagg cgcacctgga aaccggtggt ggagttgctg
      301 tagagcgtgt tgctgttcga gctgtagtaa ttgtagttgt tatagtcgcg ccgatagata
      361 tccacaccca gcgagacgtt cttgtcgaac agatagggct cggtgaagga cagcgtcacg
      421 ctcttcgaat agcgcgagat gctgcccgag aggcccactg tctgcccgcg gccgcggaag
      481 ttgtgctgct gcacgctggc ctggaagatc aggctttcca ggctggaata gccggccgag
      541 agctgcagct cgccggtggg cttttcttcc agattggctt ccaggttgat gcggtcgtcc
      601 gacacgcgca cctgcttcac ctcgaacttc tcctggaagt agccgaggct cttgatgcgg
      661 ttggtggagc gcttgacctg cgaggcgttg aaggcatcac cctcggccag gcggaattcg
      721 cggcggatca ccttgtcctg cgtcagcgtg ttgccgttga catcgatccg ctcgacatag
      781 acgcgcggcg cctcgccgat gttgaaggtc acgcccatgg tcagcgtctt ggcgtcgcgc
      841 tgatagctgg ggcgggcatc cgcgaaggca tagccgaacc cgccggcggc ggtgttcatc
      901 tggtcgatcg tgtcctcgac ctccttggcg ttataccagt cgcccttgtg catcggcagg
      961 gccttggtca gcgcgctgct gtcgaagtcg cgcagctggc tttccacctt cacatcgccg
     1021 aacttgtagc gcttcccttc ctcgatcaca taggtgatga tgaaatcgcg cttgtcgggc
     1081 gtcagctcgg ccacggccga gaccacgcgg aaatcggcat agccctgggt caggtagaac
     1141 tggcgcagct tctgctggtc gtaggccagc ttgtccggat catagccggt gccgccacgg
     1201 aagacatgca ggggccgggc ctgcttggtc atcatcacgt cgcgcagctg ggtgtcgttg
     1261 aaggcctcgt tgccgatcac gttgatctgg cgcaccttcg acttgggccc ctcgttgatc
     1321 tcgaagacga tatcgacgcg gttctggtcg agcttgacca tcttgggctc gacgctggcg
     1381 ccaaagcggc cctggcgctt gtagagctcg atgatgcggg ccacgtcggc gcgcaccttg
     1441 ctgcgggtga agatctggcg cggggcgacc ttgatctcgg ggttgatctt gtcttccttg
     1501 aggtgcttgt tgccctccag gatgatacgg ttgatcacgg ggttttcctt caccgtgatc
     1561 accacatggc cgttgtcgtt gcgcacctgc gcatcggcga aaagctcggt ggcgtagaga
     1621 tccttcagcg cctggtcggc caccacctcg gtataggcat cgcccacgcg caggtggata
     1681 taggagagca ccgtgtcgcc ctcgaggcgc tgggcccctt ccacgctgat cgagcggatg
     1741 atgaccgcag gcgccgtgct ggccgccgca ggcacggcca cggccgccgg cttggcaggc
     1801 gcgggcgcag gggccggatc gatcgcttcg gcgcgcggcg cccttgcctg cgccatctgc
     1861 ggcacgccgg ccagaatcga actgcccagc agggccacac ccagatgcga gatccggtaa
     1921 attccagcgt tgtgacccat attccgtcca atcatcgaca cggtcgttcc tatcatgtgc
     1981 ctgtcggggt tgcccccggc gcgcgcaccc ccgtctggct atgccatggc gcacccaccc
     2041 gcgcaccctg gcaaacctcg taacggccga acggccctgt gcccgatgcc gcaacggcaa
     2101 tcaaggcatc ttgccggctc gcccacaaac cttttgtcgc aaatccctct gcctagcgcc
     2161 cgaacaggtg cagggaggcc agatcgttga gggtgacaaa cagcatcagc cccagcacca
     2221 aagccagccc catgcgatag gcccattcct gcatgcgcag atccaccggc cggcggcgca
     2281 ccgcctcggc caggtaaaac gccagatgcc cgccatccag catggggatt ggcagcaggt
     2341 tgatgaatgc caagttaatg gaaataagcg ccgcgaaatc gacgaaattg agccagccct
     2401 gcgccagttg ctggccagaa tactgggcga tcttcacagg gccgcccaat tccttcacgc
     2461 tgcgctgacc gccgatgatc tggccgatgc cggttgtcat catgcccatt acatcggcgc
     2521 actggcgaaa ggccaggccc accgcgccca ccggccccac cgccacccgg ctgatccgcc
     2581 cggacgccac gcccagcatg ccgatccgcg cctcattgcc ataggcatcg gtttccacat
     2641 gggtgcccac cgtgaccggc agcaccagct gctggccgcc acgcgccacc gtgaggcgca
     2701 gatgcgcgcc cggatagggc atgacatgcg cgcgaatatc ctcgaagctg gtgatcggcg
     2761 cctcgtcgat cgccacgatc cggtcgtcga gcttcaggcc cgccacttcg gccgccgatc
     2821 ccggcgccag cccggccact accggcagcg cctcgatgcg gccatagacg aggttgaagg
     2881 tggcaaagat cgtcaccgcg atcagcaggt tggccaaggg gccggccagc accaccagcg
     2941 cccgctgcca caggggcttg gcatggaaga agccggcagc ggctgccgcg gcccggtctg
     3001 ccggcggcag ggtgggcagg ctggcggcat tggcatcgcc ggcaaactgc acatagccgc
     3061 ccagcggcag catcgagacc ttccagcgcg tgccgcgccg gtccgtccag ccgatcagct
     3121 cgcggccaaa gccgatggag aaggcatctg ccttcacgcc gaacagccgg cccatggcat
     3181 aatggcccag ctcgtgcagc atcaccagcg gccccagcag cagcgcaaag gccaccagga
     3241 tcagcagcag cgagggctgc gccagatggg ccagagcgcc cgctcccatg gctcaggcca
     3301 gttccagcag cgcgccggcg cgcaggcggg cctcatggtc caccgcatgc acatcctcca
     3361 gcgccaccgg cggcggcggg gcatagcggc ccagcacatc ctccaccatg gcggcgatgc
     3421 gggtgaagcc gatgcggccc ttgaggaagg catccaccgc cacttcattg gcggcattga
     3481 gcacggcggg aatgccgccg ccggcatggg ccgcctggcg cgccaggcgc gtggcgggaa
     3541 agcgggtttc atccggcgcg cggaaggtca gctcgccaat cgcggcaagg tccagctggc
     3601 tggacagcgg ggttgccatg cgttccggcc aggccaggca gctggcgatc ggcacgcgca
     3661 tgtcgctggg gcccagctgg gccagcgtgc tgccatcgac atattccacc atggaatgga
     3721 tgatcgattg cggatggacg atgatgcgca gccgatcgag ccccaccggg aacaggtgat
     3781 aggcctcgat gaattcgagc cccttgttca tcatggtggc cgaatcgacg ctgatcttgg
     3841 cgcccatgct ccagttgggg tgcttgatcg cctgggcggg cgtggccgtg tgcagctgct
     3901 ccaggctcca gtcgcggaac gggccgccgc tggcggtcag cgtgatccag cggacatgat
     3961 cggtcttgtt gccggaaagg cactggaaga tggcgttatg ctcgctgtcc accggcagca
     4021 gggtggtgcc atgggcacgc acggcggcca gcatcacctc gccggcggaa accagcgctt
     4081 ccttgttggc cagcgccacg gtgcggccct gctcgatcgc ggccatggtg ggggcaagcc
     4141 ccgcgcagcc gacgatcgcg gccatggtaa gatcggccgg gcgggcggcg gcttccacca
     4201 gcgccgcggt gccggcggca gcggcaatgc cggtgccggc cagcgcctca cgcagggccg
     4261 gcagcgcggc ttcatcggcc accacggcca cttcggcggc aaattcgcgg gccagttcgg
     4321 ccagggcggc ggcattgcca ttggccgtga gcgccaccac gcgccaatcg gcccggttgc
     4381 ggcggatgag atccagcgtg gaggcgccga tcgagccggt agcccccaga accgagatgc
     4441 tgcgcggagt gtgtgggtgt gtcacttaaa atcttcccgc caatgcgctc gccaatgcca
     4501 acccgcccgc tacgaacagc acggcgagca agccgtcaag cctgtcgaac aggccgccat
     4561 ggcccggaat gaggttggag ctgtctttca cgccggcgcg gcgcttcatc cagctttcga
     4621 agaaatcgcc ggtctgggcg atcagggcga taacggcacc cgcccccatg cagcgcgcct
     4681 ccagcgccca gaagggcccc gtccagcgca ccggcagcgg ctgcagccag gcgccgggct
     4741 ggctttgcgc cgggcacagc caggcgatca acagcgccag caccagccct gcggccagca
     4801 tcgcaccgcc aagcccgctc caggttttgc tggggctgat tttgggagca accttggggc
     4861 cgccaaaggt gcggccggcg aaataggcgc caatatcggt ggccatcacc gccagcacga
     4921 tcaccagcac cggcaacggc ccatcaaggc gcagacgcag cagcatctcc acccccatgc
     4981 cgacatagac caccgccgcg gccagccacg ccaggcgcgc aaaggcgttc cggctgatgc
     5041 gggcggccag ccggctccat tcccagcaca cgcccgcgcc caccagcagc acgaagcttt
     5101 gccacaccgg gccgcccagc cacagcgcgc cgcccgccag cgccaccatc accacggccg
     5161 atagcgtgcg ctgcttcaga tccgccgcaa accggcccgc gcgcggctca ccgtccgcca
     5221 aagcggcgct cccgctggcc aaaggcgctc atcgcctcgg ccagatgttc gggcgtgaaa
     5281 tcgggccaca gcaccggcgt gaaccacatt tccgcatagg ccgcctgcca cagcaggaag
     5341 ttcgacaggc gcacctcgcc gctggtgcgc accatcagat caagcggcgg cagatcggcg
     5401 gtgtcgagtt cggcctcgat cgcctccggg gtgatcggcc ctttctcggc ggcgcgggcg
     5461 gccgcgcgcg cgatttcctg ctggctgcca tagttcagcg cgatcgccag cgtcatgcgc
     5521 gtgttgccgg cggtgcgggc gatggcatta tccagcagtg tcacgatatc cgtggccatc
     5581 gccttgtaat cgcccaggat cctcaggcgc acgccattgg cgtggatctc gtcgagatcc
     5641 gacaggatga agcgcttcag cagccccatc aggtcgctca cctcatcggc cgggcggcgc
     5701 cagttctcgc aggaaaaggc atagagcgtg agcacatcga tgcccaggcc cggcgctgcc
     5761 cgcaccagcc ggcgcaccgt ttccacgccg cggctgtggc ccacggcgcg tggcagcatg
     5821 cgctttttcg cccagcgccc gttgccatcc atgatgatcg cgatatggcg cgggcccccg
     5881 gccctgccgc cacccgccgg agcggccaga gctgtcactt gcccaggatt tccttttcct
     5941 tggccgacac cacctcgtcg accgccttga cgatctcgtc ggtcagcttc tgcacttcgg
     6001 tctcggcgcg cttgcgctcg tcctcgctga tcagcttctt gttctcgtcg gccttcagcg
     6061 cttccatgcc atcgcggcgc acgttgcgga tggcgatgcg ggccgcttct gcatatttgc
     6121 tggccagctt ggccagttcc ttgcggcgtt cctcggtcag atcggggatc ggcaggcgca
     6181 gggtgttgcc atcgttgatg gggttgaggc ccagccctgc cgaacggatc gccttttcca
     6241 ccggccccac gctcgacttg tcccacacgg tcacggtcag catgcgcggc tcgggcgtgg
     6301 ccacggtggc cacctggttg agcggcatgt ggctgccata gatttccacc gtgatcggat
     6361 cgagcagcgc gatattcgcg cggcccgtgc gcaggcccga cagatcgccc ttcagcgatt
     6421 ccaccgcgcc cttcatgcgg cgttcgagat cggccttgtc atatttcggc gctgccatgt
     6481 tcctaccctt tcaagcctgc tgaacgaccg tttgcgtgcc ctcgcccgcc agcacgcggg
     6541 ccagattgcc ccgctcgcgg atggagaaca caacgatcgg gatcttgttg tcgcggcaca
     6601 gggccacggc gctggcgtcc atcactttca gattgtcggc cagcacggta tcgtaatcca
     6661 ccgtatcata gcggcgcgct tcgggatcct tcttgggatc ggcattatac acgccatcaa
     6721 cgctggtgcc cttcagcagg gcatcgcagt tcagctctgc cgcgcgcagg gccgcgccgg
     6781 aatcggtggt gaaatagggg ctgccaacgc cggccgcaaa gatcacgatg cggccctttt
     6841 ccagatggcg ctcggcgcgg cggcgaatca ccggctcgca cacctcgtcc atctggatgg
     6901 ccgattgcac gcgggtgggc acgcccagct gttccagcgc gttctgcatc gccagcgcgt
     6961 tcatgatggt ggccagcatg cccatatagt cggcctgggc ccggtccatg ccgcgggccg
     7021 cgcccgccat gccgcgaaag atgttgccgc cgccgatcac caggcagatt tccagccccg
     7081 tatcgcgcgc cgctttcact tccttggcca gttccgccac aaaggccgga tcgatgccga
     7141 actgctgatc gcccatcagc acctcgcccg acagtttgag caggatgcgt ttgtatgtcg
     7201 gcagctgcat gagacccctt atgcgcttga tgttgatcgg ccacagaaag gcccggcgca
     7261 ccagcgcccg ccccttccgg cgcgccctta tacccgcgcc gccgcccctg ccaagggcgg
     7321 caggtcaaaa agccggcgca aaatgccggc cgcgccgcgc caaacagcgc cccgggcggg
     7381 caaaaaccgc cggcctgtcg caaaacgaca agcgccgctt ccacccatgg cggaagcggc
     7441 gctgcgcaag ggccggcaaa ggcccccgtg tcaggcagaa atcaggcgag gccggccgcg
     7501 gccgccactt cggcagcgaa gtccgaagct tccttctcga tgccctcgcc cagctggaag
     7561 cggacatagt ccaccagcgc gatcttggca ccggcggcct tgccggcggc gtccaccacc
     7621 tgctgcacgg tgttcttgtt gtccatcacg aacagctgcg acaggagagc gttctccttg
     7681 gcatacttgg ccacggcgcc atccaccatc ttggcctgca cttcggcggg cttgccgctt
     7741 tcggcggcct tttcggcggc gatcttgcgc tcgcgctcga tcagctcggc gtcgaggtcg
     7801 tcagcgttca gcgccagcgg gaaagcggcg gcaatgtgca tggcgatctg cttgcccagc
     7861 ggctccagca cgtcggcgcc ggcttccgat tccagcgcga ccagcacgcc gatcttgccg
     7921 aggttggggg cagcggcatt gtgcatgtag ggcacgacca cgccggcctt gacctgcacc
     7981 gccttgatgc ggcggatctg ctggttctcg ccaatggtgg caacgttgct ggtcagcttc
     8041 tcgcccaccg tgccgccatc gggataggcc gccgccttca gcgcttccac atcgctgatc
     8101 gggccgccca gggccacttc ggtggccttg cgcacgaaat cctggaactg gtcattcttg
     8161 gcgacgaaat ccgtctccga gttcacttcc acagcgatgc ccgccgtgcc ggccacagcc
     8221 acgcccacca gaccttcagc ggcggtgcgg ctcgacttct tggcggcagc agccagaccc
     8281 ttggcgcgca gggcgtcgac ggcggcctcg aagtcgccac cggtctcgtc cagcgccttc
     8341 ttgcagtcca tcatgccggc gccagtgcgc tcgcgcaggt tcttcacgtc ggcggcagtg
     8401 taagccatgt ccgatttccc tttagtaagt aggcggtttc tacgtgctga cgccgggcca
     8461 cgtggacccg gcgccttgca tttttatgac gatccctctg gacgccccct cagggcgcgc
     8521 cccgggggaa cgcgcaggaa ggctcagccc tcgatggctt cttcagccag cgggttctcg
     8581 aaggcaccga tgtctgcgcc cgaatccacc accgcatcgc ggccaccctt ggtggcagca
     8641 gaggccacgg cctcgcagta gaggcggatg gcgcggctgg cgtcgtcgtt ggcggggatc
     8701 gggaaagcga tgccctcggg gctcacgttc gaatcgagga tggcgacgac cgggataccc
     8761 agcacgttgg cttccttgat cgccagctct tccttgttgg cgtcgatcac gaacatcacg
     8821 tcggggatgc cgcccatgtc gcggatgccg cccagcgaca gctcgagctt gtcgcgctcg
     8881 cgggtcatct ggaggacttc cttcttggtg aggccggcag tgtcacccga cagcttctct
     8941 tccagcgcct tgaagcgctt gatcgactgg ctgatggtct tccagttggt gagcatgccg
     9001 cccagccagc ggtggttgac gaagtgctgg ccgcaagcgc gggcggcctg ggcgatcggc
     9061 tcctgggcct ggcgcttggt gcccacgaac agcaccttgc cgcccgcctg cacggtggcc
     9121 gacacgaaat cgagcgcgcg cgcgaacagc ggcacggtct gcgacaggtc gaggatgtgc
     9181 acaccgttgc gggcgccgaa gatatacggc ttcatccgcg ggttccagcg gtgggtctgg
     9241 tggccgaagt gcgcgccggc ctcgatgagc tgctgcatgg agacgacagg agccgccata
     9301 gaaaattcct ttccggttac gcctctggga agcaggaacc cgctcttcgc gagggggcac
     9361 cggtctatgt cgcttcccat gtggggtttg gtcacatgat gccgggggcg gcgcgcgggt
     9421 tcccgcgttc cgattccgac gcggcggcgc ttacgcgcat tgcccggcaa aatccagccc
     9481 cgaaatgcgc agggcggccg gcatgcggca aaacctccac ggacgagcaa tcgccgcttg
     9541 acatgagcga acgttatgag aacatatagg ggccaaatgg aacagaccgg aacataggcc
     9601 ggcctttccc cggatacgga tatcgagcct gccgagtgcc tgttgaaacg cccgctgccc
     9661 gcgcagccgt tgaggaagga acagacatgc ccatctcgcc tgccgccgcc atgcacagcc
     9721 tgtccgtccc tggccttgcg gccgatgatc tgcgcacatg ggtagcgccc atgctggcga
     9781 tggtggcact gctggcgatc agcgtgctgg gggccagcct gatgaaggcg gcgcggctgt
     9841 tcgctgtgct gcgggaagaa gcgctggcgg ccggggccga acggccgatc gccctggcct
     9901 tgcggccggc ggcgatgcct gctgcctggc aggacagcga gatgccggct gtctggcagg
     9961 ccagccagat ggccgctgcc tggcaggcgg gccctgtggc cgcctgccgc gaggaatggg
    10021 tcaatcggcc gatgcgccag gcgctgcgcc ctgcaccgca gcagactggc gcgatgcccg
    10081 ctgccgcttg acccggccat aaacgacaag gccaatgggc agcagcgcga tataggcaat
    10141 gcacaggccc gccagcgtca gccagggttc gctaaacagt gcggcgaaga cgaggccgaa
    10201 caaggcgatc agttccagcc gcacggcccg gcgcgggcgc agcgcccccc agccaaaagt
    10261 ggcaaggctg gagatcatca gaaaagcgat caccaccagc cagatgccca ccacttgcgg
    10321 ctggcggaac agcggattgt cgctggagat ccagagatag agcggcagca tacccagggc
    10381 agcgcccatc ggcgccggca cgccggtgag gaaaccggcg gatttgtggg gctgctcggg
    10441 catgtcgatg cgggcgttga agcgcgcaag gcgcagcacg cagcacagcg cgaaggccag
    10501 agcggcaaac cagccaaggc gcggcgcctg ctgcaacgac cacaggaaga ggatgagcgc
    10561 cggcgccacg ccgaaggaaa tggcatccga caggctgtcc atctcggcgc cgaagcggga
    10621 ttgcgctttc agcaggcggg cgatgcggcc gtcgatcccg tcgagcacgc cggcgaggat
    10681 taccgccaat acggattttt cccaatcgcc ctgaatggca aagcggatgg ccgtgagccc
    10741 gcagcacagc gccgaggaag tgatcgcgtt ggggatcagc gcgcgcaggg tcaatccgcc
    10801 ggggaagcgg cccgccgccc cctcgcgcga gaagcccgca cgcgaaggcc ggctcattgc
    10861 gagatgccct cgatcagcgg ctggctgccc atggtggcaa ggatcgtctc gccggccacg
    10921 atgcgctggc caaggatgat cgccggttcc gtcccctcgg gcaggtagac atcgacgcgg
    10981 ctgccgaagc ggatcagccc cacgcgctgg ccggaggcga tgatgtcgcc cggcttcacg
    11041 aaaggcacga tgcgccgcgc caccaggccg gcgatctgcg tgaagccgat cagcacgccc
    11101 tgcccgcgat cgatcagaat gtgctggcgc tcgttttcct cgctcgcctt gtcgagatcg
    11161 gcgttgagga agcggcccgg aatatagacc acgcggcgca ccgtgccgcc aatgggcgcg
    11221 cggttgatgt gcacatcgaa cacgctcatg aagatggaaa tgcgcgtgac cggctgggtg
    11281 ccaaggcacg gcaggccgct gccatcatcg gtcgacagtt cgcgcggggg cggcactttc
    11341 tggatcagcg tgaccagccc atcggccggc gcgacgatga agccctcgcc gcgcggggtg
    11401 acccgctgcg gatcgcggaa gaaggcgcag atgcaatagg tcaggatccc cagcggccag
    11461 gccagcaggg agaggccgaa gatcgccgcc aacaggcagc cgatcgcggc gacagcggca
    11521 aacttgcgcg cttcgggatg gacggagggc caatgccaac tggcagcacc atggccccgg
    11581 ttatcaagaa tttcaccagg catgtgcgct ctctagggcc tgccctgccg gtgggcaagg
    11641 cgtgatcgtt ggcaggaagg aggccgcgac cgccttcccc ttgcgggata ttgttgatcc
    11701 tggaaggaga aagcggtggt ggacagggct agattcgaac tagcgtacgc ttgcgcgggc
    11761 agatttacag tctgctgcct ttaaccactc ggccacctgt ccacaccgtg cttgccagtg
    11821 ccggaacggt gaaagccgtt ccatttgaag aacctgattg ctcaggtccg ttcggccgag
    11881 gagcgcgcta atggccaaag cggccttgcc tgtcaatggg gctttgtgca tggggcgcaa
    11941 gaaattttcg agaaaggcaa aagcatgggc aaaaatggcg aagaaggcgg tggccgccgc
    12001 gagggcaggt tgctgcgcgg gcgcgcgggc cgcatgcagg gcgggcgcgg ctctggccgg
    12061 ggcagcaagg gcgcggtgcg cctgtggggc cgccacgcgg tggaagcggc gctgaagaac
    12121 cccgagcggc gccaccgcaa gctgtgggcc acgcgggagg gcattgccag cctggatggc
    12181 gaattgccgg ctgatttccc gctggaatat gcgcaggcgg ccgatctggg ccggctggtg
    12241 gccaaggatg cgccgcatca ggggctggtg ctggattgcg agccgctgga ggatatctat
    12301 ctcgacgacg tgctctcggg cgatccggcg cggccgctgg tggtgctcga ccaggtgacc
    12361 gatccgcata atgtcggcgc catcctgcgc tcggcagccg cgttcgatgc ctgcgccatc
    12421 gtcacgcagg atcgccattc gccgcccgaa tcgggcacgg tggccaagtc tgcctcgggc
    12481 gcgctggaaa tcgtgccgtg ggtgcgcgtg gtgaacctgg cccgcgcgct ggaggagatt
    12541 gccgaggccg gctattggcg aatcggcctt gccggcgaag cgcgctccac cctggcagcc
    12601 gccctgcccg caggccccgt ggcactggtg ctgggcgccg agggcgaagg catgcgccac
    12661 aacatcaccc agcattgcga cgcgctggcc cgcctgccga tcagttcagc aatggaaagc
    12721 ctcaacgtct ccaatgcggc ggcaattgcg ctttatgcgg cggcctcgcg cgccgatatt
    12781 gctgaggagg actgaaccgg cacggcaggg ggcagccagg cgccccctgc cccggcgccg
    12841 cagtgcgcac gccaaaacaa aaccgcgccc caccccaagc cggggcagag cgcggttttc
    12901 tgtcaggaag cggcggcggc aggggccagc cggcaacccg tcaaccaatc agttgacggc
    12961 gtccttcagg cccttgccgg ccttgaactt gggctgggcc gaagcagcaa tcggcatcgg
    13021 ctcaccggtg cgggggttgc gcccggtcga cgccttgcgc ttggcgaccg agaaagtgcc
    13081 aaaaccaacc aggcgcactt catcgccatt cttcagcgcc gacgtgatcg catcgaacac
    13141 gccttccacc gccttggtgg cgtcattgcg ggtgaggcca ctggcttccg caaccgcgcc
    13201 gatcagatcg ttcttgttca ttcgggaacc ccctaccttt caatggaccg ggtaactttc
    13261 tgaatcaccc ggggaagagg cgcggaattg agcggttttc gatggttctg tcaaagccaa
    13321 aagcgcgctt ttgcgcccca tttaccctaa tccaggctta atttcagggc tgacaaagcc
    13381 ggaaaacggc aaaatcatgc ctgcgttaac ggtgccgcgc ggggcccacc ccgattcgcg
    13441 gcaccgcagc agaatcgcct tgttcagtgc gccgtggccg ccgggctgcc gggcgccggc
    13501 aacgcaccag gctggctcgc cagatcatcg gcctcggtcc attcgatcgg cgccaccggc
    13561 tgggccagcg cgcgcgccag cacctcgtcc acatgggcca ccgcgatgat ctccagcccg
    13621 gccttgatat tggccgggat ctcggcgaga tccttctggt tctcctcggg gatcagcacc
    13681 gtcttgatgc cgccgcgcag ggccgccagc agcttttcct tcagcccgcc gatcgccagc
    13741 acccggccgc gcagcgtcac ctcgccggtc atcgccacat cggcgcgcac gggaatgccg
    13801 ctcagcgtcg agaccatcgc cgtcaccatg ccgatgccgg cgctggggcc atccttgggc
    13861 accgcgccct cgggcaggtg gatgtggatg tccttgcggt taaagatcga gggcttgatg
    13921 ccataggccg gcgcgcgcgc cttcacgaag ctgaaggccg cctgcacgct ctcggtcatc
    13981 acctcgccca gcttgccggt ggtcttgatc gcgcccttgc cgggcacggt cacgctttcg
    14041 atggtcagca gttcgccgcc cacttcggtc caggcgaggc ccgtcaccgc accgatctgg
    14101 ttctcatcat ccgacatgcc atggcggaac ttgcgcaccc cggcaaagtc ggccagattt
    14161 tccggcgtga taaccacacc ggtttccttg ccttccagaa tgcggcgcag gctcttgcgc
    14221 gccagccggg caatctcgcg gtccagcgtg cgcacgcccg cctcgcgggt gtagtagcgg
    14281 atcagatcgc gcaaggcagc agaggtcagc tcgaactcgc ccttcttcag cccatgctgc
    14341 tgcacctggc gcgggatcag atggcgctcg gcgatctcca gcttctcatc ctcggtatag
    14401 ccctccagcc ggatgatctc catccggtcc agcagcggct ggggcaggtt gaggctgttg
    14461 gccgtgcaca cgaacatcac gtccgacagg tcgatgtcca gctccagata atgatcctgg
    14521 aacttggcgt tctgttccgg atccagcacc tccagcagcg ccgaggccgg atcgccgcgg
    14581 aaatcctggc ccagcttgtc gatctcatcc agcaggaaca gcggattgga cgtgcccgcc
    14641 ttcttcaggt tcgagacgat cttgcccggc atcgagccga tataggtgcg ccggtggccg
    14701 cggatctcgg cctcgtcgcg cacgccgccc agcgactggc ggatgaactc gcgccccgtc
    14761 gcccgcgcga tcgatttgcc aagcgaggtc ttgcccacgc ccggcgggcc gacgaggcac
    14821 aggatcggcc ccttcagctt gttcgtgcgc gcctgcaccg ccagatattc aacgatgcgg
    14881 tccttcacct tgtccagcgc gtaatggtcg gcatcaagga tcgcctgcgc cgcgccaatg
    14941 tccttcttca gcttgctctt cttgccccag ggcaggccca gcagcacatc gagatagttg
    15001 cgcacaacgg tcgcctcggc gctcatcggc tgcatcgtct tcagcttctt cacctcggcc
    15061 tcggccttgg cccgcgcttc cttggagagc ttgagcttgg cgatcttctc ggtcagctcg
    15121 gcaatctcgt tcgcctcgcc atcatcgccg ccgcccagct cgctctggat cgccttcaac
    15181 tgctcgttga ggtaatattc gcgctgggtc ttttccatct ggcgcttcac gcggccacgg
    15241 atgcgccgct ccacctgcag cacgcccagc tcgccttcca tcaggccata gaccatctcc
    15301 agccgcttca gcggatcgat ctcggagagc agcgcctgct tgtccgagac cttggcgctg
    15361 atatgggcgg ccaccgcatc ggccagcttc gaggcttcct cgatatcgcc cagctgatcg
    15421 ccggcttcct ggctgagctt cttgttcagc ttggcatatt cgtggaactg atcgaccacg
    15481 ctgcgcatca tcgcggccac ttccgggcct tcggcggttt ccggctcgat atcggcgaca
    15541 tcggccatca gcagcgtggg ctcttccttc agcccggcca gcttggcgcg gctctgccct
    15601 tccaccagca cgcgcacggt gccatcgggc agcttcagca gctgcaacac gctggccacc
    15661 acgcccacgt catagaggtc gtcggcctgg gggtcgtcgc agccgggatc aagctgggcg
    15721 aggaggaaga tcgtcttgtt gccggccatc gccgcttcca gcgccgccac cgatttctcg
    15781 cggccaacga acagcggcac caccatgccc ggaaagacga caatgtcgcg cagcggcaac
    15841 aggggaagag aggtcacgtc catatccatc catcatagcg cgcaaagcgc atttccggcc
    15901 atggcggggc atttaaaagc ccatgccgct accggcgtct gaagggaata tggggggcgc
    15961 ccggctcggt tgcaatgggc gcaaatagca ggcttgtgga ttggcgctgc ccgcagcggc
    16021 cggtgccgcc gcaggcaggc gcccgcaagc cacgttcaga acggcagctt gaagcccggc
    16081 ggcaggccca tgcccgcctg cgccttggcc agctcggcgc cggccacttc atcagccttg
    16141 gcgcgcgcat cgttgaaggc ggcggccacc agatcttcca gaatgccctt ttccgagggc
    16201 gccagcaggc tgtcgtcgat ggcaacgccg atcacccggc ccttggcact ggcgcggatc
    16261 ctgaccaggc cgccaccggc cacgccttcc acttccagcg aatccagccg cccctgcgcc
    16321 tcgttcatct gcttgctgat cgtctcggcg gcctgctggg ccgcagcgat catctcttcc
    16381 atggatttca tctagtttct ccaattggcc tgacgtgggc gctggggggc gcccggctcg
    16441 tcacggataa tctcggcagc gggaaaggcg gccagggcgg cggccaccag cggcgcggcg
    16501 cgctcggcct cgccgcgggc gcgcacatcg gcgcgatggg cctcatccag cgtgggcgcg
    16561 ccgccttcgg cctggcgctc cacatgccac agctgcccgc tcaccttctg cagcatggcg
    16621 cgcatctcgg ggccaaggtc gtcgcgaaat tccggcggct gggcaaacac gaggcgccct
    16681 tgcgccagct cgatcacccg cacctgcacg cgcatcatgc tggccatggt catgccgccg
    16741 ggcaggcccg attcctccac ctgctccacc agcgcgtccc agctcagcgc gggcgccagc
    16801 gcctcggcgc cgccgccggg cagggcgtcg ggcccgggcc cgcctgccgc cgccagcacc
    16861 gcgcccgatg ccgccatctg ctcgatccgc cgggccagct ggccaggatc gggcagatcc
    16921 gccgcatgca ggcagcgcaa cagcgccatt tcggccgcca ccagcgggtc tggcgcgccc
    16981 ttcacttcct cgtgcccctt cagcagcaat tgccacagcc ggtgcacctg cccggccgac
    17041 aggcgcttgc cccaatcctc gatcgcggcg cgttcctcgt cagacacggc gcgcacatcg
    17101 ccccggccca gctgcgccac cgccacccgg tgcgccagct ccatggcgcc gcgcatcagc
    17161 gccagcggct ccacccccag cgcatattgg ttggccacca gatcgagcag atccgccccc
    17221 tcgcccgcca gcagcgcggc aaacaggcgc ctttgcgcgc ccttgtcggc caggcccagc
    17281 atgtcgcgca cctgctcggc cgtcaccagc gtggcagcgc cggcggcggc catatcggca
    17341 tgggcaatcg cctgatcgag gatcgacagc ccatcgcgca cggagccttc ggcggcctgt
    17401 gccaccatcg ccagcgccgg cgcttctgcc tccacccctt cggccgcgca aatgccggcg
    17461 aaatgcgtcg ccagcagcgg cgccgggatg cgcttcagat cgaaacgctg gcagcgcgaa
    17521 agcacggtga tcggcagctt gtccacctcg gtggtggcaa acaggaattt cacatgcgcc
    17581 ggcggctctt ccagcgtctt cagcagcgcg ttgaaagcat tgcggctgag catgtgcact
    17641 tcgtcgatga tatagatctt gtagcgcgcc gacaccgccg catagcgcac cgcctcgatg
    17701 atctcgcgca catcatcgac gccggtgtgg ctggccgcgt ccatctcgat cacgtcgata
    17761 tggcggccct cggcaatcgc ccggcaaggc tcgcactggc cgcaagggtc gatcgtcggc
    17821 ccgccctggc catcggcgcc cacacagttc agcgccttgg cgataagccg cgcggtgctg
    17881 gtcttgccca cgccgcgcac gccggtcatc aaaaaggcat gggccagccg gtcgcgcttg
    17941 atggcattgg ccagcgtctg caccatggca tcctggccga tcagcgcgga aaaggtttgc
    18001 gggcgatatt tgcgcgccag cacgcgatag ggctggctgg ccgccggttt ggccacaacc
    18061 gggggcgcgg cctgtgcggc aggcgccggc ccgccaaaca gcgcggattg ccccgccgct
    18121 tccagttcgg ctgcggtggg cagtgcctct tgcgcctgcc ctgcctcggt ggaggctgta
    18181 tgttgtggtg aatcgcccat catcggcccg agtggtaagg gcagatgccc gaaatgtcga
    18241 agggctttac gccgccggcg cgattatcaa ccatcgcgcc cgctggcgtt tgaaggagcc
    18301 gggcgacccg ccgcaactcg ttgtggctgc ttccttccgg acctgaccag gttggcgaac
    18361 gctaacgtcc acccgactcc cggcccggca tatggcgggg cttggccgtc agcgcaagcc
    18421 cctgcggcaa gaaagatcag cgaaaattgt cggcataggc ctgcagcttc agccggcgcg
    18481 gcggcgtggc gtgcacggca gcggcaatgc catacttgtc ggcataggcc tgggcctcgc
    18541 cctgggtgtg aaacttcagc tgcacctggc tttgcgtgtc gccgctgccc gcccaaccca
    18601 tcaacgggtc ggcatggcgc gcttcggcgg gcgcaaattc gaggatccac tggccaagca
    18661 gcgccttacc ggactgcatg gcatttttcg ggcgctggta gatgcgtgcg gtcatgccat
    18721 tgcccttgcc agcgaatcgc cgccaaaatc aagtgcgacg ttgggctgaa aaggcgtttt
    18781 tcgtcttcag gctcgggtct tcggcccagg gctcgtcggg ccagcggtgg cggggatagc
    18841 ggcccttcat ttccttcacc acatcgcgcc agctgccgcg ccagaaaccg ggcagatcgc
    18901 gcgtggtctg gatgggcttg ccgccgggcg aagtgagctt cagcagcagc ggctgggcgg
    18961 gctggccaaa gcccgggtgc cgctccagcc cgaacagggc ctgcacccgc acttccacgc
    19021 tgggcccgcc gggatcgtcg taatcgatgg agtggctggt gccggcgggc gagcggaatt
    19081 cggccggggc cagccgctcg agcgcctgct tgccctccca gccca
//
LOCUS       DASRYE010000253         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_227285,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000253 DASRYE010000000
VERSION     DASRYE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227285"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..815)
                     /gene="ubiG"
                     /locus_tag="VFF98_12855"
     CDS             complement(66..815)
                     /gene="ubiG"
                     /locus_tag="VFF98_12855"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HZU64564.1"
                     /translation="MSIATVNDTSGPATIRPEEAAHFGKLAADWWDPKGSSAMLHKLN
                     PVRLGFVRRAIDGHWPAESQGLKPLAGRRALDVGCGAGLLAEPLARLGAAVTAVDAAA
                     ENIAAARAHATAMGLAIDYRHGEIGALGLGQFDLVTSMEVIEHVADKRAFLAALVACL
                     APGGLLILSTPNRTASSRLLMVEGAERLGLIPRGTHHWEDFIQPAELALMLEELGFAP
                     GNPMGIAWSPMKGLHLSEDLSLNYILSARRI"
     gene            902..2155
                     /locus_tag="VFF98_12860"
     CDS             902..2155
                     /locus_tag="VFF98_12860"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZU64565.1"
                     /translation="MARIVMKFGGTSMAGSERIRRVAGIVKRQQEAGHEVAVVVSAMA
                     GETDRLVGFCREAQTLYDPAEYDVVVASGEQVTSGLLAMTLQSLGSKARSWLGWQMPI
                     HTDNAYSKARIEGIDTDALLASMAAGEIAVIPGFQGLSPEGRVTTLGRGGSDTSAVAV
                     AAALKADRCDIYTDVDGVYTTDPRIVAKARKLKNVTYEEMLELASVGSKVLQTRSVSL
                     AMKEKVRVQVLSSFIDENAPAADTLPGTMIVSDEELEGLDMERQLITGIAADKNDAKV
                     ILTAVPDRPGAVSAIFGPLAEANINVDMIIQNVGRADTDTDVTFTVPQADLDRTVAVL
                     EANRDHIRYEKLSTDPKVAKISVVGVGMRSHAGVAATMFKALADRGINIQAISTSEIK
                     VSVLIDADEVELAVRLLHTAYGLDG"
     gene            2287..3267
                     /locus_tag="VFF98_12865"
     CDS             2287..3267
                     /locus_tag="VFF98_12865"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZU64566.1"
                     /translation="MARGAAFLGSETAILCGAMSWVSERNLVSAISNAGGFGVIACGA
                     MSPELLDAEIAATRALTARPFGVNLITMHPQLFDLIAVCARHGVSHVVLAGGLPPKGS
                     VEAIKETGARVICFAPTLALGKKLLKSGADALVIEGNEAGGHIGPVSTSVLAQEILPA
                     LAADHLVFVAGGIGRGEMIASYLEMGAAGVQLGTRFAAAVESIAHADFKKAFFRASAR
                     EAVASVQIDPRLPVIPVRALKNKGTEAFTAKQVEVAGKLNAGEVDMHEAQLLIEHYWA
                     GALRRAVIDGDVEGGSVMAGQSVGMVTREEPVAEIIASLLGECEDALARR"
     gene            3354..4226
                     /locus_tag="VFF98_12870"
     CDS             3354..4226
                     /locus_tag="VFF98_12870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZU64567.1"
                     /translation="MCDEWTSAGEEAALAGAGLSRRAFAASAAAAVATASAASQAAAA
                     EAGLIETPVLIPTGDGKADGFFIRPAHGTHPAILMWPDIAGLREAYKVMARRLALAGY
                     AVLVVNHYYRTARAPLLASMAEWMTPAGQARLKPAIAAITPARTLADARAFIGFLDAQ
                     PGVARGRGIGTCGYCMGGPYAVRTAAAVPARVAAVATLHGAGLVTDAPDSPHRLLGET
                     KAAFLFAIARNDDARAPADKDALKAAAAAAHRPAEVEVYAADHGWCTIDAPSYNKAEA
                     ERAWGRMLSLFSRL"
     gene            4291..4692
                     /gene="msrB"
                     /locus_tag="VFF98_12875"
     CDS             4291..4692
                     /gene="msrB"
                     /locus_tag="VFF98_12875"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZU64568.1"
                     /translation="MTDRIAITDAEWREKLTPMQYHVLREGGTERAFTGAYEPNKAAG
                     DYVCAGCGAPLFVSGTKYNSGSGWPSYYAPISPEAVDEHRDISHGMVRTEVRCARCDG
                     HLGHVFPDGPQPTGLRYCINSAALDFHPKEP"
     gene            4806..>6977
                     /locus_tag="VFF98_12880"
     CDS             4806..>6977
                     /locus_tag="VFF98_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZU64569.1"
                     /translation="MAQGKGGRQSQGRGGGKPGGGGGFWRRLLFWLAMPVLALAIVVA
                     AIVGAEAQSLPSFDSMKSSQVGQTIIVRARDGTPLVSLGPSYGKWLSYDELPEVMKQA
                     IVSTEDRRFREHIGIDPIGIVRSLFVRAQSGHWRQGGSTITQQLARTVFLNNNRTFGR
                     KAREALLAMALEWKFSKDQILELYLNKVYFGGGAYGVDAASRKFFGHAGTQLSLPEAA
                     IIAGLVKAPSHYSPTADAQAAVDRASVVLDVMQDAGAISAEQHANADLKAVKMAPEAA
                     QNSVRYFTDWALPQLDQILPDNQQPIEVWTTLDPRMQAAATSAIQANVPRDKKGGGLA
                     QGALVSLDRDGAVLAMVGGTDYVTSNYNRATMAMRQPGSAWKLFVYLAALEAGVKPDD
                     PVVDQPVTIDGWTPHNDAGRYAGQIDVRTAFAYSKNTVAAQLGQQVGFTTVADMAKRF
                     GITSPITTNPSMVLGTNEVRLIEMARAFAEVSARGTSVEPYGITKVVTTDGRVLYQRP
                     PNQGQTLVAPFVAAGITDLLQTAVNIGTGRAAQIGRPVAGKTGTTSSNKDGWFMGFSS
                     GITTGVWMGRDDAKPIPGLMGGTAPARAFSQYMRVAVAGRPVEQFDTQLKLPEWQLEP
                     DDESYLNNPGSYYYTDDQGNLVEGAGNAAAGQQQQQQQGDGTQPADQPRAPVSQPRAP
                     VGRPASQPLFAPGEQGGAPAAANQDFLDQATGSRSRANRRVP"
BASE COUNT         1159 a         2320 c         2437 g         1061 t
ORIGIN      
        1 acccggccac ccaacgatag taccctaggg gtggccgggt gggggagagg gccggtgccg
       61 ggccgttaaa tccgacgcgc cgagaggatg tagttcagcg agaggtcttc gctgaggtgc
      121 aggcctttca tcgggctcca ggcgatgccc atcgggttgc ccggggcaaa gcccagttcc
      181 tccagcatca gcgccagttc ggccggctgg atgaaatcct cccagtgatg cgtgccgcgc
      241 gggatcaggc ccagccgttc ggccccttcc accatcagca ggcgcgaact ggccgtgcgg
      301 ttgggggtgg aaaggataag caggccgccg ggcgcgaggc acgccaccag cgcggccagg
      361 aaggcgcgct tgtcggccac atgctcgatc acttccatgc tggtcacaag gtcgaactgg
      421 ccaaggccca gcgcgccgat ctcgccatgg cgataatcta tcgccaggcc catcgctgtg
      481 gcatgggcgc gcgctgcggc aatgttctcg gccgcggcat ccaccgcggt aacggccgcg
      541 cccagccggg ccagcggctc tgccagcagg cccgccccgc agcccacatc cagcgcgcgc
      601 cggccagcca gcggcttcaa cccctgcgac tcggccggcc aatgcccgtc gatcgcccgg
      661 cgcacgaagc cgaggcgcac cgggttcagc ttgtgcagca tggccgagct gcccttggga
      721 tcccaccagt ctgcggccag cttgccgaaa tgggcggctt cctcggggcg aatggtggcc
      781 gggccactgg tatcgttcac ggttgcaatg ctcatcacag cctcctaaag cgccccgtcc
      841 gcccggggca aggcccttgg gcgcctttat cgttgatgct agcagattgg gagccccaga
      901 cgtggcccgc attgtgatga aattcggcgg cacctccatg gccggcagcg agcggatccg
      961 gcgcgtggcc ggcatcgtca agcgccagca ggaagccggc cacgaagtgg cggtggtggt
     1021 ttcggccatg gccggcgaga ccgaccggct ggtgggcttc tgccgcgagg cgcagacgct
     1081 gtatgacccg gccgaatatg acgtggtggt ggccagcggc gagcaggtga cgagcggtct
     1141 gctcgccatg acgctgcaat cgctgggcag caaggcgcgc agctggctgg gctggcagat
     1201 gccgatccac accgacaatg cctattccaa ggcccgcatc gaggggatcg acaccgatgc
     1261 cctgctggcc agcatggccg ccggcgagat cgccgtgatc cccggcttcc agggcctgag
     1321 ccccgagggc cgggtgacga cgctgggccg cggcggttcg gatacgtcgg cggtggcggt
     1381 ggctgccgcg ctgaaggcgg atcgctgcga catctacacc gatgtcgatg gcgtctacac
     1441 caccgatccg cgcatcgtgg ccaaggcgcg caagctgaag aacgtcacct atgaggaaat
     1501 gctggaactg gcgagcgttg gctcgaaagt gctgcagacc cgctcggtga gcctggccat
     1561 gaaggagaag gtgcgcgtgc aggtgctctc ctccttcatc gacgagaacg cgcccgccgc
     1621 cgacacgctg cccggcacga tgattgtttc tgacgaagaa ctcgaaggac tggatatgga
     1681 acgccagctg atcaccggca tcgccgccga caagaacgac gccaaggtca tcctcaccgc
     1741 cgtgcccgac cggccgggcg ctgtgtcggc gatcttcggc ccgctggccg aggcgaacat
     1801 caacgtcgac atgattatcc agaatgtcgg ccgcgccgat acggataccg acgtgacctt
     1861 caccgtgccc caggccgatc ttgatcgcac cgtggccgtg ctggaagcca accgcgacca
     1921 tatccgctac gagaagctgt cgaccgaccc caaggtggcc aagatctcgg tggtgggcgt
     1981 gggcatgcgc agccatgccg gcgttgccgc caccatgttc aaggcgctgg ccgatcgcgg
     2041 catcaacatc caggcgatct ccaccagcga gatcaaggtc tcggtgctga tcgatgccga
     2101 cgaggtggaa ctggccgtgc gcctgctgca caccgcctat ggccttgatg gctgattgag
     2161 cggcgcgaag gcggcttctc gccccttcgc gccacctccg gctgaggccg gccataaaac
     2221 cgcaagcctt caggcgagga cagcttagtg aacgagagca gcgtaaccac ccgcacggcc
     2281 gccctgatgg cgcgcggcgc ggcgtttctg ggcagcgaga cggcgatcct gtgcggcgcg
     2341 atgagctggg tttccgagcg gaacctggtg agcgcgatca gcaatgccgg cggctttggc
     2401 gtgatcgcct gcggggcgat gagccctgag ctgctggacg ccgagatcgc cgcgaccagg
     2461 gcgctgaccg ccaggccctt cggcgtcaac ctcatcacca tgcacccgca gctgttcgat
     2521 ctgatcgccg tttgcgccag gcatggggtc agccatgtgg tgctggccgg cggcctgccg
     2581 cccaagggca gcgtggaggc gatcaaggaa accggcgcgc gggtgatctg ctttgccccc
     2641 acgctggcgc tgggcaagaa gctgctgaag agcggcgccg atgcgctggt gatcgagggc
     2701 aatgaggcgg gcgggcatat cggccctgtc tccaccagcg tgctggcgca ggagatcctg
     2761 ccggcgctgg cggccgatca tctggtgttt gtggccggcg gcatcggccg gggcgagatg
     2821 atcgcttcct atctggaaat gggggcggcc ggcgtgcagc tgggcacgcg ttttgccgcc
     2881 gcggtcgaat cgatcgccca tgccgatttc aagaaggcct tcttccgcgc cagcgcgcgc
     2941 gaggccgtgg ccagcgtgca gatcgatccg cgcctgccgg tgatcccggt gcgcgcgctg
     3001 aagaacaagg gcaccgaggc cttcaccgcc aagcaggtgg aagtggccgg caagctgaat
     3061 gccggcgagg tggacatgca cgaggcgcag ctgctgatcg agcattactg ggctggcgcg
     3121 ctgcgccgcg cggtgatcga tggcgatgtg gaaggcggct cggtgatggc cggccagtcg
     3181 gtcggcatgg tgacgcgcga agagccggtg gccgagatca tcgccagcct gctgggcgaa
     3241 tgcgaggacg cgctcgcccg ccgctgatct gatacgcgct gatacagggc tcccggttgc
     3301 cccgcctgcc ggccctgtgc cacaagggcg ggcaggccat tgggagacag gcgatgtgcg
     3361 atgaatggac gagcgcgggc gaagaggccg cgctggccgg ggcggggctc agccgccggg
     3421 cctttgcggc cagcgccgcg gccgccgttg ccactgccag cgccgccagc caggccgcag
     3481 ccgccgaggc cgggctgatc gagacaccgg tgctgatccc caccggcgat ggcaaggccg
     3541 atggtttctt catccgcccg gcccatggca cccaccccgc gatcctgatg tggcccgata
     3601 ttgcgggcct gcgcgaggcc tataaggtga tggcgcggcg gctggcgctg gcaggctatg
     3661 ccgtgctggt ggtcaaccac tattaccgca ccgcccgcgc gccgctgctg gccagcatgg
     3721 ccgaatggat gacgccggcc ggccaggcca ggctgaagcc ggccatcgcc gccatcaccc
     3781 cggcgcgcac gctggctgat gcgcgcgcct tcatcggttt tctcgatgcc cagccgggcg
     3841 tggccagggg gcggggcatc ggcacttgcg gctattgcat gggcgggccc tatgcggtgc
     3901 gcacggcagc ggcggtgccg gcgcgggtgg cggcggtggc aacgctgcat ggcgccggcc
     3961 tggtgaccga cgcgcccgac agcccccatc gcctgctggg cgagacgaag gcggcattcc
     4021 tctttgccat cgcccgcaac gatgatgccc gcgcgccggc cgacaaggac gcgctgaagg
     4081 cggccgccgc tgccgcccat cgcccggccg aggtggaagt ctatgccgcc gatcatggct
     4141 ggtgcacgat cgatgcgccc agctacaaca aggccgaggc cgagcgggcc tgggggcgga
     4201 tgttgtctct tttcagcagg ctgtaaggac ttggcgccgg ccccggcgca ccccataatg
     4261 gatccagacc gtgaaggagg cccccccgcc atgaccgacc gaatcgccat caccgatgcc
     4321 gagtggcgcg aaaagctcac ccccatgcag tatcacgtgc tgcgcgaggg cggcaccgaa
     4381 cgcgccttca ccggcgccta tgagccaaac aaggcggcgg gcgattatgt ctgcgccggc
     4441 tgcggggcgc cgttgttcgt atccggcacg aaatataatt ccggctcggg ctggcccagc
     4501 tattatgccc ccatctcgcc cgaagcggtg gatgagcacc gcgacatctc gcatggcatg
     4561 gtgcgcaccg aagtgcgctg cgccaggtgc gacgggcatc tgggccatgt cttcccggat
     4621 gggccgcagc caacgggcct gcgctattgc atcaacagcg cggcgctcga ttttcacccc
     4681 aaagagccgt aaatcgcccc gggctggcgc ctttttgtga aatccgcggt taacccggcg
     4741 gcttctatcg cctaagggag cgggcggccg caaggcttgc acgacagcga ggacacacaa
     4801 ggcgcatggc acaaggcaag ggtgggcggc aatctcaggg gcgcggcggt ggcaagccgg
     4861 gcggcggggg cggcttctgg cgccggctgc tgttctggct ggcgatgccg gtgctggcgc
     4921 tggccatcgt ggtggccgcg atcgtcggcg ccgaggcgca gagcctgccc agcttcgaca
     4981 gcatgaaatc cagccaggtg ggccagacga tcatcgtgcg cgcgcgtgat ggcacgccgc
     5041 tggtgtcgct ggggcccagc tatggcaagt ggctctctta tgacgagctg cccgaggtga
     5101 tgaagcaggc catcgtttcc accgaggacc ggcgctttcg cgagcatatc ggcatcgatc
     5161 cgatcggcat cgtgcgctcg cttttcgtgc gcgcgcaaag cgggcactgg cgccagggcg
     5221 gctccaccat cacccagcag ctggcgcgca cggtgttcct caacaacaat cgcacctttg
     5281 gccgcaaggc gcgcgaggcg ctgctggcca tggcgctgga gtggaagttc tcgaaggacc
     5341 agatcctcga gctttatctc aacaaggtct atttcggcgg cggcgcctat ggcgtggatg
     5401 ccgccagccg caagttcttc ggccatgccg gcacccagct ttccctgccc gaggcggcga
     5461 tcatcgccgg gctggtgaag gcgcccagcc attattcgcc cactgccgat gcccaggccg
     5521 cggtggaccg cgccagcgtg gtgctggatg tgatgcagga tgccggcgcc atcagcgccg
     5581 agcagcacgc caatgccgac ctcaaggccg tgaagatggc gcccgaggcg gcgcagaatt
     5641 cggtgcgcta tttcaccgat tgggcgctgc cgcagctgga ccagatcctg cccgacaacc
     5701 agcagccgat cgaggtgtgg acgacgctgg accccaggat gcaggcggcc gccaccagtg
     5761 ccatccaggc caatgtgccg cgcgacaaga agggcggggg cctggcgcag ggcgcgctgg
     5821 tcagcctcga ccgggatggc gcggtgctgg cgatggtggg cggcacggat tacgtgacca
     5881 gcaactacaa ccgcgccacc atggccatgc gccagccggg ctcggcgtgg aagctgttcg
     5941 tctatctggc cgcgctggaa gcgggcgtga agccggatga tccggtggtg gaccagccgg
     6001 tgacgatcga cgggtggacg ccgcacaacg atgccggccg ctatgccggc cagatcgacg
     6061 tgcgcaccgc ctttgcctat tccaagaaca ccgtggccgc ccagctgggc cagcaggtgg
     6121 gcttcaccac cgtggccgac atggccaagc gctttggcat caccagcccg attaccacca
     6181 acccttccat ggtgctgggc accaatgagg tgcggctgat cgagatggcg cgcgcctttg
     6241 ccgaggtgtc ggccaggggc acctcggtgg agccctatgg catcaccaag gttgtcacta
     6301 ccgatgggcg ggtgctctat cagcgcccgc ccaaccaggg gcagacgctg gtggcgccct
     6361 ttgtggctgc cggcatcacc gatctgctgc agacggcggt gaacatcggc accggccgcg
     6421 cagcgcagat cggccgcccg gtggccggca agaccggcac cacctccagc aacaaggatg
     6481 gctggttcat gggcttttcc agcggcatca ccaccggcgt gtggatgggc cgcgacgatg
     6541 ccaagccgat ccccggcctg atgggcggca ccgcgcctgc ccgcgccttc tcgcaatata
     6601 tgcgcgtggc ggtggccggc cggccggtgg agcagttcga cacccagctg aaactgcccg
     6661 aatggcagct ggagccggat gacgagagct atctcaacaa tcccggcagc tattactaca
     6721 ccgacgatca gggcaatctg gtggaaggcg cgggcaatgc cgcggccggc cagcagcaac
     6781 agcagcagca gggcgatggc acccagccgg ccgaccagcc ccgggcgcct gtcagccagc
     6841 cgcgcgcgcc ggttggccgg ccggccagcc agccgctgtt cgcgccgggc gaacagggcg
     6901 gcgcgccggc cgcggcgaac caggatttcc tcgatcaggc aaccggcagc cgcagccgcg
     6961 ccaaccggcg ggtgccg
//
LOCUS       DASRYE010000254        15998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_167924,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000254 DASRYE010000000
VERSION     DASRYE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15998
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167924"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFF98_12885"
     CDS             complement(<1..593)
                     /locus_tag="VFF98_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HZU64570.1"
                     /translation="MTPAARLQAAIEILDAVIAAARAGGAPAERVVAEGLRARRYAGS
                     GDRRAIREHVFAAIRACGPVPASGRAALLRVADRALFDGSAHGPAPLAAGEQAADGGV
                     APLWLAQRLAASGLAAEAAEALLGRAALDIRVNAARADRAALALPVAGEALAFTRQGL
                     RLPAGTPVEQWEAYAQGLIEVQDAGSQLACGVVAARPG"
     gene            complement(665..2182)
                     /gene="guaB"
                     /locus_tag="VFF98_12890"
     CDS             complement(665..2182)
                     /gene="guaB"
                     /locus_tag="VFF98_12890"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZU64571.1"
                     /translation="MGRHKSASPIPSKRETSVAILDIPLGLTFDDVLLRPAESDILPS
                     MADTRTRLTREIALNIPVLSAAMDTVTEADMAIVMAQMGGIGVLHRNLTIEEQCAAVR
                     AVKRFESGMVVNPITISPHATLGEAQAIMQANRISGIPVVEGNGKLVGILTNRDVRFA
                     DNAAQPVRELMTHENLATVRAGVSQEEARKHLHQRRIEKLLVVDDAYHCVGLITVKDI
                     EKAVTYPNATKDGAGRLRVAAATTVGDKGFARTEALLEAECDVVIIDTAHGHNRDVAR
                     AVERVKKLSNAAQVVAGNVATAEATRALIEAGADAVKVGIGPGSICTTRIVAGVGVPQ
                     LTAIMESAEEATKHGVPVIGDGGLRTSGDAAKALAAGASTVMIGSLLAGTEEAPGETF
                     LYQGRAYKSYRGMGSVSAMARGSADRYFQQDIKDQMKLVPEGIEGQVPYKGPAKDVVH
                     QLVGGIKAAMGYTGSATIEHLRTKANFIRITNAGLKESHVHDVTITREAPNYPTR"
     gene            complement(2321..2596)
                     /locus_tag="VFF98_12895"
     CDS             complement(2321..2596)
                     /locus_tag="VFF98_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64572.1"
                     /translation="MIKTLLAAAALVALVSPAVADAAPCRTAKGQFAKCGTPGALSDA
                     QYKAQKGKGDGKGAPAAKPAPKPTPAAKPGACKDAKGKFAKCPGQKK"
     gene            complement(2717..4255)
                     /locus_tag="VFF98_12900"
     CDS             complement(2717..4255)
                     /locus_tag="VFF98_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZU64573.1"
                     /translation="MALAALFLALLPAGMAQARPNTAELEQQLRAEIATLASDDFAGR
                     EPGTEGEAKTLRWLAHEWFNIGLVSGTNDPGHPWFAPVELVAREPAGSSALFTRRGRA
                     VPVNQADVLVLTSGRRALIERAPLLFLGHGEVVPARTELAGRVALLLDSRKAGTPAPG
                     GAGSVAAGGLGARQNALLAAGALGVITVLDGERTLAGVIERRRRQGYALASEETGGDL
                     EAFISAAAMDRLLAGSATSLAALQHEADGADFVPRPVDLAASLEATTRQTTIRSHNLI
                     GLLPGRHRQAGAVLVMAHWDHFGTCASPPAEHLICNGAVDNASGVAALTEIARRLAHP
                     PAGTPPLDRDIYFLATTAEEMGLLGAEAFAENPPLPLSQVVAALNLDCVAIAPAGGPL
                     GMVGKGQVSAALEAAIATVAHEQRRSLSASLAPNTYIRRQDGWALMAHDVPAVMVTSA
                     YGDIARLEHYFDTDYHRPQDVMKPGLELGGAAQDVLFHVALVHHLADMRRFDAGAPAN
                     VSAR"
     gene            complement(4502..6310)
                     /gene="sdhA"
                     /locus_tag="VFF98_12905"
     CDS             complement(4502..6310)
                     /gene="sdhA"
                     /locus_tag="VFF98_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZU64574.1"
                     /translation="MTAPAYKIIDHTYDTVVVGAGGSGLRATMGAAEAGLKTACITKV
                     FPTRSHTVAAQGGIAASLCNNTPDHWTWHMYDTVKGSDWLGDQDAIEYMVREAPAAVY
                     ELEHAGVPFSRNADGTIYQRPFGGHMQNMGDGPPVQRTCAAADRTGHAMLHALYQQSL
                     KYDADFFIEYFAIDLIMDNGKCVGVIALCLDDGTLHRFRAQAVVLATGGYGRCYFTAT
                     SAHTCTGDGGGMVLRAGLGLQDMEFVQFHPTGIYGAGVLITEGARGEGGYLTNAQGER
                     FMERYAPSAKDLASRDVVSRSMALEIREGRGVGPHADHIYLHLDHIDPKVLAERLPGI
                     TESGKIFAGVDLTRQPLPVVPTVHYNMGGIPTNYHGEVVTLGPDGNPDQVVQGLFAVG
                     EAACVSVHGANRLGSNSLIDLVVFGRAAGHRLKEIVQQGAKQNELPSDSADLALTRLD
                     HFRNANGGTSTAEIRTEMQRTMSGHAAVFRTDELMAEGKAKLVDTYARMQDIKITDRS
                     LIWNSDLVETLELDNLISQATVTLHGAHARKESRGAHAHEDFPSRDDATWMKHTMAWF
                     EGWGGKGGNVRLDYRPVHEYTLTDDVSYIKPKARVY"
     gene            complement(6310..6693)
                     /gene="sdhD"
                     /locus_tag="VFF98_12910"
     CDS             complement(6310..6693)
                     /gene="sdhD"
                     /locus_tag="VFF98_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZU64575.1"
                     /translation="MGNGTSIGRVRGLGSAHSGAHHWLLQRFTAVGNLVLTIWLAVSL
                     VLLPNYNYITVHDWIVRPIPTVALALLVISVFWHVRLGVQVLLEDYVHEAGSKFAVFA
                     LLNLVVFGAAGFGLFSILRLALGAA"
     gene            complement(6706..7107)
                     /gene="sdhC"
                     /locus_tag="VFF98_12915"
     CDS             complement(6706..7107)
                     /gene="sdhC"
                     /locus_tag="VFF98_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZU64576.1"
                     /translation="MAHNAPGRKARPLSPHLQIWRWGPAMFISIMHRATGNALAVGGI
                     VLLLVWLGALAAGPDVYALFQLIAGSPVGLVVLFGISLVYFWHLVSGIRHFILDIGAG
                     FEVKGNNFWSALVPVFAILLTAAFWAAILLR"
     gene            7345..8337
                     /locus_tag="VFF98_12920"
     CDS             7345..8337
                     /locus_tag="VFF98_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZU64577.1"
                     /translation="MTPADTAAPASETSWKITAHAPRPVIEAALIAHEDAPDWDMDIV
                     LTGFEVAPDRPEDWLLEAYLPRKPRRADLAAIRALFANGAPALTSEELPAEDWITLSQ
                     QELAPIVAGRFCVHTPDFPADAAAPHAFCIPASRAFGTGHHETTAGCLQMLSTMRAEG
                     LQVRNLADIGTGTGLLAFAGLTLWRSAHATASDIDHACVGVVEHNAAVNGVPLGAGAG
                     ALTMVVADGMADPLLEARGPYDLLIANILAGPLIELAPDFARALVPGGSLLLAGLLSV
                     QEDAVRAACRRAGLRIARRLVKGDWSILWLRKRAFAGIRASRPGALPEWARKWG"
     gene            complement(8345..10453)
                     /gene="ligA"
                     /locus_tag="VFF98_12925"
     CDS             complement(8345..10453)
                     /gene="ligA"
                     /locus_tag="VFF98_12925"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZU64578.1"
                     /translation="MTSPDPLSDLPADDAEAANQLMRLARQINRARKLYHLDDAPEIS
                     DPAYDALVRRNEAIEAAFPHLVRADSPTHKVGFEVSASPLAKVAHEVRMMSLDNAFAD
                     EEVVEFVARVRRFLALPAEAPVPLTAEPKIDGLSCALRYEQGRLVRAATRGDGQVGED
                     VTANVAHIADIPQQLVARDLAWPVPDLIEVRGEVYMEKAGFTALNARQEEAGEKLFAN
                     PRNAAAGALRQKDAAITAARPLRFYAHGWGVHSALPAASQSGVMAAIASWGFATALEE
                     WRALDLEAALAYYAALARRRAGLPYEIDGVVYKVDSLEWQQRLGFVAKAPRWAIARKF
                     PAEQAETTLEAIDIQVGRTGKLTPVGRLAPVLVGGVTVTNVTLHNRDEIARLGVRVGD
                     RVVIQRAGDVIPQVVENLSREEARAPFPFPDHCPECGSEAVAEEGEVDVRCTGGLICP
                     AQRTERLKHFVSRGALDIEGLGEKSIEEFFGLGWLESPADIFRLRGRRAEIVGRPGWQ
                     DKSVDNLLMAIEAKRQPDAARLLFGLGIRHVGTVTARDLMKHFASLPELRRVVEAARG
                     ADAAAGEEAAAQLLGIDGVGPVVVEALGEFFHEPHNVAVWEDLLGEVSPPPYVVETVA
                     SEVAGKTVVFTGKLETMSRDEAKAQAERLGAKAAGSVSAKTDLVVAGPGAGTKLKKAA
                     DLGIPVIDEAEWAEIVKRAG"
     gene            complement(10486..12165)
                     /gene="recN"
                     /locus_tag="VFF98_12930"
     CDS             complement(10486..12165)
                     /gene="recN"
                     /locus_tag="VFF98_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZU64579.1"
                     /translation="MLSSLAIRNIVLIEALDLEFSAGLGVLTGETGAGKSILLDALGL
                     VLGMRADSGLIRGGEERASVAAAFEFAALPTALRSLLDEADITIEPGEPLIVKRQLRV
                     DGGSRAFVNDQPVGVGLLREMGGHLVEIHGQHDDRGLVNPRGHRLLLDRFARVDGAAL
                     EAAWKAWAGAEAKLSAARERLAAASAERELLEAHAAELDALAPRPGEEEELAGQRATM
                     QKGEKLAADLAALEHLWSGSDGALAQLRSGARRLDRIAHEHPLLAEALEALDRAVIEA
                     SEAEEKLAAAADALSFDPLALDRIETRLFDLRAAARKHLCAVADLPARAAAMREALEA
                     IEHGAAGIARLEQDAAKAARAYRALAEEASKARAEAAVRLDRAVAAELAPLKLDAARF
                     RTQVQRLGEERWGAGGMDAVEFLISTNPGSDFAPLAKIASGGELSRFILALKVALAEE
                     GGAATIIFDEIDRGVGGAVASAIGDRLARLAGQATQKRGQLLAVTHSPQVAARGGQHY
                     FIAKSSDGTVTRTGVALLDEAGRRDEIARMLSGAEITPEARAQAERLLEGV"
     gene            complement(12242..12481)
                     /locus_tag="VFF98_12935"
     CDS             complement(12242..12481)
                     /locus_tag="VFF98_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64580.1"
                     /translation="MGYGKTGGDGAPGGAGPPAGDAAAGAPAGSEEAEGQARPDPPPA
                     PLRPLAGGRLIYFEHVAIAVIWLLAVGAALFRLLG"
     gene            complement(12588..13079)
                     /locus_tag="VFF98_12940"
     CDS             complement(12588..13079)
                     /locus_tag="VFF98_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU64581.1"
                     /translation="MLSQKTRYTIRALQHLADKFRQGAIRLDAIAEAQNIPRKFLTVI
                     LSEMVREGLVISHRGREGGYELALAPCDIRYGDIIRLTRGSLALVPCASRYAHEHCEN
                     CLPEADCRLRGLMLAVRDEMATMLDRLTLADAIEVEPPFTEAPFAEPPFTKPEDAGLP
                     SQA"
     gene            complement(13213..14022)
                     /locus_tag="VFF98_12945"
     CDS             complement(13213..14022)
                     /locus_tag="VFF98_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZU64582.1"
                     /translation="MSGILSRRSPLKLALVAGVAAATTLVAGCSGHGGKPKDAAYVAR
                     DVESLYLGAKKRLADGDAKTAAALFDEVERQHPYSPWARRAQLMGAFSYYSARDYTKS
                     IESAQRFLSIHPGNKDAAYAYYLIALCYYEQISDVSRDQKDTLQAQSALNDVIRRYPN
                     TRYAEDARLKLDLVIDHLAGKEMDIGRFYERSGRWLAAEIRFRNVIDNYQATSHVPEA
                     LYRLVETNLSLGLPEEAQKNAAVLGHNYPGSEWYDKAYKLMNRAAPGTKAS"
     gene            complement(14184..14804)
                     /locus_tag="VFF98_12950"
     CDS             complement(14184..14804)
                     /locus_tag="VFF98_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64583.1"
                     /translation="MGFTGGMPPVRQPATARRNRNYAETHRLLLERAVAMIGEGGVES
                     LSISELARLSNLNRSTVYYHFESRESLIEEVKGWSAAQLARGFAARGGDYADGIAHIS
                     RFVLGHAEVLRLWIEDFIAPGDIRARYPEWDAMVAGLRRLLGTLSASEEVDPEVVAVM
                     LLAAAFIAPRVYQLSVRPDASAEVVAQRFAEVQRRLLAVRRWSTAG"
     gene            complement(14953..15834)
                     /gene="dapA"
                     /locus_tag="VFF98_12955"
     CDS             complement(14953..15834)
                     /gene="dapA"
                     /locus_tag="VFF98_12955"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZU64584.1"
                     /translation="MFSGSIPALVTPFRDGAFDEPTFRRLVDWQIENGSAALLPCGTT
                     GENSTLSNAEHHRVIEVCIEQAAGRVPVIAGCGSNDTQNAAVHMTFSKKSGAEAALLV
                     APYYNRPSQEGLLAHFSYLAEKIDLPIILYNVPARTVTDIQPETVIELARRFPGRIIG
                     IKDASGDLSRVTDHRMGIGTDFLQLCGDDELALPFNAAGGVGCISVTANVAPRLCADF
                     QAACAAGDLAEARRLNDLLYPLHYAMFEDASPGPLKYALSRVHGWFAEELRLPLVPCN
                     AKARAAVDSALVHAGLA"
BASE COUNT         2589 a         5770 c         5174 g         2465 t
ORIGIN      
        1 ccgggccgcg ccgccaccac gccgcaagcc agctgcgagc cggcatcctg cacctcgatc
       61 aacccctgcg cataggcctc ccactgctcc accggggtgc cggccggcaa gcgcaggccc
      121 tgccgcgtga aagccagcgc ctcgcccgcc accggcagcg ccagcgcggc gcgatccgcc
      181 cttgcggcat taacgcggat atcgagcgcg gcgcggccca gcagcgcctc ggccgcttct
      241 gccgctaggc cgctggccgc cagccgctgc gccagccaga gcggcgccac gccgccatcg
      301 gccgcctgct caccagcggc aagcggcgcg gggccatggg ccgaaccatc gaacagggcc
      361 ctgtcagcca cgcgcagcaa cgccgcccgc ccgctggccg gcaccggccc gcacgcgcgg
      421 atggcggcaa acacatgctc gcggatcgcc cgccgatcgc cactgccggc atagcggcgg
      481 gcgcgcaggc cttctgccac cacccgctcg gccggagcgc cgccggcccg cgccgccgcg
      541 atcacagcgt cgaggatctc gatagcggcc tggaggcgag cggcgggggt catcgggcgg
      601 ggtcctcaag ggggagcgga tggggcaggg gcaagccccc cgccctgccc ctgcccctgc
      661 cagcttaccg cgtcgggtag ttgggcgcct cgcgggtgat cgtcacgtcg tgcacatggc
      721 tttccttcag ccccgcattg gtgatgcgga tgaaattggc cttggtgcgc agatgctcga
      781 tcgtggcgct gccggtatag cccatcgccg ccttgatgcc gcccaccagt tggtgcacca
      841 catccttggc cgggcccttg tagggcacct ggccctcaat gccctcgggc accagcttca
      901 tctggtcctt gatgtcctgc tggaaatagc ggtcggcgct gccgcgggcc atggcgctga
      961 cgctgcccat gccgcgatag ctcttgtagg cgcggccctg gtagaggaaa gtctcgcccg
     1021 gggcttcctc ggtgccggcc agcaggctgc cgatcatcac cgtgctggcg ccggccgcca
     1081 gcgccttggc ggcatcgccg ctggtgcgca ggccgccatc gccgatcacc ggcacgccat
     1141 gctttgtggc ttcctcggcg ctttccatga tcgcggtcag ctgcggcacg ccaacgccgg
     1201 cgacaatgcg cgtagtgcag atcgagcccg ggccgatgcc caccttcacc gcatccgcgc
     1261 ccgcctcgat cagcgcgcgg gtggcctcgg ccgtggcgac attgccggcc accacctgcg
     1321 ccgcattgga aagcttcttc acgcgttcca ccgcgcgggc cacatcgcgg ttgtggccat
     1381 gggcggtgtc gatgatcacc acgtcgcact cggcctcaag cagcgcctcg gtgcgggcaa
     1441 agcccttgtc gcccacggtg gtggcagcgg ccacgcgcag gcggccggcg ccatccttgg
     1501 tggcgttggg ataggtgacg gccttctcga tgtccttcac ggtgatgagg cccacgcagt
     1561 gataggcatc atccaccacc agcagcttct cgatgcggcg ctggtgcagg tgcttgcgcg
     1621 cctcttcctg gctgacgccg gcgcgcacgg tggccaggtt ctcatgcgtc atcagctcgc
     1681 gcacgggctg ggcggcattg tcggcaaagc gcacatcgcg gttggtgaga atgcccacca
     1741 gcttgccatt gccttccacc accgggatgc cggaaatgcg gttggcctgc atgatcgcct
     1801 gcgcctcgcc cagcgtggcg tggggcgaga tggtgatggg gttcaccacc atgccgcttt
     1861 caaagcgctt gaccgcgcgc accgcggcgc actgttcctc gatggtgagg ttgcggtgca
     1921 gcacgccgat gccgcccatc tgcgccatca cgatcgccat gtcggcttcg gtcaccgtat
     1981 ccatcgcggc cgacagcacc gggatgttga gcgcgatctc gcgggtgagg cgcgtgcgcg
     2041 tgtcagccat cgagggcagg atgtcggact ccgccgggcg cagcagcacg tcgtcgaagg
     2101 tcaggccgag ggggatatcg aggattgcca cgctggtctc tcgcttgctg ggaatcgggg
     2161 aggcggattt gtggcggccc atgtaaccaa gcgcggacca aagggccagt gcctattcgc
     2221 acgcggcccg gatgaggtgc acaggccccc aaaaaacgcc aacgcgcggc agggctggcc
     2281 atgccgcgcg ccgggttggg ccggggccct gtcgttcagt tcacttcttc tggccggggc
     2341 atttggcaaa cttgcccttg gcatccttgc aggcgccggg cttggcggca ggggtgggct
     2401 tgggcgcggg cttggcggcg ggcgcgccct tgccatcacc cttgcccttc tgggccttgt
     2461 attgcgcatc gctcagcgcg ccgggggtgc cgcatttggc gaactggccc ttggcggtgc
     2521 ggcagggcgc ggcatcggcc acggccgggc tgacaagggc aacaagcgcg gcggcggcaa
     2581 gcaaggtctt gatcatgggc tctctcctgg gcgagatggc gccggccacg gcgggcgggc
     2641 actgccaaag tgctaggctg gcagccgggc agggtcaatc gccgcgccct gcccgccccg
     2701 ccttaccccg cgtattttac cgcgcgctta catttgccgg cgcgcccgca tcgaagcggc
     2761 gcatatcggc cagatggtgc accagcgcca catggaacag cacatcctgc gccgcgccgc
     2821 ccagttccag cccgggcttc atcacatcct gcgggcggtg gtaatcggtg tcgaaataat
     2881 gctccagccg ggcaatgtcg ccataggccg aggtgaccat caccgccggc acatcatgcg
     2941 ccatcagcgc ccagccatcc tgccggcgga tataggtgtt gggcgcaagg ctggccgaga
     3001 ggctgcggcg ctgctcatgc gccacggtgg caatcgccgc ttccagcgcc gcgctcacct
     3061 gccccttgcc aaccatgccc agcgggccgc ccgccggcgc gatcgccacg caatcgaggt
     3121 tgagcgcggc caccacctgc gagagcggca gcggcgggtt ctcggcaaag gcctcggcgc
     3181 ccagcaggcc catttcctcg gccgtggtgg ccaggaaata gatatcgcga tccagcggcg
     3241 gcgtgcccgc cgggggatgg gccaggcgcc gggcaatctc cgtcagcgcg gccacgccac
     3301 tggcattgtc caccgcgcca ttgcagatga ggtgctcggc cggcgggctg gcgcaagtgc
     3361 cgaaatgatc ccaatgcgcc atcaccagca ccgcgcccgc ctgccggtgg cggcccggca
     3421 acaggccgat gagattgtgg ctgcggatgg tcgtctggcg cgtggtggcc tcgaggctgg
     3481 cggccagatc caccgggcgc ggaacgaaat cggcgccatc ggcctcgtgc tgcagggcag
     3541 cgaggctagt ggcgctgccg gccagcaagc ggtccatcgc cgcggcgctg atgaaggcct
     3601 ccagatcgcc gccggtctcc tcgctggcca gcgcatagcc ctgccggcgg cggcgctcga
     3661 tcacgccggc aagcgtgcgc tcgccatcga gcacggtgat cacccccagc gcgccggccg
     3721 ccagcagcgc gttctgccgc gcccccagcc cgccggccgc cacagaaccc gcgccgcccg
     3781 gcgccggcgt tccggccttg cgcgaatcga gcagcagcgc cacccggccg gccagttccg
     3841 tgcgcgccgg caccacctcg ccatggccca gaaacagcag cggcgcccgc tcgatcagcg
     3901 cccgccggcc agaagtgagc accagcacat cggcctggtt caccggcact gcccgccccc
     3961 gccgggtgaa cagcgccgac gagccggccg gctcacgcgc caccagttcc acaggggcga
     4021 accacggatg gcccggatca ttggtgcccg aaacaaggcc gatgttgaac cattcatgcg
     4081 ccagccagcg cagggtcttg gcctcgccct cggtgcccgg ctcgcggccg gcgaaatcat
     4141 cggaagccaa ggtggcgatc tcggcgcgca gctgctgttc cagctcggcc gtgttgggac
     4201 gggcctgcgc catgccggcg ggcagcaagg ccaggaacag cgccgccagc gccatggcca
     4261 ggcgggggaa aacgcccctc cacccccggc cgcttccccg cagcgccggg ccacccacac
     4321 tatccatcgc gagtctcagg cggctcatcg ccccctgtct acaccaccgg gtgggcgcgg
     4381 caacaatatt gcagaatcaa tccgctcatg cccgcaaacg catctcacgc aggcaaaagg
     4441 ggcgggaagc gccatcgccc ttcccgcccc ttttcatggc accatcagcg cccggccgcg
     4501 atcaatagac gcgggccttg ggcttgatgt agctgacatc gtcggtcagc gtgtattcgt
     4561 gcaccgggcg gtaatccagg cgcacattgc cgcccttgcc accccagccc tcgaaccagg
     4621 ccatggtgtg cttcatccag gtggcatcgt cgcggctcgg gaagtcctcg tgcgcgtggg
     4681 cgccgcggct ttccttgcgg gcgtgggcgc catgcagcgt caccgtcgcc tggctgatga
     4741 ggttgtcgag ctccagcgtt tccaccagat ccgagttcca gatcagcgaa cggtcggtga
     4801 tcttgatgtc ctgcatcctg gcataggtgt cgaccagctt ggccttgccc tcggccatca
     4861 gctcgtcggt gcggaacacc gcggcatggc ccgacatcgt gcgctgcatc tcggtgcgga
     4921 tctccgccgt cgaggtgccg ccattggcgt tgcggaaatg gtcgaggcga gtgagcgcca
     4981 gatcggccga atccgagggc agctcgttct gcttggcgcc ctgctgcacg atctccttca
     5041 ggcggtggcc ggccgcacgg ccgaacacga cgagatcgat cagcgagttc gagcccaggc
     5101 ggttggcgcc atgcaccgag acgcaagccg cctcgccgac ggcgaacagg ccctgcacca
     5161 cctgatccgg attgccatcc ggccccagcg tcaccacttc accgtggtag ttggtgggga
     5221 tgccgcccat gttgtagtgc acggtgggca ccacgggcaa cggctggcgg gtcaggtcaa
     5281 cgccggcaaa gatcttgccg ctttcggtga tgcccggcag gcgctcggcc agcaccttgg
     5341 gatcgatgtg atcgaggtgc aggtagatat ggtcggcatg cgggccaacg ccgcgccctt
     5401 cgcggatttc cagcgccatc gagcgcgaaa ccacgtcgcg gctggccaga tccttggcgc
     5461 tgggggcata gcgctccatg aagcgctcgc cctgggcgtt ggtgagatag ccaccctcgc
     5521 cgcgcgcgcc ctcggtgatc agcacgcccg caccatagat gccggtcggg tggaactgca
     5581 cgaattccat gtcctgcagg ccaaggccgg cgcgcagcac catgccgccg ccatcgccgg
     5641 tgcaggtgtg cgccgaggtg gcggtgaaat agcagcggcc ataaccgccg gtggccagca
     5701 ccacggcctg cgcgcggaag cggtgcagcg tgccatcatc gaggcacagg gcgatcacgc
     5761 ccacgcattt gccattgtcc atgatgaggt cgatggcaaa atattcgatg aagaagtcgg
     5821 cgtcatactt gagcgactgc tgatacagcg cgtgcagcat ggcatggccg gtgcggtcgg
     5881 ccgcggcgca ggtgcgctgc accggcgggc catcgcccat gttctgcatg tggccgccga
     5941 aggggcgctg atagatcgtg ccatcggcgt tgcggctgaa gggcacgccg gcgtgctcca
     6001 gctcgtacac tgccgcaggc gcctcgcgca ccatatattc gatggcgtcc tggtcgccca
     6061 gccagtccga ccccttgacg gtgtcgtaca tgtgccaggt ccagtggtcg ggcgtgttgt
     6121 tgcacaggct ggcggcaatg ccgccctgcg ccgccacggt gtggctgcgg gtggggaaca
     6181 ccttggtgat gcaggccgtc ttcaggccgg cttcggccgc gcccatcgtg gcgcgcaggc
     6241 ccgagccgcc cgcgcccacc accacggtgt cataggtatg gtcgatgatc ttgtaggcag
     6301 gggcggtcat tacgcggctc ccagggcaag gcgaaggatc gagaacaggc cgaagccggc
     6361 agcgccgaac accacgaggt tcagcagggc gaaaacggcg aacttgcttc ccgcctcgtg
     6421 gacatagtct tccagcagca cctgcacgcc caggcgcaca tgccagaaca cgctgatgac
     6481 cagcagcgcc agcgccacag tcgggatcgg gcgcacgatc cagtcgtgca cggtgatgta
     6541 gttgtagttc ggcagcagca ccagcgagac cgccagccag atcgtcagca ccagattgcc
     6601 aacggcggtg aaacgctgca gcagccagtg atgcgcgccc gagtgggccg agccaaggcc
     6661 gcgcacgcgg ccgatggagg ttccgttacc cattgcgtcg tctcctcagc gcagcaggat
     6721 ggccgcccag aaggcagccg tgagcaggat ggcgaagacc ggcaccagcg ccgaccagaa
     6781 attgttgccc ttcacttcga aaccggcgcc gatatcgaga atgaagtggc gaatgcccga
     6841 caccaggtgc cagaaataca caaggctgat gccaaacagc acgaccaggc caacagggct
     6901 gccggcgatc agctggaaca gcgcatagac atcaggcccg gccgccagcg cgcccagcca
     6961 caccagcagc agcacaatgc cgcccaccgc cagcgcattg ccggtggcgc ggtgcatgat
     7021 ggagatgaac atggcgggcc cccagcgcca gatttgcagg tggggcgaaa gcggccgtgc
     7081 ctttcggccg ggggcgttgt gagccatgtc ctcgagtcct cttgcaatgc tgcgatgcgg
     7141 gaaagctccc ttgcgccaat ccccttagcc aaatcgcgcc ggggtgcaag cgcctgtcgc
     7201 cttttgtcgc atgccataaa cgcaaggcag ccctgccaat gcctcatcaa aagaacaagc
     7261 cctgcaaaag gcccgcttgc attttgccgg cacgatagtg cgttctggct tggcgcgggg
     7321 ccctgcgcgg cctatctgcc ggccatgacc cctgccgaca ccgccgcccc cgccagcgaa
     7381 accagctgga agatcaccgc ccacgccccc cgcccggtga tcgaggccgc cctcatcgcc
     7441 catgaagacg cgcccgactg ggacatggac atcgtcctaa ccggcttcga ggtggcgccc
     7501 gaccggccgg aagactggct gctggaagcc tatctgccgc gcaagcctcg ccgggcagac
     7561 ctcgccgcca tccgcgccct gttcgccaat ggcgcgcccg cgctcaccag cgaggaactg
     7621 ccggccgagg actggatcac cctcagccag caggaactgg cgccgatcgt ggccgggcgc
     7681 ttttgcgtgc acacgcccga ttttcccgcc gatgcggccg cgccccatgc cttctgcatt
     7741 ccggccagcc gcgcctttgg caccggccac cacgaaacca ccgccggctg cctgcagatg
     7801 ctgagcacca tgcgcgccga aggcctgcag gtgcgcaacc tggccgatat cggcaccggc
     7861 accggcctgc tggcctttgc cggccttacc ctgtggcgca gcgcccatgc caccgccagc
     7921 gatatcgatc atgcctgcgt gggcgtggtg gaacacaatg ccgcggtgaa cggcgtgccg
     7981 ctgggcgcgg gcgccggcgc gctgacgatg gtggtggccg atggcatggc cgatccgctg
     8041 ctggaagcgc gcgggcctta cgacctgctc atcgccaaca tcctggccgg gccgctgatc
     8101 gagctggcgc ccgatttcgc ccgcgcgctg gtgccgggcg gatcgctgct gctggccggc
     8161 ctgctttccg tgcaggaaga cgccgtgcgc gccgcctgcc gccgggcggg cctgcgcatc
     8221 gcccgccggc tggtgaaggg ggactggtcg atcctgtggc tgcgcaagcg cgcctttgcc
     8281 ggcatccgcg ccagccgccc gggcgccctg ccggaatggg cgcgcaaatg gggctgagcc
     8341 gcgcctagcc cgcccgcttc acaatctcgg cccattccgc ctcgtcgatc accggtatgc
     8401 cgaggtcggc cgccttcttc agcttggtgc cggcgccagg gccggccacc accagatcgg
     8461 tcttggccga aacgctgccg gcagccttgg cgcccagcct ttcggcctgg gccttggcct
     8521 cgtcgcggct catggtttcc agcttgccgg tgaacaccac cgtcttgccg gccacttcgc
     8581 tggctaccgt ttccaccaca tagggcggcg ggctgacctc gcccagcaga tcctcccaca
     8641 ccgccacatt atgcggctcg tggaagaatt cgcccagcgc ctccaccacc accggcccca
     8701 cgccatcaat gcccagaagc tgggcggcgg cttcctcgcc agcggccgca tcggccccgc
     8761 gcgctgcttc caccacccgg cgcagttccg gcaggctggc aaaatgcttc atcagatcgc
     8821 gcgccgtcac cgtgccgaca tggcggatgc ccaggccaaa cagcagccgc gcggcatcgg
     8881 gctggcgctt ggcctcgatc gccatcaaca ggttgtccac agacttgtcc tgccagcccg
     8941 gccggcccac gatctctgcc cgccgcccgc gcaggcggaa gatgtcggcc gggctttcca
     9001 gccagccaag gccgaagaat tcctcgatgc tcttctcgcc cagcccctcg atatcgagcg
     9061 cgccgcggct gacgaaatgc ttcaggcgct ctgtgcgctg ggccgggcag atgaggccgc
     9121 cggtgcagcg cacatccacc tcgccctcct cggccactgc ctcgctgccg cattccgggc
     9181 agtgatcggg gaagggaaaa ggcgcgcgtg cttcctcgcg gctgaggttt tccaccacct
     9241 gcgggatcac atcaccagcg cgctggatca ccacccggtc gcccacgcgc acgcccaggc
     9301 gggcgatctc gtcgcggttg tgcagcgtga cattggtcac cgtcacgccc cccaccagca
     9361 ccggcgccag ccgccccacc ggcgtcagct tgccggtgcg gcccacctgg atgtcgatcg
     9421 cctccagcgt ggtttcggcc tgttcggcgg gaaacttgcg ggcaatcgcc cagcgcggcg
     9481 ccttggcgac gaagcccagc cgctgctgcc attccaggct gtccaccttg tagacaacgc
     9541 cgtcgatctc atagggcagg cccgcccgcc gcctggccag cgccgcataa taggccagcg
     9601 ccgcctccag atcgagcgcg cgccattcct ccagcgcggt ggcaaagccc cagctggcaa
     9661 tcgcggccat cacgcccgac tggctggccg ccggcagcgc cgaatgcacg ccccagccat
     9721 gggcatagaa acgcaagggc cgcgccgcgg tgatcgccgc atccttctgg cgcagggcgc
     9781 cggccgcggc attgcgcgga ttggcaaaca gcttctcgcc ggcctcctcc tgccgggcgt
     9841 tgagcgctgt aaacccggcc ttttccatat agacctcgcc gcgcacctcg atcagatcgg
     9901 gcaccggcca ggccagatcg cgcgctacca gctgctgcgg aatatcggcg atatgggcga
     9961 cattggcggt cacatcctcg cccacctgcc cgtcgccgcg ggtggccgcg cgcaccagcc
    10021 ggccctgctc ataccgcagc gcgcaggaga ggccatcgat cttgggctcg gccgtcagcg
    10081 gcaccggcgc ttctgccggc agcgccagaa accgccgcac ccgggcgacg aattccacca
    10141 cttcctcatc ggcaaaggca ttgtcgaggc tcatcatccg cacctcatgc gccaccttgg
    10201 ccagcggcga ggcggaaacc tcgaagccca ccttgtgcgt gggcgaatca gcgcgcacga
    10261 gatgcgggaa agccgcctcg atcgcctcgt tgcggcgcac cagcgcgtca taggccgggt
    10321 cggagatctc gggcgcatcg tcgaggtggt agagcttgcg ggcgcggttg atctggcggg
    10381 ccaggcgcat cagctggttg gcggcctcgg catcatcggc gggcagatcg gaaagcgggt
    10441 cagggctggt catcgcgcga gccttacggc cattcgcgcc ccgcctcaaa ccccttcgag
    10501 cagtctttca gcctgcgcgc gcgcctctgg cgtgatttcc gcgccggaaa gcatgcgggc
    10561 gatctcgtcg cgccggccgg cctcgtccag cagggcaacg ccggtgcgcg tcacggtgcc
    10621 atcgctgctc ttggcgatga aataatgctg cccgccgcgc gcggccacct gcgggctgtg
    10681 cgtaacggcc agcaattgcc cgcgcttttg cgtggcctgg ccggccagcc gcgccagccg
    10741 gtcgccaatg gcgctggcca ccgcgccgcc cacgcccctg tcgatctcgt cgaagatgat
    10801 ggtggccgcg cccccttcct cggccagcgc cactttcagc gcgaggataa agcgcgacaa
    10861 ttccccgccc gaggcgatct tggcgagcgg cgcgaaatcc gatccgggat tggtggagat
    10921 gagaaattcc accgcatcca tgccgcccgc gccccagcgc tcctccccca gccgctgcac
    10981 ctgggtgcga aagcgcgccg catccagctt cagcggcgcc agctcggccg ccaccgccct
    11041 gtccagccgc accgccgcct cggcccgcgc tttggatgcc tcctcggcca gcgcgcgata
    11101 ggcgcgggcc gccttggccg cgtcctgctc cagccgggca atgcccgcag cgccatgctc
    11161 gatcgcctcc agcgcctcgc gcatggcggc ggccctggcg ggcagatcag ccaccgcgca
    11221 cagatgcttc ctcgccgccg cgcgcaggtc gaacagccgc gtctcgatgc ggtccagcgc
    11281 cagcgggtcg aaagagagcg catcggccgc ggccgccagc ttctcttccg cctcgctcgc
    11341 ctcgatcacc gcccggtcga gcgcttccag cgcctcggcc agcagggggt gttcatgggc
    11401 aatgcgatcc agccgccgcg cgcccgaacg caactgcgcc agcgccccat cagagcccga
    11461 ccagagatgc tccagcgcgg cgagatcagc cgccagcttc tcgcccttct gcatggtggc
    11521 gcgctggccg gccagctcct cttcctcgcc cggccggggc gccagcgcgt ccagctctgc
    11581 cgcatgggct tccagcaatt cgcgctcggc actggcggcg gccagccgtt cccgcgcggc
    11641 agacagcttt gcctccgccc ccgcccaggc cttccacgcc gcttccagcg cggccccatc
    11701 cacccgcgcg aagcgatcga gcagcaggcg atggccgcgc gggttcacca gcccgcgatc
    11761 atcatgctgg ccgtggatct ccaccagatg gccacccatc tcgcgcagca ggcccacgcc
    11821 caccggctgg tcgttcacaa aggcgcgcga tccgccatcc acccgcagct ggcgcttgac
    11881 gatcagcggc tcgcccggct cgatggtgat gtcggcttca tcgagcaggc tgcgcagcgc
    11941 cgtcggcagg gcggcaaatt caaaggcggc ggccacgcta gcccgctcct cgccgccgcg
    12001 aatcagcccg ctgtcggccc gcatgcccag caccagcccc aacgcatcca gcaggataga
    12061 cttgcccgcc cccgtctcgc cggtgagaac gccaaggcca gcgctgaatt ccaggtccag
    12121 cgcctcgatc agaacgatgt tgcgtatggc aagagagctc agcatgttca ccaagtgttc
    12181 tagcacaggt caaaccccgc gtcacctgtc acttgcggta tgtggcgcaa aatttgcgcg
    12241 atcagcccag caggcgaaac agcgcggcgc ccaccgccag cagccagatc accgcgatcg
    12301 ccacatgttc gaagtagatc agccgcccgc cggccagcgg ccgcagcggc gccggcggcg
    12361 gatcgggccg cgcctggcct tctgcctcct cgctgcctgc cggcgcgccg gcggcggcat
    12421 ctccggccgg cgggccggcg ccgcctggcg cgccatcccc acctgtcttg ccatacccca
    12481 tcgcgaaaac ctcgcccctg cctgccacgc gggcggccat catgggcgac agagacaaaa
    12541 gggaaatgag ccggcgcctg aggaaacctc ctggccagcc cacgccgcta ggcctgtgaa
    12601 ggcaggccgg catcttccgg cttggtaaag ggcggctcgg caaacggcgc ctcggtgaag
    12661 ggcggctcca cctcgatggc atcggccagc gtcagccggt cgagcatggt ggccatttca
    12721 tcgcgcaccg ccagcatcag cccgcgcagg cggcaatcgg cctcgggcag gcaattctcg
    12781 caatgttcgt gggcatagcg gctggcacag ggcaccagcg ccaggctgcc gcgcgtcaac
    12841 cggatgatat cgccatagcg gatatcgcaa ggcgccagcg ccagctcata gccgccctcg
    12901 cgcccgcgat gcgagatcac cagcccttca cgcaccattt ccgaaaggat gacggtgagg
    12961 aacttgcgcg ggatattctg cgcctcggcg atggcgtcca gccgaatggc cccctgccgg
    13021 aacttgtcgg caaggtgttg cagggcgcga atcgtatagc gggtcttctg ggacagcatg
    13081 gtggcacagc gcggatcggg aaggcgcaaa agcttgcgcg ggcagcgcct tttatcggcg
    13141 ctctgccccc acaatgtcaa ccacgcagat agaaaacgcg aaggcaggcc tgcccgccct
    13201 cgcgctggca gatcagctgg ccttggtgcc cggcgcggcg cggttcatca gcttgtaagc
    13261 cttgtcatac cactcgctgc ccggatagtt gtggcccagc acagcggcgt tcttctgcgc
    13321 ttcctcgggc aggcccagcg acaggttggt ttccaccagg cgatacagcg cctcgggcac
    13381 gtggctggtg gcctggtaat tgtcgatcac gttgcggaag cggatttccg ccgccagcca
    13441 gcggcccgaa cgctcgtaga agcggccgat atccatttcc ttgccagcca gatggtcgat
    13501 caccagatcc agcttcagcc gggcatcctc ggcatagcgg gtgttgggat agcggcggat
    13561 cacatcgttc agcgcgcttt gcgcctgcag cgtgtccttc tggtcgcggc tcacatcgct
    13621 gatctgctcg taatagcaca gggcgatgag gtaataggcg taggccgcat ccttgttgcc
    13681 ggggtggatc gacaggaagc gctgcgccga ttcgatcgac ttggtgtaat cacgcgccga
    13741 ataataggag aaggcgccca tcagctgcgc ccggcgggcc cagggcgaat agggatgctg
    13801 gcgctccacc tcgtcgaaca gggccgcggc ggtcttcgca tcgccatcgg ccagccgctt
    13861 cttggcgccc agatacagcg attccacatc gcgcgccaca taggcggcat ccttgggctt
    13921 gccgccatgg cccgaacagc ccgcgacaag cgtggtggca gcggcaacac cggcgacaag
    13981 cgccagcttg aggggcgaac ggcgagagag gatcccagac atgtctcggc ctatagccgg
    14041 cccgcagccg aacgcaaagg cccaacgcaa caggccgcaa cgccatccgc caccggcctg
    14101 cccgcccccg cagggcagcc ggcaacagcg ccgcaatgcc gccgcaacaa tcgcgcaaca
    14161 cactggtgca tcggcataat ccgtcagccc gccgtgctcc agcggcgcac cgccagcagc
    14221 cggcgctgca cctcggcaaa gcgctgggcc accacctcgg cgctggcatc gggccgcacc
    14281 gacagctggt agacgcgcgg ggcgatgaag gcggccgcca gcagcatcac cgccaccact
    14341 tcaggatcca cttcctcgct ggccgaaagc gtgcccagca gccggcgcag ccccgccacc
    14401 atcgcatccc attccggata gcgcgcgcgg atatcacccg gcgcgatgaa atcctcgatc
    14461 cacagccgca gcacctcggc atggcccagc acgaagcggc tgatatgggc gatgccatcg
    14521 gcataatcgc cgccccgcgc cgcaaaaccg cgcgccagct gcgcggcaga ccagcccttc
    14581 acttcctcga tgaggctctc gcggctttcg aaatgataat agaccgtcga acgattgaga
    14641 ttgctgaggc gggccagttc ggagatcgac aggctttcca ccccgccctc cccgatcatc
    14701 gccacagctc tctccagcaa gagcctatgg gtttccgcat agttgcgatt gcgacgggcg
    14761 gtagcgggct gtcggaccgg aggcatgcct ccggtgaaac ccattttttg ttgctaaatc
    14821 aagatgattt aacggcttcg ttcatcttgc gacaaaccgg cattgttgtt tgaatcatat
    14881 ttcgcacagc acagccaact tcgccgcaag attgccccgc cggcccggtt atcaccccgg
    14941 gccggccggc gctcaggcca ggcccgcgtg caccagcgcc gaatccaccg cagcgcgcgc
    15001 cttggcgttg cagggcacca gcggcaggcg cagttcctcg gcgaaccagc catgcacccg
    15061 gctcagcgca tacttgagcg ggccgggcga ggcatcctcg aacatggcat aatgcagcgg
    15121 atagagcagg tcattcagcc ggcgcgcctc ggccagatcg ccggccgcgc aggccgcctg
    15181 gaaatcggca cacaggcgcg gcgccacatt cgccgtcacc gagatgcagc ccacgccgcc
    15241 ggccgcattg aacggcagcg ccaactcgtc atcgccgcac agctgcagga aatcggtgcc
    15301 gatgcccatg cgatggtcgg tcacgcgcga gagatcgccg ctggcatcct tgatgccgat
    15361 gatccgcccc ggaaaacgcc gcgccagctc gatcaccgtt tccggctgga tatccgtcac
    15421 cgtgcgggcg ggcacgttgt agaggatgat cggcaggtcg atcttctcgg ccagatagga
    15481 gaaatgggcc agcagccctt cctggctggg gcggttgtaa tagggcgcca ccagcagcgc
    15541 cgcctcggcg ccagacttct tcgagaaggt catgtgcacg gcggcattct gcgtgtcgtt
    15601 gctgccgcag ccggcgatca ccggcacccg gccggcggcc tgctcgatgc acacctcgat
    15661 cacccggtgg tgctcggcat tcgagagcgt ggaattctcg ccggtggtgc cgcagggcag
    15721 cagcgcggcc gagccgttct cgatctgcca gtcgaccagg cggcggaaag tgggctcatc
    15781 gaacgcccca tcgcgaaaag gcgtcaccag ggccggaatg gaacccgaaa acatggactt
    15841 tattccttgc gttgccccac cctatagggg tggcgagcga cgccccagac gcgccgccat
    15901 taatgcctgc gcctgataag gagccttgcc ccccgatgtc cagtatcatg atgcctgccg
    15961 ctggcccgca gatgcgccct cgcggggcgt ggcggcgg
//
LOCUS       DASRYE010000255        12293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_227330,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000255 DASRYE010000000
VERSION     DASRYE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227330"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFF98_12960"
     CDS             complement(<1..858)
                     /locus_tag="VFF98_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZU64585.1"
                     /translation="MKTFSTPARPPARAPARPWADIGSFALIGLSWGSTWLVIKDQVS
                     LAPVGWTVTFRFALAAAGMFALALLRRERLWLGRRGLALASLAGVLQFCANYQFVYRG
                     ERFVTSGLVAVIYALLMVPNALLGRLVTGARASGRFWLGSGVAMAGIGLLLLNEVQAA
                     RSAGAAALGIGFALLGMLCASVSNVAQSLPEVRRHGLLAFTGWAMLAGMLADAAFAIA
                     TDGAPTWPVQWRYWGGVGWLAVVGSVLTFPVYYRLILRLGAGRAAYVNVVTPVVAMAL
                     STLVEGYRWG"
     gene            1020..1556
                     /locus_tag="VFF98_12965"
     CDS             1020..1556
                     /locus_tag="VFF98_12965"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZU64586.1"
                     /translation="MTAEELQALVRTVPDFPKPGILFRDITTLIGHGAGFAASVALLA
                     DKARAAGAEAIAGMEARGFIFGAAVAARLGLPFVPVRKPGKLPVPVLAIDYALEYGTD
                     TLEVDPGAIAQGQKVVILDDLIATGGTALAAVQLLRRAGAQVDHALFVIDLPELGGAD
                     RLRAQAITVDPLMAFDGH"
     gene            1686..1759
                     /locus_tag="VFF98_12970"
     tRNA            1686..1759
                     /locus_tag="VFF98_12970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1718..1720,aa:Gly,seq:tcc)
     gene            1957..3189
                     /locus_tag="VFF98_12975"
     CDS             1957..3189
                     /locus_tag="VFF98_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZU64587.1"
                     /translation="MALTDVAIRNAKPKDKPYKLGDALGLFLLVQPTGGKLWRMKYRV
                     DSKEKKLGLGTYPEISLAEARKRRDDAREMVAMGKDPSREKQRDKVRAKLEADNTFGA
                     ITDEYCKKRRRDGAKAWAPATAVRSEYLLSLLKPALGAMPIGEIEPADVLGAIRKIEG
                     KGNLESARRTLQLASAVFRYAVATARLRSDPTRDLKGALTAPTVKHYAAIIEAPKVGD
                     LLRAIDGYEGNGLTKLALQIAPHVFVRPGELRHAEWSEFDLDGALWTIPAEKMKMRKP
                     HHVPLSRQAVALFKEVQAVTGPAGYVFPSLRTRARPMSENTLNAGLRRLGYSSDEMTA
                     HGFRAMASTLLNESSKWNADAIERALAHGADGKVRGAYHRGPHWEERVKMGQWWSDYL
                     DQLRKGAEVVAFPSQGAA"
     gene            3284..4081
                     /locus_tag="VFF98_12980"
     CDS             3284..4081
                     /locus_tag="VFF98_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZU64588.1"
                     /translation="MRLMAVVSSGLALCVGQTAHANDWEKFYRPIDSFSSSVIDYDGE
                     PEMLNSSGNLDGDIEQMWRKGFAPIGYTSFETGNNKTGDAVRFAKKLKARYLIVHANL
                     QSSRTTSMPLTLPNTTTSYTNGNASAYGSGGYAHGTYSGTTTTYGTKTTYIPITVNRF
                     SKAAIYFQEVPKIGFGVFSRELTPAEMQRLETRHAVAVRFVRDGSPAYVADIFPGDII
                     TKINGLPVGTEVVKAALIGPQPVTLHVDRNGQQKDISVNVPPEWQPK"
     gene            4238..4930
                     /locus_tag="VFF98_12985"
     CDS             4238..4930
                     /locus_tag="VFF98_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64589.1"
                     /translation="MSNDSEEWIPLRKAVAEVAAAYSQFSPCAPVVATENSLKAIMRS
                     IDRWNCAAQASYWSVVFYNEKKTRQVDSNKLGNRLPPSIFRGLESATKIWICDWVIGE
                     YCYEWKREDSSYTQFHVAKDIDVDRRNLPLIGQSKGVDNFDLNHFERALREEKRGARR
                     KWDWEGAICEILAYANYHPDGLPDGYGAQAEIGRMLANWFRIHQDGEPAQSEIGTRAA
                     KIMQAVESHRKI"
     gene            5376..5582
                     /locus_tag="VFF98_12990"
     CDS             5376..5582
                     /locus_tag="VFF98_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64590.1"
                     /translation="MPQSMRVPAHACHPEPLAYSIADACRVSSIGKTRLYQLINEGKL
                     EARKLGKRTLIPAASLRRLIAGEG"
     gene            5586..5780
                     /locus_tag="VFF98_12995"
     CDS             5586..5780
                     /locus_tag="VFF98_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64591.1"
                     /translation="MRQNNKTPAGIADQAGASRDGWRAASLFTFSLDAYRAQFLMCAY
                     AVRPEIAVMLAVWALRGANG"
     gene            5773..6369
                     /locus_tag="VFF98_13000"
     CDS             5773..6369
                     /locus_tag="VFF98_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64592.1"
                     /translation="MADWLDAEMRAAARLVRQPHLDRLRALGCTGTALARLGEHHAPY
                     GTMRIAARTGGFYEPCAAGQPALIVPVCWPEHHEGLITPLITLWPIIDLIAVRADYPA
                     NWLWRTGTAWALGGHLLEDWQGEPVPVVATPLDWLRAAGGAVCVLDWADNSPAWPILR
                     RLPHIVASDDLLARRLAQAIDRTAPRPLISVGGRRHAA"
     gene            6359..7879
                     /locus_tag="VFF98_13005"
     CDS             6359..7879
                     /locus_tag="VFF98_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HZU64593.1"
                     /translation="MPLNLAAMGADASPDWPEPLPLNDELPAVLPFDPAMLPSQLAPW
                     VIDIAERMNCPLDMVAVPAMIAAGSLVGRRIAIRPQRQTDWQEAGNLWGAVVAPPGHM
                     KSPAASEALKPLRRLESQAALANARAQEQFRLAEALHKVQSRDAETEAKKRLKEGSHD
                     AAMAALRAAQPPELPPERRYLANDATAEKLGEMCAANPFGLMVHRDELLTLFADLDQS
                     EKATARGFFMVGWGGQEGYTFDRIGRGTVRVSTVNISLFGTTQPTRLAGYVRESLRRF
                     DDGLVQRLQLLAWPDFSGEYREVDRYPNSTARQQAYACFDDLAALNVNEIGAERDTYA
                     GDDGVPFLRFAPAAQEAFCTWRAELERKARGDDLAPALSSHLAKYRGLIPRLALVCHL
                     AGNGLGPVSMAAANQSFAWAAYLESHARRVYASLSLDNAEAARAIWRRIVRRDLADGF
                     TVRDIQRKQWAGLGDKARIEAGLEALLQVDWLRREAVDTGGRPSVVYHVNPKALRR"
     gene            7968..8672
                     /locus_tag="VFF98_13010"
     CDS             7968..8672
                     /locus_tag="VFF98_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64594.1"
                     /translation="MPKANPKPQPTKPKGPNNSLRIAPGENDSNALATARAIMAPSVR
                     HGMANAQLLQKHFDGSPSAPGFGDYAQALRERGDKAADGDLAFVSRMLAAQASTLDTI
                     FAETARRMAYNMGEYMGAAETYARIAMKAQAQCRATLEALTKMHSSKEQTVRHVHVNE
                     GGQAVIADEFHHHASQQTGEQNFGQADKQPHAPGKAGGAAGAGAALPGPNACGAGVPI
                     PGDQGRETVPDARRQG"
     gene            complement(8995..9360)
                     /locus_tag="VFF98_13015"
     CDS             complement(8995..9360)
                     /locus_tag="VFF98_13015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZU64595.1"
                     /translation="MTTAINSVTIAGHIDFDPADCPAVLLEARAHIEGARGQPGCVAY
                     TWAFDPLVPGRVQVFEEWDSEQALHDHFAGPHYRDMGGHLAAVGMRGFSIKMYGVAVL
                     EPVYDENNRPRPLFFAGKR"
     gene            complement(9458..11569)
                     /locus_tag="VFF98_13020"
     CDS             complement(9458..11569)
                     /locus_tag="VFF98_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HZU64596.1"
                     /translation="MIGRRGLLRGAMGGAGLAALPGGLGAVLARAAAIGPDRRRGTLG
                     DVGHIVVLMQENRSFDHYFGTMPGVRGFADRFPIPVPAAADGAPLQVWDQRDGAADPP
                     RRMRPFPLVTSADFDLMRMQGTPHSWANAQRAWDNGRMAHWPEAKTQRAMAYYTRADI
                     PFQWAMAEAFTLCDAYHCALHAGTNTNRLFLWTGTNDPCGRGGGPAVDNPEDDLNRPD
                     AASTPRFSWTTYPERLEAAGVSWAVYQDMADNFGDNPLANFLQYRAAWRGDAGARRVL
                     AQKGLATRALDALRADVLADRLPQISWIVGTAEGSEHPGPSSPAQGAAYTAQVIAALT
                     ANPAVWARTVLFVNFDENDGFFDHVPPPAPPSPDADLPGGFAGASTASTQGEYHRLPA
                     PGAEDDDPELRQRPYGLGPRVPMYVLSPWSRGGRIHSEVADHTSVIRFMEARFGVREP
                     HISPWRRAVCSDLTTAFDFAGAARVPQGLPPALAESLAAAPRRAARARAIARQPDPAP
                     PLAQPLPAQLPGVKPACSARYRLDVRGALAAGGRALRLAFAAGAGLAGVFHVYDRLRL
                     DLVPRRFTVEPGRQIEDEWPLHGEARAYDLWVLGPWGFHRHFVGGAAAEPELRWALLE
                     DRLRLSVAPGHGGLVVAPQAYAAMHGRWAPGPEGGTMDWPLAASCGWYDLLATGPGGW
                     QRRLAGCLAGPWPAITDPARG"
     gene            complement(11566..>12293)
                     /locus_tag="VFF98_13025"
     CDS             complement(11566..>12293)
                     /locus_tag="VFF98_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HZU64597.1"
                     /translation="RHYAPALRRMAQVLGCDRARPPCRLAALPGAIAPAADGRSLAFS
                     GPVEQASGTAQIFLLQYMEGFAPAEVGWGRAGPAVIAALSPLHAALFDVFDRSPYMAP
                     RSASVLARRMLSALFDAGQPRLTLLVGHDNNIAAMGALLRTRFRVPGLGWNDPPPGGA
                     LGLALLRRADGRQMVRAFYVAARPGQVRRLQALEGGHGPAEMALPIGLCGRQALACPA
                     PLLRRALAARLLPLAPIAAGAQP"
BASE COUNT         2352 a         3990 c         3927 g         2024 t
ORIGIN      
        1 cccccagcga tagccttcca ccagcgtgga caaggccatc gccaccaccg gcgtgacgac
       61 attgacatag gccgcccgcc ccgcccccag ccgcaggatc agccggtagt aaaccgggaa
      121 ggtgagcacc gagccaacca ccgccagcca gcccaccccg ccccaatagc gccactgcac
      181 cggccaggta ggcgcgccat cggtggcgat ggcaaaggcg gcatcggcca gcatgcccgc
      241 cagcatcgcc cagccggtga aggccagcag gccatggcgg cgcacttccg gcaggctttg
      301 cgccacattg gagacagacg cgcacagcat gcccaaaagc gcaaagccaa tgcccagcgc
      361 cgccgccccg gctgatcgcg cagcctgcac ctcgttcagc agcagcaggc caatgcccgc
      421 catcgccacc ccagagccca gccagaaccg cccgctggcc cgggcccctg tcaccagccg
      481 ccccagcagc gcattgggca ccatcagcag cgcatagatc accgccacca gccccgacgt
      541 gacgaaacgc tcgccgcgat agacgaactg gtaattggcg cagaactgca gcacgccggc
      601 caggctggcc agcgccagcc cccgccggcc cagccacagc cgctcgcgcc gcagcagcgc
      661 cagggcgaac atgccggccg ccgccagggc aaagcggaag gtcaccgtcc agcccaccgg
      721 cgccagcgac acctgatcct tgatgaccag ccaggtggac ccccagctga ggccgataag
      781 cgcaaaggag ccgatatcgg cccagggacg ggcaggcgca cgggcaggtg ggcgggcagg
      841 cgtggaaaag gttttcatca ccccctgccg gtgcgggagc cggcgcgctg cgtcaagccg
      901 gcaaatcggc aggcaggcgg cggcggcctg ccggcgcgca cgcaaaagcg ctttgccttc
      961 cgccccccaa gcggctagtc ggcgcgcatc cccccaaggg tttcacgcag gaaagcatga
     1021 tgacggccga agagttgcaa gcgctggtgc gcaccgttcc cgattttccc aagccgggca
     1081 tcctgttccg cgatatcacc acgctgatcg gccatggcgc cggctttgcc gcctcggtgg
     1141 cgctgctggc cgacaaggcc cgcgccgccg gcgccgaggc gatcgccggc atggaagcgc
     1201 gcggcttcat ctttggcgcg gcagtggcgg cgcggctggg cctgcccttc gtgccggtgc
     1261 gcaagcccgg caagctgccg gtgccggtgc tggccatcga ttatgcgctg gaatatggca
     1321 ccgacacgct ggaagtggac ccgggcgcca ttgcccaggg ccagaaagtg gtgatcctcg
     1381 acgatctcat cgccactggc ggcaccgcgc tggccgcggt gcaactgctg cgccgcgccg
     1441 gcgcgcaggt ggaccatgcg ctgttcgtga tcgacctgcc cgaactgggc ggcgccgaca
     1501 ggctgcgcgc ccaggccatc accgtggatc cgctgatggc cttcgacggc cactgagcac
     1561 aaggtacggg gaacggggta cggaaccggc gcgcccggcg caaagaagcg attgacgccg
     1621 gcgccccgcc cgtggcaagc gcatcgccac accggagcct tggggccaag ccccttcgcc
     1681 cccgcgcggg tatagcttag tggtaaagct ccagccttcc aagctggcta tgcgggttcg
     1741 attcccgcta cccgctccac tttccagacc gctaccgttc gccactgtct ggaaaagcct
     1801 cggaaatccg ctatattgcg cccatatctg gccatcagcg ttcgcccccg ttcacagatt
     1861 gccacccgct tttgggggcc actgtggggg ccatttgctg gaggccgccc gatggggcct
     1921 caatctgggg gccacttggc ctgaaagtgg cggaaaatgg cgcttaccga cgttgcaatc
     1981 cgaaacgcga aacccaagga caagccctac aagttgggcg atgcgctggg cctgttcctg
     2041 ctggtgcagc ccaccggggg caagctgtgg cggatgaaat accgtgttga cagcaaggag
     2101 aaaaagctgg ggcttggcac ctatccagaa atcagccttg ccgaggcccg caagcgccgc
     2161 gatgatgccc gcgaaatggt ggccatgggc aaagaccctt cccgcgaaaa gcagcgcgac
     2221 aaggtgcggg cgaagctgga ggcggacaac acttttggcg cgatcaccga cgaatattgc
     2281 aagaaacgca ggcgcgatgg ggccaaggca tgggcgcccg caacggcggt gcgcagcgaa
     2341 tatctgctgt ccctgctgaa acccgctttg ggggccatgc cgattggcga gattgaaccg
     2401 gcggacgttc tgggggccat tcgcaagatc gagggcaagg gcaatctgga aagcgcccgc
     2461 cgcacgctgc aactggccag cgcggtgttt cgctatgccg tggcgaccgc acggctgcga
     2521 tctgacccca cccgcgacct gaaaggcgcc ctgacagccc ccaccgtcaa acactatgcc
     2581 gcgatcatcg aggcccccaa ggtaggcgac ctgctgcgcg ccattgacgg ttacgagggc
     2641 aacgggctga cgaagctggc gctacaaatc gcgccccatg ttttcgtgcg ccccggcgaa
     2701 ttgcgccatg ccgagtggag cgagtttgat ctggatgggg cgctgtggac catcccggcg
     2761 gaaaagatga agatgcgcaa accccatcat gtgccccttt cccgccaagc cgtggctttg
     2821 ttcaaagagg tgcaggcggt gacaggaccg gcgggctatg ttttcccctc gcttcgcacg
     2881 cgcgcgcgac ccatgagcga aaacacactg aacgctggct tgcggcggct gggctattcc
     2941 agcgatgaaa tgaccgccca tggctttcgg gcgatggctt caaccttgct caacgaatcg
     3001 agtaagtgga atgccgatgc catcgaacgg gcactggcgc atggtgcaga tggcaaagtg
     3061 cgcggtgctt atcatcgggg gccgcattgg gaggagcggg tgaagatggg ccaatggtgg
     3121 agcgattacc tcgatcagct acgcaaaggc gccgaggtgg tggccttccc aagtcaaggg
     3181 gccgcataat tcgaggatgg ctcaggattg tgaggattat tattttcatt cagacttata
     3241 atccagtcga attatgattc ttgcatttct ggagggggct tgaatgcgct tgatggccgt
     3301 tgtttccagt gggctggccc tttgtgtcgg ccagaccgct cacgctaacg attgggaaaa
     3361 attttatcgc cccattgaca gcttttcgtc gtcggtgatc gactatgatg gcgaacctga
     3421 aatgctaaat tccagtggca atttggatgg cgatattgag caaatgtggc gcaaagggtt
     3481 tgcccctatt ggctatacgt cctttgagac tggcaataac aagacaggcg atgcagtccg
     3541 attcgcgaaa aaactaaagg caagatacct aattgttcat gccaatcttc agtcttcgcg
     3601 cacaacttct atgcccttaa ctctacccaa caccacaacc tcatatacca atgggaatgc
     3661 atcggcctat ggtagcggag ggtacgctca cggtacatat tcaggaacga cgaccaccta
     3721 tggcactaaa acgacctata ttcccataac agtcaaccgc ttcagcaagg cagccatata
     3781 ttttcaagag gtgccgaaaa tcggttttgg tgtctttagc cgagaattga cacctgcgga
     3841 aatgcagagg cttgaaacgc gacacgcagt tgccgtccgc tttgtacgcg atggctcacc
     3901 ggcctatgtg gccgatatat tcccgggcga tatcatcacc aaaattaatg ggctccctgt
     3961 ggggaccgag gtagtgaaag ctgcgctcat aggcccgcag cctgtaaccc tccatgttga
     4021 tcgcaacgga caacagaaag acatttcagt gaacgtgcca cccgagtggc aaccgaaatg
     4081 actgcttacc gatctgtgca aagcccttgt tgggcgactg acaaaactga caaaagatgc
     4141 atgatccacg cctgtcggca ggatagccag ccgacaggca caacactccg acgcgcctcc
     4201 ccgcttgccc ttcggagccg ctgccgaggg cggattgatg agcaacgatt cagaagaatg
     4261 gatcccactc cgaaaagctg tcgccgaggt ggcggcggca tactcgcaat tttcgccatg
     4321 cgccccagtg gtcgcaacgg aaaattccct gaaggccata atgcgctcaa tcgaccgatg
     4381 gaattgtgcc gcccaagctt cttactggtc agttgtcttc tataacgaga aaaaaacacg
     4441 gcaggtcgat agtaataaac taggaaatag acttccacca tccatttttc gagggctgga
     4501 aagtgctaca aaaatatgga tctgtgattg ggttatcgga gaatattgtt acgagtggaa
     4561 acgcgaggat tcatcctaca cccaattcca tgttgcgaaa gacattgatg ttgatcgccg
     4621 gaatttgcca cttatcggcc aaagtaaggg tgtagacaat tttgacttaa atcattttga
     4681 gcgagcactg cgtgaagaaa agcgtggagc aaggcgtaag tgggattggg agggagcaat
     4741 ttgcgaaatc cttgcttatg ccaactacca cccggacggt ttgccagatg gttatggcgc
     4801 acaggcagag attggtcgga tgctagcgaa ttggtttcgt attcatcagg atggggagcc
     4861 cgcccaaagc gagattggca ctcgcgcagc aaagattatg caagctgtcg agagccaccg
     4921 gaaaatataa ttccggtttc atccggtaat tccggctgac caatcgccaa tgaacgggga
     4981 taatcgatgc catcattcac tgacgaaagt ttaagcatga gtaccatcgc tgtggcgcgg
     5041 cacgaaacaa atgggtggaa atggccggcc ttcatgctgg cctgcaaccc cgacgcggac
     5101 taagatggtt gtcattgagt tcatacgcaa acccgaggac cgcatctcgg ttttgcttat
     5161 ggccttaata cctgcccctt caacccaaac caacagacac ggaatatcca atgccctaga
     5221 gaacgaaacc ccgcccggta gggggcgagg ctccgagcga taagattggc gtcccttcgc
     5281 tctgagcttc ctactctcgc cggataattc cgacaatccc ctaaagcgaa cgcaggcgaa
     5341 cgctacttgc ggcctgccca gtaggaagcc ttcacatgcc tcagtccatg agagtgcctg
     5401 cgcacgcctg tcatcccgaa cctctcgcct attccattgc cgacgcctgc cgcgtctcga
     5461 gcatcggcaa gacccgcctt taccagctta tcaacgaggg caagcttgaa gcccgcaagc
     5521 tgggcaagcg cactttaatc ccggctgcca gcctgcgccg cctcatcgct ggggagggct
     5581 aagccatgcg ccagaacaat aaaaccccgg ctggcattgc agaccaagcc ggggcttcac
     5641 gcgatggctg gcgggccgcg tcgctcttta cctttagcct tgatgcttac cgggcgcaat
     5701 ttctgatgtg cgcctatgcg gtgcgccccg agattgccgt tatgttggcc gtgtgggccc
     5761 ttcggggtgc caatggctga ctggcttgac gctgaaatgc gcgccgccgc gcgccttgtg
     5821 cgccagccgc accttgatcg cctgcgcgcc ttgggctgca ctggtacggc cctggcgcgg
     5881 ctgggtgagc atcatgcacc ctatggcacc atgcgcatcg ccgcgcgcac gggcgggttt
     5941 tacgagccgt gtgcggctgg ccaaccggcg ctgattgtgc cggtatgctg gcccgaacat
     6001 cacgagggct tgataacgcc gctcatcacc ctgtggccga tcatcgatct gattgcggtg
     6061 cgcgcggatt atccggccaa ttggctttgg cgcaccggca ctgcctgggc gctgggcggg
     6121 cacttgctgg aggattggca gggcgaaccg gtgccggtgg tggccacccc gctagactgg
     6181 ctgcgcgctg ccggtggagc ggtgtgcgtg ctggactggg ccgacaatag ccccgcctgg
     6241 ccgatactgc gccggttgcc gcacattgtt gccagcgatg atttgctggc gcgccgcctt
     6301 gcacaagcta ttgaccgcac cgcgccccgg ccccttatca gcgtgggggg ccgccgccat
     6361 gccgcttaat ctggctgcca tgggggccga tgccagcccc gactggcccg aacccttgcc
     6421 gctgaatgat gaattgccgg cggtgctgcc gtttgatccc gcgatgttgc ccagccaatt
     6481 ggcgccttgg gttatcgaca ttgccgagcg catgaactgc ccgctggaca tggtggcggt
     6541 gccggcgatg attgcggctg gctcgctggt ggggcggcgc attgccatcc gaccccagcg
     6601 ccaaaccgat tggcaggagg cgggcaatct gtggggcgcg gtggtagcgc cacccggcca
     6661 tatgaaaagc ccggcagcta gcgaggcgtt aaagccgttg cgccgtctgg agtcccaagc
     6721 ggcgcttgcc aatgcgcggg cgcaagagca attccgactt gccgaggccc tgcacaaggt
     6781 ccaatcgcgt gatgccgaaa ccgaggccaa aaaacgcctg aaagagggca gtcacgatgc
     6841 ggccatggcg gcgctaaggg ctgcccagcc gccagaattg ccgcccgaac ggcgctacct
     6901 cgccaacgat gcaaccgctg agaagctggg cgaaatgtgc gcggccaatc cctttgggct
     6961 aatggtgcat cgcgatgaat tgctgacctt gtttgccgat ctagatcaaa gcgaaaaggc
     7021 caccgcgcgc ggctttttca tggtgggctg gggcgggcag gagggatata cctttgatcg
     7081 catcgggcgg ggtacggtgc gggtgtcgac ggtcaatatc tcgctattcg gcaccacaca
     7141 gcccacacgg cttgccggct atgtgcgcga aagtctgcgc cgtttcgatg atgggctagt
     7201 gcagcgcctg caattgctgg cctggccgga tttcagcggc gaatatcgcg aggttgaccg
     7261 ctaccccaat agcacagccc gccagcaagc ctatgcctgt tttgatgatc tggccgcgct
     7321 gaatgtgaac gaaatcggcg ccgagcgtga tacctatgct ggcgatgacg gtgtaccatt
     7381 cctgcgtttc gcgccagccg cgcaggaggc gttttgcaca tggcgcgccg aacttgaacg
     7441 caaagcgcgc ggcgatgacc ttgcgccggc cttgtcctcc catctggcga aatatcgcgg
     7501 cctaatccct cggctggcgt tggtttgcca tttggcgggc aatggcctgg ggccggtgtc
     7561 gatggcggcg gccaaccagt cattcgcctg ggcagcctat ctcgaaagcc acgcacggcg
     7621 ggtttacgcc agcctctcgc tcgacaatgc cgaggccgcc cgcgcgatct ggcggcggat
     7681 tgtccggcgc gatctagccg atggcttcac cgtccgcgac attcagcgca agcaatgggc
     7741 gggcctgggc gacaaggcgc gcatcgaggc cgggcttgag gcgttgctcc aagtcgattg
     7801 gttgcggcgc gaggcggttg ataccggggg gcggccttcg gtggtctatc acgtcaaccc
     7861 caaggcctta cggcgctaac ccagccgcac ccctcggtgg gggggctttt gtcagttttg
     7921 tcagccccct gcccgccatt gcccgatatt tgagaaggaa cttctccatg cccaaggcaa
     7981 accccaaacc ccagcctacc aaacccaagg ggccgaacaa tagtttgcgt attgcccccg
     8041 gtgaaaatga cagcaacgcg ctagccaccg cgcgcgccat aatggcgccc agcgtccggc
     8101 acggcatggc caatgcccag cttttgcaaa agcatttcga tggctcgcca tcggcgccag
     8161 gctttggcga ttatgcccag gccttacgtg aacgtggcga caaggccgcc gatggcgacc
     8221 tcgcctttgt tagccggatg ctggcggccc aggcctccac gctcgatacc atctttgctg
     8281 aaaccgcccg gcgcatggct tacaacatgg gcgaatatat gggggccgct gaaacctatg
     8341 cacgcatcgc catgaaggcc caggcgcaat gccgggccac gctggaagcc ttgaccaaga
     8401 tgcacagttc caaagaacag accgtacgcc acgttcacgt aaacgagggc gggcaagctg
     8461 tgattgccga tgaatttcac caccacgcca gccaacagac aggggagcag aattttggac
     8521 aagccgacaa acaaccccat gcacccggaa aggctggagg cgcggctggc gcgggcgccg
     8581 cgctgccagg cccgaacgcg tgcggggcag gcgtgccaat ccccggcgat caagggcgcg
     8641 aaacggtgcc ggatgcacgg cggcaagggt agtggcgcgc ccgctggcaa tcacaatgcc
     8701 atgaagcatg gggcaagatc tggcgaaaca atgcgcatga aggccgtgat ccgggcgatg
     8761 ggcgacgcgc tggaatggga tggaggggta gggtaatctg tggggcggcg ctggcaaagg
     8821 cacgccattt gaccgccccc gttcgctgcc attcgcagta tttgggggcc acattggggg
     8881 ccactttcaa atcaaattca atgttatgaa tatatttcag ataatgagat aattttcgcg
     8941 gttcccgcta cctccatttt cccatccgcc cacaaagggg tccctggccg gccctcagcg
     9001 tttgccggcg aagaacagcg ggcgtgggcg gttgttctcg tcatagaccg gctccaacac
     9061 ggccacccca tacatcttga tcgagaagcc gcgcatacca acggccgcca ggtggccgcc
     9121 catgtcgcgg tagtgcgggc cggcgaagtg atcgtgcagg gcctgttcgc tgtcccattc
     9181 ctcgaacacc tgcacgcgcc cgggcaccag cggatcgaag gcccaggtat aggccacgca
     9241 gcccggctgg ccgcgcgcgc cctcgatatg ggcgcgcgcc tccagcagca ccgccgggca
     9301 atcggccgga tcgaaatcga tgtggccggc aatggtcacg ctgttgatgg ctgtcgtcat
     9361 ggtctgtcct ctcctgccag cggcctgatc tggcgcgcag ggtggcggca aagccgcggc
     9421 cgatcaacct gcccaggtgg caggcccccg cccgccccta gccccgcgcc ggatcggtga
     9481 tcgccggcca gggcccggca aggcagccgg ccagccgtcg ctgccagccg ccggggccgg
     9541 tggccagcag gtcataccag ccgcaactgg cggccagcgg ccagtccatc gtgccgcctt
     9601 cggggcccgg cgcccagcgg ccgtgcatgg ccgcataggc ctgcggcgcc accaccagcc
     9661 cgccatggcc gggcgccacg ctgaggcgca agcggtcttc cagcagcgcc cagcgcagct
     9721 ccggctctgc cgccgcgccg cccacgaaat ggcggtggaa cccccagggc cccagcaccc
     9781 acaggtcgta ggcgcgggct tcgccatgca gcggccattc gtcctcgatc tgccggcccg
     9841 gctccacggt gaagcggcgc ggcacaaggt ccagccgcag ccggtcgtag acatggaaca
     9901 cgccggcaag gcccgcccct gcggcaaacg ccagccgcag cgcccgcccg cccgccgcca
     9961 gcgcgccgcg cacgtccagg cgatagcgcg ccgagcaggc cggcttcacc cccggcaact
    10021 gggccggcag cggctgggcc agcggcggcg ccggatcggg ctggcgggcg atggcgcggg
    10081 cccgcgcggc ccgccggggc gcggccgcca ggctttcggc aagcgccggt ggcagccctt
    10141 gcggcacccg cgccgcgccg gcaaaatcga atgccgtcgt gagatcggag caaaccgccc
    10201 ggcgccaggg gctgatatgc ggctcgcgca cgccaaaccg cgcttccatg aagcggatca
    10261 ccgaggtgtg atcggccact tcggaatgga tccgcccccc gcgcgaccaa ggcgagagca
    10321 catacatcgg cacccgcggc cccagcccat aggggcgctg gcgcagttcg ggatcatcgt
    10381 cctccgcgcc gggggcgggc aggcggtggt attcgccctg tgtgctggcg gtgctggcgc
    10441 cggcaaagcc gccgggcaga tcggcatcgg gcgaaggggg ggctggcggc ggcacatggt
    10501 cgaaaaagcc gtcgttctcg tcgaaattga cgaacaggac ggtgcgcgcc cagaccgccg
    10561 ggttggccgt gagcgcggcg atcacctgcg cggtgtaggc cgcgccctgc gccgggctgg
    10621 aggggccggg gtgttccgat ccttccgccg tgccaacgat ccagctgatc tgcggcaggc
    10681 gatcggccag cacatcggcc cgcagggcat cgagcgcgcg ggtggccagc cccttttgcg
    10741 ccaacacccg ccgggcgccg gcatcccccc gccaggcggc gcgatattgc aggaaattgg
    10801 ccagcggatt gtcgccgaaa ttatcggcca tatcctgata gaccgcccag gacacccccg
    10861 ccgcctccag cctttcgggg taggtcgtcc aggaaaagcg cggggtgctg gcggcatcgg
    10921 gccggttcag gtcgtcctcg gggttgtcca ccgccgggcc gccgccgcgc ccgcaagggt
    10981 cattcgtgcc ggtccacagg aacagccggt tggtgttggt gccggcatgc agggcgcaat
    11041 gataggcatc gcacagggta aaagcctctg ccatcgccca ttggaagggg atgtcggcgc
    11101 gggtgtaata ggccatggcg cgctgggtct tggcctcggg ccagtgggcc atgcggccgt
    11161 tatcccaggc gcgctgggca ttggcccagc tgtgcggcgt gccctgcatg cgcatgagat
    11221 cgaaatcggc gctggtgacg agcgggaagg gacgcatccg ccggggcgga tcggccgcac
    11281 catcgcgctg gtcccacacc tgcaggggcg cgccatcggc cgccgccggc accggaatgg
    11341 ggaaccggtc ggcaaagccg cgcaccccgg gcatggtgcc gaaataatgg tcgaagctgc
    11401 ggttttcctg catcagcaca acgatatggc caacatcgcc cagcgtgccc cgccgccggt
    11461 ccgggccaat cgcggcggcc cgcgccagca ccgcgcccag cccgcccggc agcgccgcca
    11521 gccccgcccc gcccatggcg ccccgcagca gcccgcgccg gccgatcatg gctgcgcccc
    11581 tgcggcgatg ggggccagcg gcagcaggcg cgccgccagc gcccggcgca gcagcggtgc
    11641 cgggcaagcc agcgcctgcc ggccacacag gccgatgggc agcgccattt cggccgggcc
    11701 gtggccgccc tccagcgcct gcagccggcg cacctggccg ggccttgccg ccacatagaa
    11761 ggcgcgcacc atctgccggc catcggcccg gcgcagcagc gccagcccca gcgcgccccc
    11821 cggcggcgga tcgttccagc ccagccccgg cacgcggaaa cgggtgcgca gcagggcgcc
    11881 catcgcggcg atgttgttgt catggcccac cagcagcgtc agccggggct ggccggcatc
    11941 gaacagcgcc gagagcatcc ggcgggccag cacgctggcg ctgcgcgggg ccatgtaggg
    12001 ggatcggtcg aacacatcga acagggccgc atgcagcggg ctgagcgcgg cgatcaccgc
    12061 cgggcccgcc ctgccccagc ccacttcggc aggagcaaag ccttccatat attggagaag
    12121 gaagatctgc gcggtgcccg atgcctgctc taccgggccg ctgaaggcca ggctgcggcc
    12181 atctgccgcg ggcgcaatgg cacctggcag ggccgccagc cggcagggcg ggcgggcccg
    12241 gtcgcagccc agcacctggg ccatgcggcg cagcgccggg gcataatggc ggg
//
LOCUS       DASRYE010000256        43396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_15910,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000256 DASRYE010000000
VERSION     DASRYE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 43396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43396
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15910"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFF98_13030"
     CDS             complement(<1..678)
                     /locus_tag="VFF98_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZU64598.1"
                     /translation="MLALATIRPGEPELFAALQGEGASIGRPSVFVRLSRCNLACRWC
                     DTAYTWRFEGDNRPHTDDAHFSRADNQLMLEEEEVAQRIRALGGHWPEPRLVITGGEP
                     LLQGPALARMLAELPGWHVEVETNGTVAAAPALDPLIHQFNVSPKLAHSGNPAELALL
                     PARLAAYAADPRAFFKYVIATSDDIAEVDALVAAHAIPPARVFLMPEGRDAATLHARM
                     AWLADACL"
     gene            complement(680..3394)
                     /locus_tag="VFF98_13035"
     CDS             complement(680..3394)
                     /locus_tag="VFF98_13035"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZU64599.1"
                     /translation="MTEDFAQPTLDKTFDPSGLEAKWYAHWEEGGLFRPERPDAAPFT
                     IVNPPPNVTGSLHIGHALDNTLQDIVIRYERLRGKDALWVVGTDHAGIATQMVVERQM
                     EARGDKRTNYTREGFIDKVWEWKAESGGTITRQLRRLGCSMDWSREQFTMDPHFTRAV
                     VKVFVDLYNQDLIYRDKRLVNWDPKLKTAISDLEVETREVKGGFWHFKYPLADGVLRD
                     DGLDYIEVATTRPETMLADMCVAVNPEDERYQSVIGKEILQPITGRRFKIVADEHADP
                     ELGSGAVKITPGHDFNDFEVGKRAGIAPNDMLNMFDADANVVQTADGLIPAEYLGVER
                     FEARRMIVAKMKELGFLIPHVTKDKEGNETEHDAEPRTIQTPFGDRGGVVIEPWLTDQ
                     WYVNAGVLAQKPLEAVKSGAIEIVPKAWEKTFFNWMENIQPWCVSRQLWWGHRIPAWY
                     AEDGSVFVAETEEEAQGLAGNKALTRDPDVLDTWFSSALWPFATLGWPDENAPLFQKH
                     YPNDLLVSGFDILFFWDARMAMQGMHFLPGVPWKRLYLHGLVRAADGQKMSKSKGNVV
                     DPLGLIDQYGADALRFFMAAMESQGRDVKMDEKRVEGYRNFATKLWNAARFCQSNGIV
                     GSASLAAPVATSAVNKWIIGEVVETLAALDRAMAELRFDAAAHAIYRFVYDGFCDWYI
                     ELIKGSFDAETRAVAGWVLDQILVMLHPFMPFVTEELWSALASPDAPRANELIVARWP
                     APEASVDAEAKAEVEWLIALIGNLRGAKVELGLAPGARLEAWLPEVSEATRAIIARNP
                     AAIDRLARLSAIHFAPAPAGAAMQVGAGDANLIVPLEGLIDVAAEKARLAKALAASQK
                     ERDSLDKRLGNPAFVEKAKPEAIDKARADHAHHAAEAERLAAALARLG"
     gene            3518..4630
                     /locus_tag="VFF98_13040"
     CDS             3518..4630
                     /locus_tag="VFF98_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64600.1"
                     /translation="MDQLPDFTIKTDDSGAVELALAGPLVVARIAALDKALRALAGPV
                     ARLDLAGVTAIDTVGAWLCWRTADALGAEITHADEDSRRLINAVRGDQEELPPPPPPQ
                     PAWRETLARLGQLVVDGATGAVDVLAFMGALLEAAWGIARHPGRLRGTALARQLQLVG
                     IDALGIVGLMSFLIGIVIAQQGAVQLQQFGAQIYTINLTGRLAFRELGCLMTAIMVAG
                     RSGSSFAAQLGTMKLTEEIDAMRTIGVSPLEALVLPRVLAAVWMLPLLGFYSALIAIV
                     GGASISAVVLDIPFVTFLLRIQEVVPIRDLWVGLIKAPVFGFIIAITGCYQGLRVSGN
                     SEEVGLRTTMAVVQAIFMVIVLDAFFAVFFSGVGWG"
     gene            4627..5478
                     /locus_tag="VFF98_13045"
     CDS             4627..5478
                     /locus_tag="VFF98_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU64601.1"
                     /translation="MNEALATAGDCPIRIEGLENRFGEHVVHENLSLSVRRGEILGVV
                     GGSGTGKSVLMRSIIGLQHPSAGQISVLGHDICRADPAGNADLRSRWGVLFQGGALFS
                     TLTVAENVEVPLKEFYPDIDPALRREIARFKVRLSGLPAEAADKYPSELSGGMKKRAG
                     LARALALDPELLFLDEPTAGLDPIGAAAFDKLIRALQATLGLTVFLITHDLDSLHAIC
                     DRVAVLADKRVIATGTIPELMETHHPWIEEYFNGPRGRSALLSRAGDAAPPTATAALD
                     RPGKPKA"
     gene            5487..6425
                     /locus_tag="VFF98_13050"
     CDS             5487..6425
                     /locus_tag="VFF98_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZU64602.1"
                     /translation="METRANHVWVGAVTLALLALIAAAAIWIARVSEGVKNDYDIFFR
                     QSVEGLGRGSAVTYAGVPAGKVVEIELWKKDPSLVRVRIAVDDRIPILAGTTASLQGS
                     FTGVSTIQLNDGVKGAPPITAPGPEGVPEIPTKRSGLGALLSNAPVLMDRLTSLTERL
                     GQVLSDQNIAHIDGVFANTDKMTRGLAEATPQLRTDLADLDATLQQATQTLTAFQKVA
                     DSANAQLDPNGPSLVHQMRQTLKSAQAAADALHGELDEARPATHQLATSTLPQTEATL
                     RDLRETSRALREITEKIDNQGASGLLGAPKLPEYKP"
     gene            6464..7021
                     /locus_tag="VFF98_13055"
     CDS             6464..7021
                     /locus_tag="VFF98_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZU64603.1"
                     /translation="MFFLGLSGCVSLGGGKAPERLFTLRSDAVAASGQTVSASSAEAV
                     LVVEPDTDRSLALQRVPVQVDANAIAYLKDANWVERPARLFRTLLAEHIRAGGKRLVL
                     EEDGALPAGAMRLGGRLTAMGYDARSHSVVVRYDALRDEGGGKLSARRFEAVEADVPA
                     QAHAVGPALNRAANKVASDVAAWIG"
     gene            complement(7025..7720)
                     /locus_tag="VFF98_13060"
     CDS             complement(7025..7720)
                     /locus_tag="VFF98_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HZU64604.1"
                     /translation="MKRFYKDVSTAAADGGWRVLLDGRPIKTVGGAPQIAPTQALAAA
                     LADEWAAQGEKIDAAALPLRDMADYALDVIAPDRAAAQAALIPYGETDTLCYRADEGS
                     ALLDRQIAVWEPLLNATEARYDIHFERVSGIIHRPQPAATLARLQAALAAEGDFALAA
                     LRNLVSLAASLVVGLAALEPGADVAALWQAACLEEEWQADLWGRDAEAEARRARRTAD
                     FARAARFAALARG"
     gene            complement(7747..8409)
                     /locus_tag="VFF98_13065"
     CDS             complement(7747..8409)
                     /locus_tag="VFF98_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZU64605.1"
                     /translation="MASSRLAVFDCDGTLVDGQADIIAAMDEAFARLDLPARPAQAVR
                     RIVGLSLPAAMARLAPEADAQLVADLVEAYKAAFRRARQEGRLAEPLFPGIRALVDEL
                     RAEGWQLAIATGKSDRGLAHCLAAHGLESHFDSLQTADRHPSKPHPAMLEAALAECLA
                     QPHQAVMIGDTAFDIAMAEAAGVRAIGVAWGYHPPAELLAAGAVGVANSVRELGEMLR
                     AG"
     gene            complement(8409..9785)
                     /locus_tag="VFF98_13070"
     CDS             complement(8409..9785)
                     /locus_tag="VFF98_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZU64606.1"
                     /translation="MNETEHKVRQFVVGRDDDGIRLDRWFKRHLPQVGFATISRWART
                     GQIRVDGKRAAPEDRLVAGQTLRVPPGDSHAPGGAGKAPRARKPLTEAQIELADSMVI
                     HRDPAAIVLNKVPGLATQGGSGTYEHVDGLLDAYATGSTRPRLVHRLDKDTSGVLLVA
                     ATPGSAAFFSKRFSGRQARKIYWALVAGVPSIDEGLIDLPLAKQPGSGGEKMMVDQSE
                     HGQPSKSRYRVIDRAGNRACWVELQPLTGRTHQLRVHMAAIGHPIIGDGKYGGPEAFL
                     SGSISRKLHLHARRLIIEHPDGTPLDVTAALPDHFAASLAQLGFEESEGEYIPDPSGD
                     FSREEQKSAARAHAKQYRKERRGERRGRADTGRSRNDATHGGGHHGGGHQGSGQNAGP
                     AARKGPRKPGSPKPGGPRPGAPKSGGKPASARPDSSRSGRPGGHAGHPGKGAARPARP
                     GGAPRGKR"
     gene            9965..10663
                     /locus_tag="VFF98_13075"
     CDS             9965..10663
                     /locus_tag="VFF98_13075"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64607.1"
                     /translation="MRSIHTPKIDAAYWLAIVLASIAGTNLGDLYADRSGLGIFGGLW
                     LLVALAAAVFVAERRDRRAHELWFWLVILIIRTGATNIADGTAHLWLARPDLSALTAV
                     LLAVAAGFSRWAEPGRSLKADAPFWLAMLAAGVFGTAAGDVAQHLLGDWQACCVLGAA
                     LAGAVALWVRVRSLAAYWLAVALARTAGTAIGDVLAEGAVDIGIIASTALSLPLFALV
                     AGWGQRRRALAAAA"
     gene            10852..11058
                     /gene="rpsU"
                     /locus_tag="VFF98_13080"
     CDS             10852..11058
                     /gene="rpsU"
                     /locus_tag="VFF98_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZU64608.1"
                     /translation="MQILVRDNNVDQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     REKAAAVRRARKLERKRAERDGAK"
     gene            11244..11789
                     /locus_tag="VFF98_13085"
     CDS             11244..11789
                     /locus_tag="VFF98_13085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZU64609.1"
                     /translation="MTEITRVPLQPLTKGSIKKLWLGVVAASLAGAAIAGAGMPPLVS
                     VKTLTPGFGPSPSGDALVQINYVGKLANGTVFDQGKGAVLPLQGTIPGFAKALAQTQK
                     GGKYVFRIPARLAYGPIAQGPIPANSDLTFEVEVLDFRSRAELEAQQRMMEQMMRAQG
                     AHQQAPQAGDAAPAPQAAPAQ"
     gene            11990..12292
                     /gene="gatC"
                     /locus_tag="VFF98_13090"
     CDS             11990..12292
                     /gene="gatC"
                     /locus_tag="VFF98_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZU64610.1"
                     /translation="MSVTTEEVARIAALARLKVTQEELAAMVPELNGILAWVEQLGEV
                     DVTGIAPMTAVIPNHLRLRADVVNADPLTGGGIRDAVLANAPAAEHGFFGVPKVIE"
     gene            12292..13776
                     /gene="gatA"
                     /locus_tag="VFF98_13095"
     CDS             12292..13776
                     /gene="gatA"
                     /locus_tag="VFF98_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZU64611.1"
                     /translation="MTELTELTLAGIRDGVAAGTFSATEVAEAFNTAVADAQEALNAF
                     IVTTPEAALEAAKKVDADRAAGRPLGKLAGVPIGMKDLFATKGVQTTAASHMLEGFIP
                     PYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSHFGNVISPWRRPGETTPLAPGGS
                     SGGSSAAIAARLCPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQ
                     AGPMARTVRDCAIMLEAMAGFDPKDATSLDLPVPAWEAGLNPDLRGKKVGIPREYRIE
                     GLNAEVAKSWDDGIAWLKDAGAEIVEISLPHTKFALPTYYIIAPAEASSNLARYDGVR
                     YGLRDLPEGANLQEMYAATRAAGFGGEVKRRILIGTYVLSAGFYDAYYTQAQKVRALI
                     AQDFLKAFEEVDVILTPTAPSTPFALGEKRADPVEMYLNDVFSVPASLAGLPAMSVPA
                     GLASDGLPLGLHLIGKPFDEAGVLNAGLAIEARAGFHASPAKWW"
     gene            13773..15275
                     /gene="gatB"
                     /locus_tag="VFF98_13100"
     CDS             13773..15275
                     /gene="gatB"
                     /locus_tag="VFF98_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZU64612.1"
                     /translation="MNMSKYRIQGATGEWEVVIGLEVHAQVTSGSKLFSGAATAFGAE
                     PNSQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIEAEINKWSRFDRKNYFYADLPQGY
                     QISQLYHPLVGEGQLTIEADEKAGLPEAKQIGIERIHVEQDAGKLMHDQHPTMSYVDL
                     NRSGVALMEIVSRPDMRSPAEAGAYLAKLRQILRYVGSCDGNMDQGSMRADVNVSVRR
                     PGEPFGTRTETKNVNSVRFVMAVVESEAHRQVALIEDGGTVAQETRLYDPDRNETRSM
                     RSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRARYVDGLGLSAYNAHV
                     LTAEVDTARWFEALLAATARAQGKGEAEVAKQASNWLTSEFFGALNKLGKDLATSPVS
                     PEGAAELLALVGDGTISGSIAKQVLEKMLESGEGAGAIVEREGLKQTSDTGAIEAVIA
                     DVLAKNTDKVDQYRGGKEALFGFFVGQTMKAMGGKANPQLVNELLKKALG"
     gene            15377..16558
                     /locus_tag="VFF98_13105"
     CDS             15377..16558
                     /locus_tag="VFF98_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64613.1"
                     /translation="MAIWRGGMGMLAATALLGAGVPAMASRGAPAASRSAAPTSDTAE
                     DEDADTDTSAPMSGEDLTNAQVVALNRTRLGDPALAQMVRVCKPHFDISTRALLALRA
                     AGVSSRVIAAMIKVQAKADGTREDNDSPDPHQPHGAGLYLLAGWLEAPRMFALAPQTT
                     TRTTSGSILGYAFSGGIVSVGYKAMLPGAHAPVIAEGARPVFYVFGAKAGNAWGPVPA
                     PEEVSLVRFSQGAHGREVRIGSFNIHGASTGIRDADRIAFRIRQLGQGAIELRPEEDL
                     APGEYGFVQTAAGVGIGAARASTSSARVFAFAVGPHGSQPAQLPQPGTSVQRGPGMDR
                     LNVRAGRRTAAVSGMEGGAGNTGQQSNASQPGNTGQPANARKSGQPASGAEWGDSGWA
                     Y"
     gene            16648..19545
                     /locus_tag="VFF98_13110"
     CDS             16648..19545
                     /locus_tag="VFF98_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZU64614.1"
                     /translation="MLYSTRLSKIARPAARFSSASLIALCLAGTSLAGTAPSAPAAPP
                     AAPLADLVAQVNIPFERFQLANGLTVIVHTDRKAPIVGVTTYYRVGSKNEPKGHTGFA
                     HLYEHLFFGGSANAPSFDEPLEQAGATATNGSTSYDRTNYVETVPKGALDLALFLESD
                     RMGHLLAAVTQDKLDKQRGVVENEKRQDDNQPYGLLSYALAEGLFPVGHPYRHTPLGS
                     MADLDAATLGDVRAWFTQHYAPGNVVLVLTGDIDAATARPLVERYFGAIPAGPKVEPV
                     AAGPVTLAAPRARAMTDRVATTTLYRAWSGPGVNDPDAEALEIGMTVLGGLASSRLDN
                     ALVRGRQLAVGVTASDEQNEQVSQLTASLSIKPGVPRAEAEAAFDAEIARFLAEGPTA
                     DELRRAATRTVAEEIGALEVIGGFGGKGAQLAEGQLYSGDPAHYRAELARIAALTPEA
                     VRDAMRRWLGRPAYNLTVNPGERTEKGDAMGGWGDEAATPARPVDAKKPVPPIAAGAP
                     RPQPAVAPVGALAFPAVEHARLSNGMAVDLARRTAVPKVLVSLGFDAGVAADAVDAPG
                     TQGLLLAMLQEGTAGAHPRDATQVLEEQERLGATISAGASLDTSTVTLDALKANLAPS
                     LGLLADVLRSPAFAAGDLARVKQQRLTALEQTLASPQGQAARALNPLLFGPGTPYGQP
                     GDGLGSKASLAALDGPALRKAMGEWLRPDNARITIVGDITMAEALPLLERAFGDWAPP
                     AAARPAKPDVAPPPPAPRIVVIDRPHSPQSVILGGRVLPLSGRDAVMEPLGVANDVLG
                     GDFLSRLNTDLREEKGWSYGVSTTIRQPLGRASLIVYAPVQTDRTGDAIKALVADMKA
                     LPGRQPVAPAELARATDGTIRQLPARFETNGQVLGAVSTNQRYGRPDDYYVTLPQRLR
                     GVDAKQADALAARYLQPDGLVFVVVGEKAAIAPQLKDLGLPVEYVSGGAGE"
     gene            19624..22203
                     /gene="clpB"
                     /locus_tag="VFF98_13115"
     CDS             19624..22203
                     /gene="clpB"
                     /locus_tag="VFF98_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZU64615.1"
                     /translation="MNLEKFTDRAKGFLQAAQVVAIRNNHQRITPEHLLKALLEDEEG
                     MAAGLIQRAGGTPAFAVQEVDKALAKQPAVTGSGAQNSPGLDNEAVRVLDQAEQLSAK
                     AGDAFVPVQRLLQALALATTTPAGQALKAANVSPQALETAIQELTGGRHADSASAENA
                     YEGLKKFARDLTEAAREGKLDPVIGRDEEIRRTIQILARRTKNNPALIGEPGVGKTAI
                     AEGLALRIANGDVPDSLKHRKLMALDMGALIAGAKYRGEFEERLKSVLDEVKGAEGEI
                     ILFIDEMHTLIGAGKTDGAMDASNLLKPALARGELHCIGATTLDEYQKYIEKDPALQR
                     RFQPVFVGEPTVEDTISILRGLKEKYELHHGVRIADNAIVAAATLSNRYITNRFLPDK
                     AIDLMDEAASRIRMEVESKPEEIETLDRRIIQLKIEEMALSKESDEASRERLATLREE
                     MANLEQRSAELTTRWQNERDKIAAEGRIKEQLDAARVEMEQAQRGGDYAKAGELAYGR
                     IPELERQLAEAQGASANALLREEVTAEDIAGVVSRWTGVPVDRMMQGEREKLLHMEDE
                     LGKRVIGQRDAVVAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALAGFLF
                     DDDTAMVRIDMSEFMEKHAVSRLIGAPPGYVGYDEGGVLTEAVRRRPYQVVLFDEVEK
                     AHPDVFNVLLQVLDDGRLTDGQGRVVDFTNTIIILTSNLGSQFLANLEDGQTAESVEP
                     QVMEVVRAHFRPEFLNRLDEIILFHRLGQEHMGPIVEIQVNRVQKLLKDRKITLSLTH
                     AAERWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLGGEIPDGSTVAIDEGDGALSI
                     AVS"
     gene            22414..23055
                     /locus_tag="VFF98_13120"
     CDS             22414..23055
                     /locus_tag="VFF98_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZU64616.1"
                     /translation="MTGQPSLAAPTPVPAAAREQLLDTASAIMRESDTVDVSLSELAA
                     QSGLNSALVKYYFGNKAGLMRALLQRDFDAILQAVDTLMSRDMDPEAKFRRHLTAVVD
                     TFFATPYIHRLLMRLIREEPPEEAQRLADTYLKPLLNAYEKLISDGVAAGVFRPVDPQ
                     LFYFTVIGAADRFFSARLVLRYSSDTDVLDEALRDRYRAHLNDFVVAGILVPR"
     gene            23158..24036
                     /gene="mtnP"
                     /locus_tag="VFF98_13125"
     CDS             23158..24036
                     /gene="mtnP"
                     /locus_tag="VFF98_13125"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZU64617.1"
                     /translation="MSTTGWHIGIIGGSGLAAGLDLDDAREVIVESPFGAPSAAIVQG
                     SLNGIRFSFLPRHGKGHATPPSLVNFRANIDSLKRVGVTDLVALSAIGSLREEYAPGH
                     FVTVDQLIDRTHGRPSSFFGEGLVAHVSLADPTCPRLSGLMALAVQHAGGIVHPRGTY
                     VAIEGPQFSTRAESRMYRHWGADVIGMTAFPEARLAREAELPYALVGMVTDYDAWREE
                     EAGVEAAEVFAVMQANAALARAAVRELAQVLPPERAASPFDTALEHAFATPAAARDPA
                     LVARLDAVAGRVLRQG"
     gene            complement(24076..25077)
                     /locus_tag="VFF98_13130"
     CDS             complement(24076..25077)
                     /locus_tag="VFF98_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZU64618.1"
                     /translation="MIQHPAAPHLFGVDVFWVGTLLAMVAAGAVLVAIYAALTVRDPM
                     AKRVKALNERREELKAGIMSSGPKKRAQIVRRSDTTDTIRDILNKLKVLQDSQLAEVQ
                     QKLAQAGIRNKEWGVAIIFGRMVVPIVLGLLVVICVYWLNLWPEWSPFKRFIIFAVAT
                     AGGYKGPDLYIDNLVSKRTTAVRKGLPDALDLLVICAEAGLTVDAAFGRVARELGRAY
                     PELGDEFALTAIELSFLAERRQAFENLAYRVKLDHVRGVVTTMIQTERYGTPLASALR
                     VLSAEFRNERMMRAEEKAARLPAIMTVPLILFILPTLFVVILGPAACSIADAFSHKS"
     gene            complement(25091..26062)
                     /locus_tag="VFF98_13135"
     CDS             complement(25091..26062)
                     /locus_tag="VFF98_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZU64619.1"
                     /translation="MNMMQILMLAVGGMLVLGLVGFAFLGPDAGKQGARRLQAVRFRH
                     SDSAADKMEAQLRKALASRKPAYTTAAAQISRWAALALRLHQTGKHWTVEQYLYTCAG
                     ITAVIGLLVYMRSGVPLLALAVGVAVGAGVPHMVVGHLIQRRANAFISKFPDAIELLV
                     RGLRSGLPVTETLGIVSREVRGPVAQEFKLVTERIRIGRTMEEALQETADRLSMAEFN
                     FFCITLAIQRETGGNLAETLANLADVLRKRAQMKLKIKAMSSESKASAYIVGSLPFLV
                     FGMIYWINPEYLGGFFTDQRLIVVGLGAFVWMGTGAFIMAKMVSFEI"
     gene            complement(26088..27368)
                     /locus_tag="VFF98_13140"
     CDS             complement(26088..27368)
                     /locus_tag="VFF98_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HZU64620.1"
                     /translation="MNAPWKAGGTSHSARDTFAAFLCDDASLDVLRPLSAEMGWPPER
                     CVKGGLRNAIQTLSVSSSPSILMVDLSESGDPLSEINHLAEVCEPGTVVIAVGQVNDV
                     RLYRELLTSGIHDYLLKPLVASHVRETLQHAQAVFAAPRNQDPSAAHAHVSTAVIGTR
                     GGVGASTIATAMAWLFASDHQLPTALFDLDIHFGTGALALDLEPGRGLTDAIENPSRI
                     DGLFLERAMIKAADNLSILSAEAPISAPLMTDGSAFIQLEEEFRQSFEMTVVDLPRSM
                     LVNFPHVLADVNVIVLVTELTLAGARDCIRILSWLRANAGHALVLVVANKVYSGLSEI
                     GRADFEASIERKINYVVPFDAKAAIHAAKLGQTFTEANKASKATAVLRELAQAVRANA
                     SDSTAPVEVPEAKSLLGLIDFKSLLARKDKAAGR"
     gene            complement(27370..28053)
                     /locus_tag="VFF98_13145"
     CDS             complement(27370..28053)
                     /locus_tag="VFF98_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HZU64621.1"
                     /translation="MPATPALPFSRHSRRALALVLPLSLSLALSACIGGQPRNRLLDS
                     IHQPVVHHTVYTLDLQSGPNGLGMAELQRLSGWFAALKLRYGDTVAVDDPVANHATRA
                     SITSVLTRYGLPLAEKAPVTEGYVPTGSVRVVITRAEAEVPHCPDWSANSETNFKNAT
                     SSNYGCAVNGNLAAMVANPDDLLHGASNAGETTQVSGDKAINAYRTSAPSGGGGKNVK
                     ASATSGGGS"
     gene            complement(28068..29810)
                     /locus_tag="VFF98_13150"
     CDS             complement(28068..29810)
                     /locus_tag="VFF98_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZU64622.1"
                     /translation="MIRRLPRIACAHRLLACTIAAIGLLPTTALAQEEGAGVLLARNA
                     PAHRPGAHQPARRPLAHRPAPHAGVRLVAGRTAPLGAASITRPASEILLSIGRGQLVN
                     LGGTVSDVFIADDKIADVQVKSNRQLYIFGKGAGETTIYASAPGGQIVWSSTLRVGSN
                     IDSVDQMLKVAMPDARITVSTMSSNTVLLTGTINAPEDSAEAQRLVQAYMGSGTNVIS
                     RLHTATPLQVNLQVRIAEVSRKLTRALGVNLQTVSSSNGFAFLTGRSGLYSTGTSSTS
                     SSSSSSTSSASASSGGTSVTLNGLASSNTLGYLTSFGGMTLLSALDAAETVGLVTTLA
                     QPNLTALSGETADFLAGGQYPIPISSGLGTTSIEYKNYGVSLSYTPTVLADGRISLRV
                     RPEVSQLDTSNAVTMNGTSVPALTIRRAETTVELGSGQSFMIAGLLSNSATNSISKIP
                     GAGDVPVLGALFKSTSFARGDTELVIVVTPYLVKPVNDGDIKLPTDGMQSPDDIQRVF
                     GNMLADRPKDPKDAKRPGATAAESGGKPAIAAPAPQSSAKSPAPPAARTGDAAPATST
                     PPAAAPGQPGFSLN"
     gene            complement(29807..30955)
                     /gene="cpaB"
                     /locus_tag="VFF98_13155"
     CDS             complement(29807..30955)
                     /gene="cpaB"
                     /locus_tag="VFF98_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZU64623.1"
                     /translation="MDQRKLMLLLGAMVIAIGTALAARSLLSGSAAPQVAAALPAAVA
                     PKGPKVLVAQRALPVGTIITADAVSFQEWPKDMVRDAYFVEGSVAMNKLVGTVVRFPI
                     TAGQPLTQGALVAPGDRGFLAAALGPGMRAITIPVSDKTSVAGFIFPGDHVDLMLTSS
                     VKDSVQMTAANVSVSDDRPVYATETILRNLRVLATDQATETESANGKTVVHPFHNVTL
                     EVTPKLAEKIEVAQSVGTISLVLRSIADNQSDLDHAVASGQVKLKDGASPQEESRLIT
                     AAMNRPSQGATSFSTGGDVSRFQRHAGPNTGKTMSSSTPQGQAAGMMRRGNGVTVTRG
                     RDTTEVQTGRAGLYGGNAGPYTPTATIGMGVGNAGNAAGNALPAGAMQ"
     gene            complement(31069..31557)
                     /locus_tag="VFF98_13160"
     CDS             complement(31069..31557)
                     /locus_tag="VFF98_13160"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZU64624.1"
                     /translation="MQVGAIAGGLLAALALALVVASITDIRRRQIDNKLNGAIALAAP
                     LYWWAAGLGWPAIGWQLGASAATLLFFGLMFARGYMGGGDVKLLAALALWIRPDRFLN
                     LLVAMALAGGVLTLAMLIRHRLGARTEKLEVPYGIAISAAGLWILAIAYLPLLRAGAA
                     LG"
     gene            31737..32846
                     /locus_tag="VFF98_13165"
     CDS             31737..32846
                     /locus_tag="VFF98_13165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64625.1"
                     /translation="MNRPTLPAGDSAGLLQPYDIALIGAGMAGASLAAALLARAPALR
                     VVLAEAEARPGLHATGRSAAFWTESYGGPGVVPLTLASHAALEGLGVLRRRGALTLAR
                     AGQEAQLEAFAARYRALGVSTGVLGRAQAAALVPGLKPGWIGGVMEPDCCDIDVARLH
                     QHYLAEARRAGADLLCGAGLASAEPAGAGWQLHLADGRRLRAGVLVNAAGAWADEVAR
                     RAGAVPLGIQPYRRTMAQLRVAPQPDADLPLVLDLDESFYFKPQSGRLWLTPHDETPS
                     AACDAAPEEIDVASAIARLEHRWAGLRSFAPDRLPAYGWDGRVPGFFWCAGQGGFGIQ
                     TAPAGADLAARLLLGEGPGAVNPVPYDPRRFALVD"
     gene            complement(32843..33283)
                     /gene="rnhA"
                     /locus_tag="VFF98_13170"
     CDS             complement(32843..33283)
                     /gene="rnhA"
                     /locus_tag="VFF98_13170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZU64626.1"
                     /translation="MKDVEIFTDGACKGNPGPGGWAALLRMGEHEKELVGSEAETTNN
                     RMEMTAVIRALAALNQPCTVRLTTDSKYVIDGITKWVFGWQKKGWVNAKKEPVANEDL
                     WREMLLVARPHKVDWQWVRGHNGHAENERVDKLASDAAAKASRR"
     gene            complement(33280..34275)
                     /gene="thrB"
                     /locus_tag="VFF98_13175"
     CDS             complement(33280..34275)
                     /gene="thrB"
                     /locus_tag="VFF98_13175"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZU64627.1"
                     /translation="MAVYTHLDDSAFAALIAAYDVGAPLMHKGIAEGVSNTNFIVETT
                     GGPAGGPRFILTIYEARTEVADLPFFLGLLDHLAARGCPVPRTIHDRQGALSHAVAGA
                     DGGQKPAALIEFLPGVSLSRPTPAQARAVGQALARVHLAAADFPMARANGMGLATWRD
                     LLDGCGAAGLAAIDPALGDLVACELPALAAHWPDHLPTGIIHADLFPDNVLMLGEDVT
                     GLIDFYFACTDTLAYDVAVTHAAWCFSADGRQFDAGISAALLAGYEAVRPLSAEERAA
                     LPLLARGAAMRFLATRAYDWLHTPADALVTRKDPMAFARRLEFYADPAHAGLFAA"
     gene            complement(34287..35255)
                     /gene="ispH"
                     /locus_tag="VFF98_13180"
                     /gene_synonym="lytB"
     CDS             complement(34287..35255)
                     /gene="ispH"
                     /locus_tag="VFF98_13180"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZU64628.1"
                     /translation="MNAGFPSPSLPPLRLLVAAPRGFCAGVDRAIEIVERAIRKYGAP
                     VYVRHEIVHNRFVVDGLREKGAIFVEELDEVPDGAPVIFSAHGVPKSIPAKANERGLD
                     WLDATCPLVSKVHRQAERHIEAGRHIFFIGHKGHPEVIGTLGQVPEGQMTLVETLEDV
                     AALPFEDNGKLAFLTQTTLSVDDTAEIVAALRARYPQIAGPRAEDICYATSNRQAAVK
                     AIAEDCDAVFVIGAPNSSNSQRLVEVAERFGTRSRLVQRASEIDESWLEGVNTLGLTA
                     GASAPEELVREVVDLISTWRSVTEETIITAEEKMIFKLPRQLTDAE"
     gene            35327..36016
                     /locus_tag="VFF98_13185"
     CDS             35327..36016
                     /locus_tag="VFF98_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64629.1"
                     /translation="MQFRSRLVFALATGALLAGCSQKGELVVDEGVGIAAIRSQCPSV
                     GIADYTGDVTTFRAPGATDTANMDVSAALTNVRMKCDQDGVKLDTRQAEVRPGELVHA
                     TVSFDVLARRTDGTGARDLNLPFFITVLRGSNAVIAKRIGTVAIHFAPGELRATGHAE
                     GLALIDKNEATLSRDIRDRITKKRVAGEADAAVDPLAQSDVQDAIARATFEVLVGFQL
                     SDEQLTYNATR"
     gene            36086..37846
                     /gene="argS"
                     /locus_tag="VFF98_13190"
     CDS             36086..37846
                     /gene="argS"
                     /locus_tag="VFF98_13190"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZU64630.1"
                     /translation="MSDSQALSAAPVFTAFAGHVAAALDRLEAAGTLPAGLGRAAVTV
                     EPPRDPAHGDLATNAAMVLAKPAGTNPRALATALVAELEKLLEVASASIAGPGFINLT
                     LAPAAWVEEIRKIAALAGDYGRSALGGGTTVNVEYVSANPTGPMHMGHCRGAVVGDAL
                     ASLLEFAGHKVIREYYVNDAGAQVQVLARSAHLRYREALGEAVGEIPAGLYPGDYLVP
                     VGRALAEEFGDRYARAPEAEWLALFRVKAVAAMMAMIRADLALLGIRHDLFSSEAELQ
                     AAGKPEEAEAWLRSVDLVYDGEMEAPKGKTPDDWEPVALPLFRSTKFGDDQDRPIKKS
                     DGNWTYFGADLAYHYQKAATADALVDIWGADHAGTVKRIKAAVAAMTGRDGAPKPFEV
                     KLVQMVALLKGGAPFKMSKRAGNFVTLADVVEMVGKDVVRFTMLTRKPEAQMDFDFDK
                     VVEASKENPVFYVQYAHARISSTLRKGAAEGFAPAAAALELLGTEELELVKLAAQFPR
                     LVEAAAQAREPHRIAFFLQDLAAAFHAYWNLGNDRPDKRFILGDNPALTGARLFLGAQ
                     IGQIIRNGLAVLGVAAVEEM"
     gene            37851..38834
                     /locus_tag="VFF98_13195"
     CDS             37851..38834
                     /locus_tag="VFF98_13195"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU64631.1"
                     /translation="MNGGRWQTGEGYGQAPGAFAPSVAGQGLAPGSGGSDAGGAGWVP
                     YPAGGAFAGHPGADHAGGDAHGAPLAEDGHAAPYHDAWLEHGEDRHAASGQEELDLAG
                     SARLPWLENGEDYEDDEPALDTRRVIGAAVAGVALLAAVVGGVWWISHRHDAEAAQGD
                     GSLIAAPTAPYKVAPANPGGKTYDGTGDTSFAVSQGQVRQGQLADGGPAGVPQGAASP
                     QLQPQPSPSAAAGHAAEGHVAAQGADNAQAAWAGAVVQVGAYSQRAQAEAAWGRLSTL
                     NPPLKGLHHRVVEGQADIGTVYRLQALTGAGGGGALCEQLRGAGVACQVKH"
     gene            38935..39957
                     /gene="nagZ"
                     /locus_tag="VFF98_13200"
     CDS             38935..39957
                     /gene="nagZ"
                     /locus_tag="VFF98_13200"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZU64632.1"
                     /translation="MIPAIFGLSGPVLTANERAFFRDADPAGYILFGRNVEGRAQLRA
                     LTDDLRSLHGRDRLLISIDQEGGRVARMKPPVWPAWPAGAPFAALWEAAPASAIEAAR
                     LNARALGMDLAEAGISLDCWPCLDVRVPGAHDVIGDRALGEEPVRVAALGRAVLDGLA
                     AAGVVGVVKHMPGHGRAGADSHKELPTVAASPADLAQDLAPFRALAQKALVGMTGHIL
                     FPAWDAVHPATQSARIIGEIIRGEIGFDGLLLSDDIDMQALSGTIPERAAAAIAAGCD
                     LVLNCWAKMDDMQGIAEACPTMPAGAPERLARALAPGAVPAARDAALQAELCAARDAL
                     LALAPA"
     gene            39954..40736
                     /locus_tag="VFF98_13205"
     CDS             39954..40736
                     /locus_tag="VFF98_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZU64633.1"
                     /translation="MNAPAAPQPERGEALYLELEGWEGPLDLLLDLARRQKVDLRSIS
                     ILELVDQYVGYIARAEALKLEVAADYLVMAAWLAYLKSAMLLPRDEQEDPSPEELALR
                     LQLRLARLGAMREAAARLMARDRLGRDVFARGAPEGLRADKRALWQCSWFDLVQAYGR
                     VKARTMPVVHMVRERPVMTLEAALARVSAMLEVELDWTELSAFLPAGEGAWADLRLRR
                     SALASSFVAALELAKQGRAEIWQEVPFGPLRLRPLAPTSAVP"
     gene            40750..41328
                     /gene="scpB"
                     /locus_tag="VFF98_13210"
     CDS             40750..41328
                     /gene="scpB"
                     /locus_tag="VFF98_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZU64634.1"
                     /translation="MSEDITRAVEAVLFAAPQPMSLAEIATHLGGGDVRAALARLSAD
                     YRGRGVELVERGGRWHFQTAPDLAHLLRREHEEPRRLSRAATECLAIIAYHEPVSRAE
                     IEAIRGVQTARGTLDVLMEAGWVRAAGRREVPGRPVIYATTPAFLAHFGLASRKDLPG
                     IEELRAAGLLDPVEEALEEALAGRPESPAPGA"
     gene            41474..41740
                     /gene="tatA"
                     /locus_tag="VFF98_13215"
     CDS             41474..41740
                     /gene="tatA"
                     /locus_tag="VFF98_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZU64635.1"
                     /translation="MGLSLPHLIVLAVVVLVLFGRGRISEMMGDFGKGIKSFKEGMAD
                     ETTNRPVTPPPAQIAAQNAPAPGVAPQAAATPATPAEPGQPGQG"
     gene            41766..42143
                     /gene="tatB"
                     /locus_tag="VFF98_13220"
     CDS             41766..42143
                     /gene="tatB"
                     /locus_tag="VFF98_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZU64636.1"
                     /translation="MLDIAPSEMLLTLAVAVVVIGPKDMPRALRTAGRWIGKMRRVSG
                     HFRAGLENMIREAEMEEMERKWREQNAAIMAAHPPVAEPAALTGPAPDAALAGPASTP
                     TPAPISTPAPAHGGDAPPPHAPA"
     gene            42190..43002
                     /gene="tatC"
                     /locus_tag="VFF98_13225"
     CDS             42190..43002
                     /gene="tatC"
                     /locus_tag="VFF98_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZU64637.1"
                     /translation="MAFEIKDIDDTQAPLLEHLMELRARLIRCLVALGLGFLVCFAFV
                     KQIFAFLVRPLAAAFPDGQGRLVYTKLYEAFFVELKVALFAGLLLAFPVIANQLWAFI
                     APGLYAREKRAFLPFLIATPVLFLSGAALAYYVVMPNAFRWLLGFGGSLGGLKLEALP
                     STGDYLDLVMQFIFAFGVSFLLPVLLMLLNRAGIVSRAQLKAARRYAIVGIVAAAAVL
                     TPPDVASQLMLAVPLLILFEGTLVVMWFTERRAAARASAGPEEPAEPEMPLP"
     gene            complement(43006..>43396)
                     /locus_tag="VFF98_13230"
     CDS             complement(43006..>43396)
                     /locus_tag="VFF98_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64638.1"
                     /translation="PAALPAPRARRLAAPAPPALPPLLRADVEKAIADLGETYTGDRR
                     WRIEEAEPVLEPAAEAAPPGEEAARAQAAANAEAARRAFAESRLGRIATGLYADFILT
                     GGDGAGAGGWQVWVSGRQVYAAERPAA"
BASE COUNT         6637 a        14220 c        15504 g         7035 t
ORIGIN      
        1 caggcaggca tcggccagcc aggccatgcg ggcatgcaag gtggcggcat cgcgcccctc
       61 gggcatcagg aaaacgcggg cgggggggat ggcatgggcg gccaccagcg catccacctc
      121 ggcgatgtcg tcgctcgtgg cgatcacata tttgaagaag gcgcgcgggt ctgcggcata
      181 ggcggccagg cgggcgggca gcagcgccag ctcggccggg ttgccgctgt gcgccagctt
      241 gggcgagacg ttgaactggt ggatcagcgg atcgagcgcg ggggccgcgg ccaccgtgcc
      301 attggtttcc acttccacat gccagccggg cagctctgcc agcatgcgcg ccagcgccgg
      361 accctgcagc agcggctcgc cgccggtgat gacgaggcgc ggctctggcc aatggccgcc
      421 cagcgcgcgg atgcgctggg ccacctcttc ctcctccagc atcagctgat tgtcggcgcg
      481 ggagaaatgg gcgtcatccg tgtggggccg gttgtcgccc tcgaagcgcc aggtataggc
      541 ggtgtcgcac cagcggcagg ccaggttgca gcgcgacagg cgcacaaaga ccgaggggcg
      601 cccgatggac gccccctcgc cctgcagggc ggcaaagagt tccggctcgc ccggccggat
      661 ggttgccagc gccagcatct taccccagcc tggcaagggc ggcggccagc cgttcggcct
      721 cggcggcatg gtgggcatga tcggcccgcg ccttgtcgat cgcctcgggc ttggcctttt
      781 cgacaaaggc ggggttgccc aggcgcttgt cgaggctgtc gcgctccttc tggctggcgg
      841 ccagcgcctt ggccaggcgc gccttttccg ccgccacatc gatcagccct tccagcggca
      901 cgatgagatt ggcatcgccc gcgcccacct gcatggcagc gcccgccggc gccggggcga
      961 aatggatggc cgaaaggcgc gccagccggt cgatcgcggc cgggttgcgg gcgatgatcg
     1021 cgcgggtggc ctcgctcacc tcgggcagcc aggcttccag gcgggcgccg ggggccagcc
     1081 ccagctccac cttggcgccg cgcagattgc cgatcagcgc gatcagccat tccacctcgg
     1141 ccttggcctc ggcatccacg ctggcttcgg gcgcgggcca gcgcgccacg atcagctcgt
     1201 tggcgcgcgg ggcatcgggg ctggccagcg ccgaccacag ctcttctgtg acaaacggca
     1261 tgaaggggtg cagcatgacg aggatctgat cgagcaccca gccggcaacc gccctggttt
     1321 ctgcatcgaa cgagcccttg atcagctcga tataccagtc gcagaagcca tcatagacga
     1381 agcgatagat cgcatgggca gcggcatcga agcgcagctc ggccatcgcc cggtcgagcg
     1441 cggcgagggt ttccaccacc tcgccgatga tccacttgtt gaccgcgctg gtggccacag
     1501 gggcggccaa ggaagccgag ccgacaatcc cgttcgactg gcagaagcgc gccgcattcc
     1561 acagcttggt ggcgaagttg cgatacccct ccacgcgctt ttcatccatc ttcacatcgc
     1621 ggccctggct ttccatcgcc gccatgaaga agcgcagcgc gtcagcacca tactggtcga
     1681 tcaggcccag cggatcgacg acattgccct tggacttgga catcttctgc ccatcggccg
     1741 cgcgcaccaa cccgtggaga taaagccgct tccacggcac gcccggcagg aaatgcatgc
     1801 cctgcatggc catgcgcgca tcccagaaga acaggatatc aaagcccgag acgagcagat
     1861 cgttgggata gtgcttctgg aacagcggcg cgttttcatc cggccagccc agcgtggcaa
     1921 agggccacag ggccgaggag aaccaggtgt ccagcacatc gggatcgcgg gtgagggcct
     1981 tgttgccggc caggccttgc gcctcttcct cggtttcggc cacgaacacg ctgccatcct
     2041 cggcatacca cgccgggatg cggtggcccc accacagctg gcggctcacg caccagggct
     2101 ggatgttctc catccagttg aagaaggttt tctcccaggc cttgggcacg atctcgatgg
     2161 cgccgctctt caccgcttcc agcggctttt gcgccagcac gccggcattc acataccact
     2221 ggtcggtcag ccacggttcg atcaccacgc cgccgcggtc gccaaagggg gtctggatgg
     2281 tgcgcggctc ggcgtcgtgc tcggtctcgt tgccctcctt gtccttggtg acatggggga
     2341 tgaggaagcc cagttccttc atcttcgcca cgatcatgcg gcgcgcctca aaccgctcca
     2401 cacccagata ttcggccggg atcaggccat cggcggtctg caccacgttg gcatcggcat
     2461 cgaacatgtt gagcatgtcg ttgggggcaa tgccggcgcg cttgcccacc tcaaagtcgt
     2521 tgaagtcatg gcccggggtg atcttcacag cgccgctgcc cagctcgggg tcggcgtgtt
     2581 cgtcggccac gattttgaaa cggcggccgg tgatcggctg gaggatctcc ttgccgatca
     2641 cgctctggta gcgctcatcc tcggggttca ccgccacgca catgtcggcc agcatggttt
     2701 cggggcgggt ggtggccacc tcgatgtaat ccagcccgtc gtcacgcaga acgccatcgg
     2761 ccagcggata tttgaagtgc cagaagccgc ccttcacctc gcgcgtttcc acctcgaggt
     2821 cggaaatcgc ggtcttcagc ttggggtccc agttcaccag ccgcttgtcg cgatagatga
     2881 ggtcctggtt gtaaagatcg acgaacacct tcaccaccgc gcgggtgaaa tgcgggtcca
     2941 tggtgaactg ctcgcggctc cagtccatcg agcagcccag gcggcgcagc tggcgggtga
     3001 tggtgccgcc gctttccgcc ttccattccc acaccttgtc gatgaagcct tcgcgggtgt
     3061 aattggtgcg cttgtcgccc ctggcctcca tctggcgttc caccaccatc tgcgtggcga
     3121 tgccggcatg atcggtgccc accacccaca gcgcatcctt gccgcgcagg cgctcatagc
     3181 ggatgacgat atcctgcagc gtgttatcca gcgcatggcc gatgtgcagc gagccggtga
     3241 cattgggcgg cgggttgacg atggtgaagg gcgcggcatc gggccgttcc gggcggaaca
     3301 ggccaccttc ttcccaatgg gcataccatt tggcctccag cccgctgggg tcgaaggtct
     3361 tgtcgagagt gggctgcgcg aaatcctcgg tcatgcccgg gcctttgcca gcccggccgc
     3421 atgcgtgcaa gggggccgcg cggggagggg gcgtgggcgg cggcgggccg gcttttcctt
     3481 gcccttgccg gcattttccc gcattacccc tcgccagatg gaccagctgc ctgattttac
     3541 catcaaaacc gatgactcgg gcgctgtgga actggcgctg gcggggccct tggtggtggc
     3601 gcgcatcgcc gcgctcgaca aggccctgcg cgcgctggcc ggccctgtgg cgcggctgga
     3661 tctggccggc gtcaccgcga tcgacacggt gggggcctgg ctgtgctggc gcacggccga
     3721 tgcgctgggc gccgagatca cccatgccga cgaggattcg cgccgcctga tcaacgccgt
     3781 gcgcggcgat caggaggagc tgccgcctcc gcctccgccc cagcccgcct ggcgcgagac
     3841 gctggcaagg ctgggccagc tggtggtgga tggcgccact ggcgctgtcg atgtgctggc
     3901 cttcatgggc gcgctgctgg aggcggcctg gggcattgcg cgccatcccg gccggctacg
     3961 cggcacggcg ctggcccgcc agctgcagct ggtggggatt gatgcgctgg gcatcgtcgg
     4021 gctgatgagc tttctcatcg gcatcgtcat cgcccagcag ggcgcggtgc agctgcagca
     4081 gtttggcgcg cagatctaca cgatcaacct caccggccgc ctcgccttcc gcgagctggg
     4141 ctgtttgatg acggcgatca tggtggcggg ccgctcgggc tcgtcctttg ccgcgcagct
     4201 gggcacgatg aagctgaccg aggagatcga tgccatgcgc accatcggcg tctcgccgct
     4261 ggaagcgctg gtgctgccgc gggtgctggc ggcggtgtgg atgctgccgc tgctgggctt
     4321 ctattcggcg ctgatcgcca ttgtcggcgg cgcctcgatc tcggcggtgg tgctggatat
     4381 tcccttcgtc accttcctgc tgcgcattca ggaggtggtg ccgatccgcg acttgtgggt
     4441 gggcctgatc aaggcgccgg tgttcggctt catcatcgcc atcaccggct gctatcaggg
     4501 cctgcgcgtc tccggcaatt cggaggaagt gggcctgcgc accaccatgg cggtggtgca
     4561 ggcgatcttc atggtgatcg tgctggatgc gtttttcgcg gtgttcttct ccggggtggg
     4621 ctggggatga acgaggcgct ggcaaccgcg ggcgattgcc cgatccggat cgaggggctg
     4681 gaaaaccgct ttggcgagca tgtcgtccac gagaacctct cgctcagcgt gcggcggggt
     4741 gagattctgg gcgtggtggg tggatcgggc acaggcaaat cggtgctgat gcgctcgatc
     4801 atcggcttgc agcatccttc ggccgggcag atctcggtgc tggggcatga tatctgccgg
     4861 gcagatccgg ccggcaatgc cgatctgcgc tcgcgctggg gggtgctgtt ccagggcggc
     4921 gcgctgtttt ccacgctgac ggtggcggaa aacgtggaag tgccgctgaa ggaattctac
     4981 cccgacattg accctgccct gcgccgcgag atcgcccgct tcaaggtgcg cctctcgggc
     5041 ctgccggcag aagcggccga caaatacccg tccgaactgt cgggcgggat gaagaagcgg
     5101 gcggggctgg cccgcgccct ggcgctggac ccggagctcc tgttcctcga cgagccgacc
     5161 gccgggctcg acccgatcgg cgctgccgct ttcgacaagc tgatccgcgc gctgcaggca
     5221 acgctgggcc tcaccgtctt cctcatcact cacgatctcg acagcctgca cgccatctgc
     5281 gaccgggtgg cggtgctggc cgacaagcgg gtgatcgcca ctggcaccat ccccgagctg
     5341 atggaaaccc atcatccctg gatcgaggaa tatttcaacg ggccgcgcgg gcgctcggcc
     5401 ctgctcagcc gcgctggcga tgcagcgccg ccaaccgcca cggccgcgct ggacaggccc
     5461 ggcaaaccaa aggcatagtg agacagatgg aaacgcgggc caatcatgtc tgggtgggcg
     5521 cggtcacgct ggcgctgctg gcgctgatcg cggcggcggc catatggatc gcgcgcgtgt
     5581 ccgaaggggt gaagaacgat tacgacatct tcttccgcca gtcggtcgag gggttggggc
     5641 gcggcagcgc ggtcacctat gcgggcgtgc cggcaggcaa ggtggtggag atcgagctgt
     5701 ggaagaagga ccccagcctg gtgcgcgtgc gcatcgcggt ggatgatcgc attcccatcc
     5761 tggccggcac caccgccagc ctgcagggca gcttcacggg cgtgtccact atccagctca
     5821 acgatggcgt gaagggcgcg ccgccgatca ccgcgccagg gcccgaaggc gtgcccgaga
     5881 tccccaccaa gcggtctggc ctgggcgcgc tgctgtcgaa cgcgccggtg ctgatggacc
     5941 ggctgaccag cctgaccgag cggctgggcc aggtgctgtc ggatcagaac atcgcccata
     6001 tcgacggcgt tttcgccaat accgacaaga tgaccagggg cctggccgag gccacgccgc
     6061 agctgcgcac ggaccttgcc gatctggacg ccaccctgca gcaggccacg caaacgctga
     6121 cggccttcca gaaggtggcc gacagcgcca atgcccagct cgaccccaac ggtccctcgc
     6181 tggtgcacca gatgcgccag acgctgaaat ccgcccaggc cgccgccgat gccctgcatg
     6241 gcgagctgga cgaggcgcgc ccggccaccc accagctggc caccagcacc ctgccgcaaa
     6301 ccgaggctac cttgcgcgac ctgcgcgaga ccagccgggc cttgcgcgaa attaccgaga
     6361 agatcgacaa tcagggcgcc agcggcctgc tgggcgcgcc caagctgccc gagtacaagc
     6421 catgatccgc gctgcttcct ttcgttgcct gctgcctgcg ggcgtgtttt tcctgggcct
     6481 ttcgggctgt gtgagcctgg ggggcggcaa ggcgccggaa cgcctgttca ccctgcgcag
     6541 cgatgccgtg gcggccagcg ggcaaacggt ttcggccagc agcgccgagg cggtgctggt
     6601 ggtggagccc gataccgacc gcagcctggc cctgcagcgg gtgccggtgc aggtggatgc
     6661 caatgccatt gcctatctca aggatgccaa ttgggtggag cggcctgcgc gcctgttccg
     6721 cacgctgctg gcagaacata tccgcgccgg cggcaagcgg ctggtgctgg aggaggatgg
     6781 cgcgctgccg gccggcgcca tgcggctggg cggccggttg acggcaatgg gctatgacgc
     6841 gcgcagccac agcgtggtgg tgcgctatga cgcgctgcgc gacgagggtg gcggcaagct
     6901 ttccgcccgc cggttcgagg cggtggaggc ggatgtgccg gcccaggccc atgcggttgg
     6961 cccggcgctg aaccgggccg ccaacaaggt ggccagcgac gtggccgcct ggatcggctg
     7021 aggtttaccc gcgcgccagc gcagcgaagc gggcggcgcg ggcgaaatcg gccgtgcggc
     7081 gcgcccggcg ggcttccgcc tcggcatcac ggccccacag gtctgcctgc cattcctctt
     7141 ccaggcaggc cgcctgccac agggcggcca catcggcccc cggctccagc gcggccagcc
     7201 ccaccaccag cgaggcggct aggctgacga gattgcgcag cgccgccagg gcaaaatcgc
     7261 cttcggccgc cagcgccgcc tgcaggcggg ccaaggtggc ggccggctgg gggcggtgga
     7321 tgatgccgct cacccgctcg aaatggatgt cgtagcgcgc ttcggtggcg ttcagcagcg
     7381 gttcccacac ggcgatctgg cgatcgagca gggcgctgcc ttcatccgcc cggtagcaca
     7441 gcgtgtccgt ctcgccatag gggatcagcg cggcctgcgc ggcggcgcgg tcgggcgcga
     7501 tcacgtcgag cgcataatcg gccatgtcgc gcaagggcag ggcggcggca tcgatcttct
     7561 cgccctgcgc ggcccattca tcggccagcg ctgcggccag cgcctgggtg ggggcaatct
     7621 gcggggcgcc gcccacggtt ttgatcggcc ggccatcgag cagcacgcgc cagccgccat
     7681 cggccgcggc cgtgctcaca tccttgtaga aacgcttcat cattctgcct gttcgttgaa
     7741 gggggatcag ccggcgcgca gcatctcgcc cagttcgcgc acgctgttgg ccacgcccac
     7801 agcgccggcc gccagcaatt cggccggcgg gtgatagccc caggcaacgc cgatggcgcg
     7861 cacgccggcg gcctcggcca tggcgatgtc gaaagcggtg tcgccgatca tcaccgcctg
     7921 atgcggctgg gccaggcatt cggccagcgc ggcttccagc atggcggggt ggggcttgga
     7981 agggtggcgg tcggctgtct gcaggctgtc gaaatggctt tccagcccgt gggcggccag
     8041 gcaatgagcc aggcccctgt cgctcttgcc ggtggcgatg gccagttgcc agccttccgc
     8101 ccgcagttca tccaccagcg cgcggatgcc ggggaacagc ggctctgcca gccggccttc
     8161 ctgccgggcg cggcggaagg cggccttgta ggcctcgacc agatcggcca ccagctgggc
     8221 atcggcctcg ggcgccagcc gcgccatggc ggccggcagg ctgaggccga caatgcgccg
     8281 caccgcttgt gccgggcgtg ccggcaggtc gaggcgggca aaggcctcgt ccatcgccgc
     8341 gatgatatcc gcctggccat cgaccagcgt gccatcgcaa tcaaaaacgg cgaggcgcga
     8401 ggacgccatc agcgcttgcc ccggggggcg ccgccgggcc gggccgggcg ggccgcgccc
     8461 ttgccgggat ggccggcatg gccgccgggc ctgccgctgc ggctgctgtc agggcgcgcg
     8521 ctggccggct tgccgccgct tttgggcgcg cccgggcgcg ggccgccggg cttggggctg
     8581 ccgggcttgc gggggccctt gcgcgctgcc gggccggcat tctgcccact gccctgatgg
     8641 cccccaccat gatgcccacc accgtgcgtg gcatcgttgc ggctgcggcc ggtgtcggcg
     8701 cggccgcgcc gctcgccccg gcgttccttg cgatattgct tggcatgggc gcgcgcggcg
     8761 ctcttctgct cctcgcggct gaaatcgccg gagggatcgg ggatatattc gccctcgctc
     8821 tcctcgaagc ccaactgggc gagcgaggcg gcgaagtgat cgggcagggc ggcggtcacg
     8881 tcgagcgggg tgccatcggg gtgctcgatg atcaggcggc gggcgtgcaa gtgcagcttg
     8941 cggctgatgc tgcccgacag gaacgcctcg ggcccgccat atttgccatc gccgatgatc
     9001 gggtggccaa tggcggccat atgcacgcgc agctggtggg tgcgcccggt cagcggttgc
     9061 agctccaccc agcaggcgcg gttgccggcg cggtcgatca cccggtagcg gcttttggaa
     9121 ggctggccgt gctcgctctg gtcgaccatc atcttctcgc cgccgctgcc cggctgcttg
     9181 gccagcggca gatcgatcag cccttcatcg atgctgggca cgccggccac cagcgcccag
     9241 tagatcttgc gggcttggcg cccggaaaag cgcttggaaa agaaggccgc gctgcccggc
     9301 gttgccgcca ccagcagcac gcccgaggta tccttgtcga gccggtggac gaggcgcggg
     9361 cgggtgctgc cggtggcata ggcatcgagc aggccatcga catgttcata ggtgccgctg
     9421 ccgccttgcg tggcaaggcc gggcaccttg ttcagcacga tggccgccgg atcgcggtgg
     9481 atcaccatgc tgtcggccag ctcgatctgc gcctcggtca gcggcttgcg ggcgcgcggg
     9541 gccttgccgg cgccgcctgg cgcgtgggaa tcgcccggcg gcacgcgcag ggtctggccc
     9601 gccaccagcc ggtcttccgg cgcggcgcgc ttgccatcca cgcggatctg gccggtgcgg
     9661 gcccagcggc tgatggtggc aaagcccacc tggggcaggt ggcgcttgaa ccagcggtcg
     9721 agccggatgc catcatcgtc gcggccgacg acgaactggc gcactttgtg ttctgtctca
     9781 ttcatcggcc cctgttagcc gagcatgggc gcgctgaaca ggccggcatt tcgcctccct
     9841 tttgccaata cggcgaactg gcccgccggg catgggcggt ttggcccttg caaggggcgc
     9901 aggggcaatg gcatcgcccg gccatccgat gttccgatgc atccgatgca agggggaggg
     9961 caggttgcgt tcgatccaca caccgaaaat cgatgccgct tattggctgg cgattgtgct
    10021 ggccagcatt gccggcacca atctgggcga tctttacgcc gatcgtagcg ggctgggcat
    10081 atttggcggg ctgtggctgc tggtggcgct ggccgcggcg gtgttcgtgg ccgagcggcg
    10141 cgatcgccgc gcgcatgagc tgtggttctg gctggtgatc ctgatcatcc gcaccggcgc
    10201 caccaacatc gccgatggca cggcgcatct gtggctggcg cggccggacc tgtcggcgct
    10261 gacggcggtg ttgctggcgg tggcggccgg tttttcgcgc tgggcagagc cggggcgcag
    10321 cctgaaggcg gatgcgccct tctggctggc gatgctggcg gccggcgtgt ttggcacagc
    10381 ggccggcgat gtggcgcagc acctgctggg cgattggcag gcctgctgcg tgctgggcgc
    10441 ggcgctggcg ggcgcggtgg cgctgtgggt gcgggtgcgc agcctggcgg cctattggct
    10501 ggcggtggcg ctggcgcgca cggcgggcac ggccattggc gatgtgctgg ccgagggcgc
    10561 ggtggatatc ggcatcatcg ccagcacggc gctgtcgctg ccgttattcg cgctggtggc
    10621 gggctggggg cagcgccggc gggccctggc ggcggccgcc tgagcccggt gttccgcctt
    10681 gcaaagcctt gcctttgcgg cgcgactctg ctagcggcgc ccaatcgtgc cggcccccgt
    10741 gggattcggt gggtttcgta tttcttcaat ccagggtgtt cccccgcacc aggcctttaa
    10801 ccggccatgc cgcggggttc tcgccggcta gtctttcgag gtggttcttt tatgcaaatt
    10861 ctcgtccgcg ataacaatgt cgaccaggct ctgcgcgcgc tcaagaagaa gctgcagcgc
    10921 gagggggtgt atcgcgagat gaagctgcgc cgccactacg agaagccctc ggaaaagcgc
    10981 gcccgcgaaa aggctgccgc cgtgcgtcgc gcccgcaagc tggagcgcaa gcgcgccgag
    11041 cgtgacggcg ccaagtaagg cgctggtggg cctggcgcca ggcggcgggc cccacctgca
    11101 ataaagacag ccatcgggct tgccccgcca gcgctgttag gccatgaggg cgcggaatca
    11161 ttcccgcgcc ctttttggta tgccggggct ggccgcagcg ccggcggttc tgtttctgtt
    11221 gtcttttttc aaggacgagc acgatgaccg agatcacccg cgttcccctc cagccgctga
    11281 ccaagggctc catcaagaag ctgtggctgg gcgttgttgc tgccagcctg gcgggtgcgg
    11341 cgattgccgg tgcgggcatg ccgccgctgg tgtccgtcaa gacgctgacc cccggcttcg
    11401 gccccagccc ctcgggcgat gcgctggtgc agatcaacta tgtcggcaag ctggccaatg
    11461 gcaccgtgtt cgatcagggc aagggcgccg tgctgccgct gcagggcacc attcccggct
    11521 ttgccaaggc gctggcgcaa acccagaagg gcggcaagta tgtcttccgc attccggcgc
    11581 gcctggccta tggcccgatc gcccagggcc ccattcccgc caattccgac ctcaccttcg
    11641 aggtggaagt gctcgatttc cgcagccgcg ccgaactgga agcgcagcag cgcatgatgg
    11701 agcagatgat gcgcgcccag ggtgcccatc agcaggcgcc ccaggctggc gatgccgcgc
    11761 cggcgccgca ggctgccccc gcgcaataag caccagcctg gccgggcggc cccgccgccc
    11821 cggccagccg gccccagcac ggcgggcgcc ctttaggggg tggcccgctt gcggccgtgg
    11881 cggcgcggtg ctagcgcagc accggcaagg ggcggcccca aggggtggcc cggtgccgcc
    11941 cgcatctgca caagatccca tccgcacaag atcagctgaa ggttttcgta tgtcggtaac
    12001 gaccgaggaa gtggcccgga tcgccgcgct cgcccgtctg aaggtgacgc aagaggagct
    12061 ggccgccatg gtgcccgagc tcaatggcat tctcgcctgg gtggaacagc tgggcgaagt
    12121 ggacgtgacc ggcatcgcgc cgatgaccgc tgtcatcccc aaccatctgc gcctgcgcgc
    12181 ggatgtggtg aacgccgacc cgctgaccgg cggcggcatc cgcgatgccg tgctggccaa
    12241 tgcgccggcg gccgaacatg gcttcttcgg cgtgcccaag gtgatcgaat aatgaccgag
    12301 ttgaccgaac tgacgctggc cggcatccgc gatggcgtgg ccgccggcac tttctccgcc
    12361 actgaagtgg ccgaggcttt caacaccgcc gtggccgatg cgcaggaagc gctgaacgcc
    12421 ttcatcgtca ccacgcccga agcggcgctg gaggcggcca agaaggtgga cgccgaccgc
    12481 gccgccggcc gcccgctggg caagctggcc ggcgtgccga tcggcatgaa ggacctgttt
    12541 gccaccaaag gcgtgcagac caccgctgcc agccacatgc tggaaggctt catcccgccc
    12601 tatgaaagca ccgtctcgca gaagctgtgg gatgcgggcg cgggcatgct gggcaagctg
    12661 aacctcgacc agttcgccat gggctcgtcc aacgagacca gccatttcgg caatgtcatc
    12721 agcccctggc gccgcccggg cgagaccacg ccgctggcgc cgggcggctc ttcgggcggc
    12781 tcgtcggcag cgattgccgc gcggctgtgc ccggccgcca ccggcaccga caccggcggc
    12841 tcgatccgcc agccggccgc cttcaccggc atttccggca tcaagcccac ctatggccgc
    12901 tgctcgcgct ggggcatcgt ggcctttgcc tccagcctcg atcaggcagg ccccatggcg
    12961 cgcaccgtca gggactgcgc gatcatgctg gaagccatgg ccggcttcga ccccaaggat
    13021 gccaccagcc ttgatctgcc ggtgcccgcc tgggaagccg ggctgaaccc ggacctgcgc
    13081 ggcaagaagg tgggcattcc gcgcgaatat cgcatcgagg ggctgaacgc cgaagtggcc
    13141 aagagctggg acgatggcat cgcctggctg aaggatgccg gcgccgagat cgtcgagatt
    13201 tcgctgccgc acaccaaatt tgccctgccg acctattaca tcatcgcgcc ggcggaggct
    13261 tcctccaacc tcgcgcggta tgatggcgtg cgttatggcc tgcgtgatct gcccgagggg
    13321 gcaaacctgc aggagatgta tgcggccacc cgcgcggccg gctttggcgg cgaggtgaag
    13381 cggcgcatcc tgatcggcac ctatgtgctt tcggccggct tctacgatgc ctattacacc
    13441 caggcgcaga aggtgcgcgc gctgatcgcg caggatttcc tgaaggcctt cgaggaggtg
    13501 gatgtgatcc tcacgcccac ggccccgtcc acgccgtttg ccctgggcga aaagcgcgcg
    13561 gatcctgtgg aaatgtatct gaacgacgtg ttctcggtgc ccgcttcgct ggcgggcctg
    13621 ccggcgatga gcgtgccggc cggccttgcc agcgatggcc tgccgctggg cctgcacctg
    13681 atcggcaagc cgtttgacga ggccggcgtg ctgaatgccg gccttgccat tgaggcccgc
    13741 gccggtttcc acgccagccc cgcaaagtgg tggtgaatat gagcaagtat cgcattcagg
    13801 gcgcaacggg cgaatgggag gtcgtgatcg gccttgaagt ccatgcgcag gtcacgtcgg
    13861 gttccaagct gttttcgggc gcggccaccg ctttcggggc agagccgaac agccaggtct
    13921 cccttgtcga tgcggcgatg cccggcatgc tgccggtgcc caaccgcgag tgcatccgcc
    13981 aggcggtgcg caccggcatg gcgatcgagg ccgagatcaa caaatggtcg cgcttcgatc
    14041 gcaagaacta cttctatgcc gatctgccgc agggctatca gatcagccag ctctatcacc
    14101 cgctggtggg cgaggggcag ctgaccatcg aggccgacga gaaggccggc ctgcccgagg
    14161 ccaagcagat cggcatcgag cgcatccatg tcgagcagga tgccggcaag ctgatgcatg
    14221 accagcaccc gacgatgtcc tatgtcgatc tcaaccgctc gggcgtggcg ctgatggaga
    14281 tcgtcagccg gccggacatg cgctcgccgg ccgaggcggg ggcgtatctg gccaagctgc
    14341 ggcagatcct gcgctatgtg ggcagctgcg acggcaatat ggaccagggc agcatgcgcg
    14401 ccgacgtgaa cgtgtctgtg cgccgcccgg gcgagccgtt tggcacgcgc accgagacga
    14461 agaatgtgaa ctcggtgcgt ttcgtgatgg ccgtggtcga atcggaggcg caccgccagg
    14521 tggcgctgat cgaggatggc ggcacggttg ctcaggaaac ccgcctttac gatccggacc
    14581 ggaacgagac ccgcagcatg cgcagcaagg aagatgcgca tgactatcgc tatttccccg
    14641 acccggacct gctgccgctg gagctggacg atgccttcct tgaggaatgc cgcgccagcc
    14701 tgcccgaact gcccgatgcc aagcgcgccc gctatgtgga tgggctgggc ctgtcggcct
    14761 acaacgccca tgtgctgacc gccgaggtcg acaccgcccg ctggttcgag gcgctgctgg
    14821 cggccaccgc cagggcgcag ggcaagggcg aggcggaagt ggccaagcag gcctccaact
    14881 ggctgacgag cgagttcttc ggcgcgctca acaagctggg caaggatctg gccaccagcc
    14941 cggtatcgcc cgagggcgcg gccgaactgc tggcgctggt gggcgatggc acgatttccg
    15001 gctcgatcgc caagcaggtg ctggaaaaga tgctggagag cggcgagggc gccggcgcca
    15061 tcgtcgagcg cgaggggctg aagcagacca gcgataccgg cgccatcgag gcggtgatcg
    15121 ccgatgtgct ggcgaagaac accgacaagg tggaccagta tcgcggcggc aaggaagccc
    15181 tgttcggctt cttcgttggc cagacgatga aggcgatggg cggcaaggcc aatccgcagc
    15241 tcgtcaatga attgctgaag aaggcgctgg gctaggcgcc ttcacagggc ggcaagcctg
    15301 gttctggcaa agaacaggct tgcactctgc cgatagctgg taaatcctcg taggtatgag
    15361 gattaccggg ggcggtatgg caatctggcg cggcgggatg gggatgctgg cggcaacagc
    15421 gctgctgggc gcaggcgtgc cggccatggc cagccgcggc gcgccagctg ccagtcgcag
    15481 cgcggccccc accagcgaca cggccgagga cgaggacgcc gataccgaca cctctgcccc
    15541 gatgagcggc gaggatctca ccaatgcgca ggtggtggcg ctcaaccgca cgcggctggg
    15601 cgatccggcg ctggcgcaga tggtgcgcgt gtgcaaaccc cattttgata tatcgacgcg
    15661 ggcgctgctg gccttgcggg cggcgggcgt gtccagccgg gtgatcgcgg cgatgatcaa
    15721 ggtgcaggcc aaggccgatg gcacgcgcga ggacaatgat tcgcccgatc cgcatcagcc
    15781 ccatggcgcc ggcctctatc tgctggccgg ctggctggag gcgccgcgca tgttcgccct
    15841 tgcgccgcag acgaccaccc gcaccacttc gggcagcata ttgggctatg ccttttccgg
    15901 cggcatcgtc tctgtcggct acaaggcgat gctgcccggt gcccatgcgc cggtcatcgc
    15961 cgaaggggcg cggccggtgt tctatgtgtt tggcgccaag gcgggcaatg cctgggggcc
    16021 ggtgcccgcg cccgaggaag tgtcgctggt gcgcttcagc cagggggcgc atgggcgcga
    16081 agtgcgcatc ggcagcttca acatccatgg cgccagcacc ggcatccgcg atgccgaccg
    16141 tattgccttc cgcatccgcc agctggggca gggggcgatc gagctgcgcc cggaggagga
    16201 tctggcgccc ggcgaatatg gctttgtgca gacggcggcg ggcgtgggca ttggcgcggc
    16261 gcgggccagc accagcagcg cgcgggtgtt tgcctttgcc gtgggccccc atggcagcca
    16321 gccggcgcaa ttgccccagc cgggcaccag cgtgcagcgc ggccccggga tggaccggct
    16381 gaacgtgcgc gccggccggc gcaccgccgc tgtcagcggc atggagggcg gggcgggtaa
    16441 cacgggccag caaagcaatg ccagccagcc gggcaataca ggccagccag ccaatgcgcg
    16501 caaatccggc cagccggcca gcggggccga atggggcgac agcggctggg cctattgagc
    16561 ggggcttccg ttcatctcct gttgggcctg gccggctaac ggattagaca gagccgctgc
    16621 ccgccaaggg gccagcccgg agtgaccatg ctgtattcca cgcgcctgtc gaagatcgcc
    16681 cgccccgcag cccgcttttc ctctgccagc ctgatcgccc tttgccttgc cggcaccagt
    16741 ctggccggaa ccgcgccatc tgcccccgcg gccccgccgg ccgccccgct ggccgatctg
    16801 gtggcgcagg tgaacatccc ctttgaacgc ttccagctgg ccaatggcct caccgtcatc
    16861 gtccacaccg atcgcaaggc gccgatcgtg ggggtgacga cctattaccg cgtcggctcg
    16921 aagaacgagc ccaagggcca caccggcttt gcccatctct atgaacatct gttcttcggc
    16981 ggcagcgcca atgcccccag cttcgatgaa ccgctggagc aggccggcgc caccgccacc
    17041 aacggctcca ccagctatga ccgcaccaat tacgtggaaa ccgtgccgaa gggcgcgctc
    17101 gatctggcgc tgttcctcga aagcgaccgg atgggccact tgctcgccgc cgtcacccag
    17161 gacaagctcg acaagcagcg cggcgtggtg gaaaacgaga agcggcagga tgacaaccag
    17221 ccctatggcc tgctctccta tgcgctggcc gaggggctgt ttccggtggg ccacccctat
    17281 cgccacacgc cgctgggatc gatggccgat ctggatgcgg ccacgctggg cgatgtgcgc
    17341 gcctggttca cccagcatta tgcgccgggc aatgtggtgc tggtgctgac cggcgatatc
    17401 gatgcggcca ccgcccgccc gctggtggag cgctatttcg gcgcgatccc ggccggcccg
    17461 aaagtggagc cggtggccgc agggccggtc acgctggccg cccccagggc gcgggcgatg
    17521 accgacaggg tggccaccac cacgctctat cgcgcgtgga gcgggccggg ggtgaacgac
    17581 cctgatgccg aggcgctgga gatcggcatg accgttctgg gcgggcttgc ctcctcgcgg
    17641 ctggacaatg cgctggtgcg cggcaggcag ctggccgtgg gcgtgaccgc cagcgacgag
    17701 cagaacgagc aggtctcgca gttgaccgcc agcctgtcga tcaagccggg cgtgccgcgg
    17761 gccgaggcag aagccgcctt cgatgccgag atcgcccgct tcctcgccga agggcccacg
    17821 gccgatgaat tgcgccgggc ggccacccgc acggtggccg aggagatcgg cgcgctggaa
    17881 gtgattggcg gctttggcgg caagggcgcg caactggccg aggggcagct ctattccggc
    17941 gatccggcgc attaccgggc ggaactggcg cgcattgcgg cgctcacgcc cgaggccgtg
    18001 cgcgatgcca tgcgccgctg gctgggccgg ccggcctata atctcaccgt caaccccggc
    18061 gagcgcaccg agaagggcga tgcgatgggc ggctggggcg atgaggcggc aacgcccgcg
    18121 cgcccggtgg atgccaagaa gcccgtgccg ccgatcgccg ccggggcgcc gcgcccgcag
    18181 ccggcggtgg ccccggtggg ggcgctggcc ttcccggcgg tggaacatgc gcgcctgtcg
    18241 aacggcatgg cggtggatct ggcgcggcgc acggcggtgc ccaaggtgct ggtcagcctt
    18301 ggctttgatg ccggcgtggc cgccgatgcg gtggatgcgc cgggcacgca ggggctgctg
    18361 ctggcgatgc tgcaggaagg caccgccggc gcccacccgc gcgatgccac gcaggtgctg
    18421 gaagagcagg agcggctggg cgccaccatc agcgccggcg ccagcctgga taccagcacc
    18481 gttacgctgg atgcgctgaa ggccaatctg gcgccttcgc tggggctgtt ggccgatgtg
    18541 ctgcgcagcc cggcctttgc cgcgggcgat ctggcccgag tgaagcagca gcgcctcacc
    18601 gcgctggagc agacgctggc cagcccccag ggccaggccg cgcgcgcgct caatccgctg
    18661 ctgttcgggc ccggcacccc ctatggccag ccgggcgacg ggctgggtag caaggcgagc
    18721 cttgccgcgc tggatgggcc tgcgctgcgc aaggcgatgg gcgaatggct gcggcccgac
    18781 aatgcccgca tcaccatcgt cggcgatatc accatggccg aggccctgcc gctgctggag
    18841 cgcgcctttg gcgattgggc gccgccggcc gctgcccgcc cggccaagcc cgatgtggcc
    18901 ccgccgccgc cggcgccgcg catcgtggtg atcgaccggc cgcacagccc gcaatcggtg
    18961 atcctgggcg ggcgggtgct gccgctgtcg ggccgcgatg cggtgatgga gccgctgggc
    19021 gtggccaatg acgtgctggg cggcgatttc ctcagccgcc tcaacaccga tctgcgcgag
    19081 gagaagggct ggagctatgg cgtttccacc accatccgcc agccgctggg gcgcgccagc
    19141 ctcatcgtct atgcccccgt gcagaccgac cgcaccggcg atgccatcaa ggcgctggtg
    19201 gccgacatga aggcgctgcc gggccggcag ccggtggccc cggcagagct ggcccgcgcc
    19261 accgatggca ccatccgcca gctgcccgcc cgcttcgaga cgaacgggca ggtgctgggc
    19321 gctgtcagca ccaaccagcg ctatggccgg ccggatgatt attacgtcac cctgccgcag
    19381 cgcctgcgcg gcgtggatgc caagcaggcc gatgcgctgg ccgcgcgcta tctccagccc
    19441 gatgggctgg tgtttgtggt ggtgggcgaa aaggcggcga tcgccccgca gctgaaggac
    19501 ctgggcctgc cggtggaata tgtttcgggc ggggcgggtg aataaaattc gcagcgaatt
    19561 tgcgctggct ccttgtgtgg ccaaatggtc acactatctg tggcagggaa gggagaaccc
    19621 cgcatgaacc tcgaaaaatt cactgaccgc gcgaagggct ttttgcaggc cgcccaggtg
    19681 gtggccatcc gcaacaacca ccagcgcatt acccctgagc acctgctgaa ggcgctgctg
    19741 gaagatgagg aaggcatggc tgccggcctc atccagcgcg cgggcggcac gccggccttt
    19801 gctgtgcagg aagtggacaa ggcgctggca aagcagccgg cggtcaccgg cagcggcgcg
    19861 cagaattccc ccggcctcga caatgaggcg gtgcgcgtgc tggatcaggc cgagcagctg
    19921 tccgccaagg ctggcgatgc cttcgtgccg gtgcagcggc tgctgcaggc gctggcgctg
    19981 gccaccacga cgcctgccgg gcaggcgctg aaggcggcca atgtcagccc gcaggcgctg
    20041 gagacggcca ttcaggagct gaccggcggc cgccatgccg attccgcctc ggccgagaac
    20101 gcctatgagg ggctgaagaa attcgcccgc gacctgaccg aggccgcgcg cgagggcaag
    20161 ctggatccgg tgatcggccg cgacgaggag atccgccgca cgatccagat cctggcccgg
    20221 cgcaccaaga acaacccggc gctgatcggt gaacccggcg tgggcaagac ggcgatcgcc
    20281 gagggcctgg cgctgcgcat cgccaatggc gacgtgcccg acagcctgaa gcaccggaag
    20341 ctgatggcgc tcgacatggg cgcgctgatc gccggcgcca aatatcgcgg cgagttcgag
    20401 gagcggctga aatccgtgct cgacgaggtg aagggcgccg aaggggagat catcctcttc
    20461 atcgacgaga tgcacacgct gatcggcgcc ggcaagaccg acggcgcgat ggatgccagc
    20521 aacctgctga agcctgcctt ggcgcgcggc gagctgcatt gcatcggcgc gaccacgctc
    20581 gatgaatacc agaagtatat cgagaaggat ccggcgttgc agcggcggtt ccagccggtc
    20641 ttcgtgggcg agccgacggt ggaggacacc atctcgatcc tgcgcggcct caaggagaag
    20701 tacgagctgc accatggcgt gcgcattgcc gacaacgcca tcgtggcggc ggcaacgctg
    20761 agcaaccgct acatcaccaa ccgcttcctg cccgacaagg cgatcgacct gatggacgag
    20821 gccgccagcc gcatccgcat ggaagtggaa agcaagcccg aggagatcga gacgctcgac
    20881 cggcggatca tccagctcaa gatcgaggaa atggcgctgt cgaaagagag cgacgaggcc
    20941 tcgcgcgagc gactggcgac cttgcgcgag gaaatggcca atctggagca gcgcagcgcc
    21001 gagctgacca cccgctggca gaacgagcgc gacaagatcg ccgccgaagg gcgcatcaag
    21061 gagcagctcg atgccgcccg ggtggagatg gagcaggccc agcgtggcgg cgattatgcc
    21121 aaggcgggcg agctggccta tggccgcatc cccgagctgg agcggcagct ggccgaggcg
    21181 cagggcgctt ctgccaatgc cctgctgcgc gaggaagtga ccgccgagga cattgccggc
    21241 gtggtcagcc ggtggacggg cgtgccggtg gaccggatga tgcagggcga gcgcgaaaag
    21301 ctgctgcaca tggaagacga gctgggcaag cgggtgatcg gccagcggga tgcggtggtg
    21361 gccgtgtcca aggcggtgcg gcgcgcgcgg gcggggttgc aggatcccaa ccggccgctg
    21421 ggcagcttcc tgttcctggg ccccacgggc gtgggcaaga cggagctgac caaggcgctg
    21481 gccggcttcc tcttcgacga tgacaccgcg atggtgcgca tcgacatgtc ggagttcatg
    21541 gagaagcatg ccgtcagccg gctgatcggt gcccctccgg gctatgtcgg ctatgacgag
    21601 ggcggcgtgc tgacggaagc cgtgcggcgc cggccctatc aggtggtgct gttcgacgag
    21661 gtggagaagg cgcatcccga tgtcttcaac gtgctgctgc aggtgctgga tgatggccgg
    21721 ctgaccgacg ggcagggccg ggtggtggat ttcaccaaca ccatcatcat cctcaccagc
    21781 aatcttggca gccagttcct cgccaatctg gaggacgggc aaaccgccga aagcgtggag
    21841 ccgcaggtga tggaagtggt gcgtgcccat ttccgcccgg aattcctgaa ccggctggac
    21901 gagatcatcc tcttccaccg gctgggccag gaacatatgg ggccgatcgt cgagatccag
    21961 gtgaaccggg tgcagaagct gctgaaagat cgcaagatca ccctgtcgct gacccatgcc
    22021 gccgagcgct ggctgggccg ggtgggctat gatccggtct atggcgcaag gccgttgaag
    22081 cgggccgtgc agcgctatct gcaggatccg ctggccgaaa agctgctggg cggcgagatc
    22141 cccgatggca gcacggtggc gatcgacgag ggcgacgggg cgctgtccat cgccgtctcg
    22201 tgaagggatg caagggccgg gggcagggcg tgcctgcctc cggcctggcg tactgaacat
    22261 tagtgctagg tatgggcgtg gtgccttgac cggcctttcg cagcctctta gctggacggt
    22321 taaaccggcg gaaggggctg aaacgggcat gaaggcaggc gcaatcgatc agaacggggc
    22381 tgttttgccc cttgcgcgca ggggctggct gagatgaccg gccagccttc ccttgccgcg
    22441 ccaacccctg tgcccgccgc cgcgcgcgag caactgctgg ataccgccag cgcgatcatg
    22501 cgcgagagcg atacggtgga tgtctcgctg tcggaactgg cggcgcaatc gggcctcaat
    22561 tcggcgttgg tgaaatatta cttcggcaac aaggccggcc tgatgcgggc cctgctgcag
    22621 cgtgatttcg atgcgatcct gcaggcggtg gacacgctga tgtcgcgcga tatggatccg
    22681 gaggcgaagt ttcgccgcca tctcaccgcc gtggtcgaca cgtttttcgc cacgccctat
    22741 atccaccgcc tgctgatgcg cctgatccgc gaggaaccgc ccgaggaagc ccagcgtctg
    22801 gccgatacct acctgaagcc gctgctcaac gcctatgaga agctgatcag cgatggcgtg
    22861 gcggccggcg tgttccgccc ggtggatccg cagctgttct atttcaccgt gattggcgcg
    22921 gccgacaggt tcttctctgc ccggctggtg ctgcgctatt cctccgacac cgatgtgctg
    22981 gatgaggcgc tgcgcgatcg ctatcgtgcg catctgaacg atttcgtggt ggccggcatc
    23041 cttgtgccgc ggtgaaggtt gggtggggga gagggccggg gccgcaaaca aaacaactgt
    23101 aacaatctgc tggcaggcct atgtctttgc gcccctcaag cgaaaggcaa ggcacaggtg
    23161 agcacgacag gctggcatat tggcattatc ggcggcagcg gccttgccgc ggggctggat
    23221 ctggatgatg cgcgcgaggt gatcgtggaa agccccttcg gcgcgcccag cgcggcgatc
    23281 gtgcagggca gcctcaatgg catccgcttc tccttcctgc cgcgccatgg caaggggcat
    23341 gcaacgccgc cctcgctggt caatttccgc gccaatatcg atagtctgaa gcgcgtgggc
    23401 gtgaccgatc tggtggccct gtcggcgatc ggttcgctgc gcgaggaata tgcgccgggc
    23461 catttcgtta ccgttgacca gctgatcgat cgcacccatg gccgcccgtc gagcttcttt
    23521 ggcgaggggc tggtggcgca tgtcagcctg gccgatccca cctgcccgcg cctgtctggc
    23581 ctgatggcgc tggcggtgca gcatgctggc ggcatcgttc acccgcgcgg cacctatgtg
    23641 gcgatcgagg ggccgcagtt ttccacccgg gcggaaagcc gcatgtatcg ccactggggc
    23701 gccgatgtga tcggcatgac ggccttcccc gaggcgcgcc tcgcccgcga ggcggagctg
    23761 ccctatgcgc tggtgggcat ggtgaccgat tatgacgcct ggcgcgagga ggaagccggc
    23821 gtggaagcgg ccgaggtgtt cgcggtgatg caggccaatg cggcgctggc gcgcgcggcg
    23881 gtgcgcgaac tggcacaggt gctgccgcca gagcgcgcgg ccagcccgtt tgacacggcg
    23941 ctggaacatg cctttgcaac gccggctgcc gcgcgggacc cggcgctggt ggccaggctg
    24001 gatgccgtgg ccgggcgggt gctgcgccag ggctgaagca agggggcagc gcggggttgg
    24061 ccctcgcgct gccgctcagc tcttgtgcga gaaagcgtcg gcgatcgagc aggcggccgg
    24121 gccgaggatg acgacgaaca gcgtcggcag gatgaacagg atcagcggca cggtcatgat
    24181 cgcgggcagg cgggcggctt tttcctcggc gcgcatcatg cgctcgttgc gaaattcggc
    24241 cgacagcacc cgcagcgccg aggccagcgg cgtgccatag cgttcggtct ggatcatcgt
    24301 ggtcaccacg ccgcgcacat gatcgagctt cacccggtag gccaggttct cgaaagcctg
    24361 gcgccgttcg gcgaggaacg acagctcgat cgccgtcagc gcgaattcat cgcccagttc
    24421 cggataggcc cggcccagct cgcgtgccac gcggccaaag gcggcatcca cggtcagccc
    24481 cgcctcggcg cagatcacca ggaggtccag cgcatcgggc aggcccttgc ggacagcggt
    24541 ggtgcgcttg gagacgagat tgtcgatata aagatcgggg cccttgtagc cgccggcagt
    24601 ggccacggca aagatgatga agcgcttgaa cgggctccac tcgggccaca ggttcagcca
    24661 gtagacgcag atcaccacca gcaggcccag cacgatcggc accaccatgc ggccgaagat
    24721 gatggcaacg ccccattcct tgttgcggat gccggcctgg gccagcttct gctgcacttc
    24781 ggccagctgg ctgtcctgca gcaccttcag cttgttgagg atgtcgcgga tggtatcggt
    24841 ggtgtcggag cggcggacga tctgggcgcg cttcttgggg ccagacgaca tgatgccggc
    24901 cttcagttcc tcgcgccgct cgttcagcgc cttcacgcgc ttggccatgg ggtcgcgcac
    24961 ggtgagcgcg gcatagatcg ccaccagcac ggcgccggcc gccaccatgg ccagcagcgt
    25021 gccaacccag aagacatcga cgccgaacag atggggcgcg gcggggtgtt ggatcatggc
    25081 gcgcctctcc tcagatctcg aaactgacca tcttggccat gatgaaggcg cccgtgccca
    25141 tccacacaaa ggcccccagg cccaccacga tcagccgctg atcggtgaag aagccgccca
    25201 gatattccgg gttgatccag tagatcatgc cgaagacgag aaagggcagc gagccgacga
    25261 tataggcaga ggccttggat tcagagctca tcgccttgat cttcagcttc atctgggcgc
    25321 gcttgcgcag cacatcggcc aggttcgcca gcgtttcggc caggttgccg ccggtttcgc
    25381 gctggatggc cagcgtgatg cagaagaagt tgaactcggc catgctcagc cggtcggcgg
    25441 tttcctgcag cgcttcttcc atggtgcggc cgatgcggat gcgctctgtc accagcttga
    25501 attcctgggc cacgggcccg cgcacctcgc gcgagacgat gcccagcgtt tctgtcaccg
    25561 gcaggcccga gcgcaggccg cgcaccagca gctcgatcgc atcggggaac ttggaaatga
    25621 aggcattggc gcgccgctgg atgagatggc ccactaccat atgcggcacg cctgcgccca
    25681 ccgccacgcc caccgccagc gccagcaggg gcacgccgct gcgcatgtag acgaggaggc
    25741 cgatgaccgc ggtgatgccg gcgcaggtgt agagatattg ctccaccgtc cagtgcttgc
    25801 cggtctggtg caggcgcagg gcaagcgctg cccagcgcga tatttgcgca gcggcggtgg
    25861 tgtaggcggg cttgcgcgag gccagtgcct tgcgcagctg ggcctccatc ttgtcggccg
    25921 cgctgtcgga atggcggaag cgcacagcct gcagccggcg cgcgccctgc ttgccggcat
    25981 ccgggccgag gaaggcaaag cccaccagcc ccagcaccag catgccgccc accgccagca
    26041 tcaggatctg catcatgttc atggcgcgcc ctgcctttct tcctgcctca gcgcccggcg
    26101 gccttgtcct tgcgggccag caggctcttg aaatcgatga ggccgagcag cgacttggcc
    26161 tcgggcactt ccaccggcgc tgtgctgtca gaggcgttgg cgcgcacggc ctgggccagc
    26221 tcgcgcagca cggcggtggc cttgctggcc ttgttggcct cggtgaaggt ttgccccagc
    26281 ttggcggcat ggatcgccgc cttggcatcg aagggcacga catagttgat cttgcgctcg
    26341 atcgaggctt cgaaatcggc gcggccaatc tccgacaggc ccgaatagac cttgttggcc
    26401 accaccagca ccagcgcatg gccggcattg gcgcgcagcc aggaaaggat gcggatgcaa
    26461 tcgcgcgcgc cggccagggt cagctcggtc accagcacga tcacgttcac atcggccagc
    26521 acatgcggga agttgaccag catcgagcgc ggcaggtcca ccaccgtcat ctcgaagctc
    26581 tggcgaaatt cctcctccag ctggatgaag gcgctgccat cggtcatcag gggcgctgaa
    26641 atcggcgcct cggccgaaag gatcgagagg ttgtcggccg ccttgatcat cgcgcgttcg
    26701 aggaacaggc cgtcgatgcg gctggggttc tcgatggcat cggtcaggcc ccggcccggc
    26761 tccagatcga gtgccagcgc gccggtgccg aaatggatgt cgaggtcgaa cagggcggtg
    26821 ggcagctggt gatcgctggc aaacagccag gccatggcgg tggcgatggt ggaggcgccc
    26881 accccgccgc gcgtgccgat caccgcggtg gagacatggg catgggcggc cgagggatcc
    26941 tggttgcggg gcgcggcaaa caccgcctgg gcgtgctgca gcgtctcgcg cacatggctg
    27001 gccaccagcg gcttcagcag ataatcatgg atgccgctgg tcagcagttc gcggtagagg
    27061 cgcacatcgt tcacctggcc cacggcgatc accaccgtgc caggctcgca cacttcggcc
    27121 aggtggttga tctcggagag cggatcgccg ctttccgaca ggtcgaccat caggatcgac
    27181 gggctggaag agaccgacag cgtctggatg gcgttcctga ggccaccttt cacgcagcgt
    27241 tccggcggcc agcccatttc cgcgctcagc gggcgcagca catcgagcga ggcatcgtcg
    27301 cacaggaagg cggcaaaggt gtcgcgggcg gaatggctgg tgccgccggc tttccagggt
    27361 gcgttcatgt cagctgcctc cgcctgaagt ggcacttgcc ttcacattct tgccgccgcc
    27421 gccgctgggg gcagaggtgc gataggcgtt gatcgccttg tcgccgctca cctgggtggt
    27481 ctcgccggca ttgctggcgc cgtgcagcag gtcgtcgggg ttggccacca tggcggccag
    27541 attgccgttc accgcgcagc catagttgct ggaggtggca ttcttgaaat tggtttccga
    27601 attggccgac cagtcggggc agtggggcac ttcggcctcg gcgcgggtga tcaccacgcg
    27661 cacgctgccg gtgggcacat agccttcggt gaccggcgcc ttctcggcca gcggcaggcc
    27721 atagcgggtg agcacgctgg tgatgctggc gcgggtggca tggttggcca ccggatcgtc
    27781 caccgccacg gtgtcgccat agcgcagctt gagcgcggca aaccagcccg acaggcgctg
    27841 cagctcggcc atgcccaggc cattggggcc gctttgcagg tccagcgtgt agacggtgtg
    27901 gtgcaccacc ggctggtgga tgctgtcgag caggcggttg cggggctggc cgccaatgca
    27961 ggccgacagc gccagagaca gcgacaaggg cagcaccagc gccagcgcgc gccggctgtg
    28021 gcgggagaag gggagggcgg gcgttgcggg catggcggcg gcctcgttca gttgagggag
    28081 aagccgggct ggcctggggc ggccgccggg ggcgtggatg tggccggggc ggcatcgccg
    28141 gtgcgggcag caggcggggc cggggatttg gccgaggact ggggcgcggg cgcggcgatg
    28201 gccggcttgc cgccgctttc ggcagcggtg gcgccggggc gcttggcatc cttggggtcc
    28261 ttgggccggt cggccagcat gttgccgaac acgcgctgga tgtcgtcggg gctctgcatg
    28321 ccgtcggtcg gcagcttgat gtcgccatcg ttgacgggct tgaccagata gggcgtgacg
    28381 acgattacca gctcggtgtc gccgcgggca aagctggtgg atttgaacag cgcgcccagt
    28441 accggcacat cgcccgcgcc ggggatcttg gagatcgaat tggtggcgct gttgctgagg
    28501 aggcctgcga tcatgaagct ctggcccgag cccagctcca ccgtggtttc ggcccggcgg
    28561 atggtgaggg cgggcaccga ggtgccgttc atcgtcaccg cgttggaggt gtcgagctgc
    28621 gagacttcgg ggcgcacgcg cagcgagatg cggccatcgg ccagcaccgt gggcgtgtag
    28681 gacagcgaga cgccatagtt cttgtattcg atgctggtgg tgccaagccc gctggaaatg
    28741 gggatgggat attgcccgcc ggccaggaaa tcggccgtct cgccggaaag ggcggtgagg
    28801 ttgggctggg ccagcgtggt taccaggccc accgtttcgg ccgcatccag tgcggaaagc
    28861 agggtcatgc cgccaaagct ggtgagatag cccagcgtgt tgctgctggc caggccattg
    28921 agcgtgaccg aggtgccccc ggagctggcc gaggccgagg aggtgctgct gctgctgctc
    28981 gaggaggtgc tggaggtgcc ggtgctatac aggcccgagc ggccggtgag gaaggcaaag
    29041 ccgttgctgg aagagaccgt ctgcaggttc acgcccagcg cgcgggtgag cttgcgcgag
    29101 acttcggcaa tgcgcacctg caggttcacc tgcagcgggg tggcggtgtg caggcggctg
    29161 atcacattgg tgccgctgcc catataggcc tgcaccaggc gctgggcctc ggcgctgtct
    29221 tcgggggcgt tgatggtgcc ggtcagcagc acggtgttgc tgctcatggt ggaaacggtg
    29281 atgcgggcat cgggcatcgc cactttcagc atctggtcga cgctgtcgat gttgctgccc
    29341 acgcgcaggg tggaggacca gacgatctgc ccgcccggcg cgctggcata gatggtcgtc
    29401 tcgccggcgc ccttgccgaa gatgtagagc tggcggttcg atttcacctg cacatcggcg
    29461 atcttgtcat cggcgatgaa cacatcgctg accgtgccgc ccagattgac caactggccg
    29521 cgcccgatcg acagcaggat ctcgctggcc ggccgggtga tggaggcagc gcccagcggc
    29581 gccgtgcggc ctgccaccag gcgcacgccg gcatgcgggg ccgggcgatg ggccagcggg
    29641 cggcgggcgg gctgatgggc gccggggcga tgcgcgggcg cattgcgggc cagcagcacg
    29701 ccggcgccct cttcttgcgc cagcgctgtg gttggcagca ggccgatcgc ggcgatggtg
    29761 caggccagca ggcggtgggc gcaggcgatg cggggcaggc ggcggatcat tgcatggctc
    29821 ctgcaggcag ggcattgccg gccgcgttgc cggcattgcc aacgcccatg ccgatggtgg
    29881 cggtgggcgt gtagggcccg gcattgccgc cataaaggcc ggcgcggccg gtctgcacct
    29941 cggtggtgtc gcgcccgcgg gtcacggtca cgccattgcc gcggcgcatc atgccggcgg
    30001 cctggccctg gggggtgctg ctcgacatcg tcttgccggt gttggggccg gcgtggcgct
    30061 ggaagcgcga gacatcgccg ccggtggaaa agctggtggc gccctggctg gggcggttca
    30121 tggcggcggt gatcaggcgg ctttcctcct gcgggctggc gccatccttc agctttacct
    30181 ggcccgaggc gacggcatga tcgagatcgg actggttatc ggcgatggag cgcagcacca
    30241 ggctgatcgt gccgaccgac tgggccacct cgatcttctc ggcgagcttg ggggttactt
    30301 ccagcgtgac gttatggaag gggtgaacca ccgtcttgcc attggcggat tcggtttcgg
    30361 tggcctggtc ggtggccagc acgcgcaggt tgcgcaggat cgtctcggtg gcatagaccg
    30421 gccggtcgtc gctgacagag acattggccg cggtcatctg cacgctgtcc ttcaccgagg
    30481 aagtgagcat caggtccaca tggtcgcccg ggaagatgaa gccggccacc gaggtcttgt
    30541 cggacaccgg gatggtgatc gcccgcatgc ccggccccag cgccgctgcc agaaaaccgc
    30601 gatcgccggg ggccaccagc gcgccttgcg tcagcggctg gccggcggtg atgggaaagc
    30661 gcaccaccgt gcccaccagc ttgttcatcg ccaccgaacc ttcaacgaaa taggcatcgc
    30721 gcaccatgtc cttgggccat tcctgaaagg agacggcatc ggcggtgatg atcgtgccca
    30781 ccggcagcgc gcgctgcgcc accagcacct tgggcccctt gggcgccacg gccgcgggca
    30841 gggcagcggc cacttgcggc gcggcgctgc ccgagaggag gctgcgcgcg gcaagcgcag
    30901 tgccgatcgc gattaccatc gcgcccagca gcagcatcag ctttctttga tccatggcgc
    30961 aatcagcctc tttttccgat tccgcgagcc gccttgcgcc ccgtcttcat tcgttaatgc
    31021 gactataatg gttaacatcc ggtcactgcc gggcgggcct gccggggctc agcccagcgc
    31081 ggcgccggcg cgcagcagcg gcagataggc aatcgccagt atccacaggc cagcggccga
    31141 aatggcgatg ccatagggca cttccagctt ctctgtgcgg gcgccgaggc ggtggcggat
    31201 cagcatggcc agcgtcagca cgccgccggc cagcgccatc gctaccagca ggttgaggaa
    31261 gcgatcgggc cggatccaca gcgccagcgc cgccagcagc ttcacatcgc cgccgcccat
    31321 atagccgcgc gcgaacatca ggccgaaaaa cagcagggtg gccgcgctgg cgcccagctg
    31381 ccagccgatt gccggccagc caaggccagc ggcccaccaa tagagcgggg cggccagggc
    31441 aatggcgccg ttcagcttgt tgtcgatctg gcggcggcgg atatcggtga tgctggccac
    31501 caccagcgcc agcgccagcg ccgccagcaa accgcccgcg attgccccaa cctgcataaa
    31561 accccctgca aacatcgccc caaggggtaa agcgcagggc ttacgaaata gtaaccaagg
    31621 gcgcgcgcgg ggaaaggcgc cggcgggccc cttgcgcctg cgggcgggcg caaaagcaca
    31681 gcgcggcctg ccgaatcggg cttgaccgca aggcccatgc aggcttggga gaggcgatga
    31741 acaggcccac cctcccggcc ggggacagcg ccgggctttt gcaaccttac gatatcgcgc
    31801 tgatcggcgc cggcatggcc ggggcctcgc tggctgccgc gctgctagcc cgcgcgccgg
    31861 ccctcagggt ggtgctggcc gaggcggagg cgcggccggg cctccatgcc acgggccgtt
    31921 ccgccgcctt ctggacggaa agctatggcg ggccgggggt ggtgccgtta accttggcct
    31981 cgcatgccgc gctggagggg ctgggcgtgt tgcgtcggcg cggcgcgtta acgctggcgc
    32041 gggccgggca ggaagcgcag ctggaggcct ttgccgcgcg ctatcgggcg ctgggcgtca
    32101 gcaccggggt gctgggccgg gcgcaggcgg cggctttggt gccgggcctc aagccgggct
    32161 ggatcggcgg cgtgatggag cctgattgct gcgacatcga tgtcgcccgg ctgcatcagc
    32221 attatctggc cgaggcgcgg cgggcgggcg ccgatctcct ctgcggcgcc gggctggcat
    32281 cggccgagcc ggcgggcgcg ggctggcagc tgcatctggc cgatgggcgg cgcttgcgtg
    32341 cgggcgtgct ggtcaatgcg gcgggggcct gggctgatga ggtggcgcgc cgggcggggg
    32401 cggtgccgct gggcattcag ccctatcggc gcaccatggc gcaattgcgc gtggcgcccc
    32461 agcccgatgc cgacctgccg ctggtgctgg accttgacga aagcttctat ttcaagccgc
    32521 aatcgggccg cctctggctg accccgcatg acgagacgcc gagcgccgcc tgcgatgccg
    32581 cgcccgagga gatcgacgtg gccagcgcca tcgcccggct ggagcaccgc tgggcgggcc
    32641 tgcgcagctt cgcgccggat cggctgccgg cctatggctg ggatggccgc gtgccgggct
    32701 tcttctggtg cgccggccaa ggcggctttg gcatacagac cgcgcccgct ggcgccgatc
    32761 tcgccgcccg cctgctgctg ggcgaggggc cgggcgcggt gaaccccgtg ccttacgacc
    32821 cccgccgatt cgcgctggtc gattagcggc ggctggcctt ggcggccgcg tcgctggcca
    32881 gcttgtccac ccgctcgttc tcggcatggc cgttgtggcc gcgcacccat tgccaatcca
    32941 ccttgtgggg gcgggccacc agcagcatct cgcgccacag gtcctcgttg gccacaggtt
    33001 ccttcttggc gttcacccag cccttcttct gccagccgaa cacccatttg gtgatcccgt
    33061 cgatcacata tttgctgtcg gtggtcaggc gcacggtgca gggctggttg agcgccgcca
    33121 gcgcgcggat caccgcggtc atctccatgc ggttgttggt ggtttccgcc tcgctgccca
    33181 ccagttcctt ctcatgctcg cccatgcgca gcagcgcggc ccagccgccc gggcccggat
    33241 tgcccttgca ggcgccgtcg gtgaagatct ccacgtcctt catgcggcaa acaggcccgc
    33301 atgggcaggg tcggcataga attccagccg gcgggcaaag gccatcggat ccttgcgggt
    33361 aacaagggca tctgcgggcg tgtgcagcca gtcgtaggcg cgggtggcca gaaagcgcat
    33421 ggccgcgccg cgcgccagca gcggcagggc cgcgcgctct tcggccgaca gcgggcgcac
    33481 cgcttcatag ccagccagca gggcggcgga aatgccggca tcgaactggc gtccatcggc
    33541 cgagaagcac caggccgcat gggtgacggc gacatcatag gccagcgtgt cggtgcaggc
    33601 gaaatagaag tcgatgaggc cggtcacgtc ctcgcccagc atcagcacat tgtcggggaa
    33661 gagatcggca tggatgatgc cggtgggcag atgatccggc cagtgggcgg cgagcgccgg
    33721 caattcgcac gccaccagat cgcccagcgc cggatcgatg gcggcaaggc cagccgcgcc
    33781 gcagccgtcc agcaggtctc gccaggtggc aaggcccatg ccattggcgc gggccatggg
    33841 aaaatcggcc gcggccagat gcacgcgggc cagcgcctgg cccaccgcgc gggcctgcgc
    33901 gggcgtgggc cggctgagcg agacgccagg caggaattcg atcagcgcgg ccggcttctg
    33961 cccgccatcg gcaccggcca cggcatggct gagcgcgccc tgccgatcgt ggatggtgcg
    34021 cggcaccggg cagccgcgcg ccgccagatg atcgagcagc cccaggaaga agggcagatc
    34081 cgccacttcc gtgcgcgctt catagatggt gaggatgaag cgcgggcccc ccgccgggcc
    34141 gccggtcgtc tcgacaatga agttggtgtt cgagacgccc tcggcaatgc ccttgtgcat
    34201 gaggggcgcg cccacgtcgt aggcggcgat cagcgcggca aaggcgctgt cgtccagatg
    34261 cgtgtaaacc gccaatgtgc cttgccttat tcggcgtcgg tcagctggcg gggcagcttg
    34321 aagatcatct tttcctcggc ggtgatgatc gtctcctcgg tcacgctgcg ccaggtggag
    34381 ataaggtcca ccacttcgcg caccagttcc tcgggcgcgg aggcgccggc ggtgaggccc
    34441 agcgtgttca cgccctccag ccagctctcg tcgatctcgc tggcgcgctg cacgaggcgc
    34501 gagcgggtgc cgaaacgttc ggccacttcc accagccgct gcgagttcga cgaattgggc
    34561 gcgccgatca cgaaaacggc atcgcaatcc tcggcgatgg ccttcaccgc tgcctggcgg
    34621 ttggacgtgg catagcagat atcctcggcg cgcgggccgg cgatctgcgg atagcgggcg
    34681 cgcagggccg cgacgatctc ggcggtatcg tccaccgaca gggtggtctg ggtaaggaag
    34741 gccagcttgc cattgtcctc aaagggcagg gcggccacgt cttccagcgt ttccaccagc
    34801 gtcatctggc cctcgggcac ctggcccagc gtgccgatca cttcggggtg gcccttgtgg
    34861 ccgatgaaga agatgtggcg gccggcctcg atatggcgct cggcctggcg gtgcaccttg
    34921 gaaaccagcg ggcaggtggc atccagccag tccagcccgc gctcgttggc cttggccggg
    34981 atgctcttgg gcacgccatg ggccgagaag atcaccggcg cgccatcggg cacttcatcc
    35041 agctcttcca cgaaaatggc gcctttttcg cgcaggccat ccaccacgaa gcggttgtgg
    35101 acgatctcgt gccgcacata gacgggcgcg ccatatttgc gaatcgcccg ctcgacgatc
    35161 tcgatcgcgc ggtcgacacc agcgcagaag ccgcgggggg cagccaccag aagccgcagc
    35221 ggcggcagcg agggggaagg aaagccagcg ttcataatcg ggcatctagc gctttcgctc
    35281 gcacgccgct agggcggttg ggcaaggatt tcttgaggac atgcagatgc agtttcgttc
    35341 acgcctggtt tttgcgcttg ccacaggcgc cctgcttgcc ggttgttcgc aaaagggcga
    35401 gctggtggtg gacgagggcg tgggcatcgc cgccatccgc agccagtgcc cctctgtggg
    35461 cattgccgat tataccggcg atgtgaccac cttccgcgcg cccggcgcca ccgatacggc
    35521 caatatggac gtgtcggccg cgctgaccaa tgtgcgcatg aagtgcgatc aggatggcgt
    35581 gaagctggat acgcgccagg ccgaagtgcg cccgggcgag ctggtgcatg ccacggtcag
    35641 cttcgatgtg ctggcccgcc gcaccgatgg caccggcgcg cgcgatctca acctgccctt
    35701 cttcatcacc gtgctgcgcg gcagcaatgc ggtgatcgcc aagcgcatcg gcacggtggc
    35761 aatccacttc gccccgggcg agctgcgcgc caccggccat gccgaggggc tggcgctgat
    35821 cgacaagaac gaggcgacgc tgagccgcga catccgcgac cgaatcacca agaagcgcgt
    35881 ggccggcgag gctgatgccg cggttgatcc gctggcccag tcggatgtgc aggatgccat
    35941 tgcgcgcgcc accttcgagg tgctggtggg cttccagctg tcggacgagc agctgaccta
    36001 taacgccacg cgttaaggcg cggcaggcag gggcagggcc gctgccggct tccctcgcgg
    36061 ggggaaaccg gctaacggcc ctgccatgtc tgatagccaa gccctttccg ccgcgcccgt
    36121 gttcactgcc tttgccggcc atgtcgccgc cgcgctcgat cgcctggagg cggcgggcac
    36181 gctgccggcc ggccttggcc gcgcggccgt cacggtggag ccgccgcgcg atcctgccca
    36241 tggcgatctg gccaccaatg ccgccatggt gctggccaag cccgccggca ccaacccgcg
    36301 cgcccttgcc accgcgctgg tggccgagct ggagaagctg cttgaagttg ccagtgcgtc
    36361 catcgccggg ccgggcttca tcaaccttac cctggcgccg gccgcctggg tggaggaaat
    36421 ccgcaagatt gccgcgctgg cgggcgatta tggccgcagc gcgctgggcg gcggcaccac
    36481 ggtcaatgtc gaatatgtca gcgccaaccc caccggcccc atgcatatgg gccattgccg
    36541 gggcgctgtg gtgggcgatg cgctggccag cctgctggaa tttgccggcc acaaggtgat
    36601 ccgcgaatat tatgtgaacg atgccggcgc gcaggtgcag gtgctcgccc gttcggccca
    36661 tctgcgctat cgcgaggcgc tgggcgaggc agtgggcgag attcccgccg gcctctatcc
    36721 gggcgattat ctggtgccgg tgggccgcgc gctggccgag gagttcggcg atcgctatgc
    36781 ccgggcgccc gaggcggaat ggctggccct gttccgcgtg aaggcggtgg ccgcgatgat
    36841 ggcgatgatc cgcgccgatc tggcgctgct gggcatccgc cacgatctgt tcagctcgga
    36901 agcagagctg caggccgccg gcaagcccga ggaggccgag gcctggctgc gcagtgtcga
    36961 tctggtctat gatggcgaga tggaagcgcc caagggcaag acgcccgacg actgggagcc
    37021 ggtggcgctg cccttgttcc gctccaccaa attcggcgac gaccaggacc gcccgatcaa
    37081 gaagagcgac ggcaactgga cctatttcgg cgcggacctg gcctatcact accagaaggc
    37141 cgccactgcc gatgcgctgg tggacatctg gggggcagac catgccggca cggtgaagcg
    37201 catcaaggcg gcggtggcgg cgatgaccgg ccgcgatggc gcgccaaagc ccttcgaggt
    37261 gaagctggtg cagatggtgg cgctgctgaa gggcggcgcg ccgttcaaga tgagcaagcg
    37321 ggcgggcaat ttcgtcacgc tggccgatgt ggtggaaatg gtgggcaagg atgtggtgcg
    37381 cttcaccatg ctcactcgca agcccgaggc gcagatggac ttcgacttcg acaaggtggt
    37441 cgaggcgtcg aaggaaaacc cggtgttcta tgtgcaatat gcccatgcgc gcatttccag
    37501 cacgctgcgc aagggggcgg cggaaggctt tgcgccggcc gccgccgcgc tggagctgct
    37561 gggcacagaa gagctggagc tggtgaagct ggccgcgcaa ttcccccggc tggtggaagc
    37621 cgcagcgcag gcgcgcgagc cgcatcgcat cgcctttttc ctgcaagatc tggccgccgc
    37681 cttccacgcc tactggaatc tgggcaacga ccggcccgac aagcgcttta ttcttggcga
    37741 taacccggcc ttaaccggtg cgcgtctttt cttgggggcg caaatcgggc agattattcg
    37801 taacggcctc gctgttctgg gggtggccgc agtagaggaa atgtgaagcg atgaacgggg
    37861 ggcgttggca aacgggcgaa ggatatgggc aggcgcccgg cgcttttgcc ccctcggtgg
    37921 caggccaggg gctggcgccg ggttctggcg ggtcggatgc cggcggggcg ggctgggtgc
    37981 cctatccggc gggcggcgcc tttgcgggcc atcccggcgc agatcatgcg ggcggtgatg
    38041 cccatggcgc gccgcttgcc gaggacggcc acgcggcccc ctatcacgat gcctggctgg
    38101 agcatggcga ggaccgccac gccgccagcg ggcaggaaga gctggatctg gccggcagcg
    38161 cccgcttgcc ctggctggaa aacggcgagg attacgagga tgacgagccc gcgctcgaca
    38221 cccgccgggt gattggtgcg gccgtggcgg gcgtggcgct gctggcggcg gtggtcggcg
    38281 gggtgtggtg gatcagccac cgccacgatg ccgaggccgc gcagggcgat ggcagcctga
    38341 tcgccgcgcc caccgccccc tacaaggtgg cgccggccaa ccccggcggc aaaacctatg
    38401 acggcaccgg cgataccagc tttgccgtct cgcaagggca ggtgcgccag ggccagctgg
    38461 ccgatggcgg cccggccggc gtgccgcagg gcgcagcctc gccgcagctg cagccgcagc
    38521 ccagccccag cgccgctgcg ggccatgccg ccgagggcca tgttgctgcg caaggggcag
    38581 acaatgccca ggccgcctgg gcgggcgccg tggtgcaggt gggcgcctat tcccagcgcg
    38641 cgcaggccga agcggcctgg ggccggctgt ccacgctcaa tccgccgctc aagggcctgc
    38701 atcaccgggt ggtggaaggg caggccgata tcggcaccgt ctaccgcctg caggcgttga
    38761 ccggggcggg cggcggcggc gccctgtgcg aacagttgcg cggcgcgggc gtggcctgcc
    38821 aggtgaagca ctgagccaag tgaagcactg agggggcgcc ttctcgccgc tttccacatc
    38881 cgcatcgctg cgcccccctt gcgcgagggg cgcccggctg cgagcgtgcc cggcatgatt
    38941 cccgccattt tcggcctgtc cggccccgtt ctcaccgcta acgagcgcgc cttcttccgc
    39001 gatgcggatc cggccggcta catcctgttc ggccgcaatg tggaagggcg cgcgcagctg
    39061 cgggcgctga cggatgatct gcgcagcctg catggccgag accggctgct gatctcgatc
    39121 gatcaggaag gcggccgggt ggcgcggatg aagccgccgg tgtggccggc ctggcccgca
    39181 ggcgcgccct ttgcggccct gtgggaggcc gcgcccgcca gcgcgatcga ggcggcgcgg
    39241 ctgaacgcgc gggcgctggg gatggatctg gcagaagccg ggatcagcct tgattgctgg
    39301 ccctgcctcg atgtgcgggt gccgggcgcc catgatgtga tcggcgatcg cgcgctgggc
    39361 gaggagccgg tgcgggtggc cgcactgggc cgcgcggtgc tcgatgggct ggcggcggcc
    39421 ggcgttgtcg gcgtggtgaa gcacatgccc ggccatggcc gcgccggggc cgacagccac
    39481 aaggaactgc ccaccgttgc cgcttcgccg gccgatctgg cgcaggatct cgccccgttc
    39541 cgcgcgctgg cgcaaaaggc gctggtgggc atgaccgggc atatcctctt ccccgcctgg
    39601 gatgcggtgc acccggccac ccagtcggcc cggatcatcg gcgagatcat ccgcggcgag
    39661 atcggctttg atggcctgct gctgtcggat gatatcgaca tgcaggcgct ttcgggcacc
    39721 attcccgaaa gggcggcggc ggcgatcgcg gcgggctgcg atctggtgct caattgctgg
    39781 gcgaagatgg acgatatgca gggcatcgcc gaggcctgcc ccaccatgcc tgccggcgcg
    39841 cccgaaaggc tggcgcgggc gctggcgccc ggcgcagtgc cggccgcgcg cgatgcggcg
    39901 ctgcaggccg aattatgcgc cgcgcgcgat gcgctgctgg cgctggcgcc ggcgtgaacg
    39961 cgcctgccgc cccacagcca gagcgcggcg aggcgctcta tctggagctg gagggctggg
    40021 aggggccgct cgatctgctg cttgatctgg cccgccggca gaaggtggat ctgcgcagta
    40081 tctcgatcct cgaactggtc gatcaatatg tcggctatat cgcccgggca gaggcgctga
    40141 agctggaagt ggcggccgat tatctggtga tggcggcctg gctggcctat ctcaaatcgg
    40201 cgatgctgct gcccagggac gagcaggaag accccagccc cgaggaactg gctctgcgcc
    40261 tgcaattgcg cctggcccgg ctgggcgcca tgcgcgaggc cgccgcccgg ctgatggcgc
    40321 gcgaccggct gggccgcgat gtgttcgcgc gcggcgcgcc cgaaggcctg cgggccgata
    40381 aacgggccct gtggcaatgc agctggttcg atctggtgca ggcctatggc cgggtgaagg
    40441 cgcgcacgat gccggttgtc cacatggtgc gcgagcggcc ggtgatgacg ctggaggcgg
    40501 cgctggcccg cgtttcggcg atgctggaag tggagctgga ctggacggag ctgagcgcct
    40561 tcctgccggc gggcgagggg gcatgggcgg atctgcggct caggcgttcg gcgctggctt
    40621 ccagcttcgt ggcggcgctg gaactggcca agcaaggccg ggcggaaatc tggcaggagg
    40681 tgccctttgg ccccctgcgc ctgcgcccgc tggcgccaac aagcgcggtg ccatgatggg
    40741 ccgggcgcca tgagcgagga catcacccgc gcggtggaag cggtgctgtt tgccgccccc
    40801 cagccgatga gcctggccga gatcgccacc catctgggcg gcggcgatgt gcgcgccgcc
    40861 cttgcccgcc tgtcggccga ttatcgcggg cgcggggtgg agctggtgga gcgcggcggc
    40921 cgctggcact tccagaccgc gccggacctt gcccatctgc tgcggcgcga gcatgaggag
    40981 ccgcgccgcc tgtcgcgcgc ggccaccgaa tgcctggcca tcatcgccta tcacgagccg
    41041 gtgagccgcg ccgagatcga ggcgatccgc ggcgtgcaga ccgcgcgcgg cacgctggat
    41101 gtgctgatgg aggccggctg ggtgcgcgct gccggccggc gcgaggtgcc cggccgcccg
    41161 gtgatctatg cgaccacgcc ggcgttcctt gcccattttg gcctggccag ccgcaaggat
    41221 ctgcccggca tcgaggaatt gcgcgcggcc ggcctgctcg atccggtgga ggaagcgctg
    41281 gaagaggcgc tggccggccg ccccgagagc ccggcgcccg gcgcatagca ggcgtgcacc
    41341 ctatgccgcg cctatgccac tggcgcccgg ggaacaaagc gcctaaggaa gaataaataa
    41401 gggggcgtgt tggccttcgg gccggctctg gtcgcttgtt gcgcggccgg cgctcttttg
    41461 aggagatgtt tcgatgggtt tgtcgcttcc gcatctgatc gttctggcgg tggtcgtgct
    41521 ggttctgttc ggccgcggcc gtatttccga aatgatgggc gattttggca agggcatcaa
    41581 aagcttcaag gaaggcatgg cggatgagac gaccaaccgc ccggtaaccc cgccgcccgc
    41641 gcagattgcc gcgcaaaacg cgcccgcgcc cggcgttgcc ccccaggctg cagccacgcc
    41701 cgccaccccg gcagagcccg gccagcccgg ccagggctga gccgcttcaa ggccccgcct
    41761 gcgtgctgct ggatatcgcg ccttcggaaa tgctgctcac cctggcggtg gcggtggtgg
    41821 tgatcggccc caaggacatg ccgcgcgccc tgcgcacggc gggccgctgg attggcaaga
    41881 tgcgccgggt gtctggccat ttccgcgccg gcctcgagaa tatgatccgc gaggccgaga
    41941 tggaggagat ggagcgcaag tggcgcgagc agaatgccgc gatcatggcc gcccatccgc
    42001 cggtggcaga gcctgccgcg ctgaccggcc cggcgcccga tgcggcgctg gcagggcccg
    42061 cgtccacgcc cacgcccgcg cccatatcta cgcccgcgcc cgcccatggc ggcgatgcgc
    42121 cgccgcccca cgctccggcc tgatttcctc attttttcgg ggcgtttctg cccccctgct
    42181 gcaggcacca tggcattcga gatcaaggac atcgacgaca cgcaggcccc cctgctggag
    42241 catctgatgg agctgcgcgc gcggcttatc cgctgcctgg tggcgctggg gctgggcttt
    42301 ctcgtctgct ttgcctttgt gaagcagatc ttcgcctttc tcgtgcgccc gctggccgcc
    42361 gcctttcctg atggccaggg caggctggtc tacaccaagc tctacgaggc ctttttcgtc
    42421 gagctgaagg tggcgctgtt tgccgggctt ttgcttgcct tcccggtcat cgccaaccag
    42481 ctgtgggcgt ttatcgcgcc cgggctctat gcgcgtgaga agcgggcctt cctgcctttc
    42541 ctcattgcca cgccggtgct gttcctgtcg ggcgcggcgc tggcctatta tgtggtgatg
    42601 cccaatgcct ttcgctggct gctgggcttt ggcggctcgc tgggcgggct gaagctggag
    42661 gccttgccct ccaccggcga ttatctcgat cttgtcatgc agttcatctt tgcctttggc
    42721 gtcagctttc tgctgccggt gctgctgatg ctgctgaacc gggcggggat cgtcagccgg
    42781 gcgcagctga aggcggcgcg gcgctatgcg atcgttggca ttgtggcggc agcggcggtg
    42841 ttgacgccgc ccgatgtcgc ctcgcagctg atgctggcgg tgccgctgct gatcctgttc
    42901 gagggcacgc tggtcgtcat gtggtttacc gagcggcggg cggccgcgcg tgcttcggca
    42961 ggcccggaag aaccggccga gcccgagatg ccgctgccct gaggatcagg ccgcgggccg
    43021 ctctgccgca tagacctgcc ggccgctgac ccacacctgc cagccgccag cacccgcgcc
    43081 atcgccgccg gtgaggatga aatcggcata aaggccggtg gcaatccgcc ccagccggct
    43141 ttcggcaaag gcgcgacggg cggcttcggc attggcggcg gcctgtgcgc gggcggcctc
    43201 ctcgccgggg ggggcggctt cggcggcagg ttccagcacg ggctctgcct cctcgatgcg
    43261 ccagcgccgg tcgccggtat aggtctcgcc cagatcggcg atggctttct ccacatcggc
    43321 gcgcagcagc gggggcagtg ccggcggcgc gggcgcggcc aggcggcggg cgcggggcgc
    43381 gggcagggcg gcgggc
//
LOCUS       DASRYE010000257        13909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_157142,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000257 DASRYE010000000
VERSION     DASRYE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157142"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="VFF98_13235"
     CDS             complement(<1..1783)
                     /locus_tag="VFF98_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU64639.1"
                     /translation="MSIAQHGRGWKGPALGLLLAGVALPTAAFADDDDAAAGHEALVV
                     TGQQTKAPTPVTLSVAPTAGIAAVYSLNQQDISGLVVTTANDLLRAIPGVEVANLGNG
                     GIPNGVTIRGWGYVSDGTSVRAVIDGATNNFASGPNANGSDDLNILIPEIIGAIDVIK
                     GPFDTRYAGNYAYAGTAIFSTIDSAPNRVAISGGSWGKARLVATLGNGQAPGPTKFYI
                     AVDALTESGRRDNNSQNKLNIFAKLTQQVGAKDVLKITGQLYTDVFGQPGYIRTDLVQ
                     QGLISETSATDNATKGWRRSQGATLEWQHRDSTFNFDANAYVQHIDQYRSIERYSVVD
                     TSNNYYPENAFHDKRWTVGLGFNPWVNFKVLGIDTIFRAGGDLRGDFINTVRAPAVNN
                     QYLAQPTLLDAWTSFFNYTNGRVWNPDLYGELSLKPASWIKITGGVRDDWFNYNAQVT
                     VYSGTATGVSSPYAPGAPVGTAASLATYRFNGWSDKPTLHGGIAIVPGGGFTILGNFG
                     EGITSQWLNQVTAKSGVNYPTLYLNPGLAPTKLNTKEVVVKYANEKLGLNIEGGVYST
                     LNQGELGTDTATGNPVNLGKSVRRGWDV"
     gene            complement(2090..3505)
                     /locus_tag="VFF98_13240"
     CDS             complement(2090..3505)
                     /locus_tag="VFF98_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU64640.1"
                     /translation="MPDTPSTDTPLLAADLLITGAHIITMNAAREVYADGALAIAGSR
                     IVAVGRSADVARRVKPRATRDARGFVMTPGFIDGHIHITGDPLTRGLVRNTPDDNWGD
                     NLSKWVIPLFKAQTPAEEALSARLAALAMMRAGTTSFVEAGTVLHMAAVMDGLADTGL
                     RGRVGQWVEGRAWDPAADATAVADHAIGLLRDEVERWPDDGETLLAAWPVLVGHSTNP
                     DPVWIAARDLARQHGLKVSAHMSPRQADPDWFLARYGRRPLEHLADIGVLGPEVCLTH
                     LAAIDHAELDLLVDMGAGAIHCAHAAFLGGMGLARHGLHPEMLARGVPLMLGTDGMAA
                     DILSSARLMAAGYRDARADQYLLPATTMLEMMTVNGAQTLGWADRLGSLAEGYAADLV
                     LHDTRLPEWGGPVFDAVGQLAISAPPSGVHSVWINGRQVLEAGRNLMMDEDKLLADAV
                     QAGHAVVARTGLPARTPWPVL"
     gene            complement(3508..4119)
                     /locus_tag="VFF98_13245"
     CDS             complement(3508..4119)
                     /locus_tag="VFF98_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZU64641.1"
                     /translation="MTQSAASTRIADYPIDPDILTRWSPLAFDGTALAEADLASLFEA
                     ARWAPSAFNYQPWRFAYALRGDAQWAAFLGALLPFNLPWAEKCGALVFILSDKLVAFP
                     GKAELVPAITGSFDAGAAWAYMALQAHKQGLITHAMAGFDAALASAVTGAGSRYRIET
                     AVAIGRQGDAASLPEGLRAREVPSGRLPISALAFAGALPPCEA"
     gene            complement(4129..5208)
                     /locus_tag="VFF98_13250"
     CDS             complement(4129..5208)
                     /locus_tag="VFF98_13250"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZU64642.1"
                     /translation="MTVIDSLRPVEGAPAWRGDQLAGTNVWIYELNPAQLEELETLGA
                     RFVEESPDLRTVQAADYPLVACAEAARAWGHDVDYGRGFVLVRGLRSHLYSDALSCAI
                     YYILGLHMGDPIRQNEMGDLIDHVYATSDKTMDDPTALSSKVRDKLVYHSDSSDVVAL
                     MCLRPSREGGLSCLVSGAEIYNEILRRRPDLAPLLLEPFHWDWLKQDHSAPANTYTSP
                     IISLKDGVFSMYAGSLYILTAQDYPEVPRLTPEQIEVLELFDEITYEPGMAIEMDFRP
                     GDIQWLSNYSALHARTSFTDWPEPQRKRHLLRLWLSRDAGRPVVDGFGKNGVVQVRQA
                     PRDGAEEHPDAHFDILSISVPRLIS"
     gene            complement(5319..7145)
                     /gene="atzF"
                     /locus_tag="VFF98_13255"
     CDS             complement(5319..7145)
                     /gene="atzF"
                     /locus_tag="VFF98_13255"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HZU64643.1"
                     /translation="MIPSPPETRMQLPALPRIADIAPQVARGAWTAEGVARAMLARIA
                     AYERIQPELWISRFDEERLLAIARGIDARVAAGEDLPLAGVPFAIKDNIDLAGLATTA
                     GCPAFAYRPEDSAHVVARAIAAGALPLGKVNLDQFATGLVGTRSPYGPCRAVHNRAYI
                     SGGSSSGSAVAVAAGLAAFALGTDTAGSGRVPAALNGLVGLKPTKGRWSSSGLVPACR
                     SLDCITVFAHDVADAALVDACLAGFDAADPYARRQPASPPAFAAKAARIGLPLAHQRQ
                     FLGDGASAALYEAAIARVAATGARLVEVDITPLMEAARLLYQGPWVAERTAALADLLR
                     DGPQHIHPVVRDIVRAGQAMSAVEAFRGEYALKALARQAEPIFEAVDALLLPTCPTTY
                     RLAEMLAEPFALNANMGLYTNFVNLLDMAAIAVPAGFRANATGFGVTFIGPAFADRAL
                     VDLALAYEEIAPMPEIPAPDLAGVPQTVKLAVVGAHLAGMPLHWQLTSREAGLVARTH
                     TAPDYRLYALAESTPPKPALVHAGATGTGAAIEVEVYELGVAAFGSFVVDVPAPLAIG
                     TVTLASGEEVKGFVAEPRALEGASEITHLGGWRAYIASLAAA"
     gene            complement(7142..8143)
                     /locus_tag="VFF98_13260"
     CDS             complement(7142..8143)
                     /locus_tag="VFF98_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64644.1"
                     /translation="MASLSPAAPWPAAQEEPRERAWRFSSDEYAPAQRRKAWARTMER
                     LRLPQGVVAEGADFHASVICRTTPMGMELALLSGSAQEIAGRNPHQPAAIWLTLLLEG
                     TAELDDGDGLRPMRPGDIAYGPTGVDAALRLSSAFRLLFISIPQVALTHRIVAARQLG
                     IGLVAGDEGLGAIYSALLRAVAGGLDALGSDQLRPIEVALAEFLVAYLADRASPALDI
                     GRSRAKAQLHRIRQAIEALLGEPELTLAQVAEESGVSPRYLQKLFAAEGLNFTFYLRL
                     RRLERCRLELASPIYAGLSISQISFNWGFNGSSHFSRAFRAQYGLSPRAYRKQALGR"
     gene            complement(8163..9350)
                     /locus_tag="VFF98_13265"
     CDS             complement(8163..9350)
                     /locus_tag="VFF98_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU64645.1"
                     /translation="MTASFLDDRFFAALETSVAALEEAETLPPACYTDAAFYEFEKEA
                     LFNHEWLCVGREEQVKAAGDYFTTSIVGEPLVIVRDRAGQVRALSSVCQHRAMLVAEG
                     EGNTRGFVCPYHHWTYDLDGKLVGAPAMDRTCNFDKQSFNLPELKVEIWQGFVFVNFD
                     AEAAPLAPRLATIESHIAGYDLPSAELAQKFTGDFAWNWKVMFENNNDGYHASKLHQG
                     PLHDFIPSHLASFPEADPDDAGFLRLNGALHQDPSFNPTQKAILPVFPGLTDEERGRV
                     TFANVPPTLSLVMTPDMVIYLILRADGPEQMAQDTGILVAPGAQKDPGFEHRLEAIVN
                     SAAKIIAQDMHVDELVQVGLRSRFAVRGRYSWQEGAQGQFNHWLVRRYRRAWQALRQP
                     VDA"
     gene            9602..10555
                     /locus_tag="VFF98_13270"
     CDS             9602..10555
                     /locus_tag="VFF98_13270"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZU64646.1"
                     /translation="MLVNGKWIENWQPVQGTDGKGGFVRQSAGFRHWVTPDGRPGPSG
                     EGGFAASAGRYHLYVALTCPWASRVLFARQLKGLTEAIGVTVVEPVLTEQGWRFGDYP
                     GADRDTLNGATYMHELYTLADPAYTGRATVPVLWDTERRTIVNNESSELLRILNTGFG
                     DLASDEVDLYPEALRTAIDALNEEIYPRLNNGVYRAGFATTQEAYEEAFDDVFGMLDA
                     LEARLAAGGPYLLGAALTEADIRLFVTLVRFDAAYHGIFKCNLRRLADYPALSAYMLR
                     LLALPGARATVNIDHIKQGYYSIRALNPNGIVPKGPALAGF"
     gene            complement(10626..11114)
                     /locus_tag="VFF98_13275"
     CDS             complement(10626..11114)
                     /locus_tag="VFF98_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64647.1"
                     /translation="MTDVNHSIGPKRPTWTHMALCVRDVDATASWYEAFTHLRVLARH
                     ADKDGKNVWLADPDQKDRPFVLVMGQFYEGHDPFAPAAHPPLGPFAHIGIELPSKEAV
                     DAIAAKGREGGCLALGPLQMPKDIGYICFLKDPDGNTLEFSFDQGVFETARDVWGSEV
                     SA"
     gene            11505..13877
                     /locus_tag="VFF98_13280"
     CDS             11505..13877
                     /locus_tag="VFF98_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64648.1"
                     /translation="MCAAALAGAAPAMAAASTAANTADAAASTAEAAPDSQGARQGLG
                     EITVTAERRVISLQKAPIAISAINADSLKASNIVDVTGLNGSVPGLVVANSGGGEKMI
                     SIRGIGSQTPENTDTQPGVSYHVDGVYIFNSIAANAAFIDVAQVEVLRGPQGTMFGQG
                     STGGTINVVSNQPKLGKFSGEGEIGGGNYGYFMGKGAINVPIGDTIAIRAAIDHVGHE
                     GYSKVSSVAGNANFREDDANNTGWRVSALWQPAPNFSLTFNTIQYRSLTHGQAMKNAL
                     DPNSDPREVTQDYPGRTYISTALYSLTAKWDTPFATVRSITSYQNMHSELSWDADGLT
                     PSLFYAETYSPATYVGYNYDHVALWQSDVNSVTQEVNLTSNRKGPLEWIVGGVYLHSK
                     NTQYINEYKADDSNPLRPALAESTAYNDAAVSALTYADLAEITREEEAAYFQLTWNAT
                     DKLTFIGGARFNHDRYTGAADSFSNGTPDVTSGAYLQMKPTKGYATSVWTGKAAVQYK
                     LTPTNMVYFSYTRGFKPGGINATASAGGSDLIDANYKPEIVDSLEIGSKNRFMGDTLQ
                     FNASGFYYFYKDMQFLDSDPQLYGYGISNVGLAHIYGLELETAWLPSKHWKLEGSASF
                     LHGEVASHTTGLEAGQALAAQDAAGYTGYLYWYNTTAAAAARAGAEQDLYGKKVPGMP
                     SLQGNIALSYMNRVGPGDLTARIQYLYRGGYQARVFNTAFDAVPAYDQVNLMLHYKPD
                     HLPIDITLRVTNLFNEAGVNSRYTDPYGSAQSFTTYIPPRQFIGSVGFRF"
BASE COUNT         2414 a         4736 c         4520 g         2239 t
ORIGIN      
        1 ccacatccca gccccgccgc accgatttgc ccagattgac cggattgccg gtggccgtat
       61 cggtgcccag ctcgccctgg ttcagcgtgc tgtaaacgcc gccctcgatg ttgaggccca
      121 gcttctcatt ggcatatttg accaccactt ccttggtgtt cagcttggtg ggggccaggc
      181 cggggttgag atagagcgtg ggatagttga cgcccgattt cgccgtcacc tggttcagcc
      241 attgcgaggt gatgccctcg ccgaaattgc ccaggatggt gaagccgccg cccggcacga
      301 tggcaatgcc gccatgcagc gtcggcttgt cggaccagcc gttgaaccgg taggtggcaa
      361 gcgaggcggc cgtgcccacc ggcgcgcccg gcgcataggg cgaggaaacg ccggtggcgg
      421 tgccggaata gacggtgacc tgcgcgttat agttgaacca gtcatcccgc acgccgccgg
      481 tgatcttgat ccacgaggcc ggcttcagcg acagttcgcc atacagatcc gggttccaca
      541 cccgcccgtt ggtgtagttg aagaagctcg tccaggcatc cagcagcgtg ggctgggcca
      601 gatactggtt gttgaccgcc ggcgcgcgca ccgtgttgat gaaatcgccg cgcagatcgc
      661 cgcccgcccg gaagatcgta tcgatcccca gcaccttgaa attcacccag gggttgaagc
      721 ccagccccac cgtccagcgt ttgtcgtgga aggcgttttc ggggtaataa ttgttgctgg
      781 tatccaccac ggaatagcgc tcgatcgagc gatactggtc gatatgctgg acataggcgt
      841 tggcatcgaa gttgaaggtg ctgtcgcggt gctgccattc cagcgtggcc ccttgcgagc
      901 ggcgccagcc cttcgtggca ttgtcggtgg ccgaggtttc gctgatcagc ccctgctgca
      961 ccagatcggt gcggatatag ccgggctggc cgaacacgtc ggtgtagagc tggccggtga
     1021 tcttcagcac atccttcgcg cccacctgct gggtcagctt ggcgaatatg ttgagcttgt
     1081 tctgggaatt gttgtcgcgc cggccgcttt cggtcagcgc gtcaacggcg atgtagaatt
     1141 tcgtcggccc cggggcctgg ccattgccca gcgttgccac cagccgcgcc ttgccccagg
     1201 atccgcccga gatcgccacc cggttggggg cgctgtctat cgtcgagaag atcgccgtgc
     1261 cggcataggc gtaattgccc gcatagcgcg tgtcgaacgg ccccttgatg acatcgatcg
     1321 cgccgatgat ctcggggatc aggatgttga ggtcgtccga accgttggca ttggggccgg
     1381 aagcgaaatt gttggtggcg ccatcgatca ccgcgcgcac cgacgtacca tcgctgacat
     1441 agccccagcc gcggatcgtc acgccattgg gaatgccgcc attgcccaga ttggccactt
     1501 ccacccccgg aatggcgcgc agcagatcat tggccgtggt gacgacaagg ccggaaatat
     1561 cctgctggtt cagcgaatag accgccgcga tgccggccgt gggcgccacc gacagggtga
     1621 caggcgtggg cgccttggtc tgctggccgg ttaccaccag cgcctcgtgc ccggcggcgg
     1681 cgtcatcgtc atctgcaaag gcggccgtgg gcaaggccac cccggccagc aacaggccca
     1741 gcgccggccc cttccagccg cgcccatgct gtgcaatgct catgctactt tctccgatta
     1801 tcccgatagt tggcgcgcgg ccgcggccgc atgcgataag gcgcaaacgg cctgcctgct
     1861 ggctgccgcc ccctgctggc cgatcgggaa atccggtgcg gcacaaaggc cgccagctcc
     1921 cgtccggttg ggagctgtgt atatcctagt taatagatgg gaatttagat tttcttttgc
     1981 gcgctaaaga ggggccttgc catcgccctt tcgcacggcc gcaagcgggc ccacatggcc
     2041 ggcaaacctg ccagccacgc ggcccgcttg cggcagcccc ggggcgcccc tagagcaccg
     2101 gccagggcgt gcgcgccggc agccccgtgc gcgccaccac ggcatggccg gcctgcaccg
     2161 catcggccag cagcttgtcc tcgtccatca tcaggttgcg gcccgcttcc agcacctgcc
     2221 gcccgttgat ccacacgctg tgcacgcccg atggcggcgc gctgatggcc agctgcccca
     2281 ccgcatcgaa caccggcccg ccccattccg gcaggcgcgt gtcatgcagc acaaggtcgg
     2341 cggcatagcc ttcggccagg ctgcccagcc ggtcggccca gcccagcgtt tgggcgccgt
     2401 tcaccgtcat catctccagc atggtggtgg ccggcagcag atattggtcg gcccgggcat
     2461 cgcgatagcc ggccgccatc aaccgggccg aggagagaat atcggccgcc atgccatccg
     2521 tgcccagcat cagcggcacg ccgcgcgcca gcatctccgg gtgcaaccca tggcgcgcca
     2581 gccccatgcc gcccagaaac gccgcatggg cgcaatggat ggcgcccgcg cccatgtcca
     2641 ccagcagatc cagctcggcg tgatcgatgg cggcaaggtg cgtcaggcac acctccggcc
     2701 ccagcacgcc gatatcggcc agatgttcca gcgggcggcg gccatagcgg gccaggaacc
     2761 agtccgggtc ggcctggcgc gggctcatat gggcggatac cttgaggcca tgctggcggg
     2821 ccagatcgcg cgcggcaatc cacaccggat ccgggttggt ggaatggccc accagcaccg
     2881 gccaggcggc cagcagcgtc tcgccatcat cgggccagcg ctccacctca tcgcgcagca
     2941 ggccaatggc gtggtcggca acggccgtgg catcggccgc cggatcccag gcgcggcctt
     3001 ccacccattg gcccacgcgg ccgcgcaggc ccgtgtcggc cagcccgtcc atcaccgcgg
     3061 ccatgtgcag caccgtgccc gcttcgacaa agctggtggt gccagcgcgc atcatcgcca
     3121 aggccgccag ccgggccgac agcgcctcct ccgccggcgt ttgcgccttg aacagcggga
     3181 tcacccattt ggagagatta tcgccccaat tgtcatccgg cgtgttgcgc accagaccgc
     3241 gcgtcagcgg atcgccggtg atgtggatat ggccgtcgat gaagccgggc gtcatcacga
     3301 agccgcgcgc atcgcgggtg gcgcgcggct tcacccggcg cgccacatcg gcgctgcggc
     3361 ccaccgcgac gatgcggctg ccggcgatcg ccagcgcgcc atcggcataa acctcgcgcg
     3421 cggcgttcat ggtgatgatg tgggcgccgg tgatcagcag gtcggcggcg agaaggggcg
     3481 tgtcggtcga gggcgtgtcg ggcatcgtca tgcctcgcaa ggcggcaggg cgccggcaaa
     3541 ggccagcgcg ctgatcggca ggcggccgct gggcacttca cgcgcgcgca agccctcggg
     3601 caggctggcg gcatcgccct ggcggccgat ggccacggcc gtctcgatcc ggtagcggct
     3661 gccggcgcct gtcaccgcgc tcgccagcgc ggcatcgaag ccggccatgg catgggtgat
     3721 caggccctgc ttgtgcgcct gcagcgccat ataggcccag gccgcgcccg catcgaagct
     3781 gccggtgatc gccggcacca gctctgcctt gccgggaaag gccaccagct tgtccgacag
     3841 gatgaacacc agcgccccgc atttttccgc ccagggcagg ttgaagggca gcagcgcgcc
     3901 cagaaacgcc gcccattgcg catcgccccg cagcgcatag gcaaagcgcc agggctgata
     3961 gttgaaggcc gaaggcgccc agcgcgccgc ctcgaacagc gaggccagat ccgcctcggc
     4021 cagcgccgtg ccatcgaagg ccagcggcga ccagcgggtg aggatatcgg gatcgatggg
     4081 ataatcggcg atgcgcgtgg aagcggcgct ctgggtcatg ctgcgtcctt acgagatcag
     4141 gcggggaacc gagatcgaca ggatgtcgaa atgggcatcg gggtgttctt cggcgccatc
     4201 gcgcggggct tggcgcacct gcaccacgcc gttcttgcca aagccatcca ccaccgggcg
     4261 gccggcatcg cggctcagcc acaggcgcag caggtggcgc ttgcgctggg gctcgggcca
     4321 atcggtgaag gaggtgcggg cgtgcagcgc cgaatagttc gacagccact ggatatcgcc
     4381 ggggcggaaa tccatctcga tcgccatgcc gggctcgtag gtgatctcgt cgaagagttc
     4441 gagcacctcg atctgctcgg gggtaaggcg gggcacctcg gggtaatcct gcgccgtcag
     4501 gatgtagagc gagccggcat acatgctgaa gacgccatcc ttcaggctga tgatcggcga
     4561 ggtgtaggtg ttggccggcg ccgaatggtc ctgcttcagc cagtcccagt ggaagggttc
     4621 gagcagcagc ggggccagat cagggcggcg gcgcaggatc tcgttgtaga tctcggcgcc
     4681 cgaaacgagg caggaaagcc ccccttcgcg gctggggcgc aggcacatca gcgccaccac
     4741 atcggaactg tcggaatgat agaccagctt gtcgcgcacc ttggaggaaa gcgcggtggg
     4801 atcatccatc gtcttgtcag aggtggcgta gacgtggtcg atcagatcgc ccatctcgtt
     4861 ctggcggatg ggatcgccca tgtgcaggcc caggatatag tagatggcgc aagacaaagc
     4921 gtcggaatag agatggctgc gcaggccccg caccagcaca aagccgcggc cataatccac
     4981 atcatggccc caggcccggg ccgcctcggc gcaggccacc agcggataat cggccgcctg
     5041 caccgtgcgc agatccgggc tttcttccac aaagcgcgcg cccagcgttt ccagctcctc
     5101 cagctgggcc gggttcagct catagatcca cacattggtg ccggccagct ggtcgccgcg
     5161 ccaggcgggc gcgccctcca ccgggcgcag gctgtcgatg accgtcatac ttctatcctt
     5221 tcagcgggaa atgggctggg tgcaatctag cccggccggc catggcattc ttgcccgcct
     5281 gccacgccag tttgtgcacc acgcgcacag gatggggttc aggccgcggc aaggctggcg
     5341 atataggccc gccagccgcc cagatgggtg atctcgctgg ccccttccag cgcccgcggc
     5401 tcggcgacaa agcccttcac ttcctcgccc gaggccagtg tgaccgtgcc aatggccaaa
     5461 ggggcgggca catcgaccac aaagctgccg aaagcggcaa cgcccagttc atagacctca
     5521 acctcgatgg cagcgcctgt gccggtggcg ccggcatgaa ccagagcggg cttgggcggc
     5581 gtgctttcgg ccagggcata gaggcggtaa tcgggcgcgg tgtgggtgcg cgccaccagc
     5641 ccggcctcgc gcgaggtcag ttgccagtgc agcggcatgc cggccagatg cgcgcccacc
     5701 acggccagct tcacggtctg cggcacgcct gccagatcgg gcgcggggat ctcgggcatg
     5761 ggggcaatct cctcataggc aagggccaga tcgacaagcg cccggtcggc aaaggccggg
     5821 ccgatgaaag tgacgccaaa gccggtggca ttggcgcgaa aaccggccgg cacggcaatc
     5881 gcggccatat ccagcaggtt cacgaaattg gtgtagagcc ccatgttggc gttgagcgcg
     5941 aagggctcgg ccagcatttc ggcgaggcga taggtggtgg ggcaagtggg cagcagcagc
     6001 gcgtccaccg cctcgaagat cggctctgcc tggcgggcca gcgccttcag cgcatattcg
     6061 ccccggaagg cctccacagc gctcatcgcc tgcccagcgc gcacgatatc gcgcaccacc
     6121 gggtggatat gctgcggccc atcgcgcagc agatcggcaa gggcggcggt gcgctcggcc
     6181 acccaggggc cctgatagag caggcgggcc gcttccatca gtggggtgat gtccacctcc
     6241 accagccggg cgccggtggc ggccacccgg gcgatcgccg cttcatagag cgcggccgag
     6301 gcgccatcgc ccagaaactg gcgctggtgg gccagcggca ggccgatacg cgccgctttg
     6361 gccgcaaagg ctggcgggct ggcaggctgg cggcgggcat agggatcggc ggcatcgaag
     6421 ccggcaaggc aggcgtccac cagcgcggca tcggccacat catgggcaaa aacggtgatg
     6481 caatcgaggc tgcggcaggc cggcaccagc ccgctgctgc tccagcggcc tttggtcggc
     6541 ttcagcccca ccagcccgtt gagcgcggcc ggcacccggc ccgatccggc cgtgtcggtg
     6601 cccagcgcaa aggccgccag ccccgccgcc accgccaccg ccgagcccga ggaagagccg
     6661 cccgagatat aggcgcggtt atgcaccgcc cggcacggcc catagggcga gcgcgtgccg
     6721 acaagcccgg tggcgaactg gtcgaggttg accttgccca gcggcagggc gccggcggca
     6781 atggcgcggg ccaccacatg ggcgctgtct tccggccgat aggcaaaggc ggggcagccg
     6841 gcggtggtgg caaggccggc aaggtcaata ttgtccttga tggcaaaggg cacgccggcc
     6901 agcggcaagt cctcgccggc ggccacccgc gcatcgatgc cgcgggcgat ggccagcagg
     6961 cgctcctcgt cgaagcggct gatccacagt tcgggctgga tgcgctcata ggcggcgatg
     7021 cgggccagca tggcgcgcgc cacgccctcg gccgtccatg cgccacgggc cacttgcggg
     7081 gcgatatcgg caatgcgggg caaggcgggc agctgcatgc gggtctccgg cggggaaggg
     7141 atcatcggcc cagcgcctgc ttgcgatagg cgcgcggcga aaggccatat tgcgcgcgaa
     7201 aggcgcggct gaaatgcgag gagccattga agccccagtt gaacgagatc tgcgagatgg
     7261 aaaggccggc atagatcggg ctggccagtt ccagccggca gcgctccagc cggcgcaggc
     7321 gcaggtagaa ggtgaaattc agcccctctg cggcaaacag cttctgcaga tagcggggcg
     7381 agacgccgct ttcctcggcc acttgcgcca gcgtcagctc cggctcgccc agcagcgcct
     7441 cgatcgcctg gcgaatgcgg tgaagctggg ccttggcgcg gctgcgcccg atatccagcg
     7501 ccggcgaggc cctgtcggcc agataggcga cgagaaattc cgccagcgcc acctcgatgg
     7561 gccgcaactg atcactgccc agcgcatcca gcccgccggc caccgctcgc agcagtgccg
     7621 aatagatggc gcccagccct tcatcgcccg ccaccagccc gatccccagc tgccgcgcgg
     7681 caacgatgcg atgggtgagc gccacctgcg ggatcgagat gaacagcagc cggaacgcgc
     7741 tggaaagccg cagcgccgca tcaacgccgg tgggcccata ggcgatatcg cccgggcgca
     7801 tggggcgcag gccatcgcca tcgtccagct ctgccgtgcc ttccagcagc agcgtcagcc
     7861 agatggcggc gggctggtgc gggttgcgcc cggcgatctc ctgcgcgctg cccgacagca
     7921 gcgccagctc catccccatc ggcgtggtgc ggcagatcac gctggcgtgg aaatccgcgc
     7981 cctctgccac cacgccctgc ggcaagcgca ggcgctccat cgtgcgggcc caggccttgc
     8041 ggcgctgggc gggggcatat tcatcgctgg agaagcgcca ggcgcgctcg cgcggctctt
     8101 cctgagcggc gggccatggc gcggcggggc tgaggctggc catggcccgc cgcgcccttc
     8161 gctcaggcgt ccaccggctg gcgcagggct tgccaggccc ggcgatagcg gcgcaccagc
     8221 cagtggttga actgcccctg cgcgccttcc tgccaggaat agcgcccgcg cacggcaaag
     8281 cgcgagcgca ggcccacctg caccagctcg tccacatgca tgtcctgggc gatgatcttg
     8341 gccgcgctgt tgacgatggc ttccagccgg tgctcgaagc cggggtcctt ctgggcgccg
     8401 ggggccacca gaatgccggt gtcctgcgcc atctgctcgg gcccgtcggc gcgcaggatg
     8461 aggtagatca ccatgtcggg cgtcatcacc agcgacaggg tgggcggcac attggcgaag
     8521 gttacccgcc cgcgctcctc atccgtcagc cccggaaaca ccggcaggat cgccttctgg
     8581 gtggggttga agctgggatc ctggtgcagc gcgccgttca gccgcaggaa gccggcatca
     8641 tcggggtctg cctcggggaa gctggccaga tggctgggga tgaaatcatg cagcggcccc
     8701 tggtgcagct tggaggcatg atagccatca ttgttgttct cgaacatcac cttccagttc
     8761 caggcgaaat cgccggtgaa cttctgggcc agctcggccg acggcaggtc gtagccggcg
     8821 atgtggcttt cgatggtggc gagccgcggc gccagcgggg cagcctctgc atcgaagttc
     8881 acgaacacga agccctgcca gatctccacc ttcagctcgg gcaggttgaa gctctgcttg
     8941 tcgaaattgc aggtgcggtc catcgccggg gcgcccacca gcttgccatc cagatcgtag
     9001 gtccagtggt gataggggca cacgaagccg cgggtgttgc cctcgccctc ggccaccagc
     9061 atggcgcggt gctggcacac cgagctgagc gcgcgcacct ggccggcgcg atcacgcacg
     9121 atcaccagcg gctcgcccac gatgctggtg gtgaaatagt cgccagcggc cttcacctgc
     9181 tcctcgcggc ccacgcacag ccattcgtgg ttgaacagcg cttccttctc gaattcgtag
     9241 aaggcggcat cggtgtagca ggcaggcggc agcgtctcgg cctcttccag cgcggcaaca
     9301 gaggtttcga gcgcggcgaa gaagcggtcg tcaagaaagg aagcggtcat gcggggcacc
     9361 ttgcagatga gcgccggatg cggcggggat ggagggcaat gtgccttggg gcgccgcgcc
     9421 cgtcttgcct gtgcgcgcac agactatggt gtggagcgcc tttttgcccc ccggcacgcc
     9481 gcgccgcacg ggcacgcatg cgcccacagt caacaatttg cgccgccgcc ctgcttgacg
     9541 gcaaccggcc ccggcctgtt catggcgcct ctttcttccc cttcgcaaac aggagcgcgc
     9601 catgctggtg aatggcaaat ggatcgagaa ctggcagccc gtgcagggca ccgatggcaa
     9661 gggcggcttc gtgcggcaaa gcgcgggctt tcgccactgg gtcacgcccg atggccgccc
     9721 cggccccagc ggcgagggcg gctttgcagc cagcgccggg cgctatcacc tctatgtcgc
     9781 gctcacctgc ccctgggcct cacgcgtgct gttcgcccgc cagctgaagg ggctgaccga
     9841 ggcgattggc gtcaccgtgg tggagccggt gctgaccgaa cagggctggc gctttggcga
     9901 ttatcccggc gccgaccgcg acacgctgaa cggcgccacc tacatgcacg agctctatac
     9961 cctggccgac cctgcctaca ccggccgcgc caccgtgccg gtgctgtggg acacagagcg
    10021 gcgcaccatc gtcaacaacg agagttcgga actgctgcgc atcctcaaca ccggctttgg
    10081 cgatctcgca agcgatgagg tggatctcta ccccgaggcg ctgcgcactg cgatcgacgc
    10141 gctgaacgag gagatctatc cgcggctcaa caatggcgtc tatcgcgccg gctttgccac
    10201 cacgcaggaa gcctatgagg aagccttcga cgatgtgttc ggcatgctgg acgcgctgga
    10261 ggcaaggctg gcggccggcg gcccctatct gctgggcgcg gcgctgaccg aggccgatat
    10321 ccgcctcttc gtcacgctgg tgcgctttga tgcggcctat cacggcatct tcaaatgcaa
    10381 cctcaggcgg cttgccgatt atcccgcgct cagcgcctat atgctgcggc tgctggcgct
    10441 gccgggcgcg cgcgccacgg tgaacatcga ccatatcaag cagggctatt attcgatcag
    10501 ggcgctcaac cccaatggca tcgtgcccaa ggggccggcg ctggcagggt tttaggcagg
    10561 caacaaaaag gggggcgagg ggccatcacg ccccccgccc ccctttttgc cctggcccgc
    10621 aagcctcagg cggaaacctc gctgccccac acatcgcggg cggtttcgaa cacgccctgg
    10681 tcgaaggaga attccagcgt gttgccgtcc gggtccttca ggaagcagat atagccgata
    10741 tccttgggca tctgcagcgg ccccagcgcc aggcagccgc cttccctgcc cttggcggca
    10801 atggcatcca ccgcttcctt gctgggcagc tcgatgccga tatgggcaaa cggccccagc
    10861 ggcggatggg cggccggcgc aaagggatcg tgcccttcat agaactggcc catcaccagc
    10921 acgaaggggc ggtccttctg gtcaggatcg gccagccaca cattcttgcc gtccttgtcg
    10981 gcatggcgag ccagcacgcg cagatgggtg aaggcttcat accagctggc agtggcatcc
    11041 acatcgcgca cgcacagcgc catatgggtc catgtcgggc gcttggggcc aatggagtgg
    11101 ttcacgtcgg tcatcttggc ggcctccctc tcgatcaggt cttgtcccaa actagcagcg
    11161 caaacgcccc aaggcttgat cgggcgcgtc cctttcattt gcccggaggc gcccagcccc
    11221 ccttttgcac cccttttttc accgccccgc agcagccatt ggccggggcc ttgcaccgat
    11281 cgccccctgc catgccgcct gcccggcgct gctggcccgc cccgcgtcaa tttcttgttc
    11341 gcagttgcac aagaccccgc cgccgccttt tccggatagt cgggccaacg ccgcacacca
    11401 cggcgcatga gggaaattca aatcataaaa gacaggtggc agacatgaaa aacgataatc
    11461 tgtgtacctc tatctcacac aaacgcggca tttcctatct ggcgatgtgc gccgcagccc
    11521 tggccggcgc cgcgccggca atggccgctg ccagcaccgc cgccaacacc gctgatgccg
    11581 ccgccagcac cgccgaggcc gctcctgaca gccagggcgc caggcagggc ctgggcgaga
    11641 tcaccgtgac cgccgagcgc cgcgtcatca gcctgcagaa ggcccccatc gccatcagcg
    11701 cgatcaacgc cgacagcctg aaggccagca atatcgtcga cgtaaccggg ctgaacggca
    11761 gcgtgccggg cctggtggtg gccaacagcg gcggcggcga gaagatgatc tcgatccgcg
    11821 gcatcggctc gcaaacgcct gagaacaccg acacccagcc cggcgtttcc tatcacgtcg
    11881 atggcgtcta catcttcaac tccatcgccg ccaatgccgc cttcatcgat gtggcgcagg
    11941 tggaagtgct gcgcggcccc cagggcacga tgttcgggca aggctccacc ggcggcacga
    12001 tcaacgtcgt ctccaaccag ccgaagctgg gcaagttctc cggcgagggc gagatcggcg
    12061 gcggcaatta cggctatttc atgggcaagg gcgccatcaa cgtgccgatc ggcgacacca
    12121 tcgccatccg cgccgcgatc gaccatgtgg gccacgaggg ctattccaag gtttcgagcg
    12181 ttgccggcaa tgccaatttc cgcgaggatg atgccaacaa caccggctgg cgcgtgtctg
    12241 ccctgtggca gccggcgccc aatttctcgc tcaccttcaa caccatccag tatcgcagcc
    12301 tgacccacgg gcaggcgatg aagaacgcgc tcgatcccaa ttccgacccg cgcgaggtga
    12361 cgcaggatta ccccggccgc acctatatca gcaccgccct ctattcgctg accgccaagt
    12421 gggacacgcc cttcgccacc gtccgctcga tcaccagcta tcagaacatg cacagcgaac
    12481 tgtcgtggga tgccgacggg ctgacccctt cgctgttcta tgccgagacc tatagcccgg
    12541 ccacctatgt cggctacaat tacgaccatg tggccctgtg gcagagcgat gtgaactccg
    12601 tcacccagga agtgaacctc acctcgaaca ggaagggccc gctcgaatgg atcgtcggcg
    12661 gggtctatct ccattccaag aacacccagt atatcaacga atacaaggcg gatgacagca
    12721 acccgctgcg cccggcgctg gccgaaagca ccgcttataa cgatgccgcc gtctcggccc
    12781 tcacctatgc cgatctggcc gagatcaccc gcgaggaaga agcggcctat ttccagctga
    12841 cctggaatgc caccgacaag ctcaccttca tcggcggcgc ccgcttcaac cacgaccgct
    12901 acaccggcgc tgccgacagc ttctcgaacg gcacccccga tgtaacctcg ggcgcctatc
    12961 tgcagatgaa gcccaccaag ggctatgcca ccagcgtgtg gaccggcaag gccgccgtgc
    13021 aatacaagct gacccccacc aacatggtct atttcagcta tacccgcggc ttcaagccgg
    13081 gcggcatcaa cgccaccgcc tctgccggcg ggtctgacct gatcgatgcc aactacaagc
    13141 cggaaatcgt cgattcgctg gaaatcggct cgaagaaccg cttcatgggc gatacgctgc
    13201 agttcaacgc ctctggcttc tattatttct acaaggacat gcagttcctc gattccgatc
    13261 cgcagcttta tggctatggc atctcgaacg tggggctggc gcatatctac gggctggagc
    13321 tggaaaccgc ctggctgccc agcaagcact ggaagctgga gggttcggcc tcgttcctgc
    13381 atggcgaggt ggcctcgcac accaccgggc tggaagccgg ccaggcgctg gcggcccagg
    13441 atgcggccgg ctacaccggc tatctctact ggtacaacac cactgccgcc gccgccgccc
    13501 gcgccggcgc cgagcaggat ctttacggca agaaggtgcc cggcatgccc agcctgcagg
    13561 gcaatatcgc gctcagctac atgaaccggg tgggcccggg cgatctgacc gcgcgcatcc
    13621 agtatctcta tcgcggcggc tatcaggcgc gcgtgttcaa cactgccttc gatgcggtgc
    13681 cggcctatga tcaggtgaac ctgatgctgc actacaagcc cgatcacctg ccgatcgaca
    13741 ttaccctgcg cgtgaccaac ctgttcaacg aggccggcgt caactcgcgc tacaccgacc
    13801 cctatggcag cgcgcaaagc ttcaccacct atatcccgcc gcgccagttc atcggctcgg
    13861 tcggcttccg gttctgagcc ttttttccgg acagccgacc ttccggcgg
//
LOCUS       DASRYE010000258        18439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_227478,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000258 DASRYE010000000
VERSION     DASRYE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 18439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18439
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227478"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFF98_13285"
     CDS             complement(<1..920)
                     /locus_tag="VFF98_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64649.1"
                     /translation="MSTEFVDIAIIGAGPGGLALAQGLKKHGIEVAVFEKDAVRADYV
                     QGFRLRMHQRGLDALQANLPPHLFAAFEATTGLAPATSLTVDEALHPLADTGPARDQA
                     HIDKSVSRITLRQVLLGGLDDIVHYGKRFAGYEVEPEGTVLVRFADGTRLRTRLLVGA
                     DGAGSAVRGQLLPAYRLVDTGKRRLAGKLSLAAAEKYGIPALLLDNNVAVRPRHGGRG
                     MMITAHRVDPAAFARFGLIGQDDPGHAGHQGLHFDNTASYVWWNTAYEEGELGSDAAL
                     EQLDGAGLIEALLGRIGHWHEALVKLVRHS"
     gene            complement(1069..2133)
                     /locus_tag="VFF98_13290"
     CDS             complement(1069..2133)
                     /locus_tag="VFF98_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU64650.1"
                     /translation="MVDYRYLGRSGLQVSPLTLGTMMFGGETDEPTAHRIIDTAREQG
                     INFIDTADGYNKGASEEVVGRGIKAHRDHWILATKFANPRGPGPNQRGQSRKWIIESV
                     ENSLRRLGTDHIDILYFHRAIFDAPLGEAVRAIADLIRAGKLRYFGVSNFRAWRLAEV
                     AHLADQLNIDRPIASQPLYNIVNRVAEAEQLPAASFYGLGVVSYSPLARGVLTGKYHP
                     GVAPGADTRAGRADKRILETEWRPESVALAAQIAEHARARGIDPVAFAIAWVLNNRLV
                     TSTITGPRTEAHWASYIKALDVQLTAEDEAFIDGLVTTGHASTHGFNDPSHPIEGRVP
                     RSHNAPAPLAVERSVRELVA"
     gene            complement(2153..3091)
                     /locus_tag="VFF98_13295"
     CDS             complement(2153..3091)
                     /locus_tag="VFF98_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZU64651.1"
                     /translation="MSLLAINDLSTHFRLPDGALRALDGVSLHVDAGETLGIVGESGC
                     GKSTLGKTILRLVEPTAGAIAFAGDDITALPARRLAAYRRRAQMVFQDPFASLNPRHT
                     IRKILTDPLIVHGIGGLKERRLRVEQILARVGLGPEALDRYPHEFSGGQRQRIGIARA
                     LILEPDLVICDEPVSALDLSIQAQTLNLLARMKADLGLSYIFISHDLSVVRYFTDRVV
                     VMYLGRIVEEAPTADLWARPQHPYTRALMASVPDPAQGRRALPLKGDLPSPQDIPEGC
                     RFHPRCPLATDLCRSSDPALRVAGSDAAHTVACHHA"
     gene            complement(3088..4116)
                     /locus_tag="VFF98_13300"
     CDS             complement(3088..4116)
                     /locus_tag="VFF98_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU64652.1"
                     /translation="MSAPLLSSAAATLARPRSIAPLLEVANLRIAFGRQEAVQGVSFT
                     LHEGETLALVGESGSGKSLTALSTLDLLPDGAAISGGTIAFAGADLAGLAPAARRALR
                     GGDIGMIFQEPMTSLNPVMTIGQQIIEALRQHQRLSRRAARTRALELLDLVRIADPHR
                     RIEDYPHQLSGGMRQRVMIAMAVAAGPRLLIADEPTTALDVTIQAQVLELIDRLRRDL
                     SMAVLLITHDLGVVGQWADRVVVMYAGRTVEEGDPAALFARPLHPYTQGLLAASPRLE
                     DSHHYTSGPLVEIPGSIASASGQPGCAFAPRCPQAAAACRIAPPPLRQWAAGRQVACP
                     RVLPEDLP"
     gene            complement(4113..5069)
                     /locus_tag="VFF98_13305"
     CDS             complement(4113..5069)
                     /locus_tag="VFF98_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64653.1"
                     /translation="MPPEPAAAALTPGTPAAPAVAAPQLAPVAAPAPLPAAPAANGLL
                     ATLRRHPPALIGAGVLLAIAVLALAAPLLFPGDPLAITGAPFLWPGTSLAHPLGTDSL
                     GRDILAGLAHGARVSLGIGFAAAGLGLVVGIALGALAGYLGGWVDAVLSQLIEIFQTL
                     PSFVLLVVLVSIAQPTTATLSGAIALVTWPMVARLARAECRSLREKDFVLAARSLGYG
                     HLRILVHEILPGALPPIIVTASVLVASAILMESALSFMGLGDPNTVSWGSMIGQGREY
                     LRSAWYMSALPGLAITLTVLALNLLGDALNEALNPRRKGTRR"
     gene            complement(5059..6057)
                     /locus_tag="VFF98_13310"
     CDS             complement(5059..6057)
                     /locus_tag="VFF98_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64654.1"
                     /translation="MKRHLPRLARIAGNTALRALPTAVGIVILGFFLLQLAPGDAADV
                     WAGEAGGAPAQATAAMRHHFGLDRPLGAQLAAYLSGLAHGDLGWSARFDVPVTHLILE
                     RLPGSLLLSATALVVALVAGVVLGTLMAAHEGRLLDRALSVVALLLYSLPSFWIALML
                     IVVFSIRLGWLPSGGAHTIGLPESGLAGLADRLRYMVLPVLSLALFYVAIYARLVRVG
                     VIEAKAQDYVRTARAKGLSERRILVRHVLRNALLPLTTVAGMHVGAMLGGAVVVETIY
                     GWPGLGRLAFEAVMGRDFRVLLGILLLSSLLVILANALVDILHGVLDPRVGASHAA"
     gene            6283..6807
                     /locus_tag="VFF98_13315"
     CDS             6283..6807
                     /locus_tag="VFF98_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU64655.1"
                     /translation="MLSQQSRYALKALIYLAAKDPSKPSPSAEIAAGANVPRKFLEAI
                     LLDLKLAGILHSTRGKAGGYTLARGPGEITFGDVIRVTDGPLALIHCASQKFYRRCLD
                     CVDEQACSLRRIMLEARAQLSAVLDHRTLADAIAGGDGLGQGLGLAGEAAPTGGPEAG
                     VQDASDSTRHAGRG"
     gene            complement(6875..7531)
                     /locus_tag="VFF98_13320"
     CDS             complement(6875..7531)
                     /locus_tag="VFF98_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZU64656.1"
                     /translation="MPHTIALLGAAGRIGSEIREEALRRGHQVTALVRHPGRLPPAPG
                     LTESVVDAYDAAALATAIAGHDALVSAFSPDPAEPFETKPARLREAHKAILDGARRAG
                     VARVILVGGVGSLWAAPGLRVVDSAEYGTANRGPTLANIEILQGLIDAGGDLEWSYVS
                     PPRRIEAGARTGLFRLGSDDLLRDADGESRISRADFAIAVIDELEQRAHIRQRFTAAY
                     "
     gene            7782..8852
                     /locus_tag="VFF98_13325"
     CDS             7782..8852
                     /locus_tag="VFF98_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZU64657.1"
                     /translation="MRLLEPFTLTNLALPNRAVMASLTRRRAGPGHLATPLMGEYYAQ
                     RAGAGLILSERIEVDPALAGNGQAGAPTAPALFNLAQARAWRAVVAQVHAAGGRIFAQ
                     LSHAGRISHPALRGGLAPVAPSARAAAGRIFTPAGEAPLPVPRALTLAEIAGLVEQFA
                     EAAAYARGAGFDGVEIDAAHGLLIDQFLRSGSNLRSDAYGGSARARVRFLLEVTEAVA
                     DIWGVGRVGVRLSPWSGCHGMRDALPRDTFTHAAAALDQLGVAFLHLVEQPGRRPSAL
                     ASLRGHFGGAVIAAGGYTRESAEAVLATGLADCVAFGSSFLANPDLPERFRRSAPLNP
                     PDPATFLAGGARGYTDYPSLHS"
     gene            8862..10466
                     /locus_tag="VFF98_13330"
     CDS             8862..10466
                     /locus_tag="VFF98_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64658.1"
                     /translation="MFALSRRDALLAGGLAGLLGPGLAGCAPAGGDGARGTLVFGTNP
                     EPPILTSAGTSAGPTQIVSTKIFDGLVTFDGDGRPQPQLATAWEWAADHLALTLRLRP
                     GVKWHDGQPFTSRDVAYSLEEVWKVFHSRGRSTFATVEKVETPDPLTAVLRLSTPAPY
                     LVSALGSAESQVIPAHLYAGRDFLSNPANAAPVGTGPFKFAEWQRGEFIRLERNPAYW
                     DAGKPGLDGLVFKFYSDAGAAAAALETGEIHLSTSTLISLDDLARLRRRPGFSVSARQ
                     SAFAATLSVFEFNLDRPPLRDPRVRQAFAHAIDKRFLLDNAWRGFGTVEDSPLTSALA
                     DFYDPASGHYPHDPARAIALLDAAGLRPDGQGVRLRLNHDPAPTGGQLLKSAEAVRDQ
                     LARVGVQLRIRTGDFASFIKRVYTDRDFDTVTYLAGVGPDPAIAVQRFYWSKNREKGV
                     AFSNGAGYASPEVDRLLESAQREVDPARRRADYVAFQQVVARDLPRIPLIGSANLLVA
                     GPRARGLPDTAYGMLDNFAGVSLAPA"
     gene            10518..11567
                     /locus_tag="VFF98_13335"
     CDS             10518..11567
                     /locus_tag="VFF98_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64659.1"
                     /translation="MTTPTTIWYTRCPVPTTSGIAQHFGWLQGEFARHGIAVESIRAA
                     ADKTVRLSHFDHTHENQFREGGNVPPLWARGQGRDTVVVGITWVDEEQLILVRDDSPL
                     HTAADLKGARLGLPLHDSRIVDIGRAEDLRGLLTALQIAGIARDEVSFVDVLGGDFDL
                     GENREVGGGDKLRLGAEALLAGKVDAIYAKGAFSATLIERHGFRPILDINEQADPLLR
                     VNAGTPRPITVNRALAAQHPEIVARYLAVLQQTAQWAATHPDAVVSAIAAETGATEAA
                     VRRGFGPHLHERFTVTLSDISVAGLRAQKDFLVAEGFITDFDFDSWIDPAPLALAATL
                     VSELRLADDSVSEPA"
     gene            11737..13005
                     /locus_tag="VFF98_13340"
     CDS             11737..13005
                     /locus_tag="VFF98_13340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64660.1"
                     /translation="MLFPLSLFVVRRPAGEAYAAFASRDFRLFFLARLINGFGTNIMI
                     PALGWQIYALTRSPLALGLIGLAVFVPVMLASIPAGQLADRFERRATYQAAQLVLVVT
                     AGLFMALSLAGVRAAWPFYLGAALFGAAKTLSMPVATAWMPHLIDRAHFPRAVSWTSF
                     TYQLSNTLGPVLAGWALYAGGEALIHGLAAGCYGVSLLLARAIATRSKGAAAARQGLA
                     GLFGGLAYICRNRLILAAVSLDVVALLLGGATALLPAYAYDVLHVGGRGFGLLRAAPA
                     LGALVTGLWLAHHPLRRRAGRWLLAAVATYGVAVAVFGLSRDMRLSLLALVVLGATEM
                     TGGFIRQTLIQLSTHDDMRGRVTAVNMMFVSARNELGEMESGFLASLVGVVPAVVAGG
                     LATVAVAGLWARLFPALRQVDRLSDAIAGQ"
     gene            13098..14216
                     /locus_tag="VFF98_13345"
     CDS             13098..14216
                     /locus_tag="VFF98_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU64661.1"
                     /translation="MTQPQTQPHQISAATFAAWLDDGAELAVVDIRSPQDIVYYGSPL
                     FGKNVPAATLLDEIGLYVPRKAVRTVLVDAGDGQAETFAAQLRARGWPHLVALEGGFP
                     GWLESGLADRSFDIPGKTFALAVRDELGTPVTTVEQLAEQRARGEDVIVIDTRTVPEY
                     TRDHVPGAVNIPGAELLLRLPELVPSASTQVVVSCAGLPRAILGAQTLIDAGVANPVS
                     YLDDGTAAWRRAGWELETGATRVYGAETAEGRAFGRSHAAHLPTAGRFPEVDLATARA
                     WAADPERTTYLLDVRTPEEYAEGHLEGTLSAQGGQLLGLASRTIATRGARLVLIDDED
                     GIRAATVAHWLSRRNFEIAILRHAFAHAGAGAETTAQA"
     gene            complement(14296..15555)
                     /locus_tag="VFF98_13350"
     CDS             complement(14296..15555)
                     /locus_tag="VFF98_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64662.1"
                     /translation="MSDTITLAPAPRQRPAAPPALSREAILAGLPEVLARVAEGAAER
                     ERARHLPHELFAWLRTTGLTWLRVPESLGGPGGTLADQVEVTVALASADSNVAHALRS
                     HFGFLEGLATDPTSARSRAHAPQVLAGRLFGGAHMEIGTPRPNQLRTKLTREGDHFRL
                     NGTKYYATGAIFADFESFSAVGEDGQITHVLIPADRPGLTLLDDWDGMGQTLSASGTV
                     LLDNVEVRPGEVAERNGHNPFRQRHGATRAQLHLVAVMGGILRNVLADAISYTTTRAR
                     SAKHSSSATAAGDPFVQQTVGDLSALVHITDLAIADVARRLDLTAAALARGESDPAVL
                     DALVLNAQLANSKAQVMVGRLAQEGAQRLFDTGGGSATSITLAFDRHWRNVRTLLNHN
                     PALLRGRVLGDFYLNGERADFDEGRVF"
     gene            complement(15665..16861)
                     /locus_tag="VFF98_13355"
     CDS             complement(15665..16861)
                     /locus_tag="VFF98_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZU64663.1"
                     /translation="MRLLALIGALLALLGAGMASAHTRSESHSSWLIDGRFARASFTL
                     AAPEARRLTADGRLPTGAAFLAYVAAHLTLADHGQPCASAAPPRMVGAAAGYLRAELA
                     FACADGKALAITSTVLADKVPGHLHFAQIHRTDGAFAEDVLSTDQPTAAIAAAGQGPL
                     ATAGLFAFIGMGMMHIFTGVDHMSFLVGLVLLSRRLRDLLFVVTGFTLGHSLTLALAV
                     TGLIRPHAEYIDALVALTIALIGAENASAAAGRPATVALAAAGGLLAMAGLRLAGIGT
                     LPPLLLAGMGLFAPSYILLSAQLADAGRVRLVVTLVFGLIHGFGFAADLLENRLPAAR
                     LAEILLGFNLGVELAQLAIVSALVTGTAWLVRHGRALPRALVADAGAAGLVGLGTFWF
                     IARSFA"
     gene            complement(16858..17742)
                     /locus_tag="VFF98_13360"
     CDS             complement(16858..17742)
                     /locus_tag="VFF98_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZU64664.1"
                     /translation="MRPLPERLRPGPARTLWLCAAGAVAGLLLAGLGLFTAHGTRIAG
                     VLPEDVALVNQVPILMADYVDQLRARFGVGLAAATPAQRRQVLDEMIREELYVQRGVE
                     LGLPADTLEVRQALVGAVEAQEAADATLSAPDEAALRADYAAHAADYADEGVIALDEY
                     RLPASQAPRARAAAQALRAAHGAPQVAAALGLAATGRMADGAEFYFAARAHLGASLFA
                     EAARLPAGAVSDPLPLPDGLHILAVHANTPPRPAPFALVRDRVLADATARQTRALIGA
                     ADRFLRARADIAIRPGYE"
     gene            complement(17726..>18439)
                     /locus_tag="VFF98_13365"
     CDS             complement(17726..>18439)
                     /locus_tag="VFF98_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU64665.1"
                     /translation="GLMALGTGAHALAAWQGARQLVAADPDRILATPALRATAIAQGG
                     PLFARHCAACHNADGRGRPDLGAPDLTDADWLYGSGRVDQIEQIIRHGIRAGDTRGQA
                     LADMPAFGRARPSASEPFVPLGPGEIALLVDWLHYLEGRAPLTGPARAGGQIYAGKGG
                     CWDCHGADGHGDPAIGAPDLADRVWLYGDGSAGAIARSITYGRRGVMPAFARRLSAAE
                     TRALAAYVATLASPHAPAA"
BASE COUNT         2753 a         6297 c         6644 g         2745 t
ORIGIN      
        1 tcggaatggc gcaccagctt caccagcgcc tcgtgccaat ggccgatccg gcccagcagt
       61 gcctcgatca accccgcgcc atccagctgt tccagcgcgg catcgctgcc cagctcgccc
      121 tcttcatagg cggtgttcca ccacacatag ctggcggtat tgtcgaaatg gaggccctgg
      181 tggccggcat ggccggggtc gtcctggccg atgaggccga aacgggcgaa agcggccgga
      241 tcgaccctgt gcgcggtgat catcatgccg cgcccgccat ggcgcggccg caccgccaca
      301 ttgttgtcga gcagaagggc ggggatgccg tatttctcgg ccgcggccag gctcagcttg
      361 ccggcaaggc ggcgcttgcc ggtatcgacc agccgatagg ccggcagcag ctgcccgcgc
      421 acggcagagc cggcaccatc ggcccccacc agcagccgcg tgcgcagccg ggtgccatct
      481 gcaaagcgca cgagcacggt gccctcgggc tccacctcat agccggcgaa gcgcttgcca
      541 taatgcacga tatcgtccag cccgcccagc aacacctggc gcagggtgat gcggctgacc
      601 gatttgtcga tatgggcctg atcgcgcgcg ggcccggtgt cggccagcgg gtgcagggcc
      661 tcgtccaccg tcaggctggt ggcgggggca aggccggtgg tggcctcgaa ggcggcgaac
      721 aggtggggcg gcaggttggc ctgcagggca tccagccccc gctgatgcat gcgcaggcga
      781 aagccctgca cataatcggc gcgcaccgca tccttttcga acacggcgac ctcgatgccg
      841 tgtttcttca gcccctgcgc cagagccagc ccgccggggc cggcgccaat gatggcgata
      901 tcgacaaatt ctgtgctcat gatgtgtcac tcgctcggga tattgcggat ctggcgggcc
      961 cttatgggca gcgcctgttg tctttctccc catctgagtg ggtgtggccc ggtggctttt
     1021 gccagccgtg gccggggccg ggcgggaagg gccccgcccg gcgggccatc aggccaccag
     1081 ttcgcgcacg ctgcgttcca ccgccagcgg cgcaggcgcg ttgtggctgc ggggcacgcg
     1141 gccctcgatc gggtggctgg gatcgttgaa gccgtgggtg gaggcatggc cggtggtgac
     1201 caggccatcg atgaaggctt cgtcctcggc ggtcagctgc acatccagcg ccttgatgta
     1261 gctggcccag tgcgcctcgg tgcgcgggcc ggtgatggtg gaggtgacca gccggttgtt
     1321 gagcacccag gcgatggcaa aggctaccgg atcgatgccg cgggcgcggg cgtgctcggc
     1381 gatttgcgct gccagcgcca ccgattcggg ccgccattcg gtctcgagga tgcgcttgtc
     1441 ggcgcggccg gcgcgggtgt cggcaccggg ggccacgccg gggtggtatt tgccggtgag
     1501 cacgccgcgc gccagcgggc tgtaggacac cacgcccagg ccatagaagc tggcggcggg
     1561 cagctgctcg gcctcggcca cgcggttgac gatattgtag agcggctggc tggcgatcgg
     1621 ccggtcgatg ttcagctggt cggccagatg ggccacttcg gcaaggcgcc aggcgcgaaa
     1681 gttcgatacg ccgaaatagc gcagcttgcc cgcgcggata aggtcggcga tggcgcgcac
     1741 ggcctcgccc agcggggcat cgaaaatggc gcggtggaaa tagaggatgt cgatatggtc
     1801 ggtgcccagc cgcctgaggc tgttctcgac gctttcgatg atccacttgc gcgactggcc
     1861 gcgctggttg gggccgggcc cgcgcggatt ggcgaatttg gtggccagga tccagtgatc
     1921 gcgatgggcc ttgatgccgc ggcccaccac ttcctccgag gcgcccttgt tgtagccgtc
     1981 ggcggtgtcg atgaagttga tgccctgttc acgggcggtg tcgatgatgc ggtgggcggt
     2041 gggctcgtcg gtctcgccgc cgaacatcat cgtgcccagc gtgagcggcg agacctgaag
     2101 ccccgagcgg ccgagataac gatagtctac catggaatgc tccttgcgat gttcaggcgt
     2161 ggtggcaggc gacggtgtgg gccgcatcgc tgccggccac gcgcagggcc ggatcgctgc
     2221 tgcggcacag gtctgtggcc agcgggcagc gggggtggaa acggcagccc tctgggatgt
     2281 cctgcgggct gggcagatcg cccttcagcg gcagggcgcg ccggccctgg gcgggatcgg
     2341 gcacgctggc catcagcgcg cgggtatagg gatgctgcgg gcgggcccac agatcggccg
     2401 tgggcgcttc ctcgacgatg cggcccagat acatcaccac cacccgatcg gtgaaatagc
     2461 gcactaccga gagatcatgc gaaatgaaga tataggagag gccgagatcc gccttcatgc
     2521 gggccagcag gttcagcgtc tgcgcctgga tggacagatc gagcgcggag accggctcgt
     2581 cgcagatgac cagatcgggc tcgaggatca gcgcccgggc gatgccgatg cgctggcgct
     2641 ggccgccgga aaattcatgc ggatagcgat cgagcgcttc tggccccagc cccacccggg
     2701 ccagtatctg ctccacccgc aggcggcgct ccttcagccc gccaatgccg tggacgatga
     2761 gcggatcggt gaggatcttg cggatcgtgt ggcgcgggtt gagcgaggcg aacggatcct
     2821 ggaacaccat ctgcgcgcgg cggcgatagg cggccaggcg ccgggcgggc agggcggtga
     2881 tgtcgtctcc ggcaaaggcg atggcgcccg cggtgggctc caccagccgc aggatggttt
     2941 tgcccagggt tgatttgccg cagcccgatt cgcccacgat ccccagcgtc tcgcccgcat
     3001 cgacatgcag cgagacgcca tcgagcgcgc gcagggcgcc atcgggcagg cggaaatggg
     3061 tggacagatc gttgatcgca agcaggctca tggcaggtct tccggcagaa cgcgcgggca
     3121 ggccacctgc cggccggcgg cccactggcg cagcggcggc ggggcgatgc ggcaggccgc
     3181 cgctgcctgc gggcagcgcg gggcaaaggc gcagcccggc tggccgctgg cagaggcgat
     3241 cgagcccggt atctccacca gcgggcccga ggtgtagtgg tggctgtctt ccagccgggg
     3301 agaggcggcc agcagccctt gcgtgtaagg gtgcagcggg cgggcgaaga gagcggccgg
     3361 atcgccttcc tccaccgtgc gcccggcata catcaccacc acccgatcgg cccactggcc
     3421 caccacgccc agatcatggg tgatcagcag caccgccatg gaaagatcgc gccgcagccg
     3481 gtcgatcagt tccagcacct gcgcctggat cgtcacatcg agcgcggtgg tcggctcgtc
     3541 ggcgatcagc aggcgggggc cggcggccac ggccatggcg atcatcaccc gctggcgcat
     3601 gccgcccgac agctggtggg gataatcctc gatgcggcga tggggatcgg cgatgcgcac
     3661 cagatcgagc agttcgagcg cgcgggtgcg cgcagcccgg cgcgacagcc gctggtgctg
     3721 gcgcagcgcc tcgatgatct gctggccgat ggtcatcacc gggttgaggc tggtcatcgg
     3781 ctcctggaag atcatgccga tatcgccgcc gcgcagggcc cgccgcgccg ccggggcaag
     3841 gccggccaga tccgcgccgg caaaggcgat ggtgccgccg ctgatcgccg cgccatcggg
     3901 cagcaggtcc agggtggaca gcgcggtgag cgatttgccc gagcccgatt cgcccaccag
     3961 cgccagcgtt tcgccttcgt gcagcgtgaa gctcacgccc tgcacggctt cctgccggcc
     4021 aaaggcgatg cgcaggttgg ccacttccag cagcggggca atgctgcgcg ggcgggccag
     4081 cgtggccgcg gcggaagaga ggaggggggc gctcatcggc gggtgccctt gcggcgcggg
     4141 ttcagcgctt cgttcagcgc atcgcccagc aagttcagcg ccagcacggt gagggtgatg
     4201 gcaaggccgg gcagcgcgct catataccag gcgctgcgca gatattcgcg gccctggccg
     4261 atcatgctgc cccagctgac ggtgttggga tcgcccagcc ccatgaacga cagcgccgat
     4321 tccatgagaa tggccgaggc caccagcacc gaggccgtga cgatgatcgg cggcagggcg
     4381 cccggcagga tctcgtgcac gaggatgcgc agatggccat agcccaggct gcgggcggcc
     4441 agcacgaaat ccttctcgcg cagcgaacgg cattcggcgc gggccaggcg cgccaccatc
     4501 ggccaggtga ccagcgcgat ggcgcccgag agcgtggccg tggtgggctg ggcgatcgac
     4561 accagcacca ccagcagcac aaagctgggc agggtctgga agatctcgat gagctgcgag
     4621 agcacggcat ccacccagcc gcccagatag cccgccagcg cccccagcgc gatgcccacc
     4681 accagcccca ggcctgccgc cgcaaagcca atgcccagcg acacgcgggc gccatgggca
     4741 aggccggcca gaatgtcgcg gcccagcgaa tccgtgccca gcgggtgggc caggctggtg
     4801 cccggccaca ggaagggcgc gccggtgatc gccagcggat cgccggggaa cagcagcggc
     4861 gcggccagcg ccagcaccgc gatggccagc agcacgccgg cgccgatcag cgcgggcgga
     4921 tggcggcgca gcgtggccag caggccattg gcggcagggg cggccggcag gggcgccggg
     4981 gcggccaccg gggccagttg cggcgcggcc acggcgggcg cggcaggggt gcccggggtg
     5041 agggcggcgg cggcaggttc aggcggcatg gctggccccc acgcgcggat caagcacgcc
     5101 atgcaggata tcgaccagcg cattggccag gatcaccagc agcgaggaga gcagcaatat
     5161 gcccagcaac acgcgaaaat cgcggcccat caccgcctcg aaggccagcc ggcccaggcc
     5221 cggccagcca tagatcgtct cgaccaccac cgccccgccc agcatcgcgc cgacatgcat
     5281 gccggccacg gtcgtcagcg gcagcagggc gttgcgcagc acatggcgaa caaggatgcg
     5341 ccgttccgac aggcccttgg cgcgggcggt gcgcacataa tcctgcgcct tggcctcgat
     5401 cacccccacg cgcaccagcc gggcgtagat cgccacatag aacagcgcca gcgagagcac
     5461 cggcagcacc atgtagcgca gccggtcggc cagcccggcc agcccgcttt cgggcaggcc
     5521 gatggtgtgg gcgccgccgc tgggcagcca gcccaggcgg atggaaaaca ccacgatcag
     5581 catcagcgcg atccagaagg agggcagcga atagagcagc agcgccacta ccgacagcgc
     5641 ccggtcgagc aggcggcctt catgggcggc catcagcgtg cccagcacca cgcccgccac
     5701 cagcgccacc accagcgccg tggccgacag cagcagcgag ccgggcaggc gctcaaggat
     5761 gagatgggtg acgggcacgt cgaaccgggc cgaccagccc agatcgccat gcgccaggcc
     5821 cgacagatag gccgccagct gggcgcccag cggccggtcc aggccgaagt ggtggcgcat
     5881 ggcggcagtg gcctgcgccg gggcgccgcc ggcctcgccc gcccacacat cggccgcatc
     5941 gcccggcgcc agctgcagca ggaagaagcc gaggatgaca atgccgacgg ccgtgggcag
     6001 ggcgcgcagg gcggtgtttc cggcgatacg ggcaaggcgg ggcaggtggc gtttcatgtg
     6061 ggggtccggc tgagggcgag cggacagggg ctcgtcaggg gtttaatcct gattaaatca
     6121 atagagattg tcaatattga ggcggagccg ccgcccgaga agaaattata tggcaagccc
     6181 aaccgctggt tgaccggccc gcttgccccg cctggttggg atgcttgctt ttgccgggcg
     6241 atctgcctag aaagccgctc tttcggcaac aagggcagcc acatgctatc gcagcaatcg
     6301 cgctacgcat tgaaggcgct tatttatctt gccgccaagg atccttccaa gccctctccc
     6361 agcgccgaga tcgccgctgg cgccaatgtg ccgcgcaagt tcctcgaggc gatcctgctc
     6421 gatctcaagc tggccggcat tttgcacagc acgcgcggca aggcgggcgg ctacacgctg
     6481 gcgcgcggcc cgggcgagat cacctttggc gatgttatcc gcgtgacgga cgggccgctg
     6541 gcgctgatcc attgcgccag ccagaaattc tatcgccgct gcctcgattg cgtggacgag
     6601 caggcctgca gcctgcgccg gatcatgctg gaggcgcggg cgcaattgtc ggcggtgctc
     6661 gatcaccgca cgctggccga tgccatcgct ggcggtgatg gtttgggtca gggcctgggc
     6721 cttgccggag aagccgcgcc aaccggcggg ccggaagccg gcgtgcagga tgcgtcggac
     6781 agcacccggc acgccggccg gggctgaaca ccggctccct cccacaggac cggcggccac
     6841 ccccgaaact ctgaaatccc gcaagctgtc gccttcaata ggcggcggtg aagcgttggc
     6901 ggatgtgggc gcgctgttcc agctcgtcga tcacggcgat ggcgaaatcg gcgcgcgaga
     6961 tgcggctttc gccatcggca tcgcgcagca gatcatcgct gcccaggcgg aacaggcccg
     7021 tgcgcgcgcc cgcctcgatg cgccgcggcg gtgagacata gctccattcc aggtcgccgc
     7081 cggcatcgat cagcccttgc aggatctcga tattggccag cgtgggcccg cgattggcgg
     7141 tgccatattc cgcgctgtcg accaccctga ggccgggcgc ggcccacagg ctgcccaccc
     7201 cgccgaccag aatgacccgc gccacgccgg cccgccgcgc gccatcgaga atggccttgt
     7261 gcgcctcgcg caggcgggcg ggcttggttt cgaagggttc ggccggatcg gggctgaagg
     7321 ccgagacgag cgcgtcgtgc ccggcaatgg ccgtggccag cgcggcggca tcataggcat
     7381 ccaccacgct ttcggtcagc cccggggcgg gcggcaggcg gccaggatgg cgcaccagcg
     7441 cggtgacctg gtggccacgg cgcagcgctt cctcgcggat ttccgagccg atgcggccgg
     7501 ccgcgcccag cagggcgatg gtgtggggca tggataactc cgaatggggc gggctgcggg
     7561 aagggagcag ctggcgggca atgtatatcc tatcgtaaat ataggaatta agatttcgta
     7621 tggcggggcc aagcctgcct atcccgatgg gcgcctcgcc cggaaatatc gcttgggggt
     7681 ggacggcaaa gaaaaatata ttaatcccat aggaattatt ttttctgtat ccggcgctgc
     7741 ccgcttaagt ctggcctgcc cttcccccca gcgaggcatc catgcgtctg ctcgagccct
     7801 tcaccctcac gaatctggcc ttgcccaatc gcgcggtgat ggcctcgctc acccggcgcc
     7861 gggcagggcc gggccatctc gccaccccgc tgatgggcga atattatgcc cagcgggcgg
     7921 gcgccgggct tatcctgtcc gagcggatcg aggtggatcc ggcgctggcg ggcaacgggc
     7981 aggcgggcgc gcccacggcg ccggcgctgt tcaatctcgc tcaggcgcgc gcgtggcggg
     8041 cggtggtggc gcaggtgcat gcggcgggcg ggcgcatctt tgcccagctc tcgcatgccg
     8101 ggcgcatcag ccatccggcg ctgcgcggcg ggctggcgcc ggtggcgccc tcggcgcggg
     8161 cggcggcggg gcgcatcttc acgccggccg gcgaggcgcc gctgcccgtg ccgcgcgccc
     8221 tgacgctggc cgaaatcgcc gggctggtgg agcaattcgc cgaggccgcc gcctatgccc
     8281 ggggcgccgg cttcgacggg gtggagatcg atgccgccca cggcctgctg atcgaccagt
     8341 tcctgcgctc tggcagcaat ctgcgcagcg atgcctatgg cggatcggcc cgcgcccgcg
     8401 tgcgcttcct gctggaagtg accgaggcag tggccgacat ctggggcgtg ggccgggtgg
     8461 gggtgcgcct gtcgccgtgg agcgggtgcc atggcatgcg cgatgccctc ccgcgggaca
     8521 cgttcaccca tgccgccgcc gcgctcgacc agctgggcgt ggccttcctg catctggtgg
     8581 aacagccggg gcggcggcct tcggcgctgg cgagcctgcg cggccatttc ggcggcgcgg
     8641 tgatcgcggc cggcggttac acgcgcgaga gcgccgaggc ggtgctggcc accggccttg
     8701 ccgattgcgt ggcctttggc tccagcttcc tggccaaccc cgacctgccc gagcgttttc
     8761 gccgcagcgc cccgctgaac ccgccggatc cggccacttt cctggccggc ggcgcgcgcg
     8821 gctacaccga ttatccctcc ctgcattcct gaggttctga catgtttgca ctttcccgcc
     8881 gtgatgcgct gctggccggg ggccttgccg gcctgctggg gccggggctg gctggctgcg
     8941 cgccggccgg cggcgatggc gcccgcggca cgctggtgtt cggcaccaat cccgagccgc
     9001 cgatcctgac cagcgcgggc acttcggccg gccccaccca gatcgtctcc accaagatct
     9061 tcgacgggct cgtcactttc gatggcgatg gtaggccgca gccccagctg gccacggcct
     9121 gggaatgggc ggccgatcat ctggcgctga ccttgcggct gcgccccggc gtcaaatggc
     9181 acgatggcca gcccttcacc tcgcgcgatg tcgcctattc gctggaggag gtgtggaagg
     9241 tcttccacag ccggggccgt tccaccttcg ccacggtgga gaaggtggaa acgcccgatc
     9301 cgctgaccgc cgtgctgcgc ctttccacgc ccgcgcccta tctcgtctcg gcgctgggct
     9361 cggccgaatc gcaagtgatc ccggcgcatc tctatgccgg cagggatttc ctctcgaacc
     9421 cggcgaatgc cgcgcctgtg ggcaccggcc cgttcaaatt cgccgaatgg cagcggggcg
     9481 aattcatccg gctggagcgc aacccggcct attgggatgc cggcaagccg ggtctcgatg
     9541 gcctcgtctt caagttctac agcgatgcag gggcggccgc cgcggcgctg gaaaccggcg
     9601 agatccacct gtccaccagc acgctgatca gcctggatga tctggcccgc ctgcgccggc
     9661 ggccgggctt cagcgtttct gcccggcaga gcgcctttgc cgccacgctg tcggtcttcg
     9721 agttcaacct cgaccggccg cccttgcgcg atccgcgcgt gcgccaggcc tttgcccatg
     9781 cgatcgacaa gcgctttctg ctcgacaatg cctggcgcgg tttcggcacg gtggaggatt
     9841 cgccgctgac cagcgcgctg gctgatttct acgatcccgc ctcgggccat tatccgcatg
     9901 acccggccag ggcgatcgcg ctgctcgatg cggcggggct gaggcccgat gggcaggggg
     9961 tgcgcctgcg cctcaaccat gatccggcgc ccaccggtgg gcaattgctg aaatcggccg
    10021 aggcggtgcg cgatcagctg gcccgggtgg gggtgcagct gcgcatccgc accggcgatt
    10081 ttgccagctt catcaagcgc gtctacaccg accgcgattt cgatacggtg acctatctgg
    10141 ccggcgtcgg ccccgatccg gcgattgccg tgcagcgctt ctactggtcg aagaacagag
    10201 agaagggcgt cgccttctcc aatggcgctg gttatgccag ccccgaggtc gatcgcctgc
    10261 tcgaaagcgc ccagcgcgag gtggatccgg cccgacgccg cgccgattat gtcgccttcc
    10321 agcaggtggt ggcgcgcgat ctgccgcgca tcccgctgat cggcagcgcc aacctgctgg
    10381 tggccggccc gcgcgcccgc ggcctgcccg acaccgccta tggcatgctc gacaatttcg
    10441 ccggggtttc cctggccccg gcctgagccg gccccgcatc cctcccgcgc ccacatccct
    10501 tttgagagga gaccgccatg accaccccca ccaccatctg gtacacccgc tgcccggtgc
    10561 ccaccacttc gggcattgcc cagcattttg gctggctgca gggcgaattt gcccgccatg
    10621 gcattgccgt ggaatcaatc cgcgcggcgg ccgacaagac cgtgcgcctc tcccatttcg
    10681 accataccca cgaaaaccag ttccgcgagg gcggcaatgt gcccccgctg tgggcgcgcg
    10741 ggcaggggcg cgatacggtg gtggtgggca tcacctgggt ggatgaggag cagctgatcc
    10801 tcgtgcggga cgacagcccg ctgcacaccg ccgccgatct gaaaggcgcg cgcctgggcc
    10861 tgccgctgca cgacagccgg attgtcgata tcggccgggc ggaagatctg cgcgggctgc
    10921 tgacggcgct gcagatcgcc ggcatcgccc gcgacgaggt gagcttcgtc gatgtgctgg
    10981 gcggcgattt cgatctgggc gagaaccgcg aggtgggcgg cggcgacaag ctgcggctgg
    11041 gcgccgaggc gctgctggcg ggcaaggtgg atgcgatcta tgccaagggg gccttcagcg
    11101 ccacgctgat cgagcgccac ggcttccgcc ccatcctcga catcaacgaa caggccgatc
    11161 cgctgctgcg ggtgaatgcc ggcacccccc ggccgatcac cgtgaaccgc gcgctggccg
    11221 cccagcaccc cgaaatcgtc gcccgctatc tggccgtgct gcagcagacc gcgcaatggg
    11281 ccgccaccca tccggatgcg gtggtcagcg ccattgccgc ggaaaccggc gccaccgagg
    11341 cggccgtgcg ccgcggcttt ggcccccacc tgcacgagcg cttcaccgtt accctttccg
    11401 acatcagcgt ggcgggcctg cgcgcccaga aggatttcct ggtggcagaa ggcttcatca
    11461 ccgatttcga tttcgacagc tggatcgacc cggcgccgct ggccctggcc gccacgctgg
    11521 tcagcgagtt gcgcctggcc gacgacagcg tgagcgagcc ggcctgagga cccccacgtt
    11581 cttgtgatga tgcaggcaca ctggcgctgg tgagcctgct gccggggcgt cgcggcaggg
    11641 cagggaggct tgccgcggcg tttccgcttc tgatggggcg cgggcaggcc atggggcgcc
    11701 cgcgcctgtt gcatgccccc aggcaaggta gccggtgtgt tgtttcccct ttcgcttttt
    11761 gttgtccgcc ggccggcggg cgaggcctat gccgcctttg cctcgcggga tttccggctg
    11821 tttttcctgg cccggctgat caacggcttt ggcaccaaca tcatgatccc ggcgctgggc
    11881 tggcagatct atgcgctcac ccgctcgccg ctggcgctgg ggctgatcgg ccttgcggtg
    11941 ttcgtgccgg tgatgctggc ctcgatcccc gccggccagc tggctgacag gttcgagcgg
    12001 cgcgccacct atcaggccgc gcagctggtg ctggtggtca cggcggggct gttcatggcg
    12061 ctcagcctgg cgggggtgcg ggcggcctgg ccgttctatc tgggcgcggc gctgttcggc
    12121 gcggccaaga ccctgtcgat gccggtggcc accgcctgga tgccccacct gatcgaccgg
    12181 gcccatttcc cccgcgccgt cagctggacc tccttcacct atcagctttc caatacgctg
    12241 ggcccggtgc tggccggctg ggcgctttat gccggcggcg aggcgctgat ccacgggctg
    12301 gcggcgggct gctatggcgt gtcgctgttg ctggcgcggg ccattgccac gcgcagcaag
    12361 ggggcggcgg cggcccggca ggggctggcg gggctgtttg gcgggctggc ctatatctgc
    12421 cgcaaccggc tgattctggc ggcggtcagc ctggatgtgg tggcgctgct gctgggcggg
    12481 gccacggcgc tgctgccggc ctatgcctat gatgtgctgc atgtgggcgg gcgcggcttt
    12541 ggcctgctgc gcgccgcgcc tgcgctgggc gcgctggtca ccggcctgtg gctggcgcat
    12601 catccgctgc ggcggcgggc ggggcgctgg ctgctggcgg cggtggcgac ctatggcgtg
    12661 gcggtggcgg tgttcggcct ctcgcgcgat atgcgcctgt cgctgctggc gctggtggtg
    12721 ctgggcgcca ccgagatgac cggcggcttc atccgccaga cgctgatcca gctgtccacc
    12781 cacgacgaca tgcgcggccg ggtgacggcg gtgaacatga tgttcgtgag cgcgcgcaac
    12841 gagctgggcg agatggaatc gggctttctg gcctcgctgg tgggcgtggt gccggcggtg
    12901 gtggccggcg ggctggccac ggtggcggtg gcgggcctgt gggcgcgatt gttcccggcg
    12961 ctgcggcagg tggacaggct gagcgatgcg atcgccggcc aataaaaacc tacctactag
    13021 ataggaatta tgtctttggc gccgccgccg ccgcgctaga ctggcgcttc cacatcacca
    13081 gagaggagcg ccccccaatg acccagcccc agacccagcc ccaccagatc tccgccgcca
    13141 ctttcgccgc ctggctcgac gatggcgcgg agctggccgt tgtcgatatc cgttcgccgc
    13201 aggatatcgt ctattacggc agcccgcttt tcggcaagaa cgtgcccgcc gccaccctgc
    13261 tcgacgagat cggtctctat gtgccgcgca aggcggtgcg cacggtgctg gtcgatgccg
    13321 gcgatggcca ggccgaaacc tttgccgccc agctgcgcgc aaggggctgg ccgcatctgg
    13381 tggcgctgga aggcggcttt ccgggctggc tggaaagcgg cctggcggat cgctccttcg
    13441 atatccccgg caagaccttc gcccttgccg tgcgcgatga gctgggcacg ccggtcacca
    13501 ccgtcgagca gctggccgaa cagcgcgcgc gcggcgagga tgtcatcgtc atcgacaccc
    13561 gcaccgtgcc ggaatacacg cgcgaccatg tgcccggcgc ggtgaacatc ccgggcgccg
    13621 aactgctgct gcgcctgccc gaactggtgc cttcggccag cacgcaggtg gtggtgtcct
    13681 gcgctggcct gccgcgcgcg atcctgggcg cgcaaacgct gatcgatgcc ggcgttgcca
    13741 atccggtttc ctatctggac gatggcaccg ccgcctggcg ccgcgccggc tgggagctgg
    13801 agaccggcgc cacccgcgtc tatggcgccg aaaccgccga gggccgcgcc tttggccgca
    13861 gccatgccgc gcatctgccc accgctggcc gcttccccga agtggatctg gccaccgccc
    13921 gcgcctgggc ggcagaccct gagcgcacca cctacctgct cgatgtgcgc acgcccgagg
    13981 aatatgccga aggccatctg gaaggcacgc tgtcggccca gggcgggcag ctgctgggcc
    14041 ttgccagccg cactatcgcc acccgcggcg cccggctggt gctgatcgac gatgaggatg
    14101 gcattcgcgc cgccacggtc gcccattggc tgtcgcgccg caacttcgag atcgccatcc
    14161 tgcgccatgc ctttgcccat gccggcgccg gcgcggaaac caccgcccag gcctgatccg
    14221 gccttagcgg tcaagggaaa ggggcgctgg ccactgcggc cggcgcccct ttcccttggc
    14281 ccggtgggca ggagatcaga acacccggcc ctcgtcgaaa tcagcccgct cgccgttcag
    14341 gtagaaatcg cccagcaccc gcccgcgcag cagcgccgga ttgtggttca gcagcgtgcg
    14401 cacattgcgc cagtgccggt cgaaggcgag ggtgatcgag gtggccgaac cgccgccggt
    14461 gtcgaacagc ctttgcgcgc cttcctgcgc cagccggccg accatgacct gcgccttgga
    14521 attggccagc tgcgcattca gcaccagcgc gtcgagcacg gccggatcgc tctcgccgcg
    14581 ggcgagggca gcggcggtga gatccagccg gcgcgccaca tcggcaatcg ccagatcggt
    14641 gatgtggacg agggcggaaa gatcgcccac tgtctgctgc acgaagggat cgccggcggc
    14701 ggtggccgaa ctgctgtgct tggccgagcg ggcgcgggtg gtggtgtagc tgatggcatc
    14761 ggccagcaca ttgcgcagga tgccgcccat caccgccacc agatgcagct gcgcgcgggt
    14821 ggcgccatgg cgctggcgga aggggttgtg gccattgcgc tcggccactt ccccgggccg
    14881 cacttccaca ttgtcgagca gcaccgtgcc gctggccgac agggtttggc ccatgccatc
    14941 ccaatcgtcg agcagggtga ggccggggcg gtctgccggg atcagcacat gggtgatctg
    15001 gccatcctcg cccaccgcgc tgaagctttc gaaatcggcg aagatggcgc cggtggcata
    15061 atatttggtg ccgttgaggc ggaaatgatc gccctcgcgg gtcagcttcg tgcgcagctg
    15121 gttggggcgc ggcgtgccga tctccatatg ggcgccgcca aacagccggc cggccagcac
    15181 ctgcggcgca tgggcccggc tgcgggcgga ggtggggtct gtcgccagcc cttcaaggaa
    15241 gccgaaatgg ctgcgcagcg catgggccac gttggaatcg gccgaggcca gcgccaccgt
    15301 cacttccacc tgatcggcca gcgtgccgcc ggggccgccc aggctttcgg gcacgcgcag
    15361 ccaggtgagg ccggtggtgc gcagccaggc gaacagctcg tgcggcaggt ggcgggcgcg
    15421 ctcgcgctcg gccgcgcctt cggccaccct tgccagcacc tcgggcaggc ccgcgagaat
    15481 ggcctcgcgg ctgagggcgg gcggggcggc cggccgctgc cggggggcgg gggcaagggt
    15541 gatcgtgtcg gacatcggtg ttctcctcag ggacgcggtg cggtcccggc attccgccaa
    15601 aagcctattg atatagtaga gtatatggct gtgcaaacgg gcattgcgcc ggaaagggcg
    15661 gatttcaggc aaaactgcgg gcgatgaacc agaaagtgcc cagccccacc agcccggccg
    15721 ccccggcatc tgccaccagc gcgcgcggca gcgcccgccc atggcgcacc agccaggccg
    15781 tgcctgtcac cagcgcgctg acgatggcca gctgggccag ttccaccccc aggttgaagc
    15841 ccagcaggat ctcggccagc cgggcggcgg gcaggcggtt ttccagcagg tcggcggcaa
    15901 agccaaagcc gtggatcagc ccgaacacca gcgtgacgac cagccgcacc cgcccggcat
    15961 cggccagctg cgccgacagc aggatatagc tgggcgcaaa cagccccatg cccgccagca
    16021 gcagcggcgg cagcgtgccg atgccggcca gccgaaggcc cgccatcgcc agcagcccgc
    16081 ctgctgcggc cagcgccacg gtggccggcc ggccggcggc ggcgctggcg ttttcggcgc
    16141 cgatcagggc aatggtcagc gccaccagcg cgtcgatata ttcggcatgg gggcggatca
    16201 gcccggtcac ggccagcgcc agggtgagcg aatggcccag cgtgaagccg gtgacgacga
    16261 acagcagatc gcgcagccgc cgcgacagca gcaccagccc gaccaggaag ctcatgtgat
    16321 cgacgccagt gaagatgtgc atcatcccca tgccgatgaa ggcgaacagg ccggcggtgg
    16381 ccagcgggcc ctggccggcc gcggcgatgg cggcggttgg ctggtcggtg ctcagcacgt
    16441 cctcggcaaa ggcgccgtcg gtgcggtgga tctgggcgaa atggaggtgg ccgggcacct
    16501 tgtcggccag caccgtgctg gtgatcgcca gcgccttgcc atcggcgcag gcaaaggcca
    16561 gctcggcgcg cagatagccg gcggccgcgc ccaccatgcg cggcggcgcg gcgctggcgc
    16621 agggctggcc atgatcggcc agcgtcagat gcgcggcgac ataggcgagg aaggcagcgc
    16681 ctgtgggcag gcggccatcg gccgtcagcc gccgggcctc gggggcggca agggtgaagc
    16741 tggcgcgggc gaagcggcca tcgatcagcc agctgctgtg cgattcgctg cgggtgtggg
    16801 cgctggccat gcccgccccc agcagcgcca gcagcgcgcc gatcagcgcc agcagcctca
    16861 ttcgtagccc ggccggatgg cgatatcggc cctggcgcgc aggaaacggt cggccgcgcc
    16921 gatcagcgcg cgggtttgcc gggcggtggc atcggccagc acccggtcgc gcaccagggc
    16981 aaaaggggcc gggcgcggcg gcgtgttggc atgcacggcc agaatgtgca gcccatcggg
    17041 cagcggcagc ggatcgctca ccgcgcctgc cggcaggcgc gcggcctcgg cgaacaggct
    17101 ggccccgaga tgggcgcggg cggcgaaata gaattcggcg ccatcggcca tccgcccggt
    17161 ggcggccagc cccagcgcgg ctgccacctg cggcgcgcca tgggcggcgc gcagggcctg
    17221 ggcggcggcg cgggcgcggg gggcctggct ggcgggcagg cggtattcat cgagggcgat
    17281 cacgccttca tcggcataat cggcggcatg ggcggcataa tcggcgcgca gggccgcctc
    17341 gtcgggcgcg gagagggtgg catcggccgc ttcctgcgct tccaccgcgc ccaccagcgc
    17401 ctggcgcact tccagcgtgt cggccggcag gcccagctcc accccgcgct gcacataaag
    17461 ctcctcgcgg atcatctcgt ccagcacctg ccggcgctgg gcgggcgtgg cggcggccag
    17521 gcccacgccg aagcgggcgc gcagctgatc gacataatcg gccatcagga tcggcacctg
    17581 gttgaccagc gccacatcct cgggcagcac gccggcgatg cgggtgccgt gcgcggtgaa
    17641 caggcccagg cccgccagca gcagccccgc caccgcgccc gccgcgcaca gccacagcgt
    17701 gcgcgcaggg ccagggcgca gccgctcagg cagcgggcgc atgggggctg gccagcgtgg
    17761 cgacataggc agccagcgcg cgggtttcgg cggcggacag gcggcgggca aaggccggca
    17821 tcaccccgcg ccggccatag gtgatggagc gggcgatggc gccggcgctg ccatcgccat
    17881 aaagccacac gcgatcggcc agatcgggcg cgccgatggc cggatcgcca tggccatcgg
    17941 cgccgtggca atcccagcag ccgcccttgc cggcatagat ctgcccgccg gcgcgggccg
    18001 ggccggtcag cggcgcccgg ccttccagat aatggagcca gtccaccagc agggcgatct
    18061 cgcccgggcc gagcggcacg aaaggctcgc tggcggaagg ccgggcgcgg ccgaaggcgg
    18121 gcatgtcggc cagggcctgg ccgcgggtgt cgccggcgcg gatgccgtgg cggatgatct
    18181 gctcgatctg gtccacccgg cccgatccat agagccagtc ggcatcggtg agatcgggcg
    18241 cgcccagatc cggccggccg cggccatcgg cattgtggca ggcggcgcag tggcgggcga
    18301 acagcgggcc cccttgggcg atggccgtgg cccgcagcgc cggcgttgcc agaatgcgat
    18361 cgggatcggc ggccaccaac tggcgcgcgc cctgccatgc ggccagcgca tgggccccgg
    18421 tgcccagcgc catcaggcc
//
LOCUS       DASRYE010000259         6814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_245371,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000259 DASRYE010000000
VERSION     DASRYE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245371"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2233)
                     /locus_tag="VFF98_13370"
     CDS             complement(2..2233)
                     /locus_tag="VFF98_13370"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZU64666.1"
                     /translation="MASPNISLPEEPGSRFVVLAPAPAAEETTPMASAGGLNFRYVAA
                     VVRANLRVIAFIFAVVMALAVVVTLVQTPRYTAKTTVQINNASSRVLKNQSDDKQDDE
                     EGATSTDTDRFLKTQVDIIKSRALALRVAQKLNLLHDTRLFKAEGASLPTPDLSPDAL
                     NDRVIGLLRGHTTVDLPRDSRVVSITVESTDPHLSADIANAYASEYIQSNLKRRFDSS
                     SYARDFIASQLAEMKGKLEESERGLNTYSRQAGLIHMAAASGGDNKSAAQGGSVTVSS
                     LMQVNQAANEAKARRIAAEARWDAIRAVPLLSASEVVSNMGINNLLGQKAQLEVALED
                     DRARHLDDYPTVRAKQAQLASINRQIMAVATSIRNAIQAEYKASVDAEAELNAQVNQL
                     KTATLSEQDRDVQYNLLARDVDSNRQIYDSLLQRLKELNASAGITISNISIVDPADVP
                     LLPSSPNLGKNLGVSLALALLLCTLTLVIKDQFDDSIRIPEDIETKLSLNLLGAIPQV
                     VDGNPEAGLDDPKSPLSEAYNSLRSVLLHATSHGLPRTLLITSAQPAEGKSTTCYAIA
                     LSLARMGKKVLLIDGDLRRPSMHRRIDCDNEQGLSTLLSSQTPLETIVRPTDVPGFFV
                     VPSGPVPPSPTEMLSSPRMRDVVQEACTLYDIVIIDSPPILGLADAPTMATIVEAVVF
                     VVEADRSRHGSLKAAMRRLRAHNSNVVGGVLTKFDPLRRGNQYSYYYGYNYYQYQYQY
                     KND"
     gene            complement(2252..2920)
                     /locus_tag="VFF98_13375"
     CDS             complement(2252..2920)
                     /locus_tag="VFF98_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZU64667.1"
                     /translation="MGLLGLSLLSGCYGTHNSALPHGPAAYQVAPSVEDGQARSAVQA
                     GDRLAVRVLGEPDLTSDQVWVDGNGRIQLPLAGEMQAGGLTPEVVRDEIARRLSARYV
                     RNPQVAVTIIEHAKQAITVEGEVQQAGLFEAPPGLTLLGALALAHSTTRDAALDEVVI
                     FRTVEGRRMAARFNIAEIRVGKAADPQVLAGDVVVVGRSAIKSVWHDFLQAAPAFSVY
                     YWLK"
     gene            complement(3277..3720)
                     /locus_tag="VFF98_13380"
     CDS             complement(3277..3720)
                     /locus_tag="VFF98_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64668.1"
                     /translation="MGYPLIAVLAASGLALGGGLASTGRSGDALPLTKAHLAPAAARE
                     VGCFVPVAKGRMPKLAQIRAARASGSCAKPLSAAMLADPAAGGAGAGAGGAGGAGAGA
                     GGAAGGAAAGGAGIGAGAIAGGVAAAGVAAGAAVAATSGGQTPQI"
     gene            complement(3835..6540)
                     /locus_tag="VFF98_13385"
     CDS             complement(3835..6540)
                     /locus_tag="VFF98_13385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZU64669.1"
                     /translation="MPHSRSHASGAGHRPRTADAAGPIGKAPLASAFGSRNATLLTGL
                     ALAAALFLGGNDATAPLAEAACEMLVGLLFTARFALAPGEDLRAIPGPAWAICGLLLA
                     LPALQLLPLPPGLWQALPGREIERDALALIGQAQSWRPLSLTPDRSFAALLGATNGAM
                     VLLMAAALGQRARNRLLLTILLVAGASVLVGSQQLTGAYNNFFRFYLPDQFFLEGFQI
                     NHNSEGDLLLIAILALAALIADSHALNTIRGERHRTMALMVAVTAVLAVAVVMTASRM
                     SMLLLAPTLAAALFIMRRWLRLEWRAWIRITGGLLALALAGGVVVAQSAVVQRALQRF
                     NLPADLRPEMWRMTAEAARDLFPLGGGMGSFPNVYQIHEKLEVVNEHYANRADNDYLE
                     LLLEAGVPGVLVLVAIVAILVRTVVQTRHGRRGKGLAQHQFALFSLALLAVHSVLDFP
                     LRPLSIAAVAACCVALLLPGRRPGPSITDPAASPATNEDQPRAAPAPIPLRESGLIAG
                     IGAAFVLCAAGSGLDRATLFSPALIDWVPAPFRSTALTMQAAHLYEAQDGRALLPAAG
                     AALARAPMEAANASLLGGAYGLLGRDAEAEQAYAVSARLGWRIPNTQAYWMFRQMELG
                     NGDAAADRWDALLRVNPALHTNLRLMLPVESSISLRPALLRKLAGNPPWLGRYIDEIE
                     LLPAEVMSNRAEILLELAENGHPLGCQRIASATYQLAEHDDLEGAKALWDAHCPQARG
                     GIVWNGDFSHSMQNTRGSAFDWIFLTSGDFNVMQLASADHRRQSLSVTSSSPFAQTFI
                     YQHIVVGPGTYEVAWRANDGTTLPDPAIVPLLGCAQQEPVRASAFRQTGSGRWAARLV
                     VPAPCDRPNLQFSILPSRAGVEFGSISMTQLSDQAGLPQQAEPAN"
BASE COUNT         1176 a         2212 c         2301 g         1125 t
ORIGIN      
        1 atcaatcatt tttgtattga tattggtatt ggtagtaatt gtagccgtag tagtatgagt
       61 attggttgcc tctgcggagg gggtcgaact tggtgaggac gccgccgacg acattggagt
      121 tgtgggcgcg caggcggcgc atggcggcct tgagggatcc atggcgggag cggtcggcct
      181 cgacgacgaa gacgacggcc tcgacgatgg tggccatggt gggggcatca gcgaggccga
      241 ggatgggcgg cgaatcgatg atgacgatat cgtagagggt gcaggcctcc tggacgacat
      301 cgcgcatgcg cgggctggag agcatttcgg tggggctggg cggcacgggc cctgagggga
      361 cgacgaagaa gcccgggaca tcggtggggc gcacgatggt ctcgagcggg gtttgcgagg
      421 agagcagggt ggagagaccc tgttcgttat cgcaatcgat gcgccggtgc atggagggcc
      481 ggcgcaagtc gccatcgatc agcaggacct ttttgcccat gcgggccagc gagagggcga
      541 tggcatagca ggtggtgctt ttgccttctg cgggctgggc gctggtgatc agcagggtgc
      601 ggggcaggcc gtggctggtg gcgtggagga gcacgctgcg cagcgagttg taggcttccg
      661 agaggggcga cttggggtcg tcgaggccgg cctcggggtt gccgtcgacg acctggggga
      721 tggcgccgag caggttgagg ctgagcttgg tctcgatatc ctcggggatg cggatggagt
      781 cgtcgaactg gtccttgatg accagggtga gcgtgcacag cagcagcgcc agggcgagcg
      841 agacgcccag gttcttgccg aggttgggcg agctgggcag cagcggcaca tcggccggat
      901 cgacgatgga gatattggag atggtgatgc cggccgaggc gttgagttcc ttcaggcgct
      961 ggagcaggct gtcgtagatc tggcggttgg aatcgacatc gcgggcgagc agattgtact
     1021 gcacgtcgcg gtcctgttcg ctcagcgtgg cggttttgag ctggttgacc tgggcgttga
     1081 gctcggcctc ggcatcgacg ctggccttgt attcggcctg gatggcattg cggatgctgg
     1141 tggccacggc catgatctgg cggttgatgc tggccagctg ggcctgtttg gcgcgcacgg
     1201 tgggataatc gtcgagatgg cgggcgcggt cgtcctcgag ggcgacttcg agctgggctt
     1261 tctggccgag caggttgttg atgcccatgt tgctgaccac ttcgctggcg ctgagcagcg
     1321 gcacggcgcg aatggcatcc cagcgggcct cggcggcgat gcggcgggcc ttggcctcgt
     1381 tggcggcctg gttgacctgc atcaggctgg agacggtgac cgagccgcct tgcgcggcgc
     1441 tcttgttgtc gccgccgctg gccgcggcca tgtggataag gccggcctgg cgcgaatagg
     1501 tgttgaggcc gcgttcggat tcttccagct tgcccttcat ctcggccagc tggctggcga
     1561 tgaagtcgcg ggcatagctg gagctgtcga agcggcgctt caggttggac tggatgtatt
     1621 cgctggcata ggcattggcg atatcggccg agaggtgcgg atcggtgctt tccacggtga
     1681 tcgacaccac gcgggaatcg cgcggcagat cgacggtggt gtggccgcgc agcaggccga
     1741 tcaccctgtc gttgagggca tcgggcgaga ggtcgggggt gggcaggctg gcgccttcgg
     1801 ccttgaacag gcgggtgtcg tgcagcaggt tcagcttctg cgccacgcgc agcgccagcg
     1861 cccggctctt gatgatgtcg acctgggtct tgaggaagcg gtcggtgtcg gtcgaggtcg
     1921 ccccttcctc atcgtcctgc ttgtcatcgc tctggttctt gagcacgcgg ctggaggcgt
     1981 tgttgatctg caccgtcgtc ttggcggtgt agcgcggcgt ctgcaccagc gtgaccacca
     2041 cggccagcgc cattaccacc gcgaagatga aggcgatgac ccgcaggttg gcgcgcacca
     2101 ccgcggcgac atagcggaag ttgagcccgc cggcactggc catgggcgtg gtttcttcgg
     2161 cagctggcgc cggcgccagc acgacaaagc gcgaacccgg ctcttccggc agggagatgt
     2221 tcggcgaggc catgtctgtt ccgtcagcct tttacttgag ccaatagtaa accgagaagg
     2281 caggcgcggc ctgcaggaag tcgtgccaca cgctcttgat tgccgagcgg cccaccacca
     2341 ccacatcgcc tgccagcacc tgcgggtcgg cagccttgcc cacccggatc tcggcgatgt
     2401 tgaaacgcgc ggccatccgc cgcccttcca ccgtgcggaa gatcaccact tcgtcgaggg
     2461 cggcatcgcg cgtggtgctg tgcgccagcg ccagcgcgcc cagcagcgtc agccccggcg
     2521 gcgcctcgaa caggcctgcc tgctgcacct cgccttccac cgtgatcgcc tgcttggcgt
     2581 gttcgatgat cgtcaccgcc acttgcgggt tgcgcacata gcgcgcgctc agccgccggg
     2641 cgatctcgtc gcgtaccact tcgggggtca gcccgccggc ctgcatctcg ccggccagcg
     2701 gcagctggat gcggccattg ccatccaccc atacctggtc cgaggtgaga tcgggctcgc
     2761 ccagcacgcg cacggccagg cggtcgccgg cctgcacggc gctgcgcgcc tgcccgtctt
     2821 caacgctggg cgccacctga taggccgccg gcccgtgcgg cagcgcgctg ttgtgcgtgc
     2881 catagcagcc cgacagcagg ctcagcccca gcagccccac agcagagcgc gcccagcgcg
     2941 cgtgccagcc acagtgccgg ccataagggc ctgtgcgctg atctgtcctg aaagaagtat
     3001 tccggatggc caaaagatct tcagcattca ttcggtctga tccgcccatg ccatcgccgc
     3061 agcgcatgcc cgcttcatcc tttacaggac agccccgtta gagcaacccg caaactatcc
     3121 tttatggtac atcaaccacc gattttgcag aaccaatccg gcacaagaac gccgctgcaa
     3181 aaacagcccc gcgcacaggc caaaagaaag acctccctca cgcagaagga ggcctttgcc
     3241 ttgtcgttcg ccttgccgtt ccggccaagc tgtccgtcag atctgcggcg tctgcccacc
     3301 agaagtggcc gccaccgcag cgccagcggc aacgcccgcc gccgcaacgc cgccggcaat
     3361 cgcgccagcg ccaatcccgg cgccaccggc cgcagcaccg cccgccgcgc caccagcgcc
     3421 tgcacccgcg ccgccggcac caccagcgcc cgcgccagca ccacccgccg ccggatcagc
     3481 cagcatcgcc gcagacagcg gcttggcgca ggaaccgctc gcccgcgccg cgcgaatctg
     3541 cgccagcttc ggcatccggc ccttcgccac aggcacaaag cagcccactt cacgcgccgc
     3601 cgcaggcgcc agatgcgcct tcgtcagcgg cagcgcatca ccagaacgac ccgtcgacgc
     3661 caggcccccg cccagcgcca aaccagacgc cgccaacacg gcaatcagag gatagcccat
     3721 gtcttctact cccagcgaca gtacataact gccaatccac acccacatat catacgtata
     3781 ttcactaggc cagtcgtttc gcagtcgcag caagcaactc gtctctcgtc aggctcagtt
     3841 tgccggttcc gcttgttgcg gcaaaccggc ctgatcggac aattgggtca tcgatattga
     3901 accaaattcg acacctgccc ggctgggcag gatcgagaac tgcagattcg gcctgtcgca
     3961 gggggcggga acgacaagcc gcgcggccca tctgccgctg ccggtttgcc ggaaggcact
     4021 ggcgcgcacc ggttcctgct gcgcacagcc cagcagcggc acaatcgccg gatcgggcag
     4081 ggtggtgccg tcattggccc tccaggccac ctcataggtt cccggcccga caacgatgtg
     4141 ctggtagatg aaggtctgcg cgaagggcga gctgctggtc accgacaggc tttggcggcg
     4201 atgatcggca gaggccagct gcatcacgtt gaaatcaccg gaagtcagga aaatccagtc
     4261 gaaggcggag ccgcgggtgt tctgcatgct gtgcgagaaa tcgccgttcc acacgatgcc
     4321 gccgcgcgcc tgcgggcaat gggcatccca cagggccttg gcgccttcca gatcatcatg
     4381 ttcggccagt tgataggtgg ccgaggcaat cctctggcag cccagcgggt gcccgttctc
     4441 ggccagttcc agcaggattt cggcccggtt gctcatgact tctgcgggca gcagctcgat
     4501 ctcgtcgata tagcggccca gccagggcgg gttgccggcc agcttgcgca gcagcgccgg
     4561 ccgcagcgaa atggagcttt ccaccggcag catcagccgc agattggtgt gcagcgccgg
     4621 gttcacgcgc agcagcgcat cccagcggtc ggccgcggca tcgccattgc ccagctccat
     4681 ctggcggaac atccaatagg cctgggtgtt ggggatgcgc cagcccagcc gcgccgacac
     4741 cgcataggcc tgctctgcct ccgcatcgcg ccccagcagg ccataggcac cgcccagcag
     4801 gctggcattg gccgcctcca tcggcgcccg ggccagcgcc gctccggcag ccggcagcag
     4861 cgcccggcca tcctgcgctt catacagatg tgcggcctgc atggtcagcg cggtggagcg
     4921 gaaaggggcc ggcacccagt cgatcagggc tggcgagaac agcgtggcgc gatcgagccc
     4981 gctgcccgcc gcgcacagca caaaggccgc gccgatgccg gcaatcagcc cgctttcgcg
     5041 cagcgggatc ggcgcgggcg cggcgcgggg ctggtcctcg ttggtggcag ggctggcggc
     5101 agggtctgtg atggaagggc cgggccgccg gccgggcagc agcagtgcca cgcagcaagc
     5161 cgccaccgcc gcgatcgaca gcggccgcaa cggaaaatcg agcaccgaat gcaccgccag
     5221 caaggccagc gagaacagcg cgaactgatg ctgcgccagc cccttgccgc gccgcccgtg
     5281 gcgcgtttgc accaccgtgc ggacaaggat cgcgacaatt gccaccagca ccagcacgcc
     5341 cggcacgccc gcttccagca gcagttccag atagtcgttg tcagcccggt tggcgtaatg
     5401 ctcgttcacc acctccagct tttcgtggat ctggtagacg ttgggaaagc tgcccatgcc
     5461 gccgcccagc gggaacagat cgcgcgcggc ctcggcggtc atccgccaca tttccgggcg
     5521 caggtctgcc ggcaggttga agcgctgcaa cgcgcgctgc accaccgcgc tttgcgccac
     5581 caccacgccg ccggccagcg ccagcgccag caaaccgccg gtgatcctga tccaggcccg
     5641 ccattccagc cgcagccagc gccgcatgat gaacaaggcc gcggccagcg tgggcgccag
     5701 cagcagcatc gacatgcgcg aggcggtcat caccacggcc accgccagca ctgccgtgac
     5761 cgccaccatc agcgccatgg tgcggtgcct ttcgccacgg atggtgttga gcgcgtgcga
     5821 atcggcaatc agcgcggcca gtgccaatat ggcgatcaac agcagatcgc cctcgctgtt
     5881 atggttgatc tggaagcctt cgaggaagaa ctgatcgggc agatagaagc ggaagaaatt
     5941 gttgtaggcg ccggttaatt gctgcgagcc gaccagcacc gaggcaccgg ccaccagcag
     6001 gatcgtcagc agcaggcggt ttctggcccg ctggcccagg gcggcggcca tcagcagcac
     6061 catcgccccg ttggtcgccc ccagcaaggc ggcaaagctg cgatcggggg tgagcgagag
     6121 tggccgccag gattgcgcct ggccgatcag cgccagcgcg tcgcgctcga tctcgcgccc
     6181 gggcagcgcc tgccacaggc ccggcggcag cggcagcaat tgcagcgccg gcaaggccag
     6241 cagcaggccg cagatcgccc aggccgggcc gggaatcgcg cgcagatcct cgccgggcgc
     6301 cagggcgaat ctggccgtga acaacaggcc gacgagcatt tcgcaagccg cttcggccag
     6361 cggcgctgtg gcatcattgc cgcccaggaa cagcgccgcc gccagcgcca gcccggtcag
     6421 caaggtggca ttgcggctgc caaaggcgct tgcaagcggc gccttgccga ttgggccggc
     6481 cgcatcggct gtgcgcggcc ggtggccagc cccggaggca tgagagcggg aatgcggcat
     6541 ctttcgaaca tcctcgtggc cgggcgcgcc atgttagaaa gccaaaacgg cgcaaggcga
     6601 tatgaaatat taccaggccg gcaaatttgt gccaacgtta tcctcggcta acacgtaggt
     6661 aaaaaccttg tccacaggct ccgcgcagga atgcgatgag ttgcttgctg cgactgcgaa
     6721 acgactggcc tagtgaatat acgtatgata tgtgggtgtg gattggcagt tatgtactgt
     6781 cgctgggagt agaagacatg ggctatcctc tgat
//
LOCUS       DASRYE010000260        14736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_87222,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000260 DASRYE010000000
VERSION     DASRYE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87222"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFF98_13390"
     CDS             complement(<1..364)
                     /locus_tag="VFF98_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64670.1"
                     /translation="MTASPSIVARPARLGNLEFVVLMAMIQALQALAVDSMLPALGQM
                     SVDLHVANPNQRQLVVGVFLICAGLGSLIPGTLADRFGRRRALFFCLSSYVTLTTACA
                     LATDFPLLLGARALLGLAC"
     gene            479..1621
                     /gene="dinB"
                     /locus_tag="VFF98_13395"
     CDS             479..1621
                     /gene="dinB"
                     /locus_tag="VFF98_13395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZU64671.1"
                     /translation="MSQARMPPAPAGAPAGEMPVGLRKIIHIDMDAFYASVEQRDEPA
                     LKGLPLAVGHAGARGVVAAASYEARRFGVRSAMASAKALRLCPQLVFRPPRFEAYRAV
                     SRQIRAIFAEYTPLIEPLALDEAYLDVTEDLKGLGNATRIAELIRARIRAETGLTASA
                     GVSYNKFLAKIASDQNKPDGLCVIRPGEGAAFVAALPVRRFHGVGPRGAEKLAKLGIH
                     TGADLRALGLEVLRAHFGAQADYLYRAARAIDLRAVSPDRPRKSLGGERTFSRDLSSG
                     ASLRAALEETIALVWGRIEKAGVSGRTVTLKLRHADFSIHTRAASSPTPITTEAGFAV
                     AARALLEQALPLPQPVRLMGLTLSALGEPAAGEAEAPPADSQLSLF"
     gene            complement(1607..2101)
                     /locus_tag="VFF98_13400"
     CDS             complement(1607..2101)
                     /locus_tag="VFF98_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZU64672.1"
                     /translation="MLQLIATLLPAPVHRAGLRLAHRARVLWWRWRRPRLYGCRVIAL
                     NAAGEVLLVRHSYGPQGWMPPGGGVKRGEDPVLAACRELEEEIGCALAGARVIEETLD
                     DLHGAGNHVFMVGGRCTGQPRPDGREIVAAAFFPPAALPADLAGALAGRLPGWAALAA
                     SEEA"
     gene            complement(2089..2706)
                     /locus_tag="VFF98_13405"
     CDS             complement(2089..2706)
                     /locus_tag="VFF98_13405"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZU64673.1"
                     /translation="MADIIHTPAASNRDIARWLAAHITTALATAPEVAITVPGGSTPF
                     PVFEELARLDLPFARIAIWPGDDRIVPEDHPASNVGRIRALLQPVGARIVPLAEGAAP
                     PRFAVALLGMGGDGHIASLFPNTNPRADAPAQVLRLTPDPLPPEAPFDRLTLTIPALV
                     NADAIAFLIRGEEKRAIFAAAAEGRNDLPVARLLAATPAQVTCFS"
     gene            complement(2857..3312)
                     /locus_tag="VFF98_13410"
     CDS             complement(2857..3312)
                     /locus_tag="VFF98_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZU64674.1"
                     /translation="MTNHNAHAALVDSLNGLLADLFTLYLKTKNFHWHVRGANFHDLH
                     LLFDAQATEIFALTDIIAERVRKNGAATLTSIGSIAARSQIQDQPAADLAPEAMIAEL
                     RDDNAKLVTILRAVKADATAAGDNATEGAADDWTDQAQQRVWFLTQLLA"
     gene            complement(3408..3980)
                     /locus_tag="VFF98_13415"
     CDS             complement(3408..3980)
                     /locus_tag="VFF98_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64675.1"
                     /translation="MNGLLFAFVLALLAGIGARDQMTVAALSAGLGRHAGLLLVALAT
                     AALACAAAAWAAGEMAPVLGEGLRLPVASFAMVAAGLESLLLGPRRAPKEPTRSLSAA
                     AIVLLADQILDAARFLVLAMALVTHSPAACAVGGALASAAVLGLAWLEGGRVLGARRL
                     LRRLRLAAGAVLLLAGGLLGAWVAGWIGGP"
     gene            complement(3977..4384)
                     /locus_tag="VFF98_13420"
     CDS             complement(3977..4384)
                     /locus_tag="VFF98_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HZU64676.1"
                     /translation="MDHAAPSAQPGPSPVASRHPDIAALVAHFYARARADALLGPVFA
                     HGVADWPQHLRAITAFWAAQLRGRGTYQGQPMAAHLRHAGLIRPEMFARWLALWEEET
                     AARMAPEDAARLQEKARRIAEVLQRALAGDGAR"
     gene            4662..5135
                     /locus_tag="VFF98_13425"
     CDS             4662..5135
                     /locus_tag="VFF98_13425"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZU64677.1"
                     /translation="MPIQPPAVLFVCLGNICRSPLAEAALRHAATGAGLALAVDSAGT
                     GNWHVGGPPDPRAQAEARRHGIDISTYRARQVEAADFHRFSHILALDHQNLADLARLR
                     PADAPAALSLALDLLPGHEGQAVADPYFGEAADFARCWAEVSALAEALVRSWQRA"
     gene            complement(5143..5703)
                     /locus_tag="VFF98_13430"
     CDS             complement(5143..5703)
                     /locus_tag="VFF98_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64678.1"
                     /translation="MAINRSLFQQAYFVSSIDAAARKWADAFGAGPFFMVRHHACAEF
                     TYRGTAQEADVSYAFGYLGETMIQFIEQHDDTPSIYRDLFGPGEEGFHHVAYLVSDFA
                     AERQRWLDRGYTLASELYADEVNAAYFDTRDINGGFTEIHGDPAHILGLFAVWRRAHE
                     ARDADTPATVEMGDMAAYARAALMAK"
     gene            5845..6738
                     /locus_tag="VFF98_13435"
     CDS             5845..6738
                     /locus_tag="VFF98_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZU64679.1"
                     /translation="MADAAPALEVPPFSVVATGYQFVEGPRVAADGTLWFSDLLAGGY
                     HRIAPDGTASAFATGRQWIGGAVLVEDGTLVVSGRGGLARLDPATGRLTPLPLATGDP
                     FELCANDLEAAPDGGIIGGTIDFASIMETHQPPRPGTLFHRAPDGRLTILRQDVFASN
                     GLGFSPCGRWLYHSETSRGVWRYPLGADGLPDCRPGQAHLLAAMEDSDGLVVDAEGAI
                     WVAGWASASLRRLLPDGREDRCLSLPAFPHLVSLAFGGPDLNALYVSTGGNEAAPGLG
                     GVVKIRVEVPGLRDRRAQVPA"
     gene            6735..7553
                     /locus_tag="VFF98_13440"
     CDS             6735..7553
                     /locus_tag="VFF98_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU64680.1"
                     /translation="MSAPETSAPLIRLERLEGGIALVTLARAEARNALSLAMVRQLRA
                     VLAEVAADAALRVVVLAGEGKAFCAGLDLKQVILGEEAPTGPIANMALQELYAGLMLD
                     IHRLRQPVIAAVQGAAVGAGMGVALAADIRIAAPCAQFLVGAVRIGLSAGECGISYHL
                     PRLVGAGRAFEIMLTGRPVTGDEAAAIGLASRLVAEDALRQEALATAQAIAANAPYSI
                     KHTKQVMWANLEAPSLAAAIELENHVQVAALLSADFAEACRAFAEKRPPVFRGA"
     gene            7686..9059
                     /locus_tag="VFF98_13445"
     CDS             7686..9059
                     /locus_tag="VFF98_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU64681.1"
                     /translation="MTDVQAQQRARYPGNTWADIARRDGELPPVLARQSNPPQGTADI
                     PFTRYTSPDFFDLEIERMWKRVWQFATRAEYLAEEGDYYVYDLARLSVVLVRTAEGLK
                     GYINSCLHRGTKLKPSGGAGWGGSLRCPYHAWEWHLDGTLKNIPCAHEFPHLDKDRAQ
                     LPQVRVEEWNGNVFLNFSPEGPSLAEYLEGLPQHFAHWDLSGWYVHAHVRKAVPGNWK
                     LAQEAFMEAYHTPFVHPEMTHVVGDHNMQHDILSDHISRDLCAMATPSPTSPVAMSEQ
                     QLLEAMLVGDGTMIGSRMVVPEGKTARWVMAEQLKAQMQRDFGLDYTGHSVPEMIDSL
                     KYAVFPNANIYPAPGLCLIQTFRPAGHDHDRALFDQLVLRPKPADGDYTPASVVEIGE
                     DEPFTSVEGIDPFLASVLDQDTDIMRWQREGMYASAKGAETLSNYQESRIRHFHETLD
                     KYLSGDR"
     gene            9136..9963
                     /locus_tag="VFF98_13450"
     CDS             9136..9963
                     /locus_tag="VFF98_13450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64682.1"
                     /translation="MDLELKGKKVIISAATRGLGRRIAERFLAEGAIVSFCGRRAHAG
                     ETNPSDSEVFSNPLAGDGVAEAEAALQRLGTAYGSVVDCGYFEQVTGWVEQAAARMGG
                     IDIVVSNASALGGIPRSRKGWDINYHVDLLSAVALFDTALPHLKASGQGAFVQMSTIT
                     AIENHAFGESGLSYGAIKAALINYTHQLSQDYMKFGIRCNCVCPGPIYVEDGSWGFLE
                     EAMPDYYEANRAKHPAGRFGRPDEIADAVLYLASARASWITGVNLTVDGGFTRNVKY"
     gene            9968..10981
                     /locus_tag="VFF98_13455"
     CDS             9968..10981
                     /locus_tag="VFF98_13455"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZU64683.1"
                     /translation="MAPTLPPPRFDTPLTRLVGIRLPIVQGGMQWVGRAELASAVANA
                     GGLGMLTALTQPSPTALAEEIERCRGLTDQPFGVNLTVLPTRSPPPYAAFLQAAIDAR
                     VPVLETAGMVPAEFLAAAKAAGMCILHKCTTLRHALSAQKKGVDIVSIDSFEAAGHPG
                     EEDIGAMVLVPAAARALAIPVIASGGIANGAGLAAALALGAAGVNMGTRFCATAEAPI
                     HPAIKQALVAAGAADTNLIFRTLNNTGRVLKNAVSDEVVAIEQRPGGARFEDIAHLVA
                     GARGRAALEAGEVDAGLVWASQALGLIDDIPTCADLLARMARECADHLSRAAAACGGL
                     VPV"
     gene            11050..11763
                     /locus_tag="VFF98_13460"
     CDS             11050..11763
                     /locus_tag="VFF98_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64684.1"
                     /translation="MSPPAAPPAPLGALPFERDPAVWIAHSTSHVMPRQQRSRQALER
                     IVVAALKLFAERGFEATPVSAIAAEAGVPVGTIYQRFADKSALLRTVIEGFRALRMGE
                     IAALCQSPAAQAASPAEVVRLHVDIIFSAFLTDDGLLRLIERRRLDDEATHREQSEAN
                     RQVAEWIAALLIAKLPGRNPAELRQQVLYTHNIIRGAVVWAILPAAGEWEQGLKVHDP
                     AFATAALDMALAYLGIKRG"
     gene            11833..12048
                     /locus_tag="VFF98_13465"
     CDS             11833..12048
                     /locus_tag="VFF98_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64685.1"
                     /translation="MSEDKPLIAARAAAAQYLRAQGYDADAAYVEAGQSDDTREVQIA
                     LAMHRILHVVPSPPTRYKGRRLVGEEC"
     gene            12127..13203
                     /locus_tag="VFF98_13470"
     CDS             12127..13203
                     /locus_tag="VFF98_13470"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZU64686.1"
                     /translation="MRSFWAPMVHQLSPYTPGEQPAVPGLIKLNTNELPFGPSPAVIA
                     AIQAAALDGLQLYPDPQSIALCDGLARYHGLARDCVFVGNGSDEVLAHAFAALLQHDR
                     PLLFPDVTYSFYPTYCRLFGITHETVPLNGAMQIDVADYERPAGAIILPNPNAPTGSA
                     IGLAALERLLAAHPDAPVVIDEAYVDFGAHTAIPLIARHPNLLIVRTMSKSRGLAGLR
                     VGYALGQPHLIEALVRVKDSFNSYPLDRLAQAGALASLGDEPYFQRSLQTIVANRAQL
                     VQGLEALGFLLLPSSANFVFARHPGHSGAQLALALRAARILVRHFNAPRIDSFLRITV
                     GDAGQVQALLDALAVILRDQAGNT"
     gene            complement(13218..13997)
                     /locus_tag="VFF98_13475"
     CDS             complement(13218..13997)
                     /locus_tag="VFF98_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU64687.1"
                     /translation="MDNLRSHDDCDIAPMPIAALASEHADGAHIAAHRHLRAQLIHTL
                     SGVMTVGTRDGSWVVPPGRALWMPALRDHAIRIAGDVAMRTVFVRGDARPALPQACEV
                     IEVSPFLREAIVAATRIPLDYAQGGRDERVMQLILDEIVAAPRLDLHVHLPRDARLMR
                     ICQRIIADPAAGARLEQLAAEIHVSGRTLARLFHRELGMSFGAWLRRTRLLLSLPRLA
                     AGMSVLEVALEHGYDSPSAYTAMFRRTLGVSPTAYLQHRAD"
     gene            14084..>14736
                     /locus_tag="VFF98_13480"
     CDS             14084..>14736
                     /locus_tag="VFF98_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU64688.1"
                     /translation="MPYRFPPAIAAAALATTLLGGCLSAPRTPPASTLRVPDQLVDQP
                     APGAAPLPAAWWRLYRDPALDALVGEALANNRDLRAAAAHWQEARALLGKARAERLPT
                     TRLSAGAGEGSTLEDQIAAAARGNDRVRTGPRYDLGSAVSWEPDLFGRLQAHVRAARA
                     EAQASGALLDGVRIAVAADVVGAWLRACGLAHQAAVAQDLLAQAQRGRDLAESLRAAG
                     "
BASE COUNT         2304 a         5205 c         5004 g         2223 t
ORIGIN      
        1 aacaggccag ccccagcagc gcccgcgcgc ccagcagcag cggaaaatcg gtggccagtg
       61 cgcaggccgt ggtcagcgtg acataggagg acaggcagaa gaacagcgcc cgccgccggc
      121 cgaagcgatc ggccagcgtg ccgggaatga ggctgcccag ccccgcgcag atgagaaaca
      181 cccccaccac cagctggcgc tgattgggat tggccacatg cagatccacc gacatctgcc
      241 ccagcgccgg cagcatggaa tccaccgcca gcgcctgcaa tgcctggatc atcgccatca
      301 gcacaacgaa ttcgagattg ccaaggcggg cgggcctggc aacgatagag ggggaagctg
      361 tcatcgcggg cgcttattgc gccttcccgg cccgcttggc tagccctgtg gcgccaagaa
      421 taggtatctg aaaagagccg ggctggcccc cgcgcggccg gcgcgctaga agccggccat
      481 gagccaggca cgcatgcccc ctgcccctgc cggcgcgccc gccggcgaga tgcctgtggg
      541 cctgcgcaag atcatccata tcgatatgga cgccttctat gccagcgtgg aacagcgcga
      601 cgagcccgcg ctgaagggcc tgccgctggc cgtgggccat gccggcgcgc gcggcgtggt
      661 ggccgccgcc agctatgagg cgcgccgctt tggcgtgcgc tcggcgatgg cttcggccaa
      721 ggcgctgcgg ctgtgcccgc agctggtgtt ccgcccgccg cgcttcgagg cctatcgcgc
      781 cgtctcgcgc cagatccgcg cgatctttgc cgaatacacc ccgctgatcg agccgctggc
      841 gctcgacgag gcctatctcg atgtgaccga ggatctgaag gggctgggca atgccacccg
      901 catcgccgag ctgatccgcg cgcgtatccg cgccgaaacg ggccttaccg ccagcgccgg
      961 ggtcagctac aacaagttcc tcgccaagat cgcttccgac cagaacaagc ccgatgggct
     1021 gtgcgtgatc cgcccgggcg aaggggccgc tttcgtggcg gcgctgccgg tgcggcggtt
     1081 tcatggggtg gggccaaggg gggcggaaaa gctggcgaag ctgggcatcc acaccggcgc
     1141 tgatctgcgc gcgctggggc tggaggtgct gcgcgcccat ttcggcgccc aggccgatta
     1201 tctctaccgc gccgcccggg cgatcgacct gcgcgccgtc agccccgacc gcccccgcaa
     1261 gagcctgggc ggcgagcgca ccttcagccg cgatctttcc tcgggcgcat cgctgcgcgc
     1321 ggcgctggaa gagacgatcg cgctggtgtg ggggcggatc gagaaagcgg gcgtttccgg
     1381 ccgcacggtg acgctgaagc tgcgccatgc ggatttttcc atccacaccc gcgccgcctc
     1441 cagccccacg ccgatcacca ccgaggcagg ttttgccgtc gccgccaggg cgctgctgga
     1501 acaggccctg ccgctgcccc agcccgtgcg gttgatgggg ctgacgctat cggcgctggg
     1561 cgagccggcc gcgggcgagg ccgaagcgcc gcccgcagac agccagctaa gcctcttctg
     1621 acgccgccag cgccgcccag cccggcagcc gccccgccag cgcgccggcc agatccgccg
     1681 gcaacgcggc gggcggaaag aaggcggcgg ccacaatctc gcgcccatcg ggccggggct
     1741 ggccggtgca gcgcccgccc accatgaaca catggttgcc tgcgccatgc agatcatcga
     1801 gcgtttcctc gatcacccgc gcgccggcca gcgcgcagcc gatctcttcc tccagctcgc
     1861 ggcaggcggc cagcaccgga tcttccccgc gcttcacccc gccgcccggc ggcatccagc
     1921 cctgcggccc ataggaatgg cgcaccagca gcacctcgcc cgccgcattc agcgcgatca
     1981 cccggcagcc atacagccgc ggccgccgcc agcgccacca cagcacccgc gcccgatgcg
     2041 ccagccgcag cccggcgcga tgcaccggcg ccggcagcaa ggtggcgatc agctgaagca
     2101 tgtgacctgc gccggggtgg cggccagcag gcgcgccacg ggcagatcat tgcgcccctc
     2161 ggcggcagcg gcaaagatcg cccgcttctc ctcgccgcgg atcaggaagg cgatggcatc
     2221 ggcattgacc agcgccggga tggtcagcgt cagccggtcg aacggcgcct cgggcggcag
     2281 cggatcgggg gtcaggcgca gcacctgcgc gggggcatcg gcgcgcgggt tggtgttggg
     2341 gaacagcgag gcgatatggc catcgccccc catgcccagc agcgccacgg caaagcgggg
     2401 cggcgcagcg ccttccgcca gcggcacgat gcgcgcgccc accggctgca gcagcgcgcg
     2461 gatgcggccg acattgctgg cgggatggtc ttcgggcacg atgcgatcat cgcccggcca
     2521 gatggcgatg cgggcaaagg gcagatccag ccgcgccagt tcctcgaaca ccggaaacgg
     2581 ggtcgatccg cccggcacgg tgattgccac ctcgggcgct gtggccaggg cggtggtgat
     2641 gtgcgcggcc agccagcggg cgatgtcgcg gttggaagcg gcgggggtgt ggatgatgtc
     2701 tgccatggga tttctcccta gcatcgctgc cgggccgggg gaaccgcagg gcaaacctat
     2761 gcgggccccg gccggcccga cgggaagggc aggccgggcc ggggcccctg aaccggccgc
     2821 cgggagggcg gatggcggcc ggtcatccgg tggttgtcag gccagcagct gggtgaggaa
     2881 ccacacgcgc tgctgggcct ggtcggtcca gtcgtcggcg gcgccttcgg tggcattgtc
     2941 gccggcggcg gtggcatcgg ccttcaccgc gcgcaggatc gtcaccagct tggcgttatc
     3001 atcgcgcagt tcggcgatca tcgcctcggg ggcaaggtcg gcggcgggct ggtcctggat
     3061 ctggctgcgc gcggcaatgc tgccgatcga ggtgagcgtg gccgcgccat tcttgcgcac
     3121 gcgctcggcg atgatatcgg tcagggcgaa gatctcggtc gcctgcgcgt cgaacagcag
     3181 gtgcaggtcg tggaagttcg ccccgcgcac atgccagtgg aagttcttgg tcttgaggta
     3241 gagcgtgaac agatcggcca gcaggccgtt caggctgtcc accagggcgg catgggcgtt
     3301 atggttggtc atcgctgcgt ctccgtctgt gtccgtgagg ggggtatagg ggcacgagtg
     3361 gtaagggtca tctgcaacaa tatggtgatt gtgatcggct ttttcgatca gggcccgcca
     3421 atccagccgg ccacccaggc gcccagcagc ccgcctgcca gcagcagcac cgcgcccgcc
     3481 gccagccgca gacggcgcag cagccggcgc gcgcccagca cgcgcccgcc ctccagccag
     3541 gccagcccca gcaccgccgc gctggccagc gcgccgccca cggcgcaagc cgcggggctg
     3601 tgcgtcacca gcgccatcgc cagcacgagg aagcgggcgg catcgaggat ctgatcggcc
     3661 agcagcacga tggcggccgc gctcagcgag cgggtgggct cctttggcgc ccggcgcggg
     3721 cccagcagca gcgattccag ccctgcggcc accatggcaa agctcgccac cggcaggcgc
     3781 agcccctcgc ccagcaccgg cgccatctcg cccgccgccc aggccgccgc cgcgcaggcc
     3841 agcgccgccg ttgccagcgc caccagcagc agcccggcat gccggcccag ccccgccgac
     3901 agcgcggcca ccgtcatctg atcgcgcgcg ccaatgcccg ccagcagcgc cagcacgaaa
     3961 gcgaacagca ggccgttcac cgcgccccat ccccggccag cgcgcgctgc agcacctcgg
     4021 cgatgcggcg ggccttttcc tgcaggcggg cggcatcctc gggcgccatg cgcgcggcgg
     4081 tctcctcctc ccacagggcc agccagcggg cgaacatctc gggccggatc agcccggcat
     4141 ggcgcaggtg ggccgccatc ggctggccct gataggtgcc ccgcccgcgc aattgcgccg
     4201 cccagaaagc ggtgatcgcg cgcaggtgct gcggccagtc tgccacgcca tgggcaaaca
     4261 ccgggcccag cagcgcatcg gcgcgcgcgc gggcatagaa atgggccacc aaagcggcga
     4321 tatccgggtg gcggctggcc accgggctgg ggccgggctg ggcagaaggg gcggcatgat
     4381 ccatccctgc ctgagtagcg ccttgcccgg cgtgggcaag cccccctcgg ttcaccgggc
     4441 aggcgcgtcg ctttgtcgcc ggcctattgt ctatcgcccg aattgagtta tcacccatgc
     4501 tgcggcttca gtgcaggagg agccgtggtt gcgtaaaatg ggcgaagcct cgcggcggcg
     4561 catgaacgcg aggcttcgct cgcccccaat gatgatcccc ccttcacggt gacaggccct
     4621 tcacggtgac aggatggccg caaggcgcta gatctctttc catgcctatc cagccgcctg
     4681 ccgtgctctt cgtctgcctt ggcaacatct gccgctcgcc gctggccgaa gcggcgctgc
     4741 gccatgccgc cacgggcgcc ggcctggccc ttgcggtcga ttcggccggc accggcaact
     4801 ggcatgtggg cggcccgcca gacccgcgcg cccaggccga ggcccgccgc cacgggatcg
     4861 acatttccac ctatcgcgcc cggcaggtgg aagcggcgga ttttcaccgc ttcagccata
     4921 tcctcgcgct cgatcaccag aacctggctg atctggcccg cctgcgcccg gccgatgccc
     4981 cggccgccct gtcgctggcg ctggatctgc tgcccggcca tgaagggcag gccgtggccg
     5041 acccctattt cggcgaggca gccgatttcg cccggtgctg ggcggaagta tctgccctgg
     5101 cagaagcgct ggtgcgcagc tggcagcggg cctgagccgc tcctatttcg ccatcagcgc
     5161 cgcccgcgca taggcggcca tgtcgcccat ctccaccgtc gccggcgtat cggcatcgcg
     5221 tgcctcatgc gcgcgcctcc acaccgcgaa caggcccagg atatgcgccg gatcgccgtg
     5281 gatctcggtg aagccgccgt tgatgtcgcg cgtgtcgaaa taggccgcgt tcacctcatc
     5341 ggcatagagc tcgctggcca gcgtatagcc cctgtcgagc cagcgctggc gttcggcggc
     5401 aaaatcggac acgagataag cgacatggtg gaacccctcc tcgcccgggc cgaacaggtc
     5461 gcgatagatc gagggggtgt cgtcgtgctg ctcgatgaac tggatcatcg tctcgcccag
     5521 atagccaaag gcataggaga catccgcctc ctgcgcggtg ccgcgatagg taaattccgc
     5581 gcaggcatgg tggcgcacca tgaagaaggg cccggcgcca aaggcatcgg cccatttgcg
     5641 cgccgccgca tcgatgctgg agacgaaata agcctgctgg aacaggctgc gattgatggc
     5701 catggccggc cctctcctct tgtggtttgc cgcaaggtgc cgcgcaatgg ccccgccggc
     5761 agcctgtgga atgtgagagg tggaggcggc ccggcgcccg cgccataacg tgaacataaa
     5821 atcacattca tcgggagact gcctgtggcc gatgccgccc ccgcgcttga ggtgccgccc
     5881 ttttcggtag tggccacagg ctatcagttt gtggaagggc cgcgcgtggc ggccgatggc
     5941 acgctgtggt tcagcgatct gctggccggc ggctatcacc gcatcgcccc cgatggcacc
     6001 gccagcgcct ttgccaccgg ccgccagtgg atcggcgggg cggtgctggt ggaagatggc
     6061 acgctggtcg tctcgggccg gggggggctg gcgcggctgg atccggccac gggcaggctg
     6121 acgccgctgc cgctggccac gggcgatccg ttcgaactgt gcgccaatga tctggaagcc
     6181 gcgcccgatg gtggcatcat cggcggcacg atcgattttg ccagcatcat ggaaacgcat
     6241 cagcccccac gccccggcac gctgtttcac cgcgcgcccg atggccggct gacgatcctg
     6301 cgccaggatg tgtttgcttc caacgggctg ggcttcagcc cctgcgggcg ctggctctat
     6361 cattcggaaa ccagccgggg ggtgtggcgc tatccgctgg gggccgatgg cctgcccgat
     6421 tgccggccag gccaggccca tctgctggcc gccatggagg atagcgacgg gctggtggtg
     6481 gatgccgaag gggcgatctg ggtggccggc tgggccagcg ccagcctgcg ccgcctgctg
     6541 cccgatggcc gcgaggacag gtgcctcagc ctgcccgcct tcccccatct cgtcagcctg
     6601 gcgttcggcg ggccggatct caacgcgctt tatgtttcca ccggcggcaa tgaagccgcg
     6661 ccggggctcg gcggcgtggt gaagatccgc gtggaagtgc cgggcctgcg cgatcgccgc
     6721 gcacaggtgc cggcatgagc gcgccggaaa ccagcgcgcc cctcatccgg ctggagcggc
     6781 tggagggcgg catcgccctt gtcaccctgg cccgcgccga ggcgcgcaat gcgctgagcc
     6841 tggcgatggt gcggcaattg cgcgcggtgc tggccgaagt ggccgccgat gcggccctgc
     6901 gcgtggtggt gctggccggc gagggcaagg ccttttgcgc cgggctcgat ctcaagcaag
     6961 tgattctggg cgaggaagcg cccacagggc ccatcgccaa catggcgctg caagaactct
     7021 atgccgggct gatgctggat atccaccgcc tgcgccagcc ggtgatcgcc gccgtgcagg
     7081 gcgcggcggt gggcgccggc atgggcgtgg cgctggcggc cgatatccgc attgccgcgc
     7141 cttgcgcgca atttctggtg ggcgcggtgc gcattggcct gtctgccggg gaatgcggca
     7201 tttcctatca cctgccgcgg ctggtggggg cgggccgcgc cttcgagatc atgctgaccg
     7261 gccggccggt gacgggcgac gaggccgccg ccattggcct tgccagccgg ctggtggcag
     7321 aagacgcgct gcgccaggaa gcgctggcca ccgcgcaggc gatcgccgcc aacgcgccct
     7381 attccatcaa gcacaccaag caggtgatgt gggccaacct ggaagcgccc agccttgccg
     7441 ccgccatcga gttggaaaac cacgtgcagg tggccgccct gctgtctgcc gattttgccg
     7501 aggcctgccg ggcctttgcc gaaaagcgcc cgccggtgtt ccggggagcc tgacggcagg
     7561 cgcgcccccc aacctcaccc tttgcgcagg ttgggcgaag ggggggttgc aaaacatgat
     7621 tattggttca tgtttttccc atccgacggg ggacccatcc cccgcgcgtc tttcgggaga
     7681 aagcgatgac cgacgttcag gcgcagcagc gtgcgcgcta tccgggcaac acctgggccg
     7741 atatcgcccg ccgcgatggc gaactgcccc ccgttctggc ccggcagagc aacccgccgc
     7801 aaggcaccgc cgatatcccc ttcactcgct acaccagccc ggatttcttc gatctggaga
     7861 tcgagcggat gtggaagcgg gtgtggcaat ttgccacccg cgccgaatat ctggccgagg
     7921 agggcgacta ttacgtctat gatctggcgc ggctttcggt ggtgctggtg cgcacggccg
     7981 aagggctgaa aggctacatc aattcctgcc tccaccgcgg caccaagctc aagccatcgg
     8041 gcggggcggg ctggggcgga tccttgcgct gcccctatca tgcctgggaa tggcaccttg
     8101 atggcacgct gaagaacatc ccctgcgccc atgaattccc ccatctcgac aaggaccggg
     8161 cccagctgcc gcaagtgcgg gtggaagaat ggaacggcaa tgtgttcctc aacttcagcc
     8221 cggaaggccc ctcgctggcg gaatatctgg aagggctgcc gcagcatttt gcccattggg
     8281 atctgtccgg ctggtatgtc catgcccatg tgcgcaaggc cgtgcccggc aactggaagc
     8341 tggcgcagga ggccttcatg gaggcctatc acaccccctt cgtccacccc gagatgaccc
     8401 atgtggtggg cgaccacaac atgcagcacg atatcctctc cgaccatatc agccgcgacc
     8461 tgtgcgccat ggccaccccc agccccacct cgccggtggc aatgagcgag cagcagctgc
     8521 tggaagccat gctggtgggc gatggcacga tgatcggcag ccgcatggtg gtgcccgagg
     8581 gcaagaccgc ccgctgggtg atggccgagc agctgaaggc gcagatgcag cgcgatttcg
     8641 gcctcgacta caccggccat tccgtgcccg aaatgatcga cagcctgaaa tatgccgtct
     8701 ttcccaatgc caatatctat cccgccccgg gcctgtgcct gatccagacc ttccgccccg
     8761 ccggccacga ccatgaccgc gccctgttcg accagctggt gctgcgcccc aagcccgccg
     8821 atggcgatta caccccggcc agcgtggtgg aaatcggcga ggacgagccc ttcaccagcg
     8881 tcgagggcat cgatcccttc cttgccagcg tgctcgatca ggataccgat atcatgcgct
     8941 ggcagcgcga gggcatgtat gccagcgcca agggcgccga gaccctgtcg aattatcagg
     9001 aatcacgtat ccgtcatttc cacgagactc tcgacaagta cctttcgggc gacagataaa
     9061 ccggcaccgg ccacccaacc acaacaaagg ggggcaaacc cccgccatag aagaacaacc
     9121 gggagaaaca gcgccatgga tctggaactg aagggcaaga aggtgatcat cagcgccgcc
     9181 acgcgcggcc ttggccggcg catcgcggaa cgcttcctcg ccgagggggc gattgtcagc
     9241 ttctgcggcc ggcgcgccca tgccggcgag accaacccgt ccgacagcga ggtgttctcc
     9301 aatccgctgg ccggcgacgg ggtggccgag gccgaagcgg ccctgcagcg cctgggcacc
     9361 gcctatggca gcgtggtcga ttgcggctat ttcgagcagg tgaccggctg ggtggaacag
     9421 gccgccgcgc gcatgggcgg gatcgatatc gtcgtctcca atgccagcgc gctgggcggc
     9481 ataccgcgca gccgcaaggg ctgggatatc aactatcacg tcgatctcct gtcggccgtg
     9541 gcgctgttcg acaccgcgct gccgcatctg aaggcctcgg gccagggcgc cttcgtgcag
     9601 atgtccacca tcaccgccat cgagaaccat gcctttggcg aaagcggcct gtcctatggc
     9661 gcgatcaagg ccgcgctgat caactacacc catcaattgt cgcaggatta catgaagttc
     9721 ggcattcgct gcaattgcgt gtgcccgggg cccatctatg tggaagatgg cagctggggc
     9781 ttcctcgaag aggccatgcc cgattattac gaagccaacc gcgccaaaca tcccgccggg
     9841 cgcttcggcc ggcccgacga gatcgccgat gccgtcctct atctcgcctc ggcgcgggcc
     9901 agctggatca ccggggtcaa cctcaccgtc gatggcggct tcacccgcaa tgtgaaatac
     9961 tgagccgatg gcccccaccc tgccgccgcc gcgcttcgac acgcccctca cccgcctggt
    10021 cggcatccgc ctccccatcg tgcagggggg catgcaatgg gtgggccggg cagagctggc
    10081 cagcgccgtt gccaatgccg gcgggctggg catgctcacc gccctcaccc agccctcgcc
    10141 caccgcgctg gccgaggaga tcgaacgctg ccgggggctg accgaccagc ccttcggcgt
    10201 caaccttacc gtgctgccca cccgcagccc gccgccctat gccgcctttc tgcaggccgc
    10261 catcgatgcc cgcgtgccgg tgctggaaac ggccggcatg gtgccagcgg aattcctcgc
    10321 cgcggccaag gcggccggca tgtgcatcct ccacaaatgc accaccttgc gccatgcgct
    10381 gtcggcccag aagaagggcg tcgatatcgt ctcgatcgac agtttcgagg cggccggcca
    10441 cccgggcgag gaagatatcg gcgccatggt gctggtgccg gcagccgcgc gcgcgctggc
    10501 gatcccggtg atcgcctcgg gcggcatcgc caatggcgcg gggctggcgg cagcgctggc
    10561 gctgggggcc gccggcgtca acatgggcac gcgcttttgc gccacggcag aagcgcccat
    10621 tcacccggcg atcaaacagg cgctggtggc cgccggcgct gccgacacca acctcatctt
    10681 ccgcacgctg aacaacaccg gccgcgtgct gaaaaacgcc gtgtcggacg aggtggtggc
    10741 gatcgagcag cgcccgggcg gggcccgctt cgaggatatc gcccatctgg tggcaggcgc
    10801 gcgcggccgc gcggcgctgg aagcgggcga ggtggatgcg gggctggtct gggccagcca
    10861 ggcgctgggg ctgatcgacg acatccccac ctgcgccgac ctcctcgccc gcatggcgcg
    10921 cgaatgcgcc gaccacctca gccgcgcggc cgcggcctgc ggcgggcttg tgcctgtgtg
    10981 aggcacgctg tgatcggcgc cgcgatcggc actggcgccg gggcgccggc agcgttaggg
    11041 caaggggcga tgagcccgcc cgctgccccg cctgcccccc tgggcgccct gcccttcgag
    11101 cgcgacccgg cagtgtggat cgcccattcc accagccatg tgatgccccg ccagcagcgc
    11161 agccgccagg cgctggaacg catcgtcgtg gccgcgctca agctgtttgc cgagcgcggc
    11221 ttcgaggcca ccccggtcag cgccattgcc gccgaggcag gcgtgccggt gggcaccatc
    11281 taccagcgct ttgccgataa atccgccctg ctgcgcacgg tgatcgaggg ctttcgcgcc
    11341 ctgcgcatgg gcgagatcgc cgctttgtgc caatcgcccg cagcgcaagc cgccagccct
    11401 gccgaagtgg tgcgcctgca tgtggacatc atcttcagcg ccttcctcac cgacgacggg
    11461 ctgctgcgcc tgatcgaacg ccgccggctg gacgacgagg ccacccaccg cgaacagagc
    11521 gaggccaacc ggcaggtggc cgaatggatc gccgccctgc tgatcgccaa gctgcccggg
    11581 cgcaaccctg cggaactgcg ccagcaggtg ctctacaccc acaacatcat ccgcggcgcg
    11641 gtggtgtggg ccattctgcc ggccgccggc gaatgggaac agggcctcaa agtgcacgac
    11701 cccgcctttg ccaccgccgc gctggacatg gcgctggcct atctgggcat caaacggggg
    11761 tgaaggcctg ccgctgctga gaccgctgcc gcggctggtc atcccccatc cgatcgttta
    11821 gaagccgcca ggatgagcga agacaagccc ctgatcgccg cccgcgccgc tgctgcccaa
    11881 tatctgcgcg cgcaaggcta tgacgcggat gcggcctatg tcgaggcggg ccagtcggac
    11941 gacacgcgcg aggtgcagat cgcgctggcc atgcacagga tcctccatgt cgtgcccagc
    12001 ccgccgaccc ggtacaaggg ccgccgcctg gtcggcgagg aatgctgaca tccgggcaag
    12061 gcccatacat tgcttgagac gccggatagc ggccctgcct gccggggccc gaaaaggggc
    12121 aaactgatgc gttcattctg ggccccgatg gtccaccagc tttcgcccta cacgccggga
    12181 gagcagcccg ccgtgcccgg cctcatcaag ctgaacacca atgaattgcc ctttggcccc
    12241 tctccggcgg tgatcgcggc aatccaggcg gccgcgctgg atgggttgca gctgtatccc
    12301 gatccgcagt cgatcgcgct gtgcgatggg ctggcgcgct atcacgggct tgcgcgcgat
    12361 tgcgtgtttg tcggcaatgg gtcggacgag gtgctggccc atgcctttgc cgccctgttg
    12421 cagcacgatc ggccgctgct gtttcccgat gtgacctaca gcttctatcc cacctattgc
    12481 cgcctgtttg gcatcaccca tgaaaccgtg ccgctgaacg gggccatgca gatcgatgtg
    12541 gcggattacg agcgcccggc cggcgccatc atcctgccca accccaacgc gcccaccgga
    12601 tcggccatcg gcctggcagc gctggagcgc ctgctggccg cgcatcccga tgccccggta
    12661 gtgatcgacg aggcctatgt cgatttcggc gcgcacaccg ccatccccct catcgcgcgc
    12721 catcccaacc tgctgatcgt gcgcacgatg tcgaaatcgc gcgggctggc cgggttgcgg
    12781 gtgggctatg cgctgggcca gccgcatctg atcgaggcgc tggtgcgcgt gaaggacagt
    12841 ttcaactcct atccgcttga ccggctggcc caggccggcg cgctggcctc gctgggcgac
    12901 gagccctatt tccagcgcag cctgcaaacc atcgtggcaa accgcgcgca gctggtgcag
    12961 gggctggaag cgcttggctt cctcctcctg ccatccagcg ccaacttcgt gttcgcccgg
    13021 cacccgggcc acagcggggc gcaactggcg ctggcgctgc gcgcggcgcg gatcctggtg
    13081 cgccatttca acgcgccgcg gatcgacagc ttcctgcgca tcacggtggg cgatgccggg
    13141 caggtgcagg cgctgctgga tgcgctggcg gtgatcctgc gcgatcaggc cggcaacacc
    13201 tgaaccaggc ggcccgctca atccgcgcgg tgctgcagat aggccgtggg tgaaacgccc
    13261 aatgtgcggc ggaacatcgc cgtataggcg ctggggctgt cgtagccatg ctccagcgcc
    13321 acttccagca ccgacatccc cgccgccagc cgcggcaggc tgagcagcag ccgcgtgcgg
    13381 cgcagccatg cgccaaagct catccccagc tcgcggtgga acaggcgggc cagcgtgcgc
    13441 ccgctcacat ggatctccgc cgccagctgt tccagccttg cgccggcggc aggatcggcg
    13501 atgatcctct ggcagatccg catcagccgc gcatcgcggg gcaggtgaac atgcaggtcg
    13561 aggcggggcg cggcaacgat ctcgtcgagg atcagctgca tcacccgttc atcgcgcccc
    13621 ccttgcgcat aatcgagcgg gatgcgcgtg gcggccacga tcgcctcgcg caggaagggc
    13681 gacacttcga tcacttcgca ggcttgcggc agggccggcc gcgcatcgcc ccgcacgaaa
    13741 accgtgcgca tcgccacatc gccggcaatg cggatggcat gatcgcgcag cgccggcatc
    13801 cacagggccc ggcccggcgg caccacccag ctgccatcgc gggtgccaac ggtcatcacc
    13861 ccgctcagcg tgtggatcag ctgggcgcgc aagtgccgat gggcggcgat atgcgcgcca
    13921 tcggcatgct cgctggccag cgcggcgatg ggcatggggg cgatgtcgca atcatcatgg
    13981 ctgcgcagat tgtccatatc ccgacgacag atgtcactcc ggcgcgagaa gggcaagcgc
    14041 cttgccgcta cacacgcagc ccgcaaccat cagagggcct gccgtgcctt accgttttcc
    14101 ccccgccatc gccgccgcgg cgctggccac cacgctgctg ggcggatgcc tgtctgcgcc
    14161 gcgaacgccg cctgcctcca cccttcgcgt gccggatcag ctggtcgatc aacctgcgcc
    14221 cggcgccgcg ccgctgcctg cggcctggtg gcgcctttat cgcgatccgg cgctcgatgc
    14281 gctggtcggc gaggcgctgg ccaacaatcg cgatctgcgc gcggcggccg cccattggca
    14341 ggaagcgcgc gccctgctgg gcaaggcgcg ggccgagcgc ctgcccacca cccgcctgtc
    14401 tgccggcgcc ggcgaaggga gcaccctgga ggaccagatc gccgccgccg cccggggcaa
    14461 cgacagggtg cgcaccggcc cccgctacga tctgggcagc gcggtttcct gggagccgga
    14521 cctgttcggc cggctgcagg cccatgtgcg ggcggcgcgg gccgaagcgc aggcctctgg
    14581 cgcgctgctg gatggcgtgc gcatcgcggt ggcggccgat gtggtgggcg cctggctgcg
    14641 cgcctgtggc ctcgcccatc aggcagccgt ggcgcaagac ctgctggcac aggcgcagcg
    14701 cggccgcgat ctggccgaaa gcctgcgtgc ggcagg
//
LOCUS       DASRYE010000261        17598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_245465,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000261 DASRYE010000000
VERSION     DASRYE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 17598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17598
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245465"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFF98_13485"
     CDS             <1..1155
                     /locus_tag="VFF98_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZU64689.1"
                     /translation="VAGTLAFAPFDYEAAPPAATMAAYRACRAPFAETGSPALPQGLN
                     FGAHLFWAEERYPEAMRAATLVPWAQYWAWVFSGVAVSEATSLGCHSDLWAPATGSWS
                     SLAKARGWATRFAPLARAGDVIGTLRPEIAAATGLDAGIKVLAGIHDSNAALLAARGH
                     ADIAGKEATVLSTGTWFIAMRLPASPVATATLPEARDCLVNVDAFGQPVPSARFMGGR
                     EIETLIEIDTRRVDIKPDQPALLAAVPAVLAAGAMHLPTLAPGCGPFPQGRGRWINPP
                     ADWYERRAAACLYAALVADRALDLIGAEERLLVEGRFAEAEVFVRALASLRPDTQVFV
                     ANAHNDVSFGALRLIDPALAPAGGLRQVAPLDLALDTYRNRWLAEVDSTP"
     gene            1152..2294
                     /gene="lldD"
                     /locus_tag="VFF98_13490"
     CDS             1152..2294
                     /gene="lldD"
                     /locus_tag="VFF98_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HZU64690.1"
                     /translation="MIIASVADMRAAARRRLPRFLFEYMDGGSYAETTLRRNVADLEA
                     IALRQRVLRDVSGLDLSTELFGQRQSLPVALAPIGLAGLNARRGECQAVRAAEQAGVP
                     FTLSTVSACPLAEVAQAASRPFWFQLYMIRDRAFMNDLLDQASAAGCSALVFTVDMPV
                     PGSRYRDYHTGLAGTGGLKGAAWRAMQAMARPGWAWDVGLMGRPHHLGNVAPVLKGKT
                     GIEDFFAWMRNNFDPSINWRDLDFIRARWQGPLIIKGILDPEDAREAARLGADGIVVS
                     NHGGRQLDGVLSSARALPPIADAVGDQLTVLADGGIRSGLDVVRMLALGARGVLLGRA
                     WSYALGAGGGTAVTQMLDIIRAEMRVAMALTGCTRIADIDRTILAT"
     gene            2319..3383
                     /gene="rhaT"
                     /locus_tag="VFF98_13495"
     CDS             2319..3383
                     /gene="rhaT"
                     /locus_tag="VFF98_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HZU64691.1"
                     /translation="MGGNPALGVIFHWIGGFSSASFYVPYKRIRGWNWEIFWLAGGLF
                     SWALAPWLFAALRTHDLLGVLAAAPANALFWCWFWGAMWGFGGLTFGLTMRYLGLSLG
                     MAVALGLTTVIGTMGPPIFHGTIGQIAGTTSGRLTLAGIGVTLLGILVVAGAGRAKER
                     EMGGTVSATLAEFNLTKGLAIACFSGVMSGCFAWGLDAGQPIRELTLAAGTDPLSQGL
                     PVLCVVLAGGLTTNALWCGYLIWRNRSAGQFLAALPQGGAVPSLAANWLLAALGGTLW
                     YGQFFFYTMGESQMGRYGFSSWTLHMASIILFSTLWGFALKEWRGASAPTRRRVWAGI
                     GLLVGATVVIGAGNMLAAGQ"
     gene            3448..4239
                     /locus_tag="VFF98_13500"
     CDS             3448..4239
                     /locus_tag="VFF98_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HZU64692.1"
                     /translation="MHATQRESLILDALRPTGFVSYRDLEAQLAASPATIRRDLARLE
                     AEGRITRVHGGAKLAGDPAEPPAPAAPRQQLLGTPFDLAITQNLAAKQAIGRAAAALC
                     EPGEGIMVDGGTTTLQMCPHLAGLDLQVLTNSLHIAGALLPQPGTRILLPSGAVFREQ
                     NIILAPAGEDSMPRFHAPKLFMGAAAVGPRGVMQHDVILVAAERRLMDRAEQVILLVD
                     ASKFGSSSGGIVCALDEVDILITDAAITPEQAAMVEAAGVRLIIA"
     gene            4399..5049
                     /gene="mtgA"
                     /locus_tag="VFF98_13505"
     CDS             4399..5049
                     /gene="mtgA"
                     /locus_tag="VFF98_13505"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZU64693.1"
                     /translation="MALGGFIGLSVLATLAYRVVPPPVTITMLADPNGIAKQWTPLSE
                     IDRSMVDAVIAGEDARFCEHHGFDQAGIQYALRRDMNGGKLRGGSTISQQTAKNTFLW
                     QGSGWDRYLRKALEAWFTVLVEHLWGKRRIMEVYLNVAETGIGTYGVEAGAQRYYRHS
                     AAHLSQVEAARIAAALPSPKKRAVVGASGFTRRHGNAIAAREAVVRTGALDSCVYR"
     gene            complement(5139..8741)
                     /locus_tag="VFF98_13510"
     CDS             complement(5139..8741)
                     /locus_tag="VFF98_13510"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64694.1"
                     /translation="MRYSTLAALAASTIAIAAVVAQPAYAQDTTSAIAGQVTAESGAP
                     VGGAKVTILHTPTGTRETVTTDGTGNFAMRGLRVGGPYTVTVEAAGQPTQIYDNVMLT
                     LGDTFSLPVIMQKQIIVAGLQARGGRQLITGSETSFRAKDIQDIVSARRDVRDVMRRD
                     LLSGFNPNAGGVSIAGGNIRTQRFSVDGVQMQDSFGLNYGGLPSSRGIVSLEMIDQLS
                     VKAAPFDISEGNFQGGAVNVVLKSGGNKFHMTAFGNFGGPGLNGTKTADTLVVPGSGA
                     SVLPTKILPFTNFGGSISGPIIKDKLFFAAAYERLTEGTPDLWGPMDGTASNAVTNLH
                     TGDFTVPSGTYLNGSNYYPYGQNVIAWNAANPTNQFVDPGTGTFGNVMPGLTTLYNTF
                     NKYYGNYNIGTVPTSINEVDEKYAAKLDWNVTEGHRVSVSYIHHENVLPSFYSGVGGS
                     NSATSPYILLLSDFYKLTEHTDAFSGQWNGKWTDRLSSEVRVAYKYYRRGQDAYNGPD
                     YAQMNVCMDPTSSSLAAAASGTQYANETTCSNGSPTVRMGPDTARQANKFRNRIYTYQ
                     GNLSYRAGAHTFKLEYDHNYSTLYNLFVYGGSGLAGSGGPNGLYYFDSMADFAAKQAN
                     ELALTTTTTGNKNDGFVSWAYHTNTVGFQDTWKAQSNLTINGGFRYDWYTADNTIAQN
                     ANFTARYSVLYPGLTNTATLDGRHKFEPRIGFNWQPTHTLKVAGGLGLFAGGLSDVFI
                     SNNYSNSGAPQGINGSGAAITSVDIVRLANGSCIDRSTGTVNPTWNGASICADALSNV
                     NGATIPSDVLNYAAHNTSVLANTYTNFLDPKFKLPAQWKYNITLSWHPDFTKLGLGDG
                     WTFRTDVLFSDTQSGVRWVDLRAQPLVVNGVVQTTPDGRTRYGGTLNTANGAVQPGSN
                     YDMELTNTKRGISRVFAIGATKSWKDFDVSASYTHTRVHDIAGALSSSTVSSSYSVPT
                     DDANSGGAYGRSAFEISSIVRANINFHHKFFGDNETRLGINWELRSGQPYSITMNDYT
                     TNSTNGRSGVFGTVLNTTSQLLYVPNFANCSGLTCGNVTFDSASTMANLQKLVNGTKL
                     YNYQGAIAPKNLMTGPSYNKIDLNFAQQIPVPRLGRGKLTALFSIENFLNMLNRDWGT
                     YQDIGATAVVKVACVAVGGNACGQYLYSSYSSPKAQAYIKPSLYAIRAGVRFDF"
     gene            9029..10018
                     /locus_tag="VFF98_13515"
     CDS             9029..10018
                     /locus_tag="VFF98_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZU64695.1"
                     /translation="MQAHSHSTAHDHGHGHTHSHDHAHGHGHGHHHGHHHHHGDTHGT
                     AFAVAVTLNLAFLVVETGAGIWGHSMALLADAGHNFSDVLSLLLSWGASALARRPPSA
                     RYTYGLKSSSILAALANAALLWVALGAILIETLRRLFEPASAEGTVMIWVAAAGILVN
                     GLSAVLFARGSKEDLNLRAAFQHLLADAAVSAGVVVAGIAVALSGWSWIDPATSLAIT
                     ALIGWGSWGLLRESVQLGLLAVPAGINESAVRAELAAKPGVTAVHDLHIWPMSTTETA
                     LTAHLVMPAGHPGDEWLHGVAHALEHEHGIGHVTLQVETEEGHECALHSDGVV"
     gene            complement(10015..11346)
                     /locus_tag="VFF98_13520"
     CDS             complement(10015..11346)
                     /locus_tag="VFF98_13520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZU64696.1"
                     /translation="MTQAIELVLTGGTVHTPNGPENCDVAVSGGKIVGLGHFPDAARR
                     IDCTGLDILPGVIDSQVHFREPGLVHKEDLESGSRAAVLGGITAVFEMPNTNPNTDTA
                     EAVHDKLARAHHRMWCDHAFYVGATTANAAHLAELERIPGTAGVKIFMGASTGNLLVA
                     EDAELARVLASGVRRVAIHAEDEARMNARKDFRVEGDPSSHPVWRDDESALLATRRIL
                     KLARAARRPIHILHVTTPAELELISQHRDIASCEVTPQHLTLRGEEAYPRLGTLAQMN
                     PPIRSGAHVDGLWHWLRQGVPDVIGSDHAPHTLEEKAKPYPASPSGMPGVQTLLPLML
                     DHVLNGRMTLARLIDMTSAGVQRLFGLQGKGRIAAGYDADFTVVDLKGRFTVSEEWLA
                     SRCGWSPYTGMELKGRVMGTIVRGHQVVWEGALAAAAIGEPLRFAAALPEG"
     gene            11459..12220
                     /locus_tag="VFF98_13525"
     CDS             11459..12220
                     /locus_tag="VFF98_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZU64697.1"
                     /translation="MTTLAARALIRLAPQAPEENPATFLQGLVTNDVVKGPLPAYAAL
                     LSPQGKVLFDFLVWADGADLLIDCAADSAEALAKRLSLYRLRRAIAITRETALAVHWS
                     ASEVPGAVPDPRLAALGWRWLAPADAAPATAAPADAAWLAHRLAQGVAEGAELADTLW
                     LECNAVELNGVSFTKGCYVGQENTARMNWRQKVNRRLVVVPLERSTPARQRAAYPALG
                     LAVDHLPLAAITPDLAPTWLALAEAEETDPAAPSH"
     gene            complement(12144..12767)
                     /locus_tag="VFF98_13530"
     CDS             complement(12144..12767)
                     /locus_tag="VFF98_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZU64698.1"
                     /translation="MTLRRRLSSDESRAAALAAARLILIEEGAQAVTLKSVAARMGRT
                     HANLLHHFGSAAELQKALAAYMADNICAAIGSAVIASRLGVGTARAVVDLAFDAFEHE
                     GGGQLASWLRITGNEEALAPIVDAVHQMVEELHPFGSEATRHVTLTLVLLALGDALMG
                     APLAAALAMPRATARDMAERLLISETARLGLSPPPRPVRATSVPDPA"
     gene            12864..13676
                     /locus_tag="VFF98_13535"
     CDS             12864..13676
                     /locus_tag="VFF98_13535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZU64699.1"
                     /translation="MLKHNEINIRNIRFASAPAARWWLAGDPVATAWHNALSASFPRG
                     EAYFIEAVRANRAGTTGALARQIDAFIAQEANHSREHHRFNRHLAEAGYDMAGIDAAL
                     AAFMAENRSQWDVVNLAATIALEHFTAIIAHDLLARPDYLAGAEPEMARLWRWHAVEE
                     IEHKAVAFDTWLHATRRWSGWQRWRLRCLVMLKVSRNFLTRRSADALCLLAQDGITGW
                     RARARLAAYLLGKPGVIRRILPVWCGFFRPGFHPWQTDDRALIAAVDLQAGV"
     gene            complement(13686..14336)
                     /locus_tag="VFF98_13540"
     CDS             complement(13686..14336)
                     /locus_tag="VFF98_13540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZU64700.1"
                     /translation="MFTRTERLFLRPIWAEDWKDLSAMLADPGATGRLAAGLWGAEPE
                     AASVFAWQEARAPQLLITRPHGGGVETVGAIGLFRRPGERAGARRGEAEFQIFVAPAW
                     RGQGLGAEAAGALLPLARLLGYRRLQAGHAVEDVASGGLLRKLGFRPTGEMALVAAGH
                     PAEMGPGLRSTLVRCALDLPALTGLAGDEDREGDDWGGDDWGGDDDLGGAPTRRAA"
     gene            complement(14611..14880)
                     /gene="rpmA"
                     /locus_tag="VFF98_13545"
     CDS             complement(14611..14880)
                     /gene="rpmA"
                     /locus_tag="VFF98_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZU64701.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKKFGGQEVIGGNIIIRQRGTR
                     VYPGVNVGMGKDHTLFSLAEGRVRFHDGKLGRKYVSVDVVAEAAE"
     gene            complement(14902..15204)
                     /gene="rplU"
                     /locus_tag="VFF98_13550"
     CDS             complement(14902..15204)
                     /gene="rplU"
                     /locus_tag="VFF98_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZU64702.1"
                     /translation="MFAVVRTGGKQYRVAAGDKIAVEKLAGEAGETITLADILLAGED
                     GQIADASKVTVSAEIIAQAKSEKVIVFKKRRRHNYRRRNGHRQQLTLLRILSVAAA"
     gene            15502..16095
                     /locus_tag="VFF98_13555"
     CDS             15502..16095
                     /locus_tag="VFF98_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64703.1"
                     /translation="MHTRCHIAAPLAKAPQAGGPAPLRIGAALALAGLLAGCSTGGYP
                     SLRPRAEEQASYRTTPPEAPRPPEALSADLGQQLARLVEEARQGHAAFLARLPHAEAA
                     LAANGDSPAGTSSWSEANEALAELDTARAPAGQALAAITRLYVDDRLDHALDDAAAGN
                     GLARPAGARLDAAQAQVAALVAEEDGKLAALQARLPG"
     gene            complement(16118..16582)
                     /locus_tag="VFF98_13560"
     CDS             complement(16118..16582)
                     /locus_tag="VFF98_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZU64704.1"
                     /translation="MSGRADVRLALAAFDPATALGGFAAAHEAAGGIASFIGQVRAGE
                     GVEALELQHYAPLTLPGMEALAADVLARWDLAGLLIVHRSGVMRPGEPIVLVAAAARH
                     RRAAFEAADFAMDHLKSDSWFWKRERRAGQWHWIEPRAEDAADLARWGQARG"
     gene            complement(16579..16821)
                     /locus_tag="VFF98_13565"
     CDS             complement(16579..16821)
                     /locus_tag="VFF98_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZU64705.1"
                     /translation="MGGAARLVFLGRLADLAGVGEQAVLPGPLADVLAGLAPALAEAL
                     LAPRIRLALNGALVAPVGLVLAEGDELAFLPPVSGG"
     gene            complement(16825..>17598)
                     /locus_tag="VFF98_13570"
     CDS             complement(16825..>17598)
                     /locus_tag="VFF98_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZU64706.1"
                     /translation="RDMIELVRALGRQLGQGLDELTLTTNGTHLAAHAEALYAAGVRR
                     VNVSLDTRDREVFARIARRDQLPAVLEGIAAARAAGLAVKLNTVALKGVNEAEIPDLV
                     RWAHGAGHEITLIEVMPLGEVEEDRFDHYLPLSAVRAQLAQAFTLEADGHRSGGPARY
                     VRLAETGGRLGFITPLTDNFCEGCNRVRVTATGQLFACLGGREQVDLRAALRSAGPEA
                     ALDEALDEAMRMKPARHHFAIDRAGAAPALARHMSMTGG"
BASE COUNT         2716 a         6105 c         5865 g         2912 t
ORIGIN      
        1 gtggctggta cattggcctt cgcccccttc gattacgaag ccgcgccgcc cgctgccacc
       61 atggccgcct atcgcgcctg ccgcgcgccc tttgccgaaa ccggatcgcc cgccctgccg
      121 caggggctca atttcggcgc ccatctgttc tgggccgaag agcgctatcc cgaggcgatg
      181 cgcgccgcca cgctggtgcc ctgggcgcaa tattgggcct gggtgttctc gggcgtggcg
      241 gtcagcgagg cgaccagcct tggctgccat tccgatctct gggcgccggc caccggcagc
      301 tggtcgtcgc tggccaaggc gcgcggctgg gccacccgct ttgccccgct ggcccgcgcc
      361 ggcgacgtga ttggcacgct gcggccagaa atcgccgcgg ccacggggct ggatgccggg
      421 atcaaggtgc tggccggcat ccatgattcg aacgccgccc tgctggccgc gcgcggccat
      481 gccgatatcg ccggcaagga agccacggtg ctgtccaccg gcacctggtt catcgccatg
      541 cgcctgccgg ccagccccgt ggccaccgcc accctgcccg aagcgcgcga ttgcctcgtc
      601 aatgtcgatg cctttggcca gccggtgccc tcggcccgct tcatgggcgg gcgcgagatc
      661 gagacgctga tcgagatcga cacccgccgg gtggatatca agcccgatca gcccgccctg
      721 ctggccgccg tgcccgccgt gctggccgca ggggccatgc atctgcccac gctggcgccg
      781 ggctgcgggc cgtttccgca aggaaggggc cgctggatca acccgccggc cgactggtat
      841 gaacggcgcg ccgcggcctg cctctatgcc gcgctggtgg ccgatcgcgc gctcgatctc
      901 atcggcgcgg aagagcgcct gctggtggaa ggccgctttg ccgaggccga agtgttcgtg
      961 cgggcgctgg cctccttgcg gcccgacacg caggtctttg tggccaatgc ccataatgac
     1021 gtgtccttcg gcgccttgcg gctgatcgat ccggcgctgg cgccggccgg cgggctgagg
     1081 caggttgccc cgctcgacct tgccctcgac acctatcgca accgctggct tgccgaagtg
     1141 gatagcaccc catgatcatt gcctctgtcg ctgatatgcg cgccgccgcg cgccgccgcc
     1201 tgccccgctt cctgttcgaa tatatggatg gcggcagcta tgccgagacc accttgcgcc
     1261 gcaatgtggc agacctggaa gcgattgccc tgcgccagcg ggtgctgcgc gatgtttcgg
     1321 ggctggatct gtctaccgag ctgttcggcc agcgccagag cctgccagtg gccctggcgc
     1381 ccattggcct tgccgggctg aacgcacggc gcggcgaatg ccaggcggtg cgcgcggcag
     1441 agcaggccgg cgtgcccttc acgctgtcca ccgtctcggc ctgcccgctg gccgaagtgg
     1501 cgcaggcggc cagccgcccg ttctggttcc agctctacat gatccgcgac cgcgccttca
     1561 tgaacgacct gctcgatcag gcgagcgctg ccggctgctc ggcgctggtg ttcacggtgg
     1621 acatgcccgt gcccggcagc cgctaccgcg attatcacac cggccttgcc ggcaccggcg
     1681 ggctgaaggg cgcggcctgg cgcgccatgc aggccatggc ccggcccggc tgggcgtggg
     1741 atgtggggct gatgggccgc ccgcaccacc tgggcaatgt ggcccctgtg ctgaaaggca
     1801 aaaccggcat cgaggatttc ttcgcctgga tgcgcaacaa tttcgacccc tcgatcaact
     1861 ggcgcgatct cgatttcatc cgcgcgcgct ggcaggggcc gctgatcatc aagggcatcc
     1921 tcgaccccga agacgccagg gaagccgcaa ggctgggcgc cgatggcatc gtcgtctcca
     1981 accatggcgg gcgccagctg gatggcgtgc tgtcgtcggc ccgcgcgctg ccgcccattg
     2041 ccgatgccgt gggcgaccag ctgaccgtgc tggccgatgg cggcatccgt tccggcctcg
     2101 atgtggtgcg catgctggcg ctgggggcca ggggcgtgct gctggggcgg gcgtggtcct
     2161 atgcgctggg ggcgggcggc gggaccgccg ttacccagat gctcgacatc atccgggccg
     2221 agatgcgcgt tgccatggcg ctgaccggct gcacgcggat cgccgatatc gatcgcacga
     2281 tcctcgccac ataagcataa acaagggaga gcaaggccat gggtggcaac ccggcactgg
     2341 gtgttatctt tcactggatc ggggggtttt cctcggccag cttctatgtg ccctacaagc
     2401 ggatccgcgg gtggaactgg gagatcttct ggctggcggg cgggctgttc agctgggcgc
     2461 tggcgccctg gctgtttgcc gcgctgcgca cgcatgatct gctgggcgtg ctggcggcgg
     2521 cgcccgccaa cgcgctgttc tggtgctggt tctggggggc gatgtggggc tttggcggcc
     2581 tcactttcgg gctcaccatg cgctatctcg gcctgtcgct gggcatggcg gtggcgctgg
     2641 ggctgacaac cgtgatcggc acgatgggcc cgcccatctt ccacggcacc atcggccaga
     2701 tcgccggcac cacctctggc cggctgacgc tggcgggcat tggcgtcacg ctgctgggca
     2761 ttctggtggt ggccggcgcc ggccgcgcca aggaacgcga gatgggcggc accgtcagcg
     2821 ctactctcgc cgaattcaac ctgacgaagg ggctggccat cgcctgcttc tcgggcgtca
     2881 tgtcgggctg ctttgcctgg gggctggatg cgggccagcc gatccgcgaa cttacccttg
     2941 ccgccggcac cgatccgctc tcccagggcc tgccggtgct gtgcgtggtg ctggcaggcg
     3001 ggctcaccac caatgccctg tggtgcggct atctcatctg gcgcaaccgc tcggccgggc
     3061 agttcctggc ggcgctgccg cagggcggcg cggtgccctc gctggcggcc aactggctgc
     3121 tggcggcgct gggcggcacg ctgtggtatg gccagttctt cttctacacc atgggcgaga
     3181 gccagatggg ccgctatggc ttttccagct ggacgctgca catggccagc atcatcctct
     3241 tctccaccct gtggggcttt gccctgaagg aatggcgcgg cgcctcggcg cccacgcggc
     3301 ggcgggtgtg ggcgggcatt ggcctgctgg tgggggcaac ggtagtgatc ggcgcgggca
     3361 acatgctggc cgccgggcaa tagcggccac ggcttgccgg cgccggcgcc cgccgatata
     3421 gccggctgag gggaattgtt tcacaccatg cacgccaccc aacgcgaaag cctgatcctc
     3481 gacgcgctgc gccccacagg ctttgtcagc tatcgcgatc tggaagccca gctggccgcc
     3541 tcgccggcca ccatccggcg cgatctggcg cggctggagg ccgaggggcg gatcacccgc
     3601 gtgcatggcg gcgccaagct ggccggcgat ccggcagagc ccccggcccc tgccgccccc
     3661 cgccagcaat tgctgggcac ccctttcgat ctggcgatca cccagaacct ggccgccaag
     3721 caggcgatcg gccgcgccgc tgccgccctg tgcgagccgg gcgagggcat catggtggat
     3781 ggcggcacca ccaccttgca gatgtgcccg catctggccg ggctcgatct gcaggtgctc
     3841 accaattcgc tgcatatcgc cggcgccctg ctgccgcagc cgggcacgcg catattgctg
     3901 ccctcgggcg cggtgttccg cgagcagaac atcatcctgg ccccggcggg cgaggattcc
     3961 atgccgcgct tccatgcgcc caagctgttc atgggggcgg ccgctgtggg cccgcgcggg
     4021 gtgatgcagc atgatgtgat cctggtagcc gccgaaaggc gcctgatgga ccgcgccgag
     4081 caagtgatcc tgctggtcga tgccagcaag ttcggctcct cctcgggcgg catcgtctgc
     4141 gcgctcgacg aggtggatat cctcatcacc gatgccgcca tcacccccga acaggccgcc
     4201 atggtggaag cggccggcgt gcgcctgatc atcgcctgag cgcggtgcag acaccagcgc
     4261 tgccactggc gcggctgccg ggccttgcgc aggcaattgc cttgcccccg gggtccgggc
     4321 tagatcatct gcatgagcct cttttcccgc cccgggcccg cccacggccc gctctaccgc
     4381 ctgatctggt ggccaaccat ggcgctgggg ggtttcatcg gcctttcggt gctggcaacg
     4441 ctggcctatc gggtggtgcc gccgccggta acgatcacca tgctggcaga ccccaacggc
     4501 atcgccaagc aatggacccc gctttccgag atcgaccgct cgatggtgga tgcggtgatc
     4561 gccggggaag atgcccgctt ttgcgagcac catggcttcg atcaggccgg catccagtat
     4621 gccctgcggc gcgacatgaa cggcggcaag ctgcgcggcg gctccaccat cagccagcag
     4681 acggcgaaga acaccttcct gtggcagggc tcgggctggg atcgctatct gcgcaaggcg
     4741 ctggaggcct ggttcaccgt gctggtcgag catctgtggg gcaagcggcg gatcatggag
     4801 gtctatctca acgtcgcgga aaccggcatc ggcacctatg gcgtggaagc cggcgcccag
     4861 cgctattatc gccattcggc cgcccatctc agccaggtgg aagccgcccg cattgccgcc
     4921 gccctgccca gccccaagaa gcgcgccgtg gtgggcgcca gcggcttcac ccgccgccac
     4981 ggcaacgcca tcgccgcgcg cgaggcggtg gtgcgcaccg gcgcgctcga cagctgcgtc
     5041 tatcgctagg tctatcgcta ggcgccaggg ggtgggcggc gcgcaaatga aaaggggcgc
     5101 catcgctggc gcccccttcc ctgtggcagc ggccccgatc agaaatcgaa gcgcacgccc
     5161 gcgcggatcg catagagcga cggcttgata taggcctgcg ccttgggcga ggagtagctc
     5221 gagtagaggt actggccgca ggcattgccg cccaccgcca cacaggccac cttcaccacc
     5281 gcggtggcgc cgatatcctg ataggtgccc cagtcgcggt tcagcatgtt gaggaagttc
     5341 tcgatcgaga acagcgccgt cagcttgccg cggcccaggc ggggcaccgg gatctgctgg
     5401 gcgaagttca ggtcgatctt gttgtaggaa ggccccgtca tcaggttctt cggcgcgatc
     5461 gccccctgat agttgtagag cttggtgccg ttcaccagct tttgcagatt ggccatggtg
     5521 ctggccgaat cgaacgtcac attgccgcag gtaaggcccg agcaattggc gaagttcggc
     5581 acatagagca gctgcgaggt ggtgttcagc accgtgccga acacgcccga gcggccgttg
     5641 gtggaattgg tggtatagtc gttcatggtg atcgaatagg gctggccgga acgcagctcc
     5701 cagttgatcc ccaggcgggt ttcattgtcg ccgaagaact tgtggtggaa gttgatgttg
     5761 gcacgcacga tcgagctgat ttcgaacgcc gagcggccat aggcaccgcc cgaattggca
     5821 tcatccgtcg gcaccgaata ggagctggaa acggttgagc tgctcagcgc gccggcaatg
     5881 tcatgcaccc gggtgtgggt atagctggcc gaaacgtcga agtccttcca gctcttggtg
     5941 gcaccaatgg caaacacccg gctgatgccg cgcttggtgt tggtcagctc catgtcatag
     6001 ttgctgcccg gctgcaccgc cccattggcg gtgttcagcg tgccgccata gcgggtgcgg
     6061 ccatcgggcg tggtctgcac cacgccgttc accaccagcg gctgggcgcg cagatccacc
     6121 cagcgcacac ccgattgcgt gtcggagaac agcacgtcgg tgcggaaggt ccagccatcg
     6181 cccaggccca gcttggtgaa gtccgggtgc cagctcagcg tgatgttata cttccactgc
     6241 gccggcagct tgaacttggg atcgaggaag ttggtgtagg tattggccag taccgaggta
     6301 ttgtgcgcgg catagttcag cacgtccgag gggatcgtcg cgccgttcac attgctcagc
     6361 gcatcggcgc agatcgaggc gccgttccag gtggggttca ccgtcccggt cgagcggtcg
     6421 atacagctgc cattggccag gcgcacgata tcgacgctgg tgatggcagc gccgctgccg
     6481 ttgatgccct gcggcgcgcc gctgttcgaa tagttgttcg agatgaacac atccgacagg
     6541 ccgcccgcaa acaggcccag gccaccggcc accttcagcg tgtgggtggg ctgccagttg
     6601 aagccgatgc gcggctcgaa cttgtggcgg ccatccagcg tggcggtatt cgtcaggccc
     6661 ggatagagca ccgaatagcg cgcggtgaag ttggcgttct gggcgatagt gttgtcggcg
     6721 gtataccagt cgtagcggaa gccgccgttg atggtgaggt tgctctgcgc cttccacgta
     6781 tcctggaagc ccaccgtgtt ggtgtgatag gcccagctca caaagccatc gttcttgttg
     6841 ccggtggtgg tggtggtcag cgccagctcg ttggcctgct tggcggcaaa atcggccatc
     6901 gaatcgaagt agtaaagccc gttggggcca cccgagccag ccaggcccga accgccatag
     6961 acgaacaggt tatacagcgt ggagtagttg tggtcatact ccagcttgaa cgtgtgggcg
     7021 cccgcgcgat agctgaggtt gccctgatag gtgtagatgc ggttgcggaa cttgttcgcc
     7081 tggcgggcag tgtccggccc catcctcacc gtcggcgagc cgttcgagca ggtcgtctca
     7141 ttcgcatatt gcgtgccgct ggcggccgct gccagcgacg agctggtcgg gtccatacag
     7201 acgttcatct gcgcatagtc gggcccgttg taggcatcct gaccgcggcg ataatatttg
     7261 tacgccacgc gcacttcgct cgacaggcga tcggtccact tgccgttcca ctggcccgag
     7321 aaggcatcgg tgtgctcggt cagcttgtaa aaatcagaca gcagcagaat gtacggcgat
     7381 gtcgcgctgt tgctgccgcc gaccccggaa tagaagctgg gcagcacgtt ctcgtggtga
     7441 atatacgaca ccgacacgcg gtgcccttcg gtcacgttcc agtcgagctt ggcggcatat
     7501 ttctcgtcca cctcgttgat cgaggtgggc acggtgccaa tgttgtagtt gccgtaatat
     7561 ttgttgaagg tgttatacag cgtggtcagc cccggcatca cattaccaaa ggtgcccgtg
     7621 cccggatcga caaactggtt ggtggggttg gcagcgttcc acgcgatcac gttctggcca
     7681 tagggatagt agttcgagcc gttgagatag gtgcccgagg gcaccgtgaa gtcacccgtg
     7741 tgcaggttgg tcaccgcatt gctggcggtg ccatccatcg ggccccacag gtccggcgtg
     7801 ccttcggtca gccgctcgta ggcagcggcg aagaacagct tgtccttgat gatcgggccc
     7861 gagatgctgc cgccgaaatt ggtgaagggc aggatcttgg tgggcagcac cgaagcgccg
     7921 ctgccgggca ccaccagcgt gtcggcggtc ttggtgccgt tgaggcccgg gccgccgaaa
     7981 ttgccgaaag cggtcatgtg gaacttgttg ccgcccgact tgagcaccac gttcaccgcg
     8041 ccgccctgga agttgccttc cgaaatgtcg aagggggcgg ccttcacgct cagctggtcg
     8101 atcatttcca gcgagacgat cccgcgcgac gacggcaggc cgccgtagtt caggccgaag
     8161 ctgtcctgca tctgcacgcc atcgaccgag aagcgctggg tgcggatgtt gccgccggcg
     8221 atggacacgc cgccggcatt ggggttgaag cccgagagca ggtcgcggcg catcacgtcg
     8281 cgcacgtcgc ggcgggccga gacgatgtcc tggatgtcct tcgcccggaa ggaggtttcc
     8341 gaaccggtga tcagctggcg gccgccgcgc gcctgcaggc cggccacgat gatctgcttc
     8401 tgcatgatca ccggcagcga gaaggtgtcg cccagcgtca gcatcacatt gtcgtagatc
     8461 tgggtgggct ggccggccgc ttccaccgtc acggtgtagg gcccgcccac gcgcaggccg
     8521 cgcatggcga aattgccagt gccatcggtg gtcaccgtct cgcgcgtgcc ggtgggcgtg
     8581 tgcaggatgg taaccttggc gccgccaacc ggcgcgccgc tttcagcggt aacctggccg
     8641 gcgatggcag aagtggtgtc ctgcgcatag gcaggctggg caaccactgc ggcaatcgca
     8701 atggtgctgg cggccagggc cgctagagtc gaatatcgca tgatttatgc ccccgcattc
     8761 atcaagtcgg ctcgatgtgt atgtcgcacc cgggacgagc cacaccccca tgtgacgcaa
     8821 tgttagcgcg acacacccct tggcctcgct gcgttgcact atctttacag acaggaaagc
     8881 atatgcgccg acaccccgta acgagacgca aataggcagg aaatgcggca gtgcagcata
     8941 accgccacag tggtctgtgc tcttgcccga tggcggcact tgtgtatgcc cttggctgcc
     9001 ccaccctggc ggcatgaagg acagatccgt gcaggcgcat tcccattcca ccgcccatga
     9061 tcacgggcac gggcacactc acagccacga ccatgcccac gggcacgggc acgggcatca
     9121 ccatggccat caccatcatc atggcgatac gcatggcacc gcctttgccg tggcggtcac
     9181 gctcaacctg gcctttctgg tggtggaaac gggcgcgggc atctggggcc attcgatggc
     9241 gctgctggcc gatgccgggc acaatttctc ggatgtgctc tcgctgctgc tgtcgtgggg
     9301 ggccagcgcg ctggcccggc gcccgccttc ggcgcgctat acctatgggc tgaaaagctc
     9361 ctcgatcctg gcggcgctgg ccaatgcggc gctgctgtgg gtggcgctgg gggccatcct
     9421 catcgaaacg ctgcgccgcc tgttcgagcc ggccagcgcc gagggcacgg tgatgatctg
     9481 ggtggcggcg gccggcattc tggtgaacgg cctttccgct gtcctcttcg cccgcggcag
     9541 caaggaagat ctgaacctgc gcgccgcctt ccagcatctg ctggccgatg cggcggtgtc
     9601 agccggcgtg gtggtggcag gcatcgcggt agccctttcg ggctggagct ggatcgatcc
     9661 tgccaccagc cttgccatca ccgcgctgat cggctggggc agctgggggc tgctgcgcga
     9721 atcggtgcag ctgggcctgc tggcagtgcc ggccggcatc aacgaaagcg ccgtgcgcgc
     9781 agagctggcg gccaagcccg gggtgaccgc cgtgcatgac ctgcacatct ggccgatgag
     9841 cacgaccgag accgcgctca ccgcccatct cgtcatgccc gccggccatc cgggggatga
     9901 atggctgcat ggcgtggccc atgcgctgga acatgagcac ggcatcggcc atgtgacgct
     9961 gcaggtggaa acggaagaag ggcacgaatg cgcccttcat tccgacgggg tggtttaacc
    10021 ctcgggcagg gcggcagcaa agcgcagcgg ctcgccgatg gcggcagccg ccagcgcgcc
    10081 ttcccacacc acctgatggc cacgcacgat cgtgcccatc acccggccct tcagctccat
    10141 gccggtgtag ggcgaccagc cgcagcggct cgccagccat tcctcgctga cggtgaagcg
    10201 gcccttcaga tcgaccacgg tgaaatcggc atcatagccg gccgcgatcc gccccttgcc
    10261 ctgcaggcca aagaggcgct gcacgccggc gctggtcatg tcgatcagcc gggccagggt
    10321 catccgcccg ttcagcacat ggtccagcat cagcggcagc agggtctgca cgccgggcat
    10381 gccgctgggg ctggcgggat agggcttggc cttctcttcc agcgtgtgcg gcgcatgatc
    10441 gctgccgatc acatcgggca cgccctggcg cagccagtgc cacagcccat cgacatgcgc
    10501 gccgctgcga atcggcgggt tcatctgggc aagggtgccc agccgcggat aggcctcctc
    10561 gccgcgcagg gtgaggtgct ggggcgtcac ctcgcagctg gcgatatcgc ggtgctggct
    10621 gatcagctcc agctcggctg gcgtcgtcac atgcaggatg tggatcggcc ggcgggccgc
    10681 gcgcgccagc ttgaggatgc ggcgggtggc cagcagcgcg ctttcatcat cgcgccacac
    10741 cggatggctg gaaggatcgc cctccaccct gaaatccttg cgggcattca tccgcgcctc
    10801 gtcctcggca tggatcgcca cgcggcgcac gccgctggcc agcacgcggg caagctccgc
    10861 atcctcggcc accagcaggt tgccggtgga agcgcccatg aagatcttga cgccagcggt
    10921 gccgggaatg cgctccagct cggccagatg ggcggcattg gcggtggtgg cgcccacata
    10981 gaaggcatga tcgcaccaca tgcggtggtg ggcgcgggcc agcttgtcgt gcacggcctc
    11041 ggcggtatcg gtgttagggt tggtgttggg catctcgaac acggcggtaa tgccgcccaa
    11101 caccgccgcg cggctgccgc tttcgaggtc ttccttgtgg acaaggccgg gctcgcggaa
    11161 atgcacctgg ctgtcgatca cgccgggcag gatgtcgagc ccggtgcagt cgatccgccg
    11221 ggccgcatcg ggaaagtgcc ccagcccgac aattttgccg ccgctaacgg caacatcgca
    11281 gttttcgggg ccgttgggcg tgtggacggt gccgcctgtc agcaccagtt cgattgcttg
    11341 ggtcatcgcc cagccatgcc acaaaccggc cgcaggttta agccggaatg atgcgcggcc
    11401 tgacatccag cccgacgccg ggcttgacgc cggcgcccca gcccccatct tgcaggccat
    11461 gaccacgctt gccgcccgcg cgctgatccg ccttgccccc caggcccccg aggaaaaccc
    11521 cgccaccttc ctgcaggggc tggtgacgaa cgacgtggtg aaaggcccgc tgccggccta
    11581 tgccgcgctg ctgagcccgc agggcaaggt gctgttcgat ttcctcgtct gggccgatgg
    11641 cgccgacctg ttgatcgatt gcgcggccga cagtgccgag gcgctggcca aacgcctgtc
    11701 gctctatcgc ctgcgccggg ccattgccat cacccgcgag acggcgctgg ccgtgcactg
    11761 gagcgccagc gaagtgcccg gcgccgtgcc cgatccgcgc ctggcagcgc tgggctggcg
    11821 ctggctggcc cccgccgatg cagccccggc cactgcagcc cccgccgatg ccgcctggct
    11881 ggcccatcgc ctggcccagg gcgtggccga aggggcagag ctggccgata cgctgtggct
    11941 ggaatgcaat gcggtggagc tgaacggcgt ttccttcacc aagggctgct atgtggggca
    12001 ggagaacacc gcgcggatga actggcggca gaaggtgaac cgccggctgg tggtggtgcc
    12061 gctcgagcgt tccacccccg cccgccagcg cgccgcctac cccgcgctgg ggctggcggt
    12121 ggaccatctg ccgctggcgg cgatcacgcc ggatctggca ccgacgtggc tcgcactggc
    12181 cgaggcggag gagacagacc cagccgcgcc gtctcactga tcagcaggcg ctcggccata
    12241 tcccgcgcgg tggcgcgcgg catggccagc gctgcggcca gcggcgcgcc catcaacgca
    12301 tcgcccagcg ccagcagcac cagcgtcaaa gtcacatgcc gggtcgcttc cgagccgaag
    12361 gggtgcagtt cctccaccat ctggtgcacc gcatcgacga tcggcgccag cgcctcttca
    12421 ttgccggtga tcctcagcca gctggccagt tgcccgccgc cctcatgctc gaaggcatcg
    12481 aaggccagat ccaccaccgc ccgcgccgtg cccacgccca gccggctggc aatcaccgca
    12541 gagccgatcg ccgcgcagat attgtcggcc atataggcgg ccagcgcctt ctgcagttcc
    12601 gcggcagagc cgaaatgatg cagcagattg gcatgggtgc gccccatgcg cgccgccaca
    12661 gacttcagcg tgacagcctg cgcgccctcc tctatcagga tcaggcgggc ggcggccaac
    12721 gccgcggcgc ggctttcatc ggacgacaaa cgccggcgca aagtcatgaa aagtaatgcc
    12781 gaggcatgtt ttcggcctcc cctgtccctt ttgaagacaa ggcctttagg caattcgtcc
    12841 catttctgca agaacagcgg caaatgttga aacacaatga aataaacatc cgcaatatcc
    12901 gctttgccag tgcccctgcg gcgcgctggt ggctggcggg cgatccggtg gccacggcct
    12961 ggcacaatgc gctgtcggcc agtttcccgc gcggcgaggc ctatttcatc gaggccgtgc
    13021 gcgccaaccg cgccggcacc accggcgccc ttgcccggca gatagacgcc ttcatcgccc
    13081 aggaagccaa ccacagccgc gagcatcacc gcttcaaccg ccacctggcc gaagccggct
    13141 acgacatggc cgggatcgac gccgcgctgg ccgccttcat ggcggaaaac cgctcgcaat
    13201 gggatgtggt gaacctggcc gccaccatcg cgcttgaaca tttcaccgcg atcatcgcgc
    13261 atgatctgct ggcccggccc gattaccttg ccggggccga accggaaatg gcccgcctgt
    13321 ggcgctggca cgcggtggag gagatcgagc acaaggcggt ggccttcgac acctggctgc
    13381 atgccacccg ccgctggagc ggatggcagc gctggcgcct gcgctgcctg gtcatgctga
    13441 aggtctcgcg caatttcctc acccgccgca gcgccgatgc gctgtgcctg ctggcgcagg
    13501 atggcatcac cggctggcgg gcccgggcgc ggctggcggc ctatctgctg ggaaagcccg
    13561 gggttatccg ccgcatcctg ccagtctggt gcggcttctt ccgccccggc ttccacccct
    13621 ggcaaaccga cgaccgcgcc ctgatcgcgg ccgttgatct gcaagcaggg gtttaaccgg
    13681 caggctcagg ccgcgcggcg agtgggcgcg ccgcccagat cgtcgtcccc gccccaatca
    13741 tccccgcccc aatcatcgcc ctcccggtcc tcgtcaccgg caaggcccgt cagcgccggc
    13801 aggtccagcg cgcagcgcac cagcgtagag cgcaggcccg gccccatctc ggccggatgg
    13861 cccgcggcca ccagcgccat ctcgcccgtg gggcgaaagc ccagcttgcg cagcaggccg
    13921 cccgaggcga catcctccac cgcatggcca gcctgcagcc gccgatagcc cagcaaccgc
    13981 gccagcggca gcagcgcgcc ggccgcctcg gcgccaaggc cctgcccgcg ccaggccggc
    14041 gccacgaaga tctggaattc ggcttcaccg cgccgggcgc cggcgcgttc gcccggccgg
    14101 cggaacaggc cgatcgcccc caccgtctcg acaccgccgc catgcgggcg ggtgatcagc
    14161 aattgcggcg cgcgggcttc ctgccaggca aacacagaag ccgcttccgg ctctgccccc
    14221 cacagacccg ccgccagccg cccggttgcc cccggatcgg ccagcatggc gctcagatcc
    14281 ttccagtcct ctgcccagat aggccgcaag aacagccgct cggttcgggt aaacatggta
    14341 tggctctcca ctcaggggcc cccgttggcg cacatttata acgcaacgga gacaccgcgg
    14401 ttacgcattg cggcgaaacg ggccaaacgg cagatcatcc gttcaggatg gatttgccct
    14461 ggccgtaact gccgcaacag gctcgcgagg gagacgcaaa aagggagacg ggccgggccc
    14521 ctctccctcg aacggccagc gcttgcgggc atcatgccca aggcgccggc ggggccatcc
    14581 cgttggatgg cccatgatgt gccatccggc ttattcggcg gcttctgcca ccacgtcgac
    14641 cgacacatat ttgcggccga gcttgccatc gtggaaacgc acgcggcctt cggccagcga
    14701 gaacagggtg tggtccttgc ccatgcccac attcacgccc ggatacacgc gagtgccgcg
    14761 ctggcggatg atgatgttgc cgccgatcac ttcctgaccg ccgaacttct tcacgccaag
    14821 gcgcttggac tgcgaatcgc ggccgttacg cgaagagccg ccagctttct tatgtgccat
    14881 ctgtcaaact cctcgagaaa atcaggcggc cgcgaccgac aggatgcgca gcagggtgag
    14941 ctgctggcgg tggccgttgc ggcggcgata gttgtggcga cggcgcttct tgaagacgat
    15001 caccttctcg ctcttggcct gggcgatgat ctcggccgac accgtcacct tcgaggcgtc
    15061 ggcgatctgg ccgtcttcgc cggccagcag aatgtcagcc agagtgatgg tctcgccggc
    15121 ttcgcccgcc agcttctcga ccgcgatctt gtctccggcg gcgacccgat actgcttgcc
    15181 gcccgtgcgc acgactgcga acatgatacc tatccaattc caaattgcgt ggccgccccg
    15241 gccgcgatgc cccgccacgc ccgcctgatg acgaaccctg gcaagagcgg ccgcggaacg
    15301 gcgcgcgaag ctgaccgggc gaaatgcccg agccctcttt cgaatgaagc ggtgccgtta
    15361 gtgtagttgg caggcagagt caatgccgtg tctgcggcaa ggccatgccg ccggccctgc
    15421 catccgcccc ccggcaggcc gcacgcagcc gcgctttatc gcctctggcg ccggcgccca
    15481 gccgtgctat ggcctcttgc catgcacaca cgctgccaca tcgccgcccc gcttgccaag
    15541 gcgccccagg ccgggggccc ggcccccttg cgaatcggcg ccgcgctggc gctggccggg
    15601 ctgctggcgg gctgcagcac gggcggctat ccctcgctgc gcccacgcgc ggaagagcag
    15661 gcctcctatc gcaccacccc gcccgaggcg ccccgcccgc ccgaagcgct gtcggccgat
    15721 ctgggccagc agctggccag gctggtggaa gaggcccggc agggccacgc cgccttcctg
    15781 gcccggctgc cccatgccga agcggcgctg gcggccaatg gcgattcgcc ggccggcacc
    15841 agcagctggt cggaagcgaa tgaggcgctg gccgagctgg acaccgcccg cgcgcccgcc
    15901 ggtcaggcgc tggccgccat cacccgcctc tatgtggacg accggctgga ccacgcgctg
    15961 gacgatgccg ccgccggcaa tggcctggcc cgcccggccg gcgcccggct ggatgccgcc
    16021 caggcccagg tggccgccct ggtggccgag gaagacggca agctggccgc cctgcaggcc
    16081 cgcttgcccg gttgaaacgg cccggctgag gttgggatca gccgcgcgcc tgcccccagc
    16141 gcgccagatc cgccgcatcc tcggcgcgcg gctcgatcca gtgccactgg ccggcgcggc
    16201 gctcgcgctt ccagaaccag ctgtccgatt tgaggtggtc catggcaaaa tccgccgcct
    16261 cgaaagcggc gcggcggtgg cgggcagcgg cggccaccag cacgatcggc tcgcccggcc
    16321 gcatcacccc gctgcggtgg acgatcagca gccccgccag atcccagcgc gccagcacat
    16381 cggccgccag cgcttccatg ccgggcaggg tcagcggcgc gtaatgctgc agctccagcg
    16441 cttccacccc ctcgcccgcg cgcacctggc cgatgaagct ggcaatgccg ccggctgcct
    16501 catgcgcggc ggcaaacccg ccaagcgccg tggccggatc gaaagccgcc agcgccagcc
    16561 gcacatcggc ccgcccgctc atccgccaga caccggcggc aggaaggcca gctcgtcgcc
    16621 ttcggccaat accaggccaa ccggcgccac cagcgcgcca ttcagcgcca ggcggatgcg
    16681 cggggccagc agcgcctctg ccagcgccgg ggcaaggcct gccagcacat cggccagcgg
    16741 gcccggcagc acggcctgct cgcccacgcc ggccagatcc gccagccggc ccagaaacac
    16801 cagccgtgca gcgcccccca tcgctcagcc cccggtcatc gacatgtggc gggccagtgc
    16861 cggcgcggcg cccgcccggt ctatggcgaa atggtggcgg gccggcttca tgcgcatcgc
    16921 ctcgtccagc gcctcgtcca gcgccgcctc gggcccggcg ctgcgcagcg ccgcgcgcag
    16981 atccacctgc tcgcgcccgc caaggcaggc aaacagctgg ccggtcgcgg tcacccgcac
    17041 gcggttgcag ccctcgcaga aattgtcggt cagcggggtg atgaagccca gccgcccgcc
    17101 ggtctcggcc agccgcacat agcgggccgg gccgccgctg cggtgcccat cggcctccag
    17161 cgtgaaggcc tgcgccagct gcgcccgcac ggccgacagc ggcaggtaat gatcgaagcg
    17221 gtcttcctcc acctcgccca gcggcatcac ctcgatcagc gtgatctcat ggcccgcgcc
    17281 atgcgcccag cgcaccagat cggggatttc cgcctcgttc acgcccttca gcgccaccgt
    17341 gttcagcttc accgccagcc cggccgcccg tgccgcggca atcccctcca gcacggcggg
    17401 cagctggtcg cgccgggcga tgcgggcaaa cacctccctg tcccgcgtgt cgagcgagac
    17461 attcacccgc ctgaccccgg ccgcatacag cgcctcggca tgggcggcga ggtgggtgcc
    17521 attggtggtc agcgtcagct cgtccagccc ctggcccagc tggcggccca gcgcgcgcac
    17581 cagctcgatc atgtcgcg
//
LOCUS       DASRYE010000262         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_157446,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000262 DASRYE010000000
VERSION     DASRYE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157446"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1366
                     /locus_tag="VFF98_13575"
     CDS             23..1366
                     /locus_tag="VFF98_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64707.1"
                     /translation="MDLITSPAEPLSGRDPGLLRGDPISGERYFAPDFARKEWDMLWT
                     RIWHIAGRTAEIPEAGDYLVHDFMKESVICIRQEDGTIKAFYNSCRHRGMRLAAHTSS
                     ADTIACPYHGWRFAKDGTLVHAQDRHDFPQGDPCGKLKLRELRCDTWGGFVWYTMAPE
                     GPGLAEYLAPMPEVYKGYPMDTAVRVAWYRIELNANWKFVTDNFSESYHTRTAHPQVP
                     PWIDQDVDTARHEIWPTGHGRTVQPMRPSLSDRLPADQPHPYKMMLAAWGIDPDGYAS
                     YEEMAMQGWLDLKAAKRAQWREKGYLHYEHMDDEEITDSPHTVIFPNVTISFLPDNII
                     LFRSEPHPTDPEKCYFDLWCMAFPVAGQTHVESIMAGMRPLREVAECEHRRFDGGRGV
                     PELAGQIVYQDMELAEGMQAGMHSRGYTDAYLPAQESRVRFFHEVLNDWLEGRRP"
     gene            1366..2052
                     /locus_tag="VFF98_13580"
     CDS             1366..2052
                     /locus_tag="VFF98_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64708.1"
                     /translation="MANVAITGAGRGIGLELVRQHLASGDRVWALVRNPEGAGDLAAL
                     AAGSGGQLSVHQLDVADDASVKAGAASTGSDRVDILYNVAGVAGPAESELESADWAAW
                     DEAFRIMVQGPLRVLQAFLPRLGAGSKVINFSSQLAASTWPYGGFYAYGAAKAGLNRM
                     MRSVAIDLKDRGIVVGSIHPGWVQTDMGGPGAEITPQESAAGIIKLARGWPLEHTGDF
                     YKWNGEPHAW"
     gene            2063..2497
                     /locus_tag="VFF98_13585"
     CDS             2063..2497
                     /locus_tag="VFF98_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU64709.1"
                     /translation="MPYTGPLEDRILIRELIETYAHGVMTRDAALWGGTWAEDAFWAL
                     PEWPDLEGFSGKAAIVAGWAESMKVYGIDGCRKPMIYVATPGSIEVSGAQAKAVCYTS
                     EIFDDPATGETKRVRGRYDDELAKVDGRWLFTRREYRIMHLA"
     gene            complement(2541..2900)
                     /locus_tag="VFF98_13590"
     CDS             complement(2541..2900)
                     /locus_tag="VFF98_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HZU64710.1"
                     /translation="MSLTHITLLKRRPGMSKADFITYYEACHARIGEQVLAGYATRYE
                     RRFLFPLDGEDNLHDPDVVMEIDFPDAATRDACFAAMAAPDIMAMILADEEKLFDRSR
                     NRSFWVEGHRSVLPPVG"
     gene            complement(2897..3688)
                     /locus_tag="VFF98_13595"
     CDS             complement(2897..3688)
                     /locus_tag="VFF98_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU64711.1"
                     /translation="MNPISLASGVVPEFGPVETIIAAREGGFDAVGLWVEPASWTAET
                     TRAARTALADAGLPLLDVEVVWLRPDTRLDDHRRLIDIGAELGARNVLCVSSQPDAAT
                     TIAELTALCRHAEGTGLRVALEFGIFTEVKNLRAASAILDAVAHPLRALLIDPIHVDR
                     SGTTLAEIAAVDPALLPYAQFCDALAERPDPADFDAIIHDAIDLRQQCGEGALPLAAL
                     LQALPADIPLAIELRSAALRSAWPDPCERARAVATATRAWLETQA"
     gene            complement(3751..4929)
                     /locus_tag="VFF98_13600"
     CDS             complement(3751..4929)
                     /locus_tag="VFF98_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU64712.1"
                     /translation="MDHASRIGDAAEIMLDYVEQNKTFLTDKTMRVPSASYTDPAQWE
                     AEMAQVFRKVPLMLAFTSEMPNPGDYKAMEAIGLPILITRGKDGVARAFLNVCSHRAA
                     PVANEGHGNCARFTCKYHGWTYGPDGKLIGISEASKFGEVDKASLGLKALPCKEQAGM
                     IFVGLTPGMELDLDAYYRGFLEDFEALDFANWSYMGHRVIEGANWKIAYDGYLEGYHF
                     ASLHPQTIFPRTPSNCMHFEGFGPNFRIGFPQRRISEALRPLPREEWGRQENNGFDFV
                     RIFFPNVSIFVAPEITQVAQLFPGPTPDRNRTVLNYLRREPIRDEEDRAAVEAYMNFF
                     RDVTYTEDYLIGLEIQKGLESGAHKEMTFGRNERGNQYFHEWLNWYMAGDANAPEPQM
                     "
BASE COUNT          904 a         1623 c         1755 g          889 t
ORIGIN      
        1 aggcggggcg gggagattga cgatggacct catcaccagc ccggccgagc cgctgagcgg
       61 ccgcgatccg ggcctgttgc gcggcgaccc gatcagcggc gagcgctatt ttgcgccgga
      121 tttcgcccgg aaggaatggg acatgctgtg gacgcggatc tggcacattg ccggccgcac
      181 tgccgaaatc cccgaggcgg gcgattatct cgtccatgat ttcatgaagg aatcggtgat
      241 ctgcatccgc caggaggatg gcacgatcaa ggctttctac aattcctgcc ggcaccgggg
      301 catgcggctg gccgcgcaca cctcatcggc cgacaccatc gcctgcccct atcacggctg
      361 gcgctttgcc aaggatggca cgctggtcca cgcgcaggac cggcatgatt tcccccaggg
      421 cgacccctgc ggcaagctga agctgcgcga actgcgctgc gacacatggg gcggcttcgt
      481 gtggtatacg atggcgcccg aaggccccgg ccttgccgaa tatctcgcgc ccatgcccga
      541 ggtctacaag ggctatccga tggatacggc ggtgcgcgtg gcgtggtatc gcatcgagct
      601 caacgccaac tggaaattcg tcaccgacaa tttctcggaa agctatcaca cccgcaccgc
      661 ccacccgcag gtgccgccgt ggatcgacca ggatgtcgat accgcccgcc acgagatctg
      721 gcccacaggg catggccgca ccgtgcagcc gatgcgcccc tcgctgtcgg atcggctgcc
      781 ggccgatcag ccgcacccct acaagatgat gctggccgcc tggggcatcg atccggatgg
      841 ctatgcctct tacgaggaga tggcgatgca gggctggctg gatctcaagg cggccaagcg
      901 ggcgcagtgg cgcgagaagg gctatctcca ctacgagcac atggacgacg aggagatcac
      961 cgacagcccc cacacggtga tcttccccaa tgtgacgatc agcttcctgc ccgacaacat
     1021 catcctcttc cgctccgagc cgcatccgac agacccggag aaatgctatt tcgacctgtg
     1081 gtgcatggcc ttcccggtgg ccgggcaaac ccatgtggaa tcgatcatgg ccggcatgcg
     1141 ccctctgcgc gaagtggccg aatgcgaaca ccgccgcttt gatggcgggc gcggcgttcc
     1201 cgaactggcc ggacagatag tttaccagga tatggaactg gccgaaggca tgcaggcggg
     1261 catgcattcc cgcggatata cagatgccta tctgccggcg caggaaagcc gggtgcgctt
     1321 cttccacgaa gtgctcaacg attggctgga gggaaggaga ccttgatggc aaatgtggca
     1381 atcacgggtg ccgggcgcgg catcgggctg gaactggtgc gccagcacct ggcctctggc
     1441 gatcgcgtgt gggcgctggt gcgcaacccc gaaggcgcgg gcgatctggc agcgctggcg
     1501 gccggatcgg gcggtcagct cagcgtgcac cagctcgatg tggccgatga cgcctcggtg
     1561 aaggccggcg cggccagcac cggcagcgac agggtggata tcctctacaa tgtggcaggg
     1621 gttgccggcc ctgccgaaag cgagctggaa agcgccgact gggccgcctg ggacgaggcc
     1681 ttccgcatca tggtgcaggg gcccttgcgc gtgctgcagg cctttttgcc gcggctgggc
     1741 gcgggcagca aggtgatcaa cttctccagc cagctggccg cttccacctg gccctatggc
     1801 ggcttctatg cctatggcgc ggccaaggcg gggcttaacc ggatgatgcg ctcggtggcg
     1861 atcgacctga aggacagggg catcgtcgtc ggcagcatcc accccggctg ggtgcagacc
     1921 gacatgggcg gccccggcgc cgagatcacc ccgcaggaga gcgctgccgg catcatcaag
     1981 ctggcccgtg gctggccgct ggaacacacc ggcgatttct acaaatggaa cggcgaaccc
     2041 cacgcctggt aaggagaagc ccatgcccta taccgggccg ctggaggatc gcatcctgat
     2101 ccgcgagctg atcgagacct atgcccatgg tgtgatgacg cgcgatgcgg ccctgtgggg
     2161 cggcacctgg gccgaggacg ctttctgggc cctgccggaa tggccggatc tggaaggttt
     2221 ttccggcaag gcggcgattg tcgccggctg ggcggaatcg atgaaggtct atgggatcga
     2281 tggctgccgc aagccgatga tctatgtcgc cacgcccggc agcatcgagg tgtcgggcgc
     2341 gcaggcgaag gcggtgtgct acacctccga gatcttcgat gaccccgcca ccggcgagac
     2401 caagcgcgtg cgcggccgtt atgacgacga gctggccaag gtggatggcc gctggctgtt
     2461 cacccggcgc gaataccgga tcatgcatct ggcatgaggg ggagagcccg gcggcgccag
     2521 cgctgccggg ctttttcgca tcaccccaca ggtggcagca cggagcggtg cccttccacc
     2581 caaaagctgc ggttgcggct gcgatcgaac agcttttcct cgtcggccag gatcatcgcc
     2641 atgatgtcgg gcgcggccat ggcggcaaag caggcatcgc gcgtggcggc atcgggaaaa
     2701 tcgatctcca tcaccacatc gggatcgtga aggttgtcct cgccatccag cgggaacagg
     2761 aagcggcgct catagcgggt ggcatagccg gccagcacct gctcgccgat gcgggcgtgg
     2821 caggcttcgt aataggtgat gaaatcggcc ttgctcatgc ccgggcggcg cttcagcagg
     2881 gtgatgtggg tgaggctcat gcctgtgtct ccagccaggc gcgagtggcg gtggccacgg
     2941 cgcgggcgcg ttcgcaagga tccggccagg cgctgcgcag cgcggccgag cgcagctcta
     3001 tcgccagtgg aatgtcggcc ggcagggcct gcagcagggc cgccagcggc agggcccctt
     3061 cgccgcattg ctggcgcagg tctatcgcat catggatgat ggcatcgaaa tcggccggat
     3121 caggccgctc tgccagcgca tcgcagaatt gcgcataggg cagcagggca ggatcgacag
     3181 cggcgatctc ggccagcgtg gtgcccgaac ggtcgacatg gatcggatcg atcagcagcg
     3241 cgcgcaaggg gtgggccacg gcatcgagaa tggcgctggc cgcccgcagg ttcttcacct
     3301 cggtgaagat gccgaattcc agcgccacgc gcaggcccgt gccctcggca tggcggcaca
     3361 gcgcggtcag ctcggcgatg gtggtggccg catccggctg cgaggagacg cacagcacat
     3421 tgcgcgcgcc cagttcggcg ccgatgtcga tcagccggcg gtgatcgtcc agcctggtgt
     3481 cgggccgcag ccacaccact tccacatcca gcagcggcag gccggcatcg gccagcgccg
     3541 tgcgggcggc gcgggtggtc tcggcggtcc agctggctgg ctccacccac aggccaacgg
     3601 catcgaaacc gccctcgcgc gcggcgatga tggtctccac cgggccaaat tcgggcacca
     3661 cgcccgaggc gagcgatatg gggttcaagc ctctctctcc cgaaaaggaa aagggcccgg
     3721 ccggtttctc cggcctgggc cctgcaggga tcacatctgc ggttcggggg cgttggcgtc
     3781 gccggccatg taccagttca gccattcgtg gaaatattgg ttaccacgct cgttgcggcc
     3841 gaaggtcatc tccttgtggg cgccgctttc cagtcctttc tggatttcca ggccgatgag
     3901 atagtcctcg gtgtaggtca cgtcgcggaa gaagttcata taggcctcaa cggccgcgcg
     3961 gtcttcctcg tcgcggatcg gctcgcggcg cagatagttc agcacggtgc ggttccggtc
     4021 gggcgtcggc ccggggaaca gctgggccac ctgggtgatt tccggcgcca cgaagatcga
     4081 cacgttgggg aagaagatcc gcacgaaatc gaagccgttg ttctcctgcc ggccccattc
     4141 ctcgcgcggc agcgggcgca gggcctcgga aatgcggcgc tgggggaagc cgatgcggaa
     4201 attgggcccg aagccttcga aatgcatgca gttggaaggg gtgcgcggga agatcgtctg
     4261 cgggtgcagc gaggcgaagt gatagccttc gagatagccg tcataggcga tcttccagtt
     4321 ggcgccctcg atcacgcggt ggcccatata ggaccagttg gcaaaatcga gcgcttcgaa
     4381 atcctcgagg aagccgcggt agtaggcgtc gagatccagc tccatgccgg gggtgaggcc
     4441 gacgaagatc atccccgcct gctccttgca gggcagcgct ttcaggccaa ggctggcctt
     4501 gtccacctcg ccgaacttgc tggcttcgga aatgccgatc agcttgccat cgggcccata
     4561 ggtccagccg tgatacttgc aggtgaagcg cgcgcaattg ccatgcccct cattggccac
     4621 aggcgccgcg cgatgcgagc acacgttgag gaaggcgcgg gccacgccat ccttgccgcg
     4681 ggtgatcagg atcggcaggc cgatcgcttc catcgccttg taatcgccgg gattgggcat
     4741 ttcggaagtg aaggccagca tcagcggcac cttccggaac acctgcgcca tctcggcttc
     4801 ccactgcgcc ggatcggtgt agctggcaga gggcacgcgc atcgtcttgt cggtgaggaa
     4861 ggttttgttc tgctcgacat aatcgagcat gatctcggcg gcatcgccga tgcggctggc
     4921 atgatccact gtattctccc gaatcttcta tgctgtgctg ggggcaatat acccttatct
     4981 atacgcctcg gcgccagtgg gcgggcctcg cattttggcc aattggccag ctgcgggcga
     5041 gggggcaggt gaagggcatg gagacgagga tttcgccccc ctgggcctgg ctgaccattc
     5101 ccccccgcca tcaggctgcc ttcaccggcg tctgggaggc ggccaccatt gccgatgcgg
     5161 ccttcgcgtt g
//
LOCUS       DASRYE010000263         5286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_30525,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000263 DASRYE010000000
VERSION     DASRYE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30525"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFF98_13605"
     CDS             complement(<1..547)
                     /locus_tag="VFF98_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZU64713.1"
                     /translation="MLYNAYELQRATLSGASAWASVMAEMLNNPMMPGSLFGGGAVMA
                     SALDVFAHAAMPRGKPAFDIETVDVDGAVHSVEEAIVAHRPFGNLLRFSHAGLPDNAP
                     RLLIVAPMSGHYATLLRGTVARMLERAVVYVTDWADAKMVPLSAGGFDLDDYIDYIIG
                     FLEHIGGESGGARPHMLAVCQP"
     gene            831..2690
                     /locus_tag="VFF98_13610"
     CDS             831..2690
                     /locus_tag="VFF98_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZU64714.1"
                     /translation="MPASTTPSPEPVLASVAPGAASDGAAAPAPAARRNLGPLKMIWL
                     AALAYPGRLAMAGGALTVTALTTASVPWGFKQVIDKGFTRGSDTSTIDHWFLMLLAMV
                     LVLAVGTAVRFYNVSWLGERVVADIRLAVQKNLLRLPPSFFEANSPKEISSRLTADTA
                     LIEQVVGTTVSVALRNVITAFVGLAILFWLAPQLTGALIVAVPLVVAPIIWFGRRLRK
                     ESRSSQDRVADIGAMVAEVLGAARIVQAFNQESREGERFAAAVERSFATARKRITLRS
                     ALTSVAMGLVMGSLTLLMWEGALDVAAGTISGGTIAQFVLTGALVAGAFGSLSEVYGD
                     LVRGAGAASRLAELLNEQPAIAPPARPLALPVPPRGGLSFQNVTFRYPARPEVAALAD
                     FSLTVEPGETVAIVGPSGAGKSTLFLLAERFYDPQVGAVRLDGVPLASVDPAEIRARI
                     ALVPQEGVLFAASARDNLRYGRWDASDEEIWEAARAANAETFLRALPEGLDTFLGEDG
                     GRLSGGQRQRMAIARAVLRDAPILLLDEATSALDAESERLVQEALERLMQGRTTLVIA
                     HRLATVRDADRIVVMDGGRLVEAGTHEGLSRAGGLYARLAALQFDDTALAAQH"
     gene            2732..3547
                     /locus_tag="VFF98_13615"
     CDS             2732..3547
                     /locus_tag="VFF98_13615"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZU64715.1"
                     /translation="MSLIVTAVLLGILEGLTEFLPVSSTGHLILATKFFGYDDDQWAV
                     FNIVIQLGAILAVVVLYWRTIWAVVEGLLRGEQAAIRFVVNLLVAFMPAAVIGVLLKK
                     HIEAQLANPLGVAWALVIGGFAILAIERLAKTGDYVGVGQLPVGKALGIGFMQCLAMF
                     PGVSRSGATIMGAMCMGVNRRTAAEFSFFLAIPTMLGATVLELGSHWKTLAHASTNVG
                     FGEIAVGFAVAFVVALAVIKGFVAYISRHGFSPFAIYRILAGGAALWLLTHGA"
     gene            3730..3897
                     /gene="rpmG"
                     /locus_tag="VFF98_13620"
     CDS             3730..3897
                     /gene="rpmG"
                     /locus_tag="VFF98_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZU64716.1"
                     /translation="MAKPATVKIRLVSTADTGFFYVTKKNPRNTTEKFTFKKYDPVVR
                     KHVEFKEAKIK"
     gene            3938..4603
                     /locus_tag="VFF98_13625"
     CDS             3938..4603
                     /locus_tag="VFF98_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZU64717.1"
                     /translation="MTQRMTIFRFFSVQGLLAMVPAALCASVPLAPAAYAQAPAPAGD
                     LDRAVAALRGIDTLRADFTQTDADGRQVKGVLSLKRGGKIRFQYQPGYPVLIIGDGRA
                     LTVVDTEMKQVQRWPVGDSPLGALLDPRKDVARFGRVVPGYDPRLLTVEVRDRGHPEY
                     GTLNLLFVRKAEAPGGLELAGWTTMDAQNRRTVIRLAGHHYGLALAEDLFRYTDLRAR
                     PHH"
     gene            4943..>5286
                     /locus_tag="VFF98_13630"
     CDS             4943..>5286
                     /locus_tag="VFF98_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZU64718.1"
                     /translation="MLSVATWNINSVRLRMPLVERFLAEEAPDVLCLQEIKTVAELFP
                     AEAFAALGYTYQAVHGQKGYHGVATVARVPLREVSRRDWQANGEARHVGVELPDHGIT
                     IENVYIPAGGDIP"
BASE COUNT          794 a         1750 c         1788 g          954 t
ORIGIN      
        1 tgggctggca cacggccagc atgtgcgggc gcgcgccgcc gctttcgccg ccgatatgtt
       61 cgagaaagcc gatgatataa tcgatgtaat cgtcaaggtc gaagccgccg gccgacagcg
      121 gcaccatctt ggcatcggcc cagtcggtca cgtaaaccac cgcgcgttcc agcatgcggg
      181 ccaccgtgcc gcgcagcagc gtggcataat ggccgctcat cggcgccacg atcagcaggc
      241 gcggggcatt atcgggaagg ccggcatggc tgaagcgcag caggttgcca aacgggcggt
      301 gcgccacgat cgcttcctcc acggaatgca ccgcgccatc cacgtccacc gtctcgatat
      361 cgaaggccgg cttgccgcgc ggcatggccg catgggcaaa cacatccagc gccgaggcca
      421 ttaccgcgcc gccgccgaac aggctgccgg gcatcatcgg attgttgagc atctcggcca
      481 tcaccgaggc ccaggcactg gcgccgctca gcgtggcgcg ttgcagttca taggcgttgt
      541 aaagcactgt cggcatcctg gattgggggc attttgcggg tggcttgcct ttatgcaaag
      601 gattgccaca aaccgccttg catggcgatg aatttgggta acggattcca ccgtattggc
      661 cgataggatg agagcaaatc gcttgcgctg caatgcagca tgcggaacat accggattta
      721 caggcagaat tccgctccat tgccgggcga atcagcgatt gccagccaat gccgcaaggc
      781 cgcgcgcgcc ctgttccctc gccccggaaa atcgactagt ggcgaaggcc atgcctgcca
      841 gcaccacgcc ctcgcctgaa cctgtcctcg cctcggtcgc gcctggcgct gcgtccgatg
      901 gggcggcggc ccccgcgcct gcggcccgcc gcaacctggg gccgctgaag atgatctggc
      961 tggccgcgct tgcctatccc gggcggctgg cgatggccgg cggcgcgctc accgtcaccg
     1021 cgctcaccac cgcttccgtg ccctggggct tcaagcaggt gatcgacaag ggcttcaccc
     1081 gcggcagcga cacttccacc atcgatcact ggttcctcat gctgctggcc atggtgctgg
     1141 tgctggcggt gggcacggcg gtgcggttct acaatgtcag ctggctgggc gagcgggtgg
     1201 tggccgatat ccgcctggcc gtgcagaaga acctgctgcg cctgcccccc agcttcttcg
     1261 aggcgaacag ccccaaggaa atctccagcc ggttgacggc cgataccgcg ctgatcgagc
     1321 aggtggtggg caccaccgtg tcggtggcgc tgcgcaatgt catcaccgct tttgtcgggc
     1381 tggcgattct cttttggctg gccccgcagc tcaccggcgc gctgatcgtg gcggtgccgc
     1441 tggtggtggc gccgatcatc tggtttggcc gccgcctgcg caaggaatcg cgctccagcc
     1501 aggatcgcgt ggccgatatc ggcgcgatgg tggccgaagt gctgggggcg gcgcgcatcg
     1561 tccaggcctt caaccaggaa tcgcgtgagg gcgagcgctt tgccgccgcc gtggagcgca
     1621 gctttgccac cgcgcgcaag cgcatcaccc tgcgctcggc gctgaccagc gtggcgatgg
     1681 ggctggtgat gggctcgctc accctgctga tgtgggaagg cgcgctggat gtggcggccg
     1741 gcaccatttc gggcggcacc attgctcagt tcgtgctgac cggcgcgctg gtggccggcg
     1801 ccttcggctc gctgtcggaa gtgtatggcg atctggtgcg cggcgccggt gctgccagcc
     1861 gcctggccga attgctgaac gagcagcccg ccatcgcccc gcctgcgcgc ccgctggcgc
     1921 tgccggtgcc gccgcgcggc gggctgtcgt tccagaatgt caccttccgc tatccggcgc
     1981 ggccggaagt ggcggccctt gccgatttca gcttgacggt ggagccgggc gagacggtgg
     2041 cgatcgtcgg cccctcgggc gcgggcaaga gcacgctgtt cctgctggcc gagcggttct
     2101 acgatccgca ggtgggcgcc gtgcgccttg atggcgtgcc gctggccagt gtcgatccgg
     2161 ccgagatccg cgcgcgcatc gccctggtgc cgcaggaggg cgtgctgttt gccgccagtg
     2221 cgcgagacaa tctgcgctat ggccgctggg atgccagcga cgaggagatc tgggaggcgg
     2281 cccgcgcggc caatgccgaa accttcctgc gcgcgctgcc cgaggggctg gacacgttcc
     2341 tgggcgagga tggcgggcgg ctttccggcg gccagcgcca gcgcatggcg attgcccgcg
     2401 ccgttctgcg cgatgccccg atcctgctgc tcgacgaggc gaccagcgcg ctggatgccg
     2461 agagcgagcg gctggtgcag gaagcgctgg agcggctgat gcaggggcgc accacgctcg
     2521 tcatcgccca tcgcctggcc accgtgcgcg atgccgaccg gattgtggta atggatggcg
     2581 gccggctggt ggaagcgggc acgcatgagg ggctgagccg ggccggcggc ctgtatgcgc
     2641 gcctggctgc cctccagttc gacgataccg cgctggccgc gcaacactaa agctttttcc
     2701 caacgccctt tctgaaaagg acgcctcttc catgtctttg atcgtgaccg ctgttctgct
     2761 cggcattctg gaggggctca ccgagttcct gcccgtctcg tcgaccggcc acctgatcct
     2821 ggccaccaaa ttcttcggct atgacgacga ccagtgggcg gtgttcaaca tcgtcatcca
     2881 gctgggggcg attctggcgg tggtggtgct ctattggcgc accatctggg cggtggtcga
     2941 agggctgctg cgcggcgaac aggccgccat ccgctttgtc gtcaacctgc tggtggcctt
     3001 catgccggcg gcggttatcg gcgtgctgct gaagaagcat atcgaggcgc agctggccaa
     3061 tccgctgggc gtggcctggg cgctggtgat cggcggcttt gccatccttg ccatcgagcg
     3121 gctggccaag accggcgatt atgtcggcgt gggccagttg ccggtgggca aggcgctggg
     3181 catcggcttc atgcagtgcc tggcgatgtt cccgggcgtg tcgcgttcgg gcgcgacgat
     3241 catgggcgcc atgtgcatgg gcgtgaaccg gcgcacggcg gccgaattca gcttcttcct
     3301 cgccattccc accatgctgg gcgccaccgt gctggaactg ggcagccact ggaagacact
     3361 ggcccatgcc agcaccaatg tcggcttcgg cgagatcgcc gtgggctttg cggtggcttt
     3421 cgtggtggcg ctggcggtga tcaagggctt tgtcgcctat atcagccgcc atggcttctc
     3481 gccctttgcc atctatcgca tcctggccgg cggcgccgcg ctgtggctgc tgacccacgg
     3541 agcctgagac gggggctgag gggccggccc cgctactccc tcttgactct tcagcggcgc
     3601 tgacgcaaag ggccgccttc aacgggcggc gcttttgcgg gcgccgccct ctcgcattct
     3661 atcaaccggt ggccagcccg ttcgcgcgaa caggccccgc cggatcggga cagattttag
     3721 gaaagcgcca tggcaaagcc cgcaaccgtc aagatccggc tggtttccac cgccgacacc
     3781 ggtttcttct acgtcaccaa gaagaacccg cgcaacacca ccgagaagtt caccttcaag
     3841 aagtatgacc cggtggtgcg caagcacgtc gagttcaagg aagccaagat caagtaattc
     3901 agcgcgggtt caagccccgg gcggcaacgc gggcggcatg acccaacgga tgacgatctt
     3961 tcgcttcttc agcgttcagg ggctgctggc catggtgccg gcggcccttt gcgcatctgt
     4021 gccgctggcc cctgccgcct atgcccaggc gcccgcgccg gccggcgatc tcgatcgcgc
     4081 ggtggcggcc ttgcgcggaa tagacaccct gcgcgccgat ttcacccaga ccgatgccga
     4141 tggccggcaa gtgaagggcg tgctcagcct gaagcgcggc ggcaagatcc gcttccagta
     4201 tcagccgggc tatccggtgc tgatcatcgg cgatggccgc gcgctgacgg tggtggacac
     4261 cgagatgaag caggtgcagc gctggccggt gggcgattcg ccgctgggcg cgctgcttga
     4321 tccgcgcaag gatgtggccc gctttggccg ggtggtgccc ggctatgatc cgcgcctgct
     4381 gacggtggag gtgcgcgaca ggggccaccc cgaatatggc acgctcaacc tgctgttcgt
     4441 ccgcaaggcc gaggcgccgg gcgggctgga gctggccggc tggaccacga tggacgcgca
     4501 aaaccgccgc acggtgattc gcctggccgg ccaccattat ggcctggccc tggccgagga
     4561 cctgttccgc tacaccgatc tgcgcgcccg gccgcaccac tgatcacgcc agcaccgatt
     4621 cgaccgcagg cgcgggaagg catgcctttc ccgcgccttt ttgcatcggc gccccccata
     4681 ttgctccgcc tttcgccttt gtgacaatct gcgacattgc tggcccgccc gttcacttac
     4741 gcgaaaggcc ggtgccgcta cacaggtctt gtcagagcga gacgaggcgg gtttcccccc
     4801 tgttgcccca gcctcaccct ccttgctccg gctgcgtgac gaacgcaaat gctgaacccc
     4861 cgcctcccag cccccggagg cgggggttct tctttgcagc cacccattgc tcttgccgcc
     4921 gctgccgcct acagccgcgc gcatgctgtc tgttgccaca tggaacatca actcggtgcg
     4981 gctgcgcatg ccgctggtgg aacgctttct ggccgaggag gcgcccgatg tgctgtgcct
     5041 ccaggaaatc aagacggtgg ccgagctgtt cccggccgag gccttcgcgg cgctgggcta
     5101 cacctatcag gcggtgcatg ggcagaaggg ctatcatggc gtggcaacgg tggccagggt
     5161 gccgctgcgc gaagtgtcgc gccgcgactg gcaggccaat ggcgaggcgc gccatgtggg
     5221 cgtggaactg cccgatcacg gcatcactat cgagaatgtc tatatcccgg ccggcggcga
     5281 tattcc
//
LOCUS       DASRYE010000264         9768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_210833,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000264 DASRYE010000000
VERSION     DASRYE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210833"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFF98_13635"
     CDS             <1..372
                     /locus_tag="VFF98_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZU64719.1"
                     /translation="SLIPNRYSGAPGIRWQNVFIMAGVPSITAGMLDALTGTLEGGLP
                     VLSGTVGCWVGESEVASLLGETEKAHEGVAIGSYPFFREGRTGANFVVRTVDAGQLDA
                     CLAAISAGLVAMGREPVAGGI"
     gene            378..941
                     /locus_tag="VFF98_13640"
     CDS             378..941
                     /locus_tag="VFF98_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64720.1"
                     /translation="MKAAMLALGALMAHPAQAAPATPAELAAALARHDSATLALEEWC
                     AARGIATPARVLAEPVFGGTPEEPIDLRDLLGVPAGAKLGYRHVRLACGGVVLSDAQN
                     WYVPARLTLDMADTLANTDAPFGKVVAPLHFTRTRLASAENRLPGCPDDTVLAQRALL
                     RLPDGSALALVVECYTAAALGGAGKGK"
     gene            1013..1573
                     /locus_tag="VFF98_13645"
     CDS             1013..1573
                     /locus_tag="VFF98_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZU64721.1"
                     /translation="MTEASYLSGRLLLALPGMPDPRFEEAVIALCVHDAQGALGVGVG
                     ALLDGITLHNLLEDLEIDTGIAPDVDVHYGGPVEPHRGFVLHSPEWQGDGTLVVGPHW
                     ALSTSRDVLVAIAEGRGPRQWLVALGYAGWGAGQLDGEMRRHGWYAAEGRAGVLFEAE
                     TEGRWAATWRAEGIDPAHLTSVTGSA"
     gene            complement(1581..2669)
                     /locus_tag="VFF98_13650"
     CDS             complement(1581..2669)
                     /locus_tag="VFF98_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZU64722.1"
                     /translation="MAFLPRWPDMAPARLLDDGECVASVLLAIALAHLLGGTNVSWAA
                     FSGFVVMRGHMAETLRRGVLRMVGTLLGAGLALVVTPQVLGRPLLAMAVVVLVATISL
                     YGAITARRAYAWLLFGLTFVMIVIDKLERPQVVLAHFAESRVMETLAGTLACVVVGLA
                     SALTLRRRWPAAAPATPEGAGWQPLALRHAAQGGAALAVMLSLGQWLPLPAQVQAAIT
                     IMAVMLVPVTAVGPGKVAMVSRRIWQRIVGCVAGAAVAGVALLAAQGQAPVLIAATAL
                     GVAVGRHLENGAQSRRYVGTQFTLAILVTLVPDSYAHAELAPGLERLWGVLVGMAILE
                     PVLICWHLLRPLKAPAPAPAGQGGTETE"
     gene            complement(2782..3483)
                     /gene="rplA"
                     /locus_tag="VFF98_13655"
     CDS             complement(2782..3483)
                     /gene="rplA"
                     /locus_tag="VFF98_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZU64723.1"
                     /translation="MAKQTKKQQALGVTLGDNQKLYAVDEAIQFLKDLKSAKFDETLE
                     VSLNLGVDPRHADQMVRGMVTLPSGTGKDVKVAVFARGDKAEAALAAGADRVGAEDLL
                     EDMQAGNLDYGRVIATPDMMGIVGRLGKLLGPKGLMPNPKLGTVTPNVAEAVKAAKGG
                     QIEFRVEKAGIIHGGIGKLSFSNEALRANFDAFVDAIVKAKPSGAKGKYVRKVGLSSS
                     MGPGLKIDVAQVHGS"
     gene            complement(3488..3919)
                     /gene="rplK"
                     /locus_tag="VFF98_13660"
     CDS             complement(3488..3919)
                     /gene="rplK"
                     /locus_tag="VFF98_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZU64724.1"
                     /translation="MAKKIDGYIKLQIAAGQAKPSPPIGPALGQRGVNIMGFCKEFNA
                     ATQEVEAGTPLPTIITVYSDKSFSFVTKTPPATFFIKKALGLKSGSKTPGKASAGTIT
                     RAQLADIATLKMKDLNANDLDQAVKIIEGSARSMGIQVVEG"
     gene            complement(4112..4648)
                     /gene="nusG"
                     /locus_tag="VFF98_13665"
     CDS             complement(4112..4648)
                     /gene="nusG"
                     /locus_tag="VFF98_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZU64725.1"
                     /translation="MARWYIIHAYSGFENKVKEAIIAEAERLGLSALVEQVEVPSEHV
                     TEVKRGKKVQVERKTMPGYVLAKLAMNDDIYHLVKNTAKVTGFLGLNGKPQAISEKDA
                     ARYFGAREAVAAQVSKHVAVDYEIGDSVKVLDGPFASFNGVVEELDFEKNRVKVSVSI
                     FGRATPVELDFDQVELSK"
     gene            complement(4677..4889)
                     /gene="secE"
                     /locus_tag="VFF98_13670"
     CDS             complement(4677..4889)
                     /gene="secE"
                     /locus_tag="VFF98_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZU64726.1"
                     /translation="MDERSAKPRVSPAEFFKQVKVEAGKVVWPTRQETTSTAIFVAIM
                     MSVLALFFLGVDTLFGKLVQFLLSLA"
     gene            complement(4996..6498)
                     /locus_tag="VFF98_13675"
     CDS             complement(4996..6498)
                     /locus_tag="VFF98_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64727.1"
                     /translation="MSVATLPRTAEADQTGVRMVSGAGAQSRVWLWLAVVLSLVSALP
                     VMVAQYPQLTDYPAHLARYHVMLDNGHSPWLAKYYAFLWTWTGNLGVDLLIRPFAAVF
                     GLELGGRIIVGLIGPLSGLGIVAVEWALRRRVSFGSFLALSLIWSPMLVMGLINYALG
                     QAVAFWAFAAWVWLEGKRWRPWVFLPLSLVVWLCHLSAWGVLGIMVFGYEWHRQKSWR
                     ALIAPWPLLAPLAVMLLVHGSTSGEFSYGHHWWYYKFAMWLRATRDTNETLDSWTLVL
                     VGVAIVVATALRRIDGRLGWGALILVVGSWGIPRHISGGDFADYRMITAGLAVACMAI
                     DWPQRWPVLSLAAVGLFLLRISVTSQGWYANSLEMAEQLKALDYVPEGASVASAVLTP
                     VEFWPINPFEHIGAYSVVRKDALCNANFAVPHIHMLHLHKTGYVDPSQRLFQPYRAKV
                     DMAKFKPAEDADYLWYVGPRAPDTMPAGAVIIWRGPHSFLARLAKTAEKD"
     gene            complement(6746..8005)
                     /locus_tag="VFF98_13680"
     CDS             complement(6746..8005)
                     /locus_tag="VFF98_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZU64728.1"
                     /translation="MQQEIQGEIAGGPSAPVNGGTADVLIFAIEAGRYGPARLPQNLD
                     AVGLKVATLCPEDNVLAQTDHAACHYRLPSSRSAPQLARALMAALTRSQARLVIPADE
                     QTVVLLHHFIRGGCAKIIGVEGLAVLVRSLGRPDRLGAMLFKSQTLALARDLGLRVPP
                     GGTAGFAGEALHIADQLGYPVYVKRSFGWSGLGVERCADAAALVAAMGTRPGGSLVAG
                     AKRQLRHWLQRDWYPETSDMDVQGMVAGKPAFYCALAWQGQMVGGYAGEALSTTSDTG
                     PSSEVRVGAHAGMGEATRRMIEALGCNGWVGFDFILEEGTGEPVLLECNPRPIQVCHL
                     GYRIGVDLAAELARLLRGGAMPAKPLEASQELDVVLFPYALDPARQRPGALGDIPGKD
                     AKLMKYGASLAGGGAALRRGWWRGPAV"
     gene            8287..9051
                     /locus_tag="VFF98_13685"
     CDS             8287..9051
                     /locus_tag="VFF98_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64729.1"
                     /translation="MRLADSPYSDPLHGSGSFRQQTDLRNRLRAIALTLAIMALFAAG
                     LVGLGVVSADRPGNGHNLVSMSFSADSNSQKAQHKSHQPTPKTQQSTAQTSPTPKPVE
                     PVTPVTPPGFLHLSRDEFASTDISHMAHQSREGGAASSADSQAAYGPGEGPGGARLYV
                     AEWYREPTHAELAPYLPHNDSDGEWAMIACRTVEHYHVEDCQELTESPPGSGLARALR
                     QAAWQFLVRPPRMNGQAMIGSWVKIRFEFKTVKARG"
     gene            9149..>9768
                     /locus_tag="VFF98_13690"
     CDS             9149..>9768
                     /locus_tag="VFF98_13690"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZU64730.1"
                     /translation="MDAFSPSERQHGWVFSTASAVVAGLIYFLVSAAMVRLTTDGQGI
                     ATMWPGNALILALLLLKDRPAWPALLTGCALGNLAANLVTRGHIGGSAGYAAINLLEI
                     WVAARLLHEPVRRRGILRYSASVRQFVLVTGVLAPASSALFGAAVASLFYHQPFLRAA
                     ITWFACDALGLMVFTPFFHNLFSGALAGWWRAQGHARRLEALAVLGL"
BASE COUNT         1650 a         3509 c         3068 g         1541 t
ORIGIN      
        1 agccttatcc ccaaccgcta ttccggcgcg ccgggcatcc gctggcagaa tgtgttcatc
       61 atggcgggcg tcccctccat caccgccggc atgctcgatg cgttgaccgg cacgctggaa
      121 gggggcctgc cggtgctctc cggcacggtg ggctgctggg tgggcgaaag cgaagtggcg
      181 agcctcctcg gcgagacgga aaaggcccat gagggcgtgg ccatcggcag ctatcccttc
      241 ttccgcgagg ggcgcacggg cgccaatttc gttgtccgca cggtggatgc gggccagctg
      301 gatgcctgcc ttgccgccat cagcgccggc cttgtggcca tgggccgcga gccggtggca
      361 ggcgggatct gagcgccgtg aaagccgcga tgctggcgct gggcgccctg atggcccatc
      421 ccgcccaggc ggcccctgcc acacccgccg aactggccgc cgccctcgcc cggcacgaca
      481 gcgctacgct ggcgctggag gaatggtgcg cggcccgcgg cattgccacg ccggcgcgcg
      541 tccttgccga acccgtcttc ggcggcacac ccgaggagcc gatagacctg cgcgacctgc
      601 tgggcgtgcc ggcgggcgca aagctgggct atcgccatgt gcggctggcc tgcggcgggg
      661 tggtcctgtc ggacgcgcag aactggtatg tgcccgcccg cctcacgctc gacatggccg
      721 atacgctggc caacaccgat gcgcctttcg gcaaggtggt ggcgccgctg cacttcaccc
      781 gcacgcgcct tgccagcgcc gagaaccgcc tgcccggctg cccggatgat accgtgctgg
      841 cgcaaagggc gctgctgcgc ctgcccgatg gcagcgcgct ggcgctggtg gtggaatgct
      901 acaccgccgc cgcgctgggg ggcgccggca agggcaagtg agcgcatggg tgaggaaggc
      961 gcggcacaag cgccccttcc ctttcccccg attcgcgcct agtctccatg gcatgaccga
     1021 ggcctcctac ctttccggcc ggttgctgct ggcgctgccc ggcatgcccg atccgcgctt
     1081 cgaggaagcc gtgatcgcgc tgtgcgtgca tgatgcgcag ggcgcgctgg gcgtgggcgt
     1141 gggcgcgctg ctcgatggca tcaccctgca caatctgctg gaggatctgg agatcgacac
     1201 cggcatcgcc cctgatgtcg atgtgcatta tggcggcccg gtggagccgc accgcggctt
     1261 cgtgctccat tcgccggaat ggcagggcga tggcacgctg gtggtcggcc cgcactgggc
     1321 gctcagcacc tcgcgcgatg tgctggtggc gattgccgaa gggcgcgggc cccggcaatg
     1381 gctggtggcg ctgggctatg ccggctgggg cgcgggccag ctggatggcg agatgcgccg
     1441 ccatggctgg tatgccgccg aggggcgcgc aggcgtactg ttcgaggccg aaacggaagg
     1501 ccgctgggcc gccacctggc gcgccgaggg gatcgacccg gcccatctca cctcggtcac
     1561 cggcagcgcc tgaaaggggc ttattcggtc tctgtgccgc cctgccccgc gggtgccggc
     1621 gccggcgcct tcagcgggcg cagcaagtgc cagcagatca gcaccggctc cagaatggcc
     1681 atgcccacca gcacgcccca cagccgctcc agccccggcg ccagctcggc atgggcataa
     1741 ctgtcgggca ccagcgtgac gaggatggcc agggtgaact gcgtgccgac atagcggcgc
     1801 gattgcgccc cgttttccag atgccgcccc accgccacgc ccagcgccgt cgccgcgatc
     1861 agcaccgggg cctgcccttg cgcggccagc agcgcaacgc ccgccaccgc cgcccccgcc
     1921 acgcagccca cgatgcgctg ccagatccgc cggctgacca tcgccacctt gcccgggccc
     1981 accgccgtca ccggcaccag catcaccgcc atgatggtga tcgcggcctg cacctgcgcg
     2041 ggtaagggca gccactgccc cagcgacagc atcaccgcca gcgccgcccc gccctgcgcc
     2101 gcatggcgca gcgccagcgg ctgccagccg gcaccttcgg gcgtggcagg ggccgccgcc
     2161 ggccagcgcc gccgcaaggt gagcgccgag gccagcccca ccaccacaca ggccagcgtg
     2221 ccggccaggg tttccatcac ccgcgattcg gcaaaatggg ccagcaccac ctgcggccgc
     2281 tccagcttgt cgatcacgat catcacgaag gtgaggccga acagcagcca ggcataggcc
     2341 cgccgcgcgg taatcgcgcc atagaggctg atcgtggcga ccagcaccac cactgccatc
     2401 gccagcagcg gccggcccag cacctgcggc gtgaccacca gcgccagccc cgcccccagc
     2461 agcgtgccca ccatgcgcaa aacgccgcgc cgcagcgttt cggccatatg cccgcgcatg
     2521 acgacaaagc ccgaaaacgc cgcccacgac acattggtgc cgcccagcag atgcgccagc
     2581 gcaatcgcca gcagcacaga ggccacgcat tcgccatcat caagcaggcg tgcaggcgcc
     2641 atatccggcc agcggggaag gaaagccatg ccccctctta gcggataggc cgccgctgcc
     2701 aaggccgatg gcgcatgcaa aaaaggccgg gaaggtttcc cctcccggcc ctttttcgtg
     2761 tttttcagcg cgtgccgaaa atcagctgcc gtgcacctgc gcgacatcga tcttcaggcc
     2821 cgggcccatc gacgaggaca gacccacctt gcggacatac ttgcccttgg cgcccgaggg
     2881 cttggccttg acgatggcat cgacgaaagc gtcgaagttg gcgcgcagcg cttcgttcga
     2941 gaacgagagc ttgccgatgc cgccatggat gatgcccgcc ttttccacgc ggaactcgat
     3001 ctggccgccc ttggccgcct tcacggcttc cgccacgttg ggggtcacgg tgcccagctt
     3061 ggggttcggc atcaggccct tggggcccag cagcttgccg aggcgaccca cgatgcccat
     3121 catgtcgggc gtggcgatca cgcggccgta atcaaggttg ccggcctgca tgtcttccag
     3181 caggtcctcg gcgcccacgc ggtcagcgcc agcggccaga gcggcctcgg ccttgtcgcc
     3241 acgggcgaac acggcgacct tcacgtcctt gccggtgccc gagggcagcg tcaccatgcc
     3301 gcgcaccatc tggtcggcgt ggcgcggatc aacgcccagg ttcagcgaca cttccagcgt
     3361 ctcgtcgaac ttggcgctct tcagatcctt caggaactgg atcgcctcgt ccacggcata
     3421 gagcttctgg ttgtcgccca gggtaacgcc cagcgcctgc tgcttcttgg tctgcttggc
     3481 catctgctca gccctccaca acctgaatgc ccatcgaacg ggcggaacct tcgatgatct
     3541 tcaccgcctg atcgagatca ttggcgttga gatccttcat cttcagcgtg gcgatgtcgg
     3601 cgagctgggc acgggtgatc gtgccggccg aggccttgcc cggggtcttc gaacccgact
     3661 tcaggcccag cgccttcttg atgaagaagg tggcaggagg ggtcttggtg acgaacgaga
     3721 acgacttgtc cgaatagacc gtgatgatcg tgggcagcgg cgtaccggcc tccacttcct
     3781 gggtggcggc gttgaattcc ttgcagaaac ccatgatgtt cacgccgcgc tgacccagcg
     3841 cagggccgat cggcgggctg ggcttggcct ggccggctgc gatctgcagc ttgatgtaac
     3901 cgtcaatttt cttggccatg atggcctcct ttcgtcctgt cggggcggtt gcccctcaga
     3961 tcaagcggtg tgacggggcc caaaggcccc ttccgcgcgg atttccccga ttctctcgga
     4021 agaagggtgg gccaatagcg cgattggccc aaaaagcaag gggcaggccc aaagcccgcc
     4081 cccaaacccc ggccgcaaag cgcccggcgg cttacttcga cagctcgacc tggtcgaaat
     4141 ccagttccac cggcgttgcg cgaccgaaga tcgacaccga caccttgacg cggttcttct
     4201 cgaaatcgag ctcttcgacc acgccgttga agctggcgaa ggggccatcg agcaccttca
     4261 ccgaatcgcc gatctcgtag tccaccgcga catgcttgct gacctgcgcg gccaccgcct
     4321 cgcgggcgcc gaaatagcgc gcggcatcct tctcggagat cgcctgcggc ttgccattga
     4381 ggcccaggaa gccggtcacc ttggcggtgt tcttcaccag gtggtagatg tcgtcgttca
     4441 tcgccagctt ggccagcacg tagccgggca tggtcttcct ttcgacctgc accttcttgc
     4501 cgcgcttgac ttccgtcaca tgctcgctcg gcacttccac ctgctcgacc agcgccgaca
     4561 ggcccagccg ctcggcctcg gcgatgatcg cctctttcac cttgttctca aagccggaat
     4621 aggcgtgaat aatataccag cgggccatct ttgcctcttg ttcgtttgcg ggcggctcag
     4681 gcgagcgaca gcaggaactg cacgagcttg ccgaaaagcg tgtcgacacc caggaaaaac
     4741 agcgccagca cgctcatcat gatggcaacg aagatcgccg tggacgtggt ttcctggcgg
     4801 gtgggccaca ccaccttgcc ggcttccacc ttcacctgct tgaagaattc ggccgggctc
     4861 acgcgcggct tggctgaacg ctcgtccatt tccctaatcc ttgcctgtgc cgccacatag
     4921 atcagtgccg ccccggggcc gatgggctgg ccgggatggc gacggtctgc tccaatgtgg
     4981 ggagatgtgg gcgatctagt ccttctctgc ggtttttgca agccgcgcga ggaaggaatg
     5041 cgggccgcgc cagatgatca ccgcgccggc gggcatggta tcgggcgcgc gggggcccac
     5101 ataccacagg tagtcggcat cctcggccgg cttgaacttg gccatatcca ccttggcgcg
     5161 atagggctgg aacaggcgct gcgagggatc gacataaccg gttttatgca ggtgcagcat
     5221 gtggatgtgg ggcaccgcga aattggcgtt gcacagcgca tccttgcgca ccacgctata
     5281 ggcgccgata tgctcgaacg ggttgatcgg ccagaattcc accggtgtca gcaccgcgct
     5341 ggcaacgctg gcgccctcgg gcacataatc cagcgccttc agctgctcgg ccatctccag
     5401 gctgttggca taccagccct ggctcgtcac gctgatgcgc agcaggaaca gccccaccgc
     5461 cgccagcgac agcaccggcc agcgctgcgg ccagtcgatc gccatgcagg ccacggcaag
     5521 gccggcggtg atcatccggt aatcggcaaa atcgccgccg gaaatgtggc gcggaatgcc
     5581 ccagctgccc accaccagga tgagggcacc ccagcccagc cgcccgtcga tccggcgcag
     5641 cgcggtcgcc accacaatgg ccacccccac cagcaccagc gtccagctgt cgagcgtttc
     5701 attggtatcg cgcgtggcgc gcagccacat ggcgaatttg tagtaccacc agtggtggcc
     5761 ataggagaat tcgccgctgg tgctgccatg caccagcagc atcaccgcca gcggcgccag
     5821 cagcggccag ggcgcgatca gcgcgcgcca gctcttctgg cggtgccatt catagccgaa
     5881 caccatgatg cccagcaccc cccaggccga caggtggcac agccacacca ccagcgagag
     5941 cggcaggaac acccacggcc gccagcgctt gccttccagc cacacccagg cggcaaaggc
     6001 ccagaacgcc accgcctggc ccagcgcata gttgatcagc cccatcacca gcatcggcga
     6061 ccagatcagc gacagcgcca agaaactgcc aaagctgacc cgccggcgca gcgcccattc
     6121 caccgcgaca atgccaaggc ccgacagcgg cccgatcagt ccgacgatga tccgcccgcc
     6181 cagttccagc ccgaataccg cggcaaacgg gcggatcagc agatccacgc ccagattgcc
     6241 cgtccatgtc cagaggaagg cgtaatattt ggcgagccac gggctgtggc cattgtcgag
     6301 cattacgtga tagcgcgcca gatgggcggg ataatcggtg agctggggat attgcgccac
     6361 catcaccggc agcgccgaga ccagcgacag caccaccgcc agccacaacc acacccggct
     6421 ctgcgccccc gcgccgctga ccatgcggac accggtctgg tccgcttcag cggtacgggg
     6481 cagggtggca acggacacgc gcgatcctcc tagaacgatg ggcaagcgcc tgtagccctc
     6541 gcccgccgat tggcaagggc gttcttgccc cctcacatct gcgtcagcga taaaggcttc
     6601 gccggtttcg gccaagcccc gcaaatggaa aagcctgcgt atcgcaaaag aacgagaaaa
     6661 agcggcggct tgggccctgg cgttattatc ctgccttttt gatcaggccg cccgctaaaa
     6721 ggggtgcgcc cccttttttt tctgatcaaa ccgccgggcc gcgccaccag ccccggcgca
     6781 gcgcggcgcc gccgccggcc aggctggcgc catatttcat cagcttggca tccttgccgg
     6841 gaatatcgcc cagcgcgccg gggcgctggc gcgccggatc cagcgcatag ggaaacagca
     6901 cgacatccag ctcctggctg gcttccagcg gctttgccgg catggcgccg ccgcgcagca
     6961 gccgcgccag ttccgctgcc agatcaacgc cgatgcgata gcccagatgg cacacctgaa
     7021 tgggccgggg gttgcattcc agcagcaccg gctcgcccgt gccttcctcc aggatgaaat
     7081 cgaagcccac ccagccattg cagcccagcg cctcgatcat ccggcgggtg gcctcgccca
     7141 tgccggcatg ggcgcccacc cgcacttccg agctgggccc ggtgtccgac gtggtggaca
     7201 gggcctcgcc ggcatagccg cccaccatct gcccctgcca ggccagcgcg caatagaagg
     7261 ccggcttgcc cgccaccatg ccctgcacgt ccatgtcgct ggtttcggga taccagtcgc
     7321 gctgcagcca gtggcgcagc tggcgcttgg cgccggccac cagcgagccg cccgggcgcg
     7381 tgcccatggc cgccaccagc gccgctgcat ccgcgcagcg ctccaccccc agccccgacc
     7441 agccgaacga gcgcttcaca tagaccggat agcccagctg atcggcgata tgcagcgcct
     7501 cgccggcaaa gcctgcggtg ccgcccggcg gcacccgcag gcccagatca cgcgccagcg
     7561 ccagcgtctg cgacttgaac agcatcgccc ccagccgatc gggccgcccc agcgagcgca
     7621 ccagcaccgc aaggccctcc acgccgatga ttttggcgca gccgccgcga atgaaatgat
     7681 ggagcagcac caccgtctgc tcgtcggccg ggatcaccag ccgggcctgg ctgcgcgtca
     7741 acgccgccat cagcgcgcgc gccagctgcg gcgcggaacg gctggaaggc aggcgataat
     7801 ggcaggcggc atggtctgtc tgggccagaa cattgtcttc cgggcacagc gtggccacct
     7861 tcaggcccac cgcatcgagg ttctgcggca ggcgcgccgg gccatagcgg ccggcctcga
     7921 tggcaaagat cagcacatcg gccgtgcccc cgttcaccgg cgcgctgggc ccgcccgcga
     7981 tttccccctg aatctcctgc tgcatcgcct tgcctcttcg tcgcatcaca cccggcccgc
     8041 aacgcaggcc cccacctccc caacgcaaca gccgtgccaa atgcagcgcg ctgaaaagcg
     8101 gcggttttgc aggcccctgc cagccgccgc ccacgccctt ttgcagaacg ccacgccgcc
     8161 gccgccatcc tttgccaatt gcgacaccca cgcttgcgaa acgcgatgcc cccctgcccg
     8221 gcctgataca ggttgcgaca gtccccccat tgaggcgcag cttaccggcg tgtaggcact
     8281 cagcccatgc gccttgccga cagcccctat tctgaccccc tccatggcag cggttcgttc
     8341 cgccagcaga ccgatctgcg caaccgcctg cgcgccatcg ccctcaccct ggcgatcatg
     8401 gcgctgtttg ccgccgggct ggtgggcctg ggcgtggtct cggcagaccg gcccggcaat
     8461 ggccacaacc ttgtctcgat gagcttcagc gccgacagca acagccagaa agcccagcac
     8521 aaaagccacc agcccacgcc caagacccag caatccaccg cgcagaccag ccccacgccc
     8581 aagccggtcg aaccggtaac gccggtcacc ccgcccgggt tcctccacct gtcgcgcgat
     8641 gagttcgcct cgaccgacat ttcccacatg gcccatcaaa gccgcgaggg cggcgcggcc
     8701 agttcggccg acagccaggc cgcctatggc ccgggcgaag ggcccggcgg cgcgcggctc
     8761 tacgtggccg aatggtatcg cgagcccacc catgccgagc tggcccccta tctgccccac
     8821 aacgacagcg acggcgaatg ggcgatgatc gcctgccgca cggtggagca ttatcatgtg
     8881 gaagactgcc aggagctgac cgaatcgccc cccggatcgg gcctggcccg cgccctgcgg
     8941 caagccgcct ggcagttcct ggtgcgcccg ccgcggatga acggccaggc gatgatcggc
     9001 agctgggtaa agatccgttt cgaattcaaa accgtcaagg cgcgcggctg acaggacata
     9061 aaagtggcaa aaataagcac atacgcttat caccctctca ggcaaattta agccatcctg
     9121 ctccatgcag caaagcatgg gggcgaccat ggatgcattt tctccatcgg aacggcaaca
     9181 cgggtgggtc ttttcgacag ccagcgctgt cgttgccggg ctgatctatt tcctcgtctc
     9241 ggccgcgatg gtgcgcctga cgacagatgg ccagggcatc gccaccatgt ggccgggcaa
     9301 tgccctgatc ctggcgctgc tgctgctgaa agacaggccc gcctggccgg ccctgctcac
     9361 cggctgcgcg ctgggcaatc tggccgccaa tctcgtcacc cgcggccata tcggcggctc
     9421 ggccggttat gcggcgatca acctgctgga aatctgggtg gccgcgcggc tgctgcacga
     9481 gccggtgcgc cggcgcggca tcctgcgcta cagtgcttct gtgcggcaat tcgtgctggt
     9541 gacaggggtg ctggcgccgg ccagcagcgc gctgtttggc gcagcggtgg ccagcctgtt
     9601 ctatcaccag ccgttcctgc gcgcggcgat cacctggttt gcctgcgatg cgctggggct
     9661 gatggtgttc acgccgttct tccacaatct gttttccggc gcgctggccg gctggtggcg
     9721 ggcgcagggc catgcccgcc ggctggaagc gctggcggtg ctgggcct
//
LOCUS       DASRYE010000265        13694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_168195,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000265 DASRYE010000000
VERSION     DASRYE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168195"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFF98_13695"
     CDS             complement(<1..1203)
                     /locus_tag="VFF98_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64731.1"
                     /translation="MATSLDGLPRHHVATHSEKLVITASSLGTVFEWYDFYLYGLLTA
                     IIAAQFLTGLNSTTSFIMALLVFAAGFIVRPFGALVFGHIGDLVGRRYTFIITLAVMG
                     LSTFLVGCLPTYKQVGVAAPIMLVGLRMFQGLALGGEYGGAATYVAEHAPNSRRGLYT
                     SWIQITATAGLAMALLIVISVRSPVTGVGEEAFKEWGWRIPYLLSGLFLVVGLWLRLK
                     LHESPVFQKMKDEGTASKAPLSEAFGQWKNVKIVIIAFFGAIMGQAVVWYTGQLYAMF
                     FLEKMLKLDGLTANMLIITALLLATPFFLIFGTLSDRIGRKPVILTGCLLASVSLFPV
                     FHALTEAANPALAHAQANAPVTVTANPGECSSQFDPVGANKFDTTSCDIVKNVLAKAG
                     VNYRNVEAP"
     gene            complement(1240..2769)
                     /locus_tag="VFF98_13700"
     CDS             complement(1240..2769)
                     /locus_tag="VFF98_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HZU64732.1"
                     /translation="MMLKGKKAVFGLLMGASALVSPQVVWAKGSAAHKHVAAKPTARE
                     AALLARIEKLEAEMADMRAHKDAPSPAADAELAEAKASAAAARQAAAVAEAAASTAAD
                     KSSDAVTRVAAIEKARPQGFRSGNSTVNVSGYVKLLMTSARYSGGDDSTNTLGRDIYL
                     PGSIPVYNPASPTSPTRVTDFSAKQSRLWVDASTKLGSHTLKAYIEADFQAAPGTPMA
                     AGQGTQRTTNAYDFAMRRAFIQLDRWTFGQDFTNFSPVSVLPESTDYVGGVDGLIFVR
                     QPQLRYALPVQKGLNLIMSVENPETASSTVGSTTMNENGEDHAPDTTARFEYNGNLGM
                     LSLAGMVRQLRVDNAKVAAAHVVDDRLAWGASAAGKVFLNAKHTSDFRFEATYGDGLG
                     RYLGLNFSPDAVLMANGKLGKVSQLGVFGAGHIALNDKWRVNLIGSYQQVYYAANIDR
                     SSAGIASYNKQAWSAAGNLFYSPFHNVDLGVEYRHGNRELVNGLHGAVDRMEFAAKYG
                     F"
     gene            2973..4937
                     /gene="acs"
                     /locus_tag="VFF98_13705"
     CDS             2973..4937
                     /gene="acs"
                     /locus_tag="VFF98_13705"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZU64733.1"
                     /translation="MGETIYPVPAEWAETALIDDARYQQMYARSVEDPEGFWGEEARR
                     IDWIRPFSQVKNTSFHKDDFGIRWFADGTLNLAANALDRHLESRGEQIAILWEPDHPD
                     TPDRRITYRQLHADVCRLANVLKARGVQKGDRVTIYLPMVPEAAVAMLACARIGAIHS
                     IVFAGFSPEALAGRVEDCASRIILTADEGLRGGKKVPLKKNVDEALAILDASAAPRVD
                     TVLVLAHTGGAVPTDPARDVDWHSATAAQSTDCPPEEMNAEDPLFILYTSGSTGKPKG
                     VLHTTGGYTVWASMTHQYVFDYRPGQIYWCAADIGWVTGHSYVVYGPLVNGGTTVMFE
                     GVPNFPDPSRFWQVVDKFGVEIFYGAPTALRALMREGDEWVAKTSRKSLRLLGSVGEP
                     INPEAWEWYHRVIGEHRCPIVDTWWQTETGGVMITPLPGATALKPGSATRPMFGVKPL
                     LLDNDGTVMDGPGDGCLVIADSWPGQMRGVWGDEERFFSTYFTTFAGYYFTGDGCRRD
                     EDGYHWITGRIDDVINVSGHRMGTAEIESALVAHHDVAEAAVVGMPHEIKGQGIYAYV
                     TTNAGVEPDEVLRKALIAWVRHEIGPIATPDVIQFAPGLPKTRSGKIMRRILRKIAEN
                     DVSNLGDTSTLADPSVVDHLLANRPQLASA"
     gene            5005..5685
                     /locus_tag="VFF98_13710"
     CDS             5005..5685
                     /locus_tag="VFF98_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU64734.1"
                     /translation="MGDQFHPSLTAAAGAILIADDHPLFRQALALAIPRVRPGARIVE
                     AGTLAAAARAASETPGLSLILLDLKMPGAVGYSGIALLHAEQPDVPILVVSSAEGASV
                     AQEARSFGARGFLSKDSDLGTIEAAIQRVLGHSAACATPAPAPGAAAENDLDTIRGEV
                     ARLTPTQLKVLLAVLDGQLNKQIAHNLGITEATVKAHMTAIMRKLDVKNRTQAALVAR
                     SLGLDLAH"
     gene            complement(5682..9059)
                     /locus_tag="VFF98_13715"
     CDS             complement(5682..9059)
                     /locus_tag="VFF98_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HZU64735.1"
                     /translation="MSVHAAALFALALMGLLFGVAALADKRLPPSARRGRMRHGAYTL
                     SLGVYCSSWTIYGAVGSAVREGWSYLPIYLAPICVLVCAPGFIRRLANAVAEEQATTV
                     SDFLAARFGHDAVVARLVTAICLAGSVPYMALQLRSLGMAMSIVTGQGITTPTMVVAA
                     ILLVAFAILFGARRFEVASRSEGMVYAMGVESLIKIASLAVVAGVAVLALVAAPAAST
                     AHGLGELAERFRPVHLSLDTGVIALISAMAVLALPRQFYMAQVEARDARDLPAARLGL
                     AAYLTFMALMVLPIALAGASLLDARVAPDLWVLQLPLAAGYRPIVAVALMGGISAAAA
                     MVITDATALATMVSNDLVFPTLLRSGGRQAEAGALGRRMLLVRRVSICAIVALALAWA
                     LLVAPGQSLASIGLVAFAAMAQFTPHLLLATGRRRHDAMAARASLATGFALWLYTLAL
                     PPILPESWRLALAGGLFDPLRLFGIGHAAPLLHGVAWSLGANIAVFAATTARRMPAPA
                     LPRLVRWHRQVSDLAGLVDLTASFVGRERAEREFPEARRGLQISARAAQRARKLIGGV
                     VGTSSARTLVASALAGGTMSLAEVTRLLDERGQSLGFSRQLLAATFEHIDAGISVVDA
                     SLNLVAWNTRYEQLFDYPPGMLYVGVPVADLIRHNALRGHFGPGDVEPQIARRLNHLR
                     RGMVHVFERRHGDGRVIKTVGGSMPGGGYVTSYTDITEEASVREELERTLASLEQRVE
                     QRTAALTEANRQLQRATADKTRFLAAASHDLLQPLHAARLFTAALARDVPESASALVG
                     RVDSAILAAEDLLRALLDISKLDAGGVRPRPEALALAPFLTGLVEGLRPMADAKGLRL
                     RLGALVGEVCTDPGLLRSLLQNFIVNALRYTPAGGVLVGARRRAGPEGQAGLRIDVVD
                     SGIGIPPDQIEAIFGEFTRLGAVEAEGLGLGLALSERIARLLGARIEVASNPGRGSRF
                     SLWLPLAPAAEEVPARLPAATGAAAGTARALCVLVLDNDPLTVEATSALLASLGHRPQ
                     GAGDGAAALALAEAHRLDAVLADYRLDATDAGGENGLGVVAALRRRHPGLPAVLITAE
                     SGGDVAEAARLAGVPVLAKPVAPERLEAFLAGVCASA"
     gene            complement(9150..10211)
                     /locus_tag="VFF98_13720"
     CDS             complement(9150..10211)
                     /locus_tag="VFF98_13720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZU64736.1"
                     /translation="MNDCSSPPGWRPRLVNLLDDFNLGGVSRALGIFESEVLRAGVRS
                     SIVAVRPDTLLAEHYDADIIVLHFPPNWRRLAYVASLRLRNPQACLIWVEHSYTAAWA
                     RAKVRRRRRFGLLLKLAYRLATRIVSVSEGQAAWLRAGIGRAAGRVEVIHPYTENPGL
                     ALLAPPLRQPGAPLRIGAYGRFCEQKGLDNLIRAHRAGWMAGTELVIGGFGEEEALLR
                     GLAGDAPDIHFYGKVTQVAEFLASCDCVAVPSRWEAYGMVANEAREAARPILVAPVDG
                     LPEQVGAAGLIVDFTDRASVEAACAQLQEADLAAMGLAGRAATRGCAERRAGRWAQLL
                     AGLPLVRRPGHGLAAVAAG"
     gene            complement(10208..11485)
                     /locus_tag="VFF98_13725"
     CDS             complement(10208..11485)
                     /locus_tag="VFF98_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZU64737.1"
                     /translation="MVHRRLGGGYGPLARGLGAFGGAELATRVVRLVATVVIARRLAP
                     DIVGEAALALTIFEIVRVLERTGTGQKIVAVPDDELAATCNTANRLSWLWTMGLMGVQ
                     WLAAAGLALFAHRPVAAQILAVLALVYPFMAGGHVQYHLALREGRTSQLAAISGAQAI
                     ADHILTALLLLIWPDPWSLALPKLLTAPQWLIMTRRARPWLRDAGAGHIPLRGMLRYS
                     ASILAAEAMVALRTQGDNLIIAGTMGTTALGTYYFAFNAGLGIVSSLIGAFRQVAFSM
                     LCAARDAAARVVALRRVLIGGVLAFVPVIALQSMAAAFYVPIIFGHKWAFAAPLIGTL
                     CLAGLPLLASAITASWLRAEGRVGSDAASAALACAAALLGLFVGTRFGTLEAAAVGLV
                     SGQALASAIYAVRVLGPALRNHVSSMRQEEILT"
     gene            complement(11763..12833)
                     /locus_tag="VFF98_13730"
     CDS             complement(11763..12833)
                     /locus_tag="VFF98_13730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZU64738.1"
                     /translation="MRLAIATDAWFPQVNGVVRTLSATVNELTARGIEVELITPERFL
                     TVPMPGYSQIRLAVSPRFSARRMLDRFRPDIVHIATEGPIGWSARGWCRSRRVPFTSA
                     FHTRFPEYLAVRTGIPADYFWPVMRHFHGPSRAVMVATPSLAGELNERGLAHTHRWSR
                     GIDRSLFAPVGALLPEMADLPRPILLNVGRVAPEKNIEAFLDADLPGTKVVVGDGPAL
                     ADMQRRYPGVHFLGALAGERLAAAYRTADCFVFPSRTDTFGLVVIEALSCGVPVAAYP
                     VPGPLDIIGHDGRGHEGEAPGPVGALDEDLSAAITRALAVDREAAASFGARFSWERAT
                     DQFLAAIHAALEAASLPRAAVA"
     gene            complement(12905..>13694)
                     /locus_tag="VFF98_13735"
     CDS             complement(12905..>13694)
                     /locus_tag="VFF98_13735"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZU64739.1"
                     /translation="KRKYRTVWISDIHLGTRGCNAVMLVEFLKSIETDTIYLVGDIID
                     GWRLSRGWYWPDAHNEVIRRILKMAHRGTRVVFVCGNHDEMLRPYAGLTFGGVELVLE
                     CIHETVDGRRLLVTHGDAFDAVVLYHRWLAFLGDKAYSAMMRVNVVFNGIRRRMKLPY
                     WSLSAYLKKRVKNAVQFIGRFEEAVAHAAIERGVDGVVCGHIHSAEIREIEGITYYND
                     GDWVESCTALVEDVAGVISIVDWAQESRVAHALRAPEAAAQTPA"
BASE COUNT         2415 a         4827 c         4485 g         1967 t
ORIGIN      
        1 gggcgcttcc acgttgcgat agttgacacc ggccttggcg agcacgttct tcacgatgtc
       61 gcagctggtg gtgtcgaact tgttggcgcc cacagggtca aactgcgagg agcattcgcc
      121 aggattggcc gtcaccgtta ccggcgcatt ggcctgggca tgggccagcg ccggattggc
      181 cgcttctgtc agcgcgtgga acaccgggaa caggctgacc gaggccagca ggcagccggt
      241 gaggataacc ggcttgcggc cgatgcgatc ggacagcgtg ccgaagatga ggaagaaggg
      301 cgttgccagc agcagcgcgg tgatgatcag catgttggcc gtcaacccgt cgagcttcag
      361 catcttctcc aggaagaaca tcgcatagag ctggccggta taccacacca ccgcctggcc
      421 catgatggcg ccgaagaagg cgatgatgac gatcttcaca ttcttccact ggccaaaggc
      481 ttccgacagc ggcgccttgg aagcggtgcc ctcgtccttc atcttctgga acaccgggct
      541 ttcatgcagc ttcagccgca gccacagccc caccaccagg aacaggcccg acagcagata
      601 gggaatgcgc cagccccatt ccttgaaggc ctcctcgccc acgcccgtca ccggcgagcg
      661 cacggagatg acgatcagca gcgccatcgc cagccccgcc gtggcggtga tctggatcca
      721 gctggtgtag aggccgcgcc tggaattggg ggcgtgctcg gccacatagg tggcagcgcc
      781 gccatattcg ccgcccagcg ccaggccctg gaacatgcgc aggcccacca gcatgatcgg
      841 cgctgccaca cccacctgct tgtaggtggg caggcaaccg acgaggaagg tggacaggcc
      901 catcaccgcc agggtgatga tgaacgtata ccgccggccg accagatcgc cgatatggcc
      961 gaacaccagc gcgccaaacg gccgcacgat gaagccggcg gcaaacacca gcagcgccat
     1021 gatgaacgag gtggtggaat tgaggccggt gagaaactgc gcggcgatga tcgccgtcag
     1081 caggccatag agatagaaat cataccattc gaacaccgtg cccaaggagc tggcggtgat
     1141 caccagcttc tcgctatgcg tggccacgtg gtgtcgtggc agcccgtcca aagatgttgc
     1201 catgaaccct ctccatctgt tttatctatt tgttttggct tagaaaccgt atttcgcggc
     1261 aaattccatg cggtccaccg cgccatgcag gccgttcacc agctcgcggt tgccgtggcg
     1321 gtattccacg cccagatcga cattgtggaa gggcgaatag aagaggttgc cggccgcgct
     1381 ccacgcctgc ttgttgtagc tggcgatgcc ggcgctgctg cgatcgatgt tggcggcata
     1441 atagacctgc tggtagctgc cgatcaggtt cacgcgccat ttgtcattca gcgcgatatg
     1501 cccggcgcca aacacgccca gctggctgac cttgcccagc ttgccattgg ccatcagcac
     1561 cgcatccggg ctgaagttca ggcccagata gcggcccagc ccatcgccat aggtcgcctc
     1621 gaagcggaaa tcggaggtgt gcttggcatt gaggaagacc ttgcccgcag cgctggcgcc
     1681 ccaggccagg cgatcgtcca ccacatgggc ggcggccacc ttggcgttgt ccacccgcag
     1741 ctggcgcacc atgccggcca ggctcagcat gcccagattg ccgttatatt cgaagcgcgc
     1801 cgtggtatcg ggcgcgtggt cctcgccatt ctcgttcatc gtggtgctgc caacggtgct
     1861 cgaggcggtt tccgggttct ccaccgacat gatgaggttc agccccttct gcaccggcag
     1921 cgcatagcgc agctgcggct ggcgcacgaa gatcaggcca tccacgccgc ccacgtaatc
     1981 ggtgctttcc ggcagcacgg aaaccggcga gaagttggtg aaatcctggc cgaaggtcca
     2041 gcggtcaagc tggatgaagg cgcgccgcat ggcgaagtcg taggcgttgg tagtgcgctg
     2101 ggtgccctgg ccggcggcca tcggcgtgcc gggggcggcc tggaagtcgg cctcgatata
     2161 ggccttcagc gtgtggctgc ccagcttggt gctggcatcc acccacaggc ggctctgctt
     2221 ggccgagaaa tcggtgacgc gggtgggcga ggtggggctg gccggattgt agaccggaat
     2281 cgagccgggc agatagatgt cgcggcccag cgtgttggtg gaatcatcgc cgccgctgta
     2341 gcgcgcgctg gtcatcagca gcttcacata gcccgagacg ttgaccgtgc tgttgcccga
     2401 acggaagccc tgcggcctgg ccttctcgat ggcggccacg cgggtcacgg catcgctgct
     2461 cttgtcggca gcggtgctgg cggcggcctc ggccacggcg gcggcctggc gggcagcggc
     2521 ggcagaagcc ttcgcctcgg ccagctcggc atcggcggcg ggcgatggcg catccttgtg
     2581 ggcgcgcatg tcggccatct cggcttccag cttctcgatg cgggccagca gcgctgcctc
     2641 gcgcgcagtg ggtttggcgg ccacatgctt gtgcgctgcg ctgcccttgg cccacaccac
     2701 ctgcggcgac accagagcac tcgcccccat cagcaggcca aaaacggcct tcttgccctt
     2761 aagcatcatt tcctctccta tcacccgtgt gcttttgtag cgctcgcggt gtgagcgaag
     2821 attgctcgca ggcagcagtc gttctatccc taccatggtc gcccacttac gaccttggta
     2881 tggggtgaat tgggaacatc ggtggataac atgggttgaa acgaggcagg ccccaaggcc
     2941 cgcccgcaac ccttagagag gagtgcttgg cgatggggga aaccatctat cccgtgccgg
     3001 ctgaatgggc cgaaacagcc ttgatcgacg atgcgcgcta tcagcagatg tatgcgcgct
     3061 cggtggagga tcccgaaggc ttctgggggg aggaagcgcg ccggatcgac tggatcaggc
     3121 cgttcagcca ggtcaagaac accagcttcc acaaggatga cttcggcatc cggtggtttg
     3181 ccgatggcac gctgaacctg gccgccaacg cgctcgatcg ccacctggaa agccggggcg
     3241 agcagatcgc catcctgtgg gagcccgatc accccgatac gcccgaccgg cgcatcacct
     3301 atcgccagtt gcatgccgat gtgtgccgcc ttgccaatgt gctgaaggcg cgcggcgtgc
     3361 aaaagggcga ccgggtgacg atctacctgc cgatggtgcc cgaggcggcc gtggccatgc
     3421 tggcctgtgc gcgcatcggc gccattcact cgatcgtctt tgccggcttc agccccgagg
     3481 cgctggccgg ccgcgtggag gattgcgcca gccgcatcat cctcaccgcc gacgagggcc
     3541 tgcgcggcgg caagaaggtg ccgctgaaga agaatgtcga cgaggcgctg gcgatcctcg
     3601 atgccagcgc tgcgccccgg gtagacaccg tgctggtgct ggcccacacc ggcggcgccg
     3661 tgcccaccga tcctgcccgc gacgtcgact ggcacagcgc caccgccgcc cagagcaccg
     3721 attgcccgcc cgaggaaatg aacgcggaag acccgctgtt catcctctac acctccggct
     3781 ccaccggcaa gcccaagggc gtgctgcaca ccaccggcgg ctacaccgtc tgggcctcga
     3841 tgacgcatca gtatgtcttc gattatcgcc cgggccagat ctactggtgc gcggccgata
     3901 tcggctgggt cacgggccat tcctatgtgg tttacgggcc gctggtgaac ggcggcacca
     3961 cggtgatgtt cgagggcgtg cccaatttcc ccgatcccag ccgcttctgg caggtggtgg
     4021 acaaattcgg cgtggaaatc ttctatggcg cccccacggc cctgcgcgcg ctgatgcgcg
     4081 aaggcgatga atgggtggcc aagaccagcc gcaagagtct gcgcctgctg ggcagcgtgg
     4141 gcgagccgat caatccggaa gcctgggaat ggtatcaccg ggtgatcggc gaacaccgct
     4201 gccccatcgt cgacacctgg tggcagacgg aaaccggcgg cgtgatgatc accccgctgc
     4261 ccggcgccac cgcgctcaag cccggcagcg ccacccggcc gatgttcggc gtgaagccgc
     4321 tgctgctcga caatgacggc acggtgatgg acggtccggg cgatggctgc ctggtcatcg
     4381 ccgacagctg gcccggccag atgcgcggcg tgtggggcga tgaggaacgc ttcttctcca
     4441 cctatttcac cacctttgcc ggctattact tcaccggcga cggctgccgc cgcgacgagg
     4501 atggctatca ctggatcacc ggccgcatcg atgatgtaat caacgtctcg ggccaccgca
     4561 tgggcaccgc cgagatcgag agcgcgctgg tggcccacca tgatgtggcc gaggccgccg
     4621 tggttggcat gccgcacgag atcaagggcc agggcatcta cgcctatgtg acgaccaacg
     4681 ccggggtgga acccgacgag gtgctgcgca aggcgctgat cgcctgggtg cgccacgaga
     4741 tcggcccgat cgccacgccc gatgtcatcc agtttgcccc gggcctgcct aaaacccgct
     4801 cgggcaagat catgcgccgc atcctgcgca agatcgcgga aaacgatgtc tccaacctgg
     4861 gtgacacctc tacccttgcg gatccgtcgg ttgtagacca tctgttggcc aacaggccgc
     4921 agctggcaag cgcctgaggc aaaaccgcat cccctttccg gcgcggcttt gccctttggc
     4981 cgcgccggaa ccatggggga cccattggga gatcaatttc atccatcgct gacggccgct
     5041 gccggggcga tcctgatcgc cgacgatcat ccgctgtttc gccaggcgct ggcgctggcg
     5101 atcccccgcg tgcggcccgg cgcgcgcatc gttgaagccg gcacgctggc cgctgccgcc
     5161 cgcgccgcca gcgaaacgcc cggcctcagc cttatcctgc tcgatctgaa gatgccgggc
     5221 gcggtgggct attcgggcat tgccctgctc catgccgaac agcccgatgt gccgatcctc
     5281 gtcgtctcca gcgcggaagg ggcatcggtg gcgcaggaag cgcgcagctt tggcgcccgc
     5341 ggcttcctct cgaaagacag cgaccttggc acgatagagg ccgccatcca gcgcgtgctg
     5401 ggccacagcg ccgcctgcgc cacccctgcc cctgccccgg gcgccgccgc cgagaatgac
     5461 ctcgacacca tccgcggcga agtggcgcgc ctcacgccca cccagctgaa ggtgctgctg
     5521 gccgtgctcg atggccagct caacaagcag atcgcccata atctgggcat caccgaggcc
     5581 acggtgaagg cgcatatgac cgcgatcatg cgcaagctgg atgtgaagaa ccgcacgcag
     5641 gcagcgctgg tggcccgctc gctggggctg gatctggcgc attaggcgct ggcgcacacc
     5701 ccggccagaa acgcctccag gcgctctggc gccaccggct tggccagcac cggcacgccg
     5761 gccagccgcg ccgcttccgc cacatcgccg ccgctttcgg ccgtgatcag cactgctggc
     5821 aggcccggat ggcgccggcg cagcgcggcc accacgccaa ggccattctc gccgccggca
     5881 tcggtggcat ccagccgata atcggccagc acggcatcca gccggtgcgc ctctgccagt
     5941 gccagcgcgg ccgcgccatc gcctgccccc tgcggccggt ggcccaggct ggccagcagc
     6001 gccgaggtgg cctccaccgt cagcggatca ttatccagca ccagcacgca tagcgcccgc
     6061 gccgtgccgg ccgccgcccc tgtggccgcg ggcaggcggg cgggcacctc ctctgccgcc
     6121 ggggccagcg gcagccacag gctgaagcgg ctgccccgcc cggggttgga ggccacttcg
     6181 atgcgcgcgc ccaggaggcg ggcaatgcgt tccgacaggg ccagccccag ccccagccct
     6241 tcggcctcca ccgcgcccag ccgggtgaat tcgccgaaga tcgcctcgat ctggtcaggc
     6301 gggatgccga tgccgctgtc caccacatcg atcctcaggc ccgcctgccc ttctggcccg
     6361 gcccgccgcc gcgcccccac cagcacgccg ccggccggcg tgtagcgcag cgcgttgacg
     6421 atgaaattct gcaacagcga gcgcagcagc cccggatcgg tgcacacctc gcccaccagc
     6481 gcccccagcc gcagccgcag gcccttggca tcggccatcg gccgcagccc ttccaccagg
     6541 ccggtgagga agggcgccag cgccagtgcc tcgggccggg ggcgcacgcc gccggcatcc
     6601 agcttggaaa tgtcgagcag ggcgcgcagc aaatcctcgg cggccagaat ggcgctgtcc
     6661 acccggccca ccagcgcgct ggcgctttcg ggcacatcgc gcgcaagcgc ggcggtaaac
     6721 aggcgggcgg catgcagcgg ctgcagcaga tcatggctgg cagcggccag aaagcgggtc
     6781 ttgtcggcgg tggcgcgctg cagctggcgg ttggcttccg tcaaagcggc ggtgcgctgc
     6841 tccacccgct gctccaggct ggccagcgtg cgttccagct cctcgcgcac gctggcttcc
     6901 tcggtgatgt cggtatagct cgtcacatag ccgccgccgg gcatcgatcc gcccacggtc
     6961 ttgatcaccc ggccatcgcc atggcgccgc tcgaacacat gcaccatgcc gcggcgcagg
     7021 tggttcaggc ggcgggcgat ctgcggctcc acatcgcccg ggccgaaatg gccgcgcagg
     7081 gcattgtggc ggataagatc ggccaccggc acccccacat acagcatgcc cggcggataa
     7141 tcgaacaatt gctcgtagcg cgtgttccac gccaccagat tgagactggc atcgaccacg
     7201 ctgatgccgg catcgatatg ttcgaaagtg gccgccagca gctggcgcga aaagcccagg
     7261 ctctgcccgc gctcgtccag caggcgggtc acttcggcca ggctcatcgt gccgccggca
     7321 agggcgctgg ccaccagcgt gcgcgccgag gaagtgccca ccacgccgcc gatcagcttg
     7381 cgcgcgcgct gggcggcccg ggcgctgatc tgcaggcccc ggcgcgcctc gggaaattcg
     7441 cgctcggccc gctcgcggcc gacaaagctg gcggtcaggt ccaccagccc ggccagatcc
     7501 gacacctgcc ggtgccagcg caccagccgc ggcagcgccg gggccggcat gcgccgcgcg
     7561 gtggtggcgg caaacacggc aatattggcg ccaaggctcc acgccacgcc atgcagcagg
     7621 ggcgcggcat ggccaatgcc aaacagccgc agcggatcga acagcccgcc cgccagcgcc
     7681 agccgccagc tttccggcag gatcggcggc agcgccagcg tgtagagcca cagggcaaag
     7741 ccggtggcaa ggctggcgcg ggcggccatc gcatcatgcc ggcgccggcc cgtggccagc
     7801 agcagatgcg gcgtgaactg ggccatggcg gcaaaggcca ccagcccgat cgaggccagc
     7861 gactggcccg gcgccaccag cagcgcccag gccagcgcca gcgccacgat cgcgcagatg
     7921 gacacccgcc gcaccagcag catgcgccgc cccagcgcgc ccgcctcggc ctgccgcccg
     7981 cccgaacgca gcagggtggg aaagaccagg tcgttcgaca ccatggtggc cagcgccgtg
     8041 gcatcggtga tgaccatcgc cgctgccgcg ctgatccccc ccatcagcgc caccgccacg
     8101 atcggccgat agcccgccgc cagcggcagc tgcagcaccc agagatccgg cgccacccgc
     8161 gcatccagca ggctggcgcc ggccagcgcg atgggcagca ccatcagcgc catgaaggtg
     8221 agataggccg ccagccccag ccgggcggcg ggcaggtcgc gcgcgtcgcg cgcttccacc
     8281 tgggccatgt agaactggcg cggcagggcc agcaccgcca tcgccgagat cagcgcgatc
     8341 acccctgtgt cgagcgagag atgcaccggg cggaagcgtt cggccagctc gcccaggcca
     8401 tgggcggtgc tggccgccgg cgctgccacc agcgccagca ccgcaacgcc tgccaccacc
     8461 gccagcgagg cgatcttgat cagcgattcg acccccatcg catagaccat gccttccgac
     8521 cggctggcca cctcgaagcg gcgcgcgccg aacaatatgg caaaggccac cagcaggatg
     8581 gcggccacca ccattgtcgg cgtggtgatg ccctggccgg tgacgatcga catcgccatg
     8641 cccaaggagc gcaattgcag cgccatatag ggcacgctgc ccgccaggca gatggctgtg
     8701 accagccgcg ccaccaccgc atcatggcca aagcgcgcgg ccagaaaatc cgaaacggta
     8761 gtggcctgtt cctcggccac ggcattggcc aggcggcgga tgaagccggg cgcgcacacc
     8821 agcacgcaga ttggcgccag atagatgggc agataggacc acccctcgcg cacggcgctg
     8881 cccaccgcgc catagatcgt ccagctggag caatagacgc cgagcgagag cgtgtaggcg
     8941 ccatggcgca tgcgcccgcg ccgggccgaa ggcggcaggc gcttatcggc cagcgccgcc
     9001 acgccaaaca gcaaccccat cagggccagc gcaaaaagcg ccgccgcatg cacactcatc
     9061 ccgttggccc acctgctgcc tgcaacgccc cctgcctaga ccatcggccg ggaaacggca
     9121 aaggccgggg cagcacagga aagcgcagct taaccggcgg ccaccgcggc cagcccatgg
     9181 cccggccggc gcaccagcgg caggcccgcc agcaattgcg cccagcggcc cgcgcgccgc
     9241 tccgcacacc cccgggtggc agcgcgcccg gccagcccca tggcggccag atcggcttcc
     9301 tgcagctggg cgcaggcggc ctccacgctg gccctgtcgg tgaaatcgac gatcagcccg
     9361 gccgcgccca cctgctccgg caagccatcg accggcgcca ccagaatggg ccgcgccgcc
     9421 tcgcgcgcct cattggccac catgccatag gcttcccagc gcgagggcac cgccacgcag
     9481 tcgcagctgg ccagaaattc ggccacttgc gtcaccttgc cgtagaagtg gatgtcgggc
     9541 gcatcgccgg ccaggccgcg cagcagcgct tcctcctcgc caaagccgcc gatcaccagt
     9601 tcggtgcccg ccatccagcc ggcgcgatgg gcgcggatga gattatccag ccccttctgc
     9661 tcgcaaaagc ggccataggc gccaatgcgc agaggcgcgc ccggctggcg cagcggcggc
     9721 gccaacagcg caaggcccgg gttttcggtg taggggtgga tcacttccac gcgcccggcc
     9781 gcccggccga tgcccgcgcg cagccacgcc gcctgcccct cgctcacgct gacaatgcgg
     9841 gtggccaggc gataggccag cttcagcagc aggccaaagc ggcggcggcg gcgcaccttg
     9901 gcccgcgccc aggcggcggt atagctgtgc tccacccaga tcaggcaggc ctgcggattg
     9961 cgcagccgca gcgaggccac ataggccagc cgccgccagt tgggcgggaa atgcagcacg
    10021 atgatgtcgg cgtcataatg ctcggccagc agcgtgtcag gccgcaccgc cacgatcgag
    10081 gaacgcacgc ccgcgcgcag cacctcgctc tcgaaaatgc ccagcgcgcg ggaaaccccg
    10141 cccaggttga aatcatccag caggttgacc agccttggcc gccagccggg aggtgaagaa
    10201 caatcgttca tgtgagaatt tcttcctgtc gcatagaaga aacgtgattg cgcaatgccg
    10261 gccccaatac ccgcaccgca taaatggccg aagccagcgc ctgcccgctc accagcccca
    10321 cggccgctgc ttccagcgtg ccaaagcggg tgccgacaaa caggcccagc agcgccgccg
    10381 cgcaggccag cgccgccgag gccgcatcgc tgcccacccg gccttccgcc ctgagccagc
    10441 tggccgtgat ggccgatgcc agcagcggca gccccgccag gcacagggtg ccgatcagcg
    10501 gcgcggcaaa cgcccatttg tggccgaaga tgatcggcac atagaaggcg gcggccatgc
    10561 tctgcagcgc gatcaccggc acaaaggcca gcacgccgcc gatcagcacc cggcgaagcg
    10621 ccaccacccg cgcggccgca tcgcgcgcgg cacacagcat ggaaaaagcc acctgccgga
    10681 acgcgccgat cagcgaggag acaatcccca gcccggcgtt gaaggcaaaa taataggtgc
    10741 ccagcgccgt ggtgcccatg gtgccggcga tgatgaggtt gtcgccctgg gtgcgcagcg
    10801 ccaccatcgc ctcggcagca aggatggagg cggaatagcg cagcatgccc cgcagcggaa
    10861 tgtggccggc gccggcatcg cgcagccagg ggcgggcgcg gcgggtcatg atcagccatt
    10921 gcggcgcggt cagcagcttg ggcagcgcca gcgaccaggg atcgggccag atcagcagca
    10981 gcagcgcggt gaggatatga tcggcgatcg cctgcgcgcc ggaaatggcg gccagctggc
    11041 tggtgcgccc ctcgcgcagg gccaggtgat attgcacatg cccgcccgcc atgaagggat
    11101 agaccagcgc cagcacggca aggatctgtg ccgccaccgg ccggtgggca aacagcgcca
    11161 ggcccgccgc tgccagccat tgcacgccca tcaggcccat cgtccacagc cagctgaggc
    11221 ggttggcggt gttgcaggtg gcggccagct cgtcgtccgg cacggccacg atcttctggc
    11281 cggtgccggt gcgttccagc acgcgcacga tctcgaagat ggtgagcgcc agcgccgcct
    11341 cgccaacgat atcgggcgcc agccgccggg cgatcaccac ggtggccacc agccgcacca
    11401 cgcgcgtggc cagttccgcc ccgccaaagg cgcccaagcc gcgcgccagc gggccatagc
    11461 cgccacccag acgccggtgt accaaggctt tgaggcgagc gatcacgcgg ggcaactcct
    11521 ggaagggcaa gggctcatgg gggcatgatc caagaaccgt gccatatccc gccagcctta
    11581 agattctgcc acatcggcag gaaatacagc gaggcaggca cgggtttttc gcaaattgcc
    11641 gtcgcaaatt gcaaagggcg ggcaggctgc gggtgcaaaa tgaaaagggc gccgggtgcg
    11701 ccttgcgcga ccaccggcgc ccttcgaagg cagcggcggg gcgccggccc cgccagttgg
    11761 cgtcaggcca cggcggcgcg cggcaggctg gccgcttcca gcgccgcgtg gatcgccgcg
    11821 aggaactggt cggtcgcccg ttcccacgag aagcgcgcgc caaagctggc cgcggcctcc
    11881 cggtccaccg ccagcgcgcg ggtgatcgcc gcgctcagat cctcgtcgag cgcgcccacc
    11941 ggcccgggcg cctcgccctc atggccgcgc ccgtcgtggc cgatgatgtc gagcgggccg
    12001 ggcaccggat aggccgccac cggcaccccg caggacaggg cctcgatcac caccagcccg
    12061 aacgtatccg tgcggctggg aaagacgaag caatcggccg tgcgataggc cgctgccagc
    12121 cgctcgccag ccagcgcgcc gaggaaatgc acccccggat agcgccgctg catatcggcc
    12181 agcgccgggc catcgcccac caccaccttg gtgcccggca gatccgcatc gagaaaggcc
    12241 tcgatgttct tttccggcgc cacccggcca acattgagca ggatggggcg cggcagatcg
    12301 gccatttccg gcagcaaagc gcccaccggg gcgaacaggc tgcggtctat cccgcggctc
    12361 cagcggtggg tgtgggccag gcccctctcg ttcagctcgc ccgcaaggct gggcgtggcc
    12421 accatcaccg cccggctggg cccgtggaaa tggcgcatca ccggccagaa ataatcggcc
    12481 ggaatgcccg tgcgcacggc cagatattcc ggaaagcggg tgtggaaggc gctggtgaac
    12541 ggcacccggc gcgaccggca ccagccgcgc gcgctccagc cgatggggcc ttcggtggcg
    12601 atatggacga tatccggccg gaagcgatcg agcatgcgcc gggccgaaaa gcgcggcgaa
    12661 accgccaggc ggatctggct gtagccgggc atgggcacgg tgaggaagcg ctcgggcgtg
    12721 atcagctcca cctcgatccc gcgcgcggtc agctcgttca ccgtggccga cagggtgcgc
    12781 accacgccgt tcacctgcgg aaaccaggca tcggtggcga tcgccaggcg caagggcgta
    12841 gcaggccgga aaggcgccgg agcaagcagg ttcatggcag ggtttccttg atcgcgcgcg
    12901 ccgctcatgc cggggtttgg gcggcagctt caggcgcgcg cagggcatga gccacgcgcg
    12961 attcctgcgc ccagtcgacg atggagatca cgcccgccac atcttccacc agcgcggtgc
    13021 agctttccac ccagtcgccg tcgttgtaat aggtgatgcc ctcgatctcg cggatctcgg
    13081 cggaatggat gtggccgcac accaccccgt ccacgccccg ctcgatggcg gcatgggcca
    13141 cggcttcctc aaagcggcca atgaattgca ccgcgttctt cacccgcttt ttcagatagg
    13201 cagagagcga ccagtagggc agcttcatcc gccggcggat gccgttgaac accacattca
    13261 cccgcatcat cgccgaatag gccttgtcgc ccaggaaggc cagccagcgg tgatagagca
    13321 ccaccgcatc gaaggcatcg ccatgggtaa ccagcaggcg ccggccatcc accgtctcgt
    13381 ggatgcattc cagcaccagt tccaccccgc cgaaagtgag gccggcatag gggcgcagca
    13441 tctcgtcgtg gttgccgcag acaaacacca cccgcgtgcc gcgatgggcc atcttgagga
    13501 tccggcggat cacctcatta tgagcatcgg gccaatacca cccgcgcgac aggcgccagc
    13561 catcgatgat atcgcccacc agatagatgg tgtcggtctc gatcgacttc aggaattcga
    13621 ccagcatcac cgcattgcag ccgcgcgtgc ccaggtggat atcggaaatc cacaccgtgc
    13681 ggtatttgcg cttg
//
LOCUS       DASRYE010000266        10430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_103312,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000266 DASRYE010000000
VERSION     DASRYE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103312"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFF98_13740"
     CDS             complement(<1..264)
                     /locus_tag="VFF98_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZU64740.1"
                     /translation="MSHNSAQGFAIVVDIGKTMSKVTLWSREGALLDRQVRPNAPCTQ
                     EGIARLDAGGIHQWLMDVLAGHAGCGAQYLVPVGHGAGFAALVA"
     gene            473..2578
                     /locus_tag="VFF98_13745"
     CDS             473..2578
                     /locus_tag="VFF98_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HZU64741.1"
                     /translation="MTTTTSRAALPFAVPASRWNEAEAASMSPEELLLYRSNLLGSDL
                     TVTNFGGGNTSAKLAATDPLTGAEVEVLWVKGSGGDIGSMKLDGFATLYQAKLLGLEA
                     HYSGPEDDDKMVGFLPHCTFNLNGRAASIDTPLHSLLPFAHVDHVHPDAIIALAASSG
                     GEAATKEIWGGKIGWLGWKRPGYTLGVMLRDYVAANPGIEGVMLAGHGIICWADTAKA
                     CYEHTVELIADAARYLNAKMAGKPAFGGEVVAPSADRAAVAADLMPRLRGLMTGARRK
                     LGHYSDDAEALEFVGSADFERLAALGTSCPDHFLRTKIAPLTLDPARLSDDAYLAEKI
                     ADYRAMYAAYYERCKRPNSPAMRDANPVVVLVPGVGRITFATDKTTARLAGEFYGNAI
                     NVMRGAEAIGDYIALDEQEAFDIEYWLLEEAKLQRMPAPKAQVGRVAFVTGGAGGIGA
                     ATAARFLKEGACVVLADRDGQAVEEVRAGFARQFGKDVVRAVTCDVTDEAQVQAAFDT
                     AAREFGGLDVVVANAGIASSASIEDTTVALWRKNYDVLAEGYFLTARAAWPLLKGMKA
                     QGGSAIVFIGSKNGVAAAAGASAYASAKAAANHLARCLALEGAPVGIRVNVVNPDAVI
                     RGSRIWDGDWRKERAGTHGIDPGAELEEHYRNRSMLKRDVLPEDIAEAAYFLGSEASA
                     KSTGNMINVDAGNVQAFTR"
     gene            2605..3897
                     /gene="rhaI"
                     /locus_tag="VFF98_13750"
     CDS             2605..3897
                     /gene="rhaI"
                     /locus_tag="VFF98_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HZU64742.1"
                     /translation="MTQLPISAELIAERNEAGLEALREDYHALGRKLARTGIDIDAIK
                     ARVAAFSVAIPSWGAGRGGTRFAKFPIAGEPTNIHEKLEDCAVIQQLSRATPRVSPHF
                     PWDKVSDYKALREEAASLGLGFDAVNSNTFQDQKDQPHTYANGSLSAQDAATRQQAID
                     HNIECIEIGRQLGSTDLTVWVGDGTNLPGQQDLGRSLDRYLEAASQIYAALPDDWRML
                     LEHKMFEPAFYSTVISDWGSSILAAQELGPKARCLVDLGHHAPNVNIEQIVARLHRFG
                     KLGGFHFNDSKYGDDDLDSGSINPHQLFLIFNELVEAELNPRDGFNPSYMIDQSHNVT
                     DPIESMLSSAETITSCFAKAVLVDRAALHAAHAANDTMMGFQALRRAYNVDVAPILAM
                     ARVEAGGALDVLATYRASQYRDRKAQERAAVGLGAGIV"
     gene            4014..7457
                     /locus_tag="VFF98_13755"
     CDS             4014..7457
                     /locus_tag="VFF98_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZU64743.1"
                     /translation="MTATTRTCALRAALAAASALTGAFAALPAAAQTAAPATTPADTS
                     LDAQFRDPPAEARPRVWWHWMNGNITQDGIAKDLAWMKAVGIGGAQTFDANLHTPQIV
                     PHRLVYMTPEWKAAFHFAASEADRLGLELAIAASPGWSETGGPWVSPEDGLKKLVWSE
                     ARLAPGQRLAGPLAKPPVVTGPFQSLPKQLGLDDLVADGKPPVLPGAAGDIAVLAIPE
                     AAPADVVPAYATGAGTALDAARLTDADLATGVAVPRGGADAPAAVAITYAAPRAIRSA
                     TIFIAHASVMFGGTVVDPVLEASEDGTHWREIAHFPMAEVPTTISFAPVVAARFRVVL
                     HPLQPAQANMGAPAPGIVAPDLFGALLGGAGQPWDVRQLTLSDAPAVDRFETKAGFSI
                     AMDYYALGQPGDGAAGPAPARVIDITSHLKADGTLDWQAPALPRGQQWRIVRLGWSLL
                     GTTNHPASPEATGLEVDKFDGEAVQRYMQHYLGLYRDAAGPGLIGAHGVRALVTDSIE
                     VGAANWTPRMVAQFKARRGYDPTPWLPVLAGILVGSRAEADSFLYDYRRTLAELMASE
                     HYGTVANMAHAAGLKVYGEALEDKRPSLGDDMAMRSHTDVPMSAMWTYPRGGQPQPSY
                     LADIKGAASVAHIYGQNLVAAESMTSALNYWADSPRTLKHVIDMEFVTGVNRPVVHTS
                     VHQPVDDKVPGLSLLIFGQFFNRHESWAPLARAWVDYISRNSLLLQKGVNVADVGYFY
                     GEEAPLTGLYGLKPVADAPTTHAYDFVNADALAGALKNEGAELVTPGGARYKALYLGG
                     SSRRMTLPALRRIADLAAGGATIIGLAPEGTPSLSDSDAAAYKALVAQLWPAGGESEA
                     LVGKGRVIASADVEQGLARAGIASDVKLEGASAGADIGFIHRRWAGGEDYFLVNKSEK
                     PEVVEAHFRITGKAPELFHAETGTSEAVSYRILGGETVVPLSLAAEEAVHVVFRKPAL
                     ADGLALKKLAPAPIATLAGPWDVAFQPGRGAPARATLAELKPLNENADPAIRYFSGLA
                     TYSKLFAAPAGWHKGQPLWLNLGDLRELAEVRVNGQSLGTSWHAPYRLDISGAVKPGQ
                     NRLEVTVANLWVNRLIGDAQPGVKEKITFTPLPTYRADAPLVASGLIGPVVLEGAAKA
                     R"
     gene            7468..8364
                     /locus_tag="VFF98_13760"
     CDS             7468..8364
                     /locus_tag="VFF98_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64744.1"
                     /translation="MIDPRALRTFHEVCRAGSISAAARVLNIAQPSVSNAIALLEARL
                     GARLFERTRQGIVLTPAGEALRARAAILDHLLRDAEAEVAAAVQGVAGPLRVGGTPGA
                     LVSLLPRALGVLEAAHPGLSLNVLERPDGELTDLLREGAIDLACVTTDIEAPPADMAE
                     RTFARDPFALIVGPANAALPERLSLAMAEDLAWVLPEARGGFRRQVDALFLAAGVPAP
                     RNVVRCDSLLTTKAIVRGGARVTILPMHVAKAELSIGVLRAIRLEEARFERSVGLRWV
                     KGRPLSPLAGAMMAALESMHSQ"
     gene            8451..9680
                     /locus_tag="VFF98_13765"
     CDS             8451..9680
                     /locus_tag="VFF98_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU64745.1"
                     /translation="MATLFTNIRIFDGTPNPARAGQVLVEGNEIARVAYAPDAIEAAG
                     ASVVDGKGGTLMPGMVEAHTHLTWASSVEKIYHAFILPPEELEVAATRNARVLLDHGF
                     TSLYSAGALGDGIEVRLAAKIAAGEVPGPRLVPSTLERSPEGAEGVETGDVFNGRGPE
                     AMRAFMAYCAKEGIGSVKLVISGEDALKPGSHADLLYTAEEMDAAREAAREHGLWIAT
                     HAYTDAAVELALRAGARIIYHASYIGEETIARLAACKDEVFYGPGCGVSVAALEASPP
                     PHIDMSHMKASAAERMALEGRLVPKLKAAGVRVLIGGDYGFPFNPNGRNARDLQHFVD
                     YFGYTPLEALAAATSLGGELMGLKVGQLRAGYLADLLLVDGDPTQDVAILQDAGRLRA
                     IMKGGVFHKAPAEAAVA"
     gene            9685..>10430
                     /locus_tag="VFF98_13770"
     CDS             9685..>10430
                     /locus_tag="VFF98_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZU64746.1"
                     /translation="MFDVAIIGCGPVGALAANLLGRAGLKVVVLEKELSHYPLPRAVH
                     LDHEMMRLFQSAGVIDAVLPDMVDTDGHLHIGSAHQVIRYMGTVGMARPFGWSNDWFF
                     YQPELEAHLRAGFAGLANVTLHLGAEFTGLAQAGEVVTVDYLQGGAAAQVQARWVIAA
                     DGSRSPVRKALGVALDDLKFEEPWLVVDAEVDGPVAFPEIAGVPKEADLQRLSVMVCD
                     PARPATVVPGRGNHRRWELMLLPGEEDAAM"
BASE COUNT         1650 a         3452 c         3630 g         1698 t
ORIGIN      
        1 agccaccagc gcggcaaagc cggcgccgtg gcccaccggc accagatatt gcgcgccgca
       61 gccggcatgg ccggccagca cgtccatcag ccactggtga atgcccccgg catccagccg
      121 ggcgatgccc tcttgcgtgc aaggggcatt gggccgcacc tgccggtcga gcagcgcgcc
      181 ctcgcgtgac cacagcgtca ccttcgacat cgtcttgccg atatcgacga cgatcgcaaa
      241 cccttgcgcg ctgttgtggc tcattggccc ggccctgctc cctgtgtgtc ctgcttgcgc
      301 agggctcccc gccttgtgtt gcgggtgcga aacgattgtg catgtgcgaa attgatcgtt
      361 tgcgttgccg cattatgatc gattgtgatt atgtcctatc gaatccgatt gccaatggaa
      421 agcggaatcg gtacacggcc cgataaacac accacccggg agcatccccc acgtgaccac
      481 taccacatcg cgcgccgccc ttccctttgc cgtgcccgcc agccgctgga acgaagccga
      541 agccgccagc atgagccccg aagagctgct gctctatcgc tcgaacctgc tcggttccga
      601 tctcaccgtc accaatttcg gcggcggcaa cacctctgcc aagctggccg ctaccgatcc
      661 gctgaccggc gccgaggtgg aggtgctgtg ggtgaagggc tcgggcgggg atatcggctc
      721 gatgaagctc gatggttttg ccacgctcta tcaggccaag ctgctggggc ttgaggccca
      781 ttacagcggc ccggaggatg acgacaagat ggtcggcttc ctgccgcact gcaccttcaa
      841 cctcaacggc cgtgccgctt ccatcgacac gccgctccat tcgctgctgc cctttgccca
      901 tgtcgatcat gtgcaccccg atgcgatcat cgcgctggcg gccagcagcg gcggcgaggc
      961 ggccaccaag gagatctggg gcggcaagat cggctggctg ggctggaagc gcccgggcta
     1021 cacgctgggc gtgatgctgc gcgattatgt ggcggcgaac ccgggcatcg agggcgtgat
     1081 gctggccggc catggcatca tctgctgggc cgacacggcc aaggcctgct acgagcacac
     1141 ggtggagctg atcgccgatg cggcccgcta tctcaacgcc aagatggccg gcaagcccgc
     1201 ctttggcggc gaagtggtgg cccccagcgc cgatcgcgcg gcggtcgcgg cggatctcat
     1261 gccgcgcctg cgcgggctga tgaccggcgc gcgccgcaag ctgggccatt attccgatga
     1321 tgccgaggcg ctggaatttg tcggcagcgc cgatttcgag cggctggctg cgctgggcac
     1381 ctcctgcccg gatcacttcc tgcgcaccaa gatcgccccg ctgacgctgg acccggcgcg
     1441 gctgagcgat gatgcctatc tggccgagaa gatcgccgac taccgcgcca tgtatgccgc
     1501 ctattacgag cgctgcaagc ggcccaacag cccggcgatg cgtgatgcca acccggtggt
     1561 ggtgctggtg cccggcgttg gccgcatcac ctttgccacc gacaagacca ccgcgcgcct
     1621 ggccggcgag ttctatggca atgccatcaa cgtgatgcgc ggcgccgagg cgattggcga
     1681 ctatatcgcg ctcgacgagc aggaagcctt cgacatcgaa tactggctgc tggaagaggc
     1741 caagctgcag cgcatgccgg cgcccaaggc gcaggtgggc cgggtggctt tcgtcacggg
     1801 cggggcgggc ggcattggcg cggccaccgc ggcgcgcttc ctcaaggagg gcgcctgcgt
     1861 ggtgctggcc gaccgcgacg ggcaggccgt ggaagaggtg cgcgccggct ttgccaggca
     1921 gtttggcaag gatgtggtgc gcgcagtgac ctgcgatgtg accgatgagg cccaggtgca
     1981 ggccgctttc gataccgccg cgcgcgaatt tggcgggctg gatgtggtgg tggccaatgc
     2041 cggcatcgcc tcttccgcca gcatcgagga caccaccgtg gccctgtggc gcaagaatta
     2101 cgacgtgctg gccgagggct atttcctcac cgctcgcgcc gcctggccgc tgttgaaggg
     2161 catgaaggcg cagggcggca gcgcgatcgt cttcatcggc tcgaagaatg gcgtggctgc
     2221 tgctgccggt gccagcgcct atgcctcggc caaggcggcg gccaatcacc tggcccgctg
     2281 cctggcgctg gaaggggcgc cggtgggcat ccgcgtcaat gtggtgaacc ccgatgcggt
     2341 gatccgcggc agccgcatct gggatggcga ctggcgcaag gagcgcgccg gcacccacgg
     2401 cattgacccc ggcgccgagc tggaggagca ttaccgcaac cgctcgatgc tgaagcgcga
     2461 cgtgctgccc gaagacattg ccgaggccgc ctatttcctc ggcagcgagg cttccgccaa
     2521 atcgaccggc aatatgatca atgtcgatgc cggcaatgtg caggccttca cgcgctgaac
     2581 cgcgacagac gggagaggat tatcatgacc caactgccca tttccgccga gctgatcgcg
     2641 gaacgcaacg aagccggcct tgaggccctg cgcgaggatt accatgcgct gggccgcaag
     2701 ctggcgcgca ccggcatcga tatcgatgcc atcaaggccc gcgtggccgc tttctcggtg
     2761 gcgatccctt cgtggggggc ggggcgcggc ggcacccgct ttgccaagtt ccccattgcg
     2821 ggcgagccca ccaacatcca cgagaagctg gaagactgcg ccgtcatcca gcagctcagc
     2881 cgcgcaacgc cgcgggtgtc gccgcatttc ccgtgggaca aggtgagcga ttacaaggcc
     2941 ctgcgcgagg aagcggccag ccttggcctg ggcttcgatg cggtgaacag caacaccttc
     3001 caggatcaga aggaccagcc ccacacctat gccaatggct cgctgagcgc gcaggatgcc
     3061 gccacccgcc agcaggccat cgaccataac atcgaatgca tcgagatcgg ccgccagctg
     3121 ggctccaccg atctcaccgt gtgggtgggc gatggcacca acctgcccgg ccagcaggat
     3181 ctgggccgca gccttgatcg ctatctggaa gcggccagcc agatctatgc cgccctgccg
     3241 gacgactggc gcatgctctt ggaacacaag atgttcgagc cggccttcta ttcgaccgtc
     3301 atcagcgact ggggctcctc gatcctcgcc gcgcaagagc tgggccccaa ggccaggtgc
     3361 ctggtggatc tgggccatca tgcgcccaat gtgaacatcg agcagatcgt ggcgcggctg
     3421 caccgcttcg gcaagctggg cggcttccac ttcaacgaca gcaaatatgg cgacgacgac
     3481 ctcgattcag gttcgatcaa cccgcaccag ctgttcctca tcttcaacga gctggtggaa
     3541 gccgagctga acccgcgcga cgggttcaac cccagctaca tgatcgacca gtcgcacaat
     3601 gtcaccgatc cgatcgagag catgctctcc tcggccgaga cgatcaccag ctgctttgcc
     3661 aaggccgtgc tggtggaccg cgcggcgctg catgccgccc atgccgccaa cgacacgatg
     3721 atgggcttcc aggcactgcg ccgggcctat aacgtggatg tggcccccat cctcgccatg
     3781 gcgcgggtgg aggcgggcgg cgcgctcgac gtgctggcca cctatcgcgc cagccaatac
     3841 cgcgaccgca aggcgcagga acgcgcagcc gtggggctgg gcgccgggat cgtctgaccg
     3901 aagggcgcca tgccggcgct gcatcacaga tcggggggct ggccgccggc gcatatccgc
     3961 aagggatgcg ccggcgggca agcccccttt gccttatcga tggggagaga aacatgaccg
     4021 ccaccacgcg cacctgtgcc ctgcgcgcag cccttgccgc tgccagtgcc cttaccggcg
     4081 cctttgccgc cctgccggcc gccgcccaga ccgccgcgcc ggccaccacg ccggccgaca
     4141 ccagccttga cgcccagttc cgcgatccgc ccgccgaggc gcgcccgcgc gtgtggtggc
     4201 actggatgaa cggcaacatc acccaggatg gcatcgccaa ggatcttgcc tggatgaagg
     4261 cagtgggcat cggcggcgcg cagaccttcg atgccaatct ccacacgccg cagatcgtgc
     4321 cgcaccggct ggtctacatg acgccggaat ggaaggcggc cttccacttc gccgccagcg
     4381 aggccgaccg gctggggctg gagctggcga tcgccgcctc gccgggctgg tcggaaacgg
     4441 gcgggccctg ggtgagcccg gaagacgggc tgaagaagct ggtgtggagc gaggcgcggc
     4501 tggcgccggg ccagcgcctg gccggcccgc tggccaagcc gccggtggtg acaggcccgt
     4561 tccagtcttt gcccaagcag ctggggctgg atgatctggt ggccgatggc aagccgccgg
     4621 tgctgccggg cgcggccggc gatatcgccg tgctggccat tcccgaagcc gcgcccgccg
     4681 atgtggtgcc cgcctatgcc acaggcgccg gcaccgcgct ggatgccgcg cggctgaccg
     4741 atgccgacct tgccaccggc gttgcggtgc cgcgcggcgg ggccgatgcg ccggccgcgg
     4801 tggccatcac ctatgccgcc ccgcgcgcca tccgttcggc cacgatcttc atcgcccatg
     4861 ccagcgtgat gtttggcggc acggtggtcg atccggtgct ggaagccagc gaggatggca
     4921 cccactggcg cgagatcgcc catttcccca tggccgaagt gcccaccacc atcagctttg
     4981 ccccggtggt ggcggcccgt ttccgcgtgg tgctgcatcc gctgcagccg gcgcaggcca
     5041 atatgggcgc gccggcgccg ggcatcgttg cgcccgatct gttcggcgcg ctgctgggcg
     5101 gggcgggcca gccctgggat gtgcgccagc tcaccttgtc ggatgcgccg gcggtggacc
     5161 gctttgaaac caaggccggc ttttccatcg ccatggacta ttacgcgctg ggccagccgg
     5221 gcgatggcgc ggcaggccct gcgcccgcgc gggtgatcga catcaccagc cacctcaagg
     5281 ctgatggcac gctggactgg caggcaccgg ccctgccgcg cggccagcag tggcgcatcg
     5341 tgcggctggg ctggtcgttg ctgggcacca ccaaccaccc cgccagcccc gaggcgaccg
     5401 gccttgaggt ggacaagttc gatggcgagg cggtgcagcg ctacatgcag cattatctcg
     5461 gcctttaccg cgatgcggca gggccggggc tgatcggcgc ccatggcgtg cgcgcgctgg
     5521 tgaccgacag catcgaggtg ggcgcggcca actggacgcc caggatggtg gcccagttca
     5581 aggcacggcg cggctatgat cccaccccct ggctgccggt gctggcgggc attctggtgg
     5641 gcagccgggc cgaggcggac agcttcctct acgactaccg ccgcacgctg gccgagctga
     5701 tggccagcga acattatggc acggtggcca atatggccca tgcggccggc ctcaaggtct
     5761 atggcgaggc gctggaagac aagcgcccca gccttggcga cgacatggcc atgcgcagcc
     5821 acaccgatgt gccgatgagc gccatgtgga cctatcccag gggcggccag ccgcagcctt
     5881 cctatctggc cgacatcaag ggcgcggcca gcgtggccca tatctatggc cagaatctgg
     5941 tggcggccga aagcatgacc tcggcgctga actactgggc tgattcgccg cgcacgctca
     6001 agcatgtgat cgacatggaa tttgtcaccg gcgtcaaccg gccggtggtg cacacctcgg
     6061 tgcaccagcc ggtggacgac aaggtgccgg gcctttcgct gctgatcttc ggccagttct
     6121 tcaaccggca tgagagctgg gcgccgctgg cccgcgcctg ggtggattac atcagccgca
     6181 attcgctgct gctgcaaaag ggcgtgaatg ttgccgatgt cggctatttc tatggcgagg
     6241 aagcgccgct gacggggctt tatggcctga agccggtggc cgatgcgccc accacccatg
     6301 cctatgattt cgtcaatgcc gatgcgctgg caggggcgct gaagaacgag ggcgccgagc
     6361 tggtgacgcc gggcggagcg cgctacaagg cgctgtatct gggcggatcg agccggcgga
     6421 tgacgctgcc ggcgctgcgc cggatcgccg atctggccgc aggcggggcc accatcattg
     6481 gcctggcgcc cgagggcacg cccagcctct ccgacagcga tgcggcggcc tataaggcgc
     6541 tggtggcgca gctgtggccg gctggcggtg agagcgaggc actggtcggc aagggccggg
     6601 tgatcgcctc ggccgatgtg gagcaagggc tggcccgcgc cggcattgcc agcgatgtga
     6661 agctggaagg cgcctcggcc ggcgcggata tcggctttat ccaccgccgc tgggccgggg
     6721 gcgaggatta cttcctcgtc aacaagagcg aaaagcccga agtggtggag gcccatttcc
     6781 gcatcaccgg caaggcgccc gaactgttcc acgccgaaac cggcaccagc gaggcggtgt
     6841 cctatcgcat cctcggcggg gaaacggtgg tgccgctcag cctggcggcg gaagaggcgg
     6901 tgcatgtggt gttccgcaag ccggcgctgg ccgatgggct ggcgctgaag aagctggcgc
     6961 cggcgcccat cgccacgctg gcgggcccgt gggacgtggc cttccagccc ggccgcggcg
     7021 cgccggcccg cgccaccctt gccgagctga agccgctgaa cgagaatgct gatccggcga
     7081 tccgctattt ctcgggcctg gcgacgtata gcaagctgtt tgccgcgcct gccggctggc
     7141 acaagggcca gccgctgtgg ctgaacctgg gcgatctgcg cgagctggcc gaggtgcggg
     7201 tgaacgggca gagcctgggc accagctggc acgcgcccta tcggctggat atttcgggcg
     7261 cggtgaagcc gggccagaac cggctggagg tgacagtggc caacctgtgg gtgaaccggc
     7321 tgatcggcga tgcccagccg ggggtgaagg agaagatcac tttcacgccg ctgcccacct
     7381 atcgtgccga tgcgccgctg gtggcatcgg gcctgatcgg cccggtggtg ctggaagggg
     7441 cggccaaggc gcgctaaaca cccccgcatg atcgacccgc gcgcccttcg cacatttcac
     7501 gaggtttgcc gcgcgggctc gatcagcgcg gcggcgcggg tgctcaatat cgcccagccg
     7561 tctgtttcca atgcgattgc gctgctggag gcgcggctgg gcgcccgcct gttcgagcgc
     7621 acccgccagg gcatcgtgct gacgcccgcc ggcgaggcgc tgcgcgcgcg ggcggctatt
     7681 ctcgatcatc tgctgcgcga tgccgaggcc gaggtggcag ccgccgtgca gggcgtggcc
     7741 ggccccttgc gggtgggcgg cacgccgggc gcgctggtca gcctgctgcc gcgcgcgctg
     7801 ggggtgctgg aggcggccca tccgggcctt tcgctcaacg tgctggaacg gcccgatggc
     7861 gagctgaccg acctcttgcg cgagggcgcc atcgaccttg cctgcgtgac caccgacatc
     7921 gaggcgccgc cggccgacat ggccgagcgc accttcgcgc gtgatccttt cgcgctgatc
     7981 gttggcccgg ccaatgcggc gctgcccgag cgcctctctc ttgccatggc agaggatctg
     8041 gcctgggtgc tgcccgaggc gcgcggcggc tttcgccggc aggtggatgc gctgttcctg
     8101 gccgcgggcg tgccggcgcc gcgcaatgtg gtgcgctgcg attcgctgtt gaccaccaag
     8161 gccatcgtgc gcggcggcgc gcgggtgacg atcctgccga tgcatgtggc caaggcggaa
     8221 ctttcgattg gcgtgctgcg ggcgatccgg ctggaagagg cccgtttcga gcggtctgtg
     8281 ggcctgcgct gggtgaaggg gcggccgctg tcgccgctgg caggcgcgat gatggcggcg
     8341 ctggagagca tgcatagcca atagctatgg aataatagct aacccatatt ttacctgagg
     8401 cccgccgctt ggcagatagg ccccaagcca acaataaggg agagccgggc gtggcaacgc
     8461 tctttaccaa catccgcatt ttcgatggca cgcccaatcc ggcgcgcgca ggccaggtgc
     8521 ttgtcgaggg caacgagatt gcccgcgtgg cctatgcgcc cgacgcgatc gaggcggccg
     8581 gcgccagcgt ggtcgatggc aagggcggca cgctgatgcc cggcatggtg gaggcgcaca
     8641 cgcatctcac ctgggcctcc tcggtggaaa agatctatca cgccttcatc ctgccgcccg
     8701 aggaactgga agtggccgcc acgcgcaatg cccgcgtgtt gctcgaccat ggttttacca
     8761 gcctctattc ggccggcgcg ctgggcgatg gcatcgaggt gcgcctcgcc gcgaagattg
     8821 ccgccggcga agtgcccggc ccgcgcctgg tgccctccac gctggagcgc agcccggaag
     8881 gggccgaggg cgtggaaacc ggcgatgtgt tcaacggccg cgggcccgag gcgatgcgcg
     8941 ccttcatggc ctattgcgcg aaggaaggga tcggctctgt caagctggtc atttccggcg
     9001 aggatgcgct gaagccgggc agccatgccg acctgctcta caccgccgag gaaatggatg
     9061 ccgcccgcga ggccgcccgt gaacacggcc tgtggatcgc cacccatgcc tatacggatg
     9121 cggcggtgga gctggccctg cgcgccggcg cccgcatcat ctatcacgcc agctatatcg
     9181 gcgaggaaac catcgccagg ctggccgcct gcaaggacga ggtgttctac ggccccggct
     9241 gcggcgtttc cgtggcggcg ctggaagcca gcccgccgcc gcacatcgac atgagccaca
     9301 tgaaggcgtc ggctgccgag cgcatggcgc tggaaggccg gctggtgccg aagctgaagg
     9361 cggccggcgt gcgcgtgctg atcggcggcg attatggctt cccgttcaac cccaacggcc
     9421 gcaatgcccg cgatctccag catttcgtcg attatttcgg ctataccccg ctggaagcgc
     9481 tggccgcggc cacgtcgctg ggcggcgagc tgatggggct gaaagtgggc cagctgcgcg
     9541 ccggctatct tgccgatctg ctgctggtgg atggcgatcc gacgcaagat gtggcgatcc
     9601 tgcaggatgc ggggcgcttg cgggcaatca tgaagggcgg tgtcttccac aaggcgcctg
     9661 ccgaggctgc ggtggcataa aaccatgttc gatgttgcga ttatcggctg cggcccggtg
     9721 ggtgcgctgg cagcgaacct gcttggccgc gcgggcctca aggtggtggt gctcgaaaag
     9781 gagctgagcc attatccgtt gccgcgcgcg gtccatctcg atcacgagat gatgcgcctg
     9841 ttccagtcag ccggcgtgat cgatgccgtg ctgcccgaca tggtggacac cgacgggcac
     9901 ctccatatcg gcagcgccca tcaggtgatc cgctatatgg gcacggtggg catggcccgg
     9961 ccctttggct ggtcgaacga ctggttcttc tatcagcccg agctggaagc gcatctgcgc
    10021 gcaggctttg ccggcctggc caatgtgacg ctgcatctgg gcgcggaatt caccggcctt
    10081 gcccaggcgg gcgaggtggt gacggtggac tacctgcagg gcggcgctgc ggcgcaggtg
    10141 caggcgcgct gggtgatcgc cgccgatggc tcgcgcagcc cggtgaggaa agcgctgggc
    10201 gtcgcgctcg atgatctgaa gtttgaggaa ccctggctgg tggtcgatgc cgaggtggat
    10261 gggccggtgg ccttccccga gatcgccggc gtgcccaagg aggctgatct ccagcgcctg
    10321 tcggtgatgg tgtgcgatcc ggcgcgcccg gcaacggtgg tgcccggccg cggcaaccat
    10381 cgccgctggg aactgatgct gctgcccggc gaagaggatg ccgcgatgat
//
LOCUS       DASRYE010000267         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_63198,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000267 DASRYE010000000
VERSION     DASRYE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63198"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFF98_13775"
     CDS             <1..548
                     /locus_tag="VFF98_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64747.1"
                     /translation="AGTVAAITIGAATITAPDPARVSGEEKKAAIAAFTAQVVGKAAT
                     PAKPAEGGKPAVEAKPAVVGALSKVGYPAKADPAQINKPKVVALLFYLVLLVTMVYGP
                     IAAMLVELFPSRIRYSSMSLPYHIGNGWLGGLLPAIGFAMVAANGDIYFGLWYPVVAA
                     AACVVFGLLFLPETFRRNIDN"
     gene            645..1166
                     /locus_tag="VFF98_13780"
     CDS             645..1166
                     /locus_tag="VFF98_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZU64748.1"
                     /translation="MTVDAAPLAKLPTRSGLTIAVRLATEADEAALEALFADVTEADR
                     RFRFLAASTHVGHEQLEPLIHADHFRTESYLAFSEETGELVASAMLACDGKLDTAEVA
                     VAIRADHKGRGVGWALLDLLGEEARRRGVRRVISIESRDNHEAIELEREKGFEPEAFD
                     GDPTLVVLSKTFR"
     gene            1204..3099
                     /locus_tag="VFF98_13785"
     CDS             1204..3099
                     /locus_tag="VFF98_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU64749.1"
                     /translation="MSGTYHQAWQNSIADPEQFWLDAAGAVEWVSPPDKGFDREEGWF
                     AGAALNTSHNCLDRHVAAGRGDTLALIHDSPATGQTSRFTYAQLLAEVEAVAAMLVRL
                     GVEKGDRVIVYMPMVPETVFAMLACARLGAVHSVVFGGFAAHELAKRIDDAKPRLVLT
                     ASCGVEGSRLVPYMPMVAEALRLASHPVAGVVVHQRPQLTAQLDPARDHDWAALRAAT
                     AADPRPAPVAVAASDPLYILYTSGTTGTPKGVVRDNGGHATALSWSMANIYGIGAGDV
                     FWAASDVGWVVGHSYIVYAPLLVGATTVLFEGKPVGTPDAGTFWRSIVAHGVKVFFTA
                     PTAIRAIRKEDPEGRFLAEIGTGAAQAIFLAGERADPETIAWLEEKSGLPVIDHWWQT
                     ELGWPAIATCHALGDTRRRRGSAGFPVPGWQFGVLGDDGQVLEAGQSGFVAIRAPLPP
                     GAFRTLWNNKAGYEKNFAAFPGWYETGDAGHFDAEGFLHIMGRTDDIINVAGHRLSTG
                     QMEQIVAAVDGVAECAVIGADDAIKGMIPVAFVVPRAGAEGDASLVARVVAAVRADLG
                     AVAALKLAYVVPQLPKTRSGKILRNLLRRLANGQEWQVPPTIDDPAAPGLIAAALAAA
                     EGTRAAG"
     gene            complement(3119..>4296)
                     /locus_tag="VFF98_13790"
     CDS             complement(3119..>4296)
                     /locus_tag="VFF98_13790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU64750.1"
                     /translation="GLLASTAIGVFETSRLPVLFVLALPEMLVAFRLGWLGTQLGIVL
                     LALIGSIMTAHGFGPISLASHDAPTRALFFQFFLATQVLLILPVVASLAASASVLNAL
                     RESEQSVQLLAEEAAVLLLGFAADGTCLKVVGNVGAMLGIGERALLGRDLGALSPINR
                     TPLLELYAQALAQPETVLSLEFSSPLRPGRWLEANLRAPETLAGPQGSSVLVVLQDVT
                     DRHDAQLALLRAAETDELTGLLNRAAFMSRLEAALRDERATRIHLAIFDVDRFKVIND
                     SAGHAMGNAVLQAVAERMAGAVCAPDLLCRMGGDEFLLMLECASLAEAETRCQAVLNA
                     LHGQPIDLPTGDTLSVRVSCGLAGGGRGQSSEQMLHRADAAVYAAKRAGRNRIAAVA"
BASE COUNT          688 a         1443 c         1479 g          686 t
ORIGIN      
        1 ccgccggcac ggtggccgcc atcaccatcg gcgctgccac catcaccgcg cccgatccgg
       61 cccgggtgag cggcgaggag aagaaggccg ccatcgctgc cttcacggcc caggtggtgg
      121 gcaaggcggc aacgcctgcc aagcctgccg aaggcggcaa gcccgcggtg gaagccaagc
      181 ctgccgtggt cggcgcgctc tcgaaggtgg gctatccggc caaggcggat ccggcgcaga
      241 tcaacaagcc caaggtggtg gcgctgctgt tctacctcgt gctgctggtg acgatggtct
      301 acggcccgat cgcggccatg ctggtggagc tgttccccag ccgcatccgc tattcctcca
      361 tgtctttgcc ctatcacatc ggcaatggct ggctgggcgg cctgctgccg gcgatcggct
      421 ttgccatggt ggcggccaat ggcgacatct attttggcct gtggtatcct gtcgttgcag
      481 ccgcggcctg tgttgtcttc ggcttgctgt tccttcctga aaccttccgc cgcaatatcg
      541 acaactgatg ttgcaaaaaa cgggtgccgc gccttgcccc aaatcaaggc gcggcatccg
      601 ttttgtggca gaaacccggc aaatttcatc atggagtgac tgtcatgact gtcgatgccg
      661 ccccgcttgc caagcttccc acccgttccg gcctgacgat cgccgtgcgc ctggccaccg
      721 aggccgacga ggccgcgctg gaagcgctgt tcgccgatgt gaccgaggcc gatcgccgct
      781 tccgcttcct ggccgccagc acccatgtcg gccatgagca gctggaaccg ctgatccacg
      841 ccgatcattt ccgcaccgaa agctatctcg ccttttccga ggaaaccggc gagctggtcg
      901 cctcggccat gctggcctgc gatggcaagc tggatacggc cgaagtggcc gtggccatcc
      961 gcgccgatca caagggccgg ggcgtgggct gggccctgct cgacctcctg ggcgaggaag
     1021 cccgccgccg cggcgtgcgc cgggtgatct cgatcgagag ccgcgacaac cacgaggcga
     1081 tcgaactgga gcgcgaaaag ggcttcgaac cggaagcctt cgacggcgat ccgaccttgg
     1141 tcgtgctttc caagaccttc agataaaagc acacccaatg aagtaataat aaaggggaag
     1201 atgatgagcg gcacctacca ccaggcctgg cagaacagca ttgccgatcc tgagcaattc
     1261 tggctggatg cagccggggc cgtggagtgg gtgtcgcccc ccgacaaggg atttgaccgc
     1321 gaggaaggct ggtttgcggg cgcggcgctc aacaccagcc acaattgcct cgatcgccat
     1381 gtggccgccg gccggggcga cacgctggcc ctcatccacg acagccccgc caccggccag
     1441 accagccgct tcacctatgc ccagctgctg gccgaggtgg aggcggtggc cgccatgctg
     1501 gtgcggctgg gggtggaaaa gggcgatcgg gtgatcgtct acatgccgat ggtgcccgaa
     1561 accgtgtttg ccatgctggc ctgcgcgcga ctgggggcgg tgcattcggt ggtgttcggc
     1621 ggctttgccg cgcatgaact ggccaagcgg atagacgatg ccaagccccg gctggtgctc
     1681 accgcctctt gcggggtgga gggcagccgg ctggttccct atatgccgat ggtggcagag
     1741 gcgctgcgcc tggccagcca tccggtggca ggcgtggtgg tgcaccagcg cccgcaactg
     1801 acagcacagc ttgatcctgc ccgcgaccat gactgggcgg ccctgcgcgc ggccaccgcg
     1861 gccgatccgc gcccggcgcc ggtggccgtg gccgccagcg atccgctcta tatcctctac
     1921 acctccggca ccaccggcac ccccaagggc gtggtgcgcg acaatggcgg ccacgccacg
     1981 gcgctgagct ggagcatggc caacatctat ggcattggcg cgggcgatgt gttctgggcg
     2041 gccagcgatg tgggctgggt cgtgggccat tcctatatcg tttatgcgcc gctgctggtg
     2101 ggggccacca ccgtgctgtt cgagggcaag ccggtgggca cgccggacgc cggcaccttc
     2161 tggcgcagca tcgtggcgca tggggtgaag gtgttcttca ccgcgcccac cgccatccgc
     2221 gccatccgca aggaggatcc ggaggggcgt ttcctggccg agatcggcac gggcgcggcc
     2281 caggcgatct tcctcgccgg cgagcgagca gatccggaaa ccatcgcctg gctggaggaa
     2341 aaatcgggcc tgccggtgat cgatcactgg tggcagacag agctgggctg gccggccatc
     2401 gccacctgcc atgcgctggg cgatacccgc cggcgccggg gcagcgcggg ctttccggtg
     2461 cccggctggc agtttggcgt gctgggcgat gatggccagg tgctggaagc ggggcagagc
     2521 ggctttgtcg ccatccgtgc gccgctgccg ccgggggcct tccgcacgct gtggaacaac
     2581 aaggccggct atgagaagaa tttcgccgcc ttccccggct ggtatgaaac cggcgatgcc
     2641 ggccattttg atgccgaggg cttcctccac atcatgggcc gcacggatga catcatcaat
     2701 gtggcgggcc atcgcctgtc taccgggcag atggagcaga tcgtcgcggc ggtggatggc
     2761 gtggccgaat gcgcggtgat cggcgcggat gatgccatca agggaatgat cccggtggct
     2821 ttcgtggtgc cgcgcgcggg ggcggaaggt gatgcctcgc tggtggcgcg ggtggtggcg
     2881 gcggtgcgcg cggatctggg cgcggtggca gcgctgaagc tggcctatgt ggtgccgcag
     2941 ctgcccaaga cgcgttccgg caagatcctg cgcaaccttt tgcgccggct ggccaacggg
     3001 caggaatggc aggtgccgcc aaccatcgac gatccggccg cacccggcct gatcgccgct
     3061 gccctggccg cggccgaggg cacccgcgcg gccggctagc cgtgtgaccc cgggcgggtc
     3121 aggccaccgc ggcgatgcgg ttgcgcccgg cccgcttggc ggcataaacg gccgcatccg
     3181 cccggtgcag catctgctcg ctggattggc cgcgcccgcc gccggccagc ccgcagctca
     3241 cccgcacgct cagcgtgtcg ccggtgggca ggtcgatcgg ctggccgtgc agcgcattga
     3301 gcaccgcctg gcagcgggtt tccgcctcgg ccaggctggc gcattccagc atcagcagga
     3361 attcatcgcc acccatgcgg cacagcagat ctggcgcgca gacagcgccg gccatccgct
     3421 cggccacggc ctgcagcacg gcattgccca tggcatggcc ggcgctgtcg ttgatgacct
     3481 tgaagcgatc gacatcgaag atggccagat ggatgcgcgt ggcgcgctcg tccctcagcg
     3541 cggcttccag ccggctcatg aaggcggcgc ggttgagcag gccggtcagc tcgtcggttt
     3601 cggccgcgcg cagcagggcc agctgggcat cgtggcggtc ggtcacatcc tgcagcacca
     3661 ccagcacgct ggagccttgc gggccggcca gggtttcggg cgcgcgcaaa ttggcctcca
     3721 gccagcggcc cgggcgcagc ggcgaggaaa attccagcga cagcacggtt tccggctggg
     3781 ccagcgcctg ggcatagagt tccagcagcg gcgtgcggtt gatcgggctg agcgcgccca
     3841 gatcgcggcc cagcagcgcc cgctcgccaa tgcccagcat cgcccccaca ttgcccacca
     3901 ccttgaggca ggtgccatcg gcggcaaagc ccagcagcag caccgccgcc tcctcggcca
     3961 gcagctgcac cgattgctcg ctttcgcgca gcgcattgag cacgctggcg ctggccgcca
     4021 gcgaggccac taccggcagg atcagcagca cctgcgttgc caggaagaac tggaagaaca
     4081 gcgcccgcgt tggcgcatca tgcgaggcca ggctgatggg gccaaagcca tgcgcggtca
     4141 tgatcgagcc gatcagcgcc agcagcacga tgcccagctg cgtgcccagc cagcccagcc
     4201 ggaaggccac cagcatttcc ggcagcgcca gcacaaacag caccggcagc cgcgaggttt
     4261 cgaacacgcc gatcgcggtg ctggccagca ggccca
//
LOCUS       DASRYE010000268         8788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_44769,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000268 DASRYE010000000
VERSION     DASRYE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44769"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFF98_13795"
     CDS             <1..220
                     /locus_tag="VFF98_13795"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544237.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZU64751.1"
                     /translation="LGAHVLTLASDTGLTDAGLKIRTLRLPDTFQDHDNPDKQYDEAG
                     LTAPHIVQTVLTALRWNEGAIPAEGARA"
     gene            217..672
                     /locus_tag="VFF98_13800"
     CDS             217..672
                     /locus_tag="VFF98_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HZU64752.1"
                     /translation="MSLLIGALAAAALAPMTSAPVTVDVTNVRNDHGMVRVAVCPRDK
                     FLADACPYTASAPAHAGTTRVVVENVPPGDYAAQGFHDENGNDEIDRGLFGIPKEGVG
                     FSRDARIAMSPPKWADAVFSHGGKAQAITFRLRYFMGPGSPQAWAREHR"
     gene            complement(728..1354)
                     /locus_tag="VFF98_13805"
     CDS             complement(728..1354)
                     /locus_tag="VFF98_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64753.1"
                     /translation="MLAARHFWLKTVALAGLALAPVLAAPPALAQQADPAAQAVDQLD
                     NGLIAIMHAGAGAGQAGRAARIAPVVDRVFDIALMTRLSVGPGWTNIAPADQQALVAG
                     FRALTISQYAHNFDSFGGERFAVNGPVETRGADKLVRTSLIVPGHDTELLNYRLRADG
                     GQWKIIDVYYRNSISQLATRRAEFAVVLAKGGAKALISHLNQQAASPR"
     gene            1675..2820
                     /gene="hpnH"
                     /locus_tag="VFF98_13810"
     CDS             1675..2820
                     /gene="hpnH"
                     /locus_tag="VFF98_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZU64754.1"
                     /translation="MSLPLAPALRIARHAITRQIGGGKYPLVLMLEPLLRCNLACKGC
                     GKIDYPDAILNRRLSVEECMAAIEECGAPAVSIAGGEPLLHRDMPKIVEGYIARKKFV
                     ILCTNALLLKKKIGDYKPSSYFTWSIHLDGNKDMHDKAVSQEGVYDVALEAIHLAKSK
                     GFRVQVNCTIFNDANAEGMAKFLDLMASLDVEVTVSPGYAYERAADQEHFLNREKTKT
                     LFRDLFRRGKGGKAWKFTNSPLFLDFLAGNRNYECTPWSMPLRTVFGWQKPCYLVGEG
                     YVDTFAELMEGTDWDQYGVGKYEKCSNCMVHCGFEGTAAADGMRRPWEFIKVGMKGIN
                     TEGPMVPDVDLSQARKAVDVHESQVAEELEKIRIADPEGYRRATRAA"
     gene            complement(2911..3576)
                     /locus_tag="VFF98_13815"
     CDS             complement(2911..3576)
                     /locus_tag="VFF98_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HZU64755.1"
                     /translation="MTGTIIAVTGTRREASVLKGAGLVVLAGGGDAAGLARQIASHLP
                     GAAGIISFGMCGGLDRALKPGQWVIGKRLVGAFPATCDEAWARALAARLPAARLGAIH
                     ADGTLIASADDKLAIANASAALAVDMESHIAARAAAEAGLPFAILRCVSDDAHAELPP
                     AIRLAMRPDGALAVGAMLGSVLRQPGQVPALTRTLAGFAGAFRELAQGAQKVGPGLAF
                     EER"
     gene            complement(3591..5570)
                     /gene="shc"
                     /locus_tag="VFF98_13820"
     CDS             complement(3591..5570)
                     /gene="shc"
                     /locus_tag="VFF98_13820"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZU64756.1"
                     /translation="MLTEPLNTPAPAPAPHTAPPRDAVEASIARASGALLREQQGDGH
                     WVYELEADCTIPSEYILLRHYLAEPDDLELEAKIGRYLRRIQSSTHDGWALYHDGGFD
                     ISATVKAYFALKMIGDDVDAPHMARARAAIHKAGGAEAANVFTRIQLALYGAGPWDVV
                     PSIPPELILAPRWFPVHLLKMSYWARTVVVPLLVLCALKPVARNPRGVKVDELYTGIA
                     RKLTSQAAHVNPLIKAGFEVLDWGLKRFERHWPKTMRQRAINQCKDWVTERLNGVDGL
                     GAIYPAMANSVMMYDVLGYAPDHPHRAIARESVERLLVIKDEEAYCQPCVSPVWDTAL
                     AAHAMLEAGSAEAVEAAHRALDWLKPHQILDTVGDWAEQRADVRPGGWAFQYGNDHYP
                     DLDDTAVVVMAMDRAKDRRGDADAEAIARGAEWTLGLQSHDGGFAAFDADNDKFYLNN
                     LPFADHGALLDPPTADVSARVVSMLAQMGEPLDSPRMKAALSWLEKEQEAEGSWFGRW
                     GVNYIYGTWSVLCALGRAGLASDHPMVARAVEWLKRVQNADGGWGESCDSYTLERQGH
                     EAFRSTASQTAWALLGLMAVGEAQSPEVARGIAWLMANQDGDGLWGQEVYTGGGFPRV
                     FYLRYHGYPRYFPLWALARWRNLTQGNTQRPAWGM"
     gene            complement(5574..6845)
                     /gene="hpnE"
                     /locus_tag="VFF98_13825"
     CDS             complement(5574..6845)
                     /gene="hpnE"
                     /locus_tag="VFF98_13825"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZU64757.1"
                     /translation="MAMARFNRASIAGAGLAGLAAASALAEAGVPVALSDSAARAGGR
                     CRSYFDAALGLTIDNGNHLVLASNPAVAAFRRRVGATTPLAGPEHADFAFIDLASGER
                     WTLRLNDGPLPWWVLVPGRRVPGTQAADYLALSRLLTPRAGETIGERITPAGPLWEKM
                     LHPVLLAVLNTEPAESDATLAANVLKETLAKGGRASAPRIAMPDLAHCFIDPAVGWLA
                     AHGVPLATGRRLKALEYADGRVAALEWADGRQDVAADEAVILAVPPWVAEGLVPGLAV
                     PTDHRSILNAHYAIAAPAGTPAMLGVVGGLAEWIFCHEGRVSVTISGADRLIDAPREE
                     LAARIWADVAAALGLAGAMPAWQIVKEKRATFAATPAQNARRPGQATGFANLFLAGDW
                     VQNGLPSTIEGALRSGENAARLVLGRPPAYG"
     gene            complement(6845..7690)
                     /gene="hpnD"
                     /locus_tag="VFF98_13830"
     CDS             complement(6845..7690)
                     /gene="hpnD"
                     /locus_tag="VFF98_13830"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZU64758.1"
                     /translation="MSATTTMMPADLAAAVSRSSFYAGMRILPRAEREAMFAIYGFCR
                     VVDDIADDQQTPPEARGAALDRWREGVGALYRGGNPGPAAFLAEAVARFDLVEADFIA
                     VIDGMQMDVDEDIRWPDFAKLDLYCDRVASAVGRLSVNIFGMVRAEGVALAHHLGRAL
                     QFTNILRDIDEDAGIGRVYLPAEALALAGISPTTPAALVAEPNLDAAARWLAARAEIH
                     FREAGAILAARPRGHLVAPRLMEAAYSRILARLLAQGWQAPRRRIKVSKLALAFSALR
                     FSLFA"
     gene            complement(7687..8571)
                     /gene="hpnC"
                     /locus_tag="VFF98_13835"
     CDS             complement(7687..8571)
                     /gene="hpnC"
                     /locus_tag="VFF98_13835"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZU64759.1"
                     /translation="MSEQTDLPSVEGLASGKGHHDENFPVASWLVRADARAPILAYYR
                     FARAADDIADNDAAPAAERLRLLAHMRRGLEGPDAAQGAPEAQALGDVCRARDLPLAH
                     AHELLDAFEQDCRVSRYHDWEGLIAYCRLSAMPVGRYVLDVHGEDRAIWPLSDALCAA
                     LQIINHLQDCGKDYRQIDRVYIPLPLLHAVGLDETALGAARARPELLGVIRLLTEKTQ
                     GLLRQSAPFARAIRDVRLATEVAVIQRLAEDLCGLLLTRDPLSEVVHHSKPRAGWLAA
                     GAALGLLARRALPAGGRK"
     gene            complement(8568..>8788)
                     /locus_tag="VFF98_13840"
     CDS             complement(8568..>8788)
                     /locus_tag="VFF98_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HZU64760.1"
                     /translation="GGGGLPQALGIAATGLMLGLYQPVLAFYRRNPLWALALPLVAAF
                     YLGCTLRSAISFYRGTGGMWKGRAQAGK"
BASE COUNT         1458 a         3010 c         3006 g         1314 t
ORIGIN      
        1 tctgggcgcc catgtgctga cgctggcctc cgacaccggc cttaccgacg ccggcctcaa
       61 gatccgcacc ctgcgcctgc ccgacacctt ccaggaccac gacaaccccg acaagcaata
      121 cgacgaagcc ggcctcaccg ccccccacat cgtccaaacc gtcctcaccg ccctcaggtg
      181 gaacgagggg gccatcccgg cggaaggcgc gcgggcatga gcctgctcat cggggccctg
      241 gcggctgcgg ccctggcccc gatgacctct gccccggtga cggtggacgt gaccaatgtg
      301 cgcaatgacc atggcatggt gcgcgtggcc gtgtgcccca gggacaagtt cctggccgat
      361 gcctgcccct ataccgccag cgcgccggcc catgccggca ccacgcgggt ggtggtagaa
      421 aatgtgccgc cgggcgatta tgccgcgcag ggcttccacg acgagaacgg caatgacgag
      481 atcgaccgcg ggcttttcgg tattcccaaa gagggcgtgg gcttttcgcg cgatgcgcgc
      541 attgccatga gcccgcccaa atgggccgat gcggtgttca gccatggcgg caaggcgcag
      601 gccatcacct tccgcctgcg ctatttcatg gggccgggca gcccgcaggc ctgggcgcgc
      661 gagcaccgct gaagcctgcc aaggggcagg cggcccttgg gccagcccac ccccttgaga
      721 gcagccccta gcgcgggctg gcggcctgct ggttgaggtg cgagatcagc gccttggcgc
      781 cgcccttggc cagcaccacg gcaaattcgg cgcggcgggt ggccagctgg ctgatcgagt
      841 tgcgataata gacgtcaatg atcttccact gcccgccatc ggcgcgcagg cgatagttga
      901 gcagctcggt atcatggccc ggcacgatca acgaagtgcg caccagcttg tcggccccgc
      961 gggtttccac agggccgttc acggcaaagc gctcgccgcc gaaactgtcg aaattatggg
     1021 catattgcga gatggtgagg gcgcgaaaac cggccaccag cgcctgctga tcggccggcg
     1081 cgatgttcgt ccagccgggg ccgacggaca ggcgcgtcat cagcgcgatg tcgaacacgc
     1141 gatcgacgac aggggcaatg cgcgccgccc gcccggcctg gccggcgcca gcgcctgcat
     1201 gcatgatggc gatcaggcca ttgtcgagct ggtccaccgc ctgggcggcc ggatcggcct
     1261 gctgggcaag ggcagggggc gctgccagca ccggggccag cgccagcccc gccagagcaa
     1321 ccgttttcag ccagaaatgc cttgccgcca gcatgatcca tcctttctgt ggcgccctgc
     1381 cgaatgttgc gggcgcgagc caatgcctgc cccggcgctc tagagcaggg gggcttgcct
     1441 gacaatgaat gacgcatggg cgaggtcggg ttgatgttgc aatgcggcga caacctgtgc
     1501 cagcgctggc caatgcatgc gccagcgctt gcgggtctgg caaattttac ctatcggagc
     1561 ttgacggcgc gggccaaacc ggctccgtca tcaggccgct ttggtgcccc cgcatgggcg
     1621 ccgggcggcg gcgcgtgtgc gagcggcgtg acaacggctc gtcaggagta agccatgtcc
     1681 cttccccttg ccccggccct gcgcattgcc cgtcatgcca tcacccggca gattggcggc
     1741 ggcaaatatc cgctggtgct gatgctggag ccgctgctgc gctgcaacct tgcctgcaag
     1801 ggctgcggca agatcgatta tcccgatgcc atcctcaacc gccgcctgtc ggtggaagaa
     1861 tgcatggccg cgatcgagga atgcggcgcg ccggcagtct ccatcgccgg gggcgagccg
     1921 ctgttgcacc gtgacatgcc caagatcgtc gagggctata tcgcccgcaa gaagttcgtc
     1981 atcctgtgca ccaacgcgct gctgctgaag aagaagatcg gcgactacaa gccgagctcc
     2041 tatttcacct ggtcgatcca ccttgatggc aacaaggaca tgcacgacaa ggcggtgtcg
     2101 caggaaggcg tctatgacgt ggcgctggaa gcgatccacc tggccaagag caagggcttc
     2161 cgcgtgcagg tgaactgcac catcttcaac gatgccaacg ccgagggcat ggccaagttc
     2221 ctcgacctga tggccagcct cgatgtggaa gtgacggtga gcccgggcta tgcctatgag
     2281 cgcgccgccg atcaggagca cttccttaac cgcgagaaga ccaagaccct gttccgcgac
     2341 ctgttccgcc ggggcaaggg cggcaaggcg tggaagttca ccaactcgcc gctgttcctc
     2401 gatttcctcg ccggtaaccg caattacgaa tgcacgccct ggtcgatgcc gctgcgcacc
     2461 gtgtttggct ggcagaagcc gtgctatctg gtgggcgagg gctatgtcga cacctttgcc
     2521 gagctgatgg aaggcaccga ctgggatcag tatggcgtcg gcaagtatga gaagtgctcg
     2581 aactgcatgg tccattgcgg cttcgagggc acggccgcgg ccgatggcat gcgccgcccg
     2641 tgggaattca tcaaggtggg catgaagggc atcaacaccg aagggccgat ggtgcccgac
     2701 gtggacctgt cgcaggcccg caaggcggtg gatgtgcacg aaagccaggt ggccgaggag
     2761 ctggagaaga tccgcatcgc cgatcctgaa ggctatcgcc gggccacccg cgcggcctga
     2821 tcaggcctga cgccaggaaa aaaggcgctt tgcccgggcg gggcagagcg ccttttttgt
     2881 ggccggcacc ggcggcccga aaagggcggc ttagcgctcc tcgaaagcca gccccgggcc
     2941 cactttctgc gcgccttggg ccagttcgcg aaaagcgccg gcaaagcctg ccagcgtgcg
     3001 cgtcagtgcc ggcacctgcc cgggctggcg cagcacggaa cccagcatgg cccccaccgc
     3061 cagcgcgcca tcggggcgca tggccaggcg aatggccggc ggcagctcgg catgggcatc
     3121 gtccgacacg cagcgcagga tggcaaaggg caggcctgcc tcggccgccg cgcgcgcggc
     3181 aatgtggctt tccatgtcaa ccgcgagggc cgcgctggca ttggcgatgg ccagcttgtc
     3241 gtcggcggag gcaatcagcg tgccatcggc atggatggcg cccaggcgcg ctgccggcag
     3301 gcgggcggcc agggcacgcg cccaggcctc gtcgcaagtg gcgggaaagg cgcccaccag
     3361 ccgcttgccg atcacccact ggcccggctt cagcgcgcga tcgagcccgc cgcacatgcc
     3421 aaagctgata atgccggcag caccgggcag gtggctggcg atctggcggg caaggccggc
     3481 cgcatcgccg ccgccggcca gcaccaccag cccggcccct ttcagcacag aggcttcgcg
     3541 ccgggtgccg gtaacggcga tgatggtgcc ggtcatctca tcctctctgc ttacatgccc
     3601 caggccgggc gctgtgtgtt accttgggtg agattgcgcc agcgcgccag cgcccacagc
     3661 gggaaatagc gcggatagcc atgatagcgc agatagaaca cccgggggaa gccaccacct
     3721 gtgtagactt cctggcccca caggccgtcg ccatcctggt tggccatcag ccaggcgatg
     3781 ccgcgggcga cttctgggct ctgtgcctcg cccaccgcca tcaggccaag cagggcccag
     3841 gcggtctggc tggcggtgga gcggaaggcc tcgtggccct ggcgctccag cgtataggaa
     3901 tcgcagcttt cgccccagcc gccatcggcg ttctgcacgc gcttcagcca ttctaccgcg
     3961 cgcgccacca tcggatgatc gctggccagc cctgcccggc ccagcgcgca cagcaccgac
     4021 caggtgccgt agatatagtt cacgccccag cggccgaacc agctgccctc ggcctcctgc
     4081 tccttctcca gccaggacag cgcggctttc atgcgcgggc tgtccagcgg ctcgcccatc
     4141 tgcgccagca tcgagacaac ccgcgccgag acatcggccg tgggcggatc gagcagggcg
     4201 ccatgatcgg caaagggcag gttgttgagg tagaacttgt cgttatcggc atcgaaggcg
     4261 gcaaaaccgc catcgtggct ttgcaggccc agcgtccatt cggcgccgcg ggcaatcgcc
     4321 tcggcatcgg catcgccgcg ccggtccttc gcgcggtcca tcgccatcac caccacggcg
     4381 gtgtcgtcga gatcggggta atggtcgttg ccatactgga aggcccagcc gcccgggcgc
     4441 acatcggcgc gctgctcggc ccagtcgccc acggtgtcga ggatctggtg cggcttcagc
     4501 cagtcgagcg cgcggtgggc ggcttccacc gcttctgcgc tgccggcttc cagcatggca
     4561 tgggcggcca gcgccgtatc ccacaccggc gagacgcagg gctggcaata ggcctcctcg
     4621 tccttgatga cgagcagccg ttccaccgat tcgcgggcga tggcgcggtg cggatgatcc
     4681 ggcgcatagc ccagcacgtc atacatcatc acgctgttgg ccatggcggg gtagatcgcg
     4741 cccagcccgt ccacgccgtt cagccgttcc gtcacccaat ccttgcactg gttgatggcg
     4801 cgctggcgca tggttttggg ccagtgccgc tcaaaccgct tgaggcccca gtccagcact
     4861 tcgaaaccgg ccttgatcag cgggttcaca tgggcggcct ggctggtcag cttgcgggcg
     4921 atgccggtgt agagttcatc caccttcacg ccgcgcgggt tcctggccac cggcttcagc
     4981 gcgcacagca ccagcagcgg caccaccacc gtgcgcgccc aatagctcat cttgagcaga
     5041 tgcaccggga accagcgcgg cgccaggatc agctcgggcg ggatcgaggg caccacatcc
     5101 cacggcccgg cgccataaag cgccagctgg atgcgggtga agacattggc cgcttctgcc
     5161 ccgccggcct tgtgaatggc cgcgcgcgcg cgcgccatgt ggggcgcatc gacatcatcg
     5221 ccgatcatct tcagcgcgaa ataggccttc accgtggcgg aaatatcgaa gccgccatcg
     5281 tgatagagcg cccagccatc atgggtggac gactggatcc ggcgcagata gcggccgatc
     5341 ttggcttcca gttccagatc gtccggctct gccagatagt ggcgcagcag gatatattcg
     5401 ctggggatcg tgcaatcggc ctccagctcg tacacccagt ggccatcgcc ctgctgttcg
     5461 cgcagcaggg cgccgctggc gcgggcaatg ctggcttcca cggcatcgcg cgggggcgcg
     5521 gtatgggggg caggggcggg ggcaggggta ttcagcggtt cggtcagcat ggtctatcca
     5581 taggcaggcg ggcggcccag caccagccgg gcggcattct cgccgctgcg cagcgcgccc
     5641 tcgatggtgg aaggaaggcc gttctgcacc cagtctccgg ccaggaagag gttggcaaaa
     5701 ccggtggcct gcccggggcg gcgggcgttc tgggccggcg ttgcggcaaa ggtggcgcgc
     5761 ttttccttga cgatctgcca ggccggcatg gcgcctgcaa ggcccagcgc tgctgccaca
     5821 tcggcccaga tgcgggcggc cagttcctcg cgcggggcat cgatcagccg gtctgctccg
     5881 gaaatggtga ccgagacgcg cccctcgtgg cagaagatcc attcggcaag gccgcccacc
     5941 acgcccagca tcgccggcgt gccggcgggc gcggcaatgg cgtaatgggc gttgaggatg
     6001 ctgcgatggt cggtgggcac ggcaaggccg ggcaccagcc cttcggccac ccagggcggc
     6061 acggccagga tcaccgcctc gtctgccgcc acgtcctgcc ggccatcggc ccattccagc
     6121 gcggcaacgc ggccgtctgc atattccagc gccttcagcc ggcggccggt ggccagcggc
     6181 acgccatgcg cggccagcca gcccaccgcc ggatcgatga agcaatgggc cagatcgggc
     6241 atggcgatgc gcggcgcgct ggcccgcccg cctttggcca gcgtttcctt cagcacattg
     6301 gcggcaaggg tggcatcgct ttcggcgggc tctgtgttga gcacggcgag cagcaccggg
     6361 tgcagcatct tctcccacaa gggcccggcc ggcgtgatgc gctcgccgat cgtctcgccc
     6421 gcgcgggggg tgagcaggcg cgagagggcg agataatccg ccgcttgcgt gcccggcacc
     6481 cggcggccgg gcaccagcac ccaccagggc agcgggccat cgttgagccg cagcgtccag
     6541 cgctcgcccg aggcaaggtc gatgaaggca aaatcggcgt gttcggggcc tgccagcggg
     6601 gtggtggcgc ccacccggcg gcggaaggcg gccacggccg ggttgctggc cagcaccaga
     6661 tggttgccat tgtcgatagt gaggcccagc gctgcgtcga aatagctgcg gcagcgcccg
     6721 ccggcgcgcg ccgcgctgtc cgacagggcc accggcacgc ccgcctcggc cagcgcgctg
     6781 gcggcggcaa ggccggcaag gccggcgccg gcaatgcttg cccggttgaa acgcgccatc
     6841 gccatcacgc gaacaggctg aagcgcaggg ccgagaaggc cagcgccagc ttcgacacct
     6901 tgatccgccg gcgcggcgcc tgccagcctt gcgccagcag ccgggcaaga atgcgcgaat
     6961 aggccgcttc catcagccgc ggcgccacca gatggccgcg cgggcgggcg gcgagaatgg
     7021 cgccggcctc gcggaaatgg atttcggcgc gcgcggccag ccagcgcgcg gcggcatcga
     7081 ggttgggctc tgccaccagc gctgcggggg tggtggggct gatgccggcc agcgccagcg
     7141 cttctgccgg cagatagacg cggccgatgc cggcatcctc gtcgatatcg cgcaggatgt
     7201 tggtgaactg cagcgcccgg cccagatgat gggccagcgc caccccttct gcccgcacca
     7261 tgccgaagat gttgaccgac aggcggccaa ccgcgctcgc cacccggtcg caatagagat
     7321 cgagcttggc gaaatcgggc cagcggatgt cttcgtccac atccatctgc atgccatcga
     7381 tcaccgcgat gaaatccgcc tcgaccagat cgaaccgcgc gaccgcttcg gcaaggaagg
     7441 ccgccggccc gggattgccg ccgcgataga gcgcgcccac cccctcgcgc cagcgatcga
     7501 gcgcggcgcc gcgggcctcg ggcggggtct gctggtcgtc ggcgatatcg tccaccacgc
     7561 ggcagaagcc atagatggcg aacatcgcct cgcgctcggc cctgggcagg atgcgcatgc
     7621 cggcatagaa gctggagcgc gagacggccg ctgccagatc ggcgggcatc atggtggtgg
     7681 tcgcgctcat ttgcggcctc ctgcgggcag ggcgcggcgg gccagcaggc ccagcgcggc
     7741 gcccgccgcc agccagccgg cgcgcggttt ggaatggtgc accacctcgc tcagcggatc
     7801 acgggtgagc agcaggccgc acagatcctc ggccagccgc tggatcaccg ccacttcggt
     7861 ggccaggcgc acatcgcgga tggcgcgggc aaagggcgcg ctctggcgca gcaggccttg
     7921 cgtcttttcc gtcagcaggc ggatcacgcc cagaagctcg gggcgggcgc gggcagcgcc
     7981 cagcgccgtc tcgtccagcc ccacggcatg gagcagcggc agcgggatat agacccggtc
     8041 gatctggcgg taatccttgc cgcaatcctg caggtggttg atgatctgca aggccgcgca
     8101 caaggcatcg gagagcggcc agatcgcccg gtcctcgccg tgcacatcca gcacatagcg
     8161 gcccaccggc atggccgaga ggcggcaata ggcgatcagc ccttcccaat cgtgatagcg
     8221 gctgacccgg caatcctgct cgaaggcatc gagcagttca tgggcatggg ccaagggcag
     8281 gtcgcgcgcg cggcacacat cgcccagcgc ctgcgcctcg ggggcgccct gcgcggcatc
     8341 gggcccttca aggccgcggc gcatgtgggc cagaaggcgc agccgctcgg cggcgggggc
     8401 ggcgtcattg tcggcaatgt cgtcggcggc gcgggcaaag cggtagtagg ccaggatggg
     8461 cgcgcgggca tcggcgcgca ccagccaact ggccaccggg aagttctcgt cgtgatggcc
     8521 cttgcccgag gcaagcccct ccacgctcgg cagatcggtc tgttcgctca tttgccggcc
     8581 tgcgcccgcc ctttccacat gccgcccgtg ccgcgataga agctgatcgc cgagcggagc
     8641 gtgcagccaa gatagaaagc ggccaccagc ggcaatgcca gcgcccacag cggattgcgc
     8701 cgatagaagg ccagcaccgg ctggtaaagc cccagcatca gcccggtggc ggcaatgccc
     8761 agcgcctgcg gcaggccgcc gccgcccg
//
LOCUS       DASRYE010000269         6217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_30688,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000269 DASRYE010000000
VERSION     DASRYE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30688"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFF98_13845"
     CDS             <1..375
                     /locus_tag="VFF98_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZU64761.1"
                     /translation="AARARGLAVVVAGDAALARRWRADGAYGAAARLAGGGALLRLIT
                     AHSLAEIAAAHRARASAILLSPAFATRSHPGAPALGPVRWRLLAARCLVPVIALGGLT
                     RHSARRLGARACHGWAAIDGLS"
     gene            488..2920
                     /locus_tag="VFF98_13850"
     CDS             488..2920
                     /locus_tag="VFF98_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZU64762.1"
                     /translation="MATRYTPGRAGGRAAARGRAPWRLALRRSLKRSAELLGAGALAL
                     AMLFLALALLSYTQTDPSPSTAAGGPVQNWMGSLGAIVAERALFLFGPVSALLLPLMF
                     VFARQLWRAAEAAIARPADEDEDEDEEEAPARILWWQPLGMLVLAMFGLCTALSLVFT
                     QPGWQLPASMGGIAGLLGAAALRGLAHFLPQAWQGWVMVAGGLTALALGAVLTTRVFA
                     IDWGGLFTIDLGALLHRRRAPAKPEALPEVPFALDDEEDEDDSAPAQPRTGEDAPAPR
                     PRKATKISEPTGSARAADTGTLPRKRDLFDAYELPQLELLTDAPPNTGAKVDKLALER
                     NARLLETVLDDFNVKGEITGVRTGPVVTMYELEPAPGVKASRVIGLADDIARNMSAIS
                     ARVSPVPGRTVMAIELPNADRQMVSFKDMVASESFHAAKGLLPIILGKDIAGEPSVAD
                     LAAMPHLLVAGTTGSGKSVGLNCILLSLLYRLTPQQCRLILVDPKVLELKSYDAIPHL
                     LSPVVTEPAKAVRALKWAVEEMERRYRQMSELSVRNITGFNEKVRNAQAKGKGLGRRV
                     SIGFDPETGEELFEDKTLDYQVLPLIVVIVDELADLMVTVGKEIEVLIQRLSQKSRAA
                     GIHLIMATQRPSVDVITGVIKANLPTRISFAVTSRIDSRTILGEQGAEQLLGKGDMLF
                     KPSSGPILRVHGPFVSDEEVERVADHWRAQGSPEYIDSVTEEPEEGSIGFDDLEATAS
                     DNPEERKYRQACQIVFESQKASTSWLQRQMTVGYNTAAKWIERMEEDGLVGPANHVGR
                     REIYRDKDGNPL"
     gene            3161..3646
                     /locus_tag="VFF98_13855"
     CDS             3161..3646
                     /locus_tag="VFF98_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HZU64763.1"
                     /translation="MFAASLVAASVGGASAAAAHTQCAPYAREVSGIDLHGAAASWWG
                     EAAGNYGRGNAPKMGAVLAFRATHAMRSGHVAVVEKVVDARHVLLNHANWSGRGAIEH
                     GAMAEDVSANGDWTEVRVWYAPIHALGLRASPTFGFIYKDAPKSAELALADDTAPKSH
                     S"
     gene            complement(3662..4087)
                     /locus_tag="VFF98_13860"
     CDS             complement(3662..4087)
                     /locus_tag="VFF98_13860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZU64764.1"
                     /translation="MSQPFTLIFTAGPEHIDVMGHVNNAVWVQWMETIATAHWQAQAL
                     PEHAARFVWVVTRHEIDYRGNIRAGEQVLARTFIPAAPQGARFDRRVDFCPLGADGAA
                     GKVIVSARTTWAMIEKESGRLCRIPPEVAAPFMLADQAG"
     gene            complement(4084..6201)
                     /locus_tag="VFF98_13865"
     CDS             complement(4084..6201)
                     /locus_tag="VFF98_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZU64765.1"
                     /translation="MQAMANSTPPAAQPPLAPKRPTSFTRHGITIADDYAWLRDPAYP
                     EVTEPDILAHLEAENAWFEARMAPRQPLVDELFAEMRGRIKEADASVPQKDGNWLYWI
                     SYEEGAEYKKWWRRPVGAPADGSADELILDEVALAEGHDYFRLGAISVSTDGRLLAWS
                     VDDNGSERFTARVKVIATGERLPDEIPGTLSGLVWVAGDRGLVYSLANENWRTDNARL
                     HWLGRPLAEDVELYHEDDEGFRVGAALSANEEWLIVSTSDHETSEVRLIPTADPLAEP
                     LLVRPRQEGVEYDVDLREETLFIHANDTHENFRLATAPLAEPAAWTSLVEGSDDFYLT
                     GFDLFRDFYVVEGRVRGLDRIEVRYYDAPGRVEPITFPEASYEAALADNPEWAVDRLR
                     VSYESMVSPASVYDYHVAEQRLEWLKTQEIPSGYDASLYETQRLEIPARDGTLVPVSI
                     VWRRDRAPAGPLHLYGYGAYGIAIGPGFSTARLSLVDRGFAYAIAHIRGGDDMGRAWY
                     KAGKREARTNTFHDFVDVARGLVAAGFTAAGRISISGGSAGGELMGAVINSDPALWGA
                     VVAHVPFVDVLNTMLDETLPLTPGEWPEWGNPIEDKAAFELIHSYSPYDQVAAQPYPP
                     LMVTAGLNDPRVTYWEPAKWVARLRALKTDGNELIFKTNMGAGHGGKSGRFESLTETA
                     EEFAFILWQLGIAGLAGAAGVAG"
BASE COUNT          984 a         2151 c         2094 g          988 t
ORIGIN      
        1 gcggcaaggg cgcgcgggct ggcggtggtg gtggcgggcg atgcggcgct ggcccggcgc
       61 tggcgggccg atggcgccta tggcgcggcg gcgcggctgg cgggcggcgg tgccctgctg
      121 cggctgatca ccgcccacag cctggccgag atcgccgccg cccaccgcgc gcgcgccagc
      181 gccatcctgc tctcgcccgc ctttgccacc cgcagccacc ccggcgcgcc ggcgctgggc
      241 ccggtgcgct ggcgcctgct ggcggcgcgc tgcctggtgc cggtgatcgc gctgggcggc
      301 ctcacccgcc acagcgcccg ccggctgggc gcgcgcgcct gccacggctg ggcggcgatc
      361 gacgggttga gctgatcccc ctttcccaca tacccttgcg gcgccttgct tgacggccca
      421 cagcgcccgg ccgcataccg ccggcttggc acagggattc tggcaaggga cacaggggca
      481 ggtcggcatg gcaacgcgct acactccggg gcgagcgggc ggacgggcgg cggcacgcgg
      541 gcgggcgccc tggcggctgg cgctgcgccg cagcctgaag cgcagcgccg agctgctggg
      601 cgccggcgcg ctggcgctgg ccatgctgtt cctggccctg gcgctcctca gctacaccca
      661 gaccgacccc tctccctcca ccgccgcggg cgggccggtg cagaactgga tgggcagcct
      721 tggcgccatc gtggcggaac gggcgctgtt cctgttcggc ccggtgtcgg cgctgctgct
      781 gccgctgatg ttcgtgtttg cccgccagct gtggcgcgcg gccgaagccg ccatcgcccg
      841 cccggccgat gaggacgagg acgaggacga ggaggaggcg ccggcccgca tcctgtggtg
      901 gcagccgctg ggcatgctgg tgctggcgat gtttggcctg tgcacggcgc tgtcactggt
      961 gttcacccag ccgggctggc agctgccggc ctcgatgggg gggattgccg gtctgctggg
     1021 ggcagcggcc ctgcgcggcc tcgcccattt cctgccgcag gcctggcagg gctgggtgat
     1081 ggtggccggc gggctgaccg cgctggcgct gggcgcggtg ctgaccacgc gggtgttcgc
     1141 catcgattgg ggcgggctgt tcaccatcga tctgggcgcg ctgctgcacc gccgccgggc
     1201 gccggccaag cctgaggccc tgcccgaagt gcccttcgcg ctggacgatg aggaagacga
     1261 ggacgacagt gcccccgcgc aaccgcgcac cggcgaggac gcccccgccc cccgcccgcg
     1321 caaggccacc aaaatcagcg agcccaccgg cagcgcccgc gccgccgaca ccggcaccct
     1381 gccgaggaag cgcgacctgt tcgacgccta tgaactgccc cagctggagc tgctgaccga
     1441 cgccccgccc aacaccggcg ccaaggtgga caagctggcg ctggagcgca acgcgcgcct
     1501 gctggaaacc gtgctcgacg atttcaacgt gaagggcgag atcaccggcg tgcgcaccgg
     1561 ccctgtcgtc accatgtacg agctggagcc ggcgccgggc gtcaaggcca gccgggtgat
     1621 cggcctggcc gacgacatcg cccgcaacat gagcgccatt tccgcccgcg tgagcccggt
     1681 gcccggccgc acggtgatgg ccatcgaact gcccaatgcc gaccgccaga tggtctcctt
     1741 caaggatatg gtggccagcg aaagcttcca tgccgccaag ggcctgctgc ccatcatcct
     1801 gggcaaggat atcgccggcg agccatcggt ggcggatctg gccgccatgc cccatttgct
     1861 ggtggcgggc accacgggtt cgggcaagtc ggtggggctg aactgcatcc tgctctcgct
     1921 gctctatcgc ctcacgcccc agcagtgccg gctgatcctc gtcgatccca aggtgctgga
     1981 gctgaagagc tacgatgcca tccctcacct cctcagcccg gtggtgacag agcccgccaa
     2041 ggcggtgcgc gcgctcaaat gggcggtgga ggagatggag cgccgctatc gccagatgag
     2101 cgagctttcc gtgcgcaaca tcaccggctt caacgagaag gtgcgcaacg cccaggccaa
     2161 gggcaagggg ctgggccggc gcgtgtcgat cggcttcgat ccggaaaccg gcgaggaact
     2221 gttcgaggac aagacgctgg attaccaggt gctgccgctg atcgtcgtca tcgtcgacga
     2281 gctggccgac ctgatggtga cggtgggcaa ggagatcgag gtgctgatcc agcgcctcag
     2341 ccagaagagc cgcgcggccg gcatccacct catcatggcc acgcagcgcc cctcggtgga
     2401 tgtgatcacc ggcgtcatca aggccaacct gcccacgcgc atcagctttg ccgtcaccag
     2461 ccggatcgat tcgcgcacca ttctgggcga gcagggcgcc gagcagttgc tgggcaaggg
     2521 cgacatgctg ttcaagcctt cttccggccc gatcctgcgc gtgcatggcc cctttgtctc
     2581 cgacgaggaa gtggagcgcg tggccgatca ctggcgcgcc cagggcagcc cggaatatat
     2641 cgattcggtg accgaggagc ccgaggaagg cagcatcggc ttcgacgatc tggaagccac
     2701 cgccagcgac aaccccgagg aacgcaaata ccgccaggcc tgccagatcg tcttcgagag
     2761 ccagaaggcc tccaccagct ggctgcagcg gcagatgacc gtgggctaca acaccgccgc
     2821 caaatggatc gagcggatgg aggaagacgg gctggtgggc ccggccaacc atgtcggccg
     2881 gcgcgagatc tatcgcgaca aggatggcaa tccgctctga ccccacccgg gatgtgcctt
     2941 aagggagatt gcggattgcc ggcacactcg gttcatcccc atgctgcagc ggcgaaagga
     3001 gacgaaccgt tgccccggca cggccacttg caggctgcct gtcttccgaa agaaattttt
     3061 cccctgtagc actagggcca caccacggac gggtggcgtt aacaggggcg tttcggtgct
     3121 gggcaaattc ggttcggttt ttggtatcaa ggccaagcac ttgtttgccg cttcgctggt
     3181 tgccgcctcg gttggcgggg cttcggccgc cgccgcccac acccagtgcg cgccctatgc
     3241 gcgtgaagtt tccggcatcg atctgcatgg cgccgccgct tcctggtggg gcgaggctgc
     3301 cggcaattat ggccgcggca atgcccccaa gatgggcgcc gtgctggcct tccgcgccac
     3361 ccatgccatg cgttcgggcc atgtcgccgt ggtggaaaag gtggtcgacg cgcgccacgt
     3421 gctgctgaac cacgccaact ggtcgggccg cggcgcgatc gagcatggcg ccatggccga
     3481 agacgtttcc gccaatggcg actggaccga ggtgcgcgtg tggtatgccc cgatccatgc
     3541 gctgggcctg cgcgcctcgc ccactttcgg cttcatctac aaggatgcgc ccaagagcgc
     3601 cgaactggcc ctggccgacg acaccgcccc caagagccat tcgtaaccgg ccctggcgcc
     3661 ctcagccggc ctgatcggcc agcatgaagg gggcggccac ttcgggcgga atgcggcaca
     3721 atcggccgct ctccttctcg atcatcgccc aggtcgtccg cgccgagacg atcaccttgc
     3781 ccgccgcgcc gtccgcgccc agcgggcaga aatccacccg ccggtcgaaa cgcgcgccct
     3841 ggggcgcggc agggatgaag gtgcgcgcca gcacctgttc gcccgcgcgg atattgcccc
     3901 gataatcaat ctcgtgccgg gtgacgaccc agacgaaacg cgccgcatgt tccggcaggg
     3961 cctgcgcctg ccagtgggcc gtggcgatcg tttccatcca ctgcacccac acggcattgt
     4021 tcacatggcc catcacatcg atatgttcag ggccagcggt gaagatcagg gtgaagggct
     4081 ggctcacccg gcaacgcccg cagcacccgc aaggcccgca atgcccagct gccagaggat
     4141 gaaggcgaat tcctccgcgg tttccgtaag gctttcaaag cggcccgatt tgccgccatg
     4201 cccggcgccc atattggtct tgaagatcag ctcgttgcca tccgtcttca gcgcgcgcag
     4261 gcgggccacc catttcgccg gttcccaata ggtcacgcgc gggtcgttca ggcccgccgt
     4321 caccatcagc ggcggatagg gctgcgctgc cacctgatca tagggcgaat aggagtggat
     4381 cagctcgaaa gcggccttgt cctcgatggg gttgccccat tcgggccatt cgcccggcgt
     4441 caacggcagg gtttcatcca gcatggtgtt cagcacatcg acgaaaggca catgcgccac
     4501 caccgcgccc cacagcgccg gatcggaatt gatcaccgcg cccatcagct cgccgccggc
     4561 agagccgccc gagatgctga tgcgcccggc cgcggtgaag ccggccgcca ccagccccct
     4621 ggccacatcg acaaaatcgt ggaacgtgtt ggtgcgcgcc tcgcgcttgc cggccttgta
     4681 ccaggcgcgg cccatatcgt cgccgccgcg gatatgggcg atggcatagg caaagccgcg
     4741 atcgaccagg ctgagccgcg cggtggaaaa gcccgggcca atggcaatgc cataggcgcc
     4801 atagccatag agatgcagcg gcccggccgg cgccctgtcg cggcgccaga cgatggacac
     4861 cggcaccagc gtaccatcgc gcgcggggat ctccagccgc tgcgtttcat acaggctggc
     4921 atcatagccg ctggggattt cctgcgtctt cagccattcc agccgctgtt cggccacgtg
     4981 ataatcatag acgctggccg ggctgaccat gctttcatag ctcacccgca gccggtccac
     5041 cgcccattcc gggttgtcgg caagcgctgc ctcatagctg gcctcgggga aggtgatcgg
     5101 ctccacccgc ccgggcgcgt cgtagtagcg cacctcgatc cggtcgagcc cgcgtacccg
     5161 gccttccacc acgtagaaat cgcggaagag atcaaagccg gtcaggtaga aatcatcgct
     5221 gccttccacc aggctggtcc aggcggcagg ctctgccagc ggcgctgtgg ccaggcgaaa
     5281 gttctcatgc gtgtcgttgg cgtggatgaa cagcgtctcc tcgcgcaggt ccacgtcata
     5341 ttccacgcct tcctggcggg ggcgcaccag cagcggctct gccagcggat cggcggtggg
     5401 gataaggcgc acctcggagg tttcatgatc gctggtggaa acgatcagcc attcctcatt
     5461 ggccgacagc gccgcgccca cgcgaaagcc ctcgtcgtcc tcgtggtaga gctcgacatc
     5521 ctcggccagc gggcggccca gccagtgcag gcgggcattg tcggtgcgcc agttctcgtt
     5581 ggccagcgaa tagaccagcc ccctgtcgcc cgccacccac accaggcccg acagcgtgcc
     5641 ggggatctcg tcgggcaggc gttcgccggt ggcgatcacc ttcacccggg cggtgaagcg
     5701 ttcagagccg ttgtcgtcca cgctccaggc cagcaggcgc ccgtcggtcg agaccgaaat
     5761 cgcccccagg cggaaatagt catgcccctc ggccagcgcc acctcgtcga ggatcagctc
     5821 atcggcgctg ccatcggcgg gggcgcctac cgggcggcgc caccatttct tgtattcggc
     5881 gccttcctca tagctgatcc agtagagcca gttgccatcc ttctgcggca ccgaggcatc
     5941 ggcttccttg atgcgcccgc gcatctcggc aaacagttca tcaaccagcg gctggcgcgg
     6001 ggccatgcgc gcctcgaacc aggcgttctc ggcttcgaga tgggcgagga tgtcgggctc
     6061 tgtcacttcc ggataggccg gatcgcgcag ccaggcgtaa tcgtcggcaa tggtgatgcc
     6121 gtggcgggtg aagctggtgg ggcgcttggg cgccagcggc ggctgggctg cggggggagt
     6181 ggaattggcc atggcttgca tctatgttgc cagcccg
//
LOCUS       DASRYE010000270         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_122802,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000270 DASRYE010000000
VERSION     DASRYE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122802"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1269)
                     /locus_tag="VFF98_13870"
     CDS             complement(46..1269)
                     /locus_tag="VFF98_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64766.1"
                     /translation="MSDKSFWQSGHRPTLIMAFLYFDIAFMVWNLLGPLAPIFSKVLA
                     LSPAQKGFVVAVPTLAGAVLRLVNGFLADRIGQKTTGIISQVIVIAGLLAGWQLGIHT
                     MGGLIAMGLVLGFAGASFSVALPLAGRWYPPQHQGKAMGIAGMGNSGTVLAALFAPLL
                     AKAFGWTNVLALATIPAAIVLVAFVALAKDSPNQPEAKPLGAYVAGYFRMLAVGDAWW
                     FILFYGVTFGGFVGLSGSLPSWYTAELAMTPVQAGYATAACVFAGSLVRPMGGALADR
                     IGGIKTLSGVYAIAAVLLVTISQMPANFAIMFPLFFSVMAVLGVGNGAVFQLVPQRFR
                     HEIGVMTGLVGFGGGVGGFYLASSLGLAKQWTGSMGPGFLVFAVLAVLALAGLTSVKK
                     RWRTTWGAAIAGVRI"
     gene            1364..>2168
                     /locus_tag="VFF98_13875"
     CDS             1364..>2168
                     /locus_tag="VFF98_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZU64767.1"
                     /translation="MLTNGRLWVSVGVTSEQGPRADNQDFAAAYLGSETQRARQGLVA
                     ALADGVSGAKGGRMAAELAVSSLIDGYYAQGDTIGPAAAVERVMRAYNRWLAAMGRSE
                     AMAHACSTFTAIVVRGRRAHVLHVGDSRAWLLRDGHLTQITADHTRSHPDQRHILLRA
                     LGLEEQLRLDHHEIALAEHDRLLLTSDGVHGPLSARRLGRLLGRRQSAEADAAALVEA
                     ALAAGGQDNATALVLDIVSLPSASHDAIAGDMARLPILPPPQVGDSVDGF"
BASE COUNT          388 a          772 c          722 g          286 t
ORIGIN      
        1 gctggcaggc caatacccgg gccgaatgga gccgcatctg aagcctcaga tgcgcacgcc
       61 ggcgatggcc gcgccccagg tggtgcgcca gcgcttcttc acgctggtga ggcccgccag
      121 cgccagcacc gccagcaccg caaagacgag gaagcccggc cccatgctgc ccgtccactg
      181 cttggccagg cccagcgagg aggcgaggta gaagccgccg acgccgccgc cgaagccgac
      241 caggcccgtc atcacgccga tctcgtggcg gaagcgctgc ggcaccagct ggaacaccgc
      301 gccattgccc acgcccagca ccgccatcac gctgaagaac agcgggaaca tgatggcgaa
      361 attggccggc atctggctga tcgtcaccag cagcacggcg gcaatggcat aaacgcccga
      421 cagggtcttg atgccgccga tgcgatcggc cagcgcgccg cccatcggcc gcaccagcga
      481 accggcaaac acgcaggccg ccgtggcata gcccgcctgc accggggtca tcgccagttc
      541 agcggtatac cagctgggca gcgagccgga gaggccgaca aagccgccaa aggtgacgcc
      601 atagaacagg atgaaccacc aggcatcgcc caccgccagc atgcggaaat agccggcgac
      661 ataagcgccc agcggcttgg cctcgggctg gttggggctg tccttggcca gcgccacaaa
      721 ggccaccagc acgatcgctg ccggaatggt cgccagcgcc agcacattcg tccagccaaa
      781 ggccttggcc agcagcggcg cgaacagggc ggccagcacc gtgccggaat tgcccatgcc
      841 ggcaatgccc atcgccttgc cctggtgctg cggcggatac cagcgccccg ccagcggcag
      901 cgccaccgag aaggacgcgc cggcaaagcc cagcaccagc cccatggcga tcagcccgcc
      961 catcgtgtga atgcccagct gccagcccgc cagcaggccg gcaataacga tcacctggct
     1021 gatgatgccg gtggtcttct ggccgatgcg atcggcgagg aagccgttga ccaggcgcag
     1081 caccgcgccc gccagcgtgg gcaccgccac gacaaagccc ttctgcgccg ggctcaatgc
     1141 cagcaccttg gaaaagatcg gcgccagcgg ccccagcagg ttccacacca tgaaggcgat
     1201 gtcgaaatag aggaaggcca tgatcagtgt cggcctgtgg ccggattgcc agaaggattt
     1261 gtcagacatt gcgattactc ccgtgcaggt ggattttaac agcttgactg gcccgcccgc
     1321 cagcgctccc cttcgcgtac gtgtgcgaag gggatggttg ggcatgctga cgaacgggcg
     1381 cttgtgggta tcggtggggg tgaccagcga gcagggcccg cgggccgaca accaggattt
     1441 tgccgccgcc tatctgggca gcgaaaccca gcgcgcccgg caggggctgg tggccgcgct
     1501 ggccgatggg gtgagcggcg ccaagggcgg gcgcatggcc gccgaactgg ccgtctccag
     1561 cctgatcgac ggctattacg cgcagggcga cacgattggc ccggccgccg ctgtggaacg
     1621 ggtgatgcgc gcctacaacc gctggctggc cgccatgggc cgcagcgagg cgatggccca
     1681 tgcctgctcc accttcaccg ccattgtcgt gcgcgggcgg cgggcccatg tgctgcatgt
     1741 gggcgacagc cgcgcctggc tgctgcgcga tggccacctc acccagatca ccgccgacca
     1801 cacccgcagc caccccgatc agcgccacat cctgctgcgc gcgctggggc tggaagagca
     1861 gctgcggctg gatcaccacg agattgccct ggccgagcat gaccggctgc tgctcaccag
     1921 cgatggtgtg catggccccc tttcggcccg gcgccttggc cggctgctgg ggcggcgcca
     1981 gtcggccgag gcggatgccg cagcgctggt ggaagccgcc cttgccgccg gcggacagga
     2041 caacgccacc gccctggtgc tcgatatcgt cagcctgccc tcggccagcc acgatgccat
     2101 cgccggcgac atggcccgcc tgccgatcct gccgccgccg caggtgggcg acagtgtcga
     2161 tggcttcc
//
LOCUS       DASRYE010000271         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_245828,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000271 DASRYE010000000
VERSION     DASRYE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245828"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFF98_13880"
     CDS             complement(<1..133)
                     /locus_tag="VFF98_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HZU64768.1"
                     /translation="MEETPALLALADRMLWVVALAGAPVLFTCLAVGLVIGILQAATS
                     "
     gene            complement(133..906)
                     /gene="fliP"
                     /locus_tag="VFF98_13885"
     CDS             complement(133..906)
                     /gene="fliP"
                     /locus_tag="VFF98_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012324.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZU64769.1"
                     /translation="MKRLLAAALLLLAPGQALAQAAAGGGAAPSALSGALDRAFSQMN
                     GGPGAGLSLSLQLLLIMGLLTILPSLILMMTSFTRILVVLSILRQALGLQQSPPNQVL
                     IGLALFLSLFVMAPTLDHVNQNAIAPYAAGQINAQDAIGRAGTEFHAFMIRQTRKTDL
                     AMFAGMAKAPPFARPEDVPFSILLPAFVTSELKTAFQIGFMLYLPFLVIDLVVSSVLM
                     SLGMMMMSPTVVSLPFKLLLFVMVDGWSLLMGSLAASFG"
     gene            complement(903..1181)
                     /locus_tag="VFF98_13890"
     CDS             complement(903..1181)
                     /locus_tag="VFF98_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZU64770.1"
                     /translation="MLWYVLKLVVLLPLIGGLIWASLRFSQRLQARLGAPAGGHAVRL
                     VESLILSPTQKLIVIEFHGREILVAASRQGFTRLAEAPARPAPQDRLP"
     gene            complement(1181..1480)
                     /gene="fliN"
                     /locus_tag="VFF98_13895"
     CDS             complement(1181..1480)
                     /gene="fliN"
                     /locus_tag="VFF98_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZU64771.1"
                     /translation="MSFPSPPPRGFDFLKDVDVRLSVELGRAQMKLKDVLSLGEESVV
                     MLDRLTDELLDIMVNGKVIAKGEIVAHGGRFGLRIVEMAGSEGTPVESPAPRGEI"
     gene            complement(1477..2448)
                     /locus_tag="VFF98_13900"
     CDS             complement(1477..2448)
                     /locus_tag="VFF98_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZU64772.1"
                     /translation="MKPERAFIAERTLARHCPELLRQGPTRADLLPLLERMGGRLERK
                     LADAFAPMLFSQQGAKPPLLHCARPRETDAGALMQSIAPLAANSLFAVGAGAAPLLVS
                     IEAEPVLRMVDRAFGGKGEAPAPMPKTFPMAAELMIARLEKLLAEQVGLAIAALGPAG
                     AGRTAGAPAITPLRRDGSLAMLAPFAASLPLIAMTIEVDDGSVLPWLVTIALPEPTLF
                     ALFGCAAPGSDAPARRQRRRPMASPTATPFADMPVEVVATLVDMQVPFATIAGLAPGQ
                     ILPVAVARAVPLSVADVAIAHGTVGALDERVALQLTRAFDDSRSPAS"
     gene            complement(2461..3048)
                     /locus_tag="VFF98_13905"
     CDS             complement(2461..3048)
                     /locus_tag="VFF98_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZU64773.1"
                     /translation="MSKDKTESEAPPKKGKGPLMLIIVAVAMLAVGGGGAFAMVKLGV
                     LGGGEKVHEDNSPKLIHKGDTDPFAPKAEGGKEGATPEVEGDGGSPFKTSYYNFSEDF
                     TSNLKNSSALIQVSLACSTHRDGRVLMWLKKHELAVRSAMLEVLADTPEDDVNTIQGK
                     ERLQKRLADAINHVLTQEEGFGGVDNVYFKTFLVQ"
     gene            complement(3083..>3804)
                     /locus_tag="VFF98_13910"
     CDS             complement(3083..>3804)
                     /locus_tag="VFF98_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64774.1"
                     /translation="NAAPGTTHAATPGASQPAVATAAPAPGLAVNQAAALAASQAAAS
                     PAAAGPGASVPVSSVTAATGQDMAALVDRLVEARAAARAGSPAVVQTAISHAAFGQVA
                     LRFQTDATGTLSVAMSSSDPAFAASAQAALAAAPHAAGALTSVHAAAISAAITPAAAG
                     SATGDAPSDNTGQNGGQNSGNTQNGPGAGHNAATGQDSARQGQGAQRGAPAPLASAQP
                     AATATDNAADAALSTAQGIFA"
BASE COUNT          603 a         1246 c         1393 g          562 t
ORIGIN      
        1 ccgaggtggc cgcctgcaaa atgccgatca ccagccccac cgcaaggcag gtgaacagca
       61 ccggcgcgcc cgccagcgcc accacccaca gcatgcgatc ggccagggcc agcagggcgg
      121 gggtttcctc catcagccga aactggccgc caggctgccc atcagcagcg accagccatc
      181 caccatgaca aacagcaaca gcttgaacgg cagggaaacc accgtcgggc tcatcatcat
      241 catgcccagg ctcatcaaca ccgaggagac gaccagatcg atcaccagaa acggcagata
      301 gagcatgaag ccgatctgga aggcggtttt cagctcgctc gtcacaaagg cgggcagcag
      361 gatcgagaag ggcacatctt ccggccgggc aaagggcggc gccttggcca tgccggcaaa
      421 catggcgaga tcggtcttgc gggtctggcg gatcatgaag gcgtgaaatt cggtgccggc
      481 cctgccgatg gcatcctgcg cgttgatctg cccggccgca tagggggcga tggcgttctg
      541 gttcacatga tcgagcgtgg gcgccatgac gaacagcgac agaaacagcg ccaggccgat
      601 cagcacctgg ttgggcgggc tttgctgcag ccccagcgcc tgccgcagga tcgacagcac
      661 gacgagaatg cgcgtgaagc tggtcatcat caggatgagg ctgggcagga tcgtcagcag
      721 ccccatgatg aggagcagct ggagcgacag cgagaggccg gcgcccggcc cgccgttcat
      781 ctgcgaaaag gcgcgatcaa gcgcgccgga aagcgcggaa ggcgccgcgc cgccacctgc
      841 tgccgcctgc gccagcgcct ggccgggggc cagcagcagg agggcggcgg ccagcagccg
      901 cttcatggca gcctgtcctg cggggcgggg cgggccgggg cttcggccag gcgggtgaac
      961 ccctggcggc tggcggcgac caggatctcg cggccatgaa attcgatgac gatcagcttt
     1021 tgcgtgggcg agaggatcag gctttccacc agccgcaccg catgcccccc cgccggcgcc
     1081 cccagccttg cctgcagcct ttgcgaaaag cgcagcgagg cccagatcag cccgccgatc
     1141 agcggcagca gcaccaccag cttgaggaca taccagagca tcagatctct ccgcgcgggg
     1201 cggggctttc gacaggggtg ccctcgctgc cggccatttc cacgatccgc aggccaaagc
     1261 gcccgccatg ggcgacgatc tcgcccttgg cgatgacctt gccgttgacc atgatatcca
     1321 gcagctcgtc ggtcagccgg tcgagcatga cgacgctttc ctcgcccagc gagagcacgt
     1381 ccttcagctt catctgggcg cggcccagct ccaccgacag gcgcacatcg acatccttga
     1441 ggaaatcgaa gccgcggggc gggggggagg gaaagctcat gatgcggggc tcctgctgtc
     1501 gtcgaaggcg cgggtgagtt ggagggccac gcgttcgtcc agcgcgccga cagtgccatg
     1561 ggcaatggca acatcggcca cgctgagcgg caccgcgcgg gccaccgcca ccggcaggat
     1621 ctggccgggg gcaagcccgg cgatggtggc aaagggcacc tgcatgtcaa ccagagttgc
     1681 caccacttcc acgggcatat cggcaaaggg agtggcggtg gggctggcca tggggcggcg
     1741 gcgctggcgg cgggcggggg catcgctgcc gggcgctgcg cagccgaaca gggcaaacag
     1801 ggtgggctcg ggcagggcga tggtgaccag ccagggcagc accgagccgt cgtccacctc
     1861 gatcgtcatg gcgatcagcg gcaggctggc ggcaaagggg gccagcatcg ccaggctgcc
     1921 atcgcggcgg agcggcgtga tggcgggggc gccggcggtg cggccggcgc cggccggccc
     1981 cagcgcggca atcgccaggc caacctgttc ggccagcagc ttttccagcc gggcgatcat
     2041 cagctcggcg gccatgggga aggttttggg catgggcgcg ggcgcctcgc ccttgccgcc
     2101 aaaggcccgg tcgaccatgc gcagcaccgg ctcggcctcg atcgagacga gcagcggggc
     2161 agcccccgcg cccaccgcga acaggctgtt ggcggccagc ggcgcgatcg attgcatcag
     2221 cgcgccggca tcggtttcgc gcgggcgggc gcagtgcagc agcggcggct tggcgccctg
     2281 ctggctgaac agcatggggg caaaggcatc ggccagcttg cgttccagcc ggccgcccat
     2341 gcgttccagc agcggcagca gatccgcgcg cgtggggccc tggcgcagca gttcggggca
     2401 atggcgggcc agggtgcgct cggcaatgaa ggcgcgttcg ggtttcatct gcgggccgcc
     2461 ttactgcacg aggaaggtct tgaagtagac attgtccacg ccgccaaagc cctcttcctg
     2521 ggtcagcacg tggttgatgg catcggccag gcgcttttgc aggcgctcct tgccctggat
     2581 ggtgttgacg tcatcctcgg gtgtgtcggc cagcacttcc agcatggccg agcgcaccgc
     2641 cagctcatgc ttcttcagcc acatcagcac ccgcccgtcg cggtgggtag agcaggccag
     2701 cgagacctgg atcagcgcgc tggaattctt cagattggag gtgaaatcct ccgagaaatt
     2761 gtaatagctg gtcttgaagg ggctgccgcc atcgccttcc acttcgggcg tggcgccttc
     2821 cttgccgcct tcggccttgg gcgcgaaggg gtctgtatcg cccttgtgga tcagcttggg
     2881 cgaattgtcc tcgtgcacct tctcgccgcc gcccagcacg cccagcttga ccatggcaaa
     2941 ggcaccgccc ccgcccacgg ccagcatcgc cacggcgacg atgatcagca tcagcggccc
     3001 cttgcccttt ttcggcgggg cctcgctttc ggtcttgtcc ttgctcatcg gtggtctctc
     3061 aattggctgg gggcgtcagg ggtcaggcga aaatgccctg ggcggtggaa agggcggcgt
     3121 cggccgcatt gtcggtggca gtggcggcag gctgggcgct ggcgaggggc gccggggcgc
     3181 cgcgctgggc gccctggcct tggcgcgcgc tgtcctgtcc ggtggcggca ttgtggccgg
     3241 cgcccggccc gttttgcgta ttgccgctgt tctggccgcc gttctggccg gtgttgtcgg
     3301 aaggggcatc gccggtggcg ctgccggcgg cggccggggt gatcgccgcg ctgatggcgg
     3361 ccgcatgcac gctggtgagc gcgccggcgg cgtggggcgc ggcggcaagg gcggcctgcg
     3421 ccgaggcggc aaaggccgga tcgctgctgc tcatggccac gctgagggtg ccggtggcat
     3481 cggtctggaa gcgcagcgcc acctggccga aggcggcgtg ggaaatggcc gtttgcacca
     3541 cggccgggct gccggcgcgg gcggccgcgc gcgcttccac cagccggtcg accagcgctg
     3601 ccatatcctg gccggtggca gcggtgaccg aagagaccgg cacgctggcg ccagggccag
     3661 cggcagccgg gctggcggct gcctggctgg cagcgagggc cgctgcctga ttgacggcaa
     3721 ggccgggggc gggcgcagcg gtggccacag cgggctggct cgcgccgggc gtggcggcgt
     3781 gagtggtgcc cggggcggcg tttg
//
LOCUS       DASRYE010000272         7366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_157849,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000272 DASRYE010000000
VERSION     DASRYE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157849"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFF98_13915"
     CDS             <1..463
                     /locus_tag="VFF98_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU64775.1"
                     /translation="EGGALKGKYRASLAGEDMAIAAYNRAVIGAVKEVADALSQVATR
                     AEDAEAQHAITRGLDETLRLDHVRLASGLAGRLDALAAGQRLLSARQAETDIIADGLV
                     QRIRLAVALGGGFAPSAATATASADTPFMRPVSMRSGAPVQTVAPAHTAAR"
     gene            468..1640
                     /locus_tag="VFF98_13920"
     CDS             468..1640
                     /locus_tag="VFF98_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU64776.1"
                     /translation="MNENIPAATLLQPSRLARRKRALLLLGGVVVVALVGWGAYTLLT
                     GASQTTDDAYVGGNMVQITAREGGIVNAIHADATQTVTAGQPLIDLDGSTVDAQMAAA
                     EAQLAKSVRDVRQGFTRVDAAAAEVAQAQTQLATASSDLARRQAAVKAGAVSGEEAAH
                     AADAARNAGAALTLARARAAEAEASVAGTTIATNPAVLAAIAQIRQIAVARSHLHITA
                     PVDGVVAQRTVQLGQQVGPGTPMMTVVPLQQVWVDANFRETQLADIRIGQPVTLKADA
                     YGSAITFHGKVLGLAPGSGNAFALLPPQNASGNWIKIVQRLPVRIALDRQELADHPLR
                     VGLSVTVDVDTSSHEGKPLVGAPAAAVAQQSQPDVMPAVEERIHRIIADNSTGAAR"
     gene            1637..3193
                     /locus_tag="VFF98_13925"
     CDS             1637..3193
                     /locus_tag="VFF98_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZU64777.1"
                     /translation="MSSAAAQPAGPAPLHGAALAIAAISLALGSFMQVLDSTIANVSL
                     PTISGNLGESNETGTWVITAFAVANGVTVPLTGWLMRRFGVVTTFTASVALFTVASLL
                     CGIAWSLPSLIFFRLLQGAVSGPMIPGSQALLIAIFPGEKRAVALAIWSMTALVGPVM
                     GPILGGYISDHFHWGWIFLINVPVGIFTVSTLLARMRAYNTPPTKLPIDLGGLVLLVL
                     WVGALQVVLDLGKDADWFNSTPICVLAAISAVAFVAWVIWELTEEHPTVDLSLFKGRN
                     FTVGCGCFCLGYAFFFANNLLVPQWLQQQMGYTATWAGMVAAPAGVVAIALTPITARL
                     STKVDMRLMGSAALVAFGISYLLRASFSTAQTDFLHFVLPTLVQGMAMGTFFMSMTAI
                     CIDRLPPHRIPSATGISNFCRITGGSFSASIVTTWWDRRETLHQARLVDVAGPASPAF
                     THAMEQLRGLGLSTAQAAGVIDRQITNQAYVLGAQDLFWISGIGSLAMVGLLWFTRRP
                     MPPKGPVAAD"
     gene            complement(3243..3878)
                     /locus_tag="VFF98_13930"
     CDS             complement(3243..3878)
                     /locus_tag="VFF98_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HZU64778.1"
                     /translation="MPWNRDEMAARAARELEDGFYVNLGIGIPTLVANHVPAGMTVTL
                     QSENGMLGIGPFPYEGEEDADLINAGKQTISELPHSAYFDSAQSFAMIRGGHIDLTVL
                     GAMEVAENGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMEHTSKNGDPKFIPACTL
                     PLTGRNVVDMIVTDLAVFQRGDHHSPFRLVELAPGVSAEDVAAKTTAHYLV"
     gene            complement(3878..4588)
                     /locus_tag="VFF98_13935"
     CDS             complement(3878..4588)
                     /locus_tag="VFF98_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZU64779.1"
                     /translation="MAGKIYPSAEAALEGLLFDGMLIASGGFGLCGIPERLIDAIRQA
                     GTQALTFASNNAGIDNEGIGKLLRTRQVKKMISSYVGENKEFERQYLAGELEVEFTPQ
                     GTLAERMRAGGAGIPGFYTKTGVGTLVAEGKETKLFNGEEYVLEQGIFADLAIVKGWK
                     ADTTGNVVFRKTSRNFNVPAATCGRVTVVEVEEIVEAGALDPDAIHLPGVYVQRIALG
                     APYEKKIEFRTVRQRESA"
     gene            4723..5634
                     /locus_tag="VFF98_13940"
     CDS             4723..5634
                     /locus_tag="VFF98_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64780.1"
                     /translation="MNRIALYHLETLLWIARLGTFGAAARRLNTTQPTISARMRELEG
                     QLGYPLFRREGRRMLLTIRGRQLVRDCEPLWAALESTLAADGLAGATGVVRIGAGEIA
                     AASCLPGFLAEVEQAMPGVTLDVAINLTAHLVQELLGATTDMVFLAGPIASPGIRTAP
                     IGEVALRWLAAPATAARMAQGGYLAPVWSLPAHSPIHQLMLESLPQRSPGARPAPPVR
                     TCNNVRTLIDIVAQGSSMALLPETMTRARVAEGQLVEVRAAPARRIRFDVAIRAAESD
                     PVVLAIFERASRLEIDPEGGPEGRVAA"
     gene            complement(5631..6488)
                     /gene="purU"
                     /locus_tag="VFF98_13945"
     CDS             complement(5631..6488)
                     /gene="purU"
                     /locus_tag="VFF98_13945"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZU64781.1"
                     /translation="MSQTCTLSLQCADRPGLVALVAGFLAGRGGNILDAQQFNDRLND
                     RFFMRVVFALPENDTPDAWRAGFAPIAKGYGMDWRLRPAAERQKVLLLVSRFDHCLAD
                     LLYRWKTGELAMDVVGIVSNHPQTALTTSLIDGIPFHHLPITRDTKLAQEAEIKRIVA
                     ETGADLIVLARYMQILSDDMAVFLSGRCINIHHSFLPGFKGAKPYHQAHDRGVKMIGA
                     TAHYVTADLDEGPIIAQDVGVISHADEPEELVRKGRDIERRVLAEGVKAHLEDRVLLN
                     GARTVVFPG"
     gene            6635..7324
                     /locus_tag="VFF98_13950"
     CDS             6635..7324
                     /locus_tag="VFF98_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU64782.1"
                     /translation="MDSPLPLLLLPGLMCDGSVFAPQLAAFPQAQARPGWGPIASLTA
                     MAERALAEAPARFCLLGHSMGGRVALEMLRLAPERVARIALVSTGIHPTAPGEAEKRH
                     ALVDLGRTQGMAALVDSWLPPMIAPGNRSAHAAPLHAMCMAQGLDAFAAQVEALLARP
                     ATDAALAAIRVPTLVCTGALDLWSPADQHEVIAARIAGARLEIVEGAGHFLPVERAEA
                     FNAAIASWLAG"
BASE COUNT         1090 a         2557 c         2459 g         1260 t
ORIGIN      
        1 cgagggcggc gcgttgaaag gcaaatatcg cgcctcgctg gccggcgagg acatggcgat
       61 cgccgcctac aaccgggcgg tgatcggcgc ggtgaaggaa gtggccgacg cgctgtcgca
      121 agtggccacc agggccgagg atgccgaggc ccagcacgcc atcacccgcg ggctggacga
      181 gacgctgcgg ctggaccacg tgcggctggc cagcggcctt gccgggcggc tggatgcgct
      241 ggcggccggc cagcgcctgc tgagcgcccg ccaggccgaa accgacatca tcgccgacgg
      301 gctggtgcag cgcatccgcc ttgccgtggc gctgggcggg ggctttgcgc cctcggccgc
      361 caccgccacc gcttcggcag acactccttt catgcgcccc gtttccatgc gctctggcgc
      421 ccctgtgcag actgtcgccc ctgcgcacac tgccgcgagg taatccgatg aacgagaaca
      481 tccccgccgc cacgctcctg cagccctcgc gcctcgcccg gcgcaagcgc gcccttctgc
      541 tgctgggcgg cgtggtcgtc gtggcgctgg tgggctgggg cgcctatacg ctgctgaccg
      601 gcgccagcca gaccaccgac gatgcctatg tgggcggcaa catggtgcag atcaccgcgc
      661 gcgagggggg catcgtcaat gccatccacg ccgatgcgac gcagacggtg accgccggcc
      721 agccgctgat cgaccttgat ggatcgacgg tggacgcaca gatggccgcc gccgaggcgc
      781 agctggccaa atccgtgcgc gatgtgcgcc agggcttcac ccgggtggat gccgccgctg
      841 ccgaagtggc ccaggcgcaa acccagctgg ccaccgccag cagcgatctg gcccgccgcc
      901 aggccgcggt gaaggccggc gccgtctcgg gcgaggaagc cgcccatgcc gccgatgccg
      961 cccgcaatgc cggcgccgcg ctcaccctgg cccgcgcccg cgccgccgag gccgaggcca
     1021 gcgttgccgg caccaccatc gccaccaatc ctgccgtgct ggccgccatc gcccagatcc
     1081 gccagatcgc cgtggcgcgc agccatctgc acatcaccgc gcccgtggat ggcgtggtgg
     1141 cccagcgcac cgtgcagctg ggccagcagg tgggccccgg cacgccgatg atgacagtgg
     1201 tgccgctgca gcaggtgtgg gtggatgcca atttccgcga aacccagctc gctgatatcc
     1261 gcattggcca gcctgtcacg ctgaaggccg atgcctatgg cagcgccatc accttccatg
     1321 gcaaggtgct ggggctggcg ccgggcagcg gcaatgcctt tgccctgctg ccgccgcaaa
     1381 atgccagcgg caactggatc aagatcgtcc agcgcctgcc ggtgcgcatc gcgctcgacc
     1441 ggcaggaact ggccgatcac cccttgcgcg tgggcctctc ggtcaccgtc gatgtcgata
     1501 cctctagcca tgagggcaag ccgctggtgg gcgcgccggc cgccgccgtt gcccagcaaa
     1561 gccagcccga tgtgatgccc gcggtggagg aacgcatcca caggatcatc gccgacaaca
     1621 gcacgggcgc cgcgcggtga gcagcgctgc ggcccagcct gccggccctg ccccgctgca
     1681 tggggcagcg ctggccatcg cggcgatctc gctggcgctg ggcagcttca tgcaggtgct
     1741 cgattccacc atcgccaatg tctcgctgcc caccatttcg ggcaatctgg gcgaaagcaa
     1801 cgagacgggc acctgggtga tcaccgcctt tgccgtggcc aatggcgtga ccgtgccgct
     1861 caccggctgg ctgatgcggc gctttggcgt ggtcaccacc ttcaccgctt cggtggcgct
     1921 gttcaccgta gcctcgctct tgtgcggcat cgcctggagc ctgccctcgc tgatcttctt
     1981 ccgcctgctg cagggcgcgg tgtcgggccc gatgatcccg ggcagccagg cgctgctgat
     2041 cgccatcttc cccggcgaga agcgggcggt ggcgctggcg atctggtcga tgacggcgct
     2101 ggtggggccg gtgatggggc cgatcctggg cggctatatc tccgatcatt tccactgggg
     2161 gtggattttc ctcatcaacg tgccggtggg catcttcacc gtatccaccc tgctggcccg
     2221 catgcgcgcc tacaacacgc cgcccaccaa gctgccgatc gaccttggcg ggctggtgct
     2281 gctggtgctg tgggtgggcg cgctgcaggt ggtgctcgat ctgggcaagg atgccgactg
     2341 gttcaattcc acgccgatct gcgtgctggc ggcgatttcg gcggtggcct tcgtcgcctg
     2401 ggtgatctgg gaactgaccg aggagcaccc gacggtcgat ctgtcgctgt tcaaggggcg
     2461 caatttcacc gtgggctgcg ggtgcttttg cctgggctat gcgtttttct tcgccaacaa
     2521 cctgctggtg ccgcaatggc tgcagcagca gatgggctac accgccacct gggccggcat
     2581 ggtggccgcg cccgccggcg ttgtggccat tgcgctgacg ccgatcaccg cgcgcctttc
     2641 caccaaggtg gacatgcggc tgatgggcag cgccgcgctg gtggcctttg gcatcagcta
     2701 tctgctgcgc gccagcttct ccaccgcgca gacggatttc ctccatttcg tgctgccaac
     2761 gctggtgcag ggcatggcca tgggcacctt cttcatgtcg atgaccgcca tctgcatcga
     2821 ccgcctgccg ccgcaccgca ttccctcggc cactggcatt tccaatttct gccgcatcac
     2881 cgggggcagc ttttccgcct ctatcgtcac cacctggtgg gatcggcgcg aaaccctgca
     2941 tcaggcccgg ctggtggatg tggccggccc cgccagcccc gccttcaccc atgccatgga
     3001 gcaattgcgc ggccttggcc tcagcaccgc ccaggccgcc ggcgtgatcg accggcagat
     3061 caccaaccag gcctatgtgc tgggcgcgca ggatctgttc tggatcagcg gcatcggctc
     3121 gctggcgatg gtcggcctct tgtggttcac ccgccggccc atgccgccca agggccccgt
     3181 ggcggcggat tgacacggca ggccaggcgc gggtggagag cccgcgcctg gccttgcgcc
     3241 tctcacacca gataatgcgc cgtcgtcttg gcggccacgt cctcggcgct cacgccgggg
     3301 gccagttcca ccaggcggaa agggctgtgg tgatcgccgc gctggaagac ggcgaggtca
     3361 gtcacgatca tgtccaccac attgcggccc gtcagcggca gggtgcaggc ggggatgaac
     3421 ttgggatcgc cgttcttgga ggtatgctcc atcaccacga tgatcttctt gacgccggcg
     3481 acaaggtcca tcgccccgcc catgcccttg atcatcttgc cggggatcat ccagttggcg
     3541 atgtcgccgt tttcggccac ttccatcgcc cccagcacgg tgaggtcaat atggccgccg
     3601 cggatcatcg caaagctctg cgcgctgtcg aaataggcgg aatggggcag ttcgctgatg
     3661 gtctgcttgc cggcgttgat caggtcggca tcctcctcgc cttcataggg aaaggggccg
     3721 atgcccagca tgccgttctc gctctgcagc gtcacggtca tgccggcggg cacatggttg
     3781 gccaccagcg tgggaatgcc aatgcccagg ttcacataga agccatcctc cagctcacgc
     3841 gcggcgcggg cggccatttc atcacgattc cagggcatta tgcgctctcc cgctgccgca
     3901 cggtgcgaaa ttcgatcttc ttctcataag gcgcgcccag cgcgatgcgc tgcacataga
     3961 cacccggcag atggatggca tcgggatcga gcgcgccggc ctccacgatc tcttccactt
     4021 ccaccaccgt gacgcggccg caagtggcgg cgggcacgtt gaaattgcgc gaggtcttgc
     4081 ggaagacgac attgccggtg gtgtctgcct tccagccctt cacgatggcc agatcggcaa
     4141 agatgccctg ctccagcaca tattcctcgc cgttgaagag cttggtctcc ttgccctcgg
     4201 ccaccagcgt gcccacgccg gttttcgtgt agaagccggg gatgccggcg ccgcctgcgc
     4261 gcatgcgctc ggccagcgtg ccctgggggg tgaattccac ttccagttcg ccggccagat
     4321 actgccgctc gaactccttg ttctcgccca cataggagga gatcatcttc ttcacctgcc
     4381 tggtgcgcag cagcttgccg atgccctcgt tatcaatgcc ggcattgttg ctggcaaagg
     4441 tgagcgcctg cgtgccggcc tgccggatcg catcaatcag cctttcggga atgccgcaaa
     4501 ggccaaagcc cccgctggcg atcaacatgc catcgaacag cagcccctca agggcggcct
     4561 cggcgctggg atagatcttg ccggccatgc atcctcacct gtctggatat gggggcagtc
     4621 taacctgccg gattgatgcg ttccaatgat agtttttttg ggatatcgat aggcaatggc
     4681 aatgaatgag ccccccggcg ctgcttgcgg caggccccgc ggatgaaccg catcgccctc
     4741 tatcacctcg aaacgctgct gtggatcgcc aggcttggca cctttggcgc ggccgcccgg
     4801 cggctgaaca ccacccagcc caccatttcg gcccgcatgc gcgaactgga gggacaactg
     4861 ggctatccgc tgttccggcg cgaggggcgg cgcatgctgc tcaccattcg cgggcgccag
     4921 ctggtgcgcg attgcgagcc gctgtgggcc gcgctggaaa gcacgctggc ggccgacggg
     4981 ctggccggcg ccaccggcgt tgtgcgcatt ggcgcgggcg agattgccgc ggccagctgc
     5041 ctgcccggct tcctggccga ggtggagcag gcgatgcccg gcgtcacgct ggatgtggcg
     5101 atcaacctca ccgcccatct ggtgcaggaa ctgctgggcg ccacaaccga catggtgttc
     5161 ctggccggcc ccatcgccag ccccggcatc cgcaccgccc ccatcggcga ggtggcgctg
     5221 cgctggctgg ccgcgcccgc cactgccgcg cgcatggcgc aagggggcta tctggcgccg
     5281 gtgtggtcgc tgccggcgca ttcgccgatc caccagctga tgctggaaag cctgccccag
     5341 cgcagccccg gcgcccggcc cgcgccccct gtgcgcacct gcaacaatgt gcgcacgctg
     5401 atcgatatcg tggcgcaagg cagcagcatg gccctgctgc ccgagacgat gacccgcgca
     5461 agagtggccg aggggcagct ggtggaggtg cgcgccgccc ctgcccgccg catccgcttc
     5521 gatgtcgcca tccgcgcggc cgagagcgac cctgtcgtgc tggcgatctt cgagcgcgcc
     5581 agccggctgg agatcgatcc cgagggcggg ccagaggggc gagtagccgc ctagcccgga
     5641 aacaccaccg tgcgcgcgcc attgagcagc acgcggtctt ccagatgggc cttcacgccc
     5701 tcggccagca cgcgccgctc gatatcgcgg cccttgcgca ccagttcctc cggctcgtcg
     5761 gcatggctga tcacgcccac atcctgggcg atgatcggcc cctcgtcgag gtcggccgtt
     5821 acgtaatggg cagtggcgcc gatcatcttg acgccgcgat catgggcctg gtgatagggc
     5881 ttggcgccct tgaagccggg caggaaggaa tggtggatgt tgatgcagcg gcccgagagg
     5941 aaaacggcca tgtcatccga gaggatctgc atgtagcgcg ccagcacgat gaggtcggcc
     6001 ccggtttccg cgacgatgcg cttgatctcg gcttcctgcg cgagcttggt gtcccgcgtg
     6061 atcggcaggt ggtggaaggg gatgccgtcg atcaggctgg tggtcagcgc cgtttgcgga
     6121 tggttggaga cgatgcccac cacatccatc gccagctcgc cggtcttcca gcggtagagc
     6181 agatcggcca ggcaatggtc gaacctgctc accagcagca gcactttctg ccgctctgcc
     6241 gccgggcgca ggcgccagtc catgccataa cccttggcga tgggggcaaa gcccgcgcgc
     6301 catgcatcgg gcgtgtcgtt ttccggcagg gcgaagacga cgcgcatgaa aaagcggtcg
     6361 ttcagccggt cgttgaactg ttgggcatcg aggatattgc ccccgcggcc agccagaaaa
     6421 ccggccacca gcgccaccag cccgggcctg tcggcgcatt gcaggctcag tgtacaggtc
     6481 tggctcacgg ttgtcgtctc cttggttggc aggccgggat ggatgcgccg gggggatcgg
     6541 ccgggcgcgc ctgttgccgc cgccccatgc cgcgccggcc cgcgcttggc aagccatggg
     6601 cacaggcacg gcgatcggct atggcaaggc ccctatggat agccccctcc ccctgttgct
     6661 gctgcccggc ctgatgtgcg atggcagcgt gttcgccccc cagctggccg ccttcccgca
     6721 ggcgcaggcg cgccccggct gggggcccat cgccagcctc accgccatgg ccgagcgcgc
     6781 gctggccgag gcgccggcgc gcttttgcct gctgggccat tcgatgggcg ggcgcgtggc
     6841 gctggaaatg ctgcggctgg cgccagagcg ggtggcccgc atcgccctgg tttccaccgg
     6901 cattcacccc accgcccccg gcgaggcgga aaagcgccac gcgctggtcg atctgggccg
     6961 cacacagggc atggcagcgc tggtggacag ctggctgccg ccgatgatcg cacccggcaa
     7021 ccgctccgcc catgccgcac ccttacatgc catgtgcatg gcccaggggc tggacgcctt
     7081 tgccgcgcag gtggaagcgc tgctggcccg gcccgccacc gatgccgcgc tggccgcgat
     7141 ccgcgtgcca acgctggtgt gcaccggcgc gctggacctg tggagcccgg ccgatcagca
     7201 tgaggtgatt gccgcgcgca ttgccggggc gcggctggag attgtggaag gggccgggca
     7261 tttcctgccg gtggaacggg cagaggcctt taacgctgct atcgccagct ggctggctgg
     7321 ctgaaaacac cagcgggctg gcggttcccg gtccgccagc ccgcct
//
LOCUS       DASRYE010000273        24190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_211202,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000273 DASRYE010000000
VERSION     DASRYE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 24190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24190
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211202"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="grxD"
                     /locus_tag="VFF98_13955"
     CDS             complement(<1..297)
                     /gene="grxD"
                     /locus_tag="VFF98_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZU64783.1"
                     /translation="MATSDRIRTLVTDNEIVVFMKGEPAAPKCGFSAGVVKVMEAIGR
                     PYLGVDVLEDPFLREGLKAYSDWPTFPQVYVRGQFIGGCDIVREMYASGELQALF"
     gene            complement(311..550)
                     /locus_tag="VFF98_13960"
     CDS             complement(311..550)
                     /locus_tag="VFF98_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HZU64784.1"
                     /translation="MAMDADAIKARIEAALPDAEVLMIDLAGDGDHWAARVTSAAFAG
                     QSRIAQHKLVYAAIGADMGAALHALALQTFTPPTH"
     gene            complement(550..849)
                     /locus_tag="VFF98_13965"
     CDS             complement(550..849)
                     /locus_tag="VFF98_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZU64785.1"
                     /translation="MPTLTFKPSGTAITVAEGDNILAAMLAHGEKVAHKCDGKATCGE
                     CHIFLLEGRKGVSRLSPDENAKLDTMVGVGSKSRLACQCAVLGTEDITIEVLSFV"
     gene            complement(864..2039)
                     /locus_tag="VFF98_13970"
     CDS             complement(864..2039)
                     /locus_tag="VFF98_13970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZU64786.1"
                     /translation="MTNVVSIATGEPLQISLTDPDFTAADIAAVIAVLESPRLGEGPQ
                     VPAFERAFAQRLGRGAGVGLASAAMGLFLALRARGIGPGDEVILPAHGFRETGHAVLA
                     AGATPVFADIDYWSGCLAPAKAEARITPRTRALIAANAAGHPADWPALRALADAHGLF
                     LVEDSSEAIGSRHKAGEVGRFGDIALFDFRQGGVIACGEGALLLTDELDLAMAVRRMA
                     CRTLAERASVSAGGAPPLAVGMGELAAALGLSQLARLDVLLERRRGIQAMYDGFMQSF
                     EGIKPPYVAPDIEEVHWFLYVVHLGTRFSRSGRDAIIEDLRKEAMEAHAYSSPLHLQR
                     AYRDLGWKKGDLFVTEKVADRALALPFHAHLSPEQVAFMVARLKDASINTGAGAAIY"
     gene            complement(2036..2329)
                     /locus_tag="VFF98_13975"
     CDS             complement(2036..2329)
                     /locus_tag="VFF98_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64787.1"
                     /translation="MALDMQDIARIDALMAALGAGAGAGSGADHLREGLARLLPGLGQ
                     RSCDVDDVLEEPFRQLGAIGLHLLDTSGHCIRLTTDPAEATALLLATPQELRA"
     gene            complement(2339..2704)
                     /locus_tag="VFF98_13980"
     CDS             complement(2339..2704)
                     /locus_tag="VFF98_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HZU64788.1"
                     /translation="MSMAPQASAPQASPALGHPNEIDLLRITRAIGQRLRYRYVTPAV
                     SPVCGGYLVRSACCSRNVDADGGEIDVARILWCADPVGWLLYAKDHATGRWVEDSLFH
                     RLPELLARLNSDPDRRFWQ"
     gene            complement(2701..2964)
                     /locus_tag="VFF98_13985"
     CDS             complement(2701..2964)
                     /locus_tag="VFF98_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HZU64789.1"
                     /translation="MIPLRAPVFRWGQQVRTALALVNDGSHPGFAPEAVIVPAGAVGE
                     IVNVGHHEESDTPVYLVDFAAGTPAPCVVGCLEEELEELEAAP"
     gene            complement(2961..3305)
                     /locus_tag="VFF98_13990"
     CDS             complement(2961..3305)
                     /locus_tag="VFF98_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HZU64790.1"
                     /translation="MSNIVRDSDVVELNGPPAFRYGEKVRALRHVRNDGTYPGKEIGD
                     LLVRKGEEGVVSSIGTFLQQFYIYGVDFPQSGHKVGMKRRELAALWEAQEAEDELPCT
                     LPPARPATEARP"
     gene            complement(3298..3822)
                     /locus_tag="VFF98_13995"
     CDS             complement(3298..3822)
                     /locus_tag="VFF98_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64791.1"
                     /translation="MSGRGSLLADGLGLLDDPLLGGGLPWAAQAHLDKAALCYHQSDV
                     AETHLRDAGRLAPGHAAVLIALYRFYFYKGRLVEALDVARECLAKALAENVLGEDWRA
                     VGPDDADFADWGALRPRFFLFTLKAYAYLNMRLGCYGEGRRAAEKLLFLDRGDRIGAR
                     VLVDVLNRMEAGDE"
     gene            complement(3819..4046)
                     /locus_tag="VFF98_14000"
     CDS             complement(3819..4046)
                     /locus_tag="VFF98_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZU64792.1"
                     /translation="MPYRIVASQCTNCSACEAQCPNEAISEKGNTFVIDPVKCTECAG
                     HFDTPQCVAICPVDLTCIIDTSLPRYQGQPA"
     gene            complement(4062..5630)
                     /gene="nifB"
                     /locus_tag="VFF98_14005"
     CDS             complement(4062..5630)
                     /gene="nifB"
                     /locus_tag="VFF98_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HZU64793.1"
                     /translation="MASSVPGSFAGIAENSEPVVDIADVMQKIAEHKGCGTSSDGGKA
                     SCGSSAGPADMAPEIWEKVKNHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCA
                     NESRPGVVSERLTPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPKKTFETFRLISD
                     VAPDIKLCLSTNGLALPDWVDEIAKYKVDHVTITINMVDPEVGQHIYPWIFWDHKRVE
                     GLEAARILSERQLLGLEMLTERGILAKINSVMIPGVNDMHLVEVNKAVKSRGAFLHNI
                     MPLISAPEHGTVFGLNGQRGPSAQELKALQDACEGDMNMMRHCRQCRADAVGLLGEDR
                     SAEFTTDKIMAMEVEYDAEGRKAYQQQVEAERQAKVAARAGELDGLEAAGDIRVLVAV
                     ATKGHGVINEHFGHAKEFQIYELSGTGAKFVGHRRVDLYCQGGFGEEDSLDTIIKAIN
                     DCHAVFVAKIGGCPKADLTAAGIAPVDAYAHEYIEASAIVWFKDYLAQVASGAIVHAA
                     RGDAAIRQGALLAS"
     gene            complement(5890..6735)
                     /locus_tag="VFF98_14010"
     CDS             complement(5890..6735)
                     /locus_tag="VFF98_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HZU64794.1"
                     /translation="MSGLTLENPRALLAEVAEGLKAGQVVPYLGPGISALGAPPAPMT
                     PEDLAGFFASKTTLPRRAVGNAWAAAQYIESHRFRDTLTAWMKEAFARPVPPSAFHGW
                     LAGLGLPLIVDVWYAGEMRSALNAAGADWAEVQGISRAGIGEDRWFRCYGPDGAPLDA
                     VRAHMAPTLLYTPHGGAQPAGNFLISDADYVEVLTEIDIQTPIPEEVRRRRLGRSFLF
                     AGCRFHDQMLRSYARQIIKRSSAKHYAIVDPGQLTRNEKRFLDEHDFTLIALPLGEAL
                     ATLGG"
     gene            complement(6737..6943)
                     /gene="nifT"
                     /locus_tag="VFF98_14015"
     CDS             complement(6737..6943)
                     /gene="nifT"
                     /locus_tag="VFF98_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HZU64795.1"
                     /translation="MIRRSEGALSAYVPKKDLEEPIVETERAALWGGWVRLANGWVLE
                     LPEMAEDTRLPITVEARKRQDGEG"
     gene            complement(6949..7848)
                     /locus_tag="VFF98_14020"
     CDS             complement(6949..7848)
                     /locus_tag="VFF98_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HZU64796.1"
                     /translation="MGAAAEAGEDGLGPPLDWQGRPVRCRECAHIAINQMGLCARGKA
                     CIRDRRARRVERFLRGNPDLADSYLDHPYFEVRAGAARYCNPFRLPALLRDPEPEVRA
                     MATLRLPEERIREMIRDEDMDVRIAAAARLSGPALLSAIHDSAYMVRLTAVRRMPVEL
                     LPLVRHDPDPEVRQWVARRIDIRALPDMAFDPDSQVRRQVAERLPADQLVILAQDSDL
                     AVRFCVAERAVEEDLFALVDDPEEMVRALARARIAAVPALAGRLEAALCRRPALRLLP
                     AIDTDAASAGAAGHAGPETEQER"
     gene            complement(7864..8058)
                     /locus_tag="VFF98_14025"
     CDS             complement(7864..8058)
                     /locus_tag="VFF98_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZU64797.1"
                     /translation="MAYKIIASQCTGCSACEFECPNAAIRMKGDVFIIDPAKCTECEG
                     HFDEAQCNVVCPVPNTCVPA"
     gene            complement(8231..8662)
                     /locus_tag="VFF98_14030"
     CDS             complement(8231..8662)
                     /locus_tag="VFF98_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64798.1"
                     /translation="MQDGTFPSPDYPEVTAPRRDAGRGDAYARDCPTRALLDRVGDKW
                     SVLILLLLGEGAMRFNGLKRRIGGVSQKMLSQTLRALERDGLLAREVLPTTPVSVTYR
                     ITPLGRELLAALRLMIDWAETRMGAVVAAQAAYDAACGRAP"
     gene            8718..9326
                     /locus_tag="VFF98_14035"
     CDS             8718..9326
                     /locus_tag="VFF98_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU64799.1"
                     /translation="MKIAVLGASGRAGSEITRELAARGHQVLAIARKPEAIAALPGVV
                     AQAGDASDPAALAALIQGYDAVISALHFDVSAATLLAALKQAGVGRLLVTGGAASLEV
                     APGVRVIDTPDFPAAWKTAALGGIAFLDSLRGESAIDWTFFSPAALIFEGPRLGHYRP
                     GTDQLVTDEAGESRISFADYAIAMVDELEQHRHSRARFTAAY"
     gene            9438..11000
                     /locus_tag="VFF98_14040"
     CDS             9438..11000
                     /locus_tag="VFF98_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZU64800.1"
                     /translation="MDSGLGDVGLYGFIAVLLAALGFVAYSIYADAVAAGLQTMALLP
                     FLLLFVALLIALGFEFVNGFHDTANAVATVIYTHSLPAHVAVVWSGFFNFLGVMASTG
                     AVAFGIVSLLPVELILQVGSNAGFAMVFALLIAAIIWNLGTWYLGLPSSSSHTLIGSI
                     IGVGVANAIMHGRAGTAGVDWTKATEIGQALLFSPLIGFGAALLLFIAAKALIRVKAL
                     YQEPQGDMPPPLWIRGLLVLTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNRA
                     VPAHYEAQFHIASGQAEAALAAHAGGVAAPADIRAEVTHYIADKKLSPATVPALGALV
                     ADIDRQVGAYHSLAKVPAAATGNMRNDMYLAASAIKRLGKEKAVALSAAEAKTLDGYK
                     KDQLDKATQFIPTWVKVAVAIALGLGTMIGWKRIVVTVGEKIGKTHLTYAQGAVAEMV
                     TMATISAADNFGLPVSTTHVLSSGVAGTMAGNGSGLQLGTLRNLLIAWVLTLPVSILL
                     SGGLYALLSLVV"
     gene            11172..13580
                     /locus_tag="VFF98_14045"
     CDS             11172..13580
                     /locus_tag="VFF98_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64801.1"
                     /translation="MEEIATRSPHSGSIALALGCLGLFAPAFAAEARAADADQPPAGT
                     DAGSNLGGVTVTDTAIDEGSYRTERLASPKSTAALVDTPRSITVISHQLLEDTNSTTL
                     SEALRTVPGITLGAGEGGNPLGDRPFIRGADSQNSIYLDGVRDMASQTREAFDVESIE
                     VVKGSDSVTNGSANAGGSINMISKAPEAKRFVQGDASFGNAAYKRFTLDLNQPLSDFV
                     GFRLNAMYHYQGVAGRDATWQKRWGIAPSIKFGMNGPTSLQLDWYHMHSNELPDEGIP
                     YYYTLSNQPNTNNQTAPAGTDVRARGAFYGLAGRDFRTTNTDNVSARFEHHFDSGLLL
                     RNTARYSHTTQEYLWTQPDDSKGNVASYGTVSRRANSRYSYENGIVDQVDLSGKFATG
                     ALKHSFAAALEYNWQKSAYGSYYANGTTGAALPTASTCLTATSNGNANCTSLANPNPY
                     DAYSGTLQKGPANSMTLADSSTLSASLFDTITLNDKWILTLGGRFDHYVTHASAGLSA
                     TTYPNFVGTRTWLAKTDDLFTYQAGLAYKPRRNGTIYISTSTAAIPPGSFLAQGSEDN
                     SLTTTTGITVNDLKVQKTTSYEVGTKWDLFHNALALSFDLFQTRTTNARTTAGDGTSV
                     AFVGLKRVRGIELGLSGNITPAWSVFGGYTYMDSIVLDAGTTKTTTGTYVAYAPAAAT
                     GKPFPNTPKNSFTLFTTYKATHRLTLGGGAIYMSKVYGGFSDSRQANADGTVTILKTR
                     ATYVPAYWRFDANASYKLNDFLTLKLSALNLTNKLYYDQAYATHYAHQAAGRTVIGTV
                     SVKY"
     gene            13580..14392
                     /locus_tag="VFF98_14050"
     CDS             13580..14392
                     /locus_tag="VFF98_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HZU64802.1"
                     /translation="MSDCPHNAGPVSAQGGASLPLPAGVPADAEAWRALLSGPAEAAA
                     QALYQAALAGDAEAQLLYGQHLLDGAGVVCDPAAALGWFHAAAQAGLPAAINMVGRCL
                     DQGWGTPPDPAAAAAWFEAAAARGLDWGLYNLATALALGRGLAQDRPRALALFRRAAA
                     MGHAKSMNMVGSFHEDGWSVPPSRLLAAYHYARAAEGGDFRGAFNHARLLIEEDQSEA
                     ALPWLNRAHAAGTARFAGQMAAWLAARPEPALRQAAARWQAETPAESHPRAA"
     gene            14382..15023
                     /locus_tag="VFF98_14055"
     CDS             14382..15023
                     /locus_tag="VFF98_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZU64803.1"
                     /translation="MPPEATKSSRRPRSARQFWMRQLHSWHWISAALSLVGMLLFALT
                     GLTLNHADAIPAHPQVTDRAGQLPASLLPLLAHPASADAPLPAAVAAAAAEATGLNPA
                     GHPAEWSDDAAYVALPRPGGDAWVSIARKDGQITSEVTRRGWISLLNDLHKGRNAGPA
                     WGWFIDLFALACVIFTGTGLLLLQLHARHRPSTWPIVGAGLLIPVLIAHFLVP"
     gene            15048..15530
                     /locus_tag="VFF98_14060"
     CDS             15048..15530
                     /locus_tag="VFF98_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HZU64804.1"
                     /translation="MRNPSLLAPLAAGLVATPALAGTVSVQIPRVDVAEYHRPYVAIW
                     LEPAAGGSPRTLAVWYDAKHRGEPGTKWLSELRGWWRVAGRAMAVLPEGLSGATRAPG
                     QYAIPLPADLKPGAYTLKVEAARETGGRELVSVPITLPRGGGRAAGHGELGAVAVALP
                     "
     gene            15582..16520
                     /locus_tag="VFF98_14065"
     CDS             15582..16520
                     /locus_tag="VFF98_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HZU64805.1"
                     /translation="MPMSSSRLRLLLAGALLAGPMLAPAAAFAHRPWLLPSATSFSGE
                     DPWVSVDAAVSNDLFFADHFPMQTAQIHVEAPDGAAGQIENAATGRYRSTFDVHLTRP
                     GTWKIGTDSTMLTGSFVADGVAHRVGRRPGGPAGPGGPGGPGGPGGERPGGGKPEQGN
                     GPAFAGPPVAAEDIPANATDIKLVEAISRNEIFVTSGQPTTGVFAPTGKGLELSPITH
                     PDDLVAGEVAKFRFLIDGRPAAGLKLTIIPGGKRYRDAEGGFAVTTGADGVASITWPS
                     AGMFWLNAVATDNHPAAPRATERRMSYTTTLEVMAP"
     gene            16523..17521
                     /locus_tag="VFF98_14070"
     CDS             16523..17521
                     /locus_tag="VFF98_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZU64806.1"
                     /translation="MASLPAAAPAAARIALPARIDAAAVQAPPAAGTLHRLRGETMGT
                     LWRATLAAPGMAPAALEGLITRRLAGIVDEMSHWQADSCLGRFNRLPAGGWMALPADF
                     ARVMAAALALAEASGGAFSPALGRLVDLWGFGPPGPVPAPPAAQPLARAHADADWRRL
                     AFEPAPARLRQPGRLALDLSAIAKGFAVDALAEALVGAGIAHFAVEVGGEWRGSGVQP
                     GGQPWWVDLETPPGAALAPLRIALHGLSVATSGTYLRGDHNLDPRSGRPAAGGIVSCS
                     VVHASAMIADGWASALTVLGPEAGLEMANRQGLAARLVLRGGAEVISTPLRALLAD"
     gene            17623..18408
                     /locus_tag="VFF98_14075"
     CDS             17623..18408
                     /locus_tag="VFF98_14075"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZU64807.1"
                     /translation="MAESTALLRFVDTPLAALGAPGPQRRAPAARAPQAPFLRTAPSY
                     AAPRPRQRLAAAIVSAGTVAALFSAIAVMNAMHVAHREPRIVAINLTPPMAPPQPPKA
                     KPAPTPREAVAQPATPAPAQPAATAAPAPIAAALPVNSAPPAPPAPAAAPAPAPAPAA
                     APAAPAMAEAGDLSARMLSFVAPTYPIDSRRAHEEGTVVLSLLLGTDGRVQEIQVAHT
                     SGSPRLDSAALAAVRKWRWAPVVRDGQPVLVRGTVRIPFLLKR"
     gene            complement(18418..19050)
                     /locus_tag="VFF98_14080"
     CDS             complement(18418..19050)
                     /locus_tag="VFF98_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZU64808.1"
                     /translation="MSQVVFPQTPAHAGRLDAAGAPDHPLALVRAWYDHVLAIGAEQP
                     AYVTFATSSVAGVPSSRTVQMLEVEAGALLFTTNFGSRKGLEMLATGRAAVSIYWRET
                     AQSINLTGTIDIAGEAECDRRFAEEDRAVMAARTVSFHGRPLVDEGAQLAAFHALLES
                     DAPIARPDYWKWFRLLPDAVTFWEGCPGALNRRLHYALEGARWQRGAIEA"
     gene            complement(19050..21359)
                     /locus_tag="VFF98_14085"
     CDS             complement(19050..21359)
                     /locus_tag="VFF98_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64809.1"
                     /translation="MIAALWAGCLGTAAQARAGEAAAHEAAADEAGADNAGASEAPAA
                     QGDILVTAERRSSKLQSTALAITVLPSNILDSANVQDVTDLSGMAPGLTVRKTGGRET
                     VPSIRGIGSGTPENPGSTAPGVAMFIDGVYVGDSVSLGHSLFDLDHMEVMRGPQGTLY
                     GVSAIGGALLLVSNQPKLDKISGNFDVSLGDYNYIRARGAINVPLSDTVAVRLSGQRL
                     SHDGFTTNTVNPGYDLDDADDTAVKGAVLWQPSSTFSATLTGEWTRQHDHGPAQKWLN
                     DPNPDPRVLSQDYLSKNYYKSTMVHLNLNWDLPVFSIRSVSAYRSYKSEFAENVTFTT
                     FDRYGAYEYVPTWNHNEESASEEIDFISKPGSKLSWTAGVFFMRRVAKDYTLEYYGTN
                     ASDATPAYVDPSTPSADIPSNLSYGNWTRDVRLVVEPFVQFSYPVTDKLKLTAGARYN
                     YESHDHLSHNFSEYGNDPLFGLKDETSVPTWRAELAYTLSPQNMVYVSAATGYKAGGA
                     NGNAGTALVPYTFKDEHNTAFEIGSKNFFFDKKLRLNMAGFFYIYKNMQYIETDPIPF
                     DQGMSNIPLVHIWGGEAEASYTGMAGRLHINGNLTVEQGKVVSHTNVLYPTIVNNAYS
                     SVGACAYGGQYYSSSCWAGVIASAKDIYGNTPPNMPEISASLDVSYDIAFMGGKLTPW
                     IQYVHVGARQARIFNEAYLDHEPAYNLINLSLAYEPPHSRFHLALSVSNATDVAGVSS
                     RYTSPYEQGLTSQQYIPPRQIIGTIGYSF"
     gene            complement(21585..22067)
                     /gene="cynS"
                     /locus_tag="VFF98_14090"
     CDS             complement(21585..22067)
                     /gene="cynS"
                     /locus_tag="VFF98_14090"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HZU64810.1"
                     /translation="MKREQLVEKILDRKRQEGWSWRHICDEIGGLSPVLIVGALLGQM
                     KLPKPLAANAARLFGLDGQEERMLNEVPHRGSPMPPTDPLIYRFYELVMVNGPAWKAL
                     IEEEFGDGIMSAIDFDMEMERLPHPKGDRVKFTLSGKFLPFKYYGSGQGIPEYGFGDT
                     "
     gene            22202..23128
                     /locus_tag="VFF98_14095"
     CDS             22202..23128
                     /locus_tag="VFF98_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZU64811.1"
                     /translation="MPAIPQPPLRDHGRFAYSPIHARPAYDWQRPEGGSARLAVYIGF
                     NIEHFAFGEGLGARLGAFAGEPDVLNYSWREYGNRVGAWRCLDLFDALGLPTGCLINT
                     ALYDHCPELVAAFARRGDELIGHGHSNAARQSDLPEAEERALLAACRARIASHSGTAP
                     DGWLSPWIAETHATPDLLAETGYRYTLNWAHDDQPTRLRTRGGEGLWSIPYPQELNDI
                     PMIIARQLDGQAFARMIIDNFLEMREQASQQPLVMGIALHPYLVGQPYRLRHLRGALE
                     EICRARDAGDVWLATPGAICRQMEALAAEGRV"
     gene            complement(23125..23862)
                     /locus_tag="VFF98_14100"
     CDS             complement(23125..23862)
                     /locus_tag="VFF98_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZU64812.1"
                     /translation="MIAQPARRLLAPAITFLLLGLAWQWVAGRHGAILPSLLDVAGDL
                     AARPAFYAANVWTTASVALAGLVLGGLAAVLLAICFVQFRFLRDAIFPVALLLNVTPI
                     VAIAPALIVVFGFNATPHVIVAAIAAFFPMLINAMAGLQAIDAEALEVFRALAASRLD
                     IFLRLRLPSSLPHLFAGGRLAIVAAVVGAIVSEFTGTADGIGAAIVLATAWLNLVQMW
                     AAIFCSAALSLALIGLVDLAERLVVRR"
     gene            complement(23859..>24190)
                     /locus_tag="VFF98_14105"
     CDS             complement(23859..>24190)
                     /locus_tag="VFF98_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64813.1"
                     /translation="VVGAPLMFMDEPFSALDELTRNALCWRLLEVWEKHRKTVLFVTH
                     SAFEAVLLSDRVLVMSPGPGRVHADIAIDLPRPRRWEMTETAEFAALVAQVKHALWAG
                     LGEGERP"
BASE COUNT         3945 a         8589 c         7685 g         3971 t
ORIGIN      
        1 aaacagcgcc tgcaattcgc cgctggcata catctcgcgc acaatatcgc agccgccgat
       61 gaactgcccg cgcacataga cctgggggaa ggtgggccag tcggaatatg ccttcagccc
      121 ctcgcgcagg aacgggtctt ccagcacatc aacgcccaga tagggccggc cgatggcttc
      181 catcaccttc accacgccgg ccgaaaagcc gcatttgggc gcggccggct cgcctttcat
      241 gaacaccacg atctcattgt cggtcaccag tgtgcggata cggtcggatg tagccatggc
      301 ggttctcctg tcagtgggtg gggggcgtga aggtttgcag ggcaagggcg tgcagcgccg
      361 cgcccatatc ggcgccgatc gcggcataga cgagcttgtg ctgggcgatg cggctctggc
      421 cggcaaaggc cgcgctggtc actcgcgcgg cccagtggtc gccatcgccg gcaaggtcga
      481 tcatcagcac ttcggcatcg ggcagcgcgg cctcgatgcg ggccttgatg gcatcggcat
      541 ccatcgccat cacacgaagc tcagcacttc gatggtgatg tcctcggtgc ccagcaccgc
      601 gcactggcag gccagccgcg atttggaacc cacccccacc atcgtgtcga gcttggcgtt
      661 ttcatccggg ctcagccgcg agacgccctt gcgcccttcc agcaggaaga tgtggcattc
      721 gccgcaagtg gccttgccat cgcatttgtg ggccactttc tcgccatggg ccagcatggc
      781 cgcgaggatg ttgtcgccct cggcaacggt gatggcggtg cccgatggct tgaaggtcag
      841 ggtaggcatg ggtgtgctcc ggttcagtag atagcggcgc cagcgccggt gttgatggaa
      901 gcgtccttca gccgggcaac catgaaggcc acctgctccg ggctgagatg ggcgtgaaac
      961 ggcagggcca gcgcgcgatc ggccaccttt tcggtgacga acagatcgcc cttcttccag
     1021 cccagatcgc gataggcgcg ctgcaggtgc agcgggctgg aataggcatg ggcctccatc
     1081 gcctccttgc gcagatcctc gatgatcgca tcgcggcccg agcgcgagaa gcgcgtgccc
     1141 aggtggacga catagaggaa ccagtgcact tcctcgatat cgggggccac atagggcggc
     1201 ttgatcccct cgaagctttg catgaagcca tcatacatcg cctgtatgcc gcgccggcgc
     1261 tccagcagca catccagccg ggccagttgc gacaggccaa gggccgctgc cagctcgccc
     1321 atgcccaccg ccagcggcgg cgcgcccccg gccgagaccg aggcccgctc ggcaagcgtg
     1381 cggcaggcca tgcggcgcac cgccatcgcc agatcgagct cgtcggtcag cagcagcgcg
     1441 ccttccccgc aggcgatcac cccgccctgg cgaaaatcga acagggcaat atcgccaaag
     1501 cggcccacct cgcccgcctt gtggcgcgag ccgatcgcct cgctgctgtc ctccaccagg
     1561 aacaggccat gggcatcggc cagcgcgcgc agggccggcc aatcggccgg gtggccggcg
     1621 gcattggcgg cgatcagcgc gcgggtgcgc ggggtgatgc gcgcctcggc cttggccggc
     1681 gccaggcagc ccgaccagta gtcgatatcg gcgaacaccg gcgtggcgcc ggccgccagc
     1741 accgcatggc cggtttcgcg aaagccatgg gcgggcagga tcacctcgtc gcccgggcca
     1801 atgccgcgcg cacgcagggc cagaaacagc cccatcgccg cgctggccag ccccaccccc
     1861 gcgccgcgcc ccagcctttg ggcaaaggcg cgctcgaaag cgggcacctg aggcccctcg
     1921 cccagccgcg ggctttccag cacggcgatc accgcggcga tatccgcggc ggtgaagtcg
     1981 gggtcggtca ggctgatctg cagcggctcg ccagtggcga tggagacgac attggtcatg
     2041 cgcgcagctc ctgcggggtg gccagcagca gggcggtggc ctcggccgga tcggtggtga
     2101 ggcggatgca gtggccgctg gtatccagca ggtgcaggcc gatggcgccc agctggcgga
     2161 agggttcctc cagcacatca tcgacatcgc agctgcgctg gcccagcccc ggcaacaaac
     2221 gcgccagccc ttccctcagg tgatctgcgc ccgaccctgc cccagcccct gctcccagcg
     2281 ccgccatcag cgcatcgatc cgggcgatat cctgcatgtc cagcgccatc gcttcctcct
     2341 attgccagaa ccggcgatca ggatcgctgt tcagccgcgc cagcaattcg ggcaggcggt
     2401 ggaacaggct gtcttccacc cagcggccgg tggcatgatc cttggcatag agcagccagc
     2461 ccaccggatc cgcgcaccac aggatgcgcg ccacatcgat ctcgccgcca tcggcatcga
     2521 cattgcgcga gcagcaggcc gagcgcacca gatagccgcc gcaaacaggg cttaccgccg
     2581 gcgttacata gcgatagcgc agacgctggc cgatcgcccg ggtgatgcgc aaaaggtcga
     2641 tctcgttggg atggcccagg gccgggctgg cttgcggggc gctggcttgc ggagccatgc
     2701 tcatggcgcg gcctccagct cttccaactc ttcctcaagg cagcccacca cgcaaggcgc
     2761 cggcgtgccg gccgcgaaat ccaccagata aaccggcgtg tcgctctcct catgatggcc
     2821 gacattgacg atctcgccca ccgcgcccgc cggcacgatc accgcttccg gcgcaaagcc
     2881 cgggtggctg ccatcgttca ccagggcaag cgctgtgcgc acttgctggc cccagcgaaa
     2941 caccggcgcg cgcagcggga tcatggccgc gcctccgtgg ccgggcgggc aggcggcagg
     3001 gtgcagggca gctcgtcctc tgcctcttgc gcctcccaca gggcggccag ctcgcgccgt
     3061 ttcatcccca ccttgtggcc gctttgcgga aaatccacgc catagatgta gaattgctgg
     3121 aggaaggtgc cgatggaaga gaccaccccc tcctcgccct tgcgcaccag cagatcgccg
     3181 atctccttgc cgggataggt gccatcgttg cgcacatggc gcagcgcgcg caccttctcg
     3241 ccatagcgga aggccggcgg gccgttgagt tcgaccacat cgctgtcgcg cacgatgtta
     3301 ctcatcgccc gcctccatcc ggttgagcac atcgaccagc accctggcgc caatgcgatc
     3361 gccccgatca aggaacagca gcttttcggc cgcccgccgc ccctcgccat aacagcccag
     3421 ccgcatgttg agataggcat aggccttgag cgtgaagagg aagaagcgcg gccgcagcgc
     3481 cccccaatcg gcaaaatcgg catcatcggg ccccaccgcg cgccaatcct cgcccagcac
     3541 gttttcggcc agcgccttgg ccaggcattc gcgcgccaca tccagcgctt ccaccagccg
     3601 ccccttgtag aaatagaagc ggtagagggc gatcagcacc gcggcatgac caggcgccag
     3661 ccggccggca tcacgcagat gcgtctcggc cacatcgctc tggtgatagc acagggcggc
     3721 cttgtcgaga tgcgcctggg cggcccaagg caggccaccg cccagcagcg gatcatcgag
     3781 caggcccagc ccgtcggcca gcaggctgcc ccgcccgctc atgccggctg cccctgatag
     3841 cgcggcaggc tggtatcgat gatgcaggtc aggtccaccg ggcagatcgc cacgcattgg
     3901 ggcgtgtcga aatggccggc gcattcggtg catttaaccg ggtcgataac gaaagtgttc
     3961 cccttctcgc tgatcgcctc attggggcat tgcgcctcgc aggccgagca attggtgcac
     4021 tggctggcaa cgatcctgta gggcatgggc gcctccctcc ctcagctggc caacagggcg
     4081 ccctggcgga tggcggcatc gccgcgcgcc gcatggacga tcgcgccgct ggccacctgc
     4141 gccagatagt ccttgaacca gacgatcgcc gaggcctcga tatattcatg ggcataggca
     4201 tcgaccggcg cgatgccggc ggcggtcaga tccgccttgg ggcagccgcc gatcttggcg
     4261 acgaacaccg cgtggcagtc gttgatcgcc ttgatgatgg tatcgaggct gtcttcctcg
     4321 ccaaagccgc cctggcaata aagatcgacc cggcgatggc cgacgaattt ggcccccgtg
     4381 ccagacagtt cgtagatctg gaattccttg gcatggccaa agtgttcgtt gatcacgcca
     4441 tggcccttgg tggccacggc caccagcacg cggatatcgc cggctgcttc cagcccgtcc
     4501 agctcgccgg ccctggccgc caccttggcc tgcctttcgg cctccacctg ctgctgatag
     4561 gccttgcggc cttcggcgtc atattccacc tccatcgcca tgatcttgtc ggtggtgaac
     4621 tcggccgaac ggtcctcgcc caagaggccc accgcatcgg cgcggcactg gcggcagtgg
     4681 cgcatcatgt tcatgtcgcc ctcgcaggca tcctgcagcg ccttcagctc ctgcgccgaa
     4741 gggccgcgct ggccgttgag gccgaacacc gtgccatgtt cgggcgcact gatcagcggc
     4801 atgatgttgt ggaggaaggc cccgcggctc ttcaccgcct tgttcacctc gaccaggtgc
     4861 atgtcgttga cgccggggat catcactgag ttgatcttgg cgagaatgcc gcgctcggtc
     4921 agcatttcaa ggcccagcag ctggcgttcg gacaggatgc gcgccgcctc caggccttcc
     4981 acccttttgt gatcccagaa gatccacgga tagatgtgct ggcccacctc gggatcgacc
     5041 atgttgatgg tgatcgtcac atggtccacc ttgtatttgg cgatctcgtc cacccagtcg
     5101 ggcagggcca ggccattggt ggaaagacac agcttgatat cgggcgccac gtcgctgatg
     5161 aggcggaagg tctcgaaggt cttcttcgga ttggccagcg gatcgcccgg gccggcaatg
     5221 cccagcaccg tcatctgcgg gatggtggag gcgacggcca gcaccttttt cgcggcctgc
     5281 tcaggggtca ggcgttcaga cacgacgccc gggcggcttt cattggcgca atcatacttg
     5341 cggttgcagt agttgcactg aatgttgcag gccggcgcca ccgccacatg catgcgcgca
     5401 tagtggtggt gggcttcctc ggaatagcag gggtggttct tcaccttttc ccagatttcg
     5461 ggcgccatgt cggcaggccc ggcgctggat ccgcagctgg ccttgccgcc atcgctgctg
     5521 gtgccgcagc ccttgtgctc ggcgatcttc tgcatcacat cggcaatatc gaccaccggc
     5581 tcgctgttct cagcaatgcc ggcgaaactc cccggaacgg aactggccat gagctgctcc
     5641 ttttcgtgtg tggcggccct ttggaaggcg ggccacctgg ctgcccacct ctatcgcaaa
     5701 gcccgtgcca ggatcggcat gtgccgcttt tccgccattt cccgtcccat ccgggccgac
     5761 atgcgcatgt gggttttgcg ccaatgttgc gaacatgaca gagcagcgcc ccgcccccac
     5821 acgcacgccg cgcacgcaaa aaagggcgcc ggcccgggta gaccgacgcc cagttttggg
     5881 ccgccccgat cagccgccca gcgtggccag tgcctcgccc agcggcaatg cgataagggt
     5941 gaaatcatgt tcgtcgagga agcgcttttc gttgcgcgtc aactgtccgg gatcgacgat
     6001 ggcatagtgt ttggccgagg agcgcttgat gatctggcgc gcataggagc gcagcatctg
     6061 atcgtggaaa cggcagccgg cgaacagaaa gctgcgcccc agccggcgcc ggcgcacttc
     6121 ctcggggatc ggcgtctgga tgtcgatctc ggtcagcact tccacataat cggcatcgga
     6181 aatgaggaaa ttgccggcag gctgcgcgcc gccatggggc gtgtaaagca gggttggcgc
     6241 catatgcgcc ctgacggcat cgagcggcgc gccatcgggc ccgtaacagc ggaaccagcg
     6301 gtcctcgccg atccctgcgc gcgagatgcc ctgcacctcg gcccaatcgg cgccagcggc
     6361 gttgagcgcc gagcgcatct cgcccgcata ccacacatcg acaatcagcg gcaggcccag
     6421 ccccgccagc cagccatgaa aggccgaggg cggcaccggc cgggcaaagg cttccttcat
     6481 ccacgccgtc agcgtgtcgc ggaagcggtg gctttcgata tattgcgccg ccgcccaggc
     6541 attgcccacc gcccggcgcg gcagggtggt cttgctggcg aagaagccgg cgaggtcctc
     6601 gggcgtcatc ggcgcgggcg gcgcccccag cgccgagatc cccggcccga gatagggcac
     6661 cacctgcccc gccttcagcc cttctgccac ttcggccagc agggcgcggg ggttttccag
     6721 cgtcagtccg ctcatctcag ccctccccat cctggcgctt gcgcgcttcc acggtgatcg
     6781 gcaggcgggt gtcctcggcc atctcgggca gttccagcac ccagccattg gcgaggcgca
     6841 cccagccgcc ccagagggcc gcgcgttctg tctcgacgat cggctcctca aggtccttct
     6901 tgggcacata ggcggatagc gcgccttccg atctgcggat catcactttc atcgctcttg
     6961 ctccgtttca ggcccggcat ggccggcggc accggcactg gccgcatcag tatcgatggc
     7021 gggcagcagc cgcagggccg gccggcggca cagcgccgcc tccagccgcc cggccagcgc
     7081 gggcaccgcg gcaatgcgcg cgcgggccag cgcacgcacc atctcctcgg ggtcgtccac
     7141 cagcgcgaac aggtcttcct ccaccgcccg ctcggccacg cagaaacgca ccgcaaggtc
     7201 gctgtcctgc gccaggatca ccagctggtc ggcgggcagg cgttctgcca cctggcggcg
     7261 cacctgcgaa tcggggtcga aggccatgtc gggcagcgcg cggatatcga tgcggcgggc
     7321 cacccactgg cgcacttccg gatcgggatc gtggcgcacc agcggcagca gctccaccgg
     7381 catgcggcgc accgcggtca ggcgcaccat ataggcgctg tcgtggatgg ccgacagcag
     7441 cgccggaccg ctcagccgcg cggccgccgc tatgcgcaca tccatgtcct catccctgat
     7501 catctcgcgg atgcgctcct cgggcaggcg cagcgtggcc atcgcccgca cctcgggttc
     7561 agggtctcgc agcagggcgg gcaggcggaa ggggttgcaa tagcgcgccg cccctgcccg
     7621 cacctcgaaa tagggatggt cgagatagct gtcggccaga tcggggttgc cgcgcaggaa
     7681 gcgctccacc cgccgcgcgc gccgatcgcg gatgcaggcc ttgcccctgg cgcacagccc
     7741 catctggttg atggcgatat gcgcgcattc gcggcagcgc accggccggc cctgccagtc
     7801 gagcgggggg ccaaggccat cctcgcccgc ttcggccgcc gcgcccatgc cccgccgccc
     7861 ggctcaggcc ggcacgcagg tgttgggcac cgggcacacc acattgcact gggcctcatc
     7921 gaaatggccc tcgcattcgg tgcatttggc cggatcgatg atgaacacat cgcccttcat
     7981 cctgatcgcg gcgttggggc attcgaattc gcaggcgctg cagccggtgc attgcgaggc
     8041 gatgatcttg taggccatgg cggatccttt ggctgtgccg ggtctgtgcc cgctgcgttg
     8101 cagaaaccgt gccagcggcg gcaaaccggg ctttgcggcc ggcggccagg gcgcgattgt
     8161 ctggtctgcg acaagggctt gtcgcagggc gcacatggat atcggtgggg gctgggatgc
     8221 cggcggggct ttagggcgcc cgcccgcagg cggcatcata ggcggcttgc gcagccacca
     8281 cggcgcccat gcgggtttcg gcccaatcga tcatcagccg cagcgccgcc agcaattcgc
     8341 ggcccagcgg ggtgatgcga taggtcacgc tcaccggcgt tgtcggcagc acttcgcggg
     8401 ccagcaggcc atcgcgttcc agcgcgcgca gggtctggct cagcatcttt tgcgacacgc
     8461 cgccaatgcg ccgcttcagc ccgttgaagc gcatcgcccc ctcgcccagc agcaagagga
     8521 tcagcacgct ccatttgtcg cccacccggt cgagcagggc gcgggtgggg caatcgcgcg
     8581 cataagcatc gccgcggcct gcatctctgc ggggggcggt tacctcgggg taatctggtg
     8641 agggaaaagt gccgtcttgc atggctggtc actatagcta accaggtcac tattggaaac
     8701 cacaaggagc atgcaagatg aagatcgccg ttctgggcgc cagcggccgc gccggatccg
     8761 agatcactcg cgagctggcc gcgcgcggcc accaggtgct ggccatcgcc cgcaagcccg
     8821 aggcgattgc cgcgctgccc ggcgttgtgg cgcaggcggg cgatgccagc gatccggccg
     8881 cgctcgccgc cctcatccag ggctacgacg cggtgatcag cgccctgcat ttcgacgtca
     8941 gcgccgccac cctgctggcc gcgctgaagc aggccggcgt ggggcgcctg ctggtcaccg
     9001 gcggcgcggc cagcctggaa gtggcgccgg gcgtgcgggt gatcgacacg cccgatttcc
     9061 ccgcggcctg gaagacggca gcgctgggcg gcatcgcctt tctcgacagc ctgcgcggcg
     9121 agagcgccat cgactggacc ttcttctcgc ccgccgcgct gatcttcgag gggccgcgcc
     9181 tgggccatta tcgccccggc accgaccagc tggttaccga cgaggcgggc gaaagccgca
     9241 tcagctttgc cgattacgcc atcgccatgg tggacgagct ggaacagcac cgccacagcc
     9301 gcgcgcgctt cactgccgcc tattaacccc ttcgtcatca atatgacatt gccatgcgcc
     9361 agattttcgc cagcaatccg gggagaatca gggtcatggc aacaattgcg atacaagggg
     9421 cgccggcgcg ccccgatatg gacagcgggc ttggcgatgt cgggctctat ggttttattg
     9481 cggttctgct ggccgcgctg ggcttcgtgg cctacagcat ctatgccgat gcggtggccg
     9541 cggggcttca gacgatggcg ctgctgccgt ttcttctgct gttcgtcgcc ctgctgatcg
     9601 cgctgggctt cgaattcgtc aacggcttcc acgataccgc caatgccgtg gccacggtga
     9661 tctacaccca ttcgctgccg gcccatgtgg cggtggtgtg gtcgggcttc ttcaatttcc
     9721 tgggggtgat ggcctccacc ggcgcggtgg ctttcggcat cgtctcgttg ctgccggtgg
     9781 agctgatcct gcaggtgggt tccaacgccg gctttgccat ggtgtttgcc ctgctgatcg
     9841 cggcgatcat ctggaacctg ggcacctggt atctgggcct gccctcttcc tcctcgcaca
     9901 cgctgatcgg ctcgatcatt ggcgtgggcg tggccaatgc gatcatgcac gggcgcgccg
     9961 gcaccgccgg ggtggactgg accaaggcca ccgaaatcgg ccaggcgctg ctgttcagcc
    10021 cgctgatcgg ctttggcgcg gcgctgctgc tgttcattgc cgccaaggcg ctgatccggg
    10081 tgaaggcgct ttatcaggaa ccccagggcg acatgccgcc gccgctgtgg atccgcgggc
    10141 tgctggtgct cacctgcacc ggcgtcagct ttgcccatgg ctcgaacgac gggcagaagg
    10201 gcatgggcct catcatgctg atcctgatcg gcacggtgcc caccgcctat gcgctgaacc
    10261 gcgccgttcc ggcccattat gaagcgcagt tccacatcgc cagcggccag gccgaggccg
    10321 cccttgccgc ccatgccggc ggcgtggctg cccctgccga catccgcgcc gaagtgacgc
    10381 attacatcgc cgacaagaag ctctcgcccg ccacggtgcc ggcgctgggc gcgctggtgg
    10441 ccgatatcga ccggcaggtg ggcgcctatc acagcctggc caaggtgccc gccgcggcca
    10501 ccggcaacat gcgcaacgac atgtatctgg ccgcctccgc gatcaagcgg ctgggcaagg
    10561 aaaaggccgt ggcgttgagc gctgccgaag ccaagacgct ggacggctac aagaaggacc
    10621 agctcgacaa ggccacgcag ttcatcccca cctgggtgaa ggtggcggtg gccattgccc
    10681 tggggctggg cacgatgatc ggctggaagc ggatcgtcgt caccgtcggt gagaagatcg
    10741 gcaagacgca cctcacctat gcccagggcg ccgtggccga gatggtgaca atggccacca
    10801 tcagcgcggc cgacaatttc ggcctgcctg tctccaccac ccatgtgctg tcctcgggcg
    10861 tggcgggcac gatggcgggc aatggatcgg gcctgcagct gggcacgctg cgcaatctgc
    10921 tgattgcctg ggtgctgacg ctgccggtct ccatcctgct gtcgggcggg ctttacgcgc
    10981 tgctgtcgct ggtggtctga tccaccaacc ttttgatcga aaccgaaggg gcgctgcgag
    11041 gcgccccttt ttttgcgctt tttcccgccc cttcctgcgt aggccaaacc gctgctggtg
    11101 gattgacttg ttgataccaa gttgcaatag cgaatcaata ttgcgactta ctcgcaaaag
    11161 aaggggtaca catggaagag attgccacgc gcagcccgca ttccgggtct atcgcgcttg
    11221 cccttggctg ccttgggctg ttcgctccgg cctttgccgc cgaagcccgg gccgcagacg
    11281 ccgatcaacc gcccgccggc accgatgccg gctccaatct gggcggcgtt accgtgaccg
    11341 acaccgccat cgacgagggc agctatcgca ccgagcggct ggccagcccc aagagcacgg
    11401 cagcgctggt cgatacgccg cgctcgatca ccgtcatctc gcaccagctg ctggaagaca
    11461 ccaattccac cacgctttca gaagcgctgc gcaccgtgcc gggcatcacg ctgggcgccg
    11521 gcgaaggcgg caacccgctg ggcgaccggc ccttcatccg cggcgccgac agccagaatt
    11581 cgatctatct cgatggcgtg cgcgacatgg cctcgcaaac ccgcgaggcc ttcgatgtcg
    11641 aatcgatcga ggtggtcaaa ggctccgaca gcgtcaccaa tggctcggcc aatgccggcg
    11701 gctcgatcaa catgatctcc aaggcccccg aggccaagcg cttcgtgcag ggcgatgcca
    11761 gcttcggcaa tgccgcctac aagcgcttca cgctggatct caaccagccg ctcagcgatt
    11821 tcgtcggctt ccggctcaat gccatgtatc actatcaggg cgtggccggc cgcgatgcca
    11881 cctggcagaa gcgctggggc attgcgccct cgatcaagtt cggcatgaac gggccgacga
    11941 gcctgcagct cgactggtat cacatgcatt ccaacgagct gcccgatgaa ggcatcccct
    12001 actactacac gctgagcaac cagcccaaca ccaacaacca gaccgcccct gccggcaccg
    12061 atgtgcgcgc gcgcggcgcc ttctatggcc tggccggccg cgatttccgc accaccaaca
    12121 ccgacaatgt cagcgcccgt ttcgaacatc atttcgacag cggcctgctg ctgcgcaaca
    12181 ccgcccgcta ttcccatacc acgcaggaat atctgtggac ccagcccgac gattccaagg
    12241 gcaatgtggc cagctacggc actgtcagcc gccgcgccaa ttcgcgctac agctacgaga
    12301 acggcatcgt cgatcaggtg gatctgtcgg gcaagttcgc caccggcgcg ctgaagcaca
    12361 gctttgccgc agcgctggag tataactggc agaaatcggc ctatggcagc tattatgcca
    12421 atggcaccac cggcgccgcc ctgcccacgg ccagcacctg cctcaccgcc acatcgaacg
    12481 gcaacgccaa ttgcacgagc cttgccaacc ccaaccccta tgatgcctat tcgggcacct
    12541 tgcagaaggg gccggccaat tcgatgacac tggccgacag ctccactctc tcggcctcgc
    12601 tgttcgacac gatcacgctg aacgacaagt ggatcctcac ccttggcggc cgcttcgacc
    12661 attacgtaac ccacgccagc gccggcctca gcgccaccac ctatcccaat ttcgttggca
    12721 cccgcacctg gctcgcgaag acggacgacc tgttcaccta tcaggccggc cttgcctaca
    12781 agccgcgccg caacggcacg atctacatct ccacctccac cgccgccatt ccgccgggct
    12841 cgttcctggc ccagggcagc gaggacaaca gcctgaccac caccaccggc atcaccgtca
    12901 acgacctcaa ggtgcagaag accacatcct atgaagtggg caccaagtgg gatctgttcc
    12961 acaacgccct ggcgctcagc ttcgacctgt tccagacccg caccaccaat gcgcgcacca
    13021 ctgccggcga tggcacctcg gtggcctttg tcgggctgaa gcgggtgcgc ggcatcgagc
    13081 tgggcctcag cggcaacatc accccggcgt ggagcgtgtt tggcggctac acctacatgg
    13141 attccatcgt gctggatgcc ggcaccacca agacaaccac cggcacctat gttgcctatg
    13201 cgccggccgc ggccaccggc aagcccttcc ccaacacgcc caagaacagc ttcaccctgt
    13261 tcaccaccta caaggccacc caccgcctca cgctgggcgg cggcgccatc tacatgagca
    13321 aggtctatgg cggcttttcc gacagccgcc aggccaatgc cgatggcacc gttaccatcc
    13381 tgaagacgcg cgccacctat gtgccggcct attggcgctt cgatgccaat gccagctaca
    13441 agctgaacga cttcctgacg ctgaagctga gcgcgctgaa ccttaccaac aagctctact
    13501 acgaccaggc ctatgccacc cattatgcgc atcaggccgc cgggcgcacg gtgatcggca
    13561 ccgtcagcgt gaaatactga tgagcgattg tccgcataac gccgggcccg tgtcggcgca
    13621 ggggggggcc tccctccccc tgcccgcagg ggtgccggcc gatgccgaag cctggcgcgc
    13681 cctgctttcc gggcccgccg aggcggccgc acaggcgctc tatcaggccg cccttgccgg
    13741 cgatgccgaa gcccagcttc tctacggcca gcacctgctc gacggggccg gcgtggtatg
    13801 cgatccggcc gcggccctgg gctggtttca tgccgcggcg caggccggcc tgccggccgc
    13861 catcaacatg gtcggccgct gcctcgatca gggctggggc acgccgccag acccggccgc
    13921 cgctgccgcc tggttcgagg ccgccgccgc ccgcgggctg gactgggggc tctacaacct
    13981 tgccaccgcg ctggcgctgg gccgcggcct ggcgcaggac cggccccgcg cgctggcgct
    14041 gttccgccgc gcggccgcca tgggccatgc caaatcgatg aacatggtcg gcagcttcca
    14101 cgaggatggc tggagcgtgc cgcccagccg cctgctggcc gcctatcact atgcccgcgc
    14161 ggccgagggc ggcgattttc gcggtgcctt caaccacgcc cgcctgctga tcgaggagga
    14221 ccagagcgag gccgccctgc cctggctcaa ccgcgcccac gccgccggca ccgcccgctt
    14281 tgccggccag atggccgcct ggctggccgc ccggccagag cccgccctgc gccaggccgc
    14341 cgcccgctgg caagccgaaa ccccggccga aagccacccc cgtgccgcct gaagccacca
    14401 aatcctcccg ccgaccgcgc agcgcccgcc agttctggat gcgccagctg cacagctggc
    14461 actggatcag cgcggcgctt tcgctggtgg gcatgctgct gttcgcgctc accggcctca
    14521 cgctcaacca tgccgatgcc atccccgccc atccgcaggt gacagaccgc gctggccagc
    14581 tgcccgccag cctgctgccg ctgctggccc acccggccag cgccgatgcg ccgctgccgg
    14641 cggccgtggc cgccgccgct gccgaggcga ccggcctcaa ccccgccggc cacccggccg
    14701 aatggtctga cgatgccgcc tatgtggccc tgccgcgccc gggcggcgat gcctgggtga
    14761 gcattgcccg caaggatggc cagatcacct ccgaagtcac ccggcgcggc tggatctcgc
    14821 tgctgaacga cctgcacaag ggccgcaatg ccgggcccgc ctggggctgg ttcatcgatc
    14881 tgttcgcgct ggcctgcgtg atcttcaccg gcaccggcct gctgctgctg caactgcatg
    14941 cccgccaccg ccccagcacc tggccgatcg ttggcgcagg gctgctgatc ccggtgctca
    15001 tcgcccattt cctcgttccc tgatcccccc tcggagtatc cccccgcatg cgcaatccct
    15061 cgcttctggc gccgctggcc gccggcctgg tcgctactcc ggcgctggcc ggcaccgttt
    15121 cggtgcagat cccccgcgtc gatgtcgccg aatatcaccg cccctatgtc gccatctggc
    15181 tggagccggc cgcagggggc agcccccgca cgctggcggt gtggtatgat gccaagcacc
    15241 ggggcgagcc gggcaccaaa tggctgtcgg aactgcgcgg ctggtggcgc gtggccggcc
    15301 gcgccatggc cgtgctccct gaagggctga gcggcgccac ccgcgcgccc ggccaatatg
    15361 ccatcccgct gcctgcagac ctgaagcccg gcgcctacac gctgaaggtg gaagccgcgc
    15421 gcgagaccgg cgggcgcgag ctggtgagcg tgccgatcac cctgccgcgc ggcggcgggc
    15481 gcgcggcagg ccatggcgag ctgggcgctg tcgccgtcgc cctgccctga aaccctgagg
    15541 ccctgaagcc ctgaagctga tccccctttc ccccaaggag aatgccgatg tcctcttccc
    15601 gtttgcgcct gctgctggct ggcgcgctgc tggctggccc catgctagcc cctgccgcag
    15661 cctttgccca ccgcccctgg ctgctgcctt cggccaccag cttttccggt gaggatccgt
    15721 gggtctcggt cgatgcggcg gtatcgaacg acctgttctt tgccgatcac ttcccgatgc
    15781 aaaccgccca gatccacgtg gaagcgcccg atggcgcggc cggccagatc gagaatgcgg
    15841 ccaccgggcg ctatcgctcc accttcgatg tgcacctcac ccgccccggc acctggaaga
    15901 tcggcaccga cagcacgatg ctcaccggca gcttcgtggc cgatggcgtg gcgcatcgcg
    15961 ttggccgccg cccgggcggc cctgctggcc ccggcggccc cggtggtccc ggtggcccgg
    16021 gcggggaacg ccccggtggc ggcaagcccg aacagggcaa tggccccgcc tttgccggcc
    16081 cgcccgtggc ggcagaagac atccccgcca atgccaccga catcaagctg gtcgaggcga
    16141 tcagccgcaa cgagatcttc gtcaccagcg gccagcccac caccggcgtg tttgccccca
    16201 ccggcaaggg gctggaactg tcgcccatca cccaccccga cgatctcgtc gccggcgagg
    16261 tcgccaagtt ccgcttcctg atcgacgggc gcccggccgc cggcctcaag ctcaccatca
    16321 tccccggcgg caagcgctat cgcgatgccg aggggggctt tgccgttacc accggcgccg
    16381 atggcgtggc cagcatcacc tggccatcgg ccggcatgtt ctggctgaac gcggtggcca
    16441 ccgacaacca cccggccgcc ccgcgcgcca ccgagcggcg gatgagctat acgaccacgc
    16501 tggaagtgat ggccccctga gcatggccag cctgcccgcc gccgctcctg ccgccgcccg
    16561 cattgccctg ccggcgcgga tagatgcggc agcggtgcag gccccgcccg ccgccggcac
    16621 gctccaccgg ctgcgcggcg agacgatggg cacgctgtgg cgggccacgc tggccgcgcc
    16681 cggcatggca ccggcggcgc tggaaggcct catcacccgg cgcctggccg gcattgtgga
    16741 tgaaatgagc cactggcagg cggacagctg ccttggccgc ttcaaccgcc tgccggccgg
    16801 cggctggatg gcgctgccgg ccgattttgc ccgggtgatg gccgccgccc tggcccttgc
    16861 cgaagcctct ggcggcgcgt tcagcccggc gctggggcgg ctggtggatc tgtggggctt
    16921 tggtccgccg gggccggtgc ccgccccgcc ggcagcccag ccgttggccc gcgcccatgc
    16981 cgatgccgac tggcgccggc tggcctttga gcccgcccct gcccgcctgc gtcaaccggg
    17041 cagactggcg ctggatctct ccgccattgc caagggcttt gccgtggatg cgctggccga
    17101 ggcgctggtg ggcgccggca tcgcccattt cgccgtggaa gtgggcggcg aatggcgcgg
    17161 cagcggcgtg cagccgggcg ggcagccgtg gtgggtggat ctggaaacgc cgccgggcgc
    17221 ggcgctggcg cccttgcgca tcgcgttgca cggcctgtcg gtggccacct ccggcaccta
    17281 tctgcgcggc gatcacaacc tcgatccgcg cagcgggcgg ccggcggcgg gcggcatcgt
    17341 ttcctgcagc gtggtgcatg ccagcgccat gatcgccgat ggctgggcca gcgcgctgac
    17401 ggtgctgggc cctgaggccg ggctggagat ggccaaccgc cagggcctgg ccgcgcggct
    17461 ggtgctgcgg ggaggcgccg aggtgatcag cacgccgctc cgcgccctgc tggccgatta
    17521 gcgcggcggc ggggccccat gcgctttcgg ttgacaaatt caataatgac tcgcaatagc
    17581 gtggcgagca cgccgtatcg tcatcggggg agatgtccca ttttggccga gagtaccgcc
    17641 cttctgcgct tcgtcgacac cccgctggcc gcattgggcg cccctgggcc ccagcgccgc
    17701 gccccggcgg cccgtgcgcc ccaggccccg ttcctgcgca ccgcaccctc ctatgcggcg
    17761 ccccggccgc gccagcgcct ggccgccgcg atcgtctcgg ccggcacggt ggcggcgctg
    17821 ttctcggcca tcgcggtgat gaacgccatg catgtggccc accgcgaacc gcgcatcgtc
    17881 gccatcaacc tgaccccgcc gatggcgccg ccgcagcccc ccaaggccaa gcccgcgccc
    17941 acccccaggg aagccgtggc ccagcccgcc acgccggcgc ccgcccagcc ggccgccacc
    18001 gcagcgccgg cgccgatcgc ggcggccctg cccgtcaaca gcgcgccgcc tgcgcccccc
    18061 gcgccggccg cagcgccggc accggcgccc gcccctgccg cggcgcccgc cgcccccgcc
    18121 atggccgagg cgggcgatct gtcggcccgc atgctctcct tcgtggcgcc cacctatccc
    18181 atcgattcgc gccgggcgca tgaggagggc accgtcgtcc tctcgctgct gctgggcacc
    18241 gatggccgcg tgcaggagat ccaggtggcc cacaccagcg gctccccccg gctggatagc
    18301 gccgcccttg ccgccgtgcg caaatggcgc tgggcgccag tggtgcgcga tggccagccg
    18361 gtgctggtgc gcggcacggt gcgcattccc ttcctgctga agcgctgacc ggccctctca
    18421 ggcctcgatg gcgccgcgct gccagcgcgc gccttccagc gcataatgca gccgccggtt
    18481 gagcgcgccc gggcagcctt cccagaaggt caccgcatcg ggcagcaggc ggaaccattt
    18541 ccagtagtct ggccgggcga tgggcgcatc gctttccagc agggcgtgga aggcggccag
    18601 ctgcgcgccc tcgtccacca gcggacgacc gtggaaggag accgtgcgcg cggccatcac
    18661 cgccctgtct tcctcggcaa agcggcggtc gcattccgcc tcgccggcaa tgtcgatagt
    18721 gccggtcagg ttgatcgact gggccgtctc gcgccagtag atcgacaccg ccgcccggcc
    18781 cgtggccagc atctccagcc ccttgcggct gccgaaattg gtggtgaaca acagcgcgcc
    18841 ggcctccacc tccagcatct gcaccgtgcg cgaggaaggc acccccgcca ccgaactggt
    18901 ggcgaaggtg acataggccg gctgctccgc gccgatcgcc agcacatgat cataccaggc
    18961 gcgcaccagc gccagcggat gatcaggcgc gccggccgca tccagccgcc cggcatgagc
    19021 gggcgtttgc ggaaacacca cctgcgacat cagaagctgt aaccgatggt gccgatgatc
    19081 tggcgcggcg ggatatattg ctggctggtc agcccctgct cgtagggcga ggtgtagcgc
    19141 gagctgaccc cggccacatc ggtggcattg ctgaccgaca gcgccagatg gaagcggctg
    19201 tggggcggct cgtaggccag gctgaggttg atgagattat aggccggctc gtgatcgaga
    19261 taggcctcgt tgaagatccg cgcctgccgc gcgccgacat ggacatactg gatccacggc
    19321 gtcaacttgc cgcccatgaa cgcaatatca taagacacat ccagcgaggc ggaaatctcg
    19381 ggcatgttgg gcggcgtgtt gccatagata tccttggcag aagcaatcac ccctgcccag
    19441 cagctgctgg aataatactg gccgccatag gcgcaggcgc ccacgctgct gtaggcattg
    19501 ttgacgatgg tgggatagag cacgttggta tggctgacca ccttgccctg ctcgacggtg
    19561 aggttaccgt tgatgtgcag ccgccctgcc atgccggtat agctggcctc tgcctcgccg
    19621 ccccagatat gcaccagcgg aatgttcgac atgccctgat cgaaggggat cggatcggtc
    19681 tcgatatatt gcatgttctt gtagatgtag aagaagcccg ccatgttgag gcgcagcttc
    19741 ttgtcgaaga agaagttctt cgagccgatc tcgaaggcgg tgttgtgctc gtccttgaag
    19801 gtgtagggca ccagcgcggt gccggcattg ccattggcac cgcccgcctt gtaaccggtg
    19861 gcagcgctga catagaccat gttctgcggg ctcagcgtat aggccagctc ggcgcgccag
    19921 gtgggcacgc tggtctcgtc cttcaggcca aacagcggat cattgccata ttcggagaaa
    19981 ttatggctga ggtgatcgtg cgattcgtaa ttgtagcgcg cgccggcggt cagcttcagc
    20041 ttgtcggtga caggatagct gaactgcacg aaaggctcca ccaccagccg cacgtcgcgc
    20101 gtccagttgc cataggacag gttgctggga atatcggccg agggcgtgga cggatcgaca
    20161 taggcgggcg tcgcgtcgct ggcattggtg ccgtaatatt ccagcgtgta atccttggcc
    20221 acgcggcgca tgaagaacac gcccgccgtc cagctcagct tcgatccggg tttggagatg
    20281 aagtcgatct cttccgaggc cgattcctca ttgtgattcc acgtcggcac atattcatag
    20341 gcgccatagc ggtcgaaggt ggtgaaggtc acattctcgg caaactcgct cttgtaggag
    20401 cgataggccg agaccgagcg gatcgagaaa accggcagat cccagttcag gttgagatgc
    20461 accatcgtcg acttgtagta gttcttcgac aggtaatcct gcgaaagcac ccgcggatcg
    20521 ggattggggt cgttcagcca cttctgcgcc gggccatgat cgtgctggcg ggtccactcg
    20581 ccagtcaacg tggccgaaaa agtgctgctg ggctgccaca gcaccgcgcc cttcactgcg
    20641 gtatcatcgg catcgtcgag gtcatagccg gggttcaccg tgttggtggt aaaaccgtca
    20701 tggctcagcc gctggcccga caggcgcacc gccaccgtgt cgctcagcgg cacattgatg
    20761 gcgccgcgcg cgcggatgta gttgtaatcg cccaggctca catcgaaatt gccgctgatc
    20821 ttgtcgagct tgggctggtt gctcaccagc agcagcgcgc cgccgatcgc cgagacgcca
    20881 taaagcgtgc cctgcggccc gcgcatcact tccatatgat cgagatcgaa caggctgtgg
    20941 cccagcgaga cgctgtcgcc cacataaacg ccatcgatga acatcgccac gcccggcgcg
    21001 gtggagccgg ggttttccgg cgtgcccgaa ccgatgccgc gaatgctggg cacggtttcg
    21061 cgcccgcccg tcttgcgcac ggtaaggccc ggggccatgc cgctgaggtc cgtcacatcc
    21121 tgcacattgg cgctgtcgag gatgttcgag ggcagcacgg tgatcgccag cgcggtggat
    21181 tgcagcttgc tgctgcgccg ctcggcggtg acgagaatgt cgccctgggc cgcgggcgct
    21241 tcgcttgccc ccgcgttatc cgcccccgct tcatccgctg ccgcctcatg cgccgctgcc
    21301 tcgcccgcgc gggcctgggc agccgtgccc aggcaccctg cccacaatgc cgcgatcacc
    21361 gctgattcgc cgaggcgcca gcgcccctta tgcctgattg ctctcatgtg cgtgccctca
    21421 tgtctggttc ccgtcatcgc gggatgcccg tgaatgggtg cgtttccacc ctgttatgtt
    21481 cgattttggt cgttttttgt tattacgaac atgagtgaac catgaaatcc ccacattggc
    21541 aagtgtgcat tgcggaaaaa cacgtcccgc aatgcccctg cagtttacgt atcgccaaaa
    21601 ccatattcag ggatgccctg gccggaccca taatatttga agggcaggaa cttgcccgaa
    21661 agggtgaatt tcacccggtc tcccttgggg tggggcaagc gctccatctc catgtcgaaa
    21721 tcgatggccg acatgatgcc atcgccaaat tcctcctcga tcaaggcttt ccatgccggt
    21781 ccattgacca tcaccagctc gtagaagcga tagatcagcg gatcggtggg cggcatcggg
    21841 ctgccgcgat ggggcacttc gttcagcatc cgctcttcct gcccatccag cccgaacagg
    21901 cgcgccgcat tggcggccag cggcttgggc agcttcatct gccccaacag tgcgcccacg
    21961 atcaaaaccg gcgaaagtcc gccaatttca tcgcagatat ggcgccagct ccagccctcc
    22021 tgccgcttgc gatcgaggat cttctccacc agctgttccc gcttcatgct cttgctccct
    22081 tgtgggccgg cagcaggcgc gctgcaggcg cgaaatatgc gtgataggtt caaaacagtg
    22141 tatggttgaa aaagaacatt gcaagccata agctggccgc cccagccgaa aggaccgatg
    22201 catgccagcc attccccagc ccccgctgcg cgatcacggg cgctttgcct acagccccat
    22261 ccacgcccgc cccgcctacg actggcagcg ccccgaaggc ggcagcgcgc ggctggccgt
    22321 ctacatcggt ttcaacatcg aacatttcgc ctttggcgag gggctgggcg cccggttggg
    22381 cgcctttgcc ggcgagcccg acgtgctcaa ctacagctgg cgcgaatatg gcaaccgggt
    22441 gggcgcctgg cgctgcctcg acctgttcga cgcgctgggc ctgcccaccg gctgcctgat
    22501 caacaccgcg ctctacgacc attgccccga actggtggcc gccttcgccc gccgcggcga
    22561 cgagctgatc ggccacggcc acagcaacgc tgcccgccag agcgacctgc ccgaagccga
    22621 ggaacgcgcc ctgctggccg cctgccgcgc ccgcatcgcc agccacagcg gcaccgcgcc
    22681 cgatggctgg ctttccccct ggatcgccga aacccacgcc acgcccgatc tgctggccga
    22741 aaccggctat cgctacacgc tgaactgggc gcatgacgac cagcccacgc gcctgcgcac
    22801 ccgcggcggc gaggggctct ggtcgatccc ctacccgcag gagctgaacg acattccgat
    22861 gatcatcgcc cgccagctgg atggccaggc ctttgcccgc atgatcatcg acaacttcct
    22921 ggaaatgcgc gagcaggcca gccagcagcc gctggtgatg ggcattgccc tgcatcccta
    22981 tctggtgggc cagccctatc gcctgcgcca tttgcgcggc gcgctggaag agatctgccg
    23041 cgcgcgcgat gccggcgatg tgtggctggc cacccccggc gccatctgcc gccagatgga
    23101 agcgctggcc gccgaggggc gtgtctagcg gcgcaccacc agccgctcgg ccagatcgac
    23161 cagcccgatc agcgccagcg acagcgccgc cgaacagaag atcgccgccc acatctgcac
    23221 caggttcagc caggccgtgg ccagcacgat ggccgcgcca atgccgtctg ccgtgccggt
    23281 gaattcggag acgatggcgc ccaccaccgc cgcgacgatc gccagccgcc cgccggcaaa
    23341 cagatgcggc aggctggacg gcaggcgcag ccgcaggaaa atgtcgagcc ggctggccgc
    23401 cagcgcgcga aacacttcca gcgcttctgc atcgatcgcc tgcagcccgg ccatcgcgtt
    23461 gatcagcatg gggaaaaacg ccgcaatcgc ggccacgata acgtgcggcg tggcattgaa
    23521 gccaaacacc acgatcagcg ccggggcaat cgccacgatc ggcgtgacat tgagcagcag
    23581 cgccaccgga aagatcgcat ccctgaggaa gcggaactgc acgaagcaga tcgccagcag
    23641 caccgccgcc aacccgccca gcaccagccc cgccagcgcc acgctggccg ttgtccacac
    23701 attggcggca tagaaagccg ggcgcgcggc cagatcgccc gccacatcca gcagcgaggg
    23761 caagatcgcc ccgtgccgcc ccgccaccca ctgccaggcc agccccagca gcagaaaggt
    23821 aatggccggc gccagcaggc ggcgcgcagg ctgggcgatc atggccgttc tccttcgcca
    23881 aggcctgccc acaaggcgtg cttcacctgc gccaccagtg cggcaaattc ggccgtctcg
    23941 gtcatttccc agcggcgcgg gcggggcagg tcgatggcga tatcggcatg cactcggccc
    24001 ggccccgggc tcatcaccag cacccggtcc gacagcagca ccgcctcgaa agcggaatgg
    24061 gtaacgaaca gcaccgtctt gcggtgcttc tcccacacct ccagcaagcg ccagcacaag
    24121 gcgttgcggg tcagctcgtc gagcgcggag aaaggctcgt ccatgaacat tagcggcgcg
    24181 cccaccacaa
//
LOCUS       DASRYE010000274         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_260244,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000274 DASRYE010000000
VERSION     DASRYE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260244"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFF98_14110"
     CDS             <1..592
                     /locus_tag="VFF98_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZU64814.1"
                     /translation="PPRLFRVSFTGELGFEINVPAEHGRAVWEAIWKAGQPYAITPYG
                     TETMHVLRAEKGYIIVGQDTDGTLTPYDAGLAWAVGAKKADFVGKRSLARNDLVRKGR
                     KQLVGLLTENPTTVLEEGAQIVLDPKAPVPMPMVGHVTSSYWSANLGRSIAMGVVADG
                     HAKMGDTVFIPMPEGTLRATITSTVFIDPEGSRLNG"
     gene            585..1112
                     /locus_tag="VFF98_14115"
     CDS             585..1112
                     /locus_tag="VFF98_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase gamma subunit"
                     /protein_id="HZU64815.1"
                     /translation="MAETITIAPLAIGAEDLALGAGPQVLAGAAIAQAPAATRWSLRA
                     RDPDVLAGLIGRALPQKIGETLHGIAKLGPDEWYALLPADEALPDGAGLPVCVVDVSS
                     RAIGIRVEGQGAADIIGAGCPLDIAAIAPGRATRTVFETVEIVLWRESETRFHIEVWR
                     SFAPWLWNALIASAG"
     gene            complement(1187..2143)
                     /locus_tag="VFF98_14120"
     CDS             complement(1187..2143)
                     /locus_tag="VFF98_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU64816.1"
                     /translation="MRLPPEFDLHALEVFVLTVELGGMTASAQQLRVTQSAVSQTITR
                     LESGMGTPLFDRALRPLGLTPAGRALYQRAKRLLATARTMYDEVREGAQQPIDQIIIG
                     MSESLATLLTAPLLRRHGNRVGRWSIRAGISLNQQQDFLQRRYDMLVTGSNTLEKHEG
                     VEHHRVMDDPFLMVFPASYEGSTDPGEIKDRLPFVRYSLDTGMGQRIESQLARMKLRL
                     PNVIEVEIINQQLTAVAMGIGWSITSLLCLAALPGLLPQLRVEPLPRGRFSRSIDVVA
                     RSDELADLAEQTALLARESLEHDALPPLLAAYPWAGSMLGWD"
     gene            2273..3133
                     /gene="purU"
                     /locus_tag="VFF98_14125"
     CDS             2273..3133
                     /gene="purU"
                     /locus_tag="VFF98_14125"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZU64817.1"
                     /translation="MALIKPDFVLLVSCVDRKGIVAAIANSIAAQDCNIVENSQFGDA
                     ETGRFFMRVAIAAPKSVSVDSFKAAFLPVASAYHLDWQVHDLHVKQRAIIMVSKGGHC
                     LNDLIYRTATGFLPMEITSIVSNHMTWARRAEHEGLPYHYLPVTPETKAAQEEKLMAL
                     IDEEQVDLIILARYMQVLSDATCRKLEGRVINIHHSSLPAFKGARPYHRAYERGVKLV
                     GATGHYVTPDLDEGPIIAQDVTHVDHADTVDDLIAQGQETESRVLTRAVKAHLEHRVM
                     LNGQRTIVFK"
     gene            3159..3659
                     /locus_tag="VFF98_14130"
     CDS             3159..3659
                     /locus_tag="VFF98_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HZU64818.1"
                     /translation="MSEHRLYADLEGLGIAWQALEHEAVFTVEDSAAIHAALPGAHTK
                     NLFLKDAGGQFWLVTVDHEKRVDLKATAGAIGAKKLSFGKAEDMAALLGVAPGAVTPL
                     AAINDGAGAVQVVLDTALAGAAQVHVHPLRNTATIGLAGADLCRALAHWNHAPLIATI
                     PERAPA"
     gene            3670..5046
                     /locus_tag="VFF98_14135"
     CDS             3670..5046
                     /locus_tag="VFF98_14135"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZU64819.1"
                     /translation="MSVSAFDLFKIGIGPSSSHTVGPMVAARRFGEALEGEGLLAAVA
                     RLEVDLYGSLALTGKGHASDVAVILGLAGHLPATVEPDAVPAILADVAARGRIAFMGR
                     HDVAFDPVADIHFLMRERQPFHSNAMAIAAYDAAGQQIARRFYYSVGGGFVLDEEEAL
                     RNAPAADEVPVPYAYASAAELMELGEASGLTICQMMLANELAHRTRAEVEAGLDAIAD
                     TMAACIDRGCAQDGVLPGGLKVKRRAARLASELAARQQMALNEPLAILDWVNLWAMAV
                     NEENAAGGRVVTAPTNGAAGIIPAVLRYYERCYRGTREGRRSFLLAAAAIGSLYKRNA
                     SISGAEVGCQGEVGVACSMAAAGLTAALGGTNAQVENAAEIAMEHNLGLTCDPIGGLV
                     QIPCIERNAMGAVKAIDASRLAMLGDGSHVVSLDKVIEVMRQTGADMRDKYKETSQGG
                     LAVNVVEC"
     gene            5066..>5203
                     /locus_tag="VFF98_14140"
     CDS             5066..>5203
                     /locus_tag="VFF98_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZU64820.1"
                     /translation="MKILVPVKRVIDYNVKPRVKADGTGVDLSNVKMSMNPFDEIAVE
                     EG"
BASE COUNT          864 a         1691 c         1779 g          869 t
ORIGIN      
        1 ccccccccgc ctgttccgcg tgtcgttcac cggcgagctg ggctttgaaa tcaacgtgcc
       61 ggccgaacat ggccgcgccg tgtgggaagc gatctggaag gcgggccagc cttatgcgat
      121 caccccctat ggcacggaaa ccatgcatgt gctgcgcgcc gagaagggct acatcatcgt
      181 gggccaggat accgatggca cgctgacgcc ttacgacgcg ggcctggcct gggcggtggg
      241 cgccaagaag gcggatttcg tcggcaagcg atcgctggcg cgcaatgatc tggtgcgcaa
      301 gggccgcaag cagctggtgg gcctgctcac cgagaacccc accaccgtgc tggaggaagg
      361 cgcgcagatc gtgctggatc ccaaggcacc ggtgccgatg ccgatggttg gccatgtcac
      421 ctccagctac tggagcgcca atctgggccg ttccatcgcc atgggcgtag tggccgatgg
      481 ccatgccaag atgggcgaca cggtgttcat cccgatgccc gaaggcacgc tgcgtgccac
      541 catcacttcc actgtcttca tcgatcccga agggagccgc ctcaatggct gagaccatca
      601 ccatcgcgcc gctggccatt ggcgcggaag atctggcgct gggggcaggg ccgcaagtgc
      661 tggcgggcgc tgccattgcc caggcgccgg cggccacccg ctggtcgctg cgcgcgcgtg
      721 atcctgatgt gctggccggc cttatcggcc gcgccctgcc gcagaagatc ggcgagacgc
      781 tgcatggcat cgccaagctg gggccggatg aatggtatgc cctgctgccg gccgatgaag
      841 cgctgcccga tggcgcgggg ctgccggtct gcgtggtcga tgtctcctcg cgggccatcg
      901 gcatccgcgt ggaagggcag ggcgcggccg acatcatcgg cgccggctgc ccgctggata
      961 tcgccgccat tgcgccgggc cgggccaccc gcacggtgtt cgagacggtg gagatcgtgc
     1021 tgtggcgcga gagcgagacg cgctttcaca tcgaggtgtg gcgcagcttt gcgccctggc
     1081 tgtggaacgc cctgatcgcc agcgcgggct gagcctttcc cggggctggc gggaaggaag
     1141 ggggcgcggc gcggctttgg ccgtctcgcg cccccttttt gcatcctcaa tcccagccca
     1201 gcatcgatcc tgcccaggga taggccgcca gcagcggcgg cagcgcatca tgctccagcg
     1261 attcgcgggc cagcagggcg gtctgctcgg ccaagtctgc cagctcgtcg ctgcgcgcca
     1321 ctacatcgat gctgcgcgag aagcgcccgc gcggcagcgg ttccacccgc agctgcggca
     1381 acaggcccgg cagcgccgcc aggcacagca gcgaggtgat gctccagccg atgcccatcg
     1441 ccaccgccgt cagctgctgg ttgatgatct ccacctcgat cacattgggc aggcgcagct
     1501 tcatgcgcgc cagctggctt tcgatgcgct ggcccatgcc ggtatccagc gaatagcgca
     1561 cgaagggcag gcggtccttg atctcgccgg ggtctgtcga gccttcgtag ctggccggga
     1621 agaccatcag gaaggggtcg tccatcaccc ggtggtgttc cacgccctcg tgcttttcca
     1681 gcgtgttgct gccggtcacc agcatgtcgt agcggcgctg gaggaaatcc tgctgctggt
     1741 tgagcgagat ccctgcgcgg atcgaccagc gccccacgcg gttgccatgg cggcgcagca
     1801 gcggtgcggt cagcagcgtg gccaggcttt ccgacatgcc gatgatgatc tggtcgatcg
     1861 gctgctgggc gccctcgcgc acttcgtcat acatggtgcg cgcggtggcc agcaggcgct
     1921 tggcccgctg atagagcgcg cggcccgcag gcgtgaggcc cagcgggcgc agcgccctgt
     1981 cgaacagcgg cgtgcccatg ccgctttcca gccgcgtgat ggtttgcgac acggccgact
     2041 gggtaacgcg cagctgctgg gcgctggctg tcatgccgcc cagttccact gtcagaacaa
     2101 acacttccaa agcatggaga tcaaattccg ggggcaggcg caaggggcgt tctccggttc
     2161 agcgataaag ggatctgaac tatcgtatta tagatactta tcggtgcgca agggcgttcc
     2221 atctcccgcc gccatgcgta agctgggcgc atccaatcgg ggagataatc gcgtggctct
     2281 catcaagccg gatttcgtgc tgctggtatc ctgcgtcgat cgcaagggca tcgttgcggc
     2341 cattgccaat tccatcgcgg cgcaggattg caatatcgtc gagaacagcc agttcggcga
     2401 tgccgaaacc ggccgcttct tcatgcgcgt ggcgatcgcc gcgcccaaga gcgtctcggt
     2461 cgacagcttc aaggcggcct tcctgccggt ggccagcgcc taccatctcg actggcaggt
     2521 gcatgatctg catgtgaagc agcgggcgat catcatggtc tccaagggcg gccactgcct
     2581 gaacgacctg atctaccgca ccgccaccgg cttcctgccg atggagatca ccagcatcgt
     2641 ctccaaccac atgacctggg cgcgccgcgc cgagcatgag gggctgccct atcactatct
     2701 gccggtcacg cccgaaacca aggcggcgca ggaagagaag ctgatggcgc tgatcgacga
     2761 ggagcaggtc gatctcatca tcctggcgcg ctacatgcag gtgctctcgg atgccacctg
     2821 ccgcaagctg gaaggccggg tgatcaacat ccaccattcc tcgctgcccg ccttcaaggg
     2881 cgcgcgcccc tatcaccggg cctatgagcg cggcgtgaag ctggtgggcg ccaccggcca
     2941 ttacgtgacg ccggatctcg acgagggccc gatcatcgcc caggatgtga cccatgtgga
     3001 ccacgccgac acggtggacg acctcattgc ccagggccag gaaaccgaaa gccgggtgtt
     3061 gacacgcgcg gtcaaggcgc atctggaaca ccgggtgatg ctgaacggcc agcgcacgat
     3121 tgtgttcaaa taacccgccg cctgccaaag gaaatgtgat gagcgagcac cggctctatg
     3181 ccgatctgga agggctgggc attgcctggc aggcgctgga acatgaggcg gtgttcaccg
     3241 tggaagacag cgccgcgatc cacgccgcgc tgcccggtgc ccacaccaag aacctgttcc
     3301 tgaaggatgc cggcggccag ttctggctgg tcaccgtgga tcacgagaag cgggtggatc
     3361 tgaaggccac ggcaggggcc atcggcgcca agaagctctc cttcggcaag gccgaggata
     3421 tggcggcgct gctgggcgtg gcgccgggcg cggtcacgcc gctggcggcg atcaacgatg
     3481 gcgcgggcgc ggtgcaggtg gtgctggata cggcgctggc cggggccgcg caggtgcatg
     3541 tgcacccctt gcgcaacacc gccaccattg gccttgccgg cgccgatctg tgccgcgcgc
     3601 tggcccattg gaaccatgcg ccgcttattg ccaccattcc cgaacgcgcc cctgcctgag
     3661 gccctgctca tgagtgtttc cgccttcgac ctgttcaaga tcggcattgg cccgtccagc
     3721 agccacacgg tgggcccgat ggtggctgcc cgccgctttg gcgaggcgct ggagggcgag
     3781 gggctgctcg ctgccgtcgc ccggctggag gtggacctct atggctcgct ggcgctgacg
     3841 ggcaagggcc atgccagcga tgtggcggtg atcctggggc tggccggcca cctgcccgcc
     3901 acggtggagc ccgatgccgt gcccgccatc ctggccgatg tggccgcgcg cgggcgcatt
     3961 gccttcatgg gccgccacga tgtcgccttc gatccggtgg ccgacatcca cttcctgatg
     4021 cgcgagcgcc agcccttcca ttccaacgcc atggccatcg ccgcctatga tgccgccggc
     4081 cagcagatcg cccggcggtt ctattattcg gtgggcggcg gctttgtgct ggatgaggaa
     4141 gaggcgctgc gcaacgcgcc ggcggcggat gaggtgccgg tgccctatgc ctatgcctca
     4201 gccgccgagc tgatggagct gggcgaggcc agcggcctca ccatctgcca gatgatgctg
     4261 gccaacgagc tggcccaccg cacccgcgcc gaggtggaag ccgggctcga tgcgattgcc
     4321 gacacgatgg ccgcctgcat cgaccggggc tgcgcgcagg atggcgtgct gcccggtggc
     4381 ctcaaggtga agcgccgcgc cgcgcgcctc gccagcgaac tggccgcccg ccagcagatg
     4441 gcgctgaacg agccgctcgc tatcctcgac tgggtgaacc tgtgggcgat ggcggtgaac
     4501 gaggaaaacg ccgccggcgg ccgcgtcgtc accgcgccca ccaatggtgc ggcgggcatc
     4561 atcccggcgg tgctgcgcta ttacgagcgc tgctatcgcg gcacgcgtga agggcggcgc
     4621 agcttcctgc tggcggccgc tgccatcggc agcctctaca agcgcaacgc ctcgatttcc
     4681 ggcgccgaag tgggctgcca gggcgaggtg ggggtggcct gctcgatggc tgcggccggc
     4741 ctcacggcgg cgctgggcgg caccaatgcg caggtggaaa acgccgcgga aatcgccatg
     4801 gaacacaatc ttggcctcac ctgcgatccc atcggcgggc tggtgcagat cccctgcatc
     4861 gaacgcaacg cgatgggcgc ggtgaaggcc atcgatgcct cgcgccttgc catgctgggc
     4921 gatggcagcc acgttgtcag cctcgacaag gtgatcgagg tgatgcgcca gaccggcgcc
     4981 gacatgcgtg acaaatacaa ggaaacttcc cagggcggcc tcgccgtcaa cgtggtggaa
     5041 tgctgatcaa tcaagggttt gagccatgaa gatactggtg ccggtgaagc gtgtgataga
     5101 ctacaacgtg aagccgcggg tgaaggcgga cgggacgggt gtggacctgt cgaacgtgaa
     5161 gatgagcatg aacccgtttg acgagatcgc ggttgaagaa ggg
//
LOCUS       DASRYE010000275        14316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_246162,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000275 DASRYE010000000
VERSION     DASRYE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246162"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..691
                     /locus_tag="VFF98_14145"
     CDS             245..691
                     /locus_tag="VFF98_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HZU64821.1"
                     /translation="MQGTILAFDFRSGEGVISGEDGLRYRITMQEWKHALPPAVGQTV
                     DFQPAGDIAGAIYVIQQPSPFSGDKNRLVAALLALFLGMLGVHKFYLGKNTAGLIMLA
                     GGTIGWVLVLPGLAVAVIAFIEFIIYLTTSDADFAARYVNGPRAWF"
     gene            complement(731..1219)
                     /locus_tag="VFF98_14150"
     CDS             complement(731..1219)
                     /locus_tag="VFF98_14150"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZU64822.1"
                     /translation="MTSAPTIADFTARLASGEEISLADRLGKVVLVVNTASKCGFTPQ
                     YKGLEALWRRYGGQGFEVIAFPCNQFGGQEPGSAEEIAQFCAVNFGLSFPLMGKVEVN
                     GPGAPPLWQWLKREARGLFGTQRIKWNFTKFLIDREGRVIRRFAPADAPEKLAGAIEA
                     LL"
     gene            complement(1244..1525)
                     /locus_tag="VFF98_14155"
     CDS             complement(1244..1525)
                     /locus_tag="VFF98_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64823.1"
                     /translation="MSQPPSTFRGEPYQPGTIDWTETASLHRADDGGGLLDGLKALRQ
                     GALADLVRFIALLPEGERDAYAIERQSDHRLSTAEVMELYALADFPHAA"
     gene            complement(1638..2333)
                     /locus_tag="VFF98_14160"
     CDS             complement(1638..2333)
                     /locus_tag="VFF98_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU64824.1"
                     /translation="MSEAIVELAGVTRRFTQGGVALDVLRGVDLAIRPGEIVALLGPS
                     GSGKSTLLQAVGLLEGGFGGSIRIGGAEAAALDGEGRTRLRREAIGFVYQFHHLLPDF
                     TALENVVLPQMIAGTARPAAEARGQELLAALGLGQRLGHRPSQLSGGEQQRVAVARAL
                     ANRPRLVLADEPTGNLDEATADIVLGQFVELVRGLGSAALIATHNERLAAAMDRVVRL
                     HEGRLEEGARQQG"
     gene            complement(2326..3567)
                     /locus_tag="VFF98_14165"
     CDS             complement(2326..3567)
                     /locus_tag="VFF98_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZU64825.1"
                     /translation="MLLSPFEWTMARRYMLPGKGERFIALVAGISLVAVMLGVAALVI
                     VMSVMNGFRGELIDKIVGLNGHAIIQAYGGRLDDWRAVLKQTEATPGVIHASPLVEQP
                     LLASFNGRVEAILVRGNTAPDIARLKPKLAAGNLADLSPGSDNVAIGARLAEAMGARV
                     GDDITVINPVGRATPFGTVPRQVSYHVAAIFEIGVYDYDNTYVVMPIEDAQTLLLSGD
                     TIGMIEVTTSNAEKAGEILAPLAQTLSGRAVVTDWQQLNAQLFEALAVERVSMFVVLS
                     IIVLVAVFNILSSLIMLVRAKTRDIAILRTMGASSRSVLKIFVATGFVIGAAGTVAGL
                     LLGFVFLYFRQNVVNAVQFITGQNLWDPKIRFLTELPSRPDPVEIAVIAGMALLFSFL
                     ATLYPALKAASTDPVQVLRYE"
     gene            3752..5527
                     /locus_tag="VFF98_14170"
     CDS             3752..5527
                     /locus_tag="VFF98_14170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU64826.1"
                     /translation="MICSTRKRALFLPVIGYAAAACLALRFARFDGGVAYVWGANAVL
                     AAVLSTLPRGRWWEVLLPCFFAGWLTNGLWGLGWQLAPGLAFGNMADVVVAVALLRRL
                     TRTADARIPKAEPTPPLLPFVAAMGLAGPATSTLLATLTVMLSGRPPSAPLALTWFIG
                     HGLGMLAFYPGLLVIVRGTRRAGTPLAEKAPRHRAWPRQAGVLLLFTAACLYTFAQQG
                     RPLLFVPTLVLAAAIAWAETATLALAYGILALVGGAFTAMGHGPIALMAVSMPQRMQM
                     LQIYLALSVVCTLPMASIVHRRVQLLARMRESEARYRLLADNSTDIILSTDAAGRIRF
                     VSPSVLLLAHHQPAELIGRLARDFVVPEHRAQAIAAHLAAMRAPGSTITAEFLGLVRY
                     GEPRWFESRMRAVAGDDGRVECVVNVVRDMSRRKEYETALALAALTDPLTGLPNRRLF
                     RNALEDCIANAGCGCVALIDLDHFKLVNDRHGHAAGDAVLKSVAAAARAALRGSDTFA
                     RMGGEEFALLLPGASLEVAQGVCRRLASTLAATTTEHEGQAIRITCSVGLARLGRDAD
                     TAMAAADRALYDAKAAGRDCLKIAA"
     gene            5713..7200
                     /gene="purF"
                     /locus_tag="VFF98_14175"
     CDS             5713..7200
                     /gene="purF"
                     /locus_tag="VFF98_14175"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZU64827.1"
                     /translation="MNASPSPRDTTPWDIAPDGDGGDHLHEECGIFGVIGARQAASMV
                     ALGLHALQHRGQEAVGITSFDGTEFFSYRGIGHVAHVFNNQDIFADLPGGMASGHVRY
                     STTGGSGLRNIQPLFADLASGGFSIAHNGNISNAMTLKRDLVQKGSIFQSTSDTEVII
                     HLVATSRYPTLLDRFVDALRLIEGAYSLIVMTPEGMIACRDPLGIRPLVMGRIGDSVV
                     FASETVALDVVGAEFLREVEPGEMIEVDHKGVISSHRPFGTQPARPCIFEHVYFSRPD
                     SVMGGRSVYEARKAIGKELAKEAPADADLVIPVPDSGVPAAIGYAQESGIPFELGIIR
                     SHYVGRTFIQPSDGARHADVKRKHNANRALVQGKRIVLIDDSIVRGTTSLKIVQMMRD
                     AGASEVHMRIASPPTEHSCFYGVDTPERSKLLAARMDLDAMAEFIHADSLAFISIDGL
                     YRAVGEAERKPACPQFCDACFTGDYPTRLTDLVAAGKADGVNQPD"
     gene            7207..7932
                     /locus_tag="VFF98_14180"
     CDS             7207..7932
                     /locus_tag="VFF98_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU64828.1"
                     /translation="MSEETQPFAGQLALVTGASRGIGAETAKALAAAGAHVVLTARDT
                     RALEAVEEEIHRAGGHATIAPFDLAEPDAIARLAAAITERWPALDILVINAGVLPTLT
                     PVTDIDPKAFNRAIAVNVLATQALLACFTPLLRKSADARVIGLTTSVAGTPRAYWSAY
                     GATKAAQEVLLTSYAQEMRNISNIRVAIVNPGATRTAMRARAYPGEDPASVKPPEVVA
                     RRLVGLLTEPFVSPSREDIPKGW"
     gene            8013..8450
                     /locus_tag="VFF98_14185"
     CDS             8013..8450
                     /locus_tag="VFF98_14185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU64829.1"
                     /translation="MSQSQPSPLAARLSPQRRAHLRAVEERVSQLTVIQPRPAEDQHE
                     QRAAPRIRVAVPVIITLHTGQSFHSVVHDLSISGFSASAMVRMEPESRCIMKLPGLAG
                     RQARVVWWNASIVGCAFAEPLTTATLDELVERYRHAATLHPSA"
     gene            complement(8455..9834)
                     /locus_tag="VFF98_14190"
     CDS             complement(8455..9834)
                     /locus_tag="VFF98_14190"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZU64830.1"
                     /translation="MPQAPVLLWFRRDLRLADQAAVLAACASGAPVIPVYVLDDDTPR
                     HRALGGASRWWLHGSLASLAGDLAARGSRLVLRRGAVADVLPALAAEVGATTLHALHH
                     YEPWWLNAEKGLRRAGLDLQLHHGNFLLPPGSVTTGQGTPFRIYTPFWRALQLHMPPP
                     VPQAAPGAIPAPAAWPASEPLESWALLPQKPDWAAGFRAEWTPGEAGAHARLEAFLPA
                     RAARYEERRNLPSEEGTSCLSPHLAFGEISPATLWHAAMACGGSVGTFLSELGWRDYA
                     QNIIAQFPDYGAHPARPAFAQMPWREAPEELAAWQQGRTGYPIVDAGMRQLWATGWMH
                     NRVRMIAASFLIKHLLIDWRVGERWFWDTLVDADYGQNAVNWQWSAGCGVDANQFGRI
                     MAPLVQSAKFDSAGYIRRWVPELAGLPDAEIHEPAPLARLRARYPMPIIGHAAGRARA
                     LAAYAAVRA"
     gene            complement(9924..10970)
                     /locus_tag="VFF98_14195"
     CDS             complement(9924..10970)
                     /locus_tag="VFF98_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZU64831.1"
                     /translation="MNEMTSIVKTTLKDWESDQEVRWCPGCGDYAILKAVQRTLPEIG
                     ADPAKTVFVSGIGCSSRFPYYMASYGFHTIHGRAPAVATGVKLANPELDIWLVTGDGD
                     GMSIGGNHTMHFLRRNINSQILLFNNEIYGLTKGQYSPTSRPGTPSPSTPLGSVDRPA
                     LPCAFALGAGARFVARGYDTSKELVHVLKAAHAHQGAAFVEIFQNCIVYNKDRFADFT
                     EKAVADAQQLWLKHGEPMLFAKGTKGIALNAEHLHLEVVDVVEGDWQAAGVLVHDVTN
                     RAIAHMLVELPMGAFPVALGVIYDDPRPTYEAEMLSQDARAREGKSANLAKLFAKGQT
                     WTVAAEDQGPLSGV"
     gene            complement(10982..12865)
                     /locus_tag="VFF98_14200"
     CDS             complement(10982..12865)
                     /locus_tag="VFF98_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZU64832.1"
                     /translation="MATILADTDAAARSTDNSPEAVVVRFAGDSGDGMQLTGGQFTLS
                     TALAGNDLATFPDFPAEIRAPQGTLFGVSAFQINFGSTEITTAGDQPDVLVAMNPAAL
                     KTNVGQLKAGGLIIADSGEFTKRNLEKAKYEANPLEDGSLAKWTLLSFDISALTLEAV
                     KPFGLGNKEALRCKNMWTLGLALWMFDRDRAPLVEWLKGKFAKNPVLADANIAALNAG
                     HAYGETAEIGGQVKQKHVAPVPSEPGLYRTITGAEAISLGLVAGAQLADLPMFFGGYP
                     ITPASAILHYLAKMKEFGVTTFQAEDEIAAVASAIGASYAGALGVTSSSGPGIALKGE
                     ALGLAVMTELPLVIVNSQRGGPSTGLPTKTEQSDLYQAVYGRNGDTPMPVIAARSPAD
                     AFECAIEACRIATQFMTPVMLLTDGYIGNAAEPWHVPDPASFKPFPVTFLTENNNPEG
                     GKPLPYKRDADGVRPWIKPGTPGLMHRIGGIEKAPETGDLDYSPATHQLQTDARKAKV
                     DGIAAHTPAQDIDQGTEKGRLAVVGWGSTYGPIRQAVRRARAAGKDVSHIHIRHVWPL
                     PANLGDLLRGYERILVPEMNTGQLKTVLRDQFLVDAKPLNKVSGQPFTIAEIEAAIDA
                     HLA"
     gene            13150..13659
                     /locus_tag="VFF98_14205"
     CDS             13150..13659
                     /locus_tag="VFF98_14205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZU64833.1"
                     /translation="MTTSSDYAALPYRPCVGVMLVNHQGLVFTGQRIDQRESEVGQDG
                     DFWQMPQGGVDPGEDLRAAAFRELWEETGVKESHATIIAQTREELLYDLPDHLIGKLW
                     KGKYRGQRQSWFLMRFTGEDAHIDLKAHDPAEFLAWKWVRPDQLPDLIVPFKKRVYRA
                     VLEEFRALI"
     gene            complement(13661..14203)
                     /locus_tag="VFF98_14210"
     CDS             complement(13661..14203)
                     /locus_tag="VFF98_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU64834.1"
                     /translation="MRFAPVACALSVVLALTASGGRSAPPEPLSPRAASLEAQGRSLL
                     AAGETDGAIDAFEAALALQPGAAQLVMDLAEVARRQSLQGKALHYYRQVLARDPENLF
                     AIAGEGAALAEKGATEKARRDLARLESMCGANCPATRQLASVIDRQAAQAATPAKTLA
                     DKGADAAPASAPDTAAPVKQ"
BASE COUNT         2288 a         4948 c         4716 g         2364 t
ORIGIN      
        1 ttagaagccc ctgcccctgc cgatcaagcg gcgcgcggcg gggctgtgga tatgtcgggg
       61 ggcatctgcg ggcaccggtg ggcagggcgc ggggcttggg gcagcatgcg gcgcagatgt
      121 cataattgtc atgccagtgt catcatgtaa atccgcaaga tgattgtgaa gagggcagaa
      181 atagtttccc ccgcgggcgc ttcatgcaat atcgcgccgt gtgaatgatg gccaaggggg
      241 ctttatgcag ggcacgattc tggcgttcga tttccgcagt ggcgagggtg tcatttccgg
      301 cgaagacggg ttgcgctatc gcatcaccat gcaggagtgg aaacatgccc tgccccctgc
      361 tgtcggccag acggtggatt ttcagccagc cggcgatatc gccggcgcga tctatgtgat
      421 ccagcagcca tcgcccttca gcggcgacaa gaaccggctg gtggctgcgc tgctggcgct
      481 gtttctgggg atgctgggcg ttcacaaatt ctatctgggc aagaacaccg ccggcctcat
      541 catgctggcc ggaggcacca ttggctgggt gctggtgctg ccgggccttg ccgttgcggt
      601 gatcgccttc atcgaattca tcatctatct gacgacatcc gatgccgatt tcgcggcgcg
      661 ctatgtgaac ggccccaggg cctggttctg aacggcggcg gggggcagcc ctgcccccca
      721 ggcccgcccc ctaaagcagc gcctcgatcg cgccggccag cttttcgggc gcatcggcgg
      781 gggcaaagcg gcggataacg cggccttcgc ggtcgatcag gaatttggtg aaattccact
      841 tgatgcgctg cgtgccgaac aggccgcgcg cctcgcgctt cagccattgc cacagcggcg
      901 gagcgccggg cccgttcacc tccaccttgc ccatcagcgg gaaggagagg ccgaaattca
      961 ccgcacagaa ttgcgcgatc tcttccgcgc tgcccggttc ctgcccgcca aactggttgc
     1021 agggaaaggc aatcacttca aagccctgcc cgccatagcg ccgccacagc gcctccagcc
     1081 ccttgtattg cggggtgaag ccgcatttgc tggcggtgtt caccaccagc accaccttgc
     1141 ccagccggtc ggcgagggaa atctcctcgc cactggccaa gcgggcggtg aaatcggcaa
     1201 tggtgggcgc gctggtcatg gcagggctcc tctgtagccg cgatcaggcc gcgtggggga
     1261 aatcagccag ggcgtagagt tccatcacct cggcggtgct caggcggtgg tcgctctggc
     1321 gctcgatggc ataggcatcg cgctcgccct cgggcagcag ggcgatgaag cgcaccagat
     1381 cggccagcgc gccctggcgc agcgccttca gcccatcgag caggccgccg ccatcgtcgg
     1441 cgcggtgcag gctggcggtc tctgtccagt cgatcgtgcc gggctgatag ggctcgccac
     1501 ggaaagtgct gggcggttgg ctcatttctg caaaatcctc tctggtcgct ctgcccgccc
     1561 ggtgccgatg gcgaggcggg cacttgttgc atgtgatcct tcgcagggcc gctgacaagc
     1621 cctgccaaag gcgcaaatca cccctgctgg cgcgcgcctt cttccagccg cccctcgtgc
     1681 aggcgcacca cccggtccat cgcggcggcc aggcgctcgt tgtgggtggc aatgagggcg
     1741 gcgctgccca gcccgcgcac cagctccaca aactggccca gcacgatatc ggccgtcgcc
     1801 tcgtcgagat tgccggtggg ctcgtcggcc agcaccagcc gggggcggtt ggccagggcc
     1861 cgggccaccg ccacgcgctg ctgctcgccg ccggaaagct ggctgggccg gtggccaagc
     1921 cgctgcccca gccccagcgc ggccagcaat tcctgcccgc gcgcctcggc cgcggggcgg
     1981 gcggtgccgg cgatcatctg cggcagcacg acattttcca gcgcggtgaa atcgggcagc
     2041 agatggtgga actgatagac aaagccgatc gcctcgcgcc gcagccgcgt gcgcccctcg
     2101 ccatcgagcg cggccgcttc tgccccgcca atgcggatgg agccgccaaa gcccccctcc
     2161 agcaggccca ccgcctgcag cagcgtgctc ttgcccgagc ccgatggccc cagcagcgca
     2221 acgatctcgc ccgggcggat cgccagatca acgccgcgca gcacatccag cgccacgccc
     2281 ccttgcgtga agcgccgggt gaccccggcc agctcgacga tcgcctcact cataacgcag
     2341 cacctgcacc ggatcggtcg acgccgcctt cagcgccgga tagagcgtgg ccaggaagct
     2401 gaacagcagc gccatgccgg caatcaccgc aatctccacc ggatccggcc ggctgggcag
     2461 ttcggtgagg aagcggatct tgggatccca caggttctgc ccggtgatga actgcaccgc
     2521 attcaccaca ttctgccgga aatagaggaa gacaaagccc agcagcaggc ctgccaccgt
     2581 gcccgccgcg ccaatcacga aaccggtggc aacaaagatc ttcagcaccg accggctgct
     2641 tgcccccatc gtgcgcagga tggcgatatc gcgcgttttg gcgcgcacca gcatgatgag
     2701 gctggagagg atgttgaaca ccgccaccag cacgatgatc gagagcacga cgaacatgct
     2761 cacccgctcc accgccagcg cctcgaacaa ttgcgcgttg agctgctgcc agtcagtcac
     2821 caccgcccgg cccgacagcg tctgggccag cggcgccagg atctcgccgg ccttctcggc
     2881 attgctggtg gtcacctcga tcatgccgat cgtgtcgccg gaaaggagca gcgtctgcgc
     2941 atcctcgatc ggcatcacca cataggtgtt gtcgtagtcg tagacgccga tctcgaagat
     3001 cgccgccaca tgataactca cctgccgggg cacggtgcca aagggcgtgg cgcggcccac
     3061 cgggttgatc acggtgatgt catcgcccac ccgggcgccc atcgcctcgg ccagccgcgc
     3121 gccgatcgcc acattgtcgc tgcccgggct gagatcggcc agattgccgg cggccagctt
     3181 gggcttcagc cgggcgatat cgggcgcggt gttgccgcgc accagaatgg cctccacccg
     3241 cccgttgaaa ctggccagca gcggctgctc gaccagcggg ctggcatgga tgacgccggg
     3301 cgtcgcctcg gtctgcttca gcaccgcgcg ccagtcgtcc agccggccgc cataggcctg
     3361 gatgatggca tggccgttca gcccgacgat cttgtcgatc agctcacccc tgaagccgtt
     3421 catcacgctc atcacgatga ccagcgctgc cacccccagc atcaccgcaa ccaggctgat
     3481 gcccgccacc agcgcgatga aacgctcgcc cttgccgggc agcatgtagc gccgggccat
     3541 ggtccattca aaaggggaaa gcagcaaggt ggggcccgtt acatctgata aggcgccgcc
     3601 agcgccggac agctgcccgt ttaggcgggc cttggcgctg gcgcaatcgc ttttgcccgc
     3661 ccttttcgcg cgccccttgc ccgccgccgg caccgcccgc gccatatcag ggtttatacg
     3721 ttcttaacca tgcatgccta agcaggggca catgatttgc tccacccgta aacgcgccct
     3781 gttcctgccc gttatcggct atgccgcggc ggcctgcctg gccttgcgct ttgcccgttt
     3841 cgatggcggc gtggcctatg tgtggggggc caatgcggtg ctggcggcgg tgctttcaac
     3901 cctgccgcgc gggcgctggt gggaagtgct gctgccctgc ttttttgcgg gctggctaac
     3961 caatggcctg tgggggctgg gctggcagct ggcgccgggg ttggcctttg gcaacatggc
     4021 cgatgtggtg gtggccgttg ccctgctgcg ccggttgacc cgcaccgccg atgcccgcat
     4081 ccccaaggcc gagccgaccc cgccactgct gcctttcgtg gcggcgatgg gccttgccgg
     4141 ccctgccacc agcacgctgc tggccaccct caccgtgatg ctcagcggcc ggccgccatc
     4201 ggcgccgctg gcgctgacgt ggttcatcgg ccatggcctg ggcatgctgg ccttctatcc
     4261 ggggctgctg gtcatcgtgc gcggcacccg gcgcgcgggc acgccgctgg cggaaaaggc
     4321 cccgcgccat cgggcctggc cgcgccaggc cggcgtgctg ctgctgttca ccgccgcctg
     4381 cctctacacc tttgcccagc agggccggcc gctgctgttt gtgcccacgc tggtgctggc
     4441 cgcggccatc gcctgggccg aaaccgccac gctggcgctg gcttatggca ttctggcgct
     4501 ggtgggcggg gcgttcaccg ccatgggcca tggccccatc gcgctgatgg cggtttccat
     4561 gccgcagcgc atgcagatgc tgcagatcta tctggcgctc agcgtggtgt gcacgctgcc
     4621 gatggcctcc atcgtgcacc ggcgggtgca gctgctggcc cgtatgcgcg aaagcgaggc
     4681 gcgctatcgc ctgctggcgg acaattccac cgacatcatc ctcagcaccg atgcggccgg
     4741 gcgcatccgc tttgtcagcc cctcggtgct gctgctggcc catcatcagc cggcagagct
     4801 gatcggccgg ctggcgcgcg atttcgtcgt gcccgagcac cgcgcccagg cgatcgctgc
     4861 ccatctggcc gccatgcgcg cgccgggcag caccatcacc gccgaattcc ttggcctggt
     4921 gcgctatggc gagccgcgct ggttcgaaag ccggatgcgc gcggtggccg gcgatgatgg
     4981 ccgggtggaa tgcgtggtca atgtcgtgcg cgacatgtcg cggcgcaagg aatatgaaac
     5041 cgcgctggcg ctggccgccc tcaccgatcc gctgaccggc ctgcccaacc gccgcctgtt
     5101 ccgcaacgcg ctggaggatt gcatcgccaa tgccggctgc ggctgcgtgg cgctgatcga
     5161 tctcgaccat ttcaagctgg tgaacgaccg ccacggccat gccgccggcg atgcggtgct
     5221 gaaaagcgtg gccgcggccg cccgcgcggc gctgcgcggc agcgacacgt tcgcccggat
     5281 gggcggcgag gaatttgccc tgctgctgcc cggcgccagc ctggaagtgg cgcagggcgt
     5341 gtgccggcgc ctggcctcca ccctggccgc caccactacc gagcacgagg gccaggcgat
     5401 ccgcatcacc tgctctgtgg gcctggcgcg gctggggcgc gatgccgata ccgccatggc
     5461 cgcggccgac cgcgcccttt atgacgccaa ggccgccggc cgcgattgcc tgaaaatcgc
     5521 cgcctgagcg cagccccggc cgctctggcc ggcgcggctc ccacgcctcc tgccccacac
     5581 ctcttgcgtt tggcggcaaa cagggccaaa ggcgcccgtg cctggggcgc atcgcgaggg
     5641 tgctggcccc ttgtgccctt tgtatcgccc gacccccaat cgcccgaccc caacgagaaa
     5701 gctgctcctg gcatgaacgc aagcccatcg ccccgtgata ccaccccctg ggacatcgcg
     5761 ccggatggcg atggcggcga ccatctgcat gaggaatgcg gcattttcgg cgtgatcggc
     5821 gcgcgccagg cggcctcgat ggtggcgctg ggcctgcatg cgctgcagca ccgcgggcag
     5881 gaagcggtgg gcatcaccag cttcgatggc acagaattct tctcctatcg cggcatcggc
     5941 catgtggccc atgtgttcaa caaccaggat atcttcgccg atctgcccgg cggcatggcc
     6001 tcgggccatg tgcgctattc caccaccggt ggctcagggc tgcgcaatat ccagccgctg
     6061 tttgccgatc tggcctcggg cgggttttcc atcgcccata acggcaatat ttccaatgcc
     6121 atgaccctga agcgcgatct cgtgcagaag ggctcgatct tccagtccac ctccgacacc
     6181 gaggtgatca tccacctcgt ggcgaccagc cgctacccca ccctgctcga ccgtttcgtc
     6241 gatgcgctgc ggctgatcga gggcgcctat tcgctgatcg tgatgacgcc cgaaggcatg
     6301 atcgcctgcc gcgacccgct gggcatccgc ccgctggtga tgggccgcat tggcgacagc
     6361 gtggtgtttg ccagcgaaac cgtggcgctg gacgtggtgg gcgcggaatt cctgcgcgag
     6421 gtggaaccgg gcgagatgat cgaggtggat cacaagggcg tgatctccag ccaccgcccc
     6481 ttcggcaccc agccggcgcg cccctgcatc ttcgagcatg tctatttcag ccgccccgat
     6541 tcggtgatgg gcgggcgcag cgtgtatgaa gcgcgcaagg cgatcggcaa ggaactggcc
     6601 aaggaagccc ccgccgatgc cgatctcgtc atcccggtgc ccgattcggg cgtgccggcg
     6661 gcgatcggct atgcccagga aagcggcatc cccttcgagc tggggatcat ccgcagccac
     6721 tatgtgggcc gcaccttcat ccagccttcc gatggcgcgc gccatgccga tgtgaagcgc
     6781 aagcacaatg ccaaccgcgc gctggtgcag ggcaagcgca tcgtgctgat cgacgattcg
     6841 atcgtgcgcg gcaccacctc gctgaagatc gtgcagatga tgcgcgatgc cggcgccagc
     6901 gaagtgcaca tgcgcatcgc cagcccgccc accgagcaca gctgcttcta tggcgtcgac
     6961 acgcccgagc gttccaagct gctggccgcg cggatggatc ttgatgcgat ggccgagttc
     7021 atccacgccg acagcctggc cttcatctcg atcgacgggc tctatcgtgc cgtcggcgag
     7081 gccgagcgca agccggcctg cccgcagttc tgcgatgcct gcttcaccgg cgattatccc
     7141 acccgcctca ccgatcttgt cgccgccggc aaggccgatg gcgtgaacca gccggactga
     7201 gccgccgtga gcgaagagac ccagcccttt gccggccagc tggcactggt gaccggcgcc
     7261 agccgtggca ttggcgcgga aaccgccaag gcactggccg ccgccggcgc ccatgtggtg
     7321 ctgaccgcgc gcgacacccg cgccctggaa gcggtggagg aagagatcca ccgcgccggc
     7381 ggccatgcca ccatcgcccc gttcgacctg gccgagcccg atgccatcgc ccgcctggcc
     7441 gcggccatca ccgaacgctg gcccgcgctc gatatcctcg tcatcaatgc cggcgtgctg
     7501 cccacgctga cgccggttac ggatatcgat cccaaggcgt tcaaccgggc gatcgcggtg
     7561 aatgtgctgg ccacccaggc gctgcttgcc tgcttcaccc cgctgctgcg caagagcgcc
     7621 gatgcacggg tgattggcct taccacttcg gttgccggca ccccccgcgc ctattggagc
     7681 gcctatggcg ccaccaaggc cgcgcaggaa gtgctgctga ccagctatgc ccaggaaatg
     7741 cgcaacattt ccaatatccg cgtggccatc gtcaaccccg gcgccacccg cacggcgatg
     7801 cgcgcccgcg cctatccggg cgaggatccg gccagcgtga agccgcccga agtggtggcc
     7861 cgccgcctgg ttggcctgtt gacggaaccc ttcgtctccc cctcgcgcga ggatattccc
     7921 aagggctggt agggccgcgt taacgcccct tgcggcatga tgcagggcaa tggcggtggc
     7981 gcgggccacc ccaagccgga gcctttttct gcatgtcgca aagccagccc tctccccttg
     8041 ccgcgcgcct cagcccgcag cgccgcgccc atctgcgcgc cgtcgaggaa cgcgtctcgc
     8101 agctgacggt gatccagccg cggcccgcag aagaccagca cgaacagcgc gccgccccgc
     8161 gcatccgcgt ggccgtgccg gtgatcatca cgctgcacac cgggcaaagc ttccattcgg
     8221 tcgtgcatga tctgtcgatc tcgggcttca gcgcctcggc gatggtgcgc atggagccgg
     8281 aaagccgctg catcatgaag ctgccgggcc tggccgggcg gcaggcgcgg gtggtgtggt
     8341 ggaatgccag catcgttggc tgcgcctttg ccgaaccgct gaccaccgcc acgctggacg
     8401 agctggtgga gcgctatcgc cacgccgcca ccctgcaccc cagcgcctga cctcctaggc
     8461 acgcaccgcc gcataggcgg ccagcgcccg cgcccgcccc gctgcatgac cgatgattgg
     8521 catcggatag cgcgcgcgca ggcgcgccag cggcgccggc tcgtggatct cggcatcggg
     8581 caggccggcc agttcgggca cccagcggcg gatatagccg gcgctatcga acttggcact
     8641 ttgcaccagc ggcgccatga tccggccgaa ctggttggca tccaccccgc agccggccga
     8701 ccattgccag ttcaccgcat tctgcccgta atcggcatcc accagcgtat cccagaacca
     8761 gcgctcgccc acccgccagt cgatcagcag atgcttgatg aggaagctgg cggcgatcat
     8821 ccgcacccgg ttgtgcatcc agccggtggc ccacagctgg cgcatcccgg catcgacgat
     8881 gggatagccg gtgcgcccct gctgccaggc ggccagctcc tcgggcgctt cgcgccaggg
     8941 catctgcgca aaggccgggc gcgccggatg ggcgccataa tccggaaact gggcgatgat
     9001 gttctgcgca taatcgcgcc agcccagctc cgacaggaac gtgccgacac tgccgccgca
     9061 tgccatcgcc gcatgccaca aggtggcggg gctgatctcg ccaaaggcca gatggggcga
     9121 gaggcagctg gtgccctcct ctgacggcag attgcgccgt tcctcatagc gggcggcacg
     9181 ggccggcaag aaggcttcca gccgggcgtg ggcgccggcc tcgccgggtg tccattccgc
     9241 acgaaaaccg gcggcccaat cgggcttttg cggcagcagg gcccagcttt ccagcggttc
     9301 gctggccggc caggcggcgg gcgcggggat agcgccgggc gctgcctgcg gcaccggcgg
     9361 cggcatatgc agctgcagcg cgcgccagaa gggcgtgtag atgcgaaagg gcgtgccctg
     9421 cccggtggta acgctgcccg gcggcaggag gaaattgcca tggtgcagct gcagatccag
     9481 ccctgcccgg cgcagcccct tttcggcatt cagccaccag ggctcgtaat ggtgcagggc
     9541 gtggagggtg gtggcgccca cttcggcggc cagcgccggc agcacatcgg ccaccgcgcc
     9601 ccggcgcagc accagccggc tgccccgcgc ggcaagatca ccggcaaggc tagcaaggct
     9661 gccatgcagc caccagcgcg aggcaccacc cagcgcgcga tggcgcgggg tgtcatcatc
     9721 cagcacatag acggggatga ccggcgcgcc gctggcacag gcggccagca cggcagcctg
     9781 atcggccagg cgcagatcgc ggcgaaacca caggagaacg ggagcttggg gcatggcctg
     9841 tgctatacag gctggcggtc aggcgcgccc tgccctttgg ggcaatggtg gccgggaaga
     9901 tcctccccgg ccccatctgc cgatcaaacg cccgagagcg ggccctgatc ttctgctgcc
     9961 acggtccagg tctggccctt ggcgaacagc ttggcgagat tggcgctctt gccttcgcgc
    10021 gcgcgggcgt cctggctcag catctcggcc tcgtaggtgg ggcgcggatc gtcgtagatc
    10081 acgcccagcg ccaccgggaa ggcgcccatc ggcagttcca ccagcatgtg ggcgatggcg
    10141 cggttggtca catcatgcac cagcacgccg gcggcctgcc agtcgccctc caccacatcg
    10201 accacctcga ggtggagatg ctcggcattg agggcgatgc ccttggtgcc cttggcaaac
    10261 agcatcggct caccatgctt cagccacagc tgctgggcat cggccaccgc cttctcggtg
    10321 aagtcggcaa agcggtcctt gttgtagacg atgcagttct ggaagatctc gacgaaagcc
    10381 gcgccctggt gggcatgggc ggccttcagc acatgcacca gctccttcga cgtatcgtag
    10441 ccacgcgcca cgaagcgggc gccggccccc agcgcgaagg cgcagggcag cgccggccgg
    10501 tccaccgagc ccagcggggt ggaggggctg ggcgtgccgg gccggctggt ggggctgtac
    10561 tgccccttgg tcaggccgta gatctcgttg ttgaacagca ggatctgcga attgatgttg
    10621 cggcgcagga aatgcatcgt gtggttgccg ccgatcgaca tgccgtcgcc gtcgccggtc
    10681 accagccaga tgtcgagctc ggggttggcc agcttgacgc cggtggccac cgccggcgcg
    10741 cggccgtgga tcgtgtggaa gccataggag gccatgtagt aggggaagcg gctggagcag
    10801 ccgatgccgc tgacgaacac cgtcttggcc ggatccgcgc cgatttccgg cagggtgcgc
    10861 tgcaccgcct tcaggatcgc atagtcgccg cagcccgggc accagcgcac ttcctgatcg
    10921 gattcccagt ccttgagggt ggtcttaacg atggaggtca tctcgttcat ggcaaaatcc
    10981 ctcaggccag gtgcgcgtcg atcgccgctt cgatctcggc gatggtgaag ggctggcccg
    11041 acaccttgtt gagcggcttg gcatcaacca ggaactggtc gcgcagcacg gttttcagct
    11101 ggccggtgtt catctcgggc acgagaatgc gctcatagcc cctgagcaga tcgcccagat
    11161 tggccggcag cggccacaca tggcggatgt ggatatggct gacatccttg cccgccgcgc
    11221 gcgcccggcg cactgcctgg cggatcggcc cataggtgct gccccagccg acaacggcca
    11281 ggcggccctt ctcggtgccc tggtcgatat cctgcgcggg ggtgtgggcg gcaatgccat
    11341 ccaccttggc cttgcgggcg tccgtctgca gctggtgggt ggcgggcgaa tagtcgaggt
    11401 cgccggtctc gggcgccttc tcgatgccgc caatgcggtg catcagcccc ggcgtgcccg
    11461 gcttgatcca cgggcgcacg ccatcggcat cgcgcttgta gggcaagggc ttgccgcctt
    11521 cggggttgtt gttctcggtc aggaaggtca ccgggaaggg cttgaagctg gccggatcgg
    11581 gcacatgcca gggctctgcc gcattgccga tatagccatc ggtcagcagc atcaccgggg
    11641 tcatgaactg ggtggcgatg cggcaggcct cgatcgcgca ttcaaacgca tcggccgggc
    11701 tgcgcgcggc gatcaccggc atcggcgtat cgccattgcg gccatagacg gcctggtaaa
    11761 gatccgactg ctcggtcttg gtgggcaggc ccgtggaggg gccgccgcgc tgcgaattga
    11821 cgatgaccag cggcagctcg gtcatcaccg ccaggcccag cgcctcgccc ttcagcgcaa
    11881 tgcccgggcc cgaggaggaa gtgaccccca gcgcgccggc ataggacgcg ccgatcgccg
    11941 aggcgacagc ggcgatctcg tcttccgcct ggaaggtggt gacgccgaac tccttcatct
    12001 tggcgagata gtgcaaaatc gccgaggcag gggtgatcgg atagccgccg aagaacatcg
    12061 gcagatcggc cagctgggcg cccgccacca ggcccaggct gatcgcctcg gcgccggtga
    12121 tggtgcgata gaggccgggc tcggaaggca ccggcgccac atgcttctgc ttcacctgcc
    12181 cgccgatctc ggccgtctcg ccataggcgt ggccggcgtt cagcgcggcg atgttggcat
    12241 cggccagcac cgggttcttg gcaaacttgc ccttcagcca ttccaccagc ggcgcgcggt
    12301 cgcggtcgaa catccacagc gccaggccca gcgtccacat gttcttgcag cgcagcgctt
    12361 ccttgttgcc aaggccaaag ggcttcaccg cttccagcgt cagcgcggaa atgtcgaaag
    12421 acagcagcgt ccacttcgcc aggctgccat cctccagcgg gttggcctca tacttggcct
    12481 tctcgaggtt gcgcttggta aactcgcccg aatcggcgat gatgaggccg cccgccttca
    12541 gctggccaac gttggtcttc agcgcggccg ggttcatcgc caccagcacg tcgggctgat
    12601 cgcccgcggt ggtgatctcg gtgctgccga agttgatctg gaaggccgaa accccgaaca
    12661 gcgtgccctg cggcgcgcgg atttcggcgg gaaaatcggg gaaggtggca agatcgttgc
    12721 ccgccagcgc cgtcgaaagc gtgaactgcc cgccggtcag ctgcatgcca tcgccggaat
    12781 cgccggcaaa gcgcaccacc accgcttcgg gggaattgtc ggtcgaacgc gcagcagcgt
    12841 ccgtatcggc caaaatcgtt gccatcttca accttttcat ctgcgcacgc cttatccggc
    12901 gcccgcgcgc tggcgccccc atgcgccttt cgccccacca gcacaatcgc gaaaaagctg
    12961 tgcggggccc aaggccagct tttcgcagaa cgcctgttgc gcctgccagt caagcccctt
    13021 tcccgccgtt cgcgccggcc gcgcgtggcc agcatgacac aactgtcatg aaagcgggtg
    13081 ataaccgcca cgcgcagggc cgggcgatgc ttgaaattcc cgcgcgttca cagcaagtac
    13141 aggtctatca tgacgacatc ttccgattat gccgccctgc cctaccgccc ctgcgtgggg
    13201 gtgatgctgg tcaaccatca ggggctggtg ttcaccggcc agcggatcga ccagcgcgag
    13261 agcgaagtgg gccaggatgg cgatttctgg cagatgccgc agggcggggt ggatccgggc
    13321 gaggatctgc gcgcggcggc ctttcgcgaa ttgtgggaag agaccggcgt caaggaaagc
    13381 cacgccacga tcatcgccca aacccgcgag gaactgctct acgacctgcc cgatcacctg
    13441 atcggcaagc tgtggaaggg caaatatcgc ggccagcggc aaagctggtt cctcatgcgc
    13501 ttcaccggcg aggatgccca tatcgacctc aaggcgcatg atccggccga atttctggcc
    13561 tggaaatggg tgcgcccgga ccaattgccg gatctgatcg tgcccttcaa gaagcgcgtc
    13621 taccgcgctg tgctggaaga attccgcgcg ctgatctgaa ctactgcttc actggcgcgg
    13681 cggtgtcggg cgcgctggca ggggcggcat cggcgccctt gtcggccagc gttttggcag
    13741 gggtggcggc ctgtgccgcc tgccggtcga tcacgctggc cagctggcgg gtggcggggc
    13801 aattggcgcc gcacatgctc tccagccggg caaggtcgcg ccgggccttt tcggtggcgc
    13861 ccttctcggc cagcgcggcg ccttcgccgg caatggcaaa caggttttcc ggatcgcgcg
    13921 ccagcacctg gcgatagtag tgcagcgcct tgccctgcag gctttgccgg cgggccactt
    13981 cggccagatc catcaccagc tgcgccgcgc ccggctgcag cgccagcgcc gcctcgaagg
    14041 catcgatcgc cccatccgtc tcgccagcgg ccaagagcga gcggccttgc gcttccagcg
    14101 aggcggcgcg cgggctcagc ggctccggcg gcgccgagcg gccaccgctg gcggtgagcg
    14161 ccagaacgac cgacaaggca caggcaaccg gggcaaaacg catcaacaat tccttggccg
    14221 aagcgcgcgg agaaccgcgg cgatgaagac gcagatcgga catttccgcc aaggctagca
    14281 caggctttgc ccccgtgcga tgaaaaagcc gtcggt
//
LOCUS       DASRYE010000276         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_211359,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000276 DASRYE010000000
VERSION     DASRYE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211359"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFF98_14215"
     CDS             complement(<1..693)
                     /locus_tag="VFF98_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64835.1"
                     /translation="MPWLAAALLPAPAAWAGSAAARPPAAAAAAGFLQKTFETGPFSA
                     DNVDLANSGKKGFNWYFWNSFGCTATAADAHLAIGDDGALTLGGACGNGSLVSGAARP
                     SDPQRFVGQAFGGGGYFEAEIRIDTTRPQQGHGWPSWWLLSAEHMWYLPQNYWPGQTN
                     GYEHFLEIDAFEAMRPAGRADAYMATVHDWYGTFHKTCPPKAAFCQIITPYHDNIVAP
                     ARPIDWSRWHRIA"
     gene            complement(775..2043)
                     /locus_tag="VFF98_14220"
     CDS             complement(775..2043)
                     /locus_tag="VFF98_14220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZU64836.1"
                     /translation="MLQTVLITLSLIALLFQGLPGKGTACQLAAIGLNVICSLTYLPH
                     AFRGQRFSRFEMVAVALCIVSFLTLTIQQIVNEKDFIDFSLSVTALMTVTVLSVSINA
                     RIIPIESFMNCVAMAAIASTLAMYAVYPGDILASLTSSGATRWALRFSGFENHPNLEG
                     LIFGSYILYIALFALREGRIRWQYLAFVPLALGIILAAGARGGLLALLISSLIVLITL
                     WPRIDNATRRRIFTAGGLALVIALAFSGKLYDYLTVVLELDSKERGFGSGASGRTDIW
                     AEGISMIGASARSAVLGGGLRSSDFIRSDFYTESSYISILLDSGIIFGGTFILMVLGS
                     AVIAGKRARARPSALNIAVFMLLNYGIFESFFNRYLFAMGNPFSVSMMYMLAYVNNGA
                     LDKTPAVAAGRRLYYIVSTHSHWPPRPSES"
     gene            complement(2058..>2383)
                     /locus_tag="VFF98_14225"
     CDS             complement(2058..>2383)
                     /locus_tag="VFF98_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64837.1"
                     /translation="AIADPMYSILSTRNAHTRVTGIYLASSLAALALAWLLTRWHASM
                     VEVAACVVLAELSNLLAGYYFAARMFRMPLNRFVPSLLRLGAQRLKSMPGSVKRKLQA
                     NGQAS"
BASE COUNT          456 a          685 c          887 g          355 t
ORIGIN      
        1 ggcgatgcgg tgccacctgc tccagtctat cggccgggcg ggggcgacga tgttgtcgtg
       61 atagggggtg atgatctggc agaaggcggc cttggggggg caggtcttgt ggaaggtgcc
      121 gtaccagtcg tggacggtgg ccatataggc atcggcgcgg ccggccgggc gcatggcctc
      181 gaaggcgtcg atctcgagga aatgttcgta gccgttggtc tgtccgggcc aatagttctg
      241 cggcagatac cacatgtgct cggccgacag cagccaccag ctgggccagc cgtggccctg
      301 ctgggggcgg gtggtgtcga tgcggatctc ggcctcgaaa tagccgccgc cgccaaaggc
      361 ctggcccacg aagcgttgcg gatcggacgg gcgggctgcg cccgagacaa gcgagccatt
      421 gccgcaggcg ccgcccagcg tgagcgcgcc atcatcgccg atggccagat gcgcgtcggc
      481 cgcggtggcg gtgcagccga agctgttcca gaaataccag ttgaagccct tcttgccgga
      541 attggcgaga tcgacgttgt cggccgagaa ggggccggtc tcgaaggttt tctgcaggaa
      601 gccggcggca gcggcggcgg ccggcgggcg ggccgcggcg ctgcccgccc aggccgcagg
      661 ggccggcagc agggccgcgg caagccaggg caacaggccg gcgcaccatg ccggttgccg
      721 gagaaggggc tgggcggggc aggcgcggcg ctgaggcatg ggggcggtcc ttagctagct
      781 ttcggaaggg cggggtggcc agtgcgaatg ggtgctgacg atataataca ggcgccggcc
      841 ggccgcgacg gcgggtgtct tgtcgagcgc gccattgttc acataggcca gcatatacat
      901 catgctgacc gagaaggggt tgcccatggc gaacagatag cggttgaaga aggattcgaa
      961 gatgccgtag ttcagcagca tgaagacggc gatgttgagc gcgctgggcc gggcccgcgc
     1021 ccgcttgccg gcgattaccg ccgagcccag caccatgaga atgaaggtgc cgccgaagat
     1081 gatgccggaa tcgagcagga tgctgatgta ggagctttcg gtgtagaagt cggagcggat
     1141 gaagtctgac gagcgcaggc ccccgccgag caccgcgctg cgcgccgagg cgccgatcat
     1201 cgagatgccc tcggcccaga tgtcggtgcg gcccgaggcg cccgagccga agccgcgctc
     1261 cttggaatca agctcgagca cgacggtcag gtagtcgtag agcttgccgg agaaggccag
     1321 ggcgatgaca agggcaaggc cgccggccgt gaagatgcgc cggcgcgtgg cgttgtcgat
     1381 gcgcggccac agcgtgatca gcacgatcag cgaggagatc agcagcgcca gcaggccgcc
     1441 gcgcgcgccg gcggccagga tgatgcccag cgccagcggc acgaaagcca gatattgcca
     1501 gcggatgcgt ccctcgcgca gggcgaagag ggcgatgtag aggatgtagg agccgaaaat
     1561 cagcccttcg aggttggggt ggttctcgaa gccggaaaag cgcagggccc agcgcgtggc
     1621 gcccgaactg gtgagcgagg cgaggatatc gccgggatag accgcataca tggccagggt
     1681 gctggcgatg gcggccatgg ccacgcagtt catgaaggat tcgatcggga tgatgcgcgc
     1741 gttgatcgat accgagagca ccgtcaccgt catcagcgcg gtgaccgaca gggagaagtc
     1801 gatgaagtcc ttctcgttga cgatctgctg gatggtgagg gtgaggaagc tgacgatgca
     1861 cagcgccact gccaccatct cgaagcgcga gaagcgctgg ccgcgaaagg catgcggcag
     1921 ataggtgagg ctgcagatca cgttgaggcc gatcgcggcc agctggcagg cggtgccctt
     1981 gccgggcagc ccctgaaaca gcagcgcgat cagcgacagc gtgatcagca cggtttgaag
     2041 caaggtcgct gatttcttca tgacgcctga ccgttggcct gcagcttcct tttcacgctg
     2101 cccggcatgg atttcagcct ttgtgcgcca aggcgcagca gagacgggac aaagcgattg
     2161 agcggcatgc ggaacatgcg cgccgcgaaa taatagccgg ccagcaggtt ggacaattcc
     2221 gccagcacca cgcaggcggc cacctcgacc atgctggcat gccagcgcgt gagcagccag
     2281 gccagcgcaa gggcggccag cgaactggcc agatagatgc ccgtgacccg cgtgtgggcg
     2341 ttgcgcgtgg acaggatcga atacatcgga tcggcaatgg ccc
//
LOCUS       DASRYE010000277        14382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_87970,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000277 DASRYE010000000
VERSION     DASRYE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87970"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFF98_14230"
     CDS             <1..982
                     /locus_tag="VFF98_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZU64838.1"
                     /translation="SAPLKARIARHYAETAGVEVAPERIILTCGASPALVLALASAFA
                     PGDQVALARPGYVAYRNTLRALHMVPVEIGCGPETRYQLSAAQIAALDPAPAGLILAS
                     PANPTGTILPPAELAAIAAVCAARGIRIVSDEIYHGLSFGLPTQTVLAHAPDALVVNS
                     FSKYFSMPGWRLGWLVAPADLAQAAYARMSNLFLTPPVLSQHAGLAAFDGQAELEGHI
                     ATYAANRALMLAALPRLQLSTIAPPDGAFYIWADIAHLLAPGENSMDFAIALLRETGV
                     ATAPGLDFDPVEGGHFLRFSFAVATPLVAEAIERMVPFFAARARPEGAPL"
     gene            1041..2051
                     /locus_tag="VFF98_14235"
     CDS             1041..2051
                     /locus_tag="VFF98_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU64839.1"
                     /translation="MAGHGWVWAAGLAGLAALGGALAWAWRPLPVEVARPTRGPAADL
                     VYATGYVEAAEPVLVVARLTAPVLKVLVAEGQAVRKGQPLAILDAGEQRALAAQTAAQ
                     ERAATLAEGRSLALFGDGWVTKAARDAAVADADAARAAHAAAAARIDQLVVRAGIDGI
                     VTKRDIYPGELASPSRTLFQLGDPARQRITATLDERDIARIAPGQPALMSSDAWPGRV
                     LHAHVAEITPGGDPTTRAFRVRLQADEHTVLPMGLTLEVNIIARQHPHALLLPASAVS
                     GGRVWVVAQGRARPRAITTGIAGADRVEITGGLGDGDEVILHPPASLADGTRVAPQGP
                     VH"
     gene            2055..3305
                     /locus_tag="VFF98_14240"
     CDS             2055..3305
                     /locus_tag="VFF98_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64840.1"
                     /translation="MLARARLLLHIALRHLLVRQRQTLVATSGVAVGVGFFLAVSAMM
                     IGSQADFIRTLIDAAPHITLSDELRSPRPQPGAIAFAGGAVALHGYKLRNEVRGLKDW
                     QAILADINATPGAIGSPSLAGAVTLRLGGREEPLEVVGVEPAIETRVSTIADKLRAGR
                     LDDLERVQGGLIIGEEMAGRLGLNMGDIIAATSAGGTTRSLRIVGLVKKGNTQIGDSA
                     GYMLLREAQSLLGRPFIINRIGVHLADPYAAQAIAARLEAQYGYKAQSWQERSADFLA
                     LLVTRNIIMYTVVSAILLVASFGIYTAVSNSVADKKRDIAILRSMGFSSGDLQAIFVI
                     EGLMLALVGVALGWALGTGLMDVLGALTFSIGGDPQKIPLDRSGRQYAVAAAASLLAG
                     TLAAWLPARKTARLDPVDILRGAV"
     gene            3302..3988
                     /locus_tag="VFF98_14245"
     CDS             3302..3988
                     /locus_tag="VFF98_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZU64841.1"
                     /translation="MSLLLEARGLSRQLPGDPPVRLVEEVNLAIRPGAFTAIVGPSGC
                     GKSSLLYLLGLIDRPSSGQLLLEGVDVAPLSGDERARLRLERFGFVFQFHFLLPEFSA
                     LENVLLPIRRLGRLAGPAATARAMALLEGVGLGEKAGKTPEKLSGGERQRVAIARALA
                     NDPALILGDEPTGNLDSANSARVVEMLRGLAHGEARAVVCVTHDPRIAEIADVRVEML
                     DGRVVAIEEN"
     gene            complement(4068..5123)
                     /locus_tag="VFF98_14250"
     CDS             complement(4068..5123)
                     /locus_tag="VFF98_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU64842.1"
                     /translation="MKNRRIVLASRPSGVPVPANFRLEEVDVPELAEGQVLIENLVFA
                     IDPAVRGMLDDVRSYMPPVPIGGLIPTMVLGRVVASRNPDFHEGDYGRAFIGWEEFSV
                     LTPGAVGFENVRIAPDMPLTAYMGALGWSGITAYVGLKRYGEMRAGDEVLISAAAGAV
                     GSVAGQVARLSGCRAVGTVGGADKARIAREVLGFDDVIDYRAAPDLAEAIRAAFPNGI
                     DLYFDNVGGETLDAVLPQMKAFGRIPVCGMISNYNHQNEPYLLRNIWQVLVNRITMRG
                     FLAYEAADMLHEAEDALADWVRKGQLIATENVTTGLEHTPEAFIRLMSGTTTGKTLVR
                     LREDISTLESLKVGQPA"
     gene            complement(5158..5460)
                     /locus_tag="VFF98_14255"
     CDS             complement(5158..5460)
                     /locus_tag="VFF98_14255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZU64843.1"
                     /translation="MIFVAGTLSIDPAQLDAFAADVAEMRPKVLEEEGCRHYSLLVED
                     AAKGEINVLEIWDSEEHLKVHLTMPWITTFFNRYSPHITGMTAQVYDAANPRAVPL"
     gene            complement(5618..6103)
                     /locus_tag="VFF98_14260"
     CDS             complement(5618..6103)
                     /locus_tag="VFF98_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU64844.1"
                     /translation="MSKSNEPATAALADIYHHGLLVADLAATMAALGPALGVEWAPVR
                     SFSPMPMWLADGTEAVAHLKVAYSRQGPIHLELIEARPGDAYGALRAENQAHLGVWVE
                     DLAGEAEALLAAGWRMVLSGAPAASGYGLIAYLAPPDGGPVVELVSAALRGAMAEWWA
                     S"
     gene            6293..7525
                     /locus_tag="VFF98_14265"
     CDS             6293..7525
                     /locus_tag="VFF98_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64845.1"
                     /translation="MPSPAAAELCPPPADDLARMAWERARKAPPEGFPALPDIPGQRY
                     VNEAFLSLEEQRMWQRAWLYAGHTDQLPTPGSWFLTRNTGSPVIVARTLSGEIRAYYN
                     TCRHRGAPLVTEAAGEARGFMCSYHGWSYTLEGRLTAIRDARDFVDFDLACHSLVPVR
                     CELLGSLIFLNQDAQAQPLLDHIGPMAKELEQLQLDKLRLVDSRSYDVDCNVKVLLDA
                     FLEVYHLKSIHVNTVDRFLDHRAMIVTLWPNGHSRMATPNRREGWTDPGTVGMPEIPT
                     VSEFLAKNNVSYALYPNFVMPPAPTGVPILQFWPMGRKKSRVVSSWLAPDHDPANPHP
                     LWPTRLANWERILYEDLQFAPQIQESLESPGFLGMPLNYQERRIYHWHEELDRRIGLD
                     KVPQGMGVQQVLAPFLAE"
     gene            7522..8451
                     /locus_tag="VFF98_14270"
     CDS             7522..8451
                     /locus_tag="VFF98_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZU64846.1"
                     /translation="MTTIAPHQPAPSPYPDDPAQERAAAFAFAQTGPDRFRVEPVPSG
                     LLRLYGGSMIAQGLDAARLTVAAPKQAHSLHACFAQPGFTDVPLDFEVTRETDGRSFA
                     QRRVRVTQEGRLVLNVLASFQAPEESPGHHAPMPQVPPPEACPPLWDMVRAQAHRLPE
                     RHRPFWTRRQQVDWRPAEPYSLWDHAPTPLPARRHFWLRFYDAIEGGPHVHQRLLAYA
                     SDLHIFHTGLGPMGIGVMNDRLQTSSLDHAIWFHADFRVDEWLLYALECPAASSSRML
                     GRGEVFTQDGRLVASVAQQGLARLLPEARLGRI"
     gene            8448..8939
                     /locus_tag="VFF98_14275"
     CDS             8448..8939
                     /locus_tag="VFF98_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZU64847.1"
                     /translation="MSARPPRSFEDLAVGEYRQSAPRRVSREEILAFARQWDPQWFHA
                     DPEAAQGSVFGEVVASGIHVLALWRQMDHEINGDIDFVCGVGWDELRLKRAVRAGDAI
                     HVTSRIEALRPSRSGQPRGTAITRYAVVLSDGPLAGTECVSFLSTNLVYTREGRQGSG
                     CEG"
     gene            complement(9100..10260)
                     /locus_tag="VFF98_14280"
     CDS             complement(9100..10260)
                     /locus_tag="VFF98_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64848.1"
                     /translation="MDFSLTADQRQLQEAARTFARAELPAIAAACEAGNTPPPRALVK
                     RLAELGFLGINVSAELGGLGLGNIEALVVLEEFGKISSAVGFPVFESAVGPVKAIEHF
                     GSEALKRRIVPAVCAGDMVVAVSMSEPDAGSALTDLKTRGEIRGDQVVLNGTKRWCSG
                     GGHADAYVVYCRLSEAAGARAIGAVLVEKGTPGLSFGPNEELMGFRGVPSADLYFDNC
                     TVPAENIIVPAGGFRQLMEAFDLERCGNATMALAQASGALEDVCAYVEERRQFGKPIA
                     EFQAVQLKLAEMHMKVEAARLLIWRAASLAEDGLPSVLHSSTGKCFANTIAREVAGDA
                     LQLMGAYGYSKQFPMERRLRDSWGWGIAGGAIDIQKVNIAGAMLGRRFDQRR"
     gene            complement(10274..11635)
                     /locus_tag="VFF98_14285"
     CDS             complement(10274..11635)
                     /locus_tag="VFF98_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU64849.1"
                     /translation="MTVTWDTARMDVRRVVEVPHVAPEDCPPGGTPVEKAPDPDLGTA
                     LIPAARYHAPEFAALEWQRMWTKVWLLGGPAADIPAPGDYICTDIGKESVLIVRQADG
                     SVRAFPNLCLHRGNRLRPEGLGNAAEFRCMYHHWTYGLDGAITRLPDLCTFTQGGPDG
                     MALPSLPCAEWNGFLWFSLDPQVEPFADYIAPLQQHLEPYQPQRMAMVRDVTVEWDCN
                     WKASVDAFNETYHVQGIHPQLQWYLDDVNVQIDCYERHNRYLIPFAAISPRVAMPTQI
                     PPAIHEIMVKAGMDPAAYEGSVGDIRLAVQRFKRAHGAAQGKDYSRLNDDQLTDDYHY
                     LIFPNVALNVHADDILMFRQRPHASDPNRMLYDIWVFELVPDGAQWPARPKHRHFRHG
                     DRSIGQVLDQDAFNLPTVQKGLQSDFFPGLWIGEQELRIRHFHKVLDDYLYGPGGKRP
                     GDL"
     gene            complement(11653..12414)
                     /locus_tag="VFF98_14290"
     CDS             complement(11653..12414)
                     /locus_tag="VFF98_14290"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64850.1"
                     /translation="MDLGLQGKRAIIVGGARGIGYCVAEILAREGADVAIGARGEDSV
                     KDAVASLARYGTKIVGKPVNVKRAEDYKAWFAWAIEALGGVDILVPLASAGGGMGSEQ
                     YWQNAFDTDVMGPVRAVEAVLPAMSAQGKGSIVLIATTSVLEAMGGPQPYNAMKASLV
                     TWGKQLALHHGREGIRVNVVSPGPIEFEGGNWEMIKGTMEKFYQAQLRQQPQGRLGRP
                     EEVARAIVFLASEAASWCNGSHLVVDGGFTNRTHF"
     gene            12590..13252
                     /locus_tag="VFF98_14295"
     CDS             12590..13252
                     /locus_tag="VFF98_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU64851.1"
                     /translation="MARHSALLPHPPARPAAPRRPDTAQAQKSAATRTRLIEATIACL
                     VRHGYARTTTPLVAAEAGLSRGAMLHHFDTVQALIHATIVELHEKRLRAFRRASESHA
                     GNSPGMIRAYWQQLQKPGFIAFHELALAARCDADLAAILLPLQAEYQQRQQELAARLY
                     PEWQAVPGRLALAMALSQTLMEGMATALLTGALEAALVPALLAELETRLDGMRPAPEA
                     AA"
     gene            13267..>14382
                     /locus_tag="VFF98_14300"
     CDS             13267..>14382
                     /locus_tag="VFF98_14300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZU64852.1"
                     /translation="MASAKQGPLAGVKVLDLTSVLMGPYATQIFADLGADVIKVEGPA
                     GDTTRFIPPGPDPARGGMFLNVNRGKRSLALDLKQPQARAALLRLAAGADVFIHSMRA
                     GAMARLGLDYPALQAANPRILYANLYGYGRAGPYADHPAYDDIVQAASGIADLQGRLS
                     GAEPAYAATVIADKVAGLTGTYAVIAALYAREKTGLGQEIEVPMFETLVSFALTEHLC
                     GSLFVPPQGPPEYPRATAPTRRPYRTADGHIAVMIYNDKHWRAFFHALGDPEWSRDPI
                     FASMRSRTQNIALVLARVAEVLATRTTAEWLDTLRAAEIPAGPVASLADVLDDPHLAA
                     TGFWQERDSDLGRLRFPGIPTRFSATPGAIGGPGPALG"
BASE COUNT         2272 a         5158 c         4635 g         2317 t
ORIGIN      
        1 aagcgcgccg ctgaaggcgc gcatcgcccg ccattatgcc gaaaccgccg gcgtggaagt
       61 ggcgccggag cggatcatcc tcacctgcgg cgcctcgccg gcgctggtgc tggcgctggc
      121 cagcgccttt gccccgggcg atcaggtggc gctggcgcgg ccgggctatg tggcctatcg
      181 caacaccttg cgcgcgctgc acatggtgcc ggtggagatc ggctgcgggc cggaaacgcg
      241 ctatcagctg agcgctgcgc aaatcgccgc gctggatccg gcgccggccg gcctcatcct
      301 cgccagcccc gccaacccca ccggcaccat cctgccgccg gcggaactgg ccgcgattgc
      361 cgccgtgtgc gccgcgcggg gcatccgcat cgtctccgac gagatctacc acggcctctc
      421 ctttggcctg cccacccaga cggtgctggc gcatgccccc gatgccctgg tcgtcaacag
      481 cttctccaaa tatttcagca tgccgggctg gcggctgggc tggctggtgg cgcctgcgga
      541 tctggcccag gccgcctatg cgcgcatgag caacctcttc ctcacgccgc ccgtgctcag
      601 ccagcatgcc gggcttgccg cctttgacgg ccaggcagag ctggagggcc atatcgccac
      661 ctatgccgcc aaccgggcgc tgatgctggc cgccttgccg cgcctgcagc tgtccaccat
      721 cgccccgccc gatggcgcct tctacatctg ggccgatatt gcccatctgc tggcgccggg
      781 cgagaacagc atggattttg ccatcgccct gctgcgcgaa accggcgttg ccacggcccc
      841 cggcctcgat ttcgatccgg tggaaggcgg ccatttcctg cgcttcagct ttgccgtggc
      901 aacgccgctg gtggcagagg cgatcgagcg gatggtgccc ttcttcgccg cgcgggcaag
      961 gcctgagggc gccccccttt gaccttggcc aaagcgccaa aaccgctagt gtgacaggcc
     1021 gcaagccata gggggacgaa atggcagggc acggatgggt atgggccgcg ggccttgctg
     1081 gcctcgcggc gctgggcggc gcgctggcct gggcctggcg cccgctgccg gtggaagtgg
     1141 cccggcccac gcgcgggccg gcggccgatc tcgtctatgc caccggctat gtggaagcgg
     1201 cagagccggt gctggtggtg gcgcgcctca cagcgccggt gctcaaggtg ctggtggccg
     1261 agggccaggc tgtgcgcaag ggccagccgc tggccatcct cgatgccggc gagcagcgcg
     1321 ccctggccgc gcagaccgcc gcgcaggaac gcgccgccac ccttgccgaa gggcgcagcc
     1381 ttgccctctt tggcgatggc tgggtgacga aagccgcccg cgatgccgcc gttgccgatg
     1441 ccgatgccgc ccgcgccgcc catgccgccg ctgccgcccg gatcgaccag ttggtggtgc
     1501 gcgccgggat cgacggcatc gtcaccaagc gcgacatcta tcccggcgag ctggccagcc
     1561 ccagccgcac cctgttccag ctgggcgatc cggcccgcca gcgcatcacc gccacgctgg
     1621 acgagcgcga tatcgcccgc atcgcccccg gccagcccgc gctgatgagc agcgatgcct
     1681 ggcccggccg cgtgctgcac gcccatgtgg ccgaaatcac cccgggcgga gaccccacca
     1741 cccgcgcctt tcgcgtgcgc ctgcaagccg atgaacacac cgtgctgccc atggggctga
     1801 cgctggaagt gaacatcatc gcccgccagc acccccatgc cctgctgctg ccggcatcgg
     1861 cggtgagcgg cgggcgcgtg tgggtggtgg cgcagggccg cgcccgcccg cgcgccatca
     1921 ccaccggcat tgccggggcc gacagggtgg agatcaccgg cggccttggc gatggcgacg
     1981 aggtgatcct ccatcccccc gccagccttg ccgatggcac gcgggtggcg ccgcagggcc
     2041 ctgtgcatta agccatgctg gcccgcgcgc gccttctcct ccacatcgcg ctgcgccatc
     2101 tgctggtgcg ccagcggcaa acgctggtgg ccaccagcgg cgtggcggtg ggcgtggggt
     2161 ttttcctggc ggtgtcggcg atgatgatcg gcagccaggc cgatttcatc cgcacgctga
     2221 tcgatgccgc cccccacatc accctttccg acgaattgcg cagcccgcgc ccgcagccgg
     2281 gcgccattgc ctttgccggc ggcgcggtgg cgctgcatgg ctacaagctg cgcaacgagg
     2341 tgcgcgggct gaaagactgg caggcgatcc tggccgacat caacgccacg cccggcgcca
     2401 tcggctcgcc cagcctggcg ggcgctgtca ccctgcggct gggcgggcgg gaggaaccac
     2461 tggaagtggt gggggtggag ccggccatcg aaacccgcgt ctccaccatt gccgacaagc
     2521 tgcgcgccgg ccggctggac gatctcgaac gggtgcaggg cggcctcatc atcggcgagg
     2581 aaatggccgg ccggctgggc ctcaacatgg gcgatatcat cgcggccacc tcggccgggg
     2641 gcaccacccg gtcgctgcgc atcgttggcc tggtgaaaaa gggcaacacc cagatcggcg
     2701 acagcgccgg ctacatgctg ctgcgcgagg cgcaatcgct gctgggccgg cccttcatca
     2761 tcaaccgcat cggcgtgcat ctggccgatc cctatgccgc gcaggccatc gccgcccggc
     2821 tggaagcgca atatggctac aaggcgcaaa gctggcagga acgctccgcc gatttcctgg
     2881 cgctgctcgt cacccgcaac atcatcatgt atacggtcgt ctcggcgatc ctgctggtgg
     2941 cgagctttgg catatacacc gccgtctcga acagcgtggc cgacaagaag cgcgatatcg
     3001 ccatcctgcg ctccatgggc ttttccagcg gcgacctgca ggcgatcttc gtgatcgagg
     3061 ggctgatgct ggcgctggtg ggggtggcgc tgggctgggc gctgggcacc gggctgatgg
     3121 atgtgctggg cgcgctcacc ttttccatcg gcggcgatcc gcagaagatc ccgctcgacc
     3181 ggtcgggccg gcaatatgcc gttgccgcgg ccgcctcgct gctggccggc acgctggccg
     3241 cctggctgcc cgcccgcaag acagcgcggc tggatccggt ggatatcctg cggggcgcgg
     3301 tatgagcctg ctgctggaag cgcgcggcct gtcgcgccag ttgccgggcg atccgccggt
     3361 gcggctggtg gaggaagtga acctggccat ccgccccggc gctttcaccg ccattgtcgg
     3421 cccctcgggc tgcggcaaaa gctcgctgct ctatctcctc ggcctgatcg accggccctc
     3481 cagcggccag ttgctgctgg aaggcgtgga tgtggccccg ctttccggcg atgagcgcgc
     3541 ccgactgcgg ctggagcgtt tcggctttgt cttccagttc catttcctgc tgccggaatt
     3601 ttccgcgctg gaaaatgtgc tgctgccgat ccgccggctg ggccgcctgg ccggccctgc
     3661 cgccaccgcc cgcgccatgg ccctgctgga aggcgtgggg ctgggcgaga aggccggcaa
     3721 gacgcccgag aaactgtctg gcggggagcg ccagcgggtg gcgatcgccc gcgcgctggc
     3781 caatgatccg gcgctgatcc tgggcgacga gcccaccggc aacctcgatt cggccaacag
     3841 cgcgcgggtg gtggaaatgc tgcgcggcct ggcccatggc gaggcgcgcg cggtggtctg
     3901 cgtgacgcat gacccgcgca tcgccgagat cgccgatgtg cgggtggaaa tgctggacgg
     3961 gcgcgtggtg gcgatagagg agaattagcg cgggccgggc gacagggccc cggcgccaag
     4021 ccccgcccgg tgctatcggg cgggggcttt tgcgcaacgc ccgcgggtta cgccggctgg
     4081 cccaccttca ggctctccag cgtcgagata tcctcgcgca ggcgcaccag cgtcttgccg
     4141 gtggtggtgc cgctcatcag gcggatgaag gcctcgggcg tgtgctccag cccggtcgtc
     4201 acgttttcgg tggcgatcag ctggcccttg cgcacccagt cggccagcgc atcttccgcc
     4261 tcatgcagca tgtcggccgc ctcataggca aggaagccgc gcatggtgat gcggttcacc
     4321 agcacctgcc agatgttgcg cagcagatag ggctcgttct ggtggttgta gttcgagatc
     4381 atgccgcaca ccggaatgcg gccaaaggcc ttcatctgcg gcagcacggc atccagcgtc
     4441 tcgccgccga cattgtcgaa atagagatcg atgccattgg ggaaggccgc gcggatcgcc
     4501 tcggccagat cgggggcggc gcgatagtcg atcacatcgt caaagcccag cacctcgcgg
     4561 gcgatgcgcg ccttgtcggc gccgcccacc gtgcccaccg cccggcagcc cgacaggcgc
     4621 gccacctgcc ctgccaccga acccacagcc cctgccgccg cgctgatcag cacctcgtcg
     4681 cccgcgcgca tctcgccata gcgcttgagg ccgacatagg cggtgatgcc cgaccagccc
     4741 agcgcgccca tataggcggt cagcggcatg tcgggggcga tgcgcacgtt ctcgaagccg
     4801 accgcgcccg gcgtcagcac cgaaaattcc tcccagccga tgaaggcgcg gccataatcg
     4861 ccctcgtgga aatcgggatt gcgcgaggcc accacccggc ccagcaccat ggtgggaatg
     4921 agcccgccaa tcggcaccgg cggcatatag ctcctcacat catccagcat cccgcgcacc
     4981 gccggatcga tggcgaagac gaggttctcg atcagcacct ggccttcggc cagttcgggc
     5041 acgtccactt cttccaggcg gaaattggca ggcaccggca cgcccgaggg gcgcgaggca
     5101 agcacgatac ggcggttctt catggggcgt gtccgaagct ggagggaaag gcgcggctca
     5161 gagcggcacg gcgcgcgggt tggccgcgtc atacacctgc gcggtcatgc cggtgatgtg
     5221 cggcgagtag cggttgaaga aggtggtgat ccacggcatc gtcagatgca ccttcagatg
     5281 ctcctcgctg tcccagatct ccagcacgtt gatctcgccc ttggcggcat cttccaccag
     5341 cagcgaataa tggcggcagc cctcttcttc cagcaccttg gggcgcatct cggcgacatc
     5401 ggcggcaaag gcatcgagct gcgccgggtc gatcgacagg gtgcctgcaa caaagatcat
     5461 catcgctctc ctggtaggca tggtggcccg gggcctggcc atgcatgcgg gtgggggcgc
     5521 cagcttgtac cggcgccttg ccgcttgtca tgccgcttcc ttccacaatc cggcggcagg
     5581 cgagactagc ctattccaca ggtcgtccag tctgccatca gctggcccac cattccgcca
     5641 tcgccccgcg cagggccgca gagaccagct ccaccaccgg cccgccatcg ggcggcgcca
     5701 gataggcgat cagcccataa ccgctggccg ccggcgcgcc cgagagcacc atgcgccagc
     5761 ccgccgccag cagcgcctct gcctcgcccg ccagatcctc cacccacacg cccagatgcg
     5821 cctggttttc cgcccgcagc gcgccatagg catcaccggg ccgcgcctcg atcagttcca
     5881 ggtggatcgg cccctggcgc gaataggcga ccttcagatg cgccaccgcc tccgtgccat
     5941 cggccagcca catcggcatg ggggaaaagc tgcgcaccgg cgcccattcc acccccagcg
     6001 ccgggcccag cgcggccatg gtggcggcca gatccgccac cagcaggccg tggtgataga
     6061 tatcggccag cgccgctgta gctggctcgt tgctcttgct catcgcactg ctcctcttcc
     6121 tgcgctccat ggtaaaccac gcatggccgc cgataacctc tccattcggc tgatttgcgg
     6181 ccataatccg cggcccttga caggccggcc cgaatataaa acaatccgac atgtctgttt
     6241 ggaaaattgg accaccaaag gcctgattcc accagccaag ggagtatgtc tgatgccgag
     6301 cccggccgct gccgaactgt gcccgccgcc tgccgacgat ctggcccgca tggcctggga
     6361 acgcgcccgc aaggccccgc cggaaggctt ccccgccctg cccgacatcc ccggccagcg
     6421 ctatgtgaac gaggccttcc tctcgctgga agagcaacgg atgtggcagc gcgcctggct
     6481 gtatgccggc cacaccgacc agctgcccac ccccggctcc tggttcctca cccgcaacac
     6541 cggctctccg gtgatcgtgg cgcgcacgct ttcgggcgag atccgtgcct attacaacac
     6601 ctgccgccac cgcggcgcgc cgctcgttac cgaagcggcg ggcgaggcgc gcggcttcat
     6661 gtgcagctat cacggctgga gctacacgct ggaaggccgc ctcaccgcca tccgcgatgc
     6721 gcgcgatttc gtcgatttcg atctggcctg ccacagcctg gtgccggtgc gctgcgaatt
     6781 gctgggttcg ctgatcttcc tcaaccagga tgcccaggcc caaccgctgc tcgaccatat
     6841 cggcccgatg gccaaggagc tggagcaatt gcagctcgac aagctgcgcc ttgtcgacag
     6901 ccgctcctat gatgtcgatt gcaacgtgaa ggtgctgctc gatgccttcc tcgaggtcta
     6961 tcacctgaag tcgatccatg tgaacacggt ggaccgcttc ctcgatcacc gggcgatgat
     7021 cgtcacgctc tggcccaatg gccacagccg catggccacc cccaaccggc gcgagggctg
     7081 gaccgaccct ggcaccgtgg gcatgccgga aattcccacg gtttcggaat ttctggcgaa
     7141 gaacaacgtc tcctatgcgc tctatcccaa tttcgtgatg ccgccggcgc ccaccggcgt
     7201 gccgatcctg cagttctggc cgatgggccg caagaaaagc cgcgtcgtct ccagctggct
     7261 ggcgcccgat catgatccgg ccaaccccca tccgctgtgg cccacccgcc tcgccaattg
     7321 ggaacgcatt ctctacgagg acctgcaatt tgccccgcag atccaggaat cgctggaaag
     7381 cccggggttc ctgggcatgc cgctcaatta tcaggagcgg cgcatctatc actggcacga
     7441 agagctggac cggcgcatcg gcctcgataa agtgccccaa ggcatgggcg tgcaacaggt
     7501 gctggcgccc ttccttgcgg agtgacgacg atagcgcccc accagcctgc cccctcgccc
     7561 taccctgatg atccggcgca agagcgcgca gccgccttcg cctttgccca aaccgggcca
     7621 gaccgcttcc gggtggagcc ggtgcccagc gggctgctgc ggctctatgg cggcagcatg
     7681 atcgcccagg ggctggatgc cgcccgcctt accgtggccg cgcccaagca ggcccacagc
     7741 ctgcacgcct gcttcgccca gcccggcttc accgatgtgc cgctggattt cgaggtcacg
     7801 cgcgagaccg acgggcgcag ctttgcccag cgccgcgtgc gcgtaacgca ggaggggcgg
     7861 ctggtgctca atgtgctggc ctccttccag gcgccggagg aaagccccgg ccaccacgcc
     7921 cccatgccgc aagtgccccc gcccgaggcc tgcccgccgc tgtgggacat ggtgcgcgcc
     7981 caggcccacc gcctgcccga acgccaccgc cccttctgga cccgccgcca gcaggtggac
     8041 tggcgccccg ccgagcccta cagcctgtgg gaccacgccc ccaccccgct gcccgcccgg
     8101 cggcacttct ggttgcgctt ctacgatgcg atcgagggcg gcccccatgt ccaccagcgg
     8161 ctgctggcct atgcgtcgga tctgcacatc ttccacaccg gccttgggcc catgggcatc
     8221 ggcgtgatga acgaccggct gcaaacctcc agcctcgatc atgccatctg gttccacgcc
     8281 gatttccggg tggatgaatg gctgctctac gcgctggaat gcccggccgc ctcttcctcg
     8341 cgcatgctgg ggcggggcga ggtgttcacg caggatggcc gactggtggc cagcgttgcc
     8401 cagcagggcc tggcccgcct gctgcccgag gcccggctgg gccggatatg agcgcccgcc
     8461 cgccccgcag cttcgaggat ctggccgttg gcgaatatcg ccagtccgcc ccgcggcgcg
     8521 tcagccgcga ggagatcctc gcctttgccc gccagtggga tccgcaatgg ttccacgccg
     8581 accccgaggc cgcgcaaggc tcggtgtttg gcgaagtagt ggccagcggt atccatgtgc
     8641 tggccctgtg gcgccagatg gaccacgaga tcaacggcga catcgatttc gtctgcggcg
     8701 tgggctggga cgagctgcgc ctcaaacgcg ccgtgcgggc gggcgatgcc atccatgtca
     8761 ccagccggat cgaggcgctg cgcccctcgc gcagcggcca gccgcgcggc acggcgatca
     8821 cccgctatgc ggtggtgctg tccgatggcc cgctggccgg caccgaatgc gtgagcttcc
     8881 tcagcaccaa tctcgtttac acgcgggaag ggaggcaagg gagtggatgc gagggttaac
     8941 accctcgcgc tcccattctt tggggccaag gcgccagcct gccccgccgg aggcatgaat
     9001 gcatgcggct gcgccgcggg cgcttcatcg aaagcgggcg cgacaataag gtaatgggag
     9061 cgcgagggtg ttaaccctcg catccccccc ctttgcccct caccgccgct gatcgaaccg
     9121 ccgccccagc atggcgccgg cgatattcac cttctggata tcgatcgccc cgccggcaat
     9181 gccccagccc cagctgtcgc gcaggcggcg ttccatgggg aactgcttgg aatagccata
     9241 ggcgcccatc agctgcagcg catcgccggc cacttcgcgg gcgatggtgt tggcaaagca
     9301 tttgccggtg gaggaatgca gcacggaagg cagcccgtcc tcggccagcg aggcggcgcg
     9361 ccagatcagc aggcgggcgg cttccacctt catgtgcatc tcggccagct tcagctgcac
     9421 cgcctggaat tcggcgatgg gcttgccgaa ctggcgccgc tcctcgacat aggcgcacac
     9481 atcctccagc gcgcccgagg cctgggccag cgccatggtg gcattgccgc agcgttccag
     9541 atcaaacgcc tccatcagct ggcgaaagcc gccggccggc acgatgatgt tttccgccgg
     9601 cacggtgcaa ttgtcgaaat agagatcggc cgagggtacg ccgcgaaagc ccatcaactc
     9661 ctcgttgggg ccaaagctga ggccgggcgt gcccttttcc accagcaccg cgccgatcgc
     9721 cctggccccc gccgcttccg acaggcggca atagacgaca taggcatcgg catgcccgcc
     9781 gcccgaacac cagcgcttgg tgccattcag caccacctga tcgccgcgga tctcgccgcg
     9841 ggttttgagg tcggtaaggg cgctgccggc atccggttcc gacatcgaca ccgccaccac
     9901 catatcgccc gcgcacaccg ccggcacgat ccgccgcttc agcgcctcgg agccgaaatg
     9961 ctcgatcgcc ttgaccggcc ccaccgcgct ttcgaacacc ggaaagccca ccgccgagga
    10021 gatcttgcca aattcctcca gcaccaccag cgcctcgata ttgcccagcc ccagcccgcc
    10081 cagctctgcc gacacgttga tgcccagaaa gcccagctct gccaatcgct tcaccagcgc
    10141 gcggggcggc ggcgtgttgc cggcctcgca agcggcggca atggcgggca gttcggcgcg
    10201 ggcgaaggtg cgggcggctt cctgcagctg gcgctggtcg gcggtgaggg aaaaatccat
    10261 ggggcagcag ctttcacaga tcgcccgggc gcttgccgcc ggggccatag agataatcgt
    10321 cgagcacctt gtggaaatgg cggatgcgca gttcctgctc gccaatccac aggccgggga
    10381 agaaatcgga ttgcagccct ttctgcaccg tcggcaggtt gaaggcatcc tgatcgagca
    10441 cctggccgat gctgcgatcg ccatgcctga agtggcggtg tttgggccgc gccggccatt
    10501 gggcgccatc gggcaccagt tcgaacaccc agatgtcata cagcatgcgg ttgggatcgc
    10561 tggcatgggg gcgctggcgg aacatcagaa tgtcgtcggc atggacgttg agggcgacat
    10621 tggggaagat cagatagtga tagtcatccg tcagctgatc atcgttcagc cggctatagt
    10681 ccttgccttg cgccgcgcca tgggcgcgct tgaagcgctg cacggccagg cggatatcgc
    10741 ccacgctgcc ctcataggcg gccggatcca tgccggcctt caccatgatc tcgtggatgg
    10801 cggggggaat ttgcgtgggc atggccaccc gcggcgaaat ggcggcaaag gggatgagat
    10861 agcggttgtg ccgctcgtag cagtcgatct gcacgttcac atcatcgaga taccattgca
    10921 gctgcgggtg gatgccctgc acatgatagg tctcgttgaa ggcatcgaca gaggccttcc
    10981 agttgcaatc ccattccacc gtcacatcgc gcaccatcgc catgcgctgc ggctgatagg
    11041 gctccagatg ctgctgcagg ggggcgatgt aatcggcaaa gggctccacc tgcggatcga
    11101 gcgagaacca caggaagccg ttccattccg cgcaaggcag gctgggcagc gccatgccat
    11161 cggggccccc ttgcgtgaag gtgcacagat ccggcaggcg ggtgatggcg ccatccagcc
    11221 cataggtcca gtggtgatac atgcagcgaa attccgccgc attgcccagc ccttcaggcc
    11281 gcagccggtt gccccggtgc aggcacagat tggggaaggc ccgcaccgag ccatccgcct
    11341 gccgcacgat cagcacgctt tccttgccga tgtcggtgca gatgtaatcg cccggcgccg
    11401 gtatatcggc cgcggggccg cccagcagcc acactttcgt ccacatccgc tgccattcca
    11461 gcgcggcaaa ttccggcgcg tgatagcgcg ccgccgggat aagcgccgtg cccagatccg
    11521 gatcgggcgc cttttccacc ggcgtgccgc cgggcgggca atcttccggc gccacatgcg
    11581 gcacctccac cacccggcgc acatccatgc gggctgtatc ccaggtcacg gtcatcgccc
    11641 gcgcctcccc tgtcagaaat gggtgcggtt ggtgaagccg ccatccacca ccagatgcga
    11701 tccattgcac cagctggccg cctcgctggc caggaagacg atggcgcgcg ccacttcctc
    11761 gggccggccc aggcgcccct gcggctgctg gcgcagctgg gcctgataga atttctccat
    11821 cgtgcccttg atcatctccc aattgccgcc ctcgaactcg atcggcccgg gcgagacgac
    11881 attgacgcgg atgccctccc ggccatggtg cagcgccagc tgcttgcccc aggtgacgag
    11941 gctggccttc atcgcattat agggctgggg cccgcccatc gcttccagca cggaagtggt
    12001 ggcgatcagc acgatgctgc ccttgccctg cgcgctcatc gccggcagca cggcttccac
    12061 cgcgcgcacc ggacccatca catcggtgtc gaaggcgttc tgccaatatt gctcggaccc
    12121 catgccgccg ccggccgaag ccagcggcac aaggatatcc accccgccca gcgcctcgat
    12181 cgcccaggcg aaccacgcct tgtagtcctc cgcccgcttc acattcacag gcttgccgac
    12241 gatcttcgtg ccatagcggg caaggctggc cacggcatcc ttcacgctgt cttcgccgcg
    12301 cgcgccaatc gccacatcgg cgccctcgcg cgccaggatt tcggccacgc aatagccaat
    12361 gccgcgcgcc ccgccgacga tgatcgcccg cttgccctgc aaacccagat ccatggctgg
    12421 ttcttcctct gcccggtggg gcggctggtg cgcccctgct gtttgcggcc agcttaggca
    12481 ccgcgtcgcc gattgcaata aaaaacatgc ttgattgcat tttttaacgg gcgcatgaag
    12541 gcatggccca gcgcccgtga agcggcgcgg cgcaggagag aacacagccg tggcgcgcca
    12601 ttcagccctt ttgccccatc cccctgcccg gcccgccgcc ccccgccggc cagataccgc
    12661 ccaggcccag aaaagcgccg ccacccgcac ccggctgatc gaggcgacca tcgcctgcct
    12721 ggtgcgccac ggctatgccc gcaccaccac gccgctggtg gcggccgagg caggcctctc
    12781 acgcggggcc atgctgcatc atttcgacac cgtgcaggcg ctgatccacg ccaccatcgt
    12841 ggaactgcac gaaaagcgcc tgcgcgcctt ccgtcgcgcc tccgaaagcc atgccggcaa
    12901 cagccccggg atgatccgcg cctattggca gcaattgcag aagcccggct tcatcgcctt
    12961 tcacgaactg gcgctggcgg cgcgctgcga tgccgatctg gcggcgatat tgctgcccct
    13021 gcaggccgaa taccagcagc gccagcagga actggccgcc cgcctctatc ccgaatggca
    13081 ggccgtgccc gggcgcctgg cgctggcgat ggcgctatcg caaacgctga tggaaggcat
    13141 ggccaccgcc ctgttgaccg gcgcgctgga ggcagcgctg gtgccggccc tgctggccga
    13201 actggaaacc cggctggacg gcatgcgccc ggcccccgaa gccgccgcct gaaggagaga
    13261 acaagaatgg caagcgcgaa acagggcccg ctggccggcg tgaaggtgct ggatctgacc
    13321 agcgtgctga tgggccccta tgccacgcag atcttcgccg atctgggcgc ggatgtgatc
    13381 aaggtggaag ggcccgcggg cgataccacc cgcttcatcc cccccgggcc agacccggcg
    13441 cgcggcggca tgttcctcaa cgtcaaccgc ggcaagcgca gcctggcgct cgatctgaag
    13501 cagccgcagg cgcgcgcggc gctgctgcgg ctggcggcgg gggcggatgt gttcatccat
    13561 tccatgcggg cgggcgccat ggcccggctg gggctggatt atccggcgct gcaggcggcc
    13621 aatccgcgca tcctctatgc caatctctat ggctatggcc gggccgggcc ctatgccgat
    13681 caccccgcct atgacgacat cgtgcaggcc gccagcggca ttgccgatct gcaggggcgg
    13741 ctgtcgggcg cagagccggc ctatgccgcc accgtcatcg ccgacaaggt ggccgggctg
    13801 accggcacct atgcggtgat cgctgccctc tatgcccgcg agaagaccgg gctgggccag
    13861 gagatcgaag tgccgatgtt cgagacgctg gtctccttcg cccttaccga gcatctgtgc
    13921 ggcagccttt tcgtgccgcc gcaaggcccg cccgaatatc cgcgcgccac cgcccccacg
    13981 cgccgcccct atcgcaccgc cgacgggcat atcgccgtga tgatctacaa cgacaagcac
    14041 tggcgcgcct tcttccacgc gctgggcgat ccggaatggt cgcgcgatcc gatcttcgcc
    14101 tccatgcgca gccgcaccca gaacatcgcc ctggtgctgg cccgggtggc cgaggtgctg
    14161 gccacgcgca ccaccgctga atggctggac accttgcgcg cggccgaaat ccccgccggc
    14221 cccgtggcca gcctggccga tgtgctggac gatccgcatc tggccgccac cggcttctgg
    14281 caggagcgcg acagcgatct gggccgcctg cgctttcccg gcatacccac gcgcttttca
    14341 gcgacgcccg gcgccattgg cgggcccggc ccggcgctgg gg
//
LOCUS       DASRYE010000278        10573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_260369,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000278 DASRYE010000000
VERSION     DASRYE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260369"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFF98_14305"
     CDS             <1..534
                     /locus_tag="VFF98_14305"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZU64853.1"
                     /translation="EAGLRAALDPVRGLDHGAWVPLMLMYPDADIPVVQLSIQAHLGP
                     GHHLQLGRALAPLRAENVLILASGSLTHNLGALRFYRGEGGEGEPDWVTDFAEWVHEA
                     IAQSRVCDLISYRALAPHAVDNHPTDEHLLPLFVALGAGLHETASPAEHERRLAARRL
                     HASSTFGVLRMDAYAFD"
     gene            577..1164
                     /locus_tag="VFF98_14310"
     CDS             577..1164
                     /locus_tag="VFF98_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZU64854.1"
                     /translation="MTKILVIRSAATGEASVSNKLIDAYLAAHPGAEVVDRNLDAEPV
                     PHVTSASLAGIGRQAPEGGQFAEARALQDQLIGEVFGADVLVIGLPLYNFGMPSTLKA
                     WFDYIARAGTTFQYTEKGPEGLVKGKKAIIAQARAGKYDDAAGFPFAVAHTKALLGFI
                     GVTDVEVITAEGLAFGPDAAAAAIAEAATKIAELA"
     gene            1197..1820
                     /locus_tag="VFF98_14315"
     CDS             1197..1820
                     /locus_tag="VFF98_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZU64855.1"
                     /translation="MTFSTSALRGLVAAAAILAASPAFAQTPGNAAPTAAKAGTYGVE
                     PWHTRVQFSVDHMGFTDWFGDFTGISGQLVLDPAHAAADKVDITIPVASVSTTNTKLD
                     GELKSADWFDAARYPEIHFVSTKVVRTGPAAALITGDLTFHGVTKPVTLAAHFHGAGV
                     NPLSKGFTVGFDATTSIKRSDFGVKTYLPMIGDKVTLRISAAFEKKD"
     gene            complement(1878..3761)
                     /locus_tag="VFF98_14320"
     CDS             complement(1878..3761)
                     /locus_tag="VFF98_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZU64856.1"
                     /translation="MPPLFSLARLAAVLLPLAAVAASPPPARITSVDDFQARDAARID
                     PETAPGRALFAQHCAICHMGAVPKAPSPQFLGMLSPAAIVKALNEGVMREMATGLAPA
                     ERIAVAEYLTRTPYNPAQRAPEPPACTGANAAIDWAAGAPAIGWGRDNRRFVPAAVAG
                     LAKADVPRLKLLWAWAYPAAFRARSQPSIAWGTLFVGSQDGTIYAFDPRHGCTRWIRH
                     TGAEVRTALVADPAARRLYFGDLLGRVHAIDAATGADLWQVRPSAHADATITAAPTLA
                     GGLLYAAVSSLEVAQAGNPHYACCTFRGLVAALDPATGREVWRFYTAGEPKPQPATPD
                     GTQRFGPSGAPVWNSPTYDARRGLLYFGSGENYSSPADGNSDAIFAVEARTGRLVWRT
                     QLTKGDAWNVGCMLALESCPQENGPDYDLSASALLIDLGGGRDILVLGQKSGWAYGVA
                     PDSGAILWRRQLGHGGTQGGVHFGMAAEGTRIFAPVNDMANTYDGRHYDPALRGAGLH
                     ALDAATGRPFWFAKAADRCAGKPFCDPGISAAATAMPGVVFAGHLDGMLRAYDAASGA
                     LLWQADTTAPVTGVDGRQGHGGSMSGPGPAIWQGLVIANSGYGMYSHMAGNVLLVYAA
                     GGS"
     gene            complement(3784..4080)
                     /locus_tag="VFF98_14325"
     CDS             complement(3784..4080)
                     /locus_tag="VFF98_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZU64857.1"
                     /translation="MPARSFIAQLRCRPEARADLICLQAELKRLVAEHEPDCLVYELF
                     QSEADPDLFQVVATFRNEAAFEAHMHIDFHDRLVPPILATLAEDMQLAFYRSLA"
     gene            complement(4070..4384)
                     /locus_tag="VFF98_14330"
     CDS             complement(4070..4384)
                     /locus_tag="VFF98_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZU64858.1"
                     /translation="MAFSFLSRFRIRPEQEAAFITLARQMEALSAEEPGTLHYQFFRL
                     AEPGLFAVYESFVDAAADKAHIAAAANQPLIARMIACMDGPYEREMLYDLAPAAETAD
                     AR"
     gene            4778..7264
                     /locus_tag="VFF98_14335"
     CDS             4778..7264
                     /locus_tag="VFF98_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64859.1"
                     /translation="MNTKDLWRGTAMAAVAMGAVAACSPAVAAGADAAGTAAGTSADT
                     PAGLNEIVVTATRRDTNLQRTPIAVSAVDTNLIRTASPRDVGDLAAFVPNFSAGTITN
                     FNAASFAMRGVGQTSIIVYFEPPVAVLVDDFVVSSVQTQLLDTFDIAQVEVLRGPQGT
                     LFGKNTTGGAVTVKTKKPELGILGVDAHAEYGSFNTGKFQLAFNLPIGDTIAVRLVGG
                     MEKSDGYYKNGACYGPVTGIVTTNKFEGASGCLDGKSAGGKDVAQARAKILWQPSDRF
                     KLLAQYEWLRDRSQTMPSVNENASYTGTGSFLTSTLGVINTNGASSDPLNNAAVSYRQ
                     DGLIKMRDGQRISVDGFYLNADYKLGFGTLTSVTGWRYQRSRLPNSYAGAVAIASDGQ
                     PLSFFDATRDDNRSTFQQELRFATNFGGRFDMVVGGFYQHENIDFCVAQLLGFLDLTS
                     GPIAGLGNWNDNPYILCNAQTSRSRAVFAEGTYKITPKLSLTAGGRYTWENKTWWGRQ
                     QVFIPQLNGGWDTSINIQNALDANVFAYPAGVISLTDSAHEPTYRASLGWQAQRDLYF
                     YATYSHGFKAGAFNDQIGGFAAFGSDYNAFRTAAAATKPEKADSYEIGVKSEFLNHRV
                     RFNLTGFWVEYKDLQKQLNVPIEVNGAPNQVTIFVNAASATVKGIEAEAAARLFQGFT
                     LRAVLGYQDGHYNQYTAPSAGYNLASAPLDRTPRWQWTLDGTYERPLNRQFKWAIDAS
                     AAYTGSNLNTQSITDPAGNTFLNARTLVNASLTLSQIHDDYYIKVVGRNLTDERYRTA
                     SQNVAGLWLNSNYGPPRYVGLELGAKFRGF"
     gene            7319..8668
                     /locus_tag="VFF98_14340"
     CDS             7319..8668
                     /locus_tag="VFF98_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64860.1"
                     /translation="MRLDFDEWNIHVGPDHPFNRRAAPIDNGTARPAPDRYTSPAFAR
                     AEWERVFARSWLLAGPAADIPEPGDFMRFDIGGESFLIVRGEEGQIHAHYNVCPHRGS
                     RLVTEDFGSVAQFSCPFHSWKFALDGRCAAVTDAATFRPEVLAHGTQLSSVRCEVAVG
                     LVFIAMDPAIRPLAEWLAPILPQLALYDIDRMYVIQHRQSAWGANWKGGVDAFAEIYH
                     LHAVHPQTQGLMDDRTQIDLWPGGLSRQFVPFAQPSPRYADQEGVNPGIQLMLRDAGI
                     DPAAYTGTARETRAAVQAAKRRRSAELGLGYEKFSDSQLSDSVVYSLFPNVQIGCHPE
                     AVFLHRFLPHASDPARFTYDTCILYRHIDAPGYSAPAWMGLGDEVDFSGRTRPAITRT
                     GPGEPPGLGEVLDQDSDLLPVVQQGAGSRGFRGPLWSEQEGRLRHFHAELDRMMAAG"
     gene            complement(8884..9642)
                     /locus_tag="VFF98_14345"
     CDS             complement(8884..9642)
                     /locus_tag="VFF98_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64861.1"
                     /translation="MGRLAGKVALVTGAGSGLGAADARAMAREGAQVILTDVALGAAE
                     AVAREIGGTAIALPLDVASEAQWIATMAAVEQRFGRLDVLVNNAGVVLNADIEATSLE
                     QFHKVNAIMIDGVFLGCKHALPLMHKSGGGSIINMSSVGAILGYPIFFAYSAAKGAVR
                     AMSKSIAVMCQEKGYKIRCNSVHPGAIETPMVQAAEGRVGQARTIPQGVLPPGTPGHP
                     DDVAALVVFLAADESRFITGAELVIDNGVTIRPF"
     gene            complement(9673..>10573)
                     /locus_tag="VFF98_14350"
     CDS             complement(9673..>10573)
                     /locus_tag="VFF98_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64862.1"
                     /translation="PPRSFRPDPEDTTRPARGLDDAALAARQSVFRTDLLAGQVALVS
                     GGGSGIGKAAALLLARLGAEVAICGRDEEKLAATARFIEGETGRAIFPLATNIRDADA
                     VEALIGRVHDRFGALHTLVNNAGGQFPQDAIDFSRKGWLAVIDTNLNGTWWMMQEAAR
                     RWRADGAAGNIINIVANVERGMPQAAHTCAARAGVIYLSKTLATEWAPHNIRINCIGP
                     GVIETEGFRMYPEEALARFHMANPMKTRGDAWDVAEAIAYLASPAARFVNGDLILLDG
                     GQAQWGVVWPAGMPDYFNEGGAA"
BASE COUNT         1769 a         3552 c         3521 g         1731 t
ORIGIN      
        1 gaggcggggc tcagggccgc gctcgatcct gtccgcgggc ttgatcatgg cgcctgggtg
       61 cccttgatgc tgatgtatcc cgatgccgat atcccggtgg tgcagctgtc cattcaggcg
      121 catctggggc cgggccatca cttgcagctg ggccgggcgc tggcgccctt gcgcgcggaa
      181 aacgtgctga tcctggccag cggcagcctc acccacaatc ttggcgcgct gcgcttctat
      241 cgcggcgagg gcggcgaggg cgagccggac tgggttaccg attttgccga atgggtgcat
      301 gaggccatcg cgcagagccg cgtgtgcgat ctcatctcct atcgggcgct ggccccccat
      361 gcggtggaca atcaccccac cgacgagcac ctgctgccgc tgttcgtggc gctgggcgcg
      421 gggctgcatg aaacggccag ccctgccgag cacgagcggc gcctggccgc ccggcgcctc
      481 catgccagca gcacctttgg cgtgctgcgc atggatgcct acgctttcga ctgatccctc
      541 ctgccaaacc cctcatccca aacaaggaac cttcccatga ccaagattct cgtgatccgt
      601 tccgccgcca ctggcgaggc ttccgtcagc aacaagctga tcgacgctta tcttgccgcc
      661 catcccggcg ccgaggtggt ggaccgcaac ctggatgcag agccggtgcc gcatgtcacc
      721 tcggccagcc ttgccggcat tggccgccag gcccccgaag gcggccagtt tgccgaagcg
      781 cgcgccctgc aggatcagct gatcggcgaa gtgtttggcg ccgacgtgct ggtgatcggc
      841 ctgccgctct acaatttcgg catgccctcc acgctgaagg cctggttcga ttatatcgcc
      901 cgcgccggca ccaccttcca gtacaccgag aagggccccg aagggctggt gaagggcaag
      961 aaggcgatca tcgcccaggc ccgcgccggc aagtatgacg atgccgccgg cttccccttc
     1021 gcggtggccc acaccaaggc gctgctgggc ttcatcggcg tgaccgatgt ggaagtgatc
     1081 accgccgagg gcctggcctt tggcccggat gccgccgctg ccgccattgc cgaggccgcc
     1141 accaagatcg ccgaactggc ctgatcggcc ccctcttgtt tccggagaac agacccatga
     1201 ccttctcgac ctcggccctg cgtggcctgg tggccgctgc cgctatcctc gctgccagcc
     1261 ccgccttcgc ccagacgcct ggcaatgccg cccccactgc cgccaaggcc ggcacctatg
     1321 gcgtggagcc ctggcacacc cgcgtgcagt tcagcgtgga ccacatgggc ttcaccgact
     1381 ggttcggcga tttcaccggc atttcgggcc agctggtgct ggacccggcc catgccgcgg
     1441 ccgacaaggt ggacatcacc atcccggtgg ccagcgtgtc gaccaccaac accaagctgg
     1501 atggcgagct gaagagcgcc gactggtttg acgccgcccg ctaccccgag atccacttcg
     1561 tctcgaccaa ggtggtgcgc accggccctg ccgctgccct catcaccggc gatctcacct
     1621 tccacggcgt gaccaagccg gtgacgctgg ccgcccattt ccacggcgcc ggtgtcaacc
     1681 cgctgagcaa gggcttcacc gtgggctttg atgccaccac cagcatcaag cgcagcgatt
     1741 tcggcgtgaa gacctacctg ccgatgatcg gcgacaaggt gaccctgcgg atcagcgcgg
     1801 ccttcgaaaa gaaggactga gcgggccacc gccggcaacg aaaagggggc gaggggccag
     1861 gcctctcgcc ccctttttca tgagccgccc gccgcataga ccagcagcac attgccggcc
     1921 atgtgcgaat acatgccata gccggaattg gcgatgacca gcccctgcca gatggccggg
     1981 cccggcccgc tcatgctgcc gccatggccc tggcggccat ccaccccggt cacgggggcg
     2041 gtggtgtcgg cctgccacaa cagggcgccg ctggccgcat cataggcgcg cagcatgcca
     2101 tcgagatggc cggcaaagac cacgccgggc atggccgtgg ccgccgccga tatgcccgga
     2161 tcgcaaaacg gcttgccggc gcaccggtcg gccgccttgg caaaccagaa cgggcggccg
     2221 gtggcggcat ccagcgcatg cagccctgcg ccgcgcagcg ccgggtcgta gtggcggccg
     2281 tcataggtgt tggccatgtc gttcaccggg gcgaagatgc gggtgccttc ggccgccatg
     2341 ccgaaatgga cgccgccctg cgtgccgcca tggcccagct gccgccgcca gagaatggcg
     2401 ccgctatcgg gcgcaacgcc ataggcccag cccgatttct gccccagcac gaggatatcc
     2461 ctgccgccgc ccagatcgat cagcagggca gaggcggaca ggtcgtagtc tggcccgttt
     2521 tcctgtgggc agctttccag cgccagcatg cagcccacgt tccaggcatc gcctttggtc
     2581 aattgcgtgc gccagacgag gcggccggtt ctggcctcca ccgcgaagat cgcgtcggaa
     2641 ttgccatcgg ccggcgagga ataattctcg cccgagccga aatagaggag gccccggcgc
     2701 gcgtcatagg tggggctgtt ccacaccggc gcgccagagg ggccgaagcg ctgggtgcca
     2761 tcgggcgtgg cgggctgagg tttgggctcg ccagcggtgt aaaagcgcca gacctcgcgg
     2821 ccggttgccg gatcgagcgc tgccaccagg ccgcggaagg tgcagcaggc ataatgcgga
     2881 ttgcccgcct gcgccacttc cagcgaggag acagcggcat agagcaggcc gccggccagc
     2941 gtgggcgcgg cggtgatggt ggcatcggca tgggccgagg ggcgcacctg ccacagatcg
     3001 gcgccggtgg cggcatcgat cgcatgcacg cggcccagca gatcgccgaa atagagccgc
     3061 cgcgcggccg gatcggccac cagcgcggtg cgcacttcgg cgccggtgtg gcggatccag
     3121 cgggtgcagc catggcgcgg atcgaaagcg tagatcgtgc catcctggct gccgacgaac
     3181 agcgtgcccc aggcgatgga aggctgcgaa cgggcgcgga aggccgccgg ataggcccag
     3241 gcccacagca gcttcaggcg cggtacatcg gccttggcaa ggccggccac ggcggccggc
     3301 acgaagcggc ggttgtcgcg cccccagccg atggcggggg ccccggcggc ccagtcgatc
     3361 gcggcattgg cgccggtgca ggcaggtggt tcgggcgcgc gctgggccgg attgtaaggt
     3421 gtgcgggtga gatattcggc cacggcaatg cgctctgccg gggcaaggcc ggtggccatc
     3481 tcacgcatca ctccctcgtt cagcgccttc acgatggcgg cggggctgag catgccgagg
     3541 aattgcggcg agggcgcctt gggcaccgca cccatgtggc agatggcgca atgctgcgca
     3601 aaaagcgcgc ggccgggggc ggtttcgggg tctatgcggg cggcatcgcg ggcctggaaa
     3661 tcatcgaccg aggtgatgcg ggcgggcggc gggctggcag cgacggcagc caggggcagc
     3721 agcacggccg ccagccgcgc cagcgaaaac aaagggggca tgaaaggctc cttgcgcggg
     3781 cgctcaggcg agcgagcggt agaaggcgag ctgcatgtcc tcggccaggg tggcgaggat
     3841 gggcggcacc agccggtcgt ggaagtcgat atgcatatgc gcctcgaagg cggcctcgtt
     3901 gcggaaggtg gcgaccacct ggaacaggtc ggggtcggcc tcggactgga acagttcgta
     3961 gacgaggcag tcgggctcgt gctcggccac cagccgcttc agctcggcct gcaggcagat
     4021 gagatcggca cgcgcctcgg gccggcagcg cagctgggcg atgaagcttc tagcgggcat
     4081 cggcggtctc cgctgcgggg gcaaggtcgt agagcatctc gcgctcgtag gggccgtcca
     4141 tgcaggcgat catgcgggcg atcagcggct ggttggcggc agcggcgata tgcgccttgt
     4201 cggccgcggc atcgacaaag ctttcgtaga cggcgaaaag gccaggctct gccaggcgga
     4261 agaactggta atgcagcgtg cccggctcct ctgccgaaag cgcttccatc tgccgggcaa
     4321 gggtgatgaa ggcggcctcc tgttcgggcc ggatgcggaa gcgggacaga aagctgaatg
     4381 ccatgggcgc ctctccattc gccagtgtgc tcgttatggc cgaaagcagc gggcgcaggc
     4441 gcatgggcgg ccggcgcgaa gggcctggca ggcaggcgat tcgcgctagg gccatgcggg
     4501 gctggcggcc atctcccttc gggcccggtc tgtcgcgggc gtgggcagag catagccggg
     4561 gcgatagaaa aaacaatcat gaacgcatgg aaaattatgc gggcggggcc gggccgggcg
     4621 gcaggtgcgg gtgatggccg caagcgcccc gccaccttgc gcaaggggct cgcctggcgc
     4681 gcatcaaaaa acattcatgt ctggttgtaa tcgggtgaaa ctgtgttatc catcggcgcg
     4741 agaaccacag atatagtggg gacacgagga gagagggatg aacacgaaag acctgtggcg
     4801 gggcaccgcc atggccgccg tggcaatggg ggcagtggcc gcatgcagcc ccgccgtggc
     4861 agccggcgcg gatgccgccg gcacggcagc tggcaccagc gccgataccc ccgccggcct
     4921 caacgagatc gtggtgaccg ccacccgccg cgacaccaac ctccagcgca cgccgatcgc
     4981 cgtctcggcg gtggatacca acctcatccg caccgcctcc ccgcgtgatg tgggcgatct
     5041 ggccgctttc gtgcccaatt tctcggccgg cacgatcacc aatttcaacg ccgcttcctt
     5101 tgccatgcgc ggggtggggc aaacctcgat cattgtctat ttcgagccgc cggtagcggt
     5161 gctggtggat gatttcgtcg tctcctcggt gcagacccag ctgctcgaca cgttcgacat
     5221 cgcccaggtg gaagtgctgc gcgggccgca gggcacgctg tttggcaaga acaccactgg
     5281 cggcgcggtt acggtgaaga ccaagaagcc ggagctgggc attctgggcg tggatgccca
     5341 tgccgaatat ggcagcttca acaccggcaa gttccagctg gccttcaacc tgccgattgg
     5401 cgacacgatc gccgtgcggc tggtgggcgg catggaaaaa tcggatggct actacaagaa
     5461 cggcgcctgc tatggcccgg tgacggggat tgtcaccacc aacaagttcg aaggcgcctc
     5521 aggctgcctc gatggcaaga gcgcgggcgg caaggatgtg gcccaggcgc gcgccaagat
     5581 cctgtggcag ccgtcggatc gcttcaagct gctggcgcaa tatgaatggc tgcgcgatcg
     5641 ttcgcaaacc atgccatcgg tgaacgagaa tgccagctac accggcaccg gcagcttcct
     5701 caccagcacg ctgggggtga tcaacaccaa tggcgccagt tccgatccgc tgaacaatgc
     5761 cgcggtctcc tatcggcagg acgggctgat caagatgcgc gacgggcagc gcatctcggt
     5821 cgatgggttc tacctcaacg ccgattacaa gctgggcttt ggcacgctca cctcggtcac
     5881 gggctggcgc tatcagcgca gccgcctgcc caattcctat gccggcgcgg tggcgatcgc
     5941 cagcgatggc cagccgcttt ccttcttcga tgccacgcgt gacgacaacc gcagcacctt
     6001 ccagcaggaa ctgcgctttg ccaccaattt tggcgggcgc ttcgatatgg tggtgggcgg
     6061 tttctaccag catgagaaca tcgatttctg cgtggcgcag ctgctgggct tcctcgatct
     6121 gacgagcggg ccgatcgcgg gcctgggcaa ctggaacgac aacccttaca tcctgtgcaa
     6181 cgcgcaaacg tcccggtcgc gcgccgtatt cgccgagggc acctacaaga tcacgccgaa
     6241 gctgagcctg acggcgggcg gccgctacac ctgggaaaac aagacctggt ggggccgcca
     6301 gcaggtgttc atcccccagc tgaacggcgg ctgggacaca tcgatcaaca tccagaacgc
     6361 gctggatgcc aatgtgtttg cctatccggc cggcgtcatc tcgctcaccg attccgcgca
     6421 tgagcccacc tatcgcgcca gcctgggctg gcaggcccag cgcgatctct atttctatgc
     6481 cacctattcc cacggcttca aggccggcgc cttcaacgac cagatcggcg ggtttgccgc
     6541 cttcggatcg gattacaacg cctttcgcac cgcggctgcc gccaccaagc cggaaaaggc
     6601 cgacagctat gaaatcggcg tgaagagcga attcctcaac caccgggtgc gcttcaacct
     6661 caccggcttc tgggtggaat acaaggatct gcaaaagcag ctgaacgtgc cgatcgaagt
     6721 gaacggcgcg cccaaccagg tgacgatctt cgtcaacgcc gccagcgcca cggtgaaggg
     6781 catagaggcc gaagcggccg cccgcctgtt ccagggcttc accctgcgcg ctgtgctggg
     6841 ctatcaggat ggccactaca accagtatac cgcgccatcg gccggctata atctggcctc
     6901 ggcgccgctc gatcgcacgc cgcgctggca gtggacgctg gatggcacct atgagcggcc
     6961 gttgaaccgc cagttcaaat gggccatcga tgcctctgcc gcctacaccg gcagcaacct
     7021 caacacccag tcgatcaccg atccggcggg caacactttc ctcaacgccc gcacgctggt
     7081 gaatgcctcg ctcaccctct cgcagatcca tgatgattat tacatcaagg tggtgggccg
     7141 caatctgacc gacgagcgct atcgcaccgc ctcgcagaat gtcgccggcc tgtggctcaa
     7201 ttcgaactat ggcccgccgc gttatgtggg gctggagctg ggcgcgaaat tccgcggctt
     7261 ttgaaaaggg cgccggcccg ccgtggccgg ccgccccctt ttttccggga gacagtccat
     7321 gcggctcgat ttcgatgagt ggaacattca tgtggggccc gatcacccct tcaaccggcg
     7381 cgcggcccct atcgacaatg gcaccgcgcg ccccgcgccc gatcgctata ccagcccggc
     7441 cttcgcccgc gccgaatggg agcgggtgtt tgccagaagc tggctgctgg ccggccctgc
     7501 tgccgatatc cccgagccgg gcgatttcat gcgcttcgac attggcggcg aaagcttcct
     7561 catcgtgcgc ggggaggagg ggcagatcca cgcccattac aacgtctgcc cgcatcgcgg
     7621 cagccggctg gtgaccgagg atttcggttc tgtcgcgcag ttttcctgcc cgttccattc
     7681 gtggaaattc gcgctggatg gccgctgcgc cgcggtgacc gatgccgcca ccttccgccc
     7741 cgaagtgctg gcccatggca cgcagctctc cagcgtgcgc tgcgaggtgg cggtggggct
     7801 ggtgttcatc gccatggatc cggcgatccg cccgctggcc gaatggctgg cccccatcct
     7861 gccgcagctg gcgctgtatg acatcgaccg gatgtatgtc atccagcacc gccagtcggc
     7921 atggggggcg aactggaagg gcggggtgga tgccttcgcc gagatctacc acctccacgc
     7981 cgtgcacccg caaacccagg gcctgatgga cgaccgcacc cagatcgacc tgtggccggg
     8041 cggcctgtcg cgccagttcg tgccctttgc ccagccctcg ccgcgctatg ccgatcagga
     8101 aggggtgaac ccgggcatcc agctgatgct gcgcgatgcc ggcatcgacc cggccgccta
     8161 caccggcacc gcccgcgaaa cgcgcgccgc cgtgcaggcc gccaaacgcc gccgctcggc
     8221 cgaactgggg ctgggctacg agaaattctc cgacagccaa ttgtcggatt cggtggtcta
     8281 ttcgctgttc cccaatgtgc agatcggctg ccacccggaa gcagtgttcc tccaccgctt
     8341 cctgccgcac gccagcgatc ctgcgcgctt cacctacgac acctgcatcc tctatcgcca
     8401 tatcgatgcg cccggctatt ccgcccccgc ctggatgggg ctgggcgacg aggtggactt
     8461 ctccggccgc acccggcccg cgatcacccg caccggccct ggcgaaccac ccggccttgg
     8521 cgaagtgctg gaccaggaca gcgacctgtt gccggtggtg cagcagggcg ccggctcgcg
     8581 cggctttcgc gggcccctgt ggagcgaaca ggaaggccgc ctgcgccact tccacgccga
     8641 gctggaccgg atgatggcgg cggggtgagg gggcaggaga ggaagggagg gggcgagggt
     8701 tgacaccctc gcgctcccat tacctgtaga ggttgcatgg tctttcggcg tggcgcccgg
     8761 cggcgcagcc gcaatcatca aagcctccgg cggtgcgatg gcgcgccggg gcgcaatggt
     8821 gcgcgacgct gacaggtaac gggagcgcga gggtgttaac cctcgcatcc cttcctttcc
     8881 ccttcaaaac ggcctgatcg tcaccccatt gtcgatcacc agttcggcgc cggtgatgaa
     8941 gcggctttca tcggcagcaa ggaacaccac cagcgcggca acatcgtccg ggtggcccgg
     9001 ggtgccgggg ggcagcacgc cctgggggat ggttctggcc tgccccacgc ggccctcggc
     9061 ggcttgcacc atgggtgttt cgatggcgcc ggggtgcacc gaattgcagc ggatcttgta
     9121 gcctttttcc tggcacatca cggcgatgga tttgctcatc gcgcgcacgg cgcccttggc
     9181 ggcggaatag gcgaagaaga tcggatagcc caggatcgcg cccaccgagc tcatgttgat
     9241 gatcgagccg ccgccgcttt tgtgcatcag cggcagggcg tgtttgcagc ccaggaacac
     9301 gccatcgatc atgatcgcgt tcaccttgtg gaactgttcg aggctggtgg cctcgatatc
     9361 ggcgttgagc accacgccgg cattgttcac cagcacgtcc agccggccaa agcgctgttc
     9421 cacagcggcc atggtggcga tccactgcgc ttcgctggcc acatccagcg gcagggcgat
     9481 ggccgtgccg ccaatctcgc gggccaccgc ttctgccgcc cccagcgcca catcggtgag
     9541 gatgacctgt gcgccttcgc gtgccatggc gcgcgcatct gccgcgccca gccccgatcc
     9601 tgcgcccgtg acgagcgcca ccttgcctgc cagacggccc attgctgctc tcccttgtgg
     9661 ctggcgctgg cgctaggccg cgccgccttc gttgaaataa tcgggcatgc cggccggcca
     9721 caccacgccc cattgcgcct gcccgccatc cagcaggatc agatcgccat tgacgaaacg
     9781 cgcggcgggc gaggcgagat aggcgatcgc ctcggccaca tcccaggcat cgccgcgggt
     9841 tttcatgggg ttggccatgt ggaagcgggc cagcgcctct tccggataca tgcggaagcc
     9901 ctcggtctcg atcacgccgg ggccgatgca gttgatgcgg atattgtgcg gcgcccattc
     9961 ggtggccagc gttttggaca ggtagatcac gccggcccgg gcggcgcagg tgtgggcggc
    10021 ctgcggcatc ccccgttcca cattggcaac gatgttgatg atattgcccg ccgccccatc
    10081 ggcgcgccag cgccgggcgg cttcctgcat catccaccat gtgccgttga ggttggtgtc
    10141 gatcaccgcc agccagccct tgcgggaaaa atcgatggca tcctgcggga actggccgcc
    10201 ggcattgttg accagcgtgt gcagcgcgcc aaagcgatca tgcacgcggc cgatcagcgc
    10261 ttccaccgca tcggcatcgc ggatgttggt ggccaggggg aagatggcgc ggccggtttc
    10321 gccctcgatg aagcgggcgg tggcagccag cttctcctcg tcgcgcccgc agatcgccac
    10381 ttcggcgccc agccgcgcca gcagcagcgc agccgccttg ccaatgccgc tgcccccgcc
    10441 cgacaccagc gccacctggc cggccagcag atctgtgcgg aacacgcttt ggcgggcggc
    10501 cagcgcggca tcgtccagcc cgcgggcggg gcgggtggtg tcttcagggt cggggcggaa
    10561 gctgcgtggg ggt
//
LOCUS       DASRYE010000279         4267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_31309,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000279 DASRYE010000000
VERSION     DASRYE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31309"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFF98_14355"
     CDS             <1..647
                     /locus_tag="VFF98_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64863.1"
                     /translation="PHHPASPEGVALNADVPVIISTMLEDAGLFFGHFDLTEEALAAL
                     LAEKYGEAGQRLLPLYRAHTPAKSPYLLHARIITDAGFRRYAHAQAAARHAGGAAPVW
                     MYRWDWPSPAWDGLFGAAHAMDVSASFARVRDSLLGGGVANGRRLAGALSSALAGFAR
                     DGQPGGAWRGPWQPYDPHTRACLLLGEDDVLAHDPDADLRAFWEALPMAASVAG"
     gene            complement(654..1337)
                     /locus_tag="VFF98_14360"
     CDS             complement(654..1337)
                     /locus_tag="VFF98_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU64864.1"
                     /translation="MSPKVLIIEDDAAIRRLLQLCLARGEYAAVAAENARAGLAALAI
                     DKPEAVLLDLGLPDRDGLELVPLVKAAGCALLVISARDETAQKVAALDLGADDYITKP
                     FDGDEVLARLRTALRHRLAGHMAGGPALVKLGQVEVDLAARRVLRAGAEVHLSPKEYG
                     VLAELARHPGRVITHAQLLRAVWGPGHEQDVEYLRVAARGIRRKLEDEPAAPTLLRNE
                     PGVGYRLMG"
     gene            complement(1334..4021)
                     /locus_tag="VFF98_14365"
     CDS             complement(1334..4021)
                     /locus_tag="VFF98_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZU64865.1"
                     /translation="MTEDDRPDPDALLRTLNQAGGSGGEHGARGRLKVFLGAAPGVGK
                     TCKMLEDGAARAAAGVDVVIGVVETHGRKETVERTAGFEILPRRTIRHRGQALEEMDI
                     DAILARHPALVLVDELAHTNASESRHDKRWQDVEEILDAGIDVYTTLNIQHLESLNDV
                     VASFTRVRVRETLPDRVLEQAEIEVVDLPPGELIERLREGKVYVPEEAGRALAHFFSR
                     SNLSALRELALRRAAQAIDAQMLDLVRAQALAGNWAAGERLVVAVSEQPGAAELVRTA
                     KRMADALRAPWTAVHIETPRDATFTDAEATRLAAHLQLAAQLGGQTAVIPAESVTAGL
                     FVFLREARATQLILGKASRSRWHEWRHGSVVDRMVRATPGVAVHVLGLEAPAEPAGWR
                     LPQPGGARQPWAAYGLSCALVGLVTLAGVSIASLGSITNIALLYLLPVLVAATRFGLR
                     AGLVAGLAGSLAYNFFFIPPVHTFTIADPQNLITVLVLLGVAVLVSQLASRMREQALL
                     AQRSAAQNSALAGFARLLTGVRRAEELGQILCAEVARLLDVRTVLLLPEAGELVLSAA
                     FPPEDRLETLELAASRWAFDNGRPAGRGSDTLTASEWLFHPIAAGGRALAVFGVARED
                     GQSPLRADQRPLLASLLDQAGLSLERIALEGEMAKVETLREQDRLRSALLSSVSHDLR
                     TPLTAILGTLAALSAADEAQSRQLATARAEAERLSRFVANLLDMARIEAGAMARASEP
                     VDIAEAVASAVHDLRPQLADHPIRLDVSPELPFVLVDPQLFHHCLINLFDNAAKYSPA
                     GAPITVAARRDREGLSLMVLDEGPGLPPGTEKRIFETFTRIEGSDRRGGTGLGLAIVK
                     GFAEAMGLGVSAANRAEGQGACFTLRFDAAHLRLGASGE"
     gene            complement(4018..>4267)
                     /locus_tag="VFF98_14370"
     CDS             complement(4018..>4267)
                     /locus_tag="VFF98_14370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HZU64866.1"
                     /translation="LPADLVTASASGLDPHISPASAAAQAARVAKARGVPLDRIQLLV
                     ARFTEDRDFGFLGEPRVNVLELNLALDTMARGEAHTTP"
BASE COUNT          626 a         1569 c         1453 g          619 t
ORIGIN      
        1 tgccccatca cccggcctcg cccgaggggg tggcgctcaa tgccgatgtg ccggtgatca
       61 tctccaccat gctggaggat gccggcctgt tcttcggcca tttcgacctg accgaagaag
      121 ccctggccgc cttgctggcc gaaaaatatg gcgaggcagg ccagcgcctg ctgccgctct
      181 accgcgcgca tacgccggcc aaatcgccct atctgctgca tgcgcggatt atcaccgatg
      241 cgggctttcg ccgctatgcc catgcccagg ccgcagcgcg ccatgcgggg ggcgcggcgc
      301 ctgtgtggat gtatcgctgg gattggccaa gcccggcctg ggatggcctg tttggcgcgg
      361 cccatgccat ggatgtgtcg gccagctttg cccgcgtgag agacagcctg ctgggcggcg
      421 gcgtggccaa tgggcggcgg ctggcgggcg cgctgtcgtc ggcgctggcc ggctttgccc
      481 gcgatggcca gcccggcggc gcgtggcgcg ggccctggca gccctatgat ccgcacaccc
      541 gcgcctgcct gctgctgggc gaggacgacg tgctggcgca tgacccggat gccgatctgc
      601 gcgccttctg ggaggcgctg ccgatggcgg ccagcgtggc gggctagagg cgttcagccc
      661 atcagccgat accccacgcc cggctcgttg cgcagcaggg tgggcgctgc gggctcgtct
      721 tccagcttgc ggcggatgcc gcgggcggcc acgcgcagat attccacatc ctgctcatgc
      781 cccggccccc acaccgcgcg cagcagctgc gcatgggtga tcacccggcc aggatggcgc
      841 gccagctctg ccagcacgcc atattccttg gggctgagat gcacttccgc gcccgcgcgc
      901 agcacccggc gggcggccag atccacctcc acctggccca gcttcaccag cgccggcccg
      961 cccgccatat ggcccgccag ccggtggcgc agcgcggtgc gcaggcgcgc cagcacctcg
     1021 tcgccatcga agggcttggt gatgtagtca tcagccccca gatcgagggc ggccaccttc
     1081 tgcgccgtct cgtcccgcgc ggaaatcacc agcagcgcgc agcccgccgc cttcaccagc
     1141 ggcaccagct ccagcccgtc gcggtcgggc aggccaaggt ccagcagcac cgcctcgggc
     1201 ttgtcgatgg cgagcgccgc cagccccgcg cgcgcgttct cggccgcaac ggcggcatat
     1261 tcaccccgcg ccaggcacag ctgcaacagc cggcggatcg ccgcatcatc ctcgataatc
     1321 agcactttcg ggctcattcg ccgcttgctc ccagccgcag gtgggccgca tcgaagcgca
     1381 gggtgaaaca ggccccctgc ccttcggccc ggttggccgc tgatacaccc agccccatcg
     1441 cctcggcaaa gcccttgacg atggcaaggc caagcccggt gccgccgcgc cggtccgatc
     1501 cctcgatgcg ggtgaaggtt tcgaagatgc gcttttccgt gcccggtggc aggcccggcc
     1561 cctcgtccag caccatcagc gaaaggccct cgcggtcgcg ccgggcggcc acggtgatgg
     1621 gggcgccggc ggggctgtat ttggcggcat tgtcgaacag gttgataagg cagtggtgga
     1681 acagctgcgg atcgaccagc acgaagggca gttccggcga gacatcgagg cggatcgggt
     1741 gatcggccag ctgcgggcgc agatcgtgga cggcgctggc caccgcctcg gcaatatcca
     1801 ccggctcgct ggcgcgggcc atggcgccgg cctcgatgcg ggccatgtcg agcagattgg
     1861 caacgaagcg gctgagccgc tcggcctcgg cgcgggcggt ggccagctgg cggctctggg
     1921 cctcatcggc cgcagaaagc gcggccagcg tgccgagaat ggcggtgagc ggcgtgcgca
     1981 gatcatggct gacgctggac agcagcgcgc tgcgcagccg gtcctgctcg cgcagggttt
     2041 ccaccttggc catctctccc tccagcgcga tgcgttccag cgacagcccc gcctgatcga
     2101 gcaggctggc cagcaggggg cgctggtcgg cacgcagggg gctctggcca tcctcgcgcg
     2161 ccacgccaaa caccgccagc gcccgcccgc ccgcggcaat cgggtggaac agccattcgc
     2221 tcgccgtcag cgtatccgat ccgcggccgg ccgggcggcc attgtcgaag gcccagcgcg
     2281 aggcggccag ctccagcgtc tccaaccggt cttcgggcgg gaaggcggcg ctcagcacca
     2341 gctcgcccgc ttcgggcagc agcagcaccg tgcgcacatc gagcaaccgc gccacttcgg
     2401 cacacaggat ctggcccagc tcctcggccc ggcgcacccc tgtcagcagc cgggcaaagc
     2461 cggccagcgc ggaattctgc gcggcgctgc gctgggccag cagggcctgc tcgcgcatgc
     2521 ggctggccag ctggctgacc agcaccgcca cccccagcag caccagcacg gtgatgaggt
     2581 tctgcggatc ggcaatggtg aaagtgtgca ccggcgggat gaagaagaaa ttataggcaa
     2641 ggctgcccgc cagccccgcc accagcccgg cccgaaggcc aaagcgcgtg gccgccacca
     2701 gcaccggcag cagatagagc agcgcgatat tggtgatgct gcccaggctg gcgatcgaca
     2761 cccccgccag cgtgaccagc cccaccagcg cgcaggacag gccataggcc gcccagggct
     2821 ggcgggcgcc gccgggctgc ggcaggcgcc agccggcagg ctctgccggc gcttccagcc
     2881 ccagcacatg cacggccacg cccggcgttg cccgcaccat cctgtccacc accgagccgt
     2941 ggcgccattc gtgccagcgg ctgcggctgg ccttgccaag gatcagctgg gtggcgcgcg
     3001 cctcgcgcag gaagacgaac aggcccgccg tcacgctttc ggccgggatc accgctgtct
     3061 gcccgcccag ttgcgcggcc agctgcagat gggcggcgag gcgcgtggcc tcggcatcgg
     3121 tgaaggtggc atcgcgcggg gtttcgatat gcaccgccgt ccagggcgcg cgcagggcat
     3181 cggccatgcg cttggccgtg cgcaccagct cggccgcgcc cggctgctcg ctcaccgcca
     3241 ccaccagccg ctcgccggcc gcccaattgc cggccagcgc ctgcgcgcgc accagatcca
     3301 gcatctgcgc atcgatcgcc tgggccgccc ggcgcagcgc cagctcgcgc agcgccgaca
     3361 ggttcgagcg cgagaagaaa tgcgccagcg cccgccccgc ctcttccggc acatagacct
     3421 tgccctcgcg cagccgctcg atcagctcgc ccggcggcag gtccaccacc tcgatctcgg
     3481 cctgttccag cacgcggtcg ggcaatgtct cgcgcacccg cacgcgggtg aaggaggcga
     3541 ccacatcgtt caggctttca aggtgctgga tgttgagcgt ggtgtagaca tcgatgcccg
     3601 catcgaggat ctcctccaca tcctgccagc gcttgtcgtg gcggctttcg gacgcattgg
     3661 tgtgggccag ctcgtccacc agcaccagcg ccgggtggcg ggcgaggatg gcgtcgatgt
     3721 ccatctcctc cagcgcctgg ccgcgatggc ggatggtgcg gcgcggcagg atctcgaaac
     3781 ccgccgtgcg ctccaccgtt tccttgcggc cgtgggtttc caccacgccg atcaccacat
     3841 ccacgccggc tgccgcccgc gccgcgccat cttccagcat cttgcaggtt ttgccaacgc
     3901 ccggcgccgc cccaaggaac accttgaggc ggccacgcgc gccatgctcc ccgccactgc
     3961 cgcccgcctg gttcagcgtg cgcagcaggg catcgggatc tggtctgtcg tcttcggtca
     4021 cggggtggtg tgtgcctcac cgcgggccat cgtgtcgagc gcgagattga gttccagcac
     4081 attcacccgc ggctcgccga ggaaaccgaa gtcgcgatct tcggtgaatc gcgcgacgag
     4141 aagctggata cgatccaacg gaacgccgcg tgccttggcg acgcgcgcgg cctgcgcggc
     4201 ggccgaggcc gggctgatgt gcggatcgag gccgctggcg ctggctgtaa ccaggtcggc
     4261 cggaagc
//
LOCUS       DASRYE010000280        14245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_211625,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000280 DASRYE010000000
VERSION     DASRYE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14245
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211625"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VFF98_14375"
     CDS             complement(<1..924)
                     /locus_tag="VFF98_14375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU64867.1"
                     /translation="MTATASARPPIIDLHSHWFSPAAVEILGQQPGAPRIATATDGAR
                     AIHRAGPGSNGLPFALGEHWFDIDLRLKHLDASGVAHQLISWPTTLGVDSLANGTDWR
                     PLWAAYNDDLSALVRAHPTRFSAVAALSTHDIAWSVKELERAHEKLGLIGAVLPVNGF
                     FTSASARAFAPLFEAAQRLGSHIYLHTGYASPAIPGQPPIVEHLDNAAARWVLDNAWH
                     FASSVATLAFSDFLDPYPDVTVQVAMLGGSGLAALVAEQVEISAARSGISDVRARFRN
                     LWLDTGAAGNGPAAVAAAIRVLGADRVVFGSD"
     gene            complement(933..2213)
                     /locus_tag="VFF98_14380"
     CDS             complement(933..2213)
                     /locus_tag="VFF98_14380"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64868.1"
                     /translation="MTIATQAAPAIARPDHAARPDTSPRTAEPYPADWLALARTLADE
                     LRPGAIARDALGTLPVEEVRRLRESGLVNLLYPADLGGGGGTLREAAWSVLEIAKADG
                     SVGAVLGFHFYNSLVPLLLDYNGANDAVVREAARQRWQWGNVTQYVNVDFVAEHHPNG
                     GYTISGTKKWNTGTPIAEHTTLLFVHPDRTHYLYAHIPTDRAGLRFHEDWDQIGLRGA
                     DSSTISFDQVRIHAEEVLHWGHDGQQTGPLPLWATFGAVFYSAVYLGNTLAALEAARD
                     YATAGRRQSVLPGAATTAEDVLVQTQFADIWLKAKAAEALFETTIARLAAGWERRHEL
                     SEEDRGLLAIETLALRTYTSQVALEVTAQIFEFGGGKATGRSYGFDRFWRNVRTLANH
                     DPLVLAQKTLGAFALSGQPPRFPAKFPAKNPAAK"
     gene            complement(2219..3508)
                     /locus_tag="VFF98_14385"
     CDS             complement(2219..3508)
                     /locus_tag="VFF98_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64869.1"
                     /translation="MPVPSLTQAPWQAGSAESHAAARPASPHVSLAAIMLLGIVCVVD
                     AMIISSLLTPIKADLGLNDEAFGRIAAAFTLAGVLGAPLFGYAAGRLGRKPVLIAGAV
                     LWSIASAFNALAAGVAGLLFWRAVTGFGEAAYQGLAPGWLADLYDKRWRNLVFSVFMV
                     RNKIGSALALALGAWLAGAYNWHIAFLVSGLPGLVCAAWLALLPEPRPGAADGYAEAP
                     RHLTWREQLSVLRHGRYAAHLAALALFFSGTATAQMWAPAYLHRVYALSNAAASGFLA
                     TVLLATLPVGLVGGYVFGRWLSPYRWGLTAALAATSLLAAGFFAQAFLTRDLATAKLF
                     IILAICAFGSTAGSLTTLLVELVPPHLRTAAGALGAVVVAAISGALAPWLLGYLSDRI
                     GLDRAILVGPAAYALAGLIWIASLVALRFSPLSPTKD"
     gene            3696..4628
                     /locus_tag="VFF98_14390"
     CDS             3696..4628
                     /locus_tag="VFF98_14390"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HZU64870.1"
                     /translation="MKVGIVGAGMVGSMAAYAMALQGTAAEIVLVDRNADLAAAQAED
                     ISHATPFLQPRRIVAGDYAALAGAGVVILAAGVPQKPGESRLSLLSRNADVFAEVVGQ
                     VRAAAPEALLVVASNPVDIMTMVAQRASGLPPERVIGSGTLLDTARFRQLLASRLGVA
                     AQSIHASVLGEHGDSEVLAWSSAQVGGKPLEPGAALLGAPITAEVRAAIDEGVRRAAY
                     RIIAGKGATWFGIGAGLARIVQAIRDDQRAVLTVSIRMPEVLGVADVALSLPRVVGRE
                     GVLATLWPDLAPDEEAALRASAEVLQAAAGGLWR"
     gene            complement(4640..6139)
                     /locus_tag="VFF98_14395"
     CDS             complement(4640..6139)
                     /locus_tag="VFF98_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZU64871.1"
                     /translation="MAAERPAIAGAGRTTHAALLDELDALIAAVRSETRQRMELWRAG
                     GIRPAFLPSAENLAAYLALRHRDLRPLQRRLMVLGLSSLGRLESRVMPTLLAVRAALA
                     GMAGQPAEAVGGEDFFAGEQRLEARARQLFGARRQQRQAALMVTCPSEAADDPAFMRD
                     LAARGVEAIRINCAHDGPEAWARMIAHLRAGEGGQAAHVFMDLAGPKLRTGGLRHHHD
                     VKRLFPGDRLALVAPGGLDRKVPADVACTVECTLPEAIAAAAPGERLLIDDGKIACAI
                     EMVGEGMLIASVTRCKEEGAKLKPEKGINLPDTDLQVPALTAKDRADLAFVAAHADGV
                     EFSFVQEVADVELLQAELARLRPDDWQELALILKIETARAVHNLPDILLAAAARQPVA
                     IMIARGDLAIEIGFARLAEMQEEILWIAEAAQVPVIWATQVLEHLVKEGLPNRGELTD
                     AAMAARAECVMLNKGAFLFEAIAALDGLLARMDDHVHKKTPQLRRLTSW"
     gene            6333..6677
                     /locus_tag="VFF98_14400"
     CDS             6333..6677
                     /locus_tag="VFF98_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64872.1"
                     /translation="MAQVISQRLRDGRPLWHEQLAFRLAGLGGLWGFALFARWLFWLQ
                     QRAPAVAGRPCMLAGCLVAVAVLQAGLALALAGPGLLRQVPWPGIRRIEPGTRRIGPG
                     GPSPLHRPGPHA"
     gene            6874..8085
                     /locus_tag="VFF98_14405"
     CDS             6874..8085
                     /locus_tag="VFF98_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU64873.1"
                     /translation="MDGERRVPWLGMMVALGISGWLMLSGVNTMSIALLGPVWLLSLW
                     LDWLRPVARPAIRIAINRPDIDALTREAMTRTLEGVSLPLVLMERHRIMAANGAARAA
                     FGAHIVGQDARIALRHPEAMRLLDMPSGGSVTIRGLTGTGSLWQLTRQTIDSRFWTIE
                     LRDRTTEADLGRAHTDFVANASHELRTPLSSIIGYVETIADDPGRMEPDTTRRFLGTV
                     LREARRMQNLVSDLMSLSQLEAEKHDLPSERVDLGRLAHRVVGEFAPGPQGSGPRLRL
                     SRPDETISVAGDVKQLEQLLRNLIDNALKYADGSQPVEVSLAGAPRGMVELVVADHGQ
                     GIAPEHLPHLTRRFYRTDPGRSRAAGGTGLGLAIVKHIVERHRGRLDITSQLGVGTQV
                     HVRLPLAAAPS"
     gene            8346..9029
                     /gene="phoU"
                     /locus_tag="VFF98_14410"
     CDS             8346..9029
                     /gene="phoU"
                     /locus_tag="VFF98_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZU64874.1"
                     /translation="MVAEHTVKAFDEEITSLRGLIAEMGGLAEVAISESMDALTHGSL
                     QLAEEVIARDKRIDALETQVEQLAVRIIALRAPMADDLRDIIAALKIAGILERIGDYA
                     KNIAKRVSRIERREVFEPLTLIPAMAEVAAEMVHDVLTAFAARDPQLAIDVVERDAKV
                     DAFYDSIFRNMVSFMMENPSTITAAAHLLFVARNLERIGDHATNVAEQVYYAATGEYP
                     AERTERPEN"
     gene            9064..9753
                     /gene="phoB"
                     /locus_tag="VFF98_14415"
     CDS             9064..9753
                     /gene="phoB"
                     /locus_tag="VFF98_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZU64875.1"
                     /translation="MNPPRLLLVEDDPALAELLEFRFRAEGYTVSATADGDEALLRAA
                     EEAPDLVVLDWMIEGTSGIEVCRRLRRDKATAHVPIIMLTARGAEDDMIRGLETGADD
                     YITKPFSPRELIARVAAIMRRIRPALAGESLTVGDLTLDATAHRVTRRGQMVQLGPTE
                     FRLLKFFMEHPGRVFSRGQLLDAVWGTDSDIELRTVDVHIRRLRKGIEVDGAPDPVRT
                     VRSAGYALEAV"
     gene            complement(9821..11389)
                     /locus_tag="VFF98_14420"
     CDS             complement(9821..11389)
                     /locus_tag="VFF98_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZU64876.1"
                     /translation="MFGRVKPLDAILATAERKSLHRSLGAVQLTLLGIGCVIGTGIFV
                     LTAAGAQKAGPGLMLAFAIAGVICVVAALCYAEIAAMLPVAGSAYTYTYAVMGELLAW
                     TVGWALILEYAIAASAVAVGWSGYFTGTILGKTMGVHLPTALSAGPLVLGGAPGGFIN
                     LPATVISLLITGLLMLGTKESARFNAFLVAIKIAALTVFVVLTAPHMHGDNFSPFLPA
                     GVFGGFGTGVGAVGAAATIFFAYVGFDAVSTAAEETRDPQRNVPIGLVGSLLICTLFY
                     MAVAAGAVGSIGGQPIMGGDGTPLPAGSPELASQCILPQYADRLVCSHEALAHVLHVI
                     GFGAVGNFMGIAAFIALPSVVLILLFGQTRIFFVMARDGLLPEALSRVHPKWKTPYIV
                     TALTGLATALGAAFFPVGELSDIANAGTLYAFMMVAIAVLILRRTEPNRHRPFRTPLV
                     WLIAPAAIFGCVFLFFNLPLAAMLVLPAWAAIGLVVYFGYSRSHSHVGRGLVEVHEVD
                     EDAPGLPVPPAPEF"
     gene            11585..12457
                     /gene="panB"
                     /locus_tag="VFF98_14425"
     CDS             11585..12457
                     /gene="panB"
                     /locus_tag="VFF98_14425"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZU64877.1"
                     /translation="MSTTFQIDTATSRANPTPAPMKRLTIPALRARKAGGHTAEPIVM
                     LTAYTARQAQLLDPHCDLLLVGDSLGQVIYGLPSSLPVTLEMMAAHGAAVVRGSWHAV
                     VVVDMPFGSYEQSPAQAFENAARLLKETGAAGVKLEGGAAMAETVAFLSARGIPVMGH
                     VGLTPQAVNQLGGYGARGRSAAEAEKILADARALDEAGAFAVVVEGVVEPIAIAVQQA
                     LACPVIGIGGSAQCDGQVLVAEDMLGLFERVPRFVKRYEDMAGRISEAVASYAADVRA
                     RSFPGAEQTYQPKR"
     gene            12467..13522
                     /locus_tag="VFF98_14430"
     CDS             12467..13522
                     /locus_tag="VFF98_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HZU64878.1"
                     /translation="MSLAAALRRHLLGPALFTLLCLALAGAYSWQASPSVPLLLGQLW
                     VVVVLAVLEVSLSFDNAVVNAALLADMDAVWRKRFLTWGMVFAVFGTRILFPLAIVGL
                     SAGLDPVAAVRLSLTEPARYEEIVTAAHLPIAGFGGAFLALVGLRFFLDQGKQRHWLR
                     WLERPLHGLGRVEAAAIALVLAALLAGGAWLAPQDAHGWLTGGIVGIITFVLVEALGN
                     LLGAGDGGHGATVAVARSGLGAFLYLNVLDASFSLDGVIGAFALSNNMVIIALGLSIG
                     AAFVRALTLILVERDVLGQLVFLEHGAFWAILALAGIMLSAPLVEVPESITGLVGAGL
                     IAVAFWQSLRLRQRGGA"
     gene            complement(13799..>14245)
                     /locus_tag="VFF98_14435"
     CDS             complement(13799..>14245)
                     /locus_tag="VFF98_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64879.1"
                     /translation="LWYRLAEIVVRIPALAQRAGDPPLLARAFLSRFARELNPQVKGF
                     APDALAAIDSWSWPGNVRELENRVKRAAIMAEGKLVTAADLDLAAPQEPDAQLLNLKA
                     AREQADRRVIRHALARSEGNISNTARLLGISRPTLYDLLKQYDMQG"
BASE COUNT         2347 a         4798 c         4895 g         2205 t
ORIGIN      
        1 atcggagccg aacaccaccc tgtctgcccc cagcacgcgg atggccgcgg ccaccgccgc
       61 cgggccattg ccggcagcgc cggtatccag ccagaggttg cggaaacggg cgcgcacatc
      121 gctgatgccg ctgcgcgcgg ccgagatctc cacctgttcg gccaccagcg cggccaggcc
      181 cgatccgccc agcatcgcca cctgcaccgt cacatccgga tagggatcga ggaaatcgct
      241 gaaggccagc gtggccaccg aggaagcgaa atgccaggca ttgtccagca cccagcgggc
      301 cgcggcattg tcgagatgtt caacgatggg gggctggccg gggatggccg ggctggcata
      361 gccggtgtgc agatagatgt ggctgcccag ccgctgcgcc gcctcgaaca ggggggcaaa
      421 ggcgcgggcc gaggccgagg tgaagaagcc gttcaccggc agcacggcgc cgatcagccc
      481 cagcttctca tgggcgcgct ccagctcctt caccgaccag gcgatgtcgt gggtggagag
      541 cgccgccacc gcgctgaagc gggtgggatg ggcgcgcacc agcgccgaga gatcatcgtt
      601 ataggccgcc cacagcgggc gccagtcggt gccattggcc agcgaatcca cgcccagcgt
      661 ggtcggccag ctgatcagct gatgggccac gcccgaggca tcgaggtgct tcaggcgcag
      721 gtcgatgtcg aaccagtgtt caccaagggc aaagggcagg ccgttcgatc ccgggccggc
      781 gcggtggatg gcgcgggcgc catcggtcgc cgtggcgatg cggggggcgc cgggctgctg
      841 gcccaggatc tccaccgctg cgggcgagaa ccagtggctg tgcagatcga tgatgggcgg
      901 gcgggcagat gcggttgcgg tcatggcggg cctcacttgg ctgcggggtt cttggcgggg
      961 aatttggcgg ggaagcgcgg cggctggccc gaaagggcaa aggcgcccag cgtcttttgc
     1021 gccagcacca gcggatcgtg gttggccagc gtgcgcacgt tgcgccagaa gcgatcgaag
     1081 ccatagctgc ggccggtggc cttgccgccg ccgaattcga agatctgcgc ggtcacttcc
     1141 agcgccacct gcgaggtgta ggtgcgcagg gccagcgtct cgatggcgag caggccgcga
     1201 tcctcctcgc tcagctcgtg gcggcgttcc cagccggccg ccagccgggc aatggtggtc
     1261 tcgaacagcg cctcggccgc cttggccttc agccagatgt cggcaaactg cgtctgcacc
     1321 agcacatcct cggcggtggt ggcagcgccc ggcagcaccg actggcgccg gccggcggtg
     1381 gcataatcgc gcgctgcctc cagcgctgcc agcgtgttgc cgagatagac cgctgaatag
     1441 aacaccgcgc caaaggtggc ccacagcggc agcgggccgg tctgctggcc gtcatggccc
     1501 caatgcagca cttcctcggc atggatgcgc acctgatcga agctgatggt gctgctgtcg
     1561 gccccgcgca ggccgatctg gtcccaatcc tcgtggaagc gcaggccagc gcgatcggtg
     1621 gggatatggg catagaggta atgggtgcgg tcggggtgga cgaagagcag ggtggtgtgt
     1681 tcggcaatcg gcgtgccggt gttccacttc ttggtgccgc tgatcgtgta gccgccattg
     1741 ggatgatgct cggccacaaa atcgacattc acatattgcg tgacattgcc ccactgccag
     1801 cgctggcggg cagcctcgcg caccaccgca tcattggcgc cgttgtaatc gagcagcagc
     1861 ggcaccagcg aattgtagaa atggaagccc agcacggcgc ccaccgaacc atcggccttg
     1921 gcgatttcca gcacgctcca ggccgcctcg cgcagggtgc cgccgccgcc gcccagatcc
     1981 gcggggtaaa gcaggttgac aaggccgctc tcgcgcaggc ggcgcacttc ttccaccggc
     2041 agcgtgccca gcgcatcgcg ggcaatggcg ccggggcgca attcgtcggc cagcgtgcgg
     2101 gcaagggcca gccagtcggc cggatagggc tcggcggtgc gggggctggt atcggggcgc
     2161 gcggcatggt cggggcgcgc aatggcaggc gccgcttggg tagcgatggt catggcggtc
     2221 agtccttcgt gggagaaagg ggagaaaaac gcagggcaac aaggctggcg atccagatca
     2281 ggccagccag cgcataggcc gcagggccaa ccagaatggc ccgatccagc ccgatgcgat
     2341 cggaaagata gcccagcagc cagggcgcca gcgcacccga gatggccgcc accaccaccg
     2401 cgcccagcgc gccggcggcc gtgcgcagat gcggcggcac cagctccacc agcagcgtgg
     2461 tgaggctgcc ggcggtggag ccgaaagcgc agatggccag aatgatgaag agcttggcag
     2521 tggccagatc gcgggtgaga aaggcctggg cgaagaagcc cgccgccagc agcgaggtgg
     2581 ccgccaacgc cgccgtcaac ccccagcgat agggcgacag ccagcgcccg aagacatagc
     2641 cgcccaccag ccccaccggc aaagtggcca gcagcaccgt ggcgagaaag cccgaggcag
     2701 ccgcattgct cagcgcatag acgcggtgca gataggccgg cgcccacatc tgcgccgtgg
     2761 ccgtgcccga gaagaacagc gccagcgccg ccagatgcgc cgcatagcgg ccatggcgca
     2821 gcaccgagag ctgctcgcgc caggtgaggt ggcggggagc ctcggcatag ccatcggcag
     2881 cgccggggcg cggttcgggc agcagcgcca gccaggccgc gcacaccagc cccggcagcc
     2941 ccgagacgag gaaggcgata tgccagttat aggcccctgc cagccacgcc cccagcgcca
     3001 gcgccagcgc cgagccgatc ttgttgcgca ccatgaacac cgagaacacc agattgcgcc
     3061 agcgcttgtc gtagagatcc gccagccagc ccggagccag gccctgatag gccgcctcgc
     3121 caaagccggt gaccgcgcgc cagaacaaca ggcccgccac tcccgccgcc agcgcgttga
     3181 aggccgaggc aatgctccac agcaccgccc ctgcaatcag caccggcttg cgccccagcc
     3241 gccccgccgc atagccgaac aacggcgccc ccagcacgcc cgccagcgtg aaagcggcgg
     3301 cgatgcggcc aaaggcctcg tcgttcagcc cgagatcggc cttgatgggc gtcagcagcg
     3361 aggagatgat catcgcatcc accacgcaga caatgcccag cagcatgatc gccgccagcg
     3421 aaacgtgagg ggatgcgggg cgcgccgccg catggctttc ggcagaacca gcctgccagg
     3481 gcgcctgagt gagggaggga acaggcaaaa cgaatctcca ctgattagat agactttatg
     3541 gactactatt aatgccagta accagataga aattattgga acatgcaagg aaggctttgc
     3601 ccacccccac tccgccccca tcaaatcctg cgcttttcca tggccttccc cgcccccgca
     3661 cggcataagg cgcggcatcc ggaagggagt gcgcgatgaa ggtgggaatt gtgggcgcgg
     3721 gcatggtcgg ctcgatggcg gcctatgcga tggcgctgca gggcacggcg gccgagatcg
     3781 tgctggtcga tcgcaacgcc gatctggccg ccgcccaggc cgaggatatc agccatgcca
     3841 cccccttcct ccagccgcgg cgcatcgtgg cgggcgatta tgcggcgctg gcaggcgcgg
     3901 gcgtggtgat cctggccgcc ggcgtgccgc aaaagccggg cgaaagccgg ctcagcctgc
     3961 tgtcgcgcaa tgccgatgtg tttgcagaag tggtgggcca ggtgcgcgcc gcagcccccg
     4021 aggcgctgct ggtggtggcc tccaacccgg tggacatcat gacgatggtg gcccagcgtg
     4081 cctccggcct gccgcccgaa cgggtgatcg gctctggcac gctgctcgac accgcccgct
     4141 tccgccagtt gctggcatca cggctggggg tggcggcgca atcgatccac gcctcggtgc
     4201 tgggcgaaca tggcgacagc gaagtgctgg cgtggtcgtc tgcccaggtg ggcggcaagc
     4261 cgctggaacc gggcgctgcc ctgctgggcg cgccgatcac cgccgaagtg cgcgccgcca
     4321 tagacgaagg cgtgcgccgc gcggcctatc gcatcattgc cggcaagggg gccacctggt
     4381 tcggcattgg cgcggggctg gcgcgcatcg tccaggccat ccgcgacgac cagcgcgcgg
     4441 tgctcaccgt ttccatccgc atgcccgagg tgctgggggt ggccgatgta gcgctcagcc
     4501 tgccgcgcgt ggtggggcgc gagggcgtgc tggcaacgct gtggcccgat ctggcgccag
     4561 acgaggaagc ggccctgcgc gcctcggccg aagtgctgca ggccgccgcc gggggcctct
     4621 ggcgctaggc gctaggcgct caccagcttg tcagccggcg cagctggggg gttttcttgt
     4681 gcacatggtc gtccatgcgc gccagcagcc cgtccagtgc ggcaatcgcc tcgaacagga
     4741 aggcgccctt gttcagcatc acgcattcgg cgcgggcggc catggcggca tcggtcagct
     4801 cgccgcggtt gggcagccct tccttgacga gatgttccag cacctgggtg gcccagatca
     4861 ccggcacctg cgccgcctcg gcgatccaca ggatctcctc ctgcatttcg gccaggcggg
     4921 caaagccgat ctcgatggcc agatcccccc gcgcgatcat gatcgccacc ggctgacggg
     4981 cggccgcggc cagcaggata tcgggcaggt tgtgcaccgc gcgcgcggtc tcgatcttga
     5041 ggatcagcgc cagttcctgc cagtcgtccg ggcgcagccg cgccagttcg gcctgcagca
     5101 gttcgacatc ggccacttcc tgcacgaagg agaattccac cccgtcggca tgggcggcca
     5161 caaaggcgag atcggcgcga tccttggcgg tgagcgccgg cacctgcaaa tcggtatcgg
     5221 gcaggttgat gcctttttcc ggcttcagct tggccccctc ctccttgcag cgggtgacag
     5281 aggcgatcag catcccctcg cccaccatct cgatcgcgca ggcgatcttg ccatcatcga
     5341 tcagcaggcg ctcgccgggg gccgccgcgg caatcgcctc gggcagcgtg cattccaccg
     5401 tgcaggcgac atcggccggc accttgcgat ccagcccgcc cggcgccacc agcgccagcc
     5461 tgtcgcccgg gaacaggcgc ttcacatcgt ggtgatggcg cagcccgccg gtgcgcagct
     5521 tggggccggc cagatccatg aagacatggg ccgcctgccc cccttcgccc gcgcgcagat
     5581 gggcgatcat ccgcgcccag gcctcgggcc cgtcatgcgc gcaattgatg cggatggcct
     5641 ccaccccgcg cgcggccaga tcgcgcatga aggccggatc atcagccgcc tcggacgggc
     5701 aggtgaccat cagcgcggcc tgccgctgct ggcgccgcgc gccaaacagc tggcgcgcgc
     5761 gggcctccag ccgctgttcg ccggcgaaga aatcctcgcc tcccacagct tctgccggct
     5821 gcccggccat ccctgccagc gccgcgcgca ccgccagcaa ggtgggcatc acccggcttt
     5881 ccagccgccc cagcgatgac aggcccagca ccatcagccg gcgctgcagc gggcgcagat
     5941 cacggtggcg cagggccaga taggcggcca ggttttccgc gctgggcagg aaggcggggc
     6001 ggatgccgcc tgcgcgccac agttccatgc gctggcgcgt ctcgctgcgc acggcggcga
     6061 tcagcgcgtc cagctcatcc agcagcgccg cgtgggttgt cctgcctgcg ccggcgatgg
     6121 cggggcgttc ggcagccatg ggatcgcctg tcacacatgc tccgtcatat ctggccttga
     6181 tgccgttttt atccttatgc cctgcctagg cggcctttgc gatatttgcg tgacaggatc
     6241 tgcccgatgg cgccgcctgt ttacgaaagc tttatgccgg cgcccgcctt tccactggaa
     6301 gcttcacggc gatcggcgcc attccggcgg gcatggcaca ggtcatttcc caaaggctgc
     6361 gcgacgggcg cccgctctgg cacgagcaac tggccttccg cctggccggg ctgggcgggc
     6421 tgtgggggtt tgcgctgttc gcacgctggc tgttctggct gcagcagcgc gcgcccgccg
     6481 tggccggccg gccctgcatg ctggccggct gcctggtggc ggtggccgtg ctgcaggcgg
     6541 ggctggcgct ggcactggcg gggccgggcc tgctgcgcca ggtgccctgg ccggggatcc
     6601 gccgcatcga gccgggaaca cgccgcatcg ggccgggcgg cccctctcct ctccaccgcc
     6661 cggggccgca tgcatgacag cgtgatgatc cgggccagac cgacgttcct tcctgtgaac
     6721 gcaactcaag aggccggagc gcactgctcc ggccttcctt ttgcgcgctt tcgccgtgct
     6781 tttcgccacc aaagcggatg acaccatact gtcaccggcc tagggcattc ggtcaggctc
     6841 cttgggcatg gcacggcacg gatacgcata aagatggacg gcgaaagaag ggttccctgg
     6901 ctgggcatga tggtggcgct gggaatcagc gggtggctga tgctgtcggg cgtgaacacc
     6961 atgtcgattg cgctgctggg gccggtgtgg ctgctctcgc tgtggctcga ttggctgcgc
     7021 ccggtggccc gcccggcgat ccgcatcgcc atcaaccgcc ccgatatcga cgcgctgacc
     7081 cgcgaggcga tgacccgcac gctggaaggc gtgagcctgc cgctggtgct gatggagcgc
     7141 caccgcatca tggccgccaa tggcgcggcg cgggcggcct ttggcgcgca tatcgtgggg
     7201 caggatgcgc gcattgccct gcgccacccc gaagcgatgc ggctgctcga catgccttcg
     7261 ggcggcagcg tgacgatccg cgggctgacc ggcaccggca gcctgtggca gctgacccgc
     7321 cagacgatcg attcccgctt ctggacgatc gagctgcgcg accgcaccac cgaggcggat
     7381 ctgggccgcg cccacaccga tttcgtcgcc aatgccagcc acgagctgcg cacgccgctg
     7441 tcttccatca tcggctatgt ggaaaccatt gccgatgatc ccggccggat ggaaccggac
     7501 accacccgcc gcttcctggg caccgtgctg cgcgaggcac ggcggatgca gaacctggtc
     7561 tccgatctca tgtcgctgtc gcagctggag gcggaaaagc acgacctgcc ttccgagcgg
     7621 gtggatctgg ggcgcctggc gcatcgggtg gtgggcgaat ttgcccccgg cccccagggc
     7681 agcggcccgc gcctgaggct gagccggccc gacgagacga tcagcgtggc gggcgatgtg
     7741 aagcagctgg agcaattgct gcgcaacctc atcgacaatg cgctgaaata tgccgatggc
     7801 agccagccgg tggaagtgag cctggccggc gccccgcgcg gcatggtgga actggtggtg
     7861 gccgatcatg gccagggcat cgcgccggaa cacctgcccc acctcacccg ccgcttctac
     7921 cgcaccgatc cgggccgcag ccgggcggcc ggcggcaccg ggctgggcct ggccatcgtc
     7981 aagcatatcg tcgagcggca ccgcggccgg ctggatatca ccagccagct gggcgtgggc
     8041 acgcaggtgc atgtgcgcct gccgctggca gcggccccaa gctgacaccg ctggcagcgg
     8101 cccagagctg acgctgctga ctgtggccca aaaccaccgc tgcttgcggc gaaccgccaa
     8161 ctgtcacatt gctgtcatgc taggccgcca tggcgctgtt tcatgaggca tcgcgcaggc
     8221 tgcgagcccg ctgccagcat ggcaaagaca cgctggcgta acagtttggc ttgtagaagg
     8281 ccgaagtgac aaggtaacca aagacgccgc atcaggcggc gacgcgcagg cttgaggtag
     8341 ggaacatggt tgcagaacac acggtcaagg ccttcgacga ggaaatcacc agcctgcgcg
     8401 gactgatcgc cgaaatgggc ggcctggccg aagtggccat cagcgaatcg atggatgcgc
     8461 tgacccatgg ctcgctgcag ctggccgagg aagtgatcgc ccgcgacaag cgcatcgacg
     8521 cgctggaaac gcaggtggaa cagctggcgg tgcgcatcat cgccctgcgc gcccccatgg
     8581 ccgacgacct gcgcgacatc atcgccgcgc tgaagatcgc cggcattctg gagcgcatcg
     8641 gcgattatgc caagaacatc gccaagcgcg tctcgcggat cgagcggcgc gaggtgttcg
     8701 agccgctgac gctgatcccc gccatggctg aagtcgccgc cgaaatggtg catgacgtgc
     8761 tgaccgcctt tgccgcccgc gacccgcagc tggcgatcga cgtggtggag cgcgatgcca
     8821 aggtcgatgc cttctacgac agcatcttcc gcaacatggt gagcttcatg atggagaacc
     8881 cctcgaccat caccgcggcc gcccacctgc tgttcgtcgc ccgcaatctg gagcgcattg
     8941 gcgatcatgc caccaatgtc gccgagcagg tctattacgc cgccaccggc gaatatccgg
     9001 ccgagcgcac cgagcgcccc gagaattgat cctgactgac acctctttcg ggatctgctg
     9061 ccgatgaatc cgccgcgcct gcttctggtt gaagacgacc cagcccttgc cgaactgctg
     9121 gaattccgct tccgcgcgga aggctacacg gtttcggcca cggccgatgg ggatgaagcc
     9181 ctgctgcgcg cggcggaaga ggcgcccgac cttgtcgtgc tggactggat gatcgagggc
     9241 accagcggta tcgaagtgtg ccgccggctg cgccgcgaca aggccaccgc ccatgtgccg
     9301 atcatcatgc tgaccgcgcg cggcgccgag gacgacatga tccgcgggct ggaaaccggc
     9361 gccgacgatt acatcaccaa gcccttctcc ccgcgtgagc tgatcgcccg ggtggcggcg
     9421 atcatgcggc gcatccgccc ggcgctggcc ggcgaatcgc tgaccgtggg cgacctgacg
     9481 ctggacgcca cggcccaccg cgtgacgcgc cggggccaga tggtgcagct gggccccacc
     9541 gaatttcgcc tgctgaaatt cttcatggag catccgggcc gggtgttcag ccgcgggcaa
     9601 ttgctggatg ccgtgtgggg caccgacagc gatatcgaac tgcgcacggt ggacgtgcat
     9661 atccgccgcc tgcgcaaggg catagaagtg gatggcgcgc ccgatccggt gcgcacggtc
     9721 cgctcggcgg gctatgcgct ggaagcggtg taggcacagc gccagtgaag atgtgaaaag
     9781 gggggccggc cagcccccct ttttcatgcg ccggcgcggc tcagaattcc ggtgcgggcg
     9841 gcaccggcag gccgggcgcg tcctcgtcca cctcatgcac ttccaccagc ccgcggccga
     9901 catggctgtg cgaacggctg tagccgaaat agaccaccag gccgatcgcc gcccaggccg
     9961 gcagcaccag catcgcggcc agcggcaggt tgaagaacag gaacacgcag ccgaaaatcg
    10021 ccgccggggc gatcagccac accagcgggg tgcggaaagg gcggtggcgg ttgggctcgg
    10081 tccggcgcag gatcagcacg gcgatcgcca ccatcatgaa agcatagagc gtgccggcat
    10141 tggcaatgtc cgacagttcg cccaccggga agaaggccgc gcccagcgcc gttgcaaggc
    10201 cggtcagcgc ggtgacgata tagggcgtct tccatttggg atgcacgcgt gagagcgcct
    10261 cgggcagcag gccatcgcgc gccatcacga agaagatgcg ggtttgcccg aagagcagga
    10321 tcagcaccac cgacggcagc gcgataaagg cggcaatgcc catgaaattg cccaccgcgc
    10381 caaagccgat cacatgcagc acatgggcca gcgcctcatg gctgcacacc agccggtcgg
    10441 catattgcgg caggatgcat tggctggcca gttccggcga tccggccggc agcggcgtgc
    10501 catcgccgcc catgatcggc tgcccgccaa tcgaaccgac agcgcccgct gccaccgcca
    10561 tgtagaacaa ggtgcagatc agcaggctgc ccaccaggcc gatcggcacg ttgcgctggg
    10621 ggtcgcgggt ttcctccgcg gcggtggaaa cggcatcgaa gcccacatag gcaaagaaga
    10681 tggtggccgc cgcccccacc gcgcccacgc ccgtgccaaa gccgccaaac acgccggccg
    10741 gcaggaaggg gctgaaattg tcgccatgca tgtgcggcgc ggtgagcacc acgaaaacgg
    10801 tgagcgcggc gatcttgatc gccaccagaa aggcgttgaa acgcgcgctt tccttggtgc
    10861 ccagcatcag caggccagtg atcagcagcg agatcaccgt ggccggcagg ttgatgaaac
    10921 cgcccggcgc cccccccagc accagcggcc ccgcgctgag cgctgtgggc aggtgcacgc
    10981 ccattgtctt gccaaggatg gtgccggtga aatagcccga ccagcccacc gccaccgccg
    11041 aggccgcgat cgcatattca aggatcagcg cccagcccac cgtccaggcc agcaattcgc
    11101 ccatcaccgc ataggtatag gtgtaggcag agccggccac cggcagcatc gcggcgatct
    11161 cggcatagca cagcgccgcc accacgcaga tcaccccggc aatcgcgaaa gccagcatca
    11221 ggcccggccc cgccttctgc gcgcccgccg ccgtgagcac gaagataccg gtgccgatca
    11281 cacagccgat gccaagcagg gtcagttgca ccgcccctag cgaacgatgc aaagatttcc
    11341 gttccgccgt tgccaaaatg gcatcgagcg gcttcacgcg accgaacatg taattcccct
    11401 gaaccgttgg cggctaaggg cgggcaggct aacgcaccgg tggcgcttgg caagggccta
    11461 aggcgggcaa atccaccggt ccaacgcgga aaaacggcca tattcggcct tgcgccctct
    11521 ccttttcgcg aatggctcgc atcaatgctt tgccttgccc ggccaagggg ctagaagcgg
    11581 gcccatgtct accaccttcc agatcgacac cgccacctcg cgcgccaatc ccaccccggc
    11641 gccgatgaag cgcctgacca tccctgccct gcgcgcccgc aaggccggcg gccacacggc
    11701 agaaccgatc gtgatgctga ccgcctatac cgcgcggcag gcccagctgc tggatccgca
    11761 ttgcgacctg ctgctggtgg gcgattcgct ggggcaggtg atctatgggc tgccctcctc
    11821 gctgccggtc acgctggaga tgatggccgc ccatggcgcg gcggtggtgc gcggcagctg
    11881 gcatgcggtg gtcgtggtcg acatgccctt tggcagctac gagcaatccc cggcccaggc
    11941 cttcgagaat gccgcccgcc tgctgaagga aaccggcgct gccggcgtga agctggaagg
    12001 cggcgcggcg atggccgaaa cggtggcttt cctttcggcc cgcggcattc cggtgatggg
    12061 ccatgtgggg ctgaccccgc aggcggtgaa ccagctgggc ggctatggcg cgcgcgggcg
    12121 cagcgcggca gaagccgaga agatcctcgc cgatgccagg gcgctggatg aggccggcgc
    12181 ctttgccgtg gtggtggaag gggtggtgga gcccatcgcc attgccgtgc agcaggcgct
    12241 ggcctgcccg gtgatcggca tcggcggctc tgcccagtgc gatgggcagg tgctggtggc
    12301 cgaagacatg ctgggcctgt tcgaacgcgt gccccgcttc gtgaagcgct acgaggacat
    12361 ggccgggcgg atctcggaag cggtggccag ctatgccgcc gatgtgcgcg cgcgcagctt
    12421 ccccggcgcc gaacagacct accagcccaa gcgctgaggc cgcgccatgt ccctcgctgc
    12481 ggccctgcgc cggcatctgc tgggccctgc cctgttcacc cttttgtgcc tggcgctggc
    12541 cggcgcctat agctggcagg ccagcccttc tgtgcccctg ctgctggggc agctgtgggt
    12601 ggtggtggtg ctggcggtgc tggaagtctc gctcagcttc gacaatgcgg tggtgaacgc
    12661 ggccctgctg gccgatatgg acgcggtgtg gcgcaagcgc ttcctcacct ggggcatggt
    12721 gtttgccgtg tttggcacgc gcatcctgtt tccgctggcg atcgtgggcc tgtcggccgg
    12781 gctggatccg gtggcggcgg tgcgcctctc gctgacagag ccggcgcgtt acgaggagat
    12841 cgtgaccgcc gcccatctgc cgattgccgg ctttggcggg gcgtttctgg cgctggtggg
    12901 gctgcgcttc ttcctcgatc agggcaagca gcggcactgg ctgcgctggc tggagcggcc
    12961 gctgcacggg ctgggccggg tggaagcggc cgccatcgcg ctggtgctgg ccgccctgct
    13021 ggccggcggc gcctggctgg ccccgcaaga cgcccatggc tggctgaccg gcggcatcgt
    13081 tggcatcatc actttcgtgc tggtggaagc gctgggcaat ctgctgggcg cgggcgatgg
    13141 cggccatggc gccacggtgg cggtggcccg ttccgggctg ggcgcctttc tctatctcaa
    13201 cgtgctggat gcctccttca gccttgatgg ggtgattggc gcctttgcgc tgtcgaacaa
    13261 catggtgatc atcgcgctgg gcctcagcat tggcgcggct ttcgtgcgcg cgctcacgct
    13321 catcctggtg gagcgcgacg tgctgggcca gctggtgttc ctggagcatg gggcgttctg
    13381 ggccattctg gcgctggcgg gcatcatgct gtcggcgccg ctggtggaag tgccggagag
    13441 catcaccggg ctggtcggcg cggggctgat cgctgtggct ttctggcaat ccttgcggct
    13501 gcggcagcgg ggcggcgcat aggcccctgc atgggccggc gcataggccc ctgcataggt
    13561 atgcctgatt gtaccgtttc gttcacgaac cctgaagaag tgcctatgtg gttatctggc
    13621 aacggttgct gccaatttgg gcccaatccg caattccctg ttgtgaaggc cgcttcttta
    13681 tgacagttga tgtctcggaa gaaaaaggga gaggagaagg gcgtccattg ggcgcccttt
    13741 ttcatttccg gcaaaaaccc cgcccgcccg ggctttgcag ggccagcggc ggcccctttc
    13801 agccctgcat atcatattgt ttcaacaggt cgtagagcgt gggccggctg atgcccagca
    13861 gccgcgcggt gttcgagata ttgccttcgc tgcgagcgag ggcgtggcga atcacccgcc
    13921 ggtcggcctg ttcgcgcgcg gctttgaggt tgagcaattg tgcgtctggt tcctgcggcg
    13981 cggccaggtc gagatcggcc gccgtcacca gcttgccctc agccatgatg gcggcgcgct
    14041 tcacccggtt ttccagctcg cgcacattgc ccggccagct ccagctgtcg atcgcggcca
    14101 gcgcatcggg ggcaaagccc ttcacctgcg ggttcagctc acgcgcgaag cggctgagga
    14161 aagcgcgcgc cagcagcggc ggatcgcccg cccgctgggc cagcgccgga atgcgcacga
    14221 cgatctcggc caggcgatac cacag
//
LOCUS       DASRYE010000281         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_211687,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000281 DASRYE010000000
VERSION     DASRYE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211687"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFF98_14440"
     CDS             complement(<1..430)
                     /locus_tag="VFF98_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU64880.1"
                     /translation="MDVYLPIANLAVNGLVIIGLGALTGVLSGMFGVGGGFLTTPMLI
                     FYGIPPTVAAASAASQVTGASVSGVFAHSRRGGVDYQMGAVLVAGGVLGTGAGSVLFT
                     FLQRLGQIDTVINILYVLLLGGIGGLMAKESLSTLAATRRG"
     gene            complement(492..1781)
                     /locus_tag="VFF98_14445"
     CDS             complement(492..1781)
                     /locus_tag="VFF98_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64881.1"
                     /translation="MTAQSVARGQPLWVFLGLTMVWTAGRALLWQAPAFEAAQGPGQR
                     LAHDWGLAAAPALLPAPLPPAPLPLAGLVTAGPLCLAVIPGGQVVAMMAPPAYGAAYG
                     AAYGGALAPLAAGGCAPAVARLADGGGTGRGRAGGGASAAEPFAYAGDEGYRLAPAAH
                     ARAPEAQLAGGSALEAEVLARAGAGMVMLSGGADAPPAAQPAASAPAPAARPRRFGGD
                     AWLLWRPGGTFNGASAPMATYGASQVGSVLRYRLAPDSAPSLALYLRGTAALNGSLER
                     DMAAGLQLRPIAGLPLLAMAEVRNSLFYDGTVHVRPAGMVVAQPRQASLPLGFKSTLY
                     VQGGYVGGAAATPFVDGQWKVEHPFDAFGKGNLRLGGGLWGGAQTGAGRLDVGPSVSI
                     GAHRGRLGLRLGADWRMRVAGHAAPGNGPAVTLFGGF"
     gene            complement(1778..>2878)
                     /locus_tag="VFF98_14450"
     CDS             complement(1778..>2878)
                     /locus_tag="VFF98_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HZU64882.1"
                     /translation="AARAAGGDGRVRLVIHDRAGPTTKADCLNRLYRALAQDEARTRR
                     PYRGVLLHDAEDMVHPLALAVIDQALETADFVQLPVRPEFPPGPRWVSGHYCDEFVES
                     HAKAMVVRSALGAGLPAAGVGCAFSRVMLEKVAHERGAGGNPFAAECLTEDYELGLLI
                     ARLKGRSRFLRLRDGSGQLIGTRSYFPATLPAAVRQKTRWIHGISLQSWERLGWSRGL
                     ADIWMALRDRRGPLTALVLTCAYVLMLIEAGMLLASLFVPTPMLRYSPAVLGLMRLGL
                     AGFFWRAAMRFAFSAREYGLVEGCFAVLRIPVANVITIMAGRRAFVAYCRSLRGMRVA
                     WEKTVHINHPADHPVRLLRQMAPPAAGLGQAA"
BASE COUNT          486 a         1109 c          965 g          318 t
ORIGIN      
        1 cgccgcggcg ggtggcggcg agggtcgaaa ggctttcctt ggccatcagc ccgccaatgc
       61 cgcccagcag cagcacatag aggatgttga tcaccgtatc gatctggccg agccgctgca
      121 ggaaggtgaa cagcaccgag ccggcgcccg tgcccagcac gccgccggcc accagcaccg
      181 cgcccatctg ataatccacc ccgccgcgcc ggctgtgggc aaacacgccc gaaacgctgg
      241 cccctgtcac ctggctggcg gcagaggcgg cggccaccgt gggcggaatg ccatagaaga
      301 tcagcatcgg cgtggtgaga aagcccccgc ccacgccaaa catgcccgac agcaccccgg
      361 tgagcgcgcc aaggccgatg atgaccagcc cgttcacggc cagattggcg ataggcagat
      421 aaacatccat gctgccggcc agactagccc gccgcccgcc cgctgggaag ggtgcgcgaa
      481 gggcgtgccg ctcagaatcc gccgaacagc gtgaccgccg ggccattgcc gggcgcggca
      541 tggccggcca cgcgcatccg ccagtctgcc cccagcctca ggcccagccg cccgcgatgg
      601 gcgccgatgc tgacagaggg gccgacatcg agcctgcccg cccccgtctg cgcgccgccc
      661 cacaggccgc cgcccagcct gaggttgccc ttgccgaaag cgtcgaaggg gtgttccacc
      721 ttccattgcc catcgacaaa aggcgttgcc gccgccccgc cgacatagcc gccctgcaca
      781 tacagcgtgc tcttgaagcc cagcggcagg ctggcctggc gcggctgggc caccaccatg
      841 ccggccgggc gcacatgcac cgtgccatcg tagaacaggc tgttgcgcac ttcggccatg
      901 gccagcagcg gcaggccggc gatggggcgc agctgcaggc cggcggccat gtcgcgctcc
      961 agcgagccgt tgagcgcggc cgtgccccgc agatagagcg ccagcgaggg cgcgctgtcg
     1021 ggcgccagcc gatagcgcag cacgctgccc acctggctgg cgccataggt ggccatgggc
     1081 gcgctggcgc cattgaacgt gccgcccggg cgccacagca gccaggcatc gccgccaaag
     1141 cggcgggggc gggcggcagg cgcgggcgca ctggcggcag gttgtgccgc cggcggcgca
     1201 tcggccccgc cagagagcat caccatgccc gccccggcgc gggccagcac ctcggcctcc
     1261 agcgcggagc cgccggccag ttgcgcctct ggcgcgcgcg catgggccgc gggcgccagc
     1321 cgatagcctt catctccggc ataggcgaaa ggctcggcag cggaggcccc gccgcccgcc
     1381 cggccccggc cggtgccccc gccatcggcc aggcgcgcca cggccggcgc gcagccacct
     1441 gccgccagcg gggcaagggc cccgccatag gccgcgccat aagccgcgcc ataggcaggt
     1501 ggggccatca tcgccaccac ctgcccgccc gggatcaccg ccaggcacag cggcccggcc
     1561 gtcaccagcc cggccagcgg caagggggcg ggcggcaaag gagcgggcag caaggccggt
     1621 gccgctgcca gcccccaatc atgcgccagc cgctggccgg ggccctgcgc tgcctcgaag
     1681 gcgggggcct gccagagcag cgcccgcccc gccgtccaca ccatggtgag gcccagaaac
     1741 acccatagcg gctggccgcg ggccacgctc tgggccgtca tgccgcctgc cccaggccgg
     1801 cggcaggggg cgccatctgc cgcagcaggc gcacgggatg atcggccgga tggttgatat
     1861 gcacggtctt ctcccacgcc acgcgcatgc cccgcaagga gcggcaatag gccacgaacg
     1921 cccgccgccc ggccatgatg gtgatgacat tggccaccgg aatgcgcagc accgcaaagc
     1981 agccttccac cagcccgtat tcccgcgcgg aaaaggcaaa acgcatcgcc gcgcgccaga
     2041 agaagcccgc cagccccagc cgcatcagcc ccagcacagc cggcgaatag cgcagcatgg
     2101 gcgtgggcac gaacaggctg gccagcagca tgcccgcctc gatcagcatc agcacatagg
     2161 cgcaggtgag caccagcgcg gtgagcggcc cgcgcctgtc gcgcagcgcc atccagatat
     2221 cggccagccc ccgcgaccag cccagccgtt cccagctttg cagcgaaatg ccatggatcc
     2281 agcgggtttt ctgacgcacg gcggccggca gcgtggcggg aaaatagctg cgcgtgccga
     2341 tcagctggcc gctgccatcg cgcaggcgca ggaaccggct gcgccccttc agccgcgcga
     2401 tcagcaggcc cagctcgtaa tcctcggtca ggcattcggc ggcgaacggg ttgccgccgg
     2461 cgccgcgttc atgggccacc ttttccagca tcacgcggga gaaggcgcag cccaccccgg
     2521 ctgccggcaa accggccccc agcgccgagc gcaccaccat cgccttggca tgcgattcaa
     2581 caaattcgtc gcaataatgg cccgacaccc agcgcggccc cggcggaaat tccggccgca
     2641 ccggcaattg cacgaaatcg gccgtttcca gcgcctggtc gatcaccgcc agcgccagcg
     2701 gatgcaccat atcctccgca tcatgcagca gcacgccgcg atagggccgg cgcgtgcggg
     2761 cctcatcctg ggccagcgcg cgatagagcc ggttgaggca atcggccttg gtggtgggcc
     2821 cggcgcgatc atggatcacc agccgcaccc ggccatcgcc gcccgcggcc cgggccgc
//
LOCUS       DASRYE010000282        15304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_168710,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000282 DASRYE010000000
VERSION     DASRYE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168710"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..976)
                     /gene="epsC"
                     /locus_tag="VFF98_14455"
     CDS             complement(50..976)
                     /gene="epsC"
                     /locus_tag="VFF98_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZU64883.1"
                     /translation="MDLADIAAALRQRRAAWRATREREHLRDPAYGAHGFPARHALGR
                     IVDTLAAALFPMRLGPPELTLANEDAFVEASLGSALPALAAQLRMELAALGPAEAATA
                     ADAGATRMVRKLAARLPDIRRLLDSDVEAAFAGDPAARSVDEVLLCYPSFTALIHHRI
                     AHEIYKLGAPLVARIIAEIAHSHTGIDIHPGATIGESFFIDHGTGVVIGQTAIIGRRV
                     RLYQAVTLGARSFTVDEDGHLLNTPRHPIIEDDVTIYAGATVLGRITIGAGSVIGGNV
                     WLTRSVPPRSQVRQSKPSVIIETDPLADWPDL"
     gene            1288..2187
                     /locus_tag="VFF98_14460"
     CDS             1288..2187
                     /locus_tag="VFF98_14460"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZU64884.1"
                     /translation="MAVLSWVMMMAMPVGPVGPVLPAVVEPPAALAQPLAAGAPAGAG
                     PEGASPPPTTPPPAAQPDAAAQADAPVPASAPPPASDDMLATPRDPWQKTNRTLFAIN
                     NDVDHVLIAPVAHAYVFVFPRFVRHRIGNVVGNMNEPMVAINNLLQGHPGRFVHTLSR
                     FALNSTIGLGGMFDVVAWKLPHRDADFGQTLGRWGAKPGPYVVLPFFGPSDLRDGFGR
                     AVDIAADPVGWVFGDFLTTFGASRLGAEIVHGRAEAEPAIRALRDSTDPYATARSGYI
                     QARAAILRDTTGKAEALPDFDAP"
     gene            2297..2923
                     /locus_tag="VFF98_14465"
     CDS             2297..2923
                     /locus_tag="VFF98_14465"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU64885.1"
                     /translation="MALLRRVGAPLALGMGAAAALVPAAAMAQARDAQAEAYVDANAR
                     RVLGVLNDHHLSVEGRRQAFHTLIGELVDWPRLTRFVLGKYARTATPDQFQRFAAAYR
                     TYEEGVYQRRIDDYKGERAVVSGSLIRKPGDVIVTTLLSGGQSPQPTALSWRVLGGGA
                     GWKVVDVEVKGVWLAITQQQDFVSTIDNAGGKIDVLTAQLARNEAPRR"
     gene            2999..3532
                     /gene="mlaD"
                     /locus_tag="VFF98_14470"
     CDS             2999..3532
                     /gene="mlaD"
                     /locus_tag="VFF98_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZU64886.1"
                     /translation="MRAHWGETAMGFVVLALAGLFLVYSLGVAHAGRSGQGYELTARV
                     GDSGGLAPGAKVTIGGVKIGSVTAVTLDPKSYLAVIHLSVDSTVPLPTDSTAKITTDG
                     LLGGAHVALTPGGAQGNLKAGDEIANTQGAVDLIGLIGQFIRPHDDAPAAGAASAPAA
                     AANAAAAKPAPYPAGVD"
     gene            3534..4349
                     /locus_tag="VFF98_14475"
     CDS             3534..4349
                     /locus_tag="VFF98_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZU64887.1"
                     /translation="MTGSDVPESPPPRLLAPVAALGRATLAGAGSIGQVALFALAALR
                     AALTPRWFGGQIGRQLWQVGFLSLPVVGMTIWFTGAALALNVWTGGSRFNAEQAMPQI
                     VAIGITRELGPVLAALMLAGRVAAAMASEIGAMRATEQIDAMTTLSTDPMRYLIAPRL
                     LAGVLSMPLLTLVANCFGIAGGLVVAVQVLDIPPPTYIQNTLDYLQDWDVESGLVKAA
                     VFGFIVALMGCYHGMNARGGARGVGRAATQAVVSSAILILAADYLLTSLFSRM"
     gene            4358..5134
                     /locus_tag="VFF98_14480"
     CDS             4358..5134
                     /locus_tag="VFF98_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZU64888.1"
                     /translation="MPVETPLPKLAWKNVSKRFDGPPVLAGLDLAVAAGRSLVIIGGS
                     GQGKSVAIKVALGLIPVDGGEVLLDGVNIAGLDAAGRRQALARVGMLFQGGALFDSLS
                     VWENVAFRLINAEGVPRREARARAIDTLALVRLGPQTADLYPSELSGGMQKRVGLARA
                     IIGAPEILFFDEPTTGLDPITAAAINALIVDRVRSLGCTAVSITHDLASARTIADEIA
                     MLHHGRIVWRGLAAQLDSTDNPYVRQFVDGRTDGPINADR"
     gene            5201..5539
                     /locus_tag="VFF98_14485"
     CDS             5201..5539
                     /locus_tag="VFF98_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64889.1"
                     /translation="MARKHSRRDPYGDLDREEEAPVEVVPCWLCGRPTGQTIVWHHPV
                     PKSRGGRDVVPMHPICQQTLIANFTNSELQRHGMDVEALLAHPAVRKFVDWVANKDPD
                     FTATTAKKQR"
     gene            complement(5555..6148)
                     /locus_tag="VFF98_14490"
     CDS             complement(5555..6148)
                     /locus_tag="VFF98_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64890.1"
                     /translation="MARPKSPLISLDLVITAATQILESEGLDALSLRHLAARMQVNSA
                     SLYHHFANKDEILLAVVRKALRDVQILPPTDDWAQWIAGNGVHYRRLLVAKPFLVPLL
                     LNGIRPPTMTYALSDQKLADIHLPAALRHEFLIALDNAVIGSALISIKASQFNGGIAD
                     THFDHEALLRHTIRAMIAEMIDQFRKMASPPPARAQL"
     gene            6381..7550
                     /locus_tag="VFF98_14495"
     CDS             6381..7550
                     /locus_tag="VFF98_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64891.1"
                     /translation="MAGMDTNVALDTSVVAQLIEHIRNKSTDRAEQELRVPASQFTCP
                     DRARAEIALLKTCPLVIGHASEVPQAGDFITRDLLGASVIVSRGKDGRIATFRNMCPH
                     RGGRVEQKEAGRKHIFMCQYHGWSFNAEDGALRPVPYEDSFGPIDYACSGLQRIRTEV
                     RHGLIFVDLSDNCERDIASFLGAEVEAQLAPWQLDQSRVYLDKSFTVPINWKLVMDGA
                     VDSLHAQYLHPGPGNVGARTLTNIAVFRAFGPHGRLFVPRTRMKKLVDQGEEAAATTN
                     YVASIMLLYPNALMAAAPDHVEFWTVWPSANPAECTVRIRFFVRPEILSPEMEERIDR
                     SWAILENAATTEDWPMEVWIQENAMAWPDTAFRYGRNEVSAQHLHHELIRALAAS"
     gene            7674..8879
                     /locus_tag="VFF98_14500"
     CDS             7674..8879
                     /locus_tag="VFF98_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU64892.1"
                     /translation="MAGPDQSGSGQSLESFDIHAPDARVGAECLWAQMRAQPGLYHSD
                     RYGGFHVAARFEDVMAVLMKPALFSSARGITLPPPTAVRSLHIPAEVDPPLHGEYRAL
                     LMPFVTPEQARLREPAIRALVRQLLARFPDGQPVDFVRLLGRPLPILVALDLLGMPGE
                     DAEALETLVGEIHRDVAAGVSSGAIDRLQAYAERVLERRRAMASDPGEDLVSSVLLGQ
                     VQGRPLTGEEQMSIIRLLLIGGFDTTSATLSEMMRWLAETPEAAARLRADPALIPAAV
                     EEMVRVASPSTYLRREVTQDCELGGTPLKEGDSVLVAFGAANRDPAKFACPETVDPAR
                     KPNPHVGFGAGHHRCIGSFIAKVELKVAFEEILAHFADFRLDGPVRYTSGLGQGLISL
                     PMVFARAAR"
     gene            8909..9715
                     /locus_tag="VFF98_14505"
     CDS             8909..9715
                     /locus_tag="VFF98_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU64893.1"
                     /translation="MPAIEPGRYEWIKVARGEDGIATLTLNNPRRKNALARPMHRELE
                     RIWDDIDADDDIRVTILTGEGDAFCAGTDLSFQDGENSQGRKGRPPTRGARRLFWNML
                     DSEKPIIAKVRGPAYGVGVNIALACDMVIAAEGARFCDSHVRMGIAPGDGGVAFFPLL
                     LGFHRAKEYLMLGEPIEARKAAEIGLINYCVADAELDALVDSYAQRLANGAPLAIAYG
                     KMSVNLLLKQMTAGAFETSLAYDQLTLFTEDHKEGARAFLEKRSPRFTGS"
     gene            complement(9765..10793)
                     /locus_tag="VFF98_14510"
     CDS             complement(9765..10793)
                     /locus_tag="VFF98_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU64894.1"
                     /translation="MEQRFLGRSGLQVSVIGFGTMTFGGGESGGKFAAIGTTDLAQAK
                     RQVALALDHGVTLFDTADNYADGHAESLLGAALGTRRRDVVIATKAFGRTGPSTHDIG
                     LSRRHLVSACEASLRRLGTDWIDLYQVHNHDGLVPMEETLRALDDLVRAGKVRHIGCS
                     NHFAWQLAKAIGLSARHGLESYVSQQILYSLAYRQAEQELLPAALDHGVGTLVYSPLA
                     QGYLSGKFADAGSEGRLLATRQFAGTDTPHARGIVAELARLAASAGPGRTPSQLALAW
                     LIGRPGISAVIVGARSESQLRENLAAATTTLAAAERTALEAASAIAPWYPRTAQRVFH
                     PERNPPID"
     gene            complement(10815..11615)
                     /locus_tag="VFF98_14515"
     CDS             complement(10815..11615)
                     /locus_tag="VFF98_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU64895.1"
                     /translation="MAGKDEERSAEGQRKPLRDVDRMGGFAKGLSVIEAFGEGRNRLT
                     IAEVARLANLDRASARRCLLTLVEKGYAFTDDRYFELTPRILRLGHSYLAASLPRLIR
                     PSLDWLADEIRESCSAAVLDGTEVVYIARASHHRIVGAGLHPGSRLPAYCTSLGRVLL
                     ANLPEEQARDVLESSDRQRFTDNTLTTVKALMAELKKVRAQGYAYIDQELEQSSRSIA
                     VPVRNFSGETVAAVTVGLHVSRGTPERIMGEILTAMLSMQAQLAEVLP"
     gene            complement(11670..13295)
                     /locus_tag="VFF98_14520"
     CDS             complement(11670..13295)
                     /locus_tag="VFF98_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU64896.1"
                     /translation="MADYDYIIVGAGSAGCVLANRLSADPAIRVLLLEAGGSDAHPFI
                     TMPRGLAKVMANARYVWPFATRPEPRSNGVAEIWARGRVLGGSSAVNGMVYVRGARAD
                     FDALAEQTSPDWNWDHMARAYRAIEAHELGEGPARGGSGPLPVTLPETRPALSEAVIA
                     AGQALGLQRCEDVNDPADSARIGYAPRTIWRGRRQSAAAAFLRPARKRPNLTVITGAV
                     VDRLLFEGQRATGVAARIRGAEVSFHARREIVLSAGTHASPAILQRSGVGPAAVLAGL
                     GIPLVADRPGVGENLFEHRGLVFQWRVADRLSQNRALRGGGLLGSVLRYYLTRRGAMA
                     GGAYDMAAYVRSDPALERPDLQLLIAPYSLDFNAVPLQIEGHGGFNICVYPIRPLSRG
                     RVVIDSRDPAVLPRIEPHYGSHPQDTALLPVMAEVVRRLVAQPPLAALVEAETRPGPA
                     YRTPAELDEAWLRFGYANYHSAGTCRMGKDAAAVVDPACRVRGTAGLRVVDTSLFPFM
                     LAGNTNAPAMALAWRAADIILSSCGMDEAAGKR"
     gene            complement(13295..14800)
                     /locus_tag="VFF98_14525"
     CDS             complement(13295..14800)
                     /locus_tag="VFF98_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU64897.1"
                     /translation="MTTLTSDPRVAPLAHPDKLYIGGAWVAAGSGRRLRVINPATESL
                     YVEVAEADEADIARAVGAARAAFDEGPWPRLSPAERGAWLQRLADGIDARAEDFAAIW
                     PNEMGIVHAMAKAMAGGIAYTYRSYARIAERFAFVEERPTVSGAKLGLLVREPVGVVG
                     AIVPWNAPAALAALKVAPALAAGCTLVLKASPEAPGHALLLAEVADSIGLPAGVLNVL
                     TADRQASEALVRDERVDKISFTGSSAAGCHIASILGARMARYTMELGGKSAAIILDDY
                     DVEAAATALAKRAPDMTGQVCAALTRVIVPRARHDRFVEALGAALARVTVGDPFDPAT
                     RMGPLATARQRDLVEGFIAGARASGATLACGGGRPAHLPRGFYVEPTVFAHVDPADRI
                     AREEVFGPVLSVIAADSEAEAVRIANASAYGLNNAVFTDDIDRAYRVARALRSGTVGH
                     NGQRMDFSIAFGGFKRSGVGREGGVEGLLPYLESKTVLLQERPSALPPVQG"
     gene            complement(14830..>15304)
                     /locus_tag="VFF98_14530"
     CDS             complement(14830..>15304)
                     /locus_tag="VFF98_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU64898.1"
                     /translation="PAAALRALTKPVVAAIDGPCITGALEMALSCDIAIATPAARFAD
                     THCQLGLFPRWGGGTLLPSAIGVWRARQMMLTGAPIDAGTALAWGLVNELVPAPGLLA
                     RAAELAQAMAQLAGARPQAFALHAELLDTLALPPPARALEADLLARFDLARFDLA"
BASE COUNT         2364 a         5050 c         5431 g         2459 t
ORIGIN      
        1 ccccgccgca gccggccgcg cggggcagga tcagcgccgg ccatggcgct tacaagtcgg
       61 gccagtcggc cagcgggtca gtctcgatga tcacgctggg cttgctctgg cgcacctggc
      121 tgcgcggcgg cacggagcgc gtgagccaga cattgccgcc gatcacgctg cccgcgccaa
      181 tggtgatgcg gcccagcacg gtggcgccgg catagatcgt cacgtcatcc tcgatgatcg
      241 ggtggcgcgg ggtgttgagc aggtggccgt cctcgtccac cgtgaagctg cgcgcgccca
      301 gcgtgaccgc ctgatacagc cgcacccgcc ggccgatgat cgccgtctgg ccgatgacga
      361 cgccggtgcc atggtcgatg aagaagctct cgccgatggt ggcgccgggg tggatgtcta
      421 tgccggtgtg cgaatgggcg atctcggcga tgatgcgggc caccagcggc gcgcccagct
      481 tgtagatctc gtgggcgatg cggtggtgga tcagcgcggt gaaggaggga tagcacagca
      541 gcacctcatc cacgctgcgg gcggccggat cgccggcaaa ggcggcttcc acatcgctgt
      601 cgagcaggcg gcggatatcg ggcaggcggg cggccagctt gcgaaccatg cgggtggcgc
      661 cggcatcggc ggcagtggcg gcctcggccg ggcccagggc ggccagctcc atgcgcagct
      721 gggcggccag cgccggcagc gccgagccga gcgaggcctc gacaaaggca tcctcattgg
      781 ccagcgtcag ctctggcggg cccagccgca tgggaaacag cgccgcggcc agcgtgtcga
      841 caatgcggcc cagcgcgtgg cgggcgggaa agccatgggc gccataggcc ggatcacgca
      901 gatgttcccg ctcacgcgtg gcgcgccagg ccgcgcggcg ctggcgcagg gccgcggcga
      961 tatcggccag atcgatgccg gatgcagaag gggcggccgc ctcttcgctg gtggcagggc
     1021 gcgatccggg ggatggcacg gcggctggtt cctctttgca caggtgatga ttgcacaggc
     1081 gatggctggc ccggatggcc ggctgtggtt tgctgtaaaa cgcctatgtg aatggtaggg
     1141 tattgtcgcg caatggctgt ttgccggcgg ccaagaaaat cttggcgatg cccggcaagg
     1201 caggctacat gcttgcggca aagggacaaa cgccgccgcc cgggccccgc cgcccggccg
     1261 ctgcggcccc cctgcgagga caggacgatg gcagtgctga gctgggtgat gatgatggcc
     1321 atgccagtgg ggccggtggg gcctgtgctg ccggccgtgg tggagccgcc ggccgcgctg
     1381 gcgcagccgc tggccgccgg tgcgccggcc ggtgctgggc cagagggggc cagcccgccg
     1441 cccactaccc cgccgcccgc cgcccagccc gatgcggccg cccaggcaga tgcccccgtc
     1501 cccgcatccg ccccgccgcc cgccagcgac gacatgttgg ccaccccgcg tgacccgtgg
     1561 cagaaaacca accgcaccct ctttgccatc aacaatgatg tcgatcatgt gctgatcgcc
     1621 ccggtggccc atgcctatgt gtttgtcttt ccgcgctttg tgcggcatcg catcggcaat
     1681 gtcgtgggca atatgaacga gccgatggtg gccatcaaca atctgctgca gggccatccg
     1741 ggccgtttcg tgcacacgct cagccgcttt gcgctgaaca gcacgatcgg ccttggcggc
     1801 atgttcgatg tggtggcgtg gaaactgccg caccgcgatg ccgatttcgg ccagacgctg
     1861 ggccgctggg gggcaaagcc gggcccctat gtggtgctgc cgttcttcgg cccgtcggac
     1921 ctgcgtgacg ggtttggccg ggcggtggat attgccgctg atccggtggg ctgggtcttt
     1981 ggcgatttcc tcaccacttt cggggcctcc aggctggggg cggaaatcgt gcatggccgg
     2041 gccgaggcgg agccggcgat cagggccctg cgcgattcca ccgatcctta tgccacggcg
     2101 cgttcgggct atattcaggc gcgcgcggcc atcttgcgag acaccaccgg caaggccgag
     2161 gccctgccgg atttcgacgc gccctgaggc cgcataacct ttgtggcggg gcctttgcgg
     2221 cgggcccttt gcagcggtcc cttgcgcggc ccaccccatg gatgaaaccg gagcgataat
     2281 catgacacga gtgctgatgg cccttcttcg ccgggtcggc gcgccgctgg cgctgggcat
     2341 gggcgcggcc gcggccttgg tgccggccgc tgccatggcc caggcgcgcg atgcgcaggc
     2401 cgaggcctat gtcgatgcca atgcgcgccg cgtgctgggg gtgctcaacg atcatcacct
     2461 gtcggtcgag gggcgccgcc aggcgttcca cacgctgatc ggcgagctgg tggactggcc
     2521 gcgcctcacc cgcttcgtgc tgggcaaata tgcccgcacc gccacgccag accagttcca
     2581 gcgctttgcc gcggcctatc gcacctatga ggaaggcgtc tatcagcgcc gcatcgacga
     2641 ctacaagggc gagcgcgccg tggtcagcgg ctcgctcatc cgcaagcccg gcgatgtcat
     2701 cgtcaccacg ctgctgtcgg gcgggcaaag cccccagccc acggcgctgt cgtggcgggt
     2761 gctgggcggc ggcgcgggct ggaaggtggt cgatgtggaa gtgaagggcg tgtggctggc
     2821 gatcacccag cagcaggatt tcgtctcgac gatcgacaat gctggcggca agatcgacgt
     2881 gctgaccgcc cagctggccc gcaacgaggc gccccggcgc tgaggccggc gcactgccat
     2941 gcttgacagg ccgcccgccc agggggcagg gcctgcgtga ttgacaaaag gggctaagat
     3001 gcgcgcgcat tggggtgaaa ccgcaatggg gttcgtggtg ctggcgctgg ccggcctgtt
     3061 cctcgtctat tcgctggggg tggcccatgc cgggcgcagc ggccagggct atgagctgac
     3121 cgctcgggtg ggcgacagcg ggggcctggc ccccggcgcc aaggtgacga tcggcggcgt
     3181 gaagatcggc tctgtcaccg ccgtcacgct cgatcccaag agctatctcg cggtgatcca
     3241 cctcagcgtt gacagcaccg tgccgctgcc caccgactcc accgccaaga tcactaccga
     3301 tggtctgctg ggcggcgccc atgtggcgct gacgccgggc ggcgcgcaag gcaatctgaa
     3361 agccggcgac gagatcgcca acacccaggg cgcggtggat ctgatcgggc tgatcggcca
     3421 gttcatccgc ccgcacgatg atgccccggc ggccggcgcc gcttctgctc ctgcggcagc
     3481 ggcaaatgcg gcagcagcaa agcccgcccc ctatccggcc ggggtggatt gagatgaccg
     3541 gcagcgatgt gccggaaagc ccgccgcccc gcctgctggc gccggtggcc gcgctgggcc
     3601 gggccacgct ggcgggcgcc ggcagcatcg gccaggtggc gctgtttgcg ctggcggcgc
     3661 tgcgcgcggc gctcaccccg cgctggtttg gcgggcagat cggccgccag ctgtggcagg
     3721 tgggctttct ctccctgccg gtggtgggga tgacgatctg gttcacaggc gccgcccttg
     3781 ccctcaacgt gtggacaggc ggcagccggt tcaacgccga acaggcgatg ccgcagatcg
     3841 ttgccatcgg catcacgcgg gagctgggcc cggtgctggc ggccttgatg ctggcgggcc
     3901 gggtggcggc cgccatggcc tcggaaattg gcgccatgcg cgccaccgag cagatcgacg
     3961 cgatgaccac cctgtccacc gatccgatgc gctatctgat cgcgccgcgg ctgctggcgg
     4021 gggttttgtc gatgccgctg ctcacgctgg tggccaattg ctttggcatc gccggcgggc
     4081 tggtggtggc ggtgcaggtg ctggatatcc cgccgcccac ctatatccag aacacgctgg
     4141 attacctgca ggactgggat gtggaatcgg gcctcgtcaa ggcggcggtg ttcggcttca
     4201 tcgtggcgct gatgggctgc taccatggca tgaacgcgcg cggcggcgcg cgcggcgtgg
     4261 gccgggcggc cacccaggcg gtggtctcct cggcgatcct catcctcgcg gcagattatc
     4321 tgctcacctc gcttttctcc cggatgtgag cctgccgatg cctgtggaaa cgcctttgcc
     4381 caagctggcc tggaaaaacg tgtcgaagcg cttcgacggg ccgccggtgc ttgcgggcct
     4441 tgatctggcc gttgctgccg ggcgcagcct ggtgatcatc ggcggctcgg gccagggcaa
     4501 gtcggtggcg atcaaggtgg cgctggggct gatcccggtg gatggcggcg aggtgctgct
     4561 cgatggcgtg aacatcgccg ggcttgatgc cgccgggcgg cgccaggcgc tggcccgggt
     4621 gggcatgctg ttccagggcg gggcgctgtt cgacagcctc agcgtgtggg aaaatgtcgc
     4681 cttccgcctc atcaatgccg agggggtgcc ccgccgcgag gcgcgcgccc gcgcgatcga
     4741 cacgctggcg ctggtgaggc tggggccgca gacggccgat ctctacccgt ccgaactgtc
     4801 ggggggcatg cagaagcggg tggggctggc ccgcgccatc atcggcgcgc ccgagatcct
     4861 gttcttcgac gagccgacca ccgggctcga tccgatcacc gcggccgcga tcaatgcgct
     4921 gatcgtcgac cgggtgcgct cgctgggctg cacggcggtg tcgatcaccc atgatctggc
     4981 ctctgcccgc acgattgccg acgagatcgc catgctgcac catggccgca tcgtctggcg
     5041 cgggctggcc gcccagctgg acagcaccga caatccctat gtccgccagt ttgtcgacgg
     5101 gcgcaccgac gggccgatca acgcggatcg ctaaggctgc tggcaaaggc gcgcgccggc
     5161 gctatagggg cgagcattcg gcaatgcaca ggatggcccc atggcgcgca aacattccag
     5221 acgtgatccc tatggcgatc ttgatcgcga ggaggaagcg ccggtcgagg tggtgccgtg
     5281 ctggctgtgc ggccggccca ccgggcagac catcgtgtgg caccaccccg tgcccaaaag
     5341 ccggggcggg cgcgatgtgg tgcccatgca ccccatctgc cagcagacgc tgatcgccaa
     5401 tttcaccaat tccgaactgc agcgccatgg catggatgtg gaagccctgc tggcccatcc
     5461 ggccgtgcgc aaattcgtcg actgggtggc caacaaggac cccgatttca ccgccaccac
     5521 cgccaagaag cagcgctgag gcagcgttgc gcttttacag ctgcgcgcgg gcagggggcg
     5581 ggctggccat tttccggaat tgatcgatca tttcggcgat catcgcgcgg atggtgtggc
     5641 gcagaagcgc ctcatggtcg aaatgcgtgt cggcgatgcc gccgttgaat tgtgacgcct
     5701 tgatcgagat cagcgccgag ccgatcaccg cattgtcaag cgcgatcagg aattcgtggc
     5761 gcagggcggc gggcaggtgg atgtctgcca gcttctgatc ggatagcgca taggtcatgg
     5821 tgggcggccg gatgccgtta agcagcagcg gcacgaggaa cggcttggcg accagcaggc
     5881 ggcgataatg cacgccattg ccggcgatcc attgcgccca gtcgtcggtg gggggcagga
     5941 tctgcacatc gcgcagggcc ttgcgcacaa ccgccagcaa gatctcgtcc ttattggcga
     6001 aatgatggta gagcgaggcg gaattgacct gcatgcgcgc ggccagatgg cgcaggctca
     6061 aggcgtccag cccttcgctc tcgagaatct gcgtggccgc cgtgatcacg agatcgagcg
     6121 atatcagcgg cgattttggt ctggccacgt tgctggcgcc ctcctgcccg gtgtgatggc
     6181 tgtgtttcaa tgcgcatcgg ggctgggggg tcaagcggat tgtcgcggcg ccggctcttt
     6241 ggcgatcagc cgcaccgggg cgccatgcgc ccttgacagc aataaccaaa cgatgtttga
     6301 taaatgcgtc cgcttcgggg caacgggctg cctgtccttg ccagcgcacc gggcccacag
     6361 cccaattcgg gagagcttgc atggctggca tggataccaa cgtcgccctc gacacgtcgg
     6421 tagtggcgca attgatcgag catattcgca acaagagcac ggacagggcc gaacaggaac
     6481 tgcgcgtgcc ggccagccag ttcacctgcc ccgaccgcgc ccgcgcggaa atcgccctgc
     6541 tcaaaacctg cccgctggtg attggccatg cttccgaagt gccgcaagcc ggtgatttca
     6601 tcacccgcga tctgttgggc gcatcggtga ttgtcagccg gggcaaggat ggccgcattg
     6661 ccaccttccg caacatgtgc ccgcatcgcg gcggccgggt ggaacagaag gaggcaggcc
     6721 gcaagcacat cttcatgtgc cagtatcacg gctggtcgtt caatgccgag gacggggcgc
     6781 tgcgcccggt gccctacgag gacagtttcg gcccgatcga ctatgcctgt tcgggcctgc
     6841 agcggatccg caccgaggtg cgccacgggc tgattttcgt cgacctgtcg gacaactgcg
     6901 agcgcgatat cgccagcttc ctgggcgcgg aagtggaggc ccagctggcg ccctggcagc
     6961 tcgatcagtc gcgggtctat ctcgacaaga gcttcaccgt gccgatcaac tggaagctgg
     7021 tgatggatgg cgcggtggat agcctgcatg cgcaatacct gcatcccggc cccggcaatg
     7081 tgggcgcacg cacgctgacg aatattgcgg tgtttcgcgc ctttgggccg cacgggcggc
     7141 tgtttgtgcc gcgcacgcgg atgaagaagc tggtggatca gggcgaggag gccgctgcta
     7201 ccaccaacta tgtggcctcg atcatgctgc tttatcccaa tgcgctgatg gccgccgcgc
     7261 ccgatcatgt cgagttctgg acggtgtggc cgtcggccaa tccggcggaa tgcacggtgc
     7321 gcatccgctt cttcgtgcgg cccgagattc tctcgccgga gatggaggag cgcatcgaca
     7381 ggagctgggc cattctcgaa aatgccgcca caaccgagga ttggccgatg gaggtgtgga
     7441 ttcaggaaaa cgccatggcc tggccagaca cggcctttcg ctatggtcgc aacgaggttt
     7501 cggcccagca cctgcatcat gagctgatcc gggcgctggc cgccagctga gctttcgacc
     7561 ctgaccccca gccccacgcg gccggtgtgc agcttgacaa gctgggcacc gctggattat
     7621 gtgcgaatat ataactatag agcgaatatc gcactaaatt ggacgagggg cacatggccg
     7681 gaccggatca gtcaggcagc gggcaatcgc tggaaagttt cgacattcac gcgcccgatg
     7741 cgcgggtggg ggcggaatgc ctgtgggcgc agatgcgcgc gcagcccggc ctttaccaca
     7801 gcgatcgcta tggtggtttc catgtggcgg cgcgcttcga ggatgtgatg gcggtgctga
     7861 tgaagccggc gctgttctcc tcggccaggg gcatcacctt gccgccgccc accgcggtgc
     7921 gcagcctgca tatcccggcc gaggtcgatc cgccgctgca tggcgaatat cgcgcgctgc
     7981 tgatgccttt cgtcaccccc gaacaggccc gcttgcgtga gccggcgatc cgtgcgctgg
     8041 tgcgccagtt gctggcacgc tttcccgatg ggcagccggt ggatttcgtg cggctgctgg
     8101 gccggcccct gccgatcctt gtcgcgctgg atctgctggg catgcccggc gaggatgccg
     8161 aggcgctgga gacgctggtg ggcgagatcc accgcgatgt ggcggccggc gtttccagcg
     8221 gcgcgatcga ccgtttgcag gcctatgccg aacgggtgct ggagcgccgc cgcgccatgg
     8281 ccagcgatcc gggcgaggat ctggtgtcct cggtgttgct gggccaggtg cagggccgcc
     8341 cgcttaccgg cgaggagcag atgtcgatca tccgcctgct gttgatcggc ggcttcgaca
     8401 ccacttcggc cacgctgtcg gagatgatgc gctggctggc cgagacgccc gaggcggccg
     8461 cccgcctgcg cgccgatccg gcgctgatcc cggcggcggt ggaagagatg gtgcgggtgg
     8521 cctcgccttc cacctatctg cgccgcgaag tgacgcagga ttgcgaattg ggaggcacgc
     8581 ccttgaagga gggggattcg gtgctggtgg cctttggcgc ggccaatcgc gatcccgcca
     8641 aattcgcctg cccggaaacg gtggatccgg cgcgcaagcc caacccgcat gtggggtttg
     8701 gcgcgggcca tcaccgctgc atcggctcgt tcatcgccaa ggtggagttg aaagtggcct
     8761 tcgaggagat cctggcgcat tttgccgatt tccgcctcga tggccctgtc cgctacacca
     8821 gcgggctggg gcaggggctg atctcgctgc cgatggtgtt tgcccgcgcg gcccgctgac
     8881 cggcccgcag ttcaggaaag gatgcaaagt gcccgcgata gagccaggcc gctatgaatg
     8941 gatcaaggtt gccagggggg aggacggcat cgccacgctg acgctgaaca acccgcgccg
     9001 caagaatgcc ctggcccgcc cgatgcaccg cgaactggag cgcatctggg acgatatcga
     9061 tgccgacgac gatatccggg tgacgatcct gaccggcgag ggcgatgcct tctgcgccgg
     9121 cacggatctg tcctttcagg atggcgagaa cagccagggc cgcaaggggc gcccgcccac
     9181 gcgcggcgcg cgccggctgt tctggaacat gctcgacagc gagaagccga tcatcgccaa
     9241 ggtgcgcggg ccggcctatg gggtgggcgt gaacattgct ctcgcctgcg atatggtgat
     9301 cgccgccgag ggcgcgcgct tctgcgattc ccacgtgcgc atggggatcg cgccgggcga
     9361 tggcggggtg gcttttttcc cgctgctgct gggttttcac cgggccaagg aatatctcat
     9421 gctgggagag ccgatcgagg cgcgcaaggc ggccgagatc gggctgatca actattgcgt
     9481 ggccgatgcg gaacttgacg cgctggtgga ttcctatgcg cagcgcctgg ccaatggggc
     9541 gccgctggcg attgcctatg gcaagatgtc ggtcaacctg ctgctcaagc agatgaccgc
     9601 cggcgcgttc gagacatcgc tcgcctacga ccagctgacc ctgttcaccg aggaccacaa
     9661 ggaaggcgcg cgcgctttcc tcgagaagcg cagcccgcgt ttcaccggca gctgagcgcc
     9721 tgaaggcccc ccgggcgcag tgcctgcgcc ggggggatgc gccttcagtc gatcggcgga
     9781 ttgcgctcgg gatggaacac gcgctgggcc gtgcgcggat accacggcgc gatggcgctg
     9841 gccgcctcca gggctgtgcg ttctgctgcg gcaagggtgg tggtggctgc ggcaagattt
     9901 tcgcgcagct ggctttcgga acgggcgcca acgatcaccg cgctgatgcc gggccgcccg
     9961 atcagccagg ccagcgccag ttgcgaaggc gtgcgcccgg gccctgcgct ggcggccagg
    10021 cgcgccagtt cggccacgat gccgcgcgca tggggcgtgt ctgttccggc aaactggcgg
    10081 gtggccagca gccgcccttc gctgccggca tcggcaaatt tgccgctgag gtagccttgg
    10141 gccagcgggc tgtagaccag cgtgccgacc ccatgatcga gcgctgcggg cagcagttcc
    10201 tgttcggcct gccgataggc cagcgaatag aggatctgct ggctgacata gctttccagc
    10261 ccatggcggg cagacaggcc gatggccttg gccagctgcc aggcaaaatg gttggagcag
    10321 ccgatatggc gcaccttgcc cgcgcgcacg agatcgtcga gcgcgcgcag cgtttcctcc
    10381 atcggcacca gcccgtcgtg gttgtgcacc tggtagaggt cgatccagtc ggtgccgagg
    10441 cgccgcaggc tggcctcgca ggcgctcacc agatgccggc gcgacaggcc gatgtcatgc
    10501 gtgctgggcc ctgtgcggcc gaacgccttg gtggcgatga cgacatcgcg ccgccgggtg
    10561 ccgagcgctg cgcccagcag gctttcggca tggccgtcgg cgtaattgtc cgcggtgtcg
    10621 aacagggtga cgccatgatc gagggcgagg gccacctggc gcttggcctg cgccagatcg
    10681 gtagtgccga tggcggcgaa cttgccgccg ctctcaccgc caccgaaagt catcgtgcca
    10741 aagccgatga ccgacacctg caggccgctg cggccgagaa agcgctgctc catgccgccc
    10801 tcccgcgcct ggccctatgg cagcacttcg gccagctgcg cctgcatgga cagcatggcg
    10861 gtgaggatct cgcccatgat ccgctccggc gtcccgcgtg agacatgcag gcccacggtc
    10921 acggccgcca ctgtctcgcc cgagaaattg cgcaccggca ccgcgatcga gcgcgagctt
    10981 tgttccagct cctggtcgat ataggcatag ccctgcgcgc gcactttctt cagctcggcc
    11041 atcagcgcct tcaccgtggt cagcgtattg tcggtgaagc gctggcggtc gctgctttcc
    11101 agcacgtcgc gggcctgttc ctcgggcagg ttggccagca gcacccggcc cagcgaggtg
    11161 caataggccg gcagccggct gcccggatgc agcccggcgc cgacgatgcg atgatgcgag
    11221 gcgcgcgcga tatagaccac ttcggtgcca tcgagcacgg ccgccgagca gctttcgcgg
    11281 atctcgtcgg ccagccagtc gagcgagggg cggatgaggc gcggcagcga ggcggccaga
    11341 taggaatggc ccaggcgcag gatgcgcggg gtcagctcga aatagcgatc atcggtgaag
    11401 gcatagccct tttccaccag cgtcagcagg cagcgccggg cagaggcccg atcgagattg
    11461 gccagccgcg ccacttcggc aatggtcagc cggttgcgcc cctcgccaaa ggcctcgatc
    11521 accgacaggc ccttggcaaa cccgcccatc cgatcgacat cgcgcagcgg cttgcgctgg
    11581 ccttctgcgc tgcgctcttc atccttgcct gccacgtttc cggccactgc gttcccttct
    11641 ttctgtgcga ttattgcacc atgtgtgctc tagcgctttc ccgccgcttc gtccatgccg
    11701 caagaagaca ggatgatgtc ggccgcgcgc caggccagcg ccatggccgg ggcgttggtg
    11761 ttgccggcca gcatgaaggg aaagagcgag gtatccacca ccctcagccc ggcggtgccg
    11821 cgcacccggc aggccggatc caccacggcg gcggcgtcct tgcccatgcg gcacgtgccg
    11881 gcggaatggt agttggcata gccgaagcgc agccatgcct catccagctc ggccggggtg
    11941 cgataggccg ggccgggccg cgtttctgcc tccaccagcg cggccagcgg gggctgcgcc
    12001 accagccggc gcaccacctc ggccatcacc ggcagcagcg cggtgtcttg cgggtggctg
    12061 ccgtaatgcg gctcgatgcg gggcagcacg gcaggatccc ggctgtcgat caccacccgc
    12121 ccgcgcgata gcgggcggat cgggtagacg cagatgttaa agccgccatg gccctcgatc
    12181 tgcagcggca ccgcgttgaa atcgagggaa tagggggcaa tcagcaattg caggtcgggc
    12241 cgttcgaggg cggggtcgga tcggacatag gcggccatgt cataggcgcc gccggccatc
    12301 gccccgcgcc gggtgagata atagcgcagc acgctgccca gcaggccgcc cccgcgcaag
    12361 gcgcggttct gcgacaggcg atcggccacg cgccactgga acaccagccc gcggtgctcg
    12421 aacaggtttt cgccaacgcc gggccggtcg gccaccagcg gtatgcccag gcctgccagc
    12481 accgcggccg gccccacgcc cgaacgctgg aggatggccg ggctggcatg ggtgccggcg
    12541 ctaagcacga tctcgcgccg ggcgtggaag gacacttcgg cgccccttat ccgcgcggca
    12601 acgcctgtgg cccgctggcc ttcgaacagg aggcgatcga ccaccgcccc ggttatcacc
    12661 gtgagattgg ggcgcttgcg ggccgggcgc aggaaggccg ccgccgcgct ttgccgccgg
    12721 ccgcgccaga tggtgcgcgg ggcatagccg atgcgggcgc tgtcggccgg atcgttcaca
    12781 tcctcgcagc gctgcagccc cagcgcctgg ccggcggcga tcaccgcctc gctcaaggcc
    12841 gggcgggttt cgggcagggt gacgggcaac gggccagagc cgccccgggc cgggccttcg
    12901 cccagttcat gcgcttcgat ggcgcgatag gcgcgggcca tgtggtccca gttccagtcg
    12961 gggctggtct gctcggccag cgcgtcgaaa tcggccctgg cgccgcgcac atagaccatg
    13021 ccgttgaccg cgctggagcc ccccagcacc cgtccgcgcg cccagatttc cgccacgccg
    13081 ttcgagcgcg gttccggccg tgtggcaaag ggccagacat agcgggcatt ggccatcacc
    13141 ttggccaggc cgcgcggcat ggtgatgaac ggatgggcat cgctgccgcc ggcctccagc
    13201 agcagcacgc ggatggcagg atcggcgctc aggcggttgg ccagcacgca gccggcagag
    13261 cctgcgccga cgatgatata atcgtaatcg gccatcagcc ctgcaccggc ggcagcgccg
    13321 aaggccgctc ctgcagcagc acggtcttgc tttccagata gggcagcagg ccttccaccc
    13381 cgccctcgcg ccccacgcca gagcgcttga agccgccaaa ggcgatggag aaatccatgc
    13441 gctggccatt gtgccccacc gtgcccgagc gcagcgcgcg cgccacgcga taggcgcggt
    13501 cgatatcatc ggtgaaaacg gcgttgttga gcccataggc cgaggcattg gcgatgcgca
    13561 ccgcttcggc ctcgctgtct gcggcgatga ccgacagcac cgggccgaac acttcttccc
    13621 gggcgatgcg gtcggccgga tcgacatggg caaaaacggt gggctccaca tagaagccgc
    13681 gcggcagatg ggccggccgg cctccgccgc aggccagcgt tgcacccgag gcacgggcgc
    13741 cggcaatgaa gccttcgacc agatcgcgct gacgggcggt ggccagcggc cccatccgcg
    13801 tggccggatc gaagggatcg cccaccgtga cccgggcgag ggcggcgccg agcgcctcca
    13861 cgaagcgatc atgacgggcg cgcggcacga tcacccgcgt cagcgcggcg cagacctgcc
    13921 cggtcatgtc gggcgcgcgc ttggcgagcg cggtggcggc cgcttccacg tcatagtcat
    13981 cgaggatgat ggcggccgat ttgccgccca gttccatcgt gtagcgggcc atgcgcgcgc
    14041 ccaggatcga ggcgatatgg cagcccgcgg cgctggatcc ggtgaagctg atcttgtcca
    14101 cccgttcgtc gcgcaccagc gcttcggagg cctggcgatc ggcggtgagg acattgagca
    14161 cgccggccgg caggccgatg ctgtcggcca cttcggccag cagcagggca tggccgggcg
    14221 cctcgggcga ggccttgagc accagcgtgc agcccgccgc cagcgccggc gccactttca
    14281 gcgcggcgag cgcggccggc gcgttccatg gcacgatcgc gccgaccacg ccgaccggct
    14341 cgcgcaccag caggcccagc ttcgcgccgg aaacggtggg gcgctcctcg acgaaggcga
    14401 agcgctcggc gatccgggcg tagctgcgat aggtgtaggc gatgccgccg gccatcgcct
    14461 tggccatggc gtggacgatg cccatctcat tgggccagat ggcggcgaaa tcctctgccc
    14521 ttgcatcgat gccatcggcc aggcgctgta gccaggcgcc gcgctcggcc ggcgagaggc
    14581 gcggccaggg gccttcgtcg aaagcggcgc gggcggcgcc gacagcccgg gcgatatccg
    14641 cctcatccgc ctcggccact tcgacataga ggctttccgt ggccggattg atcacccgca
    14701 ggcgccggcc gctgccggca gccacccagg ccccgccgat atagagcttg tccggatggg
    14761 ccagcggggc aacgcgcgga tcggaggtga gggtcgtcat gagggcacct tgtgcaggaa
    14821 agggggaggt caggcgaggt cgaaacgggc caggtcgaag cgggcgagca ggtctgcctc
    14881 cagcgcgcgg gccggcggcg gcagggccag cgtgtcgagc agttcggcat gcagggcaaa
    14941 ggcctggggc cgcgcgccgg ccagctgcgc catggcctgc gccagttcgg cggcgcgggc
    15001 cagcagcccc ggggccggca ccagttcgtt gaccagcccc caggccagcg ccgtgccggc
    15061 atcgatcggc gcgccggtca gcatcatctg gcgcgcccgc cacacgccga tcgccgatgg
    15121 cagcagcgtg ccgccgcccc agcgcggaaa caggcccagc tggcagtggg tgtcggcaaa
    15181 gcgggctgcg ggggtggcga tggcgatatc gcaggaaagc gccatttcca gcgcgccggt
    15241 gatgcagggc ccgtcgatcg cggccaccac cggcttggtc agcgcccgca gcgcggcggc
    15301 cgga
//
LOCUS       DASRYE010000283         5406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_17432,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000283 DASRYE010000000
VERSION     DASRYE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17432"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1041)
                     /locus_tag="VFF98_14535"
     CDS             complement(58..1041)
                     /locus_tag="VFF98_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZU64899.1"
                     /translation="MARDTDQLARALRGWLPRGSAITGVKTLSAGHSNETYLIEGLGE
                     ILRLPPSEEGLLPPYDMARQHRVLSAVAAAAPGVPLPPVLELCEDASVLGDPFFLMGC
                     IPGEAFEYAVPAWLAADPVAGADSVCRQWFDAILALHTMPAAAMPPGGRSVGAEAQHW
                     LDVARGAEAMPALIAVLEDLARRPPRESGPPTPVHGDPKHGNCLWHEGRLTGLLDWEM
                     AQLSEPMLDLGYILMFHDQGEASLAAAGFELPGWWSPERMIAEWEQGTGRTAHEAGRY
                     AVLGQAKVAAIISVGAHLSASGRIADPRFRAFAAVLPGYVRLLEARAMVAG"
     gene            complement(1046..2254)
                     /locus_tag="VFF98_14540"
     CDS             complement(1046..2254)
                     /locus_tag="VFF98_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64900.1"
                     /translation="MTAILPNSPTRFVRNAMEDIGMITALDEMLNHQIVDTFASTATA
                     DHSWTEKIWTAIARKDGTLAIDFGLGRYHNRGILDGWAAVSRGGQQWTVRANRELRDD
                     PTETRVGPLTYEIIEPLHKVRYALAPNPHQPIAFDVTFTAEMQPFFEDRHRQREKDGF
                     RIGSDVVRYHQIGVPSGWVEIEGERITIDPADWTEYRDHSWGVRLDVGAHNADIRPTA
                     DFGDARFGEGEFMLVWSPFMLVSPDGGKVAYHFYHQVKQGRVFYSSGYRNLPDGTQEK
                     VARVRPELRYDDATRRLLGGLVHFDMLQGGTHSVEVEVVGTSGVHLGPGLYLGFDGRK
                     HGMWQGPLTVEGEAFADTLDRETLKRIRQLRDCPIRVREGDAVGYGIMETIIHGAHPE
                     LGLTAANTLI"
     gene            2609..4942
                     /locus_tag="VFF98_14545"
     CDS             2609..4942
                     /locus_tag="VFF98_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64901.1"
                     /translation="MAKSITWALAGVALGGLAMAESARADEAPHSEPSAAEIIVTANK
                     RQQSINTVPLSITAASGDTLMKRGINSTADLARIVPGLTAQPSPFNTPVYTLRGVGFY
                     ESTLSASPTVAVYTDEVPLPFSAMTKAASLDLERVEVLKGPQGTLFGNNTTGGAINYI
                     AAKPTDTFKAGVNGSFGRFDTVDLQGFVSGPLTDNLKGRVAARIVESGPWQYSATRAD
                     TLGATRELQGRILLEWTPSPRLKITFNLNGWIDKSDTQAPQRIATYISVPGNPPAAAT
                     LAYPYPPQNARAADWSTNIGALRNDDYFVQGALRADYTINPAMTLTSITAYERYKENS
                     TSDFDGTARNIADNYVRGYIDTVSQELRLTGKLPRFNWVLGANYEYDWTYDRLYYNFS
                     DSTTSWVSTDHMTFTSNFTRQDVQTAAIFANGEYEVLPGLTLQGGLRYTDSRRKFSGC
                     TLDEPGGGVAEAFNLIESWINSGTFVTIPEGGCVTFHSSPDINGATGLYGVPYVNPIQ
                     SQLNEQNLSWRTGVNYRTANHGLLYATVSRGYKAGSFPTTSASFDSQYKPVKQESLTA
                     YEVGFKQPLLDRKVQLNGALFYYDYKDKQLRGRILDAVFGPLDALVQIPKSRVWGAEA
                     ELNARPIKGLVLDVAATYLDTKILSFTGYNNAGDLQNYAGTPFPYAPKWEIVGSADYD
                     FPVNGKLNAFLGTSLTYNSATQGSIGSIPELAIKAYALLDAQIGVRDPGDKWRFQIWG
                     RNLTNTYYWTNALQSQDVYLRFTGKPVTYGMSFSYRF"
     gene            5110..>5406
                     /locus_tag="VFF98_14550"
     CDS             5110..>5406
                     /locus_tag="VFF98_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64902.1"
                     /translation="MPVADAAPQGAAILTGPIPPIPVRQRRLGPLTRRRLGRFYLALA
                     LVYAAAVAPVVAGLAPAWQALGLGIIFPGGGFLYAGGVVGVAGALLSLVAFAWIL"
BASE COUNT          978 a         1790 c         1728 g          910 t
ORIGIN      
        1 aaggcaccgg caggtgctgc gcctgccggt gccctgacgg aattggggcg ggctggttca
       61 gccggccacc atggcgcgcg cctccagcag gcgcacatag ccgggcagca ccgcggcaaa
      121 ggccctgaag cgcggatcgg cgatgcgccc gctggccgac agatgcgccc ccaccgagat
      181 gatcgccgcc accttggcct gccccagcac ggcatagcgc ccggcctcat gagcggtgcg
      241 gccggtgccc tgttcccatt cggcgatcat ccgctcgggc gaccaccagc ccggcagctc
      301 gaaaccggcc gccgccagcg aggcctcgcc ctgatcgtgg aacatgagga tatagcccag
      361 atccagcatc ggttcggaaa gctgggccat ttcccagtcg agcaggccgg tgagccggcc
      421 ctcgtgccac aggcaattac catgcttggg atcgccatgc accggggtgg gcgggccgga
      481 ttcgcgcggg gggcgccggg ccagatcctc cagcacggca atcagcgccg gcatcgcctc
      541 ggcgccgcgg gccacatcca gccagtgctg cgcctcggcg cccacgctgc gcccgcccgg
      601 cggcatggcc gcggccggca tggtgtgcag cgccaggatc gcatcgaacc actggcggca
      661 cacgctgtct gccccggcta ccggatcggc cgccagccag gcgggcacgg catattcgaa
      721 cgcctcgccc gggatgcagc ccatgaggaa gaacggatcg cccagcacgc tggcatcctc
      781 gcacagttcc agcaccggcg gcagcggcac gccgggtgct gccgccgcca cggccgaaag
      841 cacccggtgc tggcgggcca tgtcgtaggg gggcagcagc ccttcttccg atggcggcag
      901 gcgcaggatc tcgcccagcc cctctatgag ataggtctcg ttcgagtggc cggccgagag
      961 ggttttcacc ccggtgatgg cgctgccacg cggcagccag ccgcgcaagg cgcgggccag
     1021 ttgatcggta tcgcgcgcca tcggcctaga tcagcgtgtt ggcggcagtc agccccagtt
     1081 cggggtgggc gccgtggatg atcgtttcca tgatgccata gcccaccgca tcgccctcgc
     1141 gcacgcggat ggggcaatcg cgcagctggc ggatgcgctt cagcgtctcg cggtccagcg
     1201 tgtcggcaaa ggcctcgccc tccaccgtca gcgggccctg ccacatgcca tgcttgcgcc
     1261 cgtcgaagcc cagatagagg cccggcccca gatgcacccc cgatgtgccc accacttcga
     1321 cctccacact atgcgtgccg ccctggagca tgtcgaaatg gaccaggccg cccagcaggc
     1381 gccgcgtcgc atcgtcatag cgcagttccg ggcgcacgcg ggcgaccttt tcctgcgtgc
     1441 catcgggcag gttgcgatag cccgaggaat agaacacccg gccctgcttc acctgatggt
     1501 agaagtgata ggcgaccttc ccgccatcgg gggagaccag catgaagggg ctccacacca
     1561 gcatgaactc gccctcgcca aagcgggcat cgccaaaatc ggcggtgggg cggatatcgg
     1621 cattatgcgc gcccacatcg aggcgcacgc cccagctgtg atcgcgatat tcggtccaat
     1681 ccgcagggtc gatggtgatg cgctcgccct cgatctccac ccagcccgaa ggcacgccga
     1741 tctggtgata gcgcaccaca tccgagccga tgcggaagcc atccttctcg cgctggcggt
     1801 ggcggtcctc gaagaagggc tgcatttcgg cggtgaaggt cacgtcgaaa gcgatcggct
     1861 gatgcgggtt gggggccagt gcatagcgca ccttgtgcag cggctcgatg atctcgtagg
     1921 tcagcgggcc aacgcgggtt tcggtggggt cgtcgcgcaa ttcgcggttg gcgcgcaccg
     1981 tccactgctg ccccccgcgc gagacggcgg cccagccatc gagaatgccg cgattgtgat
     2041 agcggcccag gccaaaatca atggccagcg tgccatcctt gcgggcaatg gcggtccaga
     2101 tcttttccgt ccagctatga tcggcagtgg cggtggaggc gaaagtatcg acgatctggt
     2161 gattgagcat ttcgtccagc gcggtgatca tgccgatgtc ttccatggca ttgcgcacga
     2221 agcgggtggg cgaattgggc aaaattgcgg tcatttccga ctccagtctg aacgctgaat
     2281 tggcgggcgg ccgaggcggt gggggaaggg gcttcccaca aggccttgcc ggtttatcct
     2341 gttcccgctg cctcgtataa agcgtcagac gatttcgtct acaaattttt gccgattcgg
     2401 atgggaagag aagcgccggg tgcggcgggt ggtggcagaa gcgcgcaaaa gcgcgggctg
     2461 cgcatggctt ctaaaattgc cggacgaaga tatgatatga aatcgtcgaa cgatattgac
     2521 ggtcagtgtc gatgtcggta agccgtccct cgtgaagctg gcgacgcagt gcggatagcc
     2581 ggctcgcggg gatagggaag gatgcgagat ggccaaatcg ataacatggg cgttggctgg
     2641 cgtggcgctg ggcgggctgg caatggccga aagcgcccgc gccgatgaag cgccgcacag
     2701 cgagccaagc gccgccgaaa tcatcgttac cgccaacaag cggcagcaat ccatcaacac
     2761 cgtgccgctg agcatcaccg ctgccagcgg cgacacgctg atgaagcgcg gcatcaacag
     2821 caccgccgat ctggcgcgga tcgtgccggg cctcacggcg cagccctcgc cattcaacac
     2881 gccggtttac acgctgcggg gggtgggctt ctacgaatcg acactgtccg cctcgcccac
     2941 ggtcgccgtc tacaccgatg aagtgccgct gcccttcagt gccatgacca aggccgcctc
     3001 gctcgatctg gagcgggtgg aagtgctgaa agggccgcag ggcacgctgt ttggcaacaa
     3061 caccaccggc ggcgccatca actacatcgc cgccaagccg accgacactt tcaaggccgg
     3121 cgtcaacggc agctttggcc gcttcgacac ggtggacctg caaggctttg tcagcggccc
     3181 gctgaccgac aatctgaagg gccgggtggc cgcccgcatc gtcgaaagcg ggccctggca
     3241 atatagcgcc acccgcgccg acacgctggg cgccacgcgc gaattgcagg gccgcatcct
     3301 gctggaatgg acgccaagcc cgcggctgaa gatcaccttc aacctcaacg gctggatcga
     3361 caaatcggac acccaggcgc cccaacgcat tgccacctat atttccgtgc ccggcaaccc
     3421 gccggcagcg gccacgctgg cctatcccta tccgccgcag aacgcgcggg cggcggattg
     3481 gtccaccaat atcggtgcgc tgcgcaacga tgactatttc gtgcagggcg cgctgcgggc
     3541 cgattacacc atcaacccgg cgatgacgct gacctcgatc accgcctatg agcgctacaa
     3601 ggaaaattcc acgtccgatt tcgatggcac cgcgcgcaac atcgccgaca attacgtgcg
     3661 cggctatatc gacaccgtgt cgcaggaatt gcggctgacc ggcaagctgc cccgcttcaa
     3721 ctgggtgctg ggcgccaatt acgagtatga ctggacctac gaccggctct actacaattt
     3781 ctcggattcg acgacctcat gggtgtcgac cgaccatatg accttcacct ccaacttcac
     3841 ccgccaggat gtgcagacgg ccgcgatctt tgccaatggc gaatatgaag tgctgcccgg
     3901 cctcaccctg cagggcgggc tgcgctacac cgacagccgc cgcaaatttt ccggctgcac
     3961 gctggacgag ccgggcggcg gcgtggccga agccttcaac ctcatcgaat cctggatcaa
     4021 ttcgggcact ttcgtgacga tccccgaagg cggctgcgtc accttccatt cctcgcccga
     4081 tatcaacggc gccactggcc tctatggtgt gccctatgtc aacccgatcc agagccagct
     4141 taacgagcag aacctctcct ggcgcaccgg cgtgaactac aggacggcca accatggcct
     4201 gctctatgcc actgtcagcc ggggctacaa ggcgggcagc ttccccacca catcggcctc
     4261 cttcgattcg caatacaagc cggtgaagca ggaatcgctg accgcctacg aagtgggttt
     4321 caagcagccg ctgctcgatc gcaaggtgca gttgaacggc gcgctgttct actatgacta
     4381 caaggacaag cagctgcgcg gccgcattct cgatgcggtg ttcggcccgc tggatgcgct
     4441 ggtgcagatc cccaagtcac gcgtgtgggg cgccgaggcc gaactgaatg cccggccgat
     4501 caaggggctg gtgctggatg tggcggccac ctatctcgac accaagatcc tcagtttcac
     4561 cggctacaac aatgccggcg atctgcagaa ttatgccggc acgccgttcc cctatgcgcc
     4621 caaatgggaa atcgtgggca gcgccgatta tgatttcccg gtgaacggca agcttaacgc
     4681 cttcctcggc acctcgctca cctataattc ggccacgcag ggcagcatcg gctccattcc
     4741 ggaactggcg atcaaggcct atgcgctgct cgatgcacag atcggcgtgc gcgatccggg
     4801 cgacaagtgg cgcttccaga tctggggccg caacctcacc aacacctatt actggaccaa
     4861 cgccctgcag agccaggacg tgtatctgcg cttcaccggc aagccggtga cctatgggat
     4921 gagcttcagt tatcgtttct gatcaggcgg gcggcgggcc ttgtttgccg cccattttca
     4981 tcatacgata tattcatacg aaagccgccg gcaaaaggcg gctacggaga gcgagcagcg
     5041 ccactggccc ccaacgggcc ggcaaaagcg cgggctcagg aagagcgagg caccatggct
     5101 tcccacgcat tgcctgtcgc agatgcagcg ccgcagggcg ctgccattct caccgggccc
     5161 atcccgccca tccctgtgcg gcagcggcgg ctggggccgc tcacccggcg gcggctgggg
     5221 cgtttctatc tggcgctggc gctggtttat gcggcggctg tggcgccggt ggtggcgggg
     5281 ctggcgccgg cctggcaggc gctggggctg gggatcatct tccccggcgg cggcttcctg
     5341 tatgccggcg gggtggtggg ggtggcgggc gcgctgcttt cgctggtggc ctttgcctgg
     5401 atcctg
//
LOCUS       DASRYE010000284        12002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_123672,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000284 DASRYE010000000
VERSION     DASRYE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123672"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFF98_14555"
     CDS             <1..360
                     /locus_tag="VFF98_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64903.1"
                     /translation="VRIANRRLLDIYTRHRLALIEMPRQVPRIEQVIRYTSLEMLNRG
                     NIRLYLSQHPEFDSEKTENHIFYIETIIHALMRRYVLEAPEGLPPDELVEQICELILS
                     YLGRQRIDPVAREAGRP"
     gene            357..962
                     /locus_tag="VFF98_14560"
     CDS             357..962
                     /locus_tag="VFF98_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU64904.1"
                     /translation="MTEPAGTAPRRAANGALEAQIIEAAYACFERFGITKTTMEDIAR
                     GASVSRQSVYRYFARKDDILDEICAREAVKINAIVRARIRRDGNFADTLTDALCVIIS
                     EADKNPYLRQVIDAPSVQTLASSPASRAYRLNRSYWQGMMQRAAAAGQLADDLSLDEI
                     VSWLLMMQSVLQLRLASGQIDAAQLHRLVRRFAVAPLLKSV"
     gene            1165..2328
                     /locus_tag="VFF98_14565"
     CDS             1165..2328
                     /locus_tag="VFF98_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64905.1"
                     /translation="MLKSTQAPAERLLTQAQMQQLAGLEDSNPAEVFREIDHLPTDIY
                     ASEARFELERQKLFRGRPVPVGVSALLPTAKSHVAIDDYGTSLLLTRDEEGQVHAFFN
                     VCTHRAIKLCQAREPQKKGLVVCPYHAWSFDMKGRLKALPRPEIFPGLDKSQMGLVEL
                     ECVEAGGLIWVNLDRERKADFALVRGPLGADLDAFGLGQQKICHHLRVDVKANWKLIV
                     DSFSENYHVTRLHAQSLGGMFVDRKTSCEMIGEHLRVMSGRAEFRQGEEIASYEAFRK
                     SAVAHYTIVAGALIITSPSYVSVMLLSPQAVDHTVVNYYMMVDQLPETEREIAHFDRS
                     LALMKQITTKEDFWVAELGTIGAKSGVVPFMTCGGMEREMITFHQSVDAILAG"
     gene            complement(2394..3887)
                     /locus_tag="VFF98_14570"
     CDS             complement(2394..3887)
                     /locus_tag="VFF98_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU64906.1"
                     /translation="MPIPPSGHQAVPLSHPRQIFVDGAWQDSAGDGLIDVICPASEAL
                     YFQVASAGVADVQRAIAAARTAFDEGPWPRMAPAERAAYLRAMAAELEARAADIAQIW
                     PNEMGILHSMAQAYAPGAAGFFRYYADLADSFPFEEQRKTVSGAALGLLVREPVGVVG
                     AIIPWNGPIMITAFKVAPALLAGCTVVIKGSPEAPGHALLMAEVAEKVGLPRGVLNVV
                     IADRQASEALVNSPDVDKIGFTGSSATGRHIASILGGRMARYTLELGGKSAAILLDDY
                     DLEAAARELALRECDMSGQVCASLTRIIVPRHRAERMAEAIGAQFSQVVVGDPFDPAT
                     QMGPLATRLHRDRVLGHIERARADGFRLVAGGGRPAHLERGFYVAPTVFAGVDNASAL
                     AREEVFGPVLAVIPAQDEEDAVRLANASPFGLNGAVFTNDPDRAYAVARQLRSGTVGH
                     NGHLVDFTIAFGGFKQSGVGREGGVEGLLPYLESKTILLNGRPAHLG"
     gene            4001..4744
                     /locus_tag="VFF98_14575"
     CDS             4001..4744
                     /locus_tag="VFF98_14575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU64907.1"
                     /translation="MTARRSIIVTGGASGIGEASARLAAARGWAVTIADRNPAGEDIA
                     AAIRAEGGEACFIATDVTEEASVRALVAGALAAHGALHGAINSAGIAGCSRPIHEIEL
                     ADWDRLHAINLRGMFLCLKHQVAAMWEQRAGSIVAVSSAAALKGLPWSSDYCGSKAGI
                     DGMVRGAAMDCAEQGIRINALLPGPTLTPLAVGSSNANPALAKTRIRPMERVADPIEI
                     ARAAVWLVSDEASFVTGIAMPVDGGMVIA"
     gene            complement(4783..5883)
                     /locus_tag="VFF98_14580"
     CDS             complement(4783..5883)
                     /locus_tag="VFF98_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64908.1"
                     /translation="MSEMLDTEELREAIRTVLADRSGGVIAIPDEAGRGLDRALWQEM
                     AGLGWLGLALPEEEGGLGLGLEHLAVLYEELGRELASVPVLPAMLAAGALVRHGGAEA
                     RARWLARLADGSCLAAIALPEAAALPAAITLPAALPQIAADGRIAGAVDHVPFADVAD
                     LLLLPVRQADGALALALVPKGAAGLAITPRPLIDLTRSSARIVLEGAVAADVLAVDEE
                     GWAALLDHAAMGLACDCVGAAEALLERTVDYLGMREQFGRPIGSFQALKHRMADWKVR
                     IEAVKVLARHSAALLQAGSAEASAIASGAKAYAGDVFAAFSGDAVQLHGGIGFTWEHP
                     CHLFLKRAKLSQQLFGSSATHKDRVARLALAY"
     gene            complement(5876..6835)
                     /locus_tag="VFF98_14585"
     CDS             complement(5876..6835)
                     /locus_tag="VFF98_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64909.1"
                     /translation="MDFQLTEDQRALVDAIQSILTDHAELPQDQRLGYAWHDPAVQAL
                     LADNGFLDAGRSIGPLEAALIQFETAQSLISIEAMGCGLVAPMALPELPLAGPVALVA
                     ERDVARAIRNLPVATTLLVDCGEDVMVLPVAAGQVEPVASIYGYPYGRFRAPVRLADG
                     QRLAGRGALLRQWWRTGLAVEIAGAARAAIDFTIDYVRQRQVFGRPVGAFQSVQHRLV
                     QRHGHARAGYFLAMRAAWSGDAGDADVAAAHALLGIKPLLFDLHQFNGGMGVTTEYAL
                     HFWTYRIRALQAEVGGLTGAALDIAARRWADADAPLPKGADDE"
     gene            complement(6839..7981)
                     /locus_tag="VFF98_14590"
     CDS             complement(6839..7981)
                     /locus_tag="VFF98_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64910.1"
                     /translation="MEFAWTDDQIAYRQQVRQALDELLPADWDETYVPESYASDLQVA
                     FSRVFCAQLAERGLLMPHWPKEFGGSDSPDWYHFILGEEMKAAGEPRGSQYMNVNWIG
                     PTLLKFGTEAQKAQHVQGIASGTVIWCQGFSEPNAGTDLAALRTRAERDGDAYVVNGS
                     KIWTSYARTADWCFLLVRTGPARKDVSILLVPMDTPGISVTSFPGLIKEGHLNEVFFT
                     DVRVPVANRLGDEGQAWAIVTHALAYERVGVPRYHTGLDALDLAVSALKADGRWEADP
                     VMRARAGTLVAQFEAARMLTYLVVDQRVRREPPNTDANLSRIVALEAVTEMMHFIADY
                     VPDCLAGGDRLLEDYYRINIPAGITGGTNEIQLDLVARRGLGLPGA"
     gene            complement(7998..9212)
                     /locus_tag="VFF98_14595"
     CDS             complement(7998..9212)
                     /locus_tag="VFF98_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU64911.1"
                     /translation="MSSVLFELQRLEPFRAEVRAWLAQNLPANWQAGMTGASEEAHVA
                     FQKAWFRKLMVPGYAAPHWPREWGGSGLSFAEQIVLYEEIARAGAPRLILYFIALHHV
                     PGTLLHWGSEAQRQRHLPAVLGGDEIWCQGFSEPGAGSDLAALRTRAVRKGDHYVVNG
                     QKTWSSNAHLARYCLLLARTDPDAPKTRGISYFILDLQTPGVTLRPIKQMTGASHFNE
                     IFLDDVAIPAENLIGPEHGGWAVAQSTLSSERGLSVLELAERMREGFKLLLAAARMVA
                     PGGRAPFEDDEIRRRLVDLRCRIEALRLLVNNQLSGVLRGASAGVEPSIIKLYYSDLL
                     RDFTELGVTIGGIEAQYLQAVLMGGANETGYWMQDYLYSWAWTISGGSSEIQRNIIAE
                     RGLKLPREPQAA"
     gene            complement(9433..10659)
                     /locus_tag="VFF98_14600"
     CDS             complement(9433..10659)
                     /locus_tag="VFF98_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZU64912.1"
                     /translation="MNEVWIIDAVRSPRGLGKAEGALAGVHPQHLFAQLLQALVARTG
                     AHGADIDDVIAGVNTQQGKQGLNLARMAVLDAGLPATVPGFSLDRFCGSGLTALSLAA
                     MGVLSGAQDLVIAGGVEMMSHTRTLAREFMMDSGNLELRAKVPQPHQGLCADLIATLE
                     GFTREELDGVALASQQRADRAIREGRFARSLVPVRDGAGAVVLDHEQFPRPQTTAAGL
                     AGLKPAFEAVFDRQIDADGTTFGTLVRRAYPDLALRYLHTAATSSGVVDGAGALLLAA
                     PAYARAHGLQPRARVRAVATVGDDPTLMLNGPVPAARKVLAKAGLALGDIDLFEVNEA
                     FACVPLKFMRDLGLDPEVVNVNGGAMALGHPIGATGAILAGTLLDELERQDKSLGLVT
                     MCAAGGLAPAMIIERI"
     gene            complement(10720..11493)
                     /locus_tag="VFF98_14605"
     CDS             complement(10720..11493)
                     /locus_tag="VFF98_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU64913.1"
                     /translation="MKLDGVAAVITGGASGLGEAAARALAAEGVKVALFDLNREKGAA
                     LAAELGGVFCEVDITSDASVEAGFAAARAAQGQERILVNCAGRGNTFKTAQRSRETGE
                     ISHFPIDQFEKIVLLNLVGTFRCIAKSAAGMLTLDPLGEDGERGCIVNTASVAATDGQ
                     AGQAAYASSKAGIVGLTLPIARDLRRDGIRVNTILPGIMDTPLFATARPELRQQLGDS
                     VPFPRRMGHPAEFASLAVEMCRNAYLNGEHVRLDGAIRM"
     gene            complement(11599..>12002)
                     /locus_tag="VFF98_14610"
     CDS             complement(11599..>12002)
                     /locus_tag="VFF98_14610"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZU64914.1"
                     /translation="AFSEQVGPFYACDEGLGPQEHGRFGFRVEPRHCNLRPMCHGGML
                     ATFLDVALARGLRLAGGMAPPLPTISLALDYLAPAPLGAWVEARVSVSRIGRSTGFVS
                     AMIYADDAPVLRGSGVFRHFLARQGVEGAGQ"
BASE COUNT         2126 a         4260 c         3789 g         1827 t
ORIGIN      
        1 gtgcgcatcg ccaaccgccg cctgctcgac atctacaccc gccaccgcct ggcgctgatc
       61 gaaatgcccc ggcaagtgcc ccgcatcgaa caggtcatcc gctacacctc gctggaaatg
      121 ctgaaccggg gcaacatccg cctctatctc agccagcatc ccgaattcga cagcgagaaa
      181 accgaaaacc acatcttcta catcgagacg atcatccacg cgctgatgcg ccgctatgtg
      241 ctggaggcgc ccgaaggcct gccgcccgac gagctggtgg agcagatctg cgaactcatc
      301 ctgtcctatc tgggccggca gcgcatcgat ccggtggccc gggaagcagg ccggccatga
      361 ccgaacctgc cggaaccgcc ccccgccgcg cggccaatgg cgcgctggaa gcgcagatca
      421 tcgaggcggc ctatgcctgt ttcgaacgct tcggcatcac caagacgacg atggaggata
      481 ttgcccgcgg cgcctctgtc tcgcgccagt cggtctatcg ctatttcgcc cgcaaggacg
      541 acatactcga cgaaatctgc gcgcgcgagg ccgtgaaaat caatgccatc gtgcgcgccc
      601 gcatccgccg cgacggcaat ttcgccgaca cgctgaccga tgccctgtgc gtgatcatca
      661 gcgaggccga caagaacccc tatctgcgcc aggtgatcga tgcgccctct gtgcaaacgc
      721 tggccagcag cccggccagc cgcgcctatc ggctgaacag atcctattgg cagggcatga
      781 tgcaacgcgc cgccgcggcc gggcagctgg cggatgatct ctcgctcgac gagatcgtct
      841 cctggctgct gatgatgcaa tcggtgctgc aattgcgcct ggccagcggg cagatcgatg
      901 ccgcccagct gcaccggctg gtgcgccgct ttgccgtggc gcccttgctg aaatcggtgt
      961 gagggcgccg cccggaacat aaggccggcg ccctggcggc ggggcgcata ggcgccctgg
     1021 cagtggagcg catacgcgcc ctggcggcgc tgcgcatgaa cgatttacat tttacgtttt
     1081 ttgtaacatg attcgcgcca gagaggttgc ccggcccgca agaggcggtc tgaccccagg
     1141 aaattgtctg acaaggagcg gatcatgctg aaatccacgc aggccccggc cgagcgcctg
     1201 ttgacccagg cccaaatgca gcagctggcc gggctggaag attcgaaccc ggcggaagtg
     1261 ttccgcgaga tcgaccatct gcccaccgac atctacgcct ccgaggcacg cttcgagctg
     1321 gaacggcaga agctgtttcg cgggcggccg gtgccggtgg gcgtgtcggc gctgctgccc
     1381 accgccaaat cgcatgtcgc catcgacgat tatggcactt ccctgctgct gacgcgcgac
     1441 gaggaagggc aggtgcacgc cttcttcaac gtctgcaccc accgcgcgat caagctgtgc
     1501 caggccaggg agccgcagaa aaaggggctg gtcgtgtgcc cctatcatgc ctggagcttc
     1561 gacatgaagg gccgcctgaa ggccctgccc cgcccggaaa tcttccccgg gctggacaag
     1621 agccaaatgg gcctggtcga actggaatgc gtggaggcag gcggcctcat ctgggtcaat
     1681 ctcgaccgtg agcgcaaggc ggatttcgcg ctggtgcgcg ggccgctggg cgccgatctc
     1741 gatgcctttg gccttggcca gcagaagatc tgccaccacc tgcgagtgga cgtgaaggcc
     1801 aactggaagc tgatcgtcga cagtttttcc gagaactatc atgtcacccg cctgcacgcg
     1861 caatcgctgg gcggcatgtt cgtcgatcgc aagacctcct gcgagatgat cggcgagcat
     1921 ctgcgggtga tgtcgggccg ggcggaattc cgccagggcg aggaaatcgc ttcctatgag
     1981 gccttccgca aatcggcggt ggcgcattac acgatcgtcg ccggcgcgct gatcatcacc
     2041 agccccagct atgtcagcgt gatgctgctc tcgccgcagg cggtggatca caccgtggtg
     2101 aactattaca tgatggttga ccaactgccc gagaccgagc gcgaaatcgc ccatttcgac
     2161 cgatcgctgg cgctgatgaa gcagatcacc accaaggagg atttctgggt ggccgaactg
     2221 ggcacgattg gcgccaaaag cggcgtcgtg ccgttcatga cctgcggcgg catggagcgc
     2281 gagatgatca ccttccacca gtcggtggat gccatcctcg ccggctgagg cagcccggac
     2341 gcagcgcgga tggggcggcg caggccgccc caagcgcagg ccgccccgtc cgttcagccc
     2401 agatgcgccg gccggccgtt caacaggatg gtcttgcttt ccagataggg cagcagccct
     2461 tccacgccgc cctcgcgccc cacgcccgat tgcttgaaac cgccaaaggc aatggtgaaa
     2521 tccaccagat ggccattgtg ccccacggtg ccgctgcgca gctggcgggc cacggcatag
     2581 gcgcgatcag gatcgttggt gaacaccgcc ccgttcagcc cgaaaggcga ggcattggcg
     2641 aggcgcacgg cgtcctcctc gtcctgcgcg gggatcaccg ccagcaccgg gccgaacact
     2701 tcctcgcggg ccagcgcgga ggcgttgtcc accccggcaa agacggtggg cgccacatag
     2761 aagccgcgct ccagatgggc cggccggccg ccgcctgcca ccagccggaa accatcggca
     2821 cgagcgcgct cgatatggcc cagcacgcgg tcccgatgga ggcgggtggc cagcggcccc
     2881 atctgcgtgg ccggatcgaa gggatcgccc accaccactt gcgagaactg cgcgccgatc
     2941 gcctcggcca tgcgctctgc ccggtggcgc ggcacgatga tgcgggtgag cgaggcgcac
     3001 acctgcccgg acatgtcgca ttcgcgcagc gccagttcgc gcgcggccgc ctcgaggtcg
     3061 tagtcgtcaa gcaggatcgc ggccgacttg ccccccagtt ccagcgtgta gcgcgccatg
     3121 cgcccaccca ggatcgaggc gatatgccgg ccggtggcgc tcgatccggt aaagccgatc
     3181 ttgtcgacat ccgggctgtt gaccagcgct tcggaggcct gccggtcggc gatcaccaca
     3241 ttcagcacgc cgcgcggcag gcccaccttc tcggccacct cggccatcag cagggcatgg
     3301 ccgggcgcct cgggcgatcc cttgatgacc acggtgcagc cggccagcag cgccggcgcc
     3361 accttgaagg cggtgatcat gatcggcccg ttccacggaa tgatcgcgcc caccacgccc
     3421 accggctcgc gcaccagcag gccaagggcg gcgccagaca ccgtcttgcg ctgctcctcg
     3481 aaggggaaac tgtcggccag atctgcgtaa tagcggaaaa aaccggcagc ccccggcgca
     3541 taggcctgcg ccatggaatg gaggatgccc atctcattgg gccagatttg ggcgatatcg
     3601 gccgcgcgcg cttccagttc cgcggccatc gcccgcagat aggccgcccg ctcggccggc
     3661 gccatgcgcg gccaggggcc ttcgtcgaaa gcggtgcggg cagcggcgat ggcccgctgc
     3721 acatcggcca cgccggcact ggccacctgg aaatagagcg cctcgctggc cgggcagatc
     3781 acgtcgatca ggccatcgcc ggcactgtct tgccaggcgc catcgacaaa gatctggcgg
     3841 gggtgcgaca ggggcacggc ctggtgcccc gatggaggta tcggcatgat cttatcctca
     3901 tccggcgggc gcttaaaagt tcgataatcg cccttgcgtt ctattatcgc caaggttagc
     3961 gataagcgcg acaaaggcaa gaaccaacag gagcatcagg atgacagcaa ggcgatcgat
     4021 tatcgtgacc ggtggcgcca gcggcattgg cgaggcatcg gcgcggctgg ccgccgcgcg
     4081 cggctgggca gtgaccatcg ccgatcgcaa cccggccggc gaggacattg ccgccgcgat
     4141 ccgcgccgag ggcggcgagg cctgcttcat cgccaccgac gtgaccgagg aagccagcgt
     4201 gcgcgcgctg gtggccggcg cccttgccgc ccatggggcc ctgcacggcg cgatcaacag
     4261 cgccgggatc gccggctgct cgcggccgat ccacgagatc gaactggccg actgggaccg
     4321 gctgcacgcg atcaacctgc gcggcatgtt cctttgcctc aagcaccagg tggcggcgat
     4381 gtgggaacag cgcgccggca gcatcgtcgc ggtttcctcg gcggcggcgc tgaagggcct
     4441 gccgtggagt tcggactatt gcggctccaa ggccgggatc gatggcatgg tgcgcggcgc
     4501 ggcgatggat tgcgccgagc agggcatccg catcaacgcg ctgctgcccg gccccacgct
     4561 gacgccgctg gcggtcggct cgtccaacgc caatccggcg ctggccaaga cccgcatccg
     4621 cccgatggaa cgcgtggccg acccgatcga gatcgcccgg gcggcggtat ggctggtgtc
     4681 ggatgaggcg agctttgtca ccggcatcgc catgccggtg gatggcggca tggttatcgc
     4741 ctgagccggc cgcagggcgc gcccaaggcc gcgccctgcc agctaatagg ccagcgccag
     4801 gcgcgccacc cggtccttgt gcgtggccga gctgccgaat agctgctggc tcagcttggc
     4861 gcgcttgaga aagaggtggc agggatgttc ccaggtgaag ccgatgccgc catgcagctg
     4921 caccgcatcg ccagagaaag cggcaaacac atcgccggca taagccttgg cccccgaggc
     4981 gatggccgat gcctccgcgc tgcccgcctg caacagggcc gcactgtggc gggccagcac
     5041 tttcaccgcc tcgatccgca ctttccagtc tgccatgcgg tgcttcagcg cctggaacga
     5101 gccgatcggc cggccgaact gttcgcgcat gcccagatag tccaccgtgc gctccagcag
     5161 cgcctcggcc gcgccaacgc agtcgcaggc aaggcccatc gccgcatgat cgagcaaggc
     5221 tgcccagccc tcctcgtcca ccgccagcac atcggccgcc actgcccctt ccagcacaat
     5281 gcgcgcgctg ctgcgggtga gatcgatcag cgggcgcggc gtgatcgcca gcccggccgc
     5341 gcctttgggc accagcgcca gcgccagcgc tccatcggcc tggcgcaccg gcagcagcag
     5401 cagatcggcc acatcggcaa aaggcacatg atcgacagcg ccggcgatcc gcccatcggc
     5461 agcgatctgt ggcagcgccg ccggcaaagt gatggctgcg ggcaaggcgg cggcctccgg
     5521 caaggcgatg gccgcaaggc agctgccatc tgccagccgc gccagccagc gcgcacgcgc
     5581 ctcggccccg ccatggcgca ccagcgcccc tgccgccagc atcgccggca gcaccggcac
     5641 cgaggccagt tcgcggccca gttcctcgta gagcaccgcc agatgctcca gccccaggcc
     5701 cagcccgccc tcttcctccg gcagcgccag gcccagccag ccaaggccgg ccatctcctg
     5761 ccacagcgcc cgatccagcc cacggccggc ctcatcgggg atggcgatca ccccgcccga
     5821 acggtcggcc agaacggtgc ggatcgcctc gcgcagctct tctgtgtcca gcatctcact
     5881 catcatcggc ccctttcggc agcggcgcat cggcatcggc ccagcggcgc gcggcaatat
     5941 ccagcgccgc gcccgtcaat ccgcccactt cggcctgcag ggcgcggatg cgataggtcc
     6001 agaaatggag cgcatattcg gtggtaaccc ccatgccccc attgaactgg tgcagatcga
     6061 aaagcagcgg cttgatgccc agcagggcat gggcggcggc cacatcggca tcaccggcat
     6121 caccgctcca ggcggcgcgc atggccagga aatagccggc gcgggcatgg ccatggcgct
     6181 gcaccagccg gtgctgcacc gactggaaag cgcccaccgg ccggccaaaa acctggcgct
     6241 ggcgcacata atcgatcgtg aaatcgatcg ccgcccgcgc ggccccggca atctccacgg
     6301 caaggccggt gcgccaccac tgccgcagca gcgcgccgcg cccggccagg cgctggccat
     6361 cggccaagcg caccggcgcg cgaaaccggc catagggata gccatagatc gaggccaccg
     6421 gttcaacctg ccctgccgcc accggcagca ccatgacatc ctcgccgcaa tcgaccagca
     6481 gcgtggtggc caccggcaga ttgcggatgg cgcgcgccac atcgcgctcg gccaccagcg
     6541 ccaccggccc tgccagcggc agttcgggca aggccatggg cgctaccagg ccgcagccca
     6601 tcgcctcgat cgagatcagg ctctgggcgg tttcgaactg gatcagcgcc gcttccagcg
     6661 ggccgatgct gcgcccggca tccagaaagc cattatcggc cagcagcgcc tgcacggcgg
     6721 gatcgtgcca ggcatagccc agccgctggt cctgcggcag ttcggcatga tcggtgagga
     6781 tcgactggat cgcatccacc agcgcgcgct ggtcttcggt cagctggaaa tccatcggtc
     6841 aggctcccgg caggccaagg ccgcgccggg cgaccagatc gagctggatc tcgttggtgc
     6901 cgccggtaat gccggcggga atgttgatcc ggtaatagtc ttccagcagg cgatcgccgc
     6961 ctgccaggca atcgggcaca taatcggcaa taaaatgcat catctccgtc accgcctcca
     7021 gcgcgacaat acgggacagg ttggcatcgg tgttgggcgg ctcgcgccgc acccgctgat
     7081 ccaccaccag ataggtcagc atccgcgccg cctcgaactg ggccaccagc gtgcccgcgc
     7141 gcgcgcgcat caccggatcc gcctcccagc gcccatcggc cttcagcgcc gagaccgcca
     7201 gatccagcgc atcgagcccg gtgtgatagc gcggcacgcc cacccgttca taggccagcg
     7261 catgggtgac gatggcccag gcctgcccct catcgcccag ccggttggcc accggcaccc
     7321 gcacatcggt gaagaacacc tcgttgaggt ggccctcctt gatcagcccg gggaagctgg
     7381 tgaccgaaat gcccggcgta tccatcggca ctaacagaat gctgacatcc ttgcgcgcgg
     7441 ggcccgtgcg caccagcaga aagcaccagt cggccgtgcg cgcatagctt gtccagatct
     7501 tcgagccatt gacgacatag gcatcgccat cgcgctcggc gcgggtgcgc aaggcggcca
     7561 gatcggtgcc ggcattgggc tcggaaaacc cctggcacca gatcaccgtg ccgctggcga
     7621 tgccctgcac atgctgcgcc ttttgcgcct ctgtgccgaa tttcagcagg gtggggccaa
     7681 tccagttgac attcatatac tgcgagccgc gtggctcgcc ggccgccttc atctcctcgc
     7741 ccaggatgaa atgataccag tccgggctgt cgctgccgcc aaattccttc ggccagtgcg
     7801 gcatcagcag gccgcgttca gccagctgcg cgcaaaacac gcgcgagaag gccacctgca
     7861 aatccgaagc atagctttcc ggaacgtagg tctcgtccca atcggccggc agcagttcat
     7921 ccagcgcctg ccggacctgc tggcgatatg cgatctgatc gtctgtccag gcgaattcca
     7981 tcgatccaat aaacccctca ggccgcctgc ggttcacgcg gcagcttcaa tccccgttcg
     8041 gcgatgatgt tgcgctggat ttccgaactg ccgccggaaa tcgtccaggc ccaggaatag
     8101 agataatcct gcatccaata gccggtctcg ttggcgcccc ccatcagcac ggcctgcaga
     8161 tattgcgcct cgatgccgcc gatggtcacg ccaagctcgg tgaaatcgcg cagcagatcg
     8221 ctgtagtaga gcttgatgat ggagggctcg accccggccg aggcgccgcg cagcacgccc
     8281 gaaagctggt tgttcaccag caatcgcagc gcctcgatgc ggcagcgcag atccaccagc
     8341 cggcggcgga tctcgtcatc ctcgaacggc gcccggccgc ccggcgccac catgcgggcg
     8401 gcggccagca gcagcttgaa accctcacgc atgcgctcgg ccagttccag caccgacagc
     8461 ccccgctccg aggagagggt ggattgcgcc accgcccagc cgccatgctc cgggccgatc
     8521 aggttttccg ccgggatcgc cacatcatcg aggaagatct cgttgaaatg ggaggccccc
     8581 gtcatctgct tgatcggccg cagcgtaacg ccgggcgtct gcagatcgag gatgaaatag
     8641 gaaatgccgc gcgttttcgg cgcatccgga tcggtgcgcg ccagcagcag gcaatagcgc
     8701 gccagatggg cgttggacga ccaggtcttc tgcccgttca cgacataatg atcgcccttg
     8761 cgcaccgccc tggtgcgcaa ggcagccaga tccgatcccg cgcccggctc ggaaaagccc
     8821 tggcaccaga tctcgtcgcc gcccagcacc gccggcagat gccgctggcg ctgggcctcg
     8881 ctgccccagt gcagcagcgt gcccggcaca tgatgcaggg cgatgaaata gagaatgagg
     8941 cgcggcgcgc cagcacgggc gatttcctcg tagagcacga tctgctcggc aaaggaaagg
     9001 ccactgccgc cccactcgcg cggccagtgc ggcgcggcat agccgggcac catcagcttg
     9061 cggaaccagg ccttctggaa ggcgacatgg gcctcctcgc tggcgccggt catcccggct
     9121 tgccagttgg ccggcaggtt ctgcgccagc cacgcgcgca cttcggcgcg aaacggctcc
     9181 agtctctgca attcgaacag aacgctgctc atcttcaccc tctcgccgac aggacccgaa
     9241 gggtcggtca ggcccgcctt gccggccggt tctttttgcc gccccacaca tgttgtgcga
     9301 taaacgatct attgtgccat atgtaaccga tgtgcccttt gccgccccat gtcaaccatg
     9361 gaaatgcgct ggcgcacaaa tatgtgctat aaacgccctt atgatctata ttcgaccata
     9421 agggcgcggt tcctatatcc gttcgataat catcgccggg gccaggccgc cggccgcaca
     9481 catggtgaca aggccaaggc tcttgtcctg ccgctccagc tcgtcgagca aggtgccggc
     9541 caggatcgcg ccagtggcgc cgatgggatg gcccagcgcc atcgcgcccc cgttcacatt
     9601 caccacctcc ggatcgagcc cgagatcgcg catgaacttc agcggcacgc aggcaaacgc
     9661 ctcgttcacc tcgaacagat caatatcgcc cagcgccagg cccgccttgg ccagcacctt
     9721 gcgcgcggcg ggcaccggcc cgttcagcat cagcgtggga tcatccccca ccgtggccac
     9781 tgcccgcacc cgcgcccgcg gctgcagccc atgcgcccgg gcataggcgg gcgcggccag
     9841 cagcagcgcg ccggcgccat caaccacccc cgaactcgtt gccgccgtgt ggagatagcg
     9901 cagggccaga tccggatagg cccggcgcac cagcgtgccg aacgttgtgc catcggcatc
     9961 gatctgccga tcgaacaccg cctcgaaagc gggtttcagc ccggccagcc cggcagcggt
    10021 ggtctgtggg cgggggaact gctcatgatc gagcaccacc gcccctgcgc catcgcgcac
    10081 cggcaccagc gagcgggcaa agcggccctc gcgaatggcc ctgtcggccc gctgctggct
    10141 ggccagtgcc acaccgtcca gttcctcgcg ggtgaagcct tccagcgtgg cgatcagatc
    10201 ggcgcacagg ccctggtgcg gctgcggcac cttggcccgc aattccagat tgcccgaatc
    10261 catcatgaat tcgcgcgcca gcgtgcgggt gtgcgacatc atctccaccc cgccggcaat
    10321 caccagatcc tgcgcgcccg acagcacccc catcgccgcc aggctcagcg ccgtcagccc
    10381 cgagccgcag aagcgatcga gcgaaaagcc gggcaccgtg gccggcaggc cggcatccag
    10441 caccgccatg cgggccaggt tcaagccctg cttgccctgc tgcgtgttca ccccggcgat
    10501 cacatcgtcg atatcggcgc catgggcccc ggtgcgggct accagcgcct gcagcaattg
    10561 cgcgaacaga tgctgcgggt gcacgccggc cagcgcgcct tccgccttgc ccagcccgcg
    10621 cgggctgcgc accgcatcga tgatccaaac ctcgttcata tcgcttatcc atgccaagag
    10681 tggggccaag agggagatcg ggcgatgatc gcccgccggt cacatccgga tcgcgccatc
    10741 cagccgcaca tgctcgccgt tcagataggc gttgcggcac atttccaccg ccaggctggc
    10801 gaattcggcc ggatgcccca tgcggcgcgg aaacggcacg ctgtcgccca gttgctggcg
    10861 cagttcgggg cgggcggtgg caaacagcgg cgtatccatg atgcccggca ggatcgtgtt
    10921 cacgcggatg ccatcgcgcc gcaaatcccg cgcgatcggc agcgtgaggc caacgatgcc
    10981 cgccttggag gaggcatagg ccgcctgccc ggcctgcccg tcggtggcgg ccacagaggc
    11041 ggtgttgacg atgcagccgc gctcgccgtc ctcgcccagc ggatcgagag tcaacatacc
    11101 ggccgccgat ttggcgatgc agcggaacgt gccgaccagg ttgagcagca cgatcttctc
    11161 gaactgatcg atggggaaat ggctgatctc gccggtctcg cgcgagcgct gggcggtttt
    11221 gaacgtgttg ccgcggccgg cgcagttaac aaggatgcgc tcctgcccct gcgccgcccg
    11281 ggccgcggca aagcctgcct ccaccgacgc atcgctggtg atgtccacct cgcagaacac
    11341 tccgcccagt tcggcggcca acgccgcgcc cttctcgcgg ttgaggtcga acagcgccac
    11401 cttcacgcct tctgccgcca gcgcgcgggc cgccgcctcg cccaggccgc ttgccccgcc
    11461 ggtgatcacc gccgccactc catcaagctt catcgtctgc tcctgcccac attccgctgc
    11521 gttttattgt gcgttaatcg ccccaataag cgaatatcga tctttatggc agagcgccat
    11581 ggcaatgaac agggggtttc attggcccgc cccttcaacg ccctgccgcg ccaggaaatg
    11641 gcggaacacc ccgctgccgc gcagcacggg cgcatcatcg gcatagatca tcgccgagac
    11701 aaagccggtg ctgcgcccga tccggctgac cgagacccgc gcctccaccc aggcccccag
    11761 cggcgccggc gccagataat ccagcgccag gctgatggtg ggcagcggcg gcgccatccc
    11821 gccggccaga cgcagcccgc gcgccaaggc cacatcgagg aaagtggcca gcatgccgcc
    11881 atggcacatc ggccgcaggt tacagtggcg cggctccacg cgaaagccga agcggccgtg
    11941 ctcctgcggg cccaggccct cgtcgcaggc atagaacggg cccacctgtt cggaaaaggc
    12001 gc
//
LOCUS       DASRYE010000285         5093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_158792,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000285 DASRYE010000000
VERSION     DASRYE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158792"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1624)
                     /locus_tag="VFF98_14615"
     CDS             complement(167..1624)
                     /locus_tag="VFF98_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64915.1"
                     /translation="MTSRLAIDEGDFVPPVEIPLPEERPAPAAAYPRKLSRADLVTYG
                     LAAFGPGLLQSPLTLYVPQLYAKVYGIPLAALGTALVVLRIVNAFTDQLVGYISDRTR
                     TRWGARKPWIAVGAALTLVAAFFLLRPGPEVGLAYLVGWKIVYDFAYTVTDINYTAWG
                     AELSPEYAMRARITGIRGFVFNIGNLCNDALPIILAWAGLVATSAYSVPVLGYFFVVA
                     VVVIPTTTAFSLARAPRGVALPPERPDFWSFLRSVRGNRPLWLYLGAFCLGGMGLGVL
                     QLMFTFYDGYMRMGAWYPWLMTVFAITMAFTTPVWSWLAGRIGKHRAYVVSVVIVSLA
                     TQGYWFLDPVGMSKTLVVGVSMVIVFFIGMGASAVLVLSPAILADVVDYGRLKTRQQR
                     TGGYYAFYMLTSKISMAIGAGLGFMALSAFGYNAKAGAVNTGTAAFGMLFTVALMPAI
                     LKVGGGLVIWNFPIDRRRHRIIERRLATLAARAAR"
     gene            complement(1694..2986)
                     /locus_tag="VFF98_14620"
     CDS             complement(1694..2986)
                     /locus_tag="VFF98_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU64916.1"
                     /translation="MDSAVDTGIAPSRGALDARGVAGFWASNRALITITLVNFVSLAG
                     FGLMFPVFATYGRQIGASGMEIAGTVAVFSFGQFLSSPAWGWLSDRHGRRPVLIWGLV
                     GGAAIYVLHVWAVTPLLLVLVRFISGMASGCFSVGFAVASDISTPATRTRVMGVVGAG
                     FSLGFIFGPAIGGVTASISGQEHAFALVSVVGAVMGLLAGLTCWAFLPETTTRAEMES
                     TSAPGTLALLRRPGFAAPVALALLSAAGFAKMEAVLGLFADDVLGLRPLGIGVMFGFM
                     GLVTTATQLLLTGRVARIMGERRMLLASLAVIGVGMIVLGCAKGLVVAGLGLVFTSAG
                     FALLNPALSGLTSLASPHDAQGAGLGLMQAASALGRVAGPAAAGVLYDAQGPAAPMFW
                     AGAIFLAALAVAPFLPMPGAATTAGEGDDGAPSRSTDG"
     gene            complement(3106..4101)
                     /locus_tag="VFF98_14625"
     CDS             complement(3106..4101)
                     /locus_tag="VFF98_14625"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZU64917.1"
                     /translation="MLTTGVAATPASAQTQVQDATSPGSASAPASTSAPAGGAEASAP
                     AGQGAAQAPAQPNAPANAAPAPQGDSGGIVVAGRGPPPPTDPARALNLKSYAVVEGAD
                     KLMVGPAAHTYERGLPTPLRDGFHNAIYNLREPANAVNFMLQHRVGKSLAATARFAIN
                     STVGLAGFFDVAKRKPFRIPYRPNGMADTLGYYGVKQGPYMFLPIIGPTTVRDLVGTT
                     IDRFWFPLVAGFPLDTFEFGIAMTVVNGLDDRTRIDPLLRKFREESGDPYTAHREFYL
                     KRRQAEIDGLHKHPRNAPELAPPPPFVPAPVPEPAPVPAPAPATPEAAPVPAPAG"
     gene            complement(4445..4918)
                     /gene="ybaK"
                     /locus_tag="VFF98_14630"
     CDS             complement(4445..4918)
                     /gene="ybaK"
                     /locus_tag="VFF98_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HZU64918.1"
                     /translation="MSKATRATRQLDAAKVAYAVHAYDYDGDVERIGMAAAAALGVDA
                     AKVFKTLMVLADGKPACVILPADREVAMKKLAAALGAKQAQMMPIADAERISGYKVGG
                     ISPFGQTRRLAVVIDEAAQAQERIFLNGGQRGLQVELAPGDAARVLDARLAAITA"
BASE COUNT          949 a         1829 c         1607 g          708 t
ORIGIN      
        1 cccgcccctc tccagctcgg aaactcgcgg ccccgaagcg gcatgaagcg gcgctgcggg
       61 gcaagccttg gcccaatggc cggcttgacg cagccacctt atggtagagt gagcgctttg
      121 accagcgcaa ttgtgttcac ttcatggcat gccggaacac agcccatcag cgggcagcgc
      181 gcgcggccag cgtcgccagg cgccgctcga tgatgcgatg gcgccgccgg tcgatcggga
      241 agttccagat gaccagcccg ccacccacct tcagaatcgc cggcatcagc gccacggtaa
      301 acagcatgcc aaaggccgcc gtgccggtgt tcaccgcgcc cgccttcgca ttatagccaa
      361 acgcgctgag cgccatgaac cccagccccg ccccgatcgc catcgagatc ttgctggtca
      421 gcatgtagaa ggcataatag cccccggtgc gctgctggcg ggttttcagc cgcccataat
      481 cgaccacatc ggccagaatg gcaggcgaga gcaccagcac ggcgctggcc cccatgccga
      541 tgaaaaacac gatgaccatg ctgacgccca ccaccagcgt cttggacatg cccaccgggt
      601 cgaggaacca gtagccttgc gttgcaaggc tgacgatcac caccgagacg acataggccc
      661 ggtgcttgcc gatccgcccg gccagccacg accagaccgg ggtggtaaag gccatggtga
      721 tggcaaacac cgtcatcagc caagggtacc atgcgcccat ccgcatatag ccgtcgtaga
      781 aggtgaacat gagctggagc acacccagcc ccatgccgcc aaggcagaaa gcgcccagat
      841 aaagccacag cggccggttg ccccgcaccg agcgcaggaa gctccagaaa tcgggccttt
      901 cgggcggcag ggcaacaccg cgcggcgcgc gcgccaggct gaaggcggtg gtggtgggaa
      961 tgaccaccac cgccaccacg aagaaatagc ccagcaccgg caccgaatag gccgaagtcg
     1021 ccaccagccc ggcccaggcc agaatgatgg gcagcgcatc gttgcacaga ttgccgatgt
     1081 tgaacacgaa gccgcgaata ccggtgattc gcgcccgcat cgcatattcg ggcgacagtt
     1141 cggcgcccca ggcggtatag ttgatgtcgg tcacggtata ggcaaaatca tagacgatct
     1201 tccagcccac cagataggcg aggcccacct ctgggccggg ccgcagcagg aagaaggccg
     1261 ccaccagcgt gagcgccgcg cccaccgcga tccacggctt gcgcgcgccc cagcgggtgc
     1321 gggtgcggtc cgagatgtag cccacgagct ggtcggtaaa ggcgttgacg atacgcagca
     1381 ccaccagcgc cgtgcccagc gccgccagcg gaatgccata taccttggcg taaagctgcg
     1441 gcacgtacag cgtgagcggg ctttgcagca ggcccgggcc gaaggcggcc agcccatagg
     1501 tgaccagatc ggcgcgcgag agcttgcgcg gataggcggc agcgggcgcg gggcgctctt
     1561 cgggcaaggg tatctcgacg ggcggcacga aatcgccctc atcaatcgcc agcctgctcg
     1621 tcattgcact ctcctgcccg tgatggggca tcaggccgca gcaaccggca actgtcgacg
     1681 aaagatcgcg cccttatcca tctgtcgaac gagacggcgc gccatcgtcc ccctcgcccg
     1741 ccgttgttgc cgcgcccggc atgggcaaaa atggcgccac cgccagcgcc gccagaaaga
     1801 tcgcgccggc ccagaacatg ggcgcagcag gcccctgcgc gtcatacagc acgccggccg
     1861 ccgccggccc tgccacccgg cccagcgcgc tggcggcctg catcagcccc agccccgcgc
     1921 cttgcgcatc atgcgggctg gccagcgagg tcagcccgct gagcgccgga ttgagcagcg
     1981 caaaaccggc cgatgtaaac accagcccca gcccggccac caccagcccc ttggcgcagc
     2041 ccagcacgat catgccaacg ccaatcaccg ccagcgaggc cagcaacatg cgccgctcgc
     2101 ccatgatccg cgccacgcgg cccgtcagca gcaattgcgt ggccgttgtg accagcccca
     2161 tgaaaccgaa catcacgcca atgcccagcg gccggagccc cagcacatcg tctgcgaaca
     2221 ggcccagcac ggcctccatc ttggcaaaac cggcggccga aagcagcgca agggccacag
     2281 gcgcagcaaa accgggccgc cgcagcaggg ccagcgtgcc gggcgcgctg gtgctttcca
     2341 tttcggcgcg ggtggtggtt tcgggcagga acgcccagca ggtaagcccg gccagcaggc
     2401 ccatgaccgc gcccaccacg ctcaccagcg caaaggcatg ctcttgcccg ctgatgctgg
     2461 ccgtaacccc gccaatggcc gggccaaaga tgaagcccag cgagaagccg gcccccacaa
     2521 cgcccattac ccgcgtgcgc gtggcgggcg tcgaaatgtc ggacgccacg gcaaaaccca
     2581 ccgagaagca ccccgaggcc atgccgctga taaagcgcac gagcaccagc agcagcggcg
     2641 tgaccgccca tacgtgcagc acatagatgg cagccccgcc caccagcccc cagatcagca
     2701 ccggccgccg cccgtgccgg tccgacagcc agccccacgc cgggctcgag aggaactggc
     2761 cgaatgaaaa gaccgcgacc gtcccggcaa tttccatgcc cgaggcgccg atctgccggc
     2821 cataggtggc gaataccgga aacatcaggc caaagccggc cagcgacacg aagttgacca
     2881 gcgtgatcgt gatcaacgcc cggttgctgg cccaaaatcc ggcgaccccg cgcgcgtcaa
     2941 gcgccccgcg cgagggcgca attcctgtat caacggcgct gtccattacc ctgcccacct
     3001 ttgctccggc cggtgcctag gcacaaagcg cgggcgggcg ccatcgcggc cgccgcccct
     3061 gttcgcaggg cgacgacacg catgggcaaa gcagccttgg ccggttcagc ccgcaggcgc
     3121 aggtacaggt gccgcctcgg gcgtggcagg cgcgggcgct ggcacaggcg ctggctctgg
     3181 cacaggcgcc ggcacgaaag gcggcggcgg cgccagttcg ggggcgttgc gcgggtgctt
     3241 gtgcaaaccg tcgatctcgg cctgccggcg cttgagatag aactcgcggt gcgcggtata
     3301 gggatcgccc gattcctcgc ggaacttgcg cagcagcggg tcgatgcgcg tgcgatcgtc
     3361 cagcccgttc accaccgtca tggcaatacc gaactcgaac gtgtcgagcg ggaaaccagc
     3421 caccagcggg aaccagaaac ggtcgatcgt ggtgcccacc agatcgcgca ccgtggttgg
     3481 gccaatgatg ggcaggaaca tgtacggccc ctgcttcacc ccataatagc ccagcgtatc
     3541 ggccatgccg ttgggccgat agggaatgcg gaaaggcttg cgtttggcca cgtcgaagaa
     3601 cccggccagg cccaccgtgc tgttgatggc aaagcgcgcc gtggccgcca gcgacttgcc
     3661 cacgcggtgc tgcagcatga agttcaccgc attggccggc tcgcgcaggt tgtagatcgc
     3721 gttgtggaag ccatcgcgca gcggcgttgg caatccgcgc tcgtaagtgt gcgccgcggg
     3781 gccaaccatc agcttgtcgg cgccctcgac cacggcatag cttttcaggt tcagcgcgcg
     3841 cgccggatcg gtgggcggcg gcgggccgcg cccggctacc acgatgccgc cgctgtcgcc
     3901 ctgaggggct ggagcggcat ttgccggcgc attgggctgg gccggagctt gcgccgcccc
     3961 ctgccccgct ggagcgctgg cttcagcgcc gccggccggg gcgctggtgc tcgccggcgc
     4021 gctggcgctg ccagggctgg tcgcatcctg cacctgcgtt tgcgcgctgg ctggcgttgc
     4081 cgccacacct gttgtcaaca tggccgcaat cgcccccccc agagcgattc ctggccgaag
     4141 gcgtgtgccg ccctgtgccc ggtcctggca ttccccccgc acctcgctcc tgcgctgacc
     4201 acggcgacca ggagcggcaa aacggcgcga aatcattgca ataagatacc tttttcgagc
     4261 ggttgagcca atgttgaccc atgccgcgcc gccaatgcgc gggcctgccc ggccctcgcg
     4321 ccaacagcgc tgcatagccc ttagcaagaa gccccgccaa gggaaacgct acgttcgtca
     4381 gtctgtgtcg caacgcggca tttgcctgcc atccgttgcc caacgggcac gccggccggc
     4441 acgctcacgc cgtgatcgcg gccaggcgcg catccagcac ccgcgccgca tcgccgggcg
     4501 ccagttccac ctgcaacccg cgctgcccgc cgttcaggaa aatgcgctcc tgcgcctgcg
     4561 ccgcctcgtc gatcaccacc gccaggcggc gggtctgacc aaacgggctg atcccgccca
     4621 ccttgtagcc ggatatgcgt tcggcatcgg caatgggcat catctgggcc tgcttggcgc
     4681 ccagcgcggc ggccagtttc ttcatcgcca cttcgcggtc ggccggcagg atgacgcagg
     4741 ccggcttgcc atcggccagc accatcagcg tcttgaacac ctttgccgca tccaccccca
     4801 gcgccgcggc agccgccatg ccgatccgct cgacatcgcc gtcgtaatca taggcgtgca
     4861 ccgcataggc gacctttgcg gcatccaact gacgcgtggc gcgggtggct ttggacatgg
     4921 cattctccgg ctgggccagc gggcggggct acaggggcgc cataggccag ccagcgctgc
     4981 gccaccagtc cccgatcctg tgctcgccca acggccaaac ggcgcgtttt tctttgacaa
     5041 agccggcaag acaccggatg accggggccg gcttggcagt cttgaaaagc atg
//
LOCUS       DASRYE010000286         7134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_168907,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000286 DASRYE010000000
VERSION     DASRYE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168907"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="VFF98_14635"
     CDS             complement(<1..2278)
                     /locus_tag="VFF98_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64919.1"
                     /translation="MSGYRLPAGGRVNRAAPLPFTFDGRPYTGLAGDNLASALLASGV
                     MLLGRSFKYHRPRGLLGMGVEEPNALVNVNRGPARRTPNLRAPSVPLYEGLEAGSQNR
                     FPNLDFDFGALNDKLGAFFPAGFYNKTFMWPRSFWEKLYEPVIRRMAGLGESPLARDP
                     DHYASIYAHCEVLVVGAGPAGITAALEAAQKGGRVILIDEQDEIGGGALADPAQWAWL
                     DDARAKLEAAGVRVLARTTAFGYYHQNFIGAVERLTDHLTPAEARGAREKLWRIRAGE
                     VVLAQGAIERPLVFDGNDVPGVMLMSAARTLALRYGVAVGRSAVVMAVHDSGWTDALA
                     LVKAGVTVSAIIDLRSNVAPALLEAARAAGIAIHLGSAVTGVKGRKAITGLTFAAGGT
                     GRPQALACDALLMAGGWTPSLHLWSHAKGSMEWSAEWGAYVPGKAIEKCRAVGAGAGA
                     WDFGQGIIVSSLPTSKRPHQQKGFVDFQNDVKARDIRLAVQEGFRSIEHIKRYTTNGM
                     ATDQGKTSNLNALQLASEALGRPVTQIGLTTFRPPYSPQTFGALMGHHKDGLFQPLRK
                     SPIDGWATEQGAVFENVSTWRRARYFPRGGEDMHAAVNRECVAVRSGVGIFDASTLGK
                     IEVVGPDAAEFLNRMYTNPWKSLKPGRCRYGLLLREDGFITDDGVSARLSEDRFHLTT
                     TTGGAPRVLAMMEDYLQTEWSDLDVWLTSATEQWAVIALQGPRARDVIAPLVAGIDLS
                     AEAFPHMAIREGTICGVPT"
     gene            complement(2275..2547)
                     /locus_tag="VFF98_14640"
     CDS             complement(2275..2547)
                     /locus_tag="VFF98_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZU64920.1"
                     /translation="MILIHCPYCGEDRPEVEFVYGGEAHIARPLDPSGQTDPEWESYL
                     YIRANVRGRHHERWRHTHGCGRFFNAVRDTVTDLFETTYKAGEPRK"
     gene            complement(2561..3814)
                     /locus_tag="VFF98_14645"
     CDS             complement(2561..3814)
                     /locus_tag="VFF98_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZU64921.1"
                     /translation="MKRFSALNLFAEALKGHQGWPEHWPEKEPKPAYDVIIVGAGGHG
                     LGAAYYLAAKYGITNVCVIEKGWLAGGNAARNTTIIRSNYLYDESAHLYDHALKLWDG
                     LSHELNYNTMYSKRGVLMLAHNVHDIQSFKRHIHANRLNGVDNEWLSPEECKAYCPPL
                     DINPNARHPVLGGALQRRGGIARHDSVAWGYARKAVALGVDIIQNCAVTGIRRGPDGA
                     VQGVETEKGFIAAKKVGVSAAGNTSVVMNMAGLRFPLESYPLQALVSEPVKPIFPCVV
                     MSNTVHAYISQSDKGELVIGAGTDQYVSYSQRGALHIIEHTLAAICEIFPIFTRMRML
                     RSWGGIVDVTPDRSPILGKTPVPGLYVNCGWGTGGFKATPGAADLLAYTIAHDQPHRI
                     NAPFTLDRFRDGRLIDEAAAAAVAH"
     gene            complement(3858..5084)
                     /locus_tag="VFF98_14650"
     CDS             complement(3858..5084)
                     /locus_tag="VFF98_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU64922.1"
                     /translation="MNEFDSIAALLDQNRPGYTLPRELYVSEEAFRFDTEVMLKSVWL
                     YACTIAHVKNPGDYFVFELANNSVVILKDKEGEIRAFYNTCTHRGAKLCEHQKGHMGR
                     VMCPYHFWTFGLDGKLIAARGMPEGFEKDAHGLRPVHLENVGGLLFVCLADNPPPIDR
                     VKADIGAQLGLYNVGKLKVAAQSDLIDNANWKLVMENNRECYHCASNHPELLNSLSGD
                     GFGKGNPEDGVAAGADADVGDEIARWKDLGIHHDLVEFPDNWWHRIARIGLANGSLSQ
                     TLNGQPASQKLIWNVDHAEPTSLSIWTHPNSWHHFCCDHVITFSVLPIAPDKTLVRTS
                     WLVHEDAVEGEDYTVENLTAVWNATNQQDRKLAELNHAGIATDGYRPGMYALEENLVD
                     AFKTFYVEKAQAALGA"
     gene            complement(5090..>7134)
                     /locus_tag="VFF98_14655"
     CDS             complement(5090..>7134)
                     /locus_tag="VFF98_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64923.1"
                     /translation="ALAKGARQRGATIYRNTTVLGITQKANGEWVVTTDQGEITCEHV
                     VSATGSFARQTGAMVGLDIPVVPVEHQYIVTEQHPAIMERKAKGLPEMGVLRESDASW
                     YMREEAGGLLLGPYEIGAPACYVKGPAANSEYELFPDALERLEPYILSAIERVPAFGE
                     VGVKKVYNGAIAYTPDGSPIVGPAWDKKNFWLNEGHSFGITAAGGAGWQLAEWIVNGE
                     PSIDMMGVDPRRFGEYAGEGYLVEKNEEAYAKVFAIHYPDEEREAARALRRVPCHDRM
                     KALGAVFGSVYGWERPGWFAPEGYELSEADLDKADVILNENHPVVPGETIREKWSFRR
                     SNYFDFVGAEVRHVTEKVGIQDMSPFAKCQISGPGAEEWLNGLLTNAVPKKTGRVSLS
                     YLLTDNGGVRAEFTVYKKGPQQYYLVSAGALERHDHDYLLKAMPTDGSVRYERLTTAM
                     GVLVLAGPKSRDVLAKLTNTDLSNEAFPWLTGKTISIGAARVEALRVNFIGELGWEIH
                     HPIEMQNYIFDKLMAAGAEFDIRPFGIKAMIAMSIEKSYKLIPREMSIEYAALESGLE
                     RFISFKKPDFKGRSGLLARKEKGLAWQFVTLEVKDVTDADARGSEPIFKDGALVGRVT
                     SGGYGWRCGKSLALAMVRPDHAAVGTELEITILGTSFKAVVIADSPFDPENTALRS"
BASE COUNT         1188 a         2331 c         2345 g         1270 t
ORIGIN      
        1 gggtgggcac gccgcagatg gtgccctcgc ggatcgccat atgcgggaag gcctcggccg
       61 acaggtcgat ccctgccacc agcggcgcga tgacatcgcg cgccctgggg ccctgtaggg
      121 cgatcaccgc ccactgttcg gtggccgagg tcagccagac gtcgagatcg ctccactcgg
      181 tctggagata gtcctccatc atcgccagca cgcggggcgc gccgcccgtg gtggtggtga
      241 ggtggaagcg atcctccgac aggcgggcag aaacgccgtc atcggtgatg aagccatcct
      301 cgcgcagcag caggccatag cggcagcggc ccggcttcag gctcttccac gggttggtat
      361 acatgcggtt gaggaattcg gcggcatcag ggccaaccac ctcgatcttg cccagggtgg
      421 aggcatcgaa aatgcccacg cccgagcgca ccgccacgca ttcgcggttg accgcggcat
      481 gcatgtcttc cccgccgcgc gggaaatagc gcgcgcggcg ccaggtgctg acattctcga
      541 acaccgcgcc ctgttcggtg gcccagccat cgatcggcga tttgcgcagc ggctggaaca
      601 ggccatcctt gtggtggccc atcagcgcgc cgaaggtctg cgggctgtag ggcgggcgga
      661 aggtggtgag gccaatctgc gtgaccggcc ggcccagcgc ttccgatgcc agttgcaggg
      721 cgttgagatt gctcgtcttg ccctggtcgg tggccatgcc gttggtggtg tagcgcttga
      781 tgtgctcgat cgagcggaag ccttcctgca cggcaaggcg gatatcgcgc gccttcacat
      841 cgttctggaa gtcgacaaag cccttctgct ggtgcgggcg cttgctggtg ggcaggctgg
      901 agacgataat gccctgcccg aaatcccacg cgccggcgcc agcgcccacc gcgcggcact
      961 tctcgatcgc cttgcccggc acataggcgc cccactcggc gctccattcc atgctgccct
     1021 tggcgtggct ccacagatgg aggctgggcg tccagccgcc ggccatcagc agcgcatcgc
     1081 aggccagcgc ctgcggccgg ccggtgccgc ccgcggcaaa ggtgaggccg gtgatcgcct
     1141 tgcgcccctt caccccggta acggcagagc ccagatggat ggcaatgccg gccgcgcggg
     1201 ccgcttccag cagggccggg gccacattgc tgcgcagatc gatgatcgcg ctgacggtga
     1261 cgcccgcctt gaccagcgcc agcgcatccg tccagccgct gtcgtgcacg gccatgacga
     1321 ccgcgctgcg gcccaccgcc acgccatagc gcagcgccag cgtgcgcgcc gcgctcatca
     1381 gcatcacgcc gggcacatca ttgccatcaa acaccagcgg gcgttcgatg gcgccctggg
     1441 ccagcactac ctcgcccgcg cggatgcgcc acagcttctc gcgcgcgccc cgcgcctcgg
     1501 ccggggtcag gtgatcggtc aggcgctcca ccgcgccgat gaagttctgg tgatagtagc
     1561 caaaggccgt ggtgcgggcc agcacgcgca cgccggccgc ttccagcttg gcccgggcat
     1621 cgtccagcca ggcccactgg gccggatcag ccagcgcgcc gccgccgatc tcgtcctgct
     1681 cgtcgatcag gatgacgcgg ccgccctttt gcgccgcttc cagcgcggcg gtgatgccgg
     1741 caggcccggc gcccaccacc agcacctcgc aatgggcata gatgctggcg tagtgatcag
     1801 ggtcgcgcgc cagcgggctt tcgccaaggc cggccatgcg gcggatgacg ggctcataga
     1861 gcttctccca gaagctcctc ggccacatga aggtcttgtt gtagaagccg gcggggaaga
     1921 aggcgcccag cttgtcgttc agcgcgccga agtcgaaatc gaggttgggg aagcggttct
     1981 ggctgcccgc ctccagccct tcatacagcg gcaccgaggg cgcgcgcagg ttgggcgtgc
     2041 gccgggccgg gccgcggttc acgttgacca gcgcgttggg ctcttccacg cccatgccca
     2101 acagcccgcg cgggcggtga tatttgaacg aacggcccag cagcatcacg ccgctggcca
     2161 gcagcgcgga ggcgagattg tcgccggcaa ggcccgtgta ggggcggccg tcgaaggtga
     2221 agggcagcgg ggcggcgcgg ttgacccggc cgcctgcggg cagacgatag ccgctcattt
     2281 gcggggctct ccagccttgt aggtggtttc gaaaaggtcg gtcacggtat cgcgcacggc
     2341 gttgaagaaa cgcccgcaac cgtgggtgtg gcgccagcgc tcatggtgcc ggccgcgcac
     2401 attggcgcgg atgtagagat agctttccca ttccggatcg gtctggccgg aagggtcgag
     2461 cgggcgggcg atatgcgcct cgccgccata gacgaattcc acctcggggc ggtcttcccc
     2521 gcaatagggg caatggatga ggatcatggc ggtttccccc tcagtgagcc acggcggccg
     2581 cggccgcctc gtcgatcagg cgcccgtcgc ggaagcggtc cagcgtgaag ggggcgttga
     2641 tccggtgggg ttggtcatgg gcgatggtgt aggccaagag gtcggcagcg cccggcgtgg
     2701 ccttgaagcc gcccgtgccc cagccgcaat tgacatagag gcccggcacc ggcgtcttgc
     2761 cgaggatcgg cgagcgatct ggcgtgacat caacgatgcc gccccagctg cgcagcatgc
     2821 gcatgcgggt gaagatcggg aagatctcgc agatcgccgc cagcgtgtgc tcgatgatgt
     2881 gcagcgcgcc gcgctgcgaa taggagacat actgatcggt gcccgcgccg atcaccagct
     2941 cgcccttgtc ggactggctg atataggcat gcaccgtgtt cgacatgacc acgcagggga
     3001 agatcggctt caccggctcc gacaccagcg cctgcagcgg ataggattcc agcgggaagc
     3061 gcaggcccgc catgttcatc accaccgagg tgttgccggc agccgacacg cccaccttct
     3121 tggcggcgat aaagcctttt tccgtttcca ccccctgcac cgcgccatcg ggcccgcggc
     3181 ggatgccggt gacggcgcag ttctggatga tatccacccc cagcgccacc gccttgcggg
     3241 catagcccca ggccacgcta tcatgccggg caatgccgcc gcgccgctgc agcgcgccgc
     3301 ccagcaccgg gtggcgggca ttggggttga tatccagcgg cgggcagtat gccttgcact
     3361 cctcgggcga cagccactca ttgtccaccc cgttcagccg gttggcgtgg atgtggcgct
     3421 tgaagctctg gatgtcgtgc acattatgcg ccagcatcag cacgccacgc ttggaataca
     3481 tggtgttgta gttgagctcg tggctcagcc cgtcccacag tttcagcgcg tgatcgtaga
     3541 gatgggcgct ttcatcgtag agatagttgg agcggatgat ggtggtgttg cgcgcggcat
     3601 tgccgccggc cagccagccc ttctcgatca cgcagacatt ggtgatgcca tatttggcag
     3661 ccaggtaata ggcagcaccc agcccatgcc cgccagcacc gacgatgatc acgtcatagg
     3721 cgggtttggg ctccttctcg ggccagtgct cgggccagcc ctgatgcccc ttcagggcct
     3781 cggcaaaaag gttgagcgcg gaaaaacgtt tcatgggtgc tgccttgcct cgtcagctta
     3841 cgggtttggg aggaagatca ggcgccgagg gcggcctggg ccttctccac gtagaaggtc
     3901 ttgaatgcgt cgacgaggtt ctcttccagc gcatacatgc cgggccgata gccatcggtg
     3961 gcaatgccgg cgtggttgag ctcggcgagc ttcctgtcct gctggttggt ggcgttccac
     4021 acggcggtga ggttctccac cgtatagtcc tcgccttcca cggcgtcctc gtgcaccagc
     4081 cagctggtgc gcaccagcgt cttgtcgggc gcgatgggca gcacgctgaa ggtgatcacg
     4141 tgatcgcaac agaagtggtg ccagctgttg gggtgcgtcc agatggagag gctggtcggc
     4201 tcggcgtggt cgacattcca gatcagcttc tgcgaggccg gctggccgtt cagcgtctgg
     4261 ctgagcgaac cattggccag gccaatgcgg gcgatgcggt gccaccagtt gtcggggaat
     4321 tcgaccagat cgtggtgaat gcccagatcc ttccagcggg cgatctcgtc gcccacatcg
     4381 gcatcggcgc cggcggccac gccatcttcc ggattgccct tgccaaaacc gtcacccgag
     4441 agcgagttga gcagttcggg gtgattggag gcgcagtgat agcactcgcg gttgttctcc
     4501 atcaccagct tccagttggc gttgtcgatc agatcgcttt gcgccgccac cttcagcttg
     4561 ccgacattgt agagcccgag ctgggcgccg atatcggcct tcacccggtc gatcggcggg
     4621 ggattgtcgg ccaggcacac aaacagcagc ccgccgacat tctccagatg caccgggcgc
     4681 aggccgtggg cgtccttctc gaagccctcg ggcatgccgc gcgcggcgat cagcttgcca
     4741 tccagcccga aggtccagaa gtgatagggg cacatcaccc ggcccatatg ccccttctgg
     4801 tgttcgcaca gcttggcgcc gcgatgggtg caggtgttgt agaaggcgcg gatctcgcct
     4861 tccttgtcct tcaggatgac gaccgaatta ttggccagct cgaagacgaa gtagtcgccc
     4921 gggttcttca catgggcgat ggtgcaggca tagagccaca ccgacttcag cataacttcc
     4981 gtatcgaagc ggaaggcttc ctcgctgacg tagagctcgc gcggcagggt gtagccgggg
     5041 cggttctggt caagcagggc ggcaatcgag tcgaactcgt tcatggggct taactccgca
     5101 aagcagtgtt ctcgggatca aacggagagt cggcgatcac cacggccttg aagctggtgc
     5161 caaggatggt gatctccagc tcggtgccaa cggcagcatg atccggccga accatggcca
     5221 acgcaagcga cttgccgcag cgccagccat atccgcccga ggtgacgcgc cccaccagtg
     5281 cgccatcctt gaagatcggc tcggacccgc gggcatcagc atctgtgaca tccttcacct
     5341 ccagggtgac gaactgccag gccagccctt tttccttacg ggccagaagc cccgagcgtc
     5401 ctttaaaatc gggcttcttg aaagagatga aacgctcgag cccggattca agcgctgcat
     5461 attcgatcga catctcgcgc gggatgagct tgtagctctt ctcgatcgac atggcgatca
     5521 tcgccttgat gccgaagggg cggatgtcga attcggcacc cgcggccatc agcttgtcaa
     5581 agatgtagtt ctgcatttcg atcggatggt ggatttccca acccagctcg ccgatgaaat
     5641 tgacacgaag ggcttccaca cgggccgcgc caatgctgat cgtcttgccc gtcagccagg
     5701 ggaaggcctc gttggaaagg tcggtgttgg tcagcttggc cagcacatcg cgagacttcg
     5761 gccccgccag caccagcacg cccatcgccg tcgtcagccg ctcatagcgc accgaaccgt
     5821 cggtcggcat ggccttgagc agatagtcgt ggtcgtggcg ctccagcgcg ccggccgaaa
     5881 ccaggtaata ctgctgcggg cccttcttgt agacggtgaa ttccgcccgc acgccgccat
     5941 tgtcggtcag caggtaggag agcgagacgc ggccggtctt cttgggcacg gcattggtca
     6001 gcaggccgtt cagccattcc tcggcgcccg ggccagagat ctggcacttg gcgaaggggc
     6061 tcatgtcctg gatgcccacc ttctcggtca cgtggcgcac ttcggcacca acgaaatcga
     6121 agtagttcga gcggcggaac gaccacttct cgcggatcgt ctcgcccggc accaccgggt
     6181 ggttctcgtt caggatgaca tcggccttgt cgagatcggc ctcgctcagc tcgtagccct
     6241 ccggcgcgaa ccagcccggg cgctcccagc catagacgct gccgaacacc gcgcccagcg
     6301 ccttcatccg gtcgtggcag ggcacgcggc gcagggcgcg ggcggcctcg cgctcttcat
     6361 cgggatagtg gatggcgaag accttggcat aggcctcctc gttcttctcg acgagatagc
     6421 cctcgcccgc atattcgccg aagcggcgcg gatcgacgcc catcatgtcg atcgacggct
     6481 caccgttgac gatccattcg gccagctgcc agccagcgcc gccggccgcc gtgatgccga
     6541 aggaatggcc ctcgttgagc cagaagttct tcttgtccca ggccgggccg acgatgggcg
     6601 agccatcggg cgtgtaggcg atggcgccgt tgtagacctt cttcacgccc acctcgccaa
     6661 aggcgggcac gcgctcgatc gccgagagga tgtagggctc cagccgctcc agcgcatcgg
     6721 ggaacagctc atattcgctg ttcgccgccg ggcccttcac atagcaggcc ggcgcgccga
     6781 tttcgtaggg cccgagcagc aggccgccgg cttcctcgcg catgtaccag ctggcatccg
     6841 attcgcgcag aacgcccatt tccggcaggc ccttggcctt gcgctccatg atcgccgggt
     6901 gctgctcggt gacgatatac tggtgctcca ccggaacaac cgggatgtcg aggccaacca
     6961 tcgcgccggt ctggcgggca aagctgccgg tggccgaaac gacatgctcg caggtgatct
     7021 cgccctggtc ggtcgtcacc acccattcgc cattggcctt ctgggtgatg cccagcacgg
     7081 tggtgttgcg atagatggtg gcgccgcgct ggcgggcgcc cttggccagc gcct
//
LOCUS       DASRYE010000287         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_141293,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000287 DASRYE010000000
VERSION     DASRYE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141293"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFF98_14660"
     CDS             <1..245
                     /locus_tag="VFF98_14660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZU64924.1"
                     /translation="LAVAMWVGRFFFMLPVLAIAGSLAAKKITPETAGSFPTTGALWI
                     GLLVAVIVIVGGLTFLPALALGPVADHLAMIAGQTF"
     gene            261..2288
                     /gene="kdpB"
                     /locus_tag="VFF98_14665"
     CDS             261..2288
                     /gene="kdpB"
                     /locus_tag="VFF98_14665"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZU64925.1"
                     /translation="MSDHTHSAPKSMFSADLVLPAIPEAFRKLAPRALVKNPVMFTTA
                     VVALLLTLLLVIGGSGLAIGFQLQLIVWLWLTVLFGTFAEALAEGRGRAQAASLRATR
                     QELTARLPDGRLVPASQLKKGDTAVVQTGDLIPADGEVIAGVASVNEAAITGESAPVI
                     REAGGDRSAVTAGTRVISDSITIRITQEPGQGFLDRMIALVEGAQRQKTPNEIALTIL
                     LVGLTIIFLIAVATIPAFAAYAGGSIPVAVLAALLITLIPTTIAALLSAIGIAGMDRL
                     VRFNVLAKSGRAVEAAGDVDVLLLDKTGTITVGDRAASEFRPLSGVIEADLAGAALLA
                     SLADETPEGRSIVALAREAHGLAASLPAGAQVIPFTAQTRISGVDLGERLIQKGAVDS
                     ILKANPRLAESAAATELRRHAEEIGRAGQTPLAVAENGRLLGVIALKDVIKAGVRERF
                     AELRRMGIRTVMITGDNPLTAAAIAAEAGVDDFLAEATPEDKLALIRKEQQGGRLVAM
                     CGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSDPTKLIEIVGLGKQLLMTRG
                     ALTTFSVANDVAKYFAIIPAMFVVLYPGLSALNIMHLANPRSAILSAIIFNALIIPAL
                     VPLALKGVRYRPIGAGPLLARNLAIYGAGGLVAPFIGIKAVDIAVSALGLA"
     gene            2345..>2807
                     /locus_tag="VFF98_14670"
     CDS             2345..>2807
                     /locus_tag="VFF98_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HZU64926.1"
                     /translation="MLTDLTSSLRPAVVMTGLFFALTGLAYPLAITGVAQALFPAAAN
                     GSLVYRQGAPVGSALIAQGFARADYFHPRPSAAGKGYDATASSGTNYAPTAKALADRV
                     RADVAANPGAPADMLTTSASGLDPHISPEAALWQAPQVAAARHMPVTLVNRL"
BASE COUNT          407 a          968 c          958 g          474 t
ORIGIN      
        1 cgctggcagt ggcgatgtgg gtgggccgct tcttcttcat gctgcccgtg ctggcgattg
       61 ccggcagcct tgcggccaag aagatcacgc cggaaaccgc cggcagcttc cccaccactg
      121 gcgcgctgtg gattggcctg ctggtggcgg tgatcgtcat cgtgggcggg ctcactttcc
      181 tgccggcgct ggcgctgggg ccggtggccg atcatctggc gatgatcgcc gggcaaacct
      241 tctgaaaaag ggcatgaacc atgtctgatc atacgcattc tgcccccaaa tcgatgtttt
      301 cggcagatct ggtgctgccg gccatccccg aggccttccg caagctggcg ccgcgcgcgc
      361 tggtgaagaa cccggtgatg ttcaccaccg cggtggtggc cctgctgctg accctgctgc
      421 tggtgatcgg cggcagtggc ctggccatcg gctttcagct gcagctgatc gtctggctgt
      481 ggctgacggt attgttcggc acctttgccg aggcgctggc cgaggggcgc gggcgggcgc
      541 aggccgcctc cttgcgcgcc acccggcagg agctgaccgc ccgcctgccc gatggccggc
      601 tggtgcccgc cagccagctg aagaagggcg ataccgccgt ggtgcagacc ggcgacctga
      661 tccccgccga tggcgaggtg atcgccggcg tcgcctctgt gaacgaggcc gcgatcaccg
      721 gcgagagcgc gccggtgatc cgcgaggcgg gcggcgatcg cagcgcggtc accgccggca
      781 cccgcgtcat ttcagactcg atcaccatcc gcatcacgca ggagcccggt cagggtttcc
      841 tcgaccggat gatcgccctg gtggaaggcg cccagcgcca gaaaaccccc aacgagatcg
      901 ccctgacgat cctgctggtg ggcctcacca tcatcttcct catcgctgtg gccaccattc
      961 cggcctttgc cgcctatgcc ggcggcagca ttccggtggc ggtgctggcc gcgctgctga
     1021 tcacgctgat ccccaccacc attgccgcgc tgctgtcggc catcggcatt gccgggatgg
     1081 acaggctggt gcgcttcaac gtgctggcca aatcgggccg cgcggtggaa gcggcgggcg
     1141 atgtcgatgt gctgctgctc gacaagaccg gcacgatcac cgtgggcgac cgcgccgcca
     1201 gcgagttccg cccgctttca ggcgtaatcg aggcggatct ggcgggcgct gcgctgctgg
     1261 cctcgctggc cgatgaaacg cccgaggggc gcagcatcgt tgctctggcg cgtgaggcac
     1321 atggccttgc cgccagcctg ccggccggcg cgcaggtgat ccccttcacc gcgcaaacgc
     1381 gcatctccgg cgtcgatctg ggcgagcggc tgatccagaa gggggcggtc gattccatcc
     1441 tcaaggccaa cccgcgcctc gccgaaagcg ccgctgccac cgaactgcgc cgccatgccg
     1501 aggagatcgg ccgggccggg cagaccccgc tggcggtggc cgaaaacggc cggctgctgg
     1561 gcgttatcgc gctgaaagac gtgatcaagg ccggcgttcg cgagcgtttt gccgaactgc
     1621 gccggatggg catccgcacg gtgatgatca ccggcgacaa tccgctgacc gccgccgcca
     1681 tcgccgccga ggccggggtg gacgatttcc ttgccgaggc cacgccggaa gacaagctgg
     1741 ccctcatccg caaggagcag cagggcggcc ggctggtggc gatgtgcggc gatggcacca
     1801 atgatgcgcc cgcgctcgcc caggccgatg tgggcgtggc gatgaacacc ggcacccagg
     1861 ccgcgcgcga ggcgggcaat atggtggatc tcgacagcga tccgaccaag ctgatcgaga
     1921 tcgtgggcct gggcaagcag ctgctgatga cgcgcggcgc cctcaccacc ttttcggtgg
     1981 cgaacgacgt ggccaaatat ttcgccatca tcccggcgat gttcgtggtg ctctatcccg
     2041 ggctgtcggc gctcaacatc atgcatctgg ccaatccgcg cagcgccatc ctctcggcga
     2101 tcatcttcaa tgcgctgatc atcccggcgc tggtgccgct ggcgctgaag ggggtgcgct
     2161 atcggccgat cggggcgggg ccgctgctgg cgcgcaatct ggcgatctat ggcgcgggcg
     2221 ggctggtggc gccgttcatc ggcatcaagg cggtggatat cgccgtctcg gcgctgggcc
     2281 tggcctgaac aagcttggcc tgaaagagcc cagcccgcac gcattttgtc tggaaggacg
     2341 acccatgttg acagacctca cttcttccct gcgcccggcc gtcgtgatga ccggcctgtt
     2401 cttcgcactc accgggcttg cctatccgct ggcgatcacc ggcgtggcgc aggcgctgtt
     2461 cccggccgcg gccaatggca gcctggtcta ccggcagggc gcgccggtgg gctcggcgct
     2521 gatcgcgcag ggctttgccc gggcggatta cttccacccg cgcccctcgg cggccggcaa
     2581 gggctatgat gccaccgcct cttccggcac caattacgcc cccaccgcca aggcgctggc
     2641 cgatcgggtg agggccgatg tcgccgccaa ccccggcgcc ccggccgata tgctgacaac
     2701 ctcggcctcg ggccttgatc cgcatatctc gcccgaggcc gccctgtggc aggcgccgca
     2761 ggtggcagcc gcgcgccaca tgcccgtcac gctggtgaac cggctgg
//
LOCUS       DASRYE010000288         8582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_104724,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000288 DASRYE010000000
VERSION     DASRYE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104724"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFF98_14675"
     CDS             complement(<1..462)
                     /locus_tag="VFF98_14675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64927.1"
                     /translation="MKTTARVVVIGGGVVGVSTLYHLAKLGWSDVALIERKELTSGST
                     WHAAGLLPLFNLSYSVGKIHQYSVNLYPSLEEETGLNVGFSNVTNIRLATSKDRWDEY
                     LYYAGVARTIGVDVKLLSADEVRDVWPLAQTDGIIGAIQHPDDGYIQPADLT"
     gene            735..2051
                     /gene="glyA"
                     /locus_tag="VFF98_14680"
     CDS             735..2051
                     /gene="glyA"
                     /locus_tag="VFF98_14680"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZU64928.1"
                     /translation="MTQTSTLEPSAARPDTAPFFGQHLADSDPALSAALDAELLRQQD
                     QIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCEKVDIAEELAIARAKELF
                     GCAFANVQPHSGAQANQAVLLALAKPGDTVLGMSLAAGGHLTHGASVNLSGKWFNAVG
                     YGVDPVTHQIDYDQVAELAREYHPRIIIAGGSAYPRIIDFARMRAIADEVDAYLWVDM
                     AHFAGLVAGGAYPSPLPHAHVVTTTTHKTLRGPRGGMILSNDEALGKKINSAVFPGLQ
                     GGPLMHVIAAKAVAFGEALRPAFADYAAAMVANARLLAEGLVARGFVITSGGTDSHLM
                     LVDLTPKGLTGRDAEHALDRAGITCNKNGIPFDPLKPTVTSGIRLGTPAGTTRGFGPE
                     EWRQIGTLIGDVLDGLAANGAEGNGAVEAAARLATAKLCADFPIYP"
     gene            2108..2512
                     /locus_tag="VFF98_14685"
     CDS             2108..2512
                     /locus_tag="VFF98_14685"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HZU64929.1"
                     /translation="MHAWRAFLAGHRRWAFLLLALALAARAMVPAGFMPAAGAHGVAI
                     EICSGSGGPSLFLALPGKTQGPEEGSDSHGGAPCAFSALADTGLQDGPVLALAAPALP
                     PASSHPPLPAAGPSPAAAPYLLPPLRGPPALA"
     gene            2584..3579
                     /locus_tag="VFF98_14690"
     CDS             2584..3579
                     /locus_tag="VFF98_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64930.1"
                     /translation="MTLSTRARRAALLTPLALLAAPAPAMACACGCGIFDVGDGTLMA
                     DADSGLTIWARYAHMVQDQLREGDHRANPADNGDKRIATDFYTIGADYRINARWQVKL
                     ELPLVQRAFTTTDDGTYAAAEGTVNTRRLTDLGDMTLRATYTGLFANGATGLTLGLKL
                     PTGRASSPAGPLGGAGFDRDTLPGTGSTDLILGAHHVGMITPRLSYFVQGQYQFAMST
                     RDGYRPGNEADATVGLTYDTSASPDGFGILPGIQLIGATRAHDTGDNADPLNTGYRRL
                     LIAPGVKWRLSHRASLFTDVAFPIAQHVNAGDPALGDSGQLVAPAQLRVQFTYKI"
     gene            complement(3668..8572)
                     /locus_tag="VFF98_14695"
     CDS             complement(3668..8572)
                     /locus_tag="VFF98_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64931.1"
                     /translation="MTGGAGATGISISGNAGSVQVTTMVDSANSKAAAITVQDNGSSG
                     ALGIAIAGTTGATTENVQLGDALSVTSTGGGASGITINGGTGQGVTLAKGLTVSAGGG
                     NAYGVTLGKNTAGTGALSLTDTGTFQVSNSGSNGIATGIYARGGTTQTLQLNNGLSVS
                     ASTSATGFNGGNASGAVQLTSVSDYTVSGGTGDSFGVNLSGNSGDQTLTFDTAFKTTA
                     TEGAATGISVSNGNSLTVNANAGFTVTSSSDSVNNTVGLAATNVGSVQAKLKGAVDVE
                     GGFAAGFDVEGTNGTASVEIDGPLTVKGSQWAMGGYVYSTSSTSNLSVVLGSDTNITG
                     GADGALGVVLISPATLSFTQSGAFNVSSTSSSIGLELADIGNRTVTFNGPVSVTGGDS
                     RLGGGVYGIYDNAGISTSTATNLTVNSTFAVTNNGTSNVYGLWGQSGVYTLAFNDGLT
                     VSGNGGGAYGIYLSGLGYTGEGAGSTITITGPLSVTQNGEVAGATATGILSTLLGGGM
                     TVSVSGALTASSMGAATGISLSSSSGDLAITTGGAVNVTSYADAATGLAASASAGNVS
                     ITADKAVTVTANGGSALGVSGASSSGYANAITLDAGLTVTNSNGGATGIAATDGTQTI
                     TLTGPVSVTASGGDAYGINSSNSGGATTIHAPSSISATSTTGIAYGIYIDPPASLTID
                     QGGTITASGVGSTYGLYSTGVTGAQTVTLGNVTATSSEADATGISLNGSGAITLTDTG
                     TIAANGVSNGSGVTLATTAAAANITATLGTVTTTGSTAPGVSLAQANGDGTSGAITAT
                     VGSVSTSGNGSTGVVLNGSDTGAVSLTIGQSVEGGHSGGISTTGSGADGVALTTVDGA
                     GTVTNYGTISTAGTGGSDGIYAKSTGAGAITVNSYKVTTSAAGSDAIYAQSVSGAVTI
                     GSTSVSTTKDGSRGIVANSTSGNVAITSGTISTSGTGSDGISASTGGTVNITTTGTTT
                     TTGAGATGLYAFSNSGAITLTTAAISATGNGALGIRAISQSGAITINATSTIANVPAI
                     LANSNTGNITLNLADGGTTSSQVGPAVDLYSGSGTVTINLGSLRTTALVSGDYAGIYS
                     YAGGGQVLNLSGTVEANSGEALWLGGGAAAINNAGTLNGYIDASEAGITTFTNTGTWN
                     AYAADSAFAAGSTIANSGTVNIFPTGFVATGVASPALAGGQTMNITGLASFANSGTLS
                     LANGQAGDVLNLGTASFIGSGHSTLVLDANLAGVAVGTSAAQTADRLVVGASSGVTTI
                     SIKDLGAGMAGQFNFNGIRVVTNSSSTAGAYVLAGGSINKGFVQYQLLQDTNANWDLV
                     GVPSAQAFELVRTVAEAQKFWRLSGDAWAQQMQTKQTREGWQGWVQAYGGTETDRSKP
                     VYKETVLGQQVAFTPNLDIRNDAAGVQLGFEWGHAGLGLGITGGYGTQTGHVEATGDQ
                     IKIDGGNVGLYARYVSPEGLFAHMLGKVDRYTVKYALGSASAPSFGGTTYGVELEAGY
                     HLRSGSLFVEPVLGISWTHSDLDGFSGGSGGMSVAFSHTTSAYGHAGLRAGIETTSGN
                     WKLRPYLGAGWEGEMGDAAHALLTSGGTGLGFDDLKDKGRARLEGGIEGTSAKGFGIF
                     ARVQGVTGSRSQGIGGQAGVTFHF"
BASE COUNT         1350 a         2932 c         2853 g         1447 t
ORIGIN      
        1 ggtcaggtcg gccggctgga tatagccatc gtcggggtgc tggatggcgc cgatgatgcc
       61 gtcggtctgg gccagcggcc acacgtcgcg cacttcatcg gccgacagca gcttcacatc
      121 gacgccgatg gtgcgcgcca cgccggcata atagagatat tcgtcccagc ggtccttcga
      181 ggtggcgaga cggatgttcg tcacgttcga gaagccgaca ttgaggccgg tttcctcttc
      241 caggctggga tagaggttga ccgaatactg gtggatcttg cccaccgaat aggaaaggtt
      301 gaacagcggc agaaggccgg cggcatgcca ggtcgagccg gaggtaagct ccttgcgctc
      361 gatcagcgcc acatccgacc aaccgagctt ggccaggtgg taaagcgtgc tcacgcccac
      421 cacgccgccg ccaatcacca ccacgcgtgc agtcgtcttc atgttcttcc ccggttcacg
      481 gatgcctgtt cgatggtttg actatggcca cagcgcgcag gccggtgcaa cggcggcggc
      541 agccattcga catactgatg gccaagatta gaagaactaa atccaccgcc ccccgcttac
      601 ccccgcgcca ccgccgcgat agcatcctgc ccacaagacc agacacgtgg cccagaagca
      661 ggcccgcccg gggcccggca aaggccggat gcccctgccc aaccggcaga ccaccccaca
      721 ggagcatgac gcgcatgacc cagacctcca ctctcgagcc gtcggccgcc cggcccgaca
      781 cggcgccttt cttcggccag cacctggccg acagcgaccc ggcgctgagc gctgccctcg
      841 atgccgagct gctgcgccag caggaccaga tcgagctgat cgccagcgag aatatcgtca
      901 gccgcgcggt gctggaagcg caaggcagcg tgctgaccaa caaatatgcc gagggctatc
      961 ccggcaagcg ctattatggc ggctgcgaga aggtggatat cgccgaggaa ctggcgattg
     1021 cccgcgccaa ggaactgttt ggctgcgcct ttgccaatgt tcagccgcat tcgggcgcgc
     1081 aggccaacca ggccgtgctg ctggcgctgg ccaagcccgg cgacaccgtg ctgggcatga
     1141 gcctggccgc cggcggccac ctcacccatg gcgcctcggt gaacctgtcg ggcaagtggt
     1201 tcaacgcggt gggctatggc gtcgatccgg tgacgcacca gatcgactat gaccaggtgg
     1261 ccgaactcgc ccgtgaatat catccgcgca tcatcatcgc cggcggcagc gcctatccgc
     1321 gcatcatcga ttttgcccgc atgcgcgcca tcgccgatga ggtggatgcc tatctgtggg
     1381 tggatatggc ccattttgcc ggcctggtgg caggcggcgc ctatcccagc ccgctgcccc
     1441 acgcccatgt ggtgaccacc accacccaca agaccctgcg cggcccccgc ggcggcatga
     1501 tcctttccaa cgacgaggcg ctgggcaaga agatcaattc ggcggtgttc cccggccttc
     1561 agggcgggcc gctgatgcat gtgatcgccg ccaaggcggt ggcctttggc gaagcgctgc
     1621 ggccggcctt tgccgattat gccgccgcca tggtggccaa tgcccgcctg ctggccgaag
     1681 ggctggtggc gcgcggcttt gtcatcacct ctggcggcac cgacagccac ctgatgctgg
     1741 tggacctgac ccccaagggc ctgaccggcc gcgatgccga acatgcgctg gaccgcgccg
     1801 gcatcacctg caacaagaat ggcatcccct tcgatccgct taagcccacg gttacctcgg
     1861 gcattcgcct gggcacgccg gccggcacca cccgcggctt cggcccggaa gaatggcgcc
     1921 agatcggcac gctgatcggc gatgtgctgg atggccttgc cgccaacggc gccgagggca
     1981 atggcgcggt ggaagcggcc gcccgcctgg ccaccgccaa actctgcgcc gatttcccga
     2041 tctacccgta agccccaagc gggccggcgc gcttggcagg cgcgcgccgg cccgctaaga
     2101 ctggcccatg cacgcatggc gcgccttcct tgccggccac aggcgctggg cctttctgct
     2161 gctggcgctg gcgctggcgg cccgggcgat ggtgccggcc ggcttcatgc cggcagccgg
     2221 cgcgcatggc gtggccatcg agatctgctc gggcagcggc gggccaagcc tgttcctggc
     2281 cctgcccggc aagacgcagg ggccagagga aggctccgac agccatggcg gcgcgccttg
     2341 cgccttctcg gcgctggccg acaccgggct gcaggatggc ccggtgctgg ccctggccgc
     2401 gcccgccctg ccgccggcca gcagccaccc gcccctgccc gccgccggcc cgtcgccggc
     2461 cgccgccccc tatctgctgc cgcccttgcg cggccctccg gccctggcct gaaccctccc
     2521 ccgttcatgc cctgaggcag gccgcccatg cgcggcgcct gcccgtttgc cggagttttc
     2581 ctgatgaccc tttccacgcg cgcgcgccgc gctgccctgc tgacacccct ggccctcctg
     2641 gcggcccctg ccccggcgat ggcctgcgcc tgcggctgcg gcatattcga tgtgggcgat
     2701 ggcacgctga tggccgatgc cgacagcggc ctgacgatct gggcgcgcta tgcccatatg
     2761 gtgcaggacc agctgcgcga aggcgaccac cgcgccaacc ccgccgacaa tggcgacaag
     2821 cgcatcgcca ccgatttcta cacgatcggc gccgattacc gcatcaacgc ccgctggcag
     2881 gtgaagctgg aactgccgct ggtgcagcgc gccttcacca ctaccgatga tggcacttat
     2941 gccgccgccg agggcacggt gaacacccgc cgcctgaccg accttggcga catgaccctg
     3001 cgcgccacct acaccggcct gtttgccaat ggcgccaccg gcctcaccct ggggctgaag
     3061 ctgcccaccg gccgcgcttc cagcccggcg ggcccgctgg gcggcgccgg cttcgaccgc
     3121 gacaccctgc ccggcaccgg cagcacggac ctcatcctgg gcgcccatca tgtggggatg
     3181 atcaccccgc gcctcagcta tttcgtgcag gggcaatatc agttcgccat gagcacgcgc
     3241 gatggctatc gccccggcaa cgaggccgat gccaccgtcg gcctcaccta cgacaccagc
     3301 gccagcccgg atggctttgg catcctgccc ggcatccagc tgatcggcgc cacccgcgcg
     3361 catgataccg gcgacaacgc cgacccgctg aacaccggct atcgccgtct gctgatcgcg
     3421 ccgggggtga aatggcgcct ctcgcacagg gccagcctgt tcaccgatgt ggccttcccc
     3481 atcgcccagc atgtgaatgc cggcgatccg gcgctgggcg attccggcca gctggtggcg
     3541 ccggcccaat tgcgcgtgca gttcacctat aagatctgat ttccgcacat gtgaaagggg
     3601 cgcgagggaa ggatcccccg cgcccctttt ctttgcctgc cggcgccttc gtcagaagtg
     3661 gcccgcctca aaagtggaag gtgacgcccg cctggccgcc gatgccctgg ctgcggctgc
     3721 cggtcacacc ctgcacgcgg gcgaagatgc cgaagccctt ggccgaggtg ccctcgatac
     3781 cgccttcgag gcgggcgcgg cccttgtcct tcagatcgtc gaagccaagg ccggtgccgc
     3841 ccgaggtgag cagcgcatgg gccgcatcgc ccatctcgcc ttcccagccg gcgcccagat
     3901 aggggcgcag cttccagttg cccgaggtgg tctcaatgcc ggcgcgcagg cccgcatggc
     3961 cataggcgct ggtggtgtgg ctgaaggcca cgctcatgcc gcccgaaccg cccgagaagc
     4021 catcaaggtc gctatgcgtc cagctgatgc ccagcaccgg ctcgacaaac aggctgcccg
     4081 aacgcaggtg atagcccgct tccagttcca cgccataggt ggtgccgcca aagctgggcg
     4141 cgctggccga gcccagggca tatttcaccg tgtagcgatc aaccttgccc agcatgtggg
     4201 caaacagacc ttccggcgag acatagcggg catagaggcc gacattgccg ccatcgatct
     4261 tgatctggtc gcccgtcgcc tcgacatggc ccgtctgcgt gccatagccg ccggtgatgc
     4321 caaggccaag gccggcatgg ccccattcaa agcccagctg cacgccagcg gcatcgttgc
     4381 ggatatcaag gttgggggtg aaggccacct gctggcccag cacggtttcc ttgtacaccg
     4441 gcttcgaacg gtcggtctcg gtgccgccat aggcctgcac ccagccctgc cagccctcgc
     4501 gggtctgctt ggtctgcatc tgctgggccc aggcatcgcc cgacaggcgc cagaacttct
     4561 gcgcctcggc cacggtgcgg accagttcga aggcctgggc ggaaggcacg cccaccagat
     4621 cccagttggc gttcgtatcc tgcagcaact gatactggac gaagcccttg ttgatgctgc
     4681 cgccggccag cacataggcc cccgcggtcg acgagctgtt ggtgacaacg cggatgccgt
     4741 tgaagttgaa ctggcccgcc atgccggcgc ccagatcctt gatcgagatc gtcgtcacgc
     4801 cggaggaggc gcccaccacc agccggtctg ccgtttgcgc ggcgctggtg ccaacggcaa
     4861 cgccggcaag gttggcatcc agcaccaggg tggaatggcc cgagccgatg aaggaggccg
     4921 tgcccaggtt cagcacatcg cccgcctggc cattggcaag gctgagcgtg ccggagttgg
     4981 caaagctggc aaggccggtg atgttcatcg tctgcccgcc ggccagcgcc ggcgaggcaa
     5041 cgccggtggc cacaaagccg gtggggaaga tgttcaccgt gccgctgttg gcaatggtgg
     5101 agccggcggc aaaggcgctg tcggccgcat aggcgttcca ggtgccggtg ttggtgaagg
     5161 tggtgatgcc ggcttccgaa gcgtcgatat agccgttgag cgtgccggcg ttgttgatcg
     5221 ccgccgcgcc gccgcccagc cacagggcct cgcccgaatt ggcctccacc gtgccggaca
     5281 ggttcagcac ctgcccgccg cccgcatagc tgtagatgcc ggcataatcg ccgctgacca
     5341 gcgcggtggt gcgcaggctg ccgaggttga tggtgaccgt gccgctgccg ctgtacagat
     5401 ccaccgccgg accgacctgc gaggaggtgg tgccgccatc ggccaggttc agcgtgatgt
     5461 tgccggtgtt cgagttggcc aggatcgccg gcacgttggc gatggtgctg gtggcattga
     5521 tggtgatcgc gccggactgg ctgatggcac gaatacccag agcgccgttg ccggtggcac
     5581 tgatcgccgc cgtggtcagg gtgatcgcac cgctgttgct gaaagcataa aggccggtgg
     5641 caccggcgcc ggtggtggtg gtggtgccag tggtggtgat attcaccgtg ccgcccgtgc
     5701 tggccgaaat gccgtcggaa ccagtgccgc tggtgctgat cgtgcccgag gtgatcgcca
     5761 cattgccgct ggtggagttg gcgacgatac cgcgcgaacc atccttggtg gtgctgaccg
     5821 aggtggagcc gatggtgacc gccccgctca cgctttgcgc atagatcgca tcggatccgg
     5881 ccgcgctcgt cgtcacctta tagctgttga cggtgatcgc gccggcgccg gtgctcttgg
     5941 catagatgcc atcggaaccg cccgtgccgg cggtggaaat ggtgccatag ttggtcaccg
     6001 tgccggcgcc atcgaccgtg gtgagagcca cgccatcggc accgctgccg gtggtgctga
     6061 tcccgcccga atgcccgccc tcgacggact gaccgatcgt gaggctgaca gcgccggtgt
     6121 ccgatccgtt gagcaccacg cccgtgctgc cattgccgct ggtgctgaca gaacccaccg
     6181 tggcggtgat ggcgccgctg gtgccatcgc cattggcctg ggccagagag acgcccggcg
     6241 cggtgctgcc ggtggtcgtc accgtgccca gcgtggcggt gatgttcgcc gccgccgccg
     6301 tggtggccag agtgacgcca gagccgttcg agacgccatt ggcggcaatc gtgcccgtgt
     6361 ccgtcagagt gatggcgccg ctgccattga gcgagatgcc ggtggcatca gcctcgctgg
     6421 acgtggcggt gacattgccc agcgttaccg tctgcgcgcc ggtgacgccg gtgctgtaga
     6481 ggccataggt gctgcccacg cccgaagcgg tgatcgtgcc gccctggtcg atcgtcaggc
     6541 tggcgggcgg atcgatgtag atgccatagg caatgccggt ggtgctggtg gcgctgatcg
     6601 aggaaggtgc gtggatggtg gtggcgccgc cgctgttgct ggaattaatg ccataggcat
     6661 cgccgccgct ggccgtcacg ctcaccgggc cggtaagggt gatggtctgc gtgccgtcgg
     6721 tggcggcaat gccggtggcg ccgccgttgc tgttggtcac ggtcaggccc gcatccagcg
     6781 tgatggcatt ggcatagccg ctggacgagg cgcccgaaac acccagcgcc gaaccgccat
     6841 tggccgtcac cgtcactgcc ttgtcggccg tgatcgacac attgccggcg ctggcgctgg
     6901 ccgcaagacc ggtggccgca tcggcatagc tggtgacatt gaccgcgccg ccggtggtga
     6961 tggccagatc gccactgctg ctggaaaggc tgatgccggt ggcagcaccc atcgacgagg
     7021 cagtgagcgc gccgctgacc gagacagtca tcccgccgcc caacagcgtg ctgaggatgc
     7081 cggtggcggt ggccccggcc acctcgccgt tctgcgtgac cgagagcggg ccggtgatgg
     7141 tgatcgtcga gccggcgccc tcgccggtgt agccaaggcc cgacaggtag atgccatagg
     7201 cccccccgcc attgcccgaa accgtgaggc catcgttgaa ggccagcgtg tagaccccgg
     7261 actggcccca caggccatag acattcgacg tgccattgtt ggtcacggca aaggtgctgt
     7321 tgacggtgag gttggtggcg gtggaggtgg aaatgccggc gttgtcatag atgccgtaca
     7381 caccgccgcc gagcctgctg tcgccgcccg tgaccgagac cgggccgttg aaagtgacag
     7441 tgcggttgcc gatgtctgcc agttcaaggc cgatgctgga agaggtcgag ctgacattga
     7501 aggcgccgct ctgcgtgaag ctcagcgtgg cggggctgat gagcacaacg cccagcgcgc
     7561 cgtccgcgcc gccggtgatg ttggtatcgc tgcccagcac aacgctgagg ttgctggtgg
     7621 atgaagtcga gtaaacgtag ccgcccatgg cccattgcga acccttgacg gtgagcgggc
     7681 catcgatctc gacactggcc gtgccgttcg ttccttccac gtcgaaaccg gccgcaaaac
     7741 cgccctcgac atcgacggcg cccttcagct tggcctgcac cgatccgaca ttggtggcag
     7801 caaggcccac cgtattgttc acggaatcgc tgctgctcgt caccgtgaag ccggcattgg
     7861 cattgacggt gagcgaattg ccattggaaa ccgagatgcc ggtggccgcg ccctcagtgg
     7921 cggtggtctt gaaggcggtg tcgaaggtca gcgtctggtc gccgctgttg cccgacaggt
     7981 tgacgccaaa gctatcgccc gtgccgccgc tcacggtgta atcggaaacg ctggtcagct
     8041 gcacggcgcc gctggcattg ccgccgttga aaccggtggc cgaggtgctg gcgctcaccg
     8101 aaaggccgtt gttcagctgc agcgtttggg tggtgccgcc acgggcgtaa atgccggtgg
     8161 caatgccgtt gctgccgctg ttgctcacct ggaaggtgcc ggtgtcggtc aggctcagcg
     8221 cgccggtgcc agccgtgttt ttgcccagcg tcacgccata ggcattgccg ccgccagcgc
     8281 tgacggtgag ccccttggcc agcgtgacac cctggccggt gccgccattg atggtgatgc
     8341 ccgaagcccc gccgcccgtg ctggtgacag acagggcatc gcccagctgc acgttctcgg
     8401 tggtggcgcc ggtggtgccg gcaatggcaa tgcccagcgc gccgctgctg ccattgtcct
     8461 gcacggtgat cgccgcggcc ttgctgttgg cgctgtccac catggtggtc acctgcacgc
     8521 tgccggcatt gccgctgatg ctgatgccgg tggcgcctgc cccgcccgtc accgacaggc
     8581 tg
//
LOCUS       DASRYE010000289        23613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_45551,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000289 DASRYE010000000
VERSION     DASRYE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 23613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23613
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45551"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFF98_14700"
     CDS             <1..865
                     /locus_tag="VFF98_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HZU64932.1"
                     /translation="AHPCSPAALEAALEAAEAGLIAPLLVGPEAKIRALAQQLGKDLS
                     GFTLVDAAHSHDAAAKAVALVREGKASLLMKGSLHTDELMGAVVNAAAGLRTERRISH
                     AYVMDVPGHNRPLIITDAAINIAPTLEEKADIIRNAIGLAHAIGIDEPKVAILAAVET
                     VNPAMKSTLEAAALCKMAERGQITGALLDGPLAFDNAISEAAAREKGIVSPVAGKAEI
                     LVVPDLEAGNMLAKQLTFLGQADAAGVVLGARVPIILTSRADSLRTRLASAALAVLLA
                     DGKAVLPAGEG"
     gene            867..2081
                     /locus_tag="VFF98_14705"
     CDS             867..2081
                     /locus_tag="VFF98_14705"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZU64933.1"
                     /translation="MRALLCLNAGSSSIKFALYTLAPDATEPEGAAHGQIEKIGIAPR
                     LVVSAPDGAPLHAQEWAHGAGLSHEALLGALLDWAMGHLDGHVLIGIGHRVVHGGADF
                     TAPVRVDGDVLGRLAAIGSLAPLHQPHNLAAISALVALRPDLPQVACFDTAFHHTMAP
                     LATRFALPRALHEAGVRRYGFHGLSYEWIAGQLAVIDPALASGRVIAAHLGNGASLCA
                     LRGGISQDTSMGFSALDGLMMGTRCGTLDAGVVLHLLGERGMSPADVTDLLYRHSGLL
                     GVSGLSSDMRALAASDLPEAAEAIALFAHRAAREAAALAVTLGGLDGLVFTAGIGEHD
                     AATRVRICAELGFLGVVIDEAANAAQAPLISAPTSYVRVRVLPTDEERMIARHTLATL
                     QALPDARRATVP"
     gene            2235..4430
                     /locus_tag="VFF98_14710"
     CDS             2235..4430
                     /locus_tag="VFF98_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU64934.1"
                     /translation="MFSLRDIAQPVEPASASDKGEAIYARFEANPDCLLIPVLDDYQR
                     PLGLIERNRFLTRLAGQYGRAVFGSRSVELVMSADPLVVEADVAPGDFAAMALESSTQ
                     RLLQGFIVTDRGRYVGVGAILDLLRAAASERVETARTLTDLVGDLKASALEVERQRRL
                     AETVVEHIPTILAVCSGETGRMIMVNRAAEAVLGLPREMAVGRRCLEIEPQGLGEQIN
                     RLHEELESQPTKTRLDLEFRPEGGQPPRQLRLMRIPLTMPDGERLNVTVGEDVTEEMA
                     ARARIEELAHFDSLTRLPNRARFHEWLNEAVAAADHSARIARPLAAAAQGALLAIDID
                     RFKSVNDTLGHSAGDALLCEVADRLRAVCAELATGDLADGDVDVLPGRLGGDEFAVVL
                     RGHDVAQAAVRLAGRLVDKLAEPYAIGRQAVHLGASIGLAVCPQDAASAEELMRHADI
                     ALYSAKADGKGVWRRFCQSMQSALQNRHDMERDLRRALENGEIGVHYQPQFSLAEQRV
                     TGFEALMRWRHPERGLVPPASFIPVAEDLGLIVPLGEWILREACRQAARLPEDISMAV
                     NISAVQLDHPGFVAAVVQALASAGLPARRLEIEVTESVLMEDKAQVQASIRRLRELGV
                     RLALDDFGTGYASLSYLQRFPFDKIKIDRTFTRGLPDNPAASAIVAAVTALAGQLGMS
                     TTAEGVEDEEQLAAITRLGCTEAQGYLIGMPGAISDSRLDRPGDMAIAV"
     gene            4499..5419
                     /gene="ppk2"
                     /locus_tag="VFF98_14715"
     CDS             4499..5419
                     /gene="ppk2"
                     /locus_tag="VFF98_14715"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZU64935.1"
                     /translation="MTDTPAALTAEDLLQQEIEDSFDEELETELAEARLELLGESLPE
                     HYGFARLDRRMYFRELFRLQHELVRLQDWVQHKKLKVVVIFEGRDAAGKGGVIKRITQ
                     RLNPRVARVVALPAPSERERSQWYFQRYVPHLPAAGEMVLFDRSWYNRSGVERVMGFC
                     TEDEVEEFFRSVPEFERMLVRSGIVLIKYWFSITDAEQQLRFQMRIHDPLKQWKLSPM
                     DVESRRRWEDYTLAKEAMLEATHIPEAPWWVVRADDKRRARLNCIEHLLAQIPYEDVP
                     RDTVVLPPRVHHPDYIRSPIPDDMHVPDLY"
     gene            5502..6251
                     /locus_tag="VFF98_14720"
     CDS             5502..6251
                     /locus_tag="VFF98_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU64936.1"
                     /translation="MPATVLVTGATSGIGLAAARRFAAAGWQVVATGRRADRLEALQA
                     ELGEGLIPLAFDMRDESAIDAALASLPAGLDRLDALVNNAGLALGTAPAQSADLGQWK
                     QMIETNVTGLVTITHKLLARLIAARGIILNLSSVAATYPYAGGNAYGGTKAFVRQFSL
                     GLRSDLHGTGVRVTSIEPGMTETEFTLVRTGGNQAASDTLYGGMQPLTGEDIAETLFW
                     LATQPAHVNVNTIELMPVAQSFAGFQVARAG"
     gene            complement(6276..7643)
                     /locus_tag="VFF98_14725"
     CDS             complement(6276..7643)
                     /locus_tag="VFF98_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZU64937.1"
                     /translation="MKVNAFVAMAGLMLQAPAAHARLLDSFVVMAPEGAEARAVVDDA
                     TCPALTLDGAQVPMSARAAPATLPARASDSGSAASHPSAFPHLLCSAHLPRSAHTASL
                     AGQALPLAPAVVRRIVVVGDTGCRLKAGKIGKDGTRSEDAWQDCNRASAWPFAQVARS
                     AAALHPDLVLHVGDYLYRESACPAHGADCADTPWGYGEDAWRADFLTPARTLLAAAPW
                     VLVRGNHEDCMRAGQGWWRLLDPHALVPGTDCVREAEDFAGNHADPYAVELGDGARII
                     VADFAAIGEKHLQGPELLRYRTDALAIAGLAKPGDVNFVTSHYPFSAVTISGKKGLKI
                     GYPSVDDAFGGQHPPALPHVTAMIAGHVHLLQVVQPRNAPFQLVNGFSGTQEQEPKAP
                     LNLADLEALAAGEQAGIAMTDIATRFGQFGFSTLDRQADGSWLLIARDVNGKKLLRRI
                     IQPRP"
     gene            7844..8434
                     /locus_tag="VFF98_14730"
     CDS             7844..8434
                     /locus_tag="VFF98_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZU64938.1"
                     /translation="MIASSALAAILCDPRQPDTPIVACNAPFLALTGYARAEVLGRNC
                     RFLQGPATQPVARQQLREAIAARRPVMVDILNYRKDGSPFRNAVMVAPIFDAAGELEY
                     FLGSQVALADAVGEVLGDGWQISARAAARRQVDGLSPRQRNILLEMTAGKLNKQIAWE
                     LGLAERTIKMHRAALLRALGVTSSAEAIRLAVEAGF"
     gene            complement(8431..9189)
                     /locus_tag="VFF98_14735"
     CDS             complement(8431..9189)
                     /locus_tag="VFF98_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZU64939.1"
                     /translation="MSSAPLPSPVLFTPEPLPAQAKRRTAKGERTRAAILDAAERLFA
                     ERGYDGVSLRQIIEEAGVQMGQLQYYFPSKEGVFVSAVERRLPEVEALYAGAVADLAR
                     EAAAGQLALRHVVRAVMTVGRAWLAENDIGRHRYLRMLGLSTLSFNQPDYVAHHTRAF
                     APTNAIVMDWLARLYPGAAPSRIKAAYYFIEANLLGMYCNIDVVFARIGEPRNPASVA
                     RMHADLEDFLAGGVAGLLAQEAGLPVQEAGQPAG"
     gene            9345..11054
                     /locus_tag="VFF98_14740"
     CDS             9345..11054
                     /locus_tag="VFF98_14740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZU64940.1"
                     /translation="MALLWALIGSVEARADGVLPAAFAPGSQCHIALVGQSESEALAR
                     PGSGWVCSPRDYDIAAPRVLLRFDLRGVAALPDELVTSLTRFTRMGLTVIGASGRIAH
                     RDITEAAMQPGLQGWTMRTALPVLAGERPAAVVIAVDGITHPRLLSMARLESAGEAAS
                     VDLGQALLVAALCGMLFVPILANIAFYRVLRERFLLWHTLAVALMLLQTLVTSGLINA
                     IARFTVGEISILSGFTLGGGIAAAALFSANLIEEGKLDPLHRRLLAVAGGWIMAGVLV
                     YLWGASLPGIGEALRPYVWTVYYAQYLPVLALFVWTITVALLRGSRAVRFQIVAWAPF
                     MVLGLVRIGSSLGLAPVPMEMASQQHLCITWEVIVTALAVVDRFMTLKRERDDAQAEI
                     RLMATLVDQDPLTGLLNRRALESRFAELQRAGFRAMAVIDLDHFKAINDTHGHAMGDA
                     VLKIVAEVLASDRHTISVRMGGEEFLLLMAGNDIAERAERRRQAISVRVSAKLPGLDR
                     MVTASMGLAIDLPWPMGRTSFPALYEQCDRLLYEAKHGGRNRSSVEQRIMPPTGRLAA
                     AQG"
     gene            11182..13791
                     /gene="mgtA"
                     /locus_tag="VFF98_14745"
     CDS             11182..13791
                     /gene="mgtA"
                     /locus_tag="VFF98_14745"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZU64941.1"
                     /translation="MHGLTTTSTADPSGWNGLIAAPLDEAFGLLASGEEGLSAQAAAA
                     ALARHGPNRIAEEGRLSALRELWRHAKSPLNGLLLALAAVSWALSDLRSAVVIAVMVV
                     LSVGLGFVQEHRSNLAARRLKRMVQVHASLRRPGLAGADAEGFAEVPLEQVVPGDIVR
                     LSAGDLIPADLRLVHAHDLFVNQSSLTGEAMPVEKEAITPAPGTDPLDRPDLCFMGAS
                     VVSGYAVGLVLRTGRHTFFGEIAEKITHTAATSSFEKGVNRFVWLMLRFMLVMAPTVF
                     LINGLTKHNWWEALFFAVAVAVGLAPEMLPMVVTMNLARGAIAMAGKRTIVKRLDAIQ
                     NFGAMDVLCTDKTGTLTQDKVVLRLALDAGGHRSEEVLAHAWLNSHHESGLRNLMDEA
                     ILAHNEVAHHPLLTGRITKLDELPFDFQRRRVSVLLRQEGEAEPLLVCKGAVEEMLAV
                     CTHVLEGGVEGPLDAARLAAVHMVTDRLNRDGLRVLAIARRRFATAPARLTLAEEEGL
                     TLIGFVAFLDPPKESAAQALADLAAAGVAVKILTGDNDLVAAKVCRDVGLGHVRQLLG
                     HEVAAMDDAALAQAAEDVVLFAKLSPAQKARVIAALQARGHVVGFLGDGINDGPALKQ
                     ADVGVSVDTAVDIAKDSAAIILLEKSLGVLVDGVIEGRRVFANIIKYIRMGASSNFGN
                     MFSVLGASILLPFLPMAPLQVLTNNLLYDFSQTAIPTDMVDEEYLAAPRRWEIGNIAR
                     FMLFIGPMSSIFDYATYAAMIFLFHAWNNVPLFQTGWFVESILTQTLIIHIIRTARLP
                     FIESRASWALIGTTLAVCAVGAWLPFSPLAATLGFVPLPAGYWPVVAGFLLAYAVLTH
                     GVKRWFFRRFGDR"
     gene            complement(13857..15368)
                     /locus_tag="VFF98_14750"
     CDS             complement(13857..15368)
                     /locus_tag="VFF98_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HZU64942.1"
                     /translation="MTNRIAHDRLRSKIATAEEAAALISPGDTVGMSGFTGSGYPKEV
                     PLALARRIEEAQAAGDPFRVSVWTGASTGPELDGALAKSNGIEFRLPYNSDPIAREKI
                     NSGQMNYFDMHLSQVAPMAWQGFLGPLDVALVEVSGIRADGTLIPSSSVGNNKTWLDR
                     ASKIILEVNSWQSADLEGMHDIYYGTRLPPNRVPIPLVKPDDRIGSPYLQIDPDKVVA
                     IVQTDTPDRNAPFSPPDPTAFAIAGHLMEFFKHEVKLGRIPANLLPIQSGVGNIANAV
                     LSGLVDSPFENLTSFTEVIQDGMLDLLDCGKLRMASATAFSLSPEAAARLNANMGAYR
                     DKMILRPQEISNHPELVRRLGCIAMNGLIEADIYGNVNSTHVMGSRIQNGIGGSGDFA
                     RNAFISIFMTPSTAKGGKISAIVPQASHVDHISQDVQVIVTEQGLADLRGLSPKQRAE
                     KIIANCAHPNYRPMLEDYYARALKGSYGLQSPSLPAEALSWHQRFIETGSMLV"
     gene            15722..18916
                     /locus_tag="VFF98_14755"
     CDS             15722..18916
                     /locus_tag="VFF98_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZU64943.1"
                     /translation="MFDIVKVALHRPLTFIVMAILIAIMGTLAALDTPVDIFPEIRVP
                     VIAVAWQYTGLNPKDMSDRIVTPYERTLTTTVNNIEHIESQSMQGIGIVKIFFQPGTN
                     ISVANAQVTAVSQTVLKQMPPGITPPLILNYNASTVPILQLALNGKGLSEQQLFDLGQ
                     NQIRPQLVTIPGLAMPYPSGGKQRQIQIDIDPEELQATGLSAQDVGNAIAAQNQITPA
                     GFVKIGDRQYSVQLNNAPNSVEALNALPVKVVNGATITLGQVAHVRDGFPPQQNVVHV
                     DGQRAVLLTVLKNGATSTLAIIDGVKNKLPQLVIGLPDALKVRLVGDQSLFVKAAVQG
                     VLREGAIAAALTSLMILLFLGSWRSTAIIALSIPLAVLAAVCLLNVFGQTLNVMTLGG
                     LALAVGLLVDDATVTIESIVWHLEQGKGLIEAILDGAAQIVTPAFISLLCICIVFVPM
                     FMLPGVAGYLFVPMALSVVFAMVASFVLSRTLVPTLAMYWLKPHTPAEHDEHAAGSPA
                     SRNVFVRFQRGFEARFLVLRGHYVGLLRLALTHRKPFLIGFGAVVVCSFALLPALGSN
                     FFPKVDSGQIMMHVRVPMGARIEDTARRFDLINRAVREIIPQTELANLTDNIGLPNSA
                     LNTIYSNDGTIGPQDGDIMITLAEGHRPTEQVENQLRKELTARFAGTSFAFLPADITS
                     QILNFGSPAAIDIQITGKDPAATRDYAQKLSARLGGIAGLVDKRIQQPLGTPQLNVDV
                     NRDLIGQYGLTERDVTNSLSNVLAGTSQTAPVFYVNPKNSVQYAVVAQTPEYRVSTLD
                     DLANVPVSGTGGVPNQLLGGVARVTRSATSLVISHYNIAPVVDIYGNTYGRDLGAVSG
                     DIQKAIASLAKEQPKGTQVTLRGQYATMNTAFSGLGFGLLGAVVLIYLLIVVNFQSWV
                     DPMVIVSALPAALAGIVWMLFTTGTTLSVPALTGAIMCMGIATANSILVVSFARERLA
                     DLGDATQAALEAGMVRLRPVMMTALAMIIGMAPMALGMGDGGEQNAPLGRAVIGGLLC
                     ATIATLFFVPTVFSLAHRRYGKTTSQPEAVPAHV"
     gene            18909..20123
                     /locus_tag="VFF98_14760"
     CDS             18909..20123
                     /locus_tag="VFF98_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU64944.1"
                     /translation="MSDPVSHSENAEGDLFETAPDVGKLKRLGVAAGVVALVVAGAGV
                     ASRIHATSELRTAASDSALPDVAVIAPTKPGASAGLVLPGTIQAWNSAAINARTNGYV
                     KRWLADIGDQVRAGQPLAVLDAPEVDQQLAQAAADYQTALANQRLAETTSRRWQLMLS
                     QDAVSRQEADEKAGDLAAKSALANSALANVKRLRAMQGFERLSAPFDGMVTSRSAQIG
                     ALVSTTNASAPPLFTVSDVHRMRIYVRVPQSYSAMLHAGLKATLTLPEFPGRQFAAEL
                     TRSAGAVDPQSGAVLVELQSDNPDRALKPGAFSQVRFDTGAVQGNTLELPSSAIIYGA
                     NGPSIAVVDAQGVVSVRQVAIARDEGNTVLVGGALATTDKVIDSPPDAVKSGDKVRVT
                     QTETAGGAAHGK"
     gene            20113..21573
                     /locus_tag="VFF98_14765"
     CDS             20113..21573
                     /locus_tag="VFF98_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU64945.1"
                     /translation="MANKLRKGRAPLGVALLTSALLGGCVGAPAYHPVKVATPAQFSE
                     TPDGWAAAQPADGAARGPWWQVFADPQLDRLEQQAEKASPTLEAALARYDEARATARV
                     ADAARLPEVDAVGSAARDKVSQNRPLTTGREATYNDYRAGGLLTYELDLWGRVRAAAR
                     AGHAEADASGHDLAAARLSLQAAVADAYVRLRGLDAQASLLRRNDEAYSRAYQLTATR
                     HQGGIASIIDVNRASMVLANARAQLAAVEDQRAATEHEIAALVGAVASNFHLAAVPDA
                     LRVPLAPATTPSVMLQRRPDVAAAERRMAAANARVGAARAALFPTITLGLTGGYETTG
                     PSMFSAPNAFWGLGPASVSLPVFDGGKRRAQLRSARAQYDEVAATYRSTVLTAFREVE
                     DQLAASRLLAHEATQQSLAADAAQHTSDLSFTRYREGATDYFEVVTAQTDALNAQRTL
                     LAVQTQRLQAAIALVKALGGDPAPALGGEPAPGKQG"
     gene            complement(21613..22287)
                     /locus_tag="VFF98_14770"
     CDS             complement(21613..22287)
                     /locus_tag="VFF98_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HZU64946.1"
                     /translation="MLIVIPQVLPREEALALGRELAAAAWVDGNVTSGEGAALAKRNR
                     QLPEEGEAAARGRAIVQRALARNPLFLSAALPYQLFPPLFNRYGPGEFFGDHVDNAVR
                     LDRATGRQLRIDLSATLFLSEDYEGGELTIESGFGKVAHKLPAGDMVLYPSSSLHRVT
                     QVTSGERISCFFWLQSLVRDHAGREALFDLDQAIQRLSVDRGGDDSEVLRLTKAYHNL
                     VRMWAL"
     gene            complement(22368..23480)
                     /locus_tag="VFF98_14775"
     CDS             complement(22368..23480)
                     /locus_tag="VFF98_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64947.1"
                     /translation="MAPSFPRLCAIGLATMLATLLAMMALPHRAYIRWQSEQTEAFAR
                     LGWVYERIHDDPTPMDIAFIGTSHTLNGVDGLAVARGLAQAGVRAPDGRCLTVTNLAI
                     PAYGRNMQWAVAREVLRARHPRLLVLEVLENETRTAHPLFYRVAETADVLGAPKVVNG
                     NYMADVIRLPWRQLVLWAQSLAPAQFGLKAAWSAADYDGSTVDNTRIVNVHGQEMTPL
                     RDRVFPASDLRATAAADMAAKRLHILPPAFDRWEYALTRRYLTGMLDLADSTHTPVLL
                     LYLPAFGRPDQPMDRRWYGGRAMVGVNDILRRPELWNDPQHVNAAGAAAISARLGQVL
                     AARLGAKGPHDVAGCAFGYGPRAVLQPWRAPYHPAN"
BASE COUNT         3726 a         7683 c         8287 g         3917 t
ORIGIN      
        1 tgcccacccc tgttcgcccg ccgcgctgga agcggcgctg gaggcggccg aggccgggct
       61 gatcgcgccg ctgctggtgg ggccggaagc gaagatccgc gcccttgccc agcagctggg
      121 caaggatctg tcgggcttca cgctggtgga tgccgcgcac agccatgatg ccgccgccaa
      181 ggccgtggcg ctggtgcgcg agggcaaggc cagcctcttg atgaagggct cgctccacac
      241 cgatgagctg atgggcgcgg tggtgaatgc cgcggccggc ctgcgcaccg agcggcgcat
      301 cagccatgcc tatgtgatgg atgtgcccgg ccacaaccgc ccgctgatca tcaccgatgc
      361 ggcgatcaac atcgccccca cgctggagga gaaggccgat atcatccgca atgccatcgg
      421 ccttgcccat gccatcggca tagacgagcc caaggtggcc attctggcgg cggtggaaac
      481 ggtgaacccg gcgatgaaat cgacgctgga ggcggccgcc ctgtgcaaga tggccgagcg
      541 cgggcagatc accggcgccc tgctggatgg cccgctggcc ttcgacaatg ccatcagcga
      601 ggcagccgcc agggagaagg gcattgtctc gccggtggcg ggcaaggcgg agatcctcgt
      661 cgtgcccgat ctggaagcgg gcaacatgct ggccaagcag ctcaccttcc tgggccaggc
      721 ggatgcggcc ggcgtggtgc tgggggccag ggtgccgatc atcctcacca gccgggccga
      781 cagtttgcgc acgcggcttg cctcggcggc gctggcggtg ctgctggccg acggcaaggc
      841 ggtgttgccg gccggggagg gctgagatgc gcgcgctgct gtgcctgaac gccggctcct
      901 ccagcatcaa attcgcgctc tacacgctgg cgcccgatgc gacagagccg gaaggcgccg
      961 cccatggcca gatcgagaag atcggcatcg ccccgcggct ggtcgtctcg gcgcccgatg
     1021 gcgcgcccct gcacgcgcag gaatgggcgc atggcgccgg gctttcgcat gaagcgctgc
     1081 tgggcgcgct gctggattgg gcgatggggc atctggatgg ccatgtgctg atcggcatcg
     1141 gccaccgggt ggtgcatggg ggtgcggatt tcacagcgcc cgtgcgggtg gatggcgatg
     1201 tgctgggccg cttggccgcc atcggcagcc tggcgccgct gcatcagccg cataatctgg
     1261 cggcgatctc ggcgctggtg gcgctgcggc ccgatctgcc gcaggtggcc tgtttcgaca
     1321 ccgcctttca ccacacgatg gcgccgctgg ccacccgctt tgccctgccg cgcgcgctgc
     1381 atgaggcggg cgtgcggcgc tatggtttcc atggcctgtc ttacgagtgg atcgccgggc
     1441 agctggcggt gatcgatccg gcgctggcat cgggccgggt gatcgcggcg catctgggca
     1501 atggcgccag cctgtgcgcg ctgcgcgggg gcatcagtca ggataccagc atgggctttt
     1561 ccgcgctcga cgggctgatg atgggcacgc gctgcggcac gctggatgca ggcgttgtgc
     1621 tgcacctgct gggcgagcgc ggcatgagcc cggccgatgt gaccgacctg ctctatcgcc
     1681 actcggggct gctgggggtt tcgggcctgt cgagcgacat gcgcgcgctg gcggccagcg
     1741 atctgcccga ggcggccgag gcgatcgccc tgttcgccca ccgcgccgcg cgcgaggcag
     1801 cggcgctggc ggttacgctg ggcgggctgg atggcctggt gttcaccgcc ggcattggcg
     1861 agcatgatgc cgccaccagg gtgcgcatct gcgccgagct gggctttctg ggcgtggtga
     1921 tcgacgaggc ggccaatgcc gcccaagcgc cgctgatcag cgcgccgaca agctatgtgc
     1981 gcgtgcgcgt gctgcccacc gatgaggaac ggatgatcgc gcggcacacg ctggccacct
     2041 tgcaagccct gccagacgcg cgccgggcaa cggttcctta ggactttacc ggatacaccc
     2101 tgccatgcct cggggccgtt cgttcgcgga tgcagcaacc gggtcgggca cttgctgccc
     2161 gaaggatcgg ggaaagccgc aagggcaaag ccatttgcct gcgccggcgc gtcaaggtgc
     2221 caaaacggta ttgcatgttc agcctgcgcg atattgccca accggtggaa ccagccagcg
     2281 ccagtgacaa gggcgaggcg atctacgccc gtttcgaggc caaccctgat tgcctgctga
     2341 tcccggtgct ggatgattac cagcgcccgc tggggctgat cgagcgcaac cgcttcctca
     2401 cccgtctggc ggggcaatat ggccgggcgg tgttcggcag ccgctcggtg gaactggtga
     2461 tgtcggccga tccgctggtg gtggaagccg atgtggcgcc cggcgatttt gccgccatgg
     2521 cgctggaatc ctccacccag cggctgttgc agggcttcat cgttaccgat cgggggcgtt
     2581 atgtcggcgt gggcgcgatc ctcgatctgc tgcgcgcggc cgcttccgaa cgggtggaaa
     2641 ccgcccgcac gctgaccgat ctggtcggcg atctcaaggc ctcggcgctg gaggtggagc
     2701 gccagcggcg cctggccgaa acggtggtgg agcatattcc caccatcctg gccgtctgtt
     2761 cgggcgagac ggggcggatg atcatggtga accgcgcggc cgaggcggtg ctgggcctgc
     2821 cgcgcgaaat ggcggtgggc cggcgctgcc tggagatcga gccgcagggc ctgggcgagc
     2881 agatcaaccg tctgcatgag gaactggaaa gccagccgac caagacccgc ctcgacctcg
     2941 aatttcggcc cgagggcggc caaccgcccc gccagctgcg gctgatgcgc attccgctga
     3001 cgatgcccga tggcgagcgg ctgaatgtga cggtgggcga ggatgtgacc gaggaaatgg
     3061 cggcacgggc gcggatcgag gaactggccc atttcgattc gctcacccgc ctgcccaacc
     3121 gcgcccgctt ccacgaatgg ctgaacgagg ccgtggccgc ggccgatcac agcgcccgca
     3181 tcgcccggcc gctggccgcc gccgcgcaag gcgcgctgct ggcgatcgac atcgaccgct
     3241 tcaagagtgt gaacgacacg ctgggccaca gcgcgggcga tgcgctgctg tgcgaagtgg
     3301 ccgacaggct gcgcgcggtt tgtgccgagc tggccacagg cgatctggcc gatggcgatg
     3361 ttgacgtgct gcccgggcgg ctgggcggcg atgaatttgc cgtggtgctg cgcggccatg
     3421 atgtggcgca ggccgcggtg cgcctggccg ggcggctggt ggacaagctg gcagagccct
     3481 atgccattgg ccggcaggcg gtgcatctgg gggccagcat tggccttgcc gtgtgcccgc
     3541 aggatgccgc cagcgccgaa gagctgatgc gccacgccga tatcgcgctc tacagcgcca
     3601 aagccgatgg caagggggtg tggcggcgct tctgccaatc catgcaatcg gccttgcaaa
     3661 accgccacga catggagcgt gacctgcgcc gggcgctgga aaacggcgag atcggcgtgc
     3721 attaccagcc gcagttcagc ctggccgagc agcgcgtgac cggctttgag gcgctgatgc
     3781 gctggcgcca tccggaacgc gggctggtgc cgccggccag cttcatcccg gtggccgagg
     3841 atctggggct gatcgtgccg ctgggcgaat ggatcctgcg tgaggcctgc cgccaggccg
     3901 cccgcctgcc cgaggatatc tcgatggcgg tgaacatctc ggcggtgcag ctggatcatc
     3961 cgggctttgt cgccgctgtg gtgcaggcgc tggcgagcgc cggcctgccc gcccgccgcc
     4021 tcgaaatcga ggtgaccgaa agcgtgctga tggaagacaa ggcgcaggtg caggccagca
     4081 tccgccgcct gcgcgagctg ggcgtgcgcc tggcgctcga cgatttcggc accggctatg
     4141 cctcgctgtc ctatctgcag cgcttcccct tcgacaagat caagatcgac cgcaccttca
     4201 ctcgcggcct gcccgacaac cccgccgcca gcgccattgt ggcagcggtg acggcgctgg
     4261 ccggccagct gggcatgtcg accaccgccg aaggggtgga ggatgaggag cagctggccg
     4321 ccatcacccg gctgggctgc accgaggcgc agggctatct gatcggcatg cccggcgcca
     4381 tcagtgacag ccggctggac cggcccggcg atatggccat cgccgtctag cgtcggcttt
     4441 tgtcacacgg ctttcatcca gcgggcataa cccggccctg ccaatggaga aagcccggat
     4501 gaccgacacc cctgccgccc ttaccgccga ggacctgctg cagcaggaaa tcgaggacag
     4561 tttcgacgag gaactggaaa ccgagctggc cgaggcgcgg ctggaactgc tgggcgaaag
     4621 cctgccagag cactatggct ttgcccggct cgaccgccgc atgtatttcc gcgagctgtt
     4681 ccgcctgcag cacgaactgg tgcgcctgca ggattgggtg cagcacaaga agctgaaagt
     4741 ggtggtgatc ttcgaggggc gcgatgccgc cggcaagggc ggcgtgatca agcgcatcac
     4801 ccagcggctg aacccgcgcg tggcccgcgt ggtggcgctg ccggcccctt ccgagcggga
     4861 acgcagccag tggtatttcc agcgctatgt gccgcatctg ccggccgccg gcgaaatggt
     4921 gctgttcgat cgcagctggt acaatcgctc gggcgtggaa cgggtgatgg gcttttgcac
     4981 cgaggacgag gtggaggaat tcttccgctc cgtgccggaa ttcgagcgga tgctggtgcg
     5041 ctcgggcatc gtcctcatca aatactggtt ctcgatcacc gatgccgagc agcagctgcg
     5101 cttccagatg cgcatccacg atccgctgaa gcagtggaag ctgtcgccca tggatgtgga
     5161 aagccgccgc cgctgggagg attacaccct ggccaaggaa gccatgctgg aggccaccca
     5221 tatccccgag gcgccgtggt gggtggtgcg cgccgacgac aagcgccgcg cccggctcaa
     5281 ttgcatcgag catctgctgg cccagatccc ctatgaggat gtgccgcgcg acacggtggt
     5341 gctgccgccg cgcgtgcatc accccgatta tatccgcagc cccatccccg atgacatgca
     5401 tgtgccggat ctctactgat ctactgggat aaagagggct tggcggcagc agggcagggc
     5461 ggctaagagg gggcaaccgc ttcttgccaa aggattatgc catgcctgct acagttctcg
     5521 tcaccggggc cacttcgggc attggcctgg ccgcggcgcg ccgctttgcc gcggcgggct
     5581 ggcaggtggt ggccaccggc cgccgggccg accggttgga ggcgctgcag gcggaactgg
     5641 gcgaggggct gatcccgctg gccttcgaca tgcgcgatga aagcgccatc gatgccgccc
     5701 ttgccagcct gccggcaggg ctcgacaggc tggacgcgct ggttaacaat gccggccttg
     5761 cgctgggcac ggcgcccgct caatcggcgg atctgggcca gtggaagcag atgatcgaga
     5821 ccaatgtcac cggtcttgtc accatcaccc acaagctgct ggcgcggctg atcgcggcgc
     5881 gcggcatcat tctcaacctg tcgtcggtgg cggccaccta tccctatgcg ggcggcaatg
     5941 cctatggcgg caccaaggcc tttgtgcgcc agttcagcct gggcctgcgc agcgatctgc
     6001 acggcacggg cgtgcgcgtc acctcgatcg agccggggat gaccgagacg gaattcaccc
     6061 ttgtgcgcac cggcggcaat caggcggcct ccgacacgct gtatggcggc atgcagccgc
     6121 tgaccggcga ggatattgcc gaaacgctgt tctggctggc aacgcagccc gcccatgtca
     6181 acgtcaacac catcgagctg atgcccgtgg cgcaaagctt cgccggcttc caggtggcgc
     6241 gcgcgggcta ggggccggcg cgcgcccgtt ccccctcagg ggcgcggctg gatgatgcgg
     6301 cgcagcagct tcttgccgtt cacatcgcgg gcgatcagca gccagctgcc atcggcctgc
     6361 cggtccagcg tggaaaagcc gaactggcca aagcgggtgg cgatgtcggt catggcgatg
     6421 ccggcctgct caccggcggc cagggcttcg agatcggcca ggttgagcgg ggccttgggt
     6481 tcctgctcct gcgtgccgga aaagccgttg accagctgga agggcgcgtt gcggggctgc
     6541 accacctgca gcaggtgcac atggccggcg atcatcgcgg tcacatgcgg cagggcgggc
     6601 gggtgctggc caccgaaggc atcatccacc gagggatagc cgatcttcag ccccttcttg
     6661 cccgaaatgg tgacggcgct gaagggatag tggctggtga cgaaattgac atcgccgggc
     6721 ttggccagcc ccgcgatcgc cagcgcatcg gtgcgatagc gcagcagttc ggggccttgc
     6781 aggtgcttct cgccaatcgc ggcgaaatcg gccacgatga tgcgcgcgcc atcgcccagt
     6841 tccaccgcat agggatcggc atggttgccg gcaaaatcct cagcctcgcg cacgcaatcc
     6901 gtgccgggca ccagcgcgtg cggatcgagc aggcgccacc agccctggcc ggcgcgcatg
     6961 caatcctcgt ggttgccgcg caccagcacc cagggggcgg cggccagcag cgtgcgcgcg
     7021 ggcgtcagga aatcggcccg ccaggcatcc tcgccatagc cccagggtgt gtcggcgcaa
     7081 tcggcgccat gggccgggca ggcgctttcg cggtagagat aatcgcccac atgcagcacc
     7141 agatcggggt gcagcgcggc ggcagagcgg gccacctggg caaacggcca ggccgaggcg
     7201 cggttgcaat cctgccaggc atcctcgctg cgggtgccat ccttgccgat cttgcccgcc
     7261 ttcagccggc agccggtatc gcccaccacc acgatgcgcc gcaccacggc cggggccagc
     7321 ggcagcgcct ggccggcaag gctggccgta tgggcgctgc ggggcagatg cgcgctgcag
     7381 agcagatgcg ggaaagccga ggggtggctg gcggcggagc cggaatcgct ggcgcgggcg
     7441 ggcagggtgg caggggcggc gcgggcgctc atcggcacct gcgcgccatc gagggtgagc
     7501 gccgggcagg tggcatcatc caccaccgcg cgcgcctcgg cgccttcggg ggccatcacc
     7561 acgaaactgt cgagcaggcg ggcatgggcc gcaggggcct gcagcatcag ccccgccatg
     7621 gccacaaaag catttacctt catcgatttg ccgagcctgc gcatgggtca cctgcctgtt
     7681 ctgagtcggg tttcggctat cgctgcttgg cgacagtgtg atgaaagggg cgcgatgaaa
     7741 acgtaaaatc cgccgggtac agcctgccct tgccggcttg gaggcggggc gggcagttgc
     7801 tacacacaag gccatgtccg tgcccgagcc cccccttgtc ggaatgatcg cgagcagcgc
     7861 gctggcggcc atcctgtgcg atccgcgcca gcccgacacg ccgatcgtgg cctgcaacgc
     7921 gccctttctg gcgctcaccg gctatgcccg cgcagaagtg ctggggcgca actgccgctt
     7981 cctgcagggg ccggccaccc agcctgtcgc ccgccagcaa ttgcgcgagg cgatcgccgc
     8041 gcgccggccg gtgatggtgg atatcctcaa ctatcgcaag gatggcagcc ctttccgcaa
     8101 tgcggtgatg gtggcgccga tcttcgatgc ggcaggagag ctggaatatt tcctcggctc
     8161 gcaagtggcg ctggccgatg cggtgggcga ggtgctgggc gatggctggc agatcagcgc
     8221 ccgcgccgcc gcccgccgcc aggtggatgg gctgagcccg cgccagcgca atatcctgct
     8281 ggagatgacg gcgggcaagc tgaacaagca gatcgcctgg gaactgggcc tggccgagcg
     8341 caccatcaag atgcaccgcg ccgccctgct gcgcgcgctg ggcgttacca gcagcgccga
     8401 ggcgatccgc ctggcggtgg aggccggctt ctagcccgcg ggctggcctg cttcctgcac
     8461 gggaaggccc gcttcctgcg ccagcaggcc ggccacgccg ccagccagga aatcctccag
     8521 atctgcatgc atgcgggcca ctgatgccgg gttgcgcggc tcgccaatgc gggcgaacac
     8581 cacatcgatg ttgcaataca tgcccagcag attggcctcg atgaaataat aggcggcctt
     8641 gatgcgcgag ggggccgcgc ccggatacag ccgcgccagc cagtccatca cgatggcgtt
     8701 ggtgggggca aaggcgcggg tgtggtgggc cacgtaatcg ggctggttga agctgagcgt
     8761 ggagaggccc agcatgcgca gatagcggtg gcggccgatg tcgttctcgg ccagccaggc
     8821 gcggcccacg gtcatcaccg cgcgcaccac atggcgcagg gccagctggc cggccgccgc
     8881 ctcccgcgcc agatcggcca ccgcgccggc atagagcgct tccacctcgg gcagccgccg
     8941 ctccaccgcc gagacgaaca cgccttcctt ggaggggaaa taatattgca gctggcccat
     9001 ctgcacgccc gcctcctcga tgatctggcg cagcgagacg ccatcatagc ccctttcggc
     9061 aaacagccgc tcggccgcat cgagaatggc cgcgcgggtg cgctcgccct tggcggtgcg
     9121 gcgcttggcc tgggcgggca agggctcggg cgtgaacagg acaggcgagg gcaggggggc
     9181 agacgacatg agcgccattg tgtcggctgg gcaggggcgg tgcaagctgg cctttatcgg
     9241 ggtgggcggg caagggtgtg ccgaaaaggg ccggggcggt aataatatct cagcgatttt
     9301 ctaagtaata atccagaatg tttagcggga gactggtttg ggcgatggcg ctgttgtggg
     9361 cgctgatcgg cagcgtggaa gcgcgggccg atggcgtgct gcccgcagct ttcgcgcccg
     9421 gcagccagtg ccacatcgcc cttgtcggcc agagcgagag cgaggcgctg gcgcggccgg
     9481 gcagcggctg ggtgtgcagc ccgcgcgatt acgacattgc cgccccgcgc gtgctgctgc
     9541 gcttcgacct gcgcggcgtg gcggcgctgc ccgacgagct ggtgaccagc ctcacccgct
     9601 ttacccgcat ggggctgacg gtgatcggcg cctcgggccg gattgcccat cgcgatatca
     9661 ccgaggcggc gatgcagccg ggcctgcagg gctggacgat gcgcaccgcc ctgccggtgc
     9721 tggcgggcga gcggccggca gcggtggtga tcgcggtgga tggcatcacc catccgcggc
     9781 tgctgtcgat ggcccggctg gaaagcgcgg gcgaggcggc cagcgtggat ctgggccagg
     9841 cgctgctggt ggcggcgctg tgcggcatgc tgttcgtgcc cattctcgcc aatatcgcct
     9901 tctaccgcgt gctgcgcgag cgcttcttgc tgtggcacac gctggcggtg gcgctgatgc
     9961 tgctgcaaac gctggtgaca tccgggctga tcaacgccat cgcccgcttc acggtgggcg
    10021 agatttccat cctctcgggt tttaccctgg gcggggggat tgcggcagcg gcgctattct
    10081 cggccaatct gatcgaggag ggcaagctgg atccgctgca ccgccggctg ctggcggtgg
    10141 ccggcgggtg gatcatggcg ggcgtgctgg tctatctgtg gggggccagc ctgccgggca
    10201 ttggcgaggc gctgcggccc tatgtctgga cggtgtatta cgcgcaatat ctgccggtgc
    10261 tggcgctgtt tgtctggacg atcactgtgg cgctgctgcg cggcagccgg gcggtgcgct
    10321 tccagatcgt cgcctgggcg ccgttcatgg tgctggggct ggtgcgcatc ggctcgtcgc
    10381 tggggctggc gccggtgccg atggaaatgg catcgcagca gcatctgtgc atcacctggg
    10441 aagtgatcgt gacggcgctg gcggtggttg accgcttcat gacgctgaag cgcgagcgcg
    10501 acgatgccca ggccgagatc cgcctgatgg ccacgctggt cgatcaggat ccgctgaccg
    10561 ggctgctgaa ccgccgcgcg ctggaaagcc gctttgccga actgcagcgc gccggtttcc
    10621 gcgccatggc ggtgatcgac ctcgaccatt tcaaggcgat caacgacacc cacggccatg
    10681 cgatgggcga tgcggtgctg aagatcgtgg ccgaagtgct ggccagcgac cggcacacca
    10741 tctctgtgcg catgggcggc gaggaattcc tgctgctgat ggccggcaac gatatcgccg
    10801 agcgggccga gcggcgccgg caggcgattt ccgtgcgggt ttcggccaag ctgccggggc
    10861 tcgaccggat ggtgacggcc agcatgggcc tggcgataga cctgccctgg ccgatggggc
    10921 gcacctcctt ccccgcgctc tatgagcaat gcgaccggct gctgtatgag gccaagcatg
    10981 gcgggcgcaa ccgttcctcg gtggaacagc ggatcatgcc gcccaccggc cggcttgccg
    11041 cggcgcaggg ttaggcaatt gcccagggct aggcaattgt ttgtggcgcg ctttacgccg
    11101 gggctatgcc cgggggcggg catgcgctaa cccttgcgcc cggttgcggg gcatctggct
    11161 cctgccaaga ggaccggcac gatgcatggg ctgacgacga cctccaccgc cgacccttcg
    11221 ggctggaacg gcctgatcgc agcgccactg gacgaggcct ttggcctgct ggcctcgggc
    11281 gaggaggggc tctcggcaca ggcggccgct gccgcccttg cccgccacgg ccctaaccgc
    11341 attgccgagg aagggcgcct ttctgccctg cgcgagttgt ggcgccatgc caaaagcccg
    11401 ctcaatggcc tgttgctggc gctggcggcg gtttcctggg cgctgtcgga tctgcgctcg
    11461 gcggtggtga tcgcggtgat ggtggtgctc agcgtggggc tgggctttgt gcaggagcac
    11521 cgctcgaacc tggctgcccg gcggctgaag cgcatggtgc aggtgcatgc cagcctgcgc
    11581 cggcccggcc tggctggcgc cgatgccgaa ggctttgccg aagtgccgct ggagcaggtg
    11641 gtgccgggcg atatcgtgcg gctgtcggcg ggcgatctca tcccggcgga tctgcggctt
    11701 gtccatgccc atgatctgtt cgtcaaccag tcgtcgctga cgggcgaggc gatgccggtg
    11761 gagaaggaag ccatcacgcc cgcccccggc accgacccgc tcgaccggcc ggacctgtgc
    11821 ttcatggggg ccagcgttgt cagcggctat gcggtggggc tggtgctgcg caccgggcgc
    11881 cacaccttct tcggcgagat cgccgagaag atcacccaca ccgccgccac ctccagcttc
    11941 gagaaggggg tcaatcgctt cgtctggctg atgctgcgct tcatgctggt gatggcgccc
    12001 acggtgttcc tcatcaacgg cctcaccaag cacaattggt gggaggcctt gttcttcgcg
    12061 gtggcggtgg cggtggggct ggcgccggaa atgctgccaa tggtcgtgac gatgaacctg
    12121 gcgcgcgggg cgatcgccat ggccggcaag cgcaccatcg tgaagcggct ggatgccatc
    12181 cagaactttg gcgcgatgga tgtgctgtgc accgacaaga ctggcacgct gacgcaggac
    12241 aaggtggtgc tgcgcctggc gcttgatgcc ggcggccacc gcagcgagga ggtgctggcc
    12301 catgcctggc tgaacagcca ccacgaaagc ggcctgcgca acctgatgga cgaggcgatc
    12361 ctggcgcata acgaggtggc gcatcacccg ttgctgacag gccgcatcac caagctggat
    12421 gagctgccct tcgatttcca gcgccggcgc gtctcggtgc tgctgcgcca ggaaggcgag
    12481 gcagagccgc tgctggtgtg caagggcgcg gtggaagaga tgctggcggt gtgcacccat
    12541 gtgctggaag ggggcgtgga aggcccgctc gatgccgcgc gcctggcagc cgttcacatg
    12601 gtgacagacc ggctgaaccg cgatggcctg cgcgtgctgg cgatcgcccg gcgccgcttt
    12661 gccaccgcgc ctgcccggct gacgctggcc gaggaggagg ggctgacgct gatcggcttt
    12721 gtcgccttcc tcgatccgcc caaggaatcg gcggctcagg cgctggccga tctggcagcg
    12781 gcgggcgtgg cggtgaagat cctcaccggc gacaatgatc tggtggccgc caaagtgtgc
    12841 cgcgatgtgg ggctgggcca tgtgcgccag ttgctgggcc atgaagtggc ggcgatggac
    12901 gatgccgcgc tggcgcaggc ggccgaggat gtggtgctgt ttgccaagct gtcgccggcg
    12961 caaaaggcgc gggtgatcgc cgcgctgcag gcgcgcggcc atgtggtggg ctttctgggc
    13021 gatggcatca acgacgggcc ggcgctcaag caggccgatg tcggcgtttc ggtcgatacg
    13081 gcggtggata tcgccaagga ttcggccgcg atcatccttc tggaaaaatc gctgggcgtg
    13141 ctggtcgatg gggtgatcga ggggcggcgg gtgttcgcca acatcatcaa atatatccgc
    13201 atgggggcca gctccaactt cggcaacatg ttcagcgtgc tgggcgcgtc catcctgctg
    13261 cccttcctgc cgatggcgcc gctgcaggtg ctgaccaaca acctgctcta tgatttctcg
    13321 cagacggcga tccccaccga catggtggac gaggaatatc tggccgcccc gcgccgctgg
    13381 gaaatcggca atatcgcccg cttcatgctg ttcatcgggc cgatgagcag catcttcgac
    13441 tatgcaacct atgccgcgat gatcttcctc ttccatgcct ggaacaatgt gccgctgttc
    13501 cagaccggct ggttcgtcga atcgatcctg acgcaaacgc tgatcatcca catcatccgc
    13561 accgcccgcc tgcccttcat cgaaagccgg gccagctggg cgctgatcgg caccacgctg
    13621 gcggtgtgtg cggtgggggc ctggctgccg ttctcgccgc tggcggcaac gctgggcttt
    13681 gtgccgctgc cggcgggata ctggccggtg gtggcgggct tcctgctggc ctatgcggtg
    13741 ctcacccatg gcgtgaagcg ctggttcttc cgccgcttcg gcgacaggta ggccggccaa
    13801 acaaaaagcg cgaggggatc gctcccctcg cgccttgatg cagcttgttg gcgtgatcag
    13861 accagcatgc tgccggtttc gatgaagcgc tggtgccagc tgagggcctc ggccggcagc
    13921 gaggggctct gcagcccgta ggagcccttg agggcgcggg cgtaatagtc ctccagcatc
    13981 gggcgatagt tggggtgggc gcagttggcg atgatcttct cggcccgctg cttgggcgag
    14041 aggccgcgca gatccgccag gccctgctcg gtgacgatca cctgcacatc ctgcgagata
    14101 tgatcgacat ggctggcctg cggcacgatc gcgctgatct tgccgccctt ggcggtggac
    14161 ggcgtcatga agatcgagat gaaggcgttg cgggcaaaat cgcccgagcc gccgatgccg
    14221 ttctggatgc ggctgcccat cacatgggtg gagttgacgt tgccgtagat gtcggcctcg
    14281 atcagcccgt tcatggcgat gcagcccagg cgccgcacca gttccgggtg gttcgagatt
    14341 tcctgcgggc gcaggatcat cttgtcacga taggcgccca tgttggcgtt gaggcgggcg
    14401 gccgcttccg ggctcagcga gaaggccgtg gccgaggcca tgcgcagctt gccgcaatcg
    14461 agcaggtcca gcatgccatc ctggatcacc tcggtgaagg aggtgaggtt ctcgaagggg
    14521 ctgtccacca ggcccgagag cacggcattg gcgatattgc ccacgcccga ctggatcggc
    14581 agcagattgg cagggatgcg gcccagcttc acttcgtgct tgaagaattc catcaggtgg
    14641 ccggcgatgg caaaggcggt gggatcgggc gggctgaagg gggcgttgcg gtcgggcgtg
    14701 tcggtctgca cgatggccac caccttgtcg gggtcgatct gcagataggg gctgccgatg
    14761 cggtcatcgg gcttcaccag cgggatcggc acgcggttgg ggggcaggcg ggtgccgtaa
    14821 tagatgtcgt gcatgccttc gaggtcggcg ctctgccagc tgttcacctc gaggatgatc
    14881 ttgctggcgc ggtcgagcca ggtcttgttg ttgcccaccg aggaggaggg gatcagcgtg
    14941 ccatcggcgc gaatgcccga cacttccacc agcgccacat cgagcgggcc gaggaagccc
    15001 tgccaggcca tcggcgcgac ctgcgaaagg tgcatgtcga aatagttcat ctggcccgag
    15061 ttgatcttct cgcgggcgat ggggtcggaa ttgtagggca ggcggaattc gatgccgttg
    15121 ctcttggcca gcgcgccatc cagctcgggg ccggtggaag cgccggtcca cacgctcacg
    15181 cggaacggat cgccagcggc ttgcgcttcc tcgatgcggc gggccagcgc cagcggtact
    15241 tccttgggat agcccgaacc ggtgaagccg ctcatgccca cggtgtcgcc ggggctgatg
    15301 agggcggcgg cttcctcggc ggtggcgatc ttcgacctaa gtcggtcgtg ggcgatgcgg
    15361 ttggtcatgc taatcctccg ggggtcttgg gccattccgg cctcgcgcgg cctgtgcacc
    15421 cccgggggca caatggcaag cgttgagtgt cgcaaaagct aaaaataaaa accaaaaatt
    15481 tcagataatt acttcggttt tttgagcgat tccgaagtcc gtttcctgcg ccattccagg
    15541 cgtgcgaggc gcgctttgtg ctgcgccgcg gaaatatcct gatatcagac gggaaccctg
    15601 cggggactcg cgcattatga ctgcgcacca accgggccca tgcaggccct gtttcatacg
    15661 tctctgttcg cctaggatcg gccctgctgc tggtcgtgcc gcagcgtgtc tggagtgatt
    15721 gatgttcgat atcgtcaagg tggcgctgca tcgcccgctc accttcatag tcatggccat
    15781 ccttatcgcc atcatgggca cgcttgccgc ccttgatacg ccggtggaca tctttcccga
    15841 aatccgcgtg cctgtcatcg cggtggcctg gcaatatacc gggctcaacc cgaaggacat
    15901 gtccgaccgc atcgtcacgc cttatgagcg cacgctgacc accacggtca acaatatcga
    15961 gcatatcgag agccagtcga tgcagggcat cggcatcgtg aagatcttct tccagccggg
    16021 caccaacatc tcggtggcca atgcgcaggt caccgccgtc tcgcagacgg tgctgaagca
    16081 gatgccgcca ggcatcaccc cgccgctgat cctgaactac aacgcctcca ccgtgccgat
    16141 cctgcagctg gcgctgaacg gcaagggcct gtcggaacag cagctgttcg acctgggcca
    16201 gaaccagatc cgcccccagc tggtgaccat ccccggcctg gccatgccct atccctcggg
    16261 cggcaagcag cgccagatcc agatcgacat cgaccccgag gaactgcagg ccaccggcct
    16321 ttccgcccag gatgtgggca atgccattgc cgcgcagaac cagatcacgc cggccggctt
    16381 cgtcaagatc ggcgaccggc agtatagcgt gcagctgaac aacgccccca attcggtgga
    16441 agcgctgaac gcgctgccgg tgaaggtggt gaacggcgcc accatcaccc tgggccaggt
    16501 ggcccatgtg cgcgacggtt tcccgccgca gcagaatgtg gtgcatgtgg atggccagcg
    16561 cgcggtgctg ctcaccgtgc tgaagaatgg cgccacttcc accctggcga tcatcgacgg
    16621 ggtgaagaac aagctgccgc agctggtgat cggcctgccc gatgcgctga aggtgcggct
    16681 ggtgggcgat cagtcgctgt tcgtgaaggc ggcggtgcag ggggtgctgc gcgagggggc
    16741 gattgccgcc gcgctcacca gcctgatgat cctcctgttc ctgggctcgt ggcgctccac
    16801 ggcgatcatc gcgctgtcga tcccgctggc ggtgctggcg gcggtgtgcc tgctcaatgt
    16861 tttcgggcag acgctgaacg tgatgacact gggcggcctg gccctggcgg tgggcctgct
    16921 ggtggacgat gcgaccgtga ccatcgagag catcgtgtgg catctggaac agggcaaggg
    16981 gctgatcgag gcgatcctcg atggcgccgc gcagatcgtc acgccggcct tcatttcgct
    17041 gctgtgcatc tgcatcgttt tcgtgccgat gttcatgctg ccgggcgtgg cgggctatct
    17101 gttcgtgccg atggcgctgt cggtggtgtt tgccatggtg gcctccttcg tgctcagccg
    17161 cacgctggtg cccacgctgg cgatgtactg gctgaagccg cacacgccgg ccgaacatga
    17221 cgagcatgcc gccggcagcc ctgcctcgcg caatgtcttc gtgcgcttcc agcgcggctt
    17281 cgaggcgcgc ttcctggtgc tgcgcggcca ttatgtgggg ctgctgcgcc tggcgctcac
    17341 ccatcgcaag ccgttcctca tcggctttgg cgcggtggtg gtctgctcct tcgcgctgct
    17401 gccggcactg ggcagcaatt tcttccccaa ggtcgattcc ggccagatca tgatgcatgt
    17461 gcgcgtgccc atgggcgccc gcatcgagga taccgcccgc cgcttcgacc tgatcaaccg
    17521 cgccgtgcgc gagatcatcc cgcagaccga gctggccaat ctgaccgaca atatcggcct
    17581 gccgaacagc gcgctgaaca ccatctacag caatgatggc accatcggcc cgcaggatgg
    17641 cgatatcatg atcaccctgg cagagggcca ccggcccacc gagcaggtgg aaaaccagct
    17701 gcgcaaggaa ttgaccgcgc gctttgccgg cacgtccttt gccttcctgc cggccgatat
    17761 caccagccag atcctgaact tcggcagccc ggccgcgatc gacatccaga tcaccggcaa
    17821 ggacccggcc gccacgcgcg attatgcgca gaagctgtcg gcccgccttg gcggcatcgc
    17881 cggcctggtt gacaagcgca tccagcagcc gctgggtacg ccgcagctga acgtggatgt
    17941 gaaccgcgac ctcatcggcc aatatggcct gaccgagcgc gatgtgacca acagcctgtc
    18001 gaacgtgctg gccggcacct cgcagacggc accggtgttc tatgtgaacc ccaagaattc
    18061 ggtgcaatat gcggtggtgg cgcaaacgcc ggaataccgc gtctccacgc tcgacgatct
    18121 ggccaatgtg ccggtttcgg gcacgggcgg cgtgcccaac cagctgctgg ggggcgtggc
    18181 cagggtcacg cgttcggcca ccagcctggt catctcgcac tataacatcg cgccggtggt
    18241 cgatatctat ggcaacacct atggccgcga cctgggcgcg gtgagcggcg atatccagaa
    18301 agccatcgcc agcctcgcca aggagcagcc caagggcacg caggtgacct tgcgcgggca
    18361 atatgccacg atgaacaccg ctttttccgg ccttggcttt ggcctgctgg gcgcggtggt
    18421 gctgatctac ctgctgatcg tcgtcaactt ccagagctgg gttgatccga tggtgatcgt
    18481 cagcgcgctg ccggcggcgc tggcgggcat cgtgtggatg ctgttcacca ccggcaccac
    18541 gctgtccgtg ccggcgctga caggggcgat catgtgcatg ggcattgcca cggccaactc
    18601 gatcctggtt gtcagctttg cccgcgaaag gctggccgat ctgggcgatg ccacccaggc
    18661 cgcgctggaa gcgggcatgg tgcgcctgcg cccggtgatg atgaccgcgc tggccatgat
    18721 catcggcatg gcgcccatgg cgctgggcat gggcgatggc ggcgagcaga acgcgccgct
    18781 gggccgcgcc gtgatcggcg gcctgctgtg cgccaccatt gccaccctgt tcttcgttcc
    18841 caccgtgttc agcctggcgc atcgccgcta tggcaagacc acttcgcaac cggaagctgt
    18901 tcccgctcat gtctgatcct gtgtcccatt ccgaaaacgc cgagggcgat ctgttcgaaa
    18961 ccgcgccgga tgtcggcaag ctgaagcgcc tgggcgttgc ggcgggtgtc gttgcgctgg
    19021 tggtggccgg cgcgggcgtg gccagccgca tccacgccac cagcgaattg cgcaccgccg
    19081 ccagcgacag cgccctgccc gatgtggcgg tgatcgcccc caccaagcct ggagcgagcg
    19141 ccgggctggt gctgccgggc acgatccagg cgtggaacag cgcggcgatc aacgcgcgca
    19201 ccaacggcta tgtgaagcgc tggctggccg atatcggcga tcaggtgcgc gccggccagc
    19261 cgctggcggt gctggatgcg cccgaggtgg accagcagct ggcccaggcc gcggctgact
    19321 atcagaccgc gctggccaac cagcgcctgg ccgaaaccac ctcgcgccgc tggcagctga
    19381 tgctctcgca ggatgccgtc tcgcggcagg aagcggatga gaaggcgggc gacctggcgg
    19441 ccaaatcggc gctggccaat tcggcgttgg ccaatgtgaa gcgcctgcgc gcgatgcagg
    19501 gtttcgagcg cctgtccgcg cccttcgatg gcatggtgac gagccgctcg gcccagatcg
    19561 gcgcgctggt atccaccacc aacgccagcg ccccgccgct gttcaccgtc tcggatgtgc
    19621 atcgcatgcg catctatgtg cgggtgccgc aaagctattc ggcgatgctg catgccggcc
    19681 tcaaggccac gctgacgctg cccgaattcc ccggccggca gtttgccgcc gagctgaccc
    19741 gcagcgcggg cgcggtggat ccgcaatcgg gcgcggtgct ggtcgagctc cagtccgaca
    19801 accctgaccg ggcgctgaag ccgggcgcct tctcgcaggt gcgcttcgac actggcgctg
    19861 tgcagggcaa cacgctggaa ctgcccagca gcgccatcat ctatggcgcc aatggcccca
    19921 gcattgccgt ggtcgatgcg cagggcgtgg tcagcgtgcg gcaggtggcg atcgcgcgcg
    19981 atgaaggcaa tacggtgctg gtgggcggcg cgcttgccac caccgacaag gtgatcgaca
    20041 gcccgcccga tgccgtgaag agcggcgaca aggtgcgcgt gacccagacg gaaaccgcag
    20101 ggggcgccgc gcatggcaaa taagctgcgc aaagggcgcg cgccgctggg cgtggccctg
    20161 ctgaccagcg ccctgctggg cggctgcgtg ggagcgccgg cctatcaccc ggtgaaggtg
    20221 gcaacgccgg cccaattcag cgaaacgccc gatggctggg ccgccgcgca gccggccgat
    20281 ggcgccgcgc gcggcccctg gtggcaggtt ttcgccgatc cccagctgga ccggctggag
    20341 cagcaggccg aaaaggccag ccccacgctg gaagcggccc ttgcccgcta tgacgaggcg
    20401 cgcgccaccg cgcgcgtggc cgatgccgcg cgcctgccgg aagtggatgc ggtgggctct
    20461 gccgcgcgcg acaaggtgtc gcagaaccgc ccgctgacga ccgggcgcga agcgacctac
    20521 aacgactatc gcgccggcgg cctgctgacc tacgagctgg atctgtgggg ccgggtgcgg
    20581 gccgccgcgc gcgccggcca tgccgaggcc gatgccagcg gccatgacct ggccgcggcc
    20641 cgcctcagcc tgcaggcggc ggtggccgat gcctatgtgc ggctgcgcgg gctggatgcc
    20701 caggccagcc tgctgcgccg caacgatgag gcctatagcc gcgcctatca gctgaccgcc
    20761 acgcgccacc agggcggcat tgcctcgatc atcgatgtga accgcgccag catggtgctg
    20821 gccaatgccc gcgcccagct cgccgccgtg gaagaccagc gcgccgccac cgagcacgag
    20881 atcgccgctc tggtgggggc ggtggccagc aatttccacc tggccgccgt gcccgatgcg
    20941 ctgcgcgtgc cgctggcacc ggccaccacg ccctctgtca tgctgcagcg ccggcccgat
    21001 gtggccgccg ccgaaaggcg catggccgcg gccaacgccc gcgtgggggc ggcccgtgcg
    21061 gcgctgttcc ccaccatcac cctggggctg accggcggct atgaaaccac cgggcccagc
    21121 atgttctcgg cgcccaatgc cttctggggg ctggggccgg ccagcgtgtc gctgccggtg
    21181 ttcgatggcg gcaagcggcg ggcgcaattg cgctctgccc gggcgcagta tgacgaggtg
    21241 gcggccacgt accgcagcac cgtgctgacc gccttccgcg aggtggagga ccagctggcc
    21301 gccagccgcc tgctggccca tgaagccacc cagcagagcc tggccgccga tgccgcgcag
    21361 cataccagcg acctgtcgtt cacccgctat cgcgaagggg cgaccgatta cttcgaggtg
    21421 gtgaccgcgc agaccgatgc gctgaacgcc cagcgcacct tgctggccgt gcagacccag
    21481 cggctgcagg cagcgattgc gctggtaaag gcactgggcg gcgatccggc gccggctttg
    21541 ggcggggagc ctgcgcccgg caagcagggc tgataggggc ggcgaggggg cgttaccccc
    21601 tcgccgtcgc ccttatagcg cccacatccg cacgaggttg tgataggcct tggtgaggcg
    21661 cagcacttcg ctatcatccc cgccgcgatc gacagagagg cgctggatcg cctgatcgag
    21721 atcgaacagc gcctcgcggc cggcatgatc gcgcaccagc gattgcagcc agaagaagca
    21781 ggaaatccgc tcgccgctgg tcacctgcgt tacccggtgg aggctggagg aggggtagag
    21841 caccatgtcg ccggcgggca gcttgtgggc cactttgcca aagccgctct cgatggtcag
    21901 ctcgccgccc tcatagtctt ccgagaggaa cagcgtggcc gacaggtcta tgcgcagctg
    21961 gcggccggtg gcccggtcca gccgcacggc attgtccaca tggtcgccaa agaattcgcc
    22021 cgggccatag cggttgaaca gcggcggaaa cagctgatag ggcagcgccg ccgagaggaa
    22081 cagcgggttg cgcgccagcg cccgctgcac aatggcgcgg ccccgcgcgg cggcctcgcc
    22141 ttcctcgggc agctggcggt tgcgcttggc cagcgcggcc ccttcgccgc tggtgacatt
    22201 gccatccacc caggcggcag cggccagttc acggcccagc gccagcgcct cttcgcgggg
    22261 caacacttgc gggatgacga tcagcatgga gaactccctt ctggcggggc tattaatgcg
    22321 atccatttgc aatagcaagg ctgttgcgaa cgcctttcaa caagccccta attggccgga
    22381 tgataaggcg cgcgccaggg ctgcaagacc gctcgcgggc catagccaaa ggcgcagccg
    22441 gccacatcat gcgggccttt tgcccccagg cgcgcggcca gcacttgccc cagccgggcc
    22501 gaaatggccg cagcgccagc cgcgttcaca tgctgcgggt cgttccacag ctctggccgg
    22561 cgcaggatat cgttcacccc caccatcgcc cgcccgccat accagcgcct gtccatcggc
    22621 tggtccggcc ggccgaaagc gggcagatag agcagcagca ccggcgtatg cgtgctgtct
    22681 gccagatcga gcatgccggt gagatagcgg cgggtgaggg catattccca ccggtcgaac
    22741 gccgggggca gtatgtgcag gcgcttggcg gccatatcgg cagcggcggt ggcgcgcagg
    22801 tcgcttgcgg ggaagacgcg atcccgcagc ggcgtcatct cctgcccgtg gacattgacg
    22861 atgcgcgtat tgtccaccgt cgagccatcg taatccgccg cagaccaggc tgctttcagg
    22921 ccgaattgcg ccggcgccag gctttgcgcc cacagcacca gctgccgcca gggcaggcgg
    22981 atgacatcgg ccatgtaatt gccgttgacc actttgggcg cgcccagcac atcggccgtt
    23041 tccgccacgc gatagaacag cggatgggcg gtgcgcgtct cgttctccag cacttccagc
    23101 acgagcaggc gcggatggcg cgcgcgcagc acttcgcgcg ccacggccca ttgcatgttg
    23161 cgcccatagg ccgggatcgc cagattggtg acggtgaggc agcggccatc gggcgcccgc
    23221 accccggcct gcgccagccc gcgcgccacc gccaggccat ccaccccgtt cagcgtgtgc
    23281 gaagtgccga taaaggcgat gtccatcggc gtggggtcat cgtggatgcg ctcatacacc
    23341 cagccgaggc gggcaaacgc ctccgtctgc tccgactgcc agcggatata ggcgcggtgg
    23401 ggcagggcca tcatcgccag cagggtggcc agcatggtgg caaggccaat cgcgcagagg
    23461 cgagggaagg aaggcgccat ggtgagaccg ctccgctcag aaattgaaat agaggaacac
    23521 gcccggatcg ggcaggcggg caaaggcaaa ggccgccacc acccccagca ccgctgccac
    23581 cgcccagggc cgcgggcgcc agcgcagcgg ctc
//
LOCUS       DASRYE010000290        31351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_32038,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000290 DASRYE010000000
VERSION     DASRYE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 31351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31351
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32038"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFF98_14780"
     CDS             <1..880
                     /locus_tag="VFF98_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64948.1"
                     /translation="GLRGSTAASAVSYRHTDQPVWRGSFDVCDLRAQVGRLFGDGSTQ
                     QGHAVITAIVETAIAWADKVKEEGKAHQLTTNVIKTLERTLRSFMDFRTGACEASYDL
                     ISKAAYFGRITVIRHMKILRDLKWFDWVRRTEPTGNGPKEGPRVKQAANAYFFEISRL
                     PVAAQIHLRQILKRRGIRLDEHPERQGSGPVPNRIQRLASRVSQALSGARGSRRSPAE
                     KAALIDEAAFVRAEMAFFGDIPTDRWAALRHPQDPAAQAAYNARLGIDLCATASTGSS
                     PESPLQNGKDKEFGSR"
     gene            complement(1061..1873)
                     /locus_tag="VFF98_14785"
     CDS             complement(1061..1873)
                     /locus_tag="VFF98_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HZU64949.1"
                     /translation="MKSTLDSVRPVSPAAGYQGGKRNLARRICSLIDAADHDGYAEPF
                     VGMGGIFLRRSTRPGVEVINDISGDVATFFRVLQEHYPYFIDMLRFRVASRAEFDRLL
                     ALPSERLTDLQRAARFLYLQRLAFGGKVTGRNFGVDKRAGARFNITKIEPLLADIHDR
                     LAGVVIEQLPFDRFIERYDRPGMLFYLDPPYWGCETDYGAAVFSRADFARLADLLRGA
                     RGRFLLSLNDLPEVRECFGAFHIIPVETTWTIAAAHGGAQRVGEVIITNLAA"
     gene            2071..2319
                     /locus_tag="VFF98_14790"
     CDS             2071..2319
                     /locus_tag="VFF98_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZU64950.1"
                     /translation="MKPITYTRAALKTLRRIPANTSALITGKIEQFASDPASLANNAK
                     ALTGRQGIRLRVGDWRVIMDDGVVLAVLEIGPRGGIYD"
     gene            2334..2675
                     /locus_tag="VFF98_14795"
     CDS             2334..2675
                     /locus_tag="VFF98_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU64951.1"
                     /translation="MGEMISIPVEEYRLLRSAAQELEELRTYDGAKAALAAGEEELIP
                     AEYVKRMLAGESPLRVWRDYRGLTQLQLSEAAGVNRVQIAEIEAARKVGSIETIKKLA
                     VALGITIDDLV"
     gene            complement(2676..3878)
                     /locus_tag="VFF98_14800"
     CDS             complement(2676..3878)
                     /locus_tag="VFF98_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HZU64952.1"
                     /translation="MLTLAAVKAAAPQARAYKLFDSGGLFLFVAPTGLRAWRWKYRRG
                     GRERLLSIGRYPEISLPEARARRDTARARVAAGADPASVAFQVNTLEQLARSWHAHQQ
                     EQWSPAHAADVLASLERDIFPALGGRPAADIAAPELLDALRAVEGRGRIETARRLRQR
                     LSMIYAYGVAEGLIDSDPAARLGAAMRKGRPPRPQPSLGTVEACRALLAACNALAGRP
                     IVALASRFLALTAVRLEAVRGMRWGEVEDLGGPAPLWRIPAARMKLARAKKGEQRFDH
                     LVPLSAPAVAVLRQVAHQQGQDGRASPLAAETLVFPGASPGRPLGENAIGALYARAGF
                     AGRHVPHGWRASFSTILNEVLPDERAAIDRALAHSPKDKVEAAYNRAQQLARRRALFE
                     HWGALLAG"
     gene            complement(4664..4945)
                     /locus_tag="VFF98_14805"
     CDS             complement(4664..4945)
                     /locus_tag="VFF98_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64953.1"
                     /translation="MKARAEKPMEKSCFYLLDDKQNADLESYRYALRALSQLACQSDG
                     EEKDAIINRFNLRCLFDQLEKRLADIICDGNLESEWLERPSTIHVKTFQ"
     gene            complement(5462..6391)
                     /locus_tag="VFF98_14810"
     CDS             complement(5462..6391)
                     /locus_tag="VFF98_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64954.1"
                     /translation="MATYGVNIIKSLISSNDASKPLLTRDPVVNGANGGLLFGFDLAF
                     PYSYPGGYAGNELQRPAAWPPTNGQTVYDFSGNGNNGSAVVASTAPAIAGGGFDFSAV
                     PVAANGAYVAAPASVMAALYASQGTQNPQYIICGYFKLPTLANWITDSGVVATIMGCT
                     SGYNSGAILSEVEITQQNNGLKGICQTGSGSSASVYVGIPRDFGSLAQIALYRTNGGP
                     TMMSIQTASGIATANLGTTATTQNYSASQLLWGMGPFWNSSNASEANARAFRLYRGWV
                     ENLAVSGRNPAAVLAADWQAVLNRISAGIMAFS"
     gene            complement(6407..7909)
                     /locus_tag="VFF98_14815"
     CDS             complement(6407..7909)
                     /locus_tag="VFF98_14815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZU64955.1"
                     /translation="MSSDITAQGLAVSQATAANNNTGKASPSYFETLNLNAADGIVVD
                     PSGRVINAGGVTDAQKAAIMAKVSHATTAWWETTDASTRNGAIVDADGRLIGTDDMGG
                     VVASAAGSRSDLPSRLNVGLTPYGSPNIPIKMAYNLRHTRRNIRARLCNDSAITQIRW
                     LGLGDSYTDGFSYWSSSLSTVDFPAYNGGTANSIGYSSPGWVGFGSSTGASGGIKGTP
                     APWLYSCVRSGTWTDLFGSDAYSPDICSAYSSTAGDTYTIASASSSNPVQTIAKLFWH
                     GTGSVSYAWNGGSATTINVSSAAALAITALASMPATATWSLVITVVSGTPYLCGVQFD
                     NATVNGMQFFKCAATGQKISGRAAGDATSFATALASFAPNIVSIGTPINDRNAGRTAA
                     QYYTDVQTIISQCRAAIPDIDILLFVPAEISAGYPYWAPMSYYAAKMEQLAAEQSCAM
                     IDFQKLFGADPTVYGPSGVFPLLEATGVHPSITFGARALQDGFIRALVQN"
     gene            complement(7945..8526)
                     /locus_tag="VFF98_14820"
     CDS             complement(7945..8526)
                     /locus_tag="VFF98_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64956.1"
                     /translation="MPFLRFLAALLALAIATPAAAQVTLPQIKNAGGASVPSTGAVII
                     DSSGAEKGTSANPLYITCWVGGCYSTIAGPSGNQAAVDTSSRLSVSVNNTPYVQPLYA
                     APASYTTGTWFDARAYGSVTFTAVAAATAAYSPKWSPDGGTTVAAMTCIDLNFTTQTS
                     FGATAATAITCKGGGYLELTGGTGGTFMLNAGQ"
     gene            complement(8663..8926)
                     /locus_tag="VFF98_14825"
     CDS             complement(8663..8926)
                     /locus_tag="VFF98_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64957.1"
                     /translation="MRSFLMLPALAPMLMLASCANLLSPPPQAAVPALTPPLMPTPKA
                     TGAIDPLCAVLRPIALSHADTDGTKVQAIALNSVIDKACGPAA"
     gene            complement(9073..9444)
                     /locus_tag="VFF98_14830"
     CDS             complement(9073..9444)
                     /locus_tag="VFF98_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64958.1"
                     /translation="MAIKIFGLTILTSTELASVKSFALSEATKAVAALKETPVGTAIA
                     NAISDIDNANLTGIQKFEKVLSDTLPQLQQLLTAQGLKAELADLEDLGRSLVQTIYND
                     FKSTTAGTWATSILKLLGISA"
     gene            complement(9506..9679)
                     /locus_tag="VFF98_14835"
     CDS             complement(9506..9679)
                     /locus_tag="VFF98_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64959.1"
                     /translation="MDKDFSDLVVLVAGIVITGLATMTAEHMGVAGAHEVGVMIIGGL
                     LALARNRAPASAA"
     gene            complement(9707..10261)
                     /locus_tag="VFF98_14840"
     CDS             complement(9707..10261)
                     /locus_tag="VFF98_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64960.1"
                     /translation="MQLTPRILAYVASEEAIGLSAYLDPKGIWTWSLGIAESGGNAVR
                     QYRDNPQPLEACLQAAIALLQTGFVPVLAKAFAGQALAEHQIAGALSFAWRNGPGNLL
                     TAQWVKDVLAGRPTSARASFMQWTDHGRQLARATRERDLFFDARWPADLRVRIFAAKG
                     PQYQPVGGRLTNVLPQLQQIMGGR"
     gene            complement(10304..10531)
                     /locus_tag="VFF98_14845"
     CDS             complement(10304..10531)
                     /locus_tag="VFF98_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64961.1"
                     /translation="MPETELYLELGGLKQSMTNVEAAVEKLITKVDALAGEVAELKLR
                     EARRSTIEKVAMWISGVFGAGSAIVLEHLWK"
     gene            complement(10602..11138)
                     /locus_tag="VFF98_14850"
     CDS             complement(10602..11138)
                     /locus_tag="VFF98_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64962.1"
                     /translation="MTAYIDQYFKDAAGAVHFLSAQDQANAAAPGWAGPTLPDPAWTA
                     IAADEAHTIANPPPSAAQLLAYAQARRQVVADGGITVNVGTTAAPVACAANTSASGVS
                     DLAVMLLQAEASGSTTFYQSGGDVTVTTAQIQTIQQAVASFIGATWATLNTVSAAISA
                     GTVATTAEIDAASWPATS"
     gene            complement(11142..12149)
                     /locus_tag="VFF98_14855"
     CDS             complement(11142..12149)
                     /locus_tag="VFF98_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64963.1"
                     /translation="MFRIDASTAAAALPAPAAAGTPGYFTGGNAGTGVPPTTITADWL
                     NLIQEELMAIVNFAGLAPSKTATNQVLQALQQNFSAPFQTVFAGVASNALTLGWTPAG
                     PLWFRSATLTSGAPVAYSPSAPASLALTVPSGATLGTIAGQQATLALLVLYNGGAPVL
                     AVVNIAGGVNLDETTLISSTAISSAATSAGTVYSAAAVAASPFRLVGYVQISEAVAGT
                     WATAPALVQGRGGEAMAAWQSLGFGQTWQNVTASRVSGTTYYNTTQRPIFVQVVFPDT
                     SGGSPNITITVGGVTLVNNLNYDYSSGTATAIPSFIVQPGQSYSVTISNGSIGQWNEL
                     R"
     gene            complement(12174..12704)
                     /locus_tag="VFF98_14860"
     CDS             complement(12174..12704)
                     /locus_tag="VFF98_14860"
                     /inference="COORDINATES: protein motif:HMM:NF021583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HZU64964.1"
                     /translation="MARSTSDYAGALKALLPQGRVWRVEQGSGQEAVLNALGAGWARL
                     DAAAEELLDGSLPGQFADMLPEWEETLGLSGSISGLTTAQRSAQVLSRFIANGGQSIA
                     FLTSFAATLGFAITITTYARCRAGIATAGSPVYAENWASALGIHITANTSGLASSALI
                     AALSPIISAHVAIFLV"
     gene            complement(12708..13853)
                     /locus_tag="VFF98_14865"
     CDS             complement(12708..13853)
                     /locus_tag="VFF98_14865"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HZU64965.1"
                     /translation="MPYSRPSLSSLRSQVQGDIDGNLPGLDTLLRYANLRALGAASAG
                     VANGCYGYLDYIAKQSNPFTATGEALAAWGALKNVYLKEATAASGAITFAATAGTTTP
                     IPAGTTITRADGATFTTTAAASPSGTSVIVQATATTAGAAGNTAAGTAMYLSVGLSGI
                     TPNGSVSTAFTGGADVETQDDFKSRVLVAYQEEPSGGSVTDYENWALAVSGVTRAWCV
                     ARAYGPGTVVVLFMMDEVRAAENGFPQGTNGVATSETRDTAATGDQLLVANALFSEEP
                     AEALVYAFTPTPNTITLTISGLASAGSTVQAAVQAAFASAVTSKGAPGGTLDLSDIEA
                     AIAAVSGAAGFVITAVAASAGTVTPGPTGNITSNALALPAAGAITFV"
     gene            complement(13855..14370)
                     /locus_tag="VFF98_14870"
     CDS             complement(13855..14370)
                     /locus_tag="VFF98_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HZU64966.1"
                     /translation="MTDIQTGWSNAAGSGDWIFVPAAAALQAVGLGASLVGATGLTVQ
                     AELDASTSHQWVADLVTAVLISLFSDAAADDDDVIPDGTANRRGWWAGDIGSKLWLLS
                     RVKATAGVATQAEGYARDALAWLVTDGIASTVQVSSQWLSTSALALTITITRASGTAV
                     TLAFANLWDFL"
     gene            complement(14367..14939)
                     /locus_tag="VFF98_14875"
     CDS             complement(14367..14939)
                     /locus_tag="VFF98_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HZU64967.1"
                     /translation="MTVSRPIANMVGAGNVTGVDDSGETQRLQITEGAVGSGFMARVL
                     DGVRRIFSFGFTSVPPLGSEVVMLRRGGQRVCSIIIGTNHRASRPTGLSPGDTAMYDV
                     RGVILKFTAAGPVLDCAGLPLIIQNASKMTIDVPDVELTGNLTVAGDITGLNGSAALA
                     LSTIRSTFNAHGHTGVSTGSGTSGSANHSL"
     gene            complement(14936..16078)
                     /locus_tag="VFF98_14880"
     CDS             complement(14936..16078)
                     /locus_tag="VFF98_14880"
                     /inference="COORDINATES: protein motif:HMM:NF044847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64968.1"
                     /translation="MSTDLTGPDIVVVANKPVAATDDLTLIVDGTEIAGWEDIEVTLR
                     TEGFPPTFQVTASNGPTDQLPVEAGDPCVVKLGADTVITGYVDRVRDYGDANTHRIEI
                     TGRGKTSDLVDCGAEWPSHQMIGGNALIIAQRLAQYYGINVVLANGASPGPDVPSWPL
                     NYGETAADIIQRVARNAALLAYEDSLGNLVLANAGSTQAASGISWGVNVQAWSVERSM
                     DQRYSEYICVGIAADTEGDVQGSDLFSAVFDPNVPRHRLMYLIEEYGAANPEDFTEKR
                     AKWEMARRAGRSNVVLATVDSWRDKAGKLWAPNTLMPVWLPAATTGEKLILSEVTFKR
                     NNETGTTADIVAMPSLAFTPEPIVLQPVSAADVKPVNADAGQGGNQ"
     gene            complement(16087..17646)
                     /locus_tag="VFF98_14885"
     CDS             complement(16087..17646)
                     /locus_tag="VFF98_14885"
                     /inference="COORDINATES: protein motif:HMM:NF018818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HZU64969.1"
                     /translation="MSLTSSASTLALQAVNKLTSGKAAGGAAKSSAVQGQAGASSSAT
                     KRTYTPVAPASWRGISFAVTSTETNGGRRVALHQYPGKEVGWAEDMGRQAERYVLRGF
                     VVDGDVTFGGAAIALQRAQLVSACEKKGTGTLVHPTMGSLTVMLERWSIGEGLEASNY
                     SEVSLEFIASGQQAYPTSTAAAATSTTSTTTASGYTKTTQTTKAASKLKTALIKIAAS
                     TVATLVSGGSISTSTILAEATGVLSLAGVRADIVGAIAGWSTKIIAFAQDATALFRIS
                     AVLPAASGTSYGRYSAGANAGLTGSNPTGYTPDTTIDDLITDASAEQAAVSTAASTLA
                     STAAAADLTDISDVGTAATALIEALLACCADPADAIRILLGVLSASLTGLSTSATQVI
                     TTMIWRAAAAALTDVVAQYQPSSLDDAAARLAQIAPVLDALAVAAADAGEDDAWAALD
                     ACRNQIAASLRAASATLGQLTTFAFGQPLPALLLAQQIYADSSRADQLIAQADPVHPL
                     FMPATFQALAS"
     gene            complement(17717..19474)
                     /locus_tag="VFF98_14890"
     CDS             complement(17717..19474)
                     /locus_tag="VFF98_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZU64970.1"
                     /translation="MSTDISGLNLPISASDDTQAGIKAAEKRLSRFASNAEKLGKRLS
                     QAQRQAAQQAANDDDKQTRTRERNLRRFAQRTHTSFKMVEQGLARAFGSKTTISGGIG
                     DMARRTGDGFGALKAAFVETGEGAGVMEAALGTVAGTIAAVGAAAVIGTIAVQKFGAS
                     WAEQTAKVSRFATTMGVGKQALQEFMAAAEQMGMDKGAAQGAMGSLSETLNDAKYGRN
                     GQARDMLRRTGIQFQMNKDGSVNTDAMVPAIADAIKRQNSSGRRTLARAWGIPMEAIP
                     VFAQGGKALSDQMTDAGRYANVQSDQNLSDAERLQQKNTRLSQIKDRVEAQGGHFAAT
                     STEGLLDGELSAARTFADAVSGNFKPAVNALRDAAKGFGDAVDHFLLATNPLAWTALH
                     TIHRLMGGKPDGQGGAQAAPVPTGNLPDSIERNGEQSRQGQVSPKGAVGVMQLLPSTA
                     QMVARRTGIPWDPWRFRTDEAYNRDLGQKYVGMLAKKYRGDQTLAAAAYNAGEGNVDN
                     WIRTNGDPRKGHISDAQWAERIPFRETRDYVHRVIGGVAMQPTPTNVHLLVEARNLPP
                     GTRFTARSDKGAVSQSIPN"
     gene            complement(19592..20005)
                     /locus_tag="VFF98_14895"
     CDS             complement(19592..20005)
                     /locus_tag="VFF98_14895"
                     /inference="COORDINATES: protein motif:HMM:NF021614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HZU64971.1"
                     /translation="MADATEQAASGEDILAAIADQLAAFGRRVAECDLSENEANALAA
                     LLPLVDALKKRLDPPEALEIPLAKPVELGSESYPLLRLREPTAGEMIELDKFTGWEAD
                     VKAIALISGVPEAAVRKLGVRPVRRASEYLGRFLR"
     gene            complement(20007..20366)
                     /locus_tag="VFF98_14900"
     CDS             complement(20007..20366)
                     /locus_tag="VFF98_14900"
                     /inference="COORDINATES: protein motif:HMM:NF022086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HZU64972.1"
                     /translation="MSAIAGTLSITVDGVTYAVAGQGTYLVSSTTRETLTGQDAVHGY
                     SEKPAAGHIAWQGRDSGAVSMATLNSANGVTVVASLINGKVIIAQNAWRDGEPAEVNT
                     EDGTFSIRFASASVTEN"
     gene            complement(20377..21891)
                     /locus_tag="VFF98_14905"
     CDS             complement(20377..21891)
                     /locus_tag="VFF98_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HZU64973.1"
                     /translation="MTVPFTLTPSNLRVPLFSAELDPSYANTATTVQRTLLIGQMTSA
                     GTFTANTPVRLASVSEAIAGAGFGSILAQMAGYYGNADSAGEVHALPLSDAAGAAAAT
                     GTITVTGPATAAGTIALYVAGVLVSVPVNSGDTASVIAANIAADINASSGQSISTMIS
                     CGLPVTASAAAGVVTLTARNAGLAGNDIDVRVNYRGVAGGEALPAGVGIAIVAMAGGA
                     SNPAITNALAALVDKTYDFIVLSLTDSTALAALKTFLSDATGRWNPLQQLYGHGWIAL
                     RGTAGTVAAATAARNDQHLTAIGFYDGPNPSWAWASAFAGAAAQSLRNDPAVPLQFLT
                     VPGLLAPLQASRFPLTVRNTTLLYSGCATWTVDASGNVMIENIITTYTTNSQGAADNS
                     YLEVETLYTLMYVLRYMKSWVQTKYARVKLAADGTRLPANSNIVTPSTIRADIIAAYQ
                     TLETDGFVQQSSVFAANLVVEKDATNPNRVNVLWPAVLIDQLRQFAMLAQFRLS"
     gene            complement(21898..22149)
                     /locus_tag="VFF98_14910"
     CDS             complement(21898..22149)
                     /locus_tag="VFF98_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64974.1"
                     /translation="MRVVSVPGRLVRHPVTRRVIDERGVLVDPHDVTFARLVADGDLA
                     VAEDDEVPEVADAAPGASPAISADASAGTSAAGAAGAGE"
     gene            complement(22160..22735)
                     /locus_tag="VFF98_14915"
     CDS             complement(22160..22735)
                     /locus_tag="VFF98_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64975.1"
                     /translation="MPWAAPQDPVTSDSLLSLFLACLTADGATAAGSRVYKPGDWPAQ
                     SGQLPVIKLRILRENRQSAGRGTVMYTTTATIRVMGEVESYAEPDNAGAAAAEGACWA
                     LKRQIEVAIVNSYALFMRIQQIASIDSQLAYNSEGRTHVAAIIMDFAVEFFEDSDSFA
                     AIAADDAADVLLSATNYAPDVPVSADISLNP"
     gene            complement(22735..23046)
                     /locus_tag="VFF98_14920"
     CDS             complement(22735..23046)
                     /locus_tag="VFF98_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64976.1"
                     /translation="MVLSPVMGIFGSGIVFVPAGLTPIPLPDAVLDEEASVTVPGDDG
                     MAITVQKPVVGIRVAALGGYVPQQGDRVIDQASGRTYIVSNPLSDGHGHVLCHLMWTG
                     A"
     gene            complement(23092..24117)
                     /locus_tag="VFF98_14925"
     CDS             complement(23092..24117)
                     /locus_tag="VFF98_14925"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HZU64977.1"
                     /translation="MVSLDIFHQDPFSTFQMTAAFERIPYLPSQLGDLGIFEPYPIRT
                     TALGIENRDGVLNLIQSSQRGAPPSQDRTTEKRMMRYFEVPRLEQGDTIYSHEVQNIR
                     AFGTETEMMQVEAEVARRVAGPTGLIPRLQYTWENMRLGAIQGVLLDADGSQLYNWFD
                     EFGVAQPAEVAFNLTANVEFTLRGICNKVVRGMWRAAKGAFTTETKIFALCGDAFWDA
                     LTQHVDVVKTYYNWQAAQELRQGYAFAAMEFGGIHWFNYRGSDDNSTIKVPDTKVKFF
                     PVDAPGIFRQALAPGESFEWVNTPGKDWYLIPIFDRDRKFWWRVEAYSYPLFICTRPE
                     VLWSGAA"
     gene            complement(24217..24876)
                     /locus_tag="VFF98_14930"
     CDS             complement(24217..24876)
                     /locus_tag="VFF98_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HZU64978.1"
                     /translation="MGNPLVTPISELWHGWGFLVWEPSDGIYTREQISLAAGYGIITA
                     GTVLGALLTGTATEVALGTNTGNGTFGAITVGTGAAQGNYVVEFDDATHFIVSSPTGV
                     EIGHGTVGVAFSAGGIGFTITAGATAFAPADGFVITVAGTLTYAPYDPTQTNGLQNAV
                     AILGSGRKDTTNAAQKAAGVVRGPTVVNASELVWGANVTTAAQQQAGLAGLLANGIRA
                     R"
     gene            complement(24932..25702)
                     /locus_tag="VFF98_14935"
     CDS             complement(24932..25702)
                     /locus_tag="VFF98_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64979.1"
                     /translation="MTSRLMAGTSRFAHLAGFGGRRSRRAADERDQGDDRQGRRAEED
                     DEDEDEDEDGQREGDGDARRSRRSRRADGDGPGEDEGDPNADPEDNQRGARRARRADG
                     DGGDDGGDEGDDDANARGSRRSRRAEADEDDEDEEDEREMNGRSAAASARRRERVRCC
                     AVLEAVASAMGNPPELEIALTQMESPIGRHRAVRSVSRAARSMEGRQRDNGQREARSD
                     RSARNPGVGGTAPQEPRGAAVQRGWGAAIDGVIGKGRR"
     gene            complement(25771..26604)
                     /locus_tag="VFF98_14940"
     CDS             complement(25771..26604)
                     /locus_tag="VFF98_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZU64980.1"
                     /translation="MLPFALATRLFNTPVALAPQAAEMAVRLLKLASASDMEMLGRDT
                     SGAVDGALQVVEGVAIIPVKGTLVQQVGDLWWLAGLFGISGYDRIRALLLDALANPKV
                     RAIVLDVDSPGGEVAGCFDLADTIYRARKAKPIWAILGENAFSAAYALASAAEFITVP
                     RTGGTGSIGVIMMHVSFAEALKMAGVEVTLITKGDLKAEGSEVIKLSNEAFKRLKADV
                     ETVGDLFDATVARNRALPRKKVFDTQAGTFLGADGVDYGLADAVMAPDEAFRTLLTEL
                     G"
     gene            complement(26604..26942)
                     /locus_tag="VFF98_14945"
     CDS             complement(26604..26942)
                     /locus_tag="VFF98_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64981.1"
                     /translation="MTMFFDATRLGGRRSVRIAAAAVAWLDETDGCAAIHLIGGEVIR
                     VCEDSAEIEARMQPPPCAADAMADQSPASAAAAAPSSGPSSGPSSGHARAADFRAHRH
                     GRPSATRVPA"
     gene            complement(26939..28603)
                     /locus_tag="VFF98_14950"
     CDS             complement(26939..28603)
                     /locus_tag="VFF98_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZU64982.1"
                     /translation="MSGTPRVYHPNGNLIPAGEIARIREQAAGPRGRMRALNGPAPQW
                     FPYDAQDFTSAETADWVPWINSPDREINLYRDRVTARARDIYRNDAWAKGAIGRLLDS
                     TIGGHLRLVVKPDYRALGFDAEWAEVYRQAVEAHWRSWSQDLRRFNDLARRHTMTQMF
                     RLALGHKLVDGESIILSHWRDDRRLEQGAAFATCFQGVDPDRLSNPFQGPDTRYMRGG
                     VEVDDDDVAIAYHIRKAHQNDWYNAVESMEWERVAREDPDGWQRVFHDYDPDRFQQHR
                     GMSIFAPVLGRLRMLAKYYNVELSAATVASAFGLYVTSPFDLDMVRQALDDDDQDEMA
                     WGWYQNLRTDFHRDRDISVSGAKIATLAPGEDIKTVAPVRPNSGFSSFTHEMLRSMGQ
                     VLGLSAEQTHNDYSESSWSSARAGIVESEKMFRRRLADFFVNTSTPVYGTWLEELHDR
                     GLVPLPRNAPAFAEARTAYARCQWLGAPRGWVDPVAERQGAVLGLDAGFATLEQVCAE
                     QGADWEENLDQRAREYHRMRELGLPQPEWMGADRAANAVAAAPEAE"
     gene            complement(28600..28863)
                     /gene="gpW"
                     /locus_tag="VFF98_14955"
     CDS             complement(28600..28863)
                     /gene="gpW"
                     /locus_tag="VFF98_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HZU64983.1"
                     /translation="MSLSHPTVLAGIPPATLQQWLASAQLALFQLQSGQQVASASYAQ
                     GDGAKSVSYRQADMASLNMLIRQIQQELGIIRRARRPARVWFR"
     gene            complement(28912..30987)
                     /locus_tag="VFF98_14960"
     CDS             complement(28912..30987)
                     /locus_tag="VFF98_14960"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HZU64984.1"
                     /translation="MPEWADAKRVKPRGTGSSAGAWRTSDVEIARGPLLAVTEPGVHI
                     LSCMVATQLLKTSLIENTFGFHADLDPCPMMIVQPKDDAAEQFSKERVAPFISATPAL
                     RKLVGTGKTRSAEETITYKAFPGGFLALVGGGSPDNLARRPLRIILYDEIDKYPITRE
                     GYAIDIGDERLASFANWLSIRVCSPTVKGESNIEASWLESDQRRASIACPHCQHRQFL
                     DFFEHVHWDKADGGRTHLPHTARINCEGCNKEWSDAERLRALATIRWHQTRPFECCGE
                     RQLPLDAYASCWRGGHAAPVGQIWDWWASNRWGVYRARCAHCGAWAVSNEHAGFQASK
                     LYSPWSKDNPGAIAKKFLAAQGNEDKLQVWWNTQAALPYRRNTGKDISLDALAARCEV
                     WDAPVPAGVAVLVAGIDVQDYRIEIEIVGWGRNEESWSIDYHVIDGEISHPDTQAALD
                     AYLAGAWHRADGRAFTLRAACIDSGGHHTDAVYNFAKARLGRKIWAIKGESARTGFTN
                     PVWPIKRPSSRSKKSFRPIIIGVNAGKDFVRSSLAKDKPGPGYMHFNIHTDLARFAQL
                     TSEEIVWEGHGAQRRRKWVPKAGRANEALDCRVYAYAALHGLMHMGLKLNREADAVGA
                     EFTAMAPSGAQDDQDTEGAQSLPQPGAEASAPTPPAPVSPAAAPAGPPRPAGASTPRR
                     RSGFSRGLA"
     gene            complement(31049..>31351)
                     /locus_tag="VFF98_14965"
     CDS             complement(31049..>31351)
                     /locus_tag="VFF98_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64985.1"
                     /translation="SFRSHVDAQKIKENALAARQLLTLRREAGELIEIERAETVYFDL
                     ARQQRDAWITWPTRVGPMIAADLGVDTARVIEVLTVHVQEQLEAIGEPEPDFTETD"
BASE COUNT         5404 a        10020 c        10537 g         5390 t
ORIGIN      
        1 cgggctgcgc ggcagcacag cagcgagcgc ggtgagctac cggcacaccg atcagccggt
       61 ctggcgcggc agctttgacg tctgcgacct gcgcgcgcaa gtcggccggc tgtttggcga
      121 tggcagcacg caacagggcc atgcggtgat caccgcgatc gtcgagacgg ccatcgcctg
      181 ggcggacaag gtgaaggaag agggcaaggc ccaccagctg accaccaatg tcatcaagac
      241 gctcgagcgg accttgcgca gcttcatgga ttttcgcacc ggcgcctgcg aggcgtccta
      301 cgatctgatc agcaaggcgg cctatttcgg ccgcatcacg gtcatccgcc acatgaagat
      361 cctgcgcgac ctcaaatggt tcgattgggt ccgccgcaca gagcccaccg gcaacgggcc
      421 caaggaaggg ccgcgggtca agcaggcggc caatgcctat ttcttcgaga tcagccgcct
      481 gcccgtggcc gcgcagatcc atctgcgcca gatcctcaag cggcgcggta tccggctgga
      541 cgagcacccc gagcgccagg gatcgggccc ggtgcccaac cgcatccagc gcctggccag
      601 ccgcgtctcg caggcgctga gcggcgccag gggcagccgc cgcagtccgg cagagaaggc
      661 agctctcatc gatgaggccg ccttcgtgcg cgcggaaatg gcatttttcg gcgacatccc
      721 gacggatcgc tgggcggcgc tacgccaccc gcaggatccg gccgcacagg cagcctacaa
      781 cgcccgcctc ggcatcgatc tttgtgcgac tgcgagtacc gggtcatcac ctgaatcccc
      841 cctgcagaac ggaaaggata aggaattcgg cagcagatag ctgccgaatg cgttgctgaa
      901 cgataaagcg ccacccgaca ggccaccgaa acaggagaaa acagcgccgg cggcaccggc
      961 gggcggggcg gcttgcgcca ccccggggct cggcgagggc cgaaaccgca agatccgggc
     1021 caagccgcca ccgccccgcg ggcggaaagg gcgcggccga tcaggctgca agattggtga
     1081 tgatcacctc gcccacgcgc tgggcaccgc catgggccgc cgcgatcgtc caggtcgtct
     1141 ccaccgggat gatgtgaaag gcgccgaagc attcccgcac ctcgggcaga tcattgaggc
     1201 tcaacaggaa ccggccgcgc gcgccgcgca gcagatcggc cagccgcgcg aaatccgccc
     1261 gactgaaaac ggccgcgcca tagtcggttt cgcagcccca atagggcgga tcgagataga
     1321 acagcatgcc cggccggtcg tagcgctcga tgaagcggtc gaagggcagc tgctcgatca
     1381 ccaccccggc cagccgatca tggatatcgg ccagcagcgg ctcgatcttg gtgatgttga
     1441 accgggcgcc agcgcgtttg tcgacgccaa aattgcgccc ggtcaccttg ccgccaaagg
     1501 ccagccgctg gagatagagg aaacgggcgg cgcgctgcag gtcggtcagc ctctcggacg
     1561 ggagggccag caggcgatcg aattcggcgc ggcttgccac gcgaaagcgc agcatgtcga
     1621 tgaaataggg gtaatgctct tgcagcacac ggaagaaggt ggcgacatcg ccgctgatgt
     1681 cgttgatcac ttccacgccc ggcctggtcg agcggcgcag gaatatgccg cccatgccca
     1741 caaagggctc ggcatagcca tcatggtctg cggcatcgat gagagagcag atgcgacggg
     1801 caagattgcg cttgccgccc tgataaccag cagcaggcga gacggggcgg acagaatcaa
     1861 gcgttgattt catacgtaaa tacacctagc aggccccacc cgttgcgcgg gtgcggggcg
     1921 gccggttggc cagctacgtc gtggcgaggt ttgctctcgt cggtggccgg gttggcgccc
     1981 ggtcaccccc gctcattatg agcgcgggaa tatttggcat gcgcaatgta gctattgact
     2041 acatatgtag ctgtaggcta cataatacca atgaagccga tcacttacac ccgcgccgca
     2101 ctgaagacgc tccgccgcat tccggcgaac acctcggcgc tgatcactgg caagatcgag
     2161 caattcgcat cagatccagc ctcgctggcg aacaatgcga aggcgctgac gggccgccag
     2221 ggcatccggc tgcgggtggg tgattggcgg gtgatcatgg atgatggcgt ggtgctggcg
     2281 gtattggaaa tcggcccgcg cggcggcatc tacgactgaa agaggagttt cccatgggcg
     2341 agatgatttc gatcccggtg gaagaatatc ggctgctgcg cagcgcggcg caggaactgg
     2401 aagaactgcg cacgtatgac ggcgccaagg cggcgctggc cgccggcgag gaagagttga
     2461 tccccgctga atatgtgaag cgcatgctgg cgggcgaaag cccactgcgc gtgtggcgcg
     2521 attatcgcgg cctcacccaa ctgcagctga gcgaagccgc gggcgtcaac cgtgtgcaga
     2581 ttgccgagat cgaggctgcc cgcaaggtcg gctcgatcga gacgatcaag aagctggcag
     2641 tggccctggg catcacgata gatgatctcg tctgatcagc cggccagcag cgcgccccag
     2701 tgctcaaaca gcgcgcgccg gcgggccagt tgctgggcgc ggttataggc ggcctccacc
     2761 ttgtccttgg ggctgtgggc cagcgcacgg tcgatcgcgg cgcgctcatc gggcagaacc
     2821 tcgttgagga tggtggagaa gctggcgcgc cagccatggg gcacatggcg cccggcaaag
     2881 ccggcccggg catagagcgc gccgatggca ttctcgccca gcgggcggcc cggcgaagcg
     2941 cccgggaaaa cgagggtttc cgctgccagt ggactagcac gcccgtcctg cccctgctgg
     3001 tgggccacct gccgcaacac ggccaccgcc ggagccgaga gcggcaccag atggtcgaag
     3061 cgctgctcgc ccttcttggc ccgggccagc ttcatgcgcg ccgccggaat gcgccagagg
     3121 ggcgcagggc cgcccagatc ctccacctcg ccccaccgca tgccgcgcac cgcctcgagg
     3181 cgcaccgccg tgagcgcaag gaagcgagag gccagcgcca cgatcggccg gccggccagc
     3241 gcattgcagg cggccagcag cgcgcggcag gcctccaccg tgcccagcga cggctgcggc
     3301 cggggcgggc ggcccttacg catggccgcg cccaggcggg cggccggatc gctgtcgatc
     3361 agcccctcgg ccacgccata ggcatagatc atcgacaggc gctggcgcag gcggcgcgcg
     3421 gtctcgatcc gtcccctgcc ctccaccgcg cgcagcgcat ccagcaattc cggcgcggca
     3481 atatccgccg ccggccggcc gcccagcgcg gggaagatat cgcgctcgag gctggccagc
     3541 acatctgccg catgggcggg tgaccattgt tcctgctggt gggcatgcca gctgcgagcg
     3601 agctgctcga gcgtgttgac ctgaaaggca acgctggccg gatctgcgcc ggcggcaacc
     3661 cgcgcccgcg cggtgtcgcg ccgggcccgc gcctcgggca gcgagatctc gggatagcgg
     3721 ccgatcgaca gcagccgctc gcgcccaccc cggcggtatt tccagcgcca ggcgcgcagg
     3781 cccgtgggcg ccacaaagag gaacagaccg ccgctgtcga acagcttata ggcgcgcgcc
     3841 tgcggcgccg cggctttcac cgctgcgaga gtgagcattg cgaaatctct tgctgtcagc
     3901 aggcgccaga caaacctgct tggtgttagt gagatggctt gtgattgaca tcaaagcgct
     3961 gagcgcggcg gatacaccct ccgccgcgct aaaattacga tatatttttc agtcaattaa
     4021 aatggggttt cggacgcgat acccctcatt ttcagggatc ataccccact ttgtatacgc
     4081 cgctcttttc gcctacgagc ccttcgcttg ctatcaacct tgactgccgc gggatcgcgc
     4141 ccagaaagta cgcgcgtacg tgcagcaaaa tggagttctc ttgcgttctc cagagatatt
     4201 gccggatatc gaccaatcgt gagcaatttt tctcgaccgt caaatatgaa tttatatctc
     4261 catacttttc gtccagtggg caagacatgc aaatataggc catttccgtc gaatatttta
     4321 tatggcctga cttgttgaac agcagatctg atttcttcgt cagaaagaaa tcctgaaaga
     4381 tgttcaatat aacccccatc acggggaagc catcttcgtc caatatttac aagccctggc
     4441 acccaccggc aattttcggg tccataattt ccatcaacat cacgcctgtc gagttgcaaa
     4501 tcatcccgat atccattatc cgccgcccat gcggaaaagg caggaaatga ctgccaactt
     4561 tggcaaatcg acacccctct cccgccatat tctgcatatt caggacgtgc ggcattttgg
     4621 caccgcgcct tcattccttt ccagatttgc tttagcctag tgctcactgg aaagttttta
     4681 catgaatcgt ggacggccgt tcaagccatt cactttctag attgccgtcg caaattatat
     4741 ctgcaaggcg tttttctaac tgatcgaaca agcaccgaag gttgaatcgg ttgatgatgg
     4801 catccttctc ctcgccatcg gactgacatg ccagctggct tagggcccgc agtgcatacc
     4861 gataggattc gagatcagcg ttttgcttgt catcgaggag atagaagcaa ctcttctcca
     4921 tcggtttctc agcgcgcgcc ttcacaatgg ggctccatcg ccaatagatc tggtgctaga
     4981 actgaattca gcctggaaca tgagcttcac ctcgtgcctg ggttaggagc cgggggaagt
     5041 tgcaccttgc cccggctctg cattttgggt tagacccaaa attatgagca cgtcaacaaa
     5101 aaaggttgaa cctaaaaagc gcggcaggcc ggcgacggga aaggatccgc tccttggctt
     5161 tcggtcgccg gcggaattga cggctcgtat cgatgcgtat gccgacagag agggtgtccc
     5221 ccgctcccaa gcaatccgct gcctcgtcga agaagggctt aaaaatatag cgtaatagga
     5281 gctaattggc gacaattgcg cttcctaacc cctccgccgc gcgtctgcgt gctatggcgc
     5341 aaatctgcca ctatcgaggc atcttcccaa aacgtgatac cccgagccat accccacctt
     5401 tgcgaccgcc acagcgcggc cggcatcaaa atccgttggg cctaacccgc cccctgcgag
     5461 atcagctgaa cgccatgatg ccagcagaaa tcctgttgag gacagcctgc cagtccgctg
     5521 ccaaaacagc ggcggggttg cggccggata cggcaaggtt ctccacccac ccacgataca
     5581 agcggaacgc gcgggcattt gcctcactgg cattgctgct attccagaac gggcccatgc
     5641 cccacagcag ctgcgaggca ctgtaattct gggtcgtggc agttgtgccg aggttggctg
     5701 tcgcgatgcc cgatgccgtc tggatgctca tcatggtcgg gccgccgttg gtgcggtaca
     5761 gggcgatctg ggccagggaa ccgaagtccc ggggaatgcc cacatacacg ctggcggatg
     5821 agccggagcc ggtctggcaa atgcccttca gcccgttatt ctgctgggtg atttcgacct
     5881 cggacagaat ggcgccgctg ttatagccag acgtgcatcc catgatggta gcgaccacgc
     5941 cgctatccgt gatccagttt gccagcgtcg gcagcttgaa atacccgcag atgatatact
     6001 gcgggttctg cgttccctgg cttgcataga gcgccgccat caccgatgct ggcgccgcta
     6061 cataggcccc attggccgcc accggcacag cggaaaaatc gaagccgccg ccggcgatcg
     6121 cgggcgctgt cgatgcgacg accgcggagc cgttgttgcc attgcccgaa aagtcgtaga
     6181 ccgtttgacc attggtcggt ggccatgcgg caggccgctg caactcattc cccgcatacc
     6241 cgccgggata gctataggga aacgccaggt cgaagccaaa caggaggccc ccattcgctc
     6301 cattgacgac aggatcacgt gtcaggagcg gcttgctggc gtcgttcgaa gagatgagcg
     6361 acttgatgat gttcacgccg taagtggcca tgatgggctc ctgcgattaa ttctgcacaa
     6421 gcgcgcgaat gaacccgtct tgcagggccc ttgcgccaaa ggtgatggaa ggatgaacgc
     6481 ccgtcgcctc aagcagcgga aaaacgccgc ttggcccata tacagtcgga tcggctccga
     6541 acagtttctg gaagtcgatc atcgcacagc tttgctcagc cgccagctgc tccatcttcg
     6601 cagcatagta cgacatcggc gcccaatagg gatatcctgc cgagatctcg gcaggcacaa
     6661 agagcaggat gtcgatatca gggatcgccg cgcggcactg cgagatgatc gtctgcacgt
     6721 cagtgtaata ctgggcggcg gttctgccag cattgcgatc attgatcggc gtaccgattg
     6781 agacgatgtt cggagcgaat gacgccaagg cggtagcaaa gctcgtcgca tcgccagcag
     6841 ctcgcccact gattttctgg ccggttgcag cacatttgaa aaactgcatc ccgttcacgg
     6901 tggcattatc gaactggacg ccgcagagat agggcgtgcc ggaaacgacg gtgatgacca
     6961 gcgaccaggt cgccgttgca ggcatgctgg cgagagccgt gatcgcaagg gcagcggcgc
     7021 tgctcacgtt gatcgttgtc gccgaaccgc cattccaggc ataactgaca gagccggtgc
     7081 catgccaaaa cagtttggcg atggtctgca ccgggttgct actgctcgcc gatgcgatgg
     7141 tataggtatc acccgccgtg gagctgtagg ccgagcagat atcggggctg tatgcgtctg
     7201 aaccgaagag atcggtccat gtgccagatc gaacgcacga atagagccac ggcgcaggcg
     7261 tgcccttgat gccgccgctg gcgccggtgg aggaaccgaa gccgacccat cccgggctgg
     7321 aatagccgat gctgttggcc gttccgccat tataggccgg gaaatcaacc gtcgaaagag
     7381 acgaggacca atagctgaac ccgtccgtgt agctatcgcc cagaccgagc caccggattt
     7441 gcgtaatagc gctgtcattg cagagccgcg ccctgatatt tctccgggtg tggcgcaggt
     7501 tatacgccat cttgatgggg atattcggcg agccataagg cgtaaggcca acgttcagcc
     7561 ggctcggaag atcgcttctc gagccagctg ccgaagcgac cacgccgccc atatcatccg
     7621 tgccgatgag gcggccatcg gcatcgacga tggcgccgtt ccgggtggat gcgtccgtcg
     7681 tctcccacca tgcggtggtt gcatggctga ccttggccat gatggcggcc ttttgcgcat
     7741 cggtcacacc accagcgttg atgacgcgac cagacgggtc aacaacaatg ccatccgcgg
     7801 cattcaggtt aagggtttcg aaataagaag gactggcctt gccggtgttg ttattggcag
     7861 cggtcgcttg gctgacggcc aggccctgcg cggtaatatc cgaagacata tcgctcccct
     7921 tactggttac tggccggcgt tgagttactg gccggcgttg agcatgaagg tgccgccggt
     7981 gccgccggtc agctcgagat agccgccccc cttgcaggtg atggcggtgg ccgcggtggc
     8041 accgaagctg gtctgcgtcg tgaaattgag atcgatgcag gtcatcgctg ccacggttgt
     8101 cccgccgtcc gggctccatt tgggggaata ggcagcagtg gcggccgcca ccgccgtgaa
     8161 ggtgacgctg ccataggccc gggcatcaaa ccaggtgccg gtggtgtagc tggcgggcgc
     8221 cgcatagagc ggctgcacat agggcgtgtt gttgacggac accgacaggc ggctggacgt
     8281 gtcgacagcc gcctgattgc cgcttggccc ggcaatcgtg ctgtagcagc caccgaccca
     8341 gcaggtgatg tagagcggat tggctgaggt gcccttctcg gcccccgagc tgtcgatgat
     8401 gaccgcgccg gtcgatggca cgctcgcgcc cccggcgttc ttgatctgcg gcagggtgac
     8461 ctgggcggcg gccggcgtgg cgatggcaag cgccagcagc gccgccagga agcggaggaa
     8521 aggcattgca ggaaactccg gatggaaagg ggcgcgcgat cagggcgcca gccagtcggc
     8581 aaggcttggc acggccgcca gcagcgctgc ggccagcgcc aggcgcagcc attggcccgt
     8641 agccatgatc agagggccat gatcaggccg ctggcccgca ggccttgtcg atcacgctgt
     8701 tgagcgcgat cgcctgcacc ttggtgccat cggtgtcggc atggctgagg gcgatggggc
     8761 gcagcacggc gcacagggga tcaatcgcgc cagtggcctt cggggtcggc atcaagggcg
     8821 gtgtgagcgc cgggactgct gcctgcgggg gcgggctcag cagattcgcg caggacgcga
     8881 gcatcagcat cggtgcgagc gcgggcagca tcagaaacga tcgcatgggc agcctcctgc
     8941 tgcgcgaggg cggtctgggc gcgggcattg ctgtccgcaa gggcgacggg cgtggggtct
     9001 ttcgagccga agaggcgccc aaggattgcc ttgagcgccg ccccgatgag ccccgaaaaa
     9061 agggcccaca tgtcaggcgg aaatacccag cagcttgagg atgctggtcg cccaggtgcc
     9121 ggccgtggtc gacttgaagt cgttgtagat ggtctgcacc aggctgcggc cgaggtcctc
     9181 cagatccgcc agctcagcct tcaggccctg ggcggtcagc agctgctgca gctgcggcag
     9241 cgtgtcgctc agcacctttt cgaacttctg gatgccggtg aggttggcat tgtcgatgtc
     9301 cgagatggcg ttggcgatgg cggtgccaac gggggtttcc ttcagcgccg cgacggcctt
     9361 ggtggcctcg ctcagcgcga aggacttgac cgaggccagt tcggtgctgg tcagaatggt
     9421 caggccaaag atcttgatag ccatgtggta tctcctggga aaaagccccg caaatgggct
     9481 ggcggagcgg cagcggttga aagggtcagg cagcgctcgc cggcgcgcga ttgcgcgcca
     9541 gtgccagcag gccgccgatg atcatcacgc ccacctcatg agcgccggca acgcccatgt
     9601 gttcggccgt catggtggcc agcccggtga tgacgatgcc cgccacaagc acgacgagat
     9661 cgctgaaatc cttatccatt gggtgtctcc ctgaggagcg gcccgatcag cggccgccca
     9721 tgatctgctg gagctggggc agcacgttgg tgagccggcc gccgacaggc tgatattgcg
     9781 gccccttcgc cgcgaagatc cgcacgcgca gatcggccgg ccagcgcgca tcgaagaaca
     9841 gatcacgctc acgcgtggcg cgggccagct ggcggccgtg atctgtccac tgcatgaagc
     9901 tggcgcgggc gctggtgggc cggcccgcca gcacatcctt cacccattgg gcggtgagca
     9961 gattgcccgg gccattgcgc caggcgaagg atagcgcgcc ggcgatctgg tgctcggcca
    10021 gcgcctggcc ggcgaaggcc ttggccagca ccggcacaaa gcccgtctgc agcagggcaa
    10081 tcgccgcctg aaggcacgct tcgagcggct gcggattatc gcgatattgg cgcacggcat
    10141 tgccgccgct ttccgcgatg cccaaggacc aggtccatat ccccttggga tcgagatagg
    10201 cagacaggcc aatggcctcc tcgctggcca cataggccag gatgcgcggg gtgagttgca
    10261 tgggtttatc ccggatgggg gcgcgccggc gggcggccgg cgcttatttc cagaggtgct
    10321 cgaggacgat agcgctgccg gccccaaaga cgcccgagat ccacatggcg accttttcga
    10381 tggtcgaccg gcgggcctcg cgcagcttca gctcggccac ttcgccggcc agcgcatcca
    10441 ccttggtgat cagcttttcg acagcggcct ccacgttcgt catgctctgc ttcaggccgc
    10501 ccagttcgag atagagttcg gtttcgggca tggggggatc cttcggcgcc gtgatgcggc
    10561 gcgcggtgcg ggggcgttgg gccatcggcc ttgtcctctg atcagctggt ggccggccag
    10621 ctggctgcat cgatctcggc ggtggtggca acggtgccgg cagagatcgc ggccgagaca
    10681 gtgttgagcg tagcccaggt ggcgccgatg aagctggcaa ccgcctgctg gatcgtctgg
    10741 atctgggcgg tggtgaccgt gacgtctccg ccggactggt agaaggtggt gctgcccgaa
    10801 gcctctgcct gcagcagcat caccgccaga tccgaaacgc ccgaggcgct ggtattcgcc
    10861 gcacaggcca caggcgccgc agtggtgccc acattgaccg tgatgccccc atcggcaacc
    10921 acctgccgcc gggcctgcgc ataggcgagc agctgggcgg cgctgggcgg cggattggca
    10981 atcgtgtgcg cttcgtcggc tgcgatcgcc gtccaggccg gatcaggcag cgtggggcct
    11041 gcccagcctg gcgcggccgc attggcctga tcctgcgctg agaggaaatg gacggcgccc
    11101 gcggcgtcct tgaaatattg gtcgatatag gcggtcatgg cttacctcag ctcgttccat
    11161 tggccgatcg agccattgct gatcgtcacc gagtaggact gcccgggctg gacgatgaag
    11221 gaggggatcg cggtcgccgt gcccgagctg tagtcgtagt tgaggttgtt cacgagcgtg
    11281 acaccgccga cggtgatcgt gatgttcggc gatccaccgc tggtatcggg gaagacaacc
    11341 tgcacgaaga ttgggcgctg ggtggtgttg taataggtgg tgccagatac ccggcttgcg
    11401 gtgacattct gccaggtctg gccgaagcct agcgattgcc aggccgccat cgcctcgccg
    11461 ccccggccct gcaccagcgc cggcgcggtg gcccaggtgc cggcgactgc ctcgctgatc
    11521 tgcacatagc ccaccagccg gaagggcgag gccgcgactg ccgccgccga atagaccgtg
    11581 cccgcgctgg tggcggcgct actgatcgcc gtgctgctga tcagcgtggt ctcatcgagg
    11641 ttcacgccgc cagcgatgtt gaccacggcc agcaccggcg ccccgccgtt gtagagcacc
    11701 agcagcgcca atgtggcctg ctggccggca atggtgccca gcgtggcgcc agacggcacc
    11761 gtgagcgcca aactggccgg cgcgcttgga ctgtaggcca cgggcgcgcc gctggtgagc
    11821 gttgcgctgc ggaaccacag cggcccggcg ggcgtccagc ccagcgtgag ggcattgctg
    11881 gcaacgccgg cgaaaaccgt ctggaagggc gcggagaaat tctgctgcag cgcctgcagt
    11941 acctgattgg tggccgtctt gctcggcgcc agcccggcaa aattgacgat cgccatcagc
    12001 tcttcctgga tcaggttgag ccagtcggcc gtgatcgtgg tgggcggcac gccagtgccg
    12061 gcattgccgc cggtgaaata gcccggggtg ccggcagccg ccggcgccgg cagcgcagcg
    12121 gccgccgtgg atgcatcgat gcggaacatg gggccctctg gtggattgcg aggtcagacg
    12181 aggaagatcg cgacatgcgc ggagatgatg gggctgagcg cggcgatcag cgcgctgctg
    12241 gccaatccgc tggtgttcgc ggtgatgtga atgcccagcg cagaggccca gttttctgca
    12301 tagaccgggc tgcctgctgt cgcgatgccg gcccggcagc gggcataggt ggtgatcgtg
    12361 atggcaaagc ccagcgtggc ggcaaagctg gtgaggaagg cgatgctctg gccgccattg
    12421 gcgatgaagc gcgagagcac ctgggcggag cgctgcgcgg tggtgaggcc cgagatggac
    12481 cccgaaaggc caagggtttc ctcccattcc ggcagcatgt cggcaaactg gcccggcaag
    12541 gatccatcca gcagctcttc tgccgccgca tcaagccgcg cccaacccgc gcccagggca
    12601 ttgagcaccg cttcctgccc cgagccctgt tcgacgcgcc agacgcgccc ttgcggcagc
    12661 agcgccttca gcgcgccggc ataatcgctg gtggatctgg ccatggctta aacgaaagtg
    12721 atggcgccgg ccgctggcag ggcaagcgcg ttggacgtga tgttgccggt gggccccggc
    12781 gtgacggtgc cggcgctggc cgcaacagcg gtgatgacaa agccggccgc gcccgagacc
    12841 gcggcgatcg ccgcctcgat gtcgctgagg tccagcgtgc cgcccggggc gcccttgctg
    12901 gtgacggcgc tggcaaaggc ggcctgcaca gcggcctgca cggtgctgcc ggcactggcg
    12961 aggcccgaga tggtgagcgt gatggtgttg ggcgttggcg tgaaggcata gaccagggct
    13021 tcggccggct cctcgctgaa aagagcgttg gccaccagca gctgatcgcc ggtggcggct
    13081 gtgtcgcgcg tctcgctggt ggccacgcca ttggtgccct ggggaaagcc gttttcggcc
    13141 gccctcacct catccatcat gaacagcaca acgaccgtgc ccggcccata ggcacgcgcc
    13201 acgcaccagg cgcgggtgac gcccgatacc gccagggccc agttctcgta atcggtgacc
    13261 gacccgcccg aaggctcttc ctgataggca accagcaccc ggctcttgaa atcgtcctgc
    13321 gtctcgacgt cggcgccgcc ggtgaaggcg gtggaaacgc tgccattggg cgtgatgccc
    13381 gacaggccga ccgacagata catggccgtg cccgcagcgg tgttgcccgc cgcgccggcc
    13441 gtggtggcag tggcctgcac gatcacgctg gtgccgctgg gcgaggccgc cgccgtggtg
    13501 gtgaaggtgg cgccatcggc ccgggtgatg gtggtgcccg cagggatcgg cgtggtggtg
    13561 cccgcggtgg ccgcaaaggt gatcgcgccc gaagcggcgg tcgcctcctt cagatagacg
    13621 ttcttcagcg ccccccaggc ggccagtgcc tcgccggtgg cggtgaaggg gttggactgc
    13681 ttggcgatat aatccagata gccatagcag ccattggcaa cgccggccga ggccgccccc
    13741 agcgcgcgca gattggcata gcgcagcagc gtgtccaatc cgggcaggtt gccatcgata
    13801 tcgccctgca cctggctgcg cagggaggag aggctgggcc gcgaataggg catttcagag
    13861 gaaatcccag agattggcaa aggcgagcgt gacagcggtg cccgatgccc gggtgatggt
    13921 gatggtgagc gcgagcgcgc tggtggacag ccattgcgag gagacctgca ccgtgctggc
    13981 gatgccgtct gtgaccagcc aggccagcgc gtcgcgcgcg tagccctccg cctgcgtcgc
    14041 aacgccagcg gtggccttca cccgcgagag cagccacaat ttcgagccga tatccccggc
    14101 ccaccagccg cgccggttgg ccgtgccatc ggggatcaca tcgtcgtcat cggcggccgc
    14161 atcagagaac aggctgatca gcacagcggt gacgagatct gccacccact ggtggctggt
    14221 ggaggcatcg agttcggcct ggacggtgag cccggtggcc cccaccagac tggcgcccag
    14281 acccacggcc tgcaacgccg cagcggcagg cacgaagatc cagtcgccgc tgcccgcagc
    14341 gttgctccag ccggtctgaa tatcggtcat agcgagtggt tggcgctgcc cgaggtgcca
    14401 gatccggtgg aaacgccggt gtggccgtgg gcgttgaagg tggagcggat ggtgctgagc
    14461 gccagcgcgg cgctgccatt gaggccggtg atatcgccgg caacggtgag gttaccggtc
    14521 aattccacat ccggcacgtc gatcgtcatc ttgctggcgt tctggatgat gagcggcagg
    14581 ccggcgcaat cgagcaccgg ccctgcggcg gtgaatttga ggatcacccc gcggacatcg
    14641 tacatggccg tgtcacccgg gctaaggccg gtggggcgcg aggcgcggtg gttggtgccg
    14701 atgatgatgg agcagacccg ctgaccgccc cgccgcaaca tcaccacctc gctgcccagc
    14761 ggaggaacgg aggtgaagcc gaaggagaag atccgccgca ctccatccag caccctggcc
    14821 atgaagccgc tgccgacagc accttcggtg atctgcagcc gttgtgtctc gccgctatca
    14881 tccacgccag tgacgttgcc ggcgccaacc atattggcga tggggcggga gacggtcatt
    14941 gattgccccc ctgcccagca tctgcgttga cgggcttcac atccgcagcg ctgaccggct
    15001 gcagcacaat gggctcaggc gtaaaagcga gagatggcat ggcgacaata tcagcggtgg
    15061 tgccggtctc attgttccgc ttgaaggtca cttccgagag gatcaatttt tctcccgtgg
    15121 tagcggccgg cagccatacg ggcatcagcg tgttaggcgc ccagagcttg cccgccttat
    15181 cgcgccagct gtcgaccgtg gccaacacca cattgctgcg acctgcccga cgggccattt
    15241 cccacttggc ccgcttttcc gtgaaatctt cagggtttgc ggccccatat tcctcgatca
    15301 gatacatcag ccgatgccgc ggaacattgg gatcgaaaac cgcagagaac aggtcggaac
    15361 cctgcacatc gccctctgta tcagcggcga tcccgacgca gatatattcg gaatagcgct
    15421 gatccatcga tcgctcgaca gaccaggcct gcacgttcac gccccatgaa ataccgctgg
    15481 cggcctgtgt gctaccggca ttggccaaca ccaggttgcc gaggctgtct tcatacgcga
    15541 gcaaagccgc gttgcgcgcg acccgctgga tgatatcggc agccgtctcg ccatagttca
    15601 gcggccagct gggcacatcc gggcccgggc tggcgccatt ggccaacacc acattgatcc
    15661 cgtaatattg ggccaagcgc tgggcgataa tcagggcatt gccaccgatc atctggtgcg
    15721 agggccattc cgccccgcaa tccaccaaat ccgacgtctt gccgcgcccg gtgatctcga
    15781 tccggtgcgt gttggcatcg ccataatccc gcacgcgatc gacatagccg gtgatcaccg
    15841 tatcggcccc cagtttcacc acacagggat caccggcctc aaccggcaac tggtcggtag
    15901 ggccattgct ggctgtcacc tggaatgtcg gcgggaagcc ctcggtgcgc agcgtcacct
    15961 cgatatcttc ccatccggcg atctcggtgc cgtcgacgat cagggtgaga tcatcggtag
    16021 cggccacggg cttgttggcc accaccacga tatcggggcc ggtgaggtca gtgctcatgg
    16081 gctgtctcag ctggccagcg cctggaaggt ggcgggcatg aaaagcgggt gcacgggatc
    16141 tgcctgggcg atcagctgat cggcccggct gctgtcggca tagatctgct gggccagcag
    16201 cagggccggc agcggctgcc cgaaggcgaa ggtggtgagc tgccccagcg tggcactggc
    16261 cgcgcgcagt gaggcggcga tctggttgcg gcaggcatcg agcgcggccc aggcgtcatc
    16321 ctcaccggca tcggccgctg ctaccgccag cgcatcgagg accggggcga tctgcgccag
    16381 gcgcgcggcc gcatcatcca ggctggacgg ctgatattgc gccaccacat cggtgagtgc
    16441 cgccgccgcc gcccgccaga tcatggtggt gatcacctgg gtggcgctgg tggaaagccc
    16501 ggtgaggctg gccgacagca cccccagcag gatgcggatg gcatcggccg gatccgcaca
    16561 gcaggccagc agcgcctcga tcagggccgt ggctgccgtg cccacatcgc tgatatcggt
    16621 gagatcagca gccgcagccg tgctggccag cgtgctggct gcggtgctca ccgcggcctg
    16681 ctctgccgag gcatcggtaa tcagatcatc gatcgtcgtg tcaggggtat agccggtggg
    16741 gttgctgccg gtgaggccgg cattggcacc ggccgaatag cggccatagg acgtgccgct
    16801 ggccgcaggc agcacggccg agatgcggaa cagcgcggtg gcatcctgcg cgaaggcgat
    16861 gatcttggta gaccagccgg caatggcgcc gacgatatcg gcccgcacgc cggccaggct
    16921 gagcacgcca gtggcctcgg ccaggatcgt cgaggtgctg atcgagccgc cggaaaccaa
    16981 tgtggcaacg gtggatgccg cgatcttgat cagcgccgtt ttcagcttgc tggcggcctt
    17041 ggtggtctgt gtggtcttgg tatagccgct ggcggtggtg gtgctggtgg tagaggtggc
    17101 tgccgccgcg gtggaagtgg gataggcctg ctggccgctg gcgatgaatt cgaggctgac
    17161 ctcggaatag ttggaggcct cgagcccttc accgatcgac cagcgctcga gcatcaccgt
    17221 gagcgagccc atggtgggat gcaccagcgt gcccgtgccc ttcttctcgc aggcgctgac
    17281 cagctgcgcg cgctgcaggg caatggctgc gccaccgaat gtcacgtcgc catcgacgac
    17341 gaagccgcgc agcacatagc gctcggcctg gcgccccata tcctcagccc agcccacctc
    17401 cttacccggg tactggtgga gcgcgacgcg ccggccgcca ttggtctcgg tgctggtgac
    17461 ggcgaaggaa atgccgcgcc agctggccgg tgcaaccggc gtgtaggtcc gctttgtcgc
    17521 cgaggaagag gcgcccgcct gcccctgcac cgcgctggac tttgccgcgc cgccggccgc
    17581 cttgccgctg gtgagcttgt tcaccgcctg cagcgccagc gtggaggcgg aggaggtcag
    17641 gctcatgggc gtctcaacaa aaaagccgcc cgggtggggc ggccaaattc aggttcaatg
    17701 gtgcggcggc agagcctcag ttgggtatcg actgactgac tgcgcccttg tcactacgtg
    17761 cggtgaagcg cgtgcccggc ggcagatttc gcgcttcgac cagcaaatgc acattggtag
    17821 gtgtgggctg catggccacg ccaccaatga cccggtgcac ataatcgcgg gtttcccgga
    17881 aagggatccg ttctgcccac tgcgcatcgc tgatatggcc tttgcgcgga tcgccattcg
    17941 tgcggatcca gttgtcgacg ttgccctcgc cggcattgta ggcagcagcg gccaaagtct
    18001 ggtcgccacg atattttttc gccagcatcc ccacatattt ctggccgagg tcgcgattat
    18061 aggcctcgtc tgtgcggaag cgccacggat cccagggaat gccggtgcgc cgggcaacca
    18121 tctgcgcggt gcttggcagc agctgcatca cgccaacggc ccccttgggg ctgacctgcc
    18181 cctgccgcga ttgctcgcca ttgcgctcga tcgaatcagg caggttgcca gtgggcacgg
    18241 gcgcggcctg cgcgccgccc tgcccatccg gcttgccgcc catgaggcgg tggatggtat
    18301 gaagagctgt ccaggccaga ggattggtgg cgagaaggaa gtgatcgacc gcatcgccaa
    18361 agcctttggc agcgtcgcga agagcgttca ctgccggctt gaaattgcca gacaccgcat
    18421 ctgcaaaggt gcgggccgcc gacaattcgc catccagcag gccctcggta gacgtggcgg
    18481 caaagtggcc gccctgagcc tctacccgat ccttgatctg gctgagccgg gtgttcttct
    18541 gctgcaggcg ctctgcatcc gacaaattct gatcagactg cacattggca tatctgccgg
    18601 catcggtcat ttggtccgaa agggccttgc caccctgggc aaagaccggg atcgcttcca
    18661 tcggaatgcc ccatgcgcgc gccagcgtgc gccggccgct ggaattctgg cgcttgatgg
    18721 catccgcaat ggccggcacc atggcatcag tattgaccga gccatccttg ttcatctgga
    18781 actggatgcc cgtgcgccgc agcatgtcgc gggcctgccc gttacgccca tatttcgcat
    18841 cgttgagggt ttcggaaagg cttcccatcg ccccctgcgc cgcgccctta tccatgccca
    18901 tctgctcggc agcggccatg aattcctgca gcgcttgctt gcccacgccc atggtggtgg
    18961 cgaagcggct gaccttggca gtttgctcgg cccaactggc gccgaacttc tgcaccgcaa
    19021 tcgtgcctat caccgcggcg gcaccgacag cagcgattgt gccggccacc gtgcccagtg
    19081 ccgcttccat cacgccggcg ccttcaccgg tttcgacaaa ggccgctttc agcgccccga
    19141 agccatcgcc agtgcggcgc gccatatcgc cgatgccacc cgagatagtc gttttgctgc
    19201 cgaaggccct ggccagcccc tgctcgacca tcttgaacga ggtatgcgtg cgctgggcaa
    19261 agcggcgcag attacgctcg cgggtgcggg tctgcttgtc gtcgtcgtta gccgcttgct
    19321 gggccgcctg gcgctgcgcc tgcgacaggc gtttacccag cttctcggca ttgctggcga
    19381 accgggagag gcgcttctct gccgccttga tccccgcctg ggtgtcatct gaggcgctga
    19441 tcggcagatt gagcccggaa atatcagtcg acatgatcag accattccag ccagtcgttc
    19501 aacatcgacc acggataggg gtaaagctcg ggtggcagct tcgcgaactg gcgccccagc
    19561 agcgccaggc gctgacgcca gtcgatcggc cttagcgcaa aaaacggccg agatactccg
    19621 atgcgcggcg gaccgggcga acgccaagct tgcgcacggc agcttccggc acccccgaga
    19681 tcagcgcgat cgccttcaca tcggcttccc agcccgtgaa cttgtcgagc tcgatcatct
    19741 cgccggcagt aggctcgcgc aggcgcagca aggggtagct ttcgcttccc agctccaccg
    19801 gcttggcaag cgggatttcc agcgcttcgg gcggatcgag gcgctttttc agcgcatcca
    19861 ccaacggcag gagagctgcc agcgcgttgg cttcattttc cgaaagatcg cactcagcaa
    19921 cccgcctgcc aaaagcggcc agctgatcgg caatagcggc cagaatgtct tcgccagagg
    19981 cggcttgttc ggttgcatca gccatatcag ttctccgtca cgctggccga ggcaaagcgg
    20041 atgctgaagg tgccatcctc ggtgttgacc tcggccggtt caccatcgcg ccaggcgttc
    20101 tgggcgatga tcaccttgcc gttgatcagg ctggccacca cggtgacgcc attggcgctg
    20161 ttcagcgttg ccatcgagac agcgccgctg tcgcgcccct gccaggcgat atggccggcg
    20221 gccggcttct cgctgtagcc atgcaccgca tcctgccccg tgagcgtttc gcgcgtggtg
    20281 gaactgacca gataggtgcc ctggccggcg acggcatagg tgacgccgtc gaccgtgatc
    20341 gaaagggttc ccgcgattgc agacatgggg gcctcctcag ctcaggcgga actgcgccag
    20401 catggcgaac tggcgcagct gatcgatcag cacggccggc cacagcacgt tgacgcggtt
    20461 ggggttggta gcatccttct cgacgacgag attggccgcg aagacggacg attgctggac
    20521 gaagccatcg gtctcgagcg tctggtaggc cgcgatgatg tcggcgcgga tggtggatgg
    20581 ggtgacgata ttgctgttgg ccggcaggcg cgtgccatcg gctgccagct tcacccgggc
    20641 gtatttcgtc tgcacccagc tcttcatgta gcgcagcaca tacatcagcg tgtagagcgt
    20701 ctcgacttcg agataggaat tgtcggccgc gccctggctg ttggtggtgt aggtggtgat
    20761 gatgttctcg atcatcacat tgccgctggc atccaccgtc caggtggcgc agccactgta
    20821 gagcagcgtg gtgttgcgca ccgtgagcgg gaagcggctt gcctgcaacg gcgccagcag
    20881 ccccggcacc gtgaggaact gcagcggcac cgccggatca ttgcgcaggc tttgcgcggc
    20941 agcgcccgcg aaggcagacg cccatgccca gctggggttc ggcccatcgt aaaagccgat
    21001 cgcggtcagg tgctgatcgt tgcgggcggc agtggccgcc gccaccgtgc cggcggtgcc
    21061 gcgcagtgcg atccagccat ggccatagag ctgctggagc gggttccagc ggccggttgc
    21121 atcgctgagg aaggttttga gcgcggccag cgcggtgctg tcggtgagcg acagcacgat
    21181 gaaatcgtag gtcttgtcca ccagggccgc cagtgcattg gtgatcgccg gattgctggc
    21241 gccgccagcc atggcgacaa tcgcgatgcc gacgccggcc ggcagcgcct cgccgccggc
    21301 aaccccgcga tagttcaccc gcacgtcgat gtcgttgccg gccaggcccg cattgcgcgc
    21361 ggtcaaggtg accacgccgg ccgcagcgct ggcggtgacg ggcaggccgc aggagatcat
    21421 cgtgctgatc gattgccccg aggaggcgtt gatatcggcc gcgatgttgg ccgcgatgac
    21481 gctggcggtg tcgccgctat tgaccggcac cgagaccaac acgccggcga catagagcgc
    21541 gatcgtgccg gcagcggtgg ccggcccggt gacggtgatc gtgccggtgg ccgccgcggc
    21601 cccggcggca tcgctgagcg gcagcgcatg gacctcgccc gcgctgtcgg cattgccata
    21661 atagccggcc atctgggcga ggatcgagcc aaagccggcg ccggcgatcg cttcgctgac
    21721 agaggccagg cgcacgggcg tgttggccgt gaatgtgccc gcgctcgtca tctggccgat
    21781 cagcagcgtg cgctgcacgg tagtggccgt gttggcgtag gacggatcga gttcggcgga
    21841 gaacagcggc acccgaaggt tcgacggggt gagcgtgaag gggacggtca tggccggtta
    21901 ctcccccgcc ccggcggctc cagccgcgct agtgcccgcg cttgcatccg cgctgatggc
    21961 cgggctggcg ccgggcgccg catcggccac ctcgggcacc tcatcatcct cggccacggc
    22021 cagatcgcca tcggcgacca ggcgggcgaa ggtgacatcg tggggatcga cgagaacgcc
    22081 gcgctcatcg atgacgcgac gggtcaccgg gtggcgcacg aggcgccccg gcacgctgac
    22141 aacgcgcatg gtggaattcc tatgggttga gggagatatc cgcgctgacc ggaacgtcgg
    22201 gcgcgtaatt ggtggccgag agcagcacgt cggcggcgtc atcggcagcg atggctgcga
    22261 agctgtcgga gtcctcgaag aactccaccg cgaaatccat gatgatggcg gccacgtggg
    22321 tgcggccctc ggaattgtag gccagttggc tgtcgatcga ggcgatctgc tggatccgca
    22381 tgaacagcgc gtaggagttg acgatggcca cctcgatctg ccgcttcagc gcccagcagg
    22441 cgccctctgc ggcggccgcg ccggcattgt cgggttccgc atagctctcc acctcgccca
    22501 tcacgcggat ggtggcggtg gtggtgtaca tcaccgtgcc gcgcccggcc gattgccggt
    22561 tctcgcgcag gatgcgcagc ttgatcaccg gcagctggcc gctttgcgcg ggccagtcgc
    22621 ccggcttgta gacgcgcgag ccggcggccg tggcgccgtc ggccgtcagg caggcgagga
    22681 aaagcgacag caggctgtcg gaggtgaccg gatcttgagg ggcggcccag ggcatcaggc
    22741 acctgtccac atcaggtggc acagcacatg gccatggcca tcggacagcg gattgctgac
    22801 gatgtaggtt ctgccgctgg cctgatcgat cacccgatcg ccctgctgcg gcacatagcc
    22861 gcccagcgcg gccacacgga tgcccaccac cggcttttgg acggtgatgg ccatcccatc
    22921 atcgcccggc acggtgaccg aggcctcctc atcgagcacg gcatcaggca gcgggatcgg
    22981 tgtcaggccg gccggcacga acacgatccc gctgccgaag atgcccatga cggggctgag
    23041 caccatgcta ttccagtcta tggccatggc ctgtcagccc gatccgaact gtcaggccgc
    23101 gcccgaccag agcacttccg gccgggtaca gatgaacagc gggtaggaat aggcttccac
    23161 gcgccaccag aacttgcggt cgcgatcgaa gatcgggatc agataccaat ccttgccagg
    23221 cgtgttcacc cattcgaagc tttcgcccgg ggccaatgcc tgccggaaga tacccggcgc
    23281 atcgacgggg aagaacttca ccttggtgtc gggcaccttg atggtgctgt tgtcgtccga
    23341 cccgcgatag ttgaaccagt ggatcccgcc gaattccatg gctgcgaagg cgtagccctg
    23401 gcgcagttcc tgggcggcct gccagttgta gtaggtcttc accacgtcga catgctgggt
    23461 gagcgcatcc cagaaggcat cgccgcacag ggcgaagatc ttggtctcgg tggtgaaggc
    23521 gcccttggcc gcgcgccaca tgccgcgcac caccttgttg cagatgccgc gcagggtgaa
    23581 ttcgacattg gcggtgagat tgaacgccac ctcggccggc tgcgcgacgc cgaattcatc
    23641 gaaccagttg tagagctggc tgccatcggc atcgagcagc acgccctgga tcgcgccgag
    23701 gcgcatgttt tcccaggtgt attgcaggcg cgggatgagg ccggtggggc cagccacgcg
    23761 gcgggcgact tcggcttcca cctgcatcat ctcggtttcg gtgccgaagg cccggatgtt
    23821 ctggacctcg tgcgagtaga tcgtgtcgcc ctgctccagg cgcggcactt cgaaatagcg
    23881 catcatgcgc ttttcggtgg tgcgatcctg cgagggcggc gcgccgcgct ggctggactg
    23941 gatgaggttc agcacgccat cgcggttttc gatgccgagg gccgtggtgc ggatgggata
    24001 cggctcgaaa atgcccagat cgcccagctg gctgggcaga taggggatcc gctcgaaagc
    24061 ggcggtcatc tggaacgtcg agaacggatc ctgatggaag atatcaagcg agaccatgaa
    24121 agggctccat ctgagggatg gcgggcgtct cgcgacgcgc gcagtgcggc ttgcccaagg
    24181 ccgaatgggc ttgtggcgct tcgatcgccg aagggatcag cgcgcgcgga tgccgttggc
    24241 gagcaggccc gcgaggccgg cctgctgctg ggcagcggtg gtgacattgg cgccccacac
    24301 cagttcgctg gcgttcacga ccgtggggcc gcgcaccacg ccagcggcct tctgggcggc
    24361 gttggtggtg tccttgcggc ccgagcccag gatcgccacg gcgttctgca ggccgttggt
    24421 ctgggtgggg tcgtagggcg cataggtgag cgtgccggca accgtgatca caaagccgtc
    24481 ggccggggca aaagcggtgg cgcctgcggt gatggtgaag ccgatgccgc cggcgctgaa
    24541 ggccacgccg accgtgccat gcccgatctc gacgcccgtg ggcgaggaga cgatgaagtg
    24601 cgtggcatcg tcgaattcga cgacataatt gccctgcgcg gcgccggtgc ccacggtgat
    24661 ggcgccgaac gtgccattgc cggtgttggt gcccagcgcc acctcggtgg ccgtgccggt
    24721 gagcaatgcg cccagcacgg tgccggcggt gatgatgcca tagccggcag caaggctgat
    24781 ctgctcgcgc gtgtagatgc cgtcggacgg ctcccacacg aggaagcccc aaccgtgcca
    24841 gagttcgctg atcggggtga caagggggtt gcccatgacg gtgttccttc agaaaagagg
    24901 gggccggccc gcttcggccg gccgggatgg atcagcggcg gcccttgccg atcacgccat
    24961 cgatggccgc gccccagcca cgctggacgg cggcgccgcg cggctcctgc ggggcagtgc
    25021 cgccgacgcc cgggttgcgg gcgctgcgat cagagcgagc ttcacgctgg ccattgtcgc
    25081 gctggcgacc ttccatcgag cgcgcggccc ggctgaccga gcgcaccgcg cggtgacggc
    25141 cgatcgggct ttccatctgg gtgagcgcga tctcgagttc gggcggattg cccatggcgc
    25201 tcgccaccgc ctcgagaacg gcgcagcagc gcacccgctc gcgccggcgg gcgctggcgg
    25261 cggccgagcg gccattcatc tcgcgctcgt cctcctcatc ctcgtcgtcc tcatcggctt
    25321 ccgcacggcg cgaacggcgc gaaccgcgag cgttggcgtc atcgtcaccc tcatcgcccc
    25381 cgtcgtcgcc cccatcgccg tcggcccggc gggcgcggcg ggcgccgcgc tggttgtcct
    25441 cggggtcggc gttgggatcg ccctcgtcct cgcccgggcc atccccgtcg gcgcggcgcg
    25501 aacggcgcga gcggcgggca tcgccatcgc cctcgcgctg cccgtcctcg tcctcgtcct
    25561 cgtcctcatc gtcttcctcg gcgcggcggc cctggcgatc atcgccctga tcacgctcat
    25621 cagcggcgcg acgcgaacga cggccgccaa agccggcgag atgcgcaaag cggctcgtgc
    25681 ccgccatcaa acggctggtc atgtgagtgt tttccttgtt gtgagtgcgt ctgtgggctt
    25741 ggcgcccgaa aggatggccg cctggccggc ctagcccagc tctgtcagca gggttctgaa
    25801 ggcttcgtcg ggcgccatca cggcatcggc gaggccgtaa tcgacaccat cggcgccgag
    25861 aaaggtgccg gcctgggtgt cgaaaacctt cttcctgggc agcgcgcgat tgcgcgcgac
    25921 ggtggcatcg aagagatcgc ccacggtctc cacatcggcc ttcagccgct tgaaggcctc
    25981 gttcgacagc ttgatcactt ccgaaccttc cgccttgagg tcgcccttgg tgatcagcgt
    26041 gacctcgacg ccggccattt tcagcgcttc ggcgaagctc acatgcatca tgatcacgcc
    26101 gatcgagccg gttccgccgg tgcgcggcac ggtgatgaac tcggccgcgc tggccagcgc
    26161 ataggctgcc gagaaggcat tctcgcccag gatggcccag atcggcttgg ccttgcgggc
    26221 acggtagatc gtgtcggcca gatcgaagca gccggctacc tcgccaccgg ggctgtcgac
    26281 atcaaggacg atggcccgca ccttggggtt ggccagcgca tcgagcaaca gggcgcggat
    26341 ccgatcatag ccgctgatgc cgaagagccc ggccagccac cacagatcgc ccacctgctg
    26401 caccagcgtg cccttgaccg ggatgatggc gacgccctct accacctgca gcgcgccatc
    26461 gacagcgccg ctagtatccc ggcccagcat ctccatatcg ctggccgatg ccagcttgag
    26521 caggcgcacg gccatttcgg cggcctgcgg ggccaaggcg accggcgtgt tgaacaggcg
    26581 cgtggcaagg gcaaaaggca gcatcaggcg ggcacccggg tagcagaggg acggccatgg
    26641 cgatgggcgc ggaaatctgc cgcgcgcgca tggccagagg atgggccaga agatgggcca
    26701 gaggatgggg cagcagccgc cgccgaggcg ggcgactgat ccgccatggc atcagctgcg
    26761 caagggggtg gttgcatgcg cgcctcgatc tccgcgctgt cctcacagac gcggatcacc
    26821 tcgccgccga tcaggtggat ggcggcacag ccatcggttt cgtcgagcca ggcgacggcg
    26881 gcggcagcga tccgcacgct gcgccggccg cccagccgcg ttgcatcgaa gaacatggtc
    26941 actcggcctc cggtgctgcg gccacggcat tggccgcccg atcggcgccc atccattcgg
    27001 gctggggcag gcccagctcg cgcatgcggt gatattcgcg ggcgcgctgg tcgaggttct
    27061 cctcccaatc cgcgccctgc tcggcacaga cctgctccag cgtggcaaag cccgcatcga
    27121 ggcccagcac cgcgccctgc cgctctgcca ccggatcaac ccagccccgc ggcgccccca
    27181 gccattggca gcgggcatag gcggtgcgcg cctcggcaaa ggcgggcgca ttgcgcggca
    27241 gcggcaccag gccgcgatcg tgcagctctt ccagccaggt gccatagacc ggcgtggagg
    27301 tgttgacgaa gaaatcggcc aggcggcggc ggaacatctt ctccgattcg acgatgccgg
    27361 cacgggccga ggaccagctg ctttcggaat aatcgttgtg cgtctgctcg gccgagaggc
    27421 ccagcacctg ccccatcgag cgcagcatct catgggtgaa gctggagaag ccgctgttgg
    27481 gccgcacggg ggcgaccgtc ttgatatcct cgccgggggc cagcgtggcg atcttggcgc
    27541 cgctgaccga gatatcgcgg tcgcggtgga aatctgtgcg caggttctga taccagcccc
    27601 aggccatttc gtcctgatcg tcgtcgtcga gcgcctggcg gaccatatcc agatcgaagg
    27661 gcgaggtgac atagaggccg aaggccgagg cgacggtggc cgccgacagc tcgacgttgt
    27721 aatatttggc cagcatgcgc aggcgcccca gcaccggcgc gaagatcgac atgccgcgat
    27781 gctgctggaa gcgatcgggg tcgtaatcgt ggaagacgcg ctgccacccg tccgggtcct
    27841 cgcgcgccac ccgctcccat tccatgctct cgacggcgtt ataccagtcg ttctggtggg
    27901 ccttgcggat gtgataggcg atggcgacat cgtcatcatc gacctcgacg ccgccgcgca
    27961 tgtagcgcgt gtctggcccc tggaaggggt tcgacaggcg atcagggtca acgccctgaa
    28021 agcaggtggc gaaagccgcg ccctgctcca gccgccgatc atcgcgccag tgggagagga
    28081 tgatgctttc cccgtccacc agcttgtgcc ccagcgcgag gcgaaacatc tgcgtcatcg
    28141 tgtggcggcg cgccagatcg ttgaagcggc gcagatcctg agaccagctg cgccagtgcg
    28201 cttccaccgc ctgccggtat acctccgccc attcggcatc gaagcccagc gcgcggtaat
    28261 ccggcttcac caccaggcgc agatgaccac cgatggtgga atccagcagg cgcccgatcg
    28321 cccccttggc ccaggcatca ttgcgataga tgtcgcgcgc gcgggcggtg acgcgatcgc
    28381 ggtagaggtt gatctcgcga tcgggcgaat tgatccacgg cacccagtcg gccgtttcag
    28441 ccgaggtgaa gtcctgcgcg tcgtagggaa accactgcgg cgcaggcccg ttcaaggcgc
    28501 gcatccggcc gcgcgggcca gcggcctgct cgcggatccg cgcaatctcg cccgccggga
    28561 tgaggtttcc gttgggatga tagaccctgg gcgtgccgct catcggaacc acaccctggc
    28621 cggccggcgc gcacggcgga tgatgcccag ctcctgctgg atctggcgga tcagcatgtt
    28681 gaggctggcc atatcggcct gccggtagct caccgacttc gcgccatcgc cctgggcata
    28741 gctggccgag gccacctgct ggccgctttg cagctggaac agcgccagct gggcgctggc
    28801 gagccactgt tgcagcgtgg cagggggaat gcccgccagc acggtgggat gggaaagcga
    28861 catgcgacag atcctgtgac ggcccggccc ggggcttcag gcaaggcgac ttcaggcaag
    28921 gccgcggctg aagccgctcc tgcgcctggg tgtggaagcg ccggccggcc ggggtgggcc
    28981 ggccggcgcg gccgccggtg aaacgggtgc cggcggcgtg ggcgcggatg cttccgcacc
    29041 cggttggggc aggctttgcg ccccctcggt atcctgatcg tcctgggccc cggagggcgc
    29101 catggcggtg aattcggcgc cgacggcgtc ggcctcccgg ttgagcttca gccccatgtg
    29161 catgaggccg tggagcgcgg cataggcata gacccggcaa tcgagcgcct cattggcccg
    29221 gccggccttg ggcacccact tgcgccggcg ctgggcgcca tgcccttccc acacgatctc
    29281 ttccgaggtg agctgggcaa agcgggccag atcggtgtgg atgttgaagt gcatgtagcc
    29341 cggcccgggc ttgtccttcg ccagcgagga gcggacgaaa tccttgccag cgttcacccc
    29401 gatgatgatc gggcggaacg acttcttgct gcgcgaactg ggccgcttga tcggccagac
    29461 cggattggtg aagccggtgc gtgcgctttc acccttgatc gcccagatct tgcgacccag
    29521 gcgcgccttg gcgaaattgt agacggcatc cgtgtggtgg ccgcccgaat cgatgcaggc
    29581 cgcgcgcagg gtgaatgccc ggccatcggc ccggtgccac gcgccggcca gataggcatc
    29641 gagcgcggcc tgtgtgtcgg ggtgcgaaat ttccccgtcg atcacgtgat agtcgatcga
    29701 ccagctttcc tcgttgcgcc cccagccaac gatctcgatc tcgatgcgat agtcctgcac
    29761 gtcgatgccg gcgacgagca cggcaacgcc ggcaggcacg ggcgcatccc acacctcgca
    29821 gcgcgcggcc agcgcatcga ggctgatgtc cttgccggtg ttgcgccggt agggcagcgc
    29881 cgcctgggtg ttccaccaga cctgcagctt gtcctcattg ccctgggcgg cgaggaactt
    29941 cttggcgatg gcgccgggat tgtccttcga ccaggggctg taaagcttgc tggcctggaa
    30001 gccggcgtgc tcattggaca ctgcccaggc gccgcaatgg gcgcatctgg cgcgatagac
    30061 gccccagcga ttggaggccc accaatccca gatctgcccc actggcgcgg catggccgcc
    30121 gcgccagcag ctggcatagg catcgagcgg cagctggcgc tcgccgcagc attcaaaggg
    30181 tcgcgtctgg tgccagcgga tggtggcgag cgcccgcagg cgttcggcat ccgaccattc
    30241 cttgttgcag ccctcgcaat tgatccgcgc ggtgtgcggc agatgggtgc gcccgccgtc
    30301 ggccttgtcc cagtggacgt gctcgaagaa atcgaggaac tggcggtgct ggcaatgcgg
    30361 gcaggcgatg ctggcgcggc gctgatccga ttccagccag ctcgcctcga tattgctctc
    30421 gcccttgacg gtgggcgagc agacgcggat cgacagccag ttggcgaagg acgccaggcg
    30481 ctcgtcgcca atgtcgatcg cataaccctc gcgggtgatc ggatatttgt cgatctcgtc
    30541 gtaaaggatg atgcgcagcg ggcggcgggc caggttatcc gggctgccgc cgccaaccag
    30601 ggcgaggaag ccgcccggga aggccttata ggtgatcgtc tcctcggcgc tgcgggtctt
    30661 gccggtgccg accagcttgc gcagggccgg cgttgccgag atgaacggcg ccacgcgctc
    30721 tttcgagaac tgctcggcgg catcgtcctt gggctggacg atcatcatcg ggcagggatc
    30781 gaggtcggcg tggaagccga aggtattctc gatcagcgag gtcttcagca gctgggtggc
    30841 caccatgcag ctgaggatgt gcacgccggg ttcggtgacc gcgagcagcg ggccgcgggc
    30901 gatctccaca tccgacgtgc gccaggcgcc ggccgagctg ccggtgccgc gcggcttgac
    30961 ccgcttggca tcggcccatt cgggcacgct gatgcggggc ggcgggcgaa agccgcgggc
    31021 gaaggcgcgc cgcagccggg tgagcccatc aatctgtttc ggtgaagtcc ggctcgggct
    31081 ctccgatcgc ctcgagctgt tcctggacat ggacggtaag aacctcgatc acccgggccg
    31141 tatcgacgcc aaggtcggcg gcgatcatcg gccccacccg cgtgggccag gtgatccatg
    31201 catcgcgctg ctggcgcgcg aggtcgaaat agacggtttc ggcgcgctcg atctcgatca
    31261 gctcgccggc ctcgcggcgc agggtgagca actgccgggc ggcgagcgcg ttttccttga
    31321 tcttctgggc atcgacgtgg gagcggaagg a
//
LOCUS       DASRYE010000291        11731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_229418,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000291 DASRYE010000000
VERSION     DASRYE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229418"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFF98_14970"
     CDS             complement(<1..442)
                     /locus_tag="VFF98_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64986.1"
                     /translation="MRRVATMDMGQGRFGGAISSEMRAQRRRQVSAASMAAGGPAAAF
                     DLPPLVDARERRRPRRAHGWWAQLARPGGVPALADLDLAALAGDAAHALVFAMPEGRA
                     LWAGSALAGLGEGALMADLAAGARAAAAAAPGPWLVGAAGPAGRR"
     gene            complement(533..1270)
                     /locus_tag="VFF98_14975"
     CDS             complement(533..1270)
                     /locus_tag="VFF98_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZU64987.1"
                     /translation="MVFSPSQGGLAGAALSALVMSGIVALRYLASSGGFALATRLRRP
                     GLHAGQGRQIRREIGWSLLSCGLFGVPAGIVAWGWRQHGWTVIYSDIHARPWWWLPAS
                     LAIYLLAHDSWFYWTHRWMHAPRLFRIMHAVHHASRPPTAWAAMSFHPTEALTGAVVI
                     PALVFVVPIHVGVLALVLTVMTVMGVTNHMGWEIFPRWLVRGPLGAWLITASHHQRHH
                     TDYRSNYGLYFRFWDKACGTDRGLAEA"
     gene            complement(1315..1809)
                     /locus_tag="VFF98_14980"
     CDS             complement(1315..1809)
                     /locus_tag="VFF98_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HZU64988.1"
                     /translation="MSAADSPSLASAPLVAADVARGITRLFARNDIWCLGEMPLRSGR
                     RADLMGVDAKGQVVIVEIKVSRADLLGDAKWTDYLDHCDRFYWGLAGGLDIACMGGEA
                     FLPERTGLIVADGYDAEILRPAALVPLAAARRKVEVERLARTALRRQAVAADSEIHRW
                     AGGS"
     gene            complement(1859..2248)
                     /locus_tag="VFF98_14985"
     CDS             complement(1859..2248)
                     /locus_tag="VFF98_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64989.1"
                     /translation="MTATNPERPFALPGTAAPAIALEARWAALRQAALAAVALAGPVA
                     HAQAAGEVEFPPALRTVTGARRGLLEQGLDDLVAVMEAGLTALLEVHARGGNATVPAS
                     ALWEEFVLARKALLALVPAVTLASRPG"
     gene            2392..2468
                     /locus_tag="VFF98_14990"
     tRNA            2392..2468
                     /locus_tag="VFF98_14990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2426..2428,aa:Arg,seq:ccg)
     gene            complement(2637..3251)
                     /locus_tag="VFF98_14995"
     CDS             complement(2637..3251)
                     /locus_tag="VFF98_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64990.1"
                     /translation="MGGYGSGRQGGRPTVDASRKIDLAWLLRTGRAIEGQRIAGVLHW
                     HREGVRCGSIRYEARMDEPGQERLELFFTCGTGAGREAIHQSIRLTFTEPHYGGKRWW
                     MICPFSGLRSGKLYLPDSRTRFASRKAWGLGYQSQRIAAGERPREALFRMQGRLGCPL
                     GVGNWPVRPKGMWQRTYDRHLDRYEELERRAAADLFGHNGREPA"
     gene            4059..4868
                     /locus_tag="VFF98_15000"
     CDS             4059..4868
                     /locus_tag="VFF98_15000"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HZU64991.1"
                     /translation="MRHSSLIYGGLAVVSAGAIYLGSAYWSAAGFESAAKAGDADKIS
                     NMADMASIKEGLKSQVSAALAKKSVSNGSNSDNAMTGLAALFVSAVADKMIDAFVTPD
                     GLAAIMKGGRPGHSDGTIKDDGTLTQSSSDFLDIDHFRVKVSKQGGGTTSLTFERRGI
                     ITWKLVRIALPADLLEDKPSPVTAPVAAPPQAQGPLLSLPAGQTHPVKDGEDWAQVIP
                     ASTRPADAEDCDRPVLEKQTASLDGGVCLSAGLPLDDLKRYHSGQAVGREA"
     gene            complement(5141..5719)
                     /locus_tag="VFF98_15005"
     CDS             complement(5141..5719)
                     /locus_tag="VFF98_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64992.1"
                     /translation="MIDGAMSAWGDGKPLNRFISGAWEVGGISPCDSVAATSRFITLA
                     REWLRERGYDMPWIWVQECGSRSGAHCHILMHVPPALDPLFRSKPRQWAGKVLGGSYV
                     PKVINTRRLLYASSSALMRSAYYAELMGRLHYMLKCTPAALEGPLGMNGASVAAWGQS
                     GLVYGKRAAVWQGTREYAEWARDMLPVPEQIC"
     gene            complement(6176..6361)
                     /locus_tag="VFF98_15010"
     CDS             complement(6176..6361)
                     /locus_tag="VFF98_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU64993.1"
                     /translation="MKLLSIKHFCEIYHVSRSTVYRLKAQGVLPFVYIGRAVRIRVED
                     AEAWANSLRGEVSNDNE"
     gene            complement(6766..7953)
                     /locus_tag="VFF98_15015"
     CDS             complement(6766..7953)
                     /locus_tag="VFF98_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZU64994.1"
                     /translation="MALDLSKVGERERLRVQREPHWQRLHAGCFIGYRPSQRGDIGTW
                     IARAYDEDANKYRLKSLGDFGALPGNARFAAAKKEAEAHAVLVEAGGEVRAKIETVGD
                     ACREYGKSNPEAEKRFGRYVYGTALAKVKLAKLRRRHVQEWRRQLESSPALVTRCKGD
                     EKAYRPRAASTVNRDMTALRAALSKVLSPGAPNSEAAWQEALKAVRNADRPRTLYLDR
                     EQRRKLLKAMAAEAEPFVRALCLMPLRSGAIAGLRVGDFDKRTKELTIGKDKAGELRR
                     ITVPDEFAVLLAEQAVNKLPSAPLFMRANGKPWDKSSWKLPIKAAVQAAGLPDGTSAY
                     TLRHSTITDLVATKLPLLTIAQISGTSAEMIERHYGHLISDAALEALSRLELLTPAGA
                     PPR"
     gene            complement(8233..10272)
                     /locus_tag="VFF98_15020"
     CDS             complement(8233..10272)
                     /locus_tag="VFF98_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU64995.1"
                     /translation="MATPAFAQDQQASFGLGEIVVSGKPAPDIEITSQTLSAKAIALF
                     GRVTLDDAVALAPGVSAGNSGGSRNERLIFVRGFNRFEVPLTIDGIRVYLPADNRLDF
                     ARFLTNDIAEVQIAKGYVSVLNGPDGMGGAINLVTARPTKPLEARLDAALDLGRQGEY
                     GGYALDAQIGTRHDKWYAQASFGRHFTDHWDLPGSYVSTTNQAAGHRDFSRSADWRVN
                     VKAGYTPNATDEYAISYTRQEGEKGAPLHITDPVSAQKFWSWPAWNTDSLYFLSTTAL
                     GEQATLKTRLYRNTFYNLLRAYDNRYENSQTLGRSFNSPYWDEALGGSAELALHPSAR
                     GRLSIAAQIRSDRHEEAQTSFPSGSTEPLQTDLENTYSLAAEYAYHITPALSITLGGG
                     YDWRDLKRAEDYGAPLGTSGASVLFNYPMRNSSAWSGQGRIDWAASDVTRLHVSLSSR
                     ARFPTIFERFSQKFGTSIPNPALQPERARQIELGGATRLGALSLEAAVFHALVTNAIV
                     SESVLGYACTASTTPGPCAQTVMTQSRNLARGRFYGVELSASAQLARGLSLGANYTWL
                     HRDLVDPGNAAFHPTDVPTHKAFVYADWAVLRRLHLIPSADIASSRWTVTSATPITYY
                     RTGAYVNAALKAEYDLRKGISLSASVRNLLDENYQLVDGFPEAGRSYQLAIRAAY"
     gene            10412..10954
                     /locus_tag="VFF98_15025"
     CDS             10412..10954
                     /locus_tag="VFF98_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU64996.1"
                     /translation="MAPARFLWLIAASLVPAIAPAFAPAFASTADMPAPPACPAPVAP
                     AGAYAPWAQPAPLAAGQAADGAPLLKPGSAARLSLAPTPQVRYALRPEKPGGSVSYGG
                     LVALEVASAGTYRIALGSAAWLDVIGKGGALPSIAHGHGPDCTGIRKLVDFTLEPGRY
                     LLQISANGTPALTVMALALP"
     gene            10951..>11731
                     /locus_tag="VFF98_15030"
     CDS             10951..>11731
                     /locus_tag="VFF98_15030"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZU64997.1"
                     /translation="MRGALAALAALLPVLAPAAAGQTPAKARIELGRRLFYDADLSRD
                     GTMACATCHEQRHGFADGNRTHPGVTDEPGRRNVPGLANVGRFTRLTWADPRQTALAR
                     QVAVPVLGTHPVEMGMAGMADAIPRRLGRDPCYAQMFAAAFPGSGRPISFAHAASALA
                     SFERQLVSRASPYDRGRLPAAARAGAALFARDCAACHKGPDFTDMTYHRLAPADPTAA
                     DQGLFEVTGRRRDRARFRTAPLRNLAVTAPYWHDGSAATLAD"
BASE COUNT         2162 a         3920 c         3573 g         2076 t
ORIGIN      
        1 cacgccgccc ggccgggcca gctgcgccca ccagccatgg gcccggcgcg gccgccgccg
       61 ctgccctggc gccagcggca agatcggcca tcagcgcccc ctcgcccagc cccgccagcg
      121 ccgatccggc ccacagcgcc cgcccctcgg gcatggcgaa caccagcgca tgggccgcat
      181 cgcccgccag cgcggccaga tcgagatcgg ccagcgccgg cacgccgccc ggccgggcca
      241 gctgcgccca ccagccatgg gcccggcgcg gccgccgccg ctcgcgcgca tccaccagcg
      301 gcggcagatc gaacgcagct gcgggcccgc cagcggccat gctggcggca gaaacctgcc
      361 ggcggcgctg cgcgcgcatt tcgctgctga ttgcaccgcc aaagcggccc tggcccatat
      421 ccatcgtcgc aacccgtcgc acgtgttatc cttggccggg cactgtgggc acggcgggta
      481 aatggcccat taacgattgg atccgttaac cgccgccacc gggcgccgcc aatcaggcct
      541 ccgccaggcc ccggtcggtg ccgcaggcct tgtcccagaa gcggaaatac aggccatagt
      601 tgctgcgata atccgtgtgg tggcgctggt ggtggctggc ggtgatcagc caggcgccca
      661 gcggcccgcg caccagccag cgcgggaaga tctcccagcc catgtggttg gtcaccccca
      721 tcaccgtcat caccgtgagc accagcgcca gcacgcccac atggatgggc accacaaaca
      781 ccagcgccgg gatcaccacc gcgcccgtca gtgcctcggt ggggtggaag ctcatggccg
      841 cccaggcggt gggcgggcgg ctggcatggt gcacggcatg catgatgcgg aacaggcgcg
      901 gggcatgcat ccagcggtgg gtccagtaga accagctgtc atgcgcgagg aggtagatcg
      961 ccagcgaggc cggcagccac caccaggggc gggcatggat atcgctgtag atcaccgtcc
     1021 agccatgctg gcgccagccc caggcgacaa tccccgccgg cacgccgaac aacccgcagg
     1081 agaggagcga ccagccgatc tcgcggcgga tctgccgccc ctgccccgca tgcaggcccg
     1141 gccggcgcag ccgcgtggcc agtgcaaagc cgccgctgct ggccagatag cgcagcgcga
     1201 caatcccgct catgacaaga gcggacagcg cggcgccggc aagcccaccc tggctgggag
     1261 aaaaaaccat ggccgcagcc tagcgcgcca ggcccagcgc gcaaatgggg cagatcaact
     1321 gccgccggcc cagcggtgaa tctcgctgtc ggctgccacc gcctgccggc gcagggccgt
     1381 gcgcgccagc cgctccacct ccaccttgcg gcgggccgcc gccagcggca ccagcgcggc
     1441 cgggcgcagg atctcggcgt catagccatc ggccacgatc agcccggtgc gttcgggcag
     1501 gaacgcctcg ccgcccatgc aggcgatatc cagcccgccg gccaggcccc agtagaaacg
     1561 gtcgcaatga tcgaggtaat cagtccattt ggcatcgccc agcagatccg cccgcgacac
     1621 cttgatctcc acgatcacca cctgcccctt ggcatccact cccatcagat ccgcccgccg
     1681 gccagagcgc agcggcattt cgcccaggca ccagatatcg ttgcgggcaa acagccgggt
     1741 gatcccccgg gccacatccg cggcaaccag gggggcagag gcaagcgagg gcgaatcggc
     1801 agcggacatg gctgccattt taggaacgaa tggcgaacat acaagccgcc tcagcgaatc
     1861 agcccggccg gctggccagc gtcacagccg gcaccagcgc cagcagcgcc ttgcgcgcca
     1921 gcacgaattc ctcccacagg gcgctggccg gcaccgtggc attgccaccg cgcgcatgca
     1981 cttcgagcag cgctgtcagc cccgcttcca tcaccgccac cagatcatcc agcccctgtt
     2041 caagcaggcc ccgccgggcg cccgtcaccg tgcgcaaagc cggcggaaac tccacctcgc
     2101 cggccgcctg ggcatgggcc acagggcccg ccaacgccac cgccgccagc gccgcctgcc
     2161 gcaacgccgc ccagcgcgcc tccagcgcga tggcaggggc ggcggtgccg ggcagggcaa
     2221 aggggcgttc cgggttggtc gcagtcatgg caggctatgt ctaaccggcg atacttgcca
     2281 aaaccctaag ccatccgccc gcagggatac ggggcccggc cggccccatg gcccccatgc
     2341 aaaaaagcca ctggcattca cgcgcgggcg ctgctaaccg ggcgcttcaa cgcatccgta
     2401 gctcagctgg atagagtact gccctccgaa ggcaggggtc acaggttcga atcctgtcgg
     2461 gtgcaccatt tttcaataac ttaggcaagt tggcagcact ccgtacggaa tgagtacgga
     2521 aaacatgtgc cagcgcccct gcaaagggcg gcccacgcgc gcgcgcgaag cgtaatctaa
     2581 attcccccac cgaaggccag ccgcgaatct tgaagccgcg ttcggggcag cacagatcag
     2641 gccggctccc tcccgttatg gccgaaaaga tccgctgccg ccctgcgctc caattcctcg
     2701 tagcggtcca aatgccgatc atatgtccgc tgccacatgc cctttgggcg aacaggccaa
     2761 ttgcccaccc ccaatggaca gcccagccgc ccctgcatac ggaacagggc ttcacgcggg
     2821 cgctcgcccg cagcgatccg ctgtgattga tagccaaggc cccatgcctt gcggctggcg
     2881 aagcgagtgc ggctgtcagg caaatagagc ttacccgacc gcagcccgct aaacgggcag
     2941 atcatccacc aacgcttgcc cccatagtgc ggctcggtga aagtcaggcg gatgctctgg
     3001 tgtatcgctt cacgcccggc gcctgtgccg catgtgaaaa acagttcaag ccgctcctgc
     3061 ccgggctcat ccatccgcgc ttcatagcgg atagagccac atcgcacgcc ctcacggtgc
     3121 caatgcagca ctcccgcgat gcgctgccct tctattgccc gcccggtgcg cagcagccac
     3181 gccagatcga ttttgcgcga tgcatcaacc gtgggcctgc ccccttgccg cccggaaccg
     3241 tagccaccca tggctgcata atctcccgcc aaattttggg aagacaatag cacaatagga
     3301 acaaaatggg aactttgatg ggcccagttg gtcgcactcg ggctatctgt tgccaatctg
     3361 ctttttggca agcaggcaca tgaagggatc tatcgccttc attcctgccg ttcaggggct
     3421 cttatcgcgt agcaggaaac tgtcgtgcat tcacagcgag tcctgcgctc ccaaacacct
     3481 tgatgatcgc ctgtgaacac caaaccggct tgatgtaggc gaaaccctgt tttgcgatca
     3541 tcaaatggtg tagctgtcga taggctatca tcaaggtcgg caagctactc acgcctgcca
     3601 atctcggtga tctgacggag acactcttgc ctgttgccta tcacgacggc cgcttccctc
     3661 ccgccacgct tgatctgggc gcactgtttc cgttggtcgg acctgccaat gccgccatcg
     3721 cacggtatga aggcgttctc tccggtattc ccaattggtg ccgccatcag ggcgagcgcc
     3781 ggcaacgttc gtgttcccgg aactactcaa cattgccgag ggctgagacg ctttttgatc
     3841 gatagcgccc caaaagccga cattccgcga tccgtacaag cacctcgata gccggctttt
     3901 gttcatttgg gattttacta ccttctcctt gaaaatgcga gaattactca aatccgtgga
     3961 aatgccgtag cttaaattct tgcctgcgct tgactgtagc tgacagataa tacctttgcg
     4021 atgtaataca actctgagat ctgcagagaa gggtagtaat gcgtcattcc tctttgatct
     4081 acggcggcct ggcagtggtt agtgcaggcg cgatctactt aggttcggcc tattggtctg
     4141 cagcgggttt cgagagcgca gccaaagccg gcgatgcgga taagatcagc aacatggcag
     4201 atatggcttc catcaaggaa gggctgaaat ctcaggtttc cgctgctcta gccaagaaga
     4261 gcgtgagcaa tggatcgaac tctgacaacg ccatgaccgg gctcgccgcc ctttttgtct
     4321 cggccgtcgc ggacaagatg atcgatgcct tcgttacgcc tgacgggctt gccgccatca
     4381 tgaaaggggg gcgcccaggc cattctgatg ggacgatcaa ggatgacggt accctcacgc
     4441 agagcagcag cgattttctg gatatcgacc acttcagggt caaggtgtcg aagcaaggtg
     4501 gaggcaccac ctcactgacc ttcgagcggc ggggcatcat cacctggaag ctcgttcgga
     4561 tcgcgctgcc agccgatctg ctcgaggaca agccttctcc ggtcacggcg ccagttgcgg
     4621 ctccgcctca ggcacaaggt ccgctcctct ccctgccggc cgggcagacc catccggtga
     4681 aggatggcga ggattgggcc caggtcattc ctgcaagcac tagaccggcc gatgcagaag
     4741 attgtgatcg cccggtactg gaaaagcaaa cggcgtcgct cgatgggggg gtatgtttat
     4801 cagcaggttt gccattggac gacctcaaac gatatcattc aggtcaggct gtcggacggg
     4861 aagcgtaagg aggtcactcc gggcaattcg ctagcggtga tccgcaacgg gccttatcgc
     4921 ggctacttgc tggtgcagaa gcataagtac aacctagggc ccgaaatcgg ctcatatgat
     4981 tcgacatggg ttatcaaacc tgatgggacg gaaatactcg aggttccggg cacgcgggac
     5041 ggtgaggacg cacgggtgca ggcatggctg caagcgaaag gctgggtcgc aaactgagcg
     5101 tacccttctc acctccagcc gcgaggatat tcctaaatca tcagcaaatc tgttctggca
     5161 cgggcagcat atcgcgcgcc cactctgcat attcgcgcgt gccctgccac acagcagccc
     5221 gtttgccata aacaagcccc gactgccccc aggccgccac gctggcgcca ttcatgccca
     5281 gcggcccctc cagagcggct ggcgtgcatt tcagcatata gtgcaagcgc cccatcaact
     5341 cggcatagta ggcgctgcgc atcaaggcgc tggatgatgc gtagagcagg cggcgcgtat
     5401 tgatcacttt gggaacatag ctgccgccca gcaccttgcc ggcccattgc cgaggcttgg
     5461 acctgaacaa tgggtccagc gcgggaggga catgcatcaa gatatgacaa tgcgcgccgc
     5521 tgcgtgatcc gcattcctga acccatatcc aaggcatgtc atagccgcgc tcgcgcaacc
     5581 attcacgggc cagggtgatg aaccggcttg tcgctgccac actatcgcac gggctaatgc
     5641 caccaacctc ccacgcgcca gagataaagc ggttaagagg cttgccgtct ccccatgcgc
     5701 tcatggcgcc atcgatcaac cgttgggctt ggcgctctgt cagacacagg cttttgcgat
     5761 ctttgcggtt tcgtgcccca ccccacggga gcagacgcac gcccctctgg cttgctgcga
     5821 cagtcaatcc gaactcctct ctatatagag cgctcgcaaa attatgggta tattcattta
     5881 atatcagcta tttatgaaaa tacgcgtgag gcctgcagac acaaaaccct gctagtttca
     5941 aagcgacaat ggggcacgtc ggggcaccag gcacacaaag gggcaccagg cacacaaaaa
     6001 ggcggcggga atcggcattg cctcaattcc cgccccctct tgccatgccg cttttgcaga
     6061 tgactgcacg gcattttccg gttagaaagc tacatcccgt tgcgaggagc gatcagggtg
     6121 taagcgtcgg tgagggttcc agcctcatcg gcgctgctgc tttcatcgac agccattatt
     6181 cgttatcgtt gctaacctcg cccctcagcg agttggccca cgcctctgca tcttccacgc
     6241 ggatacgcac agcgcggccg atatagacaa acggaagcac accctgggcc ttgaggcgat
     6301 agaccgtgct gcgcgataca tggtagattt cgcaaaagtg cttgatcgag agaagcttca
     6361 tgtattgata atctccgttt tgcagtaatc ttcgccaaca cggcgaagga ccaaaacgta
     6421 catatgcgca cgaaagcctt atcgacaggg cataccatcc ccatcgaata attctcttga
     6481 tgcaccagat aatttctgta cgtttactgc gattccctta atactctggg cacccctttg
     6541 cccgcaaggg tcgtggtcag ggcatatcca cagccaaccg ctgccctgca aatcgcagat
     6601 ttctgccttt tccgtatcaa gcaaagggcc cggaaaggat tgcccccatc cgcttggggt
     6661 tccagcacct tagtatcgga aaggcgtgca cgcccggctt agcgaatcgg caaagcatct
     6721 tccagccgca tgcccccccc tctaagcagc atcacctgcc cgtgatcagc gcggcggggc
     6781 tcctgccggc gttaagagct ccaacctgct cagtgcctca agcgccgcat cgctaatcaa
     6841 gtgcccgtaa tggcgttcga tcatttcggc gctggtgcct gatatttgcg cgatggtcag
     6901 caagggaagc tttgtcgcaa caaggtcggt gatcgtgcta tggcgcagcg tgtaggctga
     6961 agtgccatcc ggcaggccag ccgcttgcac cgctgccttg atgggcagct tccagctgga
     7021 tttgtcccac ggtttgccat tggcccgcat aaacaaaggc gcggaaggca gtttattaac
     7081 cgcctgctct gccaacaaca ccgcaaactc atcgggcaca gtgatccggc ggagttcgcc
     7141 agccttatcc ttccctatgg ttagttcttt cgtgcgcttg tcgaaatcac caacgcgcaa
     7201 cccggctatc gcgcccgacc gcaagggcat aaggcataaa gcacgaacaa agggctctgc
     7261 ctcggctgcc atggccttca acagcttgcg gcgctgctca cggtccagat aaagggtgcg
     7321 tggccgatca gcgttgcgca cagccttcaa cgcttcctgc caggcagcct cgctgttagg
     7381 cgcgcctggg gaaagcacct tggacaacgc agcccgtaaa gctgtcatat cccgattgac
     7441 ggtagaggcc gcacgcggcc gataggcttt ctcgtccccc ttgcagcggg tcaccagcgc
     7501 aggcgaggac tccagttgcc tgcgccactc ctgaacatga cgacggcgca gcttggccag
     7561 cttcaccttg gcaagggcgg tgccataaac atagcggcca aaacgcttct cggcttcggg
     7621 attgcttttc ccatattccc tgcaggcatc gccaacggtt tcgatcttgg cccgcacctc
     7681 gccgcccgcc tccaccagca cagcgtgcgc ctcagcttcc ttctttgccg ccgcaaacct
     7741 tgcattgccg ggcaatgcgc caaaatcacc aagggacttc agccggtatt tattggcgtc
     7801 ttcatcatag gcgcgagcga tccatgtgcc gatatcgccg cgttgcgaag gacgatagcc
     7861 aatgaagcac ccggcatgca gccgctgcca atgtggctcg cgctgcaccc gcaaccgctc
     7921 ccgctcaccc accttgctca ggtccaatgc catcgcgcct tgctctcgtc ctgtacggaa
     7981 cgtacggaat gagtacggaa tacatggggg gacaaacagg agcaagtgcg gacgatagat
     8041 attgatttat aatattttta atgtgcctcg gcatacctgt ttgctgcaaa tccccgccct
     8101 ccgaaggcag gggtcacagg ttcgaatcct gtcgggtgca ccattttctc cccgcttatg
     8161 cacgacgctt atgcacgtca gggcttgccg cttcaggtgg cggaggccgg tgccggcccc
     8221 cacccctttg cttcaatagg ccgcccggat cgccagctga tagctgcgcc cggcctcggg
     8281 gaagccgtcg accagctggt agttctcgtc gagcaggttg cgcaccgagg cgctgaggct
     8341 gatccccttg cgcagatcat attccgcctt cagcgcggca ttcacatagg cgccggtgcg
     8401 gtaataggtg atcggcgtgg cgctcgtcac tgtccagcgg ctggaggcga tgtcggcgct
     8461 ggggatgagg tgcaggcggc gcagcaccgc ccagtcggca tagacgaagg ccttgtgggt
     8521 gggcacgtcg gtcgggtgaa aggcggcatt gccggggtcc accagatcgc ggtgcagcca
     8581 ggtgtagttg gcgcccaggc tcagcccgcg ggccagctgc gcgctggcgc tcagctccac
     8641 gccatagaac cggccgcggg caaggttgcg cgattgcgtc atcaccgttt gcgcgcaggg
     8701 gcccggcgtg gtgctggcgg tgcaggcata gcccagcacg ctttccgaga cgatggcgtt
     8761 cgtcaccagc gcatggaaga ccgccgcttc cagcgagagc gcgcccagcc gcgtggcgcc
     8821 gcccagctcg atctggcggg cgcgttcggg ctgcagggcg gggttgggga tggaggtgcc
     8881 gaatttctgg ctgaagcgct cgaagatcgt cgggaagcgg gcgcgcgagg agaggctgac
     8941 atggaggcgg gtgacatcgc tggcggccca gtcgatgcgg ccctgcccgc tccaggcgct
     9001 gctgttgcgc atgggatagt tgaacagcac gctggccccc gatgtgccaa gcggggcgcc
     9061 ataatcctcc gcccgcttca gatcgcgcca gtcatagccg ccgcccaagg tgatgctgag
     9121 cgcgggcgtg atgtgatagg catattcggc ggccaggctg taggtgttct ccagatccgt
     9181 ctgcagcggc tcggtcgatc cgctggggaa gctggtctgc gcctcctcgt gccgatcaga
     9241 gcggatctgc gccgcgatgc tgaggcgccc gcgggctgaa gggtgcagcg ccagttcggc
     9301 cgaaccgccc agcgcctcgt cccaataggg gctgttgaac gagcggccca gcgtctggct
     9361 gttttcatag cgattgtcgt aggcgcgcag caggttgtag aaggtgttgc gatagagccg
     9421 ggtcttcagc gtggcctgtt cgcccagcgc cgtggtcgac aggaaataga ggctgtcggt
     9481 gttccaggcc ggccagctcc agaatttctg cgcagagacc ggatcggtga tgtgcagcgg
     9541 cgcgcccttc tcgccctcct gcctggtata gctgatggca tattcatcgg tggcattggg
     9601 ggtgtagccg gccttcacat tcacccgcca atcggcgctg cgcgagaaat cgcggtggcc
     9661 ggcggcctgg ttggtggtgc tcacatagct gccgggcaga tcccagtgat cggtgaaatg
     9721 gcggccgaag gaggcctggg cataccattt gtcgtgccgc gtgccgatct gcgcatcgag
     9781 agcatagccg ccatattcgc cctgccgccc cagatcgagc gcggcatcca gccgggcctc
     9841 cagcggcttc gtcgggcgcg cggtgacaag gttgatcgcc ccgcccatgc catccgggcc
     9901 attgagcacc gagacatagc ccttggcgat ctgcacctcg gcgatgtcgt tggtgaggaa
     9961 gcgggcgaaa tcgaggcggt tgtcggccgg caggtagacg cggatgccat cgatcgtcag
    10021 cggcacctcg aagcggttga agccgcgcac gaagatcagc cgctcgttgc ggctgccgcc
    10081 gctgttgccg gccgaaaccc ccggcgccag cgccaccgca tcatccagcg tgacgcggcc
    10141 gaacagggca atcgccttgg cgctgagggt ttggctggtg atctcgatat cgggcgccgg
    10201 cttgccgctg acgacaatct cgccgaggcc gaaactggct tgctgatctt gcgcgaaggc
    10261 aggcgtcgcc atgacgcccg ccatgacgaa tggcaggcat ttgctgaaat gcatcgttga
    10321 aattccctca tgtgggcact tgttgtgcct tgtgattgtg ggcatcatct atattcctgt
    10381 gaatatagat tgcaacgccg aggagatgtt gatggcgcct gcccgcttcc tgtggctgat
    10441 cgcggccagc ctggtgccgg ccattgcccc ggcctttgcc ccagcctttg ccagcaccgc
    10501 cgacatgccc gcgccgcccg cctgcccggc gccggttgcc cctgccggcg cctatgcccc
    10561 ctgggcccag cccgcgccgc tcgccgccgg ccaggccgcc gatggcgcgc ccctgctgaa
    10621 gccgggcagc gcggcccggc tcagcctcgc gcccacgccg caggtgcgct atgcgctgcg
    10681 cccggaaaag ccgggcggct cggtcagcta tggcgggctg gtggcgctgg aggtggccag
    10741 cgctggcacc tatcgcatcg cgctgggctc tgccgcctgg ctcgatgtga tcggcaaggg
    10801 gggcgcgctg ccgtccatcg cccatggcca cgggcccgat tgcaccggca tccgcaagct
    10861 ggtcgatttc acgctggagc cggggcgcta cctgctgcag atctcggcca atggcacgcc
    10921 cgcccttacc gtgatggcgc tggcgctgcc gtgaggggcg cgctggcggc gctggcggcc
    10981 ctcctgcccg tgctggcgcc cgccgctgcc gggcaaaccc ccgcgaaggc ccgcatcgaa
    11041 ctgggccgcc ggctgttcta cgacgccgac ctctcgcgcg atggcacgat ggcctgcgcc
    11101 acctgccacg aacagcgcca cggctttgcc gatggcaacc gcacccaccc gggcgtgacc
    11161 gacgagcccg gtcggcgcaa tgtgcccggc cttgccaatg ttggccgctt cacccggctg
    11221 acctgggccg atccacgcca gacagcgctg gcgcggcaag tggcggtgcc ggtgctgggc
    11281 acgcatccgg tggaaatggg catggccggc atggccgatg ccatcccccg gcggctgggg
    11341 cgcgatccgt gctatgcgca gatgtttgcc gccgccttcc ccggcagcgg caggccgatc
    11401 agctttgccc acgccgccag cgcccttgcc agcttcgagc gccagctcgt ctcgcgcgcc
    11461 agcccctatg atcgcggccg gctgcctgcc gcggcgcggg ccggcgcggc gctgttcgcc
    11521 agagactgcg ccgcctgcca caaggggccg gatttcaccg acatgaccta tcaccggctg
    11581 gccccggcag accccaccgc cgccgatcag ggcctgttcg aagtgaccgg gcgcaggcgc
    11641 gaccgggccc gcttccgcac cgcgcccttg cgcaatctgg ccgtcaccgc gccctattgg
    11701 cacgatggca gcgccgccac cctggccgat g
//
LOCUS       DASRYE010000292        14547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_169133,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000292 DASRYE010000000
VERSION     DASRYE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169133"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..164
                     /locus_tag="VFF98_15035"
     CDS             15..164
                     /locus_tag="VFF98_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZU64998.1"
                     /translation="MIRQIGAVLLAEPAIAEMDLNPVILHPQGVVALDALMRLHAPTA
                     TPIMA"
     gene            complement(220..1986)
                     /locus_tag="VFF98_15040"
     CDS             complement(220..1986)
                     /locus_tag="VFF98_15040"
                     /inference="COORDINATES: protein motif:HMM:NF004789.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU64999.1"
                     /translation="MPTPDTDLPATEWDLVVVGSGASGLCAAVTAAWHGLRVLVLEKA
                     PVLGGATAWSGGWMWIPGNPLARRAGIEEDPRAPRTYLAHTLGRHYNAELIDAFLDNG
                     PAMVSFLEGNTAVQFTAGNAIPDVYGHTPGAAAGGHQIIARAFDARTIPHLLPRLRKT
                     MRETAFFGMPIAAGPDLAAFLSLTRSWRSFLHVVKRLSRHALDMARHGRATHLVNGVA
                     LIARLAKSASDLGVTMATDMAVAGLESDGGKITGVRISTAQGTTTIRARRGVVLACGG
                     FPNDIARRQALFPRTPTGREHLALPPASCAGDGISLGEQAGGHLVTDLASPVAWAPVS
                     LLRHADGSSGHFPHIIDRGKPGIIGVLQTGKRFCNEADGYYDYTAAMVAQAPQGAEVA
                     SWLICDQRFQRRYGLGHSRPFPVPVGPWVRRGYLERAPSIAALARQCGIDPAALTATV
                     ERFNSFARQGEDPDFGRGGTAYNRRNGDPLHDGPNPCVAPIEKGPFYAVKVQPGCFAT
                     FSGLRTNGAAQVLDEKAAPIPGLYAVGADMASVMGGCYPSGGINLGPAMTFGFIAGRH
                     AAQAEAANAEPEAMTLDEHFAI"
     gene            complement(2095..3531)
                     /locus_tag="VFF98_15045"
     CDS             complement(2095..3531)
                     /locus_tag="VFF98_15045"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZU65000.1"
                     /translation="MLSLADTPPAIIAPAVQTAPAAAPKPAPGRPAAATDTTRQIASI
                     IVTAEQLDIPPSIKPYLLPNPATPIQPGVAGSYWPTPHLLGDWLGARHWLADRGITPS
                     INYVGEVSSNLSDGTSRKARYADQWAFGLKVATPNIFGGAPAMTQVTITRRSGEGINT
                     ANGVQTLQLPAEIFGRGDIWRLTQASYRASIGTLEVKLGRIPVGDDFASAGCEFQTLY
                     ACGATPGQISPDYWHNYPVAPWAIRFKQTFSKALFAQVGVYQVSPANIDTTNGFRLGL
                     DNTSGVLIPTEIDWTPKFRNGLAGIYKAGVWFTTANHADVVLNTRGQLLPIAGGTPLM
                     RGGMVGFYASLRQQLIKPRADGTGGLVLFANAAINDKRTTKLDRKFVLGFLASSPLPG
                     RRQDEIGFTIGTVHVNNRLTRAYEQLSSQSAGVEVPRAELAGELFYGISLLPGIIVRP
                     NIQLYHHPGGYADRRDVVFLGTKTVVNF"
     gene            complement(3639..5015)
                     /locus_tag="VFF98_15050"
     CDS             complement(3639..5015)
                     /locus_tag="VFF98_15050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65001.1"
                     /translation="MNAPAPSASRPASAGFAQEAGPGHHGMAAASSAALHAERWSALR
                     LLVLALCCMLNALDGMDIVIFSYIAPVLIANWHLSPGTMGIIFSASLWGMMIGCLFIA
                     PLADRFGRRRLMLCMLAIIGGAMIASGLARTVPQLLAARFAIGIGIGAILASMAALVS
                     EYAPANQAGLAVSIENAGYPLGAVATGLMTAKLLPIYGWQNILLGAGALSLLALAITA
                     ALLPESVDFLLSAQPRGALARANRIIARLGGAPVPALPPKPAAAGKFPVRAVFEQGRA
                     GGTFALWIGVLTAFMSLYFTISWFTRLAAQAGLALDNAIYLGAVLNLGAFVGTIAMGG
                     LGRILGERRVSTGFLVLAAFFLVLFGTMHWPFAGAMAIAFLLGTAIYGGFNSFYALAV
                     HFYPSAIRSTGIGWAMGIGRIGAALGPMLGGLLLNQGFSLASIMYVFALPLLIAACSA
                     PFVRLKRA"
     gene            5186..6010
                     /locus_tag="VFF98_15055"
     CDS             5186..6010
                     /locus_tag="VFF98_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU65002.1"
                     /translation="MAHKLSLAFLTLAPASPVEAIDIAEQCGFDHVGLRLLPAAAGEP
                     DYPLLHDRQCLSDVLARLADSPVTIADVEIARLGAEGDVERLLPLLDRAAALGARHVL
                     TAGDDPDPSRLTDTFARFCALAQERALTVDLEFMPWTAVKCARSAQAIVAAAGAANGG
                     ILVDALHFDRSATTLEDVRQLPPSRLHYAQLCDAPADFDPTDEGLIAVARGGRLFPGE
                     GGIDLTGLVRALPGDIPLSVEVINTGLFQGMSLLERGRRAMASARRVLMHAESAPA"
     gene            complement(6007..6810)
                     /locus_tag="VFF98_15060"
     CDS             complement(6007..6810)
                     /locus_tag="VFF98_15060"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65003.1"
                     /translation="MTLLLDGATRLHFIVGDPIAQVKSPGGVSQMLQDGGHNALVVPA
                     HVAPADLAAFVQGATLTRNVDGIIVTVPHKIAMTAQCASLSDRAAFLGAVNTLRRNAD
                     GTWHGDMFDGLGYVAALEEKGVSLAGRSVLLAGAGGAGSAIAHALVEAGVARLAIHEQ
                     DHARRDALIARLARLGKADVQAGSPDPAGFDIAINATPTGMRASDPLPFDIASLSAAH
                     IVGDVITQPLVTPLIEAARARGCLTVIGTDMFAKVRDLMVAYLLAHPAG"
     gene            6947..7765
                     /locus_tag="VFF98_15065"
     CDS             6947..7765
                     /locus_tag="VFF98_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU65004.1"
                     /translation="MLQRSLAILELLSAEPEGLALGVVAERLGLPRSATHRLLNELVH
                     YGYLRQPVDHGVYALTTKLVAMGLSFLGSSGIIDIAQPILDRLAQVSGEFVRLSILDG
                     DRLTWVARSQGSRAGLRYDPEMGAAAAFSCTASGHAILQTMTDDEALLVVGRQGFGKV
                     ADYGPNAPTTPTALLKALKAARKRGFSITIETFTPGMAAMAAPVRRRGQAPIGAISVA
                     GPHVRLTEARMVALGDELLRAAEELAATADASPLLRRSRSRAEPHALTRIDEAD"
     gene            complement(7827..8444)
                     /locus_tag="VFF98_15070"
     CDS             complement(7827..8444)
                     /locus_tag="VFF98_15070"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZU65005.1"
                     /translation="MQRYEIATLTTTLGAAAKAAPAIVDFAAGPDAKGQLLGLWATDI
                     GPLNQVIVLRGFDEAADLQQERERTLRSANPFGAAPWLVGLSLDSYAPFPFMPPVASG
                     TFGPIYEIRTYVLKHGGVEPTIAAWEAAMPRRSAYSPLLAAFYSLDGPPRITHIWPYA
                     DLAQRTEARTRSVVEGAWPPKGGPDWLTSDMQSAIALPLADSALR"
     gene            complement(8466..10181)
                     /locus_tag="VFF98_15075"
     CDS             complement(8466..10181)
                     /locus_tag="VFF98_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZU65006.1"
                     /translation="MSPPDSCVPAEEVDLLVFGAGAGGMTAALVASLEGLNVLLCEKT
                     GQVGGITATSGGTAWVPGTDLSIRAGVPDSTADAAAFLASVVGNRGGDALRQAFLDSG
                     PAAIRELDEQTDAKFVAASAHPDYLGNHPGAAFGGRALAPLPFDGRLLGRDFARIRPP
                     RPEFMGPTGMMVARNELDALLRPFASLAHFKAAAGLLLRQAADRLRYPRGTRLVMGNA
                     LVARLFYSLRQRKVPVRFDTALRDLIVEQGRVVGARVDGPAGPATLRARYGVVLATGG
                     IAHNPALRARLFPQSATRSLAPDGNTGDGVSAASRIGAPVDAGGDSPALWMPTSVLNK
                     PQGAEALWPHILLDRAKPGLIAVNAAGRRFVNESDSYHDFVMGMLRAHETAPSVPAHL
                     ICDAAFLQQYGLGLALPGAKGRRRLVKAGYLIEAPDLPTLAARIGVDAAALQRTVERH
                     NGFAQTGVDADFGRGTSPMNRFNGDPAHTPNPCMRPIATAPYYAVAVWPADLAASAGL
                     QGDVDGRVLDESGAPIAGLFACGNDLASIFRGTYPGPGTTIGPAMVFGWRIAKAAAKA
                     AHLPS"
     gene            complement(10168..11943)
                     /locus_tag="VFF98_15080"
     CDS             complement(10168..11943)
                     /locus_tag="VFF98_15080"
                     /inference="COORDINATES: protein motif:HMM:NF004789.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65007.1"
                     /translation="MPDLPVLHDCDVLIVGSGAAGMAAAVTAADLGLDVIVAEKAPLL
                     GGTTAISGGWLWIPHSPEARAAGFSEPREAPVRYLRALLGARYDDTLIQAFLDAGPQM
                     LAFFERHTQVHFQPGLTTPDFHGEIDQAATGGRSLVAEPYDGRQLGPLLAQLAPPLAE
                     TTLMGMAIAPGPDLRAFLTAFRSAASMLHVLRRLGRHLIDLLGHGRAMQLVNGNALTA
                     RLLRSAADRNVPLLTQTCVEALESDGRRVTGARVRTAGGVGLIRARRAVVLACGGFPH
                     DRQRQSLLLPHVAEGFAHHSAAPASNTGDGLRLAEALGALVDTGQVDPVAWAPVSLVP
                     GKNGQPTRFPHLVERGKPGLIAVTRAGRRFVNEGGSYHGYMRALFAATPAGQEPASWL
                     ICDRRFLRRYGLGAVKPFPVPIGRWLRNGYLLQGRSLRELAEACGIDPDGLAATIAAW
                     NAQARSGSDPAFGRGTTPYHRAQGDPDHRPNPCIAPIEAGPFYAVRIVPGSLGTFAGL
                     KTDAHARVLDREGAPIAGLYAAGNDMNSIMGGHYPGGGITLGPAMTFGWIAARHIAAQ
                     TQAQTQAQSQAQSEEAHANPPHVPA"
     gene            complement(12273..12866)
                     /locus_tag="VFF98_15085"
     CDS             complement(12273..12866)
                     /locus_tag="VFF98_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU65008.1"
                     /translation="MSRLTRSQSQEQTRQRLIDAARELFRRDGFAATSIDRIAEAAGY
                     SKGAVYSNFACKEEIFLAALQAEGAQSMAKLLEALHAAPDTATLIDLLADWADARAGS
                     GNWALTIMEYARQAENNAEALCQQEKILRACWQQLGACLLPRLPDLARRAGHDAQMVG
                     ALLFELAHAPALAHVDSPRPGALVRLVLGAAPAAEQG"
     gene            13047..13925
                     /locus_tag="VFF98_15090"
     CDS             13047..13925
                     /locus_tag="VFF98_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU65009.1"
                     /translation="MAWQGKRVVELAPSGQVDALAPTVSLANGVAMPAIGLGTWPMDD
                     AEAAGAVARAIGRGYRLIDTAQNYGNEAGVGAGVRASGVAREEIFLTTKFNRAWHSVA
                     GVREACEASLKRLGMDYIDLYLIHWPNPDQDRYLDAFHGLQRVAEAGLVRAIGVSNFK
                     PAHLARLLDAGLVPQVNQIQLDPYHPRADIVAIHQAHGIVTESWSPIGRGNALLADPA
                     ILRIAADHGRSPAQIVLRWQVQQGFIPTPKSADAGRQAANLALFDFALSAEEMAVLGG
                     LGRADPDMMDADVFGH"
     gene            complement(13929..>14547)
                     /locus_tag="VFF98_15095"
     CDS             complement(13929..>14547)
                     /locus_tag="VFF98_15095"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU65010.1"
                     /translation="VTLPIAANADSLATWLPDAIAAFASASGCFIELALDDEAHTAER
                     LRSGEVMAVVSSDPAPVPGCKTSPLGTLRYIACASPDFVARHFPQGPGPEALATAPHM
                     RFDRRDGLQASWAQRRGTGPLSGTTHWVPSTHAFLDLGLRGLGWAMQPEALARPHIAA
                     GRLVELVPDAPLDVPLYWTVARLHAGPLRALSEAVGRAARAQLRR"
BASE COUNT         2330 a         4682 c         5266 g         2269 t
ORIGIN      
        1 cggcgctggc aaggctgatc cgccagatcg gcgcggtgct gcttgccgaa cctgccattg
       61 ccgaaatgga tctcaacccc gttatcctgc atccgcaggg agtggttgcg ctggatgccc
      121 tgatgaggct gcacgcgcca accgccacac cgatcatggc ctgagggccg ggcggaaaag
      181 gggacggccc ggaactgtgt gcgcggccgc ttcccgccat cagatggcga agtgttcatc
      241 cagggtcatg gcttcgggct ctgcgttggc ggcttctgct tgcgcggcgt gccggccggc
      301 gatgaagccg aaggtcatgg ccgggccaag gttgatgccg cctgacgggt agcagccccc
      361 catcacgctc gccatatccg cgccgacggc atagaggccg gggatgggcg cggccttttc
      421 gtccagcacc tgggccgcgc cgttggtgcg caggcccgag aaggtggcaa agcagcccgg
      481 ctgcaccttg acggcataaa acggcccctt ctcgatcggg gcgacgcagg ggttggggcc
      541 atcatgcagc ggatcgccgt tcctgcggtt gtaggccgtg ccgccgcggc cgaaatcggg
      601 gtcttcgccc tggcgcgcaa agctgttgaa gcgctccacc gtggcggtca gcgcggcggg
      661 gtcgatgccg cattggcggg ccagcgcggc gatggagggc gcgcgttcga gatagccgcg
      721 gcgcacccag gggccaacgg gaaccggaaa cgggcgcgaa tggcccagcc catagcggcg
      781 ctggaagcgc tggtcgcaga tcagccagga agcgacttct gcgccttggg gcgcctgtgc
      841 caccatcgcg gcggtatagt cgtaatagcc gtccgcctcg ttgcagaacc gcttgcccgt
      901 ctgcagaacg ccgataatgc cgggcttgcc gcgatcgatg atatgcggga aatggccgct
      961 gctgccatcg gcgtggcgca gcagcgatac cggcgcccat gcgaccggcg aggccagatc
     1021 cgtgacgaga tggccgccgg cctgttcccc cagcgagatg ccatcgcccg cgcaggaggc
     1081 gggcggcagg gcgaggtgtt cccggccggt gggggtgcgc gggaagagcg cctggcgccg
     1141 ggcgatatcg ttgggaaacc cgccgcaggc aaggacgacg ccccggcggg ccctgatcgt
     1201 ggtggttccc tgtgcggtgc tgatccggac gccggtgatc ttgccaccat cgctttccag
     1261 cccggccacc gccatgtccg tggccatggt gacgcccaga tcgctggcgg atttggccag
     1321 gcgggcgatc agggccacgc cgttcaccag atgcgtggcc cggccatggc gcgccatgtc
     1381 gagcgcgtgg cgcgacagcc gtttgacgac atgcaggaac gatcgccacg atcgcgtcag
     1441 gctgagaaag gcggcaaggt ctggcccggc ggcgatgggc atgccgaaga aggcggtttc
     1501 ccgcatggtc ttgcgcaggc gcggcagcag atggggaatg gttctggcat cgaacgcccg
     1561 ggcgatgatc tggtggcccc cggccgccgc gccgggcgtg tggccataaa catcggggat
     1621 ggcgttgccg gcggtgaact gcacggcggt gttgccttcc agaaaggaga ccatcgccgg
     1681 gccattgtcg aggaaggcgt cgatcagttc ggcgttgtaa tggcgcccca gcgtgtgggc
     1741 gaggtatgtg cgcggcgcgc gggggtcttc ctcgatgccg gcgcgcctgg ccagcggatt
     1801 gcccggaatc cacatccacc cgcccgacca ggccgtggcc ccgcccagca cgggggcctt
     1861 ttccagcacc agcacgcgca gcccgtgcca ggcggcggtg accgcggcgc aaaggcctga
     1921 tgcgcccgat ccgaccacta ccaggtccca ttcggttgcg ggcaaatccg tgtcgggcgt
     1981 gggcatggtg gtctccggtg ggatgttatt atggaaatat gttctgaaaa ggaattatat
     2041 tccatttcat tccccgggcg tcaaggcggg cgcccggggg cgggaagggg gcgctcagaa
     2101 gttgaccacc gtcttggtgc ccagaaacac cacgtcgcgg cgatcggcat agccgccggg
     2161 atggtgatag agctggatgt tggggcgcac gatgatgccc ggcaaaaggg agatgccgta
     2221 gaacaattcc ccggccagct ccgcgcgggg cacttccacg cccgcgctct ggctgctcag
     2281 ctgctcatag gcccgcgtca gccggttgtt cacatggacg gtgccgatgg taaagccgat
     2341 ttcatcctgg cgccgccccg gcagcgggct ggaggccagg aagcccagca cgaatttgcg
     2401 atccagtttc gtcgtgcgct tgtcattgat ggcggcattg gcgaacagca ccaggccgcc
     2461 cgtgccgtct gcgcgcggtt tgatcagttg ctggcgcagg ctggcgtaaa agcccaccat
     2521 gccgccgcgc atgagcggcg ttcccccggc gatgggcagc agctggcccc gggtgttgag
     2581 cacgacatcg gcatggttgg cggtggtgaa ccagacgccg gccttgtaga tgccggccag
     2641 gccgttgcgg aatttcgggg tccagtcgat ttcggtgggg atcagcacgc cgctggtgtt
     2701 gtccaggccc agccggaagc cgttggtggt gtcgatgttg gcggggctga cctgatagac
     2761 gccgacctgc gcgaacaggg ccttgctgaa tgtctgcttg aagcggatgg cccagggcgc
     2821 aacgggatag ttgtgccagt aatcgggcga gatctgcccc ggggtggcgc cacaggcata
     2881 gagcgtctgg aattcgcagc cggcgctggc aaaatcatcg cccaccggaa tgcgccccag
     2941 cttcacttcc agcgtgccga tgctggcgcg ataggaggct tgcgtgaggc gccagatgtc
     3001 gccgcggccg aagatctcgg cgggcaattg cagggtctga accccgttgg cggtgttgat
     3061 cccctcgccg ctgcggcggg tgatggtgac ctgggtcatg gccggcgcgc cgccgaagat
     3121 gttgggcgtg gccaccttca ggccgaaggc ccattggtcg gcatagcggg ccttgcgcga
     3181 tgtgccatca ctgaggttgg agcttacctc gcccacatag ttgatgcttg gcgtgatccc
     3241 ccgatcggca agccagtgcc tggcccccag ccaatcgccc agcagatgcg gcgtgggcca
     3301 gtagctgccg gcaacgccgg gctggatcgg tgtggcgggg ttgggcagga gatagggctt
     3361 gatgctcggg ggaatgtcga gctgttcggc cgtgacgatg atggaggcga tctggcgggt
     3421 cgtgtcggtt gcggcggcgg gccgcccggg cgcgggcttc ggtgcggcgg caggggcagt
     3481 ctggacggcc ggggcgatga tcgcgggcgg ggtgtcggca agggaaagca tgggcgttct
     3541 ccggctcttc ggctctggcc gccgggcgca ccgctccctg cgcgcccggc ggggccgggt
     3601 gcgggggcat ggcgcggggg cggctggagg gggtgtgtct aggcgcgctt gaggcgcacg
     3661 aagggggcgc tgcaggcggc gatcagcagg ggcagggcaa agacatacat gatcgatgcc
     3721 agcgaaaacc cctggttgag cagcaggccg cccagcatcg ggccaagggc cgcgccgatc
     3781 cgcccgatcc ccatcgccca gccgatgccg gtgctgcgga tggccgaagg atagaagtgc
     3841 accgccagcg cgtagaagga attgaaccct ccatagatgg ctgtgccgag cagaaaggcg
     3901 atggccatgg cgccggcaaa cggccagtgc atggtgccga acaggaccag gaaaaacgcg
     3961 gcgaggacaa ggaaaccggt gctgacccgg cgctcgccca gaatgcgccc caacccgccc
     4021 atggcgatcg tgccgacaaa ggcgcccaga ttgagcacgg cgccgagata gatcgcgttg
     4081 tcgagcgcga ggccggcttg ggccgccagc ctggtgaacc aggaaatggt gaaatagagc
     4141 gacatgaagg cggtgagcac gccgatccac agcgcgaagg tgccgcccgc gcgcccctgt
     4201 tcgaacaccg ccctcaccgg gaatttgccg gccgcggcgg gcttgggggg cagcgcgggc
     4261 accggggcgc cgcccagcct ggcgatgatc cggttggcgc gggcaagggc gccgcgcggc
     4321 tgcgcgctca acaggaaatc gaccgattcg ggcagcaggg ccgccgttat cgccagcgcc
     4381 agcagcgaaa gcgcgcccgc gcccagcagg atgttctgcc agccatagat cggcagcagt
     4441 ttcgcggtca tgaggccggt tgccacagcg cccagcggat agcccgcatt ctcgatcgac
     4501 acggccagcc cggcctggtt ggccggcgca tattcggaca ccagcgcggc catgctggcc
     4561 aggatggcgc caatgccgat gccaatggca aagcgcgcgg ccagcagttg cggcaccgtg
     4621 cgcgccagcc cgctggcaat catcgcgccg ccgatgatcg ccagcatgca gagcatgagg
     4681 cggcggcggc cgaagcggtc tgccagcggc gcgatgaaca ggcagccgat catcatgccc
     4741 cacaggctgg cgctgaagat gatgcccatc gtgccggggc tgaggtgcca gttcgcgatc
     4801 aggaccggcg cgatatagga gaagatgacg atgtccatgc catcaagggc attgagcatg
     4861 cagcacagcg ccagcaccag caggcgcagg gcgctccacc gctcggcatg gagggcggcg
     4921 ctgctggcgg cggccatgcc atgatggccg ggcccggcct cctgcgcgaa accggcgctg
     4981 gcggggcgcg atgcggaagg ggcaggggcg ttcatggccg cttgcccatg gccgaacagg
     5041 ggtgtggacc tttcatcatg cgcctctcct tgaccgagcg gattatccgt ttcgctcttg
     5101 cctgccggct ctacaacaaa aatggactta gagtctatat aggaatatca ttccacaaat
     5161 tcgcatcgca tttcggagca cgctcttggc ccacaaactg tctcttgcct ttctcaccct
     5221 ggcgccggcc tcgccggtgg aagctatcga tatcgccgaa caatgcggtt tcgatcatgt
     5281 cgggctgcgg ctgttgccgg ccgctgcggg agagccggat tacccgctgc tgcatgatcg
     5341 ccagtgtctg tcggacgtgc tggcccgcct ggccgacagc ccggttacca tcgccgatgt
     5401 ggagatcgcc cggctgggcg cggagggcga tgtggagcgc cttctccccc tgcttgaccg
     5461 ggccgcggcg ctgggcgccc gccatgtgct gacggcgggc gatgatcccg atccctcgcg
     5521 ccttacagac acctttgccc ggttctgcgc gctggcgcag gagcgcgcgt tgacggtcga
     5581 tctggaattc atgccgtgga cggcagtgaa atgcgcgcgt tccgcacagg ccattgtagc
     5641 ggcggccggg gcggccaatg gcggcattct cgtcgatgcg ctgcatttcg accggtctgc
     5701 cactacgctg gaggatgtgc ggcagctgcc gccatcgcgc ctgcactatg cccagctgtg
     5761 cgatgccccc gccgatttcg accccaccga cgaagggctg atcgcggtgg cgcggggcgg
     5821 gcgcctgttc ccgggcgagg ggggcataga cctgacaggc ctcgttcgtg ccctgcccgg
     5881 tgacattccc ctgagcgtcg aggtgatcaa caccggcctg tttcagggca tgagcctgct
     5941 ggaacgcggc cggcgggcca tggcctctgc ccgccgcgtt ctgatgcatg ccgaaagcgc
     6001 cccggcctaa ccggccggat gggccagaag gtatgcgacc atcaggtcgc gtaccttcgc
     6061 gaacatgtcg gtgccgatga ccgtgaggca gccgcgcgcc cgcgcggcct cgatcagcgg
     6121 agtcaccagc ggctgggtga tgacatcgcc gacgatatgc gcggcgctca gcgatgcgat
     6181 gtcgaagggg aggggatcgc ttgcccgcat gccggtgggg gtggcgttga tcgctatgtc
     6241 aaagccggca gggtcggggc tgccggcctg cacatcggcc ttgccgagcc gggcgaggcg
     6301 cgcgatcagc gcgtcgcgcc ttgcgtggtc ctgctcatgg atggccagcc gggccacgcc
     6361 ggcctcgaca agggcatggg cgatggccga acccgcaccg ccagcccctg ccagcaaaac
     6421 gctgcgaccg gccagcgaca cccccttttc ctccagcgcg gcgacatagc ccagcccgtc
     6481 gaacatatcg ccgtgccatg tgccgtccgc attgcgccgc aaggtgttga ccgcgccgag
     6541 aaaagcggcc ctgtctgaca ggcttgcgca ctgggccgtc atggcgatct tgtgcggcac
     6601 ggtaacgatg atcccgtcca cattgcgggt gagggtggcg ccctgcacga aagcggccag
     6661 atcggcaggc gccacatggg ccggcacgac aagcgcattg tgcccgccat cctgcagcat
     6721 ctgcgaaacg ccgccggggg atttcacctg ggcaatggga tcgccaacga tgaaatggag
     6781 gcgggtggcg ccatcaagca agagggtcat tctgatctcc cggatgtaaa ttgagctgtt
     6841 ccactttgga atgctcttca aagaaagcag gggcagtgct agggaagggg cagcgcgaag
     6901 gtgcaaaacc gataggagac ttatgtcgca agtgccgctg tccggagtgc ttcagagaag
     6961 cctggccatc ctcgaactgc tgtcggccga gcccgagggg ctggcgctgg gcgtggtggc
     7021 cgagcgcctg gggttgccgc gcagcgccac gcaccggctg ctcaacgaac tcgtccatta
     7081 tggctatctg cgccagccgg tggatcatgg cgtctatgcg ctgaccacca agctggtggc
     7141 gatggggctg agctttctgg gctcttccgg catcatcgac attgcccagc cgatcctcga
     7201 ccggctggcg caggtgtcgg gcgaattcgt gcgcctctcc attctggatg gcgaccggct
     7261 gacctgggtt gcccggtcgc aaggttcgcg cgcgggattg cgctatgatc cggaaatggg
     7321 tgcggcggcc gccttttcct gcacggcatc gggccatgcc atcctgcaga ccatgaccga
     7381 tgacgaggcg ctgctggtgg tggggcggca gggtttcggc aaggtggctg attatgggcc
     7441 caatgcgccc accaccccca cggcgctgct caaggcgctg aaggccgcgc gcaagcgggg
     7501 cttcagcatc acgatcgaaa ccttcacgcc cggcatggcc gccatggcgg cgccggtccg
     7561 ccgtcggggg caggctccta tcggtgcgat tagtgtggca gggccgcatg tcaggctcac
     7621 ggaagcgagg atggtggcgc tgggcgatga attgctgcgc gccgcggagg aactggcggc
     7681 cacggccgat gcctcgccgc tgctgcggcg cagccgctcg cgcgccgagc cgcatgccct
     7741 gacgcggatc gacgaggccg actgaacggg gggcgcaagg ccgccggggc accacagcag
     7801 ggcgccggcg gccttgcccg catgcctcag cgcagggcag agtcggccag cggcagggcg
     7861 atggccgatt gcatgtcgct ggtcagccag tcgggcccgc ccttgggcgg ccatgcgcct
     7921 tcgacgacgg acctggtcct ggcttcggtg cgctgggcca gatcggcata gggccagatg
     7981 tgggtgatgc gcggcgggcc atccagcgaa tagaacgcgg ccagcagcgg ggaataggcc
     8041 gaccggcgcg gcatcgccgc ttcccatgcg gcgatcgtgg gctccacgcc gccatgcttc
     8101 agcacatagg tgcggatctc gtagatcggc ccgaatgtgc cgctggcgac cggcggcatg
     8161 aagggaaagg gggcatagct gtcgagcgac aggccgacca gccacggggc cgcgccaaag
     8221 ggattggcag agcgcagcgt gcgttcgcgt tcctgttgca gatcagccgc ctcgtcaaac
     8281 ccgcgcagga cgatgacctg gttcagcggc ccgatgtcgg ttgcccacag gcccagaagc
     8341 tggccctttg catcggggcc ggcggcaaaa tcgacgatgg ccggcgctgc cttggcggcg
     8401 gcccccagcg ttgtggtgag cgtggcgatt tcatagcgct gcatgagata tgtcctttga
     8461 acgcttcagg aagggaggtg ggcggctttg gcggcggcct tggcgatgcg ccagccaaac
     8521 accatggcag ggccaatggt ggtgccgggg ccgggatagg tgccgcggaa gatggaggcc
     8581 agatcgttgc cgcaggcgaa gaggcccgcg atcggcgcgc cgctttcatc aagcacccgg
     8641 ccatcgacat cgccctgcag cccggcgctg gcggccagat cggccggcca cactgccacc
     8701 gcataatagg gcgcggtggc gatcgggcgc atgcagggat tgggcgtgtg ggccggatcg
     8761 ccgttgaagc ggttcatcgg gctggtgccg cgcccgaaat ccgcgtccac gccggtttgg
     8821 gcaaatccat tgtggcgctc gacggtgcgc tgcaatgcgg cggcatccac gccgatgcgg
     8881 gcggccagcg tcggcaggtc gggcgcctcg atcagatagc cggccttcac cagccgccgg
     8941 cgccccttgg ctccgggcag ggcaaggcca aggccatatt gctgcaggaa ggccgcatcg
     9001 cagatcagat gggccggcac cgagggcgcc gtttcgtggg cgcgcagcat gcccatcacg
     9061 aaatcatggt aggaatcgct ttcgttgacg aagcggcggc cggcggcatt caccgcgatc
     9121 aggcccggct tggcgcgatc gagcaggatg tgcggccaga gcgcctctgc gccttgcggt
     9181 ttgttcagaa ccgatgtcgg catccacagc gccgggctgt ctccgcccgc atccaccggc
     9241 gcgccaatcc gggaggccgc gctcacgccg tcgccggtgt tgccatcggg cgccagggat
     9301 cgggtggcgc tttgggggaa caggcgtgcg cgcagggcag ggttgtgggc gatgccgccg
     9361 gtggccagca ccacgccata gcgcgcgcgc aaggtggccg gcccggcagg gccatccacc
     9421 cgcgcgccca ccacgcgccc ctgttcaacg atgagatcgc gcagcgcggt atcgaagcgg
     9481 accggaacct tgcgctggcg caggctgtag aaaagccggg cgaccagcgc attgcccatc
     9541 accagccggg tgccgcgcgg atagcgcagc cggtctgccg cctggcgcag cagcaggccc
     9601 gcggccgcct tgaaatgggc caggctggcg aacgggcgca gcagcgcatc cagttcgttg
     9661 cgcgccacca tcatgccggt ggggcccatg aactctggcc gtggcggccg gatgcgggcg
     9721 aaatcccggc ccagcagccg cccgtcgaaa ggcagcggtg ccagcgcccg cccgccaaag
     9781 gcagcgcccg ggtggttgcc cagataatcg gggtgcgcgc tggccgccac gaatttggca
     9841 tcggtctgct cgtccagctc gcggatcgcc gcggggccgg aatccagaaa ggcctggcgc
     9901 agggcatcgc ccccgcgatt gcccaccaca gaggccagga aggctgccgc atcggcggtg
     9961 gaatcgggca cgcccgcgcg gatgctcaga tccgtgcccg gcacccaggc ggttccgccc
    10021 gatgtggcgg tgatgccgcc cacttgcccg gtcttctcgc acaggaggac gttcagccct
    10081 tccagcgagg cgacgagcgc ggccgtcatc ccgccggcgc cggcgccaaa gaccaggaga
    10141 tccacctctt cggcgggcac gcagctgtca ggcggggaca tggggcgggt ttgcatgggc
    10201 ttcctctgat tgtgcctgtg attgtgcttg tgtctgtgct tgtgtctgtg cggcgatatg
    10261 gcgcgccgcg atccatccga aggtcatcgc cgggcccagc gtgatgccgc cgcccggata
    10321 atgcccgccc atgatgctgt tcatgtcgtt gcccgcggca taaagcccgg cgatcggcgc
    10381 gccttcccgg tccagcacgc gggcatgggc atcggttttc aggccggcga aggtgccaag
    10441 gctgccgggc acgatccgca ccgcgtaaaa cggcccggcc tcgatcggcg cgatgcaggg
    10501 gttgggccga tgatcgggat cgccctgggc gcggtgatag ggggttgtgc cccggccgaa
    10561 ggcggggtcg ctgccggatc gggcctgcgc gttccacgcc gcgatcgtgg cggccagccc
    10621 atcgggatcg atgccgcagg cctctgccag ttcgcgcagg ctgcggccct gcagcaggta
    10681 gccgttgcgc agccaccgcc cgatcgggac gggaaagggc ttgaccgcgc ccagcccata
    10741 acgccgcaga aagcgccggt cgcagatcag ccacgaggcc ggctcctgcc ctgccggcgt
    10801 agcggcgaaa agcgcgcgca tatagccgtg ataggacccg ccctcattga cgaaacgccg
    10861 gccggcgcgg gttacggcga tgaggcccgg tttgccgcgc tcgaccaggt ggggaaagcg
    10921 ggtgggctgg ccgtttttcc ccggcaccag cgaaaccggc gcccaggcga caggatcgac
    10981 ctgacctgta tcgaccagcg cccccagtgc ctcggccagg cgcaagccgt cgccggtgtt
    11041 gctggcgggg gcggcgctgt gatgggcaaa gccctcggcg acatggggaa ggagcaggct
    11101 ctggcgttgc cggtcatggg gaaagccgcc gcaggccagc accaccgcgc gcctggcccg
    11161 gatgaggccg acgccgccgg ccgtgcgcac ccgggccccg gtaacgcgcc ggccatcgct
    11221 ttccagcgcc tcgacgcatg tctgggtgag gagcgggacg ttgcggtcgg ccgccgatcg
    11281 taacaggcgg gcggtcaggg cattgccgtt caccagctgc atcgcgcggc catggcccag
    11341 cagatcgatc aggtggcggc ccagccgccg cagcacatgc agcatcgagg ccgccgaccg
    11401 aaaggcggtg agaaaggcgc gcagatcagg gcccggggca atggccatgc ccatcagcgt
    11461 ggtttcggcc agcggcgggg ccagttgcgc cagcagcggg cccagctgcc tgccgtcata
    11521 gggttctgcc acaagggagc gtccgccggt ggcggcctgg tcgatctcgc cgtgaaaatc
    11581 gggtgtggtc agccctggct ggaaatggac ctgtgtgtgc ctctcgaaaa aggccagcat
    11641 ttgggggccg gcatcgagaa aggcctggat cagggtgtcg tcataacgcg cgccaaggag
    11701 ggcgcgaagg tagcggaccg gggcttcgcg tggttcggaa aatccggctg cgcgcgcctc
    11761 gggcgaatgg gggatccaca accatccgcc ggaaatggct gtcgtgccac ccagcagcgg
    11821 cgctttctcg gcgacgatga catcgaggcc aaggtctgcc gccgtgaccg cggccgccat
    11881 cccggccgcg ccagagccga cgatcaggac atcgcaatca tgcagaacgg gcaggtcggg
    11941 catggcgtca gccttgcgcc gggaagggct tgaagcaggg catgggcagg ctgttcctct
    12001 ccgacctgtc tggggtgcga tcatgggatc tgatctgaat gacaggccat agtggaacat
    12061 cattccactg acaagccccg cttggccgga ggggcaaggc gcagatggca aaaggtttct
    12121 gcccgtcgat cggcatgccc cttgcgcaag gcgaggatcc ggcagaacca cgcaggcctg
    12181 ccggatcgtc agggtcagga atggctatgg gctggcgggc gtgagcggcg cttcaccggc
    12241 gccgccttcg cccggccggg cgcgcccgtt tttcagccct gttcggcggc gggggctgcg
    12301 cccagcacca gccgcaccag cgcgccgggg cgggggctgt ccacatgggc gagggcgggc
    12361 gcatgggcca gctcgaacag cagcgcgccc accatctggg catcatggcc cgcgcgccgg
    12421 gccagatcgg gcaggcgcgg cagcaggcac gcgcccagtt gctgccagca ggcgcgcagg
    12481 atcttctcct gctggcacag ggcctcggca ttgttctcgg cctggcgggc atattccatg
    12541 atggtcagcg cccaattgcc cgatccggcg cgcgcatcgg cccaatcggc cagcaggtcg
    12601 atcagcgtgg cggtgtcggg cgcggcgtgc agcgcttcca gcagcttggc catggattgc
    12661 gccccttcgg cctgcagcgc ggccaggaag atctcctcct tgcaggcgaa gtttgaatag
    12721 accgcgcctt tcgaatagcc ggccgcctcg gcgatgcggt cgatcgaggt ggcggcaaag
    12781 ccgtcgcggc gaaacagctc gcgcgcggca tcgatcagcc gctggcgggt ttgctcctgg
    12841 ctctggctac gcgtcaatcg cgacatgaag gccctccctt gggcgctcta tgttgccggg
    12901 cgccggcgcc gtcgagggtt tttgacgggc acgggttgaa atacccaaag gtatttatat
    12961 actgtatggt attctaatca atccgctcct ccctccgccg gctgtcgtgc gcagggagga
    13021 gcggcaccgg caaaggattc tgcggcatgg catggcaagg caaacgggtg gtggaactgg
    13081 cgccgagtgg ccaggtggat gccctggccc ccaccgtttc cctggccaac ggcgtggcca
    13141 tgcccgccat cggccttggc acctggccga tggatgatgc cgaggcggct ggcgctgtgg
    13201 cccgggctat cggccgtggc tatcgcctga tcgacacggc gcagaattac gggaacgagg
    13261 cgggcgtggg cgccggcgtg cgcgcctcgg gcgtggcgcg cgaggagatc ttcctcacca
    13321 ccaagttcaa ccgcgcatgg cacagcgtgg ccggcgtgcg cgaggcctgc gaggccagcc
    13381 tgaagcgcct gggcatggat tacatcgacc tctacctgat ccactggccc aaccccgatc
    13441 aggatcgcta tctcgatgca ttccacggcc tgcagcgggt ggccgaggcg gggctggtgc
    13501 gggcgatcgg cgtttccaat ttcaagccgg cgcatctggc gcggctgctg gatgccgggc
    13561 tggtgccgca ggtgaaccag atccagctcg atccctatca cccgcgcgcc gatatcgtgg
    13621 cgatccacca ggcccatggc atcgttaccg aaagctggag cccgatcggc cggggcaatg
    13681 ccctgctggc cgatccggcg atcctgcgca tcgcggccga tcatggccgc agcccggcgc
    13741 agatcgtgct gcgctggcag gtgcagcagg gcttcatccc cacccccaaa tccgctgatg
    13801 ccgggcggca ggcggccaat ctcgcgctgt tcgattttgc cctgagcgct gaggagatgg
    13861 ccgtgctggg cgggctgggc cgggcggatc ccgatatgat ggatgccgat gtgttcggcc
    13921 actagggctc agcggcgcaa ctgcgcgcgc gcggcgcggc ccacggcctc gctcagcgcg
    13981 cgcaggggcc ctgcgtgcag gcgcgccacc gtccagtaga gcggcacgtc gagcggggca
    14041 tcgggcacca gttccaccag ccggccggcc gcgatatgcg ggcgggccag cgcctctggc
    14101 tgcatcgccc agcccagccc gcgcaggccc agatcgagga aggcatgggt ggaaggcacc
    14161 caatgggtag tgcccgacag cggccctgtg ccgcgcctct gcgcccagct ggcctgcagc
    14221 ccgtcgcgcc ggtcgaagcg catgtggggg gcggtggcga gggcctcggg gccggggcct
    14281 tgcggaaaat ggcgcgccac gaaatccggg ctggcgcagg cgatatagcg cagcgtgccc
    14341 agcgggctgg tcttgcagcc gggcaccggg gcggggtcgg agctgaccac ggccatcacc
    14401 tcgccgctgc gcaggcgctc ggcggtgtgg gcctcatcgt ccagcgcgag ttcgatgaaa
    14461 cagccgctgg cgctggcaaa ggcggcgatg gcatcgggca accaggtggc caggctgtcg
    14521 gcattggcgg cgatcggcag ggtcacc
//
LOCUS       DASRYE010000293         9506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_64218,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000293 DASRYE010000000
VERSION     DASRYE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64218"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFF98_15100"
     CDS             <1..1161
                     /locus_tag="VFF98_15100"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU65011.1"
                     /translation="GASALGHWLLHKEPPADQPLPPGVVQLSASQMSGVTIVRAGAGE
                     AAERTEASGQIAADDTLSTPVYLPFSGQVTQVFVQAGDKVAAGQPLLAIRTGDVVDAR
                     NALIAAQAQRSAAQAQAKLAAANLARAEQVYKTAGGALKDYQQAQSDALATQSALRSA
                     ESALGAARDKLMVFGKSGAEVSRLEAARDPAGLRAETVFRAPVAGVIAQRSVAPGQYV
                     QAGAASPQLTITNPARVWMVAQLGESDAAHVHLGDRLDVLVPALGGRQLQAVVDNVAA
                     QVDPVSHRLAVRATLANADGALKPQMFASFIIHHPEKQAQAGVAVPASAVIHEGDAAR
                     VWVLVGPGRLQSRAVAVGGAADGEVRITAGLKAGEQVASTGAIFVNEAGLGQ"
     gene            1202..4354
                     /locus_tag="VFF98_15105"
     CDS             1202..4354
                     /locus_tag="VFF98_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZU65012.1"
                     /translation="MNDIIRLALRNRLLVVGFLIALLVAGGIAFSQLNIEAYPDPVPP
                     MVEIVTQTSGLSAEEMERNVTVPIEVQMAGIPHIKTIRGISLFGLSDVKIQFTYDYTF
                     DQAQQQVINRLAQMGSLAGGAQPQISPTSPIGEVYRYRVAGPAGYSPMDLKTIQDWVL
                     ERRFKAIPGVIDVTGWGGKLRSYDVVLDTARLNAHNATVAQVLQAISRSDTNVGGQTI
                     NFGAQAAIVRGVGLIHQVSQIGDSLVTVNNGVPVLVKDVAEVKIGNQPRLGIAGLGND
                     DDVIQGVVLMQRGAKSMPTIEAVKAEVDRVNASGILPPGVHLERIYDRSDLINHTTST
                     VIENLVAGVVLIFALQWLFLGNLRSAFIVAMTIPFALSVALLIIVLQGESANLLSVGA
                     LDFGLVVDATVIMVENIFRHMAERSSHVEGGHGHYSLATRLSAVMHAASEVSRGIFFA
                     AAIIIASFLPLFTLTGVEGHIFGPMAKTYAYAILGGLIATFTVAPVLSTMLLPDRLEE
                     VDTWLVGRMRRLYEPAARFALANRLLTLGGAGMLFVVALFGMSRLGVEFLPHLEEGNM
                     YIRAEMPASISLESGQPTVNAIRRAIARYPEAESVLSQQGRPDDGTDATGFFNAEFFV
                     PLKPAGQWPKGITKEQLTEQMNKDLTARFPGVAFNFSQYIEDNVEEAASGVKGANSVK
                     VFGPDLATLERIAGQIKDEMGRVRGITDLGVFQSLGQPTVRIDVDRARAARYGLQPDD
                     VNAVVQAAIGGQSPGDLYEKGTDRHFPIFVRLQPGQRDSVEAVKQIPIGAPSASGGTI
                     QVPLGEVAEVKLTTGASFIYREHQERYVPIKFSVRDRDLGGAVEEAQARIARNVVLPP
                     GYHLEWAGEMGNLTDAVARLEVVVPISLVLIFGLLMANFRSLRDSLLAFSVIPMAIIG
                     GVLALAISGTAFSISAAIGFVALFGIAVMDGILVVSTFNQALNEGSSRAEARHIILKN
                     CLRPVAMTCLVAAIGLLPAAVSTGIGSQVQKPLALVVVGGMTLAPLLILLVLPVLVEM
                     FSRHRRAEENRMRRPVEGEE"
     gene            4354..5802
                     /locus_tag="VFF98_15110"
     CDS             4354..5802
                     /locus_tag="VFF98_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZU65013.1"
                     /translation="MRKFGALALLLPLAGCITPGPRAETTLPLAPAAAGNAAIAPIRP
                     EHGPAQVFVEGGHVPARWWQGFASPALDALVEQALAGNNDIAAADAALRQARSLAGAT
                     AAGALPQVTAGYSAQRAKASDIVSPPLADPNDTLYTLHTAQLSVTYPLDVFGGGRAKI
                     RSARAAAEAQDFRTRAARTTVVANLVLAVIQHAQVQEQLAAARQAMAAGRDLLALLQR
                     RRELGAAGDADVAAQEATLAALEASVPGLERTALHQQALIAAFIGVAPGTPLPPLPTL
                     ADLHLPAELPVAVPSQLVAERPDVRAAAAQMKGAAADVGAAIAARLPAFQISGNVGGS
                     ALKFGEMFADGNLFWQVLGEVAQPIFDGGQLLKQKHAADAAFVAAKAQYRASALQAFV
                     DVSDALEGLSTDAAALDAAARGDGAAARSLAYTLRQNQLGGVGVFDLRNAEVARAQAA
                     QTYAQAKAARLADTVALYQALGAGVVAGEPRK"
     gene            6001..6588
                     /locus_tag="VFF98_15115"
     CDS             6001..6588
                     /locus_tag="VFF98_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZU65014.1"
                     /translation="MRGGFFGGGGPFGGGPFGGGPFGDEFGGGRGGRGGGGGGRRAKR
                     FDSEELRLLVLGELGEEPRHGYQLIRALAEKSADAYQPSPGVLYPLLTMLAEMGLVAE
                     AEGQEGSTRRRYELTEAGRAELAANAERLAQLRARLAALAEQAGRIDGAPVRRAVHNL
                     RAAIMERLGRAGTAEETAFAVAAIIDEAAQKIERL"
     gene            6609..6896
                     /locus_tag="VFF98_15120"
     CDS             6609..6896
                     /locus_tag="VFF98_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HZU65015.1"
                     /translation="MPLSQAIVPTASASRYMQQLAKHWSHKFPVSFDATRASFPLPLG
                     PVTMLADAAALTVTCEVAEGGDIERLQQVVAEHLNRFAFREGELVFDWQPA"
     gene            6959..7402
                     /locus_tag="VFF98_15125"
     CDS             6959..7402
                     /locus_tag="VFF98_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HZU65016.1"
                     /translation="MIAHTIQLALAPVFVLVAIGNIMNILSTRLGRIVDRARQLQLMY
                     HETQGLRHDLVVVEMRAVDQRILLNSRAIHVLVMSGLSIGITVSILFVEEVVSVPLER
                     VAACFFLVAVALLMWGLTLFLRETRLAADLLRIPEDYLERHRELE"
     gene            complement(7454..9238)
                     /locus_tag="VFF98_15130"
     CDS             complement(7454..9238)
                     /locus_tag="VFF98_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZU65017.1"
                     /translation="MTETPVLDRTDAPQMPIPTPNACLLPRRLADFSSFTEALDYAAK
                     GVRGLNFHDARGNLALAYPYARLRADALAMAYRLIARGVRPGDRIALVAETGTEFAAL
                     FCGAIYAGAWPVPLPLPTSFGGKDNYIDQLAVQLGSSDPALFFYPPEIATMGAGAAAA
                     HNAKGGGCLALSWDVLAAEEATPAVLPQLAGDDICYLQYSSGSTRFPHGVAVTHASLL
                     NNLAGQGEGLQMCRHPEDRVVSWLPWYHDMGLVGCFLSPIANQISVDYMKTEDFARRP
                     LAWLDMISRNPGESISYSPTFGYDICARRISSQTNVAERFDLSRWRVAGNGADMIRPD
                     VMQGFVNVFAAAGFRAAAFLPSYGLAEATLSVTLMPVGEGIRVELVEEERLSGTPRDL
                     SRPARYRAIVNCGKPVKDMALEIRDGAGKLLADHQIGKVWCKGPSIMHSYFRDPESTA
                     DCLVDGWLDTGDMGYTVDGYLFIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFHQGDI
                     AAFSLQTETGEETAAVLVHCRVADPAERLRLRDAVADKVRSVLGMSAVVELVPPRTLP
                     RTSSGKLSRAKAKRLYLSGEIAPLQLAA"
BASE COUNT         1460 a         3083 c         3385 g         1578 t
ORIGIN      
        1 ggggcctcgg cgctgggcca ctggctgctg cacaaggagc cgccggccga tcagccgctg
       61 ccgccgggcg tggtgcagct ttccgccagc cagatgagcg gcgtgacgat cgtgcgcgca
      121 ggcgctggcg aggcggccga gcgcaccgag gccagcggcc agatcgccgc cgacgacacg
      181 ctctccaccc ctgtctacct gccgttcagc ggccaggtca cccaggtttt cgtgcaggcg
      241 ggcgacaagg tggccgccgg ccagccgctg ctcgctatcc gcaccggcga tgtggtggat
      301 gcgcgcaacg cgctgatcgc cgcccaggcc cagcgcagcg ccgcccaggc ccaggcgaag
      361 ctggccgcag ccaacctcgc ccgcgccgag caggtgtata aaaccgccgg cggcgcgctg
      421 aaggattacc agcaggcgca aagcgatgcg ctggccaccc aatcggccct gcgcagcgcc
      481 gaaagcgcgc tgggcgccgc gcgcgacaag ctgatggtgt ttggcaagag cggggccgag
      541 gtgagccggc tggaggccgc gcgcgatccg gcgggcctgc gtgccgaaac ggtgttccgc
      601 gcgccggtgg ccggggttat cgcccagcgc agcgtggcgc cggggcaata tgtgcaggcg
      661 ggcgcggcca gcccgcagct caccatcacc aaccctgcgc gggtgtggat ggtggcgcag
      721 ctgggcgaat cggatgccgc ccatgtccat ctgggcgatc ggctggatgt gctggtgccg
      781 gcgctgggcg ggcgccagtt gcaggcggtg gtcgataatg tggccgccca ggtcgatccg
      841 gtgtcgcacc ggctggccgt gcgcgccacg ctggccaatg ccgatggggc gctgaagccg
      901 cagatgtttg ccagcttcat catccatcac ccggaaaagc aggcgcaggc cggcgttgcc
      961 gtgcccgcca gcgcggtgat ccacgagggc gatgcggccc gcgtgtgggt gctggtgggg
     1021 cccgggcggc tgcaatcgcg cgcggtggcc gtgggcggcg cggccgatgg cgaggtgcgc
     1081 atcaccgccg gtctcaaagc cggcgagcag gtggccagca ccggcgccat tttcgtcaac
     1141 gaagccgggc tgggccagtg agcctggcgg cgcgggccga tagccgggcc ggggggcagg
     1201 catgaacgac attatccgcc ttgccctgcg caaccgcctg ctggtggtgg gctttctcat
     1261 tgcgctgctg gtggctggcg gcatcgcctt ttcccagctg aacatcgagg cctatcccga
     1321 tccggtgccg ccgatggtcg agatcgtcac gcagacctcg ggcctttcgg ccgaggagat
     1381 ggaacgcaac gtcaccgtgc cgatcgaggt gcagatggcg ggcattcccc acatcaagac
     1441 gatccgcggc atttcgctgt tcggcctgtc ggatgtgaag atccagttta cctacgacta
     1501 caccttcgat caggcccagc agcaggtgat caaccggctg gcgcagatgg gcagcctggc
     1561 cggcggcgcg cagccgcaga tttcgcccac cagcccgatc ggcgaagtct atcgctaccg
     1621 tgtggcaggg cccgccggct attcgccgat ggatctcaag accatccagg actgggtgct
     1681 ggaacgccgc ttcaaggcca ttcccggcgt gatcgacgtg accggctggg gcggcaagct
     1741 gcgcagctat gacgtggtgc tcgataccgc gcggctgaat gcccataacg ccaccgtggc
     1801 gcaggtgctg caggcgattt cccgcagcga caccaatgtc ggcgggcaga ccatcaactt
     1861 cggcgcgcag gccgcgatcg tgcggggcgt ggggctgatc caccaggtca gccagatcgg
     1921 cgattcgctg gtaacggtga acaatggcgt gccggtgctg gtgaaggatg tggccgaggt
     1981 gaagatcggc aaccagccgc gcctgggcat cgccggcctt ggcaacgacg acgacgtgat
     2041 ccagggcgtg gtgctgatgc agcgcggcgc caagagcatg ccgacgatcg aggcggtgaa
     2101 ggccgaggtg gaccgcgtca atgccagcgg catcctgccg ccgggcgtgc atctggaacg
     2161 catctacgac cgctccgacc tcatcaacca caccaccagc acggtgatcg agaatctggt
     2221 ggctggcgtg gtgctgatct ttgccctgca atggctgttc ctgggcaatc tgcgcagcgc
     2281 cttcatcgtg gcgatgacga tcccctttgc cctttcggtg gcgctgctga tcatcgtgct
     2341 gcagggcgaa tcggccaatc tgctttcggt gggcgcgctc gatttcggcc tggtggtgga
     2401 tgcgacggtt atcatggtgg agaacatctt ccgccacatg gccgaaagat cctcgcatgt
     2461 ggaaggcggc catggccatt attcgctggc cacgcgcctg tcggcggtga tgcatgcggc
     2521 cagcgaagtg agccggggca tcttctttgc cgccgccatc atcatcgcca gcttcctgcc
     2581 gctgttcacg ctgacaggtg tggaaggcca catcttcggc cccatggcca agacctatgc
     2641 ctatgccatc ctcggcgggc tgatcgccac tttcaccgtg gcgccggtgc tttccaccat
     2701 gctgctgccc gaccggctgg aagaggtgga tacctggctg gtgggccgca tgcgccgcct
     2761 gtatgagccg gccgcgcgct ttgccctggc caaccggctg ctcacgctgg gcggggcggg
     2821 gatgctgttt gtggtggccc tgttcggcat gagccggctg ggggtggaat tcctgccgca
     2881 tctggaagag ggcaacatgt atatccgggc cgaaatgccc gcttccatca gcctggaatc
     2941 cggccagccc acggtgaatg ccatccgccg cgccatcgcc cgctatccgg aggccgaaag
     3001 cgtgctctcg cagcagggcc ggccggatga tggcaccgat gccaccggct tcttcaacgc
     3061 cgaattcttc gtgccgctga agccggccgg ccagtggccc aagggcatca ccaaggaaca
     3121 gctgaccgag cagatgaaca aggacctcac cgcccgcttc ccgggggtgg cgttcaactt
     3181 cagccagtat atcgaggaca atgtcgagga agcggcgagc ggcgtgaagg gggccaattc
     3241 ggtcaaggtg ttcggccccg atcttgccac gctggagcgc attgccggcc agatcaagga
     3301 cgagatgggc cgggtgcgcg gtatcaccga tctcggcgtc ttccagtcgc tgggtcagcc
     3361 caccgtgcgc atcgatgtcg atcgcgcccg ggctgcccgc tatggcctgc agcccgatga
     3421 tgtgaatgcg gtggtgcagg ccgcgatcgg cggccagtcg cccggcgatc tctacgagaa
     3481 gggcaccgat cgccacttcc cgatcttcgt gcgcctgcaa ccgggccagc gcgacagtgt
     3541 ggaagcggtg aagcagatcc ccatcggcgc gccttcggcc agcggcggca cgattcaggt
     3601 gccgctgggc gaggtggccg aggtgaagct gaccaccggc gccagcttca tctaccgcga
     3661 acatcaggaa cgctatgtgc cgatcaagtt ttcggtgcgt gatcgggatc tgggcggcgc
     3721 ggtggaagag gcgcaggcgc gcatcgcccg caatgtggtg ctgccgcccg gctatcatct
     3781 ggaatgggcg ggcgagatgg gcaatctcac cgatgcggtg gcgcggctgg aagtggtggt
     3841 gccgatcagc ttggtgctga tcttcggcct gctgatggcc aatttccgct ccctgcgaga
     3901 cagtctgctg gccttcagcg tgatccccat ggcgatcatc ggcggcgtgc tggcgctggc
     3961 gatcagcggc acggccttct ccatctcggc ggcgatcggc tttgtggcgc tgttcggcat
     4021 tgcggtgatg gatggcattc tggtcgtctc cacttttaac caggcgctca acgaaggctc
     4081 cagccgggcc gaggcgcgcc atatcatcct caagaactgc ctgcgcccgg tggcgatgac
     4141 ctgccttgtg gcggcgatcg gcctgctgcc ggcagcggtt tccaccggca tcggcagcca
     4201 ggtgcaaaag ccgctggcgc tggtggtggt gggcggcatg acgctggccc cgctgctgat
     4261 cctgctggtg ctgccggtgc tggtggagat gttctcgcgc catcgccggg ccgaggaaaa
     4321 ccgcatgcgc cgcccggtgg agggagagga atgatgcgta aattcggcgc tcttgccctg
     4381 ctgttgccgc tggccggctg catcacgccc ggccccaggg ctgaaacgac cctgccgctg
     4441 gccccggccg ctgcgggcaa tgccgccatt gcccccatcc ggcccgaaca tggcccggcg
     4501 caggttttcg tggaaggcgg ccatgtgccg gcgcgctggt ggcagggctt tgccagcccc
     4561 gcgctcgatg cgctggtgga acaggcgctg gctggcaaca acgacatcgc cgctgccgat
     4621 gccgccctgc gccaggcccg gtcgctggcc ggggcgacgg cggccggcgc cctgccgcag
     4681 gtcacggcgg gctattccgc ccagcgcgcc aaggcgtcgg acatcgtctc gccgccgctg
     4741 gccgatccca acgacacgct ctacacgctg cacacggcgc aattgtccgt cacctatccg
     4801 ctcgatgtgt tcggcggcgg gcgggcgaag atccgctcgg cccgcgccgc ggccgaggcg
     4861 caggatttcc gcacgcgcgc ggcgcgcacc acggtggtgg ccaatctggt gctggcggtg
     4921 atccagcacg cgcaggtgca ggagcagctg gccgccgccc gccaggcgat ggccgccggc
     4981 cgtgatctgc tggccctgct gcaacgccgg cgcgagctgg gcgcggcggg cgatgccgat
     5041 gtcgccgcgc aggaagcgac gctcgccgcg ctggaagcca gcgtgccggg gctggagcgc
     5101 acagcgctgc accagcaggc gttgatcgcc gccttcatcg gcgtggcgcc gggcacgccg
     5161 ttgccgcctc tgccaacgct tgccgatctg cacctgccgg ccgagctgcc ggtggccgtg
     5221 ccctcgcagc tggtggcaga gcggcccgat gtgcgcgccg ctgccgccca gatgaagggc
     5281 gcggcggccg atgtgggcgc ggcgattgcc gcgcgcctgc ccgccttcca gatcagcggc
     5341 aatgtgggcg gcagcgcgct caaatttggc gagatgttcg ccgatggtaa cctcttctgg
     5401 caggtgctgg gcgaggtggc gcagccgatc ttcgatggcg gccagctgtt gaagcagaag
     5461 cacgccgccg atgccgcttt cgtggcggcc aaggcgcaat atcgcgccag cgcgctgcag
     5521 gcctttgtcg atgtgtcgga tgcgctggaa gggctgtcca ccgatgcggc ggcgctggat
     5581 gcggcggcgc gcggcgatgg cgcggcggcg cgcagcctgg cctatacctt gcggcagaac
     5641 cagctgggcg gggtgggcgt gtttgatctg cgcaacgccg aggtggcccg cgcgcaggcc
     5701 gcgcaaacct atgcccaggc caaggcggcg cggttggccg atacggtggc gctgtatcag
     5761 gcgctggggg ctggcgtggt ggcgggggaa ccgcgcaaat gagtttctga aaatcactca
     5821 agatatatct tgaaaacatt ttgaagaatg ttatctctcc tcgcatccaa gagatgcaag
     5881 gagtttcgaa tcatgtttgg atatgacaag ggccgcaggg gcggcccgga tcgtggtggc
     5941 gatcgcggcg gcgaaggccg gggatgcggc ggtggccgtg ggcgccgggg cctgttggcc
     6001 atgcgcggcg gctttttcgg cggtggcggc ccgtttggcg gtggcccctt cggcggcggg
     6061 ccgtttggcg atgagtttgg cggcggccgg ggcgggcgcg gcggcggtgg cggtggccgc
     6121 cgggccaagc ggttcgacag cgaggagctg cggctgctgg tgctgggcga gctgggcgag
     6181 gaaccgcgcc atggctatca gctgatccgc gcgctggccg aaaagagcgc cgatgcctat
     6241 cagcccagcc ccggcgtgct ttatccgctg ctcaccatgc tggccgagat gggcctggtg
     6301 gccgaggcgg aggggcagga aggctccacc cgccgccgct acgagctgac cgaggccggc
     6361 cgcgccgaac tggccgccaa tgcagagcgc ctggcccagc tgcgggcacg gctggccgcg
     6421 ctggccgaac aggctggccg cattgatggt gcgccggtgc gccgggcggt gcataatctg
     6481 cgcgcggcga tcatggagcg gctgggccgc gccggcaccg ccgaggaaac cgcctttgcc
     6541 gtggccgcca tcatcgatga ggccgcccag aagatcgagc gcctgtgagg gcctcgcagg
     6601 agaagaccat gcccctttcg caagccatcg tgcccaccgc cagcgccagc cgttatatgc
     6661 agcagctggc caagcactgg agccacaagt tcccggtcag cttcgatgcc acccgcgcca
     6721 gcttcccgct gccgctgggc ccggtgacga tgctggccga tgcggcggcc ctgacggtga
     6781 cttgcgaagt ggccgagggc ggcgatatcg agcggctgca gcaggtggtg gccgaacacc
     6841 tcaaccgctt cgccttccgc gagggcgagc tggtgttcga ctggcagccg gcctgatacc
     6901 ggccctggtt gcgaaggccc gctggcatgc cggcgcgcct tcgcctagaa gccggggcat
     6961 gatcgctcat accatccagc tggcgctggc cccggtcttc gtgctggtgg ccattggcaa
     7021 tatcatgaac atcctgtcca cccggctggg gcgcatcgtc gatcgcgccc gccagctgca
     7081 gctgatgtat cacgagacgc aagggctgcg gcacgatctg gtggtggtgg aaatgcgcgc
     7141 ggtcgatcag cgcatcctgc tcaattcgcg ggccatccat gtgctggtga tgtcgggcct
     7201 gtcgatcggc atcacggtgt cgatcctctt cgtcgaggaa gtcgtcagcg tgccgctgga
     7261 gcgggtggcg gcgtgtttct tcctggtggc ggtggcgctg ctgatgtggg gcctcacgct
     7321 gttcctgcgc gagacgcggc tggccgccga tctgctgcgc atcccggaag actatctgga
     7381 gcgccaccgc gagctggaat gaaaaaaggg gcgggggttt tcaccctcgc cccctttttt
     7441 cgctgcggcg ttttcaggcc gccagctgca gcggggcgat ctcgcccgag agatacagcc
     7501 gcttggcctt ggcgcggctg agcttgcccg agctggtgcg cggcagggtg cgcggcggca
     7561 ccagttccac caccgcgctc atccccagca ccgagcgcac cttgtcggcc accgcatcgc
     7621 gcaggcgtag ccgctcggcc ggatcggcca cgcggcagtg caccagcacg gccgcggttt
     7681 cctcgccggt ttccgtctgc aggctgaagg cggcgatatc gccctggtgg aagccgggca
     7741 gctgttccac cgcccattcg atatcctgcg gccagtggtt cttgccgttg atgatgatca
     7801 tgtccttcgc ccggccgacg atgaacagat agccatccac ggtatagccc atgtcgccgg
     7861 tgtcgagcca gccatcgacc aggcagtcgg cagtcgattc cgggtcgcgg aagtaggaat
     7921 gcatgatgct ggggcccttg caccacacct tgccgatctg atggtcggcc agcagcttgc
     7981 cggcgccatc gcggatttcc agcgccatgt ccttcaccgg cttgccgcaa ttgacaatgg
     8041 cccgatagcg cgccgggcgc gacagatcgc gcggcgtgcc cgacaggcgc tcttcctcca
     8101 ccagctccac ccggatgcct tcgcccaccg gcatcagcgt caccgacagg gtcgcctcgg
     8161 ccaggccata ggagggcagg aaggccgcgg ccctgaagcc ggccgcggca aacacgttga
     8221 caaagccctg catcacatcg gggcggatca tgtcggcgcc attgcccgcc acgcgccagc
     8281 gcgacaggtc gaagcgttcg gccacgttgg tctggctgga gatgcggcgg gcgcagatgt
     8341 catagccgaa agtgggcgaa tagctgatcg attcgcccgg gttgcggctg atcatgtcga
     8401 gccaggcgag cgggcgccgg gcgaaatcct cggtcttcat gtagtcgacc gagatctggt
     8461 tggcgatcgg cgagaggaag cagccgacca gccccatgtc gtgataccag ggcagccagc
     8521 tcaccacccg gtcttccggg tgccggcaca tttgcagccc ttcgccctgg ccggccagat
     8581 tgttcagcag cgaggcatgg gtaaccgcca cgccatgcgg aaagcgcgtg gagcccgagg
     8641 aatattgcag atagcagata tcatcgccgg ccagctgcgg cagcacggcc ggcgttgctt
     8701 cctcggccgc cagcacatcc cacgacaggg ccaggcagcc gccgcccttg gcgttgtgcg
     8761 cggcagccgc gcccgcgccc atggtggcga tttcgggcgg atagaagaac agcgccggat
     8821 cagagctgcc cagctgcacg gccagctggt cgatgtaatt gtccttgccg ccaaagctgg
     8881 tgggcagcgg cagcggcacc ggccaagcgc cggcatagat ggcgccgcag aagagggcgg
     8941 caaattcggt gccggtttca gcgaccaggg caatccggtc gcccgggcgc acgccgcggg
     9001 cgatcagccg gtaggccatc gccagcgcat cggcgcgcag ccgggcatag ggataggcca
     9061 gcgccagatt gccgcgcgca tcgtggaagt tgaggccccg cacacccttg gcggcataat
     9121 ccagcgcttc ggtaaaggag gaaaaatcgg ccaggcggcg cggcagcagg caggcgttgg
     9181 gcgtggggat cggcatttgc ggcgcgtcgg tgcgatccag cacgggggtc tcggtcatcg
     9241 ggttgctcgg tccatcctgg cgggctggcg gggccagcgt gctgttgttt tggcgcttct
     9301 tatcgggcac acacccggat gagccgggca tgaacgcgaa cccctgccgc aagggcaggc
     9361 tgtcgattgt tgccctttgc gcggtgagtg tggcacgaat aaggccgatg gctggtttta
     9421 accataagga gcggcgcccc cccgtggcgc tggaggcaga gcggctggag cagctggcgc
     9481 tggcctatgt ggcgcgcttt gccacc
//
LOCUS       DASRYE010000294         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_105167,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000294 DASRYE010000000
VERSION     DASRYE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105167"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2446
                     /locus_tag="VFF98_15135"
     CDS             2..2446
                     /locus_tag="VFF98_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZU65018.1"
                     /translation="MRLLLACLFLGVGALAAIGSLTSAISAGLAAKGRLILGGDVQIT
                     VWQRAFTPAERAALAAHGRLSDGLRLQAMASAGDTAAPVQLKAVDSAWPLVGRLQLAD
                     GRQVGAPAPGSAWIAAGAAARLGLKPGDSFTIAGHPLRVAGLIGTEPDGLGEGFALGP
                     TVIVPLDFPGRAGLIQPGAMFRSRLRLACAQSCDAAAMADDLAHRFPDAGLDIRTRDK
                     AAPGTETFLARMGDFLVLVGLSALLIAGIGIGGGTASYLEARRGVIATLKVLGATSGD
                     IARIYLLQITAAALVGALAGLVAGVIALPLITRALAGVLPVADGFVLAPWPLARAGLW
                     GLLVALVFAAPPLAAARSFPAMALLRARVAPVSGRGGWQWVAGGLAGIVGLAFLGGGA
                     PRVTALFLGGAAAILLLLAGLGLAIRRAARALPRPRGTIARMALANLHRPGAQTARLV
                     TALGFGLTAFVVLAVVQTSLDANIRARVPQKAPDYFVVDLQPADEATLRATVAGLAPA
                     ARVRAVPTLRGAILAYGPPGRQARVADMKDIPEDAWQLKGERGLTFASQIPEGNVLTD
                     GAWWPADYAGEPLVSVDEKLARSIGLHVGDSLTVGLLGVERTARIASLRRIDWDSFGF
                     NYVLVFSPNALAGAPFKLAATISLPGGQANGPLLRALTRAFPTASVIEVGPLLRDARG
                     LLGQMSTAILAAASVAVLAGIAVLLGALAAARAARAYDNTILRVLGAGRRQLLALLLA
                     EYGALAALLAAVALGLGTGLGWLIVVKLFGFDFLPGWGRILAVLGAGLALVLGFALGG
                     SLPLLRTRPAQALREL"
BASE COUNT          292 a          957 c         1064 g          450 t
ORIGIN      
        1 cttgcggctg ctgctggcct gcctgttcct gggggtgggc gcgctggcgg cgatcggctc
       61 gctcaccagc gcgatctcgg cggggctggc ggccaagggc cggctgatcc tgggcggtga
      121 tgtgcagatc accgtgtggc agcgcgcctt cacgccggcc gaaagggcag cactggccgc
      181 ccatggccgc ctgtctgacg gactgcgcct gcaggcaatg gccagcgccg gcgacactgc
      241 cgcgcccgtg cagctgaaag cggtggacag cgcctggccg ctggtgggcc gccttcagct
      301 ggccgatggg cgccaggtgg gcgcgccggc acccggcagc gcgtggatcg cggcgggcgc
      361 ggcggcgcgg ctggggctca aacccggcga cagtttcacc atcgccggcc accccttgcg
      421 cgtggccggc ctgatcggca ccgagcccga cgggctgggc gaaggctttg cgctggggcc
      481 cacggtgatc gtgccgctgg attttccggg ccgcgcgggg ctgatccagc cgggcgccat
      541 gttccgcagc cggctgcggc tggcctgcgc gcaaagctgc gatgcggcgg ccatggccga
      601 tgatctggcc catcgtttcc ccgatgccgg gctggatatc cgcacgcgcg acaaggccgc
      661 ccccggcacc gagaccttcc tcgcacggat gggcgatttc ctcgtgctgg tgggcctttc
      721 ggcgctgctg attgccggca tcggcattgg cggcggcacg gcttcctatc tggaggcgcg
      781 gcggggggtg atcgccacgc tgaaggtgct gggcgccacc agcggcgata tcgcccggat
      841 ctatctgctg cagatcaccg ctgccgcgct ggtgggtgcg ctggccgggc tggtggcggg
      901 cgttatcgcc ttgccgctga tcacccgtgc gctggccggc gtgctgccgg tggcagacgg
      961 ctttgtgctg gcgccctggc cgctggcgcg ggcgggcctg tgggggctgc tggtggcgct
     1021 ggtgtttgcc gccccgccgc tggccgccgc gcgcagcttt ccggcgatgg cgctgctgcg
     1081 cgcgcgggtg gcgccggtgt ctggcagggg gggctggcag tgggtggcag gcgggctggc
     1141 ggggattgtc ggcctggcgt tcctcggtgg cggcgcgccg cgggtcacgg cgctgttcct
     1201 gggcggggcg gcggcgatcc tgctgctgct ggcggggctg gggctggcga tccgccgcgc
     1261 cgcccgcgcc ctccctcgcc cgcgcggcac gatcgcgcgg atggcgctgg ccaacctcca
     1321 ccgccccggc gcgcagacgg cgcggctggt aaccgcgctg ggctttggcc tcaccgcctt
     1381 tgtggtgctg gccgtggtgc agaccagcct tgatgccaat atccgcgccc gtgtgccgca
     1441 aaaggcgccc gactatttcg tggtggacct gcaaccggcc gacgaggcga ccctgcgcgc
     1501 caccgtggcc ggcctggccc ctgccgcaag ggtgcgcgcg gtgcccaccc tgcgcggggc
     1561 gatcctggcc tatggcccgc ccggccggca ggcgcgcgtg gccgacatga aggacattcc
     1621 cgaggatgcc tggcagctga agggcgagcg cgggctgacc ttcgccagcc agatccccga
     1681 gggcaatgtg ctgaccgatg gcgcctggtg gccggccgat tatgccggcg agccgctggt
     1741 ctcggtggac gagaagctgg cccgcagcat tggcctgcat gtgggcgaca gcctcaccgt
     1801 gggcctgctg ggggtggagc gcaccgcccg catcgcctcg ctgcggcgga tcgactggga
     1861 cagtttcggc ttcaactatg tgctggtgtt ctcgcccaat gcgctggcgg gcgcgccgtt
     1921 caagctggcg gccaccatca gcctgcccgg cggccaggcc aatggcccgc tgctgcgcgc
     1981 gctcacccgc gcctttccca ccgcttctgt catcgaggtg gggccgctgc tgcgcgatgc
     2041 gcgcggcctg ctggggcaga tgagcacggc catcctggcc gccgccagcg tggccgtgct
     2101 ggccggcatt gccgtgctgc tgggagcgct ggccgccgcc cgcgcggccc gcgcctacga
     2161 caacaccatc ctgcgcgtgc tgggcgccgg gcgcagacaa ttgctggcgc tgctgctggc
     2221 ggaatatggc gcgctggcgg ccctgctggc ggcagtggcg ctggggctgg gcacggggct
     2281 gggctggctg atcgtggtca agctgttcgg cttcgatttc ctgccgggct gggggcggat
     2341 cctggccgtg ctgggggcgg ggctggcgct ggtgctgggc tttgcgctgg gcggatcgct
     2401 gccgctgctg cgcacccgcc cggcccaggc cctgcgcgag ctataacccc catcccccgc
     2461 catctcgcaa tcccaccttg tgaaaacccc gtagatcgct aggctggaga gcggacagtg
     2521 cgtgcaagcc atccttgcac atgggggcac tttcggggga atacgggaaa aatgggcatg
     2581 gcttgcgcgc ggcgcgcctt ggctggcaga tcaaaccgac cgggcggcag gcctcctgcc
     2641 gccgggcata gcgggctggc cggcatggcc ttccttttgc gccgcgcacg ctaatgcccg
     2701 gcccgattgc ggccatcggc cctgccgggc tggccggctt gctggcgctg accccgctgg
     2761 ccg
//
LOCUS       DASRYE010000295         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_169320,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000295 DASRYE010000000
VERSION     DASRYE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169320"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFF98_15140"
     CDS             complement(<1..1263)
                     /locus_tag="VFF98_15140"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU65019.1"
                     /translation="MTEEIPQNPPAPVASYRVEQAVAVLTIDSPPVNALGHRVRLAIA
                     EGVERALADGGVQALVLVCAGRTFFAGADVSELGKPILPPLLGDIIALFEASPKPVVA
                     AMHGTALGGGFELALAAHFRVAVAAAAVGLPEVALGLLPGAGGTQRVPRLVGVRAAVE
                     MIGLGRQVKAPEALELGLIDAIVADDALVEGAVAFARAQVAAGARLRRLRDLPTDMGG
                     QEAQAVFAAFRAAHPDLFIGFKAAEGVLQAIEAAATLPFDAGLARERALSQALTASPE
                     SAAQRHLFFAARAAARLPGSEKLRAAPAEAVAVIGPWAGAAGLRAAGVAVADAVLPGR
                     PALLGTGARPEAAEGASCLIHTEGAATLPADAIGLMLHAGVAEIVVGQDASPATAVTA
                     MALAQQAGWPAIYVRPAPGLVVARLAGRL"
     gene            1392..2381
                     /locus_tag="VFF98_15145"
     CDS             1392..2381
                     /locus_tag="VFF98_15145"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZU65020.1"
                     /translation="MPSPIAADLRAQLTLPAICAPMMILSGPELVTAACKAGLMGALP
                     RHNARTEAEFASWLARIRADLDAHAAACPGARIGPVAVNFVLSRPVAELLPEIDQCIA
                     HGIRHFITALGNPAEMAHAVHQRGGLIFHDVTNLRFARKAAEAGVDGLICIGGGGGGH
                     SGTLNPFAFIPKVRAFFDGVIVYAGCVTDGAAIRAAEVLGADLAYVGTRFIATCEARA
                     PEAYKQMLIAGGAEDLRYSPGITGVNANWLAASLERCGVDLEALGAFSRDYAHLPPDI
                     RPWRDVWSAGQGVELIEDCPDAASLVARLRREYVAACAVPDLAASARQAEPAL"
     gene            2378..>2629
                     /locus_tag="VFF98_15150"
     CDS             2378..>2629
                     /locus_tag="VFF98_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU65021.1"
                     /translation="MTPADLLAGADSPLVIADRGGLRFVLLNRPAARNALTRAMRADF
                     ARLLAEAEADDSVTALVLTGAGGCFSAGVDLKDRIPGAAP"
BASE COUNT          387 a         1035 c          856 g          351 t
ORIGIN      
        1 caagcgcccc gccagccggg ccaccaccag ccccggggcc gggcgcacat agatcgccgg
       61 ccagccggcc tgctgggcca gcgccattgc cgtcaccgcc gttgccgggc tggcgtcctg
      121 ccccaccacg atttccgcca cgcctgcatg cagcatcagg ccaatcgcgt cggcgggcag
      181 ggtggccgcc ccctcggtgt gaatgaggca gctggcccct tccgccgcct ctggccgggc
      241 gccggtgccc aacagcgccg gccgccccgg cagcaccgca tccgccaccg ccacgccagc
      301 cgcacgcagc cctgccgcgc ccgcccatgg cccgatcacc gctaccgcct ctgccggcgc
      361 ggcgcgcagc ttctcgctgc ccggcagcct ggccgcggcc cgcgcggcga aaaagagatg
      421 gcgctgggcc gccgattccg ggctggccgt caacgcctgc gacagcgcgc gttcgcgggc
      481 cagcccggcg tcgaacggca gggtggctgc cgcctcgatc gcctgcagca ccccttcggc
      541 cgccttgaag ccaatgaaca gatcgggatg ggcggcgcga aaagcggcaa acaccgcctg
      601 cgcctcctgc ccccccatat ccgtgggcag atcacgcagg cgccgcaagc gcgcgcccgc
      661 cgccacctgc gcccgggcaa aggccaccgc cccttccacc agcgcgtcat cggccacaat
      721 cgcgtcgatc agccccagtt ccagcgcctc gggcgccttc acctgccggc caaggccgat
      781 catctccacc gccgcgcgca cgccaaccag gcgaggcacc cgctgcgtgc cgcccgcccc
      841 cggcagcagg cccagcgcca cttcgggcag gcccaccgcc gccgcggcca ccgccacgcg
      901 gaaatgcgcc gccagcgcca gttcaaagcc gccccccagc gccgtgccat gcatggccgc
      961 caccaccggc ttgggcgagg cttcgaacag ggcgatgata tcgcccagca gcggcggcag
     1021 gatcggcttg cccagctcgc tcacatcggc gccggcaaag aaggtgcgcc cggcgcaaac
     1081 cagcaccagg gcctgcaccc cgccatcggc cagcgcgcgt tccacgccct cggcaatggc
     1141 caggcgcacg cggtggccca gcgcgttcac cggcggcgaa tcgatggtca gcaccgccac
     1201 cgcctgttcg acacgatagg aagcgacggg cgccggcggg ttttgcggaa tttcctcggt
     1261 catggcgcag caattacttg acgcggggcg ggcgaacaag tcaaaaagtc cgatatacga
     1321 tcataaggtt gattatcgca cattggcgag gccataaccc gcccggtgcc cgagaggacg
     1381 aagacagcgc catgcccagc ccgattgccg ccgacctgcg cgcccagctc accctgccgg
     1441 cgatctgcgc gccgatgatg atcctgtccg gcccggagct ggtgacggca gcctgcaagg
     1501 ccgggctgat gggcgccctg ccgcgccaca atgcccgcac cgaagcagaa ttcgccagct
     1561 ggctcgcccg catccgcgcc gatcttgatg cccatgccgc cgcctgcccg ggcgcccgga
     1621 tcggcccggt ggcggtgaat ttcgtgctca gccggccggt ggccgaactg ctgcccgaga
     1681 tcgaccagtg catcgcccac ggcatccgcc acttcatcac cgcgcttggc aatccggcgg
     1741 aaatggccca cgcggtgcat cagcgcggcg gccttatctt ccacgatgtg accaacctgc
     1801 gcttcgcccg caaggcggca gaggccggtg tcgacgggct gatctgcatc ggcggcggcg
     1861 gcggcggcca ttcgggcacg ctcaacccct ttgccttcat ccccaaggtg cgcgccttct
     1921 tcgacggggt gatcgtctat gccggctgcg tcaccgatgg cgcggccatc cgcgcggccg
     1981 aagtgctggg cgccgatctc gcctatgtcg gcacgcgctt catcgccacc tgcgaggccc
     2041 gcgcgcccga ggcctacaaa cagatgctga tcgccggcgg cgcggaggat ctgcgctatt
     2101 ccccgggcat caccggggtc aacgccaact ggctggccgc ctcgctggaa cgctgcgggg
     2161 tcgatctcga agcgctcggc gccttcagcc gcgattatgc ccatctcccc cccgacatcc
     2221 gcccctggcg cgatgtgtgg agcgccggcc agggcgtgga actgatcgag gattgcccgg
     2281 atgcggccag ccttgtcgcc cgtttgcgcc gcgaatatgt ggctgcctgc gccgtgccgg
     2341 atctggccgc cagcgcccgg caggcggagc cggcgctgtg accccggccg atctgctggc
     2401 cggcgccgac agccccctgg tcatcgccga caggggcggg ctgcgctttg tgttgctcaa
     2461 ccgccctgcc gcgcgcaatg ccctcacccg cgcgatgcgg gccgatttcg cccgcctgct
     2521 ggccgaggcg gaagccgacg acagcgtgac ggcgctggtg ctgaccggcg ccggcggctg
     2581 tttcagcgcc ggggtggacc tcaaggaccg gatccccggc gcagcgccc
//
LOCUS       DASRYE010000296        29955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_261514,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000296 DASRYE010000000
VERSION     DASRYE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 29955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29955
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261514"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFF98_15155"
     CDS             complement(<1..225)
                     /locus_tag="VFF98_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZU65022.1"
                     /translation="MLEYDQIDRAQVPGGVELQLLRSGDDFAIVFGQNELMSSDMFAS
                     EEALATLTCERIGGQAAPQLLIGGYGMGFTL"
     gene            complement(225..698)
                     /locus_tag="VFF98_15160"
     CDS             complement(225..698)
                     /locus_tag="VFF98_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HZU65023.1"
                     /translation="MPATRQILVDADACPVKEEIYKVAFRHQAPVTIVSNQRLRVPDH
                     PLVRRVVVGDGFDAADDWIAQASHPASVVITADILLADRAIKAGAMVLAPTGKPFTAS
                     SIGGAIATRAIMADLRAGAMGEALGGPAPFSKADRSRFLSALDEALTRLARGRPA"
     gene            1070..1276
                     /locus_tag="VFF98_15165"
     CDS             1070..1276
                     /locus_tag="VFF98_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZU65024.1"
                     /translation="MPIGTVKFFNTDKGYGFIAPENGGDDSFVHISAVQAAGMATLNK
                     DQRLSYEIETDRRGKQSAVNLVSA"
     gene            complement(1486..1806)
                     /locus_tag="VFF98_15170"
     CDS             complement(1486..1806)
                     /locus_tag="VFF98_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65025.1"
                     /translation="MLYKNGRGGTVEVDGFKYDWELQSEPQLSSFDGWKGMTVSLRQQ
                     GMPREAVLEFPTPKRLLKGLPKGRSHINDTIAARGVRAALSAGWDPASRGKPTTFMVD
                     ANGD"
     gene            complement(1885..2712)
                     /gene="rlmJ"
                     /locus_tag="VFF98_15175"
     CDS             complement(1885..2712)
                     /gene="rlmJ"
                     /locus_tag="VFF98_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZU65026.1"
                     /translation="MNYRHSFHAGNSADVVKHCLLMALVRALQQKEGALTLIDTHAGC
                     GLYDLYGDEAQRTGESAQGVLRAFADTNPLLDDYRAAVQAVNSGEEPRLYPGSPRFLA
                     QLLRPQDLLILNEKHAQDAYALRGAMRDTQAAVHERDAYELWLAMVPTRTPRGVVVVD
                     PPYEQTDERARITATLAAAHRKWAHGVTVIWYPLKERPMHAQWKHQLRKLGIPKFLNV
                     EHWLYDADQPGIYNGAGLFIINPPYAFTQELPPLLEALRAALAPEGHRGEITADWLT"
     gene            complement(3100..4503)
                     /locus_tag="VFF98_15180"
     CDS             complement(3100..4503)
                     /locus_tag="VFF98_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU65027.1"
                     /translation="MLRQMSVLITSASLLGACAVGPDYRPPATPKAAAAPFQAATPAV
                     SAAAPEDRWWALYHDPVLDRLVADALAANTDIRVAVARLERARAALRAGKADRLPQTT
                     LGASADYGRASLASTLPGYSRQTWTYDGGISLAYEADLFGRVSRGVEAARADASAAAA
                     DAASVRLQVAADTVRAYLDAITSAERMAVARQTVALLDSSIRITSARFEVGRSDRLDV
                     IRVTALRDQQAATIPTLEADHEAALLRLATLTGRAPADLPADIARQQATPGLAAPIPV
                     GDGRQLLARRPDVRAAERRLAAASARIGIATADLYPRITLGGSAGATALSGMDVLGAG
                     AARWAVGPAISWAFPNFAAVHARIGAARADDAAALASFDGTVLNALEETERALSAYGH
                     ALEREETLGAARDAAARAARISLARQREGRIDFLDLLDAQRTLASAEADLVAARRARA
                     FAQVDVFRALGGGWNAS"
     gene            complement(4517..7693)
                     /locus_tag="VFF98_15185"
     CDS             complement(4517..7693)
                     /locus_tag="VFF98_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZU65028.1"
                     /translation="MRLSRFFIDRPIFAAVIAVVITVVGALAFIGLPVSQYPDIVPPT
                     VTVTAQYPGASAETVAATVAAPIEQEINGVDNMLYMSSQSTGDGKLTITVTFRIGTNL
                     DAAQVLVQNRVAVATPRLPDEVQRLGIVTRKSTPDFLMVVNLQSPDGSLDRNYLSNYA
                     LTQVKDRLARLDGVGDVQLFGSRDYAMRVWIDPGRAAALNLTAGEIVAALRAQNVQVA
                     SGAIGQPPYDKGNGAFQLGVEMQGRLASPDQFADIVIRTDADGHQVRVRDVARVELGA
                     QDYATNTYLSNKPTVVVAVMQRPGSNALDAAAHVKAEMDALSRQFPRGLEYSVIYNPT
                     EFISQSIDAVYHTLFEAVVLVVLVILVFLQNWRAAIIPIIAIPVSLIGTSAMLAALGY
                     SLNNLSLFGMVLAIGIVVDDAIVVVENVERNIEHGLSPLQAARVSMDEVSAALVAIVL
                     VLCAVFVPTLFITGMSGAFYKQFAVTISTATVISLVLSLTLSPAAAALLLREKAPLAA
                     NAPRWQRLLARAGDGFNTGFERLGAAYARLTRRLLARPKQMLLAYGGLIAATIALFVA
                     TPAGFIPAQDQGYFLAIVQLPAGASLERTDAVTREVAAKILPIQGLRGAVMFAGFHGP
                     SQTTAPNSAAIYFPFKTFAERKALGVTYAGIMDQAQKAVAGYDKARIILAPPPVIQGI
                     GSAGGYRYMLEDRADQGYDALNRASEGFIGKANATPGLAQVYTLFDVGTPRIFADVDR
                     RKADLLGVPPERVFEAMQVYLGSSFVNDFNLLGRTYRVTAQADAAHRDSVADIADLKT
                     RSNTGQMVPIGSVATFRDKTGPYRVVRYNLMPAVEVDGDVAPGHSTGQALTTIEKLAD
                     ASLPAGYAGEWTGIAYQQKTAGNTAGVVFAMAVFFVFLVLAAQYESLTLPLAIILIVP
                     MCLFAAMLGVNLRGMDNNILTQIGLVVLIALAAKNAILVVEFAKQAEEEQRLSPVEAA
                     VFAARARLRPILMTSLAFILGAVPLVIASGAGAELRQALGTAVFFGMTGVTGFGLLFT
                     PTFYVLCRKLALAAARRRPAAPALAPAE"
     gene            complement(7715..8878)
                     /locus_tag="VFF98_15190"
     CDS             complement(7715..8878)
                     /locus_tag="VFF98_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU65029.1"
                     /translation="MVLAGLADAGWKMLAPPPAAAIAPEPTLGVARPLLRPVVQWDDY
                     VGRFAPSQMVEVRPRVSGQITALHFHDGDIVHKGQLLFTIDQRPFQAALAEARANVAS
                     AQATLTLARADAARVSRLSGDEAISAAEIDALRARVQSASAALAAAQARARSRALDMG
                     FTEVRAPITGRVSDRRVDVGNLVAAGDGAGATLLTTINALDPIYFTFDASEALFLKNQ
                     RAKAGENGEVEIRLQDEGSYRWKGRLDFTDNGLDPHSGTIRGRAVLANPGLFLAPGLF
                     GNMRLANGGKVPTLLVPDAAIVPDQARKTVLVVGADGTVAARPVELGPLVDGLRIIRS
                     GLSAQDRVVVSNLQAASPGAHVRTRPATIAPDSAAPPAPAASTPAAAQATFAR"
     gene            complement(9410..10018)
                     /locus_tag="VFF98_15195"
     CDS             complement(9410..10018)
                     /locus_tag="VFF98_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU65030.1"
                     /translation="MECAPSPVRGRPRQFDPEQALAAALQVFWRKGYEGASLSELTEA
                     MGITRPSLYACFGNKEALFRKALDLYERDKLCYMRSALQAPTARGVAQRLLEGSLALQ
                     TGQSDPRGCLGVISAVACTTYNETMKDEVMARIAQSDEELLQRMIRAREEGDFGAGHD
                     PVALVFFLKAVLQGMGVQASLGASPAQLRHLMETTLALWPGR"
     gene            complement(10081..10629)
                     /locus_tag="VFF98_15200"
     CDS             complement(10081..10629)
                     /locus_tag="VFF98_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZU65031.1"
                     /translation="MLAALAGCDRQSGPAAQPSAAASAEGGAPGAEGATPGIDTSHKG
                     SRLPDLTFKDPQGGEIRTATLAGKPLLINLWATWCGPCVAELPTLDALAQQRGGALTV
                     LAISEDSAPAATVAAFLKAHSASHLVAALDPDTAASSQWQAQSLPTTIYYGADGREVW
                     RSTGGMDWTGPAATALLARAAS"
     gene            10697..12070
                     /gene="argH"
                     /locus_tag="VFF98_15205"
     CDS             10697..12070
                     /gene="argH"
                     /locus_tag="VFF98_15205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZU65032.1"
                     /translation="MWGGRFAEGPSAIMREINASIPFDKALWQQDIRASKAHVAMLGA
                     QGIVSAGDAATISAGLDQVAGEYAASGVPENWDLEDIHMTTESRLAELVGPVAGRLHT
                     ARSRNDQVATDFRLWVRDAMDEALAGLNALQKALVTRAGEHAASIMPGFTHLQTAQPV
                     TLGHHLMAYYEMAARDVSRFADARARANQCPLGSAALAGTGFPIDRFATAAALDFARP
                     TNNSLDSVSDRDFALDYLMAASQCALHLSRLAEEFIIWASQPFGFVTLPDSLSTGSSI
                     MPQKKNPDAAELVRGHSGRIIGSLTSLMITMKGLPLAYSKDMQDDKPPVFEAAGLLTL
                     CLAAMTGMVERTTFRTARMRQAAELGYATATDLADWLVRQANIPFREAHHITGAAVKL
                     AEKQGIALDQLRLEELQAIDPRITAAVYPALSVEASVASRASHGGTAPAEVEKRVAEA
                     RAALGLA"
     gene            12139..12390
                     /locus_tag="VFF98_15210"
     CDS             12139..12390
                     /locus_tag="VFF98_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65033.1"
                     /translation="MRQLLPCCAALAALLALSACGSQADLRPRAGHQLPVAPYGRTDR
                     PGSGELLGVGSQARPAHNVELHTKSELRADDPFDLPPQD"
     gene            12426..13685
                     /gene="lysA"
                     /locus_tag="VFF98_15215"
     CDS             12426..13685
                     /gene="lysA"
                     /locus_tag="VFF98_15215"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZU65034.1"
                     /translation="MDHFTYREGELYAEDVAVAAIARAVGTPVYIYAQATLERHARVF
                     AAALSALPKKHIAFAVKSNPNLAVLRVLAREGYGADVVSAGEMRRALAAGMPAADIVF
                     SGVGKTRAELIEALEAGIGQFNLESEEEGVELAALAAARGLKAPAVLRVNPDVDAGTH
                     AKISTGKAENKFGVAYDRAAAIYGRLAILPGLAMRGLACHIGSQISNLEPSRAAFTRM
                     GLLMDEIRAAGHPVTHMDLGGGLGVPYRAGEVLPEPAAYGAMVAEVTAGWDVTVMFEP
                     GRVITGNAGILVTEVVRVKEGVSAPFVVVDAAMNDLVRPAMYEAWHDFAAVAPSGEKM
                     VANIVGPICETSDTFAMGREIDVVQAGDLAVFRTAGAYGATMANTYNSRALVPEVMVS
                     GGQWAIVANRIEPATILAAEQVPGWLA"
     gene            13685..14500
                     /locus_tag="VFF98_15220"
     CDS             13685..14500
                     /locus_tag="VFF98_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65035.1"
                     /translation="MLDSLPLFHRLAQRPVIVLGAGEAAQAKRRLVERAGAVVFTDMA
                     QGLAAGARLAFIAHETRAACAAEAARLTAAGVLVNVVDQPDLCDFTVPSLLDRAPVLV
                     AVGTGGASAGLAKALRLRLEMLLPQGLGDLARALGAARGALKARLPDNTARRQALDAA
                     LGPGGALDLLDGAAAARVAGWAAGDLAAPEALLVELHIPSADPEELTLRQARLLGSAD
                     GIAHEPGVAPAILHRARADAARRPIAAGEAVHQAPGAGLWLVLRSGVTSSTSC"
     gene            complement(14487..14972)
                     /gene="ruvX"
                     /locus_tag="VFF98_15225"
     CDS             complement(14487..14972)
                     /gene="ruvX"
                     /locus_tag="VFF98_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZU65036.1"
                     /translation="MAEGLITTGALAMRDALPEGGALIGLDLGTQTIGTAFCDAAWRI
                     ASPGKTIRRSKFSIDKALIAELIAERQVAGLVIGLPLNMDGSEGPRAQSARAYARNLS
                     VLGLPILLWDERWSTTSAERALIEQDMSRAKRKDRIDSAAAAVILQAACDALAGGFSS
                     W"
     gene            complement(14957..16165)
                     /locus_tag="VFF98_15230"
     CDS             complement(14957..16165)
                     /locus_tag="VFF98_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HZU65037.1"
                     /translation="MARKFLYAVALLVVVVIGALMGLRFWAKELSQFAFVPSARFAAL
                     APMAPAAWDDPALWISRAGGHPGDPARWLPAGASEVPELPAAVFFIHPTSSFDRTRWN
                     APVDDALAATRAEIFARGLASPFGAARELWAPRYRQATFGAFLTDRADGTQALEAAYA
                     DVLAAFDHFAAHADPALPIVLVGHSQGALHLMKLMQQRVAGKPLAARIAAAYVVGWPV
                     SLAHDLPEMGLPACTRSDEAGCVLSWQSFAEPADPSMVLDAYARLPGLDGKNRKGSPF
                     LCINPITGRPGDAAEGWANIGTLVPDATLAHGALMPGLVPARCAPNGFLLVGPPPQMG
                     PYVLPGNNYHVYDIPLFWANVRADVTRRVNSWQAAHRAPPTTAEGKTAAAETPAASRH
                     ARSWWPWRRG"
     gene            complement(16170..17294)
                     /locus_tag="VFF98_15235"
     CDS             complement(16170..17294)
                     /locus_tag="VFF98_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZU65038.1"
                     /translation="MSTPPRRRPPRAPLTRHRAGPTDIADPLIRQEAKRAMIWLGLAG
                     LIALAVVLAQSLMVVFGGMVFGALIDGGARVIGRVLPIGRGWRVALVLLAATLFTYWV
                     IVFAGNEIAGQAAALPSTLQSQGMHILHWAEHHGLAVNSHLTQQITDKLMASLDQLGG
                     VLGGLIGGVTNLVLIFVLGIYFAIDPAPYQRGLGWMLPKESRLFFDATFAQMGHAMRR
                     LLFGRLLGMAIEGLTIGLALALYGVPMASLLGLLAGLLAFLPNIGAPISGLLMVLVGF
                     SGGLHMGLYCIGVYIVVQGVDGNIIVPMVAKRSADLAPALVLSMQLIMGVLFGIIGLA
                     LADPLLAMIKVLLERLAARGDAADDTEAQSPADTAMADRE"
     gene            complement(17299..18159)
                     /gene="lepB"
                     /locus_tag="VFF98_15240"
     CDS             complement(17299..18159)
                     /gene="lepB"
                     /locus_tag="VFF98_15240"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZU65039.1"
                     /translation="MTTPMPSPPAPRTHWLSEIRGFGGMLLAVLAFHSLVAKPFYIPS
                     ASMLPNLWVGDHLVVNKFAYGWDWASPSFHVLPRGQWRVWPKTPEYGDIVIVVPRGRP
                     DEDYIKRVVALPGDRIAVDNGQIVLNGRRVPQAVEPAVDIPLDAIRSDEDPEPCTGWG
                     FDGMRQRRPSGQEVCEMPVLRETLPNGATYRIIDHMDEPLDHMAEITVPAGEVFLMGD
                     NRDHSADSRASLEERGLGGPVPIADIGGRAEFITHSFDGSAGWNPLSWLHSLRAGRAG
                     NTLRPALANR"
     gene            complement(18186..18587)
                     /gene="acpS"
                     /locus_tag="VFF98_15245"
     CDS             complement(18186..18587)
                     /gene="acpS"
                     /locus_tag="VFF98_15245"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZU65040.1"
                     /translation="MILAIGSDLCNIERIESALARHGARFLARVFTDVEQARADRRPF
                     TRAGTLAKRFAAKEAMAKALGTGFARGVFMKDIGVVNSASGAPTLALTGGAAARLAEI
                     TPAGHKALVHLTLTDDHPWAQAFVVIEAQAC"
     gene            complement(18584..19333)
                     /locus_tag="VFF98_15250"
     CDS             complement(18584..19333)
                     /locus_tag="VFF98_15250"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZU65041.1"
                     /translation="MAAPAGSAPGRLRLGVNIDHVATIRNARGGDHPDPVRAAAIVAA
                     VGGDGITAHLREDRRHIRDEDLARIQAATNLPLNLEMAATEEMLAIALRHRPHAACIV
                     PERREERTTEGGLDAAGQHNRLAPIVGRLLDAGIRVSLFIAPEPRQIEAAMKLAAPVV
                     ELHTGEYAHATGDAQAVELKRIADMAALAAKNGIEPHAGHGLTYENVQPIAAIPQLAE
                     LNIGHYLIGEAIFGGLEAAVRHMRALMDAAR"
     gene            complement(19336..19917)
                     /gene="pyrE"
                     /locus_tag="VFF98_15255"
     CDS             complement(19336..19917)
                     /gene="pyrE"
                     /locus_tag="VFF98_15255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZU65042.1"
                     /translation="MTEDEVLAEFRASQALLEGHFLLSSGRHSAHYLQCARVLMDPMR
                     ASRLAAAIAAKIPRELRKEITRVVSPAMGGIIIGHEMGRALGLEAMFVERPTGTFELR
                     RGFALTPGDKVLMVEDVVTTGLSSREAIKAVEEAGGKVIAAASLVDRSGGTADLGVPF
                     FPLIQLSFPTYAPDEVPESLAATPAIKPGSRAR"
     gene            20212..21156
                     /gene="coxB"
                     /locus_tag="VFF98_15260"
     CDS             20212..21156
                     /gene="coxB"
                     /locus_tag="VFF98_15260"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZU65043.1"
                     /translation="MRRRSFGGLLLAGAALAAPFALPGSAWAAAADRFAVAPGGYSPM
                     KPTPGKGMPVDGGITFQTQYSPNGQYALWMHNQILLYIVVFTSVFVLGLLLWVIVRYN
                     SRANPVPSKTTHNTLLEVVWTGIPILILAVVFVPSIKLLAQQYHPAPAGALTVKATGN
                     QWYWSYSYPDNGGFEVTSNILKPEEAIARGEPPQLAADNRMVVPAGEPIRLQTFGADV
                     IHSFAVPSLWFKLDAVPGRINEKVLFIKEPGVYYGQCSELCGVRHGYMPIVVEALDRP
                     HFNAWVLTHSGAKVDGAPQAAAPAAPDSAAPAAAASGV"
     gene            21184..22872
                     /gene="ctaD"
                     /locus_tag="VFF98_15265"
     CDS             21184..22872
                     /gene="ctaD"
                     /locus_tag="VFF98_15265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZU65044.1"
                     /translation="MASIASEFQTDGPHGHTHGEHEHKPGFFARWFMSTNHKDIGTLY
                     LIFAVFAGLVGGAISGLMRAELAEPGIQYLGAIADFLGVKNPSFDQQLHLWNVLITAH
                     GLIMVFFMVMPALIGGFGNWFVPIMIGAPDMAFPRMNNISFWLTVAGFCSLMMSAFVP
                     GGTSNGAGIGWTAYAPLSTTGSQGPAVDFAIFSLHLAGAGSIMGAINFITTIFNMRAP
                     GMTLHKMPLFVWSVLVTAFLLLLALPVLAAAITMLITDRNFGTTFFDPAGGGDPILYQ
                     HLFWFFGHPEVYIMILPGFGIISQIVSTFSKKPVFGYLGMAYAMVAIGVVGFVVWAHH
                     MYTTGLSEDVKMYFTTATMVIAVPTGIKIFSWIATMWGGSITFRSPMVWAIGFIFLFT
                     VGGVTGVVLANGGIDDNLQDTYYVVGHFHYVLSLGAVTSLFAGFYYWFPKMSGRWHSE
                     LLSYLHFAVFFVGVNMIFFPMHFLGMQGMPRRYPDYAPAYAHWNHIASLGYHIMAASI
                     VIWLVNVVYGLFFGRKAEGNYWGEGATTLEWTLTSPPPFHQFETLPVIAEEPHH"
     gene            22956..23885
                     /locus_tag="VFF98_15270"
     CDS             22956..23885
                     /locus_tag="VFF98_15270"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZU65045.1"
                     /translation="MSAPLHAPAAAAAPLPADWRDFFALTKPRVMTLVIFTGLCGLLA
                     APGHVHPVIAFTAILCIAVGAGGAAALNQWWEADIDAGMKRTAKRPLPAGRMHRDSAR
                     DFGVGLSFAAVITMGLGVNWLCAAILAVSIVYYAVLYTIWLKPRTPQNIVIGGGAGAF
                     PPLIGWVAVTGHVTLMPVLLFALIFMWTPPHFWSLALFVQTDYAKVGIPMLPVVAGEP
                     ATRRQILIYSILLLPLSLVPWWIGGAGAIYGWSAAVLSGLFLALAVRVGLRRRAPEDD
                     SMKPEKQMFAYSVLYLFVIFAALVADRIWAGWK"
     gene            23887..24027
                     /locus_tag="VFF98_15275"
     CDS             23887..24027
                     /locus_tag="VFF98_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65046.1"
                     /translation="MSDGPEPVDIAAERRRIIAARNKVLALVLLGFVVLFFAITIAKM
                     QH"
     gene            24032..24601
                     /locus_tag="VFF98_15280"
     CDS             24032..24601
                     /locus_tag="VFF98_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZU65047.1"
                     /translation="MAASSPALAAQRNRRVGLAAALAAAGMLGLGYAAVPLYRLFCQA
                     TGYGGTPARASEAQAAGVRAIGRMISVRFDANVDAGMGWAFAPEQTTISLPIGQRRLA
                     FFTAENRTGHTITGAASYNIAPEEAARYFTKVQCFCFTRQVLKPGEKVRMPVVFYVNP
                     AILDDADVRDIQQLTLSYTFHPAAGAQTP"
     gene            24723..25544
                     /locus_tag="VFF98_15285"
     CDS             24723..25544
                     /locus_tag="VFF98_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZU65048.1"
                     /translation="MAGAKSHDYHILPPSIFPLLGAFSALTMTSGGVLFMHGTAGGKQ
                     VLLAGLFGVLLTMFLWWTSVVHEAHQGHHTPVVQLHLRYGMILFIASEVMFFVGWFWA
                     FFDFSLFPSTIAKVVGGHWPPVGIDKVLDPFDLPLLNTLILLCSGTTVTWAHHGLIHG
                     DRGALKKGLLLTIVLGLLFTSLQAFEYAHAPFAFGKNTYGSAFYMATGFHGLHVIIGT
                     IFLTVCLFRAYRGDFTPRQHFGFEAAAWYWHFVDVVWLFLFVCIYVWGNWGFPVE"
     gene            25608..25952
                     /locus_tag="VFF98_15290"
     CDS             25608..25952
                     /locus_tag="VFF98_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HZU65049.1"
                     /translation="MIPAALFGLCPRCGAKGLFAGLARFAPACRRCGLDFSGFNVGDG
                     PAAFLTFGVGALVVVVAAWLALSVDPPWWVYVLVLVPLTFGPTLWGLRLGKAALLVAE
                     YRRKAGEGRLKE"
     gene            25949..26560
                     /locus_tag="VFF98_15295"
     CDS             25949..26560
                     /locus_tag="VFF98_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZU65050.1"
                     /translation="MSRRVPVIPTLVVLLAVALMLRLGVWQLDRLREKEAMIARYQAA
                     LALPALDWAEGVPLGAAPAYRRVRLACQHFGQPRMVGGRNGRGVAGWALWADCTAPSG
                     SITVVAGWSARPETAALPGAAGVSGIVLVGKGGEMRLVADPPLAGLAANARPDPHDLP
                     NNHFSYAMQWFAFAATALVIYGLALRARWRRGASDFGGASGSA"
     gene            26631..28031
                     /gene="thrC"
                     /locus_tag="VFF98_15300"
     CDS             26631..28031
                     /gene="thrC"
                     /locus_tag="VFF98_15300"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZU65051.1"
                     /translation="MRYISTRGNAPALDFEGATLAGLASDGGLYVPESWPRFSAAEIA
                     ALAGLPYAELAARIMQPFVGESLPYERLLELTRAAYGRFAHAAVTPLKQFDEQHWLLE
                     LFHGPTLAFKDVALQLLGLFFEEFLARGSQNLTIVGATSGDTGSAAIDAVAGRAKVDI
                     FMLHPKGRVSDVQRRQMTTVLAPNVHNIAITGTFDDAQAMVKRMFNDEAMTGRFAIGA
                     VNSINWARLMAQVVYYFAAALQLGAPHRKVAFAVPTGNFGDVFAGYVAAQMGLPIERL
                     IVATNVNDILHRALATGDYSAGTVTPTAAPSMDIQVSSNFERLLFDVGGRDGAALAAQ
                     MAGFEATKAMTLTNAQREGAAALFSSARADADDMARAIRWAWDAAGELIDPHTAIGLH
                     AARAAGVPREVPVVTLATAHPAKFPEAVERSTGHRPALPERVGDLFEREERCADLPGD
                     YAAIADYVAAHATPKA"
     gene            28032..28940
                     /locus_tag="VFF98_15305"
     CDS             28032..28940
                     /locus_tag="VFF98_15305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU65052.1"
                     /translation="MAALSLQPLLLTGEGWADYGLVDSGAGRKLERYGPMRFIRPEPQ
                     ALWQPRLADWQAQGEFVPGADEDGGGRWHFEAPVPGEGWPLAWANGEDVVRFTAQCTP
                     FRHLGFFPDMAPVWGWMGEQLAGRSDASCLNLFGYTGVGTLALSRFGAVTHVDASKKS
                     VAQARANAALSGAEQRPIRWIVDDAAKFAAREVRRGKRYDGIILDPPKFGRGPEGEVW
                     RLEEHLPGLIGDCRRLLDADSRFLFLTVYAVRMSSLAIAGLLAEAFADLPGTIEHGDL
                     AVREDAREGAEGRLLPTAIFARWRAG"
     gene            29046..29417
                     /locus_tag="VFF98_15310"
     CDS             29046..29417
                     /locus_tag="VFF98_15310"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZU65053.1"
                     /translation="MADSLILEVADFESDVLTGIYSEETGKAQPLRITVAVRLTPAPR
                     YAPDTALSASKNYMDLKFAASQALPEGLHFTLIEAVADHICETLFLQDQRVEAVTVKI
                     VKLALSEKGEKIGVTLTRERR"
     gene            29417..29740
                     /locus_tag="VFF98_15315"
     CDS             29417..29740
                     /locus_tag="VFF98_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65054.1"
                     /translation="MELRRIVDLPDDPLAAAAAFHALHLPALRAATGDVAILFAPAEH
                     THAGWRLAAVQALARAATPRRVNAIEGAGEAAIAAACAWLAAAPGVTGQILRLAGGAG
                     AGVIG"
     gene            29745..>29955
                     /locus_tag="VFF98_15320"
     CDS             29745..>29955
                     /locus_tag="VFF98_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444584.1"
                     /note="together with moaC, is involved in the conversion
                     of a guanosine derivative (GXP) into molybdopterin
                     precursor Z; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZU65055.1"
                     /translation="MGAAPPLVDGLRRRITYLRLSVIDRCDLRCTYCMPERQQFLPRA
                     EVLSLEELHRLALAFIARGVTKLRLT"
BASE COUNT         4670 a         9874 c        10447 g         4964 t
ORIGIN      
        1 cagggtaaag cccatgccat agccgccgat cagcagttgt ggcgcggcct gcccgccaat
       61 cctttcacag gtcagcgtgg ccagcgcctc ttccgaggcg aacatgtcgc tgctcatcag
      121 ctcgttctgg ccgaagacga tggcaaaatc atcgccgctg cgcagcagtt gaagctcgac
      181 cccgccaggc acctgcgccc ggtcgatctg atcatattcc agcatcatgc cggccgccct
      241 cgcgccagac gggtcagggc ttcgtccagc gcggaaagga aacgcgaacg gtccgccttg
      301 gaaaacgggg ccgggccgcc cagcgcctcc cccatggcgc ctgcgcgcag atcagccatg
      361 attgcgcgcg tggcaatcgc cccaccgatg gaactggcgg taaacggctt gccggtgggg
      421 gcaagcacca tcgcgcccgc cttgatcgcg cgatcggcca acaggatgtc ggcagtaatg
      481 accacgctgg ccgggtggct tgcctgtgcg atccagtcgt cggcggcatc gaaaccgtcg
      541 ccgaccacca cgcggcgcac cagcggatga tcgggcacgc gcaggcgctg gttgctgacg
      601 atggtgaccg gtgcctgatg gcgaaaggcg accttgtaga tctcttcctt caccgggcag
      661 gcatcggcat cgacaaggat ctggcgcgtg gcgggcacgc ccggagctgg ggtggggctg
      721 ggcagggaaa tggggatgaa actccggcag gcacgcgggt cgatccacga tgcgtcgcga
      781 aacttgccgg ttggtgatgc gcgtgtgaac gggcgaagtg cgatctggct tgatcgtaac
      841 gtccggctgc taggcgaatt ggcacaatct tgccacgata atcggcagcg ccagagcatg
      901 acgggcccaa attgactttt tcaaggcgat ggcttaaagc gcccatcgct aacgtcgcct
      961 gcgacggaat ttgactccga ttcgttcggt tatccttcct ttccatgcta ccagctccat
     1021 ccgcgctttt gcgggtgggt tctcttgttt tcgtgaaaag gaaacaccca tgcccatcgg
     1081 caccgtcaag ttcttcaaca ccgacaaagg ttatggtttc atcgcgcccg aaaatggcgg
     1141 cgatgacagc ttcgtccaca tcagcgccgt gcaggccgcc ggcatggcga cgctgaacaa
     1201 ggatcagcgc ctgagctacg agatcgaaac cgaccgtcgc ggcaagcagt ccgcggtcaa
     1261 tctggtttcg gcctgaaccg atgttcgccg gcgtccgcgg ggcgccggaa acgctccgcc
     1321 gcgccgcaat ttccttgccg cgcggcggcc tgcgttcggt aagccaggcg ccggggcaat
     1381 caggatgtcc agggccgccg gcagggcctt ctgccaagcc tgcgcaaggc agagtttgcg
     1441 cgatgccgaa acccgccatt cgggctgtgc cgcgcgcccg cccgactagt cgccgttggc
     1501 gtcgaccatg aaggttgtgg gcttgccccg cgatgccgga tcccatcctg ctgacaaggc
     1561 cgcccgcacg ccgcgcgcgg caatcgtgtc attgatgtgg gaacgccctt tcggcaatcc
     1621 tttcaacaag cgcttgggcg tcggaaattc cagcacggct tcgcgtggca tgccttgctg
     1681 gcgcaaggag acggtcatgc ccttccagcc atcgaaggag gatagctggg gctcgctctg
     1741 gagttcccag tcatatttga aaccgtcaac ttcgacggtg ccgccacggc cgttcttgta
     1801 aagcatggca tgcctttacc acgccggcag cggcatgcca gccttcactg gtgccgcggg
     1861 gctttcccgg cccccttcat ctgcctaggt caaccaatcg gcggtgatct cgcccctgtg
     1921 cccctcgggt gccagcgcgg cgcgcagggc ttccagcaga ggcggcagtt cttgcgtgaa
     1981 ggcgtagggc gggttgatga taaacaggcc cgcgccgtta tagatgccgg gctgatcggc
     2041 atcatacagc cagtgctcca cgttcaggaa tttcgggatg ccgagcttgc gcagctggtg
     2101 cttccactgc gcatgcatgg ggcgctcctt cagcggatac cagatcaccg ttacgccatg
     2161 cgcccatttg cggtgagcag cggcgagggt cgcggtgatg cgcgcgcgtt cgtcggtctg
     2221 ctcatacggc gggtcgacca ccaccacgcc gcgcggcgtt cgggtcggca ccatcgccag
     2281 ccacagctcg taggcgtcgc gctcatgcac ggcggcttgt gtgtcgcgca tcgcgccgcg
     2341 cagggcatag gcgtcttggg catgtttttc gttcaggatc agcagatcct gcgggcgcag
     2401 aagctgtgcc agaaaccgcg gcgagccggg gtagaggcgc ggttcctccc cgctattcac
     2461 cgcctgcacg gcggcgcggt agtcgtccag caaggggttc gtgtcggcaa aggcccgcag
     2521 cacgccctgt gcggattcgc cggtgcgttg cgcttcgtcg ccataaaggt cgtatagccc
     2581 gcagccggca tgggtgtcga tcagggtcag cgcgccctct ttttgttgca aggcgcgcac
     2641 aagggccatc agcaggcagt gcttcacgac atcggcgctg ttgccagcat ggaaggaatg
     2701 gcgataattc attgtgaatc aaaatcctga cgatctgctg gatgcgggaa ccgagtgcaa
     2761 ggcgatggaa gcaaccgatc cggcgctctt agcgagccgg acggaaacct tcaaagccct
     2821 ttggcgcaag gccccgaagg cacgccagcg cagccgggat gaacggcttt gaaaaaatag
     2881 ccaacgagca cagatcgcaa tctgcatcga aataggcgtt gtgacggagg aatgcctcct
     2941 cgatcagcgc ttgcaccaag tccttgccga ccccgcgccg atggcaggcg ggagcgacat
     3001 agaccgaatg ctcgaccgaa tgggcatagc ctggccaagg gcgaaaatcg ccataagagg
     3061 cacagcctgg cagctcttct ccctccctga cggcaaggat cagctggcgt tccagccgcc
     3121 gcccagggcg cggaacacat ccacctgggc aaaggcgcgc gcgcgccggg ccgccaccag
     3181 atccgcctcg gccgaggcca gcgtgcgctg ggcatcgagc aggtcgagga aatcgatgcg
     3241 cccctcgcgc tggcgggcaa ggctgatgcg cgcggcccgc gctgcggcat cgcgcgcggc
     3301 gcccagcgtc tcctcgcgtt ccagcgcatg gccataggcc gaaagcgccc gctcggtctc
     3361 ttccagcgcg ttaagcacgg tgccatcgaa actggccagc gctgctgcat catcggcccg
     3421 ggcggcgcca atgcgggcgt gcacggcggc aaaattgggg aaagcccagc tgatcgccgg
     3481 ccccaccgcc caacgcgccg cgcccgcccc cagcacatcc atgccgctga gtgcggtggc
     3541 cccggcagag ccgcccagcg tgatgcgcgg gtaaaggtcg gcggtggcga tgccgatgcg
     3601 cgcgctggct gcggccagcc gcctttcggc cgcgcgcaca tcggggcggc gggccagcaa
     3661 ctggcgcccg tcgcccaccg ggatgggggc ggcaaggccg ggggtggcct gctgccgggc
     3721 gatgtcggcg ggcaggtcgg ccggcgcgcg gccggtgagc gttgccaggc gcagcagggc
     3781 ggcctcgtgg tcggcttcca gcgtggggat ggtggcggcc tgctggtcgc gcagggcggt
     3841 cacgcggatc acgtcgagcc ggtccgaacg gcccacctcg aagcgggccg aggtgatgcg
     3901 gatcgaactg tcgaggaggg ccacggtctg ccgggcaacg gccatccgct cggccgaggt
     3961 gatggcatcg agataggcgc gcacggtgtc ggccgccact tgcaggcgca cgctggcagc
     4021 atcggcggcg gcggcgcttg catcggcgcg ggccgcttcc accccgcggc tcacccggcc
     4081 gaacaggtct gcctcatagg ccaggctgat gccgccgtcg taagtccagg tctggcggct
     4141 gtagccgggc agcgtgctgg ccagcgaggc gcggccataa tcggccgagg cgcccagcgt
     4201 ggtttgcggc aggcgatcgg ccttgccggc gcgcagggcc gcgcgggcgc gttccagccg
     4261 ggccaccgcc acgcggatat cggtgttggc cgccagcgca tcggccacca gccgatccag
     4321 caccggatcg tgatagagcg cccaccagcg atcctccggg gcggcggcgg agaccgccgg
     4381 cgttgccgcc tggaacgggg cggcggcggc ctttggcgtt gccggcgggc gataatccgg
     4441 ccccacggcg caggccccca gcaggctggc gctggttatc agcacactca tctgtcgcag
     4501 catctcgttc ggttccttat tcggccgggg ccagcgcggg ggcggcaggg cggcggcggg
     4561 cggcggccag cgccagcttt cggcacagca catagaaagt gggcgtgaac agcaggccaa
     4621 agccggtaac cccggtcatg ccgaagaaca ccgccgtgcc cagtgcctgg cgcagttcgg
     4681 cgcccgcccc gctggcgatc accagcggca ccgcgcccag gatgaaggcc aggctggtca
     4741 tcaggatcgg gcgcaggcgg gccctggcgg caaacacggc cgcctccacc ggcgagaggc
     4801 gctgttcctc ctcggcctgt ttggcaaatt ccaccaccag aatggcattc ttggcggcca
     4861 gcgcgatcag caccaccagg ccgatctggg tgaggatgtt gttgtccatg ccgcgcaggt
     4921 tcacccccag catggcggca aacaggcaca tcggcacgat caggatgatc gccagcggca
     4981 gcgtcaggct ttcatattgc gcggccagca cgaggaagac gaagaacacc gccatggcaa
     5041 acaccacgcc tgccgtgttg ccggcggttt tctgctgata ggcaatgccg gtccattcgc
     5101 cggcatagcc ggctggcagg ctggcatcgg ccagcttctc gatggttgtc agcgcctggc
     5161 cggtggaatg gccgggggcg acatcgccat ccacttccac cgccggcatc agattgtagc
     5221 gcaccacgcg ataggggccg gtcttgtcgc ggaaggtggc gacagagccg atcggcacca
     5281 tctggccggt gttcgagcgg gttttcaggt cggcgatgtc ggccacgctg tcgcggtggg
     5341 cggcatcggc ttgcgcggtc acccggtagg tgcggccaag caggttgaaa tcattgacga
     5401 agctggagcc gagatagacc tgcatggcct cgaacacgcg ctctggcggc acgcccagca
     5461 ggtcggcctt gcggcggtcg acatcggcga agatgcgcgg cgtgccgaca tcgaacagcg
     5521 tgtagacctg ggccaggccg ggcgtggcat tggccttgcc gataaagccc tcagaggccc
     5581 ggttcagcgc atcatagccc tgatcggcgc ggtcttccag catgtagcga tagccgccgg
     5641 ccgagccgat gccctggatc accggcggcg gggccagaat gatgcgcgcc ttgtcgtagc
     5701 cggccacggc cttttgcgcc tgatccatga tgccggcata ggtgacgccc agcgccttcc
     5761 tttcggcaaa ggtcttgaag gggaagtaga tggcggccga attgggtgct gtggtttgcg
     5821 aggggccatg aaagccggcg aacatcaccg ccccgcgcag gccctggatg ggcaggattt
     5881 tggccgccac ctcacgcgtc accgcatcgg tgcgttccag cgaggcgccg gcgggcagct
     5941 gcacgatggc gaggaaatag ccctgatcct gcgccgggat gaagccggcg ggcgtggcca
     6001 cgaagagggc gatggtggcc gcgatcagcc cgccataggc cagcagcatc tgtttgggcc
     6061 gggccagcag gcggcgggtc agccgggcat aggccgcgcc cagccgttca aagcctgtgt
     6121 tgaagccatc gccggcgcgg gccagcaggc gctgccagcg cggcgcattg gccgccagcg
     6181 gtgccttctc gcgcagcagc agcgcggccg ctgccggcga cagggtgagg ctgagcacca
     6241 gcgagatcac cgttgcggtg gagatcgtca cggcgaactg cttgtagaag gcacccgaca
     6301 tgccggtgat gaacagcgtg ggcacgaaca ccgcgcaaag caccagcacg atcgccacca
     6361 gcgcggccga cacctcgtcc atcgacacgc gcgcggcctg cagtggcgag aggccatgct
     6421 cgatattgcg ctcgacattc tcgacgacga cgatcgcatc atccaccacg atgccgatcg
     6481 ccagcaccat gccgaacagc gacaggttgt tgagcgaata gcccagcgcc gccagcatcg
     6541 ccgatgtgcc gatcagcgag accgggatgg cgatgatcgg gatgatcgcc gcccgccagt
     6601 tctgcaggaa gacgaggatc accagcacca ccagcaccac tgcctcgaac agcgtgtgat
     6661 agaccgcatc gatcgactgg ctgatgaatt cggtggggtt gtagataacg ctatattcaa
     6721 ggccccgggg gaactgcctg gaaagcgcgt ccatctcggc cttcacatgg gcggccgcat
     6781 ccagcgcgtt ggagccgggg cgctgcatca ccgccaccac cacggtgggc ttgttcgaga
     6841 ggtaggtgtt ggtggcataa tcctgcgcgc ccagttccac gcgcgccaca tcgcgcacgc
     6901 gcacctggtg gccatcggca tcggtgcgga tgacgatatc ggcgaactgg tcggggctgg
     6961 cgaggcgccc ctgcatctcc acgcccagct ggaaggcgcc attgcccttg tcgtagggcg
     7021 gctggccgat ggcgccagag gccacctgca cgttctgcgc gcgcagcgcg gcaacgatct
     7081 cgccggcggt aaggttcagc gcggccgccc ggccggggtc gatccacacg cgcatggcat
     7141 aatcgcgcga accgaacagc tgcacatcgc ccacgccatc aaggcgcgcc agccggtctt
     7201 tcacctgcgt cagcgcatag ttcgacagat agttgcgatc gagcgagcca tcgggcgatt
     7261 gcaggttcac caccatcagg aaatccggcg tggacttgcg ggtgacgatg cccaggcgct
     7321 gcacttcatc gggcaggcgc ggggtggcca cggccacgcg gttctgcacc agcacttgcg
     7381 cggcatcgag gttggtgccg atccggaagg tcacggtgat ggtcagcttg ccatcgccgg
     7441 tggattggct gctcatgtag agcatgttgt cgacgccgtt gatctcctgc tcgatgggcg
     7501 cggcaacggt ggcggccacc gtttcggccg aggcgccggg atattgcgcg gtgaccgtga
     7561 ccgtgggcgg cacgatatcg ggatattggc tgaccggcag gccgatgaag gccagcgccc
     7621 ccaccacggt gatgaccacg gcgatgacgg cggcgaagat cggccggtca atgaagaagc
     7681 gggaaaggcg catgtgggct cctgatggga aggatcagcg cgcgaaagtg gcctgggcgg
     7741 cggccggcgt gctggcggca ggggcgggcg gggcggcgct gtcgggcgcg atggtggcgg
     7801 ggcgggtgcg cacatgggcg ccggggctgg cggcctgcag attgctgacc accaccctgt
     7861 cctgcgcgga aaggccgctg cggatgatgc gcaggccatc gaccagcggg cccagttcca
     7921 ccgggcgggc ggcaacggtg ccatcggcgc ccaccaccag caccgtcttg cgggcctgat
     7981 cgggcacgat cgcggcgtcg ggcaccagca gcgtgggcac cttaccgcca ttggccaggc
     8041 gcatgttgcc aaacaggccc ggcgccagaa acaggcccgg attggccagc accgcgcggc
     8101 cgcggatggt gccggagtgc gggtcgaggc cgttgtcggt aaagtccagc cggcccttcc
     8161 agcgatagct gccctcgtcc tgcaggcgga tttccacctc gccgttctcg cccgccttgg
     8221 cgcgctggtt cttgaggaac agcgcctcgg aggcatcgaa ggtgaaatag atcgggtcga
     8281 gcgcgttgat cgtcgtcagc agggtggcgc cggcgccatc gccggcggcc accagattgc
     8341 cgacatccac ccgccggtcc gacacgcggc cggtgatcgg cgcgcgcacc tcggtaaagc
     8401 ccatgtcgag cgcgcggctg cgggcgcgcg cctgggcggc ggcaagggcc gcgctggccg
     8461 attgcacgcg ggcgcgcagg gcatcgatct cggcggcgga aatcgcctcg tcgccgctca
     8521 gccggctgac gcgggcggca tcggcgcggg cgagcgtcag cgtcgcctgc gcgctggcga
     8581 cattggcgcg cgcttcggcc agggccgcct ggaaggggcg ctggtctatc gtgaacagca
     8641 gctggccctt gtggacgata tcgccatcgt ggaaatgcag cgcggtgatc tggcccgata
     8701 cgcgcgggcg cacttccacc atctggctgg gggcaaagcg gccaacataa tcatcccact
     8761 gcaccaccgg gcgcagcagc ggccgggcca cccccagcgt gggttcgggc gcgatggccg
     8821 cggcaggggg cggcgccagc attttccagc cggcatcggc aaggccggcc agcaccatca
     8881 gcgccagcgc gccatggcgc aggcgccagc ggcgcgatgg cgcggtttcg cgcaggggca
     8941 gggccggctc ggcagtggcg gcagggggca gaagcggggc aagctcggtc atgcaaaagg
     9001 gctccggcag gacaggaggg cgcaccaggc gctgccacat gaggtggcgg gaggaaggaa
     9061 ggacctgcgc gcacccggtg gggggcggga cggggcgcgc gcaggcccag ggatcggccg
     9121 ccggacgggg cggccgcggg aaaagggcaa gcccctgcct tgcggcgccg gcaggcgcca
     9181 ctgtgcaacg aggcgggcga ggggccgcgg caagcggcgc cgctccggcc gtgatcggca
     9241 agggcggcgc cgtgctgcgt ggtccgtcag ctggggtggt tggttggcaa agggggccgc
     9301 gcggggcggc gaaggctcag gaacaattca taccaagcgg tatatattgc gccgcagcac
     9361 ccgtcaaccc gctttctgta ccgcaaagta tggtaagggc cgccgcctgt cagcggcccg
     9421 gccaaagggc cagcgtggtt tccatcagat ggcgcagctg ggccgggctg gcgccaaggc
     9481 tggcctgcac gcccatgccc tgcaacaccg ccttcaggaa gaagaccagc gccacaggat
     9541 catgcccggc gccaaaatcg ccttcctcgc gagcgcggat catgcgttgc agcagttcct
     9601 cgtcagactg ggcgatgcgg gccatcacct cgtccttcat ggtttcgtta taggtggtgc
     9661 aggccaccgc gctgatcacg cccaggcagc cgcgcggatc gctctggccg gtttgcaggg
     9721 ccaggctgcc ttccagcagg cgctgggcca cgccgcgcgc ggtgggggcc tgcagggccg
     9781 agcgcatgta gcacagcttg tcgcgctcgt agaggtcgag cgccttgcgg aacagcgctt
     9841 ccttgttgcc gaaacaggca tagaggctgg gccgggtgat gcccatcgcc tcggtcagct
     9901 cgctcagcga ggcgccttca tagcccttgc gccagaacac ctgcagggcc gcggccagcg
     9961 cctgttcggg atcgaactgg cgcgggcgac cacgcacggg agagggagcg cattccatac
    10021 cgggcggtat atagcgattg cggggccgat cgtccagcag gcccgcccct gctgtttgta
    10081 tcaggaggcg gctcgggcca gcagggcggt ggcggcaggg ccggtccagt ccatgccgcc
    10141 ggtgctgcgc cacacttcgc ggccatcggc gccatagtag atggtggtgg gcagcgattg
    10201 cgcctgccac tggctggagg cggcggtgtc ggggtccagc gcggcgacaa ggtggctggc
    10261 gctgtgggcc ttgaggaagg ccgccacggt ggcggcgggc gcgctgtcct cagagatggc
    10321 gagcacggtg agcgcgcccc cgcgctgctg cgcaagagcg tcgagcgtcg gcagttcggc
    10381 cacgcagggg ccgcaccatg tggcccacag gttgatcagc agcggcttgc cggccagcgt
    10441 tgcggtgcgg atctcgcccc cttgcggatc cttgaaggtg agatcgggca ggcggctgcc
    10501 cttgtggctg gtgtctatgc ccggcgtggc gccctcggcg ccgggcgcgc cgccctcggc
    10561 gctggccgca gccgatggtt gcgcggccgg cccgctttgc ctatcgcacc cggcaagagc
    10621 ggccagcatc aggccacaac caaggacatt gcgcgtgagc ggacgggacg aaagcagcac
    10681 ttcaggctcc aaccagatgt ggggcggccg ctttgccgaa gggcccagcg ccatcatgcg
    10741 cgagataaat gcctccatcc ccttcgacaa ggccctgtgg cagcaggata tccgcgccag
    10801 caaggcgcat gtggcgatgc tgggcgcgca gggcatcgtc tcggccgggg atgccgccac
    10861 gatcagcgcc gggctcgatc aggtggcggg tgaatatgcc gccagcggcg tgcccgagaa
    10921 ctgggatctg gaagatatcc acatgaccac cgaaagccgg ctggccgagc tggtgggccc
    10981 ggtggccggg cgcctccaca ccgcgcgcag ccgcaacgat caggtggcca ccgatttccg
    11041 cctgtgggtg cgcgatgcga tggatgaggc gctggccggg ctcaacgccc tgcagaaggc
    11101 gctggtgacg cgggcgggtg aacatgcggc cagcatcatg cccggcttca cccatctgca
    11161 aaccgcccag ccggtcacgc ttggccacca cctgatggcc tattacgaga tggccgcgcg
    11221 cgatgtctcg cgctttgccg atgcgcgcgc gcgggccaac cagtgcccgc tgggcagcgc
    11281 ggcgctcgcc ggcaccggct tcccgatcga ccgtttcgcc acggccgccg cgctcgattt
    11341 cgcccggccc accaacaaca gcctcgattc ggtgtcggac cgcgatttcg ccctcgatta
    11401 cctgatggcc gccagccagt gcgcgctgca cctctcgcgc ctggcggaag aattcatcat
    11461 ctgggccagc cagcccttcg gctttgtcac cctgcccgat tcgctgtcca ccggcagcag
    11521 catcatgccg cagaagaaga accccgatgc cgccgagctg gtgcgcggcc atagcgggcg
    11581 catcatcggc agcctgacca gcctgatgat caccatgaag ggcctgccgc tggcctattc
    11641 gaaggacatg caggacgaca agccgccggt gttcgaggct gcgggcctgc tcaccctgtg
    11701 cctggccgcg atgaccggca tggtggagcg caccaccttc cgcaccgccc gcatgcgcca
    11761 ggccgccgag ctgggctatg ccaccgccac agaccttgcc gactggctgg tgcggcaggc
    11821 gaacatcccc ttccgcgagg cgcatcacat caccggcgcg gcggtgaaac tggccgaaaa
    11881 acagggcatc gcgcttgatc agctgcgcct ggaggaactg caggcgatcg atccgcggat
    11941 caccgcggcg gtctatcccg ccctgtcggt ggaagcctcg gtggccagcc gcgccagcca
    12001 tggcggcacc gcccctgccg aggtggaaaa acgcgtggcc gaagcgcgcg ccgccttggg
    12061 gcttgcctga ggcgccggcg ccggggcatg aagcctgtgc acgcaacccc gcgttccctt
    12121 gccgcaagga ggcacccgat gcgccagctt ctgccatgct gcgccgcgct ggccgcgctg
    12181 ctggcccttt ccgcctgcgg gtcgcaggcc gatctgcggc cgcgggcggg ccaccagctg
    12241 cccgtggccc cctatggccg caccgaccgg cccggatcgg gcgaattgct gggcgtgggc
    12301 tcgcaggccc gcccggcgca taatgtcgag ctgcacacca aatcggaact gcgcgccgac
    12361 gaccccttcg atctgccccc gcaagactga gcggggcgct tcaagaaccg acgaaaagag
    12421 tttcaatgga tcattttacc taccgcgagg gtgaacttta cgccgaggat gtggcggtgg
    12481 cggcgattgc ccgcgccgtg ggcacgccgg tctatatcta tgcccaggcc acgctggagc
    12541 gccatgcgcg cgttttcgcc gcggcgctgt cggcgctgcc gaagaagcat atcgcctttg
    12601 cggttaaatc gaaccccaat ctggcggtgc tgcgggtgct ggccagggaa ggctatggtg
    12661 ccgatgtggt gtctgccggc gagatgcggc gggcactggc ggcgggcatg ccggcggccg
    12721 atatcgtctt ttccggcgtt ggcaagacac gggcggagct gatcgaggcg ctggaagcgg
    12781 gcatcggcca gttcaacctc gaatccgagg aagagggcgt ggagctggcc gcgctggccg
    12841 ccgcgcgcgg gctgaaagcg ccggccgtgc tgcgggtgaa cccggatgtc gatgccggca
    12901 cccatgccaa gatctccacc ggcaaggcgg agaacaagtt tggcgtggcc tacgaccggg
    12961 ccgcggcgat ctatggccgg ctggccattc tgccgggcct ggcgatgcgg gggctggcct
    13021 gccacatcgg cagccagata tccaatctgg aacccagccg cgccgccttc acgcgcatgg
    13081 gcctgctgat ggacgagatc cgcgcggcgg gccatcctgt cacccatatg gatctgggcg
    13141 gcgggcttgg cgtgccctat cgcgccggcg aagtgctgcc cgagccggcc gcctatggcg
    13201 ccatggtggc cgaggtgacc gccggctggg atgtgacggt gatgttcgag ccgggccggg
    13261 tgatcaccgg caatgccggt attctcgtta ccgaagtggt tcgcgtgaag gaaggcgtga
    13321 gcgcgccttt cgtcgtcgtc gatgcagcga tgaacgatct cgtgcgcccg gccatgtatg
    13381 aggcctggca cgatttcgcg gcggtggcgc cgtcgggcga aaagatggtg gccaatatcg
    13441 tcggccccat ctgcgagacc tcggacacct ttgccatggg ccgcgagatc gatgtggtgc
    13501 aagccgggga tctggccgtt ttccgcaccg ccggcgccta tggggcgacc atggccaaca
    13561 cctataacag ccgcgcgctg gtgcccgagg tgatggtttc gggcgggcaa tgggcgatcg
    13621 tggccaaccg catcgagccc gccaccatcc tggccgccga gcaggtgccc ggctggctgg
    13681 cctgatgctg gatagcctgc ccctgttcca tcgcctggcg caaaggccgg tgatcgtgct
    13741 gggggcaggc gaggcggccc aggccaagcg ccggctggtg gaacgcgccg gtgctgtcgt
    13801 ctttaccgat atggcacagg ggctggccgc cggcgcgcgc cttgccttca tcgcccatga
    13861 aacgcgcgcg gcctgcgcgg cggaggccgc ccgcctcact gccgccggtg tgctggtgaa
    13921 tgtggtggat cagccggatc tgtgcgattt caccgtgcca tcgctgctcg atcgggcgcc
    13981 ggtgctggtg gcggtgggca cggggggggc ttctgcgggg ctggccaagg cgctgcgcct
    14041 gcggctggag atgctgctgc cgcaggggct gggcgatctg gcgcgggcgc tgggcgcggc
    14101 gcgcggggcc ctgaaggccc gcctgccaga caacaccgcc cgccgccagg cgctggatgc
    14161 ggcgctaggg ccgggcggcg cgctcgatct gctggatggg gcggcggcgg cgcgggtggc
    14221 cggctgggcg gcgggtgatc tggccgcgcc agaggccctg ctggtggagc tgcacatccc
    14281 ctcggcggat cccgaagagc tgaccctccg gcaagcgcgc ctgctgggca gcgccgatgg
    14341 catcgcccat gagccgggcg tggcgccggc cattctgcac cgcgcgcggg ccgatgccgc
    14401 ccgccggccg atcgccgctg gcgaggcggt gcatcaggcg ccgggcgcgg gcctgtggct
    14461 ggtgctgcgc agcggcgtta ccagctctac cagctgctga agccgccggc cagcgcatcg
    14521 caggcggcct gcaggatcac cgcggcggcg gccgaatcga tgcggtcctt gcgcttggcg
    14581 cggctcatgt cctgctcgat cagcgcgcgt tcggcgctgg tcgtgctcca gcgctcgtcc
    14641 cacagcagga tcggcaggcc cagcaccgac aggttgcgcg cataggcccg ggccgattgc
    14701 gcgcgcggcc cctcgctgcc atccatgttc agcggcaggc cgatgaccag gcccgccacc
    14761 tgccgttcgg cgatcagctc ggcgatcagc gccttgtcga tgctgaactt gctgcggcgg
    14821 atggtcttgc cggggctggc gatgcgccag gcggcatcgc aaaaggcggt gccgatggtc
    14881 tgcgtgccca ggtccagccc gatcagtgcg ccgccttcgg gcagggcatc gcgcatggca
    14941 agggcgccgg tggtgatcaa cccctccgcc atggccacca gcttctggca tgccgcgagg
    15001 ccgccggcgt ttcggccgcg gcggttttgc cctcggcggt tgtgggcggc gcgcggtggg
    15061 cggcttgcca gctgttcacc cgccgtgtca catcggcccg cacattggcc cagaacagcg
    15121 gaatatcata cacatgatag ttgttgccgg gcagcacata gggccccatc tgcggcggcg
    15181 gccccaccag cagaaagccg ttgggcgcac agcgcgccgg caccagcccg ggcatcagcg
    15241 cgccatgggc cagcgtggca tcgggcacca gcgtgccgat gttggcccag ccttcggcgg
    15301 catcgcccgg gcggccggtg atcgggttga tgcacaggaa cgggctgccc ttgcggttct
    15361 tgccatccag gccgggcagg cgggcatagg catccagcac catggaagga tcagccggct
    15421 cggcaaagct ctgccacgag agcacgcagc ccgcctcgtc ggagcgggtg caggccggca
    15481 ggcccatctc gggcagatca tgcgccagcg ataccggcca gcccaccaca taggccgcgg
    15541 caatgcgggc ggccagcggc ttgcccgcca cccgctgctg catcagcttc atcagatgca
    15601 gcgccccttg cgaatggccc accagcacga tcggcagggc cggatcggca tgggcggcga
    15661 aatgatcgaa agcggccagc acatcggcat aggcggcctc cagcgcctgg gtgccatcgg
    15721 cgcgatcggt gaggaaggcg ccgaaagtgg cctggcgata gcgcggcgcc cacagctcgc
    15781 gcgcggcgcc aaacgggctg gcaaggccgc gcgcgaagat ctccgcgcgg gtggcggcca
    15841 gggcatcgtc caccggcgcg ttccagcggg tgcggtcgaa cgagctggtg gggtggatga
    15901 agaacaccgc cgccggcagt tcgggcactt cggacgcgcc cgccggcagc cagcgcgccg
    15961 gatcgcccgg atgcccgccc gcgcgtgaga tccacagggc cggatcatcc cacgcggccg
    16021 gcgccatggg ggccagcgcg gcaaagcggg ccgagggcac aaaggcaaat tgcgacagtt
    16081 ccttggccca gaaccgcagc cccatcaacg cgccgatcac aaccaccacc aacagcgcaa
    16141 cggcatagag aaactttctg gccaaggggt cattcccgat ctgccatggc cgtatcggcc
    16201 ggtgattgcg cttctgtgtc gtcggcggca tcgccgcgcg cggccagccg ttccagcagc
    16261 accttgatca tcgccagcag cggatcggcc agcgccagcc cgatgatgcc gaacaacacg
    16321 cccatgatca gctgcatcga cagcaccagc gccggcgcca gatcggcaga gcgcttggcc
    16381 accatcggca cgatgatgtt gccatccacc ccctgcacca cgatatagac gccgatgcag
    16441 taaagcccca tgtgcagccc gcccgaaaag cccaccagca ccatcagcag ccccgagatc
    16501 ggcgcgccga tattgggcag gaaggccagc aggcccgcca gcagccccag caaagaggcc
    16561 atcggcacgc cataaagggc cagcgccagg ccaatcgtca gcccctctat cgccatgccc
    16621 agcaggcggc cgaacagcag gcggcgcatg gcatggccca tctgggcgaa ggtggcatcg
    16681 aagaacaggc ggctttcctt gggcagcatc cagcccaggc cgcgctgata gggcgccgga
    16741 tcgatggcga aatagatgcc cagcacgaag atcagcacca ggttcgtcac cccgccgatc
    16801 agcccgccca gcacgccgcc cagctggtcg aggctggcca tcagcttgtc ggtgatctgc
    16861 tgggtgaggt ggctgttgac cgccagcccg tgatgttcgg cccagtggag gatgtgcatc
    16921 ccctggcttt gcagggtgga gggcagggcg gcggcctggc cggcgatctc gttgccggca
    16981 aagacgatca cccaataggt gaacagcgtg gccgccagca gcaccagcgc cacccgccag
    17041 ccgcgcccga tcggcagcac ccggccgatt acccgcgcgc cgccatcgat cagcgcgcca
    17101 aacaccatgc cgccaaacac caccatcagc gattgcgcca gcaccaccgc cagcgcgatc
    17161 agcccggcca ggcccagcca gatcatcgcc cgcttggctt cctggcggat cagcggatcg
    17221 gcgatatcgg tggggccggc gcggtggcgg gtgagggggg cgcggggcgg gcggcggcgg
    17281 gggggcgtgc tcatgatcct agcggttggc cagcgccggg cgcaaggtgt tgccggcccg
    17341 gcccgcgcgc agcgaatgca gccagctcaa ggggttccag ccggcgctgc catcgaagga
    17401 atgggtgatg aattcggccc tgccgccaat gtcggcgatg ggcacagggc cgcccaggcc
    17461 gcgttcctca aggctggcgc ggctgtcggc ggaatggtcg cggttgtcgc ccatcaggaa
    17521 gacctcgccc gccggcacgg tgatttcggc catgtgatca agcggctcgt ccatatggtc
    17581 gatgatgcgg taggtggcgc cattgggcaa ggtctcgcgc agcaccggca tctcgcacac
    17641 ttcctggccc gaggggcggc gctggcgcat gccgtcaaag ccccagccgg tgcagggctc
    17701 ggggtcctcg tcggaacgga tggcatcgag cgggatatcc acggccggct ccaccgcctg
    17761 cggcacgcgc cggccattga gcacgatctg cccgttgtcc accgcgatcc ggtcgcccgg
    17821 cagggccacc acccgcttga tgtaatcctc atccggccgc ccgcgcggca caacgatgac
    17881 gatatcgcca tattccggcg ttttgggcca cacccgccat tgcccgcgcg gcagcacatg
    17941 gaaggagggc gaggcccaat cccagccata ggcgaatttg ttgaccacca gatgatcgcc
    18001 cacccacagg ttgggcagca tcgaggccga ggggatgtag aaaggcttgg ccaccaggct
    18061 gtggaacgcc agcaccgcca gcagcatgcc gccaaacccc cggatttccg agagccagtg
    18121 ggtgcgcggc gcgggcgggg agggcatcgg cgtagtcatg gaggaggtca tggggtcggt
    18181 tcggctcaac aggcttgcgc ctcgatcacc acaaaggcct gcgcccaggg gtgatcgtcg
    18241 gtcagggtca gatggacgag cgccttgtgg cccgccgggg tgatttcggc aagccgcgcg
    18301 gcggccccgc cggtcaacgc cagggtgggc gcgcccgagg cactgttcac cacgccgatg
    18361 tccttcatga acacgccgcg ggcaaagcct gtgcccagcg ccttggccat cgcttccttg
    18421 gcggcaaagc gcttggccag cgtaccggcg cgggtgaagg ggcggcgatc ggcccgggcc
    18481 tgttccacat cggtgaacac gcgggcgaga aagcgcgcgc catggcgggc cagcgcgctc
    18541 tcgatgcgct cgatgttgca caggtccgag ccgatggcga ggatcatcgc gccgcgtcca
    18601 tcagcgcgcg catgtggcgc accgccgctt ccagcccgcc gaagatcgcc tcgccgatga
    18661 gatagtggcc gatgttcagc tcggccagct gggggatggc ggcgatcggc tgcacgttct
    18721 cgtaggtgag gccgtggccg gcatggggct cgatgccgtt cttggcggca agcgcggcca
    18781 tgtcggcaat gcgcttcagc tccaccgcct gggcatcgcc tgtggcatgg gcatattcgc
    18841 cggtgtgcag ctccaccacc ggggcggcca gcttcatcgc cgcttcgatc tggcgtggct
    18901 ccggcgcgat gaacaggctg acgcggatgc ccgcatccag cagccggcca acgatcggcg
    18961 ccaggcggtt gtgctggcct gcggcatcca gcccgccctc ggtggtgcgc tcctcgcgcc
    19021 gttcgggcac gatgcaggcg gcgtgcggcc ggtggcgcag ggcgatcgcc agcatctcct
    19081 cggtggcggc catttccagg ttcagcggca ggttggtggc cgcctggatg cgggcgaggt
    19141 cctcgtcgcg gatatggcgc cggtcttcgc gcaaatgggc ggtgatgcca tcgccgccca
    19201 cggccgcgac gatcgccgcc gcgcgcaccg gatcgggatg atcgcccccc cgcgcgttgc
    19261 ggatggtggc gacatgatcg atgttgacgc ccagccgcag ccgcccgggc gccgatcctg
    19321 cgggcgccgc catgctcagc gcgcccggct gccgggcttg atcgccgggg tggcggccag
    19381 gctctcgggc acctcgtcgg gcgcataggt ggggaacgac agctggatca gcgggaagaa
    19441 gggcacgccc agatccgccg tgccgccgct gcgatccacc agcgaggccg ccgcgatcac
    19501 cttgccgccg gcctcttcca ccgccttgat cgcctcgcgg ctggacaggc cggtggtgac
    19561 gacatcctcc accatcagca ccttgtcgcc cggggtaagg gcgaatccgc ggcgcagctc
    19621 gaaagtgccg gtgggccgtt ccacgaacat cgcctccagc cccagcgcgc ggcccatttc
    19681 atggccgatg atgatgccgc ccatcgccgg cgaaaccacg cgggtgatct ccttgcgcag
    19741 ctcgcgcggg atcttggcgg cgatggcggc ggccagccgg ctggcgcgca tcggatccat
    19801 caacacgcgt gcgcattgca ggtagtgggc gctgtggcgg ccggaagaaa gcaggaaatg
    19861 gccctcgagc agcgcctggc tggcgcggaa ttcggccagc acttcgtctt cggtcatggt
    19921 cggatacacc tcgttgcgtt cgcggcgggc ctggcgctgc aggagcgcgt cattggccca
    19981 tgtcaaaaat tccccctaga ggcgcttgtg agccccggca agcgcgcgta taccttgatg
    20041 taaccggggt aaaaagcccc acctttcgct taggcggcgg ggaaaacggg ggggcggggt
    20101 gccgtctctc gtggaagtgg aagaggcaag gcaatgaatc tggacgatcg catccgcggc
    20161 gggggccgtg gcgtgtcagt tttgtgtctg ttatctggat tttctcgatc cttgcgccgc
    20221 cgatctttcg gggggctgtt gctggcgggc gccgcgctgg ccgcgccctt tgccctgccg
    20281 ggcagcgcct gggccgcagc ggcagaccgc tttgccgtgg cgccgggcgg ctacagcccg
    20341 atgaagccca ccccgggcaa gggcatgccg gtggatggcg gcatcacctt ccagacgcaa
    20401 tattcgccca atggccagta tgcgctgtgg atgcacaacc agatcctgct ctacatcgtt
    20461 gtgtttacca gcgttttcgt gctgggcctg ctgctgtggg tgattgtgcg ctataacagc
    20521 cgggccaatc ccgtgccttc gaagaccacc cacaacacgc tgctcgaagt ggtgtggacg
    20581 ggcattccga tcctcattct ggcggtggtc ttcgtgccct cgatcaagct gctggcgcag
    20641 cagtatcacc ctgccccggc cggcgcgttg acggtgaagg ccaccggcaa ccagtggtac
    20701 tggagctatt cctacccgga caatggcggc ttcgaggtga ccagcaacat tctgaagccc
    20761 gaggaggcca ttgcccgcgg cgagccgccg cagctggcgg ccgacaaccg catggtggtg
    20821 cccgccggcg agccgatccg cctgcagacc tttggcgccg atgtgatcca ttccttcgcg
    20881 gtgccctcgc tgtggttcaa gctggatgcg gtgccgggcc gaatcaacga gaaggtgctc
    20941 ttcatcaagg agccgggggt ctattatggc cagtgctcgg aactgtgcgg ggtgcgccat
    21001 ggctatatgc cgatcgtggt ggaagcgctg gacaggccgc atttcaacgc ctgggtgttg
    21061 actcacagcg gcgccaaggt ggatggggcg ccgcaagcag cggcccctgc cgcccccgat
    21121 tccgcggctc cggccgctgc cgcctccggc gtctgatcct ctcatcgaag gtgagctgac
    21181 cacatggcta gcatcgcatc cgaattccag accgatgggc cccacggcca cacccatggc
    21241 gaacatgaac acaagcccgg cttcttcgcc cgctggttca tgtccaccaa ccacaaggat
    21301 atcggcacgc tctatctcat cttcgcggtg tttgcggggc ttgtgggcgg cgccatttcg
    21361 ggcctgatgc gcgccgaact ggcggagccg ggcatccagt atctgggcgc catcgccgat
    21421 ttcctgggcg tgaagaaccc cagcttcgac cagcagctgc acctgtggaa cgtgctgatc
    21481 accgcccatg gcctgatcat ggtgttcttc atggtgatgc cggcgctgat cggcggcttt
    21541 ggcaactggt tcgtgccgat catgatcggc gcgcccgaca tggccttccc gcggatgaac
    21601 aacatcagct tctggctgac ggtggccggc ttctgctcgc tgatgatgag cgcctttgtg
    21661 ccgggcggca ccagcaatgg cgccggcatc ggctggacgg cctatgcgcc gctctccacc
    21721 accggcagcc agggcccggc ggtcgatttt gccatcttct cgttgcatct ggcgggtgcg
    21781 ggcagcatca tgggggcgat caacttcatc accaccatct tcaacatgcg cgcgccgggc
    21841 atgacgctgc acaagatgcc gctgttcgtg tggtcggtgc tggtgacggc gttcctgctg
    21901 ctgctggcgc tgccggtgct ggccgcggcc atcaccatgc tgatcaccga tcgcaatttc
    21961 ggcaccacct tcttcgatcc ggccggtggc ggcgatccca tcctctatca gcacctgttc
    22021 tggttcttcg gccaccccga agtctatatc atgatcctgc cgggctttgg catcatcagc
    22081 cagatcgtct ccaccttcag caagaagccg gtgtttggct atctgggcat ggcctatgcc
    22141 atggtggcga ttggtgtggt gggcttcgtg gtgtgggcgc accacatgta taccaccggc
    22201 ctgtccgaag acgtgaagat gtatttcacc accgccacca tggtgatcgc ggtgcccacc
    22261 ggcatcaaga tcttctcgtg gatcgccacc atgtggggcg gctcgatcac cttccgcagc
    22321 ccgatggtgt gggcgatcgg cttcatcttc ctgttcaccg tgggcggggt gacgggcgtg
    22381 gtgctggcca atggcggcat cgacgacaac ctgcaggaca cctattacgt ggtgggccat
    22441 ttccactatg tgctgagcct tggcgcggtc acctcgctgt tcgccggctt ctactactgg
    22501 ttccccaaga tgagcgggcg ctggcattcg gagctgctct cctatctcca tttcgccgtc
    22561 ttcttcgtgg gcgtgaacat gatcttcttc cccatgcatt tcctggggat gcagggcatg
    22621 ccgcggcgct atcccgatta tgcgccggcc tatgcgcact ggaaccacat cgcctcgctg
    22681 ggctatcaca tcatggccgc cagcatcgtc atctggctgg tgaatgtggt ctatggcctg
    22741 ttcttcggcc gcaaggcgga aggcaattac tggggtgaag gcgccaccac gctggagtgg
    22801 acgctgacca gtccgccgcc cttccaccag ttcgaaacgc tgccggtgat cgccgaagag
    22861 ccgcatcact gatggccctg gccgggggca tgcgcgccag cggggcttac gcgctccctt
    22921 tacaacgcgg cgaaggcgcg caaggggggt gggccatgag cgcccccctt catgcccccg
    22981 ccgccgctgc tgcccccctg cctgccgact ggcgcgattt cttcgcgctg accaagccgc
    23041 gggtgatgac gctggtgatc ttcaccggcc tgtgcggcct gttggcagcg ccgggccatg
    23101 tgcatccggt gatcgccttt accgccattt tgtgcattgc cgtgggggca gggggggcgg
    23161 ccgcgctcaa ccagtggtgg gaggccgaca tcgatgccgg catgaagcgc accgccaagc
    23221 gcccgctgcc cgccggccgc atgcaccgcg acagcgcgcg cgatttcggc gtgggcctgt
    23281 cctttgccgc ggtgatcacc atggggctgg gggtgaactg gctgtgcgcg gcgattctgg
    23341 ccgtctccat cgtctattac gcggtgcttt acaccatctg gctgaagccg cgcacgccgc
    23401 agaacatcgt catcggcggc ggggcggggg cgtttccgcc gctgatcggc tgggtggcgg
    23461 tgaccggcca tgtgacgctg atgccggtgc tgctgttcgc cctcatcttc atgtggacgc
    23521 cgccccattt ctggagcctc gcgctgttcg tgcagaccga ttacgccaag gtgggcatcc
    23581 cgatgctgcc ggtggtggcg ggcgagccgg ccacgcgccg gcagatcctc atctattcca
    23641 tcctgctgct gccgctcagc ctggtgccct ggtggatcgg cggggcaggc gcgatctatg
    23701 gctggtcggc ggcggtgctg tcggggctgt tcctggcgct ggcggtgcgg gtgggcctgc
    23761 gccgccgcgc gcccgaggat gacagcatga agcccgaaaa gcagatgttt gcctattcgg
    23821 tgctctatct cttcgtgatc tttgcggccc ttgtggcaga ccgcatctgg gcaggatgga
    23881 aatagcatga gcgacgggcc ggaaccggtt gatatcgcgg ccgaacggcg ccgcatcatt
    23941 gctgcgcgca acaaggtgct ggcgctggtg ctgctgggct ttgtggtgct gttcttcgcc
    24001 atcaccatcg ccaagatgca gcactgacgc catggccgcc tcttcccccg cccttgctgc
    24061 acagcgcaac cgccgggtgg ggctggcagc ggcgctggcg gcggcgggca tgctggggct
    24121 gggctatgcg gcggtgccgc tctatcggct gttctgccag gccaccggct atggcggcac
    24181 gcccgcccgc gcttcggagg cacaggcggc cggcgtgcgc gccatcgggc ggatgatctc
    24241 ggtgcgcttc gatgccaatg tcgatgccgg catgggctgg gccttcgcgc ccgagcagac
    24301 cactatcagc ctgccgatcg gccagcggcg cctggccttc ttcaccgccg agaatcgcac
    24361 cggccacacg atcaccggcg cggccagcta caatatcgcg cccgaggaag cggcgcgcta
    24421 tttcaccaag gtgcagtgtt tctgcttcac ccggcaggtg ctgaagccgg gcgagaaggt
    24481 gcgcatgccg gtggtgttct acgtgaaccc ggcgattctc gacgatgccg atgtgcgcga
    24541 tatccagcaa ttgacgctga gctatacttt ccaccccgca gcaggggcgc aaacgccatg
    24601 aaacggcagc ttgccggccc ataagccact ggaccgggcg ctgcttgcga gataggatca
    24661 cgatcaagaa aaagggagga aggcactgcc atttctcccg ccaaattggg gatgtctgaa
    24721 ccatggccgg agccaaatcg cacgattatc atatcctgcc gcccagtatc tttcccttgc
    24781 tgggcgcttt ctcggcgttg acgatgacca gcggcggcgt gctgttcatg catggcacgg
    24841 cgggcggcaa gcaggtgttg ctggcgggcc tgttcggcgt gctgctcacc atgttcctgt
    24901 ggtggacaag cgtggtgcac gaggcgcatc agggccatca cacgccggtg gtgcagctgc
    24961 acctgcgcta tggcatgatc ctgttcattg cttctgaagt gatgttcttc gtcggctggt
    25021 tctgggcctt cttcgatttc tcgctgtttc cttccaccat tgccaaggtg gtgggcgggc
    25081 actggccgcc ggtggggatc gacaaggtgc tcgatccttt cgatcttccc ctgctcaaca
    25141 cactgatatt gctgtgttcg ggcaccaccg tcacctgggc gcatcacggg ctgatccatg
    25201 gcgaccgcgg cgcgctgaag aagggcctgt tgctgaccat cgtgctgggc ctgctgttca
    25261 cttcgctgca ggcgtttgaa tatgcccatg cgcccttcgc cttcggcaag aacacctatg
    25321 gttcggcctt ctacatggcc acgggctttc atggcctgca tgtgatcatc ggcacgatct
    25381 tcctcaccgt ctgcctgttc cgcgcctatc ggggcgattt caccccgcgc cagcatttcg
    25441 gcttcgaggc ggcggcctgg tattggcact ttgtcgatgt ggtgtggctg ttccttttcg
    25501 tgtgcatcta tgtgtggggc aattggggat tcccggttga atgagcggtc ggttgaatga
    25561 ccgacttgcc tgataacaac aggttcaggg gggacgggca gccgggcatg atcccggctg
    25621 ccctctttgg cttgtgcccg cgctgcgggg caaaggggct gtttgccggg ctggcgcgct
    25681 ttgccccggc gtgccggcgc tgcgggctcg atttttccgg cttcaacgtg ggcgatgggc
    25741 cggccgcttt cctcaccttt ggcgtggggg cgctggtggt ggtggtggca gcctggctgg
    25801 cgctgtcggt cgatccgccg tggtgggtgt atgtgctggt gctggtgccg ctcaccttcg
    25861 ggcccacgct gtggggcttg cggctgggca aggcggcgtt gctggtggcg gaatatcgcc
    25921 gcaaggccgg cgaggggcgg ctgaaggaat gagcaggcgc gtgccggtga tccccacgct
    25981 ggtcgtgctg ctggcggtgg ccctcatgct gcgcctgggc gtgtggcagc tggaccggct
    26041 gcgcgagaag gaggcgatga tcgcccgtta tcaggccgcg cttgccctgc cggcgctgga
    26101 ttgggcagag ggcgtgccgc tgggcgcggc cccggcctat cgccgggtgc ggctggcctg
    26161 ccagcatttt ggccagccgc gcatggtggg cgggcgcaat gggcggggcg ttgccggctg
    26221 ggccttgtgg gccgattgca cggcgccaag cggcagcatc acggtggtgg cgggctggtc
    26281 tgcccggccc gagacggcag cgctgccggg cgcggccggg gtgagcggca tcgtgctggt
    26341 gggcaagggg ggcgagatgc ggctggtggc cgatccgccg ctggccggcc ttgccgccaa
    26401 tgcccggccc gatccgcacg atctgcccaa caaccacttc tcctatgcga tgcagtggtt
    26461 cgcctttgcc gccacggcgc tggtgatcta tggcctggcc ctgcgcgcgc gctggcggcg
    26521 cggcgccagc gattttggcg gcgcttcagg cagcgcctga ggccccgcaa cccttgcgca
    26581 aagcgcgcat ggcggctaac cggcgcggct gaacaatgca ggactggcac atgcgctata
    26641 tctccactcg cggcaacgca ccggctctcg atttcgaagg cgcgactttg gcgggccttg
    26701 ccagcgatgg cggcctttat gtgcccgaaa gctggccgcg gttctcggcc gcggagattg
    26761 ccgcgctggc gggcctgccc tatgccgagc tggccgcgcg catcatgcag cccttcgtgg
    26821 gcgagagcct gccctatgag cgcctgctgg agctgacccg cgccgcctat ggccgctttg
    26881 cccatgccgc tgtcaccccg ctcaagcagt ttgacgagca gcactggctg ctggaactgt
    26941 tccacgggcc cacgctggcc ttcaaggatg tggcgctgca gctgctgggc ctgttcttcg
    27001 aggaattcct cgcccgcggc agccagaacc tcaccatcgt cggcgccacc agcggcgata
    27061 ccggcagcgc ggccatcgat gcggtggccg gccgcgccaa ggtggatatc ttcatgctgc
    27121 accccaaggg ccgcgtctcc gacgtgcagc gccggcagat gaccaccgtg ctggccccca
    27181 atgtgcacaa catcgccatc accggcacgt tcgacgatgc ccaggcgatg gtgaagcgca
    27241 tgttcaacga tgaggcgatg accggccgct ttgccattgg cgcggtgaac tcgatcaact
    27301 gggcgcggct gatggcgcag gtggtgtatt atttcgccgc tgccctgcag ctgggcgcgc
    27361 cgcatcgcaa ggtggccttt gccgtgccca cgggcaattt cggcgatgtg tttgccggct
    27421 atgtggcagc ccaaatgggc ctgccgatcg agcggctgat cgtggccacc aatgtgaacg
    27481 acattctcca ccgggcgctg gctaccggcg attattccgc cggcacggtg acgcccaccg
    27541 ccgcgcccag catggatatc caggtctcgt cgaatttcga gcggctgctg ttcgatgtcg
    27601 gcgggcgcga tggcgcggcg ctggccgcgc agatggccgg ctttgaggca acgaaggcga
    27661 tgaccctcac caacgcccag cgcgaggggg cagcagcgct gttctccagc gcccgcgccg
    27721 atgccgacga catggcccgc gccatccgct gggcgtggga tgcggccggc gagctgatcg
    27781 atccgcacac cgccattggc ctgcatgccg cgcgcgccgc aggtgtgccg cgcgaagtgc
    27841 cggtggtgac gctggcgacc gcccatccgg ccaagttccc cgaggcggtg gagcggtcga
    27901 ccggccatcg cccggcgctg cccgagcgtg tgggcgacct gttcgagcgc gaggagcgct
    27961 gcgcggacct gcccggcgat tatgccgcga ttgccgatta tgtggccgcc catgccacgc
    28021 cgaaggcctg aatggctgcg ttgagcctcc agcccctgct gctgacgggg gaaggctggg
    28081 cggattacgg gctggtggat tcgggcgcgg gccgcaagct ggaacgctat ggcccgatgc
    28141 gcttcatccg gcccgagccg caggccctgt ggcagccgcg cctggccgat tggcaggcgc
    28201 agggcgaatt cgtgcccggc gccgacgagg atggcggcgg gcgctggcac ttcgaagcgc
    28261 cggtgcccgg ggaaggctgg ccgctggcct gggccaatgg cgaggatgtg gtgcgcttca
    28321 ccgcgcaatg cacgcccttc cgccatctgg gcttcttccc cgatatggcg ccggtgtggg
    28381 gctggatggg cgagcagctg gcggggcgca gcgatgcctc ctgcctcaac ctcttcggct
    28441 acacgggcgt gggcacgctg gcgctcagcc gctttggcgc ggtgacgcat gtggatgcca
    28501 gcaagaaatc ggtagcgcag gcgcgtgcca atgcggcgct ctccggcgcc gagcagcgcc
    28561 cgatccgctg gatcgtcgat gatgccgcca aatttgccgc ccgcgaagtg cgccggggca
    28621 agcgctacga cgggatcatc ctcgatccgc ccaaattcgg ccgcgggccg gaaggcgagg
    28681 tgtggcggct ggaagagcat ctgcccggcc tgatcggcga ttgccgccgc ctgctcgatg
    28741 ccgacagccg cttcctcttc ctcaccgtct atgccgtgcg gatgagctcg ctggcgattg
    28801 ccggcctgct ggccgaagcc ttcgccgatc tgcccggcac tatcgagcat ggcgatctgg
    28861 ccgtgcgtga ggatgcgcgc gaaggggcag agggccgcct gctgcccacg gccatctttg
    28921 cccgctggcg cgccggctga ggcggcgctg ccactggact tgtgcgccgc aaaatgccat
    28981 tgccgccgcc atttgccggc ccgctgctgg cagattgcct caacttattg aaagccttgc
    29041 ataacgtggc cgacagcctg attctcgaag tggccgattt tgaatccgac gtgctgaccg
    29101 gcatctattc ggaggaaacc ggcaaggccc agccgctgcg catcaccgtg gccgtgcggc
    29161 taacgccggc gccgcgctat gcgcccgaca cggcgctgtc ggccagcaag aattacatgg
    29221 acctgaagtt tgccgccagc caggcgctgc ccgaagggct gcacttcacc ctgatcgagg
    29281 cggtggccga ccatatctgc gaaaccctgt tcctgcagga tcagcgggtg gaagcggtga
    29341 cggtgaagat cgtcaagctg gcgctctcgg aaaagggcga gaagattggc gtgacgctga
    29401 cccgcgagcg gcgctgatgg aattgcgccg gatcgtcgat ttgcccgatg atccgctggc
    29461 ggccgccgct gcctttcatg cgctgcatct gccggccctg cgcgcggcca cgggcgatgt
    29521 ggcgatcctg ttcgccccgg cagagcacac ccatgccggc tggcggttgg ccgcggtgca
    29581 ggcgctggcg cgcgcggcca caccgcggcg ggtgaatgcc atagagggcg ccggggaagc
    29641 ggcgattgcc gcggcctgcg cctggctggc ggcggccccg ggcgtgaccg ggcagatcct
    29701 gcggctggct ggcggcgcgg gcgcgggggt gataggctga ggggatgggc gctgcacctc
    29761 ctcttgtcga tggccttcgc cggcgcatca cctatctgcg cctcagcgtg atcgaccgct
    29821 gcgatttgcg ctgcacctat tgcatgcccg agcgccagca gttcctgccg cgcgccgagg
    29881 tgctgagcct ggaagagctg caccggctgg cgctggcctt catcgcccgc ggggtgacca
    29941 agctgcggct gaccg
//
LOCUS       DASRYE010000297         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_187205,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000297 DASRYE010000000
VERSION     DASRYE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187205"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="VFF98_15325"
     CDS             complement(<1..1967)
                     /locus_tag="VFF98_15325"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU65056.1"
                     /translation="MPPRPDGARRGALGILLGAPLLLAGPVARAGSYEVPAGPLGQVL
                     ARIGAVGHATILVTDPALGTRPSRGVHEARSLHGALRQAMRGIDARAQFIDPRTIRVS
                     AMPRRAARAPLAAPAPLASADILVTASKQAVDLDHYPGSAKIIATDRGWLAQNAASGT
                     GAIARLLPILASTNLGDGRDKLFIRGIADSSFNGPTQSTVGEYLGDIRLNYNAPDPHL
                     NLYDMDRVEVLVGPQGTLYGAGTLGGIIRFVPNAPDSHAFAATASAGLSSTWSGAVGE
                     DGAAMVNLPLAHGRAAVRMVAYAAQAGGYIDDPSRGLKNINRSSSYGQRLSVRLDDVG
                     GWRLDLGMVFQNLYSADGQYTLLGTPALTRANAIAQPFHNNERMGYLSARRVLGWADL
                     TGSLGFVKQDLGTVFDATGYDGTATPARYSELNDITLFTGEFRLSGHSRRHPWVLGAS
                     ALYNASALMRSLGPLAEEATLTTVVNTRSEGALFGEVSFPLGRRFTGTLGERLTVANS
                     TGFLVDASGAFTNRLAHVSARLSTTLALDWHPGGPVSAFFHYQQGYRPGGLSVAASGS
                     AVQSQRFETDRLSMDELGLRYGRQEHDRLWLRGALFFADWANIQADLVGSNGLVATAN
                     IGHGFIYGLDGEIGWHPAPAWTLSAAAFVND"
     gene            complement(1951..2928)
                     /locus_tag="VFF98_15330"
     CDS             complement(1951..2928)
                     /locus_tag="VFF98_15330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZU65057.1"
                     /translation="MTETAADLIQTALSWHIRLRDGGDAASDADWAAFADWLAAHPDH
                     AGAYAEVEAMDHALDPALPDLHFPAPAPVAANDDAPPRRWRRAAGVGGGLAAALAVAL
                     FTGWFMEHGSSRYQIATAPGESRTILLDAGTEVTLNGDTQMVFDRKDTRFARLDHGEA
                     LFAVRHDARHPFTLEVSAGRVVDLGTRFDVVSLARQTRIAVAEGAVDYRPDGQSIPLH
                     AGQQMQADAGAGTVQISPVDRADVGGWQHRRLSYDGSPLEQVAQDLGRVLGAAIRVDA
                     AIARQPVFGTIDVQEITPARIPLLASALNVRVVRQGKIWILKPAHAPTP"
     gene            complement(2921..3487)
                     /locus_tag="VFF98_15335"
     CDS             complement(2921..3487)
                     /locus_tag="VFF98_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU65058.1"
                     /translation="MPDSGLLQTFLNARPELLRFLRLRGAMPEEAEDILQNLGLKLAA
                     QATGPVDQPRAYLYRMATNAFLLHRRGDARRRRRDEDWVDAQSGFMREIDPQPSAETA
                     LIASQQLRLLQAVIDAMPERTATIFRRFRLEGVAQKTIAADLGISVSAVEKHLARAYL
                     DIWQARKKSDEETGPVRHPAHEQGHHHD"
BASE COUNT          627 a         1285 c         1298 g          567 t
ORIGIN      
        1 ctgtcgttga cgaaagcggc cgcgctcagc gtccatgccg gggccggatg ccagccgatc
       61 tcgccatcca gcccatagat gaagccatgg ccgatgttgg cggtggcgac aaggccgttg
      121 ctgcccacca gatcggcctg gatattggcc caatcggcaa agaagagggc gccgcgcagc
      181 cacaggcggt catgctcctg gcggccatag cgcaggccca gttcatccat gctcagcctg
      241 tcggtctcga agcgctggct ttgcaccgcg ctgcccgatg ccgccaccga aaggccgccg
      301 gggcgatagc cctgctgata atggaagaag gccgagaccg ggccgcccgg gtgccagtcg
      361 agcgccagcg tggtggacag ccgcgccgag acatgggcca ggcggttggt gaaggcgccg
      421 ctggcatcga ccagaaagcc cgtgctgttg gccaccgtca gccgctcgcc cagcgtgccg
      481 gtgaagcggc ggcccagcgg aaaggacacc tcgccgaaca gggcgccttc gctgcgggtg
      541 ttcaccacgg tggtcagggt ggcttcctcg gccagcggcc ccaggctgcg catcagcgcg
      601 ctggcattgt agagcgccga ggcgcccagc acccagggat ggcgccggct gtgcccgctg
      661 aggcgaaact cgccggtgaa cagggtgatg tcgttcagtt cgctgtagcg ggccggcgtg
      721 gcggtgccat catagccggt ggcatcgaac accgtgccca gatcctgctt cacaaagccc
      781 aggctgccgg taagatcggc ccagcccagc acgcgccggg ccgagagata gcccatccgc
      841 tcattgttgt ggaacggctg ggcgatggcg tttgcccgcg tcagcgcggg cgtgcccagc
      901 agcgtatatt gcccgtcggc gctgtagagg ttctggaaca ccatgcccag atcgagccgc
      961 cagccgccca catcatctag ccgcaccgac aggcgctggc catagctgga actgcggttg
     1021 atgttcttca gcccgcgcga gggatcgtcg atatagccgc ccgcctgggc cgcataggcc
     1081 accatccgca ccgccgcccg gccatgggcc agcggcaggt tgaccatcgc cgcgccatcc
     1141 tcgcccaccg cgccagacca tgtgctgctg agccccgccg aggccgtggc ggcaaaggca
     1201 tggctgtcgg gcgcgttggg cacgaagcgg atgatgccgc ccagcgtgcc cgcgccatag
     1261 agcgtgccct gcggccccac cagcacttcc acccggtcca tgtcgtagag gttgagatgg
     1321 ggatcggggg cgttgtagtt caggcggatg tcgcccagat attcgcccac ggtggattgg
     1381 gtggggccgt tgaagctgct gtcggcgatg ccgcggatga acagcttgtc gcgcccgtcg
     1441 cccagattgg tggaggccag aatgggcagc agccgggcaa tggcccccgt gcccgaggcc
     1501 gcgttctgcg ccagccagcc gcgatccgtg gcgatgatct tggccgagcc gggataatgg
     1561 tcgagatcga ccgcctgctt gctggcggtg acgaggatat cggccgacgc cagcggcgcg
     1621 ggcgcggcaa ggggcgcccg cgccgcgcgg cggggcatgg ccgaaacgcg gatggtgcgc
     1681 ggatcgatga attgcgcccg ggcatcgatg ccccgcatcg cctgacgcag cgcgccatgc
     1741 agcgagcgcg cctcatgcac cccgcgcgag ggcctggtgc ccagcgccgg atcggtgacg
     1801 agaatggtgg catggcccac cgcgccaatc cgcgccagca cctgccccaa agggccagcc
     1861 ggaacctcat agctgcccgc ccttgccaca gggccggcca gaagcagggg cgcgcccagc
     1921 aggatgccca gcgcaccccg ccgcgcgcca tcagggcgtg ggggcatggg cgggcttcag
     1981 gatccagatc ttgccctgcc gcaccacccg cacattgagg gccgaggcaa gcagcgggat
     2041 gcgcgcaggg gtgatctcct gcacatcgat cgtgccgaac accggctggc gggcaatggc
     2101 cgcatccacc cggatcgccg cgcccagcac ccggcccaga tcctgcgcca cctgctccag
     2161 cgggctgccg tcatagctca gccggcggtg ctgccagccg cccacatccg cgcggtccac
     2221 aggcgagatc tgaaccgtgc ccgccccggc atcggcctgc atctgctgcc cggcgtgcag
     2281 ggggatggat tgcccgtcgg ggcggtaatc gacagccccc tcggccacgg cgatgcgcgt
     2341 ctgccgggcc aggctgacca catcgaaccg cgtgccaaga tcaaccaccc tgcccgcgct
     2401 cacctccagc gtgaagggat ggcgcgcatc atgccgcacg gcaaacagcg cctcgccatg
     2461 atcgagccga gcgaagcggg tgtccttgcg gtcgaacacc atctgcgtgt cgccgttgag
     2521 cgtgacctcg gtgcccgcat cgagcaggat cgtccggctt tcacccgggg cggtcgcgat
     2581 ctgatagcgg ctggagccgt gttccatgaa ccagcccgtg aacagcgcca cggccagcgc
     2641 cgccgccaac ccgccgccca cgcccgccgc ccggcgccag cggcgcgggg gggcatcgtc
     2701 attggccgca accggcgccg gcgcggggaa atgcagatcg ggcaaggcag gatcaagcgc
     2761 gtgatccatc gcttccacct cggcataggc tccggcatgg tcggggtgcg cggcaagcca
     2821 gtcggcaaag gcggcccaat cggcatcgct ggccgcatcg ccgccatcac gcaggcggat
     2881 gtgccagctc agcgcggttt ggatcaggtc ggcggcggtc tcagtcatgg tgatggccct
     2941 gttcatgagc aggatgccgc acaggcccgg tttcctcatc actttttttg cgggcctgcc
     3001 aaatgtcgag ataggcccgc gccagatgct tctccaccgc gctgacgctg atgccaagat
     3061 cggcggcgat ggtcttttgc gccacgcctt ccagccggaa ccggcggaag atggtggcgg
     3121 tgcgctctgg catggcgtcg atcacggcct gcagcaggcg cagctgctgg ctggcgatca
     3181 gcgcggtttc ggccgagggc tgcgggtcga tctcgcgcat gaagccgctt tgcgcatcca
     3241 cccagtcctc gtcgcgccgc cgccgccggg catcgccccg gcggtgcagc aggaaggcgt
     3301 tcgtggccat gcgatagaga taggcgcgcg gctgatcgac cggccctgtc gcttgcgccg
     3361 ccagtttcag gcccaggttc tggaggatgt cctcggcctc ctcgggcatg gcgccgcgca
     3421 gccgcaggaa gcgcagcagc tcgggccggg cgttgaggaa ggtctgcagc agaccgctat
     3481 cgggcatgga gcatctgtgg cgtttcgagg aaggggagca aggcttcgtg cacgcgtgag
     3541 gataaaaccg ttggggcccg cgtcaacagg cgggaaaacc gccgggatca taattaaaac
     3601 aaatcatttt cagatattta tgaaaatttt acaggccaac gccaaattcc gctgaggctg
     3661 gccggaagcc acggtgtctc agccatgaaa ggacgagacc gatgcccccc gacgatgccg
     3721 aatgcccccc tgagccttat cttgcgccct cgctggcgcc gctggagcgg acggtgg
//
LOCUS       DASRYE010000298         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_169489,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000298 DASRYE010000000
VERSION     DASRYE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169489"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFF98_15340"
     CDS             complement(<1..299)
                     /locus_tag="VFF98_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZU65059.1"
                     /translation="MSDSLSVERERSGRFLPRFDDRGLLTGIAVDSRSGEILMVAFMN
                     AEALAATRATGFAHFYSRSRQAQWMKGETSGHVLKVVEMRVDCDQDAIVMRVEPAG"
     gene            complement(704..1492)
                     /locus_tag="VFF98_15345"
     CDS             complement(704..1492)
                     /locus_tag="VFF98_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZU65060.1"
                     /translation="MSLADALAPLVDAVFPPRCPLCGGGIAQHGDLCARCWHGLAFPT
                     GAACGHCGQPLAPEEACLPCAGAAGAAVGAADSICAATRYNPAARQLALALKRGGKLA
                     LAPLMVRLMLARLAAEGRMPGPGWLVVPVPLHRWRLWRRGFNQSALLARGIARASGAR
                     LVVDGLQRVRATRSLGDLGAEERRAELAGAIAASRRRTGLLHGARVLLVDDVVTSGAT
                     SGACVAALRAAAARQVHIACFARVATPAPRPGGRAEALEPEPDA"
     gene            1567..2340
                     /locus_tag="VFF98_15350"
     CDS             1567..2340
                     /locus_tag="VFF98_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZU65061.1"
                     /translation="MTTPPPPRIFAPQRRLAARRRMARLQGLPNAPRYLLDDMVEDVL
                     DRLSFLRHAPQRALVVGDVHGDLAAALAAQGAEVTRADPAALGGAMALEEERPYPLSG
                     FDLIASLATLDTLNDLPGALIHIRQALAPGGLMIASFMGAGSLPALRAIALAADGERP
                     APRLHPMVDVRAGGQLLQRAGLANPVVDARGLDVRFGSLSALVADLRGQALGGLLAQG
                     GPAWGKAARARAEAAFAEAADHGRLTEHFEILTLSGWRI"
     gene            complement(2427..>4217)
                     /gene="ppdK"
                     /locus_tag="VFF98_15355"
     CDS             complement(2427..>4217)
                     /gene="ppdK"
                     /locus_tag="VFF98_15355"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZU65062.1"
                     /translation="TPQYLTKRAREAAGAKPLSMEEAMPEAYGELARVFDLLENHYRD
                     MQDIEFTVERTKLWMLQTRSGKRTAKAALKMAVDMVEEGLIDEATAIRRVDPMALDQL
                     LHPTLDPDAPRDHLGKGLPASPGAASGAIVLDAETAQTLAERGDAVILVRVETSPEDI
                     HGMHAAKGILTARGGMTSHAAVVARGMGRPCVSGASSLSIDMGARTLKIGNRDLKEGD
                     VITLDGATGDIMAGSVPTIEPELVGDFATLMSWADRHRRMGVRTNAETPEDCRMARSF
                     GAEGIGLCRTEHMFFDANRISAVRQMILAEDEAGRRAALAKLLPEQRADFHAIFEIMT
                     GLPVTIRLLDPPLHEFLPHGDAEFEELSEAIGIGVATLKRRADELHEFNPMLGHRGCR
                     LGITFPEIYEMQARAIFEAACDVAAASGGAVVPEVMIPLVATKRELAILKALVDKTAA
                     EVFAEKGRVLDYTVGTMIELPRAALMAGEIAEEGKFFSFGTNDLTQTTLGVSRDDAAR
                     FLSAYVEQGIYARDPFVSLDVDGVGQLVTLAAERGRATRPDIKLGICGEHGGDPASIA
                     FCEKVGLDYVSASPYRVPIARLAAAQAALG"
BASE COUNT          642 a         1507 c         1440 g          628 t
ORIGIN      
        1 cccgccggct ccacccgcat cacgatcgcg tcctgatcgc aatccacgcg catctccacc
       61 accttcagca catggcccga ggtttcgccc ttcatccact gcgcctggcg cgagcgggag
      121 tagaaatggg caaagccggt agcgcgggtg gccgccagcg cctcggcgtt catgaaggcc
      181 accatcagga tctctcccga gcgcgaatca acggcaattc cggttaacag gcccctgtcg
      241 tcgaagcggg ggaggaaccg gcccgaacgc tcgcgctcga ccgaaagact atccgacatg
      301 aacaattttc cttggctggg aaccctgggg cccgtcactt gttgcacgat aaccacagag
      361 ccgggccaaa atccacaggc tctgacataa gatctttatc ttcccgtcat tccggtgaaa
      421 caaatggcca tgcggttttg agggaagccg ctgtcggtag gcagcatgat gcgagaacgg
      481 aactttccgc cgcatccagg caaacagcgc cacggttcag ggaatggtcg agcccaaacg
      541 ggcgggccaa caggtttgag ggtttgggcg ggacagccgg gtaaaccggg agtcacaaag
      601 gttaataacc agccttgtca gtttcgtcac gaggaagcgc ccggagctta ggctccgggc
      661 gcttctttct tgtcggctgc tgcaggtttt gtgcgcagca tcgctaggcg tcgggctctg
      721 gctccagcgc ttctgcccgc ccgcccgggc gcggcgccgg cgtggccacc cgggcaaagc
      781 aggcgatatg cacctgccgc gccgccgccg cccgcagcgc cgccacacaa gcgcccgagg
      841 tggccccgct ggtcaccaca tcatccacca gcagcacgcg ggcgccgtgc agcaggccgg
      901 tgcgccggcg gctggcggca atcgcgccgg ccagttccgc gcgccgttcc tccgccccca
      961 gatcgcccag cgagcgcgtg gcgcgcaccc gctgcaagcc atccaccacc agccgcgcgc
     1021 cactggcccg ggcaatgccg cgcgccagca gggcagactg gttgaacccg cgccgccaca
     1081 gccgccagcg atgcagcggc accggcacca ccagccagcc cggccccggc atcctgccct
     1141 ccgccgccag ccgcgccagc atcagccgca ccatcagcgg ggccagcgcc agcttgccgc
     1201 cgcgcttcag cgccagcgcc agttggcggg cggcggggtt gtagcgcgtg gcggcgcaaa
     1261 tgctgtctgc cgcgccgacg gcagcgcccg cggccccggc gcagggcagg caggcctcct
     1321 cgggcgccag cggctggccg caatggccgc aggccgcgcc cgtgggaaaa gccagcccat
     1381 gccagcagcg cgcgcacaga tcgccatgct gcgcaatgcc gccgccacac agggggcagc
     1441 gcggcggaaa caccgcatcg accaacggcg caagggcatc ggcaaggctc acggcgcgca
     1501 gtctaaccgc tggggcggcg ccggcaaggc ttgcgcgcgc cggcaagcag gggcaggagc
     1561 aggcgcatga cgacgccccc cccgccccgc atcttcgccc cccaacgccg cctggccgcg
     1621 cggcgccgca tggcccgcct gcagggcctg cccaatgctc cgcgctatct gctggacgat
     1681 atggtggagg atgtgctcga ccgcctgtcg ttcctgcgcc atgcgccgca gcgggcgctg
     1741 gtggtgggcg atgtgcatgg cgatctggcc gcggcgctgg ccgcacaggg cgcggaggtg
     1801 acgcgggcag accctgccgc cctcggcggc gccatggcgc tggaggaaga gcggccctat
     1861 cccctctctg gcttcgatct catcgccagc ctggccacgc tcgacacgct gaacgacctg
     1921 cccggcgcgc tgatccatat ccgccaggcg ctggcccccg gcgggctgat gatcgccagc
     1981 ttcatgggcg cgggcagcct gccggccctg cgcgccatcg cccttgccgc cgatggcgag
     2041 cgccccgcgc cgcgcctgca cccgatggtg gacgtgcgcg ccggcgggca attgctgcag
     2101 cgcgctggcc ttgccaatcc ggtggtggat gcgcgcgggc tggatgtgcg cttcggcagc
     2161 ctgtcggcgc tggtggcgga tctgcgcggg caggcgctgg gcgggctgtt ggcgcagggt
     2221 ggcccggcat ggggcaaggc ggcacgcgcc cgggccgagg cagcctttgc cgaggccgcc
     2281 gaccatggcc ggctgaccga gcatttcgaa attctcacgc tgagcggctg gcgcatataa
     2341 aagggcgcgc gaagggttcc cccctcgcgc gccgttttta tcggccggac ggcgcgaggg
     2401 gttgccccct cgctcttccc gtgggttcag cccagcgcgg cctgcgctgc ggccaggcgg
     2461 gcaatcggca cgcgataggg cgaggccgac acgtaatcca ggcccacctt ctcgcagaag
     2521 gcgatggagg ccggatcgcc gccatgctcg ccgcagatgc ccagcttgat gtccgggcgg
     2581 gtggcacggc cgcgctcggc ggccagcgtc accagctggc ccacgccatc cacatcgagg
     2641 ctgacgaagg gatcgcgcgc atagatcccc tgctcgacat aggccgagag gaagcgcgcg
     2701 gcatcgtcgc ggctgacgcc cagcgtggtc tgggtgagat cgttggtgcc gaaggagaag
     2761 aacttgcctt cctcggcaat ctcgcccgcc atcagcgccg cgcgcggcag ctcgatcatc
     2821 gtgcccacgg tgtagtccag cacgcggccc ttctcggcga agacctcggc ggcggtcttg
     2881 tcgaccagcg ccttcaggat cgccagctcg cgcttggtgg ccaccagcgg gatcatcacc
     2941 tcgggcacca ccgcgccgcc gctggccgca gccacgtcgc aggcggcctc gaagatggcg
     3001 cgggcctgca tctcgtagat ttccgggaag gtgataccca ggcgacaacc gcgatggccc
     3061 agcatcgggt tgaactcgtg cagctcatcg gcccggcgct tcagcgtggc cacgccaatg
     3121 ccgatcgctt ccgacagctc ctcgaattcc gcatcgccat gcggcaggaa ttcgtgcagc
     3181 ggcggatcga gcaggcggat ggtgaccggc aggcccgtca tgatctcgaa gatggcgtgg
     3241 aaatcggcgc gctgttccgg cagcagcttg gccagcgccg cgcggcggcc ggcctcgtcc
     3301 tcggcgagga tcatctggcg cacggccgag atgcggttcg catcgaagaa catgtgctcg
     3361 gtacggcaca ggccaatgcc ctcggcgccg aagctgcgcg ccatgcggca atcttccggc
     3421 gtttcggcat tggtgcgcac gcccatgcgg cggtgcctgt cggcccagct catcagcgtg
     3481 gcgaaatcgc ccaccagttc gggctcgatg gtcggcacgc tgccggccat gatgtcgcca
     3541 gtggcgccat caagggtgat gacatcgcct tccttcaggt cgcggttgcc gatcttcagc
     3601 gtgcgcgcgc ccatgtcgat gctgagcgag ctggcgcccg agacgcaagg ccggcccatg
     3661 ccgcgggcca ccacggccgc gtggctggtc atgccgccgc gcgcggtgag gatgcccttg
     3721 gccgcatgca tgccgtggat atcctcgggc gaggtttcca cgcgcaccag gatcaccgca
     3781 tcgccgcgct cggccagggt ctgggcggtt tcggcgtcga gcacgatggc gcccgaggcc
     3841 gcgccgggcg aggccggcag gcccttgccc agatggtcac gcggggcatc gggatcgagc
     3901 gtggggtgca gcagctggtc gagcgccatc ggatccaccc ggcggatcgc cgtcgcctcg
     3961 tcgatcaggc cctcttccac catgtccacc gccatcttca gcgcggcctt ggcggtgcgc
     4021 ttgcccgaac gggtctgcag catccacagc ttggtgcgct cgacggtgaa ctcgatgtcc
     4081 tgcatgtcgc ggtagtggtt ttcgagcagg tcgaacacgc gggccagctc gccataggcc
     4141 tccggcatcg cctcttccat cgacagcggc ttggcccctg ccgcctcgcg ggcgcgcttg
     4201 gtgaggtatt gcggcgt
//
LOCUS       DASRYE010000299         9437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_64813,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000299 DASRYE010000000
VERSION     DASRYE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64813"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFF98_15360"
     CDS             <1..118
                     /locus_tag="VFF98_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZU65063.1"
                     /translation="LHCSRIDGSPLRYNQPNTYMPDLLICRPEHAQHILSLI"
     gene            complement(143..772)
                     /gene="folE"
                     /locus_tag="VFF98_15365"
     CDS             complement(143..772)
                     /gene="folE"
                     /locus_tag="VFF98_15365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZU65064.1"
                     /translation="MPETRGPEARGPEARGPEASPPALPAEVLAAVATLIRWSGDDPA
                     REGLRDTPARVARAWREYCEGYREDPARHLARVFHEVGGYDEIVLLKDIPFQSHCEHH
                     LAPILGRAHIAYLPGDHVVGISKLARVLHGYARRLQVQERLTAEVADCIWQHLRPRGV
                     AVVIEATHGCMTGRGVRTHGVTMKTSRMMGIFREDEKSRREVLALMERG"
     gene            complement(781..2013)
                     /locus_tag="VFF98_15370"
     CDS             complement(781..2013)
                     /locus_tag="VFF98_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU65065.1"
                     /translation="MSTTAEAGPRAAAPLYRGPIFDADTHFWETEEAWTKYLPKEAAE
                     KYGVSFRRGDDGDFAMYVGPRKVEISADHLFEDGRVPAPGKLHEWLRAMKEGKAEIDL
                     HVPKSADMMEPAARVALLDSWDVRSSILFVGNMITAISFLDDPRYAYDVLNAYNGWMY
                     DQWTYNYQDRIFSAPIVTLDNLEEACKQLKGVIARGARLILMPMGPANRKAPAHPDHD
                     PFWQIANEAGIRVVFHVSEAIYMKEHMALWGEGMQQSRIRQSAFVWMHGYSERPVIET
                     LSSFIFWNFFERFPNVKLLSAENGAEWVPAMLTKMDKCRGIAKNGHWPAGQLKERPSR
                     IFARHVSVVAYPEDNIAALVDQVGNADWVLMGSDYPHAEGVEEPRQFADEACRGLSEA
                     DMRKVMYENGMRFMGLEA"
     gene            complement(2157..2894)
                     /locus_tag="VFF98_15375"
     CDS             complement(2157..2894)
                     /locus_tag="VFF98_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU65066.1"
                     /translation="MTDRFDTIALTRAGGITELRIHHQGESARWALWGGLHRELGEAF
                     AAIAADRDTRALIITGTGSSFCAEFDMTSQPPPMGPQSWDVIFREGRALLENLLAIEV
                     PVIAAVNGPAFIHAEIPLLADIVLAAPGAVFADKAHALAGVVPGDGVHVVWPMLLGPN
                     RGRYFLMTGQEIGAAEALALGLVGEVVAGDALLERAREHARAIAARPALAMRYARIAL
                     TRHLRERLKAELGYGLMLEGMAVLGGA"
     gene            complement(2922..4094)
                     /locus_tag="VFF98_15380"
     CDS             complement(2922..4094)
                     /locus_tag="VFF98_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HZU65067.1"
                     /translation="MRQWDEFVDLLKPAEALLDQLRAPRSEQLRAELYRQFAMNLAQG
                     YLLYFQTSAEYPEFMPFENSAFLAQPNPDAVYYYTRVDGAGSYRVVGERGDAIVAGFA
                     VGNRMIGMAGSPGQGLANYDIDQLEIGADGRFEVLFSAERPAGHVGNWRYLHPEADFL
                     LVRQFNYDWGGDRDMRLAIERLDPLPPKPRLSPERTDALLGELFGTYARGLSAIALGA
                     LGRAFDKGLVNKVALHDFKDLGNSADWPQAYWEMVFELAEDEALVIESDLPEARHYWN
                     VQVIDGLWNQADILYRQTSLNGRTARIDADGRFRAVLCRQDPGYANWLDTADHGFGML
                     IGRWYRCSSHPVPQVRRMKLAEVATYLGDRSARISPEARRAALRERLIGAQWRRRW"
     gene            complement(4138..5139)
                     /locus_tag="VFF98_15385"
     CDS             complement(4138..5139)
                     /locus_tag="VFF98_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU65068.1"
                     /translation="MRAAVFKGAGQPWAIESLPDPQPGAGEVVIKVCRCGLCGTDLHM
                     TSGSGWDFPAETVPGHEYAGEVVALGAGVEHLAIGDRITALPAKGCGQCAACRAGQVM
                     LCPQMQGYMGGFGEYLRAPAAHAVKLPATLSFADGALIEPLAVGLHGVAMAPLVPGSR
                     VLVLGAGSVALAAIYWARRLGAGRIAVMSRSARRADLAGQLGGDAFIPYGEGEHGEVI
                     AALGGQPDIVLECAGAVGMLGKAVEHARPNGTIVSMGFCTHPDPVIPGIATYKQLRLV
                     FSMAYTLAEFEHVARTLDRGHLEPRLMVSETIPLDALPAMIEGLRAGASQTKVQVAL"
     gene            5578..7995
                     /locus_tag="VFF98_15390"
     CDS             5578..7995
                     /locus_tag="VFF98_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65069.1"
                     /translation="MKNLHIARLAMTLAASTALNTLAPALAHAADAAADEAPPAPAPA
                     AREGIADIIVTARKTSEKMQDVPVAVTALSGAKLQQQSIMSVQDVQFHVPGFVEYPEA
                     QGGAPDFAIRGAKQQGVSGSQGGVAVYLDYIPLSSNYSIATATYDMQAVQVLKGPQGT
                     LFGKNTTGGAIIFSPNKPTDKFEGDFKAEYGNYNRVDLTGMLNLPITEGVALRVAGRY
                     VHRDAYITNVGASGSLGNFDSENNQSLRATLRIQPNEAITSDTMFDYYHQKQNPNHDR
                     LVGYNAAFICSIYPNTCSGGKSTPYERELSLPLREVDLGDQQNINHGTYWGIANTTTV
                     QLGTHTSLRNIISYRRDKLDSNETSDGVSIPLLNGLNNNRQRQFTEELDLMGKLFDDK
                     LSYTVGIYYYDDQYDQRSDYQIMAPNTNTVDSSLSYLGEFAEGGGGLPQFPISAYGYS
                     PISPSLQINDFGMKTIAVFGQATYKVDDKLSVTLGGRYNHDKGTFTSEQYQGYTDATL
                     TTPLCNVLAYADASELVGACKIARSKLWQSFTWNASLNYKADPRTLLYVSAGRGYQAG
                     GFNQQIRESQYRTFSPEVVFTLEAGLKKDWHLGGRPIRTNIAIFRSNYKNQQRVENGT
                     YSDGLNFIATFNAASSTMWGGEAEISYLPTPQLEISLAYSYINAKYDYFKSPDVANLG
                     GQDLSSLAIGATPKHTFNTTVSYWVPTNNRDGKLKGSVTAYARSGAYYNDLAQSALTY
                     QASYAIFNARLDLIKTLPFDVGVWVNNLADKNYSLFKFDNLNSLGYASVFLAPPRTFG
                     VDASIHF"
     gene            8192..9334
                     /locus_tag="VFF98_15395"
     CDS             8192..9334
                     /locus_tag="VFF98_15395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZU65070.1"
                     /translation="MATQGKVNEAVQHLLDRLVAEGSEIGVQVCAWLGEEQIVDCWAG
                     LADPATGRKVDGDTLFNVYSVSKAVTAVALHIQAERGLVDYDAPVARYWPEFAQAGKG
                     DVTVRHVLSHVSGVLRMPPEVTPELMCDWDWMCRRIAEMAPAYPAGSRSSYQSMTFGW
                     LVGEVVRRTDPQQRPFGRFIREEIADPLGATDLWMGIPDAAEPRVAKLDDVAVYVAPD
                     NNMMREAQPLAVDLMPDPFERPAVRRACIPAVGGIFNARSQARFWAMLANGGRLGGVR
                     LLSAERVASFAAPRPHFDDADPVFFGMQVPIGWAGFWLGGAEAPPVSAPRNMRALCHP
                     GMGGSIGWADPDLGLAVAFCHNRMFDTIDIAEDNRTRIGDAIREAL"
     gene            9337..>9437
                     /locus_tag="VFF98_15400"
     CDS             9337..>9437
                     /locus_tag="VFF98_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZU65071.1"
                     /translation="MTASWDYIIVGGGSAGCVLAERLSRHPACKVLLL"
BASE COUNT         1649 a         3350 c         2906 g         1532 t
ORIGIN      
        1 cctccactgc agccgcatcg atggctcccc cctccgctac aaccagccca acacctacat
       61 gccagacctc ctcatctgca ggcccgaaca cgcacaacac atcctcagcc tcatctgacc
      121 gctggcgctg aggaaaaggg ccctagcccc gctccatcag cgccagcact tcgcggcggc
      181 ttttctcatc ctcgcggaag atgcccatca tccggctggt tttcatcgtc acgccatggg
      241 tgcgcacgcc gcgcccggtc atgcagccgt gggtggcctc gatcaccacc gccacgccgc
      301 gcgggcgcag gtgctgccag atgcaatcgg ccacttcggc ggtcagcctt tcctgcacct
      361 gcaggcgccg ggcatagccg tgcagcaccc gggccagctt gctgatgccc accacatgat
      421 cgcccggcag ataggcgata tgcgcccgcc cgaggatggg cgccagatgg tgctcgcaat
      481 gcgactggaa ggggatatcc ttcagcagca cgatctcgtc atagccgccc acctcgtgga
      541 acacgcgcgc caggtggcgg gcgggatcct cgcgatagcc ctcgcaatat tcccgccagg
      601 cgcgggccac gcgggcgggc gtgtcgcgca ggccctcgcg ggccggatca tcgcccgacc
      661 agcggataag cgtggccacc gccgccagca cctctgcggg aagggctggc gggctggctt
      721 ccggccccct ggcttccggc cccctggctt ccggccccct ggtttctggc atcgcccgcc
      781 tcaggcctcc agccccatga agcgcatgcc gttttcatac atgaccttgc gcatatccgc
      841 ttccgacagg ccccggcagg cctcgtcggc gaactggcgc ggctcttcca cgccctcggc
      901 atggggatag tcagatccca tcagcaccca gtcggcattg cccacctgat cgaccagcgc
      961 ggcgatattg tcctcaggat aggccaccac cgagacatgg cgggcaaaga tgcggctggg
     1021 ccgctccttc agctggccgg cgggccagtg gccgttcttg gcgatgccgc ggcacttgtc
     1081 catcttggtc agcatggccg gcacccattc ggcgccattc tcggccgaca gcaacttcac
     1141 attggggaag cgctcgaaga agttccagaa gatgaagctg gacagcgtct cgatcaccgg
     1201 ccgctcggaa tagccatgca tccacacgaa ggccgactgg cggatgcgcg attgctgcat
     1261 cccctcgccc cacagcgcca tgtgttcctt catgtagatc gcttccgaca catggaagac
     1321 aacgcggatt cccgcctcat tggcgatctg ccagaaggga tcgtggtcgg ggtgggcggg
     1381 cgccttgcgg ttggccgggc ccatcggcat caggatcagc cgggcgccgc gggcgatcac
     1441 gcccttcagc tgcttgcagg cctcctccag attgtcgagc gtgacgatcg gcgcggagaa
     1501 gatgcggtcc tgataattgt acgtccactg gtcgtacatc cagccattat aggcgttcag
     1561 cacgtcatag gcatagcgcg ggtcgtcgag gaagctgatc gcggtgatca tgttgccgac
     1621 gaacaggatc gaggaacgca catcccagct gtcgagcagg gccacgcgcg cggccggctc
     1681 catcatgtcg gcgctcttgg gcacgtgcag gtcgatctcg gccttgccct ccttcatcgc
     1741 ccgcagccat tcgtgcagct tgccgggcgc cggcacgcgg ccatcctcga acaggtggtc
     1801 ggcgctgatc tccaccttgc gggggcccac atacatggca aaatcgccat catcgccccg
     1861 ccggaagctg acgccatatt tctcggcggc ttccttgggc agatatttcg tccaggcctc
     1921 ctcggtttcc cagaaatggg tgtcggcatc gaagatcggc ccgcgataga gcggcgctgc
     1981 ggcgcgaggg cccgcctcgg cagtggtcga catgggggaa gctctccatc catcgcttgt
     2041 gcagggggcc ggcggcagga acaggcgcgc cggctgttgc cccggttctt acgctgcccc
     2101 tccgggcgct gaaacatgcg cccccgcctc atcagaaccg cgatgtttcg cccccctcag
     2161 gccccgccca gcaccgccat cccctccagc atcaggccat agcccagctc cgccttcagc
     2221 cgctcgcgca ggtgccgggt cagcgcgatg cgggcatagc gcatggccag cgccggcctt
     2281 gcggcaatcg cccgggcatg ttcgcgcgcc cgctccagca gcgcatcgcc cgccaccacc
     2341 tcgcccacca gccccagcgc cagcgcttct gcagcgccaa tctcctgccc cgtcatcagg
     2401 aaatagcggc cgcgattggg gcccagcagc atgggccaca ccacatgcac gccatcgccc
     2461 ggcaccacgc ctgccagcgc atgggccttg tcggcaaaga cagcgccggg ggccgccagc
     2521 acgatatcgg ccagcagcgg gatctcggca tggatgaagg ccggcccgtt caccgccgcg
     2581 atcaccggca cctcgatcgc cagcagattt tcaagcagcg cccgcccctc gcggaagatc
     2641 acatcccagc tttgcgggcc catcggcggg ggctggctcg tcatgtcgaa ttcggcgcag
     2701 aagctgctgc cggtgccggt gatgatcagc gcgcgcgtgt cgcggtcggc ggcgatggcg
     2761 gcaaaggcct cgcccagctc gcggtgaagc ccgccccaca gcgcccagcg ggcactctcg
     2821 ccctggtggt ggatgcgcag ttcggtgatc ccgccggccc gggtaagggc gatagtatcg
     2881 aagcggtcag tcatcttgca gtcctttggg ttgggggcgg atcaccagcg gcggcgccat
     2941 tgcgcgccga tcagcctttc gcgcagggcc gcgcgccgcg cctcggggct gatgcgcgcg
     3001 ctgcgatcgc ccagataggt ggccacttcg gccagcttca tgcggcgcac ctgcggcacc
     3061 ggatgggaac tgcagcgata ccagcggccg atcagcatgc caaagccgtg gtctgccgta
     3121 tccagccagt tggcatagcc cggatcctgc cggcacagca cggcgcgaaa gcgcccgtcg
     3181 gcatcgatgc gggcggtgcg gccattgagg ctggtctggc ggtagaggat gtcggcctgg
     3241 ttccacagcc cgtcgatcac ctgcacgttc cagtagtggc gcgcctcggg caggtcgctc
     3301 tcgatcacca gcgcttcgtc ctcggccagc tcgaacacca tttcccaata ggcctggggc
     3361 caatccgcgc tgttgcccag atccttgaaa tcatgcagcg ccaccttgtt caccagcccc
     3421 ttgtcaaagg cccgccccag cgcccccagc gcaatcgccg aaaggccgcg ggcataggtg
     3481 ccgaacaatt cgcccagcag cgcgtccgtc cgttcgggcg agaggcgcgg cttgggcggc
     3541 agcggatcca gccgctcgat ggccaggcgc atgtccctgt cgccacccca gtcgtagttg
     3601 aactggcgca ccagcaggaa atcggcctcg gggtgcagat aacgccagtt gcccacatgg
     3661 ccggccggcc gctcggcgct gaacagcacc tcgaagcggc catcggcgcc gatctccagc
     3721 tggtcgatat cgtaattggc cagcccctgg ccgggcgatc cggccatgcc gatcatgcgg
     3781 ttgcccacgg caaagcccgc aacgatggca tcgccccgct cgcccaccac gcgatagctg
     3841 cccgcgccat ccacccgcgt gtagtaatag accgcatccg ggttgggctg ggccaggaag
     3901 gccgaattct cgaagggcat gaattcggga tattcggcgc tggtctggaa atagagcaga
     3961 tagccctgcg ccaggttcat ggcgaactgg cgatagagtt cggcgcgcag ctgctcgctg
     4021 cgcggggccc gcagctgatc cagcagggcc tcggccggct tcagcagatc gacaaattca
     4081 tcccattggc gcatcggcct tgctcctgtc atcgcatgga aaaagggcgc ccgccgctca
     4141 aagcgcgacc tgcaccttgg tctggctggc gccggcgcgc aggccctcga tcatcgccgg
     4201 cagggcatcg agggggatcg tctcgctcac catcagccgg ggttccagat ggcccctgtc
     4261 gagcgtgcgg gcgacatgtt cgaattcggc cagcgtatag gccatggaaa acaccagccg
     4321 cagctgcttg taggtggcaa tgcccgggat caccggatcg ggatgggtgc aaaaccccat
     4381 cgagacgatc gtgccattgg gccgggcatg ttccaccgcc ttgcccagca tgcccaccgc
     4441 gcccgcgcat tccagcacga tatcgggctg gccgcccagc gccgcgatca cctcgccatg
     4501 ctcgccctcg ccatagggga tgaaggcatc gccgcccagc tggccggcca gatccgcccg
     4561 ccgcgccgaa cgcgacatca ccgcgatgcg ccccgccccc agccggcgcg cccagtagat
     4621 cgccgccagc gccaccgatc cggcccccag caccagcacc cggctgcccg gcaccagcgg
     4681 cgccatcgcc acgccatgca ggcccaccgc caaaggctcg atcagcgcgc catcggcaaa
     4741 gctgagcgtg gccggcagct tcaccgcatg ggccgccggc gcccgcagat attcgccaaa
     4801 gccgcccata tagccctgca tctgcgggca cagcatcacc tgcccggccc ggcaggcggc
     4861 gcactggccg cagcccttgg ccggcagcgc ggtgatcctg tcgccgatgg ccagatgttc
     4921 cacccccgcg cccagcgcca ccacttcgcc ggcatattca tggccgggca ccgtttctgc
     4981 cggaaaatcc cagccagagc ccgaggtcat gtgcagatcc gtgccgcaca ggccgcagcg
     5041 gcacaccttg atcaccacct cgcccgcgcc cggctgcgga tcgggcaggc tctcgatcgc
     5101 ccagggctgg cccgccccct tgaacacggc tgcacgcatc ttgctctccc acctgcccgg
     5161 ccttgcggca gctgatgcgg ccggccgggt gctgctgtca tggcagcatg ggaagggcgc
     5221 gctgctcccg ttcgggaaca agacgatgcc cctgggcaat gccccctgcc cgaaagcccg
     5281 gccccggccc tgcgccaggg ggcgatcctg ccattttgcg cccccttgcg accagcgccc
     5341 ggccgccccg ggccgatcat aacgcaattg tgatgctttg gccgcgcagg cccgcatggc
     5401 cttgagaagg cggcggcaaa gtccttattc atgcctcacg ccgctgatcg gccatcgcca
     5461 atgggcgagc tgccctcagc cggccactcc actatcgtgg cgggccacct gtgcgcccgg
     5521 ccgctcaaca ctttcgggac gaacccggaa ctatatataa cacccaggga ggggaccttg
     5581 aaaaacctgc atatcgcacg ccttgccatg accctggccg ccagcacggc gctgaacacg
     5641 ctggccccgg cgctggccca tgcggccgat gccgcggccg atgaagcgcc gccggcgccg
     5701 gcgccggccg cccgcgaagg catcgccgac atcatcgtga ccgcccgcaa gacctcggaa
     5761 aagatgcagg acgtgccggt ggccgtgacc gccctttccg gcgccaagct gcaacagcag
     5821 agcatcatga gcgtgcagga cgtgcagttc catgtgcccg gcttcgtcga atatcccgag
     5881 gcccagggcg gcgcgcccga ttttgccatc cgcggcgcca agcagcaggg cgtgtcgggc
     5941 tcgcaaggcg gcgttgccgt ctatctcgat tatatcccgc tctcgtccaa ctattccatc
     6001 gccaccgcca cctacgacat gcaggccgtg caggtgctga agggcccgca aggcacgctg
     6061 ttcggcaaga acaccaccgg cggcgcgatc atcttctcgc ccaacaagcc gaccgacaag
     6121 ttcgagggcg atttcaaggc cgaatacggc aattacaacc gcgtggacct gaccggcatg
     6181 ctgaacctgc cgatcaccga gggcgtggca ctgcgcgtgg cgggccgcta tgtgcaccgc
     6241 gatgcctata tcacgaatgt cggcgccagc ggcagcctgg gcaatttcga cagcgagaac
     6301 aaccagtcgc tgcgcgccac gctgcgcatc cagcccaacg aagcgatcac cagcgacacg
     6361 atgttcgact attaccacca gaagcagaac cccaaccacg accgcctcgt gggctataat
     6421 gcggccttca tctgctcgat ctatcccaac acctgcagcg gcggcaaaag caccccttac
     6481 gagcgcgagc tgagcctgcc cctgcgcgag gtggatctgg gcgaccagca gaacatcaac
     6541 catggcacct attggggcat tgccaacacc accaccgtgc agctgggcac ccacacctcg
     6601 ctgcgcaaca tcatcagcta ccggcgcgac aagctggaca gcaacgagac cagcgacggt
     6661 gtctcgatcc cgctgctcaa tggcctcaac aacaaccgcc agcgccagtt caccgaagag
     6721 ctggacctga tgggcaagct gttcgacgac aagctgagct atacggtcgg catctactac
     6781 tacgacgacc agtacgacca gcgctccgac tatcagatca tggcgcccaa caccaacacg
     6841 gtggattcct cgctcagcta tctgggtgaa tttgccgagg gcggcggcgg cctgccgcag
     6901 ttcccgattt ccgcctatgg ctattcgccg atctcgccca gcctgcagat caacgatttc
     6961 ggcatgaaga ccatcgccgt tttcggccag gccacctaca aggtagacga caagctctcg
     7021 gtcacgctgg gcgggcgcta caaccacgac aagggcacct tcacctccga gcagtatcag
     7081 ggctataccg atgccacgct gacgacgcct ttgtgcaacg tgctggccta tgccgatgcc
     7141 agcgaactgg tgggcgcctg caagatcgcc cgcagcaagc tgtggcaatc cttcacctgg
     7201 aacgccagcc tcaactacaa ggccgatccg cgcacgctgc tctatgtcag cgccgggcgg
     7261 ggctatcagg cgggcggctt caaccagcag atccgggaat cgcaatatcg caccttctcg
     7321 cccgaagtgg tgttcacgct ggaggcgggc ctgaagaagg actggcacct gggcggccgg
     7381 ccgatccgca ccaacatcgc catcttccgt tccaactaca agaaccagca gcgcgtggag
     7441 aacggcacct attccgacgg gctgaacttc atcgccacct tcaacgcggc cagttccacc
     7501 atgtggggcg gcgaggcgga aatcagctat ctgcccaccc cgcagctgga gatctcgctg
     7561 gcctattcct atatcaacgc caaatacgac tatttcaaat cgcccgacgt ggccaatctg
     7621 ggcgggcagg atctcagcag cctggcgatc ggcgccacgc ccaagcacac gttcaacacc
     7681 actgtcagct attgggtgcc caccaacaac cgcgatggca agctgaaggg ctcggtcacg
     7741 gcctatgcgc gctcgggcgc ctattacaac gatctggcgc aaagcgcgct gacctatcag
     7801 gccagctatg cgatcttcaa cgcccgcctc gacctgatca agaccctgcc cttcgatgtt
     7861 ggcgtgtggg tcaacaatct ggccgacaag aactacagcc tgttcaagtt cgacaacctc
     7921 aattcgctgg gttacgcatc ggtgttcctg gcgccgccgc gcaccttcgg cgtggatgca
     7981 tccatccact tctgatccgg gcctgagaga ctgcaaagcc ccgttgcctt tgccggcagc
     8041 ggggcttttg ccgtgccgca aggtggctgg cgccatcgcc ctgccgatgg gtggcggggc
     8101 gggcgcacaa gcgcttcacc ttatgtagca atcactttac aagccgcgcc agatcccctt
     8161 caaccggatg caaagaggag agaggcgcgc catggcaacc cagggcaagg tcaatgaagc
     8221 ggtgcagcac ctgctcgaca ggctggtggc cgaaggcagc gagatcggcg tgcaggtgtg
     8281 cgcctggctg ggcgaggaac agatcgtcga ttgctgggcg gggctggcgg atcctgccac
     8341 cggccgcaag gtggatggcg atacgctgtt caacgtctat tcggtgtcga aagcggtgac
     8401 ggcggtggcg ctgcatatcc aggccgaacg cgggctggtc gattatgacg cgccggtggc
     8461 ccgctactgg ccggaatttg cccaggccgg caagggcgat gtcacggtgc gccacgtgct
     8521 ctcccatgtc tcgggcgtgc tgcgcatgcc gcccgaggtg acgcccgagc tgatgtgcga
     8581 ctgggactgg atgtgccgcc gcattgccga aatggccccc gcctatccgg ccggcagccg
     8641 ttcctcctac cagtcgatga cgtttggctg gctggtgggc gaggtggtgc gccgcacaga
     8701 tccgcagcag cgccccttcg gccgcttcat ccgcgaggaa attgccgatc cgctgggcgc
     8761 caccgatctg tggatgggca ttcccgatgc ggccgaaccg cgcgttgcca aactcgacga
     8821 tgtcgccgtc tatgtcgcgc ccgacaacaa catgatgcgc gaggcccagc cgctggcggt
     8881 ggacctgatg cccgatccct tcgagcggcc cgccgtgcgc cgcgcctgca tcccggcggt
     8941 gggcggcatc ttcaacgccc gcagccaggc gcgcttctgg gcgatgctgg ccaatggcgg
     9001 caggctgggc ggggtgcgcc tgctctcggc cgagcgggtg gccagctttg ccgccccgcg
     9061 cccccatttc gacgatgccg acccggtgtt cttcggcatg caggtgccga tcggctgggc
     9121 cggcttctgg ctgggcgggg cagaggcgcc gccagtatcg gcgccgcgca acatgcgcgc
     9181 cctgtgccac ccgggcatgg gcggctcgat cggctgggcc gatcccgatc tggggctggc
     9241 cgtggccttc tgccacaacc gcatgttcga cacgatcgac attgccgagg acaaccgcac
     9301 gcgcatcggc gatgccatcc gggaagcgct gtgaccatga ccgcaagctg ggattacatc
     9361 atcgtcggcg gcggctctgc cggctgcgtg ctggcagagc gcctctcgcg ccatccggcc
     9421 tgcaaggtgc tgctgct
//
LOCUS       DASRYE010000300        18974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_64856,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000300 DASRYE010000000
VERSION     DASRYE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64856"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1244
                     /gene="lptG"
                     /locus_tag="VFF98_15405"
     CDS             150..1244
                     /gene="lptG"
                     /locus_tag="VFF98_15405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZU65072.1"
                     /translation="MELFPSRTITLYLARLFITRILGVLIMLVLVLQTLDLLSESGTI
                     LAHQGNGEAQIWQYVGLRMPQLVARFLPYSVLLATLFTFFPLNQNSEIIAMRAAGLSA
                     LQILAPLLITAVGVSAFSFLFNETVVTGSTATLKAWQGVNYGRIEQASGVRSNVYIAD
                     GRNILYAETLTGSGAGAHMQDVTWYERDDKGMITRRIESPSASYAAPGWRFDHPQSFD
                     AATTALTELAAPVVVGRGITPRQVDISAVDADGLNIFQLARAIRALHASGRRTAELDG
                     KWWHKVSSPLSAMLMPLLGGVAAFGLARSGQLFVRAVAGMALGFAYFVIDNLALAVGN
                     FGGYPPIIAAWAPFLLFLLVGETVLIRTEE"
     gene            complement(1225..2478)
                     /locus_tag="VFF98_15410"
     CDS             complement(1225..2478)
                     /locus_tag="VFF98_15410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65073.1"
                     /translation="MPDRPLPDTAPEEAPPPLVWLMGLVNMSFGLASAVFIVTLPQLL
                     AEAHVPEPAIANLTAVGLVSGVVALFLAPVLDVGLSRRTYTIALAGLTAAMAFVTLGA
                     LSRLDLLGPLVLALSVSSTLFGSALAGWLGASLPKSADKTLSAWFNIGNGSGFGVGAL
                     LHYQLLGLLPGLWGRLAVAGLILLPLVLLPALPGPTAERQAFRESFGRLAGDLGALLR
                     QKLVLRLLMLFALPCASFALTNLFGGIGGAYHASPVLVDGANGFGVVVISVLGSLIAR
                     PLLDLVPPALLYLLTGSIGACCTLALLALPHTPATYVLAVVVENLFQSAAFVIQYALQ
                     FRSIARGSPLAVTQFALMGAAANLPLAYMQSLDGRGFGQHGVAGAYLVDAGISLAACA
                     AIAPLVWRWWRRGHLGEAAATLPCG"
     gene            2885..3775
                     /locus_tag="VFF98_15415"
     CDS             2885..3775
                     /locus_tag="VFF98_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZU65074.1"
                     /translation="MAQTNLSPAVDNANLPFWKRSHYLDRMTLGELWKAYFQHYTIVA
                     YLALAAVCLVVFGLYPTTALRAAAAVLAAALVYPLVWHMLHQYILHGQWPYKIKILAS
                     TWKRIHYDHHQDPNHLEVLFGALHTTVPTIFFATAPVGALIGGEGGRIGGAAIAFATG
                     LVTTCYYEFMHCIQHLAFKPKWKWVQHMKQRHNEHHYVDEDGNYGITNYWWDHILGTY
                     YEKKDRPGRSPTVFNLGYTEEVAKKYPWVKELSGGIASDHPRRRAIANAQKQAAEADA
                     PTEAQILAHAAEAVGMDTEA"
     gene            3914..5068
                     /locus_tag="VFF98_15420"
     CDS             3914..5068
                     /locus_tag="VFF98_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZU65075.1"
                     /translation="MSAVTITPVTTKAERGQWVDYVYKANAADPAFVPQLRAEELEKV
                     TPGGNPFHEHARCQLFLARRAGQIVGRISAHIDELALGQPLAQGMGPGTGNWGALEAE
                     DAAIASQLIATAEDWLRGQGMARVLAPISLSIWEEPGLLVKGHEQAPTVMMGHQNAKF
                     QPWIEAAGYTPAKSLLTYELDISKPFPPLIQRIVASGEKNPRINVRPVSLKNFAAEAA
                     IIIEILNDAWSDNWGFVPITDSELAYTAKKLKPLIREDLVQIAEYEGEPVAFMMTLPD
                     INEVLKKIGPRPSPLGWIKLLLWIWKPRVRTMRVPLMGVRKRLQSSRMASQLAFMMIE
                     YIRRNSVAHYGASRGEIGWILDDNQGMNSIAREIDSHVNKEYVVYEKALG"
     gene            5417..6220
                     /locus_tag="VFF98_15425"
     CDS             5417..6220
                     /locus_tag="VFF98_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU65076.1"
                     /translation="MPLGSQLAPHIPFLRRYARALTGTAALGDALVRQTLEAALRDDT
                     LRAALAGGRVALYAAFSAQWAHARQHEPLPAPLPAGEGDARAAARWRLSRLAPRQREA
                     LLLTALEDFSPAEAGQILACSPEQVVEMATEARCDLARGAATSVLVIEDEPLIAMQLE
                     ELVRSLGHTICGTAATHAQALRMAETCRPGLVLADIQLADGSSGIDAVEDILAGADLP
                     VIFITAYPERLLTGDRPEPTYLVTKPFREAQVAAAIAQALFFHAPQAAA"
     gene            complement(6227..7906)
                     /locus_tag="VFF98_15430"
     CDS             complement(6227..7906)
                     /locus_tag="VFF98_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HZU65077.1"
                     /translation="MDKIISARVGAQRWYERYPRSVPLGAFVLTMALTLVSVVAMERS
                     NEERERTEMHALANVVASALERRAAGHVAYLRAGAALMANDRTINQAGFMRFAAELRN
                     DAEYRGGQGLTWAPVVPRDKVPAFEAARRAEGMAGYAVHPAPGPAEPLSVPVAYMGEI
                     NPRTRIALGYDMYSDPLRRAAMDEAVRTDRAIATNRVTLMIWQGPNQQAGFVVYLPVY
                     ARGGSRRLLGFIATPFNATAFLDSSVNLRELRGFGVRLYDGEIRPDRLMAEINTRQDS
                     YASTTAPVHLAQRKLMLEVTAEQPDALTPLSWITLAMGTMLALLLMAFARLVTRQAAE
                     DRAALGWLREQASIRASLTRELNHRVKNTLANVLSIIALTRYRATSLADFAESLDGRI
                     RALSATHDLLTASEWAPTPLGAVAAAELAPYATGAPCQVALHGPDVRLAPNDALLFGL
                     AIHELATNACKYGALSQPGGRVTVSWALASAERVSVEWLEEGGPPVSGAHGRGFGMDL
                     LERIVAHELGHSVEMDFRPTGLSCRLAVPVRRPAAFLIRGGARKNTEAESD"
     gene            8119..8364
                     /locus_tag="VFF98_15435"
     CDS             8119..8364
                     /locus_tag="VFF98_15435"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HZU65078.1"
                     /translation="MAGDSPQGSKSTGKAPRKAARATGKAADAQAGGQAGAAPQPCAD
                     RGPAWAAPLRHMYDEVVSEPLPPDLADLLARLDSTGE"
     gene            8361..8927
                     /locus_tag="VFF98_15440"
     CDS             8361..8927
                     /locus_tag="VFF98_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZU65079.1"
                     /translation="MSPPDPFKHELTGAIPHLRAFARGLCGRPDMADDLVQETLLKAW
                     AARERFIPGTSIRAWTFTILRNLYLTDRRRSRNQGEYDELVAERTLITPAGQEGPLHL
                     ADLRRALMQLPPERREALLLVGAGGFSYEEAAAICGCAVGTIKSRVGRARAALATMIE
                     QGVEGPREGGQAGDAIMADLARVSERAS"
     gene            9021..10361
                     /gene="glmM"
                     /locus_tag="VFF98_15445"
     CDS             9021..10361
                     /gene="glmM"
                     /locus_tag="VFF98_15445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZU65080.1"
                     /translation="MGRQYFGTDGIRGRTNAGVMNAEIAMKVGQAAGTHFLRGRHRHR
                     VVIGKDTRLSGYMLENAMVAGFTSVGMDVVLLGPLPTPAIALLTRELRADVGVMISAS
                     HNPYEDNGIKLFGPDGFKLSDEDEEAIEAMLEQRLPLAASEEIGRARRIEDARGRYIH
                     AVKGSLPHNVRLDGLKIVVDCANGAAYQVAPSAIWELGAEVISLGVNPNGKNINDRCG
                     STHVDLLQETVVASGADIGIALDGDADRLIVVDEKGQTVDGDQIMALIGSQLAARGEL
                     RGGGVVATVMSNLGLERFLTGRGLTLERTKVGDRHVLERMRAGGFNVGGEQSGHMILL
                     DHATTGDGTIAALQVLAALVQSGKRASELLHLFDPVPQLLKNVRFSGGKPLEAAAVKQ
                     VIAEAEGELAGRGRLVIRPSGTEPVIRVMAEGDDEAQVTQVVDAICEAVRLAAA"
     gene            10367..10591
                     /locus_tag="VFF98_15450"
     CDS             10367..10591
                     /locus_tag="VFF98_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HZU65081.1"
                     /translation="MLDMRPDCETCGTALPAEAPGAFICSLECTFCAECADGHDELCP
                     NCGGELMDRPVRAKKLQAKYPPSTKRHFKG"
     gene            10588..11352
                     /gene="thiD"
                     /locus_tag="VFF98_15455"
     CDS             10588..11352
                     /gene="thiD"
                     /locus_tag="VFF98_15455"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZU65082.1"
                     /translation="MSRSPGILAPRILTIAGSDCSGGAGLQADLKTITMLGGYGMSAI
                     TAITAQNTLGVFAVEALSPAMVAAQIDACISDIGVDAVKIGMLGSAAIAHAVADRLEQ
                     IAAPVVFDPVMVATSGGVLADAETVAAFARLMARADLTTPNLPELAALGGEEAVLGRT
                     RALLVKGGHGTGEEICDRLLIDGCDYEWTTQRQHTRHTHGTGCTLASAIATGLGAGMD
                     MPDAVEQAIAFVAAALAAAPGYGAGCGPLGHQAVRL"
     gene            complement(11349..12239)
                     /locus_tag="VFF98_15460"
     CDS             complement(11349..12239)
                     /locus_tag="VFF98_15460"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZU65083.1"
                     /translation="MTEEEKALVRRRFYRAEQEASFVEAHVTHTPQTLSPSYKLAFRD
                     TEFLLREELRPVRFQLELLKTEMLLDEAGIGSTLVCYGSARIPAPEETEERLAAATTP
                     EEKLRAERLAAKARYYDEARQLARIASQCAIVEKGMRQFVICSGGGPSIMEAANRGAA
                     EVGAESIGLNIVLPHEQAPNRFVTPRLAFQFHYFALRKMHFLLRARAVAVFPGGFGTF
                     DEFFELLTLIQTGKMKPIPILLFGAEFWNKVVNFSALAEEGVVSEKDLALFTFVETAE
                     EAWAHIGQFYGFDCWPDGDS"
     gene            12467..13213
                     /locus_tag="VFF98_15465"
     CDS             12467..13213
                     /locus_tag="VFF98_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HZU65084.1"
                     /translation="MALAVALAGAGVAHAQMGGMGGMGGGGMGGMGGGGGGMGGGPPS
                     GGGDEAPADKGPPKPHVMKPIPQKKFDEAVTQMFAAADTNHDGLITLAEFQAQVEARR
                     DAIIRARFQKIDLNHDGQISQEEFISWQLSLGSMASADTGGRANVEVVAEEIGPKLGN
                     SEQDMALAMAIEPINPVMLVKANTNYDAGVSLAELLAYEDARFAKADTDRDGYLTMEE
                     IRAMMPARGPGGRGPGGRGPGGMMPPPGDR"
     gene            complement(13245..14330)
                     /locus_tag="VFF98_15470"
     CDS             complement(13245..14330)
                     /locus_tag="VFF98_15470"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU65085.1"
                     /translation="MATAAPAPETLAPQTPAEAPTPPAGCTFDAGNWAILARHWYPVA
                     TLEEVTQGPVKARLLDQPLVVYRAGGELVVAPDLCPHRGVPLSLGHQGEKGVVCPYHG
                     LTFGAKGHCVHVPAQPARDISPRFHLKTFPAIARYGLIWTCLAPDAQETASGQPAAGA
                     IIDLPHADEEGFQHIVCPPFDIKGYAGRQLEGFLDVAHFAFIHTATFADPENAAVPDY
                     RPRQTPFGFEAEYWSTVGNYPHSSGLAPEGFRWLRHFRCQAPFTATLEVHFPGSHRLV
                     ILNAASPVSARATRLFVPILRNYDTHLPVADVHAFNLAVFAEDAAMVEAQSPAHVPLD
                     PTAEAHLPADMSSMFYRRALKEMGLTG"
     gene            14506..15729
                     /locus_tag="VFF98_15475"
     CDS             14506..15729
                     /locus_tag="VFF98_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZU65086.1"
                     /translation="MRSVGLIAVPVLAVLGLLAGFALSLSAASRAFEHGLIAQRQAAL
                     VEAIAADADRLDQASLQRRLAAYRALVVEERPFLSAADAAAQGAEQRRAAALSALAGV
                     AADRPRMAALVAAIAAEEAHEVAATRAGLARLRRNMVVLGALLAAAALGAVAAGALQM
                     LRANRDLAGQVAERTAALEAVDASRRLFFAKASHELRTPVTAIRTIAEVAQEGADQAQ
                     ALADIVAQAGFLEHRIADLMALAAAGEGQPALVLAPGDLAGVIAGAAAQAGPYARSLG
                     VEIVVTAPAAPLIARIDGDRLAQALLAVIDNGLKFSDPGARLELALEAASGQAVLAVA
                     DSGPGVLPPELPRIFDAYYQAGAGRARGGSGLGLALARWVVERHGGTILAENRVGGGC
                     RIVIGLPLVAEGEGQ"
     gene            15726..16397
                     /locus_tag="VFF98_15480"
     CDS             15726..16397
                     /locus_tag="VFF98_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU65087.1"
                     /translation="MILLVEDDVAIGRSLAQGLAARGFAVRWLRQAAQAAGALDTGEV
                     AVAVLDLGLPDGDGLALCRQWRAAGHRLPVLMLTARAALDDRLEGFAAGADDYLPKPF
                     AFAELAARVEVLARRAAQLPAAPVLWGPFAFDSAAGQVLRDGAPLAFEPKALALLLRL
                     AQARGGVVARDALIDAGWGEMAAVGGNTLDVAISALRRRLADVAPELAVKAVKGQGYQ
                     LDLIP"
     gene            16486..>18974
                     /locus_tag="VFF98_15485"
     CDS             16486..>18974
                     /locus_tag="VFF98_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65088.1"
                     /translation="MHNKGMRLRGASLAVMAVLAGTQARGAEANAANGANGDPIIVVT
                     APIATDTARLDDVPANAQVLAGDALGRQNHANLADLLNANLGSVSLSNGSGSPYQSDV
                     AYRGFQATSLLGSPTGLSVWLDGVRMNEAFGAIVNWDLIPMNAVSAVEVLPGSNPVFG
                     LNTLGGALVLTTKNGADNGGLSIMAEGGSFNRRSVAAEAGGTAANKAIDWFLAGNYDE
                     QDGYRLHTHTRVEQLYGKLRWHGAGANAELGAMWSASQLNGTQSLPLSMLSTPEASYT
                     WPDSVANHQLLINLKADASLAAGLKISGNVYFRRSNASGVNSNASLDDNCGESRDCSA
                     SAPGGTAVDLYQTNPYLAGSARANGFVAYAGTLPIHDYTDNINTSLVFSSTRQHSWGG
                     NLLLDSDAPLLGRKNDLNLGGSFEIADITYDQSTYLAYLVGYQTVQMPWNFKYGSAGG
                     FEGSPLVNSVDVASHSASFNLFVRDTLKLTETLGLTASLSYTSTHVSLGGTNSTFLNE
                     DGGFSWTGADGQKYYNPAYIGASYWTTPSSGSSRLATASIPTGGVAGPEVVAVTGAHL
                     YHRFNPAVGLAWNPLPALGLFASYSEAMRAPTAIELACADPATPCALPTGFNGDPDLK
                     AVVARTVEVGARGRVGGHVSWNAALYRTGLTNDIQFIYDTSGLGYFANVGRTERRGLE
                     LGLAADWARLHLSASYGHVVATYRSTFTDASGDTVQAGNHIPGIPADSFKLRAVYAPT
                     KALALGVNLIAVSSQWAHGDEANLYGAVPGYALVNADLHVVPRPGWELFANITNLFNR
                     HYATYGVMGTNIYTGNDEQFRTPAQGRAFVAG"
BASE COUNT         3081 a         6206 c         6711 g         2976 t
ORIGIN      
        1 tcgcggtgat catcggctgg atgtattggc gcattgccta tgtgccgggc gggcagccga
       61 ttggcgggct ggagaaattc gcgcaaatgg tgtggcgggg cctggcgcgc ggggccaggc
      121 gcctgcgcca gctggtgggc ggataggcca tggaactgtt tccctcgcgc acgatcacgc
      181 tgtatctggc gcggctgttc atcacccgca tcctgggcgt gctgatcatg ctggtgctgg
      241 tgctgcagac gctggacctg ctgagcgaat cgggcacgat cctggcgcat cagggcaatg
      301 gcgaggcgca gatctggcaa tatgtgggcc tgcgcatgcc ccagctggtg gcgcgcttcc
      361 tgccctattc ggtgttgctg gcaacgctgt tcaccttctt cccgctgaac cagaacagcg
      421 agatcatcgc catgcgggca gcgggcctgt cggcgctgca gatcctggcg ccgctgctga
      481 tcacggcggt gggcgtctcg gccttcagct tcctcttcaa cgagacggtg gtgaccggtt
      541 ccaccgccac gctgaaggcc tggcaggggg tcaactatgg ccggatagag caggccagcg
      601 gcgtgcgcag caatgtctac atcgccgacg ggcgcaacat cctctatgcc gaaacgctga
      661 ccggcagcgg cgccggcgcg catatgcagg atgtcacctg gtatgagcgt gacgacaagg
      721 ggatgatcac ccgccgcatc gaaagcccgt cggccagcta tgcggcgccg ggctggcgct
      781 tcgatcaccc gcaaagcttc gatgcggcca ccaccgcgtt gacggaactg gccgcgccag
      841 tggtggtggg ccggggcatc acgccgcggc aggtggatat cagcgcggtg gatgccgatg
      901 ggctgaacat tttccagctg gcccgcgcca ttcgtgcgct gcatgccagc ggccgccgca
      961 cggccgagct ggacggcaaa tggtggcaca aggtgtcgag cccgctgtcg gccatgctga
     1021 tgcccttgct gggcggcgtt gcggcctttg gcctggcgcg ttcgggccag ctgtttgtgc
     1081 gcgcggtggc gggcatggcg ctgggctttg cctatttcgt gatcgacaat ctggcgctgg
     1141 cggtgggcaa tttcggcggc tatccgccga tcatcgcggc ctgggcgccg ttcctgctgt
     1201 tcctgctggt gggcgagact gtgctcatcc gcacggaaga gtagccgccg cctcgcccag
     1261 gtgcccgcgg cgccaccagc gccacaccag cggcgcgatg gcagcgcaag cggcaaggct
     1321 gatgccggca tccaccagat aggcgccggc cacgccatgc tggccaaagc cgcgcccatc
     1381 cagtgattgc atataggcca gcggcaggtt ggccgccgcc cccatcagcg cgaattgcgt
     1441 gaccgccagc gggctgcccc gcgcgatgga gcggaattgc agcgcatact ggatgacgaa
     1501 ggcggccgac tggaacaggt tttccaccac caccgccagc acataggtgg ccggcgtgtg
     1561 cggcagcgcc agcagcgcca gcgtgcagca ggcgccgatg ctgccggtga gcagatacag
     1621 cagcgccggc ggcacaagat cgagcagcgg gcgggcgatg aggctgccca gcaccgagat
     1681 caccaccacg ccaaagccat tggccccatc caccagcacc ggcgaggcat gataggcccc
     1741 gccaatgccg ccaaacaggt tggtgagcgc gaaagaggcg cagggcaggg caaacagcat
     1801 cagcagccgc agcaccagct tctggcgcaa gagcgcgccc agatcgcccg ccagccggcc
     1861 aaagctctcg cgaaaggcct gccgctcggc cgtggggccg ggcagcgccg gcagcagcac
     1921 cagcggcagc aggataagcc ctgccaccgc cagccggccc cacaggcccg gcagcaggcc
     1981 cagcagctgg taatgcagca gcgcgcccac gccaaagccg ctgccattgc cgatgttgaa
     2041 ccaggcgctc agcgtcttgt cggcgctttt gggcaggctg gcgcccagcc agccggccag
     2101 cgcgctgccg aacagggtgg aggagaccga cagcgccagc accagcggcc ccagcagatc
     2161 cagccgcgag agcgctccca gcgtgacaaa ggccatggcc gccgtcagcc cggccagggc
     2221 aatcgtatag gtgcgccggc tgaggccgac atcgagcacc ggcgccagaa acagcgccac
     2281 cacgcccgac accagcccca ccgcggtgag attggcgatg gccggttccg gcacatgggc
     2341 ctctgccagc aattgcggca gggtgacgat gaacaccgcg ctcgccaggc caaagctcat
     2401 gttcaccagc cccatcagcc agacgagcgg cggcggcgct tcctcgggcg cggtgtctgg
     2461 cagcgggcga tcgggcatgg cggggctttc acggcaggag gagggggcgc aggaggaggg
     2521 ggcaagggct atcggccggc ggggcagggg tcaaccgcgt ggctgcccca atcccgccgc
     2581 aatcacggcc taagggcgca agcctccccc acgcgcgggg aaaggccggc cgcaaggcct
     2641 gccgctgccg tctcgtctgg cggcatggca ggctctgctg gtcgattttt tcaccgcgct
     2701 ttgacatgcg acaaggatgg ggcggggtgc tttcgggctt gggcgatgcc ggcgttcgcg
     2761 ttttcggttc ttccgccccg cccgcatcgc gcccgctcca ccagagggca tgtccggcgg
     2821 gcggtcttgc cgcccctcgg gcttgccttt atgagaacct ttcgcacaga caggatcagg
     2881 acccgtggcc cagaccaatc tttcgccggc ggtggataac gccaatctgc ccttctggaa
     2941 acgctcgcac tatctcgacc ggatgaccct gggcgagctg tggaaggcct atttccagca
     3001 ctataccatc gttgcctatc tggcgctggc ggcggtgtgc ctggtggtgt tcggccttta
     3061 tcccaccacg gcgctgcggg cggcggcggc ggtgctggcg gcggcgctgg tctatccgct
     3121 ggtgtggcac atgctgcacc agtatatcct gcatggccag tggccctaca agatcaagat
     3181 cctggccagc acctggaagc gcatccatta cgaccaccac caggatccca accacctgga
     3241 agtgctgttt ggcgcgctgc acaccaccgt gccgacgatc ttctttgcca cagcgcctgt
     3301 gggcgcgctg atcggcggcg agggcgggcg catcggcggg gcggcgatcg cctttgccac
     3361 cggccttgtc accacctgct actacgagtt catgcattgc atccagcacc tcgccttcaa
     3421 gccgaagtgg aagtgggtgc agcacatgaa gcagcgccac aacgagcacc actatgtgga
     3481 tgaagacggc aactatggca tcaccaacta ctggtgggac catatcctgg gcacctacta
     3541 tgaaaagaag gaccgccccg gccgcagccc caccgtgttc aacctgggct acaccgagga
     3601 agtggcgaag aaatacccct gggtgaagga actgtcgggc ggcatcgcca gcgatcatcc
     3661 gcgccggcgc gccattgcca acgcccagaa gcaggccgcc gaggccgacg cccccaccga
     3721 ggcccagatc ctggcccatg ccgcagaggc cgtggggatg gacaccgagg cctgagggct
     3781 tccccggccc cgttcacgca tcatcgggca tttgcgcgca ggggtccttt gcgcgcaaat
     3841 gcctttttct tgccgccatc ggccgctaca caccccccgc gccactgccg ctaccgcttg
     3901 gggagtatct agcatgtctg ccgtcaccat cacgccggtc accaccaagg ccgagcgcgg
     3961 ccagtgggtc gattacgttt acaaggccaa tgccgcagat cccgccttcg tgccgcaatt
     4021 gcgcgccgaa gagctggaga aggtgacgcc gggcggcaac ccgttccacg aacatgcccg
     4081 ctgccagctg ttcctcgccc ggcgcgcggg ccagatcgtt ggccgcattt cggcgcatat
     4141 cgacgagctg gcgctgggcc agccgctggc gcagggcatg gggccgggca ccggcaactg
     4201 gggcgcgctg gaagccgagg atgccgccat tgccagccag ctgatcgcca cggcggaaga
     4261 ctggctgcgc ggccagggca tggcccgcgt gctggcgccg atcagccttt ccatttggga
     4321 ggaaccgggc ctgctggtga agggccatga gcaggcgccg acggtgatga tgggccacca
     4381 gaacgccaag ttccagccgt ggatcgaggc ggcgggctat acgccggcca agagcctgct
     4441 gacctatgag ctggatattt ccaagccctt cccgccgctg atccagcgca tcgtcgccag
     4501 cggcgagaag aacccgcgca tcaatgtgcg cccggtgagc ctgaagaatt tcgcggccga
     4561 ggcagcgatc atcatcgaga tcctcaacga tgcctggtcg gacaactggg gctttgtgcc
     4621 gatcaccgat tcggagctgg cctatacggc caagaagctg aagccgctga tccgcgaaga
     4681 cctcgtgcag atcgccgaat atgagggcga gccggtggcc ttcatgatga ccctgcccga
     4741 tatcaacgag gtgctgaaga agatcggccc gcgcccctcg ccgctgggct ggatcaagct
     4801 gctgctgtgg atctggaagc ccagggtgcg caccatgcgc gtgccgctga tgggggtgag
     4861 gaagcgcctg caatccagcc gcatggccag ccagctggct ttcatgatga tcgaatatat
     4921 ccgccgcaat tcggtggccc attatggcgc cagccgcggc gagatcggct ggatcctcga
     4981 cgacaaccag gggatgaact ccatcgcccg tgagatcgac agccatgtga acaaggaata
     5041 tgtcgtctac gagaaggcgc tgggctgagg cggcccgggg gggcgatgcc ccccgccagc
     5101 cttgcctggc tgaaagcaga tacaaaaagg cccccgctcg ggggaacggg ggcctttccg
     5161 ggatcgcgtc accaccggtt tggacgggag ggggggtctc ggtggtgaca aagggataac
     5221 gcggggccct gacagaggtt cccgcttttg cgaaaaattt tcgacagccc tgcaacatca
     5281 tgaaaaaagg ctgtaaactt ttgccgcgcg gcggcgaggg aaccaaaccg gccgggtggc
     5341 gttggcccgg tggcggtgtt acatgccgat ggccgccgcg caatcatccg cgaccatccg
     5401 ctgaggagcc cgtttcatgc cgcttggcag ccagcttgcc ccccatattc ctttcctgcg
     5461 ccgctatgcg cgcgcgctca cgggcacagc ggcgctgggc gatgcgctgg tgcgccagac
     5521 gctggaagcg gccctgcgcg acgatacctt gcgcgccgcg ctggctggcg ggcgggtggc
     5581 gctgtatgcc gccttcagcg cgcaatgggc ccatgcccgc cagcacgagc cgctgcctgc
     5641 cccgctgcct gcgggcgagg gggatgcaag ggcggcggcg cgctggcgcc tctcgcgcct
     5701 ggccccgcgc cagcgcgagg cgctgctgct gaccgcgctg gaagacttca gccccgccga
     5761 ggccggccag atcctggcat gcagccccga acaggtggtg gaaatggcca ccgaggcgcg
     5821 gtgcgatctg gcccggggcg ctgccaccag cgtgctggtg atcgaggatg aaccgctgat
     5881 cgccatgcag ctggaagagc tggtgcgcag ccttggccac accatctgcg gcaccgcggc
     5941 cacccatgcc caggccctgc gcatggccga aacctgccgg ccggggctgg tgctggccga
     6001 tatccagctg gccgatggca gttcggggat cgatgcggtg gaagatattc tggccggtgc
     6061 cgatctgccg gtgatcttca tcaccgccta tcccgaacgg ctgctgaccg gcgaccggcc
     6121 cgagccgacc tatcttgtca ccaagccctt ccgcgaggcg caggtggccg ccgccattgc
     6181 ccaggcgctg ttcttccacg cgccccaggc ggccgcatag ggctgcctag tcgctctccg
     6241 cctcggtgtt cttgcgggcg ccgccgcgga tgaggaaggc cgccgggcgg cgcaccggca
     6301 ccgccagccg gcagctgagg ccggtggggc gaaagtccat ctccacgctg tggcccagct
     6361 catgggcgac gatgcgctcc agaagatcca tgccaaagcc gcgcccatgc gcgcccgaga
     6421 ccggcggccc gccctcttcc agccattcca ccgacacgcg ctcggcactg gccagcgccc
     6481 agctgacggt aacgcgcccg cccggctggc tcagcgcgcc atatttgcag gcgttggtgg
     6541 ccagctcgtg gatggccagg ccaaacagca gcgcatcatt gggggccagg cgcacatccg
     6601 ggccatgcag cgccacctgg cagggcgcgc cggtggcata gggcgccagt tcggccgctg
     6661 ccaccgcgcc cagcggggtg ggcgcccatt cgctggcggt cagcagatcg tgggtggcgg
     6721 aaagggcgcg gatgcggcca tcgaggcttt ccgcaaaatc ggccaggctg gtggcgcgat
     6781 agcgggtgag ggcgatgatc gagagcacat tggccagcgt gttcttcacc cggtggttca
     6841 gctcacgcgt cagcgaggcg cggatcgagg cctgttcgcg cagccagccc agcgctgccc
     6901 ggtcttccgc cgcctggcgg gtgacaaggc gggcaaaggc catcagcagc agcgccagca
     6961 tggtgcccat cgccagcgtg atccacgaca gcggggtcag cgcgtcgggc tgctcggcgg
     7021 tcacttccag catcagcttg cgctgggcca gatgcaccgg cgctgtggtg ctggcatagc
     7081 tgtcctgccg ggtgttgatc tcggccatca gccggtcggg gcggatctcg ccatcgtaga
     7141 ggcgcacgcc aaagccgcgc aattcgcgca ggttcaccga ggaatcgagg aaggcggtgg
     7201 cattgaacgg cgtggcgatg aagcccagca gccgccggct gcccccccgc gcatagaccg
     7261 gcagatagac gacaaagccc gcctgctggt tgggcccctg ccagatcatc agcgtgacgc
     7321 ggttggtggc gatggcccgg tcggtgcgca cggcctcgtc catggcggcg cggcgcaggg
     7381 ggtcggaata catgtcgtag cccagcgcga tgcgggtgcg cgggttgatc tcgcccatat
     7441 aggccaccgg cacgctcagc ggctctgcgg ggccgggggc ggggtgcacg gcatagccgg
     7501 ccatgccctc ggctctgcgg gcggcctcga aggcgggcac cttgtcgcgc gggaccaccg
     7561 gcgcccaggt gaggccctgg ccgccgcgat attcggcatc gttgcgcagc tctgcggcaa
     7621 agcgcatgaa gccggcctgg ttgatggtgc gatcattggc catcagcgcg gcgcccgcgc
     7681 gcagataggc gacatggcct gccgcccgcc gttccagcgc ggaagccacg acattggcca
     7741 gcgcgtgcat ctcggtgcgc tcgcgctcct cgttcgagcg ctccatcgcc accacgctca
     7801 ccagcgtcag cgccatggtc agcacgaaag cgcccagcgg cacgctgcgc ggatagcgtt
     7861 cataccagcg ctgggcgccc acgcgtgccg aaatgatctt gtccacgctg tgcgccccgc
     7921 cggatttcgc tgctttgtcc atggggccta gcgacaatcc agatggaagg gaaccttgtg
     7981 ggaaccacgg ggcgccagtt tcgttccctt tggcgaatgc ttcccgtttg cccagaggaa
     8041 aacgccttgc caccccccga accacctttg cgatggacga gatggacagg cgtgcgcagc
     8101 tggtacaaca aggcacatat ggcaggcgat tccccccaag gctccaagag caccggcaag
     8161 gccccgcgca aggcggcaag agccaccggc aaggctgcag atgcgcaggc cggtgggcag
     8221 gctggcgccg cgccgcagcc ctgcgccgat cgcgggccgg cctgggccgc gcccttgcgc
     8281 cacatgtatg acgaggtggt gagcgagccg ctgccgcccg atctggcgga tctgctggcc
     8341 cggctggaca gcaccggcga atgagcccgc ccgacccctt caagcacgag ctgacagggg
     8401 cgatccccca tctgcgcgcc tttgcccgcg gcctgtgcgg ccggcccgac atggccgatg
     8461 atctggtgca ggaaacgctg ctgaaggcct gggccgcgcg cgagcggttc attcccggca
     8521 cctcgatccg cgcgtggacc ttcaccatcc tgcgcaacct ctatctcacc gaccggcgcc
     8581 gctcgcgcaa tcagggcgaa tatgacgagc tggtggccga gcgcacgctg atcaccccgg
     8641 ccggccagga agggccgctg cacctggccg atctgcgccg ggcgctgatg cagctgccgc
     8701 ccgaacggcg cgaggcgctg ctgctggtgg gcgcgggcgg cttttcttac gaggaagcgg
     8761 cggccatctg cggctgcgcg gtgggcacca tcaagagccg ggtgggccgc gcgcgcgccg
     8821 cgctggcaac gatgatcgag cagggcgtgg aaggcccgcg cgagggcggg caggccggcg
     8881 atgccatcat ggccgatctg gcgcgggtga gcgaaagggc aagctagccg ccggcgccgc
     8941 ctccccccgt gacaaacgcc cgctttggcg acataggacg gcaagcgccc ggcatggcgc
     9001 ggccaacaag gggttttgcg atgggcaggc agtatttcgg caccgatggc attcgcgggc
     9061 ggaccaacgc cggcgtgatg aacgccgaaa tcgccatgaa ggtgggccag gcggcgggca
     9121 cccatttcct gcgcgggcgg caccgccacc gcgtggtgat cggcaaggac acgcgcctgt
     9181 cgggctatat gctggaaaac gcgatggtgg ccggcttcac cagcgtgggc atggatgtgg
     9241 tgctgctggg ccccttgccc acgcccgcca ttgccctgct cacccgcgaa ttgcgcgccg
     9301 atgtgggggt gatgatctcg gccagccaca atccctatga ggataatggc atcaagctgt
     9361 tcgggccgga tggcttcaag ctgtcggacg aggatgagga agcgatcgag gcgatgctgg
     9421 agcagcgcct gccgctggcg gcttcggaag agatcggccg ggcgcggcgc atcgaggatg
     9481 cgcgcgggcg ctatatccat gcggtgaaag gctcgctgcc gcacaatgtg cggctggatg
     9541 ggctgaagat cgttgtcgat tgcgccaatg gcgcggccta tcaggtggcg cccagcgcga
     9601 tctgggaact gggcgcggaa gtgatcagcc tgggggtgaa ccccaatggc aagaacataa
     9661 atgatcgctg cggctccacc catgtcgatc tcttgcagga aacggtggtg gcatcgggcg
     9721 cggatatcgg cattgcgctg gatggcgatg ccgaccggct gatcgtggtg gacgagaagg
     9781 gccagacggt ggacggcgac cagatcatgg cgctgatcgg cagccagctg gccgcgcggg
     9841 gcgaattgcg cggcggcggc gtggtggcca cggtgatgtc gaacctgggg ctggagcgtt
     9901 tcctcaccgg ccgcggcctc acgctggagc gcaccaaggt gggcgaccgc cacgtgctgg
     9961 aacgcatgcg cgccggcggc ttcaatgtgg gcggcgagca atcgggccac atgatcctgc
    10021 tcgaccatgc gacaaccggc gatggcacga tcgccgcgct gcaggtgctg gcggcgctgg
    10081 tgcaatcggg caagcgggcg agcgagctgc tgcacctgtt cgatccggtg ccgcagctcc
    10141 tcaagaatgt gcgcttttcc ggtggcaagc cgctggaggc ggcggccgtg aagcaggtga
    10201 tcgccgaggc cgagggcgag ctggccgggc gcggccgcct ggtcatccgc ccctcgggca
    10261 cggaacctgt catccgcgtg atggccgagg gcgatgatga ggcgcaggtg acgcaggtgg
    10321 tcgatgccat ctgcgaggcc gtgcggctgg cggcggcctg atcgccatgc tggacatgcg
    10381 ccccgattgc gagacttgcg gcaccgccct gccggccgaa gcgcccggcg ccttcatctg
    10441 cagcctggaa tgcaccttct gcgccgaatg cgccgacggg catgacgagc tgtgccccaa
    10501 ttgcggcggc gagctgatgg accggccggt gcgggcgaag aagctgcagg cgaaataccc
    10561 cccttccacc aagcgccact tcaagggatg agccgctcgc ctggcattct ggccccccgc
    10621 atcctgacga tcgccggttc cgattgcagc ggcggcgccg gcctgcaggc agacctcaag
    10681 accatcacca tgctgggcgg ctatggcatg agcgccatca ccgccatcac cgcgcagaac
    10741 acgctgggcg tgtttgcggt ggaggcgctg tcgccggcga tggtggcagc gcagatcgat
    10801 gcctgcatca gcgatatcgg cgtggatgcg gtgaagatcg gcatgctggg cagcgcggcc
    10861 atcgcccatg ccgtggccga ccggctggag cagattgccg cgcccgtggt gttcgatccg
    10921 gtgatggtgg ccaccagcgg cggcgtcttg gccgatgccg aaaccgtggc cgcctttgcc
    10981 cgcctgatgg cgcgggcaga ccttaccacc cccaacctgc ccgaactggc agcgctgggc
    11041 ggcgaggaag cagtgctggg ccgcacccgc gcgctgctgg tgaagggcgg ccatggcacc
    11101 ggcgaggaga tctgcgaccg cctgctgatc gatggctgcg actatgaatg gacgacgcag
    11161 cgccagcaca cccgccacac ccatggcacc ggctgcacgc tggccagcgc cattgccacc
    11221 ggccttggcg ccggcatgga catgcccgat gcggtggaac aggcgatcgc ctttgtggcc
    11281 gcagcccttg ccgcggcgcc gggttatggc gccgggtgcg ggccgctggg ccatcaggcg
    11341 gtcaggcttt agctgtcccc gtcgggccag caatcaaagc cgtagaactg gccgatatgg
    11401 gcccaggctt cctcggccgt ttcgacaaag gtgaacagcg ccagatcctt ttccgagacg
    11461 acgccctctt cggccagcgc gctgaagttc accaccttgt tccagaattc ggcgccgaac
    11521 agcaggatcg ggatcggctt catcttgccg gtctggatca gcgtcagcag ctcgaagaat
    11581 tcgtcgaacg tgccaaagcc gccggggaac accgccaccg cccgcgcgcg cagcaggaaa
    11641 tgcatcttcc tcagcgcgaa atagtggaac tggaaggcca ggcggggcgt gacgaagcgg
    11701 ttgggcgcct gttcatgcgg cagcacgatg ttgaggccga tgctttctgc ccccacttcg
    11761 gccgcgcccc ggttggccgc ttccatgatc gaggggccgc cgcccgagca gatgacgaac
    11821 tggcgcatcc ccttttccac aatcgcgcac tggctggcga tgcgggccag ctggcgggcc
    11881 tcgtcgtaat aacgggcctt ggcggccagg cgttcggcgc gcagcttttc ctcgggcgtg
    11941 gtggccgcgg ccaggcgctc ttcggtctct tcgggcgcgg ggatgcgggc cgagccatag
    12001 cacaccagcg tggagccgat gcccgcctca tccagcagca tttccgtctt cagcagttcc
    12061 agctggaagc gcaccgggcg caattcctcg cgcagcagga attcggtgtc gcggaaggcc
    12121 agcttgtagg aggggctgag cgtttgcggt gtgtgggtga catgtgcctc gacaaaggag
    12181 gcttcctgct cggcgcgata gaagcgccgg cgaacgaggg ctttttcttc ttctgtcatg
    12241 ctcgtgtcta taattggctt gggggacggc gatagcatga aaagcgtgaa tcctgcgtga
    12301 ccggccgctt gcggtggcaa acggggcggg gctggcggct ttttgttcat cctgcagcaa
    12361 ggcagggctg cacacacggc aacttgtcgg gtacgacagg atggccgggc gggggcggcg
    12421 ccagcttagg ggaaatcgac cagtgaacag gatgatgaca ggcctggtgg ccttggctgt
    12481 cgcgcttgcg ggcgccggtg tggcccatgc ccagatgggt ggcatgggcg gcatgggtgg
    12541 cggcggcatg ggcggtatgg gcggcggcgg tggcggcatg ggcggcggcc cgccttcggg
    12601 cggcggcgac gaggccccgg ccgacaaggg cccgcccaag ccgcatgtga tgaagccgat
    12661 cccgcagaag aagttcgacg aggcggtgac gcagatgttt gccgcggccg acaccaacca
    12721 cgatggcctc atcaccctgg ccgaattcca ggcgcaggtg gaggcgcggc gcgatgccat
    12781 catccgcgcc cgcttccaga agatcgacct caaccatgat ggccagatca gccaggagga
    12841 attcatcagc tggcagctca gcctcggctc catggcttct gccgacaccg gcgggcgcgc
    12901 caatgtggaa gtggtggccg aggagatcgg cccgaagctg ggcaacagcg agcaggacat
    12961 ggcgctggcc atggcgatcg agccgatcaa cccggtgatg ctggtgaagg ccaacaccaa
    13021 ctatgatgcc ggcgtgtcgc tggcagagct gctggcctat gaggatgccc gcttcgccaa
    13081 ggccgatacc gaccgcgatg gctatctcac catggaagag atccgcgcga tgatgccggc
    13141 gcgtgggccc ggtggccggg gcccgggcgg gcgcgggccc ggcggcatga tgcccccgcc
    13201 gggcgatcgc tgaaccaaag gccccggccc gcttgcggcc ggggtcagcc tgtcagcccc
    13261 atctccttca gcgctcggcg atagaacatc gagctcatgt cggcgggcag atgggcctcg
    13321 gcggtggggt ccagcggcac atgggccggg ctttgcgctt ccaccatggc ggcatcctcg
    13381 gcaaatacgg caaggttgaa ggcgtgcaca tcggccaccg gcaggtgggt gtcgtaattg
    13441 cgcaggatcg gcacgaacag ccgcgttgcg cgcgcggaaa ccggcgaggc ggcgttgagg
    13501 atcaccagcc ggtgggatcc ggggaaatgc acttccagcg tggcggtgaa gggcgcctgg
    13561 cagcggaaat ggcgcagcca gcgaaagcct tcgggggcaa ggccgctgct gtgcggataa
    13621 ttgcccaccg tgctccaata ttccgcctcg aagccgaagg gggtttgccg ggggcgatag
    13681 tcgggcacgg cggcgttttc gggatcggca aaggtggcgg tgtggatgaa ggcgaaatgc
    13741 gccacatcga gaaagccttc cagctgccgc ccggcatagc ccttgatgtc gaagggcggg
    13801 cagacgatgt gctggaagcc ctcctcgtcg gcatggggca gatcgatgat cgcgccggcc
    13861 gccggctggc cagaggcggt ctcctgcgca tcgggggcaa ggcaggtcca gatcaggcca
    13921 taacgggcga tggcggggaa ggtcttgagg tggaagcggg gggaaatgtc gcgcgcgggc
    13981 tgggcgggca catgcacgca atggcccttc gcgccgaagg tcaggccatg ataggggcac
    14041 accacgccct tctcgccctg atggcccagg ctgagcggca cgccgcgatg ggggcacaga
    14101 tcgggcgcca ccaccagctc tcccccggcg cgatagacga ccagcggctg atcgagcagc
    14161 cgcgccttca cgggcccttg cgtcacctct tccagcgtgg ccaccggata ccagtggcgg
    14221 gccaggatcg cccagttgcc ggcatcgaag gtgcagccgg cgggcggggt gggggcttca
    14281 gcaggggttt ggggcgcaag ggtttcaggg gcgggcgcgg cggtggccat gggctctctc
    14341 tttttcgcgg ggggatcgga tgggcgcagg atgccagccc gccagtgcgc tatcaaacgc
    14401 aatgatcctg acagggcgtt gcgaaaaagc gcgcatcctt catcgccctt gccgccgggg
    14461 gcccggctgc gccatcctgc ggcacaggca agggggcggg cgggcatgcg ttctgtgggg
    14521 ttgatcgcgg ttccggtgct ggcggtgctg gggctgttgg cgggctttgc gctcagcctg
    14581 tcggcggcct cgcgcgcgtt cgagcatggg ttgatcgccc agcggcaggc ggccctggtg
    14641 gaagccattg ccgccgatgc cgaccggctg gatcaggcca gcctgcagcg ccggctggcc
    14701 gcctatcggg cgctggtggt ggaggagcgg ccctttcttt ccgccgctga tgccgccgcg
    14761 caaggggccg aacagcgccg cgccgctgcc ctttcggcgc tggccggcgt ggcggcagac
    14821 cggccgcgga tggccgcgct ggtggccgcc attgccgcag aggaagcgca tgaggtagcc
    14881 gctacgcgcg ccggccttgc ccgcttgcgg cgcaacatgg tggtgctggg cgcattgctg
    14941 gcggcagcgg cgctgggcgc ggtggcggca ggggccttgc agatgctgcg ggccaaccgc
    15001 gatctggccg gccaggtggc cgagcgcacc gccgcgctgg aggcggtgga tgcctcgcgc
    15061 cggttgttct tcgccaaggc cagccatgaa ttgcgcacgc ccgtgaccgc catccgcacc
    15121 attgctgaag tggcgcagga aggcgccgac caggcgcagg cgctggccga tatcgtggcg
    15181 caggcgggct ttctggaaca tcgcattgcc gatctgatgg cgctggccgc cgccggcgag
    15241 gggcagccgg cgctggtgct ggcgccgggc gatctggccg gggtgatcgc cggggcggcg
    15301 gcgcaggccg ggccctatgc ccgctcgctg ggggtggaga tcgtggtgac ggcgcccgcc
    15361 gcccccctca tcgcccggat cgatggcgac aggctggcgc aggcgctgct ggcggtgatc
    15421 gacaatggcc tcaaattttc cgatcccggc gcccggctgg aactggcgct ggaggcggcc
    15481 agcgggcagg cggtgctggc cgtggcggat agcgggcccg gcgtgctgcc gcccgaactg
    15541 ccgcgcattt tcgatgccta ttaccaggcc ggcgccggcc gtgcgcgcgg gggcagcggg
    15601 ctggggctgg cgcttgcccg ctgggtggtg gagcgccatg gcggcactat cctggcggaa
    15661 aaccgcgtgg gcggtggctg ccgcatcgtg atcggcctgc cgctggtggc agaaggggag
    15721 gggcaatgat cctgctggtg gaagatgatg tggcgatcgg ccgctcgctg gcgcaggggc
    15781 tggcggcgcg cgggtttgcc gtgcgctggc tgcggcaggc ggcgcaggcg gccggcgcgc
    15841 tcgataccgg cgaggtggcg gtggcggtgc tcgatcttgg cctgcccgat ggcgacgggc
    15901 tggccctgtg ccgccagtgg cgcgcggccg gccaccgcct gccggtgctg atgctgaccg
    15961 cgcgcgccgc gctggacgac cggctggaag gctttgccgc cggcgccgat gattacctgc
    16021 ccaagccttt cgcctttgcc gagctggccg cgcgggtgga agtgctggcc cgccgcgccg
    16081 cccagctgcc ggcagcgcct gtgctgtggg gccccttcgc gttcgacagc gccgccggcc
    16141 aggtgctgcg cgatggcgcg ccgctggctt tcgagcccaa ggcgctggcg ctgctgctgc
    16201 ggctggccca ggcgcgcggc ggggtggtgg cgcgcgatgc gctgatcgat gccggctggg
    16261 gcgaaatggc cgccgttggg ggcaacacgc tggatgtggc gatcagcgcg ctgcgccgcc
    16321 ggctggccga tgtggcgcct gagctggcgg tgaaggcagt gaaggggcag ggctatcagc
    16381 tcgaccttat accttaggtc agcctgagga aaagctcagg atcgcgcgcc gccaacccgg
    16441 taagcctttg tgcagaacac aaggtttcat gggctgagga tggaaatgca caacaagggg
    16501 atgcggctgc gcggtgccag cctggcggtt atggcggtgc tggccggcac acaggcacgg
    16561 ggcgcagagg caaacgctgc caatggcgcg aacggggatc cgatcatcgt ggtgacggcg
    16621 ccgatcgcca ccgacacggc ccggctggat gatgtgcccg ccaatgcgca ggtgctggcc
    16681 ggcgatgcgc tgggccggca gaaccatgcc aatctggccg atctgctcaa tgccaatctc
    16741 ggctcggtca gcctctccaa cggttcgggc agcccctatc agagcgatgt cgcctatcgc
    16801 ggctttcagg ccacctcgct gctgggctcg cccacgggcc tgtcggtgtg gctggatggg
    16861 gtgcggatga acgaggcctt cggcgccatc gtcaactggg atctgatccc gatgaacgcg
    16921 gtgagcgcgg tggaagtgct gcccggctcc aacccggtgt tcggcctcaa cacgctgggc
    16981 ggcgcgctgg tgctgacgac gaagaacggg gcggataatg gcggcctctc gatcatggcc
    17041 gagggcggct cgttcaaccg gcgcagcgtg gcggcagagg cgggcggcac cgcggcgaac
    17101 aaggcgatcg actggttcct ggccggcaat tacgacgagc aggacggcta tcgcctgcac
    17161 acccacacgc gggtggagca gctttatggc aagctgcgct ggcatggcgc gggcgccaat
    17221 gccgagctgg gggcgatgtg gtcggccagc cagctcaatg gcacgcaaag cctgccgctt
    17281 tccatgctct ccacccctga ggcgtcctat acctggcccg attcggtggc caatcaccag
    17341 ctgctgatca acctcaaggc cgatgccagc ctggcggccg ggctgaagat cagcggcaat
    17401 gtctatttcc gccgctccaa cgcgtccggc gtcaacagca atgccagcct cgacgacaat
    17461 tgcggcgaga gccgcgattg cagcgccagc gcccccggcg gcaccgcggt ggatctctac
    17521 cagaccaacc cctatctggc cggcagcgcc cgcgccaatg gctttgtggc ctatgccggc
    17581 accctgccga tccacgacta taccgacaac atcaacacct cgctggtctt ctccagcacc
    17641 cgccagcaca gctggggcgg caacctgctg ctggacagcg acgccccgct gctgggccgc
    17701 aagaacgatc tcaatctggg cggcagcttc gagatcgccg acatcaccta cgaccagagc
    17761 acctatctgg cctatctggt gggctatcag acggtgcaga tgccgtggaa tttcaaatat
    17821 ggcagcgctg gtggtttcga gggcagcccg cttgtcaaca gcgtcgatgt ggcctcgcac
    17881 agcgccagct tcaacctctt tgtgcgtgac acgctgaagc tgaccgagac gctgggcctg
    17941 accgcctcgc tcagctacac cagcacccat gtcagcctgg gcggcaccaa cagcaccttc
    18001 ctcaacgagg atggcggctt cagctggacc ggcgccgacg ggcagaaata ttacaacccc
    18061 gcctatatcg gcgccagcta ctggacaacg ccttcctcgg gcagttcgcg gctggccacg
    18121 gccagcattc ccacaggcgg cgtggccggg cccgaagtgg tggcggtaac aggcgcccat
    18181 ctctaccacc gcttcaaccc ggcggtgggg ctggcctgga acccgctgcc ggcgctgggc
    18241 ctgtttgcca gctattccga ggcgatgcgc gcgcccaccg ccatcgagct ggcctgcgca
    18301 gaccctgcca ccccttgcgc cctgcccacc ggcttcaacg gcgatcccga tctgaaggcg
    18361 gtggtggcgc gcacggtgga agtgggcgcg cgcggccggg tgggcggcca tgtcagctgg
    18421 aacgcggcgc tctatcgcac cgggctgacg aacgacatcc agttcatcta cgacacgtct
    18481 ggccttggct atttcgccaa tgtcggccgc acggagcgcc gcgggctgga gctgggcctg
    18541 gcggcagact gggcgcggct gcacctgtcg gccagctatg gccatgtggt ggcgacatat
    18601 cgctccacct tcaccgatgc cagcggcgat acggtgcagg ccggcaacca catccccggc
    18661 atcccggccg acagtttcaa gctgcgcgcc gtttatgcgc cgaccaaggc gctggcgctg
    18721 ggggtcaatc tcatcgccgt ctccagccag tgggcccatg gggatgaggc gaacctttat
    18781 ggcgcggtgc ccggctatgc gctggtgaat gccgatctgc atgtagtgcc gcgccccggc
    18841 tgggagctgt ttgccaacat caccaacctc ttcaaccgcc actatgccac ctatggcgtg
    18901 atgggcacca acatctacac cggcaatgac gagcagttcc gcacgccggc acagggccgg
    18961 gcgtttgtgg ctgg
//
LOCUS       DASRYE010000301        16662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_105947,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000301 DASRYE010000000
VERSION     DASRYE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105947"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFF98_15490"
     CDS             <1..324
                     /locus_tag="VFF98_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZU65089.1"
                     /translation="QPAAVPSPPPHRDVPANPPPKPAPPAQNAAQKTGEKPAQGTLVV
                     QVGAFSTEAHARAAARPISGSVSASGKFWRVRMGPFATRGEAEAALGKARAAGYSEAR
                     IQRVD"
     gene            408..1655
                     /locus_tag="VFF98_15495"
     CDS             408..1655
                     /locus_tag="VFF98_15495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZU65090.1"
                     /translation="MFGAVPVAALASLAISHASNHAVPAASPPAAAPSPVPAAPVAIA
                     VDGTALPPPHEVEAAPIAMLVDLNSGRTLYAKNEAEAFPPASVTKTMTAFVAFELLAE
                     GRLKADQHFTVSDAAWKEWHAKGSRMFIPYRASVSVDDLLMGIMNVSANDGCIVLAEG
                     VAGSVPGWVAMMNDAARRLGMSGSHFGTPNGWMDKGVTHYTARDLVKLADAMITRYPA
                     YFRRYVGHAEFSWNGITQRNHDPILGVVPGADGIKTGFTGEAHYTYLGTAERGGRRLV
                     MVLAGVPTPREREKAAKALMEWGFSAWDGAPLVAAGKVIARAEVQGGTAPAVDLVAPR
                     AYAASLPHGDHAPVSMRVIYTGPLQAPIARGAQVAQLAIRVGAGPEAHLPLVAGADVP
                     AGSAVDRLAAGFARLWPGSGKRS"
     gene            1652..2302
                     /gene="tmk"
                     /locus_tag="VFF98_15500"
     CDS             1652..2302
                     /gene="tmk"
                     /locus_tag="VFF98_15500"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZU65091.1"
                     /translation="MTAGGLFIALEGGEGAGKSTQGRLLAEALRARGAEVVLTREPGG
                     TAGAEAIRALLLDAGGAGWGARAEALLFAAARADHVARLIRPVLARGGVVICDRFLDS
                     SRAYQGGGGGLEDADIRALHAIGSEGLLPDITLLVEVPPAVAAARLAGRAAATAAGPD
                     RIEQQGEAYHARVAAAFARFAAEEPARFARIDGTGDAQAVHARVLAALAPLLGAAA"
     gene            2299..3267
                     /locus_tag="VFF98_15505"
     CDS             2299..3267
                     /locus_tag="VFF98_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZU65092.1"
                     /translation="MSELAWQGHEAAWREWRAALASPRLHHGWLLAGPQGLGKGHFAR
                     VAAAALVAEEGVPQPPLEAHPDILIPEHPPATREDEKKREEGLPYAKKRAIPVDEIRR
                     LQHRLITRPTLGSRRVVILDPADDLETAAANALLKSLEEPPAGTYFLLVTHRPGRLLP
                     TVRSRCRVLRFAPLGDAAVAAILAGAVPGADGATRAAAVAAAKGSPGAALSYVESGLG
                     AAHGLMRAMLAEGDADLSRRGALAQELGARPAREVMQATLDLARAVLAGALRDAPKPA
                     QARIIDAHGALVRLAAEAPTHNYDPGLLAMAIGALLAGCAMPKTAR"
     gene            3297..4856
                     /gene="metG"
                     /locus_tag="VFF98_15510"
     CDS             3297..4856
                     /gene="metG"
                     /locus_tag="VFF98_15510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZU65093.1"
                     /translation="MAEPFYITTAIAYPNGRPHIGHAYEAIAADVIARFQRAAGRDVR
                     FQTGTDEHGLKMAQKARELGITPRALCDEMSGHFRAMCDALNISYDVFQRTTDPAHYA
                     ASQALWQRMAAKGDLELGRYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVEWTVE
                     ESWFFRLDRYQQKLLDLYAAHPEFIQPESRRNEVMRFVEGGLNRLSVSRTSFDWGVPV
                     PDAPGHVMYVWVDALTNYLTGLGFPAETAEMGKWWPADIHLIGKDIVRFHTVYWPAFL
                     MSADLPLPRQVFGHGFLLHRGEKMSKSLGNVVDPLELAERFGVDALRYFLMREVSFGN
                     DGSYSAEAIVTRCNAELANSFGNLAQRSLSMVFKNLAGALQAGLPETEADAILRATVG
                     SEIAEMRARFAALDFSAGLDAWMRAVFACNAYVDEQAPWALRKTDPARMAAVLMVLLQ
                     VVRDLAIAVRPVVPTAIDRLLDQLGVAADARDYAALDQAGWFADLAASGFAISQPSGV
                     FPRLELPADGE"
     gene            4853..5632
                     /locus_tag="VFF98_15515"
     CDS             4853..5632
                     /locus_tag="VFF98_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZU65094.1"
                     /translation="MTLIDSHCHLNYKGLVESQDEVLARARAAGISGFLNISTRQREW
                     AEVIATAERHQDVWATVGIHPHEADEHADLGEGALLEATHHPRVIGIGETGLDYYYDH
                     SDRAVQQALFRTHIAVARQTGLPLIIHTRDAEEDTARILAEETQVGAFPALIHCFTAS
                     ADFAEKVLALGLYISLSGIVTFRNAAALQEVARTVPAERLLVETDSPFLAPVPHRGKV
                     CEPAFTADTARFVADLRGVGVAELGALTSANFFRLFAKAAA"
     gene            5629..6408
                     /locus_tag="VFF98_15520"
     CDS             5629..6408
                     /locus_tag="VFF98_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU65095.1"
                     /translation="MKVVLLGSGTSTGVPRVGGEHGADWGDCDPAEPRNRRTRASLLV
                     ESEAGSRLLVDTSTDLRQQLLANDIHRLDAVFWTHDHADHCHGIDDLRPLRYGRGGPM
                     AGYGSEETVRRLRARFGYVFAGQHGYHTIVQIDTLDRLRMCAGFGVGWCQMPHGPAQS
                     TGYRFETDGKALGYATDFSAITAEMVALFVGVDLLVVDCLRREPHPTHAHLAMSLELA
                     QACGAGRAVLMHLDKSMDYRTLSEEVPAHVTVGYDGMVVTV"
     gene            6484..7275
                     /gene="mazG"
                     /locus_tag="VFF98_15525"
     CDS             6484..7275
                     /gene="mazG"
                     /locus_tag="VFF98_15525"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZU65096.1"
                     /translation="MTDIQFPAQAALARLLAIMARLRDPVAGCEWDVAQNFATIAPYT
                     IEEAYEVAEAIAQDDMPSLREELGDLLLQVVFHARMAEEAGHFAFAEVADAISDKMVA
                     RHPHVFAEEAAGARSREARWEALKAEERAAKGAASALDGVALALPALMRAEKLQKRAA
                     RVGFDWPDTQGPAAKVVEEMAELAEAQGDAHRAEEAGDLLFAAVNLVRAHGVPAEEAL
                     RAANAKFERRFRAMEALAEAEGRDFAALSLEAQEALWQVVKTREG"
     gene            complement(7289..8590)
                     /gene="hflX"
                     /locus_tag="VFF98_15530"
     CDS             complement(7289..8590)
                     /gene="hflX"
                     /locus_tag="VFF98_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZU65097.1"
                     /translation="MARGARALIICPDFHRRPAATDLAARLEEARGLALAIGLVIADA
                     IAMPIREARPGTLFGEGQIENIGIQCNLHEADLIIVDGALSAVQQRNLEEKLKRKVID
                     RTGLILEIFGERAATAEGRLQVELAHLDYQAGRLVRSWTHLERQRGGFGFLGGPGETQ
                     IEADRRMIRDRMARIRRELEQVRRTRGLHRERRGKAPWPVLALVGYTNAGKSTLFNRL
                     TGAEVMAQDLLFATLDPTMRAIRLPGVDKAILSDTVGFISDLPTQLVAAFRATLEEVT
                     AADIVIHVRDIANPDTLAQKEQVLSVLEDLGLLEEGTRAPSVPIIEVWNKWDLLEPDH
                     AADLLRQAQGESADAEAIVPLSALTGAGCDDLLAAANRLLTGDAREHRIVLPVGEGAR
                     IAWLYAHGEVLTDEVTELDGEPAHALCVRLTPREFGRFSRI"
     gene            complement(8617..9312)
                     /gene="hfq"
                     /locus_tag="VFF98_15535"
     CDS             complement(8617..9312)
                     /gene="hfq"
                     /locus_tag="VFF98_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZU65098.1"
                     /translation="MTGRTLSARPKLKPAAEPLAEEAEAATEATETLAADAEPAPAPR
                     ARAPRSSTKADAVPADTIAAPASAAKGSQNLQDQFLNHLRKNKTPVTMFLVKGVKLQG
                     IVTWFDNFSILLRRDGQSQLVYKHAVSTIMPSLPIDTRLFGNGAEGAKKARLLQDVFL
                     SSIRAAAVQVTMFLVNGVMLQGRVAAYDLFCMLLEREGYVQLAYKHAVSTIQPVTPVD
                     LTGDWDGEEDASA"
     gene            complement(9454..10845)
                     /locus_tag="VFF98_15540"
     CDS             complement(9454..10845)
                     /locus_tag="VFF98_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU65099.1"
                     /translation="MALDILIVDDERDIRELVAGVLSDEGYDTRTAGDSTAALEAIDQ
                     RRPSLVLLDVWLHGSPMDGLEVLDQIKQREPDLPVIIFSGHGNIDTAVAAIGRGAVDF
                     IEKPFETERLLHLVGRATETERLRRENARLRHDTISSQEFTGSSSAINHVRATLKRVA
                     NTGSRLLISGPSGVGKEVAARLLHSWSPRAGNAFVSVNSARITPERFEEELFGEEQDG
                     QLIRAGLLEMADGGTLYLDEVGDMPLSTQARILRVLTEQSFVRVGGHRQIRVDVRVVS
                     STSRNLEKEIAERRFREDLFYRLNVVPVSVPPLAERRDDIPALVAHFFARYANDQGLP
                     PPEIGMEAMAALQAYEWPGNVRQLRNVVERTVILAPRERLGRIEPDMLPGEIVHGRLP
                     GDSGMSALMGVPLREARENFEREYLRVQIRRFSGNISKTASFIGMERSALHRKLKLLG
                     MVDRREDEEGEEG"
     gene            complement(10883..13165)
                     /locus_tag="VFF98_15545"
     CDS             complement(10883..13165)
                     /locus_tag="VFF98_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU65100.1"
                     /translation="MNTAPPLVPRRPPRWWRRLQLMLRRDGIFRWLEVISVVAFLAAT
                     LMSWRALADQDARGELLPSGLTATLLVGTLVPGMAILMLIGRHMALNRARARFGGKGR
                     LHVQLVFLFSVIAALPTLLVAIFASWLFQSGVEFWFSDNSRGLLENANKLARGYYEQT
                     QRDVSDNTVAMAYDVRDALSRVSLVSPEFREGFFYQVVQRKLDDVAILQKGPNGQLNT
                     AAYVDPSGRPRDWVTEAELRRIDAGEPIIVNATADRIEAMTPIDRRAGIYLYAARKSD
                     LLAISQGKRADNIVRAYQVLTQRARLLQLRFNVALFIASLALVGLAVWFGLRFADKQV
                     QPLYDLAIAARQVGSGNYTLRVGGRVGFDEIGLVTRAFNRMASQIERQTAAVLRANQQ
                     LDERRAFIEAVLESVTAGIISLDEEGAIRLMNSSAQKLLLDPQEAQLAPPIGHPLVEL
                     APQLAALAGHEGSGGQGRAHVSEAIVQHGKGGELLTLAVKVTDNRGGHVITFEDITRQ
                     LLDQRQAAWSDVARRIAHEIKNPLTPIQLATERLSRRYRKQILLDPELFEELTATIIR
                     QVGDLRKMVDEFSSFARLPKPVFRGEDPADLARQALFLQEVARPDISFSFAALGEAGL
                     IACDRHQFGQAMTNVLKNATEAVEARAKSADADYRGRIRLELSGTADSVLVMVIDNGI
                     GLPQDRERIVEPYVTTREKGTGLGLAIVKKIVEEHGGEMIFASAPDGGTQVSLRFARD
                     PLAKPAPGAADALNDAQAAQ"
     gene            complement(13240..14658)
                     /locus_tag="VFF98_15550"
     CDS             complement(13240..14658)
                     /locus_tag="VFF98_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZU65101.1"
                     /translation="MTVLLVEDDESIAIVITAALEAEGYEVARCDSVAERDRLLSLHA
                     YAALVTDVMLTDGDGLETLALARSLRPHMPVIVLSAQNTLDTAVRATDGGAFEYFPKP
                     FDIDELVRTVRQATGSAGRAPGEVEADTEAQGLPLVGRSASMQAVYRMITRVLKNDLT
                     VLILGESGTGKELVAEAIHKLGHRREGPFVAVNTAAIPAELIESELFGHEKGAFTGAV
                     ARHIGKFEQAAGGTLFLDEIGDMPMQAQTRLLRALQSGSIRRVGGREEVRIDARIIAA
                     TNKDLVPMIAAGQFREDLYYRINVVPINLPPLRARADDIEALARHFLALAAQEGLPRR
                     HLSADAARLLQRQPWHGNVRELRNFIYRVALLAREDLVDEASLLPMLEQNARAEAPAP
                     GPVGLEEAVAHWLDETRPPQGLVYDTALAAFERPLFTGILRQTGGNQLRAAQVLGINR
                     NTLRKRLNDLAIDPDSFLRRDG"
     gene            complement(14675..15802)
                     /locus_tag="VFF98_15555"
     CDS             complement(14675..15802)
                     /locus_tag="VFF98_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU65102.1"
                     /translation="MSEAAAPPARARAELPDARRQLACLPLAVITLGPDLRIAAANPA
                     AEQFLSQSFRRLQGRALGDVLSFGEPLLTERLHRADAPLSARQIDVVVKGKDSRRLDV
                     TVAPIADAEGWQVLTLHDSAATEALGENTVGSEEGMVRAPEILAHEIKNPLAGIKGAA
                     QLLARKVDGHDRALTTLIAEEVDRIAKLIDQMQTLSRRTVEPVEPCNLHEVIRRASAI
                     LTASGRAPRIEEEFDPSLPPVLGNAHALTQVLINLLANGRDACADQPDPRLVVRTRFV
                     SGLQLLATGGRQAVRLPIEVRISDNGPGIAPHMRDHIFEPFVSTKKSGQGLGLALVRK
                     LVRDMNGRITHDRDERGGWTHFRLHLPVAPGRSRKQAQLAG"
     gene            complement(15799..>16662)
                     /gene="dusB"
                     /locus_tag="VFF98_15560"
     CDS             complement(15799..>16662)
                     /gene="dusB"
                     /locus_tag="VFF98_15560"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZU65103.1"
                     /translation="EMIASPAAIRETRQSIQKAAWDPAEEPVSMQLVGCEPEQMAEAA
                     KLSEDKGAAIIDINMGCPVRKVVNGEAGSALMRDLPLAARLIAATVKAVKVPVTVKMR
                     MGWCHDSLNAPELARIAEDLGARMITVHGRTRNQMYKGTADWAFVRRVKEAVQVPVIV
                     NGDICNVADVAQAMAQSGADGVMIGRGAYGRPWLLGQVMHWLKTGVLLDDPTLDEQFA
                     TMMEHYEDILALYDIRAGVNLSRKHLGWYIKGIPGAADLRNKVNFMDDPAEVKATLAE
                     FYRPFLKARAA"
BASE COUNT         2589 a         5558 c         5733 g         2782 t
ORIGIN      
        1 cagcctgccg cagtgccttc cccgccgccg catcgcgacg tgccggccaa cccgccgccc
       61 aagccggccc cgcctgcgca gaatgccgcg cagaaaaccg gggaaaagcc cgcgcagggc
      121 acgctggtgg tgcaagtggg cgccttttcc accgaagccc atgccagggc cgctgcccgg
      181 ccgatttccg gcagtgtcag cgcctcgggc aagttctggc gggtgcgcat ggggccgttt
      241 gccacgcgcg gcgaggccga ggcggctctg ggcaaggccc gggcggcggg gtatagcgaa
      301 gctcgaatcc agcgcgtcga ttgagcctgc gggcgccccg ccggtgcgcg cagggggcaa
      361 ggctggcaaa tggtgtgcgg ttgaaacaga gcttcaaggc gaaaatgatg tttggcgcgg
      421 tgccggtggc ggcgctggcc agcctggcga tcagccacgc cagcaaccat gccgtgcccg
      481 ctgccagccc gccggccgct gccccttcgc cagttcctgc ggcgcctgtg gccattgccg
      541 tcgacggcac cgcgctgccc cctccacatg aggtggaggc agcgccgatt gccatgctgg
      601 tcgatctcaa ttccggccgc acgctttatg ccaagaacga ggccgaggcg ttcccgccgg
      661 cctctgtcac caagacgatg acggcctttg tcgcctttga actgctggcc gaggggcggc
      721 tgaaggcaga ccagcatttc accgtgtctg acgcggcgtg gaaggaatgg cacgccaagg
      781 gcagccgcat gttcattccc tatcgcgcca gcgtgtcggt ggatgatctg ttgatgggca
      841 tcatgaatgt ctcggccaat gatggctgca tcgtgctggc cgagggcgtg gcgggcagcg
      901 tgccgggctg ggtggcgatg atgaacgatg ccgcccggcg cctgggcatg agcggcagcc
      961 atttcggcac tcccaacggc tggatggaca agggcgtgac gcattacacc gcgcgcgatc
     1021 tcgtcaagct ggccgatgcg atgatcacgc gctatccggc ctatttccgc cgctacgtgg
     1081 gccatgccga attcagctgg aacggtatta cccagcgcaa ccacgatccc attctgggcg
     1141 tggtgccggg cgctgatggc atcaagacag gtttcaccgg cgaggcgcat tacacctatc
     1201 tcggcacggc cgagcggggc gggcggcggc tggtgatggt gctggccggc gtgcccactc
     1261 cgcgagagcg ggagaaggcc gccaaggcgc tgatggaatg gggcttttcc gcctgggatg
     1321 gggcgccgct ggtggctgct ggcaaggtga ttgcgcgggc cgaggtgcag gggggcacgg
     1381 cgccggccgt cgatctggtg gcgccgcggg cctatgccgc cagcctgccg catggcgatc
     1441 atgcgccggt gtcgatgcgg gtgatctaca cagggccgct gcaggcgccg atcgccaggg
     1501 gcgcgcaggt ggcgcagctg gccatccgcg tgggcgctgg gccagaggcg catctgccgc
     1561 tggtggccgg tgccgatgtg ccggcgggca gcgcggtgga ccggcttgcg gccggctttg
     1621 cgcggttgtg gccgggatcg ggcaagcgct cgtgacggcg ggcgggctgt tcatcgcgct
     1681 ggaagggggc gagggggcgg gcaaatccac ccaggggcgc ttgctggccg aggccctgcg
     1741 cgcgcgcggc gccgaagtgg tgttgacgcg tgagcccggc ggcactgccg gcgctgaggc
     1801 gatccgcgcg ctgctgctgg atgcgggcgg cgccggctgg ggcgccaggg ccgaggcgct
     1861 gctgtttgcc gcagcgcgcg ccgatcatgt ggcgcggctg atccgcccgg tgctggcgcg
     1921 cggcggcgtg gtcatttgcg acaggtttct cgattcgagc cgggcctatc agggcggcgg
     1981 cggcgggctg gaggatgccg acatccgcgc gctgcatgcg attggcagcg aggggctgct
     2041 gcccgatatc accctgctgg tggaagtgcc gccggcggtg gcggcagcgc gcctggccgg
     2101 gcgggccgcc gccactgctg ccgggccaga ccggatcgag cagcagggcg aggcctatca
     2161 cgcccgcgtg gccgccgctt tcgcccgctt tgcggcagaa gagcccgccc gctttgcccg
     2221 gatcgatggc acgggcgatg cgcaggcggt gcatgcccgg gtgctggcgg cgctggcgcc
     2281 tttgctgggc gctgcggcat gagcgagctg gcctggcagg ggcatgaagc ggcctggcgc
     2341 gaatggcgcg cggcgctggc ctcgccccgg ctgcatcatg gctggctgct ggccgggccc
     2401 caggggctgg gcaagggcca tttcgcccgc gtggccgccg ccgcgctggt ggctgaggaa
     2461 ggcgtgcccc agccgccgct ggaagcccac cccgatatcc tcatccccga acacccgcca
     2521 gccacgcgcg aggatgagaa gaagcgcgag gagggcctgc cctatgccaa gaagcgcgcc
     2581 attccggtgg acgagatccg ccgcctgcaa caccggctga ttacccggcc aacgctgggc
     2641 agccgccggg tggtgatcct cgatccggct gacgatctgg agacggcggc ggccaacgcc
     2701 ttgctgaaaa gcctggagga gccgcccgcc ggcacctatt tcctgctggt gacgcatcgg
     2761 cccgggcggc tgttgcccac ggtgcgttcg cgctgccggg tgctgcgttt tgccccgctt
     2821 ggcgatgcgg cggtggcggc cattctggcc ggcgcggtgc cgggggccga tggcgccacg
     2881 cgggcggcgg ccgtggcggc ggcgaaggga tcgccgggcg cggcgctgtc ctatgtggag
     2941 agcgggctgg gggcggccca tgggctgatg cgcgccatgc tggccgaggg cgatgccgat
     3001 ctgtcgcgcc ggggggcgct ggcgcaggaa ttgggcgcgc ggccggcccg cgaggtgatg
     3061 caggcaacgc tggacctggc ccgcgcggtg ctggccgggg ccctgcgcga tgcgccaaag
     3121 cccgcccagg cccgcatcat cgatgcccat ggcgcgctgg tgcgcctggc cgccgaggcg
     3181 ccgacgcata attacgatcc gggccttctc gccatggcaa ttggcgcctt gctggcaggc
     3241 tgcgccatgc ctaagacggc tcgctgacag tttcccgcaa aggtttaagg ggccccatgg
     3301 ccgagccatt ctatattacc accgccatcg cctatcccaa cgggcgcccg cacatcggcc
     3361 acgcctatga ggcgattgcc gccgatgtga tcgcccgctt ccagcgcgct gcgggccgcg
     3421 acgtgcgttt ccagaccggc actgacgagc atgggctgaa aatggcccag aaggcgcgcg
     3481 agctgggcat caccccgcgc gccctgtgcg acgagatgtc gggccatttc cgcgccatgt
     3541 gcgatgcgct gaacatcagc tacgatgtct tccagcgcac taccgacccg gcccattatg
     3601 ccgccagcca ggcgctgtgg cagcgcatgg cggccaaggg cgatctggaa ctgggccgct
     3661 atgaaggctg gtattcggtg cgcgacgagg cgttctacga cgagagtgag ctggtggcgg
     3721 gcgagggcgg cgagaagctt tccccccagg gcacgccggt ggaatggacg gtggaggaaa
     3781 gctggttctt ccggctcgat cgctatcagc aaaagctgct cgatctttat gccgcccacc
     3841 ccgaattcat ccagccggaa agccgccgca acgaggtgat gcgctttgtg gaaggcgggc
     3901 tgaaccggct gtcggtcagc cgcaccagct tcgattgggg cgtgccggtg cccgatgcgc
     3961 cgggccatgt catgtatgtg tgggtggatg cgctgaccaa ttatctgacc gggcttggct
     4021 ttccggccga aacggcggag atgggcaagt ggtggccggc cgatatccac ctgatcggca
     4081 aggatattgt gcgcttccac acggtctatt ggccggcctt cctgatgagc gcggatctgc
     4141 ccctgccgcg ccaggtgttt ggccatggct tcctgctgca ccgcggcgag aagatgtcga
     4201 agagcctggg caatgtcgtc gatccgctgg agctggccga gcggttcggg gtggatgcct
     4261 tgcgctattt cctcatgcgc gaggtcagct tcggcaatga tggcagctat tcggccgagg
     4321 cgatcgttac gcgctgcaat gccgaactgg ccaacagttt tggcaatctg gcgcagcgca
     4381 gcctgtcgat ggtgttcaag aatttggccg gcgcgctgca ggcgggcctg cctgaaaccg
     4441 aggccgatgc catcttgcgg gccaccgtgg gcagcgagat cgccgagatg cgcgcccgct
     4501 ttgccgcgct cgatttttct gccgggctgg atgcgtggat gcgtgcggtg ttcgcctgca
     4561 acgcctatgt ggacgagcag gcgccctggg ccctgcgcaa gaccgacccg gcgcgcatgg
     4621 cggcggtgct gatggtgctg ttgcaggtgg tgcgcgatct ggcgattgcc gtgcggccgg
     4681 tggtgcccac cgcgatcgac cgcctgcttg atcagctggg cgtggcggcc gatgcgcgcg
     4741 attatgccgc gctcgatcag gccggctggt ttgccgatct ggccgccagc ggctttgcca
     4801 tcagccagcc cagcggcgtg ttcccgcgcc tcgaactgcc ggcggacggc gaatgacgct
     4861 gatcgattcc cactgccacc tcaattacaa ggggctggtg gaaagccagg acgaggtgct
     4921 ggcgcgcgcg cgcgcagccg gcatttcggg ctttctcaac atctccaccc gccagcgcga
     4981 atgggccgag gtgatcgcca cggccgagcg ccatcaggat gtgtgggcca cggtgggcat
     5041 ccatccgcat gaggccgacg agcatgccga tctgggcgag ggggccctgc tggaggcgac
     5101 ccatcacccc cgggtgatcg gcattggcga gaccgggctg gattattact acgaccattc
     5161 cgaccgggcg gtgcagcagg cgttgttccg cacccatatc gccgtggcgc ggcagactgg
     5221 cctgccgctg atcatccata cccgcgatgc cgaggaagac accgcgcgca ttctggcgga
     5281 agagacgcag gtgggggctt tccccgcgct gatccactgt ttcaccgctt cagccgattt
     5341 tgccgaaaag gtgctggcgc taggcctcta tatctcgctg tcgggcatcg tcacgttcag
     5401 gaatgcggct gccctgcagg aggtcgcccg caccgtgccg gcagaacggc tgctggtgga
     5461 gacggacagc ccgtttctgg cccccgtgcc ccatcgcggc aaggtgtgcg agccggcctt
     5521 cacggccgat acggcgcgct ttgtggcgga tttgcgcggg gtgggcgtgg cagagctggg
     5581 cgcgttgacc agcgccaatt tcttccgcct gtttgcaaag gcggcggcat gaaggtggtg
     5641 cttctgggca gcggcacctc taccggcgtg ccgcgcgtgg gcggcgaaca tggcgccgat
     5701 tggggcgatt gcgatccggc cgagccgcgc aaccggcgca cccgcgcctc gctgctggtg
     5761 gaaagcgagg cgggcagccg gctgctggtg gacaccagca cggacctacg ccagcaattg
     5821 ctggccaacg atatccaccg gctcgacgcg gtgttctgga cgcatgacca tgccgaccat
     5881 tgccacggca tcgatgatct gcgcccgctg cgttatggcc gcggcggccc gatggccggc
     5941 tatggcagcg aggagacggt gcggcggctg cgggcgcgct ttggctatgt gtttgccggc
     6001 cagcacggct atcacacgat cgtgcagatc gacacgctgg accggttgcg catgtgcgcc
     6061 ggctttggtg tgggctggtg ccagatgccg catgggccgg cgcaaagcac tggctatcgc
     6121 tttgagacag acggcaaggc gctgggctat gccacggatt tcagcgcgat cacggccgag
     6181 atggtggcgc tgtttgtcgg ggtggatctg ctggtggtgg attgcctgcg ccgcgaaccg
     6241 catccgaccc atgcccatct ggcgatgtcg ctggagctgg cgcaggcctg cggtgctggc
     6301 cgcgcggtgc tgatgcatct cgacaagagc atggattatc gcacgctgag cgaggaagtg
     6361 ccggcgcatg tgacagtggg ctatgatggc atggtggtga ctgtctgatc ctcataccct
     6421 gatccacacg ccgccctgca tttaacataa taaatattat cgaacttaca ttggaggttc
     6481 gccatgacgg atattcagtt ccccgcacag gccgctttgg cccgtctgct ggcgatcatg
     6541 gcgcggctgc gtgatccggt ggcgggctgc gaatgggatg tggcgcaaaa tttcgccacc
     6601 atcgcgccct acacgatcga ggaagcctat gaggtggccg aagccatcgc gcaggacgac
     6661 atgccgagcc tgcgcgagga actgggcgac ctgctgctgc aggtggtgtt ccatgcccgc
     6721 atggccgagg aagccggcca cttcgccttt gccgaggtcg ccgatgcgat cagcgacaag
     6781 atggtggcgc gccaccccca tgtgtttgcc gaggaagctg ccggcgcgcg cagccgcgag
     6841 gcccgctggg aagcgctgaa ggcggaggaa cgcgccgcca agggcgccgc cagcgcgctg
     6901 gatggcgtgg cgctggcgct gccggcactg atgcgggcgg aaaagctgca gaagcgcgcc
     6961 gcgcgcgtcg gttttgactg gcccgatacg caaggccccg ccgccaaagt ggtcgaggaa
     7021 atggccgagc tggcagaagc gcaaggcgat gcccaccgcg ccgaggaagc cggcgacctg
     7081 ctgtttgccg cggtgaacct ggtgcgcgcc catggcgtgc cggcggaaga ggcgctgcgg
     7141 gccgccaacg ccaagttcga gcgccgcttc cgcgcgatgg aagcgctggc cgaggcggaa
     7201 gggcgcgatt tcgccgccct gtcgctggag gcgcaggaag ccctatggca ggtggtgaaa
     7261 acgcgcgagg ggtaatccct ccgcgcgttt aaatccttga aaagcgacca aactcgcgcg
     7321 gtgtcaaccg cacgcacagg gcatgggcag gctcgccgtc cagctcggtc acttcatctg
     7381 tcagcacttc gccatgggca taaagccagg cgatccgagc cccctcgccc accggcagca
     7441 caatgcggtg ctcgcgcgcg tcgccggtca acaggcggtt ggctgcggcc aagagatcgt
     7501 cacaccccgc cccggtcaac gccgagagcg gcacgattgc ctcggcatcg gcggattccc
     7561 cctgcgcctg gcgcagcagg tctgcggcgt gatccggctc cagcagatcc catttgttcc
     7621 acacctcgat gatcggcacc gagggcgcgc gcgtgccttc ttccagcagg cccaggtctt
     7681 ccagcacgga aagcacctgt tccttctggg ccagcgtgtc cgggttggcg atatcgcgca
     7741 catggatgac gatatcggcc gccgtcactt cctccagcgt ggcgcggaaa gcggccacca
     7801 gctgcgtcgg caggtcggag atgaagccca ccgtgtcgga gaggatcgcc ttgtccaccc
     7861 ccggcaggcg gatggcccgc atggtgggat caagcgtggc gaagaggaga tcctgcgcca
     7921 tcacttcagc gccggtcaac cgattgaaca gcgtggattt tccggcattg gtatagccca
     7981 ccagcgccag caccggccag ggcgccttgc cgcgccgctc gcggtgcagg ccgcgggtgc
     8041 ggcgcacctg ctccagctcg cggcggatgc gggccatgcg gtcgcggatc atccggcggt
     8101 ccgcctcgat ctgcgtttcg cccgggccgc ccaggaagcc gaagccgcca cgctggcgtt
     8161 ccaggtgggt ccagctgcgc acaaggcggc cggcctggta atccagatgc gccagttcca
     8221 cctgcaggcg gccttcggcg gtggcggcgc gctcgccgaa gatctcgagg atcaacccgg
     8281 tgcggtcgat caccttgcgc ttcagctttt cttccagatt gcgctgctgc acggccgaca
     8341 gcgcaccatc gacgatgatg agatccgcct cgtgcagatt gcactggatg ccgatattct
     8401 cgatctggcc ctcgccgaac agcgtgcccg gccgcgcctc gcggatcggc atggcaatgg
     8461 catcggcaat gacaaggccg atggccagcg ccaaccccct cgcctcttcc agacgcgcgg
     8521 cgagatctgt ggcggcaggc cgcctgtgga aatcggggca gatgatgagg gcgcgcgcac
     8581 cgcgcgccac ctcaccctgc tcttcgtcga aaaaggtcag gcggaagcgt cctcttcgcc
     8641 gtcccagtca ccggtcaggt ccaccggggt cacgggctgg atggtggaga cggcatgctt
     8701 gtaggccagc tgcacatagc cttcgcgttc cagtagcatg cagaacagat cataggcggc
     8761 cacgcgcccc tgcagcatca cgccgttcac caggaacatg gtgacctgca cggcggcggc
     8821 gcggatgctg gaaaggaaca catcctgcag caggcgggcc ttcttggcgc cttccgcgcc
     8881 attgccgaac agacgcgtgt cgatcggcag gctgggcatg atggtggaga cagcatgctt
     8941 gtaaaccagc tgcgactggc catcgcggcg cagcaggatc gagaaattgt cgaaccaggt
     9001 gacgatgccc tgcagcttca cgcccttcac caggaacatg gtcaccggcg tcttgttctt
     9061 gcgcaggtgg ttcaggaact ggtcctgcag gttctggctg cccttggcgg cactggccgg
     9121 ggcggcgatg gtgtctgcgg gcacggcatc ggccttggtg ctgctgcgcg gcgcgcgggc
     9181 gcgcggggcc ggcgcgggct cggcgtcagc agcaagggtt tcggtggctt cggtggcggc
     9241 ctcggcctct tcggccaagg gctctgccgc cggcttcagc ttgggccgtg cggaaagggt
     9301 acgtccggtc attgactgct cctgttgttg gcggccgcat ttacggtccg cgatctgggt
     9361 ggcgattcac cacccgaacc gatgttcccc atttagccat tcgcaatcgc agcgtaaatg
     9421 gtgaaatcga taacaatccc ctttcggggc aattcagccc tcctcgcctt cctcgtcctc
     9481 gcgccggtcc accatgccca aaagcttcag cttgcggtgc agggcggatc gctccatgcc
     9541 gataaaactg gctgttttcg agatgttgcc ggaaaagcgg cggatctgca cacgcagata
     9601 ttcgcgctcg aaattctccc gcgcctcgcg cagcggcacc cccatcagcg ccgacatgcc
     9661 gctatcgccc ggcagccggc cgtggacgat ctcgccgggc agcatgtcgg gttcgatgcg
     9721 gcccagcctt tcgcgcgggg ccaaaatcac cgtgcgctcc accacattgc gcagctggcg
     9781 cacattgccg ggccattcat aggcctgcag cgcggccatc gcctccatgc cgatttccgg
     9841 cggcggcagg ccctggtcat tggcatagcg ggcgaagaaa tgggccacca gcgcggggat
     9901 atcgtcgcgc cgctcggcca gcgggggcac gctgacgggc accacattca gccggtagaa
     9961 caggtcctcg cggaagcgcc gctcggcaat ttccttctcg aggttgcggc tggtggagga
    10021 gacgacgcgc acatccacgc ggatctgccg gtgcccgccc acgcgcacga agctctgctc
    10081 ggtcagcacg cgcaggatgc gggcctgggt ggagagcggc atatcgccca cctcgtcgag
    10141 ataaagcgtg ccgccatcgg ccatttccag aaggccggca cggatcagct ggccatcctg
    10201 ctcctcgcca aacagctcct cctcgaagcg ttccggcgtg atgcgggcgg aattgacgct
    10261 gacaaaggcg ttgccggccc gggggctcca gctgtgcagc agccgcgcgg ccacttcctt
    10321 gcccacgccc gatgggcccg aaatcaagag ccggctgccg gtgttggcca cccgcttcag
    10381 cgtggcgcgc acatggttga tggcgctgga ggagccggtg aattcctggc tggaaatagt
    10441 gtcgtgccgc aggcgggcgt tctcgcggcg caggcgctcg gtctcggtgg cgcggccgac
    10501 gaggtggagc agccgctcgg tctcgaaggg cttctcgatg aaatccaccg cgccccggcc
    10561 gattgccgcc accgccgtat cgatattgcc atggccggaa aagatgatca ccggcaggtc
    10621 cggctcgcgc tgcttgatct ggtcgagcac ttccagcccg tccatcgggc tgccatgcag
    10681 ccacacatcc agcagcacca ggctgggccg gcgctggtcg atcgcttcca gcgcggcggt
    10741 gctgtcgcca gcggtgcgcg tgtcatagcc ttcgtcgctc aacaccccgg ccaccagctc
    10801 gcggatgtcg cgttcgtcat caacaatgag gatatcgaga gccatcgaac cagcctactc
    10861 catgtctcca gcggccagtg gcctattgcg cggcttgcgc atcgttgagg gcatctgccg
    10921 cgccaggcgc gggcttggcc aacggatcgc gggcaaagcg cagggaaacc tgcgtgccgc
    10981 catcgggagc ggaggcgaag atcatctcgc cgccatgctc ctcgacgatt ttcttgacga
    11041 tggcgaggcc aaggccggtg cccttctcgc gcgtcgtcac ataaggctcg acgatccgct
    11101 cgcggtcctg cggcaggccg atgccattgt cgatcaccat caccagcacg ctgtcggccg
    11161 tgccggaaag ctccagccgg atgcggccgc gataatcggc atcggccgat ttggcccgcg
    11221 cttccaccgc ttccgtggcg tttttcagca cgttggtcat cgcctggcca aactggtggc
    11281 gatcgcaggc gatcagcccg gcctcgccca gcgcggcaaa gctgaagctg atgtcgggcc
    11341 gcgccacttc ctgcaggaac agcgcctggc gagccagatc cgccggatcc tcgccgcgga
    11401 acaccggctt tggcaggcgc gcgaaggagg agaattcatc caccatcttg cgcaggtcgc
    11461 ccacctgccg gatgatcgtc gcggtcagct cctcgaacag ttccggatcg agcaggatct
    11521 gcttgcgata gcggcgcgaa agccgctcgg tcgccagctg gatgggggtc agcgggttct
    11581 tgatctcatg ggcgatgcgg cgggccacat cgctccacgc cgcctgccgc tggtcgagca
    11641 gctggcgggt gatatcctcg aaggtgatca catggccgcc gcgattgtcg gtcaccttca
    11701 ccgccagcgt cagcagttcg ccgcccttgc catgctgcac gatcgcctcg gaaacatgcg
    11761 ccctgccctg cccgccgctg ccctcatggc cggcaagcgc ggccagctgc ggtgccagtt
    11821 caaccagcgg gtggccgatg ggcggcgcca gctgcgcctc ctgcggatcg agcagcagct
    11881 tttgcgccga ggaattcatc agccggatgg ccccttcctc gtcgagcgag atgatccccg
    11941 ccgtcaccga ttcgagcacc gcctcgatga aggcgcggcg ctcatccagc tgctggttgg
    12001 cgcgcagcac ggccgccgtc tgccgctcga tctggctggc catgcggttg aaggcgcgcg
    12061 tcaccaggcc aatctcgtcg aagcccacgc gcccgcccac ccgcaaggtg taattgccgc
    12121 tgcccacctg cctggccgcg atcgccagat catagagcgg ctgcacctgc ttgtcggcaa
    12181 agcgcaggcc aaaccacacc gccagcccca ccagcgccag cgaggcaatg aacagcgcca
    12241 cgttgaagcg cagttgcagc agccgcgcgc gctgggtcag cacctgatag gcgcgcacga
    12301 tattgtcggc ccgcttgccc tggctgatgg ccagaagatc tgatttgcgg gcggcgtaga
    12361 gataaatacc ggcgcgccgg tcgatcggcg tcatcgcctc gatgcgatcg gccgtggcgt
    12421 tgacgatgat cggctcgccg gcatcgatcc ggcgcagctc ggcctcggtc acccaatcgc
    12481 gggggcggcc cgagggatcg acataggcgg cggtgttgag ctggccattg ggccccttct
    12541 gcaggatcgc cacgtcgtcc agcttgcgct gcactacctg atagaagaag ccctcgcgaa
    12601 attccgggct caccaggctg acccggctca gcgcatcgcg cacgtcatag gccatcgcca
    12661 ccgtgttgtc cgacacgtcg cgctgggtct gctcgtaata gccgcgggcc agcttgttgg
    12721 cgttttccag cagcccgcgc gaattgtcgg aaaaccagaa ttccacgccc gactggaaca
    12781 gccaggaagc gaaaatcgcc accagcagcg tcggcaaagc ggcgatcacc gagaacagga
    12841 acaccagctg cacatgcagc cgccccttgc cgccaaaacg cgcccgcgcc cggttcagcg
    12901 ccatgtgccg gccgatcagc atcaggatcg ccatgcccgg caccagcgtg cccaccagca
    12961 gcgtggccgt gaggccagag ggcagcagct cgccgcgggc atcctgatcg gccagcgccc
    13021 gccacgacat cagcgtggcg gccaggaagg ccaccaccga gatcacttcc agccagcgga
    13081 agatgccatc gcgccgcagc atcagctgca gccggcgcca ccagcggggt ggccggcgcg
    13141 gaacaagggg cggcgcggtg ttcatggttg cctgattaca acgctagtgt tgcgtttttg
    13201 caagagcgaa tttgtccccc ctgccctttg cgtggccagc tatccgtcgc gccgcaggaa
    13261 actgtcgggg tcgatcgcca gatcgttcag ccgcttgcgc aaggtgttgc ggttgatgcc
    13321 cagcacctgc gcggcgcgca gctggttgcc gccggtctgc cgcaggatgc cggtgaacag
    13381 cggccgctcg aaagcggcca gcgccgtgtc atagaccagg ccctggggcg ggcgggtctc
    13441 gtccagccag tgcgccaccg cttcctccag ccccacaggg ccgggcgcgg gcgcttctgc
    13501 gcgggcgttc tgctccagca tcggcagcag gctggcctca tccaccagat cctcacgcgc
    13561 cagcagcgct acgcgataga tgaaattgcg caattcgcgc acattgccgt gccagggctg
    13621 acgctgcagc agccgcgccg catcggccga aagatgccgg cgcggcaggc cttcctgcgc
    13681 cgccagggcc aggaaatgcc gggccagtgc ctcgatatcg tcggcccggg cgcgcaaggg
    13741 cggcaggttg atgggcacca cattgatgcg gtagtaaaga tcctcgcgga actggccggc
    13801 ggcgatcatc ggcaccagat ccttgttggt ggcagcgata atgcgggcat cgatgcgcac
    13861 ttcctcgcgc cctcccaccc ggcggatgct gcccgattgc agcgcgcgca gcaggcgggt
    13921 ctgggcctgc atcggcatgt cgccgatttc gtcgaggaag agcgtgccgc cggcggcctg
    13981 ctcgaacttg ccgatatggc gggcgacggc gccggtgaag gcgcccttct catggccaaa
    14041 cagctcgctc tcgatcagct cggccgggat ggcggcggtg ttcaccgcca cgaagggccc
    14101 ctcgcgccgg tggcccagct tgtggatggc ctcggccacc agttccttgc cggtgcccga
    14161 ttcgcccagg atcagcacgg tgaggtcgtt cttcagcacc cgcgtgatca tgcggtaaac
    14221 cgcctgcatc gaggcgctgc gccccaccag cggcagccct tgcgcctcgg tgtcggcctc
    14281 cacctcgccg ggggcccggc cggcagagcc ggtggcctgg cgcacggtgc gcaccagctc
    14341 gtcgatgtcg aacggcttgg ggaaatattc gaaggcgccg ccatcggtgg cgcgcaccgc
    14401 cgtatccagc gtgttctgcg ccgacagcac gatcaccggc atatgcgggc gcaggctgcg
    14461 cgccagcgcc agcgtttcca gcccgtcgcc atcggtcagc atcacatctg tcaccagcgc
    14521 ggcataggcg tgcaggctca gcagccggtc gcgctcggcc acgctgtcgc agcgggccac
    14581 ttcatagcct tccgcctcca gcgcggcggt gatcacgatg gcgatggatt catcatcctc
    14641 caccagaagc acggtcatca tctgtccttt gttctcagcc cgccaactgg gcctgtttgc
    14701 gcgaccgccc cggggccacc ggcaggtgca ggcggaaatg ggtccagccg ccgcgctcgt
    14761 cgcggtcgtg ggtgatgcgc ccgttcatgt cgcgcaccag cttgcgcacc agcgccagcc
    14821 ccagcccctg cccggatttc ttggtggaga cgaaaggctc gaagatatgg tcgcgcatgt
    14881 ggggggcaat gccggggcca ttatcgctga tgcgcacctc gatcggcagg cgcacggcct
    14941 ggcgcccgcc ggtggccagc aactgcaggc ccgagacaaa gcgcgtgcgc accaccagcc
    15001 ggggatcggg ctgatcggcg caggcatcgc ggccattggc cagcaggttg atcagcacct
    15061 gggtcaacgc atgggcattg cccagcaccg gcggcagcga ggggtcgaac tcttcctcga
    15121 tgcgcggcgc gcggccgctg gcggtgagga tggcgctggc ccggcggatc acctcatgca
    15181 ggttgcacgg ctccaccggc tccaccgtgc ggcgcgagag cgtctgcatc tggtcgatca
    15241 gcttggcgat gcggtccact tcctcggcga tcagcgtggt cagcgcgcgg tcgtggccat
    15301 ccaccttgcg ggccagcaat tgcgccgcgc ccttgatgcc ggccagcggg ttcttgatct
    15361 catgcgccag gatctcgggc gcgcgcacca tgccttcctc gctgcccacg gtgttctcgc
    15421 ccaatgcctc ggtcgcggcg ctgtcgtgca gggtcagcac ctgccagccc tcggcatcgg
    15481 cgatgggggc cacggtcaca tccagccggc ggctgtcctt gcccttcacc accacatcga
    15541 tctggcgggc cgagagcggc gcatcggcgc ggtgcaggcg ctcggtcagc aggggctcgc
    15601 cgaaagagag cacatcgccc agcgccctgc cctgcagccg gcggaagctc tggctgagga
    15661 actgttcggc cgccggattg gccgcggcaa tgcgcagatc cggccccagg gtgatgaccg
    15721 ccagcggcag gcaggccagc tggcggcggg catcgggcag ctctgcgcgg gcgcgggccg
    15781 gcggggctgc cgcctcgctc acgccgcgcg ggctttcagg aaggggcggt agaactcggc
    15841 aagcgtggcc ttcacctcgg ccggatcgtc catgaagttc accttgttgc gcaggtctgc
    15901 cgcgccgggg atgcccttga tgtaccagcc cagatgcttg cgcgagaggt tcactccggc
    15961 gcggatgtcg taaagcgcga ggatatcctc gtaatgctcc atcatcgtgg cgaattgttc
    16021 gtcgagcgtg ggatcatcga gcagcacgcc ggtcttcagc cagtgcatca cctggcccag
    16081 cagccacggc cggccatagg ccccgcgccc gatcatcacc ccgtcggcgc ccgattgcgc
    16141 catggcctgg gccacatcgg ccacattgca gatatcgccg ttgacgatga ccggcacctg
    16201 caccgcttcc ttcacccggc gcacaaacgc ccagtcggcc gtgcccttgt acatctggtt
    16261 gcgggtgcgg ccgtgcacgg tgatcatccg cgcgcccaga tcctcggcaa tgcgggccag
    16321 ttccggggcg ttcaggctgt cgtggcacca gcccatgcgc atcttcacgg tcaccggcac
    16381 cttcaccgcc ttcaccgtcg ccgcgatcag ccgggcggcc agcggcaggt cgcgcatcag
    16441 cgcagagcca gcctcgccgt tcaccacctt gcgcaccggg cagcccatgt tgatgtcgat
    16501 gatcgccgcg cccttgtctt cagacagctt ggccgcttcc gccatctgtt ccggctcgca
    16561 gcccaccagc tgcatcgaca ccggctcctc ggccggatcc cacgccgcct tctggatcga
    16621 ctggcgcgtc tcgcggatgg cggccgggct ggcgatcatc tc
//
LOCUS       DASRYE010000302         6568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_169647,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000302 DASRYE010000000
VERSION     DASRYE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169647"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1278)
                     /locus_tag="VFF98_15565"
     CDS             complement(70..1278)
                     /locus_tag="VFF98_15565"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HZU65104.1"
                     /translation="MEDEFYRIKRLPPYVIAEVNGMRAAARAAGKDIIDLGMGNPDLP
                     PPQHVIDKLCEVAQKPSAHGYSASKGIPGVRKAQANYYGRRFGVELDPDTEVVMTMGS
                     KEGLASMATAMTAPGDVVLAPNPSYPIHTFGFIIAGATIRSVPTTPDEEYWRSLDRAM
                     AFTVPRPSILIVNYPSNPTAEVVDLAFYERLVAWAKENKVWILSDLAYSELYYDGNPT
                     VSILQVPGAKDVAVEFTSMSKTFSMAGWRVGFAVGNKRLIAALTRVKSYLDYGAFTPI
                     QAAACAALNGPQDIVEANRQLYQKRRDVMVEAFGRAGWDIPSPKASMFAWAPLPPALA
                     SMGSLEFSKRLLTEAEVAVAPGVGYGEDGEGFVRIAMVENEQRLRQAARNIRRFLMNH
                     GVNVPAAKAG"
     gene            1462..3477
                     /locus_tag="VFF98_15570"
     CDS             1462..3477
                     /locus_tag="VFF98_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU65105.1"
                     /translation="MAEDTTAPTTDQPANPFAALQEMWASLLPAEAEVAQEAIGQMVA
                     PLMSAWGSSAEALAKMAGPALPHLAAYLPKPDADPAEVAGDVAGEAAHWADVSTRLQA
                     MWLEFQNEQALKAAGKPPHFLDPAQWLPMAQKWYQQVPLAHPEVQRKIWEDAQGLWEG
                     VLGSYGIGPRAGEEAHATALPRHDKRFADARWRDNPFFALVHQAYLMVAEQMVALADS
                     VEGLEPEKKEQLRFATRVAVDALSPDHFPLTNPLVLEKALETRGESLVKGLSHLLDDL
                     RRGQLTHTDPNAFRLGENIAATPGKVVHESRLYQLIQYTPTTDKVMTTPLVIFPPWIN
                     RFYILDLNPRKSFVKWALDQGLSVFMVSWKSADASIADVTWDDYIAAEIEAIDVACQR
                     LKVPSAHTIGYCVAGTTLAATLAVLARKGEQGRVASATFFTAQVDFEKAGDLKNFIDD
                     QQIAALQALATDGFIDGRYLAATFNLLRGNDLIWNYVVQNYLLGEDYPAFDLLYWNGD
                     STNLPAGWHSAYLKDLYRDNRLVVPDSLSALGVPIDLTRITTPCYIQAGREDHIAPAE
                     SVWRFLHHLPHAPHTFLLAGSGHIAGVVNPPAAHKYQYWTNGAEVDSLAAFMAGASEH
                     PGSWWPHWLEWLRGLDGTSVSAKGKRVPGGRGDKAIEEAPGSFVRQR"
     gene            3886..4641
                     /locus_tag="VFF98_15575"
     CDS             3886..4641
                     /locus_tag="VFF98_15575"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZU65106.1"
                     /translation="MAAKPVKAAPVAEPTAPVPAKPPVLAEPTPPAPEPAPPVAEPPV
                     AEPVAAAPVAPEPAPVAAPEPAPPVAQPAAASVPEPVGAAEPAEPATSAVPPATTAKP
                     KENPMSTFSFDFSEVFKSAFTDFQEKAKAAYEKSTSALGDYNDFAKGNVDALVESSKI
                     LAAGLQELGSTLVADSRSNFESLTAEAKELAASKTPTDFFKLQSELLKKHFDSAVAAS
                     SKQSEALLKLASEVSAPLSNRVSIAVETIKKAA"
     gene            4884..5264
                     /gene="clpS"
                     /locus_tag="VFF98_15580"
     CDS             4884..5264
                     /gene="clpS"
                     /locus_tag="VFF98_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZU65107.1"
                     /translation="MTAQACVEGARVGAAFVAGPDREGSGDGANQIGLATKARAKPKK
                     PSQFKVLMLNDDYTPMEFVVMVLKRFFHMDLEQATRVMLHVHQKGVGVCGIFPYEIAE
                     TKVNQVMDYARANQHPLQCTLEKA"
     gene            5474..>6568
                     /gene="lptF"
                     /locus_tag="VFF98_15585"
     CDS             5474..>6568
                     /gene="lptF"
                     /locus_tag="VFF98_15585"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZU65108.1"
                     /translation="MKQFTALDRYIFRLVLMPMLAVFCIAASLLLLDKMLRLFDFVAT
                     EGGPVSVVFQMLASMMPEYASLAIPLGLMLGVLMAFRKLAVSSELDVMRAVGLSYTRL
                     LKVPYMLAIVLAGVNLMIVGYVQPWSRYYYERLTFELRSGALGASIKVGEFNTLKDRM
                     SLRIEQSRDDGRELIGIFARVMEQNGEVLSISARSGRFLALRENPDTVILRLNDGQIV
                     QDPADQAPRVLSFTTHDLPIDLPALEKFRQRGGAEREYVLPELLQIGWVKNTTDQERA
                     MAKASLNYRLAEVVMVLMLPLLGVALAVPPKRSTSSLGVFLSIVMVVAYHKVNEYGQS
                     VASLGRIDPALALWGPFAVFAVIIGWMYWRI"
BASE COUNT         1107 a         2083 c         2231 g         1147 t
ORIGIN      
        1 cggcgcaggt tagcggataa ggcccaaggg gcggggcacc agcgcggcgc cccgccccct
       61 gttcagggtt cagccggcct tggcggcggg cacgttcacg ccgtggttca tcaggaagcg
      121 gcggatgttg cgggcggcct ggcgcaggcg ctgctcgttt tccaccatgg cgatgcgcac
      181 gaagccctcg ccatcctcgc catagccaac gcccggcgcc acggccactt cggcctcggt
      241 cagcagccgc ttggaaaatt ccaggctgcc catcgaggcc agcgccggcg gcagcggcgc
      301 ccaggcaaac atgctggcct tggggctggg gatatcccag ccggcgcggc cgaaggcttc
      361 caccatcacg tcgcggcgct tctggtagag ctggcggttg gcctcgacga tatcctgcgg
      421 gccgttgagc gcggcgcaag ccgccgcctg gatgggcgtg aaggcgccat aatcgagata
      481 ggacttcacc cgggtgagcg cggcgatcag ccgcttgttg cccaccgcaa agcccacgcg
      541 ccagccggcc atcgagaagg tcttcgacat cgaggtgaat tccaccgcca catccttggc
      601 gccgggcacc tgcagaatgc tgaccgtggg gttaccgtcg tagtacagct cggaataggc
      661 aaggtcggac aggatccaca ccttgttttc cttcgcccag gccaccagcc gctcgtagaa
      721 ggccaggtcc accacctcgg cggtggggtt cgagggatag ttgacgatga ggatcgaggg
      781 gcgcggcacg gtgaaggcca tggcgcgatc aaggctgcgc caatattcct cgtcgggcgt
      841 ggtgggcacc gagcgaatcg tggcgccggc aatgatgaag ccgaaggtgt ggatgggata
      901 ggacgggttg ggcgccagca ccacatcgcc gggcgccgtc atggccgttg ccatcgaggc
      961 caggccttcc ttgctgccca tcgtcatcac cacttcggta tcgggatcaa gctccacacc
     1021 gaagcggcgg ccgtaatagt tggcctgcgc cttgcgcacg ccgggaatgc ccttggaggc
     1081 cgaatagccg tgcgcgctgg gcttctgggc cacttcgcac agcttgtcga tcacatgctg
     1141 cggggggggc agatcggggt tgcccatgcc caggtcgatg atgtccttgc ccgcggcgcg
     1201 tgctgcggcc cgcatgccgt tgacttcggc gatgacatag ggcggcaggc gcttgatgcg
     1261 gtagaactcg tcttccatga ctcttttcgc aattccatga gatcggcggc ggaatgtgcc
     1321 gcccaccacc ccctacgcct gtcgggcggc tctggcaaat ggcgcgtcga tcgcccataa
     1381 gcgggggtgc aaagggaaag tgggtgttcg cccggccggg gcggatgccg ccctgtctgc
     1441 gcgagaagga gcctgcgcct gatggccgag gatacgaccg cccctaccac cgaccagcct
     1501 gccaacccgt ttgccgccct gcaggagatg tgggccagcc tgcttccggc cgaggccgaa
     1561 gtggcccagg aagcgattgg ccagatggtg gccccgctga tgagcgcctg gggctcttcg
     1621 gccgaggcgc tggcgaaaat ggccggccct gcgctgccgc atctggccgc ctatctgccc
     1681 aagcccgatg ccgatccggc cgaagtggct ggcgatgtgg ctggcgaggc cgcgcattgg
     1741 gccgatgtgt cgacccggct gcaggcgatg tggctggaat tccagaacga gcaggcgctg
     1801 aaggcggccg gcaagccccc ccatttcctc gatccggcgc agtggctgcc gatggcgcag
     1861 aaatggtatc agcaggtgcc gctggcccac cccgaggtgc agcgcaagat ctgggaagat
     1921 gcgcagggcc tgtgggaagg ggtgctgggc agctatggca ttggcccgcg tgccggcgag
     1981 gaagcgcatg ccacggccct gccgcgccac gacaagcgct ttgccgatgc ccgctggcgc
     2041 gacaacccct tcttcgcgct tgtgcatcag gcctatctga tggtggccga gcagatggtg
     2101 gcgctggctg attcggtgga ggggctggag ccggagaaga aggagcagct gcgctttgcc
     2161 acccgcgtgg cggtggatgc gctgagcccc gaccatttcc cgctgaccaa tccgctggtg
     2221 ctggaaaagg cgctggaaac gcgcggcgaa agcctggtga aggggctttc gcacctgctg
     2281 gacgacctgc gccgcggcca gttgacccac accgacccca acgccttccg cctgggcgag
     2341 aatattgccg caacgccggg caaggtggtg cacgaatcgc gcctgtatca gctgatccag
     2401 tatacgccga ccaccgacaa ggtgatgacg acgccgctgg tgatcttccc gccgtggatc
     2461 aaccgcttct acatcctcga tctgaacccc aggaaaagct tcgtgaaatg ggcgctggac
     2521 cagggcctca gcgtattcat ggtgagctgg aaatcggccg atgccagcat cgccgatgtg
     2581 acctgggatg attacatcgc cgccgagatc gaggcgatcg acgtggcctg ccagcggctg
     2641 aaggtgcctt ctgcccacac catcggctat tgcgtggcgg gcaccacgct ggccgccacg
     2701 ctggccgtgc tggcgcgcaa gggcgaacag ggcagggtgg ccagcgccac tttcttcaca
     2761 gcgcaggtgg atttcgagaa ggcgggcgac ctcaagaact tcatcgacga ccagcagatc
     2821 gccgcgctgc aggccctggc taccgacggc tttatcgacg ggcgctatct ggcggccacc
     2881 ttcaacctgt tgcggggcaa tgacctgatc tggaactatg tcgtccagaa ctatctgctg
     2941 ggcgaggatt atcccgcctt cgacctgctc tactggaatg gcgacagcac caacctgccg
     3001 gccggctggc acagtgccta tctgaaagac ctgtatcgcg acaaccggct ggtggtgccc
     3061 gattcgctgt cggcgctggg ggtgccgatc gatctcacgc ggatcaccac gccctgctat
     3121 atccaggccg gccgcgagga ccatatcgcg ccggccgaaa gcgtgtggcg cttcctgcac
     3181 cacctgccgc atgcgccgca caccttcctg ctggccggat cgggccatat cgccggcgtg
     3241 gtgaacccgc cggcggcgca caaataccag tattggacga atggcgccga ggtcgattcg
     3301 ctggcggcgt tcatggccgg ggccagcgag caccccggca gctggtggcc gcactggctg
     3361 gaatggctgc gcgggctgga tggcaccagc gtttcggcga agggcaagcg ggtgcccggc
     3421 gggcgcggcg acaaggcgat agaggaggcg ccgggcagct tcgtcagaca gcgctaaagc
     3481 ttcttcgcac ttgcacaata gcctggcggg gaacgcttct ccgccgggct ggggcgcttg
     3541 tgggaaaggc ccatttttgc gccagatcat tgcgttttta tggcgaaccg cgaggcccgt
     3601 actttgcgct aggggatttt gctgcggcgc acaattgcgc ttgactccgg cttcggaaac
     3661 atggcatttg tgcactgcat caaaaaggcg gggcggaatt gattcgcacc tgcccgggca
     3721 ggggttcgaa ccgcaatggc taatgaaccg gaagacaagc ttggcaggaa aacgaagccc
     3781 cgcgtgggca agcctgcctc cagtgtgaaa gcgccggcca ggccggttgt atccaaggca
     3841 gctgttgagc cgcccgtggc ggaaagcgtg gccaaacccg cgcccgtggc ggccaagcct
     3901 gtgaaggcag cgccggtggc agagcccacc gcgcctgtgc cggccaagcc gccggtgctg
     3961 gcagagccca cgccgcccgc gcccgaaccg gcccctcccg tggcagaacc cccggtggcg
     4021 gaacccgttg ccgccgcgcc tgtcgcgccg gagcctgctc cggtggccgc gcccgagccg
     4081 gcgccgcctg tggcacagcc tgccgctgca tccgttcccg aacctgttgg cgctgctgaa
     4141 ccggcagagc ctgctacgtc tgccgtccct cctgccacca ccgccaagcc caaggaaaat
     4201 cccatgtcga ccttcagctt cgatttctcg gaagtgttca agtctgcctt caccgatttc
     4261 caggaaaagg ccaaggccgc ctacgaaaag tccaccagcg cgctgggtga ctacaatgat
     4321 ttcgccaagg gcaatgtcga tgccctggtc gaatcctcga agatcctcgc tgccggcctt
     4381 caggaactgg gcagcacgct ggtggccgac agccgcagca acttcgagtc gctgaccgcc
     4441 gaagccaagg aactggccgc ttcgaagacc ccgaccgact tcttcaagct gcagagcgaa
     4501 ctgctcaaga agcacttcga cagcgctgtg gccgccagct cgaagcagag cgaagcgctg
     4561 ctcaagctgg ccagcgaagt gagcgcgccg ctgtcgaacc gcgtgagcat cgcggtggaa
     4621 accatcaaga aggccgcctg aggctttccg gcccgggcgc gcggcacagg gccggcgcca
     4681 gggccgcagc agacaggctg gccttacggg gctggccgcg gaagggccgg gcgcgatgcc
     4741 cggccttttt cgcatccccc gcgggggaaa cggggcggaa aatggggcct tgtgccgctt
     4801 tgggccctgc ggcgtgccag cttggccctt gcggcgcctt gttttgcccc cccgctttgc
     4861 ggtattgttc gcggatgcgt gtgatgactg ctcaggcctg tgtcgaaggc gcccgtgtgg
     4921 gcgcggcgtt tgttgccggc cccgatcggg agggcagcgg cgatggtgcc aaccagatcg
     4981 gccttgccac caaggcccgc gccaagccca aaaagccaag ccagttcaaa gtgttgatgc
     5041 tgaacgacga ctacaccccg atggaattcg tggtgatggt gctgaagcgt ttcttccaca
     5101 tggatctgga acaggcgacg cgggtgatgc tgcatgtgca tcagaagggc gtgggagttt
     5161 gcggcatttt tccctacgaa atcgccgaga ccaaggtgaa tcaggtgatg gattatgccc
     5221 gcgccaacca gcaccccctg caatgcacgc tggaaaaggc ctgattcgcg ccgtttttcc
     5281 cgcacatgcc gcccgccttg ggtgagaatc cgcaaggcgg gcgcacgcgg cctcttgcag
     5341 gcgggcgcgt ggctgcttag tgcctcgggc aacacgggcc cgcgtatcgg cggtgctccg
     5401 gccagcgctc cggcgccccg gccgggcgcg gctggcggcc cacctttgat gaaagcgcag
     5461 cgccggttcc cttgtgaaac agttcaccgc tcttgaccgg tatatcttcc gcctggtgct
     5521 gatgccgatg ctggcggtgt tctgcatcgc cgcctcgctg ctgctgctcg acaagatgct
     5581 gcgcctgttc gattttgtgg cgacagaggg cggcccggtc tcggtggtct tccagatgct
     5641 ggccagcatg atgccggaat atgcgagcct tgccatcccg ctggggctga tgctgggggt
     5701 gctgatggcc ttccgcaagc tggccgtttc cagcgagctg gacgtgatgc gcgcggtggg
     5761 cctcagctat acgcggctgc tcaaggtgcc ctatatgctg gcgattgtgc tggcgggcgt
     5821 gaacctgatg atcgtcggct atgtgcagcc ctggtcgcgc tattactacg agcggctgac
     5881 cttcgagctg cgttcgggcg cgctgggcgc ctcgatcaag gtgggcgagt tcaacacgct
     5941 gaaggaccgc atgagcctgc gcatcgagca gagccgcgat gatggccgcg agctgatcgg
     6001 cattttcgcc cgggtgatgg agcagaacgg cgaggtgctg tcgatcagcg cgcgctcggg
     6061 ccgcttcctg gccctgcgcg agaaccccga tacggtgatc ctgcggctga acgacgggca
     6121 gatcgtgcag gatccggccg atcaggcgcc gcgggtgctc tccttcacca cccatgatct
     6181 gccgatcgac ctgccggcgc tggagaaatt ccgccagcgc ggcggggcgg agcgcgaata
     6241 tgtgctgccc gaactgttgc agatcggctg ggtgaagaac accaccgatc aggaacgggc
     6301 gatggccaag gccagcctca actatcgcct ggccgaggtg gtgatggtgc tgatgctgcc
     6361 gttgctgggg gtggcgctgg cggtgccgcc caagcggtcc acgtcctcgc tgggggtgtt
     6421 cctgtcgatc gtgatggtgg tggcctatca caaggtcaat gaatacggcc agtctgtggc
     6481 cagcctcggg cgcattgatc cggcgctggc gctgtggggg ccgtttgcgg tgttcgcggt
     6541 gatcatcggc tggatgtatt ggcgcatt
//
LOCUS       DASRYE010000303         5416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_33374,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000303 DASRYE010000000
VERSION     DASRYE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33374"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..835)
                     /locus_tag="VFF98_15590"
     CDS             complement(167..835)
                     /locus_tag="VFF98_15590"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HZU65109.1"
                     /translation="MATGLALLASPLHAEVTPQAGAGDPHIQQVAYDPQQVVGLHVAL
                     GYAVTVELAPDERVETVTLGDANGWAVQVNHRADHLVVKPQAAAPPTNLTVITDQRTY
                     NFSLYGAGRGEGVQPYLLSFTYPQPAPLAAPAPAVGHYRLQGDRALWPSRLEDDGRFT
                     RLVWPAAVEMPAVYAEGADGRQALVNGVMREGAYVIEGVAPRLLFLRGGHRASALRQA
                     EPAP"
     gene            complement(847..1791)
                     /locus_tag="VFF98_15595"
     CDS             complement(847..1791)
                     /locus_tag="VFF98_15595"
                     /inference="COORDINATES: protein motif:HMM:NF016238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZU65110.1"
                     /translation="MRGHQVKGLGMASEEGDGRARYYARAQGWAQDREGARRRSARAA
                     WIVAGVAATLAGLEALALVALAPLKTVVPYTLLVDRNTGFAQALEGTGLAAIRPDAAL
                     TQALLAQYVIARESFDIATIAEQYRKVALWSAEGARADYLAQMPAANPASPLARLGRG
                     AVLSVRVESVSMIAADTALVRFANDLALPGRPRGATAYWVAVVRYRFAGAPMAVEDRL
                     VNPLGFAVTSYRRDAEAPPVAEPPAPVATATAAVVAAPVPAPAVAAMPVQPPSPAAPA
                     APAAVMPQAGGGLSPGLARRRYPWAGHGAGGRIPEAGQ"
     gene            complement(1788..2960)
                     /locus_tag="VFF98_15600"
     CDS             complement(1788..2960)
                     /locus_tag="VFF98_15600"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZU65111.1"
                     /translation="MMEACAAFDPAAPLAGALTGFVDCQARALGAAGWAALGPGTGWG
                     AALSGLMALAVAGLGYRLLLGAGGGLRAALGLALRLGLVLALAGQWPAWQALAYDLAV
                     DGPGQLSGWLPGAQGPADLARRVDGVAQGVEALMQPAPAAPGAGGPAEAPWMRSARRA
                     EGVFMISTLAALVAPRGVIGLLLALGPVFTGFALFGATQGLFAGWARVLLGAALAQVA
                     LGLVVQAELAVVEPELQALAAIVVAGGAAQGAVQQMGEVAWVFAGLALAVVLALAGAT
                     AALRWPHGLWRPAGALPQARAEPAVAVPQETPVPALLAAPAARARRTADAVLALERRE
                     AALALPMAQGAGPVPAVQQGRGGEGAAGPVTMGLGQAGRRGGQRVSAAARARDGIR"
     gene            complement(2957..5368)
                     /locus_tag="VFF98_15605"
     CDS             complement(2957..5368)
                     /locus_tag="VFF98_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HZU65112.1"
                     /translation="MMAQARLMPDFAREAPAGDRLPYAAQIDDHTILLRDGRLMQVIA
                     LEGLMAETADTAEINYRKDLRAAMLRAVGSPRLALYHHILRRRVRPDLGGDFADAFSR
                     GLDARWQARLAARQLYANAQFLTLVRRAPGRQAAGLAGRLAGGWRRRAGRRAAPALLA
                     AECRALDAAAEQMLAALGHYGARRLAVYDTPHGPCGEALEFLAALYNAELRPVRLPEG
                     DVGDYLPWRRVSFGADTLELGPAGGQGQVSLGAIVSIKDYPGETMPGMLDDLLRLPCE
                     MVVSQSFAFVERGESLARMNLALRRLRAADDEALSLRAELAQAKDDVAAGRALYGEHH
                     MSVLVRGVDQAAVDGAVAEVQGALADLGIVSVREDAGLELAFWAQFPGNFRFIARRAL
                     VGTANFAAFASGHDFPQGQARGNHWGDAVTLFETTAAGPYHFNFHRGDLGNFTVIGPS
                     GSGKTVIVNFLLAQARRFDPRIVFFDKDRGAELFIRAIGGRYEVLRPGHPSGLNPLLL
                     PDNAANRRFLADWLACLAGAAQQPLSPAEARAIEEAVAANAAAPVNMRRLSCLVALLR
                     GGQRPEEGDLYTRLRPWWGDGVHGWLFDHPPGPDGADGLDLACPTLGVDMTRLLDDPV
                     LRTPAMMYLFHRVDERLDGRPAIIVVDEGWKALDDPVFAHHIRDWEKTIRKRGGILGF
                     VTQNAGDALASRIAGAIVEQTATQIFTANPRARAEDYGDGFGLTAHECALVRSLPDNG
                     HCFLVKQGGQSVVVRLNLAGEADLLAVLSGREASLRRFEAICGEVGPDPADWLPVLAG
                     AGGAG"
BASE COUNT          867 a         2279 c         1653 g          617 t
ORIGIN      
        1 cccgcagcgg ccacgccgcc ctgccgcgcc tgccgccgcc cctccagcca gaggaacagc
       61 cccagcgcca gcgccgccgc cagcaccgcc accagccaca gcggcagccc ttctggcgca
      121 gcggccacct gcggccggcc atcctgcctg cgcccatcgc gcccgctcat ggcgccggct
      181 ccgcctggcg cagggcgctg gcccggtgcc cgccgcgcag gaacagcagc cgtggcgcca
      241 ccccctcgat cacataggcc ccctcgcgca tcaccccgtt gaccagcgcc tgccggccat
      301 cggcgccctc ggcataaacc gccggcatct ccaccgccgc cggccagacc agccgggtga
      361 agcggccatc atcctccagc cggctgggcc acagcgccct gtcgccctgc aagcgataat
      421 ggcccaccgc cggcgcgggc gcggccagcg gcgccggctg cggataggtg aagctgagga
      481 gatagggctg caccccttcc ccccgcccgg caccatagag cgagaaatta tacgtgcgct
      541 gatcggtgat cacggtgagg ttggtgggcg gggcggcggc ctgcggcttc accaccagat
      601 gatcggccct gtggttcacc tgcaccgccc agccattggc atcgcccagc gtcaccgtct
      661 ccacccgctc gtccggcgcc agttccaccg tcacggcata gcccagcgcc acatgcagcc
      721 ccaccacctg ctgcgggtca taagccacct gctggatatg cggatcgcca gcgccggcct
      781 gcggcgtcac ctcggcatgg agggggctgg ccagcagcgc caggcctgtg gccaccagcc
      841 accgcctcac tgccccgcct ccggaatgcg gccgccagcg ccatggcctg cccagggata
      901 gcggcggcgc gccagccccg gtgacaaacc gccgcctgcc tgtggcataa ccgccgccgg
      961 ggctgctggc gccgccggcg atggtggctg cactggcatc gcggccaccg cgggcgccgg
     1021 aaccggggcc gccaccaccg ctgccgtagc cgttgccaca ggcgcaggcg gctcggccac
     1081 cggcggcgcc tcggcatcgc ggcgatagct tgtcaccgca aagcccaacg ggttcaccag
     1141 ccggtcctcc accgccatcg gcgccccggc aaagcgatag cgcaccaccg ccacccaata
     1201 ggccgtggcc ccacgcggcc ggcccggcag cgccagatcg ttggcaaagc gcaccagcgc
     1261 cgtatccgca gcgatcatcg acacgctttc cacccgcacc gacagcaccg ccccgcgccc
     1321 cagccgcgcc agcgggctgg ccggattggc cgccggcatc tgcgccagat agtccgcccg
     1381 cgccccctcg gccgaccaca gcgccacctt gcgatattgc tcggcaatgg tggcgatatc
     1441 gaagctttcc cgcgcgatca catattgcgc cagcagcgcc tgggtcagcg ccgcatcggg
     1501 cctgatggcg gcaaggccgg tgccttccag cgcctgggca aagccggtgt tgcgatcaac
     1561 cagcagcgtg tagggcacca ccgtcttcag cggggccagc gccaccagcg ccaaagcctc
     1621 cagcccggcc agcgtggccg ccacgcccgc cacgatccac gccgcccggg ccgagcggcg
     1681 gcgcgcgccc tcccggtcct gcgcccagcc ttgcgcccgg gcgtaatagc gggcgcgccc
     1741 atcgccctcc tcgctcgcca tgcccagccc tttcacctga tgccccctca tctgatgcca
     1801 tcccgcgcgc gggccgctgc cgaaacgcgc tggccgcccc gccgccccgc ctgcccaagg
     1861 cccatggtca cagggccagc cgccccctcg ccgccgcgcc cctgctgcac cgccggcacc
     1921 gggcccgccc cttgcgccat gggcagggcc aaagccgcct cccgcctctc cagcgccagc
     1981 acggcatcgg cagtgcgccg cgcgcgcgcc gcaggcgctg ccagcagcgc cggcacgggc
     2041 gtttcctgag gcacggccac cgccggctct gcccgcgcct gtggcaaagc ccccgccggc
     2101 cgccacaagc catgcggcca gcgcaaggcc gccgttgccc cggccagcgc cagcaccacc
     2161 gccagcgcca gcccggcaaa cacccaggcc acctcgccca tctgctgcac agcgccctgc
     2221 gccgcgccac cggccaccac aatggccgcc agcgcctgca gctcaggctc caccaccgcc
     2281 agttcggcct gcaccaccag ccccagcgcc acctgcgcca gcgccgcccc cagcaacacc
     2341 cgcgcccagc cggcaaacag cccctgcgtg gcgccaaaca gcgcaaagcc ggtaaacacc
     2401 ggccccagcg ccagcagcag gccgatcacc ccgcgcggcg ccaccagcgc ggccagcgtg
     2461 ctgatcatga acaccccttc ggcccggcga gcgctgcgca tccacggcgc ctcggccggg
     2521 ccgccggcac cgggcgctgc gggcgccggc tgcatcagcg cctccacccc ctgcgccacg
     2581 ccatccaccc tccgcgccag atccgccggc ccttgcgccc ccggcagcca gccggaaagc
     2641 tggcccggcc catccaccgc cagatcatag gccagcgcct gccaggccgg ccattgcccc
     2701 gccagcgcca gcaccagccc cagccgcaac gccagcccca gcgccgcgcg caagccgccg
     2761 cccgcgccca gcagcaggcg atagcccagc cccgccaccg ccagcgccat cagccccgag
     2821 agcgccgccc cccagcccgt gcccggcccc agcgccgccc agcccgccgc ccccaacgcg
     2881 cgcgcctggc aatcgacaaa gccggtaagc gcgccggcca gcggtgctgc cggatcaaat
     2941 gccgcgcatg cctccatcat cccgcccccc cggccccggc cagcaccggc agccaatcgg
     3001 ccggatccgg ccccacttcg ccgcaaatcg cctcaaaccg gcgcaagctc gcctcccgcc
     3061 ccgacagcac cgccagcaga tccgcctcgc ccgccagatt gagccgcacc accacgcttt
     3121 gcccgccctg cttcaccaga aagcaatggc cattgtcggg caggctgcgc accagcgcgc
     3181 attcatgcgc ggtcaggccg aagccatcgc catagtcctc cgcccgcgcg cgggggttgg
     3241 cggtgaagat ctgcgtcgcc gtctgctcca caatggcgcc ggcgatgcgg ctggccagcg
     3301 catcgccggc attctgggtg acaaagccca gaatgccgcc gcgcttgcgg atggtcttct
     3361 cccagtcgcg gatgtgatgg gcgaagacgg gatcatccag cgccttccag ccctcatcca
     3421 ccacgatgat cgccgggcgg ccatccagcc gctcgtccac ccggtggaag agatacatca
     3481 tcgccggcgt gcgcagcacc ggatcatcga gcaggcgggt catgtccacg cccagcgtcg
     3541 ggcaggcaag gtccagcccg tccgccccat ccggccccgg cggatgatcg aacagccagc
     3601 catgcacgcc atcgccccac cacgggcgca ggcgggtata aagatccccc tcctccgggc
     3661 gctggccccc gcgcagcagc gccaccaggc acgagaggcg ccgcatattc accggcgctg
     3721 cggcattggc cgccaccgct tcctcgatgg cgcgcgcctc ggccgggctc agcggctgct
     3781 gcgcagcgcc cgcaaggcag gccagccaat cggccagaaa gcggcggttg gcggcattgt
     3841 cgggcagcag cagcgggttg aggcccgaag gatggcccgg ccgcagcacc tcgtaacgcc
     3901 cgccgatggc gcgaatgaac agctccgccc cgcgatcctt gtcgaaaaag acgatgcgcg
     3961 gatcaaaccg ccgcgcctgc gccagcagga agttgacgat caccgtcttg cccgagcccg
     4021 acgggccgat caccgtgaaa ttgcccagat ccccgcggtg aaaattgaag tgataggggc
     4081 cggccgccgt cgtctcgaac agggtgacgg catcgcccca gtggttgccc cgcgcctgac
     4141 cctgcggaaa atcatgcccg ctggcaaagg cggcgaaatt ggccgtgccc accagcgcac
     4201 gccgggcgat gaagcggaaa ttgccgggaa actgcgccca gaaggccagt tccagcccgg
     4261 catcctcccg cacggaaacg atgcccagat ccgccagcgc cccttgcacc tcggccaccg
     4321 cgccatccac cgcggcctga tcgacgccgc gcaccagcac gctcatatgg tgctcgccat
     4381 aaagggcgcg gccggcggcc acatcatcct tcgcctgcgc cagctccgcc cgcaaagaca
     4441 gcgcctcgtc atcggccgcg cgcaggcgcc gcagggccag gttcatgcgc gccaggctct
     4501 ccccccgctc gacaaaggcg aagctctggc tcaccaccat ctcgcagggc aggcgcaaaa
     4561 gatcgtccag catgcccggc atcgtctcgc ccggataatc cttgatcgag acgatggcgc
     4621 ccaagctgac ctgcccctgc ccgccggccg ggcccagctc cagcgtatcc gccccgaagc
     4681 tcacccgccg ccagggcaga taatcaccca catcgccctc gggcaggcgc accgggcgca
     4741 gttcggcatt gtacagcgcg gcgagaaatt ccagcgcctc gccgcagggg ccatggggcg
     4801 tgtcatacac cgccaggcgg cgggcgccat aatggcccag cgcggccagc atctgctctg
     4861 ccgctgcatc cagcgcgcgg cattccgccg ccagcagcgc gggcgctgcc ctgcgcccgg
     4921 cccgcctgcg ccacccaccg gccagcctcc ccgccaaccc agccgcctgc ctgcccggcg
     4981 cccggcgcac cagcgtgagg aactgggcat tggcataaag ctgccgcgcc gccagccgcg
     5041 cctgccagcg cgcatccagc ccccggctga aggcatcggc aaaatcgccc cccagatccg
     5101 gccgcacccg ccgccgcagg atatggtgat aaagcgccag ccggggcgag ccgaccgcgc
     5161 gcaacatcgc cgcgcgcaga tccttgcgat agttgatctc ggcagtgtcg gccgtttcgg
     5221 ccatcagccc ttccagggcg atcacctgca tcagccgccc gtcgcgcagc aggatggtgt
     5281 gatcgtcgat ctgcgcggca tagggcaggc gatcgcccgc cggcgcctcg cgggcaaaat
     5341 cgggcataag gcgcgcctgt gccatcatcg ccccctatgg ccgataggaa ttgcaccgcc
     5401 agaagcggcg gttggc
//
LOCUS       DASRYE010000304         9658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_262313,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000304 DASRYE010000000
VERSION     DASRYE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262313"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFF98_15610"
     CDS             complement(<1..776)
                     /locus_tag="VFF98_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU65113.1"
                     /translation="MSRVTEIRYVGYGVKDYAAETAFYTDVWGLERVPSADGWAWFKA
                     PGHGEHHVVRLRPAEENRIDVIALATDSRADVDALHAKVAASGGQIVHPPRDLATPGG
                     GYGFRFFSPDGLQMEISSDVARGPARPVERWEGVPVKISHIVLHSPDHQALVQWFCTV
                     LGFRVSDWLGDFMCFLRCNNAHHRFAILPGPACLNHVAYDMVGVDGMMRGGHRLKQQG
                     FDIRWGPGRHTAGDNTFSYFVTPSGFVAEYTAELEEVDFET"
     gene            complement(802..1917)
                     /locus_tag="VFF98_15615"
     CDS             complement(802..1917)
                     /locus_tag="VFF98_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65114.1"
                     /translation="MNGLDILVIGGGIGGLTSAIALGKAGHHVTVIERDPNWSVYGVG
                     IIQQSNVLAAAHRLGILEAYLAAGVGFDAVEIFTPAGDKVARIPGHPVAEGLPANLGI
                     GRPALHQLLGEAARAAGADIRLGLTATALEETGAGVAARFSDGAHATYALVVGADGVY
                     SATRQMILPDAEVPQFTGQSVWRYNFPRPADLDALQVYNGPTGVGLVPISDRLMYMYA
                     TTPEPDNPRYAQAGLAATMRAKLAHTAPAIQALARQITEDEGVVYRPLEGMMLHGPWH
                     KGRVVLLGDAVHATTPHLGQGAGMAIEDSIVLAEEIARAASPEEAFTAYRARRFERCR
                     FIVEKSLEICHGQIGKGPPVDNARATAEMFAVVSRPI"
     gene            complement(2084..3025)
                     /locus_tag="VFF98_15620"
     CDS             complement(2084..3025)
                     /locus_tag="VFF98_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU65115.1"
                     /translation="MRFRNLDLNLLVVFSGLMETRSVTRTAERLGLTQPAASAALKRL
                     RAYFGDELLVPVGKRMHPTPFAEALFPQIRAGLHTMEQALATPGGFDPATSSRHFRIN
                     ASDYVMVAVLVELAERIAQAAPGIRLEVLLPTEHNVEELNAGRIDLLIAPEQLLAPWH
                     PSEFLYEEEQVVVGWSGNPLLAGGLTPEAFFAAGHVTVALGGQRAATFADGHLMRMGH
                     ARRIEIEVASFASVPWFLQGTQRLAVLHKRLVQRVLPQFDLAFAPMPFPFPRLREMAQ
                     FHEARREDAGLVWLRQQLRLAANPPAQHANPPAQHNA"
     gene            complement(3167..5326)
                     /locus_tag="VFF98_15625"
     CDS             complement(3167..5326)
                     /locus_tag="VFF98_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65116.1"
                     /translation="MSTLAVPAAVRAADAPAAQGDATSLQEIVVTASRSESTAQKTPV
                     ALTAYTGADLAQKGVSNVLALASVDASLNVSTNGGASYVAIRGIASTDVTETGDPSVP
                     ISRDNFFTNRSFSIATSMYDLERVEVLKGPQGTLFGRNSTGGLVQVITKKPGDKLSAD
                     ASLEYGAYNAVKAEAGVSLPLSDKVQLRVAGFYSKHDGYRAIDVLGGRGDDDDTKSGR
                     ITLTFQPFDGLKGLIQYQHDDVHNTGDVAFKTLEGVAYSGQPTDHFANYAPSYNALVG
                     DRVRWEFSYDLPANLTLFYAGGYDHQKWRHSLDASSVTGTGALAGDASSFRQEENPST
                     WNHEIRLATPQNGKLTAQVGYFHFSEHNGNLDSGLYKNSGTYEGQYLIHFLYDVKTRS
                     DGVFGQFGWRPIDPLHITAGMRYTWDSKERTGAAYLRGDIAGFLPSFITTPIVTPGNG
                     NVSEAKPTFHIGADWTFTPHSMAYAKFDTGFKSGGFNSNGSAPSVNYGPETLKAWEMG
                     TKNKFLGGHLMVNADAFYQQYNGYQASQNTGVLSGSASGVFNVGAAEIYGMEAQLAVA
                     SHGWRLDANGTALKTRFTTHSIIVKGDNTTTVDLYGKRLPNAPTFVLTAGLEYGATLA
                     GAKVTPRIEWKHSSSFYFDAFNDADVRQGQYDTFNASVMVKPGHSPFELMFFVRNLTN
                     KSVYASAQENFVTIPNAVDTFEFQPPRTYGVRAAVHF"
     gene            5787..7049
                     /locus_tag="VFF98_15630"
     CDS             5787..7049
                     /locus_tag="VFF98_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65117.1"
                     /translation="MSSAAAAQRPSAPSPAGPAPGAPSPRRQQGVAAGITVVTLGFLP
                     VVAIVSMFPAVPAIIAHFAARDPAAMTKVPSMVSAPGLTVALLALLAGVLVDRFGRRK
                     LLLVATALYGFVGAAPFFLDNLDQIYASRLALGVAEAAILTTLNTLIGDYWDEKGRHL
                     WLTVQGLVGPFLGSAVILASGYLTQIRWNAMFLIYAVGFPAFFACLAFLKEPESDQAA
                     RAMLGMDESAQGFPWRAVSTFGAVTLLGSALYYVFIINGGLVWQEIGVKDPAEIGRLT
                     TIPSLFVMAGAGLFWLTSRFGARAQLFGLFALLGGGLAMIGLIHNPLGMAGAMAVQQT
                     GAGMAVPALIAWAQSHLPFEHRGRGMGVWTACFFFGQFSSPLLVSLVRHFAGTMQGAF
                     LVAGLFGLACAGLVLFLVKGRDAAPNGA"
     gene            7054..8070
                     /locus_tag="VFF98_15635"
     CDS             7054..8070
                     /locus_tag="VFF98_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU65118.1"
                     /translation="MTSKIKRGISLYSFQEELFLGKMNVEDCVAFAAGLGAHGIEVLP
                     EQNMPSFPNITDAQIGQWHEMLARHGAHFTCYDMFLDTKLRKDRLMSDEEQVESILRD
                     LKLCHRLGIKNMRILIFVRPDILEKCVPHAEKYDVHMGVEVHAPWYLDHAWILRTIEV
                     ADRLKTKHLGILPDMGIFMKHYPPAFRARFERQGARPEITQFIVDQHEQKIMCEYTIY
                     EVAVKMGGNKAEIAMAETLRHAPFANPRRIGDFAPYFHHIQAKFYEMTPDCTDPSLAY
                     DEVIPELVKAGWEGTLSSEYEGNRWIQDVEDVDSREQVRRQHVMFERLIAKAEADLAG
                     AVPA"
     gene            8067..8990
                     /locus_tag="VFF98_15640"
     CDS             8067..8990
                     /locus_tag="VFF98_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU65119.1"
                     /translation="MIGRRSLGAALGAALGAALLLAPGLALAQMPMVSRTVLPAPAQP
                     GAIALPGTYAPEAEIWHGDNGLMAVRNVTRPTLTPFLPVGPASGAAVIVAPGGGFLGL
                     AIEKEGWQVARWLANHGVAAFVLKYRVLPTPRDQAEFADKMALVVQGGNPGFAPPDDT
                     PPEALADGVSALRYVRGHAAQFGVDPARVGFMGFSAGGFLTRSLVARGGPDMPAFAAP
                     IYPNMAAITVPPHAPPLFVAMAEDDFLLKRVSGFPLVESYHAAGQPVEFHLFASGGHG
                     FGPGVAGTPTEGWMNLLLHWLRTSGLIKDKP"
     gene            8990..9379
                     /locus_tag="VFF98_15645"
     CDS             8990..9379
                     /locus_tag="VFF98_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6379 domain-containing protein"
                     /protein_id="HZU65120.1"
                     /translation="MFDKFLIDAASVRNTGPQEAPTGFAFEAKLGYYRGLGLSMVEEL
                     NVSIDGEALARAAVTFDEGPGPLTLDEMETAYDRRWPFGTPATIAVAHPGGFPEGAHR
                     LGLQQKLRVSYLPFPSFNTDEKLVERA"
     gene            9460..>9658
                     /locus_tag="VFF98_15650"
     CDS             9460..>9658
                     /locus_tag="VFF98_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65121.1"
                     /translation="MTRLLSRRLAPLLALIALAGPAHAAPRQALVGNTLIYQAVDGSR
                     EIRVYSYADGHYIQWQRDRRAQ"
BASE COUNT         1588 a         3133 c         3249 g         1688 t
ORIGIN      
        1 gtctcgaaat ccacctcttc cagctcggcg gtatattcgg caacgaagcc cgagggggtg
       61 acgaaatagc tgaaggtgtt gtcgccggcg gtgtggcggc cggggcccca gcggatgtcg
      121 aagccctgct gcttcaggcg atggccgccg cgcatcatgc catccacgcc caccatgtcg
      181 taggccacat ggttgaggca ggccgggccc ggcagaatgg cgaagcggtg atgggcgttg
      241 ttgcagcgca ggaagcacat gaaatcgccc agccagtcgc tgaccctgaa gcccagcacg
      301 gtgcagaacc attgcaccag cgcctgatga tcgggcgaat gcagcacgat gtggctgatc
      361 ttcaccggca cgccttccca gcgttccacc gggcgggcgg ggccgcgcgc cacatcgctg
      421 gagatttcca tctgcaggcc atcggggctg aagaagcgga agccatagcc gccgcccggc
      481 gttgccagat cacgcggggg gtgaacgatc tgcccgcccg acgcggccac ctttgcgtgc
      541 agcgcatcaa cgtcggcgcg gctgtctgtg gcaagggcga tcacatcgat gcggttttcc
      601 tcggccgggc gcaggcgcac gacatggtgt tcgccatgcc ccggcgcctt gaaccacgcc
      661 cagccatcgg ccgagggcac ccgttccagc ccccagacat cggtgtaaaa ggcggtttcg
      721 gcggcataat ccttgacgcc atagcccacg tagcggattt cggtcacacg gctcatgaat
      781 ggggtatcct tcgcggaaaa atcagatcgg ccgggagacg acggcaaaca tctcggcggt
      841 ggccctggcg ttatccaccg gggggccctt gccgatctgg ccgtggcaaa tctccaggct
      901 cttctcgacg atgaagcggc agcgctcgaa gcggcgggcg cgataggcgg tgaaggcctc
      961 ttcggggctg gcggcgcggg cgatttcctc ggccagcacg atgctgtcct cgatggccat
     1021 gccggcgccc tgccccagat gcggtgttgt ggcatgcacg gcatcgccca gcagcaccac
     1081 gcggcccttg tgccaggggc cgtgcagcat catcccttcc agcgggcggt agaccacgcc
     1141 ctcatcctcg gtgatctgcc gggccagcgc ctggatggcg ggcgccgtgt gggccagctt
     1201 ggcgcgcatg gtggcggcaa ggcccgcctg cgcatagcgc ggattatccg gctcgggcgt
     1261 ggtggcatac atatacatca gcctgtcgct gatgggcacc aggccaaccc cggtggggcc
     1321 gttataaacc tgcagcgcat ccagatcggc aggccgcggg aaattgtagc gccacaccga
     1381 ctggccggtg aattgcggca cctcggcatc gggcaggatc atctggcggg tggccgaata
     1441 gacgccatcg gcgcccacta ccagcgcata ggtggcgtga gcgccatcgg aaaagcgcgc
     1501 tgccacgccg gcgcccgttt cttccagcgc ggtggcggta aggccgaggc ggatgtcggc
     1561 cccggcggcc ctcgccgcct cgcccagcag ctggtgcagc gccggccggc caatgccgag
     1621 attggccggc agcccttctg ccaccgggtg cccggggatg cgggccacct tgtcgcccgc
     1681 cggggtgaag atctccaccg catcaaagcc gacgccggcg gccagatagg cctcgagaat
     1741 gcccagccgg tgggccgctg ccagcacgtt cgactgctgg atgatgccca cgccatagac
     1801 cgaccagttg ggatcgcgct cgatcacggt gacatggtgg cccgccttgc ccagcgcgat
     1861 ggcgctggtg aggccgccaa tgccgccgcc gatgaccaga atatcgagcc cgttcatcgc
     1921 gccctgcctt gcagcagcgc ggcgcggccg gcccgggcgg gcgtggcatg cggcatcttg
     1981 cattctcccg ttgtgccgcg cgatggcggg ttacgggcgg gttgtaggca gggaaaagag
     2041 atatgtaaaa gcaactgaat tcatgtttta tgatgaattc tgtttatgca ttgtgctgtg
     2101 ctggcggatt ggcgtgctgg gccggcggat tggctgccag ccgcaattgc tggcgcagcc
     2161 agacaaggcc ggcatcctcg cgccgggcct cgtggaactg cgccatctcg cgcaggcggg
     2221 ggaacgggaa aggcatgggc gcaaaggcga gatcgaattg cggcaacacc cgctgcacca
     2281 gccgcttgtg cagcaccgcc agccgctgcg tgccctgcaa aaaccacggc accgaggcaa
     2341 agctggccac ctcgatctcg attcgccggg catggcccat gcgcatcagg tggccgtcgg
     2401 cgaaggtggc ggcgcgctgc ccgcccagcg ccacggtgac atggccggcg gcaaagaacg
     2461 cctctggcgt gaggccgccg gccagcagcg gattgcccga ccagccgacg acaacctgct
     2521 cttcctcata gagaaattcc gatggatgcc agggcgccag caattgctcg ggggcgatca
     2581 gcaggtcgat ccggccggcg ttcagctctt ccacattatg ctctgtgggc agcagcactt
     2641 cgaggcggat gccgggcgcg gcctgggcga ttcgctcggc cagttcgacc agcacggcaa
     2701 ccatcacata atcggaggca ttgatgcgga aatggcggct ggaggtggcc ggatcgaagc
     2761 cgccgggggt ggccagcgcc tgttccatag tgtgcagccc ggcgcggatc tgcggaaaca
     2821 gcgcctcggc aaagggggtg ggatgcatgc gcttgcccac cggcaccagc agttcatcgc
     2881 cgaaataggc ccgcagccgc ttgagcgcgg cactggcggc cggctgggtg agcccgagtc
     2941 gctctgccgt gcgggtgacg ctgcgggttt ccatcaggcc ggaaaacacc accagcaggt
     3001 tgagatcgag gttcctgaag cgcattaaag cataaatcct gagtggataa tgccggcatt
     3061 agataaattc agcgcatgat aaggcaaggg cggatgcagg ccttgccccg catccgcccc
     3121 gacgtttgcc tgccaggcag cagggccgcg ccctgccgcc cgccggtcag aagtgcacgg
     3181 cagcgcgcac gccataggtg cgcggcggct ggaattcgaa cgtgtccacc gcgttgggga
     3241 tggtcacgaa attctcctgc gcgctggcat agaccgactt gttggtgagg ttgcgcacga
     3301 agaacatcag ctcgaacggg ctgtggccgg gcttcaccat caccgaggcg ttgaacgtgt
     3361 cgtactggcc ctggcgcaca tcggcatcgt tgaaagcgtc gaaatagaag ctggaggaat
     3421 gcttccattc gatgcgcggc gtgaccttgg cgccggccag cgtggcgcca tattccagcc
     3481 ccgcggtcag cacgaaggtg ggggcgttgg gcaggcgctt gccatagaga tcgacggtgg
     3541 tggtgttatc gcccttgacg atgatgctgt gggtggtgaa gcgggtcttc agcgcggtgc
     3601 cattggcatc gagccgccag ccgtggctgg ccaccgccag ctgcgcttcc atgccataga
     3661 tctcggccgc gccgacattg aacacgcccg atgccgaacc cgacagcacg ccggtgttct
     3721 gcgaggcctg atagccgtta tactgctgat agaaggcatc ggcattgacc atcagatggc
     3781 cgcccagaaa cttgttcttg gtgcccattt cccaggcctt cagcgtctcg ggcccgtaat
     3841 tcacggaagg agccgagccg ttggagttga agccgcccga tttgaagccg gtgtcgaact
     3901 tggcataggc catcgaatgg ggggtgaagg tccagtcggc gccgatgtgg aaggtgggct
     3961 tggcctcgct cacattgcca ttgccggggg tgacgatcgg cgtggtgatg aaggagggca
     4021 ggaagccggc gatatcgccg cgcagatagg ccgcgcccgt gcgctccttg ctgtcccagg
     4081 tgtagcgcat gccggcggtg atgtgcagcg gatcgatcgg gcgccagccg aactggccga
     4141 acacgccatc cgagcgcgtc ttcacatcat agaggaagtg gatgagatac tggccctcgt
     4201 aggtgccgct gttcttgtag aggccgctgt cgagattgcc attgtgctcg ctgaagtgga
     4261 aatagcccac ttgcgcggtc agcttgccgt tctggggggt ggcgaggcgg atctcgtggt
     4321 tccaggtgct ggggttctct tcctggcgga agctggaggc atcgccggcc agcgcgccgg
     4381 tgccggtgac agagctggca tcaagcgagt ggcgccactt ctggtgatca tagccgccgg
     4441 catagaacag ggtgaggttg gcgggcagat catagctgaa ttcccagcgc acccgatcgc
     4501 ccaccagcgc gttgtagctg ggcgcgtaat tggcgaaatg atcggtcggc tggccggaat
     4561 aggccacgcc ttccagcgtc ttgaaggcca catcgccggt gttgtgcaca tcgtcgtgct
     4621 gatactggat gaggcccttc agcccgtcga acggctggaa ggtgagcgtg atgcggcccg
     4681 acttggtgtc atcgtcatcg cctctgccgc ccagcacgtc gatcgcgcga tagccatcat
     4741 gcttgctgta gaagccggcc acgcgcagct gcaccttgtc cgacagcggc aggctgacgc
     4801 cggcctcggc cttcacggcg ttataggcgc catattccag cgaggcatcg gccgagagct
     4861 tgtcgcccgg cttcttggtg atgacctgca ccaggccgcc ggtggaattg cggccgaaca
     4921 gcgtgccctg tgggcccttc agcacttcca cgcgttccag atcatacatc gaggtggcga
     4981 tcgagaagga gcggttggtg aagaaattgt cgcggctgat cggcaccgag gggtcgccgg
     5041 tctcggtcac gtcggtggag gcgatgccgc ggatggcgac ataggaggcg ccgccattgg
     5101 tggagacatt gaggctggca tcgaccgagg ccagcgccag cacgttgctc acgcccttct
     5161 gggccagatc ggcgccggtg taggcggtca gcgccaccgg cgtcttctgc gcggtgcttt
     5221 cgctgcggct ggcggtgacg acgatttcct gcaggctggt ggcatccccc tgcgcggcgg
     5281 gtgcatcggc ggcgcgcacg gctgccggca ccgccagagt gctcacgcca gcgcaaaggg
     5341 ctgcgtaaaa atgacgcttc atggcaatcc tccccaatca tggtccgcct tggcggcttc
     5401 gaccaaatac gaaatcagaa tatgtgttct ggtaaaggcg agcagcatgg cttgtcaaca
     5461 cccgctgaca gagaggccgc ccccccccgc agttgcgaca catccgcaac cgcatcgtgg
     5521 cattcgcgca acagctgccg ccggcacgac cccgcaagag agcgccattt tgctgcagtt
     5581 gcgcagaaaa tccggtgtgc cacggcaggc cgccggcggg actggccggc agccccggcg
     5641 caaggggcct tgacatcggc cgcgcgcgct gtgatgcata tttcagaata aaagttctaa
     5701 ttgactgccg gcaagatccg gtccgtcaac ggccggtgcg ccacaggtgc cggccgccgc
     5761 atccattgcc tggggagagc cagctcatgt catccgccgc cgccgcccag cggccatccg
     5821 ccccatcgcc cgccggcccg gcgccgggcg cgccgtcgcc gcgccgccag cagggcgtgg
     5881 ccgccggcat caccgttgtc acgctgggct tcctgccggt ggtggcgatc gtctcgatgt
     5941 ttccggcggt gccggcgatc atcgcccatt tcgccgcgcg cgatccggcc gccatgacca
     6001 aggtgccctc gatggtgtcg gcgccgggcc tcacggtcgc gctgctggcg ctgctggcgg
     6061 gcgtgctcgt ggacaggttc ggccgccgca agctgctgct ggtggccacc gcgctttatg
     6121 gctttgtcgg cgctgccccc ttcttcctcg acaatctcga ccagatctat gcctcgcggc
     6181 tggcgctagg ggtggcagaa gcggccatcc tcaccacgct caacacgctg atcggcgatt
     6241 attgggacga gaaaggccgc cacctgtggc tgaccgtgca gggcctggtg ggcccgtttc
     6301 tgggcagcgc ggtgatcctc gcctccggct atctcacgca gatccgctgg aacgccatgt
     6361 tcctcatcta tgccgtgggc tttcccgcct ttttcgcctg ccttgccttc ctgaaagagc
     6421 ccgagagcga ccaggccgcg cgcgccatgc tgggcatgga tgaaagcgcg cagggctttc
     6481 cctggcgcgc ggtgagcacc tttggcgcgg tgacgctgct gggcagcgcg ctctattacg
     6541 tcttcatcat caatggcggg ctggtgtggc aggagatcgg cgtgaaggac ccggccgaga
     6601 tcggccgcct caccaccatt cccagcctgt tcgtgatggc gggcgccggc ctgttctggc
     6661 tgaccagccg attcggcgcg cgcgcgcagc tgtttggcct gtttgccctg ctgggcggcg
     6721 ggctggcgat gatcggcctc atccacaatc cgctgggcat ggccggcgcc atggccgtgc
     6781 agcagacagg cgccggcatg gccgtgccgg cgctgatcgc ctgggcgcaa agccacctgc
     6841 ctttcgaaca tcgcgggcgc ggcatgggcg tgtggacggc ctgcttcttc ttcggccagt
     6901 tttcctcgcc gctgctggtc agcctggtgc gccattttgc cggcaccatg cagggcgcct
     6961 tcctggtggc gggcctgttt ggcctggcct gcgccgggct tgtccttttc ctcgtcaagg
     7021 ggcgcgatgc cgcccctaac ggagcctgac accatgacat cgaagatcaa gcggggcatc
     7081 agcctctaca gctttcagga ggaactgttc ctcggcaaga tgaacgtgga ggactgcgtg
     7141 gcctttgccg ccggccttgg cgcccatggc atagaagtgc tgcccgagca gaacatgccc
     7201 tccttcccca acatcaccga tgcccagatc ggccagtggc acgagatgct ggcccgccat
     7261 ggcgcccatt tcacctgcta cgacatgttc ctcgacacca agctgcgcaa ggaccggctg
     7321 atgagcgacg aggagcaggt ggaaagcatc ctgcgcgacc tgaagctgtg ccaccggctg
     7381 ggcatcaaaa acatgcgcat cctcatcttc gtgcggcccg acatcctcga gaaatgcgtg
     7441 ccccatgccg agaagtatga cgtgcacatg ggcgtggaag tgcatgcgcc ctggtatctc
     7501 gaccatgcct ggatcctgcg caccatcgag gtggccgacc ggctgaagac gaagcatctg
     7561 ggcattctgc ccgatatggg catcttcatg aagcattatc cgccggcgtt ccgcgcccgt
     7621 ttcgagcggc agggggcaag gccggagatc acccaattca tcgtcgatca gcacgaacag
     7681 aagatcatgt gcgaatacac gatctatgag gtggcggtga agatgggcgg caacaaggcg
     7741 gaaatcgcca tggccgaaac gctgcgccac gccccctttg ccaaccccag gcggattggc
     7801 gatttcgcgc cctatttcca ccatattcag gcgaagttct acgagatgac gccggattgc
     7861 accgacccct cgctggctta cgacgaggtg atccccgagc tggtgaaggc cggctgggaa
     7921 ggcacgctca gttccgaata tgagggcaac cgctggatcc aggacgtgga ggacgtggac
     7981 agccgcgagc aggtgcgccg ccagcatgtg atgttcgagc ggctgatcgc caaggcagag
     8041 gctgatctgg ccggcgcggt gccggcatga tcggccggcg ctcccttggg gcggcgcttg
     8101 gtgcggcgct gggcgcggcg ctgctgctgg cgccaggcct ggcgctggcg cagatgccga
     8161 tggtctcgcg cacggtgctg cccgcgccgg cccagcccgg cgccatcgcc ctgcccggca
     8221 cctatgcccc cgaggccgag atctggcatg gcgacaatgg cctgatggcg gtgcgcaatg
     8281 tcacgcggcc cacgctcacg ccgttcctgc cggtggggcc ggccagcggc gcggcggtga
     8341 tcgtggcgcc gggcggcggc ttcctcggcc tggccatcga gaaggagggc tggcaggtgg
     8401 cgcgctggct ggccaaccat ggcgtggccg ccttcgtgct gaaatatcgc gtgctgccca
     8461 cgccccgcga tcaggccgaa tttgccgaca agatggcgct ggtggtgcaa ggcggcaacc
     8521 ccggctttgc cccgcccgac gacacgcccc ccgaagctct tgccgatggc gtgagcgcgc
     8581 tgcgctatgt gcgcggccat gcggcgcaat ttggcgtgga tccggcgcgg gtgggcttca
     8641 tgggcttttc cgccggcggc ttcctcaccc gcagcctggt ggcacgcggc gggcccgaca
     8701 tgcccgcctt tgccgccccc atctatccca acatggccgc catcaccgtg ccgccccatg
     8761 ccccgccgct gttcgtggcc atggccgagg atgatttcct gctgaagcgg gtgagcggct
     8821 ttccgctggt ggaaagctat catgccgccg gccagcctgt ggaattccat ctgtttgcca
     8881 gcggcggcca cggctttggc cccggcgttg ccggcacccc caccgaaggc tggatgaacc
     8941 tgctgcttca ctggctgcgc accagcggcc tgatcaagga caagccctga tgttcgacaa
     9001 attcctgatt gatgccgcct ctgtgcgcaa caccgggccg caggaagcgc ccaccggctt
     9061 cgccttcgag gccaagctgg gctattaccg gggccttggc ctgtcgatgg tggaggagct
     9121 gaacgtctcg atcgacggcg aggcgctggc gcgcgcggcc gtcactttcg acgaggggcc
     9181 cgggcccctg acgctcgacg agatggagac cgcctacgac cggcgctggc ccttcggcac
     9241 gccggcaacg attgccgtgg cccaccccgg cggttttccc gagggcgcgc accggctggg
     9301 gctgcagcag aagctgcggg tgtcctacct gcccttcccc tcgttcaaca ccgacgagaa
     9361 gctggtggaa cgggcctgag cccttatccc tgaaaggggg cagcggcgcg gcgccgatgc
     9421 ccccttccct cgccccgctt ctgccgatac atccatgcca tgacgcgcct gctctcccgc
     9481 cgccttgcgc ccctgcttgc cctgatcgcg ctggcaggcc cggcccatgc cgcgccccgg
     9541 caggcgctgg tgggcaacac gctgatctat caggcggtgg atggcagccg cgagatccgc
     9601 gtctattcct atgccgacgg gcattacatc cagtggcagc gcgaccggcg ggcgcagg
//
LOCUS       DASRYE010000305         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_65436,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000305 DASRYE010000000
VERSION     DASRYE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65436"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1446)
                     /locus_tag="VFF98_15655"
     CDS             complement(208..1446)
                     /locus_tag="VFF98_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HZU65122.1"
                     /translation="MSRVLHIFDHSLPLHSGYTFRSRAILGAQQALGLEVRGVTGLRH
                     YAPGPDVEEAEGFTFLRTRGSTGGPPIWREGREVMALAHAATCAARDWGADVLHAHSP
                     ALCGLAGLIAARRLGLPLVYEIRAFWEDAAVGNGTGRAGDLKYRLTRALENHVVRRAD
                     AVITICAGLKQDLVARGIAPGRITIMPNGVDMGLFGTPAPRDGALARAIGLEDAAGPC
                     PTIGFIGSFYDYEGLDDLIAAMPALVAQQPRARLLLVGGGPREEALRAQAAASPVAGA
                     IRFIGRVPHHEVERYYALADIMAYPRKQSRLTDLVTPLKPLEAMAQGKLVAASDVGGH
                     RELIPEGTGTLFTPDSPAACAAALARLLAAQGQWAGIRSRARAHVAQNHDWARNAARY
                     LPVYQRLSNKVDSHLRVVPA"
     gene            1531..1716
                     /locus_tag="VFF98_15660"
     CDS             1531..1716
                     /locus_tag="VFF98_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65123.1"
                     /translation="MIDIFALLVTHALLALAALRVVANAALDRDEADAPEAPAAEAPP
                     PPAPAGRMRPLRAPPVE"
     gene            1721..3109
                     /locus_tag="VFF98_15665"
     CDS             1721..3109
                     /locus_tag="VFF98_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5935 domain-containing protein"
                     /protein_id="HZU65124.1"
                     /translation="MLNLFLTGFVGLLIALGLRRPFIWVLLYLYVDILAPQKVSLGFL
                     SSVPISLIAFVLAGSSWLFLDDKRDSRFTFRQVLLVLLLVYCGFTTMSADFPDAAAEK
                     WAWVWKALVFAIFLPLTLRTRLRIEASVLVMGLSAGAIVIGGALKTLATGGGYNELKL
                     FVNDNTGLYEGSIISCVSVAIIPLVLWLANHGSIFPPDRRARFYAYAQCFACLLIPVG
                     TQARTGLLCLGLLGVMALRSVKRPMLFVAGAAVALVLAIPFLPKTYTDRMGTIQNHEA
                     DESASTRIAVWTWTLGYVQDHPLGGGFAAYLQDKITVQTRSVSGDGNTAAVSVDSMQD
                     KARAFHSSYFEMLGEQGWPGLFLWLTLQISGVVQMEMLNRRWRRRYREAPVPANSWQA
                     PLAMALQQAQLVYMCGSLFVGIAFQPFILMLVGLQCGLWSYLGRIEAAERSARPAGRP
                     APRLLRTGPASG"
     gene            3154..3609
                     /locus_tag="VFF98_15670"
     CDS             3154..3609
                     /locus_tag="VFF98_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZU65125.1"
                     /translation="MDKQLQVVDLVPLVGTVLGISDWMVVDQPLIDAFAQVTGDHEFL
                     HVDPVAAAATPLGGTIAHGLLTLSLLPALFNASCPRPACRMTVNYGYDAVRFLSPVPV
                     GSRLRGAFRLVELVERKPGEWRQSVAASVEIEGAERPALVATWLCQHFA"
     gene            3620..>3907
                     /locus_tag="VFF98_15675"
     CDS             3620..>3907
                     /locus_tag="VFF98_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZU65126.1"
                     /translation="MREAVIVSAARTPIGRAFKGAYADLHPVQLGAHAAAAAVARAGL
                     DGAEVEEVIWGAAVTQGGQGPNLGRLVALSAGLPVSTAGMVVERQCASGLMA"
BASE COUNT          536 a         1350 c         1361 g          660 t
ORIGIN      
        1 aggtcgatcc cgctgttcac cacggcctgc tccagcagcc cggtgcgaat cgccagggtg
       61 gacaggccga acagcgccgc aaaccacagc gccagcgccg gggcaaacag ccgatgcgcg
      121 gcaacactgc gcttttcctg catatctgct ctgtcgtgcc ggctcacatc agcccccgtt
      181 gtctggcgcc ggggggccgg ccagggatca ggcaggcacc acgcgcaagt gcgagtctac
      241 cttgtttgac agccgttggt aaacgggaag ataacgcgcc gcattgcgcg cccaatcgtg
      301 attttgtgcc acatgggcac gcgcgcggct gcgaatgccc gcccattgcc cctgcgcggc
      361 cagcaggcgg gcgagggcgg cggcacaggc cgcgggcgaa tcgggggtga acagcgtgcc
      421 ggtgccctcg gggatcagct cgcggtggcc gcccacgtcg ctggccgcca ccagcttgcc
      481 ctgggccatt gcctccagcg gcttcagcgg cgtcaccaga tcggtcagcc ggctttgctt
      541 gcgcggatag gccatgatgt cggccagcgc gtaatagcgt tccacctcgt ggtggggcac
      601 gcggccgatg aagcggatgg cgccggccac cgggctggcg gcggcctgcg cgcgcagcgc
      661 ttcctcgcgc gggccgccgc ccaccagcag caggcgcgcg cgcggctgtt gcgccaccag
      721 cgccggcatc gcggcgatca ggtcgtccag cccctcgtaa tcgtagaaac tgccgatgaa
      781 gccgatggtg gggcaggggc ctgcggcatc ttcaaggcca atggcgcggg ccagcgcgcc
      841 atcgcgcggg gcgggcgtgc cgaacaggcc catatccacc ccgttgggca tgatggtgat
      901 ccgcccgggg gcgatgccgc gcgccaccag atcctgcttc agcccggcgc agatggtgat
      961 caccgcatcg gccctgcgca ccacatggtt ttccagcgcc cgcgtcagcc ggtatttgag
     1021 atcgcccgcc cggccggtgc cattgcccac cgccgcatcc tcccagaagg cgcggatctc
     1081 gtagaccagc ggcaggccca gccgccgcgc cgcgatcagc cccgccagcc cgcacagcgc
     1141 cggcgaatgg gcatgcagca catcggcgcc ccaatcgcgc gcggcgcagg tggcggcatg
     1201 ggccagcgcc atcacctccc ggccctcgcg ccagatcggc gggccgccgg tgctcccgcg
     1261 cgtgcgcagg aaagtgaagc cctcggcctc ttccacatcg gggccggggg catagtggcg
     1321 cagccccgtc accccgcgca cttccagccc cagcgcctgc tgcgcgccca gaatcgcccg
     1381 gctgcggaag gtgtagccac tgtgcagcgg cagcgaatga tcgaagatat ggagaacccg
     1441 gctcatgggc gcttctcctg ccacggcaag gtttaacgcg ccgtcaaccg ccgcgcgatt
     1501 aagccatggc ggccacaagg aaccgcccgc ttgatcgaca ttttcgcgct gcttgtcacc
     1561 catgccctgc tggcgctggc cgccttgcgg gtggtggcca atgccgcgct ggatcgcgac
     1621 gaggccgacg cccctgaagc ccccgctgcc gaagcgccgc cgccccccgc gccggccggg
     1681 cggatgcgcc ccttgcgcgc gccgccggtg gaatagggcg atgctcaacc tcttcctcac
     1741 cggctttgtc ggcctgctga tcgcgctggg gctcaggcgc ccgttcatct gggtgctgct
     1801 ctacctctat gtcgatattc tcgcgccgca gaaggtgtcg ctgggctttc tctcctcggt
     1861 gccgatctcg ctgatcgctt tcgtgctggc cggctccagc tggctgttcc tggatgacaa
     1921 gcgcgattcg cgcttcactt tccgccaggt gctgctggtg ctgctgctgg tctattgcgg
     1981 cttcaccacc atgtcggccg atttccccga tgccgcggcc gagaaatggg cctgggtgtg
     2041 gaaggcgctg gtgtttgcca tcttcctgcc gctcaccttg cgcacgcgcc tccggatcga
     2101 ggcctcggtg ctggtgatgg ggctgtcggc gggcgccatt gtcatcggcg gcgcgctgaa
     2161 gaccctggcg acgggcggcg gctataacga actcaagctc ttcgtgaacg acaataccgg
     2221 gctttacgaa ggctcgatca tttcttgcgt gtcggtggcg atcatcccgc tggtgctgtg
     2281 gctggccaac cacggcagca tcttcccgcc agaccggcgc gcgcgcttct atgcctatgc
     2341 ccagtgcttt gcctgcctgc tgatccctgt cggcacgcag gcgcgcacgg gccttttgtg
     2401 cctgggcctg ctgggggtga tggcgctgcg cagcgtgaag cggccgatgc tgtttgtggc
     2461 aggggcggcc gtagcgctgg tgctggcaat ccccttcctg cccaaaacct ataccgatcg
     2521 catgggcacc atccagaacc acgaggccga cgaatcggcc tccacccgca ttgccgtgtg
     2581 gacctggacc ctgggctatg tgcaggatca tccgctgggc ggcggctttg ccgcctatct
     2641 gcaggacaag atcaccgtgc agacgcgctc tgtctcgggc gatggcaaca ccgccgccgt
     2701 ctcggtggac agcatgcagg acaaggcccg cgccttccac tcctcctatt tcgagatgct
     2761 gggcgagcag ggctggccgg gcctgttcct gtggctgacg ctgcagatct cgggcgtggt
     2821 gcagatggag atgctgaacc gccgctggcg ccgccgctac cgcgaggcgc cggtgcccgc
     2881 caacagctgg caggcgccgc tggccatggc gctgcagcag gcgcagctgg tctatatgtg
     2941 cggctcgctg tttgtcggca ttgccttcca gcccttcatc ctgatgctgg tggggctgca
     3001 atgcgggctg tggagctatc tcggccggat cgaggcggca gagcgctcgg cccggcccgc
     3061 cggcaggccc gccccccgcc tgctgcgcac cggccctgcc agcggatagc cgtcgcttgc
     3121 catggggccc cctgcgggcc taaggccggc gccatggaca agcagttgca ggtggtggat
     3181 ctggtgccgc tggtcggcac ggtgctcggc atatccgact ggatggtggt ggatcagccg
     3241 ctgatcgatg ccttcgcgca agtgacgggc gaccatgaat ttctccatgt cgatccggtg
     3301 gcggccgcgg ccacgccgct gggcggcacc attgcccacg ggctgctcac cctgtcgctg
     3361 ctgccggcgc tgttcaacgc cagctgcccg cgcccggcct gccggatgac ggtgaactat
     3421 ggctatgacg cggtgcgctt cctcagcccg gtgccggtgg gcagccgcct gcgcggcgcc
     3481 ttccgcctgg tggagctggt ggagcgcaag cccggcgaat ggcgccagag cgtggccgcg
     3541 agcgtggaga tcgagggggc cgaacgcccc gcgctggtgg cgacatggct gtgccagcat
     3601 tttgcctgag gcgccgctga tgcgcgaggc ggtgatcgtc tctgccgcgc gcacgccgat
     3661 cggccgggcc ttcaaggggg cctatgccga tctgcatccg gtgcagctgg gcgcccatgc
     3721 ggcagcggcg gccgtggccc gcgccgggct cgatggcgcc gaggtggaag aggtgatctg
     3781 gggcgcggcg gttacgcaag gggggcaggg gcccaatctg gggcggctgg tggcgctgtc
     3841 ggcggggctg ccggtcagca cggcgggcat ggtggtggaa cggcaatgcg cctccgggct
     3901 gatggcg
//
LOCUS       DASRYE010000306         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_262484,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000306 DASRYE010000000
VERSION     DASRYE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262484"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VFF98_15680"
     CDS             complement(<1..1433)
                     /locus_tag="VFF98_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65127.1"
                     /translation="MLWPAAALAGPLPQAAIIIPAQPLEQALMELARQGGGNILFQPG
                     SMARLVSHPVHARQPAAALRQMIAGLPVRITRQHRGMAILPGLPGAALPARPPLRAPG
                     GPARPQPAPLADQPVILVTAAPGASAFGSERRRPPRADDLLLAPALHPTDRNLAEAFG
                     HAPGVLLLATNLQGDLGGIDRAGRAEGQFLAIRGLSGAHAAVRVDGVDLPESLPYGRD
                     AELGMLSGLLFDKARIATTPGPQEAGDATSALVDLATPSAFAAGQGGLHLSASTDLDS
                     QAIAYRQPATSWQAAARYSRRFGAGQDWGLALGATVSRRVFATVEQTYQSGTVELARV
                     TAAGTSPAGINPASNLLLTDLNLEFTRGYTLATAFSGALEWRGASHFLALRALYATSH
                     TEQDVYQLGFQGGNSAQDETLASLGNGISQITSTTARLHYWYQTNPQSSRLGLVQLAA
                     GGESPAGTGTLGWQGRLYRAEGITARPR"
     gene            complement(1513..2487)
                     /locus_tag="VFF98_15685"
     CDS             complement(1513..2487)
                     /locus_tag="VFF98_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZU65128.1"
                     /translation="MSAPGPDLAPLTPAEEQRVWQDAAGWVARAHEGGLSAAQQALFL
                     AWLRARPEHAAAADLASQAWATAASASRPPAAPAPAPAPPLAAHRWRPALALALGLVA
                     MVGGGLGWRAGCHSARYATAPGERATHQLADGTRLWLAPGSSLVVEITPLARRVTLAK
                     GEAAFDVAHQWRPFRVRAQGLEAVDQGTLFTVRSRQPGHVQVVLARGAVDVRDRASGA
                     LLASLRPGEALNREGTALQVAQVDAEAALGWRSGRLTFERMALGEALARFAEQGAAPV
                     RLADPRLAALPVSGAYDVADMGSFLAGIAIVLPLRIRQDSKGYVIEER"
     gene            complement(2484..3104)
                     /locus_tag="VFF98_15690"
     CDS             complement(2484..3104)
                     /locus_tag="VFF98_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZU65129.1"
                     /translation="MITAAPPSPLPQPDRPPPAEPATQAPPASRTLPDPRELESHRPT
                     LRQYFTRRIRDAGEAEDYVQDVYLRVLAAGQEPKKIHNLRGFLLRAASSVWIDRHRRA
                     KTRIEQDGCTLTEDITANLADTAAASPERTAIARDALRRLSAEIDTLPPTAREAFRLV
                     RIDGLSHKEAARRLGLEPRQVAHHVERSLARLGQAMLETSMPENAP"
     gene            3291..>3675
                     /locus_tag="VFF98_15695"
     CDS             3291..>3675
                     /locus_tag="VFF98_15695"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU65130.1"
                     /translation="MQFSSRIGWLAGVASLALATGAHAAGAGVTAEAGVAEASDAQTG
                     DAPADSIVVLAKRADAASLEFRSALPLSALSQEDLEHTAVHNVAEALGLLPGVNVMNT
                     GSSYFGGVDGASRGEGMFVAIRGLNS"
BASE COUNT          494 a         1252 c         1395 g          534 t
ORIGIN      
        1 cgggggcgcg cggtgatccc ctcggcccgg tagagccgcc cctgccagcc cagcgtgccg
       61 gtgcccgccg ggctttcgcc gccggccgcc agctggacaa ggcccagccg gctgctttgc
      121 gggttggttt gataccaata atgcagccgg gcggtggtgc tggtgatctg gctgatgcca
      181 ttgcccaggc tggccagggt ttcatcctgc gccgaattgc cgccctgaaa gcccagctga
      241 taaacatcct gctcggtgtg gctggtggca taaagcgccc gcaaggcgag aaaatggctg
      301 gcgccgcgcc attccagcgc gccgctgaag gcggtggcga gcgtatagcc gcgggtgaat
      361 tcaaggttga gatcggtgag cagcaggttg ctggccgggt tgatgccggc agggctggtg
      421 ccggcagcgg tcacccgcgc cagttcgacc gtgccggact gataggtctg ttccaccgtg
      481 gcgaacaccc gccgcgagac agtggccccc agcgccagcc cccaatcctg cccggcgcca
      541 aagcggcggc tgtagcgggc ggcggcctgc cagctggtgg cgggctggcg ataggcgatc
      601 gcctggctgt cgagatcggt gctggccgaa aggtgcaggc cgccttggcc ggcggcaaag
      661 gccgaagggg tggccagatc gaccagcgcc gaggtggcat cgccggcctc ctgcgggccg
      721 ggggtggtgg cgatgcgggc cttgtcgaac agcaggcccg agagcatgcc cagctccgca
      781 tcgcggccat agggcaggct ttcgggcagg tccacgccat ccacccgcac cgccgcatgg
      841 gcgccgctga ggccgcggat ggccagaaac tgcccttcgg cccggccggc gcggtcgatc
      901 ccgcccagat cgccctgcag gttggtggcc agcagcagca cgcccggcgc atggccaaag
      961 gcttcggcca gattgcggtc tgtggggtgg agggcagggg ccagcagcag atcatcggcg
     1021 cggggcgggc ggcggcgctc gctgccaaag gcgctggcgc ccggcgctgc ggtcaccagg
     1081 atgaccggct gatcggccag cggcgcgggc tgcggccggg cggggccgcc gggggcgcgc
     1141 agcggcggcc gggcgggcag ggcggcgccc ggcaggcccg gcaggattgc catgccgcgg
     1201 tgctggcggg tgatgcgcac cggcaggccg gcgatcatct ggcgcagcgc ggcggccggc
     1261 tggcgggcat gcaccgggtg cgagaccagc ctggccatgc tgcccggctg gaagaggata
     1321 ttgccgccgc cttgccgggc cagctccatc agcgcctgtt ccagcggctg ggcggggatg
     1381 atgatggccg cttgcggcag ggggccggcc aatgcggccg ccggccacag catcagccac
     1441 ggcatcgacc atcgggccgc gcgccgcctg ccatcccgga tcatgcgaat gccacgccca
     1501 tgcgatgcag cgctagcgct cttcgatgac atatcctttg ctgtcctggc ggatgcgcag
     1561 gggcagcacg atggcaatcc cggccaggaa ggagcccatg tcggccacat cataggcgcc
     1621 gctgacaggc agcgcggcca ggcgcggatc ggccaggcgc acgggcgccg cgccctgttc
     1681 ggcaaagcgg gccagcgcct cgcccagcgc catgcgctcg aaggtgaggc ggccgctgcg
     1741 ccagcccagc gcggcctcgg catccacctg cgccacttgc agcgcggtgc cctcgcggtt
     1801 cagcgcctcg ccggggcgca ggctggccag cagggcgccg ctggccctgt cgcgcacatc
     1861 caccgcgccg cgcgccagca ccacctgcac atgccccggc tggcggctgc gcaccgtgaa
     1921 caaggtgccc tgatcgaccg cttccagccc ctgcgcgcgg acccggaagg gccgccactg
     1981 atgcgccaca tcaaaggcgg cctcgccctt tgccagggtc acgcggcggg ccagcggggt
     2041 gatctccacc accaggctgc tgcccggggc cagccacagg cgagtgccat cggccagctg
     2101 gtgggtcgct ctttcgccgg gggcggtggc atagcgggcg ctgtggcagc cggcccgcca
     2161 gccaagcccg ccaccaacca tggcgacaag gcccagcgcc agcgccagcg ccgggcgcca
     2221 gcggtgggcc gccagcggcg gggcgggggc gggggcaggg gccgctggcg gtcggctggc
     2281 gctggcagct gtggcccagg cctggctggc cagatcggcg gcggcggcat gttcgggccg
     2341 ggcgcgcagc caggcgagga acagcgcctg ctgcgcggca gacaggccgc cctcatgcgc
     2401 gcgcgccacc cagccggcgg catcctgcca cacgcgctgt tcctcggccg gggtcagcgg
     2461 ggcaaggtcg gggccggggg cgctcatggc gcgttctccg gcatggatgt ctccagcatg
     2521 gcctggccca gccgcgccag gctgcgctcc acatgatggg ccacctgccg gggttccagc
     2581 cccagcctgc gggcggcttc cttgtggctc agcccgtcta tgcgcaccag ccggaaggcc
     2641 tcgcgcgcgg tgggcggcag cgtgtcgatc tcggccgaca ggcggcgcag cgcatcgcgt
     2701 gcgatggcgg tgcgctcggg gctggcggcg gcggtgtcgg ccagattcgc ggtgatatcc
     2761 tcggtcagtg tacagccatc ctgctcgata cgtgtcttgg cccggcggtg ccggtctatc
     2821 catacgctag acgcagcgcg cagcaaaaat ccgcgcaggt tgtggatttt tttgggttcc
     2881 tgcccggccg ccagcacccg cagatacaca tcctgcacat aatcctcggc ctcgcccgca
     2941 tcgcggatgc ggcgggtgaa atactggcgc agcgtggggc ggtgcgattc cagttcgcgc
     3001 gggtcgggca gcgtgcggct ggccggtggg gcctgggtgg caggctctgc cggcgggggc
     3061 cggtcgggct ggggaagggg ggatggtggg gcggcggtga tcatggcgct ggccggccgc
     3121 tcctggctct cgggtggtct ttccggcggg gaaagcggct gcctttaagg gcccgatgat
     3181 gaccgcatga tgacataaat ttttgtttcg tgagggccgg aatcggccgc ccgcttgcgt
     3241 ctctggggga agaggcaggc gatcacgcgc ctcgccaaca ggaggcacag atgcaatttt
     3301 cctcgcgtat tggctggctg gccggggttg ccagcctggc tcttgccacg ggcgcccatg
     3361 ctgccggcgc cggcgtcacc gccgaggccg gcgttgccga agccagcgat gcccagactg
     3421 gcgatgcgcc tgccgacagc attgtcgtgc tggccaagcg ggccgatgcc gcctcgctgg
     3481 aattccgctc ggcgctgccg ctctcggcgc tctcgcagga ggatctggaa cacaccgcgg
     3541 tgcataatgt ggccgaggcg ctgggcctgc tgccgggcgt caatgtgatg aacaccggct
     3601 cgtcctattt cggcggcgtt gatggcgcct cgcgcggcga gggcatgttc gttgccatcc
     3661 gcgggctcaa ttcgg
//
LOCUS       DASRYE010000307         9415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_47210,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000307 DASRYE010000000
VERSION     DASRYE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47210"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFF98_15700"
     CDS             complement(<1..893)
                     /locus_tag="VFF98_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU65131.1"
                     /translation="MTTAATLIDWDALSQRLSPIEVSTAEKVIKRKSRDYYWFSPVLK
                     AQLEEVRADAIVTPASAGEVSQVVRFCVENRLPLTLRGGGTGNYGQAMPLKGGVVMDM
                     TRLTAICHIGDGVVRAEAGAKMYDIEQAVNAAGYELRFFPSTWKSATIGGFVAGGSTG
                     CGAIGFGTLHSGDNVRAAKVLTAEESPRILELTGRDAQAVVHAYGTNGVILEVELALA
                     PRRAWHDRIVAFDDLPTTLAFAAALGADGAIPKRQLTIYGATTASLIRPVADLDTGAC
                     PLVLTMIAEEALPAFDALLAA"
     gene            complement(890..1705)
                     /locus_tag="VFF98_15705"
     CDS             complement(890..1705)
                     /locus_tag="VFF98_15705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZU65132.1"
                     /translation="MSGAPMNGLVPLGSAPANQWQVDSARVDLVRSQPAPRPLDLAAS
                     PQRLRLDLARTALVIIDMQNDFCHPEGWLASIGVDVGPARVPIAPLVAVRSACAAAGM
                     PVVWVNWGNRADRLNLGPATLHVYDADGSGGGLGARIGARGKVLEAGTWCAAVVEEFG
                     DTSAEIHVAKYSMSGFWDTPLDAILRNLGVTTLLMGGVNLDQCVLATLQDASFRGYDC
                     VLLEECAATTSPEFCARAALYNIRQCFGFTASAADLLDALAEPACAATRGVSA"
     gene            complement(2020..2178)
                     /locus_tag="VFF98_15710"
     CDS             complement(2020..2178)
                     /locus_tag="VFF98_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65133.1"
                     /translation="MSEPARARAVLSTEDFKLIREAVLFYLKAHEDAPESIKFSNLYH
                     RLGSAAGR"
     gene            complement(2313..3911)
                     /locus_tag="VFF98_15715"
     CDS             complement(2313..3911)
                     /locus_tag="VFF98_15715"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZU65134.1"
                     /translation="MTYQQTIAEADQTIGQSRQTWTGIEAENVARMRLQNRFRTGLDI
                     ARYTARIMRADMAAYDADPANYTQSLGCWHGFIGQQKLISIKKHFGTTKGRYLYLSGW
                     MVAALRSEFGPLPDQSMHEKTSVSALIEELYTFLKQADARELGGMFRELDKARKEGNA
                     VEAARIQHAVDNYETHVVPIVADIDAGFGNAEATYLLAKKFIEAGACCIQIENQVSDE
                     KQCGHQDGKVTVPHEDFLAKIRAVRYAFMELGVDEGVIVARTDSLGAGLTKQIAFVRE
                     AGDIADQYNSFLDVEEVDTAAIGHGDVLITRNGKLVRPKRLPSNLFQFRAGTGEDRCV
                     LDCIASLQNGADLLWIETEKPHIGQIGGMVNRIREVIPNAKLVYNNSPSFNWTLNFRQ
                     QVFDRWQQEGKDVSAYDRARLMSVDYDTTELAIEADERIRTFQRDAAREAGIFHHLIT
                     LPTYHTAALSTDNLAKEYFGEAGMLGYVKGVQRQEIRQGIACVKHQNMAGSDVGDDHK
                     EYFAGEAALKAAGKDNTMNQFAAA"
     gene            4065..5483
                     /locus_tag="VFF98_15720"
     CDS             4065..5483
                     /locus_tag="VFF98_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HZU65135.1"
                     /translation="MTTARTAPRPVYMGPRLKRLRRELGLTQQAMADELAISPSYVAL
                     IERNQRPVTAELLLRIARSYRLDLADLAVDEGADYARRLGQVLRDPIFADIDLPALEV
                     ADLAASFPGVSEALLRLYGAYTREQAALADLHAAPDGGAAAVPDPVAELRRFIAGRSN
                     FFASLDARGEALAAAAAEDGDLQTHLARRHGVRTRLLPAHVMMGALRRFDRHNQQLLL
                     DDTLAPASRAFQIALQLGQSAARADIVALAREAGFASPTAQALARRALADYLAAAILM
                     PYDRFARMADERAYDIEALSRLFGTSFEQTAHRLTTLHKPGAERVPFFFLRVDQAGNV
                     SKRLDGAGFPFAGHGGGCPLWNVHQAFAQPGRILTQWLELPDGKRYFSLARTVRSGGG
                     SLGRPEVERAVALVCAADQAPRLAYAAGAEPARAVATPIGVNCRLCQRPDCAARALPP
                     IGREILSDEYRRTGAPFVFSET"
     gene            complement(5484..6656)
                     /locus_tag="VFF98_15725"
     CDS             complement(5484..6656)
                     /locus_tag="VFF98_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZU65136.1"
                     /translation="MMQGMDLPELGLLLLVACCVAIGSARVRLPYAVGLVAAGLALGL
                     AGFPGRAALTGHLLFGAILPPLIFEAALHLGWGRFRREAPLVLSLAFIGTALSAAVVA
                     GLMHGLAGWGWPAALLFGALIAATDPVSVIAMMKEQQAEPRLRFLMEAESIVNDGAAA
                     VLFALVAGVIEGQAASPSAMALALATTVGGGVLAGLAVGAALMLMAGRASDPLVEIAL
                     TLLAAYGAFWLADGLHVSGVLATLAAGMLVGNWGKGRHLSAAGGQAALAFWEFAAFLA
                     NSLVFLLIGSREAAQPITAYLWPAAMGTLAVLAGRAAAIYPLGALFARSRLAMDRLTQ
                     HILFWGGLRGALAIALALEAPASLPEHAALVSTAFAVVAFSIFVQGLSVPRLLARA"
     gene            complement(6729..8393)
                     /locus_tag="VFF98_15730"
     CDS             complement(6729..8393)
                     /locus_tag="VFF98_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZU65137.1"
                     /translation="MIDRRHFIGGATALATAPAFAATGQGGLDMALVNGDFWTGHGLP
                     NGPRPRALGIIGGRIAALGDAAVRARITRRTQVIDLDGAFALPAFADGHTHFLKGAVA
                     LSQPDLLQARSQAEFTALVAAAVRKAPGQWLLGGAWDEQRMGGQLPTRAWLDAVSPDT
                     PIALPRTDLHSLLLNSVALRLAGITRDTPDPDGGMILRDAQGEPTGVLKDNAKALVLR
                     IIPAPSEAETDAALRAGIAHGLSKGFAQIHNPEIDWTVLPALRRLRAAGETDLRFYCF
                     VPIHDWARLVEIIKADGRGDDWVRWGGVKALADGSLGARTAAFFGDYADAPGQKGLWV
                     TPPDELRELVRAADAHGLHVATHAIGDRANATVLDIYAEVARAHGPRDRRFRIEHAQH
                     MRLADIPRFRAQNVIASVQPFHAIDDGRWAVKRIGEARLAGTYAFRSFFEAGVKMAFG
                     SDWPVAPLDPFVGIAAAVLRETIDGANPHGWQPQQRVSVEQALKAYTQGNAYAAFMDD
                     RAGLLAPGYYADITVLDRDLTAVRPEEILATKVLKTIVNGRLRYEG"
     gene            complement(8390..>9415)
                     /locus_tag="VFF98_15735"
     CDS             complement(8390..>9415)
                     /locus_tag="VFF98_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZU65138.1"
                     /translation="HRPPAPPSSGCPMDTSLIDPEIRQFIARMRADWDRHPPLASLPL
                     PQARAVAEEVRAPWRQGGPAMALTRELNADTAAGPLRLRLHYPAAPAPTDPAPTAPNA
                     GALLPVLAYLHGGGFTLFSIDTHDRLMREYAAAAGCVVVGVDYPLAPEAKYPVALNRL
                     VALAGWLAEHGAALGLDAARLALGGDSAGGNLALAAALRLRDAGRGHAICGLLLNYGA
                     FDSHCSDAAEALHGGPGAILDRAEMQYYWANYLRTPADAEDPYACPIHAALAGLPPCF
                     LAVAELDVIAEQSHAMAAALAAAGVRADLRVYQGATHSFLEAMSVSALARAAIADGAA
                     FLKEVLA"
BASE COUNT         1450 a         3237 c         3257 g         1471 t
ORIGIN      
        1 tgggcggcca gcagcgcgtc gaaggcgggc agcgcttcct cggcgatcat cgtcagcacc
       61 agcgggcagg cgccggtgtc aagatcggcc accgggcgga tcaggctggc ggtggtggcg
      121 ccatagatcg tcagctgccg cttggggatg gccccatcgg cgcccagcgc ggcggcaaag
      181 gccagcgttg tcggcaagtc gtcgaaagcg acgatgcggt cgtgccaggc gcggcgcggg
      241 gccagcgcca gctccacttc caggatcacg ccattggtgc cataggcatg caccacggcc
      301 tgggcatcgc gcccggtcag ctcgaggatg cgcgggcttt cctcggcggt gagcaccttg
      361 gccgcgcgca cattgtcgcc gctgtgcagc gtgccaaagc cgatggcgcc gcagccggtg
      421 gagccgcccg ccacaaagcc gccgatcgtc gcgcttttcc aggtggaggg gaagaagcgc
      481 agctcgtagc cggcggcatt caccgcctgc tcgatgtcat acatcttggc gcccgcctcg
      541 gcgcgcacca cgccatcgcc gatgtggcag atggcggtca gcctggtcat gtccatcacc
      601 acgccgccct tcagtggcat ggcctggcca taattgcccg tgccgccgcc gcgcagggtg
      661 agcggcaggc ggttttccac gcaaaagcgc accacctggc tcacttcccc ggcgctggcg
      721 ggggtaacga tggcatcggc gcgcacctct tccagctgcg ccttcagcac cggtgagaac
      781 cagtagtaat cgcgcgactt gcgcttgatc accttttcgg cggtgctcac ctcgatgggg
      841 ctgaggcgtt ggctcagcgc gtcccagtcg atcagggtgg cggcggtggt catgcggaaa
      901 ctccccgggt ggcggcgcag gccggctctg ccagcgcgtc gagcaggtcg gcggcgctgg
      961 cggtgaagcc gaaacactgg cggatgttgt aaagcgcggc gcgcgcgcag aattcgggcg
     1021 aagtggtggc cgcgcattct tccagcagca cgcaatcata gccgcggaag ctggcatcct
     1081 gcagcgtggc cagcacgcat tgatcgaggt tgaccccgcc catcagcagg gtggtgacgc
     1141 ccagattgcg caggatcgca tcgagcggcg tatcccagaa gcccgacatc gaatatttgg
     1201 cgacatggat ctcggcggaa gtgtcgccaa attcctcgac cacggccgcg caccaggtgc
     1261 ccgcttccag caccttgccg cgcgcgccga tgcgggcgcc aaggccgccg ccgctgccat
     1321 cggcatcata gacatgaagc gtggccgggc ccaggttcag ccggtctgcc cggttgcccc
     1381 agttcaccca caccaccggc atgccggccg ccgcgcaagc cgatcgcacc gctaccagcg
     1441 gggcgatcgg cacgcgcgcc gggcccacat cgacgccgat cgaggccagc cagccttccg
     1501 ggtggcagaa atcattctgc atgtcgatga tgacgagcgc cgtgcgcgcg agatcgagcc
     1561 gcaggcgctg cgggctggcg gccagatcga gcgggcgcgg cgccggctgg ctgcgcacca
     1621 gatccaccct ggcgctatcc acctgccact ggttggccgg cgcgctgccc agcggcacca
     1681 agccgttcat gggggccccg ctcatgcgcg ggcagccttg tttgggcccc gcgcccgttg
     1741 aaattcggat cggacggttt ccatccttgc gcacaccctc ctgttgggca agccatgcct
     1801 gttgggcaac caatgttgca gcgcagcaag atggattgca atgcagcaag gctagcaaaa
     1861 tcagggtgct tcaaaagcgt ttaattgcgc acgaagtgtt gccttttggg catcatggga
     1921 aaggcgtggt ccgcccgctc cacgaaaaga ggcccgcagc gccttggggg cgttgcgggc
     1981 cgtgtctcct ccccaggaga aagtgtgtgt ggctgtggct cagcgcccgg cggcgctgcc
     2041 cagccggtga tagagattgg aaaacttgat cgattccggc gcgtcttcat gcgccttcag
     2101 atagaacagt acggcctcgc ggatcagctt gaagtcctcc gtggagagga cggcgcgggc
     2161 acgggcaggt tcgctcatgg tcttgctcct cgttgtgacg cctgaagtgg cgccggactt
     2221 tccgctcgtg gggtggccgg gggctgcggg ttatgtctgt cccgcatgcc cccggggtgc
     2281 gaccctcgcc gcgaaggggg tgttcccggc cgtcaggcgg ccgcgaactg gttcatcgtg
     2341 ttgtccttgc cggcggcctt cagggccgct tcgccggcga aatattcctt gtgatcatcg
     2401 cccacgtcgc tgccggccat gttctggtgc ttcacgcagg cgatgccctg gcggatctcc
     2461 tggcgctgca cgcccttgac gtaacccagc atgcccgcct cgccgaaata ttccttggcc
     2521 aggttgtcgg tgctgagcgc tgcggtgtga taggtgggca gggtgatcag gtggtggaaa
     2581 atgcccgcct cgcgcgccgc atcgcgctgg aaggtgcgga tgcgctcgtc ggcctcgatg
     2641 gccagctcgg tggtgtcata gtccacgctc atcagccgcg cccggtcata ggccgacaca
     2701 tccttgccct cctgctgcca gcgatcgaac acctgctggc ggaagttcag cgtccagttg
     2761 aagctgggcg agttgttgta gaccagcttg gcattgggga tcacctcgcg gatgcggttc
     2821 accatgccgc cgatctggcc gatatggggc ttctcggtct cgatccacag aaggtcggcg
     2881 ccgttctgca ggctggcgat gcaatccagc acgcagcggt cctcaccggt gccagcgcgg
     2941 aactggaaca ggttggaagg caggcgcttg gggcgcacca gcttgccgtt gcgagtgatc
     3001 agcacatcgc catggccgat cgcggcggtg tccacctctt ccacatcgag gaagctgttg
     3061 tactggtcgg cgatgtcgcc agcttcacgc acaaaggcga tctgcttggt aaggccggcg
     3121 cccagcgagt cggtgcgggc cacgatcacg ccctcgtcca cgcccagctc catgaaggca
     3181 tagcgcaccg cgcggatctt cgcgaggaag tcctcgtggg gcacggtcac cttgccgtcc
     3241 tggtggccgc actgcttctc gtccgagacc tggttctcga tctggatgca gcaggcgccc
     3301 gcctcgatga acttcttggc gaggaggtag gttgcctcgg cattgccgaa accggcatcg
     3361 atatcggcca cgatgggaac cacatgggtt tcataattgt cgaccgcgtg ctggatgcgg
     3421 gcggcttcca ccgcgttgcc ttccttgcgg gccttgtcca gctcgcggaa catgccgccc
     3481 agctcgcggg catcggcctg cttgaggaag gtgtacagct cctcgatcag cgccgagacc
     3541 gaggtcttct cgtgcatcga ctggtcgggc agggggccga attcgctgcg cagggcagcc
     3601 accatccagc ccgacaggta gaggtagcgg cccttggtgg tgccgaagtg cttcttgatc
     3661 gagatcagct tctgctgccc gatgaagccg tgccagcagc ccagcgactg ggtgtagttg
     3721 gccggatccg cgtcataggc ggccatgtcg gcgcgcatga tgcgggcggt gtagcgggcg
     3781 atgtcgagcc cggtgcggaa acggttctgc aggcgcatgc gggccacgtt ctcggcctcg
     3841 atgccggtcc aggtctggcg gctctggccg atcgtctgat ccgcttcggc gatggtctgc
     3901 tggtaggtca tggggaactc cgctccagtt cgtctgtgcc cgctgccggg cgtctgaagc
     3961 gtgtgtatgc tgccccgaag ggcgcgggaa ggcgcggcga agtgccgttg taaggcttta
     4021 cagattatcc ttgtaaagtt gtgcagacat gacaggatgc gcccatgacc accgcccgca
     4081 ccgctccccg cccggtctat atgggccccc gcctcaagcg cctgcgccgc gagctgggcc
     4141 tcacccagca ggcgatggcc gacgagctgg cgatctcgcc ctcctatgtc gcgctgatcg
     4201 agcgcaacca gcgccccgtc acggccgagc tgttgctgcg catcgcccgc agctaccggc
     4261 tcgatctggc cgatctggcg gtggacgagg gcgccgatta tgcccgccgg ctgggccagg
     4321 tgctgcgcga tccgatcttt gccgatatcg atctgccggc gctggaagtg gcggatctgg
     4381 cggcgtcctt ccccggcgtt tcggaagcgc tgctgcggct ctatggcgcc tacacgcgtg
     4441 agcaggcggc gctggcggat ctgcatgccg cgcccgatgg cggcgctgcc gccgtgcctg
     4501 atccggtggc cgaactgcgc cgcttcatcg ccggccgctc caacttcttc gccagccttg
     4561 atgcgcgcgg cgaggcgctg gccgccgctg ctgccgagga tggcgatctg caaacccatt
     4621 tggcacgccg gcatggcgtg cgcacgcgcc tgctgcccgc ccatgtgatg atgggcgccc
     4681 tgcgccggtt cgaccggcac aaccagcaat tgctgctgga cgacacgttg gcgcccgcct
     4741 cccgcgcgtt ccagatcgcc ctgcaactgg gccagagcgc ggcgcgcgcc gatatcgtgg
     4801 cgctggcgcg cgaggccggc tttgccagcc ccacggcgca ggccctggcc cgccgcgcgc
     4861 tggccgatta cctcgccgcc gcgatcctca tgccctacga ccgctttgcc cgcatggccg
     4921 acgagcgcgc ctacgacatc gaggcgctct cccgcctgtt cggcaccagc ttcgagcaga
     4981 ccgcccaccg cctcaccacc ctccacaagc cgggggcaga acgcgtgccc ttcttcttcc
     5041 tgcgcgtgga tcaggcgggc aatgtctcca agcggctgga tggcgcgggc tttccctttg
     5101 ccggccatgg cggcggctgc ccgctgtgga atgtgcacca ggcctttgct cagcccggcc
     5161 gcatccttac gcagtggctg gaactgcccg atggcaagcg ctatttctcg ctggcgcgca
     5221 cggtgcgctc gggcgggggc agcctgggcc ggccggaagt ggaacgggcg gtggcgctgg
     5281 tctgcgcggc cgatcaggcg ccgcgcctgg cctatgccgc aggggcagag cccgcccggg
     5341 ccgtcgccac gccgatcggc gtcaactgcc gcctgtgcca gcggcccgat tgcgccgccc
     5401 gcgccctgcc gccgatcggc cgcgaaatcc tttccgacga atatcgccgc acgggcgcgc
     5461 ccttcgtgtt ttcggagacg tgatcaggcc cgcgccagca accgcggcac gctcaacccc
     5521 tgcacgaaga tcgagaaggc gaccaccgcg aaagccgtgc tgaccagcgc tgcatgttcc
     5581 ggcaggctgg cgggggcctc cagcgccagg gcaatggcca gcgcgccgcg caggccgccc
     5641 cagaacagga tgtgctgcgt cagccggtcc atcgccagcc ggctgcgggc aaacagcgcg
     5701 ccgaggggat agatcgccgc cgcgcggccg gccagcaccg ccagcgtgcc catggccgcc
     5761 ggccacagat aggcggtgat gggctgggcc gcctcgcggc tgccgatcag caggaagacg
     5821 agggaattgg caaggaaggc ggcaaattcc cagaaggcca gcgccgcctg cccgcccgct
     5881 gcgctgaggt gccgcccctt gccccaattg cccaccagca tgcccgccgc cagcgtggcc
     5941 agcacgcccg agacatggag cccatcggcc agccagaaag cgccataggc cgccagcagg
     6001 gtgagggcga tctccaccag cggatcgctg gcgcgcccgg ccatcagcat cagcgccgcg
     6061 cccaccgcca gccccgccag cacgccgccg cccactgttg ttgccagcgc cagcgccatg
     6121 gccgaagggc tggccgcctg cccctcgatc acgccggcca ccagcgcgaa aagcaccgcc
     6181 gccgcgccat cgttgacgat gctttccgct tccatcagga agcgcaggcg cggctctgcc
     6241 tgctgctcct tcatcatggc gatcaccgag acaggatcgg tggcggcgat cagcgcgccg
     6301 aacagcagcg ctgccggcca gccccagccc gccagcccat gcatcagccc ggccactacc
     6361 gcggccgaca gcgccgtgcc gataaacgcc agcgagagca ccagcggcgc ctcgcggcga
     6421 aaacggcccc agcccagatg cagcgccgcc tcgaagatca gcggcggcag gatggcgccg
     6481 aacagcaagt ggccggtgag cgccgcccgg ccgggaaagc ccgccagccc cagcgccagc
     6541 ccggccgcca ccagccccac cgcatagggc agccgcaccc tggccgagcc aatcgccacg
     6601 cagcaggcca ccagcagcag caggcccagt tccggcagat ccattccctg catcatgctt
     6661 tgcgtcatgc gggtaagggt agggccgggc gggccggttg caaagcccat cctcggccca
     6721 gccccggttc aaccctcgta gcgcaggcgc ccgttgacga ttgtcttcag caccttggtg
     6781 gcgaggattt cttccgggcg cacggcggtc aggtcgcggt cgagcacggt gatgtcggcg
     6841 taatagcccg gggccagcag gccggcgcgg tcgtccatga aggcggcata ggcgttgcct
     6901 tgcgtatagg ccttaagcgc ctgttccacg ctgacgcgct gctggggctg ccagccatgg
     6961 gggttggcgc catcgatcgt ctcgcgcagc acggccgccg caatgccgac gaagggatcg
     7021 agcggggcca ccggccagtc cgatccgaag gccatcttca cgcccgcctc aaagaaggag
     7081 cggaaggcat aggtgccggc cagccttgcc tcgccgatgc gtttcaccgc ccagcgcccg
     7141 tcgtcgatgg cgtggaaggg ctgcaccgag gcgatgacgt tctgcgcgcg aaagcgggga
     7201 atgtcggcca ggcgcatgtg ctgggcatgt tcgatgcgaa agcgccgatc acgcgggcca
     7261 tgggcgcggg ccacttcggc gtagatgtcg agcacggtgg cattggccct gtcgccgatg
     7321 gcatgggtgg ccacatgcag gccgtgtgcg tcggctgcgc gcaccagttc gcgcaattcg
     7381 tcgggcgggg tcacccacag gcccttctgg ccgggcgcat cggcataatc gccgaagaag
     7441 gcggccgtgc gcgcgcccag cgagccatcg gccagcgcct tcacgccccc ccagcgcacc
     7501 cagtcatcgc cccggccatc ggccttgatg atctccacca gccgggccca atcgtggatc
     7561 ggcacgaagc agtagaagcg caggtccgtc tcgccggccg cgcgcaggcg gcgcagggcc
     7621 ggcagcaccg tccagtcgat ctcgggattg tggatctggg caaagccttt ggacaggcca
     7681 tgggcaatgc cggcgcgcag ggccgcatcc gtctcggcct cggaaggggc ggggatgatg
     7741 cgcagcacca gcgccttggc attgtccttc agcacgccgg tcggctcgcc ctgggcatcg
     7801 cgcaggatca tgccgccatc gggatcgggc gtgtcacggg tgatgccggc caggcgcagc
     7861 gccaccgaat tgagcagcag cgaatgcaga tccgtgcgcg gcagggcgat tggtgtgtcg
     7921 ggcgagacgg catcgagcca ggcgcgggtg ggcagctggc cgcccatgcg ctgctcgtcc
     7981 caggcgccgc ccagcagcca ttggccgggc gccttgcgca cggcggcggc caccagcgcg
     8041 gtgaattcgg cctggctgcg cgcctgcagc aggtctggct ggctgagcgc caccgcgcct
     8101 ttcaggaaat gggtgtggcc atcggcaaag gcgggcaggg caaaggcgcc gtcaaggtcg
     8161 atcacctgcg tgcggcgggt gatgcgcgcg cgcaccgccg catcgcccag cgccgcgatc
     8221 cgcccgccga tgatgcccag cgcgcgcggg cgtggcccgt tcggcaggcc atgcccggtc
     8281 cagaaatcgc cgttcaccag cgccatgtcg aggccgccct ggccggttgc ggcaaaagcg
     8341 ggcgctgtgg ccagcgctgt ggccccgccg atgaagtgcc gcctgtcgat catgccagaa
     8401 cctccttcag gaaagcggcg ccatcggcaa tggccgcgcg cgccagcgcc gagacggaca
     8461 tcgcctcgag gaaactgtgg gtggcgccct gatagacgcg cagatcggcc cgcacgccgg
     8521 cggcggccag ggccgctgcc atggcatggc tctgctcggc gatcacgtcg agttcggcca
     8581 cggccaggaa gcagggcggc aggcccgcca gcgcggcatg gatggggcag gcgtaggggt
     8641 cttcggcatc ggccggcgtg cggaggtaat tggcccagta atactgcatc tcggcccggt
     8701 cgaggatggc gccggggccg ccatggagcg cttcggccgc atccgagcaa tggctgtcga
     8761 aggcgccata gttaagcagc aggccgcaga tggcatggcc gcgcccggca tcgcgcaggc
     8821 gaagggccgc tgccagcgcc agattgccgc cggccgaatc gccccccagc gccagccttg
     8881 ccgcatccag ccccagcgcc gcgccatgct ctgccagcca gcccgccagt gccaccagcc
     8941 ggttgagcgc gaccggatat ttcgcttcgg gcgccagcgg ataatccacc cccaccacca
     9001 cgcagccggc cgccgctgca tattcgcgca tcagccggtc atgcgtgtcg atggaaaaca
     9061 gcgtgaagcc gccgccatgc agataggcca gcaccggcag cagcgcgcct gcgtttggcg
     9121 ctgtgggggc ggggtctgtg ggggcggggg cggcgggata atgcaggcgc aggcgcagcg
     9181 ggccggcggc ggtgtcggca ttcagctcgc gggtgagggc catggcgggg ccgccctgcc
     9241 gccagggcgc gcgcacctcc tcggccacgg cgcgcgcctg gggcagcggc aggctggcca
     9301 gcggcgggtg gcggtcccag tcggcgcgca tgcgggcgat gaactggcgg atttcgggat
     9361 cgatcagcga ggtgtccatg gggcatccgg aagaaggcgg ggcagggggc cggtg
//
LOCUS       DASRYE010000308        13782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_187755,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000308 DASRYE010000000
VERSION     DASRYE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187755"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFF98_15740"
     CDS             complement(<1..1283)
                     /locus_tag="VFF98_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HZU65139.1"
                     /translation="MKLSLKQKLAAAFSAVLLLTAILGGVGIASMGKINAQANEINTN
                     WMPSIDAIHNIEAGVAELRALELTHIANTDPAAMADVEKRIHATLDKIAEQRSRYARL
                     VSSPEEQAGYDRFSRAFDRYLTIHEEILKHSRINENAKATAILFSTADIYNDMTATLR
                     TLVKINVDGGNAAALSGDATYANARALFIGIIVAALGIGTGAALVVVRGVMRQLGGEP
                     DYARSIISQIAEGDLTLRIATREGDTTSLLAAARDMVARLQQVIGDALVAAGNVASGA
                     QQMAAGSETLSQGATEQAAATEEASSSVEEMAGNIKQNADNAAQTEKIARQSAKDADM
                     SGTAVRKAVDAMQVIATKISVVQEIARQTDLLALNAAVEAARAGEHGRGFAVVASEVR
                     KLAERSQVAAQEIGEVAGSSVELAEKAGKLLDEIVP"
     gene            complement(1308..1820)
                     /locus_tag="VFF98_15745"
     CDS             complement(1308..1820)
                     /locus_tag="VFF98_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZU65140.1"
                     /translation="MNAPASHSLPAIAIPERMLTFGLGGEHFAIDAQCVREIMEVPAI
                     TRVPGAPAFADGLLNVRGAIVPLADLAIVFGMERAAHNADTRIIVIEAAVEEEAATIG
                     ILADKVFDVASLDGAPVESPPQVGMKWRTDFIIGIARHAGRFVILPDLPRIFTECVGQ
                     IHRAAPHLPA"
     gene            complement(1817..3832)
                     /locus_tag="VFF98_15750"
     CDS             complement(1817..3832)
                     /locus_tag="VFF98_15750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZU65141.1"
                     /translation="MSDGADPVAAFRIEAGELLDQVERALLDLTGAPAHRGLIDEVFR
                     GLHTLKGSGAMFGFDALARFTHHCESAFDRVRKGQAPASRALIDVVLSARDHMQALID
                     AGNSSDPALDGAGEAILARLASAVATAPAAAAMDAAPAPEAAPPEAEAGWQIRFRLPE
                     GALANGSNPLALLDELRDLGPCTVVCRMGELPALDAFAPAECHAAWEVTWAAADAGEI
                     SREAIEDVFLFLIDDMELTITRPGAPAATADAPPLPADTAEGPAAPRPAGPPRESVRV
                     QAERLDELMNRVGELVIAQSRLELLAMAQGQVDAASLRAVSEDIQRLSAELRDTSMAL
                     RMMPVAHLFGRFRRLIHDLAQETGKSIELVTDGEATEVDKTVIERLADPMIHVIRNSC
                     DHGLEPAGERAAAGKPATGRIVLSARQAGGEVLIAIRDDGRGINRARVRAKAEAQGLI
                     APGAPLADAELLQMIFAPGFSTAAAITNLSGRGVGMDVVKRSIETLRGTIDIDSTEGD
                     GTTITLHLPLTLAIIECMLVRVGAGRYAIPLSAVEECLELPATEVAGTRRRNFLDVRG
                     ELVPYLRLHDSFASDHPADLYQKVIVVAQGRGRVGLVVDQILGNTQTVIKSLSRFHAG
                     VSAFSGATILGDGSVALILDVGALIAGAQKARRTRGDRPAESAGEAA"
     gene            complement(3829..4197)
                     /locus_tag="VFF98_15755"
     CDS             complement(3829..4197)
                     /locus_tag="VFF98_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZU65142.1"
                     /translation="MTASILTVDDSPSLRMAVRIALTGAGYNVVEAGDGMEGLSKAGT
                     QRFDLIITDLNMPRMDGLTMIRELRKSPTQTGVPIVFLTTESEDGLKAEAKAAGATGW
                     LVKPFNADQLVKVARKVLGR"
     gene            complement(4194..4499)
                     /locus_tag="VFF98_15760"
     CDS             complement(4194..4499)
                     /locus_tag="VFF98_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZU65143.1"
                     /translation="MPTFTLPAQATIQNICALHTELLAALDGAADDICVATADLVQAD
                     FSLLQLLVAAQAEACRRGRRLLLESPAHPLLADLLARAGLNAAAAQDVASWLPGECA"
     gene            complement(4648..4941)
                     /locus_tag="VFF98_15765"
     CDS             complement(4648..4941)
                     /locus_tag="VFF98_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65144.1"
                     /translation="MAASMGPALPMAHWLAAAEQGEADACFSLGVAFSTGDHGAPCDL
                     VEAHKWFNLAAAHGHEEAPAARAEVAEDMSAREIAEAQRRARAWLAQQRRHAA"
     gene            5260..5718
                     /gene="dksA"
                     /locus_tag="VFF98_15770"
     CDS             5260..5718
                     /gene="dksA"
                     /locus_tag="VFF98_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZU65145.1"
                     /translation="MANGLADDIDVLVKARRSLAGDYVPTDDETYMSEPQLDYFRRLL
                     QQWKKLILSAAADTLSQLQEGPLREPDLNDRASSETDWGIELRTRDRQRKLIAKIDSA
                     LRRIEEGEYGFCEVTGEPIGLGRLVARPIATMTVEAQEAHERREKISRDD"
     gene            5858..6241
                     /locus_tag="VFF98_15775"
     CDS             5858..6241
                     /locus_tag="VFF98_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZU65146.1"
                     /translation="MSDSDQRLQPRDSLFLLARLRLVGDVAEWRVKVRNLSASGLMAE
                     GDIDIQRGTAVQIELRNLGWVNGLVAWVQGNRLGVALEQEIDPKSVRAQPDATAADER
                     AYYHRPSLTAAAQTHAPDPRRLRKI"
     gene            6314..7870
                     /locus_tag="VFF98_15780"
     CDS             6314..7870
                     /locus_tag="VFF98_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU65147.1"
                     /translation="MLAGDTLSLLPLERLPPLPPKRQGLWRAPALLGICALLAACGHG
                     QGQTVDVAVIGAPASLFAAPASPPAAASADQHLLQHLLLSATREGLVAFDEEGKIVPA
                     IADRWIVADDGQSFIFRLRDGTWPDGAGLTGDSVRQALHAAIEAARKGPLAGDLAMIT
                     DVRAMAGRVVEIRLTQPMPDFLQLLAQPELGVMHHGKGTGPMLLHREGDAAVLVPIAP
                     EKRGAPAVEGWADMAHDLRLRALPADAALAELAAGHVQVVLGGGFADLPRLRARALDQ
                     RQLRLDPVSGLFGLAVADTGGLLATPQLREAIAMAIDRDSLATTIAPAGWAASSRVLP
                     TGLGSESLATAERWSDIDLDGRRTLALRRIALWAGAAHQHPVLRIALPEGPGADLLFD
                     RLSNDLADVEVGAQRVGMDQPADLRLVDEVAPYARLSWFFSRLSCRVRGPGCAPQADA
                     LAQQAALAADPASARALYGEAGRQLAEANVFIPFGPPIRWSAVAGGTTGFAINRIAVH
                     PLLPLAIRPR"
     gene            8025..8720
                     /locus_tag="VFF98_15785"
     CDS             8025..8720
                     /locus_tag="VFF98_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZU65148.1"
                     /translation="MRVLLIEDEPATARAIELMLTTEGFNVYSTDLGEEGLDLGKLYD
                     YDIILLDLNLPDMHGYDVLKKLRVAKVQTPVLILSGISEMDSKVRSFGFGADDYVTKP
                     FHREELIARIHAVVRRSKGHSQSVIRTGKLCVNLDAKTVEVDSARVHLTGKEYAMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAHACGGENYIETVWGRG
                     YVLRDPDEAAFAA"
     gene            complement(8828..9544)
                     /locus_tag="VFF98_15790"
     CDS             complement(8828..9544)
                     /locus_tag="VFF98_15790"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HZU65149.1"
                     /translation="MTAHKDGDPTTLNRLYGRAKGKPLRAGQQALVDDLLPRIAVPAE
                     GPVTAEGLFGEACPLHFEIGFGGGEHMAFRADMLPGHGFIGAEPFLNGVAQALTHVAG
                     DNGQHPPLGNVRIHHGDALEVLRRIPDGALSFLYLLHPDPWPKARHAKRRMMNDGPVD
                     LFAAKLKPGGELRFGTDHAIYLRHALMVMQRHTHQFEWLASTASDFQNRPSGWPETRY
                     EAKARNVYGHEVWYFRYRRK"
     gene            9668..9838
                     /locus_tag="VFF98_15795"
     CDS             9668..9838
                     /locus_tag="VFF98_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65150.1"
                     /translation="MIVFGCLGLIVLPLLGFFAGLWLGGPLVGAWCAGAGFVLAAALS
                     VVMTYAMVKARA"
     gene            complement(9896..12160)
                     /locus_tag="VFF98_15800"
     CDS             complement(9896..12160)
                     /locus_tag="VFF98_15800"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZU65151.1"
                     /translation="MSEESNVRFTEREALFYHNTIRPGKIEIIASKPMATQRDLSLAY
                     SPGVAVPVQAIADNPATAYDYTARGNLVAVISNGTAILGMGNLGALASKPVMEGKAVL
                     FKRFADVDSIDIEMASEDPEALIEAIALMEPSFGGINLEDIKAPECFVIEQALRERMK
                     IPVMHDDQHGTAIIAAAGLINACLLTNRELKDIKVVVNGAGASALACTALIKSMGVPH
                     QNVVVCDRQGTIWRGRETGMDQWKSAHAVDTPARTLEEALVGADVFIGLSAAGALKAD
                     YVKKMAAEPIIFAMANPDPEITPPEARAARPDAIIATGRSDYPNQVNNVLGFPFIFRG
                     ALDVRATAINEEMKIAAANAIAELARQQVPEEVALAYGRNHSFGRDYIIPAPFDPRLM
                     EIVPSAVAKAAMETGVAQKPIEDFDAYRHQLKARLNPTTSVLTQVYAQVKENPKRVIF
                     AEAENEVVLRAAIQFRDFGYGTPVLVGRTQAVRDKLVELGVGNPDGWEIHNAADSVHV
                     EAMGRMLYDRLQRRGYLERDIKRMVNQDRNAFASALLKLGVGDALVTGITRPFAQTMK
                     EVRHVLDPKPGHLPFGIHVMVGKNTTVFLADTTCNERPTAEDLAVIAKETAAVARRLG
                     HEPRVAFLSYSTFGNPQGRWLEPLRGAIAILDEEQPGFEYEGEVAPDAALNPTVMKLY
                     PFSRLTAPANVLIFPGLQSANIAAKLLRELGGNTAIGPMLVGMEKPVQIVPMTAIAPD
                     VLTLAVLAAAEVAG"
     gene            12274..>13782
                     /locus_tag="VFF98_15805"
     CDS             12274..>13782
                     /locus_tag="VFF98_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZU65152.1"
                     /translation="MSDAPTPMMSQYLALKAEAGNSLLFYRMGDFFELFFEDARLAAQ
                     ILDIALTSRGEHAGAPIPMCGVPVHAAENYLARLIRAGCRVAIAEQVETPEEAKKRGG
                     SKALVRRAIVRFVTAGTLTEEALLEPRRANVLAALCELRGAIGIASVDISTGRMELEE
                     CAPEQLGAALARIGASELVASDGWDAGPLEATPRPAADFASEAGTQRLCALHGVGTLD
                     GFGSFSRAMLAAAAGLVAYLDHVGRGSLPLLLPPVARVHAGHLAMDEATRASLEILQS
                     AAGAREGSLVATVDRCVTGPGARQLAEDLAAPLMDRAAIGARLALVGWLHEEALLRED
                     LRAALRGLPDVGRALGRVVAGRGSPRDLGQLRDGLAGAARLAALLAAQTARPALAEAL
                     LPTLEGHEPLIELFTRALVATPPTERGQGGFIAAGYDAALDELRAISRDARVAIAALE
                     AKYREETGIAALKIKHNGVLGYFIEVPAARADALLAAGSGFTHRQTMAGAMRF"
BASE COUNT         2103 a         4525 c         4798 g         2356 t
ORIGIN      
        1 ggcacgattt catccaacag cttgccggct ttttccgcca attcgacgct ggaaccggcc
       61 acttcgccga tttcttgcgc ggccacctgg ctgcgctcgg ccagcttgcg cacttcgctg
      121 gccaccacgg caaagccgcg gccatgttcg ccggcgcggg ccgcttccac ggccgcgttc
      181 agcgcaagga ggtcggtctg gcgggcaatc tcctgcacca ccgagatctt ggtggcgatc
      241 acctgcattg catccaccgc cttgcgcacg gcggtgccgc tcatatcggc atccttggcc
      301 gactggcggg cgatcttctc ggtctgggcg gcattgtcgg cgttctgctt gatgttgcct
      361 gccatctctt ccaccgagct ggaggcctcc tcggtggccg cggcctgttc ggtggcgccc
      421 tgcgagaggg tttccgagcc cgccgccatc tgctgcgcgc ccgaggcgac attgccggcc
      481 gccaccagcg catcgccgat cacctgctgc aggcgggcca ccatgtcgcg cgcggcggcc
      541 agcaggctgg tggtgtcgcc ctcgcgggtg gcaatgcgca aggtgaggtc gccctcggcg
      601 atctggctga tgatcgagcg ggcatagtcc ggctcgccgc ccagctggcg catcacgccg
      661 cgcaccacca ccagcgccgc gcccgtgcca atgcccagcg ccgccacgat gatgccgatg
      721 aacagcgcgc gggcgttggc ataggtggca tcgccgctga gcgcggccgc attgccgcca
      781 tcgacgttga tcttcaccag cgtgcgcaag gttgctgtca tgtcgttgta gatgtcggcg
      841 gtggagaaca ggatcgcggt cgccttggcg ttctcgttga tgcgcgaatg cttgaggatc
      901 tcctcgtgga tcgtcagata gcgatcgaac gcgcgcgaga agcggtcgta gccggcctgt
      961 tcctcgggcg aggaaaccag cctggcatag cggctgcgct gctcggcgat cttgtcgagc
     1021 gtggcgtgga tgcgcttctc gacatcggcc atcgcggccg gatcggtgtt ggcgatatgg
     1081 gtcagctcca gcgcgcgcag ctcggccacg cccgcctcga tgttgtggat ggcatcgatg
     1141 ctgggcatcc agttggtgtt gatctcgttg gcctgcgcgt tgatcttgcc catgctggca
     1201 atgcccaccc cgcccagaat ggcagtgagc aaaagcacgg cggaaaaggc ggccgcaagt
     1261 ttctgtttca ggctcagttt catgatcggt cctcggtgcg ggcggggtca ggcgggaagg
     1321 tggggggcgg cgcggtggat ctggcccacg cattcggtga aaatgcgggg caggtcgggc
     1381 aggatgacaa agcggccggc atggcgggca atgccgatga tgaaatcggt gcgccatttc
     1441 atgcccactt gcggcgggct ttccaccggc gcgccatcaa ggctggccac atcgaacacc
     1501 ttgtcggcca ggatgccgat ggtggcggcc tcttcctcca ccgcggcctc gatgacgatg
     1561 atgcgggtgt cggcattgtg cgcggcccgt tccatgccga aaacgatggc cagatcggcc
     1621 agcggcacga tcgccccgcg cacgttgagc agcccgtcgg caaaggcggg cgcgcccggc
     1681 acgcgggtga tcgccggcac ttccatgatc tcgcgcacgc attgggcatc gatggcaaaa
     1741 tgttcgccgc caaggccgaa ggtgagcatc cgctcgggga tggcgatggc cggcaggctg
     1801 tggctggcag gcgcgttcat gccgcttctc ccgcgctttc agcggggcga tcgccgcgcg
     1861 tgcgccgggc cttctgggcg ccggcgatca gcgcgcccac atcgaggatc agcgccacgc
     1921 tgccatcgcc caggatcgtt gcgcccgaaa aggcgctgac gccggcatga aaacgcgaca
     1981 gcgacttgat gaccgtctgc gtgttgccga ggatctggtc gaccaccagc cccacccgcc
     2041 cgcgcccctg cgcgacgacg atgaccttct ggtagagatc ggccgggtga tcgctggcaa
     2101 aactgtcgtg caggcgcaga tagggcacca gctcgccgcg cacatcgagg aaattgcgcc
     2161 gccgggtgcc ggccacttcg gtggcgggca gctccaggca ttcctccacc gccgacagcg
     2221 ggatcgcata gcgcccggcc cccacccgca ccagcatgca ctcgatgatg gccagggtca
     2281 gcggcaggtg cagggtgatg gtggtgccgt cgccctctgt gctgtcgatg tcgatggtgc
     2341 cgcgcaaggt ttcgatgctg cgcttcacca cgtccatgcc caccccgcgg cccgacaggt
     2401 tggtgatcgc cgccgcggtg gaaaagccgg gggcaaagat catctgcagc aattcggcat
     2461 cggccagcgg cgcgccgggg gcgatcaagc cttgcgcctc ggccttggcg cgcacgcggg
     2521 cgcggttgat gccccggcca tcgtcgcgga tggcgatcag cacctcgccg ccggcctggc
     2581 gcgcgctcag cacgatgcgg ccggtggcgg gcttgccggc ggcggcgcgt tctccggctg
     2641 gctccagccc atgatcgcag ctgttgcgga tgacatggat catcggatcg gccaggcgct
     2701 cgatcacggt cttgtccacc tcggtcgcct cgccgtcggt caccagctcg atgctcttgc
     2761 cggtttcctg ggccagatcg tggatcagcc ggcgaaagcg gccgaacaga tgggccaccg
     2821 gcatcatcct cagcgccatc gaggtatcgc gcagttcggc cgacaggcgc tggatatctt
     2881 cagacaccgc ccgcaggctg gcggcatcca cctggccctg ggccatggcc agcaattcca
     2941 gccggctttg cgcgatgacc agttcgccca cccggttcat caattcatcc agccgctcgg
     3001 cctgcacccg cactgattcg cgcggggggc cggcggggcg gggcgcggct ggcccctcgg
     3061 cggtgtcggc gggcaggggc ggggcatcgg cggtggcggc tggcgcgccg ggccgcgtga
     3121 tggtcagctc catgtcgtcg atgaggaaga gaaacacatc ctcgatcgcc tcgcggctga
     3181 tctcgccggc atctgccgcc gcccaggtca cttcccatgc ggcatggcat tcggccggcg
     3241 caaaggcatc gagcgcgggc agctcgccca tccggcagac caccgtgcag ggccccagat
     3301 cgcgcaattc atccagcagg gccagcgggt tgctgccatt ggccagcgcc ccttcaggca
     3361 ggcgaaagcg gatctgccag cccgcttcgg cctctggcgg ggcggcttcg ggggccgggg
     3421 ctgcatccat cgcggctgca gccggggctg tggccacagc gctggccagc cgggccagaa
     3481 tcgcctcgcc cgccccatcc agcgccggat cgctgctgtt gccggcatcg atcagcgcct
     3541 gcatgtgatc gcgggccgac agcaccacgt cgatcaacgc gcgcgaggcc ggcgcctggc
     3601 ccttgcgcac ccggtcgaag gcgctttcgc aatgatgggt gaagcgggcc agcgcatcga
     3661 agccgaacat cgcgcccgag cccttcagcg tgtgcagccc gcgaaagacc tcgtcgatca
     3721 gcccgcgatg ggccggcgcg ccggtcaggt cgagcagggc gcgctcgacc tggtcgagca
     3781 gttcacccgc ctcgatgcgg aaggcggcta ccggatcggc gccatcgctc accggcccag
     3841 caccttgcgg gccactttca ccagctggtc ggcgttgaag ggcttcacca gccagccggt
     3901 ggccccggcc gccttggcct cggccttcag cccgtcctcg ctttcggtgg tgaggaagac
     3961 gatcggcacg ccggtctggg tgggcgattt gcgcagctcg cggatcatcg tcagcccatc
     4021 catgcgcggc atgttgagat cggtgatgat gagatcgaag cgctgggtgc cggccttgct
     4081 gagcccttcc atgccatcgc cggcctccac cacattgtat ccggcccctg tcagcgcgat
     4141 gcgcacggcc atgcgcaggc tgggggaatc atcaacggtg agaatagagg cggtcatgca
     4201 cactctccgg gcagccagga tgcgacatct tgcgcagcag cagcattcag cccggcgcgc
     4261 gccagcagat cggccagcag cggatgggcg gggctttcca gcagcaggcg ccggccgcgc
     4321 cggcaggcct cggcttgcgc cgcgaccaac agctgaagga ggctaaaatc ggcctgcacc
     4381 agatcggccg ttgcgacgca gatgtcgtcg gccgcgccat caagggcggc cagcagctcg
     4441 gtgtggagcg cgcagatgtt ctggatggtc gcctgagcgg gaagcgtaaa agtgggcatg
     4501 aacctgtgct cgcgatgtgt tgcgcagcac ttagccgatg atcgcctgac gacttcgatc
     4561 cgggcgctta agtgttttca caaaagcccg gcaagggggg gcaaggaagg ggggaggggg
     4621 ggcacctgcc tgcaggcgcg gggcccgtca ggcggcgtgg cggcgctgct gggccagcca
     4681 ggcgcgggcg cggcgctggg cctcggcgat ctcgcgcgcg ctcatgtcct cggccacttc
     4741 ggcgcgggcc gcgggggctt cctcatggcc atgggcggcg gccaggttaa accatttgtg
     4801 cgcttccacc agatcgcagg gcgcgccatg atcgccggtg gaaaaggcca cgcccaggct
     4861 gaagcaggca tcggcctcgc cctgttcggc agcggccagc caatgggcca tcggcagggc
     4921 cgggcccatg ctggcggcca tcgcatttcc tgcaaacggg cccggcgttg cgcccaggcc
     4981 ttcagatcct ctgatcatcg ccaattcctt cgtgccgggc tgccctgcgg gcggccattg
     5041 gcgtcatgac tcgccgatca tggtcaatat aagattaacg atgtagtgtt ctcttgcggg
     5101 acaggatgaa gcctccgggc gtttccttag tcattcgtgc ttgtatggca caggccgcgc
     5161 cactataggc cagcctgccc gttgccgggc ttgtgcccga taagtgccgc cgggcgcggt
     5221 cttttggggg gcaggttccg gctttagtcg gaggggttga tggccaacgg tttggcagat
     5281 gatatcgacg ttctggtaaa ggcaaggcgc tcgctggcgg gcgattatgt ccccactgac
     5341 gatgaaacct acatgagcga gccgcagctg gattatttcc gccgcctgct gcagcagtgg
     5401 aaaaagctga ttctctccgc cgcggccgac acgctcagcc agttgcagga aggcccgctg
     5461 cgcgagccgg acctgaatga ccgcgcgtcc tcggagaccg attggggcat cgagctgcgc
     5521 acgcgtgatc gccagcgcaa gctgatcgcc aagatcgatt cggccctgcg ccgcatcgag
     5581 gaaggcgaat atggcttttg cgaagtgacc ggcgagccga tcggccttgg ccggctggtt
     5641 gcccgcccca tcgccacgat gacggtggaa gcccaggaag cccatgaacg ccgggagaaa
     5701 atctcgcgcg acgattgagc gctgttgccg gcgcgggcgg caggcctgcc agcgccggca
     5761 agagattgtt aagtgcttta ccttagttta cagtcttgcg cggaggctgt tgttccgtgc
     5821 tgtcagcaat ccggataaga accgagtagg ccggcccatg agcgattccg atcaacgcct
     5881 tcagccgcga gacagcctgt tcctgctggc ccggctgcgc cttgtcggcg atgtggcgga
     5941 atggcgggtg aaggtgcgca acctgtcggc cagcgggctg atggccgagg gcgatatcga
     6001 tatccagcgc ggcactgccg tgcagatcga actgcgcaat ctgggctggg tcaacgggct
     6061 ggtggcctgg gtgcagggca atcgcctcgg cgtggcgctg gagcaggaga tcgatcccaa
     6121 atcggtgcgc gcccagcccg atgccaccgc cgccgacgag cgcgcctatt atcaccgccc
     6181 ctcgctgacg gccgccgcgc aaacccacgc gcccgatccg cgccgcctgc gcaagatcta
     6241 gcccgccgcg agaaaccgac cctccgcgag aggccggttg ccgcgccggc ccaaccccat
     6301 taggctgtgg ccgatgcttg ccggcgacac gctttccctt ctcccgctgg agcggctgcc
     6361 ccctctcccg ccgaagcgac agggcctgtg gcgcgccccg gcgctgctgg gcatctgcgc
     6421 gctgctggcc gcttgcggcc atggccaggg ccagacggtg gatgtggcgg tgatcggcgc
     6481 gccggccagc ctgtttgccg cgccggccag cccgcctgcc gcggctagcg ccgaccagca
     6541 cctgctccag cacctgctgc tgtcggccac gcgcgagggg ctggtggctt tcgacgagga
     6601 aggcaagatc gtgccggcca ttgccgatcg ctggatcgtg gccgacgatg ggcaaagctt
     6661 catcttccgc ctgcgcgatg gcacctggcc ggatggcgcg gggctgaccg gcgattcggt
     6721 gcgccaggcg ctgcatgccg cgatagaggc cgcccgcaag gggcccttgg ccggcgatct
     6781 ggcgatgatc accgatgtgc gggcgatggc cggccgggtg gtggaaatcc gcctgaccca
     6841 gccgatgccc gatttcctgc aactgctggc ccagcccgaa ctgggggtga tgcaccacgg
     6901 caagggcacc gggccgatgc tgctgcaccg cgagggcgat gcggccgtgc tggtgcccat
     6961 cgcgccggaa aagcgcggcg cgccggcggt ggaaggctgg gccgacatgg cgcatgacct
     7021 gcgcctgcgg gcgctgccgg ccgatgcggc gctggccgaa ctggccgccg gccatgtgca
     7081 ggtggtactg ggcggcggct ttgccgatct gccacggctg cgcgcgcgcg cgctcgatca
     7141 gcggcagttg cggctggatc cggtgagcgg cctgtttggc ctggccgtgg ccgataccgg
     7201 cggcctgctg gccacgcccc agctgcgcga ggcgattgcc atggcgatcg accgcgattc
     7261 gctggccacc acgatcgcgc cggctggctg ggccgccagc agccgggtgc tgcccaccgg
     7321 ccttggcagc gaatcgctgg ccacggccga acgttggtcc gatatcgatc tggatggccg
     7381 gcgcacgctg gcgctgcggc gaatcgccct gtgggcgggc gcggcgcacc agcatccggt
     7441 gctgcgcatc gccctgccgg aagggccggg cgcggatctg ctgttcgacc ggctgagcaa
     7501 tgatctggcc gatgtggaag tgggcgcgca gcgcgtgggc atggatcagc cggccgatct
     7561 gcggctggtc gacgaagtgg cgccatatgc caggttaagc tggtttttca gcaggttgtc
     7621 ttgcagggtg aggggccctg gctgcgcccc ccaggccgat gcgctggccc agcaggcagc
     7681 gctcgccgct gatccggcca gcgcccgggc gctgtatggc gaggccgggc ggcaactggc
     7741 agaagccaat gttttcattc ctttcggccc gccgatccgg tggtctgcgg tggccggggg
     7801 caccaccggc tttgcgatta accgtatcgc tgtgcatccg ctgctgccgc tggcgattcg
     7861 cccgcgctag caggcacaat cctgccggcc cggcggcaaa aaaccgaccg ccggacaatt
     7921 tcatccaaaa ttaacctttc gccttcagtg ttgctatggt taatagtgga ttttgagccc
     7981 ggattgggcc ctatctgcta tgcggggcaa aaggggattt acccatgcgc gtgttgttga
     8041 tcgaggatga accggccacg gcaagggcta tcgagctcat gcttaccacc gagggcttca
     8101 atgtatattc caccgatctg ggggaagaag gcctcgatct tggcaagctc tacgattacg
     8161 atatcatcct tctcgatctc aacctgcccg atatgcatgg ctatgatgtg ctgaagaagc
     8221 tgcgcgtcgc caaggtgcag acgccggtgc ttattctgtc gggcatctcg gaaatggatt
     8281 cgaaagtgcg cagcttcggc ttcggcgctg acgactacgt gaccaagcct ttccaccgcg
     8341 aagagctgat cgcccgcatc cacgccgtgg tgcgccgctc gaagggccat tcgcaatcgg
     8401 tcatccgcac tggcaagctg tgcgtcaacc tcgatgcgaa gacggtggaa gtggatagcg
     8461 cgcgcgtcca tctcaccggc aaggaatatg ccatgctgga gctgctgagc ctgcgcaagg
     8521 gcaccacgct caccaaggaa atgttcctca accacctcta tggcgggatg gacgagcccg
     8581 agctcaagat catcgacgtg ttcatctgca agctgcgcaa gaagctggcc catgcctgcg
     8641 gcggcgagaa ctacatcgag acggtgtggg gccgcggcta tgtgctgcgc gatcccgatg
     8701 aggctgcctt cgccgcctga ccagcgccgg cagacccaat gcaaaggccc gcccctgccc
     8761 cggcgggcct ttgccgtttg ggggggaggc aagggctgca cccctgccgc aatccccccc
     8821 taaaacctca tttgcgccga taacggaaat accacacctc atgcccgtag acattgcggg
     8881 ccttggcctc atagcgcgtt tccggccagc ccgaggggcg gttctggaaa tcgctggcgg
     8941 tgctggccag ccattcgaac tggtgggtgt ggcgctgcat caccatcagc gcgtggcgca
     9001 gatagatggc atggtcggtg ccgaagcgca gctcgccgcc cggcttcagc ttggcggcaa
     9061 acaggtccac cggcccgtcg ttcatcatcc ggcgcttggc gtggcgggcc ttgggccagg
     9121 ggtccgggtg aagcaggtag aggaacgaca gcgccccgtc ggggatgcgg cgcagcacct
     9181 ccagtgcatc gccatggtgg atgcgcacat tgcccagcgg cgggtgctgg ccgttgtcgc
     9241 ccgccacatg ggtcagcgcc tgggccacgc cgttgaggaa aggctcggcg ccgatgaagc
     9301 cgtggccggg cagcatgtcg gcgcggaagg ccatatgctc gccgccgcca aagccgatct
     9361 cgaaatggag cgggcaggcc tcgccaaaca ggccttcggc agtcacgggc ccttcggcgg
     9421 gcacggcaat gcgcggcagc aggtcatcca ccagcgcctg ttgcccggcg cgcaggggtt
     9481 tgcccttggc gcggccgtag agccggttga gcgtggtggg atcgccgtct ttatgtgccg
     9541 tcatggcgcc ggcggatggc aggcgcgggc gcgggggtca agtgggctgg ggccgggccg
     9601 ggcggaacca atgcgcgcgc cggcgattgt gtgcgcaagc ggcgttgcgc cggctcaagg
     9661 aggtgtgatg atcgtttttg gctgcctggg gctgatcgtg ctgccgctgc tgggcttttt
     9721 tgccggcctg tggctgggcg ggccgctggt gggggcctgg tgcgcgggcg cgggctttgt
     9781 gctggccgca gcgctgtcgg tggtgatgac ctatgcgatg gtgaaggcca gagcctgacc
     9841 gcaagggcgc cgggcggggc gaggggccat ccccccgccc cgcccgggcg ccgcttcagc
     9901 ctgccacttc ggccgccgcc agcaccgcca gcgtcagcac atcgggggcg atggcggtca
     9961 tcggcacgat ctgcaccggc ttttccatgc ccaccagcat cggcccgatc gccgtgttgc
    10021 cgcccagttc gcgcagcagc ttggccgcga tattggccga ttgcaggccg gggaagatca
    10081 acacattggc cggggccgtg aggcgcgaga agggatagag cttcatcacc gtggggttga
    10141 gcgcggcatc gggggccacc tcgccctcat attcgaagcc cggctgctcc tcgtcgagga
    10201 tggcgatggc gccgcgcagc ggctccagcc agcggccctg cggattgccg aaagtggaat
    10261 aggagaggaa ggccacccgc ggctcatggc ccaggcgccg ggccacagcg gcggtttcct
    10321 tggcgatcac cgccaggtct tcggcggtgg ggcgttcgtt gcaggtggtg tcggccagga
    10381 acacggtggt gttcttgccc accatcacat ggatgccgaa aggcaggtgg ccgggcttgg
    10441 gatccagcac atggcgcact tccttcatcg tctgcgcgaa ggggcgggtg atgccggtga
    10501 ccagcgcatc gcccacgccc agcttcagca gcgccgaggc aaaggcgttg cgatcctggt
    10561 tgaccatgcg cttgatgtcg cgctccagat agccgcggcg ctgcaggcgg tcatagagca
    10621 tgcggcccat cgcttccaca tgcacgctgt cggcggcatt gtggatttcc cagccatcgg
    10681 ggttgcccac gcccagttcc accagcttgt cgcgcacggc ctgggtgcgg cccaccagca
    10741 ccggcgtgcc atagccgaaa tcgcggaact ggatcgcggc gcgcagcacc acctcgtttt
    10801 ccgcctcggc gaagatcacc cgcttggggt tctccttcac ctgggcatac acttgcgtga
    10861 gcaccgaggt ggtggggttg aggcgggcct tcagctgatg gcgataggcg tcgaaatcct
    10921 cgatcggctt ttgcgccacg ccggtttcca tcgccgcctt ggcaacggcg gaaggcacga
    10981 tctccatcag ccgcggatcg aacggggcgg ggatgatgta atcgcgcccg aagctgtggt
    11041 tcctgccata ggccagcgcc acttcctcgg gcacctgctg gcgcgccagt tcggcaatgg
    11101 cattggccgc ggcgatcttc atctcttcgt tgatggccgt ggcccgcaca tccagcgcgc
    11161 cgcggaagat gaaggggaag cccagcacat tgttgacctg gttgggataa tccgaccggc
    11221 cggtggcgat gatggcatcg ggccgggcag cgcgcgcctc gggcggggtg atctcggggt
    11281 cagggttggc catggcaaag atgatcggct ctgcggccat cttcttcaca tagtccgcct
    11341 tcagcgcgcc ggcggcggaa aggccaatga acacatcggc ccccaccagc gcctcttcca
    11401 gcgtgcgggc gggggtgtcc accgcatggg cgctcttcca ctggtccatg ccggtttcgc
    11461 ggccgcgcca gatggtgccc tgccggtcgc agacgaccac attctgatgc ggcacgccca
    11521 tgctcttgat cagcgcggtg caggccagcg ccgaagcgcc ggcgccgttc accaccacct
    11581 tgatgtcctt cagctcgcgg ttggtgagca ggcaggcgtt gatgaggccg gccgcggcga
    11641 tgatcgcggt gccatgctgg tcgtcgtgca tgacagggat cttcatgcgc tcgcgcaggg
    11701 cctgctcgat gacgaagcat tcgggcgcct tgatgtcttc aaggttgatg ccgccaaagc
    11761 tgggctccat cagcgcgatc gcctcgatca gcgcctcggg gtcttcgctg gccatctcga
    11821 tgtcgatcga atcgacatcg gcaaagcgct tgaacagcac cgccttgcct tccatcaccg
    11881 gcttggaggc cagcgcgccc agattgccca tgcccagaat ggccgtgccg ttcgagatga
    11941 cggcaaccag attgccgcgc gcggtatagt cgtaggcggt ggcgggattg tcggcgatgg
    12001 cctgcaccgg cactgccacg cccggcgaat aggcgaggct gagatcgcgc tgggtcgcca
    12061 tcggcttgga ggcgatgatc tcgattttgc ccgggcggat ggtgttatgg tagaacagcg
    12121 cctcgcgctc ggtgaaacga acgttgcttt cctcggacac caaaactttc tccttctttg
    12181 ccccagccct aaccacaatg cgaggcccgc gataggggaa agcgtgggat ggtgccattc
    12241 cctgtcatcg atttcgcggc taaccccgtg ggcatgagcg atgcccccac cccgatgatg
    12301 agccaatatc ttgcgctgaa ggcagaggcc ggcaacagcc tgctgttcta ccgcatgggc
    12361 gactttttcg agctgttttt cgaggatgcg cgcctggccg cgcagatcct cgatattgcc
    12421 ctcaccagcc ggggcgaaca tgccggcgcc cccatcccga tgtgcggcgt gccggtgcat
    12481 gcggccgaga attacctggc ccgcctcatt cgcgccggtt gccgggtggc aattgccgaa
    12541 caggtggaaa cgcccgagga agccaagaag cgcggcggct ccaaggcgct agtgcggcgc
    12601 gccatcgtcc gcttcgtgac ggccggcacg ctgaccgagg aagccctgct ggagccgcgc
    12661 cgggccaatg tgctggcggc cctgtgcgag ctgcgcgggg cgatcggcat tgcctcggtc
    12721 gatatttcca ccggccggat ggagctggag gaatgcgccc ccgagcagct gggggcagcg
    12781 ctggcccgca ttggcgccag cgaactggtg gcgagcgacg gctgggatgc cggcccgctg
    12841 gaagccaccc cccgcccggc cgccgatttt gccagcgagg cgggcacgca gcgcctgtgc
    12901 gcgctgcatg gcgtgggcac gctcgacggg tttggcagtt tcagccgggc gatgctggcg
    12961 gccgcggccg ggcttgtcgc ctatctcgat catgtcgggc ggggcagcct gccgctgctg
    13021 ctgccgccgg tggcgcgcgt tcacgccggg catctcgcca tggacgaggc gacgcgcgcg
    13081 agcctcgaaa tcctgcaatc ggccgccggc gcgcgggaag gcagcctggt cgccacggtg
    13141 gaccgctgcg ttaccggccc cggcgcgcgc cagctggccg aggatctggc cgccccgctg
    13201 atggatcgcg ccgccatcgg ggcgcggctg gcgctggtgg gctggctgca tgaagaggcg
    13261 ctgctgcgcg aggatctgcg cgcggccttg cgcggcctgc ccgatgtggg ccgggcgctg
    13321 gggcgggtgg tggccgggcg cggctccccg cgagatctgg ggcagctgcg cgatgggctg
    13381 gcgggggcgg caaggctggc cgccctgctg gccgcgcaaa ccgcgcgccc ggcgctggca
    13441 gaggcgctgc tgcccacgct ggaaggccat gagccgctga tcgagctgtt cacccgcgcc
    13501 cttgtcgcca cgccccccac agagcgcggg cagggcggtt tcatcgccgc cggctatgat
    13561 gccgcgctgg atgaactgcg cgccatctcg cgcgatgccc gcgtggcgat cgccgcgctg
    13621 gaagcgaaat accgcgagga gaccggcatt gccgcgctca agatcaagca caatggcgtg
    13681 ctgggctatt tcatcgaagt gcccgcggcc cgggccgatg cgctgctggc ggccggcagc
    13741 ggcttcaccc accgccagac gatggccggc gccatgcgct tc
//
LOCUS       DASRYE010000309        11338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_187843,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000309 DASRYE010000000
VERSION     DASRYE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187843"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFF98_15810"
     CDS             complement(<1..869)
                     /locus_tag="VFF98_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZU65153.1"
                     /translation="MTALVWVEQDGAALKDATLAAVTAAGQLGGEVHLLVAGAGVAGV
                     AAQAAQIAGVAKVLVADDAAYAHGLAENVAPLVAGLMGGYDAFVATATAGAKNIAPRV
                     AALIDVAQLSEILSVEGPKTFTRPTYAGNAIATVESTDAKLVITVRGTAFAKAAAEGG
                     SAAIEAIAGAGDAGLSSFLGAEIAKSERPELTSAKIIVSGGRALKDAATFQATIFPLA
                     DKLGAAVGASRAAVDAGYVPNDYQVGQTGKIVAPEVYVAVGISGAIQHLAGMKDSKTI
                     IAINKDEDAPIFQV"
     gene            complement(1106..1852)
                     /locus_tag="VFF98_15815"
     CDS             complement(1106..1852)
                     /locus_tag="VFF98_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZU65154.1"
                     /translation="MKILVPVKRVIDYNVKPRVKADGTGVDLSNVKMSMNPFDEIAVE
                     EGIRLKEQGVASEVVVVSIGPAKAAETLRTALAMGADRAILISTDEEVEPLGVAKLLA
                     KVAGEETPGLVILGKQAIDDDSNQTGQMLAALLGRPQGTFASKVEVAGDHVQVTREVD
                     GGLETVRLALPAIVTTDLRLNEPRYASLPNIMKAKSKPLATKAPADYGVDVAPRLKVL
                     KVAEPPVRAAGIKVADVDALVGKLKELGVA"
     gene            complement(1897..2412)
                     /locus_tag="VFF98_15820"
     CDS             complement(1897..2412)
                     /locus_tag="VFF98_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZU65155.1"
                     /translation="MATSPPTADADLAVLLKRVLRFMPAPLGLVSCHDPLTDAPAGLA
                     MSAIMPVSLEPPAMAVAVNRSGAGHAALLRAGAFCINLLGPETAGQIGPFADPARRHE
                     RFADPAWRRNTHGWHLAGAPAAIFCRLRATLSHGTHDLLVGDVIALIARESAPVLGWN
                     DGAPALMQALG"
     gene            complement(2478..3557)
                     /locus_tag="VFF98_15825"
     CDS             complement(2478..3557)
                     /locus_tag="VFF98_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU65156.1"
                     /translation="MAKTEDYKLGEFTYPRGWFMIAAAEEASQTPVALRFFGQDMVLY
                     RGQASGKPILLEAYCPHMGTHLAKNTTSYVVTDRKHVEGDNIRCPYHAWRFGADGQCN
                     EIPYSPAPIPKRACVKSWPVVERAGALWIWHDEEGAEPDYDLPDFADHGADQWVPWTL
                     RCLGELDCHPQEIVDNMTDKAHLEPVHGTIEMMRFENEFDGITVRQILLGGHKTLAGA
                     EPMLNDTWYTGPGILQSIMVGEYPSLMLIAHTPVDDGRIKVWYGLMVKVAHATPTPED
                     IATARGYEDAGVYAFAQDFEIWGNKRACVHPMIVRGDGPFDKLRIWYRQFYNPRARAE
                     EFQKRVNGRYVAQGTTEAPWENTAA"
     gene            complement(3682..4185)
                     /locus_tag="VFF98_15830"
     CDS             complement(3682..4185)
                     /locus_tag="VFF98_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU65157.1"
                     /translation="MSAPQLDELDRQLIALLARDARVSNRKIAAELNVTEGTVRGRIR
                     RLQQDGLIAFTAITGYGVAEKTRLAFINILAEPGRLRAVSQEIAAWPEVNAVLITTGP
                     FNLVAMCLFGELDEVFALASDRIVAIPGVQQVETSVAVRTLSYNAGISRITAERQAEE
                     LGQGNDA"
     gene            4525..6210
                     /locus_tag="VFF98_15835"
     CDS             4525..6210
                     /locus_tag="VFF98_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65158.1"
                     /translation="MTEAWDRQTDILVVGSGAGGMLCALVGAEAGADVLIVEKAPLWG
                     GTSATSGAGIWIPGSSMARQAGFEDDLEAAFTYVRGLSAANVPDANIRAFVENAAPML
                     DWLVAHTPVQYMAFPYPDYHAENPGGSPTGFRTHMPLPLDGRRLGRDVRSQRFPSPAA
                     SLFGYINWHFDETYALLYRAKGWPWLLARRLAAYWLDLPFRFTSRKDRRLTLGNALTG
                     GLRMALNRRGVPLWLNTGLVELVEENGRVTGAVVMRQGQRLRIGARRGVVLAAGGFDK
                     NGQMRAAYAPFYPSAERSGGVSSNTGDAIRAGQALGARTLNLQSAWAAPVFHVPGEDR
                     GRLCTIERALPGCIMVDQRGERYLNEAASYHVVGQQMARRQQQAGNASPSWMIFDHRY
                     RHAYAMGPVYPLIPDWLHRRAIRSTLHKARSIGELGARIGVDPVRLAATVARFNRFAA
                     NGEDPDFHRGEAAYDRMYGDPRHGPNPCLRPIEQGPFYAMPIYPGDIGTNGGLLTDAE
                     AQVIGADGAPVAGLFAIGNSAASAMGESYPGAGVTIGPAMTFGYIAARRMMGA"
     gene            6245..6985
                     /locus_tag="VFF98_15840"
     CDS             6245..6985
                     /locus_tag="VFF98_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65159.1"
                     /translation="MLFAGKKVLVTGAASGIGRATAIRFAAEGAEVTIGDINAAGLAE
                     TAAHMARPPRQQLFDAADAASCQALVAAAATEGLDVLCNISGILQWGPSEEFAVADFA
                     RVLAINTTSVFALCQAALPHLLASGGNIVNTASTAGLQGTAYSVAYAASKHAVVGLTK
                     SLAVEFAARGVRVNAVCPGQVRTPMTQGRPPLGEIDWALVMRNAPKLADGICAPEDVA
                     EMFAFLASARAGKITGAVFAVDGGQLAG"
     gene            complement(6939..7442)
                     /locus_tag="VFF98_15845"
     CDS             complement(6939..7442)
                     /locus_tag="VFF98_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65160.1"
                     /translation="MADPALAALAARLARLEDLEAIRALIAAYGPLADAGDGARVAAL
                     FAPDGIYAIAGFAEARGRAAIAALLETPHHRALMADGCAHLLGPVAICLDGDTATACG
                     HSIVFKSGASGFAAHRVAANRWHLSRRAGQWLVARRDNALLDGNAAARILLSPPAAPH
                     PPQTPPR"
     gene            complement(7435..8904)
                     /locus_tag="VFF98_15850"
     CDS             complement(7435..8904)
                     /locus_tag="VFF98_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65161.1"
                     /translation="MTGLSMHRPLPAAQVPQWDFETDVAILGFGAAGASAAIEAAEAG
                     AQVMLCERNSGSGGASALSGGEIYIGGGTDAQRAAGFEDSAEDLAAYLKAAGGPCADT
                     AKCDLYARQSLAHYDWLKAQGVPYRGNYLPGKHIEPTDDSTLIWSGSEAAAPFCDLAR
                     PAPRGHVIAHMGWGGGRPLVDILEARARALGVAIHCDARGLALIEEAGAIVGLVVRID
                     NRPCFVKARRGVVLATGGFAMNEAMRRKYCPDTLRLSSPIGDKDDGGGILLGLGAGGE
                     AIHMEQFFSTCPWTMPEAHAHGVFVNRAGQRFINEDCYHGRVSRVALDQPGEQVYLLL
                     DNAHFSQPLDLAGITIAGTGADWAEVEGELGMAAGTLSATMAFYNAHAREGRDPLFGK
                     RPPILTALDQGPFIALELHFATSYFSFFTLGGLHTSCDGEVLDREGRPIAGLFAAGRC
                     TSGLPAWGHGYASGLSLADCTFFGRQAGRSAAQQARAHG"
     gene            complement(8916..10127)
                     /locus_tag="VFF98_15855"
     CDS             complement(8916..10127)
                     /locus_tag="VFF98_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU65162.1"
                     /translation="MATAATSHHTHRAPAPDDLIARAHAMIPTLKERARACTAGRDVP
                     AQTIADLHEAGFFRILQPKRWGGFEMHPNTFFAVQRALAEGCMSTGWMFGVVGCHPYE
                     LALFDERAQAEVWAENSATLASSSYQPVGKVTPVEGGFRLSGRWGFSTGSTHCQWVIL
                     GAMVPPAQPGAAADMRAFLLPRADYAIDTDAWHVFGLQGTGSHDIIVEDVFVPEYRTH
                     RAIDGFLCDNPGQKTNPAPLYTLPWAQVFMRSVSTAAFGGARAAITAAIDIAQSRVST
                     NTGKASKDDPFLMAAIARAHAQVTEMEHTLRLTFDDLMDLARQRLPIPMAKRAHYAFN
                     SASVVRRMADLVDDIVQLLGGRAIYTSSPIIQPWLDLNAGRAHVANDPNNRASDLVNT
                     ALGHQPSFNFL"
     gene            10320..11069
                     /locus_tag="VFF98_15860"
     CDS             10320..11069
                     /locus_tag="VFF98_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65163.1"
                     /translation="MDFAGRVAIVTGGTRGLGAGVARRLAAAGFAVHVCGRSQPETGP
                     QGIRFSAVDVRDAEAARAFVDSVASAHGRLDLLVNNAGGSPQASALASGPRFADAILR
                     LNLLAPLYMAQAAHPHMRASGGGSIVNIASVSAIRPSPGTAVYGAAKAGLVSLTQSLA
                     QEWGPDGIRVNAIIAGLMATETAGETYGDATAQAAVAASMPLGRMGTGEDIAGAVLWL
                     ASGLSGWVSGARINVDGGGERPYFLDLVKGG"
     gene            11074..>11338
                     /locus_tag="VFF98_15865"
     CDS             11074..>11338
                     /locus_tag="VFF98_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HZU65164.1"
                     /translation="MGVKALGYVVIATARLEEWDRFLTEVVGVMRGGPAADGGALYRI
                     DGRPFRFWLQPAAQEALAASGYEIADAEGWAALRARIVAAGRPV"
BASE COUNT         1755 a         3625 c         4055 g         1903 t
ORIGIN      
        1 acctggaaga tgggtgcgtc ttcgtccttg ttgatggcga tgatggtttt gctgtccttc
       61 atgccggcga ggtgctggat ggcgccggaa atgccgacgg ccacatagac ttcaggcgcg
      121 acgatcttgc cggtctggcc gacctgatag tcgttcggca catagccggc atcgacggcg
      181 gcgcggctgg cgccaacggc cgcgcccagc ttgtcggcta gcgggaagat ggtggcctgg
      241 aaagtggccg catccttcag cgcccgcccg cccgagacga tgatcttggc cgacgtcagc
      301 tcagggcgtt ccgacttggc gatctcggcg ccaaggaagc tggaaaggcc ggcatcgccc
      361 gcgcccgcaa tcgcctcgat ggcagcgctg ccaccctcgg ctgcggcctt ggcaaaagcg
      421 gtgccgcgca cggtgatgac gagcttggca tcggtgctct ccaccgtggc gatggcattg
      481 ccggcatagg tcggccgggt gaaggtcttg gggccctcca cgctgaggat ctccgacagc
      541 tgcgccacat cgatcagcgc ggccacgcgc ggggcaatgt tcttggcgcc ggccgtggcg
      601 gtggcgacaa acgcgtcata gccgcccatc agcccggcca ccagcggcgc cacattctcg
      661 gccagcccat gggcataggc ggcatcatcg gccaccagca ccttggcgac gccggcaatc
      721 tgcgccgcct gcgcggcaac gccggcaacg cccgcgcccg ccaccagcag atgcacctcg
      781 ccgcccagct ggccggccgc ggttaccgcc gccagcgtcg catccttcag cgcggcgcca
      841 tcctgttcaa cccaaaccaa tgcggtcatt gttcgtgatc cttatttgcg agggaaggtc
      901 ggagaaggga gggaggggtg cgagggttat taccctcgcg ctcccgttat ctggccgcct
      961 tgcgctgcca tcgccatgag gcgcaaagcg gcgaagccgc agtgttcata gcctccggcg
     1021 gggcggcgtc gcgctttggg caccgccacc gcccatgtaa cgggagcgcg agggtgataa
     1081 ccctcgcaac cctcacctca ccccttcagg cgacccccag ttccttgagc ttgcccacca
     1141 gcgcatcgac atcggcgacc ttgatgccgg ccgcgcgcac cggcggctcg gccaccttca
     1201 gcaccttgag ccgcggcgcc acatcgaccc cgtagtcggc cggcgccttg gtggcgagcg
     1261 gcttggactt cgccttcatg atgttcggca gcgaggcata gcgcggctcg ttcaggcgca
     1321 ggtccgtcgt cacgatcgcc ggcagcgcca ggcgcaccgt ctccagcccg ccatccactt
     1381 cgcgcgtcac ctgcacgtgg tcgccggcca cttccacctt gctggcaaag gtgccctgcg
     1441 ggcgccccag cagcgcggcc agcatctgcc cggtctggtt gctgtcatcg tcgatcgcct
     1501 gcttgcccag gatcaccagg cccggcgttt cctcgccagc caccttcgcc agcagcttcg
     1561 ccacgcccag cggctccact tcttcatcgg tgctgatcaa aatcgcccgg tccgcgccca
     1621 tcgccagcgc cgtgcgcagc gtctcggccg ccttcgcagg cccgatcgac accaccacaa
     1681 cctccgacgc caccccctgt tccttcaggc ggatcccttc ttcaaccgcg atctcgtcaa
     1741 acgggttcat gctcatcttc acgttcgaca ggtccacacc cgtcccgtcc gccttcactc
     1801 gcggcttcac gttgtagtct atcacacgct tcaccggcac cagtatcttc atgaatctcc
     1861 ctttcgaatc cggttgtcgt tggcgatcgg ccgggctcag cccagagctt gcatgagggc
     1921 cggggcgccg tcgttccagc cgagaacagg ggcgctctcc cgggcgatga gcgcgatgac
     1981 atcgccgacc agcagatcgt gggtgccatg ggaaagggtt gcgcgcaatc tgcagaagat
     2041 cgccgccggc gcgccggcca ggtgccagcc atgggtattg cggcgccagg cggggtcggc
     2101 aaagcgttcg tggcggcggg cgggatcggc aaaggggccg atctgcccgg ccgtttccgg
     2161 gcccagcagg ttgatgcaaa aggcgcccgc gcgcagcagc gcggcatggc ccgcacccga
     2221 gcggtttacc gcaacggcca tggccggcgg ttccagcgag accggcatga ttgccgacat
     2281 ggccagcccg gcaggcgcgt cggtcaaggg atcgtggcag ctgacgaggc cgagcggggc
     2341 cggcatgaaa cgcagcacgc gcttcagcag aacggccaga tcagcatctg ccgtgggcgg
     2401 ggaagtggcc atgagagggg caatggtcct gtgagggcac gggcgcgccg gcgggcgggc
     2461 gcccgtgcgg gaagggatca ggcggcggtg ttttcccagg gcgcctcggt ggtgccctgg
     2521 gccacatagc ggccgttcac gcgcttctgg aactcctcgg cgcgcgcgcg cggattgtag
     2581 aactggcgat accagatgcg cagcttgtcg aaggggccat cgccgcgcac gatcatcggg
     2641 tgcacgcagg cgcgcttgtt gccccagatc tcgaaatcct gggcaaaggc atagacgccg
     2701 gcatcctcat agccgcgggc ggtggcgatg tcttcgggcg tgggcgtggc atgggccacc
     2761 ttcaccatca gcccatacca caccttgatg cggccatcgt cgacgggcgt gtgggcgatg
     2821 agcatcagcg agggatattc gcccaccatg atcgattgca ggatgccggg ccctgtatac
     2881 caggtgtcgt tcagcatggg ttcggcgccg gccagggtct tgtggccacc cagcaggatc
     2941 tggcgcaccg tgatgccgtc gaactcattt tcgaagcgca tcatctcgat ggtgccgtgc
     3001 accggttcga gatgggcctt gtcggtcatg ttgtcgacga tttcctgcgg gtggcagtcc
     3061 agctcgccca ggcagcgcaa ggtccacggc acccactgat cagcgccatg atcggcgaaa
     3121 tcgggcaggt cgtagtcggg ctctgcgcct tcctcgtcgt gccagatcca cagggcgccg
     3181 gcgcgttcca ccaccggcca gctcttcacg caggcgcgct tggggatggg cgcgggcgaa
     3241 taggggatct cgttgcactg gccatcggcg ccaaagcgcc aggcgtgata ggggcagcgg
     3301 atattgtcgc cttcgacatg cttcctgtcg gtcaccacat aggaggtggt atttttggcg
     3361 aggtgggtgc ccatatgcgg gcaataggcc tccagcagga tcggcttgcc cgatgcctgg
     3421 ccgcgataga gcaccatgtc ctggccgaaa aagcgcaggg cgacgggggt ttggctggcc
     3481 tcttcggcag cggcgatcat gaaccagccg cgcgggtagg tgaattcgcc cagcttgtaa
     3541 tcttcagtct tggccatttt cgggaatcct ccggtttccc gccagcatag atcatgtcgt
     3601 aggtgttcgg aagatggatt ccgacaaact caatttatgt cggtaaaaat ggcattcatg
     3661 cgtaaaattt ctcatacatt tctacgcatc attcccctgc cccagctcct cggcctggcg
     3721 ctcggcggtg atgcgcgaga tgccggcatt gtagctgagc gtgcgcaccg ccaccgaggt
     3781 ttccacctgc tgcacgccgg ggatggccac gatccggtcg gaggccaggg caaacacctc
     3841 gtccagctcg ccaaacaggc acatcgccac caggttgaac ggcccggtgg tgatcagcac
     3901 ggcattcacc tcgggccagg cggcaatttc ctgcgagaca gcacgcagcc ggcccggttc
     3961 ggcgaggatg ttgatgaagg cgaggcgggt tttttccgca acgccatagc cggtgatggc
     4021 agtgaaggcg atcagcccat cctgctgcag gcggcggatg cggccccgca cggtgccctc
     4081 ggtgacgttc agctcggcgg cgatcttgcg gttggagacg cgcgcatcgc gggccagcag
     4141 ggcgatcagc tggcggtcga gctcgtcaag ctggggggca gacatgtgcg gttcggctcc
     4201 ttgcctcgcg ccggtttgcc tttgcggctg gccgggcgcg gggcccgggg cgcaatcgcg
     4261 ttgtcaaacc ggtgttttct tctgccatgc tagtgcgtgg gcgcccctgt ggcgtcaaca
     4321 ggaatatgcc gcggcggggg ctggtggcgt gccggcgatt tttctgaaaa tcgtattcca
     4381 gaaaaatata aattacgcat atttggcatt aatacccgat aaaatgcatt gtttgcgttg
     4441 tcgtatggcc ggtgctatcg ctgatcgcaa cagagcggga gcgcggccgg cacaccggcg
     4501 cgaggagcga ggaaaaggcg gtctatgaca gaggcgtggg acaggcagac ggatattctc
     4561 gtggtcggct cgggcgcggg gggcatgctg tgcgcgctgg tgggggcaga agcgggcgcc
     4621 gatgtgctga tcgtggaaaa ggcgccgctg tggggcggca cctcggccac atcgggcgcg
     4681 gggatctgga ttccgggcag cagcatggcc cgccaggccg gcttcgagga tgacctcgag
     4741 gcggccttca cctatgtgcg gggcctgtcg gcggccaatg tgcccgatgc caatatccgc
     4801 gcctttgtgg aaaatgccgc ccccatgctc gattggctgg tggcgcacac gccggtgcaa
     4861 tacatggcct tcccctatcc cgattatcat gccgaaaacc ccggcggatc gcccaccggt
     4921 tttcgcaccc acatgccgct gccgctcgac gggcggcggc tggggcgcga tgtgcgcagc
     4981 cagcgctttc cctcgccggc ggccagcctg ttcggctata tcaactggca cttcgacgag
     5041 acctatgcgc tgctctatcg cgccaagggc tggccatggc tgctggcgcg ccggctggcg
     5101 gcctattggc tggatctgcc cttccgcttc acctcgcgca aggatcgccg gttgacgctg
     5161 ggcaatgcgc tgaccggcgg gctgcgcatg gcgctgaacc ggcgcggggt gccgctgtgg
     5221 ctgaacacgg gcctggtgga gctggtggaa gagaacggcc gggtgacagg cgctgtggtg
     5281 atgcgccaag ggcagcggct gcgcattggc gcgcggcgcg gggtggtgct ggcagcgggc
     5341 ggcttcgaca agaacgggca gatgcgcgcg gcctatgcgc cgttctatcc cagcgccgag
     5401 cgttcgggcg gggtgagcag caacaccggc gatgccatcc gcgccgggca ggcgctgggc
     5461 gcgcgcacgc tgaacctgca atcggcctgg gcggcgccgg tgttccatgt gcccggcgag
     5521 gatcgcgggc ggctgtgcac gatcgagcgg gcgctgcccg gctgcatcat ggtcgaccag
     5581 cggggcgagc gctatctcaa cgaggccgcc tcctatcacg tggtgggcca gcagatggcc
     5641 cgccgccagc agcaggccgg caatgccagc cccagctgga tgatcttcga ccaccgctat
     5701 cgccatgcct atgcgatggg cccggtctat ccgctgatcc ccgactggct gcaccgccgc
     5761 gccatccgct cgaccctgca caaggcgcgc agcatcggtg aactgggcgc gcgcatcggc
     5821 gtggatccgg tgcggctggc ggccacggtg gcgcgtttca accgctttgc cgcgaacggc
     5881 gaggaccccg atttccaccg cggcgaggcc gcttacgatc gcatgtatgg cgatccgcgc
     5941 catgggccca acccctgcct caggccgatc gagcaggggc cgttctatgc catgccgatc
     6001 tatcccggcg atatcggcac caatggcggc ctgctgaccg atgccgaggc acaggtgatc
     6061 ggcgccgacg gggcgccggt ggcagggctc tttgccattg gcaacagcgc ggcctcggcc
     6121 atgggcgaaa gctatccggg cgccggcgtg acgatcggcc cggcgatgac ctttggctat
     6181 atcgccgcgc gccggatgat gggcgcctga gcgcccgcaa ccaccacact cagggggaga
     6241 gatcatgctg tttgccggaa agaaggtgct ggtcaccggt gccgcctcgg ggatcgggcg
     6301 ggccacggcc atccgctttg ccgccgaggg cgccgaggtg acgatcggcg acatcaacgc
     6361 cgccggcctt gccgaaaccg ccgcgcacat ggcccgcccg ccgcgccagc agcttttcga
     6421 cgcggccgat gcggcctcgt gccaggcgct ggtggcggcc gcggccacag aggggctgga
     6481 tgtgctgtgc aatatctcgg gcatcctgca atggggcccg tcggaagagt ttgccgttgc
     6541 cgattttgcc cgcgtgctgg cgatcaacac caccagcgtg tttgccctgt gccaggcggc
     6601 gctgccgcat ctgctggcct ctggcggcaa tatcgtcaac accgcttcca ccgccgggct
     6661 tcagggcacg gcctattcgg tggcctatgc cgcttccaaa catgcggtgg tgggccttac
     6721 caaaagcctg gcggtggaat ttgccgcgcg cggggtgcgg gtgaacgcgg tgtgcccggg
     6781 ccaggtgcgc acgccgatga cgcaaggccg cccgccgctg ggcgagatcg actgggcgct
     6841 ggtgatgcgc aatgccccca agctggccga tggcatctgc gcgcccgagg atgtcgccga
     6901 gatgttcgct ttcctggcca gcgcgcgggc cggcaagatc accggggcgg tgtttgcggt
     6961 ggatgggggg cagctggcgg gctgagcagg atccgcgctg ccgcgttccc atcgagcagc
     7021 gcgttgtcgc gccgggccac cagccactgg ccggcccggc gcgacaggtg ccagcggttg
     7081 gccgcaacgc gatgggcggc aaaaccgctg gcaccgcttt tgaagacgat gctgtggccg
     7141 caggcggtgg ccgtatcgcc atcgaggcag atcgccaccg gccccagcag atgggcgcag
     7201 ccatcggcca tcagcgcgcg gtgatggggc gtttccagca gggcggcaat ggcggctctg
     7261 ccgcgtgcct cggcaaagcc ggcgatggca tagatgccat cgggcgcgaa aagggcggca
     7321 acccttgcgc catcgcccgc atcggccagc gggccatagg cggcgatcag cgcgcggatc
     7381 gcttccagat cctccagccg ggccagccgc gcggccagcg ccgccagcgc cggatcagcc
     7441 atgcgcgcgc gcctgctggg ccgcgctgcg ccccgcctgc cggccgaaga aggtgcaatc
     7501 ggcaaggctc aggccagagg catagccatg cccccaggcc ggcaggccgc tggtgcagcg
     7561 cccggcggca aacaggccgg cgatgggccg cccctcgcgg tccagcactt cgccatcgca
     7621 gctggtgtgc aggccgccca gcgtgaagaa gctgaaatag ctggtggcaa aatgcagttc
     7681 cagcgcgatg aagggcccct ggtcgagcgc ggtgaggatc ggcgggcgct tgccgaacag
     7741 ggggtcgcgg ccctcgcggg catgggcgtt gtagaaggcc atggtggccg acagcgtgcc
     7801 cgcggccatg cccagctcgc cctccacctc tgcccaatcg gcgccggtgc cggcgatggt
     7861 gatgccggcc agatcgagcg gctggctgaa atgggcattg tcgagcagca gatagacctg
     7921 ctctccgggc tggtcgagcg ccacccggct gacccggccg tgatagcaat cctcgttgat
     7981 gaagcgctgc ccggcgcggt tgacgaaaac gccatgggca tgggcctcgg gcatggtcca
     8041 ggggcaggtg gagaagaact gctccatgtg gatcgcctcg ccgccggcgc ccagccccag
     8101 caggatgccc ccgccatcgt ccttgtcgcc gatggggctg gaaaggcgca gcgtgtcggg
     8161 gcaatatttg cggcgcatgg cctcgttcat ggcaaagccg ccggtggcca gcaccacgcc
     8221 gcgccgcgcc ttcacgaagc agggccggtt gtcgatccgc acgacaaggc cgacgatcgc
     8281 ccctgcctcc tcgatcagcg ccaggccgcg cgcatcgcaa tggatcgcca cccccagcgc
     8341 ccttgcccgc gcttccagaa tatcgaccag cggccgcccg ccgccccagc ccatatgggc
     8401 gatgacatgg ccgcgcgggg ccggccgggc gaggtcgcaa aagggcgctg ccgcctcgct
     8461 gcccgaccag atgagcgtgg aatcatcggt cggctcgata tgtttgccag gcagatagtt
     8521 gccgcgatag ggcactcctt gcgccttcag ccagtcgtaa tgggcgagcg actggcgggc
     8581 atagagatcg catttggcgg tgtcggcgca agggcccccg gcggccttca gataggcggc
     8641 cagatcctcg gcgctgtctt caaagccggc ggcgcgctgg gcatcggtgc cgccgccgat
     8701 atagatctcg ccgcccgaaa gggccgaggc gccgccgctg cccgaattgc gctcgcacag
     8761 catgacctgc gcgcccgcct ctgccgcctc gatcgccgcc gaggcgcccg ccgcgccaaa
     8821 gcccaggatc gccacatcgg tctcgaaatc ccactgcggc acctgcgcgg ccggcagggg
     8881 gcggtgcatg gacaggcctg tcatgtgttg ctgtctcaca ggaagttgaa gcttggttgg
     8941 tggccgagag cggtgttgac gaggtcgctt gctcggttgt tggggtcgtt ggcgacgtgt
     9001 gcgcgtccgg cgttgaggtc gagccagggc tggatgatgg ggcttgatgt gtagatggcg
     9061 cgcccgccca ggagctggac gatatcatcg acgagatcgg ccatgcggcg gacgacggaa
     9121 gcggagttga aggcgtagtg ggcgcgtttg gccatgggga tgggcaggcg ctggcgggca
     9181 aggtccatga ggtcgtcgaa ggtgaggcgc agggtgtgct ccatttcggt gacctgggcg
     9241 tgggcgcggg cgatggcggc catgaggaag ggatcgtcct tcgaggcctt gccggtgttg
     9301 gtggagacgc ggctctgggc gatgtcgatg gccgcggtga tggcggcgcg ggcgccgccg
     9361 aaggcagcgg tggagacaga gcgcatgaag acctgggccc atggcagggt gtagaggggg
     9421 gccgggttgg tcttctgtcc ggggttgtcg cacaggaagc cgtcgatggc gcggtgcgtg
     9481 cggtattcgg ggacgaagac atcctcgacg atgatgtcgt ggctaccggt gccctggagg
     9541 ccgaagacgt gccatgcgtc ggtgtcgatg gcgtagtcgg cgcggggcag gaggaaggcg
     9601 cgcatgtcgg ctgcggcgcc tggctgggcg gggggcacca tggcgcccag gatcacccat
     9661 tggcaatggg tcgatccggt ggagaagccc catcggccgg agaggcggaa gccgccctcg
     9721 acaggggtga ccttgccgac gggctggtag gaggaggagg cgagggtggc gctgttttcg
     9781 gcccagacct cggcctgggc gcgctcgtcg aagagggcca gctcgtaggg gtggcagccg
     9841 acgacgccga acatccagcc ggtggacatg cacccttcgg ccagggcgcg ctggacggcg
     9901 aagaaggtgt tggggtgcat ttcgaagccg ccccagcgct tgggctggag gatgcggaag
     9961 aagccggctt cgtggagatc ggcgatggtt tgggcgggga catcgcgccc tgcggtgcag
    10021 gcgcgggcgc gttccttgag ggttgggatc atggcgtggg cgcgggcgat gagatcatcg
    10081 ggcgcggggg cgcggtgtgt gtggtgcgat gtggcggcgg tagccatgat cagggcctct
    10141 tccgtttgtg gcgtggccgg cgtttagccg atgctcgtaa aaaaatatgc caacagatac
    10201 gcaaatggtc aattgaaaag cttgtcggct ttggatagtg atgttttcga atctggcgat
    10261 cgtggcaaat ccgcgtcgcg caaaaaatgg gagagcgcgg cttggcggcc gatggcatca
    10321 tggatttcgc aggccgtgtg gcgatcgtca ccggcggcac gcgcgggctg ggcgccgggg
    10381 tggcccggcg gctggcggcg gccggctttg ccgtgcatgt gtgcgggcgc agccagcctg
    10441 aaacagggcc gcaagggatc cgcttttccg ccgtggatgt gcgcgatgcc gaggccgcgc
    10501 gcgcctttgt cgacagcgtg gccagtgccc atggccggct ggatctgctg gtcaacaatg
    10561 ccggcggctc gccccaggcc agcgcgctgg cctccggccc gcgctttgcc gacgcgatcc
    10621 tgcggctgaa cctgctggcg ccgctttata tggcgcaggc cgcccatccg cacatgcggg
    10681 ccagcggcgg tggcagcatc gtcaacattg ccagcgtgtc ggccattcgc ccgtcgccgg
    10741 gcacggcggt ctatggcgcg gccaaggccg ggctggtcag cctgacgcag agcctggcgc
    10801 aggaatgggg ccccgatggc atccgtgtca acgcgatcat cgccgggctg atggccaccg
    10861 aaaccgccgg cgagacctat ggcgatgcca cagctcaggc ggcggtggcg gcgtccatgc
    10921 cgctgggccg catgggcacg ggcgaggata tcgccggcgc ggtgctgtgg ctggcctcgg
    10981 gcctgtcggg ctgggtcagc ggggcgcgca tcaatgtcga tgggggcggc gagcgcccct
    11041 attttctcga tcttgtgaag ggaggctgag cccatggggg tgaaggcact gggctatgtg
    11101 gtgattgcca ccgcccgttt ggaggaatgg gaccgtttcc tgaccgaagt ggtgggggtg
    11161 atgcgcggcg ggccggcggc cgatggcggc gcgctctacc ggatcgacgg ccggccgttc
    11221 cgtttctggc tgcagccggc cgcgcaagag gcgctggcgg catcgggcta tgagatcgcc
    11281 gatgccgagg gctgggcggc gctgcgcgcg cgcattgtgg ccgccgggcg cccggtgg
//
LOCUS       DASRYE010000310        31221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_47803,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000310 DASRYE010000000
VERSION     DASRYE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 31221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31221
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47803"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFF98_15870"
     CDS             complement(<1..828)
                     /locus_tag="VFF98_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HZU65165.1"
                     /translation="MSSRPAALTPPPDTGQARVAELEARVAQLETINAALIERVERAS
                     DFESGAFSMFETAISLERLVRDRTAALKDALGRLSSANAELAEAHHRAEAARTRLRDA
                     IESLSDGFALFDGTDRLIMCNDAYRRFWPEIADLSENAPFRDVVLAVARNGRTMAALR
                     SPERWVADRVAEHATADGVHVHALADGRWLQINEIRTSEGGTVGIYTDITEVKAEDAR
                     ERARELAEHNLALQATLETLSEGVCLYDAHQRLLVYNGGLARILRLPAGDRRAIATHA
                     "
     gene            complement(825..1976)
                     /locus_tag="VFF98_15875"
     CDS             complement(825..1976)
                     /locus_tag="VFF98_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZU65166.1"
                     /translation="MAQQAARADLDLVVASSHAAEPAAAAAELAAAMGSRPLAGGLVF
                     CSHRLAGQALAEALQQVLGDMPLIGCTSAGELSDRGYDADSVVFIGFPADAFRMAVHC
                     FDDLDHFVPAEGRQAITALAAVAAGGEEGGAFPNRAALFLVDGLSHREELLAMTVQEA
                     LGDTVMVGGSSGDGLRFRETGILVGGRFVKDAAAVAILASRRPMQGFTAHHYRPGAEK
                     MVVTAADPHARTVFQINARPAAEEYRRVAGRLGEPLDPAFFAAHPPMVRAGGGYHVRA
                     VQSVNADDSLTFYCAIDNGIVLTIGEPVDRLAQLEALFDDLEGEIGQIDHVIGFDCVL
                     NRIDAENRQLTGAVSRFYARRRVVGFNTYGEQYRAAHINQTLSGLVIGR"
     gene            complement(2098..2502)
                     /locus_tag="VFF98_15880"
     CDS             complement(2098..2502)
                     /locus_tag="VFF98_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HZU65167.1"
                     /translation="MAIPPRVLATPAAQALIARLAALHGPLMFHQSGGCCDGSAPICM
                     PRGTLRLGGQDVLLGHVAGDTPVWIGAAHFDYMRHTRMTIDAAPGRGAGMSLETPEGM
                     RFLVHSELFSEEEAQSLAAAGEPLRGDSLPPA"
     gene            complement(2637..4157)
                     /gene="adh"
                     /locus_tag="VFF98_15885"
     CDS             complement(2637..4157)
                     /gene="adh"
                     /locus_tag="VFF98_15885"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU65168.1"
                     /translation="MLHEALGKFAGKSVIREKYDNFIGGKWVAPVQGRYFDNISPLTG
                     APVCQVARGTAEDIELALDAAHAAREGWARRSPTERSNILLKIADRLEANLDLLALVE
                     TIDNGKPIRETTLADIPLAIDHFRYFAACVRAQEGSIAEIDHDTVAYHFHEPLGVVGQ
                     IIPWNFPILMAAWKLAPAIAAGNCVVLKPAEQTPMSILVLAEVIADILPEGVLNVVNG
                     FGIEAGKPLATSKRIAKIAFTGETSTGRLIMQYASENLIPVTLELGGKSPNIFFADVM
                     AEDDDFLDKALEGFSMFALNQGEVCTCPSRALVHESIYDRFIEKAVKRVEAIKLGSPL
                     DLSTMIGAQASNEQMEKILSYIEIGKGEGANVLTGGARHLHEGELAGGYYVKPTVLEG
                     HNKMRIFQEEIFGPVLSVTKFSSDEEALHIANDTLYGLGAGVWTRDGTRAYRFGRAIQ
                     AGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNLLVSYSPKKLGFF"
     gene            complement(4301..5821)
                     /locus_tag="VFF98_15890"
     CDS             complement(4301..5821)
                     /locus_tag="VFF98_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU65169.1"
                     /translation="MEELVKLRQQGLRVLVGIAWLSCLIVAGGSFFAGSGVLPVVLAV
                     ALGIYPTLAAMGGQCDEGTRIVMGGTMPLYCAVLLYQWAGHGWIIDLHMTFFAAIAVL
                     VVLADWRALVAGAGVTAVHHLLLNFVAPALVFAGGGDLGRVVLHAVVVVVETAVLVAM
                     AMRLEQLLLAQVAAREAKERLEAAAAAERARREEEQETVVAAIALGLGRLAQGDLASR
                     IEARFPAGFEPLRENYNRAIGDLDRLVGSVARASGQIQNGVREIRYASDDLARRTEQQ
                     AASVERATHTIGELVHAASETAARAAETSGTLDQSQQQAARGHAVVAEAMATMERIEQ
                     SAGEIGQIVSLIDGIAFQTNLLALNAGVEAARAGDSGRGFAVVAGEVRALAQRSAEAA
                     ADIKRLIGNSTAQVSEGVALVTQTGQALQEVMEQVTMIACTVSEITAAVKHNACELGD
                     VREAFSGLDRSTQQNAAMVEESNAALQTLVNETNGLIGSIDRFKGSARQGAARLAA"
     gene            complement(5901..6194)
                     /locus_tag="VFF98_15895"
     CDS             complement(5901..6194)
                     /locus_tag="VFF98_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65170.1"
                     /translation="MPAPRAWRGQVVVSVLVLGLCLFAPRAGAGLLLLPCWPTVAAPG
                     APFLARHGLALIGAGRLPGSLVVHAAGPVPVGALLARGLVPLALPAALCGGRP"
     gene            complement(6317..7243)
                     /locus_tag="VFF98_15900"
     CDS             complement(6317..7243)
                     /locus_tag="VFF98_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU65171.1"
                     /translation="MSLPDLEAWAIFAKVVDTGSFAAAAEVLGLSKPTVSKAITRLEG
                     RLGVPLLHRTSRRLALTESGRGVLEKARRILADGEAAEAQASAESAAPQGLVRLAVPM
                     SFGLRHVAPILPEFLDTFPAVRVEVDLSDALIDVVGGGHDVVLRIAELGDSSLRARRL
                     CTVRRPLVAAPAYLAAHGTPTHPRELSAHAGLLYSNLEAPGSWRFTHTGEGDYLVQVS
                     GRLAANNADALTPALLAGQGMALQPDFMVWEHLASGALVEVLPDWRLPDIALHLLTPP
                     GALRAARVQVLIDFLARRLAQAEWANMGRRAV"
     gene            7357..8055
                     /locus_tag="VFF98_15905"
     CDS             7357..8055
                     /locus_tag="VFF98_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZU65172.1"
                     /translation="MIELRPFAQIGHANHGWLDARHHFSFADYYDPARMHWGNLRVWN
                     DDTIAAKSGFPPHPHRDMEIITYVRTGAITHKDSLGNQGRTAAGDVQVMSAGTGITHA
                     EYNLEDETTTLFQIWILPTRGGESPGWGARQFPRGNRAGQLVVLASGYAEDADALPIR
                     TEARLVAGTLRAGESVDYPLGADRKAYLVPATGRIAVNGVEAAARDGIAIADEEHLTI
                     TALEDSEVVLVDTL"
     gene            8122..9042
                     /locus_tag="VFF98_15910"
     CDS             8122..9042
                     /locus_tag="VFF98_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZU65173.1"
                     /translation="MTLPASHTPVDAVIVPPVRDLGDGFTVRRALPSPNRRMVGPFIF
                     LDQMGPAAFSGEEALDVRPHPHIGLATVTYLLEGEILHRDSLGSEQPIRPGEVNWMTA
                     GSGIVHSERTPAAQRRSGGVLFGLQTWVALPERQEETAPSFRHYKADAIPVGEADGAR
                     LTLIAGTADGLASPVETFSDMIYADLVLAAGGRYRFGAEHVERAVYVVSGALAIEGQE
                     GTFGPAQLVVLKPGAEVVLAAREPTRLMLVGGEPFAEKRYIYWNFVSSRADRIEQAKA
                     DWRERRFAPVPGDTEEFIPLPADVPGARLK"
     gene            9054..9467
                     /locus_tag="VFF98_15915"
     CDS             9054..9467
                     /locus_tag="VFF98_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZU65174.1"
                     /translation="MAHIATASAASTGAATFQSRTDIAGTAITTDEPVELGGLGSGPN
                     PYDLLCAALATCTTMTLRFYAARQGWEIGEVATHVTHRREAGASPADVFNRTITLAAS
                     LPADQRTRLLDVAERCPVHKTLSAGAAIHTRGDNA"
     gene            9464..9778
                     /locus_tag="VFF98_15920"
     CDS             9464..9778
                     /locus_tag="VFF98_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65175.1"
                     /translation="MNAAAPALPRDIALAAFIGAQDAARCGCREPAAPREELGWSSRL
                     LLWLLGDNRPRPLANPRLEAVRRFACATRAGHKPGIALVSELHNLGLQPAHLALLSEF
                     ML"
     gene            complement(9858..12047)
                     /gene="katG"
                     /locus_tag="VFF98_15925"
     CDS             complement(9858..12047)
                     /gene="katG"
                     /locus_tag="VFF98_15925"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HZU65176.1"
                     /translation="MSEVGKCPFHADKVSRAAIVRALDNQMWWPERLDLKILNKNAPQ
                     IDPLGGVFDYPAAFAALDLKAVKADLFALMTDSQDWWPADFGHYGPLFIRMAWHSAGT
                     YRISDGRGGAGAGQQRFAPLNSWPDNVNLDKARLLLWPVKQKYGAALSWADLMILAGN
                     CALESMGLQTAGFSGGRADTWEPEDDVYWGPEATWLADERYTGDRDLEDPLAAVQMGL
                     IYVNPEGPDGNPDPLASARDIRETFARMAMDDYETVALIAGGHTFGKTHGAGDAALVG
                     PEPEAAPIEQLGLGWKSAYGSGKGGDAIGSGLEVIWSETPTQWSNNFFKNLFGYEWEL
                     TKSPAGAHQWTPKGGAGAGTVPDAHDPARRHAPSMLTSDLALRFDPAYEKISRHFYKH
                     PDEFARAFAEAWYKLTHRDMGPRARHIGPEVPAEPRIWQDPVPAVAHPLISEEEAAVL
                     KGEILASGLSNARLVKTAWAAASTFRGSDKRGGVNGARIALAPQKDWAVNEPDELADV
                     LQVLKGIQAAFNAKGASQVSLADLIVLAGNAAVEEAALAAGYEVLVPFHPGRTDATDA
                     MTDAASFAVLEPRVDGFRNYQGEASGRSPEEWLVDRANLLTLTAPEMTALLGGLRVLG
                     GNVEADRRGVFTERVGVLTNDFFVNLLASATSLTWSEEEGNYVGRDRASGGEVYTASR
                     VDLIFASHSQLRAISEVYAAADGEARFIAAFVQVWHKVMELDRFDLK"
     gene            complement(12254..12967)
                     /locus_tag="VFF98_15930"
     CDS             complement(12254..12967)
                     /locus_tag="VFF98_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU65177.1"
                     /translation="MNLPLARSPRSPKPGQRVMVALRHMIVTGEIADGARIAEIPTAE
                     RLGVSRMPVRTALRALALEGLVVPLGKRGYAARRPDQAMKEGALQVRGVLEGLAARLV
                     AQAPDHAATGARLAAIIAPCAGIGAQGRLAEGALALYAEANAGFHAALLAACTNGAIG
                     MALAQNDHIPLASAVAFAFDTQDEASVAGLLAAAHAQHAAVARAIAEGAAEAAEALMR
                     SHAEASLAMERGLAAEPVA"
     gene            13042..14082
                     /locus_tag="VFF98_15935"
     CDS             13042..14082
                     /locus_tag="VFF98_15935"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU65178.1"
                     /translation="MPNTPWPVNAWYVAARAEELGDAPLGRQICGERMVFFRGIDGTV
                     AALEDFCPHRGAPLSLGFVREGALVCGYHGLALACSGKVAGMPGQKLAGFPPVRRFAV
                     EERHGFLWVWPGAAEAADPALIPALVWADSPQWAYGGGYYHIACDYRLMIDNLMDLTH
                     ETYVHASSIGQKEIDEAPVKTRREGEEILTSRFMEGVIAPPFWQMALRAAGLPAEAPV
                     DRWQICRFSLPSHVMIEVGVAMAGEGGIAAPDARKVYSVVVDFITPETATSHHYFWGM
                     ARRFGVEDAALTEAIRAGQGRIFGEDLEMLERQQANLQRWPDRRLLLLNIDAGGARSR
                     QMIEGAIAAETA"
     gene            14378..15694
                     /locus_tag="VFF98_15940"
     CDS             14378..15694
                     /locus_tag="VFF98_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZU65179.1"
                     /translation="MRYKISLMAAAAAAPLLMAAPAQADSKPAFGAPIALGDGFSLDP
                     IVEGRLRLEDVQQPQKALAADAVTMRLRAGFELKNAPSHLALLAEAEGNLALDGAYNA
                     FPYTLKTSSQYRPTHAVVADPDSVGLNRLQLQFKTKPLTLTLGRQRINLDDQRWVGAS
                     GWRQNEQTFDAVRGQVALGPVSFDGSFAESQRSIYGTDGGPRVAYDGRFAFLNAGIGN
                     QIATLKGFAYLLDYNNSAFQNLKATRGQLDSSQTYGLRTTAKLPIEKGVSLALAGSYA
                     RQYSYAANPRHYAADYYAVEGNLAACGFTLTGGYEMMGADAHAVGGPWSVQTPMATLH
                     AFDGWADVFLTTPARGLGDTYAGLAYAFPKAVPVPGLNAKVIYHQFDSDIGNVNYGHE
                     WDAAIGFKTGFLNWLVKYADYRAVGASALGAGTTTRKFWLQSEFAF"
     gene            complement(15730..17058)
                     /locus_tag="VFF98_15945"
     CDS             complement(15730..17058)
                     /locus_tag="VFF98_15945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU65180.1"
                     /translation="MASDRQLHLGAFMRPISIHTGAWRYPGAAPDGNFNFPLIRRLAQ
                     RLEAARFDAFFMADHLAVLNMPASALKRSHTVTSFEPFTLLSALATATERIGLVATAS
                     TTYDEPYHIARRFASLDHLSEGRAGWNIVTTGNPETAHNFGRDEHLEHAARYARAREF
                     HDVVTGLWDSWADDAFVRDAESGVYFDPDRLHVLNHEGPFLKVRGPLNIARPVQGWPV
                     IVQAGASEAGRQLAAETAEVIFGAAETLARGQAFYADVKGRMAALGRAADSLKILPAA
                     FVVVGDSEEEAQAKRARLDSLVHYDSGIRSLSIALDYDVSGFDPDGPLPPIPESNASK
                     SGRDRFIEAGARPGVTIRQLAARAGAYAGLAFVGTAATIADRMEEWLVEKGSDGFNVM
                     FSHAPGGVEDFCDKVVPELQRRGLFRREYAGTTLRDHLGLARPANRFFPA"
     gene            complement(17162..19459)
                     /locus_tag="VFF98_15950"
     CDS             complement(17162..19459)
                     /locus_tag="VFF98_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65181.1"
                     /translation="MSRRSPTRALLLASALLLPAAPALADEAAPAAPVPAAPAADQAN
                     GDIVVTARYRNESLSHVPIAITAVSGASLNDRALHNLQDLAAAVPTIDFRNGASNKDR
                     TVFIRGIGTISTSPGVEPSVSTVVDGVVLTRPGQATLDVGEVDRVEILRGPQGTLFGK
                     NASAGVVSVVTAAPSERLGGYAETGITTDTEYRLKAGITGPLAENLTGRIDGLYTHYN
                     GNVKNVTTGNEVNGYDRTGLRAKLQYTPAPNVKLTLAGDYLRSHDTVPNGVFASASQS
                     AYPTGAVSTSSALASYFAGQGVALGADNRTVTSSYDSDVRDRNYGTSLTGEWRLNNGY
                     TLTSITAYRGWTNHQHQDYDQIGQLTSSLYQGSDRGDLSAHQFSQELRLTSPKGRFID
                     YVVGAYYLYAQDDERYQRDVTKLISGTATPYEGIASYGTRSNNYALFGEANVNFTSRL
                     RAILGYRSSWDKLAYYHQRTSTNDPTNSGASALDVTGIGAYHASAGSTDARGDTARAG
                     LQYDFARGNQIYFTWSRGYKGPAFDAFFNMRARDEITLAPETSNAFEVGAKGSTLGHQ
                     ITYAVDVFSTVINNYQANFNDVVGGSPVTRLVNAGRVGTRGVEIDLGFHPIPGLSFDT
                     SLAYDDAKVLHFNCPAGTPNSCNVDGQPLPFAPKWKLYESASYRFGVARGADLELQTD
                     VSLKSATQYQLTETANTIQPAYAIWNISAALIGHNGWQLRAYIKNLTNTHYSDYLANG
                     NIGGTVRFVPRDDARYAGFTLRKDF"
     gene            complement(19532..20782)
                     /locus_tag="VFF98_15955"
     CDS             complement(19532..20782)
                     /locus_tag="VFF98_15955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65182.1"
                     /translation="MPDALPHPLPDALSPRRRNLLFALALTAGILNLVDRQIIAVLKP
                     VISADLHWNDDDYGTLAALFQAGAAFGFLAAGWLVDRLGVKWANPLGVAAWSVAAMAH
                     GWVRSMAGFAAVRVALGTTEAMGTPSGIKTIATIFPANQRSAGFGMANAMNSAGAVLA
                     PLAIPFVAAAWGWRATFVLSGAVGLVWAGLWLLATRGLALRGAASVQAAGGSLLRQRQ
                     TWTIAGAKVLSDATWWLLLFWMPDYFHRQFGLGGTGLALPLALAYGGAAVGSLASGFV
                     AARLLERGVSVNAVRKGVMLASALCVVPVPLALGSQAIWAAVAIMALALAGHQGFSTS
                     LFGLIADVVPRNAVGRVTGFGSFCGNLGGIAIAKISGLVLAAGLGYAPLFGFACLSYL
                     LALGWIQLMLGRICPVAEGGSVGG"
     gene            complement(20879..21934)
                     /locus_tag="VFF98_15960"
     CDS             complement(20879..21934)
                     /locus_tag="VFF98_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZU65183.1"
                     /translation="MTTPVEISWFSALCDDDYEFLGQNEPALQSSWEHCRNIVLAAEE
                     AGFDNILLPNGYQLGIDTAAFAAAIATQVKRIKLLMAVRVGELWPPQLARQIATINQI
                     LGGRLNVNIISSEMPGEVLDGPARYERTLEAITILRALLAGEGLDHQGRFWNLKLDPA
                     RILTGTACPPLYFGGLSEPAREVAAKGCDVYLMWPDRLENVRAVVADMKARAAREGRQ
                     LKYGYRVHVVVRETEAEARAAADRLLSKLDAATGAAIKAKSLDTQSVGVAAQVALREA
                     AAGDGYVEDHLWTGIGRARSGCGAAIVGDPDQVLAKLQAYRQAGIDAFVLSGYPHARE
                     ADLFARYVLPRIDHAPF"
     gene            complement(21931..22863)
                     /locus_tag="VFF98_15965"
     CDS             complement(21931..22863)
                     /locus_tag="VFF98_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU65184.1"
                     /translation="MTDTRSDQNSTICVETHPVPLGKSGLSVFPIAWGMWRIASPAGQ
                     DDIGAIIARIEAALEAGITLFDTADIYGAGEPIGFGGAEALLGQALARRPDLRARMIL
                     ATKGGIWPGVPYNSTTAYLAGAIDASLARLGVDSVELYQIHRRDFLAHPREVAEALAA
                     MVAAGKVKHVGVSNYSPAEVAALQAFLPLPLVSTQPEFSPAHYAPLYDGTLDDAMARE
                     MAVLAWSPLGGGRLARGDHPAAALLAAQGAKYEADAAAAALSWIMAHPAKIVPIVGTQ
                     TPERILDSGDAFRIRWTRNEWYAVLEASRGERMP"
     gene            22893..23975
                     /locus_tag="VFF98_15970"
     CDS             22893..23975
                     /locus_tag="VFF98_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZU65185.1"
                     /translation="MVETIRYGIIGTGMMGVEHIHNLQILPGAEIVAVADPDATSLGW
                     ARKAIGHEVAEFADAAALVKGADVDAVVVVSPNFTHRAVLEPLFDADVAILCEKPLCT
                     TMADARWVAAKARAHHRPFWTGMEYRFMPPAAEFIAQVHGGRVGALQMLSIREHRFPF
                     LVKVGDWNRFARNTGGTMVEKCCHFFDLMRLIVRSEPVRVYCSGAMDVNHLDERYDGQ
                     RPDIIDNSFTTIDFANGVRAMLDLCMFAEGAEHQEEISATGDRARLDVLIPPGDLVYA
                     PRVDWRGPKAVERTHVAVDEAALKAGSHHGSTYYQHEAFARAVRGEGPVIVSAEDGLM
                     AVAIGTAAEISAREHRVVDMAEVLEA"
     gene            complement(24006..25379)
                     /locus_tag="VFF98_15975"
     CDS             complement(24006..25379)
                     /locus_tag="VFF98_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZU65186.1"
                     /translation="MPASPSPANGADLPIAIVGAGFSGTLLAINLLRLGARVVLVERD
                     REKLARGLAFGTRAPEHLLNVRAANMSAFPDDPGHFLRWMGFSSQEQANRFAPRLTYG
                     QYLREQLLAALAAAPGRFAIREEEALGIDWHADHAALRLASGQVIAARAVVLALGNFA
                     PARLPVLAGLAPPVLIDNPWSAAVTQDLEGIDHVLLVGTGLTAVDVALSLEKAGYRGR
                     ITALSRRGLAPRAHAAEGPVVGAVPRPEARGSWLLRQLRRRAGHVGWRHAVDELRAHT
                     QSLWRQHDAAAQARFLRHLRPWWDVHRHRLAPPVAEVIDRLRADGRLDFVAGRLLGAE
                     PRGDGAHVRWQPRGGGPERQLDVGRVVLCTGPEGDITRSDHPLLRALVAEGRARADVH
                     RLGLDVDHTGSVLDAQGRRQPHLYAVGPVTKGEAWEIIAVPDIRRQVWHLARMLSNTH
                     WIGGEGL"
     gene            25657..26499
                     /locus_tag="VFF98_15980"
     CDS             25657..26499
                     /locus_tag="VFF98_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZU65187.1"
                     /translation="MGFIGHLDLLHALAGLLVGLLVGLTGVGGGSLMTPLLVLLFGVA
                     PQTAVGTDLLYAAITKITGSAVHGWRDTVEWSIVRRLAYGSVPAAIATLAVLAHIGHP
                     DPATQNIILLVLAVLLALTALSAFFRNWLIRFAADLDPLDPPFKAVVGTVVLGAVIGV
                     AVSVSSVGAGAIGVTVLLMLYPRLPVVRVVGSDIAHAVPLALIAGVGHWIIGDVDGAL
                     LTNLLVGSIPGVVIGSLLSTRAPDKVLRPLLAVVLAISSWQLFLKTLSHEHTAPRPAA
                     SPTR"
     gene            26541..27809
                     /locus_tag="VFF98_15985"
     CDS             26541..27809
                     /locus_tag="VFF98_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HZU65188.1"
                     /translation="MPMTMPPRRLAPAAGLLLALASPPAHATDGYFLNGVGAKAEGEA
                     GVAIALPQDALAIAANPAAATEVGHRLDIGAQIFVPDRSATISGNGAGLNGRYGGNGA
                     NPFLLPDIGYVRPLGERLAIGIAIYGNGGMNTTYKANPFASFGASGPAGVDLKQIFIT
                     PTLAARVLPGQSLGISPIIVVQSFKASGLQPFSAYSEDPAHFTNLGESWSTGAGVRVG
                     WQGHFGPALTLGAFYESKVRASHFGKYAGLFAGQGRFDVPAAWGAGIAVKANARLTLA
                     ADVKRIEYAGVGAVGNGLGALFLGVPFGASGGPGFGWRNITSAKFGISYAASPRLILR
                     TGYGWTGNPVPASQTLLNILAPGVVQNHLTLGATWTTRGGVELTGFAMHALGHTVYGN
                     GSIPAAFGGGESDVALGETAFGLSGGWKFR"
     gene            27800..28237
                     /locus_tag="VFF98_15990"
     CDS             27800..28237
                     /locus_tag="VFF98_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZU65189.1"
                     /translation="MPVSLALAGHLPNLAGGALIGLAAGALRLTSGHIAGISGIARLA
                     LRGPERGWRLAFLAGLILAGLIPAGPAASLTAGLAETPLPTLAGAGLLVGLGTGLGNG
                     CTSGHGICGLARLSRRSLVAVAVFMATAMLTVLIRHHTAGIWQ"
     gene            28234..28674
                     /locus_tag="VFF98_15995"
     CDS             28234..28674
                     /locus_tag="VFF98_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZU65190.1"
                     /translation="MSPASARGSTSTLAALGCGLLFGAGLLLSGMTSPARVLGFLDIT
                     GQITGQWDPSLAFVMGGALAVAAPLFALARRRGAALNGRPLADPASRLPDARLLAGAA
                     LFGLGWGLAGLCPGPALVDLALRPWPALVFTGPMLAGLLLSRRD"
     gene            29056..29196
                     /locus_tag="VFF98_16000"
     CDS             29056..29196
                     /locus_tag="VFF98_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65191.1"
                     /translation="MASITKRTVDAAQPGTRDSFLWDDEIAGFGLKITPAGGRPTFIN
                     IG"
     gene            29193..30392
                     /locus_tag="VFF98_16005"
     CDS             29193..30392
                     /locus_tag="VFF98_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZU65192.1"
                     /translation="MTRPGMAKACPTRRHTIGRHGNLTPLQARARAKELAAMVAQGID
                     PGQLEKDRFTAADNARRAAEEKARAESLLAFDRLAETWLDHYENEKERRPSGVALAKL
                     VVNRYLVPALGNRPMPHIGRSDLNPILDSIPTAKRGMRRAVFAYGSILFGWAHKRGDI
                     AENPFLSMVKPEAPKARDRVLNDDELAAIWKATATLSEPFGPFFRLLVLTGQRHREVS
                     DISWTELDPGGALWTIPAGRAKNGAAHLVPLSPAAIAELDALALAQHIKAKAKKPDGA
                     KWPIVGFALTTTGHSPISGISKAKAALDKAITEARKDQGGAIPAWRIHDLRRSLATGF
                     QRLGVRFEVTEATLNHISGAKGGVAGIYQRHDWAEEKRAALDAWARHVAAIRQPADQA
                     NVVKLRA"
     gene            complement(30908..>31221)
                     /locus_tag="VFF98_16010"
     CDS             complement(30908..>31221)
                     /locus_tag="VFF98_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65193.1"
                     /translation="PTPMLTALDGKLNAAWPPCDGTIRFAAYRVYETALDTARLGLAH
                     RDPGIKAPRRRGVTPADITAARSELTALYAAHLGKSPAEAAAMAGDAINSIMATAQEC
                     E"
BASE COUNT         5166 a        11060 c        10077 g         4918 t
ORIGIN      
        1 cgcatgggtg gcaatcgccc gccggtcgcc cgccggcagc cgcaagatcc gcgccagccc
       61 gccattatag accagcagcc gctgatgcgc atcatagagg cacacccctt ccgacagcgt
      121 ttccagcgtg gcctgcagcg ccagattgtg ctcggccagc tcgcgcgccc gctcgcgcgc
      181 gtcctcggcc ttcacctcgg tgatatcggt gtagatcccc accgtgccgc cttcggaagt
      241 gcggatctcg ttaatctgca gccagcggcc atcggccagc gcatgcacat gcacgccatc
      301 ggcggtggca tgttcggcca cccggtcggc cacccagcgc tctggcgagc gcagcgcggc
      361 catcgtgcgg ccattgcgcg ccacggccag caccacatcg cgaaacggcg cattctccga
      421 caggtcggca atttccggcc agaagcggcg ataggcatcg ttgcacatga tcagccggtc
      481 ggtgccatcg aacagggcaa aaccgtcgga cagggattcg atcgcatcgc gcaggcgcgt
      541 gcgggccgcc tctgcccggt ggtgcgcctc ggccagctcg gcattggccg aggacagccg
      601 ccccagcgca tccttcagcg ccgccgtgcg atcgcgcacc agccgctcca gcgagatcgc
      661 cgtctcgaac atggaaaagg cgccgctctc gaaatcgctg gcccgctcca cccgctcgat
      721 cagcgcggcg ttgatggtct ccagctgggc cacccgcgct tccagctcgg ccacgcgcgc
      781 ctggcccgtg tctggcggtg gggtcagggc cgccggccgg ctgctcatcg gccgatcacc
      841 agtccgctca gcgtctggtt gatatgggcg gcgcgatatt gctcgccata ggtgttgaag
      901 cccaccaccc gcctccgcgc atagaagcgc gagacggcgc ccgtcagctg gcggttttcc
      961 gcatcgatcc ggttgagcac gcaatcaaag ccgatcacat ggtcgatctg gccgatctcg
     1021 ccttccagat cgtcgaacag cgcttccagc tgggccagcc ggtccaccgg ctcgccaatg
     1081 gtaagcacga tgccattgtc gatcgcgcaa tagaaggtca ggctgtcatc cgcattgacc
     1141 gactgcaccg cgcgcacatg atagccgccc cctgcccgca ccatcggcgg atgggcggca
     1201 aaaaaggccg gatcgagcgg ttcacccagc cggccggcca cgcggcgata ttcctcggcc
     1261 gccggccgcg cgttgatctg gaacacggtg cgggcatggg gatcggccgc ggtgacgacc
     1321 atcttctcgg cgccggggcg atagtgatgg gcggtgaaac cctgcatcgg ccgccggctg
     1381 gccagaatgg ccacggccgc tgcatccttc acaaagcgcc cgcccaccag aatgccggtt
     1441 tcccggaacc gcaggccatc gcccgaagag ccgcccacca tcaccgtatc gcccagcgct
     1501 tcctgcacgg tcatcgccag cagttcctcg cggtgcgaca ggccatcgac caggaacagc
     1561 gccgcccggt tgggaaacgc gcccccctcc tcgccgccag ccgccacggc cgccagcgcg
     1621 gtgatcgcct ggcgcccctc ggccggcacg aaatgatcga gatcgtcgaa gcaatgcacc
     1681 gccatgcgga aagcatctgc cggaaaacca atgaacacca cgctatcggc atcatagccg
     1741 cgatcactca gttcgccggc gctggtgcag ccgatcagcg gcatatcgcc cagcacctgc
     1801 tgcagcgctt cggccaaagc ctggcccgcc agccggtgcg aacagaacac cagcccgcca
     1861 gccagcgggc ggctgcccat cgccgcggcc agttcggcag ccgctgctgc cggctctgcc
     1921 gcgtgcgaac tggccaccac cagatccaga tcagccctcg cggcctgttg cgccactcct
     1981 ctcctccacc tttggccggg tttgccccgt gtaaggccgg tgttctagcg cagcttgccc
     2041 accgctccaa gcggcaagca cacaaacgcg cgccctacat gccgatttcg cgccgcatca
     2101 ggccggcggc aggctgtcgc cgcgcaaggg ttcgccagcg gcggccaggc tttgcgcctc
     2161 ttcctcgctg aacagttcgc tatgcacgag aaaacgcatg ccctcgggcg tttccaggct
     2221 catgccggcg ccgcgcccgg gggcggcatc gatggtcatc cgggtgtggc gcatatagtc
     2281 gaaatgggca gcgccgatcc acaccggcgt atcacccgcc acatggccca acagcacatc
     2341 ctgcccgccc agccgcaagg tgccgcgcgg catgcagatc ggcgccgaac catcgcagca
     2401 gccgccagac tggtggaaca tcaaaggccc atgcagcgcc gccagccggg cgatcagcgc
     2461 ctgggcggca ggggtggcca acacgcgcgg gggaatggcc atgggcacct gcgggcaaat
     2521 ggaggcaaga aagccgcccg tttcgggaga ggacgcgaac ctgaaggggc ggctttcccc
     2581 cttcgccctt ttgcggccgg ggccagcatg ccgccccagc cgcaaaaggc cgtttctcag
     2641 aagaagccca gcttcttggg gctgtagctc accagcaggt tcttggtctg ctgatagtgg
     2701 tcgagcatca tcttgtgggt ttcgcggcca atgcccgact gcttgtagcc accgaaggcc
     2761 gcatgggccg gataggcgtg atagcagttg gtccacacgc ggccggcctg gatggcgcgg
     2821 ccaaagcgat aggcgcgggt gccatcgcgg gtccacacgc cggcgccaag gccatagagc
     2881 gtgtcgttgg cgatgtgcag cgcttcctca tccgacgaga acttggtgac cgacagcacc
     2941 gggccaaaga tctcttcctg gaagatgcgc atcttgttgt ggccttccag cacggtcggc
     3001 ttcacgtaat agccgccggc cagctcgccc tcatgcaggt ggcgggcgcc gcccgtcagc
     3061 acattggcgc cttcgccctt gccgatctcg atataggaga ggatcttctc catctgctcg
     3121 ttcgaggcct gggcgccgat catcgtcgac agatccagcg ggctgcccag cttgatcgcc
     3181 tccacgcgct tcaccgcctt ctcgatgaag cggtcgtaga tcgattcatg caccagcgcg
     3241 cggctcgggc aggtgcacac ctcgccctgg ttcagcgcga acatgctgaa gccttccagc
     3301 gccttgtcga ggaaatcatc gtcctcggcc atcacatcgg caaagaagat gttggggctc
     3361 ttgccgccca gttccagcgt caccgggatg aggttttcgc tggcatattg catgatcagg
     3421 cggccggtgc tcgtctcgcc ggtgaaggcg atcttggcga tgcgcttgct ggtggccagc
     3481 ggcttgccgg cctcgatgcc aaagccgttc accacattga gcacgccctc gggcaggatg
     3541 tcggcgatca cctcggccag caccaggatc gacatcggcg tctgctcggc cggcttcagc
     3601 accacgcaat tgcccgcggc aatggccggc gccagcttcc aggcggccat caggatcggg
     3661 aagttccagg ggatgatctg gcccaccacg cccagcggct cgtggaagtg ataggcgacc
     3721 gtgtcatggt cgatctcggc gatcgagcct tcctgggcgc gcacgcaggc ggcaaaatag
     3781 cggaaatgat cgatggcgag gggaatgtcg gccagcgtcg tctcgcggat cggcttgccg
     3841 ttgtcgatgg tttccaccag cgccagcagg tcgagattgg cctccagccg gtcggcgatc
     3901 ttcagcagga tgttcgaacg ctcggtgggc gaacggcgcg cccagccctc gcgggcggca
     3961 tgggcggcat cgagggccag ctcgatatcc tcggccgtgc cgcgcgccac ctggcacacc
     4021 ggggcgccgg tcagcggcga gatattgtcg aaatagcggc cctgcaccgg ggcgacccac
     4081 ttgccgccga tgaaattgtc gtatttttcg cgaataacgg atttgccggc aaacttgcca
     4141 agggcctcgt gcagcatctc aatccctcct gggtgtgtgg ccttccgcaa ctggcgcgcg
     4201 ggtgggcaat ttcgccgcca aacctgctcg cccgcgcctg ccctgccaat tataccttag
     4261 gccgagaggg attttccgcc cgcatccgcg cgcctcgggc tcaagccgcc agccgcgccg
     4321 cgccctgccg ggcagagccc ttgaagcggt cgatcgagcc gatcagcccg ttggtttcat
     4381 tgaccagcgt ctgcagcgcg gcgttgcttt cctccaccat cgcggcattc tgctgggtgg
     4441 aacgatccag ccccgagaaa gcctcgcgca catcgcccag ttcgcaggca ttgtgcttca
     4501 ccgccgcggt gatctccgag acggtgcagg cgatcatcgt cacttgctcc atcacttcct
     4561 gcagcgcctg gccggtctgc gtcaccagcg ccacgccctc gctcacctgg gcggtggaat
     4621 tgccgatcag ccgcttgatg tcggccgccg cctcggcgct gcgctgggcc agcgcgcgca
     4681 cttcgccggc caccacggca aagccgcggc ccgaatcccc cgcgcgcgcc gcttccacgc
     4741 cggcgttcag cgccagaagg ttggtctgga aggcaatgcc atcgatcagc gagacgatct
     4801 ggccaatctc gccggccgat tgctcgatcc gctccatcgt tgccatcgct tctgccacca
     4861 ccgcatggcc gcgcgcggcc tgctgctgcg actggtcgag cgtgccgctg gtttcggccg
     4921 cgcgggcggc ggtttcgctg gcggcatgca ccagctcgcc aatcgtgtgg gtggcccgct
     4981 ccaccgaggc ggcctgctgt tcggtgcggc gcgccaggtc atcggaggca tagcggatct
     5041 cgcgcacgcc gttctggatc tggccggagg cacgcgccac gctgcccacc agccggtcga
     5101 gatcgccgat cgcgcggttg taattctcgc gcagcggctc gaagccagcg gggaagcggg
     5161 cctcgatgcg gctggccaga tcgccctgcg ccagccggcc cagccccagc gcgatcgccg
     5221 ccaccaccgt ttcctgctct tcctcgcggc gggcgcgttc ggcagcggcg gccgcttcca
     5281 gccgttcctt cgcctcgcgc gcggccacct gcgccagcag cagctgttcc agccgcatgg
     5341 ccatggccac cagcacggcg gtttccacca ccaccaccac cgcatgcagc accacccggc
     5401 ccagatcgcc cccgcccgca aacaccagcg ccggcgccac gaaattgagc agcagatgat
     5461 gcaccgccgt aacccccgcg ccggccacca gcgcccgcca atcggccagc accaccagca
     5521 ccgcaatggc ggcaaagaag gtcatgtgca gatcgatgat ccagccatgg ccggcccact
     5581 gatacagcag caccgcgcaa tacagcggca tggtgccccc catgacgatg cgcgtgccct
     5641 cgtcgcactg gccgcccatg gccgccagcg tgggatagat gcccagcgcc accgccagca
     5701 ccaccggcaa caccccgctg ccggcaaaga agctgccgcc agccacgatc aggcagctca
     5761 gccaggcaat gcccaccagc acgcgcaggc cctgctggcg cagtttcaca agttcttcca
     5821 tggtcccaac cgctttctgc ttatgtgaca acaccggccg caaggccccg gcccgatccg
     5881 cccgatttga tctgtccgct tcatggccgc ccgccgcaca gggccgccgg cagcgccaat
     5941 ggcaccagcc cgcgcgccag caaggcgccc accggcaccg gccctgccgc atgcaccacc
     6001 aggctgcccg gcaggcgccc ggcgccgatc agcgccagcc catgccgcgc aaggaacggc
     6061 gcgccgggcg ctgccaccgt gggccagcag ggcagcaaca gcaaccccgc ccccgcccgc
     6121 ggcgcaaaca ggcacaggcc cagcaccagc acgctcacca ccacctgccc gcgccaggcg
     6181 cggggcgcag gcacagcggc ccggcggggc caagagcggg gcccaaagcg gggagagggc
     6241 ggaaaggcaa acatgccttt tgcctaccag caattgctgt gccttcgcct tgtgccggcc
     6301 taggggtttg cctgtatcaa accgcccgcc ggcccatatt cgcccattcc gcctgcgcca
     6361 gccgccgggc aaggaaatct atcagcacct gcacccgggc cgcgcgcagg gcgcccggcg
     6421 gggtcagcag gtggagggcg atatcgggca ggcgccagtc gggcagcact tccaccagcg
     6481 cgccgctggc cagatgttcc cacaccatga aatcgggctg cagcgccatg ccctgccctg
     6541 ccagcagcgc cggcgtcagc gcatcggcat tgttggcggc cagccggccg ctcacctgca
     6601 ccagatagtc gccctcgccc gtatgggtga agcgccagct gcccggcgcc tccagattgg
     6661 aatagagcag cccggcatgg gccgacagtt cgcgcgggtg ggtgggcgtg ccatgggcgg
     6721 ccagataggc cggcgctgcc accagcggcc ggcgcaccgt gcacagccgc ctggcccgca
     6781 gcgaggaatc gcccagctcg gcaatccgca gcaccacatc atggccgccc cccaccacat
     6841 cgatcagcgc gtccgacagg tccacctcca ctcgcacggc ggggaacgtg tccaggaatt
     6901 ccggcaggat cggcgcgaca tggcgcaggc caaagctcat cggcacggcc agccgcacca
     6961 gcccctgggg cgcggcgctt tcggcgctgg cctgcgcctc ggcggcctcg ccatcggcca
     7021 ggatgcgccg cgccttctcc agcaccccgc gcccgctttc cgtcagcgcc aggcggcggc
     7081 tggtgcggtg cagcagcggc acccccagcc gcccctccag ccgggtgatc gccttggaca
     7141 cggtgggctt ggacagcccc agcacctcgg ccgcggcggc aaaggagccg gtgtccacca
     7201 ccttggcaaa gatcgcccaa gcttccagat cgggcaggct catcatgcaa ttcctttcac
     7261 cgatggtttt ccaccattgc catttctttg caagagcgcc gcccctacat tgccctcaac
     7321 agccgctccc ccaagcggcg cccaaggaga aacccgatga tcgagctgcg ccccttcgcc
     7381 caaatcggcc atgccaacca tggctggctg gatgcccgcc accacttctc ctttgccgat
     7441 tactacgatc cggcccgcat gcactggggc aacctgcgcg tgtggaacga cgacaccatc
     7501 gcggccaagt cgggcttccc cccccacccg caccgcgaca tggaaatcat cacctatgtg
     7561 cgcaccggcg ccatcaccca caaggacagc ctgggcaacc agggccgcac cgccgccggc
     7621 gacgtgcagg tgatgagcgc gggcaccggc atcacccatg ccgaatacaa cctcgaggac
     7681 gagacgacga cgctgttcca gatctggatc ctgcccaccc gtggcggtga aagccccggc
     7741 tggggcgccc gccagttccc ccgcgggaat cgcgccgggc agctggtggt gctggcctcg
     7801 ggctatgccg aggatgcaga tgccctgccg atccgcaccg aggcgcggct ggtggccggc
     7861 acgctgcgcg ccggcgaaag cgtggattat ccgctgggcg ccgatcgcaa ggcctatctg
     7921 gtgcccgcca ctggccgcat cgcggtcaat ggcgtggaag cggccgcgcg tgatggcatc
     7981 gccattgccg atgaggaaca cctcaccatc accgcgctgg aagacagcga agtggtgctc
     8041 gtcgacaccc tgtaatcccc ccaccgggag ggcccgcccc cttccagccc tcccggcacc
     8101 cccatctgaa aggcccgcat catgaccctt cccgccagcc acacccctgt cgatgccgtg
     8161 atcgtgccgc ccgtgcgcga tctgggcgat ggtttcaccg tgcgccgggc gctgcccagc
     8221 cccaaccggc gcatggtggg ccccttcatc ttcctcgacc agatgggccc tgccgccttc
     8281 tcgggcgaag aggcgctgga tgtgcggccc cacccgcata ttggcctggc caccgttacc
     8341 tatctgctgg aaggcgagat cctccaccgc gacagcctgg gcagcgagca gcccatccgc
     8401 ccgggcgagg tgaactggat gacggcgggc agcggtatcg tccattccga acgcacgccg
     8461 gccgcccagc ggcgcagcgg cggcgtgctg tttggcctgc agacctgggt ggcgctgccc
     8521 gagcgccagg aggaaaccgc cccctccttc cgccactaca aggccgatgc catccccgtg
     8581 ggcgaggccg atggcgcccg cctcacgctg atcgccggca ccgccgacgg gctggcctcg
     8641 ccggtcgaga ccttctccga catgatctat gccgatctcg tgctggctgc cggcgggcgc
     8701 tatcgcttcg gggctgaaca tgtggagcgc gcggtctatg tcgtctcggg cgcgctcgcc
     8761 atcgaaggcc aggaaggcac tttcggcccc gcgcagctgg tggtgctgaa acccggcgcc
     8821 gaagtggtgc tggccgcccg ggaacccacc cggctgatgc tggtgggcgg cgagcccttc
     8881 gccgaaaagc gctacatcta ctggaacttc gtctccagcc gggctgatcg catcgagcag
     8941 gccaaggcag actggcgcga gcgccgcttt gcccccgtgc ccggcgatac ggaagaattc
     9001 atccccctgc cggccgatgt gcccggcgcc aggctgaagt gaggagcccc gccatggccc
     9061 atatcgcaac cgccagcgct gccagcaccg gcgctgccac cttccagagc cgcaccgaca
     9121 tcgccggcac cgccatcacc accgacgagc cggtggaact gggcggcctg ggctctggcc
     9181 ccaaccctta cgacctgctg tgcgccgcct tggccacctg caccaccatg accctgcgct
     9241 tctatgccgc gcgccagggc tgggagattg gcgaggtggc cacccatgtg acccaccggc
     9301 gcgaggccgg cgccagcccg gccgatgtct tcaatcgcac catcaccctg gccgccagcc
     9361 tgcccgccga ccagcgcacc aggctgctgg acgtggccga acgctgcccg gtgcacaaga
     9421 cgctgagcgc cggcgctgcc atccacaccc ggggagacaa cgcatgaacg ccgccgcccc
     9481 cgccctgccg cgcgatatcg cgctggccgc cttcatcggc gcgcaggatg ccgctcgctg
     9541 cggctgccgc gagccggccg ccccgcgtga ggaactgggc tggagcagcc gcctgctgct
     9601 gtggctgctg ggcgacaacc gcccccgccc gctggccaat ccgcggctgg aagcggtgcg
     9661 ccgctttgcc tgcgccaccc gcgccggcca caagccgggc atcgccctgg tgagcgagct
     9721 gcacaatctg ggcctgcagc cggcccatct ggccctgctt tccgaattca tgctctgaca
     9781 ggcaggcggg gggcaggcgg ccctgctgcc ccccgccccc acttgccgat cacttcaggc
     9841 cggtcacttc aggccggtca cttcagatcg aagcgatcca gttccatcac cttgtgccac
     9901 acctgcacga aagcggcgat gaagcgcgcc tcgccatcgg ccgcggcata gacctcgctg
     9961 atcgcccgca attgcgagtg cgaggcgaag atcaggtcca cccggctggc ggtgtagact
    10021 tcccccccgc tggcacgatc gcggcccacg taattgcctt cctcctcgct ccaggtgagg
    10081 ctggtggcag aagccagcag gttgacgaag aagtcgttgg tcagcacgcc cacccgctcg
    10141 gtgaacacgc cgcgccggtc ggcttccaca ttgccgccca gcaccctgag gccgcccagc
    10201 agcgcggtca tctccggcgc tgtcagcgtg aggaggttgg cgcggtccac cagccattcc
    10261 tcggggctgc gcccgctggc ctcgccctga tagttgcgga agccatccac cctgggctcc
    10321 agcacggcaa agctggcggc atcggtcatc gcgtcggtgg catcggtgcg gccggggtgg
    10381 aagggcacca gcacttcata gccggccgcc agcgccgctt cctccaccgc cgcattgccg
    10441 gccagcacga tcagatcggc caggctcacc tggctggcgc ccttcgcgtt gaaggcggcc
    10501 tgaatgccct tcagcacctg cagcacatcg gccagctcgt cgggctcgtt caccgcccag
    10561 tccttctgcg gcgccaaggc gatgcgcgcg ccattgacgc cgccgcgctt gtccgagcca
    10621 cggaaggtgg aggccgccgc ccaggccgtt ttcaccagcc gcgcgttgga caggcccgag
    10681 gcgaggatct cgcccttcag cacggccgcc tcttcctcgc tgatcagcgg atgcgccact
    10741 gccggcaccg gatcctgcca gatgcgcggc tctgccggca cttcggggcc gatatggcgg
    10801 gcgcgcggcc ccatgtcgcg gtgcgtcagc ttgtaccagg cctcggcaaa ggcgcgggcg
    10861 aattcgtccg ggtgcttgta gaaatgccgg ctgatcttct cataggccgg atcgaaacgc
    10921 agggcgagat cgctcgtcag catggagggg gcgtggcgcc gggccggatc atgcgcatcg
    10981 ggcacggtgc cggcgccagc gccgcccttg ggcgtccact gatgggcgcc ggccgggctc
    11041 ttcgtcagtt cccactcata gccgaacagg ttcttgaaga aattgttgct ccactgcgtc
    11101 ggcgtctcgc tccagatcac ttccaggcca gagccgatcg catcgccgcc cttgccgctg
    11161 ccataggccg atttccagcc cagccccagc tgctcgatgg gcgccgcttc cggctctggc
    11221 cccaccagcg cggcatcgcc ggcgccatgg gtcttgccga aagtgtggcc gccggcgatc
    11281 agcgccacgg tctcgtaatc gtccatcgcc atgcgggcga aggtctcgcg gatgtcgcgc
    11341 gcgctggcca gcggatcggg attgccgtcg ggcccttccg ggttcacata gatgaggccc
    11401 atctgcaccg cggccagcgg atcttccaga tcgcggtcgc cggtatagcg ctcgtcggcc
    11461 agccaggtgg cttccgggcc ccaatagaca tcgtcctccg gctcccacgt atcggcccgg
    11521 ccgccggaaa agccggcggt ttgcaggccc atcgattcca gcgcgcaatt gccggccagg
    11581 atcatcagat cggcccagct cagcgccgcg ccatatttct gcttcaccgg ccacagcagc
    11641 agccgcgcct tgtcgaggtt gacattatcc ggccaggaat tgagcggcgc gaaccgctgc
    11701 tggccagcgc cggccccgcc gcgcccgtcg gaaatgcgat aggtgccggc cgaatgccac
    11761 gccatgcgga tgaacagcgg gccataatgg ccaaaatcgg ccggccacca gtcctggctg
    11821 tcggtcatca gggcgaagag gtcggccttc accgctttca gatccagcgc ggcaaaggcg
    11881 gccggataat cgaagacgcc gcccagggga tcgatctggg gcgcgttctt gttgaggatc
    11941 ttgaggtcca gccgctctgg ccaccacatc tggttatcca gcgcgcgcac gatcgccgcg
    12001 cggcttacct tgtctgcgtg aaagggacat ttgccaactt cagacatcgc gtttctcctc
    12061 gaacggtgca aagcgtctga catgtgcttg ccatggccgg gcgggttctc tccagcgagg
    12121 cttttctatc acagtttgca gcgaaggtga tcgatcataa ggctcagccc ggcgcagccg
    12181 tgccccaccc catggccaca aagcaccacg ttacagatag ttcccgcccc ggcggcgcag
    12241 gcggcgccag ggcctaggcc accggttccg ccgccagccc ccgctccatc gccagcgagg
    12301 cttcggcatg gctgcgcatc agcgcctcgg ccgcctcggc cgcgccctcg gcaatggcgc
    12361 gggccacggc cgcatgctgg gcatgggccg ccgccagcag gcccgccacg ctggcctcat
    12421 cctgcgtgtc gaaggcaaaa gccacggcag aggccagcgg aatatggtcg ttctgcgcca
    12481 gcgccatgcc gatggcgcca ttggtgcagg ccgccagcag cgctgcatga aagccggcat
    12541 tggcctcggc atagagcgcc agcgcgccct cggccagccg gccctgcgcg ccaatgcccg
    12601 cgcaaggggc gatgatggcg gccagccggg cgccggtggc ggcatgatcg ggcgcctgtg
    12661 ccaccagcct tgccgccagc ccttccagca cgccgcgcac ctgcagcgcc ccttccttca
    12721 tcgcctgatc gggccggcgg gcggcatagc cccgcttgcc cagcggcacc accagccctt
    12781 ccagcgccag cgcccgcagc gccgtgcgca ccggcatgcg cgatacgccc agccgctcgg
    12841 cggtgggaat ttcggcgatg cgcgcgccat cggcaatctc gccggtcacg atcatgtggc
    12901 gcaaggcaac catcacccgc tggccgggct tggggctgcg gggggatcgg gcaagaggca
    12961 ggttcatgcc tgcggtgttg acggcatggg cggcttcatg tcaattggat ccaatattgc
    13021 caaagcgaga ggatgccccc gatgcccaac accccttggc ctgttaacgc ctggtacgtg
    13081 gccgcccgtg ccgaggaact gggcgatgcc ccgcttggcc ggcagatctg cggcgagcgc
    13141 atggtgttct tccgcggcat cgatggcacg gtggcagcgc tggaggattt ctgcccgcat
    13201 cgcggcgcgc cgctgtcgct gggctttgtg cgcgaagggg cgctggtctg cggctatcac
    13261 gggctggcgc tggcctgttc cggcaaggtg gcgggcatgc cggggcagaa gctggccggc
    13321 tttccccctg tgcgccgctt tgccgtggag gagcggcacg gcttcctctg ggtgtggccg
    13381 ggcgcggcag aggcggccga tccggcgctg atccccgccc ttgtctgggc cgacagcccg
    13441 caatgggcct atggcggcgg ctattaccac atcgcctgcg actatcggct gatgatcgac
    13501 aatctgatgg acctgacgca tgaaacctat gtccatgcca gcagcatcgg ccagaaggag
    13561 atcgacgagg ctccggtgaa aacccggcgc gagggcgaag agatcctcac cagccgcttc
    13621 atggaagggg tgatcgcccc gcccttctgg cagatggcgc tgcgcgccgc gggccttccc
    13681 gccgaggccc cggtagaccg ctggcagatc tgccgcttct cgttgcccag ccatgtgatg
    13741 atcgaggtgg gcgtggccat ggccggcgaa ggcggcattg ccgcgcccga tgcgcgcaag
    13801 gtctacagcg tggtggtgga tttcatcacg cccgaaaccg ccaccagcca ccattatttc
    13861 tggggcatgg cgcgccgctt cggcgtggag gatgcagcgc tgaccgaggc gatccgcgcc
    13921 ggccaggggc ggatcttcgg cgaggatctg gagatgctgg agcgccagca ggccaatctg
    13981 cagcgctggc ccgacaggcg cctgctgctg ctcaacatcg atgccggcgg cgcccgctcg
    14041 cgccagatga tcgaaggcgc catcgccgcc gaaaccgcct agccgccagc cgccttgttg
    14101 ccctttggcc acagctgacg atcgcccggc ctgcccccct tgcggcccgc cgggcgatcc
    14161 attagcgtaa gcccccagca acacagccgc gcccaacggt gggcggcaaa cggcggttgc
    14221 ctcatttcca gaacagcaag cgtggcaatg gcgccgcccg gcacgatccc tttttggggt
    14281 cgcctcctgg cggctttttc gcgtgcccgg cgccggcgcc acaccgggaa gaggatgccc
    14341 tcacaggcaa ggacaacagc aaaaccatga gggacacatg aggtacaaga tttcgctgat
    14401 ggccgccgcc gcggccgcac cgctgctgat ggccgccccc gcccaggccg acagcaagcc
    14461 cgcctttggc gcgcccatcg cgctgggcga tggcttttcg ctcgatccga tcgtggaggg
    14521 ccggctgcgg ctggaagatg tgcagcagcc gcaaaaggcg ctggccgccg atgccgtaac
    14581 gatgcgcctg cgcgccggtt tcgagctgaa gaacgccccc agccatctcg ccctgctggc
    14641 cgaggcggaa ggcaatctgg ccctcgatgg cgcctacaac gccttcccct acacgctgaa
    14701 gaccagcagc cagtatcgcc ccacccatgc cgtggtggca gacccggaca gcgtgggcct
    14761 caaccgcctg cagctgcaat tcaagaccaa gccgctgacg ctgacgctgg gccgccagcg
    14821 catcaatctg gacgaccagc gctgggtggg cgcttccggc tggcggcaga acgagcagac
    14881 cttcgatgcc gtgcgcgggc aagtggcgct ggggccggtc agcttcgatg gcagctttgc
    14941 cgaaagccag cgcagcatct atggcaccga tggcggcccg cgcgtggcct atgatggccg
    15001 ctttgccttc ctcaacgccg gcatcggcaa ccagatcgcc acgctgaagg gctttgccta
    15061 tctgctggac tacaataatt cggccttcca gaacctgaag gccacccgcg gccagctcga
    15121 cagcagccag acctatggcc tgcgcaccac cgccaagctg ccgatcgaga agggcgtgag
    15181 cctggcgctg gccggcagct atgcccggca atacagctat gccgccaacc cgcgccacta
    15241 tgccgccgat tactatgcgg tggaaggcaa tctggccgcc tgtggcttca cgctgaccgg
    15301 cggctacgag atgatgggcg ccgatgccca tgcggtgggc ggcccgtggt cggtgcagac
    15361 gccgatggcc acgctccacg ccttcgatgg ctgggccgat gtgttcctca ccacccctgc
    15421 ccgcggcctt ggcgacacat atgccggcct tgcctatgcc ttccccaagg cggtgccggt
    15481 gcccggcctt aacgccaagg tcatctacca ccagttcgac agcgacatcg gcaatgtgaa
    15541 ctatggccac gaatgggatg ccgccatcgg cttcaagacc ggcttcctca actggctggt
    15601 gaaatacgcc gattaccggg ccgtgggcgc ctcggcactg ggggcgggca ccaccacccg
    15661 caagttctgg ctgcagagcg aattcgcctt ctgaaccaat gccggcccgg ccccgaccgc
    15721 ccctgcccct caggccggga agaagcggtt ggccggccgg gccagcccca gatgatcgcg
    15781 cagggtggtg ccggcatatt cgcggcggaa caggccgcgc cgctgcaatt cgggcaccac
    15841 cttgtcgcag aaatcctcca ccccgcccgg cgcgtgggag aacatcacgt tgaacccgtc
    15901 ggagcccttc tccaccagcc attcctccat ccggtcggca atggtggcgg ccgtgccgac
    15961 aaaggccagc ccggcatagg ccccggccct ggccgccagc tggcgaatgg tgacgcccgg
    16021 ccgggccccg gcctcgatga agcgatcgcg cccggatttg ctggcattgc tttccgggat
    16081 cggcggcagc gggccatcgg gatcgaagcc cgagacatca taatccagcg cgatcgacag
    16141 cgagcggatg ccgctgtcgt aatggacgag gctgtcgagc cgggcgcgct tggcctgcgc
    16201 ctcctcctcg ctgtcgccca ccaccacaaa ggcggccggc aggatcttca gactgtctgc
    16261 cgcgcggccc agcgccgcca tgcgcccctt cacatcggca tagaaggcct ggccgcgcgc
    16321 cagcgtttcg gccgcgccaa agatcacctc ggcggtttcg gcggccagct gccgccccgc
    16381 ttccgaggcg cccgcctgca cgatcaccgg ccagccctgc acgggcctcg cgatattgag
    16441 cggcccccgc accttgagga aaggcccctc atggttcagc acatgcagcc ggtcggggtc
    16501 gaaatagacg ccgctttcgg catcgcgcac aaaggcatca tcggcccagc tgtcccacag
    16561 gccggtcacc acatcgtgga actcacgcgc gcgggcatag cgggcggcat gttcgagatg
    16621 ttcgtcgcgg ccgaaattat gggcggtttc ggggttgccg gtcgtcacga tgttccagcc
    16681 ggcgcgcccc tcgctcagat gatcgagcga ggcgaagcgg cgggcgatat gatagggctc
    16741 gtcgtaggtg gtggaagcgg tggccacaag gccgatccgc tcggtggcgg tggccagcgc
    16801 cgacagcagg gtgaaaggtt caaagctggt aacggtgtgg ctgcgcttca gcgcgctggc
    16861 cggcatgttc agcaccgcca gatgatcggc catgaagaag gcgtcgaacc gggccgcctc
    16921 cagcctttgc gccaggcggc ggatcagcgg gaagttgaaa ttgccatccg gcgccgctcc
    16981 cggatagcgc caggcgccgg tgtggatcga gatggggcgc atgaaggccc ccagatgcag
    17041 ctgacgatca gatgccatat gcagatcctc cttggcgggc aggcgcgccc gtttgcgcaa
    17101 acgccaaggg cggcggccgg gttggcccgc cgcccctgtg ctctcagaag tccttgcggc
    17161 ctcagaaatc cttccgaagc gtgaagccgg catagcgggc atcgtcacgc ggcacaaagc
    17221 gcaccgtgcc gccgatgttg ccattggcca gatagtcgga ataatgggtg ttggtgaggt
    17281 tcttgatata ggccctcagc tgccagccat tgtggccgat cagcgccgcg ctgatgttcc
    17341 agatggcata ggccggctgg atggtgttgg cggtttcggt cagctgatac tgggtggcgc
    17401 tcttcagcga gacatcggtc tgcagttcca gatccgcccc gcgcgccacg ccaaagcgat
    17461 aggaagcgct ttcataaagc ttccacttgg gcgcgaaggg cagcggctgg ccatccacat
    17521 tgcagctgtt gggcgtgccg gccgggcagt tgaaatgcag caccttggca tcgtcatagg
    17581 ccagcgaggt gtcgaaggag aggcccggga tcgggtggaa gccaaggtcg atctccaccc
    17641 cgcgggtgcc cacccgcccg gcattgacca gccgcgtgac cggcgatccg cccaccacat
    17701 cgttgaaatt ggcctgatag ttgttgatca ccgtgctgaa cacatccacc gcataggtga
    17761 tctggtggcc cagcgtggaa cccttggcgc ccacttcgaa ggcgttggag gtttccggcg
    17821 ccagggtgat ctcatcacgc gcgcgcatgt tgaagaaggc gtcgaaggcg ggccccttgt
    17881 agccgcgcga ccaggtgaaa tagatctggt tgccgcgggc aaaatcatac tggagcccgg
    17941 cgcgggcagt gtcgccgcgc gcgtcggtgg agccggcaga ggcgtgatag gcgccgatgc
    18001 cggtcacatc cagcgccgag gcgccgctgt tcgtgggatc gttggtggag gtgcgctggt
    18061 ggtaataggc tagcttgtcc cagctgctgc gatagcccag gatagcgcgc agccggctgg
    18121 tgaagttcac attggcctcg ccgaacaggg cataattgtt cgagcgcgtg ccataactgg
    18181 cgatgccctc ataaggcgtg gcggtgcccg agatcagctt ggtcacatcg cgctgatagc
    18241 gctcgtcgtc ctgggcgtag aggtagtagg cgcccaccac ataatcgatg aagcggccct
    18301 tggggctggt caggcgcagc tcctggctga actggtgcgc cgacagatcg ccccggtcgc
    18361 tgccctgata gagcgaggag gtgagctggc cgatctggtc gtaatcctgg tgctggtggt
    18421 tggtccagcc gcgataggcg gtgatcgagg tcagcgtgta gccattgttc agccgccatt
    18481 cgccggtcag cgaggtgccg tagttgcggt cgcgcacatc gctgtcgtag ctggaagtga
    18541 cagtgcggtt atccgccccc agcgccacgc cctgcccggc aaaatagctg gccagcgcgc
    18601 tgctggtgga aaccgcgccg gtgggatagg cgctctggct ggcgctggcg aagacgccgt
    18661 tgggcacggt atcgtgcgag cgcaggtaat cgccggcgag tgtcaatttt acattgggcg
    18721 cgggcgtgta ttgcagcttg gcgcgcaggc ccgtgcggtc gtagccgttc acttcattgc
    18781 cggttgtgac gtttttcaca ttgccgttgt aatgggtgta gagcccgtcg atgcggccgg
    18841 tgaggttttc cgccagcggg ccggtgatgc ccgccttcag gcgatattcg gtgtcggtgg
    18901 tgatgcccgt ctcggcatag ccgccaaggc gctccgaggg cgcggccgtc accacgctga
    18961 ccacgcctgc cgaggcgttc ttgccgaaca gcgtgccctg cggcccgcgc aggatctcca
    19021 cccggtccac ctcgcccaca tccagcgtgg cctggcccgg gcgcgtcagc accacgccat
    19081 cgaccacggt ggacaccgaa ggctccaccc ccggcgaggt gctgatcgtg ccgatgccgc
    19141 ggatgaacac ggtgcggtcc ttgttcgagg caccgttgcg gaaatcgatc gtcggcacgg
    19201 cagcggccag atcctgcaga ttgtgcagcg cccgatcgtt cagcgaggcg cccgacaccg
    19261 cggtgatggc gatcggcaca tggctgaggc tttcgttgcg atagcgcgcg gtaacgacga
    19321 tatcgccatt ggcctgatcg gcagcgggcg cagcggggac aggcgcagcg ggggcagcct
    19381 catcggccag ggcaggggcg gccggcagca gcagggcgct ggccagcaac agggcgcgcg
    19441 tgggagagcg gcgagacatc gggtttcctc cgtattgaac ctttgcccgg cctattagtc
    19501 aacctatttg gtagagaaag gaagtttatc tttacccgcc aacagatccg ccctcggcca
    19561 ccgggcaaat gcggcccagc atcagctgga tccagcccag cgccagcaga taggaaaggc
    19621 aggcaaagcc aaacagcggc gcatatccca gccccgcggc cagcacgagg ccgctgatct
    19681 tggcaatggc aatgcccccc agattgccgc agaacgagcc aaaccccgtc acccgcccca
    19741 ccgcattgcg cggcaccaca tcggcgatca gcccgaacag gctggtggaa aagccctgat
    19801 gccccgccag cgccagcgcc atgatcgcca ccgccgccca gatcgcctgg ctgcccagcg
    19861 ccagcggcac cggcaccacg cacagcgcgc tggccagcat cacccccttg cgcaccgcat
    19921 tcaccgacac gccccgctcc agcagccgcg ccgcaacgaa gcccgaggcc aaagagccca
    19981 ccgccgcccc gccataggcc agcgccagcg gcagcgccaa acccgtgccg ccaaggccga
    20041 actggcggtg gaaataatcc ggcatccaga acagcagcag ccaccatgtg gcatccgaca
    20101 gcaccttggc gccggcaatc gtccatgtct ggcgctggcg caggagagag ccgccggccg
    20161 cctgcacgct ggcagccccc ctcagcgcca gcccgcgcgt ggccagcagc cacagccccg
    20221 cccacaccag ccccaccgcg cccgacagca cgaatgtggc ccgccagccc caggccgccg
    20281 ccacaaaggg gatcgccagc ggggccagca cggcgccggc gctgttcatc gcattggcca
    20341 tgccaaagcc ggcgctgcgc tggttggcgg ggaagatggt ggcgatggtc ttgatgccgc
    20401 tgggcgtgcc catcgcctcg gtggtgccca gcgccacgcg cacggcggca aagccggcca
    20461 tggagcgcac ccagccatgc gccatcgccg ccacagacca ggccgccacc cccagcggat
    20521 tggcccattt cacccccagc cggtccacca gccagcccgc cgccagaaag ccaaacgccg
    20581 cgcccgcctg gaacagcgcg gccagcgtgc cataatcatc gtcgttccag tgaagatcgg
    20641 ccgagatcac cggcttcagc acggcgatga tctgccggtc caccagattg aggatgcccg
    20701 ccgtcaacgc cagggcgaag agcaggttgc ggcggcgggg ggaaagggca tcaggcaggg
    20761 gatgaggcag tgcatcgggc atggcgccgg cgatagacgg gcggccagcc cccggccagc
    20821 aagtttttct actgataagg tagagtatgg ggcggcgcgg cggccgcccc tcgccggttc
    20881 agaacggcgc gtggtctatc cgcggcagca cgtagcgggc aaacagatcc gcctcgcgcg
    20941 catggggata gcccgacagc acgaaggcat cgatgcccgc ctgccgatag gcctgcaact
    21001 tggccagcac ctgatcggga tcgcccacga tcgccgcccc gcagccagag cgcgcccggc
    21061 cgatgcccgt ccacagatgg tcttccacat agccatcgcc ggccgccgcc tcgcgcagcg
    21121 ccacctgcgc ggccacgccc accgattgcg tatccagcga ctttgccttg atggcagcgc
    21181 cggtggccgc atccagcttg ctcagcagcc ggtctgccgc cgcgcgcgcc tcggcctcgg
    21241 tttcgcgcac caccacatgc acgcgatagc catatttcag ctggcgcccc tcgcgcgcgg
    21301 cgcgggcctt catgtctgcc accaccgcgc gcacattctc cagccggtcg ggccacatga
    21361 gatagacatc gcagcccttg gccgccacct cgcgcgccgg ctccgacagg ccgccgaaat
    21421 agagcggcgg gcaggccgtg ccggtgagga tgcgcgccgg atccagcttc aggttccaga
    21481 agcggccctg gtgatccagc ccctcgcccg ccagcagcgc gcgcaggatg gtgatcgcct
    21541 ccagcgtgcg ttcatagcgc gccgggccat ccagcacctc gcccggcatt tccgaggaga
    21601 tgatgttcac attcagccgc ccgcccagga tctggttgat cgtggcgatc tggcgggcca
    21661 gctgcggcgg ccacagctcg cccacgcgca ccgccatcag cagcttgatg cgcttcacct
    21721 gggtggcaat ggccgcggca aaggcggcgg tgtcgatccc cagttgatag ccgttgggca
    21781 gcaggatatt gtcgaagccg gcctcttcgg cggccagcac gatattgcgg caatgctccc
    21841 agctggattg cagcgccggc tcgttctggc cgaggaattc gtaatcatca tcgcacaggg
    21901 ctgaaaacca gctgatttct acaggcgttg tcacggcatg cgttctccgc gcgatgcttc
    21961 gagcaccgcg taccattcgt tgcgagtcca tcggatgcgg aaggcatcgc ccgagtcgag
    22021 aattctctcc ggcgtctgcg tgcccacgat gggcacgatt ttcgccgggt gcgccatgat
    22081 ccagctcaaa gcggccgccg ccgcatcggc ttcgtatttt gccccctgcg cggccagcag
    22141 ggccgcggcc gggtgatccc cccgggccag cctgcccccg cccagcggcg accaggccag
    22201 caccgccatc tcgcgcgcca tcgcatcatc cagcgtgcca tcataaagcg gggcataatg
    22261 ggcgggcgag aattccggct gggtggacac cagcggcagg ggcaggaagg cctgcagcgc
    22321 ggccacttcg gccgggctgt agttcgagac gccgacatgc ttcaccttgc cggccgccac
    22381 catggccgcc agcgcctcgg ccacctcgcg cgggtgggcg aggaaatcgc ggcggtggat
    22441 ctggtaaagc tccacgctgt ccacccccag ccgggccagc gaggcatcga tcgcgcctgc
    22501 cagataggca gtggtggaat tatagggcac gcccggccag atgccgccct tggtggccag
    22561 gatcatgcgc gcgcgcagat ccgggcggcg ggccagtgcc tggcccagca gcgcctctgc
    22621 cccgccaaag ccgatcggct cgcccgcgcc atagatgtcg gcggtgtcga acagggtgat
    22681 gcccgcttcc agcgccgcct cgatgcgggc aatgatcgcg ccgatgtcat cctgcccggc
    22741 ggggctggca atgcgccaca tgccccaggc aatgggaaag acggacaatc cgctcttgcc
    22801 aagcgggacg ggatgcgttt ctacgcaaat ggttgagttt tgatcggagc gcgtgtcagt
    22861 catgcccgct ccatgtcaca aagggcacac gaatggtaga gacgatccgc tacggcatca
    22921 tcggcacagg gatgatgggg gtggagcata tccacaatct ccagatcctg ccgggcgccg
    22981 agatcgtggc ggtggccgat cccgatgcca cctcgctggg ctgggcgcgc aaggcgatcg
    23041 gccatgaggt ggccgaattt gccgatgccg ccgcgctggt caagggcgcc gatgtggatg
    23101 cggtggtggt cgtcagcccc aatttcaccc atcgcgcggt gctggaaccc ctgttcgatg
    23161 ccgatgtggc gatcctgtgc gaaaagccgc tgtgcaccac catggccgat gcccgctggg
    23221 tggccgccaa ggccagggcc caccaccgcc ccttctggac cggcatggaa tatcgcttca
    23281 tgccgccggc ggccgaattc atcgcccagg tgcatggcgg ccgggtgggc gcgctgcaga
    23341 tgctttccat ccgcgagcac cgcttcccct tcctggtgaa agtgggcgac tggaaccgct
    23401 ttgcccgcaa caccggcggc acgatggtgg agaaatgctg ccatttcttc gatctgatgc
    23461 ggctgatcgt ccggtccgag ccggtgcgtg tctattgctc gggcgcgatg gatgtgaacc
    23521 acctcgacga gcgctatgat ggccagcgcc ccgacatcat cgacaacagc ttcaccacca
    23581 tcgatttcgc caatggcgtc agggcgatgc tggatctgtg catgtttgcc gaaggggccg
    23641 aacatcagga agagatcagc gccaccggcg acagggcgcg gctggatgtg ctgatcccgc
    23701 cgggcgatct ggtctatgcg ccgcgggtgg actggcgcgg ccccaaggcg gtggaacgca
    23761 cccatgtggc ggtggacgag gcagcgctga aggccggctc gcaccatggc tccacctatt
    23821 accagcacga ggcctttgcc cgcgcggtgc ggggcgaagg gccggtgatc gtctcggccg
    23881 aggatgggct gatggcggtt gccatcggca cggcggccga gatcagcgcg cgcgaacacc
    23941 gcgtggtgga catggccgag gtgctggagg cctgaagcgc ttcgacgacc taagtgccga
    24001 ggggcctaga gcccctcccc gccaatccag tgcgtgttgc tgagcatgcg cgccagatgc
    24061 cacacctgcc ggcggatatc gggcacggcg atgatctccc aggcctcgcc cttggtcacc
    24121 gggcccacgg catagagatg cggctggcgg cgcccctgcg catccagcac cgatccggtg
    24181 tgatcgacat cgaggcccag ccggtgcaca tcggcccgcg cccgcccctc ggccaccagc
    24241 gcacgcagca gcggatgatc gctgcgggtg atgtcgccct cgggccccgt gcacagcacc
    24301 acccggccca catccagctg ccgttccggc ccgccgccgc gcggctgcca gcgcacatgg
    24361 gcgccatcgc cccgcggctc tgcccccagc aggcggccgg ccacgaaatc cagccggcca
    24421 tccgcgcgca gccggtcgat cacctcggcc acggggggcg ccaggcggtg ccggtgcaca
    24481 tcccaccagg gccgcagatg gcgcaggaaa cgcgcctgcg ccgccgcatc atgctggcgc
    24541 cacaggcttt gcgtgtgggc gcgcaattca tccaccgcat ggcgccagcc gacatggccc
    24601 gcccgccggc gcaactggcg cagcagccag gagccgcgcg cctctggccg aggcacggcg
    24661 cccaccaccg gcccttcggc ggcatgcgcg cgcggcgcca gcccccggcg cgagagcgcc
    24721 gtgatgcgcc cgcgatagcc cgccttttcc aggctgagcg cgacatccac cgccgtcagc
    24781 ccggtgccca ccagcagcac atggtcgatc ccttccagat cctgcgtcac cgccgccgac
    24841 caggggttgt cgatcagcac cggcggggca aggccggcca gcaccggcag gcgggccggc
    24901 gcgaaattgc ccagcgccag caccaccgcc cgcgccgcga tcacctgccc gctggccagc
    24961 cgcagcgcgg catgatcggc gtgccagtcg atccccagcg cctcttcctc gcggatggca
    25021 aagcggcccg gcgccgcggc cagcgcggcc agcaactgct cgcgcagata ttgcccatag
    25081 gtgaggcggg gggcaaaacg gttggcctgc tcctgcgagg aaaagcccat ccagcgcagg
    25141 aaatggcccg gatcatcggg aaaggcgctc atgttggcag cgcgcacgtt gagcagatgt
    25201 tccggcgcgc gcgtgccaaa ggcaaggccg cgcgccagct tttcgcggtc gcgctccacc
    25261 agcaccaccc gcgcgcccag ccgcaacagg ttgatcgcca gcaaagtgcc ggaaaagccc
    25321 gcccccacga tcgcgatcgg caggtcggcg ccgttggcgg gggatgggga tgcgggcatg
    25381 ggatcaggct cctgggccgc gccgcggccg tctttgatgg tgggtgtcat gccgcatcat
    25441 cgggcatatc tcaacttttt tggtagatat taagcgccgc gaatgcatcc cctttcgcag
    25501 cgaagttttg cttcgcccat ggcaaagaac gcttggctga aaaatcccta ccacattgat
    25561 agacattatg gcgaagcagg cacatcccgg cagccaccgg tatttcgcaa aggcgatgcg
    25621 cccgcaagaa cgaaagccat ggcaggggga aagcttatgg gttttatcgg gcatcttgat
    25681 ctgctgcacg cgctggccgg cctgttggtg ggcctgctgg tggggctgac aggcgtgggc
    25741 ggcggatcgc tgatgacgcc gctgctggtg ctgctgttcg gcgtggcccc gcaaaccgcg
    25801 gtgggcaccg atctgctcta tgccgccatc accaagatca ccggatcggc cgtgcatggc
    25861 tggcgcgaca cggtggaatg gagcatcgtg cgccggctgg cctatggcag cgtgccggca
    25921 gcgattgcca cgctggcggt gctggcgcat atcggccacc ccgatcctgc cacgcagaac
    25981 atcatcctgc tggtgctggc ggtgctgctg gcgctgactg cgctgagcgc cttcttccgc
    26041 aactggctga tccgctttgc cgccgatctc gatccactcg atccgccgtt caaggcggtg
    26101 gtcggcacgg tggtgctggg ggcggtgatc ggcgttgcgg tgtcggtgtc ctcggtgggc
    26161 gccggcgcga tcggggtaac cgtgctgctg atgctctatc cgcgcctgcc ggtggtgcgc
    26221 gtggtcggct ccgatatcgc ccatgccgtg ccgctggcgc tgatcgccgg ggtgggccac
    26281 tggatcatcg gcgatgtcga tggcgcgctg ctcaccaacc tgctggtcgg ctcgatcccc
    26341 ggcgtggtga tcggcagcct gctgtccaca cgcgcgcccg acaaggtgct gcgcccgctg
    26401 ctggcggtag tgctggcgat ttcgagctgg cagctgttcc tcaaaaccct cagccacgaa
    26461 cacaccgcgc ccaggcccgc cgccagcccc acccgctaag cgcgcgccgc cccatcgcca
    26521 ccaagcacag gatatttgcc atgcccatga caatgccgcc acgccggctg gcccccgccg
    26581 ccggcctgct gctggccctg gccagcccgc ccgcccatgc caccgacggc tatttcctca
    26641 acggcgtggg cgccaaggcc gaaggcgagg ccggcgttgc catcgccctg ccgcaagatg
    26701 cgctggcaat cgccgccaac ccggccgccg ccaccgaagt gggccaccgg ctggacatcg
    26761 gcgcgcagat cttcgtgccc gatcgctcgg ccacgatcag cgggaatggc gccgggctta
    26821 acgggcgcta tggcggcaat ggcgccaacc ccttcctgct gcccgatatc ggctatgtgc
    26881 gcccgctggg cgagcggctg gcgatcggca tcgccatcta tggcaatggc ggcatgaaca
    26941 ccacctacaa ggccaaccct ttcgcaagct tcggcgccag cggcccggcc ggggtcgatc
    27001 tcaagcagat cttcatcacc ccgaccctcg ccgcgcgcgt gctgccgggc caaagcctgg
    27061 gcatctcgcc gatcatcgtc gtccagagct tcaaggccag cggcctgcag cctttctccg
    27121 cttattcgga agacccggcc catttcacca atctgggcga aagctggtcc accggcgctg
    27181 gcgtgcgcgt gggctggcag ggccatttcg gccccgcgct gacccttggc gccttctatg
    27241 aatcgaaagt ccgggccagc catttcggca aatatgccgg cctcttcgcc ggccagggcc
    27301 gcttcgatgt gcccgccgcc tggggcgcgg gcatcgccgt gaaggccaat gcccgcctca
    27361 cccttgccgc cgatgtgaaa cgcatcgaat atgccggcgt tggcgcggtg ggcaacgggc
    27421 tgggcgcgct gtttctgggc gtgccgttcg gggccagcgg cgggccgggc tttggctggc
    27481 gcaacatcac cagcgccaag ttcggcatca gctatgcggc cagcccgcgg ctgatcctgc
    27541 gcaccggcta tggctggacc ggcaaccccg tgcccgcttc gcaaacgctg ctcaacatcc
    27601 tggcccccgg cgtggtgcaa aaccacctga cgctgggcgc cacctggacc acgcgcggcg
    27661 gcgttgagct gaccggcttt gcgatgcatg cgctgggcca cacggtctat ggcaatggct
    27721 ccatccccgc cgcttttggc ggcggcgaaa gcgatgtggc cctgggcgaa accgcctttg
    27781 gcctttcggg cggctggaaa ttccggtgag cctggcgctg gccggccatc tgcccaatct
    27841 ggcaggcggc gcgctgatcg gcctggcagc gggcgccctg cggctgacga gcggccatat
    27901 cgccggcatc agcggcatcg cccggctggc cctgcgcggc cccgaacgcg gctggcgcct
    27961 ggccttcctt gccggcctta tccttgccgg cctcatccct gccggccctg ctgccagcct
    28021 caccgccggc ctggccgaaa cgcccctgcc caccctggcc ggcgccggcc tgctggtggg
    28081 cctgggcacc ggccttggca atggctgcac cagcggccac ggcatctgcg gcctggcgcg
    28141 cctgtcgcgc cgttcgctgg tggcggtggc ggtgttcatg gccactgcca tgctgacggt
    28201 gctgatccgg caccacaccg caggcatctg gcaatgagcc cggccagcgc cagaggcagc
    28261 acctccacgc tggccgcgct gggctgcggg ctgctgttcg gcgccggcct actgctatcg
    28321 gggatgacca gcccggcccg cgtgctgggc tttctcgaca ttaccggcca gattaccggc
    28381 cagtgggacc caagccttgc ctttgtcatg ggcggcgcgc tggcggtagc cgcccccctg
    28441 ttcgccctgg cccgccggcg cggagcagcg ctgaacggcc gccccttggc agaccctgcc
    28501 agccgcctcc ccgatgcccg tctgctggcg ggcgctgcgc tgttcggcct gggctggggg
    28561 ctggcgggcc tgtgcccggg gccagcgctg gtcgatctgg cgctgcgccc atggccggcc
    28621 ctggtcttca ccggccccat gctggccggc ctgctgctca gccggcgcga ctgatccggc
    28681 catatcgaac agattgagcg cgccgatcgc cagatggagc gcggcttgcc cggactgata
    28741 gcccgggttc cggaaggggc agcccgcccg cgtcgttgct gctgaaggaa ggcggctgca
    28801 cggcaggtgc gaaaccacca acctaaatca tggggaaata tggtgagccc agctggattc
    28861 gaaccatcga cctactgatt aaaagtcagt tttcagggcc ttttgtgacg cgatggattt
    28921 tccattaatg aaaaaattac aatgcattat gtcgaatctc gtttcacggt gtagcaggat
    28981 ttttgattgc tggtgcttgc tatgtgcttg ctagaatgct tactggcacc ccgacacagc
    29041 ggcgagaaag cacccatggc gtccatcacc aagcgcaccg tagacgcagc ccaacccggc
    29101 acccgcgaca gcttcttatg ggatgacgag attgccggct ttggcctcaa gatcaccccg
    29161 gcgggcggca gacctacatt tatcaatatc ggatgacgcg ccccggcatg gccaaggcct
    29221 gccccacccg gcgccatacc atcggcagac atggcaatct gacaccgctt caggcccgcg
    29281 cccgcgccaa ggaactggcc gccatggtgg cgcaaggcat cgaccccggc cagcttgaaa
    29341 aggaccgctt caccgctgcc gacaatgccc ggcgcgcggc cgaggaaaag gccagggcgg
    29401 aaagcctgct tgcctttgac cggctggccg aaacgtggct cgaccattat gaaaacgaga
    29461 aggagcgccg cccgtccggc gtggccttgg ccaagctggt ggtgaaccgc tatcttgtcc
    29521 ccgcgctcgg caaccggcct atgccgcaca ttggccgcag cgacctaaac cccatcctag
    29581 acagcatccc caccgccaag cgcggaatgc gccgggctgt tttcgcctat ggctcgatcc
    29641 tgtttggatg ggcgcacaag cgcggcgaca ttgccgaaaa ccccttccta tccatggtga
    29701 agcccgaggc gccaaaggcc cgcgaccggg ttttgaacga tgacgagctt gccgccatct
    29761 ggaaggccac cgccaccctg tccgaaccat tcggcccgtt ctttcgcctg cttgtcctga
    29821 caggccagcg ccaccgcgaa gtttccgaca tcagttggac cgaactggac ccaggcggcg
    29881 cgctctggac cattcccgcc ggccgtgcga agaacggcgc cgcgcatctt gtgccactat
    29941 cgcccgccgc catagccgag ctggacgcgc tggccctcgc ccagcacatc aaggccaaag
    30001 ccaaaaagcc cgatggcgcc aaatggccga ttgtgggctt tgcactcacc accacagggc
    30061 acagccccat cagcggcatc agcaaggcca aagccgcact ggacaaagcc ataaccgagg
    30121 cgcgcaaaga tcagggtggc gccattcccg cttggcgcat ccatgacttg cgccgcagcc
    30181 tcgccacagg ctttcagcgc cttggcgtgc ggtttgaagt aaccgaggcc acgctcaacc
    30241 acatcagcgg cgccaagggc ggcgttgccg gcatctatca gcgccacgat tgggccgagg
    30301 aaaagcgcgc agcccttgac gcatgggcgc ggcatgtcgc cgcaatccgc caaccggctg
    30361 accaggccaa tgtggtgaag ctgcgggcat aatcgcggat ttgctgcggg gtatctcatt
    30421 tgccggcgag aacggcgaga accggagaac gcagcaggca acccactgta ttactagggt
    30481 aaatcctgtt ctcgcgtaag gaaggggccg ttctcacccg acaaaggtga gaacggcccc
    30541 ctcgcgatat gcccgaagcg agccgcgcgg ctagcggtca gcccatcaat tgcggccaat
    30601 gacgctgccg ccagcttatc caattgaggg caaccatgaa ggcctgacat tgggacaaag
    30661 ccgccgtcca tgggtctacc ggcgaatggc gggttcggcc aaaacagacg ttggtttgtg
    30721 gggttaaaaa cggctaggat gggtggtaga gcggtctgtc accggcgccg tgccgatgct
    30781 gccacgccat acgccgaccc agcccggttg cgccggccct gccttggcca aagccccttc
    30841 aaccccgagc agcagcaccg cacccgcaac gagcgtaaac cacacggtgc ggcgcatgaa
    30901 gacggcatca ttcgcactcc tgcgcggtgg ccatgatgga attgatcgca tctccagcca
    30961 ttgcggccgc ctcggcgggt gacttgccca gatgggcagc ataaagggcc gtaagctcgc
    31021 tgcgcgcggc ggtgatatcg gccggcgtga cgccgcgtcg gcggggcgcc ttgatgcccg
    31081 gatcgcgatg cgccaacccc aggcgcgcgg tatccagcgc cgtctcgtag acgcgatagg
    31141 cggcgaaacg gatggtgccg tcgcaaggcg gccaagcggc gttgagcttg ccatcgagtg
    31201 cggtgagcat gggtgtgggc g
//
LOCUS       DASRYE010000311         6297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_34631,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000311 DASRYE010000000
VERSION     DASRYE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34631"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFF98_16015"
     CDS             <1..196
                     /locus_tag="VFF98_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HZU65194.1"
                     /translation="AHGGPAAAVKRITTADYPTPARRPANSRLCGAKLAAAYGLALPD
                     WRASVAGVVARLLAEQEKAA"
     gene            193..1077
                     /gene="rfbA"
                     /locus_tag="VFF98_16020"
     CDS             193..1077
                     /gene="rfbA"
                     /locus_tag="VFF98_16020"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZU65195.1"
                     /translation="MKGIILAGGSGTRLYPMTLALSKQLMPVYDKPMIYYPLTTLMLA
                     GIREILVITTPQDQGAFRHLLGDGSQWGVEIRYAVQPEPRGLAQAYTIGADFVAGGPS
                     CLVLGDNIFFGHGLPQVLAGARAGLAAGGARVFAYQVADPERYGVVEFDSARRAVSIE
                     EKPAAPKSNWAVTGLYFYDAEVVEIARSIQPSARGELEITDVNRAYLAAGRLSVEVMG
                     RGYAWLDTGTPESLLEAAQFVATLEKRQGMKIACPEEIAWRMGYIDDAGLEQAAARLG
                     KGDYAAYLRRLLQIGQLG"
     gene            1260..2297
                     /locus_tag="VFF98_16025"
     CDS             1260..2297
                     /locus_tag="VFF98_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZU65196.1"
                     /translation="MPKITPVILCGGSGTRLWPRSRKAKPKPFLPLVGDYTLFEEALR
                     RTADRAIFTAPVIVTGTAHLEHVESQLAFAPDAQVIVEPAARNTAAAIALAALRLPED
                     AVMLVCPSDHHIGDPAAFVAAAQAAAGLAAEGWLVAFGITATAPETGFGYVKRGEAIG
                     AAGFRVDRFVEKPDLERAITFLAEGSYSWNGGIFAFSAGTFLAELAAHRPAIAEQARK
                     AVAAGHEEGRRFHPDAALFAGVESDSIDYAVMEKTSRAAMVPAAMAWSDIGNWQALHA
                     ARLQDDRGNAVLGAVELVDCSNVLVESDGPRVSVIGLENVMVVVDGGEVLVTTAAGAQ
                     KVGKLAGAANQ"
     gene            2300..3157
                     /locus_tag="VFF98_16030"
     CDS             2300..3157
                     /locus_tag="VFF98_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HZU65197.1"
                     /translation="MAAAISPALPVRLVEKPWGVEVLPAPFAGDPRGAGRRIGEIWFE
                     PPAAVPELLLKYIFSSDKLSVQVHPSDAQTLAQGLGRQGKEECWLVIDAGPDARLGIG
                     FHEPVEPEAMRAAALDGSIEQMLVWHQVQAGDFFYIPANTVHAIGAGVSVVEVQQNSD
                     ITYRLFDYGRPRALHLEESLAVALGTPYDLARHHRRLGPGGDAQLVEGPYFRLDRVEG
                     LPDAGLRAGLAARYGHAPLVVLPLAGALTVMGATVAAGGCAIASTLADIAFAPGGQCL
                     LAQPLGVSA"
     gene            3352..4338
                     /locus_tag="VFF98_16035"
     CDS             3352..4338
                     /locus_tag="VFF98_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZU65198.1"
                     /translation="MPAARAPDGAALLARGRAVIAAEGQGLALLAAGLDGRFADAVAL
                     IAAGRRLVLAGMGKSGHIARKLAATFAATGTPALFVHPAEAAHGDLGMIAAGDVLLLL
                     SNSGETRELRPLLAHGRALGCKVIGMAGRAGSFLMAAADVGLCLPQVAEACAISCAPT
                     VSSTMQMALGDALALAVMDLCGVAPARLRALHPGGALGLQLARVDEVMQGPGALPLVE
                     EATPMAEVISVITRGRFGLAGVTDGAGRLVGAISDGDLRRHIGQLAGAVAGAVMSRAP
                     VWVPAGMLAREALALMGQARISAVFVMAEGAGGAGRPLGMLHVHDLLRLGAA"
     gene            4342..5199
                     /gene="kdsB"
                     /locus_tag="VFF98_16040"
     CDS             4342..5199
                     /gene="kdsB"
                     /locus_tag="VFF98_16040"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZU65199.1"
                     /translation="MALLRDTEAWPLGKASVPGGACAVVIPARYGSTRYPGKPLALLR
                     GGSGEARPLIRRSWEAARAGLAEMGMPARLLVATDDPRIAEAVAAFGGEAVLTPPDCR
                     NGTERCAALLEALPASVEVVVNWQGDAPLMPPAALAAVVAGLGRDAGAVMATAALACS
                     AQVHAHLLADAAAGRVGGTTVVVNGRGRALYFSKRVLPFAPAAGHAAVRLHLGLYAYR
                     RAALAAYAALPPSRLEQEEGLEQLRFFEGEGGIAVVDLAEPGWPVIELNNPEDAPLIE
                     AVLAQRGIA"
     gene            5196..6032
                     /gene="kdsA"
                     /locus_tag="VFF98_16045"
     CDS             5196..6032
                     /gene="kdsA"
                     /locus_tag="VFF98_16045"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZU65200.1"
                     /translation="MSAVALAPGLTFGNDRPFVLIGGMNVIEGRDSLLRVAEHFARTC
                     ADLAIPLVFKASWDKANRSAITSFRGPGLEEGLALLAEVKAAFGLPICTDVHEPWQAG
                     PVAEVADIVQLPAFLARQTDLALALAGTGRVVLVKKPQFLSPAEMPHILAKLAQGGAP
                     GALLGERGTSFGYNNLVVDMLGMDLMKPMAPVVFDVTHALQRPGGRPDSADGRGSQAA
                     ALARAGLAVGIAGLFVEAHPDPAAALCDGPSALPLGDLAPFLRQMQAVDQAVKQAVKQ
                     AV"
     gene            6036..>6297
                     /locus_tag="VFF98_16050"
     CDS             6036..>6297
                     /locus_tag="VFF98_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65201.1"
                     /translation="MAGAPPFLRMPPFPGARVRQLAWRRPRPLPLDEAALAEAAALAA
                     ALREEQVGGDFWGARPVGVAAASGGVVRAMPRGADPWHVAGAA"
BASE COUNT          826 a         1926 c         2487 g         1058 t
ORIGIN      
        1 ggcccatggc gggccggcag cggcggtgaa gcggatcacc acggcagatt atcccacccc
       61 ggcgcggcgg ccggccaatt cgcggctttg cggcgcgaag ctggcggcgg cctatgggct
      121 ggcgctgcct gattggcggg cctcggtggc aggggtggtg gcgcggctgc tggccgaaca
      181 ggagaaggcg gcgtgaaggg catcattctg gccggtggca gcggcacccg gctctatccg
      241 atgacactgg cgctctccaa gcagctgatg ccggtgtatg acaagccgat gatctattac
      301 ccgctgacga cgctgatgct ggcgggaatt cgggagatcc tcgtcatcac cacgccgcag
      361 gatcagggcg ctttccgcca cctgctgggc gatggcagcc agtggggcgt ggagatccgg
      421 tatgccgtgc agccagagcc cagggggctg gcgcaggcct acacgatcgg cgcggatttt
      481 gtggctggcg gcccgtcctg cctggtgctg ggcgacaata tcttctttgg ccatggcctg
      541 ccgcaggtgc tggcgggggc cagggcgggg ctggccgcgg ggggcgcccg ggtgttcgcc
      601 tatcaggtgg cagacccgga gcggtatggc gtggtggaat tcgatagcgc gcgccgggcc
      661 gtttccatcg aggagaagcc ggccgcgccc aaatcgaact gggcggtgac cgggctttac
      721 ttctacgatg ccgaggtggt ggagatcgcc cgttcgatcc agccgtcggc gcgcggcgag
      781 ctggagatca ccgatgtcaa ccgcgcctat ctggccgccg gccggctcag cgtggaagtg
      841 atgggccgcg gctatgcctg gctggatacc ggcacgcccg aaagcctgct ggaagcggcg
      901 caattcgtcg ccacgctgga aaagcggcaa ggcatgaaga tcgcctgccc ggaagagatt
      961 gcctggcgga tgggctatat cgacgatgcc ggcctcgaac aggccgccgc ccgccttggc
     1021 aagggcgatt atgccgccta tctgcgccgc ctgctgcaga tcgggcagct gggctgagct
     1081 ggtctttgct gcggcaagat gtgcatttgc gttgtgcagt tgcaatagcg ctgcaacagg
     1141 attggtgata agggtttttt cgtatctatt cgctgatccg ggtggaaatg cctgcgttat
     1201 gctgtcaaac atcagccggc gcagacaaaa ccacgctact gacgagataa aagggactta
     1261 tgccgaagat tacgcccgtg attttgtgcg gtggcagcgg cacgcgtttg tggccgcgca
     1321 gccgcaaggc caagcccaag cctttcctgc cgctggtggg cgattatacg ctgtttgagg
     1381 aggccctgcg ccgcacggcc gaccgcgcga ttttcacagc gccggtgatc gtcaccggca
     1441 ccgcccatct ggaacatgtg gaaagccagc tggctttcgc ccccgatgcg caggtgatcg
     1501 tggagccggc cgcgcgcaac acggccgcgg ccatcgccct tgccgccctg cgcctgcccg
     1561 aagatgcggt gatgctggtg tgcccgagtg accatcacat tggcgatccg gccgctttcg
     1621 tggccgctgc gcaggccgcc gccggccttg ccgcggaagg ctggctggtg gcctttggca
     1681 tcaccgccac ggcgccggaa accggctttg gctatgtgaa gcgcggcgag gcgattggcg
     1741 cggccggttt ccgggtggat cgcttcgttg aaaagcccga tctggagcgc gccatcacct
     1801 tcctggccga gggcagctac agctggaatg gcggcatctt cgccttcagc gccggcactt
     1861 tcctggccga actggccgcc caccgcccgg ccatcgccga gcaggcgcgc aaggccgtgg
     1921 ccgccggcca tgaagagggc cgccgcttcc acccggatgc ggcgctcttt gccggggtgg
     1981 aaagcgattc gatcgattac gcggtgatgg agaagaccag ccgcgccgcc atggtgcccg
     2041 ccgccatggc gtggagcgac attggcaact ggcaggcgct gcatgccgcc cgcctgcagg
     2101 atgatcgcgg caatgccgtg ctgggcgcgg tagagctggt cgattgcagc aatgtgctgg
     2161 tggaaagcga tgggccgcgc gtgtcggtga tcggccttga gaatgtgatg gtggtcgtcg
     2221 atggcggcga ggtgctggtg acgacggcgg ccggggccca gaaggtgggc aagctggccg
     2281 gggccgccaa ccagtgaaca tggcggcggc catctcgccc gcgctgccgg tgcggctggt
     2341 ggaaaagccc tggggggtgg aggtattgcc cgcccccttt gccggcgatc cacgcggggc
     2401 tggccggcgc attggcgaga tctggttcga gccgccggcc gctgtgcccg aactgttgct
     2461 gaaatatatc ttcagcagcg acaagctctc ggtgcaggtg cacccttccg atgcgcagac
     2521 cctggcgcag gggctgggcc ggcagggcaa ggaggaatgc tggctggtga tcgatgccgg
     2581 gccggatgcg cggctgggca ttggctttca cgagccggtg gagccagagg cgatgcgcgc
     2641 ggcggccctt gatggcagca tcgagcagat gctggtgtgg caccaggtac aggccggcga
     2701 tttcttctac attcccgcca acaccgtgca tgccattggc gccggggtca gcgtggtgga
     2761 agtgcagcag aacagcgata tcacctatcg cctgttcgat tatggccgcc cgcgcgcgct
     2821 gcatctggaa gagagcctgg ccgtggcgct gggcacgccc tatgatctgg cgcgccatca
     2881 tcggcggctg gggccgggcg gcgatgcgca gctggtggaa gggccctatt tccggctgga
     2941 ccgggtggaa ggcctgcccg atgcgggcct gcgcgcgggg ctggcggcgc gctatggcca
     3001 tgcgccgctg gtggtgctgc cgctggccgg cgcgctgacg gtgatgggcg ccacggtggc
     3061 cgctggcggc tgcgcgattg cctccacact ggcggatatt gcctttgcgc ccggggggca
     3121 gtgcctcctc gcccaacccc taggggtttc agcctaactc tcttgcgcgg atcgccgctg
     3181 caccggcccg gcgggcgctt gccccgccgt tgtggagggg ctttgccttg agaggggcgg
     3241 ggccttgtgg tgccgtgcct tatggccgat cggcaatggt gccgcggcca cagggcaagg
     3301 tggctgatga gcgaggcgcg gcatctgttt gatttggcgc ggcataaggc aatgcctgcc
     3361 gcgcgcgcgc cggatggggc cgcgctgctg gcgcgcggcc gcgcggtgat cgcggcggaa
     3421 gggcaggggc tggcgctgct ggccgccggg ctggatggtc gctttgccga tgcggtggcg
     3481 ctgattgcgg cggggcggcg gctggtgctg gcgggcatgg gcaaatcggg ccatatcgcg
     3541 cgcaagctgg cggccacctt tgccgccacc ggcacgccgg cgctgtttgt gcacccggca
     3601 gaggccgcgc atggcgatct gggcatgatt gctgcgggcg atgtgctgct gctgctgtcc
     3661 aattcgggcg aaacgcgcga attgcggccg ctgctggcgc atggccgggc gctgggctgc
     3721 aaggtgatcg gcatggccgg gcgggcgggc tcgttcctga tggcggcggc cgatgtgggg
     3781 ctgtgcctgc cgcaggtggc ggaggcttgc gcgatcagct gcgcgcccac cgtgtcgagc
     3841 acgatgcaga tggcgctggg cgatgcgctg gcgctggcgg tgatggacct gtgcggcgtg
     3901 gcgccggcgc ggctgcgggc gctgcatccg ggcggcgcgc tggggctgca gctggcccgg
     3961 gtggacgagg tgatgcaggg cccgggcgcc ctgccgctgg tggaggaagc cacgccgatg
     4021 gccgaggtga tctcggtgat cacgcgcggg cgctttggcc ttgccggggt gacggatggc
     4081 gcggggcggc tggtgggggc gatcagcgat ggcgatctgc gccgccatat cgggcagctg
     4141 gcaggcgctg tggcgggcgc tgtgatgagc cgggcgccgg tgtgggtgcc ggccggcatg
     4201 ctggcgcgcg aggcgctggc gctgatgggc caggcgcgga tctcggcggt gttcgtgatg
     4261 gcagaggggg caggcggcgc cggccggccg ctgggcatgc tgcatgtgca tgatctgctg
     4321 cggctggggg cggcatagag gatggccctg ctgcgcgata cagaggcttg gccccttggc
     4381 aaggccagcg tgccgggcgg ggcctgtgcg gtggtgatcc cggcgcgtta tggctccacg
     4441 cgttatcccg gcaagccgct ggcgctgctg cgcgggggca gcggcgaggc caggccgttg
     4501 atccgccgca gctgggaagc ggcgcgggcc gggctggcgg agatgggcat gcctgcgcgc
     4561 ctgctggtgg caacggacga cccgcgcatt gccgaagccg tcgccgcttt tggcggcgag
     4621 gcggtgttga ccccgcccga ttgccgcaac ggcacggagc gctgcgcggc cctgctggag
     4681 gctttgccgg catcggtgga ggtggtggtg aactggcagg gcgatgcgcc cttgatgccg
     4741 ccggccgcgc tggcggcggt ggtggccggg ctgggccggg atgcgggcgc ggtgatggca
     4801 accgcggccc tcgcctgcag cgcgcaggtc catgcccatc tgctggccga tgcggcggcg
     4861 ggccgggtgg gcggcaccac ggtggtggtc aacgggcggg gccgggcgct ctatttttcc
     4921 aagcgggtgc tgccttttgc gccggcggcc gggcatgcgg cggtgcggct gcatctgggg
     4981 ctctatgcct atcgccgcgc ggcgctggca gcctatgcgg cgctgccgcc cagccggctg
     5041 gagcaggaag aggggctgga gcaattgcgc tttttcgagg gtgagggggg cattgcggtg
     5101 gtcgatctgg cagagccggg ctggccggtg atcgagctga acaaccccga ggatgcgccg
     5161 ctgatcgagg cggtgctggc acagcggggg atcgcatgag cgcggtggcg ctggcgccgg
     5221 gcctcacctt cggcaacgac cggcccttcg tgctgatcgg cggcatgaat gtgatcgagg
     5281 ggcgcgacag cctgctgcgg gtggccgaac atttcgcccg cacctgcgcc gatctggcca
     5341 tcccgctggt gttcaaggcc agctgggaca aggcgaaccg ctcggccatc acctcgttcc
     5401 gcgggccggg gctggaggaa gggctggcgc tgctggccga ggtgaaggcg gctttcggcc
     5461 tgccgatctg caccgatgtg catgagccct ggcaggcagg ccccgtggcc gaggtggccg
     5521 atatcgtgca gctgcccgcc ttccttgccc ggcagacgga tctggcgctg gcgctggcgg
     5581 gcaccgggcg ggtggtgctg gtgaagaagc cgcaattcct cagccccgcc gaaatgccgc
     5641 atattctggc caagctggcg caaggcggcg cgcctggcgc cctgctgggg gagcggggga
     5701 ccagctttgg ctacaacaat ctggtggtgg acatgctggg catggatctg atgaagccga
     5761 tggcgccggt ggtgttcgat gtgacccacg ccctgcagcg ccccggcggc cggccggaca
     5821 gcgccgatgg caggggctcg caggctgccg cgctggcgcg ggcggggctg gcggtgggca
     5881 ttgccggcct gtttgtggaa gcgcatcccg atccggcggc ggccctgtgt gatggcccct
     5941 cggccctgcc gctgggggat ctggcgccgt ttctgcggca gatgcaggcg gtggaccagg
     6001 ccgtgaaaca ggcggtgaaa caggcggttt aggccatggc cggcgcgccg ccctttctgc
     6061 gcatgccgcc ttttcccggc gcgcgggtgc gccagctggc atggcgccgc ccccggcccc
     6121 tgccgctgga cgaagccgcg ctggctgagg ccgccgccct tgccgctgcc ctgcgcgagg
     6181 agcaggtggg cggcgatttc tggggcgcgc ggccggtggg ggtggctgcg gccagcggcg
     6241 gggtggtgcg ggcgatgccg cgcggggctg atccgtggca tgtggcgggc gcggcct
//
LOCUS       DASRYE010000312        12605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_171228,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000312 DASRYE010000000
VERSION     DASRYE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171228"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFF98_16055"
     CDS             complement(<1..1012)
                     /locus_tag="VFF98_16055"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZU65202.1"
                     /translation="MDNLKQMLAPQDRRGFFKAAGVGAAGVYLFGLAACKGPGGADRV
                     IKLGLVTPTTGPLAPFASADDYVINQLKPLLDKGITLGGKTHAVQVVLRDSQSNPKRA
                     AEVAQDLIDREKVDLLLAAGTADTTNPTADQAESAGVPCITTDAPWEAFFFGRHGDPA
                     KPFGWTYHFFWGLEQIASVYAGMWQGAATNKTIGALWSNDPDGTAIRKGVTEKIARVG
                     GFRLVDAGLFAPGSNDFSAQISQFKAAGVEIVTGLFIPPDFATFWTQCAQQGFHPKVV
                     TVAKALLFTQVVAGLGPRGHGLSSEVWWSASHPFKSSLSGVTAGQICADYVARSGQPW
                     SPP"
     gene            complement(1145..2023)
                     /locus_tag="VFF98_16060"
     CDS             complement(1145..2023)
                     /locus_tag="VFF98_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZU65203.1"
                     /translation="MATETGGSDYPQGPEGFTAGALPPPGTYFLNYVEHYTAGRFNDG
                     NGHAGFFPDFQVAATADVLRVVQVDKITVLGASVGHAVILPVVDLSVGIGTAHDRRSG
                     IGDMTLTPLILGWHGKSLHAVAALDIYAPTGAYSQNRLANIGRNAWAAEPVLALAYYP
                     KGGFTVDVKLMYDFNGTNRQGNINPLNPTGADYRSGQEFHADFAVGQQVGKVQLGVSG
                     YVYRQTTGDKVANAAAQATLDALGGLKGKAFALGPSAHLNLGKVMLIGTYQHEFQADY
                     RPQGDKFWVKAVIPLR"
     gene            complement(2208..3134)
                     /locus_tag="VFF98_16065"
     CDS             complement(2208..3134)
                     /locus_tag="VFF98_16065"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HZU65204.1"
                     /translation="MRKGVIRPGHVQLRVLDLEPAVKHYTELVGLIETHRDAMGRVYL
                     KGWSDTELFSVVLREADEAGMDCQGFKVRDDATLSALGRDLAAWGTQVEEIAPGELDH
                     CGRRLRFLSPSGHTLELYADKAYTGRWGISRVEPEAWPRGLRGMRVQRFDHCQLHGRD
                     IEATRDLLSEVLGFYVTEHVLDQEGGRNLAIFMSLAMKAHDIALILDERHGTFHHAAF
                     HVESWESLLRAGDLIAMTGTSLDTAPTRHGITHGQTTYFFDPSGNRNEVFAGGDVHYP
                     DQPPSVWYEKNLGKAIFYHHQKLSEKFMTALT"
     gene            complement(3160..3618)
                     /locus_tag="VFF98_16070"
     CDS             complement(3160..3618)
                     /locus_tag="VFF98_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HZU65205.1"
                     /translation="MRQDWEETLEMGAFAVAPSGLGQGMARAGQHRSISCRRDLEGRR
                     ELEAAQAGPCAPALATDQATGRAVLVGPSETLLQAIERDNPRSATVGCRNGGCGVCRV
                     QLLSGKVRLGKMSRRFVTVDMERERFALACRVYPQGDLTFRLAPLDPRAL"
     gene            complement(3660..3851)
                     /locus_tag="VFF98_16075"
     CDS             complement(3660..3851)
                     /locus_tag="VFF98_16075"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HZU65206.1"
                     /translation="MPIVQVNLIQGRSEEQKSAMIREVAHAISQTLDAPIDTVRVMIN
                     ELPSSNWGMGPVTAKSKGR"
     gene            complement(3863..4648)
                     /locus_tag="VFF98_16080"
     CDS             complement(3863..4648)
                     /locus_tag="VFF98_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65207.1"
                     /translation="MALAQETVNLLAERLETARRDRAGIVKITNDHPDLSWADAYVIQ
                     DRLRDLQQDLGVAGLKMGFTSRAKMAQMGCKDPINGYLTEAGRIAPGGSVSRGAYIFP
                     RVEAEIAIVTSRELKGPGCTIAQVQAAVAQVLPALEVIDSRYLNYGFDLPSVIADNTS
                     AAAFVVGDRGVPVAGLDLKAMGVVTLRNGEVVNTAAGAAVLGSPLNSVALLANMLGRR
                     GLSIPAGTLILTGGITEAVPINPGDHFTIKFQNLGQIDLSVTE"
     gene            complement(4661..5287)
                     /locus_tag="VFF98_16085"
     CDS             complement(4661..5287)
                     /locus_tag="VFF98_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65208.1"
                     /translation="MADISNSAENLTVARIGRRVLGIGQAARAQLGLSQPAFGAITAD
                     MLVDDTDAVPMAGGDAPLAVATLAFLLERDLAGGEVDIADVLEATALVFPAIEVRDPQ
                     AAAHGFGAHVVLGDGGLAPAAIDLECCGVVAELNGALAATGAGAAALGHPAKAVAWLA
                     NALDAQGDPLRAGEIVLAGELTAPVAVKAGDALRVTVGGLGNAGLRFE"
     gene            5410..6315
                     /locus_tag="VFF98_16090"
     CDS             5410..6315
                     /locus_tag="VFF98_16090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU65209.1"
                     /translation="MDIRQLENFLAVCEEMNVGRAAGRVHLSQPALSRQIQALEAEVG
                     VALFDRSSKSIRLTAAGHHYERELRVVLNSLRNSARTARAIAAGHQGEIKVGIFGSAI
                     LDFIPATLERFGQLHPQARVSLASMDKDMQIDGLRRRTLDLGFNRLVPEEPDISVELV
                     RTEPLVLAMRSDNPLASEAQIHLEHIVETPLVLYPTHVRNSLITRVHQLFADIGAAPL
                     VAHEVADLISAMSLISAGLGVGIVPIAATRITLRDVVFRPFAGKPPTVNLACLYRKHD
                     DNPLLLRLLDCVRQMGREQEAPISS"
     gene            complement(6307..7092)
                     /locus_tag="VFF98_16095"
     CDS             complement(6307..7092)
                     /locus_tag="VFF98_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU65210.1"
                     /translation="MRNQKPLVAATQRSLTMLEAIVADRAASSVSALARAAGLPVATA
                     HRQVATLVAEGFLAPAGHGRHIAGPRLRALGALVEDVQLIANIAAPLLRKLAARFGGV
                     AQLGTFENDMVTYRVKTGAAANTLFTRVDMQMEAYCSGVGKVLLAHLPAAQRSAYLAG
                     GPFVALTPATITAPADLARELEEVARQGFAQDRGEIAPDLFCLAVPIRDRAGAVPAAL
                     SLSRRGAAAPAPDPRALHALQAVARAVEETCFGRGPDRASGSG"
     gene            7182..7964
                     /locus_tag="VFF98_16100"
     CDS             7182..7964
                     /locus_tag="VFF98_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65211.1"
                     /translation="MTDKHEAAAILLRNAYAGGAVPPLRAYLAPVDIAGAYAVQEINT
                     RFWQAQGRRIVGRKAGLTARAVQAQLGVDQPDFGVLFDDMAVADGGFLDPARCLQPKA
                     EAEIAFVLGADLPSADTTPEAVAAAVGTVHAAIEIVDSRIADWQITFSDTVADNGSSA
                     FFTLAGEGKALDGLDLYTAGMVMEVNGQIVSLGAGAAALGHPLNAAAWLARTLAARGD
                     PLRKGDILLAGALGPMVALQAGDHVVARVGGLGSCSFTYAKG"
     gene            7969..8922
                     /locus_tag="VFF98_16105"
     CDS             7969..8922
                     /locus_tag="VFF98_16105"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HZU65212.1"
                     /translation="MAKTKVAIIGSGNIGTDLMIKIMRLSKVLEMGVMVGIDPASDGL
                     ARAARMGAATTHEGIDGLVNMPEFADIGIVFDATSAGAHKRNNEILQAHGKRVIDLTP
                     AAIGPYTIPPVNGEANLDAPNVNMVTCGGQATIPIVAAVNRVARVHYGEIVASISSKS
                     AGPGTRANIDEFTETTSEAIVKVGGATRGKAIIILNPAEPPLIMRDTVFCLCEEADPE
                     AIRQSIDDMVADVQSYVPGYRLKQQVQFEHIGSNKPLRIPEMASTGQTEFTGLKVSVF
                     LEVEGAAHYLPAYAGNLDIMTSAALKTAEKIAIRHFAGEHA"
     gene            8919..9944
                     /gene="dmpG"
                     /locus_tag="VFF98_16110"
     CDS             8919..9944
                     /gene="dmpG"
                     /locus_tag="VFF98_16110"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZU65213.1"
                     /translation="MTFDPKTQKLYIQDVTLRDGMHAIRHQYGLDHVQAIARALDRAK
                     VDAIEVAHGDGLQGSSFNYGFGAYTDWDWIGAVAEVLEHSVLTTLLLPGIGTVHDLKH
                     AYEMGVRSVRIATHCTEADVSRQHIEAARALGMDVSGFLMMSHMTSPEALASQALLME
                     SYGAHCVYVTDSGGAMNMDDYAARLQAYDRVLKPETQRGVHAHHNLSLGVANSLVAVQ
                     NGAVRVDASLAGMGAGAGNAPLEVFIAAADRQGWNHGCDLYALMDAADELVRPLQDRP
                     VQVDRETLTLGYAGVYSSFLRHAEKAAGDYGVDTRSILAEVGRRKMVGGQEDMIIDIA
                     LDLAGAR"
     gene            10034..10627
                     /locus_tag="VFF98_16115"
     CDS             10034..10627
                     /locus_tag="VFF98_16115"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU65214.1"
                     /translation="MPRIVDHEQRRKDIARVVSALVLKGGAEAVTIRAVAQACGFSTA
                     VVCHYFASKHAMLAYTQRLAWQRGLARITAAGAAGAPLEACLAHALPDTPAGWEDWHC
                     WMAFWGQAPETDGIEADWRESIAVSNAVFVDLVRRAQAQGAFDPEEDPSEAATGIQIM
                     LNGIASLVAQGRGEWPAERQRAVLRRQLRRLGYRPAE"
     gene            complement(10609..11457)
                     /locus_tag="VFF98_16120"
     CDS             complement(10609..11457)
                     /locus_tag="VFF98_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZU65215.1"
                     /translation="MKLGLHQITANECDPLTLVDLAAEVGCEEVCLFTHIPRAALPGD
                     AAPTLFPVVTRPLVARLRERLDAAGVGVGNVEFFPVTAAGDAGLYRESFEIAAAIGAR
                     RAVTHIHDAEDARAVDSLGRLGALAAEHGLRLGLEFMGLTPACASIERAAWFVEQVGV
                     AQVGIGVDALHLVRTGGRPADVARIPSALFTYAQICDAAGLQATPDYLHEALERRLPG
                     DGDFPLAAFVAALPEGVAVDVEVPSAARIAAGQPALGHARTAVARARRLLTGIGAARK
                     DHSAGR"
     gene            complement(11469..11942)
                     /locus_tag="VFF98_16125"
     CDS             complement(11469..11942)
                     /locus_tag="VFF98_16125"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU65216.1"
                     /translation="MLDYTRAFHTGVLTPDLDAAIAFYARTLGLRFAEPVVLEALALW
                     TPERGRHTVRNRFTYSLDGPLHLELQGGTPGSFYDPALSRGDHLGIWVDDLPATVARL
                     GGAGWQVIAAGAAPEDGYGLFTYLQPAAGGMVIEIVSQALQPAFARWWAGGPLAV"
     gene            complement(11966..>12605)
                     /locus_tag="VFF98_16130"
     CDS             complement(11966..>12605)
                     /locus_tag="VFF98_16130"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HZU65217.1"
                     /translation="HYIGFIRRRAGMSHADFARYWGTVHRDLVMAFADPGPILGYVQD
                     VGLAAAPAGLANSFDGAPELWIRDAAALAELFAAEGFRHAYDVDSPRFITLPAISFFV
                     ADTVIRDGAADGVKLMRLIPADPGRPALQAAWAGAHQPIGMAGAEPCRLVRARILSGE
                     AEGSDAAAWLGIESSWWPDAETAARAWQARDAALTGAERVLLARERVILGPC"
BASE COUNT         2107 a         4408 c         4117 g         1973 t
ORIGIN      
        1 gcggcgggct ccacggctgg cccgatcggg caacataatc ggcgcagatc tggcccgccg
       61 tcacgcccga gaggctggat ttgaacggat ggctggccga ccaccacacc tcgctggaga
      121 ggccatggcc gcgcgggcca agccctgcca ccacctgcgt gaagagcagc gccttggcca
      181 ccgtcaccac cttggggtgg aagccctgct gcgcgcattg cgtccagaag gtggcgaaat
      241 cgggggggat gaacaggccg gtgacgatct ccaccccggc ggccttgaac tggctgatct
      301 gggcggaaaa atcgttcgat ccgggggcaa agaggccggc atcgaccagc ctgaagccgc
      361 ctaccctggc gatcttttcg gtaaccccct tgcggatcgc agtgccatcc gggtcgttcg
      421 accacagcgc gccgatggtc ttgttggtgg ccgcgccctg ccacatgccg gcatagaccg
      481 aggcgatctg ttccagcccc cagaagaagt ggtaggtcca gccgaaaggt ttggccggat
      541 caccgtggcg gccgaagaag aaggcttccc acggcgcatc ggtagtgatg cagggcacgc
      601 ctgcgctttc ggcctggtcg gccgtggggt tggtggtgtc ggcggtgccg gcggccagca
      661 gcagatcaac cttttcccgg tcgatgagat cctgcgccac ttctgcggcg cgcttggggt
      721 tcgactggct gtcgcgcagc accacctgca cggcatgggt cttgccgccc agcgtaatgc
      781 ccttgtcgag cagcggcttg agctggttga tcacatagtc atcggcgctg gcaaaggggg
      841 ccagcgggcc ggtagtgggc gttaccaggc ccagcttgat gaccctgtcc gccccgcccg
      901 gccccttgca ggccgcaagg ccgaacagat agacgccggc cgcgcccacg ccagccgcct
      961 tgaagaaacc gcggcgatcc tggggcgcca gcatttgctt gagattgtcc atcgtctctc
     1021 tcccccgcat tgggagcatc acgccggcag ggttctgcgc atcggcgcga tgcggcacat
     1081 cgaaaggcca gggcgcggga tatgggtgtg cagacatccc tgcgccctgg ccggcctgtt
     1141 cggatcaacg cagcgggatc accgccttca cccagaactt gtcaccctgc ggccggtaat
     1201 cggcctggaa ttcatgctga taggtgccga tcagcatgac cttgccgaga ttgagatggg
     1261 ccgaggggcc aagcgcgaag gccttgccct tcaggccgcc cagcgcatca agcgtggcct
     1321 gcgcggcggc attggcaacc ttgtcgcccg tggtctggcg atagacatag cccgagacgc
     1381 ccagctggac cttgcccacc tgttggccca ccgcgaaatc ggcgtggaat tcctggcccg
     1441 accggtaatc ggcgccggtg gggttgagcg ggttgatgtt gccctgccgg ttggtgccgt
     1501 tgaaatcata catcagcttc acgtccaccg tgaaaccgcc cttgggataa taggccagcg
     1561 ccagcaccgg ctcggcggcc caggcgttgc ggccgatgtt ggccaggcgg ttctggctgt
     1621 aggcgccggt gggcgcatag atgtcgagcg cggccacggc atgcaggctc ttgccatgcc
     1681 agcccagaat cagcggggtc agcgtcatgt cgccaatgcc gctgcgccgg tcatgcgcgg
     1741 tgccgatccc caccgacaga tcaacgaccg gcaggatcac cgcatggccc accgaggcgc
     1801 ccagcacggt gatcttgtcc acctgcacca cgcgcagcac atcggcggtg gcggccacct
     1861 ggaaatcggg gaaaaagcct gcatggccat tgccatcgtt gaagcggccg gcggtgtaat
     1921 gctctacata attgaggaaa taggtgcccg gcgggggcag ggcgccggca gtgaagcctt
     1981 cggggccctg tggataatcg ctgccgccgg tttcggtggc catggccggg gccgcacatc
     2041 ccagcgcccc tgccacacac gaaagcgccc atcgcctcgt ggctgtcctg aatgttgtca
     2101 tggtcctctc cctgttgaat tctcccttgc cgatcgcggg cacaacccgc cccttcccga
     2161 aggcgcgggc cttcgggtgg tggtctttgc gttgtgccgg gatcaggtca ggtcagtgcc
     2221 gtcatgaatt tttcggaaag cttctggtgg tgatagaaaa tcgccttgcc cagattcttc
     2281 tcgtaccaga cgctgggcgg ctgatcggga taatgcacat cgcccccggc aaacacttcg
     2341 ttgcggttgc ccgaggggtc gaagaaatag gtggtctgcc cgtgggtgat gccgtggcgg
     2401 gtgggggcgg tgtcgagcga ggtgccggtc atcgcgatga gatcgcctgc ccgcagcagg
     2461 ctctcccatg actcgacatg gaaagcggca tggtggaacg tgccgtggcg ctcgtcgagg
     2521 atcagcgcga tatcatgcgc cttcatcgcc agcgacatga agatcgccag attgcggccc
     2581 ccttcctggt cgagcacatg ctcggtgaca taaaagccca gcacctcgct cagcaggtcg
     2641 cgcgtcgcct cgatgtcgcg gccatgcagc tggcaatgat cgaaccgctg cacccgcatt
     2701 ccgcgcaggc cccgcggcca ggcttccggc tccacccgcg agatgcccca gcgcccggtg
     2761 tatgccttgt cggcatagag ttcgagcgtg tggcccgagg gcgagaggaa gcgcaagcgg
     2821 cgcccgcaat ggtccagttc ccccggcgcg atctcctcca cctgggtgcc ccaggcggcc
     2881 agatcgcggc ccagcgcgct cagcgtggca tcgtcgcgca ccttgaagcc ctggcaatcc
     2941 atgcccgcct catcggcctc gcgcagcacc accgaaaaca gctcggtatc gctccacccc
     3001 ttgagataga cgcggcccat ggcatcgcga tgcgtctcga tcaggcccac cagttctgtg
     3061 taatgcttca ccgccggctc cagatcgagc acgcgaagct ggacatgtcc ggggcggata
     3121 actcccttgc gcattgtttt atcctctctt tcaggctggt cagagcgccc gcgggtccag
     3181 cggggccagt cggaatgtca gatccccctg tggatagacg cggcaggcca gcgcgaaacg
     3241 ctcgcgctcc atgtccaccg tcacgaagcg gcggctcatc ttgcccaggc gcaccttgcc
     3301 gctcagcagc tgcacccggc acacgccgca tcccccgttg cggcagccca cggttgccga
     3361 acgcggattg tcgcgctcga tcgcctgcag cagggtttcc gaaggcccga ccagcaccgc
     3421 ccggcccgtg gcctgatcag tggccagcgc cggcgcgcaa gggcccgcct gggcggcctc
     3481 aagctctcgc cggccctcaa ggtcgcgccg gcaagatatg ctgcggtgct gcccggcgcg
     3541 ggccatgcct tgccccagcc cggatggagc gacggcaaag gcccccatct ccagcgtctc
     3601 ttcccaatcc tgcctcatcg ctcatcctct cttgcccgcc cccgttctgt gccggggtat
     3661 cagcggccct tggatttggc cgttaccggc cccatgcccc aattgctgct gggcaattca
     3721 ttgatcatca cccgcaccgt gtcgatgggg gcatccagtg tctggctgat ggcatgggcc
     3781 acttcgcgga tcatggcgct tttctgttct tcgctgcgcc cctggatcag gttgacttgc
     3841 acaatcggca ttgccttggc ccttactctg tcacgctgag gtcgatctgc cccagattct
     3901 ggaacttgat ggtgaaatgg tcgcccggat tgatcggcac cgcctcggtg atgccgccgg
     3961 tcaggatcag cgtgcccgcc gggatggaca ggccacgccg ccccagcatg ttggccagca
     4021 aggccaccga attgagcggg ctgcccagca ccgcagcgcc cgccgccgtg ttgaccacct
     4081 cgccattacg cagcgtgacc acgcccatcg ccttgagatc cagccccgcc accggcacgc
     4141 cccgatcccc caccacgaaa gcggcggccg atgtgttgtc ggcaatcacc gagggcaggt
     4201 cgaagccata gttgagatag cggctgtcga tcacctccag cgcgggcaga acctgcgcca
     4261 cggcggcctg aacctgcgcg atggtgcagc ccggcccctt cagctcgcgg ctggtgacga
     4321 tggcgatctc ggcctccacg cgggggaaga tataagcccc gcgcgacacg ctgccgcccg
     4381 gcgcaatccg gcccgcttcg gtgagatagc cgttgatcgg atccttgcag cccatctgcg
     4441 ccatcttggc ccggctggtg aagcccatct tcagcccggc gacgcccaga tcctgctgca
     4501 aatcgcgcag ccggtcctga atgacatagg catcggccca gctgaggtcg gggtggtcgt
     4561 tggtgatctt cacgatcccc gccctgtcgc gccgcgccgt ctccagccgc tcggccagca
     4621 ggttcaccgt ttcttgtgca agtgccatca cagcgccacc ctattcaaac cgcagcccgg
     4681 cattgcccag gccgcccacc gtgacgcgca gcgcatcacc cgccttcacc gccaccggcg
     4741 cggtcaactc ccccgccagc acgatctcgc ccgcccgcag cggatctccc tgggcatcaa
     4801 gcgcattggc cagccatgcc accgccttgg ccggatggcc cagcgccgca gcgccggcgc
     4861 cagtggccgc cagcgcgccg ttcagctcgg ccaccacgcc gcaacattcc agatcgatgg
     4921 ccgcaggcgc cagcccgcca tcgcccagca ccacatgggc gccaaagcca tgggccgccg
     4981 cctgcggatc cctgacctcg atggcgggga agaccagcgc cgtggcttcc agcacgtcgg
     5041 caatgtccac ctcgccaccg gccagatccc gctccagcag gaaggccagc gtggccaccg
     5101 ccagcggcgc atcgcccccg gccatgggca ccgcatcggt gtcatccacc agcatgtcgg
     5161 cggtgatcgc gccaaaggct ggctggctca ggcccagctg cgcgcgcgcc gcctggccaa
     5221 tgcccaatac ccggcgaccg atacgcgcga cggtgagatt ttcggcagag ttcgaaatgt
     5281 cggccatgtc cctctatccc cagccacctg tgagcggccg ttttgcttgg cacaggattt
     5341 accccggcat aaacatgtga tccaatgccg attgggcacg atatcatgca gcaggcgcat
     5401 gggtgagcga tggacatccg acaactcgaa aattttctgg ccgtatgcga agagatgaat
     5461 gtgggccgcg cggccgggcg cgtgcatctc tcacagcccg cgctgagccg gcagatccag
     5521 gcgctggagg ccgaggtcgg cgtggcgctg ttcgaccgca gctccaaatc catccggctg
     5581 accgccgccg ggcaccatta cgagcgggag ctgcgggtgg tgctcaacag cctgcgcaac
     5641 agcgcccgca ccgcgcgcgc aatcgccgcc ggccaccagg gcgagatcaa ggtcggcatt
     5701 ttcggctcgg cgatcctcga tttcatcccc gccacgctgg agcgcttcgg ccagctgcat
     5761 ccacaggcgc gggtaagcct ggcctcgatg gacaaggaca tgcagatcga tgggctgcgc
     5821 cggcgcacgc tcgatctggg cttcaaccgc ctggtgcccg aagagcccga catcagcgtc
     5881 gaactggtgc gcacagagcc cttggtgctg gccatgcgca gcgacaatcc gctggccagc
     5941 gaagcgcaga tccacctgga acatatcgtc gagacgccgc tggtgctcta tcccacccat
     6001 gtgcgcaaca gcctgatcac ccgcgtgcac caattgtttg ccgatatcgg cgcggcgccg
     6061 ctggtggcgc atgaagtggc cgatctgatc agcgccatgt cgctgatatc ggcagggctc
     6121 ggcgtgggca tcgtgcccat cgccgccacg cggatcacgc tgcgcgatgt ggtgttccgg
     6181 cctttcgcgg gcaagccgcc cacggtcaac ctggcctgcc tctatcgcaa gcacgacgac
     6241 aatccgctgc tgctgcggct gctcgattgt gtgcggcaga tgggcaggga gcaggaagcg
     6301 ccgatctcat cctgagccgc tcgcccggtc tggcccgcgc ccgaagcagg tctcctccac
     6361 cgcccgggcc acggcctgca aggcgtgtag cgcgcgtgga tcgggcgccg gcgccgccgc
     6421 cccccgccgc gacagcgaca gcgccgccgg aacagcgccg gcccggtctc gaatcggcac
     6481 cgccaggcag aacaggtctg gcgcgatctc gccgcgatcc tgcgcaaagc cctggcgggc
     6541 cacctcctcc agttcgcggg ccagatccgc aggcgcggtg atggtggccg gtgtcaacgc
     6601 cacaaagggg ccacccgcca gataggcgct gcgctgggcc gccggcaaat gggccagaag
     6661 caccttgccc acgcccgaac aataggcctc catctgcatg tccacgcgcg tgaacaaggt
     6721 gttggccgcg gcacctgtct tgacgcgata ggtcaccata tcgttctcga aagtgcccag
     6781 ttgcgccacg ccgccaaaac gcgccgccag cttgcgcaac aggggcgcgg caatattggc
     6841 gatcagctgc acatcctcca ccagcgcccc cagcgcccgc agccgcgggc cggcgatgtg
     6901 ccgcccatgc ccggccggcg ccagaaaccc ctcggctacc aatgtcgcca cctggcgatg
     6961 cgcggtggcc accggcaggc ctgccgcgcg ggccagcgcc gacacgctgc tggccgcgcg
     7021 atcggccacg atcgcctcca gcatcgtcag gctgcgctgt gtggcggcga ccaggggctt
     7081 ttgatttctc atctggcgag aaattagcag gcggctcgtc tggcgccaag ccccgccaga
     7141 tcctaatcat gcgccatccc cgctggagag gacatgccag gatgaccgac aaacacgaag
     7201 ccgcagcgat cctgctgcgc aacgcctatg cgggcggcgc ggtgcccccc ttgcgcgctt
     7261 atctcgcccc ggtagacatc gccggcgcct atgccgtgca ggaaatcaac acccgcttct
     7321 ggcaggcgca gggccgccgg atcgtgggcc gcaaggccgg gctgacggcc agggccgtgc
     7381 aggcgcagct gggcgtggac cagcccgatt tcggcgtgct gttcgatgac atggcagtgg
     7441 ccgatggcgg cttcctcgat ccggcccgct gcctccagcc caaggccgag gccgagatcg
     7501 cctttgtgct gggcgccgac ctgccctcgg ccgacaccac gcccgaagcg gtcgccgcgg
     7561 cggttggcac ggtgcatgcc gctatcgaga tcgtcgattc gcgcattgcc gactggcaga
     7621 tcaccttctc cgacacggtg gccgacaatg gctcctcggc ctttttcacg ctggcgggcg
     7681 aaggcaaggc gctcgatggc ctggatctct acaccgccgg catggtgatg gaagtgaacg
     7741 gccagatcgt ttcgctgggc gcgggcgcgg cagcgcttgg ccatccgctg aacgccgccg
     7801 cctggctggc ccgcacgctg gccgcgcgcg gcgaccccct gcgcaagggc gatatcctgc
     7861 tggcgggcgc gctggggccg atggtggccc ttcaggcggg cgatcacgtt gtggcccgcg
     7921 ttggcgggct gggttcctgc agtttcactt atgcgaaagg ctgagatcat ggccaagaca
     7981 aaggttgcca tcatcggttc gggcaatatc ggcaccgatc tgatgatcaa gatcatgcgc
     8041 ctgtccaagg tgctggagat gggcgtgatg gtgggcatcg acccggcctc cgacgggctg
     8101 gcgcgcgcgg cgcgcatggg tgctgccacc acgcatgaag gcatagacgg cctcgtcaac
     8161 atgcccgaat ttgccgatat cggcatcgtg ttcgatgcca ccagcgccgg cgcccacaag
     8221 cgcaacaacg agatcctgca ggcccatggc aagcgcgtga tcgacctcac ccccgccgcg
     8281 atcgggccct acacgatccc gccggtgaac ggcgaggcca acctcgatgc gcccaacgtc
     8341 aacatggtga catgcggcgg gcaggcgacc attcccatcg tcgcggcggt caaccgcgtg
     8401 gccagggtgc attatggcga gatcgtcgcc tcgatcagct cgaaatcggc agggcccggc
     8461 acccgcgcca acatcgacga gttcaccgaa accaccagcg aggcgatcgt caaggtgggc
     8521 ggcgccacgc gcggcaaggc gatcatcatc ctgaaccccg ccgagccgcc gctgatcatg
     8581 cgcgacacgg tgttctgcct gtgcgaggag gccgacccgg aagcgatccg ccagtcgatc
     8641 gacgacatgg tcgccgatgt gcaatcctat gtgccgggct atcggctgaa gcagcaggtg
     8701 cagttcgagc atatcggctc gaacaagccc ttgcgcattc ccgaaatggc cagcaccggc
     8761 cagaccgagt tcaccggcct caaggtttcg gtgttcctcg aggtggaggg cgcggcgcat
     8821 tacctgcccg cctatgccgg caatctcgac atcatgacct cggcggcgct gaaaaccgcc
     8881 gaaaaaatcg ccatccgcca ctttgcggga gagcacgcat gaccttcgac cccaagaccc
     8941 aaaagctcta catccaggat gtgacgctgc gcgatggcat gcatgccatc cgccaccagt
     9001 atggcctcga tcatgtgcag gcgatcgccc gcgcgctcga cagggccaag gtggacgcca
     9061 tcgaagtggc ccatggcgac gggctgcagg gctccagctt caactatggc tttggcgcct
     9121 acaccgactg ggactggatc ggcgctgtgg ccgaagtgct ggagcactcc gtgctgacca
     9181 cgctgctgct gcccggcatc ggcacggtgc atgatctgaa gcacgcctat gagatgggcg
     9241 tgcgcagcgt gcgcatcgcc acccattgca ccgaggcgga tgtcagccgc cagcatatcg
     9301 aggcggcgcg cgcgctgggc atggatgtct cgggctttct gatgatgagc catatgacca
     9361 gcccggaggc gctggccagc caggcgctgc tgatggaaag ctatggcgcc cattgcgtct
     9421 atgtgaccga cagcggcggc gccatgaaca tggatgatta tgccgcccgc ctgcaggcct
     9481 atgaccgcgt gctgaagccg gaaacacagc gcggcgtgca tgcccaccac aacctctcgc
     9541 tgggcgtggc caattcgctg gtggcggtgc agaacggcgc ggtgcgcgtg gatgcttcgc
     9601 tggcgggcat gggcgcgggc gcgggcaatg cgccgctgga agtgttcatc gccgcggccg
     9661 atcggcaggg ctggaaccat ggctgcgatc tttacgcgct gatggatgcc gccgatgagc
     9721 tggtgcgccc gctgcaggac cgcccggtgc aggtggaccg cgaaacgctg acgctgggct
     9781 atgccggggt ctattccagc ttcctgcgcc atgcggaaaa ggcggccggg gattatggcg
     9841 tggatacgcg cagcattctg gccgaagtgg gccgccgcaa gatggtgggc ggccaggaag
     9901 acatgatcat cgacatcgcg ctcgatctgg ccggcgcacg gtaaaccgca agggggctgg
     9961 ccgcttgccg gggccggccc ttgtgcgcag gagagcatag ccatcggccg ggcgatcggc
    10021 tagcgcagca ggcatgcccc ggatcgtcga tcatgaacag cgccgcaagg atatcgcccg
    10081 ggtcgtctcg gcgctggtgc tgaagggcgg ggcagaggcg gtcaccatcc gcgcagtggc
    10141 gcaggcctgc ggcttcagca cggcggtggt ctgccattat ttcgccagca agcacgccat
    10201 gctggcctat acccagcgct tggcctggca acgcggcctg gcgcggatca ccgcggcagg
    10261 ggccgccggc gcgccgcttg aggcgtgcct tgcccatgcc ctgcccgaca ccccggccgg
    10321 atgggaagac tggcattgct ggatggcctt ctgggggcaa gccccggaaa ccgacggcat
    10381 cgaggccgac tggcgcgaga gcatcgcggt gagcaatgcc gtgttcgtcg atctggtgcg
    10441 ccgcgcgcag gcccagggcg ccttcgatcc cgaggaggat cccagcgagg cggccaccgg
    10501 catccagatc atgctcaacg gcattgcctc gctggtggcg caagggcgcg gcgaatggcc
    10561 ggccgagcgc cagcgcgcgg tgttgcggcg gcagctgcgg cggctgggct atcgcccggc
    10621 ggagtgatcc ttgcgcgcag cgccaatccc cgtcaacagc cggcgcgccc gcgccactgc
    10681 cgtgcgtgca tggcccagcg ccggctggcc agcggcgatg cgcgccgccg agggcacttc
    10741 gacatcgacg gcaaccccct ccggcagcgc cgcgacaaac gctgccagcg gaaaatcgcc
    10801 atcgccgggc agccggcgtt caagcgcctc gtgcagatag tcgggcgtgg cctgcaggcc
    10861 ggcggcatcg cagatctggg cataggtgaa cagggccgac ggaatgcggg ccacatccgc
    10921 cgggcggcca cccgtgcgca ccagatgcag cgcatccacc ccgatgccga cctgcgccac
    10981 ccccacctgc tccacaaacc acgccgcccg ctcgatcgag gcgcaggccg gggtcaggcc
    11041 catgaactcc agccccagcc gcagcccgtg ctcggccgcc agggccccca gccgccccag
    11101 cgaatccacc gcgcgcgcat cctcggcatc gtggatatgg gtaaccgcgc ggcgcgcgcc
    11161 aatggccgcg gcaatctcga agctctcgcg gtaaagcccg gcatcgcccg ccgcggtcac
    11221 cgggaagaac tcgacattgc ccacccccac cccggccgcg tccagccgct cgcgcagccg
    11281 cgccaccagc gggcgggtga ccaccgggaa cagcgttggc gccgcatcgc ccggcagcgc
    11341 cgcgcgcgga atgtgggtga acaggcagac ctcttcgcag ccaacctcgg cggcaaggtc
    11401 aaccagcgtg agcggatcgc attcgttggc ggtaatctgg tgcaggccca gcttcatctg
    11461 cgctctcctc aaaccgcgag cgggccgccg gcccaccagc gggcaaaggc cggttgcaac
    11521 gcctgggaga cgatctcgat caccatcccc cccgcggccg gctggaggta ggtgaacagg
    11581 ccgtagccgt cctcgggcgc ggcgccggcg gcgatcacct gccagcccgc gccgcccagc
    11641 cgcgcgactg tcgccggcag gtcgtccacc cagatgccca ggtgatcccc gcgcgacagg
    11701 gccggatcgt agaacgaacc cggcgtgccc ccctgcagct ccagatgcag cggcccgtcg
    11761 agcgaatagg tgaagcggtt gcgcacggtg tgccggccgc gctccggcgt ccacagcgcc
    11821 agtgcttcca gcaccaccgg ctcggcaaaa cgcagcccca gcgtgcgcgc gtagaaggcg
    11881 atggcagcgt caaggtcggg ggtcagcacc ccggtgtgga acgcgcgggt atagtcgagc
    11941 atcgggctct cctctcgtcg ggtgttcagc aggggccaag gatcacgcgc tcccgcgcga
    12001 gcagcacgcg ctcggccccg gtcagcgccg catcgcgggc ctgccaggcg cgggcggcgg
    12061 tctcggcatc gggccaccac gagctttcga tgcccagcca cgccgccgca tcgctgccct
    12121 cggcctcgcc cgagaggatg cgcgcgcgca ccagccggca gggctcagcc cccgccatgc
    12181 cgatcggctg gtgcgcgccc gcccaggccg cctgcagcgc cggccgcccc ggatcggccg
    12241 ggatcagccg catcagcttg accccatcgg ccgccccatc gcggatcacc gtgtcggcaa
    12301 cgaagaagct gatcgccggc agggtgatga agcgcgggct gtcgacatca taggcatggc
    12361 ggaaaccctc ggccgcgaac aattcggcga gcgcggccgc atcgcggatc cacagctcgg
    12421 gcgcgccatc gaagctgttg gccaggcccg ccggcgccgc cgccagcccg acatcctgca
    12481 catagcccag aatcggcccc ggatcggcaa aggccatcac cagatcgcgg tggaccgtgc
    12541 cccagtaccg ggcgaaatcg gcatggctca tcccggcgcg gcggcggatg aagccgatgt
    12601 agtgg
//
LOCUS       DASRYE010000313         7082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_263828,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000313 DASRYE010000000
VERSION     DASRYE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263828"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VFF98_16135"
     CDS             complement(<1..120)
                     /locus_tag="VFF98_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZU65218.1"
                     /translation="MACISNGLIVGGGVAGLSAAIALQRIGVACDVVEIGEAPA"
     gene            691..2982
                     /locus_tag="VFF98_16140"
     CDS             691..2982
                     /locus_tag="VFF98_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65219.1"
                     /translation="MKTIGYVACRALAGVSALALVAPAHAQEGPAAGVPADAAEIIVT
                     AQKRAENIQKVPISVTLISSDQLERRGVQGIQDLSQASASLEFTAPTASPGGAGFVRG
                     IGTSGIDGATTTSSVSLVLDGVVLGKANITDLFDTDRIEVLKGPQGTLFGSSVSAGVI
                     NITTKAPKLGEVSGYVGGELALDGMGSAYTRKIIRGAINLPVTATSALRLAVQAYDNS
                     GMSHDTYTGGREDLSSIAVRLRYRAELGDKVTFNLIGDYNQTRDNGFSLQTFRAATPG
                     SPLAGALAACGLNVGPDNTSNCQGLPAFYHTAVGGVSGQFDVKLGSATLTSITSWRQR
                     EERTAVDVVEIAPAIARQYLSGCVLPANAQYACSPLTSLVAGSGDDPHSVRNTLITQE
                     LRLASAANHHLEWVAGLYFQRAVYAEALPLTVSINPALPAGVPDIVTVNSTAINAAHA
                     SDYAVFGNVTYFLRPDTRLIGGLRFTHSDVWETTSNAVNLGSSAFFTGTGKADVVTWR
                     AGVQHDFTPATMAYFTAATGYKAPEIDDSKTTMPAIKPEEPLSLEMGIKQTVLHGKFY
                     INADLFYTDVRNFQTQSCVYGLGGLTCSSVNVPHVHTHGLEWDIFGHPFTGTTVNISG
                     IYNIAKYSHGFLGADRSDLGGFQLNYAPRFKMTVSLEQAIPLNRRYTLVLGGDATIRS
                     SESMYLSSSPVFVVPGATLFNARASLKSATNWSLAVFGRNLANKVYPVQLYPTTAYAA
                     GGVWQALDNNAQRQVGLQAEIRF"
     gene            3091..3915
                     /locus_tag="VFF98_16145"
     CDS             3091..3915
                     /locus_tag="VFF98_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU65220.1"
                     /translation="MRKGFVALLSAAWAIMLASAAAMAQADPAASVGGERVVALPLQD
                     GTGLRVLYAAPEHPWATIVMLPGGSGDIGAQEGDGAEGDDIRHGDNFVVRTRALWSAR
                     GYAVLIPDTIAHASLRGWRSSPAYAAVVAQLAAFARTRAAGPVFLLGTSQGSIAAMNG
                     AAHAAPGAIAGVVLTESVSRMGGSHETVFSAHPQAVTAPALIVANRDDACPVAPPEDA
                     PRIAAQLTHAREVRILAVSGGISRSRRACGSLTPHGLYGIEPEVIDGISDWMHRHG"
     gene            4087..4563
                     /locus_tag="VFF98_16150"
     CDS             4087..4563
                     /locus_tag="VFF98_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65221.1"
                     /translation="MYAFVDRPVTELDAGGRLLVWAMRLWVKAVGERKCPGNALAPAF
                     AGLAVLPGLQPFMAMMALFNRHGLENFQFCALPCNHVSEHEALILTLVCTMPGATPKT
                     ARDTLELLVAEEAVAPLVDAVARLGKALGAVGMHPQLPDAAAPQQASGSVSTPTAD"
     gene            4612..5424
                     /locus_tag="VFF98_16155"
     CDS             4612..5424
                     /locus_tag="VFF98_16155"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HZU65222.1"
                     /translation="MSNRKAQLAGLPPSAVLTAQTVLAVRHWNERLFSFSLSRPPSFR
                     FRSGEFVMLGLPGEGRPLLRAYSIASPAYADALEFLSIKVPDGPLTSQLQRMAVGDAL
                     YLGRKPTGTLVADALLPGSRLFLFATGTGLAPFLSIIRDPDIYDRFGQIILVHSVRQV
                     SDLAYRQELESHLAADPLVSDAALLQFTYLPTVTQEAFRTEGRIGTLVEDGRLFAGVA
                     GPRRLDPATDRLMLCGSMAMIRDFTQQLQALGFAEGANARPGHFVIERAFVA"
     gene            5758..5931
                     /locus_tag="VFF98_16160"
     CDS             5758..5931
                     /locus_tag="VFF98_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZU65223.1"
                     /translation="MIVCICNAIREREIRDMARTGLTDVEAIYAELGHEPNCCQCLPF
                     ARQIVEGEVCAPA"
     gene            6006..6485
                     /gene="bfr"
                     /locus_tag="VFF98_16165"
     CDS             6006..6485
                     /gene="bfr"
                     /locus_tag="VFF98_16165"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZU65224.1"
                     /translation="MKGDRKVIEFLNGALKNELTAINQYFLHYRMLAHWGVTTLAKYE
                     YHESIDEMKHADSLASRILFLDGLPNFQDLGRLRIGESVEEILKADLALEMDAVLLLR
                     EAIVHCESVRDFVSRDLFQHILESEEEHIDHLETQFELIERMGIQNYVQMQSAPEKS"
     gene            complement(6549..7004)
                     /locus_tag="VFF98_16170"
     CDS             complement(6549..7004)
                     /locus_tag="VFF98_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZU65225.1"
                     /translation="MSVAFRRETDDEHLEPKFELPIPPGPNLVTARGLAMIHSRVAEL
                     EALLPQLAEEDAIKAARRQLRYWSTRQSTAQVMDKADGAQVAFGCTVTIRMNGKERVI
                     TIVGHDEADPAAGLLSFLAPLSRALMGAEPGETLDFAKEADAIEVIAIS"
BASE COUNT         1184 a         2332 c         2261 g         1305 t
ORIGIN      
        1 ggccggcgcc tcgccgattt ccaccacatc gcaggccacg ccaatgcgtt gcaacgcgat
       61 cgccgcagag agccccgcaa cgcccccgcc aacgatcaac ccattggaaa tgcatgccat
      121 cctttactcc ttgctctgtc cgggggcggc cgcctgcggc ctggccgggg catgggaatg
      181 cgcacggccg cccggcgggg gcgattggcc ctttcgttaa gcctaaccat gttggcatgc
      241 gtatgcaaca tttttgcatg agtgggtgat cagcgaggtg gtttagaatt atataaatga
      301 tattaaatga tttaattagg ttctgtcagg ggtggtcaag ggaagtgccc tgcgaatcat
      361 ccccatcatc agcgttgcat ggtgcgtgtt ttcgcccatt ggcacgccat tccttcgcag
      421 aaatttcgcc atgggttagc cgtttgcgcg ctcatgcaga aaccaaatta ttgcatataa
      481 acggttaaat ggaattctcc cagcgccacc cgtcaaaatt gcgccatgtt gcatgcgcat
      541 gcaaattgag atatcacgct gccaccaaca acaaaacggc cacaggcggg cgctgcgatg
      601 ccggtggcaa aggcgcagcg ggaaaggcct gccgatcctg tgtcggcacc attgcggcgc
      661 gggcctgctg aaaaaccaaa ggggaggatt atgaaaacca tcggatatgt cgcgtgccgg
      721 gcgcttgcgg gtgtatcggc gctggccctg gtcgcgcctg cccatgcgca ggagggccca
      781 gccgccggcg tgcccgccga tgccgccgag atcatcgtga cggcccagaa acgggcagaa
      841 aacatccaga aggtgccgat ctcggtcacg ctcatctcgt ccgaccagct ggagcggcgg
      901 ggcgtgcagg gcatccagga tctgtcacag gcctcggcca gcctcgaatt cactgccccc
      961 accgcctcgc ccggtggcgc cggctttgtg cgcggcattg gcaccagcgg cattgatggc
     1021 gccaccacca ccagctcggt cagcctggtg ctcgatggcg tggtgctggg caaggccaac
     1081 atcaccgatc tgttcgacac cgaccgcatc gaggtgctga aagggccgca aggcaccctt
     1141 ttcggcagct ccgtttcggc gggcgtgatc aacatcacca ccaaggcccc caaattgggc
     1201 gaggtctccg gctatgtcgg cggcgagctg gcgctggatg gcatgggttc tgcctatacg
     1261 cgcaagatca tccgcggggc catcaacctg ccggttacgg ccacatcggc cctgcgcctg
     1321 gcggtgcagg cctacgacaa ttcgggcatg tcgcatgata cctacaccgg cggccgcgag
     1381 gacctgtcct cgatcgccgt gcgcctgcgt taccgggccg agctgggcga caaggtcacc
     1441 ttcaacctca tcggcgatta caaccagacc cgcgacaatg gtttcagcct gcaaaccttc
     1501 cgcgccgcca cccccggctc gccgctggca ggcgcgctgg cggcctgcgg gctgaacgtg
     1561 gggccggata acaccagcaa ttgccagggc ctgcccgcct tctaccacac ggccgtgggc
     1621 ggtgtttcag gtcagttcga tgtcaagctg ggcagcgcca ccctgacctc gatcacctcc
     1681 tggcgccagc gcgaggagcg cacggccgtt gatgtggtgg aaattgcccc ggccattgcg
     1741 cggcaatatc tgtcgggctg cgtgttgccg gccaatgccc aatatgcctg ctcgccgctg
     1801 acatcgctgg tggccggctc gggcgatgat ccgcacagcg tgcgcaacac gctgatcacg
     1861 caggagctgc ggctggcctc tgcggccaac catcacctgg aatgggtggc cggcctctat
     1921 ttccagcgcg cggtctacgc cgaggcgctg ccgctgacgg tgtcgatcaa tccggcgctg
     1981 ccggccggcg tgccggatat cgtcacggtc aacagcaccg cgatcaatgc ggcacatgcc
     2041 agcgattatg ccgttttcgg caatgtgacc tacttcctgc gccccgatac gcggctgatc
     2101 ggcggcctgc gcttcaccca ttccgacgtg tgggaaacca cttccaatgc ggtcaatctg
     2161 ggctccagcg ccttcttcac agggaccggc aaggccgatg tggtgacctg gcgcgcaggc
     2221 gtgcagcatg atttcacccc cgccaccatg gcttatttca ccgccgccac cggctacaag
     2281 gccccggaaa tagacgattc caaaaccacg atgccggcga tcaagcccga agagccgctc
     2341 agcctggaaa tgggcatcaa gcagaccgtg ctgcatggca agttctatat caacgccgat
     2401 cttttctaca ccgatgtgcg caatttccag acccaatcct gcgtgtatgg gctgggcggc
     2461 ctcacctgtt cgtcggttaa cgtgccgcat gtgcataccc atgggcttga atgggatatt
     2521 tttggccatc ccttcaccgg caccacggtc aatatctccg gcatctacaa tatcgccaaa
     2581 tattcccacg gtttcctcgg cgccgatcgc agcgatctgg gcggcttcca gctcaattac
     2641 gcgccccggt tcaagatgac cgtctcgctt gagcaagcca tcccgctgaa ccggcgctac
     2701 acgctggtgc tgggcggcga tgccaccatc cgctcatcgg aatcgatgta tctttcctcc
     2761 agcccggttt tcgtggtgcc tggtgccact ctcttcaacg cccgcgccag cctgaaatcg
     2821 gcgacaaact ggtcgctggc ggtgttcggg cgcaatctgg cgaacaaggt ctatcccgtc
     2881 cagctctacc ccaccaccgc ctatgccgcc ggcggcgtgt ggcaggcgct cgacaataac
     2941 gcccagcggc aggtgggcct gcaggccgaa atcaggttct gacaccccgg gccctgttgc
     3001 cggtggcgac agggccagtg acagggctgg tcaacccgcg cgcatcgggc ccatgatagg
     3061 gggcgccgat ccggcagcga aggggtgatc atgcgcaagg gcttcgtcgc gcttctgtct
     3121 gcggcatggg cgatcatgct ggcttcggct gccgccatgg cgcaggctga tcccgccgcc
     3181 tccgtcggcg gcgagcgtgt tgttgcgctg ccccttcagg atggcaccgg tctgcgcgtg
     3241 ctttatgccg cgcccgagca cccctgggcc acgatcgtga tgctgcccgg tggttcgggc
     3301 gatatcggcg cgcaagaggg agacggcgcg gagggcgatg acatccggca tggcgacaat
     3361 ttcgtcgtgc gcacccgcgc cttgtggagc gcgcgcggct atgccgtgct gattcccgat
     3421 acgatcgcgc atgccagcct gcgcggatgg cgcagttcgc ccgcctatgc cgcggtggtg
     3481 gcgcagttgg ccgccttcgc ccgcacgcgg gcggcgggcc cggtgttcct gctgggcacc
     3541 agccaggggt cgatcgccgc gatgaacggc gccgcccatg cagcgcccgg cgcaatcgcc
     3601 ggggtggtgc tcaccgaatc ggtctcgcgc atggggggca gccacgaaac cgtgttcagc
     3661 gcccacccgc aggccgttac cgcgccggcg ctgatcgtcg ccaatcgcga cgatgcctgc
     3721 ccggtggcac cgccagagga cgcgccgcgc atcgccgcgc agctgaccca tgcgcgcgag
     3781 gtgcgcatcc ttgccgtttc cggcgggata agccgatcgc ggcgggcctg cggatcgctg
     3841 acgccgcatg ggctgtacgg gatcgagcct gaggtgatcg acgggatcag cgactggatg
     3901 caccggcacg gctgaggcgg ttgacggccg cgatttaggc tgtctataaa ctgctattga
     3961 gagtcgttct catttatgag agaagagcgc atccccgccc gcccggcgat cctgccgggg
     4021 ccgcgcgggg gggcgcgccg atcgttccgg gggcaccgtt tcgatttcca ttcaaggagc
     4081 tttgccatgt atgcatttgt cgatcgcccg gtcaccgagc tggatgccgg cgggcgcctg
     4141 ctcgtctggg ccatgcgcct gtgggtcaag gccgtgggcg agcgcaaatg cccgggcaac
     4201 gccctggcgc cagcctttgc ggggctggcc gtgctgccgg gcctgcagcc cttcatggcg
     4261 atgatggcgc tgttcaaccg ccacggcctg gaaaacttcc agttctgcgc cctgccgtgc
     4321 aaccatgtct ccgagcatga ggcgctgatc ctcacgctgg tctgcaccat gcccggggcc
     4381 acgccgaaaa ccgcgcgcga cacgctggaa ctgctggtgg cggaagaggc ggtggcgccg
     4441 ctggtggatg ccgtggcccg gctgggcaag gcgcttggcg ctgtgggcat gcatccgcaa
     4501 ctgccggatg cggccgcccc gcaacaggca tccggctccg tctcgacgcc gaccgcggat
     4561 tgaaacgctg attgcagccc gcgccgggcc cctccttgag gacaattcat gatgagcaac
     4621 cgcaaggccc agctggctgg cctgccccct tcggccgtac tgaccgccca gaccgtgctg
     4681 gcggtgcggc actggaacga acgcctgttc agcttctctc tctcccgccc gccttccttc
     4741 cgcttccgtt cgggcgaatt tgtcatgctg ggcctgccgg gggagggccg cccgctgctg
     4801 cgcgcctatt cgattgccag cccggcctat gccgatgcgc tggaattcct gtcgatcaag
     4861 gtgcccgatg gcccgctcac ctcgcagctg caacgcatgg cggtgggcga tgccctctat
     4921 ctcgggcgca agcccaccgg aacgctggtg gccgatgcgc tgctgccggg cagccggctg
     4981 ttcctttttg ccaccggcac cggccttgcc cccttcctgt cgatcatccg cgatcccgac
     5041 atctacgacc gcttcgggca gatcatcctc gtgcacagcg tgcgccaggt gagcgacctt
     5101 gcctacaggc aggagctgga aagccatctg gccgccgatc cgctggtgag cgatgccgcc
     5161 ctgctccagt tcacctatct ccccacggtc acgcaggagg cgtttcgcac cgaggggcgc
     5221 atcggcacgc tggtggagga tgggcggctg tttgccgggg ttgccgggcc gcgccggctc
     5281 gatcctgcca ccgaccggct gatgctgtgc ggcagcatgg cgatgatccg cgattttacg
     5341 cagcaactgc aggcgcttgg ctttgccgag ggggccaatg ccaggcccgg ccatttcgtg
     5401 atcgagcggg cttttgtcgc gtgaagcggg tggctattgc gaggcactcg catttgatgt
     5461 agaagcaggg cttgtggagt cggatctgcc tgcgagaacg ccatgatcgt atcgccctgt
     5521 gtgtctgtat gccggatcga cgaagccagg ggcttttgcg tgggctgcgg ccgcacccgg
     5581 gcggaaatcg ccgcctggct ggggcagagt gatgactggc ggcaatccgc catccggcag
     5641 gccagcgcgc ggctggcgcc ggcgccgcag cgccgctggc gcctggcaaa gtggctgcgg
     5701 ccgcgttcct gccggatgcc gcggcgttga cctgcagatt ttgaaccgga gtgaccgatg
     5761 atcgtttgca tctgcaatgc catccgtgaa cgcgagatcc gcgatatggc gcgcaccggg
     5821 ctcaccgatg tcgaggccat ctatgccgaa ctcgggcacg agcccaactg ctgccagtgc
     5881 ctgcccttcg cccgccagat cgtcgagggc gaggtctgcg cgccggcctg attttcccgt
     5941 atcgacccgg ttgccccccg cggccatcgg cgtatgctgc gcttccccag tggcaggagg
     6001 cgttcatgaa gggcgacagg aaggtcatcg aatttctcaa cggcgcgctc aagaatgaac
     6061 tgaccgcgat caaccagtat ttcctgcatt accgcatgct ggcccattgg ggcgtgacca
     6121 cgctggccaa gtatgaatac catgaatcga tcgacgagat gaagcacgcc gacagtctgg
     6181 cgagccggat cctctttctt gatggcctgc ccaattttca ggatctgggg cggctgagga
     6241 tcggggaaag cgtcgaggaa atcctcaagg ccgatctggc gctggaaatg gatgccgtgc
     6301 tgctgctgcg cgaggcaatc gttcactgcg aaagcgtgcg cgattttgtc tcgcgcgatc
     6361 tgttccagca catcctcgaa agcgaggaag agcatatcga ccatctcgaa acccagttcg
     6421 agctgatcga gcggatgggc atccagaatt acgtccagat gcaatcggcg cccgagaaaa
     6481 gctgagcccg gccgcgccaa gggctgccgg gcacgccgcc ggaaaatggc ggcggcgcct
     6541 gggcatcgtc agctaatggc aatgacctcg atcgcgtctg cctccttcgc gaaatcgagc
     6601 gtctcgccgg gctcggcgcc catcagggcc cggctcagcg gggccaaaaa ggagagcagg
     6661 cccgccgcag gatcagcctc gtcgtggccg acgatggtga taacgcgctc cttgccgttc
     6721 atccggatgg tgacggtgca gccaaaggca acctgcgcgc catcggcctt gtccatcacc
     6781 tgcgcggtgg attgccgcgt gctccaatag cgcaactggc gccgcgcggc cttgatcgcg
     6841 tcttcctcgg ccagttgcgg cagcagggct tccagttcgg ccacccggct gtggatcatc
     6901 gccagcccgc gcgccgtcac caggttcggg ccgggcggga tcggcagttc gaactttggc
     6961 tccaagtgtt cgtcgtcagt ttcgcggcga aaggctacgc tcatgtgcag gtctactcca
     7021 accggggccg ggcgcgcggt ttcgggtgtc tgcgcgaaca aagcaagccc cccccccccc
     7081 cc
//
LOCUS       DASRYE010000314         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_35339,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000314 DASRYE010000000
VERSION     DASRYE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35339"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFF98_16175"
     CDS             complement(<1..895)
                     /locus_tag="VFF98_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU65226.1"
                     /translation="MLVAYAALAGPTVHAASLRPDQQAFMATYRELVETNTELSDGDC
                     TLAAQRMGARLAAAGLPAADITVFSVPDHPREGGLVAVLPAASGQHRRPILLLAHIDV
                     VEARAADWQSDPFRLTEKDGYFYGRGASDDKAMAASWVDTLVRLRQQHRRLARPVKLA
                     LTCGEETEGAFNGAQWLSTHQRGLIDAAFALNEGGAGQLDAAGHRLFLGVEAEEKARQ
                     DFRLEAKGEGGHSAMPAADNAIARLAAAVGRVAALDFPVRLDSVSRAYLAARARLAGG
                     ADAAAISAFLAHPEDPAAQAPL"
     gene            complement(934..1551)
                     /locus_tag="VFF98_16180"
     CDS             complement(934..1551)
                     /locus_tag="VFF98_16180"
                     /inference="COORDINATES: protein motif:HMM:NF005754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HZU65227.1"
                     /translation="MSARRIFQIGTSGTIGKAVAEAIRARGHELVSADRDEGPLRIDI
                     TDTASIAAAWAAVGAVDAVVCTVGVVPLKPLAEWRRADIDEAVAGKLASQIDIVLQGM
                     AHVRTGGSFTLISGIMSRMPWHGGIGATIVDGGIDAFVMGAAAEIGKDRRINAISPTI
                     VQESVDLIGRNLLPGHRPVPAAEVAGAYLRAIEGIETGKVFILGE"
     gene            complement(1548..2063)
                     /locus_tag="VFF98_16185"
     CDS             complement(1548..2063)
                     /locus_tag="VFF98_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65228.1"
                     /translation="MSLTEQELLDRAEITDALVAYCQAQDQNAWPLYARAFTADATIA
                     FPGMGMEPMAASVFRDFLIRFNETRLSGQHLIGNSWFEISGDEACVVSEAIYITLHPT
                     DTPGRIRRVRGNALYTDRCRRTAEGWRIAERVIAQKNVEVDEGDYDPALLEAIRAAAA
                     TDWFTSAGAGQ"
     gene            complement(2060..2587)
                     /locus_tag="VFF98_16190"
     CDS             complement(2060..2587)
                     /locus_tag="VFF98_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65229.1"
                     /translation="MALTIAALLARAAIHDCLNRYAQGVDQRNWPLFLSAFAPGAVVV
                     VQGYLAAPITPEGFAALLRDTFDHNRLSGQHLLGNTALAVEGARAHAVTEFLATTLEE
                     GAAPATAARQITAGLYVDDLVCGDDGHWRIARRTLARKSSNEDQLPFLPPVADAVRAT
                     LRAGWPLASPTGTPA"
     gene            complement(2591..3310)
                     /locus_tag="VFF98_16195"
     CDS             complement(2591..3310)
                     /locus_tag="VFF98_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZU65230.1"
                     /translation="MKISVLGATGGTGQAIVQQALDAGHAVTALVRRPEALAVRGDQL
                     TVLVGDARDPAIIEQLVAGHDAVICSLGLPAAGTTRAEINDEVRADVCTVSTRLLFAA
                     MPRHGLRRIVLMSTHGAGGSNDGSPYVRWLRDLVQNRVLDKDDMEAFIAASDAPVDWT
                     VIRNPAIYEGPKGLPHAVYTRVELNRSSKITYADLAAFAVAEVEQPRHVGQFLTITEP
                     MDDAALMAAAEVAPVVAPVRA"
     gene            complement(3307..>4427)
                     /locus_tag="VFF98_16200"
     CDS             complement(3307..>4427)
                     /locus_tag="VFF98_16200"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZU65231.1"
                     /translation="LDYDDGHYEGGGIHLGAVIVPALLAALPDAPPAQDPPVNELLAA
                     LAAGYEVGARAGWLLAPRAPGDPYHTSGAAGAMGAAAAVARLLGLDAAGCLAALRIAS
                     AHAPIAGLQLPMVKEAIGWGAATGLAAAQLAAAGFGGGFGAVSGAQDPAEAPLGIAPT
                     PFDGPHGRAPFALGWGQAWHSLNAYIKPWPCCRAIHTAIAGVEELLARAGAPATAIER
                     IEVEVPAGCAGLSFLPPASMDHAQFSLPFVLGCLMVHGAVRPAHFLGAGLADPAVLAA
                     AARVHLRADPALAPLSSATSYPARVALTIGGAVWRLAVDHAPGSRARPLGRAAVAAKL
                     AEAAALAGLPADAIPAPGRLPRRADLFALFPPPTEMNL"
BASE COUNT          687 a         1604 c         1528 g          608 t
ORIGIN      
        1 gcaagggggc ttgcgccgcc gggtcttccg gatgggcgag gaaagcgctg atcgccgctg
       61 catcggcgcc gcccgccagc cgggcgcgag cggccagata ggcccggctg acactatcca
      121 gccgcaccgg aaaatcgagc gcggccaccc ggcccaccgc cgctgccagc cgggcgatgg
      181 cattatccgc cgccggcatg gcgctgtggc cgccctcgcc tttcgcttcc aggcggaaat
      241 cctgccgcgc cttctcctcg gcctccacgc ccaggaacag gcggtggcca gccgcatcca
      301 gctggcccgc cccgccctcg ttcagcgcga aggcggcatc gatcagcccg cgctggtggg
      361 tgctcagcca ctgggcgccg ttgaaagcgc cttcggtttc ctcgccgcag gtcagcgcca
      421 gcttcaccgg gcgcgccagc cggcgatgct gctggcgcag gcgcaccagc gtatccaccc
      481 agctggcggc catggccttg tcgtcgctgg cgccgcggcc gtagaaatag ccgtccttct
      541 cggtgaggcg gaagggatcg ctctgccagt cggccgcgcg cgcttccacc acatcgatat
      601 gggccagcag caggatcggc cgccgatgct ggccagaggc cgccggcagc actgccacca
      661 gcccgccttc ccgcgggtga tcgggcacgg aaaagacggt gatatccgcc gccggcaggc
      721 ccgctgccgc cagccttgcc cccatgcgct gcgccgccag cgtgcaatcg ccatcggaaa
      781 gctcggtatt ggtttccacc agttcccgat aggtggccat gaaggcctgc tgatcggggc
      841 gcagtgatgc cgcgtgtacg gtcggcccgg ccagcgccgc gtatgccacc agcatcatgc
      901 cccccatcag ccccagcgcc cgcctgccca tagctattct cccaggatga acaccttgcc
      961 cgtctcgatc ccctcgatcg cgcgcagata ggcgccggcc acttcggcgg ccggcacggg
     1021 cctgtgcccg ggtagcagat tgcggccgat gagatcgacc gattcctgca cgatggtggg
     1081 gctgatggcg ttgatccgcc ggtcttttcc gatttcggcc gccgccccca tgacaaaggc
     1141 gtcgatcccg ccatcgacaa tggtggcgcc aatgccgcca tgccagggca tgcgcgacat
     1201 gatccccgag atcagcgtga aggagccgcc cgtccgcaca tgcgccatgc cctgcagcac
     1261 gatgtcgatc tgcgaggcga gcttgccggc cacggcctcg tcgatatcgg cgcggcgcca
     1321 ttcggccagg ggtttgagcg gcaccacccc caccgtgcag accaccgcat ccaccgcgcc
     1381 caccgccgcc caggccgcgg cgatggaggc cgtgtcggtg atgtcgatgc gcaaggggcc
     1441 ctcgtcgcgg tcggcgctca ccagctcgtg gccgcgcgcg cggatggctt cggccaccgc
     1501 cttgccgatc gtgcccgacg tgccgatctg gaagatccgc cgggcgctca ctggccggct
     1561 ccggcgctgg tgaaccagtc ggtggcggcg gcagcgcgga tggcctcgag cagggccggg
     1621 tcgtaatcgc cctcgtccac ttccacattc ttctgggcga tcacccgctc ggcgatgcgc
     1681 cagccctcgg cggtgcgccg gcagcggtcg gtgtagagcg cattgccgcg cacgcggcgg
     1741 atgcggccgg gcgtgtcggt ggggtgaagg gtgatgtaga tcgcctcgga gacgacgcac
     1801 gcctcatcgc cgctgatctc gaaccagctg ttgccgataa gatgctggcc agacaggcgc
     1861 gtttcattga agcggatgag gaaatcgcgg aaaacggaag cggccatcgg ctccatcccc
     1921 atgccgggga aggcgatggt ggcatcggcg gtgaaggcgc gcgcgtaaag cggccaggcg
     1981 ttctgatcct gcgcctggca ataggcgacc agcgcgtcgg tgatctcggc gcggtcgagc
     2041 agctcctgtt cggtaaggct catgcggggg ttcctgtggg agaagcgaga ggccagccgg
     2101 cgcgcaaggt ggcgcgcacg gcatcggcaa cggggggaag gaagggcagc tggtcctcgt
     2161 tgctgctttt gcgcgcaagg gtgcgccgcg cgatccgcca gtggccatca tcgccgcaga
     2221 ccagatcatc aacataaagc ccggcggtga tctggcgggc ggcggtggcg ggcgcggccc
     2281 cctcctccag cgtggtggcg agaaattcgg tgacggcatg ggcgcgcgcg ccctccaccg
     2341 ccagcgccgt gttgcccagc aggtgctggc cggaaaggcg gttgtggtcg aatgtgtcgc
     2401 gcagcagggc ggcaaagccc tccggcgtga tcggcgcggc cagatagccc tgcaccacca
     2461 ccaccgcccc tggcgcaaag gcgctgagga acagcggcca gttgcgctga tccacccctt
     2521 gcgcatagcg gttgaggcaa tcgtgaatgg cagcgcgcgc cagcaaggcg gcgatggtca
     2581 gcgccacggc tcaggcccta accggcgcca ccaccggcgc cacttcggcc gccgccatca
     2641 gcgcggcatc gtccatcggc tcggtaatcg tcaggaactg gccgacatgg cgcggctgtt
     2701 ccacttcggc cacggcaaag gcggccagat cggcataggt gattttcgag gaacggttca
     2761 gctccacccg cgtataaacg gcatggggca ggcctttggg gccttcatag atcgccggat
     2821 tgcggatcac cgtccagtcc accggcgcat cgctggcggc gatgaaggct tccatgtcgt
     2881 ccttgtcgag cacgcggttc tgcaccagat cgcgcagcca gcgcacatag ggcgagccat
     2941 cattgctgcc gcccgcgcca tgggtgctca tcagcacgat gcggcgcagg ccatggcgcg
     3001 gcatggcggc aaacagcagc cgggtggata cggtgcagac atcggcgcgc acctcgtcgt
     3061 tgatctctgc gcgggtggtg ccggccgccg gcaggcccag gctgcagatc accgcatcat
     3121 ggccggccac cagctgctcg atgatcgccg gatcgcgcgc atcgcccacc agcacggtca
     3181 gctgatcgcc ccgcacggcc agcgcctctg gccggcggac cagcgcggta acggcatggc
     3241 cggcatccag cgcttgctgc acgatcgcct gaccggtgcc gccagtggca cccaatacgc
     3301 tgattttcat aggttcatct ccgtgggagg gggaaacagg gcgaacaggt cggcgcggcg
     3361 cggcaggcgg cccggcgccg ggatggcatc ggccggcaag cccgccagcg ctgccgcctc
     3421 ggccagcttg gcggccaccg cggcccggcc cagcggccgc gcccggctgc ccggcgcatg
     3481 atccaccgcc agccgccaca ccgcgccgcc gatcgtcaac gccacgcggg ccggatagct
     3541 ggtggccgac gacagcggcg ccagcgccgg atcggcgcgc agatgcaccc gggcagcggc
     3601 cgccagcacc gccggatccg ccagccccgc gcccaggaaa tgggccgggc gcacagcgcc
     3661 atgcaccatc aggcagccca gcacgaaagg caggctgaac tgggcatggt ccatcgaggc
     3721 gggcggcagg aaggacaggc ccgcacagcc cgccggcacc tccacctcga tccgctcgat
     3781 agcggtggcg ggcgcgccgg cgcgggccag cagttcctcc accccggcga tcgcggtgtg
     3841 gatcgcccgg cagcagggcc agggcttgat ataggcattc aggctgtgcc acgcctgccc
     3901 ccagccaagg gcaaagggcg cgcggccatg gggcccgtcg aacggggtgg gggcaatgcc
     3961 cagcggcgct tcggccggat cttgcgcgcc agagacggcg ccaaagccgc ccccaaagcc
     4021 agccgcggcc agctgcgcgg cggccagacc ggtggccgcg ccccagccga tcgcctcctt
     4081 caccatcggc agctgcaggc cggcgatcgg cgcatgggcg ctggcgatgc gcagcgctgc
     4141 caggcagcct gccgcatcca gccccaaaag ccgcgccacc gccgctgccg cgcccatggc
     4201 gcccgccgcc ccgctggtgt gatagggatc gcccggcgcg cgcggggcca gcagccagcc
     4261 cgcccgcgcg cccacctcat agccggccgc cagcgccgcc agcagctcgt tcaccggcgg
     4321 gtcctgtgcc ggcggggcat cgggcagggc cgccagcagc gccggcacga tcaccgcgcc
     4381 caggtgaatg ccgccgccct cgtaatggcc atcatcataa tcgagcg
//
LOCUS       DASRYE010000315         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_264241,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000315 DASRYE010000000
VERSION     DASRYE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264241"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFF98_16205"
     CDS             complement(<1..138)
                     /locus_tag="VFF98_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZU65232.1"
                     /translation="MKILVPVKRVIDYNVKPRVKADGTGVDLSNVKMSMNPFDEIAVE
                     EG"
     gene            complement(236..1453)
                     /locus_tag="VFF98_16210"
     CDS             complement(236..1453)
                     /locus_tag="VFF98_16210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65233.1"
                     /translation="MNRQPPLAPAHAPGASPTTLAGLLACGLLPSIGLFAPGLVLPAM
                     ARHFAASPQAPLLTELVGTLASFAFALGAPLAGRLVARRGGRAVILPGLVLFALCGTL
                     PALLDDLWLILAARAALGLALAGIFTGALSGIASLPESQRARMFGGFSMAGSAAAIAL
                     FPIVGLLGHASWRLAFLVHLVALPVLLLAIALPPALGRAAPAAARHGPELPLNAPLLN
                     APLLRLLAVAAIVGMGMLVGPICAPLYLAGLGVSDTRVLAIPATLGSIASVFASASYG
                     WLHPRIGPGGITLLALLVSTAALAIAGSSGDVLVFCAMVVVQSATVALMAPNISATAL
                     ACSPPDRSGEAMGLANGMMFGAQLAFPFLAALVRAHGGIGAVFLILAALAGAAAMMNL
                     YGARAPRPAGAAQ"
     gene            1680..2324
                     /locus_tag="VFF98_16215"
     CDS             1680..2324
                     /locus_tag="VFF98_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZU65234.1"
                     /translation="MKTLVLMRARAAGLEAWLLRDLALRPWLADAPQLAVYLPAPDGP
                     ALYDAVIEVRAGPGAAAAALADADLAARAQVWAGACEELIGKGEARWPAAAGMAAGPV
                     PGVALLSFLRPLPGMERAAFLGHWRAHVALARAVHWGMNRYGQNRMAEPLGEGEEEGW
                     LGMAHLHFPDAHSLRHGLFRTEADKALIAADVAGFVGAHQTMEARWHILRNASA"
     gene            complement(2371..3297)
                     /locus_tag="VFF98_16220"
     CDS             complement(2371..3297)
                     /locus_tag="VFF98_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65235.1"
                     /translation="MKRLSGKVAVVTGAGRNIGRAEALLLAEQGAKVVVNDLGGGPYG
                     TEAGDAALAESVAQEIRAGGGEAVAQFSSVATREGAEAAVQAAIDSFGRIDILINNAG
                     IVRPSRIDRMSDEDWRLCMDVSLTASFHTCRAAARHMIDQRCGVIVNTGSPSGFGHWG
                     MANYSAAKEGLLGFTRSIARDLGEFGIRANLIRPVSHITTTYTPEIQQTLDEATRLGQ
                     SLVWNKPVTMKPLMTALPEHVAALVVWLCSDAAGHISGRDFFIAGDEVALVPEPEAIR
                     TTIHPGGWTLDAFDEPATREYLFGDIRNRFVR"
     gene            3455..4369
                     /locus_tag="VFF98_16225"
     CDS             3455..4369
                     /locus_tag="VFF98_16225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU65236.1"
                     /translation="MRAIVMTAYGDADVLRLAVLPVPAPGPGEVLVRVRAAAVNPADG
                     KWRAGLFAAMAPLSLPHVLGYDIAGEVVAGDGFAVGAQVFGMLDPFRKGGYAEYAVAR
                     AADLALMPPGMAAATAAAIPTAGLTGRQMAAALALRPGQQVLVTGAVGAVGRFALHAT
                     RAAGAQVVAAVRPGQQAAALAAGAASAAPLEGGEWQEKDWQGAPFDHVLDTVGGAAVG
                     RWCRHLRPGGRILSAATTPIAGEHLPAVPEFFTVAPSAGDLAALGRAVAAGEVAVPVA
                     CCLPLAAAATAQRQVEAGGLAGKIVLLP"
     gene            4512..>4767
                     /locus_tag="VFF98_16230"
     CDS             4512..>4767
                     /locus_tag="VFF98_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU65237.1"
                     /translation="MSLIGDFYQIGVVVRDIDEGMERYRRLLGLGPFFRLDTDYEGRY
                     RDWRGRIANRNAFTRWGAAYLEMIEPGVGESNAKEWLHTRG"
BASE COUNT          687 a         1559 c         1826 g          695 t
ORIGIN      
        1 cccttcttca accgcgatct cgtcaaacgg gttcatgctc atcttcacgt tcgacaggtc
       61 cacacccgtc ccgtccgcct tcacccgcgg cttcacgttg tagtctatca cacgcttcac
      121 cggcaccagt atcttcatgg ccatctcctg catcgcggcc cttgcatatt gcggttgcga
      181 agggcggcaa ggcgggcggt gtgttcatca gatagtcgat gagcgcggca ggggtttatt
      241 gggcggcgcc ggccggccgg ggggcgcgtg cgccatacag gttcatcatg gcggcagcgc
      301 ctgccagcgc tgccagaatg aggaacacag cgccaatgcc gccatgcgcg cgcaccagcg
      361 cggcgaggaa gggaaaggcc agctgcgcgc cgaacatcat gccattggcc agccccattg
      421 cctcgccgct gcgatcgggc gggctgcagg cgagcgccgt ggcgctgatg ttgggtgcca
      481 tcagcgccac ggtggcgctt tgcaccacca ccatggcgca aaacaccagc acatcgccgc
      541 tggatccggc gatggcgagg gccgcggtgc tgaccagcag cgccagcagg gtgatgccgc
      601 cagggccgat gcgcggatgc agccagccat aggaggccga ggcaaacacc gaggcgatcg
      661 agcccagcgt ggccgggatc gccagcacgc gcgtgtcgct cacgcccagc ccggcaagat
      721 agagcggcgc gcagatcggc cccaccagca tgcccatgcc aacgattgcc gccaccgcca
      781 gcaggcgcag caggggggcg ttcagcagcg gggcgttcaa tggcagttcc gggccatgcc
      841 gggcggcggc aggcgcggcg cggcccagcg ccgggggcag ggcgatggcc agcaggagca
      901 ccggcagcgc caccagatga acgaggaaag ccagccgcca gctggcatgg cccagcaggc
      961 cgacgatggg aaacagcgcg atcgccgccg cgctgccggc catggaaaag ccgccgaaca
     1021 tgcgcgctcg ctggctttcc ggcaggctgg cgatgcccga tagcgcaccg gtgaaaatgc
     1081 ccgccagcgc cagccccagc gccgccctgg cggcgaggat cagccacagg tcgtcaagca
     1141 gggcgggcag cgtgccgcac agggcaaaca gcaccagccc cggcaggatg accgcccgcc
     1201 cgccccgccg cgccaccagc cggccggcca gcggcgcgcc cagcgcgaag gcaaagctgg
     1261 ccagcgtgcc gaccagttcg gtcagcaggg gggcctgggg gctggcggcg aagtggcggg
     1321 ccatggcggg cagcaccagc ccgggcgcga acaggccgat gctgggcagc agcccgcagg
     1381 ccagcaggcc ggcaagggtg gttgggctgg cgccgggggc atgggccggc gcaagggggg
     1441 gctggcggtt catcgtcctg tctcccgcag ccgggcttgt gggcttggcg cgcgcccgtg
     1501 ctgcctttct cgcccgcttg gtttcagagc gggggcggca tggcaagcgg gcgtagcgct
     1561 ccggcccgcg attgagcgca tgtgtgatgc atggcgggca gggcctgggt cacaggttcc
     1621 ggccgggggc ggcatgcgtc taggctggcg ccggtggagg gccacagggg gagaagggca
     1681 tgaagacgct ggtgctgatg cgggcgcgtg ctgcggggct ggaggcatgg ctgctgcgcg
     1741 atctggcctt gcggccgtgg ctggcagatg cgccgcagct ggcggtctat ctgccagcac
     1801 ccgatgggcc ggcgctgtat gatgcggtga tcgaggtgcg ggcggggccg ggcgccgcgg
     1861 cagcggcgct ggccgatgcc gatctggccg cccgcgccca ggtgtgggcg ggcgcttgcg
     1921 aggagttgat cgggaaaggg gaggcccgct ggccggcggc cgcaggaatg gcagcagggc
     1981 cggtgccggg cgtggccttg ctgtcgttcc tgcgcccgct gccagggatg gagcgcgccg
     2041 ctttcctggg ccattggcgc gcgcatgtgg cgctggcccg tgctgttcac tggggcatga
     2101 accgctatgg ccagaaccgc atggcagagc cgctggggga aggggaggag gaaggctggc
     2161 tgggcatggc ccatctgcat ttccccgatg cgcacagcct gcgccacggc ctgttccgca
     2221 ccgaggcgga caaggcgctg atcgctgccg atgtggccgg cttcgtcggc gcgcatcaga
     2281 cgatggaggc gcgctggcac atcctgcgca atgccagcgc ataggcggcg cccgcagggg
     2341 gcggggggcg atcaggcccc ccgccggcga tcagcgcacg aagcggttgc ggatgtcgcc
     2401 gaagagatat tcgcgcgtgg ccggctcgtc gaaggcgtcc agcgtccagc cgccggggtg
     2461 gatggtggtg cggatggcct cgggctcggg caccagggcc acctcgtcgc cggcgatgaa
     2521 gaaatcgcgc ccgctgatat gcccggccgc atcgctgcac agccacacca ccagcgctgc
     2581 cacatgttcg ggcagggcgg tcatcagcgg cttcatcgtc accggcttgt tccacaccag
     2641 cgactggccg aggcgggtgg cctcatccag cgtctgctgg atctcgggcg tgtaggtggt
     2701 ggtgatgtgc gagaccgggc ggatgaggtt ggcgcggatg ccaaattcgc ccagatcgcg
     2761 cgcgatcgag cgggtgaagc ccagcagccc ttccttggcg gccgaataat tggccatgcc
     2821 ccaatggcca aagcccgaag gcgagccggt gttgacgatc accccgcagc gctggtcgat
     2881 catgtggcgg gcggcggcgc ggcaggtgtg gaagctggcc gtcaggctca catccatgca
     2941 caggcgccag tcctcgtcgc tcatccggtc gatgcgcgag gggcgcacga tgccggcgtt
     3001 gttgatgagg atgtcgatcc gcccgaagct gtcgatcgcg gcctgaacgg cagcttcggc
     3061 cccctcgcgc gtggcaacgg aggagaattg cgccaccgcc tcgccgccgc ccgcgcggat
     3121 ttcctgcgcc acgctttcgg ccagcgcggc atcgcccgct tctgtgccat aggggccgcc
     3181 gcccagatcg ttgacgacca cttttgcgcc ctgttcggcc agcagcagcg cttcggcccg
     3241 cccgatattg cgccccgccc cggtaaccac ggcaaccttg ccagacagtc ttttcatctt
     3301 gttcctcgcc catcgtgaag cgtttactgc atagggcggc ggcgcgcccg tgcctatggg
     3361 caagcgggcg ggctttgcgc catcatggct gtgatggctg ggcggggcgg cttggccggg
     3421 cccgccgccc cgctagattg ccggccctat ggacatgcgt gcgatcgtga tgacggccta
     3481 tggcgatgcc gatgtgctgc gcctggcggt gctgccggtg cccgcgccgg gcccgggcga
     3541 ggttttggtg cgggtgcgcg cggcggcggt gaacccggcc gatggcaagt ggcgggcggg
     3601 cctgtttgcc gccatggcgc cgctcagcct gccgcatgtg ctgggctatg acatcgccgg
     3661 cgaggtggtg gcgggcgatg gctttgccgt gggcgcgcag gtgttcggca tgctcgatcc
     3721 gttccgcaag ggcggctatg ccgaatatgc cgtggcgcgc gcggccgatc tggcgctgat
     3781 gccgccgggc atggcagccg ccacggccgc ggccattccc accgccgggc tcaccgggcg
     3841 gcagatggcg gcggcgctgg ccctgcggcc gggccagcag gtgctggtga caggcgctgt
     3901 gggggcggtt ggccgctttg ccctgcatgc gacgcgggcg gccggcgcgc aggtggtggc
     3961 cgccgtgcgc cccgggcagc aggcagcggc gctggccgcg ggggctgcca gcgctgcccc
     4021 gctggagggc ggggagtggc aggaaaagga ttggcagggc gccccgttcg atcatgtgct
     4081 cgacacggtg ggcggtgcgg cggtggggcg ctggtgccgc catctgcggc cgggcgggcg
     4141 gatcctttcg gcggccacca cgccgatcgc cggagagcac ctgccggcgg tgccggaatt
     4201 cttcaccgtg gcgccatcgg ccggggatct ggcggcgctg ggccgggcgg tggcggcggg
     4261 cgaggtggcc gtgccggtgg cctgctgcct gccgctggcg gccgcggcca cggcgcagcg
     4321 gcaggtggaa gcgggcgggc tggccggcaa gatcgtgctg ctgccctgat gggtgccatc
     4381 agatgagtga tgactggccg gccgtgggaa aagcgccgct cgatgcgcga aaccatccgc
     4441 cccgcagcgc ccgccacaag gcatgctgtc caccacgaca cagcaccagc ggccagcagg
     4501 gagagagcga catgtcattg atcggggatt tctatcagat cggcgtcgtc gtgcgcgata
     4561 tcgacgaggg catggagcgc tatcgccggc tgctggggct ggggccgttc ttccggctgg
     4621 ataccgatta cgagggccgc taccgcgact ggcgggggcg gatcgccaat cgcaatgctt
     4681 tcacccgctg gggcgcggcc tatctggaaa tgatcgagcc gggggtgggc gaaagcaacg
     4741 cgaaggaatg gctgcacacc cgcggcg
//
LOCUS       DASRYE010000316        15727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_188651,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000316 DASRYE010000000
VERSION     DASRYE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15727
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188651"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFF98_16235"
     CDS             complement(<1..876)
                     /locus_tag="VFF98_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HZU65238.1"
                     /translation="MANDLFSHLHFDPAQGHIWLAGRRMMLLHARAFGLLRYEVMQAL
                     GLEKARGLFTRQGYNSGAMDAEIAARLRPTASIDDMFAVGPQLHTLEGMVRAEPVDVR
                     FDTAKGEFAAEYLWKSSSECTAHVEIFGRGAHPAGWSQTGYASGYASVFLGRPILFRE
                     VECIAMGHACCRVVGRPAEEWEEGEADLRYMRGEQLNQMERHPPVFHGKAERRIVGAS
                     AGFNVAFDLLRHVADTDAVVLFLGESGVGKEIFARNLHAMGRRAEKPFVAVNCAAIPE
                     SLLEAELFGVEKGAFT"
     gene            1259..1582
                     /locus_tag="VFF98_16240"
     CDS             1259..1582
                     /locus_tag="VFF98_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65239.1"
                     /translation="MELPFQPLTRRTFLSASALASAAIAAPAAAMALNARPLALASSH
                     QALPASWQGPALVLSGDYLARLGQLRQALESHAPASCRLFLDDADRVLFDVAAHAPHA
                     KGFAA"
     gene            1579..1968
                     /locus_tag="VFF98_16245"
     CDS             1579..1968
                     /locus_tag="VFF98_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZU65240.1"
                     /translation="MIRRPVSRAASRPAARVASLAAGALGLGGVLAAVLAGPAATPAQ
                     ASPTGVWTSPQEAYDKICARCHLSGVGPELRGRALPPEYIKLIVRSGRLAMPAFPHSS
                     IDDATLDGVARLVSAQKLPKPAAAPKR"
     gene            1983..3536
                     /locus_tag="VFF98_16250"
     CDS             1983..3536
                     /locus_tag="VFF98_16250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU65241.1"
                     /translation="MTDQTTPAVLPHGVSTADFRAAIAEFIAILGAANVMTDEAHIAP
                     YTKVMVAESEDLHRPSAILYAREVGEIQKILAVCNTYKVPIWTISTGRNFGYGSAAPQ
                     SAGQVVLDLRHMNKIIEVDPVLCTALVEPGVTYQQLKDYLVEHNIPLWLSCPAPSAIA
                     GPLGNTVDRGVGYTPYGEHFMMQCGMEVVLANGEVLRTGMGGVEGTSAWQVFKWGYGP
                     YLDGIFTQSNYGIVTKMGMWLMPQPPVYKPFCIRYEHDEDIHDIVETLRPLRIANVIP
                     NAMVFANVMWEAAAMMPRSKYYSGTGTTPDHVLDEIKAKEGLGAWNVYAALYGTKEQV
                     EVNWAIITGAIKASGKGRIITEEEAGETEPFKYRAKLMRGEMTLQEFGLYRWRGGGGS
                     MWFAPVTAAKGSETVEQTRLAKQILGEYGLDYVAEYIVGMRDMHHIVDVLFDRSDPEE
                     MKRAHDCFAKLLSEFGKRGYAVYRVNTAFMDQTADLYGPVKRKVDQTLKRALDPNGIL
                     APGKSGIRI"
     gene            3568..3954
                     /locus_tag="VFF98_16255"
     CDS             3568..3954
                     /locus_tag="VFF98_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZU65242.1"
                     /translation="MPHLIGRAFGAALLPLALAATPAAAQSGDATRGRAVFQGQCGVC
                     HSVAPGKAIIGPSLAGIVGRAAGKQAGFAYSPALAKAGFKWDKAKLDAWIAGPAKVVP
                     GNRMPFAGLADGAKRADLIAYLANPK"
     gene            3951..5414
                     /locus_tag="VFF98_16260"
     CDS             3951..5414
                     /locus_tag="VFF98_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU65243.1"
                     /translation="MSGGGLPVALLIDGADRPASHGATFTRHGPLGDGPASTAAAASV
                     EDAAAAAEAAQAALPAWAALGPNARRRLLLAAADAMEARAGDFVAAMAAETGASAGWA
                     GFNVHLGASLLREAAALTTQVKGETIPSDRPGCHAMTLRRPAGVVLGIAPWNAPVILG
                     VRAIATPLACGNTVVLKASEHCPATHRLIGTVLQEAGFPAGVVNVLSNAPEDAGAVVA
                     ALVRHKAVRRINFTGSTRVGRIIAGLAAQELKPCLLELGGKAPLIVLDDADVEAAVAA
                     AGFGAFMNSGQICMSTERIILQGGIADAFVALFRQKVASLHAADPRQGAAPLGSVVDE
                     ATADRIRHLIADARAKGAVVYGEVPATGTLLPAIALDHVTPEMAIYAEESFGPVTTIL
                     RAADDEEALRLANDTEYGLTSAVFGRDLARALSVAERIEAGMCHINGATVHDEAQMPF
                     GGTKASGYGRFGGEAGIAEFTELKWLTIDTGTPHWPI"
     gene            5489..6454
                     /locus_tag="VFF98_16265"
     CDS             5489..6454
                     /locus_tag="VFF98_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZU65244.1"
                     /translation="MIKTRKLALAMALSAAAIAHPAAASESGGGVYPVGAEGALGGAL
                     PPPGVYLLGYLQSYEAGRFNDGKGNAGFLPDFHVSAEAAVTRVVWNTGRKFLGADYAM
                     HLVAPAVVIDARVAGVSDRRAGFTDVTIDPFILGWHWKSGFHIITGLDINLPVGSYSR
                     TSPANLSRHYWNVEPIIAAAYYGPHGLQVDMKAMYDINFTNHDAQINGLNPTGSPYRS
                     GNEFHMEYAVTKALAPRLSAGIAGYYYRQTTRDHVDNTDAQQLLDSFGGLKGEAVAAG
                     PTLRLTAGKAQIIATWQHEFDAAYRPQGDKVWLKAIIPLAGVGKR"
     gene            complement(6550..6635)
                     /locus_tag="VFF98_16270"
     tRNA            complement(6550..6635)
                     /locus_tag="VFF98_16270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6600..6602),aa:Leu,seq:caa)
     gene            complement(6755..7573)
                     /locus_tag="VFF98_16275"
     CDS             complement(6755..7573)
                     /locus_tag="VFF98_16275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZU65245.1"
                     /translation="MAISGMIRVMERAARKAGQRLRRDFGEVEHLQVSRKGPADFVSR
                     ADQASERTLYEELRAARPDWGFVMEEGGTVEGDPTKPRWIIDPLDGTSNFLHGIPHFA
                     ISIAAQEPLPGGEGWGEVVAGLVYQPITDESFWAEKSRGAWLHDRRLRVSGRRHLDES
                     LIATGIPFAGHGDVVEWSRIYQALAPQVAGIRRFGAAALDLAWVAAGRYEGFWESSLK
                     PWDTAAGCLLVREAGGFVSNWKGQSLPICDAEVLAGNDALHSRLHKILAGALRD"
     gene            complement(7580..8143)
                     /gene="efp"
                     /locus_tag="VFF98_16280"
     CDS             complement(7580..8143)
                     /gene="efp"
                     /locus_tag="VFF98_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZU65246.1"
                     /translation="MKISGVEIRPGNILEYEGGIWKVAKTQHTQPGKGGAFMQVEMKN
                     LIDGRKTNVRFRSADTVERVRLDTKDFQFLYAEGDDLVFMDVENYDQINLPKDLLGDA
                     AAFLQDGMTAVLEMYDERPISVQLPEQVEATIVEADAVVKGQTASSSYKPAVLDNGVR
                     ILVPPHIASGTRIVVNVYERTYVGKAD"
     gene            8292..8618
                     /locus_tag="VFF98_16285"
     CDS             8292..8618
                     /locus_tag="VFF98_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZU65247.1"
                     /translation="MVAAAPALADGPLGAVKRGDYVCELPGDAMGKPGIHQPAEDFSI
                     QQHSIYINAQGKGTYLLIGNRLTMTSGPKHGTLYRRVNDTFWRKLNADGSDNPLRCIR
                     SVLNNR"
     gene            complement(8615..10606)
                     /locus_tag="VFF98_16290"
     CDS             complement(8615..10606)
                     /locus_tag="VFF98_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZU65248.1"
                     /translation="MSETPSAPSAQVAAPRELTWRAIALGAAITVLFTAANVYLGLKI
                     GITFATSIPAAVISMAVLRHLPGVSIQENNIVQTIASAAGTLSAIIFVLPGLVMIGWW
                     RGFAFGPSVAIIALGGVLGVMLSVPLRRALLDDPALPFPEGVAAAEVLKLGDDSAGSE
                     DNARGLRAIVGGAAVSAGYALLAKLGVVAEEVSRFVRLGAGGTSLSASLSLALIGVGH
                     LVGLAVGLAMLAGLVIGWLVLLPHYTAMGPPPGVEWADFVATTFRHKVRFVGAGTMGV
                     AAIWTLLRVIGPVARGLSGALAAQRLRRAGQGHTLPLAERDVPIGLVGLAIALALVPV
                     GVLLAGFAGIGPLADGSGAPLAVHGALIVALALAYVLVAGAIIALVCGYMAGLIGSSS
                     SPVSGTGILTALGIALLLLAVVGAGADGATTRAAVAFALFVTSVIFGISTIANDNLQD
                     LKTGQLVGATPWRQQVALLLGVGFGAVVIPPLLDLLNHTFGFAGTPGAGPQALAAPQA
                     ALITAIIEGVLGGNLDWGLIGLGAGIGIAAILVDEALRAAGRGSLPPLALGMGIYLPM
                     GITLLTPLGALIGHAYHRFTARSADPARAERLGALAATGLIAGESLFGVGFAGLAAAT
                     GKPAPMALLTDFAGAAPLGLALCALLVGWLYRRTARAAR"
     gene            complement(10654..11322)
                     /gene="thiE"
                     /locus_tag="VFF98_16295"
     CDS             complement(10654..11322)
                     /gene="thiE"
                     /locus_tag="VFF98_16295"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZU65249.1"
                     /translation="MTETEAPQFEHVREATRIYLISPLEVGGSFPDRLARALDASAGD
                     AHGVAAFQFRVKDVEEHAAARLAEPLQRICADQGVAFIVNDSAKLAKRLGADGVHLGQ
                     DDDNAREARELLGRDAQIGVTCHASRHLAMEAGDAGADYVAFGAFFPTTTKEVKHHAE
                     PEILTWWQSLFELPCVAIGGINPANAAGLVRAGADFLAVSAAIWQGDEAANVRALAEA
                     IRAA"
     gene            complement(11495..12406)
                     /locus_tag="VFF98_16300"
     CDS             complement(11495..12406)
                     /locus_tag="VFF98_16300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZU65250.1"
                     /translation="MTTKIVQKILANYESDNPGVKANLHRILMQGKLGGTGKLIILPV
                     DQGFEHGPARSFSVNDPAYDPHYHYQLAIDAGLSAYAAPLGMLEAGADKFAGQIPTIL
                     KMNSSNSWATAANQAVTASVEDALRLGCSAIGFTIYPGSDDVFEMIEEIKELRAEAAA
                     VGIATVIWSYPRGGKLPKSGELALDVGAYAAHIAALIGAHIIKVKLPSDHIEQEDAKK
                     AYAGVDWSNQADRVNHVVKACFAGRRIVVFSGGAAKGADAVYQDARDIRDGGGNGSII
                     GRNTFQRPRDEALAMLDKLVKIYKGKE"
     gene            complement(12683..13882)
                     /locus_tag="VFF98_16305"
     CDS             complement(12683..13882)
                     /locus_tag="VFF98_16305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZU65251.1"
                     /translation="MAFRKLDDLGDVTGKVALVRVDFNLPMQDGAVTEDTRVRAVAPT
                     ILELADKGAKVLLLAHFGRPKGARAADQSLSLVLGAVEKVLGREVMFVPEIAGDVVAQ
                     AVGILRAGDIALLENSRFWPGEEKNDPEFARAIAANGDFYVNDAFSAAHRAHATTEGL
                     AHLLPAYAGRSMQAELEALEAALGAPVKPVAAVVGGAKVSSKLDVLKHLVTKVDHLII
                     GGGMANTFLAARGVDVGKSLCEHDLTGTAEEILEAADKAGCTVHLPYEVVVSKEFAAN
                     PPSLRTCNVHEVAADEMILDVGPAAVEALADALKTCRTLVWNGPMGAFETQPFDAATV
                     ALARIAAALTKEGSLVSVAGGGDTVAALHHAGVAADFTYISTAGGAFLEWMEGRELPG
                     VKALDIA"
     gene            complement(13971..14978)
                     /gene="gap"
                     /locus_tag="VFF98_16310"
     CDS             complement(13971..14978)
                     /gene="gap"
                     /locus_tag="VFF98_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZU65252.1"
                     /translation="MAVKVAINGFGRIGRNVARAILERPDCGLELVSINDLADAKANA
                     RLFKNDSVHGPFPGTVEVDGNDLILNGKRVQVTAEKDPANLPHAANGIDIALECTGFF
                     VDRDSASKHLAAGAKRVLISAPAKKVDRTVVFGVNHETLTAEDLIVSNASCTTNCLAP
                     FAKVLHEAIGIERGLMTTIHSYTNDQKILDQIHKDPRRARAAAMNMIPTSTGAAVAVG
                     EVLPDLKGKLDGSSIRVPTPNVSVVDLTFTPKRDTSIEEVNALLKAAAEGPLKGVLGY
                     TEEPLVSIDFNHDPHSSTIDALETAVIDGKLVRVLSWYDNEWGFSNRMLDTAGQMAKF
                     L"
     gene            complement(15041..>15727)
                     /locus_tag="VFF98_16315"
     CDS             complement(15041..>15727)
                     /locus_tag="VFF98_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZU65253.1"
                     /translation="LVFSDYCRNAIRMSALQQAKVVYVLTHDSIGLGEDGPTHQPIEH
                     VMSMRMIPNLEVFRPADVIETAECWQIAIANEGTPSVLALTRQNLPQLRHNGPNLSAR
                     GAYRLEAAAAPRKVVLVATGSEVEIALAVRAELEAQGIGADVVSMPSMSRFLAEDGAY
                     KADVLPAGVLKVSIEAGTTLGWQAITGLDGLNIGIDSFGASAPADQLYDHFGLTPAKI
                     TPRVLAALQG"
BASE COUNT         2652 a         5476 c         5094 g         2505 t
ORIGIN      
        1 ggtgaaggcg cccttctcca cgccgaacag ttccgcttcc agcaggcttt ccgggatggc
       61 cgcgcaattg accgccacga aaggcttttc cgcccgccgc cccatggcat gcagattgcg
      121 cgcgaagatc tccttgccca ccccgctttc gcccagaaac agcaccaccg catcggtatc
      181 ggccacatgg cgcagcaggt cgaaggccac gttgaagccc gccgaggcgc cgacgatgcg
      241 ccgctctgcc ttgccgtgaa acaccggcgg gtggcgctcc atctggttca gctgctcgcc
      301 gcgcatatag cgcagatccg cctcgccctc ctcccattct tccgccggac ggcccaccac
      361 gcggcagcag gcatggccca tggcaatgca ttccacctcg cggaaaagga tcggccggcc
      421 gaggaacacg ctggcatagc cgctggcata gccggtttgc gaccagccgg ccggatgcgc
      481 accgcgcccg aagatctcca catgggcggt gcattccgag gagcttttcc agagatattc
      541 ggcggcgaac tcgcccttgg cggtgtcgaa acgcacatcc accggctctg cgcgcaccat
      601 cccttccagc gtatgcagct gggggcccac ggcgaacatg tcgtcgatgg aagcggtggg
      661 gcgcaggcgg gcggcgatct cggcatccat cgcgcccgaa ttatagccct gccgggtgaa
      721 caggccgcgc gccttctcca gccccagcgc ctgcatcacc tcatagcgca acaggccgaa
      781 ggcgcgggcg tgcagcagca tcatccgccg cccggcgagc cagatatgcc cctgcgccgg
      841 atcgaaatgg agatgggaaa acagatcgtt ggccatggcg gtcctttcgc cggcgatgtg
      901 tagaatattc tgcacatcag ggcaaatgcc gcggtggata tgcagcatat tctacacacc
      961 ggatcccggc ggcccggcgc acgcgcaccc ctgattgcat aaaaaccatg cataaccagc
     1021 ttgttgtgct ttttcctggg gccccggcgg catttggcac ggtgctggca atgcacttct
     1081 ccgaaggccg cgcgaacacc ggccgccaga gaggagattg cctgaccgcc agcggccttt
     1141 gcgccgacta gccccaaaag cggcgcccgc gccgtttcgc gccgcaacgg gcgccatcac
     1201 cacaccacca cacccgcgcc cacgcgcgcc aaaccataat tgccagagga gaaaccggat
     1261 ggagctgcct ttccagcccc tcactcgccg cacattcctg tctgccagcg cgctggccag
     1321 cgccgccatc gccgcgcctg ccgccgccat ggcgctgaac gcccggccgc tggcgctggc
     1381 ctccagccac caggccctgc cggccagctg gcaaggcccg gcgctggtgc tctcgggcga
     1441 ttatctcgcc cggcttggcc agttgcgcca ggcgctggaa agccacgcgc cggccagctg
     1501 ccgcctgttt ctcgacgatg ccgaccgcgt gctgttcgac gtcgccgccc atgcccccca
     1561 tgccaaagga tttgccgcat gatccgccgc cctgtttccc gcgccgcctc ccgccccgcc
     1621 gcccgcgtgg cctcgctggc ggccggcgcc ctgggccttg gcggcgtgct ggccgccgtg
     1681 ctggccggcc ctgccgccac gcccgcccag gcctcgccca ccggcgtgtg gaccagcccg
     1741 caggaagcct atgacaagat ctgcgcgcgc tgccacctct cgggcgtggg gccggaactg
     1801 cgcgggcgcg cgctgccgcc cgaatatatc aagctgatcg tgcgttcggg ccgcctggcg
     1861 atgcccgcct tcccccattc ctcgatcgac gatgccaccc ttgatggtgt ggcccgcctt
     1921 gtctcggcgc agaagctgcc caagcccgcc gctgccccca agcgctgaac cggagagttc
     1981 ccatgaccga ccagaccacc ccggccgtgc tgccccatgg cgtgagcacc gccgatttcc
     2041 gcgccgccat tgccgaattc atcgccattc tgggcgcggc caatgtgatg accgacgagg
     2101 cccacatcgc cccctacacc aaggtgatgg tggccgagag cgaggatctg caccgcccct
     2161 cggcgatcct ctatgcccgc gaagtgggcg agatccagaa gatcctcgcc gtttgcaaca
     2221 catacaaggt gccgatctgg acgatctcca ccggccgcaa tttcggctat ggctcggcag
     2281 cgccgcaaag cgcaggccaa gtggtgctgg atctgcgcca catgaacaag atcatcgagg
     2341 tggacccggt gctgtgcacc gcgctggtgg agcccggcgt tacctaccag cagctgaagg
     2401 actatctggt cgagcataac atcccgctgt ggctgtcgtg cccggcgcct tcggccattg
     2461 ccgggccttt gggcaacacg gtggatcgcg gcgtgggcta cacgccctat ggcgagcatt
     2521 tcatgatgca gtgcggcatg gaagtggtgc tggccaatgg cgaggtgctg cgcaccggca
     2581 tgggcggggt ggaaggcacc agcgcctggc aggtgttcaa atggggctat gggccctatc
     2641 tcgatggcat cttcacccag tcgaactatg gcatcgtcac caagatgggc atgtggctga
     2701 tgccgcagcc gccggtctac aagcccttct gcatccgcta cgagcatgac gaggacatcc
     2761 acgatatcgt ggaaacgctg cgcccgctgc gcatcgccaa tgtcatcccc aatgccatgg
     2821 tgtttgccaa tgtgatgtgg gaagcggccg ccatgatgcc gcgcagcaaa tattacagcg
     2881 gcaccggcac cacccccgat cacgtgctgg acgagatcaa ggccaaggaa gggctgggcg
     2941 cgtggaatgt ctatgccgcg ctttatggca ccaaggagca ggtggaggtc aactgggcga
     3001 tcatcaccgg cgccatcaag gccagcggca agggccggat catcaccgag gaagaggccg
     3061 gcgagaccga gcccttcaaa tatcgcgcca agctgatgcg cggcgagatg accctgcagg
     3121 aatttggcct ctatcgctgg cgcggcggcg gcggctccat gtggtttgcc ccggtgacgg
     3181 cggccaaggg cagcgagacg gtggagcaga cgcgcctcgc caagcagatc ctgggcgaat
     3241 atggccttga ttacgtggct gaatatatcg tcggcatgcg cgatatgcac catatcgtcg
     3301 atgtgctttt cgaccgttcc gacccggaag agatgaagcg cgcgcatgac tgcttcgcca
     3361 agctgctgag cgagttcggc aagcgcggct atgcggtcta ccgcgtcaac accgccttca
     3421 tggaccagac cgccgacctc tacggcccgg tgaagcgcaa ggtggaccag acgctgaagc
     3481 gcgcgctcga ccccaacggc attctggcgc ccggcaaatc gggcatccgc atctgatcct
     3541 tccccctttt gcagaacagg acttttcatg cctcatctga ttggccgcgc atttggcgcg
     3601 gccctgctgc cgcttgccct tgccgccacc cccgccgccg cccagagcgg cgatgccacc
     3661 cgcggccgcg ccgtgttcca ggggcagtgc ggcgtgtgcc attcggtggc gccgggcaag
     3721 gcgatcatcg gccccagcct tgccggcatt gtcggccggg cggcgggcaa gcaggccggc
     3781 tttgcctatt cgcccgccct cgccaaggcc ggcttcaagt gggacaaggc aaagcttgat
     3841 gcctggatcg ccgggcccgc caaggtggtg ccgggcaacc gcatgccctt tgccggcctg
     3901 gccgatggcg ccaaacgggc cgatctcatc gcctatctgg ccaaccccaa gtgagcgggg
     3961 gcggcctgcc tgtggcgctg ctgatcgacg gggcagaccg ccccgccagc catggcgcca
     4021 ccttcacccg ccacgggccg ctgggcgatg gcccggccag cacggcagcg gccgccagcg
     4081 tggaggatgc agcagcggcg gcagaagcgg cgcaggccgc cctgccggcc tgggcggcgc
     4141 tgggccccaa tgcccgccgc cgcctgctgc tggccgccgc agacgcgatg gaagcgcgcg
     4201 cgggcgattt cgtcgcggcg atggcggcgg aaacgggcgc ctctgccggc tgggcgggct
     4261 tcaacgtgca tctgggcgcc agcctgctgc gcgaggccgc cgcgctcacc acgcaggtga
     4321 agggcgagac catcccctcc gaccgccccg gctgccacgc gatgaccctg cgccgcccgg
     4381 ccggcgtggt gctgggcatc gcgccgtgga acgcgccggt gatcctgggc gtgcgggcca
     4441 ttgccacgcc gctggcctgc ggcaatacgg tggtgctgaa agcctctgaa cattgccccg
     4501 ccacccaccg gctgatcggc accgtgctgc aggaagcggg ctttccggcg ggcgttgtca
     4561 acgtgctcag caatgcgccc gaggatgccg gcgccgtggt ggcagcgctg gtgcgccaca
     4621 aggcggtgcg gcggatcaac ttcaccggct ccacccgggt ggggcggatc atcgccgggc
     4681 tggccgcgca ggagctgaag ccctgccttc tcgaactggg cggcaaggcg ccgctgatcg
     4741 tgctggacga tgccgatgtg gaggcggccg ttgccgccgc cggtttcggc gccttcatga
     4801 acagcgggca gatctgcatg tcgaccgagc ggatcatcct gcagggcggc attgccgatg
     4861 cctttgtcgc gctgttccgc cagaaggtcg ccagcctcca tgccgccgat ccgcgccagg
     4921 gggcagcccc gctcggctcg gtggtggatg aggcaacggc cgatcgcatc cgccacctca
     4981 ttgccgatgc gcgcgcgaag ggcgcggtgg tctatggcga ggtgccggca accggcacgc
     5041 tgctaccagc catcgcgctc gaccatgtga cgccggaaat ggcgatctat gccgaggaaa
     5101 gcttcggccc ggtcaccacc attctgcgcg cggccgacga cgaggaagcg ctgcgcctgg
     5161 ccaacgacac cgaatatggc ctcacctcgg cggtgttcgg gcgggatctg gcgcgtgccc
     5221 tgtcggttgc cgagcggatc gaggcgggca tgtgccacat caacggcgcc accgtgcatg
     5281 acgaggcgca gatgcccttc ggcggcacca aggcctctgg ctacggccgc tttggcggcg
     5341 aggcggggat cgccgaattc accgagctga agtggctgac gatcgacacc ggcaccccgc
     5401 actggccgat ctgacaggca gcgggcggcc ccgcttaacc ggcggcgccc gcgccaaccc
     5461 taataatcga cacaagggag aggaacacat gataaaaaca cgcaagctgg cgctggccat
     5521 ggccctttcg gctgctgcca tcgcccatcc ggccgccgcc agcgagagcg gcggcggcgt
     5581 ttatccggtg ggcgcggaag gggcgctggg cggcgcgctg ccgccgccgg gggtctatct
     5641 gctgggctat ctgcaatcct atgaggcggg gcgcttcaac gatggcaagg gcaatgccgg
     5701 cttcctgccc gatttccacg tgagcgcaga agcggcggtg acgcgcgtgg tgtggaacac
     5761 cggccgcaag ttcctgggcg ccgattatgc catgcatctg gtggcgcccg cggtggtgat
     5821 cgatgcccgg gtggccggcg tttccgaccg gcgggccggc ttcaccgatg tcaccatcga
     5881 tcccttcatc ctgggctggc actggaagag cggcttccac atcatcaccg gcctcgacat
     5941 caacctgccg gtgggcagct acagccgcac cagccccgcc aacctcagcc gccactactg
     6001 gaatgtggag ccgatcatcg cggcggccta ttatggcccc cacggcctgc aggtggacat
     6061 gaaggcgatg tatgacatca acttcaccaa ccacgatgcc cagatcaacg ggctcaaccc
     6121 caccggatcg ccctatcgtt ccggcaatga attccacatg gaatatgccg tgaccaaggc
     6181 gctggccccg cgcctcagcg ccggcatcgc cggctactac taccgccaga ccacgcgcga
     6241 ccatgtggac aacaccgatg cccagcaatt gctcgacagc tttggcgggc tgaagggcga
     6301 ggccgtggcc gccggcccca ccctgcgcct caccgccggc aaggcgcaga tcatcgccac
     6361 ctggcagcat gaattcgacg ccgcctatcg cccccagggc gacaaggtgt ggctgaaggc
     6421 gatcatcccc ctggcagggg ttggcaagcg ctgatcagcc agaagcaacg ctgaaaggca
     6481 acagggcccg gcagctccac caaggagctg ccgggccctg ttttgctatc ggccagattg
     6541 gccaagggat ggtacgggcg gccggactcg aaccggcatg ttgttgccaa cggcggattt
     6601 tgaatccgct gcgtctacca ttccgccacg cccgcacttt gtgcccttgc cggaacacac
     6661 cgcctagcgc cgggccgcgg caggatcaag ccctgccagc gtgttttggc ccgcctgttt
     6721 tgccccgcct gtttgactgc cccccggccc tgcctcagtc cctcagggcg ccggccagga
     6781 tcttgtgcag gcgcgaatgc agcgcatcat tgcctgccag cacctcggca tcgcagatcg
     6841 gcagcgactg gcccttccag ttcgacacga agccgccggc ctcgcgcacc agcaggcagc
     6901 cggccgccgt gtcccagggc ttcaggctcg attcccagaa gccctcgtag cggcccgcgg
     6961 ccacccaggc gagatcgagc gcggccgcgc caaagcggcg gatgcccgcc acctgcggcg
     7021 ccagcgcctg atagatgcgg ctccattcca ccacatcgcc atggccggca aacggaatgc
     7081 cggtggcgat caggctttca tcgagatggc gccggcccga cacgcgcagg cggcgatcat
     7141 gcagccaggc gccgcggctc ttctcggccc agaagctctc gtcggtgatc ggctggtaga
     7201 ccaggccggc caccacctcg ccccagccct cgccgcccgg cagcggttcc tgcgcggcga
     7261 tggagatggc gaaatgcggg atgccatgga ggaaattgct ggtgccatcg agcgggtcga
     7321 tgatccagcg cggcttggtg gggtcaccct ccaccgtgcc gccttcctcc atcacgaagc
     7381 cccagtcggg gcgggcggcg cgcagttcct catagagggt gcgctcgctc gcctggtcgg
     7441 cgcggctgac gaaatccgcc ggccccttgc gcgagacctg caggtgctcc acctcgccga
     7501 aatcgcgacg cagccgctgc ccggccttgc gggcagcgcg ctccatcacg cggatcatgc
     7561 cggaaatggc catggccggt cagtccgcct tgccgacgta ggtgcgctcg tagacgttca
     7621 cgacaatgcg cgtgcccgag gcgatgtgcg gcggcaccag gatgcgcacg ccattgtcca
     7681 gcacggcggg cttgtaggac gaggaggcgg tctgcccctt caccacggca tcggcttcca
     7741 cgatggtggc ttccacctgc tcgggcagct gcaccgagat cggccgttca tcgtacattt
     7801 caaggacagc ggtcataccg tcctgcagga aagcggcggc atcgcccagc aggtccttgg
     7861 ggaggttgat ctggtcgtag ttctcgacat ccatgaacac caggtcatcg ccctccgcat
     7921 agaggaactg gaaatccttg gtgtcgaggc gcacgcgctc caccgtgtcg gcgctgcgga
     7981 aacgcacatt ggtcttgcgt ccatcgatca ggttcttcat ttcgacctgc atgaaggcgc
     8041 cgcccttgcc gggctgggta tgctgggtct tggcgacctt ccagatgcca ccttcatatt
     8101 caaggatgtt gccggggcga atttcgacgc cgctgatctt catgggaatg gaaccttcga
     8161 tgataaagag ccgtttgacc gcagctgccg cagccgccac gcatatggcc gccggcctta
     8221 gcgtgcgcaa tggcatctgg caatctcggc tttgcgccgg gatggccgca gttggcacag
     8281 ccgcaagcct catggttgcc gccgcaccgg cccttgccga tggcccgctg ggcgctgtca
     8341 agcggggcga ttatgtgtgc gaattgcccg gcgatgccat gggcaagccc ggcatccacc
     8401 agccggccga ggatttctcg atccagcagc attccatcta tatcaatgcg cagggcaagg
     8461 gcacctatct gctgatcggc aaccggctga cgatgaccag cgggcccaag cacggcaccc
     8521 tctatcgccg ggtgaacgac accttctggc gcaagctcaa cgccgatggc agcgataacc
     8581 cgctgcgctg catccgcagc gtgctgaaca accgctagcg cgccgcccgc gccgtgcgcc
     8641 ggtagagcca gcctaccagc agcgcgcaca gcgccagccc caaaggcgca gcgccggcaa
     8701 aatccgttag cagggccatg ggggcgggct tgccggtggc cgcggcaagc ccggcaaagc
     8761 ccacgccaaa caggctttcc ccagcgatga ggccggtggc cgccagcgcg cccagccgct
     8821 cggcccgggc gggatcggcg ctgcgggcgg tgaagcggtg ataggcatgg ccgatcagcg
     8881 cgcccagcgg ggtgagcagg gtgatgccca tcggcaggta gatgcccatg cccagtgcca
     8941 gcggcggcag gctgccccgg ccggcggcgc gcagggcctc gtccaccagg atggcggcga
     9001 tgccgatgcc tgcgccaagg ccgatcagcc cccaatcgag attgccgccc agcaccccct
     9061 cgatgatcgc ggtgatgagg gcggcctgcg gggcggccag cgcctgcggc ccggcgccgg
     9121 gcgtgccggc aaagccgaaa gtgtgattca acagatcgag cagcggcggg atcaccaccg
     9181 cgccaaaacc cacccccagc agcagcgcca cctgctggcg ccatggcgtg gcgcccacca
     9241 gctggccggt cttcagatcc tgcagattgt cgttggcgat ggtggaaatg ccgaagatca
     9301 cgctggtgac gaacagcgca aaggcgaccg cggcgcgggt ggtggcgcca tctgccccgg
     9361 cgcccaccac cgccagcagc aacagggcaa tgcccagcgc ggtgaggatg ccggtgcccg
     9421 agaccgggct gctggaagag ccgatgagcc cggccatata gccgcagacc agcgcgatga
     9481 tggcgcccgc caccagcaca taggccagcg ccagcgccac gatcagcgcc ccatgcacgg
     9541 ccagcggcgc gccagaacca tcggccagcg gcccgatgcc ggcaaagccc gccagcaaca
     9601 cccccaccgg caccagcgcc agggcaatcg ccaggccaac caggccgatc ggcacatcgc
     9661 gctcggccag cggcagggtg tggccctgcc ccgcccggcg caaacgctgc gccgccagcg
     9721 cgcccgacag cccccgcgcc accgggccga tcacccgcag caaggtccag atcgccgcca
     9781 cgcccatggt gcccgcgccc acaaagcgca ccttgtggcg gaaggtggtg gccacgaaat
     9841 cggcccattc cacccccggc ggcgggccca tcgcggtgta gtgcggcagc agcaccagcc
     9901 agccgatcac cagcccggcc agcatcgcca gccccaccgc cagccccacc agatggccca
     9961 cgccaatcag cgccagcgac aggctggccg aaaggctggt gccccctgcc cccagccgca
    10021 cgaagcgcga aacctcctcg gccaccacgc ccagcttggc caacagcgca tagccggccg
    10081 ataccgcagc ccccccgaca atcgcgcgca gcccccgggc gttgtcttcg ctgccggcgc
    10141 tgtcatcgcc cagcttcagc acttcggccg cggccacgcc ttccggaaag ggcagcgccg
    10201 gatcatccag cagcgcccgg cgcagcggca ccgacagcat cacccccagc accccgccca
    10261 gcgcgatgat cgccacgctg gggccaaagg caaagccccg ccaccagccg atcatcacca
    10321 gccccggcag cacgaagatg atcgccgaaa gcgtgccggc ggcactggcg atcgtctgca
    10381 cgatgttgtt ttcctgaatg gaaacgcccg gcaaatggcg cagcaccgcc atcgagatca
    10441 ccgccgccgg gatcgatgtg gcaaaggtga tgccgatctt gaggccaaga tagacattgg
    10501 ccgcggtgaa cagcacggtg atcgccgcgc caagggcaat ggcgcgccag gtcagttcac
    10561 gcggggcagc gacttgggca gaaggggcag aaggggtttc gctcatggcg gcgggtgtgg
    10621 catggccgcg tcccgccgca aagccctgat ctgtcaggcc gcgcggatcg cctcggccag
    10681 ggcgcgcacg ttggcggcct catcgccctg ccagatcgcc gccgagacgg cgaggaaatc
    10741 ggcgcccgcc cgcaccagcc ctgccgcatt ggcagggttg atgccgccga tcgccacgca
    10801 gggcagttcg aacagcgatt gccaccaggt gaggatctcg ggctcggcgt ggtgcttcac
    10861 ttccttggtg gtggtgggga agaaagcgcc gaaagcgaca taatccgccc ccgcatcgcc
    10921 cgcttccatg gccagatggc ggctggcgtg gcaggtcaca ccgatctgcg cgtcgcggcc
    10981 cagcagctcg cgcgcctcgc gcgcattgtc atcgtcctgg cccagatgca cgccatcggc
    11041 gcccagccgc ttggccagct tcgccgaatc attgacgatg aaggccacgc cctgatcggc
    11101 gcagatgcgc tgcaggggtt cggccaggcg ggcggcggca tgctcctcca catccttcac
    11161 gcggaactgg aaggcggcaa cgccatgcgc atccccggcc gaggcatcga gcgcgcgcgc
    11221 cagccggtcg gggaagctgc cccccacctc cagcggcgag atcagataga tccgggtggc
    11281 ttcgcgtaca tgctcgaatt ggggggcttc ggtctctgtc atggcgcgag ccttagaaca
    11341 tcgggccggc aggggggaac cggttttttc gccccggcgc ccgcgcccac gaaaaagcgc
    11401 ggcgaaggcc ttgtcagccc cgccgcgcct tgttcaccag cacgggcctt gcggccctgc
    11461 ctttgccgtt gcgggccttg cggcccgggt gggcttattc cttgcccttg tagatcttca
    11521 ccagcttgtc gagcatggcg agcgcttcgt cacgcgggcg ctggaaggtg ttgcggccga
    11581 tgatcgagcc attgccgccg ccatcgcgga tgtcgcgcgc gtcctggtag acggcatcgg
    11641 cgcccttggc cgcgccgccc gagaacacca cgatgcgccg gccggcaaag caggccttga
    11701 cgacatggtt cacccggtcc gcctggttcg accagtcaac gccggcatag gccttcttgg
    11761 cgtcttcctg ctcgatatgg tcggaaggca gcttcacctt gatgatgtgc gcgccgatga
    11821 gggcggcgat atgggcggca taggcaccca cgtccagcgc cagctcgccg ctcttgggca
    11881 gcttgccgcc gcgcggatag gaccagatca ccgtggcgat gccaacggca gccgcctcgg
    11941 cgcgcagttc cttgatctcc tcgatcatct cgaagacatc gtcggaaccg gggtagatgg
    12001 tgaagccgat ggccgagcag cccaggcgca gcgcgtcttc cacgctggcg gtcacggcct
    12061 ggttggcggc ggtggcccag ctgttcgagc tgttcatctt gaggatggtg gggatctgcc
    12121 cggcgaactt gtcggcgccc gcttccagca tgcccagcgg cgcggcatag gccgacaggc
    12181 ccgcatcgat cgccagctgg tagtggtagt gcggatcata ggccggatcg ttcaccgaga
    12241 agctgcgggc cgggccatgt tcgaagccct ggtccaccgg caggatgatc agcttgcccg
    12301 tgccgcccag cttgccctgc atgaggatgc ggtgcaggtt cgccttcacg cccggattgt
    12361 cggactcgta attggcaagg atcttctgga cgatcttggt ggtcatgttg tccctctgtc
    12421 tgccgcagcc aaactgcatc tgccgagttg gcgcataggt atgcaccggg catggcctta
    12481 gcggccctgc tggcgcatcg caacccgcct gaagcgtcat ttttacgcat aaggagcggt
    12541 ttttcagctg aaaaacagcc caaactgcgc aaaatgcgct tctgccggcc tgcttatggc
    12601 ctcttcacgc gaaagtaaaa tgacaaaggc cgggcgcgcc ggcccggcct tcctatcatg
    12661 cccgaaaaca ggccctgccc gctcaggcga tatcgagcgc cttgacgccg ggcagttcgc
    12721 gcccttccat ccattcgagg aaggcgccgc cagcggtcga gatataggtg aagtctgccg
    12781 ccacgccggc gtggtgcagc gctgccaccg tatcgccgcc gccggccacg ctgaccagcg
    12841 agccttcctt cgtcagcgcc gccgcaatgc gcgccagcgc caccgtggcg gcatcgaagg
    12901 gctgggtttc aaaggcgccc atcggcccgt tccacaccag cgtgcggcag gtcttcagcg
    12961 catcggccag cgcttccaca gcggccgggc ccacatcgag gatcatctcg tcggcggcca
    13021 cctcatgcac gttgcaggtg cgcaagctgg gcggattggc ggcaaattcc ttcgacacca
    13081 ccacctcata gggcagatgc acggtgcagc cggccttgtc ggccgcctcg aggatctcct
    13141 cggcggtgcc ggtgaggtca tgctcgcaca ggctcttgcc cacatccacg ccgcgcgcgg
    13201 ccaggaaggt gttggccatg ccgccgccga tgatcaggtg atcaaccttg gtgacgagat
    13261 gcttgagcac atcgagcttg ctcgacacct tggcgccgcc gaccaccgct gccaccggct
    13321 tcaccggcgc gcccagcgcc gcttccagcg cttccagctc tgcctgcatg ctgcggccgg
    13381 cataggcggg cagcagatgg gccaggcctt ccgtggtggc atgggcgcga tgcgcggccg
    13441 agaaggcatc gttcacatag aaatcgccgt tggcggcaat ggccctggca aattccggat
    13501 cgttcttttc ctcgccgggc cagaagcggc tgttttccag cagggcaata tcgcccgcgc
    13561 gcaggatgcc caccgcctgg gccaccacat cgccggcgat ctcgggcacg aacatcactt
    13621 cgcggcccag caccttttcc accgcgccca gcaccaggct cagcgactga tcagccgcgc
    13681 gcgcgccctt ggggcggccg aaatgggcca gcagcagcac tttggcgccc ttgtcggcca
    13741 gttcgaggat ggtgggggcc accgcgcgca cgcgggtgtc ttccgtcacc gcgccatcct
    13801 gcatcggcag gttgaaatcc acccgcacca gcgcgacctt gccagtgaca tcgcccagat
    13861 catcgagctt gcgaaacgcc ataaaacccc ctgcccccgc cttgcctgtc gcggggctca
    13921 ttcaaacagc acagcgaaaa agggcggggt tttcaccccg ccctgttcga tcaaaggaac
    13981 ttggccatct gcccggcggt gtcgagcata cggttggaga agccccactc gttgtcgtac
    14041 cagctgagca cgcgcaccag cttgccgtcg atcaccgccg tctccagagc gtcgatggtc
    14101 gacgaatgcg gatcgtggtt gaagtcgatc gagaccagcg gctcctcggt gtagcccagc
    14161 acgcccttca gcgggccttc ggcagccgcc ttcagcagcg cgttcacttc ctcaatcgag
    14221 gtgtcgcgct tgggggtgaa ggtgagatcg accaccgaga cgttcggggt cggcacgcgg
    14281 atcgaggaac cgtcgagctt gcccttgagg tccggcagca cttcgcccac ggccacggca
    14341 gcgccggtgc tggtggggat catgttcatc gcggcagcgc gggcgcggcg cggatccttg
    14401 tggatctggt cgaggatctt ctggtcgttg gtgtagctgt ggatcgtcgt catcaggccg
    14461 cgctcgatgc cgatcgcctc atgcagcacc ttggcgaagg gggccaggca gttggtggtg
    14521 cagctggcgt tggagacgat caggtcctcg gcggtcagcg tctcgtggtt gacgccgaac
    14581 accacggtgc gatccacctt cttggcgggc gccgagatca gcacgcgctt ggcgccggcg
    14641 gccaggtgct tgctggccga atcgcgatcg acgaagaagc cggtgcattc cagcgcaatg
    14701 tcgatgccat tggcggcgtg gggcaggttc gccgggtcct tctcggcggt cacctgaacg
    14761 cgcttgccgt tgaggatgag gtcgttgcca tccacttcca cggtgccggg gaagggcccg
    14821 tgcacgctgt cgttcttgaa caggcgggca ttggccttgg catcggccag atcgttgatc
    14881 gagaccagct caagcccgca atcggggcgc tccagaatgg cgcgggccac attgcgcccg
    14941 atacgaccga aaccgttgat cgcaaccttc acagccatgg aacttgtcct cctttataag
    15001 ccttgggcgc cgcaccctgg cgcggcgtgt ttgtgccggt tcagccctgc agcgcggcca
    15061 gaacgcgcgg cgtgatcttg gcgggggtca gcccgaaatg atcgtagagc tggtcggccg
    15121 gcgccgaggc gccgaagctg tcgataccga tgttgaggcc atcgagcccg gtgatcgcct
    15181 gccagccgag cgtggtgccc gcctcgatcg agaccttgag cacgcctgcg ggcagcacat
    15241 cggccttgta ggcgccatcc tcagcaagga agcggctcat cgagggcatg gagacgacat
    15301 cggcgccaat gccctgtgct tccagttcgg cgcgcacggc aagggcgatc tcgacctcgc
    15361 tgccggtggc gaccagcacc accttgcggg gggccgcggc cgcttccagc cgataggcgc
    15421 ccctggccga gaggttcggg ccattatggc gcagctgcgg caggttctgg cgggtcagcg
    15481 ccagcacgct gggggtgccc tcattggcaa tggcgatctg ccagcattcg gccgtctcga
    15541 tcacgtctgc cgggcggaac acttccagat tggggatcat gcgcatgctc atcacatgct
    15601 cgatcggctg gtgggtgggg ccatcctcac ccaggccaat gctgtcatgc gtcagcacat
    15661 agacgacctt ggcctgctgc agcgccgaca tgcggatggc attgcggcaa taatccgaga
    15721 acaccag
//
LOCUS       DASRYE010000317        38423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_264579,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000317 DASRYE010000000
VERSION     DASRYE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 38423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38423
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264579"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFF98_16320"
     CDS             complement(<1..818)
                     /locus_tag="VFF98_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZU65254.1"
                     /translation="MGRAGLLVAGALALGGAAPAPKGPAPLRPAPLRIAFLAAGAQNG
                     FNQSVWQGIRQAAARQRGVEVTLYDGAFDSTLQYNQLEDVIASHRYDGIILFPNDSVS
                     IASAVRHAGAVGIRTVAVEFPIGPQLDELAPQVPGVVATVAARPSDGARAQAQDVVAY
                     CAGRPQCNVVLVVGQKVYPFDLLRYRTYLATFAPHRNIRVVATIEGNYDAGTAMSGML
                     DVLQVNRRIDAVVSTADIQTIGVEMALKSYGIRPASVHLVGSGGSQIAVTALRQG"
     gene            971..1477
                     /locus_tag="VFF98_16325"
     CDS             971..1477
                     /locus_tag="VFF98_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZU65255.1"
                     /translation="MHPDMDSPTLATLTGLIAAPGQPDALLTRMARMLMADPGYAMLT
                     ILLYDVAGGRGRRIYSTDEVAYPTGAFKPIPMTDWADQVLVRHQPFIADTVADFRPHY
                     GDWAKLEAMGLISGLNTPIIVDGVAIGSVNFTARSEGYYTPARVEAARRCGALAAIAL
                     LLEARGAA"
     gene            complement(1511..2707)
                     /locus_tag="VFF98_16330"
     CDS             complement(1511..2707)
                     /locus_tag="VFF98_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZU65256.1"
                     /translation="MTEAIGGSNAASELADLKPWADVAPLITPAERAARLDKLRLHMA
                     AAGIDMALVDAGASLRYFAGVPWGQTERLVAMLVPLEGDPVLICPTFELGSLAADLAI
                     AASVRSWEEEENPFALVADVLRERQVARLGIDPEFPFGMLGRLRAVAPGVEVVDLAGP
                     INACRMIKSAAELAIMRQAKQMTLEVHRRAARIMRAGITASEVRRFIEAAHRALGAPG
                     NSFCIVEFGRSSAFPHGLPGDAVLAEGDIVLIDTGCPVQGYSSDITRTYVFGEADAFQ
                     REIWALERAAQQAAFDAVRPGVLAEEIDAAARKVLEGAGLGPDYRLPGLPHRTGHGIG
                     LSIHEPAYLVRGDKTPLAPGMCFSNEPMIVIPDRFGVRLEDHFHVTENGAEWFTRPAA
                     SIDDPI"
     gene            complement(2721..4001)
                     /locus_tag="VFF98_16335"
     CDS             complement(2721..4001)
                     /locus_tag="VFF98_16335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZU65257.1"
                     /translation="MGSVKSESRRILVIGGGIVGSATALALLGRGHAVTLVDEEGPRR
                     RASWGNAGHIAIEQVEPLASLATVMGVPRQLLARDGALALPWREAGRWLPFVARLAWA
                     ARPSAFARGQAALSGLLARAMPAWQRYVEALGAPALLRRTGHIIVWDRPESAARGRQA
                     WQADVPEGIRFRDLTADEEAQIAALRGGKAPAGALYCEGSGSIAGHDLLEAALRARFA
                     ALGGRWLQGRARLWAEGATVLAALADGTPLLADEIIVAGGVLSRPLLEGLGFAVPMIA
                     ERGYHLQAPGARWPADLPPVVFEDRAMIVTRFEDSVRASSFVELARPGAPADPAKWAA
                     LRRRISELGLPFELPGAEWLGARPTLPDYLPAIGRSRHHTNLLYAFGHQHLGLTLGPL
                     SGEIIADLVEGRAPPVSLAPFDIERFGARRGAST"
     gene            4049..4969
                     /locus_tag="VFF98_16340"
     CDS             4049..4969
                     /locus_tag="VFF98_16340"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZU65258.1"
                     /translation="MAELNWKGVYPAATTQFAPDLSVDVAASQQVLDGLIRDGVNGLI
                     LLGTCGENNSLEADEKHTLLEAAREVVAGRVPTVAGVSELTTARAVAFAKTAEQAGVD
                     GLMLLPAMVYVPTEEELVAHFKAVAAATSLPIMLYNNPPAYRTCVTEGALRQLLEVEN
                     IVAVKESAPDPRRFTDLHNAFGERFVLMAGLDDVALEGLMLGASGWVSGLTSAFPQES
                     VALVAAFERGDYDTARRIYRWFMPLLHLDAEHDLVQSIKLAEQIMGRGCERVRLPRLP
                     LAGARRAQVIAMVEKAAATRPGLAPLDAVA"
     gene            4971..5969
                     /locus_tag="VFF98_16345"
     CDS             4971..5969
                     /locus_tag="VFF98_16345"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HZU65259.1"
                     /translation="MRHTFFCIDGHTAGNPVRLVAGGAPQVQGNSMAERRADFLARFD
                     WIRTGLCFEPRGHDMMSGGFLYPPTRPDTDIGILFIETSGCLPMCGHGTIGMITFGLE
                     HGLIVPRTPGRLKVEVPAGLIDIDYGEEAGKVTFVRIRNVPSYLARAGIAIEVPGFGP
                     LLVDVAYGGNFYAIIEPQGPYRGLDDLGAARLIELSRSVRTLVQAVYEPVHPQDARIR
                     GVSHVQWADRPSAPDADGRNAVFYGEKAIDRSPCGTGTSARMAHLFATGRLQVGDRFI
                     HQSYIGSRFTGRVEATADVGGLPGIIPSIEGAAVATGHNTIWIDREDPFWAGFTVV"
     gene            6021..6680
                     /locus_tag="VFF98_16350"
     CDS             6021..6680
                     /locus_tag="VFF98_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZU65260.1"
                     /translation="MSIVIRSLSDQVFDVLRERILAGQVPTSSPIRQDALAAELGISK
                     IPLREALARLEQEGLLESQTNRGFFVRPMSTRELEEVYALRLKLEPDAVVAGARAATP
                     AQQEGARKALDDFKREADLRRISGGAHNRAFHVALIEPSGNRVTNDFLVRLHILADRY
                     VCKHLEPLGRNERADKEHDELLGAWLDQDEPRLRQLVYDHIFNTLADLREQLAAEGVS
                     G"
     gene            complement(6677..7972)
                     /locus_tag="VFF98_16355"
     CDS             complement(6677..7972)
                     /locus_tag="VFF98_16355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HZU65261.1"
                     /translation="MAGVNDLAGLKARLEEATAAALPRQTAWLADLVRFASTRGQEGD
                     CQRWLAGQFAGRGWQVDLFSLADVALAGAAGASPAVEVDLAEALQVVAKVPARVTEGR
                     SLILQGHIDVVPPGPRELWRHDPFDPQIADGRIHGRGTADMKVGVAAMVFALEALAAI
                     GLEPTADIFVETVSEEECTGNGALGTLLRGHVAEACLIPEAVGNRLLRAQLGSVWFRL
                     RLLGEPAHILERGPGASAILQMYDHACALQDLAAAANRLAAEDPWFGAMAQPVTFSMG
                     KICGGDWIGMVPAWCEADCRIGVLPGQSVAAVREAICAQVAQCAARIGSAVPEVSWIG
                     FAADPYVLAPGGAAEAALASAHELVFGAPLESFCLPATTDVRQYGVHYGIPALCYGSS
                     GGGIHAANEYADLESMRQTTITLALFVALWCGVRPALSA"
     gene            complement(7972..9630)
                     /locus_tag="VFF98_16360"
     CDS             complement(7972..9630)
                     /locus_tag="VFF98_16360"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZU65262.1"
                     /translation="MATSPHDAFTFDIIQNGLQAAADEMFAVQRKTSMSAVIYEVLDL
                     GTGICDAQGQIAASGAGIPAFVGVLDKAVLRIVEKHPAETLRPGDVFASNDPYCGGVT
                     HLNDMIIAMPVFAEGRLIAWSANIAHYNDVGGSVPGSMSTEATEIFQEGLRVPAVKLI
                     AGGVPNQAVFDILETNTRLPAFLRGDLWAGIAGVRIGERRLLEMATKYGVDTFMAAMA
                     SFMELGERRARAALARIPKGRFTFAEEQDNGAIHKIAIEITDEAFIIDVRDNPDQAGS
                     SNSSREGTEIAAQLAFKAVTDTEAPGNAGFFRPLQVLTRPGSVYHVQEPGALGYYAEV
                     EIRLFDLILRCLAAQFPHLVPAGNFSSICGTNIGGKHAETGEHYTVVEPQLGGWGAED
                     GRDGNNAIFSGFHGETFNCPAEVAEARYGLGVEEMALNITQAGAGQWRGGKGLSLRYR
                     VRADENFLSVGYTRSRIPPWGINGGADGSPNYVEVLRAGKAPERHAFGTNIPLGKGDV
                     VHVVTGVGGGCGDPHARDPEAVRRDIRDGYLTAEEARAIYGYAG"
     gene            complement(9633..11702)
                     /locus_tag="VFF98_16365"
     CDS             complement(9633..11702)
                     /locus_tag="VFF98_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZU65263.1"
                     /translation="MARKTSRIATDVGGTFTDLVYFETDPDTGAQTIRTAKSDTTPPN
                     FANGVLNVVARAGVALEEVDFFAHGTTVVINALTERKGARTALITTTGFRDVLEIARG
                     NRPDFFNLRYEKPECFVPRYLRREVPGRIDYKGAESQPLDLAGLPDILADFRADGVEA
                     IAVCLIHAYANPAHEQAVIARIAQLWPEVATVASHQITREWREYERSSTAVLSAYVQP
                     VAEKYLSRLDEGLHEKGYGGSLYIMQSNCGVDSLQSAKATPITMVESGPASGFWGAAE
                     LGRLIGEPNVLALDIGGTTAKCSLIEKGEVRIKTDYWIERSNKTAGYPIMVPVVDLVE
                     IGNGGGSIAWVDGFGKLHVGPQSAGSTPGPAAYGRGGTGATTTDANLALGRINADYFC
                     GGTVVADMPAVARALGALAAELGMSEAEVARGILRIANANMVNALKLVSLNRGYDPRD
                     FTLVAFGGGGAMHAAALGQELGVKKVVVPRGAPVFSAWGMMMSDLRRDYFITRLIDGA
                     DSAALSALIGEVCAHAIARFAAEGVGEDRLRLAPLVKLRYRNQEHAVEVPVAAGVVDA
                     AGMAAMIDAFHQTYEREYTYRLDAAVEIVGVHLIASAAVGKLEMSRLPTTGASLEAAI
                     KGQREVDYATDGIHLATIYDAERLEPGMAFSGPAVIEDPGTTIVVHPGNRVSIDAFGN
                     THIAFGG"
     gene            complement(11796..12530)
                     /locus_tag="VFF98_16370"
     CDS             complement(11796..12530)
                     /locus_tag="VFF98_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65264.1"
                     /translation="MSGKRIMITGASSGLGAHFAQLLAADGHQVVLAARRVEALARLA
                     AGIRAAGGAAEVAALDVADPASVAAAFAGLSGPLDVLVNNAGIAAEGPALEMAADAFS
                     AVLDTNLKGVFLCAQAAARAMAGRGGAIVNIGSVLGLRVAGGVAAYAAAKAGVVQLTK
                     ALALEWARHGIRVNALCPGYIATPLNAAFFASPAGQRLIARIPMRRLGALGDLDAPLR
                     LLCSDGAAYMTGSIIAVDGGHLVSGL"
     gene            complement(12617..14284)
                     /locus_tag="VFF98_16375"
     CDS             complement(12617..14284)
                     /locus_tag="VFF98_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZU65265.1"
                     /translation="MTGKPIAPLSEAPLFEAGAGAGEEWWRGAVIYQIYPRSFVDSNG
                     DGIGDLAGIAARLDHAARLGVDAVWISPFFRSPMADFGYDVADYCDVDPIFGTLADFD
                     ALVARAHELGLKVMIDMVFAHTSDQHPWFTASRHDATGAQADWYVWANPRADGTPPNN
                     WQSVFGGPAWTWDARRRQYYMHQFLKEQPQLNLHNPAVQEALLDALRFWLERGVDGLR
                     LDALNHSMFDPDLRDNPVAVDDGRLRTRSYDFQEHSRSMSHPGVVGFVEKIRALCDGY
                     RAIFTMAEVGGDRAEEEMKAYTAGDHRLNSAYGFNFLYARQLDAAVVAEALTSWPGAA
                     RGGHGPAEGWPSWAFENHDSSRASWRWLAGDADEGAVAAFLRVKMALLLALRGNVILF
                     QGEELGLTQDEIPFALLKDPEAIANWPLTLTRDGVRTPMPWDAALPQAGFTTGAPWLP
                     LSPANVARAVAGQEADAGSLLHHTRALLALRKSFPALAQGGLRLLRAEGALLVFERAL
                     AGEVLCCAFNLGGPPLAPPLPEGEILHAAGGAGRAGLPAWSCLIIRV"
     gene            14501..16174
                     /locus_tag="VFF98_16380"
     CDS             14501..16174
                     /locus_tag="VFF98_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZU65266.1"
                     /translation="MATKVVTKAAARIRRFSAACIIACVGLMVVISIVVAAALAHRQA
                     IEARQAYAQQLARQALASSERTADELAQARPLIAATSARTGARKACSDADIETMRGID
                     LASPLLQAVGRIEGNTILCSSIAGQRRIDLGPPDLVSRAGARVWLNVRPMGGQRSYLA
                     IGSGALVGIIHKDLALSMVELMPGAAVSSFNWSHKVMLAARGPFAPAWLRADLAGGSS
                     WRVHGFVLAVARSQRYDLGVVAAAPLNAVPRLPLHTAALLLPIDILAGLLLTMAITRI
                     AEAKASLPAMIAGAMKRGEFFITYQPIVALESGRTVGAEALIRWRRPNGEVIAPDLFI
                     PVAEQAGSIRHLTAHVLDLVARDIGAHIPTAELSHLSVNFSADDMQSPDMVENILAFS
                     RQAGIAMANIVIEATERSLIDIELSSQAIRRLRAMGVRIAIDDFGTGYSSLGYLAQLE
                     IDMLKIDRLFVQALGTGSAASQVAERVIEMARDLKLEIIAEGVETRGQIERLKTLQVD
                     LAQGYYFAFPMPIGELQKRLRTEAGLAPVEMAPVEMAPDTAPRLHAGAR"
     gene            complement(16154..16948)
                     /locus_tag="VFF98_16385"
     CDS             complement(16154..16948)
                     /locus_tag="VFF98_16385"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU65267.1"
                     /translation="MPDEEQRRLLGAFVRARRESIAPDQPAGRRRTPGLRREELAARA
                     GIGSTWVAWIEQGRAVRASAETLARLADGLCLTAAERAYLFTLAGRGDPADPFAAARH
                     AAPPAIAAAVAALAWPAYGLDRAWTVCAANAAARRLFVGLFEGEEAPNLLRYIFTHPA
                     ARALLPDWPGRSARVLAEFRRDYGRAPGDPQVRGVVAWLRLHSEAFRAGWERQSVIAR
                     EGGERSFCHPVDGPLAFTQHTLAEVERGDFRLVFLQPGSASAGAGV"
     gene            17054..17812
                     /locus_tag="VFF98_16390"
     CDS             17054..17812
                     /locus_tag="VFF98_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZU65268.1"
                     /translation="MTRTHETLVDQQFGARAAAYVASAVHASGADLARMAAIAAAHAP
                     AHALDLGCGGGHVSYAIAPYARAVAACDLAPQMLAAVAAEAQARGAHHIATHAAAAEA
                     LPFADGAFDFLACRFSAHHWADVRQGLAEARRVLRPGAPAVFVDVIAPEAPAADTHLQ
                     AVELLRDPSHVRDYRRSEWRAMVAEAGFAITAETPARLRMDFASWTARMATPAPHIAA
                     IRSLQGAASHDVAGHFAIEADGSFTIDTLLIELR"
     gene            17924..18697
                     /locus_tag="VFF98_16395"
     CDS             17924..18697
                     /locus_tag="VFF98_16395"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU65269.1"
                     /translation="MPRFTDKTVIITGAASGLGLAAAHRLAAEGARLLLVDRNAAALE
                     AAIATLPAGSEAAAHIADVARQEEVEAYVARAMELWGRIDGFFNNAGIEGRQNLTENF
                     GAEEFHRVVSINLDGVFFGMMAVLKVMRAQGHGAIVNTASVGGIRGVGNQSGYAASKH
                     GVVGLTRNSAVEYGQFGVQINAIAPGAIMTAMVEGSLRQIGGDDWEEVGRQFVSANPM
                     KRFGRVEEVAALVAFLLSGEALFINGEVIAIDGGQSGKY"
     gene            complement(18749..19552)
                     /locus_tag="VFF98_16400"
     CDS             complement(18749..19552)
                     /locus_tag="VFF98_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HZU65270.1"
                     /translation="MSELKGLFYPRTPSGQASIVPPVPWHYSGELITLEYRTDPAKVA
                     ALLPAGFTLAEEDPGAVAAIWADWQSTSDDPAQILDPIRVQYKEMFIVVRCQFQGKTY
                     SRCVYIWVDKEFAMLRGQHQGYPKKLGSIQMSRPVYVGKAGPRLQPGGTFGVTLAAFD
                     RRLAEGRFTITGAAEKPGFVNGHPMAHNRWMPTIETDGTQSLDEVVTMAGYDVDFGRC
                     FTGDFDLAYFPSPVEELLDLAPVEKIAGYWHEVGVSWKAGTTLYRNNLA"
     gene            19701..20588
                     /locus_tag="VFF98_16405"
     CDS             19701..20588
                     /locus_tag="VFF98_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65271.1"
                     /translation="MTTEYRRILLDGYPILTTREGDRLVTKDGRSIAIDEAIHLPPVN
                     PQKVICVHLNYHSRVNEFMTRLPAAPTYFHKPISALNSHKGHVVRPERCQFLNYEGEI
                     AIVIGKTCRNVSPEEAGDYILGYAVANDYGLHDFRDTDSGSMLRVKGSDTLCPIGPGL
                     VTGWDFRNKAIRTYVNGELKQDGNTADMEWDMHYLVADIARTITLEPGDVLLSGTPAG
                     SRPVQPGDLVEVEVEGLGRLANTIVHGPTPIRDDCGAQPTESEEVISTAMGGDWEFRG
                     QRAARGQGAAFYESTVEEK"
     gene            20595..22028
                     /locus_tag="VFF98_16410"
     CDS             20595..22028
                     /locus_tag="VFF98_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU65272.1"
                     /translation="MGFDMTTGAARFYIGGRWVAPASGASAPIVNPATGAEIGRVALG
                     GRADAEAAIAAARAAFPGWSATSREERLAFLEKIHAGLIARSDEIGAAISAEMGAPGW
                     LGRGPQAGSGPQHFAEVIRVLRDYPFEQPLGGTLIRREPIGVCTLITPWNWPMNQIAT
                     KVAPALAAGCTMVLKPSELAPLDAAILAEIIDAAGLPAGVFNLVHGTGADLGEVLTAH
                     GDVDMVSFTGSTRAGIAIGQQAAPTIKRVALELGGKSANIILAGAPLDQAIPAAVRGC
                     MLNSGQSCNAPTRLLVPRADLDAAVALARTAAEALTIGAPGAEANLGPVANAAQHERV
                     RAHITRAIAEGQTLVTGGPEAPAGLEQGYFIAPTIFIATPDAAIAREEVFGPVLAILA
                     YDTLEEAIAIANDSPYGLSGYVWGATPAEARDVARALRTGMVHVNGAGLDTAAPFGGY
                     KMSGNGREWGVYGLEEFLEVKAIYGGA"
     gene            22069..23073
                     /locus_tag="VFF98_16415"
     CDS             22069..23073
                     /locus_tag="VFF98_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZU65273.1"
                     /translation="MAAPTISAQFLRHAANCVELAGHAPDPLLARLGLDRRQLEEAEH
                     DIPLSAFLAFFEQAAQAVGNPHFGLYAGRMVGADSLGILSFLFLSAPTLREAFASFTR
                     YLETMQQASRNDFVEGRRWGRFQYGIIDDTLAERRQDAEYSIGATYTLARQYTGGALS
                     LEEVRFEHPLVGDYRRYEAYFGCEVHFGQDRNSFSFARSFLDFPGKQLSATLFPVLVE
                     HLSRRRAAEATPDSRAAQVRALIAAAPLDRPPDAPALARALGISVATLGRQLRAEDCS
                     VRDLVAARRMQAARRLLRDGQRSIADVAIATGYAESASFIRAFRRHFGAAPGHVRRQG
                     "
     gene            complement(23165..25402)
                     /locus_tag="VFF98_16420"
     CDS             complement(23165..25402)
                     /locus_tag="VFF98_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65274.1"
                     /translation="MSKIGKSVRCVSVLALAAALPQMARADEAAPAPAATAPGEIVVT
                     AQKREQNVQKVGIAITALGKDTIAAIGRQDVTALAGQVPNLEVNQYAPTITVFNIRGV
                     SQNDFTDAQEAPIAFYDDEVYVASLGAISGMNFDLERVEVLRGPQGTLFGRNATGGLV
                     QFVSAKPTARPEGYLTLTTGSYGQVATEGAVSGAVTDALRARFSFTTNNAGGYIRNTM
                     GPSLGNAKFYALRGQISADLSPRDKLNIKVQTLRNDHERSGGLYSWGASYADPNNHGL
                     GTFTSTDANGYSNTSGNPFIQSEDRVGSFNRTYWDVSARYSHDFGAFDLTSISDYQHL
                     HKDYGEDSDMSPNNAFNYDTHQHMYQMSEELRLSGKTERLNWVGGVYLLDIHTANHYQ
                     INSPVTAIPGIGLLDYGGTQNTFSLATFAQLEYAITPKVSVIGGLRYSHDRKHIEFAD
                     YANGELMFDWATSIPSVAALRFRTFDNWSGKAEIDYKPNNATLLYASVNRGTKSGGFG
                     TVSSNTPYTDSAAVSSSLGYPLVQAIPFGQEVLTNWEGGFKLTMLNRTTHFNGSVFFY
                     NYQGYQAFQNVGVNQYISNHQAYEHGAEFEFNTRPVRGLYLGTFLTLLSTKVKNITLP
                     DGTVADRVMPQAPKTSIGGQARYEFAAGPGKLAISTDWKYDSGEYFETNNAPVDFEPG
                     HTVGNVRVSYALADGKYELAGFVNNVTDKWYRVYNLDLSSLLGASNQTYARPRTWGVS
                     ITAKY"
     gene            complement(25568..26743)
                     /locus_tag="VFF98_16425"
     CDS             complement(25568..26743)
                     /locus_tag="VFF98_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65275.1"
                     /translation="MSARQEAGFHEVDVNGWPAVASAIVLGTIGVLSFIIQPGLVEGF
                     VRHLAVSEAQAVNLVGLEMLGVALATVGLALLGGRIGWRWVVAAGLLMAAAGDAASAL
                     STGSAGFAPARVLAGLGEGVIISISFTFVGITLRAERNLALYLSLLLTYGAFGLWFLP
                     RFLDAYGLPVLFAGFAGLSLAALLLLPLLPHRFSAADMANPEARQLPLALLVVALAGV
                     LSYNLAQGIAWAILALVGAGAGLADQQIANALFLSQILAIFGALTSVFLAARINRDHA
                     IAFGILVGAASIALLVGHPGVGLFTLAVCGFNVLWNFVLPFILGRICDFDESGRIMGF
                     AIAMQMTGLGGGPMLAALLLDGHGYLPVELLCVGLFLASYALLRLPIRRHRGLLARG"
     gene            26993..27982
                     /locus_tag="VFF98_16430"
     CDS             26993..27982
                     /locus_tag="VFF98_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZU65276.1"
                     /translation="MAALDGWAGVLANKGINASDVMSPAPDQAPCATDSAMMPLKDFV
                     RFSEAVVAHSRDAALPWLAGTHYDLSSLGSVGDAINTAGKVGSAIRRFVEYFSLLQDC
                     TDIHFGLEGPMACVSYRILDPDIWPRHYDAMFSLGIIGQLIRRGRHGGWDKVEFAFEA
                     EQGELRSNIAQVVQAPCTFGADTNQIRFPAAFLDLPLPVARRADDMRPLGRALVAKRR
                     ATPVSERLAQLVFRDLNALAIDQDRIAREIGMSTRTMRRKLAEEGSSYQQVLDECRMR
                     QALFEFRTRPDLSIAQIALRLGYAEHSNFTRAFHRWSGQSPQAYRASLAHPQH"
     gene            28015..28857
                     /locus_tag="VFF98_16435"
     CDS             28015..28857
                     /locus_tag="VFF98_16435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZU65277.1"
                     /translation="MTRLLLLEGNTADKRALGRQLGVRSSSEIYAEAIAAHYPAFELD
                     VLNGADADGAIPHGRSWSDYDGFVITGSSLHAYDTDFAVTNQIELTRQAARAGLPVFG
                     SCWGLQIAVMAAGGLVARNPAGREVGFARKIWRTPAGASHPIFAGKAPVFDAPCIHYD
                     EVVRLPEAATLLASNAHSLVQAAVIPLEASEVWAVQYHPEFDLLQLVQLYTLYAEDMI
                     AQGFFADEAELVAYRGKIAALAANPDKAGLAWQLGVDADVLDDTQRRGEILAWIEAKV
                     LGNR"
     gene            complement(28904..29773)
                     /locus_tag="VFF98_16440"
     CDS             complement(28904..29773)
                     /locus_tag="VFF98_16440"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HZU65278.1"
                     /translation="MGEIIGAGIVAHAPTIMMSEQDRYELNEGKEISLVPGLRRLRSE
                     VLDALKPDTIVIFDTHWFTIVEFVVSAHARRSGLYTSDELPRGMKQIPYDLKGNPQLA
                     TEMAAAATRHGIRTTPIDDPWLPIHYPTVNLSTFLDRGEEWIGVSHAYSADPEDFLKV
                     GAGIGEAIRASDKRVVLIASGSMSHRFWPLRLIPAHEASDPIHISTPEAREADLERIA
                     WFQEGNHAAVIDSMPQYLRHAPEARFGHYLMMAAALGGREWTAKGRLFSDYENATGTS
                     QVHVWFDRPEGGW"
     gene            29923..31188
                     /locus_tag="VFF98_16445"
     CDS             29923..31188
                     /locus_tag="VFF98_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU65279.1"
                     /translation="MRFAFSRSNDAHVDLSSLDAFNEGAPFATFDRLRREDPMAWSDM
                     ANGDRGFWSVTRHADLLELNRQADLLSSARGIRMEDQSQEEYEARKTFQETDAPMHRA
                     FRALVAKAFAKHTVAQYEEQIRAIVTDLLDVALEEGTFDAVDRIARRLPMQMLAQIMG
                     VPTEDGPWLVEKGDALISNADPDYTDFVVDKVDTEAYRLLPFRSPAAVELFDYANSLL
                     ARMDKGEQIGVLNLVLQETSHGTSMSRDEFRNFFCLLVAAGNDTTRYSISATIHALAN
                     NPHLLAQLKAGQFLGWEEAADEMIRYASPTSHFRRTATRDFTFHGKDVKAGDKVLLWF
                     LSGNRDETAIEDPYAIRLDRPRNPFLSFGQGGPHICLGMWLAKLEVAIVLQELAKRIE
                     HIEQAAEHSYLRSNFIHGIKHLPVRVRAA"
     gene            31218..32540
                     /locus_tag="VFF98_16450"
     CDS             31218..32540
                     /locus_tag="VFF98_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZU65280.1"
                     /translation="MTIQHPFDAHAAAFAPIERCRLLFADQLNLARGKYVPMSEVGKG
                     HARLCVGTYAVTYDKQMVAAPGGGVLDGLPDIELRFDPTALRPGWEPGTRVALGDLAY
                     KGAPFALCGRSALKQAIAAWRERGLEPMVGIEMEAYVFQRGADGSWVPYDTPGAFVYG
                     TGPFTDPAGLVDDIWQAAQHCGLPIESINAEFDAPQFELTLRYADALSAADDSFLFRQ
                     MAREILYKRGYLLSFLPRPIAGKSGSGLHFNLSFTDPQGRNVFAQDVVNGPGEVMSGA
                     IAGLLHHHEALAGILAPLTNSYERLQPASLSGYWANWGIDHRSTTVRVSAETGQAARI
                     EHRMADGAASPYFALAAILRAALLGLEHGYALPPVETRDGLEETGTSRHTPHDLASAL
                     DALAADTELTSAIGAELVDNYLTIKRTEVADLAGKSFDEQVAYYLHYI"
     gene            32549..34012
                     /locus_tag="VFF98_16455"
     CDS             32549..34012
                     /locus_tag="VFF98_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZU65281.1"
                     /translation="MSSPRIDIEGIAVSPDHFIGGERVASPTTFETRCPFDWERKLAD
                     IARGDADTAALAAAAATQAFPAWAALSPAERGAYLHRLADLIEANVEKLALIECLDMG
                     MLHESLRLRVILRGAANFRNYADLASAHEERVWSSRGTLNRVIRMPAGPSLIITPWNA
                     PFMLSTWKCAPALAAGNTVILKPADWSPLSASVLADLIAEAGFPAGVFNIVQGLGAEL
                     GNALTSDPRIKRISFTGSVPTARVIGRAAADNIVPFTAELGGKSPLLVFADADIDAAA
                     KKAAGQYDDSGQVCMAGTRIIVEDSVKEAFLERFHHYADQHVMGSSLDPATTMSALIH
                     PVHAERVLGFIDRARADGDTIARGGRRWKEGQNWIEPTLILPKSNQSECVQSEIFGPV
                     LTFQTFVGEEEGVALANSTAYGLSCICYTGSEERAGRVGRQLRGGTVWVNTFLVRDLT
                     APFGGIGISGIGREGGDYALDFHSDLKTLQILEGSVA"
     gene            34184..35644
                     /locus_tag="VFF98_16460"
     CDS             34184..35644
                     /locus_tag="VFF98_16460"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZU65282.1"
                     /translation="MSATITAPAALTPAPAPAAAPAHAGGFLASVDAMYARAAQLVPM
                     EEGLSEKIRICNETYITRFGVRLRGRMYTFEGWRCVHTNHPAPAKGGIRYAPDVDQDE
                     VEALAALMSYKCALMGLPFGGSKGALKIDPAQWTPSELERITRRFAQELIRQNFLSSS
                     LNVPAPDVGTSEREMVWIADEYRRMRPHDINADACVTGKPLAAGGIEGRPEATGRGVQ
                     YAIRALFNTPADVARAGLPADLCGRQVIVQGFGNVGYHAARFLAEDGCLITGIAERNG
                     GIVNPQGLDIAALHTHWRTHGTFLGFAGGDFVPRGEDLLEMACDILIPAARESVITGA
                     NAARIQAPLIVEAANGPVSHEADARLRAAGKVIIPDLFANAGGVTVSYFEWVKNIAHL
                     PFGLMERRQRQRDTSQLTSALEQLLGVAMPDELAQIGAAREIDLVRSGLEEKMRATYA
                     QMSHLWNSAEQIDDLRTAAYVIALTRIRGLYEAIGI"
     gene            35745..36239
                     /locus_tag="VFF98_16465"
     CDS             35745..36239
                     /locus_tag="VFF98_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65283.1"
                     /translation="MNGATGESARLEANRQLVRAYLDAVSFWKLDVITALSTEDVVFE
                     VPFCPPGFARVTQGRTRYVEVLRQASTLMIDGPENLHAITVDCLAGQPDELVAAYKST
                     MVMRSGAAYANEYLARFSLRDGQVARFVEYCDPIRLYVAMGGQVPPLQALADSSMLPD
                     LLRG"
     gene            36348..38114
                     /locus_tag="VFF98_16470"
     CDS             36348..38114
                     /locus_tag="VFF98_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZU65284.1"
                     /translation="MALFGNLRIKTISLAGTTVLAGVAIVCAACVLVVSRQIGDNLAY
                     AENNTIPSIVALNGIGTGLTAVRLDLGHAVAARDGATRQAALAKYAADTATVDSRVKA
                     YTPFISDDAERRVFDNLTTRWGQYHDLAEQLAQMANRDPEAAYRLWEGPLGQLGPQLF
                     TALEADVAYNQGIGARAAAAGNAAMARANLLAWVLIIVATAVGAGIVSLLLLRVIGPL
                     GRLTGAMNEMAAGHLDRQVPGLEQHDEIGDIGRALLAIKQSVAARAEAEAHAREAVQQ
                     QVVSALGNGLAALKAGQLAISIDARFPEEYVQLRGDFNAAVATMADLMRQVAGSAGSV
                     HSGASEISAAAADLANRTQGQAANLEESAAAVRELTQSVTSAAATASEAATLARTAQG
                     NAATSGEVMARAVQAMDQIAQSSRRMEEIVALIEGIAFQTNLLALNAGVEAARAGDAG
                     KGFAVVATEVRALAQRSSDAAKDITGIIRSSGHDVATGVEMISQTQSTLGQIVSGTEE
                     LAIMIDHLAEAAREQSAAIMQVDSVVGEMDRATQQNAALVEESTAAARSLASEANVLS
                     GLVSRFDLGQAAGGHTSRRRAA"
     gene            complement(38120..>38423)
                     /locus_tag="VFF98_16475"
     CDS             complement(38120..>38423)
                     /locus_tag="VFF98_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZU65285.1"
                     /translation="ADVTPSLRQAIAAYFASAGAEPLIRLETQLQQTIVSLVAEGIGL
                     ALVPASLERLGMRGVVFRPLVAAPVVEQVLVWRQANANPALPHFLAAARALAEGAG"
BASE COUNT         6399 a        13384 c        12594 g         6046 t
ORIGIN      
        1 ccctgtcgca gcgccgtgac ggcgatctgc gagccgccgc tgcccaccag atgcacgctg
       61 gccggcctga tgccatagct tttgagcgcc atttccaccc cgatcgtctg gatatcggcg
      121 gtggagacga cggcatcgat gcggcggttc acctgcagca catcgagcat gccgctcatc
      181 gccgtgccgg catcgtaatt gccctcgatg gtggccacca cgcggatgtt gcgatggggg
      241 gcgaaggtcg ccagataggt gcgatagcgc agcaggtcga agggatagac cttctgcccc
      301 accaccagca ccacgttgca ctgcggccgg ccggcgcaat aggccaccac atcctgcgcc
      361 tgcgcgcgcg cgccatcgct gggccgggcg gccacggtgg ccaccacgcc cggcacttgc
      421 ggggccagct catccagctg cgggccgatg ggaaattcca ccgccaccgt gcggatgccc
      481 accgcgccgg catggcgcac ggccgaggcg atcgacacgc tgtcgttggg gaagaggatg
      541 atgccatcgt agcggtgcga ggcgatcaca tcctccagct ggttatactg gagcgtggaa
      601 tcgaaagcgc catcatagag cgtgacctcc accccccgct ggcgcgccgc tgcctgccgg
      661 atgccctgcc agacagactg gttgaagcca ttttgcgcgc ccgccgccag aaaggcgatg
      721 cgcaaggggg ccgggcgcaa gggggccggg cctttgggcg ccggggccgc cccgcccagc
      781 gccagcgcgc ccgccaccag caggccagcc cgacccatac tggcccatac ccgccgccgc
      841 cctgccgcag ggggcagcgc gccggaacct tgcgcggcat tttcaacagg atcgatcaag
      901 atggcgcggc tccgattgtg cgttgaagag atatggtata cggtattttt tcttgcgcaa
      961 ggagatttgc atgcatcccg acatggacag ccccaccctc gccacgttga ccgggctgat
     1021 cgccgcgccc ggccagcctg atgccctgct gacacggatg gcccgcatgc tgatggcaga
     1081 cccgggctat gccatgctca ccatcctgct gtatgatgtg gccggcgggc gcgggcgccg
     1141 catctattcc accgatgagg tggcctatcc caccggcgcc ttcaagccca tcccgatgac
     1201 cgactgggcc gaccaggtgc tggtgcgcca ccagccgttc atcgccgata ccgtggccga
     1261 tttccgcccg cattatggcg attgggccaa gctggaggcg atggggctga tcagcgggct
     1321 caacacgccg atcatcgtcg atggcgtggc gatcggctct gtcaacttca ccgcccgctc
     1381 ggaaggctat tacactcccg cgcgggtgga ggccgcccgg cgctgcggcg cgctggcggc
     1441 catcgccctg ctgctggaag cgcgcggcgc ggcctgagcc ccacgcccag gccgcgcggc
     1501 ctgggccgct tcagatcgga tcatcgatcg aggcggcggg gcgggtgaac cattccgccc
     1561 cgttctcggt cacatggaaa tggtcttcca gccgcacgcc gaagcgatcg gggatgacga
     1621 tcatcggctc gttggaaaag cacatgcccg gggccagcgg ggtcttgtcg ccgcgcacca
     1681 gataggccgg ctcgtggatc gacaggccaa tgccatggcc ggtgcgatgg ggcaggcccg
     1741 gcaggcgata atccggcccc agcccggccc cttccagcac cttgcgggcg gccgcatcga
     1801 tctcctcggc cagcacgccc gggcgcaccg catcgaaggc ggcctgctgg gcggcgcgtt
     1861 ccagcgccca gatctcgcgc tggaaggcat cggcctcgcc aaacacatag gtgcgggtga
     1921 tatccgagct gtagccctgc accgggcagc cggtgtcgat cagcacgata tcgccctcgg
     1981 ccagcacggc atcgcccggc aggccatggg gaaaggcgct cgaccggccg aattcgacga
     2041 tgcagaagga attgcccggc gcccccagcg cccggtgggc cgcctcaatg aagcggcgca
     2101 cctcgctggc ggtgatgccg gcgcgcatga tccgggcggc gcggcggtgc acttccagcg
     2161 tcatctgctt ggcctggcgc atgatcgcca gttccgcggc agatttgatc atccggcagg
     2221 cgttgatcgg gccggccaga tcgaccacct ccacccccgg tgccaccgcc cgcagccggc
     2281 ccagcatgcc aaagggaaat tccggatcga tgcccagccg ggccacttgc ctttcacgca
     2341 gcacatcggc caccagcgcg aaggggttct cctcttcctc ccagctgcgc acgctggccg
     2401 caatcgccag atcggccgcc agcgagccca gctcgaaagt ggggcagatc agcaccgggt
     2461 ccccctccag cggcaccagc atcgccacca gccgctcggt ctgcccccag ggcacgccgg
     2521 cgaaatagcg caggctggcg ccggcatcca ccagcgccat gtcgatgccg gccgcggcca
     2581 tgtgcaggcg cagcttgtcc agccgggcgg cgcgttccgc cggggtgatg agcggcgcca
     2641 catccgccca gggcttgaga tcggcaagct cgcttgccgc attcgatccg ccgattgcct
     2701 cagtcataac cattcctcat ttatgtggaa gcgccgcgcc gggcgccgaa acgttcgata
     2761 tcgaaaggcg ccagcgacac cggcggcgcg cgcccctcca ccagatcggc gatgatctcg
     2821 ccgctgagcg gcccgagcgt gagacccaga tgctggtggc caaaggcata gagcaggttg
     2881 gtgtggtggc ggctgcggcc gatcgccggc agataatcgg gcaaggtggg gcgcgcgccc
     2941 agccattccg ccccgggcag ttcgaaaggc aggcccagtt cgctgatgcg ccggcgcagg
     3001 gccgcccatt tggccgggtc tgccggcgcc cctggccgcg ccagttcgac aaagctggaa
     3061 gcgcgcacgc tgtcctcaaa gcgggtgacg atcatcgccc ggtcctcgaa caccaccggc
     3121 ggcagatcgg ccggccagcg cgcgcccggc gcctgcaggt gataaccgcg ctcggcgatc
     3181 atcggcacgg caaagcccag cccctccagc agcggccgcg acagcacgcc gccggcaacg
     3241 ataatctcgt cggccagcag gggcgtgcca tcggccagtg cggccagcac cgtggcgccc
     3301 tctgcccaca gccgggcccg gccctgcagc cagcgcccgc ccagcgccgc aaaacgcgcc
     3361 cgcagcgccg cttccagcag atcgtggccg gcaatgctgc cgctgccctc gcaatagagc
     3421 gcgccggcgg gcgccttgcc accccggagc gcggcgatct gcgcctcctc gtcggccgtc
     3481 agatcgcgaa agcggatgcc ctcgggcaca tcggcctgcc aggcctggcg gccgcgcgcg
     3541 gcgctctcgg gccggtccca cacgatgata tggccggtgc ggcgcagcag agcgggcgcc
     3601 cccagcgcct ccacatagcg ctgccaggcc ggcatcgccc gggccagcag gccagacagc
     3661 gccgcctgcc cccgcgcaaa ggccgatggc cgcgccgccc aggccagccg ggcgacaaac
     3721 ggcaaccagc ggcccgcctc gcgccagggc agcgccagcg cgccatcgcg cgccagcaac
     3781 tggcgcggca cgcccatcac cgtggcgagg gaggccagcg gctccacctg ctcgatggcg
     3841 atatggccgg cattgcccca ggaggcccgc cggcgcggcc cctcctcatc caccagcgtg
     3901 acagcatggc cccgccccag cagcgccagc gccgtggccg agccgacaat gccgcccccg
     3961 atgacgagga tgcggcggct ttcggatttc acacttccca tagtcactct tatacggtat
     4021 acgaagttcg aagcaacgga gtttcgcaat ggcagaattg aattggaagg gtgtctaccc
     4081 ggcggcaacc acgcagttcg ccccggacct ttcggtggat gtggcagcca gccagcaggt
     4141 gctggacggg ctgatcaggg atggtgtcaa cgggctgatc ctgctgggca cctgcggaga
     4201 gaacaactcg ctggaagcgg atgaaaagca caccctgctg gaagccgcgc gcgaagtggt
     4261 ggccggccgc gtgcccaccg tggcgggcgt ttcggaactg accaccgcgc gcgccgtggc
     4321 ctttgccaaa accgccgaac aggccggcgt cgacgggctg atgctgctgc cggcgatggt
     4381 ttatgtgccc accgaggagg aactggtcgc ccatttcaag gcggtggccg cggccaccag
     4441 cctgccgatc atgctctaca acaacccgcc ggcctatcgc acctgcgtga ccgagggcgc
     4501 gctgcgccag ttgctggaag tggagaatat cgtcgcggtg aaggaaagcg cgcccgatcc
     4561 gcgccgcttc accgatcttc acaatgcctt tggcgagcgc ttcgtgctga tggcgggcct
     4621 tgatgatgtc gcgcttgaag ggctgatgct gggggccagt ggctgggtct cgggcctcac
     4681 cagcgctttc ccgcaggaat cggtggcgct ggtggccgcc ttcgagcgcg gcgactacga
     4741 caccgcccgg cgcatctatc gctggttcat gccgctgctc catctcgatg cggagcatga
     4801 tctcgtccag tcgatcaagc tggccgagca gatcatgggc cgcggctgcg agcgcgtgcg
     4861 cctgccccgc ctgccgctgg ccggcgcccg ccgtgcccag gtgatcgcca tggtggaaaa
     4921 ggccgccgcc acccgccccg gccttgcccc gctggatgcg gtggcctgag atgcgccaca
     4981 cgttcttctg catcgacggc cacaccgccg gcaatcccgt tcgtctggtg gccggcggcg
     5041 cgccgcaggt gcaggggaac tcgatggcag agcgccgcgc cgatttcctg gcgcgcttcg
     5101 actggatccg cacgggcctg tgtttcgagc cgcgcggcca cgacatgatg tcgggcgggt
     5161 ttctctaccc gcccacgcgc cccgataccg atatcggcat cctcttcatc gaaaccagcg
     5221 gctgcctgcc gatgtgcggc catggcacca tcggcatgat cacctttggc ctggaacacg
     5281 ggctgatcgt gccgcgcacg cccggccgac tgaaagtgga agtgcccgcc ggcctcatcg
     5341 acatcgacta tggcgaggag gccggcaagg tcactttcgt gcgcatccgc aatgtgcctt
     5401 cctatctggc gcgggcgggc atcgccatcg aggtgcccgg ctttggcccg ttgctggtgg
     5461 atgttgccta tggcggcaat ttctatgcga tcatcgagcc gcagggcccc tatcgcgggc
     5521 ttgatgatct gggcgcggcc cgcctcatag agctcagccg cagcgtgcgc accctggtgc
     5581 aggcggtgta tgagccggtg catccgcagg atgcgcgcat tcgcggggtc agccatgtgc
     5641 aatgggccga tcgcccctcg gcgcccgatg ccgatggccg caatgcggtg ttctatggcg
     5701 aaaaggcgat cgacagatcc ccctgcggca ccggcacatc ggcgcgcatg gcccatctct
     5761 ttgccactgg ccgccttcag gtgggcgatc gtttcattca ccagagctat atcggcagcc
     5821 gcttcaccgg ccgggtggaa gccaccgccg atgtgggcgg cctgcccggc atcatcccct
     5881 cgatcgaggg ggcggccgtg gcaaccgggc acaacaccat ctggatagac cgggaagatc
     5941 ccttctgggc cggtttcaca gttgtgtaga gccgccccca cgcggtttac ttatggtgtc
     6001 gccatggtag gacacaaaac atgagcatcg tcatccgatc cttgtccgat caggtttttg
     6061 acgttctgcg cgagaggata ctggccggcc aagtgcccac gagcagtccc attcgccagg
     6121 acgcgctggc ggccgaactg ggcatcagca agatccccct gcgcgaggcg ctggcccggc
     6181 tggaacagga aggcctgctg gaatcgcaga ccaaccgcgg cttcttcgtg cggccgatga
     6241 gcacgcgcga gctggaagag gtctatgccc tgcggctgaa gctggagcct gacgcggtgg
     6301 tggccggcgc gcgggccgcc acgcccgccc agcaggaagg cgcccgcaag gcgctcgacg
     6361 atttcaagcg cgaggccgat ctgcggcgga tttccggcgg cgcccacaac cgcgccttcc
     6421 atgtcgcgct gatcgagccg agcggcaatc gcgtcaccaa tgatttcctc gtgcgcttgc
     6481 atatcctggc cgatcgctat gtgtgcaagc atctggaacc gctgggccgc aacgagcggg
     6541 ccgacaagga gcatgacgag ctgctgggcg cctggctgga ccaggacgag ccgcgcctgc
     6601 gccagctggt ctacgaccat atcttcaaca cgctggcaga cctgcgcgag cagctcgccg
     6661 ccgaaggggt gagcggctag gccgagagcg ccggccgcac cccgcaccac agcgccacga
     6721 acagcgccag cgtgatcgtc gtctggcgca tggattcgag gtcagcatat tcgttggccg
     6781 cgtgaatgcc cccgccgcta gagccatagc acagcgccgg tatgccataa tgcacgccat
     6841 actggcgcac atcggtggtg gcgggcaggc agaagctttc cagcggcgcg ccaaacacca
     6901 gctcatgcgc gctggccagc gccgcttcgg ccgccccgcc cggcgccagc acgtaagggt
     6961 ctgcggcaaa gccgatccag ctcacctcgg gcacggcgct gccgatccgg gccgcgcatt
     7021 gcgccacctg cgcgcagatc gcctcgcgca ccgccgcgac agactggccc ggcagcacgc
     7081 caatgcggca atccgcctcg caccaggccg gcaccatgcc gatccagtct cccccgcaga
     7141 tcttgcccat cgagaaagtg accggctgcg ccatcgcgcc gaaccacgga tcctccgccg
     7201 ccagccggtt cgccgcggcg gccagatcct gcagcgcgca cgcatggtca tacatctgca
     7261 ggatggcgct ggcgccgggg ccgcgttcca gaatatgcgc cggctcgccc agcaggcgca
     7321 ggcgaaacca cacagagccc agctgcgcgc gcagcagccg gttgcccacc gcctcgggga
     7381 tcaggcaggc ctcggccaca tggccgcgca gcagcgtgcc cagcgcgcca ttgccggtgc
     7441 attcctcttc cgacaccgtc tcgacgaaga tatccgccgt cggctccagc ccgatcgccg
     7501 ccagtgcctc cagcgcgaac accatcgccg ccacgcccac cttcatgtcg gcagtgccgc
     7561 ggccatgaat gcggccatcg gcgatctgcg gatcgaaggg atcgtggcgc cacagctcgc
     7621 gcgggcccgg gggcaccaca tcgatatggc cctggaggat caggctgcgc ccttctgtca
     7681 cgcgcgcggg cacctttgcc accacctgca gcgcctctgc cagatccacc tccaccgccg
     7741 gcgaggcacc ggccgcccct gccagcgcca catcagccag gctgaacaga tccacctgcc
     7801 agccccgccc ggcaaactgg ccggccagcc agcgctggca atcgccctcc tgcccgcgcg
     7861 tgctggcaaa gcgcaccaga tcggccagcc aggcggtctg gcgcggcagg gcggcggcgg
     7921 tggcctcctc cagccgcgcc ttcagccccg ccagatcgtt cacccccgcc atcagccggc
     7981 gtagccgtag atcgcccggg cctcttcggc ggtcagatag ccatcgcgga tatcgcggcg
     8041 caccgcttcg ggatcacgcg cgtgtgggtc gccgcagccg ccgccaacgc ctgtcaccac
     8101 atgcaccaca tcgcccttgc ccaggggaat gttggtgcca aaggcgtggc gttcgggcgc
     8161 cttgccggcg cgcagcacct cgacataatt gggcgagcca tccgcgccgc cattgatgcc
     8221 ccagggcgga atgcgcgagc gggtgtagcc gacgctgagg aagttctcgt cggcccgcac
     8281 gcgatagcgc agcgagagcc ccttgccccc gcgccactgg ccagcgcccg cctgggtgat
     8341 gttgagcgcc atctcctcca cccccagccc atagcgggcc tcggccactt cggccgggca
     8401 gttgaaggtt tcgccatgaa agccggagaa gatggcgttg ttgccatcgc gcccgtcctc
     8461 cgcgccccag ccgcccagct gcggctccac caccgtataa tgctcgcccg tctcggcatg
     8521 tttgccgccg atgttggtgc cgcagatcga ggagaaattg cccgccggca ccagatgcgg
     8581 aaactgcgcg gcaaggcagc gcaggatcag gtcgaacagg cggatctcca cctcggcata
     8641 atagcccagc gcgcccggtt cctgcacatg atagacagag cctggccggg tgagcacctg
     8701 cagcgggcgg aagaagccgg cattgcccgg cgcctcggta tcggtcaccg ccttgaaggc
     8761 cagctgggcg gcgatctcgg tgccctcgcg gctggaattg ctggagccgg cctgatcggg
     8821 attgtcgcgc acatcgatga tgaaggcctc atcggtgatc tcgatggcga tcttgtggat
     8881 ggcgccgttg tcctgctcct cggcgaaggt gaagcggccc ttggggatgc gcgccagcgc
     8941 cgcgcgcgcg cgccgctcgc ccagctccat gaacgaggcc atcgccgcca tgaaagtgtc
     9001 cacgccatat ttcgtggcca tttccagcag ccgccgctcg ccgatgcgga cgccggcaat
     9061 gcccgcccac agatccccgc gcaggaaggc gggcaggcgg gtgttggtct ccagaatgtc
     9121 gaacaccgcc tggttgggca caccgccggc gatcagcttc accgccggca cgcgcaggcc
     9181 ctcctggaag atctcggtcg cctcggtgga catcgagccg ggcaccgagc cgcccacatc
     9241 attgtaatgg gcgatattgg ccgaccaggc gatcaaccgc ccctcggcga acaccggcat
     9301 ggcgatgatc atgtcgttga gatgggtgac gccgccgcaa tagggatcgt tggaggcaaa
     9361 cacatcgccg ggccgcagcg tttcggccgg atgcttctcg acgatgcgca gcaccgcctt
     9421 gtcgagcacg ccgacgaaag cgggaatgcc ggcgcccgaa gcggcgatct ggccctgcgc
     9481 gtcgcagatg ccggtgccaa ggtccagcac ctcgtagatc accgcgctca tcgaggtctt
     9541 gcgctgcacg gcgaacatct cgtcggcggc ggcctgcagg ccgttctgga tgatgtcgaa
     9601 ggtaaaggcg tcgtgggggg aagtggccat gatcagcctc cgaaggcgat gtgggtgttg
     9661 ccgaaagcat cgatgctgac ccggttgccg gggtgcacga cgatggtggt gcccgggtcc
     9721 tcgatcaccg ccgggccgct gaaggccatg cccggctcca gcctttcggc atcgtagatc
     9781 gtcgccaggt ggatgccatc ggtggcataa tccacttcgc gctggccctt gatcgccgct
     9841 tccaggctcg cgccggtggt cggcaggcgg ctcatctcca gcttgcccac cgcggccgag
     9901 gcgatgagat gcacgccgac gatctcgacc gcggcatcga ggcggtaggt gtattcgcgt
     9961 tcataggtct ggtggaaggc atcgatcatc gcggccatgc cggcggcatc cacaacgccg
    10021 gccgccaccg gcacttccac cgcatgttcc tggttgcgat agcgcagctt gaccagcggc
    10081 gcgaggcgca gcctgtcctc gcccacccct tctgcggcaa agcgggcgat ggcatgggcg
    10141 cacacctcgc cgatcagcgc ggaaagggcg gcgctgtctg ccccgtcgat caggcgggtg
    10201 atgaaatagt cgcggcgcag gtcgctcatc atcatgcccc aggccgagaa caccggcgcg
    10261 ccgcgcggca ccaccacctt cttcacgccc agttcctggc ccagcgcggc ggcatgcatg
    10321 gcgcccccgc cgccaaaggc caccagcgtg aaatcgcgcg gatcatagcc ccggttgagc
    10381 gacaccagct tgagcgcatt gaccatattg gcattggcaa tgcgcaggat gccgcgcgcc
    10441 acctcggcct cgctcatgcc cagctcggcc gccagcgccc ccagcgcgcg cgccaccgcc
    10501 ggcatatcgg ccaccaccgt gccgccgcag aaataatcgg cgttgatgcg gcccagcgcc
    10561 agattggcat cggtggtggt agcgcccgtg ccgccgcggc cataggcggc cgggcccggc
    10621 gtcgatccgg ccgattgcgg ccccacatgc agcttgccaa agccatccac ccaggcgatc
    10681 gagccaccgc cattgccgat ctccaccaga tcgaccaccg gcaccatgat cggatagccg
    10741 gccgtcttgt tgctgcgctc gatccagtag tcggtcttga tgcgcacctc gcccttctcg
    10801 atcagcgagc atttggccgt ggtgccgccg atatccagcg ccagcacatt gggctcgccg
    10861 atcagccggc ccagttccgc cgccccccag aagcccgagg ccgggcccga ttccaccatg
    10921 gtgatcggcg tggccttggc cgattgcagc gaatccaccc cgcagttcga ctgcatgata
    10981 taaagcgagc cgccatagcc cttctcgtgc agcccctcgt ccagccggct gagatatttt
    11041 tccgccaccg gctgcacata ggccgacagc accgccgtgc tggaccgctc atattcgcgc
    11101 cactcccgcg tgatctggtg cgaggcgacc gtggccacct cgggccacag ctgggcgatg
    11161 cgggcgatca ccgcctgctc atgcgccggg ttggcatagg cgtggatcag gcagacggcg
    11221 atcgcctcca ccccatcggc gcggaaatcg gccaggatgt cgggcaggcc ggccagatcc
    11281 agcggctggc tctcggcgcc cttgtaatcg atccggccgg gcacttcgcg gcgcagataa
    11341 cgcggcacga aacattccgg cttctcgtag cgcaggttga agaaatccgg ccggttgccg
    11401 cgggcgatct ccagcacatc gcgaaaaccg gtggtggtga tgagcgcggt gcgcgcgccc
    11461 ttgcgctcgg taagcgcatt gatcaccacg gtggtgccat gggcgaagaa atccacctcc
    11521 tccagcgcca ccccggccct ggccaccaca ttgagcacgc cattggcgaa attgggcggc
    11581 gtggtatcgg atttggccgt gcggatcgtt tgggcgccgg tgtcgggatc ggtttcgaaa
    11641 tacaccagat cggtgaatgt gccccccacg tccgttgcga ttctgcttgt cttccgggcc
    11701 atgaatgtcc tcgataaaat gcgcgcggag gacgtttgcg cctccaatcc ggaagcatag
    11761 tatacggtat tttatattac gcaaccccgt cgccgctaca gccccgagac cagatggccg
    11821 ccatccaccg cgatgatgct gccggtcata taggccgcgc catccgaaca cagcaggcgc
    11881 aagggcgcat ccagatcacc cagcgccccc agccgccgca tgggaatgcg cgcgatcagc
    11941 cgctggccgg ccgggctggc gaaaaaggcg gcgttgaggg gcgtggcgat atagcccggg
    12001 cacagggcgt tcacccggat gccatggcgc gcccattcca gcgccagcgc cttggtcagc
    12061 tgcaccacgc cggctttggc ggcggcatag gcggccaccc cgcccgccac cctgaggccc
    12121 agcaccgagc cgatgttgac aatcgccccg ccgcgccctg ccatcgcccg ggcggcggcc
    12181 tgcgcgcaca ggaacacccc cttgagattg gtgtcgagca cggcggaaaa agcatcggcc
    12241 gccatttcca gcgcggggcc ttcagccgcg ataccggcat tattgaccag cacatccagc
    12301 gggcccgaca ggccggcaaa agccgccgcc accgaggccg gatccgccac atccagcgcc
    12361 gccacctcgg ccgcgccgcc tgccgcacgg atcccggcgg caagcctggc cagcgcttct
    12421 acccgccggg cggccagcac cacctgatgc ccgtctgccg ccagcagctg ggcgaaatgg
    12481 gcgcccagcc cgctcgacgc gccggtgatc atgatgcgct tgccactcat cccacgctct
    12541 ccccagcccc ggcaaaaccc cggcataggc ccactgcggc gcccctgtca gcctgccgct
    12601 atggcgaggg cgccggtcag acgcggatga tcaggcacga ccaggccggc agccctgccc
    12661 gcccggcgcc acctgcggca tggaggatct cgccctcggg caaaggcggg gccagcgggg
    12721 ggccgcccag gttgaaggca cagcacagca cctcgccggc cagcgcccgc tcgaacacca
    12781 gcagcgcgcc ttctgcccgc agcagccgca agcccccctg cgccagcgcc ggaaagcttt
    12841 tgcgcaaggc gagcagcgca cgggtgtgat gcagcagcga tccggcatcg gcctcttggc
    12901 ccgccaccgc gcgggccaca ttggcgggcg acagcggcag ccacggcgcg ccggtggtga
    12961 agcccgcctg cggcagggcg gcatcccagg gcatgggcgt gcgcacgccg tcgcgcgtca
    13021 gcgtcagcgg ccagttggcg atcgcctcgg ggtctttcag cagggcgaag gggatctcgt
    13081 cctgcgtcag ccccagctcc tcgccctgga acaggatgac attgccgcgc agcgccagca
    13141 gcagcgccat cttcacccgg aggaaggcgg ccacggcccc ctcatcggca tcgcccgcca
    13201 gccagcgcca gctggcccga ctggaatcat ggttctcgaa agcccagctc ggccagccct
    13261 cggccgggcc atgcccgccc cttgcagcgc caggccagga cgtcagcgcc tctgccacca
    13321 ccgctgcatc cagttgccgg gcatagagga aattgaagcc ataggcgctg ttcagccggt
    13381 gatcgccggc ggtataggcc ttcatctcct cctcggcgcg atcgccgccc acttcggcca
    13441 tggtgaagat cgcccggtag ccatcgcaca gcgcgcggat cttctcgaca aagccgacca
    13501 cgccgggatg cgacatcgag cggctgtgct cctgaaagtc atacgagcgg gtgcgcagcc
    13561 ggccgtcgtc aaccgccacc ggattgtcgc gcaggtccgg gtcgaacatg gaatggttga
    13621 gcgcatcgag ccgcaagcca tccacccccc gctccagcca gaagcgcagg gcatccagca
    13681 gggcctcctg cactgccgga ttgtgcaggt tcagctgggg ctgctccttc aggaactggt
    13741 gcatgtaata ttggcggcgg cgcgcgtccc atgtccaggc ggggccgccg aacaccgatt
    13801 gccagttgtt gggcggcgtg ccatcggccc gggggttggc ccagacatac cagtccgcct
    13861 gcgcgcctgt ggcatcgtgg cggctggcgg tgaaccaggg gtgctggtcc gaggtgtggg
    13921 cgaagaccat gtcgatcatc accttcagcc ccagctcatg ggcgcgggcc accagcgcgt
    13981 cgaaatccgc cagcgtgccg aagatcggat cgacatcgca ataatcggcc acgtcatagc
    14041 cgaaatcggc catcgggctg cggaagaagg ggctgatcca caccgcatcc acccccagcc
    14101 gggcggcatg gtcgagccgg gcggcaatgc cggccagatc gccgatgcca tcgccgttgc
    14161 tgtcgacaaa gctgcgcgga tagatctggt agatcaccgc gccgcgccac cattcctctc
    14221 ccgcgccggc gccagcctcg aacagggggg cctcggacaa gggggcaatc ggctttcctg
    14281 tcattgtgtc aacagctccg gggcaggcgg ggcccctgtt atcaccgcca gaggccgggc
    14341 cgtcaaagca aagtggatcg gctttgtgcc ggcggcgcag gcggcgggca ggcgcacacg
    14401 catgctacgg aaaaaattga aaatgcgtcg caaaaagccg agcaaatgat acacaaagtt
    14461 taccattttg ctctcctata tgcgccacag gggcagcagg gtggcaacaa aggtggtaac
    14521 caaggcggca gccaggatca gacgcttttc ggccgcctgc atcattgcct gcgtggggct
    14581 gatggtggtc atctcgatcg tggtggcagc ggccctggcc catcgccagg cgatagaggc
    14641 gcgccaggcc tatgcgcagc agctggcccg ccaggcgctg gccagttccg aaagaaccgc
    14701 cgacgagctg gcccaggcca ggccgctgat cgctgccacc agcgcccgca ccggcgcccg
    14761 caaggcctgc tccgatgccg atatcgagac catgcgcggc atcgatctgg cctcgccgct
    14821 gctgcaggcg gtggggcgga tagagggcaa cacgatcctg tgctcctcca tcgccgggca
    14881 gcgccgcatc gatctgggcc cgcccgatct ggtatcgcgc gcaggggccc gggtgtggct
    14941 caatgtgcgg cccatgggcg gccagcgcag ctatctggcg attggcagcg gggcgctggt
    15001 gggcatcatc cacaaggatc tggcgctcag catggtggag ctgatgccgg gggcggcggt
    15061 cagcagtttc aactggtcgc acaaggtgat gctggccgcg cgcgggccct ttgccccggc
    15121 ctggctgcgc gcggatctgg ccggcggcag cagctggcgc gtgcatggct tcgtgctggc
    15181 ggtggcccgt tcgcagcgct atgatctggg cgtggtggca gcagccccgc taaatgccgt
    15241 gcctcgcctg ccgctgcaca ccgccgccct gctgctgccg atcgacatat tggccggcct
    15301 gctgctgacc atggccatca cccgcatcgc cgaggccaag gcatcgctgc ccgccatgat
    15361 tgccggcgcc atgaagcgcg gcgaattctt catcacctat cagccgatcg tggcgctgga
    15421 gagcggccgc acggtgggcg ccgaggcgct gatccgctgg cgccgcccca atggcgaggt
    15481 gatcgcgccg gatctgttca ttcccgtggc cgagcaggcc ggcagcatcc gccatctcac
    15541 cgcccatgtg ctggatctgg tggcgcgcga tatcggcgcg catatcccca cggccgaact
    15601 gtcgcacctg tcggtcaatt tctcggccga cgacatgcaa tcgcccgaca tggtggaaaa
    15661 tatcctcgcc ttctcgcgcc aggcgggcat cgccatggcc aatatcgtga tcgaggcaac
    15721 cgagcgcagc ctgatcgaca tcgagctgtc gagccaggcc atccgccggc tgcgcgccat
    15781 gggcgtgcgc atcgccatcg acgatttcgg caccggctat tccagccttg gctatctggc
    15841 ccagctggaa atcgacatgc tgaagatcga ccgcctgttc gtgcaggcgc tgggcaccgg
    15901 ctctgcggcc agccaggtgg ccgagcgggt gatcgagatg gcccgcgacc tcaagctgga
    15961 aatcattgcc gagggcgtgg aaacgcgcgg ccagatcgag cggctgaaga cgctgcaggt
    16021 ggatctggcg cagggctatt atttcgcctt tccgatgccc atcggcgagc tgcagaagcg
    16081 cctgcgcaca gaggccggcc tggcgcccgt ggagatggcg cccgtggaga tggcgccaga
    16141 caccgctccc cggctacacg ccggcgcccg ctgaagcgct gcccggctgt aggaacacca
    16201 gccggaaatc gccgcgttcc acttcggcca gcgtgtgctg ggtgaaggcc agcggcccat
    16261 ccaccgggtg gcagaagctg cgctcgccgc cctcgcgggc gatcacgctc tggcgctccc
    16321 agccggcgcg gaacgcctcg ctgtgcaggc gcagccaggc caccacgccg cgcacctgcg
    16381 gatcgcccgg ggcccggcca taatcgcggc gaaactcggc cagcacccgg gcgctgcggc
    16441 cgggccaatc gggcagcagc gcgcgggcgg ccggatgggt gaagatatag cgcagcaggt
    16501 tgggcgcctc ctccccctcg aacaggccca cgaacagccg ccgcgccgcc gcattggccg
    16561 cgcacaccgt ccacgcccga tcaagcccat aggccggcca ggccagcgcc gccaccgccg
    16621 cggcaatcgc cgggggcgcg gcatggcggg cggcggcaaa gggatcggcc ggatcgcccc
    16681 tgccggccag ggtgaacaga taggcccgct cggccgctgt caggcacagg ccatcggcca
    16741 gccgcgccag cgtctcggcc gaggcgcgca ccgcccggcc ctgctctatc cacgccaccc
    16801 aggtgctgcc gatgccggcc cgcgcggcca gctcctcgcg ccgcaggccg ggcgtgcggc
    16861 ggcgccctgc gggctgatcg ggggcgatgc tttccctgcg ggcccgcacg aaggcgccca
    16921 gcaggcggcg ttgttcctca tctggcatgg gggttcttat aacaggataa ctttgtccat
    16981 tgtaacaggc tttatccagc gccacaccgc ccttgcccga aaaaagcgcc ggcaccccgg
    17041 caaggagaca gccatgaccc gcacccatga aaccctggtc gatcagcaat tcggcgcgcg
    17101 tgccgccgcc tatgtcgcca gcgccgtgca tgccagcggc gccgatctgg cgcgcatggc
    17161 cgccatcgcg gccgcccatg ccccggccca tgcgctggat ctgggctgcg ggggcggcca
    17221 tgtcagctat gccatcgccc cttatgcgcg ggcggtggcc gcctgcgatc tggcgccgca
    17281 gatgctggcg gccgtggcgg ccgaagcgca ggcgcgcggc gcccaccata tcgccaccca
    17341 tgccgccgcg gccgaggcgc tgccctttgc cgatggcgca ttcgacttcc tcgcctgccg
    17401 tttttccgcc caccactggg ccgatgtgcg ccagggcctg gccgaggcgc ggcgcgtgct
    17461 gcggccgggc gcgccggcgg tcttcgtcga tgtgatcgcg cccgaagcac cggccgccga
    17521 cacccatctg caagcggtgg aactgctgcg cgatccttcc catgtgcgcg attatcgccg
    17581 cagcgaatgg cgggcgatgg tggccgaggc cggctttgcc ataaccgccg agacgcccgc
    17641 gcgcctgcgc atggattttg ccagctggac cgcgcggatg gcaacgcctg ccccccacat
    17701 cgccgccatc cgcagcctgc agggcgcggc cagccacgat gtcgccggcc atttcgccat
    17761 cgaggcagat ggctcgttca ccatcgacac attgctgatc gagctgcgct aaggaggcgg
    17821 ccccaacatc cgacaaagat caggtcacaa accggcggcg ccctttatcc tgccgccccg
    17881 caagcggcgc cggcatcccc cggcccgccg cgggagagag catatgccac gttttaccga
    17941 caagaccgtc atcatcaccg gcgccgcctc ggggctgggg ctggccgcgg cgcacaggct
    18001 ggcggcagaa ggcgcccgcc tgctgctggt ggacaggaac gcggccgcgc tggaagcggc
    18061 catcgccacc ctgccggccg gcagcgaggc ggcggcgcat atcgccgatg tggcccgcca
    18121 ggaagaggtg gaagcctatg tcgcccgcgc catggagctg tggggccgga tcgacggttt
    18181 cttcaacaat gccggcatcg agggccgcca gaacctcacc gagaatttcg gcgccgagga
    18241 attccaccgg gtcgtctcga tcaacctcga tggcgtgttc ttcggcatga tggcggtgct
    18301 gaaggtgatg cgcgcgcaag ggcatggcgc catcgtcaac accgcctctg tcggcggcat
    18361 ccgcggcgtt ggcaatcaga gcggctatgc cgccagcaag catggcgtgg tgggcctgac
    18421 gcgcaacagc gcggtggaat atggccagtt cggcgtgcag atcaacgcca tcgcccccgg
    18481 cgccatcatg accgcgatgg tggaaggatc gctgcgccag atcggcggcg acgattggga
    18541 ggaagtgggc cgccagttcg tctcggccaa cccgatgaag cgctttggcc gggtggagga
    18601 agtggccgcg ctggtcgcct tcctgctgtc gggcgaggcc ctgttcatca atggcgaggt
    18661 gatcgccatc gatggcgggc aatcgggcaa atattgatcc cgaaccgggc ggctgcgcga
    18721 gggaggctcc ccccgcgcag ccccgccttc aggccagatt gttgcgataa agcgtggtgc
    18781 ccgccttcca cgagacgccc acctcgtgcc agtagccggc gatcttctcg acgggcgcaa
    18841 ggtccagcag ctcttccacc ggcgagggga aataggccag atcgaaatcg ccggtgaagc
    18901 agcggccgaa atcgacatca tagcccgcca tcgtcaccac ctcgtcgagg ctctgggtgc
    18961 cgtcggtctc gatggtgggc atccagcggt tgtgggccat ggggtggccg ttgacgaagc
    19021 cgggcttttc agccgcgccg gtgatggtga agcgcccctc ggccaggcgc cggtcgaacg
    19081 cggccagcgt cacgccgaaa gtgccgcccg gctgcagccg cggccccgcc ttgccgacat
    19141 agaccggccg gctcatctgg atgctgccca gcttcttggg atagccctga tgctggccgc
    19201 gcagcatggc aaattccttg tccacccaga tgtagacgca gcgcgaatag gtcttgccct
    19261 ggaactggca gcgcaccacg atgaacatct ctttgtattg cacgcggatg ggatcgagga
    19321 tctgggccgg gtcatcgctg gtcgattgcc agtcggccca gatggcggcc accgcgcccg
    19381 ggtcttcctc ggccagggtg aagcctgcgg gcagcagggc cgccaccttg gccggatcgg
    19441 tgcgatattc cagcgtgatc agctcgccgg aatagtgcca gggcaccggc ggcacgatgc
    19501 tggcctggcc gctgggcgtg cgcggataga acaatccctt cagttcgctc atactgtcct
    19561 cgaaactggc ctcgaaatag gctgcttggc gcccatcatg gcgcaggcgg caaggcgcat
    19621 cttggccgca acggccaaag ccagcgcgca cgcgcagcgc tacaccccaa ggccagcatt
    19681 gccctttgag gaaccgcccc atgaccaccg aataccgccg catcctgctc gatggctatc
    19741 ccatcctcac cacccgcgaa ggcgaccggc tggtgacgaa ggacgggcgc agcatcgcca
    19801 tcgacgaggc gatccacctg ccgccggtga acccgcagaa ggtgatctgc gtgcacctca
    19861 actatcacag ccgcgtcaac gaattcatga ccaggctgcc tgccgccccc acctatttcc
    19921 acaagccgat cagcgcgctc aacagccaca agggccatgt ggtgcggccc gagcgctgcc
    19981 agttcctcaa ctatgagggc gagatcgcca tcgtcatcgg caagacctgc cgcaatgtga
    20041 gccccgaaga agcgggcgat tatatcctcg gctatgccgt ggccaatgat tatggcctgc
    20101 atgatttccg cgataccgac agcggctcga tgctgcgggt gaagggctcc gacacgctgt
    20161 gcccgatcgg gccgggcctt gtcaccggct gggatttccg caacaaggcg atccgcacct
    20221 atgtcaatgg cgagctgaag caggacggca ataccgccga catggaatgg gacatgcact
    20281 atctggtggc cgacattgcc cgcaccatca cgctggagcc gggcgatgtg ctgctgtcgg
    20341 gcacgccggc cggcagccgg ccagtgcagc cgggcgatct ggtggaagtg gaagtggaag
    20401 ggctgggccg ccttgccaac accatcgtcc acggccccac ccccatccgc gacgattgcg
    20461 gcgcccagcc gaccgagagc gaggaagtga tctccaccgc gatgggcggc gactgggaat
    20521 ttcgcggcca gcgcgcagcg cgcgggcagg gcgcggcctt ctatgaatcc actgtcgagg
    20581 aaaagtgacg aaccatgggt tttgacatga cgacgggcgc ggcgcgcttc tacatcggcg
    20641 ggcgctgggt ggcgccggcc agcggcgcaa gcgcgcccat cgtcaacccg gccacagggg
    20701 ccgagatcgg ccgcgtggcg ctgggcggca gggccgatgc cgaagccgcc atcgccgcgg
    20761 ccagggccgc cttccccggc tggagcgcca ccagccggga agagcgcctg gctttcctcg
    20821 aaaagatcca cgccggcctc atcgcccgca gcgatgagat cggcgctgcc atcagcgccg
    20881 agatgggcgc ccccggctgg ctgggccgcg ggccccaggc cggctctggc ccgcagcatt
    20941 ttgccgaagt gatccgcgtg ctgcgcgatt atcccttcga gcagccgctg ggtggcacgc
    21001 tgatccgccg cgagccgatc ggcgtgtgca cccttatcac cccgtggaac tggccgatga
    21061 accagatcgc caccaaggtg gccccggcgc tggcggccgg ctgcaccatg gtgctgaagc
    21121 ccagcgagct ggcgccgctg gatgccgcga tcctggccga gatcatcgat gcagcgggcc
    21181 tgccggcggg cgtgttcaac ctggtgcacg gcaccggcgc ggatttgggc gaggtgctta
    21241 ccgcccatgg tgatgtcgat atggtcagct tcaccggctc cacccgggcg ggcattgcca
    21301 tcggccagca ggcggccccc acgatcaagc gggtggcgct ggagctcggc ggcaaatcgg
    21361 ccaacatcat ccttgccggc gcgccgctgg accaggccat tcccgccgcc gtgcgcggct
    21421 gcatgctgaa cagcgggcaa agctgcaacg cgcccacccg cctgctggtg ccccgcgccg
    21481 atcttgatgc ggccgtggcg ctggccagaa ccgcggccga agcgctgacc atcggcgcgc
    21541 ccggggccga ggcgaacctg ggccctgtgg ccaatgctgc gcagcatgag cgggtgcgcg
    21601 cccatatcac ccgcgccatc gccgaggggc agacgctggt gaccggcggc cccgaggcgc
    21661 ccgcggggct ggaacagggc tatttcatcg cgccgacgat cttcatcgcc acgcccgatg
    21721 ccgccatcgc ccgcgaggaa gtgttcggcc cggtgctggc catcctggcc tacgacacgc
    21781 tggaagaagc catcgccatc gccaacgaca gcccctatgg cctgtcgggc tatgtgtggg
    21841 gcgcaacgcc ggcagaagcg cgcgatgtgg cgcgcgccct gcgcaccggc atggtgcatg
    21901 tgaacggcgc cgggctcgac acggcggccc ccttcggcgg ctacaagatg agcggcaacg
    21961 ggcgcgaatg gggcgtctac gggctggagg aattcctcga ggtcaaggcg atctacggcg
    22021 gcgcctgagc gcccttgcga ggggctgccg gcaagctaga ctggcgccat ggcagccccc
    22081 accatctcgg cccagttcct gcgccacgcg gccaattgcg tggagctggc cggccatgcc
    22141 cccgatccgc tgctggcccg gctggggctc gaccggcgcc agctggaaga ggccgagcac
    22201 gacatccccc tcagcgcctt cctcgccttt ttcgaacagg ccgcgcaagc cgtgggcaac
    22261 ccccatttcg gcctctatgc cgggcgcatg gtgggggcag acagcctggg catattgagc
    22321 ttcctgttcc tttccgcgcc caccctgcgc gaggccttcg ccagcttcac ccgctatctg
    22381 gaaaccatgc agcaggcctc gcgcaatgat ttcgtggaag ggcggcgctg gggccgcttc
    22441 cagtatggca tcatcgacga cacgctggcc gaacgccgcc aggatgccga atattccatc
    22501 ggcgccacct acacgctggc ccgccaatat accggcggcg cgctgagcct ggaggaagtg
    22561 cgcttcgaac acccgctggt gggcgattat cgccgctacg aagcctattt cggctgcgag
    22621 gtgcatttcg ggcaggacag gaattccttc tcctttgccc gctccttcct cgattttccc
    22681 ggcaagcagc tgagcgccac gctgttcccg gtgctggtgg aacacctcag ccgccgccgc
    22741 gccgccgagg ccacccccga cagccgcgcc gcccaggtgc gcgcgctgat cgccgccgcc
    22801 ccgctcgacc gcccccccga tgcccccgcc cttgcccgcg cgctgggcat ttcggtggca
    22861 acgctgggcc gccagctgcg cgcggaagac tgctctgtgc gcgatctggt ggccgcccgg
    22921 cgcatgcagg ccgcccgccg cctgctgcgc gatggccagc gcagcatcgc cgatgtggcg
    22981 atcgccaccg gctatgccga aagcgccagc ttcatccgcg ccttccgccg ccatttcggc
    23041 gcagcgccag gccatgtgcg gcgccaggga taggctggcc cccacgccag ccctgcgcgc
    23101 aaaaaaacgg gcggaaagcc gcagcttttc cgcccgcatc aggttcaggg ttcggtcaaa
    23161 gggattaata tttcgcggta atgctcacgc cccaggtgcg cgggcgggca taggtctggt
    23221 tcgaggcgcc cagcaggctg gaaagatcga ggttgtagac ccggtaccac ttgtcggtga
    23281 cattgttgac gaagccggcc agctcatatt tgccatcggc cagcgcatag ctcacccgca
    23341 cattgcccac cgtgtggccg ggctcgaaat cgacaggggc gttgttcgtc tcgaaatatt
    23401 cgcccgaatc atacttccag tcggtggaga tggccagctt gcccggccct gcggcaaatt
    23461 cataccgcgc ctgcccgccg atcgaggtct tgggggcctg cggcatcacc cggtcggcca
    23521 cggtgccatc gggcagggtg atgttcttca ccttggtcga cagcagcgtg aggaaggtgc
    23581 ccagataaag cccgcgcacc ggccgcgtgt tgaactcgaa ttcggcgcca tgctcatagg
    23641 cctggtggtt cgagatatac tggttcacgc cgacattctg gaaggcctga tagccctggt
    23701 agttatagaa gaacaccgag ccattgaaat gggtggtgcg gttcagcatg gtcagcttga
    23761 agccgccttc ccagttggtc agcacttcct ggccaaaggg gatcgcctgc accagcggat
    23821 agccaaggct ggaggaaacc gcggcgctgt cggtataggg cgtgttcgag gaaacggtgc
    23881 cgaagccgcc cgacttggtg ccacggttga cgctggcata gagcagcgtt gcattgttcg
    23941 gcttgtagtc gatctcggcc ttgcccgacc agttgtcgaa ggtgcggaac cgcagggccg
    24001 ccaccgaggg gatcgaggtc gcccagtcga acatcagctc gccattggca taatcggcaa
    24061 attcgatgtg cttccgatca tgcgaatagc gcaggccgcc aatcaccgac accttgggcg
    24121 tgatcgcata ttccagctgg gcgaaggtgg ccagcgagaa ggtgttctgc gtgccgccat
    24181 aatcgagcag gccgatgccg gggatggcgg ttacggggct gttgatctga tagtgattgg
    24241 cggtgtggat atcgagcaga tagacgccgc ccacccagtt cagccgctcc gtcttgcccg
    24301 acaggcgcag ctcttccgac atctggtaca tgtgctggtg ggtgtcgtag ttgaaggcgt
    24361 tgttcgggct catgtccgaa tcctcgccat aatccttgtg caggtgctgg tagtcgctga
    24421 tcgaggtcag gtcgaaggcg ccgaaatcat gactgtagcg cgcgctgaca tcccaatagg
    24481 tgcggttgaa gctgcccacc cggtcttccg actggatgaa gggattgccc gatgtgttgc
    24541 tgtagccatt ggcatcggtg ctggtgaagg tgccaaggcc gtggttgttg gggtcggcat
    24601 agctggcgcc ccagctgtag aggccgccgg aacgctcatg gtcgttgcgc agcgtctgca
    24661 ccttgatgtt cagcttgtca cgcggggaaa gatcggcgct gatctggccg cgcagggcat
    24721 agaacttggc attgccaagg ctgggcccca tggtgttgcg gatatagccg ccggcgttgt
    24781 tggtagtgaa gctgaagcgg gcgcgcagcg catcggtgac agcgccgctg accgcgcctt
    24841 cggttgccac ctgcccgtag gagccggtgg tgagggtgag atagccctcg ggccgggcgg
    24901 tgggctttgc cgagacgaac tgcaccaggc cgcctgtggc attgcggcca aacagcgtgc
    24961 cctggggccc gcgcagcact tccacgcgct ccagatcgaa attcatgccc gaaatggcgc
    25021 ccagcgaggc gacatagacc tcgtcgtcgt agaaggcgat gggggcttcc tgcgcatcgg
    25081 taaagtcgtt ctgcgagacg ccgcggatgt tgaacacggt gatcgtcggc gcatactggt
    25141 tgacttcgag gttgggcacc tggccggcca gcgcggtcac atcctgtcgg ccgatggccg
    25201 cgatcgtgtc cttgccaagg gcggtgatgg caatgcccac cttctgcaca ttctgctcgc
    25261 gcttctgcgc cgtcacgacg atctcgccgg gggcggtagc ggccggggca ggcgcggcct
    25321 cgtcggcgcg ggccatttgc ggcagggccg ccgccagcgc cagcacggag acgcaacgca
    25381 cggatttccc gatcttcgac atgtccgatt aactccccga aatgctcttt gctcggcccc
    25441 tcatggccgg tcttgctggc cgcatagtgc ggcggggcgg gcgccggctc ttgccatttg
    25501 cggccatttg gcgcggcggg gcgggccgcc ccttggccgc tgcggccatt ttgcggcgcc
    25561 cttgccctca cccccgcgcc agcaggcccc ggtgccggcg gatgggcagg cgcagcaggg
    25621 catagctggc caggaacagg cccacgcaca gcagttccac cggcagatag ccatggccat
    25681 ccagcagcag cgcggccagc atcggcccgc cgcccagccc cgtcatctgc atggcgatgg
    25741 caaagcccat gatccgcccg ctctcgtcga aatcgcagat ccgcccgagg atgaagggca
    25801 gcacgaaatt ccacagcacg ttgaaaccgc aaaccgccag agtgaacagc cccacccccg
    25861 ggtggcccac cagcagggca atgctggccg cccccaccaa tatgccaaag gcgatggcat
    25921 gatcgcggtt gatccgcgcg gccaggaaca ccgaggtcag cgcgccgaaa attgccagga
    25981 tctggctgag gaacagggca ttggcgatct gctggtcggc aaggccggcg ccggcgccca
    26041 ccagcgccag gatcgcccag gcaatgccct gcgccagatt ataggaaagc acgcccgcca
    26101 gcgccaccac cagcagcgcc agcggcaatt gccgcgcctc ggggttggcc atgtcggcag
    26161 cggaaaagcg gtggggcagc aagggcagca gcagcagcgc ggccagcgac aggccggcaa
    26221 aaccggcaaa cagcaccggc aggccatacg catcgagaaa acgcggcagg aaccacaggc
    26281 caaaggcgcc ataggtgagc agcagcgaga gatagagcgc cagattgcgc tctgcccgca
    26341 aggtgatgcc cacaaaggtg aagctgatcg agatgatcac cccttcgcca aggccggcca
    26401 gcacgcgcgc cggcgcaaag ccggcactgc ccgtgctgag cgccgaggcg gcatcgccgg
    26461 cggccgccat caacagccct gcggccacca cccagcgcca gccgatgcgc ccgcccagca
    26521 gcgccagccc caccgtggcc agcgccacgc ccagcatctc cagcccgacg agattgaccg
    26581 cctgcgcctc gctgaccgcc agatggcgca cgaaaccttc caccagcccg ggctggatga
    26641 tgaacgagag caccccgatc gtgcccagca cgatggcgct ggccaccgcc ggccagccat
    26701 tgacgtctac ttcgtggaaa ccagcctctt gccgtgcgct cattgccctc atgcccttat
    26761 tttggtgccg gcatcctctc atggggcctg cgctgccagc ttggccgcag cggacaattg
    26821 atcctgccgc ttgaccgggg tcaatcggca aggggcgggg cgcggtttgg tgatcgaaac
    26881 tgtcaatcca tgcggtgttc ggtcaatatg attcggcccc tctcggttac tcataagcaa
    26941 ggagaggaaa acaagatctc ggggacggaa gatgacaaat gctgccgtga gagtggccgc
    27001 cttggacggc tgggccggtg tgctcgccaa caagggtatc aatgccagcg atgtgatgag
    27061 cccggcgcca gaccaggccc cttgcgccac cgacagcgcg atgatgccgc tgaaggattt
    27121 cgtgcgcttt tcagaagcgg tggtggccca cagccgcgat gccgccttgc cctggctggc
    27181 cggcacccat tacgacctgt cgtcgctggg ctcggtgggt gatgccatca acaccgccgg
    27241 caaggtgggc tcggccatcc gccgctttgt cgaatatttc agcctgctgc aggattgcac
    27301 cgatatccac ttcggcctcg aagggccgat ggcctgcgtc agctatcgca tcctcgatcc
    27361 cgatatctgg ccgcgccact atgacgcgat gttcagcctg ggcatcatcg gccagctgat
    27421 ccgccgcggc cgccatggcg gctgggacaa ggtggaattc gccttcgagg ccgagcaggg
    27481 cgaattgcgc agcaacatcg cccaggtggt gcaggccccc tgcacctttg gcgccgacac
    27541 caaccagatc cgctttcccg cagcctttct cgatctgccg ctgccggtgg cgcgccgggc
    27601 cgacgacatg cgcccgctgg gccgcgcgct ggtggccaag cgccgcgcca cgccggtgag
    27661 cgagcggctg gcccagctgg tgttccgcga tctcaacgcg ctggcgatcg accaggaccg
    27721 catcgcccgc gagatcggca tgtccacccg caccatgcgc cgcaagctgg ccgaggaggg
    27781 cagctcctac cagcaggtgc tcgacgaatg ccggatgcgc caggccctgt tcgagtttcg
    27841 cacccggccc gatctttcca tcgcccagat cgccctgcgg ctgggctatg ccgaacacag
    27901 caatttcacc cgcgcctttc accgctggag cggccagtcg ccccaggcct atcgcgccag
    27961 cctggcccat ccgcagcact gaacggcgcc acagcaacgg aagcgataca acccatgacc
    28021 agactgctcc tgctggaagg caacaccgcc gacaagcgcg cccttggccg ccagctgggt
    28081 gtgcgcagct ccagcgagat ctacgccgag gcgatcgccg cccattaccc cgccttcgaa
    28141 ctggatgtgc tgaacggcgc cgatgccgat ggcgccatcc cccacggccg cagctggagc
    28201 gattatgatg gctttgtcat caccggctcc tcgctgcatg cctatgacac cgattttgcc
    28261 gtgaccaacc agatcgagct gacccggcag gccgcgcgcg cggggctgcc ggtgttcggc
    28321 tcgtgctggg ggctgcagat tgcggtgatg gcggccggcg gccttgtcgc ccgcaatccg
    28381 gccgggcgcg aggtgggctt tgcccgcaag atctggcgca cgccggccgg cgccagccac
    28441 ccgatctttg ccggcaaggc gcccgtcttc gatgcgccct gcatccatta cgacgaagtg
    28501 gtgcgcctgc cggaagcggc aacgctgctc gcctccaacg cgcatagcct ggtgcaggcg
    28561 gcggtgatcc cgctggaggc gagcgaggtg tgggcggtgc aataccaccc cgagttcgac
    28621 ctgctccagc tggtgcagct ctacacgctt tatgccgaag acatgatcgc ccagggcttc
    28681 ttcgcagacg aggccgagct ggtggcctat cgcggcaaga tcgccgcgct ggcggcaaat
    28741 cccgataaag caggccttgc ctggcagctg ggcgtggatg ccgatgtgct ggacgatacc
    28801 cagcgccgcg gcgagatcct ggcctggatc gaggcgaaag tgctgggcaa caggtgattg
    28861 gaaggcaggg ggcgaaggtc aacaccctcg ccccctgccg gccttaccag ccgccttcgg
    28921 gccggtcgaa ccacacatgc acctggctgg tgccagtggc gttctcgtaa tcggagaaca
    28981 gccggccctt ggccgtccat tcccgcccgc caagcgctgc tgccatcatc agataatggc
    29041 caaagcgcgc ctcgggggcg tggcgcagat attggggcat gctgtcgatc acggcggcgt
    29101 ggttgccttc ctggaaccag gcgatccgtt ccagatcggc ctcgcgcgcc tcgggggtgg
    29161 agatgtggat ggggtctgag gcctcatgcg cggggatcag ccgcagcggc cagaagcggt
    29221 ggctcatcga gcccgaggcg atcagcacca cgcgcttgtc ggaagcgcgg atggcctcgc
    29281 caatgcccgc gcccactttc aggaaatctt ccggatcggc cgaataggca tgcgacacgc
    29341 caatccattc ctcgccgcga tcgaggaaag tggaaaggtt caccgtggga tagtggatcg
    29401 gcagccaggg atcgtcgatc ggcgtggtgc ggatgccgtg gcgggtggca gcggcggcca
    29461 tttcggtggc cagctgcggg ttgcccttca gatcataggg gatctgcttc atgccgcgcg
    29521 gcaactcgtc ggaggtgtag agccccgagc ggcgcgcatg ggccgagacg acgaattcga
    29581 cgatagtgaa ccagtgcgtg tcgaagatga cgatggtgtc gggcttcagc gcgtccagca
    29641 cttcgctgcg caggcgccgc agccccggca ccaggctgat ctccttgccc tcgttcagtt
    29701 catagcgatc ctgctcggac atcatgatgg tcggcgcatg cgcgacgatg ccggctccga
    29761 tgatctcacc cataacagcg gtgttccttc cacacattgg tggaagccca catacgcgca
    29821 gggcccgcca tccggaacag cccgccccca gcttggccgc aactgccaag tcgcccggcc
    29881 gccctgaggc ttagcaccgc cccaaggaca aaggaggata tgatgcgctt tgcattctcg
    29941 cgctcgaacg atgcccatgt tgatctttca tcgctcgacg cgttcaacga aggcgcgccc
    30001 tttgccacct tcgatcgcct gcgccgcgaa gaccccatgg cctggagcga tatggccaat
    30061 ggcgaccgcg gcttctggtc tgtcacccgc catgccgacc tgctggagct gaaccgccag
    30121 gcggacctgc tctcttctgc ccgcggcatt cgcatggaag accagagcca ggaggaatac
    30181 gaggcccgca aaaccttcca ggaaaccgat gcccccatgc accgcgcctt tcgcgcgctg
    30241 gtggccaagg ctttcgccaa gcacacggtg gcccagtatg aggagcagat ccgcgcgatc
    30301 gtgaccgacc tgctggatgt ggcgctggaa gagggcactt tcgacgcggt cgaccgcatc
    30361 gcccggcgcc tgccgatgca gatgctggcg cagatcatgg gcgtgcccac cgaggatggc
    30421 ccctggctgg tggagaaggg cgatgcgctg atctccaatg ccgaccccga ctataccgat
    30481 ttcgtcgtcg acaaggtgga taccgaggcc taccgcctgc tgcccttccg ctcgccggcg
    30541 gcggtggagc tgttcgatta tgccaacagc ctgctcgccc gcatggacaa gggcgaacag
    30601 atcggcgtgc tgaacctggt gctgcaggaa accagccacg gcacttccat gagccgcgat
    30661 gaattccgca atttcttctg cctgctggtg gccgccggca acgacaccac ccgctattcg
    30721 atctcggcca cgatccacgc cctggccaac aacccgcatc tgctggcgca gctgaaggcg
    30781 ggccagttcc tgggctggga agaggcggcc gacgagatga tccgctatgc ctcgcccacc
    30841 agccatttcc gccgcaccgc cacgcgtgat ttcaccttcc atggcaagga tgtgaaggcc
    30901 ggcgacaagg tgctgctctg gttcctctcg ggcaatcgcg acgagacggc gatcgaggat
    30961 ccctatgcca tccggctgga ccggccgcgc aaccccttcc tctccttcgg ccagggcggc
    31021 ccgcacattt gcctgggcat gtggctggcc aagctggaag tggcgatcgt tctgcaggag
    31081 ctggccaagc gcatcgagca tatcgagcag gccgccgagc attcctatct gcgttccaat
    31141 ttcatccacg gcatcaagca cctgccggtg cgcgtgcgcg cggcctgacc ccctttcagc
    31201 tgaaagcccg ccttcccatg accatccagc accccttcga tgcccatgcc gccgcctttg
    31261 cgccgatcga gcgctgccgc ctgctgtttg ccgaccagct gaacctggcg cgcggcaaat
    31321 atgtgccgat gtcggaagtg ggcaagggcc acgcccggct ctgcgttggc acctatgccg
    31381 tcacctacga caagcagatg gtggcagcgc cgggcggcgg cgtgctcgat ggcctgcccg
    31441 atatcgagct gcgcttcgac cccaccgccc tgcgccccgg ctgggagccg ggcacgcggg
    31501 tggcgctggg cgatctggcc tacaagggcg cgccctttgc cctgtgcggg cgcagcgcgc
    31561 tgaagcaggc gatcgcggcc tggcgcgagc gggggctgga gccgatggtg ggcatcgaga
    31621 tggaggccta tgtgttccag cgcggggccg atggcagctg ggtgccttat gacacgccgg
    31681 gcgccttcgt ctatggcacc gggccgttca ccgatccggc cggccttgtc gatgatatct
    31741 ggcaggcagc gcagcattgc ggcctgccga tcgaatcgat caacgccgag ttcgatgccc
    31801 cccagttcga gctgaccctg cgctatgccg atgcgctgag cgccgccgac gacagcttcc
    31861 tgttccgcca gatggcccgc gaaatcctct acaagcgcgg ctatctgctc tccttcctgc
    31921 cgcgcccgat cgccggcaaa agcggctcgg gcctgcattt caacctctcc ttcaccgatc
    31981 cgcaggggcg caacgtgttc gcgcaggatg tggtgaacgg gccgggcgag gtaatgagcg
    32041 gcgccatcgc cggcctcctt caccaccacg aggcgctggc cggcatcctc gccccgctca
    32101 ccaattccta tgagcggctg cagccggcca gcctgtcggg ctattgggcg aactggggga
    32161 tcgaccatcg ctccaccacc gtgcgcgtct cggccgaaac cggccaggcc gcccgcatcg
    32221 agcaccgcat ggccgatggc gcggccagcc cctatttcgc cctggccgcc attttgcgcg
    32281 cggcccttct ggggctggag cacggctatg ccctgccgcc ggtggaaacc cgcgacgggc
    32341 tggaagaaac cggcaccagc cgccacacgc cccatgatct ggccagcgcc ctcgatgcgc
    32401 tggccgccga caccgagctg accagcgcca tcggcgccga actggtcgac aattacctga
    32461 cgatcaagcg caccgaagtg gccgacctgg ccggcaaaag cttcgacgag caggtcgcct
    32521 actacctcca ctacatctga ggaccgccat gagctcgccc cgcatcgaca tcgaaggcat
    32581 tgccgtctcg cccgatcatt tcatcggcgg cgagcgggtg gcctcgccca ccacattcga
    32641 aacccgctgc ccgttcgact gggagcgcaa gctggccgac atcgcccgcg gcgatgccga
    32701 caccgccgcc ttggccgccg ccgctgccac ccaggccttc cccgcctggg ccgcgctcag
    32761 cccggccgag cgcggcgcct atctgcaccg cctggccgat ctcatcgagg ccaatgtgga
    32821 gaagctggcg ctgatcgaat gcctcgacat gggcatgctg catgaaagcc tgcgcctgcg
    32881 cgttatcctg cggggggcgg ccaatttccg caattatgcc gatctggcca gtgcccatga
    32941 agagcgcgtg tggtcctcgc gcggcacgct gaaccgggtg atccgcatgc cggcggggcc
    33001 atcgctgatc atcaccccgt ggaacgcgcc cttcatgctc tccacctgga aatgcgcgcc
    33061 ggcgctggcg gcgggcaata cggtgatcct gaaaccggcg gactggtcgc cgctgtcggc
    33121 ctcggtgctg gcggatctga tcgccgaggc gggcttcccc gcgggcgtgt tcaacatcgt
    33181 ccagggcctg ggggcagagc tgggcaatgc gctcacctct gatccgcgca tcaagcgcat
    33241 cagcttcacc ggctctgtgc ccaccgcgcg ggtgatcggc cgggcggcgg ccgacaatat
    33301 cgtgcccttc accgccgagt tgggcggcaa atcgccgctc ctcgtctttg ccgatgccga
    33361 tatcgatgcc gccgccaaga aggccgccgg ccagtatgac gacagcggcc aggtgtgcat
    33421 ggccggcacg cggatcatcg tggaagacag cgtgaaggag gccttcctcg aacgcttcca
    33481 ccactatgcc gaccagcatg tgatgggatc gagcctggat ccggccacca ccatgtcggc
    33541 gctgatccat ccggtgcatg ccgagcgggt gctgggcttc atcgaccgcg cgcgcgccga
    33601 tggcgacacc atcgcccggg gcgggcgccg ctggaaggaa gggcagaact ggatcgagcc
    33661 gacgctgatc ctgcccaaaa gcaaccagtc ggaatgcgtg cagagcgaga tcttcggccc
    33721 ggtcctcacc ttccagacct ttgtgggcga ggaagagggc gtggcgctgg ccaattccac
    33781 cgcctatggc ctctcctgca tctgctacac cggcagcgag gaacgcgcgg gccgcgtggg
    33841 gcgccagttg cggggcggca cggtgtgggt caacaccttc ctcgtgcgcg atctcaccgc
    33901 cccctttggc ggcatcggca tttccggcat cggccgcgaa gggggcgatt atgccctgga
    33961 tttccattcc gacctgaaga cgctgcagat cctcgaaggc tctgtcgcct gatctcgcgg
    34021 ggcggcaggg cacgcctgcc tgccgccccg ccatgtggtg ccctgaggtt accacccttc
    34081 cccccgaagt cagctgatct ttgcctgcgg aagctgccaa cactggcatc gcggcctgcg
    34141 ccccgccgcg ccttgagggg ccgccagaac aaggatgtcg agcatgtccg ccaccatcac
    34201 cgctcctgct gccctcaccc ctgcccccgc cccggctgct gcgcccgccc acgccggcgg
    34261 tttcctcgcc agcgtcgatg ccatgtatgc ccgcgccgcc cagctggtgc cgatggaaga
    34321 ggggctttcg gaaaagatcc gcatctgcaa cgaaacctat atcacgcgct ttggcgtgcg
    34381 gctgcgcggg cggatgtaca ctttcgaggg ctggcgctgc gtgcacacca accacccggc
    34441 gccggccaag ggcggcatcc gctatgcccc cgatgtcgat caggacgagg tggaggcgct
    34501 ggccgcgctg atgagctata aatgcgcgct gatgggcctg ccctttggcg gctccaaggg
    34561 cgcgctgaag atcgaccccg cccagtggac cccttccgag ctggagcgca tcacccgccg
    34621 cttcgcccag gagctgatcc gccagaactt cctctccagc tcgctcaacg tgcccgcgcc
    34681 tgatgtcggc acatcggaac gcgaaatggt gtggattgcc gacgaatacc gccgcatgcg
    34741 cccgcatgac atcaacgccg atgcctgcgt gaccggcaag ccgctggccg ccggcggcat
    34801 cgagggccgg cccgaggcaa ccgggcgcgg cgtgcaatat gccatccgcg cgctgttcaa
    34861 cacgccggcc gatgtggcgc gcgcggggct gccggcggat ctttgcgggc ggcaggtgat
    34921 cgtgcagggg tttggcaatg tcggctatca tgccgcgcgg tttcttgccg aggatggctg
    34981 cctcatcacc ggcattgccg agcgcaatgg cggcatcgtc aacccgcagg ggctggatat
    35041 tgccgcgctg cacacccact ggcgcacgca tggcacgttc ctcggctttg ccgggggcga
    35101 ttttgtgccc cgcggcgagg atctgctgga gatggcctgc gatatcctca tccccgccgc
    35161 gcgcgaatcg gtgatcaccg gcgccaatgc cgcgcgcatc caggcgccgc tgatcgtgga
    35221 ggcggccaat ggcccggtca gccacgaggc cgatgcgcgg ctgcgggcgg ccggcaaggt
    35281 gatcatcccc gatctcttcg ccaatgccgg cggcgttacg gtgagctatt tcgaatgggt
    35341 gaagaacatc gcccatctgc ccttcggcct gatggagcgc cgccagcgcc agcgcgacac
    35401 ctcgcagctg accagcgcgc tggagcagtt gctgggcgtg gcgatgcccg acgagctggc
    35461 ccagattggc gctgcgcgcg agatcgatct ggtgcgatcg gggctggagg agaagatgcg
    35521 cgccacctat gcgcagatgt cgcatctgtg gaacagcgcc gagcagatcg acgatctgcg
    35581 caccgcggcc tatgtcatcg ccctcacccg catccgcggc ctttacgagg cgatcggcat
    35641 ctgagccagc gggcgggttg cctgacagct atcattttat gaagttgtca gcggcccgcc
    35701 cgcctctacc ctgccgcccc gacacaggca ggagcgggca ggcgatgaac ggggcaacag
    35761 gcgaatcggc acggctagag gccaaccggc aactggtgcg cgcctatctc gatgccgtca
    35821 gcttctggaa gctggatgtg atcaccgccc tttccaccga ggatgtggtg tttgaagtgc
    35881 ccttctgccc gcccggtttc gcccgcgtca cgcaggggcg cacccgctat gtggaagtgc
    35941 tgcgccaggc cagcacgctg atgatcgacg ggccggaaaa cctccacgcg atcaccgtcg
    36001 attgcctagc cggccagccc gacgagctgg tcgccgccta taaaagcacc atggtgatgc
    36061 gctctggcgc ggcctatgcc aatgaatatc tggcccgttt ttccctgcgc gatgggcagg
    36121 tggcgcgctt tgtcgaatat tgcgatccca tccgcctgta tgtggcgatg ggcggccagg
    36181 tgccgccgct gcaggcgctg gccgatagct cgatgctgcc cgatctgctg cgcggctgaa
    36241 gggcctgccc cctcacgtag cttcccttat cctggcaaca tcaatttcgt gaaagccgcg
    36301 cgttaggctg gcgccatccc tctggcatca agcggttggc ttttcccatg gcgctcttcg
    36361 gcaatctcag gatcaagacc atctctctgg cgggcaccac ggtgctggcg ggcgttgcca
    36421 ttgtctgcgc ggcgtgcgtg ctggtcgtct cgcggcagat cggcgacaat ctggcctatg
    36481 ccgagaacaa caccatcccc agcatcgtcg cgctcaacgg catcggcacc ggcctgaccg
    36541 ccgtcaggct cgatctgggc catgccgtgg ccgcgcgcga tggcgccacc cggcaagccg
    36601 cgctggccaa atatgccgcc gacaccgcca ccgtcgacag ccgcgtcaag gcctacaccc
    36661 ccttcatctc cgacgatgcc gaaaggcgcg tgttcgacaa tctcaccacc cgctgggggc
    36721 aatatcacga tctggccgaa cagctggcgc aaatggccaa ccgcgatccc gaagccgcct
    36781 atcgcctgtg ggaaggcccg ctgggccagc tgggcccgca gctgttcacc gcgctggaag
    36841 ccgatgtggc ctataatcag ggcattggcg cgagggcagc ggcggcgggc aatgccgcga
    36901 tggcccgcgc caatctgctg gcctgggtgc tgatcatcgt ggcgacagcg gtgggggcgg
    36961 gcattgtcag cctgctgctg ctgcgggtga tcggcccgct gggccggttg accggggcga
    37021 tgaacgagat ggccgccggc cacctcgacc ggcaggtgcc cgggctggaa cagcatgacg
    37081 agatcggcga tatcggccgc gcgctgctgg ccatcaagca aagcgtggcc gcccgcgccg
    37141 aagccgaagc ccatgcccgc gaggccgtgc agcagcaggt cgtctcggcg ctgggcaacg
    37201 ggctggcggc gctgaaggcg ggccagctgg cgatcagcat cgatgcgcgc ttccccgagg
    37261 aatatgtcca gctgcgcggc gatttcaacg cggcggtggc caccatggcc gatctgatgc
    37321 ggcaggtggc gggcagcgcc ggctctgtcc acagcggcgc cagcgagatt tccgccgcgg
    37381 ccgccgatct tgccaaccgc acgcaggggc aggccgccaa tctggaggaa tcggccgcgg
    37441 ccgtgcgcga actcacgcaa tccgtcacca gcgcggccgc caccgcttcg gaggccgcca
    37501 cccttgcccg caccgcccag ggcaatgccg ccaccagcgg cgaggtgatg gcccgcgccg
    37561 tgcaggcgat ggaccagatc gcccaatcca gccggcggat ggaagagatc gtcgcgctga
    37621 tcgagggcat cgctttccag accaacctct tggcgctgaa cgccggcgtg gaagccgccc
    37681 gcgcgggcga tgccggcaag ggctttgcgg tggtggccac cgaagtgcgc gcgctggccc
    37741 agcgcagctc ggatgcggcc aaggatatca ccggcatcat ccgctccagc ggccatgatg
    37801 tggccaccgg cgtggagatg atcagccaga cgcaatcgac gctggggcag atcgtctcgg
    37861 gcaccgagga gctggcgatc atgatcgacc atctggccga ggccgcgcgc gaacaatccg
    37921 ccgcgatcat gcaggtggac agcgtggtgg gcgagatgga ccgcgccacc cagcagaatg
    37981 ccgcgctggt ggaggaatcc accgccgccg cccgcagcct ggccagcgag gccaatgtgc
    38041 tgagcggcct ggtctcccgc ttcgatctgg gccaggccgc aggcggccac accagccgcc
    38101 gccgcgcggc ctgatcggct tagccggcgc cctcggccag ggcgcgggcc gcggccagaa
    38161 aatgcggcag ggcggggttg gcattggcct ggcgccacac cagcacctgc tccaccacgg
    38221 gcgctgccac cagcgggcgg aacaccaccc cgcgcatccc cagccgctcc agcgaggcgg
    38281 gcaccagcgc caggccgatc ccttcggcca ccaggctgac gatcgtctgc tgcagctggg
    38341 tttccaggcg gatcagcggc tctgccccgg cgctggcgaa ataggcggcg atcgcctggc
    38401 gcaggctggg ggtgacatcg gcc
//
LOCUS       DASRYE010000318         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_264660,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000318 DASRYE010000000
VERSION     DASRYE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264660"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFF98_16480"
     CDS             <1..334
                     /locus_tag="VFF98_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65286.1"
                     /translation="LSFEGAAITGRLSTATAAPATGTAPTRATYREIGHVVNTAQPVP
                     GGKGLSVALDGTSQWTVTGTSYLTELKLAPGAKLLAAQGKLALSVNGRKVAVRPGTYS
                     GAITVALD"
     gene            complement(486..1271)
                     /locus_tag="VFF98_16485"
     CDS             complement(486..1271)
                     /locus_tag="VFF98_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZU65287.1"
                     /translation="MTTGEEKPRGPRAVSRVLELFLLLSRHRQGLGLAQISAELGVAK
                     PTLLETLRGLCELNYLLVDVGRYRLGANAYRMASKILSAWSPPETIRREVLALAEQTH
                     ESVGFAIADWEIGQAIYTEAINSTQAVRYAMHSGIRAPLYASAAGRVLLAFADPARVD
                     AYFARASLRPLTSATKTSHADIRANLGDIRAQGYCASFGEMLSDTAAIAVPVFAPDGQ
                     VIGALMLAAPLDRMRRNTDHFLAATIEAGHRASGGGPDGPGEA"
     gene            1414..2253
                     /locus_tag="VFF98_16490"
     CDS             1414..2253
                     /locus_tag="VFF98_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZU65288.1"
                     /translation="MATSDSRTIKSAHRVLEILEYFDQNRTHATVMDMARSLSYPQSS
                     TSELLRCLTRLGYLHYNRFRRTYSPTARVALLGSWVRPSLFRGGALLHAIDSLCEKAG
                     QTVVLSSACNYVVQHLHVVQGAEAGALPVHVGDAVALLHSAQGKLLLSSYTNEQVRSV
                     VHRLNAEAGDQAHHVKIADFLDELEQMRKGGWAVAHGADGHSAIAAILPPLRGMDRMA
                     ISLVATTDQLLQRESELLAAVLHTRDEVAKGCAEAMPCAVPANVVPMKQEMKILSYRR
                     HYA"
     gene            2399..3565
                     /locus_tag="VFF98_16495"
     CDS             2399..3565
                     /locus_tag="VFF98_16495"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZU65289.1"
                     /translation="MGHSLLARLDHYLDAAATACGHTDFAEDFAGASLREGLARFLAD
                     LDAEDRLSAQGLAATEAQIGLMLRSRLETVAAWKAHPAYRAVDIRRPLIITGIVRSGT
                     TALHRLLTVDPQFLGVPHWLVRSPRPLPPRAGWAADPQFQAASAVLERMIAQAPEMLN
                     DHMMTAEAVEESIFLLAPTFCNNMYPSQWTVPAYDAWYRAADEVPAYRWLADCLRLIG
                     LEAPGRPWLMKNPTDLHAIDALFTVFPDARIIHTHRDPVQAIPSITHLLLSVRRMFEG
                     ARADPAQVLRREGAFWAQALARAESARQQQPDRFVDISFAAFVKDQMAAVEQIYAQLE
                     LELAGPVADAMRAWLAAHPRRPGHIQRHRAEDFGVATGWLEEIFAPYRQRHGYG"
     gene            3576..4703
                     /locus_tag="VFF98_16500"
     CDS             3576..4703
                     /locus_tag="VFF98_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65290.1"
                     /translation="MAEATDIAERALALVALVHEGVFEPTPWQRFLRALRGQAGAGYA
                     NVIFRHPGAPDDGLTEWVDADDDPQAIRDHYFASFAGRDPFPYFRMEPGRIMRMPELM
                     GDPDFRRKPYFTDFLKPQGLEHMLLFRAVEPGGHQAWVTLTRPLDGQDFAEETARLCA
                     RIVPHFAIALRAYGELEAARITRGVRDRALRPAGLACVTVDLRRIVVHGDTDRLGTAA
                     DRPVHLDGGRRLRLAHAEADRRFGRALADIDRGHLTAPQTMPCGSDADEQILLEPLPC
                     HLRRTHGAIANVYFRDAGADTPDTAAFRGALRRIYGLSANEATLACLLAGGATLADAA
                     RQIGLTEESARTYSKRIFAKTGTSRQPALVSLLLRSIATLA"
     gene            4846..>5019
                     /locus_tag="VFF98_16505"
     CDS             4846..>5019
                     /locus_tag="VFF98_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HZU65291.1"
                     /translation="MNAIAARPTEHLTHLARLEAESIHIIREVVAEADRPVMLYSVGK
                     DSAVMLHLARKAFY"
BASE COUNT          791 a         1799 c         1632 g          797 t
ORIGIN      
        1 cctctccttc gagggcgcag cgatcaccgg ccgcctctcc accgccaccg cagcgccggc
       61 caccggcacc gcccccaccc gcgccaccta tcgggaaatc ggccatgtgg tgaacaccgc
      121 ccagccggtg ccgggcggca agggcctcag cgtggcgctg gatggcacct cgcaatggac
      181 ggtgaccggc acttcctacc tcaccgagct gaagctggcg ccgggcgcaa agctgctggc
      241 cgcgcaaggc aagctggcgc tcagcgtcaa cggcaggaag gtggcggtgc gcccgggcac
      301 ctatagcggc gcgatcaccg tcgcgctcga ctgaacacgg gcggcgcccg gccagggccg
      361 ccgcccaccc cacaaccggc cgcgcgcgcg agatgatcgc aaccgcggcc gatccggtcc
      421 tgcacctccc cgcatggccg acagactggc gcagctgccc gggcccggca gctgcgccat
      481 ttttctcaag cctccccggg cccatccggc ccgccgccag aggcgcggtg gccagcctcg
      541 atggtggcgg caaggaaatg gtctgtgttg cggcgcatcc ggtccagcgg ggcggccagc
      601 atcagcgcgc cgatcacctg cccatccggc gcgaacaccg gcacggcgat cgcggccgtg
      661 tcgctcagca tctcgccaaa gctggcgcaa tagccctggg cgcggatatc gcccagattg
      721 gcgcggatat cggcatggct ggtcttggtg gcgctggtca gcgggcgcag cgaggcgcgg
      781 gcgaaatagg catccacccg cgcaggatcg gcaaaggcca gcagcacacg gcccgccgcg
      841 ctggcataaa gcggcgcccg gatgcccgaa tgcatggcat aacgcaccgc ctgggtgctg
      901 ttgatcgcct ctgtgtagat ggcctggccg atttcccagt cggcaatggc aaagcccacc
      961 gattcatgcg tctgctcggc cagcgccagc acttcgcggc ggatggtttc cggcggcgac
     1021 caggccgaga ggatcttgct ggccatgcga taggcattgg cccccagccg atagcgcccg
     1081 acatcgacga gcaggtaatt cagctcgcac aggccgcgca gggtttccag caaggtgggc
     1141 ttggccacgc ccagctcggc gctgatctgc gccaggccaa ggccctggcg gtggcgcgag
     1201 agcagcagga acagctccag cacccggctg accgcgcgcg ggccgcgcgg cttttcctcg
     1261 cctgttgtca ttggcccggc cttccccttc tcctgctccc tgccgccctt ctagatattc
     1321 cgatttcgga atgcaattcg gaaaatattg cacccccatc ggcccgatga tatccccatg
     1381 gcaattacaa aaccgattcg agggagaatg cagatggcca ccagcgactc cagaaccatc
     1441 aaatctgcgc atcgcgtcct tgaaatcctt gaatattttg atcagaaccg cacccacgcc
     1501 acagtgatgg acatggcccg cagcctgtcc tacccgcaat cctccacctc cgagctgctg
     1561 cgctgcctca cccggctggg ctatctgcac tacaaccgct tccgccgcac ctacagcccc
     1621 accgcccgcg tggcgctgct gggcagctgg gtgcgcccgt cgctgttccg tggcggggcg
     1681 ctgctgcatg cgatcgattc gctgtgcgag aaggccgggc agacggtggt cctctcctcg
     1741 gcctgcaact atgtggtgca gcatctgcat gtggtgcagg gcgcagaggc gggcgcgctg
     1801 ccggtgcatg tcggcgatgc ggtggcgctg ctgcattcgg cgcagggcaa gctgctgctc
     1861 tccagctata ccaacgagca ggtgcgctcg gtagtgcacc ggctgaatgc cgaggcgggc
     1921 gatcaggccc accatgtgaa gatcgccgat ttcctcgacg agctggagca gatgcgcaag
     1981 ggcggctggg ccgtggccca tggcgccgac gggcacagcg ccatcgccgc catcctgccc
     2041 cccttgcgcg gcatggatcg catggcgatc agcctggtgg ccaccaccga ccagctgctg
     2101 cagcgcgaga gcgagctgct ggccgccgtg ctccacacgc gtgacgaggt tgccaagggc
     2161 tgcgccgagg cgatgccctg cgcggtgccg gccaatgtgg tgccgatgaa gcaggagatg
     2221 aagatcctca gctatcgccg ccattatgcc tgacggcggg cgggcgctgt tcccgcatgc
     2281 agcgccccgg cgctgttcct gcatgcagcg ccctggcgct gttcccaaac gggaatcccg
     2341 ccccgccagc caccgcacta cccctcggcc tttcacacaa ccatgtcggg aggataactt
     2401 ggggcattcc ctgcttgcgc ggctcgatca ctatctggat gcggcggcca ccgcctgtgg
     2461 ccacaccgat ttcgccgagg attttgccgg cgccagcctg cgcgaggggc tggcccgctt
     2521 cctggccgat ctcgacgccg aggaccggct gagcgcgcaa ggcctggccg ccaccgaggc
     2581 gcagatcggc ctgatgctgc gcagccggct ggaaaccgtg gccgcctgga aggcccaccc
     2641 cgcctatcgc gcggtggaca tccgccggcc gctcatcatc accggcatcg tgcgttcggg
     2701 caccaccgcg ctgcaccggc tgctgacggt ggacccgcaa ttcctcggcg tgccccactg
     2761 gctggtgcgc agcccgcgcc ccttgccgcc gcgcgccggc tgggcggccg atccgcagtt
     2821 ccaggcggcc agcgccgtgc tcgaacggat gatcgcccag gcgccggaaa tgctgaacga
     2881 ccacatgatg acggccgagg cggtggagga atcgatcttc ctgctggccc ccaccttctg
     2941 caacaacatg tatccctcgc aatggaccgt gcccgcctat gatgcctggt atcgggcggc
     3001 agacgaggtg cccgcctatc gctggctggc cgattgcctg cggctgatcg ggctggaggc
     3061 gcccggccgc ccctggctga tgaagaaccc caccgacctg cacgccatcg acgcgctgtt
     3121 caccgtgttc cccgatgcgc gcatcatcca cacccaccgc gatccggtgc aggcaatccc
     3181 gtcgatcacc catctgctgc tgtcggtgcg gcgcatgttc gagggcgccc gggccgatcc
     3241 ggcccaggtg ctgcgccgcg agggcgcgtt ctgggcacag gcgctggccc gcgccgaaag
     3301 cgcccgccag cagcagcccg atcgcttcgt cgacatctcc tttgccgcct tcgtgaaaga
     3361 ccagatggcc gccgtggagc agatctatgc gcagctggag ctggaactgg ccggccccgt
     3421 ggccgatgcc atgcgcgcct ggctggccgc ccatccgcgc cggcccggcc atatccagcg
     3481 ccaccgggcc gaggattttg gcgtggccac cggctggctg gaagagatct tcgcccccta
     3541 tcgccagcgc cacggctatg gctgaggcca aggctatggc tgaggccacg gatatcgccg
     3601 agcgggcgct ggcgctggtg gcgctggtgc atgaaggggt gttcgagccc accccctggc
     3661 agcgtttcct gcgggcgctg cgcgggcagg cgggggcagg ctatgccaat gtcatcttcc
     3721 gccaccccgg cgcgcccgat gatggcctga ccgaatgggt ggatgccgac gacgaccccc
     3781 aggccatccg cgaccattat ttcgcctctt tcgccgggcg cgatccgttc ccctatttcc
     3841 gcatggagcc ggggcggatc atgcgcatgc ccgagctgat gggcgacccg gatttccgcc
     3901 gcaaacccta tttcaccgat ttcctgaagc cgcaggggct ggaacacatg ctgctgttcc
     3961 gcgcggtgga gccgggcggg catcaggcct gggtcacgct tacccgcccg ctcgacgggc
     4021 aggattttgc cgaggaaacg gcgcgattgt gcgcgcggat cgtgccgcat ttcgccattg
     4081 ccctgcgcgc ctatggcgag ctggaggccg cccgcatcac ccgcggcgtg cgcgacaggg
     4141 ccctgcgccc ggcggggctc gcctgtgtca cggtcgatct gcggcggatc gtggtgcatg
     4201 gcgataccga ccggctgggc accgccgctg atcggcccgt tcaccttgat ggcggccggc
     4261 ggctgcggct ggcccatgcc gaggccgatc gccgcttcgg cagggcgctg gccgatatcg
     4321 accggggcca cctcaccgcc ccgcaaacca tgccctgcgg cagcgatgcc gacgagcaga
     4381 tcctgctgga acccctgccc tgccatttgc gccgcaccca tggggcgatc gccaatgtct
     4441 atttccgcga tgccggcgca gacacgcccg acaccgccgc ctttcgcggc gccctgcgca
     4501 ggatctacgg gctgagcgcc aatgaggcaa cgctggcctg cctgctggcg ggcggcgcca
     4561 ccctggccga tgcggcccgc cagatcggcc tcaccgaaga atccgcccgc acctacagca
     4621 agcgcatctt cgccaagacc ggcaccagcc gccagcccgc gctggtcagc cttttgctgc
     4681 gcagcattgc cacgctggcc tgagcgcttt gttcccgaaa gggaactgcg cgcccgcctc
     4741 gccatcggca caaacagggc aagaggcagc aacagccccc tcgccatcgc ccccggccat
     4801 cggccgcggc ggccaggccc ccatccatca ggaaggctcg ggaagatgaa cgccattgcc
     4861 gcaaggccaa cagaacacct cactcatttg gcgcgtcttg aggcggagag tatacatatt
     4921 atacgggagg ttgtggcgga ggcagatcgt cctgtgatgc tgtattcggt gggcaaggat
     4981 tcggcggtga tgctgcatct ggcgcgcaag gcgttttat
//
LOCUS       DASRYE010000319        14623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_264741,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000319 DASRYE010000000
VERSION     DASRYE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264741"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFF98_16510"
     CDS             <1..550
                     /locus_tag="VFF98_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU65292.1"
                     /translation="SAAAGATGSLACQVGRNLGAKVVGIAGGPAKCRWLREELGVDVA
                     IDYRGQDVAAELAKIDGGINAYFDNVGGPLLDAVLPNMAHYGRVAVCGMVAAYDNDAP
                     LPGPARFDQVLMRRLRIEGFFIPDFLHRGAEFLPVLRQWVEEGRLAMPFDESEGLENV
                     LTAYERMLTGKSTGKVIVKVAP"
     gene            550..1353
                     /locus_tag="VFF98_16515"
     CDS             550..1353
                     /locus_tag="VFF98_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZU65293.1"
                     /translation="MPQIDYHAPLKVLVAVRGHPFDRNAFDGLFAGMSGISATMVDQP
                     AAALLINPQGMADFDALVLYDMPGLDFAASENPPASVAPPEALRQGLPALLRQGKGVV
                     ALHHALAGWPGWAEYHDWLGGQFLYRGGAAGGGQWPCSGYRHDVTHEVEVLANHPVTA
                     GLPPRFTLTDELYLCPVWAEAQGATPLLRSHAPFTTDHFWSAELAVAGRMFCRDGWQH
                     PPGSGLVGWARQVEAAKLVYLQPGDGPETYANPHYRQLVENAIRWVAQR"
     gene            complement(1415..2194)
                     /locus_tag="VFF98_16520"
     CDS             complement(1415..2194)
                     /locus_tag="VFF98_16520"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZU65294.1"
                     /translation="MGRLQGKIALVTGGASVPGLGSATAMRFAEEGAFVYVTDRDGAG
                     AEKVAASIREAGGKAGALAHDVTSEAAWDAVFAAIEADHGRLDVLVNNAGIAVLRPIA
                     EMTTADWTLQNSVNLDSVFQGTKRAVALMRRKNADGSPRGGAIINISSVAGLIGVPAC
                     GAYAAAKGGVRLFSKTIAVECAKDLIRVNSVHPGMILTNMQGVAQEDNAANYDATLAL
                     VPMGFMGDPLDIANMNLFLASDEARYVTGTELVVDGGMTAF"
     gene            complement(2278..4605)
                     /locus_tag="VFF98_16525"
     CDS             complement(2278..4605)
                     /locus_tag="VFF98_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65295.1"
                     /translation="MEGFAARPRMLALALTTALTTTCFAMPALADEAPAAAPASPVVD
                     NGAIIVQARRRSETLQTTPIAITALNPAMLESRAATNLGDLQGAAPGLLITQQNSGAA
                     AANLSIRGLTYADIEKSQTPTVGVVVDGITIGTNTGQLQDVFDVAQIEVLRGPQGTLF
                     GANTIGGVVNIQRSKPTMEPGGKVEFSYGQWNTWSGKAILNYGNGSTWGVKGWYFHNQ
                     TDGYYWNAATNKRAGGSKDDNYGGSVLFRPTGSGFDAQLTVENVVQSFDPVNSTIANS
                     SDGFCAAGYIPAASCNRNTTTDLYTVFDQPAYAHYHAPAATLNMNLDLGAVKLTSITG
                     WRHSREEQTQDFGTDGVYYVLRKQHYTQWSQELRAAGKLIRGLDYVVGGYFFDSNYDL
                     TQWTGGPALYNTSNPDAVAPGPQIVHGHTQSFALFGDFNWQIVSKVRLSFGGRWSHDH
                     KDLTDAIGAFPGCVTYTASNYDTTGNCGTFGDGHISFSKFTPKVGLDWRPNNNMMVYA
                     SWSRGYRSGGYSGRALTWDSAHTPFGPETVDAYELGTKLSTPDHRGTLNIAGFISNYK
                     NMQQNETIPGGPVGDQTITVNVGGAVIRGIEMDGSFKLTPQLRISATASLMESHFRNF
                     VVGNVYGSTLEPFDFSGNHLIYAPTFSGSLNAEYTVPTSFGKVVSTFGWRHISPYDEQ
                     ISIASTTAGTVNGETGRIVNGNDPRVRTVTQDLVDASITANFKLHGTDAYVRVFGRNL
                     ADVRTTTAAFTVAGLWSFASAIEPRTWGVTVGSKF"
     gene            4769..5782
                     /locus_tag="VFF98_16530"
     CDS             4769..5782
                     /locus_tag="VFF98_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZU65296.1"
                     /translation="MSENRRFLLMRRPAGDPAPDDFQLVTEPVPPPPPGGFVVRNQFI
                     SLDPAQRGWMDDTPSYLPPIALGDPVRATTVGRVHASDHPDFAPGDWVMGLNAMEDFS
                     QVLPGGFTQKIDIGLVERPSQFLSAMGAVSLTGYFGLIDAGQPRAGETLLVSGAAGAV
                     GSVVGQIGKILGLRVIGIAGGAAKCRRLVEDYGFDAAIDYKGKGIEELAAEIGAAAPQ
                     GADIIFENVGGDVLEAEMFNLAQGARIVLCGLISEYNSPEKHGVRNLWQVLVKQATIR
                     GFLISGYVSRFAEGGAQIAQWVADGRLRIDEDVQQGLENAYPAFMRLFSGANTGKLVL
                     QIA"
     gene            5807..6325
                     /locus_tag="VFF98_16535"
     CDS             5807..6325
                     /locus_tag="VFF98_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65297.1"
                     /translation="MAIDDTALAALLDEAVIARLIARYASMTDWLDWEAIRPLFTADA
                     HFDFGAMCRGGLEAYLPFVQPMEAGYDRRMHMMGLPRVELAGDEARAEAPSLIHARNH
                     ASDGSDHHADLLFYGRYMFTARREGGTWKLASLRYYLSALYPLAAEVMDESALNTATG
                     FAPSHADAMRHG"
     gene            complement(6395..7579)
                     /locus_tag="VFF98_16540"
     CDS             complement(6395..7579)
                     /locus_tag="VFF98_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZU65298.1"
                     /translation="MTKIYRKYGEIAERMLHFVETKTTDQASATMQVPVENYLDEGRW
                     HKEIDRIFKRLPLMLALTIELPNPGDYKAMQVMGLPVLITRTRDGKAHAFLNVCKHRA
                     MLMVSEGKGHCSRFACQYHGWTYANDGQLIGIAEAGTFGEVDKSRLHLTELPCDEAAG
                     LIFVILTPGLPIDAVAWLGGMYEDFAALKLDTWYYHKSREMKGANWKVAYDGYLEGYH
                     FQAAHTNTVATRSPSNRAHYEGFGPHIRLGFPQNAITRLHDLPREEWGLQENKGYDFI
                     RMLFPNMSFFLAPEMGQLAQLFPGPEVNQNTTVMNYIFPTKPETEEGLKKLDEMSDFF
                     FDVVEEEDYFLGLKVQTGLESGAMTHQTFGRNEPGNQFFHKWVAYYLDETGQTPVPVM
                     KG"
     gene            complement(7585..8997)
                     /locus_tag="VFF98_16545"
     CDS             complement(7585..8997)
                     /locus_tag="VFF98_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU65299.1"
                     /translation="MADFDASEGFRPARCPGKSWEDILREDDVAPPAFVAEDRYRYLG
                     SEAIDAARYYDPAFFRAEQEKMWPNVWQFAAREEDLPEPGDYVTYENAGRSFLLVRQD
                     DDSIRAFHNVCLHRGRKLKTDSGSADQFVCPFHGFAWNTDGSLRGIPCRWDFGHLSDE
                     KLQLPEASVARWGGYVFLRENPEGPSIEEFLAPLPEFFKRWKHEDCVTVAWVGKVVKA
                     NWKITMEAFMESYHAFVTHPQLMPFTGDANAAYHVLGRHVNVNYTPFGVISPHLEQGL
                     PEQWIVDEFVKYNGRSADNYDPEKDHYAIAVPEGASARQALGARMREVSARQFGGDYA
                     DVSESELLDALVYNVFPNFAPWGGFMPNIVYRWRPWPDQDHCLMEVRVVARIPEDQRG
                     GKRPHGVPFHLLAEHETWADAPELGVLGGVVDQDMTNMELTHEGLKVSKNQKVELGDY
                     QEVRIRHFHQTLDSYLNAKG"
     gene            complement(9181..9675)
                     /locus_tag="VFF98_16550"
     CDS             complement(9181..9675)
                     /locus_tag="VFF98_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU65300.1"
                     /translation="MAIGVEGVHHIGISVPDLAKARAFYIDLLGGVEEVAPLAWRDNP
                     AIDAVVGLKGSAADQFMVRLGNTHIEVFEYHAPRSAPQEPDRGVHNFGYTHFGVQVSD
                     LKACYERLLAAGIRVHAEPAWDSITTHPDGSKTGYAATYCRDPFGNVFEIMEIHETDE
                     IKRV"
     gene            complement(9778..10539)
                     /locus_tag="VFF98_16555"
     CDS             complement(9778..10539)
                     /locus_tag="VFF98_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65301.1"
                     /translation="MDLGLKGKKVILTGGSRGIGRATAELFAAEGADVAFCSRNAEQV
                     AETAAALASHGGKVVGEAFDMEAGQDAYRAWLKGAAEKLGGCDIFVPMISTSGAGATG
                     DWHKSLDYDIMGSVIGVEVLEEQLAASGTGSIVLMGSTAGLETFIVPQGYNALKGALI
                     VYAGQLSQALGGKGIRVNTVSPGPIKFAGGNWETIKELAPGLYDATEAQFALGRWGGP
                     EEVAKTIVFLASPASSYTTGTNVVVDGGFTKRVQF"
     gene            10708..11514
                     /locus_tag="VFF98_16560"
     CDS             10708..11514
                     /locus_tag="VFF98_16560"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65302.1"
                     /translation="MGRLAGKRAVVLGASSSDNMGQHIARRFLAEGAEVLVSGRKGEV
                     LEAFAGETGCQWHPCDLTAPASVAALADAAAQKLGGIDIAVNATGWGLLKGFLETTHE
                     DLLEMTALQFIGPYHFYQAMIGKMAFSKGGRGGVLLQISSATAKIMLNDHAAYMGTKA
                     GTDHVIRCVAHEFGAEGIRANSISPGLTDTPMTAHAKTVPGLFESFLAGYPLGRIGTS
                     DDIAAAAVFLASDECFMTGENLQVNGGLTLRRNPTREEMAAVISAAHNKG"
     gene            11523..12995
                     /locus_tag="VFF98_16565"
     CDS             11523..12995
                     /locus_tag="VFF98_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65303.1"
                     /translation="MTTDIPAILRAADIARWDREVDVVVIGQGAAGTCAALEAHRAGA
                     EVLIVERASGGGGASAMSSGIYYLGGGTAVQKACGYDDDAEQMAAFMMASTDPTDPMA
                     VHAFCADSAAHFDWLEAQGVPFERSAFKGKAVFLNTTECLMYTGNEKLWPYSEVARPA
                     PRGHHVAGTGENAGAVGMAPLLERCAQEEIPALYDARVTALVQDAGGRVVGVSVKQSG
                     GMLHVAARKGVIIAAGSFNLNREMIAHNIPLIAETSEPLGISSNDGAGVLLGLSAGAA
                     TSAMDGIIPTASIYPPAQLIKGIIVNARGERFVAEDSYHGRTANFIMEQPGQKAYLIL
                     DSEIFAYPEITSARHRLIDGWETVAEMEAGIGLPEGSLATTLARYNADAAAGKDSLFH
                     KAADWLKPLDQGPYAAFDISFDTSIYLFITLGGLKTDVEGRCLDLYSQPVPGLYAVGA
                     SAAHIPRTGKAYASGMSLGPGSYFGRRAGRHAAGAPWPQG"
     gene            complement(13117..>14623)
                     /locus_tag="VFF98_16570"
     CDS             complement(13117..>14623)
                     /locus_tag="VFF98_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU65304.1"
                     /translation="ADPGFIAFVEGEAQLSRAELVGDAEALAVALHERGLRPGDVVAF
                     QVPNWREAAVINLAAALSGLIVNPIVPIYRDHEVRLMLADCRARAIFVAASFRNYDFG
                     AMAARIRADLPDLAHVFTVRGSGPDDYAALLAEGRGKAFARPAVDPMGVKMVLYTSGT
                     TGRPKGVLHSHTTLQHVLRKSHAHWGVAPGEATLMPSPVTHVSGYANGLEAPLLCGIR
                     SVLMEAWKADEALALIERHQLVGTVAATPFLVELAAAARAAGTQLPSFRFFACGGAAV
                     PPELIPAARAAFAHCRPFRVFGASEVPLVTLGWPHAGEEDLAATTDGEVVDYEVRIVD
                     AEEQPLPAGQEGEILARGPAMMMGYADEGQTAEAITLDGFFRTGDLGVLDARGALTIT
                     GRKKDLIIRGGENISAKEIEDVLHTHPLVKEASVVAMPHDRLGEGVCAYVIATGAVEA
                     GQLATHVAASGLAKQKIPERFEFVSDFPRTASGKIRKDQLRADIKAKLEAA"
BASE COUNT         2467 a         4887 c         4746 g         2523 t
ORIGIN      
        1 gtcggccgcg gccggcgcca cgggcagcct ggcctgccag gtggggcgca atctgggcgc
       61 caaggtggtg ggcattgccg gtggcccggc caaatgccgc tggctgcgcg aggagctggg
      121 cgttgatgtg gcgatagatt atcgcgggca ggatgtggcg gcagagctgg cgaagatcga
      181 cggcggcatc aacgcctatt ttgacaatgt cggcgggccg ctgctggatg cggtgctgcc
      241 caacatggcc cattatggcc gggtggcggt gtgcggcatg gtcgccgctt acgacaatga
      301 cgcccccctg cccggccctg cccgcttcga ccaggtgctg atgcggcgcc tgcgcatcga
      361 gggcttcttc atccccgatt tcctccatcg cggcgccgaa ttcctgcccg tgctgcgcca
      421 gtgggtggag gaagggcgcc tggccatgcc cttcgacgag agcgaggggc tggaaaacgt
      481 gctgaccgcc tacgagcgca tgctgaccgg caagagcacc ggcaaggtga tcgtgaaagt
      541 ggcgccctga tgccacagat agactatcac gcgccgctga aggtgctggt cgccgtgcgc
      601 ggccacccgt tcgaccgcaa cgccttcgat ggcctgtttg ccggcatgtc cggcatcagc
      661 gccaccatgg tggaccagcc cgccgccgcc ctgctgataa acccgcaagg catggccgat
      721 ttcgacgcgc tggtgctcta tgacatgccc gggctggatt ttgccgccag cgaaaacccg
      781 cccgcctctg tcgcgccgcc cgaggccctg cgccaggggc tgcccgccct gctgcgccag
      841 ggcaaggggg tggtggcgct gcatcatgcg ctggcgggct ggccgggctg ggcggaatat
      901 cacgactggc tgggcggcca gttcctctat cgcgggggcg ctgccggcgg cggccagtgg
      961 ccctgcagcg gctaccgcca cgatgtgacg catgaggtgg aagtgctggc aaaccatccg
     1021 gtcaccgccg gcctgccgcc ccgcttcacg ctgaccgacg agctttatct ctgcccggtc
     1081 tgggccgagg cgcagggggc aacgccgctg ctgcgctcgc acgcgccctt caccacggat
     1141 cacttctggt cggccgagct ggcagtggcg ggccgcatgt tctgccgcga tggctggcag
     1201 cacccgcccg gcagcggcct tgtcggctgg gcgcggcagg tggaggcggc gaagctcgtc
     1261 tatctccagc ccggcgacgg gcccgaaacc tatgccaacc cgcattaccg ccagctggtg
     1321 gaaaacgcca tccgctgggt ggcccagcgc tgaagcctcg ggcgctgaaa cctcgcgcgc
     1381 tgccctgtcg ccaggcagcg cgcgaggcgg cgggtcagaa ggcggtcatg ccgccatcca
     1441 ccaccagttc ggtgccggtc acatagcgcg cctcgtcgct ggcgaggaac aggttcatat
     1501 tggcgatatc cagcggatcg cccatgaagc ccatcggcac cagcgccagc gtggcatcgt
     1561 aattggccgc gttatcttcc tgcgccacgc cctgcatgtt ggtgaggatc atgcccgggt
     1621 gcacggaatt gacgcggatg agatccttgg cgcattccac cgcgatggtc ttggaaaaca
     1681 gccgcacgcc gcccttggcc gccgcatagg ccccgcaggc cggcacgccg atcagccccg
     1741 ccaccgagga aatgttgatg atggcgccgc cacgcgggct gccatcggcg ttcttgcggc
     1801 gcatcagcgc cacggcccgc ttggtgccct gaaacacgct gtcgaggttg acgctgttct
     1861 gcagcgtcca gtcggcggtg gtcatctcgg caatcgggcg cagcacggcg atgccggcat
     1921 tgttcaccag cacatccagc cggccatgat cggcctcgat ggcggcgaaa acggcatccc
     1981 aggccgcctc gctggtcaca tcatgggcca gcgcgccggc cttgccgccg gcctcgcgga
     2041 tgctggccgc cactttctcg gcgccggcgc catcgcggtc ggtcacatag acgaaagccc
     2101 cctcctcggc aaagcgcatg gctgtggcgc tgcccagccc cggcaccgag gccccgccgg
     2161 tgaccaacgc gatttttccc tgaagacggc ccattcttgc atccttcgtt tcaaaaagga
     2221 ggggcgcggc ccttgcatat ggccgcgccc cttccggtgg tggtggaaat cagacgatca
     2281 gaacttgctg cccaccgtca cgccccaggt gcgcggttca atcgccgagg cgaaggacca
     2341 caggcccgcc acggtgaagg ccgcggtggt ggtgcgcaca tcggccaggt tgcggccaaa
     2401 cacgcgcaca taggcatcgg tgccatgcag cttgaagttg gccgtgatgc tggcatcgac
     2461 gagatcctgc gtaacggtgc gcacgcgcgg atcattgccg ttgacgatgc ggccggtttc
     2521 gccgttcacc gtgcccgccg tggtcgaggc gatcgagatc tgctcgtcat aggggctgat
     2581 atggcgccag ccgaaggtgc tcaccacctt gccgaagctg gtcggcaccg tatattcggc
     2641 gttgagcgag cccgagaagg tcggcgcata gatcaggtgg ttgcccgaga agtcgaacgg
     2701 ctccagcgtg ctgccataga cgttgccgac gacgaaattg cggaagtgcg attccatcag
     2761 cgaggccgtg gcgctgatgc gcagttgcgg cgtcagcttg aacgagccat ccatttcgat
     2821 gccgcggatc accgcgccac ccacgttgac ggtgatcgtc tggtcgccca ccgggccgcc
     2881 agggatcgtc tcgttctgct gcatgttctt gtagttcgag atgaagccgg caatgttcaa
     2941 cgtgccgcga tggtccggcg tcgacagctt ggtgcccagt tcgtaggcat ccaccgtttc
     3001 cggcccgaac ggcgtgtggg ccgaatccca cgtcagcgcg cggccgctgt agccacccga
     3061 gcgatacccg cgcgaccagc tggcatagac catcatgttg ttgttcggcc gccagtccag
     3121 ccccaccttg ggagtgaact tcgagaagct gatatggcca tcgccgaagg tgccgcaatt
     3181 gccggtggtg tcgtaattgc tggcggtata ggtcacgcag cccgggaaag cgccgatggc
     3241 gtcggtcagg tccttgtggt catgcgacca gcgaccgccg aacgacaggc gcaccttcga
     3301 gacgatctgc cagttgaaat cgccgaagag ggcgaaggac tgggtgtggc catgcacgat
     3361 ctgcgggccg ggcgccaccg catcgggatt gctggtgtta tacagcgccg ggccgccggt
     3421 ccactgggtc aggtcgtagt tcgaatcgaa gaaatagccg cccaccacat agtccagccc
     3481 gcggatcagc ttgccggcgg cgcgcagttc ctggctccac tgcgtgtaat gctgcttgcg
     3541 cagcacgtaa tagacgccat ccgtgccgaa atcctgcgtc tgttcttcgc ggctgtggcg
     3601 ccagccggtg atcgaggtca gcttcaccgc gcccagatcg aggttcatgt tcagcgtggc
     3661 ggccggcgca tgataatgcg cataggccgg ctgatcgaac acggtgtaga gatcggtggt
     3721 ggtgttgcgg ttacaggaag ccgccgggat atagccggcc gcgcagaagc catcgctgct
     3781 gttggcgatg gtgctgttca ccggatcgaa cgactgcacg acattttcca ccgtcagctg
     3841 cgcatcaaag ccagagccgg tcgggcggaa caggacagag ccgccgtaat tgtcgtcctt
     3901 gctgccgccg gcgcgcttgt tggtggccgc gttccagtag tagccgtcgg tctgattgtg
     3961 gaaataccag cccttcacgc cccaggtgct gccattgcca tagttgagga tggccttgcc
     4021 gctccaggtg ttccactggc cgtaggagaa ttccaccttg ccgcccggct ccatcgtcgg
     4081 cttggagcgc tggatgttga ccacgccgcc gatcgtgttg gcgccaaaca gcgtgccctg
     4141 cggcccgcgc agcacctcga tctgggccac gtcgaacaca tcctgcaact ggccggtgtt
     4201 ggtgccgatg gtgatgccat caaccaccac gcccacggtc ggcgtctggc tcttttcgat
     4261 atcggcatag gtgaggccgc ggatcgacag gttggccgct gccgcgcccg aattctgctg
     4321 ggtgatcagc aggcccggcg ctgcgccctg cagatcgccc agattggtcg ccgcgcggct
     4381 ttccagcatg gccgggttca gcgcggtgat ggcgatcggc gtcgtctgca gcgtttccga
     4441 acggcggcgg gcctgcacga tgatcgcccc attatcgacc accggactgg caggcgcggc
     4501 cgccggcgcc tcgtcggcca gggccggcat ggcaaagcag gtggtggtga gcgccgtggt
     4561 cagggccagc gcaagcatgc gcggccgggc agcaaagcct tccataaaat cctcccatca
     4621 aatcccggca actttccctt atccattggg aaagcttgct tgtgggccag attacgaggc
     4681 tttttgcgca accctactag aacaagtcac aggtgcaccc cgccccccgc gcggccagca
     4741 tccgggcaaa cgtgatgggt gagaaaatat gtctgaaaat cgccgcttcc tgctgatgcg
     4801 ccgccccgca ggcgatcccg cgccagacga tttccagctg gtgacagagc ccgtgccgcc
     4861 gccaccgccg ggcggttttg tcgtgcgtaa ccagttcatt tccttggatc ctgcccagcg
     4921 cggctggatg gacgacacgc ccagctacct gccgccgatc gcgctgggcg atccggtgcg
     4981 ggccactacc gttggccgcg tgcatgcctc cgaccacccc gattttgccc ccggcgactg
     5041 ggtgatgggc ctcaatgcca tggaagattt ctcgcaagtg ttgcccggag gcttcaccca
     5101 gaagatcgac atcggcctgg tggaacgccc ctcgcagttt ctctcggcaa tgggcgcggt
     5161 cagcctcacc ggctatttcg gcctgatcga tgccggccag ccccgggcgg gcgaaacgct
     5221 gctggtctcg ggcgcggcgg gcgcggtggg cagtgtcgtc gggcagatcg gcaagatcct
     5281 cggcctgcgg gtgatcggca ttgccggcgg cgctgccaaa tgccgccggc tggtggagga
     5341 ttatggcttc gacgcggcga tcgactacaa aggcaagggg atcgaggaac tggccgccga
     5401 gatcggcgcg gccgccccgc aaggcgccga catcatcttc gagaatgtcg gcggcgatgt
     5461 gctggaagcc gagatgttca acctggcgca gggcgcgcgc atcgtgctgt gcggcctcat
     5521 cagcgaatat aacagcccgg aaaagcacgg cgtgcgcaac ctgtggcagg tgctggtgaa
     5581 gcaggcgacg atccgcggct tcctcatctc gggctatgtc tcgcgctttg ccgagggcgg
     5641 cgcgcagatc gcccaatggg tggccgatgg ccgcctgcgc atcgacgagg acgtgcagca
     5701 gggcctggaa aacgcctatc cggctttcat gcgcctgttt tccggcgcca acaccggcaa
     5761 gctggtgctg cagatcgcct gatccgcccc gcacaggaga gaccccatgg ccatcgatga
     5821 caccgccctt gccgccctgc tggacgaggc ggtcattgcc cgcctcatcg cccgctatgc
     5881 cagcatgacc gactggctgg actgggaggc gatccgcccg ctgttcaccg ccgatgccca
     5941 tttcgatttc ggcgccatgt gccggggcgg gctggaggcc tatctgccct tcgtgcagcc
     6001 gatggaagcc ggatacgacc ggcgcatgca catgatgggc ctgccccgcg tggaactggc
     6061 cggcgatgaa gcccgcgccg aggcgccctc gctgatccac gcccgcaacc atgcttccga
     6121 tggcagcgac caccatgccg acctgctgtt ctatggccgc tacatgttca ccgcccggcg
     6181 cgagggcggc acctggaaac tggccagcct gcgctattat ctctcggcgc tctatccgct
     6241 ggcggcagaa gtgatggacg aaagcgcgct caacaccgcc accggctttg ccccctcgca
     6301 cgccgatgcg atgaggcacg gataaccggc ttcatccaac gaaaaggggg gccgcaaagc
     6361 cccccttccc tccccaagaa cccgaaaaag cttctcaccc cttcatcacc ggcaccggcg
     6421 tctgcccggt ctcgtcgagg taatacgcca cccatttgtg gaagaactgg ttgcccggct
     6481 cgttgcggcc gaaggtctgg tgggtcatcg cgcccgattc cagccccgtc tgcaccttga
     6541 ggccaaggaa ataatcctcc tcctccacca catcgaagaa gaagtcgctc atttcgtcga
     6601 gcttcttcag cccctcttcc gtctccggct tggtggggaa gatgtagttc atcaccgtgg
     6661 tgttctgatt gacctccggc cccgggaaca gctgcgccag ctggcccatt tccggcgcca
     6721 ggaagaagct catgttgggg aagagcatgc ggatgaaatc atagcccttg ttttcctgca
     6781 gcccccattc ctcgcgcggc agatcatgca ggcgagtgat cgcattctgc gggaagccca
     6841 ggcggatatg cgggccaaag ccttcgtaat gggcgcggtt ggaagggctg cgggtggcca
     6901 ccgtgttggt gtgggcggcc tggaagtgat agccttccag atagccgtca taggccacct
     6961 tccagttggc gcccttcatc tcgcggcttt tgtggtaata ccacgtatcc agcttcagcg
     7021 cggcaaaatc ctcatacatg ccgcccagcc aggccaccgc atcgatcggc aggccggggg
     7081 tgaggatgac gaaaatgagg cccgccgcct cgtcgcaggg cagttcggtg aggtgcaggc
     7141 ggctcttgtc cacctcgcca aaggtgcccg cctcggcgat gccgatcagc tggccatcat
     7201 tggcataggt ccagccgtga tactggcagg caaagcggct gcaatggccc ttgccctcgc
     7261 tcaccatcag catggcgcgg tgcttgcaca cgttgaggaa ggcgtgcgcc ttgccatccc
     7321 tggtgcgggt gatcagcacc ggcaggccca tcacctgcat cgccttgtag tcaccggggt
     7381 tgggcagctc gatcgtcagc gccagcatca gcggcaggcg cttgaagatg cggtcgatct
     7441 ccttgtgcca gcgcccctcg tcgagatagt tctccaccgg cacctgcatc gtggccgaag
     7501 cctgatcggt ggtcttggtc tccacgaaat ggagcatgcg ctcagcgatc tcgccatatt
     7561 tcctgtagat cttggtcatc gccctcaccc tttcgcgttc agatagctgt cgagcgtctg
     7621 gtggaagtgg cggatgcgca cttcctgata gtcgcccagc tccaccttct ggttcttcga
     7681 cactttcagg ccctcatggg tgagctccat attggtcatg tcctgatcga ccacgccgcc
     7741 cagcacgccc agttccggcg catcagccca ggtctcgtgc tcggccagca aatggaaggg
     7801 cacgccatga gggcgtttgc cgccgcgctg atcttcgggg atgcgggcca ccacgcgcac
     7861 ttccatcagg cagtgatcct gatcgggcca gggccgccag cgatagacga tattgggcat
     7921 gaacccgccc cagggcgcga aattggggaa cacattgtag accagcgcat ccagcagctc
     7981 gctttccgac acatcggcat aatcgccgcc gaactgccgg gccgacacct cgcgcatgcg
     8041 cgcgcccagg gcctggcggg cgctggcgcc ttccggcacg gcgatggcgt aatggtcctt
     8101 ctcagggtcg taattgtcgg cgctgcggcc attgtatttc acgaattcat cgacgatcca
     8161 ctgctccggc agcccctgct ccagatgcgg gctgataacg ccgaagggtg tgtagttcac
     8221 attcacatgg cggcccagca catgataggc ggcattggca tcgccggtga aaggcatcag
     8281 ctgcggatgg gtgacaaagg cgtggtagct ttccatgaag gcttccatgg tgatcttcca
     8341 gttggccttc accaccttgc ccacccaggc caccgtcacg cagtcctcat gcttccagcg
     8401 cttgaagaac tcgggcagcg gggcgaggaa ttcctcgatc gaggggccct cggggttctc
     8461 gcgcaggaag acatagccgc cccagcgggc cacgctcgcc tcgggcagct gcagtttctc
     8521 gtcgctcaga tggccgaaat cccagcggca ggggatgccc cgcagcgaac cgtcggtgtt
     8581 ccaggcaaag ccgtggaagg ggcagacgaa ctggtcggcg ctgccgctgt cggtcttcag
     8641 cttgcggccg cggtgcaggc acacattgtg gaaggcgcgg atggagtcgt cgtcctggcg
     8701 caccagcagg aaggagcggc cggcgttctc gtaggtgaca taatcgccgg gctcgggcag
     8761 gtcttcctcg cgcgcggcaa actgccacac gttgggccac atcttttcct gttcggcgcg
     8821 gaagaaggcg gggtcgtaat agcgggcggc atcgatcgcc tcgctgccca gatagcggta
     8881 gcggtcctcg gccacgaagg cgggcggcgc cacatcatcc tcgcgcagaa tgtcttccca
     8941 gcttttgccg gggcagcgcg ccgggcggaa accctcgctt gcgtcaaaat cggccatata
     9001 ccctctccat atgtaatggc tgaaaggtag tggcaggggc tgcgcggcgg cttgatcgag
     9061 gtcaagaaaa gcgcccgcga gggcctgtga atagtgctag gatctttgtc taaaaccggc
     9121 gagaaagcga gccattgcgg gcataaccgg cgcatttgcc ggttatgccc gcaagccgga
     9181 tcagacgcgc ttgatctcgt ccgtctcgtg gatttccatg atctcgaaga cattgccgaa
     9241 aggatcgcgg caataggtgg cggcatagcc ggtcttgctg ccatcggggt gggtggtgat
     9301 gctgtcccag gccggctcgg catgcacgcg gatgccggca gccagcagcc gctcgtagca
     9361 ggccttaagg tcgctcacct gcacgccgaa atgggtgtag ccgaaattgt ggacgccgcg
     9421 gtcgggctcc tgcggggcgg atcgcggggc gtggtattca aacacctcga tatgggtgtt
     9481 gcccagccgc accatgaact gatcggcagc gctgcccttc agccccacca ccgcatcgat
     9541 ggccggattg tcgcgccagg ccagcggcgc cacttcttcc accccgccca gcagatcgat
     9601 gtaaaacgcc cgtgccttgg ccagatcggg cacggaaatg ccgatatggt gcacgccttc
     9661 aacgccaatg gccatcgctt ctctcccgcc cgccggcgct tgggcggccg gtcgggccat
     9721 tatgcgcggc agggggccgc cctgccccca tggcgaggcc agccccctgc cgcaggatca
     9781 gaactgcacg cgcttggtga agccgccatc caccaccaca ttggtaccgg tggtgtagga
     9841 agaggcaggg ctggcgagga agacaatggt cttggccact tcctccggcc cgccccagcg
     9901 gcccagggca aactgcgcct cggtggcatc gtaaaggccg ggggccagtt ccttgatcgt
     9961 ctcccaattg ccgccggcaa acttgatcgg cccgggcgac acggtgttga cgcggatgcc
    10021 cttgcccccc agcgcctggc tcagctggcc ggcatagacg atcagcgcgc ccttcagcgc
    10081 attgtagccc tgcggcacga tgaaggtttc caggcccgcg gtgctgccca tcagcacgat
    10141 gctgccggtg cccgaggcgg ccagctgctc ttccagcact tccacgccga tcaccgagcc
    10201 catgatgtca tagtcgaggc tcttgtgcca atcgccggtg gcgcccgcgc ccgaggtgga
    10261 gatcatcggc acgaagatgt cgcagccgcc cagcttttca gccgcaccct tcagccaggc
    10321 gcgataggca tcctggccgg cttccatgtc gaacgcctcg cccaccacct tgccgccgtg
    10381 gctggccagg gcagctgccg tttcggccac ttgctcggca ttccgcgagc agaaggccac
    10441 atcggcgccc tcggccgcga agagctccgc cgttgcccgg ccgatcccgc ggctgccgcc
    10501 ggtaaggata accttcttgc ccttcagacc cagatccatc ttgcactccc gctcgcgaaa
    10561 atttggcttg gatgccgttg ttgccccgca ggcaaggcca caccactgct cttttggcga
    10621 ggcaggcaca agccgggccg atgctaccca ccagcaatgc accaatcgcc ggccgcgcgc
    10681 ggcctgccat aagggagaga tgggaaaatg ggacgtttgg ccggcaagcg cgccgtggtt
    10741 ctgggcgcca gcagcagcga caacatgggc cagcacatcg cccgccgttt tctggccgaa
    10801 ggggccgagg tgctggtatc gggccgcaag ggcgaggtgc tggaagcctt cgccggcgag
    10861 accggctgcc agtggcaccc ctgcgacctg accgcgcccg ccagcgtggc cgcccttgcc
    10921 gatgccgccg cccaaaagct gggcggcatc gacattgccg tcaacgccac cggctggggc
    10981 ctgctgaagg gcttcctcga gaccacccat gaagacctgc tggagatgac cgccctgcag
    11041 ttcatcgggc cctaccattt ctatcaggcg atgatcggca agatggcctt cagcaagggc
    11101 gggcgcggcg gcgtgctgct gcagatctcc agcgccaccg ccaagatcat gctgaacgac
    11161 catgccgcct atatgggcac caaggccggc accgatcatg tgatccgctg cgtggcgcat
    11221 gaattcggcg ccgagggcat ccgcgccaat tcgatctcgc cgggcctcac cgacacgccg
    11281 atgaccgccc acgccaagac cgtgccgggc ctgttcgaaa gcttcctggc cggctatccg
    11341 ctgggccgca tcggcaccag cgacgatatc gccgcggccg ccgtgttcct cgccagcgac
    11401 gaatgtttca tgaccggaga aaacctgcag gtgaacggcg gcctcaccct gcgccgcaac
    11461 cccacccgcg aggaaatggc ggccgtgatc tcggccgccc acaacaaggg ctgatacaca
    11521 agatgacgac agatattccc gcaatcctgc gcgccgccga tattgcccgg tgggatcgcg
    11581 aggtggatgt ggtggtgatc ggccaggggg cggcgggcac ctgcgcggcc ctcgaagccc
    11641 accgcgccgg cgccgaggtg ctgatcgtgg agcgcgcctc tggcggcggc ggggcttcgg
    11701 cgatgtcctc gggcatctat tacctgggcg gcggcaccgc cgtgcagaag gcctgcggct
    11761 atgacgatga tgccgagcag atggccgctt tcatgatggc cagcaccgac cccacggacc
    11821 cgatggccgt gcatgccttc tgcgccgaca gtgccgccca tttcgactgg ttggaagccc
    11881 agggcgtgcc cttcgagcgc agcgccttca agggcaaggc ggtgttcctc aacaccaccg
    11941 aatgcctgat gtataccggc aacgaaaagc tctggcccta cagcgaggtg gcccgcccgg
    12001 ccccgcgcgg ccaccatgtg gcgggcaccg gcgagaatgc cggcgctgtg ggcatggccc
    12061 cgctgctgga acgctgcgcg caagaggaga tcccggcgct gtatgatgcg cgcgtgacgg
    12121 cgctggtgca ggatgccggc ggccgcgtgg tgggcgtgag cgtgaagcag agcgggggca
    12181 tgctgcatgt ggccgcgcgc aagggggtga tcatcgccgc cggcagcttc aacctcaacc
    12241 gcgagatgat cgcccacaac atcccgctga tcgccgaaac atcggagccg ctgggcattt
    12301 cctccaacga tggggcgggc gtgctgctgg gcctgtcggc cggcgctgcc accagcgcga
    12361 tggacgggat catccccacc gcctcgatct atccgcccgc gcagctgatc aaggggatca
    12421 tcgtcaacgc gcggggcgaa cgcttcgtgg cggaggattc ctatcacggc cgcaccgcca
    12481 atttcatcat ggagcagccg ggccagaagg cctatctcat cctcgattcc gaaatcttcg
    12541 cctatcccga gatcacctcg gcccgccacc ggctgatcga tggctgggaa acggtggcgg
    12601 aaatggaagc cggcatcggc cttcccgaag gcagcctggc caccacgctg gcgcgctata
    12661 acgccgatgc cgccgccggc aaggacagcc tgttccacaa ggcggccgac tggctgaagc
    12721 cgctggacca gggcccctat gccgccttcg acatcagctt cgacacctcg atctatctgt
    12781 tcatcacgct gggcgggctg aagaccgatg tggaaggccg ctgcctcgat ctttacagcc
    12841 agcccgtgcc cggcctctat gccgtgggcg ccagcgcggc gcatatcccg cgcaccggca
    12901 aggcctatgc cagcggcatg agcctggggc cgggctccta tttcggccgg cgcgcaggcc
    12961 gccatgccgc cggcgcgccc tggccgcagg ggtgaatgaa aagcacctgt gcggggcgcc
    13021 ctgccccgca caggtgctgg ccggcaatcg aaaaggggcg ccagggtatc cccccggcgc
    13081 cccttttcgt tgccggccag cgccagcttt ggaacctcag gccgcttcca gcttggcctt
    13141 gatatcggcg cgcagctgat ccttgcggat cttgcccgag gcggtgcgcg gaaaatcgct
    13201 gacgaattcg aaacgctcgg ggatcttctg cttggcaagg ccgctggcgg ccacatgggt
    13261 ggcaagctgg cccgcctcca ccgcgccggt ggcgatgaca taggcgcaca cgccctcgcc
    13321 cagccggtca tggggcatgg ccaccacgct ggcttccttc accagcggat gggtgtgcag
    13381 cacatcctcg atctccttgg ccgagatatt ctcgccgccg cggatgatga gatccttctt
    13441 gcggccggtg atcgtgaggg cgccgcgggc atccagcacg ccgaggtcgc ctgtgcggaa
    13501 gaagccatcc agggtgatcg cctcggcggt ctggccctcg tcggcatagc ccatcatcat
    13561 cgccgggccg cgcgccagaa tttcgccttc ctggccggcc ggcagcggct gctcctcggc
    13621 atcgacgatg cgcacctcgt aatccaccac ttcgccatcg gtagtggcgg ccagatcctc
    13681 ctcgccggca tggggccagc ccagcgtcac cagcggcact tccgaggcgc caaacacgcg
    13741 gaacggccgg caatgggcaa aggccgcgcg cgccgccggg atcagctctg gcggcaccgc
    13801 cgcgccgcca caggcaaaga agcggaacga gggcagctgc gtgcccgccg cccgggcagc
    13861 ggcagccagc tccaccagga agggcgtggc cgccaccgtg cccaccagct ggtgccgctc
    13921 gatcagggcc agcgcctcat cggccttcca ggcttccatc agcacgctgc ggatgccgca
    13981 cagcagcggc gcctccagcc cgttggcata gcccgagaca tgggtgacag gcgatggcat
    14041 cagcgtggcc tcgccgggcg caacgcccca gtgggcgtgg ctcttgcgca gcacatgctg
    14101 caaggtggtg tggctgtgca gcacgccctt gggccggccg gtggtgcctg aggtgtagag
    14161 caccatcttc acgcccatcg gatcgaccgc ggggcgggca aaggccttgc cgcgcccttc
    14221 ggccagcagg gcggcgtaat cgtcaggccc gctgccgcgc acggtgaaca catgggccag
    14281 atcgggcaga tcggcccgga tgcgcgctgc catcgcgcca aaatcatagt tgcggaaaga
    14341 ggcggcgaca aagattgccc gcgcccggca atcggccagc atcaggcgca cttcatggtc
    14401 gcggtagatc ggcacgatgg ggttgacgat gaggcccgac agcgccgcgg ccaggttgat
    14461 caccgccgcc tcgcgccagt tgggcacctg gaaggccacc acatcgcccg ggcgcaggcc
    14521 ccgctcatgc agcgctactg ccagcgcctc ggcatcgccc accagctcgg cgcggctcag
    14581 ctgcgcctcg ccttccacaa aggcgatgaa gccgggatcg gcc
//
LOCUS       DASRYE010000320         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_172253,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000320 DASRYE010000000
VERSION     DASRYE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172253"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..3611
                     /gene="dnaE"
                     /locus_tag="VFF98_16575"
     CDS             12..3611
                     /gene="dnaE"
                     /locus_tag="VFF98_16575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZU65305.1"
                     /translation="MAHAPFVPLRVCSNYTMLDGAIDPKAAAKLAKKRGFPAFAITDK
                     NGLYCAPAFAAAARGEGVQPIIGAMLAVAREEGPSQGVFGGPVAPQIDWLALLVQNET
                     GWLNLCHLVSHAHLERPLEFDPHVRLADLEGWTQGLICLTCGADGALARLYAEGQEEA
                     AGRYADRLQGLFDGRLYIEIARTGQAAEEASEDALIALAYARDIPLVATNPAHFADAS
                     GFSAHDAMLCIAGSTHVDVVDRPRSNREFWVKPAETMASLFADLPEALANTLVVAQRC
                     AYAPPKRKPLLPSLAGDKEGEAKMMADKAREGLAQRLAPYWPGVAEAQIDAALAEQGE
                     ARRAAWAETLAPQGVGEDFLDYADRLDFETGIINRMGFAGYFLIVADFIQWAKQNDIP
                     VGPGRGSGAGSLVAWSLTITDLDPLKLGLLFERFLNPERVSMPDFDIDFCETRRGEVI
                     RYVQAQYGADHVAQIITFGKLKARAVLRDTGRILQMSYGQVDRLTKMVPNHPTDPWSL
                     ERALNGVMELKREYDQDPDVKRLIDLAMQLEGMPRNSSTHAAGVVIGDRPLEKLIPLY
                     RDPRSDMPVTQFDMKFVEDTGLIKFDFLGLKTLSVLRKAVDLLKRRGITIDLGALPWD
                     DGDVYTLLQRGDTVGVFQLESEGMRRTLAAVKPTNFGDIIALVSLYRPGPMDNIPLFG
                     QRKNGLAPIEYPHDKLSGILAETYGIFVYQEQVMQAAQILAGYSLGDADLLRRAMGKK
                     VQAEMDAQRLRFVEGCKAVSAIEKDKANELFDIIDKFAGYGFNKSHAAAYALLAYQTA
                     WFKAHYPQEFYAAAMCFDMHQSEKLAVFVDDMRRNGFTIAGPCLNHSEAEFTVEQTAD
                     GYAVRYALAGIRNVGEKAMEQVVAEREANGPFRDLEDLFSRVPYGALNRRMLEGLAAS
                     GAFDALEANRARVLANADRLLAVAEEAERARTSGQHGLFGGEPGGAGALRLLDTPAWS
                     RQEQMAKERENFGFYFAAHPVEQWRAVASANGARSHAALMTQWSDGAFAGGRQQAMMA
                     AMVENVQKRKTKRGKDFVMADFSDQSGQFSASCFEESVVDNFVEWARESACVLLTVEL
                     DSPSPEEPPRVTVRGAQALSEVRAVARMLLSLEVERAEAFAELAAMLPRTAGARGEVE
                     VSVQALGEGTTRLRLGRDFLLDGELAEAVAAIEGVKGVSLAPIRGGAKLRLVA"
     gene            complement(3608..4708)
                     /locus_tag="VFF98_16580"
     CDS             complement(3608..4708)
                     /locus_tag="VFF98_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZU65306.1"
                     /translation="MSGTASRLPQPRGRGACSGAASARFGLPARAIDGDWLDHALAED
                     DLPPLRTTVTEEAARRIIARNASPDIPFSQSINPYRGCEHGCIYCYARPTHAWLDLSP
                     GLDFESRLFAKPAAARLLRRELARPGHVPSPIALGTNTDPYQPIEARYRLTRQILEVC
                     LETGHPVTITTKSDRVLADLDLLAALAAQGLAAVALSVTSLDPALSRRLEPRAASPAK
                     RLAALAALAGAGVPAMVNVAPIIPAITDHMLEEVLAAAAAAGARHASWIALRLPHEVA
                     PLFREWLEAHFPDRAAKVMHQVMAMRGGRDYDARFFQRMRPEGPWADLVRARFRRAAR
                     RLGLNTQRLQLDESRFRAPARAPANNGQLALF"
     gene            4886..>5229
                     /locus_tag="VFF98_16585"
     CDS             4886..>5229
                     /locus_tag="VFF98_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZU65307.1"
                     /translation="MDQAQAQAWLGAYLQRIGLGHVPPPTPEGLAQVQRAHRLAIAFE
                     NLDILLGRGIHLPLDQVAAKLLGQRRGGYCFEQNGLFGAMLGAMGLANRPLLARVWVQ
                     AGDSMPARTHTLR"
BASE COUNT          867 a         1669 c         1867 g          826 t
ORIGIN      
        1 atctggccgg catggcccat gcacccttcg ttcccctgcg cgtctgctcc aactacacca
       61 tgctggatgg cgcgatagac cccaaggccg ccgccaagct ggccaagaag cgcggctttc
      121 ccgcctttgc catcaccgac aagaacggcc tctattgcgc cccggccttt gccgcggcgg
      181 cgcggggcga gggcgtgcag ccgatcatcg gcgcgatgct ggctgtggcg cgcgaggaag
      241 ggcccagcca gggcgtgttt ggcggcccgg tggcgccgca gatcgactgg ctggcgctgc
      301 tggtgcagaa tgagacgggc tggctgaacc tgtgccacct cgtctcccac gcccatctcg
      361 aacggccgct ggagtttgac ccccatgtgc gcctggccga tctggaaggc tggacgcaag
      421 ggctgatctg cctcacctgc ggcgccgatg gtgcgctggc ccggctctat gccgaggggc
      481 aggaggaggc cgccggccgc tatgccgatc ggctgcaggg gctgttcgat ggccggctct
      541 acatcgagat cgcccgcacg gggcaagcgg cggaagaggc cagcgaggac gcgctgatcg
      601 ccctggccta tgcccgcgac atcccgctgg tggccaccaa cccggcccat ttcgccgatg
      661 ccagcggctt ttccgcgcat gatgccatgc tgtgcattgc cggctccacc catgtcgatg
      721 tggtggacag gccccgttcc aaccgcgaat tctgggtgaa gccggccgag acgatggcca
      781 gcctctttgc cgatctgccc gaggcgctgg ctaacacgct ggtggtggcg cagcgctgcg
      841 cctatgcgcc gcccaagcgc aagcccctgc tgcccagcct tgccggcgac aaggaaggcg
      901 aggccaaaat gatggccgac aaggcccgcg aaggactggc gcagcgcctg gccccctatt
      961 ggcccggcgt tgctgaagcg cagatcgatg ccgcgctggc agagcagggc gaggcacgcc
     1021 gcgccgcctg ggctgaaacg ctggcgccgc agggcgtggg cgaggatttc ctcgattacg
     1081 ccgatcgcct cgatttcgag accggcatca tcaaccgcat gggctttgcc ggctacttcc
     1141 tgatcgtggc cgatttcatc cagtgggcca agcagaacga cattccggtg gggccggggc
     1201 gtggttcggg cgcgggcagc ctggtggcct ggtcgctcac catcaccgat ctcgatccgc
     1261 tgaagctggg cctgctgttc gagcgcttcc tcaaccccga acgcgtctcg atgccagact
     1321 tcgacatcga cttctgcgaa acccggcgcg gcgaggtgat ccgctatgtg caggcccagt
     1381 atggcgccga ccatgtggcc cagatcatca ctttcggcaa gctgaaggcc cgcgcggtgc
     1441 tgcgcgacac ggggcgcatc ctgcagatga gctatggcca ggtggaccgc ctgaccaaga
     1501 tggtgcccaa ccaccccacc gatccgtgga gcctggagcg cgcgctgaac ggcgtgatgg
     1561 agctgaagcg cgaatacgac caggatccgg atgtgaagcg gctgatcgat ctggccatgc
     1621 agctggaggg catgccgcgc aacagttcca cccatgccgc gggcgtggtg attggcgacc
     1681 ggccgctgga aaagctgatc ccgctctacc gcgatccgcg ttcggacatg ccggtgacgc
     1741 agttcgacat gaagttcgtg gaagacaccg gcctcatcaa gttcgacttc ctcgggctga
     1801 agacgctgtc tgtgctgcgc aaggcggtgg atctgctgaa gcggcgcggc atcaccatcg
     1861 atctgggcgc gctgccctgg gatgacgggg atgtgtatac gctgctgcag cggggcgata
     1921 cggtgggcgt gttccagctg gaatcggaag gcatgcggcg cacgctggcg gcggtgaagc
     1981 ccaccaattt cggcgacatc atcgcgctcg tctcgctcta ccggccgggg cccatggaca
     2041 atatcccgct gttcggccag cgcaagaatg gcctggcgcc gatcgaatac ccgcatgaca
     2101 agctctcggg cattctggcc gaaacctacg gcatcttcgt ctatcaggaa caggtgatgc
     2161 aggccgcgca gatcctcgcc ggctattcgc tgggcgatgc cgacctgctg cgccgcgcga
     2221 tgggcaagaa ggtgcaggcg gaaatggacg cccagcgcct gcgcttcgtg gaaggctgca
     2281 aggcggtctc ggccatcgag aaggacaagg ccaacgaact gttcgacatt atcgacaagt
     2341 tcgccggcta tggcttcaac aagtcgcacg cggcggccta tgccctgctg gcctatcaaa
     2401 ccgcctggtt caaggcgcac tacccccagg aattctatgc cgcggccatg tgtttcgaca
     2461 tgcatcagtc ggaaaagctg gcggtttttg tcgacgacat gcgccgcaac gggtttacca
     2521 ttgccggccc ctgcctcaac cattcggaag ctgaattcac ggtcgagcag acggccgatg
     2581 gctatgccgt gcgctatgcg ctggcgggca tccgcaatgt gggtgaaaag gcgatggagc
     2641 aggtggtggc cgagcgcgag gccaacgggc cgttccgtga tctggaggat ctgttctccc
     2701 gcgtgcccta tggcgcgctc aaccggcgga tgctggaagg gctggcggcc tcgggcgcgt
     2761 tcgatgcgct ggaggccaac cgtgccaggg tgctggccaa tgccgatcgc ctgctggccg
     2821 tggccgaaga ggccgagcgc gcccgcacca gcggccagca tggcctgttt ggcggcgagc
     2881 cgggcggcgc cggcgcgctg cgcctgctcg acacgccagc ctggagccgg caggagcaga
     2941 tggccaagga gcgcgagaat ttcggcttct atttcgccgc ccatccggtg gagcagtggc
     3001 gggcggtggc cagcgccaat ggcgcgcgca gccatgccgc gttgatgacg caatggagcg
     3061 atggcgcctt tgccggcggc cgccagcagg cgatgatggc agcgatggtg gagaatgtgc
     3121 agaagcgcaa aaccaagcgc ggcaaggact tcgtcatggc cgatttctcc gaccagtcgg
     3181 gccagttctc ggccagctgc ttcgaggaat cggtggtcga caattttgtc gaatgggcgc
     3241 gggaaagcgc ctgcgtgctg ctgacggtgg agctggacag ccccagcccc gaggagccgc
     3301 cgcgcgtgac cgtgcgcggt gcccaggcgc tgagcgaggt gcgggcggtg gcgcgcatgc
     3361 tgctctcgct ggaggtggag cgggccgagg cctttgccga gctggccgcc atgctgcccc
     3421 gcaccgccgg cgcgcgcggc gaggtggaag tgagcgtgca ggcgctgggc gagggcacca
     3481 cccgcttgcg gctgggccgc gatttcctgc tggatggcga actggccgag gcagtggcgg
     3541 cgatcgaggg ggtgaagggg gtttccctgg cgccgatccg cggcggggcg aaactgcggc
     3601 tggtggccta gaacagcgcc agctggccat tgttggcagg ggcgcgggcg ggggcgcgaa
     3661 agcggctttc gtccagctgc aggcgctgcg tgttcagccc cagccggcgt gccgcgcggc
     3721 gaaagcgcgc ccgcaccaga tccgcccagg gcccttccgg ccgcatccgc tggaagaagc
     3781 gggcatcgta atcgcgcccg ccgcgcatcg ccatcacctg atgcatcacc ttggcggcgc
     3841 gatcggggaa atgggcctcc agccattcgc ggaacagcgg cgccacttca tgcggcaggc
     3901 gcagggcaat ccagctggca tggcgggccc cggcggcagc ggccgcggcc agcacctctt
     3961 ccagcatatg atcggtgatg gcagggatga tcggcgccac attcaccatc gccggcacgc
     4021 ccgcgccggc cagcgcggca agggcggcca ggcgcttggc ggggctggcc gcgcgcggct
     4081 ccagccggcg cgagagggcc ggatcgaggc tggtgaccga aagcgcgacg gctgccagcc
     4141 cctgcgccgc cagcgccgcc agcaggtcca gatcggccag cacgcgatcg gatttcgtcg
     4201 tgatggtgac aggatggccc gtctccaggc acacttcgag gatttgccgg gtcagccggt
     4261 agcgcgcttc tatcggctga tagggatcgg tgttggtgcc cagcgcgatg gggctgggca
     4321 catggccggg gcgtgccagt tcgcgccgca gcaggcgggc ggcagcgggc ttggcaaaca
     4381 gccggctctc gaaatcgagc ccgggcgaga gatccagcca ggcatgagtg ggccttgcat
     4441 agcaatagat gcagccatgt tcacacccgc gataggggtt gatggactgg ctgaagggaa
     4501 tatccggcga ggcgttgcgc gcgatgatgc ggcgcgcggc ttcctcggtc acggtggtgc
     4561 gcaagggggg caggtcgtcc tcggccaggg catggtccag ccagtcgcca tcgatggcgc
     4621 gcgcgggtag gccaaagcgg gccgatgccg cccccgaaca ggcgccgcgc ccacggggct
     4681 gcggcaggcg agaagccgtt ccagacataa cggatcatga acataaaaag aacgtatgcg
     4741 caataaggaa aaccgccgtc cggccaatcc actcgtccca agccgcttgc agggggcttt
     4801 gcggcaagat aggcgcaagg ggccggcgcg ctgattcgca ctgcgccggc atcggcacag
     4861 gcgaaagcgg gcacacggtt cgggcatgga tcaggcacag gcgcaggcat ggctgggggc
     4921 ctatctgcag cgcattggcc ttggccatgt gccgccgccc acgcccgaag ggctggcgca
     4981 ggtgcagcgc gcgcaccggc tggcgatcgc gtttgaaaac ctggatatcc tgctgggccg
     5041 gggcattcac ctgccgctcg atcaggtggc ggccaagctg ctgggccagc ggcgcggggg
     5101 ctattgcttt gaacagaacg gcctgttcgg cgccatgctg ggcgccatgg ggctggccaa
     5161 ccggccgctg ctggcgcggg tgtgggtgca ggcgggcgac agtatgcctg cgcgcacgca
     5221 cacgctgcg
//
LOCUS       DASRYE010000321        15991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_66716,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000321 DASRYE010000000
VERSION     DASRYE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66716"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1551
                     /gene="nifD"
                     /locus_tag="VFF98_16590"
     CDS             52..1551
                     /gene="nifD"
                     /locus_tag="VFF98_16590"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HZU65308.1"
                     /translation="MSVSTPTNIAEVKERNRALIQEVLSVYPEKTAKRRAKHLNVHEE
                     GKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARR
                     NYYVGTTGIDTFGTMQFTSDFQEKDIVFGGDKKLAKIIDEIEQLFPLNKGISVQSECP
                     IGLIGDDIEAVSKNKSKEYGGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKSEG
                     KPVAFEPSSYDVAIIGDYNIGGDAWSSRILLEEMGLRVIAQWSGDGTIAELEATPKAR
                     LNVLHCYRSMNYISRHMEEKYGIPWVEYNFFGPSKIEESLRKIASYFDEKIQAGAEAV
                     IAKYKPLMQAVIDRFRPRLAGKKVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHND
                     DYQRTAQHYVKDGTLIYDDVTGYEFEKFVETVQPDLVGSGIKEKYVFQKMGVPFRQMH
                     SWDYSGPYHGYDGFAIFARDMDMAINSPVWKLPQAPWKADPDYVAIAAE"
     gene            1614..3173
                     /gene="nifK"
                     /locus_tag="VFF98_16595"
     CDS             1614..3173
                     /gene="nifK"
                     /locus_tag="VFF98_16595"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HZU65309.1"
                     /translation="MPQDAEKIKDHFELFRGDEYKDMIANKRAHFENRHADAEVERVR
                     EWAKTEEYKELNFAREALTVNPAKACQPLGAVFVAVGFEGTVPFVHGSQGCVAYYRSH
                     FSRHFKEPTSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGD
                     DLNAFIKTAREKGSIPAEYPVPFAHTPAFVGSHITGYDNAMKGVLEYFWDGRAGTIDK
                     VARVPNGKINFLGGFDGYTVGNLREVKRIFEMMGVEYTILGDSSDVWDTPTDGTFRMY
                     DGGTTLEDAEKAVHARATISMQEFCTEKTLPMIAAHGQETVAFNHPIGIEATDRFIME
                     VARLAGVAIPDALARERGRLVDAVADSNAHVHGKKFAIYGDPDLCYGLAQFVMELGGE
                     PVVVLATNGGKGWAKKMQALFDSSPFGAGCKAYPGKDLWHMRSLLFTEKPDYLIGNTY
                     GKYLERDTGVPLIRIGFPIFDRHHKHRYPVWGYQGGLNVLVTILDRIFEHMDATTNVP
                     SQTDYSYDIIR"
     gene            3224..4888
                     /gene="nifE"
                     /locus_tag="VFF98_16600"
     CDS             3224..4888
                     /gene="nifE"
                     /locus_tag="VFF98_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HZU65310.1"
                     /translation="MNSLSQTIQDVFNEPGCDKNQAKSEKERKKGCTKQLQPGGAAGG
                     CAFDGAKIALQPITDVAHLVHGPIACEGSSWDNRGAKSSGPQLYRTGFTTDMGENDIV
                     FGGEKRLFRAIREIRDKYAPPAIFVYQTCVPAMIGDDIEAVCKAASAKFGTPCIPILS
                     PGFVGPKNLGNKLAGEALLKHVIGTEEPEFTTPYDINIIGEYNLAGELWQVKPLLDEL
                     GIRILSCISGDARYREVAWSHRAKAAMMVCSKAMINVARKMEERYAIPFFEGSFYGIG
                     DMSESLRQIATLLIAQGADAELMARTEAVIAREEQRAWAAMEPYARRLAGKRVLLITG
                     GVKSWSVVAALQEAGMEVVGTSVKKSTDEDKEKIRQIMGKDAHMIDDMTPREMFRMLK
                     AAEADIMLSGGRSQFVALKATMPWMDINQERHAAFAGYHGMVEMVKEIDRTLANPVWE
                     QVRLPAPWHAEPGWQDLALAENPAPVAETPAPVAGAAPAPDPRARAVCHCKAVSVGMI
                     ADAVALGHTTREAVARATRASTGCGGCGPWVDKAIREALPSASAAA"
     gene            4892..6277
                     /gene="nifN"
                     /locus_tag="VFF98_16605"
     CDS             4892..6277
                     /gene="nifN"
                     /locus_tag="VFF98_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HZU65311.1"
                     /translation="MARVEKSAKACTVNPLRMSQPIGGALAFMGMEGTMPLLHGSQGC
                     TSFGLVLFVRHFREAIPLQTTAMSEVATVLGGYDNVEQAVANIAARTKPALIGICSTG
                     VTEIKGDDLAGFVQQIRARRPDLADVALVHCSTPDFAGAFQDGWARAVTAMVEQLVEP
                     VAPNARAREAGRVNVLPGCHLTPGDIDALRDIFTDFGLTPTFLPDLSGALDGHIPQDF
                     TPTTLGGTSLAAVRAMGQAAHTIAIGAQMGPAAAALSARTGVPHRLFDRLTGLAPCDA
                     FIAHLARISGRPVPAKYRRQRGQLIDAMLDGHFHIGGRRIALGAEPDLLFALGSMLAE
                     MGGQIVAAVTTTASPLNARLPAERVTIGDLEDLEAQARDGDAQILITHSHGRQAAARL
                     VLPHFRIGLPLFDTLGAGHLVSVGYKGTRDLVFAIGNMLMHSHHEAAPEDWRDAWNAH
                     PHTPHRKEAAQ"
     gene            6274..6675
                     /gene="nifX"
                     /locus_tag="VFF98_16610"
     CDS             6274..6675
                     /gene="nifX"
                     /locus_tag="VFF98_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HZU65312.1"
                     /translation="MKVAFATQDLQRIDAHFGWAKNLVIYDLTPEGWTLDRVESFEGD
                     LKEDGDEDKLAPKLAALQGVAILVVAAIGGSGAARVVAQNIHPMKVAEPEPIEAMLER
                     LREVLRGTPPPWLRKVMARTDRDFDFEEEEA"
     gene            6678..7163
                     /locus_tag="VFF98_16615"
     CDS             6678..7163
                     /locus_tag="VFF98_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HZU65313.1"
                     /translation="MADSQTLHATGGAAPAEPAITGFAADLVRQLRATDTHGAWDRRS
                     DWSLLEPFIVDREKRRAIPIIGDPDPDTIWRLELFYGALCLAIEKRCGKMVSPLMKLS
                     HEGFGRMVLAAGRLIVINKSLRDVHRFGFDSLEKLNGEAEKLIGQALEMIARFPAVAD
                     Y"
     gene            7175..7393
                     /locus_tag="VFF98_16620"
     CDS             7175..7393
                     /locus_tag="VFF98_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HZU65314.1"
                     /translation="MADAEALKAEVKKLSAQAMQAKMDLHDLSEELPVGVEKVPQVAQ
                     RVVAAYAALAERKAALQALAETAAGTAA"
     gene            7390..7677
                     /gene="fdxB"
                     /locus_tag="VFF98_16625"
     CDS             7390..7677
                     /gene="fdxB"
                     /locus_tag="VFF98_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HZU65315.1"
                     /translation="MSGLLTRGGQPWAPAYLLAIDPALCIGCGRCFKVCGRGVMGLHG
                     LTEEGALVADDEDDDEIVKRVMALDDAGACIGCGACARVCPTNCQTHGAAG"
     gene            7677..8318
                     /locus_tag="VFF98_16630"
     CDS             7677..8318
                     /locus_tag="VFF98_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HZU65316.1"
                     /translation="MAQGPALYAALVAAGQAAGADPFDCHVAAAILAMAAGEEAGAPA
                     EGCGSGLDAGERAELSALLFGGAALAGPDPCAMPQQEQTLRDILWMNAGSGSRLQRLL
                     ARMIARRALRPNHLWQDLGLAHRGELSALMQRHFPRLAARNAADMKWKKFFYRMMCND
                     AGFALCAAPVCSECDDFDACFGGEEGEALLARLANGRGLPPAALPAPSNGAHA"
     gene            8315..8755
                     /locus_tag="VFF98_16635"
     CDS             8315..8755
                     /locus_tag="VFF98_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HZU65317.1"
                     /translation="MSEPVCSSPPDATPAPPAEPPFDAAGEAAIMAMVRRFYDLCLAD
                     DLLGPMFRAVIDDFDAHYGIVADFWSHALLGTHRYQRGTPYSHHTHLTVSEAHFTRWM
                     AAFGQAVGEILPAPLADKAMQRAAHMTASFRMGLLPLPEPGPRP"
     gene            8752..9177
                     /locus_tag="VFF98_16640"
     CDS             8752..9177
                     /locus_tag="VFF98_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU65318.1"
                     /translation="MNAPAPARRPAVYAVCALEEIPNRRARAFVLARAGADGAPRPWP
                     IFILRWGRHVRAYENRCPHQGVHLDWERGEFLDGEGIRIQCGKHGALFDLGTGACVAG
                     PCLGEGLVPIEAVIEEGEICLAGVDLLEDEDEDSGQGAE"
     gene            9209..9424
                     /locus_tag="VFF98_16645"
     CDS             9209..9424
                     /locus_tag="VFF98_16645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZU65319.1"
                     /translation="MPLFDIACPACGATSEVLARAADPVVCPACGSPEARRLVAQVAA
                     PGTSRALAAAGREAARKAGHLSHFKGG"
     gene            complement(9479..10090)
                     /locus_tag="VFF98_16650"
     CDS             complement(9479..10090)
                     /locus_tag="VFF98_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65320.1"
                     /translation="MSTMDAVRLEDTALGRLNAAHRLLNAVLKEPLPAAGTFGFRGDI
                     ALAFQDQVADEARPPAYALEQVLARADAATGKIPVLVGYLHNFAWLKDVAEVLADCLD
                     PAGSYVLFANNIDFLKKYRVPLGGSGATAYVLPLDESTVWKETLELVDVDKNDVKKMS
                     GAQKLAHVLDKVAAFKAPYPELSYEEGVASMAPVRNRNENRPV"
     gene            10412..10732
                     /locus_tag="VFF98_16655"
     CDS             10412..10732
                     /locus_tag="VFF98_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZU65321.1"
                     /translation="MIFLTDSAAQAVRTAIGQAASAPAGLRIQVEAGGCAGYKYMMGL
                     VAEPRPDDAVVKEGDVAVFIDPASQPLIAGTTIDFVVALEGSGFTFENPNATNSCSCG
                     KSFG"
     gene            10746..11666
                     /gene="nifU"
                     /locus_tag="VFF98_16660"
     CDS             10746..11666
                     /gene="nifU"
                     /locus_tag="VFF98_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HZU65322.1"
                     /translation="MWEYSEKVKDYFFNPKNSGILEDADGVGDVGAISCGDALRLMIK
                     VDAPSQTITDARFQTFGCGSAIASSSALTELIIGKTIDEAMGLTNQDIADFLGGLPPE
                     KMHCSVMGQEALQAAIANYRGEAWVDDHEEGALICKCFGVDEGMIERTVRTNGLTSIE
                     EITNYTKAGGGCSTCAEGIEGVLERVNAAMVAEGLLAADRAFHAGVVPLPVKARKVVR
                     TEAPAARALSNLQKIRLIEETIAQIRPSLQRDGGDCELIDVEGNRVLVRLTGACVGCH
                     LSGATIEGVQARLVEAVGVPLLVVPVSAMH"
     gene            11680..12888
                     /gene="nifS"
                     /locus_tag="VFF98_16665"
     CDS             11680..12888
                     /gene="nifS"
                     /locus_tag="VFF98_16665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZU65323.1"
                     /translation="MSAQPVYLDNNATTRTDPAVVTAMLPFFTEQFGNASSMHAFGAE
                     VGGAITHARRALADLLGAAFDHEIVYTGGGTEADSTAILSALETQGQRNEVITSAVEH
                     PAVLSLVAHLEKTGRARVHIIPVDARGRLDMEAYAKALSERTAIVSIMWANNETGTLF
                     PVERLAEMAHGAGALFHTDAVQAVGKIPIDLKSTAIDMLSLSGHKLHGPKGIGALYVR
                     KGVRLRPLIRGGHQERGRRAGTENAPAIIGLGKAAELAAAHMAEEQTRVKALRDRLEA
                     GLLAAIGNSFVTGDVESRLPNTCNIAFEYIEGEAILLLMNREGIAASSGSACTSGSLE
                     PSHVLRAMNVPYTAAHGAIRFSFSRENGPEDVARVLEAMPPIIARLREMSPFWRERDQ
                     HTAAFNPTYA"
     gene            12911..14050
                     /gene="nifV"
                     /locus_tag="VFF98_16670"
     CDS             12911..14050
                     /gene="nifV"
                     /locus_tag="VFF98_16670"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HZU65324.1"
                     /translation="MPCQAPSVIINDSTLRDGEQAPGIAFTPAEKLAIALALDAAGVD
                     EIEAGTPAMGEAELAAIRDIASQARRAAVIPWGRLTRADADLAARVGAGRVHLSVPVS
                     DRQIRAKFGRHGGGGRLDVLARIADVVPYALDRGMRVSVGGEDASRADPDFLARVVVA
                     IEEAGGHRFRYADTLGVLDPFRVCDAFAGLARETDIELEFHGHDDLGLATANTLAAVR
                     GGASHASVCVLGLGERAGNAPLEEVVTALGETLGRRTGVDPARLGGLAELVARAACRP
                     IPDNKPIVGASVFAHESGIHVSGLLRDAGTYEALAPERFGRRRQIVLGKHSGRSAVRS
                     VLHTIGLDAEHERLEAILAAVRARAVATKRPVGLADLAALYEQTA"
     gene            14047..14775
                     /gene="cysE"
                     /locus_tag="VFF98_16675"
     CDS             14047..14775
                     /gene="cysE"
                     /locus_tag="VFF98_16675"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZU65325.1"
                     /translation="MRAPGLVALIAGDIACVRARDPAANSRIEIALTYPGVHAIIWHR
                     LAHRLWRRGWRFAARFLGWLARLLTNVDIHPAATIGQRFFIDHGAGVVIGETAEIGDD
                     VTLYHGVTLGGTSWQGGKRHPTLGDGVLVGCGAKILGPIGVGAGARVGANSVVIEDVP
                     PGMTVVGIPGRVVRAPRERRRIGERIDLDHHLMPDPVGTAIAELIDRIDFLEARLAHR
                     DRALPVRSRPAHSAATGENCHEPA"
     gene            14762..15109
                     /gene="nifW"
                     /locus_tag="VFF98_16680"
     CDS             14762..15109
                     /gene="nifW"
                     /locus_tag="VFF98_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase stabilizing/protective protein NifW"
                     /protein_id="HZU65326.1"
                     /translation="MSLLDRLQALSSAEDFFALLDVPYDEAVVRVARLHILRRMGQYL
                     RGSEAEGAFDGLDEVALQGLCRAHLAQAYADFVASSPIAERIFKVHQEAIAPKPEPNR
                     PFVPLTALTGGIA"
     gene            15106..15972
                     /locus_tag="VFF98_16685"
     CDS             15106..15972
                     /locus_tag="VFF98_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZU65327.1"
                     /translation="MTDPLHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLF
                     ALEEALRLRDRLGGVVTVLCMGPMTAEDSLRKALTFGADRAVLLSDRYFAGSDTLATS
                     YALAMALAQIGREFGPPQIIFTGKQTIDGDTAQVGPGIARRLGFNQLTYISRIREVDP
                     AGGRICVERRAEGGVQVLETRLPVLITMLEATNEMRRGAIETALAAARAQVTVWNAAS
                     VGIEDLTKCGLRGSPTIVKKVFAPGARAEKAAQIALGDTPEATAHAAIEAIFARLPGL
                     EAQLFETAAASC"
BASE COUNT         2701 a         5128 c         5611 g         2551 t
ORIGIN      
        1 gcagacagcg ccggcgggcc ccgggaaaac ccccgcagga ggacccaaga gatgagcgtc
       61 tcaaccccaa ccaacatcgc agaagtgaag gaacgcaacc gggcgttgat ccaggaagtc
      121 ctgtcggtct accccgagaa gaccgccaag cgccgcgcca agcacctgaa cgtgcatgaa
      181 gaaggcaaga gcgattgcgg cgtgaaatcg aacatcaagt cgatccccgg cgtgatgacg
      241 atccgcggct gcgcctatgc cggctccaag ggcgtggtgt ggggcccgat caaggacatg
      301 atccacatca gccatggccc ggtgggctgc ggccagtata gctgggcggc gcgccgcaac
      361 tattacgtgg gcaccactgg catcgacact ttcggcacga tgcagttcac ctccgacttc
      421 caggagaagg acatcgtctt tggcggcgac aagaagctgg ccaagatcat cgacgagatc
      481 gagcagctgt tcccgctcaa caagggcatc tcggtgcagt ccgaatgccc gatcggcctg
      541 atcggtgacg atatcgaggc ggtgagcaag aacaagagca aggaatatgg cggcaagacc
      601 atcgtgccgg tgcgctgcga gggttttcgc ggcgtgtcgc agagccttgg ccaccatatc
      661 gccaatgacg ccatccgcga ctgggtgttc gacaagagcg agggcaagcc ggtcgctttc
      721 gagccgtcgt cctatgatgt ggcgatcatc ggtgattaca acatcggcgg cgatgcctgg
      781 tccagccgca tcctgctgga ggagatgggc ctgcgggtga tcgcccagtg gtctggcgat
      841 ggcaccatcg ccgagctgga ggcgaccccc aaggcgcggc tcaacgtgct ccactgctat
      901 cgctcgatga actacatcag ccggcacatg gaagagaaat acggcattcc ctgggtggaa
      961 tacaatttct tcgggcccag caagatcgag gaaagcctgc gcaagatcgc cagctatttc
     1021 gacgagaaga tccaggccgg cgccgaagcc gtgatcgcca aatacaagcc gctgatgcag
     1081 gcggtgatcg acaggttccg cccgcgcctc gccggcaaga aggtgatgct gtatgtcggc
     1141 ggcctgcgcc cgcgccatgt catcggtgcc tatgaggacc tcggcatgga agtggtcggc
     1201 accggctacg aatttggcca caacgacgat taccagcgca ccgcccagca ctatgtgaag
     1261 gatggcacgc tgatctacga cgatgtcacc ggctatgaat tcgagaaatt cgtggaaacg
     1321 gtgcagcccg atctcgtcgg ctccggcatc aaggagaagt acgtcttcca gaagatgggc
     1381 gtgcccttcc gccagatgca cagctgggat tattccggcc cctatcacgg ctacgacggc
     1441 tttgcgatct tcgcgcgcga catggacatg gcgatcaact cgccggtctg gaagctgccg
     1501 caggcgccct ggaaggccga tcccgactat gtcgccattg ccgctgaata agcgctgatc
     1561 ccgggcgggc cggcccaccg gccccccacc tttttgccaa acaggaggcc accatgcccc
     1621 aggacgctga aaagattaag gatcacttcg agctgtttcg gggtgacgaa tataaggaca
     1681 tgatcgccaa caagcgggcg cacttcgaga accgccatgc cgacgccgaa gtggagcgcg
     1741 tgcgcgaatg ggccaagacc gaggaataca aggagctgaa cttcgcccgc gaggcgttga
     1801 cggtgaaccc ggccaaggcc tgccagccgc tgggcgcggt gtttgtggcg gtgggctttg
     1861 agggcacggt ccctttcgtg cacggcagcc agggttgcgt tgcctactac cgctcgcact
     1921 tctcgcgcca cttcaaggag ccgacgtcct gtgtctcctc ctcgatgacg gaagacgcag
     1981 ccgtgttcgg cgggctcaac aacatgattg atggccttgc caacacctat tccatgtaca
     2041 agcccaagat gatcgccgtt tccaccacct gcatggcaga ggtgatcggc gacgacctca
     2101 acgccttcat caagaccgcg cgcgagaagg gctcgatccc ggccgaatat ccggtgccct
     2161 ttgcccatac gccggctttc gttggcagcc acatcaccgg ctacgacaat gccatgaagg
     2221 gcgtgctgga atatttctgg gatggccgcg ccggcacgat cgacaaggtg gcgcgcgtgc
     2281 cgaacggcaa gatcaacttc ctgggcggct ttgatggcta caccgtgggc aacctgcgcg
     2341 aggtgaagcg catcttcgag atgatgggcg tggaatacac catcctgggc gacagttccg
     2401 atgtgtggga cacgccgacc gatggcacct tccgcatgta tgatggcggc accacgctgg
     2461 aggatgcgga aaaggccgtg cacgccaggg caaccatctc gatgcaggaa ttctgcaccg
     2521 agaagaccct gccgatgatc gccgcccacg ggcaggagac ggtggccttc aaccacccca
     2581 tcggcatcga ggcgaccgac cgcttcatca tggaagtggc gcggctggcc ggcgttgcca
     2641 tccccgatgc gctggccagg gaacgcggcc ggctggttga tgccgtggcc gacagcaacg
     2701 cccatgtgca tggcaagaag ttcgccatct atggcgaccc ggatctttgc tatggcctgg
     2761 cgcaattcgt catggaactg ggcggcgagc cggttgtggt gctggccacc aatggcggca
     2821 agggctgggc gaagaagatg caggccctgt tcgacagctc gcccttcggg gctggctgca
     2881 aggcctatcc cggcaaggat ctgtggcaca tgcgcagcct gctgttcacc gagaagcccg
     2941 attatctgat cggcaacacc tatggcaaat atctggaacg cgacaccggc gtgccgctga
     3001 tccgcatcgg cttcccgatc ttcgatcgcc accacaagca ccgctatccg gtgtggggct
     3061 atcagggcgg gctgaacgtg ctggtgacca tcctcgaccg tatcttcgag catatggacg
     3121 ccaccaccaa cgtgccgtcg cagaccgact actcttacga catcatccgc tgaagcgcgg
     3181 gcgatagcgc gggatctcaa ccgggttcgt gaggagaaaa gcgatgaaca gtctcagcca
     3241 gaccatccag gatgtcttca acgaacccgg ttgcgacaag aaccaggcca aatccgaaaa
     3301 ggagcgcaag aagggctgca ccaagcagct gcagcccggc ggcgcggcgg gtggctgcgc
     3361 tttcgatggc gccaagatcg cgctgcagcc gatcaccgat gtggcgcatc tggtgcatgg
     3421 gcccatcgcc tgcgagggat cgagctggga caatcgcggg gcgaaatcct cgggcccgca
     3481 gctctatcgc accggcttta ccaccgacat gggcgagaat gacatcgtct ttggcgggga
     3541 gaagcggctg ttcagggcga tccgcgagat ccgcgacaaa tatgccccgc cggcgatctt
     3601 cgtctaccag acctgcgtgc cggcgatgat cggcgatgac atcgaggcgg tgtgcaaggc
     3661 cgcttcggcc aaattcggca cgccctgcat cccgatcctc tcgcccggct ttgtggggcc
     3721 caagaacctg ggcaacaagc tggccggcga ggcgctgctg aagcatgtga tcggcaccga
     3781 agagccggaa tttaccaccc cttacgacat caacatcatc ggcgaatata atctggccgg
     3841 cgagctgtgg caggtgaagc cgctgctcga tgagctgggc atccgcatcc tgtcctgcat
     3901 ttcgggcgat gcgcgctatc gcgaggtggc ctggagccac cgggcgaaag cggcgatgat
     3961 ggtgtgctcc aaggcgatga tcaatgtcgc ccgcaagatg gaggagcgct atgccatccc
     4021 cttcttcgag ggcagcttct atggcatcgg cgacatgtcc gaaagcctgc gccagatcgc
     4081 cacgctgctg atcgcgcagg gcgccgatgc ggaactgatg gcgcgcacgg aagcggtgat
     4141 cgcgcgtgag gagcagcgcg cctgggcagc gatggaaccc tatgcccgcc gccttgccgg
     4201 caagcgcgtg ctgctgatca ccggcggcgt gaagagctgg tcggtggtgg ccgcgctgca
     4261 ggaagcgggg atggaggtgg tgggcacctc ggtcaagaaa tccaccgatg aggacaagga
     4321 aaagatcagg cagatcatgg gcaaggatgc ccatatgatc gacgacatga ccccgcgcga
     4381 gatgttccgc atgctgaagg cggccgaggc ggatatcatg ctgtcgggcg ggcgcagcca
     4441 gtttgtggca ctgaaggcca ccatgccctg gatggatatc aaccaggaac gccacgccgc
     4501 ctttgccggc tatcacggca tggtggagat ggtgaaggag atcgaccgca cccttgccaa
     4561 cccggtgtgg gaacaggtgc gcctgccggc cccctggcat gcggagccag gctggcagga
     4621 tctggcgctg gcggaaaatc cggcgccggt ggccgaaacg ccagccccgg tggccggcgc
     4681 tgcgcccgcg ccagacccgc gcgcgcgcgc cgtgtgccat tgcaaggcgg tgagcgtggg
     4741 gatgatcgcc gatgcggtgg cgctgggcca taccacgcgt gaggcggtgg ccagggccac
     4801 gcgcgccagc accggctgcg gcggctgcgg cccgtgggtg gacaaggcca tccgcgaggc
     4861 gctcccttct gccagcgcgg cggcctgagc catggcgcgg gtggaaaaat cggccaaggc
     4921 ctgcaccgtc aacccgctgc gcatgagcca gccgattggc ggcgcgctgg ctttcatggg
     4981 catggaaggc acgatgccgc tgctgcatgg cagccagggg tgcacctctt tcgggctggt
     5041 gctgttcgtg cgccatttcc gcgaggcgat ccccttgcag accaccgcga tgagcgaggt
     5101 ggcaacggtg ctgggcggtt atgacaatgt cgagcaggcg gtggccaata tcgccgcgcg
     5161 cacaaagccg gcgctgatcg gcatctgctc caccggcgtc accgagatca agggcgatga
     5221 tctggccggc tttgtgcagc agatccgcgc gcgccggccg gatctggccg atgtggcgct
     5281 ggtgcactgt tccacgcccg attttgccgg cgcctttcag gatggctggg ccagggccgt
     5341 gacggcgatg gtggagcaac tggtcgagcc ggtggcgcca aacgcgcggg cgcgcgaggc
     5401 ggggcgggtg aacgtgctgc ccggctgcca cctgacgccg ggcgatatcg acgccctgcg
     5461 cgacatcttc accgattttg gcctgacgcc cactttcctg ccggatcttt ccggcgcgct
     5521 cgatggccat atcccgcagg atttcacgcc gacaacgctg ggcggcacat cgctggcggc
     5581 cgtgcgcgcc atggggcagg cggcccacac cattgccatc ggcgcgcaga tgggcccggc
     5641 cgccgccgcg ctgagcgcgc gaaccggcgt gccccaccgc ctgttcgacc ggctgaccgg
     5701 ccttgccccc tgcgatgcgt tcatcgccca tctggcgcgg atcagcggcc ggccggtgcc
     5761 tgccaaatat cgccgccagc gcggccagtt gatcgatgcc atgctggatg gccatttcca
     5821 catcggcggc cggcgcatcg cgctgggggc agagccggac ctgctctttg cgctgggcag
     5881 catgctggcg gaaatgggcg ggcagatcgt ggccgcggtg acaaccaccg ccagcccgct
     5941 caacgcccgc ctgccggccg aacgggtgac gatcggcgat ctggaggatc tggaggcaca
     6001 ggcgcgtgac ggcgatgccc agatcctcat cacccacagc catggccggc aggcggccgc
     6061 gcggctggtc ctgccccatt tccgcatcgg cctgccgctg ttcgacacgc tgggcgccgg
     6121 gcatctggtc agcgtgggct acaagggcac gcgcgatctt gtcttcgcca tcggcaacat
     6181 gctgatgcac agccaccacg aggccgcgcc cgaggattgg cgcgatgcct ggaacgcgca
     6241 tccacacacc ccccatcgca aggaggcagc gcaatgaagg tagcctttgc cacgcaggat
     6301 ctgcagcgga tcgatgccca tttcggctgg gccaagaacc tggtcatcta tgatctgacc
     6361 cccgagggct ggacgctcga ccgggtggaa agcttcgagg gcgacctcaa ggaggatggc
     6421 gacgaggaca agctggcgcc caagctggcc gcgctgcagg gggtggccat tctggtggtg
     6481 gcggccattg gcggatcggg cgcggcgcgg gtggtggcgc agaacatcca cccgatgaaa
     6541 gtggccgagc cagagcccat cgaggccatg ctggagcgcc tgcgcgaggt gttgcggggc
     6601 acgccgccgc cctggctgcg caaggtgatg gcccgcaccg accgcgattt cgactttgag
     6661 gaagaggagg catgagcatg gccgacagcc aaaccctgca cgcaaccggg ggggcggccc
     6721 cggcagagcc tgccatcacc ggctttgccg cggatctggt gcgccagctg cgcgccaccg
     6781 atacccatgg cgcctgggat cgccgctcgg actggtcgct ccttgagccc ttcatcgtcg
     6841 acagggagaa gcgccgggcg atcccgatca tcggcgatcc cgatcccgac acgatctggc
     6901 ggctggagct gttctatggc gcgctgtgcc tcgccatcga gaagcgctgc ggcaagatgg
     6961 tcagcccgct gatgaagctc agccacgaag ggttcgggcg gatggtgctg gccgcggggc
     7021 ggctgatcgt catcaacaag agcctgcgcg atgtgcaccg cttcggcttc gacagcctcg
     7081 aaaagctgaa tggcgaggcg gaaaagctga tcggccaggc gctggagatg atcgcccggt
     7141 tcccggccgt ggcggattat tgaaggaggc ggcgatggcg gatgcagaag cgttgaaggc
     7201 cgaggtgaag aaactgtcgg cccaggcgat gcaggcgaag atggacttgc acgacctgtc
     7261 cgaggaactg cccgtggggg tggaaaaggt gccgcaagtg gcgcagcggg tggtggcggc
     7321 ctatgccgcg ctggccgagc gcaaggcggc gctgcaggcg ctggcagaaa cggcggcagg
     7381 aacggcggca tgagtgggct gctgacccgc ggcggccagc cctgggcgcc tgcctatctg
     7441 ctggcgatcg atcctgcgct gtgcatcggc tgcgggcgtt gcttcaaggt gtgcggccgg
     7501 ggcgtgatgg gcctgcatgg gctgaccgaa gagggcgcgc tggtggcgga tgacgaggac
     7561 gacgacgaga tcgtcaagcg ggtgatggcg ctggatgatg ccggcgcctg catcggctgc
     7621 ggggcctgcg cccgggtgtg ccccaccaat tgccagacgc atggggccgc cggatgatgg
     7681 cacaggggcc ggcgctttat gccgcgctgg tggcggccgg gcaggcggcg ggggccgatc
     7741 cgttcgattg ccatgtggcc gccgccattc tggccatggc cgccggcgag gaagcgggcg
     7801 cgccggcaga gggctgcggc agcgggctgg atgcgggcga gcgggcggaa ctgtcggccc
     7861 tgctgtttgg cggcgcggcg ctggcgggcc cggacccttg cgccatgccc cagcaggagc
     7921 agaccctgcg cgatatcctc tggatgaacg cgggcagcgg atcgcggttg cagcggctgt
     7981 tggcgcggat gatagcccgg cgggcgctgc ggcccaacca tttgtggcag gatctgggcc
     8041 tggcccatcg cggcgagctg agcgcgctga tgcagcgcca tttccctcgc ctggccgccc
     8101 gcaacgcggc cgatatgaaa tggaagaagt tcttctatcg catgatgtgc aacgatgccg
     8161 gctttgccct gtgcgccgcc cctgtctgca gcgaatgcga tgatttcgac gcctgtttcg
     8221 gcggcgagga gggcgaggcg ctgctggcgc gcctggccaa tggccggggg ctgcccccgg
     8281 ctgccctgcc agcccccagc aacggagccc atgcatgagc gagccggtct gttcctctcc
     8341 gcccgatgcc accccggcgc cgccggccga accgcccttc gatgcggccg gcgaggcggc
     8401 gatcatggcg atggtgcgcc gcttctacga tttgtgcctg gccgatgatc tgctgggccc
     8461 gatgttccgc gcggtgatcg atgatttcga cgcgcattat ggcatcgtcg ccgatttctg
     8521 gagccatgcg ctgctgggca cgcaccgcta tcagcgcggc acgccctatt cgcaccacac
     8581 ccatctcacc gtcagcgagg cgcatttcac gcgctggatg gccgccttcg ggcaggcggt
     8641 gggcgagatc ctgccggcgc cgctggccga caaggcgatg cagcgcgccg cccatatgac
     8701 cgccagcttc cgcatgggcc tgctgcccct gccggaaccg ggcccgcggc catgaatgcg
     8761 ccggccccgg cgcggcggcc ggcggtctat gccgtctgcg cgctggagga gatccccaac
     8821 cggcgcgcgc gcgctttcgt gctggcgcgt gccggggccg atggcgcgcc ccggccctgg
     8881 ccgatcttca tcctgcgctg ggggcgccat gtgcgcgcct atgagaaccg ctgcccgcat
     8941 cagggcgtgc atctcgactg ggagcgcggc gagttcctcg atggcgaggg catccgcatc
     9001 cagtgcggca agcatggcgc gctgttcgat ctgggcacgg gggcctgtgt ggcagggccg
     9061 tgcctgggcg aggggctggt gccgatcgag gcggtgatcg aggagggcga gatctgcctt
     9121 gccggcgtcg atctgctgga agacgaggac gaggacagcg ggcagggcgc cgaataggcc
     9181 cgcccgcggc caggcaaggg agcatgccat gccgcttttc gatattgcct gccccgcttg
     9241 cggggccacc agcgaagtgc tggcccgcgc ggccgatccg gtggtctgcc cggcttgcgg
     9301 cagccctgag gcgcgccggc tggtggcgca ggtggcagcg cccggcacat cgcgcgccct
     9361 tgccgccgcc gggcgggagg ccgcgcgcaa ggccggccat ctcagccact tcaagggcgg
     9421 ctgaccgggg cccccttgcc ggcatggctt tgcggccagg ccggcgcggg ggcagggctc
     9481 acaccggccg gttctcgttg cggttgcgca ccggcgccat gctggccacg ccttcctcat
     9541 aactcagttc gggatagggg gccttgaagg cggccacctt gtcgagcaca tgggcgagct
     9601 tctgcgcgcc cgacatcttc ttcacatcgt tcttgtcgac atcgacgagt tcgagcgttt
     9661 ccttccacac ggtcgattca tcgagcggca gcacataggc cgttgcaccg ctgccgccca
     9721 gcggcacgcg gtatttcttc aggaaatcga tgttgttggc aaagagcaca tagctgcccg
     9781 ccggatcgag gcaatcggcc agcacttcgg cgacatcctt cagccaggcg aaattgtgga
     9841 gatagcccac cagcaccggg atcttgccgg tggccgcatc cgcgcgcgcc agcacctgtt
     9901 ccagcgcata ggccggcggg cgggcctcgt cggccacctg atcctggaag gcaagggcga
     9961 tatccccgcg aaagccgaaa gtgccggccg ccggcagcgg ctccttgagc acggcgttga
    10021 gcaggcggtg ggcggcattc agccggccga gggcagtgtc ctccagccgc acggcatcca
    10081 tggtcgacat cggtttcgtc tccttggcga aaggggcgca ggcccgggcc aaccgttggc
    10141 ccgccgcgct ggcccgtgcc atgcacgatc cgggccgttt gccgcgcaca ggcccggggc
    10201 gccgggcagc cgcgcccgcc cgcccggctt tgtgggcatt ggaacaggcg cgtttgtcgc
    10261 aaacccgcca aagcggacag ggcctcccgc ccgccggccg gcatggatgg ggcacaaggc
    10321 gcgcccgccc ccgcgttttg gccatgcggc cgtttttggc acaggccttg ctgcacatcg
    10381 gggcataaca accgatgcgg ggacaaggca catgatcttc ctcacagaca gcgcggccca
    10441 ggctgtgcgc acggccatcg gccaggcggc cagcgcgccg gccgggctca ggatccaggt
    10501 ggaggcgggc ggctgcgccg gctacaaata catgatgggg ctggtggccg agccgcggcc
    10561 cgatgatgcc gtggtgaagg agggcgatgt cgccgtcttc atcgatccgg ccagccagcc
    10621 gttgatcgcc ggcaccacca tcgatttcgt cgtcgccctc gaaggatcgg gtttcacctt
    10681 cgagaacccc aacgccacca acagctgctc ttgcggcaag tctttcggct gaaggagcca
    10741 cgcgcatgtg ggaatatagc gagaaggtga aggactactt cttcaacccc aagaacagcg
    10801 gcatccttga agatgccgat ggcgtgggcg atgtgggggc gatcagctgc ggcgatgcgc
    10861 tgcgcctgat gatcaaggtg gatgcgccca gccagaccat caccgacgcc cgcttccaga
    10921 cctttggttg cggctcggcg atcgccagca gctcggcgct gaccgagctg atcatcggca
    10981 agaccatcga tgaggcgatg gggctgacca accaggacat cgccgatttc cttggcggcc
    11041 tgccgccgga gaagatgcat tgcagcgtga tggggcagga ggcgctgcag gcggcgatcg
    11101 ccaattatcg cggcgaggcc tgggtggacg atcatgagga aggcgcgctg atctgcaagt
    11161 gcttcggcgt ggacgagggc atgatcgagc gcaccgtgcg caccaatggc ctgaccagca
    11221 tcgaggagat caccaattac accaaggcgg gcggcggctg ttccacctgc gccgagggga
    11281 tcgagggcgt gctcgagcgg gtgaatgcgg cgatggtggc agaaggcctg ctggccgccg
    11341 acagggcttt ccacgccggc gtggtgcccc tgccggtgaa ggcgcgcaag gtggtgcgca
    11401 ccgaggcgcc ggcggcaagg gcgctgtcca acctccagaa gatccgcctg atcgaggaga
    11461 ccatcgcgca gatccgcccc agcctccagc gcgatggcgg cgattgcgaa ctgatcgatg
    11521 tggaaggcaa ccgcgtgctg gtgcggctga cgggggcctg cgtgggctgc cacctgtctg
    11581 gcgccaccat cgagggggtg caggcgcggc tggtcgaggc ggtgggtgtg ccgctgctgg
    11641 tggtgccggt ttcggcgatg cattgaaagg agagcggcca tgagcgccca acctgtctat
    11701 ctggacaata acgccaccac ccgcaccgat ccggcggtgg tgacagcgat gctgcccttc
    11761 ttcaccgaac agttcggcaa tgcctcctcg atgcatgcct ttggcgccga ggtgggcggg
    11821 gcgatcaccc atgcccgccg ggcgctggcc gatctgctgg gcgccgcatt cgaccatgag
    11881 atcgtctata ccggcggtgg caccgaggcc gacagcaccg ccatcctgtc cgcgctggaa
    11941 acccagggcc agcgcaacga ggtgatcacc agcgcggtgg aacacccggc cgtgctcagc
    12001 ctggtggccc atctggaaaa gaccgggcgc gccagggtgc acatcatccc tgtggatgcg
    12061 cgcgggcggc tggatatgga agcctatgcc aaagccctgt cggagcggac cgcgatcgtc
    12121 tcgatcatgt gggccaacaa tgaaaccggc acgctgttcc cggtggagcg gctggccgag
    12181 atggcccatg gcgcaggcgc cctcttccac accgatgccg tgcaggcggt gggcaagatc
    12241 cccatcgacc ttaaatccac cgcgatcgac atgctctcgc tgtcgggcca caagctgcat
    12301 gggcccaagg gcattggcgc gctgtatgtg aggaaggggg tgcgcctgcg cccgctgatc
    12361 cgcggcggcc atcaggagcg cgggcggcgc gccggcaccg agaatgcgcc ggcgatcatc
    12421 ggccttggca aggcggcaga gctggccgcg gcccacatgg ccgaggagca gacccgcgtg
    12481 aaggccctgc gcgaccggct ggaggcgggc ctcctggccg ccatcggcaa cagtttcgtg
    12541 accggcgatg tggagagccg cctgcccaac acctgcaaca tcgctttcga atatatcgag
    12601 ggcgaggcga tcctgctgct gatgaaccgc gaggggatcg ccgcatcctc cggctctgcc
    12661 tgcacgtcgg gcagcctgga gcccagccat gtgctgcggg cgatgaacgt gccctacacc
    12721 gccgcccatg gcgccatccg cttcagcttc agccgcgaga acggcccgga ggatgtggcg
    12781 cgcgtgctgg aggccatgcc gccgatcatc gccaggctgc gcgagatgag ccccttctgg
    12841 cgcgagcgcg accagcacac cgccgccttc aaccccacct atgcctgaca cctgctgaaa
    12901 ggagccggcc atgccctgcc aagccccatc ggtgatcatc aacgacagca ccctgcgcga
    12961 tggcgagcag gcgcccggca tcgctttcac tccggccgag aagctggcca tcgccctggc
    13021 gctggatgcg gccggggtgg acgagatcga ggccggcacg ccggcgatgg gcgaggcaga
    13081 gctggccgcc atccgcgata tcgccagcca ggcccggcgc gccgcggtga tcccctgggg
    13141 ccggttgacg cgggccgatg cggatctggc cgcgcgggtg ggggccgggc gggtgcatct
    13201 ctcggtgccg gtgtccgatc ggcagatccg tgccaaattc ggccggcatg gcgggggcgg
    13261 gcggctggat gtgctggcgc gcattgccga tgtggtgccc tatgcgctgg acaggggcat
    13321 gcgcgtgtcg gtgggcggcg aggatgccag ccgtgccgat cccgatttcc tcgcccgcgt
    13381 ggtggtggcg atcgaggagg ccggcggcca ccgcttccgc tatgccgaca cgctgggggt
    13441 gctcgatccg tttcgcgtgt gcgatgcctt tgccggcctt gcccgcgaaa ccgatatcga
    13501 gctggaattt cacggccatg acgatctggg cctggccacg gccaacacgc tggcagcggt
    13561 gcgcggcggg gccagccatg ccagcgtgtg cgtgctgggg ctgggggaac gggccggcaa
    13621 cgcgccgctg gaggaagtgg tgacggcgct gggcgagacg ctggggcggc gcacgggggt
    13681 ggatccggcg cggctgggcg ggcttgccga actggtagcc agggccgcct gccggcccat
    13741 ccccgataac aagccgatcg tgggggccag cgtgttcgcg catgaatcgg gcatccatgt
    13801 ctcgggcctg ctgcgcgatg ccggcactta cgaggcgctg gcgcccgaac ggtttggccg
    13861 gcgccggcag atcgtgctgg gcaagcattc gggccgcagc gcggtacgca gcgtgctcca
    13921 caccattggc cttgatgccg agcacgagcg gctggaggcc attctggccg ccgtgcgtgc
    13981 gcgggcggtg gccaccaagc ggccggtggg cctggccgat ctggccgccc tgtatgagca
    14041 gaccgcgtga gggcgcccgg ccttgtggcg ctgatcgccg gcgatatcgc ctgcgtgcgg
    14101 gcgcgcgatc cggcggccaa cagccgcatc gagatcgcgc tgacctatcc gggcgtgcat
    14161 gcgatcatct ggcaccggct ggcgcatcgg ctgtggcggc gggggtggcg gtttgccgcg
    14221 cggttcctcg gctggctggc gcggctgctc accaatgtcg acattcaccc tgccgccacc
    14281 atcggccagc gctttttcat cgatcatggc gccggggtgg tgatcggcga gacggccgag
    14341 atcggcgatg atgtgacgct ttatcatggc gtcacgctgg gcggcaccag ctggcagggc
    14401 ggcaagcgcc accccacgct gggcgatggc gtgctggtgg gctgcggcgc caagatcctg
    14461 gggccgatcg gcgtgggcgc cggcgcgcgg gtgggggcga acagcgttgt catcgaggat
    14521 gtgccgccgg gcatgacggt ggtgggcatc cccggccggg tggtgcgcgc cccgcgtgag
    14581 cggcggcgga tcggcgagcg gatcgacctt gaccaccatc tgatgcccga tccggtgggc
    14641 acggcgattg ccgagctgat cgaccgcatc gattttctgg aggcgcggct ggcgcaccgg
    14701 gacagggccc tgcctgtccg ttccaggccc gcgcattccg ccgcaacggg ggagaattgc
    14761 catgagcctg cttgatcgcc tgcaggcgct gtccagcgcc gaggatttct tcgccctgct
    14821 cgatgtgccc tatgacgagg cggtggtgcg cgtggcgcgg ctgcatatcc tgcggcgcat
    14881 gggccagtat ctcaggggat cggaggccga gggcgccttc gacgggctgg acgaggtggc
    14941 cctgcagggc ctgtgccgcg cccatctggc gcaggcctat gccgatttcg ttgcatccag
    15001 cccgatcgcc gagcggatct tcaaggtcca tcaggaagcg atcgctccca aacccgagcc
    15061 caaccggcct tttgtgccgc tcaccgcgct taccggaggc atagcatgac cgacccgctg
    15121 catatcgtcg tgtgcatcaa gcaggtgccc gacagcgccc agatccgcgt gcacccggtg
    15181 accaacacga tcatgcgcca gggcgtgccg acgatcatca acccctatga tctcttcgcg
    15241 ctggaagagg cgctgcggct gcgcgaccgg ctgggcgggg tggtgacggt gctgtgcatg
    15301 gggccgatga cggccgagga ttcgctgcgc aaggcgctca ctttcggggc ggaccgggcg
    15361 gtgctgctgt ccgatcgcta tttcgccgga tcggacacgc tggccacatc ctatgcgctg
    15421 gcgatggcgc tggcgcagat cggccgggaa tttggcccgc cgcagatcat cttcaccggc
    15481 aagcagacga tcgatggcga caccgcccag gtgggcccgg gcatcgcccg ccggctgggc
    15541 ttcaaccagc tgacctatat ctccaggatc cgcgaggtgg acccggcggg ggggcgcatc
    15601 tgcgtggagc ggcgcgccga gggcggcgtg caggtgctgg aaacgcgcct gccggtgctg
    15661 atcaccatgc tggaggctac caacgagatg cgccgcggcg cgatcgagac ggcgctggcc
    15721 gccgccaggg cgcaggtgac ggtgtggaac gcggcctctg tgggcataga ggatctgacg
    15781 aaatgcggcc tgcgcggctc gcccaccatc gtcaagaagg tgtttgcccc cggcgcccgg
    15841 gccgaaaagg ccgcccagat cgccctcggc gacacgcccg aggccaccgc ccacgccgct
    15901 atcgaggcga tcttcgcgcg cctgcccggg ctggaggcac aattgttcga aaccgcagcg
    15961 gccagctgct gacacaggat cgcaaggagg c
//
LOCUS       DASRYE010000322        27151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_66775,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000322 DASRYE010000000
VERSION     DASRYE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 27151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27151
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66775"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..2290)
                     /locus_tag="VFF98_16690"
     CDS             complement(185..2290)
                     /locus_tag="VFF98_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65328.1"
                     /translation="MKTTPLALALIAGVSPPALAQPGAMALPPVDIDGSSDAAATLTP
                     ADLGAAAPAASDTAALLARLPGVAGNSGGGFSTMPVIRGLSEQRLTITVDGHPIEAAC
                     PNDMNPPLSYTNPQTVGTIAVITGIPPVSAGGDAIGGSIAVKSAAPRFATTGRLLVTG
                     EAQGYFRSNDGGFGTGLTLTVAGARLSATYTGSYTRAGNYSAGGDLGTVHASQYAKTD
                     HQLALAYQGDIGLVELAGGYHHSAYEGFPNQFMDLTGNNSWFLNGHYLGLFGWGTVDL
                     KADYRATDHQMNFLADKGGSADGGMPMNTRVRAGGYTLAVELPVAPGTRLRLGQEYRN
                     ERLDDWWPPVAGSMMMGPDTYLNVNHGQRERIAGYIEAEMHRGDRFTLTAGGRFDRVA
                     MNTGDVAPYSTGMMSMADAAAASAFNAASHQRHFNDWSGALTGHYTPAPGLALDLGYA
                     HKARAPNLYELYTWGQGAMSSQMIGWFGDGNGYFGNLALKPERADTISGSITFSGRGK
                     QGWFVKLAPYYTHVNDYIDARYVKSLGAFAQLQFANEEAQFYGLDVSGAATVWNGGRF
                     GQTRFSGTLSWLRGQNLTDHTPLYHQMPLNLMASLEHTLGGFTGTLELNWVDSKTRVD
                     TARNEPTTPAYALVNLRGAYQRGSWRLSLAIENLLDKAYYLPLGGLALGDYSASGGAV
                     LRPVAGRGRSVNVGLARRF"
     gene            complement(2390..2776)
                     /locus_tag="VFF98_16695"
     CDS             complement(2390..2776)
                     /locus_tag="VFF98_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HZU65329.1"
                     /translation="MRILIRTRRWLAITLIGLALAVRALLPQGYMPAAGPHVLSVQIC
                     ADASGLPHLRQITLPARPPAHNGDHHQACAFAGHAMPLLGGADPLLLWAALLLAMAAA
                     LAPVAAALPARPARLRPPLRAPPLLS"
     gene            2882..3463
                     /locus_tag="VFF98_16700"
     CDS             2882..3463
                     /locus_tag="VFF98_16700"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZU65330.1"
                     /translation="MNSDRLKALTDGVVAVIITIMVLEMKPPPSAAPAALLALAPVLL
                     SYVLSFVYVAIYWNNHHHFFALTPRVSAGIMWANFNLLFWLSLIPFATAWMGEHELQA
                     WPTAVYGGVLLACALSWYLMQSVIIRAQGPQSDLRRAIGRDWKGKFSPPLYAAGMALS
                     FWRPALAALIYAFVALLWLVPDSRVHQAASRGA"
     gene            3572..3910
                     /locus_tag="VFF98_16705"
     CDS             3572..3910
                     /locus_tag="VFF98_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZU65331.1"
                     /translation="MIFGLGKTARHQEIEARALAAMVAADEVVVVDVREADEFAAGHI
                     PGALNMPLSAFQPGALPAHAGKALVLNCQGGKRSGMALDRCAAAQAAADTHLAGGFAA
                     WVAAGLPVAR"
     gene            complement(3942..5147)
                     /locus_tag="VFF98_16710"
     CDS             complement(3942..5147)
                     /locus_tag="VFF98_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU65332.1"
                     /translation="MTDTLPPPALAANRIVDFDIFNPPGVEEDYFAAWQSLQGPGKPA
                     LVWTSANGGHWIATDGALIRALWEDHARFSSACLAVTPGLGEVMAFIPLQLDPPEHKP
                     YRTAVMKGMASRYITALEPYVEASARDLAEALAPRGACEFVSDFAEIMPINIFLSMID
                     VPIEDRPQLRELGLQLTRPDGSVTVEQLKQAADDYLAPYVEARLARPGSDLFSRILAE
                     PIDGRPWQLDEARRLCRNLLFGGLDTVAAMLGMVALHLARHPEDQRRLRAQPALIPDA
                     ADELIRRYPTVSVSRNALVDAEVDGVRVRRGDIVYLPSVLHNLDPACFEQPEQVDFER
                     RLTPVRHTTMGAGVHRCVGAALARREVIAFLQAWLAAVPEFRLDPARPVRMKGGNVAA
                     ATALPLRWD"
     gene            complement(5283..6353)
                     /gene="arsB"
                     /locus_tag="VFF98_16715"
     CDS             complement(5283..6353)
                     /gene="arsB"
                     /locus_tag="VFF98_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HZU65333.1"
                     /translation="MPDTPARQRLSFLDRYLTLWIFLAMALGVGLGASFAGLPAAMGR
                     LSWGGTNIPIAIGLILMMYPPLARVRYEELPHILDDRRILLLSLVQNWIIGPVLMFAL
                     AVLFLADRPEYMTGLILIGLARCIAMVIVWNQLARGDSQYVAALVAFNALFQILFFSL
                     YAWFFLAVLPPLFGLEGSVVHVSFQTIAQAVLVYLGVPFLAGFCTRKWLRAARGADWY
                     EHRFLPAIGPVTLVALLFTIVAMFSLKGSEILTLPGDALRIAIPLTIYFLLQFTISFA
                     MGRLIGSDYPRTTAVAFTAAGNNFELAIAVAIAAFGLASPVAFAAVIGPLVEVPVLIL
                     LVNLAFWLGRRWFPASVPARQG"
     gene            complement(6392..6823)
                     /gene="arsC"
                     /locus_tag="VFF98_16720"
     CDS             complement(6392..6823)
                     /gene="arsC"
                     /locus_tag="VFF98_16720"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542918.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HZU65334.1"
                     /translation="MHTDIIIYHNPACGTSRNALAMIRNAGIEPHVVEYLKTPPARAM
                     LESLIARAQISPRALLREKGTPFAELGLDNPDLTDSQLIDAMMDHPILINRPLVVSPL
                     GVKLCRPSETVLDLLPAAQRGAFAKEDGDQVIDAAGRRVGS"
     gene            complement(6845..7189)
                     /locus_tag="VFF98_16725"
     CDS             complement(6845..7189)
                     /locus_tag="VFF98_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU65335.1"
                     /translation="MEEINLPVWAVDALGALAHETRLAVFRMLVQAGPTGHVAGAIAE
                     RAGVPPSTMSHHLATLERAGLVHSERESRLIRYRADYAGMRRLLAFLMQDCCQGAPEM
                     CADLGAALSCAG"
     gene            7387..7908
                     /locus_tag="VFF98_16730"
     CDS             7387..7908
                     /locus_tag="VFF98_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65336.1"
                     /translation="MIRHVMTAAAFVGALAASAAHADAPAKNWSAPAYRIYAQQLSDE
                     TMQANPDLLSVTFHGIPPGAVGETFTMYAGSYPDRIGNPDDPDDIDVSKKGITIVDPR
                     WHRTKDPAPKFVVLMPLRDASMNNIGLIVYAFKVPVESNAGERAFYARALDLRDALAR
                     QIPSYEALFAPAK"
     gene            8113..9438
                     /gene="dctA"
                     /locus_tag="VFF98_16735"
     CDS             8113..9438
                     /gene="dctA"
                     /locus_tag="VFF98_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HZU65337.1"
                     /translation="MIGGDVEGGAQARRAPHHSLYIQILLAILAGVVLGVLAPDVAVQ
                     CKVLGDMFVRLIRMVISPVIFLTVCTGIAGMRAPGALGRVMAKAMAYFLALSTLALVI
                     GLAVALFVQPGRGMNVDIGALDGQSVADYVVKAHAQSAQGFLLEIIPDSLVSPLAGTN
                     VLQALVAAVLIGLGVSFIGARAEPLRALLDQAALVVFRVVELVMRLAPVGTFGAMAFT
                     IGKFGAGTIARLAGLVGCFYLTSALFVTVVLGSVALLNGFSLWRLLRYLRDELLLVLG
                     TSSSEAALPSLMGKLEEAGCDKSVVGLVVPAGYSFNLDGTNIYLTLAALFLAQACNVH
                     LGLGQMALLLGVAVLSSKGAAGVTGAGFITLAATLAIVPSVPVAAMALILGVDRFMSQ
                     CRSLTNFIGNAVATIVIARWEGGLERAALDRALGGASILTDAGQFGIAE"
     gene            9474..11789
                     /locus_tag="VFF98_16740"
     CDS             9474..11789
                     /locus_tag="VFF98_16740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65338.1"
                     /translation="MISQNTLALALMLASTAPVAAFAQEAPPPERHGQASASAKGHEA
                     PTNEEIIVTGRFIDTGAKSAMKMPVSVLDTAATVSSYSSSFVKSLDTTTVSDLYNYMT
                     GLKKSGNTGYDITLRGFKGSGDDRNTILVDGLPGLTVRYGSPGTVNIDHIELVQGPTS
                     VLYGQAQPGGFVNLVTKSPLGHFEASVEQRVNSYAGSGLPLGRRNGIEDEFDVTGPIT
                     RSGNLLFRVTGQISDIQSFRDGALASSSEFIAPALTWKINPGTSLTVKAEYGHVRQHL
                     DNGVVAPANGNIYDVSRAAPRTASYQQPDDFRGETGKTVSAFLHHDFGGGWQFHASYR
                     HVSYNSDQKSFDQYGTLTVQGVAEVQRRARELVTNRSYDYGDINLNGHFKTGFIKHKI
                     LIGLNLGSNSTENNRIKFFNSSTPDSTTGKCPTGGTCLNIPVYNPSAYYSSFPSFDSL
                     PAINTQLGAAAQKALLTDQLSKTRSYGIYVSDLMELAPWLKVSIAGRNFAETSKVAPD
                     LRNLPGVSYKRTDRRNLLPSAGVLIEPTRHMTVYASYSESFVPSDPSALDGNGNVGTL
                     NPVTAKQYELGLKGENLFQRRLSFTAALYRIDQDGQVTTNPCIYGTCSYQTGKGRSDG
                     FEVQGDASPFENLQLLFGYSHIIAKIRTSPNLPFQVGDVLPNVARDAANLWSRYNVGH
                     GLGLGLGIVYTGPRQGVLPTSASDTKLMPLPGYVVVDTGIYYDINRWSINLKIGNLFD
                     KTYIESAGATGRIQAAYGQPRNLTLSVKVKL"
     gene            11786..12931
                     /locus_tag="VFF98_16745"
     CDS             11786..12931
                     /locus_tag="VFF98_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65339.1"
                     /translation="MRQQGSSGRIPQGWQVAALVAAALPVAAPAQDMPQYVPQAQVSG
                     EIRDYGFGLGGVLAQWEAAFRAFQPHVTFKDTLPTSDAAFPGLVTYQADLGIDGGEPA
                     ITEALSFWETRGYAASSVTVASGAYDVEGKSNGPVVFVARDNPLNSLTVDQLDGIFGA
                     ARNGTLDGFVWSPQGARGADRDIRNWGQLGLRGAWAAKPIQTYGHGPSGTTRFFQWQV
                     LGNTDKWNPNYRGYVETGSKQIAAIDRAGQLLGARHMLGEELAHDRYGIGWSTMSQAK
                     GIAGLKILAIAPRGGGAAVMPSQATFADRSYPLVRNIYIYFDRQPGTAMDPKLREFLR
                     FVLSAQGQRLVGAAGYLPLPAAMVLRERTRLDGGVAPPAPAPGKDRP"
     gene            12931..14655
                     /locus_tag="VFF98_16750"
     CDS             12931..14655
                     /locus_tag="VFF98_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65340.1"
                     /translation="MSHHIAPWLALAALLAATPALAEPAHILITVPAQARAPADFATT
                     VANWRQAGEIADAIWLEADGREGGSFQTFVQLDFPGEAELKAWQASEGARLAAPLEAH
                     RVAILTHGETLPRDSNLSVFQVDSFQGTSAAGLADYAAHYVRPLMEGERAAKVLMRYT
                     VYTEHQPDGHDRAWTLSEYHDPAAFERLPAIAQNVSAALDRADPAFARLGHSLAAYRT
                     PEALTRARYVELPPPALAGLPDYKPEYHITGTLRIYGSELKNAVEYLARAFQSYQPDM
                     RVTWSNATSSEGAIAGLYTGVSDVAPMGDDAKLTDEMPFFNVNGYLPTEISVATGGYE
                     RRGSLWAWVIVVNKDNPLNEISVDELTHIFGAERSGGWEQANNNWLYTSKYAVDKAQN
                     LRSWGQIGVGGAYAGHEIQTYGFSAPGFQAAFERHWFHWSHKWNPNLMEYVERKEAVD
                     GAQGDAVASEQPLKIVSNDRYGMAIAGLLHVKDFPNLKILKISEHKGGPYIPFTPATV
                     ANRSYPLHRDAYFYVNKQPGQPLDPKVREFMRFVLSRQGQQIIARVGYYYPIDRAALE
                     EQLKKLDN"
     gene            14685..17081
                     /locus_tag="VFF98_16755"
     CDS             14685..17081
                     /locus_tag="VFF98_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65341.1"
                     /translation="MKRMQASGAMALIALAATMTSAQEENAPDSKRPTGETYDQILHR
                     LGDGMLENIVIRGEHNARTDARAHKRFYTDTAPLADLPPYRPGRQVSGTIRVSGLYLH
                     DGLIMDRWIRDFQRFQPGAKVIISQHGTIASGKVDIETGPRINDRLRAASEYERATGL
                     RMFEIDWATGSYNVPGWSPGFVIFVHKDNPIAHLSLAQLDGIFAGARTGGWQGTQWDP
                     KAARGPEKNIRTWGQLGLTGEWANKPIHIYGRPLKYNIQLGFERKVFHGGDVWNENTA
                     EYSHEMNPDHTRYTSSVEMVKDMAADPYGIVFSDMGSAMPAVKAVPIGATDKGPFVPI
                     SLQTLRDRSYPLFIEEWAEARLQPGKPLDPLVKEFLTFMLSREGQDAIQQDGKWLPLP
                     AARARAMRAKLDTIGPRADPRALGLQTDMIAPPKWEGEAPDETGRIDPRRAYYAPDFD
                     LSGLPAYVPSAPLSGTIRMPRSGQLMASTMGQALIAAFRRHQPGLAFALADGDLIDHA
                     VDLSIGRKWTSYFAGEFYDFQLKHDRSPREFEIATGAFDVPGWNPAIAILVNRANPIE
                     GLTIRQLDGIFGGPRRGGWVSTTWRRQAGREAEGNIRSWGQLGITGACASRPIDVYVP
                     PLKYHQMSVFERKVLVGGNMWNDSAREIPLALRKDMSRRVPSAERAAAVAADPCGITF
                     VSAGFARAGTRLVPIAAGAGQRFVAPTLASVQDRSYPLGLEAYAYADQLPGQPLDPGV
                     REFLRFVLSREGQEIIMRDGKWLPLTPALAAAQTARLDEIIPQVVAPKAAAHKGETPQ
                     "
     gene            17078..18319
                     /locus_tag="VFF98_16760"
     CDS             17078..18319
                     /locus_tag="VFF98_16760"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65342.1"
                     /translation="MNKPHVLAQYAVAGALACAVPAGPVWAQEAAPPAPAPAAAEAGA
                     ETNANRSIWTLNVTSFRKQHALEGGYTKQWDLSGLPHYHPTRQLTGTLRIWGSNYLKD
                     GPIGAYWRQAFAKFQPGITIEYNLPTAGIAIPALAAKVADLGVGRPATLMDYLTFQQV
                     YGFTQTEIEVATGSYDVYGWSPAYVIVVNKDNPLAQISMKQLDGVFGTARNGGYDHSV
                     WRTDYPFRRGAEENIRTWGQLGLTGEWANKPIHPCGQSAHANIMSVFSDKVLSGSDQW
                     VEGYRAFANYATPDQKIAAWTSQVRKAVEGDPLSLCVIAPNGLSAGMRELAVQGWGGG
                     PFVARSLESVRARSYPLTNEIYFYAINGKGHPIDPKAEEFLRFVLSQEGQQEIQHEGR
                     YLPLTAAMVQAQLAKLAPAGQ"
     gene            18316..19428
                     /locus_tag="VFF98_16765"
     CDS             18316..19428
                     /locus_tag="VFF98_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65343.1"
                     /translation="MSRASWRRAIARGLAGAALLAGVAPVARAQGAAGPGLDPAADPA
                     MSATLASLPPYQAGPRVAGTIRIWGHGSPTHDFVGDLVRRWFAGFHRSQPDVALDYRM
                     YGTASAVGALATGAATLAILGEEISPDAQRLFRRARGYAPTEVDVANGSRDANYFDYA
                     HMIFVNRANPLTGLTLRQLEAVFGAEHRCTRRNLRQWGDLGLKGDWARRPITPYAWKT
                     DVDFALFFRERVLCGSHRWNPATREFMPVTRPDGTVVQHGQLILEALAKDRYGIAISN
                     IRFANPAVRALPLAWRAKGGFTMADPASLIARTYPLGRIIPAYVDVPPGGHVGPAERE
                     FLRYILSREGQQALAEASGYLPLDRASQTRALAVLR"
     gene            19455..20294
                     /locus_tag="VFF98_16770"
     CDS             19455..20294
                     /locus_tag="VFF98_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65344.1"
                     /translation="MLASALLLAPALLPAPAGAELAPPQPMRPAGTLVIATSAALAPA
                     LRRAAHRFEAAHAGVHVLILGQGSDVAMARLYTGQADAAVIGRDASDNEIKAYAWIYG
                     HAPQAEALGAGSRATPGLSPALAVIVAARSPLRAMTLEDLRQALATAPGPFHIVMPDA
                     QSGTGRFLRHRLHQDAAQFYWPRVREIAPPAPGQAGRDGEIDRAIATAVAADPKALGF
                     AAAPRRPGLRVLPIRAGGRLLRPADRDYPLRRTIMAYRPVGAQPLLIAYLACLRGVFP
                     PAS"
     gene            20383..21420
                     /locus_tag="VFF98_16775"
     CDS             20383..21420
                     /locus_tag="VFF98_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65345.1"
                     /translation="MRQWLFAAAACACLPFAALADTHAAAGGSPLKIIGSGELPGYEG
                     DFDHFAVDTAGQRLFLAAEDHGTLEVFRLGSLAHLKTVKGPIETPHAILFLPDVHKLL
                     VTETGPSLPHYFNADTYAFEKPLKLVQGADSMGYDAPRNRAYIVTGGKEVPMPDSWLE
                     QVNPRTGARINKAHFASANVQGMAVEQAGPHLYINVSDQHYIAVLDKVSLKELARWPV
                     DPALGTNCCFAFDEPGHRLFLATRAPGNLLVLDSRSGAVLARFKAPERVDQIEWDARH
                     RRVYVIGGEGWIQIIEARGKDQFVELPRLDTAPGAKTSVLVPSRNELYVAVSPGDSKA
                     MARILRISTGR"
     gene            21422..22501
                     /locus_tag="VFF98_16780"
     CDS             21422..22501
                     /locus_tag="VFF98_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65346.1"
                     /translation="MRKLSMLLAASLAVAGTAAALHAEDKAPVPAGFFRLQSVARLPG
                     GAPDWDYLAYDSQRQRLFIDRRDDGLWVFDTRLGKVLRRMPHTEGAGATLLVPALRRG
                     FVTNEDGSTTVFDMASLAVIKRLKFASDADAASYDPVSGRVAFVSADSQQVTLVDPRG
                     LKVVGTLKVPTGKADGSAADGAGHILLNARDRAMVLQIDAASARVLAQWPVTGCSQPT
                     GMAIDTASHRAFIGCRGAQPVLAVMNTQTGQVVQTLDIGRGNDGVVYDPVHHRILTTN
                     GVDANIVAIHQDDADHYRIEQAVTTRPFARTMAYDPQRQAIFTVTAEGVVDPAQPVNT
                     GPARFYPNAYFPGSMVVLTYAPTRK"
     gene            22602..23951
                     /locus_tag="VFF98_16785"
     CDS             22602..23951
                     /locus_tag="VFF98_16785"
                     /inference="COORDINATES: protein motif:HMM:NF015355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HZU65347.1"
                     /translation="MNVITETPASEGLQSGAMAPALPAAPAGEGWAIGVVPWPVYLVL
                     VAVLAAFTVVGKVPGDISVMIAALAVGGFACAELGKRLPGLSQLGMAAIFATFVPSYL
                     SYSGLLPKLWVKAITTFTKDSQILYLYIAAIIVGSVLSMDREVLLRGFLRIFVPMAAA
                     TLAAVAVGLGVGMACGLSAHDVFFFVLVPALGGGVGEGAIPLSIGYAGLMHQEQGLLL
                     ARILPAVMFASLTAIVFSGLISAYARSHPELTGNGTLQVDEHDEIGLLNARPEEAVTP
                     AGIAGAGATAIALYLVGLLGQSLLGFPGPITMLILAILLNLGRVVTARLKAGSYAYYR
                     FFSVAVTYPLLFAIGVAMTPWDKLMSAFTPAMILTIVATVATLMGTGFVVGRLVGMFP
                     VEAAIVTVCRASQGGTGDVAILTASNRLQLMPFAQIATRIGGAATVTTALALMKLWG"
     gene            23991..24773
                     /locus_tag="VFF98_16790"
     CDS             23991..24773
                     /locus_tag="VFF98_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65348.1"
                     /translation="MNTQTLTGDLRQPLIGALAKVPQVTIGFWAIKILATTLGETGAD
                     LFTQQYAMGYAAGIALFCVPLVVLVAAQIMAASFNKWLYWATILASTTAGTALADFFD
                     RSLGIGYTGGAATLLACVLGSLLAWRLVLGTVSVASVATPRAETFYWITITFSQTLGT
                     ALGDWAADDGGLGYAGGAAVFGGALLVLALLYFLTGASRVALFWAAFILTRPLGATVG
                     DFLDKPLAQGGLDLGRDYATYVLIGVIAVALWFVPQRPGKAH"
     gene            complement(24858..25976)
                     /locus_tag="VFF98_16795"
     CDS             complement(24858..25976)
                     /locus_tag="VFF98_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65349.1"
                     /translation="MHTIPSPFRPARRRPARRALLAALAGSALCSLIGAATIARSAEP
                     LPAPYVPRALSVPADKDYTVGPDTVFVAGNDVLIPLFDKLDVLWAQKEPNIKFQKIMM
                     GSTLSVEGLTSGKSAFGPIGRGNRRAEVDGFTQRFGYPPTYIKIGYDSNPNNKLSTGV
                     WVNASNPIKQITLEQAAAVFTTGGKQGDITHWSQLGVGGDVGPVPIHPYVTRDTSAIL
                     AMIDEMREVFGGHPFTPQTEWMPTEESAINAVAQDAYGMVLMDYWNNTAPMNGVTTWK
                     HLADFGPRIKFVPMVIDDRGTLSDGTMEGPLHPLASPIGININRAPGTPLEPWLKAYA
                     QFLLSKDVQDIIGSPEMRKVGFRPLTPQEIAAELKKLD"
     gene            complement(25978..>27151)
                     /locus_tag="VFF98_16800"
     CDS             complement(25978..>27151)
                     /locus_tag="VFF98_16800"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZU65350.1"
                     /translation="AALLALPMLPASGWAQAAGPPAAAPVDPALPAYHPRPVPAPHDH
                     AYIMHDGSLRILGFDDMAGMVTRWNALFTRTHPGIRFTPVLRGNGSAIPGITYDTTVF
                     APEGGGATLLELLPYEKIYGSKKDPVAALIIRVGHGSLNPAARMSPLGIIVNKSNPVR
                     YLSQQQVASIFSTGSGTGDFTNWNQIGLGGSLADKPIHPTGLYWDNYMRPEDPNVGEY
                     MMYRKMGAFPGPVFSPNYEQFVHYADVIAKVAADPQAIGIVALNKVTPNVRANVRVVP
                     ILGRDGKTITTGTRAELLAGTYPYARDLYIYIRREPGSAFDPVVKEYMRLILSKEGQQ
                     AIAMDAKGYLPLSAVDVAAELARLDQADSWAPRSKQGPKLNFPFPSPFPSGEAEGQ"
BASE COUNT         4434 a         8895 c         9286 g         4536 t
ORIGIN      
        1 cagcgtgttg ccgggccggg ccgctggcga gcgggggccg gctgccagcc ccagcaccac
       61 catcagcgcc agcccctgcg caagcagggc cgcgccggcc cctgccgccc gctcgtgcca
      121 gcggcgagga caggcggcag aggcgccggc accggcaccg gcaccgggca tcgcccccgc
      181 cccctcagaa ccgccgcgcc aggccgacat tgaccgaacg cccgcgcccg gccaccggcc
      241 gcagcacagc gccgccgctg gcgctgtaat cgcccagcgc caggccgccc agcggcagat
      301 aataggcctt gtcgagtagg ttctcgatgg caaggctgag ccgccagctg ccccgctgat
      361 aggccccgcg caggttgacc agcgcatagg cgggcgtggt cggctcgttg cgggcggtgt
      421 ccacccgggt cttgctgtcc acccagttca gctccagcgt gccggtaaag ccgcccagcg
      481 tatgctccag gctggccatc aggttcagcg gcatctggtg gtagagtggg gtgtggtcgg
      541 tcaggttctg cccgcgcagc cacgacaatg tgccggaaaa ccgcgtttgg ccaaagcgcc
      601 cgccgttcca caccgtggcc gcccccgaga catcgaggcc atagaattgc gcttcctcat
      661 tggcgaattg caactgggcg aaagcgccaa gcgatttgac ataacgcgcg tcgatatagt
      721 cgttcacatg ggtgtagtag ggcgccagtt tcacgaacca gccctgtttg ccccggcccg
      781 aaaaagtgat cgagccgctg atcgtgtcgg cccgttccgg cttcagggca aggttgccga
      841 aatagccgtt gccatcgcca aaccagccga tcatctggct ggacatggcc ccctggcccc
      901 aggtgtagag ctcgtagagg ttgggcgcgc gggccttgtg ggcatagcca agatcgagcg
      961 cgaggccggg cgcgggcgtg taatggccgg tcagcgcgcc gctccagtcg ttgaagtggc
     1021 gctggtggct ggcggcgttg aaggcgctgg cggcggccgc atcggccatg ctcatcatgc
     1081 cggtggaata gggggcgaca tcgccggtgt tcatcgccac cctatcgaac cggccgccgg
     1141 cggtgagggt gaagcggtcg ccccggtgca tctctgcctc gatgtagccg gcgatgcgct
     1201 cgcgctggcc gtgattgacg ttgagatagg tgtcgggccc catcatcatg ctgccggcca
     1261 ctggcggcca ccagtcgtcc agccgctcgt tgcggtattc ctggcccagg cgcaggcggg
     1321 tgccgggcgc caccggcagc tccaccgcca gcgtatagcc accggcgcgc acgcgggtgt
     1381 tcatcggcat gccgccatcg gcgctgccgc ccttgtcggc caggaagttc atctggtgat
     1441 cggtggcgcg gtaatcggcc ttgaggtcca ccgtgcccca gccgaagagg cccagataat
     1501 ggccgttgag gaaccaggaa ttgttcccgg tcaggtccat gaactggttg gggaagccct
     1561 cgtaggcgga atggtgatag ccgccggcca gttccacaag gccgatatcg ccctgatagg
     1621 ccagcgccag ctggtggtct gtcttggcat attggctggc atgcacggtg cccagatcgc
     1681 cgccggcgct gtaattgccg gcccttgtgt agctgccggt ataggtggcc gaaagccggg
     1741 cgccggccac ggtgagggtg aggccggtgc caaagccgcc atcattgctg cggaaataac
     1801 cctgcgcctc gccggtgacg aggaggcggc cggtggtggc aaagcggggc gctgcgcttt
     1861 tcaccgcgat gctcccgccg atggcatcgc ccccggcgct gaccggcggg atgccggtga
     1921 tcaccgcgat cgtgcccacc gtctgcgggt tggtgtagct gagcggcggg ttcatgtcgt
     1981 tggggcaggc ggcctcgatc gggtgcccgt cgacggtgat ggtgaggcgc tgctcgctga
     2041 ggccgcggat caccggcatg gtggaaaagc cgccgccgct gttgccggca acgccgggca
     2101 ggcgggccag cagggcggcg gtgtcgctgg cggcgggggc ggccgcgccg aggtcggccg
     2161 gggtcagggt ggcggcggca tcgctgctgc catcgatatc gaccggcggc agcgccatgg
     2221 cgcccggctg ggccagcgcg gggggggaca cgccggcgat cagcgccagc gcgaggggag
     2281 tggtcttcat gcaacaatcc tgaacacaag gcgcccccgc ccggcaggcc aggggcgcgc
     2341 aacaaaaggc ggggcggctc atggccggcc ccggcgcgca aagggggtgt caggaaagca
     2401 gagggggcgc gcgcagcggt gggcgcaggc gggctggccg ggcgggcaag gccgccgcca
     2461 ccggcgccag cgccgccgcc atggccaaca gcagcgccgc ccacagcagc agcggatctg
     2521 ccccgcccag cagcggcatg gcatggccgg caaaggcgca ggcctgatgg tgatcgccat
     2581 tatgcgcggg cgggcgggcc ggcagggtga tctgccgcag atggggcagg ccgctggcat
     2641 cggcgcagat ctgcacgctc agcacatggg ggccggccgc cggcatataa ccctgcggca
     2701 acagcgcgcg caccgccagc gccagcccga tcagggtgat ggccagccag cgcctggtgc
     2761 gaatcaggat gcgcattgcc tgcatggtgg gcgcggcata cgccctgccg ggccgatcgg
     2821 caaattgacg aatggcctta cttcagcttc catgccaccg ggcaacatat tgggaaacgc
     2881 catgaactcc gatcggctga aagcactgac agatggcgtg gtggccgtca tcatcaccat
     2941 catggtgctg gaaatgaagc cgccgcccag cgctgcgccg gccgccctgc tggcgctggc
     3001 gccggtgttg ctcagctatg tgctcagctt cgtctatgtg gcgatctact ggaataacca
     3061 ccaccatttc ttcgccctca cgccgcgggt gagcgcgggg atcatgtggg ccaatttcaa
     3121 cctgctgttc tggctttcgc tgatcccctt cgccaccgcc tggatgggcg agcacgagct
     3181 gcaggcctgg cccacggcgg tgtatggcgg ggtgctgctg gcctgcgcgc tgtcgtggta
     3241 tctgatgcag agcgtgatca tccgcgcgca ggggccgcaa tcggatctgc gccgggcgat
     3301 cgggcgcgac tggaagggca agttctcgcc gccgctctat gccgccggca tggcgctgtc
     3361 gttctggcgg ccggcgctgg cggcgctgat ctatgccttc gttgccctgc tgtggctggt
     3421 gcccgacagc cgcgtgcatc aggccgccag caggggcgcc taggggatgg gtctgtccac
     3481 taaattatca ttgactaata ttgatgatgc aaaatatatg cggaacacga tttttacaca
     3541 gtgccgcgcc gccggcacag ggaggaacgc catgatcttc ggcctgggaa aaacggcaag
     3601 gcaccaagag atcgaggccc gggcgctggc tgccatggtg gcggccgatg aggtcgtcgt
     3661 ggtcgatgtg cgcgaggcgg acgagtttgc cgccggccat atccccggtg cgctcaacat
     3721 gccgctgtcc gccttccagc cgggcgcgct gccggcccat gccggcaagg cgctggtgct
     3781 taattgccag gggggcaagc gttcgggcat ggcgctggat cgctgcgcgg cggcgcaggc
     3841 ggcggcagat acgcatctgg ccggcggttt tgccgcctgg gtggccgctg gcctgccagt
     3901 cgcgcgctga gccagtcgcg cgctgagcca gcgggccgct ttcagtccca gcgcagcggg
     3961 agggcggtgg ccgcggccac attgccgccc ttcatccgca ccggccgcgc cggatcgagg
     4021 cggaattcgg gcaccgctgc cagccaggcc tggaggaagg cgatcacctc gcgccgggcc
     4081 agcgccgcgc ctacgcagcg gtgcacgccg gcgcccatgg tggtgtggcg cacaggggtc
     4141 aggcggcgtt cgaaatccac ctgttcgggc tgctcgaaac aggccggatc gaggttgtgc
     4201 agcacgctgg gcagatagac gatatcgccc cggcgcacgc gcaccccatc cacctcggca
     4261 tccaccagcg cattgcggct gaccgatacg gtgggatagc ggcggatcag ctcgtcggcg
     4321 gcatcgggga tgagggcagg ctgggcgcgc aggcggcgct ggtcttccgg gtggcgggcc
     4381 aggtgcagcg ccaccatgcc cagcatcgcg gccaccgtgt ccagcccgcc gaacagcagg
     4441 ttgcggcaca ggcgccgtgc ctcgtccagc tgccagggcc gcccgtcgat gggttcggcc
     4501 aggatgcggc tgaacagatc gctgccgggc cgcgccagcc gcgcctcgac atagggggcg
     4561 aggtagtcgt cggcggcctg cttcagctgc tccaccgtga ccgagccatc cggccgggtc
     4621 agctgcaggc ccagttcgcg cagttgcggc cggtcctcga tgggcacgtc gatcatcgag
     4681 aggaagatgt tgatcggcat gatctcggca aaatcgctca cgaattcgca ggcgccgcgc
     4741 ggggccaggg cctctgccag atcgcgcgcg ctggcctcca cataaggctc gagagcggtg
     4801 atatagcgcg aggccatgcc cttcatcacc gccgtgcgat agggcttgtg ctctggcgga
     4861 tcgagctgca gcgggatgaa ggccatcacc tcgcccagcc ccggcgtgac cgccaggcac
     4921 gcgctggaaa agcgggcgtg gtcttcccac agcgcgcgga tcagcgcgcc atcggtggcg
     4981 atccagtggc cgccattggc gctggtccac acaagggcag gcttgccggg gccctgcagg
     5041 ctttgccagg cggcgaaata gtcttcctcc acgccgggcg ggttgaagat gtcgaaatcg
     5101 acgatgcggt tggcggcaag ggcgggcggg ggcagggtgt cggtcatcgg ttcagctccg
     5161 ggccagctcc aggcggtgca gcctctcgcg cagcggccat gcgccccggg ccccggccga
     5221 ctatggcagg cgcggggcgg gagaaaactc gcagtatggc gagggggccg cctaggcggt
     5281 gatcacccct gccgcgccgg cacggaggcg gggaaccagc ggcgccccag ccagaaggcc
     5341 aggttcacca gcaggatcag caccggcacc tcgaccagcg ggccgatcac cgccgcaaag
     5401 gccaccggcg aggccagccc aaaggcggca atggccaccg cgatggccag ttcgaaattg
     5461 ttgccggcgg cggtgaaggc cacggcggtg gtgcgcggat aatcgctgcc gatcagccgg
     5521 cccatggcaa agctgatggt aaactggagg aggaaataga tcgtcagcgg gatagcgatg
     5581 cgcagcgcat cgccgggcag agtgaggatc tcgctgccct tgaggctgaa catggcgacg
     5641 atggtgaaca gcagcgccac cagcgtgaca gggccgatgg ccggcaggaa gcggtgctcg
     5701 taccagtcgg cgccgcgggc cgcgcgcagc catttgcggg tgcagaaacc ggccaggaaa
     5761 ggcacgccca gatagaccag cacagcctgc gcgatcgtct ggaagctgac atgcactacg
     5821 ctgccctcaa ggccgaacaa cggcggcagc accgccagga aaaaccacgc atagaggctg
     5881 aagaacagga tctggaacag cgcgttgaaa gccaccagcg cggcgacata ctggctgtcg
     5941 ccccgggcca gctggttcca cacgatgacc atggcaatgc agcgggccag gccgatcagg
     6001 atcagcccgg tcatgtattc gggcctgtct gccagaaaca gcaccgccag cgcgaacatc
     6061 agcaccggcc cgatgatcca gttctgcacc agcgagagca gcaggatgcg cctgtcgtcg
     6121 aggatatgcg gcagttcctc atagcgcacc cgcgccagtg gcggatacat catcaggatc
     6181 aggccgatgg cgatgggaat gttggtgccg ccccaggaca ggcggcccat ggcggcaggc
     6241 aggccggcaa agctggcccc caggcccacc cccagcgcca tggccaggaa gatccacaag
     6301 gtgaggtagc gatcgaggaa ggagaggcgc tggcgggcgg gggtgtcggg catgatggtt
     6361 ccctggtgtc aggcggcaat gcgcgggtgc atcaagagcc gacgcgccgg ccggcggcat
     6421 cgatcacctg atcgccatct tccttggcga aggcgccgcg ctgggcggcg ggcagcaggt
     6481 cgagcaccgt ttcggagggg cggcacagct tcacgcccag cggcgagacc accagcgggc
     6541 ggttgatgag gatcgggtgg tccatcatcg cgtcgatcag ctggctgtcg gtaagatcgg
     6601 gattgtccag ccccagttcg gcaaagggcg tgcccttttc gcgcagcagc gcgcgcgggc
     6661 tgatctgcgc acgggcgatc aggctttcca gcatcgcgcg tgcgggcggg gtcttgagat
     6721 attccaccac atgcggctcg atgccggcgt tgcggatcat cgccagcgca ttgcgcgatg
     6781 tgccgcaggc ggggttgtga tagatgatga tatcggtgtg catctgcctg ccttcgtttc
     6841 ggtgtcagcc ggcgcagctg agggcggcgc ccagatccgc gcacatttcc ggcgcgccct
     6901 ggcagcaatc ctgcatcagg aaggccagca ggcggcgcat gccggcataa tcggcgcgat
     6961 agcggatcag ccggctttcc cgttccgaat ggacaaggcc ggcccgttcg agcgtggcca
     7021 ggtggtgcga catggtggag ggcggcacgc cggccctttc ggcgatggcg ccggccacat
     7081 gccctgtggg tccggcctgc accagcatgc ggaacaccgc cagccgcgtt tcatgcgcca
     7141 gcgcgcccag cgcatcgacc gcccagaccg ggaggttgat ctcttccata accgctccac
     7201 ctttcgaaca ggatcgaatt atcggccggg ccggatggcg tcaatccatg tttcgagaaa
     7261 tttcgaaatg atgctctgat tccccggcgg cggcgatgcg gggggcgcga cagcgtggcg
     7321 tcagcttggc agggcatagg gaggggcgat caagcctgca ttgattgcct ggagaggaaa
     7381 accccgatga ttcgacacgt gatgaccgcc gccgccttcg tcggcgcgtt ggccgcttcg
     7441 gcggcccacg ccgatgcgcc cgccaagaac tggagcgcgc cggcctacag gatctatgcc
     7501 cagcagcttt ccgacgagac catgcaggcc aaccccgatc tgctgagcgt gactttccac
     7561 ggcatcccgc ccggcgctgt gggcgagacg ttcaccatgt atgccggctc ctatcccgat
     7621 cgcatcggca atccggatga tccggacgat atcgatgtca gcaagaaggg catcaccatc
     7681 gtcgatccgc gctggcaccg caccaaggat ccggccccaa agttcgtggt gctgatgccg
     7741 ctgcgcgatg ccagcatgaa caacatcggc ctgatcgtct atgccttcaa ggtgccggtg
     7801 gaatcgaatg cgggcgagcg cgcattctat gcccgcgcgc ttgatctgcg cgatgcgctg
     7861 gccaggcaga tccccagcta cgaggcgctt tttgcgccgg cgaaataagc cgcgaaaggg
     7921 tgctggcgcc tggcggccat gccggggcgc cggctgcccg cggcaggggc aatggcccgg
     7981 cggatgggcc tgaatatacg cattttcagc tagttgaatg aattctgaaa aaacggcggg
     8041 cgtgtcagca tcgcgccagc aatcggggcc tagacccgga taacgcgctt cgacgcaggt
     8101 ggagatgatg tgatgatcgg cggcgatgtc gagggcggtg cgcaagcgcg ccgtgcccct
     8161 caccactcac tttatatcca gatcctcctg gcgatcctgg cgggcgttgt gctgggcgtg
     8221 ctggcgccgg atgtggcggt gcagtgcaag gtgctgggcg atatgttcgt caggctgatc
     8281 cggatggtga tctcgccggt catcttcctc acggtgtgca caggcattgc cggcatgcgg
     8341 gcgccgggcg cgctggggcg ggtgatggcc aaggcgatgg cctatttcct ggccctgtcg
     8401 acactggcgc tggtgatcgg cctggcggtg gcgctgtttg tgcagccggg cagggggatg
     8461 aatgtcgata tcggcgcgct cgatgggcag tcggtcgccg attatgtcgt caaggctcat
     8521 gcgcaaagcg cgcaaggctt cctcctcgag atcatcccgg attcgctggt gtcgccgctg
     8581 gctggcacga atgttctgca ggcgctggtg gcggccgtgc tgatcggcct gggggtctcg
     8641 ttcatcgggg cgcgggccga gccgctgcgc gccttgctgg atcaggccgc gctggtggtg
     8701 ttccgggtgg ttgaactggt gatgcggctg gcgccggtgg gcactttcgg ggcgatggcc
     8761 ttcaccatcg gcaagttcgg cgcgggcacg attgcgcggc tggcagggct ggttggctgc
     8821 ttctatctca cctcggcgct gttcgtcacg gtggtgctgg gcagcgttgc cctgctcaac
     8881 ggcttctcgc tgtggcggct gctgcgctat ctgcgcgacg agctgctgct ggtgctgggc
     8941 acctcctcct cggaagcggc gctgcccagc ctgatgggca agctggagga ggcggggtgc
     9001 gacaagagcg tggtcgggct ggtggtgccg gcgggctatt cgttcaatct cgatggcacc
     9061 aacatctatc tgacgctggc cgcgctgttt ctggcgcagg cctgcaatgt ccaccttggc
     9121 ctgggccaga tggcgctgct gctgggcgtg gcggtgctca gttccaaggg ggcggcgggc
     9181 gtcacggggg cgggcttcat caccctggcg gccacgctgg ccatcgtgcc cagcgtgccg
     9241 gtggcggcga tggcgctgat cctgggcgtc gaccggttca tgtcgcagtg ccgcagcctc
     9301 accaatttca tcggcaatgc ggtggccacg atcgtcatcg cgcggtggga aggcgggctg
     9361 gagcgcgcgg cgctggaccg ggcgctgggc ggcgcctcca tcctcaccga tgccgggcag
     9421 tttggcatcg cggaatgata aaaaacgcca taacaataac aaggggagag gaaatgatca
     9481 gtcagaacac gcttgccctg gccttgatgc tggctagcac ggcgccggtt gcggcattcg
     9541 cccaggaggc ccccccgcca gagcggcatg ggcaggcttc cgcatcggcc aaggggcacg
     9601 aggcccccac gaatgaggag atcatcgtga ccggccgctt catcgacaca ggcgccaaaa
     9661 gcgcgatgaa gatgccggta tccgtgctcg acaccgcggc aaccgtctcg agctattcca
     9721 gcagctttgt gaaatcgctc gatacgacca ccgtttcgga tctctacaac tacatgaccg
     9781 gcctcaagaa gtcgggcaac acgggctatg acatcaccct gcgcggcttc aagggcagcg
     9841 gcgatgatcg caacacgatc ctggtcgatg gcctgccggg cctcaccgtg cgctatggtt
     9901 cgcccggcac cgtcaacatc gatcatatcg agctggtcca gggcccgacc tcggtgctct
     9961 atggccaggc gcagccgggc ggtttcgtca acctcgtcac caaaagcccg ctcggccatt
    10021 tcgaggccag tgtcgagcag cgcgtcaaca gctatgccgg ctctggcctg ccccttggcc
    10081 ggcgcaatgg catcgaggac gagttcgacg tgaccgggcc gatcacccgg tcgggcaatc
    10141 tgctgttccg cgtgaccggc cagatctcgg atatccagag cttccgcgat ggcgcgctgg
    10201 cttccagttc ggagttcatc gcgccggcgc tgacctggaa gatcaacccg ggcaccagcc
    10261 ttaccgtcaa ggccgaatat ggccatgtca ggcagcatct cgacaatggc gtggtggcac
    10321 cggccaatgg caacatctat gatgtcagcc gcgccgcgcc ccgcaccgct tcctatcagc
    10381 agcccgacga ttttcgcggc gagacgggca agacggtgtc ggccttcctg catcacgatt
    10441 ttggcggcgg ctggcagttc catgcctcct atcgccatgt cagctacaac agcgaccaga
    10501 aatccttcga ccagtatggc acgctgacgg tgcagggcgt tgccgaagtg cagcgccgcg
    10561 cccgcgaact ggtgaccaac cgcagctatg actatggcga tatcaacctc aacggccatt
    10621 tcaagaccgg cttcatcaag cacaagatcc tgatcggcct gaacctgggc agcaacagca
    10681 ccgaaaacaa ccgcatcaag ttcttcaatt ccagcacgcc cgacagcacg accggcaaat
    10741 gccccaccgg cggcacctgc ctcaacattc cggtctataa tccgtccgcc tattacagca
    10801 gcttccccag tttcgacagc ctgccggcga tcaacaccca gctgggcgcg gccgcgcaaa
    10861 aggccctgct caccgatcag ctgtcgaaga ccagaagcta tggcatctat gtctccgatc
    10921 tgatggaact ggcgccctgg ctgaaggtga gcattgccgg gcgcaatttc gccgaaacct
    10981 ccaaggtggc cccggatctg cgcaacctgc ccggcgtctc ctacaagcgc accgaccgca
    11041 ggaacctgct gcccagcgcc ggcgtgctga tcgagccgac ccggcacatg accgtctatg
    11101 cctcctattc ggaatccttc gtgccgtccg atccttcggc gctggatggc aacggcaatg
    11161 tcggcacgct caatccggtc acggccaagc agtatgagct gggcctgaag ggcgagaacc
    11221 tgttccagcg ccggctttcc ttcaccgccg ccctctaccg gatcgatcag gatggccagg
    11281 tgaccaccaa cccctgcatc tatggcactt gctcctatca gacgggcaag ggccgttcgg
    11341 atggctttga agtgcagggc gatgcctcgc ctttcgagaa cctgcagctg ctgttcggct
    11401 acagccacat catcgccaag atccgcacca gcccgaacct gcccttccag gtgggcgatg
    11461 tgctgcccaa tgttgcgcgt gatgccgcca atctgtggag ccgctacaat gtcgggcatg
    11521 ggctggggct gggcctgggc attgtctaca ccggcccccg gcagggggtg ctgcccacca
    11581 gcgccagcga caccaagctg atgccgctgc cgggctatgt cgtggtcgat accgggatct
    11641 actatgatat caaccgctgg tcgatcaacc tcaagatcgg caatctgttc gacaagacct
    11701 atatcgagag cgctggcgcc acgggccgca tccaggccgc ctatggccag ccgcgcaatc
    11761 tcaccctctc ggtcaaagtg aagctgtgag gcagcagggc tcctctggcc gcatcccgca
    11821 gggctggcag gtcgcggcgc tggtcgccgc ggcgctgccg gtcgcggcgc cggcccagga
    11881 tatgccgcaa tatgtgccgc aggcgcaggt cagcggggag atccgcgatt atggctttgg
    11941 gctgggcggt gtgctggcgc agtgggaggc agcgttccgc gcgttccagc cccatgtcac
    12001 cttcaaggac acgctgccca ccagcgatgc ggcctttccg ggcctggtca cctatcaggc
    12061 ggatctgggc atcgatggcg gcgagccggc gatcaccgag gcgctgagct tctgggagac
    12121 gcgcggctat gccgcctcgt ccgtcacggt ggcctcgggc gcctatgacg ttgagggcaa
    12181 gtcgaacggg ccggtggtct ttgtggcgcg cgacaatccg ctgaacagcc tcaccgtcga
    12241 tcagctcgat ggcattttcg gggcggcgcg caatggcacg ctggatggct tcgtgtggag
    12301 cccgcagggc gcgcgcgggg ccgacaggga tatccgcaac tgggggcaac tggggctgcg
    12361 gggcgcctgg gcggcaaagc ccatccagac ctatggccat ggcccctcgg gcacaacgcg
    12421 cttcttccag tggcaggtgc tgggcaatac cgacaagtgg aaccccaatt acaggggcta
    12481 tgtcgagacc ggcagcaagc agatcgccgc gatcgacagg gccggccagc tgctgggcgc
    12541 gcggcacatg ctgggcgagg aactggccca tgaccgctat ggcatcggct ggtccaccat
    12601 gagccaggcc aagggcattg ccggcctcaa gatcctggcc attgccccgc gcggcggcgg
    12661 ggcggcggtg atgcccagcc aggcgacctt cgccgaccgc tcctacccgc tggtgcgcaa
    12721 catctacatc tatttcgacc gccagccggg caccgcgatg gacccgaaac tgcgcgaatt
    12781 cctgcgtttc gtgctcagcg cccagggcca gcggctggtt ggcgcggccg gctacctgcc
    12841 gctgccggcg gccatggtgc tgcgagaacg cacccggctt gatggggggg tggcgccgcc
    12901 cgcccccgcc ccaggaaagg acagaccctg atgtcgcatc atattgcccc gtggcttgcc
    12961 ctggccgcgc tgctggccgc cacgccggcg ctggcagagc cggcccatat cctcatcacc
    13021 gtgccggcgc aggccagggc gccggccgat tttgccacca ccgtggccaa ctggcgccag
    13081 gccggcgaga tcgccgatgc gatctggctg gaggcggacg ggcgcgaggg cggcagcttc
    13141 cagaccttcg tccagctcga ttttcccggc gaggcggagc tgaaggcctg gcaggccagc
    13201 gagggggccc ggctggccgc cccgctggaa gcgcatcgcg tggcgatcct gacccatggc
    13261 gaaacgctgc cgcgcgacag caacctctcc gtcttccagg tggacagttt tcagggcacc
    13321 agcgctgccg gcctggccga ttatgccgcg cattacgtca ggccgctgat ggagggagag
    13381 cgcgccgcca aggtgctgat gcgctacacg gtctataccg agcaccagcc cgatggccac
    13441 gaccgcgcct ggacgctgag cgaatatcac gaccccgccg ctttcgagcg cctgccggcc
    13501 atcgcgcaga atgtctctgc cgcgctcgat cgtgccgatc cggcgtttgc ccggctgggc
    13561 cacagccttg ccgcctatcg cacgcccgag gcgctgacgc gcgcgcgcta tgtggaactg
    13621 ccgccgccgg cgctggctgg cctgcccgat tacaagccgg aatatcacat caccggcacg
    13681 ctgcgcatct atggcagcga gctgaagaat gccgtcgaat atctggcccg cgccttccag
    13741 agctatcagc ccgacatgcg cgtgacctgg agcaacgcca catcgagcga gggcgcgatc
    13801 gccggccttt acaccggcgt ttcggatgtg gcccccatgg gcgatgatgc caagctgacc
    13861 gacgagatgc ccttcttcaa tgtgaacggc tatctgccca cggagatttc ggtggcaaca
    13921 ggcggctatg agcggcgcgg ctcgctctgg gcctgggtca tcgtcgtcaa caaggacaat
    13981 ccgctcaacg agatctcggt tgacgagctg acgcatatct tcggcgccga gcgatcgggc
    14041 ggctgggaac aggcgaacaa caactggctc tacacgtcga aatatgccgt cgacaaagcg
    14101 cagaacctgc gcagctgggg gcagatcggc gtgggcggcg cctatgccgg gcacgagatc
    14161 cagacctatg gcttttccgc cccgggcttt caggccgcct tcgaacggca ctggttccat
    14221 tggtcgcaca agtggaaccc caatctcatg gaatatgtcg agcgcaagga ggcggtcgat
    14281 ggcgcacagg gcgatgctgt ggcgtccgaa cagccgctca aaatcgtctc gaacgacaga
    14341 tacggcatgg ccatcgccgg ccttctccat gtgaaggatt tccccaatct caagatcctg
    14401 aagatctcgg agcacaaggg cgggccctat attcccttca ccccggccac cgttgccaac
    14461 cgcagctatc cgctgcaccg cgacgcctat ttctacgtca acaagcagcc cggccagccg
    14521 ctggatccca aggtgcgcga attcatgcgc ttcgtgctca gccgccaggg ccagcagatc
    14581 atcgccaggg ttggctatta ctatccgatc gatcgcgccg ctctcgagga gcagctgaaa
    14641 aagcttgata attaaacaat aaaagcgggc gagaggagcc ggcaatgaag cgcatgcaag
    14701 caagcggggc gatggccctg atcgcgctgg ccgccaccat gacgagcgcg caggaagaga
    14761 acgcgccaga cagcaagcgg ccgaccggcg agacctatga ccagatcctg catcgcctgg
    14821 gcgatggcat gctggagaac atcgtcatcc gcggcgagca caatgcccgc accgatgccc
    14881 gggcgcacaa gcgcttctac accgacaccg cgccgctggc cgacctgccg ccctatcggc
    14941 ccgggcggca ggtttcgggc acgatccgcg tctcgggcct ctatctgcac gatggcctga
    15001 tcatggatcg ctggatcagg gatttccagc gcttccagcc cggcgcgaag gtgatcatca
    15061 gccagcacgg caccatcgcc tcgggcaagg tggatatcga gacggggccg cgcatcaacg
    15121 accgcctgcg cgcggcatcc gaatatgagc gggcgacagg gctcaggatg ttcgagatcg
    15181 actgggcgac gggctcgtac aatgtgcccg gctggagccc gggcttcgtg atcttcgtcc
    15241 acaaggacaa tcccatcgcg catctctcgc tcgcccagct cgatggcatc ttcgccggcg
    15301 cgcgcacggg cggctggcag ggcacgcaat gggatcccaa ggccgcccgc ggcccggaaa
    15361 agaacatccg cacctggggg cagctgggcc tgacgggcga atgggcgaac aagccgatcc
    15421 acatctatgg ccgcccgctc aaatacaaca tccagctcgg cttcgagcgc aaggtgttcc
    15481 atggcggcga tgtgtggaac gagaacaccg ccgaatacag ccatgagatg aaccccgacc
    15541 acacccgcta cacctcctcg gtggagatgg tgaaggacat ggcggctgat ccctatggca
    15601 tcgtcttttc cgatatgggc tcggcgatgc cggcggtgaa ggcggtgccc atcggcgcca
    15661 ccgacaaggg gcccttcgtg ccgatctcgc tgcagacgct gcgcgaccgc tcctatccgc
    15721 tgttcatcga ggaatgggcc gaggcgcggc tgcagccggg caagccgctc gatccgctgg
    15781 tgaaggaatt cctcaccttc atgcttagcc gcgaggggca ggatgccatc cagcaggatg
    15841 gcaaatggct gccgcttccc gccgcccgcg cccgcgcgat gcgcgcgaag ctggatacga
    15901 ttggcccccg ggcagacccg cgcgcgctgg gcctgcagac cgacatgatc gcgccgccta
    15961 aatgggaggg cgaggcgccc gatgaaaccg gcaggatcga cccgcgccgg gcctattacg
    16021 cgccggattt cgatctgtcg ggcctgccgg cctatgtgcc gtcggcgccg ctttcgggca
    16081 ccatccgcat gccccgcagc ggccagctga tggccagcac catgggccag gcgctgatcg
    16141 ccgccttccg gcgccatcag cccggccttg ccttcgcgct ggccgatggc gacctcatcg
    16201 accatgccgt cgatctgtcc atcggccgca agtggaccag ctatttcgcc ggcgaattct
    16261 atgatttcca gctgaagcat gaccgcagcc cgcgcgaatt cgagatcgcc accggcgctt
    16321 tcgacgtgcc cggctggaac ccggcgatcg ccattctggt caaccgcgcc aaccccatcg
    16381 aggggctgac catccggcag ctggatggca tcttcggcgg cccgcggcgc ggcggctggg
    16441 tgagcaccac ctggcggcgg caggcgggca gggaggcaga gggcaacatc cgcagctggg
    16501 gccagctggg catcaccggc gcctgcgcca gccggccgat cgatgtctat gtgcccccgc
    16561 tcaaatatca ccagatgtcg gtgtttgaac gcaaggtgct ggtgggcggc aacatgtgga
    16621 acgacagcgc gcgcgagatc ccgctggcgc tgcgcaagga catgtcgcgc cgcgtgccca
    16681 gcgccgagcg ggccgctgcc gtggccgccg atccttgcgg catcaccttc gtgtcggccg
    16741 gctttgcgcg ggccggcacc cggctggtgc ccattgccgc cggggccggc cagcgcttcg
    16801 ttgcgcccac gctggcctcg gtgcaggatc gttcctatcc gctgggcctt gaggcctatg
    16861 cctatgccga ccagctgccg ggccagccgc tggatcccgg ggtgagggaa ttcctgcgct
    16921 tcgtgctcag ccgcgagggg caggagatca tcatgcgcga tggcaaatgg ctgccgctga
    16981 ccccggcgct cgccgctgcc cagacagccc gcctcgacga gatcataccg caagtggtcg
    17041 cccccaaggc ggccgcccac aagggggaga caccgcaatg aacaagccgc atgttctggc
    17101 gcagtatgcc gtagcagggg cgctggcctg cgccgtgccg gccgggccgg tttgggccca
    17161 ggaagccgcg ccgcccgcgc ctgcccctgc tgccgccgag gccggcgccg aaaccaatgc
    17221 caaccgcagc atctggaccc tcaacgtcac cagctttcgc aagcagcacg cgctggaggg
    17281 cggctatacc aagcagtggg atctgagcgg cctgccccat tatcacccca cccgccagct
    17341 gaccggcacg ctgcgcatct ggggcagcaa ttatctgaag gatggcccca tcggcgcata
    17401 ttggaggcag gccttcgcca agttccagcc gggcatcacg attgagtaca acctgcccac
    17461 cgccggcatt gccatccccg cgctggcggc caaggtggcg gatctgggcg tggggcgccc
    17521 ggccacgctg atggattatc tcaccttcca gcaggtctat ggcttcaccc agaccgagat
    17581 cgaggtggcg acgggctcct acgatgtcta tggctggtcg ccggcctatg tgatcgtcgt
    17641 caacaaggac aatccgctgg cgcagatcag catgaagcag ctggatggcg ttttcggcac
    17701 cgcgcgcaat ggcggctatg atcacagcgt ctggcgcaca gactatccct tccgccgcgg
    17761 cgcggaagag aacatccgca cctgggggca gctgggcctg acgggcgaat gggcgaacaa
    17821 gccgatccac ccctgcggcc aatcggccca tgccaacatc atgagcgtgt tttccgacaa
    17881 ggtgctgtcg ggcagcgatc agtgggtgga aggctatcgc gcctttgcca attacgcgac
    17941 gcccgaccag aagatcgccg catggaccag ccaggtgcgc aaggcggtcg agggcgatcc
    18001 gctttcgctg tgcgtcatcg cgcccaacgg gctgagcgcg ggcatgcgcg agctggcggt
    18061 gcaggggtgg ggcggcgggc ccttcgttgc gcgctcgctc gaaagcgtgc gcgcgcgctc
    18121 ctatccgctg accaacgaga tctatttcta cgcgatcaac ggcaagggcc acccgatcga
    18181 ccccaaggcc gaggaattcc tgcgcttcgt gctgagccag gaagggcagc aggaaatcca
    18241 gcatgaaggc cgctatctgc cgctgacggc tgcgatggtg caggcccagc tggccaagct
    18301 ggcgccggcc ggccagtgag ccgggcaagc tggcggcgcg ccatcgcgcg ggggctggcc
    18361 ggcgccgcgc tgctggccgg cgtggcgccg gtagcccggg cgcagggcgc cgccgggccg
    18421 ggcctcgatc cggccgccga tcctgccatg agcgccacgc tggccagcct gccgccctat
    18481 caggccgggc cccgggttgc gggcacgatc cgcatctggg gccatggcag ccccacgcat
    18541 gatttcgttg gcgatctggt gcggcgctgg tttgccggct tccaccgcag ccagccggat
    18601 gtggcgctcg actatcgcat gtatggcacg gcctcggcgg tgggcgcgct ggcgacaggg
    18661 gcggccacgc tggcgattct gggcgaggag atcagccccg atgcgcagcg cctgttccgc
    18721 cgcgccaggg gctatgcgcc caccgaagtg gatgttgcca atggcagccg cgatgccaat
    18781 tatttcgatt atgcccatat gatcttcgtc aaccgggcca atccgctcac agggctcacc
    18841 ctgcgccagc tcgaggcggt gttcggcgcc gagcaccgct gcacgcggcg caacctgcgc
    18901 caatggggcg atctggggct gaagggcgat tgggcgcggc gcccgatcac gccctatgcc
    18961 tggaagacgg atgtcgattt tgccctgttc ttccgcgagc gggtgctgtg cggcagccac
    19021 cgctggaacc cggccacgcg tgagttcatg ccggtgacca ggcccgatgg cacggtggtc
    19081 cagcatggcc agctgatcct cgaggcgctg gccaaggacc gctatggcat cgccatttcc
    19141 aatatccgct ttgccaatcc cgccgtgcgc gcgctgccgc tggcgtggcg cgcgaagggc
    19201 ggtttcacca tggccgaccc ggccagcctg atcgcccgca cctatccgct ggggcggatc
    19261 atcccggcct atgtcgatgt gccgcccggc ggccatgtgg gcccggccga gcgggaattc
    19321 ctgcgctata tcctcagccg cgaagggcag caggcgctgg ccgaggccag cggctatctc
    19381 ccgctcgatc gggcaagcca gacccgcgcg ctggcggtgc tgcgatgagg cggccgggca
    19441 ggattggccc gctggtgctg gcatcggcat tgctgctggc accggcgttg ctgccagcgc
    19501 ccgccggcgc ggaactggcg ccgccgcagc ccatgcgccc ggccggcacg ctggtgatcg
    19561 ccaccagcgc cgcgctggcc ccggccttgc gccgcgccgc ccaccgcttc gaggcggcgc
    19621 atgccggcgt gcatgtcctc atcctggggc agggcagcga tgtggcgatg gcccggctct
    19681 acaccggcca ggccgatgcc gcggtgatcg gccgcgatgc cagcgacaat gagatcaagg
    19741 cctatgcctg gatctatggc catgcgccgc aggccgaggc gctgggggcc ggcagccggg
    19801 caacgccggg cctgtcgccc gcgctggcgg tgatcgtggc ggcgcgcagc ccgctgcggg
    19861 cgatgacgct ggaggatctg cgccaggcgc tggccacagc gcccggcccg ttccatatcg
    19921 tcatgcccga tgcgcaaagc ggcaccggcc gtttcctgcg ccaccggctc catcaggatg
    19981 ccgcccagtt ctactggccc cgcgtgcgtg agatcgcgcc gcccgccccc ggccaggccg
    20041 gccgggatgg cgagatcgac cgggcgatcg ccaccgctgt ggccgcagac ccgaaggcgc
    20101 tgggctttgc cgccgcgccg cgccggccgg gcttgcgcgt gctgccgatc cgcgcgggcg
    20161 ggcggctgct gcgcccggca gaccgggatt atccgctgcg ccgcacgatc atggcctatc
    20221 gccctgtggg cgcgcagccg ctgctgatcg cttatctggc ctgcttgcgc ggggtttttc
    20281 cgccggcttc ctgagatttc ccgccgctgc cgcgcggtgt cagcttgccg acagggcggc
    20341 aggctaagcc acagggagac gacacgagga ttcggagagg atatgcgtca gtggcttttt
    20401 gcggcggcgg cctgtgcctg cctgcctttc gcggctttgg ccgataccca tgcggcggcc
    20461 ggcggttcgc ccctgaagat catcggctcg ggcgaattgc ccggctatga gggcgatttc
    20521 gatcatttcg cggtggatac ggccggccag cgcctgttcc tcgccgccga ggaccatggc
    20581 acgctggagg tgttccggct gggctcgctg gctcatctga agacggtgaa gggcccgatc
    20641 gagaccccgc atgccatcct gttcctgccc gatgtgcaca agctgctggt gacagagacc
    20701 gggccaagcc tgccacacta cttcaacgcc gacacctatg ctttcgaaaa gccgctgaag
    20761 ctggtgcagg gcgctgattc gatgggctat gacgcccccc gcaaccgcgc ctatatcgtg
    20821 accggcggca aggaagtgcc gatgccggat tcctggctgg aacaggtcaa tccgcgcacc
    20881 ggggccagga tcaacaaggc gcatttcgct tctgccaacg tgcagggcat ggcggtcgag
    20941 caggccgggc cccatcttta tatcaacgtc agcgatcagc attacatcgc cgtgctcgac
    21001 aaggtgagcc tgaaggagct ggcgcgctgg ccggtcgatc cggcgctggg caccaattgc
    21061 tgctttgcct tcgacgagcc gggccaccgc ctgttcctgg ccacccgcgc gccgggcaac
    21121 ctgctggtgc tggattcgcg cagcggggcg gtgctggccc gcttcaaggc gccggaacgg
    21181 gtggaccaga tcgaatggga tgcccggcat cgccgcgtct atgtgatcgg cggcgagggc
    21241 tggatccaga tcatcgaggc gcgcggcaag gaccagttcg tcgaactgcc ccggcttgac
    21301 accgcccccg gcgccaagac cagcgtgctc gtgccctcgc gcaacgagct ttatgtcgcc
    21361 gtgtcgcccg gtgacagcaa ggcgatggcc agaattctcc gcatttcgac aggacgataa
    21421 gatgcgcaag ctttccatgc ttctggccgc cagccttgcc gttgccggca ccgctgcggc
    21481 cctgcacgcc gaggacaagg cgccggtgcc ggccggcttc ttccgcctgc aatcggtggc
    21541 ccgcctgccg ggcggcgcgc ccgattggga ttacctggcc tatgacagcc agcgccagcg
    21601 cctgttcatc gaccggcgcg acgacgggct gtgggtgttc gacacaaggc tgggcaaggt
    21661 gctgcgccgc atgccgcaca ccgaaggggc aggggcaacg ctgctggtgc ctgccctgcg
    21721 gcgcggcttt gtaaccaatg aggatggctc gactacggtg ttcgacatgg ccagcctggc
    21781 cgtcatcaag cggctgaagt ttgccagcga tgccgatgcg gccagctatg atccggtgag
    21841 cggccgggtg gccttcgtct cggccgacag ccagcaggtc acgctggtcg atccgcgcgg
    21901 cctgaaggtg gtcggcacgc tgaaggtgcc gaccggaaag gcggatggat cggccgccga
    21961 cggggcgggg cacatcctgc tcaacgcgcg cgatcgggca atggtgctgc agatcgatgc
    22021 cgcctcggcc cgggtgctgg cgcaatggcc ggtgaccggc tgcagccagc ccaccggcat
    22081 ggccatcgac actgccagcc accgcgcctt catcggctgc cgcggcgccc agccggtgct
    22141 ggcggtgatg aacacccaga ccggccaggt ggtgcagacg ctcgacatcg ggcgcggcaa
    22201 tgatggcgtc gtctacgatc ccgtccacca ccgcatcctc accaccaacg gcgtggatgc
    22261 gaatatcgtg gcgatccacc aggacgatgc cgaccattac cgcatcgagc aggccgtgac
    22321 gacgcggcct ttcgcgcgca ccatggccta tgatccgcag cgccaggcga tcttcacggt
    22381 caccgccgag ggggtggtcg atccggccca gccggtcaac accgggcccg cccgctttta
    22441 ccccaacgcc tatttccccg gcagcatggt cgtgctgacc tatgcgccga cgcggaaatg
    22501 acagccgctg gcgtttcggg ccccaggccc ggcccgccac caagcgatgc aatggccgcc
    22561 agcgcccgcg aacagggccg cggccgggag aggaccggat catgaatgtg ataactgaaa
    22621 cgcccgccag cgaagggctg cagagcggcg cgatggcgcc tgcccttcct gccgcgcccg
    22681 ccggcgaggg ctgggcgatc ggcgttgtgc cctggccggt ctatctggtg ctggtcgcgg
    22741 tgctggcggc ctttaccgtg gtggggaagg tgcccggcga tatttcggtg atgatcgcgg
    22801 cgctggcggt gggcggcttt gcctgcgccg agctgggcaa gcggctgccg ggcctgtcgc
    22861 aactgggcat ggcggcgatc tttgccactt tcgtgccgtc ctatctttcc tattcgggcc
    22921 tgttgcccaa gctctgggtg aaggcgatca ccaccttcac caaggacagc cagatcctct
    22981 acctctacat cgccgcgatc atcgtcggct ctgttctcag catggaccgc gaggtgctgc
    23041 tgcgcggctt cctgcgcatc ttcgtgccga tggcggcggc cacgctggcg gcggtcgcgg
    23101 tggggctggg ggtgggcatg gcctgcggcc tgtcggcgca tgatgtcttc ttcttcgttc
    23161 tggtgccggc gctgggcggc ggcgtgggcg agggggcgat cccgctctcg atcggctatg
    23221 cgggcctgat gcatcaggaa cagggcctgc tgctggcgcg gattttgccg gcggtgatgt
    23281 tcgccagcct caccgccatc gtcttttccg gcctgatcag cgcctatgcc cgctcgcatc
    23341 cggaactgac cggcaatggc acgctgcagg tggacgagca cgacgagatc ggcctgctca
    23401 acgcccggcc cgaggaagcg gtgacgccgg caggcattgc cggcgccggc gccacggcca
    23461 tcgcccttta tctggtgggc ctgctggggc agagccttct gggctttccc gggccgatca
    23521 ccatgctgat actggcgatc ctgctcaatc tgggccgggt ggtgacagcg cggctgaagg
    23581 cgggttccta tgcctattac cgcttctttt cggttgccgt gacctatccg ctgctctttg
    23641 ccatcggcgt ggcgatgacc ccgtgggaca agctgatgtc ggccttcacc ccggcgatga
    23701 tcctgaccat cgtggccacc gttgccacgc tgatgggcac gggctttgtc gtggggcggc
    23761 tggtcggcat gttcccggtg gaagcggcga tcgtcaccgt gtgccgggcc agccagggcg
    23821 gcaccggcga tgtcgccatc ctcaccgcca gcaaccgcct gcagctgatg ccctttgccc
    23881 agatcgccac ccgcatcggc ggcgccgcga cagtgacgac ggcgctggcg ctgatgaaat
    23941 tgtgggggtg agccatgccg cccccaaacg cttgaccacc aggaggaacg atgaacacgc
    24001 agacactgac aggcgatttg cgccaacccc tgatcggcgc gctggcaaag gtgccgcagg
    24061 tgacgatcgg cttctgggcc atcaagatcc tggcgacaac gctgggcgag accggcgctg
    24121 atctgttcac ccagcaatat gccatgggtt atgccgctgg catcgccttg ttctgcgtgc
    24181 cgctggtggt gctggtggcc gcgcagatca tggccgcatc cttcaacaaa tggctgtatt
    24241 gggcgaccat cctcgcctcc accacggccg gcacggcgct ggccgatttc ttcgatcgct
    24301 cgctgggcat cggttatacg gggggcgcgg ccaccttgct ggcctgtgtg ctgggatcgc
    24361 tgctggcctg gcggctggtg ctgggcacgg tgtctgtggc cagcgttgcc acgcctcggg
    24421 ccgagacctt ctactggatc accatcacct tctcgcagac gctgggcacc gcgctgggcg
    24481 attgggcggc cgatgatggc ggccttggct atgccggcgg cgctgcggtg tttggcggcg
    24541 cgctgctggt gctggcgctg ctctatttcc tgaccggggc cagccgggtc gcgctgttct
    24601 gggccgcatt catcctcacc cggccgctgg gcgccacggt gggcgatttt ctcgacaagc
    24661 cgctggcgca agggggcctc gatctggggc gcgattatgc cacctatgtg ctgatcgggg
    24721 tgatcgcggt ggcgctctgg ttcgtgccgc aaaggcccgg caaggcgcat tgaggaggcc
    24781 tgctgccgaa acacaaaggg ctcaccgccg caccccggcg gtgagccctt tgcttggcct
    24841 ggctggcgct ttggcggtca gtccagcttc ttcagttcgg ctgcgatttc ctgcggggtg
    24901 agggggcgga agcccacctt gcgcatctcg ggcgagccga tgatgtcctg cacgtccttg
    24961 gagagcagga actgggcata ggctttcagc cagggctcca gcggcgtgcc gggcgcgcgg
    25021 ttgatgttga tgccgatcgg cgaggccagc gggtgcagcg gcccttccat cgtgccatcc
    25081 gacagcgtgc ccctgtcgtc gatcaccatc ggcacgaatt tgatccgggg gccaaaatcg
    25141 gccagatgct tccaggtggt caccccgttc atcggcgcgg tgttgttcca atagtccatc
    25201 agcaccatgc cataggcatc ctgcgccacg gcattgatcg cgctttcctc ggtgggcatc
    25261 cattcggtct ggggcgtgaa ggggtggccg ccaaagacct cgcgcatttc atcgatcatc
    25321 gccagaatgg ccgaagtgtc acgcgtgaca tagggatgga tcggcaccgg cccgacatcg
    25381 ccgcccaccc ccagctgcga ccagtgggtg atatcgccct gcttgccgcc cgtggtgaag
    25441 acggcggcgg cctgttccag ggtgatctgt ttgatcggat tgctggcatt gacccacacg
    25501 ccggtcgaca gcttgttgtt cggattgctg tcatagccga tcttgatata ggtcggcgga
    25561 tagccgaagc gctgcgtgaa gccatcaacc tcggcacggc ggttgccgcg gccgatgggc
    25621 ccgaaggccg atttgcccga ggtcagcccc tcgaccgaca gggtggagcc catcatgatc
    25681 ttctggaact tgatgttggg ctccttttgc gcccacagca cgtcgagctt gtcgaacagc
    25741 gggatcagca cgtcattgcc ggccacgaac accgtgtcag gcccgacggt gtaatccttg
    25801 tcggcgggca ccgagagggc tctgggcaca tagggcgcgg gcagcggctc ggcgctgcgg
    25861 gcgatggtgg cagcgccgat caggctgcac agcgcgctgc cggccagggc ggccagcagg
    25921 gcgcgccggg ccggccggcg gcgggcaggg cggaatggag acggaattgt gtgcatctta
    25981 ttggccctcg gcttcgccag acggaaaggg tgacgggaac gggaaattga gctttggccc
    26041 ctgtttggag cgcggcgccc agctatcggc ctgatcgagc ctggccagtt cggccgcgac
    26101 atcgaccgcg ctcagcggca ggtagccctt ggcgtccatg gcgatggcct gctgcccctc
    26161 tttggaaagg atcagccgca tatattcctt gaccacaggg tcgaaggcgc tgcccggttc
    26221 gcggcggatg tagatgtaaa gatcgcgcgc ataggggtag gtgccggcca gcagttcggc
    26281 gcgggtgccg gtggtgatgg tcttgccatc gcgccccagg atcggcacca cccggacatt
    26341 ggccctgaca ttgggcgtga ccttgttgag cgcgacgatg ccgatcgcct ggggatcggc
    26401 ggcgaccttg gcgatcacat cggcataatg cacgaactgc tcgtaattgg gcgagaagac
    26461 cgggcccggg aaggcgccca tcttgcgata catcatgtat tcgccgacat tgggatcctc
    26521 ggggcgcatg taattgtccc agtagaggcc ggtcgggtgg atcggcttgt cggccaggct
    26581 gccgcccagg ccgatctggt tccagttggt gaaatcgccc gtgccgctgc cggtggaaaa
    26641 gatcgaggcg acctgctgct gcgagagata gcggaccgga ttgctcttgt tgacgatgat
    26701 gcccagcggg ctcatccggg cggccgggtt gagcgagcca tggccgacgc gaatgatcag
    26761 cgctgcaacc gggtctttct tcgagccgta gatcttctcg tagggcagca gttccagcaa
    26821 ggtggcgccg ccgccctcgg gggcgaacac ggtggtgtcg taggtgatgc ccggtatcgc
    26881 cgagccattg cccctgagca cgggggtgaa gcggatgccg gggtgggttc gggtgaacag
    26941 cgcgttccag cgcgtcacca tgcccgccat atcgtcgaag cccagaatgc gcagcgatcc
    27001 gtcatgcatg atataggcgt gatcatgcgg cgcgggcacc ggccggggat gataggccgg
    27061 cagcgccgga tcgaccggcg ctgctgctgg cggccctgcg gcttgtgccc atccgctggc
    27121 gggcagcatg ggcagggcaa gcagggcggc c
//
LOCUS       DASRYE010000323        12512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_49153,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000323 DASRYE010000000
VERSION     DASRYE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49153"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFF98_16805"
     CDS             <1..201
                     /locus_tag="VFF98_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HZU65351.1"
                     /translation="KLVYFMSIEEAKFRNPVEPGVLLDLHVSFVQKRSRVCKFRGQAL
                     LEGKVACEVNFTAMIADPPAAA"
     gene            301..534
                     /gene="rpmE"
                     /locus_tag="VFF98_16810"
     CDS             301..534
                     /gene="rpmE"
                     /locus_tag="VFF98_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZU65352.1"
                     /translation="MAKANIHPDYHFITVQMTDGTSFQTRSTWGKEGDTLVLDIDPTS
                     HPAWTGGNQRLLDQGGRVAQFNKRFGGLTLKKG"
     gene            750..826
                     /locus_tag="VFF98_16815"
     tRNA            750..826
                     /locus_tag="VFF98_16815"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:His,seq:gtg)
     gene            1011..1769
                     /gene="ccmC"
                     /locus_tag="VFF98_16820"
     CDS             1011..1769
                     /gene="ccmC"
                     /locus_tag="VFF98_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HZU65353.1"
                     /translation="MHGFANPARFLRMARWLMPLCLLAGLLLALPALAWGVGFGPADR
                     LQGDSVRILYLHVPCAWLGMMGWSGIAVASFTEIVWRHPLAAVAARALALPGAVFTAL
                     CLVTGSLWGRPAWGTWWVWDGRLTSMLVLLFLYFAYMALAHAADDSQEGAPARLPAIF
                     GLVGAVNLPIIHYSVLWWNSLHQPPSITAAVISGKSAMAADFLVPLLVGTLGFSLLFG
                     GVVLARMRMLLANAQAEARLRRKAMAATTSTEAF"
     gene            1771..1929
                     /gene="ccmD"
                     /locus_tag="VFF98_16825"
     CDS             1771..1929
                     /gene="ccmD"
                     /locus_tag="VFF98_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZU65354.1"
                     /translation="MREAFDQWQFVIAAYAIGAAGIAALLGHSLWAMRRAERRRDSII
                     GRSAKRNG"
     gene            1934..2380
                     /gene="ccmE"
                     /locus_tag="VFF98_16830"
     CDS             1934..2380
                     /gene="ccmE"
                     /locus_tag="VFF98_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZU65355.1"
                     /translation="MKAKHQRLVLLSVALVALIGAGLLAIWALRNQASYFYVPADIRA
                     HPPAEGKAVRLGGMVQRGSMHNEADGVTVDFIVGDGKASVPVRFSGIRPALFVEGSGV
                     VAEGHLGADGIFHADNLLAKHDEKYVPREMKNMTADEARKAVAETR"
     gene            2382..4352
                     /locus_tag="VFF98_16835"
     CDS             2382..4352
                     /locus_tag="VFF98_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZU65356.1"
                     /translation="MMAELGLCALWLAAALAGLQLLAGGLSLGAKGSDLAGIIRPVAI
                     VQGLLALAAFGALVLLFVNTDLSVKLVADNSHSAKPLIYKIAGTWGNHEGSMLLWVTV
                     MGLAGACVAVLEHRLPQRTLNATLAAQAFISLGFYAFLLFASNPFARLVPAPEEGNGL
                     NPLLQDPGLAFHPPTLYLGYVGLSVAFSFAVGALVTREVGPAFARAMRPWVLGAWIFL
                     TLGITAGSYWAYYVLGWGGWWFWDPVENASLMPWLAATALLHSASVLAARDALRTWTV
                     MLGVVAFSMSMIGTFLVRSGILTSVHAFAVDPTRGAFILALLAVNIGAALAIFALRAG
                     SVAEGERFAVTSREGALVFNNVMLTSILGIVLLGTLYPLVTEALGEKVSVGPPYFTPT
                     SAIFALPMLLVLVVGPLLRWRRDRLARVRGLLALPALASAAGLVAALVLGVPAGLPLV
                     GLALAPGLAVASLLPLKGRNLAHAPLPLWGMVAAHLGIAISLFGMACDSAFSQETLIA
                     AREGESARVGPWTVRLATVGPIAGPNWTALEARLEAHRADGPVHTLTPQSRSFWAPAQ
                     TTPVSALLTRADGQLYTVLGDEVRDDKGAPTGRWQLRLWWKPFVPAIWLGGLLVALGG
                     VLALLGRVAADIRRRRVEEKIAFRRNHPKGEQ"
     gene            4352..4903
                     /locus_tag="VFF98_16840"
     CDS             4352..4903
                     /locus_tag="VFF98_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZU65357.1"
                     /translation="MAATQGKGVRWALWLPLGLFAAFVALVVVGLLTPKNTDIPSRQI
                     GQALPAFTLPPAADGQPGLAAGDFQGGHPRLLNIFASWCVPCRVEAPVLADLARAGVE
                     VDGIAIRDHKADLAAFLAANGNPYARIGADDISAVQLAIGSSGVPESFVVDAHGVIRY
                     QHIGDIRPEQVPMILQKLKEAGQ"
     gene            4900..5361
                     /locus_tag="VFF98_16845"
     CDS             4900..5361
                     /locus_tag="VFF98_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZU65358.1"
                     /translation="MIRSHLIRSRVIRAAVMPAALLLAATPALADDTMPPAPYAYRQL
                     ADPAQEAKAKALMETLRCLVCQSQSIADSDATIAGDMRNQVRLRIAAGEDPEHIRKWL
                     IARYGDYVSYEPRLSALTWPLFAGPALLLLAGAVMLGRRFGAAQKAEGKGA"
     gene            5358..6002
                     /locus_tag="VFF98_16850"
     CDS             5358..6002
                     /locus_tag="VFF98_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZU65359.1"
                     /translation="MIAWLPIVAVALAAFLALVALRAPRVGWEALAAALLIGLAGYGW
                     QGHPGLAGSPAAGSADAPDMASGMVAERLLLAGDAGQGDKHLIIADALTRHGQYADAI
                     TVLRGATDADPKDSQAWLAMANALVAKAEGNLSPAALLAFRRAAETDPRAPGPPYFLG
                     LALARSGRFEEARGLWLNVVQNAPANAPWRDHIAQRILRLDRLIVLARQMQAQH"
     gene            6135..8072
                     /locus_tag="VFF98_16855"
     CDS             6135..8072
                     /locus_tag="VFF98_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZU65360.1"
                     /translation="MSVAIEGQAAGKQTQVYGPTRPGSEHQHGSLTALAVGAIGVVFG
                     DIGTSPLYAIQAIMTGEHPLPIDILNIYGVISLIFWTMVIVVTLKYVFVVMRCDNNGE
                     GGSLALLALINRKVPGTRGLGLLVVGGLLATALFYADSMLTPAISVISAVEGLDVVST
                     EFHPFIVPIALVILIGLFLIQSRGTEKVGKLFGPVMLVYFAVIAAMGLWNIRLHPQVL
                     WEPLNPLYIWVFFKAHPVISFLSLSAVVYAITGVEALYADMGHFGRQSITLSWLVVVF
                     PALVLNYMGQAALLISTPSASSNPFFNMVPTQLQLPLVVLATLATVIASQAVISGAYS
                     VTQQAIQLGYIPRFRIKHTSHDMAGQIYVPTINWFLACMVAALVISFKSSAAMLPAYG
                     LAVVGTMFITTGMLGVVALRIWKLATWKAGLALAFFLLVDFAYLAAGSAKFFEGAWFP
                     VVVGLLIFSMLTTWAKGRELMRENMAEGSLPLEIFAKSAHSSAVRVPGTAVFLCSSSL
                     VVPSALLHNIKHNKVLHERVVILTVQIEEVPYVPEEKRIAVTEIGQGFYRVVIRFGFL
                     ENTDVPAALKHVTMCGEPFDMMKTSFFLSRQTLISSSKPGMAIWREKLFAWMLRNATS
                     AMEFFHLPINRVVELGSQVEI"
     gene            8195..8815
                     /locus_tag="VFF98_16860"
     CDS             8195..8815
                     /locus_tag="VFF98_16860"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZU65361.1"
                     /translation="MAFVLPELPYAKDAFGSVLSAETFDYHHGKHHNAYVTQANDQVK
                     GTELEGKSLLDVIAAAKAAGNKKLFNASAQIWNHTFYWLGLSPEKQAPSEALAAKIDA
                     DLGGIDALKAALKAESAGHFASGWGWLVLEDGKLKVTSFHDADTPVAYEGVTPLFTLD
                     VWEHAYYVDYRNARPNYLDALLDHVNWAFVEDNLAGTGAAKADQQG"
     gene            complement(8902..9528)
                     /locus_tag="VFF98_16865"
     CDS             complement(8902..9528)
                     /locus_tag="VFF98_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HZU65362.1"
                     /translation="MSEQQAPQAKKSSHWLNLAIDYGPVVAFFIAWRLLKPAGEAAAL
                     PDVVAVTKSTVVFIIATLVALVASKVWRGRVTPMLWLSTVLVVGFGALTIWRQDETWI
                     RHKPTAVYLLFAAMLLAGWWRGRPTLKYLLESAFEGLNELGWMKLSRNWGFFFLFLAA
                     LNEVFAYPGWFTFDQWLRAKLMVFMPASFLFTFAHMPMLLKHGLGEEK"
     gene            complement(9528..10451)
                     /gene="ftsY"
                     /locus_tag="VFF98_16870"
     CDS             complement(9528..10451)
                     /gene="ftsY"
                     /locus_tag="VFF98_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZU65363.1"
                     /translation="MSADSWSSRILGGLAKTSERFTGNLAGIAGGNRLSDDQLDDLED
                     ALILSDVGPRAAARIRGKLAERRFEHGADEAAIRMAVAEEIAEILRPVAKPLDVTAFP
                     RPHVVLVIGVNGSGKTTTIAKLAHVFQEQDYAVMLAAGDTFRAAAIGQLGVWAGRLGV
                     PIVKGPEGGDPASVVFDAVKEATRLGTDALVVDTAGRLQNKRELMDELAKIRRVLGRL
                     NPAAPHDVVLVLDATNGQNALAQIEVFREVAGVTGLVMTKLDGTARGGVLVAAAEQYG
                     LPIHAIGVGETIDDLRPFDPDLVARVIAGVA"
     gene            complement(10448..11617)
                     /locus_tag="VFF98_16875"
     CDS             complement(10448..11617)
                     /locus_tag="VFF98_16875"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HZU65364.1"
                     /translation="MGAEIISLGCRMNLAESERLRALLGAAEDLVVVNSCAVTAEALR
                     QTRQTIRRARRQHPDARLVVTGCAVEVEREAIARLGVDGLVANAAKLDARAWNVPAPA
                     RLLPPPGAHTRAFIPVQNGCDHACTFCIIPAGRGASRSQPVAQVLAEVEAHCAQGARE
                     VVLTGVDLTGWGQDFGQRLGDLVAAILAAFPALPRLRLSSVDGVEIDPLLLELLGSEA
                     RVMPHLHLSLQHGNDLILKRMKRRHSRAQALDLVAGLKARRPEIAVGADIIAGFPTED
                     EAAHADNLSIIAAMGIVHGHIFPYSPRPETPAARMPQLPRPLIAARAAALRAAVAEQR
                     ARWQAGLVGQPAQVLAERDGTGHTPAFARIALPAGTPAGTLLTLTPTHIHEGLLA"
     gene            complement(11622..12446)
                     /gene="dapF"
                     /locus_tag="VFF98_16880"
     CDS             complement(11622..12446)
                     /gene="dapF"
                     /locus_tag="VFF98_16880"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZU65365.1"
                     /translation="MAISFVKMHGLGNDFVVLDGRGQSLPPIDRALASAIANRHTGIG
                     CDQLIVLEPSAGADLRMRIFNPDGGEVEACGNATRAVGLLLGQAASIETLGGRLSVSP
                     AAAGIAAAMGRPRFGWQAIPLAYAMDTLAMPVSWEHEGTTLESPVAVNVGNPHVIFFV
                     ADADAVPLAAMGPVIELDPLFPERVNVNVASVLSRRHIRLRVWERGAGLTRACGTGAC
                     ATAIAAMRRGLVEREVTVSLPGGDLVIGWGADDGITMTGPASEAFRGQFDPAALTA"
BASE COUNT         1899 a         4217 c         4240 g         2156 t
ORIGIN      
        1 aagctggtct atttcatgtc gatcgaggaa gccaagttcc gcaatccggt ggaaccgggc
       61 gtgctgctcg atctgcatgt cagctttgtc cagaagcgtt cgcgggtgtg caagttccgc
      121 gggcaggcgc tgctggaagg caaggtcgcc tgcgaggtga acttcaccgc gatgatcgcc
      181 gatccgccgg ccgcggcctg agaaagcccg ccaaaccgct ggacaaaagc gcggcgcccg
      241 gctaaggcgc cgccttcctt ttcggccggt cggaaccggc aagcgcagaa agagattgtc
      301 atggccaagg ccaatatcca tcccgactat cacttcatca cggtgcagat gaccgacggc
      361 acctccttcc agacccgctc gacctggggc aaggaaggcg acacgctggt gctcgacatc
      421 gaccccacct cgcacccggc gtggaccggc ggcaaccagc gcctgctcga tcagggcggc
      481 cgtgtggccc agttcaacaa gcgcttcggc gggctcaccc tcaagaaggg ctgaggcttt
      541 acgccacggg cgtgccccac aggcacgctt cgggcaacgc gcatgcggcg ggggctttgg
      601 ctcccgccgt tcgcgtttgg ggcgggccgc gccacgggcc cccgcaataa atttcgcgtc
      661 aggcacagat tccctcttcc caagcgcgcc gtctcgtgta aatgcccgcc tcccgacggg
      721 gacgcagatg cactgctcct cctcatgtgg cgattgtagc tcagttggtt agagcgccgg
      781 tttgtggtac cggaggtcga gggttcgaac cccttcaatc gccccatttc ccccttcagc
      841 cttggttccc cttcgcaggc agcatgccgc aggacgcgaa ctggccgaat ttccaatagg
      901 ctggcgcccg cgcccccttg ccgctggccg ccgggcctgc caaagccgct ttctggccac
      961 aagggcctgc gccggcatgg ccggcccccg gtgaggactg ccttttcgcg atgcatggtt
     1021 ttgccaatcc cgcccgtttt ctgcgcatgg cgcgctggct gatgccgctg tgcctgctgg
     1081 ctggcctgtt gctggcgttg ccggcgctgg cctggggcgt gggctttggc ccggcagaca
     1141 ggctgcaggg cgattcggtg cgcatcctct atctgcatgt gccctgcgcc tggctgggga
     1201 tgatgggctg gtcgggcatt gccgtggcca gctttacaga gatcgtctgg cgccatccgc
     1261 tggcggcggt ggccgcgcgc gcgctggcgc tgccgggggc agtgttcacg gcgctgtgcc
     1321 ttgtcaccgg ctcgctgtgg ggccggccgg cctggggcac gtggtgggtg tgggatgggc
     1381 ggctcaccag catgctggtg ctgctgttcc tctatttcgc ctatatggcg ctggcccatg
     1441 cggccgacga cagccaggaa ggcgcgcccg cccgcctgcc ggcgatcttc ggtctggtgg
     1501 gcgcggtgaa cctgccgatc atccattatt cggtgctgtg gtggaacagc ctgcaccagc
     1561 cgcccagcat cacggcggcg gtcatttccg gcaaatcggc catggcggcg gatttcctgg
     1621 tgccgctgct ggtgggcacc ttggggttca gcctgctgtt tggcggcgtg gtgctggcgc
     1681 gcatgcgcat gctgctggcc aacgcccagg cagaggcccg cctgcgccgc aaggccatgg
     1741 ccgccaccac cagcacagag gctttctgac atgcgcgagg catttgatca gtggcaattc
     1801 gtcatcgccg cctatgcgat aggggccgcc ggcatcgccg cgctgctggg tcacagcctg
     1861 tgggccatgc gccgggccga acggcggcgc gacagcatca tcggccgcag cgccaagagg
     1921 aacgggtaag ggcatgaagg ccaagcatca gcggctggtt ttgttgagcg tggcgctggt
     1981 ggcgctgatt ggcgccgggc ttctggcgat ctgggcgctg cgcaatcagg cgagctactt
     2041 ctacgtgccg gccgatatcc gcgcccatcc gccggccgaa ggcaaggcgg tgcggctggg
     2101 cggcatggtg cagcgcggca gcatgcacaa cgaggccgat ggcgtgacgg tggatttcat
     2161 cgtcggcgat ggcaaggcca gcgtgccggt gcgcttctcc ggcatccgcc cggcgctgtt
     2221 cgtggaaggc tctggcgtgg ttgccgaggg ccatctgggc gccgacggca tcttccacgc
     2281 cgacaatctg ctggccaagc atgacgagaa atatgtgccc cgcgaaatga agaacatgac
     2341 agccgacgag gcccgcaagg ccgtggcgga gacgcgctga gatgatggcg gaactgggcc
     2401 tttgcgcttt gtggctggca gcggcgctgg cgggtttgca attgctggca ggcggcctct
     2461 cgctgggggc aaagggcagc gatctggccg gcatcatccg cccggtggcg atcgtgcagg
     2521 ggctgctggc gctggccgcc tttggcgcgc tggtgctgct tttcgtcaac accgatctgt
     2581 cggtgaagct ggtggccgac aacagccatt cggccaaacc gctgatctac aagatcgccg
     2641 gcacatgggg caatcatgaa ggctcgatgc tgctgtgggt gacggtgatg ggccttgccg
     2701 gcgcctgcgt ggcggtgctg gaacatcgcc tgccgcagcg cacgctgaac gcaacgctgg
     2761 ccgcgcaggc cttcatcagc ctgggtttct acgccttcct gctgttcgcc tccaacccct
     2821 tcgcccggct ggtgccggca cctgaagagg gcaatggcct taacccgctg ctgcaggatc
     2881 cgggcctcgc cttccatccg cccacgctgt atctgggcta tgtcggcctg tcggtggcgt
     2941 tcagctttgc cgtgggcgcg ctggtcacgc gggaagtggg gccggccttt gcccgcgcca
     3001 tgcgcccctg ggtgctgggc gcgtggatct tcctcacgct ggggatcacg gcgggcagct
     3061 attgggccta ttacgtgctg ggctggggcg gctggtggtt ctgggatccg gtggagaatg
     3121 cctcgctgat gccctggctg gcggccacgg cgctgctcca ttccgccagc gtgctggccg
     3181 cgcgcgatgc cttgcgcacc tggacggtga tgctgggcgt ggtcgccttt tccatgtcga
     3241 tgatcggcac gttcctcgtg cggtcgggca ttctcaccag cgtccatgcc ttcgcggtgg
     3301 atcccacgcg cggcgccttc atcctggcgc tgctggcggt gaacatcggc gcggcgctgg
     3361 cgatctttgc cctgcgcgcc ggcagcgtgg ccgagggcga gcggtttgcc gtgaccagcc
     3421 gcgagggcgc gctggtgttc aacaatgtca tgctcacctc gatcctcggc attgtcctgc
     3481 tgggcacgct ctatccgctg gtgacagagg cgctgggcga gaaggtttcg gtgggcccgc
     3541 cctatttcac ccccacttcg gcgatcttcg cgctgcccat gctgctggtg ctggttgtcg
     3601 gcccgctgct gcgctggcgg cgcgatcggc tggcgcgggt acgggggctg ctggcgctgc
     3661 cggcgctggc ctcggccgcc gggctggtgg cggcgctggt gctgggcgtg ccggcggggc
     3721 tgccgctggt ggggctggcg ctggcgccgg gcctggccgt ggccagcctg ctgccgctga
     3781 aggggcgcaa tctggcccat gcgccgctgc cgctgtgggg catggtggcc gcgcatctgg
     3841 gtattgccat cagcctgttc ggcatggcct gcgacagcgc cttcagccag gaaacgctga
     3901 tcgccgcgcg cgagggcgag agcgcccgcg tggggccatg gacagtgcgc ctggccaccg
     3961 ttggccccat cgccggcccc aactggaccg cgctggaagc ccgccttgag gcacaccgcg
     4021 ccgatgggcc ggtgcacacg ctcacgccgc aatcgcgcag cttctgggcg ccggcgcaga
     4081 caacccccgt ctcggccctg ctgacccgcg ccgatggcca gctctacacc gtgctgggcg
     4141 atgaagtgcg cgacgacaag ggcgcgccca ccggccgctg gcagctgcgc ctttggtgga
     4201 agcctttcgt gccggccatc tggctgggcg ggctgctggt ggcgctgggc ggcgtgctgg
     4261 cgctgctggg ccgggtggcg gccgatatcc gccgccgccg ggtggaggag aagatcgcct
     4321 tccgccgcaa ccatcccaag ggagagcagt gatggccgcc acacagggca agggtgtgcg
     4381 ctgggcgctg tggctgccgc tgggcctgtt tgccgctttc gtggcgctgg tggtggtggg
     4441 gctgctgacg cccaagaaca ccgacatccc cagccggcag atcggccagg cgctgccggc
     4501 gttcaccctg ccgcccgccg ccgatggcca gccgggcctt gccgcgggcg attttcaggg
     4561 cggccatccg cgcctcctca acatctttgc cagctggtgc gtgccctgcc gggtggaagc
     4621 gccggtgctg gccgatcttg cccgcgcagg cgtggaagtg gacggcatcg ccatccgcga
     4681 tcacaaggcg gatctcgccg ctttcctggc cgccaatggc aacccctatg cccgcatcgg
     4741 cgccgatgac atcagcgcgg tgcagctggc gatcggctcc tcgggggtgc cggaaagctt
     4801 cgtggtcgat gcccatgggg tgatccgcta tcagcatatc ggcgatatcc ggcccgaaca
     4861 ggtgccgatg atcctgcaga agctgaagga ggcgggccaa tgattcgcag ccatctgatt
     4921 cgcagccgcg tgattcgcgc agctgtcatg cccgccgccc tgctgctggc cgccacgccg
     4981 gcgctggccg atgataccat gccgccggcc ccctatgcct atcgccagct ggccgatccg
     5041 gcgcaggagg ccaaggccaa ggcgctgatg gaaacgctgc gctgccttgt ctgccagagc
     5101 cagtcgatcg ccgattcgga tgccaccatc gccggcgaca tgcgcaacca ggtgcgcctg
     5161 cgcatcgccg caggggaaga cccggagcat atccgcaaat ggctgatcgc ccgctatggc
     5221 gattatgtgt cttacgagcc gcggctctcg gcgctcacct ggccgctgtt tgccgggccc
     5281 gcgctgctgc tgctggcagg tgcggtgatg ttggggcgcc gctttggcgc tgcgcaaaag
     5341 gcggaaggga agggcgcatg atcgcctggt tgccgattgt ggccgtggcg ctggcggcgt
     5401 ttctggcgct ggtggcgctg cgcgcgccgc gtgtgggctg ggaggcgctg gccgccgccc
     5461 tgttgatcgg ccttgccggc tatggctggc agggccaccc cggccttgcc ggatcgcccg
     5521 ccgccggcag cgccgatgcg cccgacatgg cctctggcat ggtggccgaa aggctgctgc
     5581 tggccggcga tgcgggccag ggcgacaaac acctcatcat tgccgacgcg ctgacccgcc
     5641 acggccagta tgccgatgcg atcaccgtgc tgcgcggcgc caccgatgcc gatccgaaag
     5701 acagccaggc ctggctggcc atggccaatg cgctggtggc caaggcagag ggcaatcttt
     5761 cgcccgccgc tctgttggcc ttccgtcgcg ccgccgagac agatccgcgc gcgcccggcc
     5821 cgccctattt cctggggctg gcgctggccc gcagcggccg cttcgaggaa gcgcgcggtt
     5881 tgtggctgaa tgtggtgcaa aatgcccccg ccaacgcccc ctggcgcgat cacatcgccc
     5941 agcgcatcct gcgcctcgac cggctgatcg tattggcacg ccagatgcag gcacaacact
     6001 gatcctcgcg cattggccct catgaggcgt gttgcggcgg ctcatgggcc ctgctaaggg
     6061 ccgccggcgt tcatcccgga gcaaggtccg ctgtgcgggc cgccgggatg acaagcgtat
     6121 acaggggcgc tgaaatgagc gttgcgattg aaggtcaggc tgcgggcaaa cagacgcagg
     6181 tatatggtcc gaccaggccc ggttccgaac atcaacatgg cagtctgact gcccttgcgg
     6241 tgggcgcgat cggcgtggtg ttcggcgata tcggcaccag cccgctttat gccatccagg
     6301 cgatcatgac cggcgagcat ccgctgccga tcgatattct caacatctat ggcgttatca
     6361 gcctgatttt ctggacgatg gtgatcgtcg tcacgctgaa atacgtcttc gtcgtcatgc
     6421 gctgcgacaa caacggcgag ggcggcagcc tggcgttgct ggcgctgatc aaccgcaagg
     6481 tgccgggcac gcgcgggctg gggctgctgg tggtgggcgg cctgctggcc accgccctgt
     6541 tctatgccga ctcgatgctc accccggcga tctcggtgat ctcggcggtg gaagggctcg
     6601 acgttgtcag caccgaattc caccccttca tcgtgcccat cgcgctggtc atcctcatcg
     6661 gcctgttcct catccagtca cgcggcaccg agaaggtggg caagctgttc ggcccggtga
     6721 tgctggtcta tttcgcggtg atcgcggcga tgggcctgtg gaacatccgc ctgcacccgc
     6781 aagtgctgtg ggagccgctg aacccgctct acatctgggt gttcttcaag gcccatccgg
     6841 tgatctcgtt cctgtcgctc tcggcggtgg tctatgcgat caccggcgtc gaggcgctct
     6901 atgccgacat gggccatttc ggccgccagt cgatcacgct gtcgtggctg gtggtggtgt
     6961 tcccggcgct ggtgctcaat tatatgggcc aggccgcgct gctcatctcc acgccgtcgg
     7021 cctcgtcgaa ccccttcttc aacatggtgc ccacccagct gcagctgccg ctggtggtgc
     7081 tggccacgct ggccacggtg atcgccagcc aggcggtgat ctcgggcgcc tattcggtca
     7141 cccagcaggc gatccagctg ggctatattc cgcgcttccg catcaagcac accagccacg
     7201 atatggccgg ccagatctat gtgcccacca tcaactggtt ccttgcctgc atggtggccg
     7261 cactggtgat ctcgttcaaa agctcggcgg caatgctgcc ggcctatggc ctggcggtgg
     7321 tgggcacgat gttcatcacc accggcatgc tgggcgtggt ggcgctgcgc atctggaagc
     7381 tggcgacatg gaaggcgggg ctggcactgg ccttcttcct gctggtcgat ttcgcctatc
     7441 tggcggcggg cagcgccaag ttcttcgaag gggcgtggtt cccggtggtg gtgggcctgc
     7501 tcatcttcag catgctgacg acctgggcca agggccgcga gctgatgcgc gagaacatgg
     7561 ccgaaggctc tctgccgctg gaaatcttcg ccaagagcgc ccattccagc gccgtgcgcg
     7621 tgccgggcac ggcggtgttc ctgtgttcct ccagcctcgt cgtgccctcg gcgctgctgc
     7681 acaacatcaa gcacaacaag gtgctgcacg agcgggtggt gatcctcacc gtgcagatcg
     7741 aggaagtgcc ctatgtgccc gaggaaaagc gcatcgccgt caccgagatc ggccagggct
     7801 tctaccgggt ggtgattcgc ttcggcttcc tggaaaacac cgatgtgccc gccgccctca
     7861 agcacgtaac catgtgtggc gagcccttcg acatgatgaa gaccagcttc ttcctctcgc
     7921 gccagacgct gatctcttcc tccaagccgg gcatggccat ctggcgcgaa aagctgttcg
     7981 cctggatgct gcgcaatgcc accagcgcga tggagttctt ccacctgccg atcaaccgcg
     8041 tggtggaact gggcagccag gtggaaatct gagagcggcg ccggctgcat agaaattctt
     8101 tgggggcgcc agcaccgggg gcggttgcgc ccggcgccag cctctctaaa acagcgcctc
     8161 gcggacactt tttccccttg attggagcat agccatggct ttcgtccttc ccgaacttcc
     8221 ctatgccaag gatgctttcg gcagcgtgct gtctgccgag accttcgatt atcaccacgg
     8281 caagcatcac aatgcctatg ttacccaggc gaacgaccag gtgaagggca ccgagctgga
     8341 aggcaagagc ctgctcgacg tgattgccgc cgccaaggcc gccggcaaca agaagctgtt
     8401 caacgcctcg gcgcagatct ggaaccacac cttctactgg ctgggcctca gccccgagaa
     8461 gcaggccccc tcggaagcgc tcgccgccaa gatcgacgct gacctcggcg gcatcgacgc
     8521 gctgaaggcg gcgctcaagg ctgaaagcgc tggccacttt gccagcggct ggggctggct
     8581 ggtgctggaa gacggcaagc tgaaggtgac cagcttccac gacgccgaca cccccgtggc
     8641 ctatgagggc gtgaccccgc tgttcaccct cgatgtctgg gaacacgcct actacgtcga
     8701 ttatcgcaat gcgcgcccga actacctcga cgcgctgctc gaccacgtga actgggcctt
     8761 cgtcgaagac aacctcgccg gcaccggcgc tgccaaggcc gaccagcagg gctgatcgca
     8821 gagcttaagc tacagaataa ccccggtcct tgcgaaaggg ccggggtttt ttctatgcgc
     8881 cagcaaggcg gggaaggggg cctacttctc ctcgcccagc ccgtgcttca gcagcatcgg
     8941 catatgggcg aaggtgaaca ggaaggaggc cggcatgaac accatcagct tggcccgcag
     9001 ccactggtcg aaagtgaacc agccgggata ggcgaacacc tcgttcagcg cggccaggaa
     9061 caggaagaag aagccccaat tgcgcgacag cttcatccag cccagctcgt tcagcccttc
     9121 aaaggcgctt tccagcagat atttcagcgt cggccgcccg cgccaccagc cagccagcag
     9181 catggcggca aacagcaggt aaacggcggt gggcttgtgg cggatccagg tctcgtcctg
     9241 ccgccagatg gtcagcgcgc caaagcccac caccagcacg gtcgacagcc acagcattgg
     9301 cgttacccgc ccgcgccaga ccttcgaggc caccagcgcc accagcgtgg cgataatgaa
     9361 caccaccgtg ctcttggtca cggccaccac atcgggcagc gcggctgcct cgccggccgg
     9421 cttcagcagc cgccaggcga tgaagaaggc caccaccggg ccataatcga tggccaggtt
     9481 cagccagtgc gaggatttct tggcctgcgg ggcctgctgc tcgctcatca cgctaccccc
     9541 gcgatcacgc gcgccaccag atcgggatcg aaggggcgca aatcgtcgat tgtctcgccc
     9601 acgccaatgg catggatggg caggccatat tgctcggccg cggccaccag cacgccgccg
     9661 cgcgcggtgc catccagctt ggtcatgacc aggcccgtca cccccgccac ctcgcggaac
     9721 acctcgatct gggccagcgc gttctggcca ttggtggcat ccagcaccag caccacatca
     9781 tggggcgcgg ccgggttcag ccggcccagc acgcggcgga tcttggccag ctcgtccatt
     9841 agctcgcgct tgttctgcag gcgcccggca gtatccacca ccagcgcatc ggtgcccagc
     9901 cgggtggcct ccttcaccgc gtcgaacacc acgctcgccg ggtctccgcc ttccgggccc
     9961 ttcacgatgg gcacgcccag ccgcccggcc cacacgccca gctggccgat ggccgccgcg
    10021 cggaaggtgt cgccagcggc cagcatcacc gcatagtcct gctcctggaa gacatgggcc
    10081 agcttggcga tggtcgtcgt cttgccgctg ccgttcacgc cgatcaccag caccacatgc
    10141 gggcggggaa aggcggtcac atccaggggc ttggccaccg ggcgcaggat ctcggcgatc
    10201 tcctcggcca cggccatgcg gatggcggcc tcgtcggcac catgttcgaa gcggcgttcg
    10261 gccagcttgc cgcggatgcg ggcggccgcg cgcggcccca catcggagag gatcagcgca
    10321 tcctccagat cgtcgagctg atcgtcggag aggcggttgc cccctgcaat gccggccaga
    10381 ttgccggtga agcgttcgga ggttttcgcc aagccgccga gaatgcggct gctccagctg
    10441 tcggcgctca tgccagaagt ccttcgtgga tatgcgtggg ggtcagggtg agaagcgtgc
    10501 cggcgggcgt gccggcgggc agggcgatgc gggcaaaggc gggcgtgtgg ccggtgccat
    10561 cgcgctcggc cagcacctgc gccggctggc cgacaaggcc cgcctgccag cgcgcgcgct
    10621 gttctgccac ggccgcgcgc aaggcggcgg cgcgcgcggc gatcagcggg cggggcagct
    10681 gcggcatgcg ggccgcaggc gtttccgggc gcggcgaata ggggaagata tggccatgca
    10741 cgatgcccat tgcggcaatg atcgagagat tgtcggcatg ggcggcctcg tcctcggtgg
    10801 ggaagccggc gatgatatcg gcgcccacgg cgatctcggg ccggcgcgcc ttcagccctg
    10861 ccaccagatc cagcgcctgg gcgcggctgt ggcggcgctt catgcgcttg aggatgaggt
    10921 cgttgccatg ctgcagggag aggtgcagat gcggcatcac ccgcgcctcg ctgcccagca
    10981 gctccagcag cagcggatca atctccaccc cgtcgaccga ggacaggcgc aggcgcggca
    11041 gcgccggaaa ggccgccaga atggccgcca ccagatcacc cagccgctgg ccaaaatcct
    11101 gcccccagcc ggtcaggtcc acccccgtca gcaccacttc gcgggcgcct tgggcgcaat
    11161 gggcctccac ctcggccagc acctgcgcca ccggttgcga ccggctggcc ccgcgcccgg
    11221 ccggaatgat gcagaaggtg caggcatggt cgcagccgtt ctgcaccggg atgaaggcgc
    11281 gggtgtgcgc gccgggcggg ggcagcaggc gggccggggc aggcacgttc caggccctgg
    11341 catccagctt ggcggcattg gccaccagcc cgtccacccc cagccgggca atcgcctcgc
    11401 gctccacttc cacagcgcat ccggtcacca ccagccgggc atcgggatgc tggcggcggg
    11461 cgcggcggat ggtctgccgg gtctggcgca gggcctcggc ggtaaccgcg caggagttga
    11521 ccaccaccag atcctccgct gcgcccagca gcgcgcgcag ccgctcgctt tccgccagat
    11581 tcatgcggca gcccagcgag atgatctcgg cccccatcgg ctcaggcggt cagcgctgcg
    11641 gggtcgaact ggccgcggaa agcctcgctg gcagggccgg tcatggtgat gccgtcatcc
    11701 gcgccccagc cgatgacaag atcgccgccg ggcagcgaca ccgtcacttc gcgctccacc
    11761 aggccccggc gcatggcggc aatcgccgtg gcgcaggcgc cggtgccgca ggcgcgggtg
    11821 aggccggcgc cgcgttccca cacccgcagg cggatatgcc tgcgcgagag cacgctggcc
    11881 acattcacgt tcactcgctc gggaaacagc ggatccagct cgatcaccgg gcccatggcc
    11941 gccagcggca cggcatcggc atcggccaca aagaagatca catgcggatt gccgacattc
    12001 accgccaccg ggctttccag cgtggtgccc tcgtgctccc agctcaccgg catggccagc
    12061 gtgtccatcg cataggccag cggaatggcc tgccagccaa agcgcgggcg gcccatggca
    12121 gcggcaatgc ccgccgccgc cggcgagacg gaaagccgcc cgcccagcgt ctcgatgctg
    12181 gcggcctggc ccagcagcag gcccaccgcg cgggtggcat tgccgcaggc ctccacctcg
    12241 ccgccatcgg ggttgaagat gcgcatgcgc agatcggccc cggccgaagg ctccagcacg
    12301 atcagctgat cacagccgat gccggtgtgg cggttggcaa tggcgctggc cagcgcgcga
    12361 tcgatgggcg gcaggctttg cccgcgcccg tcgagcacga cgaaatcatt gcccagccca
    12421 tgcatcttga cgaaggagat agccatatgc ccggccagat aggcgctggg ggctcccgcg
    12481 tccagtggtg cgtccagtgg cgggggcgcc tt
//
LOCUS       DASRYE010000324         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_189114,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000324 DASRYE010000000
VERSION     DASRYE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189114"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFF98_16885"
     CDS             complement(<1..1448)
                     /locus_tag="VFF98_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HZU65366.1"
                     /translation="MGFPQGQGLYDPRNEHDACGVGFVAHIKGEKSHAIITQALEILK
                     NIDHRGAVGADPLLGDGAGILIQLPDALLRDWASGAGVSLPQPGDYGVAMCFLPQDKA
                     SQDFIVETFEKFIAKEGQNLIGWRDVPVSTPGLGKAVLESMPVIRQCIVGRGESCADQ
                     NAFERKLLAIRKQTQNPLEALAAKHGLPGITELYMPSFSTRTIVYKGLLLATQVGSFY
                     DDLTNPLTVSALGLVHQRFSTNTFPSWKLAHPYRFIAHNGEINTVRGNVNWMNARRRT
                     MESELLGPDLDKMWPIIPHGQSDTACLDNALELLVAGGYSLVHAMMMLIPEAWAGNPL
                     MDGKRRAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARFLVTDDDLVVMA
                     SESGVLPIPEEKIVRKWRLQPGRMLLIDFTEGRIIEDEEIKASLAAAEPYEEWLASAQ
                     YNLKALEVVEPELAALPVETTSLLDRQQAFGYT"
     gene            complement(1742..>2633)
                     /locus_tag="VFF98_16890"
     CDS             complement(1742..>2633)
                     /locus_tag="VFF98_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65367.1"
                     /translation="PLGLKARALLALALALCGALAGFGLGRLLGRSHRRAITESEAEA
                     PAMRARAAQPVAQAAAKPEPMRVRRADAHPDAPARRPIRAHEELGEEGLPMPAEAPVI
                     AAAPVIMPEPETAPAVILAPAPATVAEAPALPRMQPLAREAYEMPPAPPASAPEAPEE
                     EAPVGAERLCATPLDHLSQVELVERLAIAMQRRREAAEQAGEGGQQRAPSPATTNIAG
                     ILKAIDAMNGRASANDAGVSGAEEAPAPPAGRWRRSLAVGDSSGWIEEEAEARPAASA
                     PAPDAESTLRSALASLRELR"
BASE COUNT          392 a          886 c          915 g          440 t
ORIGIN      
        1 gtgtagccga aggcctgctg gcgatcgagc aggctggtcg tctccaccgg cagggcagcc
       61 agttccggct ccaccacttc cagcgctttc aggttgtact gcgcgctggc cagccattcc
      121 tcatagggct cggcggcggc caggctcgcc ttgatttcct catcctcgat gatgcggcct
      181 tcggtaaagt cgatcagcag catgcggccg ggctgcagcc gccacttgcg cacgatcttc
      241 tcttccggga tcggcagcac gccgctttcg gaagccatca ccaccagatc atcgtcggtc
      301 acgaggaagc gggcggggcg caggccgttg cggtccagcg tggcgccgat ctggcggcca
      361 tcggtgaagg ccacggcggc cgggccatcc cagggctcca tcagcgcggc gtgatattcg
      421 tagaaggcgc ggcgcttgcc atccatcagc ggattgccgg cccaggcttc cgggatcagc
      481 atcatcatcg cgtggacgag gctgtagcca ccggccacca gcagctccag cgcattgtcg
      541 aggcaggccg tgtccgactg gccatgcggg atgatcggcc acatcttgtc gagatcaggg
      601 cccagcagct cgctttccat cgtgcggcgg cgggcgttca tccagttcac attgccgcgc
      661 acggtgttga tctcgccgtt atgggcgatg aagcgatagg ggtgcgccag cttccagctc
      721 gggaaggtgt tggtcgagaa gcgctggtgc accaggccca gcgccgagac ggtgagcggg
      781 ttggtgagat cgtcgtagaa gctgcccacc tgggttgcca gcagcaggcc cttgtagacg
      841 atggtgcgcg tcgagaagct gggcatgtag agttcggtga tgcccggcag gccgtgcttg
      901 gcggccagcg cctccagcgg gttctgcgtc tgcttgcgga tcgccagcag cttgcgctcg
      961 aaagcgttct ggtcggcgca gctttcgccg cggcccacga tgcactggcg gatcaccggc
     1021 atcgattcga gcacggcctt gcccagcccc ggggtgctga cgggcacatc gcgccagccg
     1081 atcaggttct ggccttcctt ggcgatgaac ttctcgaagg tctcgacgat gaagtcctgg
     1141 ctcgccttgt cctgcggcag gaagcacatt gccacgccat aatcgcccgg ctgcggcagc
     1201 gaaacgcccg cgccgctcgc ccagtcacgc agcagggcat cgggcagctg gatcagaatg
     1261 ccggcgccat cgcccagcag agggtcggcg ccaacagcgc cgcgatggtc gatgtttttc
     1321 agaatttcca gcgcctgggt aatgatggcg tggctttttt cgcctttgat atgggcaaca
     1381 aagcccacac cgcatgcgtc atgctcatta cgcggatcat aaagcccttg gccttgcgga
     1441 aatcccatcg attatctttc ctgcatcatt caggggcgcc accctttgtg tggcgaccac
     1501 gaaaatgaac cctgttctaa aaacgcgaat cgtacgctcg caaagcggca cagatcgcgc
     1561 aggttttccc atgacggccg aacgccgctt ttgcaaccgc gaaagcttgt gccgatgcga
     1621 atcagcgcca gagcgtggca gaagcgccag ccggcccgga gcgccagcct catcatgccg
     1681 gctgggtcat gttgtgcggc cgggcccggg ccagcgcccg cccgcccgcc gccaaacgcg
     1741 cctagcgcag ctcgcgcaag gaggcgagcg ccgagcgcaa ggtgctttcc gcatcggggg
     1801 ccggggcgga ggcggcaggg cgcgcttcgg cctcttcctc gatccagccg ctgctgtcgc
     1861 ccacggccag gctgcggcgc cagcggccgg cgggcggggc gggggcttcc tcggccccgc
     1921 tcacgccggc gtcattggcg ctggcgcggc cgttcatcgc gtcgatcgct ttcagaatgc
     1981 cggcgatatt ggtagtggcg ggcgaaggcg cgcgttgctg gccgccctct ccggcctgct
     2041 cggcagcttc gcggcggcgc tgcatggcaa tcgccaggcg ctccaccagt tccacctgcg
     2101 agagatgatc gagcggggtg gcgcacagcc gttcggcacc caccggcgct tcctcttcag
     2161 gcgcctcggg cgcggaggcg ggcggcgccg gcggcatttc ataggcttcg cgcgccagcg
     2221 gctgcatgcg gggcagggcg ggcgcctcgg ccaccgttgc cggcgcgggc gccaggataa
     2281 cggcaggcgc cgtctcgggc tctggcatga taaccggggc ggcggcgatc accggcgcct
     2341 cggccggcat cggcaggcct tcctcgccca gttcctcatg cgcgcggatc ggccggcggg
     2401 cgggggcatc ggggtgggca tcggcgcggc gcacgcgcat cggctcgggc ttggcggccg
     2461 cctgggcgac aggctgggca gcgcgggcgc gcatggcggg cgcttcggcc tcgctttcgg
     2521 tgatggcgcg gcggtggctg cggcccagca ggcggcccag gccaaaaccg gccagcgcgc
     2581 cgcacagcgc cagcgccagc gccagcagcg cccgcgcctt cagccccagc ggc
//
LOCUS       DASRYE010000325         8774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_172758,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000325 DASRYE010000000
VERSION     DASRYE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172758"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFF98_16895"
     CDS             complement(<1..402)
                     /locus_tag="VFF98_16895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZU65368.1"
                     /translation="MSAAGGLAQRLARAIAAQGPISLVAFMGEANAHYYATRDPLGAA
                     GDFTTAPEIHQMFGEMLGLWLADVWQRAGAPQNAAYVELGPGRGTLAGDALRVMARFG
                     CRPEVHLVETSPALRAAQARVVPVAHWHDDLS"
     gene            complement(399..1265)
                     /gene="lgt"
                     /locus_tag="VFF98_16900"
     CDS             complement(399..1265)
                     /gene="lgt"
                     /locus_tag="VFF98_16900"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZU65369.1"
                     /translation="MAAASSAVRAPIYWEDLGLTRGINLGFFTIRWYSLAYLVGIVLG
                     YLHLSRMIRRPGSPLAQRHVDDLFFWCTLGIIGGGRLGYAIFYGPQLFDHPLDLLKLW
                     QGGMSFHGGFIGVGLAIGYVCWRNGLSFLRVADYIAPCAPFGLFFGRLANFVNGELWG
                     RVAGADVPWAMVFPDRDAGPLPRHPSQLYEAGLEGLALGLIMLVLFWATKARWRPGLM
                     MGTFVLGYGCARFTVEYFREPDVQLAEFAARTGLSMGQWLCVPMILIGLFFAGRALLR
                     PSVQPGRAMPPA"
     gene            1500..4244
                     /locus_tag="VFF98_16905"
     CDS             1500..4244
                     /locus_tag="VFF98_16905"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZU65370.1"
                     /translation="MNAIRISHPRAVIDRRALTVAIGEAVMACGPSARQKVVELLRAA
                     LDAGRTEIARRLAERPSAGHEVAEAQAFLVDQLLRVIYDHVTTTVYPVANRTTGERLT
                     IMAVGGYGRGEMAPHSDVDVAFLMPHKQTSWCEQVIEAMLYFLWDLGLKVGHSSRSLD
                     EMVRMSKSDITICTAMLEGRYVWGDQPLFEEARRRFFTEVVAGTERQFVTEKLAERNE
                     RHKRLGDSRYVVEPNVKEGKGGLRDLHTLYWIGKYIHKVQAPAELVNVGLLSQEEYRA
                     FRRAENFFWAVRCHLHTITRRAEDRLTFDLQRQVAHAMNFSDRPGKSAVERFMQYFFL
                     QAKQVGSLTGVFLAQLDEQFASKQRRGFLARMKDRTRLVKGYKVAGGRIAAPADDWFA
                     RDPIRMLEIFALADENGWEIHPETIRTITRDARLIDADVRREPRANALFMDLLTSRND
                     PETTLRALNEAGVFGRFIPEFGRVNAQMQFDMYHHYTVDEHTIRAIGLLARIEKGELA
                     EDHPLATEIIHKLHSRRTIYVSVLLHDIAKGRGGDHSVLGAEIALRLCPRLGLDDEET
                     LLVSWLVRQHLLMSATAMKRDLADWKTITDFVAVVESLERLRQLVLLTIVDIRAVGPG
                     VWNGWKRQLLQELYRAAEERLRLGHVGYRRPERIAAKQAAVAERLGPERALLDDHGGL
                     FGDAYWIAEPEDVIIMNLRQYDANRKVPLSVTTEYDATRAATLVMVIAADHPGLFYRI
                     AGGIHLAGGNIIDARIHTTRNGIAVDNFLVQDPLGRPFSEKLQLERLKTAIADALANR
                     VKLTPQLAARPLARPRAEAFEVRPRVEFDNDASNRFTVVEVAARDRPALLNRLARALF
                     EARLIVHSAHIATYGERAVDTFYVTDLIGGKVDGASRLRAVEKRLLEAAREESVEEAA
                     "
     gene            complement(4241..5599)
                     /gene="gltX"
                     /locus_tag="VFF98_16910"
     CDS             complement(4241..5599)
                     /gene="gltX"
                     /locus_tag="VFF98_16910"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZU65371.1"
                     /translation="MTTITRFAPSPTGLLHIGNIRTALHNWLLARRAQEAGEGGRFLL
                     RIDDTDQERSREDHVAAIRADLAWLGLDYAGEVRQSARFDVYERAFARLVAEGRVYRC
                     YETAQELDLKRKVLLGRGLPPIYDRAALALTEADHAAQAAAGIAPHWRFLLDHDTPIA
                     WVDGIRGPQHFEARQMSDPVIRRADGSWLYMLPSVIDDIDMGVTQVLRGEDHVTNTAV
                     QVQMFAALGAPAPAFAHEALLTGAEGKLSKRLGATGMAEFRAAGIEPEAIIALLARLG
                     TSDPVDPALGVAELAATFDLSRFGRAPARFDEADLARVNAAILHRLPFAQVAPRLPAG
                     MGAAAWEAIRPNIATLAEAGVWWQRIAEPAPVAFTGEDAAFLATAAEVLAGLAWGEGI
                     WAALTGALKEATGRKGKALFQPLRLALTGEGHGPEMAALLPLIGREAALLRLAAAGAG
                     AA"
     gene            complement(5663..7324)
                     /locus_tag="VFF98_16915"
     CDS             complement(5663..7324)
                     /locus_tag="VFF98_16915"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZU65372.1"
                     /translation="MPDTLKITLAQMNQSVGDLAGNADAILAARARAAEAGADLIAFP
                     ELNLIGYPPEDLVLKPALVERAAAELQRLAQATAGAGPAMLVGSVFVQDGALHNGVAL
                     LDQGRVAATRFKHELPNYGVFDEPRVFQPGPLPEPMIFRGTMIGVPICEDIWHPDVCR
                     HLADFGAEIFLCPNGSPYEIDKDALRIDGVAKRRAVAAKVPLAYLNRVGGQDELVFDG
                     ASFVVNGDGGLAVQMADWEEQEVLTSWTKTATGWRCDRGEIAPQADHAEGIYSAMVLS
                     LRDYVTRNRFPGVVLGLSGGIDSALCAAIAVDALGADKVWCVMLPSRYTSTESLVDAT
                     ECAHMLGLRYDTVPIVPAVKAFDEMLGPVFAGRAPDLAEENLQSRIRGVTLMALSNKF
                     GPMLVTTGNKSEMSVGYATIYGDMNGGYNPLKDAYKTTVFMLAKWRNAHRPRIGLGPN
                     GPVMPDNIISKPPSAELRPDQKDEDSLPPYDLLDAMLMGLVEQEKSVEQLVAEGFARD
                     TLVRIERLLHLAEYKRRQAPPGVKLGSRNFGRDRRYPITQAFRTA"
     gene            complement(7452..>8774)
                     /locus_tag="VFF98_16920"
     CDS             complement(7452..>8774)
                     /locus_tag="VFF98_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZU65373.1"
                     /translation="VHRVPYGTHLLHKTGATVAQGERLAEWDPFTTPIITEKSGVVKY
                     QDLIDGRTLTEQVDEATGMSSRVVTENRASSRGKKEDLRPRLTLLDDASGEAARYMLA
                     PGTTLSVEDGQVVESGDVVARASREAAKTRDITGGLPRVAELFEARIPKDNAVIAKIA
                     GRIEFVRDYKAKRKIAIVPQEGETVEYLIPKSKVIDVQEGDWVKKGDNLISGSPNPHD
                     ILEVLGVEALAEYLCSEIQEVYRLQGVKINDKHIEVIVRQMLQKVEITDGGDTTLLAG
                     EQVDLEEMNETNAKLAEGFRPAEGKPVLLGITKASLQTRSFISAASFQETTRVLTQAA
                     VEGKKDSLIGLKENVIVGRLIPAGTGAGMNRMRVAASSRDAAIRASYRKLQEHLIAPA
                     SAAEEHAAELAQGPEAAGIVDPLAPMEGETHGLDADAGEYLIKGDEAE"
BASE COUNT         1502 a         3064 c         2837 g         1371 t
ORIGIN      
        1 ggaaagatca tcatgccaat gcgcgaccgg caccacacgc gcctgcgccg cgcgcagggc
       61 cggcgaggtt tccaccagat gcacctctgg ccggcagcca aagcgggcca tcacccgcag
      121 cgcatcgccc gccagtgtgc cgcgccccgg ccccagctcc acataggcgg cgttttgcgg
      181 cgcgccggcg cgctgccaca catcggccag ccacaggccc agcatctcgc cgaacatctg
      241 gtggatttcg ggcgcggtgg tgaaatcgcc ggccgccccc agcggatcgc gcgtggcata
      301 gtaatgggcg ttggcctcgc ccatgaaggc caccaggctg atcggcccct gcgccgcgat
      361 ggcccgcgcc agccgctgcg ccagcccgcc tgctgcgctc atgcgggggg catcgcccgg
      421 cccggctgca ccgacggccg cagcagcgcc cggccggcga agaacaggcc gatgaggatc
      481 atcggcacgc acagccactg gcccatcgac aggccggtgc gcgcggcaaa ttcggccagc
      541 tgcacatcgg gctcgcggaa atattccacc gtgaagcgcg cgcagccata gcccagcacg
      601 aaagtgccca tcatcaagcc cggccgccag cgcgccttcg tcgcccagaa cagcaccagc
      661 atgatcagcc ccagcgccag cccttccagc ccggcttcat acagctggct ggggtggcgc
      721 ggcaggggcc cggcatcgcg gtcggggaac accatcgccc agggcacatc ggcgcccgcc
      781 acccggcccc acagctcgcc attgacgaaa ttggccagcc gcccgaagaa caggccaaac
      841 ggcgcacaag gggcgatata atcggccacg cgcaggaagc tgaggccatt gcgccagcac
      901 acatagccga tcgccagccc cacgccgata aagccgccat ggaagctcat gccgccctgc
      961 cacagcttca gcagatcgag cgggtgatcg aacagctgcg ggccatagaa gatcgcatag
     1021 cccagccgcc cgccgccaat gatgcccagc gtgcaccaga agaacagatc atcgacatgg
     1081 cgctgggcca gcgggctgcc cggccggcgg atcatccgcg acaggtgcag atagcccagc
     1141 acaatgccca ccagataagc cagcgaatac cagcggatag tgaaaaagcc gaggttgatg
     1201 ccgcgggtca ggcccagatc ctcccaatag atcggcgccc tcacggcgct ggaagcagcg
     1261 gccatcagca aggcggacaa ggcaggcaag tgaaacgaca tcgcggcaaa cccaagcaag
     1321 ggcccttgcg ggcagacggc ggccttgtgg cacagcacct ttacggatgt aagtgcgttt
     1381 gggatgccag gcgcggccct gcacggcgat gcaccacgcc cctgctaggc gaaagcgcgg
     1441 caaagcggta agaggctgtg ctgccctgca ttcttttccg gtaaaggcgc ccctgacgca
     1501 tgaacgccat tcgcatttcc catccccgcg cggtgatcga ccggcgcgcg cttaccgtgg
     1561 cgatcggcga ggcggtgatg gcctgcgggc cttcggcgcg gcaaaaggtg gtggaactgc
     1621 tgcgcgccgc gctggatgcc ggccgcaccg agatcgcccg gcgcctggcc gagcgcccct
     1681 ctgccggcca tgaagtggcc gaggcgcagg ccttcctcgt cgaccagctg ctgcgggtga
     1741 tctacgacca tgtgacgacg accgtctatc cggtggccaa ccgcaccacc ggcgagcggc
     1801 tgacgatcat ggccgtgggc ggctatggcc ggggcgagat ggcgccccat tccgatgtcg
     1861 atgtcgcctt cctgatgccc cacaagcaaa cctcgtggtg cgagcaggtg atcgaggcga
     1921 tgctctattt cctgtgggat ctgggcctca aggtgggcca ttccagccgc agcctcgatg
     1981 agatggtgcg catgtcgaag agcgacatca ccatctgcac tgccatgctg gaagggcgct
     2041 atgtgtgggg cgaccagcct ttgttcgagg aagcgcgccg ccgcttcttc accgaggtgg
     2101 tggccggcac cgagcgccag ttcgtcaccg aaaagctggc cgagcgcaac gagcgccaca
     2161 agcggctggg cgattcgcgc tatgtggtgg aacccaatgt gaaggagggc aagggcggcc
     2221 tccgggatct ccacacgctc tactggatcg gcaaatatat ccacaaggtg caggcgccgg
     2281 ccgagctggt gaatgtgggc ctgctgagcc aggaggaata tcgcgccttc cgccgggcgg
     2341 aaaacttctt ctgggcggtg cgctgccacc tccacaccat cacccgccgc gccgaagacc
     2401 gcctgacctt cgacctgcag cgccaggtgg cccatgccat gaacttctcc gaccggccgg
     2461 gcaaaagcgc ggtggagcgg ttcatgcagt atttcttcct gcaggccaaa caggtgggca
     2521 gcctcaccgg cgtcttcctc gcccagctcg acgagcagtt tgccagcaaa cagcgccgcg
     2581 gcttcctggc gcggatgaag gaccgcaccc ggctggtgaa gggctacaag gtggccggcg
     2641 gccgcatcgc cgccccggcc gacgactggt tcgcccgcga tcccatccgc atgctggaaa
     2701 tctttgccct ggccgacgag aacggctggg aaatccaccc cgaaaccatc cgcacgatca
     2761 ctcgcgatgc ccgcctgatc gatgccgatg tgcgccgcga gccgcgcgcc aatgccctgt
     2821 tcatggatct gctgaccagc cgcaacgatc ctgaaaccac cctgcgcgcg ctgaacgagg
     2881 cgggcgtgtt cggccgtttc atccccgaat tcggccgggt gaacgcgcag atgcagttcg
     2941 acatgtatca ccactacacg gtggacgaac acaccatccg cgccatcggc ctgctcgccc
     3001 gcatcgagaa gggcgagctg gccgaagacc acccgcttgc caccgagatc atccacaagc
     3061 tgcattcgcg ccgcacgatc tatgtctcgg tgctgctgca cgatatcgcc aaggggcgcg
     3121 ggggcgatca tagcgtgctg ggcgccgaga tcgcgctgcg gctgtgcccg cggctggggc
     3181 tggacgatga ggagacgctg ctcgtcagct ggctggtgcg ccagcacctc ctgatgagcg
     3241 ccaccgccat gaagcgcgac ctggccgact ggaagacgat caccgatttc gtcgccgtgg
     3301 tggaatcgct ggagcggctg cgccagctgg tgctgctcac cattgtcgat atccgcgcgg
     3361 tggggccggg ggtgtggaac ggctggaagc gccagctgct gcaggaactc tatcgcgccg
     3421 ccgaagagcg gctgcgcctg ggccatgtcg gctatcgccg gccagagcgg atcgcggcca
     3481 agcaggccgc cgtggccgag cggctggggc ccgaacgcgc cctgctcgac gatcatggcg
     3541 gcctgttcgg cgatgcctac tggatcgccg agcccgagga tgtcatcatc atgaacctca
     3601 ggcagtatga cgccaaccgc aaggtgccgc taagcgttac caccgaatat gacgccaccc
     3661 gcgccgccac gctggtgatg gtgatcgcgg cagaccatcc gggcctgttc tatcgcatcg
     3721 ccggcggcat tcacctggct ggcggcaaca tcatcgatgc gcgcatccac accacccgca
     3781 acggcatagc ggtggacaat ttcctggtgc aggatccgct gggccgcccc ttctcggaaa
     3841 agctgcagct ggagcggctg aagaccgcga tcgccgatgc gctggccaac cgtgtgaagc
     3901 tgaccccgca gctggccgcc cgcccgctgg cccgcccgcg cgccgaagcc ttcgaggtgc
     3961 gcccgcgcgt ggaattcgac aacgacgcct ccaaccgctt caccgtagtg gaagtggccg
     4021 cgcgcgatcg gcccgcgctg ctcaaccggc tggcccgcgc gctgttcgag gcgcggctga
     4081 tcgtccattc cgcccatatc gccacctatg gcgaacgggc ggtggacacg ttctatgtca
     4141 ccgatctgat cggcggcaag gtggatggcg ccagccgcct gcgcgcggtg gaaaagcgcc
     4201 tgctggaagc ggcccgcgag gaaagcgtgg aggaagccgc ctaggcggcg ccagcgcccg
     4261 ccgccgccag gcgcagcaag gccgcctccc ggccgatcag tggcagcaga gcggccattt
     4321 ccggcccatg cccctcgccc gtcaaggcca ggcgcagggg ctggaacagc gccttgccct
     4381 tgcggccggt ggcttccttc agcgcccctg tcagcgccgc ccagatgccc tcgccccagg
     4441 caaggccagc cagcacttcg gccgctgttg ccaggaatgc cgcgtcctcg cccgtgaaag
     4501 ccaccggcgc cggctctgcg atccgctgcc accacacgcc cgcctcggcc agcgtggcaa
     4561 tgttcgggcg gatcgcctcc cacgccgccg cgcccatgcc ggccggcagg cgcggcgcca
     4621 cctgggcaaa gggcaggcgg tggaggatgg cggcattcac ccgcgccaga tccgcctcgt
     4681 cgaagcgcgc cggcgcccgg ccaaagcgcg acagatcgaa cgtggccgcc agctctgcca
     4741 cccccagcgc cggatcgacc ggatcgctgg tgccaagccg cgccagcagg gcgatgatcg
     4801 cctcgggctc gatgccggcg gcgcgaaatt cagccatgcc ggtggcgccc agccgcttgg
     4861 aaagcttgcc ttccgcgccg gtcagcagcg cctcatgggc aaaggccggg gccggcgcgc
     4921 cgagcgctgc aaacatctgc acctgcacgg cggtgttggt cacatggtcc tcgccgcgca
     4981 gcacctgcgt cacgcccatg tcgatatcgt cgatcaccga gggcagcatg tagagccagg
     5041 agccatcggc gcggcggatc accggatccg acatctgccg cgcctcgaaa tgctggggcc
     5101 cgcgaatgcc atccacccag gcgatcggcg tgtcatgatc gagcaggaag cgccagtggg
     5161 gagcgatgcc ggcggccgcc tgcgcggcat ggtccgcctc ggtcagggcc agcgcggcgc
     5221 ggtcgtaaat cggcggcagg ccgcggccca gcagcacctt gcgcttcagg tccagctcct
     5281 gcgccgtttc atagcagcga tagacgcggc cctcggccac cagccgggca aaggcgcgct
     5341 cgtaaacgtc aaaccgcgcg gactggcgca cttccccggc ataatccagc cccagccagg
     5401 ccagatcggc gcggatcgcc gccacatggt cctcgcggct gcgctcctga tcggtatcgt
     5461 cgatccgcag caggaagcgc ccgccctcgc ccgcctcttg cgcccgccgg gccagcagcc
     5521 agttgtgcag cgccgtgcgg atattgccga tatgaagcag gcccgtgggg ctgggggcaa
     5581 agcgggtgat cgtcgtcatg cgcgggctat aagggggcaa ggggcaaaag gcgagggcca
     5641 acgcccccgc ccgcccctct cctcaggccg tgcggaaggc ctgggtgatc ggatagcgcc
     5701 ggtcgcgccc gaagttgcgg ctgcccagct tcactcccgg cggggcttgg cggcgcttgt
     5761 attcggccag atgcagcagc cgctcgatgc gcaccagcgt gtcgcgcgca aagccctcgg
     5821 ccaccagctg ctccacgctc ttctcctgct ccaccagccc catcagcatg gcatccagca
     5881 ggtcgtaagg cggcaggctg tcctcgtcct tctggtcggg gcgcagctcg gcgctgggcg
     5941 gcttggagat gatattgtcg ggcatcaccg ggccgttggg gccaaggcca atgcgtggcc
     6001 ggtgggcatt gcgccacttc gccagcatga agacggtggt tttataggca tccttcagcg
     6061 ggttatagcc gccgttcatg tcaccataga tggtggcata acccacgctc atctcgctct
     6121 tgttgccggt cgtcaccagc atcgggccga acttgttcga cagcgccatc agcgtcaccc
     6181 cgcggatgcg gctttgcagg ttctcctcgg ccagatcggg cgcgcggccg gcaaacaccg
     6241 ggcccagcat ctcgtcaaac gccttcaccg ccggcacgat cggcaccgtg tcatagcgca
     6301 ggcccagcat atgggcgcat tcggtggcat ccaccaggct ttcggtgctg gtgtagcggc
     6361 tgggcagcat cacgcaccac accttgtcgg cgcccagcgc atccacggcg atcgccgcgc
     6421 acagcgccga atcaatgccg cccgacaggc ccagcaccac gccggggaag cggttgcgcg
     6481 tgacataatc gcgcaaggac agcaccatgg ctgaatagat gccctcggca tggtcggcct
     6541 gcggggcgat ctcgccgcgg tcgcagcgcc agccggtggc ggtcttggtc cagctggtca
     6601 gcacttcctg ctcttcccag tcggccatct gcaccgccag gccgccatcg ccattgacga
     6661 caaagctggc gccatcgaac accagctcat cctgcccgcc cacgcggttg agataggcca
     6721 gcggcacctt ggccgccacc gcccgccgct tggccacgcc atcgatgcgc agcgcatcct
     6781 tgtcgatctc atacgggctg ccattcgggc acaggaagat ttccgcgcca aaatcagcca
     6841 gatggcggca cacatcgggg tgccagatat cctcgcagat gggcacgccg atcatcgtgc
     6901 cgcgaaagat catcggctct ggcagcgggc cgggctggaa cacgcgcggc tcgtcgaaca
     6961 cgccataatt gggcagttcg tgcttgaagc gggtggccgc cacgcgcccc tgatccagca
     7021 gcgccacgcc attgtgcagc gcgccatcct gcacgaacac gctgcccacc agcatggccg
     7081 ggccagcgcc cgccgtggcc tgggccaggc gctgcaattc ggcggccgcc cgctccacca
     7141 gcgccggctt cagcaccaga tcttccggcg gatagccaat gaggttcagc tcgggaaagg
     7201 cgataaggtc ggcgccggcc tccgcggcgc gcgcgcgcgc ggccagaatg gcatcggcat
     7261 tgccggccag atcgccaacg gactggttca tctgggcgag ggtgatcttc agcgtatcgg
     7321 gcatggtggg ggttgtttag ggggccaatc gccggggggc aaggcgatag acaagcccat
     7381 ggcccccaaa gaaaaccccg ccgcagcccg gtggccgcgg cggggtgttt ctggcgcaag
     7441 cgcgaaaagg cttattcggc ctcgtcgccc ttgatcagat attcgccggc atccgcgtcg
     7501 aggccatggg tttccccttc catcggcgcc agcggatcaa cgatccctgc cgcttccggc
     7561 ccctgggcca gctccgccgc atgctcttcg gcagcgctgg caggcgcgat cagatgctcc
     7621 tgcagcttgc gatacgacgc gcgaatggcg gcatcgcggc tggaggcggc cacgcgcatg
     7681 cggttcatgc ccgcgccggt gccggcgggg atgagacggc cgacgatgac gttctccttg
     7741 aggccgatca ggctgtcctt cttgccttcc acggccgcct gcgtgagcac gcgggtggtt
     7801 tcctggaagg acgcggccga gatgaacgaa cgcgtctgca gcgaagcctt ggtgatgccc
     7861 agcagcaccg gcttgccttc ggccgggcgg aagccctcgg ccagcttggc gttggtttcg
     7921 ttcatttctt ccaggtcaac ctgctcacca gccagcaggg tggtgtcgcc gccatcggtg
     7981 atctcaacct tctgcagcat ctggcgaacg atcacctcga tgtgcttgtc gttgatcttc
     8041 acgccctgca agcgatagac ttcctgaatt tccgagcaga ggtattcggc cagcgcctca
     8101 acccccagca cttccagaat gtcatgcggg ttgggcgagc ccgagatcag gttgtcgccc
     8161 ttcttgaccc agtcgccttc ctgaacgtcg atcaccttgc tcttggggat caggtattca
     8221 accgtctcgc cttcctgcgg cacaatggcg atcttgcgct tcgccttgta atcgcggacg
     8281 aattcgatgc gcccggcgat cttggcgatc accgcattat ccttggggat acgcgcttcg
     8341 aacagttcgg ccacacgcgg cagaccgccg gtgatgtcgc gggtcttggc agcttcgcgg
     8401 ctggcacggg ccaccacgtc acccgattcc accacctggc catcttccac cgacaaggtg
     8461 gtgcccggcg ccagcatgta gcgggcagct tcgccgctcg catcatccag cagggtgagg
     8521 cgcggacgca ggtcttcctt cttgccgcgg ctcgaggcgc ggttttcggt caccacgcgg
     8581 ctcgacatgc cggtggcttc gtcgacctgc tcggtcagcg tgcggccgtc gatcaggtcc
     8641 tggtatttca ccacacccga cttttcggtg atgatcggcg tggtgaacgg atcccactcg
     8701 gccagccgct cgccctgcgc cacggtggcg ccggtcttgt gcagcaggtg cgtaccgtaa
     8761 ggcacgcggt gcac
//
LOCUS       DASRYE010000326        17466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_172782,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000326 DASRYE010000000
VERSION     DASRYE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 17466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17466
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172782"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFF98_16925"
     CDS             complement(<1..240)
                     /locus_tag="VFF98_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent oxidoreductase"
                     /protein_id="HZU65374.1"
                     /translation="MTGRSIIVTGGFGVLGQAVSAAFAAAGDRVARVDFAAAPIGGAP
                     AGGLDIGGADLADAATAARVVADVVAAQGGLDVLVN"
     gene            537..1538
                     /locus_tag="VFF98_16930"
     CDS             537..1538
                     /locus_tag="VFF98_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZU65375.1"
                     /translation="MTQTIELAHRLPAGIGLHPLGPGFAAEVTGIDLTGPLSPAQEQA
                     IREMWIAAGVLVFRNQAHDDAAQMRLSRLFGEMEPAATADMNDPDNRFMMTLAYDPED
                     RNPRFQQHYNVGGSDRAGWIGWHWDQSFMPTIVRGAVLRMELPAARMGETGFIDGIGA
                     WERLPGDLKARIEGLEVVYAFNPDFASGQFGFPDDIRPLPRATPGATAGREPQYDFPP
                     VVHPLVITQRETGRKVLKLSPMHARYVLGMARAESDALLAEVARALVDERFAYFHHWQ
                     RNDMVVWDNWRVIHLANGVPLDVPRRARRTTIMGDYKVGRYLDPALDRERQVKRIVD"
     gene            complement(1597..3345)
                     /locus_tag="VFF98_16935"
     CDS             complement(1597..3345)
                     /locus_tag="VFF98_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU65376.1"
                     /translation="MTRQYDMVIRGGTIVDGTGAAPFAGDVAISGGQIAAVGQVDGTG
                     AEEIDASGLIVTPGFVDVHTHYDGQITWENRLAPSSDHGVTTVVMGNCGVGFAPVRPG
                     DHHLMIKLMEGVEDIPEVVMAEGVPFNWSSFPEYLDALDQREADVDFATQIPHSPLRV
                     FVMGQRGADLEPPTLDDLAEMRRCVAEAVNAGALGVASSRNLFHRFRSGALAPSVTTE
                     IDEVVALAQGLRDAGGGIFQCNPNLDNDAETELALFRQITEATGCPVTFSLIHMPTRP
                     DNWSRYIAGIRQARADGLPIRAQFMPRPMGVLYGLDLSFNPFSLNPSFRAIATLPLAE
                     KVAAMRDPALRARLIAEEPEDPNPAFVGLIKAISALYRIQDPADYNFAWEDSLQAEAA
                     RLGKPVREVIYDALLEDEGHAILCAYSSDVPDYLASTADLIGDEGTIVALGDGGAHYG
                     MICDAAYTTYLLTGRMGRDGIDLPRAIRALTSIPAGSVGLNDRGVLAPGYKADVNVID
                     LGRLSLHRPRVKADLPAGGKRLSQKAQGYVATIVSGAVTYREGAATGALPGRLVRGRR
                     EGPATQAADASAKAMA"
     gene            3516..4166
                     /locus_tag="VFF98_16940"
     CDS             3516..4166
                     /locus_tag="VFF98_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU65377.1"
                     /translation="MAQEIAAEAATGENRPPARRPRRTSAEIRARILAAARAEFATRG
                     YAGATTRQIARQADVAEPLVFNNFGSKAALFTEAVIAPFNARFSAFLDASDRMPPDRE
                     LRSAHFVQSLYPFLRENADMLHALVKSAGDRDEPALHGLDDYFTRAVSRMRGQYEAAG
                     LAFDVPPELLVRYAFGMLAGAVLFGDWFFPDAAPDEAAAEAALARMLFKASEPKPG"
     gene            4264..5232
                     /locus_tag="VFF98_16945"
     CDS             4264..5232
                     /locus_tag="VFF98_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65378.1"
                     /translation="MEKIDFSGQVVVVTGAGGALGAAFCRDLARRGAAILANDLGGAP
                     TGAGGTPDYADAIVAEVRAAGGQAVANYDTVATAAGGEAIIAAALSAFGRVDAVISNA
                     GNQRNGAFGALSEADIEAVFAVHAKGAFHVCQPAYRQMVRQGYGRIVLVSSQSGVFGN
                     PFRANYGAAKTALIGLMNVIAQEAPPGIAVNALFPNAQGGRLGGTPLHERPDIAFLEA
                     AGARTRHFAQAMSPAHVAALATYMASRQCDRSQHMYSVLGGKYARIFIGLTQGWYHDG
                     AAPPAAEDVLAQLARIEEAQGYDTPLSGLEEMDTVLAARAALGLGG"
     gene            5229..6116
                     /locus_tag="VFF98_16950"
     CDS             5229..6116
                     /locus_tag="VFF98_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZU65379.1"
                     /translation="MNRADGSPGAAAILDLVATGPGRFTAAGGAANHIGTVFGGRMIG
                     QALCAAVAGVERLPLASLHACFLAPGRLDLPLDYEVLPLRDSRRFANRQVQVRQQGRL
                     IVTLLAAFHAEEDSFAHQAAAMPAVLPPEDVAPLADFIAANAHRLDSAAIRNFGGALP
                     IEMRPIAPEAYFLGRPATPQRAFWFRLKGAAQIADPRLHQCLLAYASDYWLGGAAAIS
                     HALPTNSRVLLISSLDHALWFHRPVRCDEWLLHHTHSPSAGGGQGLVQGQIFTRDGRL
                     VATTTQECLLRRLADAPSG"
     gene            6226..7080
                     /locus_tag="VFF98_16955"
     CDS             6226..7080
                     /locus_tag="VFF98_16955"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZU65380.1"
                     /translation="MVRQRQAEALSPCLGARVTGLDLAEDMRAPTMQALAALLFDRGV
                     VVIPGQRLSPAQYVHFARHFGRPLAFFIPEHRDPDHPEIIHISNDPAIPASQRDGAVH
                     WHSDSSYEAEPGAVTMLLGVEAPDAGGETHFASTSAAYADLPAEMRARLDGLVARHEL
                     GRAPWIEGESQPDPDRPRRATDAPEHPLVMVHPVTGRKGLFTSGTAYAINGMDDAEAT
                     ALIRQLRRHVASPAYRISYKVRPGDLVLWDNFGTVHCASPIAYSNAPGERRLLHRIST
                     KGVPGDVG"
     gene            7070..7891
                     /locus_tag="VFF98_16960"
     CDS             7070..7891
                     /locus_tag="VFF98_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZU65381.1"
                     /translation="MSAEEAAGESILVSRPQADVCLVTLARPARLNALTREMVARLNG
                     ALDAIAADATCRAVILTGAGRGFCSGQDMQAANQRNRTGASGVIEKLHWQEQFAGIGR
                     RLRAMPQLVVAAVNGPAVGAGMALALAADVRLASPAAQFLVAAVRIGLTGGESGLSYL
                     LPRMIGAARAFDILLTGRPLPAAEAERLGLVLALHPPEALVEAALAYARQVLANSPYA
                     VAHTKKLMWENLDAASFEAALAAENRTQILGTFTEDYGEATAAFLEKRPPAFCGR"
     gene            8013..9119
                     /locus_tag="VFF98_16965"
     CDS             8013..9119
                     /locus_tag="VFF98_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU65382.1"
                     /translation="MDGLIETPWPRDRVMPPLSWQRPAGLRVISSDDHLIEPVGLWQD
                     RLKGADRDRAPRYWRDETGFHLEVDGQSYDVPGLPPEFPEGREGFWDVDKRLADMDAE
                     GIDAALIYHGRLNSLIRMPDKAFWLRCVDVCNEWAAEWRAAAPARLFPVALLPTFFTP
                     EATGDYLQKLKALGYKAIDIPVSPKGVRYNSRAMDPMWKAIEDSGLPLSFHIGAYLQY
                     SGRGSLGANLNANLMPFTGLFGQLVFSGVFDRFPALRVVFTEGGASWVAGTLENADKV
                     ARDYASQLRPKLAHKPSWYWFNHCYTTFMEDDVAMEQIDRIGADRVMWSTDYPHPEGV
                     MGHSDAIFRRIHDQLGEAKARRILGANAAQVWGI"
     gene            complement(9187..10761)
                     /locus_tag="VFF98_16970"
     CDS             complement(9187..10761)
                     /locus_tag="VFF98_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU65383.1"
                     /translation="MTDTIPALLRHAARQWGERAALVSQQDGATSYAELDRQADLVAR
                     ALIADGARPGERAAIWAPNMWEWVAVAVGIQRAGGVLVPLNTRLKGGEVADIVRRGGI
                     TRLFAIGTFLGRSYPQMLRGEAMPALRRRIVLRAAPAEMGAGEESLQAFLALAAATSP
                     AALAEREAAVGPDTPSDIMFTSGTTGAPKGALFSHRRSLGGGRAWAGISRQTAEDRYC
                     VFGPFSHNASYKAGWVAGLMTGSTVFWPEAYDAVSILDLIAHNRITVMPAPPTVFQEM
                     LAHPHWRDWDLSSYRFLSTGATVVPIELMKRLQAETAIAEITTGYGMTECCGSATHTR
                     PGDPMERVAYTVGTAIAGTEIRVAGADGRPLPTGQPGEVLIRDDKLLIEYIDNAEASL
                     AAIDAEGWLHSGDVGYLDADGYLTLTDRLKDMYIVGGFNVYPAEIEKQMTGLPGIFQS
                     AIIGVPDQRLGEVGHAFIVRDAGSTITAAEVIAWSKANLANYKVPRGVTFVDALPMNA
                     TGKVTKFALRALLQQG"
     gene            complement(10782..12257)
                     /locus_tag="VFF98_16975"
     CDS             complement(10782..12257)
                     /locus_tag="VFF98_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZU65384.1"
                     /translation="MTDTLLNLASPDKLFIGGDWTAGTGAEQIAIISPASEEVVGRVA
                     KATTADMDRAVAAARHAFDHGPWPRLSGAERAVYLRRIAAEMARRADDFARAWSLQVG
                     MPYAQSAQVAPHMGGYFTYYAGLAEQGFEEVRQPLMGGHAIVVREPVGVVVAVVPWNA
                     PLATLALKVAPALAAGCTVIAKPSPETPLEALILAECVAAAGVPEGVFSVLTADRDVS
                     DHLIRQRAVDKVSFTGSTAAGLHIASVVSGRGGRITTELGGKSAAIVLEDADIATVVA
                     GIMPNLVGLCGQQCAAFSRILVPRARKEDVAAALAAAMAHVAVGDPFAEATQMGPLVT
                     GRQQERVLGYIERGKAEGARVITGGGRPAHLTKGWFVEPTLFAEATNSMTIAREEIFG
                     PVGTIIAYDSEAEAIAIANDSDYGLSGGVFTPDADRAYALARRIRTGNFGHNGRVIDY
                     TIPYGGFKLSGVGREGGVEGLHGFTEVKAIFMAQLPSHLSA"
     gene            complement(12297..13085)
                     /locus_tag="VFF98_16980"
     CDS             complement(12297..13085)
                     /locus_tag="VFF98_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65385.1"
                     /translation="MTALFSLAGRTAMVTGASRGLGKAIARGLAGAGAALVMAARDAG
                     RLEESADELRAAGASVRTIAFDLADLDAVQAATDRLQADGAAVDILVNNGAIGGWGPL
                     HGSTLPHWQTMFDVNVASMYLLCREMSKGMVERRWGRIVNFGSYVSATGRPNLAAYTA
                     SKHAVLGLTRAIAADLAPHGVTCNGIAPGFFATDMAAPTVGHPERARIFRSAIALGDF
                     GDPAQMVGPVLFLASEASAYVTGHMLPVDGGVANILSLPVVVEA"
     gene            13270..14949
                     /locus_tag="VFF98_16985"
     CDS             13270..14949
                     /locus_tag="VFF98_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU65386.1"
                     /translation="MTDARGPAPDAWADYVIVGAGSAGCVLANRLSAGGRHNVAVLEA
                     GGDDRPGRHLLNPAQAASTLNIHLPAGFTRMLKDGRVNWNYLTEADPGTGGRQHVFPR
                     GKVLGGSSSINGMLWVRGLPEDYERWGQMGLAGWNWAEAERYFHRIERQHGAGESAGE
                     GEGLLDIGDVPMQHPMFDAMAQAFAEAGAPVSTDLNGTTREGVARVRVNIRKGRRVSA
                     ATAYLHPAMGRANLRVLTHAHAARVLFEGRRAVGVEYERAGQRQVIRARREVILAGGT
                     INSPQLLELSGIGAVERLAALGIAVVANSPRVGENLQDHFAAMVRARLKPGTPSLNEL
                     SRGGALLGQLLRLAFTGTGLLALGGSNLTAFLKSDGALDLPDLQFFASPATVDFEALA
                     RDGAMTMERQPGMTVGGYVMRPQSVGSIHLRSADFREAPAIRPNFLAHEADCRAQVAC
                     LRIARKVMATPVLAPFFDHELTPGAALESDADLLAFARASGSTGYHQTSTCAMGAGVE
                     AVVSGALKVNGVAGLRVADASVMPFVVSGNTNAATMMIAEKAADLILAEAG"
     gene            14983..16185
                     /locus_tag="VFF98_16990"
     CDS             14983..16185
                     /locus_tag="VFF98_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU65387.1"
                     /translation="MELRPDPALEAFRQEIRAFIRQVLPADMAERQRRKVANNAAVPD
                     LLEWMRILHSHGRSVPHWPVEFGGCGWSPMQLFLFNEELYAADAPEFDWGGTHMLGPV
                     LYTYGSPYLRERFLPMIREGATMWAQGFSEPGSGSDLASLRTSAELVGDTYVVNGQKI
                     WTSGAAHADWGFFLVRTDRSAKPQRGISFLIIRMDTPGITVRQIPQINGEAHLCEVFL
                     DNVQVPADQLIGEPGMGWTYAKFLLEHERTTSSFIYWSKRELARARQIARTEQAGGRR
                     LADDPAIRARFARLEADLIALEWSVLRVLAHEPLAVPEAAAASVLKIRGSALQQEVTM
                     LQADLLGTRALRFFDPAPAPPPAPTPDWPDYVPGRTTVALITRASTIYGGSMQVQKNI
                     LAKLAFGL"
     gene            16203..17321
                     /locus_tag="VFF98_16995"
     CDS             16203..17321
                     /locus_tag="VFF98_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU65388.1"
                     /translation="MDFAQSDIQTMLLDSAQRLLAATAGVEYWRAQRHQPLGFDEARW
                     AQFAELGWLALPVPEAAGGLGGSLEDVALLNVALGEALATEPYVSTAVLADHLLKQAA
                     DRAQGRYLLGEIATGALRVALAHQEVAHQEADGGTLARRDGTGFVLQGAKLLALDAPA
                     AGLLIVSAPIAGEAGLALFLVPADAVGLALEAYPLIDGTRAADLRLEGVALPASALLV
                     GPEHGEAVLAEAIDRASVALMAQAVGAMEAAVQICGRYATERRQFGVAIGSFQAIQHM
                     LADMFVAAHQARSMLYHALAHAEDEAGARGAAIAAARIVVGEAGQLVSRQGIQLHGGY
                     GLTDEYAISHYFRRLMCLEKLYGDLACHVERFGAAVLG"
BASE COUNT         2688 a         5884 c         6143 g         2751 t
ORIGIN      
        1 attcaccagc acatccagcc cgccttgcgc ggccaccaca tcggccacca cgcgggcggc
       61 ggtggcggca tcggcaaggt ctgccccgcc gatatcgagg ccgcccgcgg gtgcgccgcc
      121 gataggagca gcggcaaaat ccacccgcgc cacccggtct ccggcggcgg caaaggcggc
      181 ggagaccgct tggcccagca cgccaaaacc gccggttacg atgatcgaac gtccagtcat
      241 gtcggcaagc tcactccgct ctggccggcc aggccggcga tacatggggg ccggccgggc
      301 cggcgatata tgggggcccg gccgggccgg catccattgg ccgccaaggc tagtacagcg
      361 cggcgggggc ctcaatcggc ggcggcgggg ggcgggcagg gatcagatgg gcgatggccg
      421 ggattattgc cgatcgttga acagtgcttt cagcaatggc cgtattccgc cgttttgccg
      481 ccaccgatag agtgggccgg caagggcgag aacgcccgcc aaggagagga accgacatga
      541 cccagaccat cgagcttgcc caccggctgc ctgcgggcat cggcctgcat ccgctgggcc
      601 cgggctttgc cgccgaagtg accggcatcg acctgaccgg cccgctcagc cccgcccagg
      661 aacaggcgat ccgcgagatg tggatcgctg ccggcgtgct ggtctttcgc aaccaggcgc
      721 atgacgatgc cgcgcaaatg cgcctcagcc gcctgttcgg cgagatggaa ccggccgcca
      781 ccgccgacat gaacgatccc gacaaccgct tcatgatgac gctggcctat gaccccgaag
      841 accgcaaccc gcgcttccag cagcattaca atgtgggcgg cagcgatcgc gccggctgga
      901 tcggctggca ctgggaccag tccttcatgc ccaccatcgt gcggggcgcg gtgctgcgca
      961 tggaactgcc cgccgcgcgc atgggcgaga ccggcttcat cgacgggatc ggcgcctggg
     1021 aacggctgcc gggcgacctc aaggcacgga tcgaggggct ggaagtggtc tatgccttca
     1081 accccgattt cgcctcgggc cagttcggct ttccggacga catccgcccg ctgccccgcg
     1141 ccaccccggg cgctacagcg ggcagagagc cgcaatacga cttcccgccg gtggtgcatc
     1201 cgctggtgat cacccagcgg gaaaccgggc gcaaggtgct caagctgtcg cccatgcatg
     1261 cgcgctatgt gctgggcatg gcgcgggccg aaagcgatgc gctgctggcc gaagtggccc
     1321 gcgcgctggt ggacgagcgc tttgcctatt tccaccactg gcagcgcaac gacatggtgg
     1381 tgtgggacaa ttggcgggtg atccacctgg ccaatggcgt gccgctggat gtgccccgcc
     1441 gcgcccggcg caccaccatc atgggcgatt acaaggtggg ccgctatctc gatccggcgc
     1501 tggaccggga gcggcaggtg aagcgcatcg tcgattgagg cgagaaaggg ggcggcagca
     1561 ctgccgcccc ctttccccgg ggtgctgttg ccgtgttcag gccatggcct tggcgctggc
     1621 atcagcggct tgggtggcag ggccttcacg ccggccacgc accagccggc cgggcagggc
     1681 gccggtggcc gcgccttcgc gataggtgac agcgccgctg acgatagtcg cgacatagcc
     1741 ctgcgccttt tgcgaaagcc gcttgccgcc cgccggcaga tcggccttca cgcgcgggcg
     1801 gtggaggctg agccggccca ggtcgatcac attgacatcg gccttgtagc ccggcgccag
     1861 cacgccgcga tcgttcagcc ccacagagcc ggcgggaatg ctggtgagcg cgcggatcgc
     1921 ccgcggcagg tctatgccat cgcggcccat gcgcccggtg agcagatagg tggtgtaggc
     1981 cgcatcgcag atcatgccgt aatgcgcgcc gccatcgccc agcgcgacaa tggtgccctc
     2041 atcgccgatg agatcggcgg tggaggccag ataatccggc acgtcggaag aataggcgca
     2101 caggatcgca tggccctcgt cttccagcag ggcatcgtag atcacttcgc gcacgggctt
     2161 gcccagccgg gcggcctcgg cctgcaggct gtcttcccag gcgaaattgt agtcggccgg
     2221 atcctggatg cgatagagcg cgctgatcgc cttgatcagg ccgacaaagg cggggttggg
     2281 gtcttccggt tcttcggcga taaggcgcgc gcgcagcgcc ggatcgcgca tcgcggccac
     2341 cttttcggcc agcggcaggg tggcaatggc gcggaagctg gggttcagcg agaaggggtt
     2401 gaagctgaga tcgaggccat agagtacccc catcggccgc ggcatgaact gcgcgcggat
     2461 cggcagccca tcggcccgcg cctggcggat gccggcgata tagcggctcc agttatccgg
     2521 gcgggtgggc atgtggatca ggctgaaggt gaccgggcag ccggtggcct cggtgatctg
     2581 gcggaagagc gccagttcgg tctcggcgtc attgtcgagg ttggggttgc actggaagat
     2641 gccgccgccg gcatcgcgca ggccctgggc cagcgccacc acctcgtcga tctcggtagt
     2701 gacagaaggc gccagcgcgc ccgagcggaa gcggtggaac agattgcgcg aactggccac
     2761 gcccagcgcg ccggcgttca ccgcctcggc cacgcagcgg cgcatttcgg ccaggtcgtc
     2821 cagcgtcggc ggttccagat cggcgccgcg ctggcccatc acgaagacgc gcagcgggct
     2881 gtgcgggatc tgggtggcaa aatcgacatc ggcctcgcgc tggtcgagcg cgtcgagata
     2941 ttcgggaaag ctgctccagt tgaagggcac accttcggcc atcaccactt cggggatgtc
     3001 ttccacgcct tccatcagct tgatcatcag gtgatgatcg cccgggcgca ccggcgcgaa
     3061 gcccacgccg caattgccca tcaccaccgt cgtcacgcca tggtcggagg agggggcaag
     3121 gcggttttcc caggtgatct ggccgtcgta atgggtgtgc acatcgacaa agccgggggt
     3181 cacgatcagc ccgctggcgt cgatctcctc ggcgccggtg ccatccacct ggcccaccgc
     3241 ggcaatctgc ccgcccgaaa tcgccacatc gcctgcaaaa ggcgccgcgc ccgtgccatc
     3301 cacgatcgtg ccgccacgga tcaccatgtc atattgcctg gtcatatcac tccccacatc
     3361 taaacccctg tgccccttga gagcctcttg cacgcatcgg ccggctggcg aatggtgggg
     3421 cgcttgcccg gagcaggggt gcttcttgct ggatttttag atagcaaaca ctatttatca
     3481 tggccggcgg ttttgacaaa ggggaatttg cgcgcatggc acaggagatc gccgcagagg
     3541 ccgcaacggg agaaaaccgg cccccggcgc gccgcccccg gcgcaccagc gccgagatcc
     3601 gcgcgcgcat cctggccgcc gcccgggcgg aatttgcgac tcgcggctat gccggggcca
     3661 ccacccgcca gatcgcccgc caggccgatg tggccgaacc gctcgtcttc aacaatttcg
     3721 gcagcaaggc cgcgctgttc acagaggcgg tgatcgcgcc gttcaatgcc cgcttcagcg
     3781 cctttctcga tgccagcgac cgcatgccgc ccgaccgcga attgcgcagc gcccatttcg
     3841 tgcagtcgct ctatcccttc ctccgcgaga atgccgatat gctgcacgcg ctggtcaaat
     3901 ccgccggcga cagggacgag ccggccctgc acgggctgga cgattacttc acccgcgccg
     3961 tcagccgcat gcgcgggcaa tatgaagcgg cgggcctggc ctttgatgtg ccgcccgaac
     4021 tgctcgtgcg ctatgccttt ggcatgctgg cgggcgcggt gctgtttggc gactggttct
     4081 tccccgatgc cgcgcccgac gaggccgccg ccgaagcggc gctggcccgc atgttgttca
     4141 aggcatcgga acccaagccc ggttgaagcg cccgccatca cattgctgaa atcagccgcc
     4201 cccgcattgg ccccggggcc cccgccagcc atgctgcctg ccgcaacagg caaggcaggc
     4261 agcatggaaa agattgattt cagcgggcag gtggtggtcg tcaccggggc ggggggcgcg
     4321 ctgggggcgg ctttctgccg cgatctggcg cggcgcgggg cggcgatcct ggccaatgat
     4381 ctgggcggcg cgcccacggg cgctggcggc acgcccgatt atgccgatgc catcgtggcc
     4441 gaggtgcgcg cggccggcgg gcaggcggtg gccaattacg acacggtggc aacggccgct
     4501 ggcggcgagg cgatcatcgc ggcggcgctt tcggcgtttg gccgggtgga tgcggtgatc
     4561 tccaatgccg gcaaccagcg caatggtgcc tttggcgcac tgagcgaggc ggatatcgag
     4621 gcggtgtttg ccgttcatgc caagggcgcc ttccatgtgt gccagccggc ctatcggcag
     4681 atggtgcggc agggctatgg gcgcatcgtg ctggtcagtt cgcaatcggg cgttttcggc
     4741 aatccgttcc gcgccaatta cggcgcggcg aaaaccgcgc tgatcgggct gatgaacgtg
     4801 atcgcccagg aagcgccgcc gggcattgcc gtcaacgcgc tgtttcccaa tgcgcagggc
     4861 gggcggctgg gcggcacccc cctgcacgag cggcccgaca tcgcctttct ggaagcggcc
     4921 ggcgcgcgca cccgtcattt cgcccaggcg atgagcccgg cccatgtggc agcgctggcc
     4981 acctatatgg ccagccgcca gtgcgatcgc tcgcagcaca tgtattcggt gttggggggc
     5041 aaatatgcgc ggatcttcat tggcttgacg caaggctggt atcacgatgg cgccgccccg
     5101 ccggcagcgg aggacgtact ggcccagctc gcccggatcg aggaggcgca aggctatgac
     5161 acgccgctga gcgggctgga ggagatggac acggtgctgg ccgcgcgcgc cgcgctgggg
     5221 ctgggcggat gaacagggcc gatggcagcc ccggcgctgc cgccattctc gatctggtgg
     5281 ccacagggcc cggccgcttc acggcggcgg gcggggcggc caaccatatc ggcacggtgt
     5341 ttggcgggcg gatgatcggc caggcgctgt gcgcggcggt ggccggggtg gagcgcctgc
     5401 cgctggcctc gctgcatgcc tgctttctgg cgccgggccg gctggatctg ccgctcgatt
     5461 acgaggtgct gcccctgcgc gacagccgcc gctttgccaa ccggcaggtg caggtgcgcc
     5521 agcaggggcg gttgatcgtc acgctgctgg ccgccttcca tgccgaggag gacagtttcg
     5581 cccatcaggc cgccgccatg cccgccgtgc tcccgcccga ggatgtggcg ccactggccg
     5641 atttcatcgc cgcgaatgct caccggctgg acagcgcggc gatccgcaat ttcggcgggg
     5701 cgctgccgat cgagatgcgc cccatcgcgc ccgaggccta tttccttggc cggccggcca
     5761 cgccgcagcg ggcgttctgg tttcgcctga agggggccgc gcagattgcc gatccacgcc
     5821 tgcaccagtg cctgctggcc tatgccagcg attattggct gggcggcgcg gcggcgatat
     5881 cccacgcgct gcccaccaac agccgggtgc tgctgatctc cagcctcgat catgcgctgt
     5941 ggttccaccg cccggtgcgc tgcgacgaat ggctgctgca ccacacccac agcccttccg
     6001 cgggcggcgg gcaggggctg gtgcaggggc agatcttcac gcgggatggc cggctggtgg
     6061 ccaccaccac gcaggaatgc ctgctgcgcc ggctggccga tgccccctct ggctgagcgg
     6121 ccgcgcatat cacgcatgtg aactgtggcc cgcgccgggg ccatggcgct tgcgcgcggg
     6181 cgcgggcgcg gcactccttg gggcatcaca acacaggagg cgaggatggt gaggcaaagg
     6241 caggcagagg cgctttcgcc ctgccttggc gcgcgggtga cagggctgga tctggccgag
     6301 gacatgcgcg cgccgacgat gcaggcgctg gccgccctgc tgttcgatcg cggcgtggtg
     6361 gtgatccccg gccagcggct gagcccggcg caatatgtgc actttgcccg gcatttcggc
     6421 cgcccgctgg ccttcttcat ccccgagcac cgcgatcccg atcacccgga gatcatccat
     6481 atctccaatg atccggccat tcccgccagc cagcgcgatg gcgccgtgca ctggcattcc
     6541 gacagcagct acgaggccga gcccggcgct gtcaccatgc tgctgggcgt ggaggcgccc
     6601 gatgccggcg gcgagacgca ttttgccagc acatcggccg cctatgccga tctgccggcc
     6661 gagatgcgcg ccaggctgga tgggctggtg gcccgccacg agctgggccg cgcgccctgg
     6721 atcgagggcg agagccagcc cgatcccgat cgcccccgcc gcgccaccga tgcgcccgaa
     6781 catccgctgg tgatggtcca ccccgtcacc ggccgcaagg ggctgttcac ctcgggcacg
     6841 gcctatgcga tcaacggcat ggacgatgcc gaggccaccg cgctgatccg ccagctgcgc
     6901 cgccatgtgg ccagccccgc ctatcgcatc agctacaagg tgcggcccgg cgatctggtg
     6961 ctgtgggaca atttcggcac ggtgcactgc gcctcgccga tcgcgtattc caatgcgccg
     7021 ggcgagcgcc gcctgctcca ccgcatcagc accaaggggg tgccgggcga tgtcggctga
     7081 agaggcggcc ggggagagca tcctcgtcag ccggccgcag gcggatgtct gcctggtcac
     7141 gctggcccgg ccggcgcggc tgaacgcgct gacccgcgaa atggtcgccc ggctgaacgg
     7201 cgcgctcgat gcgattgccg ccgatgccac ctgccgggcg gtgatcctca ccggcgccgg
     7261 gcgaggcttc tgttcgggcc aggacatgca ggccgccaac cagcgcaacc gcaccggcgc
     7321 cagcggcgtg atcgagaagc tgcactggca ggaacagttc gccggcatcg gccgccgcct
     7381 gcgggcgatg ccgcagctgg tggtggcggc ggtgaacggg ccggcggtgg gggcgggcat
     7441 ggcgctggcg ctggcggccg atgtgcgcct ggccagcccg gccgcgcaat tcctggtggc
     7501 ggcggtgcgc attggcctca ccggcgggga aagcggcctc agctatctgc tgccgcggat
     7561 gatcggcgcg gcgcgcgcct tcgacatcct cctcaccggc cgccctttgc cggcagcaga
     7621 ggccgaaagg ctggggctgg tgctggcgct gcacccgccc gaagcgctgg tggaggcggc
     7681 gctggcctat gcccgccagg ttctggccaa cagcccctat gccgtggccc acaccaagaa
     7741 gctgatgtgg gagaacctgg atgcggcctc cttcgaggcg gcgctggcgg ccgagaaccg
     7801 cacgcagatt ctcggcacct tcaccgagga ttacggcgag gccaccgctg ccttcctcga
     7861 aaagcgcccg ccggcctttt gcgggcgctg attcatcgca tgggtgaaca gttcggccgg
     7921 caagcccccg ttcgattgcg cgccgcccgc gcacacataa gcatgatccg cgcatggccc
     7981 tgaggattgg ggcgcatcgg gagagttgag gaatggatgg attgatcgaa accccctggc
     8041 cgcgcgaccg ggtgatgccg ccgctcagct ggcagcggcc cgccggcctg cgggtgatct
     8101 ccagcgacga tcacctgatc gagccggtgg gcctgtggca ggaccggctg aagggcgccg
     8161 accgcgaccg cgcccccaga tactggcgcg acgagaccgg ctttcacctc gaggtggatg
     8221 gccagtccta tgacgtgccg ggcctgccgc cggaatttcc cgaggggcgc gagggcttct
     8281 gggatgtgga caagcgcctg gccgacatgg atgccgaggg gatcgatgcg gcgctcatct
     8341 accacggccg gctgaacagc ctgatccgca tgcccgacaa ggccttctgg ttgcgctgcg
     8401 tggatgtgtg caacgaatgg gcggccgaat ggcgggcggc ggcgcctgcg cgcctgttcc
     8461 cggtggcgct gctgcccacc ttcttcacgc ccgaggccac cggtgattat ctccagaagc
     8521 tgaaggcgct gggctacaag gcgatcgaca tccccgtcag ccccaagggg gtgcgctata
     8581 acagccgggc gatggatccg atgtggaagg cgatcgagga cagcggcctg ccgctttcct
     8641 ttcacatcgg cgcctatctg caatatagcg ggcgcggctc gctgggggcc aatctcaatg
     8701 ccaatctgat gccgttcacc ggcctgttcg gccagctggt gttctccggc gtgttcgacc
     8761 gcttcccggc cttgcgcgtg gtgttcaccg agggcggggc aagctgggtg gccggcacgc
     8821 tggaaaacgc cgacaaggtg gcgcgcgact atgcctcgca attgcgcccg aagctggccc
     8881 acaagcccag ctggtactgg ttcaaccatt gctacaccac cttcatggag gatgatgtgg
     8941 cgatggagca gatcgaccgg atcggcgccg accgggtgat gtggtcgacc gactatcccc
     9001 accccgaagg cgtgatgggc cattccgacg cgatcttccg gcgcatccac gatcagctgg
     9061 gcgaggcgaa ggcgcgccgc atcctgggcg ccaatgccgc ccaggtgtgg ggcatctgat
     9121 ccagggcatg aaaaagggcc ggcggatgtg ccgccggccc tttcccgttc gccccggccc
     9181 cccgtttcag ccctgttgca acagcgcgcg cagggcgaat ttggtgacct tgccggtggc
     9241 attcatcggc agcgcatcca caaaggtaac cccgcgcggc accttgtaat tggccaggtt
     9301 cgccttgctc caggcgatca cttcggcagc ggtgatggta gagccggcat cgcgcacgat
     9361 aaaggcatgg cccacctcgc ccagccgctg atcgggcacg ccgatgatgg ccgactggaa
     9421 gatgccgggc agccccgtca tctgcttctc gatctcggcc ggatagacgt tgaacccgcc
     9481 gacgatatac atgtccttca gccggtcggt cagcgtgaga tagccatcgg catccagata
     9541 gccgacatcg ccggaatgca gccagccctc ggcatcgatg gcggccaggc tggcttcggc
     9601 attgtcgata tattcgatca gcagcttgtc gtcgcggatc agcacttcgc cgggctggcc
     9661 ggtgggcagc gggcggccat cggcgccggc cacgcggatt tcggtgccgg ctatggcggt
     9721 gcccaccgtg taggccacgc gttccatcgg atcgcccggg cgggtgtggg tggccgagcc
     9781 gcagcattcg gtcatgccat agccggtagt gatctcggcg atggcggttt cggcctgcag
     9841 gcgcttcatc agctcgatcg gcactacggt ggcgccggtg gacaggaaac gatagctgga
     9901 aagatcccag tcgcgccagt gggggtgggc cagcatttcc tggaagacgg tgggcggcgc
     9961 cggcatcacg gtgatgcggt tgtgcgcgat gagatcgaga atgctcaccg catcataggc
    10021 ctcgggccag aacacggtgc tgccggtcat cagcccggcc acccagccgg ccttgtagga
    10081 ggcattgtgc gagaaggggc cgaagacgca gtagcggtcc tcggcggtct ggcgcgagat
    10141 gcccgcccag gcccgcccgc cgcccagcga gcggcgatgg ctgaacagcg cccccttggg
    10201 cgcgccggtg gtgcccgagg tgaacatgat gtcgctgggc gtgtcggggc ccaccgcggc
    10261 ctcgcgctcg gccagggcgg ccgggctggt ggcggcggcc agggccagga aggcctgcag
    10321 gctctcctcg cccgcgccca tctcggcggg ggcggcgcgc agcacgatgc gccggcgcag
    10381 ggccggcatc gcctcgccgc gcagcatctg cggataggaa cggcccagga aggtgccgat
    10441 ggcaaacagc cgggtgatcc ccccgcgccg cacgatatcg gccacctcgc cgcccttcag
    10501 ccgggtgttg agcggcacca gcacgccgcc tgcgcgctgg atgcccaccg ccacggccac
    10561 ccattcccac atgttggggg cccagatggc ggcgcgttcg cccgggcggg cgccatcggc
    10621 gatcagcgcg cgggcgacaa gatcggcctg gcggtccagc tcggcatagc tggtggcgcc
    10681 atcctgctgg gagacaagcg cggcccgctc gccccactgg cgggcggcgt ggcgcagaag
    10741 ggcgggaatg gtgtcggtca tcggatcgct gccttgcccc atcatgccga cagatgcgag
    10801 ggcagctggg ccatgaaaat ggccttcacc tcggtgaacc cgtggagccc ttcgacgccg
    10861 ccctcgcgcc ccacgcccga cagcttgaag ccgccatagg ggatcgtata gtcgatcacc
    10921 cggccgttat ggccgaaatt gccggtgcgg atgcgccgcg ccagcgcata ggcgcggtcg
    10981 gcatcgggcg tgaacacccc gccagacagg ccataatcgc tgtcattggc gatggcgatg
    11041 gcttctgcct cgctgtcata ggcgatgatc gtgccgaccg ggccgaagat ctcctcgcgg
    11101 gcgatggtca tgctgttggt ggcctcggca aacagcgtcg gctcgacgaa ccagcccttt
    11161 gtcagatgcg ccgggcggcc gccgccggtg atcacccgcg ccccttcggc cttgcccctc
    11221 tcgatatagc ccagcacccg ctcctgctgg cggccggtga ccagcgggcc catctgcgtt
    11281 gcctcggcaa agggatcgcc cacggccaca tgggccatgg ccgcggccag cgcggcggcc
    11341 acgtcctcct tgcgggcgcg gggcaccagg atgcggctga aggcggcgca ttgctggccg
    11401 cacaggccca ccagattggg catgatgccg gccaccaccg tggcgatatc ggcatcctcc
    11461 agcacgatgg cggccgattt gccgcccagc tcggtggtga tgcgcccgcc ccggccgctg
    11521 acgacagagg caatgtgaag gcccgccgcg gtcgatccgg tgaagctcac cttgtccacc
    11581 gcgcgctggc ggatgagatg gtccgacacg tcgcgatcgg cggtgagcac cgagaagacc
    11641 ccttccggaa cgccggcggc cgccacgcat tcggccagga tcagcgcttc cagcggcgtt
    11701 tccggcgagg gcttggcaat caccgtgcag cccgccgcca gcgccggcgc cactttcagc
    11761 gccagcgtgg ccagcggcgc gttccacggc accaccgcca ccaccacgcc caccggctcg
    11821 cgcacgacga tggcatggcc gcccatcagc ggctggcgca cctcctcgaa gccctgttcg
    11881 gcaaggccgg cgtaataggt gaaatagccg cccatatgcg gcgccacctg ggccgactgg
    11941 gcatagggca tgcccacctg caacgaccag gcgcgggcga aatcatcggc gcgccgggcc
    12001 atttcggctg cgatgcggcg cagatagacg gcccgctcgg cgcccgacag gcgcggccag
    12061 gggccatggt cgaaggcatg gcgggcggcg gccacggcgc ggtccatgtc ggcggtggtg
    12121 gccttggcca cgcggcccac cacttcctcg ctggccggcg agatgatggc gatctgctcg
    12181 gcgccggtgc cggcggtcca gtcgccaccg atgaacagct tgtcggggct ggcaaggttg
    12241 agcagcgtgt cggtcatggg caaggtcctt gttgaatgga agggagcagg ggggcctcag
    12301 gcctccacca ccaccggcag cgagaggata ttggccacgc ccccatccac cggcagcata
    12361 tggccggtga cataggcgct ggcttcggag gcaaggaaca gcaccggccc caccatctgc
    12421 gccggatcgc cgaaatcgcc cagcgcgatg gcgctgcgga agatgcgcgc gcgctcggga
    12481 tggcccacgg tgggggcggc catgtcggtg gcgaagaagc ccggggcaat gccgttgcag
    12541 gtgacgccgt ggggcgccag atcagccgcg atggcgcgcg tcagcccaag cacggcatgt
    12601 ttgctggcgg tgtaggcggc aaggttgggc cgcccggtgg ccgacacata ggagccgaaa
    12661 ttgacgatcc gcccccagcg gcgttccacc atgcctttcg acatttcccg gcacagcaga
    12721 tacatcgagg cgacattcac atcgaacatg gtctgccagt gcggcagggt ggagccatgc
    12781 agcggccccc agccgccgat ggcgccattg ttcaccagaa tgtccaccgc cgcgccatcg
    12841 gcctgcagcc ggtcggtggc ggcctgcacg gcatcaaggt cggccaggtc gaaggcgatg
    12901 gtgcgcacgc tggcgccggc cgcgcgcagt tcatcggcgc tttcctccag ccggccggca
    12961 tcgcgcgcgg ccatcaccag cgcggcgccg gccccggcca gcccgcgcgc gatcgccttg
    13021 cccagcccgc gcgaggcgcc cgttaccatg gctgtgcgcc ccgccagcga gaacagcgcc
    13081 gtcatctgca acctcttcct tggtttatgg gataattggt gcatggccat agcgccggcg
    13141 caagcgcgat tgcgatggca ggccggcacc tatcacaaac gcgattgatc gcccgctggc
    13201 gcatgaaggc cattgccggc gagggggctt ggcggccaat gctgggcgat acaggaacga
    13261 ggtgagtgga tgacggatgc aaggggcccc gcgccggatg cgtgggcgga ttatgtcatc
    13321 gtgggggcag gctctgccgg ctgcgtgctg gccaaccggc tgagcgccgg cgggcggcac
    13381 aatgtggcgg tgctggaagc gggcggcgat gaccgccccg gccgccacct gctgaacccc
    13441 gctcaggccg ccagcacgct gaacatccac ctgcccgccg gcttcacccg catgctgaag
    13501 gacgggcggg tgaactggaa ctatctcacc gaggccgatc ctggcaccgg cgggcgccag
    13561 catgtcttcc cgcgcggcaa ggtgctgggc ggctcgtcct cgatcaacgg catgctgtgg
    13621 gtgcgcggcc tgcccgagga ttacgaacgc tggggccaga tgggcctggc cggctggaac
    13681 tgggccgagg cggagcgcta tttccatcgc atcgagcgcc agcatggtgc cggtgaaagc
    13741 gccggtgagg gcgagggcct gctcgacatt ggcgatgtgc ccatgcagca cccgatgttc
    13801 gatgccatgg cccaggcctt tgccgaggcg ggcgcgccgg tcagcaccga tctcaacggc
    13861 accacccgcg aaggggtggc gcgcgtgcgg gtgaatatcc gcaaggggcg gcgggtgtct
    13921 gccgccacgg cctatctcca tccggcgatg gggcgagcga acctgcgcgt gctcacccat
    13981 gcccatgctg cccgcgtgct gttcgagggg cgccgggcgg tgggcgtgga atatgaacgc
    14041 gccggccagc ggcaggtgat ccgcgcccgg cgcgaggtga tccttgccgg cggcacgatc
    14101 aattcgccgc aattgctgga actgtcgggc attggcgctg tagagcggct ggctgcgctg
    14161 ggcatcgcgg tggtggccaa cagcccccgc gtgggcgaga acctgcagga tcatttcgcc
    14221 gccatggtgc gcgcgcggct gaaacccggc acccccagcc tcaacgaact gtcgcgcggg
    14281 ggggcgctgc tggggcaatt gctgcgcctg gccttcaccg gcaccgggtt gctggcgctg
    14341 ggcggctcca acctcaccgc tttcctgaaa tcggatggtg cgctcgatct gcccgatctc
    14401 cagttcttcg ccagcccggc aacggtggat ttcgaggcgc tggcccgcga tggcgcgatg
    14461 acgatggaac ggcagcccgg catgaccgtg ggcggctatg tgatgcgccc gcaatcggtg
    14521 ggctcgatcc acctgcgcag cgccgatttc cgcgaggcac cggcgatccg gccgaatttc
    14581 ctggcgcatg aggccgattg ccgcgcgcag gtggcctgcc tgcggattgc ccgcaaggtg
    14641 atggccaccc ccgtgctggc gcccttcttc gaccatgagc tgacccccgg cgcggcgctg
    14701 gaaagcgatg cggatctgct cgcctttgcc cgggccagcg gctccaccgg ctatcaccag
    14761 acttccacct gcgcgatggg ggcaggggta gaggcggtgg tgtcgggcgc gctcaaggtg
    14821 aacggggtgg cgggccttcg ggtggccgat gcctcggtga tgccttttgt ggtatcgggc
    14881 aacaccaacg ccgccaccat gatgatcgcc gagaaagccg ccgatctcat ccttgccgag
    14941 gccggttgaa ccgcctccta ttcgcgaaac ggagccaatc cgatggaact gcgcccagat
    15001 cctgcccttg aggccttccg ccaggagatt cgcgccttca tccgccaggt gctgcccgcc
    15061 gacatggccg agcgccagcg ccgcaaggtg gccaacaatg ccgccgtgcc cgatctgctg
    15121 gaatggatgc gcattcttca cagccatggc cgctcggtgc cgcactggcc ggtggaattt
    15181 ggcggctgcg gttggagccc gatgcaattg ttcctcttca acgaggagct ttatgccgcc
    15241 gatgcccccg aattcgactg gggcggcacg catatgctgg gcccggtgct ctacacctat
    15301 ggctcgccct atctgcgcga gcgcttcctg ccgatgatcc gcgagggcgc caccatgtgg
    15361 gcgcagggct tttccgagcc gggctcggga tcggatctgg cctcgctgcg cacctcggcc
    15421 gagctggtgg gcgacaccta tgtcgtcaat ggccagaaga tctggaccag cggggcggcc
    15481 catgccgact ggggcttctt cctggtgcgc accgacagaa gcgcaaagcc ccagcgcggc
    15541 atctccttcc tcatcatccg catggacacg cccggcatca ccgtgcgcca gatcccccag
    15601 atcaacggcg aggcgcatct gtgcgaggtg ttcctcgaca atgtgcaggt gccggccgac
    15661 cagctgatcg gcgagccggg catgggctgg acctatgcca aattcctgct cgaacacgag
    15721 cgcaccacat cgagcttcat ctactggagc aagcgcgaac tggcccgcgc caggcagatc
    15781 gcccgcaccg aacaggccgg cggccggcgc ctggccgatg atccggccat ccgcgcccgc
    15841 ttcgcccggc tggaggccga tctcatcgcg ctggaatggt cggtgctgcg ggtgctggcg
    15901 catgagccgc tggccgtgcc ggaagcggcc gccgcctcgg tgctgaagat caggggcagc
    15961 gccttgcagc aggaagtgac catgctgcag gccgatctct tgggcaccag ggcgctgcgc
    16021 ttcttcgatc cggcgccggc accgccgccc gcgcccacgc ccgattggcc agactatgtg
    16081 cccggccgca ccaccgtggc gctgatcacc cgggcctcca ccatctatgg cggctcgatg
    16141 caggtgcaga agaacattct cgccaaactc gccttcgggc tttgaggggg ctttcgcaga
    16201 ccatggattt tgcccaatcc gatattcaga ccatgctgct cgacagtgcc cagcggctgc
    16261 tggccgccac cgcgggcgtg gaatattggc gcgcgcagcg ccaccagccg ctgggtttcg
    16321 acgaggcgcg ctgggcgcag tttgccgagc tgggctggct ggcgctgccg gtgcccgaag
    16381 cggccggcgg gctgggcgga tcgctggagg atgtggcgct gctgaatgtg gcgctgggcg
    16441 aggcactggc gacagagccc tatgtcagca cggcggtgct ggccgatcat ctgctgaaac
    16501 aggcggcgga tagagcgcag ggccgctacc tgctgggcga gatcgccacc ggcgcgctgc
    16561 gcgtggccct ggcccatcag gaagtggcgc atcaggaggc cgacggaggc acgctggccc
    16621 ggcgcgatgg cacgggcttt gtgctgcagg gcgccaagct gctggcgctg gatgcgccgg
    16681 cggcggggct gctcattgtc tcggcgccaa tcgcggggga ggcggggctg gccctgttcc
    16741 tggtgccggc cgatgccgtg gggctggcgc tggaggccta tccgctgatc gatggcacgc
    16801 gcgcggccga tctgcggctg gaaggcgtgg cgctgccggc atcggccttg ctggtgggcc
    16861 cggagcacgg cgaggcggtg ctggccgagg cgatcgaccg ggcctcggtg gcgctgatgg
    16921 cgcaggcggt gggggcgatg gaagcggcgg tgcagatctg cggccgctat gccaccgaac
    16981 gccgccagtt cggcgtggca atcggctcgt tccaggcgat ccagcacatg ctggccgata
    17041 tgttcgtggc cgcccatcag gcccgctcca tgctctatca cgcgctggcc catgccgagg
    17101 acgaggccgg cgcgcgcggc gcggcgatcg cggcggcgcg catcgttgtc ggcgaggcgg
    17161 ggcagctggt gtcgcgccag ggcatccagc tgcatggcgg ctatggcctg accgatgaat
    17221 atgccatcag ccattatttc cgccggctga tgtgcctgga aaagctctat ggcgatctgg
    17281 cctgccatgt ggagcgtttt ggcgccgccg tgctgggcta gggcgccatg ggccggcagg
    17341 tggcgcccgg gcacggcctg gccgaatgcc gcgcggcgct ggaagcgcat gtgcgccagc
    17401 atggcacggt gccctcgctg gcggggctgg cgcggctgtg gaactatgcc agcaaatcct
    17461 cggcgg
//
LOCUS       DASRYE010000327         8893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_189377,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000327 DASRYE010000000
VERSION     DASRYE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189377"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..3046
                     /locus_tag="VFF98_17000"
     CDS             122..3046
                     /locus_tag="VFF98_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65389.1"
                     /translation="MRLAAHTSLIAITLGWAAAAAAQEAARPAPAGNAADAAADAPAA
                     AEIVVTGSSLKGVAPVGSNLATLARAQIDNTSAQTIQQVLKSAPAVTGLQAPSQGGFG
                     SSDNSGTNAPTIHGLGASASNSTLILMNGHRLPTSGANHMLADPNIIPTIALERVEVL
                     ADGASSVYGSDAVAGVINFITRKNFNGLEADVQKGFADHYNTFSASVLAGKTWDTGSF
                     LAAYSYSYRSSLAASARGYASSVNFAGRGGNNANASNKCASPSITSGGNTYYNTGSAY
                     STEPAGYGSQAGGGCTPTAWDLLPQEVRHTAYMQVRQDVGDKLHLVADFDYSYRKNTQ
                     NISRGTATATIYGQGSTPPSGRSINPYFQSLNLGGSTPTSYTLNFDANQLLGTGAKAI
                     GSAEDFYGHLDAIYDLSTHWQINLGGLIGRDHSQIINSGTLNTAAFALAVNGYTSAAV
                     NGATVANSQTLTSANAFDPFTGQTSSATLAALTDSTTYYSTFQTVRNAYLKASGDLFR
                     LPGGHAKLAVGGELLGYAIDQSHTALNGLGGASSNSTFLRLHYGRNVTSLFGELYLPL
                     VKDSFVKSIDLNISGRYDRYSDFGTTANPKLAANFEPVRGIKLRGNWSRSFVAPALTS
                     IGANSSGQTGESAFTYTTGANNLSTLPGGLAAVPLSLYPGAASIPGAVCNATTCNLTS
                     VTGAVISGGNARLKPQTGKDWSIGFDFTPVQLPGFKLSMTYWNDELRNGITAPQTVYA
                     LTAASLSNLLTLYPAGATVQQIAAAQGSLPQSGATTAAYFIYNYTQNNVLNLNVSGID
                     VEAMYRFDSKIGHFTLDGSFTRKTKFEQWFGADGTHFSVLGTAGFNTTFPSLKMEGRA
                     NIAYDKGPFNAVLGINYEGPYTWWGSGVVNPIVRNAAGVPVGGGDHVGAFTTIDLHAA
                     YTLEHIGALKKANLYVDITNLFDKAPPFVNTVTTNGNVGYDGFSANPLGRVVSIGLRS
                     KF"
     gene            3133..4035
                     /locus_tag="VFF98_17005"
     CDS             3133..4035
                     /locus_tag="VFF98_17005"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZU65390.1"
                     /translation="MPISPSSSAAGGKVAGIGPGRVLAGLVGRGILESRTPWMHECEA
                     DAQGLRMVYALLDFTDRGWSDELLPEVIAAARRIGFAGLNVTFPFKQAVIPLLDELAP
                     GAAAIGAVNTIAFIHGRTIGHNTDVTGFAEAFASGLPDAPVARVLQLGCGGAGSATAH
                     ALLGQLGAQRLILADTDMARAEALARQLADAYGAARVELTDAPASAAASADGLVNATP
                     IGMAKFPGLPIAAEAIRADHWVADIIYFPLETALLKTARALGCRTLNGRGMAVGQAAD
                     AFEVFTGRLPDRARMAASFARFTA"
     regulatory      4071..4261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4566..5582
                     /gene="cobT"
                     /locus_tag="VFF98_17010"
     CDS             4566..5582
                     /gene="cobT"
                     /locus_tag="VFF98_17010"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZU65391.1"
                     /translation="MIRFASPADLTDLLGALPAPDAAAAARAAARQAELTKPAGSLGR
                     LEEVAVFMAGWQGREHPRAERIAVTVFAGNHGVVARGVSAYPAEVTAQMVANFHHGGA
                     AINALAAACGASLSVVPLALDWPTGDITREPALSEADCLDALNAGAGAVASGLDLLIV
                     GEMGIGNTTPAAALAAQAYGGAVEDWVGHGTGIDAATHARKCAAVAGALALHGPFCAS
                     PFETLRRLGGREIVAMAGAMLAARAARVPVLLDGFICCAALAPLAAERPAVLDHCLAG
                     HVSAEVGHARLLERFGKAPLLALNMRLGEGTGAAVAAQVVRSALAAHNGMATFAEAAV
                     AGKL"
     gene            5596..6306
                     /locus_tag="VFF98_17015"
     CDS             5596..6306
                     /locus_tag="VFF98_17015"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZU65392.1"
                     /translation="MKAALLALQFLTRLPLPAVAASERDFAHAIRWFALAGVVVGALV
                     AAAAFAGARRDPWAGALLALIGWVWITGALHLDGLGDIADGAGAAHGNRERLSAVLAD
                     PHIGSFGVVAIGLQLIAKLVALRLFLAGHSPALLVLVPAIARIAPLGWALALPPLHAG
                     LGARMAGGARGWVLALWSLLAIAAAWRWCPALLVAAPAAMLWGLWLRRRIGGISGDGH
                     GAGIELLETAMLLAAACA"
     gene            6320..7780
                     /locus_tag="VFF98_17020"
     CDS             6320..7780
                     /locus_tag="VFF98_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZU65393.1"
                     /translation="MGAIMLQGTGSDVGKSVLVAGLCRALANRGLAVRPFKPQNMSNN
                     AAVTADGGEIGRAQALQAIAARVPAHTDMNPVLLKPQADRTSQLVVHGQVRGTLGSAN
                     FREARRALLGDVLQSFARLRAEADIVVVEGAGSPAEINLRAGDIANMGFARAAGVPVV
                     LVGDIDRGGVIAALAGTRAVLAPEDAAMIRGFVINKFRGDPALFADGYRLIEDFTGWP
                     GLGLVPWLAASARLPSEDAVVLERARPQAGGRRLVACPILPRIANFDDLDPLRAEPGL
                     DVVMVPPGQPLPASAALVVLPGSKATIADMAFLRQEGWDIDILAHHRRGGMVLGICGG
                     YQMLGRAIADPQGIEGPPGAIAGLGLLDVETTLAGTKALREVTGEALGAGFAGYEMHM
                     GVTSGPGAARPFARLADGTADGAISADGRVLGSYCHGLLAAPPLRGALLARLGLASHG
                     ADHAATVDAALDELAEELERHMDIDALIAIAGEPRA"
     gene            7777..8295
                     /gene="cobU"
                     /locus_tag="VFF98_17025"
     CDS             7777..8295
                     /gene="cobU"
                     /locus_tag="VFF98_17025"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZU65394.1"
                     /translation="MTSLLVLGGARSGKSRHAEQWLEALPPGRHGRLAYIATAQVLDD
                     EMAARIAHHRARRNARWHTIEAPIALAEAIGEAAQGADGVLVDCLTLWLTNLILAEAD
                     IAAASAALAAALAAAPVPVALVANEVGLGIVPDNALARRFRDEAGWLNQKLAATASQV
                     VFVAAGLPMVLK"
     gene            8292..>8893
                     /gene="bluB"
                     /locus_tag="VFF98_17030"
     CDS             8292..>8893
                     /gene="bluB"
                     /locus_tag="VFF98_17030"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HZU65395.1"
                     /translation="MSFTAQDARVLEEVLRWRRDVRHFRTTPIDEAVIEAIHAAMELA
                     PSVGNARPWRVVRVESAGARAAVRASFEACTADAADTYTGERRAAYLRLKLAGLDAAP
                     LQLAVFTLADPAEGHGLGRHTLPQTLDQSTAMAIHTLWLAARVRDIGMGMVSILDGAA
                     VERICAVPPSWRFSAYLCLGYPEQEDDTPLLHRLGWQANTR"
BASE COUNT         1300 a         3169 c         2992 g         1432 t
ORIGIN      
        1 cagaccaaag gcgaagacct tgccggattg acgcgcctct aagttgtatg taccagttag
       61 ttcataaaca ccggcacccg gccggaatcg ccctgggagg ggcaacgcca tgaacggaaa
      121 gatgcggctt gccgcacaca cctcgcttat cgccatcacc ctgggctggg ccgctgccgc
      181 tgccgcgcag gaggccgccc gccccgcgcc cgccggcaat gccgccgatg ccgcagccga
      241 tgcccccgcc gctgccgaga tcgtcgtcac cggctccagc ctgaagggcg tggcgccggt
      301 cggctcgaac ctggccaccc tggcccgcgc gcagatcgac aacacctcgg cgcagaccat
      361 ccagcaggtg ctgaaatcgg caccggccgt gaccggcctg caggccccca gccagggcgg
      421 cttcggctcg tccgacaatt ccggcaccaa tgcgcccacc atccatggcc tgggcgcctc
      481 ggcctccaat tccaccctca tcctgatgaa cggccaccgc ctgcccacct cgggcgccaa
      541 ccacatgctg gccgatccca acatcatccc caccatcgcg ctggaacggg tggaagtgct
      601 ggccgatggc gcttcctcgg tctatggctc ggatgcggtg gcgggcgtga tcaacttcat
      661 cacccgcaag aacttcaatg gcctggaagc cgatgtgcag aagggctttg ccgatcatta
      721 caacaccttc agcgcctctg tgctggccgg caaaacctgg gataccggct ccttcctcgc
      781 cgcctattcc tattcctatc gctccagcct ggcggcctcg gcgcgcggct atgccagcag
      841 cgtgaacttt gccgggcgcg gcggcaacaa tgccaatgcc agcaacaaat gcgccagccc
      901 ctcgatcacc tcgggcggca acacctatta caacaccggc tctgcctaca gcaccgagcc
      961 ggccggctat ggctcgcagg ccggcggcgg ctgcacgccc acggcctggg atctgctgcc
     1021 gcaagaagtg cgccacaccg cctatatgca agtgcgccag gatgtgggcg acaagctgca
     1081 cctggtggcc gatttcgact attcctaccg caagaacacc cagaacatct cgcgcggcac
     1141 cgccacggcc accatctatg gccagggttc cacgccgcct tcgggccgct cgatcaaccc
     1201 ctatttccag tcgctgaacc tgggcggcag cacgcccacc tcctatacgc tgaacttcga
     1261 tgccaaccag ctgctgggca ccggcgccaa ggccatcggc agcgccgagg atttctacgg
     1321 ccatctcgat gcaatctatg atctcagcac gcattggcag atcaatctgg gcggcctgat
     1381 cgggcgcgat cattcgcaga tcatcaattc gggcacgctg aacaccgccg cctttgcgct
     1441 ggccgtcaac ggctacacct cggccgcggt gaacggcgcc acggtggcca acagccagac
     1501 cctgaccagc gccaacgcct tcgatccctt caccggccag acctcttcgg caacgctggc
     1561 agcgctcacc gacagcacca cctattattc caccttccag accgtgcgga acgcctatct
     1621 gaaggccagc ggcgatctct tccgcctgcc cggcggccat gccaagctgg cggtgggcgg
     1681 cgaattgctg ggctatgcga tcgaccagtc gcacaccgcg ctcaatggcc tgggcggcgc
     1741 cagcagcaat tccaccttcc tgcgcctgca ctatggccgc aacgtcacct cgctgttcgg
     1801 cgagctgtat ctgccgctgg tgaaggacag cttcgtcaag tcgatcgatc tcaacatctc
     1861 gggccgttat gatcgctaca gcgatttcgg caccaccgcc aaccccaagc tcgccgccaa
     1921 tttcgagccg gtgcgcggga tcaagctgcg cggcaactgg tcgcgctctt tcgtggcgcc
     1981 ggcgctcacc agcattggcg ccaattcctc tggccaaacg ggcgaatccg ccttcaccta
     2041 caccaccggc gccaacaacc tctccaccct gccgggcggc ctcgctgccg tgccgctctc
     2101 gctctatccc ggcgctgcca gcattccggg cgctgtgtgc aatgccacca cctgcaacct
     2161 caccagcgtg acgggcgcgg tgatctcggg cggcaatgcc cgcctcaagc cgcaaaccgg
     2221 caaggactgg agcatcggct tcgatttcac ccccgtgcag ctgcccggct tcaagctgag
     2281 catgacctac tggaacgacg aactgcgcaa cggcatcacc gccccgcaaa ccgtctatgc
     2341 gttgaccgcg gccagcctgt cgaacctgct gacgctgtat ccggccggcg ccaccgtgca
     2401 gcagatcgcc gccgcgcaag gcagcctgcc gcaatcgggc gccaccaccg ctgcctattt
     2461 catctacaac tacacccaga acaacgtgct caacctcaat gtctcgggca tcgatgtgga
     2521 agcgatgtat cgcttcgaca gcaagatcgg ccatttcacg ctggatggct ccttcacccg
     2581 caagaccaag ttcgaacagt ggttcggcgc cgatggcacc catttcagcg tgctgggcac
     2641 ggccggtttc aacaccacct tcccctcgct gaagatggaa gggcgcgcca acatcgccta
     2701 cgacaagggc cccttcaacg cggtgctggg catcaattat gaaggcccct acacctggtg
     2761 gggctcgggg gtggtcaatc ccattgtccg caatgccgcc ggcgtgccgg tgggcggcgg
     2821 cgatcatgtc ggcgccttca ccaccatcga cctgcatgcg gcctacacgc tggaacatat
     2881 cggcgcgctg aagaaggcca atctctacgt cgatatcacc aacctgttcg acaaggcccc
     2941 gcccttcgtc aacaccgtca ccaccaacgg caatgtcggc tacgacgggt tcagcgccaa
     3001 tccgctgggc cgggtcgtca gcattggcct gcgctcgaag ttctgatcat ccaccctgcc
     3061 ttgccgcccc gaagccctct ctccttcggg gcggatcttt tccccttcct gcccagcgga
     3121 gccgcccagc ccatgcccat ttctccttcc tcttctgcgg ccggtggcaa ggtggcgggc
     3181 attggcccgg ggcgcgtgct ggcagggctg gtcggccggg gcatccttga aagccgcacg
     3241 ccctggatgc atgagtgcga ggccgatgcc caggggctgc gcatggtcta tgccctgctc
     3301 gatttcaccg accgcggctg gagcgacgag ctgctgcccg aggtgatcgc ggcggccagg
     3361 cgcatcggct ttgccgggct caatgtcacc tttccgttca agcaggcggt gatcccgctg
     3421 ctcgatgagc tggcgccggg ggcggcggcc atcggcgctg tcaacaccat cgccttcatc
     3481 cacggccgca ccattggcca caacaccgat gtgacaggct ttgccgaggc ctttgccagc
     3541 ggcctgccgg acgcccctgt ggcgcgcgtg ctgcagctgg gctgcggcgg ggcgggttcg
     3601 gccacggccc atgcgctgct tgggcagctg ggcgcccaaa ggctgatcct ggccgacacc
     3661 gacatggccc gcgccgaggc gctggcccgc cagctggccg atgcctatgg cgctgcccgc
     3721 gtggagctga ccgacgcgcc cgccagcgcc gctgccagcg ccgatggcct ggtcaacgcc
     3781 acgcccatcg gcatggccaa attccccggc ctgccgatcg cggccgaggc catccgcgcc
     3841 gatcactggg tggccgacat catctatttc ccgctcgaaa ccgccttgct gaaaacggca
     3901 cgggccctcg gttgtcgcac gctgaacggg cggggaatgg cggtggggca ggcggccgat
     3961 gccttcgagg tgttcaccgg ccgcctgccc gaccgcgccc gcatggccgc cagcttcgcg
     4021 cgcttcacag cctgaacgca gggggcttgc cacggcgccc ccgccgcgat aaccagccag
     4081 ccgcgacagg ttccccgccg ggggatcaaa agggaagccg ggtgcaagtc ctggcgctgc
     4141 ccctgcaact gtgagcggcg agccatccgg ccacatgcca ttgggagccc cctcccgaga
     4201 aggcggccgg caaggcgttg acccgcaagc caggagacct gcctgccgca gtcgttcttg
     4261 gtgccgacag ggtgtgcggt gcctggcggg gtttcccctg atcgaacgac agccgtgccg
     4321 gcgtgggccc tgtggcctgc gccggatgtc gcctgaatgt tgccagaggg accagacatg
     4381 cccccgccac cctgctgccc gccgccctgg ccatggccct tctgcgcgcc ggccgccgcc
     4441 gctgctggcg caagcggcct tgtccgcccg gcgggcgccc cctatgcagt tgggccagat
     4501 gggctaaagg gcctgcccgt ctgccgcgaa ccccaccgcg ttccacccga aggagccgtt
     4561 gccccatgat ccgctttgcc agccccgccg atctcaccga tctgcttggc gccctgcccg
     4621 cgcccgatgc cgccgccgcc gcccgcgccg ccgcccgcca ggcagagctg accaaacctg
     4681 ccggatcgct gggccggctg gaggaagtgg cggtgttcat ggccggctgg cagggccgcg
     4741 aacacccccg cgccgaacgg atcgcggtca ccgtgtttgc cggcaaccat ggcgtggtgg
     4801 cgcgcggcgt cagcgcctat ccggccgagg tgaccgccca gatggtggcc aatttccacc
     4861 acggcggggc ggcgatcaat gcgctggcgg cagcctgcgg ggccagcctc tcggtggtgc
     4921 cgctggcgct ggactggccg accggcgaca tcacccgcga accggcgctg agcgaggccg
     4981 actgcctcga tgcgctgaac gcgggcgccg gcgctgtggc cagcgggctg gatctgctga
     5041 tcgtcggcga gatgggcatt ggcaacacca cgccggccgc cgcccttgcc gcccaggcct
     5101 atggcggcgc cgtggaagac tgggtgggcc atggcaccgg catcgatgcc gccacccatg
     5161 cccgcaaatg cgcggcagtg gctggcgcgc tggcgctgca tgggccgttc tgcgcctcgc
     5221 ctttcgaaac gctgcggcgg ctgggcgggc gcgagatcgt cgccatggcg ggcgccatgc
     5281 tggccgcgcg ggccgcgcgc gtgccggtgc tgctggatgg cttcatctgc tgcgcagcgc
     5341 tggccccact ggcggcggaa cggccggcgg tgctcgatca ttgtctggcc ggccatgtct
     5401 cggccgaagt gggccatgcc cgcctgctgg aacgcttcgg caaggcgccg ctgctggcgc
     5461 tgaacatgcg gctgggcgaa ggcaccgggg cggcggtggc ggcgcaggtc gtgcgctcgg
     5521 cgctggccgc gcataatggc atggccacct ttgccgaggc ggccgtggcc ggcaagctgt
     5581 gaaggccgac gggcagtgaa ggcagcgctg ctggcccttc agttcctcac gcgcctgccg
     5641 ctgccggccg tcgccgccag cgagcgggat tttgcccatg ccatccgctg gttcgcgctg
     5701 gcgggcgtgg tggtgggcgc gctggtggca gctgcggcct ttgccggcgc gcggcgcgat
     5761 ccgtgggcgg gcgcgctgct ggcgctgatc ggctgggtgt ggatcaccgg cgccctccat
     5821 ctggatgggc tgggcgatat cgccgatggc gccggcgctg cccatggcaa ccgcgagcgc
     5881 ctctcggccg tgctggccga tccgcatatc ggcagctttg gcgtggtggc gatcggcctg
     5941 cagctgatcg ccaagctggt ggcgctcagg ctgtttctgg ccggccacag ccctgccctg
     6001 ctggtgctgg tgccggccat cgcgcggatc gccccgctgg gctgggcgct ggcgctgccg
     6061 ccgctgcatg cggggctggg cgcgcgcatg gcgggcggcg cgcgcggctg ggtgctggcc
     6121 ttgtggagcc tgctggccat tgccgctgcc tggcgctggt gcccggcgct gctggtggcg
     6181 gcgccggcgg caatgctgtg gggcctgtgg ctcaggcggc gcatcggcgg catctcgggc
     6241 gatggccatg gcgcggggat agaactgctg gaaacggcca tgctgcttgc cgcggcctgc
     6301 gcctgaagga gaagccgcaa tgggcgcgat catgctgcag ggcaccggct ctgatgtcgg
     6361 caaatcggtg ctggtggccg ggctgtgccg ggcgctggcc aatcgcgggc tggcggtgcg
     6421 cccgttcaag ccgcagaaca tgtcgaacaa tgccgccgtc acggccgatg gcggcgagat
     6481 cggccgcgcc caggcgctgc aggcgatcgc cgcgcgggtg cccgcccaca ccgacatgaa
     6541 ccccgtgctg ctgaagcccc aggccgatcg cacctcgcag ctggtggtgc atgggcaggt
     6601 gcgcggcacg ctgggctctg ccaatttccg cgaggcgcgc cgcgcgctcc tcggcgatgt
     6661 gctgcaaagc tttgcccgcc tgcgggccga ggctgatatc gtggtggtgg aaggcgccgg
     6721 cagcccggcc gagatcaacc tgcgcgcggg cgatatcgcc aacatgggct ttgcccgcgc
     6781 ggccggcgtg ccggtggtgc tcgtgggcga tatcgacagg ggcggggtga tcgccgccct
     6841 ggccggcacc agggcggtgc tggcgcccga ggatgcggcg atgatccgtg gctttgtcat
     6901 caacaagttc cgcggcgatc cggcgctgtt tgccgatggc tatcgcctga tcgaggattt
     6961 caccggctgg cccggtctgg ggctggtgcc ctggctggcg gccagcgcgc gcctgcccag
     7021 cgaggatgcc gtggtgctgg aacgcgcccg cccgcaggca ggggggcgcc ggctggtggc
     7081 ctgcccgatc ctgccgcgca tcgccaattt cgatgatctc gaccccttgc gcgcagagcc
     7141 cggcctcgat gtggtgatgg tgccccccgg ccagcccctg ccggccagcg ccgcgctggt
     7201 ggtgctgccc ggatcgaagg cgaccatcgc cgacatggcc ttcctgcgcc aagagggctg
     7261 ggatatcgac atcctcgccc atcaccggcg cgggggcatg gtgctgggca tttgcggggg
     7321 ctatcagatg ctgggccgcg ccattgccga tccgcaggga atagaagggc cgccaggcgc
     7381 cattgccggc cttggcctgc tggatgtgga gaccacgctg gccggcacca aggccctgcg
     7441 cgaagtgacc ggcgaggcgc tgggcgcggg ctttgccggc tatgagatgc atatgggcgt
     7501 cacctctggc cccggcgcgg cccgcccctt cgcccggctg gccgatggca ccgccgatgg
     7561 cgccatcagt gcggatgggc gggtgctggg cagctattgc cacgggctgc tggcagcgcc
     7621 gcccttgcgg ggcgccttgc tggcgcggct gggccttgcc agccatggcg ccgatcatgc
     7681 cgccacggtg gatgcggcgc tggatgaact ggcagaagag ctggaacgac atatggacat
     7741 agacgcgctg atcgccatcg ccggggagcc ccgcgcatga ccagcctgct ggtgctgggc
     7801 ggcgcccgtt cgggcaagag ccgccatgcc gaacagtggc tggaagcgct gccgcccggc
     7861 cgccatggcc ggctggccta tatcgccacc gcgcaggtgc tggatgacga gatggccgcc
     7921 cgcatcgccc accaccgcgc ccgccgcaat gcccgctggc acacgataga ggcgcccatc
     7981 gccttggccg aggcgatcgg cgaggcggct caaggcgccg atggcgtgct ggtcgattgc
     8041 ctgaccctgt ggctgactaa cctgatcctc gccgaggccg atattgccgc tgccagcgcc
     8101 gcgctggccg ccgcgctggc cgccgccccc gtgccggtgg cgctggtggc caatgaggtg
     8161 ggccttggca tcgtgcccga caatgcgctg gcccggcgtt tccgcgacga ggcgggctgg
     8221 ctcaaccaga agctggcggc cacggccagc caggtggtgt tcgtggccgc cggcctgccg
     8281 atggtgctga aatgagcttt accgcgcagg atgcccgcgt gctggaagag gtgctgcgct
     8341 ggcggcgcga tgtgcgccat ttccgcacca cgccgattga cgaggcggtg atcgaggcga
     8401 tccacgcggc gatggagctg gccccttctg tgggcaatgc gcggccctgg cgggtggtgc
     8461 gggtggagag cgccggcgcg cgcgcggcgg tgcgggccag tttcgaggcc tgcaccgccg
     8521 acgccgccga cacctacacc ggcgagcgcc gcgccgcata tctgcggctg aagctggccg
     8581 ggctggatgc cgcgcccttg cagctggcgg tcttcaccct tgccgatccg gccgagggcc
     8641 atggccttgg ccgccacacc ctgccgcaaa cgctggacca atccaccgcc atggcgatcc
     8701 acacgctgtg gctggcggca agggtgcgcg atattggcat gggcatggtt tccatccttg
     8761 atggcgcggc ggtggagcgg atctgcgccg tgcccccgag ctggcgcttc tccgcttacc
     8821 tgtgcctggg ctatcccgag caggaggacg atacgccgct gctccaccgg ctgggctggc
     8881 aggccaatac ccg
//
LOCUS       DASRYE010000328         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_173100,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000328 DASRYE010000000
VERSION     DASRYE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173100"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFF98_17035"
     CDS             <1..756
                     /locus_tag="VFF98_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65396.1"
                     /translation="IRRPPLSEKDMWGMTIFDQLACRIAFRQMRYQGDFTPQSEQPTL
                     HYPGNLGGMNWGGATLFRPLDYLIVNDIRVPVVFRLIPRRADGRADIFGSLAKYPQYG
                     TPYLASGQPFFSALGIPCSSPPFGTMTAIDLRRRQIVWQVPIGTTRDSAPFGFRLGVP
                     FALGMPTRGGSLATAGGLVFFGSATDRVMRALDVRNGRILWEGGLPVGANATPMTYVS
                     PRSGRQYIVQTASGSMGTPGAGDYVVAWALDRQ"
     gene            907..1740
                     /locus_tag="VFF98_17040"
     CDS             907..1740
                     /locus_tag="VFF98_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZU65397.1"
                     /translation="MSYKFDKTVPYRTHLEQYRETWADLAHLRREDGILEVRFHWNGG
                     PWRWNEAMHTALVPFCADISQDPENECIIITGTGDSFLDEFDAETVLASDTDHSRLVT
                     YDWWWRAATRVPGAIIDIPVPVVSAINGAVSIHPEIVLLADVVIASEETVIIDRHYQE
                     AGVAPTDGINILYEKFFGPNLARSALFLGTEISAAQALQFGVFAEVLPAEQVLERAWE
                     IARTKIMTASRIHRRMTREALIQPYREAFARSIRASLAHECYAAQSTPGEIPHGKMLK
                     K"
     gene            1875..2939
                     /locus_tag="VFF98_17045"
     CDS             1875..2939
                     /locus_tag="VFF98_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65398.1"
                     /translation="MRFVTFEIPSLIGPIRRAGVLGSADTIIDISAAREALLRDRGVS
                     GPARRAQAECPSSLLAFIEAGAPALDALGEALAFVEGHGERVLGGLTVSFASADVDLL
                     TPLPVPRSIRNFSLSEQHMLDSIETMKKKIGGMEPSLVRIPPEWYNLPAYYKTTVMEV
                     YGPNDLIPWPELTGKFDYELELAAVIGKRGRHVAVEEAQPYIFGFTLYTDWSMRDFQQ
                     REMSINMGPGLCKDFASSLGPCIATPDEFDVVNARLTARIDGQLWTDTTSRLRCSFEE
                     LIAYVTQTQTIVPGDIFTSGTVPGGSGSEQDRWMPEGAVVELEAEGIGVLRNKVGTRG
                     VAVPLPRSQQPWVSSQRQDG"
     gene            3081..4268
                     /locus_tag="VFF98_17050"
     CDS             3081..4268
                     /locus_tag="VFF98_17050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65399.1"
                     /translation="MVSYIDASAVVSTGVALVMFQHAFRLGSLQIGYLSSSLGLLVAL
                     GALLGGWLSDNYGRRRVFNLTLLGLVLGCTVMALAQGFGVLLAGVILLGFFVGASLPA
                     SLALVGEFAPPGARGRMLTFSQVLWNVGILVTQLIIALVSDIGVAGGRLLFVHLIVMS
                     VLVAAMHWRLPESPVWAAGHAGPRPGGASQLGFSQFAELTSKPYLALFAGLAAFYGLA
                     QVAASIKAQFLGFMFVNVAGSTLRLYSAAYMSVMVVGLIAMMLFMRVVDGRNRLGWFV
                     SGGLAFIASFAVPAVLGVTVPSLFVSSLLSGFGGALAFEGIMKVWAQEFFPASLLGTA
                     QGSVIAFGRILAALMAIWAPTLLMLNARGFFAFLTGVMALAMAIGLCLALHSERSRRS
                     ARG"
     gene            complement(4298..5086)
                     /locus_tag="VFF98_17055"
     CDS             complement(4298..5086)
                     /locus_tag="VFF98_17055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZU65400.1"
                     /translation="MTNTASRAPANGKPGRRPRTRPAQAEPQADSTEPRKPGPRGAAT
                     GDTATGTDELCARLREDIVRCVLPPHARLKFGDLQQRYGAGIGSLREALMQLTAEGMV
                     ISESFRGFCVAPISLDDLQDLTELRVEFENRALRASILLGDDQWEARIVAALHMLNKI
                     THIGPDSSDSEMAEWEERHGRFHEALLSACPSRRVMQIRKTLYDQGRRYRNLALARSG
                     AVEVHAYQHQELANLVLARDADAACALMTSHILEAANNAGRLVD"
     gene            complement(5092..5925)
                     /locus_tag="VFF98_17060"
     CDS             complement(5092..5925)
                     /locus_tag="VFF98_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65401.1"
                     /translation="MRQAEQPGWNRRVPLAALGSALLAWGTAAQAAPGADHLMLMFNA
                     PAGDLAAYQNWYDHIHAPAILAVPGFVDAQRAEAAPPGPLPDMAPPSPFLIRYRLRSA
                     DLAATFAAVPKPDPAHPAPIAPGGFALVFDALGTERRGAGALVLPHLGPPATFDLYVL
                     ESPREGEESAFNQWYDQAQLARMLATPGIVSARRYVRSPIQRDRTRPSPRYLAAYRIV
                     SNRIDLVLAGLARQGALQPSGETVDAGASMRFLYRLCGPLINHSGQLPAPARADSGRK
                     E"
     gene            6245..>6631
                     /locus_tag="VFF98_17065"
     CDS             6245..>6631
                     /locus_tag="VFF98_17065"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZU65402.1"
                     /translation="MATVLAQASAAQAQVAPAAQVPAAQLPAAGAPAADMPAASDIIV
                     TAERRSVNLQKAALSISALTGDSLQVKGATSVADAIKATPSVVIQQSTGGYSAATIIG
                     GGGPPNMSIRGLGTDGFNKQPSVRVYQ"
BASE COUNT         1029 a         2050 c         2317 g         1235 t
ORIGIN      
        1 atccgccgcc cgccgctgag cgaaaaggac atgtggggca tgacgatctt cgatcagctg
       61 gcctgccgca tcgccttccg ccagatgcgc tatcagggtg atttcacccc gcaatcggaa
      121 cagccgacct tgcattatcc gggcaatctg ggcggcatga actggggcgg cgccacgctt
      181 ttccggccgc tcgattatct catcgtcaac gatatccggg tgccggtggt gttccgcctg
      241 atcccgcgcc gggctgatgg ccgggcggat atcttcggca gcctggcgaa atatccgcaa
      301 tatggcacgc cctatctggc atcgggccag ccgttcttct cggcgctggg cattccgtgc
      361 agcagcccgc ctttcggaac gatgacggcg atagacctgc gccggcgcca gatcgtctgg
      421 caggtgccaa tcggcacgac gcgggattca gcgcctttcg gttttcgcct tggcgtgccc
      481 ttcgcgctgg gcatgcccac gcgcggcgga tcgctggcaa cagctggcgg ccttgtcttc
      541 ttcggcagcg cgacagaccg ggtgatgcgg gcgctcgatg tcagaaacgg ccggatcctg
      601 tgggagggcg gcttgccggt gggggccaac gccacgccga tgacctatgt ctcgccgcgc
      661 agtggccgcc agtacatcgt ccagacggca tcgggcagca tgggcacgcc cggggccggg
      721 gattatgtcg tggcctgggc tctcgatcgc caatgaagca ttgcacaggc gccggctcat
      781 ccgcttggat gaggcagcgc ctgtgcggat gtgcaacaag atgcccgaca ggaagcgagc
      841 catcgcaggt gggaaaggtg ccgacgtgcg cggttgccag cacgagctga aaacggaaag
      901 gaagatatgt cttacaaatt cgacaagacg gtcccctatc gcacgcatct ggagcaatat
      961 cgggagacct gggccgatct ggcgcatctc aggcgggaag atggcattct ggaagtgcgc
     1021 tttcactgga atggcgggcc ctggcgttgg aacgaggcga tgcatacggc gctggtgccg
     1081 ttctgcgccg atatttccca ggatccggag aatgaatgca tcatcatcac cggcaccggc
     1141 gattccttcc tggatgagtt cgatgccgaa accgtgctgg cctccgatac cgaccattcc
     1201 cggctggtga cctacgactg gtggtggcgc gcggccacgc gcgttcccgg cgcgatcatc
     1261 gacattcccg tcccggtcgt ttccgcgatc aatggcgcgg tgtcgatcca cccggaaatc
     1321 gtgctgctgg ccgatgtggt gatcgccagc gaggagacgg tgatcatcga tcgccattac
     1381 caggaagcgg gcgtggcgcc gacagacggc atcaatatcc tctatgagaa gttcttcggg
     1441 ccgaatctgg cccgctcggc gctgtttctg ggcaccgaga tcagcgcggc ccaggcgctg
     1501 cagttcggcg tgttcgccga ggtgctgccg gccgaacagg tgctggagcg ggcctgggag
     1561 atcgcccgca ccaagatcat gaccgcctcg cgcatccacc gccggatgac gcgtgaagcg
     1621 ctgatccagc cctatcgcga agccttcgcc aggtcgatcc gggccagcct tgcccatgaa
     1681 tgctatgcgg cacaatcgac ccccggtgaa attccccatg ggaaaatgct gaagaaataa
     1741 gcggcgggct ttcgcctgcc ccccttgcgc atcggggatg atcgcgcccg attgcccggc
     1801 ggcctctgcc ggcggcatgc gcgcgcctgt gcctgttgtg ctttcccctg cgccattgaa
     1861 gaggaccaac cgccatgcgt ttcgtgactt ttgaaattcc ctcgctcatc gggccgatca
     1921 ggcgggcagg cgtgctgggc agcgccgata ccatcatcga tatcagcgcc gcgcgcgagg
     1981 ccctgctgcg tgatcgcggt gtgtcggggc cggcgcgccg ggcgcaggcc gaatgcccct
     2041 ccagcctgct ggcgttcatc gaggcagggg cgccggcgct cgatgcgctg ggcgaggcgc
     2101 tggcctttgt ggaaggccat ggcgagcggg tgctgggcgg gctcaccgtc tcctttgcca
     2161 gcgccgatgt cgacctgctg accccgctgc cggtgccgcg ctcgatccgc aatttttccc
     2221 tgtccgagca gcatatgctg gattcgattg aaacgatgaa gaaaaagatc ggcgggatgg
     2281 agccttcgct ggtgcgtatt ccgcccgaat ggtacaattt gccggcctat tacaagacca
     2341 cggtgatgga ggtctatggc cccaacgatc tgatcccctg gccagagctg accggcaagt
     2401 tcgactacga gctggagctg gccgcggtga tcggcaagcg cgggcgccat gtcgcggtgg
     2461 aagaggcgca gccttatatc ttcggcttca ccctctacac cgactggagc atgcgcgatt
     2521 tccagcagcg cgaaatgagc atcaacatgg ggccgggcct gtgcaaggat ttcgccagct
     2581 cgctggggcc gtgcatcgcc acgccggacg agttcgatgt cgtgaacgcc cgcctcaccg
     2641 cgcggatcga cggccagctg tggaccgaca cgacttcgcg gctgcgctgc agctttgaag
     2701 agctgatcgc ctatgtcacc cagacgcaga cgatcgtgcc cggcgacatt ttcacttccg
     2761 gcacggtgcc gggcggcagc ggcagcgaac aggaccgctg gatgcccgaa ggggccgttg
     2821 tggagctgga ggccgagggc attggcgtgc tgcgcaacaa ggtgggcacg cgcggggttg
     2881 cggtgcccct gcccagatcg cagcagccat gggtttcctc gcagcgccag gacggataga
     2941 caggtcttcg aaaaaatcgt ggaaaataac gggatggccg tgcccgccgg gcacaggcca
     3001 tccgtcggga gagcaatgca atggctgacg cgcaggccct gccaacgtgg cgggattggt
     3061 atgttgctgc cttggcgggc atggtgtcct atatcgatgc aagcgctgtg gtgtcgacgg
     3121 gcgtggccct ggtgatgttt cagcatgcct tccggctggg cagcctgcag atcggctatc
     3181 tgtcgtcctc gctgggcctg ctggtggcgc tgggggcgct gctggggggc tggctgagcg
     3241 acaattacgg ccgcaggcgg gtgttcaacc tcaccttgct cgggctggtt ctgggctgca
     3301 cggtgatggc cctggcgcaa ggctttggcg tgctgctggc gggcgtgatc ctgctgggtt
     3361 tttttgtcgg tgcctcgctg cctgcctcgc tggcgctggt gggggaattc gcgccgcccg
     3421 gcgcgcgcgg ccggatgctc accttttcgc aggtcctgtg gaatgtcggc attctggtga
     3481 cccagctgat cattgcgctg gtgtcggata tcggcgtggc gggggggcgg ctgctgtttg
     3541 tccatctgat cgtcatgtcg gtgctggtgg ccgcgatgca ctggcgcttg ccggaatccc
     3601 cggtctgggc ggcagggcat gccggcccgc ggccgggcgg cgccagccag ctggggtttt
     3661 cgcaattcgc agagctgacc agcaagccct atctggccct gttcgccggg ctggccgcct
     3721 tttacgggct ggcgcaggtg gccgccagca tcaaggccca gttccttggc ttcatgttcg
     3781 tgaatgtggc cggcagcacg ctgcggctct attctgcggc ctatatgtcg gtgatggtgg
     3841 tcgggctgat tgccatgatg ctgttcatgc gggtggtcga tgggcgcaac cggctgggct
     3901 ggtttgtttc gggcgggctg gctttcattg ccagtttcgc cgtgccggcg gtgctgggcg
     3961 tgaccgtgcc gagcctgttc gtcagttcgc tgctgtccgg ttttggcggg gcgctggcct
     4021 tcgaggggat catgaaggtg tgggcgcagg aattcttccc ggcctcgctg ctgggcaccg
     4081 cgcaaggctc ggtgattgcg ttcgggcgca ttctggcggc gctcatggcg atctgggcgc
     4141 ccacgctgct gatgctcaat gcgcggggct ttttcgcctt tctcaccggg gtgatggcgc
     4201 tggccatggc gatcggcctg tgccttgccc tccattccga acgcagccgc cggtccgcgc
     4261 ggggctgacg gcgcagtgcc gggccggccg ggcggttcta gtccaccagc cggccggcat
     4321 tgttggccgc ttccagtata tggctggtca tcagcgcgca ggccgcatcg gcatcgcgcg
     4381 ccagcaccag attggccagt tcctggtgtt gataggcatg cacttccacc gcgccgcttc
     4441 tggccagggc caggttgcga taacgccggc cctgatcgta gagcgtcttg cggatctgca
     4501 tcacgcgccg cgacgggcag gccgaaagca gggcctcgtg gaagcggcca tggcgctctt
     4561 cccattcggc catttcgctg tcgctggaat ccggcccgat atgggtgatc ttgttcagca
     4621 tgtgcagcgc ggccacgatg cgcgcttccc actggtcgtc acccagcagg atcgaggcgc
     4681 gcaaggcccg gttttcgaac tcgacgcgca gttcggtcag gtcctgcaga tcgtcgagcg
     4741 agatcggcgc gacgcagaag ccgcgaaagg attcgctgat caccatcccc tcggccgtga
     4801 gctgcatcag cgcctcgcgc agggagccga tgcccgcgcc atagcgctgc tgcagatccc
     4861 cgaacttcag ccgggcatgg ggcggcagca cgcagcgcac gatgtcttcg cgcagcctgg
     4921 cgcataattc gtcggtgccg gttgccgtat cgccggtggc cgcgccgcgc gggccgggct
     4981 ttctgggttc tgtgctgtcg gcctgtggtt cggcctgggc ggggcgggtt ctgggccggc
     5041 gcccgggttt gccgttggcc ggtgccctgc tggcggtatt ggtcaagtgc gttattcctt
     5101 cctgccgctg tccgcgcgcg ccggggcagg caactggcca gagtggttga tgagggggcc
     5161 gcagagccga tagaggaagc gcatggaggc gcccgcgtca acggtttcgc ccgatggctg
     5221 cagggcgcct tgccgcgcca gcccggccag caccagatcg atccggttgc tgacgatgcg
     5281 ataggcggcc agatagcggg ggctgggcct ggtgcgatcg cgctggatgg ggctgcggac
     5341 atagcgccgc gcgctgacga tgccgggcgt ggccagcatg cgggccagct gggcctgatc
     5401 gtaccactgg ttgaaggcgc tctcctcgcc ttcgcgcggg ctttccagca catagagatc
     5461 gaaggtggcg ggcgggccga gatgcggcaa caccagggcg cccgcccctc gccgttcggt
     5521 gcccagggca tcgaagacca gggcgaaacc gcccggcgcg atgggtgccg gatgggcggg
     5581 atcaggcttg ggcactgcgg cgaaggtggc ggccagatca gcgctgcgca gccggtagcg
     5641 gatcaggaag gggctgggcg gtgccatgtc gggcaagggg cccggcggtg cggcctcggc
     5701 ccgctgggca tcgacaaagc cgggaaccgc cagaatcgcc ggcgcgtgga tatggtcgta
     5761 ccagttctga taggcggcca gatcgccggc gggggcgttg aacatcagca tcaggtgatc
     5821 ggcccccggc gcggcctgcg ccgccgtgcc ccaggccagc agcgcgctgc ccagcgcggc
     5881 gagggggacg cggcggttcc agccgggttg ctcagcctgt cgcattgcct ctccttttgt
     5941 gcattatttc tataaaattg aacatttgaa ttatgttcga aaataactag aaatcaagag
     6001 cgatgtcggg cggggccgcg cacaggtgcc ctggcgggcc cggcgcgagg ctcatctgtt
     6061 cagatgaagc agccccgaag ggcggggcga tagaaggaga acagcacggc gcggccagat
     6121 cggccggggg ccgtcggaac gggtacctgg cgcagatatc gcccgttggc gggtgggaaa
     6181 agtccggggc ccgcataagg gagagcgata catgcaactg ataaggcccg tgggtttggc
     6241 cgccatggca acggttctgg ctcaggcttc ggcggcgcag gcacaggtgg cgcctgctgc
     6301 ccaagtgccc gctgcccagc tacctgccgc cggtgcgccc gccgccgata tgcctgcggc
     6361 aagcgatatc atcgtcacgg ccgaaaggcg cagcgtcaac ctgcagaagg cggccctgtc
     6421 gatcagcgcg ctgaccggag acagcctgca ggtcaaaggc gcaaccagcg ttgccgatgc
     6481 catcaaggcc acccccagcg tggtgatcca gcaatccacc ggcggctatt cggcggccac
     6541 gatcattggc ggtggtggcc cgcccaacat gtcgatccgg ggccttggca ccgacggttt
     6601 caacaagcag ccctcggtgc gcgtctatca g
//
LOCUS       DASRYE010000329         6884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_189446,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000329 DASRYE010000000
VERSION     DASRYE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189446"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFF98_17070"
     CDS             complement(<1..266)
                     /locus_tag="VFF98_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZU65403.1"
                     /translation="MPNSRPQSRIARVIGAVNVGSFRIAAMIAGVTESGELLVLGSGH
                     RAAQGVKRGYVIDMRSATYAIRDAIERAEKMANTSVSGVWVGTAG"
     gene            complement(266..1234)
                     /locus_tag="VFF98_17075"
     CDS             complement(266..1234)
                     /locus_tag="VFF98_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZU65404.1"
                     /translation="MNRKITRKPTSARRAAQRQGAARKVEAARLRTGSVLDRAMALVP
                     LGEEGLHKLFLGAILVGAVGLMGLVAVLAGVPAMAEQHVAQIATHAGFEVRHVEVRGT
                     HHLNELRVYERALAQRNRAMTEVDLAALRADLLDLSWVSDARVSRQLPDTLVVDIVER
                     QPHAVLRQPDRLVLIDETGHLLEPVSPARAKGRLVLSGAGVEGQVQALSGLLDAAPAL
                     GPQVAEAQWVGNRRWNLLFKTGQMLALPEGDTEASAALVQFARMDGTNRLLGGKVVAF
                     DMRSPDRIYLRVPGSGAAASEAARPAAGASAAASATATATASKGAN"
     gene            complement(1259..2233)
                     /locus_tag="VFF98_17080"
     CDS             complement(1259..2233)
                     /locus_tag="VFF98_17080"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZU65405.1"
                     /translation="MSLPKLHVAVLMGGWSSERAVSLMSGTGVAEALESRGHKVTRID
                     MGRDVAQRLAEAAPDVVFNALHGAPGEDGSVQGLMDLMGLTYTHSGLATSAVAMDKEL
                     TKLVLVPHGIPMPGGRSVRSEELYAGDPLPRPYVLKPASEGSSVGVAIVTDESNCGNP
                     IRADVQGPWQHFPVLMAEPFIRGRELTTAVLAGADGPRALLVTELKPKSGFYDFDAKY
                     TDGLTEHVCPAEIPDDVAEACKAIALKAHVLLGCKGTSRADFRWDDTRGVEGLFLLEV
                     NTQPGMTPLSLVPEQARHLGMAYADLVEAIIAEALADAALRAAAPTGH"
     gene            complement(2230..3120)
                     /gene="murB"
                     /locus_tag="VFF98_17085"
     CDS             complement(2230..3120)
                     /gene="murB"
                     /locus_tag="VFF98_17085"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZU65406.1"
                     /translation="MGFVPPPVAGRLTADAPLAPLVWFKSGGAAQWLFEPADEADLCA
                     FLKALPADVPVMALGLGSNLIVRDGGVEGVVVRLGKAFAGVAADGLQLTCGGGTSGIL
                     LSSSARDAGIAGLEFLRSIPGTVGGFVRMNGGAYGREVADVLVSARVVLRSGEVADWP
                     VEKLAYAYRHSALPEGAIVVSAVFAGHAGDPVAIQAEMDRIAAAREASQPLRSKTGGS
                     TFKNPQGARAWQLVDSAGCRGLALGGAQVSEKHTNFLINTGDATSTEIEALGEEVRRR
                     VQEQSGIALEWEIKRVGKAK"
     gene            complement(3120..4559)
                     /gene="murC"
                     /locus_tag="VFF98_17090"
     CDS             complement(3120..4559)
                     /gene="murC"
                     /locus_tag="VFF98_17090"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZU65407.1"
                     /translation="MKGVGVEIGTIHFVGIGGIGMSGIAEVMANLGYKVQGSDIAEGY
                     VVEGLRARGITVFIGHSADNLGDAAVVVTSTAVKRDNPEVVAALEARIPVVRRAEMLA
                     ELMRLKNTVAVAGTHGKTTTTSMVAALLDAGGVDPTVINGGIINSYGSNARLGASDWM
                     VVEADESDGSFLRLDGTIAVVTNIDPEHLDHYGSFDKVKASFVEFIENVPFYGAAVLC
                     IDHPEVQAIIPKVRDRRVITYGFSAQADVRGESVTPIPGGNRFTAVLRARDGSFQRIE
                     GIELPMPGRHNVQNALAAVAVAKEMGVADAVIRTGFAKFGGVKRRFTKVGELDLGGGD
                     PVAIIDDYGHHPVEIRAVLAAAREGVKGRVIAVVQPHRFTRLRDHMGEFAGAFNDADI
                     VYVAPVYPAGEHPIAGVHAAALVEGIKARGHRSAHQVQGASELAATLAGQIRPGDMVV
                     CLGAGDITKWAAGLGAGIAAARAEAAGAA"
     gene            complement(4556..5722)
                     /gene="murG"
                     /locus_tag="VFF98_17095"
     CDS             complement(4556..5722)
                     /gene="murG"
                     /locus_tag="VFF98_17095"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZU65408.1"
                     /translation="MSFVQRHYVLAAGGTGGHLIPAFALASELMARGHHVALITDARG
                     AAIPGKPDGLAVHVLPAGRFQGRNPASWLKAVRAIMAGRRMALRLFDSFQPSCVVGFG
                     GYPALPALLAARAAGIATVVHEQNAVLGRVNRFFAGRVNAIATAAEQVDRLKAAHAGK
                     VHLVGNPVRTEVLALRDRPFPAFSEDGLLRILVTGGSQGARVLSEVVPDGLAMLPPAL
                     RTRLQVTQQCRPEDIDAVRARYAAHDIPAELGTYFEDMAGRLADAHLFIGRAGASTIA
                     ELTAVGRPAILVPLPIATDDHQAANTREIVAAGGARSIRQARFSAVELAKQILAMAQH
                     PETLANAAHAAWNCGYPRAAQDLADLVESFGGAPLMDVIRLGGKRRASGQEQLA"
     gene            complement(5719..6873)
                     /locus_tag="VFF98_17100"
     CDS             complement(5719..6873)
                     /locus_tag="VFF98_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZU65409.1"
                     /translation="MELAIWWREIDRLLLVLVLALMGVGTLAVAAASPASARRLSTAA
                     THLDDLHFFALQLRWQAVGLVALFGLALQKRETARRLGILFGFAMLAALLLVPVVGST
                     VNGSRRWLGMGILSLQPSEFLKPAFAILLAWVLSWKQRDPRLPVMTICCALMALVAGL
                     LMMQPDFGATVLFACVWFVEILLAGLTLRRIAMVGALGVGGVGAAYLFYANARNRIDS
                     FLSGGTAYDQVDLAHHTLVGGGWTGTGLWLGTQKLRLPEAHTDYIFSVIGEEFGLLAC
                     ALLVALYMAIVARVLVRLADREEDYFTLLAASGLVAQLGGQAFINILVNLQLFPSKGM
                     TLPLVSYGGSSTVALCAGVGFLLAITRRNPYLSRDPFARPGQGFAAGHHR"
BASE COUNT         1151 a         2561 c         2182 g          990 t
ORIGIN      
        1 cccgccgtgc ccacccacac gcccgaaacc gaggtgttgg ccatcttctc ggcccgctcg
       61 atcgcatcgc ggatggcata ggtggccgaa cgcatgtcga tcacatagcc gcgcttcacc
      121 ccttgggcgg cgcgatggcc gctgcccagc accagcagct cgccgctttc cgttacgccg
      181 gcgatcatcg cggcgatgcg gaaggagccg acattgaccg cgccgatcac ccgggcgatg
      241 cgcgattggg ggcgggaatt gggcatcagt tggccccctt gctggctgtg gcagtggcag
      301 ttgcgctggc ggcggcagaa gcgccggcag cgggccgggc ggcctcgctg gcggccgccc
      361 cgctgcccgg cacgcgcaga taaatccggt cgggcgagcg catgtcgaag gccaccacct
      421 tgccgcccag caagcggttg gtgccatcca tgcgggcaaa ctgcaccagc gcggcgctgg
      481 cctcggtgtc gccctcgggc agggccagca tctggccggt cttgaacagg aggttccagc
      541 gccggttgcc cacccattgc gcctcggcca cttgcgggcc cagcgcaggc gctgcatcca
      601 gcaggccgga aagcgcctgc acctggcctt ccaccccggc gcccgaaagc accagccggc
      661 ccttcgcccg cgccggcgag accggctcca gcaggtggcc ggtctcgtcg atcagcacca
      721 gccggtcggg ctggcgcagc accgcatggg gctggcgctc gacgatatcg accaccagcg
      781 tatcgggcag ctggcgcgag acccgcgcat cgctcaccca cgacagatcg agcagatcgg
      841 cgcgcagggc ggccagatcc acctcggtca tcgcccggtt gcgctgggcc agcgcccgct
      901 catacacgcg caattcgttg aggtgatggg tgccgcgcac ttccacatgg cgcacctcga
      961 agccggcatg ggtggcaatc tgggcgacat gctgctcggc catcgccggc acgcccgcca
     1021 gcaccgccac cagccccatc agccccacag cgcccaccag aatggcgccc aggaacagct
     1081 tgtgcagccc ttcctcgccc agcggcacca gcgccatcgc ccggtccagc acggaaccgg
     1141 tgcgcaggcg cgccgcctcc accttgcgcg ccgcgccctg ccgctgggca gcgcggcggg
     1201 cgctggtggg tttgcgggtg atcttgcggt tcatggccag ccccccggcg ccccttccct
     1261 agtggcctgt gggcgcggcc gcccgcagcg cggcatcggc cagcgcctcg gcgatgatcg
     1321 cttccaccag atcggcatag gccatgccca gatggcgcgc ctgttcgggc accaggctga
     1381 gcggggtcat gcccggctgg gtgttcactt ccagcaggaa cagcccttcc accccgcgcg
     1441 tgtcgtccca gcggaaatca gcgcggctgg tgcccttgca gcccagcagc acatgggcct
     1501 tcagcgcgat cgccttgcag gcctcggcca catcgtcggg gatctcggcc gggcacacat
     1561 gctcggtcag gccatctgtg tatttggcgt cgaaatcata gaagccgctc ttgggcttca
     1621 gctcggtcac cagcagggcg cgggggccat cggcgccggc cagcacggca gtggtcagct
     1681 ccctgccgcg gatgaaaggc tctgccatca gcaccgggaa atgctgccag gggccttgca
     1741 catcggcgcg gatggggttg ccgcaattgc tctcgtcggt cacgatcgcc acgcccacgc
     1801 tcgaaccctc gctggccggc ttcagcacat aggggcgcgg cagcggatcg ccggcgtaaa
     1861 gctcttcaga gcgcaccgaa cgcccgcccg gcatggggat gccatggggc accagcacca
     1921 gcttggtcag ctccttgtcc atcgccacgg ccgaagtcgc caggcccgaa tgggtgtagg
     1981 tgaggcccat cagatccatc agcccctgca cgctgccatc ctcgccgggg gcgccatgca
     2041 gggcattgaa caccacatcg ggcgccgctt ctgccagccg ctgggccaca tcgcggccca
     2101 tgtcgatccg cgttacctta tggccgcggc tttccagcgc ttcggccacg cccgtgccgc
     2161 tcatcaacga caccgcccgc tccgacgacc agccgcccat cagcacggcc acatgcaatt
     2221 tcggcaggct cacttcgctt ttcccacgcg cttgatttcc cattcaaggg caatgcccga
     2281 ctgttcctgc acccggcggc gcacttcctc gcccagcgcc tcgatctcgg tgctggtggc
     2341 atcgccggtg ttgatgagga aattggtgtg cttctcgctg acctgcgcac cgcccagcgc
     2401 caggccccgg cagccggcgc tgtccaccag ctgccaggcc cgcgcgccct gcgggttctt
     2461 gaaggtggag ccgcccgtct tgctgcgcag gggctggctg gcctcgcgcg cggcggcaat
     2521 gcggtccatc tcggcctgga tggcgacagg atcgccggca tggccggcaa acaccgcgct
     2581 gaccactatc gccccttccg gcagcgccga atggcgatag gcataggcca gcttttccac
     2641 cggccaatcc gccacttcgc cactgcgcag caccacccgc gcactcacca gcacatcggc
     2701 cacttcgcgg ccataggcgc cgccgttcat ccgcacgaag ccgcccaccg tgccggggat
     2761 ggagcgcagg aattccagcc ccgcaatccc ggcatcacgc gcgctggagg agagcagaat
     2821 gccgctggtg ccgccgccgc aggtcagctg caggccatcg gcggcaacgc cggcaaaggc
     2881 cttgcccagc cgcaccacca cgccctccac accgccatcg cgcacgatga ggttgctgcc
     2941 cagccccagc gccatcactg gcacatcggc cggcagggcc ttgaggaacg cgcagagatc
     3001 cgcctcgtcg gccggttcga acagccattg cgccgccccg ccagacttga accagaccag
     3061 cggcgccagc ggcgcatcgg ccgtcaaccg gccggccaca ggaggcggca caaagcccat
     3121 caggccgcgc ccgccgcttc ggcccgcgcg gccgcaatgc cggcaccaag gccggcggcc
     3181 catttggtga tatcgcccgc gcccaggcac accaccatgt cgccggggcg gatctggccg
     3241 gccagcgttg ccgccagctc gctggcgccc tgcacctgat gggcgctgcg atggccgcgc
     3301 gccttgatcc cttccaccag cgcggcggca tgcacgccgg ctatcggatg ctcgcccgcc
     3361 ggatagaccg gcgccacata gacgatatcg gcatcgttga aggcgccggc aaattcgccc
     3421 atgtgatcgc gcagccgggt gaagcggtgc ggctgcacca cggcgatgac gcggcccttc
     3481 accccttcgc gcgcggccgc cagcacggcg cggatctcta ccgggtgatg gccataatca
     3541 tcgatgatcg ccaccggatc gccgccgccc agatccagct cgcccacctt ggtgaagcgc
     3601 cgcttcacac cgccaaactt ggcaaagccg gtgcggatca ccgcatcggc cacgcccatt
     3661 tccttggcca ccgccactgc cgccagcgcg ttctgcacat tgtggcggcc gggcatcggc
     3721 agctcgatcc cctcgatccg ctggaagctg ccatcacgcg cgcgcagcac ggcggtgaag
     3781 cggttgccgc cggggatcgg cgtcacgctc tcgccgcgca catcggcctg ggcggaaaag
     3841 ccataggtga tgacgcggcg atcgcgcacc ttggggatga tcgcctgcac ttcgggatgg
     3901 tcgatgcaca gcaccgccgc gccatagaag ggcacgttct cgatgaattc gacaaagctc
     3961 gccttcacct tgtcgaacga gccgtaatgg tcgagatgtt cgggatcgat gttggtgacg
     4021 acggcaatcg tgccatccag ccgcaggaag ctgccatcgc tctcgtcggc ttccaccacc
     4081 atccagtcgc tggcgcccag ccgggcgttg gagccatagg aattgatgat gccgccgttg
     4141 atcaccgtcg ggtccactcc gccggcatcc agcagcgctg ccaccatcga ggtggtcgtc
     4201 gtcttgccat gggtgccggc caccgccacg gtgttcttca gccgcatcag ctcggccagc
     4261 atttcggccc ggcgcaccac cggaatgcgc gcttccagcg ctgccaccac ttcggggttg
     4321 tcgcgcttca ccgctgtcga ggtgacgacg accgccgcat cgcccagatt gtcggcgctg
     4381 tggccgatga acacggtaat gccgcgcgcg cgcaggcctt ccaccacata gccctcggca
     4441 atgtcggagc cctgcacctt gtagcccagg ttggccatca cctcggcaat gcccgacatg
     4501 ccaatgccgc cgatgccgac aaaatgaatg gtgccgatct cgacgcctac gcccttcatg
     4561 ccaattgctc ctgcccgctg gcgcggcgct tgccgcccag ccggatcaca tccatcagcg
     4621 gtgccccgcc aaaactctcg accagatcgg ccagatcctg cgccgcacgc ggatagccgc
     4681 agttccatgc ggcatgggcg gcattggcca gcgtttcggg gtgctgggcc atggcgagga
     4741 tctgcttggc cagctccacc gcgctgaagc gcgcctggcg gatcgaacgc gcgccgcccg
     4801 ccgccacgat ctcgcgcgtg ttggcggcct ggtggtcgtc tgtcgcgatc ggcagcggca
     4861 ccaggatcgc cgggcggcca acggcggtca gctcggcaat ggtgctggcc ccggcgcggc
     4921 cgatgaacag atgcgcatcg gccagccggc cggccatatc ctcgaaatag gtgcccagtt
     4981 cagcgggaat gtcatgcgcg gcatagcggg cgcgcacggc atcgatatct tccggacggc
     5041 actgctgggt cacctgcaga cgcgtgcgca gggccggcgg cagcatcgcc agcccatcgg
     5101 gcaccacttc cgagagcacc cttgccccct gcgatccgcc ggtcaccaga atgcgcagca
     5161 gcccatcctc gctgaaggcc gggaacgggc gatcgcgcag cgccagcact tctgtgcgca
     5221 ccgggttgcc caccagatgc accttgccgg catgggcggc cttcagcctg tccacctgct
     5281 cggccgccgt ggcgatggca ttgacgcgcc cggcaaagaa gcggttgacc cggcccagca
     5341 ccgcgttctg ctcatgcacc accgtggcaa tgccggccgc ccgcgccgcc agcagcgccg
     5401 gcagcgccgg atagccgcca aagcccacca cgcaggaagg ctggaaactg tcgaacaggc
     5461 gcagcgccat gcgccggccg gccatgatcg cgcgcaccgc cttcagccag ctggccgggt
     5521 tcctgccctg gaagcgcccg gccggcagca catgcaccgc cagcccatcg ggcttgccgg
     5581 gaatggcagc gccgcgcgca tcggtgatca gcgccacatg gtggccgcgc gccatcagct
     5641 cgctggccag cgcgaaggcg ggaatgagat gcccgccggt gcccccggcg gccagaacat
     5701 aatggcgttg cacaaaactc atcgatgatg tcccgcggcg aagccctggc ccgggcgggc
     5761 gaaggggtcg cggctcagat aggggttgcg ccgcgtgatg gcgagcagga atccgacccc
     5821 ggcgcacagc gccacggtgg atgacccgcc ataagagacg agcggcagcg tcatgccctt
     5881 ggaagggaac aattgcaggt tgacgaggat gttgatgaag gcctgcccgc ccagctgggc
     5941 caccagcccg ctggccgcca gcagggtgaa gtaatcctct tcccgatcag ccagccgcac
     6001 cagcacccgc gccacgatcg ccatatacag cgccaccagc agggcgcagg ccaacaggcc
     6061 gaattcctcg ccgatgaccg agaagatata gtccgtatgg gcctctggca ggcgcagctt
     6121 ctgggtgccc agccacaggc cggtgcctgt ccacccgccg cccaccagcg tgtgatgggc
     6181 cagatccacc tgatcgtaag ccgtgccgcc cgacaggaaa ctgtcgatcc ggttgcgcgc
     6241 gttggcatag aagagatagg ccgcgcccac gccgcccacc cccagcgccc ccaccatggc
     6301 aatgcgccgc aaggtgaggc cggccagcaa tatctccacg aaccacacgc aggcaaacag
     6361 cacggtggcg ccaaaatcgg gctgcatcat caacaggccc gccaccagcg ccatcagcgc
     6421 gcagcagatc gtcatcaccg gcaggcgcgg atcgcgctgc ttccacgaca gcacccaggc
     6481 cagcaggatg gcaaaggccg gcttcaggaa ttccgacggc tgcagcgaga ggatgcccat
     6541 gcccagccac cggcgcgagc cattgaccgt gctgcccacc accggcacca gcagcagcgc
     6601 ggccagcatg gcaaagccga agagaatgcc cagccgccgc gccgtctcgc gcttttgcag
     6661 ggccaggcca aacagcgcca ccagccccac cgcctgccag cgcagctgca gggcaaagaa
     6721 atgcagatca tccagatgcg tggccgcggt ggacaggcgg cgggcgctgg ccgggcttgc
     6781 cgctgccacc gccagcgtgc ccacccccat cagcgccagc accagcacca gcagcagccg
     6841 gtcgatctcg cgccaccaga tcgccagttc catgcgccag cggc
//
LOCUS       DASRYE010000330         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_189537,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000330 DASRYE010000000
VERSION     DASRYE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189537"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1324
                     /locus_tag="VFF98_17105"
     CDS             335..1324
                     /locus_tag="VFF98_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZU65410.1"
                     /translation="MLIHPLLGALADPTRLRIMRLLCAMELAVGELAQVLGQSQPRVS
                     RHVRILCEAALAERRKEGSWVFLKSALGLPADKGLGGAVARLLAEAEGTDAGFAEVCA
                     RDRRQLAAIRAGREAAAAAYFARHAAQWDGMRSLLGEEAPVEAALARALGTDLGALLD
                     IGTGTGRMAELFAPAAAHVTAFDKSPDMLRIARARLQHLPAESVDLVQGDFAALPFGP
                     ARFDTVLFHQVLHYAQAPEAVLAEAARVARPGARLAVVDLAAHTREELRDAHAHARLG
                     FADGQMQALFAGAGFDAAEPLAIPGAELTVKIWTGTRRPEPTPIPQTEKALFP"
     gene            1321..2268
                     /gene="metF"
                     /locus_tag="VFF98_17110"
     CDS             1321..2268
                     /gene="metF"
                     /locus_tag="VFF98_17110"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZU65411.1"
                     /translation="MIASYEALSEARKALDAPLFAGLPGDVRVSFEFFPPKSEAMMAQ
                     LWDVVETLAPLNPDFVSVTYGAGGTTRDRTHDTVARIIAEAHLPAAAHLTCVDASKDE
                     IRQVAESYWQAGVRHIVALRGDMGQPGAPFVPHPQGYANAAELVAGLKAIAPFEISVA
                     AYPEKHPDSADVAADIDNLKRKFDAGASRAITQFFFEPETFFRFRDKLAAAGIHAPIL
                     PGILPVTNFAQASKFAAVCGAAMPGWMAGLFAGLDRLPAARQLVAATVAAEFCRKLYA
                     GGVRDFHFYTLNRAELSYAICHLLGVRPQAIAPRAETLA"
     gene            2265..3326
                     /locus_tag="VFF98_17115"
     CDS             2265..3326
                     /locus_tag="VFF98_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZU65412.1"
                     /translation="MTLAPSPARAALLAQAAKRLLITDGAQGTEFQKLGLVEADFAGT
                     LGLAHEQKGNNDMLSLSRPDVVEALHRSYFEAGADLAETNTFNANRISQADYGAQHLV
                     RELNIASAQIARRVADEYTARDGRPRFVAGGVGPTNKTLSLSPDVNDPGYREVDWDTL
                     VDVYAEQVAALVEGGADFILIETVFDTLNAKAGIMAVRHVEQQLGREIPIMLSMTLTD
                     LSGRNLSGHTVEAFWYAVRHARPLTIGLNCSFGATQLRPHVKLLSEIADTLIMVYPNA
                     GLPNELGAYDELPETTAALVKEWADAGQVNILGGCCGSTPAHIGAMARAVAGLAPRHA
                     PGLPHVTRLAGLEPFTMVE"
     gene            3332..5995
                     /gene="metH"
                     /locus_tag="VFF98_17120"
     CDS             3332..5995
                     /gene="metH"
                     /locus_tag="VFF98_17120"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZU65413.1"
                     /translation="MNASSSSRPAGGASGARFVNVGERTNVTGSAAFKKLILAGDYAK
                     AVEVARSQVDNGAQIIDVNMDEGLLDAVVAMTTFLKLLAAEPDIARVPVMIDSSKWEV
                     IEAGLKCVSGKPIVNSISMKEGEDAFLHHARLCMDYGAAVVVMAFDEKGQADTEARKV
                     EICTRAYRLLTGIGFAPEDIIFDPNIFAVATGIEEHDNYGVDFIEATRAIKAACPHVH
                     ISGGLSNLSFSFRGNEPVRRAMHSVFLYHAIPAGMDMAIVNAGQLDIYDQIDPTLRAA
                     CEDVILNRPLPEGSPYETNTERLIAIAETFKGRDKAAEKEAEEWRSYEVTRRLEHALV
                     KGIDAFIVEDTEEARAAIAARGGRPIEVIEGPLMGGMNTVGDLFGSGQMFLPQVVKSA
                     RVMKKAVAHLIPFIETEKDAGARSKGRILMATVKGDVHDIGKNIVGVVLQCNGYEVID
                     LGVMVPWAKIIETAQAEEVDIIGLSGLITPSLDEMVTVAEEMNRAGLHQGKTAVPLLI
                     GGATTSKVHTALRIDPAYEGPVVHVLDASRAVGVASQLLSDTQASAFIEATASDYEAV
                     RVARAGKGGSRLVSLADARANAFAPDFALKPAAPAQPGLHRFDDWDLADLAQYIDWTP
                     FFRAWELAGVYPAILQDAVVGESARSLFADAQAMLARIIAEKWLIARGVCGFWPAVRD
                     EDDIVLADGTVLPMLRQQFAKSRGRANFCLADFIDPEGDWLGGFAVGIHGIEPHLAAF
                     KAAHDDYSDILLKALADRFAEAFAERLHQHVRTTLWAYAAGEQLTNEALIREQYRGIR
                     PAPGYPACPDHSLKPILFDLLDATENTGITLTESQAMLPTAAVSGFYFAHPESQYFGV
                     ATIGEDQLADYAERRGVDVEVARRWLRPNLD"
     gene            6055..6999
                     /locus_tag="VFF98_17125"
     CDS             6055..6999
                     /locus_tag="VFF98_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZU65414.1"
                     /translation="MRSFLAPPLAAMALLAATPPALAQNPAPFTIAETGRHFARLQEA
                     VNAIGGHTGTIRIAAGNWHECAVQQAGSIAYVAEQPGQVVLSGTICEGKAALVLRGTA
                     ARVDGLTFANLRVAEGNGAGIRLEKGNLAVSQSWFRDSEEGILTADDPHASITIDKST
                     FTHLGRCDRGLSCAHALYTGDYGSLAVTRSRFEAGSGGHYLKSRAARVTIEDNSFDDT
                     AGHTTNYMIDLCAGATGRIAGNWFVQGRDKENWSTFIAIAAESRSHPSAGLVVEGNVA
                     RFAPGLSRANAFVADWSGAPLVIRQNQLGAGISPFQRR"
BASE COUNT         1140 a         2453 c         2303 g         1136 t
ORIGIN      
        1 gcaccgcggc cacgccgctg atcgcctctt cagccggcat ttccacgctg ctgccggcat
       61 cggccgccgc ttcttcgcca tgatggccac aagcgcccag cgccagcagg gcgccaaggg
      121 caaaagcggc ggggagcaga gggtggcggc gcatgggctt ctccttgggg gcggggcatg
      181 cgagggagga ctggcgcctt tctagcgcca gcggcaggcg ggcagcaaga gggcggcagg
      241 ggccggccgc gtttggctgg ggaaagcggg cgcgcgtggc ggttgcaatc atataaagat
      301 atctttatat gcgccccagc gagaacagac acccatgttg attcatcccc tcctcggcgc
      361 gctggcagat cccacccggc tgcgcatcat gcgcctctta tgcgccatgg agctggccgt
      421 gggcgagctg gcgcaagtgc tggggcaaag ccagccgcgc gtctcgcgcc atgtgcgcat
      481 cctgtgcgag gcggcgctgg ccgaacggcg caaggaaggc agctgggtgt tcctgaaaag
      541 cgcgctgggc ctgcccgccg acaagggcct gggcggcgcg gtggcccgcc tgctggccga
      601 ggcggaaggc accgatgccg gctttgccga agtgtgcgcg cgtgatcgcc ggcagctggc
      661 cgccatccgc gccgggcgcg aggccgccgc ggccgcctat tttgcccgcc acgccgccca
      721 atgggatggc atgcgcagcc tgctgggcga ggaagccccg gtggaagcag cgctggcccg
      781 ggcgctgggc accgatctgg gcgcgctgct cgatatcggc accggcaccg gccgcatggc
      841 cgaactgttt gcgcctgccg ccgcccatgt caccgccttt gacaagagcc ccgacatgct
      901 gcgcatcgcc cgcgcccggc tgcagcatct gccggccgaa agcgtggatc tggtgcaggg
      961 cgattttgcc gccctgccct ttggcccggc gcgcttcgat acggtgctgt tccatcaggt
     1021 gctgcattat gcccaggcgc ccgaggccgt gctggccgag gccgcccgcg tggcccgccc
     1081 cggcgcgcgc ctggccgtgg tcgatctggc cgcccacacc cgcgaggaac tgcgcgatgc
     1141 ccacgcccat gcgcgcctgg gctttgccga cgggcagatg caggcgctgt ttgccggcgc
     1201 cggcttcgat gcggcagagc cgctggcgat ccccggcgcc gagctgaccg tgaagatctg
     1261 gaccggcacc cgccgccccg agcccacccc cataccacag accgaaaagg cccttttccc
     1321 atgatcgcca gttacgaggc gctgagcgaa gcgcgcaagg cgctcgatgc accgctgttt
     1381 gccggcctgc ccggcgatgt gcgcgtgtcc ttcgagtttt tcccgccgaa gagcgaggcg
     1441 atgatggccc agctgtggga tgtggtggaa acgctggccc cgctgaaccc ggatttcgtc
     1501 tcggtcacct atggcgcggg cggcaccacg cgcgatcgca cgcatgacac ggtggcgcgc
     1561 atcatcgccg aggcgcatct gccggctgcc gcccatctca cctgcgtcga tgcgtcgaag
     1621 gacgagatcc gccaggtggc cgaaagctac tggcaggcag gcgtgcgcca tatcgttgcg
     1681 ctgcgcggcg acatgggcca gcccggcgcc cccttcgtgc cgcacccgca aggctatgcc
     1741 aatgccgccg agctggtggc cggcctcaag gccatcgccc cgttcgagat ctcggtggcg
     1801 gcctatccgg aaaagcaccc tgattcggcc gatgtcgccg ccgatatcga caatctgaag
     1861 cgcaagttcg atgccggcgc cagccgcgcc atcacccagt tcttcttcga gccggaaacc
     1921 ttcttccgct tccgcgacaa gctggccgcc gccggcatcc atgcccccat actgccgggc
     1981 atcctgccgg tgacgaattt tgcccaggcc agcaagtttg ccgcggtctg cggggcggcc
     2041 atgccgggct ggatggccgg cctgttcgcc gggctggacc gcctgcccgc cgcccgccag
     2101 ctggtggccg ccaccgtggc cgccgaattc tgccgcaagc tctatgccgg cggcgtgcgc
     2161 gatttccact tctacacgct caaccgggcg gaactgtcct atgccatctg ccacctgctg
     2221 ggcgtgcgcc cgcaagcgat agccccccgc gcggagaccc tcgcatgacc cttgccccct
     2281 cccccgcccg cgccgccctg ctggcccagg cggccaaacg cctgctcatc accgatggcg
     2341 cgcagggcac cgaattccag aagctgggcc ttgtcgaggc ggatttcgcc ggcacgctgg
     2401 gccttgccca cgagcagaag ggcaacaacg acatgttgtc gctctctcgc ccggatgtgg
     2461 tggaggcgct ccaccgctcc tatttcgagg ccggcgccga tctggccgag accaacacct
     2521 tcaacgccaa ccgcatcagc caggccgatt atggcgccca gcatctggtg cgcgaactga
     2581 acatcgcctc ggcgcagatc gcccgccgcg tggccgatga atataccgcc cgcgatggcc
     2641 gcccgcgctt tgtggccggc ggtgtgggcc ccaccaacaa aacgctctcg ctcagccccg
     2701 atgtgaacga cccgggctat cgcgaggtgg attgggacac gctggtcgat gtctatgccg
     2761 agcaggtggc ggcgctggtg gaaggggggg ctgatttcat cctcatcgaa accgtgttcg
     2821 acacgctgaa cgccaaggcc ggcatcatgg ccgtgcgcca tgtggaacag cagctgggcc
     2881 gcgagatccc gatcatgctg tcgatgacgc tgaccgatct ttccggccgc aacctgtcgg
     2941 gccacacggt ggaggcgttc tggtatgccg tgcgccatgc ccggccgctg acgatcgggc
     3001 tcaactgctc cttcggcgcc acgcaattgc gcccgcatgt gaagctgctg tcggagatcg
     3061 ccgatacgct gatcatggtc taccccaatg cgggcctgcc caacgagctg ggcgcctatg
     3121 acgagctgcc ggaaaccacc gcggcgctgg tgaaggaatg ggccgatgcc gggcaggtga
     3181 acattctggg cggctgctgc ggatcaacgc cggcccatat cggcgcgatg gcccgcgcgg
     3241 tggccggcct tgcgccgcgc catgcgcccg gcctgccgca tgtgacccgc ctggcgggcc
     3301 ttgaaccttt caccatggtc gagtgagacg catgaacgct tcttcttctt cccgccccgc
     3361 aggcggcgct tcgggtgccc gcttcgtcaa tgtcggcgag cgcaccaatg tcaccggctc
     3421 ggccgccttc aagaagctga tcctcgccgg cgattatgcc aaggccgtgg aggtggcgcg
     3481 cagccaggtg gacaatggtg cgcagatcat cgacgtcaac atggacgagg gcctgcttga
     3541 tgccgtggtg gcgatgacca ccttcctcaa gctgctggcc gccgagcccg acatcgcccg
     3601 cgtgccggtg atgatcgaca gctccaagtg ggaagtgatc gaggccgggc tcaaatgcgt
     3661 ctcgggcaag ccgatcgtca attcgatcag catgaaggag ggcgaggacg ccttcctgca
     3721 tcatgcgcgc ctgtgcatgg attatggcgc ggcggtcgtc gtcatggcct tcgacgagaa
     3781 gggccaggcc gataccgagg cgcgcaaggt ggaaatctgc acccgcgcct accggctgct
     3841 gaccgggatc ggctttgccc ctgaagacat catcttcgat cccaacatct ttgccgtggc
     3901 caccggcatt gaagagcatg acaattacgg cgtggacttc atcgaggcca cccgcgcgat
     3961 caaggccgcc tgcccgcatg tgcatatctc gggcggcctg tcgaacctgt cgttctcctt
     4021 ccgcggcaac gagccggtgc gccgggccat gcattcggtg ttcctctacc atgccattcc
     4081 ggcagggatg gacatggcga tcgtcaatgc cggccagctc gacatctacg accagatcga
     4141 ccccaccttg cgcgcggctt gcgaggatgt gatcctcaac cgcccgctgc ccgaaggcag
     4201 cccctacgag accaacaccg agcggctgat cgccattgcc gaaaccttca agggccgcga
     4261 caaggccgcc gagaaggaag ccgaggaatg gcgctcctat gaggtgacgc gccggctgga
     4321 acatgcgctg gtgaagggca tcgacgcctt catcgtggag gatacggaag aagcgcgcgc
     4381 ggccatcgcg gcacgcggcg gccggccgat cgaggtgatc gaggggccgc tgatgggcgg
     4441 catgaacacg gtgggcgatc tgttcggatc gggccagatg ttcctgccgc aggtagtgaa
     4501 atcggcccgg gtgatgaaga aggcggtggc ccatctcatc cccttcatcg agaccgagaa
     4561 ggacgccggc gcccgctcca agggccgcat cctgatggcc acggtgaagg gcgatgtgca
     4621 tgatatcggc aagaacatcg ttggcgtggt gctgcagtgc aacggctatg aggtgatcga
     4681 cctgggcgtg atggtgccct gggccaagat catcgagacg gcgcaggccg aagaggtgga
     4741 tatcatcggc ctgtccggcc tcatcacccc cagcctcgac gagatggtga cggtggccga
     4801 ggaaatgaac cgcgccggcc tccaccaggg caagacggca gtgcccctcc tcatcggcgg
     4861 ggccaccacc agcaaggtgc acaccgcgct gcgcatcgat ccggcctatg aggggccggt
     4921 ggtgcatgtg ctggatgcca gccgcgcggt gggcgttgcc agccagctgc tctccgacac
     4981 ccaggccagc gccttcatcg aggccaccgc cagcgattac gaagccgtgc gcgtggcccg
     5041 cgccggcaag ggcggatcaa ggcttgtcag cctggccgac gcgcgcgcca atgcctttgc
     5101 gcccgatttc gcgctgaagc cggccgcccc cgcccagccc ggcctccacc gtttcgacga
     5161 ctgggatctg gcagacctcg cccagtatat cgactggacg cccttcttcc gcgcctggga
     5221 gctggccggg gtctatcccg ccatcctgca ggatgcggtg gtgggcgaaa gcgcgcgcag
     5281 cctgtttgcc gatgcccagg ccatgctggc ccgcatcatc gcggaaaagt ggctgatagc
     5341 gcgcggcgtc tgcggcttct ggccggccgt gcgcgacgag gacgacatcg tgctggccga
     5401 tggcacggtg ctgcccatgc tgcgccagca atttgccaaa tcgcgcggcc gggccaattt
     5461 ctgccttgcc gatttcatcg atcccgaggg cgactggctg ggcggctttg ccgtgggcat
     5521 ccatggcatc gagccgcatc tggccgcctt caaggccgcc cacgacgatt acagcgacat
     5581 cctgctgaaa gccctggccg accgctttgc cgaagccttt gccgaaaggc tgcaccagca
     5641 tgtgcgcacc accttgtggg cctatgccgc cggcgagcag ttgaccaacg aggcgctgat
     5701 ccgcgagcaa tatcgcggca tccgcccggc gcccggctat cccgcctgcc cggatcacag
     5761 cctgaagccg atcctcttcg acctgctgga tgccaccgaa aacaccggta tcacccttac
     5821 cgaaagccag gccatgctgc ccacggcggc cgtctcgggc ttctatttcg cccatccgga
     5881 aagccagtat ttcggcgttg ccaccatcgg cgaggaccag ctggccgatt atgcggaacg
     5941 gcgcggcgtg gatgtggaag tggcccggcg ctggctgcgc cccaatctcg attagggaca
     6001 cttggcccgc ggccagcctc ttcggccgcg ggcccgggcg gggtataagg cgccatgcgt
     6061 agcttccttg ccccgccgct ggccgccatg gccctgcttg ctgccacccc gccagcgctg
     6121 gcgcaaaacc ccgcgccttt caccattgcc gaaaccgggc gccactttgc ccggctgcag
     6181 gaagcggtga acgccatcgg cggccatacc ggcacgatcc gcatcgccgc cggcaactgg
     6241 catgaatgcg ccgtgcagca ggccggcagc atcgcctatg tggccgaaca gccgggccag
     6301 gtggtgcttt cgggcaccat ctgcgaaggc aaggccgcgc tggtgctgcg cggcacagcc
     6361 gcccgggtgg acggcctgac ctttgccaac ctgcgcgtgg ccgagggcaa tggcgccggc
     6421 atccggctgg aaaagggcaa ccttgccgtc agccagagct ggtttcgcga ttccgaggaa
     6481 ggcatactga ccgccgacga cccccacgcc agcatcacca tcgacaaatc caccttcacc
     6541 cacctgggcc gctgcgaccg gggcctctcc tgcgcccatg cgctctacac cggcgattat
     6601 ggcagccttg ccgtcacccg cagccggttc gaggcgggca gcggcggcca ctacctcaaa
     6661 agccgcgccg cccgcgtgac gatcgaggac aattccttcg acgacaccgc cggccacacc
     6721 accaactaca tgatcgacct gtgcgccggc gccaccgggc gcattgccgg caactggttc
     6781 gtgcaggggc gcgacaagga gaactggtcc accttcatcg ccattgccgc cgaaagccgt
     6841 agccacccgt cggccggcct ggtggtggaa ggcaatgtgg cgcgctttgc cccgggcctt
     6901 tcgcgcgcca acgcctttgt cgccgattgg tctggcgcgc cgctggtgat ccgccagaac
     6961 cagctgggcg ccggcatcag ccccttccag cggcgttgag attgcggcac cggcccgcgc
     7021 ccccacccgg cc
//
LOCUS       DASRYE010000331         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_173771,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000331 DASRYE010000000
VERSION     DASRYE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173771"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFF98_17130"
     CDS             <1..666
                     /locus_tag="VFF98_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65415.1"
                     /translation="AGSVALATPAAVVAGLLPPPDPDPPPIPVIPLDFALAADWPARM
                     ASLAQPATLTIAERERLRGAGPTLATAGGGLDPLGKGHIAELAGFAFSYGNTLAANAL
                     GTRLRLDRSTSQLAMRVDLNGAKPLAHATPMALSYDGNLSYTYANRLSLGVVARGDLG
                     TFGNFAPPADHVAGAVAKLQFHPFGTSFNAETDYLVTMGPAALATPPNLRVKLNLAVP
                     LGR"
     gene            complement(676..1590)
                     /locus_tag="VFF98_17135"
     CDS             complement(676..1590)
                     /locus_tag="VFF98_17135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU65416.1"
                     /translation="MASPPQISRFALYGEGNGAIAPEFLHIESISSRSSLYEWTISPH
                     SHSGIFQLLMLEKGGVVLASDAGRARLAPPALVAVPAGCVHAFHFDEGSEGWVLSIAL
                     DLLNDPRLGWVLEQSPLLGAEVLFRAFAGQGAGRLPEAMAGLAEGLAAGRSGELARSV
                     VAQLAVVLALADAALEQAGAGAAPAGHGGRHEQLVHRFRRLVEAHFREGWSVERYAAG
                     LGATAPTLTRACRQMIGKAPGEAVLDRLLLEAMRALTYTAAPISQIAADLGFEDPAYF
                     ARFFKRRVGMTASHFRHSRAWLTREAHT"
     gene            1758..3806
                     /locus_tag="VFF98_17140"
     CDS             1758..3806
                     /locus_tag="VFF98_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZU65417.1"
                     /translation="MTYRLGVDVGGTFTDLLLFDQTSGQFWRHKTPSTPHDSSEGILN
                     GALAICAKAAISPADIEFFLHGTTVATNAVLEGKGARVGLITTRGYRQMMQIARSFVP
                     GGLAGWIVWPKPEPLAALEDTIEIAGRMDAQGREIAATDEAEIRAALAALKAAGVEAL
                     TVSLVNAYLNGTHEALVGELAAEIMPGVPVSLSHVVLPEMQEYERTLTTVANASVRPV
                     VGNYVHNLRNRLRGAGLAGSLSLLRSDGGLMSSEKAGEQPVNLLMSGPAGGVTGAIWV
                     GRNAGIKNILTLDVGGTSTDVALIENLEARRQRTTEVGHLSVRASALDVKTVGAGGGS
                     IAYVPELTRALRVGPQSAGAVPGPVAYQKGGTLPTVTDANVVLGYLPENLLGGTFQLD
                     REGARKAVQTIADALGIGLMEAARGIIDIVNENMFGALRMISVQQGYDPRDFALMGFG
                     GAGPLHVNAVARLMGSWPAVSPVSPGVLCALGDATTRMRTETARSFSRRLADTDEEAV
                     ITLLGEMAGQTSAELLAEGIAPADITTLFELDVRYEGQAFEVPLAIDPETLLRDGLKA
                     VTARFDAEHLRLFTFNMDSAHELVNLRAVAMGPALELPAPPLPQTDGHVERAKIRDHE
                     LWADGAMVPAIIYDRARLLAGDIIPGPAVVVEMDSTTLIEAGCTGTVDHLGNILITLA
                     "
     gene            3822..5699
                     /locus_tag="VFF98_17145"
     CDS             3822..5699
                     /locus_tag="VFF98_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZU65418.1"
                     /translation="MPAQIIQANDTPFAPVSVDPVTLDIIENALRNARIEMDATLVRT
                     AMSPGIREQGDAFPLIADHQGRMIVGQFGSYIGPFLESYEGTVEEGDMIFLSDPYSVG
                     GAISHCNDWLVLLPVFKDGRLIAYTSMFGHQSDIGGKVPGSMPIDATSIFQEGVRIPP
                     VKIWKQGVYNEDLVRLVMHQSRTPDWCKADLNALIASCRVAARRVQEMAARFGDDVFI
                     AATDLLLERNYRAMQQLIATSIGEEKVSFEDYICDDGMGYGPYKIKCTMWREEGPQGS
                     RVVLDFAGTDPQSQASINFLLNENMMKMFFGIYMIMVFDPQILFNDGYYPLIDVRIPE
                     GSLLKPKFPAALSGRTHALGRLFDILGGLLGQKTPEFLNAAGFSSSPHLFYAGTDKAG
                     QWFQLFQIGFGGIPGRPLGDGPDGHSLFPGFTNVPNEFLERYFPLVIERYETVPDSGG
                     PGLHRGGNGIDMVYRFLEPGVIAIHDDRWFVPPWGVNGGLPGARARKILVKADGSEKV
                     IGNKLEEYPVEAGDRLHFITWGGGGWGDPLERAPELLLREVAQGLVSVAGARRYGVVL
                     TDGGVDAGATAALRAEMRAARGPVGTFDFGPDIETLRANCLAETGLPAPIQPRWRAAT
                     Q"
BASE COUNT          989 a         2047 c         1915 g          949 t
ORIGIN      
        1 gccggcagcg tggcgctggc gacgcctgcg gccgtggtgg cagggctgct gcccccgccc
       61 gatcccgatc cgccgccgat ccccgtcatc cccctcgatt tcgcgctggc ggccgattgg
      121 ccggcccgca tggcaagcct cgcccagccc gccacgctga ccatcgccga gcgcgaaagg
      181 ctgcgcggcg ccggccccac gctggccaca gccggcggcg ggctcgatcc gctgggcaag
      241 ggccatatcg ccgagttggc cggctttgcc ttttcctatg gcaacacgct ggccgccaat
      301 gcgctgggca cccggctgcg gctcgatcgc tcgaccagcc aactggccat gcgggtggat
      361 ctcaatggcg ccaagccgct ggcccatgcc acgcccatgg ccctctccta tgatggcaac
      421 ctgtcgtaca cctatgccaa ccggctcagc cttggcgtgg tggcgcgcgg cgatctgggc
      481 actttcggca atttcgcgcc gccggccgat catgtggcgg gcgcggtggc caagctgcag
      541 ttccaccctt tcggcaccag cttcaacgcc gagaccgact atctggtgac gatgggcccg
      601 gccgcgctgg caaccccgcc caacctgcgg gtgaagctca atctggcggt gccgctgggg
      661 cgttgaaggc cggtttcagg tatgcgcctc acgtgtcagc caggcgcggc tgtggcggaa
      721 gtggctggcc gtcatcccca cccgccgctt gaagaaacgg gcgaaatagg ccggatcctc
      781 aaagcccaga tcggcggcga tctggctgat cggcgcagcg gtgtaggtca gggcgcgcat
      841 cgcttccagc agcagccggt cgagcaccgc ctcgcccggc gccttgccga tcatctgccg
      901 gcaggcgcgg gtgagcgtgg gcgccgtggc gccaaggccg gcggcatagc gctccacgct
      961 ccagccctca cggaaatggg cctccaccag ccggcggaaa cggtgcacaa gctgctcgtg
     1021 ccggccgcca tggccagccg gcgccgcccc tgccccggcc tgttccagcg ccgcatcggc
     1081 cagcgccagc accaccgcca gctgcgccac cacgctgcgc gccagctcgc ccgagcggcc
     1141 cgccgccagc ccttcggcca gccccgccat cgcctcgggc aggcggccgg cgccctgccc
     1201 ggcaaaggcg cggaacagca cctcggcccc cagcagcggc gattgctcca gcacccagcc
     1261 cagccgcgga tcgttcagca gatcgagcgc gatcgacagc acccagcctt ccgagccctc
     1321 gtcgaaatga aaggcatgca cgcagcccgc cggcaccgcc accagcgccg gcggcgccag
     1381 ccgcgcccgg cccgcatcgc tggccagcac caccccgccc ttttccagca tcagcaactg
     1441 gaaaatgcca gaatgggaat ggggcgagat cgtccattca taaaggctgg agcgcgagga
     1501 aatcgattcg atatgcagga attccggcgc gatggcacca tttccctcgc cataaagggc
     1561 aaagcggctg atctgggggg ggctggccat gggcgccatt catgccgccg ctgcatgcaa
     1621 agtgcaagcc gccagccaaa cgacgatcct cacgcgataa ttgtgtacaa ttaatttgta
     1681 aacgttatca ttcccagact gcgccggccc atccatcagc ccgcgcgggg attcggctct
     1741 aaccattcgg ggatctcatg acctacagat tgggcgttga tgtgggcggc acgttcaccg
     1801 acctcttgct tttcgatcag accagcggcc agttctggcg ccacaagacg ccctccaccc
     1861 cgcatgacag ttccgaaggc atcctgaacg gcgcgctggc gatctgcgcg aaagcggcga
     1921 tcagcccggc cgatatcgag ttcttcctgc atggcacaac ggtggccacc aacgccgtgc
     1981 tggagggcaa gggcgcgcgg gtggggctga tcaccacgcg cggctatcgg cagatgatgc
     2041 agatcgcccg cagctttgtg cccggcgggc tggccggctg gatcgtctgg cccaagcccg
     2101 agccgctggc cgcgctggaa gacacgatcg agatcgccgg ccgcatggat gcgcaaggcc
     2161 gcgagatcgc cgcgacagac gaggccgaga tccgcgccgc ccttgccgcc ctcaaggccg
     2221 ccggggtgga agcgctcacc gtcagcctcg tcaacgccta tctgaacggt acgcatgagg
     2281 ccctggtggg cgaactggcg gccgagatca tgcccggcgt gccggtctcg ctcagccacg
     2341 tggtgctgcc cgagatgcag gaatacgaac gcacgctgac gaccgtggcc aatgccagcg
     2401 tgcggccggt ggtgggcaat tatgtgcaca atctgcgcaa ccggctgcgc ggcgcggggc
     2461 tggccggatc gctcagcctg ctgcgctccg atggcgggct gatgtcctcg gaaaaagccg
     2521 gggaacagcc cgtcaacctg ctgatgagcg ggccggccgg cggcgtgacc ggggcgatct
     2581 gggtgggccg caatgccggc atcaaaaaca tcctcacgct ggatgtgggc ggcaccagca
     2641 ccgatgtggc gctgatcgag aacctggagg cgcgccgcca gcgcaccacc gaagtgggcc
     2701 atctttcagt gcgcgcctcg gcgctggatg tgaaaacggt gggcgccggc ggcggctcca
     2761 tcgcctatgt gccggaactg acgcgggcgc tgcgggtggg cccgcaatcg gcaggcgcgg
     2821 tgccggggcc ggtcgcctat cagaagggcg gcaccctgcc cacggtgaca gacgccaatg
     2881 tggtgctggg ctatctgccg gaaaacctgc tgggcggcac cttccagctc gaccgcgagg
     2941 gcgcgcgcaa ggcggtgcag accatcgccg atgcgctggg cattggcctg atggaagcgg
     3001 cgcgcggcat catcgatatc gtcaacgaga acatgtttgg tgccctgcgg atgatctcgg
     3061 tgcagcaggg ctacgacccg cgtgactttg cgctgatggg ctttggcggc gccggccccc
     3121 tccatgtgaa tgcggtggcg cggctgatgg gcagctggcc ggctgtctcg ccggtcagcc
     3181 ccggcgtgct gtgcgcgctg ggcgatgcca ccacccgcat gcgcaccgaa accgcccgca
     3241 gcttcagccg ccgcctggcc gataccgacg aggaagcggt gattaccctg ctgggcgaga
     3301 tggccggcca gaccagcgcc gaactgctgg ccgaaggcat cgcgccggcc gacatcacca
     3361 ccctgttcga gctggatgtg cgctatgagg gccaggcctt cgaggtgccg ctggcgatcg
     3421 acccggaaac gctgctccgc gacgggctga aggcggtgac cgcccgtttc gatgccgagc
     3481 acctgcgcct gttcaccttc aacatggatt ccgcgcacga gctggtgaac ctgcgcgccg
     3541 tggccatggg cccggcgctg gaactgcccg ccccgcccct gccgcaaacc gacggccatg
     3601 tggaacgcgc caagatccgc gaccacgagc tgtgggccga tggcgcgatg gtgcccgcca
     3661 tcatctacga tcgcgccagg ctgctggccg gcgacatcat cccgggcccg gcggttgtgg
     3721 tggaaatgga ttccaccacg ctgatcgagg ccggctgcac cggcactgtc gatcacctcg
     3781 gcaacatcct catcactctt gcctgagaag gaggcacctt catgcccgca cagatcatcc
     3841 aggccaatga cacccccttc gcccccgtca gcgtcgatcc ggtcacgctc gacatcatcg
     3901 agaacgccct gcgcaacgcc cgcatcgaga tggacgccac gctggtgcgc accgccatgt
     3961 cgcccggcat ccgcgaacag ggcgatgcct tcccgctcat cgccgatcac cagggccgca
     4021 tgatcgtcgg ccagttcggc agctatatcg gccccttcct cgaaagctat gaaggcacgg
     4081 tggaggaagg cgacatgatc ttcctttccg atccctattc ggtgggcggc gccatctccc
     4141 attgcaacga ctggctggtg ctgctgccgg tgttcaagga cgggcgcctc atcgcctata
     4201 cctcgatgtt cggccaccag agcgatatcg gcggcaaggt gcccggatcc atgccgatcg
     4261 atgccacctc gatcttccag gaaggcgtgc gcatcccgcc ggtgaagatc tggaagcagg
     4321 gggtctacaa cgaggatctg gtgcgcctcg tcatgcacca gagccgcacg cccgactggt
     4381 gcaaggccga tctcaacgcg ctgatcgcca gctgccgggt ggccgcgcgc cgggtgcagg
     4441 aaatggccgc ccgctttggc gatgatgtgt tcatcgccgc caccgacctg ctgctggagc
     4501 gcaattaccg cgccatgcag cagctcatcg ccacttccat cggcgaggag aaggtgagct
     4561 tcgaggatta catctgcgac gatggcatgg gctatggccc ctacaagatc aaatgcacca
     4621 tgtggcgcga ggaaggccca cagggcagcc gggtggtgct cgattttgcc ggcaccgacc
     4681 cgcaaagcca ggcctcgatc aatttcctgc tgaacgagaa catgatgaag atgttcttcg
     4741 gcatctacat gatcatggtc ttcgatccgc agatcctgtt caacgatggc tattacccgc
     4801 tgatcgacgt gcgcatcccc gaaggatcgc tgctgaagcc caaattcccg gccgcccttt
     4861 cgggtcgcac ccatgcgctc gggcgcctgt tcgatattct gggcggattg ctggggcaaa
     4921 agaccccgga attcctcaat gcggcaggct tttcctccag cccccacctg ttctatgccg
     4981 gcaccgacaa ggccggccag tggttccagc tgttccagat cggctttggc ggcattcccg
     5041 gccggccgct gggcgatggg ccggatggcc attcgctgtt ccccggcttc accaacgtgc
     5101 ccaacgaatt cctcgaacgc tacttcccgc tggtgatcga gcgttatgaa accgtgcccg
     5161 acagcggcgg gccgggcctg catcgcggcg gcaatggcat cgacatggtc tatcgcttcc
     5221 tcgagccggg cgttatcgcc atccacgatg atcgctggtt cgtgccgccc tggggggtta
     5281 acggtggcct gccgggggcg cgggccagga agatcctggt gaaggccgat ggcagcgaga
     5341 aggtaatcgg caacaagctg gaggagtatc cggtggaagc aggcgaccgg ctgcatttca
     5401 tcacctgggg cggcggcggc tggggcgacc cgctggagcg cgcgcccgaa ctactgctgc
     5461 gcgaagtggc gcaggggctg gtcagcgtgg ctggcgcgcg gcgctatggc gtggtgttga
     5521 cagacggcgg cgtggatgcc ggggcaaccg ctgccctgcg cgccgagatg cgcgccgccc
     5581 gcggccctgt cggcactttc gattttggcc ccgatatcga aaccctgcgc gccaattgcc
     5641 tggccgaaac cggccttccc gcccccatcc agccgcgctg gcgcgcagcc acgcaatagg
     5701 ctccatttac acggccgggg ccccgccccg gcctcctcca aggacatctg ccgcatgagc
     5761 aaagccgccc ccgacgacct gctggacaac tatcgccgcg cctacaacac ccgcgtcggc
     5821 tttggcgcgc ggccggcgct gatcctgatc gatttctgcc agggctattt cgagcccgat
     5881 tgcgaccttt atggcggccc
//
LOCUS       DASRYE010000332         9089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_190562,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000332 DASRYE010000000
VERSION     DASRYE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190562"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFF98_17150"
     CDS             complement(<1..607)
                     /locus_tag="VFF98_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU65419.1"
                     /translation="MKHPTALHSLWEWQNMALMELRNLKVFVEVVRQGGFTEAARVLF
                     STQSTVSKAIRALEEELGLPLLERGSRRVAATAAGAIVYRQALRILGAREDLVSEIAE
                     LKGLQRGTLRLGLPQVGGDALFAPIFARFRQLYPGIEVELREYGSARLRELLHSGELD
                     LAGLLLPVPDEFGTQELGCEPVVALLPGGHRLAGAARLAFAD"
     gene            718..2463
                     /gene="dld"
                     /locus_tag="VFF98_17155"
     CDS             718..2463
                     /gene="dld"
                     /locus_tag="VFF98_17155"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HZU65420.1"
                     /translation="MPALPADSLIPPGPAAPAGLAQRLGAIVGGAHLFTGEKATRRFR
                     TGYRFGTGAVLAVVQPGSLVELWRCLRACVEAGVIVIAQAANTGLTGGSTPDGRYDRP
                     VVILSTRRLAGIHLIRDGAQVVCLPGATLYDLEARLKPLGREPHSVIGSSCIGASVLG
                     GICNNSGGALVRRGPAYTELALYARVDAGGRLELVNHLGIALGEEAEAMLARLERGDF
                     TPADVAPATDRSASDHNYADHVREIDAATPARFNADPARLFEASGSAGKVMLFAVRLD
                     TFPRDKATRVFYIGTNDPAELTALRRAILGSFAALPVAGEYLHRTCFDIAARYGKDTF
                     LAIRKLGTDRLPALFAAKARVDALPFLPAHTSDRLLQWLADRLPQHLPARLLAWRDRY
                     EHHLMLRMAGPDGDSGGIAEARAWLGAHFPSATGGFFECSQEEAEAAFLHRFAAAGAA
                     IRYRAVHARAVEDIVALDIALPRNCEDWVEELPPAIAAPILHKLYYGHFLCHVFHQDY
                     IVAKGVDCHVLEQSMLPLLDRRGAEYPAEHNVGHLYAAKPALSAHYEALDPCNMFNPG
                     IGHSCRDARHPGAPA"
     gene            2516..4240
                     /gene="poxB"
                     /locus_tag="VFF98_17160"
     CDS             2516..4240
                     /gene="poxB"
                     /locus_tag="VFF98_17160"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HZU65421.1"
                     /translation="MPTIADQMVETLAAAGVRRIWGIVGDSLNGFTDAIRRRGGIEWL
                     HVRHEEVAAFAAAGEAETTGELAVCAGSCGPGNLHLINGLFDAHRSRVPVVAIAAHIP
                     SAEIGSGYFQETHPEELFRECSSYCELVSNVAQMPRALEIAIRRAVAERCVSVIVIPG
                     DIALKEIPAISRPEALSLLPPRPSVVPPADAIAHLAAMLNGAERVTLLCGGGVAGAHD
                     EVVALARALKAPVVHALRGKQHIEWDNPHSVGMTGLIGFSSGYHAMGACDMLLMLGTD
                     FPYRQFYPDNAKVAQVDLRPEVIGRRTRVDLGLIGDVKETLAALLPHVAEKTQDAHLR
                     ASIAHYREARTALDKLATGKPGGRLHPQHVMRVLSEKASADAAFTADVGLPTVWSARY
                     LEMNGQRSLTGSFWHGSMANAMPQAMGIQAAHPGRQVISLSGDGGFTMLMGDMLSLKQ
                     LGLPVKVVVFNNSSLGFIELEQKSSGFVDFGTGFENPDFAAMANAVGIKGIRIEDPAD
                     LEAGIAAALAHPGPVVIDAVVERMELAMPPKVTGEMAKGFTLYMIKAVLNGRGDEVVE
                     LARANLLR"
     gene            4518..4979
                     /locus_tag="VFF98_17165"
     CDS             4518..4979
                     /locus_tag="VFF98_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZU65422.1"
                     /translation="MFSFSRLMLAGACALTIASAADAAGHATSAQSQGLLDKAVAELK
                     AAGPDKAFAEFNQPQGAYTTHELYVFVFSLDGVYEASGANPKLVGTSAIDTKDAEGKP
                     LVREMIDVAKAKGHGRVDYVWLNRGDNRVEHKRSLVQRVGNHIVGVGYYAG"
     gene            complement(5010..5903)
                     /locus_tag="VFF98_17170"
     CDS             complement(5010..5903)
                     /locus_tag="VFF98_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZU65423.1"
                     /translation="MQRHTMNDLLAFRAVAQERSFTRAAARMGVSPSALSHALRALEE
                     RLGLRLLARTTRSVAPTAAGERLLATIGPRFDEIESELAALGDLSQRPAGTIRITTSS
                     HAAQAILWPALAPLLAAYPDIAVELSVNAGFVDIVAERFDAGVRLGETIAQDMIAVRI
                     SPDMEMAAVAAPAYLRRRPPPATPQDLAAHNCINLRFPTHGSLYAWEFEKADRALNVR
                     VEGQLTVNEIALARQAALDGLGIAYLPEDHVRAELASGRLRRVLADWCPPFAGYHLYY
                     PSRRQHSQAFALLVEALRWRG"
     gene            5999..6325
                     /locus_tag="VFF98_17175"
     CDS             5999..6325
                     /locus_tag="VFF98_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZU65424.1"
                     /translation="MTTQPPTMRAPWADIAPHLTQISDEVLFGDVWQHPALSARDRSL
                     ATVASLIALYRQNELPFHMAKALENGVSRDELIGLVTHLAFYAGWPCASTALTIARRL
                     FDETPA"
     gene            6359..7381
                     /locus_tag="VFF98_17180"
     CDS             6359..7381
                     /locus_tag="VFF98_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZU65425.1"
                     /translation="MHTRELGRSGLSVSAIGLGCMGISFSYATSLSKAEGVALIRGAV
                     DRGVTFFDTAEVYGPLVNEEVVGEALAPLQDKVVIATKFGFDIDPVTGENRGISSRPE
                     HIRRAVEGSLKRLRVEAIDLLYQHRVDPAVPIEDVAGTVKELIAAGKVKHFGLSEPGV
                     GTLRRAHAVQPVAAVQNEYSLWWRAPESNGILAACDELGIGFVPYSPLGKGFLTGAMG
                     ADTRFGAGDFRNTLPRFAPEALAQNVALVDLLKRIAAEKSVTPAQIALAWLLAQRPYI
                     VPIPGTTKLARLEENVGAAALTLSAGELEVIGAAAAAIPIEGERYAPQHLAWVGREAP
                     PASQPA"
     gene            7405..8439
                     /locus_tag="VFF98_17185"
     CDS             7405..8439
                     /locus_tag="VFF98_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZU65426.1"
                     /translation="MRATIMHHAHDVRIEHVPDAAICHPTDALIRITRACICGSDLWP
                     YNDGPGAPARAMGHEAIGAVLEVGSAVQRIRPGQVVIMPFAYAEGHCVFCAEGLPTSC
                     THGGFFGVGGDAGGAQAEALRVPLADGTLYPLPMGEDDALMPALLTLSDVMGTGHHAA
                     LSARVRPGASVAVVGDGAVGLCGVIAARRLGAEQIILLGRHEDRIRLARHFGATDVVR
                     SRGAEALEEVRQLSGGHGVHSVLECVGSAQAIETALGIVRPGGAVGRVGVPHYGALPD
                     VGPMFYRNITVAGGPAPVRSYIDTLLPDILEGRIEPGLVFDSTVDLAGVPEGYRAMNE
                     RRSLKVLIRP"
     gene            complement(8458..8534)
                     /locus_tag="VFF98_17190"
     tRNA            complement(8458..8534)
                     /locus_tag="VFF98_17190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8498..8500),aa:Pro,seq:tgg)
     gene            complement(8587..>9089)
                     /locus_tag="VFF98_17195"
     CDS             complement(8587..>9089)
                     /locus_tag="VFF98_17195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU65427.1"
                     /translation="TAKVGVAAAGRLPTVKFTGFLGLGGSHPGDVVDVTSLTTVLAPQ
                     ISWNFLDFGGNLAKLRQARGGREEAEAKYREAVLAALRDAEDSLARYGAARTRLASAL
                     ASRQEAVRLEALASARFAAGTATRADALKAERDRLAADQSVAEAEAELTGAYVVVQKA
                     LGLGWQ"
BASE COUNT         1398 a         3201 c         3008 g         1482 t
ORIGIN      
        1 gatcggcaaa ggccagccgc gcggcgcccg ccagccggtg cccgccgggc agcagcgcca
       61 ccaccggctc acagcccagc tcctgcgtgc cgaattcatc gggcaccggc agcagcagcc
      121 cggccagatc cagctcgccg ctgtgcagca attcgcgcag gcgcgcgctg ccatattcgc
      181 gcagctccac ctcgatgccg ggataaagct ggcggaaccg cgcgaaaatc ggcgcgaaca
      241 gcgcatcgcc ccccacctgc ggcaggccca gccgcagcgt gccgcgctgc aggcccttca
      301 gctcggcgat ctcgctcacc agatcctcgc gcgcgcccag aatgcgcagc gcctggcgat
      361 agacgatcgc gcccgcggcc gtggccgcca cccggcgtga accgcgctcc agcagcggca
      421 ggcccagctc ctcctccagc gcgcggatcg ccttgctgac agtggactgg gtggaaaaca
      481 gcacccgcgc cgcctcggtg aagccgccct ggcgcaccac ctcgacgaag accttgagat
      541 tgcgcaattc catgagcgcc atgttctgcc attcccacag ggaatgcaag gcagtcggat
      601 gtttcatttc cagaatgggg cgccagggcc taaacccaca gccgccccag caaaggatct
      661 gcctccctca tgcctgcctc cgtttcggcc cccgtttcgg cccccgcttc ggccctcatg
      721 cccgcccttc ctgccgacag cctcatcccc cccggcccgg ccgcacctgc cgggctggca
      781 cagcgcttgg gcgccatagt cggcggcgcg catctgttca ccggcgagaa ggccacccgg
      841 cgctttcgca ccggctatcg ctttggcacc ggggcggtgc tggcggtggt gcagccgggc
      901 agcctggtgg aactgtggcg ctgtttgcgg gcctgcgtgg aagcgggcgt catcgtcatt
      961 gcccaggcgg ccaacaccgg cctcaccggc ggctccaccc ccgatggccg ctatgaccgg
     1021 ccggtggtga tcctcagcac gcggcggctg gccggcattc acctcatccg cgatggcgcg
     1081 caggtggtgt gcctgccggg cgccaccctg tatgatctgg aggcgcggct gaagcccttg
     1141 ggccgcgagc cgcattcggt gatcggctcc tcctgcatcg gcgcttcggt gctggggggc
     1201 atctgcaaca attcgggcgg cgcgctggtg cgccggggcc cggcctatac cgagctggcg
     1261 ctgtatgccc gggtggatgc cgggggccgg ctggagctgg tgaaccatct gggcattgcc
     1321 cttggcgagg aggccgaggc gatgctggcg cggctggagc gcggggattt cacccccgcc
     1381 gatgtggcgc ccgccaccga ccgctccgcc tccgaccaca attacgccga ccatgtgcgc
     1441 gagatcgatg ctgccacccc ggcccgcttc aacgccgatc cggcgcggct gttcgaggca
     1501 tcgggcagtg ccggcaaggt gatgctgttc gccgtccggc tcgacacttt cccccgggac
     1561 aaggccaccc gcgtcttcta catcggcacc aatgatccgg ccgagctgac tgccctgcgc
     1621 cgcgccattc tcggcagctt tgccgctttg ccggtggcgg gcgaatatct ccaccgcacc
     1681 tgtttcgaca tcgccgcgcg ctatggcaag gacacgttcc tggccatccg caagctgggc
     1741 acagaccggc tgccggcgct gtttgccgcc aaggcgcggg tggatgcgct gcccttcctg
     1801 cccgcccaca ccagcgaccg gctgctgcaa tggctggccg atcgcttgcc ccagcacctg
     1861 cccgcgcgcc tgctggcctg gcgcgatcgc tatgagcacc acctgatgct gcgcatggcc
     1921 ggccccgatg gtgacagcgg cggcattgcc gaggcgcgcg cctggctggg ggcgcatttc
     1981 ccctcggcca ccggcggctt cttcgaatgc tcgcaagaag aggccgaggc cgccttcctc
     2041 caccgctttg ccgcggccgg cgctgccatc cgctatcgcg ccgtgcatgc ccgcgcagtg
     2101 gaggatatcg tcgcgctcga catcgccctg ccgcgcaatt gcgaggattg ggtggaggaa
     2161 ctgccccccg ccatcgccgc gcccatcctg cacaagctct attatggcca tttcctgtgc
     2221 catgtgttcc accaggatta catcgttgcc aagggcgtgg attgccatgt gctggaacag
     2281 agcatgctgc ccctgctcga ccggcgcggg gcggaatatc cggccgagca caatgtcggc
     2341 cacctctatg ccgccaagcc cgcgctcagc gcccattacg aagcgctgga cccgtgcaac
     2401 atgttcaacc ccggcatcgg ccacagctgc cgcgatgcgc gccaccccgg cgccccggcc
     2461 tgaacctctc cctttcttcc tccccctccc cctccatccc cccaggagtt ttcccatgcc
     2521 caccattgcc gaccagatgg tcgaaaccct tgccgctgcc ggcgtgcgcc ggatctgggg
     2581 catcgtcggc gacagcctca acggtttcac cgatgccatc cgccggcgcg gcggcatcga
     2641 atggctgcat gtgcgccatg aggaagtggc cgcctttgcc gcagccggcg aggccgagac
     2701 cacaggcgag ctggcggtgt gcgccggctc ttgcgggccg ggcaacctgc acctcatcaa
     2761 cggcctgttc gatgcccatc gcagccgcgt gccggtggtg gcgatcgccg cgcacatccc
     2821 ctcggccgag atcggctcgg gctatttcca ggaaacccac ccggaggaac tgttccgcga
     2881 atgctcgtcc tattgcgagc tggtgtcgaa tgtggcgcag atgccccgcg cgctggaaat
     2941 cgccatccgc cgcgcggtgg ccgaacgctg cgtgtcggtg atcgtcatcc cgggcgatat
     3001 cgcgctcaag gaaatccccg cgatcagccg gccagaggcg ctctcgctgc tgccgccgcg
     3061 gcccagcgtg gtgccgccgg ccgatgccat tgcccatctg gccgccatgc tgaacggggc
     3121 cgagcgagtg acgctgctgt gcggcggcgg cgttgccggc gcgcatgacg aggtggtggc
     3181 gctggccagg gcgctgaagg cgccggtggt gcatgcccta cgcggcaagc agcatatcga
     3241 atgggacaac ccccacagcg tgggcatgac cgggctgatc ggcttttcct ccggctatca
     3301 cgcgatgggc gcctgcgaca tgctgctgat gctgggcacc gattttccct atcgccagtt
     3361 ctatcccgac aatgccaagg tggcgcaggt ggatctgcgg cccgaagtga tcggccggcg
     3421 cacgcgagtg gatctggggc tgatcggcga tgtgaaggaa acgctggccg cgctgctgcc
     3481 gcatgtggcc gaaaaaacgc aggatgccca tctgcgcgcc tccatcgccc attaccgcga
     3541 ggcgcgcacc gcgctcgaca agctggccac cggcaagccc ggcgggcgcc tgcaccccca
     3601 gcatgtgatg cgcgtgctga gcgagaaggc cagcgccgat gccgccttca ccgccgatgt
     3661 gggcctgccc acggtgtgga gcgcgcgcta tctggaaatg aacggccagc gctcgctcac
     3721 cggctcgttc tggcatggtt ccatggccaa tgccatgccg caggcgatgg gcatccaggc
     3781 cgcccatccc ggccggcagg tgatctcgct gtcgggcgat ggcggcttca ccatgctgat
     3841 gggcgacatg ctgagcctga agcagctggg cctgccggtg aaggtggtgg tgttcaacaa
     3901 cagctcgctg ggcttcatcg agctggagca gaagagttcc ggctttgtcg attttggcac
     3961 cggctttgaa aaccccgact ttgccgccat ggccaatgcc gtgggcatca agggcatccg
     4021 catagaagat ccggccgatc tggaagcggg cattgccgca gcgctcgccc atccggggcc
     4081 agtggtgatc gatgcggtag tggaacgcat ggaactggcc atgcccccca aggtgaccgg
     4141 cgagatggcc aagggcttca cgctctacat gatcaaggcc gtgctgaacg gccggggcga
     4201 cgaggtggtg gaactggccc gcgccaacct gttgcgctga acggaggcgg ggggcgggcc
     4261 cgcccttcgc ccgcccccgg caattgcgca tttgccctta ttgctcatcg cgcccgatgc
     4321 tgacattccc ggcaagcgcc agggcctttc cggcaaaggc ttatttcagc aacattgatc
     4381 tgcttctaag acagcggagc cgctttcggc tatctgcact aaatcaatga gttgttccgg
     4441 cacgcaatac caaagtccga aggcggacat gttctggctg cgctaggttg ggctcgtcga
     4501 taaaggagat gcagtcgatg ttttctttct cccgtctgat gctggccggc gcctgtgccc
     4561 tcaccatcgc ttctgccgcc gatgccgccg gccacgccac ttcggcccag agccaggggc
     4621 tgctcgacaa ggcggtggcc gaactgaagg cggccgggcc agacaaggcc tttgccgaat
     4681 tcaaccagcc ccagggcgcc tacaccacgc atgaactcta tgtcttcgtg ttcagcctcg
     4741 atggcgtgta cgaagcctcg ggcgccaatc ccaagctggt gggcaccagc gcgatcgaca
     4801 ccaaggatgc cgaaggcaag ccgctggtgc gcgagatgat cgacgtggcc aaggccaagg
     4861 gccatggccg ggtggattat gtgtggctga accgcggcga caaccgggtg gagcacaagc
     4921 gctcgctggt gcagcgggtg ggcaaccata tcgtcggcgt gggctattac gccggctgat
     4981 ctccggcgcg gataggggcc cgccggccgc tatccgcgcc agcgcagcgc ttccaccagc
     5041 agggcaaagg cctgcgaatg ctggcgccgg ctgggataat agaggtgata gccggcaaag
     5101 ggcgggcacc agtctgccag cacgcggcgc aggcggccgc tggccagttc ggcgcgcaca
     5161 tgatcctcgg gcagataggc gatgcccagc ccatcaagcg cggcttggcg ggcaagggca
     5221 atctcgttca ccgtcagctg cccttccacc cgcacattca gcgcccggtc cgccttctcg
     5281 aactcccagg catacaggct gccatgggtg ggaaagcgca ggttgatgca attgtgcgcg
     5341 gccagatcct gcggcgtggc cggcggcggg cggcggcgca gataggccgg cgctgccacc
     5401 gcggccattt ccatatccgg gctgatgcgg acagcgatca tatcctgcgc gatggtttcg
     5461 cccagcctta cgccggcatc gaaacgttcc gccacgatat cgacaaagcc ggcattcacg
     5521 ctcagctcca ccgctatatc cggataggcc gccagcaggg gcgccagcgc cggccacagg
     5581 atggcctgcg cggcatgcga gctggtggtg atgcggatgg tgccggccgg ccgctggctg
     5641 agatcaccca gcgcggccag ctcgctctcg atctcgtcga agcgcgggcc gatggtggcc
     5701 agcagccgct cgcccgccgc tgtgggggcc acgctgcggg tggtgcgcgc cagcaggcgc
     5761 aggcccagcc tttcctccag cgcgcgcagc gcatggctga gcgccgaggg tgacaccccc
     5821 atgcgcgccg ccgcgcgagt gaagctgcgc tcctgcgcca ccgcgcggaa ggccagcagg
     5881 tcgttcatcg tgtggcgctg cattgctgaa tcctgttcac aggcccttgc ggattgttct
     5941 gtctaatcgc cctggccgcc agcgcctaga tgtatctggc ccccccaagg agatcagcat
     6001 gaccacccag ccccccacca tgcgcgcgcc ctgggccgat atcgcgcccc atctcacaca
     6061 gatcagcgac gaggtgctgt tcggcgatgt ctggcagcat ccggccctgt ccgcgcgcga
     6121 tcgcagcctg gcaacggtgg ccagcctcat tgccctctat cgccagaacg agctgccctt
     6181 ccacatggcc aaggcactgg aaaacggcgt cagccgcgac gagctgatcg gccttgtcac
     6241 tcatctggcc ttctatgccg gctggccctg cgccagcacc gcgctcacca tcgcgcggcg
     6301 cctgttcgac gaaaccccgg cctgaaccgc ccgcttttcc gcacaaagga tacctctcat
     6361 gcacacccgt gaactgggcc gcagcggcct ttccgtttca gccatcggcc ttggctgcat
     6421 gggcatcagc ttcagctatg ccaccagcct ttccaaagcg gagggcgtgg cgcttatccg
     6481 cggcgcggtc gatcgcggcg tcaccttctt cgacaccgcc gaggtctatg gcccgctggt
     6541 caatgaggaa gtggtgggcg aggcgctggc gcccctgcag gacaaggtgg tgatcgccac
     6601 caaattcggc ttcgacatcg accctgtcac cggcgagaac cgcggcattt ccagccggcc
     6661 ggaacatatc cgccgggcgg tggaaggctc gctgaagcgg ctgcgggtgg aggcgattga
     6721 cctcctctac cagcaccggg tggatccggc cgtgcccatc gaggatgttg ccggcacggt
     6781 gaaggagctg atcgcggccg gcaaggtgaa gcatttcggc ctgtcggagc cgggcgtggg
     6841 cacgctgcgc cgggcccatg cggtgcagcc ggtggccgct gtgcagaatg aatattcgct
     6901 gtggtggcgc gcgccggaaa gcaacggcat cctcgctgcc tgcgacgaac tgggcatcgg
     6961 ctttgtgccc tacagcccgc tgggcaaggg cttcctcacc ggcgccatgg gggccgacac
     7021 gcgctttggc gctggcgatt tccgcaacac cctgccccgc ttcgcccccg aggcgctggc
     7081 gcagaatgtg gcgctggttg atctgctgaa gcgcatcgcg gcggaaaaga gcgtgacgcc
     7141 ggcgcagatc gcgcttgcct ggctgctggc gcagcggccc tatatcgtgc cgatccccgg
     7201 caccaccaag ctggcgcggc tggaggagaa tgtgggcgct gccgccctca ccctcagcgc
     7261 cggcgagctg gaggtgatcg gcgcggccgc cgccgccatt cccatcgagg gcgagcgcta
     7321 tgccccgcag cacctcgcct gggtgggccg cgaagccccg cccgccagcc agcccgcctg
     7381 aaaccctcac caaggagaag ccccatgcgc gccaccatca tgcaccacgc ccatgatgtg
     7441 cggatcgagc acgtgcccga tgccgccatc tgccacccca ccgatgcgct gatccgcatc
     7501 acccgcgcct gcatctgcgg cagcgacctg tggccctata acgacggccc cggcgcccct
     7561 gcccgcgcca tgggccacga ggcgatcggc gcggtgctgg aagtgggcag cgcggtgcag
     7621 cgcatccggc ccgggcaggt ggtcatcatg ccctttgcct atgccgaggg gcattgcgtg
     7681 ttctgcgcag aaggcctgcc cacttcctgc acgcatggcg gcttctttgg cgtgggcggc
     7741 gatgccggcg gcgcacaggc cgaggcgctg cgcgtgccgc tggccgatgg cacgctctat
     7801 ccgctgccga tgggcgagga cgatgcgctg atgcccgcgc ttctcaccct gtcggatgtg
     7861 atgggcaccg ggcatcatgc ggcgctttcg gcgcgggtgc ggccgggcgc cagcgtggct
     7921 gtggtgggcg atggcgcggt gggcctgtgc ggggtgatcg cggccaggcg gctgggggca
     7981 gagcagatca tcctccttgg ccgccacgag gatcgcatcc gcctcgcccg ccactttggc
     8041 gccaccgatg tggtgcgcag ccgcggtgca gaagcgctgg aagaggtgcg ccagctgagc
     8101 ggcggccatg gcgtgcacag cgtgctggaa tgcgtcggct ctgcccaggc aatcgagacg
     8161 gcgctgggca tcgtgcgccc cggcggcgcg gtgggccggg tgggcgtgcc ccattatggc
     8221 gcgctgcccg atgtggggcc gatgttctat cgcaacatca ccgtggccgg cgggccggcg
     8281 ccggtgcgca gctatatcga cacgctgctg cccgatatcc tcgaagggcg catcgagccc
     8341 ggcctggtgt tcgacagcac ggtggatctg gccggcgtgc ccgaaggcta tcgcgcgatg
     8401 aacgagcgcc gctcgctgaa agtgctgatc cgcccctgag gctgatatgg gcgggaatgg
     8461 tcggggagac aggattcgaa cctgcgaccc tctgctccca aagcagatgc gctaccaggc
     8521 tgcgctactc cccgactgat ctgcccttag gtgcgcccgg ccccccgcgc aagccctttg
     8581 cgcccattac tgccagccca gccccagcgc cttttgcacc accacatagg cgccggtcag
     8641 ctccgcctcg gcctcggcca cggactgatc ggcggccagc cggtcgcgct cggccttcag
     8701 ggcatcggcg cgggtggcgg tgccggcggc aaagcgcgcg ctggccaggg cctccagtcg
     8761 caccgcttcc tggcggctgg caagggcgct ggccagccgc gtgcgcgccg cgccatagcg
     8821 ggccaaactg tcttccgcat cgcgcagggc cgccagcact gcctcgcggt atttcgcctc
     8881 ggcttcctcg cggcccccgc gcgcctgccg cagcttggcg agattgccgc cgaaatcgag
     8941 gaagttccac gagatctgcg gggccagcac cgtcgtcagc gaggtcacgt caaccacatc
     9001 gcccgggtgc gatccgccaa ggcccagaaa gccggtgaac ttcaccgtgg gcaggcggcc
     9061 ggcggcggcc acgcccacct tggcggtgg
//
LOCUS       DASRYE010000333        16575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_174858,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000333 DASRYE010000000
VERSION     DASRYE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174858"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFF98_17200"
     CDS             complement(<1..261)
                     /locus_tag="VFF98_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65428.1"
                     /translation="MATCSIAQFAAMHGASKQAATKWKTRGVLVFSGNEVDIDASDRR
                     MRDAGLGNYRAGSTALPPVDVSVNQVDRRRQPVDAPRAPLLAD"
     gene            complement(909..1586)
                     /locus_tag="VFF98_17205"
     CDS             complement(909..1586)
                     /locus_tag="VFF98_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65429.1"
                     /translation="MLAINMRADLTGMQRALTALGGTQVPFASALALTRLAQGVAVQE
                     QRAVDQTFDSPTPFTVNSFVVRAARKTNLVSVVAIKPIAASYLRPYIEGGNRSLISRK
                     GVKQGMIVPMGAPVDQYGNLPRNALQRLKRNKDIYIGALASANGQRISGVWQWAPLAK
                     GAKLVGKNGQPPKARPGMKLLVRFEDTTQAPKHLPFYERARAYVKANAAREFDLALRK
                     ALATARR"
     gene            complement(1594..1917)
                     /locus_tag="VFF98_17210"
     CDS             complement(1594..1917)
                     /locus_tag="VFF98_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65430.1"
                     /translation="MRAVEKALRTVERLAGADASMVRWGARPWASPTFSGYLVEFDLQ
                     LQTAPGQVRPWDMGQGMRAFATMLVQLPERFADHYLVDSEIVSGEQVGDGPVLLRGKL
                     LLLAV"
     gene            complement(1917..2072)
                     /locus_tag="VFF98_17215"
     CDS             complement(1917..2072)
                     /locus_tag="VFF98_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65431.1"
                     /translation="MISLRKLRKVIDEHAAAASVPVSPAWLRQVERELSAARQHGQRP
                     AVNARGA"
     gene            complement(2069..2305)
                     /locus_tag="VFF98_17220"
     CDS             complement(2069..2305)
                     /locus_tag="VFF98_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65432.1"
                     /translation="MAAAPRASSARHRVAAPAMPALATLIDREIDALDALAAEVRGGV
                     RGPRHFDELEARASGIGQRLRAAFRGAGTGKAAE"
     gene            2656..3051
                     /locus_tag="VFF98_17225"
     CDS             2656..3051
                     /locus_tag="VFF98_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU65433.1"
                     /translation="MNRDDGATLNNGGSKLTPFGEIIREIRKSKGILLIDLARITEVT
                     PGFLSLVETGKKPIPDRLISTIVTQLELSKATEKKLLDAADLSARDYRIHMEAGADIL
                     DRRIAHAMQTGLAKMSPEKKSRILKLLEE"
     gene            3087..3605
                     /locus_tag="VFF98_17230"
     CDS             3087..3605
                     /locus_tag="VFF98_17230"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HZU65434.1"
                     /translation="MTSKDIEHVVASFRQYLGIAKGKPINTLNLIERVLPDLLSDYTF
                     CVLPDEDMPGMDGLTATGGDLTIYLSETTYVALCSGSLNAIITAAHELGHLILHSQQS
                     PAFARRTHDDERVDPEWQADRFAEMWLMPRDGVERCRSARHVAAKYGVTDEMAEKRFL
                     EVKHIEIQGELF"
     gene            complement(4484..5167)
                     /locus_tag="VFF98_17235"
     CDS             complement(4484..5167)
                     /locus_tag="VFF98_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65435.1"
                     /translation="MGKAQRLSAVAAQLAGQHRTSAKQFAQRHPAPAARERALRRQVA
                     QIAEDYGHKRQGTPATHAHAAAEAARPGTLLRLFRSGALSADEFGWAMEIGAEHARVG
                     ADVAVKGASLETRVDRSRQGDEHFFESLGRVRRAQAYSAWRRAIMVDLPARTGRARVA
                     AGGQGAAALAMIVDEVSIREAGRRWRMSERRARKLLLDALALWPDYLAEARDGIDERD
                     LARVCARLG"
     gene            complement(5191..5505)
                     /locus_tag="VFF98_17240"
     CDS             complement(5191..5505)
                     /locus_tag="VFF98_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65436.1"
                     /translation="MVCRRALVLDFVRGYIGRWGISPSLGEIAAALGTNRTRVQAAIR
                     ALAADGLLLRGKGPRALALPDQRSAALSILRQLGWAVDEPAGSVTHSSPAVPPVLDYK
                     GR"
     gene            6116..6514
                     /locus_tag="VFF98_17245"
     CDS             6116..6514
                     /locus_tag="VFF98_17245"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZU65437.1"
                     /translation="MHTPEHIAARRGRPMGTAKPTTKEQISIRLDPEVIAHFKTTGPG
                     WQARINKVLQAVIRRPNCQLVKLSGSVGGTRATSPTCESLWHLLLQTSCTCARASLSA
                     SNSRSNATTSGRSRCASAVRLNRCAAIAAS"
     gene            complement(7042..7677)
                     /locus_tag="VFF98_17250"
     CDS             complement(7042..7677)
                     /locus_tag="VFF98_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65438.1"
                     /translation="MGKEIAVRVVAQRDPDHFHTITVDVETAWVSCSCGVNYPPVFRV
                     FDGKLLLCDRLMCSHIDAVLVAGERAMVHPEDLEAANVASALVEGKIVMPGGWRGSWR
                     RNMRWRGLSKGGLARPGPAGAKTDRRVKKGAGPMVCFTGASSVPRSELIEQAVQAGWE
                     AIDRPSKHIDVLVTADPLLITKKVQVARESGVPIVSYDEWASVMEDGLLPE"
     gene            complement(8087..8518)
                     /locus_tag="VFF98_17255"
     CDS             complement(8087..8518)
                     /locus_tag="VFF98_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65439.1"
                     /translation="MGDGFRIEADGDLFLGDGLGGARRAPAPSCNLFGHVHRRRGKPV
                     DPALTRSKFVPTISAQLGISFDNLGAHRTFFAAVGDLTLFDRIVVRLHQKNVEDGDDE
                     QHRAVNEPRKKAATFAPGDQPNEQTKQRYDDQKTEHAILPV"
     gene            complement(8639..9247)
                     /locus_tag="VFF98_17260"
     CDS             complement(8639..9247)
                     /locus_tag="VFF98_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65440.1"
                     /translation="MKLRLVLMGAMTLALTGCEKFGASNSDAAGAGSGASDIAADAAS
                     PDDPPVPEHNYTDKIGDVYYYQESLSSNDTNNGQAAAHLQGFKYLGKKDTGEVMIVGV
                     NSRGFPLMAAICAVPCRVVKIGNGETYAFDRQSIIGAAIEDAINGQLIKTTLYEPEKL
                     PPIHYSQSLPLDKPTPEARYTPYSHNPGTQPDADEQDGANGQ"
     gene            complement(9313..9669)
                     /locus_tag="VFF98_17265"
     CDS             complement(9313..9669)
                     /locus_tag="VFF98_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZU65441.1"
                     /translation="MSKTAERLYQAMVDKGVDQSALAVAVGATQGAISKILKGHTVNS
                     RLLPRIAIALGVQLDWLLGKDAGSEPREEGAPAREAITAQDRELLAMLNDLPPAYRAA
                     LLQLTRAVAEIASGAG"
     gene            complement(9864..10205)
                     /locus_tag="VFF98_17270"
     CDS             complement(9864..10205)
                     /locus_tag="VFF98_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZU65442.1"
                     /translation="MIIGKRIKARIELLGISQAELARRVKLRQSTINGLVNGEQRSST
                     KLPEIARALQTTPAYLSGETDDPDSDSPDVLISAEDREVIDLLKTLPKAERGAFVTLL
                     RSLSARPASRA"
     gene            10291..10545
                     /locus_tag="VFF98_17275"
     CDS             10291..10545
                     /locus_tag="VFF98_17275"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HZU65443.1"
                     /translation="MERKRKTDQAFADAIIAVGSQVAYAKLVGRSQAAVSERLNGGKP
                     MWAEDVLAVETATGISRHALRPDLYPMEDAPAPAREGGQA"
     gene            complement(10686..10982)
                     /locus_tag="VFF98_17280"
     CDS             complement(10686..10982)
                     /locus_tag="VFF98_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65444.1"
                     /translation="MATPPKIAGIPWYQNADYPRILQIMEDAHLLPAKHKIWQEKAEK
                     AEALHKAQGVTIYRAHIDPEQFVAWCAQTGRNIDAKARIDWANLYAMRKFRGEN"
     gene            complement(11249..11503)
                     /locus_tag="VFF98_17285"
     CDS             complement(11249..11503)
                     /locus_tag="VFF98_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65445.1"
                     /translation="MSDLTLRVPLALAMGAIGIALDNTRVLARAGLISAAEVEAIAHN
                     LVSRFDSIENDEEGAKIRAFLDAQFSSKLAEIRQLALQHP"
     gene            11599..12087
                     /locus_tag="VFF98_17290"
     CDS             11599..12087
                     /locus_tag="VFF98_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65446.1"
                     /translation="MTKHRPPLTIDAALARIAGQLPGGWPEMAALLGRRESIVRAWGD
                     PDRRELIPLGCAIQLDLAHQAAGGKGAPLHEVYTLQLQLEHVTRFADGQALTHVLSSV
                     IKEAAEAESAIALSTLPDAGPRQARETLREIDEAIAALGQARTMVNHMATGGPTPADL
                     PP"
     gene            12207..12533
                     /locus_tag="VFF98_17295"
     CDS             12207..12533
                     /locus_tag="VFF98_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65447.1"
                     /translation="MTDVAISTDAPDYKHRVIKGFPRLAPFVAQMPNGPVRFKDAPDL
                     FISVFTAEGEEPCIGIMLYEPDCPDCPVGHGAIYLLPLEHVQTVIDALNRSVAEVAAR
                     AAKGAH"
     gene            12533..12676
                     /locus_tag="VFF98_17300"
     CDS             12533..12676
                     /locus_tag="VFF98_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65448.1"
                     /translation="MAPAALPAARPLAAGPARQRLRIAAVGLALAWAAILIPLAVGAL
                     RHG"
     gene            12669..12926
                     /locus_tag="VFF98_17305"
     CDS             12669..12926
                     /locus_tag="VFF98_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HZU65449.1"
                     /translation="MAKADSTGAQAADERLRLLIERIERLEEEKKGIGDDIKDVYLEA
                     KSAGYDPKAMRAVIRIRKMKPDDRRDLEAMLDVYKTALGID"
     gene            13190..13465
                     /locus_tag="VFF98_17310"
     CDS             13190..13465
                     /locus_tag="VFF98_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65450.1"
                     /translation="MSSHASGAENACPFDPAAETHYAFSEITEFELRTLVDALIGVSQ
                     LAETPPDELGPTVEPVYLSALFRALANLGHRLLEERVLRVPAAKGRR"
     gene            13465..13638
                     /locus_tag="VFF98_17315"
     CDS             13465..13638
                     /locus_tag="VFF98_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65451.1"
                     /translation="MRRHWPPQPRRSAVFRLPFGLAVGRWVPGITLAVFAALHLLDRV
                     IAGLVQCHIAGCS"
     gene            13751..13951
                     /locus_tag="VFF98_17320"
     CDS             13751..13951
                     /locus_tag="VFF98_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65452.1"
                     /translation="MPGWLSTLSRLLLTPPVIAAVLTIWVLINVAITLWACWCLRPRK
                     HLSRHQPPRAPSISREGTHDAH"
     gene            13941..14600
                     /locus_tag="VFF98_17325"
     CDS             13941..14600
                     /locus_tag="VFF98_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65453.1"
                     /translation="MLTDALKIDPLRGEALKANANALATNLRQQFIDATLARAPDFTP
                     TEAAMIEGMALSILANSVAASVINYIGPENRVGALNAFIETLHGGLLRARPNILRLVR
                     ESEAAVDQCIGQVEQLEALLQAVGARTAALTRGAHAAKARPAPLPSESLGRFPPADPD
                     ARRRLGMRPLDDAAHDGLCHIVSDGEQFAIARWDLGRKEWIFPLTQLTIAGLIPTEYV
                     P"
     gene            14597..14905
                     /locus_tag="VFF98_17330"
     CDS             14597..14905
                     /locus_tag="VFF98_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZU65454.1"
                     /translation="MSGAEPREEAILDTLRTLFEEAARTALPEDFAGAAIDSFGFDSI
                     DRVDLIMNVEDTFRITITDAEAERFFDGPTAFGQPRCVHDLAALVATHIDRAAGARPA
                     "
     gene            14902..15213
                     /locus_tag="VFF98_17335"
     CDS             14902..15213
                     /locus_tag="VFF98_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZU65455.1"
                     /translation="MRDPGRLTARQRQVLDLTADGLTSQGIADQLGISARTVDIHRAN
                     LLRVLNARSIAHAVAIVLRAEHAAALSPPASPEGRTAPAPATSRAGAPAPDAAAASPA
                     P"
     gene            15231..15587
                     /locus_tag="VFF98_17340"
     CDS             15231..15587
                     /locus_tag="VFF98_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65456.1"
                     /translation="MPALPAMPAQPATPPAIVQILPAGPAPRAGQLRPLALRTGHDLL
                     TAAQHFLRLAAQGPAAPTMPFSGDVVGTLAQALHTALLITLAHTADARETILLSPLCD
                     RLEEFLERLGAQVNHG"
     gene            15715..16074
                     /locus_tag="VFF98_17345"
     CDS             15715..16074
                     /locus_tag="VFF98_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65457.1"
                     /translation="MAGALPILQHALGLDEHGRGEAYRSHFVVGPGCEDYDICLAATA
                     AGLMRHRTCDWIVDGHIFHVTDDGRAYVAEHSPPPPKRTASQLRYQRYLDADCSLTFG
                     EWLRRQSKQARAAKVRP"
     gene            16328..>16575
                     /locus_tag="VFF98_17350"
     CDS             16328..>16575
                     /locus_tag="VFF98_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65458.1"
                     /translation="MTSLLDLMGQPQAPRCPRRPMWRAGVPVPGSDSGYVPHQDGDLR
                     WLSTGWDGRRWRGAWYRWDARRAEWRPLDPANRLCINHL"
BASE COUNT         3167 a         5610 c         4763 g         3035 t
ORIGIN      
        1 gtccgccagc agcggcgcgc ggggggcgtc aaccggttga cggcggcggt caacctggtt
       61 gaccgaaacg tcaaccggag gcagcgcggt tgacccggcc cgatagttgc cgaggccggc
      121 gtcgcgcatc cggcgatcgg aagcgtcgat gtcgacttca ttgccggaaa agaccagaac
      181 tccgcgcgtt ttccacttgg tggccgcctg tttcgaggcg ccgtgcatgg cggcaaattg
      241 ggcgatcgag caggttgcca tgggtaaacc gtcaaccgtc aaccaggggt caaccgggtt
      301 ttggggtcat agctggggag gcaacgcggc gtgcaatgtc cctcggcgct tggaggcccc
      361 ggaaggaccc acgagccccc ctcccccccc tccacgggtc gagccagccc ggccagatac
      421 gaggagatcg atagcctgct ttgcgtcctc agcggtaagc cccacacgaa aagccgtctc
      481 gaccaaccgt tcggcatgat ccgccaacgc ggctgcacga tcatcgatga ccacatagtc
      541 gggcttaccg tggcgccgca gccagcgttc gatttgccta ccgcgaccat ccctttcggc
      601 atcggtgcgc caatcctcat gccatgggcc gacaacccca tgggtaagca ggcgctcccg
      661 ggcatcctca cgtcggcgcc atgtgctgct gagcaccagt cgcgcgccgg ttgcagctat
      721 gatctcagcc aatgccgcaa tcgcagcagg ctggaattgc catatggtgt gccattcggg
      781 cgaccgattg taggttgagc catcccgccg gacccttgcc gttcgcagtg tcgcgatccg
      841 cctactaaca ttgagcacac cgtcgatatc aagaaagacg attggatgcc cttcgttacc
      901 gccgcccatc acctccgcgc cgtcgccaac gccttgcgca gcgccaggtc gaactcccgg
      961 gcggcattcg ccttcacata ggctcgcgcc cgctcataga acggcagatg cttgggcgcc
     1021 tgcgttgtat cctcgaaccg aaccagcagc ttcatgccgg gccgagcctt gggtggctgc
     1081 ccatttttgc cgacaagctt ggcgcccttt gccaagggcg cccattgcca cacgccggat
     1141 attcgctgtc cgttagcgct ggcgagcgcg ccaatataga tatccttgtt gcgcttcagg
     1201 cgttgcaaag cgttccgcgg taggttgcca tattggtcaa ccggagcccc cataggcacg
     1261 atcattccct gcttcacacc cttgcggctg attagggagc gattgcctcc ctcgatatag
     1321 ggccgcagat agcttgcggc gatcggcttg atcgccacca ccgacaccag attggttttg
     1381 cgcgccgcgc gcaccacaaa gctgttgacg gtaaatggcg tcgggctgtc gaatgtctgg
     1441 tcgaccgctc gttgctcctg caccgccacg ccctgcgcca gcctcgtcag cgccagcgcc
     1501 gaggcaaacg gcacctgcgt gccgcccagc gccgtcaacg cccgctgcat gccggtaagg
     1561 tccgcccgca tgttgatcgc cagcatcgcg ccctcacacc gccagcagca gcagcttgcc
     1621 gcgcagcagc accgggccgt ccccgacctg ctcaccgctg acgatctcgc tatcgaccag
     1681 ataatggtcg gcaaagcgct cgggcaactg caccagcatc gtggcaaagg cgcgcatccc
     1741 ctgccccata tcccagggcc gcacctgccc cggcgctgtc tgcagctgca gatcgaactc
     1801 cacaagatac ccgctgaagg ttggcgaggc ccaaggccgt gcgccccagc gcaccatcga
     1861 ggcatccgcg ccggccaggc gctccaccgt ccgcagcgcc ttctcaaccg cgcgcatcag
     1921 gcgccccgcg cgttaaccgc cggccgctgg ccgtgctggc gcgcggcgct cagctcgcgc
     1981 tccacctgcc gcagccaggc cggcgacacc ggcacgcttg ccgccgccgc atgctcgtcg
     2041 atcaccttgc gcaatttcct gaggctgatc attccgctgc cttccccgtt ccagcgccgc
     2101 gaaaggccgc gcgcagccgc tggccaatgc cgctcgcccg cgcctcgagc tcatcgaaat
     2161 gccgcggccc ccgcacaccg ccgcgcacct cggccgccag cgcatccagc gcatcgatct
     2221 cgcgatcgat cagcgttgcc agcgccggca tggcaggcgc cgccacacgg tgccgggcgg
     2281 aagaggcgcg gggtgcagca gccatggcga gccaacagaa aagcccgccg gcgagcgatc
     2341 accggcgggc tgcagagaga gggaggcgcg agcaagacgc aattcgtgcg gggttgaaat
     2401 tgcccgccat gctgcgtttt ggggaggctc tattttgtta cgctgtcgcc ttcagagccg
     2461 ccgtccaatg acgcttgctg atcgtcgaaa gcgagccttc gttcatccgg taccctaggc
     2521 ctgaagcttc cggtaaacgc agcgcggttc aatgcacaat tttaccactt tccggtaaat
     2581 tccgccttgc gcagttttac ctgaattggt aaatgggtgt aatccattgg tgggtggatt
     2641 ggtcggagaa ctcagatgaa ccgggacgat ggtgcaactc tgaataacgg gggcagtaaa
     2701 ttgacaccgt tcggagagat cattcgggag atacgcaaat cgaaaggcat tttgctgatc
     2761 gatctggcgc gcattaccga agtgacaccc ggtttcctga gcttggttga aaccggtaaa
     2821 aagcccattc ctgatcgcct catctcgact atcgtgacac agctggagct gagcaaggca
     2881 accgagaaga aattgctcga cgcggcggat ctttcggctc gagattaccg gatccacatg
     2941 gaggcgggag ctgatattct tgatcgccga attgctcatg cgatgcaaac cgggttggcg
     3001 aagatgtcgc cggagaagaa gtcgcgcatc ctcaagcttc ttgaggagta agcaggatgg
     3061 tcggggggtt cagggcagta aatccgatga ccagcaagga catcgaacac gtcgtcgcaa
     3121 gctttcggca atacctcggc attgccaagg gaaagcccat caacacgctt aaccttattg
     3181 agcgggttct gcctgacctc ctttctgact ataccttctg cgttctcccc gacgaggaca
     3241 tgccagggat ggacggtctt actgcaacgg gcggtgattt gacgatctac ttgtcggaga
     3301 caacttacgt tgccctttgc agcggatcgc taaatgcaat aatcactgca gctcatgagc
     3361 tcggccatct cattttacac tcccagcagt ctccagcgtt cgccaggcga acgcatgacg
     3421 acgagcgagt cgacccggaa tggcaggctg atcgcttcgc agaaatgtgg cttatgcctc
     3481 gtgacggagt tgagcgctgc aggagcgcaa ggcatgtcgc ggcaaaatat ggggttaccg
     3541 acgagatggc tgaaaagcgc tttttggaag tgaaacacat cgaaattcaa ggggaactgt
     3601 tctgatgccc ccgacacaaa aaaggaggca cggtatgccc cgtaccccct aggaaaagcc
     3661 tgaaacagaa agcgcccctg tgaaggtgag ctatcctgat ccgagcctct gactaagcgc
     3721 actcataagc gcagccggtg aaggaagcaa taacgtcggg cgcaaattat gaggtgagcc
     3781 atgccgccac cgccgctggg taagcggtgg gtgtgtcgtc cgtggaaaac ccttccggat
     3841 ggaactcgcg tttacgctag ccagtatggc aagcgcgcgt tctgctggtt ggaagacgac
     3901 gagtaagcac ccacccggcc gtcactgcaa ccacggtggc ggccaccccc gtggtcgata
     3961 tctactgctg gcatggcttc atcatttgac attcaaatcc aatttagatc ggagaaatat
     4021 atggcggtcg tctacattga agcccgtccc aaagggcgcc ctgaaggcac acccatcact
     4081 gattacgtgg ttgaagatca cgcggatcat gtattgagta cacatcacgt gcaagcagat
     4141 gcaatcgctt gggcgagggc gcgcggttat acaccgtacg ttgctcgcgt ccgccacctc
     4201 aacaataagc gcattcccga tcactggcgg gcagcctgaa gaatatcgcg gcggataatt
     4261 cctccgccgc gataatcaca agaatggtgg aaatccgcta tttataggat atcggctggg
     4321 agatcgctcc tcccaccgat attcatcacc atttgcatct caaaagagcc attctggatc
     4381 gtccttaccg tgggcatttg gtcgctgatt ttgcggacgg tgaacgaagg acagcttggg
     4441 ccaatctccc gcccagagga gacaaaccgg tctgtccccc aactcacccc aaccgcgcgc
     4501 acacccgcgc cagatcccgc tcatcgatgc cgtcgcgcgc ctcggccaga taatcgggcc
     4561 acagcgccag cgcatccagc agcagcttgc gcgcgcgccg ctcgctcatc cgccagcgcc
     4621 gccccgcctc cctgatagac acctcgtcga caatcatcgc cagcgcggcc gccccctgcc
     4681 cgccggcagc cacccttgct ctgccagtgc gcgccggtag atccaccatg atcgcccggc
     4741 gccaggcgct ataggcctgc gcccgccgca cccgccccag gctctcgaag aaatgctcat
     4801 cgccctgccg gctgcggtcc acccgcgtct ccaggctggc gcccttcacc gccacatcgg
     4861 cgcccacccg cgcatgctcg gcgccgatct ccatcgccca gccaaattca tccgcgctca
     4921 gcgcgcctga tcggaacagc cgcagcaacg tgcccggccg cgccgcctct gccgccgcat
     4981 gggcatgagt ggccggcgtg ccctgccgct tgtggccata atcctcggca atctgcgcca
     5041 cctgccggcg cagcgcccgc tcgcgcgcgg ccggcgcggg gtggcgctgg gcaaattgct
     5101 tggcgctggt gcggtgctgc ccggccagct gcgcggccac tgccgaaagt ctctgcgcct
     5161 tgcccatctg ctgctccgct gcggtgaaag tcagcggccc ttgtaatcca gcacaggcgg
     5221 cacggcgggg ctcgaatgtg tcacgctgcc ggccggctcg tccaccgccc agcccagttg
     5281 gcgcaagatg gaaagggcgg cgctgcgctg atccggcagc gccagcgcgc gcggcccctt
     5341 gccgcgcagc agcagcccat cggccgctag cgcgcggatc gccgcctgca cccgggtgcg
     5401 attggtgccc agcgccgcgg caatctcgcc caggctgggc gaaatgcccc agcggccaat
     5461 atagccccgc acaaaatcga gaacgagcgc gcgccggcaa accatcgtgg gggaaagccg
     5521 cgccccctcg cccgcttgct gtcctgctgc catcgcgccc atggcggaac aatagcagca
     5581 caatgcctaa tttttaagca cctatttcca gccgcatctc ttgcgcccat agcacttcca
     5641 acccgccatt catgccccgc gctgtgtaac aagccgcagt ggtaagcatg gtattaacgc
     5701 ggatatagtg cggggcggat ctccttctca gtcaaaaaaa tccggaaatt tgccaactgc
     5761 cttggattat ttcttgcaca gcgcattgaa cagatgttca atgcgaatcg tcaggtcatc
     5821 aatccgataa ccatcgttaa cggtgcccat caagatgaac acagacgctc cccacaaagc
     5881 ccggtgcaga gccgacgagg tcaatgcatt cctcgcaagg ctggatgcct ttctcggcta
     5941 tgatggtccg caatccgaag cgcacatccc cacgagaatt tcaagaacaa ctggcggttg
     6001 ctacaagatc aagcggtaac aggctgccct atcagctagt ctgagcctga tctctagcgc
     6061 catgcaaatg gcgcaggtgg aggcctttat cgctggccgc aaagctgaag ctgtgatgca
     6121 cacgcctgag catattgcgg cacggcgcgg gcgcccgatg ggcaccgcca agcccaccac
     6181 caaggaacag atctctatcc gcctcgatcc ggaagtgatc gcccatttca agaccactgg
     6241 ccctggctgg caagcccgca tcaataaggt gctgcaagca gtgatcaggc gccccaactg
     6301 tcagcttgtg aagctttccg gttcagtggg cggtactaga gctacatccc ccacctgtga
     6361 atcgctttgg cacctgctcc tccaaacttc ctgcacctgc gccagagctt ctctgagcgc
     6421 gagcaactcc cgctcgaacg cgaccacatc cggccgatcc agatgcgcca gcgccgtccg
     6481 attgaaccgc tgcgcggcca tagcagcctc gtgaatggtg gtgtaagagg cggtcacctc
     6541 ctgacaccca tcattggcag ccaagcgcta aggcaagcca gaaccatcac caccaacaag
     6601 agcgcggcct ccaggcgtat ccagccatcc cccggacgtg gccccttcat caccagcttg
     6661 ctactccacc gtgatgcgag caagtgccgc ggtgatgctg gctgaaactc caggagccat
     6721 ccctacattg cgcggtggcg gctgcaggac gatggctcat tcgcattgga ctatgcactg
     6781 agttgccatc cacattcgta taatgccgcc cacctgctgg gccatagcta tgatatgtcg
     6841 aatgccgggc ataatgatgt gaaatacggg catcactgac tgacgaggtc agagcaatgc
     6901 caatcaaggc aacggccgtg aataagcgct tcatggtcaa atctccttgc ccgatgggtc
     6961 tttgtctatg gggcttatgg gtggctacag ccttttcaag agacagaatc caaacttaga
     7021 cactggccgc attttacagc ctcattccgg cagaagacca tcctccatca cagatgccca
     7081 ttcgtcatag gaaacgatgg gaacgccgct ttcccgcgcg acctgtacct tcttggtaat
     7141 gagcagagga tcggccgtaa ccagcacatc aatatgcttg ctcggccggt ctatggcttc
     7201 ccaaccagcc tgcaccgcct gctcgatcaa ttcgctcctg ggcacactcg atgcgccagt
     7261 aaagcacacc atcggcccag cgcctttttt gacccgtcgg tcggtcttgg ccccagcagg
     7321 tcccggccgc gccaaacctc ctttcgaaag gccgcgccag cgcatgtttc tgcgccaact
     7381 cccgcgccac ccccctggca tgacgatttt cccctcaacc aacgcagacg ccacattggc
     7441 cgcctcgagg tcttccgggt gaaccatcgc ccgctcacca gccacaagca ccgcatcaat
     7501 gtggctgcac atcaggcggt cacacagaag cagcttgcca tcgaacaccc tgaacacggg
     7561 cggataattc acgccgcagc tgcacgaaac ccacgccgtc tcaacatcaa ccgtgatcgt
     7621 gtggaaatga tcaggatccc tttgcgcaac gaccctgact gctatctctt tgcccatttg
     7681 cggccgccta ggtgactatg tataattaca tagtcaccat ataaccgctt cctgcaacag
     7741 atttttatcc agcaccgcgg caatctcgcc caagctaggc gaaatgctcc agcggccaat
     7801 atggccccgc acaatatcga gaacgagcgc gcgccggcaa gccatcgtag gggaaagctg
     7861 cccccctcgc ccacttcctg tcctgctgcc atcgcgccca tagcggaaca acaaccgcac
     7921 actgcctaat ttttaagcaa atcttatagc ccaaacggca tttcgtcgcc cgttggcctg
     7981 cgactgcatg aagtgcagct taaacccgcc tatgaatgga aggcaattcg ctacttgatt
     8041 gagattagga ctagactccg acaaatgaac cgctagccct ccaagctcac acggggagaa
     8101 tcgcatgttc ggttttttgg tcatcgtagc gttgcttggt ttgctcgttg ggttgatcgc
     8161 ccggagcaaa ggtcgcagct tttttccttg gttcatttac ggcgcgatgt tgttcatcgt
     8221 cgccatcttc cacgttcttt tgatgaagcc gaacaacgat gcgatcgaag agcgtcagat
     8281 cgccgacggc ggcaaaaaat gtccgatgtg cgccgagatt gtcaaagctg atgccaagct
     8341 gtgccgatat tgtgggcacg aatttgctgc gagttaatgc tggatcgact ggcttaccgc
     8401 gccgccggtg cacatgcccg aacagattgc aggacggcgc gggcgcccgg cgggcaccgc
     8461 caagcccatc accaaggaac agatctccat ccgcctcgat ccggaagcca tcgcccattt
     8521 caaggccact ggccctagaa ctggtaagcc cacatcaatg agacgctgaa agcagcgatc
     8581 gggcgctagg aaatgccacg catggctatc tcaaaagcaa tgcccatggc gcacctctct
     8641 attggccatt tgccccatcc tgctcatcag catcgggttg ggttcccgga ttatgcgaat
     8701 aaggcgtgta acgcgcttct ggcgtgggct tatcgagtgg gagcgactgc gaatagtgaa
     8761 tgggcggcag cttttccggc tcgtagagcg tcgtcttgat caattgccca ttgatcgcgt
     8821 cctcgatcgc cgcgccaata atagactggc gatcaaatgc ataggtttcg ccattcccta
     8881 tcttgaccac acggcaggga accgcgcaga tcgcagccat caaaggaaat cctcgcgaat
     8941 tcacgccaac gatcataacc tctccggtgt cttttttgcc aagatattta aatccctgca
     9001 aatgagctgc ggcttggcca ttgttggtat cgttagacga aagggattcc tgataataat
     9061 acacgtcacc gattttgtcg gtgtaattgt gctccggcac gggcggatca tctggcgatg
     9121 cagcatcagc agctatatca cttgcgcccg aacccgctcc ggcagcgtca ctgttgctcg
     9181 cgccgaattt ttcgcagcca gtgagcgcca atgtcatggc gcccatcaga acaagccgca
     9241 atttcatccc atcgccccct aggtgaagtc ccgtaattcc aatcaggtgg ggacgattcc
     9301 cgcaacaggt ttttaccccg cgccgctggc aatctcggcc acggcgcggg ttagctgcag
     9361 cagggcggcg cgataggccg gtggcagatc gttcagcatg gccagcaatt cgcggtcctg
     9421 ggcggtgatg gcctcgcgcg ccggggcgcc ctcctcgcgc ggttcgctgc cggcatcctt
     9481 gcccagcagc caatccagct gcacgcccag cgcgatggca atgcgcggca gcaggcgcga
     9541 attaaccgtg tggcccttca ggatcttgct gatcgcgccc tgcgttgcgc ccaccgccac
     9601 cgccagcgcc gattgatcga cgcctttgtc gaccatcgct tggtaaaggc gctcggcggt
     9661 tttggacata gcagatgcat agggcggaac atcaggcagg attatcggat attcggaata
     9721 tgatggtatg agatagggaa taaatacagg ggaaggacag cagcagcacg gctgcaatcc
     9781 cattcggaaa atcgtttacc aaagcatttt ccgatacttt gccgaacgct gaaatcgata
     9841 ggcaacatcc atcgcgacac tcatcaggcg cgggaagcag gccttgcaga cagagagcgc
     9901 aataaagtta caaatgcccc gcgctcagcc tttggcaaag tcttcagcag gtcgataacc
     9961 tcccgatctt cggcagagat aagaacatct ggcgaatcgg aatctggatc atcggtttca
    10021 ccgctcagat aagctggggt ggtctgcagc gctcgcgcta tttccggcag cttggtggag
    10081 gatctttgct cgccgttaac caacccattt atcgtgcttt gtcttagctt tacgcgacgc
    10141 gccaattcgg cctgagaaat ccccagaagc tcgatacgtg ccttgatgcg ttttccgata
    10201 atcacggccg cactctatcg ggaatccgat agtggcgtgc gaagcatttt ccgtcttcca
    10261 tacctatcag tattccgata cgtaggagcc atggaacgca aacggaaaac cgaccaggcc
    10321 tttgcagatg ccatcatcgc tgttggctct caggtcgcat atgcgaagct ggtgggccga
    10381 tcgcaggctg ccgtctcaga gcgcctgaac ggtggcaaac ctatgtgggc tgaagatgtt
    10441 ctggccgtcg aaaccgccac gggcatttcg cggcatgccc tccggccgga tctctatccg
    10501 atggaggatg ctcccgcgcc ggcgcgggaa ggtgggcagg catgatggcc tcctttcccc
    10561 tccagcctat ggccgccgcc gcgcgccgtc tgctgcgatc ggccagccgc cttgcagctc
    10621 gccagccggc gattggccgc ctgcctgccc ctgctcataa gccgcaggcc agccgcctgc
    10681 gactttcagt tctcgccacg aaatttgcgc atggcataga ggtttgccca atcgatacgt
    10741 gccttggcat cgatgttacg acctgtctgg gcgcaccagg cgacgaactg ctccggatcg
    10801 atgtgagccc gatagatggt cacgccctgc gctttatgga gggcttccgc cttctcggcc
    10861 ttttcctgcc agatcttatg cttggcgggg agcaggtgtg catcctccat gatctggaga
    10921 atgcggggat aatccgcgtt ttgataccag gggatgcccg cgatcttcgg gggtgttgcc
    10981 attcagaatc actccatgcg gttgatgcag gcatgcatgg tagtggcccc cggggcgctg
    11041 gcaacggcgc tccggggcac atggctggag gcgccctgtg acctcgtcat caatcgggag
    11101 catcgcccca tgggctggaa gccaaaaccc aagcggcagg ccatcaacac gtggcgggaa
    11161 cggcgcgcaa ggcagcgttc ccgccgcccg gccaaaccgc tcattacaac cgattggctc
    11221 aatatggttc aagccgagct gttgcagatc atgggtgctg cagagcaagc tgccggatct
    11281 cggccagctt ggaggaaaat tgcgcgtcaa ggaaagcgcg gattttcgcg ccttcttcgt
    11341 cattttcgat ggagtcgaac cggctgacga gattgtgcgc aatggcctca acctccgcag
    11401 cgctgatcag gccggcccgg gccagaaccc tggtattgtc cagcgcgatg ccaatcgctc
    11461 ccatggcaag cgccaggggc acgcgaagcg taagatcaga cattcagaat cactccatgc
    11521 ggtcgatgca ggtatgcatg gtagtggccc ccggggcgct ggcaacggcg ctccggggcg
    11581 cagggaaggg ccccgcgcat gaccaagcat cgcccgcccc tcaccatcga tgcggcgctg
    11641 gcgcgcatcg ctggccagct gcccggcggc tggccggaaa tggccgccct gctgggccgg
    11701 cgcgaaagca tcgtgcgcgc ctggggcgat cctgatcggc gcgagctgat cccgctgggt
    11761 tgcgcaattc agctcgatct ggcccatcag gccgcgggcg gcaagggcgc ccccctgcac
    11821 gaggtctaca cgctgcagct gcagctggag catgtcacgc gctttgccga tgggcaggcg
    11881 ctcacccatg tgctctccag cgtcatcaag gaagcggcag aggcggaaag cgccattgcc
    11941 ctttccaccc tgcccgatgc cggcccccgc caggcgcgcg aaaccctgcg cgagatcgac
    12001 gaggccatcg ccgccctcgg ccaggcccgc accatggtga accacatggc caccggcggc
    12061 cccaccccgg ccgatctgcc cccgtaatcg gcgcaagggc cccgcccggc caatcctccg
    12121 cccgcatccc gccggcgcca tcctgatccc ccgccctgat cgcccatcac gctttcggga
    12181 gcggctttct gctgcccgag gttccgatga ctgatgtcgc tatctctacc gatgcgcccg
    12241 attacaaaca cagggtaatc aaaggctttc cccgcttggc gcctttcgtc gcgcaaatgc
    12301 ccaatggacc ggtgcgcttt aaagatgcgc cagatttatt catcagcgtt ttcaccgccg
    12361 agggggaaga accctgcatc gggatcatgc tctacgagcc cgattgcccg gattgcccgg
    12421 ttggccatgg cgcgatttat cttctgcccc tcgaacatgt gcagaccgtc attgatgcgc
    12481 ttaaccgctc cgtagcagag gtcgctgcgc gcgcggccaa gggcgcgcac tgatggcgcc
    12541 cgccgccttg cccgccgccc ggccgctggc cgccggcccc gcccgccagc gcctgcggat
    12601 tgccgcggtc ggcctggctc tcgcctgggc cgccatcctc atcccccttg ccgtgggagc
    12661 gctgcgccat ggctaaggca gacagcaccg gcgcgcaggc cgccgatgaa cgcctgcgcc
    12721 tcctcatcga acgcatcgag cggctggagg aggaaaagaa gggcatcggc gacgacatca
    12781 aggatgtcta tctggaagcg aaatcggccg gctacgatcc caaggcgatg cgcgccgtga
    12841 tccgcatccg caagatgaag cccgacgacc ggcgcgatct cgaagccatg ctcgatgtct
    12901 acaaaaccgc gctgggcatc gactgatgcg cctggccttg acatccggcg cacctgcgcg
    12961 catttcggca gatgttcctg cgcaaacggc aggaatgcgg actgcaaacc gccaggaatt
    13021 atgccgacaa ggcgcgctca cggatgattc cgggcgcgtt ttcctatggt cgagtgtggt
    13081 cggagggctt tgccctgccg gcttccgcat ggttccccgg ttttgcagcc ggccacgctc
    13141 ggccaccaga ctgcaaactg atggtcgggt tcatcaagga accttcacca tgagcagcca
    13201 tgcttctggc gccgagaacg cttgcccgtt cgatccggcc gccgaaaccc actacgcctt
    13261 ttccgaaatc accgagttcg aactgcgcac gctggtcgat gcgctaatcg gcgtgtcgca
    13321 actggccgaa accccgcccg atgaactcgg ccccacggtc gaaccggtgt atctttcagc
    13381 gctgttccgc gcgctggcca atctgggcca ccgcctgctg gaagaacgcg tgctgcgcgt
    13441 gccagccgcg aaagggcgac gctgatgcgc cggcactggc ccccgcagcc ccgccgcagc
    13501 gcggtgttcc gcctgccctt cggcctggct gtcgggcgct gggtgccggg tatcacgctg
    13561 gcggtcttcg ccgccctcca cctgctcgat cgcgtcatcg ccgggctggt gcaatgccat
    13621 atcgcgggct gctcctgatg tgcccggcgc ccctgcatgc gcagcgcctg cgcgatctgc
    13681 tcaccggcgc gctggtgctg gccgccgcca tggcgctggg cgccgcgctg gcctgggcgg
    13741 gggtgtgatc atgcccggct ggctcagcac cctttcccgc ctgctgctca ccccgccggt
    13801 gatcgccgcc gtcctcacca tctgggtgct gatcaacgtc gcgatcaccc tgtgggcctg
    13861 ctggtgcctg cgcccccgca agcacctgtc taggcaccag cccccgcgcg cgccgtccat
    13921 ctcccgcgaa ggaacgcatg atgctcactg atgccctcaa gattgatccc ctcaggggcg
    13981 aggctctcaa ggccaacgcc aacgcattgg ccaccaatct ccgccagcaa ttcatcgatg
    14041 ccacgctcgc ccgcgcgccc gatttcaccc cgacagaggc cgccatgatc gaaggcatgg
    14101 ccctttccat actggccaac agcgtggccg cctcggtcat caactacatc gggcccgaaa
    14161 accgggtggg cgcgctcaac gccttcatcg aaaccctcca tggcggcctg ctgcgcgccc
    14221 gccccaacat cctgcgcctg gtgcgcgaaa gcgaggcggc ggttgatcag tgcatcggcc
    14281 aggtggaaca gctggaagcc ctgctgcaag ccgtgggcgc ccgcaccgcc gccctgaccc
    14341 gtggcgccca tgctgccaaa gccaggccag cacccctgcc atcggaaagc cttggccgct
    14401 tcccgccggc cgaccctgat gcgcgccgcc ggctgggcat gcgcccgctg gatgatgccg
    14461 cccatgatgg cctgtgccac atcgtctcgg atggcgagca attcgccatc gcccgctggg
    14521 atctgggccg caaggaatgg atcttcccgc tcacccagct gacgatcgcc ggcctcatcc
    14581 ccacggaata tgtgccatga gcggcgcaga gcctcgcgag gaggcgatcc tcgacacgct
    14641 gcgcaccctt ttcgaggaag cggcccgcac agccctgccg gaagatttcg ccggcgccgc
    14701 gatcgacagc ttcggcttcg acagtatcga ccgtgtcgac ctcatcatga acgtcgagga
    14761 caccttccgc atcaccatca ccgatgccga agcggagcgc tttttcgatg ggcccaccgc
    14821 cttcggccag ccgcgctgcg tgcacgatct ggccgcgctg gtggccacgc atatcgatag
    14881 ggcagcaggg gcccggccgg catgagagat cctggccgcc tcaccgcccg ccagcgccag
    14941 gtcctcgatc tgaccgcaga tggcctcacc tcgcaaggca tcgccgacca gctcggcatt
    15001 tccgcgcgca cggtggatat ccaccgcgcc aacctgctca gggtgctcaa tgcccgctcg
    15061 atagcccatg ccgtggccat cgtcctgcgc gcagagcacg ccgccgccct ttcgcccccg
    15121 gccagcccgg aaggtcgcac cgccccggca cctgcaacca gcagggccgg ggcccccgcg
    15181 cccgatgccg ccgccgccag ccccgccccc tgatcgatcg aggaagcgcc atgccagccc
    15241 tgcccgccat gcccgctcag cccgccaccc cgcctgcgat cgtgcagatc ctgccagccg
    15301 ggcctgcccc gcgcgccggc cagctgcgcc cgctggcgct gcgcaccggc catgatctgc
    15361 tgacggccgc ccagcacttt ctgcgccttg ccgcccaagg cccagccgcc cccaccatgc
    15421 ccttcagcgg cgatgtggtc ggcacgctgg cgcaagccct gcacaccgcg ctgctgatca
    15481 ccctcgccca caccgcagac gcccgcgaaa cgatcctgct ctcccccctg tgcgaccgcc
    15541 tggaagagtt tctggagcgg ctgggcgcgc aggtgaacca tggctgacaa tgtcattcgg
    15601 cctggcgcag ggattgctcc gctaagcccg cagatctcgg gggcacacct ctgcgctggt
    15661 tccagtcagc ggaccaccga gggcgcgccg gccaatgcca agaggctacc agagttggcc
    15721 ggcgcgcttc ccatcctcca gcatgcgctg ggcctggatg aacacggccg cggcgaggcc
    15781 tatcgcagcc acttcgttgt cggcccgggc tgcgaggatt acgacatctg cctcgctgct
    15841 acagccgccg gcctgatgcg ccaccgcacc tgcgactgga tcgtggatgg gcatatcttc
    15901 cacgtcaccg atgacggccg cgcctatgtg gccgaacaca gcccgccacc gcccaagcgc
    15961 accgcttccc agctgcgcta tcagcgctat ctcgatgccg actgcagcct caccttcggc
    16021 gaatggctgc gccgccagag caagcaggcc agggccgcaa aggtgcgccc atgaccgccc
    16081 cggcgcctag gacgccatcc cccagcgcaa catcgatgca aaggtgaacc atggctgtca
    16141 cccgcgttcg gcctgcagaa ggcattcgct cgttaagaaa aatcgactcg cacatgaatg
    16201 gccgtggaaa tgcgaccgac ggccccatgc gtgagcgcgc gccggccccc gttcagaccc
    16261 tcccaggtcg ggccggcgcg cttttgcctg caagcccccg ggcccacgcg caaaatcggg
    16321 cgccagcatg acctcgctgc tcgatctgat gggccagccc caggcgccgc gctgcccccg
    16381 ccggccgatg tggcgcgccg gcgtgccggt gcccggcagt gatagcggct atgtgccgca
    16441 tcaggatggc gatctgcgct ggctttccac cggatgggat ggccggcgct ggcgcggcgc
    16501 ctggtatcgc tgggacgccc gccgcgctga atggcgcccg ctcgatcccg ccaaccgcct
    16561 ctgcatcaac cacct
//
LOCUS       DASRYE010000334         7485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_50680,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000334 DASRYE010000000
VERSION     DASRYE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50680"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..141)
                     /locus_tag="VFF98_17355"
     tRNA            complement(67..141)
                     /locus_tag="VFF98_17355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Gln,seq:ttg)
     gene            complement(265..1119)
                     /gene="yghU"
                     /locus_tag="VFF98_17360"
     CDS             complement(265..1119)
                     /gene="yghU"
                     /locus_tag="VFF98_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HZU65459.1"
                     /translation="MSQEYTPPKVWEWAKPEGDGVFSLNRPIAGATHDEALPRGQHPI
                     QLYSLATPNGQKVGILLEELLAAGHAGAEYDAWFINIGQGDQFSSGFVDINPNSKIPA
                     LVDHSGSTPVRVFESGAILLYLAEKFGAFLPTDPAARAEAYSWLMWQMGSAPFIGGGF
                     GHFYFYAPVKIEYAINRYAMETKRLLDVANQRLAQSPWLAGEDYTIADIAAFPWLGNI
                     VQGRSYGDAATFLSAHDYPHVLRWAETIAARPAVQRGRIVNARDGLKERHSAADIDAF
                     LAAQVATA"
     gene            complement(1219..2319)
                     /locus_tag="VFF98_17365"
     CDS             complement(1219..2319)
                     /locus_tag="VFF98_17365"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZU65460.1"
                     /translation="MASLAEPQSAFTRLPHPAPVDAATREAVLANPGFGTSFTDHMVT
                     IEWTEGKGWHDATIGPRRALSLDPAAAVLHYAQEIFEGLKAYKQADGAIALFRPQANA
                     ARFNASARRLAMPELPEETFVAAVKELVLADKAWIPAQDGASLYLRPFMIATEAFLGV
                     RPAKSYKFIVIASPAGNYFKSGAPAVSIWVSDYTRAAPGGTGAAKCGGNYAASLVPQA
                     EAIARGHDQVVFLDAAEHKWIEELGGMNLYFAFDDGSLITPPLSGTILPGITRDSLLQ
                     LAREEGLAIREERYSIDQWRADAASGRLLETFACGTAAVVTPVGRVCGREGEFAIGSG
                     GPGQLTQKLKDRLVSIQRGTAPDSHGWVVKLG"
     gene            complement(2998..3582)
                     /locus_tag="VFF98_17370"
     CDS             complement(2998..3582)
                     /locus_tag="VFF98_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65461.1"
                     /translation="MTAKPPACKARPPFTPLRAAMALAALLPAACAAPPASHPPAPVP
                     APLPAPPLAPAPQPADWRAAPLSPGRWHWAMEQGRSTARFVAADGRSRFVLACLPGEG
                     AGTITWLYAAPASGHALATPGPQAMFTTFPDGPHPVLSQWQAGGLVGGLGARQALLDA
                     LAFTQGRFVVEIEGMAPLVLPAATEISRVVEDCR"
     gene            3666..4445
                     /locus_tag="VFF98_17375"
     CDS             3666..4445
                     /locus_tag="VFF98_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZU65462.1"
                     /translation="MAEGGRARKVRVDQALVDRGLVESRARAQALVLAGLVFSGETKI
                     AKPGQTIPADAPLEARGRDHPWVSRGGIKLAHALSHFRLDPAGAVAMDIGSSTGGFTD
                     VLLTHGAAHVFAVDSGTNQLAWKLRQDPRVSVLEQTSARLLTPAHITAPCSWVVCDAS
                     FIALAKVLDVPLALAAPCCRLVALIKPQFEVGRAEVGSRGVVRDAALHERVCAEVRAW
                     VEGKGFVVDGIVPSPITGPEGNVEFLISAQRGGETGGGEAG"
     gene            4566..5120
                     /locus_tag="VFF98_17380"
     CDS             4566..5120
                     /locus_tag="VFF98_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZU65463.1"
                     /translation="MSEIASRGQLRAAWWRWVLVLVPGHLLLGFLSARGSGSGPASGW
                     FMVLAKPGLYPPPVVFPLVWSLLYVLMALALAHVAMARSAPGRGAAIIAYGVQLVLNL
                     AWSPVFFGLHRISWALGVIGALALVLAITVALFARVRPLAALLLAPYLAWVLFAGLLN
                     WQFLALNPVADGQPSGVPAVRLQI"
     gene            5231..5488
                     /locus_tag="VFF98_17385"
     CDS             5231..5488
                     /locus_tag="VFF98_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HZU65464.1"
                     /translation="MQSENPLIADFVKLMNSAAGTFVGATREAREAARERFREAFGGL
                     DFVSREEFDAVKALAAAAREEAETLKARVNALEARLDGSQG"
     gene            5496..6146
                     /locus_tag="VFF98_17390"
     CDS             5496..6146
                     /locus_tag="VFF98_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HZU65465.1"
                     /translation="MQIRAAAIVCAARAHGEAGVVARLLTADHGMVATYVAGGRGRDL
                     RPVLIPGNLVEADLYARGGASLPSARLELLASRGPWLTEPLPAAAIGWTCALSAAALP
                     ERQAYPPLYTALAALLDAVCHAPSARGWAPALLAYEGLVLRELGYGRAFGAGGDGAGW
                     LAAARAGGADWPLLMRGFAEMAGLLARYLLADRRGDVMGARALLVDRLARIGVEQI"
     gene            6166..7224
                     /gene="leuB"
                     /locus_tag="VFF98_17395"
     CDS             6166..7224
                     /gene="leuB"
                     /locus_tag="VFF98_17395"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZU65466.1"
                     /translation="MKIAVFAGDGIGPEVTREAVRVLDALEISGLELVEGDVGGAAYY
                     KHGHPLPQATLEIAHRVDGILFGAVGDFSCDHLERHLRPEQAILGLRKELSLFANLRP
                     ARLFPGMDGFSALRPEVANQIDLLIVRELNGDVYFGEKGTRTLADGRRQGWDLMSYAE
                     DEVRRIAHVAFRAAQGRGRKLCSVDKANVLETSQLWRDVVIEVAAEYPDVELSHMYVD
                     NAAMQLVKAPGQFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGEKQTEYGTLGLY
                     EPIHGSAPDIAGQNRANPMATILSAAMLLRHSLGLEAEATCVEGAVAKALADGVRGGD
                     LGGSHTTSEIGDAVLARL"
     gene            7294..>7485
                     /locus_tag="VFF98_17400"
     CDS             7294..>7485
                     /locus_tag="VFF98_17400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZU65467.1"
                     /translation="MMTQSLQLAVVLPTLNERKNLRPLVERLAKALGDIRWEAIIVDD
                     NSRDGTADEARAINLEDPRV"
BASE COUNT         1155 a         2347 c         2746 g         1237 t
ORIGIN      
        1 ctagctgaag cgctgtcaga tttggccgta agtcactgcg cgaaaaccga aatgcgagga
       61 cttaactggc tggggcggga ggattcgaac ctccgcatgg cggtaccaaa aaccgctgcc
      121 ttaccgcttg gctacgcccc aacatccatg ccgattcccg cgcattttcg cgggccggag
      181 cgcgcgctta gccggaagcg gggcgaaagg gaagaggcat tgcgcacgag tgtgccatgc
      241 cccttgccct tttgccgccg ccgatcaggc cgtggccacc tgcgctgcca ggaaggcatc
      301 gatatcggcg gcgctgtgcc gttccttcag cccgtcacgc gcgttgacga tgcgcccgcg
      361 ctgcaccgcc gggcgcgcgg cgatcgtctc ggcccagcgc agcacatgcg gatagtcatg
      421 ggcggaaagg aaagtggccg catcgccata ggagcggccc tgcacgatat tgcccagcca
      481 cgggaaggcg gcgatatcgg cgatggtata gtcttcgccg gccagccagg ggctttgcgc
      541 caggcgctgg ttggccacat cgagcagccg cttggtttcc atcgcatagc ggttgatggc
      601 atattcgatc ttcaccggcg cgtagaaata gaaatggcca aagccgccgc cgatgaaggg
      661 cgcagagccc atctgccaca tcagccagct ataggcctcg gcgcgggcgg cgggatctgt
      721 gggcaggaag gcgccgaatt tttcagcgag ataaagcagg atcgcgcccg attcgaacac
      781 gcgcacaggg gtgctgccgc tgtggtccac cagggcgggg atcttggaat tggggttgat
      841 gtcgacaaag ccggaggaga actggtcacc ctggccgatg ttgatgaacc aggcatcata
      901 ttcggcgccc gcgtggccgg cggccagcag ctcttccagc aggatgccca ctttctggcc
      961 gttgggtgtg gccagcgaat agagctggat cgggtgctgg ccccggggca gggcctcgtc
     1021 atgggtagcg ccggcgatcg ggcggttcag gctgaaaacg ccgtcaccct cgggcttggc
     1081 ccattcccat accttgggcg gggtatattc ttgcgacatg tcaggctcct gaaaggggaa
     1141 ggaaacgggc gatccttccc caagatagga ggctggccag gcaatgccaa gcgcgccgct
     1201 agggtttttg tggcccgctc agcccagctt gacgacccag ccgtggctgt cgggcgcggt
     1261 gccgcgctgg atggagacga gccggtcctt cagcttctgc gtcagctggc cggggccgcc
     1321 gctgccgatg gcaaattcgc cctcgcgccc gcacacgcgg cccaccggcg tcaccaccgc
     1381 ggcggtgccg caggcaaagg tttccagcag ccggcccgag gcggcatcgg cgcgccactg
     1441 gtcgatcgaa tagcgctcct cgcggatggc caggccttcc tcgcgggcca gttgcagcag
     1501 gctgtcccgc gtgatgccgg gcaggatcgt gcccgacagc ggcggcgtga tcaggctgcc
     1561 atcgtcgaag gcgaaataga ggttcatgcc ccccagttcc tcgatccact tgtgctcggc
     1621 cgcatcgagg aacaccacct gatcgtggcc acgggcaatc gcctcggcct ggggcaccag
     1681 gctggcggca taattgccgc cgcatttggc agcgccggtg ccgccggggg ccgcgcgggt
     1741 gtaatcgctc acccagatcg acacggccgg cgcgcccgac ttgaagtagt ttccggccgg
     1801 gctggcgatc acgatgaatt tatacgattt tgccgggcgc acaccgagga atgcctcggt
     1861 ggcgatcatg aacgggcgca gatagagcga ggcgccatcc tgcgccggga tccacgcctt
     1921 gtcggccagc accagttcct tcacggcggc cacgaaggtt tcctcgggca gctcgggcat
     1981 ggccagacgc cgggcgctgg cgttgaagcg ggcggcattg gcctgcgggc ggaacagggc
     2041 aatggcgcca tcggcctgct tgtaggcctt cagcccttcg aagatttcct gcgcataatg
     2101 gagcacggcc gccgccggat cgagcgacag cgcccggcgc gggccgatgg tggcatcgtg
     2161 ccagcccttg ccctcggtcc actcgatcgt caccatatga tcggtgaagc tggtgccgaa
     2221 gccggggttg gccaataccg cctcacgcgt ggcggcatcc accggcgcgg gatgaggaag
     2281 gcgggtgaag gctgactggg gctcggcgag gctcgccatg gggcaggatc tccgttggcg
     2341 caggcgctgt gaaaacaaga ttgccttgtg aaacaagatt gcttttcagg agccatgcgc
     2401 gaaataatcg aacacaggct attatcctta gcagtttgcc gcgcgcgggc aagagcggcc
     2461 ggcccggaaa aagccgccga ttggcctgaa aaaccatgca ggaggcagga aaaggggcgc
     2521 ggatcgggcc aaatgccttc gcgccgcagc aggggcatcg atcgggaaag ggccatcggg
     2581 aaaagggggc acgcaaatga gaagggccgc cttccggttg tccggtaagc ggcccttcgc
     2641 atggtcagcg gccggaagtg ccgcccacga agcctttatc gctcaaatcg taaaatcagc
     2701 gataatgctc cgcgctgcgg tttgccgacc tctctgctga accatgagac atcggatcac
     2761 ctcctttcgc gctgttgagc caagcccgtg cgcccgcttg cagatcgccc ggggtgttcg
     2821 gggcgggccc tgtgggcact gcatcccgtt cacatttgca aggtgcccta ttcggcactg
     2881 cacaacaagt cttgcaaagg atttttttgc cgtcagaatg gcggctttcc ggggttctcc
     2941 acagccacct gcgggcgcct tccggcgtct gtccgggcag gtggcgcggc ggggttttca
     3001 gcggcaatcc tccaccaccc ggctgatctc ggtggcggcc ggcagcacca gcggcgccat
     3061 cccctcgatc tccaccacga agcggccctg ggtgaaggcc agcgcatcca gcagcgcctg
     3121 gcgcgcgccc agcccgccca ccaacccgcc ggcctgccac tggctgagca ccgggtgggg
     3181 gccatcgggg aaggtagtga acatcgcctg cgggccgggc gtggcaaggg cgtggccgct
     3241 ggctggcgcg gcatagagcc aggtgatcgt gccggcgcct tcccccggca ggcaggccag
     3301 cacgaagcgg ctgcggccat ctgcggcgac aaagcgcgcg gtcgatcggc cctgctccat
     3361 cgcccagtgc cagcggccgg ggctgagcgg ggcggcgcgc cagtctgccg gctggggggc
     3421 aggggccagg ggcggcgcgg gcaggggggc gggcacgggc gccggcgggt ggctggcggg
     3481 cggcgcggcg caggcggccg gcaacagggc ggccagcgcc atggcggcgc gcaggggcgt
     3541 gaaagggggg cgggccttgc aggcgggcgg ttttgccgtc atggcacttc ctatggaagc
     3601 tgcgcgccct gcaaacaacc ccgcaaacgc cccccgctgc cctgcccggg gcctggcggt
     3661 gcgccatggc tgagggcggg cgggccagga aggtgcgggt tgatcaggcg ctggtcgatc
     3721 gcgggctggt ggaaagccgc gcgcgggcgc aggcgctggt gctggcgggg ctggtgtttt
     3781 ccggcgagac gaagatcgcc aagcccggcc agacgatccc cgccgatgcc ccgctggaag
     3841 cgcgcgggcg cgatcatccc tgggtcagcc gggggggcat caagctggcc catgcgctca
     3901 gccatttccg gctcgatccg gcaggcgcgg tggcgatgga tatcggcagt tccaccggcg
     3961 gcttcaccga tgtgctgctg acgcatgggg cagcgcatgt ctttgcggtt gattcgggca
     4021 ccaaccagct ggcctggaag ctgcggcagg acccgcgcgt gagcgtgctg gagcagacca
     4081 gcgcccgcct gctcaccccg gcgcacatca ccgccccctg cagctgggtg gtgtgcgatg
     4141 ccagcttcat tgcgctggcc aaggtgctgg acgtgccgct ggcgctggcc gcgccctgct
     4201 gccggctggt ggcgctgatc aagccgcagt tcgaggtggg ccgggccgag gtgggcagcc
     4261 gcggcgtggt gcgcgatgcg gccctgcacg aaagggtgtg cgccgaggtg cgcgcctggg
     4321 tggagggcaa gggctttgtg gtggatggca tcgtgcccag ccccatcacc gggccggagg
     4381 gcaatgtgga attcctgatc tcggcccagc gcggtgggga aacagggggc ggagaagcgg
     4441 gttaaccgtc ccggcctggg gcattttgcc cgcgccagag ggttcaaacc gcgctaggag
     4501 aaggataaca gggcgccatt gacgccggaa tcgcgttttt cgaaaggctg tggggcttga
     4561 gacggatgag cgagattgct tccagggggc agttgcgcgc ggcctggtgg cgctgggtgc
     4621 tggtgctggt gccgggccat ttgctgctgg gcttcctgtc ggcgcggggc agcggcagcg
     4681 ggcctgccag cggctggttc atggtgctgg ccaagccggg cctctatccg ccgccggtgg
     4741 tgttcccgct ggtatggagc ttgctgtatg tgctgatggc gctggcgctg gcgcatgtgg
     4801 cgatggcccg ctcggcgccg gggcgcgggg cggcgatcat cgcctatggc gtgcagctgg
     4861 tgctgaacct ggcctggtcg ccggtgttct ttggcctgca tcgcatcagc tgggcgctgg
     4921 gggtgatcgg cgcgctggcg ctggtgctgg cgatcacggt ggcgctgttt gcccgggtgc
     4981 ggccgctggc ggcgctactg ctggcgccct atctggcctg ggtgttgttt gccggcctgc
     5041 tcaactggca gttccttgcg ctgaaccctg tggccgatgg ccagccttcc ggcgtgccgg
     5101 cggtgcgcct gcagatatag gcctattccc cggccgatcc catctcgcac cttgccagca
     5161 gggcgcagcg gcgccatcta ggcggccgta gcaaccggcc ccccggggcc ccgcaaaacg
     5221 gatcattgcc atgcagagcg aaaaccccct gatcgccgat ttcgtcaagc tgatgaacag
     5281 cgccgccggc actttcgtgg gggccacgcg tgaggcgcgc gaggccgcgc gcgagcgctt
     5341 ccgcgaggcc ttcggcgggc tcgatttcgt cagccgcgag gagtttgacg cggtgaaggc
     5401 actggccgcc gccgcgcgcg aggaagccga gacgctgaag gcccgcgtga acgcgctgga
     5461 agcccggctg gacggcagcc agggctgagc ctttcgtgca gatccgcgct gccgccatcg
     5521 tctgtgccgc gcgcgcgcat ggcgaggcgg gggtggtggc gcgcctgctc acggccgatc
     5581 acggcatggt ggcgacctat gtggcgggcg ggcgcgggcg cgatctgcgg ccggtgctca
     5641 ttcccggcaa tctggtcgag gccgatctgt atgcccgggg cggggccagc ctgccctcgg
     5701 cccggctgga gctgctggcc agccgcggcc cctggctgac agaacccttg ccggccgccg
     5761 ccatcggctg gacctgcgcg ctgagcgccg ccgccctgcc cgaacggcag gcctatccgc
     5821 cgctctacac ggcgctggca gcccttctgg atgcggtgtg ccatgcccct tcggcgcggg
     5881 gctgggcgcc ggcgctgctg gcctatgaag ggctggtgct gcgcgagctg ggctatggcc
     5941 gggcctttgg cgcgggcggt gatggcgccg gctggctggc cgcggcgcgg gctggcgggg
     6001 cggattggcc cctgctgatg cgcggttttg cggaaatggc ggggctgctt gcgcggtatc
     6061 tgcttgctga tcggcgcggc gatgttatgg gagcgcgagc gctgctggtc gacaggctgg
     6121 cgcgcatcgg tgttgaacag atttagaagc aggctcaggc aatccatgaa gatcgcggtt
     6181 ttcgctggtg acggcatcgg cccggaagtg acgcgcgagg cggtgcgggt gctcgacgcg
     6241 ctcgagatct ccggcctcga actggtggaa ggcgatgtgg gcggcgcggc ctactacaag
     6301 cacggccacc cgctgcccca ggccacgctg gaaatcgcgc atcgggtgga tggcatcctg
     6361 ttcggcgcgg tgggcgattt ctcgtgcgac catctggagc gccacctgcg cccggaacag
     6421 gccattctgg gcctgcgcaa ggaactgtcg ctgtttgcca acctgcgccc ggcgcgcctg
     6481 ttcccgggga tggacggctt ttccgccctg cgccccgagg ttgccaacca gatcgacctg
     6541 ctgatcgtgc gcgagctgaa cggcgatgtc tatttcggcg agaagggcac ccgcacgctg
     6601 gccgatggcc gccgccaggg ctgggacctc atgagctatg ccgaggatga ggtgcggcgc
     6661 attgcccatg tcgccttccg cgcagcgcag gggcgcggcc gcaagctgtg cagcgtggac
     6721 aaggccaatg tgctggaaac cagccagctg tggcgcgatg tggtgatcga ggtcgccgct
     6781 gaatatcccg atgtcgagct cagccacatg tatgtggaca atgccgcgat gcagctggtg
     6841 aaggcgccgg ggcagttcga tgtggtggtc accggcaatc tgttcggcga tatcctgtcg
     6901 gatcaggcca gcatgtgcgt gggctcgatc ggcctcctcg cctcggcctc gctgggcgag
     6961 aagcagaccg aatatggcac gctgggcctt tatgagccga tccacggcag cgcgcccgac
     7021 atcgccggcc agaaccgggc gaacccgatg gccacgatcc tgtcggccgc gatgctgctg
     7081 cgccacagcc tggggctgga agcggaagcg acctgcgtgg aaggcgcggt ggccaaggcg
     7141 ctggccgatg gcgtgcgtgg cggcgatctg ggcggcagcc acaccaccag cgagatcggc
     7201 gatgcggtgc tggcgcggct ctgaacgcgc cttgccgcaa agcgcggcgg gggcctgcgc
     7261 atgcatgtgg acgataaaag gacgaatgtc ggcatgatga cccaaagcct gcaactggct
     7321 gtggtgctgc ccacgctgaa tgaacgcaag aatttgcgcc cgctggtcga gaggctggcg
     7381 aaggcgctgg gcgatattcg ctgggaggcg attatcgtgg acgacaacag ccgggatggc
     7441 acggccgatg aggcgcgggc gatcaatctg gaagatccgc gggtg
//
LOCUS       DASRYE010000335         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_68652,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000335 DASRYE010000000
VERSION     DASRYE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68652"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..800
                     /gene="dcd"
                     /locus_tag="VFF98_17405"
     CDS             234..800
                     /gene="dcd"
                     /locus_tag="VFF98_17405"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZU65468.1"
                     /translation="MAILSDKWIRAQALAHGMIEPFVERQTRNGEGGGIISYGLSSYG
                     YDARVADEFKIFTNVDSAVVDPKDFAANSFVDRKTDVCVIPPNSFALARTVEYFRVPR
                     DVLVICLGKSTYARCGIIVNVTPLEPGWEGHVTLEFSNTTPLPAKIYANEGACQFLFL
                     QGNEPCEVSYADRAGKYMGQRGVTLPRL"
     gene            complement(836..1549)
                     /gene="ung"
                     /locus_tag="VFF98_17410"
     CDS             complement(836..1549)
                     /gene="ung"
                     /locus_tag="VFF98_17410"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZU65469.1"
                     /translation="MAEEGTAAGRTAAGALPASWQGALAPALATPAARRLGGFLKGEE
                     AAGKRIFPPQGTRLAALELTPLDSVKVVILGQDPYHGAGQAHGLAFSVQEGVRVPPSL
                     VNIYKELETDLGLPRAAHGDLSGWARQGVLLLNNALTVEEGRAGSHQNLGWEAITDAA
                     VAAVAAGAQPCVFLLWGAHAQKKAARVPGLATGPHLVLKAPHPSPLSAHNGFFGCRHF
                     SQANAFLESHGRGAIDWRV"
     gene            1617..2240
                     /locus_tag="VFF98_17415"
     CDS             1617..2240
                     /locus_tag="VFF98_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZU65470.1"
                     /translation="MAVYLHEEDLPADVLGPGPLAVDTETMGLITRRDRLCLVQIADG
                     RGDEHLVRFGPGSAYAAPNLKAALADPARLKIYHFARFDIAAIQHYLGVSATPAFCTK
                     IASKLVRTYTDRHGLKDLVRELLGKDISKQQQSSDWGGAELSDAQKDYAASDVRYLHA
                     CHAILVDRLAREKRTELAQACFDFLPHRVALDLAGWPEHDIFSHEAP"
     gene            2250..2897
                     /gene="lptC"
                     /locus_tag="VFF98_17420"
     CDS             2250..2897
                     /gene="lptC"
                     /locus_tag="VFF98_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZU65471.1"
                     /translation="MSETAASMRDRRRAWALPGGNHDRWVGRLARGLPAAVGMIVAAM
                     VVTPLFPRGDVSFLLDRNKVAITEDRLQVSGATYRGEDDEGRPFWLTAGRAVQHSAHV
                     ARVQMEQLVAHLQMADGLAQVTADRGTYDLTQEQMQLQGPVNISRQDGYRILTNDVSI
                     DVKHRRAYGESDVRGTLPEGHFTADRMTADLAEKRVVLEGHAHLRMSQSRQPRKP"
     gene            2999..3583
                     /locus_tag="VFF98_17425"
     CDS             2999..3583
                     /locus_tag="VFF98_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HZU65472.1"
                     /translation="MSRPNASPARPFRLAPGLRALALVGLLTGSAAALAAGLSGHDSD
                     APVNYSADRIEVMDKDHRVLLTGNVDITQDELRLRSDRALVAYTNSGGSDGEGVKIQR
                     LDATGHVIVTRRDQTATGDVGTYDFNSRIITMVGNVELHRGTESSRGGRLVIDLDKST
                     SNFVGAGGKLTNGSSAPGRVSGTFSVKKKDKAAQ"
BASE COUNT          604 a         1291 c         1198 g          555 t
ORIGIN      
        1 tcgcgcgcct cgccgagggc gatccgctgg gcgaggtgga gcgctggctg gccgcccatg
       61 agccggccgc cgaacgcctg cgcctgctgg ccgcccgcgc ccggcaggcg ccgcgcctct
      121 cgccggccat gctggcgcag atcgcccggc aggcgcgcat cctgctggcg ccctaggggg
      181 gccgggcggg ccccctcttg ccggcgcggg aaccttgcgc catgaacgcg cccatggcta
      241 ttctatccga caagtggatc cgcgcgcagg cgctcgcgca tggcatgatc gaacccttcg
      301 tggagcggca gacccgcaat ggcgagggcg gcggcatcat ctcctatggc ctctcctcct
      361 acggctatga cgcgcgggtg gccgacgagt tcaagatctt caccaatgtc gattcggccg
      421 tggtggaccc gaaggacttt gccgccaaca gcttcgtcga tcgcaagacc gatgtctgcg
      481 tgatcccgcc caattccttc gcgctggccc gcacggtgga atatttccgc gtgccgcgcg
      541 atgtgctggt catctgcctg ggcaagagca cctatgcccg ctgcggcatc atcgtgaacg
      601 tgacgccgct ggagccgggc tgggaaggcc atgtgacgct ggaattcagc aacaccacgc
      661 cgctgcccgc caagatctat gccaatgagg gcgcctgcca gttcctcttc ctgcagggga
      721 acgagccctg cgaggtgagc tatgccgacc gcgccggcaa atatatgggc cagcgcggcg
      781 ttaccctgcc caggctgtaa aaggggccca gactataaag aggcgccgcg cgcccttaca
      841 cgcgccagtc gatcgcgccg cgcccgtggc tttccagaaa ggcgttggcc tggctgaaat
      901 ggcggcagcc gaaaaagccg ttatgcgccg atagcgggct ggggtgcggc gccttcagca
      961 ccagatgcgg cccggtggca aggccgggca cgcgcgccgc cttcttctgc gcatgggcgc
     1021 cccacagcag gaacacgcaa ggctgcgcac cggccgccac cgccgccacg gcggcatcgg
     1081 taatcgcttc ccagcccagg ttctggtggc tgccggcgcg cccctcttcc accgtcagcg
     1141 cattgttgag cagcagcacg ccctgccgcg cccagccgga cagatcgcca tgggcagcac
     1201 gcggcaggcc cagatccgtc tccagctcct tgtagatgtt gacgaggctg ggcggcaccc
     1261 gcacgccctc ctgcaccgaa aaggccagcc catgcgcctg gcccgcgcca tgatagggat
     1321 cctgccccag gatcaccacc ttcacgctat ccagcggggt cagctccagc gcggcaaggc
     1381 gcgtgccctg gggcgggaag atgcgcttgc cggccgcttc ctcgcccttc aggaagccgc
     1441 ccagccggcg cgcggcgggc gttgccagcg ccggtgccag cgccccctgc cagctggccg
     1501 gcagcgcccc tgccgccgtg cgccctgctg ccgtgccttc ctctgccatg caaaagcctc
     1561 caaacgcatt ggggggcttt tcaccccgcg cgggaaagcc taagcacgtc ggcgcaatgg
     1621 ctgtctatct ccacgaagaa gatctccccg ccgatgtgct ggggcccggg ccgctcgcgg
     1681 tcgacactga aaccatgggg ctgatcaccc ggcgcgacag gctgtgcctc gtgcagatcg
     1741 ccgatggcag gggggatgag catctcgtgc gctttggccc cggcagcgcc tatgccgcgc
     1801 ccaacctcaa ggcggcgctg gccgatcctg cccggctgaa gatctatcac ttcgcccgct
     1861 tcgacatcgc cgccatccag cattacctcg gcgtttccgc cacgcccgcc ttttgcacca
     1921 agatcgccag caagctggtg cgcacctaca ccgaccggca tggcctgaag gatctggtgc
     1981 gcgaactgct cggcaaggat atttccaagc agcagcagtc gagcgactgg ggcggggcgg
     2041 aactgtcgga cgcgcagaag gattatgccg cctccgacgt gcgctatctg catgcctgcc
     2101 acgccattct ggtcgatcgg ctggcgcgcg agaagcgcac cgaactggcc caggcctgct
     2161 tcgatttcct gccgcaccgg gtggcgctgg atctggccgg ctggcccgaa cacgacatct
     2221 tcagccacga agccccctaa gccacagcaa tgagcgaaac cgccgcctcc atgcgcgatc
     2281 gccggcgcgc ctgggcgctg cccggcggca accacgatcg ctgggtgggc cggctggcgc
     2341 gcggcctgcc ggcggcggtg ggcatgatcg tggcggccat ggtcgtcacc ccgctgttcc
     2401 cgcgcggcga tgtcagcttc ctgctggatc gcaacaaggt ggcgatcacc gaggaccgcc
     2461 tgcaggtgag cggcgccacc tatcgcggcg aggacgacga gggccgcccc ttctggctca
     2521 ccgccgggcg cgcggtgcag cattcggccc atgtcgccag ggtgcagatg gagcagctgg
     2581 tggcccacct gcaaatggcc gacgggctgg cccaggtgac cgccgatcgc ggcacctacg
     2641 acctgacgca ggagcagatg cagttgcagg gcccggtcaa catcagccgg caggatggct
     2701 atcgcatcct caccaatgat gtgagcatcg atgtgaagca ccgccgcgcc tatggcgaga
     2761 gcgacgtgcg cggcaccctg cccgaaggcc atttcaccgc cgaccggatg accgccgacc
     2821 ttgccgaaaa gcgcgtggtg ctggaaggcc atgcccatct gcgcatgagc cagagccgcc
     2881 agccccgaaa gccctgaaca ggcggcccga aacaggcttt gccaatgccc cgcaacaggc
     2941 tgtgccgaaa aacgcgcggg tggtttcccc cggccgcaaa accttctaag ggaggcgcat
     3001 gtctcgcccg aacgcttcgc cggcccgtcc tttccgtctt gctccgggcc tgcgcgccct
     3061 tgcccttgtc ggcctgctga cgggcagcgc ggcggcgctg gcggccggcc tttccggcca
     3121 tgacagcgat gccccggtga attattccgc cgatcgcatc gaggtgatgg acaaggacca
     3181 ccgcgtgctg ctcaccggca atgtcgatat cacccaggac gagctgcgcc tgcgctcgga
     3241 ccgggcgctg gtggcctata ccaacagcgg cggcagcgat ggcgaaggcg tgaagatcca
     3301 gcggctggat gccaccggcc atgtcatcgt cactcgccgc gaccagaccg cgacgggcga
     3361 tgtcggcacc tatgatttca acagccgcat catcaccatg gtgggcaatg tggaactgca
     3421 ccgcggcacc gaaagttcgc gcggggggcg gctggtgatc gacctcgaca agagcacctc
     3481 gaacttcgtg ggcgccggcg gcaagctcac caatggcagt tcggcgccgg gccgggtgag
     3541 cggcactttc tcggtgaaga agaaggacaa ggcggcgcag taagccgcct agccggcagc
     3601 gcgcgcggcc acgcgggcaa tctcggtcag gccctgggcc agcagcag
//
LOCUS       DASRYE010000336        10725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_175204,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000336 DASRYE010000000
VERSION     DASRYE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175204"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..800
                     /locus_tag="VFF98_17430"
     CDS             120..800
                     /locus_tag="VFF98_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZU65473.1"
                     /translation="MTDAAASLTAAARLADINARIAAAARLARRAPADVRLIAISKTH
                     EAEAIQPLIDAGQRLFGENRVQEAAAKWPPLRAAHDGIELHLVGQLQSNKAEEAVALF
                     DCIHAVDRPSLVAALAKAMDRQAKRVPCFVQVNIGDEPQKGGCAVADLPALLAEARAA
                     GLPLAGLMCVPPAGIEAAPFFALLAKLAADNGLEGLSMGMSEDFETAVTLGATCVRVG
                     SALFGARG"
     gene            800..1951
                     /locus_tag="VFF98_17435"
     CDS             800..1951
                     /locus_tag="VFF98_17435"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZU65474.1"
                     /translation="MAGLLAAQAADLADLAARHRLRALAPQAGVDFASNDYLGLATSG
                     ALAEAAAAALARGVPVGSGGSRLLRGNHAEHEALEEEAARFFGGEAALFLPTGFTANS
                     ALIATLPQREDRIFADALIHASTHEGLRLTRAPYELVPHGDAGAFDAAITRWRAEGGR
                     GTPWLCVESLYSMDGDLAPLVDLAAVAARHEALLVVDEAHATGLFGPGGRGCVAALPD
                     RSRIISLHTLGKALGCEGALLVGPAVMRQFLVNRARGFIFSTAPSPLNAAIARAALAL
                     VAGADDLRAALFARIDQARGLLAPLGAECHGTPILPLVLGADERAMAVAGACQRAGFD
                     VRGIRPPTVPEGSARLRIVITLNAGEAEIAALATTLATTLAATLKEVRA"
     gene            1948..2592
                     /gene="bioD"
                     /locus_tag="VFF98_17440"
     CDS             1948..2592
                     /gene="bioD"
                     /locus_tag="VFF98_17440"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZU65475.1"
                     /translation="MKGLIVTGTDTGIGKTMLSAGLISVLQAHAGGARYWKPVQSGLE
                     EETDSEAVARLAPGVAVHPEAYRLLTPASPHLAAFIDGVEIDPAALALPEGTAPLVVE
                     GAGGALVPLSETLLYADLFARWGLPVVVAARTGLGTINHSLLTIEALRARQVPIAGIA
                     FVGEAHPENERIIPAIGGVKRLGRLPWLEEITPVELAGAMATHFNIPALLEALS"
     gene            2589..3848
                     /locus_tag="VFF98_17445"
     CDS             2589..3848
                     /locus_tag="VFF98_17445"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZU65476.1"
                     /translation="MSGGAIWHPFTQHGLGEPIPEVVRAEGAVLHTADGARVVDAISS
                     WWVITHGHCHPRIVAAIGEQAARLDQVIFAGWTHRPAEVVAAGLVGLTGLPYAFFSDS
                     GSTAVEVALKMALGHWVHRGEGHRHRILVMQHSYHGDTIGAMSVGERGVYNAPYAPLL
                     FDVGVIPFPHAGAEQATYDALEQACAAGDVAAFIVEPLVLGAGGMLVYPPQVLAGMRA
                     ICAAHGVLFIADEVMTGWGRTGTLLACEQAGITPDILCLAKGLTGGSIPLAVTMASAD
                     IYASHYSTDRAKTFYHSSSYTANAIACAAAAANLGIWQEEPVREGIATLAARQAQGAA
                     MLAAVPGVGHVRQCGTILALDYEVGAGAGYLSALQPRLLAFFREAGLLIRPLGNTAYL
                     MPPYSISAEDLARCHAALAEAAALAKG"
     gene            complement(3884..5347)
                     /gene="pyk"
                     /locus_tag="VFF98_17450"
     CDS             complement(3884..5347)
                     /gene="pyk"
                     /locus_tag="VFF98_17450"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZU65477.1"
                     /translation="MIKLNPRGRKVKILSTLGPASREPEMIGKLIRAGADAFRVNMSH
                     GDQATHAQTIAHIRAAEKQFGRPIAILADLQGPKLRVGTFTEGRKVIRHGSHFTLDRD
                     PTPGDETRVCLPHPELFGILQKGQRLLIDDGKLRLRVIRADADAILCSAEVGGVISDR
                     KGVNVPDAVVPVPALTEKDRRDLAFAVDQQVDYLGLSFVQRPEDVAEARKLIGPNGPA
                     IIAKIEKPAAIGRLDEIIELADGIMVARGDLGVELHPEEVPPLQKRIVEATRRQGKPV
                     IVATQMLESMIESPAPTRAEVSDVANAVYDGADVVMLSAETASGQWPEEAVSIMHRIA
                     SQVEGDTYYRQRVHLAEINPDHTTADALAKASSAIADTLTIAGIVVFTGSGSTARRVA
                     RERPSAPMLVLTPSLKTARKAALLWGAHAVHTKDIGSFEEMIAKGKRMALRHGFGEAG
                     SKLITLAGVPFGTPGATNLLHVVTLKGDELKNYKLED"
     gene            5418..5744
                     /locus_tag="VFF98_17455"
     CDS             5418..5744
                     /locus_tag="VFF98_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HZU65478.1"
                     /translation="MNKNVSSPAADALDGLDDKVAAAAFRRLVRHLQHRHDAQNIDLM
                     GLAGFCRNCLADWIEEAGAGLGKAGAREVIHGLAAEEWKRRYQSPATPEQLARMEESL
                     KKNAAG"
     gene            5867..6115
                     /locus_tag="VFF98_17460"
     CDS             5867..6115
                     /locus_tag="VFF98_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HZU65479.1"
                     /translation="MADNDAQAADERLRLLIERIERLEEEKKGIGDDIKDVYLEAKSA
                     GYDPKAMRQVVRIRKMKPDDRKEMEAILDVYLTALGIS"
     gene            6331..7095
                     /locus_tag="VFF98_17465"
     CDS             6331..7095
                     /locus_tag="VFF98_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZU65480.1"
                     /translation="MAGHSKFKNIMHRKGAQDKKRSAQFSKLSREITVAAKMGMPDPD
                     MNPRLRAAINAAKAQSMPKDNIARAIDKASRGDGENYEEVRYEGYGPGGVAIIVEALT
                     DNRNRTATNVRTAFAKNGGNLGASGAVSHGFDRLGLIEYPASVGDEDKVLEAAIEAGA
                     EDVESDMGDGDENPGSHQIWVAVEELHPVARELEKVLGEAEGVKLAWRPTLKTAVDAD
                     TAATLLKLIDVLEEDDDVQTVWGNYDIPEDVLEKLA"
     gene            7095..7451
                     /locus_tag="VFF98_17470"
     CDS             7095..7451
                     /locus_tag="VFF98_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HZU65481.1"
                     /translation="MISGALIARALLSGAIIAAIAEIGKRAPTFAALVASLPLVSVLG
                     MVFLWQARPDAANMAAHAEATFWYVLPSLPMFLLIPALLRQGVGFWPALGLGCALTVA
                     LYLGLARLAPRWGIFL"
     gene            7471..7959
                     /gene="ruvC"
                     /locus_tag="VFF98_17475"
     CDS             7471..7959
                     /gene="ruvC"
                     /locus_tag="VFF98_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZU65482.1"
                     /translation="MVILGVDPGLTCTGWGIVAKAGSRISHIANGQIRTDVAAPMAER
                     LLHLDRELTDVIARHHPDTGAVEEIFVNVNPQSTLKLGQARGVALLSLARGGLDVAEY
                     PTKNIKKALVGTGNAEKQQVQHMLKILLPGVKLAGADAADALAVAITHAHMMPARPLA
                     AG"
     gene            complement(7963..9423)
                     /locus_tag="VFF98_17480"
     CDS             complement(7963..9423)
                     /locus_tag="VFF98_17480"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65483.1"
                     /translation="MLLGVALRIWAFSPLDTLHPDELMQYFEQANRLVTGHGTVPWEY
                     RYGIRSWLLPQLLAGPVALGHWLAPGTLAPYLAARIAFAALALISLPAAWRLGLLSGR
                     REGLMALFVVAVWFESVLFGELLLSETLAAALMLLAAALLLADAPGRRALQAAGLLLG
                     LGVVFRMQYAVFAAVLAAMALKADWRRWRWLIEGGLAALALGALSDLAMGRAPFLWLW
                     RYYAMNVGDGRASIFGVSPPYAFVIVLLIHLGPAVLPILAAMFGAGRRYLPLLVAAWA
                     NIAAHSFIPHKEFRFIWLSLLSLLVLGAIGSVRGARWAQGRLGTASPTAAKTGSWLWL
                     ALFPAWWAASFGAQQVVENGAPLRKGGALAGMVIAADRQPGICGLAVPYLWRHYVVDG
                     MLPRPLPLYLGPPIVSQYDTRPIDTIPVADRPPADLLGAANAIVMPSPGPVPQGYARQ
                     RCDTSGKDSMCLYTRPGSCTPAPRWGYQAVLQRLDM"
     gene            9637..10257
                     /gene="ruvA"
                     /locus_tag="VFF98_17485"
     CDS             9637..10257
                     /gene="ruvA"
                     /locus_tag="VFF98_17485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZU65484.1"
                     /translation="MIAKLTGLLDDFGPDWALIDVGGVGYLVQCSARTLHALGLRGER
                     VVVHTEMQVSETDMRLIGFASAGERVWFRLLTAVQGVGSKVALAILSALTVEELQKAC
                     AHGDAAMVARANGVGPKLAARIVNELKDKAGAMAFAGGPGVVLAGGGGGLAGEGGRLA
                     DVVSALQNLGFKPAVAAQAAGAALEELGEEAGEGALIRAALKKAAG"
     gene            10259..>10725
                     /locus_tag="VFF98_17490"
     CDS             10259..>10725
                     /locus_tag="VFF98_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZU65485.1"
                     /translation="MSNPLLSAHAQIEDMDAALRPKTLAEFVGQQAAKENLRIFIESA
                     KSRQEALDHTLFFGPPGLGKTTLAQIIARELGVNFRATSGPVIAKSGDLAALLTNLEH
                     GDVLFIDEIHRLNPVVEEVLYPAMEDRALDLMIGEGPSARSVRIDLPRFTLVGA"
BASE COUNT         1685 a         3616 c         3770 g         1654 t
ORIGIN      
        1 cgcgcggcag gcgggcaatg gcggcgggca gcacggcatc gttgcgggca tcggagacga
       61 gccacaggcg gggcagttcg gggtggcgtt ggcgcatcgc cgcgctatag caccggccca
      121 tgaccgacgc agccgcttct ctcaccgccg ccgcccggct ggccgatatc aacgctcgca
      181 tcgcggccgc cgcccggctt gcccgccgcg cgcccgccga tgtgcggctg atcgccatct
      241 cgaaaaccca tgaggccgag gccatccagc cgctgatcga tgccggccag cgcctgttcg
      301 gcgagaaccg cgtgcaggag gcagccgcca aatggccgcc cttgcgcgcc gctcatgacg
      361 ggatcgaact gcatctggtg ggccagttgc aatcgaacaa ggcggaagag gcggtggccc
      421 tgttcgattg catccatgcg gtggaccggc ccagcctggt ggcggccctg gccaaggcga
      481 tggaccggca ggcgaagcgc gtgccctgct tcgtgcaggt gaacattggc gatgagccgc
      541 agaagggcgg ctgcgcggtg gccgatctgc ccgccctgct ggccgaggcg cgcgccgccg
      601 gcctgccgct ggccgggctg atgtgcgtgc cgccggccgg gatagaggcc gcccccttct
      661 tcgccctgct ggcgaaactg gcggcggata acgggctgga gggcctttcc atgggcatga
      721 gcgaggattt cgaaaccgcc gtcacgctgg gcgccacctg tgtgcgggtg ggcagcgcgc
      781 tgttcggggc gcggggctga tggccggcct gctggcagcg caggcggccg atctggccga
      841 tctggccgcc cgccaccggc tgcgggcgct ggcgccgcag gcgggggtgg attttgcctc
      901 caacgactat ctgggcctgg ccaccagcgg cgcgctggcg gaagccgcgg ccgcggcgct
      961 ggcgcgcggc gtgccggtgg gctctggcgg ctcgcggctg ctgcgcggca accatgccga
     1021 gcatgaggcg ctggaagagg aagccgcccg cttcttcggc ggcgaggcgg cgctgttcct
     1081 gcccaccggc ttcaccgcca attccgccct catcgccacc ctgccccagc gcgaggacag
     1141 gatctttgcc gatgccctga tccatgccag cacgcatgag ggcctgcgcc tcacccgcgc
     1201 gccctacgaa ctggtgcccc atggcgatgc cggcgctttt gatgcggcga tcacccgctg
     1261 gcgggccgag ggcgggcggg gcacgccgtg gctctgtgtc gaatcgctct attccatgga
     1321 tggcgatctg gcgccgcttg tggatctggc cgcggtggcc gcccgccacg aggcgctgct
     1381 ggtggtggac gaggcccatg ccaccggcct gttcggcccc ggcgggcgcg gctgcgtggc
     1441 ggccttgccc gatcgctcgc gcatcatcag cctgcacacg ctgggcaagg cgctgggctg
     1501 cgaaggggcg ttgctggtgg ggccggcggt gatgcggcaa ttcctcgtca accgggcgcg
     1561 cggcttcatc ttttccaccg cgccctcgcc gctcaatgcg gcgattgccc gcgccgccct
     1621 ggcgctggtg gcaggggccg atgatctgcg cgcggcgctt tttgcccgca tcgatcaggc
     1681 gcgcggcctg ctggcgccgc tgggggctga atgccacggc acgccgatcc tgccgctggt
     1741 gctgggggca gacgaacgcg ccatggccgt ggccggcgcc tgccagcgcg ccggtttcga
     1801 tgtgcgcggc atccgcccgc ccaccgtgcc cgaaggctct gcgcggctgc gcatcgtcat
     1861 cacgctgaac gccggcgagg ccgagattgc cgcccttgcc accacccttg ccaccaccct
     1921 ggccgcaacg ctgaaggaag tgcgcgcatg aaaggcctga tcgtcaccgg caccgacacc
     1981 ggcattggca agaccatgct ttcggctggc ctcatttccg tgttgcaggc ccatgccggc
     2041 ggcgcccgtt actggaagcc ggtgcaatcc gggctggagg aggagaccga cagcgaggcg
     2101 gtggcgcgcc tggcgccggg cgttgcggtg cacccggaag cctatcggct gctgaccccg
     2161 gccagccccc atctcgccgc ctttatcgac ggggtggaga ttgaccctgc cgcgctggcc
     2221 ctgcccgaag gcaccgcgcc gctggtggtg gaaggggccg gcggcgcgct ggtgccgctc
     2281 agcgaaacgc tgctctatgc cgatctgttc gcgcgctggg ggctgccggt ggtggtggcc
     2341 gcgcgcacgg gccttggcac catcaatcac agcctgctca ccatcgaggc gctgcgcgcg
     2401 cgccaggtgc cgatcgccgg catcgccttt gtcggcgagg cgcatcccga gaacgagcgg
     2461 atcatcccgg ccatcggcgg ggtgaagcgg ctgggccggc tgccctggct ggaggagatc
     2521 acgccggtgg agctggccgg ggccatggcc acccatttca acattcccgc cctgctggag
     2581 gcgctgtcgt gagcgggggg gcgatctggc atcccttcac ccagcatggc ctgggcgagc
     2641 cgattcccga agtggtgcgc gccgaagggg cggtgctgca cacggccgat ggcgcgcggg
     2701 tggtggatgc gatttccagc tggtgggtga tcacccatgg ccattgccac ccccgcatcg
     2761 tggcggcgat tggcgagcag gccgcccgcc tcgatcaggt gatcttcgcc ggctggacgc
     2821 atcgcccggc cgaggtggtg gccgccggcc tcgtcgggct caccggcctg ccctatgcct
     2881 tcttctccga ttccggctcc accgctgtgg aagtggcgct gaagatggcg ctgggccatt
     2941 gggtgcaccg gggcgagggg caccgccacc gcatcctcgt catgcagcat tcctatcatg
     3001 gcgacacgat cggcgcgatg agcgtgggcg agcgcggcgt ctataacgcg ccctatgcgc
     3061 cgctgctgtt cgatgtgggg gtcattccct tcccccatgc cggcgccgaa caggccacct
     3121 atgacgcgct ggagcaggcc tgcgcggcgg gcgatgtggc cgcctttatc gtcgagccgc
     3181 tggtgctggg ggcgggcggc atgctggtct atccgccgca ggtgctggcg ggcatgcggg
     3241 cgatctgcgc ggcccatggc gtgctgttca tcgccgatga ggtgatgacc ggctggggcc
     3301 gcaccggcac gctgctggct tgcgagcagg ccggcatcac gcccgatatc ctgtgcctgg
     3361 ccaaggggct gacgggcggc tccatcccgt tggcggtgac gatggccagc gccgacatct
     3421 acgccagcca ctattccacc gaccgggcga aaaccttcta ccattcctcc agctacaccg
     3481 ccaatgccat cgcctgtgcg gcggccgcgg ccaatctggg catctggcag gaggaacccg
     3541 tgcgcgaggg catcgccacc cttgccgccc ggcaggcgca aggcgcagcg atgctggccg
     3601 cggtgccggg ggtgggccat gtgcgccagt gcggcaccat cctcgcgctg gattacgagg
     3661 tgggcgcggg cgcgggctat ctctcggccc tgcagccgcg cttgctggcc ttcttccgcg
     3721 aggcgggcct gctgatccgc ccgctgggca acaccgccta tctgatgccg ccctacagca
     3781 tctcggcaga ggatctcgcc cgctgccatg cggcgctggc agaagcggcg gcgctggcaa
     3841 aagggtgaga gggagcggcg ccgcgcgccc cccctgatcg ccctcaatcc tccagcttgt
     3901 agttcttcag ctcgtcgccc ttcagcgtca ccacatggag caggttggtg gcccccggcg
     3961 tgccgaaggg cacgcctgcc agcgtgatca gcttggagcc cgcctcgcca aagccatggc
     4021 gcagggccat gcgcttgccc ttggcgatca tctcctcaaa gctgccgata tccttggtgt
     4081 gcaccgcatg ggcgccccac aaaagggccg ccttgcgcgc ggtcttgagg ctgggggtca
     4141 acaccagcat cggcgccgag ggccgctcgc gcgccacccg ccgggcggtg gagccagagc
     4201 ctgtgaacac cacgatgccg gcgatcgtca gcgtgtcggc aatggccgag ctggccttgg
     4261 cgagggcgtc ggcggtggta tggtcggggt tgatctcggc caggtgcacg cgctggcggt
     4321 aataggtatc gccttccacc tggctggcga tgcggtgcat gatcgacacc gcttcctcgg
     4381 gccactggcc gctggcggtc tcggccgaca gcatcaccac atcggcgcca tcataaacgg
     4441 cattggccac gtccgacact tcggcgcggg tgggggcggg gctctcgatc atcgattcga
     4501 gcatttgggt ggccacgatc accggcttgc cctgccggcg cgtggcttcc acgatgcgct
     4561 tttgcagcgg cggcacttcc tcggggtgca gttccacgcc cagatcgccg cgggccacca
     4621 tgatgccatc ggccagctcg atgatctcgt ccagccggcc gatggcggcg ggcttctcga
     4681 tcttggcgat gatcgccggg ccattggggc cgatcagctt gcgcgcctcg gccacatcct
     4741 cgggccgctg cacgaaggag aggccgaggt aatccacctg ctggtccacc gcgaaggcaa
     4801 ggtcgcgccg gtccttctcg gtcagcgccg gcaccggcac caccgcatcg ggcacgttca
     4861 cccccttgcg atcggaaatc accccgccca cctctgccga gcacaggatc gcatcggcat
     4921 cggcgcggat cacccgcaag cgcagcttgc catcgtcgat cagcaggcgc tggcccttct
     4981 ggagaatgcc gaagagttcc ggatggggca ggcacacgcg ggtttcatcg ccgggcgtgg
     5041 ggtcgcggtc gagcgtgaaa tggctgccat ggcggatcac cttgcgccct tcggtgaagg
     5101 tgcccacgcg cagcttgggc ccctgcagat cggcgaggat ggcgatcggc cggccaaact
     5161 gcttttcggc ggcgcggata tgggcgatgg tctgggcatg ggtggcctga tcgccatggc
     5221 tcatgttgac gcggaaggca tcggcgccgg cgcggatcag cttgccgatc atctcgggct
     5281 cgcggctggc cgggcccagc gtggagagaa tcttcacctt gcgcccgcgg ggattgagct
     5341 tgatcacctt acatcacctc ttttcgcatc cactttgcct atggcaaagc cggggcataa
     5401 acccaaggat caatgccatg aataagaatg tgagcagccc tgccgcagac gcgctggacg
     5461 ggctggacga caaggtggcc gcggccgctt tccgccggct ggtgcgccac ctgcaacacc
     5521 gccacgatgc ccagaatatc gacctgatgg gcctggccgg cttctgccgc aattgcctgg
     5581 cagactggat cgaggaggcc ggcgccggcc ttggcaaggc cggtgcgcgc gaggtgatcc
     5641 atggcctggc cgccgaggaa tggaagcgcc gctatcaatc cccggccacg cccgaacagc
     5701 tggctcgcat ggaagagagc ctgaagaaga acgccgccgg ctgatcatgg ccctttgaac
     5761 acctgccctg cttgggggaa acgggcgggg ccatcttgca ggtatcgccc tggcgcccct
     5821 atgtgccggg ctgaccgatt cacatcttca cgcaaaggat atcttcatgg cagacaatga
     5881 cgcgcaggcc gccgacgaac gcctgcgtct tctcatcgag cgcatcgagc gcctggagga
     5941 ggaaaagaag ggcatcggcg atgatatcaa ggatgtctat ctggaagcga aatcggccgg
     6001 ctatgatccc aaggcgatgc gccaggtggt ccgcatccgc aagatgaagc cggacgaccg
     6061 caaggaaatg gaagcgatcc tcgatgtcta tctgacggcg ctgggcattt cctgagggcg
     6121 ccaggggcgg ggccgcgcgc agccccgccc gcctgccggc accggtcgac gatcggggtg
     6181 ccgcattcaa catgtggcgg gccaatcccc cgccatcagg cttggcaagc ctaactctgc
     6241 tgccaattgc cggcaagacg gcgcgattgc ccttttgcgc cagtttcgct agggggcttg
     6301 ccaaattccc gtcagcaatg aagcgatacc atggcaggcc attccaaatt caagaacatc
     6361 atgcatcgca agggcgcgca ggacaagaag cgctcggcgc agttctccaa gctttcccgc
     6421 gaaattaccg tggcggccaa gatgggcatg cccgatccgg acatgaaccc gcgcctgcgc
     6481 gccgccatca acgcggccaa ggcccagtcc atgccgaagg acaatatcgc ccgcgcgatc
     6541 gacaaggcca gccgcggcga tggcgagaac tatgaagagg tgcgctacga gggctatggc
     6601 ccgggcggcg ttgccatcat cgtggaagcg ctgaccgaca accgcaaccg caccgccacc
     6661 aacgtgcgca ccgcctttgc caagaacggc ggcaatctgg gtgcctcggg cgcggtgtcg
     6721 cacggctttg atcgcctcgg cctcatcgaa tatcccgcca gcgtgggcga tgaggacaag
     6781 gtgctggaag cggcgatcga ggccggcgcc gaggatgtcg aatccgacat gggcgatggc
     6841 gatgaaaacc ccggcagcca ccagatctgg gtggcggtcg aggaactgca cccggtcgcg
     6901 cgcgagctgg aaaaggtgct gggcgaggcc gagggcgtga agctggcctg gcgcccgacg
     6961 ctgaagaccg ccgttgatgc cgacaccgcc gcgacgctgc tgaagctgat cgacgtgctg
     7021 gaagaggatg acgacgtgca gaccgtctgg ggcaactacg acatccccga ggacgtgctg
     7081 gaaaagctgg cctgatgatc agcggcgccc tgatcgccag ggcgctgctg tcgggcgcca
     7141 tcatcgcggc gatcgccgag atcggcaagc gggcgcccac ttttgcggcg ctggtggctt
     7201 cgctgccgct ggtctcggtg ctggggatgg tgttcctgtg gcaggcccgg cccgatgccg
     7261 ccaacatggc cgcccatgcc gaggcaacct tctggtatgt gctgcccagc ctgccgatgt
     7321 tcctgctgat cccggcgctg ctgcgccagg gggtggggtt ctggccggcg ctggggctgg
     7381 gctgcgcgtt gacggtggcg ctgtatctgg gcctggcccg gctggcgccg cgctggggta
     7441 tcttcctgtg agcgttaatc cgtgagcgtt atcgtgatcc tgggcgtgga tccgggcctc
     7501 acctgcaccg gctggggcat tgtggccaag gcgggcagcc gcatcagcca tatcgccaat
     7561 ggccagatcc gcaccgatgt ggccgccccc atggccgagc gcctgctgca cctcgaccgc
     7621 gagctgaccg atgtgatcgc ccgccatcac cccgatacag gggcggtgga ggagatcttc
     7681 gtcaacgtga acccgcagag cacgctgaag ctgggccagg cgcggggcgt ggcgctgctg
     7741 tcgctggcgc gcggcgggct ggatgtggcc gaatatccca ccaagaacat caagaaggcg
     7801 ctggtgggca ccggcaatgc cgaaaagcag caggtgcagc acatgctgaa gatcctgctg
     7861 ccgggcgtga agctggccgg cgccgatgcc gccgatgcgc tggccgtggc gatcacccat
     7921 gcccacatga tgccggcccg cccgctggcc gccggctgaa ccctacatgt cgagccgctg
     7981 cagcaccgcc tgatagcccc agcgcggcgc cggcgtgcag ctgccgggcc gcgtgtagag
     8041 gcacatgctg tccttgccgg aagtgtcgca gcgctggcgg gcatagccct ggggcaccgg
     8101 gccggggctg ggcatcacga tggcattggc cgcgcccagc aggtctgccg gggggcgatc
     8161 ggccacgggg atggtatcga tcggtcgtgt gtcatactgg gagacgatcg gcgggccgag
     8221 atagagcggc agcgggcgcg gcagcatgcc atccaccaca tagtgccgcc acagataggg
     8281 cacggccagg ccgcagatcc ccggctggcg atccgccgcg atcaccatgc cggccagcgc
     8341 gccgcccttg cgcaaggggg caccattttc caccacctgc tgcgcgccga aagaggccgc
     8401 ccaccaggcc ggaaacagcg ccagccacag ccagctgccg gttttggccg ccgtggggct
     8461 ggccgtgccc agccgcccct gcgcccagcg cgcgcccctg accgagccga tcgcccccag
     8521 caccagcaac gacagcagcg acagccagat aaagcggaat tccttgtgcg gaatgaagct
     8581 gtgcgcggcg atattggccc aggccgccac cagcagcggc agatagcggc gccccgcgcc
     8641 gaacatggca gccaggatcg gcagcaccgc cggccccaga tggatgagca gcacgatcac
     8701 aaacgcataa ggcggcgaga ccccgaagat cgaggcgcgt ccatcgccca cattcatcgc
     8761 gtaatagcgc cacagccaca ggaagggcgc ccggcccatc gccagatcgc tgagcgcgcc
     8821 cagcgccagc gccgccagcc cgccctcgat cagccagcgc cagcgccgcc agtctgcctt
     8881 cagcgccatg gccgccagca ccgcggcaaa caccgcatat tgcatgcgga acaccacccc
     8941 cagccccagc agcagcccgg ccgcctgcag cgcgcgccgg ccgggcgcat cggccagcag
     9001 cagggcggcg gccagcagca tcagcgcggc ggccagcgtc tcgctcagca gcagttcgcc
     9061 aaacagcacc gattcgaacc acaccgccac cacgaacagc gccatcagcc cctcgcgccg
     9121 gcccgacagc agccccagcc gccaggccgc cggcaggctg atcagcgcca gcgcggcaaa
     9181 ggcgatgcga gcggccagat agggcgccag cgtgcccggc gccagccagt ggcccagcgc
     9241 caccggccct gccagcagct gcggcagcag ccagctgcgg atgccatagc gatattccca
     9301 gggcaccgtg ccatggccgg tgaccagccg gttggcctgc tcgaaatatt gcatcagctc
     9361 gtcggggtgc agcgtgtcga gcggggagaa ggcccagatc ctcagcgcca cgcccagcag
     9421 catgatcgcg cctgtggccc aaagccagtg gcgggggagc ggcggctggc ccgggcagga
     9481 ggaaaagata cgcatgccaa aggggatagc tgccgggcga gggcctgtcc acgcgggcgc
     9541 ggcggatagt ttgcccccag gttcgcccca gcttcgctct ggccgccggc atgcaaagat
     9601 gttcccaaat cgttccgcgc gcgctagggt gccggcatga ttgcgaaact gaccggcctg
     9661 ctcgacgatt tcggccccga ctgggcgctg atcgatgttg gcggcgtggg ctatctggtg
     9721 cagtgttcgg cgcgcacctt gcatgcgctg ggcctcaggg gcgagcgggt ggtggtgcac
     9781 accgagatgc aggtgtccga gacggacatg cggctgatcg gctttgcctc ggccggagag
     9841 cgggtgtggt tccgcctgtt gaccgcggtg cagggggtgg gcagcaaggt ggcgctggcg
     9901 atcctctcgg cgttgacggt ggaggaattg cagaaggcct gcgcccatgg cgatgcggca
     9961 atggtggcca gggccaatgg cgtgggcccc aagctggccg cgcgcatcgt caacgagctg
    10021 aaggacaagg cgggcgccat ggcttttgcg ggcgggcccg gcgtggtgct ggcaggcggc
    10081 ggcgggggcc ttgcgggcga gggcgggcgg ctggccgatg tcgtctcggc gctgcagaac
    10141 ctgggcttca agccggcagt ggcggcacag gccgcaggcg ccgcgctgga agagctgggc
    10201 gaggaagcgg gcgagggcgc gctgatccgc gccgcgctga agaaggcggc tggctgagat
    10261 gagcaacccc ctcctttccg cccatgccca gatcgaggac atggatgctg cgctgcgccc
    10321 caagaccctg gccgaattcg tgggccagca ggcggcgaag gaaaacctgc gcatcttcat
    10381 cgaaagcgcc aaaagccggc aggaagcgct ggatcacacg ctgttcttcg gcccccccgg
    10441 ccttggcaag accacgctgg cgcagatcat cgcgcgcgag ctgggggtga attttcgcgc
    10501 cacatcgggg ccggtgattg ccaaatcggg cgatctggca gcattgctca ccaatcttga
    10561 gcatggcgat gtgctgttca tcgacgagat tcaccgcctg aacccggtgg tggaggaagt
    10621 gctctatccg gcgatggagg atcgcgcgct ggatctgatg atcggcgagg ggccttcggc
    10681 gcgcagcgtg cggatcgacc tgccgcgctt caccctggtc ggcgc
//
LOCUS       DASRYE010000337         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_68791,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000337 DASRYE010000000
VERSION     DASRYE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68791"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFF98_17495"
     CDS             <1..1110
                     /locus_tag="VFF98_17495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU65486.1"
                     /translation="LLLDQPAAAPATPPAPWWQQAGDGPLAAVVGHALAANPALRCRA
                     AGLAASQRHASQSLGARLARAFGARRLDTPRTPPPADEEAYATARMATALSVGLAYVD
                     LRLAQERLALREAAIAPLKDNREIARFRREAGLVSAVDADLAGTMVDLNEDALAGARA
                     ARDAALARLAALAGMSSADLAAAMGPVGPSSHVPGWQAAPAPRPFTPAPDARADIAAL
                     ALRLGAGLAAAHVAPADIAAARAPAAHGKPSARQRQAAAALAQLRQAEGAATAELQAA
                     DAALLGARQRAAVLAGRLPGTARALADVRLGYRSGTETFATLYVAEGAALALAEAEAE
                     ARAGAARAALGWWQAQALGWQSPAPLATAEGAACD"
     gene            1103..2275
                     /locus_tag="VFF98_17500"
     CDS             1103..2275
                     /locus_tag="VFF98_17500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZU65487.1"
                     /translation="MTDGDLDTFLGSGSAPRRRPWATLAVLAAVLVAAFLLLMRFFNG
                     PDLPYYSAPVERADLTPVLAARGSVHGENEVILRAPMDGTIASLQGPAEGPVAAGQVL
                     ATLDSDALRSGLAAAQMRVAETTATLASAQAGAEEAGSRMERFDTVWRKSAGRVPSLG
                     EMDHARADLQRAIAALVAARADLATARQEAANRQADLDAASLKAPFAGYLAARHVDQG
                     QPVVAGAPVVTLLPDARRLEASVVLGTAEAMRLPAGAHARVLVAGTDKPVEATLLRLE
                     AGRDDAHARALFRLSAPQPGLRVGTPAAAEIDLPLRRQVLVVPDAALGFAPLGAPANR
                     GDAVYVLDDSGQTRRVQVIRANTDGQRSEVLSAALQPGMQVVTGWRHAPPAAAKAP"
     gene            2304..3806
                     /locus_tag="VFF98_17505"
     CDS             2304..3806
                     /locus_tag="VFF98_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HZU65488.1"
                     /translation="MPPAPSSHRPTRRDLARWLGALVAGACLPAGLKAAPAGRFAPAR
                     AFSPAALVAQARAAARRPYAARPLSAHAAVDFDAHVRLAYGAAEEMPGHIRLFPTRRD
                     IGAGSVDIHLVEGGMARQLIDTHGLFGSGEAADAAGFRAMYADGHADWLAFLGASYFR
                     AVGGSEQYGLSARGVAVNTSLPGREEFPAFTAFWLEPLGEQRFNVHALLDGPSLTGAF
                     SIATAREQGRTVQDVTASLFFRQDIAQLGLAPMTSMFFLGESTTDHTRDWRPEVHDSD
                     GLAIHMAGGERIWRPLDNPPEPRTHSFRADAPRGFGLLQRDRAFDHYLDDQSFFDRRP
                     SLWVEPKGHWGKGQVVLYAFPTASENVDNVAAFWQADAPARAGGRRDLAYRLTWSGWA
                     PADANACCVDVHQGPGGVPGAEPIAGATRYVFDFAGPVLAGISSASAITDLPKEAVLL
                     SHVAPVAGQPARWRVTLDVRTQGLAQADFRLFLAHGPQALSETVIQPVRA"
     gene            3806..5554
                     /gene="mdoH"
                     /locus_tag="VFF98_17510"
     CDS             3806..5554
                     /gene="mdoH"
                     /locus_tag="VFF98_17510"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HZU65489.1"
                     /translation="MRQPAPLPPEAPLDMPVQGLDGIPPGARAVDTRPQGLLVKRLLL
                     IGLTAVISLAASSELRASLSGDGLGAFDVALLLFFVPLFSWLAFGFVSSAIGFWRLIS
                     GGHPGFVQIPAPRAPLARRTAVLMPIYNESVEATFGRVRAMTKAIADAGGAGQIDFFV
                     LSDSGAHNGPAEEAAWRAVAATAPIPVYYRRREQNIARKPGNIAEWVRRFGGAYECML
                     VLDADSIMSGQAIVGMAAVMEARSSIGLLQTVPKIVNAHTFYQRWMRFAAEAYGPIAS
                     AGLLWWAGSEGNFWGHNAILRTRAFAESCGLPELPGKPPFGGHIQSHDMVEAALLRRR
                     GWAVHMVMITGSYEEFPPNLVDNAIRDRRWMQGNLQHLRLLGAAGLSATSRLHFFIGA
                     TAYLTSPGWLLLLLTTIAQAATGKPAMPLAIGPYVLALTVVLLFGPKAMGLVWMLASA
                     QRRRDFGGVGAILRSVLADVVLAMLQAPVTMVMQTKALVALVLGIPSGWHTQNREAQR
                     IPLRLILPDLREQLALGLLFAATGLFSPITAVWLIPLTAGLLLSPWLISLSSSEELGA
                     RAARLNLFTAPPPDFD"
     gene            complement(5560..>6001)
                     /locus_tag="VFF98_17515"
     CDS             complement(5560..>6001)
                     /locus_tag="VFF98_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZU65490.1"
                     /translation="SGGSAKNALPERAIANINCRILPGETRDGVAARLAAAIADPAVA
                     ISMPDGAGPTAVPVPLDEAVMAPIRQVAARYYPGVPVIPTLVSGSTDGAFLGAAGIPT
                     YGVPSFFYDADFGHIHGVNERIRVDSVLTGRDMLYDLVVALAGG"
BASE COUNT          765 a         2247 c         2079 g          910 t
ORIGIN      
        1 ctgctgctgg accagccggc cgccgcccct gccacgcccc ccgcgccctg gtggcagcag
       61 gccggcgatg gcccgctggc cgccgtagtg ggccatgcgc tggcggccaa tccggccctg
      121 cgctgccgcg ccgccgggct ggccgccagc cagcgccatg ccagccagag cctgggcgca
      181 cgcctggccc gggctttcgg ggcccgccgc cttgacacgc cccgcacccc gcccccggcc
      241 gacgaggaag cctatgccac cgcgcgcatg gcaacggcgc tgtccgtggg cctggcctat
      301 gtcgatctgc gcctcgcgca ggagcggctg gccctgcgcg aggcggccat cgcccccctg
      361 aaggacaacc gcgagatcgc ccgcttccgc cgcgaggccg ggctggtgtc tgccgtggat
      421 gccgatctgg ccggcacgat ggtggatctt aacgaggatg cgctggccgg tgcccgcgcc
      481 gcccgcgatg cagcgcttgc ccggctggct gcgctggccg gcatgagcag cgccgatctc
      541 gccgcggcga tgggccctgt tggcccttcc agccacgtgc ccggctggca ggcggccccg
      601 gcgccccggc ccttcacccc ggcgcccgat gcccgcgccg atatcgccgc cctggccctg
      661 cggctgggcg cggggctggc cgcagcgcat gtggcgccgg ccgatattgc cgccgcccgc
      721 gcgcctgccg ctcatggcaa gccatcggcc cggcagcgcc aggccgccgc cgcgctggcc
      781 caactgcgcc aggccgaagg tgccgccacc gccgagctgc aagccgccga tgccgccttg
      841 ctgggcgccc gccagcgggc cgccgtgctg gccggccgcc tgcccggcac ggcccgcgcg
      901 ctggccgatg tgcggctggg ctatcgcagc ggcaccgaaa ctttcgccac gctctatgtc
      961 gccgagggcg ccgcccttgc cctggccgag gccgaggccg aggcccgcgc cggcgccgcc
     1021 cgggcagcac tgggctggtg gcaggcccag gcgctgggct ggcaaagccc cgccccgctg
     1081 gcaaccgcag agggggcggc ctgtgactga cggcgatctc gatacctttc tgggcagcgg
     1141 cagcgcgccc cgccggcgcc cctgggccac gctggcggtg ctggcggcgg tgctggtggc
     1201 cgccttcctg ctgctgatgc gcttcttcaa cgggcctgat ctgccctact acagcgcgcc
     1261 ggtcgaacga gcggatctga ccccggtgct ggccgcccgt ggcagcgtgc atggcgagaa
     1321 cgaggtgatc ctgcgcgcgc cgatggatgg caccatcgcc agcctgcaag ggccggccga
     1381 agggccagtg gccgccgggc aagtgctggc cacgctggat agcgatgcgc tgcgatcggg
     1441 ccttgccgcc gcgcagatgc gcgtggccga gaccaccgcc accctcgcca gcgcccaggc
     1501 cggcgccgag gaagccggat cgcggatgga gcggttcgac acggtgtggc gcaaatcggc
     1561 cgggcgggtg ccctcgctgg gcgagatgga ccatgcccgc gccgacctgc aacgcgccat
     1621 cgccgcgctg gtggccgccc gcgccgatct ggccaccgcc cggcaggaag cggccaaccg
     1681 ccaggccgat cttgatgccg ccagcctgaa ggcgcccttt gccggctatc tggccgcgcg
     1741 ccatgtcgat cagggccagc cggtggtggc cggcgccccg gtggtcacgc tgctgcccga
     1801 tgcccgccgg ctggaggcca gcgtggtgct gggcacggcc gaggccatgc gcctgcccgc
     1861 cggcgcccat gcccgcgtgc tggtggccgg caccgacaag ccggtggagg ccaccctgct
     1921 gcggctggag gccgggcgcg atgatgccca tgcccgcgcc ctgttccgcc tgtcagcgcc
     1981 gcagccaggc ctgcgcgtgg gcacgccggc cgccgccgag atcgacctgc ccctgcgccg
     2041 gcaggtgctg gtggtgcccg atgcggcgct gggctttgcc ccgctggggg cgcccgccaa
     2101 ccgcggcgat gccgtctatg tgctggacga cagcggccag acacgccgcg ttcaggtcat
     2161 ccgcgccaac accgacgggc agcgcagcga agtgctttcg gccgccctgc aaccgggcat
     2221 gcaggtggtg acaggctggc gccatgcccc tcccgccgcc gccaaggccc cctgaccctg
     2281 ccgccaccaa ggaacgcgcg cccatgcccc ctgccccctc ctcccatcgc cccacccgcc
     2341 gcgatctggc ccgctggctg ggggcgctgg tggcgggcgc ctgcctgccg gcggggctga
     2401 aggcagcgcc tgcgggccgc tttgccccgg cgcgcgcctt ctcgccggca gcgctggtgg
     2461 cgcaggcccg ggcagcggcg cgccggccct atgccgctcg cccgctatca gcccatgcgg
     2521 cagtggattt cgatgcccat gtgcgcctgg cctatggcgc ggccgaggaa atgcccggcc
     2581 acatccgcct gttccccacc cggcgcgaca ttggcgcggg cagcgtcgac atccaccttg
     2641 tggaaggcgg catggcccgc cagctgatcg acacgcacgg cctgttcggc agcggcgagg
     2701 cggccgatgc cgccggtttc cgcgccatgt atgccgatgg ccatgccgac tggctggcgt
     2761 tcctcggcgc ttcctatttc cgcgcggtgg gcggcagcga gcaatatggc ctttccgccc
     2821 gcggcgtggc ggtgaacacc agcctgcccg gccgcgagga atttcccgcc ttcaccgcct
     2881 tctggctgga gcctttgggc gagcagcgct tcaatgtgca tgccctgctt gatggcccct
     2941 cgctcaccgg tgccttctcc atcgccaccg cgcgcgaaca ggggcgcacc gtgcaggatg
     3001 tcaccgccag cctgttcttc cggcaagaca tcgcccagct gggcctggcg ccgatgacca
     3061 gcatgttctt cctgggcgaa agcaccaccg accacacccg cgactggcgc cccgaagtgc
     3121 atgacagcga tgggctggcc atccacatgg ccggcggcga gcgcatctgg cgcccgctcg
     3181 acaatccgcc ggagccgcgc acccacagct ttcgcgccga tgccccgcgc ggcttcggcc
     3241 tcctccagcg cgatcgtgcc ttcgaccatt atctcgacga ccagagcttc ttcgaccgcc
     3301 gcccctcgct gtgggtggaa cccaagggcc attggggcaa ggggcaggtg gtgctctatg
     3361 ccttccccac cgcttccgag aatgtcgata atgtcgccgc cttctggcag gccgatgccc
     3421 cggcccgcgc cggcggccgg cgcgatctgg cctatcggct gacctggagc ggctgggcgc
     3481 cggccgatgc caatgcctgc tgcgtggatg tgcaccaggg ccccggcggc gtgcccggcg
     3541 ccgagccgat cgccggcgcc acgcgctatg tgttcgattt tgcagggccg gtgctggccg
     3601 ggatcagcag cgccagcgcc atcaccgatc tgccgaagga ggccgtgctg ctctctcatg
     3661 tcgcaccggt ggccgggcag ccggcgcgct ggcgggtaac gctggatgtg cgcacgcaag
     3721 ggttggccca ggccgatttc cgcctgttcc tcgcccatgg cccgcaagcg ctgagcgaaa
     3781 ccgtgatcca gccggtgcgc gcctgatgcg ccagcctgcc ccgctgcccc ctgaagcccc
     3841 gctcgacatg ccggtgcagg ggctggatgg catccccccc ggcgcccgcg cggtggatac
     3901 ccgcccgcaa ggcctgctgg tgaagcgcct gctgctgatc ggccttaccg ccgtcatcag
     3961 cctggccgcc tccagcgagc tgcgcgccag cctctcgggc gatgggctgg gcgccttcga
     4021 tgtggcgctg ctgctgttct tcgtgccgct gttcagctgg ctggcctttg gctttgtctc
     4081 ctcggcgatc ggcttctggc ggctgatctc gggcgggcat ccgggctttg tgcagattcc
     4141 ggcgccgcgc gcgcccctgg cccggcgcac cgcggtgctg atgccaatct acaacgaaag
     4201 cgtggaggcc actttcggcc gcgtgcgggc gatgaccaaa gcgatcgccg atgcaggcgg
     4261 ggccggccag atcgatttct tcgtcctcag cgattccggt gcgcacaacg gccctgccga
     4321 ggaagccgcc tggcgcgcag tggccgccac agcgccgatc cctgtctatt accgccggcg
     4381 cgaacagaac atcgcccgca agcccggcaa catcgccgaa tgggtgcgcc gctttggcgg
     4441 agcctatgaa tgcatgctgg tgctcgatgc cgacagcatc atgagcgggc aggcgatcgt
     4501 gggcatggcc gcggtgatgg aagcgcgcag ttccatcggc ctgttgcaaa ccgtgcccaa
     4561 gatcgtcaac gcccacacct tctaccagcg ctggatgcgc tttgccgccg aggcctatgg
     4621 gccgatcgcg tcggccgggc tgctgtggtg ggcgggctcg gaaggcaatt tctggggcca
     4681 caacgccatc ttgcgcaccc gcgcctttgc cgaaagctgc ggcctgccgg aattgcccgg
     4741 caagcccccc ttcggcggcc atatccagag ccacgacatg gtggaagcgg ccctgctgcg
     4801 ccggcgcggc tgggcggtgc acatggtgat gatcaccggc agctacgagg aatttccgcc
     4861 caatctggtg gacaacgcca tccgcgaccg gcgctggatg cagggcaacc tgcagcattt
     4921 gcggctgctg ggcgcggccg gcctcagcgc caccagccgg cttcacttct tcatcggcgc
     4981 cacggcctat ctcacttcgc ccggctggct gctgctgctg ctcaccacca ttgcccaggc
     5041 ggccaccggc aagcccgcca tgccgctggc gatcggcccc tatgtgctgg cgttaaccgt
     5101 ggtgctgttg ttcggcccca aggcgatggg gctggtgtgg atgctggcct cggcccagcg
     5161 gcggcgcgat tttggcgggg tgggcgcgat tttgcgctcg gtgctggccg atgtggtgct
     5221 ggccatgctg caggcgccgg taacgatggt gatgcagacc aaggcgctgg ttgccctggt
     5281 gctgggcatc ccatctggct ggcacacgca gaaccgcgag gcccagcgca tcccccttcg
     5341 cctcatcctg ccagacctgc gcgagcaact ggcgctgggc ctgctgtttg ccgccaccgg
     5401 cctgttctcg ccgatcaccg ctgtgtggct gatcccgctg acggcggggc tgctgctctc
     5461 gccctggctg atcagcctgt cgtccagcga ggaactgggg gcaagggcgg cccgcctgaa
     5521 cctgttcacc gcgccgcccc ccgatttcga ctgagcgctt caaccgccag ccagcgccac
     5581 caccagatcg tagagcatgt cgcgcccggt cagcacgctg tccacccgga tgcgctcgtt
     5641 cacgccatgg atatggccga aatcggcatc gtagaagaag ctgggcacgc cataggtggg
     5701 aatgccggca gcgcccagaa aagccccgtc ggtggagccg gagaccagcg tggggatcac
     5761 cggcacgccc ggataatagc gcgccgccac ctgccggatc ggcgccatca cggcctcgtc
     5821 cagcggcacc ggcaccgccg tggggccggc gccatcgggc atggagatcg ccaccgccgg
     5881 atcggcaatc gccgctgcca ggcgggctgc caccccatcg cgggtttcgc ccggcaggat
     5941 gcggcagttg atattggcga tggcgcgttc gggcagggcg ttcttggcgc tgccgccgct
     6001 g
//
LOCUS       DASRYE010000338        22137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_51022,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000338 DASRYE010000000
VERSION     DASRYE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 22137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22137
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51022"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFF98_17520"
     CDS             complement(<1..278)
                     /locus_tag="VFF98_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HZU65491.1"
                     /translation="MIVFDLECQAGGHRFEGWFGSSADFASQQAAGLVVCPQCGSADV
                     LKAPMAPHVGRKGNQMPALAPTEPSGPAASSAPAAAATPPAAPAARPDP"
     gene            complement(275..1102)
                     /locus_tag="VFF98_17525"
     CDS             complement(275..1102)
                     /locus_tag="VFF98_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZU65492.1"
                     /translation="MSRIALLQMTSGIDPAANAATIAEGLMRAGEGGAQMLFTPEMAC
                     LIDRDRARARAHVVPESASPVLDALRAAAAKSGVWLALGSLAVDREDGRWANRSFVID
                     STGEIRARYDKIHMFDVDLETGESWRESAAYAPGEAVVVAETPVGRLGLAICYDLRFP
                     ALFEALGQARCDLIAIPAAFTVPTGRAHWHLLQRARAVEASAYVVSAAQVGQHADGRA
                     TYGHSLVVDPWGEVLLDMGGEAAGLAFAEITPERIAKVRRELPALANRREIPRSVLS"
     gene            complement(1109..1369)
                     /gene="grxC"
                     /locus_tag="VFF98_17530"
     CDS             complement(1109..1369)
                     /gene="grxC"
                     /locus_tag="VFF98_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZU65493.1"
                     /translation="MSAKVEIYTQWGCPYCTRAKALLDAKGVAYEEIDVTMDTRKRAE
                     MAERKPGARTVPQIFADGVALGGCDDIHALDAAGKLDAALGL"
     gene            1576..2058
                     /locus_tag="VFF98_17535"
     CDS             1576..2058
                     /locus_tag="VFF98_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HZU65494.1"
                     /translation="MNRFDFTPYRRNTVGFDRLFDLLENATRAGAGDNYPPFNIERRG
                     EDTYRITLAVAGFKPGEIDITAQQNLLVIQGAKAEDPAAAGHYLHVGIAQRGFERRFE
                     LADFVRVDSATLEDGLLVIDLVREVPEAMKPKKIAIGPATTLSVVGGVAGNGPDSAAA
                     "
     gene            complement(2376..4007)
                     /locus_tag="VFF98_17540"
     CDS             complement(2376..4007)
                     /locus_tag="VFF98_17540"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZU65495.1"
                     /translation="MARFIFITGGVVSSLGKGLMAASLAALLQARGFKVRIRKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVSARKSDNITSGRIYQTIIAKE
                     RRGDYLGATVQVIPHVTDAIKEFAQAETDDLDFVLCEIGGTVGDIEGLPFIEAIRQLR
                     NELGREQTCFAHVTLVPYIKAAGELKTKPTQHSVRELTSLGIQPDVLLCRCEHPLPEN
                     ERNKIALFCNVRKEAVIPALDAPNIYAVPVQYHAEGLDAAVLHHFGLAAPEPALARWE
                     NLTQLYANPEGEVTIGVVGKYVVLQDAYKSLNEALVHGGLANKVKVHIKWIDAEIFEK
                     DDGEIAATLEPLHGILVPGGFGERGTEGKIAAVRFARERKVPFLGICLGMQMACIEGA
                     RNTAGIADASSTEFGETTEPVVGIITEWMSEEGLQTRSTDTDLGGTMRLGAYEAKLDG
                     NSHVASIYGSETIFERHRHRYEVNSHYREALEKGGLVFSGMSPDGLLPEIVERPDHPW
                     FVGVQFHPELKSRPFEPHPLFAGFIGAALRQARLV"
     gene            complement(4262..4627)
                     /gene="secG"
                     /locus_tag="VFF98_17545"
     CDS             complement(4262..4627)
                     /gene="secG"
                     /locus_tag="VFF98_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZU65496.1"
                     /translation="MFIFLTVLQALIGAAMVAVILVQKSEGGGLGTGGSPTGFMSARG
                     AADFLTRTTQVLAGLFVLLSIVLAALAVKSGGGETVDTSLVRNVPAAVPSAAPTVAPS
                     APASTAPAAADPLAPAAKQ"
     gene            complement(4807..5565)
                     /gene="tpiA"
                     /locus_tag="VFF98_17550"
     CDS             complement(4807..5565)
                     /gene="tpiA"
                     /locus_tag="VFF98_17550"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZU65497.1"
                     /translation="MALRPYIVGNWKMNGVRTSLPEARAIDRAATRAPQVDVALAPPF
                     TLIHSMAEAVQAIAVGGQDCHARANGAFTGDVSAPMLADAGASFVILGHSERRSLHGE
                     SDSDVRAKVDAALAADLGIIVCVGETEKQRDAGQAEKVVTGQVEGSLPADAAALARLS
                     VAYEPVWAIGTGRVPSVADVGAMHRAIRARLVALYGDAGAKVRILYGGSVNAANAGEL
                     LAADEVGGALVGGASLTAESFAAIVVAAAMLTES"
     gene            5932..7884
                     /locus_tag="VFF98_17555"
     CDS             5932..7884
                     /locus_tag="VFF98_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZU65498.1"
                     /translation="MLQFFRNFFKSTLGVGITLGLLVLIALAFAAGDIAGNGALSGLG
                     GAKVATVGREAITPAQLTKQLTQAAELARQDNPRLTMKEFLAEGGLEDVLSSMIDRTA
                     MVVFGKAQGVVAGKRLVDSELARLPQLQNPDGSFSQSAYRQLLAQRQLTDADVRADIG
                     QGLVARQLLAPAELAIGMPASIAQRYAGLLTERREGTVAVLPAALFAPKDKPADADVA
                     RFYAAHLATYNRPERRVIRYALFDDSVAKQVAAPTEAEIAQRYGANKAQYAASESRHI
                     VQLILPGEEAAKALAAELAKGQSLEAAARARGLATAPLGPLSHDALAQQTSGAIADAV
                     FAAKKGALVGPVKGALGYALIRVDEVVTKPARTLDQAHGEIAAQLASEKRKAAIGALT
                     ARIDDNLGHGGALSDAAKDLGLTLSATAPLTADGQVYPGAAGAPLPTALSAKEQASIV
                     KAAFAMEHEGQPQLAELEAGKRYVIYDVSQITAAAPAPLADIRQQVAADYALTKGSAA
                     AQAAAMNVLKAVKGGKDLAATLGGLGVTLPPAQPVAMSRQQLGAFQGRVPPAIQLLFS
                     MAEGTVKLLPAPGGQGWFVVQLKHIQLGTPAANDPAVAQVRQQLGQLVARESGEGLRL
                     AIRAEAGTTRNEAAIRAVVGQLVGGN"
     gene            7931..9469
                     /gene="trpE"
                     /locus_tag="VFF98_17560"
     CDS             7931..9469
                     /gene="trpE"
                     /locus_tag="VFF98_17560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZU65499.1"
                     /translation="MSLSADTAPPHAENTAAARAALAAGKGALVWRRLIADTETPVGT
                     ALKLMRADRGDFLLESVEGGEVRGRYSLLGLDPDLVFRARGHAAEINRSWPHAPEAFA
                     PLAGDPLAELRALVAECRVDVPAALPPALACLVGYFGYETFGLVEKLPQPPANPLAVP
                     DMLFVRPRLLLVFDRLGDELFAVAPVWPSATAPDDAITTAAERIDEAARQLALPVPAQ
                     PRLAADTPLPTLTPTMAPGRYGEMVERARDYIQAGDIFQVVLAQRFTAPFPLPPMALY
                     RALRRVNPSPFLYFLDLPGFALIGSSPEILVRVRDGEVTIRPIAGTRPRGKTPEEDRA
                     NEESLLADPKERAEHLMLLDLGRNDVGRVAAAGTVKVTDSYTIERYSHVMHIVSNVVG
                     RLDTDRADAIDAVFAGFPAGTVSGAPKVRACQIIAELEGETRGAYAGGVGYFGPDGNL
                     DSCIVLRTAVLKDGVMHVQAGAGIVADSDPAYEQRECEHKAGALFAAAREAIRVASEA
                     QFGQ"
     gene            9484..10086
                     /locus_tag="VFF98_17565"
     CDS             9484..10086
                     /locus_tag="VFF98_17565"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZU65500.1"
                     /translation="MILVIDNYDSFTWNLVHYVMELGARVEVVRNDALTAAQALETGA
                     AGFLLSPGPCTPNEAGISLDLVGAAADAGRPLLGVCLGHQSIGQYFGGKVVRGGLMHG
                     KTSPVTHDGTGLFAGLPSPFTATRYHSLIVTDVPDCLVVNARSDDGHTMGFRHASLPI
                     HGVQFHPESIATEHGHAMLANFLKICGIATSLPAHPVLHA"
     gene            10083..11093
                     /gene="trpD"
                     /locus_tag="VFF98_17570"
     CDS             10083..11093
                     /gene="trpD"
                     /locus_tag="VFF98_17570"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZU65501.1"
                     /translation="MSSLPPVGAQPFGEEEAEALFGAILDAQVEAEAIAAFLVALSDR
                     GETASEITGAARAMRARMVPVAAPADVLARAIDVCGTGGDGHHTLNVSTAVSLVVAAC
                     GVPVAKHGNRAASSKSGAADTLEALGLDLARAGERAGETLAEIGIAFLFAQAHHPRLG
                     PIAPIRRALGRRTIFNLMGPLANPAGVRRQLVGIARPAYVPIYADAICRLGTERSFVV
                     SGDEGLDELSLAGGNEWADVQRDTVAMKRVAAAEAGLAASPVSAIRGGDAAHNADALK
                     RLLMGEEGAYRDAVLFNAAGALMVAGEVADWREGVEEAAEAIDKGLAKALLDCWISAT
                     RA"
     gene            11093..11881
                     /gene="trpC"
                     /locus_tag="VFF98_17575"
     CDS             11093..11881
                     /gene="trpC"
                     /locus_tag="VFF98_17575"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZU65502.1"
                     /translation="MTDKLTEICDTKRGEVAARKALTTLAELDARAATQSAPRGFEAA
                     LSAKAATGFALIAEIKKASPSKGLIRADFDPPAHARAYAEGGAACLSVLTDAPYFQGH
                     EDFLVAARGACALPVIRKDFMVDPWQVAEARSIGADAILIIVAALDDGQMAEIEAAAR
                     HYGMDALVEVHNEAEMERAARLTSRLIGVNNRDLKRFVTDLGTTERLAPLAPEGTLLV
                     AESGINSHADLLRLEACGARTFLVGESLMRHADVAAATRALLHG"
     gene            11874..12362
                     /gene="moaC"
                     /locus_tag="VFF98_17580"
     CDS             11874..12362
                     /gene="moaC"
                     /locus_tag="VFF98_17580"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZU65503.1"
                     /translation="MAELTHLDSEGQARMVDVGGKAESRRVAIAEGRIRMNAAALEAI
                     RAGAVPKGDVLAAARIAGIMAAKKTAELIPLCHPLALSSVEVACTLEADGVRVAATAS
                     LTGRTGVEMEALSAASIALLTIYDMAKAIDKAMVIDSVRLIEKRGGKSGDWFAPGHQP
                     AP"
     gene            12359..13564
                     /gene="glp"
                     /locus_tag="VFF98_17585"
     CDS             12359..13564
                     /gene="glp"
                     /locus_tag="VFF98_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZU65504.1"
                     /translation="MSPPLLPLDEAQARLLALATPLPVEHADMASALGHYLAEPLHAH
                     RTQPAADLSAMDGYAVAPGDLAGPWQVVGESAAGRPFGGHLQAGEAARVSTGAVLPAG
                     AGAVLLQEDITRSGAHIALTGTAPAPHDRHIRRAGFDFAEGQLLLPAGTRMGAAQVAL
                     AISGGHGHVAVRRPVRLAILDSGDELAAPGQPCQPHQIPASNGAMLAALVAALPCEIT
                     RAGPVADRLELLVAALDGLGAADVIVTSGGASVGDHDLIRPALEAWGAELDFWKVAMR
                     PGKPLLVARRGRQVILGLPGNPVSALATAYFFLRPLLRKLLGASQCLPRPICTTLAAP
                     LGAGSGRRDFPRGVWDGAMVRPLGGRDSSALAELAGANCLIDRPIGQPALETGKKVNI
                     YLIEPQGFA"
     gene            13643..14353
                     /gene="lexA"
                     /locus_tag="VFF98_17590"
     CDS             13643..14353
                     /gene="lexA"
                     /locus_tag="VFF98_17590"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZU65505.1"
                     /translation="MLTRKQHELLRFIQDRLEETGVSPSFEEMKEALDLKSKSGVHRL
                     ISALEERGFIRRLPNRARALEVLRQPEDAVAPSPRAAARTLATPAPAIGRPLAPMPQV
                     AAFTPANDIVEIPLHGRIAAGTPIEALEGQSMLPVPAALLGSGEHYALEVSGDSMVEA
                     GILDGDLALIRRTEVARDGEIVVALVRGEEATLKFLRHEGGMVRLDPANAAYEPQFYR
                     PAEVRVQGKLAGLLRRYH"
     gene            complement(14347..16488)
                     /locus_tag="VFF98_17595"
     CDS             complement(14347..16488)
                     /locus_tag="VFF98_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZU65506.1"
                     /translation="MARAAEVVEQFLEGAGFDRAPWAVVAFGAGVAFWFVLANQAEWL
                     GLICTGLAAAFLAWALWPKNGRFPQLRQAVIGVGLMLAAGCACVWGKAVLVGAPPIAH
                     QIAGVMVGTVLDRYDQPALARARLEIATREPRTGRLIRVRLTLPLAADAPGVVEGAVI
                     AFHARLMPPSPMRFPGTYDFAEAAWFDGVSATGSVLGKVVLARPALAPDWLARTQHAL
                     AEHVRAQLAGSPGAIAAAFASGDRGGITPQDDQAMRDAGLTHLLSVSGLHVSAVVGGV
                     YVLTLRLLALWPWAALRWRLPLVASSMGGIAGIVYTLLTGAQVPTVRSCLGALLVLAA
                     VALGRQPLSVRMLAVAALVVMVLWPDAVVGPSFQMSFGSVLAIIAVADSAPARAFLAP
                     REEGWWWRIGRHLFMILLTGMVIDLALMPVALYHFHRAGIYGSMANVLAIPLTTFISM
                     PMIGLALALDLAGLGAPAWWVCGKSLEALLAIAHFTAARPDAVSVGPAMGGGAFALFV
                     GAMLWLALWHGRVRLLGLAPALLAVGWLALVRPPDVLIAGDGTNLAISEPDNGRLLLL
                     RPGKSDFTRATMLEAAGISGDVAPLESWPGARCNPDACLVQLSRGGRIWRLLVLRKEA
                     MRMAPAALARACAAVDIVVARGKIFGPCAPGLIRADHVLLLRTGGLALDLVNRRVASV
                     GESEGDHPWWRAPHIAPRDQRPGADTADPAQ"
     gene            16635..18101
                     /gene="gltX"
                     /locus_tag="VFF98_17600"
     CDS             16635..18101
                     /gene="gltX"
                     /locus_tag="VFF98_17600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZU65507.1"
                     /translation="MASASVEGDTGHKGRRVVTRFAPSPTGYLHIGGARTALFNWLFA
                     RHHGGTYLLRIEDTDRQRSTDAAIDAIFDGLRWLGLDGDEPAVFQFERSARHAEVAHQ
                     LLAAGHAYRCYLTQEELAAMRETAQAEKRPFRIQSPWRDAAEGSQPEGKPYVIRIKAP
                     QSGETVITDLVQGAITVQNTELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDH
                     INNAFRQLVILRAMDAIEGGWPDPAYGHIPLIHGADGAKLSKRHGALGVDAYRDDMGL
                     LPEAVINYLMRLGWGHGDEEIISRAQAIEWFDIVNVNKGASRFDFAKLANLNGHYIRE
                     ADDARLADLVAPRIAGDIDLGLLARAMPVLKPRAKDLNELADGAGFLFAQRPLAISEK
                     AAALLTEDARQLLSDLHALLRKFPETEWALERLEEALKAYAAERNLGLGKLAQPLRAA
                     LTGQTTSPGIFDVLVLLGQDEALARIGAQATLATPGAV"
     gene            18113..19396
                     /gene="gltA"
                     /locus_tag="VFF98_17605"
     CDS             18113..19396
                     /gene="gltA"
                     /locus_tag="VFF98_17605"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZU65508.1"
                     /translation="MADVAKLSVGGKELEAPVLKGTVGPDVIDIRKLYGQTGAFTFDP
                     GFTSTASCESALTYIDGDEGVLLHRGYPIGQLAEHSSFLEVCYLLLNGELPNQAELGK
                     FTSTITRHTMVHEQLTKFFSGFRRDAHPMAVMCGVVGALSAFYHDSTDIADPEQRRIA
                     SHRLIAKIPTIAAMAYKYSVGQPFVYPDNKLSYAGNFLKMTFGVPAEEYEVKPAIEKA
                     MDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLR
                     EIGTPDKIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQKTLREVFDALGVNDP
                     LFETALRLEELALNDPYFIEKKLFPNVDFYSGIILSAIGFPTTMFTVLFALARTVGWV
                     AQWNEMISDPNQKIGRPRQLYTGPVQRDYVPLGQR"
     gene            complement(19502..20884)
                     /locus_tag="VFF98_17610"
     CDS             complement(19502..20884)
                     /locus_tag="VFF98_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZU65509.1"
                     /translation="MGPLGQGPMARAASDQNDRLIAADEPLAGLQQRCGGTMPGAIAV
                     PELRELVAKARRFGLKLARPILAQDGDETIRAWVEVIPEADRAEGGCAILLHSWQATP
                     VPAEEPELAARRRAEIDRALAELTARLDEHQGLLTVEGEAGDLLALSTAMRRGIGRPW
                     TDFVTIDGNIHRQPLHWQLLDGARVEVSGSQRRWRATLIPIAGPANQPAGFDLCLTAD
                     RPLPAPPRKAGQPGSNLPETALVGQDLAPVLRQPIARIIANAETIRTRLAGPLAEEYA
                     QYAADIAAAGQHLQDLIDDLTDLEVIEQPHFTTAPDRIDLADVARRAAGILGMRARQK
                     GIAILLPEGEVRLPAVAEFRRALQILLNLIGNAIRYAPEGSRIHLEYGQAGALVRLVV
                     ADEGPGLSAEEQARVFNKFERLGRSGDGGSGLGLFISRRLARAMGGELAVESRKGEGA
                     RFLLDLPAQG"
     gene            complement(20884..21828)
                     /locus_tag="VFF98_17615"
     CDS             complement(20884..21828)
                     /locus_tag="VFF98_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65510.1"
                     /translation="MLSSYDGRMNEGSVQSIAADPAEAALCGELARGDAVLQSVDPVL
                     RHLLMQRGGALFADDVLARVKGMLADMACQLLDAGQLLDASGGEAAVSALAGRLADVP
                     GLLGHLHAQALEWQVAQRLQAQLGLDPVLSPLLQALVGSTQPDVAALAMRLLAAQARF
                     ARQISRMQWPLAELPGDLLHAVLAAWEAEGGATAAVREAYEEGHTRHALAARLVTMMG
                     AGALAALLPGHAGVALFASAVAQGAQGARDLAVLAMQEGQKTRLALMLRAGGAPPATV
                     EDALLALFGGSAEDAELARVGAEKAARLLTSGAATGAV"
     gene            complement(21828..>22137)
                     /locus_tag="VFF98_17620"
     CDS             complement(21828..>22137)
                     /locus_tag="VFF98_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65511.1"
                     /translation="GRWVLAVGDGALVLVLAWSGAGGDPLWGRVYPPLVLIGLVRLLA
                     WLAPGGLGGWAADRACLAALLALAALAGVLGGAVKLLGLALLVGALACEGREKRLTAL
                     "
BASE COUNT         3589 a         7828 c         7411 g         3309 t
ORIGIN      
        1 ggatcgggcc gggcagcggg cgccgcagga ggcgtggcag ccgcagcggg cgccgacgat
       61 gcggcaggcc cagatggctc agttggcgca agggccggca tctggttgcc cttgcgcccc
      121 acatgcgggg ccatcggcgc cttcagcaca tcggccgagc cgcattgcgg gcacaccacc
      181 agccctgccg cctgctggct ggcaaaatcg gccgaggagc cgaaccagcc ctcgaaacgg
      241 tggccgccgg cctggcattc cagatcgaac acaatcatga cagaacggat ctaggtattt
      301 cccggcggtt ggcaagggcg ggcaattcgc gccgcacctt ggcgatgcgc tcgggcgtga
      361 tttcggcaaa ggccagcccc gccgcctcgc cgcccatgtc gagcagcact tcgccccatg
      421 ggtccaccac caggctgtgg ccataggtgg cgcggccatc ggcatgctgg cccacttgcg
      481 cggccgagac gacataggcg ctggcttcca ccgcccttgc ccgctgcagc aggtgccagt
      541 gggcgcggcc ggtgggcacg gtgaaggcgg ccggaatggc gatgagatcg cagcgcgcct
      601 gccccagcgc ctcgaacagg gcgggaaagc gcaggtcata gcagatggca aggcccagcc
      661 ggcccaccgg cgtttcagcc accaccaccg cctcgccggg cgcataggcg gccgattcgc
      721 gccagctctc gccggtttcc agatcgacat cgaacatgtg gatcttgtcg tagcgggcgc
      781 ggatctcgcc tgtgctgtcg atcacgaagc tgcggttggc ccagcggcca tcctcgcggt
      841 ccaccgccag cgagcccagc gccagccaca cgccgctttt cgccgcggcc gcgcgcaagg
      901 catccagcac cggcgaggcg ctttcgggca ccacatgggc gcgggcccgc gcgcggtcgc
      961 ggtcgatcag gcaggccatt tcgggggtga acagcatctg cgcgcccccc tcgcccgccc
     1021 gcatcagccc ttcggcaatc gtggcggcat tggcggccgg atcgatgccg ctggtcatct
     1081 gcaggagggc aatgcgggac atcggcgttt acaggcccag cgccgcgtca agcttgccgg
     1141 cggcatccag cgcatggatg tcatcacacc cgcccagcgc cacgccatcg gcgaagatct
     1201 gcggcacggt gcgcgcgccc ggcttgcgct cggccatttc ggcgcgcttt ctggtgtcca
     1261 tcgtcacgtc gatttcctca taggccacgc ccttggcatc gagcagcgcc ttggcccgcg
     1321 tgcaataagg gcagccccat tgggtgtaga tctcgacttt cgcgctcatg gttttccttt
     1381 gatcctgacg ggcttgccgc agaacggcag cacaggcgcc aagataagca cgttgccccc
     1441 ctcttgaaag acggcgcggg caacatattt ccacccctgc gccatggccc gctgcggcat
     1501 ggcgccattg caggggggcg ccgcaacagg gcccccgaaa cgccgaaaat tgctcatgca
     1561 cagaggattt ttgccatgaa ccgcttcgat ttcaccccct atcgccgcaa caccgtgggt
     1621 ttcgaccgcc tgttcgacct gctggaaaac gccacccgcg ccggcgctgg cgacaattac
     1681 ccgcccttca acatcgagcg gcgcggcgag gatacctatc gcatcacgct ggccgttgcc
     1741 ggcttcaagc ccggcgagat cgacatcacc gcccagcaga acctgctcgt tatccagggc
     1801 gccaaggccg aagatccggc cgctgccggc cattacctcc atgtcggcat tgcccagcgc
     1861 gggtttgagc gccgcttcga actggccgat ttcgtgcggg tggacagcgc cacgctggaa
     1921 gatggcctgc tggtgatcga ccttgtgcgc gaagtgcccg aggcgatgaa gcccaagaag
     1981 atcgccattg gcccggccac aacgctgagc gtggtgggcg gcgtggccgg caacggccct
     2041 gacagcgccg cggcctgaac gcatcggcca cagcgcccca aaatagacgg gggtgagccc
     2101 gggaaagctc acccccagcg gtgcgtttgc cccgcccctc tgccttgccc gccgccaggg
     2161 tcgcatgtcg cggcggttcc ggtcagagcc ccattcgccg ccagccctgc agccctttgc
     2221 cctgcggatc cacgcgcggc aaagggcagg ccaacggcgc ctttagcccg ttatgacaga
     2281 gagatgccct ggcctgcaag ccagcaatac accggatggc ccgccacctg tcttttcaac
     2341 aggcggcggg ccatccggca tcagtagagg gcaaatcaga ccagacgcgc ctggcgcagc
     2401 gcggcaccga taaagccggc aaacagcgga tgcggctcga acggccggct cttcagctcc
     2461 gggtggaact gcacgccgac aaaccacgga tgatcgggcc gctcgacgat ttccggcaac
     2521 agcccgtcgg gcgacatgcc cgagaagacc aggccgccct tttccagcgc ctcgcgatag
     2581 tggctgttca cctcgtagcg gtggcggtgg cgctcgaaga tcgtctcgct gccatagatg
     2641 ctggcgacat ggctgttgcc atccagcttc gcctcatagg cgcccagccg catcgtgccg
     2701 cccagatccg tgtcggtcga gcgggtctgc aggccttcct cgctcatcca ttcggtgatg
     2761 atgcccacca ccggctcggt ggtctcgcca aattcggtgg aggaggcatc ggcgatgccg
     2821 gccgtgttgc gcgcgccctc gatgcaggcc atctgcatgc caaggcagat gcccaggaac
     2881 ggcaccttgc gttcacgggc aaagcgcacg gcggcaatct tgccttccgt gccccgctcg
     2941 ccaaagccgc cgggcaccag aatgccgtgc agcggctcca gcgtggcggc gatctcgccg
     3001 tcgtccttct cgaagatctc ggcgtcgatc cacttgatgt ggaccttcac cttgttggcc
     3061 agccccccat gcaccagcgc ctcgttgagg ctcttgtagg catcctgcag cacgacatac
     3121 ttgccaacga cgccgatcgt cacctcgccc tcggggttgg cgtaaagctg ggtgaggttt
     3181 tcccagcggg ccagcgccgg ctcgggcgcg gccaggccaa agtgatgcag caccgccgca
     3241 tccagcccct cggcatgata ttgcaccggc acggcataga tattcggcgc atccagcgcc
     3301 gggatcaccg cttccttgcg cacgttgcag aacagggcga tcttgttgcg ctcgttctcg
     3361 ggcagcgggt gctcgcagcg gcacagcagc acatcgggct ggatgccaag gctggtcagc
     3421 tcgcgcacgg aatgctgggt gggcttggtc ttcagctcgc ccgccgcctt gatgtagggc
     3481 accagcgtca catgggcaaa gcaggtctgc tcgcggccca gctcgttgcg cagctggcgg
     3541 atcgcctcga tgaagggcag gccctcgata tcgcccaccg tgccgccaat ctcgcacagc
     3601 acgaaatcga ggtcgtcggt ttcggcctgg gcgaattcct tgatcgcgtc ggtcacatgc
     3661 gggatcacct gcaccgtggc gccgaggtaa tcgccgcgcc gttccttggc gatgatcgtc
     3721 tgatagatgc ggcccgaagt gatattatca ctcttgcgcg ccgaaacgcc ggtgaaacgt
     3781 tcgtaatgcc ccaggtcaag gtcggtctcg gcgccgtcgt cggtcacgaa gacctcgccg
     3841 tgctgatagg ggctcatcgt ccccggatcg acattgagat agggatcgaa cttgcggatg
     3901 cggaccttga agccccgtgc ctgcaacagg gccgccagcg atgccgccat gagacccttg
     3961 cccagcgagg agaccacgcc gccggtgatg aaaatgaacc gcgccatggg aattgcgcct
     4021 tagtacctta atcgggagga tgacaagcgc cagaacggcg caaaggggca gcaatccaca
     4081 actggccgtg caagacagca taaaaccgcc ccgcatgacc cggcagaaca ccgcccccct
     4141 cggatgaaca gacaggcccc gagcccttgg cgggcccgtg ttaccttttc gcgtagatcg
     4201 tgcagactcg aaaaaagcag gggcagcgcc cggccacccc ttatctgctt acggcgcccg
     4261 cttactgctt ggcggccggc gccagcggat cagccgcggc cggcgctgtg gatgccggtg
     4321 cgctgggcgc cacggtgggc gcagcgctgg gcacggctgc cggcacattg cgcaccagcg
     4381 aggtatccac cgtctcgccg ccgccgctct tcaccgccag cgccgccagc acgatcgaga
     4441 gcagcacgaa caggccggcc agcacctggg tggtgcgggt gagaaaatcg gccgcgccgc
     4501 gcgccgacat gaagccggtc gggctgccac cggtgcccag gccaccgcct tccgatttct
     4561 gcaccaggat cacggccacc atcgccgccc cgatcagggc ctggagtacg gtcaggaaga
     4621 tgaacatgga caaccttctt cagcaaatgg cttgttcagc aattgaagcg cgccacggcg
     4681 gtggcccgcc ggggcgatca acaggaacga tcaattggcg gcctctaggc gcagccgggc
     4741 cagagtgcaa ggccggttgg ggattaggcg ccccatccgg ccacgaacac gccggccagg
     4801 cgcggctcag ctctcggtga gcatggccgc ggccaccacg atcgccgcga agctttccgc
     4861 cgtgaggctg gcgccgccca ccagcgcgcc gcccacctcg tcggccgcca gcaattcgcc
     4921 ggcattggcc gcattgaccg aaccgccata caggatgcgc accttggccc cggcatcgcc
     4981 atacagcgcc accaggcggg cgcggatggc gcggtgcatg gcgcccacat ccgccaccga
     5041 gggcacgcgg ccggtgccga tcgcccacac cggctcatag gccaccgaca agcgcgcaag
     5101 cgctgcggca tcggccggca gcgagccttc gacctggccc gtcaccacct tttcggcctg
     5161 gccggcatcg cgctgctttt ccgtctcgcc cacgcagacg atgatgccca gatccgcggc
     5221 gagggctgcg tccactttgg cgcgcacatc cgaatcgctt tcgccatgca ggctgcggcg
     5281 ttccgaatgg cccaggatca cgaagctggc gccggcatcg gccagcatcg gcgccgagac
     5341 atcgccggtg aaggcgccat tagccctggc gtggcaatcc tgcccgccca ccgcgatcgc
     5401 ctgcacggcc tcggccatgc tgtggatcag cgtgaagggc ggcgccagcg ccacatccac
     5461 ctgcggcgcg cgcgtggccg cgcgatcgat cgcgcgggct tcgggcaggc tggtgcgcac
     5521 gccgttcatc ttccagttgc cgacaatata gggcctaaga gccatctgtg ttcctcgtcg
     5581 cgggccgccg atccacagac ggcggtcttg ctcatccccg gcgcgccatg ggccgcgccg
     5641 ccatgctgtc actttcgccc aaagcggcag aacgccgccg ggctgcctgt tgcggggccg
     5701 gcaaagcctg tccgggcctg tctgggcctg tcggggtctg gccggccagc ccgggcggac
     5761 accgctcgca cgcaagaatc gcgcaaggca tcctgggcca tatgcggcgg ctctagcctg
     5821 cctcgcgcgc ggtttccacc ccttgccatt gcgagaaaac gcattgccgc ctaaagggct
     5881 ggcctgggcg cgtggcgcgc attgcttccg gcgcaacact agggcgatta aatgcttcag
     5941 ttctttcgca atttcttcaa gtccacgctg ggcgtgggga tcacccttgg cctgctggtg
     6001 ctgatcgcgc tggcctttgc ggcgggcgat atcgccggca atggcgcgct cagcggcctt
     6061 ggcggggcga aggtggccac cgtgggccgc gaggccatca ccccggccca gctgaccaag
     6121 cagctgaccc aggccgctga actggcccgg caggacaatc cgcgcctgac gatgaaggaa
     6181 ttcctcgccg agggcgggct ggaggatgtg ctgtcctcga tgatcgaccg caccgccatg
     6241 gtggtgttcg gcaaggccca gggcgtggtg gccggcaagc ggctggtgga tagcgaactg
     6301 gcccgcctgc cccagctgca gaaccccgat ggcagcttca gccagagcgc ctatcgccag
     6361 ctgctggccc agcgccagct gaccgatgcc gatgtgcgcg ccgatatcgg ccaggggctg
     6421 gtggcacgcc agctgctggc cccggcagag ctggccatcg gcatgcccgc cagcatcgcc
     6481 cagcgctatg cgggcctgct taccgagcgg cgcgagggca ccgtggcggt gctgccggcc
     6541 gccctgttcg cccccaagga caagccggcc gatgccgatg tcgcccgctt ctatgccgcg
     6601 catctggcca cctataaccg gcccgagcgc cgcgtgatcc gctatgccct gttcgacgac
     6661 agcgtggcca agcaggtcgc cgcccccacc gaggccgaga tcgcccagcg ctatggcgcc
     6721 aacaaggcgc aatatgccgc ttccgaaagc cgccatatcg tgcagctgat cctgcccggc
     6781 gaggaggccg ccaaggcgct ggcggcagag ctggccaagg gccagtcgct ggaggcagcc
     6841 gccagggcgc gcggccttgc caccgcgccg ctgggcccgc tgagccacga cgcgctggcc
     6901 cagcagacct ctggcgccat tgccgatgcc gtgttcgccg cgaagaaggg cgcccttgtg
     6961 ggcccggtga agggcgcgct gggctatgcg ctgatccgcg tggacgaggt ggtgaccaag
     7021 ccggcccgca cgctcgatca ggcgcatggc gagatcgccg cgcagctggc cagcgaaaag
     7081 cgcaaggccg ccatcggcgc gctgaccgcc cgcatcgacg acaatctggg ccatggcggc
     7141 gcgctgtccg atgccgccaa ggatctcggc ctgacgctga gcgctaccgc gccgctgacc
     7201 gccgacgggc aggtctatcc cggtgcggcc ggggcgccgc tgcccacagc gctttccgcc
     7261 aaggagcagg ccagcatcgt gaaggccgcc tttgccatgg agcatgaagg ccaaccccag
     7321 ctggccgagc tggaagcggg caagcgctat gtgatctacg atgtcagcca gatcaccgct
     7381 gccgcccctg ccccgctggc cgatatccgc cagcaggtgg ccgccgatta tgcgctgacc
     7441 aagggctctg ccgccgcgca ggccgccgcg atgaacgtgc tgaaggcggt gaagggcggc
     7501 aaggatctgg ccgcaacgct gggcgggctg ggcgtgacac tgccgccggc ccagccggtg
     7561 gcgatgagcc gccagcagct gggcgccttc cagggccggg tgccgccggc gatccagctg
     7621 ctgttctcga tggccgaggg cacggtgaag ctgctgcccg cgcccggcgg ccagggctgg
     7681 ttcgtggtgc aactcaagca catccagctg ggcacgccgg cggccaatga tcccgccgtg
     7741 gcgcaggtgc gccagcagct gggccagctg gtcgcccgcg aaagcggcga gggcctgcgc
     7801 ctggcgatcc gcgccgaggc cggcaccacc cgcaacgaag ccgccatccg cgcggtcgtc
     7861 ggccagctgg tcggcggcaa ctgagcgcgc tttttcacct gcaaaccgga aacgaaaaga
     7921 acacgaaccg atgagccttt ctgccgacac tgccccgccc catgccgaga acaccgccgc
     7981 ggcccgcgcc gcgctggccg ctggcaaggg cgcgctggtc tggcgccggc tcatcgccga
     8041 taccgagacg ccggtgggca ccgcgctgaa gctgatgcgg gccgatcgcg gcgatttcct
     8101 gctggaatcg gtggaaggcg gcgaggtgcg cgggcgctac agcctgctgg gcctcgaccc
     8161 ggatctggtg ttccgcgcca ggggccatgc ggccgagatc aaccgcagct ggccccatgc
     8221 gccagaggcc ttcgcgccgc tggcgggcga tccgctggcc gaactgcgcg cgctggtggc
     8281 cgaatgccgg gtggatgtgc cggccgccct gccgccggcg ctggcctgcc tggtcggcta
     8341 tttcggctat gagacgtttg gcctggtcga gaagctgccg cagccgccgg ccaatccgct
     8401 ggccgtgccc gacatgctgt tcgtgcggcc gcgcctgctg ctggtgttcg accggctggg
     8461 cgatgagctg tttgccgtgg cgccggtgtg gccctcggcc accgcgcccg acgatgccat
     8521 taccaccgcg gccgagcgga tcgacgaggc cgcccgccag ctggccctgc cggtgccggc
     8581 ccagccgcgc ctggccgccg acacgcccct gccgacgctg acgccaacca tggccccggg
     8641 gcgctatggc gagatggtgg aacgcgcgcg tgactatatc caggccggcg acatcttcca
     8701 ggtggtgctg gcccagcgct tcaccgcgcc cttcccgctg ccgcccatgg cgctgtatcg
     8761 ggcgctcagg cgggtgaacc cctcgccctt cctctatttc ctcgatctgc ccggctttgc
     8821 cctcatcggc tccagcccgg aaatcctcgt gcgggtgcgc gatggcgagg tgaccatccg
     8881 cccgatcgcc ggcactcgcc cgcgcggcaa gacgcccgag gaagaccgcg ccaatgagga
     8941 aagcctgctg gccgacccca aggagcgcgc cgagcatctg atgctgctgg atctgggccg
     9001 caacgatgtc ggccgggtgg cggctgccgg caccgtcaag gtgaccgaca gctacacgat
     9061 cgagcgctat tcgcatgtga tgcacatcgt ctccaatgtg gtgggccggc tggataccga
     9121 cagggccgat gccatcgatg cggtgtttgc cggctttcct gccggcacgg tgtcgggcgc
     9181 gcccaaggta cgggcctgcc agatcatcgc cgagctggaa ggcgagacgc gcggcgccta
     9241 tgccggcggc gtcggctatt tcgggccgga tggcaatctc gacagctgca tcgtgctgcg
     9301 cacggccgtg ctcaaggatg gcgtgatgca tgtgcaggcc ggcgccggca tcgtcgccga
     9361 ttccgatccc gcctatgaac aacgcgagtg cgaacacaag gccggtgccc tgttcgccgc
     9421 cgcccgcgaa gccattcgcg tggccagcga agcccaattc ggacaatgac catggctgat
     9481 gacctgatcc tcgttatcga caattacgac agcttcacct ggaacctcgt ccattatgtg
     9541 atggaactgg gcgcgcgggt ggaagtggtg cgcaatgacg cgctgaccgc cgcccaggcg
     9601 ctggagaccg gcgctgccgg cttcctcctc tcgcccgggc cctgcacgcc caatgaggcg
     9661 ggcatcagcc ttgatctggt gggcgcggcc gccgatgccg gccggccgct gctgggcgtg
     9721 tgcctggggc accagtcgat cggccagtat tttggcggca aggtggtgcg cggcgggctg
     9781 atgcatggca agacgagccc tgtcacccat gatggcacgg ggctgtttgc agggctgccc
     9841 tcgcccttca cggccacgcg ctatcattcg ctgatcgtta ccgatgtgcc cgattgcctg
     9901 gtggtgaatg cccgcagcga tgatggccac acgatgggct ttcgccatgc cagcctgccg
     9961 atccatggcg tgcagttcca cccggaaagc atcgccaccg aacatggcca tgccatgctg
    10021 gccaatttcc tgaagatctg cgggatcgcg accagcctgc cggcccatcc cgtgctccac
    10081 gcatgagcag cctgcccccg gttggcgccc agccctttgg cgaggaagag gccgaggcgc
    10141 tgttcggcgc catcctcgat gcgcaggtgg aggcagaggc gatcgccgct ttcctcgtcg
    10201 ccctctccga tcgcggcgag acggcgagcg agataaccgg cgctgcccgg gccatgcgcg
    10261 cgcgcatggt gccggtggca gcgcccgcag atgtgctggc ccgcgcgatc gacgtgtgcg
    10321 gcacaggggg cgatggccac catacgctga atgtctccac cgccgtgtcg ctggtggtgg
    10381 cggcctgcgg ggtgccggtg gccaagcatg gcaaccgggc ggcctcgtcg aaatcgggcg
    10441 cggccgacac gctggaggcg ctggggctgg accttgcccg cgcgggcgag cgggccggcg
    10501 agacgctggc cgagatcggc atcgctttcc tctttgccca ggcccatcat ccaaggcttg
    10561 gccccattgc ccccattcgc cgggcgctgg gccggcgcac catcttcaac ctgatgggcc
    10621 cgctggccaa tccggccggt gtgcgccgcc agctggtggg cattgcccgg ccggcctatg
    10681 tgccgatcta tgccgatgcc atctgccggc tgggcacaga gcgcagcttc gtcgtctcgg
    10741 gcgatgaggg gctggacgag ctgtcgctgg cgggcggcaa cgaatgggcc gatgtgcagc
    10801 gcgacacggt ggcgatgaag cgcgtggcag cggccgaggc cggccttgcc gccagccccg
    10861 tcagcgccat tcgcggcggc gatgcggcgc ataatgccga tgccctgaag cgcctgctga
    10921 tgggcgagga aggcgcctat cgcgatgcgg tgctgttcaa tgcggccggc gcgctgatgg
    10981 tggcgggcga agtggccgat tggcgcgagg gcgtggaaga agcggccgag gccatcgaca
    11041 aggggctggc gaaggccttg ctcgattgct ggatttccgc cacgagggcc tgatgaccga
    11101 caagcttacc gagatctgcg acaccaagcg cggtgaagtg gccgcccgca aggcgctgac
    11161 gacactggcc gagctggacg cgcgcgccgc cacgcagagc gcgccgcgcg gcttcgaggc
    11221 ggccctgagc gccaaggccg ccaccggctt cgcgctgatc gccgagatca agaaggccag
    11281 cccgtccaag gggttgatcc gcgccgattt cgatccgccg gcccatgccc gcgcctatgc
    11341 cgaagggggc gcggcctgcc tgtcggtgtt gaccgacgcg ccctatttcc aggggcacga
    11401 ggatttcctc gtggccgcgc ggggcgcctg cgcgctgccg gtgatccgca aggatttcat
    11461 ggtggacccg tggcaggtgg ccgaagcgcg cagcattggc gccgatgcca tcctcatcat
    11521 cgttgccgcg ctggacgatg ggcagatggc cgagatcgag gcggccgcgc gccattatgg
    11581 catggatgcg ctggtggaag tgcacaatga ggccgagatg gagcgggccg cccgcctcac
    11641 ctcgcgcctc atcggcgtga ataaccgcga tctgaagcgc ttcgtcaccg atctgggcac
    11701 gaccgagcgg ctggccccgc tggcgccgga aggcacgctg ctggtggccg aaagcggcat
    11761 caacagccat gccgacctgc tgcgcctgga agcctgcggc gcgcgcacct tcctggtggg
    11821 cgaaagcctg atgcgccatg ccgatgtggc ggccgccaca agggcgctgc tgcatggctg
    11881 aactgaccca tctcgacagt gagggccagg cccgcatggt cgatgtgggg ggcaaggcgg
    11941 aaagccggcg cgttgccatc gccgaagggc gcatccgcat gaacgcggcc gcgctggaag
    12001 ccatccgcgc gggcgctgtg cccaagggcg atgtgctggc cgccgcccgc atcgccggca
    12061 tcatggccgc caagaaaacc gccgagctga tcccgctgtg ccatccgctg gcgctgtcca
    12121 gcgtggaggt ggcctgcacg ctggaggcag acggcgtgcg cgtggccgcc accgccagcc
    12181 tcaccgggcg caccggggtg gagatggaag cgctgagcgc tgccagcatc gccctgctga
    12241 cgatctatga catggccaag gcgatcgaca aggcgatggt gatcgacagc gtgcggctga
    12301 tcgagaagcg cggcggcaaa tcgggtgact ggtttgcccc ggggcaccag cccgcgccat
    12361 gagccccccc ctgctgccgc tggacgaggc gcaggcgcgc ctgcttgccc ttgccacccc
    12421 gctgccggtg gaacatgccg atatggccag cgcccttggc cattatctgg cagagccgct
    12481 gcatgcccat cgcacccagc cggcggctga tctgtccgcc atggatggct atgcggtggc
    12541 gccaggcgat ctggccggcc cctggcaggt ggtgggcgaa agcgcggccg ggcggccctt
    12601 tggcggccat ctgcaggccg gcgaggccgc gcgcgtttcc accggcgcag tgctgccggc
    12661 gggcgccggc gcggtgctgc tgcaggaaga cattacccgc agcggcgcgc atatcgcgct
    12721 gaccggcacc gcccctgccc cgcacgaccg gcacatccgc cgcgccggtt tcgattttgc
    12781 cgaggggcaa ttgctgctgc ccgccggcac gcgcatgggc gcggcgcaag tggcgctggc
    12841 gatcagcggc gggcatggcc atgtggcggt gcgccgcccg gtgcgcctgg ccatcctcga
    12901 cagcggcgac gaactggccg cccccggcca gccctgccag ccccatcaga tccccgccag
    12961 caatggcgcc atgctggccg cgctggtggc cgcgctgccc tgcgagatca cccgcgcggg
    13021 gcccgtggcc gaccggctgg aactgctggt ggcagcgctg gacgggctgg gcgcggcgga
    13081 tgtcatcgtc accagcggcg gcgcctcggt gggcgatcat gacctcatcc gcccggcgct
    13141 ggaagcctgg ggcgccgagc tggatttctg gaaagtggcg atgcggccgg gcaagccgct
    13201 gctggtggcg cggcgcgggc ggcaggtgat cctgggcctg ccgggcaacc ctgtctcggc
    13261 gctggcgacg gcctatttct tcctgcggcc gctgctgcgc aagctgctgg gggccagcca
    13321 gtgcctgccc cggccgatct gcaccacgct ggcagcgccg ctgggcgcgg gcagcgggcg
    13381 gcgcgatttt ccgcgcgggg tgtgggatgg cgccatggtg cgccccttgg gcgggcgcga
    13441 ttccagcgcg ctggccgaac tggccggcgc caattgcctg atcgaccggc caataggcca
    13501 gccagcgctg gaaacgggga aaaaggtgaa catctacctc atcgaacccc aaggatttgc
    13561 ttgaccgctt gctgattgtt gcataattgt tccttattcg ttcatggcgc cgcgcgcgcc
    13621 aagttccggg agttatgcag ccatgctcac ccgcaagcag cacgagttgc ttcgcttcat
    13681 ccaggatcgg ctggaagaaa cgggcgtttc cccctctttc gaggaaatga aggaagcgct
    13741 tgatctcaag tcgaaatcgg gggtgcatcg gttgatttcc gcgctggagg aacgcggctt
    13801 catccgccgc ctgcccaatc gcgcccgggc gctggaagtg ctgcgccagc ctgaggatgc
    13861 ggtggccccc tcgccccgcg cggcggcgcg cacgctggca acgcctgccc cggccatcgg
    13921 ccggccgctg gcgccgatgc cgcaggtcgc cgccttcacc ccggcgaatg acattgtcga
    13981 gatcccgctg catggccgca tcgccgccgg cacgccgatc gaggcgctgg agggccagtc
    14041 gatgctgccg gtgccggccg cgctgctggg cagcggcgaa cattacgcgc tggaagtgtc
    14101 gggcgattcc atggtggaag cgggcattct cgatggcgat ctggcgctga tccggcgcac
    14161 cgaagtggcg cgtgacgggg agatcgtggt ggcgctggtg cgcggcgagg aagcgacgct
    14221 gaagttcctg cgccacgagg gcggcatggt gcggctggac ccggccaatg ccgcctacga
    14281 gccgcagttc taccgcccgg ccgaagtgcg cgtgcagggc aaactggcgg ggctgctgcg
    14341 ccgctatcac tgagcgggat cggcggtgtc cgcgccgggg cgctggtctc gcggggcgat
    14401 atggggcgcg cgccaccagg gatgatcgcc ctcgctctcg ccgacgctgg caacgcggcg
    14461 gttgacgaga tcgagcgcca gcccgccggt gcgcagcagc agcacgtgat cggcgcggat
    14521 gaggcccggc gcgcaggggc cgaagatctt gccgcgcgcc accacgatat ccaccgccgc
    14581 gcaggcccgc gccaaggccg caggggccat ccgcatcgct tccttgcgca gcaccagcag
    14641 gcgccatatg cgccccccgc gcgacagttg caccaggcag gcatccggat tgcagcgcgc
    14701 gcccggccag ctctccagcg gcgccacatc gccggaaatg ccggccgctt ccagcatggt
    14761 ggcgcgggtg aaatcggatt tgccggggcg cagcagcagc aggcggccgt tgtctggctc
    14821 tgaaatggcc agattggtgc catcgccggc gatcagcaca tcgggcgggc gcaccagcgc
    14881 cagccagcca accgccaaca gcgccggcgc caaacccagc agccgcaccc gcccatgcca
    14941 cagcgccagc cacagcatcg cccccacgaa cagcgcgaag gcgccgccgc ccatcgccgg
    15001 ccccacgctc accgcatcgg ggcgggcggc ggtgaaatgg gcgatcgcca gcagcgcttc
    15061 cagcgatttg ccgcaaaccc accaggccgg cgcgccaagg cccgccagat ccagcgccag
    15121 cgccaggccg atcatcggca tcgagatgaa agtcgtcagc gggatggcca gcacattggc
    15181 catcgagcca tagatgccgg cgcggtgaaa atggtagagc gccaccggca tcagcgcgag
    15241 atcgatcacc atgccggtga gcaggatcat gaacaggtgg cggccgatgc gccaccacca
    15301 gccctcctct cgcggcgcga gaaaggcgcg ggcgggcgcg ctgtcggcca cggcgatgat
    15361 cgccagcacc gagccgaagc tcatctggaa gctggggcca acgaccgcat cgggccacag
    15421 caccatcacc accagcgcgg ccaccgccag catccgcacc gacagcggct ggcgccccag
    15481 tgccaccgcc gccagcacca gcagcgcgcc caggcacgag cgcaccgtgg gcacctgcgc
    15541 gcccgtcagc agcgtgtaga cgatgccggc gatgccgccc atgctgctgg ccaccagcgg
    15601 caggcgccag cgcagcgccg cccagggcca cagcgccagc agccgcagcg tcagcacata
    15661 aacgccgccg accaccgcgc tgacatgcag cccgctgacc gagagcagat gggtgagccc
    15721 ggcatcgcgc atggcctgat cgtcttgcgg ggtgatgccg ccgcgatcgc cgctggcaaa
    15781 ggcggcggca atcgcgcccg gcgagccggc cagttgcgcc cgcacatgtt cggccagcgc
    15841 gtgctgggtg cgggccagcc agtctggcgc cagcgccggg cgcgccagca ccaccttgcc
    15901 cagcaccgat ccggtggccg aaacgccatc gaaccaggcc gcctcggcaa agtcataggt
    15961 gccgggaaag cgcatcgggc tgggcggcat cagccgcgcg tggaaggcga tgacggcccc
    16021 ttcgaccacc cccggcgcgt ctgccgcgag gggcaaagtg agtcgcacgc ggatcaaacg
    16081 gccggtgcgc ggctctcgcg tcgcaatctc aagacgggcg cgggccaggg caggctggtc
    16141 gtagcgatcg agcaccgtgc ccaccatcac acctgcgatc tggtgggcga tgggcggcgc
    16201 gcccaccagc accgccttgc cccacacaca ggcacagccc gcagccagca tcagccccac
    16261 cccgatcacg gcctgccgca actgcggaaa acggccattt ttcggccata aggcccaggc
    16321 cagaaaggcc gccgccagcc ctgtgcagat cagccccagc cactctgcct gattggccag
    16381 aacgaaccag aatgccacgc ccgcgccaaa ggccaccacc gcccagggcg cgcggtcgaa
    16441 gccggcccct tcaagaaatt gctctacgac ttccgcagcc cttgccaagc tgccgtggct
    16501 gcgccaaggg ccgttgcgtt gcaccgcagc gcggcgcaaa aaagcacacc attccgatcc
    16561 agcgcggacg aaagaggcaa gggcgctggc gcggatataa gccatgtcgt ttattgttca
    16621 ggaaggattg gggaatggca agcgcaagcg ttgagggaga cacgggccac aagggccgcc
    16681 gcgtcgttac ccgattcgcg ccgtcaccca ccggctacct gcacattggc ggcgcgcgca
    16741 cggcgctgtt caactggctg ttcgcccgcc accatggcgg cacctatctg ctgcgcatcg
    16801 aggataccga tcgccagcgc tccaccgatg ccgcaatcga cgcgatcttc gacgggctgc
    16861 gctggctggg cctggatggc gacgagccgg cggtgttcca gttcgagcgt tccgcccgcc
    16921 atgccgaggt ggcgcaccaa ttgctggccg cgggccatgc ctatcgctgc tatctcaccc
    16981 aggaagagct ggccgccatg cgcgagaccg cccaggcgga gaagcgtccg ttccggatcc
    17041 agtcgccctg gcgcgatgcg gcagagggca gccagcccga aggcaagcct tatgtcatcc
    17101 gcatcaaggc gccccagtct ggcgagacgg tgatcaccga tctggtgcag ggcgcgatca
    17161 ccgtgcagaa caccgagctg gatgatttca tcatcctgcg ctccgatggc acgcccacct
    17221 acatgctggc cgtggtggtg gacgaccacg acatgggcgt gacccatgtg atccgcggcg
    17281 atgatcatat caacaacgcc ttccgccagc tggtgatcct gcgcgcgatg gatgcgatcg
    17341 agggtggctg gccggatccg gcctatggcc acattccgct gatccacggg gccgatggcg
    17401 ccaagctgtc gaagcggcac ggggcgctgg gcgtcgatgc ctatcgcgac gacatgggcc
    17461 tcttgccgga agcggtgatc aactatctga tgcggctggg ctggggccat ggcgacgagg
    17521 agatcatctc gcgcgcgcag gccatcgaat ggttcgacat cgtcaatgtc aacaagggcg
    17581 cctcgcgctt cgattttgcc aagctggcca acctgaacgg ccactacatc cgcgaggcag
    17641 acgatgcccg cctggccgat ctggtggcgc cgcgcatcgc tggcgacatc gaccttggcc
    17701 tgctggcccg cgccatgccg gtgctgaagc cgcgcgccaa ggatctgaac gaactggccg
    17761 atggcgccgg tttcctcttc gcgcagcgcc cgctggcgat ctcggaaaag gcagccgcgc
    17821 tgctgaccga ggatgcgcgg cagctgttgt cggatttgca tgcgctcttg cgtaaattcc
    17881 cggaaacgga atgggcgctc gaacgtttgg aagaggcgct gaaggcatat gctgcagagc
    17941 gcaatctcgg tcttggaaag ctcgcccagc cgctgcgtgc agcgctgacc gggcagacca
    18001 cctccccggg tattttcgac gttttggttt tgcttgggca agacgaggct ctggcgcgca
    18061 tcggtgcgca ggctaccctc gccacacccg gcgcagtgtg aggagaaata cagtggctga
    18121 tgtggctaag ctgagtgtcg gcgggaaaga gcttgaagct cccgtgctca agggcacggt
    18181 gggtccggat gtgatcgaca tccgcaagct gtatggccag accggggcct tcaccttcga
    18241 cccgggcttt acctcgaccg cctcttgcga aagcgcgctg acctacatcg atggcgatga
    18301 aggcgtgctt ctgcaccgcg gttatccgat cggccagctg gccgagcact cctccttcct
    18361 ggaagtgtgc tatctgctgc tgaacggcga gctgcccaac caggcggaac tgggcaagtt
    18421 caccagcacg atcacgcgcc acaccatggt gcatgagcag ctgaccaagt tcttcagcgg
    18481 tttccgccgc gatgcgcacc cgatggcggt gatgtgcggc gtggttggcg cgctgtcggc
    18541 cttctatcat gactcgaccg acatcgccga ccccgagcag cgccgcatcg ccagccaccg
    18601 cctgatcgcc aagatcccga cgatcgcggc gatggcctac aagtattcgg tcggccagcc
    18661 cttcgtctat cccgacaaca agctgtccta tgccggcaac ttcctgaaga tgaccttcgg
    18721 cgtgccggcc gaggaatatg aagtgaagcc cgccattgaa aaggcgatgg accgcatctt
    18781 catcctccat gccgaccacg agcagaacgc ctcgacctcg accgtgcgcc ttgccggctc
    18841 gtcgggcgcc aacccgtttg cctgcatcgc ggccggcatc gcctgcctgt ggggcccggc
    18901 ccatggcggc gccaatgaag ctgcgctcaa catgctgcgc gagatcggca cgcccgacaa
    18961 gatcccgcac tacatcgagc gcgccaagga caagaacgat ccgttccgtc tgatgggctt
    19021 tggccaccgc gtctacaaga actacgaccc gcgcgcgacg gtgatgcaga agaccctgcg
    19081 cgaagtgttc gatgcgctgg gcgtgaacga cccgctgttc gagaccgccc tgcgcctcga
    19141 agagctggcc ctgaacgacc cctacttcat cgagaagaag ctgttcccca acgtggactt
    19201 ctactcgggc atcatcctct cggcgatcgg cttcccgacc accatgttca ccgtgctctt
    19261 cgcgctggcc cgcaccgtgg gctgggtggc gcagtggaac gaaatgatct ccgatcccaa
    19321 ccagaagatc ggccgcccgc gtcagctcta caccggcccg gtgcagcgcg actacgtgcc
    19381 gctcggccag cgctgagcca ccggcaagag tcgccggcga gacttcacgc aaaaggcggg
    19441 ggaaggcaac ttcccccgcc ttttggcgtt tcagaccgct atcagaccgg cggcgcccgg
    19501 cctatccttg tgcgggcaga tccagcagga agcgcgcgcc ttcgcccttg cggctttcca
    19561 ccgccagttc gccgcccatg gcgcgggcca gccggcgcga gatgaacagc cccagccccg
    19621 agccaccatc gccgctgcgc cccagccgct cgaacttgtt gaacacgcgc gcctgttcct
    19681 cggcgctgag gcccggcccc tcgtcggcca ccaccagccg caccagcgcg ccggcctgcc
    19741 catattccag atgaatgcgc gagccttctg gcgcatagcg gatggcattg ccgatgaggt
    19801 tgagcaggat ctgcagcgcc cggcggaatt cggccaccgc cggcaggcgc acctcgcctt
    19861 ccggcagcag gatggcaatg cccttctgcc gcgcccgcat gcccagaatg cccgctgccc
    19921 gccgcgccac atcggccagg tcaatccggt cgggcgcggt ggtgaaatgg ggctgctcga
    19981 tcacttcgag atcggtcaaa tcgtcgatca gatcctgcag gtgctggccg gccgcggcaa
    20041 tatcggcggc atattgggcg tattcctcgg ccagcgggcc ggcaaggcgg gtgcggatgg
    20101 tttccgcatt ggcgatgatg cgggcgatgg gctggcgcag caccggcgcc agatcctgcc
    20161 ccaccagcgc cgtttccggc aggttgctgc cgggctggcc ggctttgcgc gggggcgcgg
    20221 gcagcgggcg atcggccgtg aggcacagat cgaagccggc gggctggttg gccgggccgg
    20281 caatggggat gagcgtggct cgccagcgcc gctgcgagcc agacacctcc acccgcgcgc
    20341 catccagcaa ttgccagtgc agcggctggc ggtggatatt gccgtcgatc gtgacgaaat
    20401 ccgtccacgg ccggccgatg ccgcggcgca tggcggtgga cagcgccagc aggtcgcccg
    20461 cctcgccctc caccgtcagc aggccctggt gctcgtccag ccgggcggtc agctcggcca
    20521 gcgcgcggtc gatctcggcg cggcggcggg cggccagttc cggctcctcg gcaggcacgg
    20581 gcgtggcctg ccagctgtgc agcaggatag cgcagccacc ctcggcgcgg tctgcctcgg
    20641 ggatcacttc cacccaggcg cggatcgtct cgtcgccatc ctgggcgagg atggggcgcg
    20701 ccagcttcag cccgaagcgc cgcgccttgg ccaccagctc gcgcagttcc ggcacggcaa
    20761 tggcgccggg catggtgccg ccgcagcgct gctgaaggcc ggccagaggc tcgtcggcgg
    20821 cgatcagccg gtcgttctgg tcgctggcgg cgcgggccat cggcccctgt cccaatgggc
    20881 ccatcacacc gctcctgttg ccgcgccaga ggtcagcagg cgcgccgctt tctcggcccc
    20941 cacgcgggcc agttcggcat cctcggcgct gccgccaaac agcgccaaca gcgcatcttc
    21001 caccgtggcc ggcggcgccc cccctgcccg cagcatcagc gccagacgcg tcttctgccc
    21061 ttcctgcatg gccagcacgg caagatcgcg cgcgccttgc gccccctgcg ccacggcaga
    21121 ggcaaacagc gccaccccgg catggcccgg cagcagcgcc gccagcgcgc cggcgcccat
    21181 catcgttacc agccgggcgg ccagcgcgtg gcgcgtgtgg ccctcctcat aggcttcgcg
    21241 cacggcggca gtggcaccgc cctctgcctc ccacgcggcc agcacggcat gcagcagatc
    21301 gcccggcaat tccgccagcg gccattgcat gcggctgatc tggcgggcaa agcgcgcctg
    21361 cgcggccagc agccgcatgg ccagcgccgc cacatcgggc tgggtggagc caaccagcgc
    21421 ctgcagcagc ggcgagagca ccggatcgag ccccagctgc gcctgcagcc gctgcgccac
    21481 ctgccattcc agcgcctgcg catgcagatg gcccagcagc cccggcacat cggccaggcg
    21541 cccggccagc gcgctcacgg ccgcctcgcc gccgctggca tccagcaatt ggccggcatc
    21601 cagcaactgg caggccatat cggccagcat gcccttcacc cgcgccagca catcatcggc
    21661 aaacagcgcg ccgccccgct gcatcagcaa atggcgcagc accggatcga ccgattgcaa
    21721 cacggcatcg ccgcgcgcca gctcgccgca cagcgcggcc tcggccggat cggcggcaat
    21781 ggattggaca gagccctcgt tcatccggcc atcataggag gagagcatta aagcgccgtt
    21841 agccgctttt cgcggccctc gcaggccagc gcccccacca gcagcgccag ccccagcagc
    21901 ttcaccgcac cgcccagaac gcccgccagc gccgccagcg ccagcaaggc ggcaaggcag
    21961 gcccggtctg ccgcccagcc gcccagcccg cccggcgcca gccacgccag caagcgcacc
    22021 agcccgatca gcaccagcgg cggatagacc cgcccccaca gcggatcgcc gcccgcgccc
    22081 gaccacgcca gcaccagcac cagcgcgcca tcgcccaccg ccagcaccca gcgccct
//
LOCUS       DASRYE010000339         7143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_192214,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000339 DASRYE010000000
VERSION     DASRYE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192214"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFF98_17625"
     CDS             <1..794
                     /locus_tag="VFF98_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZU65512.1"
                     /translation="AIRATQGKGLMPDGTTRFSYKGQPIFHYMGCSTFSNFTVLPEIA
                     VARIREDAPFKTSCYIGCGVTTGVGAVLNTAKVQVGDNVVVFGLGGIGLNVIQGARLA
                     GANKIIGVDLNETREEWGRRFGMTDFLNTKGMSREEIVAAIVVLTDGGADYTFDATGN
                     TEVMRTALEACHRGWGTSIIIGVAEAGKTIETRPFQLVTGRNWRGTAFGGAKGRTDVP
                     RIVDMYMAGKIEIDPMITHVMGLEEINTAFDLMHAGQSIRSVIVF"
     gene            806..1180
                     /locus_tag="VFF98_17630"
     CDS             806..1180
                     /locus_tag="VFF98_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZU65513.1"
                     /translation="MFTHCFLGTNDPARAEAFYDAVMGALGHARAPVPGVLVYPTDAG
                     ALVVGKPANGQPHVAANGFTLGFAAKSYAEVDAFHAAGLAHGGTCEGAPGHRAKAPGN
                     AYGAYLVDPDGNKICAFAPNVG"
     gene            1191..1616
                     /locus_tag="VFF98_17635"
     CDS             1191..1616
                     /locus_tag="VFF98_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZU65514.1"
                     /translation="MEADERLAAILAAHAGVEGPLLPILHEVQRVFGCVTPAAQRAIA
                     GALNLSRAEVHGVVSFYHDFTEQAEARPCVELCRAEACQARGVEALVAAAEAAAGSRV
                     KLATVYCLGLCSVGPSARVGDALYARLDAAGLVKLVEEA"
     gene            1613..3154
                     /locus_tag="VFF98_17640"
     CDS             1613..3154
                     /locus_tag="VFF98_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZU65515.1"
                     /translation="MTQVHTPRVRISDDSLALALGADALAAAFAAAGAEVERTSSWGM
                     HWLEPLVEVDGMGFGPAGPGDVAAILAGSSPLAIGRIADHPFIRPQQRLTFARAGRTR
                     PLSLADYQATGGWQGLARARAMSGAEVIAEVTQSGLRGRGGAGFPAGIKWKTVHDAPV
                     SPAHPRKYVVCNADEGDSGTFADRMLMEGDPYQLIEGLAIAAHAVGAQAAYIYTRSEY
                     PHAIAKLRAAIALAADIIAPLHMEVRAGAGAYVCGEETALLNSIEGKRGVVRAKPPLP
                     ALEGLFGCPTVVNNVLTIAAIPHIMAEGGAAEYAALGVDRSLGTKPIQLAGNIRHGGL
                     YETAFGISLHDLVYTIGGGTASGRPVKAVQVGGPLGAYLAPDQFAIPFDYEAYARADA
                     LIGHGGVVVFDDSADMGALARFAMAFCAAESCGKCTPCRIGSTRGVELIDRIRAGEPV
                     LDAVGALPQMHNAAKAPRGRAENIALLEELCETMQFGSLCALGGFAPYPVRSALKHWP
                     EDFGA"
     gene            3151..3690
                     /locus_tag="VFF98_17645"
     CDS             3151..3690
                     /locus_tag="VFF98_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZU65516.1"
                     /translation="MILGAVLAGGQSRRFGSDKALALLAGETLLARAVAQLAGWCDAV
                     VVAGRAAAPAPCVADWPAPGMGPLGGLAGALAHAGAHGFAAVLSIGVDSLGLPEDLPA
                     RLAAPPAFVAAQPVVGLWPTTALPALRALLEGDGRHSMRAFAEAIGAVSVILPAAPAN
                     INTPEDLAALQNGKMRDGL"
     gene            3680..6520
                     /gene="fdhF"
                     /locus_tag="VFF98_17650"
     CDS             3680..6520
                     /gene="fdhF"
                     /locus_tag="VFF98_17650"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZU65517.1"
                     /translation="MAYEPQADYGTPARTGAGITLTIDGRQVTVPEGTSVMRAAAEVG
                     GAIPKLCATDNVEAFGSCRMCLVEIEGMRGLPASCTTPVRQGMQVATQTPRLEKIRRG
                     VMELYISDHPLDCLTCSANNDCELQDTAAQVGLRDVRYGYAGANHLGETADTSNPYFD
                     FDPSKCIVCSRCVRACEEVQGTFALTIEGRGFGSKVSAGQKADDFLGSECVSCGACVA
                     ACPTATLNEKAVKDIGKPERAVVTTCAYCGVGCTFRAEMRGQQLVRMVPWKDGKANRG
                     HSCVKGRFAWGYANHADRITRPMIREAITQPWREVSWEEALAFTAARLGRIKAEHGAR
                     ALGGITSSRCTNEEAFLVQKLVRGGFGSNNVDTCARVCHSPTGYGLKTTFGTSAGTQD
                     FDSIEEVDTFLLIGCNPTDGHPVVASRMKKRIRQGARLIVIDPRRIDMVKSAHIAADH
                     HLALQPGTNVAVLTAMAHVIVTEGLADEAFVAERCDGEEYADWARFVADPRHAPEALE
                     PVTQVPAQDLRAAARLFATGGNGAIYYGLGVTEHSQGSSTVMAIANLAMATGNIGRRG
                     VGVNPLRGQNNVQGSCDMGSFPHELSGYRHISDGGTRALFEAEWGVTLDAEPGLRIPN
                     MLDAAVEGSFRALYIQGEDILQSDPNTHHVAAGLAAMECVIVHDLFLNETANYAHVFL
                     PGSTFLEKTGTFTNAERRIQMVRRVMDPANGYEDWEVTQALANAMGLGWDYTHASQIM
                     DEIARLTPSFTGVSHARLDAEGSLQWPVNAAHPEGSPIMHVDGFVRGKGKFVVTEYIP
                     TDEKTGPRYPLLLTTGRILSQYNVGAQTRRTANTVWHEEDVLEIHPTDAENRGLKTGD
                     WVRLASRAGETTLRAQVTDRVAPGVVYTTFHHPDTQANVVTTENSDWATNCPEYKVTA
                     VQVAPSNGPSAWQRDYAAQAAHARRIGAEAAE"
     gene            6517..6723
                     /locus_tag="VFF98_17655"
     CDS             6517..6723
                     /locus_tag="VFF98_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HZU65518.1"
                     /translation="MSAETLIRMANQIAANLAAQGEAVAIAETAQHIRDFWDPRMKAG
                     ILAADHAALSPIARAAVAQLVPAG"
     gene            6751..>7143
                     /locus_tag="VFF98_17660"
     CDS             6751..>7143
                     /locus_tag="VFF98_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HZU65519.1"
                     /translation="MSREATLALLARYYDAFNAGDNEAMLACLTEDIAHDINQGARQQ
                     GKAAFRAFLAHMDRCYKERLADIVLMAHEEGARAAAEFTVHGQYLATDEGLPEAAGQT
                     YVLPAGAFFDIADGLIARVSVYYNLADWT"
BASE COUNT         1145 a         2336 c         2515 g         1147 t
ORIGIN      
        1 ccgccatccg tgctacgcag ggcaaggggc tgatgcccga tggcaccacg cggttcagct
       61 acaagggcca gccgatcttc cactacatgg gctgctccac cttcagcaac ttcaccgtgc
      121 tgcccgagat tgccgtcgcc aggatccgcg aggacgcccc cttcaagacc agctgctaca
      181 tcggctgcgg cgtgacgacg ggcgtgggcg cggtgctgaa cacggccaag gtgcaggtgg
      241 gcgacaatgt ggtggtcttc ggcctgggcg gcattggcct caacgtgatc cagggcgcgc
      301 ggctggccgg cgccaacaag atcatcggcg tggacctgaa cgagacccgc gaggaatggg
      361 gccgccgctt cggcatgacc gacttcctga acacgaaagg catgagccgc gaggagatcg
      421 tggcggccat cgtggtgctg accgatggcg gcgcggacta caccttcgat gccaccggca
      481 acaccgaagt gatgcgcacc gcgctggaag cctgccaccg cggctggggc accagcatca
      541 tcatcggcgt ggccgaagcc ggcaagacca tcgagacgcg ccccttccag ctggtgaccg
      601 gccgcaactg gcgcggcacg gcgttcggcg gcgccaaggg ccgcaccgat gtgcccagga
      661 tcgtcgacat gtacatggcc ggcaagatcg agatcgaccc gatgatcacc cacgtcatgg
      721 gcctcgaaga aatcaacacc gccttcgatc tcatgcacgc cggccaatcc atccgctccg
      781 tcatcgtctt ttgaggggaa gcgccatgtt tacccattgt tttctgggca ccaatgatcc
      841 cgctcgcgcc gaggccttct acgatgcggt gatgggcgcg ctgggccatg cgcgcgcgcc
      901 ggtgccgggg gtgctggtct atcccaccga tgcgggcgcg ctggtggtgg gcaagccggc
      961 caatggccag ccgcatgtgg cggccaatgg ctttaccctg ggctttgccg ccaagagcta
     1021 tgccgaggtg gatgccttcc atgcggctgg ccttgcccat ggcggcactt gcgaaggcgc
     1081 gcccggccac cgggccaagg cgccgggcaa tgcctatggc gcctatcttg tcgatccgga
     1141 tggcaacaag atctgcgcct ttgcgcccaa cgtcggctga gggcagggtg atggaggctg
     1201 acgagcgcct ggccgcgatc ctggccgccc atgccggggt ggaagggccg ctgctgccga
     1261 tcctgcatga ggtgcagcgc gtgttcggct gcgtgacgcc ggccgcgcag cgcgcgattg
     1321 ccggggcgct gaacctgtcg cgcgcggaag tgcatggcgt tgtcagcttc tatcacgatt
     1381 ttaccgaaca ggcagaggcg cgcccctgcg tggaactgtg ccgcgccgaa gcctgccagg
     1441 cgcgcggcgt ggaagcgctg gtggccgcgg ccgaagcggc cgccggcagc cgggtgaagc
     1501 tggccacggt ctattgcctg ggcctgtgca gcgtgggccc ttcggcgcgg gtgggcgatg
     1561 cgctctatgc gcggctggat gcggccgggc tggtgaagct ggtggaagag gcatgacgca
     1621 ggttcatacg ccaagggtgc gcatttctga cgattcgctg gcgctggcgc tgggcgccga
     1681 tgcgctggcc gccgcctttg ccgctgccgg cgccgaggtg gagcgcacat cgagctgggg
     1741 catgcactgg ctggagccgc tggtggaggt ggacggcatg ggctttggcc cggccggccc
     1801 gggcgatgtc gccgccattc ttgccggcag cagcccgcta gccatcggcc gcattgccga
     1861 tcatcccttc atccggccgc agcagcggct cacctttgcc cgcgccggcc gcacccgccc
     1921 gctgagcctg gccgattacc aggccaccgg tggctggcag ggcctggccc gcgcgcgcgc
     1981 catgagcggc gccgaggtga tcgccgaggt gacgcagagc ggcctgcgcg gccggggcgg
     2041 ggcgggcttt cccgccggca tcaagtggaa gacggtgcat gatgcgcccg tcagcccggc
     2101 ccacccgcgc aaatatgtcg tgtgcaacgc cgatgagggc gacagcggca cctttgccga
     2161 ccgcatgctg atggagggcg acccctatca gctgatcgag gggctggcga ttgccgccca
     2221 tgccgtgggc gcgcaggccg cctatatcta cacccgcagc gaatatcccc atgccatcgc
     2281 caagctgcgc gcggcgatcg cgctggcggc cgacattatc gcgccgctgc acatggaagt
     2341 gcgcgcgggc gccggcgcct atgtctgcgg cgaggagacc gcgctgctca attcgatcga
     2401 gggcaagcgc ggggtggtgc gggccaagcc cccgctgccg gcgctggaag gcctgttcgg
     2461 ctgccccacg gtggtcaaca atgtgcttac cattgccgct atcccgcata tcatggccga
     2521 gggcggcgcg gcggaatatg cagcgctggg ggtggatcgc agccttggca ccaagccgat
     2581 ccagctggct ggcaacatcc gccatggcgg gctgtatgaa accgcctttg gcatctcgct
     2641 gcatgatctg gtctacacta tcggcggcgg cacggcatcg ggccggccgg tgaaggcggt
     2701 gcaggtgggc gggccgctgg gcgcttatct cgcgcccgat cagtttgcca ttcccttcga
     2761 ttatgaagcc tatgcccgcg ccgatgcgct gatcggccat ggcggcgtgg tggtgttcga
     2821 cgacagcgcc gacatgggcg cgctggcgcg ttttgccatg gccttttgcg cggcggaaag
     2881 ctgcggcaaa tgcaccccct gccggatcgg cagcacgcgc ggggtggagc tgatcgaccg
     2941 gattcgcgcc ggtgagccgg tgctcgatgc ggtgggcgcg ctgccgcaga tgcacaatgc
     3001 cgccaaggcg ccgcgcggcc gggcggagaa tatcgccctg ctggaagagc tgtgcgagac
     3061 gatgcagttc ggctcgctgt gcgcgctggg gggctttgcg ccctatccgg tgcgctcggc
     3121 gctgaagcac tggccggagg atttcggcgc gtgatactgg gcgcggtcct ggctggcggg
     3181 caatcgcgcc gctttggcag cgacaaggcg ctggccctgc tggcgggcga aaccctgctg
     3241 gcgcgcgcgg tggcacagct ggccggctgg tgcgatgcgg tggtggtggc cgggcgggca
     3301 gcggcgccgg cgccctgcgt ggctgattgg ccggcgccgg gcatgggccc gctgggcggg
     3361 cttgccggcg cgctggccca tgccggggcc catggctttg ccgcggtgct gagcatcggg
     3421 gtggacagcc tgggcctgcc cgaggatctg cccgcgcggc ttgcggcacc gcccgctttc
     3481 gtggcggcgc agccggtggt gggcttgtgg ccgaccactg ccctgccggc gctgcgcgcc
     3541 ctgctggaag gcgatggccg ccattccatg cgcgcttttg ccgaggcgat cggcgcggtt
     3601 tcggttatat tgccggcagc gccagccaat atcaacacgc ccgaggatct tgcggcgctg
     3661 cagaatggga agatgcgcga tggcctatga accccaggca gattacggca cgccggcccg
     3721 cacgggcgcc ggcatcaccc tgacgatcga cggccggcag gtgaccgtgc ccgagggcac
     3781 cagcgtgatg cgcgcggcgg ccgaagtggg cggcgccatt cccaagctgt gcgcgacgga
     3841 caatgtcgag gctttcggct cctgccgcat gtgcctggtg gagatcgagg ggatgcgcgg
     3901 gctgccggcc agctgcacca cgccggtgcg ccaaggcatg caggtggcca cgcaaacccc
     3961 ccggctggaa aagatccgcc gcggggtgat ggagctttac atctccgacc acccgctcga
     4021 ctgcctgacc tgcagcgcca acaatgattg cgagctgcag gacaccgccg cccaggtggg
     4081 cctgcgcgat gtgcgctatg gctatgccgg cgccaaccat ctgggcgaga ctgccgacac
     4141 ctcgaacccc tatttcgatt tcgaccccag caaatgcatc gtctgctcgc gctgcgtgcg
     4201 ggcgtgtgag gaagtgcagg gcactttcgc gctgacgatc gaggggcgcg gctttggctc
     4261 caaagtgtcg gccgggcaga aggcggatga tttccttggc tcggaatgcg tgtcctgcgg
     4321 ggcttgcgtc gccgcctgcc ccaccgccac gctgaacgag aaggcggtga aggacatcgg
     4381 caagcccgag cgcgcggtgg tgacgacctg cgcctattgc ggcgtgggct gcaccttccg
     4441 ggcggaaatg cgcggacagc agctggtgcg catggtgccg tggaaggatg gcaaggccaa
     4501 tcgcggccat tcctgcgtga aggggcgctt tgcctggggc tatgccaacc atgccgatcg
     4561 catcacccgg ccgatgatcc gcgaggcgat cacccagccc tggcgcgagg tgagctggga
     4621 ggaggcgctg gccttcaccg ctgcccgcct cggccggatc aaggcggagc atggggcaag
     4681 ggcgctgggc ggcatcactt ccagccgctg caccaatgag gaggccttcc tcgtgcagaa
     4741 gctggtgcgc ggcggctttg gcagcaacaa tgtcgatact tgcgcgcggg tctgccattc
     4801 gcccaccggc tatgggctga agaccacctt tggcaccagc gccggcacgc aggatttcga
     4861 cagcatcgag gaagtggaca ctttcctcct catcggctgc aaccccaccg atggccaccc
     4921 ggtggtggcc agccggatga agaagcgcat ccgccagggc gcgcggctga tcgtcatcga
     4981 tccgcgccgg atcgacatgg tgaagagcgc ccatatcgcc gccgatcacc atctggcgct
     5041 gcagccgggc accaatgtcg cggtgttgac ggcgatggcc catgtgatcg tcaccgaagg
     5101 gctggccgac gaggccttcg tggccgagcg gtgcgacggc gaggaatatg ccgactgggc
     5161 ccgcttcgtg gccgatccgc gccacgcgcc cgaggcgctg gagccggtga cgcaggtgcc
     5221 ggcgcaagac ttgcgggcgg ccgcgcgcct gtttgccacc ggcggcaatg gcgccatcta
     5281 ttatggcctg ggcgtgacag agcacagcca gggttcctcc accgtgatgg cgattgccaa
     5341 tctggccatg gccaccggca atatcggccg gcgcggcgtg ggggtgaacc cactgcgcgg
     5401 gcagaacaac gtgcagggca gctgcgacat gggcagcttc ccgcacgaat tgtcgggcta
     5461 tcggcacata tcggatgggg gcacccgcgc cctgttcgag gccgaatggg gcgtgacgct
     5521 ggatgcagag ccgggccttc gcatccccaa catgctggat gcggcggtgg aaggcagctt
     5581 ccgcgcgctt tacatccagg gcgaggatat tctccagtcc gatcccaaca cccatcatgt
     5641 ggcggccggc ctcgcggcga tggaatgcgt catcgtccac gacctgttcc tcaacgagac
     5701 ggccaattat gcccatgtct tcctgccggg cagcaccttc ctcgaaaaga ccggcacttt
     5761 caccaatgcc gagcgccgca tccagatggt gcgccgggtg atggatccgg ccaatggcta
     5821 tgaggactgg gaagtgacgc aggcgctggc caatgccatg gggctgggct gggattacac
     5881 ccacgccagc cagatcatgg acgagatcgc ccgccttacg ccctctttca ccggcgtgtc
     5941 gcatgccagg ctggatgccg agggcagcct gcaatggccg gtgaacgccg cgcatcccga
     6001 aggcagcccg atcatgcatg tcgatggctt tgtgcgcggc aagggcaagt tcgtcgtcac
     6061 cgaatatatc cccaccgacg agaagaccgg cccgcgctat ccgctgctgc tgacaaccgg
     6121 gcgcattctc agccagtata atgtgggcgc gcagacacgg cgcaccgcca acaccgtgtg
     6181 gcacgaggaa gacgtgctgg agatccaccc caccgatgcc gagaaccgcg gcctgaagac
     6241 cggcgactgg gtgcgcctgg ccagccgcgc gggcgagacg acgctgcgcg cgcaggtgac
     6301 cgaccgcgtg gcgcccggcg ttgtctacac caccttccac caccccgaca cgcaggccaa
     6361 tgtggtgacc accgaaaatt ccgactgggc caccaattgc cccgaataca aggtgacggc
     6421 ggtgcaggtg gcgccttcca acgggcccag cgcctggcag cgtgactatg ccgcgcaagc
     6481 cgcccacgcc cgccggatcg gcgcggaggc ggccgaatga gcgccgaaac gctgatccgc
     6541 atggccaacc agatcgccgc taacctcgcc gcgcagggcg aggcggtggc gattgccgaa
     6601 accgcgcagc atatccgcga tttctgggat ccgcggatga aggcggggat tctggcggcc
     6661 gatcatgccg ccctgtcgcc cattgcccgg gcggcggtgg cgcagctggt gcccgccggc
     6721 tgagttaccc acagaagctg aaggaccccg atgagccgtg aagccactct cgcgctgctg
     6781 gcgcgctatt acgatgcgtt caacgctggc gataatgagg ccatgctggc gtgccttacc
     6841 gaagacatcg cccatgatat caaccagggc gcccgccagc agggcaaggc ggctttccgc
     6901 gccttcctcg cccatatgga ccgctgctac aaggagcggc tggccgatat tgtgctgatg
     6961 gcgcatgagg agggcgcgcg ggcggcggcg gaattcaccg tgcatggcca gtatctggca
     7021 acggatgagg ggctgccgga agcggccggg caaacctatg tgctgcccgc cggcgccttc
     7081 ttcgacattg ccgatgggct gattgcccgc gtctcggtct actacaatct ggcggactgg
     7141 acg
//
LOCUS       DASRYE010000340         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_69312,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000340 DASRYE010000000
VERSION     DASRYE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69312"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..507
                     /locus_tag="VFF98_17665"
     CDS             121..507
                     /locus_tag="VFF98_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZU65520.1"
                     /translation="MSGDGMSEVGPAQVWQAALAEGRLLLQRPVGGGKALFPPRDAAP
                     GSGAALEWFAASGRAVLYSLTWVQQRPPAAAYAIALVELAEGPRLMARLDGLDGAGAH
                     IGMALAARITPGADGPALTFIPAGEA"
     gene            507..1658
                     /locus_tag="VFF98_17670"
     CDS             507..1658
                     /locus_tag="VFF98_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZU65521.1"
                     /translation="MADRFPRGRVAIRGLATYGLGECPGHTPIEMAARAIRLALADAG
                     LGVAAIDGLFVCLADDLFAGLSLAQYLGLHPRFTDNNRTGGASFFSHLATACLMLEAG
                     YLDCAVIAYGSNQRSGGGKLSTPARPDSWDALAAPPYPLAYYALAAARHAHQYGTTRA
                     QLAEVAVAARGWAMGNPEAFARTPLAIADCLAARMVATPLCVRDCCLVTDGAAALVLT
                     RMAEARDGPALPVPVLGVAAATWYNSLSQAEDITTTAAAESGARAMAMAGVSPAQIDV
                     LQLYDAFTINTILFLEDLGFCAKGEGGAFVEGGRIGPGGSLPVNTNGGGLSCCHPGMY
                     GLFTMVEAARQIRGSAATPVPGARIALAHGNGGTLSAQATALFGHPDTV"
     gene            complement(1684..3351)
                     /locus_tag="VFF98_17675"
     CDS             complement(1684..3351)
                     /locus_tag="VFF98_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU65522.1"
                     /translation="MADETPLRAQPCAPPIRTLGAMLRASAANWGERQAILFPDRARS
                     YAELEAAARGWARALIALGVQPGEHVGILLPTGMEFIEVFFGVAMAGAVAVPVNARYQ
                     AHELAYLVENADLVALVTTGRITATLDFCARLRAALPSLAQATGPDLLRLDEAPMLRH
                     IVSFTASDLPGILPAAEALARGQAVAPAAVDRRIDDVADEDTAMILYTSGTTADPKGA
                     LIAHRAQVANSRNLGRRYQCSAADKVWSPLPIFHIAGILPMVMILDLGGTYMTVPHFD
                     PAVALEMLGREGATIAYPSFVTIMQDLISHPAFAATDLSRLRVMNSNFAVQPAWIRDA
                     VAKAMPHTIQVGTYGLTEAAGTVSTSRLDDPYAIRTGRCGVVLEEWEMRICDPDTGAP
                     CAPGEKGEIVIRGPNMLKGYYKAPEKTAEAIRQGWFHTGDIGSVDAGGHVMFHGRTKD
                     MLKVGGENVAAAEIEAMLQSHPAVKLAQVVGVPDPRYAEVPAAFVELVEGAVASEEAL
                     IAHCRGQLASFKVPRHVRFVAAWPMSTSKIQKFRLRQQLIDELALAG"
     gene            complement(3420..>3649)
                     /locus_tag="VFF98_17680"
     CDS             complement(3420..>3649)
                     /locus_tag="VFF98_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65523.1"
                     /translation="AGAKSTSVADLWKGQPMRGGGKRSFAFFRLKPCGRDHARMLVFP
                     FRQGPCHKQGGSGAGVENKKDMNDCLFNSPV"
BASE COUNT          539 a         1312 c         1221 g          577 t
ORIGIN      
        1 cgggcccggc ccggcgctgg gggccaacag cgccgaagtg ctggccgaag ccggctttgc
       61 caaacaggag atcaccgccc tgctggacag cggcgccctg ctgggcccgg aggttccggc
      121 atgagcgggg atggcatgag cgaggttggc ccggcgcagg tctggcaggc ggcgctggcc
      181 gaagggcggc tgctgctgca gcggccggtg ggcggcggca aggccctctt ccccccgcgt
      241 gatgccgccc ccggtagcgg cgccgcgctg gaatggtttg ccgccagcgg cagggcggtg
      301 ctctacagcc tgacctgggt gcagcagcgc ccgccggccg ccgcctatgc catcgcgctg
      361 gtggaactgg ccgaggggcc ccggctgatg gcccggctgg acgggctgga cggggccggc
      421 gcgcacatcg gcatggcgct ggcggcccgc atcacccccg gcgccgatgg ccccgcgctc
      481 accttcatcc cggcaggaga ggcctgatgg cagaccgttt tccccgtggc cgcgtggcca
      541 ttcgcgggct ggccacctat ggcctgggcg aatgccccgg ccacacgccg atcgagatgg
      601 cagcgcgggc gatccgcttg gccctggccg atgccgggct gggcgtggcg gccatcgacg
      661 ggctgttcgt gtgcctggcc gacgatcttt tcgccggcct cagcctggcg caatatctgg
      721 gcctgcaccc gcgcttcacc gacaacaacc gcaccggcgg cgcgtccttc ttcagccatc
      781 tggccactgc ctgcctgatg ctggaggccg gctatctcga ttgcgcggtg atcgcctatg
      841 gctccaacca gcgctcgggc gggggcaagc tgtccacccc ggcccggccc gacagctggg
      901 acgcgctggc cgccccgccc tatccgctgg cctattacgc gctggccgcc gcccgccatg
      961 cccaccaata tggcaccacc cgcgcacagc tggccgaagt ggccgtggcc gcgcgcggct
     1021 gggcgatggg caaccccgaa gcctttgccc gcacgccgct tgccatcgcc gactgcctgg
     1081 ccgcccgcat ggtggccacg cccctttgcg tgcgcgattg ctgcctggta acagatggcg
     1141 cggccgccct tgtcctcacc cgcatggcag aagcgcgcga cgggcccgcc ctgccggtgc
     1201 cggtgctggg ggtggcggcg gccacctggt acaattcgct gagccaggcg gaggacatca
     1261 ccacgaccgc cgcggccgaa agcggcgccc gcgccatggc catggccggc gtctcgcccg
     1321 cccagatcga cgtgctgcag ctttacgacg ccttcaccat caacacgatc ctgttcctcg
     1381 aggatctggg cttttgtgcc aagggcgaag gcggcgcctt tgtcgagggc gggcggatcg
     1441 ggccgggggg cagcttgccg gtcaacacca atggcggcgg gctgtcgtgc tgccatccgg
     1501 gcatgtatgg gctgttcacc atggtggagg cagcgaggca gatcaggggc agcgccgcca
     1561 cgcccgtgcc gggcgcgcgc attgccctgg cccatggcaa tggcggcacg ctttccgccc
     1621 aggccaccgc tttgttcggc cacccggaca cggtgtgatc agccagccgg cgcccgcagg
     1681 cgctcagccg gccagcgcca gctcgtcgat cagctgctgg cgcaggcgga acttctggat
     1741 cttggaagtg gacatcggcc aggccgccac aaaacgcaca tggcgcggca ccttgaagct
     1801 ggccagctgc ccccggcaat gggcgatcag cgcctcctca ctggccacag ccccttccac
     1861 cagctccaca aaggccgccg gcacctcggc atagcgcgga tcgggcacgc ccaccacctg
     1921 cgccagcttc accgccgggt ggctttgcag catcgcctcg atctcggcgg cggccacgtt
     1981 ttcgccgccc actttcagca tgtccttggt gcggccgtgg aacatcacat ggccgcctgc
     2041 atcgacagag ccgatatcgc cggtgtggaa ccagccctgg cggatcgcct cggcggtctt
     2101 ttccggcgcc ttgtaatagc ctttcagcat gttgggcccg cgaatgacga tctcgccctt
     2161 ctcgcctggg gcgcatgggg cgccggtgtc gggatcgcag atgcgcatct cccattcctc
     2221 cagcaccacg ccgcagcggc cggtgcggat ggcataggga tcatccagcc ggctggtgga
     2281 aacggtgccg gccgcctctg tcaggccata ggtgcccacc tggatggtgt gcggcatggc
     2341 cttggccacc gcatcgcgga tccaggccgg ttgcacggcg aaattggaat tcatcaccct
     2401 caggcgcgac agatccgtgg ccgcgaaggc ggggtggctg atcagatcct gcatgatcgt
     2461 cacgaagctg ggataggcga tggtggcgcc ctcgcgcccc agcatctcca gcgccacggc
     2521 cggatcgaaa tgcggcacgg tcatgtaggt gccgcccaga tcgaggatca tcaccatcgg
     2581 caggatgccg gcgatatgaa aaatcggcag cggcgaccac accttgtcgg ccgcgctgca
     2641 ctgatagcgc cggcccagat tacggctgtt ggccacctgc gcccggtgcg caatcagcgc
     2701 gcccttggga tcggccgtgg tgcccgatgt gtagaggatc atcgcggtgt cctcgtcggc
     2761 cacatcgtcg atcctgcggt ccaccgccgc gggcgccacc gcctgcccgc gcgccagcgc
     2821 ctcggccgcg ggcaggatgc cgggcaggtc cgatgcggtg aaggagacga tgtggcgcag
     2881 catcggcgct tcgtcgaggc gcagcagatc cggccctgtg gcctgtgcca gcgagggcag
     2941 cgccgcgcgc agccgggcgc agaaatccag cgtggcggtg atcctgccgg tagtgaccag
     3001 cgccaccaga tcggcgtttt ccaccagata ggccagttca tgcgcctgat agcgggcgtt
     3061 caccggcacc gccacggcgc ccgccatggc cacgccaaag aacacctcga tgaattccat
     3121 ccccgtgggc agcaagatgc cgacatgctc gcccggctgc acgcccagcg cgatcagcgc
     3181 ccgcgcccag ccgcgcgcgg ccgcctccag ttcggcatag ctgcgggcgc gatcgggaaa
     3241 caggatggcc tgcctctcgc cccagttggc ggcagaggcg cgcagcatcg cgcccagcgt
     3301 gcggatcggc ggcgcgcacg gctgtgcccg caatggcgtc tcgtcggcca tatcctctcg
     3361 tccccggttc ttatcgggct ggccagccca tgccggccgc cccctttgtg ccgcgcagct
     3421 tacaccggcg aattaaaaag acaatcattc atgtcttttt tattttccac accagccccg
     3481 ctgccgccct gcttgtggca cggcccttgc cggaagggaa acacaagcat tcttgcatga
     3541 tcgcgcccgc aaggtttaag gcgaaaaaac gcaaagctcc tttttccgcc gccccgcatg
     3601 ggctggccct tccacaggtc tgccaccgat gtcgatttcg cgcccgcgc
//
LOCUS       DASRYE010000341        18396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_176138,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000341 DASRYE010000000
VERSION     DASRYE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 18396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18396
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176138"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..528
                     /gene="flgB"
                     /locus_tag="VFF98_17685"
     CDS             166..528
                     /gene="flgB"
                     /locus_tag="VFF98_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZU65524.1"
                     /translation="MSDPASNQSLFGIHGMALELRSQAMGLITSNIANAATPGYKARA
                     LDFNAALKAEIGGADAGQAAAAATQYRVPVMPALDGNTVEMDTEQVSFSQNAVGYETS
                     LAFLSGRIDTITRAIKGE"
     gene            533..940
                     /gene="flgC"
                     /locus_tag="VFF98_17690"
     CDS             533..940
                     /gene="flgC"
                     /locus_tag="VFF98_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZU65525.1"
                     /translation="MPGTPMSTFDLAQKAMSAQLVRMNTAASNLANAGSVASSEAAAY
                     KPLKAVFATQLDAASGLATVRVAGVVQANTPAVRQHDPGHPLADANGDIWAAPVDENA
                     EMVEMLDASRQYQNLVEAVSTTKQLMLDTVRMK"
     gene            944..1243
                     /locus_tag="VFF98_17695"
     CDS             944..1243
                     /locus_tag="VFF98_17695"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZU65526.1"
                     /translation="MTSVSSATSAASTAASTASTTSSNPYANLGMKDFLSLLTTELKD
                     QDPTQPTDNTQMVAQMATFSQLSGIQTIASNTTGLGSTLSKLASAIGGTSTTTSA"
     gene            1271..2089
                     /locus_tag="VFF98_17700"
     CDS             1271..2089
                     /locus_tag="VFF98_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HZU65527.1"
                     /translation="MSFYTSLSGIATMQTDLDVISNNLANAQTAGFKSSTATFADLVA
                     TSILTDPALTTGLGARTEKIQQNFGLGSITQTGNTTDIAINGSGFFLTKDPTSGNPVL
                     TRAGSFSVDNTGYLIDGSGARLQMFTANAAGTAPASTTTTSDIQIPTQNASGSAYSGL
                     TVSNTGVLTASYSDGTTSVVGILALANVTSTSGLKQMGAASWETTGLSGTTTYGQASS
                     GQFGSLLSGSLEGSNVNITTELVNLIAAQQDYQANSKAISTQNQITQSIMQMQS"
     gene            2113..2859
                     /gene="flgF"
                     /locus_tag="VFF98_17705"
     CDS             2113..2859
                     /gene="flgF"
                     /locus_tag="VFF98_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HZU65528.1"
                     /translation="MDRLIYTAASGMSASMIREQVIASNMANAQTTGFKAEVLYATPV
                     TVKGQGLETRALSQAEVHGANMAGGSVAATGRALDLAVQGNDLIAVQAADGSEAYTRR
                     GDLTISPSGVLQNGDGRPVIGNGGPITLPLDAQVKISPDGTVLATNAEQPNLPPSPVD
                     RIKLASPDGSSVMKGVDGLFRVAGGGVLPTNADAKVTSGALEGSNVDPSGVMVEMVQA
                     QRLFDIRTKLISTAKDVDQSGAQLMSITAG"
     gene            2897..3685
                     /gene="flgG"
                     /locus_tag="VFF98_17710"
     CDS             2897..3685
                     /gene="flgG"
                     /locus_tag="VFF98_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZU65529.1"
                     /translation="MSSSALQVARTGLEAQDTRMQVIANNLANVNTTAFKADRANFAT
                     LAYQDMRVAGQQSSSQSDFATGLNLGTGVAIQSTSRIDTQGQMQNTGNALDVAISGNG
                     YFQVLLPSGQLAYTRAGNFSLSSQGQLVTAQGYVVQPAITIPQGASSITIATDGTVSA
                     IVPGATAAQTLGQLTLASFTNPAGLQAMSDNYLQETGASGPAQVGNAGTQGRGTISQG
                     MLEQSNVNVVQELVDMIECQRAYEINSKMISSVDEMLKNANQTL"
     gene            3697..4356
                     /locus_tag="VFF98_17715"
     CDS             3697..4356
                     /locus_tag="VFF98_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZU65530.1"
                     /translation="MARPLSLALLAAALCAPMSALAGKPPKGFAPVLPDPVAPQHAPD
                     GAIFSAAQGYAPLVYGARGHGVGDIVTIALNEATNTTKTTQTKTSRSGLGSITPPTAG
                     PFAINPNALNASSTGSFNGQGSATQTDALTGTITVTIAEVRPNGTVLVRGEKRMNLSQ
                     GMEWVQFSGILRLADIDQNNTVQSTQVADARLEYAGNGTVQRAGREGWLSRFFSIFSP
                     F"
     gene            4361..5470
                     /locus_tag="VFF98_17720"
     CDS             4361..5470
                     /locus_tag="VFF98_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZU65531.1"
                     /translation="MAMRPFLSLLGALLAACVLGAMPAHADRVRDLGTFQGVRPNQLT
                     GYGVVVGLDGTGDDTLEYVTQAMRGVSGRMGLQLPQGVAPGLKNAAAVLVTAELPAFA
                     KPGQQIDITVSAIGKAKSLRGGALILTPLYGADGQIYAMAQGNLAVGGLGIAAKDGSQ
                     LSVNIPTVGRIESGATVERAVATGFDTGEVLRYNLNTADFLTAQRVRDAINTRFPGTA
                     SAEDAVTIALRLPPGADTRSSLIATIEMIDVTPADAPARVIVNSRTGTVVINSAVRLS
                     PAAISHGKLVVRIDEKTRVSQPNSFSNGQTVASPSSKISAEEEQAHVALMKGGASLSK
                     IVDALNLLGVTPSDLVAILEALRQAGALKAEMVVI"
     gene            5467..5850
                     /locus_tag="VFF98_17725"
     CDS             5467..5850
                     /locus_tag="VFF98_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HZU65532.1"
                     /translation="MSDISALAASNAANAATAPANAATAPTGAPAAGKADLKKAASQF
                     EAIFLRQMLAEARKTDFGDTLFGESGGLKTFREQQDAQFADIAANSGTLGLAKMIENQ
                     LSQRAAAGTTTTTATTAAHSAAGSR"
     gene            5861..7204
                     /gene="flgK"
                     /locus_tag="VFF98_17730"
     CDS             5861..7204
                     /gene="flgK"
                     /locus_tag="VFF98_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZU65533.1"
                     /translation="MSSDLINIALSGIKAAQAGLDVTSQNISNASTTGYVRRTIDQTD
                     LTGIDTNGAYGDLSLSGVVVTSINRNVDAFQQAEVRRTNADAARADALVTGLTNVNDA
                     IDQSGVYSSITSFQSALTTLASNPTSSSQRANVLEAAKTMAQSFNLASTSLASAISGQ
                     QFDANSDVSQINTLAQGLAQLNQKISADTDPATDNATLFDQRDQILQKLSNYTDISTT
                     IAKNGTVSVTMGGSNGTALVAGGTANTLSATTASDGTNISFALNGATITVAGGSLAAE
                     QQTLSAAATDKTALDAVASQMMTAVNTAQTGGADVNGTAGTALFTGTGAGDMAVALTS
                     GDQIVTAASGSAASSQDTTQLSSLRSALSASNGPAAAMNQLLFTTSAAVSGATTTQTA
                     LDAIATNAASQLSSAAGVNLNTEAANLVQYQQAFQASGKVIQAAQTIFEQLLNIS"
     gene            7212..8147
                     /gene="flgL"
                     /locus_tag="VFF98_17735"
     CDS             7212..8147
                     /gene="flgL"
                     /locus_tag="VFF98_17735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZU65534.1"
                     /translation="MTSVSTAAFYSSAIYNMNQLQQQTQDLQTQISTGNKLTHSYDDP
                     LAAAQMRSLQAADSLTTADTSVSNAAKTSLNLTDTALTQLSNIVTEVKTLATQAANST
                     LTDTQRAAIGTQVSQYYSQLVSLANSQDANGKALFGGNGSGSAAYTTDATGKLTYVGT
                     ASAASVSLGAGLSVTTGVTGPDVLDYSSGGSQQNLLDVVKTLGDTLSASTSSTASNTA
                     ATTAANTALTTLADGLNAITTQQTVVGARLSWIATTSSVQTAITQQRATSESDVGGTD
                     ITTAVSKLQQTMTVLEAAQASFTKVSSLSLFSLLQ"
     gene            8167..9030
                     /gene="motA"
                     /locus_tag="VFF98_17740"
     CDS             8167..9030
                     /gene="motA"
                     /locus_tag="VFF98_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HZU65535.1"
                     /translation="MYAGIGMAILFVMVFGGFAITGGALGPVFEAIPHELLIILGAAA
                     GALVTGNNLHGLKAFGGGFAKVFKGPRHNKQDHIDVIALTTKLMKLLRTEGPVAMEAH
                     VQDPAQSALFAEYPRLLANHQLSALICDTLTLIVVSSGTLEVNAVEDVMDNAMKSHFH
                     EMHEPQHALQNLADALPALGIVAAVLGVVKTMGSIDKPPSILGAMIGSALVGTFMGVL
                     LAYGIVAPMAGRLKQVIEADEQIFHAVKQVIIASLHGWPQPLVVESARSGLGHDFRPG
                     LSELLDALRGR"
     gene            9043..9897
                     /locus_tag="VFF98_17745"
     CDS             9043..9897
                     /locus_tag="VFF98_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZU65536.1"
                     /translation="MAPPKKKAEPPKPIIVKKITIVAAGHHGGAWKVAYADFVTAMMA
                     FFLLLWILGATTEKQRKGIADYFTPTLVKLREQSAGSNGLLGGSSIVDADNYPHAKGQ
                     TGTKSMTIPRDTTGGAKEGGARSKQAASMQGKLQARMNATEQLKKLARTVRITSTPEG
                     IRIDLMDDANFSMFQLGTTILTPDAVQLLRAIAGVLKDEQGGLSIRGHTDALQYRRGD
                     GATGGGTNNWSLSAGRAESTRQQLLRDGLTEDRFRRIEGVADREPLITDDPTDARNRR
                     MSILLMNL"
     gene            10080..12518
                     /locus_tag="VFF98_17750"
     CDS             10080..12518
                     /locus_tag="VFF98_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZU65537.1"
                     /translation="MTAPVAIAQFAAAGAPLQGTILPHLLPGALDLCPQARLLSLDCF
                     DTLLWRDGHAPADLFAALPGITPMQRRFAESQARRVAGFGRGRQEITIAEIYAQLLPA
                     AGPAERQAAVAAERQAAIAAELAAEARHCFAFAPTVELMRQAKARGLQVILVSDTYLD
                     AAQLAALVAAAAGAEVAGLIDRIFPSSAFGKPKALGLYGEVLRKLKVPAREILHIGDN
                     LGADVESVAPYGVNTLHLQQFPEALAQQLRLEAGYSAMLHPPQGQLAAAQPQRAALAL
                     ALPQQADAAHRLGLATIGPVLAGFDQWLRAEAAALAAARGGRVHWLFLMRDGFLPMAM
                     HTAMGASTEAPARAIEISRFTATAASFAQERDVLRFVEQETGTEARGLARQLLLPAAE
                     IEATLAGREGAEACLALLAHMRRGQVQKQSVRAARAMAERLVAHVRARANPAPGDTLM
                     LVDLGYNGSVQNRIDALLAKALGVHVAGRYLLLREVDKPGLDKRGFLSADHYDPGTLE
                     AMCLNVAVFEQLCTTTTGSVVDYTPEGEPIRAGNDIDPAQSAMRGKVQQGALAFAHAQ
                     RCASLRARQPDLLRLWREGAAATIARLMYHPLAHELAVLDAFEHDVNLGTGRKLALFD
                     RAVARRGLRQRGLFHTCGTDRMYLPAELEGQGLAPKLAFFAQQRFNLPLSFADFTDGG
                     IRLPVMFVSEEGVVPQEITAHATHDGFCMAAVPIADCRYAVAVQLGAACEWLEMDTIC
                     ALPLADVLARDAAALGHERPVAPSLDGIEQPHPRLWHCAGPASLVLIPPPPREDDTQM
                     VVRLVFRPLVMRQG"
     gene            complement(12626..13450)
                     /locus_tag="VFF98_17755"
     CDS             complement(12626..13450)
                     /locus_tag="VFF98_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZU65538.1"
                     /translation="MSVINTNISAMQAANASTTASNSLSQSMQRLSTGSQINSAADNA
                     AGLAISTSMTAQINGMNQGIKNANDGISLAQTAQGSLTEVTNMLQRVRTLAVQSANGT
                     YQSSDRTDMQSEVTSLTNQIGSVLSNTKFNGNALFSTTSGSNVTFTIQAGANSSDTVT
                     LTSAAIDGTKIDSTALDVSTAAKATTTISNVDTALSEVSATDAGLGAGQNQLQSAVNN
                     MTSDVTNLSAAKSRITDTDYSAETTSLAKAQILSQASTAMIAQANQSQQNVLSLLK"
     gene            13744..15102
                     /locus_tag="VFF98_17760"
     CDS             13744..15102
                     /locus_tag="VFF98_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZU65539.1"
                     /translation="MVDLKITAGFARRHAPLVQRAAAIIAALGEQGNLWLGDAEETPP
                     PASATSIHLARGPRCLLQRAEQRPGRPAERRLALTYTDPTSDTALAALIACATRPGAP
                     IAADPESLSVLALAERLAAADIPVLVAGPTGTGKEVLSRFIHDRSPRRTRPFVAVNCA
                     AIPEAMLEAMLFGHVRGAYTGAAQAGEGFFRAADGGTLLLDEIAEMPLALQAKLLRAL
                     QEGEVVPLGSTQPVKVDVRIIAAANRDLPTEVAEGRFRADLFYRLNVFPLALKPLRER
                     PEDVAALAFALLLRHGPATGALPWISDAALAMLRNHGWPGNVRELENVIRRALLLAEG
                     AGRLGVAEIRPEHIHFDSPARLVVPAIALPAASLALPAAPAEPTEEGFALPASHGRRR
                     LAHIVQVSEARAIMETLAACDGNRLAAARQLGISERTLRYRLASMREAGLPVARAAAR
                     GG"
     gene            15103..15483
                     /gene="fliE"
                     /locus_tag="VFF98_17765"
     CDS             15103..15483
                     /gene="fliE"
                     /locus_tag="VFF98_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZU65540.1"
                     /translation="MSGVSAIGGLSGASGIQTILALRQQIIDRSQILQQIHAGQPATA
                     TPATSSSGAASGFTGALEQALNGVNASQQNADQLSAAYETGQTQDVAKVMMARQQASV
                     GFEATLQVRNKLLSAYQDIMKMGV"
     gene            15487..17190
                     /gene="fliF"
                     /locus_tag="VFF98_17770"
     CDS             15487..17190
                     /gene="fliF"
                     /locus_tag="VFF98_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZU65541.1"
                     /translation="MAELVPATAPALSGAVHGNGAGNWLAPLTDPAGGPPMLRARAFM
                     GQPAVKKALPWFAGMATLGAAALAWGTLAPAPQRVLYTQLDDSERASVVGALDKGGVH
                     YRIDSTTGALTVDEADLYKARMLVAQNGALAMPDTASDTLDKMPMGASHALEEERLRA
                     AREHELMLSIKQIDGVMAVRVHIAEGEKSVFVRESIAPSASVMLRLADGRQLSAGQVS
                     AIVNLVAASVPGLTPDAVKVVDQEGRLLSQKNSPDADRLDMQSRLESKLQGQLAQLLT
                     PMLGDGNFTSEVQVDLDMQQLTSARETYDKQGVVRAETQQQSSQPGGQTAAGVPGVLS
                     NTPPPAPQPSAGAPQGTPSPAAGGPTPQATESSDSRTYELGRQVDVTNQGPGKIKRLS
                     VAVALSSDAMKKAKQADIDQIKQLVSAAVGADPARGDQVAVVVRAFDPLPPDKQPFYE
                     TPWFASLLHYGVPLLGVLMVLLLGVRPLIKAVKGPARAPSAAAAQPAAAGAAAADSAA
                     LPPREMGAAAPLASEEAAVDASTLERQVSLAQGLVRERPDSAVIALRQMLQPAETEPT
                     P"
     gene            17187..18203
                     /gene="fliG"
                     /locus_tag="VFF98_17775"
     CDS             17187..18203
                     /gene="fliG"
                     /locus_tag="VFF98_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZU65542.1"
                     /translation="MTTLADAPALSDAQSAAVLVMLLGEDEASRLVGQLDASELTRLG
                     ETMCALGEIGPDEILQAIDGFVARAERLGIHAHGRADQVRALMTRAVGEVKADNLMQR
                     ILPEAPTYTALELTQWLNPQAIVPLVQGEHPQAIAVLLVQLDPDVAAKVLHSLPAEQQ
                     TQVVHRVATMGPVAPEALDMLEELLSRRIELCHGRDMLTMGGTGNAAEIMNSSGKVVE
                     KRVMPEIAKIDKNLARQIESEMFKFEHLYVLDPQSMGSLLREVESDTLINALKGIAEE
                     SREVFFRAMSSRAAEGVKDEIATRGRLKMADCIEAQKQIIATARALAAEGVIAFGGGE
                     DEYV"
     gene            18196..>18396
                     /locus_tag="VFF98_17780"
     CDS             18196..>18396
                     /locus_tag="VFF98_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65543.1"
                     /translation="MSEALFSKAFADATPIALSLADLLTPPAAFTPDPRFTAPRAEAA
                     PRRWAGGRDLAPLGMADPRSEAP"
BASE COUNT         3136 a         6578 c         5910 g         2772 t
ORIGIN      
        1 tgccgcccag cgcgccgcca accgcgacgc gccttgccga aagcgccccc gcctgccccc
       61 tgccccgcca gaaaggcgtg catttccgca attggcacgc cttctgctta ttcccagcca
      121 gacccgatcc gccgctttta cgatccacca gcgaaggcca agcccatgag tgaccctgcc
      181 agtaaccaga gccttttcgg catccacggc atggcgctgg agctgcgctc gcaggcgatg
      241 ggcctcatca cctcgaacat cgccaatgcc gccacccccg gctacaaggc ccgcgcgctg
      301 gatttcaacg ccgcgctgaa ggccgagatc ggcggcgccg atgccggcca ggccgccgcc
      361 gctgccacgc aatatcgcgt gccggtgatg ccggctctgg atggcaacac ggtggaaatg
      421 gacaccgaac aggtctcctt cagccagaac gcggtgggct acgagaccag cctcgccttc
      481 ctctcgggcc gcatcgacac catcacccgc gcgatcaagg gagaataagc ctgtgcccgg
      541 cacccccatg agcacgttcg atctcgccca gaaggccatg tcggcccagc tggtgcggat
      601 gaacacggcc gcctccaacc tcgccaatgc cggctctgtc gccagcagcg aggcggcggc
      661 ctacaagccg ctgaaagcgg tgttcgccac ccagctcgat gccgccagcg gcctggccac
      721 cgtgcgcgtg gccggcgttg tgcaagccaa cacccctgcc gtgcgccagc acgatcccgg
      781 ccatccgctg gccgatgcca atggcgatat ctgggccgcc ccggtcgatg aaaatgccga
      841 gatggtcgag atgctcgatg cctcacgcca gtatcagaac ctggtcgagg ccgtttccac
      901 caccaagcaa ctgatgcttg acaccgtgag gatgaagtaa cccatgacca gcgtttcctc
      961 tgccaccagc gcggcctcga ccgccgccag caccgccagc accacctcgt ccaaccccta
     1021 tgccaacctc ggcatgaagg acttcctctc gctgctgacc accgagctga aggaccagga
     1081 ccccacccag cccaccgaca acacccagat ggtggcgcag atggccacct tctcgcagct
     1141 gtccggcatc cagaccatcg ccagcaacac caccggcctt ggcagcacgc tctcgaagct
     1201 ggcctcggcc atcggcggca ccagcaccac caccagcgcc tgatcccatc cccccaagcg
     1261 gagccagacc atgtcgttct acacctcgct ttccggcatc gccacgatgc agaccgatct
     1321 cgacgtcatc tcgaacaacc tcgccaacgc ccagaccgcc ggcttcaaga gcagcacggc
     1381 cacctttgcc gaccttgtcg ccacctcgat cctcaccgat ccggcgctga ccaccggcct
     1441 tggcgcgcgc accgagaaga tccagcagaa cttcggcctc ggctccatca cccagaccgg
     1501 caacaccacc gacatcgcca tcaacggctc gggcttcttc ctcaccaagg atcccacctc
     1561 gggcaatccg gtgctgacgc gcgcgggctc cttctcggtc gacaacaccg gctatctgat
     1621 cgatggctcg ggcgcgcggc tgcaaatgtt cacggccaat gccgccggca ccgcgcccgc
     1681 ctccaccacc accacgtccg atatccagat ccccacgcag aatgcctcgg gctcggccta
     1741 ttccggcctc accgtcagca acaccggtgt gctcaccgcc tcctattccg atggcaccac
     1801 cagcgtggtg ggcattctgg cgctggccaa tgtcacctcc acctcggggc tgaagcagat
     1861 gggcgcggcc agctgggaga ccaccggcct ttcgggcacc accacctatg gccaggccag
     1921 cagcggccag ttcggctcgc tgctgtcggg ctcgctggaa ggctcgaacg tcaacatcac
     1981 caccgagctc gtcaacctga tcgctgccca gcaggattac caggcgaact ccaaggcgat
     2041 cagcacgcaa aaccagatca cccagtcgat catgcagatg cagagctgag cggcctgagg
     2101 gggaactgag ccatggaccg cctgatctac accgccgcct cgggcatgtc ggcctcgatg
     2161 atccgcgaac aggtcatcgc cagcaacatg gccaatgcgc agaccaccgg cttcaaggcc
     2221 gaggtgctct atgcaacgcc ggtgacggtg aagggccagg gcctggaaac ccgcgccctg
     2281 tcgcaagccg aggtgcatgg cgccaacatg gcgggcggat cggtggcggc cacgggccgc
     2341 gcgctcgatc tggcggtgca gggcaacgac ctgatcgcgg tgcaggcggc cgatggctcg
     2401 gaagcctaca cgcggcgggg cgatctgacg atctcgccct cgggcgtgct gcagaacggc
     2461 gacgggcggc cggtgatcgg caatggcggg ccgatcaccc tgccgctcga tgcccaggtg
     2521 aagatctcgc ccgatggcac ggtgctggcc accaatgccg aacagcccaa cctgccgcca
     2581 agcccggttg accggatcaa gctggccagc cccgatggca gcagcgtgat gaagggcgtg
     2641 gatggcctgt tccgcgtggc cggcggcggc gtgctgccca ccaatgccga cgccaaagtg
     2701 accagcggcg cgctggaagg ctcgaatgtc gatcccagcg gggtgatggt ggagatggtg
     2761 caggcccagc gcctgttcga catccgcacc aagctgattt ccaccgccaa ggacgtcgac
     2821 cagtccggcg cccagctgat gagcattacg gccggctgag cctgccgccc cccttttcgc
     2881 aaccaggagt ttgcccatgt cctcttccgc cttgcaggtg gcccgcaccg gcctcgaagc
     2941 gcaggatacg cgcatgcagg tcatcgccaa caatctggcg aatgtgaaca ccaccgcctt
     3001 caaggcggat cgcgccaatt tcgccacgct ggcctatcag gacatgcgcg tggccggcca
     3061 gcaatcctcc tcgcagagcg attttgccac agggctgaac cttggcaccg gcgttgccat
     3121 ccagtccacc agccggatcg acacgcaagg ccagatgcag aacaccggca atgcgctgga
     3181 tgtggcgatc tcgggcaatg gctatttcca ggtgctgctg ccctcgggcc agctggccta
     3241 cacgcgggcg ggcaatttct cgctgtcctc gcaaggccag ctggtcacgg cgcagggcta
     3301 tgtggtgcag ccggcgatca ccatccccca gggcgccagc tcgatcacca tcgccaccga
     3361 tggcaccgtc tcggcgatcg tgcccggcgc caccgccgcg cagacgctgg gccagctgac
     3421 cctggccagc ttcaccaacc cggccggcct gcaggcgatg agcgacaact acctgcagga
     3481 aaccggcgcc agtggcccgg cgcaagtggg caatgccggc acccaggggc gcggcaccat
     3541 cagccagggc atgctcgaac agtcgaacgt caatgtcgtg caggaactgg tcgacatgat
     3601 cgagtgccag cgcgcctacg aaatcaattc caagatgatc tcgtcggtcg acgagatgct
     3661 gaaaaacgcc aaccagacgc tgtgaggaac aagcctgtgg cccgccccct ttcccttgcc
     3721 ctgctggccg ccgccctgtg cgcgccgatg tcggcgctgg ccggcaagcc gcccaagggc
     3781 tttgcccccg tgctgcccga tccggtggcg ccccagcatg cccccgatgg cgccattttc
     3841 agcgcggcgc agggttatgc gccgctggtg tatggcgcgc gcgggcatgg ggtgggcgat
     3901 atcgtcacca tcgccctcaa cgaggcgacc aacaccacca agaccaccca gaccaaaacc
     3961 agccgcagcg gccttggcag catcaccccg cccaccgccg gccccttcgc catcaacccc
     4021 aatgccctta atgcttcgag caccgggtcg ttcaatggtc agggcagtgc aacgcaaacc
     4081 gacgcgctga ccggcaccat caccgtcacc atcgccgagg tcaggcccaa tggcactgtc
     4141 cttgtgcgcg gagaaaagcg catgaacctc tctcagggca tggaatgggt ccagttctcg
     4201 gggatcctgc gcctggccga tatcgaccag aacaacaccg tgcaatccac ccaggtggcc
     4261 gatgcgcggc tggaatatgc cggcaatggc accgtgcagc gcgccgggcg cgagggctgg
     4321 ctgtcgcgct tcttctccat cttcagcccg ttctgaggcg atggcgatgc gccccttcct
     4381 ctccctgctt ggcgccctgc tggcggcctg cgtgctgggc gccatgcccg cccatgccga
     4441 ccgcgtgcgc gaccttggca ccttccaggg cgtgcgcccc aaccagctga ccggctatgg
     4501 cgtcgtggtg ggcctcgatg gcacgggcga tgacacgctg gaatatgtga cgcaggccat
     4561 gcgcggcgtt tccgggcgga tggggctgca attgccgcaa ggggtggcac ccggcctcaa
     4621 gaacgccgcc gccgtgctgg tgacggccga gctgcccgcc tttgccaagc ccggccagca
     4681 gatcgacatt accgtctcgg cgatcggcaa ggccaaatcg ctgcgcggcg gcgcgctgat
     4741 cctcaccccg ctgtatggcg ccgacgggca gatctatgcg atggcgcagg gcaacctggc
     4801 cgtgggcggc ctgggcattg ccgccaagga tggctcgcag ctgtcggtca acattccaac
     4861 ggtgggccgc atcgaaagcg gcgccacggt ggaacgggcc gtggccaccg gcttcgatac
     4921 cggcgaggtg ctgcgctaca acctcaacac cgccgatttc ctcaccgccc agcgcgtgcg
     4981 cgatgcgatc aacacgcgct ttcccggcac cgcttcggcc gaggatgcgg tgaccatcgc
     5041 cctgcgcctg ccgcccggcg ccgacacccg ttccagcctg atcgccacca tcgagatgat
     5101 cgatgtgacg cccgccgatg cgcccgcccg ggtcatcgtt aacagccgca ccggcacggt
     5161 ggtgatcaac agcgccgtgc gcctcagccc ggcggcgatc agccacggca agctggtggt
     5221 gcgcatcgac gagaagaccc gcgtcagcca gcccaattct ttcagcaacg gccagaccgt
     5281 ggccagccct tccagcaaga tcagcgccga ggaggaacag gcccatgtgg cgctgatgaa
     5341 gggcggcgcc tcgctgtcga agattgtcga tgcgctgaac ctgctggggg tcacgccctc
     5401 ggatctggtg gccattctgg aagccctgcg ccaggccggc gcgctgaaag cggagatggt
     5461 ggtgatatga gcgacatctc cgcccttgcg gccagcaatg ccgccaacgc cgccacggcg
     5521 ccagctaacg ccgccacggc gcccaccggc gcccctgccg ccggcaaggc cgatctcaag
     5581 aaggcggcca gccagttcga ggcgatcttc ctgcgccaga tgctggccga agcgcgcaag
     5641 accgattttg gcgacacgct gtttggcgaa agcggcggcc tcaagacctt ccgcgagcag
     5701 caggatgcgc aatttgccga tatcgccgcc aattccggca cgctggggct ggcaaaaatg
     5761 atcgagaacc agctgagcca gcgcgccgcc gccggcacca ccaccacaac cgccacaacc
     5821 gccgcgcaca gcgccgccgg cagccgttga ggagccagac atgtcgtcag acctcatcaa
     5881 tatcgcgctc agcggcatca aagcggcgca ggccgggctc gatgtcacct cgcagaacat
     5941 ctcgaacgcc tcgaccaccg gctatgtgcg ccgcacgatc gaccagaccg acctcaccgg
     6001 catcgacacc aacggcgcct atggcgatct gtcgctgtcg ggcgtggtgg tcaccagcat
     6061 caaccgcaat gtcgatgcct tccagcaggc cgaggtgcgc cgcaccaatg ccgatgccgc
     6121 ccgcgccgat gcgctggtga caggcctcac caatgtgaac gatgcgatcg accagtcggg
     6181 cgtctattcc tcgatcacca gcttccagtc ggcgctgaca acgctggcca gcaaccccac
     6241 cagttcctcg cagcgggcca atgtgctgga agcggccaaa accatggcgc aaagcttcaa
     6301 cctggccagc acctcgcttg ccagcgccat ttccggccag cagttcgatg ccaacagcga
     6361 tgtcagccag atcaacacgc tggcgcaggg cctggcccag ctgaaccaga agatctctgc
     6421 cgataccgac ccggcgaccg acaatgccac cctcttcgac cagcgcgacc agatcctcca
     6481 gaagctgagc aattacaccg atatctccac caccatcgcc aagaacggca cggtgagcgt
     6541 gacgatgggc ggcagcaatg gcaccgcgct ggtggcaggc ggcacggcca acacgctgtc
     6601 ggccaccact gccagcgatg gcaccaacat ctcctttgcg ctgaacggcg ccaccatcac
     6661 ggtggccggc ggatcgctgg cggccgagca gcagaccctg tctgccgcgg ccaccgacaa
     6721 gaccgcgctc gatgccgtcg ccagccagat gatgacggcc gtgaacaccg cccagaccgg
     6781 cggcgccgat gtgaacggca cggccggcac cgccctgttc accggcaccg gcgccggcga
     6841 catggccgtg gcgctgacct ctggcgacca gatcgtcacc gccgcttccg gctctgcggc
     6901 cagcagccag gataccaccc agctctccag cctgcgcagc gccctttcgg ccagcaatgg
     6961 cccggccgcg gcaatgaacc agctcttgtt caccacgtct gcggcggtat cgggcgccac
     7021 caccacccag accgcgcttg atgccattgc caccaatgcg gccagccagc tgtcgtcggc
     7081 agccggcgtc aacctcaaca ccgaggcggc caatctggtg cagtatcagc aggccttcca
     7141 ggcctcgggc aaggtcatcc aggccgccca gaccatcttc gaacaactcc tcaacatctc
     7201 gtgaggccga catgaccagc gtgagcacag ccgccttcta ttcctcggcc atctacaaca
     7261 tgaaccagct gcagcagcag acgcaggacc tgcagacgca gatcagcacc ggcaacaagc
     7321 tgacccattc ctacgacgac ccgctggccg ccgcccagat gcgcagcctg caggccgccg
     7381 attcgctgac caccgccgac acctccgtgt cgaacgcggc caagaccagc ctcaacctca
     7441 ccgataccgc gctgacccag ctgtcgaata tcgtcaccga ggtgaagacg ctggccacgc
     7501 aagcggccaa ctccaccctg accgacaccc agcgcgccgc catcggcacc caggtcagcc
     7561 agtattacag ccagctcgtc tcgctggcca acagccagga tgccaatggc aaggcgctgt
     7621 tcggcggcaa tggcagcggc agcgcggcct acaccaccga tgccaccggc aagctcacct
     7681 atgtgggcac ggccagcgct gccagcgtgt cgctgggggc gggcctgtcg gtgaccaccg
     7741 gcgtgaccgg gcccgacgtg ctcgactaca gctcgggcgg cagccagcag aacctgctgg
     7801 atgtggtgaa gacgctgggc gacacgctgt cggcctccac cagttccacc gccagcaaca
     7861 ccgcggccac caccgccgcc aataccgccc ttaccaccct ggccgacggg ctgaacgcca
     7921 tcaccaccca gcagacggtg gtgggcgccc gactgagctg gattgccacc acctccagcg
     7981 tacagaccgc catcacccag cagcgcgcca ccagcgaaag cgatgtgggc ggcaccgaca
     8041 tcaccaccgc cgtctcgaaa ctgcagcaga cgatgaccgt gctggaagcg gcccaggcca
     8101 gcttcaccaa ggtctccagc ctgtcgctgt tcagcctgct ccagtaagcg caaaggaacc
     8161 ccgtccatgt atgccggcat tggcatggcc attctctttg tcatggtctt cggcggcttt
     8221 gccatcactg gcggcgcgct ggggcccgtg ttcgaggcga tcccgcacga gttgctgatc
     8281 attctggggg cggcggccgg cgcgctggtc accggcaaca acctgcatgg gctgaaggcc
     8341 tttggcggcg gctttgccaa ggtgttcaag gggccccgcc acaacaagca ggatcacatc
     8401 gacgtgatcg cgctgaccac caagctgatg aagctgctgc gcaccgaagg gccggtggcc
     8461 atggaagcgc atgtgcagga cccggcgcaa tcggcgctgt ttgccgaata tccgcggctg
     8521 ctggccaatc accagctctc cgccctcatc tgcgacacgc tgacgctgat cgtcgtctct
     8581 tcgggcacgc tggaagtgaa tgcggtggaa gatgtgatgg acaatgccat gaaaagccat
     8641 ttccacgaga tgcacgagcc ccagcacgcc ctgcagaacc tggccgatgc gctgccggcg
     8701 ctgggcattg tggcggcggt gctgggcgtg gtgaagacga tgggctcgat cgacaagccg
     8761 cccagcatcc tgggcgcgat gatcggctcg gcgctggtgg gcacgttcat gggcgtgctg
     8821 ctggcctatg gcatcgtggc gccgatggcc ggccgcctga agcaagtgat cgaggccgac
     8881 gagcagatct tccatgccgt gaagcaggtg atcatcgcct ccttgcatgg ctggccgcag
     8941 ccgctggtgg tggaaagcgc gcgttcgggg ctggggcatg atttccgccc gggcctgtcc
     9001 gaactgctcg acgcgctgcg cggccgctga ggaccacccc ccatggcccc ccccaagaaa
     9061 aaggcggaac cgcccaagcc gatcatcgtc aagaagatca ccattgtcgc tgccggccat
     9121 catggcggcg cctggaaggt ggcctatgcc gatttcgtga cggcgatgat ggccttcttc
     9181 ctgctgctgt ggatcctggg cgccaccacc gagaagcagc gcaagggcat tgccgattat
     9241 ttcaccccca cgctggtgaa gctgcgcgag caatcggccg gctccaacgg gctgctgggc
     9301 ggctcgtcga tcgtcgatgc cgacaactac ccccacgcca aggggcagac cggcaccaaa
     9361 tccatgacca tcccgcgcga taccacgggc ggcgccaagg agggcggcgc gcgctccaag
     9421 caggcggcca gcatgcaggg caagctgcag gcgcgcatga acgccaccga gcagctgaag
     9481 aagctggccc gcaccgtgcg catcacctcc acccccgagg gcatccgcat cgacctgatg
     9541 gacgatgcca atttctcgat gttccagctg ggcaccacca tcctcacgcc cgatgcggtg
     9601 caattgctgc gcgccattgc cggcgtgctg aaggacgagc agggcggcct ttccatccgc
     9661 ggccacaccg atgccctgca ataccggcgc ggcgatggcg ccaccggcgg cggcaccaac
     9721 aactggtcgc tttcggccgg gcgagcggaa tccacccgcc agcagctgct gcgcgacggg
     9781 ctgaccgagg accgcttccg ccgtatcgag ggcgtggccg accgcgagcc gctgatcacc
     9841 gacgacccca ccgatgcccg caaccggcgc atgtcgatcc tgctgatgaa cctgtgaggg
     9901 gagcgcggcg cgctgcccta agggaaaacc cctgggcgcc tggcggggaa gctttcaccc
     9961 tgcccgttta ccaatagggc cgaccgggcc tgcgaggtgc ctgccgggcg ttcgccgctc
    10021 cctccttgcc gcctgctgcc cctcgcggcc cggcacagca cgagggaagc ccgcctgcga
    10081 tgaccgcgcc tgttgccatt gcccagtttg ccgccgccgg cgccccgctt cagggcacca
    10141 tcctgcccca tctgctgcct ggcgcgctgg atctgtgccc gcaggcccgc ctcctcagcc
    10201 tcgattgctt cgacacgctg ctgtggcgcg atggccatgc gcccgccgat ctgtttgccg
    10261 ccctgcccgg catcaccccc atgcagcgcc gctttgccga atcgcaggcg cgccgggtgg
    10321 ccggctttgg ccggggccgc caggaaataa cgatcgccga gatctacgcg caattgctgc
    10381 ccgctgccgg cccggccgag cggcaggcgg ccgttgccgc tgaacgacag gcagcgatcg
    10441 ccgccgaact ggccgccgag gcgcgccact gcttcgcctt tgcccccacg gtggagctga
    10501 tgcggcaggc caaggcgcgc gggctgcaag tcatcctcgt ctccgacacc tatctcgatg
    10561 ccgcccagct ggccgcgctg gtggcagcgg ccgccggcgc cgaagtggcc ggcctcatcg
    10621 accgcatttt cccctcctcg gctttcggca agcccaaggc gctgggcctg tatggcgagg
    10681 tgctgcgcaa gctgaaggtg cccgcgcgcg agatcctcca cattggcgac aacctcggcg
    10741 ccgatgtcga aagcgtggcc ccctatggcg tcaacacgct gcacctgcag cagtttcccg
    10801 aggcgctggc gcagcaattg cggctggaag cgggctattc ggccatgctg cacccgccgc
    10861 agggccagct ggccgccgcc cagccgcagc gcgcggccct cgccctggcg ctgccccagc
    10921 aggccgatgc cgcccaccgg ctgggccttg ccaccatcgg cccggtgctg gccggcttcg
    10981 accagtggct gcgcgccgag gcggccgccc ttgccgcagc gcgcggcggc cgtgtgcact
    11041 ggctgttcct gatgcgcgat ggcttcctgc ccatggccat gcacacggca atgggcgcca
    11101 gcacagaggc cccggcccgc gccatcgaga tcagccgctt caccgccacc gccgccagct
    11161 ttgcccagga acgcgacgtg ctgcgctttg tggagcagga aaccggcacc gaggcgcgcg
    11221 gcctggcccg ccagctgctg ctgcccgcag ccgagatcga ggccacgctg gccggccgcg
    11281 aaggggccga ggcctgcctg gcgctgctgg cccacatgcg ccggggccag gtgcagaagc
    11341 aatccgtgcg cgccgcccgc gccatggccg aacggctggt ggcgcatgtg cgcgcccgcg
    11401 ccaaccccgc ccccggcgac acgctgatgc tggtcgatct gggctataac ggcagcgtgc
    11461 agaaccgcat cgacgcgctg ctggccaagg cgctgggggt gcatgtggcg ggccgctatc
    11521 tgctgctgcg cgaggtggac aagcccggcc tcgacaagcg cggcttcctg tccgccgacc
    11581 attacgaccc cggcacgctg gaagccatgt gcctgaacgt ggcggtgttc gagcaattgt
    11641 gcaccaccac gaccggatcg gtcgtcgatt acacgcccga gggcgagccg atccgcgccg
    11701 gcaacgatat cgatccggcc cagagcgcca tgcgcggcaa ggtgcagcag ggcgcgctgg
    11761 cttttgccca tgcccagcgc tgcgccagcc tgcgcgcgcg ccagcccgac ctgctgcgcc
    11821 tgtggcgcga aggcgcggcg gccacgatcg cccggctgat gtatcacccg ctggcgcacg
    11881 aactggccgt gctggacgcc ttcgagcatg atgtgaacct gggcaccggg cgcaagctgg
    11941 ccctgttcga cagggccgtg gccaggcgcg gcctgcgcca gcgcggcctg ttccacacct
    12001 gcggcacgga tcgcatgtat ctgcctgccg agctggaagg gcaggggctg gcgcccaagc
    12061 tggccttctt tgcccagcag cgcttcaacc tgccgctcag ctttgccgat ttcaccgatg
    12121 gcggcatccg cctgccggtg atgttcgtca gcgaggaggg cgtggtgccg caggagatca
    12181 ccgcccatgc cacgcatgac ggcttctgca tggccgccgt gcccatcgcc gattgccgct
    12241 atgcggtggc ggtgcagctg ggcgcggcct gcgaatggct ggagatggat acgatctgcg
    12301 ccctgccgct ggccgatgtg ctggcccggg atgccgccgc gctgggccat gaacgccccg
    12361 tggcgcccag cctcgatggc atagagcagc cccatccgcg cctgtggcac tgcgccggcc
    12421 ccgccagcct ggtgctgatc cccccgcccc cgcgcgagga tgacacgcag atggtggtgc
    12481 ggctggtgtt ccgcccgctg gtgatgcgcc agggctgaac cgccccccgc tttcccccgc
    12541 gcgcaggcag acggcccgca caccggaaaa gcccggcgac cgcatggccg ccgggctcct
    12601 ccaggacagg ggtgtcgtct tgcgcttact tcagcagcga gagcacgttc tgctggctct
    12661 ggttggcctg ggcgatcatc gcggtggaag cctgcgagag gatctgggcc ttggccaggc
    12721 tggtggtttc ggccgaatag tcggtgtcgg tgatgcgcga cttggcggcc gacaggttgg
    12781 tcacgtcgct ggtcatgttg ttcacggccg actgcagctg gttctggccg gcgcccaggc
    12841 ccgcatccgt tgccgaaact tccgacaggg cggtgtcgac attgctgatc gtcgtcgtcg
    12901 ccttggccgc ggtcgacacg tcgagcgccg tcgagtcaat cttggtgcca tcgatcgcgg
    12961 ccgaggtcag cgtcaccgtg tcggagctgt tggcgccggc ctggatggtg aaggtcacgt
    13021 tcgagcccga ggtggtggag aacagcgcat tgccgttgaa cttggtgttc gacagcaccg
    13081 agccgatctg attcgtcagc gaggtcactt ccgactgcat gtcggtgcgg tccgagctct
    13141 gataggtgcc gttggccgac tgcacggcca gcgtgcgcac gcgctgcagc atgttggtca
    13201 cctcggtcag cgagccctgc gcggtctggg cgaggctgat gccgtcgttg gcgttcttga
    13261 tgccctggtt catgccgttg atctgggcgg tcatcgaggt ggagatggcc aggcccgcgg
    13321 cattgtcggc ggccgagttg atctgcgagc cggtcgacag gcgctgcatc gactgcgaca
    13381 gcgagttgga agccgtggtg gaggcattgg cggcctgcat ggcgctgatg ttggtgttga
    13441 tgacagacat ggtgtaactc ccgttcaagt cccgaagaag ccagcctgtc ggcccatccg
    13501 acgtggctgc catccacaag atcggccggc cgaaacgaaa tttaagggcg gcaaattcat
    13561 gccgcacccc ttgtttgtgc cggcgcaaac caccaccggc agcaactagg ggaataaacc
    13621 tatccccttg aaaaaacgac atgtttaagt gattctacga ggacttaatt ttcctgctcc
    13681 tttgacgttt acgcgccatc ttccagccaa cgaccgaaca actatccagg gacgttcgac
    13741 tcaatggttg acctcaagat caccgccggg ttcgcccggc gccatgcccc gcttgtgcag
    13801 cgcgcggcag ccatcatcgc cgctctggga gagcagggga acctgtggct gggcgatgcg
    13861 gaagaaaccc cgccgcccgc cagcgccacc tccatccacc tggcgcgcgg cccccgctgc
    13921 ctgctgcagc gcgccgaaca gcgccccggc cgcccggccg agcggcgcct ggccctcacc
    13981 tacaccgacc ccaccagcga tacggcgctg gccgcgctga tcgcctgcgc cacccggccc
    14041 ggcgcgccga tcgccgccga tccggaaagc ctttcggtgc tggcgctggc cgagcggctg
    14101 gccgcggccg atatccccgt gctggtggcc gggcccaccg gcaccgggaa ggaagtgctc
    14161 agccgcttca tccatgatcg cagcccgcgc cggaccaggc cgtttgtggc ggtcaactgc
    14221 gcggcgatcc ccgaggcgat gctggaggcc atgctgttcg gccatgtgcg cggcgcctac
    14281 accggcgcgg cccaggccgg cgagggcttc ttccgcgcgg ccgatggcgg cacgctgctg
    14341 ctcgacgaga ttgccgagat gccgctggcg ctgcaggcca agctgctgcg cgcgctgcag
    14401 gaaggcgaag tggtgccgct gggctccacc cagccggtga aggtggatgt gcgcattatc
    14461 gccgcagcca accgcgacct gccgaccgaa gtggccgagg gccgcttccg cgccgacctg
    14521 ttctatcggc tgaacgtctt cccgctggcg ctgaagccct tgcgtgaacg gcccgaggat
    14581 gtggcggcgc tcgcctttgc gctgctgctg cgccatggcc cggccaccgg cgccctgccg
    14641 tggatttccg atgccgcgct ggcgatgctg cgcaaccatg gctggccggg caatgtgcgc
    14701 gagctggaaa atgtgatccg ccgcgcgctg ctgctggccg aaggcgccgg ccggctgggc
    14761 gtggccgaaa tccgcccgga acatatccat ttcgacagcc ccgcgcggct ggtggtgccg
    14821 gcgatcgcgc tgcccgcggc cagcctcgcc ctgcccgccg cccccgccga gcccacggaa
    14881 gaaggctttg ccctgcccgc cagccatggc cggcggcgcc tggcccatat cgtgcaggtg
    14941 tcggaagcgc gggcgatcat ggaaacgctg gcggcctgcg atggcaaccg gctggccgcc
    15001 gcccgccagc tgggcatttc cgagcgcacc ttgcgctatc gcctggcctc catgcgcgag
    15061 gccggcctgc cggtggcgcg cgctgccgca agggggggct aaatgagcgg ggtgagcgcc
    15121 atcggcggcc tctcgggcgc cagcggtatc cagacgatcc tggccctgcg ccagcagatc
    15181 atcgaccgct cgcagatcct gcagcagatc cacgccggcc agcctgccac cgccacgcct
    15241 gccaccagca gcagcggcgc ggccagcggc tttaccggcg cgctggagca ggcgctgaac
    15301 ggcgtcaacg ccagccagca gaacgccgac cagctgtccg ccgcctatga gaccggccag
    15361 acgcaggatg tcgccaaggt gatgatggcc cgccagcagg cctcggtcgg cttcgaggcc
    15421 accttgcagg tgcgcaacaa gctgttgagc gcctaccagg atatcatgaa gatgggggtt
    15481 tgatagatgg ccgagcttgt tcccgccacc gcccctgccc tttccggcgc cgttcatggc
    15541 aatggcgccg gcaactggct ggccccgctg accgatccgg ccggcggccc gccgatgctg
    15601 cgcgcgcgcg ccttcatggg ccagccggcc gtgaagaagg cgctgccctg gtttgccggg
    15661 atggccacgc tgggcgcggc cgcgctggcc tggggcacgc tggccccggc cccgcagcgc
    15721 gtgctctaca cccagctgga tgacagcgag cgcgccagcg tggtcggcgc actcgacaag
    15781 ggcggggtgc attatcgcat cgacagcacc accggcgccc tcacggtgga cgaggcagac
    15841 ctttacaagg cgcgcatgct ggtggcccag aacggcgcgc tggcgatgcc cgacaccgct
    15901 tccgacacgc tcgacaagat gccgatgggg gccagccacg cgctggagga agaacgcctg
    15961 cgcgccgcgc gcgagcatga gctgatgctc tcgatcaagc agatcgacgg ggtgatggcg
    16021 gtgcgcgtgc atattgccga gggcgagaaa tccgtcttcg tgcgcgagag catcgcccct
    16081 tccgcctcgg tgatgctgcg gctggccgat ggccggcaat tgtcggcagg ccaggtcagc
    16141 gcgatcgtca atctggtggc ggcctctgtg cccggcctca cgcccgatgc ggtgaaggtg
    16201 gtcgatcagg aggggcgcct gctctcgcag aagaattcgc ccgatgccga ccggctcgac
    16261 atgcagtcgc gccttgaaag caagctgcag ggccagctgg cgcaattgct gacgccgatg
    16321 ctgggcgatg gcaatttcac cagcgaggtg caggtcgatc tcgacatgca gcagctgacc
    16381 agcgcgcgcg agacctatga caagcagggc gtggtgcgcg ccgaaaccca gcagcaatcc
    16441 agccagcccg gcggccagac ggcggccggc gtgccgggcg tgctgtcgaa cacgccgccg
    16501 cctgcgcccc agccctcggc cggcgcgccg cagggcacgc ccagccccgc tgccggcggc
    16561 cccacccccc aggccaccga aagctcggac agccgcacct acgagctggg ccggcaggtg
    16621 gatgtgacca accagggccc gggcaagatc aagcgcctgt cggtagcggt ggcgctgtcg
    16681 tcggatgcga tgaagaaggc caagcaggcc gatatcgacc agatcaagca gctggtgtcg
    16741 gcagcggtgg gggcagatcc ggcccgcggc gatcaggtgg cggtggtggt gcgcgccttc
    16801 gatccgctgc cgcccgacaa gcagcccttc tatgaaacgc cgtggtttgc cagcctgctg
    16861 cattatggcg tgccgctgct gggcgtgctg atggtgctgc tgctgggcgt gcgcccgctg
    16921 atcaaggcgg tgaagggccc ggcccgggcg ccgtccgccg ccgccgccca gcccgccgcc
    16981 gcaggggccg ccgctgccga cagcgccgcc ctgcccccgc gcgaaatggg cgccgccgcc
    17041 ccgctggcca gcgaggaagc cgcggtcgat gcctccacgc tggagcggca ggtgagcctg
    17101 gcccaggggc tggtgcgcga gcggcccgat agcgcggtga ttgccctgcg ccagatgctg
    17161 cagcccgccg agacggagcc cacgccatga ccacgcttgc cgatgccccc gccctttccg
    17221 atgcccaatc ggccgccgtg ctggtgatgc tgctgggcga ggacgaagcc agccgcctgg
    17281 ttggccagct ggatgccagc gagctgaccc ggctgggcga gacgatgtgc gcgctgggcg
    17341 agatcggccc cgacgaaatc ctgcaggcga tcgacggttt cgtcgcccgc gccgaacgcc
    17401 tgggcatcca cgcccatggc cgcgccgatc aggtgcgcgc gctgatgacc cgcgcggtgg
    17461 gcgaggtgaa ggccgacaac ctgatgcagc gcatcctgcc cgaggccccc acctacaccg
    17521 cgctggaatt gacccagtgg ctgaacccgc aggcgatcgt gccgctggtg cagggcgaac
    17581 atccgcaggc gatcgccgtg ctgctggtgc agctggatcc ggatgtggcc gccaaggtgc
    17641 tgcacagcct gcccgccgaa cagcagaccc aggtggtgca tcgcgtggcc accatggggc
    17701 cggtggcgcc cgaggcgctg gacatgctgg aagagctgct ctcgcgccgg atcgagctgt
    17761 gccatggccg cgacatgctg accatgggcg gcaccggcaa tgccgccgaa atcatgaaca
    17821 gttcgggcaa ggtggtggaa aagcgggtga tgcccgagat cgccaagatc gacaagaacc
    17881 tcgccaggca gatcgagagc gagatgttca agttcgagca tctctatgtg ctcgatccgc
    17941 aatccatggg ctcgctgctg cgcgaggtgg aaagcgacac gctgatcaac gcgctgaagg
    18001 gcattgccga ggagagccgc gaggtgttct tccgcgccat gtcgagccgc gcggccgaag
    18061 gcgtgaagga cgagatcgcc acgcgcggcc ggctgaagat ggccgattgc atcgaggcgc
    18121 aaaagcagat catcgccacc gcccgcgcgc tggcggccga aggggtgatc gcctttggcg
    18181 gcggcgagga tgaatatgtc tgaggcgctg ttttccaagg cctttgccga tgccacgcca
    18241 atcgccctga gcctggccga tctgctgacg ccgccggccg ccttcacgcc cgatccgcgc
    18301 tttaccgcac ctcgggcaga ggcagcgccc cggcgctggg ccggcgggcg agatctggcg
    18361 ccgctgggca tggcagaccc ccgcagcgag gcgcct
//
LOCUS       DASRYE010000342        32685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_192934,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000342 DASRYE010000000
VERSION     DASRYE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 32685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32685
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192934"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1266)
                     /locus_tag="VFF98_17785"
     CDS             complement(190..1266)
                     /locus_tag="VFF98_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZU65544.1"
                     /translation="MSDIFSPVQLGDIALANRIVMAPMTRDRAGEGDVPTPIMAEYYR
                     QRASAGLIITEGTQPSPAGKGYWRTPGIHAPAQIAGWAQVADAVHGAGGRIVMQIMHC
                     GRASVRANKAADADTIAPSAIASPDRIPGPDGVPVPPEMPRAIATGEIPALIAQFAQA
                     ARNACAAGMDGVELHCASGYLPMQFLSSNTNQRSDAYGGSAANRVRFVVEVLAAMAGA
                     IGPGRVGLRICPGVTFNGMADADPAETHATLLQAIAGMGLAYVHLIHIPNQGFDALEL
                     VRAHWPGAVIENNALSFERAGALLASGGAEAVSFGYPFIANPDLVARFRAGAPLAKAD
                     RAHFYTGEGDDRRGYTDYPALAEA"
     gene            complement(1271..1903)
                     /locus_tag="VFF98_17790"
     CDS             complement(1271..1903)
                     /locus_tag="VFF98_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65545.1"
                     /translation="MTDTSALLARIDRLESLDAIRQLPAKYALALDMRDADAWVNLFP
                     EDVKVGGGQHGRKALRDWFDDTHSRQFTGTSHHIGGHIIDFDDADHAQGIVYSKNEHE
                     AGGDWVIMQMMYFDKYERIGGRWHFRRRLPLYWYAADLNRPPIGERKMRWPGVPPYEG
                     SFHDLFPSWKTYWERGGTAHDGPVDPPAPLEKFIETMRRGAPLPRPRVRS"
     gene            complement(1947..4262)
                     /locus_tag="VFF98_17795"
     CDS             complement(1947..4262)
                     /locus_tag="VFF98_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65546.1"
                     /translation="MKRLLATTALSAGLVAGAQAALADSPAPAADPANQTGIGEIVVT
                     AQKRAENIQQVPIAITAVGSQFLQSRGITSIEGLGTMAPDLTIERAPGDKTITQIAIR
                     GSVTINPAVTWEPAVGLYLNGVYIAKAQGSIFDVADLERVEVLRGPQGTLYGRNALAG
                     AINLIPRAPSGKLEGRAEVTYGNYNYWKERVILDLPAWGAFSLKLSGQLQSRGGFVQL
                     SPQGHTDTLNGKSGMVQLRYHPAGSRLTADYMFDYTEYNQQPQFAQLYHLNANGDPRD
                     IFTNPAASGYAGGYFPLAGYLNTQRQSVAALDAPEYERSRTYGHALTVALDLGAATLK
                     STTATRSMHWTDSLDLDGSPLPVALTVRDTYFRSFSQELQLTGKALDARLSYVAGLFY
                     YYEKAFTYNPQTYFAGSAVFDSNYGSHTRALAAYTQADYAFTERLKLTLGARFTHEQK
                     DITRFYEVVNDPSIGITSPTVLANIPYGAVPDAKYDSFTPAATLAFRPLRDINLYARY
                     AKGFKSGGFNGETSTFTTPTAQCPSGAPELCNPYKPETVDSFEIGAKTKWLGGKVIFN
                     VAGFWDEHKNIQLSVFTAQGAAASAVLNAAKARIRGLEFETVLRPTSALTISGSLAYL
                     DAHYESFIDGGVDVSNNRAFPHAPHYTASLNADARLARGHWGELHLMGDLSYVSSYFT
                     YPYALATSSPSDQSAYTSEAPARTLVNLRASVMQIPLGFVKADVSAWVKNLTNYAKPA
                     NYIDFGPGFGGLTVAYFTDPRTYGLSVGVKF"
     gene            complement(4385..5437)
                     /locus_tag="VFF98_17800"
     CDS             complement(4385..5437)
                     /locus_tag="VFF98_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZU65547.1"
                     /translation="MLTYPAIEPPVIGDGSAAAVMPGLSWLRMPIAGSLAAINVWALA
                     DGDGWTVVDTGLHTPDTVQAWRRAMEGPLAAGVPRAIVTHQHPDHAGMAGWLASRHGT
                     QLAMSPLEYYMLRALVPELGHGEPPELRAFYRAAGWSEAELDEVLAGLGAFGQIVAPL
                     PSQFAALREGQRLTIGARSWEVLMGQGHSPQHALLHCAADGLLISGDQVLPDISSNVS
                     VYPQEPAADPLSDWLASLAAIRRRVPDDTLVLPAHGRPFRGLHARIDALIDGHERGLA
                     RLHARLTEPQRAVDVFGALFRQKIGPRTLQMATGECLAHLACLGTRGLATCAPDAAGI
                     GWWQAAPAPAPHADNA"
     gene            complement(5463..6620)
                     /locus_tag="VFF98_17805"
     CDS             complement(5463..6620)
                     /locus_tag="VFF98_17805"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZU65548.1"
                     /translation="MSKPPLADLRILDFSALLPGAVCSLWLADLGAEVIKIEPPVHGE
                     AARGPAGTPPGGLFHLTNRNKKSLALDLKEAAAIATLHQLVESADAVLESFRPGVMAR
                     LGLDYPTLRAINPRLVYCSLTGYGQSGPLAARAGHDINYQAVAGVLGQNVVDGSRPSP
                     GSTPLADMAGGGLSAAVAMLAALFDAQRSGIGRHIDIAMADCTMALNLMPLAGQMLRG
                     GQAPVPGCDLLSGGLPCYRTYATADGRHLAVGALEHKFWRAFCQAIGRPDLEPRGWDM
                     GEQLAATVAEIAATIASKTLAEWQAILADIDACATPVPSLAEALAHPQAQARAMVIEV
                     AGGGATTTQYALPIRMSDFAFAVQLPPPHIGQHNGEILAALAALAGPASHT"
     gene            complement(6625..7851)
                     /locus_tag="VFF98_17810"
     CDS             complement(6625..7851)
                     /locus_tag="VFF98_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU65549.1"
                     /translation="MDFNDTPAEAAFRRQVRAWLDTHAHPRRSREDAFGAGLDPAAYL
                     AAARAWQAGKAAAGYAAITWPQAMGGRGGTPAQQAIYDEEEARFLVPGHVFEVSLGTA
                     LPTVAMWGTPAQKQRHIAPALRGEQIWCQLFSEPAAGSDIGNLRMRARREGDGWILSG
                     QKVWTSGAHFADFGVLLARTDPDRPKTRGLTMFLLDMRAPGVEVRPIRQASDEAHFNE
                     VFLTDVWIPDSDRLGPEGEGWKVMLGTLMQERFSLGRNFAGDLHAELIGLARTAQWGG
                     RPAIEDARVRAGIAHTWLSQFGVELIVRRGASAISRGQSPGPEMAAIKLVGARAFQTG
                     GALAIELGGAEGLIAAADLGPRWAHPQRLWLAAPGARIAGGTDEIQKNTIAERVLGLP
                     GEIRTDRDVPFRQLEG"
     gene            complement(7851..8957)
                     /locus_tag="VFF98_17815"
     CDS             complement(7851..8957)
                     /locus_tag="VFF98_17815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZU65550.1"
                     /translation="MHFDFSEDQRRLQDELRRMLGAISTPAEVRRVLEGTACHSAASW
                     RALADIGALGLALPEALGGAGMGRLELCLLAEEAGRALAAVPLLGTTYLAAELLLHAA
                     SPALQAQYLPRIAAGTAILTAAIDQHGQHAGPTPAPLLAAHTLTAQLDAVADGMLAEA
                     IIVLAGDALVLVDLAGPGVQRCPRASLDPTRPLAQIACTAAPAQVLCPAGGRAIAARI
                     SDGAAVLLAFEQVGGAEKALYAARDHALERRAFGRVIGGFQAVRHKLADIYIAVELAR
                     AHAYYGAWALATNAPELPRAAAAARVAATDAYTLAAEESLHLHGGIGFTWEMDCHLHL
                     RRARWLGQILGTRHYWEERLAAALIAEAGQEEAA"
     gene            complement(8972..11143)
                     /locus_tag="VFF98_17820"
     CDS             complement(8972..11143)
                     /locus_tag="VFF98_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZU65551.1"
                     /translation="MNQAITTSIDADGICTICMDLPGEKMNTLGPALTDPLEAALRAA
                     MADPAVKGIVLASGKDSFVAGGDLKQIGKGVDLAGKRPEEIVHLFSYLSRLLRWMETA
                     GKPVACAVGGLALGGGLEIAMACHHRVVTDDPRIQLGLPESMVGLIPGGGGSQRLPRL
                     VGLAKALPMMMEGRSVGPAEALAMGLVDAVVPAARLIATAKAWLRETGDAVQPWDRKG
                     FCVPGGAGSTDAGFRNLFIGANALTRAKGFGNYPAPEKLLAALYEGLQLPIDKALGVE
                     AKYLAQLMMNPVAGNLIRTLFVNKGRADALAARPAGIAALPCARIGVVGAGTMGAGIA
                     LCAAQAGIEVVLLDRDMASAGKGKAYAEKRLQRAIEKGRGTPARAAASLARICPTTDY
                     ADLAEATLVIESVFENRAVKEAVIARIAAAVADTALIASNTSGLPISGLAASAGRPER
                     FIGLHFFSPVERMGLVEIIRGAQTDATCWAHALDFVGRLRKTPITVNDGPGFFTTRFI
                     GAFVSASLAMLEEGVKPALIENGARMLGMPMGALTISDTIGLDVSYHAGATQAKERGT
                     AHHAGIIGTLYQAGRHGAKNGMGFFDYEADGGKRLWPGLGALLPQLARQPAIGEVKAR
                     LLHAQLAEGARCFAEGVLVDATDGDLGATLGVGFPSWLGGPFAAMDTLGLAAVIAECD
                     RLCAAHGALYALPDLLRAMAAAGQTFHGDRAAPSPGARRAA"
     gene            complement(11158..12372)
                     /locus_tag="VFF98_17825"
     CDS             complement(11158..12372)
                     /locus_tag="VFF98_17825"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZU65552.1"
                     /translation="MRDTFIYDHLRTPRGRGKPDGALHEATPVELVRQLLAALKSRNT
                     LPDDALDEVVLGTVIAAGEQGATIARIAPLLAGFADSTPGLQINRACASALEAVSLVA
                     AKIGCGMADLAIAGGVEAMSRIPMGADGGGPHGIDPRVSFHTPFVMQGISADLLATLN
                     GYGRADLDAYALASHQRAAQAWAEGRFARSVLPVRDMMGTVLLAQDEHIRADATPATL
                     AALKPAFAGLGRSMGFDALAIQRYPEVDRIEHVHTAASSSGIVDGAGLMLVGSAEAGA
                     RLGLRPRARIRAFVSVGSEPALMLDGPTPACARALAIAGLAPADIELWELNEAFAAVP
                     LRLMQTMNIPHDRMNVNGGAIAMGHPLGASGAMIAGTLIDEMERRGLGLGLVSLCAAG
                     GMGIAMVLERVQ"
     gene            complement(12500..13198)
                     /locus_tag="VFF98_17830"
     CDS             complement(12500..13198)
                     /locus_tag="VFF98_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU65553.1"
                     /translation="MTQNASDTTGAPGAPPQGKRAQAIAERRRRLVKAASELIAERDE
                     GAFSMPELAARAGLSLATPYNLFGSKAAVLGEVFARQVRGFHRDNGWMEEADPVARIL
                     GVVDRLVAAYAREPRFFRNLWKAFHTLSPGEHHQMGVPGGGHLLAPLVHSLAASGALI
                     ADVPPALVEITLIRLFEANFEQWALQGWDIDQLRAGLQASFALVFLGLLDTPCRAPLE
                     ATIRAAAPVLAPPQ"
     gene            complement(13293..14105)
                     /locus_tag="VFF98_17835"
     CDS             complement(13293..14105)
                     /locus_tag="VFF98_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65554.1"
                     /translation="MGGRLQGQGAVVTGAGRGIGQAIVRRLAAEGAQVAALDLQPGAA
                     ADSGAALALACDVTSSASVAAALAEARTAFGRIDIAVHNAGIGRAAQDGSDAFYAAMA
                     RRHAEIAEHGRSDTIVDQLIHMEDEGWAAVMAVNLDGAFRLARAFTRLLAQDGHGGSF
                     IALSSTSAQSGEGSAHYCASKAGIIGLVRQLARELAPRGIRVNAVAPGPTDTPVMQGI
                     PAEWITAMQQAIPLGRMADPAEIAAAVAFLASADASFVTGSVLAANGGSYFL"
     gene            complement(14105..14896)
                     /locus_tag="VFF98_17840"
     CDS             complement(14105..14896)
                     /locus_tag="VFF98_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65555.1"
                     /translation="MADHTPTPRPLAGQVAIITGGSDGIGLATAALLARRGAHVAICG
                     RRQELLDKAAAQIGAEGGSVEAIRLDVTDFDALAAMIADVASRHGRLDMLVNNAMSTH
                     YAPIGRLELSHWRKDFAVNADAVFVATKAAMAIMAGQGKGAIVNIASTCGIRAAPFMS
                     SYSASKAALIQFTAVAAMEGARQGIRVNAIVPGQVQTAGTEDFAAKAPEIATRTAEAI
                     PMGRGGQPEELAEAIAFMLSDAASYITGTALPVDGGKAAQLYMPS"
     gene            15231..16097
                     /locus_tag="VFF98_17845"
     CDS             15231..16097
                     /locus_tag="VFF98_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65556.1"
                     /translation="MHKVALVTGASRGAGRGIARGLGELGYTVYVTGRTLSPGDARGW
                     DGSVLPGTVSETAAEVTAAGGRGIAVVCDHADDAQVAALFARIAADEGRLDVLVNNAT
                     YIHPQLIEKMPFWEKDLDAVKILEVGLRSAYVASWHAARIMVPQGSGVIAFGSSFGGS
                     CYMHGPAYGAQKAGVDKLAHDMEHDLRGTGVVAVSIWMGPLVTERSRIAAQTHPEQYE
                     GFIETAENPEFTGHIIDAIARAPERDTLSGQTLIAAEIARQLGVTDRGQERPSYREML
                     GAPRPKNPAAVY"
     gene            16099..17691
                     /locus_tag="VFF98_17850"
     CDS             16099..17691
                     /locus_tag="VFF98_17850"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZU65557.1"
                     /translation="MAGSFHLADLFETVARTVPGRIAIEGDTRCLTYAGLDERATRLA
                     TGLAAQGLGRGDTVGLYLTNRVEFIEAFLAVIKLGAIPFNVNYRYRAEELRYLFANGR
                     AAAIIHDAQYGEILRGLRGDLADLKLCVAVADGSLEAAAGSLAYADLLESAPAGPWPR
                     AEDDIMLTYTGGTTGMPKGVMWPHRAFFFACAGGGGHFNPHGPATCPQDIADRAAHGY
                     PLRMFTVAPLMHAAALWTVWSALLNGLTVVLDESGRFDAAAIWQKVADRNVQLLQIVG
                     DAMAIPLRDALRAHPGRWDLSGLVSLGSGGALFSRHVKEDLAGLLPPAVRLTDGMGST
                     ETGVAGMAAASAEGMMQLEAGETLKVVVDDRFAVAGETGIIARSGHTPVGYFGDAEKT
                     AEVFRQIDGRLWVISGDSGRLEADGRITVFGRGSTCINSGGEKIFPEEVEEALRAHPA
                     IRDAVVAAQADARWGERVVGIVSLRAGHAAPALEEMRGFLGDALAGYKLPRALVWVDE
                     VHRSPAGKQDYRWARAMAETAR"
     gene            17688..19367
                     /locus_tag="VFF98_17855"
     CDS             17688..19367
                     /locus_tag="VFF98_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZU65558.1"
                     /translation="MSAHDAAFAALTAPDAPFAIVERDGMRQFAHAPADLEAMIEGAR
                     RHGDKTFIVEEAGDGSLRRLTFAEVFAWRDELAAMLHIHRGERVAICMRNRAEWIVAF
                     LAVVKAGGIAALLNSRGSPAELVAMVHEVTPELVLADTERATLLRAGGYTGRLFDLTR
                     PLAPEEIARRSAVPAELPAPASGEDACAILFTSGTTGRVKGAVLSHRNLITGLMGVQL
                     SGMQVLHTMAAAMGQPMEALLARMPQQAVLLVYPLFHISGLGSAFLSPLLAGSKVVIM
                     RRWDADVALRLIREEQITLFTGVPTMLWDVLHKAGGGAGDLASVRNVASGGQALPVNL
                     LAQMRAACPQAVMGTGYGMTECAGAIAQAVGEEFVRKPASAGRVLPLVEARIVSPQGA
                     VLPAGEAGEILVRGAQVMRGYWNRPEDTAAVLAPDGWLHTGDVGMIDEEGYIFIVDRK
                     KDMVISGGENIYCAEVERVLGEMPQVTECAAFGIPDERLGELLVAVVRPGADGRGPDA
                     AAVIAWVGERLARYKAPAHVAFVTETLPRNHVGKVDKIALRAAWPTLYGEK"
     gene            19364..20239
                     /locus_tag="VFF98_17860"
     CDS             19364..20239
                     /locus_tag="VFF98_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZU65559.1"
                     /translation="MTMGMPDNIRVIDCMMGLPEAEDRAEWFAPFRPLIKDAQTLQQF
                     AMPAQYMFKDIPQSGQQDDYIRWTVEQMDRFNVERALVGWNDNATSRRARELYGDRFF
                     FDLPCDPNRGMEEVRRIKRIHAEVGLAAISVFPAGTLPQVAINHKYMFPLYACAEELG
                     LPVLLNVGIPGPRIPMETQKVEHLDEVCWFFPDLKVVMRHGAEPWEALAVKLMLKWPN
                     LYYSTSAFAPKHYPKAIIDYANTRGADKIIYAGYFPMGLSLDRIFADMQHVPLKDEVW
                     PKFLRENARRVFNLA"
     gene            20266..21378
                     /locus_tag="VFF98_17865"
     CDS             20266..21378
                     /locus_tag="VFF98_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU65560.1"
                     /translation="MSNSDTAATDARTPPVAVWQLLEGLKGQDLADWRLAPVDGRASV
                     AERNLDIGFPFGWYPIELSAQLAPGEVKPLRYFARDLVMWRGEDGQARIMDAWCKHLG
                     AHMGHGGKVHGNLLECPFHAWRYDGDEGVVKEIPYSRAIPPQVKRQCTRTYAVTEANR
                     WIWMWYHPEGIAPLFDVAVIPECADPEWTDFEVHEWNVWGNVQNMAENGVDVAHFRYI
                     HGTANVPLGDLRWGEWGRGADVKAKMGTPWGEVDGCISYDTMGPGQSWTRFTGISETL
                     LMACITPVELDHAHTRFCFTQPRAQAEGERAGIARAIIRDICKQYDQDKVVWDRQKFE
                     PNPIICEGDGPIAQFRKFYARYYVEGEGRPPVRAQG"
     gene            21383..22816
                     /locus_tag="VFF98_17870"
     CDS             21383..22816
                     /locus_tag="VFF98_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZU65561.1"
                     /translation="MAGASPLDTQDALGLAGLVRRGEVAPLELLEEAIGRAEALDPQF
                     HFLAQRHYDYGRAAIARGLPQGPFTGVPWLLKDLNTHIAGLPSEGGSRFYQGQLAAET
                     SELVRRIERAGFVVFGKTTTPEFGLSGTTENRLTGATRNPWNPAHIAGGSSGGAAVAV
                     AAGVLPAAHATDGGGSIRIPAACCGLFGLKPSRGRVPMGPPRSEGWGGLATHHAITRS
                     VRDSAAILDATWGAEPGARYAAPTPERPFLAEVTRPPRALRIALMTAPFAGSPVAAPC
                     VAATRAAAALCERLGHHVEEAAPAIDGAALGAASFAILGGSIAADVADRAAATGLDPA
                     ALLEPITLAFIAYGKGFSAMDYARANATFQAAALTMARFLETYDVILSPTLASPPLAL
                     GEISLSPGVGMDVWGQRAGAFAAFTGIANMTGQPAMSLPLAMSDGGLPIGVQVLGRYG
                     EEGLLLRLAGQIEQAAPWVLRRPPAAA"
     gene            22813..23127
                     /locus_tag="VFF98_17875"
     CDS             22813..23127
                     /locus_tag="VFF98_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65562.1"
                     /translation="MSERMGVLAGALGLLGLFGLAGLAAVRQPCAPGRAGARVRALGL
                     LGLGGIAGFWIDGAGAMGAAGALSLWNHQNLRLARWAWPGWSFLAGAAVVLARLPELA
                     QG"
     gene            complement(23140..23919)
                     /locus_tag="VFF98_17880"
     CDS             complement(23140..23919)
                     /locus_tag="VFF98_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZU65563.1"
                     /translation="MTGTLAGRTALITGAGDGIGRAMAQRMAAQGASVIVAEYDAARG
                     NACAQDIRAAGHQARFLPVDVTDRAAVLAMMAEAGPVDILVNNAWASQGMARIEAKSD
                     AVFEASLRMALFAAKWTMEAALPHMKAQHWGRVINMASLNGVNAHMGSADYNAAKEAM
                     RAYTRTAAREWAPLGICCNMICPAAMAASTRLVMAGQPGMIERIHAANPMGRLGDPAA
                     DIAGVAVFLASEDARYLTGNTLFVDGGAHINGVAWAPDLED"
     gene            24045..24803
                     /locus_tag="VFF98_17885"
     CDS             24045..24803
                     /locus_tag="VFF98_17885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65564.1"
                     /translation="MRGLQGKVAIVAGGGRGIGAATARRLAEEGAAVVIGDLLVDLAG
                     EVASTIVAAGGRALAVPLDGTDAASQGAIVAAACSHFGGLDIYHSNLAGGTEGDIDAL
                     NCPVEVLEKSFAINTKSHFLATQAALPALLERGGGAMIYTSSGAAAAGAPWQVAYPMT
                     KNAIHALARHVAAKWGKQGIRANVICPGLVLTEAAQMHLTDAYREQGLKAIPHKRLGK
                     AEDIAAAVTFLASEDGEWVNGQVWHVNGGNIKRD"
     gene            complement(24850..25602)
                     /locus_tag="VFF98_17890"
     CDS             complement(24850..25602)
                     /locus_tag="VFF98_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65565.1"
                     /translation="MTYDFDGKVAVVTGASGGIGRATAIAFAASGAAVVVSDVNDAGG
                     AETVEMITAAGGKALYQHCNVGDAAEVKALMARAEAAYGRLDFAHNNAGINRIGSDEY
                     QDEAWALSLSVNLSGVMYCMREQSAIMLRQGKGAIVNTASINGLVGNGMQPAYTATKH
                     GVVGLTRHGALRWAKGGIRVNAVCPGVIETPMTAPLAANPDIRKTMDSLTPLGRMGEA
                     REIAAAVLWLCSDEASFVTGHPLVVDGGATAF"
     gene            complement(25737..26816)
                     /locus_tag="VFF98_17895"
     CDS             complement(25737..26816)
                     /locus_tag="VFF98_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZU65566.1"
                     /translation="MATTADYGLGPHTFPRGWFMIAQSSDIADKPVPLRFFGREFVAY
                     RGQSGKAYLVDAYCPHMRVHIGRNTTSYIVRDGNQIDGEAIRCPGHGWRFTPEGQCDD
                     IPYATRGVPKAACIKTFPVAERAGCLWMWHDEEGAAPEFDLPAFAEWDGETAGWVRWR
                     IDALGTLPVHPQEILDNMADIAHFIPVHGSRDILYFENEFADHVIMQRFGSGHRTLVS
                     DTAAVLATDTWYTGPGILLSRMEGEHPSVIMICHTPVEDGMVRVWYGLMVKVADGPAD
                     AAGIAMARSYQDVALEAFAQDFELWQHKEPALSILQIPDDGPFHKERIWYRQFYHPRA
                     RAGEFRDRVNGIHATIDRRPGARAA"
     gene            complement(26983..27480)
                     /locus_tag="VFF98_17900"
     CDS             complement(26983..27480)
                     /locus_tag="VFF98_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU65567.1"
                     /translation="MALVQIDELDRELITLLSRDARVSNRKVASELGVTEGTVRGRIK
                     RLQQDGLIAFTAITSFNTIDRSRLAFISVQADVQRVREIAREIADMPLINAVMITMGQ
                     FNILALCLFDELDYLIELASDRILAIAGVQHIETSIAVKTLKYNARMARITARAIQED
                     DEEAA"
     gene            complement(27496..27969)
                     /locus_tag="VFF98_17905"
     CDS             complement(27496..27969)
                     /locus_tag="VFF98_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZU65568.1"
                     /translation="MTQSIPLDAIDRKIIEHLRTHGRASNQQIAELLGLTATTVSSRI
                     RRMEEANQLRVVAVSDFSAHGFNCLMRVMVEVDGRPASQVAAALAEFPEVFATHLVTG
                     RYDIDMLVALHGLEDLPGLLLDKFSQVDGIRALVPSVVVDIVKYQFDVAPIEAKS"
     gene            complement(28126..28884)
                     /locus_tag="VFF98_17910"
     CDS             complement(28126..28884)
                     /locus_tag="VFF98_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65569.1"
                     /translation="MTDLGGKRALVTGAGQGVGLGIASALAAAGADVILAGRTLDKVE
                     RAAADITARGGSARALACNVRAAADIAAAMAQAQAWGGLDILVNNAQEVPNGQLSEVS
                     DEAFLGGFESGPLASFRLMKLARPQMAARGGGTIINLASSAGIRWDMSGYGAYGAVKQ
                     AIRALTRAAAAEWGRENIRVLAIAPHAESPGLKWWIANNPEEAEAFFRTIPLGRIGRL
                     EEDIGRAVVALCGADLSYLTGATIPLDGGQANFD"
     gene            complement(28906..29961)
                     /gene="dmpG"
                     /locus_tag="VFF98_17915"
     CDS             complement(28906..29961)
                     /gene="dmpG"
                     /locus_tag="VFF98_17915"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZU65570.1"
                     /translation="MMASATPPRFNADAGDRLYIQDVTLRDGMHAVRHMYGLDAVRAI
                     ARALDAAGVDAIEVAHGDGLSGASFNYGFGAHTDWEWLEAVAEVLERAVLTTLILPGV
                     GTVEELKRAHAIGVRSVRVATHCTEADVSRQHIGIARDLGMDVAGFLMMSHMIAPERL
                     AEQAALMESYGAHCVYVTDSGGALDMDGVTARLEAYDRALKPETQRGIHAHHNLSLGV
                     ANSIVAAQQGAMRIDASLAGMGAGAGNAPLEVFIAAANRKGWNHGCDVMALMDAAEDL
                     VRPLQDRPVRVDRETLTLGYAGIYSSFLRHAEKAAAEYGIDTREILVELGRRKMVGGQ
                     EDMITDVALDMLRQRAG"
     gene            complement(29958..30899)
                     /locus_tag="VFF98_17920"
     CDS             complement(29958..30899)
                     /locus_tag="VFF98_17920"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HZU65571.1"
                     /translation="MERVKAAIIGSGNIGTDLMMKMIKYPQNMELAAVVGIDAASEGL
                     ARAREHGIATTHEGLEGLKALPCYAEIGIVFDATSAYAHKLHDEALRADGKHVVDLTP
                     AAIGPFIVPPVNMDANPDATNVNMVTCGGQATIPMVAAVSRVARVHYAEIVASVSSRS
                     AGPGTRANIDEFTRTTARAIEAVGGAAKGKAIIILNPAEPPMIMRDTVFTLSEGASEE
                     EIRASVAAMVAEVQAYVPGYRLKQEVQFERFGDNNPLTIPGLGQFRGLKTMILLEVEG
                     AGDYLPPYSGNLDIMTAAAKATAERIAQRRLARQENA"
     gene            complement(30913..31722)
                     /locus_tag="VFF98_17925"
     CDS             complement(30913..31722)
                     /locus_tag="VFF98_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZU65572.1"
                     /translation="MSHAAASTARTAELGLALFAAMEARRTIAPISAADPSLTIEDAY
                     AISLAFLGHRRARGEKVVGKKIGVTSKAVQDMLGVHQPDFGFLTDRMAVQGRIDIAAH
                     GLIAPRAEAEIAFILKHSLNGPGITAADVLAATASIAPCFEIVDSRIDNWQIGIVDTV
                     ADNASCGVFVLGEERLDPAGLDLPGLHVAVSKNGAPLSEGFGHAVQGDPAAAVAWLAN
                     TLGAHGVTLDAGDIILSGSLVPLAPAAPGDHFEMVLSGRQGPIGRCAARFV"
     gene            complement(31719..32543)
                     /locus_tag="VFF98_17930"
     CDS             complement(31719..32543)
                     /locus_tag="VFF98_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZU65573.1"
                     /translation="MTTLTKTTHRLSGGHSIVLQEAGAGPAVVFIHGSGPGASGASNF
                     RQNIEAFVAAGYRVILPDLIGYGASSKPDGIDYTLQLFTDTLLEALDLHGIAEAILVG
                     NSLGGGIAIQAALDRPGLARALVLMAPGCLAPREAYFAMPGIAGMVSSFGGPDFNLAE
                     QQRLIANLLHPDAHGLITDALVAERFAVASSQPKEVLVRMRTPDLSPRLGEITAPVFV
                     LWGREDAFCPASHAALLLDACADVRAITFARTGHWVQVERAAEFNAYTLDFLKARA"
BASE COUNT         5054 a        10755 c        11585 g         5291 t
ORIGIN      
        1 gccgcctggt cacgcgaggt gatggccaat tacaagatcc cgcgccgctt cgtcgtcatg
       61 gacgagctgc cgcgcaaccc ggccggcaag gtgctgaaaa cggctctgcg cgactgagca
      121 ggcctcaggg ctgcacaaaa aaggccccgg tttgccgccg gggccttttt gcaggaaggg
      181 cgccgggtgt caggcctcgg ccagcgcggg ataatcggta tagccgcgcc ggtcgtcgcc
      241 ctcgccggtg tagaaatggg cgcgatccgc ctttgccagc ggcgcgccag cgcgaaaacg
      301 ggccaccaga tcggggttgg cgatgaacgg atagccaaag gagaccgctt ccgccccgcc
      361 actggccagc agcgcgccgg cccgctcgaa ggagagcgcg ttattctcga tcactgcgcc
      421 cggccaatgg gcgcgcacca gttccagcgc atcgaagccc tggttgggga tgtggatgag
      481 gtggacatag gccagcccca tgccggcaat cgcctgcagc agcgtggcat gggtttcggc
      541 cggatcggca tcggccatgc cgttgaaagt gacgccgggg cagatgcgca ggcccacccg
      601 gccgggcccg atcgccccgg ccatggccgc cagcacttcc accacgaagc gcacccggtt
      661 ggcggccgat ccgccatagg catccgagcg ctggttggtg ttcgacgaca ggaactgcat
      721 cggcaggtag ccgctggcgc aatgcagctc caccccgtcc atgccggcag cgcaagcgtt
      781 gcgagcggcc tgggcgaatt gcgcgatcag cgcggggatc tcgccggtgg cgatggcgcg
      841 cggcatctcg gggggcaccg gtacgccatc ggggccgggg atccggtcgg ggctggcgat
      901 ggcgctgggg gcgatggtat cggcatcggc ggccttgttc gcgcgcacgc tggcgcggcc
      961 gcaatgcatg atctgcatga cgatgcgccc gcctgcgcca tgcaccgcat cggccacctg
     1021 tgcccagccg gcgatctgtg cgggcgcatg gatgccgggc gtgcgccaat agcccttgcc
     1081 ggcgggcgag ggctgggtgc cttccgtgat gatcagcccg gcgctggcgc gctggcgata
     1141 gtattcggcc atgatcgggg ttggcacatc gccctcgccg gcgcggtcac gcgtcatcgg
     1201 cgccatgacg atgcggttgg caagggcgat atcgcccagc tgcacgggag agaagatatc
     1261 gctcatcggc tcagctcctc acgcgcgggc ggggcagggg ggcgccgcgc cgcatcgtct
     1321 cgatgaactt ctccagcggg gcgggcggat ccaccggccc gtcatgggcg gtgccgccgc
     1381 gctcccaata ggttttccag ctggggaaca ggtcgtggaa gctgccttca tagggtggca
     1441 cgcccggcca gcgcatcttg cgctcgccga tcggcgggcg gttgagatcg gcggcatacc
     1501 agtagagcgg caggcgccgg cggaaatgcc agcgcccccc gatccgctcg tatttgtcga
     1561 aatacatcat ctgcatgatc acccagtcgc cgccggcctc atgctcgttc ttggaataga
     1621 cgatgccctg ggcatggtcg gcatcgtcga aatcgatgat gtggccgccg atatggtggc
     1681 tggtgccggt gaactggcgc gaatgggtgt cgtcgaacca gtcgcgcagg gccttgcggc
     1741 cgtgctggcc gccgcccacc ttcacatcct cgggaaacag gttcacccag gcatcggcat
     1801 cgcgcatgtc gagcgcgagg gcatatttgg ccggcagctg gcggatcgca tcgagcgatt
     1861 ccagccggtc tatgcgtgcc agcagggcgc tggtgtcagt catctcgttt ttctcctctg
     1921 tgccgcctgc tcagaacgtc acgtgctcag aatttcacgc ccacgctcag cccataggtg
     1981 cgcggatcgg tgaaataggc caccgtgagc ccgccgaagc cggggccgaa atcgatgtaa
     2041 ttggccggtt ttgcgtagtt ggtgaggttc ttcacccagg ccgacacatc cgccttcaca
     2101 aagcccagcg ggatctgcat cacgctggcc cgcaggttga ccagcgtgcg cgcgggggcc
     2161 tcggaggtat aggcgctctg gtcgctgggg ctgctggtgg ccagcgcata gggataggtg
     2221 aaatagctgg atacatagct gagatcgccc atcaggtgca gctcgcccca atggccccgg
     2281 gccagccgcg catcggcgtt gagcgaggcg gtgtagtgcg gcgcatgggg gaaggcgcgg
     2341 ttgttcgaca catccacccc gccatcgatg aagctttcgt aatgggcatc gagataggcc
     2401 agcgagccgc tgatggtgag cgcgctggtg gggcgcagca cggtttcgaa ttccagcccg
     2461 cggatgcgcg ccttggccgc gttcagcacc gccgaggcgg ccgccccttg cgcggtgaag
     2521 accgacagct ggatgttctt gtgctcgtcc cagaagccgg cgacgttgaa gatcaccttg
     2581 ccgcccagcc atttggtctt ggcgccgatc tcgaaactgt cgaccgtctc gggcttgtag
     2641 gggttgcaca gttccggcgc gcccgagggg cactgggcgg tgggggtggt gaaggtgctg
     2701 gtctcgccgt tgaagccgcc cgatttgaag cccttggcat agcgggcata gaggttgata
     2761 tcacgcaagg gccggaaggc cagggtggcg gccggcgtga agctgtcgta tttggcgtcg
     2821 ggcacggcgc cataggggat gttggccaga acggtggggc tggtgatgcc gatcgacggg
     2881 tcgttcacca cttcgtagaa gcgggtgata tccttctgct cgtgggtgaa gcgcgcgccc
     2941 agcgtcagct tcaggcgctc ggtgaaggca taatcggcct gggtgtaggc agccagcgcc
     3001 cgcgtgtgcg agccatagtt tgaatcgaac accgcagagc cggcgaaata ggtctgcggg
     3061 ttataggtga aggccttctc gtagtagtag aacaggccgg cgacatagct gaggcgggca
     3121 tcgagcgcct tgccggtgag ctgcagttcc tggctgaagc tgcggaaata ggtgtcgcgc
     3181 acggtgaggg caacgggcag cggcgagcca tcgagatcga gcgaatcggt ccagtgcatg
     3241 ctgcgcgtgg cggtggtgga tttcagcgtg gccgcgccca gatccagcgc cacggtgagc
     3301 gcatggccat aggtgcgcga ccgttcatat tccggcgcat cgagcgcggc gaccgactgg
     3361 cgctgggtgt tgagatagcc ggccagcggg aaatagccgc cggcatagcc agaggcggcc
     3421 ggattggtga agatatcgcg cggatcgcca ttggcgttca ggtggtagag ctgggcgaat
     3481 tgcggctgct ggttgtattc ggtgtagtcg aacatgtaat cggcggtgag gcggctgccg
     3541 gccgggtgat agcgcagctg caccatgccg ctcttgccgt tcagcgtgtc ggtgtggccc
     3601 tggggcgaga gctgcacgaa gcccccgcgc gactggagct gccccgacag cttgagcgag
     3661 aaggcccccc aggcgggcag atcgaggatg acccgctcct tccagtagtt gtaattgccg
     3721 taggtgactt cggcccggcc ctccagcttg cccgagggcg cgcgcgggat gaggttgatg
     3781 gcgccggcca gcgcattgcg gccataaagc gtgccctggg gcccgcgcag cacttccacc
     3841 cgctccagat cggcaacgtc gaagatcgag ccttgcgcct tggcgatgta gacgccgttg
     3901 agatagaggc ccaccgccgg ttcccaggtg acggccgggt tgatcgtgac cgacccgcgg
     3961 atggcgatct gggtgatggt cttgtcgccg ggcgcgcgtt cgatggtgag gtcgggcgcc
     4021 atcgtgccca gcccctcgat cgaggtgatg ccgcggcttt gcaggaactg gctgccgacg
     4081 gcggtgatgg cgatcggcac ctgctggatg ttttccgccc gcttctgcgc ggtgacgacg
     4141 atctcgccga tgccggtctg gttggccgga tcggcggcgg gcgcggggct gtcggcaagg
     4201 gcggcctggg cgccggcaac aaggccggcg gacagggcgg tggtggcaag caggcgtttc
     4261 atgactatcc cctcgttgac gcgcatccct ctttggagat tgcgcagaat caatttgaga
     4321 tagttacgca aataaaagtc atatggcaat tgttgccgtc atatgcggtg ctttatgcgg
     4381 tgtttcatgc attgtctgca tgtggggcag gggccggcgc ggcttgccac cagccgatgc
     4441 cggccgcatc gggcgcgcaa gtggcaaggc cgcgtgtgcc caggcaggcg agatgggcga
     4501 ggcattcgcc ggtggccatc tgcagcgtgc gcgggccgat cttctggcgg aacagcgcgc
     4561 caaacacatc gaccgcgcgc tgcggctctg tcagccgggc gtgcagccgg gccaggccgc
     4621 gctcgtgccc gtcgatcagc gcgtcgatgc gcgcgtgcag gccgcgaaag gggcggccat
     4681 gggcgggcag caccagcgtg tcatcgggca cgcggcggcg gatggcggcc agcgaggcca
     4741 gccagtcgct cagcgggtcg gccgcaggtt cctgcggata gacagagacg ttggaggaga
     4801 tatcgggcag cacctgatcg cccgagatca gcaggccatc ggcggcgcaa tgcagcagcg
     4861 catgctgggg cgaatggccc tgccccatca gcacttccca gctgcgcgcg ccgatcgtca
     4921 ggcgctggcc ttcgcgcagg gcggcaaact ggctgggcag cggcgccacg atctggccaa
     4981 aggcgcccag cccggccagc acctcgtcca gttcggcctc gctccagccg gcggcgcggt
     5041 agaaggcgcg cagttcgggc ggttcgccat ggcccagttc gggcaccagc gcgcgcagca
     5101 tgtaatattc cagcgggctc atcgccagct gtgtgccgtg gcggctggcc agccagccgg
     5161 ccatgccggc atgatcgggg tgctggtggg tgacgatggc gcgcggcacg cctgcggcca
     5221 gcgggccttc catcgccctg cgccaggcct gcacggtgtc gggcgtgtgg agcccggtat
     5281 cgaccaccgt ccagccatcg ccatcggcca gcgcccagac gttgatcgcc gccagcgagc
     5341 cggcgatcgg catgcgcagc cagctgagcc cgggcatgac agcagcggcg gaaccatcgc
     5401 cgatgaccgg aggttcgatg gccggatagg taagcatggc agggcctttc gacaggggaa
     5461 aatcaggtgt gcgaggcagg gccggcaagg gcggcaaggg cggcaaggat ttcgccattg
     5521 tgctggccga tgtgtggcgg gggcagctgc acggcaaagg cgaaatcgct catcctgatc
     5581 ggcagggcat attgcgtggt ggtggcgccg ccgccagcca cctcgatcac catcgcgcgg
     5641 gcctgcgcct gcgggtgggc cagcgcttcg gccaggctgg gcacgggcgt ggcgcaggca
     5701 tcgatatcgg ccaggatcgc ctgccattcg gccagggtct tgctggcgat ggtggcggcg
     5761 atctcggcca cggtggcggc cagctgctcg cccatgtccc agccgcgcgg ctccagatcg
     5821 ggccggccga tcgcctggca gaacgcgcgc cagaacttgt gttccagcgc ccccaccgcc
     5881 agatggcgcc cgtcggccgt ggcataggtg cgatagcagg gcaggccgcc gctgaggagg
     5941 tcgcagcccg gcaccggcgc ctggccgccg cgcagcatct gcccggccag cggcatcagg
     6001 ttgagcgcca tggtgcaatc ggccatggcg atatcgatat ggcggccgat gccgctgcgc
     6061 tgggcatcga acagcgcggc cagcatggcc accgccgccg acaggccgcc gccggccata
     6121 tcggccagcg gcgtgctgcc cggcgatggc cggctgccat cgacgacatt ctggccaagc
     6181 acgccggcca ctgcctgata gttgatgtcg tggccggccc tggccgccag tggcccgctc
     6241 tggccatagc cggtgagcga gcaatagacg agccgcgggt tgatcgcccg cagcgtgggg
     6301 taatccaggc ccagccgcgc catcaccccc ggccggaagc tttccagcac ggcatcggcg
     6361 ctttccacca gctggtgcag ggtggcgatg gcggcggcct ccttgagatc gagcgcgagg
     6421 gatttcttgt tgcggttggt gaggtggaac aggccgcctg gcggcgtgcc ggccgggccc
     6481 cgggcagcct cgccatgcac gggcggctcg atcttgatca cctcggcgcc cagatcggcc
     6541 agccacaggc tgcacacagc gcccggcagc agcgcgctga aatcgaggat gcgcagatcg
     6601 gcaaggggtg gtttcgacat gggctcagcc ctccagctgg cggaaaggaa catcgcggtc
     6661 ggtgcggatc tcgccgggca ggcccagcac gcgctcggcg atggtgtttt tctggatctc
     6721 gtcggtgccg ccggcgatgc gcgcgcccgg cgctgccagc cacaggcgct gcggatgggc
     6781 ccagcggggc cccagatcgg ccgcggcgat cagcccttcg gcgccgccca gctcgatggc
     6841 cagcgcgccg ccggtctgga aggcgcgggc gcccaccagc ttgatcgccg ccatctccgg
     6901 ccccgggctc tgcccgcgcg agatggcgct ggccccgcgc cggacgatca gctccacgcc
     6961 gaactggctg agccaggtgt gggcgatgcc ggcgcgcacg cgggcatcct cgatggcggg
     7021 gcggccgccc cattgcgcgg tgcgggccag gccgatcagc tcggcatgca gatcgccggc
     7081 gaaattccgc cccagcgaga agcgctcctg catcagcgtg ccgagcatca ctttccagcc
     7141 ttccccttcc gggcccagcc ggtcgctgtc ggggatccac acgtcggtca ggaagacctc
     7201 gttgaaatgg gcctcgtcgc tggcctggcg gatcgggcgc acctcgaccc cgggcgcgcg
     7261 catgtcgagg aggaacatgg tgaggcccct ggtcttgggc cggtcggggt ctgtgcgggc
     7321 gagcagcacg ccgaaatcgg cgaaatgggc gcccgatgtc cacactttct ggccgctgag
     7381 gatccagcca tcgccctcgc gccgggcccg catgcgcagg ttgccgatat cggagccggc
     7441 ggcgggctcg gaaaagagct ggcaccagat ctgttcgccg cgcagcgccg gggcgatatg
     7501 gcgctgcttt tgcgccggcg tgccccacat ggcaacggtg ggcagcgccg tgcccaggct
     7561 gacctcgaag acatggccgg gcacgaggaa gcgggcttcc tcctcatcgt agatcgcctg
     7621 ctgggccggg gtgccgccgc gcccgcccat ggcctgcggc caggtgatgg cggcatagcc
     7681 ggcggccgcc ttgccggcct gccaggccct ggcggcggcc agataggcgg ccggatcaag
     7741 gccggcgcca aaggcgtcct cgcggctgcg gcgggggtgg gcatgggtgt ccagccaggc
     7801 gcgcacctgc ctgcggaagg cggcttcggc gggggtgtcg ttgaaatcca tcaggcggcc
     7861 tcctcttgcc cggcctcggc gatcagggcg gcggcgaggc gttcttccca atagtggcgc
     7921 gtgcccagaa tctgccccag ccagcgcgcg cggcgcaggt gcaggtggca atccatctcc
     7981 caggtgaagc cgatgccgcc atgcaggtgc aggctttcct cggccgccag cgtgtaggca
     8041 tcggtggccg ccacccgggc agcggcggcc gcgcggggca gttcgggcgc gtttgtggcc
     8101 agcgcccagg cgccgtaata ggcatgggcg cgggccagtt ccacggcgat gtagatatcc
     8161 gccagcttgt ggcgcaccgc ctgaaagccg ccgatcaccc ggccaaaggc gcgccgttcc
     8221 agcgcatggt cacgcgcggc atagagcgcc ttttccgcgc cccccacctg ctcgaaggcc
     8281 agcagcacgg cggcgccatc gcttatcctg gcggcgatgg cgcggccacc ggcagggcac
     8341 agcacctgcg ctggcgcggc ggtgcaggcg atctgcgcca gcggccttgt gggatcgagc
     8401 gaggcgcgcg ggcagcgctg caccccgggc ccggcaaggt cgaccagcac cagcgcatcg
     8461 ccggccagca cgatgattgc ctcggccagc atgccatcgg ccaccgcgtc cagctgcgcg
     8521 gtcagcgtgt gggcggccag caggggggcg ggcgtgggcc ctgcgtgctg gccgtgctgg
     8581 tcgatcgcgg cggtgaggat ggccgtgccg gccgcgatgc ggggcagata ttgcgcttgc
     8641 agggcagggc tggcggcatg aagcagcagt tcggcggcga gataggtcgt gcccagcagc
     8701 ggcaccgcgg ccagcgcccg gccggcctcc tcggccagca ggcacagttc cagccggccc
     8761 atcccggcgc cgcccagcgc ttcgggcagg gccaggccca gcgcgccgat atcggccagc
     8821 gcgcgccagc tggcggcgct gtggcaggcg gtgccctcca gcacccggcg cacttcggcc
     8881 ggggtgctga tagcgcccag catgcgccgc agttcgtcct gcaggcggcg ctggtcttcg
     8941 gaaaagtcaa aatgcatggc agcctctcct gtcaggcggc gcggcgggcg ccgggcgagg
     9001 gagcggcccg gtcgccatgg aaggtctgcc cggccgcggc catggcgcgc agcagatcgg
     9061 gcagggcata aagagcgcca tgggcggcgc acagccggtc gcattcggcg atgacggcgg
     9121 ccaggcccag cgtgtccatc gccgcgaaag gcccgcccag ccacgagggg aagcccaccc
     9181 ccagcgtggc ccccagatcg ccatcggtgg catcgaccag cacgccttcg gcaaagcagc
     9241 gcgcgccctc ggccagctgg gcatgcagga ggcgcgcctt cacctcgccg atcgccggct
     9301 ggcgggccag ctgcggcagc agcgcgccca gccccggcca caggcgcttg ccgccatcgg
     9361 cttcgtaatc gaagaagccc atgccgttct ttgccccatg gcgcccggct tggtagagcg
     9421 tgccgatgat gccggcatgg tgggcggtgc cgcgctcctt cgcctgggtg gcgccggcgt
     9481 gatagctgac atcgaggccg atcgtgtcgg agatggtgag cgcgcccatc ggcatgccca
     9541 gcatgcgcgc gccattctcg atcagcgccg gtttcacccc ttcctccagc atggccaggc
     9601 tggcgctgac gaaggcgccg atgaagcggg tggtgaagaa gccgggcccg tcgttgacgg
     9661 tgatcggcgt cttgcgcagc cggccgacga aatcgagcgc atgggcccag caggtggcat
     9721 cggtctgcgc gccgcggatg atctcgacca gccccatgcg ttccaccggc gagaagaaat
     9781 gcaggccgat gaagcgttcg ggccggccgg cgctggctgc caggcccgag atcggcaggc
     9841 ccgaggtgtt ggaggcgata agggcggtat cggccacggc ggcggcgatg cgggcgatca
     9901 ccgcctcttt caccgcccgg ttttcaaaga ccgattcgat caccagcgtg gcttcggcca
     9961 gatcggcata atcggtggtg gggcagatcc gcgccaggct ggccgctgcc ctggcgggcg
    10021 tgccgcggcc tttctcgatg gcgcgttgca ggcgcttttc ggcataggcc ttgcccttgc
    10081 cggcgctggc catgtcgcgg tcgagcagca ccacctcgat gccggcctgg gccgcgcaca
    10141 gggcaatgcc ggcgcccatg gtgcccgcgc ccaccacgcc gatgcgcgcg cagggcagcg
    10201 cggcaatgcc ggccgggcgg gcagccagcg catcggcccg gcccttgttg acgaacagcg
    10261 tgcggatgag attgccggcg acagggttca tcatcaactg ggcaagatat ttggcttcca
    10321 cccccagcgc cttgtcgatc ggcagctgca gcccttcata cagcgcggcc agcagctttt
    10381 cgggagcggg ataattgcca aagcccttgg ccctggtcag cgcgttggcg ccgatgaaca
    10441 ggttgcggaa gccggcatcc gtgctgccgg cgccgccggg cacgcaaaag cccttgcggt
    10501 cccatggctg cacggcatcg ccggtttcgc gcagccaggc cttggccgtg gcgatcagcc
    10561 gggcggcggg caccacggca tcgaccaggc ccatcgccag cgcttctgcc gggcccacag
    10621 agcggccctc catcatcatt ggcagggctt tggccagccc caccagccgg ggcaggcgct
    10681 ggctgccgcc gccgcccggg atcagcccca ccatgctttc gggcaagccc agctggatgc
    10741 gcggatcatc ggtcaccacg cgatggtggc aggccatggc gatctccagc ccgccgccca
    10801 gcgccagccc gcccacagcg caggccaccg gcttgccggc ggtttccatc cagcgcagca
    10861 ggcgcgacag gtaggagaac aggtggacga tctcttccgg gcgcttgccg gccagatcca
    10921 cgcccttgcc gatctgcttc agatcgccgc cggccacgaa gctgtccttg cccgaggcca
    10981 gcacgatgcc tttcaccgcc ggatcggcca tggccgcgcg cagcgccgct tccagcgggt
    11041 cggtcagcgc cgggcccagc gtgttcatct tttcgcccgg cagatccatg cagatggtgc
    11101 agatgccatc ggcatcgatg ctggtggtga tggcctggtt catctcttgc tctcccctca
    11161 ctgcacgcgc tccagcacca tggcgatgcc catgccgccg gccgcgcaca gcgaaaccag
    11221 ccccaggcca agcccgcgcc gctccatctc gtcgatcagc gtgccggcga tcatcgcccc
    11281 cgatgcgccc agcggatggc ccatggcgat ggcgccgccg ttcacattca tgcggtcatg
    11341 cgggatgttc atcgtctgca tcagccgcag cggcacggcg gcgaaggcct cgttcagctc
    11401 ccacagctcg atatcggcag gcgccaggcc agcgatcgcc agcgcgcggg cgcaggccgg
    11461 cgttgggcca tcgagcatca gcgccggctc cgagccgacc gagacgaaag cgcggatgcg
    11521 cgcgcgcggg cgcaggccca gccgcgcgcc cgcctctgcc gagccaacca gcatcagccc
    11581 tgcgccatca acgatgcccg aactggaagc ggccgtgtgc acatgctcga tgcggtccac
    11641 ctcgggatag cgctggatcg ccagcgcatc gaagcccatc gagcggccca gcccggcaaa
    11701 ggccggcttc agcgcggcca gggtggccgg cgttgcatcg gcgcggatgt gttcgtcctg
    11761 tgccagcagc acggtgccca tcatgtcgcg caccggcagc accgagcggg caaagcgccc
    11821 ctctgcccag gcctgcgccg cgcgctggtg gctggccagc gcataggcat cgagatcagc
    11881 ccggccatag ccgttgagcg ttgccagcag atcggcgctg atgccctgca tgacaaaggg
    11941 cgtgtggaag ctgacgcgcg gatcgatgcc atgcggcccg ccgccatcgg cccccatcgg
    12001 aatgcgcgac atcgcctcca ccccgccggc gatggccaga tccgccatgc cgcagccgat
    12061 cttggcggcc accaggctca ccgcctccag cgccgaggcg caggcgcggt tgatctgcag
    12121 gcccggcgtg ctgtcggcaa agccggccag cagcggggcg atgcgggcga tggtggcgcc
    12181 ctgttcgccc gccgcgatga ccgtgcccag caccacttcg tcgagcgcgt cgtcgggcag
    12241 ggtgttgcgg cttttcagcg cggccagcag ctggcgcacc agctccacag gggtggcctc
    12301 atgcagcgcg ccatccggct tgccgcggcc gcgcggggtg cgcaggtgat cgtagatgaa
    12361 ggtgtctcgc atggctctcc cgacctttgc gatggatatt tatataatgc attataagtt
    12421 tgtctgcgca ggtcaagcga aatgccgccc cgccgcgcgg gcggaatggg cgttgcaagg
    12481 tttttcagca ggttgccggc tattgcggcg gggcgagcac cggtgccgcg gcacgaatcg
    12541 tcgcttccag aggggccctg cagggggtat ccagcaagcc caggaacacc agcgcgaagc
    12601 tggcctgcaa gcctgcgcgc agctggtcga tatcccagcc ctgcagcgcc cattgctcga
    12661 aattggcctc gaacaggcgg atcagcgtga tctctaccag cgccggcggc acatcggcga
    12721 tcagcgcccc cgaggcggcc aggctgtgca ccagcggcgc cagcagatgg ccgccgcccg
    12781 gcacccccat ctggtggtgc tcgccgggcg agagcgtgtg gaaggccttc cacagattgc
    12841 ggaagaagcg cggctcgcgc gcataggcgg ccaccagccg atcgaccacg ccgaggatgc
    12901 gggccacagg atcggcttcc tccatccagc cattgtcgcg gtgaaagccg cgcacctgcc
    12961 gggcaaacac ctcgcccagc accgccgcct tggagccgaa cagattgtag ggcgtggcca
    13021 ggctgagccc ggcgcgcgcg gccagttccg gcatggaaaa ggcgccctcg tcgcgctcgg
    13081 cgatcagctc gctcgcggct ttcaccagcc gccgccgtcg ctcggcgatg gcctgggcgc
    13141 gcttgccctg cggcggggcg cctggggcgc cggtggtgtc gctggcattc tgggtcaagc
    13201 cgggcccctc tgcttgtgcc gtttgcgcag gctgctagca ttgccgggcc accgctggct
    13261 agagccgccc gccgcctgtg gcccgggcgg gatcagagga aatagctgcc gccattggcc
    13321 gccagcaccg aaccggtgac aaagctggca tccgcgctgg cgagaaaggc cacggccgcg
    13381 gcgatctcgg ccggatcggc catgcgcccc agcgggatcg cctgctgcat cgcggtgatc
    13441 cattcggccg gaatgccctg catcaccggc gtgtcggtgg ggccgggggc cacggcgttg
    13501 acccggatgc cgcgcggcgc caactcgcgc gccagttgcc gcaccaggcc gatgatcccc
    13561 gccttggagg cgcaataatg ggccgaaccc tcgcccgatt gcgcgctggt ggagctgagc
    13621 gcaatgaagc tgccgccgtg cccgtcttgc gcaaggaggc gggtgaaggc ccgggccagg
    13681 cggaaggcgc catcgaggtt gaccgccatc accgccgccc agccctcgtc ctccatgtgg
    13741 atcagctggt cgacgatggt gtcggatcgg ccatgctcgg cgatttcggc atggcgccgg
    13801 gccattgccg catagaacgc gtccgatcca tcctgcgcgg cgcggccgat gccggcattg
    13861 tgcacggcga tgtcgatccg gccgaacgct gtgcgggcct cggccagcgc ggcggcgacc
    13921 gaggccgagg aggtcacgtc gcaggccagc gccagcgccg cgccgctatc ggccgccgcg
    13981 cccggctgca gatcgagcgc ggccacctgt gcgccctcgg ccgccagccg ccgcacgatc
    14041 gcctggccaa tgccccgccc ggcgccggtg acgaccgcgc cctgcccctg aagccgcccg
    14101 cccatcacga gggcatgtag agctgggccg ccttgccgcc atcgacgggc agcgcggtgc
    14161 cggtgatgta gctggccgca tccgacagca tgaaggcgat cgcctcggcc agttcttccg
    14221 gctggccgcc gcggcccatg gggatggcct ctgccgtgcg ggtggcgatt tcgggcgcct
    14281 tggcggcaaa atcctcggtg ccggcggttt gcacctggcc gggcacgatg gcgttgacgc
    14341 ggatgccctg gcgcgcccct tccatcgccg ccaccgcggt gaactggatc agcgccgcct
    14401 tggaggcgga atagctcgac atgaagggcg ctgcgcgaat gccgcaggtg gaggcgatgt
    14461 tgacgatggc gcccttgccc tgcccggcca tgatggccat cgccgccttg gtggccacga
    14521 aaacggcatc ggcattcacc gcgaaatcct tgcgccagtg gctcagttcc agccggccga
    14581 tcggcgcgta atgggtggac atggcgttgt tgaccagcat gtcgagccgg ccgtggcggc
    14641 tggcgacatc ggcgatcatg gccgccagcg cgtcgaaatc ggtgacatcg aggcggatcg
    14701 cctccaccga gccgccctcg gcgccgatct gcgcggcggc cttgtcgagc agttcctggc
    14761 ggcggccgca gatggccaca tgggcgccgc gccgggcgag cagggcggcg gtggcaaggc
    14821 cgatgccatc gctgccgccg gtgatgatgg ccacctggcc ggcaagcggg cgcggggtgg
    14881 gggtgtgatc tgccatgaaa ggtacctctt ccgggggatg atactggttt gctgcaagcg
    14941 caaatggatg ggcgggcagc agacgaatta tccaatgcgt atttttttac gatatgatct
    15001 acgaataatg tggatcgcgc cggatgcaag agtgatttgc ggggcagcgc gccccgccac
    15061 cggccgggct gccaccggcc ggatcggccc atgcggcgat tttcgcgttc gcgcggcggg
    15121 gcggcttgct cattcaatca tgcgtaaaaa aattatcttt caaatccgca aatcatcgaa
    15181 taccgttctg ccagacggag taacgcgtcg aatcatggga gtggatattc gtgcacaagg
    15241 tcgcacttgt aaccggggcc agccgtggcg cagggcgcgg cattgcccgc gggctgggag
    15301 agctgggcta tacggtctat gtcaccgggc gcacgctcag cccgggcgat gcccggggct
    15361 gggacgggtc tgtcctgccc ggaacggtga gcgaaaccgc cgccgaggta accgccgcgg
    15421 gcggcagggg cattgccgtt gtgtgcgatc atgccgatga tgcccaggtg gccgccctgt
    15481 tcgcccggat cgcggcagac gagggccggc tcgatgtgct ggtcaacaat gccacctata
    15541 tccaccccca gctgatcgag aagatgccct tctgggagaa ggatctcgat gcggtgaaga
    15601 tcctcgaggt ggggctgcgc tcggcctatg tggccagctg gcatgcggcg cgcatcatgg
    15661 tgccgcaggg cagcggggtg atcgctttcg gctcctcctt tggcggcagc tgctacatgc
    15721 atggcccggc ctatggcgcg cagaaggccg gggtggacaa gctggcccat gacatggagc
    15781 atgatctgcg cggcaccggc gtggtggccg tgtcgatctg gatggggccg ctggtgacag
    15841 agcgctcgcg catcgccgcc cagacccacc cggagcagta tgaaggcttc atcgaaaccg
    15901 ccgagaaccc cgagttcacc gggcatatca tcgatgccat cgcccgcgcg cccgagcgcg
    15961 acacgctgtc gggccagacg ctgatcgccg ccgagatcgc ccgccagctg ggcgtgaccg
    16021 accgcggcca ggagcgcccc tcctaccgcg agatgctggg cgccccgcgc cccaagaacc
    16081 cggcggcggt ttactgagat ggccggcagc ttccatctgg ccgacctgtt cgagaccgtg
    16141 gcgcgcaccg tgcccgggcg catcgccatc gagggcgata cgcgctgcct cacctatgcc
    16201 gggctggatg agcgggccac gcggcttgcc acggggctgg ccgcgcaggg gctggggcgg
    16261 ggcgatacgg tgggcctcta tctcaccaac cgggtcgagt tcatcgaggc cttcctggcg
    16321 gtgatcaagc tgggggccat cccgttcaac gtcaactatc gctaccgggc ggaggagctg
    16381 cgctatctct ttgccaacgg gcgggcggca gcgatcatcc atgatgcgca atatggcgag
    16441 atcctgcgcg gcctgcgcgg cgatctggcc gatctgaagc tgtgcgtggc ggtggcggat
    16501 ggttcgctcg aggcggcggc aggcagcctt gcctatgccg acctgctgga gagcgcgccc
    16561 gccggcccct ggcctcgcgc cgaggacgac atcatgctga cctataccgg cggcaccacc
    16621 ggcatgccca agggggtgat gtggccgcac cgggcgttct tctttgcctg cgcgggcggc
    16681 gggggccatt tcaatccgca tgggccggcc acctgcccgc aggatatcgc cgaccgggcc
    16741 gcccatggct atccactgcg gatgttcacc gtggcgccgc tgatgcatgc ggcggcgctg
    16801 tggacggtgt ggtcggcgct gttgaacggg ctgacggtgg tgctcgatga aagcgggcgc
    16861 ttcgatgccg cggccatctg gcagaaggtg gccgaccgga acgtgcagct gctgcagatc
    16921 gtcggcgatg cgatggcgat accgctgcgc gatgcgctgc gcgcccatcc ggggcggtgg
    16981 gatctgtcgg ggctggtcag cctgggttcg ggcggggcgc tgttctcgcg ccatgtgaag
    17041 gaggatctgg ccgggttgct gccgcctgcc gtgcggctca ccgatggcat gggctccacc
    17101 gagacaggcg ttgccggcat ggccgcggcc agcgccgagg gcatgatgca gctggaagcg
    17161 ggcgagacgc tgaaggtggt ggtggacgat cgcttcgccg tggccggcga gaccggcatc
    17221 atcgcccgtt cgggccacac gccggtgggc tatttcggcg atgcggagaa gaccgcagag
    17281 gtgttccgcc agatcgacgg gcggctgtgg gtcatcagcg gcgatagtgg ccggctggag
    17341 gcagatggcc ggatcaccgt tttcgggcgg ggttcgacct gtatcaacag tggcggggaa
    17401 aagatcttcc ccgaggaagt ggaggaagcc ttgcgcgctc atccggcgat cagggatgcg
    17461 gtggtggccg cccaggccga tgcccgctgg ggcgaaaggg tggtgggcat tgtctcgctg
    17521 cgggcgggcc atgcggcgcc ggcgctggag gaaatgcgcg gctttctggg cgatgcgctg
    17581 gccggctaca agctgccgcg ggcgctggtg tgggtggatg aggtgcaccg ctcgccggcc
    17641 ggcaagcagg attatcgctg ggcccgcgcc atggcggaga ccgcccgatg agcgcccatg
    17701 acgccgcctt tgccgcgctg accgcgcccg atgccccctt cgccattgtc gagcgcgacg
    17761 ggatgcgcca gttcgcccat gcgcccgccg atctggaagc gatgatcgag ggcgcccgcc
    17821 gccatggcga caagaccttc atcgtcgagg aagccggcga tggcagcctg cgccgcctca
    17881 cctttgccga ggtgtttgcc tggcgcgacg agctggccgc catgctgcac atccaccgcg
    17941 gcgagcgggt ggcgatctgc atgcgcaacc gtgccgaatg gatcgtggcc tttctggcgg
    18001 tggtgaaggc aggcggcatt gccgccctgc tcaacagccg cggatcgccc gccgaactgg
    18061 tggcgatggt gcatgaggtg acgcccgagc tggtgctggc cgatacagag cgcgccaccc
    18121 tgctgcgcgc cggcggctac accggccgcc tgttcgacct cacccgcccg ctggcgcccg
    18181 aagagatcgc ccgccgcagc gccgtgccgg ccgaactgcc cgcccctgcc agcggcgagg
    18241 atgcctgcgc catcctgttc acttcgggca ccaccgggcg ggtgaagggg gcggtgctgt
    18301 cgcatcgcaa cctgatcacc gggctgatgg gggtgcagct gtcgggcatg caggtgctgc
    18361 acaccatggc cgcggccatg ggccagccga tggaagcgct gctggcgcgc atgccgcagc
    18421 aggcggtgct gctggtctat ccgctgttcc atatctcggg gctgggttcg gcctttctgt
    18481 cgccgctgct ggcgggatcg aaggtggtga tcatgcgccg ctgggatgcc gatgtggcgc
    18541 tgcgcctcat ccgcgaggag cagatcaccc tgttcaccgg cgtgcccacc atgctgtggg
    18601 atgtgctgca caaggcgggc ggcggggccg gggatctggc ctcggtgcgc aatgtggcca
    18661 gcggcggcca ggcgctgccg gtgaacctgc tggcgcaaat gcgcgcggcc tgcccgcagg
    18721 cggtgatggg caccggctat ggcatgaccg aatgcgccgg cgccatcgcc caggccgtgg
    18781 gcgaggaatt cgtgcgcaag ccggcttctg ccgggcgggt gttgccgctg gtggaagcgc
    18841 gcatcgtctc gccgcagggc gcggtgctgc cggccggcga ggcgggcgag atccttgtgc
    18901 gcggggcgca ggtgatgcgc ggctactgga accgcccgga agacaccgcc gccgtgctgg
    18961 cgcccgatgg ctggctgcac accggcgatg tcgggatgat cgacgaggag ggctatatct
    19021 tcatcgtcga ccgcaagaag gacatggtga tctcgggcgg cgagaacatc tattgcgccg
    19081 aggtggagcg ggtgctgggc gaaatgccgc aagtgaccga atgcgccgct tttggcattc
    19141 ccgatgaacg gctgggcgaa ttgctggtgg cggtggtgcg gcccggtgcc gacgggcgcg
    19201 gccccgatgc ggctgcggtg atcgcctggg tgggcgaacg gctggcccgc tacaaggcgc
    19261 cggcccatgt tgcctttgtg accgaaacgc tgccgcgcaa ccatgtgggc aaggtcgaca
    19321 agatcgccct gcgcgcggcc tggccaaccc tttacggaga gaaatgacca tgggcatgcc
    19381 cgacaatatc cgcgtgatcg actgcatgat gggccttccc gaggcggagg accgggccga
    19441 gtggtttgcg cccttccgcc cgctcatcaa ggatgcgcaa acgctccagc agtttgccat
    19501 gcccgcgcaa tacatgttca aggacatccc ccagtcgggc cagcaggatg attacatccg
    19561 ctggacggtg gagcagatgg accgcttcaa cgtcgagcgc gcgctggtgg gctggaacga
    19621 caatgccacc tcgcgccgcg ccagggaact ctatggcgat cgcttcttct tcgatctgcc
    19681 ctgcgatccc aatcgcggca tggaggaagt gcgccgcatc aagcgcatcc atgccgaggt
    19741 gggccttgcg gcgatcagcg tgtttccggc cggcaccctg ccgcaggtgg cgatcaacca
    19801 caaatacatg ttcccgcttt atgcctgcgc cgaggaactg gggctgccgg tgctgctcaa
    19861 cgtgggcatt ccggggccgc gcatcccgat ggaaacccag aaggtggaac atctcgacga
    19921 ggtgtgctgg ttcttccccg atctcaaggt ggtgatgcgc catggcgccg agccgtggga
    19981 agcgctggcg gtgaagctga tgctgaaatg gccgaacctc tattattcga ccagcgcctt
    20041 tgcccccaag cattatccca aggcgatcat cgactacgcc aacacccgcg gcgccgacaa
    20101 gatcatctac gccggctatt tccccatggg cctcagcctc gatcgcatct ttgccgacat
    20161 gcagcatgtg ccgctgaagg acgaggtgtg gcccaagttc ctgcgcgaga acgcccggcg
    20221 cgtgttcaac ctcgcctgac ccccagcgca tagcggagac agaccatgag caacagcgat
    20281 accgccgcca ccgatgcccg cacgccgcct gtggcggtgt ggcagctgct ggaagggctg
    20341 aaggggcagg atcttgccga ctggcgcctg gcgccggtgg atggccgcgc ctcggtggca
    20401 gagcgcaacc ttgatatcgg cttccccttc ggctggtatc cgatcgagct gtcggcgcag
    20461 ctggccccgg gcgaggtgaa gccgctgcgc tatttcgcgc gcgatctggt gatgtggcgc
    20521 ggcgaggatg gccaggcgcg catcatggat gcctggtgca agcatctggg cgcgcatatg
    20581 ggccatggcg gcaaggtgca tggcaatctg ctggaatgcc ccttccacgc ctggcgctat
    20641 gatggcgacg agggggtcgt caaggagatc ccctattcgc gggcgatccc cccgcaggtg
    20701 aagcgccaat gcacccgcac ctatgcggtg acggaagcca accgctggat ctggatgtgg
    20761 tatcacccgg agggcatcgc gccgctgttc gatgtggcgg tgatccccga atgcgcagac
    20821 cctgaatgga ccgatttcga ggtgcatgag tggaacgtgt ggggcaatgt gcagaacatg
    20881 gcggaaaatg gcgtggatgt ggcgcatttc cgctatatcc acggcaccgc caacgtgccg
    20941 ctgggcgatc tgcgctgggg cgaatggggc cgcggcgccg atgtgaaggc caagatgggc
    21001 acgccctggg gcgaggtgga tggctgcatc agctatgaca cgatgggccc ggggcagagc
    21061 tggacgcgct ttaccggcat ttcggaaacg ctgctgatgg cctgcatcac cccggtggag
    21121 ctggaccatg cccacacccg cttctgcttc acccagccgc gcgcccaggc cgagggcgag
    21181 cgggccggca tcgcccgcgc catcatccgc gatatctgca agcagtatga ccaggacaag
    21241 gtggtgtggg accggcagaa gttcgagccc aacccgatca tctgcgaggg cgacggtccg
    21301 atcgcccagt tccgcaaatt ctacgcccgc tattatgtcg agggcgaggg gcgcccgccc
    21361 gtgcgggccc agggctgagg cgatggcagg cgccagcccc ctcgacacgc aggatgcgct
    21421 ggggctggcc gggcttgtcc ggcgcggcga agtggccccg ctggaactgc tggaagaagc
    21481 gatcgggcgc gccgaggcgc tcgatccgca gttccatttc ctcgcccagc gccattatga
    21541 ttatggccgc gcggcgattg cccgcggcct gccgcagggg cccttcaccg gcgtgccctg
    21601 gctgctgaag gacctgaaca cccatatcgc cggcctgccg agcgaagggg gcagtcgctt
    21661 ctatcaggga cagcttgcgg ccgagacatc cgagctggtg cgccgcatcg agcgggccgg
    21721 cttcgtcgtc ttcggcaaga ccaccacgcc cgaattcggc ctttccggca ccaccgagaa
    21781 ccgcctcacc ggcgccacgc gcaatccctg gaacccggcc catatcgccg gcggttcctc
    21841 gggcggggcg gcggtggcgg tggcagcggg cgtgctgccg gccgcccatg ccacagatgg
    21901 cggtggctcg atccgcattc cggcggcctg ctgcggcctg ttcggcctga agcccagccg
    21961 tgggcgcgtg ccgatgggcc cgccgcgcag cgagggctgg ggcgggctgg ccacccacca
    22021 tgcgatcacc cgcagcgtgc gcgacagcgc ggcgatcctc gatgccacct ggggggcaga
    22081 gccgggcgcg cgctatgctg cgcccacgcc cgagcggccc ttcctggccg aagtgacccg
    22141 gcccccccgc gccttgcgca tcgccctgat gaccgcccct ttcgccggat cgccggtggc
    22201 ggcgccatgc gtggcggcaa ccagggccgc ggcggccctg tgcgagcgcc tgggccacca
    22261 tgtcgaggaa gccgcgccgg cgatcgacgg ggcggcgctg ggggcggcct cttttgccat
    22321 tctgggcggc tcgatcgccg ccgatgtggc cgaccgggcg gcagccaccg gcctcgatcc
    22381 ggccgccttg ctggagccga tcacgctggc cttcatcgcc tatggcaagg gcttttcggc
    22441 catggattat gcccgcgcca atgccacttt ccaggccgcc gcgctgacca tggcgcgttt
    22501 cctcgaaacc tacgacgtga tcctcagccc cacgctggcc agcccgccgc tggcgctggg
    22561 ggagatcagc ctgtcgccgg gtgtgggcat ggatgtgtgg gggcagcgcg cgggcgcctt
    22621 tgccgccttc accggcattg ccaacatgac cgggcagccg gcgatgtcct tgccgctggc
    22681 gatgtcggac ggggggctgc cgatcggcgt gcaggtgttg ggccgctatg gcgaggaagg
    22741 gctgctgctg cgcctggccg gccagatcga gcaggccgcg ccctgggtcc tgcggcgccc
    22801 gccggcggcg gcttgagcga aaggatgggg gtgctggcag gcgctctggg gttgttgggc
    22861 ctgtttggcc tggccgggct ggcagcggtg cgccagcctt gcgcgccggg gcgggcgggc
    22921 gcccgggtgc gggcgctggg cctgctgggg ctgggcggga tcgccggttt ctggatcgat
    22981 ggcgccggcg ccatgggggc ggccggcgcg ctgtccttgt ggaaccacca gaacctgcgc
    23041 ctggcgcgct gggcctggcc gggctggagc tttcttgccg gcgcggcggt ggtgctggcc
    23101 cggctgccag agctggcgca gggctgatcg ccggccgcct cagtcttcca gatcgggcgc
    23161 ccaggccacc ccgttgatat gcgcgccgcc atcgacgaac agcgtgttgc ccgtgagata
    23221 gcgcgcgtcc tcgctggcga gaaacaccgc cacgccggca atatcggcgg caggatcgcc
    23281 cagccggccc atcggattgg cggcatggat gcgttcgatc atgcccggct ggccggccat
    23341 caccagcctt gtggaagcgg ccatcgccgc cgggcagatc atgttgcagc agatgcccag
    23401 cggcgcccat tcgcgcgcgg cggtgcgggt gtaggcgcgc atggcttcct tggcggcgtt
    23461 gtagtcggcg ctgcccatat gggcgttcac cccgttcagc gaggccatgt tgatcacccg
    23521 gccccagtgc tgcgccttca tgtggggcag ggcggcttcc attgtccatt tggcggcaaa
    23581 cagggccatg cgcaaagagg cctcgaacac cgcatcgctc ttggcctcga tgcgggccat
    23641 gccctggctg gcccaggcat tgttgacgag gatatccaca gggccggcct cggccatcat
    23701 ggccagaacg gcggcgcgat cggtcacgtc cacgggcagg aagcgggcct ggtgcccggc
    23761 ggcgcggatg tcttgcgcgc aggcgttgcc gcgcgcggca tcatattcgg cgacgatcac
    23821 gctggcgcct tgtgcggcca tgcgctgggc catggcgcgg ccaatgccat cgcccgcccc
    23881 ggtgatcagc gccgtgcggc cggcgagggt gcctgtcatc tcttgctctc cacgataggt
    23941 gttggcttgc gccttcgaga ttgcgcaaat ttatttgccg taaattatga aatgcgtata
    24001 aaaatgcaaa ttataaaacg caaatatcgt atggaggtgg aatgatgcgt ggattgcagg
    24061 gcaaagtggc gatcgtggcc gggggtgggc ggggcattgg cgctgccacc gcccggcgcc
    24121 ttgccgagga aggggccgcg gtggtgatcg gcgatctgct ggtcgatctg gcgggggagg
    24181 tggccagcac gatcgtggcg gcgggcggcc gcgcgctggc cgtgccgctg gatggcaccg
    24241 atgccgcctc gcaaggggcc attgtggcgg cggcctgcag ccatttcggc gggcttgata
    24301 tctatcactc gaatctggcc ggcggcaccg agggcgatat cgatgccctg aactgccccg
    24361 tggaagtgct ggaaaagagc tttgccatca acaccaagag ccatttcctg gcgacccagg
    24421 cggcgctgcc cgcgctgctg gaacgcggcg gcggggcgat gatctacacc tcttcgggcg
    24481 ccgcggccgc cggcgcaccc tggcaggtgg cctatccgat gacgaagaac gccatccacg
    24541 cgctggcccg ccatgtggcg gccaaatggg gcaagcaggg cattcgcgcc aatgtcatct
    24601 gcccggggct ggtgttgacg gaagcggcgc agatgcacct gaccgatgcc tatcgggagc
    24661 aggggctgaa ggccattccg cacaagcggc tgggcaaggc ggaggatatt gccgcggccg
    24721 tcaccttcct tgcctcggaa gatggcgaat gggtcaacgg gcaggtgtgg cacgtcaacg
    24781 gcggcaacat caagcgcgac tgatcgggcg ctgcgccggc ctgcccatgc aaggccggcg
    24841 cagccgcact cagaaggcgg tggcgccgcc atcgaccacc agcgggtggc cggtgacgaa
    24901 gctggcctcg tcggaacaca gccacagcac cgccgcggca atctcgcgcg cttcgcccat
    24961 gcggcccagc ggggtcaggc tgtccatcgt cttgcggatg tcgggattgg cggccagcgg
    25021 cgcggtcatc ggtgtttcga tcacccccgg gcacacggcg ttgacgcgaa tgcccccctt
    25081 ggcccagcgc agcgcgccat ggcgcgtgag gcccaccacg ccatgcttgg tggcggtgta
    25141 ggccggctgc atgccattgc ccaccagccc gttgatcgag gcggtgttga cgatggcccc
    25201 cttgccctgg cgcagcatga tcgccgattg ctcgcgcatg caatacatca cgcccgacag
    25261 gttgacagaa aggctgagcg cccaggcctc atcctgatat tcatcgctgc cgatgcggtt
    25321 gatgccggca ttgttgtggg caaaatccag ccggccatag gcggcttcgg cgcgggccat
    25381 cagcgctttc acttcggcgg catcgcccac gttgcaatgc tggtagaggg ccttgccgcc
    25441 ggcggcggtg atcatctcca ccgtttcggc gccgccggca tcgttgacat ccgacaccac
    25501 caccgccgcg cccgaggcgg caaaggcgat ggccgtggcc cgcccgatgc caccactggc
    25561 accggtgacc accgcgacct tgccgtcaaa atcatatgtc atgcagcctc tccgagtaat
    25621 gcgtagattt atggttcatc attaacgcaa atatcggaag ataaggcaaa tgaaatgcgc
    25681 attcgcgaat ggatgggccc ggcagggcgc ggtctggccc ggcccggccg ttgctgtcag
    25741 gccgcgcggg cgccggggcg gcggtcgatg gtggcgtgaa tgccgttcac ccggtcgcgg
    25801 aattcgccgg cgcgggcgcg cgggtggtag aactggcgat accagatgcg ctccttgtgg
    25861 aaggggccgt catcggggat ctgcaggatg gagagggccg gctccttgtg ctgccacagc
    25921 tcgaaatcct gcgcgaaagc ctccagcgcc acatcctgat agctgcgggc catggcgatg
    25981 ccggcggcat cggccgggcc atcggccacc tttaccatca gcccatacca cacgcgtacc
    26041 atgccatctt ccaccggcgt gtggcagatc atgatgaccg aggggtgttc cccttccatg
    26101 cgcgagagga ggatgccggg gccggtatac caagtgtcgg tggcgagcac ggcggcggtg
    26161 tcggaaacca gcgtgcgatg gcccgagccg aagcgctgca tgatcacgtg atcggcaaat
    26221 tcattctcga aatagaggat gtcgcggctg ccgtgaacgg ggatgaaatg ggcgatgtcg
    26281 gccatgttgt cgaggatttc ctgcgggtgg accggcagcg tgcccagcgc atcgatccgc
    26341 cagcgcaccc agcccgctgt ctcgccatcc cattcggcaa aggcgggcag gtcgaattcg
    26401 ggcgcggcgc cttcctcatc gtgccacatc cacaggcagc cggcccgctc tgccaccggg
    26461 aaggtcttga tgcaggccgc cttgggcacg ccgcgggtgg cataggggat atcgtcgcac
    26521 tggccttcgg gggtgaagcg ccagccatgg ccggggcagc ggatcgcctc gccatcgatc
    26581 tggttgccat cgcgcacgat ataggaagtg gtgttgcggc cgatatgcac gcgcatgtgc
    26641 gggcaatagg catccaccag ataggccttg ccgctttggc cgcgataggc aacgaattca
    26701 cgcccgaaga aacgcagcgg caccggcttg tcggcgatat cgctgctctg ggcgatcatg
    26761 aaccatccgc gcgggaaggt gtgcgggccc agcccatagt cggcggtggt ggccatctgt
    26821 ctctctcctt ggcttgtgcc cgggcgcggc catgcgcggc gggggtgtgc aagggaggtt
    26881 agatgggccg gcgccgcagg ggctttgacg gcgatcaaga tcctcgacga atgcgtctat
    26941 attcttccat ttttctacgc aaacaaatgt ggtttggcgc ttttatgccg cttcctcgtc
    27001 gtcttcctgg atggcgcggg cggtgatccg ggccatgcgg gcgttgtatt tcagcgtctt
    27061 caccgcgatc gaggtttcga tatgctgcac gcccgcaatc gccagtatgc ggtccgaggc
    27121 cagctcgatc agatagtcca gctcgtcgaa caggcacagc gccaggatgt tgaactggcc
    27181 catggtgatc atcaccgcat tgatcagcgg catgtcggcg atctcgcggg cgatctcgcg
    27241 cacgcgctgc acatcggcct gcacgctgat gaaggcaagg cgcgaccggt cgatcgtgtt
    27301 gaagctggtg atggcggtga aggcgatcag cccatcctgc tgcaggcgct tgatgcgccc
    27361 gcgcacggtg ccttcggtca cgcccagctc gctggccacc ttgcggttgg agacgcgcgc
    27421 atcgcgcgag agcagggtga tcagctcgcg gtcgagctcg tcgatctgga caagggccat
    27481 ggcgttatcc cgtgctcagc tcttcgcctc gatgggggcg acatcgaact ggtatttgac
    27541 gatatcgacg accaccgagg gcaccagcgc gcgaatgcca tcgacctgcg agaacttgtc
    27601 gagcagcagg ccgggcaggt cttccagccc gtgcagcgcc accagcatgt cgatatcgta
    27661 gcggccggtg acgagatggg tggcaaacac ctcgggaaat tcggccaggg cggcggccac
    27721 ctgcgaggcc ggccggccat ccacctcgac catcacccgc atcaggcagt tgaagccatg
    27781 ggcggaaaaa tccgacaccg ccaccacgcg cagctggttg gcctcttcca tgcggcggat
    27841 gcgcgaggag acggtagtgg ccgtcagccc cagcagttcg gcgatctgct ggttggaggc
    27901 gcggccatgg gtgcgcaggt gctcgatgat cttgcggtcg atggcgtcaa gcgggatgga
    27961 ttgtgtcatg atagccagcg ggcccccttc atcggcaaga cctgcgcggc cctgcgctcc
    28021 gcgtcaactg taatatgcgt taatttaaag cggatcggct gcgcgaatgc ggtatgatac
    28081 gggggagcac ccagccgcat tcgcgcgaaa gaggaaaaag cgggctcagt cgaaattggc
    28141 ctgcccgcca tcgagcggga tcgtggcgcc ggtgagatag gacagatccg ccccgcacag
    28201 ggccaccacc gcccggccga tatcctcttc cagccggccg atccgcccca ggggaatggt
    28261 gcggaagaag gcttcggctt cctcgggatt gttggcgatc caccatttga ggccggggga
    28321 ttcggcatgg ggggcaatcg ccagcacgcg gatgttctcg cgcccccatt cggcggccgc
    28381 ggcccgggta agcgcgcgga tcgcctgctt caccgcccca taggcgccat agcccgacat
    28441 atcccagcgg atgccggccg aggaggccag gttgatgatc gtgccgccgc cgcgggcggc
    28501 catctgcggg cgggccagct tcatcagccg gaaactggcc agcgggccgg attcaaaacc
    28561 gccgaggaag gcctcgtcgc tcacttcgct cagctggccg ttgggcactt cctgcgcatt
    28621 gttgacgagg atatcgagcc cgccccaggc ctgggcctgg gccatggccg ccgcaatgtc
    28681 ggccgctgcg cgcacgttgc aggcaagggc ccgggcgctg ccgccgcgcg cggtgatgtc
    28741 ggccgcggcc cgttccacct tgtccagcgt gcggccggcc aggatcacgt ctgcgcccgc
    28801 tgccgccagc gccgaggcaa tacccagccc caccccctgc ccggcgccgg tgaccagcgc
    28861 ccgtttgccg cccagatcgg tcatggtctc aatccttcct gcctgtcagc cggcgcgctg
    28921 gcgcagcatg tcgagcgcca catcggtgat catgtcttcc tggccgccca ccatcttgcg
    28981 ccggcccagc tcgacgagga tctcgcgcgt gtcgatgcca tattcggcgg ccgctttctc
    29041 ggcatggcgc aggaagctgg aatagatgcc ggcatagccc agcgtcagcg tctcgcggtc
    29101 cacccgcacg gggcggtcct gcagggggcg cacgaggtct tcggcggcat ccatcagcgc
    29161 catcacgtcg cagccgtggt tccagccctt gcggttggcc gcggcaatga acacctccag
    29221 cggcgcattg ccggcgcccg cgcccatgcc cgccaggctg gcatcgatgc gcatggcgcc
    29281 ctgctgcgcc gccacgatgc tgttggccac gcccagcgag aggttgtgat gggcatggat
    29341 gccgcgctgg gtttcgggct tcagcgcgcg gtcataggcc tccagccggg ccgtcacccc
    29401 atccatgtcg agcgcgccgc cgctgtcggt gacgtagacg caatgcgcgc catagctttc
    29461 catcagcgcg gcctgttcgg cgaggcgttc gggcgcgatc atgtggctca tcatcaggaa
    29521 gccggcaaca tccatgccca gatcgcgggc gatgccgata tgctgacggc tgacatccgc
    29581 ctcggtgcaa tgggtggcca cgcgcaccga gcgcacgccg atcgcatggg cgcgcttcag
    29641 ctcctccacc gtgcccacgc cgggcaggat cagcgtggtc agcaccgcgc gctccagcac
    29701 ttcggccacc gcttccagcc attcccaatc ggtgtgggcg ccaaagccat agttgaagct
    29761 ggcgcccgac agaccatcgc catgggccac ctcgatggca tccacgccgg ccgcatccag
    29821 cgcgcgggcg atcgcgcgca cggcatccag gccatacatg tggcgcacgg catgcatgcc
    29881 atcgcgcagg gtgacatcct ggatatagag ccggtcgccg gcgtctgcgt tgaagcgcgg
    29941 gggggtggcg ctggccatca tgcgttctcc tggcgggcga ggcggcgctg ggcgatgcgc
    30001 tctgcggtgg ccttggcggc ggcggtcatg atgtcgagat tgccggaata ggggggcaga
    30061 taatcgcccg cgccttccac ttccagcagg atcatcgtct tcagccccct gaactggccg
    30121 aggccgggga tggtgagcgg gttgttgtcg ccaaagcgtt cgaactgcac ttcctgcttc
    30181 agccgatagc cgggcacata ggcctgcacc tcggccacca tggcggcaac gctggcgcgg
    30241 atctcttcct cgctggcccc ttccgacagg gtgaagacgg tgtcgcgcat gatcatcggc
    30301 ggctcggccg ggttgaggat gatgatcgcc ttgcccttcg cagcgccgcc cacggcctcg
    30361 atggcgcggg cggtggtgcg ggtgaactcg tcgatattgg cgcgcgtgcc cggccctgcc
    30421 gagcgcgagg agacactggc gacgatctcg gcataatgca ccctggccac gcggctgacc
    30481 gcggccacca tcgggatcgt ggcctgcccg ccgcaggtca ccatgttgac attggtggca
    30541 tcggggttgg catccatgtt caccggcggc acgatgaagg ggccaatggc cgccggcgtc
    30601 agatcgacca catgcttgcc atcggcgcgc agggcctcgt cgtgcagctt gtgggcatag
    30661 gcgctggtgg catcgaagac gatgccgatc tcggcgtagc agggcagggc cttcagcccc
    30721 tccagccctt catgggtggt ggcgatgcca tgttcgcggg ccctggccag cccttcggag
    30781 gcggcatcga tgcccaccac cgcggccagt tccatgttct ggggatattt gatcatcttc
    30841 atcatcagat cggtgccgat gttgcccgat ccgatgatgg cggccttgac ccgttccatc
    30901 agccttccat ccttacacaa agcgggcggc gcagcggccg atggggccct gcctgccgct
    30961 cagaaccatt tcgaagtgat cgccgggcgc ggccggcgcc agcggcacca ggctgcccga
    31021 cagaatgatg tcgccggcat ccagggtgac gccatgggcg cccagcgtgt tggccagcca
    31081 ggccaccgcc gctgccggat cgccctgcac ggcatggccg aagccttcgc tcagcggcgc
    31141 gccattcttg ctgacggcga catgcaggcc gggcagatcg agccctgccg gatccagccg
    31201 ctcctcgccc agcacgaaga ccccgcagga ggcattgtcg gccacggtgt cgacaatgcc
    31261 gatctgccag ttgtcgatcc gcgaatcgac gatctcgaaa cagggggcaa tgctggcggt
    31321 ggccgccagc acatcggccg cggtaatccc cggcccgttg aggctgtgct tcaggatgaa
    31381 ggcgatctcg gcctcggcgc gcggggcgat caggccatgg gcggcgatgt cgatccggcc
    31441 ctgcaccgcc atccggtcgg tcaggaagcc gaaatcgggc tggtgcacgc ccagcatgtc
    31501 ctgcaccgcc ttgctggtca cgccgatctt cttgcccacc accttttcgc cccgcgcgcg
    31561 gcgatggcca aggaaggcca ggctgatggc ataggcatcc tcgatggtaa gcgagggatc
    31621 ggccgcgctg atcggcgcga tggtgcggcg cgcttccatc gccgcgaaca gggcaaggcc
    31681 cagttcggcc gtgcgcgcgg tgctggcagc ggcgtggctc atgcccgcgc cttcaggaaa
    31741 tcgagcgtat aggcgttgaa ttcggcggcg cgttccacct gcacccaatg gcccgtgcgg
    31801 gcaaaggtga tggcgcgcac atcggcgcag gcatccagca gcagggcggc atggctggcc
    31861 gggcagaagg catcctcgcg cccccacagc acgaagaccg gcgccgtgat ctcgcccagc
    31921 cgcggcgaca gatccggcgt gcgcatgcgc accagcacct ccttgggctg gctgctggcc
    31981 acggcaaagc gttcggccac cagcgcgtcg gtgatcagcc cgtgggcatc ggggtgcagc
    32041 aggttggcga tcagccgctg ctgttcggcc aggttgaaat ccggcccgcc gaaggaggag
    32101 accatgccgg caatccccgg catggcaaaa taggcctcgc gcggggccag gcagcccggt
    32161 gccatcagca ccagcgcgcg ggccaggccg ggccgatcca gcgcggcctg gatggcaatg
    32221 ccgccgccca gcgaattgcc gaccaggatc gcctctgcaa tcccgtgcag gtccagcgct
    32281 tccagcagcg tgtcggtgaa cagctgcagc gtgtagtcga tcccgtccgg cttcgaggac
    32341 gcgccatagc cgatgagatc gggcaggatc acgcggtagc cggccgccac gaaggcctcg
    32401 atgttctggc ggaaattgga agcgcccgaa gcgcccggcc cgctgccgtg gatgaacacc
    32461 accgccggcc ccgcgcccgc ttcctgcagc acgatgctgt gcccgccaga caaccgatgc
    32521 gttgttttgg tcaatgtcgt catgtgttgt tgtctcacag gaagttgaag cttggttggt
    32581 ggccgagagc ggtgttgacg aggtcgctgg cgcggttgtt ggggtcgttg gcgacgtgtg
    32641 cgcggccggc gttgaggtcg agccagggct ggatgatggg gcttg
//
LOCUS       DASRYE010000343        15255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_176328,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000343 DASRYE010000000
VERSION     DASRYE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176328"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFF98_17935"
     CDS             <1..351
                     /locus_tag="VFF98_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65574.1"
                     /translation="PGSRPAPPPRFAPAKPLGKGLGRGHAMPHVLSLPGLEGVIGADA
                     RGLVAQFGPARLDVHEGEAQKLQFAGEACVLDVYLYPGEGGGPARATYVDARRASDGR
                     DVDRAGCVAALKRR"
     gene            451..822
                     /locus_tag="VFF98_17940"
     CDS             451..822
                     /locus_tag="VFF98_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65575.1"
                     /translation="MPLFSPRAAPPGADAPLHPRLDADTVDALLAIDADLAAPGAEWA
                     EFVGEAVSHWLVEQRAPAGDLDVAKTRWLLRRIDRGGQVQSRAALLLLQRCVVRARRV
                     SGELMAYLRRQEEDLRLPLAA"
     gene            complement(855..1229)
                     /locus_tag="VFF98_17945"
     CDS             complement(855..1229)
                     /locus_tag="VFF98_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZU65576.1"
                     /translation="MSDAAVPTQTPRRPRPAAVALTANAEARIGRLMAEAPEGTIGVK
                     LSTPRRGCSGLAYSVDYVSAASPLDERIETAGGLFFIDSASVLYLVGSTMDWVEDDFS
                     AGFVFANPNAKGSCGCGESFTV"
     gene            complement(1229..1720)
                     /locus_tag="VFF98_17950"
     CDS             complement(1229..1720)
                     /locus_tag="VFF98_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HZU65577.1"
                     /translation="MSESEKPRFTVEEVNAAPTPPRARVDQADAPAENPADTLARKKD
                     YLEGFIAQQPTGLAAHEPGGALYEGVVGALKEIYDPEIPVNIYELGLIYGVEITAEGA
                     ASITMTLTTPHCPVAESMPGEVEMRVAAVPGVRDCEVSLVWDPPWDPAKMSDEARLEL
                     GML"
     gene            complement(1717..2934)
                     /locus_tag="VFF98_17955"
     CDS             complement(1717..2934)
                     /locus_tag="VFF98_17955"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZU65578.1"
                     /translation="MAEPLDLAAIRADFPGLVTADGRPWHYLDSGATAQKPRAVIEAC
                     ARAMGADYATVHRGVYARSAEMTLAYEAARRRVASFIGGAEEEIVFTRGATEAINLVA
                     YSWGMANLRAGDRILLSTLEHHSNIVPWQLIQEKIGFAIDVCPLTADGRIDLDAAENL
                     LTPAHKLVAFAHVSNVLGSVLDVQRAVALGHAVGAKVLIDGCQAVPRLKVDVAALGCD
                     FYAFSAHKLYGPTGIGALWARADILAGMPAWQGGGSMIDRVSFEKTTYAPAPTRFEAG
                     TPAIVEALGLAAAIDYVETIGLGAIHAHEARLVRRLRGELAAMNSVRLFGPDDSAGIV
                     SFELEGVHPHDLGTILDEAGVAIRAGHHCAQPLMAHLGVPATARASFGLYSDDSDIAA
                     LLAGIERTKRIFG"
     gene            complement(2937..3671)
                     /locus_tag="VFF98_17960"
     CDS             complement(2937..3671)
                     /locus_tag="VFF98_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HZU65579.1"
                     /translation="MSALAPLPTRKAEDWRYADLAALEKLWPLAEAEAVVVGAGGHHA
                     RAIVQTGGGITRLALTLEAGASAVLHVLNAGGAYGRVELDVTLHEGADFTLGAVQIAG
                     SGETAEIIATVRHMEGKATSRQVVRQAAGGTGTVTCLGKVYVAKGADGTDGEQSVRAM
                     LLDRTATANARPELEIHADDVKCAHGCAIGELDANGLFYMAARGIAPETAKALMLQGF
                     IAEALEGAEEEDHLRALAQQALEAVL"
     gene            complement(3668..4411)
                     /gene="sufC"
                     /locus_tag="VFF98_17965"
     CDS             complement(3668..4411)
                     /gene="sufC"
                     /locus_tag="VFF98_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZU65580.1"
                     /translation="MLKIENLHAAIADKPILKGLSLSINAGEVHAIMGPNGAGKSTTG
                     YVLGGRPGYEVTEGSATFNGADLFALEPHERAAAGLFLGFQYPVEIPGVSNLQFLREA
                     LNSQRKLRGEEPLSGGAFLKIAREKAALLKMDMEMLKRPVNVGFSGGEKKRAEMVQMG
                     ILDPKLAILDETDSGLDIDALRICGEGINAIMRKPDKAVLLITHYQRLLDYVKPDFVH
                     VLAGGRIVKSGGPELALELEEHGYEDVAA"
     gene            complement(4411..5916)
                     /gene="sufB"
                     /locus_tag="VFF98_17970"
     CDS             complement(4411..5916)
                     /gene="sufB"
                     /locus_tag="VFF98_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZU65581.1"
                     /translation="MTQPIDTETPADGALKDQAAREAAQKVAEYEHGWVADIEQEYGP
                     KGLSEDTVRFISAKKDEPEWMLEWRLKAYRHWLTMPMPDWAKLNVPPIDYQNAYYWAA
                     PKQKDGPKSLDEVDPEILRVYEKLGIPLEEQKVLAGVEGARKVAVDAVFDSVSVATTF
                     RKELEAAGVIFRSISEAIREYPELVKKWLGRVVPMADNFFAALNCAVFSDGTFVYIPE
                     GVRCPMELSTYFRINAENTGQFERTLIVADKGAYVSYLEGCTAPQRDENQLHAAVVEL
                     VALDDAEIKYSTVQNWYPGDAQGKGGIYNFVTKRALCQGARSKVSWTQVETGSAITWK
                     YPSCVLNGEDSVGEFYSVAVTNNYQQADTGTKMIHNGRGSRSTIISKGISAGKSQNTY
                     RGLVRVGAAAEGVRNFTQCDSLLLGKECGAHTVPYIEVRNPTATIEHEATTSKISDDQ
                     LFYAQQRGLDTEAAVALIVNGFAKEVLQQLPMEFAVEAQKLLGISLEGSVG"
     gene            complement(5913..6395)
                     /locus_tag="VFF98_17975"
     CDS             complement(5913..6395)
                     /locus_tag="VFF98_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZU65582.1"
                     /translation="MRLSSMADYAVVTMVAAARHCGSAARLAAESAGQRRPHLNAGLL
                     AEETGLPAPTVQKLVSRLVVAGLLKSTRGVGGGLRLARPAAAISLAEIVEAVEGPIAL
                     ATCVDTAKQECALEQGCSVRPHWPVVNEALRGALAGVPLSRLACLPVPAPDKMMEAMQ
                     "
     gene            6883..7923
                     /locus_tag="VFF98_17980"
     CDS             6883..7923
                     /locus_tag="VFF98_17980"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZU65583.1"
                     /translation="MYYALLRPLLFRLDAERAHGLSIAALKCLPLGRACRGAQSPLAI
                     SVAGITFPNPLGMAAGFDKDAEVPDQLLGLGFGFAEAGSITPLPQEGNARPRLFRLAE
                     DRAVINRMGFNNHGAEAALARLEMRKGLGGVVGINIGANKDSADRIADYATMARLMAP
                     HASYLAVNVSSPNTPGLRALQDEKALADLLDGVIAARDETGCAPPVFLKVAPDLLPAD
                     IDAIARLAIEKRLGALIVANTTISRPPLRSRHAGEGGGLSGAPLKELALERLRDFRKA
                     TGGAVPLVGVGGIATAEDAWQRIRAGASLVQLYSAMVYAGPGLARQITRGLEKRMLAE
                     GFACLADAVGTA"
     gene            8089..9891
                     /locus_tag="VFF98_17985"
     CDS             8089..9891
                     /locus_tag="VFF98_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HZU65584.1"
                     /translation="MQLSDFDSSANIVALFLARAAALGDRPFLWAKQDGRWQSVSWAE
                     AARQMCLLAEALAGLGLKPGERVMLVSENRPEWCISDLAIMAAGCISVPTYTTNTERD
                     HLHILENSGARAVIVSSARLARPLLPAVLRAGKADHVISFEPLRPAQASTVEYHDWAA
                     LVAGDAAAAMAAVEARMARITRQDIACIIYTSGTGGAPRGVPQHHGALLHNVDGTARI
                     IAEDFGWEEEVFLSFLPLSHSYEHTGGQFLPIGLGGQIWYAEGLEKLASNIEEARPTI
                     MIVVPRLFEVLRTRIIKQIEKQGALARLLMEKAVQMSLRHQQGRRHLFDGALNMVLER
                     TLRPKIRARFGGRMKALVSGGAPLNPEVGTFFEAMGMTLLQGYGQTESGPVISVGRPG
                     AGLKMDTVGPPLRNTQVKIAGDGEILVAGELVMQGYWRNEAETAKTIQDGWLHTGDIG
                     EIDAVGRIRITDRKKDMIVNDKGDNISPQKLEGMLTLQPEIAQAMVIGDRRPYVVGLI
                     VPDAEWALDWARSQDVRFDMRSLQDLPAFRSAMRAAIDRVNADLSVIEKLRQFTFAEE
                     PFSIDNGEMTPSLKIRRHKLKERYGERLEGLYRS"
     gene            complement(9936..10478)
                     /locus_tag="VFF98_17990"
     CDS             complement(9936..10478)
                     /locus_tag="VFF98_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65585.1"
                     /translation="MAHTGMASLAIAELREFAGFTPCEQRYIKRSLDVGLGRQDAFKL
                     WARDALEAASIRSQYVLYQELKGLRGAIPAHGGPAVPGMESLESFMGKLIRLTAFDLA
                     QDKLSGFAPFRFLYERLLGADVRPWLPSAFCAAAALPQIRPERRKRLLQSISEAAATA
                     PGWSTREPWFYPEWVEKEAA"
     gene            complement(10640..11629)
                     /gene="thiS"
                     /locus_tag="VFF98_17995"
     CDS             complement(10640..11629)
                     /gene="thiS"
                     /locus_tag="VFF98_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZU65586.1"
                     /translation="MTDAISLTVNGEPRRIRAGASVAELVADIGLNPAKVAVERNGAI
                     VPRSTLADVALNAGDILEIVHFVGGGEVSADDSWSVAGRTFRSRLIVGTGKYRDFAQN
                     AAALEASGAEIVTVAVRRVNIADPAAPMLTDFIDPKKITYLPNTAGCFTADDAIRTLR
                     LAREAGGWDLVKLEVLGEARTLYPDMRETLKATEVLAREGFLPMVYCVDDPIAAKQLE
                     EAGAVAIMPLGAPIGSGLGIQNRVTIRLIVEGAKVPVLVDAGVGTASDAAIAMELGVD
                     GVLMNTAIAEAKDPIRMARAMKLAVEAGREAYLSGRMATRKYADPSSPLAGLI"
     gene            complement(11687..12100)
                     /locus_tag="VFF98_18000"
     CDS             complement(11687..12100)
                     /locus_tag="VFF98_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HZU65587.1"
                     /translation="MAAHPSLIRRGVDRLGIALSAACAVHCVAGLALAAVLGLGATAV
                     GTGALATSTLGTALLDPRIHEYGLMLAVAVGALGLGVGAARHRQPGLLLLGLAGLALM
                     SAGVLAPHGPLEAGFTIPGVLLLAMAHLRNLRHAH"
     gene            12199..13266
                     /locus_tag="VFF98_18005"
     CDS             12199..13266
                     /locus_tag="VFF98_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZU65588.1"
                     /translation="MSSKRSGTVLSFPHGAARRRGLPALLALWLAVVAALVVAIVVVG
                     GITRLTESGLSITEWKPLSGIVPPLNEAAWAAEFAHYRQIDQYGAVHAGMTLAQFKGI
                     FFWEYIHRVLGRVIGMAVLVPLVWFAWRRHIPAGYGWRLLALLALIGLQGTLGWLMVA
                     SGLKPGMTEVAPLWLMAHLLTALFTLAGLVWTALDLVALAGHARARPARLTGLGLLAL
                     LGLWVQLGFGALMAGLRAGRVTDQWPLMNGSFWPGATQPRASLLAYLTADPQIVHFIH
                     RWWAWGVVALLVVLARRVKAAGARGAAFAIHCAFGTQVLLGIATVMAGVPVWLGVLHQ
                     LVGALLVVSVVWGVHALGRRG"
     gene            13263..13589
                     /gene="cutA"
                     /locus_tag="VFF98_18010"
     CDS             13263..13589
                     /gene="cutA"
                     /locus_tag="VFF98_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZU65589.1"
                     /translation="MNGQGAALIWCPFADEASAAAVAGALLDEGLVACVNILPAVRSL
                     YIWQGARGEGAEVGALFKTRADLLEAAIARIAALHPYEAPAVVGWHCDGAAAPTLAWL
                     GALTGG"
     gene            13723..14202
                     /gene="rplM"
                     /locus_tag="VFF98_18015"
     CDS             13723..14202
                     /gene="rplM"
                     /locus_tag="VFF98_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZU65590.1"
                     /translation="MKALTKVTQSAKPAEVEKKWHLIDADGLVVGRVAVIIANILRGK
                     HKPSFTPHVDCGDHVVVINADKVKFTGNKLKQQTYYKHTGYAGGIKEVTADKVLAGRF
                     PERVLEKAVERMIPRGPLGRAQMRALHLYAGAEHPHAGNQPEVLDVAARNRKNKVGA"
     gene            14202..14693
                     /gene="rpsI"
                     /locus_tag="VFF98_18020"
     CDS             14202..14693
                     /gene="rpsI"
                     /locus_tag="VFF98_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZU65591.1"
                     /translation="MSEEFSALADLGTDLGAPAAPSAPAAPLREREVDAQGRSYATGR
                     RKDAVARVWIKPGTGKITVNGRDQEVYFARPTLRLVINQPFGIADRSGQYDVVCTVKG
                     GGLSGQAGAVKHGIAQALAKFEPALRSTVKAAGFLTRDPRVVERKKYGRAKARRSFQF
                     SKR"
BASE COUNT         2375 a         4928 c         5315 g         2637 t
ORIGIN      
        1 ccggggtcgc gccccgcgcc gccgccgcgc tttgccccgg ccaagccgct gggcaaggga
       61 ctgggcaggg ggcatgcgat gccgcatgtg ctgtccctgc cggggctcga aggggtgatt
      121 ggcgccgatg cccgcggcct ggtggcgcaa ttcggcccgg cgcggctgga tgtgcatgaa
      181 ggcgaggcgc aaaagctgca atttgccggc gaggcctgcg tgctcgatgt ctatctctat
      241 ccgggcgaag ggggcgggcc ggcgcgcgcc acctatgtcg atgcccgccg cgccagcgat
      301 ggccgcgatg tggacagggc cggctgcgtg gccgcgctga agcggcgctg agctttccta
      361 acgctggctg aaaatccggt tcatcgcgcg ctcaggcggg gctgcgcatg gtggcggcac
      421 cgggggaaat gccagacgta aggatcgatg atgccactct tttcccctcg cgccgcgccg
      481 cccggcgccg atgccccgct gcacccgcgc cttgatgccg atacggtgga tgcgctgctg
      541 gcgatcgatg ccgatctggc cgcgcccggc gccgaatggg ccgaatttgt tggcgaggcg
      601 gtgtcgcact ggctggtgga gcagcgcgcg ccggcaggtg atctcgatgt tgccaaaacc
      661 cgctggctgc tgcgccggat cgatcgcggc gggcaggtgc aatcgcgcgc ggccttgctg
      721 ttgctgcagc gctgcgtggt gcgggcgcgc cgcgtttcgg gtgagctgat ggcctatctg
      781 cgccggcagg aagaggatct gcgcttgccg ctggccgcct gaaaggggcg gccagcggtg
      841 gctgcaggcg gtgcttacac cgtgaagctt tcgccgcagc cgcagctgcc cttggcgttg
      901 gggttggcaa agacgaagcc ggcggaaaaa tcatcttcca cccaatccat cgtgctgccc
      961 accagataga gcacgctggc gctgtcgatg aagaacaggc cgccggccgt ctcgatccgc
     1021 tcgtcaagcg ggctggccgc gctgacgtaa tcgaccgaat aggcaaggcc cgagcagccc
     1081 cggcgcggag tggacagctt gacgccgatc gtgccttcgg gcgcctcggc catcagccgg
     1141 ccgatgcgcg cctcggcatt ggcggtgagg gcaacggcgg ccgggcgggg ccggcgcggc
     1201 gtttgggtgg gcacggcggc atcgctcatc acagcatccc cagttcgagg cgggcctcat
     1261 cgctcatctt ggccggatcc catggcggat cccacaccag gctcacttcg caatcgcgca
     1321 cgccgggcac ggcggccacg cgcatctcca cttcgccggg catcgattcg gccaccgggc
     1381 agtggggggt ggtcagcgtc atggtgatgc tggccgcgcc ctccgcagtg atctccacgc
     1441 catagatcag gcccagctcg taaatgttca ccgggatctc ggggtcgtag atctccttca
     1501 gcgcgcccac cacgccttca tagagcgcgc cgcccggctc atgcgcggca aggccagtgg
     1561 gctgctgggc gatgaagcct tccagataat ccttcttgcg ggccagcgtg tcggccgggt
     1621 tttccgccgg cgcatcggcc tgatccaccc gcgcgcgggg cggggtgggg gcggcgttca
     1681 cttcctccac cgtgaagcgg ggcttttcgc tctcgctcat ccgaagatcc ttttcgtccg
     1741 ttcgatgccg gccagaagcg cggcgatatc gctgtcgtca ctatagaggc cgaagctggc
     1801 ccgggcggtg gcgggcacgc ccagatgggc catcagcggc tgggcgcaat ggtggccggc
     1861 gcggatggcc acgcctgcct cgtcgaggat ggtgcccaga tcatgcgggt gcacgccttc
     1921 cagctcgaag gagacgatgc ccgcgctgtc gtccggcccg aacagacgaa cgctgttcat
     1981 cgccgccagt tccccgcgca ggcgccgcac cagcctggcc tcatgggcgt ggatggcgcc
     2041 aaggccgatg gtttccacat aatcgatcgc cgcggccagc cccagcgctt caacgatcgc
     2101 cggcgtgccg gcctcgaagc gggtgggggc gggggcatag gtggtcttct cgaagctcac
     2161 ccggtcgatc atcgagccgc cgccctgcca ggcgggcatc ccggcgagaa tgtcggcccg
     2221 ggcccacaag gcgccaatgc cggtggggcc atacagcttg tgggcggaaa aggcatagaa
     2281 atcgcagccc agcgctgcca catccacctt cagccgcggc accgcctggc agccatcgat
     2341 cagcactttg gcgcccaccg catggcccag cgccaccgcc cgctgcacat ccagcacgct
     2401 gcccagcacg ttcgacacat gggcaaaggc caccagcttg tgcgccggcg tcagcaggtt
     2461 ttcggcggca tcaaggtcga tccggccatc tgccgtcagc gggcagacat cgatggcaaa
     2521 gccgatcttc tcctggatca gctgccaggg cacgatgttc gaatgatgct ccagcgtgga
     2581 aagcaggatg cgatcgcccg cccgcaggtt ggccatgccc cagctatagg ccaccagatt
     2641 gatcgcctcg gtggcgccgc gggtgaagac gatctcctcc tccgccccgc cgatgaagct
     2701 ggccacccgg cggcgcgcgg cctcataggc cagcgtcatc tcggccgagc gggcatagac
     2761 gccgcggtgc acggtggcat aatcggcccc catggcgcgg gcgcaggcct cgatcacggc
     2821 gcgcggcttt tgcgcggtgg cgccggaatc aagatagtgc cacggccgcc catcggcggt
     2881 gacaaggccg gggaaatcgg cgcggatggc ggccaggtcc agcggttcgg ccagggtcac
     2941 agcaccgcct ccagcgcctg ttgcgccagc gcgcgcagat ggtcctcttc ctcggcgcct
     3001 tccagcgctt cggcgatgaa gccctgcagc atcagcgcct tggcggtttc cggcgcaatg
     3061 ccgcgggcgg ccatgtagaa caggccgttg gcatccagct cgccgatcgc gcagccatgg
     3121 gcgcatttca cgtcgtcggc gtggatttcc agctcgggcc gggcattggc ggtggcggtg
     3181 cgatccagca gcatggcgcg cacgctctgc tcgccatcgg tgccatcggc gcccttggcg
     3241 acatagacct tgccaaggca agtgacggtg ccggtgccgc cagcggcctg gcgcacgacc
     3301 tgccggctgg tggccttgcc ctccatgtgg cgcacggtgg cgatgatctc ggcggtctcg
     3361 ccgctgccgg cgatctgcac cgcgcccagc gtgaaatcgg cgccttcatg cagtgtcacg
     3421 tcgagctcca cccggccata ggcgccgccc gcgttcagca catggagcac cgcgctggcg
     3481 cccgcttcca gcgtgagggc gaggcgcgtg atgccgccgc ctgtctgcac gatggcgcgc
     3541 gcatggtggc cgcctgcgcc caccaccacc gcctcggcct cggccagcgg ccacagcttt
     3601 tccagcgcgg ccagatcggc atagcgccaa tcctccgcct tgcgggtggg caggggcgcc
     3661 agcgcgctca tgccgccaca tcctcatagc cgtgctcttc cagctccagc gccagttcgg
     3721 gcccgccgct cttcacgatg cgcccgcccg ccagcacatg gacgaaatcg ggcttcacgt
     3781 aatcgagcag gcgctgataa tgggtgatca gcagcaccgc cttgtcgggc ttcctcatga
     3841 tggcattgat gccttcgccg cagatgcgca gcgcatcaat gtcgaggccg ctgtcggtct
     3901 cgtccagaat cgccagtttg gggtcgagga tgcccatctg caccatctcg gcgcgcttct
     3961 tctcgccgcc ggaaaagccc acgttcaccg ggcgcttcag catctccatg tccatcttga
     4021 gaagggctgc cttctcgcgc gcgatcttca ggaaggcgcc gcccgagagc ggctcttccc
     4081 cgcgcagctt gcgctggctg ttcagcgcct cgcgcaggaa ctgcaggttc gagacgccgg
     4141 ggatctccac cggatactga aagcccagga acaggccggc cgcagcccgc tcgtgcggct
     4201 ccagcgcgaa gagatcggcg ccgttgaacg tggccgaacc ttccgtcacc tcatagcccg
     4261 ggcggccgcc cagcacatag ccggtggtgg atttgcccgc gccattgggc cccatgatgg
     4321 catgcacctc gcccgcgttg atggagagcg aaaggccctt gaggatcggc ttgtccgcga
     4381 tggcggcgtg gaggttttca attttcagca tcagccgaca cttccttcaa gactaattcc
     4441 cagcagcttc tgcgcttcca cggcaaattc catgggcagc tgctgcaaca cttccttggc
     4501 aaagccgttg acgatgagcg ccaccgccgc ctcggtatcc agcccgcgct gctgcgcgta
     4561 gaacagctgg tcgtcggaga tcttgctggt ggtggcttca tgctcgatgg tggcggtggg
     4621 gttcctcacc tcgatatagg gcacggtgtg ggcgccgcat tccttgccca gcagcagcga
     4681 atcgcactgg gtgaagttgc gcacgccctc ggcggccgcg cccacgcgca ccaggccgcg
     4741 ataggtgttc tgcgacttgc cggcgctgat ccccttggag atgatggtgg agcggctgcc
     4801 gcgcccgttg tggatcatct tggtgccggt gtcggcctgc tggtaattgt tggtgacggc
     4861 gacggaatag aactcgccca ccgaatcctc gccattgagc acgcaggagg ggtatttcca
     4921 ggtgatggcg ctgccggttt ccacctgcgt ccagctcacc ttcgatcgcg cgccctggca
     4981 cagcgcccgc ttcgtcacga aattgtagat gccgcccttg ccctgcgcat cgcccggata
     5041 ccagttctgc acggtggaat atttgatctc ggcatcatcc agcgccacca gctccaccac
     5101 ggcggcatgc aactggttct cgtcgcgctg gggggcggtg cagccttcga gataggacac
     5161 ataggcgccc ttgtcggcca cgatcagcgt gcgttcgaac tggccggtgt tctccgcatt
     5221 gatgcggaaa taggtggaca gctccatcgg gcagcgcacg ccctcgggga tgtagacgaa
     5281 ggtgccatcg gagaagaccg cgcaattgag agcggcgaag aaattgtcgg ccatgggcac
     5341 cacgcggccc agccacttct tcaccagctc gggatattcg cggatcgcct cggagatcga
     5401 gcggaagatg acgccggccg cttccagttc cttgcggaag gtggtggcca ccgacacgct
     5461 gtcgaacacc gcatccaccg ccaccttgcg ggcgccttcc acgcctgcca gcaccttctg
     5521 ctcttccagc ggaatgccca gcttctcgta gacgcgcagg atttccgggt ccacctcgtc
     5581 caggctcttg gggccgtcct tctgcttggg cgcggcccag taataggcgt tctggtaatc
     5641 gatcggcggc acgttcagct tggcccagtc gggcatcggc atggtcagcc agtggcgata
     5701 ggccttcagg cgccattcca gcatccattc cggctcgtcc ttcttggccg aaatgaagcg
     5761 gacggtgtct tccgacaggc ccttggggcc atattcctgc tcgatatcgg ccacccagcc
     5821 gtgttcgtat tccgccactt tctgggcggc ctcgcgcgcg gcctggtctt tcagggcgcc
     5881 atcggcgggg gtttcggtgt cgatcggctg ggtcattgca ttgcttccat catcttgtcg
     5941 ggcgcgggca ccggcaggca ggccagccgg ctgagcggca cccctgccag cgcgccgcgc
     6001 agcgcctcgt tcaccaccgg ccagtgcggg cgcaccgagc agccctgttc cagcgcgcat
     6061 tcctgcttgg ccgtgtcgac acaggtcgcc agcgcgatcg gcccttccac cgcctcgacg
     6121 atctcggcca ggctgatggc cgctgccggc cgcgccagcc tgaggccgcc gcccacgccc
     6181 cgcgtggatt tgagcagccc tgccaccacc agccggctca ccagcttttg caccgtgggc
     6241 gcgggcaggc cggtttcctc cgccagcagg ccggcattga ggtgcgggcg gcgctggccg
     6301 gcgctttcgg cggcgaggcg cgcggcgctg ccgcaatggc gggcggcggc caccatcgtc
     6361 accacggcat aatcggccat actcgacaag cgcatcgcag gcggctttcg cgggcggtgg
     6421 aacacgggcg cgcttggcgc gcgccgacgg ctaaactgga tcggatcggt ccacattcta
     6481 tctaggcgcg ttgggggcag atggcaaccg tctgcatggc aggggttggc gcgaaggggg
     6541 gcctgccggc aagcgggcat aaaaaagcgg cctttccaaa aggaaaggcc gccagagttt
     6601 cgagaactcg ggccaggcgg cacgagccct tcgggtcgca atgggggtgt agggcaggag
     6661 gaagcggcaa aacttgtaga tactcgtcac cctccgaaac ggagccgaca aagcagacga
     6721 ttgcaccatt ttttgttcct ccctgttgca gcgcagcgac ggtgcccatg ggcctgtggc
     6781 gggctgccct atcgggcaat ccgccgcggg cgggttttgg ccactttctc cggctatgcc
     6841 cgccgttgcc ttgcgggccc ggcagggcca taggcgaatc gcatgtacta tgcccttttg
     6901 cgcccgttgc tgttccgcct ggatgcggaa cgtgcccatg gcctgtcgat cgccgcgctg
     6961 aaatgcctgc cgctgggcag ggcctgccgg ggcgcgcaaa gcccgctggc gatcagcgtg
     7021 gcgggcatca cctttcccaa tccgctgggc atggcggccg gcttcgacaa ggatgccgaa
     7081 gtgcccgacc agctgctggg gctgggcttc ggctttgccg aggcgggctc gatcacgccg
     7141 ctgccgcagg agggcaatgc gcgcccgcgc ctgttccgcc tggccgagga ccgggcggtg
     7201 atcaaccgca tgggcttcaa caaccacggc gccgaggcgg cgctggcgcg gctggagatg
     7261 cgcaaggggc ttggcggggt ggtgggcatc aacattggtg ccaacaagga ttcggccgat
     7321 cgcatcgccg attatgccac catggcccgg ctgatggcgc cccatgccag ctatctggcg
     7381 gtgaatgtgt ccagccccaa cacgccgggc ctgcgcgcgc tgcaggatga aaaggcgctg
     7441 gccgacctgt tggacggggt gatcgccgcg cgcgatgaaa cgggctgcgc gccgccggtg
     7501 ttcctgaaag tggcgcccga cctgctgccg gccgatatcg acgcgattgc ccgcctcgcc
     7561 atcgagaagc ggctgggcgc gttgatcgtg gccaacacca ccatctcgcg cccgccgctg
     7621 cgcagccgcc atgccggcga gggcggcggc ctttccggcg cgccgctgaa ggaactggcg
     7681 ctggaacgcc tgcgcgattt ccgcaaggcg acgggcggcg cggtgccgct ggtgggcgtg
     7741 ggcggcattg ccactgccga ggatgcctgg cagcgcatcc gtgcaggcgc cagcctggtg
     7801 cagctgtaca gcgcgatggt ctatgccggg cccggccttg cccgccagat cacccgcggg
     7861 ctggaaaagc gcatgcttgc cgagggcttt gcctgcctgg ccgatgcagt gggcacagcc
     7921 tgagggcgta tgcccggtag cggcgggcgc tgcgtaaaca atatgctaaa tccttggcgc
     7981 taggctctta accatgcttg ttctgccggc tgtgcgggcc tagacaggta tatatcacga
     8041 gcagtcgggc ggggggcgtt cgaactgtca gggggaacgg gcgccagcgt gcaactttcc
     8101 gatttcgatt cgtctgccaa tatcgtcgcc ctgtttctcg cgcgggccgc ggcgctgggt
     8161 gacaggcctt ttctctgggc gaagcaggat gggcgctggc aatctgtcag ctgggccgag
     8221 gcggcgcgcc agatgtgcct gctggccgaa gcgctggccg ggctggggct gaagccgggt
     8281 gagcgggtga tgctggtttc ggaaaaccgg ccggaatggt gcatcagcga tctggcgatc
     8341 atggcggccg gctgcatcag cgtgcctacc tacaccacca ataccgagcg cgatcatctc
     8401 cacatcctgg aaaattccgg cgcgcgggcg gtgatcgtct cctcggcgcg gctggcgcgg
     8461 ccactgttgc cggcggtgct gcgggcgggc aaggccgatc atgtgatcag cttcgagccg
     8521 ctgcgcccgg cccaggccag cacggtggaa tatcacgact gggccgcgct ggtggccggc
     8581 gatgcggcag cggcgatggc ggcggtggaa gcgcgcatgg cccggatcac ccgccaggat
     8641 atcgcctgca tcatctacac cagcggcacc ggcggtgccc cgcgcggcgt gccgcagcac
     8701 catggcgcgc tgctgcacaa tgtggatggc accgcgcgca tcatcgccga ggattttggc
     8761 tgggaagagg aagtcttcct ctccttcctg ccgctttccc attcctatga gcataccggc
     8821 gggcagttcc tgccgatcgg ccttggcggg cagatctggt atgccgaagg gctggagaag
     8881 ctggccagca acattgagga agcgcggccg accatcatga tcgtcgtgcc gcgcctgttc
     8941 gaggtgctgc gcacgcggat catcaagcag atcgagaagc agggcgcgct ggctcgcctg
     9001 ctgatggaaa aggcggtgca gatgtcgctg cgccaccagc agggccggcg gcacctgttc
     9061 gatggcgcgc tgaacatggt gctggaacgc acgctgcgcc ccaagatccg cgcgcgcttt
     9121 ggcgggcgga tgaaggcgct ggtgtcgggc ggggcgccgc tcaacccgga ggtgggcact
     9181 ttcttcgagg cgatgggcat gaccttgctg caaggctatg gccagaccga aagcggccct
     9241 gtgatcagcg tgggccggcc gggcgccggg ctgaagatgg acacggtggg cccgcccttg
     9301 cgcaacacgc aggtgaagat cgccggcgat ggcgagatcc tggtggcggg cgagctggtg
     9361 atgcagggct attggcgcaa cgaggcggaa accgcgaaaa ccatccagga tggctggctg
     9421 cacaccggcg acatcggcga gatcgacgcg gttggccgca tccgcatcac cgatcgcaag
     9481 aaggacatga tcgtcaacga caagggcgac aatatctcgc cccagaagct ggagggcatg
     9541 ctgaccctgc agcccgagat cgcccaggcg atggtgatcg gcgaccggcg gccctatgtg
     9601 gtggggctga tcgtgcccga tgcggaatgg gcgctggatt gggcgcgcag ccaggatgtg
     9661 cgcttcgaca tgcgcagcct gcaggatctg ccggccttcc gcagcgccat gcgcgccgcg
     9721 atcgaccggg tgaacgcgga tctgtcggtg atcgagaagc tgcgccagtt cacctttgcc
     9781 gaggagccct tctcgatcga caatggcgag atgacgcctt cgctgaagat ccgccgccac
     9841 aagctgaagg agcgttatgg cgagcggctg gaagggctgt atcgcagcta ggcgaaggct
     9901 gggcgagggg gccgggggcc cctcgcgccg gaagttcagg cggcttcctt ctccacccat
     9961 tcgggataga accagggctc gcgggtggac cagccggggg cagtggcagc cgcctcgctg
    10021 atcgattgca gcaggcgctt gcggcgttcg gggcggatct gcggcagggc cgccgccgcg
    10081 cagaaggccg agggcagcca ggggcgcaca tcggcgccca gcagccgttc gtaaagaaag
    10141 cggaacgggg caaagcccga gagcttgtcc tgcgccagat cgaaagcggt gaggcggatg
    10201 agcttgccca tgaagctttc caggctttcc atgccgggca cggccggccc gccatgcgcc
    10261 gggatcgccc cgcgcaggcc cttcagttcc tgatagagca catactggct gcggatcgag
    10321 gcggcctcga gcgcatcgcg cgcccacagc ttgaaggcat cctgccggcc cagccccaca
    10381 tcaaggctgc gcttgatgta gcgctgttcg caaggggtga agccggcaaa ttcccgcagc
    10441 tccgcgatcg ccagcgatgc cattccagtg tgggccatgt tccgattcct tggcatgggg
    10501 cgttttgccc tttgcaagaa accttgtgcc cgaaagccgt tatcagcccg ttaaccctgc
    10561 ccccggcggc aagccagagg aagggcttat ggattggcaa gattgtggtg ccggggcagg
    10621 ggcgctggcc cccgcctgtt cagatcaggc cggccagcgg gctggaagga tcggcatatt
    10681 tgcgcgtggc catgcggccc gacagatagg cctcgcggcc cgcttccacc gccagcttca
    10741 tggcgcgggc catgcggatg gggtctttcg cctcggcgat ggcggtgttc atcagcacgc
    10801 cgtccacgcc cagctccatg gcgatggcgg catcgctggc ggtgccaacg ccggcatcca
    10861 ccagcaccgg caccttggcg ccttcgacga tgaggcggat cgtcacccgg ttctggatgc
    10921 caaggccaga gccgatcggc gcgcccagcg gcatgatcgc caccgcaccc gcctcttcca
    10981 gctgtttggc ggcaatcgga tcatccacgc aatagaccat gggcaggaag ccttccctgg
    11041 ccagcacttc ggtggccttc agcgtctcgc gcatgtcggg gtagagggtg cgcgcctcgc
    11101 ccagcacttc cagcttgacg agatcccagc cgcccgcctc gcgcgccagg cgcagggtgc
    11161 ggatggcatc gtcagccgtg aagcagccgg cggtgttggg caggtaggtg atcttcttgg
    11221 ggtcgatgaa gtcggtcagc atcggcgctg cgggatcggc gatgttgacc cggcgcaccg
    11281 ccacggtgac gatctcggcg cccgaggctt cgagagcggc ggcgttctgc gcgaaatcgc
    11341 ggtatttgcc ggtgccgacg atcaggcggc tgcggaaggt ccgcccggcc acgctccagc
    11401 tgtcatccgc gctcacctcg ccacctccca caaaatgcac gatttccaga atgtcgccgg
    11461 cgttgagcgc cacatcggcc agggtagagc gcggcacgat ggcgccattg cgttccactg
    11521 ccaccttggc ggggttgagg ccgatatcgg ccaccagctc ggccacggag gcaccggcgc
    11581 ggatacggcg cggctcgccg ttcaccgtca gcgaaattgc gtctgtcatg ccgggccaca
    11641 tggacgctgg ggcggccagc gtcaactcaa catggggcgc ggcgggttaa tgcgcgtggc
    11701 gcaggttgcg cagatgggcc atggccagca gcagcacgcc ggggatggtg aagccggctt
    11761 ccagcgggcc gtggggggcc agcacgccgg cgctcatcag ggcaaggccg gcaaggccca
    11821 gcagcagcag gcccggctgg cgatggcgcg cagcccccac gcccagcccc agcgcgccca
    11881 ccgccacggc cagcatcagc ccatattcat ggatgcgcgg atcaagcagg gcggtgccca
    11941 gggtgctggt ggccaaagcg ccggtgccga cggcagtggc gcccaggccc agcaccgcgg
    12001 ccagcgccag ccccgccacg caatgcaccg cgcaagcggc cgacagggcg atgcccaggc
    12061 gatcgacgcc gcggcgaatc agggaagggt gggcagccat ggcccgcatg tatgttacat
    12121 tataacattt cgcaaggggc gggcggctgg tcgtgcgcca gcttgccatc cgggcgccaa
    12181 ggcggcacac agcgcgccat gagcagcaag cgttccggca ccgttctttc cttcccccat
    12241 ggcgcggcgc ggcggcgcgg cttgccggcg ctcttggcgc tgtggctggc ggtggtggcc
    12301 gcgctggtgg tggcgatcgt ggtggtgggg ggcatcacgc ggctgaccga atcgggcctg
    12361 tcgatcaccg agtggaagcc tttgtcgggc attgtgccgc cgctgaacga ggcggcctgg
    12421 gcggccgaat ttgcccatta tcgccagatc gaccaatatg gcgcggtgca tgcgggcatg
    12481 acgctggccc agttcaaggg cattttcttc tgggaatata tccaccgcgt gctgggccgg
    12541 gtgattggca tggcggtgtt ggtgccgctg gtgtggtttg cctggcggcg gcacattcct
    12601 gccggctatg gctggcggct gctggcgctg ctggcgttga tcgggctgca gggcacgctg
    12661 ggctggctga tggtggcatc gggcctgaag ccggggatga cggaagtggc gccgctgtgg
    12721 ctgatggcgc atctgttgac ggcgctgttc acgctggccg ggctggtgtg gacggcgctg
    12781 gatctggtgg cgctggcggg ccatgcccgg gcgcggccgg cgcggctgac agggctgggc
    12841 ctgctggcgc tgctggggct gtgggtgcag ctgggctttg gcgcgctgat ggcgggcctg
    12901 cgcgccggcc gggtgacgga ccagtggccg ctgatgaacg gcagcttctg gcccggcgcc
    12961 acccagccgc gcgccagcct gctggcctat ctcaccgccg atccgcagat tgtccatttc
    13021 atccatcgct ggtgggcctg gggcgtggtg gcgctgctgg tggtgctggc gcggcgggtg
    13081 aaggcggccg gcgcgcgcgg ggcggcgttt gccatccatt gcgcctttgg cacgcaggtg
    13141 ctgctgggca ttgccacggt gatggcgggc gtgccggtgt ggctgggggt gttgcaccag
    13201 ctggtgggcg cgctgctggt ggtgagcgtg gtgtggggcg tgcatgcgct ggggcgccgg
    13261 ggatgaacgg gcagggggcc gcgctgatct ggtgcccctt tgccgatgag gcgagcgcgg
    13321 ccgcggtggc aggcgcgctg ctggacgaag ggctggtggc ctgcgtgaac attctgccgg
    13381 cggtgcgctc gctctatata tggcagggcg cgcgcggcga aggcgccgaa gtgggcgccc
    13441 tgttcaagac ccgcgccgat ctgctggagg ccgcgattgc ccggatcgcc gcgctgcatc
    13501 cttatgaggc gccggccgtg gtgggctggc attgcgatgg cgcggcagcg cccacgctgg
    13561 cctggctggg ggcgctgacg ggcggctgaa tgagcacggt attattttac cacctccgga
    13621 gcccgcggaa atcttgactc tgacggccat ttgcaggatt aggcgcgcca tctgcccgcc
    13681 ggcatccccg gcgggcttgt tattgaaaca ggaagccaag ccatgaaggc gctcaccaag
    13741 gtgacccagt cggccaagcc ggcagaggtc gagaagaagt ggcatcttat tgatgccgat
    13801 gggctggtgg tcggccgtgt tgccgtgatc atcgccaata tcctgcgcgg caagcacaag
    13861 cccagcttca ccccccatgt cgattgcggc gatcacgttg tggtgatcaa cgccgacaag
    13921 gtgaagttca ccggcaacaa gctgaagcag cagacctact acaagcacac cggctatgcc
    13981 ggcggcatca aggaagtgac cgccgacaag gtgctggctg gccgcttccc cgagcgcgtg
    14041 ctggaaaagg cggttgagcg catgatcccg cgcgggccgc tgggccgtgc gcagatgcgc
    14101 gccctgcacc tctacgccgg ggccgagcac ccgcatgccg gcaaccagcc cgaagtgctg
    14161 gatgtggccg cccgcaatcg caagaacaag gttggcgcgt aatgtctgaa gaattctccg
    14221 ctctcgccga tctcggcacc gatctgggcg cgcctgctgc tccttcggcc cctgctgccc
    14281 cgctgcgcga acgcgaagtc gatgcccagg gccgctccta tgccaccggc cgccgcaagg
    14341 atgccgtggc ccgcgtgtgg atcaagcccg gcaccggcaa gatcaccgtc aacggccgcg
    14401 accaggaagt gtatttcgca cgccccacgc tgcgcctggt gatcaaccag cccttcggca
    14461 ttgccgatcg ttcgggccag tatgatgtcg tctgcaccgt caagggcggc ggcctctcgg
    14521 gccaggctgg cgctgtgaag catggcatcg cccaggcgct cgccaagttc gagcctgccc
    14581 tgcgcagcac cgtgaaggcg gccggcttcc tcacccgcga cccgcgcgtg gtggaacgca
    14641 agaagtatgg ccgcgccaag gcccgccgga gcttccagtt cagcaagcgc tgatctggcc
    14701 ggattgcctt tggcatgcgg aagaaggggg cggcaacgcc cccttttttc gtatgtgcgc
    14761 cgcgcggcca tcgggcatgc gcggcggggc cgcggggctt tgccagcaga tgcggtatct
    14821 gacggagatt ttggaaaaat tttcaggggg tgattctggg ctctggcggt gcgggtgcat
    14881 ctgcggcgtg gtgaaggcct cattaaacct gtttaaaaac agtagtttgg ttgtgatctt
    14941 cataaaaatg caaaaagttg gttgaccgac tcgagggtgg ggcttagagg gcgcttcacc
    15001 gacgcggtga cgccgaaacg aggcgctact ggggaggtcg ccaagatgat tggatgctct
    15061 gagccctggc tctaaaggct gggagggatg agtgtcctgt taatttgttt tggtatgtta
    15121 gatctttgac attgttgttt ttgatgaagg gacatgtggg cgacggcgcc tgcgccttgg
    15181 ggtctttggg ctcgaggttg cagtttaaag taagccggtt gtctgtatgt ccttcgtatc
    15241 cattacgttt gacaa
//
LOCUS       DASRYE010000344         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_52602,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000344 DASRYE010000000
VERSION     DASRYE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52602"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFF98_18025"
     CDS             <1..1586
                     /locus_tag="VFF98_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZU65592.1"
                     /translation="GAAGGTAAVIAAAEGARVLVLEKMPIAGGTTAKSGGVCWIFNNF
                     VLREQGLVDAREDALKYAVRYGFPRQYDPASPTLGLDPVRFGTIAAFYDHGAPAIDRL
                     RELDAVQFKQFRMFAVNHPAPDYADHLPENKVPQGRCLEPAVGSGSGSGGGSLATQLR
                     GWLDKRGVPVLLNTRVTRILKDGTGRVVGVEAQQGGKTIRVKAARGVIFGSGGYSHNV
                     DLCNLHQPFVYGTCSLPGSTGDFIPMAEAAGARMGTLSYGWRSQVVLGEALASRGVGL
                     GAFVLPGDSMILVNKYGKRVVNEKRDYNDRTQAHFPYDPAREEYPNHLLFMLFDERSI
                     DAFGGAFPFPANKGEQPHLVEGATWEELSAKVDAQLAGWSGRTGGVRLAPTFSATTQD
                     TVARFNAYARAGKDPEQGRGDYLYDKEWHLLFSARREGTRFPANPYPNPVMHPFADKG
                     PYYCIILGPGTLDTAGGPQVNGHAQVLAADGTPIPGLYGAGNCISAPSGQAYLGAGGT
                     IGPALTFGYIAAKHAMGKA"
     gene            1583..1984
                     /locus_tag="VFF98_18030"
     CDS             1583..1984
                     /locus_tag="VFF98_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65593.1"
                     /translation="MKTLFTVISAAGLAALAPAAGAEAPAGSLFASKIAPIFASNCAV
                     CHLTGEEAGRMSLVPEQVIASTVNVPVNEAPGLTRIVPGKPDESYLVMKLEGTQVAHG
                     GTGARMPFGAAPLSPAEIALVRQWIREGAKP"
     gene            complement(2006..3220)
                     /locus_tag="VFF98_18035"
     CDS             complement(2006..3220)
                     /locus_tag="VFF98_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZU65594.1"
                     /translation="MPPARRDLLKAALAAPALLAGHAFAAPAAGGNRLLVVFLRGAYD
                     AANIIAPVASDFYHQARPTLALARPDPANADAALPLDADWGLHPALKDSILPLWQAGQ
                     IAFVPFAGTEDMSRSHFETQDTIELGQPLGATRDYGSGFMARLAGVLGASASAIAFTE
                     QLPLVFRGGAMVPNIALAGNGKPALDARQAGLVRQMYAGQHLAGVDLAAAVDTGFAVR
                     DTVYRTIAEEMTAAGRGAISARGFELAARRIGVLMRQTYNLAFVDVGGWDTHVNQGGA
                     QGYLAGRIGELGRALAGFAEEIGPAAWRTTTVVVVSEFGRTFRENGDKGTDHGHGSIY
                     WVLGGSVRGGRMAGPQVRIGADTLNQNRDLPVLTDYRALIGGLLARQYGLSPSQIARV
                     FPGAVPVDLGLV"
     gene            complement(3238..>4536)
                     /locus_tag="VFF98_18040"
     CDS             complement(3238..>4536)
                     /locus_tag="VFF98_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZU65595.1"
                     /translation="AAMRIQRENAADIAADMASQRRAINQIADPDAKKAADKAWQQAM
                     NDLAREAQTRAILRDLYSPFQLQEQMAWFWGNHFSVHARKREIRALLGDYDDALRAHA
                     LGRFADLLRASLTAPAMLQYLDNDQNAAGHINENYAREIMELHTLGVGSGYSQKDVQE
                     LARILTGVGVRLQGDGPRLAPNLQPLYIRRGLFEFNPARHDFGDKQFMGRTIRGQGFA
                     EVEQALDMLAHAPATARHVSAQIATYMLGQPPSPALVDSMASAWQHSDGRIDAVLQAL
                     FASAEYRASLGQGFKDPVHYVLSAVRYAYGDSTGPDSRVILNADPIIGWLSRMGEGLY
                     EHETPDGYPLAAAAWTGPGQMEIRFEIARAIGSGSAGLFRPRSAGSAEQPAFPQLQNA
                     LWFAQGPRLASAQTRAVLARATSPQEWNMLYLASPDFMRR"
BASE COUNT          707 a         1531 c         1591 g          707 t
ORIGIN      
        1 cgggcgcggc gggcggcacg gcggcggtga tcgccgcggc cgaaggcgcc cgggtgctgg
       61 tgctggaaaa gatgccgatc gccggcggca ccaccgccaa atcgggcggc gtgtgctgga
      121 tcttcaacaa tttcgtgctg cgcgaacagg ggcttgtcga tgcccgcgaa gatgcgctga
      181 aatatgccgt gcgctatggc tttccgcgcc agtatgatcc ggccagcccg acgctggggc
      241 tggatccggt gcgctttggc acgatcgcgg ccttttacga ccacggcgcg ccggcgatcg
      301 accggctgcg cgagctggat gcggtgcagt tcaagcagtt ccgcatgttt gccgtgaacc
      361 acccggcgcc cgattatgcc gaccacctgc ccgagaacaa ggtgccccag ggccgctgcc
      421 tggagccggc ggtgggttcg ggttcgggct cgggcggggg cagcctggcc acccagctgc
      481 gcggctggct cgacaagcgc ggcgtgccgg tgctgctgaa cacccgcgtc acccggatcc
      541 tcaaggatgg cactggccgc gtggtgggcg tggaagcgca gcagggcggc aagaccatcc
      601 gcgtgaaggc ggccaggggc gtgatcttcg gctcgggcgg ctattcgcac aatgtggatc
      661 tgtgcaacct gcaccagccc ttcgtctatg gcacctgctc gctgcccggc tccaccggtg
      721 atttcatccc catggcagag gcggccggcg cgcgcatggg cacgctgtcg tatggctggc
      781 gcagccaggt ggtgctgggc gaggcgctgg ccagccgcgg cgtggggctg ggcgcctttg
      841 tgctgccggg cgattcgatg atcctggtga acaaatacgg caagcgcgtc gtcaacgaga
      901 agcgcgacta taacgaccgc acccaggccc atttccccta tgatccggcg cgggaggaat
      961 atcccaacca cctgttgttc atgctgttcg acgagcgctc gatcgatgct tttggcggcg
     1021 ctttcccctt cccggccaac aagggcgagc agccccatct ggtggaaggg gcgacatggg
     1081 aggaactgtc cgccaaggtg gatgcccagc tggccggctg gagcgggcgc accggcggtg
     1141 tgcgcctggc ccccaccttc agcgcgacga cgcaggacac cgtggcccgc ttcaacgcct
     1201 atgcccgcgc cggcaaggat ccggaacagg gccgcggcga ttatctctac gacaaggagt
     1261 ggcacctgct gttcagcgcc cggcgcgagg gcacgcggtt cccggccaat ccctatccca
     1321 atccggtgat gcaccccttt gccgacaagg gcccctatta ctgcatcatc cttggccccg
     1381 gcacgctgga taccgccggc ggcccgcaag tgaacggcca tgcccaggtg ctggccgccg
     1441 atggcacgcc cattcccggc ctctatggcg cgggcaactg catctcggcg ccgtcgggcc
     1501 aggcctatct gggggccggc ggcacgatcg ggccggcgct cactttcggc tatatcgccg
     1561 ccaagcacgc gatgggcaag gcatgaagac gctgttcact gtcatcagcg cggcagggct
     1621 tgccgcgctg gccccggcag cgggggcaga ggcacccgcc ggctcgctgt tcgccagcaa
     1681 gatcgcgccg atcttcgcca gcaattgcgc cgtgtgccac ctcaccggcg aggaagccgg
     1741 gcgcatgtcg ctggtgcccg aacaggtgat cgcctccact gtcaacgtgc cggtgaacga
     1801 ggcgcccggg ctcacccgca tcgtgccggg caagcccgat gagagctatc tcgtgatgaa
     1861 gctggaaggc acgcaggtgg cccatggcgg caccggcgcg cgcatgccct ttggcgctgc
     1921 ccccctctcg ccggccgaga tcgcgcttgt ccgccagtgg atcagggaag gcgcgaagcc
     1981 ttgagatgag gcggcgccgg ccctctcaca ccaaccccag atccaccggc acggcgccgg
     2041 ggaacacgcg ggcgatctgg ctgggggaaa gtccgtattg ccgggccagc aacccgccga
     2101 tcagcgcgcg ataatcggtc agcaccggca ggtcgcggtt ctggttcagc gtgtcggcgc
     2161 cgatccgcac ctgcgggccg gccatgcgcc cgccccgcac gctgccgccc agcacccaat
     2221 agatgctgcc atggccatga tcggtgccct tgtcgccatt ctcgcggaag gtgcggccaa
     2281 attcggaaac gacgaccacc gttgtcgtgc gccatgcggc agggccgatc tcctcggcaa
     2341 agccggccag tgcccggccc agctcgccga tgcggccggc cagatagccc tgcgcgccgc
     2401 cctggttcac atgcgtgtcc cagccgccca catcgacgaa ggccaggttg taggtctggc
     2461 gcatcagcac gccgatgcgc cgcgcggcca gttcaaagcc gcgcgcgctg atcgccccgc
     2521 ggccggcggc ggtcatttcc tcggcgatgg tgcggtagac ggtgtcgcgc acggcaaagc
     2581 cggtgtcgac cgccgctgcc agatccaccc cggccagatg ctggccggca tacatctggc
     2641 gcacaaggcc ggcctgccgc gcatcgagcg ccggcttgcc attgccggcc agggcaatgt
     2701 tgggcaccat cgccccgccg cgaaagacca gcggcaattg ctcggtaaag gcgatggcgc
     2761 tggcgctggc gcccagcacg ccggccagcc gcgccatgaa gcccgaacca taatcgcgcg
     2821 tggcccccag tggctggccc agctcgatgg tgtcctgcgt ctcgaaatgg ctgcggctca
     2881 tgtcctcggt gccggcaaag ggcacgaagg cgatctggcc ggcctgccac agcggcagga
     2941 tgctgtcctt cagcgccggg tgcaggcccc agtcggcatc cagcggcagg gcggcatcgg
     3001 cattggccgg gtctggtcgg gccaatgcca gcgtggggcg ggcctgatgg tagaaatcgc
     3061 tggcgacagg ggcgatgatg ttagccgcat cataggcccc gcgcaagaac accaccagca
     3121 ggcggttgcc ccctgccgcg ggcgcggcaa aggcgtgacc ggccagcagg gcgggggcgg
     3181 cgagggcggc tttcagcaga tcgcggcggg cagggggcat catcgtctcc tcatcgctca
     3241 gcggcgcatg aaatcgggcg aggccaggta gagcatgttc cattcctgcg ggctggtggc
     3301 cctggccagc acggcgcggg tctgcgcgct ggccaggcgc ggcccctggg caaaccacag
     3361 ggcgttctgc aattgcggga aggcgggctg ttcggcgctg ccggcgctgc gcgggcggaa
     3421 caggccggcc gaaccgctgc cgatggcgcg ggcgatctcg aaacggatct ccatctggcc
     3481 gggccctgtc caggcggcgg cggccagcgg atagccatcg ggcgtctcgt gctcataaag
     3541 cccctcgccc atgcgcgaga gccagccgat gatcggatcg gcgttgagga tcacccggct
     3601 gtccggccct gtgctatcgc cataggcata gcgcaccgcc gacagcacat aatgcaccgg
     3661 atccttgaag ccctgcccca gcgaggcgcg atattccgcg ctggcaaaca gcgcctgcag
     3721 caccgcatcg atgcgcccgt cgctgtgctg ccaggcgctg gccatgctgt ccaccagcgc
     3781 tggcgagggg ggctggccca gcatgtaggt ggcgatctgc gccgagacat ggcgggcagt
     3841 ggccggcgca tgggccagca tgtcgagcgc ctgctccacc tcggcaaagc cctgcccgcg
     3901 gatggtgcgg cccatgaact gcttgtcgcc gaaatcgtgg cgggcggggt tgaactcgaa
     3961 caggccgcgg cggatgtaga gcggctggag gttgggcgcc aggcgcggcc catcgccttg
     4021 cagccgcacg ccaacgccgg tgaggatgcg ggccagctcc tgcacatcct tctggctgta
     4081 gcccgaaccc acccccagcg tgtgcagctc catgatctcg cgggcatagt tctcgttgat
     4141 gtggccggca gcgttctggt cgttgtcgag atattgcagc atggccggcg ctgtcaggct
     4201 ggcgcgcagc agatcggcaa agcggcccag cgcatgggcg cgcagggcat cgtcataatc
     4261 gcccagcagc gcgcggatct cgcgcttgcg ggcatggacg ctgaaatgat tgccccagaa
     4321 ccaggccatc tgctcctgca gctggaaggg ggaataaaga tcgcgcagga tggcgcgcgt
     4381 ctgcgcctcg cgggcaagat cgttcatcgc ctgctgccag gccttgtcgg cggccttctt
     4441 ggcgtcggga tcggcgatct ggttgatggc gcggcgctgg ctggccatgt cggcggcgat
     4501 gtcggcggcg ttttcgcgct ggatgcgcat ggccgc
//
LOCUS       DASRYE010000345         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_69961,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000345 DASRYE010000000
VERSION     DASRYE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69961"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..859
                     /locus_tag="VFF98_18045"
     CDS             35..859
                     /locus_tag="VFF98_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU65596.1"
                     /translation="MGQGLGPDVLAACRALFDEAQRALAAAVPPLATELAYGPDARHR
                     LDLYGEAGGALKPVLLFVHGGGFVQGDKGGPAEQWANAAVGRMAARQGWIGAVMNYRL
                     APDHGWPAGSEDVAAAIAFLRREGAAHGGDPARIFVMGTSAGAVHVAGFLRLRADHGD
                     LVRGAILLSGLYGYAPIDPRDERYYGPAATYADKVPLAAVASTGLPLLVACAQHDPAR
                     FQAEFLGLMQDRLARHGTMPRGVLLAGHNHYTMAMHLGTADRRLETEIASFLGEHA"
     gene            856..2190
                     /locus_tag="VFF98_18050"
     CDS             856..2190
                     /locus_tag="VFF98_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HZU65597.1"
                     /translation="MIDRRSMLAGASAAAALAASPVLARPAKGTAPVAPRAFPKGFLW
                     GAATAGHQVEGNNVNADVWVLEHVQPTIFSEPSGDACNSFALWATDLDLAKAMGLTAY
                     RFSLEWPRIEPEEGLFSIAMLDHYKAIIEGARARGLTPVVTFNHYTTPRWFATRGGWT
                     NPEAPSFFARFCEKAARHLAAHIGYATTLNEPNIMNVLRVILPPPVFQLQSAMLAAAA
                     KANACAKFAAGNAIDLPDINIATTHLIAGHKAGRAAIKAVRPDLPVGCSLSMFGDEAV
                     GANSVIGGMRAELYGPWLEAVRGDDFLGVQNYERVLWGSAGRVPAPKGAVLNRSGGEV
                     YAPSLAMAARYAHQVSGCPIMVTEHGVDTTDDTIRAALIPAALAELHKAMAEGLPVLG
                     YVHWSLIDNFEWISGMKGKLGLHAVDPVTFARTPKPSAGVLGAIARANAVTA"
     gene            2214..3113
                     /locus_tag="VFF98_18055"
     CDS             2214..3113
                     /locus_tag="VFF98_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZU65598.1"
                     /translation="MRKSLITAAFSLLASPALADMPAMVPAAPVADPAAIPLAPGMGS
                     LASEVWASAGGDMVVRNVTRPTLTPVLPAPGKATGAAVVVAPGGAFMLLAMDHEGWQV
                     AHWLADHGIAAFVLKYRLLPTPANPDAAAREMGARVMASVKDPSAPPALSDPAATEDA
                     LAALKLVRARAGQWGVDPARVGMIGFSAGAMTALNAALASDPAARPAFFGYVYGPQLA
                     VAVPAKAPPMFAAIAFDDSLFPNQGFAVETAWHKAGVPVELHAYQAGDHGFGTGKAGT
                     TSTGLLPQFRLWLEANGWLAAKP"
     gene            3218..3646
                     /locus_tag="VFF98_18060"
     CDS             3218..3646
                     /locus_tag="VFF98_18060"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HZU65599.1"
                     /translation="MPDAFPAQPAAGAGHALPPIPGTTLFDGDAAARGFALNAMCYSF
                     NEAANRAAFLADEDAYCARFGLTAPQRKAIADRDVLGMIAAGGNIYYLAKLAGIFHLN
                     VQDVGAMQTGQSVEAFKAMLQAQASGQTYEQAVEGSFAHG"
     gene            3639..4478
                     /locus_tag="VFF98_18065"
     CDS             3639..4478
                     /locus_tag="VFF98_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HZU65600.1"
                     /translation="MAEIVGAYFTSHVPSIGGAIHKGLQQDPYWKPFFDGYARVHEWL
                     AEVKPDVAIVFNNDHGLNFFLDKMPTFAVGAAPEYHNADEGWGLPLYKSFAGHVPLSW
                     HIIEQLVADEFDITTCQKMLVDHALSIPFELAWPDVDAWPVKIVPFAINTVQHPLPSA
                     KRCLALGQAVHRALQSWQGDERIVIIGTGGLSHQLDGERAGFMNRDYDLFCLDHLATD
                     PAALTKDSVHEIVRLAGTQGVEILNWIAARGALGEQAAEIHRNYHLPISNTAGATVLL
                     EPA"
BASE COUNT          683 a         1516 c         1539 g          747 t
ORIGIN      
        1 tgccagtgcc gccgccagtg ccgcgatcgc cgccatgggc caggggctgg gcccggatgt
       61 gctggcggcc tgccgcgcgc tgttcgacga ggcgcagcgc gcgcttgccg ctgccgtgcc
      121 gccgctggcg acggagctgg cctatggccc cgatgcccgc caccggctgg acctttatgg
      181 cgaggctggc ggggcgctga agccggtgct gctgttcgtg catggcggcg gctttgtcca
      241 gggcgacaag ggcgggccgg ccgagcaatg ggccaatgcc gcggtgggcc gcatggccgc
      301 gcgccagggc tggatcggcg cggtgatgaa ctatcgcctg gcgcccgatc atggctggcc
      361 ggccggcagc gaggatgttg ccgccgccat tgccttcctg cgccgcgaag gggcggccca
      421 tggcggcgat ccggcgcgga tcttcgtgat gggcacatcg gcgggcgcgg tgcatgtggc
      481 cggcttcctg cggctgcggg cggatcatgg cgatctggtg cgcggcgcca tcctgctttc
      541 cggcctctat ggctatgcgc cgatcgaccc gcgcgacgag cgctattatg gcccggccgc
      601 cacctatgcc gacaaggtgc cgctggccgc ggtggccagc accggcctgc cgctgctggt
      661 ggcctgcgcc cagcatgatc cggcgcgctt ccaggccgaa ttcctcggcc tcatgcaaga
      721 ccggctggcc cgccatggca ccatgccgcg cggcgtgctg ctggccggcc acaaccacta
      781 cacaatggcg atgcatctgg gcacggcaga ccgccggctg gaaaccgaaa tcgccagctt
      841 tctgggagaa catgcgtgat cgatcgtcgt tccatgctgg ccggggcctc ggccgcagcc
      901 gcgcttgccg ccagccccgt gctggcccgg ccggccaagg gcacagcgcc tgtggccccg
      961 cgcgccttcc ccaagggctt cctgtggggg gcggccactg ccggccatca ggtggaaggc
     1021 aataatgtca acgccgatgt ctgggtgctg gagcatgtgc agcccaccat cttttccgaa
     1081 cccagcggcg atgcctgcaa cagcttcgcc ctgtgggcga cagaccttga ccttgccaaa
     1141 gcgatgggcc tcaccgccta tcgcttcagc ctggaatggc cgcgcatcga gccggaggag
     1201 ggcctgttct ccatcgccat gctcgatcat tacaaggcga tcatcgaggg cgcccgcgcg
     1261 cgcggcctca ctcctgtcgt caccttcaac cattatacca ccccgcgctg gtttgccacg
     1321 cgcggcggct ggacgaaccc tgaggcgccc tccttcttcg cccgcttctg cgagaaggcg
     1381 gcgcggcatc tggccgccca tatcggctat gccaccacgc tgaacgagcc gaacatcatg
     1441 aacgtgctgc gggtgatcct gccgccgccg gtgttccagc tgcaatcggc gatgctggcg
     1501 gcggccgcca aggccaatgc ctgcgccaaa ttcgccgccg gcaatgcgat cgacctgccc
     1561 gatatcaaca ttgccaccac ccatctcatc gccggccaca aggcgggccg cgcggcgatc
     1621 aaggcggtgc ggccggatct gccggtgggc tgctcgctgt ccatgttcgg cgacgaggcg
     1681 gtgggggcca attcggtgat cggcggcatg cgggccgaac tgtatggccc gtggctggaa
     1741 gcggtgcgcg gcgacgattt cctcggcgtg cagaattacg agcgcgtgct gtggggttct
     1801 gccgggcgcg tgccggcgcc caagggggcc gtgctcaacc gctctggcgg cgaggtctat
     1861 gcgccctcgc tggcgatggc ggcgcgctat gcccatcagg tcagcggctg cccgatcatg
     1921 gtgacggaac atggcgtgga taccaccgac gacaccatcc gcgccgcgct catcccggcc
     1981 gcccttgccg aattgcacaa ggcgatggcc gaggggctgc cggtgctggg ctatgtccac
     2041 tggtcgctga tcgacaattt cgagtggatt tccggcatga agggcaagct gggcctgcat
     2101 gcggtcgatc cggtcacttt cgcgcgcacg cccaagccca gcgccggcgt gctgggggcc
     2161 attgcccggg ccaatgccgt gactgcctga agctggaaac ggggagaaga atgatgcgca
     2221 aaagcctgat caccgcggcc ttttccctgc tggccagccc ggcgctggcg gatatgccgg
     2281 caatggtgcc ggcagcaccc gtggccgatc cggcggcgat cccgctggcg cccggcatgg
     2341 gcagccttgc cagcgaggtg tgggccagcg ccgggggcga tatggtggtg cgcaatgtca
     2401 cccgccccac gttgaccccg gtgctgccgg cgcccggcaa ggccacgggc gctgccgtgg
     2461 tggtggcgcc gggcggggcc ttcatgctgc tggcgatgga tcatgaaggc tggcaggtgg
     2521 cccattggct ggccgatcat ggcattgccg ccttcgtgct gaaataccgc ctgctgccaa
     2581 cgccggccaa cccggatgcg gccgcgcgcg agatgggggc gcgggtgatg gcctcggtga
     2641 aggatccgtc ggcgccgccg gcgctgtccg atccggccgc caccgaggat gcgctggcgg
     2701 cgctgaagct ggtgcgcgcg cgggccgggc aatggggcgt ggatccggcg cgggtgggga
     2761 tgatcggctt ttccgccggg gcgatgacgg cgctgaatgc tgcgctcgcc agcgatccgg
     2821 cggcgcggcc ggcgtttttc ggctatgtct atgggccgca gctggccgtg gcggtgccag
     2881 ccaaggcgcc gccgatgttc gccgccatcg cttttgacga ttcgctgttc cccaaccagg
     2941 gctttgccgt ggaaaccgcc tggcacaagg cgggcgtgcc ggtggagctg catgcctatc
     3001 aggcgggcga tcatggcttt ggcacgggca aggcgggcac caccagcacc ggcctgctgc
     3061 cgcagttccg cctgtggctg gaggccaatg gctggctggc ggccaaacct tgagcactgg
     3121 caccatgcgc tctaatggat ccatttgacg ccgatcaagg acagatgaca ccataagcgg
     3181 gatgcatcag ggcaagagat ttgctctgga gagacagatg cccgatgcat tcccggccca
     3241 accggccgca ggggcaggcc atgccctgcc gccgatcccc ggcaccaccc tgttcgatgg
     3301 cgatgccgcc gccaggggtt ttgccctgaa cgccatgtgc tattccttca acgaggcggc
     3361 caaccgcgcc gctttcctcg ccgacgagga cgcctattgt gcgcgctttg gcctcaccgc
     3421 gccccagcgc aaggcgattg ccgatcgcga tgtgctgggc atgatcgcgg ccggcggcaa
     3481 catctactac ctcgccaagc tggccggcat cttccacctc aacgtgcagg atgtgggcgc
     3541 catgcagacc gggcagagcg tggaggcctt caaggccatg ctgcaggcgc aggccagcgg
     3601 gcaaacttat gaacaggccg ttgaagggag ctttgcacat ggctgaaatc gttggcgctt
     3661 atttcaccag ccatgtcccc tccatcggcg gggcgatcca caaggggctg cagcaggacc
     3721 cctactggaa gcccttcttc gacggctatg cccgcgtgca tgaatggctg gccgaggtga
     3781 agcccgatgt ggcgatcgtg ttcaacaacg atcacggcct caacttcttc ctcgacaaga
     3841 tgcccacctt tgccgtgggc gcggcccctg aataccacaa tgccgacgag ggctggggcc
     3901 tgccgctcta caagagtttt gccggccatg tgcccttgag ctggcacatc atcgagcaac
     3961 tggtggccga cgagttcgac atcaccacct gccagaagat gctggtggac catgcgctgt
     4021 cgatcccctt cgagctggcc tggcccgatg tcgatgcctg gccggtgaag atcgtgccct
     4081 ttgccatcaa caccgtgcag cacccgttgc cctcggccaa gcgctgcctg gcgctgggcc
     4141 aggcggtgca ccgcgcgctg cagagctggc agggggatga gcgcatcgtc atcatcggca
     4201 ccggcggcct gtcgcaccag ctggatggcg agcgcgccgg cttcatgaac cgcgactacg
     4261 acctcttctg cctcgatcac ctcgccaccg accccgccgc gctgacgaag gacagcgtgc
     4321 acgagatcgt gcgcctggcc ggcacgcaag gggtggagat cctcaactgg atcgccgcgc
     4381 gcggggcgct gggcgaacag gccgcggaaa tccaccgcaa ctatcacctg ccgatctcga
     4441 acacggccgg cgccaccgtg ctgctggaac cggcctgagg cggca
//
LOCUS       DASRYE010000346         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_52843,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000346 DASRYE010000000
VERSION     DASRYE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52843"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1387
                     /gene="serS"
                     /locus_tag="VFF98_18070"
     CDS             116..1387
                     /gene="serS"
                     /locus_tag="VFF98_18070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZU65601.1"
                     /translation="MHDIKLIRENPETFDAGLARRGLAPQAQAILALDSAAREAKLAF
                     ETRKAEANAANAEIGKAARSGDAAQVAALKEAAAAFKAELPALEAAVGEASAQVDAVL
                     AALPNLPADEVPEGPDEAANVEQKRWGDPRPAEGAKEHFDIGAPLGLDFESAQKVSGA
                     RFAYLRGQVARLNRALGQFMLDTHTTQFGYQEVAPPVLVRDEALFGTAQLPKFAEDLF
                     QTTDGRWLIPTAEVSLTNLVREAILADGDLPLRFTALTPCFRSEAGAAGRDTRGLIRQ
                     HQFDKVEMVSVTTPEQSEAEHERMTGAAEHILEKLGLPYRRMLLCSGDMGFSARKTYD
                     LEVWLPGQGTYREISSCSNCGDFQARRMGARYRPAGEKGTRFLHTLNGSGLAVGRTLV
                     AVLENWQNADGSVTVPPVLLPYMGGLTALRP"
     gene            1413..2177
                     /gene="surE"
                     /locus_tag="VFF98_18075"
     CDS             1413..2177
                     /gene="surE"
                     /locus_tag="VFF98_18075"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZU65602.1"
                     /translation="MRILLTNDDGINAPGLKVLEAIARQLSDDIWICAPAEEQSGAGH
                     SLTLTRPVRLRQHDERRFSVSGTPTDSVTMALRKVLPAAPDLILSGVNRGANLGDDVT
                     YSGTVSAAMEGALAGIRSIALSQVYAKEGVGDAVSFSAAQGWGEKVLRPLIEAPFAPR
                     TLININFPPIGAEAVQGIRVVRQGFHDYARGSVVEGTDPRGYKYFWFGLHGIEHTPGH
                     ATDLEAIADGFVSVSPLQLDLTHEGSLAMLAGRFTA"
BASE COUNT          354 a          792 c          810 g          365 t
ORIGIN      
        1 cgctcaccgg cacccgcctc agcgaggcgc gcgcgccggt gcaaatcacc gcctgaaccg
       61 cccatccgct ggctaatgct tgaccggcgc gcggcctgcc aataccgacc ttgccatgca
      121 cgatatcaaa ctcatccgcg agaatccgga aacgttcgat gccggccttg cccggcgcgg
      181 gctggccccg caggcgcagg cgattctggc gctggatagc gccgcgcgcg aggccaagct
      241 ggccttcgaa acccgcaagg ccgaggccaa tgccgccaat gccgagatcg gcaaggcggc
      301 gcgatcgggc gatgcggcgc aggtggccgc gctgaaggaa gcggcggccg ccttcaaggc
      361 cgaactgccg gcgctggaag cggccgtggg cgaggcatcg gcgcaggtgg acgcggtgct
      421 ggccgccctg cccaacctgc cggccgacga agtgcccgag ggccccgacg aggccgccaa
      481 tgtcgagcag aagcgctggg gggatcctcg ccccgccgag ggcgcgaagg agcatttcga
      541 tatcggcgcg ccgctgggcc tcgatttcga aagcgcgcag aaggtgtcgg gcgcgcgctt
      601 tgcctatctg cgcgggcagg tggcgcggct gaaccgggcg ctcggccagt tcatgctgga
      661 tacgcacacc acgcaattcg gctatcagga agtggcgccg cccgtgctgg tgcgcgatga
      721 agcgctgttt ggcaccgcgc agctgccgaa attcgccgag gatctgttcc agaccaccga
      781 tggccgctgg ctgatcccta ccgccgaggt cagcctcacc aacctcgtgc gcgaggcgat
      841 cctggccgat ggcgatctgc ccctgcgctt caccgccctc accccctgct tccgcagcga
      901 ggcgggcgcg gccgggcgcg acacgcgcgg cctgatccgc cagcaccagt tcgacaaggt
      961 ggagatggtc tctgtcacca cgcccgagca atcggaggcc gagcacgagc ggatgacggg
     1021 ggctgccgaa catattctgg aaaagctggg cctgccctat cgccgcatgc tgctgtgcag
     1081 cggtgacatg ggcttttccg cccgcaagac ctatgatctg gaagtgtggc tgccgggcca
     1141 gggcacctat cgcgagattt cctcctgctc caattgcggc gatttccagg cgcggcggat
     1201 gggcgcgcgc tatcgcccgg cgggcgagaa gggcacgcgc ttcctccaca cgctgaacgg
     1261 ctctggcctg gcggtgggcc gcacgctggt ggcggtgctg gaaaactggc agaatgccga
     1321 tgggtcggtc accgtgccgc cggtgctgct gccctatatg ggcgggctga ccgcgctgcg
     1381 cccctaacct cctcctgcca caaggttttt ccatgcgcat cctgcttacc aacgatgatg
     1441 gcatcaacgc ccccggcctc aaggtgctgg aggcgatcgc ccgccagctg tcggacgata
     1501 tctggatctg cgcgccggcc gaggaacagt cgggcgcggg ccacagcctc accctcacgc
     1561 ggccggtgcg cctgcgccag cacgacgagc ggcgcttctc ggtatccggc acgcccaccg
     1621 attcggtgac gatggcgctg cgcaaggtgc tgccggccgc gcccgatctc atcctctcgg
     1681 gcgtcaaccg cggcgctaac ctgggtgatg acgtgaccta ttcgggcacc gtttcggcgg
     1741 cgatggaagg ggcgctggcg ggcatccgct cgatcgcgct cagccaggtc tatgccaagg
     1801 aaggggtggg cgatgcggtc tccttcagcg cggcgcaagg ctggggcgag aaagtgctgc
     1861 gcccgctgat cgaggcgcct tttgcccccc gcacgctgat caacatcaac ttcccgccga
     1921 ttggcgccga agccgtgcag ggcatccgcg tggtgcgcca gggcttccac gattatgccc
     1981 gcggcagcgt ggtggaaggc accgatccgc gcggctacaa atatttctgg ttcggcctgc
     2041 atggcatcga gcacacgccc ggccatgcca ccgatctgga agcgattgcc gatggcttcg
     2101 tctcggtcag cccgctgcag ctcgatctca cgcatgaggg ctcgctcgcc atgctggccg
     2161 gccggttcac cgcgtgaggc ggcagggggc gtgtctgcgg cgctgtcgtc ggcggtggcg
     2221 gccggttccg ggcgggcctt ggcggggcgg gcgcctttgc gcggcggcgg gggcgcatcc
     2281 gccgcggctt gcctgggcag cagcgccggg atgatcaaca c
//
LOCUS       DASRYE010000347        25977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_52848,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000347 DASRYE010000000
VERSION     DASRYE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 25977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25977
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52848"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..848)
                     /locus_tag="VFF98_18080"
     CDS             complement(204..848)
                     /locus_tag="VFF98_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZU65603.1"
                     /translation="MLRAISPGHDAAPPARWQQRKSAATRQRLIDAGVIRLVEAGYGG
                     LTTAAVAERCKVSRGAMHHHFPTRMALVEAVVEHVFYQRMRGFLEAYFAAIGERGEDT
                     LVEIACDLHWQSVQSSEYAAYLELAVAARTDAELERCFTPAACRYDAVWTAEMIEAFP
                     QWRRHWEAMKLANDLVLTAHMGMLLHKPVFGEGERMERLRRYISHVLRALYEEA"
     gene            1126..3492
                     /locus_tag="VFF98_18085"
     CDS             1126..3492
                     /locus_tag="VFF98_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZU65604.1"
                     /translation="MTSSGTPRRGAHLRACALLAGLSLLALASTPALAEDAATTAPAT
                     TGPATTGIAEIVVTAQKKAENLQSVPISVAAVGGAVLANMAVTSLQGLQGSVPNVQID
                     NFANTPNSAVYSIRGIGVIEPDPYAGNTVSIVDDGVPQFFSMGALLDTYDVNRVEILR
                     GPQGTLFGANTTGGVVNVVSAQPKGEFGGYLKATGGNWNRFDINGAVELPIKKDLVSF
                     RVAGVHTQRDGWVTNIYNGKSMGDKDVSALRAQLLITPGSDLKITLQGEYVRGRNGAP
                     VVVNGGLPGEANYVAPGNYYNGAVLPTYTSPCANAGQPCTAPNQYYSGNNQVPDISNI
                     DTQFFVGTVNWNNTAIGNLTSISGYKHFVLFEYTDQDGTSKTNNATRRRTEGWQYSEE
                     LRDDFRVNEHITGTAGLFYMSTKYNHYQMYHLDFAAAGLAQLNQQFQSTESISAFAQT
                     YYKVTDRLKLSAGIRYTHDSVDARSTLDTGYNAGALDAANWAVVETDLNGNPRDLRNG
                     PHDIDVGGKKSWDNVGWKLSLDYTLGQNRMIYASWARGFKSGGYTGRIGVASDGDTPY
                     NPEKVDTFELGTKLDVIPRHLRFNLTGFYTSYRNMQVAEIYYDSATHVQGNRILNAAK
                     SRIFGFEAETQIVPTEGLTLRGSLAYLNARYQQFLYIDPVSGATVNLAGYDLQNAPHW
                     SGTIGANYTVKMANGGRLVTDIAWMYTAKKFYTAIEDTPRSTVQATSLVDGTLTYHTA
                     NDRYVLGLWVKNMFDKHYISTVYDSPGYMGLVGYAPPRQFGGSVAVNF"
     gene            3551..4663
                     /locus_tag="VFF98_18090"
     CDS             3551..4663
                     /locus_tag="VFF98_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65605.1"
                     /translation="MVCSPSAAQLHGLVARQHILDAIANHSRGVDRADAHLLGAAYHG
                     DATVDYGFFEGPAAQLVEILADAQKGALPTLHRTCHSALAVAGRHALAESYVIACVDG
                     GDVQRLVLGRYLDRLEEREGSWRIAKRTYVLDANCNRAGANPRLDPPLAYDHFTPVGG
                     KGAGDAGRVLLAHHFAATHSARKAAAMPSASPASDAPLPAAAALDAALSRDAIRQLVA
                     TYCRGVDRADAELLASIFWPDSSVISGVVNGSGPEFAEGIIAFITAHLDSCFHSVANE
                     WIEVTGDHGVGEHYVIAHMRMAGNEVLTGGRYIDRYERRAGIWKIAARSFVADWTQTQ
                     PSSHEDAGFYEALKNRGAFGRHDPVYAHWAALDPVA"
     gene            4660..5880
                     /locus_tag="VFF98_18095"
     CDS             4660..5880
                     /locus_tag="VFF98_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZU65606.1"
                     /translation="MSSPGAIATAARPSHVPAERVVDINIYDLPGQAADFHRAWQALQ
                     QGAPDIVWTQRNEGHWIALAGPIIAEVQGDHERFSNRVIIIPKSVGEQHGLIPTTIDP
                     PEHRPYRKLLNEGMSLAVVRGLHGQIRATAAALIDGFASAGHCDFTAQYAGELPIRIF
                     LEQVGIPLGDAGQLRLWAACMTRPGMPMSFEEAKAAFYAYLDPVVRARAADPGTDLIS
                     HVVSADIGGRRLSHEEALALATQILIAGVDTVVNILGFILLALARDPALRAAFAAMDS
                     RAVLAATNELFRRFGLVTIARTVRHDMVFHGVELKAGELVCIPTQLHGLDEAVNPAAL
                     VIDLARKGARHSTFGSGPHMCPGQELARAEVAITIEEWLKRIPDFRVAPDSDLSSGHG
                     IVGCITRLCLEWTP"
     gene            5929..6411
                     /locus_tag="VFF98_18100"
     CDS             5929..6411
                     /locus_tag="VFF98_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZU65607.1"
                     /translation="MTSPMPPTATCAGWTGWTPPLQRSAFHDDAHVDCGLFVGDADGF
                     VAFAQDMLAAMEATHHLLGQQRIEIHGPDQASGECYFQAWHGIRDEEGAPRDLFICGR
                     YIDEYTCKNGEWRISRRKLITDWVKNDPADHSFYAANPATNRPGRGGADFSQKRDWPC
                     "
     gene            6455..7777
                     /locus_tag="VFF98_18105"
     CDS             6455..7777
                     /locus_tag="VFF98_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU65608.1"
                     /translation="MTAEQLMSRYSPDFDVAVRGDKITADRYTTREWMDLEGKHLWPK
                     VWHLGGVLAELEEEGDFIRHNFGKDSIIMMRQADGSVKAFYNACPHRGNRLILGDSGS
                     VPRITCGYHGWQFSTDGTLVHVQDPDDFADGNPCGRVHLSELRCETWGPFVFWCMDDD
                     VPPLLEWLAPFPERLANYKLENWVRVLNLSCDANFNWKVVRDNFNESYHLPTIHPELA
                     TFINDGLPTTLFEMYETGHNSMWMIGHQATTRVDYHSAEVPAPLNDIARAWGINPEDY
                     NGHTGDIRAAVIKAKRELGPARGYTMYEGMSDQQLVDYFHCTLFPNLTLTMSPEQLQV
                     LRTEPHPTDPEKCIFQHWCLYPPIEGQKEIATPIGTVPFELDAVRRHSTYGDGVSLGF
                     VADQDLSIGTTQQQGLNSRGFKGCILPGQEKRIQRFHELLNDYVGAGK"
     gene            complement(7961..8740)
                     /locus_tag="VFF98_18110"
     CDS             complement(7961..8740)
                     /locus_tag="VFF98_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65609.1"
                     /translation="MTIALPDVVAVVTGAAGGIGRELVRQLKAAGATVVATDMAPAAE
                     IDGADHYLAHDVTSPAGWEAVRDLVAARYGRLDALVHNAGVSIVTKFEDTPLEQWHRL
                     NAVNVDSIVIGTQILLPLLKEGGKARKGGASVVNFSSVGGLRGAAFNAAYCTSKAAVK
                     MLSKCLGAEFAALGYNIRVNSVHPGGVDTPMLGSILDRYVELGAVANREVAQAAMEAR
                     HPIGRMGRPEEMGGGVVYLCSEAASFVTCAEFVIDGGFSQI"
     gene            complement(8752..9570)
                     /locus_tag="VFF98_18115"
     CDS             complement(8752..9570)
                     /locus_tag="VFF98_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65610.1"
                     /translation="MIELTAQRLKGRVAFVSGGLRGIGLACVERFLAEGAEVVLSDLD
                     AEDSEAVQQAIARLGQGASYVQANVAQEADWERASAFVKQRHGKLHILVNNAGIDLTG
                     PVETLSYASWQRIMSINVDGVFLGVKHFTPLLAESGAGVKGGASIINVSSIMGLVGYN
                     EVSAYNASKGAVRLFTKGIAIEFAQKRMPIRANSLHPGFVLTPLLNAGFQRWVDKGFA
                     EKPQDLVDTMAGTTPIGRLADPAELASAAFFLASDDSSYMTGAELVIDGGWTAQ"
     gene            complement(9567..10349)
                     /locus_tag="VFF98_18120"
     CDS             complement(9567..10349)
                     /locus_tag="VFF98_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZU65611.1"
                     /translation="MTRRLEGKVALVTGGTSGIGAGAVRRFAAEGAQVIFTGSKAEAA
                     AALCAETGARFVAHRVQDAAAWEALAAQISADYGRLDIAFANAGTEHGDSNVEDISIE
                     GWNSIIGVNLTGVMLTVQHAMRLMAANPGGAAGSIIVNSSMNAHRAMGNFMAYSVTKT
                     AVLALVKSAAVHSGNRGYKIRVNAVLPGVVETAMIANLIDQSGDPVATRAAYEGMSPM
                     RRMAKVEEVAGLVAYLASDDAAFISGGEYLIDGATTAGMNGV"
     gene            10530..11948
                     /locus_tag="VFF98_18125"
     CDS             10530..11948
                     /locus_tag="VFF98_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZU65612.1"
                     /translation="MAERDPELPGTPGRSPGISWSELMAIDSRPAPAILAEDHYEYLG
                     SDPIPVERYTSEAFAKAEREKMWPHVWQFAAREEDLPEPGDYIVFENAGRSWILSRQD
                     DGQIRAFHNVCLHRGRKLRQEDGAADQFTCPFHGFTWNKDGSFSHMPCSWDFKHLAAK
                     NLDLPEAEVESWAGYVFLREEKGGPSLAEFLAPLPDHFARWRHEECTTVIWVAKEVPA
                     NWKVVAEAFMEAWHTIVTHPQLLPFTGDCNAAYRNWGDHINVNLVPFGIMSPHLAGTD
                     KPQQWIVDEFVRYNGRSGDNYEAGEDPFAVTVPEGQSAREALGAAMRNAYTAQTGYDH
                     SDATDSELLDALVYNVFPNFAPWGGFMPNIVYRWLPGKTPDSCVMEVRILARGKKGEK
                     LPRSVPRKFLTSDQKWTDAPELGVLGDVFEQDMDNLPFVQEGLHASKNGEVQLADYQE
                     IRIRQFHRTMMKYLNGELGAQA"
     gene            11945..12703
                     /locus_tag="VFF98_18130"
     CDS             11945..12703
                     /locus_tag="VFF98_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZU65613.1"
                     /translation="MSEGQDEARPPRINAVLICGGVWHDMDFARLELLKLLAEDSSVR
                     TRVFEDYEHIEAITGADILITYTCNVVPSLEAQEALLEWLHKGGRWYALHGTNSVLRL
                     LENGLFDAPRWAPLFMETLGSQFITHPPIAPYRVTVADPTHPLVAGVEPFETTDELYH
                     LEQHGPLHVLLETECHVPGTGFVEAADAPGTHPVMYLKSHGQGAVLYNTLGHCRGHYD
                     LQPMLDWWPSVDRCAWDLPVFYDLLRRGIAWTKQ"
     gene            complement(12754..14388)
                     /locus_tag="VFF98_18135"
     CDS             complement(12754..14388)
                     /locus_tag="VFF98_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZU65614.1"
                     /translation="MFPQTDRAWDYIVVGAGSAGCVLAERLSASGRHHVLLLEAGGPN
                     DSFWVSLPKGVAKLVTNPAHIWAYPVAQPREGAGGAQEVWIRGKGLGGSSSVNGMIWS
                     RGEPADYDDWQAHGATGWNAASMTEAFLALEDHAAGPGPMRGTGGLVHVDPAIYTYPL
                     AETMIAAGEALGLQRVADLNATAGPRVGLYSHNIKAGRRQSSALTFLKAAAGRANLTV
                     VTGALAERIVFDENRRAVAVEARVNGQPARFALAGEVIVSSGTMESPLLLQRSGIGDG
                     ARLQAAGVAPVAHSPDVGERMIEHLSIAMPYRLNRGQGTNRHFYGLGAALAMARYLLR
                     HDGIMATGPFEVGAFCNVAHPDGRTDAQLYLGGYTFKVEDDGNPVPLDKIDPRPGITI
                     YGQLLRLTSESSIRIGGPTAADTPVILPNWLSTEHDRASAVAMMRKMRAYMAAGELGR
                     MVGEEEIPGKAVASDEELLAAFRRLSSCGLHGIRSCRMGEDERAVVDPRCRGQGVQGV
                     RVADCSIMPWHVTGNTNAPAMAVGLRAAAMVLEEAR"
     gene            14561..14956
                     /locus_tag="VFF98_18140"
     CDS             14561..14956
                     /locus_tag="VFF98_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZU65615.1"
                     /translation="MRPIAEGLFTEESPPRLIGARHRPSGRIVFPCPPGADYEPHPLG
                     RRGRLWSYTIQRYRPKSPPYTGPEAFKPWVVAYVELPGEVIVEARLDGVDFAALKIGM
                     EVEFAPVPLDPDAADSVLIPGFLPAGEAA"
     gene            14953..16101
                     /locus_tag="VFF98_18145"
     CDS             14953..16101
                     /locus_tag="VFF98_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZU65616.1"
                     /translation="MSDVCIIGAGIHPFGRTDGVSGLDQGVFAVRQALAEAGLEWRDV
                     QFAYGGSDAAGNPDTMVERLGLTGVQFINVRNGCAAGGSALFSAQMAIKSGEFDIGLA
                     VGFDKHPRGAFNALPAEYNLPDWYGEAGFMITTQFFAAKITRYMHLHGISRKTLGLVA
                     EKAFRNAVHAPHAWRRAPVPLDEILDGTMVSDPYSKYMFCSPGEGGVALLLASEKKAR
                     ELGITPIRLKAATMRTRPPHSFEVFAPMVDVGGGVATATRLASAAAFEMAGIGPADIA
                     VAQLQDTEAGAEIMHMAENGFCADGEQERWLAEGLTEIAGRLPVNTDGGCLACGEPIG
                     ASGLRQVYENVVQLRGAGGGRQVPGTPKTAYSHVYGAPGVSAVTILER"
     gene            16193..17176
                     /locus_tag="VFF98_18150"
     CDS             16193..17176
                     /locus_tag="VFF98_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZU65617.1"
                     /translation="MQLSREALLRAYRQMKVIREFEERLHVEIQTGEIAGFTHLYCGQ
                     EAVAVGVCEHLSAADKIISTHRGHGHCLAKGCDVDDMMKEIWGSREGLCKGKGGSMHI
                     ADVNVGMLGANGIVGAGAPIAVGAALALKLDGPDEAGLHVSLAFSGDGACNQGTTFEA
                     MNLAVVTKAPVIFVFENNHYSEHTGVDYAVGTAKDIASRAEAFGMKVWRADGIDFFSV
                     HDTMREVLEYVRAGNGPAAVEYDTERFFGHFEGDPQRYRGPGELDRIRETRDCLKQFR
                     KSVTAAKLLTDADLDALDTEVLEAIEAAVTAARAAERPTADDVTVDVYINY"
     gene            17194..18207
                     /locus_tag="VFF98_18155"
     CDS             17194..18207
                     /locus_tag="VFF98_18155"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZU65618.1"
                     /translation="MAKMMYREAVLSTIAEEMARDPSVVVLGEDVVGGMGTAGGPEAI
                     GGIWSTSTGLFGQYGAERVIDTPISESAIIGAASGLALAGKRPIAELMFADFIGVCLD
                     QIWNQMGKFRYMFGGKSRCPAVIRMAYGAGYNAAAQHSQSVHQILTGMPGLKVVMPSC
                     PADVKGLLRTAIRDDDPVIFLEHKALYAMKGEVPDDPDFTIPFGHARLTRAGQDVTIV
                     ASGLLLGFAEAAADKLAKDGIGCDVIDLRTTSPLDEESILDSVEVTGRLLVVDEAPPR
                     CGLAADICALVANKAFASLKAPPAACTPPHSPIPFARELETAYLPSADKIETAVRKLM
                     AWR"
     gene            18222..19679
                     /locus_tag="VFF98_18160"
     CDS             18222..19679
                     /locus_tag="VFF98_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZU65619.1"
                     /translation="MADIRPFCMPKWGIEMTEGTLAEWMVKEGDAFTRGQLLCLIETA
                     KITNEVEAEYDAVVKRVLVPSGGEPEPVGALLAVFAEAGASDAEVDAFIAAFKPADTA
                     VAAKTGGGAAEAPAPAAAPQPASPAPTAKAPIKIDTNRPISPEALKLAEAEAVDIAGI
                     EGSGRGGRITYQDVAQALRAAEGFAAPAPKGTAQLIEDNYKTFASPLARRIAALHAID
                     LTGVRGTGPRGRISKADVLALVPAPAPAASGLSSGPFVAVDNTPEVVPFDKIRKVVAQ
                     RLTQAKQELPHFYLRISARMDELIALRKTANLVLGAKASINDYIVMASARALARHPDV
                     NVQVHGQEIHRFPHADVAIAVASPKGLVTPIVRQADRMRIDQIAAATRALIDKAQAGK
                     LGYEDMAGGTFSVSNLGMFGIENFDAIINPPQGAILSVGTILRTPVETASGIAFESRI
                     SFTLSVDHRAIDGAAGAQFLATLKGLLEEPESLFA"
     gene            complement(19725..20804)
                     /gene="mtnA"
                     /locus_tag="VFF98_18165"
     CDS             complement(19725..20804)
                     /gene="mtnA"
                     /locus_tag="VFF98_18165"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZU65620.1"
                     /translation="MSGQRSPTRSRSIWREADGWSVGIFDQRRMPWAIETLRLTSAEE
                     AADAIRDMATRGAPLIGATAAYGLALALRAGADDAALARAVDLLAATRPTAVNLRWAL
                     ERVRARVAPLPPAARAAAAYAEADAICEEDEAVNRAIGAHGLPLIRALAAAKAPGEPV
                     NILTHCNPGWLATVAWGTATAPLYLAQDEGLALHVWVDETRPRNQGALTALELADHGV
                     PVSFIADNAGGLYMQRGLVDMVIVGTDRVAANGDVCNKIGTYLKALAARAHGVPFYVA
                     LPASTYDPATPSGDGIPIEERSGDELRFVSGPDEAGVMRKVAITAAPARNPAFDVTPA
                     ALVTGYITEKGVCAGPEALAAAFGG"
     gene            complement(20801..21502)
                     /gene="mtnC"
                     /locus_tag="VFF98_18170"
     CDS             complement(20801..21502)
                     /gene="mtnC"
                     /locus_tag="VFF98_18170"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HZU65621.1"
                     /translation="MSPVRAIVTDIEGTTSSIAFVAEVLFPYARARLAAYVAAHAQDL
                     APLLARVRAEAGDAGLDEQACVAQLLAWADADAKIGPLKELQGLIWAEGYADGTLKGH
                     IYADAAAGLARWQAAGLKLYVYSSGSVAAQKLLFGHSEAGDLTGLFAGWFDTGVGGKK
                     EAASYAAIARAIGETTAIAAAEILFLSDVPAELAAARAAGLQVILLDREGAAPAGDFA
                     VAVDFATILPAGAAA"
     gene            complement(21499..22053)
                     /locus_tag="VFF98_18175"
     CDS             complement(21499..22053)
                     /locus_tag="VFF98_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HZU65622.1"
                     /translation="MTHLAIYADQAPHAEIDSSADFGDISAVLAEAGIGLERWQAGAA
                     LGDAPADAAILAAYAPQVAALQARGGYRSVDVVRLTPDHPAAADMRAKFLAEHVHDDD
                     EVRFFVEGAGLFYIRHGGQVLALECTAGDLIVLPAGTRHWFDTGAAPRFTAIRLFTTP
                     EGWVARFTGDAIASAIPLYAGAAA"
     gene            complement(22050..22670)
                     /gene="mtnB"
                     /locus_tag="VFF98_18180"
     CDS             complement(22050..22670)
                     /gene="mtnB"
                     /locus_tag="VFF98_18180"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HZU65623.1"
                     /translation="MMYDEAAALICAAGRRLDARGLAPATAGNYSIRLEDGTLAVTVS
                     GAHKGRLTAAEIMRASATGEALEAKKPSAETLLHCLIYAVDGAAGAALHTHSIAGTVL
                     SRATAGAAILFTGYEVQKIFPGCATHDCTIAMPLVDNTQDMAALADQLRPIIAARTPI
                     LPAFYIRGHGLYAWGATMDAAENLVEGCEFLLACAWEELKLKGAGR"
     gene            complement(22667..23815)
                     /locus_tag="VFF98_18185"
     CDS             complement(22667..23815)
                     /locus_tag="VFF98_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HZU65624.1"
                     /translation="MTGSARKTTIFEHMSGLCRQLGAINLGQGFPEMGEPAELIAAAQ
                     RALAEHSNQYPPMRGLPQLRAAIAAYYGAAQGLAVDPAEVIVTSGATEALAASILALV
                     RPGDEVICVQPLYDAYVPLVRRAGGVVRLVSLRPPEWRLELADLAAAITPKTRLLILN
                     TPNNPTGTLLDRATLEGIGALCAEHGLVVLSDEVWEATLLDGAAPLSALAVDSLRQRT
                     VKVGSAGKIFSLTGWKLGWVVADPALAEEVAAIHQFLTFTTPTPLQWAVAEGLALPAP
                     WHARERARYAAARARLVEGLAGAGFAVLPNSGSWFVLVDLLASGLPAEDAALAEELVR
                     GGGVGSIPVSAFYEEAAQTGYLRLCFAKSADVLDEAVARLSQFRSNRA"
     gene            complement(23978..24391)
                     /gene="rplQ"
                     /locus_tag="VFF98_18190"
     CDS             complement(23978..24391)
                     /gene="rplQ"
                     /locus_tag="VFF98_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZU65625.1"
                     /translation="MRHKLGQRKLQRTSQHRTALLRNLSASLIKHEQITTTVPKAKEL
                     RPYIEKLITLAKRGGLSNRRLAHARLQDDAQLVKLFDVLAARYAARSGGYTRIIKAGI
                     RASDAAPIAVIELVDRDVAAKGQDSGPVLVAEDAE"
     gene            complement(24512..25570)
                     /locus_tag="VFF98_18195"
     CDS             complement(24512..25570)
                     /locus_tag="VFF98_18195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZU65626.1"
                     /translation="MTVNIKNWQELKKPNSLEIKPGTDPKRRATFVAEPLERGFGLTL
                     GNALRRVLLSSLQGAAITSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMQGEGP
                     KRLQLSATGPGVVKAGDIAVTGDIEVMNKDLVICHLDEGATLNMELTADTGKGYVPAV
                     SNRPADAPIGLIPVDSLYSPVRQVSYKVDNARIGQELDYDKLNLTVETDGTVTPEDAV
                     AYAARILQDQLALFVHFEDVAPIGVPAIAGLATAAPEEGDTNQLNRYLLKKVDELELS
                     VRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGW
                     PPENIEEMAKKLEQELLG"
     gene            complement(25683..>25977)
                     /gene="rpsK"
                     /locus_tag="VFF98_18200"
     CDS             complement(25683..>25977)
                     /gene="rpsK"
                     /locus_tag="VFF98_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZU65627.1"
                     /translation="TITDAQGNAISWSSAGMMGFKGSRKSTPYAAQVAADDAGKKAAE
                     HGVRTLEVEVKGPGSGRESALRALQAVGFTITSIRDVTPIPHNGVRPSKRRRV"
BASE COUNT         4465 a         9047 c         8239 g         4226 t
ORIGIN      
        1 gcccggatcg cggccgctca gcggctcggc cgggctggtg atgaggtcca tcgtcaatct
       61 ccccgccccg cctgtggcca gccggggagc ggcggcgcgg ggtctggccc aaggctgggc
      121 gcttggggct ttggggcaaa ctggcacttg tgagagattt caggcaggcc tgccggtttt
      181 gtgcgaatgc accagaaaac gcctcacgct tcctcgtaca gcgcccgcag cacatggctg
      241 atatagcggc gcaggcgctc catccgctca ccctcgccga acaccggctt gtgcagcagc
      301 atgcccatat gcgccgtcag caccagatca ttggccagct tcatcgcttc ccaatggcgg
      361 cgccactggg ggaaggcctc gatcatctca gccgtccaca ccgcatcgta gcggcaggcg
      421 gccggcgtga agcagcgctc cagctcggca tcggtgcgcg cggccacggc cagttccaga
      481 taggcggcat attcgctgga ctgcaccgat tgccagtgca gatcgcaggc gatttccacc
      541 agcgtatcct cgccccgctc gccaatggcg gcgaaatagg cttccagaaa gccgcgcatg
      601 cgctggtaga agacatgttc caccaccgcc tccaccagcg ccatgcgggt gggaaaatga
      661 tggtgcatgg ccccgcgcga aaccttgcag cgctcggcca cggcagcggt ggtgaggccg
      721 ccataaccgg cctccaccag gcggataacg cccgcatcga tcagccgctg gcgggtggca
      781 gcgctcttgc gctgctgcca gcgggccggc ggcgccgcat catgaccggg actgatcgcg
      841 cgcaacatgc gctgctgaac ggggtgagcc atagctttca acatcttaaa cgctgcgggc
      901 cgcaacgcaa gctgtggccc tgatcaaagt cgcagttacg cgcggctccg aatcgccgca
      961 aaaaaccggc aaggccgccg gttaaacgag cggccaggga gacgcaaccg gcaaggctgg
     1021 ccagccacag gcgccagcac cggcctgcgc ggccggcccc gccttagggc aggaaccggc
     1081 ttttgggtag ggcccgttgg gcccgaacag ggagaggagc gaatgatgac atcttcaggc
     1141 accccgcggc ggggggcaca cctgcgcgcc tgcgccttgc tggccgggct ttcattgctg
     1201 gcgctggcca gcacgccggc actggcggaa gacgccgcca ccacggctcc tgcgaccacg
     1261 gggcccgcca ccaccggcat tgccgaaatc gtggtgacag cccagaaaaa ggcggaaaac
     1321 ctgcaatccg tgccgatttc cgtggcggcc gtgggcggcg cggtgctggc caacatggcg
     1381 gttacctcgc tgcaggggct gcagggcagc gtgcccaacg tgcagatcga caatttcgcc
     1441 aacacgccca acagcgcggt ctattcgatc cgcggcatcg gcgtgatcga gcccgatccc
     1501 tatgccggca acaccgtgtc gatcgtcgac gatggcgtgc cgcagttctt ctcgatgggc
     1561 gccctgctcg acacctatga cgtcaaccgg gtggaaatcc tgcgcgggcc gcagggcacg
     1621 ctgtttggcg ccaacaccac aggcggcgtg gtgaacgtcg tctcggccca gcccaagggc
     1681 gaattcggcg gctatctcaa ggccaccggc ggcaactgga accggtttga catcaatggc
     1741 gcggtggagc tgccgatcaa gaaggacctt gtctccttcc gcgtggccgg cgtgcacacc
     1801 cagcgcgatg gctgggtgac gaacatctac aatggcaaga gcatgggcga caaggatgtc
     1861 tcggccctgc gcgcgcaatt gctgatcacg ccgggcagcg atctgaagat caccctgcag
     1921 ggcgaatatg tgcgcggccg caatggcgcg ccggtggtgg tgaacggcgg cctgccgggc
     1981 gaggccaatt acgtggcgcc gggcaattat tacaacggcg cggtgctgcc cacctatacc
     2041 agcccctgcg ccaatgccgg ccagccctgc acggcgccca accagtatta ttcgggcaac
     2101 aaccaggtgc ccgatatctc gaacatcgac acccagttct tcgtcggcac ggtgaactgg
     2161 aacaacaccg cgatcggcaa cctcacctcg atcagcggct acaagcattt cgttttgttt
     2221 gaatataccg accaggacgg cacctcgaag accaacaacg ccacccgccg ccgcaccgag
     2281 ggctggcaat acagcgagga actgcgcgac gatttccgcg tgaacgagca catcaccggc
     2341 accgccggcc tgttctacat gtcgaccaaa tacaaccact accagatgta tcacctggat
     2401 ttcgccgcgg ccgggctggc ccagctcaac cagcagttcc agagcaccga gagcatctcc
     2461 gccttcgccc agacctatta caaggtgacc gacaggctga agctttcggc cggcatccgc
     2521 tacacgcatg acagcgtcga tgcccgctcc accctcgaca ccggctacaa tgccggcgcg
     2581 ctcgatgccg ccaactgggc ggtggtggaa accgacctga acggcaatcc gcgcgatctg
     2641 cgcaatggcc cccacgatat cgacgtgggc ggcaagaaga gctgggacaa tgtcggctgg
     2701 aagctgagcc tggattacac gctgggccag aaccggatga tctatgccag ctgggcgcgc
     2761 ggcttcaaat cgggcggcta caccggccgc atcggcgtcg cctccgacgg tgatacgccc
     2821 tacaacccgg aaaaggtgga caccttcgag ctgggcacca agctggacgt gatcccgcgg
     2881 cacctgcgct tcaacctcac cggcttctac acctcctatc gcaacatgca ggtggccgag
     2941 atctactacg acagcgccac ccatgtgcag ggcaaccgca tcctcaacgc ggccaaatcg
     3001 cgcatcttcg gcttcgaggc ggaaacccag atcgtgccga ccgaagggct gaccctgcgc
     3061 ggatcgctgg cctatctcaa cgcccggtat cagcagttcc tctatatcga cccggtgagc
     3121 ggggcgacgg tgaacctggc cggctatgac ctgcagaacg cgccgcactg gtctggcacg
     3181 atcggcgcga attatactgt gaaaatggcc aatggcgggc gcctcgtcac cgacatagcc
     3241 tggatgtaca cggccaagaa gttctacacc gcgatcgagg acaccccgcg atcgaccgtg
     3301 caggccactt cgctggtcga tggcacgctc acctatcaca cggcgaacga ccgctatgtg
     3361 ctgggcctgt gggtgaagaa catgttcgac aagcactaca tctccaccgt ctatgattcg
     3421 cccggctaca tggggctggt gggctatgcc ccgccgcgcc agttcggcgg ctctgtcgct
     3481 gtcaatttct gatctgcccc gcctccgccg gccagctgcc ggcgggggct tttttgccaa
     3541 ggattgccag atggtttgct cccccagcgc cgcacagttg catggccttg tcgcccgcca
     3601 gcacatcctc gatgccatcg ccaaccacag ccgcggggtg gacagggccg atgcccatct
     3661 gctgggcgcg gcctatcatg gcgatgccac ggtggattac ggcttcttcg aagggcccgc
     3721 cgcgcagctg gtggagatcc tcgccgacgc gcagaagggg gccctgccca cgctgcaccg
     3781 cacctgccac agcgcccttg ccgtggccgg ccgccatgcc ctggccgaaa gctatgtgat
     3841 cgcctgcgtg gatggcggcg atgtgcagcg cctggtgctg ggccgctatc tcgaccggct
     3901 ggaagagcgc gagggcagct ggcgcatcgc caaacgcacc tatgtgctgg atgccaattg
     3961 caaccgcgcc ggcgccaatc cgcggctcga tccgccgctg gcctatgacc atttcacgcc
     4021 ggtgggcggc aagggggcgg gcgatgccgg ccgcgtgctg ctggcccatc atttcgccgc
     4081 cacccattcc gccagaaagg ccgccgccat gccctctgcc agccctgcct ccgatgcgcc
     4141 cctgcccgca gccgcagcgc ttgatgccgc cctctcgcgc gatgccatcc gccagctggt
     4201 ggccacctat tgccgcggcg tggacagggc cgatgccgag ctgctggcca gcatcttctg
     4261 gcccgattcg agcgtgatct cgggcgtcgt caacggctcc gggccggaat ttgccgaagg
     4321 gatcatcgcc ttcatcaccg cccatctcga cagctgcttc cattcggtgg ccaatgaatg
     4381 gatcgaggtg accggcgatc atggcgtggg cgagcattat gtgatcgccc atatgcgcat
     4441 ggccggcaat gaagtgctga ccggcggccg ctatatcgac cgctacgagc ggcgcgccgg
     4501 catctggaag atcgccgcgc gcagcttcgt ggccgactgg acgcagaccc agccttccag
     4561 ccacgaggat gccggcttct acgaagcgct gaagaaccgc ggtgcctttg gccggcacga
     4621 tccggtctat gcccattggg ccgcgctgga tccggtggcg tgagcagccc cggcgcgatc
     4681 gccaccgccg cccgcccttc gcatgtgccg gcggagcggg tggtggatat caacatctac
     4741 gacctgcccg gccaggccgc cgatttccac cgcgcctggc aggccctgca gcagggcgcg
     4801 cccgatatcg tgtggacaca gcgcaacgag ggccactgga tcgcccttgc cgggccgatc
     4861 atcgccgagg tgcagggcga tcacgagcgc ttttccaatc gcgtcatcat catccccaaa
     4921 tcggtgggag aacagcacgg cctcatcccc accacgatag atccgcccga acaccgcccc
     4981 tatcgcaagc tgctgaacga ggggatgagc cttgccgtgg tgcgcggcct gcacggccag
     5041 atccgcgcca ccgccgcggc cctgatcgac ggtttcgcca gcgccggcca ttgcgatttc
     5101 accgcccaat atgcaggcga gctgccgatc cgcatcttcc tcgagcaggt cggcattccc
     5161 ctgggcgatg ccggccagct gcgcctctgg gccgcctgca tgacccggcc cggcatgccg
     5221 atgagcttcg aggaggccaa ggccgccttc tatgcctatc tcgatccggt ggtgcgcgcc
     5281 cgcgcggccg atccgggcac agatctcatc agccatgtcg tcagcgccga tatcggcggg
     5341 cggcgcctgt cacatgagga agcgctggcg ctggccacgc agatcctgat cgccggggtg
     5401 gatacggtgg tcaacatcct cggcttcatc ctgctggcgc tggcgcgtga tccggccctg
     5461 cgcgccgctt ttgccgcaat ggacagccgc gcggtgctgg ccgccaccaa cgagctgttc
     5521 cgccgctttg gcctcgtcac catcgcccgc accgtgcgcc acgacatggt cttccacggg
     5581 gtggagctga aggcgggcga gctggtctgc atccccaccc agctgcacgg gctggacgag
     5641 gcggtgaacc ctgccgccct ggtcatagac ctggcccgca agggcgcgcg ccattccact
     5701 ttcggctctg gcccgcacat gtgccccggc caggaactgg cccgcgcgga agtggccatc
     5761 accatcgagg aatggctgaa gcgtatcccc gatttccggg tggcgcccga ttccgatctt
     5821 tccagcggcc acggcatcgt tggctgcatc acccgcctct gcctggagtg gaccccttga
     5881 gcaccccttt cgaccttgac gccaccttgc gtgattatgc cgcccgccgt gacatcgccg
     5941 atgccgccca ccgctacatg cgcgggctgg accggctgga cgccccccct gcagcgcagc
     6001 gccttccacg atgatgccca tgtcgattgc gggctgttcg tgggcgatgc cgatggtttc
     6061 gtggcctttg cccaggatat gctggccgcg atggaagcca cgcaccacct ccttggccag
     6121 cagcgcatcg agatccacgg gcccgatcag gccagcggcg aatgctattt ccaggcctgg
     6181 cacggcatcc gcgacgagga gggcgccccg cgcgacctgt tcatctgcgg gcgctacatc
     6241 gacgaatata cctgcaagaa tggcgagtgg aggatctcca ggcgcaagct tatcacagac
     6301 tgggtgaaga acgacccggc cgatcacagc ttctatgccg ccaaccccgc caccaaccgg
     6361 ccggggcgcg gtggcgccga tttcagccag aagcgcgatt ggccgtgctg aggccacgcc
     6421 gccttgggac cactttgaga acgaggatat caagatgacc gccgaacagc tgatgtcgcg
     6481 ctattcgccc gatttcgatg tcgccgtgcg tggcgacaag atcaccgccg atcgctacac
     6541 cacccgcgaa tggatggatc tggaaggcaa gcacctgtgg cccaaagtgt ggcacctggg
     6601 cggcgtgctg gccgagctgg aagaggaggg cgatttcatt cgccacaatt tcggcaagga
     6661 ttccatcatc atgatgcgcc aggccgatgg cagcgtgaag gccttctaca acgcctgccc
     6721 gcaccgcggc aaccggctga tcctgggcga ttcgggctct gtgcccagga tcacctgcgg
     6781 ctatcatggc tggcagttca gcaccgatgg cacgctggtg catgtgcagg atccggatga
     6841 tttcgccgat ggcaacccct gcggccgcgt ccatctctcc gaactgcgct gcgagacctg
     6901 gggccccttc gtgttctggt gcatggacga tgacgtgccg ccgctgctcg aatggctggc
     6961 ccccttcccc gagcgcctgg ccaactacaa gctggaaaac tgggtcaggg tgctgaacct
     7021 ctcgtgcgat gccaacttca actggaaggt ggtgcgcgac aatttcaacg agagctacca
     7081 cctgcccacc atccaccccg aactggccac cttcatcaac gacgggctgc ccaccaccct
     7141 gttcgagatg tatgagaccg gccacaattc catgtggatg atcggccacc aggccaccac
     7201 ccgcgtcgat tatcacagcg ccgaagtgcc cgccccgctg aacgatatcg cccgcgcctg
     7261 gggcatcaac ccggaagact acaacggcca caccggcgat atccgcgccg ccgtcatcaa
     7321 ggccaagcgc gaactggggc cagcccgcgg ctacaccatg tatgagggca tgagcgacca
     7381 gcagctggtg gattatttcc actgcacgct gttccccaac ctcaccctca ccatgtcgcc
     7441 cgagcagctg caggtgctgc gcaccgagcc gcacccgaca gacccggaaa agtgcatctt
     7501 ccagcactgg tgcctctatc cgcccatcga ggggcagaag gaaatcgcca ccccgatcgg
     7561 caccgtgccc ttcgagctgg atgccgtgcg ccgccacagc acctatggcg atggcgtgtc
     7621 gctgggcttc gtggccgatc aggatctgtc gatcggcacc acccagcagc aggggctcaa
     7681 ctcgcgcggg ttcaagggct gcatcctgcc gggccaggaa aagcgcatcc agcgcttcca
     7741 cgaactgctc aacgattatg tgggcgccgg caagtaagct ggcgtttacg ggatgaaaaa
     7801 ggagggctcg gccgatggcc gggccctttt tattattttg cagataaagg gaatgggagc
     7861 gcgagggtgt taaccctcgc atcagcccct tcccttctca aacacaaaaa gggcaggcag
     7921 gccaaagccc acccgccctt tttcccggct tcccgccaga tcagatttgg ctgaagcccc
     7981 cgtcgatgac gaattcggcg caagtgacga agctggccgc ttccgagcac agatagacca
     8041 cgccgccgcc catttcctcg ggccggccca tgcggccgat ggggtggcgc gcttccatcg
     8101 cggcctgggc cacttcgcgg ttggccaccg cgcccagctc cacatagcga tcgaggatcg
     8161 aacccagcat cggcgtatcc acgccgcccg gatgcaccga attcacccgg atgttatagc
     8221 ccagcgcggc aaattcggcg cccaggcact tgctcagcat cttcaccgcc gccttgctgg
     8281 tgcaataggc cgcgttgaaa gccgccccgc gcagcccccc cacgctggag aaattgacca
     8341 cgctggcgcc gcccttgcgg gccttgccgc cttccttcag cagcggcagc aggatctgcg
     8401 tgccgataac gatggaatcg acattcaccg cattcagccg gtgccactgt tccagcggcg
     8461 tatcctcgaa cttggtgacg atggagacgc cggcattgtg gaccagcgca tccagccggc
     8521 cataacgtgc agccaccaga tcgcgcaccg cttcccagcc ggccgggctg gtcacgtcat
     8581 gggcaaggta gtggtcggcg ccgtcgatct cggcggcagg ggccatgtcg gtggccacca
     8641 cggtggcgcc ggccgccttc agctggcgca ccagctcgcg gccgatgccg ccggcagcgc
     8701 cggtcaccac ggccaccaca tcgggaaggg caatcgtcat ctcatggctc cttattgcgc
     8761 ggtccagccg ccatcgatga ccagctcggc gccggtcatg taggagctgt cgtcgctggc
     8821 gaggaagaaa gcggcgctgg ccagttcggc cgggtctgcc aggcggccga tcggcgttgt
     8881 cccggccatc gtgtcgacca gatcctgcgg cttttcggca aagcccttgt ccacccagcg
     8941 ctggaagccg gcattgagca gcggcgtcag cacaaagccg gggtgcagcg aattggcgcg
     9001 gatcggcatc cgcttctggg caaattcgat ggcgatgccc ttggtgaaca ggcgcaccgc
     9061 ccccttgctg gcgttatagg ccgacacctc gttatagccc accagcccca tgatcgagga
     9121 gacgttgatg atggaagcgc cgcccttcac gcccgcgccg ctctcggcca gcagcggggt
     9181 gaaatgcttg acgcccagga acacgccatc aacgttgatg ctcatgatcc gctgccagct
     9241 ggcatagctc agcgtttcca caggaccggt aaggtcgatg ccggcattgt tgacgagaat
     9301 gtgcagcttg ccatggcgct gcttcacaaa ggccgaggcg cgctcccaat cggcttcctg
     9361 ggccacattg gcctgcacat agctggcgcc ctggcccagc cgggcgatcg cctgctgcac
     9421 cgcctcgctg tcctcggcat caaggtcgga gagtaccacc tcggcgccct cggccagaaa
     9481 ccgctccaca caggcaaggc caatgccgcg cagccccccc gaaacaaatg ccacgcggcc
     9541 cttcagccgc tgcgccgtca gctcgatcat accccgttca tccctgcggt tgttgcgccg
     9601 tcgatcagat attcgccgcc cgaaatgaag gcggcatcat cgctggcgag ataagccacc
     9661 agcccggcca cttcctccac cttggccatg cgccgcatcg ggctcatgcc ctcgtaagcg
     9721 gcgcgcgtgg ctaccggatc gcccgactgg tcgatgagat tggcgatcat cgctgtctcg
     9781 acaacgccgg gcagcaccgc attcacgcgg atcttgtagc cgcggttgcc gctgtgcacg
     9841 gccgccgact tgaccagcgc cagcaccgcc gtcttggtga cggaataggc catgaaattg
     9901 cccatcgccc gatgcgcgtt catcgaggaa ttgacgatga tcgagccggc agcgccgccc
     9961 gggtttgccg ccatcagccg catggcatgc tgcacggtga gcatgacccc ggtgaggttg
    10021 acgccgatga tgctgttcca gccctcgatc gagatgtctt ccacattcga atcgccatgt
    10081 tcggtgccgg cattggcaaa ggcgatgtcc agccggccgt aatcggcgct gatctgcgcg
    10141 gccagcgctt cccaggcggc cgcatcctgc acccggtgcg ccacgaagcg cgcgcccgtc
    10201 tcggcgcaca gggccgcggc cgcttccgcc ttcgatccgg tgaagatcac ctgcgcgccc
    10261 tctgcggcaa accgccgcac agcgcccgcg ccaatccccg acgtgccgcc ggtgaccagc
    10321 gccacctttc cttcgagccg tctcgtcacg gcgctctccc ttatgtgttg cgtgcgaggg
    10381 gcgccccaaa agcgcccgca ttgccttgat ggatcgtctg aaccatattc ggaagcgcgt
    10441 aacctatgat ttgcgcaagg tgacgcccgc tgccggcaag ggcaaaaatc cgctggcacg
    10501 taacaatatt gggatgaagg acaaggatca tggcagagcg cgacccagag cttccgggca
    10561 cccccggccg cagcccgggc atcagctgga gcgagctgat ggcgatcgat tcccggcccg
    10621 cgccggccat cctcgccgag gatcactacg aatatctcgg ctccgacccg atccctgtgg
    10681 aacgctacac cagcgaggcc ttcgccaagg ccgagcgcga gaagatgtgg ccccatgtgt
    10741 ggcagtttgc cgcgcgcgag gaagacctgc cggaaccggg cgattacatc gtcttcgaga
    10801 atgccggccg ctcgtggatc ctctcgcgcc aggatgatgg ccagatccgc gccttccaca
    10861 atgtgtgcct gcatcgcggc cgcaagctgc gccaggaaga tggcgcggcc gatcagttca
    10921 cctgcccctt ccacggcttc acctggaaca aggatggcag cttcagccac atgccctgct
    10981 cgtgggattt caagcatctg gctgccaaga acctcgatct gccggaagcc gaggtggaga
    11041 gctgggcggg ctatgtgttc ctgcgcgagg aaaagggcgg cccctcgctg gccgagttcc
    11101 tggcccccct gcccgaccat ttcgcccgct ggcgccacga ggaatgcacc acggtgatct
    11161 gggtggccaa ggaagtgccg gccaactgga aggtggtggc cgaggccttc atggaagcct
    11221 ggcacaccat cgtcacccac ccgcagctgc tgcccttcac cggcgattgc aacgcggcct
    11281 atcgcaactg gggcgatcac atcaacgtca acctggtgcc cttcggcatc atgtcgccgc
    11341 atctggccgg caccgacaag ccacagcagt ggatcgtcga cgaattcgtg cgctataacg
    11401 gccgctcggg cgacaattac gaggccggcg aggatccctt cgccgttacc gtgccggaag
    11461 ggcagagcgc ccgcgaggcg ctgggcgcgg ccatgcgcaa cgcctatacc gcccagaccg
    11521 gctatgacca cagcgatgcc accgacagcg agctgctcga cgcgctggtc tacaatgtct
    11581 tccccaattt cgcgccctgg ggcggcttca tgccgaacat cgtctatcgc tggctgcccg
    11641 gcaaaacgcc cgacagctgc gtgatggaag tgcgcattct ggcccgcggc aagaagggcg
    11701 agaagctgcc gcgcagcgtg ccccgcaagt tcctcaccag cgaccagaag tggaccgatg
    11761 cgcccgaact gggcgtgctg ggcgatgtgt tcgagcagga tatggacaac ctgcccttcg
    11821 tgcaggaagg cctccacgct tccaagaacg gcgaagtgca gctggccgat tatcaggaaa
    11881 tccgcatccg ccagttccac cgcacgatga tgaaatatct gaacggcgag ctgggagccc
    11941 aggcgtgagc gagggacagg acgaagcccg cccgccgcgc atcaatgcgg tgctgatctg
    12001 cggtggggtg tggcacgaca tggatttcgc ccggctggag ctgctgaagc tgctggccga
    12061 ggacagcagc gtgcgcaccc gcgttttcga ggattacgaa catatcgagg cgatcaccgg
    12121 cgccgacatc ctgatcacct acacctgcaa tgtggtgccc agcctggaag cgcaggaagc
    12181 gctgctggaa tggctgcaca agggcgggcg ctggtatgcg ctgcatggca ccaattcggt
    12241 gctgcgcctg ctggaaaacg gcctgttcga tgcgccgcgc tgggcgcccc tgttcatgga
    12301 gacgctgggc agccagttca tcacccatcc gcccatcgcc ccctaccggg tgaccgtggc
    12361 cgatcccacc cacccgctgg tggccggtgt cgagccgttc gagacgaccg acgagctcta
    12421 tcatctggaa cagcacgggc cgctgcatgt gctgctggaa accgaatgcc acgtgccggg
    12481 caccggcttt gtggaagcgg ccgatgcgcc gggcacacat ccggtcatgt atctcaaatc
    12541 gcacgggcag ggcgcggtgc tctacaacac gctgggccat tgccgcggcc actacgacct
    12601 gcagccgatg ctcgactggt ggcccagcgt ggatcgctgc gcctgggatc tgccggtgtt
    12661 ctacgatctg ctgcgccggg gcatcgcctg gacgaagcag taaagcggca ggaaaggcgc
    12721 gaggggttaa gccccccccg cgcctttccc ccttcagcgc gcctcttcca gcaccatcgc
    12781 cgcggcccgc aggcccacgg ccatcgccgg cgcattggtg ttgccggtca catgccaggg
    12841 catgatcgag caatccgcca cgcgcacccc ctgcacgccc tgcccccggc agcgcggatc
    12901 gaccacggcg cgctcgtcct cgcccatgcg gcaggagcgg atgccatgca gcccgcagct
    12961 cgacaggcgg cggaaggcgg ccagcagttc ctcgtcgctt gccaccgcct tgccggggat
    13021 ctcctcctcg cccaccatgc ggcccagctc gcccgcggcc atataggcgc gcatcttgcg
    13081 catcatcgcc acggccgagg cccggtcatg ctcggtagac agccagttgg gcaggatcac
    13141 cggcgtgtcg gccgcggtgg gcccgccgat gcggatgctg ctctcgctgg tcaggcgcag
    13201 cagctggcca tagatggtga tgcccgggcg cgggtcgatc ttgtcgagcg gcaccgggtt
    13261 gccatcatcc tccaccttga aggtatagcc gcccagatag agctgcgcat cggtgcgccc
    13321 atccggatgc gccacattgc agaatgcgcc cacctcgaag ggcccggtgg ccatgatgcc
    13381 atcgtggcgc agcagatagc gcgccatcgc cagcgccgcg cccagcccat agaaatggcg
    13441 gttggtgccc tgcccccggt tcagccgata gggcatggcg atggaaaggt gctcgatcat
    13501 ccgctcgccc acatcgggcg aatgggccac cggcgccacg cccgccgcct gcagccgcgc
    13561 gccatcgcca atgccggagc gctggagcag cagcgggctt tccatcgtgc ccgacgagac
    13621 gatcacctcg ccggccagcg caaagcgggc gggctggccg ttcacccgcg cttccacggc
    13681 caccgcgcgg cggttttcgt caaacacgat ccgctcggcc agcgcgccgg tgacgacggt
    13741 gaggtttgcc cgccccgcgg ccgccttcag gaaggtgagc gcgctgctct ggcggcggcc
    13801 ggccttgatg ttgtggctgt agagccccac gcgcgggccc gccgtggcgt tgagatcagc
    13861 cacgcgctgc agccccagcg cctcgccggc agcgatcatc gtctcggcca gcggataggt
    13921 gtagatcgcc ggatcgacat ggacgagccc gcctgtgcca cgcatggggc ccggccccgc
    13981 ggcatggtcc tccagcgcga ggaaggcttc tgtcatgctg gccgcgttcc agccggtggc
    14041 gccatgggcc tgccagtcgt cataatcggc cggctccccc cggctccaga tcatgccgtt
    14101 gaccgaggag gaaccgccca gccccttgcc cctgatccac acttcctgcg cgccgcccgc
    14161 cccttcgcgc ggctgcgcca ccggataggc ccagatatgc gccgggttcg tcaccagctt
    14221 ggccacgccc ttgggcaggc tcacccagaa gctgtcgttg gggccgcctg cttccagcag
    14281 cagcacatga tggcggccag aggcgctcaa cctttcggcc agcacgcagc cggccgatcc
    14341 tgcccccacc acgatatagt cccaggctct gtcggtttgt ggaaacatgt tcgattatcc
    14401 tttcccgact tggcttttct catagctgcc gcccggcccc accctagcgt tttggggagt
    14461 cgctaagctg gcgcctgaag ccccagaaag caggctcaag cggaatcaca aaggccgccg
    14521 ccggcagcgc cggcccggcc cagacaaacg ggaaagaggc atgcggccca tcgccgaagg
    14581 attgtttacc gaggagagcc cgccacggct gatcggcgcg cgccatcgcc cctcggggcg
    14641 gatcgtgttc ccctgcccgc ccggcgccga ttacgagccc cacccgctgg gccgccgcgg
    14701 ccggctgtgg agctacacca tccagcgcta tcgccccaag agcccgccct acaccgggcc
    14761 cgaggcgttc aagccctggg tggtggccta tgtggagctg cccggcgagg tgatcgtcga
    14821 ggcaaggctc gatggcgtcg atttcgcagc tctcaagatc ggcatggaag tggaatttgc
    14881 ccccgtgccg ctcgatcccg atgccgccga cagcgtgctg atccccggct tccttcccgc
    14941 aggagaagcc gcatgagcga tgtgtgcatc attggcgccg gcatccaccc gtttggccgc
    15001 acagatggcg tctcggggct ggatcagggc gtgtttgccg tgcgccaggc gctggccgag
    15061 gccgggctgg aatggcggga cgtgcaattc gcctatggcg gatcggatgc cgccggcaac
    15121 cccgacacga tggtggagcg gctggggctg accggcgtgc agttcatcaa tgtgcgcaat
    15181 ggctgcgcgg cgggtggatc ggcgctgttc tcggcgcaga tggcgatcaa gtcgggcgag
    15241 ttcgacattg gcctggccgt cggcttcgac aagcacccgc gcggcgcctt caacgccctg
    15301 ccggccgaat acaacctgcc cgactggtat ggcgaggcgg gcttcatgat caccacgcaa
    15361 ttcttcgccg ccaagatcac ccgctatatg cacctgcacg gcatcagccg caaaacgctg
    15421 gggctggtgg cggaaaaggc cttccgcaat gcggtgcacg ccccccatgc ctggcggcgc
    15481 gcgccagtgc cgctcgacga gatcctcgac ggcacgatgg tctccgatcc ctattccaaa
    15541 tacatgttct gctcgcccgg cgagggcggc gtggcgctgc tgctggccag tgagaagaaa
    15601 gcgcgcgagc tgggcatcac ccccatccgc ctgaaggcgg ccaccatgcg cacccgcccg
    15661 ccgcacagtt tcgaggtgtt tgcccccatg gtcgatgtcg gcggcggggt ggcgaccgcc
    15721 acccggctgg cctcggccgc cgccttcgag atggccggca ttggcccggc cgacatcgcg
    15781 gttgcccagc tgcaggacac cgaggccggc gccgagatca tgcatatggc cgagaacggc
    15841 ttttgcgccg atggcgagca ggaacgctgg ctggccgaag ggctgaccga gattgccggc
    15901 cgcctgccgg tgaacaccga tggcggctgc cttgcctgcg gcgagccgat cggcgcctcg
    15961 ggcctcaggc aggtctacga gaatgtcgtg cagctgcgcg gcgccggtgg cggccgccag
    16021 gtgcccggca ccccgaagac cgcctacagc catgtctatg gcgcgccggg ggtctcggcc
    16081 gtcaccattc tggaacgcta ggcgtgatcc gcctcgccaa agccaaaccc ttgttttatc
    16141 gtgcccaggc tgcccgccag ccaaagacac ttgtcaaaga gaggaatgcc acatgcagct
    16201 gagccgcgaa gcgctgctgc gcgcctatcg ccagatgaag gtgatccgcg agttcgagga
    16261 gcgcctgcac gttgagatcc agaccggcga gatcgccggc ttcacccacc tctattgcgg
    16321 ccaggaagcc gtcgctgtgg gggtgtgcga acatctctcc gccgccgaca agatcatctc
    16381 cacccatcgc ggccacggcc attgcctggc caagggctgc gatgtggacg acatgatgaa
    16441 ggaaatctgg ggcagccgcg aggggctctg caagggcaag ggcggctcca tgcacatcgc
    16501 cgatgtgaat gtgggcatgc tgggggccaa tggcatcgtt ggcgccggcg cgccgatcgc
    16561 cgtgggcgcg gcgctggcgc tcaagctgga tggcccggac gaggcagggc tccatgtctc
    16621 gcttgccttc tcgggcgatg gcgcctgcaa ccagggcacc accttcgagg cgatgaacct
    16681 tgccgtggtg accaaggccc cggtgatctt cgtcttcgag aacaaccatt attccgagca
    16741 taccggcgtt gattacgcgg tcggcacggc gaaggacatc gccagtcgcg ccgaggcctt
    16801 cggcatgaag gtgtggcggg ccgacgggat cgatttcttc tcggttcacg acaccatgcg
    16861 cgaagtgctg gaatatgtgc gcgccggcaa cggcccggcc gcggtggaat atgacaccga
    16921 gcgcttcttc ggccatttcg agggcgatcc gcagcgctat cgcggccccg gcgaactcga
    16981 ccgcatccgc gagacgcgcg attgcctcaa gcagttccgc aaaagcgtga ccgcggccaa
    17041 gctgctgacc gatgccgacc tcgacgcgct cgataccgaa gtgctggaag ccatcgaagc
    17101 tgccgttacc gccgcccgcg ccgccgagcg ccccaccgcc gacgatgtca ccgtcgacgt
    17161 ttatatcaat tattgaggcg aggaggcaga gccatggcaa aaatgatgta ccgcgaggcc
    17221 gtcctctcga cgatcgctga ggaaatggcg cgcgatccca gtgtggtcgt gctgggtgag
    17281 gatgtggtgg gcggcatggg caccgccggt ggccccgagg cgatcggcgg catctggtcc
    17341 acttccaccg gcctgttcgg ccagtatggc gccgaacgcg tgatcgacac gccgatttcg
    17401 gaaagcgcca tcatcggcgc ggcctctggc ctggcgctgg ccggcaagcg gccgatcgcc
    17461 gagctgatgt ttgccgattt catcggcgtg tgccttgacc agatctggaa ccagatgggc
    17521 aagttccgct acatgttcgg cggcaagtcg cgctgcccgg cggtgatccg catggcctat
    17581 ggcgcgggct ataacgcggc ggcccagcac agccagtcgg tgcaccagat cctcaccggc
    17641 atgcccggcc tcaaggtggt gatgccatcc tgcccggccg atgtgaaggg cctgctgcgc
    17701 accgccatcc gcgatgatga tccggtgatc ttcctggagc acaaggcgct ctatgccatg
    17761 aagggcgaag tgccggacga tccggatttt accattccct tcggccatgc ccgcctcacc
    17821 cgcgccggcc aggatgtgac gatcgtcgcc tcgggcctgc tgctgggctt tgccgaagcg
    17881 gcggccgaca agctggccaa ggacggcatc ggctgcgatg tgatcgacct gcgcacgacc
    17941 agcccgctcg acgaggaaag catcctcgac agcgtggaag tgaccggccg cctgctggtg
    18001 gtggacgagg cgccgccgcg ctgtggcctt gcggccgata tctgcgcgct ggtggccaac
    18061 aaggcctttg ccagcctgaa ggcgccgccg gccgcctgca ccccgcccca ttcgcccatt
    18121 ccgttcgccc gtgagctgga aaccgcctat ctgccctcgg ccgacaagat cgagacagcg
    18181 gtgcgcaagc tcatggcctg gcgctgaagg aggcccgcat catggccgat atccgtccct
    18241 tctgcatgcc caagtggggc atcgaaatga ccgagggcac gcttgccgaa tggatggtga
    18301 aggaaggcga tgccttcacc cgcggccagc tgctgtgcct gatcgaaacc gccaagatca
    18361 ccaatgaggt ggaagccgaa tatgacgcgg tggtgaagcg tgtgctggtg ccctccggcg
    18421 gcgagccgga accggtgggc gcgctgctgg ccgtgtttgc cgaggccggc gccagcgatg
    18481 ccgaggtgga tgccttcatc gccgccttca agccggccga caccgccgtt gccgccaaaa
    18541 ccggcggcgg cgcggccgaa gccccggccc ctgcggcggc cccgcagccg gccagcccgg
    18601 cgcctaccgc caaagcgccg atcaagatcg acaccaaccg cccgatcagc ccggaagcgc
    18661 tgaagctggc cgaggccgag gcggtcgata ttgccgggat cgaaggctct ggccggggcg
    18721 ggcgcatcac ctatcaggat gtcgcccagg ccctgcgcgc ggcagaaggc tttgccgccc
    18781 ccgcgcccaa gggcaccgcc cagctgatcg aggacaatta caagaccttc gcctcgccgc
    18841 tggcccggcg catcgccgcg ctccatgcga ttgatctgac cggcgtgcgg ggcacagggc
    18901 cgcgcgggcg catctccaag gccgatgtgc tggcgctggt gccggcgcct gcccctgctg
    18961 ccagcggcct gtccagcggc cccttcgtgg cggtggacaa tacgcccgag gtggtgcctt
    19021 tcgacaagat ccgcaaggtg gtggcccagc gcctcaccca ggccaagcag gaactgccgc
    19081 atttctacct gcgcatctcg gcccggatgg acgagctgat cgccttgcgc aaaaccgcca
    19141 atctggtgct gggcgccaag gccagcatca acgattatat cgtcatggcc tcggccaggg
    19201 cgctggcccg ccacccggat gtgaacgtgc aggtgcacgg gcaggagatc caccgcttcc
    19261 cccatgccga tgtggcgatt gccgtggcca gccccaaggg ccttgtcacg ccgatcgtgc
    19321 ggcaggccga tcgcatgcgg atcgaccaga tcgccgctgc cacccgcgcc ctgatcgaca
    19381 aggcccaggc cggcaagctg ggctatgagg acatggccgg cggcaccttc tcggtctcga
    19441 acctgggcat gttcggcatc gagaatttcg atgcgatcat caatccgccg cagggcgcca
    19501 ttctctcggt gggcacgatc ctgcgcacgc cggtggaaac cgccagcggc atcgccttcg
    19561 aaagccggat cagcttcacc ctctcggtcg atcaccgggc gatcgacggg gcggccggcg
    19621 cccagttcct ggccacgctg aagggcttgc tggaggagcc ggaaagcctg ttcgcctgag
    19681 gaggaggcgc atccccccgt gccgatggcg cggggggatg tttttcagcc gccgaaagcc
    19741 gccgccagcg cctctggccc ggcacacacg cccttttcgg tgatatagcc tgtcaccagc
    19801 gcggccggcg tcacatcgaa ggcggggttg cgcgcgggcg ccgcggtgat cgccaccttg
    19861 cgcatcacgc ctgcttcatc cggcccgctc acaaagcgca gttcatcgcc cgaacgttcc
    19921 tcgatgggaa tgccatcgcc cgatggcgtg gccggatcat aagtgctggc cggcagcgcc
    19981 acataaaagg gcacgccatg cgcgcgcgcc gccagcgcct tcagataggt gccgatcttg
    20041 ttgcacacat cgccattggc cgccacgcga tcggtgccga caatcaccat atccaccagc
    20101 ccgcgctgca tatagagccc gccggcatta tcggcgatga agctgaccgg cacgccatga
    20161 tcggccagtt ccagcgccgt cagcgcgccc tggttgcgcg gccgggtttc atccacccac
    20221 acgtgcagcg ccagcccctc gtcctgcgcc agatagagcg gggcggtcgc cgtgccccag
    20281 gccaccgttg ccagccaacc ggggttgcaa tgggtgagga tgttgaccgg ctcgcccggc
    20341 gcctttgcag cggccagggc gcggatcagc ggcaggccat gggcgccgat ggcgcggttc
    20401 accgcctcat cctcctcgca gatggcatcg gcctcggcat aggcggcagc ggcgcgcgcg
    20461 gccggcggca aaggcgccac ccgggcgcgc acccgctcca gcgcccagcg caggttcacc
    20521 gccgtgggcc gcgtggccgc cagcaggtca accgcccggg ccagcgccgc atcatcggcg
    20581 cccgcgcgca gggccagcgc cagcccatag gcggccgtgg cgccaatcag cggggcgccg
    20641 cgcgtggcca tgtcgcggat ggcatcggcg gcctcttcgg cgctggtcag ccgcagcgtc
    20701 tcgatcgccc agggcatgcg ccgctggtcg aagatgccca cgctccagcc atcggcctcg
    20761 cgccagatcg agcgcgaacg ggtggggctg cgctgcccgc tcatgccgca gcgcctgcgg
    20821 gcaggatcgt ggcaaaatcc accgccacag caaaatcgcc ggcaggggct gccccttccc
    20881 ggtcgagcag gatcacctgc aggcccgccg cgcgcgccgc tgccagttcg gccggcacat
    20941 cggagaggaa caggatctcc gccgccgcga tcgccgtcgt ctcgccaatc gcgcgggcaa
    21001 tggcggcata ggaggcagcc tccttcttgc cgcccacgcc cgtgtcgaac cagccggcaa
    21061 acaggccggt cagatcgccc gcctcgctat ggccgaacag cagcttttgc gccgccaccg
    21121 agcccgagga atagacatag agcttcagcc ccgccgcctg ccagcgcgcc agcccggccg
    21181 ccgcatcggc atagatatgg cccttcagcg tgccatcggc atagccctcg gcccagatca
    21241 gcccctgcag ctccttcagc gggccgatct tggcatcggc atcggcccag gccagcaatt
    21301 gcgccacgca agcctgctca tccagcccgg catcgcccgc ctctgcccgc acccgcgcca
    21361 gcagcggcgc cagatcctgc gcatgggccg ccacataagc ggcaagccgc gcccgggcat
    21421 aggggaacag cacctcggcc acaaaggcga tggaactggt ggtgccctcg atatcggtga
    21481 cgatggcgcg caccgggctc atgccgccgc tcctgcgtaa agcgggatgg cgctggcgat
    21541 ggcgtcgcca gtgaagcggg ccacccagcc ttcgggcgtg gtgaacaggc ggatggccgt
    21601 gaagcggggc gcagcgccgg tgtcgaacca gtggcgcgtg ccggccggca gcacgatcag
    21661 gtcgccggcc gtgcattcca gcgccagcac ctgcccgcca tggcggatat agaacagccc
    21721 cgctccttcc acaaagaagc gcacctcgtc atcatcatgc acatgttcgg cgaggaattt
    21781 ggcgcgcatg tcagccgccg ccggatgatc gggcgtcagc cgcacgacat cgacgctacg
    21841 atagccgcct cgcgcctgca aggccgccac ctgcggcgca taggccgcca ggatcgccgc
    21901 atccgccggc gcatcgccca gcgcggcacc cgcctgccag cgctccagcc cgatgccggc
    21961 ctcggccagc acggcgctga tatcgccgaa atcggcgctg ctgtcgatct ctgcatgggg
    22021 cgcctgatcg gcatagatgg ccagatgggt catcgccccg ctcccttcag cttcagctct
    22081 tcccaggcgc aggccagcag gaattcgcag ccctccacca ggttttccgc cgcatccatc
    22141 gttgcccccc aggcataaag gccatggccg cgaatgtaga aggccggcag gatcggcgtg
    22201 cgcgcggcga tgatcgggcg cagctgatcg gccagcgctg ccatatcctg cgtgttatcc
    22261 accagcggca tggcgatggt gcaatcatgc gtggcgcagc cgggaaagat cttctgcact
    22321 tcatagccgg tgaacaggat cgccgcgccc gccgtcgccc ggctgagcac cgtgccggcg
    22381 atcgaatggg tgtgcagcgc ggcccccgcc gcgccgtcca ccgcatagat caggcagtgc
    22441 agcagcgttt cggccgaggg cttctttgcc tccagcgcct cgccggtggc actggcgcgc
    22501 atgatttcgg ccgccgtcaa ccgccccttg tgcgcgcccg acacggtgac ggccagcgtg
    22561 ccatcctcca gccggatcga ataattgccg gccgtggcag gcgccagccc gcgggcatcc
    22621 agccggcggc cggccgcgca gatcaatgct gcggcctcgt catacatcat gccctgttgc
    22681 tccgaaattg gctgaggcgg gccaccgcct cgtcaagcac atctgccgat ttggcaaaac
    22741 acaggcgcaa ataacccgtt tgcgccgctt cctcatagaa cgccgaaacc gggatcgagc
    22801 cgaccccgcc gccgcgcacc agctcctcgg ccagggcggc atcctctgcc ggcaagccgc
    22861 tggccagcag atccaccagc acgaaccagc tgccgctgtt gggcagcacc gcaaagccgg
    22921 ccccggccag cccctccacc agccgcgccc gggccgccgc atagcgcgcg cgttcgcgcg
    22981 catgccaggg cgccggcagc gccagcccct ctgccaccgc ccattgcagc ggcgtgggcg
    23041 tggtgaaggt gaggaattga tggatcgccg ccacttcctc ggccagcgcc ggatcggcca
    23101 ccacccagcc cagcttccag ccggtgagcg agaaaatctt gccggcagag cccaccttca
    23161 ccgtgcgctg ccgcaggctg tccaccgcca gcgccgacag cggcgccgcc ccatcgagca
    23221 gcgttgcctc ccacacctcg tcggacagga cgacgaggcc gtgctctgcg caaagggcgc
    23281 caatgccttc cagcgtcgcc cggtcgagca gcgtgccggt ggggttgttg ggcgtgttga
    23341 ggatcagcag ccgggttttc ggcgtgatcg ccgcggccag atccgccagc tccagccgcc
    23401 attccggcgg ccgcaggctg accagccgca ccaccccgcc cgcccggcgc accagcggca
    23461 cataggcatc ataaagcggc tgcacgcaga tcacctcgtc gcccggccgc accagcgcca
    23521 gaatgctggc cgccagcgcc tcggtcgcgc cagaggtgac gatcacttcg gccggatcca
    23581 ccgccagccc ctgtgcggcg ccataataag cggcgatcgc cgcgcgcaat tgcggcagcc
    23641 cgcgcatcgg tggatattgg ttggaatgtt cggccagcgc ccgctgcgcc gccgcgatca
    23701 gctcggccgg ctcgcccatt tcggggaagc cctggcccag gttgatcgcc cccagctgcc
    23761 ggcacaggcc cgacatatgc tcaaaaatcg tcgtcttgcg ggcagatccg gtcattccag
    23821 ctctcttgca gcgccaatgt gatggttttg cgacagatag gctgaggccg cccgcccgcg
    23881 caagccgctc acagcaaagc ggggagcagc ttgcgccact ccccgcctgc attgtgtcag
    23941 ctgataaagc cgtgctgcgc ccaaaggcgc cgcggcctta ttcggcgtct tcagccacca
    24001 gcaccgggcc cgaatcctgg cccttggcag ccacgtcgcg gtccaccagc tcgatcacgg
    24061 cgatcggggc ggcgtccgaa gcgcggatgc cagccttgat gatgcgggtg tagccaccgc
    24121 tgcgcgcggc atagcgggcc gccagcacgt cgaacagctt caccagctgc gcatcgtcct
    24181 gcaggcgggc atgggccaga cggcggttcg acaggccgcc acgcttggcc agcgtgatca
    24241 gcttctcgat gtaggggcgc agttccttgg ccttgggcac cgtggtggtg atctgctcat
    24301 gcttgatgag cgaggccgac aggttgcgca gcagcgcggt gcggtgctgg ctggtgcgct
    24361 gcagcttacg ctggccgagt ttatgacgca taattcattc cttccgttcg ttaagggccc
    24421 gtgtaaggta gcccagaccc ggcggcgctt gacgggggcc gcccctgccc ttgcggcagc
    24481 gagagccacg cccccgccgc catcaggccc ttcagccgag cagttcctgc tccagcttct
    24541 tggccatttc ctcgatgttc tccggcggcc agcccgggat atccatgccc aggcgcagcc
    24601 ccatggagga cagcacttcc ttgatctcgt tgagcgactt gcggccgaag ttcggcgtgc
    24661 gcagcatctc cgcctcggtc ttctgcacga gatcgccgat gtagatgatg ttgtcgttct
    24721 tgaggcagtt ggccgaacgc accgacagtt ccagctcgtc caccttcttg aggaggtagc
    24781 ggttgagctg gttggtgtcg ccctcttccg gcgcggccgt ggcaaggccg gcaatcgcgg
    24841 gcacgccgat cggcgccaca tcctcgaagt gcacgaacag cgccagctgg tcctgcagga
    24901 tgcgcgcggc ataggccacg gcgtcttccg gggtcacggt gccatcggtc tcgaccgtca
    24961 ggttcagctt gtcgtagtca agctcctggc cgatacgggc gttgtccacc ttgtacgaca
    25021 cctggcgcac cggcgaatag agcgaatcga ccgggatcag gccaatcggc gcatcggccg
    25081 ggcggttcga cacggccggc acatagccct tgccggtgtc cgcggtcagc tccatgttca
    25141 gcgtcgcgcc ttcatcgaga tggcagatca ccagatcctt gttcatcacc tcgatgtcgc
    25201 cggtcacggc gatgtcgccg gccttcacca cgcccgggcc agtggccgaa agctggaggc
    25261 gcttggggcc ttcgccctgc atcttcagcg cgatctgctt cacgttgagg acgatgtcgg
    25321 tcacatcctc gcgcacgccg gccagcgagc tgaattcgtg cagcacgttc tcgatcttga
    25381 tcgaggtgat cgccgcgccc tgcagcgagg agagcagcac gcggcgcagc gcattaccca
    25441 aagtcaggcc gaaacccctc tccagaggct ctgcaacaaa ggtggctcgg cgcttgggat
    25501 ccgtgcctgg cttgatttcc aggctattgg gcttcttcag ttcctgccag ttcttgatgt
    25561 tgacagtcat ggacttcccc tggggttatt ctggggggaa aggccgcaag cgcaccgaaa
    25621 atgcaccgcg gcctatccga aaaaacgttg cgggcggccg gccagccacc cgcgaaatgc
    25681 catcagacgc ggcgacgctt cgaagggcgc acaccgttgt gcgggatcgg cgtcacgtcg
    25741 cggatggagg tgatggtgaa gcccaccgcc tgcagggcgc gcagggcgct ctcgcggccc
    25801 gaaccggggc ccttcacttc cacttccagc gtgcgcacgc cgtgttcggc ggccttcttg
    25861 ccggcatcgt cagcggcgac ctgggcagca taaggggtcg acttgcgcga gcccttgaag
    25921 cccatcatgc cggccgacga ccagctgatg gcgttgccct gggcatccgt gatggtg
//
LOCUS       DASRYE010000348         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_70681,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000348 DASRYE010000000
VERSION     DASRYE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70681"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFF98_18205"
     CDS             complement(<1..951)
                     /locus_tag="VFF98_18205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZU65628.1"
                     /translation="MNRRINTDSDMSLANIPSLSANMARCGGLALALALAACKAGPDY
                     HGPAAAVPAPAAFARASGIAGETGAAPAPRWWHGLGDPVLDALEAQALAGNPSLAAAA
                     ARLGQARASLKQDKARLLPTVGAMGAVMHADLPGTGIGGVDTLNFYNAALDASWELDL
                     FGKQRRTVEAGRASADAAADRVADAQVSLAAEVAQDYVLLRQRQQRLAAQRHVVERLE
                     AEMGLVRQRVAQGTAADADVARLARQLEEARQALATLSADRAAYLDALAVLAGVAPGA
                     LDALLAPVGVDEGHLAVPLPPAAVAVGDPARLIAHRPDVRA"
     gene            complement(964..>2129)
                     /locus_tag="VFF98_18210"
     CDS             complement(964..>2129)
                     /locus_tag="VFF98_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZU65629.1"
                     /translation="TIIATRLPHHQQPVGNALFGVTAILGPVLGPLIGGWLTENWSWH
                     YAFFLNLPVGCVLVGLLLIGLPHKRPHWEEFWNADWIGILGLAIGLGSLTVVLEEGQR
                     EQWFQSAEIVWLTVSSGLGLCLLLLGQFVARRPVIKLKLLLDRQFGAVAVMGVVVGMM
                     LYGTAFVIPQFLAAVDDYNALQSGQVVLISGLPSLAMMPLTPLLIRNIDIRISVSIGF
                     AMMFTSCLVDTDMSALTSGHDFVLSQLLRGMGSVFAFLFLNQAAIASVPRHEAGDAAG
                     LFNSARNLGGSLALAGIAVLQDQRQWLHSRRLEDTLSANSDRVQDYLAALAQNAGGNQ
                     AAAIRQLGGTIAREALVMTYNDIFWLLAMGILFVSPLVLLLRPLPKGPIAAAH"
BASE COUNT          371 a          792 c          690 g          276 t
ORIGIN      
        1 cgcgcgcaca tcggggcgat gggcaatcag ccgcgccgga tcgcccacgg ccaccgcggc
       61 cggcggcagc ggcacggcca gatggccctc atccaccccc accggcgcca gcagcgcatc
      121 cagcgcgccc ggcgccacgc ccgccagcac cgccagcgca tcgagatagg cggccctgtc
      181 ggccgagagc gtggccagcg cctggcgtgc ctcttccagc tggcgggcca ggcgcgccac
      241 atcggcatcg gccgcggtgc cctgcgccac gcgctggcgc accaggccca tctcggcctc
      301 cagccgttcc accacatggc gctgggcggc cagccgctgc tggcgctggc gcagcagcac
      361 ataatcctgc gccacttcgg cagccagcga cacttgcgca tcggccaccc ggtcagccgc
      421 agcatcagcg ctggcgcggc cggcttccac cgtgcggcgc tgcttgccga acaggtccag
      481 ctcccagctg gcatcaaggg cggcgttgta gaaattgagc gtatcgacgc cgccaatgcc
      541 ggttccgggc agatcggcat gcatcaccgc gcccatcgcg cccaccgtgg ggagcaggcg
      601 ggccttgtcc tgcttcaggc tggcgcgggc ctggcccagc cgggccgcag ccgccgccag
      661 cgagggattg ccggccagcg cctgcgcctc cagcgcatcg agcaccggat cgcccagccc
      721 atgccaccag cgcggcgcgg gcgcggcgcc tgtctcgccg gcgatgccgc tggcgcgggc
      781 aaaggcggcc ggcgccggca ccgcggcggc tggcccgtga taatcgggcc ccgccttgca
      841 ggccgccagc gccagcgcca gcgccagccc gccacaccgc gccatgttgg ccgaaagaga
      901 agggatattg gcaaggctca tatcgctatc cgtattgatc cgtctgttca tggccgcgcc
      961 cgttcaatgc gccgccgcga tcggcccctt gggcagcggg cgcagcagca gcaccagcgg
     1021 gctgacgaag aggatgccca tcgccagcag ccagaagatg tcgttatagg tcatcaccag
     1081 cgcctcgcgg gcaatggtgc cgcccagctg gcggatggcc gccgcctggt tgccgccggc
     1141 attctgcgcc agcgccgcca gataatcctg cacccggtcg gaattggccg acagcgtgtc
     1201 ttccagccgg cggctgtgca gccactggcg ctgatcctgc agcacggcaa tgcccgccag
     1261 cgccagcgat ccgcccaggt tgcgcgccga attgaacagg cccgccgcat cgcccgcctc
     1321 gtgccgcggc accgaggcga tcgccgcctg gttgaggaac aggaaggcaa acaccgatcc
     1381 catcccccgc agcaattgcg agagcacgaa atcatggccc gaggtcagcg cgctcatgtc
     1441 agtgtcgacc aggcagctgg tgaacatcat ggcaaagccg atggagaccg aaatgcggat
     1501 atcgatgttg cggataagca gcggcgtcag tggcatcatg gccaggctgg gcaggcccga
     1561 gatcagcacc acctgccccg attgcagcgc gttgtaatca tccaccgccg ccaggaactg
     1621 ggggatgacg aaagccgtgc catacagcat catgcccacc accacgccca tcaccgccac
     1681 cgcgccaaac tgccggtcga gcagcagctt cagcttgatg accggccggc gcgcaacgaa
     1741 ctggcccagc agcagcaggc acaggcccag ccccgagctg actgtcagcc agacgatctc
     1801 ggccgactgg aaccattgct cgcgctggcc ctcttccagc accaccgtca ggctgccaag
     1861 gccgatggcc aggcccagga tgccgatcca gtcggcgttc cagaactctt cccaatgcgg
     1921 gcgcttgtgc ggcaggccga tcagcagcag gcccaccagc acgcagccca ccggcaggtt
     1981 gaggaagaag gcatagtgcc acgaccagtt ttccgtcagc cagccgccga tcagcggccc
     2041 cagcaccggc cccagaatgg ccgtgacgcc gaacagcgca ttgcccaccg gctgctggtg
     2101 atggggcagg cgggtggcga tgatggtca
//
LOCUS       DASRYE010000349         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U13048 k141_70682,
            whole genome shotgun sequence.
ACCESSION   DASRYE010000349 DASRYE010000000
VERSION     DASRYE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970276
            Sequence Read Archive: SRR7471704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 01:03:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,644
            CDSs (total)                      :: 3,601
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70682"
                     /isolate="SMAG_U13048"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="Puerto Rico"
                     /lat_lon="18 N 65 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1602
                     /gene="pgi"
                     /locus_tag="VFF98_18215"
     CDS             73..1602
                     /gene="pgi"
                     /locus_tag="VFF98_18215"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZU65630.1"
                     /translation="MARSEATKAAWDHLASLPRPTLKALFAEGGDRLGLLSTRLELGE
                     ALGGGIRFDWAKTHLSPELVTGFEALAEAVDFDGARAALFSGEKVNVTEGRAAEHTAQ
                     RGVGRESSVEEAGALHARMRSLVEAIHGGLLGEVKHLIHIGIGGSALGPALAVDALTR
                     DGAVVDVHVVSNIDGCALEAAFRACDPATTLIAVASKTFTTIETMTNAHSALDWLRGN
                     GVEDPYGRVVALTAAPDKAVEWGVDETRILPFAESVGGRYSLWSSIGFPVALALGWDG
                     FADFLEGAAVVDRHFLDSDGAANLPLRAAFADQMYTRLFGCQSRACFAYDERLALLPF
                     YLQQLEMESNGKRVKADGSPVDGATAPITWGGVGTDAQHAVFQLLHQGTVVVPVDFIA
                     AIAPGDELDPAHHRILLLNCFAQGAALMAGRDAADGARAYPGDRPSATILVDELTPAV
                     LGALIAFHEHRTFANAVLMEINPFDQFGVELGKEIAKSIEKGVAAFDPSTTALLAAAG
                     L"
     gene            complement(1666..2079)
                     /locus_tag="VFF98_18220"
     CDS             complement(1666..2079)
                     /locus_tag="VFF98_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZU65631.1"
                     /translation="MRLSSCLPLAALACIALAPAARAATDQPVCAAADTVIYRVSELK
                     PGGSLAGFAKAVADHIKWYADHGYTADVFSWGQVVDYDQASHTGKLSPTRVFTLHQHA
                     AGVPHAQQDAGWAAFVSEYDANSNMVSMQLLCTAH"
BASE COUNT          358 a          762 c          860 g          421 t
ORIGIN      
        1 gcgcgggcag ggcatagccg gcaggcaggg cgccgatgat ggcgcctgct gacgcattca
       61 aggagtttgc acgtggcgcg tagcgaagca accaaggccg cctgggacca tctggccagc
      121 ctgccccggc ccacgctgaa ggcgctgttt gccgaagggg gggatcggct gggcctgctc
      181 tccacccggc tggagctggg cgaggcgctg ggcggcggca tccgcttcga ttgggccaag
      241 acgcatctct cgcccgaact ggtcaccggt ttcgaggcgc tggccgaagc ggtggatttc
      301 gatggcgcgc gcgccgcgct gttcagcggc gagaaggtga atgtgaccga gggccgcgcg
      361 gccgagcaca ccgcccagcg cggcgtgggc cgcgagagca gcgtggagga agccggcgcg
      421 ctccatgccc gcatgcgcag cctggtggaa gcgatccatg gcggcctgtt gggcgaggtg
      481 aagcatctca tccacatcgg cattggcggc agcgcgctgg gcccggcgct ggcggtggac
      541 gcgctgaccc gcgacggggc ggtggtcgat gtccatgtcg tctccaatat cgatggctgc
      601 gcactggaag cggcgtttcg cgcctgcgat ccggccacca cgctgatcgc cgtcgcttcc
      661 aagaccttca ccaccatcga gacgatgacc aacgcccatt ccgcgctcga ctggctgcgc
      721 ggcaatggcg tggaagaccc ttatggccgc gtggtggcgt tgacggccgc gcccgacaag
      781 gcggtggaat ggggcgtgga tgaaacccgc atcctgccct ttgccgaaag cgtgggcggg
      841 cgctattcgc tgtggagctc gatcggcttt ccggtggcgc tggcgctggg ctgggatggc
      901 tttgccgatt tcctcgaagg ggcagcggtg gtcgatcgcc acttcctcga cagcgatggc
      961 gcggccaacc tgcccttgcg cgccgccttt gccgaccaga tgtatacccg cctgttcggc
     1021 tgccagagcc gcgcctgctt tgcctatgac gagcgcctgg cgctgctgcc cttctatctg
     1081 cagcagctgg agatggaatc gaacggcaag cgcgtgaagg ccgatggcag cccggtggat
     1141 ggcgccaccg cgccgatcac ctggggcggc gttggcaccg atgcccagca tgcggtgttc
     1201 cagctgctgc atcagggcac ggtggtggtg ccggtggatt tcatcgccgc catcgcgccg
     1261 ggggacgagc tggacccggc ccatcaccgc atcctgctgc tgaactgctt tgcccaaggg
     1321 gccgcgctga tggccgggcg cgatgccgcc gatggcgccc gcgcctatcc cggagaccgc
     1381 ccttcggcga ccattctggt ggacgagctg acgccggccg tgctgggcgc gctgatcgcc
     1441 ttccatgaac accgcacctt tgccaatgcc gtgctgatgg agatcaaccc gttcgatcag
     1501 ttcggcgtgg aactgggcaa ggaaatcgcc aagtcgatcg agaagggggt ggcggccttc
     1561 gatccttcaa ccacggcgct gctggcagcg gccgggctct aggccttgcg gggcagcgcc
     1621 ggcccagtgg gcggcgctgc cccattcggg cttagtgggc caggcttagt gggcggtgca
     1681 cagcagctgc atgctcacca tgttgctgtt cgcgtcatac tcgctgacga aagcggccca
     1741 gccggcatcc tgctgggcgt gcggcacgcc cgccgcatgc tggtgcaggg tgaacaccct
     1801 ggtgggcgag agtttgccgg tgtggctggc ctgatcgtaa tccaccacct ggccccagct
     1861 gaacacatcg gcggtatagc catggtcggc ataccatttg atgtgatcgg ccaccgcctt
     1921 ggcaaagccg gccagcgatc cgcccggctt gagctcgctg acgcggtaga tgacggtatc
     1981 ggcagcggcg cagactggct ggtctgtcgc ggcgcgggcg gcgggcgcca gggcgatgca
     2041 ggccagggcg gcaagcggca ggcaggatga aaggcgcatg ttgcaattct ccccattttg
     2101 tagctggccg cgattatgcc cgccgtggat ggcggtttgt agacacagct gaggtcaaat
     2161 ccgcccttcc gggcgcaact gcatcggttt cggcagtgat gcagcggggt tgatcttcgc
     2221 ccgggcaagc gccacttctc gccggcagct gtggcgggcg ccccctttgg cccgcggcga
     2281 tggaaggctt ttatggcatt tgactatgat ctgttcgtga tcggcgcggg ctctggcggt
     2341 gtgcgcgccg gcaggctcgc cgccgagctc ggcgccaagg tggccatcgc cgaggaatat
     2401 c
//
